LOCUS       DATAOZ010000001         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2367722, whole genome shotgun sequence.
ACCESSION   DATAOZ010000001 DATAOZ010000000
VERSION     DATAOZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367722"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VHR64_00005"
     CDS             <1..1158
                     /locus_tag="VHR64_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX3301235.1"
                     /translation="VAEQTLDYVLREMTSEKGGFYAAQDADSEGEEGKFYVWTPDEIR
                     SVLSPEDAALAERYFGVVEGGNFEGKSILTLAGDPAEHSDEVRRIREQLYEARAKRVW
                     PHRDEKIITSWNGMTIRALAEGGLVLNRPDFIDAARKCATFLRRDVDVEGVLHRTYGG
                     GSARIPAFLEDYAQLADGLMALHEATLDLEWLQWAQELIGRMVALFADGAGDGFYDTS
                     SQHDQLAIRPRELQDGATPCGSSVASDVLIRMGQITSDESLVERAQTVLRSMRDFMED
                     QPLGFGRYLSAGCRLLDTLREIAIAAPDGDDGIREFQEVVYRRFEPNAVIGLVSDEAQ
                     AVMPWLADRPIRDSRATAYLCEQFVCLPPVTVPADLTMQLEMGTGMSWQAF"
     gene            complement(1155..1937)
                     /locus_tag="VHR64_00010"
     CDS             complement(1155..1937)
                     /locus_tag="VHR64_00010"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3301236.1"
                     /translation="MRIVRFRDGSSVYWGIAREGYVQHMPEAPFWFSDDGEIAGKIDE
                     LALLTPVDPGKIVCVGLNYEAHVTERDPNRKVPTEPVLFMKPPSALIGNGEEIRIANP
                     DHETHHEAELVVVIGKRAHCVDVESSRDYVLGYTCGNDVSDRDLQAQDGQFVRAKGFD
                     TYAPIGPWIETKLDPTKLKISCDVNGEIRQNSSTSKMIWGVDALISFISHVMTLEPGD
                     IIMTGTPHGVSRLNTGDVCTIAIQGIGSLENPVRNRSEVNGG"
     gene            2031..>2720
                     /locus_tag="VHR64_00015"
     CDS             2031..>2720
                     /locus_tag="VHR64_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301237.1"
                     /translation="MPDLYLQLSDDPFDGPSEIPALTEDEVLEVLREGEFVGSKLIPW
                     GSNYSFAVAIMDAEGREQLAIYKPRDGENPLYDFPQGTLYLREVAAYRLSKLLGWNIV
                     PPTVVSEGPMGIGSLQLYKSPVERNDDCDPREYWSRREIDVERLVLFDHVANNADRKL
                     SHCLLDATGAVWGIDHGLCFNADMKLRTVLWQFVGGPVNATLIADLATLRCREPELRA
                     ELDELLEPREIN"
BASE COUNT          485 a          824 c          892 g          519 t
ORIGIN      
        1 gtcgccgagc agaccctcga ttacgtcctc cgcgagatga cgtccgaaaa gggcggtttc
       61 tatgccgcgc aagatgccga cagcgagggc gaagagggca agttttacgt ctggacgccg
      121 gatgagattc ggtctgtcct gtcgccggaa gatgcggcgc ttgccgagcg gtacttcggc
      181 gtcgtcgagg gcggcaactt cgaggggaaa tcgatcctga cacttgccgg cgatccggcc
      241 gagcactccg acgaggtcag gcggatccgc gagcagctct acgaagcacg ggcgaagcgg
      301 gtctggccgc accgggacga gaagatcatc acgagctgga acggcatgac gatccgcgcc
      361 ctggccgagg gtgggttggt cctgaatcgg ccggacttca tcgatgcggc ccgaaagtgc
      421 gcaacctttc tccgccgtga tgtcgacgtg gagggcgtcc tccaccgcac atatggcggc
      481 gggtcagcgc ggattcccgc tttcctcgag gactatgccc agctcgctga tggattgatg
      541 gcgctccatg aagcgacgct cgacctggaa tggctgcagt gggcacagga gctcatcggc
      601 cgtatggtcg cgctctttgc cgacggggcc ggagacggct tctacgacac gagctcacag
      661 cacgaccaat tggcgatccg gccgcgggag ctccaggatg gggcaacgcc gtgcggatct
      721 tcggttgcga gcgatgtcct gatccggatg ggccagatca cgagtgacga gtcgttggtc
      781 gagcgggccc aaactgtgct gcgctcgatg cgggatttca tggaggatca gccgctcgga
      841 tttggcaggt acctgagcgc cggctgccgg ttgctggaca cgttgcgcga gatcgcgatt
      901 gccgcgccgg acggcgacga cgggatccgg gagttccagg aggtcgttta ccgcaggttc
      961 gagccaaatg ccgtgattgg cctggtgtcg gacgaggcgc aggcagtcat gccctggctg
     1021 gccgaccggc cgattcgtga cagccgggcc accgcctatc tctgtgagca gttcgtctgc
     1081 ctgccgcccg tgaccgtgcc ggccgacctg acgatgcagc tcgagatggg caccggcatg
     1141 tcctggcagg ccttctaacc gccgttcact tccgagcggt tgcggaccgg gttctcgagc
     1201 gagccgatgc cctgaatcgc gatcgtacag acatcgccgg tattgagccg gctgacgccg
     1261 tgtggcgtgc cggtcatgat gatgtcgccc ggctcgaggg tcatcacgtg cgagatgaac
     1321 gaaatcagcg cgtcgactcc ccagatcatc ttgctggtcg atgagttctg ccggatctcg
     1381 ccgttgacat cgcagctgat cttgagcttg gtcggatcga gcttcgtctc gatccacggc
     1441 ccgatcggcg cgtacgtgtc gaagcccttg gcgcgcacaa actggccgtc ttgcgcttgc
     1501 agatcgcggt cggaaacatc gttgccgcag gtgtagccga gcacgtagtc gcggctggac
     1561 tctacatcca cgcaatgggc acgcttgccg atcacgacga ccagttcggc ttcgtggtgc
     1621 gtttcatggt cagggttcgc gattcggatc tcctccccat tgccaatcag ggcagagggc
     1681 ggtttcatga agaggaccgg ctccgtgggg actttgcggt tcgggtcgcg ctcggtaacg
     1741 tgcgcttcgt aattgaggcc gacgcagaca atcttgccgg ggtcgaccgg cgtaagcaaa
     1801 gcgagttcgt cgatcttgcc ggcaatctcg ccgtcgtcgc tgaaccagaa gggtgcttcc
     1861 ggcatatgct gaacgtagcc ctcccgggca attccccagt agaccgacga tccgtcccga
     1921 aacctgacga ttctcattcc gtcctcccgg ccacatcgcg acccgaacct actcgcgctg
     1981 gttgaacctt gccaataacg cgaaactagt accacgatca ggagcgacct gtgccggact
     2041 tgtacctcca attgagcgat gaccccttcg atggcccgtc cgagatcccg gcattgaccg
     2101 aggacgaggt cctcgaagtg cttcgcgagg gcgagttcgt cggctcgaag ttgatcccgt
     2161 ggggatcgaa ctattccttc gcggtcgcga tcatggacgc cgagggtcgc gagcagttgg
     2221 cgatctacaa accgcgcgac ggcgagaacc cgctatacga cttcccgcaa ggcacgctct
     2281 acctgcgcga ggtcgcggcc taccggttgt cgaagttgct gggatggaac atcgtgccgc
     2341 cgacggtcgt gagtgaaggg ccgatgggga tcggtagcct gcagctctac aagtcgccgg
     2401 tcgagcgaaa cgacgactgt gatccgcgcg aatactggtc acgccgggag attgacgtcg
     2461 agcgactggt ccttttcgac catgtcgcca acaacgccga ccggaagctc tcgcattgcc
     2521 tgctcgatgc cactggagcg gtctggggaa tcgatcacgg actctgtttc aacgcagaca
     2581 tgaagctgcg cacggttctt tggcagttcg tcggcgggcc ggtcaatgcg acgctcatcg
     2641 ccgatctggc gacgctccgg tgccgggagc ccgagctccg cgccgagctc gacgagctgc
     2701 tcgagccgcg ggagatcaat
//
LOCUS       DATAOZ010000002         4098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1053195, whole genome shotgun sequence.
ACCESSION   DATAOZ010000002 DATAOZ010000000
VERSION     DATAOZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053195"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /locus_tag="VHR64_00020"
     CDS             complement(<1..2212)
                     /locus_tag="VHR64_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="HEX3301238.1"
                     /translation="MHAPDSHCLTIPSLSLVALIGVSGSGKSSFARRHFLPTEVVSSD
                     FCRGLVSDDENSQEATADAFDVLNYIAGKRLKRGLLTVIDATSVQREARASLVRLARE
                     YHAIPVAIVLNLPERICRERNATRPDRDFGPHVIRRQSEALRRSLRNLQKEGFRYVYI
                     LNSPEEVEAAEIIRQPLWVDRRTDHGPFDIIGDLHGCLDETLELLAALGYRVEIGTAD
                     DGGRRFDVSAPHGRRAIFLGDLTDRGPDAPGTLRLVMDMVAAGVAFCVPGNHDDKLLR
                     KLKGRNVRINHGLDLTLAQLEQEPPEFSRRVATFLDSLVSHYVLDDGRLVVAHAGLIG
                     AYQGRASRTVRDFALYGDVTGETDDDGFPVRGNWAADYRGSAMVVYGHTAVAEAEWLN
                     NTIDIDTGCVFGGRLTALRYPERELVSVPAHATYADAGPGFRTEPADQQSLSAQQAHD
                     EVLDVADLLGRRMIETGLMRLVTIPEERGLAALETMSRFAVNPQWLIYLPPTMSPVET
                     SSEPDLLEHPAEAFAYFRARNVPLVIAEEKHMGSRAVLVICRDADAARRRFGITDGSD
                     GIIYTRTGRRFFDAKTEGEVLTRLRAAMTGSGLWDELNTDWVCLDAEIMPWSAKAQDL
                     LRHQYAATGSAAAADLSATLDALTRAQNQGVDVSALLQRTQKRMTAVTAYVDSYRRYV
                     WSVESVADLRIAPFHLLASAGAAHTSQTHSWHLEQARKLAAADPGVILATASLDV"
     gene            complement(2240..3679)
                     /locus_tag="VHR64_00025"
     CDS             complement(2240..3679)
                     /locus_tag="VHR64_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="HEX3301239.1"
                     /translation="MLLTISTTHQPATDLGYLLHKYPGRCQSFSLSFGEAHVYYPEAT
                     DSRCTAALQVEVDPIRLVRRGPGSARFALAQYVNDRPYVASSLLSVAIGDVYRSSLIG
                     QSRDRADLALTPIPLEIGLSAIRCRGGEAVLRRLFEPLGYDRIEAVQLPLDERFPEWG
                     SGPAYCVTLGVTARLQDVLRQLTVLIPVLDDDKHYWFGEDELDKLLRRGEGWLDQHPE
                     RKLIADRYLRYRRALVRHAIERMAISDGTDPDGQDSSAPNLEETVERPISLHEERLKA
                     VAAVLRDRGVQRVVDLGCGEGKLILQLVEDRQFTEILGVDASSIALAHAARRIHLDRW
                     PAEGRVRLIHGALTYRDKRLAGFDAAAIVEVIEHLDPFRLAAFERVVFESARPGLVVV
                     TTPNQEYNQLFDSLASGSFRHPDHRFEWTRPEFASWAEGVADRFGYAIELAGIGPVDP
                     DHGQPSQMAIFSRPQKSKPETQTAEPISA"
     gene            complement(3807..>4098)
                     /locus_tag="VHR64_00030"
     CDS             complement(3807..>4098)
                     /locus_tag="VHR64_00030"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX3301240.1"
                     /translation="LYLVNGPDAGRPTTLDQMTADGGQSIDVSWHSIVEGLGNLEATS
                     IILLGLLILILTPLTRVAMTFVLFILQRDRIFVAITGIVLVILILGFIGIGA"
BASE COUNT          724 a         1345 c         1370 g          659 t
ORIGIN      
        1 ccacatccag gctcgccgtc gcgaggatca ctcccggatc ggcggcggcg agtttccgcg
       61 cctgctccag gtgccagctg tgggtctggc tggtgtgtgc cgcccccgcg ctggcgagca
      121 gatggaacgg cgcgatgcgg agatcggcga cagactcgac cgaccagaca tagcggcggt
      181 acgaatccac gtacgcggtc accgccgtca ttcgcttctg cgtgcgctgc aagagggccg
      241 acacatcgac gccctgattc tgcgcacggg tcagtgcatc taatgtcgcg gacaggtctg
      301 ccgccgcggc tgagccggtg gccgcatact ggtggcgaag caggtcctgc gccttggccg
      361 accacggcat gatctccgcg tcgaggcaga cccaatcggt attcagctcg tcccagagac
      421 cggatccggt catggccgcc cgtagccggg tcagcacctc gccctcggtc ttggcgtcga
      481 agaagcggcg gccggtgcgg gtgtagatga tgccatcgct cccgtcggtg atcccgaatc
      541 ggcgccgggc agcatcggca tcccggcaga tgaccagaac ggcgcgcgag cccatgtgct
      601 tctcctcggc aatcaccagc gggacattcc gcgcccggaa gtacgcgaac gcctcggcgg
      661 gatgctcgag cagatcgggc tcgctcgacg tctccaccgg agacatcgtc ggcggcaggt
      721 agatcagcca ctgcgggttg accgcgaacc ggctcatcgt ctcgagtgcc gccagtccgc
      781 gctcctcggg gatggtcacg agccgcatca ggccagtctc gatcatgcgt cggccaagca
      841 ggtcggccac gtcgagtacc tcgtcatggg cctgctgcgc agagagcgac tgctggtcgg
      901 cgggttccgt ccgaaatccc ggacctgcgt ccgcgtacgt cgcgtgtgcc gggaccgaga
      961 cgagctcgcg ttccgggtag cgcagggcag tcagacgccc gccgaagaca catccggtgt
     1021 cgatgtcgat cgtgttgttg agccactcgg cctcggcgac ggcggtatga ccgtaaacga
     1081 ccattgccga gccgcgatag tcggctgccc agttaccccg cacggggaat ccatcgtcgt
     1141 cggtctcgcc ggtgacatcg ccgtagaggg cgaagtcgcg gacggtgcgc gaggctcggc
     1201 cctggtacgc cccgatcaac cccgcatggg cgacgacgag ccggccgtcg tccagcacgt
     1261 aatggctgac caggctatcg aggaacgtcg ccacgcggcg gctgaactca ggcggctcct
     1321 gctcgagctg cgcgagggtc aggtcgagac catggttgat gcggacgttc cgtcccttca
     1381 acttgcgcag gagcttgtcg tcgtggttgc ccggcacgca gaaggcgacg ccagccgcga
     1441 ccatatccat gaccagccgc aacgtaccag gcgcgtccgg accgcgatcg gtcaggtcgc
     1501 ccaggaagat cgcgcggcgg ccgtgcggcg cagagacgtc gaatcggcga ccgccatcgt
     1561 cggcggtgcc gatctcaacc cgatacccga gcgccgcgag cagctccagc gtctcatcca
     1621 ggcagccgtg cagatccccg atgatgtcga acgggccgtg gtctgtccgc cggtcgaccc
     1681 agagcggctg gcggatgatc tccgcggcct cgacctcctc cggcgaattc aggatgtaga
     1741 cgtaccggaa cccctccttc tggaggttgc ggagcgaccg ccggagcgct tccgactggc
     1801 gacggatgac atgcgggccg aagtcgcgat cgggccgcgt cgcattgcgc tcccggcaga
     1861 tccgctcggg caggttgagc acgatcgcga ccgggatcgc atggtattcg cgggccagcc
     1921 ggaccagcga tgcgcgcgcc tcccgctgga cactggtggc atcgatcacg gtcaacaacc
     1981 cgcgcttcag ccgcttgccg gcaatgtagt tgagcacgtc gaaggcgtcg gccgtcgcct
     2041 cctggctgtt ctcgtcatcg gagacaagcc cgcggcaaaa gtcggacgac accacctcgg
     2101 tcgggaggaa atggcgcctg gcgaacgacg acttgcccga gcccgagaca ccgatcaggg
     2161 cgaccagcga caggcttgga atcgtgaggc aatgtgagtc aggagcgtgc atctcgaact
     2221 ggcttccaat caggcgacgt tacgcggaga tgggctcggc agtttgtgtc tccggcttcg
     2281 atttctgcgg gcggctgaag atcgccatct ggctcggctg cccgtggtcg gggtcaaccg
     2341 gtccgatacc ggccagctcg atggcatagc cgaatcgatc ggcgacccct tcagcccagc
     2401 ttgcgaactc aggccgcgtc cactcgaagc ggtgatccgg gtgacgaaac gagccgctgg
     2461 caagcgagtc gaagagttgg ttgtactcct ggttgggcgt ggtcacgacg accaggcccg
     2521 gccgcgccga ttcgaagacg acccgctcga aggcggccag ccggaacgga tcgagatgct
     2581 cgatgacctc gacgatcgcc gccgcatcaa agccggcgag ccgcttgtca cggtacgtca
     2641 gcgcgccgtg gatcaaccgc acccggccct ctgccggcca ccgatccagg tggatgcgtc
     2701 gcgccgcatg ggcgagtgcg atcgatgagg cgtccacccc gagaatctcg gtgaactggc
     2761 gatcttccac gagctggagg atcagcttgc cctcgccgca gccgagatcg accacgcgct
     2821 ggactccccg atcgcgcagc accgcggcga cggccttgag ccgctcctca tggaggctga
     2881 tcggccgctc gacggtctct tcgaggttcg gagcggagct gtcctgcccg tccggatcgg
     2941 tcccgtcgct gattgccatg cgctcgatcg cgtgacgaac cagcgcgcgg cggtatcgca
     3001 ggtagcgatc ggcgatcagc ttgcgctcgg ggtgctggtc gagccagccc tcgccgcggc
     3061 gcagtagctt gtcgagctcg tcctcgccga accagtagtg cttgtcgtca tcgagcaccg
     3121 ggatcagcac ggtgagttgg cgcaagacat cctgcaggcg ggccgtgacg ccgagggtca
     3181 cgcagtaggc cgggccggat ccccactccg ggaaccgctc gtcgagcggg agctgcaccg
     3241 cttcgatacg gtcataacca agcggctcga acaggcgccg cagaaccgcc tcgccgcccc
     3301 ggcagcggat ggcactcagc ccaatctcga gcggaatcgg cgtcagagcg agatcggccc
     3361 ggtcccggga ttgcccgatg agcgagctcc ggtacacatc gccgattgcg acgctcagca
     3421 gcgacgacgc gacgtatggg cggtcgttga cgtactgggc gagggcaaac cgggctgagc
     3481 caggacctcg ccggacgagc cggatggggt cgacctcgac ctggagcgcg gccgtacacc
     3541 gggagtccgt ggcctcgggg taataaacgt gcgcctcgcc aaacgacaag ctgaacgact
     3601 ggcaccgacc cgggtatttg tgcagcaggt agccgagatc cgtcgccggt tgatgggttg
     3661 tcgaaatcgt caacagcatt gcttcatcca catcctgccg caggcagctg ccgacagcag
     3721 gtgtttcatc gtaccggcac gggtggcacg cgggtctggt ccgacagcga tcctccagca
     3781 tacacgggaa tctcgcgtgt aagagctcag gctccaatgc cgatgaagcc caggatgagg
     3841 atgacgagga cgatacctgt aatcgcgacg aagatccggt cccgctgcaa tatgaagagg
     3901 acgaacgtca tcgcgactcg ggtcagcggc gtcaggatca ggatcagcaa gccgagcagg
     3961 atgatcgaag tcgcttcgag gttgccgagc ccctccacga tcgagtgcca actcacgtcg
     4021 atcgactgac caccatcggc cgtcatttgg tccagcgtcg tgggccgtcc ggcatcagga
     4081 ccgttgacca ggtagaga
//
LOCUS       DATAOZ010000003         8251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7894237, whole genome shotgun sequence.
ACCESSION   DATAOZ010000003 DATAOZ010000000
VERSION     DATAOZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7894237"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VHR64_00035"
     CDS             complement(<1..443)
                     /locus_tag="VHR64_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEX3301241.1"
                     /translation="MSSITNTIEPATVTEVTLPINGMTCASCVRRVEKALGKTAGVAS
                     ANVNLATEKATVSYDPAIADIDSLRAAVEKAGYGVGTIAVAAPPSAPAPQPITGEVSL
                     PIEGMTCASCVRRVERSLTKVPGVESANVNLATEQATVAFDPAVA"
     gene            578..787
                     /locus_tag="VHR64_00040"
     CDS             578..787
                     /locus_tag="VHR64_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX3301242.1"
                     /translation="MTTITVKAPDISCGNCVAHVEKDVSALEGVQSVKAEVDSKLVTI
                     TFDDAKLSREQIAEAMDEAGYPIAE"
     gene            867..1256
                     /locus_tag="VHR64_00045"
     CDS             867..1256
                     /locus_tag="VHR64_00045"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX3301243.1"
                     /translation="MAVESGAVDQLVAALEHPLWAEIERLRAIILDANTGITEQIKWK
                     APSFCYRGDDRVTMKLYPASQIQLIFHRGARVKDTADFTFEDNSGLLKWAAKDRGVAT
                     LRNMAEIDANAAALTDLVNRWIIATSE"
     gene            complement(1277..1735)
                     /locus_tag="VHR64_00050"
     CDS             complement(1277..1735)
                     /locus_tag="VHR64_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX3301244.1"
                     /translation="MDDPPHSRIRVLLDANVLISHLLGGGAESTIGQTFRIAFGWRFD
                     LVVPADLLDELQNCRMKPYLAEKITESQVNRLVDDLEFMGTIVHPVRMGHSRILRDRG
                     DDYLLSTSVLNDVEIIVSGDKHLLNLRGILESPRIMSPADFVAEFGDVDR"
     gene            complement(1784..2113)
                     /locus_tag="VHR64_00055"
     CDS             complement(1784..2113)
                     /locus_tag="VHR64_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX3301245.1"
                     /translation="MTKTVTATEAKNRLGALLKEISQSNETVLIENRGTTQAVLISED
                     VYRSFLVLEWEQRRAEAIRRLEEIEAEVGARGDELTEEEAMELAVQAVREVRAEKAIE
                     QLKEDRQ"
     gene            2273..3895
                     /locus_tag="VHR64_00060"
     CDS             2273..3895
                     /locus_tag="VHR64_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX3301246.1"
                     /translation="MRLLRLALASCLVSGLITIAVITPAAAALTCVFEGTAYVATVNG
                     SGVITGTAGDDVIRGSNVADQISGLGGNDIICGRGGADTIDGGDGEDRITGDSNTDVI
                     KGGAGSDFIYGGTYDDRIDGGADSDLIAGDDGADMLVGGDGDDTLYGFAGNDRLYGQN
                     GADTIWGGAGDDYLSGSNSDDTLHGEDGNDRIAAGSSVSGETITGGPGNDSIAGGSAA
                     DSITGGIGDDVIDAAGGDDVIIADDGNDQVDAGDGNDQIVGGPGDDVLNGNLGNDRIS
                     DAAGANQIFGEDGNDILVGGTDADTISGGPGVDSIFGGDGPDSITGGDDNDRLYGEAG
                     NDSISGGAGQDTLSGEDGDDAMAGGTEDDQIFGGLGNDAIGGNEGDDLIIGGEGDDAI
                     TGESGLDTISGEEGDDKIDGGDDPDTIYGNAGNDSLRGGAGNDTIVGSSGNDLAYGDD
                     GDDQISLNDGADIGNGGFGNDAIYGGAGLDKLYGADGNDTLAGETGDGNRLYGQAGDD
                     SFLGAGGTNDYCSGSSGIDTTDGECETSLGIP"
     gene            4005..4238
                     /locus_tag="VHR64_00065"
     CDS             4005..4238
                     /locus_tag="VHR64_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301247.1"
                     /translation="MTRLFRWTVDLDALTDEAYAFVTDPRPSRTERPVIQELHEINPE
                     SVDVLESMLIDGTEEREDVAEYIGAVFGPFAAP"
     gene            complement(4311..5438)
                     /locus_tag="VHR64_00070"
     CDS             complement(4311..5438)
                     /locus_tag="VHR64_00070"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3301248.1"
                     /translation="MVTASEVSTTLGLVDLSLIETEFRSQLSVARLFPGAALAVYRHG
                     ELALDLVGGYADTQSGELVHAGSLFPLFSGTKPFGAVALWQQIERGKLALDDRVADYW
                     PAFGANGKGEVLVRHILSHRGGFPTTPAELEVRLWGDTEIVNRAIETMPLSHQPGAVP
                     AYHFVTQHWVIAELIRRVDGRAFDDYLADEITRPLGMWDTFVGVPVTREGDVVKLHAT
                     DGADEMSLAALRETRLVPFHQMVVPGASGVSTARDMARFYAAIAAGGGLGGVTILKPA
                     TVDRMMQIEIDGDIDVTFDAPVRRGLGFELGGLADPRRHWPGATSTVQTFWHGGMGSS
                     VSWADRDTGLAMAFLSNGIRRDAAGAIGRRDLSDLVRSTCR"
     gene            complement(5451..7019)
                     /locus_tag="VHR64_00075"
     CDS             complement(5451..7019)
                     /locus_tag="VHR64_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3301249.1"
                     /translation="MIIRSPHPDIAIPDIALTPFILRHAERLAPKPALIDGVSGHTMT
                     YQEIARAIRGTAAGLAERGFRAGDVFAIYAPNCIEYAVVFHAVSWLGGVTTTVNPAYT
                     ADELEHQLKDSDAIRLLTTPELMAKAAPAAFGAGISEIYVVGEAEGAIPFSDVSKSDG
                     DLPAAIVDTAKDTIALLYSSGTTGLPKGVMLTHQNLVANIEQTAAVGVYGEDDVVLCL
                     LPIFHMFGLQVLLNLGLASGATLVLLPRFEIESFLSAVERYGVTYAALVPPIVLILST
                     HEAVDRYDVTRLQRITCGAAPLSATTSRACANRLGCAVEQGYGLTETSPVTHVTVPGR
                     NVEGKVGPLAPNTEAKIVDLVSGESLGPNSPGELWVRGPQIMKGYLNRPEATAAMIDA
                     DGWLHTGDIACVDENGSFSIVDRLKELIKYKAYQVAPAELEAVLLTHPAVADVAVIPS
                     PDLETGEVPKAFVVLRGEASAEELMAFVADRVAPHKKVRRVEFIDCIPKSPTGKILRR
                     VLVEQERLALVATN"
     gene            complement(7218..>8251)
                     /locus_tag="VHR64_00080"
     CDS             complement(7218..>8251)
                     /locus_tag="VHR64_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3301250.1"
                     /translation="GEGKNWIERALAAGPADRWSYGSALLIGGWLALQLGDYASAERM
                     VKEGTGLAQELADQHWRGQILTVTGLVLEDRGRIEEAVGRYEEALAVYRSIGDIVWPA
                     YVLNALGLAAYRQGDLERAVPYFRESLAEFREGGNSFGEGLVLMNLGRVAKRRGEYAE
                     AHRLIVGSLALRWAQGDRPGILSCLRGLATLALLTGEHAHAARLLGAAEGLRSEIAAP
                     EPRQHDRYDRAITQARSALGDDAFAAAKKTGMETPLPEIVAGILNGEGPDTGRAASPD
                     QAKRSGLTSREIEVLRLIRDGLSNREIGERLFVSERTAQTHVQHILDKLDVGTRAEAA
                     ADAVERGLI"
BASE COUNT         1587 a         2710 c         2510 g         1444 t
ORIGIN      
        1 tcggccacag cgggatcgaa tgcaacagtc gcctgctcgg tggcgaggtt gacgttcgcg
       61 gactcaacgc ccggaacctt cgtcagggac cgctcgaccc ggcgcacgca ggaggcgcag
      121 gtcatgccct cgatcggcag cgagacctcg ccggtgatcg gttgcggggc cggcgcagac
      181 ggaggcgcgg cgacggctat cgtacccaca ccatagcccg ccttctcgac ggccgcgcgc
      241 aaagaatcga tatcggcgat ggccgggtcg taactgaccg tcgccttctc ggtcgccagg
      301 ttgacgttgg cggaggcgac gccggccgtc ttgccgagcg ccttctcgac acggcggacg
      361 cacgacgcgc aggtcatacc gttaatagga agcgtgacct cggtcacggt cgccggttcg
      421 attgtgttgg tgatgctgct cattccgaat ccggtcgtat cacgtgtatc aatgataccg
      481 cctcggggta tcttgacatg atgatacccc ctccccgtat aatgatcaag ggccggacag
      541 atacggccgc gatgaattgg tgttgaagga gtcagatgtg acgacgatta cggtgaaggc
      601 gcccgatatt tcgtgtggca attgcgtggc gcatgtcgag aaggatgtca gcgcgctcga
      661 gggcgtgcag tcggtcaagg ccgaggtcga ttccaaactg gtcacgatca cgttcgacga
      721 tgcgaagttg agccgcgagc agatcgcgga ggcaatggac gaggccggct acccgatcgc
      781 cgagtaggta gacgtcagac ttcagacatc agaatgggcg ggcggagccg gttgcctccg
      841 cccgtttcac atctggggag gatcgagtgg cagttgagtc gggtgccgtc gatcagctcg
      901 tggcggcgct ggagcatccg ctctgggccg agatcgagcg gctgcgggcg atcatcctcg
      961 acgcgaacac cgggattacc gagcagatca agtggaaagc gcccagtttt tgctaccggg
     1021 gcgacgaccg cgtcacgatg aagctctacc ctgcgagcca aatccagctc atcttccacc
     1081 gcggcgccag ggtaaaggac actgccgatt tcacgttcga ggacaacagc ggcctgctga
     1141 aatgggccgc caaagatcgc ggggttgcga cgctccggaa catggccgag atcgacgcga
     1201 atgcagccgc gctcaccgac ctcgtcaacc gctggatcat cgccaccagc gagtaaaaca
     1261 tgccgtcggg cacgtgctac cgatcaacat ccccaaactc cgcgacgaaa tccgccggcg
     1321 acatgatccg cggcgattcg agaattcccc gcaagttcaa caggtgcttg tcgcccgaca
     1381 cgatgatctc gacgtcatta agcaccgatg tggacagcaa gtaatcatct ccgcgatctc
     1441 taaggatgcg agagtgaccc atgcggaccg gatgaacgat cgtgcccatg aactcgagat
     1501 cgtcaaccaa acgattgact tgagattcag taattttttc agcgagatac ggtttcatgc
     1561 ggcagttctg caattcgtca agaagatcgg cgggaacaac caaatcaaac cgccatccaa
     1621 aggcaatacg aaaagtctgt ccaatcgtgc tctccgcccc accgccaaga agatgactga
     1681 tgagcacgtt ggcgtcgagc agtactcgaa tccgggaatg aggcggatca tccatggcga
     1741 agcacgactt ttcgcaggtg gaacctcagt ccaaccgacg acctcactgc cgatcctcct
     1801 taagttgttc gatcgctttt tcagcacgga cctcacgcac agcctgaacc gcgagttcca
     1861 tggcctcctc ttcagtgagt tcatcgcccc gtgcgcccac ctcggcctca atttcttcca
     1921 gccgtctgat tgcttccgct cgccgctgtt cccattcaag gacaaggaac gaccggtaca
     1981 cgtcctccga aatcaggacc gcttgggtgg tccctctgtt ttcgatcagg acggtctcat
     2041 tcgactgcga tatctctttt aacagggccc cgagacgatt cttggcctca gttgcggtta
     2101 cagtcttcgt catgccactt tccttgtcca tcgtgatagc taatctagag tctgtcatgt
     2161 tagcgttttg caccgacatc atcaatagct gtctctccgg ctatcaatcc gctgtgctag
     2221 gcttcgctcg tcccgcgctt tgtgtccgct tgtcataatt ggagggttcc cgatgcggtt
     2281 gcttcgcctt gccctggctt cctgcctcgt ctccgggctg atcaccatcg ccgtgatcac
     2341 ccccgccgca gccgccctga cctgtgtctt cgaaggcact gcctacgtcg ccaccgtcaa
     2401 cggatccggc gtcatcaccg gcaccgcggg cgatgacgtc atccgcggct cgaacgtggc
     2461 tgaccagatt tccggcctcg gcggcaacga catcatctgc ggtcgcggcg gcgcggacac
     2521 gatcgacggt ggcgacggtg aggatcgcat caccggcgac tccaataccg acgtcatcaa
     2581 gggcggggcc ggatccgact tcatctacgg cggcacctac gacgaccgca tcgatggcgg
     2641 ggccgacagc gacctgatcg ccggggatga cggcgccgac atgctcgtcg gcggcgatgg
     2701 cgatgacacg ctctatggat tcgccggcaa cgatcgtctc tacggccaaa acggcgccga
     2761 tacgatctgg ggcggggccg gcgacgacta cctctcgggc agcaacagcg acgacaccct
     2821 ccacggcgag gacggcaacg accggatcgc cgccggcagc tcggtcagcg gagagacgat
     2881 caccggcggt cccggcaacg actccatcgc cggcggatca gcggccgaca gcatcaccgg
     2941 cggcatcggc gacgatgtga tcgacgcggc cggaggggac gatgtcatca tcgccgacga
     3001 tggcaatgat caggtcgacg ccggcgatgg caacgaccag atcgtcggcg gcccgggcga
     3061 cgacgtcctc aacggcaacc tcggcaacga ccggatcagc gacgcggccg gggcgaacca
     3121 gatcttcggc gaggacggca acgatatcct cgtcggtggc acggacgccg ataccatctc
     3181 tggcggaccc ggcgtcgact cgatcttcgg cggtgacggt cccgactcga tcacaggtgg
     3241 ggacgacaac gaccgcctct acggcgaggc cggcaatgac tcgatcagcg gtggcgccgg
     3301 gcaggacacg ctcagcggtg aggacggtga tgatgccatg gcgggcggaa ccgaggacga
     3361 ccagatcttc ggcggcctcg gcaacgatgc gatcggcggg aatgaaggcg acgacctgat
     3421 catcggcggc gagggcgacg acgcgatcac cggcgaatcc ggtctcgaca cgatctccgg
     3481 cgaggaaggc gacgacaaga tcgacggcgg agatgatccg gacacgatct acggcaatgc
     3541 cggcaatgat tcgctccgcg gaggcgcagg caacgacacg atcgtcggat cttccggcaa
     3601 cgatctcgcc tacggcgacg acggcgacga ccagatctcg ctcaacgacg gcgccgatat
     3661 cggcaacggt ggattcggca acgacgccat ctacggtggc gccgggctcg acaagctcta
     3721 cggcgcggac ggcaacgata cgctcgctgg cgaaaccggc gacggcaacc ggctctacgg
     3781 ccaggcgggc gacgattcct tcctcggcgc cggcggcacc aacgattact gctccggcag
     3841 cagcggcatc gacacaaccg acggcgagtg cgagacctcc ctcggcatcc cctgagccat
     3901 gtgcgccggg ttcatcggtt cgtcatgcgg gaggcgcata atatagagga agcacggcgc
     3961 tggaacggcc attctgcccg ccgcgccacc aaaggaggcg caccatgacc cgcttgttcc
     4021 gctggacggt cgatctcgac gccctgaccg acgaggccta tgccttcgtg actgatccgc
     4081 gcccctcccg taccgagcgg ccggtgattc aggagctgca cgagatcaac cccgaatccg
     4141 tcgatgtgct cgaatcgatg ttgattgacg gtaccgaaga gcgcgaggat gtcgccgaat
     4201 acatcggcgc ggtcttcggc cccttcgccg ccccgtaacc ggtcgtagaa ctgataacgt
     4261 agttccgggc ggctcgcccg gctatggtga cgcggcgcgt gcgtcctgat ctatcgacag
     4321 gtcgatcgca ccaaatccga gagatcgcgc cgaccgatcg cacctgccgc atcccggcgg
     4381 attccattcg agaggaacgc catcgccaac ccggtgtcac gatccgccca actgacggat
     4441 gagcccatcc cgccgtgcca gaacgtctgc acggtgctgg tcgcgcccgg ccaatggcgc
     4501 cggggatcgg caagaccacc gagctcgaat cccagtccgc gacgaaccgg ggcatcgaac
     4561 gtgacgtcga tgtcgccatc gatttcgatc tgcatcatgc ggtcgacggt ggccggcttc
     4621 aggatggtca cgccgccaag cccgccgccg gctgctatcg cggcatagaa ccgggccatg
     4681 tcccgggcgg tcgacacacc actggcgccg ggaacgacca tctggtgaaa cggtaccaat
     4741 cgcgtctcgc gaagggctgc gaggctcatc tcgtcggccc catccgtggc atgcaacttc
     4801 accacatcac cctcgcgcgt gactggcaca ccaacgaagg tgtcccacat tccgagcggg
     4861 cgcgtgatct catccgccag gtaatcatcg aaggcacggc catccactct ccgtatcagc
     4921 tcggcgataa cccagtgctg cgtgacaaag tgatatgccg ggacagctcc cggctggtgg
     4981 ctcagcggca tggtctcgat cgcccgattc acgatctctg tgtcgcccca caacctcacc
     5041 tcgagttcgg caggcgttgt cgggaatccg ccgcggtggg acagaatgtg ccgaacgagc
     5101 acctccccct tgccattggc accaaacgcc ggccagtaat cggccacgcg atcatcaagc
     5161 gccagcttgc cgcgctcgat ctgctgccaa agggccaccg ctccgaacgg ctttgtcccc
     5221 gagaagagcg ggaagagcga gccggcgtgc acgagctcac cgctttgcgt gtcggcatac
     5281 ccaccgacca gatcaagcgc cagctcgccg tgccggtaga ccgccagcgc cgcgccggga
     5341 aacaatcgcg cgacgcttag ctgcgagcga aactccgttt cgatgaggga aaggtccacc
     5401 aggccgagag ttgtggaaac ttccgatgcc gtgaccacgc gattgccctt tcagttcgtc
     5461 gccaccaggg ccaatcgctc ctgctcgaca agcacccggc gcaggatctt gccggtcggc
     5521 gatttgggaa tgcaatcgat aaactcgacc cgccgcacct tcttgtgcgg agcaactcga
     5581 tcggcgacga acgccatcag ctcctccgcg ctggcctcgc cgcgcaagac gacgaatgcc
     5641 ttcgggacct cgcccgtttc gagatccggg ctgggaatca cggcgacatc cgcgacagcc
     5701 gggtgggtaa gcagcacagc ctcgagctcg gccggcgcca cctggtaggc cttgtacttg
     5761 atcagctctt tgagacggtc gacgatcgag aacgacccgt tctcatcgac gcaggcaatg
     5821 tcgccggtat gcaaccagcc gtccgcatcg atcatggccg cggtcgcctc tggccggttg
     5881 agatagccct tcatgatttg cgggccgcgt acccacagct cgccgggact gttcgggccg
     5941 agcgactcgc cggaaacaag atcgacgatt ttcgcctccg tgttcggagc cagcggaccg
     6001 accttcccct caacgttccg acccgggacc gtgacatggg taaccggtga ggtttcggtc
     6061 agtccgtagc cctgctcgac cgcgcagcca agccgattag cacaggcgcg actcgttgtc
     6121 gcactcagcg gggcggcgcc gcaggtaatg cgctggagcc gcgtcacgtc gtagcggtca
     6181 accgcttcat gcgtcgacag gatcaggaca atcggtggca ccagcgcggc ataggtcacg
     6241 ccgtaccgct caacggccga gaggaacgat tcaatctcga agcgcggcag cagcaccaac
     6301 gttgcccccg aggcgaggcc gaggttgagc agtacctgca ggccaaacat gtggaaaatg
     6361 ggaagcaggc agagaacaac gtcgtcctcg ccatatacac cgacggccgc tgtctgctcg
     6421 atattggcca ccaggttctg atgggtgagc atcacgccct tggggagccc ggtcgttccg
     6481 ctcgaataga gcagcgcgat cgtgtccttc gcggtgtcga cgatcgccgc cggcaaatcg
     6541 ccgtcgctct tcgacacgtc tgaaaacgga atcgcgccct ccgcctcacc gacaacgtag
     6601 atctcggaaa tccccgcccc gaacgcggcc ggagctgcct ttgccatcag ctccggtgtc
     6661 gtcagcaaac ggatcgcgtc cgaatccttg agctggtgct ccagctcatc ggcggtgtag
     6721 gccggattga ccgtcgtggt cactccgccg agccatgaga ccgcgtggaa cacaaccgcg
     6781 tactcgatgc agttcggggc gtagatggca aacacatcgc ccgcgcggaa tccacgctcg
     6841 gcaagaccag ctgccgtgcc gcgaattgcc cgggcaatct cctggtacgt catcgtgtgc
     6901 ccgctcaccc catcgatgag tgccggcttc ggagccaatc gctcggcatg gcggaggatg
     6961 aacggcgtca gggcgatatc agggattgcg atatcagggt gcggactgcg gatgatcatg
     7021 gaccgcttcc tttcgacgtc cgaaggctcc cgcgactcct cgcctgtggg gagccggtac
     7081 gtcttcgcca aaccgcatcg aactgatatc agtctgctga ccgtgcgttg ccctgtcgtc
     7141 cgctgatctg ccgatattcg cctacgcgcc cagctacgct tggcatacgt atacgtaggc
     7201 ggccggcgta ccgaaaccta gatcagaccg cgctcgaccg cgtccgcggc agcctccgcc
     7261 cgcgtcccga cgtcgagctt gtcgaggatg tgctggacgt gtgtctgggc agtccgctcg
     7321 ctgacgaaca ggcgctcgcc gatttcgcgg ttggacagac cgtcgcggat gagccgcaag
     7381 acttcgattt ctcgcgatgt gagaccgctc cgctttgcct ggtccggaga agcggctctc
     7441 ccggtatcgg gaccctcgcc attgaggatt ccggcgacga tctcgggaag gggcgtctcc
     7501 attccggtct tctttgctgc ggcgaaggca tcatcgccca gggctgatcg agcctgggta
     7561 atcgcgcggt cgtagcgatc gtgctgtcga ggttccggag cagcgatctc gctgcgcaac
     7621 ccctccgccg ctccaagtag ccgcgcggca tgggcgtgtt ctccggtcaa cagggcaagc
     7681 gtcgccagcc cccgaaggca gctgaggatc cccggtctat ccccttgcgc ccagcgcagg
     7741 gcaaggctcc cgacgatcaa ccggtgtgct tcggcgtact ccccgcgtct cttggcaacc
     7801 cggccgagat tcatgaggac gaggccctcg ccgaatgagt ttccgccctc acgaaattcg
     7861 gccagggact cgcggaaata gggcaccgcc cgctccagat ccccctgacg gtaggcggcc
     7921 aatccgaggg cattgagcac gtaggccggc cagacgatgt ctccaatgct ccggtaaacc
     7981 gccagcgcct cctcgtatcg cccaaccgcc tcttcgatcc ggcccctgtc ttcgagcacg
     8041 agtcccgtca cggtcaggat ctgcccgcgc cagtgctgat ctgccagttc ctgggccagg
     8101 ccggtgccct ccttgaccat gcgctcggca ctcgcgtagt cgcccagctg aagcgccagc
     8161 catcccccaa tgagcaacgc gctgccgtag ctccagcgat cagcgggacc ggccgcaagt
     8221 gctcgctcga tccagttttt gccctcgcca a
//
LOCUS       DATAOZ010000004         4871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4211827, whole genome shotgun sequence.
ACCESSION   DATAOZ010000004 DATAOZ010000000
VERSION     DATAOZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4211827"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1227)
                     /locus_tag="VHR64_00085"
     CDS             complement(76..1227)
                     /locus_tag="VHR64_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3301251.1"
                     /translation="MLADLRSEFLLDPSVTFLNHGSFGACPKPVFEDYQRWQLELERQ
                     PVEFLGRRYYDLMAESRADLGKYLNSDPNDLIYVDNATAGVNVVVRSMTLEPGDEIVT
                     TDHEYGACELTWQFLQESCGAVIKRAHIALPLSTHEEIVDALWAQVTERTKVIYLSHI
                     TSFSALIFPVEEICRRAREAGIFTVIDGAHAPGQIPLDMQAIGADCYAGNLHKWLCAP
                     KGAGFLYVRPEHQDMMMSVIVSWGWGEGRPEFGTTQFLRRNTWQGTRDPAAYLAVPAA
                     IQFQAEHDWPAIRGHCHELVVQARTRISNLTDLPPISPAGCDWFVQMATCPIRTKSIE
                     ELKTGLSDQYRVEIPCGEWQGMQYVRVSIQAYNTEADVDRLLDGLGRLL"
     gene            1307..2086
                     /locus_tag="VHR64_00090"
     CDS             1307..2086
                     /locus_tag="VHR64_00090"
                     /inference="COORDINATES: protein motif:HMM:NF009389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3301252.1"
                     /translation="MQQSQERPVAIVTGASRRIGIGAATCRALAGSGHDILFTHLQEY
                     DLDVSVGADAEGPRALAEELRGLGANVAEMHADLMDPATADAILARCTQQLGPPSVLV
                     NNAAYSTTQNWDELTPEEFDNHYLVNFRGSSLLSIAFAKQFTRGHRGRIVSMTTGQAL
                     GPMPTELAYAASKGAIEAFVKSFAYAVGPLGITVNAVNPGPVNTGWMTSELKQYLEPK
                     FALGRIGQPDDIGKVIAFLASPAADWITGQVINAEGGFTRD"
     gene            2219..4342
                     /locus_tag="VHR64_00095"
     CDS             2219..4342
                     /locus_tag="VHR64_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3301253.1"
                     /translation="MATASNGKTIWFKGACPHDCPDTCATLTEVDPATGRAVNFIGDP
                     DHPITDGWLCAKVRPYLSRVYSPDRVLYPLRRVGPKGSGQFERISWDAALSEIATRWT
                     AIIAEHGAEAILPYTYSGTLGTVELNVAASRFWGRLGSSQSVGDLCSGATRAAATATI
                     GGNLAPDPRHVLQSKMVLIWAHNPASTSPHFVPLMREAQRNGTKVVVIDPRRTKTARS
                     ADWHIQPLPGTDAALALAMINVIFDEGLHAEEWLEQHSVGWRQLRERAAEYPPDQAAA
                     VTGVDAKTIRDLAIEYATTKPSMIKVAQAINRHLHGGQTVRAIACLPAVAGQMGIRGG
                     GFFTSTSSHIRFNGRVMTRCDKFAEQREINMIQIGANLLGKISDPPIMSLFVFGANPV
                     AATPNSSETIQGLLREDLFTVVHDQFMTDTAEYADIVLPSVTQLERVDMHVPYGHMNL
                     QYNHKAIEPLGESVSNWDLMRMLATAMGFDEPWLHQTADEVIDELLTETARINPRFEG
                     VTLERLQREGTVPIFDEDYVPFADGTFPTPSGKMELWSQKFADAGLDPLPTWTPEFNP
                     DKPGGLIVLSGAPHHSISTSMGGDAELMRKEGDPHLVIHPEDAAERGIEHGQMIWVEN
                     ERGNCLLRAVVSTDVPRGVTMCPKGRWAKLSPGGRTLNWLISDAVSDIGMQATFHSTM
                     LWARPATPAEVGAAVDEPELALAGD"
     gene            4477..>4871
                     /locus_tag="VHR64_00100"
     CDS             4477..>4871
                     /locus_tag="VHR64_00100"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3301254.1"
                     /translation="MVGQSIALRARAGLFGDRNFRLIWSAATVSAFGYYVTDIAIPLL
                     AIEQLDVSSFQIGLIRVVQQLPNLFFGLFLGVLVDRARKRPLMITSDIVRAIVMLAIP
                     LAAFRDMLNLPIVLVVVFVVGAFNLLFDV"
BASE COUNT          837 a         1533 c         1660 g          841 t
ORIGIN      
        1 cccccccccg cgctacgttc gagatattgc ctttgcgagc gccattatcc cggagatttt
       61 cggtctgcgc actggttaga gcaggcggcc gagcccatcg agcaggcggt cgacgtcggc
      121 ctcggtgttg taggcctgga tcgagacgcg gacgtactgc atgccctgcc actcgccgca
      181 ggggatctcg acgcggtact ggtcggagag gccggtcttg agctcctcga tgctcttcgt
      241 gcggattggg caggtcgcca tctggacgaa ccagtcgcag ccggcaggcg agatcggtgg
      301 cagatcggtc aggttcgaga tgcgggtccg cgcctgcacg acgagctcat ggcaatgccc
      361 gcggatcgcc ggccagtcgt gctcggcctg gaactggatt gcggccggca ctgccaggta
      421 ggcggcggga tcgcgcgtgc cttgccaggt gttgcggcgg aggaactggg tcgtgccgaa
      481 ctcggggcgg ccctcgcccc agccccacga gacgatcacg gacatcatca tgtcctggtg
      541 ctcagggcgg acatagagga agccggcacc cttgggcgcg cagagccact tatgcaagtt
      601 gccggcgtag caatcggcgc cgatcgcctg catgtcgagc gggatctggc ccggggcatg
      661 ggcgccgtcg atcacggtga agatgccggc ctcccgggcg cggcggcaaa tctcctcgac
      721 cgggaagatc agggcgctga acgaggtgat atgcgagagg tagatgacct tcgtgcgctc
      781 ggtgacctgt gcccagagcg catcgacgat ctcctcgtgc gttgaaagcg gcagggcgat
      841 atgggcgcgc ttgatgaccg cgccgcacga ctcctggagg aactgccagg tcaactcgca
      901 ggcgccgtac tcgtggtcgg tggtgacgat ttcgtcgccc ggctcgaggg tcatcgacct
      961 gacgacgacg ttgactccag cggtcgcgtt gtcgacgtag atcaggtcgt tcgggtcgga
     1021 attcaggtat ttgccgaggt cggcgcggct ctcggccatg aggtcgtagt agcggcgacc
     1081 gaggaactcg accggctggc gctccagctc gagctgccag cgctggtagt cctcaaatac
     1141 cggcttcggg caggcgccga aggagccgtg gttcagaaac gttacagacg ggtcaagcag
     1201 gaattcggat cgaagatcag ccagcatcaa ttgcacccat tcccggtagg attcgcatgt
     1261 cgcggtcgtc agtgtatccg gggacgacgt aatccgggat agggaaatgc agcaatcaca
     1321 ggaacggccg gtcgcgatcg taaccggcgc cagccggcga atcgggatcg gcgcggcgac
     1381 gtgccgggcg ctcgccggga gcgggcacga catcctcttc acgcacttgc aggagtacga
     1441 cctggatgtc tcggtcggcg ccgatgctga agggccgcgg gcactggccg aggagctacg
     1501 cggtctcggc gcaaacgtgg ccgagatgca cgccgacctg atggatccgg cgacggccga
     1561 cgcgattctc gcgcggtgca cgcagcagct tggcccgccg tcggtgctgg tcaacaatgc
     1621 cgcatactca acgacccaga actgggacga gctgacgccg gaggagttcg acaaccacta
     1681 cctggtcaac ttccggggct cgtcgctcct gtcgatcgcc ttcgcgaagc agttcacgcg
     1741 cggacatcgc gggcggatcg tcagcatgac gaccggccaa gcgctcgggc cgatgccgac
     1801 tgagctggcg tacgcggcca gcaagggcgc gatcgaggcg ttcgtcaagt cgtttgcgta
     1861 tgccgtaggc ccgctgggga tcaccgtcaa cgccgtcaat cccggcccgg tcaacaccgg
     1921 ctggatgacc agcgagctga agcagtacct ggagccaaag ttcgcgctcg gccggatcgg
     1981 gcagccggac gacataggaa aggtgatcgc cttcctggcc agccccgccg ccgactggat
     2041 caccggtcaa gtcatcaacg ccgagggtgg gttcacgcgg gattaggcgg tcaatgaggc
     2101 gcgggtttgc cgtgggaagc ccctacaggt cgtttcagtt cacgactgct gctcgcctgc
     2161 tcgcctttct tctgatagtg tgcattggtg cgttcccttc ccatgtggag atgacgagat
     2221 ggcgacggca tccaacggca agacgatttg gttcaagggc gcgtgtccgc acgactgccc
     2281 cgatacctgc gcgacgctga ccgaggtcga tccggccacc ggacgggccg tcaatttcat
     2341 cggcgatccg gatcacccga tcaccgacgg ctggctctgc gccaaggtgc ggccgtattt
     2401 gagtcgcgtt tactcgccgg accgtgtcct ctacccgttg cgccgggtcg ggccgaaggg
     2461 ctcgggccag ttcgagcgca tttcgtggga tgccgcgcta tccgagatcg cgacccgctg
     2521 gacggcgatc attgccgagc acggggctga ggcgatcctg ccctacacct actccggcac
     2581 gctgggcacg gttgaactga acgtggccgc gagccgcttc tggggccggc tgggatcgag
     2641 ccagtcagtc ggagatcttt gctcgggcgc gacccgggcg gcggctaccg cgacaatcgg
     2701 cggcaatctc gcaccggatc cgcgtcacgt cctgcagagc aagatggtgc tgatctgggc
     2761 gcacaacccg gcttcgacca gcccccattt tgtgccgctg atgcgcgagg cgcagcggaa
     2821 tggcaccaag gtcgtggtga tcgatccgcg ccgaaccaag accgcgcgct cggccgattg
     2881 gcatatccag ccgctgccgg ggaccgacgc ggccctggcg ctggcgatga tcaacgtgat
     2941 cttcgacgag gggctgcacg ccgaggagtg gctggagcaa cactcggtcg gttggcgcca
     3001 gctgcgggag cgggcggcgg agtacccgcc cgaccaggct gccgccgtca ccggcgtcga
     3061 cgcaaagacg atccgcgatc tggcgatcga gtacgcgacg accaagccgt cgatgatcaa
     3121 ggtcgctcaa gcgatcaacc ggcacctgca cggcggccag acggtccgcg cgattgcctg
     3181 tttgccggcg gtggccgggc agatggggat caggggcggc gggttcttca cgtccacctc
     3241 gagccacatc cggttcaatg gccgggtgat gacgcgctgc gacaaatttg cggagcagcg
     3301 cgagatcaac atgatccaga tcggcgcgaa cctgctcggc aagatcagcg atccgccgat
     3361 catgtcgctc ttcgtcttcg gggcgaaccc ggtcgcggcc acacctaact cgtccgaaac
     3421 gattcagggg cttctgcgcg aggatctgtt caccgtcgtc cacgatcagt tcatgaccga
     3481 tacggccgag tacgcggaca tcgtgctgcc gtcggtgacc cagcttgagc gggtcgatat
     3541 gcacgtcccg tacgggcaca tgaaccttca gtacaaccac aaggcgatcg agccgctcgg
     3601 cgagtcggtc tcgaactggg acctgatgcg catgcttgcg accgcgatgg gctttgacga
     3661 gccgtggctg caccagaccg ccgacgaggt gatcgacgag ttgctgaccg agacggcgcg
     3721 gatcaacccg cgtttcgagg gcgtgacact cgagcggctg cagcgcgagg gtacggtccc
     3781 gatcttcgac gaggactacg tgcccttcgc cgacggcacg ttcccgacgc catccggcaa
     3841 gatggagctc tggagccaga agtttgccga tgccgggctc gatccgctgc cgacgtggac
     3901 gccggagttc aatccggaca agccgggcgg gctgatcgtg ctgtcgggcg cgccgcacca
     3961 ctcgatctcg accagcatgg gcggggacgc cgagctgatg cgcaaggagg gcgatccgca
     4021 cctggtgatc cacccggagg acgcggccga gcgcgggatc gagcacggcc agatgatctg
     4081 ggtcgagaac gagcgcggga actgcctgct cagggccgtc gtcagcaccg acgtgccgcg
     4141 cggcgtcacg atgtgcccga agggacgctg ggcgaagctg tcgccgggcg ggcgaacgct
     4201 caactggttg atcagcgatg cggtcagcga tatcggcatg caggcgacct tccacagcac
     4261 gatgctctgg gcccggccgg ccacacccgc ggaggtgggc gcggcggtcg acgagcctga
     4321 actggcgctg gccggagact agagcgatgg ccgtcaagcg ctgctggggc atgttccccc
     4381 aactgagatt cgtcctgatg cattagctgc cggggcatcg atcttccccg gcgggtcctg
     4441 cggacccatt ccattgcagt aggacagcag ttcaccatgg ttggacaatc gatagccttg
     4501 cgtgcgcgcg ccggcttgtt cggcgatcgc aactttcgcc tgatctggag cgcggcgacc
     4561 gtctccgcgt tcggctacta cgtcaccgac atcgcaatcc cgctgctggc gatcgagcag
     4621 ctcgatgtca gctcgtttca gatcggcctg atccgggtcg tgcagcagct gccgaacctg
     4681 ttcttcgggc tattcctcgg cgtcctggtc gaccgcgccc ggaagcggcc actgatgatc
     4741 acgtcggaca tcgtgcgggc gatcgtgatg ctggcaatcc cgctggcggc cttccgggac
     4801 atgctcaacc tgccgatcgt gctggtcgtc gtcttcgtgg tcggcgcatt caacctgctc
     4861 ttcgacgtca g
//
LOCUS       DATAOZ010000005         6090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7632381, whole genome shotgun sequence.
ACCESSION   DATAOZ010000005 DATAOZ010000000
VERSION     DATAOZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7632381"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VHR64_00105"
     CDS             <1..1114
                     /locus_tag="VHR64_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301255.1"
                     /translation="ATALYIILLDEYERWTGDTKLVRELEYEARSALKWIDVYADLRG
                     NGYVWYKRRNEETGLENQCWKDSWDSISFHDGTLPGFPRATCELQGYAYDAKLRGARL
                     ARLVWKDPVFAEKLESDATDLKRRFNRDFWVEKGEFYALALDPDGKQVDALASNNGHL
                     LWSGIVDKSRAKSVAQHLMGPRLFSGWGVRTLAEGEGRFNPIGYHVGTVWPFDNSFVA
                     WGLRRYGFKQEAAQIAAGILDAAEFFDGRLPEAFGGYRREVTKYPVQYPTACSPQAWS
                     TGAPLLLIRTMLGLEPMGDHLVVDPALPEGIGHVELLDIPGRWGRLDAFGRGRVHVEQ
                     RLGQRAQLGTVSKGTPNGGTNGRKRSERTTRARRAS"
     gene            complement(1199..3259)
                     /locus_tag="VHR64_00110"
     CDS             complement(1199..3259)
                     /locus_tag="VHR64_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX3301256.1"
                     /translation="MSTPITADRPLAMPPGPAAESILDRPHVHFGREICGDLESGLRR
                     EWLVTNGIGGYASATLPGILTRSYHGLLVAALEPPVARTVLVAGSSEWVGYDGKRYPL
                     STHEFGAGAISPDGFRYLESFRLEGALPVWTYAIADALIERRLWMHYGVNSTYVSYRL
                     LRGSLAADIEITPLVTYHTFHALVSGHDWSVNVDAMERAATIRAFDGAVPFHALSDRA
                     RFTPGGAWWWGFEHRAEASRGLGDHGDLYAIGTFRARLEPGETVAMLYTTEAMAELDA
                     AASLAVAQDRQRQLLAQAVAQKSDPIAQQLVLAADQFLVARPQPDNPSGRSVIAGYHW
                     FNDWGRDTMISLPGLTLATNRAKEAGSILRTFARYVSDGLLPNNFPDNAGIIPGYNTA
                     DATLWFVLAIRAYQEATGDEQLATDLLPALVQIIDRHIAGTRYRIGVDPADGLLRAGE
                     PGVQLTWMDAKVGSWVVTPRIGKPVEINALWYNALRTVSELLDGRGDRETATRYLALA
                     SQTRDSFQTRFWSRDRAHLADVVDSPDGDDWTLRPSQIFAISLPYPLLDGDDARYVLD
                     EVGRSLLTSYGLRSLAQDDPAYHGDYGGDQLRRDGCYHQGPVWSWLLGPFAEATYRLT
                     GDREAALDVLRPIGDHLRDAGLGSVSEIFEGDAPHVPNGCIAQAWGVAEVLRAWRLIE
                     KAAP"
     gene            complement(3353..6055)
                     /locus_tag="VHR64_00115"
     CDS             complement(3353..6055)
                     /locus_tag="VHR64_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301257.1"
                     /translation="MASVMDAPRFGEKIGSEWRTWGPYLSERQWGTVREDYSESGTAW
                     DFFPHDQARSRAYRWGEDGLLGISDDKQRLCFALALWNGIDPILKERLFGLTGSEGNH
                     GEDVKEYYYYLDATPTQSYLHGLYKYPQREFPYGDLLAENRRRNRDQPEYELVDTGIF
                     SENRYFDVDVEYAKVSPDDILVRITATNRGPDAASLHLIPTLWFRNTWSWGRDDRRPE
                     LSANAGNGGTAPAIRAVHHELGTLHLAYEGAPELLYTENETNAERLWGGANASPFVKD
                     GIHEAVVHGRTDAINPTMRGTKVGLHYLVNLAPGESESFRLRLSPNQQADPLTGADIA
                     IDDRKAEADELYAALAHGGLGEDEARIQRQALAGLIWSKQVYSFDVAQWLEGDPAGPP
                     PPEGRKHGRNSQWRHHNSSDVISMPDKWEYPWYAAWDLAFHCAAFGLVDPGFAKDQLL
                     LMLREWYMHPNGQLPAYEWAFGDVNPPVHIAATRLIYGHELRHTGEPDRRFLARVFHK
                     LLLNFTWWVNRKDEEGNNLFEGGFLGLDNISVIDRSMVLPEGLSLEQADGTAWMASYC
                     LNMGWAALELSMGDPAYEDLATKFLEHFMAIGGAMNGLSGEGTSLWDEEDGFYYDHVR
                     RSDGARMPLKLRSLVGLLPIFPAVALVGASLEEMRSKAPNFTNAVRWYADRHPELADL
                     LPLLSRSDGQESRMLTLVPEDRLRRILTRMFDPEEFLSDYGIRSISRHYLDHPYELQV
                     RDLNLTVKYEPAESSTGMFGGNSNWRGPIWFPTNILLIHGLRNLYDGYGDGFVVEYPT
                     GSGQRHTLAEIADDLSKRLTSVFLEDKNGNRPVFGGVELMQRDPNWHDHLLFYEYFHG
                     DNGAGIGASHQTGWTAMVANLLDRKASAVSPQRELATAGRHRSR"
BASE COUNT         1170 a         1899 c         1882 g         1139 t
ORIGIN      
        1 cgccaccgcg ctttacatca tcctgctcga cgaatacgag cgctggaccg gcgacaccaa
       61 actggtcaga gaactcgaat acgaggcgcg atcggcactc aagtggattg acgtctacgc
      121 agatctcagg ggcaatggtt atgtctggta caagcgccgc aacgaggaga ccgggctcga
      181 gaatcagtgc tggaaagact cctgggattc catctccttc catgacggca ccctccccgg
      241 cttcccgcgc gcgacctgcg agctgcaggg ttacgcatac gacgccaagc tccgcggcgc
      301 ccgcctggcg cgcctcgtct ggaaagatcc ggtattcgcc gagaagctcg agagcgatgc
      361 gacagatctg aagcgacgat tcaatcgcga cttctgggtc gagaagggcg agttctacgc
      421 cctcgccctg gatccggacg gaaagcaggt tgacgcgctg gcgtcgaaca acggacacct
      481 gctctggagt ggaatcgtcg acaagtcacg ggcgaaatcc gttgcccagc atttgatggg
      541 cccgcggctc ttctccggct ggggcgtgcg gacgctcgcc gaaggagaag gtcggttcaa
      601 tccaatcggc tatcacgtcg ggaccgtctg gccgttcgac aactcgttcg tcgcgtgggg
      661 cttgcgccgg tacggtttca agcaggaggc ggcccagatc gcagccggca tcctcgatgc
      721 cgccgagttc ttcgatggcc gccttccgga ggcgttcggc gggtaccggc gtgaggtcac
      781 gaaatacccg gtccaatacc caacggcatg cagtccgcag gcctggtcaa ccggcgctcc
      841 ccttctcctg attcggacca tgctcggcct ggagccgatg ggcgaccatc tggtggtgga
      901 tccggctctt ccagaaggca tcggccacgt cgagctcctg gatattcccg ggcgctgggg
      961 ccgactggac gccttcggac gaggtcgcgt ccacgtcgaa cagcgactgg ggcaacgagc
     1021 gcagctcggc accgtatcca aaggtacgcc gaatggtgga acgaacggcc ggaaacggag
     1081 cgaacgcacg acgcgcgccc gacgcgcgtc ctgacaccct ctgtccagac cggtccaaac
     1141 atcaatggcc aggggagttc ccttggccat tgatcattat tgacgcttgg agatcgagct
     1201 acggcgccgc tttttcaatc aagcgccaag ccctcaggac ttcggccaca ccccaggcct
     1261 gagcgatgca gccgttcgga acgtgcggag cgtcgccctc gaatatctcc gagacagagc
     1321 cgagaccggc atcgcgcaag tgatcgccga ttggtcgcaa gacgtcgagc gccgcttcgc
     1381 ggtcaccggt caggcggtag gtagcctccg cgaacggccc aagcaaccaa ctccagactg
     1441 gtccctggtg atagcagcca tcccgccgca actggtcacc gccgtagtcg ccgtggtagg
     1501 ccggatcatc ctgcgcgagt gaccggagac cgtaacttgt caacagagac cggccgactt
     1561 catcaagcac gtaccgcgca tcatcgccat cgagaagcgg gtatggaagc gaaatcgcga
     1621 aaatttgact cggacgaagc gtccagtcat cgccatctgg gctatcgaca acatccgcaa
     1681 gatgggcgcg atctcgtgac cagaagcgag tctggaaaga gtctcgcgtt tgactggcga
     1741 gcgccaagta tctcgttgcc gtctcccgat cgccccgccc atcaaggagt tcggaaacag
     1801 tgcgcagtgc gttgtaccag agtgcgttga tctcgaccgg ctttccgatc cggggcgtca
     1861 ccacccagct gccaaccttt gcgtccatcc acgtcaattg aacgcccggc tcgccagccc
     1921 gaagtaatcc gtcggccggg tcaacgccaa tcctgtaccg ggtcccggca atgtggcggt
     1981 caatgatctg gacgagcgcc ggcaggagat cggtcgcgag ctgttcatcg ccggtcgcct
     2041 cctggtacgc gcggattgcc agaacgaacc agagcgtcgc gtcggctgtg ttgtaacccg
     2101 gaatgatgcc ggcattgtcc gggaagttgt tcggcagcaa tccgtccgag acgtaccgtg
     2161 caaacgtccg taggatcgag cccgcttcct tcgctcgatt ggttgcaagc gttagaccgg
     2221 gcagactgat catcgtgtcg cggccccaat cgttgaacca gtgatatccg gcgatgacgc
     2281 tgcggccgga tgggttgtcg ggctggggcc gggccaccaa aaactggtcg gccgcgagca
     2341 caagctgctg tgcgattgga tccgattttt gcgcaacggc ttgtgccagc agttggcgct
     2401 ggcgatcttg cgcgacagcc agggacgccg ccgcatcgag ttccgccatc gcttcggtcg
     2461 tgtagagcat cgcgactgtc tcgccgggtt ccaggcgcgc ccggaacgtg ccgatggcgt
     2521 agaggtcgcc gtgatcgccg agtccacgac tggcctcggc ccgatgctcg aaaccccacc
     2581 accaggcccc gcccggggtg aacctggctc gatccgagag cgcgtgaaac ggcaccgcgc
     2641 cgtcgaacgc ccgaatcgtt gcggcccgct ccatcgcgtc gacgttgacg gaccagtcgt
     2701 gacccgagac gagggcatgg aacgtgtgat aggtgacgag cggcgtgatt tcgatgtcgg
     2761 cggccagact gccccgcaag agccggtagg agacgtaggt cgaattgacc ccgtagtgca
     2821 tccagagccg gcgctcaatg agggcatcgg cgattgcgta tgtccagacc ggcaacgctc
     2881 cctccaaccg gaacgattcg aggtagcgaa atccgtccgg agatatggca ccggcaccga
     2941 actcatgggt tgagagtgga taccgcttgc cgtcgtagcc gacccactcg ctcgagccgg
     3001 ccacgagcac ggtccgggca acgggaggct caagtgccgc cacgaggagg ccatggtatg
     3061 aacgagtgag gattcccggc agggtcgccg atgcgtaccc tccgatcccg ttcgtgacca
     3121 gccactccct ccgaagtccg gattcgagat cgccgcagat ttcgcgtcca aagtgcacat
     3181 gtggtcgatc gaggatggat tcggcggccg gaccaggggg catcgcgagc gggcggtccg
     3241 cagttattgg cgtactcatg gtcattttcc ccggaaagtt gaggacatcc ctgtcgaatg
     3301 acaagtggat aggcccgatt cgaacgagcc gaatcgggcc gggtcgagtc gattatctgg
     3361 agcggtgtcg gccggcagtt gccagctccc gttgaggcga gacggcactt gccttgcgat
     3421 cgagcaaatt cgcgaccatg gcagtccagc cggtctgatg actcgcaccg atcccggcgc
     3481 cgttgtcgcc gtggaagtac tcgtagaaga ggaggtgatc gtgccagttt gggtcgcgtt
     3541 gcatcagctc gacaccgcca aacacgggcc gattcccgtt cttgtcctcc agaaagactg
     3601 aagtcagccg cttcgagaga tcgtccgcaa tctcggcgag cgtatgccgc tgtcccgatc
     3661 ccgtcgggta ctcgacgacg aagccatctc catacccgtc gtacaggttg cgaagcccat
     3721 gaatgagcaa aatattcgtc gggaaccaga tcggtcctct ccagttggag ttcccaccga
     3781 acattccggt gctcgattcg gcgggctcgt acttgacggt caggttgaga tcccggacct
     3841 ggagctcgta cggatggtcg aggtagtgcc ggctgatcga cctgattccg taatcgctga
     3901 ggaattcctc cggatcaaac atccgggtca ggatccggcg gagacggtcc tccggaacca
     3961 gggtcagcat gcgactttcc tggccgtccg atcgggacag caacgggagc aggtccgcga
     4021 gctccggatg tcgatcggcg taccaccgca ccgcgttcgt gaagttcggc gccttcgatc
     4081 tcatctcctc aagggacgcg ccgacaagtg ctaccgccgg gaaaatcggg agcagtccga
     4141 ccagtgaccg gagtttcagc ggcatccgcg cgccgtcaga tcggcgcacg tggtcgtagt
     4201 agaagccgtc ctcctcgtcc cagaggcttg taccctctcc ggaaagccca ttcatggctc
     4261 cgccaatggc catgaagtgc tcgaggaact tcgtggccag atcttcgtac gctggatcgc
     4321 ccatcgacaa ctcgagagcg gcccacccca tgttcaggca gtagctcgcc atccaggctg
     4381 ttccgtcggc ttgttcgagg gacaagccct ccggcaggac catgctccgg tcgatgacgc
     4441 tgatgttgtc gagtcccagg aatccgccct cgaacaggtt gttcccttct tcgtccttgc
     4501 ggttgaccca ccaggtgaag ttgaggagca gcttgtggaa gacgcgcgcc aggaatcggc
     4561 gatccggctc gccggtgtgc cgaagctcgt gaccgtagat gagccgggtg gcggcgatgt
     4621 ggacgggcgg attgacgtca ccgaacgccc actcgtaggc cgggagttgg ccattcgggt
     4681 gcatgtacca ctcccgcagc atcagcagga gctggtcctt ggcgaaacca ggatcaacga
     4741 gtccgaatgc ggcgcagtgg aaggcaagat cccaggcggc gtaccacggg tactcccact
     4801 tgtccggcat cgagataaca tcgctgctgt tgtggtgacg ccactggctg ttgcgcccgt
     4861 gtttgcgtcc ctcgggcggc gggggaccgg ccggatctcc ctcgagccat tgcgcgacat
     4921 cgaaggagta gacctgcttg ctccagatca ggccggccag cgcctgtcgc tggatccgcg
     4981 cctcgtcctc gccaaggccg ccatgtgcaa gcgccgcgta caactcatca gcttcggcct
     5041 ttcggtcatc gatggcgatg tccgcgccgg tcaatggatc agcctgctgg ttcggagaga
     5101 gccggagccg aaacgactcc gattcacccg gcgccaaatt gaccaggtaa tgcagcccga
     5161 ccttggttcc tcgcattgtg ggattgatcg catcagttcg cccatggacg actgcctcgt
     5221 ggatgccgtc ttttacgaat ggcgaggcat ttgctccgcc ccaaagccgc tcggcgttgg
     5281 tctcgttctc ggtgtagagc agctcaggtg cgccctcata cgctaaatga agcgtcccga
     5341 gctcgtgatg aaccgcgcga atcgcgggtg ctgttccccc gttgcccgca ttggctgaga
     5401 gctcgggacg tcgatcgtct cgaccccatg accaggtatt tctgaaccaa agcgttggga
     5461 tcaggtgcag ggacgccgca tccggaccac ggttcgtggc ggtgattctg acgagaatgt
     5521 catccggcga aaccttggcg tactcgacgt cgacgtcgaa ataccggttc tcgctgaaga
     5581 tgccggtgtc aaccagctcg tactccggct gatcgcgatt gcggcgccgg ttctctgcca
     5641 ggagatcgcc atacgggaat tctcgctgcg gatacttgta gaggccgtga agataggact
     5701 gggttggcgt cgcatcgagg tagtagtaat actccttgac gtcctcaccg tggttccctt
     5761 cactaccggt cagcccgaac aggcgttcct tgagaatggg atcgatcccg ttccagaggg
     5821 cgagcgcgaa gcacaacctc tgcttgtcat cggaaattcc gaggagacca tcttcacccc
     5881 aacgatacgc gcgcgagcga gcctggtcat gcggaaagaa gtcccaggcc gtgccagatt
     5941 ccgagtagtc ttcccgcacc gttccccatt gccgctccga gagatatgga ccccaggtac
     6001 gccactccga accgatcttc tcgccgaatc gcggcgcatc cattaccgat gccattcgag
     6061 attctcccac tgtatcgatg ccaaatggcc
//
LOCUS       DATAOZ010000006         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_794246, whole genome shotgun sequence.
ACCESSION   DATAOZ010000006 DATAOZ010000000
VERSION     DATAOZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794246"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(409..705)
                     /locus_tag="VHR64_00120"
     CDS             complement(409..705)
                     /locus_tag="VHR64_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX3301258.1"
                     /translation="MIRVFLPPNLKIMARVDRELTFSIDGPVTQRTVLDAVEAEYPML
                     RGTIRDHVTQQRRAFIRFFACEQDLSHESPDALLPDPVATGKEPYLIVGAIAGG"
     gene            complement(702..1814)
                     /locus_tag="VHR64_00125"
     CDS             complement(702..1814)
                     /locus_tag="VHR64_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301259.1"
                     /translation="MSGVRVLVGTRKGAFILTSDEKREKWDVSGPHFAGWEIYHLKGS
                     PVNPDRIYASQSSSWFGQTMQRSDDGGQTWETVGNQFQYIGDPGTHMWYDGTQHPWEF
                     TRVWHLEPSLTDPDTVYAGVEDAALFKSTDGGQTWNELDGLRAAKGHLWQPGAGGMCL
                     HTILLDPTNPDRIYIAISAAGAFRTDDGGTTWTPINKGLKSNYIPEEQAEVGHCVHNL
                     AMHPSRPQTVFMQKHWDVMRTDDSGDMWHEVSGNLPTDFGFPISVHAHEPETIYVVPI
                     KSDSEHFPPDGKLRVYRSRTGGNEWEALTNGLPQQDCYVNVLRDSMAIDQLDSCGIYF
                     GTTGGQVYASNDSGDTWNAIVRDLPAVVSVEVQTLK"
     gene            complement(1927..2301)
                     /locus_tag="VHR64_00130"
     CDS             complement(1927..2301)
                     /locus_tag="VHR64_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3301260.1"
                     /translation="MINKIHSATVVVSDQDKALDFYVNTLGWQKVIDQPMGDGMRFIT
                     VTPVSGGTELSLGHSSWMGRGPGGETGISLLSDDIKGDYAALTAKGVRFKGEPEKMPW
                     GDTAAWFYDPDGNEFFLNGPGQ"
     gene            complement(2330..2842)
                     /locus_tag="VHR64_00135"
     CDS             complement(2330..2842)
                     /locus_tag="VHR64_00135"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX3301261.1"
                     /translation="MSTSNAAPKLEPRFDKRGQFRLAGFSRIMTFDQVGEIPQLWQKF
                     EGRWGEIPGQVDLVGYGVCFVPPDGNPGCFGYLAAIEIAADAELPDDMVEMTIPAHRY
                     AIVPHTGHVTEFGNTIRALSEEWLPQSGYAAPEPQPGAALFYERYAEDFNPNTGHGGM
                     EVWQPLATNH"
BASE COUNT          492 a          915 c          964 g          584 t
ORIGIN      
        1 agccctcgtc ggtcccgtag atcagcaaca tgtacttcac gcaatcgtct ccgtctgatg
       61 gttgggccag tcgaattgga atggcccaag ttgatatcga acctcatcgt agcgcggggt
      121 taccatgccc cgcttcgttc atccagacgc gtgttgtaag agttctgacc gatcccgtgg
      181 ttggttccta ctaacggcgt aggcgatacc gggttcgggc ggggcacggt gaccccgcgc
      241 tacgttcaag atgcgggtgc ctgccagcgg gccccgtgag agtccaggcg ggtacatccc
      301 cactccggct ctcccctcca gggaaaaagc aacctcgcca ctacgtcccc ggtctgcgca
      361 ttcggagccc cgccactacg agagtggcgg ggcgtgtatg gccgcatcct agccgccggc
      421 gatggcccct acgatcaggt agggctcctt gccggtggcg accgggtcgg gcagcagggc
      481 gtccggcgac tcgtgcgaga gatcctgctc gcaggcgaag aagcggatga aggcccgccg
      541 ctgctgcgtg acgtgatcac ggatcgtgcc gcgcagcatc gggtactcgg cctcgacggc
      601 atcgaggacc gtgcgttgcg tgaccggccc gtcgatcgag aacgtcagct ctcgatcgac
      661 gcgcgccatg atcttgagat tggggggtag aaaaacccga atcatttcag cgtctggacc
      721 tcgaccgaga caacggccgg cagatcgcga acgatcgcgt tccaggtatc gcccgagtcg
      781 ttcgaggcgt agacctggcc gccggtcgtg ccgaagtaga tgccgcacga gtcgagctgg
      841 tcaatggcca tggagtcgcg caggacgttg acgtagcaat cctgctgcgg gaggccgttg
      901 gtcagcgcct cccactcgtt gccgccggtg cggctgcgat agacgcgcag cttgccgtcc
      961 ggcgggaagt gctccgagtc gctcttgatc gggacgacgt agattgtctc cggctcatga
     1021 gcatggacgc tgatcgggaa gccgaagtcg gtcggcaggt tgccgctgac ctcgtgccac
     1081 atgtcgccac tgtcgtcggt ccgcatcacg tcccagtgct tctgcatgaa gacggtctgc
     1141 gggcgggacg gatgcatggc caggttgtgg acgcaatggc cgacctcggc ctgctcctcc
     1201 gggatgtagt tggacttgag gcccttgttg atcggcgtcc aggttgtgcc gccgtcatcg
     1261 gtgcggaagg cgccggccgc cgagatcgca atgtagatgc ggtccgggtt ggtcgggtcg
     1321 agcaggatgg tgtgcaggca catgccgccc gcgcccggct gccaaaggtg acccttggcc
     1381 gcgcgcagac cgtccagctc attccaggtc tggccgccgt cggtcgactt gaagagggca
     1441 gcatcctcga cgccggcgta gaccgtgtcg ggatcggtca gggagggctc gaggtgccag
     1501 acgcgggtga actcccacgg gtgctgggtg ccgtcgtacc acatgtgtgt gcccggatcg
     1561 ccgatgtact ggaactggtt gccgaccgtc tcccaggtct ggccgccgtc gtccgagcgc
     1621 tgcatggtct ggccaaacca gctgctggac tgcgaggcgt agatccgatc cgggttcacc
     1681 ggagagccct tgaggtggta gatctcccag cccgcgaagt gcggaccgga gacatcccac
     1741 ttctcgcgct tctcatccga tgtcaaaatg aacgcgccct tgcgcgtgcc gaccaatacg
     1801 cgtaccccgc tcatactgtt tccttcctca ccgttctgcc tcgacgaagg ttgttagcct
     1861 tcctatgtcc acgaccgggg cggccggtcg atggtccgat gtgattgcgg ggacgggtgc
     1921 ccaaggctat tggcctggtc cattcagaaa gaactcattg ccatccgggt cgtagaacca
     1981 ggcggccgtg tcaccccatg gcatcttctc cggctcgccc ttgaaacgga cgcccttcgc
     2041 ggtcagggcg gcgtaatcgc ccttgatgtc gtccgagagc agcgagatgc cagtctctcc
     2101 gccgggtccg cggcccatcc agctcgaatg gccgagagag agctcggtgc cgcccgaaac
     2161 cggcgtcacc gtgatgaatc gcatgccgtc acccatcggc tgatcgatca ccttctgcca
     2221 gccgagggtg ttgacgtaga agtcgagcgc cttgtcctgg tccgaaacga cgacggttgc
     2281 tgagtgaatc ttgttgatca tggctcctcc attggatggt gattagtggt tagtggttcg
     2341 ttgccaacgg ttgccagact tccattccgc cgtgtccggt gtttggattg aaatcttcgg
     2401 catagcgctc gtagaagaga gcggcgcccg gctgcggttc gggcgccgca tagccggact
     2461 gcgggagcca ctcctcggag agcgcgcgga tggtgttacc gaactctgtg acgtgaccgg
     2521 tgtgcgggac gatcgcgtac cggtgggcgg ggatcgtcat ctcgaccatg tcgtccggga
     2581 gctcggcgtc cgccgcgatc tcgatggcgg cgaggtagcc gaagcagccg gggttaccat
     2641 ccggaggcac gaagcagacg ccatacccaa cgagatcgac ctggcccgga atctcgcccc
     2701 atctcccttc gaacttttgc cagagctgcg gtatctcgcc gacctggtca aaggtcatga
     2761 tccggctgaa cccggctagc cggaactgtc cgcgcttgtc gaatctcggc tccaactttg
     2821 gtgcggcatt gcttgtgctc atgtcgaatc gcctcgtttc cagatccgcg attccgacta
     2881 ctgcagtgag gagagctgcg cgcggaggcg ttcctcctgc tcttgcagct cgggcgtgaa
     2941 ggcctcaccg aagtc
//
LOCUS       DATAOZ010000007         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7370422, whole genome shotgun sequence.
ACCESSION   DATAOZ010000007 DATAOZ010000000
VERSION     DATAOZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7370422"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VHR64_00140"
     CDS             <1..441
                     /locus_tag="VHR64_00140"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX3301262.1"
                     /translation="SLVPGTHEERRRVERGEPGDGSIIIIVATDAPLLPDQCRRIARR
                     ATIGLARVGGNGNNGSGDLFLAFSTANRGMFAGNTYEPFAVQSLQPDAMTPLFEATAD
                     ATEEAIVNALCMATTVTGVNGRTSHAVPLDRVRELMAKYNRLAS"
     gene            531..>1945
                     /locus_tag="VHR64_00145"
     CDS             531..>1945
                     /locus_tag="VHR64_00145"
                     /inference="COORDINATES: protein motif:HMM:NF006122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX3301263.1"
                     /translation="MAVSSTVDRSDVAATTQRMTGGDALAEMLIRNGVDTLFGLPGVQ
                     LDGFFNALWARKDKLRVIHTRHEQATAYMADGYARVSGREGVCVVVPGPGLLNAAAAL
                     STAYACNSPVLCVTGQIKSDLIDHGRGLLHEIHDQLGMIRHITKSAERAMTPGEIPGV
                     VSKSFAELRSGRMRPVEIEVPPDTLFAEDEVALVGPSAERVLITPDASRLEEAAQYLA
                     GAKNPLIYAGGGVIRGKAWTELVRLAELLQAPVVMSANGKGAISDRHYLAMNQVSELD
                     LRPKADVALIVGTRFLDNLSQPRPMNPETTLLRIDIDGEEIHRDVKPAVEMVGDAKAG
                     LSALIDLLEQMVGERPSRQAEYEQLRRRVTERINSVMPQAGFGLAIRNALPDDGIVVG
                     EMTQIAYWSNFAMPVYEPNTYITPGYQGTLGWGFCTSLGVKVGKPDSPVVSINGDGGF
                     GFALNELATMAQHEIPAIVLV"
BASE COUNT          337 a          641 c          632 g          335 t
ORIGIN      
        1 agcctggttc ctggcacgca cgaggagcgg agacgggtcg agcgaggcga gccgggtgac
       61 ggctcgatca tcatcatcgt tgccaccgac gcgcctctcc tgccggacca gtgccggcga
      121 attgcgcggc gagcgacgat cggcctggcc agggtcggcg gcaatggcaa caacggcagc
      181 ggcgacctct tcctcgcctt ctccaccgcc aatcgcggca tgttcgccgg caacacgtac
      241 gaaccgttcg cggttcagag tttgcaaccg gacgcgatga cgccgctctt cgaggcgacg
      301 gccgatgcga ccgaagaggc gatcgtcaac gcgctctgca tggctacgac ggtgactggc
      361 gtcaatggcc gcacgtcgca cgcagtgccg ctcgaccggg tccgcgagct gatggcgaag
      421 tacaaccggt tggcgtcctg aacagtttct gtcacccatc cgggcgttgt gcagcgggta
      481 caatgccctg tcgggaaatc gacccgtttt cgtttaagtg aggcgagttc atggcagtta
      541 gttccaccgt tgatcgatcc gatgtcgcag caacgacgca gcgcatgacc ggtggtgacg
      601 cactggctga aatgctgatc cgaaacggcg tcgataccct cttcggcctc ccgggcgtcc
      661 aactcgatgg cttcttcaat gccctctggg cgcgcaagga caagctccgc gtcattcaca
      721 cccggcacga acaagccacc gcctacatgg ccgatggcta tgcgcgggtc agcggtcgcg
      781 agggcgtctg tgtggtcgtt cctggtcccg gcttgctcaa tgctgccgcc gcgctctcga
      841 ccgcgtacgc ctgcaactcg ccggtcctct gcgtgaccgg gcagatcaag tccgacctga
      901 tcgaccatgg ccggggtctg ctccacgaga tccacgatca gctcggcatg atccgccata
      961 tcaccaagag cgccgagcgg gcgatgactc cgggcgagat cccgggtgtg gtcagcaagt
     1021 ccttcgctga actgcggtcc ggccggatgc gcccggtcga gatcgaggtt ccgccggaca
     1081 cgctcttcgc cgaggatgag gtcgcgctgg ttggtccgtc cgccgagcgg gtcctgatca
     1141 cgccggacgc gtcgcggctc gaagaggcgg cgcagtatct ggccggcgcg aagaatccgc
     1201 tgatctacgc cggcgggggc gtgatccgcg gcaaggcgtg gaccgagctg gtccggctgg
     1261 ctgagctgct gcaggcgccg gtcgtgatga gcgccaacgg caaaggcgcg atctcggatc
     1321 gacattacct cgccatgaac caggtctccg agctcgatct gcggccgaag gcggacgttg
     1381 ccctcatcgt cggcacccgc ttcctcgaca acctcagcca gccgcggccg atgaacccgg
     1441 agacgacact cctccgcatc gacatcgacg gggaggagat ccaccgcgat gtcaaacctg
     1501 ccgtcgagat ggtcggcgat gccaaggccg gcctatccgc gctgatcgat ctgctcgagc
     1561 agatggtcgg cgagcgcccc tcgcgccagg cggagtatga gcagctcagg cgccgggtca
     1621 ccgagcggat caacagcgtg atgccgcagg ccggcttcgg gctggcgatc cggaatgcgc
     1681 tgccggacga tggcatcgtt gtcggcgaga tgacccagat cgcctactgg tccaacttcg
     1741 cgatgccagt ctacgaaccg aacacgtaca tcactcctgg ctaccagggc accttgggct
     1801 ggggcttctg cacgtcactc ggcgtcaagg tcggcaagcc ggatagcccg gtcgtctcga
     1861 tcaacggcga cggcggcttc ggcttcgccc tcaacgagct ggcgaccatg gcccagcacg
     1921 agatcccggc gatcgttctc gtttt
//
LOCUS       DATAOZ010000008         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5003599, whole genome shotgun sequence.
ACCESSION   DATAOZ010000008 DATAOZ010000000
VERSION     DATAOZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5003599"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VHR64_00150"
     CDS             <1..342
                     /locus_tag="VHR64_00150"
                     /inference="COORDINATES: protein motif:HMM:NF040055.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HEX3301264.1"
                     /translation="GGGASYCLLLADETSPMGEERLQMMEATTDGFVLAEADLRMRGP
                     GDFLGTRQSGLPDLSMLRTGFDSRTLEQARRAAMRILDRDPDLSAPENLALRNRVRQF
                     WSSAVSDLAGA"
     gene            346..912
                     /gene="rsmD"
                     /locus_tag="VHR64_00155"
     CDS             346..912
                     /gene="rsmD"
                     /locus_tag="VHR64_00155"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX3301265.1"
                     /translation="MRVITGEAKGRKLKGPPSTATRPMQDKIKEALFSALESLDLDIT
                     RVLDLYAGTGGIGIEALSRGAEWADFVDQQKAACDVVRWNLAHTGLASRSAVHQMPVP
                     VFLRRKEQTYDLVILDPPYADPAIGETLEMLGDSQLVESGTIVALGHWPKFDSDSGTG
                     TLRLIKRRCHGDSCFSIFEVARGTATPE"
     gene            909..1394
                     /gene="coaD"
                     /locus_tag="VHR64_00160"
     CDS             909..1394
                     /gene="coaD"
                     /locus_tag="VHR64_00160"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX3301266.1"
                     /translation="MRTAIYPGSFDPITMGHIDVAKRAAHVFDHVIVAIYEQSEKPGA
                     LFTTAERAELARQSFCDHPEIEVATFSGLAVNYARERGATTIVRGLRAVSDFEYEFKL
                     AHMNAHLAPEIDVVCLMTSSRHSFVSSSLIREVASLGGNLEGLVPDHVLTALQKRFAR
                     P"
     gene            1391..2023
                     /locus_tag="VHR64_00165"
     CDS             1391..2023
                     /locus_tag="VHR64_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301267.1"
                     /translation="MTEDVDLQPGRMRETSREPLYKRRNVGMEPLEDDGLFPPSEPYE
                     PADAIDDTSVDIFYLLDRLEELVSLGKRVPFYGKVMVEENEFLALVDQLRIAVPNEIK
                     QAQRVIRERQTIIAESHEEAARILDAARKRAEYFVSQEGILNEARLKGEQILQDAHDK
                     RNRVMGEIDAYALEQFDKVEEAMRNGLALIDESLEETVAIMREARQNVGR"
     gene            2133..3080
                     /locus_tag="VHR64_00170"
     CDS             2133..3080
                     /locus_tag="VHR64_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX3301268.1"
                     /translation="MEQLLRRLSILGTIGMFTVLLMGATVTDTGSQTGCGRSWPLCHG
                     NFLPASVRESVIEFSHRAVTGVIGIVIVALAILSIRIRHKYPALLPLVPIMVGALLLQ
                     SGMGAWAVKYPQSAAVLALHFGFSLVAVAGVFLVMRVIDEQSSGRYVEKPPAPPLLKA
                     LFWASVVVVYVVAYSGAYIKHAHAEDGCRSWPLCDGQVWPGLSGDNGVIFMHRLAATG
                     SVLLLIGIAWYARRYREIRPDLYNVSLLAVLAVACQAVVGGIVAATNMQLISTLLHAG
                     LMTVLFLVLCEGCRLTIPQREPNRAWSPAQQAVAPQGAD"
     gene            3161..>3680
                     /locus_tag="VHR64_00175"
     CDS             3161..>3680
                     /locus_tag="VHR64_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX3301269.1"
                     /translation="MDPQFAGQVDLDNDSIVNVAALLREQTGATRSYRFQIDRFPLDD
                     DLDAVGVAGSLRLTRLSDAILASVSGTASVVLECQRCLEQFSLPVSVKFEEPFRIAYD
                     VRRGTAIESETEGIDERPEITENHELDFSEPMRQEIIVALPMRPVCGPDCPGPPIFAD
                     GDEEEGSGQFSAL"
BASE COUNT          641 a         1161 c         1195 g          683 t
ORIGIN      
        1 ggcggcggtg cgtcgtactg cctgctcctg gccgatgaga cgtcgccgat gggcgaggag
       61 cgcctccaga tgatggaggc gacgaccgac ggctttgtcc tggccgaggc cgatttacgg
      121 atgcgcggac cgggcgactt ccttggtacg cgccagagcg gtctgcccga cctctcgatg
      181 ctgcgcaccg gcttcgattc gcggaccctc gagcaggctc gccgggcggc catgcgcatc
      241 ctcgatcgtg atccggacct ctcggccccg gagaatcttg ccctgcgcaa ccgcgtccgc
      301 caattctggt catcggccgt ctccgacctg gcaggggcct gagcaatgcg cgttatcacc
      361 ggggaggcga agggccggaa actcaagggg ccgcccagca cggcgactcg tccgatgcag
      421 gacaagatca aagaggcgct cttctccgct ctcgaatcgc tggacctcga catcacgcgg
      481 gtgctcgatc tgtacgccgg caccggcgga atcgggattg aggcgctgtc ccgtggggcg
      541 gagtgggccg acttcgtcga tcagcagaag gcagcctgcg atgtcgtccg ctggaacctc
      601 gcgcacacgg gtttggccag ccggagtgcc gtccaccaga tgccggtacc cgtctttctg
      661 cgtcgcaagg agcagacgta tgacctcgtc atcctcgacc cgccgtatgc cgatcccgcc
      721 atcggcgaga cgctggagat gctcggcgac tcgcagttgg tagaatccgg cacgatcgtc
      781 gcgctcgggc actggccgaa attcgattcg gattccggga ccgggacact ccggctgatc
      841 aagcggagat gccacggcga cagttgcttt tcgatcttcg aggtggcgcg agggacggca
      901 acgccagaat gaggaccgca atttaccccg gcagtttcga cccgatcacc atgggccaca
      961 tcgacgtagc caaacgtgcc gcccatgtgt tcgaccacgt tatcgttgcg atttatgagc
     1021 agtccgagaa acccggcgcc ctgttcacga ccgcggagcg cgccgagctg gcccgtcagt
     1081 ccttctgcga tcacccggaa atcgaagtgg cgacctttag cggcctggct gtcaactacg
     1141 cccgcgagcg cggcgcgacg acgatcgtgc gtggacttcg ggccgtctca gatttcgagt
     1201 acgaattcaa gctcgcgcac atgaatgccc acctcgcgcc ggagatcgac gtcgtctgtc
     1261 tgatgacctc gtcgcggcac tccttcgtct cgtcgagcct gattcgtgaa gtggcctcgc
     1321 tgggtggtaa tctggaaggg ctcgtacctg accacgtcct gacggccttg cagaaacggt
     1381 tcgcacgccc atgactgaag acgttgacct ccagccaggc cgcatgcggg agacctcgcg
     1441 tgagccgctc tacaagcgcc ggaacgtcgg gatggagccg ttggaggacg atggcctctt
     1501 tccgcccagc gagccgtacg agcccgccga cgcgatcgac gatacgtcgg tagacatctt
     1561 ctacctgctc gatcgcctcg aggagctggt cagccttggc aagcgcgtgc cgttttacgg
     1621 caaggtcatg gtcgaggaga acgagttcct ggccttggtc gaccagctcc ggatcgccgt
     1681 cccgaacgag atcaaacagg cgcagcgcgt gatccgcgag cggcagacga tcatcgccga
     1741 gtcgcatgag gaggccgcac ggatcctcga tgctgcccgc aagcgcgccg agtacttcgt
     1801 ctcacaggaa ggcatcttga acgaggcgcg gctgaagggc gagcagatcc tgcaggacgc
     1861 gcacgacaag cgcaatcgcg tcatgggcga gattgatgcc tacgccctcg agcagttcga
     1921 caaggtcgag gaggccatgc gcaacggcct cgccctgatc gacgagtcgc tggaagaaac
     1981 tgtcgcgatt atgcgcgagg ctcgccagaa cgtcggtcga tagcgatcgc gcagccgtac
     2041 agcgttctgt gcgccccaac gagccattcg cgggccgtca cggcataatt gccccagcgg
     2101 tccgccgcgc gaatagtagg gacacgcccg cgatggaaca actcctcaga cgtctttcca
     2161 ttctcggcac catcgggatg tttaccgtcc tgctgatggg cgccaccgta accgacaccg
     2221 gatcacagac cggatgcggc cgatcctggc ccctctgcca cggaaacttc ctgccggcct
     2281 cggtccgcga gtcggtcatc gagttcagcc accgggcagt gaccggcgtg atcggcatcg
     2341 tgatcgtggc tcttgcgatc ctctcgatcc ggatccggca caagtacccg gcgctgctgc
     2401 cgctggtccc gatcatggtc ggggcgctgc tgcttcagtc gggcatgggc gcgtgggcgg
     2461 tcaagtatcc gcagtcggcg gccgtgctcg cgctgcactt cggcttctca ctggtcgcgg
     2521 tcgcgggcgt tttcctggtc atgcgcgtga ttgacgagca atccagcggc aggtatgtcg
     2581 aaaaaccgcc ggcgccaccg ctgctcaagg cgttgttctg ggccagcgtg gtcgtcgtct
     2641 acgttgttgc ctattccggc gcgtacatca agcacgccca tgccgaagat ggctgccgga
     2701 gctggccgct atgcgacggg caagtctggc cgggtctgag cggagacaac ggcgtgatct
     2761 tcatgcaccg gctcgccgca acgggaagcg tgctcctcct gatcgggatt gcctggtacg
     2821 cgcgccggta ccgggagatt cggcccgacc tctacaacgt ctcgctcctt gccgttctgg
     2881 cggtcgcctg ccaggcggtc gtcggcggga tcgtggcggc gacgaacatg cagctgatct
     2941 cgacgctgct tcacgcggga ctgatgactg tgctcttcct ggtcctttgc gaggggtgcc
     3001 ggttgacgat cccgcaacgg gagccgaatc gggcgtggtc gcccgcccaa caggctgttg
     3061 cgccgcaggg cgctgattga ccctcacaag cggttgccat atacttcagg gctgacgccg
     3121 gggaagaccc ggcgtttcag tgtcggatag gcggggcgcg atggatcccc aatttgccgg
     3181 ccaggtggac ctcgataacg actcgatcgt caacgtcgcg gcgttattgc gagagcagac
     3241 gggcgcaact cgctcgtatc ggtttcagat cgaccgtttt cccctggacg atgatctcga
     3301 tgcggttggc gtggcaggga gcctgcggct gacccggttg agtgacgcaa ttttggcctc
     3361 ggtttctggc accgcgagcg tcgtgctcga gtgccagcgg tgcctggagc aattctcgct
     3421 cccggtatcg gtcaaattcg aagagccgtt ccgaatcgcg tacgatgtgc gccgtggaac
     3481 ggcgatcgaa tcggaaaccg aggggatcga cgagcgaccg gaaatcactg aaaaccatga
     3541 gttggatttc agcgagccga tgcgacagga aatcatcgtg gcacttccga tgcgccccgt
     3601 ttgtggcccg gattgccccg gacctccgat cttcgccgat ggcgatgagg aggaaggcag
     3661 cggacagttt tcggcgctcg
//
LOCUS       DATAOZ010000009         6622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7634061, whole genome shotgun sequence.
ACCESSION   DATAOZ010000009 DATAOZ010000000
VERSION     DATAOZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7634061"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VHR64_00180"
     CDS             complement(<1..638)
                     /locus_tag="VHR64_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEX3301270.1"
                     /translation="MKKLTLVRAEEVMEFAQRQVLNLITSYPDFYPMFTEGGKWKHDK
                     PAWTRWCDGFLPGMMWIFARRTRDPEWTNRAVHYSRGLEERKLDRDVHDLGFVFWSTY
                     KRWFDLTGDPALNDVVITAGTTMGYRFNEQGNYLRSFVAPESCFIDIMMNVGIVFYAA
                     QQTKDPDLLRIAIEHCLTTRRFLVRGDGSTSHEGIFDLETGMFLRQTTHQGWR"
     gene            complement(1044..1379)
                     /locus_tag="VHR64_00185"
     CDS             complement(1044..1379)
                     /locus_tag="VHR64_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX3301271.1"
                     /translation="MSGGNTPTIPALIAQHSGELSDLCHRYLVRRLELFGSATGEQFD
                     PIASDLDFLVEFDMTADVDSWLSIYFSFRDALEALFGRSVDLVSFRAVENPHVRSSIE
                     QQRVLVYAA"
     gene            complement(1430..2218)
                     /locus_tag="VHR64_00190"
     CDS             complement(1430..2218)
                     /locus_tag="VHR64_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX3301272.1"
                     /translation="MTDPASNRVLFSIDGRIATLTINRPEKLNALDPGMLEAMAGHLR
                     GLDRSTDARVLIVSATGDRAFCVGADIGVWSSLEPIEMWRDWVRTGSAVFDQLARLRI
                     PTIAALNGYTFGGGLELALACDLRIAADTVQLSAPETKIATVPGWGGTYRLSELIGPA
                     RAKQMIFTGARITAETAATWGLVNEVVAGDHVPSRVREVASEIAANAPVAVQLAKAAI
                     DGARGIGSGVTFEAMASALAAMTEDGQEGVRAFRDKRTPEFNDR"
     gene            complement(2316..3917)
                     /locus_tag="VHR64_00195"
     CDS             complement(2316..3917)
                     /locus_tag="VHR64_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HEX3301273.1"
                     /translation="MEDAAALIPDGATVSVSSSSGLGCPDAMLRAIGERFRDTGSPRN
                     LTTIHPIAAGDMYGIDGIDHIAEPGLIKRVIAGSFPSGPSSMPSPKIWQMIHENQIEA
                     YNIPSGILFHMHLDAAAKRPGVLTQVGIDTYVDPRRDGGRMNERTTEDVVELVQFDGQ
                     EWLYFRAIPIDIAIIRGTTADEFGNISMEHEGAYLGALDQALAARNNGGIVIAQVKRT
                     TARGTIPTQDVRVPGNLVDYVVVDHEQMQTTQTRYDPAISGELRAPLDSFEPVEWGTD
                     KVIARRAAMELLDGDAVNLGFGISALVPRILLEEGHGGDVTWAIEQGAVGGVPLLGFA
                     FGCAANAMGIMPSPMQFVYFQGGGFDRTFLSFLQVGADGSVNVSKLTAKPHVTAGVGG
                     FVDITANARNIVFSGYLTAGGIDLALVDGRLEIRREGKARKFVPKVEHVTFSGRMALE
                     RGANVTYVTERCVLKLRPEGLTVTEIAPGVDLERDVLRQVDIPLQVAEDLRLMDERLF
                     RPEPMGLKLRRAGEKSSRRAAKEIV"
     gene            complement(4040..5044)
                     /locus_tag="VHR64_00200"
     CDS             complement(4040..5044)
                     /locus_tag="VHR64_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3301274.1"
                     /translation="MARWGLVGASNIARQYMIKAINAQPDAEVIGVVSSNQERGAAFA
                     AEQDVPRHYDSLGALLADSDIDCFYISTTNEHHHDQTIAAAKAGKHVLCEKPLALTLA
                     DAQEMVAACRKAGVVMATNHHLRNAGLHRRMRDLIEKGAVGEILAVRVFHAVFLPEFL
                     QGWRIDSAAAGGGVVLDITVHDADTVRFVTGDNPTEVTAMTATHGMGKSGLADTAMGV
                     MRMKQGALVQFHDAFTIKHMPTGFEVHGTEGSLVARNCMTQNPVGELYLRRGTEEREI
                     DDFDRDDLYTRSVRNFMAAVNGTGQPSATAEDGLWSLATALAVAESAGSGRVVPVSIA
                     "
     gene            complement(5120..6124)
                     /locus_tag="VHR64_00205"
     CDS             complement(5120..6124)
                     /locus_tag="VHR64_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301275.1"
                     /translation="MHGVSIALVVVLVLFATIFQTSLAPGTGAGEKSNNGSAVNASGT
                     PGALTPTPTGDGTLAMAGPSPEASPAVVANVGADTEPILPNYRIVSYYGHPNDDTMGI
                     LGQFNKQDLLEQLMDEAAAYERADPSRPVMPAFEIVFSVAQSDPGADGKYLLHTDDAT
                     MRDYIQFTQENNILLIIDIQFGKSTVQDEMAMIEDYLREPNVHLAIDPEFAWGPDQQP
                     GDFGTVDATAINYAQDELARIVAENNLPPKVLVVHRFTDGMVTNIKKVKQVENVQFVL
                     DFDGFGDPVSKQQGYDLYVRDGGVPYGGIKLFYDQDKPLMKPEDVVDLEPAPDFVMYQ
                     "
     gene            complement(6386..>6622)
                     /locus_tag="VHR64_00210"
     CDS             complement(6386..>6622)
                     /locus_tag="VHR64_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301276.1"
                     /translation="ATTVPATSVPTVTATPDSSLGDAASTDDVTDLPKTGAPDVTNSP
                     DRLGRALELLVGSLILTVLIGPVWGARNLTMRGD"
BASE COUNT         1230 a         2137 c         1975 g         1280 t
ORIGIN      
        1 cgccagccct ggtgggtcgt ctgccgcaag aacatgccgg tctccaggtc gaagatccct
       61 tcatgtgagg tgctaccgtc accacgcacc aggaagcgcc gtgtggtcag gcagtgctcg
      121 atcgcgatcc gcagcaggtc agggtctttg gtctgctggg cagcgtagaa gacgatccca
      181 acgttcatca tgatgtcgat gaagcagctc tccggcgcga cgaacgagcg caggtagttg
      241 ccctgctcgt tgaaccgata ccccatggtc gtcccggccg tgatcacgac gtcgttcagc
      301 gctggatcgc cggtcaggtc gaaccagcgc ttgtaggtcg accagaagac aaagccaagg
      361 tcgtgcacat cgcgatcgag cttgcgctcc tcgagtccgc gggagtaatg cacggcacga
      421 ttggtccact cggggtcgcg ggttctgcgg gcaaagatcc acatcattcc cggcaggaag
      481 ccgtcgcacc agcgggtcca ggccggcttg tcgtgcttcc atttcccacc ctcggtgaac
      541 atcgggtaga agtccgggta ggaggtgatc aggttaagta cctgccgctg cgcaaattcc
      601 atgacctctt cggcgcgtac cagagtcaat ttcttcaaga atcggtctcc gattgcccgg
      661 gcccacctgg taaacccgga tgacactcgc cttcactgaa cggcagccag tccgcttagc
      721 tttgggcaag atcacctaaa tcgatgcgca atttgggaat gtcatcgcgg catatcgccc
      781 agatgatcgc gtaatccact cgctcgtagc cgtgaactat tacgttgcgg agatccacaa
      841 atccgcgaag tgacgcaatt ccgctggcca gttccggttc ggcatgttca aactggcgca
      901 gtgcttcccc aacaatttca agataacgct ctaccaggcg gcggctatct ttatcacgga
      961 ggaattggtc gaacgtggta ccgtgaacga ccctgaggac gtcagtacag gcctcagcag
     1021 cattccaaac gtacgacctg gatctaggcc gcatacacca agacccgctg ttgctcgatg
     1081 ctggaccgga cgtgtgggtt ctcgacagcc ctgaacgaca cgagatcgac cgacctgccg
     1141 aatagggctt ccagtgcatc tctgaaagag aaatagattg acaaccacga gtcgacgtcc
     1201 gcagtcatat cgaactcaac gagaaaatcg aggtcgcttg caatagggtc gaattgctcg
     1261 cccgtcgcag atccgaaaag ctccagtctg cgcacaaggt aacgatggca gagatccgac
     1321 aactcgccgc tgtgctgagc aattagtgcg ggtatggtcg gtgtatttcc tccagacatc
     1381 tgcctatccg ggtttcacaa ggaccgtctc atccattcta cccgcacaat taccggtcgt
     1441 tgaactccgg ggtgcgctta tcgcgaaatg cccggacgcc ttcctggccg tcctcggtca
     1501 tggcggcgag cgcgctggcc attgcctcga atgtcacgcc tgagccgatg ccgcgcgcgc
     1561 cgtcgattgc cgccttggcg agctggactg cgaccggcgc gttcgccgcg atctcgctcg
     1621 caacctcccg aactcgcgac ggcacatgat cgcccgcaac gacctcgttg acgagacccc
     1681 acgtggccgc ggtctcagcg gtgattcgcg cgccggtgaa gatcatctgc ttggctcgcg
     1741 cggggccgat cagctccgac aagcgatagg ttccgcccca gcccggcaca gtcgcaatct
     1801 tcgtctccgg cgcggagagt tgcaccgtat ccgccgcgat cctgagatcg caggccagtg
     1861 ctagttcgag gccgccgccg aaggtgtagc cgttgagcgc ggcgatcgtg ggaatacgca
     1921 ggcgagccaa ttgatcgaag acggccgagc cggtccgcac ccaatcccgc cacatctcga
     1981 tcggctccag gctcgaccag acgccgatat ccgcgccgac gcagaacgcg cgatccccgg
     2041 tcgcagacac gatcagcacg cgcgcgtcgg ttgaccggtc gagcccgcgc aaatgcccgg
     2101 ccatcgcctc gagcatgccg ggatcgagcg cgttcagctt ttcgggacgg ttgattgtca
     2161 gagttgcaat ccgaccgtcg atcgaaaaga gcactcgatt cgacgccgga tcggtcactt
     2221 cctgatccgc agcgtccgca atatcttgtc gagccactcg ttaagcgtgt ctgtgatcga
     2281 tttcttatcg ctggaaccag ggttcttact cgacatcaca cgatctcctt tgctgctcgc
     2341 ctgctcgact tttcgcctgc tcgcctcaac ttcagcccca tcggctcggg ccggaacaac
     2401 cgctcgtcca tcaatctcaa atcctcagcc acctgcaacg ggatatcgac ctgcctgagg
     2461 acgtcccgct ccagatcgac gccgggtgcg atctcggtta cggtcagacc ctcgggccgc
     2521 agcttcagca cacaccgctc ggtcacatag gtgacgtttg cgcctcgttc gagcgccatc
     2581 cggccgctga acgtgacgtg ctcgacctta ggcacgaact tgcgcgcctt gccctcgcgg
     2641 cggatctcga gtcgaccatc gacgagcgcc agatcgattc cgcccgcggt caggtacccg
     2701 ctgaagacga tgttccgcgc gttcgccgtg atgtcaacga agccgccgac gcctgccgtc
     2761 acgtgcggtt tggcggtcaa cttcgagaca ttgactgagc catccgcgcc gacctgcagg
     2821 aacgacagga aggttcggtc gaacccgccg ccctggaaat agacgaactg catcggcgac
     2881 ggcatgatcc ccatcgcgtt cgccgcgcag ccaaaggcga agcccagcaa cggcacgcct
     2941 ccgaccgcgc cctgctcaat cgcccatgtc acgtcaccgc catgcccctc ttcgagcagg
     3001 atccggggta cgagcgccga aatgccaaag ccgagattga ccgcgtcgcc atccagcaat
     3061 tccatggcgg cgcgccgggc aatgacctta tcggttcccc actcaaccgg ctcgaacgaa
     3121 tcgagcggcg cccgcagctc cccgctgatg gccgggtcgt aacgcgtctg ggtggtctgc
     3181 atctgctcgt gatcgactac gacgtaatcg accaagttgc ccggcacgcg cacgtcctgc
     3241 gtcgggatcg tgccccgggc tgtcgtccgc ttgacctgcg cgatcacgat gccgccgtta
     3301 ttgcgagcag ccagtgcctg atcgagcgcg ccgagatatg cgccctcatg ctccatcgag
     3361 atgttaccga actcgtcggc agtggtcccg cggataatcg cgatatcgat cgggattgcc
     3421 cggaagtaga gccattcctg gccgtcgaac tggaccaact caacgacgtc ctcggttgtg
     3481 cgctcgttca tgcgcccacc gtcccgccgc ggatcgacgt aggtgtcgat tccaacctgg
     3541 gtcaggacgc ccggtcgttt ggctgccgcg tcgaggtgca tgtggaagag gatgccgctc
     3601 ggaatgttgt acgcctcgat ctggttctcg tggatcatct gccagatctt cggcgacggc
     3661 atcgatgacg gcccggaagg gaacgagccg gcaatcacgc gcttgatcag gccgggctcg
     3721 gcaatgtgat cgatgccgtc tatgccgtac atatcgccgg cggcgatcgg gtggatcgtg
     3781 gtcaggttgc gtggcgagcc ggtgtcgcgg aatcgctcac cgatcgcgcg cagcatcgcg
     3841 tcggggcagc cgaggccgct ggaggagctg accgagacgg tcgccccatc cgggatcagc
     3901 gcggcagcgt cttccatcga cacgattttc gaccgcccgg ttgccacgac ctaccctgcg
     3961 cttccgtatg ccagcactcg tttgagcaag tatacgaagg cgcgtgattc accagaagga
     4021 gcggtccgag cgtgcgcttt caggcgatgg atacaggaac gactcggccg gaaccggctg
     4081 actcagcgac tgcaagcgcc gttgccagcg accagagtcc gtcctcggcg gttgccgagg
     4141 gctgccctgt cccatttacg gcagccatga aattgcggac cgagcgggtg tagaggtcgt
     4201 cgcggtcgaa atcgtcgatc tcccgctcct cggtgcctcg ccgcaggtac agctccccga
     4261 ccgggttttg ggtcatgcaa ttccgcgcga caagcgagcc ctcggtgcca tggacctcaa
     4321 acccggtcgg catgtgcttg atcgtgaagg cgtcgtggaa ctggaccagc gcgccctgct
     4381 tcatacgcat cacacccatg gccgtatcgg cgaggccgct cttgcccatc ccgtgcgtcg
     4441 cggtcatcgc cgtgacttca gtcgggttat cgccggttac aaagcgaacg gtgtcggcat
     4501 cgtgcactgt tatgtccagc acaactccac cgccggcggc tgcgctgtcg atccgccacc
     4561 cttgcaggaa ctcaggcaaa aagaccgcgt ggaagacgcg aaccgccagg atctcgccga
     4621 ccgcgccctt ctcgatgagg tcgcgcatcc gtcggtgcag ccccgcattg cgcaggtgat
     4681 ggttcgtcgc catcaccacg ccagccttcc ggcacgcggc gaccatctcc tgcgcgtctg
     4741 ccagtgtcag cgccagcggt ttctcgcaga gcacatgttt gccggccttc gcggccgcga
     4801 tcgtctggtc gtgatgatgc tcgttggtgg tgctgatgta gaagcagtcg atgtcggaat
     4861 cggccagcaa cgcaccgaga gagtcataat gccgcggcac atcctgctcg gcagcgaacg
     4921 cagcgccgcg ctcctgattg gagctgacaa cgccgatcac ctcagcgtcg ggctgcgcgt
     4981 tgatcgcctt gatcatgtac tgccgggcga tattgctcgc cccgaccaga ccccatcgtg
     5041 ccatgacgta cctcgcttcg attttcggtt ttcgggaacg agcgtaactg ctctcctgaa
     5101 cgcctcccga ctgctcacct cattgataca tgacaaaatc gggcgctggc tccaggtcga
     5161 cgacgtcttc cggcttcatc agcggcttgt cctgatcata gaagagcttg attccgccgt
     5221 acggaacgcc gccatcccgg acatagagat cgtatccctg ctgtttgctg actgggtcac
     5281 cgaagccgtc aaaatcgagc acgaactgga cattttcgac ctgcttgacc ttcttgatgt
     5341 tggtcaccat gccatcggtg aaccggtgca cgaccagaac cttgggcggc aggttgttct
     5401 ccgcgacgat ccgggccaac tcgtcctggg cgtagttgat ggcggtcgcg tcgaccgtgc
     5461 cgaagtctcc cggctgctga tccggtcccc acgcgaactc aggatcgatc gcgaggtgga
     5521 cattcggctc gcgcaggtag tcctcgatca ttgccatctc atcctgcacg gtcgatttgc
     5581 cgaactggat gtcgatgatc agcaggatgt tgttctcctg cgtgaactgg atgtaatccc
     5641 gcatcgtggc gtcgtcggtg tgcagaaggt acttgccgtc cgcaccgggg tccgactgcg
     5701 ccaccgagaa gacaatctcg aacgccggca tgaccggtcg acttggatcc gcccgctcgt
     5761 aggccgcggc ctcgtccatc aactgctcga gcagatcctg cttgttgaat tggcccagga
     5821 tccccatcgt gtcgtcgttg ggatggccgt aataggagac gatgcggtaa ttgggcagga
     5881 tcggctctgt gtccgcgccg acgtttgcaa ccaccgcggg cgatgcctcg ggcgacgggc
     5941 cggccatcgc caacgtgcca tcgccggtcg gcgtcggcgt gagcgcgccc ggggtcccgg
     6001 atgcattcac agctgaaccg ttattcgact tctcgccggc tccggttccc ggggcgagac
     6061 ttgtctggaa gatcgtcgcg aagagcacaa gcacgaccac gagcgcgatc gagacaccat
     6121 gcatcgaagg gacccacgcc ggcggcggtc cgacttgcgg ccgctgtgga agcgctcgtt
     6181 gctcgggcgg catattgagc cactcgcggg aaaacggttt tggaccagca gattgccggt
     6241 cgatttgggc tgaagctcga cgtatcaccg gtcgtcgcac agggtggtcc gcgggtaacc
     6301 gattgtcacg ccgtcccgca aatccgcgcc agaaaccagc gggattcgac tcaggccggc
     6361 gtgacagacg atgtcgaacc ttcctctagt cgccccgcat tgtcaagtta cgcgcacccc
     6421 agaccggacc gatgagcacg gtgagaatca gggatccgac caggagctca agggcacggc
     6481 ccagtcgatc gggcgaattc gtcacgtccg gcgcgccagt cttcgggaga tcggtgacat
     6541 cgtcagtgct ggcagcatcg ccgagcgacg aatccggcgt ggccgtgacc gttggcacgc
     6601 ttgtggccgg caccgttgtc gc
//
LOCUS       DATAOZ010000010         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4478428, whole genome shotgun sequence.
ACCESSION   DATAOZ010000010 DATAOZ010000000
VERSION     DATAOZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4478428"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /locus_tag="VHR64_00215"
     CDS             <1..1529
                     /locus_tag="VHR64_00215"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3301277.1"
                     /translation="LTMDGIVKMFPGSVALDGAYLHVQRSEVHAVVGQNGAGKSTLMK
                     ILNGAYRRDSGQVVLDGQDIDFVSPLQAQRNGISTIYQEINLVPFRSVAENIFMGREP
                     RRLGLINWRAMNQRAEVLLARLNVHIDVRQPLMAYNVALQQMVAIARAISFESKIVIM
                     DEPTSSLDDREVATLFDVIRNLKASGVSVIYISHRLDELHQVCDQITIMRDGKTIDER
                     PLASITKLEIVAQMLGKELGEVRREGATGFQDREAEMDVAPLLEARRVNRPRMLRDAS
                     VTVRPGEIVGLAGLLGSGRTELARVIFGADRMESGEIRYAGRDRAFKSPSDAIRAGIG
                     LCPEDRKVDGIVPYLSVRENLTLAALPVLARNGIVDPDKQREIVDRFIARLDIKTSNP
                     DQPIRELSGGNQQKVLLARWLCLNPKLLILDEPTRGIDVGAKAEIQGLIDELADAGLG
                     VLMISSEIEEITEGSDRVTILSEGVTVAELDRNQISQDRIIHAMARGGETDEPGDMSA
                     "
     gene            1526..2536
                     /locus_tag="VHR64_00220"
     CDS             1526..2536
                     /locus_tag="VHR64_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301278.1"
                     /translation="MMSTGATSAVPREHGRVHLPSILQFAGPTSGALIALILLSIYNA
                     IFTTNFLTLATLRVNLTQVSTVVLVAVGMTLVIATAGIDLSVGALMAISGALAPLIFM
                     SDRPFLASDWVAVPLAIIIPLLVAGAFGLFNGLLVTRFEIQPIVATLVLFLGGRGIAK
                     ALLSGQLREFRNANFDYIGTGRPLGIPVQVILMLVVVLVAAWVMRSTAFGRYVLATGG
                     NEEAARLAGVPVNRTRMIVYGISGLLAGLAGLIVISLNSSTDPNLVGLNMELDAIAAA
                     AVGGTVLTGGRATIFGTFVGALLVHQIRYTLLSHGVAEGTTRIVTAAVILGAVLLQRR
                     TD"
     gene            2550..>3446
                     /locus_tag="VHR64_00225"
     CDS             2550..>3446
                     /locus_tag="VHR64_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301279.1"
                     /translation="MAAQAPVAPNPRSIRLVFYLQQQGALIALVIIVAFGLLRYGENF
                     RGSFNIWEMLRNNSYFGLIALGMSFVIMSGGIDLSVGAVAALSAVCAARLSEHGIWVG
                     TLGAVAMGMAIGIINGGLIARFKVQPFIVTLAMLLACRGFALNLANNANVSVDFASGY
                     IDLGQRNVGRVPLPFLIMVIAYIIGSIVLNFTRFGRHVLAIGGNEEAARLAGLPVART
                     IVVVYAVSGACAGLAGAILAALAYTGSPNFAIGWELSAIAAVVVGGTLLTGGQGSVWT
                     SLVGVLLLGLIFNELNFERAKGT"
BASE COUNT          588 a         1067 c         1109 g          682 t
ORIGIN      
        1 tgttgaccat ggacgggatc gtcaagatgt tcccgggctc cgtcgcactc gacggggcct
       61 atctgcacgt gcagcgcagc gaggtccacg cggtcgtcgg ccagaatggg gctggcaagt
      121 ccaccctgat gaagattctc aacggcgcct accgcaggga cagcggtcag gtcgtcctcg
      181 atgggcagga catcgatttc gtctctccgc tgcaggcgca gcgtaatggc atcagcacca
      241 tctaccagga gatcaacctt gtcccgttcc gctcggtcgc tgagaacatc ttcatggggc
      301 gggagccgcg ccggctcgga ctgatcaact ggcgggcaat gaatcagcgg gccgaagtac
      361 tgctagcgcg gctcaacgtc cacatcgatg ttcgccagcc gctgatggcc tataacgtgg
      421 cgctccagca gatggtcgca attgcccgcg ccatctcgtt cgagagcaag atcgtgatca
      481 tggacgagcc aacctcgtcc ctcgacgatc gagaggttgc gacccttttc gacgtcattc
      541 gtaatctcaa agcgtccggc gtgagcgtga tctatatctc gcaccggctg gacgagcttc
      601 accaggtctg cgaccaaatc acgatcatgc gggacgggaa gaccatcgac gagcgaccgc
      661 tggccagcat cacaaagctg gagatcgttg cccagatgct cggcaaggag ctcggcgagg
      721 tacgcaggga gggcgcgact ggattccagg accgcgaggc cgagatggac gtcgctccgc
      781 tccttgaggc ccgccgcgtc aaccggccgc gcatgctccg cgatgcctcg gtgaccgtgc
      841 gtccgggcga gatcgttggc ctggccgggc tgctcggctc tggtcggact gagttggctc
      901 gcgtcatctt tggcgctgat cgcatggaat ctggcgaaat ccgctatgcc ggacgagatc
      961 gcgcgttcaa aagcccgtcc gatgcgatcc gggcagggat cggcctctgc ccggaggatc
     1021 ggaaagtgga cggcatcgtg ccatatctct cggtccggga gaacctcacc ctggccgccc
     1081 tgccggtgct ggcccgaaat ggcatcgtgg atcctgacaa gcagcgcgag atcgtcgacc
     1141 gattcattgc ccggctagat atcaagacgt cgaatccgga tcagccgatt cgcgagttgt
     1201 cgggcgggaa ccagcaaaag gtgctgctcg cgcgctggct ctgcctgaac ccgaagctat
     1261 tgatcctgga cgagcccacc cgcggtattg atgtgggcgc caaggccgag atccaggggc
     1321 tgatcgacga attggccgac gccgggttgg gcgtgctcat gatctcctcg gagatcgagg
     1381 agatcaccga gggcagcgac cgcgtcacca tcttgagcga aggcgtcacg gtagccgaac
     1441 tggaccggaa tcagatttca caggatcgga ttatccacgc aatggcacgg ggcggcgaaa
     1501 ccgacgaacc gggggacatg agcgcatgat gagcaccggc gcgacatccg ctgttccccg
     1561 cgagcacggt cgcgttcacc tcccttcgat cctgcagttc gccgggccga cgtccggcgc
     1621 gttgattgca ctgatcctgc tctcaatcta caacgccatt ttcacgacca attttctgac
     1681 gttggctacg ctccgggtca acctgacgca ggtctcgacc gtcgtcctgg tcgccgtggg
     1741 aatgacgctg gtgatcgcca ccgccgggat cgatctctcg gtcggcgcac tgatggcgat
     1801 cagcggcgcg cttgctccgc tgatcttcat gagcgatcgg ccattcctag cctccgactg
     1861 ggtcgcggtg ccgctggcaa tcatcatccc gctcctggtc gccggtgcat tcggactgtt
     1921 caacggtcta ttggtaaccc ggttcgagat tcagccgatc gtggcgacgc tggttctctt
     1981 cctgggtggt cggggcattg ccaaggcact cctctcgggt cagctgcgcg agtttcgtaa
     2041 cgcgaacttc gactacatcg gcaccggccg accactcggg ataccggtgc aagtcatcct
     2101 gatgctggtc gtggtcctcg ttgcggcatg ggtgatgcga tcgactgcct tcggccgata
     2161 tgtgctggcg actggcggca atgaggaggc cgcacggttg gcgggtgtcc cggtcaaccg
     2221 gacgcggatg atcgtgtacg gcatcagtgg cttgctcgcc ggattagccg ggctgattgt
     2281 gatctctctc aactcctcaa ccgatccaaa cctggtcggg ctcaacatgg agctggacgc
     2341 gatcgccgcg gcggcagtcg gtggcacggt tctgaccggc ggacgggcga cgatcttcgg
     2401 cacgttcgtc ggcgccctgc tcgtccacca gatccgttac accttgctct cccacggcgt
     2461 tgcggagggc acgacacgga tcgtaacggc ggcggtgatc ctcggcgcgg tgcttctgca
     2521 gcgtcggacc gactgaggag gacactcgca tggctgccca agcgccagtc gccccgaacc
     2581 cgcgaagcat ccggctcgtc ttttatctgc agcagcaggg cgccctgatc gctctcgtga
     2641 tcatcgtggc gtttggcctg ctgcgctacg gagagaactt ccgcggctcg ttcaacattt
     2701 gggagatgct ccgcaacaac tcgtacttcg gactgatcgc gcttggcatg agcttcgtga
     2761 tcatgtcggg tggcatcgat ctgtcggtgg gggcggtcgc ggcgctctca gccgtctgcg
     2821 cggcacgatt gagcgagcac ggaatctggg tcggcacact tggcgcggtc gcgatgggca
     2881 tggcgatcgg catcatcaac ggcggcctca ttgcccgttt caaggtgcag ccgtttatcg
     2941 tcacgctagc gatgctgctg gcctgccggg gatttgcgct caacctggcc aacaacgcga
     3001 atgtctcagt cgacttcgcg agcgggtaca tcgacctcgg ccagcgcaat gttggtcggg
     3061 tgccactgcc cttcctgatc atggtcatcg cctacatcat cggctcgatc gtgctcaact
     3121 tcacccgctt cgggcgccat gtcctggcaa tcggcgggaa tgaggaggcg gcgcgcctgg
     3181 ccggactccc ggtcgcgcga acgatcgtcg tggtttacgc agtcagcggc gcgtgcgctg
     3241 gtctggccgg ggcgattctg gcggcactag cctacaccgg gtcgccgaac ttcgcgatcg
     3301 gctgggagct ctcagcaatt gccgccgtgg tcgtcggcgg cacgctcctg accggtggtc
     3361 agggatcagt ctggacgtcg ctggtcggcg tcttgctgct gggattgatc ttcaacgagc
     3421 tgaacttcga gcgcgccaag gggacg
//
LOCUS       DATAOZ010000011         5316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7635000, whole genome shotgun sequence.
ACCESSION   DATAOZ010000011 DATAOZ010000000
VERSION     DATAOZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7635000"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VHR64_00230"
     CDS             <1..506
                     /locus_tag="VHR64_00230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3301280.1"
                     /translation="AFRELIPVIPESVQLVIGGAPHSANPIVFPRLEPVIRELGLESR
                     VILTGRMSEEDKVALMQGAAVYVTPSLYEGFGLSVLEAMACGVPVIAANRTSLPEVVG
                     DAGLLVEPRIEPLAAAMRAILTEPDLATALSSRGLERARQFYWRLTAEQTVAVYEHVL
                     SERGRNH"
     gene            508..1083
                     /locus_tag="VHR64_00235"
     CDS             508..1083
                     /locus_tag="VHR64_00235"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX3301281.1"
                     /translation="MGSIVERPLRDALRMIVPGPVVLVSSMSKGQPNVMTAAWLAAQS
                     LSPTLISVAIHPDRLTHQFVSAAEEFVVNVPVMDSLSIVHQAGLISGRDGDKFAALGL
                     EPDESVIVEPPRVAGSAGYIECRVQDRVSAGDHDLFIAEVVAVAADDESFDGHWNIAV
                     DAGRLLHHLGADRYAGLANQYRVAPSEAEDA"
     gene            complement(1295..2509)
                     /locus_tag="VHR64_00240"
     CDS             complement(1295..2509)
                     /locus_tag="VHR64_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301282.1"
                     /translation="MKFRKPTDNSNSMIMLGGWLFADLLLGLAMLFLTSGTSGSPPPT
                     PTPTSTPNQLATAQVRAARTATVQAENVANLNATVESAADMAQATEAARATEDARLSN
                     MLTATARADATKQAMSSDQQATAAAEATAEAISSEATMAAFATQQAQSNADVDQVTRD
                     LATAAAQATADAQSARATIAAQETQAAEVAAIATQNAESGANALATANAAATEIVEIA
                     AIATQNAESGANDLATAQARAEAAQATAEALTQSQAVSDSDKATAQAEVANIQATVES
                     VRATATVISQRAGLNSIDQGYAEVTIKVDASGLLSGDKKAEDDATKAIQSALKDYDGC
                     RVGITLTFGWDPDINRGLAIADAINKLLPDAEPKMFEEAAYENFGNLQPSGTVEIRMY
                     FFRGCQALDNSP"
     gene            complement(2539..4260)
                     /locus_tag="VHR64_00245"
     CDS             complement(2539..4260)
                     /locus_tag="VHR64_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301283.1"
                     /translation="MVQFRARKSNEPFPRIKGDNHSAFDTVPSSDVSTIPLPRSGPRP
                     ATTRDLADEINGLAASLEEIDSRSTERLRDLASSLSTEEGRLRWADVDLRRAFNSEYL
                     AHFYAVRREGGYAPTSVAVADRIRNVLVLLPIALTWFALYESTKSYREYIDAHPDQIS
                     QPFLLLWQRGFDGRGGILAASFSTVAIIDAMIIGVIIALTFYSHGRREHREESIDRTA
                     QKFQADVDNILAEATVALAPDRAGRPAMLARSVERLAERFEHNSQELIGRLRAEHDRL
                     ESISNRREKEFADFGVFASGMRAGAEETHRLLIELRQVSTALQQALEDMTSEVGVTGD
                     QNKSLLSAVTALERLVATGIQSDHGVTRRLAEAAEVLSEAAERSLAGSEAAAQAGRVA
                     TEAVRGIAEIGASLASGQNRVEHAIANESEANTRLAEALRSGMTGVSTTARTLAEIQA
                     SLVGIRDEFREIAQSNQDYATSLSRMLTEQTSISTGIAKMARDIGSAGASSQQQQQNM
                     NQELSSLIKRLDNIAPTPQAGPGGFESVQTSDGGVRRVLTNAPDFLSDPVESPGGRDS
                     GNRGRRD"
     gene            4412..>5316
                     /locus_tag="VHR64_00250"
     CDS             4412..>5316
                     /locus_tag="VHR64_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301284.1"
                     /translation="MTLQTLYDAAMLRGPRSVPLLGRLGGGTIATISDSQPFRVVGSN
                     AVVYQLRQATGKVVALRCWLKDEIPPDLVERYRALGQPSTLRRLHGAERSPVIGSISL
                     HADGVVIEADDLRSSTRPIVVLDWLMGPTIMAAVDRACRSQDTGYLAALAQAWRGAVS
                     AAGRVGFVHGDLTADNAIVRPKEGIAYVDYDSAYWPDAPSVPHLDPAASYRHPRGITS
                     RPEHADEFAALLVYVSLRILAVWPELRADHGQAATMRGAGLLFQPRDLAHPDGSQLFG
                     KLRVVNDSQVQGLVGILREACLAHP"
BASE COUNT          825 a         1672 c         1778 g         1041 t
ORIGIN      
        1 gagcatttcg ggagctcatt ccagttatac cggagtcagt tcaactcgtg atcggtggag
       61 cgccgcacag cgccaatccg atcgtcttcc cacggctgga accggtaatc cgggaactcg
      121 ggctcgaatc ccgcgtgatt ctgaccgggc ggatgtcgga ggaggacaag gtcgcgctca
      181 tgcagggtgc ggccgtctac gtgacgccgt cgctctatga gggttttggg ttgagcgtgc
      241 tggaggcgat ggcttgcggt gtcccggtca tcgcggccaa tcggacgagc ttgccggagg
      301 tcgtcggcga tgccggattg ctggtcgaac cgcggattga gcccctggca gcagccatgc
      361 gagccatctt gacggagccc gacctggcaa cggcgctcag ctctcgcggg ttggaacgtg
      421 cccggcagtt ctattggcgc ctgaccgccg agcagacagt cgcggtctac gagcacgtgt
      481 taagcgagcg cgggaggaat cattgatatg gggtctatcg tcgagcggcc gctgcgggac
      541 gccttgcgca tgatcgtgcc cggaccggtg gtcctcgtct cgtcgatgag caagggtcag
      601 ccgaacgtga tgaccgccgc ctggcttgcg gcacaaagcc tttcgccgac cctgatcagc
      661 gttgccattc accccgatcg attgacacac cagttcgtct cggcggctga agagttcgtc
      721 gttaatgtcc cggtcatgga ctccctgagc atcgtccacc aggcgggcct gatcagcggg
      781 cgcgatggcg acaaattcgc cgcactcgga ttggagccgg atgaatccgt catcgtagag
      841 ccgccgcgtg tggccgggtc ggctggatat atcgagtgcc gggtgcagga tcgagtgagt
      901 gccggagacc acgacctctt catcgccgag gtggttgcgg tggcggccga tgacgagtcg
      961 ttcgacggcc attggaacat cgcagtcgat gccggccgct tgctgcatca cctgggcgcc
     1021 gaccggtatg ccggccttgc caaccagtac cgcgtggcac ccagcgaagc ggaggacgcc
     1081 taaagcggag tccagaaggg ttgtccgatc gttggcgtag cgcgataccc caccccgccc
     1141 ggactcgatg ccagcaaatt cgtacagtga accaactctg ataccgacct ggctcccgca
     1201 tccgacagtg gggatgcacc aagcggggcg tggtaacccc gcgctaggtt cagggaacgc
     1261 tcatggtaca tgcacgcggc tgtaggcgct cggtctacgg gctgttgtcg agcgcctggc
     1321 agccacggaa gaagtacatc cggatttcga ccgttcctga tggctggaga ttgccgaagt
     1381 tctcatatgc cgcctcttca aacatctttg gctcggcgtc aggcaacagc ttgttgatcg
     1441 cgtcggctat ggcaaggccg cgattgatgt caggatccca gccgaaggtc agcgtaatac
     1501 cgacccggca gccgtcgtag tccttgagcg cgctctgaat cgccttggtc gcgtcatcct
     1561 cggctttctt gtcgccggag aggagaccgc tggcatcgac cttgatcgtg acctcggcat
     1621 agccctggtc gatcgaattc aagccggcac gctgcgagat gaccgttgcc gtggccctga
     1681 ccgactcgac cgtcgcttga atgttggcga cctcggcctg agccgtggcc ttgtcggaat
     1741 cgctgacagc ctgggactgg gtcaacgctt cggccgtggc ttgcgccgct tcggctctgg
     1801 cttgggcggt cgcgaggtcg ttcgccccgg actcagcatt ctgggtcgcg atggcggcaa
     1861 tctcgacgat ctcggttgcg gccgcattgg ccgtcgccag cgcgttcgcg ccggattctg
     1921 cgttctgtgt ggctatggcc gcgacttcgg ccgcctgcgt ttcctgcgcg gcaatggtgg
     1981 cccgggcaga ttgcgcgtcc gcggtcgcct gcgcggcagc ggtggcgagg tcgcgtgtca
     2041 cctggtcgac gtcggcgttg ctctgggctt gctgcgtcgc gaaggcagcc atggttgcct
     2101 cggacgagat cgcctcggcg gtggcctctg ccgcggccgt ggcctgctgg tcgctcgaca
     2161 tcgcctgctt cgtcgcgtcg gccctggccg tagcagtcag catgttcgag aggcgggcat
     2221 cttcggtggc gcgggctgct tccgtggcct gcgccatatc ggccgcgctc tcgacggttg
     2281 cattcaggtt cgcgacgttc tcagcctgaa ccgttgccgt cctcgcggcg cgcacttggg
     2341 cggtggcaag ctggttcggc gttgaggtcg gcgtcggcgt cggcggtggc gagcccgacg
     2401 tgccggacgt caggaagagc atcgccaagc cgagcagcag gtcggcgaag agccagccgc
     2461 cgagcatgat catgctgttg ctgttatcgg ttggtttccg gaatttcacc cgcggaactc
     2521 caatttccgg tcgaccggtt agtcgcgccg gccgcgattg ccggagtcac gtccaccggg
     2581 gctctcgacg ggatcactca aaaagtcggg cgcattggtc aggacgcgac ggacgccacc
     2641 gtccgacgtc tggaccgatt cgaatccacc cggacccgcc tggggcgtgg gcgcgatgtt
     2701 gtcgagtcgc ttgatcaggc tcgacaactc ctggttcatg ttctgttgct gttgctgcga
     2761 cgatgcgccg gcggagccga tatcgcgcgc catcttggcg atgccggtgg aaatcgacgt
     2821 ctgctcggtc agcatccggg agagcgaggt cgcgtagtcc tgattcgact gcgcaatctc
     2881 acggaattcg tcacggatcc cgaccaggct cgcctggatt tccgcaagcg tgcgggcggt
     2941 cgtcgaaacg ccggtcatcc cggagcgcag cgcctcggcc aagcgggtgt ttgcttcgct
     3001 ctcgttcgcg atggcgtgct cgacccgatt ctgaccgctt gccaggctcg cgccgatctc
     3061 ggcgatgccg cggacggcct cagttgcgac acggccggct tgtgcggccg cttcggagcc
     3121 ggcgagagat cgttctgccg cctcggacag cacctctgcc gcctccgcca gccgccgcgt
     3181 gacgccgtgg tcgctctgga taccggtcgc gaccagccgc tccagggccg taacggccga
     3241 aagcagcgac ttgttctgat cgcctgtgac gccgacctcg ctggtcatgt cctccagcgc
     3301 ttgctgcaga gctgtcgaga cctggcgcag ctcgatcagg aggcgatgcg tctcctctgc
     3361 tcccgcccgc atgccgctgg cgaaaacacc gaagtcggcg aactccttct cccgccggtt
     3421 tgagatcgac tccaggcgat cgtgctccgc gcgcaggcgg ccgatcaatt cctggctgtt
     3481 gtgctcgaac cgttcggcca ggcgctcgac actgcgggcg agcatggccg gccgcccggc
     3541 ccggtccggc gcgagtgcga cggtcgcttc ggcgaggatg ttgtcgacgt cggcctggaa
     3601 cttctgggct gtgcgatcga tcgattcctc tcggtgctca cggcggccgt gtgaatagaa
     3661 ggtcaaggcg atgatgacgc cgataatcat tgcgtcgatg atggcgaccg tcgagaacga
     3721 tgccgccagg attccgcccc tgccatcgaa gccccgctgc cagagcagca ggaacggttg
     3781 gctgatctgg tccggatgcg cgtcgatgta ctcgcgatag gacttggtcg actcgtagag
     3841 tgcgaaccag gtcagcgcga tcggcaggag cacgagcacg ttccggatgc gatcggcgac
     3901 ggcgacgctc gtcggcgcat aaccaccctc gcgtcggacc gcatagaagt gggcgaggta
     3961 ttcactattg aaggcccggc gcaggtcgac atccgcccag cggagccgac cctcctcggt
     4021 gctcagggac gaggcaagat cgcgcagacg ctcggtgctg cgcgagtcga tctcctcgag
     4081 cgatgcggcc aggccattga tctcatcggc caggtctcgc gtcgtcgccg ggcgcggccc
     4141 gctgcgtggc agggggatcg tgctcacgtc gctgctcggc accgtgtcga atgcggagtg
     4201 attgtcgccc tttatgcggg ggaagggctc attgctcttg cgagcgcgaa actggaccat
     4261 acgtctcctg gtcgatcggc gttcgctgtc ccctgaaaac acggaccttt gacgcgtgtc
     4321 ggccgatctg aaactcactg taccattgca aaagggcgtc tcgaaacccg tatatgccca
     4381 tcgctgaaca gaatcgggac tatctgcacc catgactcta cagacgcttt acgacgccgc
     4441 aatgttacgc ggtccccgat cagtaccgct tctcgggcgg ctgggcggcg gcacgatcgc
     4501 gacgatctcg gacagccagc ccttccgggt agtcggctcc aatgcggtcg tgtatcagct
     4561 tcggcaagcg acgggcaagg tcgtggccct gcgctgctgg ctgaaggatg agattccgcc
     4621 ggatctggtc gagcgttacc gggcactcgg tcagccgtcc acgctccgtc gcctgcatgg
     4681 ggccgagcgg tcgccggtga tcgggtcgat cagcctgcat gccgatggtg tcgtgatcga
     4741 ggctgatgac cttcgctcgt ccactcggcc gatcgtcgtg ctcgactggc tgatgggccc
     4801 gacgatcatg gccgcggtcg accgcgcctg tcgttcccag gacaccgggt atctggctgc
     4861 actggcgcag gcttggcgtg gtgcagtttc ggctgccggc cgggtcggat tcgtgcatgg
     4921 tgatctgacg gcggacaatg cgatcgtgcg gccgaaggaa ggcatcgcct atgtcgatta
     4981 cgactccgcc tactggcccg acgcaccatc ggtcccgcac ctcgatccgg ccgcctcata
     5041 tcggcacccg cgcggtataa ccagccggcc ggaacacgcg gacgagtttg cggcactcct
     5101 ggtctacgtt tcactgcgga tcctggcagt ttggcccgag ttgagggccg atcacggtca
     5161 ggcggcgacg atgcgcgggg ccggtctgct ctttcagccg cgcgatctcg cccatccgga
     5221 tgggtcgcaa ctgttcggca aactgcgggt ggtcaacgat tcgcaggtcc agggtctggt
     5281 cggcatcttg cgggaggcct gcttggcgca cccgga
//
LOCUS       DATAOZ010000012         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1322196, whole genome shotgun sequence.
ACCESSION   DATAOZ010000012 DATAOZ010000000
VERSION     DATAOZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322196"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2373)
                     /locus_tag="VHR64_00255"
     CDS             complement(<1..2373)
                     /locus_tag="VHR64_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3301285.1"
                     /translation="MATRTDVKMVTGKINGQDVTVLAGTTILEAAREAGIEVPNLCYQ
                     PLLRPWGSCRICTVEILGKRGGLIESCATPLGEGMEVLTHSEPCIDARQFILQMYLID
                     HALDCPTCDKSGECYLQDNTYLHNVNTNPYRRPKFAQPYVHFSDIIDYKWDRCIMCNR
                     CTRVCDEMIGVTAIESSYRGMDAAITPAYGQSLDDTACTRCGMCIAVCPVGALTDRRF
                     AHHPWELDATETICGFCDVGCTISVESNKGIVRRVSHLWERGVNHGYTCEAGRWGHEL
                     LQNPDRIFYPYVRDEDGTVYEVTWDDAINSAAETLAHYQGDQFAAIASADSTNEEVYA
                     LQLFTRAVMGTNNIDRMLTPAQRSVELAVRAGLGDDVSNTNNMQEVFTDVKAAMVVGP
                     DIGKSAPVASYWLYHSHQFREAKTIVISMDDYPLCRRSEFWLKPKPGTAATLLNGIAR
                     AIIDKGLAKGGSEVAGYQQWLTTIQNVSIAQVSADTGVPAADIEAAAVLWASGGAGIS
                     AGADSFPPALIYQTIAHQGSVSENDYYGDPAEISAACNNLAFLTGNIGRAGGGVAAMR
                     GPANYQGATQMGAHPSYLPGGGRVDSAVDRETFGTAWLDRWAEKAKTSNGFLPVRSLP
                     ESRGLSRDELIKAINAGGVKAMIIDGTVAGRLQFIDPELGAALDKLEFLLVFDTFNSP
                     LAQKADMVLPKSATLEKDGTLTNFDRTVQRVRATVPAMGESKAVHDAIGLLSERMGYG
                     FPVMPASQIMSEIGRLVPSYGGITYSRLERSGTVTPATSFADPGSPILVGG"
     gene            2627..>3099
                     /locus_tag="VHR64_00260"
     CDS             2627..>3099
                     /locus_tag="VHR64_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX3301286.1"
                     /translation="MLTRSKMYCSCSAEYSSAPPNTHTGPVCLGLPGALPVINKAAIE
                     MCVKTGLALNCEIATFSKMDRKNYGYPDLPKGYQISQYDLPLCLQGSLTFESEGAEVT
                     AGITRVHIEEDTGRLVHSRNETGQEISLVDLNRSGVPLMEIVGDPDLRTAQQAGD"
BASE COUNT          575 a          963 c          981 g          580 t
ORIGIN      
        1 accaccgacg aggatcggag agccgggatc cgcaaagctg gtcgccgggg tgaccgttcc
       61 cgatcgctcg agacgcgagt aggtgattcc gccatacgac gggaccagcc ggccgatttc
      121 gctcatgatc tgcgacgccg gcatgaccgg gaagccgtaa cccattcgct cgctgagcag
      181 gccgatcgca tcgtgcacgg ctttgctttc gcccatcgct ggaacggtgg cacgaacacg
      241 ctgcaccgtg cggtcgaagt ttgtcagcgt gccgtctttc tcgagcgtgg cggacttcgg
      301 caggaccatg tctgccttct gggccagtgg cgagttgaag gtatcgaaga cgagcaggaa
      361 ctcgagcttg tcgagcgccg cacccagctc gggatcgatg aactgcaggc gtccggcaac
      421 ggtgccatca atgatcatcg ccttgacgcc gcccgcgttg atcgccttga tcagctcgtc
      481 gcgcgagagg ccccggcttt cgggcagcga ccgcaccggc aggaacccgt tgctggtctt
      541 cgccttctcg gcccagcgat cgagccatgc cgtgccaaac gtctcacgat cgactgccga
      601 atcgacgcga ccgccacccg gcaaatacga cgggtgcgcg cccatctgcg ttgcgccctg
      661 gtagttggcc ggacctcgca ttgccgccac gccgccgccc gcccggccga tgttgccggt
      721 caggaacgca aggttgttgc aggcggccga aatctcggct ggatcgccgt agtagtcgtt
      781 ctccgagacg gagccctggt gcgcgatcgt ctgatagatc agagccgggg ggaagctgtc
      841 ggcgcccgcc gaaatcccgg caccaccgga agcccagagg acggccgctg cttcgatgtc
      901 ggccgccgga acgccggtgt cggccgaaac ctgcgcgatc gagacgttct ggatcgtcgt
      961 cagccactgc tggtagccgg cgacctcgga tccgcccttg gccaggccct tgtcgatgat
     1021 cgcgcgggca atgccattca acagcgtcgc cgccgtgcca ggcttcggct tcaaccagaa
     1081 ctcactgcgg cggcagagcg ggtagtcgtc catcgagatc acgatggtct tggcttcgcg
     1141 gaactggtgg ctgtgataga gccagtagga cgcgaccggc gccgactttc cgatgtccgg
     1201 cccgacgacc atcgcggcct tcacgtcggt gaacacctct tgcatgttgt tcgtgttgga
     1261 aacatcgtca ccgagtccgg cgcggacagc cagctcaacc gagcgctggg caggagtgag
     1321 catccggtcg atattgttcg tgcccatgac cgcgcgagtg aagagctgga gcgcgtagac
     1381 ctcttcgttt gtgctgtcgg ccgaagcgat cgccgcgaac tggtcgccct ggtagtgggc
     1441 gagggtctcg gcggcggagt tgatcgcgtc gtcccaggtg acttcgtaaa ccgttccgtc
     1501 ctcatcgcgg acgtacgggt agaaaatgcg gtcgggattc tgcaggagtt cgtgacccca
     1561 gcgaccggct tcgcaggtgt agccgtgatt gacgccgcgc tcccaaaggt gcgagacccg
     1621 gcgcacgata cccttgttcg attcgacgct gatcgtgcag ccaacatcgc agaaaccgca
     1681 gatcgtctcg gtcgcgtcga gctcccacgg gtggtgcgca aagcggcgat ccgtcagcgc
     1741 gcccaccggg cagacggcga tgcacatgcc gcagcgcgtg caggcggtgt cgtccaggct
     1801 ctggccgtac gccggcgtga tcgcagcatc catgccccgg taggacgact cgatcgccgt
     1861 caccccgatc atctcgtcgc agacccgcgt gcatcggttg cacatgatgc aacggtccca
     1921 cttgtagtcg atgatgtcgc tgaagtggac atacggttgc gcgaacttgg ggcggcgata
     1981 cggattggtg ttgacattgt gcaggtaggt gttgtcctgc aaatagcact cgcccgattt
     2041 gtcgcaggtg gggcagtcaa gcgcgtgatc gatcaggtac atctgaagga tgaactggcg
     2101 cgcatcgatg catggctcgg aatgggtgag gacttccatg ccctcgccga gcggcgtcgc
     2161 gcacgactcg atcagtccac cgcgcttacc aagaatctcg accgtgcaga tgcggcagga
     2221 gccccacggg cggagcagcg gctgatagca gaggttcggg acttcgatgc cggcctcacg
     2281 agcggcctcg agaattgtcg tcccggccag aaccgtgacg tcctgcccgt tgatcttacc
     2341 ggtcaccatt ttgacgtcgg ttcgcgtcgc cattaccgga aatgctgcct tctatgcgtc
     2401 aaagcatcga gcctgattta cggagacgca gcctgaaaaa ggcttacacg cccggggtgg
     2461 atggtaccac agcgggtccc ggtgaatgac tgcggggcaa tctgattcag gcgtgaatga
     2521 cgtactattc gcaagatcac aacatcgggt gatcaccgcc cgatcgcgct tcgttggggg
     2581 tattgtggaa ctcgaaacaa tcattgggct tgaggttcat gcccaggtgc tgacccggag
     2641 caaaatgtac tgctcctgct cggccgagta ttcaagcgca ccccctaata cacacaccgg
     2701 tccggtctgc ctcggcctgc ccggcgcact tcccgttatc aacaaagccg ccattgagat
     2761 gtgcgtcaag accggtctcg ccctgaattg cgagatcgcg acattctcaa agatggaccg
     2821 caagaactac ggatatccgg atctgcccaa gggctaccag atctcgcagt acgatctccc
     2881 gctgtgcctg caggggtcgc tgacctttga gtcagagggc gccgaggtca cggccggaat
     2941 cacgcgtgtc catatcgaag aggacacggg ccggcttgtc cattccagaa atgagaccgg
     3001 ccaggagatc agcctggtcg acctgaaccg gtccggcgtc ccgctgatgg agatcgtcgg
     3061 cgaccccgat ctacgcacgg cccagcaagc gggtgatta
//
LOCUS       DATAOZ010000013         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2112010, whole genome shotgun sequence.
ACCESSION   DATAOZ010000013 DATAOZ010000000
VERSION     DATAOZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112010"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VHR64_00265"
     CDS             complement(<1..209)
                     /locus_tag="VHR64_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301287.1"
                     /translation="MSREDEAARPGPEEADTPRLIEVRREGLRVRVHRRKGTLGGLIE
                     LVVIVALALGLALGIQAWIVKPYEIP"
     gene            complement(338..1441)
                     /locus_tag="VHR64_00270"
     CDS             complement(338..1441)
                     /locus_tag="VHR64_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX3301288.1"
                     /translation="MQPATDIPRVTSSGPQGQRERLLVASRLATVLARVHSVEQVAEI
                     VVRELHDSFDYYLAVVQRLDPDGSLRVVAGAGPLAEELSDFLAQVQPIEVGVNGRVVR
                     TGQPAMVNDTRLDSDYLQRNPCTDPGSELSLPLHVAGSVWGVMNLEQQERGAFTDDDL
                     MLAEIVSSQMGAAIYRCQLLGELEGAFMATLGVIADAVELQDSYTADHANEVADLALA
                     VGRRMGIGGAELDRLRYGALLHDVGKIGVPGEILRKAGPLTPTERELMDEHTAIGARM
                     LEKIPFLAPVAPLVRSAHERDDGGGYPDGLAGDEIPRGAMIICTCDAFHAMTSDRTYR
                     RAMSREEAIAELRASAGTQFDPAVVEALIKTIS"
     gene            1810..2532
                     /locus_tag="VHR64_00275"
     CDS             1810..2532
                     /locus_tag="VHR64_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEX3301289.1"
                     /translation="MNRAARNSGNQKRRMRSTPSILIAGAALFIALGGSAVAAGLIRS
                     EDIAPGAITSKAIRDGAVKPDDLSTTSRDLLQGAPGETGKTGSPGAAGSNGANGPNGA
                     NGVDGKNGTNGINGTNGTDGVDGTIAPLSATKGVTALPTAASLTTVVELAVPTGKYVV
                     LAKTQLSHTGAGDTVECQLKSGGTTIDQIAMKTLPALAAIPASMQAVITVASPSQLSV
                     QCKVKVADGAADFNSLIAIPTG"
BASE COUNT          476 a          857 c          853 g          441 t
ORIGIN      
        1 gggatctcgt atggcttcac gatccacgcc tggatcccca gggccagccc tagggccagg
       61 gcaacgatga cgacgagctc gatcagcccg ccaagtgtcc cctttcgccg gtgaacgcga
      121 accctcaggc cttcacggcg cacttcgatc agtcgcgggg tgtctgcttc ctcaggccca
      181 ggccgtgctg cctcatcctc gcgcgacatg gcgcctcctc tcgtcccttg acatccctac
      241 ccctggcttc ttctgcactg ctatcgctgc ggacgtcagc atagttgcgg ccatctaagc
      301 cggcatggag ccagagcgaa gcttcgggag atctaagtca agaaatagtt ttgatcagcg
      361 cctcgaccac ggctgggtcg aactgggtgc cggcgctggc gcgaagctcg gcgattgcct
      421 cctcgcgact cattgcccgg cggtaggtgc ggtcggaggt cattgcgtgg aaggcgtcgc
      481 aagtgcagat gatcatcgcc ccgcggggga tctcatcgcc agcgaggccg tcggggtagc
      541 cgccgccgtc gtcgcgctcg tgggcagatc ggaccagggg ggcgaccggg gcgaggaagg
      601 ggatcttctc cagcatccgg gcgccgatcg cggtgtgctc gtccatcagc tcgcgctctg
      661 tgggggtcag cggaccggcc ttgcggagga tctctccggg aaccccgatc ttgccgacgt
      721 cgtgcaggag ggcgccgtag cggaggcggt cgagctcggc tccgccgatg cccatccgcc
      781 gcccgactgc cagcgccaga tcagcgacct cgttggcatg gtcggcggta tagctgtcct
      841 gtagctcgac tgcatcggca atcaccccga gggtcgccat gaaggcaccc tcgagctcac
      901 cgagcaactg gcagcggtag atcgccgccc ccatctgact ggagacgatc tcggccagca
      961 tcaagtcgtc gtcggtgaag gcgccccgct cttgctgctc gaggttcatc accccccaga
     1021 ccgagccggc gacgtggagc ggcagcgaca gctcggagcc cgggtcggtg catgggttgc
     1081 gctgcaggta gtcggagtcg agccgggtgt cgttgaccat cgccggctgg ccggtccgca
     1141 cgacgcggcc gttgaccccg acctcgatcg gctgcacctg ggcgaggaag tcgctcagct
     1201 cctcggccag cggcccggcg cccgcgacga cccgcagcga gccatcgggg tcgagtcgct
     1261 gcaccaccgc caggtagtag tcgaaggagt cgtgcagctc tcggacgacg atttcagcca
     1321 cctgctcaac gctatggacg cgggcgagca cggtcgccag ccggctggcg acgagcagcc
     1381 gctcgcgctg gccctgcggc ccgctggagg tgaccctggg aatatcggtg gccggctgca
     1441 cttgcggggt tatcggcctg ctcgtccgtc tctttagcgg ctatctaggt tcaaagctat
     1501 gagcgaagac agcttcgaca tcgtcgggat gaggtccggt ggcacctcgg ttgccggctt
     1561 ccggggcgcc tcgaccgaga atcgcgaggt cacattgctg ctggaagcct gactttaaaa
     1621 gaagaggcga gtcgcgtaag ggctgccgcc ggaagcaatg tgatccgtct tttaaaaaaa
     1681 ggaaggacag atcttcctgg cgctcactat ctggcttgtt cctgtcgatg gggcagcagc
     1741 gggagcaatc gctccgcgct acctcagtac cgcacgagca cgcggtaagc caaaggagca
     1801 gagcccatga tgaatcgtgc tgccagaaac tctggaaacc agaagcggcg aatgcggtcg
     1861 actccttcaa tcctcatcgc cggggccgct ttgttcatcg ccctcggcgg ctcggcggtc
     1921 gccgccgggc tcatccgcag cgaggacatc gcgccgggcg ccatcaccag taaggcgatc
     1981 cgggatggag cggtcaagcc cgatgacctc agcaccacct ctcgagatct ccttcaggga
     2041 gcgccgggtg agacgggcaa gaccggttcc ccaggggccg ccggctccaa cggggccaat
     2101 gggcccaacg gcgctaacgg cgtcgacggg aaaaacggca ccaacggaat caatgggacg
     2161 aacggcaccg acggagttga cggaaccatc gcgccgctat cggcgacgaa aggcgtgacg
     2221 gccctcccca ccgcggcctc gctaaccacc gtggtcgaac tggcggtgcc gacgggcaaa
     2281 tacgtcgtcc tggcgaagac acagctgtcc cacaccggtg cgggcgacac cgtcgaatgt
     2341 cagctcaagt ctggtggcac cacgattgac cagatcgcga tgaagacgct acccgcgctt
     2401 gccgcgatcc ccgcctcgat gcaagcggtg atcaccgtcg cttcgccgtc acagctcagc
     2461 gtccagtgca aggtgaaagt tgccgacggc gcggccgact tcaacagcct gatcgcgatt
     2521 ccaaccggct gatctgcggt ccgaaaagaa aggcagagag gaagcaatgt gatcggcggt
     2581 agacctaaga gtccagcgac cgccgcatcc tcaccttgat tttttaa
//
LOCUS       DATAOZ010000014         6713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5005796, whole genome shotgun sequence.
ACCESSION   DATAOZ010000014 DATAOZ010000000
VERSION     DATAOZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5005796"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VHR64_00280"
     CDS             complement(<1..740)
                     /locus_tag="VHR64_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3301290.1"
                     /translation="MKLSSAMSRLGTETAFEVLVRAKALEAKGKDVVHLEIGEPDFDT
                     PQNIIEAGCEALKSGWTHYGPSAGLPDLREAIAGYLNRSRSTSYAADNIIVTPGGKPI
                     MFFVILALLEPGDEAIYPNPGFPIYESMINFTGAKAVPSPIRESNQFALDVDELEGLI
                     TPKTKLLIINSPGNPTSGVLPRADIERIAELAVKHDLIVLSDEIYSEIIYEGEHVSIA
                     TMPGMAERTIVLDGFSKTYAMTGWRLGYG"
     gene            1069..1296
                     /locus_tag="VHR64_00285"
     CDS             1069..1296
                     /locus_tag="VHR64_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301291.1"
                     /translation="MAGTRWHYYVRRGALSQDALNEIGKEGWELVSVDSGDDEPVFYF
                     KRPHPTLAEAITVEQRERYFSEWGVGQDQTP"
     gene            1293..1679
                     /gene="rbsD"
                     /locus_tag="VHR64_00290"
     CDS             1293..1679
                     /gene="rbsD"
                     /locus_tag="VHR64_00290"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEX3301292.1"
                     /translation="MKRGGIYHPAVNHLLASTGHTDYFTVCDRGFPVPVGPVRIDLAL
                     TDDIPTVLDVVRAVMPEFQVDRVLIATEASTASPDRVQELRVLLDPIPLIAIDHLELK
                     ALAQAGRATIRTADTVPYANIIIVSG"
     gene            1752..3446
                     /locus_tag="VHR64_00295"
     CDS             1752..3446
                     /locus_tag="VHR64_00295"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEX3301293.1"
                     /translation="MSDTEISIRVAGSSPGGRIIGVIDQDHKVHRQTPSFTRELLGSV
                     DADGIVRLEDGQEVGEPIAMITGTRVVTPSLDRATAPVLGFINDSGEVRPSGSDRVLG
                     VVDEANPLGMAFFALAYPRLVAEIDELEVEVRAAKNKLPFRDKIRKRAETLTDSDVLG
                     DVTALVARLARLDSEIEAEVGDRTAAKERLAAEAESLADSTDWKATGERMKALFEEWK
                     TIGSAGKEADEALWARFSSAREQFNTRRKTDFEERQATRGAAKERKEALIAQAEAAVA
                     GESIDYRSVGDKLRSIQAKWKEAGSAGRTDDDELWGRLQAITKPFYEQRSAWYTANQL
                     AKEALIARAEELSSSEDWNGTANALKALQAEWKQVGTAGREADETLWTKFRAANQAFF
                     GQREKVAADRNRNFSDNLSAKMELVKKAQQLRWAPDPFSAANEAKQLQAQWKEIGPVP
                     REKSDEIWKQFREACDKIFANVQSARNRQQSDWTDRLKEAMDRKLEQFGEILRSIDRD
                     QEQLDRLNQQLASKPTPDLEKRVKELSGRIKDKEKRASNIEESLFEIRDKVHGGVD"
     gene            3590..4048
                     /locus_tag="VHR64_00300"
     CDS             3590..4048
                     /locus_tag="VHR64_00300"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX3301294.1"
                     /translation="MTSGIPGTDEAYMRRCIDIARVARQRGDAPVGALIVRDGQSIAE
                     AGERVDSALDIAGHAEVVAIRLACEILGSLDLTGCILYTSTEPCVMCGYAIRQARIGR
                     VVFGAAVPEMGSVTSRYPILIAADVPGWQDPPIIVDGVLGDECASLFSPQ"
     gene            4118..5455
                     /locus_tag="VHR64_00305"
     CDS             4118..5455
                     /locus_tag="VHR64_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3301295.1"
                     /translation="MASESFDVDPDVMDRVLSAIDKRSDEIVQFASMLIQQPSINPDL
                     EPNEQAERPAQEWLRDQFLAMGSFSGIDFWEVERNRPNVVAVRAGEGGGRSLIWSAHT
                     DVVPVTPEQAEQWEGAGPFSGEVRDGWLWGRGASDMKGAIAAYVMAAQILHDEGVRLK
                     GDLRLSQAIGEEAGRRDIGCNTVLERGHRADMAIFPEPSNFAIYPTVKGELYFRLTVP
                     GKSTHICNRHLVAQPLPYGVERPGVSAIDNMLKYQLAILELEKQWNLWRSNEHVPAGG
                     MFININTMQAGTSLTSVPDSADATGSLLFNPDLTGEEVMAEIRATIDRVTQGDYWLRE
                     HPPVLDLPLDASSAAPWIKEPVNLAHDHPIIGTIQAASEAAIGQKPDVTISPFVCDAN
                     FWYPLGQDCLIWGIGDPSWGIHGTNEKLPVADLINGTKAFAAATMAWCGVASA"
     gene            5652..6362
                     /locus_tag="VHR64_00310"
     CDS             5652..6362
                     /locus_tag="VHR64_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301296.1"
                     /translation="MSSQPPKDFLALSHGHEWVDFYESFDKLVQDHLSRSSELLRKAM
                     SLPEVADREVAQVRDDLESRLTAERAQNQATLSTLKQEITSSHQQAASLAQLVGSLMA
                     QLDRLNGNVESALAKIAENEAIAPSGAAQIESGPPVHVEAPVASAPESASTEWSAEAA
                     EEQSEPVAESNEDWSQLTDEAKDESADEIDLSALAAMEEATNEAEDSDEAEAEVVAAN
                     NGSERTRPHWLSMARSGQ"
BASE COUNT         1265 a         2000 c         2335 g         1113 t
ORIGIN      
        1 ccgtagccga gccgccagcc ggtcatggcg taggtcttgg agaagccgtc gaggacgatc
       61 gtgcgctcgg ccatgccggg catggtcgcg atcgagacgt gctcgccctc gtagatgatc
      121 tcgctgtaga tctcgtcgga gaggacgatc aggtcgtgct tgacggcgag ctcggcgatg
      181 cgctcgatgt cggcgcgggg caggacgccg ctggtcgggt tgcccgggct gttgatgatc
      241 agcagcttcg tcttcggcgt gatcagccct tccagctcgt cgacgtcgag cgcgaactgg
      301 ttggattcgc ggatcggcga cggcacggcc ttggcgccgg tgaagttgat catcgactcg
      361 tagatgggga agcctgggtt ggggtagatc gcctcgtcgc cgggctcgag cagggcgagg
      421 atgacgaaga acatgatcgg cttgccgcca ggggtgacga tgatgttgtc ggccgcgtag
      481 ctcgtgcttc tggaacggtt cagatagccg gcgatcgctt cccggagatc gggcaggccg
      541 gcggagggac cgtaatgggt ccagccggat ttcagggcct cgcagccggc ctcgatgatg
      601 ttttggggcg tgtcgaagtc cggctcgccg atctcgaggt ggacgacgtc cttgcctttg
      661 gcttcgaggg ccttggcgcg gacgaggacc tcgaaggcgg tctcggtgcc gaggcgggac
      721 atggcggatg acagcttcat tgcgtgctcc agggtgggaa tgacctcacg tttcgactgc
      781 gcaagcatac ccgcccggag gtcggtagaa tcgggtcaga ggcgcgaagg cgcgaaggcg
      841 cggagttagc ggggacgaag tacgcttcga atggcagaag cgcgggtcga catcgcccgg
      901 cgctaaagca ccgggctaaa caacataagc cagctaaagc aggctagagg tcggccgatt
      961 caatttaacg gcgcgttgga ttggaaccgg tcaatgcctc acgggcgatc gcggggcacg
     1021 gaggcacccg cactacattt tcggagctga gaatggagga tcgttgatat ggcggggacg
     1081 aggtggcatt actacgttcg gcggggtgcg ctgtcgcagg atgcactgaa tgagatcggc
     1141 aaggagggct gggagttggt ctcggtcgac tccggtgacg acgagccggt cttctacttc
     1201 aagcggccgc atccgacgct ggccgaggcg atcacggtcg agcaacgtga gcgctacttc
     1261 agcgaatggg gcgttgggca ggatcagacg ccgtgaagag gggcgggatc tatcacccgg
     1321 cggtcaacca cctgctggcc agcaccggcc acaccgatta cttcactgtc tgcgaccgcg
     1381 ggtttccggt tccggtcggc ccggtgcgga tcgacctggc cctgaccgac gatattccga
     1441 ccgttctgga tgtcgtccgg gcagtgatgc cggaatttca ggtcgaccgc gtgctgatcg
     1501 cgaccgaggc atccacagcg agcccggacc gcgtacaaga actacgagtg ctacttgatc
     1561 cgatcccgct gatcgcgatc gaccacctcg aactgaaggc gctcgcacag gcaggacgag
     1621 cgacaattcg gactgcggat accgtgccct acgcgaacat aatcattgtt tcggggtaga
     1681 gccggttaca atggcgccta gcaggtagcc gtgtgtcttc agacaggtac tttctccgga
     1741 gcaacgtttc agtgtcggat acggaaatct caattcgcgt cgcgggctcc tcgccgggcg
     1801 ggcgcatcat cggcgtgatc gatcaggacc acaaagtgca ccggcagacg ccgtcgttca
     1861 ccagggagct gctcggctcg gtcgacgccg acggtatcgt gcggctcgaa gacgggcagg
     1921 aggtcggtga gccgatcgcc atgattaccg ggacgcgcgt cgtcacgcca tcgctggacc
     1981 gcgcgaccgc tccggtgctc ggcttcatca atgattcggg cgaggtgcgg ccttcgggat
     2041 cggatcgcgt cctgggcgtc gtcgacgagg cgaatccact cgggatggcc ttctttgccc
     2101 tcgcctatcc gcgcctggtc gccgagatcg acgaactgga agtcgaggtg cgcgcggcca
     2161 agaacaagct gccgttccgc gacaagatcc ggaagcgcgc cgagaccctg accgactccg
     2221 acgtgctggg ggacgtgacg gcgctggtcg cccgcctggc ccggctcgac tcagagatcg
     2281 aggcagaggt cggcgatcgc acggccgcca aggagcggct ggctgccgag gcggaatcgc
     2341 tggccgattc gaccgactgg aaggccacgg gcgagcgcat gaaagcgctc ttcgaggaat
     2401 ggaagacgat cggcagcgcg ggcaaggagg ccgatgaggc gctctgggct cgcttctcct
     2461 ccgcccggga gcaattcaac acgcggcgca agaccgattt cgaggagcgg caggcgacgc
     2521 ggggcgcggc caaggagcgc aaggaggcgt tgatcgccca ggcggaggcc gccgtcgccg
     2581 gcgagagcat cgactatcgc tcggtcggcg acaagctgcg cagcatccag gccaagtgga
     2641 aagaggcggg ctcggctggt cggaccgacg acgacgagct ctggggtcgg ctgcaggcga
     2701 tcacgaagcc cttctacgag cagcgttcgg cctggtatac ggcgaaccag ctggccaagg
     2761 aggccttgat cgccagggcc gaggaactga gctcgtccga ggactggaac ggcaccgcaa
     2821 acgccctgaa ggcactccag gcagagtgga agcaggttgg cacggccggt cgcgaagccg
     2881 acgagacgct ctggacgaag ttccgggcag ccaaccaggc cttcttcggt cagcgcgaga
     2941 aggtcgcggc ggatcgcaat cgcaatttct cggacaacct gtcggcgaaa atggagctgg
     3001 tcaagaaggc gcagcagctc cgctgggcgc cggatccgtt cagcgcggcc aacgaggcca
     3061 agcagctcca ggcgcagtgg aaggagatcg gcccggtccc gcgggagaag tcggacgaga
     3121 tctggaagca gttccgcgag gcgtgcgaca agatcttcgc caatgtccag agcgcgcgga
     3181 atcgccagca aagcgactgg accgatcggc tcaaggaggc gatggaccgc aagctggagc
     3241 aattcggcga gattctgcgc tcgatcgatc gtgaccagga gcagctcgac cggctcaacc
     3301 agcagctagc atccaagccg acgcccgacc tcgagaagcg ggtcaaggag ctaagcggcc
     3361 ggatcaagga caaggagaag cgggcctcga atatcgagga gtccctcttc gaaatccgag
     3421 acaaggtcca cggcggggtc gactagatca ccgactgacc ggcgacagag tccaaacatt
     3481 gtgccggcct tcgtggccgg catttctttg atcacgggcg agattcatgg attcaagcag
     3541 ccatcgtgcg gcgtggcagg tgagatttcg ccaagcatcg gagtgagttg tgactagcgg
     3601 aattcccggc acggacgagg cgtacatgcg gcgctgcatc gacatcgccc gggtcgctcg
     3661 acagcgtggc gatgccccag tcggtgcgct gatcgttcgc gacgggcaga gcatcgcaga
     3721 ggccggcgag cgagtcgact cggcgttgga tatcgccggg catgcggaag tcgtcgcgat
     3781 tcgactggct tgcgaaatac tgggctcgct cgacctgacc ggctgcattc tctacaccag
     3841 caccgaaccc tgcgtcatgt gcgggtacgc gatccggcaa gcgcgaattg gccgcgttgt
     3901 cttcggagcg gccgtgcccg aaatggggag cgtcacctcc cgctacccga tcctgatcgc
     3961 agcggacgtg cccgggtggc aggatccacc gatcatcgtt gatggagtgc tgggagatga
     4021 atgcgcctcg ctcttctctc cgcagtgacg aaccacccgc aaccctttcc cggtacactg
     4081 ccgacgcgac acattcatgc aggccgtgga ggttgcgatg gcgtccgagt cgtttgatgt
     4141 tgaccctgat gtgatggacc gggtgctgag cgcgatcgac aagcggtcgg acgaaatcgt
     4201 ccagttcgcc tctatgctga ttcagcagcc gtcgattaac ccggatctcg agccgaacga
     4261 gcaggccgaa cggccggccc aggagtggct gcgggatcag ttcctggcga tgggttcctt
     4321 ctccggcatc gacttctggg aggtcgagcg gaaccggccg aatgtggtcg cggtccgcgc
     4381 aggcgagggc ggcgggcggt cgctgatctg gtcggcgcac accgacgtcg tgccggtcac
     4441 acctgagcag gccgagcagt gggagggcgc gggtcccttc tcgggcgagg tgcgggacgg
     4501 ctggctgtgg ggacggggcg cgtcggacat gaagggtgcg attgcggcgt acgtgatggc
     4561 cgcgcagatc ctgcatgacg agggcgtgcg gctgaagggc gatctccggc tctcgcaggc
     4621 gatcggcgaa gaggctgggc ggcgcgacat cggctgcaac acggtgctcg agcgcggcca
     4681 ccgggccgac atggcgatct tcccggagcc gtccaacttc gcgatctacc cgacggtcaa
     4741 gggcgagctc tatttccgac tgaccgtgcc gggcaagtcg acccacatct gcaaccggca
     4801 cctggttgcg cagccgctgc catacggcgt cgagcgaccg ggggtgagcg cgatcgacaa
     4861 catgctcaag taccagctcg caatcctcga gctcgagaag cagtggaatc tgtggcggtc
     4921 gaacgagcac gtgccggcgg gcggcatgtt catcaacatc aacacgatgc aggccggcac
     4981 ctcgctgaca tcggtaccgg actcggcgga tgcgaccggc tcgctcctct tcaacccgga
     5041 cctcaccggc gaggaggtga tggccgaaat ccgggcgacg atcgaccgcg tgacgcaggg
     5101 cgattactgg ctgcgcgagc atccgccggt gctggatcta ccgctcgatg cgagcagtgc
     5161 cgcgccgtgg atcaaggagc cggtcaacct cgcgcacgat cacccgatca tcggtacgat
     5221 ccaggcagcg agtgaggcgg caattgggca gaagccggat gttacgattt ctccgtttgt
     5281 ctgcgacgcc aatttctggt acccgctcgg ccaggactgc ctgatctggg gcatcggcga
     5341 tccgagctgg ggtatccacg gcacgaacga gaagctgccg gtggcggacc tgatcaacgg
     5401 gacgaaggca ttcgcggcgg cgaccatggc ctggtgcggg gttgcctcgg cctgagccgg
     5461 tccgtttggc ccgaacgaac ggctccgcca ttggtaggac aacgctactt ccgggctaag
     5521 tgatgccgta gtcacaatac gggttgcgcc atagagtagg gttgaattca gccggggaag
     5581 ccggtcagac caaggcgttc atggaaacgc cgcgtattca atcgtcgctg gacgatggga
     5641 gcacctgagg catgagcagt caaccgccca aggattttct cgcgctcagt catgggcacg
     5701 agtgggtcga tttttatgaa tcgttcgaca agctggtcca ggaccacctg tcccgttcgt
     5761 ccgagctgct gcgcaaggcg atgtccctgc ccgaagtggc ggatcgggaa gtcgcgcagg
     5821 tccgtgacga cctcgagagc cggctgaccg ccgagcgcgc gcagaaccag gcgaccctgt
     5881 ccacgctcaa gcaggagatc accagcagcc accagcaggc ggcgtcgctt gcacagctcg
     5941 tgggttcgct gatggcccaa ctcgatcggc tgaacggtaa cgtcgaatct gcgctggcca
     6001 aaattgccga gaacgaggcc atagccccat ccggtgcggc tcagatcgag tccggccctc
     6061 ctgttcatgt cgaggcaccg gtcgccagcg cgccggagtc tgcgtcgacg gagtggtcgg
     6121 ctgaggccgc agaggagcag tccgagccgg ttgcggagag caacgaggac tggtcgcagc
     6181 tgaccgacga ggcgaaggac gagtcggctg atgagatcga cctctcggcc ctggcggcga
     6241 tggaagaggc gacgaacgag gcggaggact ccgacgaggc tgaagctgag gtggtagccg
     6301 ccaacaacgg ctcggagcgc acccggccgc attggctgag catggcccgc tccggccagt
     6361 agacccgacc cacaaaacac gcaagctcaa ccgcccgggt gatcgatgat cacccgggcg
     6421 gttctgtgtt catcgggcaa acgtggcgct gcccgccaaa tgcagagtcc agacgggttg
     6481 tccggtcggt gacgtggcgc ggggttacca tgccccgccc ggacccgatg ccggccacgg
     6541 cggtggggga acgtgccccg ttacctgatc gtctccatga gccgggcata gggatgcacc
     6601 aagcggggcg tgtacctctc tcagactctc tcctcaaggc aagcgagcat ccccgtgcta
     6661 tgttccggcg ccggtcaaga catatgcacg ccgcagtagc tgtcgggcgc atc
//
LOCUS       DATAOZ010000015         5048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5532368, whole genome shotgun sequence.
ACCESSION   DATAOZ010000015 DATAOZ010000000
VERSION     DATAOZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5532368"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VHR64_00315"
     CDS             <1..962
                     /locus_tag="VHR64_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3301297.1"
                     /translation="VVFHSPTTVFSPENVAAVHQLVADLQQDQRVVRVEGFAAFPQQV
                     TDEQASALVQVQRRAAASGLGARFNQIANNQTAMVLVYTESYPNSVESKELLAKIREY
                     PVGGDMTMLVDGGTAEIVDVVSAMYGAFPLAVGFVLLATYIVLFLLFHSVFLPLKAIV
                     MNTLSLLASYGALVWVFQDGHLERLLRFESLGYVEASLPIIMFCILFGLSMDYEVFLL
                     SRVREEWERTGDNTEAVALGLQRSGRIISSAALIVVVVAISFVSADVILVKALGFGIA
                     LAVFLDATIVRALLVPATMRLMGHWNWWRPSFLARLAIPDLHE"
     gene            1305..1559
                     /locus_tag="VHR64_00320"
     CDS             1305..1559
                     /locus_tag="VHR64_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301298.1"
                     /translation="MFTSRHGRFEITLSQDRDGWLVQYTSTGRLFGPREVIYSARHTV
                     ARHAAWDVMCRVIRATSDEQLGVDVGKKAAAWLQARRSVC"
     gene            1569..2426
                     /gene="mutM"
                     /locus_tag="VHR64_00325"
     CDS             1569..2426
                     /gene="mutM"
                     /locus_tag="VHR64_00325"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEX3301299.1"
                     /translation="MPELPEVETARRIIESTLVGAHLIATEVRLPKMLRYSDLPDIDV
                     LLGNQLISARRRAKVLITDWSDDLAMLFHLKLSGQVSLQRDDERRTAGHPVPDPDGAY
                     PHKTTHISFDFDTGWTLHLSDVRQFGWIRIMPADAVDGFVAAQSFGPEAVGPEGITAE
                     QLGASLARRSIPVKSALLDQTVVAGIGNIYVDEALHRARIHPMTPAKDVPPDALPTLV
                     DAIQWAIGKGIEQGGAKIIHNKAYPIDGFPEVHARQGEQCPVCGDEIIKIRVGQRGTY
                     LCPTCQPEP"
     gene            complement(2427..3491)
                     /locus_tag="VHR64_00330"
     CDS             complement(2427..3491)
                     /locus_tag="VHR64_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3301300.1"
                     /translation="MRSESWTFLKNLIETPGPSGYERDVAAVWRKEAEGFADEVDRDL
                     TGNSFAWLRSSDPNAPTVLVEGHIDEIGVQITHIDPEGYIWFDGIGGWDAQVLTGQRI
                     KFTCADGPVIGVVGRKAAHLLKAEDRDKAVKIEDLWIDIGAGSREAALKRVAIGDAGV
                     VDAPFTGIGDGLIVSRAIDNRSGAFVALETLRLLSENRPWANVVALAAAQEEISYAGA
                     FTATFRTDPTVAIAIDVTHATDYPGADKKRNDEVALGSGPVFTRGSSASPVVFERLVA
                     AANSSEISFTVQGSARQTWTDADAMVRSGRGPATGIVSIPTRYMHSPNETIAVSDLGA
                     CAQVLAEFVRRIDKETDFRP"
     gene            complement(3427..3690)
                     /locus_tag="VHR64_00335"
     CDS             complement(3427..3690)
                     /locus_tag="VHR64_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301301.1"
                     /translation="MTYAIVTAILVLALVAVVIALIVDTDEAPAEEVIQIQLPSQPLP
                     YFDAGPSIPAPPYVVNPEESQAREIRVVDVPEEPDRDSRPFGV"
     gene            complement(3687..4484)
                     /locus_tag="VHR64_00340"
     CDS             complement(3687..4484)
                     /locus_tag="VHR64_00340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3301302.1"
                     /translation="MRPNDPAYSRYARHYDKIGQRRFGESSATDLLRILELQGERPQS
                     VLDLACGTGAATIVFARSGLETTGLDIAHAMIEEASAAAKSAGVSIRWVLADMTAYEA
                     DRQYDLCTCFYDAVNYLTDLREFSAFARCAYDALNPGGTFAFDINTRRKLAEHWGQMT
                     LIAANDSDRFLTYRSWFDERHGVSPLIITGFERREDGAWDRFDEEHVETAFAIDDLRS
                     KLESAGYEQIQVIDWREGDISDLAPGTEDAFRVLFMARKPATAQEES"
     gene            complement(4486..>5048)
                     /locus_tag="VHR64_00345"
     CDS             complement(4486..>5048)
                     /locus_tag="VHR64_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301303.1"
                     /translation="TAATDAFCQANTRCTVDESFERFAPVLERATADGVKVRGAVSVA
                     FHCPYSGPVDPEAAVRVAERLLQIGCAEISIADTIGRASPGDVRRLLDVAFPRLPVER
                     VAMHFHDTSGQALDNIRLSWDAGITRFDSSVGGLGGCPFAPGAPGNVASEHVVGMFAG
                     QGIRTGIDLDRLTETGSWIRSVLGKG"
BASE COUNT          907 a         1569 c         1589 g          983 t
ORIGIN      
        1 tggtcgtctt ccattcaccg accactgttt tcagtccgga gaatgtcgcg gccgtccacc
       61 agcttgttgc cgatcttcaa caagaccagc gagtcgtgcg cgtcgagggg ttcgcggcgt
      121 tcccgcagca ggtcacggac gagcaggcgt cggcgctggt gcaggtgcag cggcgagccg
      181 cggcgagcgg gctcggcgcc cggttcaatc agatcgccaa caaccagact gcgatggtgc
      241 tggtctatac cgagtcctac ccgaacagcg tcgaaagcaa ggagctgctc gccaagattc
      301 gcgagtatcc ggtcggcggc gacatgacga tgctggttga tggcggcacc gccgagatcg
      361 tcgatgtcgt cagcgcgatg tacggcgcat tcccgctcgc ggtcggattc gtcctgctgg
      421 cgacctacat cgtcctcttc ctgcttttcc actcggtgtt cctgccgctg aaggcgattg
      481 tgatgaacac cctctcgctc cttgcgtctt atggggcgtt ggtctgggtc ttccaggacg
      541 gccatctgga gcggttgctg cgattcgaat ctctcgggta tgtcgaggcc tcgctcccga
      601 tcatcatgtt ctgcatcctg ttcggcctga gcatggatta cgaggttttc ctgctgagcc
      661 gcgttcggga agagtgggag cgcaccggcg acaacaccga ggcggtggcg ctcgggttgc
      721 agcgtagcgg ccggatcatt tcgagtgccg cgctgatcgt cgtggtggtc gcgatctcgt
      781 tcgtgtcagc agacgtgatt ctggtcaagg cgctcggatt cggcatcgcg ctggccgttt
      841 tccttgacgc cacaattgtc cgcgcgctgc ttgttcccgc gacgatgcgg ctgatggggc
      901 actggaattg gtggcgtccg tcgttcctcg cgaggctggc gattcccgat cttcacgagt
      961 gaatgttgtc gtcgacaaca tcccttgttc ccatccgatc gggcattgca cggtgggacg
     1021 attttcgaca tttgttcgaa ttgttgacgc gccttttgct gtgctacatt gtcatggcag
     1081 ctgttgagag atccggtcat gtacccacgg ttggggtggg catgccgtct cgttgacagc
     1141 cgcgcttgac tatctgtcag aaaccgtcgc gttcggttgc ctggaagggc tttgggcaca
     1201 tggatgtagt gatctcctct ggctggcgta cgtcttctac cacgatcggt ctcgaccccg
     1261 ttgacgaagg aatcacccga cccattgcca gtacgcctgc ggacgtgttc acaagccgac
     1321 acggccgctt cgagatcacg ttgagccagg atcgagatgg ctggttggtc cagtacacct
     1381 ccactggccg cctattcggc ccgcgcgaag tcatttacag cgcccgccac accgtcgccc
     1441 gtcatgccgc gtgggacgtc atgtgccgcg tcattcgggc taccagcgat gagcagctcg
     1501 gcgtcgatgt cggcaagaag gccgcagcct ggctgcaggc gaggcgctca gtctgctaat
     1561 agttccgcat gccggaactg cctgaagtcg aaaccgcccg acgaatcatc gaatccacgc
     1621 tggtcggggc gcacctcatt gcgaccgagg tgcgccttcc caaaatgctg cggtattcgg
     1681 acctcccgga tatcgatgtg ctgctcggga accagctgat ctcggcccgg cggcgagcca
     1741 aggtgctgat caccgattgg tccgatgacc tcgcgatgct ctttcatctc aagctctcgg
     1801 ggcaggtcag tctgcagcga gacgacgagc gccgaaccgc cggccacccc gtgccggacc
     1861 cggacggtgc ctacccccac aagacgaccc acatctcgtt cgatttcgac accggttgga
     1921 ccctccacct gagcgacgtt cgccagtttg gctggattcg gatcatgccg gccgatgcgg
     1981 tcgacgggtt cgttgccgcc cagtcatttg ggcccgaagc agtcggaccg gaggggatta
     2041 ccgccgagca gctcggcgcg agcctggcga gacggtcgat tccggtcaaa tcagcgctgc
     2101 tggaccagac cgtcgtcgcc gggatcggca atatctacgt cgatgaggct ctgcaccgag
     2161 cgcggatcca cccgatgaca cctgccaaag atgtgccacc cgacgcgctg ccgacgctcg
     2221 tcgatgcgat tcagtgggca attggcaagg ggatcgaaca gggcggcgcg aagatcatcc
     2281 acaacaaggc gtacccgatc gatggttttc cggaagtcca cgcgaggcag ggcgagcagt
     2341 gcccggtctg cggcgacgag atcattaaga tcagggtcgg acagcgcggc acctacctct
     2401 gcccgacatg ccagcccgag ccctgactac gggcggaaat cggtctcctt gtcgatcctg
     2461 cggacgaact cggccaggac ttgagcacac gcgcccagat cgctgacggc gatcgtctca
     2521 ttgggcgaat gcatgtaccg ggttgggatt gagacgatgc cggtcgcggg accgcggcca
     2581 gatcgcacca tcgcgtcggc gtcggtccag gtctgccggg ctgagccctg tacggtgaag
     2641 ctaatctcgg atgagtttgc ggcggcgact agtcgttcga aaacgaccgg acttgcggat
     2701 gacccgcgag tgaataccgg accactcccg agcgccactt cgtcgtttcg cttcttgtca
     2761 gcgcccgggt agtcggtcgc gtgggtaaca tcgatagcga tggcgaccgt gggatcagtc
     2821 ctgaacgtgg cggtaaaagc gccggcgtat gaaatctcct cctgtgcggc ggcaagagca
     2881 acgacgttcg cccacggcct attctccgac agaagtcgca gggtctccag cgcgacgaag
     2941 gcaccagagc gattgtcaat ggcacgggag acgatcaggc cgtcgccgat ccccgtaaac
     3001 ggagcatcga ctacgccggc atcaccgatg gcgacgcgct tgagggcagc ctcgcgggag
     3061 ccggccccga tgtcgatcca gagatcctcg atcttgacag ccttgtctcg atcttccgcc
     3121 ttcaggaggt gcgccgcctt gcgaccaacg acaccgatta cggggccgtc ggcgcacgtg
     3181 aacttgatgc gctggccagt aaggacctgc gcatcccagc cgccgatgcc atcgaaccag
     3241 atgtagccct cggggtcgat gtgcgtgatc tggacgccga tttcgtcgat gtggccctcg
     3301 acgaggactg tgggcgcgtt cggatccgat gaccggagcc aggcgaagga atttccggtc
     3361 aggtcgcgat cgacttcgtc ggcaaagcct tcggcctcct tgcgccagac tgcagcgacg
     3421 tcccgctcat accccgaagg gccgggagtc tcgatcaggt tcttcaggaa cgtccacgac
     3481 tcggatctca cgggcttggc tctcctctgg attcacgacg tacggcggag ccgggatgct
     3541 gggtccagca tcaaagtatg gcaatggttg ggatggaagc tgaatctgga taacttcctc
     3601 ggcgggagct tcgtcagtat cgacgatcag ggcgatgacg accgcgacca gggccaggac
     3661 gagaattgcg gttacgatgg cgtacgtcat gattcctcct gcgccgtcgc cggcttccgg
     3721 gccataaaca gtacccgaaa ggcatcctct gttcccggcg cgaggtccga gatgtcacct
     3781 tcgcgccagt cgatcacctg gatctgctcg tacccggctg actccagctt cgaccgcaga
     3841 tcgtcgatgg cgaaggccgt ctcgacgtgc tcctcgtcga agcggtccca cgcaccgtcc
     3901 tcgcggcgtt cgaacccggt gatgatcagc ggagacacgc catgccgctc atcgaaccag
     3961 ctccggtagg tgaggaagcg gtcgctgtcg ttggcggcga tcagggtcat ctggccccag
     4021 tgctcagcaa gcttgcggcg cgtgttgatg tcgaacgcga acgtgccacc cggattgagc
     4081 gcatcgtacg cgcagcgggc gaaggcactg aactcgcgca ggtctgtcag gtaattcacc
     4141 gcgtcataga agcaggtaca gagatcatat tgacgatccg cctcgtacgc agtcatgtcg
     4201 gccagaaccc agcggatcga aacccctgct gacttggctg cggcactcgc ctcttcgatc
     4261 atggcatgcg cgatgtccag gccggtcgtt tcgagtccgc tacgagcgaa gacgatcgtt
     4321 gccgcgccgg ttccgcaggc gagatctagg actgattgcg gtcgctcgcc ttgcagctcg
     4381 aggatgcgca ggagatcggt ggccgagctt tcgccgaatc gccgctggcc gatcttgtcg
     4441 tagtggcgcg cgtaccggga gtaggcaggg tcgttcggcc gcatctcaac ccttgcccag
     4501 caccgatcgg atccaggagc cggtctcggt caatcgatcg aggtcaatgc cggtgcggat
     4561 tccctgcccc gcgaacatac ccaccacgtg ctcactggcg acattccctg gcgctcccgg
     4621 cgcgaacggg cagccgccga gaccgccgac ggaggaatcg aatctggtga tgccggcatc
     4681 ccagctaagc ctgatattgt cgagtgcctg ccccgaggtg tcgtggaaat gcatcgccac
     4741 tcgttcaacc gggaggcgtg gaaatgcgac gtccaacaat ctccgaacgt cgcctgggga
     4801 ggcgcgccca atcgtgtcgg cgatcgagat ctcagcgcag ccgatctgaa gcagacgctc
     4861 ggcgaccctc acggcagcct ccggatcaac cggaccgcta tatggacaat ggaacgcaac
     4921 cgagaccgcg ccgcgcacct tcacgccgtc cgcggtagca cgctccagga ccggggcgaa
     4981 ccgctcgaac gactcgtcga cggtgcatcg ggtatttgcc tggcagaagg catcggttgc
     5041 agcggtaa
//
LOCUS       DATAOZ010000016         3501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7900027, whole genome shotgun sequence.
ACCESSION   DATAOZ010000016 DATAOZ010000000
VERSION     DATAOZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7900027"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VHR64_00350"
     CDS             complement(<1..234)
                     /locus_tag="VHR64_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3301304.1"
                     /translation="MADVLVAGAINTDLVARLPRAPEAGETVTGSTFNIFGGGKGANQ
                     TLASARSGAATAILGSLGRDDFGSQRLADLRDEG"
     gene            complement(256..2829)
                     /locus_tag="VHR64_00355"
     CDS             complement(256..2829)
                     /locus_tag="VHR64_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX3301305.1"
                     /translation="MATLTGSASKQPIAGPEYVAEGLALSLHAVLVLSVVGAMNRVAL
                     GDRIGVLGPLALMGLVLGYLLSQTRAQDLVAHSIALWTGAMAAVLLVTLETAGPVEIV
                     RSKGAAFVDLVTGVSQSLVRDDTSSVTDAQLLVVLGVTAWLLAYSSAWVIYRRGWFGL
                     GVAVPASILLASIRVDNRNGGWPLALFIFAAIGLAARHAIAANSMRWARRNMMTGPGL
                     ASRFLVAALPVAVAAVVAALLVNPGIHRAMSVPYRETAQQGWEQLRDKVESVIGKTGP
                     GGGSYASFPDNFEIGGNIDLSNEVVARVQSDSGHYLALRRYDVYDGQGWSSGVQSTFK
                     LDGDASNIRVTNVIFADSQSVALSGAMTGDREPESALITVERPKDDLIFTIETFSSAS
                     RQVYAVLGWERLNRTEIDVDAVDLASIPVDLQSLVRTVRNAPFEVDRASGKVTLTDPS
                     VQEAFDRARQRLAAYPVIADLTVGDQGQLVIVVSGRIPNYDDIEALFTSDAIHANTDY
                     RVVGLSSTASPDQLASASTDYPAWIANRYLQLPDSVTERTRNEAQRVVIQAGATNAFD
                     KVWAVQEYLRSNFTYELNSPRPSGDQDWVDYFLFDHQAGRCEQFASAMIVMVRSLGIP
                     ARLVSGYNYSGETDAQGEVVYRENQAHTWVEVFFPEYGWVPFEPTTNQAEFTYGTDNP
                     DQASETPEATDASPTPEPTVESTPQAEPTPDATPIPPQLSKDSGDNGSRTARLIQIAA
                     ILLALFCFGTLGFMGLMWQWRVRGLPPAGGLFARLQRVGAWFGVRSADSTTPYEFGRA
                     LTTVLPGTEAPIRSITNAYYAEQFDPASSNQESISAARTGWKQLRRNLLRWRIRRPKP
                     R"
     gene            complement(2833..>3501)
                     /locus_tag="VHR64_00360"
     CDS             complement(2833..>3501)
                     /locus_tag="VHR64_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3301306.1"
                     /translation="VQGDSINRVSWTATARAGRLMVKEFENDPTADVWVVLDLYTPPT
                     DGFSHGARFASDDPLNWLSNEFEYRVMLAASLVRRSLALGRSVGLIMNAEQPIVLPPE
                     RSDRQYVRILELLAVVTGRDDATLVELLTSLTGRFRKDSVLLTVTSFADPNLVALLAT
                     MRRRRISSEVHFVDAHMNPDVTITNFLEDLHREHVVGYRMSRDDHPTSALQISNGFAG
                     AWVR"
BASE COUNT          636 a         1110 c         1143 g          612 t
ORIGIN      
        1 gccctcgtcc cgcaagtccg ccaatcgctg ggagccgaaa tcgtctcgtc cgagcgagcc
       61 caggatcgca gttgcggcgc cggagcgagc tgaggcgagc gtctgattcg cgcccttgcc
      121 tccaccgaag atgttgaagg tcgagccggt gaccgtttca cccgcttccg gggcgcgggg
      181 caatcgcgcg acgagatccg tgttgattgc gccggcaaca agcacgtcag ccatctgcgg
      241 tgattctccg tttgatcagc gaggtttcgg acgccgtatg cgccatcgta atagattgcg
      301 ccgcagctgt ttccagccgg tccgggcggc cgaaatcgat tcctggttcg aggatgcggg
      361 atcgaactgc tccgcgtagt aggcgttcgt gatcgaccgg atcggcgcct cggtgccagg
      421 caggaccgtc gtcaatgcgc ggccgaattc gtacggcgtg gtcgaatcgg cgcttcgcac
      481 gccgaaccat gcgccgacgc gctgaagccg ggcaaagagg ccacctgccg gcggcagccc
      541 gcgcacgcgc cactgccaca tgagacccat gaaaccgagg gtgccgaagc agaacagtgc
      601 caggaggatt gctgcgatct ggatcaggcg tgccgtccgc gagccattgt cgccggagtc
      661 tttcgagagc tgcggcggga tgggcgtcgc atccggtgtc ggctcggcct ggggtgtgga
      721 ctccacggta ggttccggtg taggcgatgc atcggtcgct tcaggcgtct ccgacgcttg
      781 gtcgggattg tcagttccgt aggtgaactc ggcctggttc gtcgtcggct cgaacgggac
      841 ccagccgtat tccgggaaga agacctcgac ccaggtgtgc gcctgattct cgcggtagac
      901 gacctcaccc tgcgcatcgg tctcgcccga gtagttgtag ccactgacca gtcgcgcggg
      961 gatcccgagt gatctgacca ttacgatcat ggccgacgcg aactgctcgc agcggccggc
     1021 ctggtgatcg aagaggaagt agtcgaccca gtcctggtcg cctgatggtc gcggactatt
     1081 cagctcatac gtgaagttgg agcgcaggta ctcctggacc gcccagactt tgtcgaacgc
     1141 attggtcgcg ccggcctgga tcaccacgcg ctgcgcctcg ttgcgggttc gctccgtgac
     1201 cgaatcgggg agctgcaggt atcggttcgc gatccaggcc gggtagtcgg tcgacgccga
     1261 tgccaactgg tccgggctcg ccgtgctgct caaacccacg accctgtagt cggtgttcgc
     1321 atggatggcg tcgcttgtga agagtgcctc gatgtcgtcg tagttcggga ttcggcccga
     1381 gacgacgatc acgagctgac cctggtcgcc gacggtgaga tcggcaatca ccgggtaggc
     1441 ggcgagtcgc tgccgggccc gatcgaatgc ttcctggacg ctcgggtcgg tcagtgtgac
     1501 tttgcctgat gcgcggtcga cctcgaacgg cgcgttgcgt actgtgcgga cgaggctctg
     1561 gagatcgacc gggatcgacg ccaggtcaac cgcgtcgaca tcgatctcgg ttcggttcag
     1621 cctctcccaa cccagcacgg catagacctg gcgcgaggcc gaggagaacg tttcgatcgt
     1681 gaagatcagg tcgtcctttg gccgttcgac ggtaatcaga gccgactccg gctctcgatc
     1741 cccggtcatt gcgcccgaca gcgcgaccga ctgcgagtcg gcgaagatca catttgtgac
     1801 ccggatattg gacgcgtcgc catccagctt gaacgtcgat tgcacgccag acgaccaccc
     1861 ctggccgtcg tagacgtcgt agcggcggag cgccaggtaa tgaccggagt cagactggac
     1921 cctggcaact acctcgttac tgaggtcgat gttgccgccg atctcgaagt tatcggggaa
     1981 gcttgcgtag ctgcctccgc cgggcccggt tttgccaatg accgactcga ccttgtcgcg
     2041 gagctgctcc catccctgct gggccgtctc ccggtatggc accgacatcg cccggtggat
     2101 gccgggattc accagcaatg cggccaccac ggcggccacg gcgacgggca gtgccgcaac
     2161 caggaagcgg gatgccagac ccggtccggt catcatgttc cgccgcgccc agcgcatcga
     2221 gttcgccgcg atcgcatgcc gggcagccaa tccgatcgcc gcgaagatga agagcgcgag
     2281 gggccagccg ccgttgcgat tgtcgactct gatcgatgcg agcaggatcg aggcgggcac
     2341 cgccacgcca aggccgaacc agccacgccg gtagatcacc cacgctgagc tgtaggcgag
     2401 gagccaggcc gtcacgccca gcacgacgag caactgcgcg tcggtcacgg agctggtatc
     2461 gtcgcggacc agcgactggc tgacgccggt gacaaggtcg acaaaggcag cgcccttgct
     2521 gcgaacgatc tcgaccgggc cggccgtctc gagcgtcacc agcagaacag cggccatggc
     2581 gccggtccag agcgcaatcg agtgcgcgac aagatcctgg gcgcgcgtct gggagagcag
     2641 atacccaagt acgagtccca tcagcgccag gggcccgagc acgccgatgc ggtcaccaag
     2701 ggcaacccgg ttcatcgcgc cgacgacgct caggaccagg acagcgtgca ggctgagcgc
     2761 caatccctcg gccacatatt caggcccggc aatcggctgt ttggatgcac tgccagtgag
     2821 ggtagccatc gcctaccgca cccatgctcc ggcgaatccg ttggatatct ggagcgcgct
     2881 cgttggatga tcgtcgcggc tcatccggta tccgacaaca tgctcgcgat gcagatcctc
     2941 gaggaaattg gtgatcgtca cgtcggggtt catatgggca tctacgaaat ggacttccga
     3001 cgagattctg cgacgccgca tcgttgccag aagcgcgacg aggttgggat cggcaaatga
     3061 ggtcacggtc agcagcacgc tgtccttgcg aaaccgcccg gtcagtgacg taagcaactc
     3121 gaccagcgtc gcatcgtccc gcccggtcac gaccgcgagg agctcgagga ttcgcacgta
     3181 ctggcggtcg ctgcgctcag gcggaagcac gatcggctgc tcggcattca tgatcaggcc
     3241 aacggatcgg ccgagcgcga gcgaccgccg gacgagcgac gccgccagca tgacccggta
     3301 ttcaaactcg ttgctcaacc agttcagcgg gtcgtccgac gcaaatcggg caccgtggga
     3361 aaagccatct gtcggaggcg tgtagaggtc cagcacgacc cagacatccg ctgtcgggtc
     3421 gttctcgaac tccttgacca tcaaccggcc agcacgagcg gtcgcggtcc aactgacgcg
     3481 gttgatcgaa tcgccttgca c
//
LOCUS       DATAOZ010000017         7045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5533111, whole genome shotgun sequence.
ACCESSION   DATAOZ010000017 DATAOZ010000000
VERSION     DATAOZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5533111"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VHR64_00365"
     CDS             complement(<1..402)
                     /locus_tag="VHR64_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3301307.1"
                     /translation="MITRRQLLAAVPAAALVTWRGPEIIRAQDAEVKVGFIYVGPIGD
                     MGWTYAHDQGRLELEKNLPNVKTTYQESVPENAADAERAIRQMAQDGNKVIFTTSFGY
                     MDPTINVAKDFPDTVFVHISGYKTADNVGTAF"
     gene            complement(693..1127)
                     /locus_tag="VHR64_00370"
     CDS             complement(693..1127)
                     /locus_tag="VHR64_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX3301308.1"
                     /translation="MPSRDFTVAVLVVRDQRILLHWHAKLGRWLPPGGHIEPDELPDE
                     AALREVMEETGVEAELISSPLNPINLPGQPRQLARPLGIQIADIGPDHQHIDLVYLAR
                     GLTEGNGAGAWISRDQLDGLELTEEVRVWCEIALDLAAEATN"
     gene            complement(1132..2055)
                     /locus_tag="VHR64_00375"
     CDS             complement(1132..2055)
                     /locus_tag="VHR64_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301309.1"
                     /translation="MALRREQLSPVDGFDNRIDWQGAMDRWNTRALRWVLGPGFLSCH
                     LTTFALSFLALLTWNLIADPADLRMIEPFRYWGVIVVFHIILLGGGMIGWKLLRMGET
                     EPRRFTIPAYQLPQLRANTTRTPHWQNAWTASAGRVNATARRWTGAASRQPEPPQPAP
                     APARDAETGWPEVPAMFRSAAVESNTTEAPPVDVGAATWPGSAPLSTTLANGGSSNPA
                     DEVVSVDHRAAEEAEEDPAKSWIDGFVESRSKDKDQRWSWVEAAAAAWLNKREVEGKT
                     EKALTAGESESESADATPPPDAADQSQISTD"
     gene            complement(2068..2523)
                     /locus_tag="VHR64_00380"
     CDS             complement(2068..2523)
                     /locus_tag="VHR64_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301310.1"
                     /translation="MPDLELIDRLVATYRILNHTVRLKPEAELAQGQTSPKAILRQLR
                     DDELRFSQELKARLSGQQVEIAGSADEAGMSSADSKDTTAEFIAQFGTARESTLSMLR
                     ILPDDQWDVTGDYPRSVRTDVISLIDRDRKTLDTIGQMLGEPLTEAATA"
     gene            2731..3111
                     /locus_tag="VHR64_00385"
     CDS             2731..3111
                     /locus_tag="VHR64_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301311.1"
                     /translation="MKPSNVLIPKDRVNAATPMRCPVCNGPLERVLIRDLGGVTADLV
                     WQLHAGHCPEHGWFQAEVVSQPPRDIFPVERPFGVARRMVIDDDEIFSFATVWNDMPA
                     EEKRRKDIDPFDRRYWKVRHANRG"
     gene            3123..4334
                     /locus_tag="VHR64_00390"
     CDS             3123..4334
                     /locus_tag="VHR64_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3301312.1"
                     /translation="MTERPQQRPADPTPAPAEGEWRRPGLTPLSLLLVLIAAFVLMKI
                     QLVVVLVLLAVLFATVIERPVMALHRRRVPRPLSILAVYVMIIGGIVLAGLLAAPLIS
                     NEAQRFRDEAPAQLRDLRAQWELGGNAALKGPGVNVLDRLLAEIDNPSSPSEDVTVGI
                     VTGVGGGLIGLLSVFVMAFYYLTEKALLRRIIINQVKPGARDRVERVWDQVEAQVGRW
                     LRGQLLLCFIIGSASFIGYGLMGVRFWPLLGLWAGITEIIPIIGPWLGGIPAVAIAAT
                     QSWDKAIMVIIFVVLLQFAENSILVPRVMRGAVGLTPLTVFVAILAGTQYLGVLGALL
                     AIPIAAAIQVIVSEYFKSRRETYRAVEAVSPGWRWMRGSTVSVTGPPEPATNGGTKTP
                     PAITPSPPSGR"
     gene            4344..4622
                     /locus_tag="VHR64_00395"
     CDS             4344..4622
                     /locus_tag="VHR64_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate o-methyltransferase 1"
                     /protein_id="HEX3301313.1"
                     /translation="MTDPTELTEERALELAWQAVERAGGTRAVYRNPKYAYTLQPTAD
                     MEIDGVKIELRHGEISSPAIVTVGNGWVFEIHDEDIELLIRPRVKRAE"
     gene            4965..5894
                     /locus_tag="VHR64_00400"
     CDS             4965..5894
                     /locus_tag="VHR64_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301314.1"
                     /translation="MFRVFSLLLMVGGLLAAQLAPAAAQNMDVSFSDVKLRELGYPEI
                     TITVGEDGVDAPSELAAGYYLITLQPTKEYSTYLDIMQPPAGLSTDEATRLALDAAAN
                     DLAQSGWGYLGGTNTFEVGVPVSFAIYLAPGEYAWAASYYQLSGEGDEIMKLVPLTVT
                     GEATPEAATAEATPAAGGPKADVRLEMTDDLVYMVSPDPLPTGPQIWEVANTGTKQSH
                     HLVMQRIPEGTTAEQILAEFGGMMAGTPPAQDSIAMQAVYVGYAALQSGGYTTYQEFD
                     LDPGTYAVICFIIDPETGMPHVMNGMVTVFTVE"
     gene            6035..6730
                     /locus_tag="VHR64_00405"
     CDS             6035..6730
                     /locus_tag="VHR64_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301315.1"
                     /translation="MSERPGIEHLLTERPETSDALRIQILATEHWNLLMTRSMTWNEM
                     FTRAGMYLTVVSAATVALALMAQATDFDDTFRLFALLVLPVVLFIGLVTHIRLGDARG
                     EDVWLVIGMNRLRHGYLEVAPELAPYFISSQYDDMAGVAQTYGFQGPPRPSRILASTP
                     VFVGVINAVVAGVLVGLVVEALGGSLTLSVAIGILAGVVIVVLMAGIVPHRAISKLDR
                     EYQPMFPHDSSEP"
     gene            complement(6740..>7045)
                     /locus_tag="VHR64_00410"
     CDS             complement(6740..>7045)
                     /locus_tag="VHR64_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3301316.1"
                     /translation="VELEWGVGSTDNDPAMTAIVRDCAEQVVGPENVTEGDLLMVSED
                     MSEFLSRVPGCYYLVGSMNESRGLTWGHHTSRFDIDEDALGIGIETMTRTVLTYLGR"
BASE COUNT         1155 a         2133 c         2442 g         1315 t
ORIGIN      
        1 gaaagcggtg ccgacgttgt cggccgtctt gtagccggag atgtggacga agaccgtgtc
       61 ggggaaatcc ttggcgacgt tgatggtcgg gtccatgtag ccgaaggagg tcgtgaagat
      121 gaccttgttg ccgtcctgcg ccatctgccg gatcgcccgc tcggcgtcgg cggcgttctc
      181 gggcacgctc tcctggtagg tcgtcttgac gtttggcagg ttcttctcga gctcgagccg
      241 gccctgatcg tgggcgtagg tccagcccat atcgccgatc ggtccgacgt agatgaagcc
      301 gaccttaacc tcggcatcct gcgcgcggat gatttccgga cctcgccacg taacgagtgc
      361 ggccgccggt actgccgcaa gcaactgacg cctggtgatc actgtgttct gccctttccc
      421 tgaacacgaa tcaaccgatt gttgatcgga acccgaatgg tgaatctccg gcgcgatgaa
      481 ctgcaggcgc caggcgggtg gtgcgtcgtt gcgtttccct ctatggaaaa ggatagccaa
      541 tcagtcccgg ccgcgcagca tgtgtcgcca tgacacgaat ttgtgtcacg ggcagcaatg
      601 ccgtgctcac aaactccgaa aaggatcgtc aacacgcgac gcgcgccgat cgggtagaca
      661 atccggaccg tgccagagag tcgaatctcg gcttagttgg tagcctcggc agcgagatcg
      721 agggcgatct cgcaccagac gcgcacctct tctgtcaact caaggccgtc gagctggtcg
      781 cggctgatcc aggcgccggc gccgttgccc tccgtcaggc cgcgggcgag gtagacgaga
      841 tcgatgtgct ggtgatccgg gccgatgtcg gcgatctgga tcccgagcgg tcgggcgagt
      901 tggcgcggct ggccggggag attgatcggg ttgagcggcg aagagatgag ctctgcctcg
      961 acgccggtct cctccattac ctcgcggagg gcggcttcgt ccggcagctc atccggctcg
     1021 atgtggccgc cgggtggcag ccagcggccg agcttggcgt gccagtgcaa gagaatccgc
     1081 tgatcgcgca cgacaagcac ggccacggtg aaatcgcgcg atggcacgaa ctcagtcggt
     1141 cgaaatctgg gactggtcgg cggcgtccgg cggtggagtg gcgtcggccg attcggactc
     1201 ggactcgccg gcggtaaggg ccttctcggt cttgccttcg acttcacgct tgttcagcca
     1261 ggccgcagcc gcagcctcga cccaggacca gcgctggtcc ttgtccttcg agcgggactc
     1321 gacgaagcca tcgatccagc tcttggcggg atcctcctcg gcttcctcgg cggcgcggtg
     1381 atcgaccgag acgacctcgt cggccggatt cgatgagccg ccgttggcga gggtggtcga
     1441 gagcggggct gagccgggcc aggtggccgc gccgacatcg accggcggag cctcggtggt
     1501 attcgactcg accgcagcgg atcggaacat tgccggaacc tcgggccagc cggtttcggc
     1561 atcgcgggcc ggtgccggcg cgggttgcgg tggctccggc tgccggctcg ccgcgccggt
     1621 ccagcgacga gcagtggcat tgacgcggcc ggcggaagcg gtccaggcgt tttgccagtg
     1681 cggcgtgcgg gtggtgttgg cccggagttg cggtaactgg taggccggga tcgtgaagcg
     1741 acgaggctcg gtctcgccca tgcggagcag cttccagccg atcatgccgc cgccgagcag
     1801 gatgatgtgg aacaccacga tcacgcccca gtagcggaac ggctcgatca tgcgcaggtc
     1861 ggccgggtcg gcgatcaggt tccaggtcag cagcgccagg aacgacaacg cgaaggttgt
     1921 caggtgacag gagaggaagc cgggaccgag gacccagcgc agggcgcgcg tgttccagcg
     1981 atccatcgca ccctgccaat cgattcgatt gtcaaacccg tcgaccgggg aaagctgctc
     2041 tcgccgcagg gccatcgtgg tgttccccta cgccgtcgcg gcttcggtca gcggctcgcc
     2101 gagcatctga ccgatggtgt cgagtgtctt gcggtcgcga tcgatcaggc tgatcacgtc
     2161 ggtgcggacc gagcgcggat agtcgccggt gacatcccac tggtcgtccg gaaggatccg
     2221 gagcatggag agggtggatt cccgggcggt gccgaactgg gcgatgaact cggcggtggt
     2281 gtccttgctg tcagccgagc tcatgccggc ctcgtcggca cttccggcga tctcgacctg
     2341 ctggccggag agtcgcgctt tcagctcttg cgagaagcga agctcgtcgt cccggagttg
     2401 ccggaggatg gctttgggcg aggtctggcc ctgggcgagc tccgcctcgg gtttcaggcg
     2461 caccgtatgg ttgaggatgc ggtacgtcgc gacgagacga tcgatcagtt ccaaatcggg
     2521 catcgggcgg ggtcctccat cttcagcgaa acgacctcag catagcattg cgggtgctga
     2581 gcgcttcgtg aacagggtac accaaaactg cgtaaatctg cgcacgccga tgggcggacc
     2641 ggtagtcgcg aatagtccta tcggatgcta gactcgacac ggaaaccgcc ggatcggcgg
     2701 cgcttggtca ttcctgcctg gagcaccttc gtgaagccat ccaatgtcct gattcccaag
     2761 gatcgcgtga atgccgccac gccgatgcga tgtcccgtct gcaacgggcc gctcgagcgc
     2821 gtgctgatcc gcgacctggg cggcgtgact gccgatctcg tctggcagtt gcatgccggg
     2881 cactgcccgg agcatggatg gttccaggca gaagtcgtct cccagccacc tcgggacatc
     2941 ttcccggtgg agcggccgtt cggggttgcc cggcggatgg tcatcgacga cgatgagatc
     3001 ttctcgttcg ccacggtttg gaatgacatg ccggccgaag agaagcgacg caaggatatc
     3061 gacccgttcg accggcggta ctggaaggta cgccacgcca atcggggata gcgcgcacat
     3121 tcgtgactga acgcccacaa cagcgccctg ccgacccaac acccgcgccg gcggaaggag
     3181 aatggcgacg tccgggattg acgccgctct cgctgctcct tgtcctgatc gcggccttcg
     3241 tgctgatgaa gattcagctc gtggtcgtgc tggtgctgct ggcggtcctg ttcgcgacgg
     3301 tcatcgagcg gccggtgatg gcgctccacc gccgccgcgt gccgcgaccg ctgagcatcc
     3361 tcgcggtcta cgtgatgatc atcggcggca tcgtgctggc cggattgctg gcggcgccgc
     3421 tgatctccaa tgaggcacag cggttccgag atgaggcgcc ggcgcaactc agggacctgc
     3481 gggcccagtg ggaactcggc ggcaatgcgg ccctcaaggg gccgggcgtc aacgtgctcg
     3541 accgcctgct ggccgagatc gataacccat cgagcccgtc cgaggacgtg accgtcggca
     3601 tcgtgaccgg tgtcggcggc ggactgatcg ggctactctc cgtcttcgtg atggccttct
     3661 actacctgac cgagaaggcg ctgctgcggc ggatcatcat caaccaggtc aaaccgggag
     3721 cgcgggaccg ggtcgagcgt gtctgggacc aggtcgaggc gcaggttggc cgctggctac
     3781 gcgggcagtt gctgctctgc ttcatcatcg gatcggcctc gttcatcggc tacggactga
     3841 tgggcgtccg cttctggccg ctgctcgggt tatgggccgg cattaccgag atcatcccga
     3901 tcatcggtcc atggctcggc gggatcccgg ccgtcgcgat cgccgcgacg cagagctggg
     3961 acaaggcgat catggtgatc atcttcgtgg tgctgctcca gttcgcggag aactcgatcc
     4021 tggtgccccg cgtgatgcgc ggcgcggtcg gtctgacgcc gttgacggtc tttgtggcga
     4081 tcctggcggg cacccagtac ctgggggtgc ttggcgcttt actggcgata cctatcgcgg
     4141 cggcgatcca ggtgatcgtg tccgagtact tcaagtcgcg gcgcgagaca taccgggcgg
     4201 tcgaggcggt ctcgccgggg tggcggtgga tgcgcggctc gaccgtttcg gtgactggtc
     4261 cacccgagcc ggcgacgaac ggcggcacca agaccccgcc cgcgatcacg ccgtcgccgc
     4321 cctccggcag gtaggtgggc ggggtgactg atccgaccga gctgaccgaa gagcgcgccc
     4381 tggagctggc ctggcaggcg gtcgagcggg ccggcgggac tcgcgcggtt taccgcaatc
     4441 cgaagtacgc ctacacgctc cagccgaccg cggacatgga aatcgacggg gtcaagatcg
     4501 agctgcggca tggggaaatc agctcgccgg cgatcgtgac ggtcggtaac ggctgggtgt
     4561 tcgagatcca tgatgaggat atcgagctgc tgatccggcc gcgggtgaag cgggcggagt
     4621 gattgggttt tgggttttcg gaacgagcgt acctctcgct ccgccgggaa ttgaaatccc
     4681 gggatcccta aacgtagcgc ggggtcacct caccccgcca agcgcatccc cacgcatggt
     4741 caatagaatc gaacacgtga cggggttggt cccggttacg gacgtgtcat catcgggtcc
     4801 gggcggggcg tggtaacccc gcgctacgtc agtcgtcccc tccgggtgcc agtcggtcgc
     4861 gagtgggtgc cgatttgacc atttgccggt accggctgct gtagactgcc gcggcgattg
     4921 tcgtggggca gttgtgcttg ggtttaccgg aaggaatgcg gttggtgttt cgcgttttca
     4981 gtctcttgtt gatggttggc gggttgctgg cggcgcaatt ggcgccagcg gcggcccaga
     5041 acatggatgt ctcgttcagc gacgtcaagc tgcgggagct cggctaccca gagatcacga
     5101 tcacggtcgg cgaggatggc gtggacgcgc cgtcggagct tgcggcgggg tattacctga
     5161 tcacgctcca gccgaccaag gaatactcga cgtatctgga catcatgcag ccgccggcgg
     5221 gactctcgac cgatgaggcg accaggttgg cgctggatgc cgcggccaac gacctggcgc
     5281 aatccggctg gggatacctg ggcgggacga acaccttcga ggtcggcgtg ccggtctcgt
     5341 tcgcgatcta cctcgcgccg ggcgagtatg cctgggcggc ctcgtactac cagttgtcgg
     5401 gcgaaggcga cgagatcatg aagctggtgc cgctgacggt gaccggcgag gccacccctg
     5461 aggcagcgac agccgaggcg acccccgcgg ccggcggtcc gaaggccgat gtgcggctgg
     5521 agatgaccga tgacctggtc tacatggtct cgcccgatcc gctgccgact ggtccgcaga
     5581 tctgggaagt ggccaacacg ggcaccaagc agtcacatca cctggtgatg cagcggatcc
     5641 cggagggcac gacggccgag cagatcctgg ccgagttcgg cgggatgatg gccggtacgc
     5701 cgccggcgca ggactcgatc gcgatgcaag cggtctacgt cggctatgcc gcgcttcagt
     5761 cgggcggcta tacgacgtac caggagttcg atctcgaccc ggggacgtac gcggtgatct
     5821 gcttcatcat cgacccggag accggaatgc cgcacgtgat gaacgggatg gtgacggtct
     5881 ttacggtgga gtagcgactg gttgatggtg attggtggtt ggttgatgga agagatatcc
     5941 aaccaccatc aaccatcatc cgacttggcc ctcacttgcc ggggatatga tggaggcgcg
     6001 tgctttctgg ctccaagcgg cgtgagggac tgtgatgagc gagcgtcccg ggattgagca
     6061 tctcctaacg gaacgaccgg aaacctccga tgcgctgcgc attcagattc tggcgactga
     6121 gcactggaat ctgctgatga cgcggagcat gacctggaac gagatgttca cccgggccgg
     6181 gatgtacttg acggtcgtct cggcggcgac ggtcgcgctg gccctgatgg cgcaggcgac
     6241 ggatttcgac gacacgtttc ggctcttcgc gttgctggtg ttgccagttg ttcttttcat
     6301 cgggctcgtg acccatatcc ggctgggcga cgcacgcggc gaggatgtct ggctcgtgat
     6361 cggcatgaat cgcctgcgcc acggctacct cgaggtcgcg ccggaactcg ctccctactt
     6421 catcagcagc cagtacgacg acatggccgg ggtcgcccag acgtacgggt tccagggacc
     6481 gccgcgtccg agccgaatac ttgccagcac accagtcttt gtcggcgtga tcaacgcggt
     6541 ggtggccggc gtgctggtcg gactcgttgt cgaagcgttg ggcggatcgc tcacgctgtc
     6601 ggttgcgatc ggaatcctgg ccggcgtagt gatcgtcgtg ctgatggccg gcattgtccc
     6661 gcatcgcgca atctcaaaac tggatcggga ataccagcct atgttcccgc atgactcgtc
     6721 ggagccctga ctgctcgcct tatcgaccta gataggtcag cacggtgcgg gtcatggttt
     6781 cgatgccgat gccgagggcg tcctcgtcga tgtcgaagcg gctggtgtgg tggccccagg
     6841 tcagcccgcg cgactcgttc atgctcccga ccaggtaata gcagccgggg acgcgcgaga
     6901 ggaactcgct catgtcctcg gagaccatca gcaggtcgcc ctcggtcacg ttctccgggc
     6961 cgaccacctg ctcggcgcag tcgcggacga tcgcggtcat ggccgggtcg ttgtcggtcg
     7021 agccgacgcc ccattcgagc tcgac
//
LOCUS       DATAOZ010000018         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3166575, whole genome shotgun sequence.
ACCESSION   DATAOZ010000018 DATAOZ010000000
VERSION     DATAOZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3166575"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..887)
                     /locus_tag="VHR64_00415"
     CDS             complement(114..887)
                     /locus_tag="VHR64_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301317.1"
                     /translation="MPDDGLCGFGPYWRVDVHATDFVTIGLLVLLEGLLSADNALVLA
                     VLVLGLPKPDQRKALRYGILGAFAFRFIATVFAAWMIQLSWVKLIGALYLLYLPVKHF
                     TGGDAEERRKPKPARAWLGLTAFWATVVRVELTDIVFAIDSILVAVAMSPKLWVIVTG
                     GLLGILAMRLLIGKLLNVVQRYPPLVDGAFVIIAWVGIKLLLEFLHQLEVVPFALPKW
                     LSLGLIVVIFGIAYFYARKRGPVLAEDQAGGLYDSGPEN"
     gene            980..1327
                     /locus_tag="VHR64_00420"
     CDS             980..1327
                     /locus_tag="VHR64_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3301318.1"
                     /translation="MRIDEDLFFISVAARLLGMHPQTLRKYERLGLVQPTRTIGSMRV
                     YSRDELERLKLIKRLVEDGGINLAGVQRLLSIAEVVQRIKPLARGESLTRGEARRLTA
                     EVNRLADLLGLDI"
     gene            1337..>1690
                     /locus_tag="VHR64_00425"
     CDS             1337..>1690
                     /locus_tag="VHR64_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013983884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX3301319.1"
                     /translation="MEFKDYYNVLGVNKTATDKELKQAYRKLARKYHPDVNPGDKTSE
                     SRFKEINEAYEVLGDPEKRKKYDELGSNWQAYERAGAYPGGGPPPGFEGVWNNPGGGS
                     YRTVSPEEMEELFGNE"
BASE COUNT          356 a          519 c          561 g          254 t
ORIGIN      
        1 cgcgatccgg aattcctgac ccagatccag tcgcgcgcga cgcgcgtcga actcagcgag
       61 ggcgggacgc agaccgtgtc gctgaagctg ttggataacc gttagcttct cgatcagttc
      121 tcgggtccgc tgtcgtacag cccgccggcc tgatcctccg cgagcacggg gccgcgcttg
      181 cgcgcgtaga aatacgcgat cccgaagatc acgacgatga gcccgagcga cagccacttg
      241 ggcagcgcga acggtacgac ttcgagctga tgcaggaact cgagcagcag cttgatgccg
      301 acccaggcaa tgatcacgaa cgcgccatcg accagcggcg gatagcgctg caccacgttc
      361 aacagcttcc cgatcagcag ccgcatcgcc agaatcccga gcaggccgcc ggtcacgatc
      421 acccacagct tcggcgacat cgccacggcc accaggatcg agtcgatcgc gaagacgatg
      481 tcggtcagtt cgacgcggac gacggtcgcc cagaaggccg tcagtcccag ccaggcccgg
      541 gctggtttcg gcttgcgtcg ctcctccgcg tcgccgccgg tgaagtgctt caccgggagg
      601 tacagcaggt agagcgcccc gatcagcttc acccagctca gctggatcat ccaggcggcg
      661 aacacggtgg cgatgaagcg gaaggcgaag gcgccgagga tgccgtagcg aagcgctttg
      721 cgctggtcgg gcttgggcag gccgaggacc agcacggcca gtaccagggc gttgtcggcg
      781 ctgaggagcc cttcgagcag aaccaggagg ccaatggtga cgaaatcggt cgcgtgcacg
      841 tccacacgcc agtatggccc gaaaccgcac aagccgtcat ccggcaaaga cttgagacca
      901 gcccagcctt gctgcggacc ttagcaggga cttgacacga tccgtatcaa gtcctacact
      961 cgatccgtcg caagttctta tgcgtatcga cgaggatcta ttcttcatca gtgtggcggc
     1021 gcggctgctc gggatgcatc cgcagacgct gcggaaatac gagcggctcg ggctggtgca
     1081 gccgacccgg acgatcggca gcatgcgggt ctactcgcgc gacgagctgg aacgcctgaa
     1141 gctgatcaaa cgtctggtcg aggatggcgg gatcaatctt gccggcgtgc agcggctgct
     1201 ctcgattgcg gaagtggtgc agcggatcaa accgctggcg cggggtgaat cgctgacgcg
     1261 cggagaagcg cggcggctga ccgcggaggt caaccggctg gcagatctgc tcgggctgga
     1321 tatctagcga ggtcggatgg aattcaagga ctactacaac gtcctgggcg tcaacaagac
     1381 ggccaccgac aaagagctga agcaggcgta ccgcaagctc gcccgcaagt atcacccgga
     1441 cgtgaatccg ggcgacaaga cgagcgagtc gcgtttcaag gagatcaacg aagcctacga
     1501 agtgctcggg gatccggaga agcgcaagaa gtacgacgag ctgggctcca actggcaggc
     1561 ctacgaacgg gccggcgcct acccgggcgg cggaccgccg ccgggattcg aaggcgtctg
     1621 gaacaacccg ggcggcgggt cgtatcgcac cgtctcgccg gaggagatgg aagaactgtt
     1681 cgggaacgaa
//
LOCUS       DATAOZ010000019         8673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1063105, whole genome shotgun sequence.
ACCESSION   DATAOZ010000019 DATAOZ010000000
VERSION     DATAOZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063105"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VHR64_00430"
     CDS             <1..328
                     /locus_tag="VHR64_00430"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX3301320.1"
                     /translation="NAFAPEHLCLLTHSPWNLVPYVKHAGGLFIGEDSPEALGDYTTG
                     PSHVMPTGGSARFFSPVHVNDFMKVINVAAANRAALQRLGPATISLARAEGLTAHARA
                     IERRLD"
     gene            complement(325..900)
                     /gene="ruvA"
                     /locus_tag="VHR64_00435"
     CDS             complement(325..900)
                     /gene="ruvA"
                     /locus_tag="VHR64_00435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX3301321.1"
                     /translation="MIAGLRGEIVAKTDDAILVDVHGVIYQVRTSTSTLAEAGAVGDP
                     VDLRTALIVREDAMTLYGFATAEELRFFEVMTGVSGIGPRLACAILSTFKPDALYAAV
                     QQGDVDLLSTVPGIGKKTASRVIVELSGKLPEAQFVHQDVLQDRDVLDALRAMGYSAS
                     EAQQALVRANITPGMTTEERLVAAFQQLGEG"
     gene            complement(897..1772)
                     /locus_tag="VHR64_00440"
     CDS             complement(897..1772)
                     /locus_tag="VHR64_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3301322.1"
                     /translation="MIELAGGSVYVVDAHVHIFPDEIGRDRAAWLLRDGWFHALYRNP
                     KARLATAEDLIESMDLAGVDHSIVCGFPWSDNAHCTFHNEYMADAARRFPERISWLAI
                     VAPGNHAGQTAEECLASGAVGIGELNADAQGFVWTETSRLAPITEAAQAFDRPIMVHA
                     SEPLGHDYPGKGRATPDQLLALAESFPELRIVAAHWGGGLPFFELMPEIRLMLGNLAY
                     DSAATTYLYEHTVFEHAMRICGADKVIFASDYPVLRQDRLIARMAGTEWPSIEVARKV
                     MGGNAARMYRLELTP"
     gene            complement(1790..2710)
                     /locus_tag="VHR64_00445"
     CDS             complement(1790..2710)
                     /locus_tag="VHR64_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX3301323.1"
                     /translation="MANAPAVETRLDIRISSATDVGGRTENEDAIAVRELQPVDLGDG
                     ELVDGLLMALADGMGGHQRGEVASQLAIDTVQDVFISDPAGDIVPLLKQAFRRANELI
                     YAAWKGETPAQLMGTTLVVAATRGKYATIASIGDSRAYLIRAGRLQQITRDHSLVAEQ
                     VAQGNMTPEEARESPHRNIITHALGHKPKLDSKMPNVFEITLLEDDRLLLLTDGFFDV
                     VPDEDLLRLTLETESEVIAQKLTSIAKERGTTDNVSAIVATVIPRPNLALATAQTSAL
                     SRRTGPPVVPMAAAILLILFLLAVIAYFVF"
     gene            complement(2789..3499)
                     /gene="rpe"
                     /locus_tag="VHR64_00450"
     CDS             complement(2789..3499)
                     /gene="rpe"
                     /locus_tag="VHR64_00450"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX3301324.1"
                     /translation="MTAGSIWIGPSILSSDLLKLGEQIAIAEHAGVDFIHFDVMDGRF
                     VPNITFGMPVARAVREATRLPLDVHLMIVEPEKYVREFVEAGADTVTIQVEAAVHAHR
                     TLADIHEMGATPGIALNPGTPLSAVTELIPFIGNLLIMTVDPGFGGQTFIPAMLDKVR
                     RARELIDDRNPNCRLEIDGGIKASNIGRVVAAGADTFVAGSSIFDGTDRIAENVTALR
                     RAASVLVESDRSDSPNLG"
     gene            3583..4440
                     /locus_tag="VHR64_00455"
     CDS             3583..4440
                     /locus_tag="VHR64_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301325.1"
                     /translation="MHFSPKSQIAIGLAINIPSWIVAWFGPAPFRYHTFFPLWLGFII
                     AVDGLTRLISGTSLLQRLGWRSVALFLVSIPIWWVFEGANDRLGNWVYILPRHYPWLE
                     YRAEASLAFSTVAPAIFVVSELVRTTVLTGRIQWIRLAPSRKGLLAISAGGAALFVAT
                     MLWPDVLFPFVWISLFLAVDPLVQVLGGRSISGAVARGRWDPVIVLFAGTLVCGFFWE
                     FWNFWSMPKWTYRIAYADWFRLFEMPVLGYGGYLPFALEVFAFVALADRLLGLRIMNA
                     IRFDRANVE"
     gene            4563..5318
                     /locus_tag="VHR64_00460"
     CDS             4563..5318
                     /locus_tag="VHR64_00460"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX3301326.1"
                     /translation="MVSSILDRFEESFERLVEGSVGRVFKTPIQPAEIGRKLERAMVT
                     NQVVSVDSTIVPNDYRVVLNPQDMIAFADLLPGLGGQMETWLSEVADLHGFRLIDRMR
                     VLIVGDESVPRRAIQVTAAIADKPGVNQRQQEELQRTELYRIVAKANGVQPLRFKITN
                     GPQAGAEFLLRKSVTTLGRSLENDIVLESSEVSRQHARIELQGDGMRIIDLNSTNGTR
                     VNGRSIRSQSVKAGDDVTFGNLSAKILGDGSTS"
     gene            5343..5798
                     /locus_tag="VHR64_00465"
     CDS             5343..5798
                     /locus_tag="VHR64_00465"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3301327.1"
                     /translation="MIADIALPVSYDWFLLGTRLLIAAILLVFLWRVMIVVGRDALMM
                     GSAGSQVSLALLDETDHPVRGFRLSRRKPMTIGRDTSNDIVLTDRSVSGHHAVIRMMQ
                     GEWVLADLDSRNGTYVNGAAIATETSISEQDVVQFGAVRLQLVSHESQI"
     gene            5861..7804
                     /locus_tag="VHR64_00470"
     CDS             5861..7804
                     /locus_tag="VHR64_00470"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX3301328.1"
                     /translation="MAEAIARLSESLSGDQRPADSAPASNRTAKSKFDVLLGLGNRLV
                     ILLTLLLLVGVSGLVIVRAMYSGKVMPSVYVADVPVGGMSKDEAMAVVQQRASSLVGE
                     TFVFDYDGRQWTTTLADLGVQPDVERSVNSAFAVGREQQARDRVGNTLSLATGSQVVP
                     LALKLDSSKVEAWVTTVTGDIDRKPLDAQITITNGKLQVVPDQDGIVVDKDRLMAVIS
                     ESIRTLAPYRGPLPIKLSAPSIRAADLDPAVTQITTALSSPIKIVYKEKQWMLQPADL
                     GQFIVQTPNAAGKGVTVSVDDSALGQWLYNLIGERINRDPVNAEIKWSEKQGKVVAVS
                     ESSKGITLLAGPLADSVIQSLLGNHNDVDVPVKGVKPEVDSDHLDALNITTKLGVGTS
                     SFYGSDENRATNIWVGTGHLNGTVVRPGETFSFNNAIGDITPEAGYVEASVVDGERIG
                     KDVGGGICQVSTTVFRAAFLAGFPIGEWWPHLYRLAFYEYDGWTPGLDASILQSGPRE
                     DWGDFTFHNTTNGYLLIEAYVDGQTDVVTIYGPETGWDVYVSEPWEGKPILGDDQPDV
                     ETVDPDLPPGTVQQSEYRQDGLEISYERIVKDADGNVIDDWIAYSRFAARGDVWKVSP
                     DMKGQSPASLNPHKVGSDSGDGT"
     gene            complement(7869..8147)
                     /locus_tag="VHR64_00475"
     CDS             complement(7869..8147)
                     /locus_tag="VHR64_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX3301329.1"
                     /translation="MADPESTILAIRVHPRSSKNRHAWTDGELHVWVTAPAVEGAANK
                     AVVKYLADVLDLPASRISFVSGETSRAKRLRVPVAEDIVAGKLSNGGA"
     gene            complement(8154..>8673)
                     /locus_tag="VHR64_00480"
     CDS             complement(8154..>8673)
                     /locus_tag="VHR64_00480"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX3301330.1"
                     /translation="EFYLMCGEPVPAADLYDGYPQIEDGIGITRHFLENLQTYLGRSK
                     AGSLAGVSATIACGTLIGPTMHSAIDDLNRHLGASVDVRVLENSFFGPEINISGLLTG
                     KDLIAGFANDPARTPVYISSRMISDRTQTLLDDLTVEQVQAELGRPVVPCLTLSDVAR
                     DLRHRHRTAQAA"
BASE COUNT         1632 a         2715 c         2687 g         1639 t
ORIGIN      
        1 aaacgccttc gcgccggagc acctctgtct gctgacgcat tcgccgtgga atctcgtgcc
       61 atatgtcaag cacgctggcg gcctcttcat cggcgaagac agcccggagg cgctaggcga
      121 ttacaccacg gggccgagcc acgtgatgcc gaccggaggc agcgcccgct tcttctcgcc
      181 agtccatgtc aacgacttca tgaaggtgat caacgtcgct gcggcaaacc gggcggcgct
      241 gcagcgactc ggaccggcca cgatttcgct cgcccgcgcc gaggggttga ccgcgcacgc
      301 cagggcgatc gagcgccggc tggattagcc ttcgccgagc tgctggaaag cggcaaccag
      361 gcgctcctcg gtcgtcattc cgggcgtgat gttggcccga accagtgcct gttgcgcctc
      421 ggaggccgag taccccatcg cccggagcgc atcgagcaca tcccgatcct gcaacacgtc
      481 ctggtggaca aactgcgctt cgggcagctt gccgctcagc tcgacgatca cgcgcgatgc
      541 agtcttcttg ccgattcccg gcacggtcga gagcaggtcg acatcgccct gctggaccgc
      601 cgcgtagagg gcatctggct tgaaggtcga gaggatcgcg caggccagac gcggcccaat
      661 ccccgagact cccgtcatga cctcaaagaa gcgcagctcc tcggcagttg cgaagccgta
      721 gagcgtcatc gcgtcctcgc gcacgatcag cgcagtccgg aggtcgaccg gatcgccgac
      781 agcgccggcc tcagccagcg tgctggtgct ggtgcgaacc tgataaatga cgccgtgaac
      841 gtcgaccagg atcgcgtcgt cggtcttggc gacgatctcg ccacgcagcc cggcgatcat
      901 ggagtcagct ccaggcggta catccgcgcc gcattgccac ccatcacctt cctcgcgacc
      961 tcgatcgacg gccactccgt gccggccatg cgcgcgatca gccgatcctg gcgaagcacc
     1021 ggatagtcgg aggcaaagat caccttgtct gctccgcaga tgcgcatcgc gtgctcgaac
     1081 accgtgtgct catacaggta cgttgtcgcc gcagaatcat aggcgagatt cccgagcatg
     1141 agccggattt cgggcatgag ctcgaagaac ggcagcccgc cgccccaatg tgccgcgacg
     1201 atccgaagct ccggaaacga ctcggcaagc gcgaggagct ggtctggtgt ggccctccct
     1261 tttccggggt agtcgtgtcc gaggggctcg ctggcgtgaa ccatgatcgg ccggtcaaat
     1321 gcctgcgctg cctctgtgat cggcgccagc ctgctcgttt ccgtccagac aaatccctgc
     1381 gcatcggcat tgagctcgcc gataccgacc gcaccggatg cgaggcactc ctcggccgtc
     1441 tgccccgcat gatttcccgg agctacgatg gcgagccacg agatccgctc cgggaatcta
     1501 cgcgccgcat cggccatgta ttcattgtga aacgtgcagt gagcgttgtc cgaccatgga
     1561 aacccgcaga cgatcgagtg gtcgacgccc gccagatcca tgctctcgat caggtcctcg
     1621 gccgttgcca gtcgcgcctt cgggttccgg tacagcgcgt ggaaccagcc atcccgcagt
     1681 agccacgcgg cacgatctcg accgatctcg tcgggaaaga tgtggacatg ggcatcgacg
     1741 acgtagaccg acccccctgc gagctcaatc acgtcatcga tcgcccggat cagaagacga
     1801 agtacgcgat aaccgcgagc aggaagagga tcagcaggat cgccgctgcc attggcacga
     1861 ccggcggacc ggtccggcgg ctcagggctg aggtctgggc tgtggcgagg gcgagattcg
     1921 gacgcggaat cacggtcgcg acgatcgctg agacgttgtc cgtcgtcccg cgctccttgg
     1981 cgatgctggt cagcttctgg gcgatcactt cggactcggt ttcgagcgtc aaccgcagca
     2041 ggtcttcgtc cggcacgacg tcgaagaaac catcggtcag gaggagcagt cggtcgtcct
     2101 cgagcaacgt gatctcgaaa acattcggca tcttcgagtc gagtttgggc ttgtgaccga
     2161 gcgcatgcgt gatgatgttg cggtgcgggc tttcgcgcgc ctcttcgggc gtcatgttgc
     2221 cctgcgcaac ctgctcggcg accagcgaat ggtcccgcgt gatctgttgc aggcggccgg
     2281 cccggatcag atatgcgcgc gaatcgccaa tgctcgcaat cgtggcgtac ttgccacgag
     2341 tcgccgcaac gaccagcgtc gtgcccatca actgagcggg ggtttcgcct ttccaggcgg
     2401 cataaatcaa ctcgttggcg cggcgaaagg cctgcttgag caatggaacg atgtcgcctg
     2461 cgggatcgga gatgaagacg tcctgcacgg tgtcgatcgc gagctgactc gccacctcgc
     2521 cgcgctgatg cccgcccatc ccatcggcca gcgccatcaa gaggccgtca accagctctc
     2581 catcgccgag gtcaaccggt tggagctcgc gtacggcgat cgcatcctcg ttttcggtgc
     2641 gtccgcccac atcggtggcg gatgagatgc gaatatcgag tcgagtctcg actgccggag
     2701 cgtttgccaa cgaagttacc acggttgaag ggcccttccc gtacctacat acggaggccg
     2761 atcatagcac gctgagccag tcgttccctc accccaggtt aggagaatcg gatcgatctg
     2821 attcgacgag tacggacgca gcccgccgca gcgcagtcac attttcggca atccggtcag
     2881 tcccgtcgaa gattgacgag ccagcgacaa aggtgtccgc gccggccgcg acgacccgac
     2941 cgatattcga cgccttgatc ccgccgtcaa tctcgaggcg gcaattcggg ttgcggtcat
     3001 cgatcagctc gcgcgcccgg cgaaccttgt cgagcatcgc gggaatgaac gtctgtccgc
     3061 cgaatccggg atcaacggtc atgatcagca gattcccgat gaacgggatt aactccgtca
     3121 cggccgagag cggtgtgccg ggattcaggg cgattcccgg cgttgctccc atttcgtgga
     3181 tgtcggccaa cgtccggtgg gcgtgaactg ccgcctcgac ctggatcgtg accgtgtcgg
     3241 cacctgcctc gacgaattcg cggacgtact tctccggctc gacgatcatg aggtgaacgt
     3301 cgagcggcaa tcgcgttgcc tcgcgcacgg cccgcgcgac gggcatgccg aatgtgatgt
     3361 tgggcacgaa tcgcccatcc atgacatcga agtggataaa atcgactccg gcatgttcag
     3421 cgatcgcgat ctgctcaccg agcttgagca ggtcggacga gagaatcgat gggccaatcc
     3481 agatcgatcc ggctgtcaca atcgcgtcct tcccgctccc gcaccatcgt gcccgacgac
     3541 catgcactat accgaaccca gcctgacaag acggatattc ccgtgcattt ctccccgaaa
     3601 tcccagatcg cgatcggact cgccatcaac attccgagct ggatcgtggc gtggttcgga
     3661 ccggcgccgt tccgatacca cacattcttc ccactctggc tcgggttcat cattgcggtc
     3721 gacggattga cccgactgat cagcggcacg tcgctcctcc agcgactcgg gtggcgatcg
     3781 gtcgcgcttt tcctggtgtc gatcccgatc tggtgggtct tcgagggagc caacgaccgg
     3841 ctggggaact gggtctacat cctcccgcgg cattacccgt ggctggagta ccgggctgag
     3901 gcgagtctgg ccttctcgac ggtcgcgccg gccatcttcg tcgtgtccga gctcgtccgc
     3961 acgaccgtct tgaccggccg aatccagtgg atcaggctgg cgccgagtcg aaaggggctg
     4021 ctcgcaatca gtgctggagg cgctgccctg ttcgtcgcca cgatgctttg gccggacgta
     4081 ctcttcccgt tcgtctggat cagtcttttc ctcgcagtcg acccgctcgt gcaggtgctc
     4141 ggaggtcgct cgatcagcgg cgcggtcgcc cgcggacggt gggatccggt catcgtgctc
     4201 tttgcgggaa cgctggtctg cggctttttc tgggagttct ggaacttctg gtcgatgccg
     4261 aaatggacgt accggattgc ctatgccgac tggtttcggc ttttcgagat gccggtgctc
     4321 ggatatggcg ggtatttgcc gttcgcactt gaagtctttg cgttcgtggc gctggccgac
     4381 cgactgcttg gtctcaggat catgaacgcg attcgatttg accgggccaa tgtagagtag
     4441 cgaatttggt caaaggtcct atgccgaacc acccgtccgt gattgagcgt taaagaagtc
     4501 gttcctataa tgcaggaacg gccgctttgg ccgtttcacc cgccgggagg cgtctgcgcg
     4561 gcatggtttc aagcatcctg gaccggtttg aagaatcgtt cgagcgactg gtcgagggat
     4621 cagtcggacg cgtcttcaag acgccgatcc agcccgccga aatcggccgc aaacttgagc
     4681 gagcaatggt cacgaaccag gttgtctcgg tcgactcgac catcgtgccc aacgactacc
     4741 gggtcgtgct caatccgcag gacatgatcg cctttgcgga cctgctgccc ggattgggtg
     4801 gtcagatgga gacgtggctg agcgaggtcg cggacttgca tggctttcgc ctgatcgacc
     4861 gcatgcgcgt tctgatcgtc ggcgacgagt ccgttccgcg ccgggcaatc caggtgacgg
     4921 ctgccattgc cgacaagccg ggcgtcaatc aacggcaaca agaggagctg cagcggaccg
     4981 agctctatcg gattgtggcc aaggcaaacg gcgtccagcc gctccggttc aagatcacca
     5041 acgggccgca ggccggcgcc gagttcctgc tgcgcaaatc ggtcaccacg ctcggccgat
     5101 cgctcgagaa cgacatcgtc ctcgagtcaa gcgaagtctc gcgacagcac gcgcggattg
     5161 aactccaggg cgatggcatg cgaataattg acctgaacag cacgaacgga acccgggtca
     5221 atggacgcag cattcgatcg caaagtgtta aagccggtga cgacgtcacc ttcggaaatc
     5281 tcagcgcgaa gatcctcggc gacggctcga cctcctagaa gcctcactcc gggcgacgcc
     5341 cgatgattgc cgatatcgcc ctcccggtct cctacgattg gttcctgctg ggcacccggc
     5401 tgttgatcgc cgcgatcctg ctcgttttcc tgtggcgggt catgatcgtg gtgggccgcg
     5461 atgcgttgat gatgggctcg gctggcagtc aggtttccct ggcgctcctc gatgagaccg
     5521 accaccctgt ccgcggcttc agattgtcgc ggcgcaagcc catgacgatt ggtcgggaca
     5581 cgagcaacga catcgtgttg actgaccgct ccgtttccgg acatcacgcc gtaatccgga
     5641 tgatgcaggg tgagtgggtc ctggccgatc tggatagccg caacgggacc tacgtaaacg
     5701 gcgccgcaat tgccacggaa acaagcattt ccgagcaaga cgtcgtacaa ttcggagcgg
     5761 ttcgccttca attggtgtct catgaatctc aaatttgaga tcgggcgtac caccttgcgg
     5821 aacgtacacg cagtacatca atggctgggg actgggaaac gtggctgaag caatagccag
     5881 gctttctgaa tcgctatctg gcgatcaacg accggcggac tccgctcccg catcaaatcg
     5941 caccgcaaaa tcgaagttcg acgtgctgct tgggctcggc aatcgcctgg tcatcttgct
     6001 gacgctgctc ctcctggtcg gcgtctccgg tctcgtcatc gtccgggcca tgtacagcgg
     6061 caaagtcatg ccgtccgttt acgtggcaga cgtccccgtt ggcgggatga gcaaagacga
     6121 ggctatggcc gtcgtccagc agcgagcctc atcactggtc ggcgagacat tcgtgttcga
     6181 ttacgacggt cgccagtgga cgacgacgct cgccgatctc ggcgtacagc cggatgtcga
     6241 gcgatctgtc aattccgcat ttgcagtggg acgcgagcag caggcgcgcg accgcgtcgg
     6301 gaatacactc agtcttgcta ccggcagcca ggtagtcccg ctcgccctga agctcgactc
     6361 gagcaaggtc gaagcctggg tcacgacggt gaccggtgac atcgatcgaa agccgctcga
     6421 tgcccagatc actattacga acggcaagct gcaggtcgtt cccgaccagg acgggatcgt
     6481 ggtcgacaag gaccgcttga tggcggttat ctctgagtcg atacggaccc tggcgccgta
     6541 tcgcggcccg ctcccgatca agctcagcgc tccatcgatc cgggccgccg atctcgatcc
     6601 cgcggtcacg cagatcacca ctgcactgag cagcccgatc aagatcgtct acaaggaaaa
     6661 acagtggatg ctgcaaccgg cagatttggg gcagttcatc gtccagacgc cgaacgcggc
     6721 cggtaaggga gtgacggtct cggttgacga ttcggcactc gggcagtggc tctacaacct
     6781 gatcggcgag cgcatcaatc gcgatccggt caacgccgaa atcaagtgga gcgagaagca
     6841 gggcaaggtc gtcgccgttt ccgagagctc gaagggcatc acgctgctgg ccggtccgct
     6901 ggccgactcg gtgatccaga gcctgctcgg caaccacaac gacgtcgatg tcccggtcaa
     6961 gggcgtcaag cccgaagtcg acagcgatca tctcgacgct ctcaacatca cgaccaaatt
     7021 gggcgtggga acatcgtcct tctacgggtc agatgagaac cgggcgacca acatctgggt
     7081 cggcacggga cacttgaacg gcacggtcgt gcggccaggt gagacgttct cgttcaacaa
     7141 cgcgatcggc gacatcacgc ccgaggccgg ctatgtcgag gcatcggtcg tggacggcga
     7201 gcgaatcggc aaggatgtcg gtggcggcat ctgccaggtc tcgaccacgg tcttccgtgc
     7261 ggcattcctg gccggattcc cgatcggcga gtggtggcct catctgtacc ggcttgcgtt
     7321 ctacgagtac gacggatgga ctccgggtct cgacgcatcg atcctgcaaa gcggacctcg
     7381 tgaggactgg ggcgacttca ccttccataa cacgacgaat ggctacctcc tgatcgaggc
     7441 ctatgtcgac ggccagaccg acgtcgtcac catttatggt ccagagacgg gctgggatgt
     7501 ctacgtcagt gagccatggg agggcaaacc aatccttggc gatgaccagc cggacgtcga
     7561 gacggtcgac ccggacctcc ctccgggcac ggtccagcaa tccgaatatc ggcaggatgg
     7621 tcttgagatc tcgtacgagc ggatcgtcaa ggacgccgat ggcaacgtga tcgacgactg
     7681 gatcgcctac agtcgcttcg ccgcccgggg tgatgtctgg aaagttagcc ccgacatgaa
     7741 ggggcaatca ccggcgagcc tcaacccaca caaggttggc agcgatagcg gcgatggcac
     7801 ctgagtcaaa tgttactgac tgagaagaca tgatgaagcg ggccgcccga tcgtggcggc
     7861 ccgttctcct atgcgccgcc gttcgataac ttgcccgcca cgatgtcttc ggcgaccggc
     7921 acacgtaacc gctttgcccg cgaagtctcc ccactgacga agctgatccg tgacgccggc
     7981 agatccagca catcggccag gtacttgacg acagccttgt tggccgcgcc ttcgaccgcc
     8041 ggggcggtca cccagacgtg caattcgccg tcggtccagg catgtcgatt cttgcttgag
     8101 cgcgggtgaa cccggatcgc cagaatggtt gattcgggat cggccatgcc cgatcaggcg
     8161 gcctgtgccg ttcggtggcg gtgtcggaga tcgcgagcaa cgtccgagag cgtcaaacac
     8221 gggacaactg gccgcccaag ctcggcctgc acctgctcga ccgtcaggtc atcgagcaat
     8281 gtctgcgtgc ggtcggagat catccgcgac gagatgtaga ccggcgtcct ggcgggatcg
     8341 ttggcgaacc cggcgatcag gtccttgccg gtcagcaggc cggagatgtt gatttccgga
     8401 ccgaagaatg agttctcgag aacgcggaca tcgacagacg cgccgaggtg ccgattcagg
     8461 tcgtcgatcg cgctgtgcat cgtcgggcca atcagcgtgc cgcaggcgat cgtcgcagat
     8521 accccggcga ggctgcccgc cttggaccgt ccgaggtacg tctgcaggtt ctcgaggaaa
     8581 tgccgggtga tcccaatgcc gtcttcgatc tgcggatagc cgtcgtaaag gtccgccgcc
     8641 ggcacaggct cgccgcacat caggtagaac tcg
//
LOCUS       DATAOZ010000020         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6325190, whole genome shotgun sequence.
ACCESSION   DATAOZ010000020 DATAOZ010000000
VERSION     DATAOZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6325190"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /gene="hisI"
                     /locus_tag="VHR64_00485"
     CDS             complement(<1..913)
                     /gene="hisI"
                     /locus_tag="VHR64_00485"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX3301331.1"
                     /translation="MTDAPLKFDEDGLIPVVIQDDSTGDVLMLAYMNLDALEQTRETG
                     RTHFWSRSRQTLWRKGETSGHEQIVKSIHVNCYENSLLVRVDQIGACCHTGFPTCFYR
                     ELTTDNQLIETESRRFDPESVYGDSGLAELETWLGAYRWLADHDLAGISGTSRHLRES
                     NVPVLSARVADELRELAGVVSGDHVHENQTSDALLEGSQVMYWIALTAIRAGLEDAEI
                     IDSIRAGTRSDVHEAPIDLPPKLRALADEWQRAFFRALSSLISRTISRIDLAAAATDT
                     SLSSMVARDLAELRTRPYLADYFEPGNS"
     gene            complement(910..1698)
                     /gene="hisF"
                     /locus_tag="VHR64_00490"
     CDS             complement(910..1698)
                     /gene="hisF"
                     /locus_tag="VHR64_00490"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX3301332.1"
                     /translation="MAELAPTTTLTKRIIPCLDVTDGRVVKGVEFVNLRDAGDPVELA
                     AFYNREGADELVFLDITASSDSRKTMIDVVSRTADQIFIPLTVGGGVRTIEDMRDLLA
                     AGTDKVSINTAAINDPSLITRGADHFGRQFIVVAIDARRQPDGTWAVYTHGGRRKTDL
                     DAVEWANRAAELGAGELLVTSMDRDGTRDGYDVALLEAISAAVNIPVIASGGAGTLAH
                     FSEALAPGRADAALAASLFHFGELRIADLKQYLAGQQIPVRLTT"
     gene            complement(1686..2399)
                     /gene="hisA"
                     /locus_tag="VHR64_00495"
     CDS             complement(1686..2399)
                     /gene="hisA"
                     /locus_tag="VHR64_00495"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX3301333.1"
                     /translation="MIIYPAIDIRDGKCVRLVEGDFNRETIFNEDPAEAARRWESEGA
                     THIHVVDLDGARNGEPTNFDAIERIRSAVSAQVQVGGGIRSLRSAQKLLDLGVNRIIL
                     GSAIVSDPEAAETIAGAYPGKVAAGLDARDGRLATNGWLSQSGMLATDAARRLIDSGL
                     TTMIYTDIRRDGTMSGPNLSALGEMIAIEGAEVIASGGIGSVDDVQAVAEAGAAGVII
                     GRALYDGRVRLKDALAWQS"
     gene            complement(2396..>3095)
                     /gene="hisH"
                     /locus_tag="VHR64_00500"
     CDS             complement(2396..>3095)
                     /gene="hisH"
                     /locus_tag="VHR64_00500"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX3301334.1"
                     /translation="PCRNPGQPLNDGNPVHEGGPRVIGVIDYGAGNIRSITRGLEAAG
                     AEVFVAQRGTDLAGADAIVLPGVGHAGQLMKSLQDRGFDRAVHDAVDADTPFLGICVG
                     LQIMFGDQEEGNTRGLGLLDGEVRHLPRELKTPHIGWSQVTAVTDSPFGPAGTSDHFY
                     FVHSYAASNPDANAVVATTDYGVTYPSVVIKDHLWGCQFHPEKSSTAGIAFLRTFVNY
                     VEAHLPARKAIASR"
BASE COUNT          548 a          961 c         1007 g          579 t
ORIGIN      
        1 aggagttacc gggctcgaag tagtcggcaa gatatggtcg ggttcgaagt tcggccaggt
       61 cgcgagcaac catcgaggag aggctggtgt ccgtcgctgc ggctgccaga tcgatccggg
      121 aaatggttct cgaaatcaga ctgctcagcg cgcggaagaa cgcacgctgc cactcatcgg
      181 ccagcgcgcg cagtttcggc ggtagatcaa tcggtgcttc gtgcacgtca gacctggttc
      241 cggcgcgaat cgagtcgatg atctcggcat cttcgagtcc ggcgcgaatt gctgtcagcg
      301 caatccagta catcacctgc gagccttcga gcagcgcgtc ggacgtctgg ttctcgtgca
      361 cgtggtcgcc gctgacgact cctgccaatt cgcgcagctc atccgcgact cgcgcgctta
      421 ggaccgggac attggactcg cgaaggtggc gggacgtgcc tgaaatcccg gccagatcgt
      481 ggtcggcgag ccaacggtaa gcgccgagcc atgtctcgag ttccgccagc ccgctatcgc
      541 cgtagaccga ttccgggtcg aagcgacgcg attcggtttc aatgagctgg ttgtcggtgg
      601 tcagctcgcg gtagaagcag gttggaaagc cggtgtggca acaggcgccg atctggtcga
      661 cgcgaacgag cagactgttc tcgtagcagt tgacgtggat cgacttgacg atctgctcgt
      721 ggcctgaggt ctcgcccttg cgccacagag tttggcggct acggctccag aagtgcgtcc
      781 tgcccgtttc gcgagtctgt tcaagcgcgt ccaggttcat gtaggcgagc atcaggacat
      841 cgcccgtcga gtcatcctgg atgacgaccg ggatcagacc atcctcatcg aatttcagtg
      901 gagcgtcggt catgtcgtca gtcgcaccgg aatctgttgg ccggcgaggt attgcttgag
      961 gtccgcgatc cgaagctcgc cgaaatggaa cagggaggcg gccagcgcgg catctgcccg
     1021 accgggcgcg agtgcctcgg aaaaatgcgc aagtgtcccg gcaccgcctg aagcgatgac
     1081 cgggatgttg accgccgcgc tgatcgcctc caggagcgcg acgtcgtagc cgtcgcgtgt
     1141 accatcgcgg tccatgctgg ttaccagcag ctcacctgcg cccagctcgg cggcgcgatt
     1201 cgcccactcg actgcgtcga ggtcggtctt gcggcgacca ccatgggtat atacggccca
     1261 cgtcccgtca ggctggcgac gggcgtcgat cgcaaccacg atgaactgcc gcccgaagtg
     1321 atctgccccg cgcgtgatca gcgatgggtc gttgatggcg gccgtgttga tggagacctt
     1381 gtcggtccca gccgcgagaa ggtcgcgcat gtcctcgatg gtgcgaactc cgccgccgac
     1441 tgtgagcggg atgaaaatct ggtcagccgt gcgcgagacg acgtcgatca tcgtcttccg
     1501 gctgtcgctg ctggccgtaa tgtcgaggaa gaccaactcg tcagcgcctt cccgattgta
     1561 gaaggcggcc agctcgaccg gatcgccggc atcgcgcagg ttcacaaact cgacgccctt
     1621 gacgacccga ccgtcggtca cgtcgaggca ggggatgatc cgcttcgtca gcgtggtagt
     1681 cggcgctagc tctgccatgc caatgcatcc ttgaggcgca cccgcccgtc gtaaagggcc
     1741 cggccgatga tcacgccggc cgcacccgcc tcggcaaccg cctgaacgtc gtcgaccgag
     1801 ccgatacccc ccgaggcgat gacctcggcg ccttcgatcg cgatcatctc gccaagagcg
     1861 gaaaggttcg gcccggacat cgtgccgtcg cgtcgaatat cggtgtaaat cattgtggtc
     1921 aagccagagt caatcaggcg ccgcgcggcg tcggttgcca gcatgcccga ttgcgagagc
     1981 caaccattgg tcgcgagccg gccgtcgcgt gcatcgagcc cggccgcgac cttgcctgga
     2041 tatgcgccgg cgatcgtctc ggctgcctcc ggatcggaga cgattgcgga gccgagaatg
     2101 atccggttca cgccgagatc gagcaacttc tgcgcgctcc tcagcgaccg gatgccgccg
     2161 ccgacctgga cctgcgcgga aaccgctgag cggatgcgct cgatcgcgtc gaagttggtc
     2221 ggctctccgt ttcgggcgcc gtcgaggtcg acgacatgaa tgtgcgtcgc gccttcggac
     2281 tcccagcgac gggctgcctc ggccgggtcc tcattgaaga tggtctcgcg gttgaagtca
     2341 ccttcgacga gccggacgca cttgccgtcg cgaatatcga tggccgggta gatgatcacc
     2401 tggatgcgat tgcctttcgg gccgggagat gggcctcgac gtaattgacg aaggtgcgca
     2461 ggaacgcgat tccggcggtg gaactcttct cggggtggaa ctggcagccc cagagatgat
     2521 ccttgataac cacgctcggg taggtcacgc cgtagtcggt tgtcgcgacc acggcattcg
     2581 cgtccggatt gctcgcggcg tagctatgga cgaagtagaa atgatcgctc gtgccggcgg
     2641 gaccaaacgg gctatcggtg actgccgtga cctggctcca gccgatgtgc ggcgtcttga
     2701 gctcgcgcgg gaggtgccgg acttcaccgt cgagcaaacc gagtcctcgc gtattgccct
     2761 cttcctggtc gccgaacatg atctgtagtc cgacgcagat gccgagaaac ggtgtatcag
     2821 catcgaccgc atcgtggacg gcgcgatcaa atccgcgatc ttgcaacgac ttcatcagtt
     2881 ggccggcatg gccgactccc ggcaacacga tggcgtcggc accggccaaa tcggtccccc
     2941 gctgcgcgac gaagacttca gccccggctg cttcgagtcc gcgggtgatc gatcggatat
     3001 tgccggcacc atagtcgatc acgccgatca cgcgaggacc cccttcgtgg acgggattgc
     3061 catcgttgag cggctgaccc gggttgcggc atgga
//
LOCUS       DATAOZ010000021         8067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4221909, whole genome shotgun sequence.
ACCESSION   DATAOZ010000021 DATAOZ010000000
VERSION     DATAOZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4221909"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VHR64_00505"
     CDS             <1..239
                     /locus_tag="VHR64_00505"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX3301335.1"
                     /translation="FPGELGLRIYEHVSAEAYELWKPQMTILINHYGLNPADPEARTF
                     LRQQMEDFFFGPGAQMPEGWVAPDAEVPARPRKK"
     gene            complement(352..1602)
                     /locus_tag="VHR64_00510"
     CDS             complement(352..1602)
                     /locus_tag="VHR64_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX3301336.1"
                     /translation="MTDRPAILLLATPSSYRLAAFIRAASSLDLDVIRGIDTPGAPPS
                     HVPDTLYLDFTFPRAAVSRLVGLVRTRPNTTVLAVDDAAVEIAASVNRRLRRSHNLPG
                     SDVASRDKAVMRQRFHDADLPTPERRLLELDLDPARISGALPYPVVVKPTRLNGSRGV
                     IRANDRAEFATAYERVRAMLTDEGFAPAEPAMLVESFIPGAEVAVEGLMTNGALTVLA
                     LFDKPDPLDGPYFEETIYLTPSRLPAGTQAAIAATTERMAEALGIRHGPVHAELRIND
                     DGVWPIEVAGRSIGGLCSTILEFGAGMPLEEIIIRHATGREIPSTERSGEAVGVMMIP
                     IPGEGVLRSVDGTADACDVPGITGIEITAPINQPIQPLPEGASYLGFIFARGKDPATV
                     ETAIRQAHARLKIHIAPIVRLAVV"
     gene            complement(1605..1832)
                     /locus_tag="VHR64_00515"
     CDS             complement(1605..1832)
                     /locus_tag="VHR64_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301337.1"
                     /translation="MDLVAADTRVFLFHSPDAAGDPGGAMDRVNSWLGKDRADSQYPN
                     LRVKDITVTPDGKGGVYTVVIVSLGKFSETL"
     gene            complement(2023..3288)
                     /locus_tag="VHR64_00520"
     CDS             complement(2023..3288)
                     /locus_tag="VHR64_00520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEX3301338.1"
                     /translation="MTASIDSTRSRLFSAIEDRTDELIATVAELVREPSVLGNEAGVQ
                     QVVARHLAESGMRVESYDLPDDTPQQPNGGHSGVPFAGRPNVHGYRTGTGGGRSLILN
                     GHVDVVSPEPVSAWTHDPWGAEIVGDRMYGRGAYDMKSGVGVNLFLSRLLNDLEIPLR
                     GDLTIHSVIEEECTGNGALAASLRDTADACLIPESTNFRLTTAHTGTIWFRVRIEGRS
                     AHAGWAWQGVNAIVKAVPVINALTKLNDELNLQVHPLWADQHHPINLNIGVIQGGDWP
                     STVPGACELRCRTSFFPGTTVEEMRAKIEGAVNAAAETDDWLREHRPVVIYDGFKTNG
                     VIVNQDEPMVELIRSTYASVTGQQLEDKVGPAVTDQRYYNFRGIPSVCFGARGDLAHG
                     ADEWLDLTSLPTVAKVMGSFILEWCGIAG"
     gene            3359..3429
                     /locus_tag="VHR64_00525"
     tRNA            3359..3429
                     /locus_tag="VHR64_00525"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3391..3393,aa:Cys,seq:gca)
     gene            3614..3886
                     /locus_tag="VHR64_00530"
     CDS             3614..3886
                     /locus_tag="VHR64_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301339.1"
                     /translation="MINPQVFVDEWWRKIARDTFFRTAATADLQCHSTGDETVGARHA
                     FIRMAEAIRVGDGHVFNNPEPLTTIVAQMLNTAEAGSAEPTSIPRS"
     gene            complement(3983..5002)
                     /locus_tag="VHR64_00535"
     CDS             complement(3983..5002)
                     /locus_tag="VHR64_00535"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3301340.1"
                     /translation="MNRPVNEQVVVITGASSGIGRETALEFGKCGASVVLAARNETAL
                     NEVASEIELAGGKAHAVVTDVAEWPQVEHLAQEAVTRFGRIDTWVNDAAVAEYATVEE
                     MSIEEIDRIIQVNLLGTIYGMKAALPHLKQEDSGTIINVSSVLGARAVPLQAAYTAAK
                     FGVRGFADSMRMELEHEGSNVHITTILPSSINTPFFDHARSKMGAEPKPIPPIYEPSA
                     VAEAILFAAENPRREIVIGAAGKMMTMMQRISPSFTDRYMLIGGQMFKQQKSDRPNGH
                     QDNLFTPSKGMGSSTGEFGGRMTQSRSLYTHFLGLHPNRGKAALAAGAGGVLGAVALK
                     RRTSN"
     gene            complement(5076..7652)
                     /locus_tag="VHR64_00540"
     CDS             complement(5076..7652)
                     /locus_tag="VHR64_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX3301341.1"
                     /translation="MSSTLTIVHSPHGIDHPYEPSFEERRPRDPAGGDMVALGFLTEP
                     GGAAESVRVEWARNGRKQTAIGARALSRGTDEDRWLVELGVVEAGDAVTYRIIGATVD
                     GTETATPEFTFTTRTWHTATQAIRLGGQDRYALIGSDGAPGPTITVVNDERGHRFAIR
                     QGDVSAYPDEAGTINIGELLDLGAGPDSAIVAMRWQEEAGRLTAIDLIGTLADSERLV
                     GFGERFDALNQRGRAFDSTVYEQYKNQGNRTYLPIPFFLSSEGYGLLVEGTTGVNFDL
                     GRTVPDRWRCVAQVPGSGRVAFQTFSGEPENIVRCLTAATGRPQPLPLWAYGLWMSSN
                     EWNTQARVEHEVAETVRHRIPATVLVIEAWSDEATFYIWNGAEYTATIGSGSLSLGDF
                     TFPADAPWPDPVGMTNSLHDQGIRLVLWQIPALKDVDGPHAQHEADVAYALDQEFVLQ
                     NEDGTPYRNPFFWFKNAHIPDFTSSDATAWWMSKRRYLLDELGIDGFKTDGAEHLAGR
                     GIRSADGRHGDELVNAYPMHYIAAYHDYARKHRNDDALTFSRAGYTGAGAYPAHWAGD
                     ENSTWEAYRRSIVAGLTAGLSGVIFWGWDIAGFSDTLPSAELYLRSTAMAAFCPIMQY
                     HSEYNPSGPSRDRTPWNIAATSGDERAISVFRHLARVRMNLLPYIAREAEFAASHGTP
                     LMRALLLDHPDDPIAWTIQDQYCFGHSLLIAPVVEEGATTRTLYLPAGTWIDLWSGQQ
                     QDGARWITVDAPWDRIPVFARAGAVIPLRLGHTGTLGDDTGNGLSLNDGLTWWLAVDP
                     RGETGGGDSELDVRFAVGSDGRLTVTVPPLETDVRLSAHGYRSLVLAKSNSAREFTLE
                     RG"
     gene            complement(7741..>8067)
                     /locus_tag="VHR64_00545"
     CDS             complement(7741..>8067)
                     /locus_tag="VHR64_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301342.1"
                     /translation="KQAEAAAKWVAFFTSDPEAAKVRVAASWELPALQDQALFQDYLA
                     LTPPDNREAVFESLDAIVTPPVIEKQQQMQDAVTAVLDQVKSGDMTPQEALDQAKTDI
                     EALIAS"
BASE COUNT         1377 a         2521 c         2704 g         1465 t
ORIGIN      
        1 ccttcccggg cgagctcggg ttacggatct acgaacacgt ctccgccgaa gcatacgagc
       61 tctggaaacc ccagatgacc atcctgatca accactacgg gctcaacccg gctgatcccg
      121 aggcccgcac cttcctccgc cagcagatgg aggatttttt cttcggcccc ggcgcccaga
      181 tgcccgaggg ctgggtcgcg ccagacgccg aggtgccagc gcggccaagg aagaagtgag
      241 ttaaggatag gcgcggagtc gggaggcgcg aaggcgcgga gttcatttct agaaacgaga
      301 aacaggaaac tccccgatcc cctaactctt aaccctcagc ccttaaccgg ttcagaccac
      361 cgctagccgc actatcgggg caatgtggat tttgagacgg gcgtgggctt ggcggatggc
      421 ggtttcgacg gtggccgggt ctttgcctcg ggcgaagatg aaaccgaggt agcttgcccc
      481 ttccggtaat ggctgaatgg gctggttgat cggcgcggtg atctctatcc ccgtaatacc
      541 gggcacgtcg cacgcatccg cagtgccgtc cacgctgcgc agtacgccct cgcctgggat
      601 cgggatcatc atcacgccga ccgcctctcc gctccgctcc gtcgacggaa tctcgcggcc
      661 ggtcgcgtgg cggatgatga tctcctcgag cggcatcccg gcgccgaact cgaggatggt
      721 cgagcagagg ccgccgatcg agcgtcccgc gacttcgatc ggccagacgc cgtcgtcgtt
      781 gatccgaagc tcggcatgta ctggaccatg ccggatgccg agcgcctcgg ccattcgttc
      841 cgtcgtcgcg gcaatggccg cctgggttcc ggccgggagc cgggacgggg tgagatagat
      901 cgtctcctcg aagtaggggc cgtcgagtgg gtccggtttg tcgaacaggg caagcaccgt
      961 cagggcgcca ttcgtcatca gcccctcgac cgcaacctcg gcgccgggaa tgaaggactc
     1021 gaccagcatc gccggctcgg cgggcgcgaa cccctcgtca gtcagcatcg cgcggacgcg
     1081 ttcgtaggca gtggcgaact cagcccggtc gtttgcccgg atcacgcccc ggctgccgtt
     1141 gaggcgcgtc ggtttgacga cgacggggta cggaagcgcg cccgagatgc gggccggatc
     1201 gaggtcgagc tcgaggagcc ggcgctcggg cgtcggcaga tcggcgtcgt gaaagcgctg
     1261 ccgcatgacg gctttgtccc ggctggcgac gtccgagccg ggcaggttat ggctccttcg
     1321 caggcgccgg ttgacggacg cggcgatctc gacagcggca tcgtcgaccg caaggacggt
     1381 ggtgttgggt cgggtgcgga cgagtccgac cagcctggag actgccgcgc gtggaaaggt
     1441 gaagtcgagg tagagggtat ccggcacgtg ggacggggga gcgccgggcg tatcgattcc
     1501 gcggatgacg tcgagatcga gcgagctggc cgcccgaatg aaggcggcca gccgatagct
     1561 actcggggtc gccagcaaca ggattgctgg tcgatcagtc atggttagag ggtttcggag
     1621 aacttgccga ggctgacgat gaccacggta tagacaccac ccttgccatc cggcgtgaca
     1681 gtgatgtcct tgacgcgcag gttcgggtat tgggagtcgg cccgatcctt gcccagccac
     1741 gaattgaccc ggtccatcgc tccgccgggg tcgcctgcgg cgtccggtga gtggaacaag
     1801 aagacgcggg tgtcggcagc aacgagatcc atcgatctca gtcctttcga acggtcggcc
     1861 acgtaccccg tggacgcgac cctctgatca ctaggcccgg ttcatccggg accggaaacg
     1921 aagtgtagtg gagagaaggc gcggagtcgg gaggcgcgaa ggcgcggagt tagtgatggg
     1981 tagatggtgg ttggttgatg ggtttccaac caccatccac cctcaaccgg cgatcccgca
     2041 ccattcgagg atgaagctgc ccatgacttt ggcgacggtt gggagggagg tcaggtcgag
     2101 ccattcgtcg gctccgtggg cgagatcgcc gcgtgcgccg aagcagaccg atgggatgcc
     2161 acggaagttg tagtagcgct gatcggtgac ggcaggaccg accttgtcct ccaactgctg
     2221 gccggtgact gaggcgtacg tcgatcggat caactccacc atcggctcgt cctggttgac
     2281 gatgacgccg tttgtcttga atccgtcgta gatcacgacc ggacgatgct cacggagcca
     2341 gtcgtccgtt tcagcggcgg cattgaccgc gccttcgatc ttcgcccgca tctcctcaac
     2401 ggtcgtgcca gggaagaagg aggtgcggca gcgcagctcg caggcgccag ggaccgtgct
     2461 cggccagtcg ccgccctgga tgacgccgat gttgagattg atcgggtggt gctggtctgc
     2521 ccagagcgga tggacctgga gattcagctc gtcgttgagc ttggtcagcg cgttgatgac
     2581 cggcacggcc ttgacgatcg cgttgacccc ctgccaggcc cagccggcgt gcgccgagcg
     2641 accctcgatc cggacccgga accagatcgt gcccgtgtgg gcggtcgtga ggcggaaatt
     2701 ggtcgattcg gggatcaggc aggcatcggc cgtgtcccgc aggctggcgg cgagcgcgcc
     2761 gtttccggtg cactcctcct cgatcacgct gtggatcgtc aggtcgcctc ggagtgggat
     2821 ctcgaggtcg ttgagcaggc gcgagaggaa gagattgacc ccgacgccgc tcttcatgtc
     2881 ataggcgccg cggccgtaca tgcggtcacc gacgatctcc gcgccccacg ggtcatgcgt
     2941 ccaggccgag accggttccg ggctgacgac atcgacatgg ccgttgagga tcagcgagcg
     3001 tccgccgccg gttcctgtgc ggtacccatg cacgtttgga cgaccggcga agggaacgcc
     3061 gctgtggcct ccgttcggct gctgcggggt gtcatctggg aggtcgtagc tctcaacgcg
     3121 catgcctgat tcggccaggt gacgggcgac gacttgctga acgccggcct cgttgccgag
     3181 caccgatggc tcgcggacga gttcagcgac agtcgcaatc agttcatcgg tgcggtcctc
     3241 gatagcggag aagagacggg agcgagtgga gtcgattgac gcggtcaatg aggtatccct
     3301 gcctgtgcgc gaaagcgaat tggacatggc gggatggtaa acttaaatgc tgcaacaggg
     3361 cgccatggcc aagtggtaag gcaagggtct gcaaaatcct gaccgccggt tcgaatccgg
     3421 ctggcgcctc cgagagcagc aactccccga caatctatcc aaacccggca cgacgtgcgc
     3481 cgaacgtctc cacggtagat tgcgtgatct ccgacttcgc gccgagtcag tacctttccg
     3541 ggtgctgggg agcaaccagt cgactgccaa cagaaagggc agcgtgattt cccaatctca
     3601 aactcatgcg ctcatgatca acccccaggt cttcgtcgat gagtggtggc gcaagatcgc
     3661 ccgggacacc ttctttcgga ctgccgcgac ggccgatctc cagtgccaca gcactggaga
     3721 cgagacggtt ggggcccgac acgccttcat ccgcatggcg gaggcgatcc gggtgggtga
     3781 tggtcatgta ttcaataatc cagaaccgct caccacgatc gtggcgcaga tgctgaacac
     3841 cgcggaggct gggtccgcgg aaccgacgag catcccccgg agctaatccg cccttcctct
     3901 cgtcgagcag atgtcgtccg gtactgccca gatcgcgcga gcgtgcgcgc aatccgggcg
     3961 ggtgatccgt cgtgccttgc cgtcagttcg aggtgcggcg cttgagcgca acggcgccga
     4021 ggacgccgcc ggcacccgcc gcgagcgctg ctttgcctcg attcgggtgc aagccgagga
     4081 agtgcgtgta gaggctgcgc gactgcgtca tccggccgcc gaactcgccg gtggacgacc
     4141 ccatgccttt cgaaggcgtg aagaggttgt cctggtgacc attcggccgg tctgacttct
     4201 gctgcttgaa catctgcccg ccgatcagca tgtagcggtc ggtaaatgac gggctgatgc
     4261 gctgcatcat cgtcatcatc ttgccggcgg cgccaatgac aatctcccgg cgcgggttct
     4321 cggcggcgaa caggatcgcc tcggcgaccg cgctcggctc gtagatcggc gggatcggct
     4381 tcggctcggc gcccattttg gagcgggcgt ggtcgaagaa cggcgtgttg attgatgacg
     4441 gcaggattgt cgtgatgtga acgttgctgc cttcgtgttc gagctccatc cgcatcgaat
     4501 cggcgaaccc gcgaacaccg aacttcgccg cggtataggc ggcctggagc ggaacggccc
     4561 gcgcaccgag cacggatgac acgttgatga tcgtgccgct gtcctcctgc ttgaggtgcg
     4621 ggagcgctgc cttcatgccg tagatcgtgc cgagcaggtt gacctggatg atccgatcga
     4681 tctcctcgat cgacatctct tcgaccgtgg cgtactcagc gacggcggca tcgttcaccc
     4741 acgtgtcaat ccgaccgaat cgggtgaccg cctcctgggc gaggtgctcg acctggggcc
     4801 attcggcgac atcggtcacg accgcatgcg ccttgccacc ggcgagctca atttcgctgg
     4861 cgacctcgtt cagggcggtc tcgttgcggg ccgcgaggac gaccgacgcg ccgcacttgc
     4921 cgaactcgag tgcggtctcc cggccgatgc cggacgatgc ccccgtaatg acaacgacct
     4981 gctcgttgac tgggcgattc atggggctct cctttccgtg tgcggtatcc agtctacgga
     5041 tcggttcgcc cagcccggtt gtctacgcca ccgagtcagc cccgttcgag cgtgaactcg
     5101 cgtgcggaat tcgatttcgc caagaccagg ctccggtatc cgtgagctga aagccggaca
     5161 tcggtttcga ggggcggaac tgtgactgtg agtcggccgt cgctgccaac ggcaaatcgc
     5221 acgtccagtt ccgagtcacc gccgccggtc tcacctcgtg ggtcgacggc cagccaccag
     5281 gtcaatccat cgttcaggct caacccgttc ccggtgtcgt cgccgagcgt accggtgtgg
     5341 ccgaggcgca gcgggattac cgcgccggcc ctggcgaata ccggaatccg gtcccacggc
     5401 gcatcgacgg tgatccagcg cgcgccgtcc tgttgctggc cggaccagag atcgatccag
     5461 gttccggcgg ggaggtagag cgtgcgcgtc gtggcgcctt cctcgacgac cggggcgatc
     5521 agcaacgaat gtccaaagca gtactgatcc tggatcgtcc aggcgatcgg gtcatccggg
     5581 tgatcgagca agagcgcgcg catcagcggc gttccgtggc tggccgcgaa ctcggcttcg
     5641 cgggcgatgt acgggaggag attcatgcgt acgcgggcga ggtggcggaa gacggagatc
     5701 gcccgctcgt cgccgctggt agccgcgatg ttccagggcg tgcggtcgcg cgaggggccg
     5761 ctcgggttgt attcgctgtg gtactgcatg atcgggcaaa aggcggccat cgcggtcgaa
     5821 cgaagataga gctcggctga gggtagtgtg tcgctgaagc cggcaatatc ccaaccccag
     5881 aagatgacac cgctgaggcc ggccgtcagg ccggcgacga tcgaccggcg gtaggcctcc
     5941 caggtcgagt tttcgtcgcc ggcccagtgg gcggggtagg cgcccgcgcc ggtatagccc
     6001 gcgcggctga aggtcagcgc gtcgtcgttg cggtgttttc tggcgtaatc gtgataggcc
     6061 gcgatgtagt gcatcgggta ggcattgacc agctcatcgc cgtgtcgacc gtccgccgat
     6121 cgaatcccgc gtccggcgag atgctccgcc ccatcggtct tgaaaccgtc gattcccagt
     6181 tcatcgagca agtagcgacg cttgctcatc caccacgcag tcgcgtcgga gctcgtgaaa
     6241 tccgggatat gcgcgttctt gaaccagaag aaggggttgc gatacggagt gccgtcctca
     6301 ttctgcagga cgaattcctg gtcgagcgca taggcaacgt cggcctcatg ctgcgcgtgc
     6361 ggcccgtcga cgtccttcag ggcagggatc tgccagagca cgagccggat gccctgatca
     6421 tggagcgaat tggtcatgcc gaccgggtcc ggccacgggg catcggccgg gaaggtgaag
     6481 tcgcccaggc tcagcgatcc gcttccgatt gtggcggtgt actcggcgcc gttccatata
     6541 tagaaggtcg cctcgtcact ccacgcctcg atcaccagga ccgtggccgg gatcctgtgg
     6601 cgaaccgtct cggcgacctc gtgctctacc cgggcctgcg tgttccattc gttggagctc
     6661 atccagagcc cgtacgccca aagcggaagc ggctggggtc gcccggtcgc ggccgtcagg
     6721 cagcgcacga tgttctcggg ctcgccggag aacgtctgaa acgcgactct ccccgaaccc
     6781 ggcacctgcg cgacgcagcg ccagcggtcg ggaacggtcc ggccgaggtc gaagttgacg
     6841 ccggtcgtgc cctcgacgag caagccgtag ccctcgctcg agaggaagaa cggaattggc
     6901 aggtaggtac ggttgccctg gttcttgtat tgctcgtaga cggtgctgtc gaacgcgcga
     6961 ccgcgctgat taagcgcgtc gaaccgttcc ccgaagccga ccaaccgctc ggagtccgcg
     7021 agcgtgccga tcagatcgat ggcagtcagg cgcccagcct cctcctgcca ccgcatcgca
     7081 acgatggccg aatctggtcc ggctccgagg tccagcaatt cgccgatgtt gatcgtgccc
     7141 gcctcatctg ggtatgcgga gacgtcaccc tgccttattg caaaccggtg cccgcgctca
     7201 tcgttcacca cggtgatggt cggaccgggc gcaccatctg atccgatcag ggcgtagcgg
     7261 tcctgaccac caaggcggat cgcctgcgtg gcggtgtgcc aggtgcgcgt cgtaaaggtg
     7321 aactcaggcg tcgccgtctc cgtgccgtca accgtggcgc cgatgatccg gtacgtgaca
     7381 gcatctccag cttcgaccac gccgagctcg accagccagc gatcctcgtc cgtaccgcgg
     7441 gatagtgcgc gggcgccgat cgcggtctgc ttccggccgt tgcgcgccca ttccacccgg
     7501 acactttccg ccgcgccacc gggttcagtc aggaacccga gtgcaaccat gtccccgccg
     7561 gcgggatcgc gcgggcggcg ctcttcgaac gaaggctcgt acgggtggtc gatcccatgc
     7621 ggtgaatgaa cgatagtcag cgtgctcgac acgtaattgc actccgtctg gttcgcggtc
     7681 cggatatgac gaccggcgtc gcagcagcca ttccgcgacg ccggtggggt aggggtagat
     7741 ctaggaggcg atcagcgcct cgatgtccgt cttggcctgg tcaagcgctt cctgcggcgt
     7801 catatcgccc gacttgacct gatcgaggac tgcggtcacc gcgtcctgca tctgctgctg
     7861 cttctcgatg accggcgggg tcacgatcgc atccagcgac tcgaagacgg cctcgcgatt
     7921 gtccggagga gtcagggcga ggtagtcctg gaagagcgcc tgatcctgca gcgccggcag
     7981 ctcccagctc gcggcgacgc ggaccttggc agcttcagga tcgctcgtga agaaggcgac
     8041 ccacttggcg gcggcttcgg cctgctt
//
LOCUS       DATAOZ010000022         8010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8169361, whole genome shotgun sequence.
ACCESSION   DATAOZ010000022 DATAOZ010000000
VERSION     DATAOZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8169361"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..882)
                     /locus_tag="VHR64_00550"
     CDS             complement(208..882)
                     /locus_tag="VHR64_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX3301343.1"
                     /translation="MQTEILYQPAYALCRVMLSAGETMRAESGAMVSMSGGVQIETKA
                     TGGIMKSLGRSLLGGESFFQNTFRAESGGEVTLSPELPGDIMSFDLAGHEIIVQSGSY
                     LASHTDITVETKWGGAKTFFGGEGLFMLKCSGTGTLIISSYGAIHRVSIPAGQKYVVD
                     TGHIVAFPSNVTYTVRKAAGWASTIFGGEGLVCEFQGPGDIFLQTRSPQAFLNWLIPQ
                     IPNRNN"
     gene            complement(1002..1655)
                     /locus_tag="VHR64_00555"
     CDS             complement(1002..1655)
                     /locus_tag="VHR64_00555"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX3301344.1"
                     /translation="MSGLLGGFEQWAQHVVEESGYPGLALLVALENIFPPIPSEVILP
                     LAGFLSAQGELWLPVTILAATVGSVAGALLLYGIGAWLGEERLRGVIRRYGHWLALGD
                     EDLSRANGWFEHHGTRAVLIGRVIPVVRSLISIPAGVNHMPLPSFILYTALGSGFWNS
                     VLIGAGWWLGDSWEQVSQYAQYLQYVLLAALIGGIGWFVYGRRHRQHDGAPSEESPR"
     gene            complement(1718..2665)
                     /locus_tag="VHR64_00560"
     CDS             complement(1718..2665)
                     /locus_tag="VHR64_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3301345.1"
                     /translation="MSTRNLAALVLLAAIWGSSYLFIRVAVDPLGPLMVAFARVGTAG
                     LGLAGYALLTGKRKDLAGLDRRFLMLGLGNAAVPYVLIAFAELHLTASMAGILNATTP
                     LFAAVVVAVWSHERITPRVSLGIVLGIAGVAVLVGWNPGPLTGIVLLAVVAMLLSSLS
                     YAVTTVYAKRSLRGVSSLRAAMGQQIGAAVLLGPFTAGAAAAGAGEGAPSLKVVLAVL
                     ALGLLCTSFAYLLYFHLIAAVGPVNTASTTFLIPVFSIIWSAIFLDEAIRPVMVLGLA
                     IILCSVTLVTGQQVPGIHRRLIRFRRGDMPGDQGPGTAT"
     gene            complement(2902..3375)
                     /locus_tag="VHR64_00565"
     CDS             complement(2902..3375)
                     /locus_tag="VHR64_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX3301346.1"
                     /translation="MGTAVGTAQARVENTAFWRMVPATKRAIIALGDSVEGAGLEPEL
                     LELVNLRCSQINGCAYCVQYHISNLQELGTAPAKINLVVAWREAGIFSAREEAAFAWA
                     ESLTLIAETHVPDADYDAARAVFSEQELASLTAAVATINVWNRFSVAYRFAPEID"
     gene            3440..3925
                     /locus_tag="VHR64_00570"
     CDS             3440..3925
                     /locus_tag="VHR64_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3301347.1"
                     /translation="MADDGKHPVDIPHAGEGKRGEQGYLGYLLRQASSAVRLGVDRAL
                     EDLGVTNPQFLVMTMIHAYPGISGAEVARLTMLTPQTISLIVANLERDGRLSRAASPT
                     HGRIQLMELTDEGIALLAHCRERARRLDARLSAGLTPDQEHFLRHWLVDIATRDLADD
                     T"
     gene            4095..4691
                     /locus_tag="VHR64_00575"
     CDS             4095..4691
                     /locus_tag="VHR64_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3301348.1"
                     /translation="MIDAEVGQQLRDLRRNRQMTVRQLAALTGLSRTSLTRIEAGTAN
                     PSVGTLKKVAGALGVSIGDLFINGAVDSRETESHVFSSSPAQSEVRVVRKDKRKTLSW
                     PGRPWKTYLLTPDLQRKLEVLLNDLEPGDTSDEFYTHEGEEFGFVMEGTYEITVGDNV
                     YVLEEGDSIYYPSHLPHKMRVVGDRPTKALWVITPPSF"
     gene            4824..6440
                     /locus_tag="VHR64_00580"
     CDS             4824..6440
                     /locus_tag="VHR64_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3301349.1"
                     /translation="MQDQSLSRLLAAVHADRLTRRRLLAAGAALAGTTLAPAALVGAQ
                     QATPAASPGATPVGKPGGTIKIAFEANPDDLNPFTMSSLVSALVVEQVYDTLFIFDEN
                     LASQPNLCTSYETPDDKTYLFHLAQNATFSDGTPLTSADVKFSLESYKDPNIGARAWA
                     QTIDSVETVDDVTVKVNLSQPYAPLIGYLSWQYNPIISKAFYEANNGDISQKVMGSGP
                     FLLQEFVPDQVIKFAKNPNYWQDGLPYLDAMEWTILPDDQARVAALRGNDVQNADFLD
                     HQAVESFENDPNWKIYEVSTLTHATTYINCSSGPLADARVRQALSYAIDRNEFLQGAA
                     LGYGQVTGYIPASDKTWSIPVSDLPTYQTNIDKAKELLAEAGYADGFDVKLRVSSLYI
                     LDTANAQILQQQLKPIGVNVEIEQLEWGNLLDAWVNSDFEMLNILLLGLPDPDGYCWG
                     RYHSTSPTNYNKISDSELDGLMDKARAETDLEARKSLYADIQKKLDTLVPNLFYYVYN
                     VWLIWDPSVQGITPLPNSSAPYMKRIWIEQ"
     gene            6500..7462
                     /locus_tag="VHR64_00585"
     CDS             6500..7462
                     /locus_tag="VHR64_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301350.1"
                     /translation="MATYLFRRLLMMIPTLLGVSIIVFLFIRLIPGDIVQVLSGAQGD
                     MTPERRELLRHNLGLDRPLVIQYLDWLSGVLRGDLGESLKTKRPINDDLLSRLPVTVE
                     LTIFSALIGLAIGIPAGVIAALKRGTRTELAAQGAGLVGLSVPDFWLGTLFLLVASRY
                     FGWYPGARYVSIFNAPLDNLAIFVLPSLAVGIGLSASLMRITRSSLLDVMGSGYIVTA
                     RAKGLRPRAVLFGHALKNALVPIITIIGLQLGYLLGGVVIVETVFNLPGVGRFVVDAI
                     SARDYPIVQASVLLITFIVLMVSLAVDVLYAYIDPRIKYSRTGS"
     gene            7459..>8010
                     /locus_tag="VHR64_00590"
     CDS             7459..>8010
                     /locus_tag="VHR64_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301351.1"
                     /translation="MTDVALKTAIVAPVEPRPVRGRFRQFARQFARNRAGMIGLAIIA
                     VIVLIALFAPIVAPFDPYEMNRIERLQGPTRTHPMGTDDLGRDLFSRIVYGARISMTV
                     GLVSVLIATILGTPAGIIAGYFGRWIDSVIMRVADVIFAFPAILLALAITAFLGPSLR
                     NVTISLGIVYAPGFARIVRGPVLA"
BASE COUNT         1514 a         2818 c         2277 g         1401 t
ORIGIN      
        1 ggacggcacc atcatcatcg accccgctcc ggtccgcgac ccactgaagc gcctccccct
       61 cggcggcgaa gagatcaagg tagccccggg ccagtaatca gtggtcagtg gtcagtggaa
      121 agctaaagga tcaggggtca agttcatctg acccctgatc cctgtttcct tcaactgacg
      181 actgaagtct gacgtctgcc gactcgctca gttgttccga ttcggaatct gcgggatcag
      241 ccagttcagg aacgcctgcg ggctgcgcgt ctgcaggaag atgtcgccag gaccctggaa
      301 ctcgcagacc agaccctcgc cgccgaagat tgtcgaagcc cagcccgccg ccttgcgcac
      361 ggtgtatgtc acgttcgacg ggaacgcgac gatgtgcccc gtatcgacca catatttctg
      421 cccggccggg atcgagacgc ggtggatcgc gccgtagctg ctgatgatca acgtgcccgt
      481 gccggagcac ttgagcatga acaaaccctc gccgccgaag aaggtcttcg cgccgcccca
      541 cttcgtctca accgtgatgt cggtgtggct ggcaaggtag ctgcccgact ggacgatgat
      601 ctcgtgcccc gccaggtcga acgacatgat gtcgcccggc agctccggcg aaagggttac
      661 ttcgccgccg ctttcggccc ggaacgtgtt ctggaagaac gactcgccac cgagcagaga
      721 ccgcccgagc gacttcatga tgccgccggt cgccttggtc tcgatctgca cgcccccgct
      781 catcgagacc atagcgccgg actccgcccg catcgtctcg cctgcgctca acatcacccg
      841 gcacaacgcg tacgccggct ggtacaaaat ttctgtctgc atggtctgcc cgatttctcc
      901 ccgatgtgtc caaaaaccga tccagcgaat gctaccgcct cgcgaccgca ccgtgtctga
      961 taacgccgga attggcagcc gggttccccg cgcggccagg attagcgagg cgactcttcg
     1021 ctcggcgctc catcgtgctg ccgatgtctc cgcccgtaga caaaccagcc gatcccgccg
     1081 atcaatgcgg ccagcagcac gtactggagg tactgcgcat actggctgac ctgctcccag
     1141 ctatccccca gccaccaacc cgcgccaatc aatacgctgt tccagaatcc gctcccgagc
     1201 gccgtgtaaa ggatgaagga cggcaacggc atgtggttca ccccggccgg gatcgagatc
     1261 aggctgcgca caaccgggat gacccggccg atcaggaccg ccctggtacc gtggtgctcg
     1321 aaccaaccat tcgcgcgact gaggtcctca tccccgagcg cgagccagtg cccatatctc
     1381 cggataactc cgcgcagccg ctcctcgccg agccacgcgc cgatgccgta cagcagcaac
     1441 gccccggcga ccgatcccac ggtcgcggca agtatcgtca cgggtaacca gagttcgccc
     1501 tgcgcggaga gaaatccggc cagcggcaga atcacctcgg acggaatcgg cggaaagatg
     1561 ttctcgagcg cgaccagcag cgccaatccg ggatagcccg attcctcgac gacgtgctgc
     1621 gcccactgct cgaacccacc cagaagaccg ctcattggcc cagcctactc cgcacgcata
     1681 cgcctggcca atgtcaggaa tacagcatga tccaggatca ggtcgccgtg cccggcccct
     1741 gatcgcccgg catgtctccc cgccggaacc ggatgagccg ccggtgtata cccggcacct
     1801 gctgcccggt caccagcgtc acgctacaga ggatgatcgc cagccccagc accatcaccg
     1861 gccggatcgc ctcgtcgagg aagatcgcgc tccagatgat gctgaagacc gggatcagga
     1921 acgtcgtgct cgcggtattg accggaccga ccgccgcgat caggtggaaa taaagcaggt
     1981 aggcgaacga ggtgcagagc aggcccagcg ccagcaccgc cagcacgacc ttgagcgacg
     2041 gcgccccttc acccgctccg gccgcagccg cgccagccgt gaacggcccc agcagcaccg
     2101 ctgcgccaat ctgctggccc atcgccgccc gcaacgacga cactccgcgc agcgaccgct
     2161 tcgcgtagac ggtcgtcacc gcatatgaga gcgatgacag caacatcgcc accacggcca
     2221 gcagcacgat cccggtcagc ggccccggat tccagcccac cagcaccgct acgcccgcga
     2281 tcccgagcac gatccccagg ctgacccgcg gcgtgatccg ctcatggctc cagaccgcca
     2341 ccacgaccgc ggcgaagagg ggagtggtgg cgttcaggat ccccgccatc gaggccgtca
     2401 ggtgcagctc cgcgaaggca atcagcacgt acggcaccgc cgcattgccc agaccgagca
     2461 tcaggaagcg gcgatccagc ccggccagat ccttccgctt cccggtcagg agcgcatacc
     2521 cggccaaacc cagaccagcc gtgccgaccc gcgcaaaggc caccatcagc ggacccagag
     2581 ggtcaaccgc aacccgaata aagaggtaag aggaacccca gatcgccgcc agcaagacca
     2641 gggcagccag gttacgggta ctcaccaggt agatatctcc gcgccaatgg gatgatcgct
     2701 cagaaaacgt agcgcggggt taccatgccc cgcttgatgc atccgtacgc acggccgata
     2761 gaaccgagcg acttaccggg attggttccc gcgatagacc ggccgcaatc gggtccggac
     2821 ggggcgtggt cctctctccg gctctctctg caaggagaga gagcatcccc gcgctccgtc
     2881 aacggacggt tcgtgcgccc gctaatcgat ctcgggcgcg aaccggtacg ccaccgagaa
     2941 gcggttccag acattgatcg tcgccaccgc agcggtcagg ctggccaact cctgctcgga
     3001 gaagaccgcc cgggccgcat cataatcggc gtccggcaca tgcgtctcgg cgatcaatgt
     3061 caggctctcc gcccaggcga aggccgcctc ctcgcgcgcg gaaaagatgc ccgcttcccg
     3121 ccacgcgacg acgaggttga tcttcgccgg cgccgtcccc agttcctgca agttgctgat
     3181 gtggtattgg acgcaatagg cgcagccgtt gatctgcgag cagcgcaggt tgactagctc
     3241 gagcagttcc ggctccagtc cggcgccctc gaccgaatcg ccgagcgcga tgatcgcccg
     3301 tttcgtcgct ggcaccatcc gccagaacgc cgtgttctcg acccgcgcct gtgccgtccc
     3361 gactgcagtt cccacgactc acacctcgct tcgtgatata tcagtgctct tatattcgag
     3421 ctctgatata atactgcgta tggcagacga cggcaagcac ccggtcgaca tcccgcacgc
     3481 gggagaaggc aaacgcggcg agcagggcta cctcggctat ctgctccgcc aggccagcag
     3541 cgccgtccgc ctcggagtcg accgcgcgct cgaggatctc ggcgtcacca atccccaatt
     3601 cctggtcatg accatgatcc acgcctaccc gggcatctcc ggggcggagg tcgcccggct
     3661 gaccatgctc accccgcaga cgatcagcct gatcgtcgcg aatctcgaac gggacggccg
     3721 cctgagccgc gccgccagcc cgacccacgg ccgcatccag ctgatggaac tcaccgacga
     3781 aggcatcgcg ctcctcgccc attgccgcga gcgcgcccgc cgcctcgacg cccgcctcag
     3841 tgccggcctc acgcccgacc aggagcactt cctccgccac tggctcgtcg acatcgccac
     3901 ccgcgacctc gcggatgaca cctgaccacc tcgccagcct ggtttgttcg gtgtatcggg
     3961 gagtcccgcc ggtcccgttc caggtcaccc ggcggatcgg aacacgagac gccttacgaa
     4021 ccggggaaga atgtccatta tagtgggcgt ctcagtgagc gcttcttcat tccagaattc
     4081 cggagttcaa gatcatgata gacgctgaag tgggtcagca gcttcgggac ttgcgccgca
     4141 accgccagat gacggtgcgg cagcttgctg ccctcacggg actctcgcgg acctccctga
     4201 cacgaattga ggccggtacc gctaacccgt cggtcggcac cctgaagaaa gtggcgggcg
     4261 cgctcggcgt atcaatcgga gatttgttca tcaacggcgc cgtagattct cgtgagacag
     4321 aatcacatgt attctcaagc tcacctgctc agtcagaggt gcgcgtggtc cgcaaggaca
     4381 agcgcaaaac gctctcctgg cccggacgcc cctggaagac ctacctcctc actcccgacc
     4441 tgcagcggaa gctggaagtg ctgctcaacg atctcgagcc cggcgacacg tcggacgagt
     4501 tctacacgca cgagggtgag gaattcgggt tcgtcatgga ggggacctac gagatcacgg
     4561 tcggggacaa tgtctatgtg ctcgaagagg gcgacagcat ctactatccg agccaccttc
     4621 ctcacaagat gcgcgtcgtc ggcgaccgcc caacgaaggc gctctgggtc attacgccgc
     4681 cgtcgttctg attacgcacg cgcaggaatg caccatggaa tggtcggtgt gttcaatatt
     4741 cttgacacct gttgcaaccc gatggtaggt tgccggaaat cgacacaagt gacgtcgccg
     4801 accatttcat gagggagctt accgtgcagg atcagtccct ttctcgtctc cttgctgcag
     4861 tgcacgctga tcgcctgacc cggcggcgcc ttcttgccgc gggagccgcg ctcgccggca
     4921 cgacgctcgc gccggccgca cttgtcggcg cgcagcaggc cactccggcc gccagccccg
     4981 gcgccacccc cgtcggcaag ccgggcggca cgatcaagat cgccttcgaa gccaacccgg
     5041 atgatctcaa tccgttcacc atgtcgtcgc tggtctcggc gctggtggtg gagcaggtct
     5101 acgacacgct cttcatcttc gacgagaacc tggcctccca gcccaacctt tgcacgagct
     5161 acgagactcc ggacgacaag acctaccttt tccacctcgc ccagaacgcc accttctcag
     5221 atggaacccc gctcacctcg gccgacgtca agttctcgct cgagagctac aaggacccga
     5281 acatcggcgc ccgcgcctgg gcccagacaa tcgactccgt cgagacagtc gacgacgtca
     5341 cagtcaaggt caacctctcg cagccctacg cgccgctgat cggctacctc tcctggcagt
     5401 acaacccgat catctcgaag gccttctacg aggccaacaa cggcgatatc agccagaagg
     5461 tgatgggttc gggaccgttc ctgctccagg agttcgtccc cgaccaggtg atcaagttcg
     5521 ccaagaaccc caactactgg caggatgggc tcccgtacct cgacgcgatg gaatggacga
     5581 tcctgcctga cgaccaagcc cgcgtcgcgg cgctgcgcgg caatgacgtc cagaacgccg
     5641 acttcctcga ccatcaggcg gtcgaatcgt tcgagaacga cccaaactgg aagatctacg
     5701 aggtctcgac cctcacccat gccacgacct atatcaactg ctcctccggc ccgctcgccg
     5761 acgcccgggt ccgccaggcg ctcagctacg cgatcgaccg gaacgagttc ctccagggcg
     5821 cagcgctcgg ctacggccag gtgactggat acattcccgc ctccgataag acctggtcga
     5881 tcccggtcag cgatctaccc acataccaga ccaacatcga caaggccaag gagctcctgg
     5941 ccgaagcggg atacgccgac ggcttcgacg tcaagctccg ggtctcatcg ctctatatcc
     6001 tcgacacggc caacgcccag atcctgcaac agcagctcaa gccgattggc gtgaacgtcg
     6061 agatcgagca gctcgagtgg ggcaacctgc tcgacgcctg ggtcaacagc gacttcgaga
     6121 tgctgaacat cctgctcctg ggcctgccag atccggacgg ctactgctgg ggccggtacc
     6181 actcgacctc gccgaccaac tacaacaaga tcagcgactc cgagcttgac ggactcatgg
     6241 acaaggcccg cgctgagacg gacctcgagg cccgaaagag cctttatgcg gacatccaga
     6301 agaagctgga cacgctggtt cctaatctct tctactacgt ctacaacgtc tggctgattt
     6361 gggatcccag cgtgcagggt atcaccccgc tcccgaactc cagcgcgccg tatatgaagc
     6421 gaatctggat cgagcagtag accgttgaag cgtttggggg atcggcgcgc ggccgatccc
     6481 ctttcatctc cgggacgccg tggccaccta tctttttcgt cgtctgctca tgatgattcc
     6541 gaccctgctt ggcgtctcga tcatcgtttt cctgttcatc cggctcatcc cgggtgacat
     6601 cgtccaggtg ctttccggcg cgcagggaga catgacgccg gagcgccgcg aactgctccg
     6661 gcacaatctc gggctcgatc ggccgctggt catccagtac ctcgattggc tctccggagt
     6721 gcttcgcggc gacctcggcg agtcgctcaa gaccaaacgg ccgatcaacg acgatctgct
     6781 cagtcgtctg ccggtgacag tcgagttgac cattttttcc gccctgatcg ggctggcgat
     6841 cgggattccc gccggcgtca ttgcggcgct caagcggggc acgcgtacgg agttggccgc
     6901 tcaaggcgct ggcctcgtcg gcctctccgt gcccgatttc tggcttggaa ccctcttcct
     6961 gctcgtagcc tcccgctact tcggttggta tccaggcgcg cgctacgtca gcatcttcaa
     7021 tgccccgctc gacaacctcg ccatcttcgt actgccgtcg ctggcggtcg ggatcggcct
     7081 ctcggcctcg ctgatgcgga tcacccgatc ctccctgctc gacgtcatgg ggagtggcta
     7141 catcgtcacg gcgcgcgcca agggactacg gccgcgggca gtgctcttcg gccacgcgct
     7201 caagaacgcc ctcgttccga tcatcacgat catcggcctc caactcggct acctgctggg
     7261 tggcgtcgta attgtcgaga ctgtcttcaa cctgccaggc gtggggcgat tcgtagtcga
     7321 cgcgatctcg gcgcgagatt acccaatcgt gcaggcctcg gtcttactca tcaccttcat
     7381 cgtgctcatg gtcagcctcg cggtggatgt gctctacgcc tacatcgacc cgcggatcaa
     7441 gtacagccgg acaggatcat gacagacgtc gcgctcaaga ccgccatagt cgccccggtc
     7501 gagccacgtc cggtccgggg tcggttccgc cagttcgcgc ggcagttcgc ccggaaccgc
     7561 gcaggcatga tcggcctggc catcatcgcc gtgatcgtgc tgatcgcgct tttcgctccg
     7621 atcgtcgcgc cgttcgaccc atacgagatg aaccggatcg agcgccttca gggaccaacc
     7681 cgcacacacc caatgggtac cgatgacctc ggccgcgatc tcttcagtcg tatcgtctac
     7741 ggtgcgcgca tttccatgac cgtcggtctc gtctctgtgc tgatcgcgac gatcctcggc
     7801 acgccagccg gcatcattgc cggctacttt ggccggtgga tcgactcggt aatcatgcgc
     7861 gttgccgatg tcatcttcgc cttcccggct attctgcttg ccctcgcgat caccgccttc
     7921 ctcggcccca gcctccgtaa cgtgaccatc tcgctcggca tcgtttacgc gcccggcttt
     7981 gcccggatcg tgcgtggacc ggtcctggcc
//
LOCUS       DATAOZ010000023         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3961888, whole genome shotgun sequence.
ACCESSION   DATAOZ010000023 DATAOZ010000000
VERSION     DATAOZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3961888"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..826)
                     /gene="istB"
                     /locus_tag="VHR64_00595"
     CDS             complement(98..826)
                     /gene="istB"
                     /locus_tag="VHR64_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX3301352.1"
                     /translation="MERTQLFDLMGELKLFGMKAAYDEIMATAVKRQHEPQRIVGDLL
                     NAEISEKQARSIRYQLATAKLPLAKDLDDFAFEGTPINQTLVTDLAGGGFIQQQRNVV
                     LIGGTGTGKTHLAIAIVRSCIRSGARGRFYNVVDLVNRLETETRNGRQGRLADYLTRL
                     DFIVLDELGYLPFAQSGGQLLFHLISRLYERTSIIVTTNLAFGEWPSVFGDAKMTTAL
                     LDRLTHHCDIVETGNESWRFKNRA"
     gene            complement(835..2191)
                     /gene="istA"
                     /locus_tag="VHR64_00600"
                     /pseudo
     CDS             complement(835..2191)
                     /gene="istA"
                     /locus_tag="VHR64_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611638.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          368 a          691 c          737 g          395 t
ORIGIN      
        1 agggaccccc ctggtgacgc gctccagcgc cctcctggtc atcgcggagc cgtaggcgca
       61 gcgatgacca ggagggcgag gcagcttctg atgaggctca ggcgcggttc ttgaagcgcc
      121 agctttcgtt gccggtctcg acgatatcgc agtgatgagt cagccgatcg agcagtgcgg
      181 ttgtcatctt ggcgtcgccg aacacgctcg gccattcgcc gaaggcgagg ttggtggtga
      241 cgatgatcga ggtgcgctcg tagagccggc tgatcaggtg gaacaggagc tgtccgccgg
      301 attgggcgaa gggcagatag ccaagttcgt cgaggacgat gaagtcgagg cgggtcaggt
      361 aatcggcgag acgtccctgc cgcccattgc gggtctcggt ctcgagccgg ttgacgagat
      421 cgaccacgtt gtagaaccga ccacgggctc cggatcggat gcagcttctg acgatggcga
      481 tggccagatg ggtcttgccg gtgccggtcc ctccgatcag gacgacgttg cgctgctgct
      541 ggatgaagcc gccgccggcg agatcggtga cgagggtctg gttgatcggc gtgccttcga
      601 acgcgaagtc atcgaggtcc ttggcgagcg gcagcttggc ggtcgccagc tggtaacgga
      661 tcgagcgggc ctgcttctcg ctgatctcgg cattgagcag gtcaccgacg atgcgctgcg
      721 gctcgtgctg gcgtttgacg gcggtggcca tgatctcgtc gtaggcggcc ttcatgccga
      781 agagcttgag ttcgcccatg aggtcgaaga gctgcgtgcg ttccatcaga gggttctccg
      841 gaggttgtcg tagcgggcac agtcggcgat cggggcatgg cgcagtgtca gcgcgtccgg
      901 cgtcatgatc gtcaccggcg ggtctggatc gcgctggcgg gccagaatgt tgagaaccac
      961 gtcggcggag tggacgccgt ggacgatggc ctcggcacag gccgcctcga ccgcgggcag
     1021 cccgtcgatg agcactgcag cgaggatgtc gaccatctga cgatcgccgt catcggtgcc
     1081 ggcgagcttg cgtcgcacgc gctccatcgc agccggcagc acccaatcct tgaagggtgc
     1141 accattgcgc aaagccccgg gcttgcgggc gagcaccggc acgtaatgcc aagggtcgta
     1201 gatggtctcg ccgcgaccga acgagcgggc atgctcggcc acgatccgcc cgtcctggcg
     1261 gatcaggatg cggtccgcat aggcatggac ctcgaccgga cgtccgatcg cgctcgccgt
     1321 caccgagtat cggttgttgt cgaagcgaac caggcaggtc ttggagaccg acgccggcac
     1381 cgcatggaag ccgtcgaagc gccccgcata gggaacgagc ttcggccgct ccgcctcgaa
     1441 cacctcccag atcgtctgct cggtcagttc cggatggcga tgcgccttgg cgtaggcgat
     1501 gcaccgatcg gtcagccagg cattgagttc gtcgtaactc ttgaaccgca gccgtggcgt
     1561 gaagaagcgc tcgcgcacca gcccgacctg gttctcgacc tgccccttct cccagccgga
     1621 tgccggcgtg caggccgtcg gttcgaccag atagtggctg cacatttgca ggaagcggcg
     1681 attgtagaga cggtccttgc cgatgaagat cgtctccacc gccgtcttca tgttgtcgta
     1741 gatgccgcgt ccgcaggtgc ctttgaacag cgcgaacgcc cggtcatggg cgtcgaacac
     1801 catctcctgc gtctcgcgcg gataggcccg cacgaacagc atccggctgt ggcagagccg
     1861 gacatgggcg accttcacca tcaccgtcgt gccgctgagc agcacgactt cgtggctcca
     1921 gtcgaactgg taggcctccc ccggcgcaaa gctcagcggc acataggccg ccgccaccga
     1981 ctgcccgcgc tccttggccc agcgccgggc atagcgtcga accgcatcgt agccaccgtc
     2041 ataaccgcgc ccgcgcaggt cctcgaagat ccggatcagg gtcagctgct cgcgtgcggc
     2101 cttggctgca ttgttcgcca gtaacgtctc gagttccgcc gtccatcggc caagcttcgg
     2161 ccgcggctgc acctcacgct catattcgaa c
//
LOCUS       DATAOZ010000024         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3436513, whole genome shotgun sequence.
ACCESSION   DATAOZ010000024 DATAOZ010000000
VERSION     DATAOZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3436513"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VHR64_00605"
     CDS             complement(<1..380)
                     /locus_tag="VHR64_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX3301353.1"
                     /translation="MSISDDWTYIRSVEYLINQWQFHILPVAAATQITQLFWGGAFGL
                     IFGVTPGVLRVSTIAIVLLSALALRDMFRDLKVSNQRAALGTALYLFNPIMFAISYSF
                     MSDPHFVAWMTLSAWLYLKAEMHDR"
     gene            707..1546
                     /locus_tag="VHR64_00610"
     CDS             707..1546
                     /locus_tag="VHR64_00610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3301354.1"
                     /translation="MKSEYYGEYYEHENSHWWFRWRFDMITQIVETLPKSEHFRLLDA
                     GCGTGQMTKLLEGYGDAVGLEIAPEAIAFARRRGVKNIVQGSIADPPFAAGSFDLVLS
                     LDVIEHVDNDVHILSSLFDILKPGGHLIVTVPAFEALWSEHDEINQHKRRYRVPQLRQ
                     MLIDADFDVSRVTYCNSALFLPVLAMRKGKTWWRRFRGHSIEDHPISDLAFYPKPVNE
                     ALYRILTAETKVMQHWDMPVGVSILAVAQRPLEAGADTAPANISIDSLIAEPDQQKVL
                     TAQ"
     gene            1554..2399
                     /locus_tag="VHR64_00615"
     CDS             1554..2399
                     /locus_tag="VHR64_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301355.1"
                     /translation="MNHLSVGRTIDVQPKPRTHRDGRALLAIIVISVLVIAGLWGISR
                     SAPSAKQLDSVSLVTTGVSGEQGCANFADFWMRDSGLHVPAEAIEGMSNCRLSMDGTW
                     FVPANAADPRLPADDHLTAQEREQVAVLSTQLADDIQQLEQILPRSLMETLKVNYTDE
                     NRPVFGHTKKGRQDLGAKWARYVQISQAYLLEPEHNVLADYVGWAMGRRLDAVSAFED
                     ACFADPANQFLWRACKGLRAEFAVNNIPLYWDLSDPVMIQEYLIDRVRSGQPLPAPTP
                     AAGKN"
     gene            2417..>2813
                     /locus_tag="VHR64_00620"
     CDS             2417..>2813
                     /locus_tag="VHR64_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX3301356.1"
                     /translation="MTDRVLIANRGEIAVRIVRACRDLGLATVGVYGPGDEQALHVRM
                     ADEAYRIESDRPIPYLDIGALTAIACRAKARLIHPGYGFLAENAIFAEACEKAGLVFV
                     GPPPDAIATMGDKVAARAAAVAAGVPILPG"
BASE COUNT          552 a          825 c          904 g          532 t
ORIGIN      
        1 cgatcgtgca tctcggcctt gaggtagagc caggcggaga gcgtcatcca ggcgacgaag
       61 tgcggatcgc tcatgaagga gtaactgatc gcgaacatga tcgggttgaa gagatagagc
      121 gcggtgccca gcgcggcacg ctgattgctg accttgaggt cgcggaacat gtcgcgcagt
      181 gccagggcgc tcaggagcac gatcgcaatc gtcgagaccc gtagcacccc gggtgtgacg
      241 ccgaagatga gtccaaaggc gccgccccag aagagttgcg tgatctgtgt cgcggcagca
      301 acgggcaaga tgtggaactg ccactgattg atcaggtact cgaccgaccg gatgtaggtc
      361 cagtcgtcgc tgatggacac cggcgccaat gtcggcagga acagggccgc caggaggtaa
      421 acggcaaaga ccgcagcggt gaacggatcc tgcgcgatga cgcggataag gcgatccagc
      481 agtgtcgtct cggcatatgt ttcgggcgtt tgagcgcgag ttgtatctgg cgatgttgcg
      541 gtaattggtt ggtctccacg cgaaccctga tggtgtcgcg tttttcgagc gttcggttac
      601 gaagtattat gatcgccggg ttcgggataa acggcgatgc cgccatggac cggtagcgat
      661 ctgctctcta tctacggcac acggcggaca ggagtcgtat caacggatga aatcggagta
      721 ctacggggaa tactacgaac acgagaattc ccactggtgg ttccggtggc ggttcgacat
      781 gatcacccag atcgtggaaa ccctgcccaa atccgagcat ttccgcctgc ttgatgccgg
      841 gtgcggcacg ggccagatga ccaagctgct cgagggatac ggcgacgccg tcggtctcga
      901 gatcgcgccg gaagcgatcg cgtttgcgcg gcggcgcggc gtcaagaata tcgtccaggg
      961 atcgattgct gatccaccct tcgcggccgg ctcgttcgat ctcgtgttgt cgctggacgt
     1021 catcgaacat gtcgacaacg acgtgcacat cctctccagc ctgttcgata tcctcaagcc
     1081 gggcggccac ctgatcgtga ccgtgcccgc gttcgaggca ctctggtcgg agcacgacga
     1141 gatcaatcaa cacaagcgac ggtaccgggt acctcaactg cggcagatgc tgatcgatgc
     1201 cgatttcgat gtcagccggg tcacctattg caacagcgcg ctcttcttgc ccgtgctggc
     1261 catgcgcaag ggtaagacct ggtggcgcag gttccggggc cactcgatcg aagaccatcc
     1321 gatatcggac ctcgcctttt acccgaagcc ggtcaacgag gcgctctacc ggatccttac
     1381 cgccgagacg aaggtgatgc agcattggga catgccggtt ggcgtctcga tcctggcggt
     1441 ggcgcagcgc ccgctcgagg ccggagccga taccgcgccg gcaaatatat cgatcgactc
     1501 gctgatcgcc gagccagatc aacagaaggt gctcactgcg caataattgc gccgtgaatc
     1561 acctatcagt tggccgcaca atcgacgtcc aaccaaaacc acgcacccac cgggatggtc
     1621 gggcactgct cgcgatcatc gtcatctcgg tgctggtgat tgccggactg tggggaattt
     1681 ctcgatcggc gccttcggcg aagcaactcg attcggtctc gctggttaca accggcgtct
     1741 ccggagagca gggttgcgcg aacttcgccg acttctggat gcgcgactcc ggacttcacg
     1801 ttccagcgga ggcgatcgag ggaatgtcca actgtcgcct gagcatggac ggcacctggt
     1861 ttgtcccggc caatgccgcc gatccgcgat tgcccgccga tgatcacctg actgcacagg
     1921 agcgagagca agtcgccgtc ctgagcaccc aacttgcaga tgatatccag cagctcgagc
     1981 agatcctgcc gcgttcgctt atggagacgc tcaaggtcaa ttacacggac gagaatcggc
     2041 cggtctttgg tcacacgaag aagggacgcc aggatctcgg cgccaagtgg gcccgctacg
     2101 tgcagatttc gcaggcatac ctgctggagc cggagcacaa cgtgctggcg gactatgttg
     2161 gttgggcgat ggggcgccgc ctggacgccg tcagcgcgtt cgaggatgct tgctttgccg
     2221 atcccgccaa ccagttcctc tggcgggcgt gcaaggggct gcgcgcggag ttcgccgtca
     2281 acaatatccc gctgtattgg gatctctcgg atccggtcat gattcaggaa tatctgatcg
     2341 accgggtccg atccggacaa ccattgcccg cgccaactcc cgcggccgga aagaattgaa
     2401 ggaagtctga ggcaccatga ctgatcgggt attgatcgcg aaccgaggcg agattgcggt
     2461 ccgaatcgtg cgggcgtgcc gggatctggg gctcgcgaca gtcggggtct acggtccggg
     2521 tgacgagcag gcgcttcatg tccgtatggc cgacgaggcg tatcggatcg aatcggatcg
     2581 gccgattcca tacctcgata tcggcgcctt gaccgcgatc gcctgccgcg ccaaagcccg
     2641 gcttatccat cccggctatg gattcctggc cgaaaatgcg atcttcgccg aagcgtgcga
     2701 gaaggcgggc ctcgtcttcg tcggaccgcc gcccgatgcg atcgcaacga tgggcgataa
     2761 ggtcgcggcc cgggcggcgg cggtggcggc cggcgtgccg atcctgccgg gga
//
LOCUS       DATAOZ010000025         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7118719, whole genome shotgun sequence.
ACCESSION   DATAOZ010000025 DATAOZ010000000
VERSION     DATAOZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7118719"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..1736)
                     /gene="hydA"
                     /locus_tag="VHR64_00625"
     CDS             complement(339..1736)
                     /gene="hydA"
                     /locus_tag="VHR64_00625"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX3301357.1"
                     /translation="MDTLIKNGTVVTAAGISKADVAVEGGKIALIGADLKAAGARIID
                     ASECYVIPGGVDVHTHLDTPGFDTVTADDFESGTRGAACGGTTTIVDFCQQAHGQSLA
                     DALRVWHEKAAGKASIDYGFHIIVRDMNDAVEAELARLPDEGITSFKLFMAYKGMLGV
                     DDLVLIRALEQARKAGALVMVHAENGDAAYFLQRKYVAEGKTAPKYHAPTRPPRVEAE
                     ATARAIALAELVGTSIYIVHLTCRESLEEVVRGRSRGVDVLAETCTHYLYVTKEDLDR
                     DGFEGAKYVFTPPARTREDHEILWHALAHRTLELVSSDHASWNYAGDKELGRDDFIKI
                     PNGAPGIEERLTMVYQGVDQGRLRLPQFVDLTATRPAQIFGLFPRKGTIAVGSDADLV
                     IWDPRRERTIRQAELHHAVDYTLYEGMAVRGAPRTVLRRGDLLVKDETFVGRPGTGEF
                     LRREKYRGRNAAPAA"
     gene            complement(1758..>3018)
                     /locus_tag="VHR64_00630"
     CDS             complement(1758..>3018)
                     /locus_tag="VHR64_00630"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEX3301358.1"
                     /translation="STGMSEAAGPRIEIDRQLVERFILELASFGAVGETGVSRPVYSP
                     AWVGATDRYAVWCRESGLDVRRDAVGNVSGVLAGSVGDGSIVSGSHIDSQSPGGRYDG
                     ALGVLGGLIAIRALSERFGRPRRTLEALALCEEEGSRFPSAGWWGSRAITGRIAPGDW
                     DRVVGYQDETIGDAMRRVGLDPERSADARRDDIAAFLELHIEQGPVLEQAGLPVAIVD
                     AITGIRHYAIDLGGTQNHAGAFPMDLRRDPMAGFAEIASGLIDTAHRLGRPAVTTIGR
                     VLVEPNFPAIIPAKISFMLDARHPDPTARERLYRTHERLMEEVAGRRGLDLSLRTVLD
                     HPPCICAPELVATLKAAAAAQGVPTMTMASGAGHDSQQMAAIAPVAMIFVRSKDGRSH
                     TPDEFSSIEDIVAGIEVLAAALHRLAY"
BASE COUNT          471 a         1018 c         1024 g          505 t
ORIGIN      
        1 tgctgacgcc ccggggtgca agagcgccgg tcctcgatac cgtactgcgg gcctcgtgcg
       61 gcaagctgat cgggtcgctg ggatcgtgcc tggcccgcat gccgcaagcg ccgatgccgt
      121 gctcgagcgg tgcgccttgg cgtccagcat tgggtcacca gactttggga atgctgttga
      181 tccgctccag gctgccgttc ccagtcgcga actcagtgaa gggccggacg gctttagatc
      241 gccgcgggct cgttccatcg tgcttcgaac ccggtcgagg accggcgctc ttgcaccccg
      301 gggcgtcagc aacaacgaac caagtgtctg gtcccatgtc aggcagccgg cgccgcattc
      361 cgcccgcggt acttctcccg acgcaggaat tcgccggtgc cgggtcggcc gacgaaggtc
      421 tcgtccttga ccaggaggtc gccgcgccgc agcacggttc gaggagcgcc ccgcacggcc
      481 atgccttcgt aaagcgtgta gtcgaccgcg tgatgcagct ccgcttgccg gatcgtccgc
      541 tcccggcgcg ggtcccagat caccagatcg gcgtcgctgc cgaccgcgat cgtgcccttg
      601 cgcgggaaca gaccgaagat ctgagccggc cgggtcgccg tcaggtcgac gaactgcggc
      661 aagcgcaggc ggccctggtc gacgccctgg tagaccatcg tcaggcgctc ctcgataccc
      721 ggggcaccgt tggggatctt gatgaagtcg tcgcggccaa gctccttgtc gccagcgtag
      781 ttccaggaag cgtggtcgga ggacaccagc tccagcgtcc ggtgtgccag cgcatgccac
      841 aggatctcgt ggtcctcccg cgtccgtgcg ggcggcgtga agacgtattt ggcgccttcg
      901 aagccgtccc gatcgaggtc ctccttggtg acgtagaggt aatgggtgca ggtttcggcg
      961 agcacgtcga cgccccggct gcgaccacgc acgacctcct ccagcgattc ccggcaggtc
     1021 agatggacga tgtagatcga ggtgccgacg agctcggcga gcgcgatcgc gcgcgcggtc
     1081 gcctcggcct cgacccgagg cggcctggtg ggggcgtgat atttgggcgc ggtcttgcct
     1141 tcggcgacgt actttcgctg caggaagtag gcggcatcgc cgttctcggc gtgcaccatg
     1201 accagcgccc cggccttgcg cgcctgctcc agcgcccgga tcaagaccag atcgtcgacc
     1261 ccgagcatgc ccttgtacgc catgaatagc ttgaagctcg tgattccttc gtcgggcagc
     1321 cgggcgagct cggcctcgac cgcatcgttc atgtcgcgca cgatgatgtg gaacccgtag
     1381 tcgatcgacg ccttgccggc cgccttctcg tgccagacac gcagcgcgtc ggcaagactt
     1441 tggccatgcg cctgctggca gaagtcgacg atcgtcgtgg tgcccccgca ggcggccccg
     1501 cgcgtcccgg attcaaagtc atctgcggtc acggtatcga agcccggggt gtcgagatga
     1561 gtatggacgt cgacaccgcc cggaatgaca tagcactcgc tggcatcgat gatccgggcg
     1621 ccggcggcct tcaggtcggc gccgatcagg gcgatcttgc cgccctcgac cgcgacgtcg
     1681 gccttcgaga tgccggcggc agtgactacg gttccgttct tgatcagggt atccatgacg
     1741 cggcctcctc aaaagcgtca ataggcgagc cggtgcagcg cggcggcgag cacctcgatc
     1801 ccggcgacga tgtcctcgat cgacgagaac tcgtcgggag tgtgactgcg gccgtctttg
     1861 ctgcgaacga agatcatcgc gaccggtgcg atcgccgcca tctgctgaga atcgtggccc
     1921 gcgccgctcg ccatggtcat ggtcggcacg ccctgcgcgg cggcggcggc cttcaaggtc
     1981 gcgaccagtt cgggagcaca gatgcagggc ggatgatcga gcacggtccg cagtgaaaga
     2041 tcgaggccgc ggcgcccggc gacctcctcc atcaggcgtt catgggtccg gtagaggcgc
     2101 tcgcgggcgg tcgggtcggg gtgccgggcg tcgagcatga agctgatttt ggccggaatg
     2161 atggcgggaa agttcggctc cacgaggaca cgtccgatag tcgtgaccgc ggggcgcccg
     2221 aggcgatggg ccgtgtcgat gagcccggat gcgatctcgg cgaagcccgc cattgggtcg
     2281 cgccggaggt ccatcgggaa ggcgccggca tggttctgag tgccgccgag atcgatcgcg
     2341 tagtggcgga tcccggtgat cgcatcgacg atcgcgaccg gaagacccgc ctgctcgagg
     2401 accgggccct gctcgatgtg cagctcaagg aaggcggcga tgtcgtcgcg ccgagcgtcg
     2461 gcgctgcgtt ccggatcaag gccgaccctg cgcatcgcgt cgccgattgt ctcgtcctgg
     2521 taaccgacca cccggtccca gtcgccgggc gcgatccggc cggtgatcgc cctcgacccc
     2581 caccagccgg ccgagggaaa acggctgccc tcttcctcgc agagggccag ggcctcgagc
     2641 gtccgcctgg gccggccgaa gcgctcgctc agcgcccgga tcgcgatcag gcctccgagc
     2701 acgccgagtg ccccgtcgta gcggccgccg ggtgattggg aatctatgtg cgatcccgag
     2761 acaatcgagc cgtcgccaac gctgcccgca aggacgcccg agacgttacc gactgcgtcg
     2821 cggcgcacgt ccagccccga ctcgcggcac caaacagcgt agcgatcggt cgctccgacc
     2881 catgctggcg agtagaccgg ccgcgacacg cccgtctctc cgaccgctcc aaacgaggcg
     2941 agctccagga tgaagcgctc gaccagttgc cgatcgatct cgatccgtgg tcccgccgct
     3001 tcgctcatac cagtgctg
//
LOCUS       DATAOZ010000026        12864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2384536, whole genome shotgun sequence.
ACCESSION   DATAOZ010000026 DATAOZ010000000
VERSION     DATAOZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12864
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2384536"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VHR64_00635"
     CDS             <1..882
                     /locus_tag="VHR64_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX3301359.1"
                     /translation="KPFAVVSNVAAGLNPDDAAALRSVGVPVLEGTETGLRAFRHLFI
                     YAEHRTTTTSLMQGPMTLAEDAIRLVRSNRALTEGESLSVLGELGMPAVTFRCARDEH
                     SAIEAARAIGFPAVMKTAVPGLLHKSDAGGVRLGIRDERELREAWQAMSARLGPDVLI
                     QPQLDTSQGVELVLGMGHDPQFGPMVTVGLGGVWVEALRDSITLLAPCSAGEVSRRLR
                     SLRGYDVLRGARGRVAVDIKALARVVEQFSLAVLALSAWVAEIDVNPLLAVGDQFTML
                     DALIVPFTAATGATSTG"
     gene            complement(848..1051)
                     /locus_tag="VHR64_00640"
     CDS             complement(848..1051)
                     /locus_tag="VHR64_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301360.1"
                     /translation="MVRRPPPEERSGPHHAIAICLAMALFFFLLWLGMTIIGDSARNS
                     ALIAFVLTMIFVFSLYWSPRSRR"
     gene            complement(1058..1351)
                     /locus_tag="VHR64_00645"
     CDS             complement(1058..1351)
                     /locus_tag="VHR64_00645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX3301361.1"
                     /translation="MFAIIARFKVRPGHVDEVIELLNQAASPSRAEAGCHFYVANRSL
                     DDPDLIVMYEQYESAEAFQAHIDTDHFREIVAGKVVPLLESRQRETYSVVTAG"
     gene            1411..2364
                     /locus_tag="VHR64_00650"
     CDS             1411..2364
                     /locus_tag="VHR64_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX3301362.1"
                     /translation="MSQSDAMIQPIRSELSVPGGDLRKVQKALASDADAVFLDLEDSV
                     APGQKDAARQVVIDMLTSLEWGLLEPAVRINGISTPWCYWDLIQIVEQAGSHLAKVVV
                     PKVRSAGDIHFVDRLLNQLEQRIGRTDPIRIEAQIEDAAGLMSIREIATASERISELT
                     FGQGDFAASVGMPAADIGVADEWDNVVEGDRWLLARQTIVFAARAAGIRALNGPYAAY
                     QSGEEFRGYCLMSRALGFDGVWCIHPNQIAIANEVFSPSEQEITRARATIEAMEDAWT
                     SGQGAASRDSVMIDEASLRMARSVLATAELIEQRQTKTDGA"
     gene            complement(2403..2843)
                     /gene="rpmE"
                     /locus_tag="VHR64_00655"
     CDS             complement(2403..2843)
                     /gene="rpmE"
                     /locus_tag="VHR64_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX3301363.1"
                     /translation="MKAGIHPNYQDATVVCACGNTFQTRSTRSTLRVDLCNVCHPFYT
                     GEQRIVDTAGQVERFMKKVETASTQARPSKRQQRIGQKSARLDEDRRRAEEATRAAAA
                     ERAAARKAQREAEAQVQAGAAASDVTEVAEVEIAEAPVEATAEA"
     gene            complement(2877..3173)
                     /gene="rpmA"
                     /locus_tag="VHR64_00660"
     CDS             complement(2877..3173)
                     /gene="rpmA"
                     /locus_tag="VHR64_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002088814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX3301364.1"
                     /translation="MAHKKGVGSSRNGRDSNAKRLGVKRTDGEFVLSGTIVVRQRGTQ
                     FWVGNNVGIGKDHTIYAKADGIVKFEPISRNKRRISVYPVTETATTVPTATAAD"
     gene            complement(3193..3594)
                     /gene="rplU"
                     /locus_tag="VHR64_00665"
     CDS             complement(3193..3594)
                     /gene="rplU"
                     /locus_tag="VHR64_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX3301365.1"
                     /translation="MTEDEARETTEAAEKPARSRSKKQDSAPANGYAIVETGGKQYRV
                     KVGDRIRVERLHADAGSDISLDRVLLLGGTGATTVGAPVVDGASVSAHIEDHLRGEKI
                     YVFKYKAKKRYRRKIGHRQELTELTITGINA"
     gene            3843..4583
                     /locus_tag="VHR64_00670"
     CDS             3843..4583
                     /locus_tag="VHR64_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3301366.1"
                     /translation="MDQRRTPKASVLVVDDEPSLRESIAYTLRREGFVVETAAEGTRA
                     LAMARERSPDLIILDIMLPGIDGLQICRTLRRESTVPIIMLSAKGEELDRVLGLEIGA
                     DDYVTKPFAMRELVARVRAQLRRVQLEEQRAAVPQPASGVIEFGDLRIDPASRKASVG
                     GRDLDLKPKVFDLLAYLASHPNQVFTRAQLLRDVWDYEVPIDTRTVDVHVRWLRQKLE
                     ESGGRVPEIETARRVGYRLTMSALPMSA"
     gene            4584..5939
                     /locus_tag="VHR64_00675"
     CDS             4584..5939
                     /locus_tag="VHR64_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3301367.1"
                     /translation="MSKRPIGSGFAIGFLIAGGAVGLIELILSTAFDWSRYSSVLMLF
                     IALIAGVAGGMAAIGWVQSWRDAVRSIGSPRANPAFLRRTRWPISLEAADDRVRLLTE
                     QRDNALAGQTFVQQRLEAIVSGLRDGVIVVSPDLSIVSINEAACRVLDVTQRHAVGQP
                     LVEIARDYDLVRIAQETVDSGHEQATPVDYRRLGRQLDVRVLPIEQAGRRLAVLVVQD
                     VTQLRALERMRTDFVSNVSHELRTPLAAIRALVETLNDGAIDDETVARDFLERVLGEV
                     DRLNELIEDLLDLGRLESGRLPLHRSEIAPAELIRRSVARVAHKAEDAGVEIRVFTAD
                     ELPILNVDASRLVQVLINLLDNAIKFSPRGGSVEVCAGEADGTVSIAVADHGAGIAPE
                     DLSRIFERFYKSDRSRHSGGTGLGLAIAKHIMSAHGGQLTATSEVGKGSVFTVSLPVT
                     E"
     gene            complement(5940..6890)
                     /gene="nikC"
                     /locus_tag="VHR64_00680"
     CDS             complement(5940..6890)
                     /gene="nikC"
                     /locus_tag="VHR64_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HEX3301368.1"
                     /translation="MSAEQAHSLTRRLRNDAEDTSMSLGEVGPLKKSPNQTLRNLRRF
                     IIGNRLNLLGLCIVVIFLFLSAFGSVIAPYDPAAKGDIIHSKLVGPSTDHLLGTDEQG
                     RDVFSRVLGGARDSLMVAVVVLTLSVIVGVIIGAISGYFGGMVDEIMMRITDMFLAFP
                     ALVLAIAIAATLGPSLRNTMLALATVFWPWYARLVRGQVLTIRERDYVDAARSIGLPS
                     SRILAKHVMPNAMSVVIIQVTLDIGYAILTTSSLSFVGLGAQPPSSEWGLMLSTARNY
                     FRDAWWYITFPGVALTLTVFAFNILGDGIQDALDPRSGRR"
     gene            complement(6887..7897)
                     /locus_tag="VHR64_00685"
     CDS             complement(6887..7897)
                     /locus_tag="VHR64_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301369.1"
                     /translation="MIVRLITRRLLFLVLVLFGLSIITFTISRIVPADPARMMLGPRA
                     SKAQVERQREAMGLNDPYPKQYVDYVWGVLHGDFGKSTSTRRNVSDDLRRYLPGTIEI
                     GLYAFILSTVLGVPLGVASAVRRDTLVDHISRFISITGLALPVFWLALMVQFLFYGTL
                     HILPDGQRLPQGVDPPKTITSLYTIDSLLHGQFGLFWTATQHLIMPVMVLTYGSLAVV
                     TRMVRGGMIEVMNQDYIRTARAKGLAQSTVVNRHALKNALLPTVTQLGLQIGLLLSGA
                     FLVEIIFSWPGIGRYSVDAIKQVDYNAIMATTLIVAVIFVLMNLVVDILYLFLDPRIS
                     YS"
     gene            complement(7966..9678)
                     /locus_tag="VHR64_00690"
     CDS             complement(7966..9678)
                     /locus_tag="VHR64_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3301370.1"
                     /translation="MSSNEISRFVDAASRGRMTRRQVLERGLGLGLSSALILSLMEAA
                     PEASASLAPAQKSFLIANQDNDPGVVTVCFVDATSDVDPHSSYTTYGAVVCLASYDML
                     INYKGLSTSEIEPMLAESWTASDDLTTYTFKIRANATFHDGTPCDAQAVKDSFIRFRR
                     LEMGPYLVIQRFCDDPENMITVPDATTVVFTLPKPEPLFLPAMASSYGPFVANPKRVE
                     ENKTDEDPWAHEWFVSNADGTGPYKLVENNLTEGMRFEKHDGYWGTFPEGGFHTVLVR
                     VVPEDATRRQLAEQGELDLLTNELTEEDNEALRSVPSLQVLTYPTTRVDWIIMNYVTL
                     TLEARQGLCWAFPYDEVIEGVYKGTVKRTGPIPDNVQGYDPDAFMYTTDLDKAKALFE
                     TAGLVNGDSITISIDSESEKSRTIAELFQANLSQIGVTLDISAVDTTTQEDIVYGDAP
                     AEEKPQLMGLWAWWPDYNEGWNQLAPNFLIESAGGGGSNGGYYENARNEELMAQARDA
                     ATSEESATMMKEIQDILIRQDPAAIFIGQVLYTTVADATIKGLEFNPLYIEQYFFHRY
                     YREA"
     gene            9907..10275
                     /locus_tag="VHR64_00695"
     CDS             9907..10275
                     /locus_tag="VHR64_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3301371.1"
                     /translation="MVKTATTDAVPATEDPRTESIGCPVARTANLFSNRWTPLILRDL
                     ADGSRRFGALQRSLVGISPKTLSERLKRLEESDVVARTCYAEVPPRVEYCLTDKGHAL
                     LPIIEAMRLYGSTWLVDEPC"
     gene            10362..11378
                     /gene="trpS"
                     /locus_tag="VHR64_00700"
     CDS             10362..11378
                     /gene="trpS"
                     /locus_tag="VHR64_00700"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX3301372.1"
                     /translation="MSSAPARKRVFSGIQPSGVSHIGNYLGAIRNWVLQQDIYDNVFC
                     IVNLHAITLPTEPQTLLENTLTMANTLLAAGIDPKRSIIFVQGDVREHSELCWMLNSV
                     TQYGELRRMTQFKDKAGGAEEGVSAALFDYPVLQAADILLYDADLVPVGEDQRQHVEL
                     TRDVAIRFNSRYGETFVVPAADIKAEGAKVMALDDPTKKMSKSSASVGSYISLTDTPD
                     EITKKFKRAVTDSGSEVRATADKPAITNLLTIHSLLSGMGIPELEEHFSGKGYGALKS
                     ELAEVVVTALRPIQERLAELAADPEETIAILAEGAHRARERACVKMAQVRERMGLELT
                     HSHR"
     gene            11382..12647
                     /locus_tag="VHR64_00705"
     CDS             11382..12647
                     /locus_tag="VHR64_00705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3301373.1"
                     /translation="MSAVGRIAGGYRETWHASGTGGRAILLGSGAFVARVVGFSFLLP
                     LYAKASGFSTEQYGLLVAIQNITPIIALIPVLVLARRGWDRRLMMLGPTIGMLGMLML
                     IAGRDAPYGVWMAAVILCGGSSATYWTLSDPLLAEATTPEFRAQAYALKRLFFTIGSS
                     VGALAAGAVPEGIQRSMGTTERDAYWVTLFVLAGLDGTQIWIFRRVPIRSLGHRMAGA
                     AGHHVPVSLLIGSLILFGLAEALFGFGYNSIRPFMSLFLTEREGLSSGTAGLVIASTA
                     IVAALGALLMPVLAERIGNARALGLLRFAGALSVTAWFAVGELGAVIALTLLYYGLLD
                     GTSALYAAEVMGRLPAAARDVMAGLNNVLWSVVGATAAALSGYLQDLPSGGFGLAFSV
                     GALGYLLSAAWCVIMLSRIALREVTPARS"
BASE COUNT         2358 a         3854 c         4127 g         2525 t
ORIGIN      
        1 aagccgttcg cggtcgtctc gaacgtggct gctggtctga atccggatga cgccgcggca
       61 ctgcggtcgg tgggagtacc ggtgctcgag gggaccgaaa ccggactgcg cgccttccgc
      121 catctgttca tttatgcgga gcatcggacg accaccacgt cgctaatgca agggcctatg
      181 acactcgcgg aggatgcgat tcggctcgtt cggtcgaatc gtgcgctgac ggaaggtgag
      241 agcctctcgg tgctcggtga gctcgggatg ccggcggtca catttcgctg tgcgcgagat
      301 gagcacagtg ccatagaggc ggctcgtgcc atcggatttc cggccgtcat gaagaccgct
      361 gtgcccggtc tgttgcacaa gtccgatgcc ggcggagtgc gtctcgggat tcgggatgag
      421 cgcgagctcc gcgaagcgtg gcaggcaatg agcgcgcggc tcggaccgga cgttctgatt
      481 cagccgcaac ttgacacgtc gcagggagtc gagctggtgc ttgggatggg ccacgacccg
      541 cagttcggcc cgatggttac ggtcgggctc ggtggtgtgt gggttgaagc gctgcgcgat
      601 tccatcacgc tcctggcacc gtgcagtgcc ggggaggtga gccggcggct gcggtcgttg
      661 cgcggatatg acgtattgcg cggagcacgc ggtcgtgtcg cggtggatat caaggcactg
      721 gcccgagtcg tcgagcagtt cagtctcgcg gtgctggcat tgtcggcgtg ggtcgccgag
      781 atcgatgtta acccgttgct ggccgtcggc gatcagttca cgatgctgga tgcgctgatc
      841 gtgccgttca ccgccgcgac cggggcgacc agtacaggct gaagacgaat atcatcgtaa
      901 ggacgaatgc gatcagagcc gagttgcggg cgctgtcgcc gatgatcgtc atgccgagcc
      961 agagcaggaa gaagaaaagc gccatcgcca ggcagatcgc gatcgcgtga tgcgggccgg
     1021 atcgctcttc gggcggcggt cggcgaacca aggcacctta ccccgcagtc accacggaat
     1081 aggtctcccg ctggcgagac tcgagcagag ggaccacctt gcccgcgacg atttcccgga
     1141 agtgatcggt gtcgatgtga gcctgaaatg cctctgcgga ttcgtactgc tcgtacatca
     1201 cgatcaggtc aggatcgtcg agtgagcgat tggcgacata gaagtggcag cccgcctcgg
     1261 cgcgggatgg actggccgcc tggttcagca gctcgatcac ttcgtcgaca tgtccgggcc
     1321 tgactttgaa tcgggcgatg atggcgaaca ccgatctgat cccttctact tcaaatcgat
     1381 tggggccgcg ttccgtgcga agattctcgc atgagccaat ccgatgcgat gatccagcca
     1441 atccggagcg agttgtcggt ccccggcggc gacctgcgca aggttcagaa ggcgctcgcc
     1501 tctgacgccg atgccgtgtt tctcgatctc gaggactcgg tcgcgccggg tcagaaggac
     1561 gctgcgcggc aagttgtcat cgacatgttg acgtcgcttg agtggggact tctggagccc
     1621 gctgtgcgca tcaatggcat cagcactccg tggtgctact gggacctgat ccagatcgtc
     1681 gagcaggcgg gatcgcacct cgcgaaggtc gtcgtcccca aggtgcgatc ggccggcgac
     1741 atccacttcg tcgaccggtt gctgaatcag ctcgagcaac gtattggccg gacggatccg
     1801 atccggatcg aggcgcagat cgaagatgcg gccgggttga tgtcaattcg ggagattgcg
     1861 accgcttcgg agcggatcag cgagttgacc tttgggcagg gagatttcgc cgcgtcagta
     1921 ggcatgcccg ccgcggatat cggcgtggca gacgaatggg acaacgtggt tgagggcgac
     1981 cgctggttac ttgcccggca aacgatcgtc tttgcggccc gcgccgcagg gatccgcgcg
     2041 ctgaatgggc cgtacgccgc ctaccagagc ggcgaggagt tccgaggcta ttgcctgatg
     2101 tcaagagctc tcggattcga tggcgtctgg tgcattcatc ccaaccagat tgcgatcgcc
     2161 aacgaggtct tctcgccctc cgagcaggag atcacacgag cccgggcaac catcgaggcg
     2221 atggaggatg cgtggacttc gggtcagggc gcggccagcc gggattcggt gatgatcgac
     2281 gaggcgagcc tgcggatggc ccgctcggtg ctggcgacgg ccgagctgat cgagcagcgg
     2341 caaacgaaaa cggacggagc atgagccccg tccgtctcgc ttggctttac ctgattgggt
     2401 gattaggcct cggcggttgc ctcgaccggc gcttcagcga tctcgacctc ggcgacctct
     2461 gtcacatcgc tggcagcggc cccggcctga acctgagcct cagcttctcg ctgtgccttg
     2521 cgagcggcgg cgcgctcggc agcggctgcc ctggtggctt cctcagcccg gcggcgatcc
     2581 tcgtcgaggc gcgcggactt ctggccgatg cgctgctggc gcttcgacgg gcgagcctgg
     2641 gtcgaagcgg tctcgacctt cttcatgaat cgctcgacct gaccagccgt gtcgacaatg
     2701 cgctgctcgc ccgtgtagaa cgggtggcag acattgcaga gatcgacgcg gagtgtcgac
     2761 ctggtggacc gggtctggaa cgtgttgcca catgcgcaga cgacggtggc gtcttgataa
     2821 ttcgggtgaa taccggcctt catttgtaac tccagaaagt tgccaggcag gccttgttag
     2881 tctgcggcag ttgcggtggg aactgtggtg gcggtctcgg tgaccgggta gacgctgatg
     2941 cggcgcttgt tccggctgat cggctcgaac ttcacgatgc catcggcctt ggcgtagatc
     3001 gtgtgatcct tgccaatgcc gacgttgttg ccgacccaga actgcgtgcc gcgttggcgc
     3061 accacgatgg ttccgctcaa aacgaattct ccgtcggtgc gcttgacgcc gagccgcttg
     3121 gcattgctat cgcggccgtt acgtgagcta ccgacacctt tcttgtgggc catttcgtat
     3181 ctctccttcg gcttaggcgt tgatgccggt gatcgtcaac tcggtcaact cttgccggtg
     3241 accgatcttg cggcggtatc tcttcttcgc cttgtacttg aagacataga tcttctcgcc
     3301 gcgaaggtga tcctcgatat gcgctgagac cgaagcaccg tcgacgaccg gcgcgccgac
     3361 cgtggtcgcg cccgttccgc cgagcaggag cacgcgatcg agcgaaatgt cgctgccggc
     3421 atcggcatgg aggcgctcga cacgaatgcg atcgccaacc ttgacgcgat actgcttgcc
     3481 gccggtctca acaatggcgt agccgttggc cggcgccgaa tcttgcttct tcgaccgcga
     3541 gcgggccggc ttctcggccg cctcggtcgt ttccctggct tcgtcttcgg tcatctgctg
     3601 gtatccaaat caacgcacaa aatggcgagc agccaacaaa aaagggctcc tcgctcccac
     3661 aagctgaaat aatagcacag gtgtcgaggc tgaccgaagg tgcgtcgctg gctactgaag
     3721 gcaagacgga aacttaccgt ccgttaacaa tagcgcgcca ttcgggccat gccgcttacc
     3781 tatacttaac atacggtaac ggtttctgtc cgtgaggcag cagagtggtt cagcccggtg
     3841 taatggatca acgaagaact cccaaggcat cggtcctcgt cgtcgatgac gagccgtcgc
     3901 ttcgcgagtc gatcgcgtac accctgcgcc gcgaagggtt cgtggtcgag accgctgccg
     3961 aagggacgcg cgcgctcgcc atggcccggg agcgatcccc cgacctgatc atcctcgaca
     4021 tcatgttgcc gggaatcgac gggctgcaga tctgtcgcac gcttcgccgg gaatcgaccg
     4081 ttccgatcat catgctctcc gcgaagggcg aggagctgga ccgggtgctc gggctcgaga
     4141 tcggcgcaga cgattacgtg accaagccat ttgcgatgcg cgagttggtg gcgcgcgtcc
     4201 gtgcgcagct gcgccgggtc cagctcgagg agcagcgggc agcggttcca caaccggcga
     4261 gcggtgtgat cgagtttggc gatctccgga tcgatcccgc cagtcgcaag gcgagtgtcg
     4321 gcggccggga tctggacctg aaaccgaagg tgttcgacct gctggcatat ctcgcgagcc
     4381 accccaatca ggtattcacc agggcgcaat tgctccggga cgtctgggac tacgaggtgc
     4441 cgatcgacac gcgcactgtt gacgtccacg tgcgctggct gcggcagaag ctggaagaga
     4501 gcggtggccg ggtgccggag attgagacgg ctcggcgcgt cgggtaccga ttgacgatga
     4561 gcgcattgcc gatgtccgcc taaatgagca aacgaccaat cggttccggg ttcgctatcg
     4621 gctttctgat tgcgggcggc gcggtcggtc tgattgagct gatcctctcg acggccttcg
     4681 actggtcgcg atattcatct gtcctgatgc ttttcattgc cctgatcgcc ggtgtggccg
     4741 gcggcatggc ggcgatcggc tgggtacaga gttggcgcga tgccgtccgc agcatcggat
     4801 cgccgcgcgc aaaccccgca ttcttgcgcc gcacccgctg gccgatctcc ctcgaggcgg
     4861 ctgacgatcg ggttcgcctg ctcaccgaac agcgcgataa cgccctcgcc gggcagacct
     4921 tcgttcagca acgactcgaa gcgatcgtgt ccgggctgcg cgatggcgtg atcgtggtga
     4981 gcccggatct ctcgatcgtc tcgatcaatg aggcggcctg ccgggtgctg gatgtcacgc
     5041 agcggcatgc tgtcggccag cccttggtcg agattgcccg cgactacgac ctcgtccgga
     5101 tcgcccagga gacggtggac agcgggcacg agcaagcgac gccggtcgac taccggcgat
     5161 tggggcgcca gctcgacgtg cgggttctgc cgatcgaaca ggctggacgc cgactcgcgg
     5221 tgcttgtcgt ccaggacgtg acccagctgc gggcactaga gcgaatgcgg acggacttcg
     5281 tctctaacgt ctcgcacgaa ttacggaccc cgctcgcggc gatccgcgcg ctcgtcgaga
     5341 cactcaacga cggcgcgatc gatgacgaga ccgtcgcgcg ggattttctg gaacgcgtgc
     5401 tgggcgaggt cgaccgactc aacgagctga tcgaggacct gctggatctc ggccggctgg
     5461 agtccggccg gttgccactc caccggtcgg agatcgcccc ggccgagctg attcggcgct
     5521 ccgttgcgag agttgcccac aaagccgagg acgccggtgt cgagatcagg gtgttcacag
     5581 ccgacgagtt accgatcctg aacgtagacg cctcccggct ggtccaggtg ctgattaacc
     5641 tgctggacaa cgcgatcaaa ttctcgcccc gtggtgggtc agttgaggtc tgcgccgggg
     5701 aggccgacgg tacggtatcg atcgcggtcg ccgatcatgg cgccggcatc gcgcccgagg
     5761 acctttcccg catcttcgag cgtttctaca agtcggatcg ttctcgccat tcgggcggca
     5821 ccggactcgg tctcgcgatc gcgaagcaca tcatgagcgc acatggcggg caattgacgg
     5881 cgacgagcga ggtcgggaag ggctcggtct tcaccgtcag cctgcccgtg actgaatgat
     5941 cagcgccgac ctgagcgtgg gtcgagtgca tcctggatgc cgtcgcccag gatgttgaag
     6001 gcaaagaccg tcagggtgag cgcgacgccc gggaaggtga tgtaccacca ggcatcccgg
     6061 aagtagttcc gcgcggtcga gagcatcagg ccccattcgg acgacggcgg ctgcgcgccg
     6121 aggccaacaa agctgagcga gctggtcgtc agaattgcgt acccgatatc cagcgtcacc
     6181 tgaatgatca cgaccgacat cgcgttcggc atgacgtgct tggcaaggat ccgcgacgac
     6241 ggcaatccga tagatcgggc cgcatcgaca taatcccgct cccggattgt cagcacctga
     6301 ccgcgcacga gccgggcgta ccacggccaa aagaccgtcg ccagggccag cattgtattg
     6361 cgaaggctgg gcccaagcgt cgccgcgatc gcgatcgcga ggacaagcgc tggaaacgcc
     6421 aggaacatgt ccgtgattcg catcatgatt tcgtcgacca tgccgccgaa atatccggag
     6481 atcgccccga tgatcacgcc gactatcact gatagggtca ggacaaccac ggcgaccatc
     6541 agcgagtcac gggcgcctcc aagcacacgg ctaaagacgt ctcgtccctg ttcgtcagtc
     6601 ccgagcaagt gatcggtgga tgggccgacc aacttcgaat ggatgatgtc gcctttggca
     6661 gccgggtcgt acggggcgat cacactgccg aatgctgaaa gaaagagaaa gatcaccact
     6721 atgcaaaggc cgagcaggtt gagacgattg ccgattatga accgccgaag attccgcagc
     6781 gtctgattgg gcgacttttt cagcggccca acttcgccga gtgacatcga cgtgtcctcg
     6841 gcatcgttgc ggagtcgccg ggtcaggctg tgtgcctgtt cagcgctcat gagtagctaa
     6901 tccttgggtc caggaaaaga tacaggatgt ctaccaccag gttcatgagg acgaagatca
     6961 ccgccacaat caacgtcgtc gccatgatcg cgttgtagtc aacctgcttg atagcgtcga
     7021 ccgagtatcg cccaatgccc ggccaggaaa agatgatctc gaccaggaac gcgcccgaga
     7081 gcaagagccc gatttgcagg ccgagctgcg taacagtcgg aaggagagca ttcttcagcg
     7141 catgtcggtt gacgactgtc gactgggcaa gccccttggc tcgggcggtt cgaatgtaat
     7201 cctggttcat cacctcgatc atgccgccgc gaaccattcg cgtgaccact gccagcgacc
     7261 cgtatgtcag caccatgacc ggcatgatga gatgctgcgt tgccgtccaa aacagcccga
     7321 attgtccatg gagcagggag tcgatcgtgt acagagaagt gatggtcttc gggggatcga
     7381 caccttgcgg cagccgttga ccgtccggca agatatggag cgttccgtaa aagaggaact
     7441 gcaccatcag cgcgagccaa aagaccggga gtgctaagcc cgtaattgag atgaaccgag
     7501 agatgtggtc gactaacgtg tcacgtctga cggctgaggc aacgcccagg gggacaccga
     7561 gaaccgtcga caagatgaac gcgtacagac cgatttcgat cgttccgggc aggtatcttc
     7621 gcaggtcgtc gctcacgttc cgcctggtgc tcgtgctttt gccgaaatcc ccgtggagca
     7681 ccccccagac atagtcaacg tactgtttgg gatacggatc gttgagaccc atggcttctc
     7741 gttgtcgctc gacctgcgcc ttgctcgccc gcggtccgag catcatccgt gctgggtcag
     7801 ccgggacgat ccgcgagatc gtgaatgtaa tgatcgagag cccgaatagc acgagcacca
     7861 agaagagcaa tcgtctcgtg ataagtcgga cgatcatttg cggggaacct ccgggttacc
     7921 atgggcggcc gacattcggc cgcccatgaa agcgaggccc agggcctatg cctcgcgata
     7981 gtaacgatgg aagaagtact gctcgatata aagcgggttg aactctagtc ccttgatcgt
     8041 tgcgtcggca acggtggtat agagcacctg cccaatgaag atcgccgccg gatcctgtcg
     8101 aatcaggata tcctgaatct ccttcatcat cgtcgcgctc tcttcgctcg tggccgcgtc
     8161 ccgagcctgt gccatcagct cttcgttgcg ggcgttctcg tagtatccac cgttgctgcc
     8221 accgccgccg gcggattcga tgaggaagtt gggggccaat tggttccagc cctcgttgta
     8281 gtccggccac caagcccaga gacccatcag ttgcggtttc tcttccgctg gggcatcgcc
     8341 atacacgatg tcctcctgcg tcgtagtatc gaccgccgat atatccaacg tcacaccaat
     8401 ctgcgacagg tttgcctgga acaattcggc aatggtgcgc gacttttcgc tctcgctgtc
     8461 gatgctgatc gtgattgagt cgccgttgac aagcccagct gtttcaaaca gggctttagc
     8521 cttgtcgagg tccgttgtgt acatgaacgc gtccggatca tatccttgaa cgttatccgg
     8581 gatcggtcca gtgcgcttca cagtgccctt gtagactccc tcgatgacct cgtcatatgg
     8641 gaatgcccag caaaggccct gacgcgcttc gagcgtcagg gtcacgtagt tcatgatgat
     8701 ccagtcgact cgggtcgtcg ggtatgtgag gacttgaagc gaaggaacgc tgcggagcgc
     8761 ttcattgtct tcttccgtca actcgttagt cagcagatcg agctcgccct gttcagcaag
     8821 ttgtcggcgc gttgcgtctt ccggcacgac tcgaaccagt accgtgtgga atccgccttc
     8881 agggaacgtg ccccagtacc cgtcgtgctt ttcgaagcgc atgccctcgg tcaggttatt
     8941 ctccaccagc ttgtacgggc cagtgccgtc ggcattggaa acgaaccatt catgcgccca
     9001 gggatcctcg tccgtcttgt tttcttcgac ccgctttggg ttagcaacga acgggccata
     9061 cgatgaggcc attgcgggca ggaagagtgg ctccggtttc ggcaaagtga agactacggt
     9121 cgtcgcatcc ggaacggtga tcatgttttc gggatcgtcg cagaatcgct ggataactag
     9181 gtatggtccc atttctagcc ggcggaaccg aatgaacgag tctttcacgg cttgggcatc
     9241 gcagggcgtt ccgtcgtgaa acgtcgcgtt cgcccggatc ttgaacgtgt acgttgtcag
     9301 atcgtcgctg gctgtccagg actcggcgag catcggttcg atctcggagg tcgatagacc
     9361 cttgtagttg atcagcatgt cgtacgaggc gaggcaaacc accgcgccat atgtggtgta
     9421 tgacgagtgc gggtcgacat cgctcgtggc atcgacgaaa cagacggtaa ccacgccggg
     9481 atcgttatcc tgatttgcga tcaggaacga cttctgtgct ggcgccaggg aggctgaggc
     9541 ttcaggggcc gcctccatca gtgagaggat cagagccgat gacaggccca ggccgagccc
     9601 tcgctcaagg acctggcgac gtgtcatgcg acctctcgac gcagcgtcga cgaatcggga
     9661 gatctcattc gaactcatga accgggttcc tttcctggct ctcgtgccgt ttgccacatt
     9721 tacgacccgt cgatcaccag ggttctttgc ccggaagatc tcgacaggtg tccaaagtgt
     9781 gtatgccccg gaactatacg gaccggaaag atttcacgcc aaccatttga atcgatatcg
     9841 ggcgggcttg caggccgtgc tatcctttgg gaagtatcat tattggcgct gaaacggtag
     9901 ttcagcatgg tcaaaaccgc gacaactgac gctgttccgg cgaccgagga tccccgcaca
     9961 gaatccatcg gctgcccggt ggcgcgcacg gcgaatctct tcagcaaccg ctggaccccc
    10021 ctgatcctgc gagacctcgc ggatggctca cgacggttcg gcgcacttca gcgctccctg
    10081 gtcgggatca gccccaagac cctctctgag cggctgaagc ggctcgagga atccgatgtc
    10141 gtggcgcgca cctgctacgc cgaagttcca ccgcgcgtgg aatactgcct gaccgacaaa
    10201 ggccacgctc tcctgccgat catcgaggcg atgcggctct acggctcgac ctggctggtc
    10261 gacgagccct gctagattcc gaccgcccat cggtataatc aggccgcgtc gctccggacg
    10321 cccgaaatct atcccgagca ccctgagagg tttcattcaa catgtcgagt gcgcctgcac
    10381 ggaagcgcgt cttctccggt atccagccct ccggcgtcag tcatattggc aactatctgg
    10441 gtgcgatccg caactgggtg ctgcagcagg acatctacga caacgtcttc tgcatcgtga
    10501 accttcacgc gatcacgctg ccgaccgagc cgcagacgct gctcgaaaac acgctgacga
    10561 tggcgaatac cttgctggcg gccgggatcg acccgaagcg gtcaatcatt ttcgtccagg
    10621 gcgatgtccg ggagcattcc gagctgtgct ggatgctgaa ctcggtgacc cagtacggcg
    10681 agctacgccg gatgacccag ttcaaggaca aggcgggcgg ggcagaggag ggcgtctcgg
    10741 cagcgctgtt cgactacccg gtgctgcagg cggccgacat cctgctctac gacgccgacc
    10801 tggtgccggt gggcgaggat cagcgccagc acgtggagct gacccgggac gtcgcgatcc
    10861 gctttaactc gcgctatggc gaaacattcg tcgtacccgc cgctgacatc aaggcagagg
    10921 gcgcgaaagt gatggcgctc gacgatccaa ccaaaaagat gagcaagagc tccgcatcgg
    10981 tcggctccta catctccctg accgacacgc cggacgaaat tacgaagaag ttcaagcgtg
    11041 cggtcaccga ctccggaagc gaagtgcggg cgacggccga caagccggcg atcaccaatc
    11101 tcctgaccat tcattccttg ctgtcgggca tgggcatccc ggaactcgag gagcacttca
    11161 gcggcaaagg ctatggcgcg ctcaagagcg agctggccga agtcgtggtg acggcgctgc
    11221 gcccgatcca ggagcgattg gccgagcttg cagccgatcc ggaggaaaca atcgctatcc
    11281 tggccgaagg cgcgcaccgg gcccgtgagc gggcgtgcgt gaagatggcc caggtgcggg
    11341 agcgcatggg gctcgaactc acccactctc accggtagtt ggtgagcgcg gtcgggcgta
    11401 tcgccggtgg ctaccgggag acgtggcacg cgagcggcac gggtggccgg gccattctgc
    11461 tcgggagcgg ggcgttcgtc gcccgcgttg ttggcttctc gttcctgctt ccgctctacg
    11521 cgaaggcctc cggattcagt accgagcaat acggcctgct ggtcgcgatc cagaacatca
    11581 ccccaatcat tgccctgatc cccgtgctcg tccttgcgcg gcgcgggtgg gatcgccgcc
    11641 tgatgatgct gggaccgacc atcggcatgc tcggcatgtt gatgctgatc gccggtcgcg
    11701 acgcgccata cggagtctgg atggcggctg tgatcctgtg cggcgggagc tcggccacct
    11761 attggacgct gagcgatccg ctgctcgcgg aggcgaccac gccggagttc cgggcacagg
    11821 cgtacgcgct caaaaggctt ttcttcacga tcggatcgtc cgtcggcgcg ctcgccgccg
    11881 gtgcggttcc tgaaggcatc caacgatcga tgggcacaac cgagcgggat gcctactggg
    11941 tcacgctctt cgtgctggcc gggctggatg gcacgcagat atggatcttc cggcgagtgc
    12001 caatccgatc gcttggccac cggatggcag gcgccgccgg gcatcatgtg ccggttagcc
    12061 tgctgatcgg gagcctgatc ctcttcgggt tggccgaggc gctcttcgga tttggctaca
    12121 acagcatccg gccgttcatg tcgctcttcc tgacggagcg cgaggggctg tcgtccggaa
    12181 cagcgggcct ggtcatcgcc tctaccgcga tcgttgccgc ccttggcgca ctgttgatgc
    12241 cggtgctggc tgaacgtatc ggcaatgcgc gggcactcgg gctgctccgg tttgccggcg
    12301 ccttgtcggt cacggcgtgg ttcgcggtcg gagagctcgg cgccgtgatc gcactgacgc
    12361 tgctctacta cggcttgctg gatgggacca gcgcgctcta tgcggcggag gtgatgggca
    12421 ggttgcctgc cgccgcgcgc gatgtcatgg ccgggttgaa caacgtcctg tggagtgtgg
    12481 tcggcgcgac ggccgcagcg ctgagcgggt acctgcagga tctgccctcg ggcggcttcg
    12541 ggttggcctt ctcggtcggg gcgctcgggt atctgctatc ggctgcctgg tgcgtgatca
    12601 tgctgtcccg cattgccttg cgggaagtga cgccggcgcg gagttaagac ggggcctgag
    12661 gctgtgcgta cccaaacgaa tgtagcaccg ggcggctcgc ccggtccgcg gctcacggga
    12721 tgccgggcga gccgcccggt gctacatcag acgaacgacg ctttttcaca gacaagctct
    12781 aacccgcgaa tcggcgtgcg atgcgattgg atgcgcgctc atcctgcttg aggagcagac
    12841 cgcaccagat tgtgagcaga ccgg
//
LOCUS       DATAOZ010000027         5819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6330200, whole genome shotgun sequence.
ACCESSION   DATAOZ010000027 DATAOZ010000000
VERSION     DATAOZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6330200"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VHR64_00710"
     CDS             <1..319
                     /locus_tag="VHR64_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301374.1"
                     /translation="VDVRVGMDGYHIEMWASSPGMFADEENEWKRRAQQRLVRIMLPN
                     IARGHLAENQHAYWDDVDQGVSVRVTYELPFTRAEQIGQFVRQHLPELEDLLTLVESQ
                     IEI"
     gene            394..2067
                     /gene="nadB"
                     /locus_tag="VHR64_00715"
     CDS             394..2067
                     /gene="nadB"
                     /locus_tag="VHR64_00715"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX3301375.1"
                     /translation="MNQASFTAGSPTPLITPDSADRRSQVDVIVIGAGVAGLAFALSI
                     PAELRVALLTKGNLGESNTRWAQGGLSAAIGADDTIELHEQDTLYAGAGLSDPETVHE
                     LVADGPDAVDWLLSLGTAFDRDEESGELALGREAAHSRRRVLHAGGDATGAEIERALV
                     AAVRSRPNVQILECGFAIDPFVRDGACSGALISLQNREIVALGSQAVVLAAGGAGQLW
                     ATTSNPPAATADGLAMAIRAGAAVADLEFCQFHPTVLQLNGETPFLVSEAVRGEGAYL
                     RDHAGNRFMVAEHELAELAPRDVVARGIQKQMAADGADHVFLDLRHLDGPTMRRRFPT
                     IARELDERGLDLATDLIPVAPAAHYFMGGVVADEFGVTSLPGLLAVGEAACTGVHGAN
                     RLASNSLLEGLVFGRKAATALRFSPIGEVAAFDDRAASTTADDLSADLASIRSAIRQT
                     MSRHVSVVRDAAGLTTAIAELDQITRRLPDKPSGRAEWVTANMVMAARAIATAALLRE
                     ESRGAHFRSDFPDRDPGLDGLHYICHSGLPEEGRWTLGTLSSAIPVVRT"
     gene            2064..2696
                     /locus_tag="VHR64_00720"
     CDS             2064..2696
                     /locus_tag="VHR64_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301376.1"
                     /translation="MSWDAVLERWSSETLLFIPALPGFQVNAESPEDALADAEERVDA
                     YIQWLRDGELLPENFPSPEGIAIAETRVANGEAGPIFDLDTEPIDEDFAEFALAIGRA
                     ALSDLLFVFDDVPDEGRSEAERILRHVAELDRWYATRMAPAQGKPFTAIEDELVQSAS
                     LFEETVDGVMNSRQPTTWTLNGEDWSIRKALRRRTGHLREHLADLLSLTR"
     gene            2701..4020
                     /locus_tag="VHR64_00725"
     CDS             2701..4020
                     /locus_tag="VHR64_00725"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX3301377.1"
                     /translation="MTESATEIIHRRSALARGLVADRSPDQAVAERGLHRMGALLAAL
                     GNPQRAYRVIHVAGSKGKGTTTHVAAAVLTALGYRTGRYVSPHLSSWNERIAIDGQSI
                     PDFEFASILTEVDRTMTDLEVRGPELGTFNAFELLTAAAFVYFRDQRCDVAVIEVGLG
                     GRFDSTNHVSPEVSVITRIEAEHLDVLGPTIEQVAWNKAGIIKPGVPVVIAMQQSEVE
                     AVLELQAGDIGAPLLREGTEWTVTSDSKVLCYHVRDEQPLKLISAIPGRHNRSNIGAA
                     LTAVHLLPGNQPLNGDLLSNVLNSIRIPGRFDRRVDPVTGRTIILDGAHTPESIRALV
                     ETMHAEFGPARFPVIMGLLVDKPANEILSILAPAVSRIIFPELEGPRIVRAQSLDEAA
                     VRLDMQASIRPTIRAALDEFGPGSEPVLITGSFGIVAAALVDLSMTP"
     gene            4156..4797
                     /locus_tag="VHR64_00730"
     CDS             4156..4797
                     /locus_tag="VHR64_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3301378.1"
                     /translation="MAIATNPTEPLLTAESAEDAILVARLAEGDGNALEVLYDRYSRV
                     VYSFSLRMLADASSAEELTQEVFLRLWRQGGSYQQSRGAFLTWLLSVTHNMAIDEIRR
                     RKRRPVAAETSEDDQTLTMLPDTRTDVEGAAWLTTLQAIVRDALQEIPQPQRQVIELA
                     YFSGLTQREISDRLNEPLGTIKTRMRLGLLKLRDRLGPILDDDVSAPANKETE"
     gene            4794..5705
                     /locus_tag="VHR64_00735"
     CDS             4794..5705
                     /locus_tag="VHR64_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301379.1"
                     /translation="MNRYDDRTPHMTTRQATVDCDWVREHAPVHALGALESDVADQLE
                     SHLLLCQECDRELASLMRVSALLGAGVTQVEPPASIKRNLMAAIATPVLAAESTTSML
                     PEIPAAAASPAPLASPAHAWMSSRALFAPILGALLLLSLWTAHTHQQLSSQRDQVSHL
                     QRQNDALTVHLSSLQAGQQFFGSSGFWMPLSVVDANATEAGGIVMSGDQNTTTLLSVW
                     NMPDDHDTYHVLCESKRGELLTAGEIRVNANGSGTVTLNLPSPVTEYRAVHVVPMESA
                     TYEADILTHDILQLLIGEPTAVATNET"
BASE COUNT         1079 a         1865 c         1831 g         1044 t
ORIGIN      
        1 ggtcgatgtc cgggtcggca tggatggcta ccacatcgag atgtgggcct cgtcgccggg
       61 gatgtttgcc gacgaggaga atgagtggaa acgacgcgcg cagcagcggt tggttcggat
      121 catgttgccg aacatcgccc gcggtcacct cgccgagaat cagcacgcat actgggacga
      181 cgttgatcag ggcgtctccg tgcgagtgac atatgagctc ccgttcaccc gcgccgagca
      241 gatcgggcag ttcgtgcggc agcatctgcc tgagctcgaa gatttgctga ccctggttga
      301 atcgcagatc gaaatctgag actgaccagc ctacgatccg gtatcgttca acctgatgaa
      361 cacatccctt cccaaagcac gaggcaacgc ccgttgaacc aggcatcttt cactgcaggc
      421 tccccgaccc cgctgatcac accggattcc gcggaccgtc gttcgcaagt cgacgtcatt
      481 gtcattgggg ccggcgtggc cggcctcgcc ttcgccctgt cgatacccgc cgagctaagg
      541 gttgccctgc tgaccaaggg aaacctcggc gagagcaaca cgcgctgggc ccagggcggg
      601 ctgtcggcgg cgatcggtgc ggacgacacc atcgagctgc atgagcagga caccctctac
      661 gccggcgccg ggctcagcga tcctgaaaca gtccacgagc tggtggccga tggcccggac
      721 gcggtcgact ggctgctctc gctcgggacc gccttcgatc gcgacgaaga gtccggcgag
      781 cttgccctcg gtcgcgaggc tgctcacagt cgccgccgcg ttcttcacgc cggaggggat
      841 gcaaccggcg ccgagatcga acgcgcgctc gtcgcggcag ttcgatcccg cccgaatgtc
      901 cagatccttg agtgtggctt cgcgatcgac ccgttcgttc gagatggagc ctgctcgggt
      961 gctctgattt ccctgcaaaa tagggagatc gttgcgctcg gatcccaggc agtggtgctg
     1021 gcggccggcg gtgcgggtca gctctgggcg acgacgtcga acccgcctgc agcaaccgca
     1081 gacggcctgg cgatggccat tcgcgccggc gccgctgttg ccgatctcga gttctgccag
     1141 ttccacccga ccgtcctgca actgaatggc gagactccat tcctggtatc cgaagccgtc
     1201 cggggtgagg gcgcatatct ccgggaccac gccggcaacc gtttcatggt cgccgagcat
     1261 gagctggccg aactggcccc gcgagacgta gtcgcgcgcg gtatccagaa gcagatggcg
     1321 gctgatgggg ccgaccacgt cttcctcgat ctccgccacc tcgacggccc gacgatgcga
     1381 cgccgatttc cgactatcgc ccgcgagctc gatgagcgcg gtctcgatct cgccacggac
     1441 ctgatcccgg tcgcaccggc cgcgcactac ttcatgggtg gcgtcgttgc ggacgaattc
     1501 ggcgtgacta gtctgccggg gttgttggcg gtcggcgaag cggcctgcac aggtgtacac
     1561 ggcgccaacc ggcttgccag caactcgctg ctcgaaggac tggtcttcgg gcgaaaagcg
     1621 gcgacagctc tgcgattttc accaatcggt gaggtagccg cattcgatga tcgggctgcg
     1681 agcaccactg ccgatgattt gtcggcagat ttggcctcaa tccggtcggc gatccggcag
     1741 accatgagcc ggcatgtctc cgtcgtacgc gacgccgccg gattgacgac cgcgatcgcc
     1801 gagctggacc aaatcaccag gcgactcccg gacaagcctt ccggccgagc cgaatgggtg
     1861 acggccaaca tggtcatggc agcgcgggca atcgcgaccg ctgcgctgct ccgtgaggag
     1921 agccgcggcg cccatttccg atccgacttc ccggatcgcg atccggggct ggacggcctg
     1981 cattacatct gccattcggg tttgcccgaa gagggtaggt ggacgctcgg cacgctgtcg
     2041 tcggcgatcc cggtggtgcg gacatgagtt gggacgcggt tctcgagcgc tggtcgagcg
     2101 agacgctttt gtttatcccg gcgttgcccg gctttcaggt caatgccgaa tctccggaag
     2161 acgcactggc cgatgcggag gagcgggtcg acgcctatat tcaatggctc cgcgacgggg
     2221 agctgctgcc ggagaacttc ccatctcccg aaggcatcgc gattgccgag acgcgagtgg
     2281 ccaatggcga ggctggtccg atcttcgatc tcgataccga gccaatcgac gaggacttcg
     2341 ccgagtttgc gctggcaatc ggtcgcgccg cgctctcgga tttgctcttc gtcttcgacg
     2401 atgtgccaga tgagggacgt tccgaggccg agcgtattct gcgccacgtg gccgaattgg
     2461 accgctggta cgcgacgcgg atggcgccgg cccaggggaa gccgttcacg gcgatcgagg
     2521 atgagctcgt gcagtcggcc agcctcttcg aggagaccgt ggacggcgtg atgaactcac
     2581 ggcaaccgac gacctggacg ctgaacggtg aggactggag cattcggaag gcgctgcggc
     2641 gacgcaccgg tcaccttcgc gagcacctcg ccgacctgct cagcctgacc cggtagaccc
     2701 gtgaccgaat cagcgaccga gatcatccat cgccgcagcg ccctcgctcg gggtctggtg
     2761 gccgaccgtt cgccggacca agccgtcgcg gagcggggat tgcaccggat gggcgcgctt
     2821 ctcgcggcgc tcggcaaccc gcagcgtgcc taccgcgtga ttcacgttgc gggaagcaaa
     2881 ggcaagggca cgacgacgca cgtagccgcc gccgtcttga ccgcgctggg atatcgaacc
     2941 ggccgctacg tctcgccaca cctgtcgtcc tggaatgagc gcatcgcgat cgacggacaa
     3001 tccatccccg atttcgagtt cgcctcgatc ttgaccgagg tcgaccgaac gatgacggat
     3061 ctcgaggtcc ggggtccgga actgggcacc ttcaacgcct tcgagctgct gaccgctgcg
     3121 gctttcgtct atttccggga tcaacgctgc gatgtcgcgg tgatcgaagt cggtctgggt
     3181 ggtcgcttcg attcgacgaa tcacgtcagt ccagaggtca gcgtgattac ccgaatcgaa
     3241 gccgagcacc tcgatgtcct cggcccgacc atcgagcaag ttgcctggaa caaggcggga
     3301 atcatcaagc ccggcgtccc ggtcgtgatt gcgatgcagc aatcagaggt tgaggctgtc
     3361 cttgaattgc aggcagggga catcggagcg ccgctgcttc gtgaaggcac ggagtggacg
     3421 gtgacttcgg acagcaaagt gctctgctat cacgtcagag atgagcagcc cctgaagctc
     3481 atctcggcaa ttccaggacg gcacaatcgc agcaatatcg gcgcggccct aaccgcggtt
     3541 cacttgctcc ccggcaatca accactcaac ggcgacttgc tatcgaatgt gctcaactca
     3601 atccggattc cgggtcgctt cgatcggcgt gtcgatccag tgactggtcg aacgatcatc
     3661 ctcgacggcg cgcacacacc cgagtcgatc cgggcacttg tggagacgat gcacgccgag
     3721 ttcggaccgg cgcggtttcc ggtgatcatg ggcctgcttg tcgataagcc ggccaacgag
     3781 attctctcga tcctggcgcc tgctgtctcc aggatcatct tcccggaact tgagggcccg
     3841 agaatcgttc gggcgcaatc actcgacgaa gccgccgtcc gacttgacat gcaggcatcg
     3901 atccggccga caatcagggc ggcgctcgat gaattcgggc caggatcgga gccggtgctt
     3961 attaccgggt cattcgggat tgtggccgcg gcattggtcg atttgtcgat gactccgtaa
     4021 ccaaggtact ggcgcgtggg tttaggatta gtacaagatc gtccggaacc tgccgggcga
     4081 gacgcaaaac cgcaacggcc gaacccgacg tacacagggg aagggaagag gcccggcggt
     4141 acacggattc cgtcgatggc aattgccacg aatccaaccg agcctctcct cacggccgag
     4201 tccgcggaag acgcaattct ggttgcgcgc ctagccgagg gagatggcaa cgcactggaa
     4261 gtgctgtacg accggtacag ccgcgtcgtc tactcgttct cactccgcat gctcgcagac
     4321 gccagctcgg ccgaagagtt gacgcaggag gtcttcctgc gtctttggcg ccagggcggc
     4381 agctaccagc aaagccgcgg agcattcctg acatggcttt tgagcgtcac ccacaatatg
     4441 gcgatcgacg agatccggcg tcgcaagcgt cggcccgtcg ccgccgagac gtcggaggac
     4501 gatcaaacgc tcaccatgct gcccgatacc cggacagatg tggagggagc cgcctggcta
     4561 acgacgctgc aggcgatcgt ccgcgatgcg cttcaagaga ttccacagcc gcagcgacaa
     4621 gtgatcgaac tcgcctattt ctccgggctt actcagcgcg aaatttcgga tcggctgaac
     4681 gagccgttgg gcacgatcaa aacgcgcatg cgcctcgggc ttctcaagct tcgcgaccgc
     4741 ctcggaccaa tcctggacga cgatgtctcg gcgccggcca acaaggaaac tgagtgaatc
     4801 gctacgacga tcgaaccccg cacatgacca cacgccaggc aaccgttgac tgcgactggg
     4861 tccgagagca cgcgccagtc catgcactgg gtgccctgga atccgacgtc gctgaccagc
     4921 tcgagagtca cctgctgctc tgccaggagt gcgaccgcga acttgcgtcg ctcatgcgcg
     4981 tgagcgcgtt actcggcgcc ggcgtgaccc aggtcgagcc gccagcgtcg atcaagcgca
     5041 acctgatggc cgcaatcgcg acgccggtcc tcgctgccga gtcgacgact tcaatgctgc
     5101 ccgaaatacc ggctgcggca gcatctccgg ctcccttggc gagtccggcc catgcctgga
     5161 tgtcgtcccg tgcgctcttc gcaccgatcc tgggcgcgct cctgctgctc agcttgtgga
     5221 cggcgcacac gcatcagcag ctcagctccc agcgcgacca agtctcccat ctgcagcgcc
     5281 agaatgacgc gctgacggtt cacctgagct cgcttcaggc cgggcaacag ttcttcggga
     5341 gctcaggctt ctggatgccg ctgagcgtgg tcgatgcgaa cgcaaccgag gcgggcggca
     5401 tcgtcatgag cggcgaccag aatacgacga cgctcctctc tgtctggaac atgccggatg
     5461 accatgacac gtaccacgtg ctttgcgaat ccaagcgcgg cgagctgctg acggccggag
     5521 agatccgggt caacgcgaac ggcagcggta ccgtcacgct caatctcccg tcgcccgtga
     5581 cggaataccg cgcggtccac gtcgtgccga tggaatcggc cacatacgaa gccgatatcc
     5641 tgacacacga catcctgcaa ctgctgatcg gtgagccgac cgccgtcgcg accaacgaga
     5701 cgtagccgcg ccacgttgtc aaacagttcg gccagacact atattccccc ggttagcgat
     5761 tctcgaaatc gcggtctctt cgctcgctcg agcgccattc cgtagggaca aatcagggg
//
LOCUS       DATAOZ010000028         7615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4751821, whole genome shotgun sequence.
ACCESSION   DATAOZ010000028 DATAOZ010000000
VERSION     DATAOZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4751821"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VHR64_00740"
     CDS             <1..202
                     /locus_tag="VHR64_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301380.1"
                     /translation="GFVMTGREIDRGNWPLERAPLSFETSVPGVFAVGDVRQGAVMRV
                     ASAAGEGSVAVGSVHRYLADQT"
     gene            complement(235..3207)
                     /locus_tag="VHR64_00745"
     CDS             complement(235..3207)
                     /locus_tag="VHR64_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3301381.1"
                     /translation="MNSLFGIPMTALMFALLVLLGFCLLTILWVALRRPVIFKLGLRN
                     IPRRKAQTILIVIGLMLSTLIISAALGTGDTLNHSVSAEVYSLIGHVDEAVVYSQSTE
                     GNVNTALNTKIDTSALTLVEQTLAGDPQVDGIMPILLEPVPVVDQSSGQAEPQVTIAG
                     LDPSRIDQFGGLHDRDGHKIDLAALPEGTVVLSKAAAEDLDANVGDTLTIYYGNRPIP
                     LTVAAIAPDSVLSGAVSTEIGGMAMALDRLQQLTNQPNALSIIAISNSGGVRDSLGPT
                     KDVIDRLKPALAGKSLGYQALKQDLVDDTVQFAEIFTDIFLIMGLFSIASGILLIILI
                     FTMLAAERRSEMGIERAVGTQRRQLIQQFVSEGTGYAIFAGIVGAALGVLAAIGIAYG
                     MRLLFGQYFAIEPWVAPRSLVVAYCLGMVLTFAAVVISSWRISRLNITAAVRDIPEVS
                     TPRRKWTILAGGIALLVLGGLMTVAGTSGSQAAPFFLGMSMLPFGGALVIRFLGAPSR
                     PVYSLVGIYLLIFWLMPSNQFNRIFGKYDGGIEMFFLSGICLVFGATLIIVQNTDLLL
                     AAVSKIGRVFRGALPAVRTAIAYPGAARNRTGLTIAMFCLVVFSLVMMATITENFLNL
                     FLGEDANAGWQVRADALSANPIDDFEATLRANGIDTSGFTAMASVTTPHLASQARTLG
                     GDWKDLPVRGMDENFISTSKLKFQQRAEGYATDADIIRALQSHPNLAVIDASAVPASN
                     SFGSDPSLFRLDGLKGSDKVFSPIVIQVADPDNATPASLTIIGVIDSKIGSLYGLYAS
                     RATVDAIFPTTVLTSYYVALAKPDEANAVAKEIEKALLANGVQATSVRDELKDAQRQQ
                     TAFLYIFEGFMGLGLIVGVAAIGVIAFRSVVERRQQIGVLRAIGYQRSLVTLSFMIET
                     GFVVSIGVLTGTALGLMLARNLFNDEDFAASAGGFVIPWTILAVIVVATIASALLMAW
                     VPSRRAARIAPAEALRYE"
     gene            complement(3204..4010)
                     /locus_tag="VHR64_00750"
     CDS             complement(3204..4010)
                     /locus_tag="VHR64_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3301382.1"
                     /translation="MLLEQSQPNGHAVSTPVVEPIIHAAHVHKTYRTGSVKVEALRGI
                     DFTVARGEMVAIMGPSGCGKTTLLNCLSGLDEVDDGQIWLEGRDLATMSDRARTTYRA
                     LRMGFVFQVFNLLPVLSAVENVELPLLVAGIRPGVARKKAIAALARVGLEEWASHRPA
                     ELSGGQRQRVTIARSLVNEPAIVWADEPTGSLDSKTATEIIELLRELNQTAGLTFVLV
                     THDQGVGDRCDRIVQMSDGRIVDEDRPTAIGSLPIIEPAAVAMGDGGYLQ"
     gene            complement(4183..5400)
                     /locus_tag="VHR64_00755"
     CDS             complement(4183..5400)
                     /locus_tag="VHR64_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3301383.1"
                     /translation="MTEVNGPFRELAPARSRIQPRHYFTIAVLLTFVAVLLAARDELG
                     PYILGGLIAYLLVPIARRIEERLPSKGRLASSRHILAVLLTFFLTLAAFLVLLSVLLR
                     PMLDQTSDLLSALPVYWAELQAKYQTFGDWYVANVPEETRAWINAHIDDVGVAIINGA
                     QSIVDFFFNVGGGIFAATITIFTIPLFMIYFLIDQPRLPERIRHQAPTSWGDDAVAVY
                     RIADHILGNYTRGVVLEATIVGVITGTGYWLIGVELALPLGVVAFVGEIVPIAGPWIA
                     FFISFPVVLVTQPDKAIPAVLLFGVIQLLEGWLLTPRIEGSSTDFTSSTTLILLAIGA
                     ALGGAFGMLISLPVAAIARTLIQYTSRRLSGFPADAALEGLLPAYRDRAPAETPIAEP
                     AQAPAATPIETVG"
     gene            complement(5532..5900)
                     /locus_tag="VHR64_00760"
     CDS             complement(5532..5900)
                     /locus_tag="VHR64_00760"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX3301384.1"
                     /translation="MMRRPMARGRRGILGTVATTAVVAGTATAVSNRVTDHQMNKQAA
                     QMNEQYAQQAAYDNQAQMQDMQAQLNQMQAQQVQAAATPAPAEAPDMMSQLQQLAQLK
                     DAGALTEAEFSAAKAKLLGI"
     gene            complement(5921..6349)
                     /locus_tag="VHR64_00765"
     CDS             complement(5921..6349)
                     /locus_tag="VHR64_00765"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEX3301385.1"
                     /translation="MPTGPIEMLAVKFPGNKFTGEIAPALADLIESGTIRVIDILFVR
                     KDADGMVEISEISELDDETYAQFDLVTSDMEGLMSDDDGRRMSELLEPDSSAAVMVFE
                     NTWATRFTEAMRHADGEVVFNERIPRVVIDEMMAEMDANA"
     gene            complement(6396..6611)
                     /locus_tag="VHR64_00770"
     CDS             complement(6396..6611)
                     /locus_tag="VHR64_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301386.1"
                     /translation="MSGLEWIFGVILVTIYIVCLFTVCALTFQKGRTWLGIIGIFFPI
                     LWLIGAFLPPKAGSQFDIAQQTRYRVS"
     gene            complement(6723..7400)
                     /locus_tag="VHR64_00775"
     CDS             complement(6723..7400)
                     /locus_tag="VHR64_00775"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX3301387.1"
                     /translation="MAEAIVSTIALALAVAIDPMRITAIILVLLSDRARRSGPLFLAG
                     YVLSLSVVTAIGLLLSNPVDFGKPDNPSTAATVLLTALSLLILGIASWVWTNRPLPGT
                     EPELPRWLHSVVSLPPILAFGLGLVIAVVSFKNLGLIISAIVLITQANPSVAEEILLF
                     LVFVVVGSLGVGVPVIWYFRAGANAPAMLERWKSWLTLNNAKIVSGSLAFFGLLLLAR
                     SVAHWFD"
BASE COUNT         1436 a         2289 c         2551 g         1339 t
ORIGIN      
        1 cggcttcgtg atgaccggcc gcgagatcga tcgcggcaac tggccgctcg agcgagcgcc
       61 gctctccttc gagacgagtg tgcccggcgt ctttgccgtc ggcgacgttc gccagggcgc
      121 ggtcatgcgc gttgcgtcgg cggccggcga gggatcggtc gcggtcggct cagtccatcg
      181 atatctggca gaccagacct gatttttggc cggcagacgg tggctcaggc gcgtctactc
      241 gtagcgcaac gcctccgccg gggcgatccg tgcggcgcga cgagatggca cccaggccat
      301 cagcagggcc gaggcgatag tggccaccac gatcaccgcg aggatcgtcc acggaatgac
      361 gaagccaccc gccgaggcgg cgaagtcctc atcattgaag aggttgcgag cgagcatgag
      421 cccgagcgct gtccccgtca gcacgccgat cgataccacg aagccggtct cgatcatgaa
      481 gctgagcgtg accagcgagc gttggtagcc gattgcccgc agcacaccga tctgttgccg
      541 gcgctcgacg accgagcgga aggcgattac gccgatcgcg gccacgccga cgatcaagcc
      601 gagccccatg aacccttcga agatgtagag gaaagcggtc tgctgccgct gggcgtcctt
      661 cagttcgtcg cgaaccgagg tcgcctgcac cccgttggcc aacaatgcct tctcgatctc
      721 cttcgcgacg gcgtttgctt cgtccggttt ggccagcgcg acgtagtacg aggtcagcac
      781 ggtggttggg aagattgcgt cgacggttgc ccggctcgca tagagaccgt agaggctgcc
      841 gatcttgcta tcgatgacgc cgatgatcgt caaactcgcg ggtgttgcgt tgtcagggtc
      901 cgccacctgg ataacgatcg gggagaacac cttgtcgctc cccttgaggc cgtccagccg
      961 gaacaggctt gggtcggacc cgaaactgtt gctggccggg acagccgaag cgtcgatcac
     1021 ggcgagattg gggtgggact ggagcgcgcg gatgatgtcc gcgtcggtcg cgtagccctc
     1081 ggctcgttgc tggaacttga gcttgctcgt gctgatgaag ttctcgtcca tgccccggac
     1141 cgggaggtcc ttccagtcgc ctccgagcgt cctcgcctgg gaggcaagat gcggtgtcgt
     1201 cacgctggcc attgcggtaa agccgctcgt gtcgatgccg ttggcccgta atgtcgcctc
     1261 gaaatcgtcg atcggattgg cgctcagggc atcggcccgt acctgccagc ctgcattggc
     1321 atcctcgccg aggaagaggt tcaggaagtt ctcggtgatg gtcgccatca tgacgagcga
     1381 gaagacgaca aggcagaaca tcgcgattgt caggccggtg cgattgcggg ccgcacccgg
     1441 ataagcaatg gccgttcgca cagccggcag cgctccccgg aagacccggc cgatcttcga
     1501 gacggccgcc agcagcaggt ccgtgttctg cacgatgatc aaggtcgcgc cgaagaccag
     1561 gcagatgccc gagaggaaga acatctcgat gccgccgtcg tatttaccga agatccgatt
     1621 gaactggttg ctcggcatta gccagaagat caggaggtag atcccgacaa gcgaatagac
     1681 gggacggctt ggcgcgccga ggaagcgtat gaccagcgcg cccccgaacg gcagcatgct
     1741 catccccagg aagaagggcg cggcctgcga gccggacgtt ccggctaccg tcatgaggcc
     1801 gcccaggaca agaagcgcga ttccccccgc caggattgtc cacttgcgcc ggggcgtgct
     1861 gacttcagga atgtcgcgga cggccgccgt gatgttcagg cgactgattc gccacgacga
     1921 gatcaccacc gccgcgaagg tcaggaccat accgaggcaa taggctacaa ccaggctgcg
     1981 gggcgcgacc cacggctcga tcgcgaagta ctggccgaag agcaggcgca tcccgtaggc
     2041 aatgccgatc gcggccagca cgccgagcgc cgcgccgacg attccggcga agatcgcgta
     2101 tccggttccc tcggagacga attgctggat caattgccgg cgctgggtcc cgacggcgcg
     2161 ctcgatgccc atctccgagc ggcgctcggc ggccagcatc gtgaagatga ggatgatcag
     2221 caggatgccc gacgcgatcg agaagaggcc catgatcagg aagatgtcgg taaagatctc
     2281 ggcgaactga accgtgtcat cgaccaggtc ctgcttgagc gcctggtaac ccaacgactt
     2341 gccggcaagc gccggcttga gacgatcgat gacgtctttg gtcggaccga ggctgtcgcg
     2401 gactccacct gagttcgaga tcgcgatgat gctgagcgcg ttcggctgat tcgtcagctg
     2461 ttggagccga tcgagcgcca ttgccatgcc gccgatttcc gtgctgaccg cgccgctcaa
     2521 caccgaatcc ggggcaattg cggcgacggt cagtggaatg ggtcgattgc cgtaatagat
     2581 cgtcaacgtg tcgccgacat tcgcgtcgag gtcttctgcc gccgccttgc tgaggacgac
     2641 cgttccctcg gggagcgcgg ccagatcgat cttgtgtccg tcgcggtcat ggaggccacc
     2701 gaactggtcg atgcgggacg gatccaggcc ggcgatggtc acctgcggct cggcctggcc
     2761 gctcgattgg tcaacgaccg gcaccggctc gagcaggatc ggcatgatgc cgtccacctg
     2821 cgggtcgccg gcgagagtct gctcgacaag ggtcagcgcg ctggtgtcga ttttggtgtt
     2881 gagcgccgtg ttgacgttgc cttcggtcga ctggctgtag acgaccgctt cgtcgacgtg
     2941 cccgatcagc gaatagacct cggcgctaac agagtggttg agtgtgtcac ccgtaccgag
     3001 cgcggccgag atgatcagcg tgctcagcat cagcccgatc acgatcagga tcgtctgggc
     3061 cttgcggcgg ggaatgttgc gcaggccgag cttgaagatg accggtcggc gcaaggcaac
     3121 ccagagaatc gtgagcaggc agaacccgag cagcacgagt agtgcgaaca tcagcgcggt
     3181 catcgggatg ccgaagaggc tgttcattgc agataccctc catcgcccat ggcaaccgcc
     3241 gccggttcga tgatcgggag ggagccaatc gcagtgggcc gatcttcgtc gacgattcga
     3301 ccgtcgctca tctgcacgat gcggtcgcag cggtcgccga cgccctggtc gtgggtgacg
     3361 agcacgaatg tcaggccggc cgtctggttg agctcgcgaa gcagttcgat gatctcggtg
     3421 gccgtcttcg agtcgagcga cccggtcggc tcatcggccc agacgatcgc cggctcgttg
     3481 acgagtgacc gggcgatggt cacacgctgg cgctggccgc cagacagctc ggccggccgg
     3541 tgcgatgccc attcctcgag tccgacccta gcgagcgcgg cgattgcctt ctttcgggcc
     3601 acacccggtc ggattccggc cacgaggagt ggcaactcga cgttttcgac cgcgctcagc
     3661 accggcaaga ggttgaagac ctggaagacg aatcccatgc ggagggcgcg gtacgtcgtg
     3721 cgcgcgcggt cggacatggt ggccaggtcg cggccttcca gccagatctg accgtcgtcg
     3781 acttcgtcga gcccggaaag gcaattgagc aacgtggtct tgccgcagcc tgaggggccc
     3841 atgatggcca ccatctcgcc acgggccacc gtgaagtcga tgccccggag cgcttcgacc
     3901 ttcaccgagc cggtgcggta ggtcttgtgg acgtgcgccg cgtggatgat cggctcgacc
     3961 acgggtgtgg acacggcatg tccattcggc tgggactgtt cgagaagcat gaccgtggcc
     4021 tccctggttc gcatccggag cgggaccaaa ggagcgcggc cagtgtcccg cacacgtacc
     4081 agtaccgcac gggccgtcca aagggcatgc cgcgcggact ctggagattg caatttcgtg
     4141 acactgcagg aggctggtcc gattgggcgc cgggcttcgg tgctagccga ccgtctcgat
     4201 cggagtggcg gccggcgcct gggcgggttc ggcgatcggt gtctcggccg gtgcgcgatc
     4261 tcgatatgcg ggcagcaacc cctcaagcgc tgcgtccgcc gggaagccgg acagtcgccg
     4321 cgaggtgtac tggatcaggg tgcgggcgat ggcggcgacg gggagcgaga tgagcattcc
     4381 gaaggctccg cccagcgccg ctccgatcgc gaggaggatg agcgtcgtcg aggaggtgaa
     4441 atcggtcgat gatccctcaa ttctcggggt aagcagccaa ccttcgagca actggattac
     4501 gccgaacaag agaaccgccg ggatcgcctt gtcaggttgt gtcaccagca cgaccggaaa
     4561 tgaaatgaag aaggcgatcc acggcccggc gatcgggacg atctcgccca cgaaggcaac
     4621 gacgccaagc ggcaaggcga gttcgacgcc gatcaaccaa tagccggtgc cggtgatgac
     4681 gccgacgata gtcgcctcga gcaccacgcc ccgtgtgtag ttgccgagga tgtgatcggc
     4741 gatgcggtag acggcgacag cgtcatcacc ccatgacgtg ggcgcctggt ggcggattcg
     4801 ctccgggagg cgcggttggt caatcaagaa gtagatcatg aaaagcggga tcgtgaagat
     4861 ggtgatggtg gcggcaaaaa tgccgccacc gacgttgaag aagaagtcga cgatgctctg
     4921 ggcgccgttg atgatggcca cgccgacgtc gtcgatgtgc gcattgatcc aggcccgcgt
     4981 ctcttccggg acgttggcca cgtaccagtc gccgaatgtc tggtatttgg cttgcaactc
     5041 ggcccagtag accggcagcg cactgagcaa atcggacgtc tggtcgagca tcggtcggag
     5101 caggaccgac aggagaacca ggaacgcggc aagcgtcagg aaaaacgtca gaagcactgc
     5161 caggatgtgc ctggagctgg ccaatctgcc tttgctgggc aaacgctcct cgatgcgacg
     5221 agcgattggc acgagcagat aggcgatcaa tccacccagg atgtagggtc ccagttcgtc
     5281 gcgcgccgcc aggagaaccg cgacaaaggt cagcagtacg gcgatcgtaa agtagtgccg
     5341 tggctgaatc cggctccgtg cgggagccag ctccctgaag ggcccattca cctcggtcat
     5401 ctgtgccgct cccttgtcgg ccacggtgta tggcggccgt gcgcggtcaa acgctcaact
     5461 gtgacgtccc cgggagcatc cccgtttccc ggccgagggt ctggcagcga cgaagcagtg
     5521 cgccgtttgg ctcagatccc caggagtttt gccttggccg cgctgaactc ggcctcggtg
     5581 agcgcaccgg catccttgag ctgggcgagc tgctggagct gcgacatcat gtcaggagcc
     5641 tctgccgggg caggcgtggc tgccgcctgg acctgctgcg cttgcatctg gttcagttgc
     5701 gcctgcatgt cctgcatttg cgcctggtta tcgtaggcag cctgctgggc atactgctcg
     5761 ttcatctggg cggcttgctt gttcatttgg tggtcagtga cccggttgga gacggcggtt
     5821 gccgtacccg caacgacggc ggtcgttgct acggttccga ggatccctcg gcgaccccgc
     5881 gccattggtc gtcgcatcat ggctcgctcc tttcaaatgc ctacgcgttt gcgtccatct
     5941 cggccatcat ctcgtcaatg accacgcgcg ggatgcgctc gttgaagacg acttcgccat
     6001 cggcatgtcg catggcttcg gtaaagcgcg tcgcccaggt gttctcgaac accatgaccg
     6061 cggcggacga atcaggttcg agcaactcgg acatgcgccg gccgtcgtca tcgctcatca
     6121 ggccttccat gtcggatgtc acgaggtcaa attgggcgta agtctcgtcg tcgagctcgc
     6181 tgatctcgct gatctcgacc atgccgtcgg cgtccttgcg aacgaagagg atgtcgatca
     6241 cacggatggt tccactctcg atgagatcgg caagcgccgg ggcaatttcc ccggtgaact
     6301 tgttgccggg gaacttgacg gcgagcattt cgatcggtcc ggttggcatg acatgcctcc
     6361 tcaacggtca tccgcgcgtc gcggaacgga attgatcagc tgacgcgata tcgggtctgc
     6421 tgggcaatgt cgaactggga gccggccttc ggcggcagga acgcgccgat gagccaaagg
     6481 atcgggaaga agatgccgat gatgcccagc caggtgcggc ctttttggaa cgtcagggcg
     6541 cagaccgtga acaggcagac aatatagatc gtgaccagga tgacgccgaa gatccactcg
     6601 agaccagaca tggttggttc ctttccgcgg aaagtcgggg cggagttcgg gaacatgatg
     6661 accgagcacg tttggattgc catggcgcgg gggatgcaaa aaggacgcaa gcactgggcg
     6721 cgttagtcga accagtgcgc gacgctccgg gcgagcagga gcagcccaaa gaatgcgagc
     6781 gagcccgaca cgatcttcgc attgttgagg gtcagccaac tcttccagcg ctccagcatt
     6841 gcgggcgcat ttgcgccggc gcggaagtac cagatcaccg ggacacccac acccaggctg
     6901 ccaacgacca cgaagacgag aaagagcagg atttcctcgg ccaccgacgg attcgcctgg
     6961 gtgatcagga cgatcgctga gataatcagg ccgagattct tgaagctcac cacagcgatg
     7021 acaaggccaa gcccgaacgc caggattggc ggcagcgaaa ccaccgaatg cagccagcgt
     7081 ggcagctctg gttccgtgcc gggcaagggc cggttcgtcc agacccacga tgcaatcccg
     7141 aggatcaaga gggagagcgc cgtcaagagc acggtcgcgg ccgtcgaggg attgtcgggc
     7201 ttcccgaaat cgacgggatt cgaaagcagg aggccgatcg cggtcacgac cgagagactg
     7261 aggacgtaac cggccaggaa gagcggcccg gagcgcctgg cccggtccga gagcagcacc
     7321 aggatgattg ccgtgatgcg catcgggtcg atcgccaccg cgagggccaa cgcgatggtg
     7381 gagacgatcg cttcagccat tcatcgtacc cctgtctcgc gcaccggacg tgctggtctc
     7441 aggtcctgga tcgtgcagat gccaccgacc cgcgctgggc gtgcacgggt cggtggcgga
     7501 tggatgaggg gaaattacag ggttaagagg ccgggcttgc ctcgggcgag gctgtaggcg
     7561 tccccggcat gacatcgtcg acgagcggag tgatggtgat cggcttgtct tcgct
//
LOCUS       DATAOZ010000029         7481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1859254, whole genome shotgun sequence.
ACCESSION   DATAOZ010000029 DATAOZ010000000
VERSION     DATAOZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859254"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..2250)
                     /locus_tag="VHR64_00780"
     CDS             complement(199..2250)
                     /locus_tag="VHR64_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX3301388.1"
                     /translation="MPLTIAFRGRLRAGLTALMFLLLALPLSALTPGEVAAAGTYYVS
                     TTGSDATGIGSLAQPWRTIQKAADSVGPGSTVLIQPGTYTGAVEIAIVGTAASAVIFR
                     GNGAGVVIEASSVARDAVLITESSYVTWENIRIQNAPRAGLRVSASNHVTIRHNVFAN
                     NQRWGLFTDFSDDLLIEGNESFGATIEHGIYHSNSGDRPVIRGNVIHDNYANGLHMNG
                     DLSMGGDGTITSPIVEGNYIFNNGLGGGSGINMDGVERAMIRNNVIVDAHASGISMYQ
                     IDGGICSRNNTVYHNTIVVAADGRWAINIPNSGCTGNQLIANILYSNHSYRGAINIVD
                     GARSGFASNYNVLEGVFSTDGGDSILTITQWKALGYDANSRVSTPSALFVNPSSDFHL
                     KPGSPAINVGTTLSSVKTDFDGRARPQGASSDAGAFEAASPADCRLSPTSGPVYTRVT
                     VTCTGFQSGELATVRWDGALVASFTAGSTGAGKVTFSAPRSVTGRHLVKAVGDKGTSV
                     PSRGFVIAPSLSLSPTGGVIGQKITATMRGFGAGEVVTLRFRTAPGSSRTVARNIVAS
                     STGTASASFLVPSGSVGKYSIEAVGSLGGFASAPFTRIFGTAEIPTATPTATASPSPT
                     TTSTPAPPEPTATDEPAVSATPTDEPPAPEPSTEASPNPIQTPNPSQGEAASEIATPA
                     P"
     gene            2395..3408
                     /locus_tag="VHR64_00785"
     CDS             2395..3408
                     /locus_tag="VHR64_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3301389.1"
                     /translation="MVSSLQPPIIDIHGLVKRYDSFTAVDGIDLSVEYGEIYGILGPN
                     GAGKTTTLEMIEGLRMPDSGSITVDGIDAVRESDRVRPIIGVQLQTTALFEYLSAAEL
                     VTLFAGLYESDASPAKVQRLLDMVDLREKAGSQVNTLSGGQRQRLSIALALVNDPRVV
                     FLDEPTTGLDPGARRDLWQMIRGIRADGTTVVLTTHYMEEAEVLCDRVAVMDRGRVIA
                     CDTPAALIASLGIEATVRARITGGSLELVELQSLPAVLSATIEDDRIALQTRDVQTTL
                     IALLRLVEARGVTLAEMTSQQSTLEDVFLSLTGRTYDDTADEPAKEAETVAAGGRRRR
                     RQE"
     gene            3414..4484
                     /locus_tag="VHR64_00790"
     CDS             3414..4484
                     /locus_tag="VHR64_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301390.1"
                     /translation="MRPFLAMVRANLKMTVRNRQALFWNLAFPAIFILIFGAVFSNDN
                     GVTFKVGIAGVDSPYRAAVIQTLEANKSFDVHVGDENKELKALKDSDRDYVLVFGQPA
                     NGQQVAPVEISYDETQGPNAQVGINVLSQVLMGVAGGENAAPITTRPIETLDITYIDF
                     FIPGILAMSIMNAGVIGLSTAFVTYREKGILRRIKVTPFSLTSFILARIATTLIVAVL
                     QAVVLIGLGYLVYGFTMRGNLFLVLITIIIGSLAFLAIGFALSGIARNTETAASYANL
                     VTFPMLFLSGTFFPIDSLPTWLQGLTRILPLRYLIDALRDPMMRGLGIAATWTDLLVL
                     LLVFAMAMVIAVRFFKWDARPA"
     gene            complement(4525..5286)
                     /locus_tag="VHR64_00795"
     CDS             complement(4525..5286)
                     /locus_tag="VHR64_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3301391.1"
                     /translation="MSRGYFVWNDLMTSDPAAVLPFYQGLLGWTTETSDMGTGPYTMF
                     KAGEKHIGGITPGHAGPAYWVSYIMVDDVGAAMKQIESLGGKVLGEPFSVPGVGKMGY
                     AADPNGAVFAPFQDENPDFRPELPSGVQPGGAIAWHEVTTPNQKATDDFYSAIFGWGT
                     TIWPMEHGEYHGLTIGETPVAGVFKAPDGVPAAWTIYFEAPGTIQQAVDDVRRLGGRV
                     LQEIFTVEGTGDITVVADPAGAVFGIMKSLPMGDS"
     gene            5470..5886
                     /locus_tag="VHR64_00800"
     CDS             5470..5886
                     /locus_tag="VHR64_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301392.1"
                     /translation="MEQSIQASKSVNGLVRNYPWLTVLTGMSVLLQAILAGRGWFMDY
                     DLIEVHGVVGNLTFLLVILLVIGAWLGRQAGVMTNTELVLSIALIVLTAAQFGLGYGG
                     RDSRTAASLHIPNAILITGLTSALIAISFIRQPNRA"
     gene            6247..6936
                     /gene="nth"
                     /locus_tag="VHR64_00805"
     CDS             6247..6936
                     /gene="nth"
                     /locus_tag="VHR64_00805"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX3301393.1"
                     /translation="MDVAALPHPPPIDDIHEAMRIVTEAVRDMPKPSMFQLFDEGFST
                     PFEQLIACIISIRTLEETSLPVSRRLFAVARTPDQVAALPVSELEELITPATFPEPKA
                     ANIHAIAVRVRDEFGGALPCDGAVMQEFTGVGPKCANLALGIACGQPTIPVDIHVHRV
                     INRWGYVRAKTPEQTLKALEPKLPMEYRPSINRLLLPFGKYICTGGTPRCLACPLIDI
                     CPKIGVAKARK"
     gene            7153..>7481
                     /locus_tag="VHR64_00810"
     CDS             7153..>7481
                     /locus_tag="VHR64_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301394.1"
                     /translation="MEVAAIRAALMVGSATVVELARRTALTSQAIVRMPYDRIRRQNR
                     RARQPTGTAGHSLASRLGAEDVLSIRESLATGTAVPDELTRQYRVTPRTIRQIATHDT
                     WKHVRGP"
BASE COUNT         1286 a         2316 c         2484 g         1395 t
ORIGIN      
        1 ctgttgtccc agcggcccat ttcaacagtt ggcgtcgact ttgggagcgg tagagacggt
       61 gttgcccgaa gtcaacgact ggactcggtt ttcggcgccg ccgttgccag aatcgacggc
      121 ggcgaatagt gtgctggccg aaatcgatca cgggtttggg ctttcggagc ctgtcgaaca
      181 atctggacct ttcgcgcgtc agggtgccgg tgtcgcgatt tcgcttgctg cttcaccttg
      241 agagggattc ggtgtttgta tcgggttggg ggacgcctcg gtcgatggct caggtgcagg
      301 cggctcatcg gttggagtcg cggaaacagc tggctcgtca gttgctgtgg gttccggtgg
      361 ggcgggggtg ctggtggtgg ttggggaggg tgatgccgtg gcggttggtg tagcagtggg
      421 gatctcagcg gtgccgaaga tgcgggtgaa gggcgccgaa gcgaatccgc ccaggcttcc
      481 gaccgcttcg atgctgtatt tgccgacaga gccgcttggc accaggaacg aggctgacgc
      541 ggtgccggtg ctggaggcga cgatgttccg ggcgaccgtc cgagagctgc cgggcgcggt
      601 ccggaaccgg agcgtgacca cctcgccggc cccaaagccg cgcatcgtgg ccgtgatctt
      661 ctgaccgatc acgccgccgg tcggcgagag cgatagggac ggcgcgatca cgaaaccacg
      721 actgggcacg ctggtgccct tgtcgccgac agccttgacc aggtgccgcc cggtcacgga
      781 gcggggcgca ctgaaggtga ccttgccggc gccggtcgag ccggccgtga acgacgcgac
      841 cagcgcaccg tcccaccgga cggtagccag ctcgccggac tggaagccgg tgcaggtgac
      901 ggtgacccgg gtgtagaccg ggccggaggt cggcgagagt cggcaatcgg ctggggacgc
      961 ggcttcaaat gcaccggcat cggatgacgc accctgcggc cttgccctgc catcgaaatc
     1021 ggtcttgact gagctcaggg tggtgccgac gttgatcgcg ggcgagccgg gtttcaggtg
     1081 gaagtcgctg ctcgggttca cgaagagcgc ggatggcgtt gatactctcg agttggcgtc
     1141 gtagccgagt gccttccatt gcgtaatggt caggatcgag tcgccgccgt cggtgctgaa
     1201 gacgccctcg aggacgttgt agttgctggc gaagccggaa cgcgcgccgt cgacgatgtt
     1261 gatcgcgccc cgatagctgt ggttgctgta gaggatgttg gcgatcagct ggttgccggt
     1321 gcagccgctg ttcgggatgt tgatggccca gcgaccgtcc gccgcgacca cgatcgtgtt
     1381 gtggtaaacg gtgttgttgc gggagcagat gccgccgtcg atctggtaca tcgagatgcc
     1441 actggcgtgc gcatcgacga tcacgttgtt gcggatcatc gcccgctcga cgccgtccat
     1501 gttgatcccg gaaccgccgc ccaatccgtt gttgaatatg tagttgccct cgacaatagg
     1561 gctggtgatt gtgccgtcgc cgcccatcga gaggtcgccg ttcatgtgca ggccgttggc
     1621 gtagttatcg tggatcacgt tgccacggat aaccggccgg tcgccgctgt tcgagtggta
     1681 tatgccgtgc tcgatggtcg cgccgaatga ctcgttgccc tcgatcagaa gatcgtcgct
     1741 gaagtcggta aagagacccc agcgctggtt gttggcgaac acgttgtgcc ggatggtgac
     1801 gtggttagag gccgagacgc gcagtccggc ccggggcgcg ttctggatcc ggatgttctc
     1861 ccacgtgaca taactggatt cggtgatgag aaccgcgtcg cgcgcgaccg aggatgcctc
     1921 gatcaccacg cccgcgccgt tgccgcggaa gatgaccgcg ctggcggctg ttccgacgat
     1981 cgcaatctcg accgcgccgg tgtaggtgcc gggctggatc aggaccgtcg agccgggacc
     2041 gactgagtcg gccgccttct ggatcgtccg ccacggttgg gcgagcgagc cgatgccggt
     2101 tgcatcgctt ccggtggtcg agacgtaata cgtaccggcc gcggccacct cgcccggagt
     2161 cagggcggac aggggcaagg ccagcaggag gaacatcagc gcggtcaggc cggcgcggag
     2221 cctcccccgg aatgcaatcg tcaacggcac ggaacgcctc cagccggagc gaaacggtac
     2281 ccggcagtgc agttctaccg gaacccggcg gtcgaataac aggccgcggg tgcctcggga
     2341 gtcctgtcca ggaggcgatc ggtagtagca ttgagcggct ataacaggag acagatggtc
     2401 agctcactcc aacccccaat tatcgacatc cacggactcg tcaagcgcta cgactccttc
     2461 accgccgttg acgggatcga cctctcggtc gagtacggcg agatctacgg aattcttggc
     2521 ccgaacggcg ccggcaaaac cacaacgctc gagatgatcg agggtctgcg catgcccgat
     2581 tccggctcga ttaccgtcga cgggatcgat gccgtgcgcg agagtgatcg cgtccggccg
     2641 atcattggtg tccagcttca gaccacggcc ctgttcgaat acctgagcgc ggccgagcta
     2701 gtcacgctgt ttgccgggct ctacgagagc gacgcgtcgc cggccaaggt gcagcggctg
     2761 ctggacatgg tcgacctgcg ggagaaggcc gggtcgcagg tcaatacgct ctccggcggg
     2821 caacggcagc gcctgtcgat cgcgctggcg ctggtcaacg atcccagggt ggtcttcctc
     2881 gacgagccga cgaccgggct tgacccgggc gcgcggcgag acctctggca gatgatccgc
     2941 ggcatccgcg ccgacggcac gacggtggtg ctgaccacgc attacatgga agaggccgag
     3001 gtgctctgcg atcgggtcgc ggtcatggat cgtggccggg taatcgcttg cgatacgccg
     3061 gccgcgctta tcgcgtcgct cgggatcgag gcgacggtgc gggcccggat cactggcggc
     3121 tctctggagc tggtcgaatt gcagtcgctt ccggcagtgc tgagcgcgac aatcgaggac
     3181 gatcggattg ccctgcagac gcgcgatgtg caaacgacgc tgatcgcact gctccggctg
     3241 gtcgaggcgc gcggcgtgac gctggccgag atgaccagcc agcaatcgac gctcgaggat
     3301 gtcttcctca gcctgaccgg acgaacctac gacgacactg ccgatgagcc ggccaaagag
     3361 gcggagaccg tcgcggccgg tgggcggcgt cgccggcgcc aggaatgaat ctgatgcgac
     3421 cgtttcttgc catggtcagg gccaacctga agatgacggt ccggaaccgc caggcgctgt
     3481 tctggaacct cgcgtttccg gcgatcttca tccttatctt cggagcggtg ttcagcaatg
     3541 acaacggcgt cacgttcaag gtcggcatcg ccggcgtcga ctcgccgtat cgggcggcgg
     3601 tcattcagac gctcgaagcg aacaagtcct tcgatgttca tgtcggcgat gagaacaagg
     3661 agctgaaggc gctcaaggac agcgaccgcg actacgtgct ggtcttcggg cagcccgcca
     3721 acggccaaca ggtcgcgccg gttgaaatct cgtatgacga gacgcagggt ccgaatgcgc
     3781 aggtcgggat caacgtcttg tcgcaggtgc tgatgggcgt ggcgggtggc gaaaatgcgg
     3841 cgccgattac gacgcggccg atcgagacgc tggacatcac ctacatcgac ttcttcatcc
     3901 ccggtattct ggccatgtcg atcatgaacg ccggagtcat cgggctttcg acagccttcg
     3961 tcacctaccg ggaaaagggc atcctgcgcc ggatcaaggt gacgcccttc tcgctgacca
     4021 gcttcatcct ggcccgaatc gcgacgaccc tgatcgtcgc cgtattgcag gcggtcgtgc
     4081 tgatcggcct cggctacctc gtctacgggt tcacgatgcg tggcaacctg ttcctggtcc
     4141 tgatcacgat catcatcggc tcacttgcct ttctcgcgat cggtttcgcc ctttccggaa
     4201 tcgcccggaa taccgaaacc gctgccagtt acgcgaacct cgtgacattc ccgatgctct
     4261 tcctctccgg cacgttcttt ccgatcgatt cgctgccgac ctggctgcag ggcctgacgc
     4321 gaatcctgcc tctgcgatac ctgatcgacg cactgcggga tccgatgatg cgcgggcttg
     4381 gaattgccgc gacgtggacg gatctgctgg tcctgctgtt ggtgttcgcc atggcgatgg
     4441 tgatcgccgt ccgcttcttc aagtgggatg cgcgtccggc atagggaccg ggggcggccg
     4501 cgtccggccg cccggcgatc ccgtctagct gtcacccatc gggagcgact tcatgatccc
     4561 gaagaccgcg ccggctggat cggccacaac ggtgatgtcc ccggtgcctt cgaccgtgaa
     4621 gatttcctgc agcactcggc caccgagccg gcggacatcg tccaccgctt gctggatcgt
     4681 gcccggcgcc tcgaagtaga tcgtccaggc agccggcacg ccatcgggcg ccttgaagac
     4741 accggcaacc ggggtctcgc cgatggtcag accgtggtac tcgccgtgct ccatcggcca
     4801 gatcgtggtg ccccagccga agatggcgga gtagaagtcg tccgtcgcct tttgattcgg
     4861 cgtcgtgacc tcgtgccagg cgatcgctcc gcccggctgg acgccgctcg gcagctcagg
     4921 ccggaaatcg ggattctcgt cctggaatgg ggcgaagaca gcgccgttgg gatctgccgc
     4981 gtagcccatc ttgccgacgc ccgggacgct gaagggctcg ccgaggacct tgccgccgag
     5041 cgactcgatc tgcttcattg cggcgccaac gtcgtcaacc atgatgtagg agacccagta
     5101 ggcgggtccc gcgtggccgg gggtgatgcc gccgatgtgc ttttcgcccg ccttgaacat
     5161 cgtgtacggg ccggtgccca tgtccgacgt ttcggtagtc cagcccagca gaccttggta
     5221 gaagggcagc acggcagccg gatcggatgt catcaggtcg ttccagacga aatagcctcg
     5281 gctcacagag attcctttca acggagattg aatggacgag cggggttggc gatgaggact
     5341 gcacaatagc acagatgttc gatctgtcaa ggtgcttcac cggttcttca cagtcgtgcc
     5401 attctggccg aatccgacgt ggcagacttc tcacggattc tgaatttggc gacgatcagg
     5461 agtcattgcg tggagcagag tattcaagcc agcaaatcgg tgaatggatt ggtccggaac
     5521 tatccgtggc tgacggtgct gaccgggatg tcggtcctgc tccaggcgat cctggccggg
     5581 cggggctggt tcatggacta cgacctgatc gaggtgcacg gcgtcgtcgg caacctgacc
     5641 ttcctgctcg tgatcctgct cgtgatcggc gcctggctcg gccggcaggc gggcgtgatg
     5701 accaacaccg agctggtcct cagcatcgcg ctgatcgtgc tgaccgcagc ccagttcggc
     5761 ctcggctacg gcggtcggga cagccgaacc gccgcgtccc tgcacatccc gaacgcgatc
     5821 ctgattaccg gcttgacctc ggccctgatc gcgatcagct tcatccgcca accaaaccgg
     5881 gcgtagcacc ggcgatttcc atcctgagcg tggttgtcca gcgccgcccg gggataaatg
     5941 cccgggctga acaacgggaa gcccactaaa ggtgctgaaa atacgtcgct tcgggatgcc
     6001 gtctgctcgt agccccgtta gtgggcttct cattcctcag cccgaacctt tagcttcggg
     6061 cggcgtggga tgtcggcttc ctcatcggga ccggctcatc tccaatggat gcacgatgcg
     6121 tcgcccgcgc actctctttg tcgaagcgaa cctttgcgcg acggcgtagc acccgcgatt
     6181 ccccgggttt gcctccgccg gtagcatgaa tcccgtcgtt cgcacggatt ggcggagggg
     6241 gatcgcatgg atgttgccgc gttgccgcat ccgccgccga tcgacgacat ccacgaggca
     6301 atgcgcatcg tcactgaggc ggtgcgggac atgcccaagc cctcgatgtt ccaactcttc
     6361 gacgagggct tcagcacgcc gttcgagcaa ctgatagcct gcatcatctc gatccgcacg
     6421 ctcgaggaga cgagcctgcc ggtctcgcga cgcctgttcg cggtcgcgcg aacacccgat
     6481 caggtcgccg ccttgccggt ctctgagctg gaggagctga tcacgccggc gaccttccca
     6541 gagccgaaag cggccaacat ccacgcgatc gctgtccgcg tgcgggatga gttcggcggc
     6601 gcgctgccct gcgacggcgc ggtgatgcag gagttcaccg gcgtcggccc gaagtgcgcc
     6661 aacctcgccc tcggcatcgc ctgcgggcag ccaacgatcc cggtcgatat ccacgtccac
     6721 cgggtcatca accgctgggg gtatgtccga gccaagacac cggaacagac gctgaaggca
     6781 ctcgaaccga agctgccgat ggagtatcgc ccctcaatca accgcctgct gctgcccttc
     6841 gggaagtaca tctgcaccgg cggcacgccc cgctgtctcg cctgccccct gatcgacatc
     6901 tgccccaaga tcggcgtcgc caaggcgagg aaatgagtcg atgccacggt tgacagcacg
     6961 tcgaggtcag gcgcgaaacc ccacttgaag ccagaaaccg ggccggccct cttgcgcggc
     7021 gtcatcagtt ggtcttgaat gcaagggtat tgagcgcaaa cgggcggcac cgtgccgtcg
     7081 cacgaatgtg ttccgtcgtt ctccgcgaag tggagtgtcg ctatgcaacc cttgcaggtg
     7141 aggatgagtg ccatggaggt ggccgcgatt cgggccgccc tgatggtggg gtcagcgaca
     7201 gtggttgagt tggcccgccg gaccgcgctc acgagtcagg cgatcgtccg gatgccgtat
     7261 gaccggatca ggcgtcagaa ccgcagagca cgccagccaa caggtaccgc cggccactca
     7321 ctggcgagtc gcctcggggc ggaagacgtg ctctcgatcc gggaatcgct ggcgaccgga
     7381 acggcggttc ccgacgagct gacgcggcag taccgggtga ctccccgcac catcaggcaa
     7441 attgcgaccc acgacacctg gaagcatgtc cgcggcccga a
//
LOCUS       DATAOZ010000030         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_17891, whole genome shotgun sequence.
ACCESSION   DATAOZ010000030 DATAOZ010000000
VERSION     DATAOZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17891"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VHR64_00815"
     CDS             <1..288
                     /locus_tag="VHR64_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301395.1"
                     /translation="QVPAAHQFIDFMLSPNEQAALANRTTRAIVNLAAVPMIDPAISA
                     FTNYADLDAVIAESPPLGFPPLEESADGTASYLDWVLSWEKVRSLKSNAAP"
     gene            378..1358
                     /locus_tag="VHR64_00820"
     CDS             378..1358
                     /locus_tag="VHR64_00820"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX3301396.1"
                     /translation="MALDTGRVVDVVKQTDEAPHGGEGWVARETTMPQELGSLWAECG
                     VTSEYGELKSVLMRTPGPEIEAVTDPRPALWYDLLDPVRAREQHAALVDLYRSHGVTV
                     NLGQPVPLDKPNAYFCRDHFCMTPTGAVIARMASASRAGEERYAAADLAALGVPIIHS
                     VFGDATFEGADVVVASPDLVFVGHGMRSNRAGAEQVAGAFRRIGVPEVIVVEIPYGCG
                     HIDGTINVVDRDLALVFPTQLSWVVYETMRRHGFRFLDLPSGEEAQMGMAINMVPLAP
                     GVIVLPAGNPVTKATLEANGVECLEVEISELMKGGGAVHCMTGVIHRAPA"
     gene            1374..>1726
                     /locus_tag="VHR64_00825"
     CDS             1374..>1726
                     /locus_tag="VHR64_00825"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEX3301397.1"
                     /translation="MALASHHCTFCISNWNEETTTEFPDGSKLTRASVSQAYDGDMAG
                     TSTVEYLMTYLPSGSVKFIGFEVLTGSIGGRSGTVVMQHDGLFVGGRARSTWSFVEDS
                     GTGELINLHGNGSYES"
BASE COUNT          284 a          550 c          599 g          293 t
ORIGIN      
        1 caggtcccgg ccgcacacca gttcatcgac ttcatgctct cgccaaacga gcaagccgcg
       61 ctagccaatc gcacgacgcg ggcgatcgtc aatctcgcgg cggtgccgat gatcgacccg
      121 gccatcagcg ccttcaccaa ctacgccgat ctcgacgccg tcatcgcgga atcgccgccg
      181 ctcggctttc cgccgctcga ggaatcggcc gacggcaccg cctcctacct cgactgggtc
      241 ctgtcgtggg agaaggtgcg ttcgctgaag agcaatgccg ctccgtgagt cgtcagactt
      301 cggacttcag atgtcagttg gttgggatag actgcgcgtc acactgaatt ccgattactc
      361 atgtggagcg agatgccatg gcgctggata cggggcgcgt tgtcgatgtt gtcaaacaga
      421 cggatgaggc accgcacggg ggcgagggct gggtcgcgcg cgagacgacc atgccgcagg
      481 agcttggctc gctctgggcc gaatgcggcg tcacctccga gtacggcgag ctgaaatcgg
      541 tcctgatgcg gacgcctggc ccggagatcg aggcggtgac cgatccgcgc ccggcgctct
      601 ggtacgacct gctcgatccg gtccgggcgc gggagcagca cgctgcgctg gtcgacctct
      661 atcggtcgca cggggtaacg gtcaatctcg gccagccggt cccgctcgac aaaccgaacg
      721 cctacttctg ccgcgaccac ttctgcatga ccccgaccgg cgcggtgatc gcgcggatgg
      781 cctcggccag ccgggccggg gaggagcggt acgcggcggc tgatctggcc gcgctcgggg
      841 tgccgatcat ccactccgtg ttcggcgacg ccaccttcga gggcgccgat gtcgtggtgg
      901 cgagcccgga tctcgtcttc gtcggccacg ggatgcgcag caaccgggcc ggggccgagc
      961 aggtggccgg ggcgttccgg cggatcggcg tgccggaggt gatcgtcgtg gagatcccgt
     1021 acggttgcgg ccacatcgac ggcacgatca acgtggtcga ccgcgatctg gcgctggtct
     1081 tcccgacgca gctctcgtgg gtcgtctacg agacgatgcg gcggcacggc ttccgcttcc
     1141 tcgatctgcc gagcggcgag gaggcgcaga tgggcatggc gatcaacatg gtcccgctcg
     1201 cgccgggcgt gatcgtgctg cctgccggta atccggtcac gaaggcgaca ctcgaggcga
     1261 acggggtcga gtgcctcgag gttgagatca gcgagctgat gaagggcggc ggcgcggtcc
     1321 actgcatgac cggcgtcatc caccgcgccc cggcctgaga aaaggaggtc cgcatggcgc
     1381 ttgcctcgca ccattgcacc ttttgcattt cgaactggaa cgaggagacg acgactgagt
     1441 ttccggacgg ctcgaagctg acccgggcgt cggtctcgca ggcatatgac ggcgacatgg
     1501 ccgggacgag cacggtcgag tacctgatga cgtacctgcc gtccgggtcc gtcaagttca
     1561 tcggcttcga ggtgctgacc ggctcgatcg gcgggcgatc ggggacggtc gtgatgcagc
     1621 acgacggcct cttcgtcggt ggcagggctc ggagcacatg gagcttcgtc gaggactccg
     1681 ggaccggcga actgatcaat ctccacggca acggcagcta cgaatc
//
LOCUS       DATAOZ010000031         8540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5279035, whole genome shotgun sequence.
ACCESSION   DATAOZ010000031 DATAOZ010000000
VERSION     DATAOZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5279035"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="VHR64_00830"
     CDS             <1..1361
                     /locus_tag="VHR64_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301398.1"
                     /translation="TDRFLIVDEGAPHGFALGLGWLVDVSADPDPETFGDVLIRYRAD
                     DANVLFRLRPGKSRLALRARGKPEELVAALSDAGAEVADLARDVADTLRLSWDSAASL
                     ETESVVWRGQATAPLRPGLECAPASVWITPTALVWGSQKIRGVNRLPISTISRLSSTT
                     LGDEHGSPTVYVRSRVISDVRLDLPFIFNLGAVRENVTARGEFLGLFRPDSVIEGSVS
                     ARPQPWTEDAPAESDDEVEIIVGPDAEAVADDMPESDEREPAEDETPTFETWANVTKP
                     AAFPYVPPAEGHTTIHLHGDQEEEFESASTRLVDAIASWPGTASIPAEPESAAVPVVT
                     EPDRIPAYLKVARRAIDEVNEAIDRRTSGNAAPPLRATPPSANEQAEALSELIELTGS
                     DYYSAEQARALKARITRTGEAAVRLRSLIELCNAGHMTIHEVGIKRDAIMANLPAAVE
                     DE"
     gene            1547..3901
                     /locus_tag="VHR64_00835"
     CDS             1547..3901
                     /locus_tag="VHR64_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX3301399.1"
                     /translation="MLLGSVRASRKKPSRMPQFVIAGVILLTLTFLIAVVSFVVGSVT
                     AVSATVHQYREVNAQLPNAADVASNAFQTTTIYDRNGVLLQQVDQKDGGWRNFVPLED
                     VSPYLIQATISAEDATFWTHYGVEPIAIVRGATIILGGEGSSGGSTITQQLARGLYPN
                     QIGTDYSLTRKAHEAMAAVALEKEFSKDDILTMYLNLIFYGQRSYGIEAASNTYFQKH
                     ASELDLAEASMLAGLPQAPSQYDPTWNFDLAKKRQKYVLDQMVKYDYITQDQADKAFE
                     EKLDPQTRDGSIRPGAEHFTYFVQNYIEDHWPGALYNGGLKFTTTLDVDLQATAQNIV
                     TSTVQNLLLGYQRNNAAMVVMVPWSGEVLAMVGSADYNNEFIGGQNNYAWERRQPGSS
                     IKPLVYAAAFESGWTPGTVVMDVPYTEKVDNPSNPSDAVWKPQNYNTFNYGAVSVRTA
                     LANSLNIPAVKAIKHAGIQHAMATAQKMGLVTSLNRPWYDYGNAFALGSGEVRLIEHT
                     NAYATIANNGKFVPYNPILKIEDSQGHVLYDATKTNPLDTANQAIRAGYAYQLTSILT
                     DNDARSMIFSQNNLFGNTQTQLGRPTAAKSGTTDGWKDIWTMGYTTDVAIGVWVGNTS
                     ADGTSPQQLPEFDGIQGAGPIWQKMMYEVHGPQWSALLNGPDGNPLPENFPRPSEVYD
                     GNVCASTGNQATPGFESKKEVLVRGGGPYLACDQLSAEQSAELTAATDALRTGAKWTN
                     GAQDKIRRYQSAVSGNGDSITEYDSNNNSDNFNNDDEQPIEPLN"
     gene            4253..4963
                     /locus_tag="VHR64_00840"
     CDS             4253..4963
                     /locus_tag="VHR64_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301400.1"
                     /translation="MRTARNLPIFLGLSAGIYAACIRPRLLHWGATQDEVQRPFPGAD
                     LIPGGTRSATMATTIDAPPDHVWPWLVQMGTDRAGWYSWDHLDNWGHSSETRIHPEWQ
                     EIKVGDRMAAMSDGDVAWEVAALEPERFLGLRMSLDLRGRPFDPTEVPPRFYTDSLWA
                     FLLEELPGNRTRLIVSGYWAFQPRWLQPLMTVLFLDLQHWIMQMRQFANLKHRVEQAK
                     PATRYPSPMVDLAAARIR"
     gene            4986..5669
                     /locus_tag="VHR64_00845"
     CDS             4986..5669
                     /locus_tag="VHR64_00845"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX3301401.1"
                     /translation="MINKILLGCGAVSSALYIISDVIAARRYDGYSYSDQWFSELTAE
                     GAPTRPLMTVTNEIPYTALMTAFAMGIWKTAGQNRAQRLTAALLGGYAAAGIAGGVGF
                     PMATRDAEASLRNIMHIPSTMVMSLCILAAMGTASRLFGNRFRYYSYSTIAAAITFGV
                     LTSSQAGKISKNQPTPWAGIEERVNIYATMLWLAMLSIGLLRALRPTDSRTAEKPAVT
                     ALPMHGLPQ"
     gene            5842..6060
                     /locus_tag="VHR64_00850"
     CDS             5842..6060
                     /locus_tag="VHR64_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301402.1"
                     /translation="MRLELVDQEDNQRTIGAIVVTQDGGEISIDAGPADGLPLVRLKI
                     SRQEALKLSANLRAVANGRDEEVLLDDQ"
     gene            complement(6464..7210)
                     /locus_tag="VHR64_00855"
     CDS             complement(6464..7210)
                     /locus_tag="VHR64_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3301403.1"
                     /translation="MLKRRIMIVDDHPMFRIGLRRLLESDPRLAVVAEAENGHQALHQ
                     ADIHKPSVVLMDVQLPGVTGIHVASALRRERPTTSVIFLSMHVDDDRLFSAVRVGAAA
                     FLTKDTEPTRILETVHSVLKGENLLHQAVLTNPELARRVLGEFRTLSDGNGVPAAEVA
                     LSPRELEVLDCLVMGHSNKEIGDALFITEQTVKNHMTSVLRKLQVDDRVAALRYAVTR
                     RWAEIGPQPYAEVEQAKTPQPVGESKSSAA"
     gene            7496..>8540
                     /locus_tag="VHR64_00860"
     CDS             7496..>8540
                     /locus_tag="VHR64_00860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX3301404.1"
                     /translation="MTPRRVVVTGVGAVTSLGPTAKQTWEKLLACESGLKRIDLDPEQ
                     NPCLVRGDVDDSTISNRFLDGKALRNTSRFSRMAVEAAGESLIDAGLIGEDGTPVADL
                     SDAGTVIGTCVGGAHDDLLPAWTTYENRGPGRVPPHLHVMFPLNLASYTIQWRWGMQG
                     PTNTVNTACATGSQAIGEAFHQVKYGKADIMVAGAVESDSHPMFIAGFAAMKALATDS
                     NDNPPAASRPFDQSRAGFVLGEGAGMVVLEEYEHAKARGAKIYAEVLGYASTNDAFHP
                     IAPRPDGSGSAKAVRNALKDAGVEPDEVGHVQAHAASTPVGDVAEANAIKEVFGDRAT
                     SIPVTSIKGAIGHC"
BASE COUNT         1633 a         2708 c         2736 g         1463 t
ORIGIN      
        1 tcaccgaccg cttcctgatc gtcgacgagg gcgcgccgca cggcttcgcg ctcgggctcg
       61 gctggttggt cgatgtctcg gccgaccccg atccagagac cttcggcgat gtgctgatcc
      121 ggtaccgagc ggacgacgcg aatgtccttt tccgtctgcg gccgggcaaa tcgcggctgg
      181 cgctgcgggc acgcggcaag ccggaggaat tggtcgccgc attgagcgat gccggtgccg
      241 aggttgcgga tctggcccgg gatgtggccg acacgttgcg gctgtcgtgg gactcggcgg
      301 cgagtctcga gaccgagtcg gtcgtctggc gcggtcaggc gacggctccc ctgcggccgg
      361 gccttgagtg cgcaccggcg agcgtctgga tcacgccgac cgcgctggtc tggggcagcc
      421 agaagatccg gggcgtcaat cggctcccga tcagcacgat cagccggctg tcgtcgacca
      481 ccctcggcga cgagcacgga tcgccgaccg tctacgttcg ctcgcgggtg atctcggacg
      541 tgcggctgga tctgccattc atattcaacc tcggagcggt ccgggaaaac gtgacagcgc
      601 ggggcgaatt cctcgggctg ttccgacccg actccgtcat tgagggctcg gtctccgccc
      661 gaccgcaacc ctggacggag gacgcgccgg cggagtcaga cgacgaggtc gagataatag
      721 tcggcccgga tgctgaggcg gtcgcggacg atatgcccga atcagatgag cgtgaaccgg
      781 cggaggatga gacgccgaca ttcgagacgt gggcaaacgt cacgaagccg gcggcatttc
      841 cgtatgttcc gcccgcggag gggcatacca ccatccactt gcacggcgat caggaggagg
      901 aattcgaatc ggcgagcacg cgactggtcg acgcgatcgc gtcctggccc ggtactgctt
      961 cgatcccggc ggaaccggag tctgccgccg tgccagtcgt aaccgagccg gatcgcatcc
     1021 cggcgtacct gaaggtggcc cgccgcgcga tcgacgaggt caatgaggca atcgaccggc
     1081 gtacgtctgg caacgccgcg ccgccgttgc gggcgacgcc gccatccgcg aacgagcagg
     1141 ccgaggcgct ctcggaactg atcgaactga ccggttcgga ctactattcc gcagagcagg
     1201 cccgcgcgtt gaaggcgcgc atcacccgca ccggcgaagc cgccgtgcgc ctgcgctcat
     1261 tgatcgagct gtgcaacgcc ggccacatga ccattcacga ggtcggcatc aagcgcgacg
     1321 cgatcatggc gaatctcccc gctgccgttg aggacgagtg aaaccgcccg cgctgttcga
     1381 ccgttgggaa tgacgtaccg ccgcgacccg cgccgcgccg acacgatcgg cggagccgga
     1441 caccgggagg ctgatggacc aggaactcat ccgcgcacga atccgacacg ctgagcgtcg
     1501 ccggcgtaag gttcaaggca atcgccgcaa gcgatccttg ccgccaatgc tgcttggcag
     1561 cgtccgcgca tcccgcaaga agccctcgcg gatgccgcag ttcgtgatcg ccggagtcat
     1621 cctgctgacg ctgaccttcc tgatcgcggt cgtcagcttc gtcgtgggct cggtgacagc
     1681 ggtcagcgcg acggtgcatc agtaccgcga agtgaacgcg cagcttccga acgcagccga
     1741 cgtcgcctcg aatgcctttc agacgaccac gatctacgat cgcaacggcg ttctgcttca
     1801 gcaggtcgac cagaaggatg gcggctggcg gaactttgtt ccgctcgagg atgtctcgcc
     1861 gtacctgatc caggccacca tttcggccga agacgcgacc ttctggaccc attacggcgt
     1921 cgagccgatc gcgattgtcc gcggcgcgac gatcatcctc ggcggcgagg gatcgtccgg
     1981 tggttcgaca attacccagc agcttgcccg gggtctctat ccgaaccaga tcggcaccga
     2041 ctactcgcta acccgcaaag cccacgaggc aatggcggcg gtcgcgctcg agaaggagtt
     2101 ctcgaaagac gacatcctga cgatgtacct caacctgatc ttctacggtc aacgctcgta
     2161 cgggattgag gccgcctcca acacctactt ccagaagcac gccagcgagc tggatctggc
     2221 cgaggcttcg atgctggccg ggctgccgca ggcgccatcc caatacgacc cgacctggaa
     2281 cttcgacctg gccaagaagc ggcagaagta cgtgctcgac cagatggtca agtacgacta
     2341 catcacgcag gatcaggccg acaaagcctt cgaggagaaa ctggacccgc aaacccggga
     2401 cggctcgatt cggccaggcg ccgagcattt cacctatttc gtgcagaact acatcgagga
     2461 tcactggccg ggcgcgctct ataacggcgg cctcaaattc acgaccacgc tcgacgtcga
     2521 tttgcaggca acggcgcaga acatcgtgac cagcacggtc cagaacctgc tgctcgggta
     2581 tcaacgcaac aacgcggcca tggtcgtgat ggtgccctgg agtggcgaag tgctggccat
     2641 ggtcggctcg gccgattaca acaacgagtt catcggcggg cagaacaact atgcctggga
     2701 acgcaggcaa ccaggctcat caatcaagcc gctggtttac gcggccgcat tcgagagcgg
     2761 atggacgccg ggcaccgtcg tgatggatgt gccgtatacc gagaaggtgg acaacccgtc
     2821 caacccgagt gatgcagtct ggaagccgca gaactacaac acgttcaact acggagcggt
     2881 ctcggtccgg accgcgctgg ccaactcgct caatatcccg gcggtcaagg cgatcaaaca
     2941 tgccgggatc cagcacgcga tggcgaccgc gcagaagatg ggattggtca cgagtctgaa
     3001 tcgtccgtgg tacgactacg gcaacgcctt tgccctcggg tcgggcgaag tgcgactgat
     3061 cgagcacacc aatgcctacg cgaccattgc caacaacggc aagttcgtgc cctacaaccc
     3121 gattctgaag atcgaggact cacagggtca cgtactctac gatgcgacca agacgaaccc
     3181 gctcgacacg gccaaccagg cgatccgggc cgggtacgcc tatcagctca cgtcgatcct
     3241 gacggacaac gatgcccgct cgatgatctt cagtcagaac aacctgttcg gcaacacgca
     3301 gacccagctc ggccgcccga ccgcggccaa gtccggcacg actgacggct ggaaagacat
     3361 ctggacgatg ggctacacga ctgacgtcgc gattggcgtc tgggtcggaa acaccagcgc
     3421 agatggaaca tcacctcagc agctgccgga gttcgacggc atccagggcg caggcccgat
     3481 ctggcaaaag atgatgtacg aagtacacgg tccgcagtgg tccgcgctcc tgaacgggcc
     3541 ggatgggaat ccgctgccgg agaacttccc gcgtccgagt gaggtctacg atgggaacgt
     3601 atgtgcctcg accggtaacc aggcgacgcc gggcttcgag agcaagaagg aggtcctcgt
     3661 tcgcggcggt gggccttatc tggcctgcga tcagctctcg gctgagcaat cggcggagct
     3721 gacggcggcc accgatgcgc tccgcacggg tgcgaagtgg actaacggcg cccaggacaa
     3781 gatccgccgt taccagagcg cagtcagtgg caatggcgac tcgatcaccg aatacgactc
     3841 gaacaacaac tcggacaact tcaacaacga cgacgagcag ccgatcgagc cgctcaatta
     3901 agccatctcc agagcggtag tccttccttc gtagcgcggg gttaccacgc ccagcatcga
     3961 tgatctccac gcacgggaga tgcagccgga ttacaaacgg ggttggttcc ccttacgcat
     4021 gtggcgtcat cgggtccggg cgaggcgtgg taaccccgcg ctacgtcgag cttccgttcc
     4081 gttgtccggt gtttttcatt gacatgaagg tgcaatggtc accgcgcgtt agcggtcgcg
     4141 ggtgtgtcgg gcgtgcgcat agcgtgagtt cgggaagcga ggtttgcgcc tggaggcggt
     4201 cccggaacct gggggcgttc gctcatcggc atgcgaaagg agcgatgctg agatgcgcac
     4261 tgcacgcaac ctcccgatct ttctcggtct ttccgcggga atctacgcag cctgcatccg
     4321 acctcgatta cttcactggg gcgccaccca agacgaggtc cagcggccat tcccaggcgc
     4381 cgacctcatc cccggcggca cccgcagcgc gacgatggcg accacgatcg atgcgccacc
     4441 cgaccacgtc tggccgtggc tggtgcagat gggtaccgac cgcgccggct ggtatagctg
     4501 ggaccatctc gacaactggg gacattccag cgagacacgg atccaccccg agtggcagga
     4561 aatcaaagtc ggcgaccgta tggccgcaat gtcggacggc gacgttgcct gggaggtggc
     4621 ggcgctcgag ccggagcgat tcctcgggct gcgcatgtcg ctcgatctgc gcggccgccc
     4681 gtttgaccca acggaagtgc caccccggtt ctataccgac tcgctctggg ccttcttgct
     4741 ggaggagctg ccgggcaatc gtacgcggtt gatcgtcagc gggtactggg cgttccagcc
     4801 gcgctggctg cagccgctca tgaccgtgct ctttctcgat ctccagcact ggatcatgca
     4861 gatgcgccag ttcgcgaacc tgaagcaccg cgtggaacag gcgaagccag cgacccggta
     4921 tccgagcccg atggttgatc tggcggccgc ccgcattcgc tgattagcgc ccgggagatt
     4981 cgtaaatgat caacaagatc ctgctcggct gcggcgccgt ctcgtccgcg ctctatatca
     5041 tctccgacgt gatcgcggcc cggcgatacg acggatacag ctactccgat caatggttca
     5101 gcgaattgac ggccgaaggg gcgccgacca ggccgttgat gacggtgacc aacgagattc
     5161 cctacaccgc tctcatgacc gccttcgcga tgggcatctg gaagacggcc ggtcagaatc
     5221 gcgctcaacg gctcacggcc gcgctgctcg gcggatatgc ggccgccgga atcgcgggtg
     5281 gtgttggctt cccgatggcc acgcgggatg cggaggcttc gctgcgcaat atcatgcata
     5341 tcccgtcgac catggtgatg tccctctgta tcctggcggc gatgggaacc gcttcgaggc
     5401 tgttcgggaa tcgattccgg tactacagct acagcacaat cgcggcggca atcacgttcg
     5461 gtgtgttgac gagctcgcag gccggcaaaa tctccaagaa tcagccgacc ccatgggcag
     5521 gcatcgagga gcgcgtcaac atttacgcaa cgatgctctg gctggcgatg ctttccattg
     5581 gcttgctgcg cgcgctgcgc ccgaccgact cgcgaacggc ggagaagccg gcagttaccg
     5641 cgctgccgat gcacggcctg ccccagtaag ccggaatggc aagcaattgg cccgggatcg
     5701 cgtcaggatt cgacagcgcg tcgtatcatc ttgtgaccgc cgagccaacg caaatcgagg
     5761 gctcgcgcta tgcccatcgt ctagcttctg cccggtccgc acgcatcgtg agcgtgcgca
     5821 gcgggagata cggggcaacc tatgcgactc gagctcgtgg accaggaaga caaccagcgc
     5881 acgatcggcg ccatcgtcgt cacccaggac ggcggcgaga tttcgattga tgccggtccg
     5941 gccgatggac tgccactcgt tcgcctgaaa atctcgcgac aggaggctct caaattgagc
     6001 gcgaacctgc gcgcggtcgc aaacgggcgc gatgaagagg tcctgctcga cgatcagtag
     6061 cagacgctac ccgtatcgaa accgaaatca atatctggac gttgtatcag tagattcgcg
     6121 acgacgtcgt cggcaggttt acaaccgaag cgctgatggt ctcgcgcatc cgaccgcgtg
     6181 agttgtgcga ttgcttcccg cgggtcgtac cgatgtttcc actcaggacg ttagcctgtc
     6241 ctgtgacaga cacaccccat tcgaccccct ggaaccgagc cgcacgtgcg gctcgcctca
     6301 gacccgatcc tcacgatacg cagcgagtgc gaccaccgct gcgttgggct tcctcggcag
     6361 tggtcgacaa tcaaggtgaa acgcccggct gattagcagc cgggcgtttt cgctgtgcac
     6421 catgacgggg cgagccgccc cgagctacgg ttgaagaatc gaatcaggca gccgaggact
     6481 tcgactcgcc gaccggctgc ggggtcttgg cctgctcgac ctcggcgtac ggctggggtc
     6541 cgatctcggc ccagcggcgc gtgaccgcat accggagcgc agccacccgg tcatcgacct
     6601 gcaacttgcg cagcacggac gtcatgtgat tcttgaccgt ctgctcggtg atgaagaggg
     6661 cgtcgccgat ctccttgttg gagtggccca tgaccaggca atcgagtact tccagctcgc
     6721 gcggcgagag ggcgacctcg gccgccggca cgccgttgcc atcgctgagc gtccggaact
     6781 caccgagcac ccgccgggcc agctccggat tcgtcaggac ggcctggtgg agcaggttct
     6841 cgcccttgag cacggagtgg accgtctcca ggattcgcgt cggctcggtg tccttggtca
     6901 ggaacgcggc cgcaccgacc cggacggccg agaagaggcg atcgtcgtcg acgtgcatcg
     6961 agagaaaaat caccgaggtg gtcggccgct cccgccggag ggccgaggcc acatgaatcc
     7021 cggtcacgcc gggcagttgg acgtccatga ggacgaccga tggcttgtga atgtcggcct
     7081 gatgcagggc ttgatggccg ttctcggcct ctgccaccac tgccaggcgc ggatcggatt
     7141 cgagcagtcg gcgaagtcca atgcggaaca ttgggtgatc gtcgacgatc atgatccggc
     7201 gcttcagcat ggcggggtct ccctcatgtc gagtacgttt tgactcaccg gcactccatc
     7261 agagtgatcc ggtagctcac ttatcggcta ttgaaggaag gtcggacagt gtacgtttag
     7321 tgacgcaggt cgcggttttg tcctatacga tcggactatt tcgaatagga ccgtcggtat
     7381 actgagcctg cgttgcgcaa aatgttgtag cgcgcgtaca cctgcgttca gttcttgcgc
     7441 agttcggaaa tggaaggaaa tggcttcacc ctcatcatct aacggacgct catcgatgac
     7501 tccacggcga gtcgttgtca ccggggttgg cgcggtgacg tcactcgggc cgacagcgaa
     7561 gcagacgtgg gaaaagctcc tggcctgcga gagcggcctg aagcggatcg accttgaccc
     7621 ggagcagaac ccgtgcctcg tgcgcggtga cgtggacgat tcgacgatca gcaatcgctt
     7681 cctcgatggc aaggctctgc ggaacacctc ccgtttctcg cggatggcgg tcgaggcagc
     7741 cggggagtcg ctgatcgacg ccgggttgat cggggaggat ggaactccgg tcgccgatct
     7801 ctcggatgcc ggcacggtga tcggaacctg cgtcggcggc gcgcacgatg atctgctgcc
     7861 ggcctggacg acctacgaga accgtggacc ggggcgcgtt cctccgcacc tgcacgtgat
     7921 gttcccgctc aacctggcca gctacacgat ccagtggcgc tggggcatgc agggtccgac
     7981 gaacacggtc aacactgcct gcgccaccgg ctcgcaggcg atcggcgagg ccttccacca
     8041 ggtcaagtac ggcaaggcgg acatcatggt cgccggcgcc gtcgaatcgg attcgcatcc
     8101 gatgttcatc gccggtttcg cggcgatgaa ggcgcttgcg accgacagca acgacaaccc
     8161 gccggcggcg agtcggccgt tcgaccagag ccgggccggt ttcgtgctcg gcgagggcgc
     8221 cggtatggtc gtgctcgagg aatacgagca cgcgaaggcg cgcggcgcga agatctacgc
     8281 cgaagtcctc ggctatgcct cgacgaatga cgccttccat ccaatcgcgc cgcgcccgga
     8341 cggcagcgga tcggccaagg cggtccgcaa cgcgctcaag gatgccggcg tcgaaccgga
     8401 cgaggtcggc cacgtgcagg cgcacgccgc ctcgacgccg gtcggcgatg tcgcggaggc
     8461 gaacgcgatc aaggaggtct tcggcgaccg ggcgacctcg atcccggtga catcgatcaa
     8521 gggcgcgatc ggccactgca
//
LOCUS       DATAOZ010000032         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7120242, whole genome shotgun sequence.
ACCESSION   DATAOZ010000032 DATAOZ010000000
VERSION     DATAOZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7120242"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VHR64_00865"
     CDS             complement(<1..899)
                     /locus_tag="VHR64_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301405.1"
                     /translation="MTTTPSRYQRRSLARCLAVLMLLVVALPMPLLKPAPVVHAASTC
                     LGWTSGGTTFTTRRIQFTSDGVLHLVGCNETFTLTDILNADTANTIVGSEPDNFLQLV
                     DSSQKIWMLNIPLSIEEGATLNLIGGSSGDVNWLRLKSGSAGIIWIRTINGTILIQNS
                     RVSSWDPTTGTYDTTTAGAPGTDETGGGGTKPRSFIFARSYLTAGRAWNAPTSCGSGG
                     GGRDYYEARMDVINSQIDHLGYHGSEAYGASWKVATNDTSLVPPNSRELYNRADVFAT
                     IQNSTFTQNYFGAYTFGGYCMNVT"
     gene            complement(943..2268)
                     /locus_tag="VHR64_00870"
     CDS             complement(943..2268)
                     /locus_tag="VHR64_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX3301406.1"
                     /translation="MSVTRRLFLASGLGMLVAPTRHHRAFAQGTPTPTPKRTLFVDQD
                     HGDDGNSGGQSDPFATIARACQVLEPGDRVEIAAGTYEEKIVIGASGTQDAPIVITAA
                     SGADVQVKKGGIRIAADYVSLENLTVENAGSGDDAGIYITRGQNIALRNMTARKNDGG
                     GVKIKPADGPVVDLLISGGTFADNNGAGIVATGEDVLVGLQLENLTLTDNAGDGLQVE
                     RASQVTVSGISTERNGKDDERNGVFLKQVRGAEIGGVFSEDNGHNGIALRGAENILIS
                     RCISRGNSHHGFDSIEHGKKITYANNVSYANGSGDQDKGLYVTATAGITLQNNILFEN
                     AGDQLAFSNEGGDVTDIASDHNLMYRSDGNRLVRWFADYYSDLAAYQTASGQDAASLS
                     ADPHLTDPKGDNYTPMPDSPVIDAGTEVAQVTDGYSGKAPDIGAIEYRA"
     gene            complement(2265..>3420)
                     /locus_tag="VHR64_00875"
     CDS             complement(2265..>3420)
                     /locus_tag="VHR64_00875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3301407.1"
                     /translation="YQEDPVIFRRALESWRINQPDEIITVIDVTDTTCIDIANEYPDV
                     TVIVTAIPGKRPALVDGILAATTDIVVLVDSDTIFEPEVLKRVLRPFADPKIGGVGTR
                     QKVLVRGSIWQRIADIFLDVRYEDEVPAMTRMGQGLSCLSGRTAAYRRSVLLPMLNEF
                     LAETFLGKQCMSGEDKRLTTLVLKAGYNTYYQGDALVWSTFPAEFSEFIKQRVRWTRN
                     SYRSDLRALWEGWVWKRWYLAFLLIDKSIAPYTLLLGLSYFVYSVFQGDWAVAAIIIG
                     WWLFSRTVKLWPHLRRYPQDFVLLPVFIGVTFLMTFVKAYALATVHHHKWLTRQVEVV
                     NDTVVRSGSDAAVRPNRVPFLTRAVGSAIMITILGAVQFAAWAIYRMVSP"
BASE COUNT          597 a         1034 c         1135 g          654 t
ORIGIN      
        1 gtcacattca tgcaatatcc gccgaacgtg taggcgccga agtagttctg ggtgaaggtg
       61 ctgttctgga tcgtcgcgaa gacatcggcc cggttgtaga gctcccgtga gttcggcggg
      121 accagcgacg tgtcgttcgt ggcgactttc catgacgcgc cgtaggcctc ggaaccgtga
      181 tagccgaggt ggtcaatctg cgagttgatg acgtccatcc gcgcttcgta gtaatcgcgg
      241 ccgccgccac ccgatccgca gctcgtcggc gcgttccagg cccgtccggc cgtgaggtac
      301 gaacgggcga agatgaagga gcgcggcttg gtcccgccgc cgccggtctc gtcagtgccc
      361 ggtgcgccgg ccgtcgtcgt gtcgtaggtg ccggtggtcg gatcccacga ggagacgcgc
      421 gagttctgga tcaggatcgt gccgttgatg gtccggatcc agatgatgcc ggccgagccg
      481 cttttgaggc gaagccagtt gacgtcgccg ctgctgccgc cgatcaggtt cagggtcgca
      541 ccctcttcga tgctgagcgg gatgttgagc atccagatct tctggctgga atcgaccagc
      601 tgcaggaagt tgtccggctc gctgcccacg atcgtattgg cggtgtcggc gttgaggatg
      661 tcggtcaggg tgaacgtctc attgcacccg acgaggtgca gcacgccatc cgaggtgaac
      721 tggatgcggc gggtagtaaa ggtcgtcccg ccggatgtcc aacccaggca ggtcgaggct
      781 gcgtggacga ccggcgcggg tttcagcaac ggcatcggca acgcgacgac cagcagcatc
      841 aagacggcca ggcatctcgc cagggatcgt cgttggtagc gggatggagt cgtggtcata
      901 acctcggcct ttggcctggt gcggggacac ctgccgtgat gtttaggctc gatattcgat
      961 cgctccgata tccggggcct tcccggaata gccgtccgtg acctgcgcga cctcggtacc
     1021 ggcgtcgatc acgggcgaat cgggcattgg cgtgtagttg tcgcctttcg gatcggtcag
     1081 gtgcggatcg gctgaaagcg acgccgcgtc ctgaccactg gccgtctggt atgcggccag
     1141 atcgctgtag taatcggcaa accagcggac caggcgattg ccgtcggacc ggtacatgag
     1201 gttgtggtcc gaggcgatgt cggtcacgtc gccgccctcg ttcgagaagg ccagttgatc
     1261 gcccgcgttc tcgaagagga tgttgttctg cagggtgata ccggcggtag ccgttacgta
     1321 cagccccttg tcctgatcgc cgctgccgtt ggcgtaggag acattgttcg cgtaggtgat
     1381 cttcttgccg tgctcgatcg agtcgaagcc gtgatgactg ttgccgcggc tgatgcagcg
     1441 gctgatcagg atgttctcgg cgccccgtag agcgatcccg ttgtgcccgt tgtcctcgga
     1501 gaagacgccg ccgatctcgg ccccgcgcac ctgcttgagg aaaacgccgt ttcgctcgtc
     1561 atccttgccg ttccgctccg tcgagatgcc tgagacagtc acctgtgacg ctcgctcgac
     1621 ctgcagccca tcgccggcat tgtccgtcag ggtgagattc tcgagctgca gcccgaccag
     1681 cacgtcttcg ccggtggcga cgatgcccgc gccattgttg tcggcgaagg tacctccgct
     1741 gatcaggaga tcgacgaccg gtccgtcggc tggcttgatc ttgacgccgc cgccgtcgtt
     1801 cttgcgggcg gtcatattac gcagcgcgat gttctgaccg cgcgtaatgt aaatgccggc
     1861 gtcgtcaccg ctgccggcat tctcgacggt gaggttctcc agcgagacgt aatccgcggc
     1921 gatccggata ccgcccttct tcacctgcac gtcggcaccc gaagcggcgg tgatgacaat
     1981 cggcgcatcc tgcgtgccgg aagcgccgat cacgatcttc tcctcatagg tgccggccgc
     2041 aatctcgacg cgatcgcccg gctcgagcac ctggcaggcc cgggcaatgg tcgcaaacgg
     2101 atcggattgg ccgccggagt tgccgtcgtc accatgatcc tggtcgacga acaaggtgcg
     2161 cttcggggtc ggtgtcggag ttccctgggc gaaggctcga tgatgacggg tgggagcgac
     2221 aagcatgccg agaccggagg ccagaaacag tcgccgggtc acactcatgg cgacaccatc
     2281 cggtagatcg cccatgccgc aaattgaacc gccccgagga tcgtgatcat gatcgccgac
     2341 ccaaccgcgc gggtcaggaa cgggacgcga ttggggcgga ctgccgcgtc ggatccgctg
     2401 cggaccacgg tgtcgttgac cacctcgacc tgccgggtaa gccacttgtg gtggtggacg
     2461 gtggccaggg catatgcctt gacgaacgtc atcaggaacg tgacaccgat gaagacggga
     2521 agcaggacga agtcctgcgg gtagcggcgc aggtgtggcc agagcttgac ggtgcggctg
     2581 aatagccacc agccgataat gatcgccgcc accgcccagt ccccctggaa gaccgagtaa
     2641 acgaagtagc tcagccccag caacagtgtg tagggcgcga tcgacttgtc gatcagcaag
     2701 aaggccaggt accagcgctt ccagacccac ccctcccaga gggcgcgcag gtcggagcga
     2761 taggagttgc gggtccaccg cacgcgctgt ttgatgaact cggagaactc ggccgggaag
     2821 gtcgaccaga ctagtgcgtc gccctggtag taggtgttgt agccggcttt cagcactagc
     2881 gtggtcagcc gcttgtcctc cccgctcatg cattgcttgc cgagaaacgt ctcggcgaga
     2941 aattcattga gcatcggcaa cagcacggag cgccgatagg ctgccgtccg gcccgagagg
     3001 caagagagcc cttgccccat cctggtcatg gccggcacct cgtcctcata gcgcacatcc
     3061 aggaagatgt ccgcgatgcg ctgccagatc gagccgcgca cgagcacctt ctgccgcgtg
     3121 ccgacaccgc cgatcttcgg gtcggcgaag gggcgaagga cccgcttcag gacttccggc
     3181 tcgaagatcg tgtccgagtc gacaagcacg acgatgtcgg tcgtggccgc gagaatcccg
     3241 tcgacaagcg cgggccgctt cccggggatg gccgtgacga tcaccgtgac gtcaggatat
     3301 tcgttcgcga tgtcgatgca cgtggtatcg gtgacgtcga tgacggtgat gatctcgtcc
     3361 ggctggttga tgcgccagga ctcgagtgcg cgccggaaga tgaccggatc ttcttggtag
//
LOCUS       DATAOZ010000033        14256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1334333, whole genome shotgun sequence.
ACCESSION   DATAOZ010000033 DATAOZ010000000
VERSION     DATAOZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14256
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334333"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VHR64_00880"
     CDS             complement(<1..248)
                     /locus_tag="VHR64_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301408.1"
                     /translation="MAMLSMVTFRAKPGRMSDIVNELKELKEIENRVGTNFRGMRLFE
                     KRIGGPNSGQFAIIFEYEDLASWGATLDAELRDKAFQQ"
     gene            complement(388..1245)
                     /locus_tag="VHR64_00885"
     CDS             complement(388..1245)
                     /locus_tag="VHR64_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX3301409.1"
                     /translation="MGVLIVLGIIVLFAIIILLSAIKVVQEYERGVVFRLGRLAGVRG
                     PGLILLIPFIERMVKVDLRTVTMDVPAQEVITRDNVTIRVNAVAYFRVIDPQASVVNV
                     ADFIRATSQIAQTTLRSILGQSALDELLGEREKINQQLQVIIDEQTEPWGVKVSVVEV
                     KDVELPQSMQRAMARQAEAEREKRAKIIHAEGEFQASQQLAEAAEIINQNPVTIQLRY
                     LQTLTEIGAEKNTTVVFPLPIDMVSAFIAKTTGSSEPAPSEPSLRVMPPAPSVETTSI
                     APPADEDEL"
     gene            complement(1276..2664)
                     /locus_tag="VHR64_00890"
     CDS             complement(1276..2664)
                     /locus_tag="VHR64_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX3301410.1"
                     /translation="MGAIPRLIATLVCTVAVGLALSLIVPATHAQSDQPIVVVAKMDG
                     PITPVMARYINRAISRAEDRSASALVIEMNTPGGLSSAMDDIIDDILQSKVPVIVYVS
                     PANARAASAGVYITYASHVAVMAPSTNIGSASPVMTGSTGNAESDATMKKKVTNDAVA
                     RITNLAQLRGRNVEWAVSAVEDAANITSQQALDMHVIDMIEPNLDQLLADVNGRTVNL
                     ASGPVTLNTANAQIQTVDMGWLEQFLQMLADSTLAYLLVSFGMLGIFIELSHPGISFP
                     GIFGGIALLLGLLGLGSLPVAWGGLAFMALGFVLFVIDIFVASAGLLTIGGLISFLIG
                     SNILISNGAPSDQQIPNYVTWTMTACLAGAALLLGMVVAKSQLRRPKTGKPGMIGQIG
                     VTRTALDPSGMVLVFGELWAATAEQPPIAPGQNVSVTAVNGMSLLVRRATAEEVIAFS
                     DVADRRAVVPVQ"
     gene            3045..4313
                     /locus_tag="VHR64_00895"
     CDS             3045..4313
                     /locus_tag="VHR64_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX3301411.1"
                     /translation="MAKQQSTTTTDVLSPLESLLPDLESFYKDIHSHPELSMQETKTA
                     AKAAERLQSAGFDVTTGVGKTGVVGLLRNGDGPTVMLRADMDALPVKEATGLPYASTV
                     TVTDREGKTVPVMHACGHDMHVTWLTGAATLLAQARDAWHGTLMPVFQPAEETAEGAQ
                     AMIVDGLFTRFPKPDVILGQHVMPSPAGLVGSRTGVITSAADSLEIRFFGRGAHGSMP
                     QASIDPVIMAAATTMRLQTIVSREIAPNEAAVVTIGSLQAGTKENVIPDEAVLKLNVR
                     TYDDGVRERVLSAIKRIAEAEAQASGAPKPPTITPLDNYALVRNDPDATGRVRDAFRR
                     HFAADQVTESGPASASEDFGSFGTEWHVPSVFWFVGGTDPDLYAKAKAENRLGDIPTN
                     HNPHFAPVIHPTLETGVETLVVAAQAWLSA"
     gene            4345..5004
                     /locus_tag="VHR64_00900"
     CDS             4345..5004
                     /locus_tag="VHR64_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX3301412.1"
                     /translation="MQTLVLVLDLVGTFVFALSGAMAAMTNRLDLFGVLSLSFAAGNA
                     GGITRDLIIGATPPAAIDDWRYLGVSLVAGMFTFWCAPLISRLSSPVQILDAGGLGLF
                     AVSGATKALSFHLDPIPAMLLGMLTGIGGGMLRDTLITEIPSVLRENLYAVAALAGAA
                     VVVAGHALGLPATPMALLGAALCIGLRLISIQRGWQLPRASLPAQSPTDARPDDRSKT
                     P"
     gene            complement(5020..5907)
                     /locus_tag="VHR64_00905"
     CDS             complement(5020..5907)
                     /locus_tag="VHR64_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301413.1"
                     /translation="MALVAERVRESFVPVAKGSDQLVWYFFRVTGVLLVGLVFSHLFI
                     THYLHAPSETTFDFVAKRYANPLWRTADWALLLTALWHGLLGARISVNDYIKPHGWRT
                     LVNAVLWVIGFFFTIIGTVTILTFDEAAARNNTGPLAGSFWIGDILGYSLYVLAAITY
                     VAIVLLVIYVARQLSQGNLLFYRGDPGQYAFILHRLTGLGIIGFLLVHILDILLIGLG
                     RDVYDHTVEFYGRLFIIPMEIALVGAVLYHGLNGLRVTILNFWDIGVEKERTLFYAAI
                     IGAALLTIPSLIVILQHEL"
     gene            6148..8055
                     /locus_tag="VHR64_00910"
     CDS             6148..8055
                     /locus_tag="VHR64_00910"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX3301414.1"
                     /translation="MDVINPVVRRWQQDAAADPDTFWARAAEEVHWFRPWDTTFTWDP
                     PTFRWFEGGLTNLSWNCLDYHVERGWGGHAALTALNERGERQTYTYAQLLAGVKSIAA
                     ALRGLGVGKGDRVGIYMPTTPEAIMAMLACTRIGAIHLVVFAGFGSSALAERLSLAGA
                     RVLLTADVTWRKGREINLWEIVGQALADHESPVEHVVILPRGDTLSPLTPGRDLTWEA
                     FLAGGQGHSSGHEVMEANEPAFVLATSGTTAKPKLVVHTHGGYQVGIHSMGKWCFDLK
                     PTDVWWSTSDIGWIVGHSYMVYAPLLAGCTTLAYEGALDHPEPASFYRIIEEHQVSGL
                     FTAPTAVRMLMACGIEPARAADLDSVERVVCAGEVLNVPAWEWIQKEVFADRVPVIDH
                     MWQTETGGPVFSNPYGISLLPIKPGSAGIPMPGIFAEVRTQEGEPANPGEKGIVVLTR
                     PFPSLTATIWNDPERYARDYWGRVPGAYFTGDAASVDEDGYFWFSGRADEIIKIAGHR
                     IGTIEVETALLRHPAVAEAGVTGRPDEIRGEVISAFVVLRAGNEPTPELKSDLLQTVR
                     QELGPVAVIGEINFVKMLPKTRSGKIMRRVFKAVVLDRDPGDISTIEDAGSIDEARAA
                     WQELRAQVHAS"
     gene            complement(8111..8461)
                     /locus_tag="VHR64_00915"
     CDS             complement(8111..8461)
                     /locus_tag="VHR64_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3301415.1"
                     /translation="MTDLLNVTPVQIVNRHVEAMARRDIEGFMADCSGTIRLLSSDGA
                     TQLDGKWAVRETYSGVFDSNPELKVELIDRISVGDWVFDEHLTTGFADGRQVHAVRVY
                     RIEAGRIESIQVFT"
     gene            complement(8488..9369)
                     /locus_tag="VHR64_00920"
     CDS             complement(8488..9369)
                     /locus_tag="VHR64_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301416.1"
                     /translation="MSDFFDIFLLKTVLRMAAPLVFAAMGGLLSERSGVMNIALEGMM
                     LIGAFFGVVGTYYLHSVWLGVLVGMAGAAVAGLIHAFWCITLRADQIVAGTAINLLGS
                     GATVFLLSRIWNQSGQSPKVDRLPTFWGTVSILTPVAFAVVPIVWWFLNRTKEGLHVQ
                     AAGEHPQAAESVGINVGLYRYACVVMSGVLAGLGGIYLSLGELSGFSREMTQGKGFIA
                     LAAVIFGNWNPVGALGACLLFAAAQALPIQAQAKQVDINTDLLLALPYLLTLVAIAGF
                     VRKSRPPEGLGKHATNR"
     gene            complement(9366..10481)
                     /locus_tag="VHR64_00925"
     CDS             complement(9366..10481)
                     /locus_tag="VHR64_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301417.1"
                     /translation="MQLPKQFAGLGKLASTVITSILAVVIALAVGAIVIAISNQNPVD
                     AYRALIDGAFGGRRAIAETLVATTPMILGGLAFAVAARASMFNIGIEGQMVIGSLCGA
                     LVGAADLGLNKWLYVPLAMLVGGVAGGIWGAIPGVMKAKSGASEVISTIMLNYLAFRV
                     SIYMVTSAGSWLSWVDPQLQATKKIAPNARLPIILDRTRLHAGFLVAIAAALILWYVL
                     FRTTFGFRIRTVGLSRGAAEYAGIAWGSTITKAMFLSGLLAGLMGASESLGLLGRHYD
                     NRSGYGFTAIAVGLVGRNHPIGVIFAGLLFGVLRSGSNAMQAQAGTSKELVLILQALV
                     ILSVSALASLEYLRERRSRTAAATPTAAAQPAPGVTA"
     gene            complement(10468..12018)
                     /locus_tag="VHR64_00930"
     CDS             complement(10468..12018)
                     /locus_tag="VHR64_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3301418.1"
                     /translation="MTDPAQPNAVEMRGIVKRFPGVVANDGISFAVRQGEIHALLGEN
                     GAGKSTLMNVLFGIHHPDEGEILVNGQPANFNGPRDAVNAGLAMVHQHFMLIHRFTVA
                     ENVILGSEGSGLLLDRESAERRVSDLANEYGLRLDPKEVVENLPVGMQQRVEIVRALY
                     QGGRTLILDEPTALLTPQEVEELYVILERLRAGGSTIIFITHKLREVAAISDRVTVIR
                     RGKTVDTLKTADTTASQLAELMVGRKVSLQVDRPVGSPEGVLLEAKHLRMRGRGALDA
                     LDDLSLTVRGGEIVGICGVEGNGQTELVESIAGLRKPDEGTIEIAGQDVTGKSPVELR
                     KAGLSYIPEDRHNRGLVLDFNLTENVMLGNIDQPPFSRNGLVDYGEGEAVTEQCMKQY
                     DVRAPGADTLARNLSGGNQQKLIIAREMYRDPSVVLAVQPTRGLDVGAIEFVHKQLVA
                     QRDAGKAILVVSFELDEIIDLSDRILVLYQGRIVGEFASGTVDRATLGLLMGGRSLDE
                     QEPINAVA"
     gene            complement(12097..13188)
                     /locus_tag="VHR64_00935"
     CDS             complement(12097..13188)
                     /locus_tag="VHR64_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3301419.1"
                     /translation="MSDLFDAVLSRRSLVKVSVAAGALASMPFSLTSVGAQDKVVATM
                     VTDTAGIGDQNFNDLAIKGGNEAAAEYGIEWKVIESVDQASYEPNLTQAAEQSQLTVA
                     VGFKLTDALTTVAALYPDKFFLIVDSVVDAGNVRSVLFKEDQIGYLCGVVSGLSTKTN
                     KLGIVGGERIPPVIRYEVGFVAGVKSVNETAEVIINYAGTFNDQAKGKDIAAALYNDG
                     CDIVFPIAGLTGIGAYAAVSELNKPGELWVVGVDTAQDHLAPGYELCVGRKGVDFAVK
                     TACADVANGKFTPGLTTYDLKSGGIGLSLYEDRTAASTNDLARGYEKAIIDGTIVPPT
                     TDDELKAFVVPPQPEPIAATPEASPSPSA"
     gene            complement(13301..14224)
                     /locus_tag="VHR64_00940"
     CDS             complement(13301..14224)
                     /locus_tag="VHR64_00940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3301420.1"
                     /translation="MARPLKVGVQLPEVEYEYTWPQLAEMAKVAEDVGLDSIWLGDHL
                     MYRYQRKEREPRGPFEAWTTLGALAAVTSRVELGPLVASLGFHSPAMIAKKAATIDQI
                     SNGRFILGIGSGWHEPEYKGFGFPFDHRVSRFGEAFAILRELLETGESDFAGGYYTIE
                     GGLLFPKPVREGGIPIMLGSRGPRMLEIALPHIEMWNAWYEDWDNNRTGLARLMAEID
                     AACEKVGRDPKTLIRTICPLVRMKGGRGRISDFPGQAGDNPINGADAAVLAEELNAFA
                     ELGVGHVQLVIDPVNASSIADLRGVLDILDA"
BASE COUNT         2753 a         4531 c         4380 g         2592 t
ORIGIN      
        1 atttgctgga aggctttgtc ccggagttcc gcgtcgagcg tcgcgcccca tgacgccagg
       61 tcctcgtact cgaagatgat cgcgaactgc cccgagttcg gcccgccgat ccgcttctcg
      121 aacaagcgca tgccgcggaa gttggtgccg acccgattct cgatctcctt cagctccttg
      181 agttcgttga cgatatccga catcctcccg ggcttcgccc gaaacgtcac catcgaaagc
      241 atggccatcg cgacctcctc cggggctccg cgggcggcat cggacgaggg cgcactccct
      301 cgtctcgtaa tcccgcattg tttccccgaa tgggtcgatc ttatcaccga cctgtcattt
      361 ggccgctcgg accactgacc actatcgtca cagctcatcc tcatcggcgg gtggcgcgat
      421 cgaggtcgtt tcgacgctcg gcgccggagg catgacccgc agcgaaggct cagacggagc
      481 gggttcagac gagccggtgg tcttggcaat aaacgcgctg accatgtcga tcgggagcgg
      541 gaagacgaca gtggtgttct tctcggcgcc gatctcggtc aacgtctgca ggtagcgaag
      601 ctggatcgtg accgggttct ggttgatgat ctcggccgcc tcggcaagct gctggctggc
      661 ctgaaactcg ccttccgcgt ggatgatctt cgcccgcttc tcgcgctcgg cttcggcctg
      721 ccgggccatc gcccgctgca ttgactgcgg cagctcgaca tccttgacct cgaccacact
      781 gaccttgacg ccccatggct ccgtctgctc gtcgatgatt acctgcagct gctggttgat
      841 cttctcgcgc tcgcccagca actcatccag ggccgattga ccgaggatcg accgcagcgt
      901 ggtctgcgcg atctgcgacg tcgcgcggat gaagtccgcc acgttcacga ccgatgcctg
      961 cggatcgatg acacggaaat aggcgaccgc attgacgcga atcgtgacgt tgtcccgcgt
     1021 gatcacttcc tgcgccggca cgtccatcgt gacggtccgc aaatcgacct tgaccatccg
     1081 ctcgatgaac ggaatcagca ggatcaggcc cggaccccgc acgccggcca ggcgaccgag
     1141 ccggaagacg acgccccgct cgtactcctg cacgaccttg atcgcgctca agagaatgat
     1201 gatggcgaag aggacaatga tgcccagaac gatcaatacg cccatcgcga tgccttcctc
     1261 atgtactcac ccgcgctatt gcacaggaac gactgctctt cgatctgcga catcgctgaa
     1321 cgcgatcact tcctccgctg tcgcccgccg gaccagcagc gacatcccgt ttacggcggt
     1381 cacagacaca ttctgaccgg gcgcgatcgg cggttgttcc gcggtcgccg cccagagctc
     1441 gccgaatacg agaaccatac cgctcgggtc caatgcggtc cgcgtcacgc cgatctgccc
     1501 gatcatgccg ggcttgccgg tcttcggccg gcgcagctgg ctctttgcga cgaccatgcc
     1561 gagcaggagt gccgcgccgg ccaggcaggc ggtcatcgtc cacgtcacgt aattcgggat
     1621 ctgctgatcg ctcggcgcgc cattcgagat caggatgttg gatccgatca ggaagctgat
     1681 caggccgccg atcgtcagta accccgccga cgcgacgaag atgtcgatca caaagagaac
     1741 aaagccgagc gccatgaagg ccagtccgcc ccaggctacc ggcaagctgc ccaaaccgag
     1801 cagtccgagc agaagcgcaa tcccgccgaa aatgcccggg aaactgatgc cgggatgcga
     1861 gagctcgatg aagatcccga gcatcccgaa gctaaccagc agataagcga gggtcgagtc
     1921 ggccagcatc tggaggaact gctcgagcca gcccatatcg accgtctgga tctgcgcgtt
     1981 ggcggtattg agcgtgactg ggccggatgc gaggttgacc gtgcgaccgt tgacatctgc
     2041 caggagctga tcgaggttcg gctcgatcat atcgatcacg tgcatgtcga gcgcctgctg
     2101 ggacgtgatg ttggcggcgt cctcgaccgc gctgaccgcc cactcgacat tgcggccgcg
     2161 cagctgggcg aggttggtga tccgggcgac ggcgtcgttc gtcaccttct tcttcatcgt
     2221 cgcatcgctc tcggcgttgc cggtgctgcc ggtcatgacg ggcgaggcgg agccgatgtt
     2281 ggtcgagggc gccatgaccg cgacgtgcga ggcgtaggtg atgtagaccc cggccgaggc
     2341 cgcgcgggca ttcgccggcg agacgtagac gatgaccggg accttgctct ggaggatgtc
     2401 gtcgatgatg tcatccatcg ccgacgagag accgccgggc gtgttcatct cgatcacgag
     2461 cgcgctggcc gagcggtcct ccgccctcga gatggcgcgg ttgatgtacc gggccatgac
     2521 cggcgtgatc ggtccgtcca tcttggcgac gacgacgatg ggctggtcgg attgcgcatg
     2581 ggtcgcgggg acgatcaggc tcagggcgag cccgactgcg acggtgcaga cgagagtagc
     2641 gatgaggcgt gggatggcgc ccatggcgtc acctccttca cctctaacta tacagaagat
     2701 ggaacagcgg ttctaaaggc catacccccc tctctatctc acgatgtgca gtggttccag
     2761 gatctgcaac agcctggtcg ccgggccgac gtcaagtgct cctgggtcgc ccggatgtag
     2821 cgcttcctga tcggtcgggt gttcggcgga gtaccgtttc tgcaatcgga gtagcaggac
     2881 gggcgcatgc ccgccgcgta ctcacaaatc agtgcgcccg ggtccgcctc gcacggtgca
     2941 gcttgccaac cgacagtcgc ttatgacttg actttgcgga ccggtacccg acctgccaga
     3001 acggcaaggc agaccgtaca tccccgcaag aaagggcctc tacgatggcc aagcaacagt
     3061 cgactacgac caccgacgtg ctgtcccccc tcgaatcctt gcttccggat cttgagagct
     3121 tctacaaaga cattcattcc catcctgagc tttcgatgca ggagaccaag acagcggcga
     3181 aggcggctga gcggctacaa agcgccggct tcgacgtgac caccggagtg gggaagaccg
     3241 gcgtcgtcgg cttgctgcgc aatggcgacg gaccaacggt catgctccgc gccgatatgg
     3301 acgcgctgcc ggtcaaggaa gccaccggac taccgtatgc cagcacggtg accgtcaccg
     3361 accgcgaggg gaaaaccgtg cctgtgatgc atgcctgcgg ccacgacatg cacgtgacgt
     3421 ggctaaccgg cgccgccacc ttgctcgcgc aggcacgcga tgcctggcat gggacgttga
     3481 tgccggtctt ccagccggcg gaggagacgg ccgagggagc ccaggcgatg atcgtcgacg
     3541 gcctcttcac ccgattcccc aaacccgacg tcatcctcgg tcaacatgtc atgccctcac
     3601 cggcgggcct tgtcggttcg cgcacgggcg tcatcacatc cgccgccgac agcctggaga
     3661 ttcgattctt cgggcgtgga gcgcacggat cgatgcccca ggcgagtatc gaccctgtga
     3721 tcatggcagc ggcaacaacg atgcgactgc aaacgatcgt ctcccgggaa atcgcgccga
     3781 acgaggccgc cgtcgtgacc attggctcgc tccaggccgg cacaaaggag aatgtcatcc
     3841 ccgacgaggc cgtgctcaaa ttgaacgtcc ggacctacga cgatggcgtg cgtgagcggg
     3901 tgctgagtgc gatcaagcga atcgccgagg ccgaagccca ggcatccgga gcacccaagc
     3961 caccgacgat cacgccgctc gacaactatg cgctggtgcg gaacgatccg gatgcgaccg
     4021 ggcgcgtcag ggacgcgttt cgccggcact tcgccgcaga tcaggtgacg gaatcgggac
     4081 ccgcgtcggc gagtgaagac ttcgggtcgt tcggcaccga gtggcacgtc ccgtcggtgt
     4141 tctggttcgt gggcggcacc gatcccgatt tgtacgccaa agcaaaggcg gaaaaccgcc
     4201 tcggggatat tccaaccaac cataaccccc actttgcccc agtgattcat cctacactcg
     4261 agacaggcgt ggagaccctg gtggtggctg cccaggcatg gctctccgcc tgaggcatcg
     4321 tatccacaaa ggatctggcg acccgtgcaa acgctggtgc tcgtgctcga cttagtcgga
     4381 acattcgttt ttgcgctcag cggcgccatg gcggctatga cgaatcgcct ggacctgttc
     4441 ggtgtgctga gtttgtcttt cgcggcggga aacgccggcg gcatcactcg ggatctgatc
     4501 atcggggcga cgcctccggc agcaatcgac gactggcggt atctcggagt gtcactcgtc
     4561 gccggcatgt tcactttctg gtgtgccccg cttatcagcc ggctgagcag cccggtgcag
     4621 atccttgatg ctggcgggtt gggactcttc gcagtctcag gcgcgacgaa ggcgctcagc
     4681 tttcacctcg acccgattcc ggcgatgctg cttggcatgc tgacgggcat tggtggtggg
     4741 atgctgcgcg acacgctcat tacggagatc ccctccgtcc tgcgcgagaa cctctatgcc
     4801 gtcgcagccc tggcgggagc cgccgttgtc gtcgctggtc acgccctcgg gcttccggca
     4861 actccgatgg cacttctcgg cgcggcactc tgcatcggac tccgcctcat ctcaatccag
     4921 cggggctggc agctcccccg ggcctcccta ccggcgcaat ctccgacgga cgcccgaccg
     4981 gatgatcgtt cgaaaacgcc ctgatttggc gggaactgcc tagagctcat gttggagaat
     5041 cacgatcagg ctggggatgg ttaggagggc ggcgccgatg atggcggcgt agaagagggt
     5101 acgctccttt tcgacgccga tgtcccagaa attcaggatc gtcacccgca gtccgttcag
     5161 cccgtggtag agcaccgcgc cgaccagcgc gatctccatc ggaatgatga agagtcgccc
     5221 gtagaactcg acggtgtggt cgtaaacgtc ccggccaaga ccgatcagca ggatgtcgag
     5281 gatgtggacc agcaggaagc cgatgatgcc gagtccggtc agccggtgca ggataaaggc
     5341 gtattggccg ggatcgccgc ggtagaaaag caagttgccc tggctgagtt gccgggcgac
     5401 gtagatcacg agcaggacga tcgcgacgta ggtgatcgcg gccagcacgt agagcgagta
     5461 gccgaggatg tcaccgatcc agaacgagcc ggccagcggg cccgtgttgt tgcgcgccgc
     5521 cgcctcgtcg aaggtcagga tggtgaccgt accgatgatc gtgaagaaga agccgatcac
     5581 ccagagcacg gcgttgacga gggtccgcca gccgtgcggc ttgatgtagt cgttgaccga
     5641 gatccgggcg ccgaggagac cgtgccagag cgcggtcagg agcagggccc agtcggcagt
     5701 gcgccagagc ggattggcgt agcgcttggc gacgaagtcg aaggtggttt cggacggcgc
     5761 gtgcaggtag tgggtaatga agaggtggct gaagaccagt ccgacgagca aaacgccagt
     5821 aacccggaag aagtaccaga cgagctgatc gctccccttg gccacgggta cgaacgactc
     5881 gcgcacccgc tctgctacca gcgccatgct gattccaccc tcccctgacg tcaagtccga
     5941 ctcgcgcggc aatcgcgcac tgaacggcat tgtacgacct gaatcgctcc cgtctgagcg
     6001 gctgatccgc aatccgcgac gggccccctc gtccgcttga catagccggg cgtgagccta
     6061 tagtgcccag atagccattc ctccaaacct ctgcgggacc gaccggtccc tcgtcctccc
     6121 cgctcgtgac acaggaggcc gcacgcaatg gacgtcatca accccgtcgt caggcgctgg
     6181 cagcaggatg cagccgccga tccagacacc ttctgggcgc gggctgccga ggaggttcac
     6241 tggttccggc cgtgggacac cacgttcacc tgggatccgc ccacgtttcg ctggttcgaa
     6301 ggcgggctga ccaacctctc ctggaactgt ctcgactatc acgtcgagcg tgggtggggc
     6361 ggacacgccg cgctgactgc cctcaatgag cgcggcgagc ggcaaaccta cacgtacgcg
     6421 cagctcctgg ccggggtcaa atcgatcgcg gccgctctgc gcggtctcgg cgtcggcaag
     6481 ggtgatcggg tcggcatcta catgccgacg acgcctgaag cgatcatggc catgcttgcc
     6541 tgcacgcgga tcggcgcgat ccacctcgtc gtcttcgcgg gcttcggcag cagcgcgctg
     6601 gcggagcggc tgagcctcgc gggagccagg gtgctgctca ccgcggatgt gacctggcgc
     6661 aaggggcgcg agatcaacct ctgggagatc gtcggccagg cgctcgccga tcacgagtcg
     6721 ccggtcgagc acgtcgtgat tctcccgcgc ggcgacactc tctctcccct gacgccgggt
     6781 cgcgatctca cgtgggaggc attcctcgcc gggggacagg ggcattcatc cgggcatgag
     6841 gtgatggagg ccaatgagcc ggcattcgtg ctggcaacat ccggcacaac ggccaaaccc
     6901 aagcttgtcg tgcatactca cggcggctac caggtcggca ttcacagcat gggcaaatgg
     6961 tgcttcgacc tgaagccgac cgacgtctgg tggtcgacat cggatatcgg ctggatcgtt
     7021 ggtcacagct acatggtcta tgccccgctc ctcgccggct gcaccacgct ggcttacgag
     7081 ggagcgctcg accatccgga accggcatcg ttctaccgga tcatcgaaga gcaccaggtg
     7141 agcggactgt ttacggctcc caccgcggtc cggatgctga tggcctgcgg catcgaaccg
     7201 gcccgagcgg cagacctgga ctcagtcgag cgcgtcgtct gcgccggcga ggttctcaac
     7261 gtaccggcct gggaatggat ccagaaggag gtcttcgccg accgcgtccc ggtgatcgac
     7321 cacatgtggc agacggagac gggcggtcct gtcttcagca acccatacgg catcagcttg
     7381 ctgccgatca aacccggctc ggccggaatt ccgatgccgg gcatcttcgc ggaggttcgg
     7441 acgcaggaag gcgaaccggc caacccgggc gaaaagggca tcgtcgtcct gacccggccg
     7501 ttcccgagtt tgacggcgac gatctggaac gaccctgagc gctatgcccg cgactactgg
     7561 gggcgcgtgc ccggcgctta cttcacgggc gacgcggcat ccgtcgatga agacgggtat
     7621 ttctggttca gcggccgcgc cgacgagatc atcaagatcg cgggccaccg catcggcacg
     7681 atcgaggtcg agaccgcgct cctgcggcat cctgcggtcg ccgaagccgg tgtcaccgga
     7741 cgaccggacg agattcgggg cgaggtcatc tccgccttcg tcgtcctccg cgccgggaac
     7801 gaaccgacac cggagctaaa gagcgacttg ctccagacgg ttcgccagga gctgggtccg
     7861 gtggcagtca ttggcgagat caacttcgtg aagatgctgc ccaagacgcg cagcggcaag
     7921 atcatgcgac gggtcttcaa ggcggtcgtg ctcgaccgcg atccgggcga catctcgaca
     7981 atcgaagacg ccggatcgat cgacgaggcg cgggcggcat ggcaggagct ccgcgcgcag
     8041 gtgcacgcca gttaagcgtg gtcgggagcc cgattgatga cggagggtgc caattccctc
     8101 agtttgtctg ttacgtaaag acctggatgg attcgatgcg gccggcttcg atgcggtaga
     8161 cgcggacggc gtggacctgg cggccgtcgg cgaaaccggt cgtgaggtgc tcgtcgaaga
     8221 cccaatcgcc aacgctgatc cggtcgatca gctccacctt gagctcggga ttcgagtcga
     8281 agacgccaga ataggtctcg cggacggccc atttgccatc gagctgggtc gcgccgtcgg
     8341 agctgaggag ccggatcgtc cccgagcaat cggccatgaa gccttcgatg tcgcgccgtg
     8401 ccatcgcctc gacatggcga ttgacgatct ggaccggcgt gacattcaac aagtcagtca
     8461 atgtgtgtgc tccctaccct taaccagcta tcgattcgtc gcgtgtttgc cgaggccttc
     8521 cggcgggcga gacttccgaa cgaatccagc gatggcaacc agtgtcagca ggtacggcag
     8581 tgcaagcaac agatcagtat tgatatcgac ctgttttgcc tgcgcctgaa tcgggagggc
     8641 ttgggccgcc gcaaacaaga ggcatgcgcc aagcgcgcca acgggattcc agttaccgaa
     8701 gatcaccgcc gcgagcgcga tgaagccctt gccttgcgtc atctcacgac tgaatccgga
     8761 cagctcacca agcgacaggt aaatgccgcc caagccggcc agtaccccgc tcataaccac
     8821 acaggcgtag cgatagagac caacgttgat gcccacgctt tctgccgcct gcggatgctc
     8881 gcccgccgcc tgaacgtgca ggccctcttt tgtccgattc aagaaccacc agacaatcgg
     8941 cacgaccgca aatgccacgg gagtcaggat gctgactgtt ccccaaaatg tcggcagtcg
     9001 atccaccttt ggcgattggc cggactggtt ccagatcctc gagagcagga acaccgttgc
     9061 accggatcca agcaagttga ttgcggttcc ggctactatc tggtcggctc gcaaggtgat
     9121 gcaccagaag gcgtgaatca ggccggcgac tgccgcacca gccatgccga ccaatacgcc
     9181 gagccagacg ctatgcaggt agtaggtgcc aaccacgccg aagaacgcgc caatcagcat
     9241 catgccttcg agcgcgatgt tcattacgcc ggaccgctcg gaaagcaatc cgcccatcgc
     9301 cgcgaatacc agcggcgcgg ccatcctgag aaccgtcttt aacagaaata tgtcaaagaa
     9361 gtcgctcacg cggttacccc cggggccggc tgagcggcag cagtcggagt cgccgcggcc
     9421 gtgcgtgacc ggcgctcccg cagatactca agcgacgcca atgccgagac agagaggatc
     9481 acaagcgcct gcagaatcaa taccaattct ttggatgtgc cggcctgggc ttgcatcgca
     9541 tttgaacccg atcggagcac gccgaacagc aacccggcga agataacgcc aataggatgg
     9601 ttcctgccta ctagtccaac ggcaattgcg gtaaatccgt agcctgaacg gttgtcgtag
     9661 tgacggccga gcaagccgag cgattccgac gcgcccatca atccggccag cagcccgctc
     9721 aggaacatcg ccttggtgat cgtcgagccc caggcgattc cagcatattc ggctgcgccg
     9781 cgactgaggc caacggttcg aatccggaat ccgaacgtag tccgaaacag cacgtaccaa
     9841 aggatcaggg ccgctgcgat cgccacaagg aacccggcgt gcagtcgggt tcggtccagg
     9901 atgatcggca gacgcgcgtt cggagcaatc ttcttggtcg cctgcaattg aggatcaacc
     9961 caggacagcc aggaaccggc cgaagtgacc atgtagatgc tgacccggaa cgccagatag
    10021 ttgagcatga tcgtcgaaat gacttcgctc gcacctgatt tggctttcat gacaccgggg
    10081 atggcgcccc agatgccacc ggcgactccc ccaaccagca tcgccagcgg tacgtagagc
    10141 cacttgttca atccgagatc tgccgcgccg accaacgcgc cgcaaagact gccgatcacc
    10201 atttgacctt cgatgccgat attgaacatc gaggctcggg cagcgaccgc aaaggcgagc
    10261 ccgcccagga tcatcggcgt cgtcgcaacc agcgtttcag cgattgctcg ccggccgcca
    10321 aatgcgccat cgatcaatgc ccggtaggcg tcgaccggat tctggttgga aatggcgatc
    10381 acaatcgcac cgaccgccag cgcaattacg acagccaaaa tggaggtaat gaccgtagat
    10441 gccaatttgc cgagccccgc aaactgctta ggcaactgca ttgatcggct cctgctcgtc
    10501 caaactgcgt ccacccatca gcagacccag cgtcgctctg tcgacagtac cgctggcaaa
    10561 ctcgcctacg atacgtccct ggtacagaac aagaatgcgg tcgctaaggt cgatgatttc
    10621 atcgagctcg aatgagacaa caagaattgc cttgccggca tcgcgctggg ccacaagttg
    10681 cttgtgcacg aactcgatgg cgccgacatc gagtccgcgc gtcggctgca cggcgagcac
    10741 gacgctgggg tcgcgataca tctcgcgagc aatgattagc ttctgctgat tgcccccgga
    10801 cagattgcgt gcaagggtgt cggctccggg agcgcgcacg tcgtattgct tcatgcattg
    10861 ctcggtaacg gcttcgcctt cgccgtaatc gacgagtccg ttcctggaaa agggcggttg
    10921 gtcgatgttg cccagcatca cgttctcggt caggttgaaa tcgagcacga gaccacgatt
    10981 gtggcggtcc tccgggatgt acgacaggcc ggccttacgg agctcaaccg ggctctttcc
    11041 ggtaacgtcc tggcccgcga tctcgatggt gccctcatcg ggcttgcgaa gcccggctat
    11101 cgactcgacc aactcggtct gtccattgcc ctcgactccg caaatgccga cgatctcgcc
    11161 gcctcggaca gtcaagctca gatcatcgag tgcatcgaga gcgccacggc ccctcatccg
    11221 caggtgcttc gcttcgagga gcacaccctc cggactacca acgggtcggt cgacctggag
    11281 gctcaccttg cgtccaacca tcagctcagc cagctgggag gccgtggtgt ccgcggtctt
    11341 gagggtgtcc accgtcttgc cgcgccggat cacggttacg cgatcgctga tggcggcaac
    11401 ttcgcgcagc ttgtgcgtga tgaagatgat tgtgcttccg ccggcccgga gacgctcgag
    11461 gatgacgtag agctcttcga cctcctgcgg cgtaagcagt gcagtcggtt catcgaggat
    11521 cagcgtccga ccaccctggt agagcgctcg cacaatctcg actcgttgct gcattccgac
    11581 cggcagattc tcgacgacct cttttggatc aagtctgagt ccgtattcgt tcgccaagtc
    11641 gctgacccgc cgctcagcag attcccgatc cagtaacaat ccggaaccct ctgagccaag
    11701 aatcacattc tctgcgaccg tgaaccggtg gatcagcatg aaatgctggt ggaccattgc
    11761 caggccagca ttgacggcat cacgcgggcc attgaagttc gccggctgac cgttcacgag
    11821 gatctcgccc tcatcgggat gatgaatccc gaagagaaca ttcatgagcg tggacttgcc
    11881 cgcgccgttc tcgccgagca gggcgtggat ttccccttgg cgcactgcga aggaaatgcc
    11941 gtcgttggca accacacccg gaaatcgctt tacgattccg cgcatctcca cggcattggg
    12001 ttgagccggg tcggtcatcg agcgagcctc gtgtcatacg gaaacggacg gctacagtgt
    12061 acctgtagcc gtccgttaaa cattgtgctg ctgaacttat gccgacgggg agggacttgc
    12121 ctccggagtg gcggcaattg gctcgggctg cggtggcacg acgaacgcct tgagctcgtc
    12181 atcggtcgtc ggcggcacga tcgtgccgtc gatgattgcc ttctcatagc cgcgggccag
    12241 atcgtttgtc gacgcagcgg tgcgatcctc gtagagcgat agtccgatac cgccggactt
    12301 gagatcgtac gtggtcagcc ccggcgtgaa cttgccattc gcgacatcgg cacacgccgt
    12361 cttcaccgca aagtcgacgc ccttccggcc gacacagagc tcgtatccgg gggcgaggtg
    12421 atcctgcgcg gtgtcgacgc caacgaccca aagctctcct ggcttgttga gctcggacac
    12481 tgcggcataa gcaccaatgc cggtcagtcc ggcgatcggg aaaacgatat cgcagccgtc
    12541 gttgtaaagc gctgccgcga tgtccttgcc cttcgcctga tcgttgaagg tgccggcgta
    12601 gttgatgatc acctcggcag tctcgttgac cgacttgacg cctgccacga agccgacctc
    12661 atagcggatg accgggggaa tccgttctcc tccgacgatg ccgagcttgt tggtcttggt
    12721 cgacaggccc gaaacaacac cgcagaggta accgatctga tcctccttga agagcaccga
    12781 tcgcacgtta ccagcatcca ccaccgagtc gacgatgagg aagaacttgt ccgggtagag
    12841 tgcggcaacc gtggtcaacg catctgtgag cttaaagcca acagcgacgg tcaactggct
    12901 ctgctcagct gcctgggtca ggttcggctc atacgacgcc tgatcgactg actcgatgac
    12961 cttccattca attccatact cggcagcagc ctcgttaccg cccttgatgg caaggtcatt
    13021 gaagttctgg tcgccaatac cggcggtgtc cgtcaccatc gtggcgacga ccttgtcctg
    13081 cgcgccgaca ctggtcaatg aaaatggcat cgacgcaagt gcgcccgctg cgacggatac
    13141 tttgaccagc gatcgacgag acagcaccgc gtcaaacaga tctgacattg agcgtttctc
    13201 ctccaaacgt cgtgccttac ggtatgtccg agacaggact tacgaagcgt acccgaccgg
    13261 caagtacacc taccagatat gccatccggt caatgtgggt ctaggcatcc aggatatcga
    13321 ggacacccct gaggtcggcg atcgagctcg cgttgaccgg atcgatgacc aattggacgt
    13381 gaccgacacc gagttccgcg aaggcattca gctcctcggc caggaccgcg gcatcggcgc
    13441 cgttgatcgg gttgtcgccg gcttgacccg ggaaatcgct gatccggccc cggccgccct
    13501 tcatgcggac gagcgggcag atcgtgcgga tcagggtctt tggatcgcgg ccgaccttct
    13561 cgcaggcggc gtcgatctcg gccatcagcc gggcgaggcc ggtccgattg ttgtcccagt
    13621 cctcatacca cgcattccac atctcgatgt ggggcagcgc gatctcgagc atgcggggtc
    13681 cgcgcgagcc gagcatgatc gggatcccgc cctcacgaac tggcttcggg aagagcagtc
    13741 caccttcgat cgtgtagtac ccgccggcga agtcagactc gccggtctcg agcagctcgc
    13801 gaagaatcgc aaaggcctcg ccgaagcgac tgaccctatg atcgaacggg aaaccgaagc
    13861 ccttgtactc cggctcgtgc cagccggagc cgatcccgag aatgaagcgg ccattgctga
    13921 tctggtcgat cgtggcggcc ttcttggcaa tcatggccgg gctatggaag ccgagactgg
    13981 cgaccagcgg gccgagctca acccggctcg tgaccgctgc caacgcgccg agggtggtcc
    14041 aggcctcgaa ggggccacgc ggctcccgct ccttgcgttg gtagcggtac atcaggtgat
    14101 cgccgagcca gatcgagtcg agcccgacat cctcggccac tttcgccatc tcggccaatt
    14161 gcggccacgt gtactcgtac tcgacttcgg ggagctgcac cccaaccttc aacggccgcg
    14221 ccatggcgtt ccttccttga tagcgactcc gataga
//
LOCUS       DATAOZ010000034         5855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6332741, whole genome shotgun sequence.
ACCESSION   DATAOZ010000034 DATAOZ010000000
VERSION     DATAOZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6332741"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(971..1393)
                     /locus_tag="VHR64_00945"
     CDS             complement(971..1393)
                     /locus_tag="VHR64_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301421.1"
                     /translation="MQDLIHLADLIHVSNYVNGRISQIIGRPANIGALGEHIASRIFE
                     IDLHENAVTKASNGTFRLPPALAGRSGSIKFYSRMGGLFDMAASVDRASHPDYYLVLS
                     GPIPKSATTKGTQAPLVIDRVYLFDADLLLSKLVDRNP"
     gene            1549..2520
                     /locus_tag="VHR64_00950"
     CDS             1549..2520
                     /locus_tag="VHR64_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301422.1"
                     /translation="MPITFGRVVHPPFRDRLIPDSATRAWDDLGQRTPVGVCMHTMVG
                     TLWGTDAYFRSGAPALTDYGIGGATDGPDWDGVILRWNDPCGRRSGWANGGADGLEGD
                     GPLFVRTLGINAINRDLVSIERSDGRDITTPLSTKQFESLCQLTAYWFDQAKVPWDRF
                     PVNPNIGGGIVTHFLHKEFATKGCPWAAVEDRVDEIQNRVRAILKAAQTEDVSPPIVP
                     SPPKPDHAWWPQGYAIDALTERFGGLVRHHPDGRTQRMGFDSRGVISNAWVARGVAEQ
                     MTVRQLPAARHWWNHPTGDGGQLDLITFANRWLLLRPDRNIAWRWAD"
     gene            complement(2573..3472)
                     /locus_tag="VHR64_00955"
     CDS             complement(2573..3472)
                     /locus_tag="VHR64_00955"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3301423.1"
                     /translation="MAEWTWELLAGPETITEGPAWDGEGLFFSSIVANEIRRYDRASG
                     EISTVYHYTNATNGLIFGLDGMLYACEQATGAIVRYNTRGEKTILRAKFNEMRLNSPN
                     DLVLDSQGRIWFTDPRYGDQTGRELTHDSVYRLTPTEDDALPWPIERLTSDTTRPNGL
                     LLAPDERTLFVAQSDYSGDRQLRAYPIHLDGSLESPKVLHDFGDARGIDGMCWDSEGF
                     IVATCGWAQSGPGPRIAIFAQDGTVIEEHRLPDGAPTNCVFGGPNLDELYVTTLEGHL
                     YRVPNTGRTGILAAPGVRPFVGS"
     gene            3606..3866
                     /locus_tag="VHR64_00960"
     CDS             3606..3866
                     /locus_tag="VHR64_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301424.1"
                     /translation="MLDDTVIIALVPGCVEVAKRSGLPARFAPIAAILTALVLVSLSQ
                     VADAPGPITLALIARLLLTGLIDGLAAIGLYRLVPKGAMTAT"
     gene            4005..>5855
                     /locus_tag="VHR64_00965"
     CDS             4005..>5855
                     /locus_tag="VHR64_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX3301425.1"
                     /translation="MARKGKNVRNTSTPVAAVDVGSNSIKMTVARLRRGTLDILAEES
                     ETVRLGAGLDSTRTLAQDRIAAAIETLSRFAGIARGHGATRLLGVATEATRAATNGEV
                     FLNRAREETGWDLRVISGDEEAGLTFRGLAVIGDMTGSVVVADIGGGSTELIAAESSA
                     VAMFRSIPLGSGRLTDRLITHDPPVPAEIEACADAATGQLGAVPFPVSNPDQMILLGG
                     TAEALARLVPDRDHISTTDLETALCRLMEEPAAPLAESTGIAEARARVLPAGVAIMRA
                     MAQMLHPANMRLAASGIRTGLILTALDEASRIPVDPCAAMSGRIASLWEEVAATMPAA
                     IAGEDPEGVHKMRVASRRLRAAMDIATPCFPTKWYRSLHRTARQVTRSAGAVRDREVL
                     LLFLKSERKKKLTPEMRAAIDHWSAELSVERELARVTMLDFLAEFGANDVLAKARRRF
                     LARGAPSGKSCRKCRGKADVMLIDPERSWTDNASRVLEPRIHDLFSFGDAIADPEAIE
                     AHHSARIAGKRLRYTLELFADVFGKPGTHALDDLTDLQDSLGKIHDTDVRIALIADEI
                     KIQAEGHDPDLTIQAGLEQLAERFQRSRHREHATLARMWQRDAANFEQELI"
BASE COUNT         1070 a         1904 c         1841 g         1040 t
ORIGIN      
        1 cggcgacggt cgcgccaacg ccggctgcct tcagtaaggt gcgacggccg atcgactgct
       61 tggtcgtgac ttcagcaacc tggtcggtga cctcggcatc gggacagaaa tcgtcgagct
      121 ggtcgtcctg gttctgacat ccgcatccca tgatgatctc ctttgctcaa gcgacatgct
      181 gggcctgctc ggcgctcccc gacggtgggg agcgataaca tgcgtgcggg gtcagtccgg
      241 accactactg acgctagggc gccggggcaa aggtgggacc gatctcgcat ttggtcaggg
      301 agatcgggga ggattgcgtc cgtccggggc gcggactaca ctggggattc ctccgccgat
      361 cttgttgaga tcgtggggtc attcgaggcg ggaagggcct gatcgttcgc gcggtggggc
      421 ctcactcgcc tcgtttcttt gcgcgaaagc ggtggtcctc gactctgcca ttccattgcg
      481 cggtcccctc ctgacatggc acatgccatc gaggacgatt acgttcagtt gcgctacaag
      541 tgaatgattc cgccgaatgc tggtgagtag atgaaattcg cagcgacacg catcggcgaa
      601 gcaatcgcag gttggacctg attcctgcgg ggctgaaccg gtgccgaacg aggatggcgg
      661 ctactaggtg agcgcatcgt agtactgcaa cagccctaag tcaatcgtcc cgggtcccgg
      721 aatccgaaat tcctatacgt tcacagtacc ggaaaacaat ggcggaaaag accaaacact
      781 tagcgcggcg acatagacga tctgcataca agatatcgcc ctgcgactaa ccgttctcac
      841 tcgggtgccg caaaaagttg cagcgcccac cgctgctcct cgctcacgat cgacgcttga
      901 ttgacttgat ccgggtagat catcgccact tcccacacgt gcttccggac actggttgca
      961 attccggggt ttatgggttt cggtccacaa gcttagaaag caacaggtcg gcgtcgaaaa
     1021 gatagacacg gtcgatcacc agcggtgcct gagtgccttt ggttgtggct gacttcggaa
     1081 tgggtcctga taagaccagg tagtaatcag ggtgcgaggc acggtccacg cttgcagcca
     1141 tgtcaaagag accgcccatt cgactgtaga acttgatact gcccgaccgg ccggccaaag
     1201 ccggtggcag tcgaaaggtg ccgttcgagg ccttcgtcac cgcgttttca tgcagatcga
     1261 tttcgaaaat ccgcgaagcg atgtgctcgc caagtgcgcc gatattcgcc ggtcgaccaa
     1321 tgatctggct tattcgtccg ttgacgtaat tactcacgtg tatcaaatcg gcgaggtgga
     1381 tcaaatcctg catcgcatat ccccctgatc ggcaccaacc gctagcccaa atcctacgcg
     1441 caacccgttg atcagacgca ggcagattgc catctgcccc tacaaccccg cgcctccgcg
     1501 cctcccgact ccgcgcccca actgctcgcc taatcgaaag gagcccccat gcccatcacc
     1561 ttcggacgcg tcgtccatcc accattccgc gaccgcctga tcccggatag cgctactcgc
     1621 gcctgggacg acctcggcca gcgcaccccg gtcggcgtct gtatgcacac gatggtcggc
     1681 acactctggg gcaccgatgc gtacttccgc tccggcgcgc cggccctgac cgactacggc
     1741 attggcggcg cgaccgacgg gccagactgg gatggagtga tcctgcgctg gaacgaccca
     1801 tgcggccgcc gctccggctg ggccaacggc ggcgcggacg ggctcgaggg cgatggtcca
     1861 ctcttcgtcc gcacgctagg catcaacgcg atcaaccgcg acctggtcag catcgagcgc
     1921 tccgatggcc gcgacatcac cacgccgctc tcgacgaagc agttcgagtc gctctgccag
     1981 ctcaccgcgt attggttcga tcaggcaaag gttccgtggg atcggttccc ggtcaacccg
     2041 aacatcggcg gcgggatcgt cacgcacttc ctgcacaagg agttcgcgac caagggctgc
     2101 ccatgggccg cggtcgaaga tcgagtcgac gagatccaga accgggtccg cgccattctc
     2161 aaggccgcgc agaccgaaga cgtcagtccg ccaatcgttc cgtcaccgcc gaaaccggac
     2221 cacgcctggt ggccgcaggg atacgcgatc gacgccctga ccgagcgatt cggcggactg
     2281 gtccgtcatc acccggacgg tcgcacgcag cggatgggct tcgactcgcg tggcgtaatc
     2341 agcaatgcct gggttgcccg cggcgtggcc gagcagatga cggttcgcca gctgcccgca
     2401 gcacgccact ggtggaatca cccgaccggc gacggtggcc aactcgacct gatcaccttc
     2461 gccaaccgct ggctcctgtt acggccagac cggaacatag cctggcgctg ggcagactga
     2521 gttgagggtt aagagttaag ggaacgaaaa cctaaaaccc aagacctaaa acctaagacc
     2581 ccacgaacgg ccggaccccg ggcgctgcca ggattcccgt tcgaccggtg ttcggcacgc
     2641 gatacaggtg gccctcgagg gtggtcacgt acagctcgtc gaggttcggg ccgccgaaca
     2701 cgcagttggt cggcgcgccg tccggcagcc ggtgctcttc gatcaccgtg ccgtcctggg
     2761 cgaagatcgc gatccgcgga ccgggccccg attgcgccca gccgcacgtc gcgacgatga
     2821 acccctcgct gtcccagcac atcccgtcga tcccgcgggc atcgccgaag tcgtgcagta
     2881 ccttcggcga ctccagcgag ccgtcgaggt ggattggata ggcgcggagc tggcgatcgc
     2941 cggagtagtc gctctgggcg acgaagagcg tccgctcatc cggagcgagc agcagcccgt
     3001 tcggccgcgt ggtatcgctg gtcaaccgct cgatcggcca gggcagcgca tcgtcctcgg
     3061 tcggtgtcag gcgatagacc gagtcgtggg tcagctcgcg accggtctga tcgccgtagc
     3121 gcggatcggt gaaccagatt cggccctggc tatcgaggac gaggtcgttc gggctgttca
     3181 accgcatctc attgaacttg gcgcgaagga tcgtcttctc gcccctggtg ttgtaccgga
     3241 cgatcgctcc tgtcgcctgc tcgcaggcgt agagcatgcc gtccaacccg aagatcaggc
     3301 cgttcgtggc gttggtgtag tggtaaacgg tcgagatctc gccggatgcc cggtcatagc
     3361 gccggatctc gttggcgacg atgctcgaga agaagagccc ctcgccgtcc caggcaggcc
     3421 cttccgtgat cgtctccggt cccgccagca gctcccacgt ccattcagcc atctcggtta
     3481 tgccccttcc gctacggggg caatttaaca aaggattggt tgagggttga gggtggaggg
     3541 ttaagggaac gaaaacggaa aacaaaagac gagagaccaa agaccaaaga ctggagaccc
     3601 catccatgct cgacgatacc gtcatcatcg ctcttgttcc tggctgcgtg gaggtggcga
     3661 agcggtccgg tttgccggcc cgtttcgcac cgatcgccgc catcctgacc gcgctcgtgc
     3721 tcgtctcgct gtcccaggtt gccgatgcgc ccggcccaat cacactggcg ctgatcgccc
     3781 ggctgctgct gaccggcctg atcgacggcc ttgccgcgat cggcctctac cgcctcgtcc
     3841 cgaagggagc catgacggcc acctgacata gtcctcggcg gctcgctcgg ccgctcgtcc
     3901 ccactacacc agaggaccga cgcggggccg gcaaagcagg tcttggatgt gggctgtatc
     3961 ctaggttacg aatcccgcat ttcgttgtcg caggagcgtc tcccgtggcc cgcaaaggca
     4021 agaacgtacg aaacacatca actccagtgg ccgcggtcga tgttgggtcc aactcgatca
     4081 agatgaccgt cgcccggctg cggcgcggca cgctcgacat cctggccgag gaatctgaaa
     4141 cggttcgtct cggcgccggt ctcgattcga ccaggacgct tgcgcaggat cgaatcgccg
     4201 ccgccatcga gacgctctcc cgtttcgccg ggattgcccg tgggcacggc gcaacccggc
     4261 ttctcggcgt cgccaccgag gccacgcgcg ccgcaaccaa cggcgaggtg ttcctgaaca
     4321 gggcccgcga ggagaccggc tgggatctcc gcgtcatctc gggcgacgaa gaagctgggc
     4381 tgacgtttcg cggtctcgcg gtgattgggg atatgaccgg ctcggtcgtg gtcgccgata
     4441 tcggcggcgg cagcaccgag ctgatcgccg cggagtcgag tgcggtcgcc atgtttcgct
     4501 cgatcccgct tggctccggc cgcctgacgg atcggttgat cacgcacgac ccgccggttc
     4561 ccgcggagat cgaggcctgc gctgatgccg ccaccgggca gctcggcgca gtcccgttcc
     4621 cggtgtccaa tccagaccag atgatcctcc tcgggggaac cgcggaggcg ttggcgcgtc
     4681 tcgtccccga tcgcgaccac atctcgacta ccgatctgga aaccgcgctc tgtcgcctga
     4741 tggaggagcc cgccgcgccg cttgccgaat cgaccggcat cgcggaggcc cgggcgcggg
     4801 tgctcccggc aggggtggcg atcatgcggg caatggcgca gatgctccac ccggcgaaca
     4861 tgcgcctggc cgccagcggg atccgcaccg gtctgatcct taccgccctg gacgaggcga
     4921 gcaggatccc ggtcgatccc tgtgccgcca tgagtgggcg aatcgcttcg ttgtgggagg
     4981 aagtcgccgc gacaatgccg gcggcgatcg ccggcgagga ccccgaaggt gtgcacaaga
     5041 tgcgggtcgc ctcccgccgg ttgcgggcgg ccatggatat cgcgacgcca tgcttcccca
     5101 cgaagtggta ccggagcctg caccggaccg cgcggcaggt cacgcgctcg gccggcgcgg
     5161 ttcgcgatcg cgaagtgctg ctgctcttcc tgaaatcgga gcgcaagaag aagctcacgc
     5221 ccgagatgcg cgccgcgatc gaccactgga gtgcggagct ctccgtcgag cgggagctcg
     5281 cccgggtcac gatgttggac ttcctcgccg agttcggggc caatgacgtg ctggccaagg
     5341 cgagacgacg attcttggcc cggggcgctc catcagggaa atcgtgccgc aaatgccgtg
     5401 gcaaggcgga tgtgatgctg atcgatccgg agcgatcgtg gacggacaac gcgagtcgag
     5461 tcctggagcc ccggattcac gacctgttct cgttcggcga tgcgatcgct gacccggagg
     5521 cgatcgaagc ccatcacagc gcgaggatcg ccggcaagcg attgcggtac acgctcgagc
     5581 tcttcgcaga tgtcttcggg aagccgggca cgcatgccct cgacgacctt accgacctcc
     5641 aggattccct cggcaagatc cacgacaccg atgtccgtat tgccctgatc gcagacgaga
     5701 tcaagatcca ggccgaggga catgatcccg atctcaccat ccaggcgggt ctcgagcagt
     5761 tggctgagcg ctttcagcgt tcccgccacc gggaacatgc caccctggcg cgcatgtggc
     5821 agagggacgc agccaacttc gagcaggagc tcatc
//
LOCUS       DATAOZ010000035         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8963566, whole genome shotgun sequence.
ACCESSION   DATAOZ010000035 DATAOZ010000000
VERSION     DATAOZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8963566"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..332
                     /locus_tag="VHR64_00970"
     CDS             114..332
                     /locus_tag="VHR64_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301426.1"
                     /translation="MNRVKSAYLRHGPYPALIGSFVPTKKNLPARGFYPRQGFALVEV
                     RASGEQLYRVTRDNCPLLDCRHIAVEES"
     gene            329..559
                     /locus_tag="VHR64_00975"
     CDS             329..559
                     /locus_tag="VHR64_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3301427.1"
                     /translation="MNQRLTQVLCDVLDLDPARVGPDLMREEVDQWDSLNHLKVVTAV
                     EEAFDLKLTMQEIESVDGIARLIDLLEAHDRL"
     gene            565..1089
                     /locus_tag="VHR64_00980"
     CDS             565..1089
                     /locus_tag="VHR64_00980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX3301428.1"
                     /translation="MTAVQVLRRFLPSVLITLGYWLKFGCFVSPRAEVDFTSNVRIGR
                     RTKISSFCKIKAAVGPLTIGADTSIGAGCFIGAGGAATTIGDDCLISPHVTILASNYR
                     YERLDVPIQEQGQTSRGVRIADNVWIGAGVVVLDGSDIGEGAIIVPNSVVSSRVPANA
                     VAHGNPAKVVFTRR"
BASE COUNT          284 a          485 c          503 g          314 t
ORIGIN      
        1 aacatcaacc ttgatagttt ggtattcctg gacgaccgcg agggcgacga gccggtggtc
       61 gacagccgat cctgagttgc cgtgccttgg gccggaagat cgaatccgcg atcatgaacc
      121 gggtcaaatc ggcctatctg cggcacggcc cctatcctgc cctgatcggc agcttcgtcc
      181 cgaccaagaa aaatcttccg gcacgagggt tctatccgcg ccagggtttc gctctggtcg
      241 aggttcgtgc ctccggcgag cagctttatc gcgtaacgcg cgataattgc cctttgctcg
      301 actgtcgcca catcgccgtc gaggaatcgt gaaccagcgt ctgacccagg ttctttgcga
      361 cgtgctcgat ctcgacccgg ctagggtcgg ccccgacctt atgcgtgaag aggttgatca
      421 atgggattcg ttgaatcacc ttaaggtggt cacggcggtc gaggaggcgt tcgacctcaa
      481 gttgacgatg caagaaatcg aatcggttga cgggattgcg cgtctgatag acctgctcga
      541 agcccacgac cggctgtagt cgccgtgacc gccgttcagg tcctgcgacg gttcctgcca
      601 tcggtgctga tcacgctggg ctactggctg aagttcggct gcttcgtcag cccgcgggcg
      661 gaggtggact tcacctcgaa cgtgcgtatc ggccggcgca ccaagatcag ctcgttctgc
      721 aagatcaagg cggccgtcgg ccccctcacg atcggcgccg acacgtcgat cggcgctggc
      781 tgcttcatcg gcgcgggtgg cgcggcgacg acgatcggcg atgactgcct gatcagcccg
      841 cacgtcacga tcctggcgtc gaactatcgt tacgagcggc tcgacgtgcc gatccaggag
      901 caaggccaga cctcgcgcgg cgtgcggatc gccgacaacg tctggatcgg cgccggagtc
      961 gtggtcctgg acggcagcga catcggcgaa ggcgcgatca tcgtgcccaa ttccgtggtc
     1021 tcctcgcggg tgccggcgaa cgcggtcgca catggcaacc cggccaaggt ggtcttcacc
     1081 cgccgttgag ccgtaacctg gtctggttga gaccgaccac tacccattgg tctgtttcgg
     1141 cgcgctcgcg tctggtagga catctcgcgg atcggcagca ccgacagggt cgcagtactc
     1201 cgatgaggag agcgggattg gctgagcggg ttctggtcac cggcggcgcc ggatttctcg
     1261 gttcgcacct ttgcgagtgg ctggtgaggc ggggccacga cgtcctgtgc gtcgacaatt
     1321 tttacaccgg aaataagatg aacctgcggg cgccgcgggg catccggact tcgagatcat
     1381 gcgccacgac atcacctttc cattgtatgt cgagatcgat cagatcttca atctggcgtg
     1441 cccggcggcc ccgatctatt accagttcga tccagtcaga cgaccaagac cagcgtgcat
     1501 ggcgcgatca acctgctggg gctggccaag cggctgaggg cgaagatcct gcaagcctcg
     1561 accagcgaag tgtatggcga ccctac
//
LOCUS       DATAOZ010000036         7451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_20582, whole genome shotgun sequence.
ACCESSION   DATAOZ010000036 DATAOZ010000000
VERSION     DATAOZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20582"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /gene="cgtA"
                     /locus_tag="VHR64_00985"
     CDS             <1..376
                     /gene="cgtA"
                     /locus_tag="VHR64_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEX3301429.1"
                     /translation="IRTYSLADLDEKAFTVRRTGPERFEVTGVQIERTTRMTNFDLQE
                     ASDRFQSILERSGITKELNRQGIEQGDTVVIGDRELIWGIQEELEPVGSKRRTARERY
                     MNRKSRVVDTEPTFTEDESADT"
     gene            complement(561..1313)
                     /locus_tag="VHR64_00990"
     CDS             complement(561..1313)
                     /locus_tag="VHR64_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX3301430.1"
                     /translation="MTTRDPLSKEAIEHGTIRGRPRRILRQPFLWLFFLLFRMRLKHV
                     ERVPKDGGVLVVANHIHNADPILLNAAYPRPIHFMAKKEAFEVPVIPWALRWVGAFPV
                     DRGKSDRWAIKRALAALNGGIAVAMFPEGTRSRVFALQKGHPGAGMLALNADVLVQPV
                     AITGTERLPFNGSKGKATGELERDPGHSGAQILFGEPFRIPREIEGRRVTADEATEIM
                     MLEIARMLPEAYRGVYAEPLKRSAEGKAIRKS"
     gene            complement(1310..1987)
                     /gene="cmk"
                     /locus_tag="VHR64_00995"
     CDS             complement(1310..1987)
                     /gene="cmk"
                     /locus_tag="VHR64_00995"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX3301431.1"
                     /translation="MVAIDGPAASGKTTVAAHLADRLGAVFLDTGLLYRAATLLALRL
                     GLSAADEVRLSMLIDAGAVSIRPATVPDGRRLDVLLNGEDVTTMLRAPEIDANVSAIS
                     ALPAIRAALMPIQRTFAKDRRVIMVGRDIASVVFPDAGVKIYLDASLGERARRRWLEQ
                     VTNEPMLTQAEVEADLRRRDRIDSTRETAPLQVAEDAVVVDTDFKSIDQVVDEVAAIV
                     ERAWDAA"
     gene            complement(1902..2987)
                     /locus_tag="VHR64_01000"
     CDS             complement(1902..2987)
                     /locus_tag="VHR64_01000"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX3301432.1"
                     /translation="MERLQRILASRGVASRRAAEELIREGRVSVNGVVVTALGTKADP
                     QRDEIRVDKRIIRPQALRYLILNKPSGYITTVKDERDRWTVMDLINVQERVFPVGRLD
                     RDTEGLLLFTNDGDVANRVMHPRYGLAKEYQVLTRTRPNPQQLERIRRGIVIEGRRIV
                     PQEVRLLRESADGVIITITVHEGIFHLVRRIMDVAGIEVERLRRARIGPLSIAGLRIG
                     EWRDLTPGEQSTLFEALHLTEEEAAEKSIERPIRIRASGAQPPANPLWKRPKRAGEEP
                     EPGPNVPRYRKPSRTEREAAERGRPATDSRERTEQERDDRPQRERSRRRTERPERPER
                     GRHRRSGGEREDNRCRSSGRPPRRRLP"
     gene            complement(3015..4160)
                     /locus_tag="VHR64_01005"
     CDS             complement(3015..4160)
                     /locus_tag="VHR64_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3301433.1"
                     /translation="MRARAAVLVCLLLSLVAPFAPAGVSAQELAATPSVSAHAAYAFD
                     ATANVELLSENSTERRAPASTVKIVTAMVVLDHAQLDTQVVFEAADAATEEESRMGLE
                     AGDTVTVEQLLVGLMLPSGNDAARTLARHVGTILLDGQPGDPIERFVEEMNAKVAEAG
                     LTNTHFTSPDGLSDDDNQYTTAYDLAHLGAMAMGYKQIAQTVSQSKAEITSVGPEARV
                     YSLQNTNKFLEDSGTQYATDGVIGLKSGSTTKAGANLVLAKRERGGNLLIAVVLGSEL
                     SYDAQGLIAVDGRWDDMRAVLDSVEGTFAWINPESSNDMPGLVDEMSAWQVSLKDDSG
                     ILVDRSIRKEIRYQLELLPQGPAKSDAGRVLFFAGADQIAERPLIFR"
     gene            4354..5103
                     /locus_tag="VHR64_01010"
     CDS             4354..5103
                     /locus_tag="VHR64_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3301434.1"
                     /translation="MRIAAISDLHIWGPISKKLITEFDELHERADVLVIAGDITNNGL
                     LVQAERAATLLERARIPIVAVLGNHDRRLPRRRGYTTTLEAAGVTFLDGTTAEFGSNI
                     RVGFAGVQGSGGGFWPQEGPDTIPRRAAQALAVRSRREAARLDAALTRLTTDLKIVVT
                     HVSPTMTTLGNEPRIKFFLLGNSELARVIDRHDVDLVIHGHVHRGNTHGVTPGGTLVR
                     NTAKNVCREMQYFDAAQLLEARQAPAIEAIA"
     gene            5100..6116
                     /locus_tag="VHR64_01015"
     CDS             5100..6116
                     /locus_tag="VHR64_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3301435.1"
                     /translation="MTSTRSLTRTSSATHRLDLLTNPLLPSLRVYEALSSEIQLRRPA
                     ALLEGALVERIAAMESVPTDWIAIGSGIDDLIVAILRSYGNPENLVLFPPTDLSVARH
                     AARLGMTPVLIPRSLQFTSDLDRAGLPAFPPNALTIVQHPNNPTGTPTTVHDAVRMIR
                     RSRLLVVDERHAGYGARSLLPLVREFDRVVVLRTFETWAGLASLPISYAIGSPDVIAA
                     INVALLLRVGAGPLIAAHASLDDARSVNLAVARVKDEKARMYRMLRKLNMVRPMPSWA
                     NFVLVRVERGDPSLYPDELLKREIRIHVPEQPELSGYFRLSAVSHEATAALRAALIEI
                     GQIV"
     gene            6150..6827
                     /locus_tag="VHR64_01020"
     CDS             6150..6827
                     /locus_tag="VHR64_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301436.1"
                     /translation="MAGEVRVALEIGPKGKKVVAVAQDWPGLSRGARTAEDAIERLRA
                     YIPRYAPVATAAGMAAALAPDPGIVVVEQYPGVGSTDFWGISFAFSHFDQQAMPQDEL
                     DRELALMRACWTYFDDVRSRVTAEMARGPRGGGRNRDQIVRHTIVSELDMAKKVGLRW
                     MEERHPSETESAEYRDAYCAAIRAFHAEGKSARKWPLRYLIRHTAFHTLDHAWEMEDK
                     DLSAGRA"
     gene            complement(6848..>7451)
                     /gene="rph"
                     /locus_tag="VHR64_01025"
     CDS             complement(6848..>7451)
                     /gene="rph"
                     /locus_tag="VHR64_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX3301437.1"
                     /translation="TVEERVPKWITQRGRGWVTAEYGMLPRATKERTRREAAEGKQGG
                     RTVEIQRLIGRSLRAIIELDKLGERQIILDCDVLSADGGTRCASITGAYVAMALAIRK
                     LIEQKKLREDPLLGQVAAISAGVVKGVPMLDLDYSEDAGADVDFNVVMTDAHAFVEIQ
                     GTAEGQPFLPATLTEILVLASTGLDQLFAAQREALSRATS"
BASE COUNT         1269 a         2539 c         2358 g         1285 t
ORIGIN      
        1 aattcgtacg tactcgctgg ccgatctcga cgagaaggcg tttaccgtgc gacggaccgg
       61 tcccgaacgc ttcgaggtga ccggagtcca gatcgagcgg accaccagga tgaccaactt
      121 cgatttgcag gaggcatctg accgcttcca gtcgattctc gagcgcagcg gcatcaccaa
      181 agaactgaat cggcagggca tcgagcaggg cgatacggtc gtgatcggcg accgggagct
      241 gatctggggc atccaggaag agctggagcc ggtcggctcg aagcggcgca cggcccgcga
      301 gcgctatatg aaccgcaagt cccgggttgt cgacactgaa ccgacgttta ctgaggatga
      361 atccgccgat acctgacgct gcgtcgagcc gccgggcggc accccaacac gtggcgcgac
      421 tcggtcacga cctacccaac acgtaccggg gggccgccgt gccccgctgt ggccgcgggc
      481 cacgacggtc tcgccgggag atcggatgcc ggaaggcggc gggtctttcg gcagcacata
      541 cggggcgcgg cgaccccccg ctacgacttc cggatggctt tgccttccgc gcttcgcttg
      601 agtggttcgg catacacccc gcggtacgcc tccgggagca tccgggcaat ctccagcatc
      661 atgatctcgg tcgcttcgtc ggcggtcacc cggcgacctt cgatttcgcg cgggatccgg
      721 aacggctccc cgaacaggat ttgcgcgccg gagtgaccgg gatcgcgctc cagctcgccc
      781 gtcgccttcc ccttcgagcc attgaacggc aaacgctcgg ttcccgtgat cgcgaccggc
      841 tggaccagca catccgcatt cagcgccagc atgcccgcac ccggatgccc cttctggagc
      901 gcgaagacgc ggctccgcgt cccctccggg aacatcgcga ccgcgatacc gccattgagc
      961 gcggcaagtg cccgcttgat tgcccaacga tcgctcttac cccggtcgac cgggaacgcg
     1021 ccgacccaac gcaacgccca tggaatgacc gggacctcga acgcctcctt cttggccatg
     1081 aagtggatcg ggcgcggata ggcggcgttg agcagaatcg gatcggcgtt gtggatgtgg
     1141 tttgcgacca cgagcacacc gccgtccttc ggcacgcgct cgacgtgctt gagtcgcatc
     1201 cggaacaaca ggaagaagag ccagaggaac ggctggcgca ggattcgacg cggtcgccct
     1261 cggatcgtgc cgtgctcaat cgcctccttg ctgagcggat cacgtgtcgt catgccgcgt
     1321 cccacgcccg ctcgacgatc gcggcgacct cgtcgacaac ctggtcgatc gacttgaaat
     1381 cggtgtccac aaccacggcg tcttccgcga cctgcagcgg cgcggtctcc cgcgtgctgt
     1441 cgatccgatc gcggcggcgt agatctgctt cgacctcagc ctgggtcagc atcggctcat
     1501 tcgtgacctg ctccagccag cgcctgcggg cccgctcccc gagacttgcg tcgagataga
     1561 tcttgactcc ggcatcaggg aacacgaccg aggcaatgtc gcgaccgacc atgatcacgc
     1621 gccgatcctt ggcgaatgtg cgctggatcg gcatgagcgc ggcccggatc gccggcaagg
     1681 ccgaaatcgc cgaaacattg gcatcgatct ccggcgcgcg aagcatcgtc gttacgtcct
     1741 cgccgttcag caggacatcg aggcggcgac catccgggac tgtggccggc cgaatcgaaa
     1801 ccgcaccagc atcgatcagc atcgaaagcc gcacctcgtc ggccgcgctc agccccaggc
     1861 gcagcgcaag cagggtggcc gcccggtaga gcaggccggt gtcaaggaag acggcgccga
     1921 ggcggtcggc cagatgagcg gcaacggttg tcttcccgct cgccgccgga ccgtcgatgg
     1981 cgaccacgtt ccggccgctc tggtcgttcg gtacggcgcc ggctgcgctc tcgctgcggt
     2041 ctgtcatctc gctcctgttc cgtccgctcc cggctatcag tagccggtcg accgcgctcg
     2101 gcggcctcgc gctcagtccg gctgggcttt cggtatcgtg gcacgttcgg tcccggctcg
     2161 ggctcttcac cagcgcgctt tggccgcttc cagagcggat tcgctggcgg ctgggcgcca
     2221 ctcgcccgga tcctgattgg ccgttcgatg ctcttctcgg cggcctcctc ctcagtcagg
     2281 tggagcgcct cgaagagggt cgattgctcg cccggcgtca agtcccgcca ctcgccgatc
     2341 cgcaacccgg cgatcgagag cgggccgatc cgggcccggc gcaaccgctc gacctcgatc
     2401 ccggccacgt ccatgatccg ccgcacgagg tgaaaaatac cctcgtggac cgtgatggtg
     2461 atgatcacac catcggccga ctcacgcagg agccgaacct cctgcggcac gatccgccgt
     2521 ccttcgatca cgataccgcg ccggatccgc tccaactgct gcgggttcgg ccgggttcgg
     2581 gtcaggacct ggtattcctt ggccagccca tagcgcgggt gcatgacgcg gttcgccacg
     2641 tcgccgtcgt tcgtgaagag cagcaatccc tcggtgtcgc ggtcgagccg gccgaccggg
     2701 aagacgcgct cctgcacgtt gatcaggtcc atcacggtcc agcgatcgcg ctcgtccttg
     2761 acggtcgtga tgtagccgct tggcttgttc aggatcaggt agcgcagcgc ctgcggacga
     2821 atgatccgct tgtcgacccg aatctcatcc cgctgcggat cagccttggt gccaagcgcg
     2881 gtcacgacca cgccgttgac cgaaacccgg ccctcgcgaa tcaattcttc ggctgcccgg
     2941 cgcgatgcga ccccgcgcga tgcgaggatg cgctgcaagc gttccacttg gatgcctcgt
     3001 gatatgtcgt gtagctagcg gaagatcagc ggacgctctg cgatctggtc cgctccggcg
     3061 aagaatagca cgcggccagc atcgctcttc gccggcccct gtggcagaag ctcgagttgg
     3121 taccggatct ccttgcggat cgatcggtcg accaggatac cggaatcatc cttgagcgag
     3181 acctgccagg cggacatctc gtccaccagc cccggcatgt cattgctgga ctccggattg
     3241 atccaggcaa acgtgccctc gaccgaatca aggactgccc gcatgtcgtc ccagcgccca
     3301 tcgacagcga tgagaccctg cgcgtcgtag ctcagctcgc taccgagcac aaccgcgatc
     3361 agcaaattgc cgccccgctc gcgcttcgca agcaccaggt tggcgccggc cttggtggtc
     3421 gagcctgact tgagtccgat cacgccatcg gtcgcgtact gcgtcccgga atcctccagg
     3481 aacttgttcg tgttctggag cgagtagacc cgcgcctcgg gaccgaccga ggtgatctcc
     3541 gccttggact gggagacggt ctgggcgatc tgcttgtagc ccatcgccat tgcgccgaga
     3601 tgcgcgagat cgtaggcggt cgtgtactgg ttatcgtcgt cgctgagacc atctggcgag
     3661 gtgaagtgcg tattggtcag tcccgcctcg gcaaccttgg cattcatctc ctcgacgaac
     3721 cgctcgatcg gatcacccgg ctgaccgtcg agcagaatcg tcccaacgtg ccgggccagc
     3781 gtgcgcgcgg cgtcgttgcc ggacggcaac atcaggccga cgagtaattg ctcgaccgtg
     3841 accgtatcgc cggcctcgag ccccatccgc gactcctctt cggtcgcggc atcagccgcc
     3901 tcgaagacga cctgcgtatc gagctgggcg tgatcgagca cgaccatcgc ggtcacgatc
     3961 ttgaccgtgc tggccggcgc gcgccgctcg gtgctgttct cggagagcag ctcgacattg
     4021 gcggtcgcgt caaaggcgta ggcggcgtga gcggagacgc tcggggtcgc cgccagctcc
     4081 tgagccgaga cgccggccgg ggcaaacggc gcaaccaggg agagcagcaa gcacaccaat
     4141 acggcggctc gcgcgcgcac tattcccatc cgccgactga cctcccgaaa cgagctgtac
     4201 gtacgcccat aagtataatc gctgccccgt cgcgggacct ccgtctgacg ccgaggaacg
     4261 ccgatcgctc aagcggtggg gtaaactcgg agtcgcgcaa tggaatactt ctgcgcgggt
     4321 ttcgcgggaa cacagacaac agggatctgg ctcgtgcgca tcgcagcgat cagtgatctc
     4381 catatctggg gaccgatctc aaaaaaattg atcaccgagt tcgacgagct tcatgagcgc
     4441 gccgatgtgc tcgtgatcgc tggagatatt accaataatg gcttgttggt tcaggctgag
     4501 cgcgccgcga cactgctgga gcgcgcccga atcccgattg tggcggtcct cggcaaccat
     4561 gatcggcgac ttccccgccg ccgtggctac acgaccacgc tggaagccgc cggcgtgacc
     4621 ttcctcgacg ggacgacggc cgagttcggg tccaacatcc gcgtcggctt tgccggggtg
     4681 cagggctcgg gcggcggatt ctggccgcag gagggaccgg atacgatccc gcgccgggcc
     4741 gcgcaggcac tggctgtgcg gagccgccgc gaggctgccc ggctcgatgc ggcgctcacc
     4801 cggctcacga cggacctcaa gatcgtcgtc actcacgtct caccgaccat gaccacgctc
     4861 ggcaacgagc cgcggatcaa gttcttcctg cttggcaaca gcgagctggc ccgcgtgatc
     4921 gaccgtcacg acgtcgatct cgtcatccac ggccatgtcc atcgcggcaa cacccatggg
     4981 gtcaccccgg gcggcacgct ggtccgcaac acggccaaga acgtatgccg cgagatgcaa
     5041 tatttcgacg cggcccagct gctcgaggcg cgtcaggcgc ctgcgatcga ggcgatcgcg
     5101 tgacttcgac gcgctccctg accagaacca gctcggcgac gcaccggctc gacctcctga
     5161 ccaatcccct cctccccagc ttgcgggttt acgaggcgct gtccagcgag atccagctcc
     5221 ggcgtcccgc cgctcttctc gagggcgcgc tggtcgagcg gatcgcggcg atggaatcgg
     5281 tcccgacaga ctggatcgcg atcggctcag gcatcgacga cctgatcgtg gccatcctgc
     5341 gctcctacgg gaaccctgag aacctcgtgc tcttcccgcc gacggacctc tcggtcgccc
     5401 ggcatgccgc ccggctcggc atgacgccgg tcctcatacc acgttcgctt caattcacgt
     5461 ccgatctgga ccgcgccggc ttgcccgcct tcccgccgaa cgcgctgacg attgtccagc
     5521 acccgaacaa ccccaccggc acgccaacga cggtccacga cgcggtacgg atgatccgcc
     5581 gctcgcgctt gctggttgtc gacgagcgcc acgccgggta cggcgcgcga tcgctcctgc
     5641 cgctggtgcg cgagttcgac cgcgtcgtcg tcctgcgcac gtttgagacc tgggcagggc
     5701 tcgcctcgct gccgatctcg tacgcgatcg gctcgccgga cgtgatcgca gcgatcaatg
     5761 tcgccttgct gctgcgggtg ggcgccggac cactgatcgc cgcccacgcc tcgctcgacg
     5821 acgcgcggtc agtcaacctg gcggtggcgc gagtcaagga cgagaaggcg cggatgtacc
     5881 gaatgctgcg caagctgaac atggtccggc cgatgccatc gtgggcgaac ttcgtgctgg
     5941 tcagggtcga gcgcggcgat ccatcgctct atcccgatga gttgttgaag cgcgaaatcc
     6001 gcatccacgt gcccgagcaa cccgagcttt ccggttactt ccggctcagc gctgtctctc
     6061 acgaagccac cgccgccctc cgcgccgccc tcattgaaat cggccagatt gtctgatccc
     6121 aagttctcaa caatcagcgt ggaggatcaa tggctggtga agttcgggta gcgctcgaga
     6181 ttgggccgaa gggcaagaag gtcgtcgcgg tggcccagga ctggcccggc ctctcgcgtg
     6241 gagccaggac cgcagaagat gctattgagc ggctccgggc gtacattcct cgatatgcac
     6301 cggtcgcaac ggcggcaggc atggcggcag cattagcgcc cgaccccggc atcgtcgtag
     6361 tcgaacagta cccaggcgtc ggatcgaccg acttctgggg tatctccttc gccttctcgc
     6421 acttcgacca gcaggccatg ccgcaggatg agctggatcg cgagctcgct ttgatgcgcg
     6481 cgtgctggac ctacttcgac gacgtgcgct ctcgggtcac ggccgagatg gcgcggggcc
     6541 cgcgaggagg cggccggaac agggatcaaa tcgtccggca cacgatcgtc tcggagctgg
     6601 atatggcgaa gaaggtcgga ctgcgctgga tggaggagag gcacccgtcc gagacggagt
     6661 cggccgagta ccgggacgcc tattgcgccg caatcagggc attccacgcg gagggaaagt
     6721 cggcccggaa gtggccgctc cgatatctca tccggcacac cgcgttccat accctggacc
     6781 acgcgtggga gatggaagac aaggacctgt ccgccggccg ggcgtgagcg ggaataagca
     6841 gggccaatca gctcgtggcg cgagaaagcg cctcacgctg tgccgcgaag agctgatcaa
     6901 gaccagtgct ggcgaggacc agaatttcgg tcagggtagc gggcagaaag ggctggccct
     6961 cggccgtgcc ctggatctcg acgaaggcgt gcgcgtcggt catgaccacg ttgaaatcga
     7021 cgtccgcgcc ggcatcctcg ctgtaatcga ggtcgagcat cggcacgccc ttgacgacgc
     7081 ccgcgctgat cgccgcgacc tggccaagca gcggatcctc ccgcagcttc ttctgctcga
     7141 tcaacttgcg gatcgcgagc gccatcgcta cgtacgcccc ggtgatcgac gcgcaacggg
     7201 tgccgccgtc agcactcaga acgtcgcagt cgaggatgat ctggcgctct ccgagcttgt
     7261 cgagttcgat gatcgcccgt agcgaccggc cgatcagccg ctggatctca accgtgcggc
     7321 cgccttgctt gccctcggcc gcttcccgcc gcgtccgctc tttggtggcg cggggtaaca
     7381 tcccgtactc ggcggtgacc cagcctcgac cgcgctgcgt gatccacttc ggcacacgct
     7441 cctcgacggt c
//
LOCUS       DATAOZ010000037         5250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6333902, whole genome shotgun sequence.
ACCESSION   DATAOZ010000037 DATAOZ010000000
VERSION     DATAOZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6333902"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VHR64_01030"
     CDS             <1..483
                     /locus_tag="VHR64_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX3301438.1"
                     /translation="TMPYWHDDIGAHFFEAISEADALLLGRKTWQIHGGAFETMDGNP
                     FADVLNGLQKYVVSTTLSDASAWRNSTLISDNVVEEVRSLKEQPGKTIYVDGSSKLVH
                     TLAENDLVDEFSLLVYPISLGSGKKVFPDGTRVNLRLIETHPYPSGVVLMRYEPDHSA
                     "
     gene            complement(586..1266)
                     /locus_tag="VHR64_01035"
     CDS             complement(586..1266)
                     /locus_tag="VHR64_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301439.1"
                     /translation="MSTMRPNPASLRRVALLLVVLALLGSKSLLAARAEDQGEKPYND
                     SVNQQALCELLGGHATVEQTMNAAGLYVVTVTCHGGLLDGLACQNGFPGSPEPNCWWA
                     WRIQPTDDLTPKVTESIEPLEEPTVAAPQIVDQTDVGDVTQTAPGNPEPALTPTSEAT
                     DVSGDSGEPGDTTPVVDNGAVGDGTIDTQAPGAEPPVIIDPGPGGQTLPGSNGEVPMV
                     LPVIAPGQ"
     gene            complement(1528..2046)
                     /locus_tag="VHR64_01040"
     CDS             complement(1528..2046)
                     /locus_tag="VHR64_01040"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX3301440.1"
                     /translation="METRYLATTDSPAGPLHVAVTGDGQLCGIWFGVVTSGVEVEASL
                     HRRGRAPVWDSDRVAHVTAQIAEYGDRRRTSFDLKLAIEGGEWEQQVWQALLTIPYGE
                     TRSYGQIATQLGDPSKARAVGWANAANPIPLVIPCHRVIGANRNLTGFGGGIDAKIQL
                     LAHEGAMLPSLA"
     gene            complement(2424..2606)
                     /locus_tag="VHR64_01045"
     CDS             complement(2424..2606)
                     /locus_tag="VHR64_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301441.1"
                     /translation="MKASIRTFGQFLNPFQHGDRRITGYYRGVIEPGSGYPTYDEARR
                     DLARRDRETFVPPVWH"
     gene            complement(2751..2921)
                     /locus_tag="VHR64_01050"
     CDS             complement(2751..2921)
                     /locus_tag="VHR64_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301442.1"
                     /translation="MKTLRIFLNPFRGLPRRNERYYQGVIGVGAGYPTYDEARRDLML
                     RDRAMIPQGGWR"
     gene            3094..3612
                     /locus_tag="VHR64_01055"
     CDS             3094..3612
                     /locus_tag="VHR64_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3301443.1"
                     /translation="MSGVTQIRARFSFLAMCGVIAVVAVACGRASEEDINNALNITPT
                     PTLSAQQVAEQTAAAQSEQSTREAAQAALASPGAEGSAVVDLAAAGNPNTGRIAFLQR
                     CQSCHRPGGGGAGPDLSGPNNPAVALSDEQIVQLLRTGEGHATPPGPLSEVDLNQKQM
                     VDILAFIRQQSK"
     gene            3794..5116
                     /locus_tag="VHR64_01060"
     CDS             3794..5116
                     /locus_tag="VHR64_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301444.1"
                     /translation="MVVLPAISALIAFGCVALIARDALRRPSPDRITWIVAFAMFGIA
                     AAAETIGDTAGWNATLARSYYLTGAVLVVGFLALGQLYLLFPDRIRKVAPGVALLITA
                     ISATTVWSAPIDQSRLSADGWDAIERTTGLSILAISINSIGTLILLGGLGYSAIRFKR
                     SGTHRNRMIGCLLIALGTLAVAMGGTLTRFGSEQYLYIAMSVGIALIFGGYVWMKKGE
                     QARRRGGEQLGRDGAEARGREVGSGEAGLPAYQIHRSAASLGEASFAQPGVSTPGGAA
                     LDLSSTPGGSAQTAGLSLDPSSTPGGAAGTAASGEYPRETRNQKLETSLNPGIAYIEA
                     RLASRSDDELSEECRVWSVPAREIDAFNRAEARRVWVFRNRLSVDGQAAFDARPASLR
                     LQLAEVFFEVMSAEVPLIDRPLVAPIDRLTIRAEADALAARASGGSVD"
BASE COUNT          898 a         1741 c         1643 g          968 t
ORIGIN      
        1 accatgccct actggcacga cgatatcggc gcgcacttct tcgaggcgat cagcgaggcc
       61 gacgccctgc tgctcggccg caagacgtgg cagatccatg gcggcgcgtt cgaaaccatg
      121 gacggaaacc cgttcgccga tgtgctcaac ggcctgcaaa agtacgtcgt ctcgacgacc
      181 ctgtcggatg cctcggcctg gcgcaactcg accctgatct cggacaacgt ggtcgaggag
      241 gtgcgctcgc tgaaggagca gccgggcaag acgatctacg tcgacggcag cagcaagttg
      301 gtccacaccc tggccgagaa cgatctggtc gacgagttca gcctgctggt ctacccgatc
      361 tcgctgggca gcggcaagaa ggtcttcccc gacggcacgc gggtcaatct ccgactgatc
      421 gagacccacc cgtacccatc gggcgtcgtc ctgatgcgct acgaaccgga ccattccgcc
      481 tgacgatttg ccccatagtc ccgcaacgaa cgtctcgtac gccacgcgat ccgggacctg
      541 cgtcgtaacg acgcaggtcc cgggttttgg cttcagtcga tggacttact gccccggcgc
      601 gatgacaggc agcaccatcg gtacctcgcc gttggagccg gggagcgtct gtccgccggg
      661 ccctggatcg atgatcacgg gcggctctgc tcccggcgcc tgggtgtcga tagtcccgtc
      721 cccaaccgcg ccgttatcca caaccggggt ggtgtcgccc ggctcaccgc tgtcgccgct
      781 cacgtcagtc gcctccgaag ttggagtcag cgctggctcg ggattgcccg gagcggtctg
      841 ggtcacgtcg cccacgtccg tctgatcaac gatctggggc gcggccaccg ttggttcttc
      901 gagcggctcg atcgattcgg tcacctttgg cgtgaggtcg tcggtcggct gaattcgcca
      961 cgcccaccaa cagttcggtt ccgggctgcc cggaaagccg ttctggcagg caagcccatc
     1021 cagtaacccg ccgtggcatg taaccgtaac cacgtagaga ccggccgcat tcatggtctg
     1081 ctcgacggtc gcgtggccgc cgagcaactc gcagagtgct tgctgattca cgctgtcgtt
     1141 gtagggcttc tcaccctggt cctcggcgcg cgccgcaagg agcgacttgc tcccgaggag
     1201 ggccaacacg accagtaaca gggcaactcg acgaagggac gctggattgg gtcgcatggt
     1261 gctcatctcg tctctccact ctcatcgtca tttcgacatg gtttgcatct gttgcgatga
     1321 tcgcggagat tcaggtgggc gccatcggtg agatcacgta atcccgtccg acttttcgac
     1381 ctcttgggtg ttcggcatat tgcgcggtcc tgccagaatc ggcgcgggca tttcacccgg
     1441 tagcggccaa tggcttcgcg taatgtcccg tgatccttct gagacgtgcg ggtttgccac
     1501 ggcacacccc tacattcgct tcgctggcta cgccaagctc ggcagcatgg cgccttcgtg
     1561 ggcgaggagc tggatcttgg cgtcgatgcc gccgccgaag ccagtcaggt tccggttagc
     1621 accgatcacg cggtgacagg ggatgaccag cgggatcggg ttggcggcgt tcgcccagcc
     1681 gaccgcgcgg gccttcgacg gatcgccgag ctgtgttgcg atctggccgt aactgcgcgt
     1741 ctcgccgtac ggaatcgtta ggagcgcctg ccagacctgc tgctcccact caccgccttc
     1801 gatcgccagc ttgagatcga acgaggtccg gcgccggtcg ccgtactcgg cgatctgagc
     1861 tgtgacatgc gccacgcggt cggaatccca gaccggcgcc cggccgcggc ggtggagcga
     1921 tgcttcgacc tcgaccccgg acgtcacgac accgaaccag atgccgcaga gctgcccgtc
     1981 accagtcacc gcgacatgca gcggcccagc cggcgaatcc gtggtggcaa ggtatcgggt
     2041 ttccatgaag tcatcctagc gcggaatgga gccgaacgaa tttaagttca cataatgttg
     2101 cacgagatct ctgcgccccg cgatcgcgcg acgtggcacg atgggatgac cggcgaccgg
     2161 gaatgcgtat tcgaacgggt ttccggcgcc gcccggtgcc gacagtccgc gttgggcatc
     2221 actaagccct tcgggactca aaacacagtc gacttcagcc gccttatttt gagtcagccc
     2281 cggccttcag tccggggcga tgccgagggc atcttactga tcaccaacca ccgtttcggc
     2341 gaactgacct cgccctgcgg gcggtcgccg ggcacatagg caccggcgcc gcgtttatct
     2401 cgtatcggcg cgaagatctg taattagtgc cagaccggcg ggacgaatgt ctcgcgatcg
     2461 cggcgggcga gatcccggcg agcctcgtcg taggtggggt aaccggagcc gggctcgatc
     2521 acaccgcggt agtagccggt gatgcggcgg tccccatgct ggaaggggtt caggaactga
     2581 ccgaaggtgc ggatgcttgc tttcatggcg ttttctcctc attaccgagg atagggaacg
     2641 cagccgcaat ccgatatccg aaacgggtca gggcatacaa acaattgacc cctggcggcg
     2701 tgccaggggt caatcgagtt cggggatgcc ggctacgggg ccggccgctg ttaccgccac
     2761 ccgccctgcg ggatcatcgc gcggtcccgg agcatcaggt cgcggcgcgc ctcgtcgtag
     2821 gtcggatacc cggcaccaac gccgatcacg ccctggtaat agcgctcgtt acggcggggc
     2881 agtccccgaa acggattcag aaagatacgc aaagtcttca tcggaactcc tccttcattt
     2941 cctcatcatc atgatatcac aatattggga taatatcatt aacgtattgt gatattcgtc
     3001 ctgattgggc cgtattgccg gatcaagatc agaccgagtg cgcggaggtc ggctgctctg
     3061 gtatgctgcg ccccacgaac agaccagaca caggtgagcg gtgtgactca gatccgggcc
     3121 cggttctctt tccttgcgat gtgtggcgtt attgccgtgg tagccgtggc ctgtgggcgc
     3181 gcgtcggaag aagacatcaa caacgcgctg aacatcacgc cgacgccgac gctctccgct
     3241 cagcaggtcg ccgagcaaac ggccgccgcg caatccgagc aatccacgcg cgaagccgcc
     3301 caggccgcgc tggcaagccc gggcgctgag ggcagtgcgg tcgtcgacct ggccgctgcc
     3361 ggcaacccga acaccggtcg gatcgccttc ctccagcgct gccagagctg ccaccgccca
     3421 ggcggaggcg gcgccggccc ggacctttcc ggacccaaca atccggctgt tgccctttcg
     3481 gacgagcaga tcgtccaact cctgcgcacc ggcgagggtc atgccacccc gcccggcccg
     3541 ctctccgaag tcgacctcaa ccagaagcag atggtcgaca tcctcgcctt catccggcag
     3601 caatcgaagt aaggcgggaa gtcgggaggc gcgaaggcgc ggggttggtc tttcgtctct
     3661 ggtctttcgt tttcggtttt cggttccctc aacacttaac tcttaacgaa cttcccgccc
     3721 tcccgccttc ccgcctccgc gactcagtta caatcgcccc ggttgtgacc gttgctgcgt
     3781 tccgaggctc ttcgtggttg tccttcctgc catctctgcc ctgatcgcgt tcggctgcgt
     3841 cgccctgatc gcgcgcgacg ccctccgccg gccctcgccg gaccgcatca cctggatcgt
     3901 cgccttcgcc atgttcggga tcgccgccgc cgccgaaacg atcggcgata cggccggctg
     3961 gaacgcgacg ctcgcccggt cctattacct gacgggcgcc gtcctggtgg tcggcttcct
     4021 cgccctcggc cagctctacc tgctcttccc ggatcgcatc cggaaggtcg cgccgggcgt
     4081 cgccctcctg atcacggcga tctcggccac cacggtctgg agcgcgccga tcgaccagag
     4141 tcgcctctcc gcggacggct gggatgcgat cgagcggacg accgggctct cgatcctcgc
     4201 gatcagcatc aacagcatcg gcacgctgat tctgctcggc ggcctcggct actcagcgat
     4261 ccgcttcaag cgcagcggca cgcatcgcaa ccggatgatc ggctgcctgc tgatcgcgct
     4321 cggcacgctg gcggtcgcga tgggcggcac gctgacccgc ttcggctcgg agcaatacct
     4381 ctacatcgcc atgtcggtcg ggatcgcgct gatcttcggc gggtatgtat ggatgaaaaa
     4441 aggcgagcag gcgaggaggc gaggaggcga gcagttgggg cgcgatggcg cggaggcgcg
     4501 ggggcgcgag gttgggtcgg gcgaagctgg gctgccggcg taccagatcc atcgctccgc
     4561 agccagcctc ggagaggctt cgtttgctca gcccggggtt tcaaccccgg gtggtgcggc
     4621 gctggacctc tcttcaaccc caggtggctc ggcccagacg gcgggattat cactggaccc
     4681 gtcttcaacc ccgggtggcg ccgcaggaac ggccgcgagt ggcgagtacc cacgagaaac
     4741 tagaaaccag aaactggaaa cctcccttaa cccgggcatt gcctacatag aagcgcggct
     4801 tgcctcccgc tctgacgacg agttgagcga agaatgccgg gtctggagcg tgccggcgcg
     4861 ggagatcgac gccttcaatc gggccgaggc gcggcgggtc tgggtcttcc gcaaccgcct
     4921 ctcggtcgat gggcaggcgg ctttcgacgc ccggccggcc agtctgcgtc tgcaactggc
     4981 tgaggtcttc ttcgaagtca tgtcggcaga ggttccgcta atcgatcgcc cgctggtcgc
     5041 gccgatcgac cgcctcacca tacgcgccga agccgacgct ctggcagccc gcgcctccgg
     5101 cggttcggta gactgatcgg acgggatcgg acatcccggc ggtttcgatc cgcaattcga
     5161 cacgaccgtg gaatagagga ccgcaggtga ctggacaaca ccgccatcca ctatccacta
     5221 accaccaacc accagacgca tgacactgct
//
LOCUS       DATAOZ010000038        11215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4754939, whole genome shotgun sequence.
ACCESSION   DATAOZ010000038 DATAOZ010000000
VERSION     DATAOZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11215
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4754939"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VHR64_01065"
     CDS             <1..1069
                     /locus_tag="VHR64_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX3301445.1"
                     /translation="ALVRETLASIGTDALILTNPANRRWATGFTSAPGVAFSPDVAVI
                     TPSTVELLVAAIHAAWARGEAVVATNVMAHKGQFAPSLIAFLNERGYGSVAIEADALP
                     YPNAREIEAGVTNTVISYLTGLRDTWRASKDPAEIASLERAALVTDQVFTDVASALTP
                     GETESSVARRISARLIENGDGLGFDVIVASGPNAARPHHRPGEREIREGEPIIIDMGA
                     RIDGYCGDLTRTLWLGEPDERFRELYAAVLDAQRATKSVMTAGTPVREVAEAADRALQ
                     QAGFGEYILHSVGHGVGLEIHESPAIRRDLDTVLPLHAVVTVEPGLYVPEWGGIRVED
                     VVVVGESECRTLTTAPKLEFD"
     gene            1098..1658
                     /gene="efp"
                     /locus_tag="VHR64_01070"
     CDS             1098..1658
                     /gene="efp"
                     /locus_tag="VHR64_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX3301446.1"
                     /translation="MIDTGDLRKGMNLDYEGRLVKVIDFAHNKQGRGSAQVRLTLRDI
                     RTGTTTQHSVQAGTKFQPARLERQHVQYLYGDGEQFHFMDTESFEQFALNESAVSDAM
                     KYLKENDVVDLLTYTGEPIDLELPISVTLEVAETDPGLKGDTASGGTKPATMETGVVV
                     SVPLFINIGDRLRIDTRTGEYIERAS"
     gene            1671..2153
                     /locus_tag="VHR64_01075"
     CDS             1671..2153
                     /locus_tag="VHR64_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX3301447.1"
                     /translation="MSETDQEHDEHMSTDLTDLAAFVERMVTVAQRAGMATLTIRTEG
                     LKLELRSGTAVEIVSNHVPQPAALFEAPTPLIPQAEPAGYVITAPMVGTYYRSTSPTD
                     PPLVQPGDRVEVGQLIGIIEAMKIMNEITADRSGTVLDVIAENATAVEFGSPLIRLGP
                     "
     gene            2158..3567
                     /gene="xseA"
                     /locus_tag="VHR64_01080"
     CDS             2158..3567
                     /gene="xseA"
                     /locus_tag="VHR64_01080"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX3301448.1"
                     /translation="MQSQVVTVSQVSDYLRLLTESDAFLSNLLVEGEVTEVYLSKAGH
                     LYFTLSDGQASLKAVAFRNAALRVQHLVTKGAHLTVGGRVTVYSKDQTCQIYVEFVDH
                     AGVGGQALALARLRAQLQADGLFEQERKRPIPVYPRTIGVVTSPTGAVIHDIQTVLRR
                     RFPFVHLIVSPALVQGPGAVDSLLAALDLLIHDGRSEVIIIARGGGSTEDLSAFNDER
                     LARAIYAAPRPIISAIGHETDVSILDEVADLRAPTPTAAAELATPDIADFALDLIDAR
                     ERMARSLTQVLNHQRSDLQQLTQRLDRSSPSLIFAERRTRLAELESRLGLAVHRHLRD
                     AQADCRLISVQLHGAGVRVLDRQRIDLASVQSHMRELPRVQFAERRADLDQSRRRVAT
                     ITQSVLASAYADLDVQKSALVQLNPAAVLNRGFALLTSADGNVIGSVDAVRPGDPIRA
                     VVRDGTIAGNVTGITRRQR"
     gene            3578..3865
                     /gene="xseB"
                     /locus_tag="VHR64_01085"
     CDS             3578..3865
                     /gene="xseB"
                     /locus_tag="VHR64_01085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX3301449.1"
                     /translation="MTESATTAQLLDRCNQLAASDDYEAVRNGLTQVITLLEQPGLGL
                     NDSVRAYEVGRKLAEQCQTLLDAAELRITQLDGAGSDTGTGPLTSRVRLES"
     gene            3867..4895
                     /locus_tag="VHR64_01090"
     CDS             3867..4895
                     /locus_tag="VHR64_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX3301450.1"
                     /translation="MTKLGDILPPGKLPGDLLGRLLERHSVHDERVLIGPGTGRDAAA
                     IQFGDMALVVKTDPITFPTERSALHLVHVNANDIACQGAVPRWLLVTALLPHGSTSAE
                     SASSMFADVNAAARQIGVSVIGGHTEITIGLDRPILVGTMLGEVAVDRLIDPRDASPG
                     DRLLMTKTAGIEGTVILADELRSLLISRGVADNTIDSAATMIDNPGISVLAEARALCM
                     AGALNALHDPTEGGIATAVRELALASGCGVMISRDAIPVAAETAEIATALNIDPLGML
                     ASGSLLAAVPKASLEVAENALATARIPFAWIGKLTPPESGMRIRTGMVESALPEFEVD
                     EVARVLAG"
     gene            4901..5827
                     /locus_tag="VHR64_01095"
     CDS             4901..5827
                     /locus_tag="VHR64_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEX3301451.1"
                     /translation="MGWVLTGSSNADVGFENGSRVLRNGGSAIEAVITTIREVEANPD
                     DHSVGYSGLPNLLGDVELDASIMDGRGLRAGSVGALKNHQDAIDLARCVMEELPHVLI
                     VGDGAERFAGEQQLPKVDLLTDEAKAIWLQRADPTLQRSKYYERMSELVRETTSDPQF
                     PEPPHGTVNVIARDLHGNIACGVSTSGWAWKYPGRLGDSPIIGAGIYADNRFGAAACT
                     GRGEMAQRCLTAHSVVTFMRFGMSLEDALRMAAEDLRSLDDPYASEMNIVAIDVDGTH
                     NAVSTSEGKTYIFQTDAMPSPEYGERIHVPLC"
     gene            complement(5913..7589)
                     /gene="rho"
                     /locus_tag="VHR64_01100"
     CDS             complement(5913..7589)
                     /gene="rho"
                     /locus_tag="VHR64_01100"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX3301452.1"
                     /translation="MSDQEILNPTPAENGPSEVQEPKPRTTRGRRRTAPESQDGTAMA
                     DEGQTATAVIDAPDMTDTSAERSEPREREFSRRHRNGGRNDRDRDRGGRRDRHDRGDR
                     PERGNRAERGERTDYGPREIRSHEMEPQEFTSNGTNPDVPHFTVTELDQMEPEALIEL
                     ARTFEIQGFSRMKRPDLISRLLRSRTEMEGNIFGDGVLEIVDEGYGFLRGSRLLPGSD
                     DIYVSQSQIRRFGLRTGDRISGQVRPPKDNEKFFSLLRVEAVNGVDPEVARRRPNFDS
                     LTPIFPLEMINLETTPNILSTRLLNLVAPVGKGQRGLIVSPPKAGKTLLLKSIANGIT
                     SNDPDMHLMVCLIGERPEEVTDMKRSVEGEVISSTFDEPVEDHTKVAEMALERAKRLV
                     EAGRDVTILLDSITRLARAYNLAVPPSGRTLSGGIDPVALYPPKRFFGAARNIEGGGS
                     LTIVATCLVDTGSRMDDVIYEEFKGTGNMELHLDRKLAERRIYPAIDIQRSGTRREEL
                     LMDDATLRQVWTMRRMVSMLGGSEGTELVLGRLSKTGSNAEFLATLNKDV"
     gene            complement(7943..8935)
                     /gene="glpX"
                     /locus_tag="VHR64_01105"
     CDS             complement(7943..8935)
                     /gene="glpX"
                     /locus_tag="VHR64_01105"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX3301453.1"
                     /translation="MSTASTVDRNLALELARVTEAAAIMAARWMGRGDKNAADQAAVD
                     AMRNVLNSLDMDGIVVIGEGAKDEAPMLYVGEEVGTGAAPHVDIAVDPIDGTRLLSLG
                     MPNAIAVVAVAERGTMLASPDISYMNKIAVGVEAAGFVDVKASVQENLRRVAAAKGVT
                     IGDLTVVVLDRPRHDELIREIRETGARIKMIPDGDVAGAVMAATPGTGVDVLLGIGGA
                     PEAVVAACAIKSLGGEIQCVRYPRNDDERKIAIEQNISLDEVLSTNDLVKSDNVFFAA
                     TGITDGELVRGVQFYENGARTSSLVMRSRTGTIRRIDATHRLDKLRQLSGLPFD"
     gene            complement(8932..>11215)
                     /locus_tag="VHR64_01110"
     CDS             complement(8932..>11215)
                     /locus_tag="VHR64_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX3301454.1"
                     /translation="SQAMHGAASARPPELAFLTNREKPERLKRVKNSDRVEPDEIAAS
                     FDIGGPISKRFDAWEPRRQQREMAVAVAGELRDGGELIAEAGTGTGKSLAYLVPALTN
                     AVMTGEQVVVATNTRVLQDQLASKDAPLAVAAVRESHPGCEPRVQVLKGRSNYLCLRR
                     WFAEMQQKPVPANDEEASFRARANIWLNLTESGEQADLPLERGQAAVFNRVSADGEAC
                     DASRCQFQQRNQCFLYRARRSADAAHVVITNHALLLTDVVQDGEALPEAKHLIIDEAH
                     HLEDQATTSFQVSISSRLISQTLNALAGDNRRGQPGLLVEIGALVHNRKLLTPTHDQT
                     ERVLKSIEAVETAIESVRRESTSFFAALAEVVEQSGERSRDYSTRIRVTPAVRHGSVW
                     ESVERRWDAMNIALATTVSNIAILQTLFPDMAASAARQDVDLDQLGLRVEDIENRLGV
                     HRYELTRIGMDVNDAIHNPGADQVYWLEMRQGERRDTVLHSAPLQLGEYLRRHLYSRL
                     DSLVLTSATMTTGGDSRFIRSRLGLEDADEITLTSPFDYRSNAMILIPTDLPEPNQPG
                     FDRSAHEAIYSTAVAAGGRTLVLFTSIQAMNQARAALGDRFRDAGLQLVTQHEDGSAE
                     QLAERLRSFDRTVVFGAGAFWEGVDVPGPALSALVIAKLPFPVPTDPIHAARSETFEN
                     GWSDYTMPQTILKLRQGFGRLIRTQTDRGVCVILDRRLISKRYGSQIVNALPSATREY
                     GTISQIGPTVARFLSDGDAS"
BASE COUNT         1999 a         3554 c         3585 g         2077 t
ORIGIN      
        1 cgcccttgtt cgcgagaccc tcgcatcgat cggcacggat gcactgatcc tgaccaaccc
       61 ggcgaatcgc cgctgggcca cgggattcac atcggcgccg ggagtcgcgt tttcccccga
      121 cgttgcggtg atcaccccga gcactgtgga actgctggtc gccgcgatcc acgccgcttg
      181 ggcccggggc gaagcggtgg tcgcgaccaa tgtcatggcg cacaaggggc aattcgcgcc
      241 cagcctcatt gcgtttctga acgagcgcgg atatggatcg gtggcaatcg aggccgatgc
      301 actcccctac cccaacgctc gtgaaatcga agctggtgtc acgaacaccg tcatcagtta
      361 cctgacggga ttgcgggaca cgtggcgcgc atccaaggat ccggccgaga tcgcctcgct
      421 ggagcgcgcg gcccttgtca ccgatcaggt gttcactgac gttgcatcgg ccctgacacc
      481 gggcgagacc gaatccagtg tggctcggcg aatctcggct cgtctcatcg agaacggtga
      541 tggactcggg ttcgacgtaa tcgtcgcgag cggtccgaac gcggcgcgac cgcatcaccg
      601 cccaggcgag cgcgagatcc gggaaggcga gccgatcatc atcgacatgg gcgcgcgcat
      661 cgacgggtat tgcggcgatt tgacccgcac tctttggctc ggggagcctg acgagcgctt
      721 ccgggagctg tacgcggcag tcctcgatgc ccagcgcgcg acgaagtcag tcatgactgc
      781 cggcactcct gtcagggagg tggccgaggc ggccgatcgc gcgctgcagc aggctggctt
      841 cggagagtac attttgcaca gcgtcggcca cggcgtcggg ttggaaatcc acgaatcgcc
      901 ggccatccgc cgggatctcg ataccgtctt gccgctccat gcggtcgtga ccgtcgagcc
      961 cggcctttat gtgcccgaat ggggcgggat tcgagtcgag gatgtcgtag tcgtcggcga
     1021 gtccgagtgc cggaccctga cgacggcccc gaagctcgaa ttcgattgaa cgacatctgc
     1081 atcagaggag cagcacagtg attgatacag gagatctgcg caagggcatg aaccttgact
     1141 atgagggccg gctggtcaag gtcatcgatt tcgcgcacaa caagcagggc cgcggctcgg
     1201 cgcaggttcg gttgacgctg cgcgatatca gaaccgggac gacgactcag cattcggttc
     1261 aggccggaac caagtttcag ccggctcgcc tcgagcgcca gcacgttcaa tacctctacg
     1321 gcgatggcga gcagtttcac ttcatggaca ccgagagttt cgagcagttc gcgctcaacg
     1381 agtcggccgt ctccgatgcg atgaagtacc tcaaggagaa tgacgtcgtc gatctcttga
     1441 cctataccgg ggagccgatc gacctcgagc tgccgatctc ggtcacgctg gaggtcgcgg
     1501 agaccgatcc tggtctcaaa ggcgataccg cgagcggcgg caccaaaccc gcaaccatgg
     1561 aaacgggcgt tgtcgtcagc gtcccgctct tcatcaacat cggcgaccgg ctgcgcatcg
     1621 acacgcgcac cggcgagtac atcgaacggg ccagctagag gagcatggac ttgagcgaga
     1681 ccgaccagga gcacgacgag cacatgtcga ctgatctaac cgaccttgcg gcctttgtcg
     1741 agcgaatggt cacggttgcc cagcgtgccg gcatggcgac cctgaccatc cggaccgagg
     1801 gcctcaagct cgaactgcgg tccggtaccg ccgtcgagat cgtgtcgaat cacgtgccgc
     1861 agccggccgc cctatttgaa gcgccgacgc cgctcatccc ccaggccgag cctgccggat
     1921 acgtgatcac cgctccgatg gtcggaacgt actaccgatc cacttcgccc accgatccgc
     1981 cgctcgtcca acccggggac cgcgtcgagg tcggacaact aatcggcatc atcgaggcga
     2041 tgaagatcat gaacgagatc acggccgatc gcagtggcac ggtgctcgac gtgattgcgg
     2101 agaacgcgac cgcggtcgag tttggctctc ccctgatccg cctcggtcca tagcgagatg
     2161 caatcccagg tcgtcacggt cagccaggtc tcggattacc tccgactgtt gaccgagagc
     2221 gatgcatttc ttagcaacct gctcgtcgaa ggtgaagtta ctgaggtgta cctttccaag
     2281 gcgggtcacc tctatttcac cttgagtgac gggcaggcct cgctcaaggc ggtcgccttc
     2341 cgaaatgcag cactccgggt gcaacacctg gttacgaagg gcgcccatct gaccgtcggc
     2401 ggcagggtga cggtctacag caaagaccag acctgtcaga tttatgtcga attcgtcgac
     2461 catgccggtg tggggggtca ggcgttggcc ctggctcggt tgcgcgctca gctccaggcc
     2521 gatgggctct tcgaacagga gcgcaagaga ccgatcccgg tctatccgcg caccatcggc
     2581 gtcgtcacat caccgaccgg cgccgtcatc catgacatcc agactgttct ccggcggcga
     2641 tttcctttcg tgcacctgat cgtctctcct gccctcgtgc agggtcccgg cgcagttgac
     2701 agtcttctcg cggcgctcga cctcctgatt cacgacggcc gctcggaggt gatcatcatc
     2761 gcgcgcggtg gtggctcgac cgaggacttg agcgcgttca atgacgagcg tctggcccgc
     2821 gcgatctatg cggcgccacg gccgatcatc agcgcaatcg gccacgagac ggacgtctcg
     2881 atcctcgatg aagtcgccga cctgcgcgcg cccacgccga ctgcggccgc cgagctggca
     2941 actcccgaca ttgccgactt tgcgctcgat ttgatcgatg cgcgcgagcg aatggctcgc
     3001 tcccttacgc aggtgctcaa tcaccagcga tcggatctcc agcagctcac acagcggctc
     3061 gaccggtcga gcccgtcgct catttttgcc gagcggcgta ctcgtctggc ggagctcgaa
     3121 tcgcgccttg ggttggccgt ccaccgtcac ctccgcgatg cccaggcaga ctgccggttg
     3181 atttcggttc aactccatgg tgcaggcgtg cgggtgctgg atcggcagcg aattgacctc
     3241 gcgagtgtcc aaagccacat gcgggagctg cctcgggtcc agttcgcgga aaggcgcgcg
     3301 gacctcgacc aatcgcggcg aagagtcgcg acaatcaccc aatcggtcct cgcctccgcg
     3361 tatgccgacc tggacgtgca gaagtcggca ctggtccagc tcaaccctgc cgcggtcctg
     3421 aaccggggtt ttgcgctgct gacaagcgcc gacgggaacg tcattgggag cgtggacgcc
     3481 gtgcgtccgg gcgatcccat ccgcgccgtc gtgcgggatg gcacaatagc ggggaacgtg
     3541 accggcatta ctcgacgcca acgctaacag ggaaccgatg accgaatcag ccacgaccgc
     3601 gcaactgctc gaccgctgca atcagcttgc ggcttctgac gactacgaag ccgtccgcaa
     3661 cggcctgacg caggtcatca ccctgctcga acagccgggg ctcgggctga acgactccgt
     3721 ccgcgcatac gaagtcggac gcaagcttgc cgagcaatgc cagacgctgc tcgatgcggc
     3781 cgaattgcgg attacccagc tcgacggcgc cggcagcgat actggcacgg gaccactcac
     3841 cagccgggtt cgactggagt cgtgaggtga cgaaactggg tgatattctg ccgcccggaa
     3901 aattacctgg cgatcttctg ggccgactgc tcgagcgcca ttcggtgcac gacgagcgcg
     3961 tgctgatcgg tcccggcacg gggcgcgatg ccgcggcgat ccagttcggt gacatggcac
     4021 tggtcgtcaa gaccgaccca atcaccttcc cgaccgaacg ctcggcgctg catctggtcc
     4081 acgtcaatgc caacgacatc gcctgtcagg gtgccgtgcc caggtggctg ctggtcaccg
     4141 cactgctccc gcacgggtcg acctcagccg agtctgcttc gtcaatgttt gcggacgtaa
     4201 atgccgcggc ccggcagatc ggagtttcag tgatcggcgg acacaccgag atcacgatcg
     4261 gccttgatcg cccaatcctg gttggcacga tgctcggcga agtcgcggtc gatcgactga
     4321 tcgatccacg cgacgcctcg ccaggtgacc ggctcctgat gaccaagacc gccgggatcg
     4381 agggaaccgt catcctggcc gacgagctgc gatcacttct catttcacgc ggtgttgcgg
     4441 acaatacgat cgattcggcc gcgacgatga tcgacaaccc gggaatctcg gtcctcgctg
     4501 aggcacgggc cctgtgtatg gccggagcac tcaatgcgct gcatgatccg accgaaggcg
     4561 gaatcgcaac cgccgtgcgg gagctggcgc tggcttccgg ttgcggcgtg atgatcagtc
     4621 gcgacgcgat tccagtcgct gctgaaactg ccgagattgc cactgcgctc aatatcgatc
     4681 cgctcgggat gctggcgtct ggcagcctgc tggcggcagt cccgaaggca tctctcgagg
     4741 tggcagagaa cgcactggct accgcccgaa ttccatttgc gtggatcggt aaactgacgc
     4801 cgcccgaatc cgggatgcgg atccgcacgg gtatggtgga gtccgcgctg cccgagttcg
     4861 aggttgacga agtggcacga gttctagcgg ggtaacagga atgggatggg ttttgaccgg
     4921 cagctcgaat gccgacgtcg gcttcgagaa tggttcgcgc gtgctccgga atggcggatc
     4981 cgcgattgag gcggtcatca ccacgattcg cgaggtcgaa gcgaacccgg acgatcatag
     5041 tgtcggctat tccggcttgc cgaacctgct gggcgacgtg gagctcgacg caagcatcat
     5101 ggacggccgc ggcctccgcg cagggtcggt cggcgcgctc aagaatcacc aggacgcgat
     5161 cgatcttgcc cgctgtgtga tggaagagct gccgcacgtc ctgatcgtcg gcgatggcgc
     5221 cgagcgattt gccggcgagc agcagctccc gaaagtcgat ctgctgactg atgaggcaaa
     5281 ggccatctgg ctccagcgcg cggatcccac attgcagcgc tcaaagtact acgagcggat
     5341 gtcggaactt gtccgcgaga ccacgagcga tccccagttt ccggagccgc cgcacggaac
     5401 cgtcaatgtc atcgccaggg acctgcatgg caacatcgcc tgcggggtca gtacctccgg
     5461 ctgggcctgg aagtatccgg gccgactggg cgactcgccg atcatcggcg ccggcattta
     5521 cgccgacaat cgcttcggcg cggcggcatg caccggtcgc ggagagatgg cccagcgctg
     5581 cctgacggcc cacagcgtgg tcacgttcat gcgattcggc atgagcctgg aagacgcgct
     5641 gcggatggcc gctgaggacc ttcgctcgct cgacgatccc tacgcgagcg aaatgaacat
     5701 cgtcgcgatc gatgtcgatg gaacgcataa cgcggtatcg accagtgaag gcaagaccta
     5761 catcttccag acagatgcca tgccctcgcc cgagtacggg gaacgcatcc acgtcccgct
     5821 ctgctgattg gagaaaagaa acgggccggc tggattaccc agccggcccg ttttcagttt
     5881 ctgacgtccg acgtccgaag tctgtggact ctctacacgt ccttgttcag ggtcgcgagg
     5941 aactcggcgt tgctgccggt cttcgagagt cgaccgagaa cgagctcggt gccttccgat
     6001 ccacccagca tggaaaccat gcgacgcatc gtccagacct ggcgaagagt ggcgtcgtcc
     6061 atgagcagct cctcgcggcg ggtgccggat cgctggatgt cgatggccgg gtagatccga
     6121 cgctcggcca gtttgcgatc gaggtgaagc tccatgttac cggtgccctt gaactcctca
     6181 taaatgacgt catccatgcg gctgccggta tcgaccagac aggtcgcgac gatcgtcagg
     6241 gagccgccgc cttcgatgtt gcgggcggcg ccgaagaagc gcttgggcgg atagagggcg
     6301 accgggtcga taccaccgga gagcgtgcgt ccgctcggcg gcacggccag gttataggcg
     6361 cgggcaagac gtgtgatcga gtcgagcagg atcgtgacat cccggccggc ctcgaccagg
     6421 cgcttggctc tctcgagtgc catctcggcg accttggtat ggtcctcgac aggctcgtcg
     6481 aaggtggagg aaatgacctc gccctcgaca gagcgcttca tgtcggtgac ctcttccggt
     6541 cgctcgccga tcagacagac catcaggtgc atgtcgggat cgttcgaggt gatgccgttg
     6601 gcgatcgact tgagcaggag cgtcttgccg gctttcggcg gtgacacgat cagaccacgc
     6661 tggcccttgc caaccggcgc gaccaggttc aggagtcggg tcgagagaat gttcggcgtg
     6721 gtctcgagat tgatcatctc gagcgggaag attggggtca gcgagtcgaa attcggccgc
     6781 cgccgggcta cttccggatc gacgccgttg acggcctcga cgcgaagcaa actgaagaac
     6841 ttctcgttgt ccttcggcgg gcgaacctgg ccggaaatgc gatcgccggt gcggagaccg
     6901 aagcggcgaa tctgcgactg cgagacgtag atgtcgtccg agcccgggag caggcgtgag
     6961 ccccggagga agccataccc ctcgtcgacg atctcgagga ccccgtcgcc gaagatattg
     7021 ccctccatct cggtccggga ccggaggagc cggctgatca ggtcgggccg cttcatgcgg
     7081 ctgaatccct gaatctcgaa tgtgcgggcg agctcgatca acgcctcggg ctccatctgg
     7141 tcgagctcag tgacggtgaa gtggggcaca tcggggttcg tcccgttgga cgtgaactcc
     7201 tgcggctcca tttcgtggga tcggatctcg cgcggaccgt aatcggtccg ctcgccccgc
     7261 tcggcgcgat tgccgcgttc agggcgatca ccacggtcgt gccgatcgcg gcgcccaccg
     7321 cgatcacgat cgcggtcatt gcggccaccg ttgcgatggc ggcggctgaa ttcgcgctcg
     7381 cgaggttcgg accgctcggc agaggtatcg gtcatgtccg gagcgtcgat gacggcggtc
     7441 gcggtctgac cctcgtctgc catggcggta ccgtcctggc tctcaggtgc ggttcggcgc
     7501 cgaccccgcg tagtgcgcgg cttgggttcc tggacctcgc tcgggccatt ttcggccggc
     7561 gtgggattca ggatttcctg gtcagacaat tgagttcctc ctcatcaggt agggtcggtt
     7621 cgtggataca cggtttggtt ggcacatctt tggtttccgg ttgagatccg atatgtgggg
     7681 acgatgatca gccctggcgg ttcgggtgcg aaatcaggct ggcgggcgtc agcgtttgtt
     7741 ccttgactca tggttgcgcg tcggtctcgc ttatctcaag agacacacgg aattggggcg
     7801 caacgagctt aaatcggaat acgaatgcct gcttggcaag gggttggatc gccgattggc
     7861 tagtacatag gataccaccc ggtcaaaacg agtgtcaaca agagtcaagg cgcgaatgcc
     7921 gcgatttact gtgattttcg tcctaatcga atggtagccc gctcagctgg cgcagcttgt
     7981 ccagacggtg cgtggcgtcg atccggcgga tggttccggt gcgcgatcgc atgaccagtg
     8041 acgacgtccg ggcgccattc tcgtagaact gcacgcctcg taccagctcg ccgtcagtga
     8101 tgccggtggc cgcaaagaac acgttgtccg acttgaccag atcgttcgtg ctgagcacct
     8161 catcgagcga gatgttctgc tcgatcgcga tcttccgctc gtcgtcattg cgtggatacc
     8221 gaacgcattg gatctcccca ccaagggact tgatcgcaca ggccgcgacg accgcctcgg
     8281 gcgcgccgcc gattccgagc aagacatcga cgccggtgcc cggagtggcc gccatgaccg
     8341 ctccggccac atcgccatcc gggatcatct tgatccgggc accggtctcc cggatctcgc
     8401 ggatcagctc atcgtggcgg ggtcggtcga gcacgacgac ggtcagatcg ccgatggtca
     8461 ctccttttgc cgccgcaacc cggcgcaggt tctcctgcac cgaggccttt acgtcgacga
     8521 aaccggcggc ttcaacgccc acggcgatct tgttcatgta gctgatgtcc ggggaggcca
     8581 acatggtgcc tcgctcggcg accgcaacga cggcgatcgc gttcggcatg cccagcgaga
     8641 gcagtcgcgt accgtcgatc ggatccaccg cgatatcgac gtgcggcgcc gccccggtgc
     8701 caacctcctc gcccacgtag agcatgggag cctcgtcttt tgcgccctcc ccgatgacga
     8761 cgatgccgtc catatcgagc gaattgagga cgttccgcat cgcgtcgacc gccgcctggt
     8821 cggccgcgtt cttgtcgccc cggcccatcc atcgcgcggc catgatcgcg gcggcctcgg
     8881 tgacgcgggc aagctcaagg gcgaggttgc gatcgacggt gctggcggtg ctcatgacgc
     8941 gtctccgtca ctcaggaatc gtgcgactgt cggaccgatt tgcgagattg tgccgtattc
     9001 gcgcgtggca ctgggaagtg catttacgat ctgcgagccg taccgcttgg agatcaatct
     9061 gcgatccagt atcacgcaaa ctccgcggtc tgtctgcgtc cgaatcagcc ggccgaatcc
     9121 ctggcgaagc ttcaggatcg tctggggcat ggtgtagtcc gaccagccgt tttcgaacgt
     9181 ctccgatcgt gccgcgtgga tcggatcggt cggaaccggg aagggcagct tggcgatcac
     9241 gagcgcgctc aacgccggtc cgggcacatc gacaccttcc cagaatgccc ccgcgccgaa
     9301 aacgacggtc cgatcgaacg aacgcagtcg ttcggcaagc tgttcggctg acccatcctc
     9361 atgttgcgtg acgagctgca gccccgcgtc gcggaatcga tcaccaagtg cggcccgggc
     9421 ctggttcatc gcctggatcg aggtgaagag cacgagtgtg cgcccgccgg ccgcgacggc
     9481 cgtactgtaa atcgcctcgt gcgcggaccg atcgaacccg ggttggttcg gctccgggag
     9541 atcggtcggg atcaggatca tggcattgga gcggtaatcg aacggcgacg tgagcgtgat
     9601 ctcgtccgca tcctccaggc ccaaacggct cctgatgaag cgggaatcgc cgccggtcgt
     9661 catcgtggca ctggtcagga cgagcgagtc gaggcgtgaa tagaggtgcc ggcgcaggta
     9721 ttcgcccagc tgcagcggcg ccgagtgaag gacggtgtct cggcgctcgc cctggcgcat
     9781 ctcaagccaa tagacctgat cggcgcccgg attgtggatc gcgtcgttga catccatacc
     9841 gatccgggtc agctcgtacc ggtgcacacc caggcgattc tcgatgtcct cgacccgcag
     9901 accgagctga tcgaggtcga cgtcctggcg cgccgccgac gccgccatgt ccgggaaaag
     9961 tgtctgcagg atcgcgatgt tcgagacggt cgtcgccaat gcgatgttca tcgcgtccca
    10021 gcgccgttcg accgattccc agacggaacc gtgccgcaca gccggcgtga cccggatccg
    10081 cgtcgagtaa tcgcgggagc gctcgcccga ctgctcgacc acctctgcaa gcgcggcgaa
    10141 aaacgaggtt gactcccgcc ggaccgattc aatcgcggtc tcgacggcct cgatcgactt
    10201 aagcacccgc tccgtctggt cgtgggtcgg cgtcaacaat ttgcggttat ggaccagggc
    10261 gccgatctcg accaggaggc ccggctgtcc acgccgattg tcgccggcca gggcattcag
    10321 ggtctggctg atcaggcgac tcgaaatcga gacttggaag ctggtggtcg cctggtcctc
    10381 gaggtgatgc gcctcgtcga tgatcaggtg cttcgcttcg ggcagggcct cgccgtcctg
    10441 cacgacatcg gtcaggagca gcgcgtgatt ggtgatgacg acatgtgccg catccgcgga
    10501 tcgccgggcc cgatagagga agcattggtt ccgctgctgg aactggcagc ggctggcatc
    10561 gcacgcttcg ccatcggctg atacgcgatt gaacactgcc gcctgtccgc gctcgagcgg
    10621 caaatctgcc tgttcgccgg attcggtcaa gttgagccag atattcgccc gggcccggaa
    10681 tgacgcttct tcgtcattgg ccggcaccgg cttctgctgc atttccgcaa accagcggcg
    10741 caggcagaga tagttgctgc gccccttcag gacctgcaca cgcggctcgc agccgggatg
    10801 cgactcccgc acggccgcga ccgccaatgg tgcgtccttg ctcgcaagtt ggtcctggag
    10861 aacccgggta ttcgtcgcga caacgacctg ctctccggtc atgacggcat tcgtcagcgc
    10921 gggcacgaga taggcgagcg acttgccggt gcccgtaccc gcttccgcga tcaactcgcc
    10981 gccgtcgcgc agctcaccgg ccaccgcaac ggccatctcg cgttgctgcc gccgcggctc
    11041 ccaggcatcg aaacgtttcg agatcggccc gccgatgtcg aaggaggccg cgatttcgtc
    11101 cggctcgacc ctgtcggagt tcttgacccg cttcaaccgc tcgggctttt cacggttcgt
    11161 caggaaggcc agttctgggg gccgcgccga tgccgcgccg tgcatggcct ggctg
//
LOCUS       DATAOZ010000039         6221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1336401, whole genome shotgun sequence.
ACCESSION   DATAOZ010000039 DATAOZ010000000
VERSION     DATAOZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336401"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VHR64_01115"
     CDS             <1..446
                     /locus_tag="VHR64_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX3301455.1"
                     /translation="GCVEAMASGPAIIGEANRRVVQGFTTRLTEMIGDDLNRMTPGLV
                     EQAAAEGDPAAREVLERAGYFLGLSMAGMIAALAPDVVVIGGGVVKPHGVYWTAFEST
                     ARASNTVTDMSRVSFVPASLGYEAGVIGAALWGRMVERGEAKPAA"
     gene            complement(600..1151)
                     /locus_tag="VHR64_01120"
     CDS             complement(600..1151)
                     /locus_tag="VHR64_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301456.1"
                     /translation="MSDRETLASELRQMIEHIRAYVPDAVVNTRLAHSDQNGVVIHAQ
                     IMGGSGPSGSAHASAAVGDASPELAENRAIARAMIAMGLPAIQESVGPKLHVAQPEQP
                     SRLVQFPAQEPERADTKPTEPESESDSDPKPEDISWTAFWTWAKANGFPDKKTLEEAI
                     GQPIDRLQPGQIRKLAQTVIDAR"
     gene            1307..1570
                     /locus_tag="VHR64_01125"
     CDS             1307..1570
                     /locus_tag="VHR64_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301457.1"
                     /translation="MNRARIDLLDMIWPETGLQVTTTAPVKERDTLTENDQIELSLDF
                     VTLSLTPTEFIQLAASLRLSVDGLLDHHPGLQRAVVAAFDIKM"
     gene            complement(1560..2585)
                     /locus_tag="VHR64_01130"
     CDS             complement(1560..2585)
                     /locus_tag="VHR64_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3301458.1"
                     /translation="MVKIGVLGAGFMGSTHARALAAIDDVEIVGIYGQSEKRAKPLAE
                     ELGTTWTTDLEKLIDDPSVDGIDICLPGPQHREVTELVIKAGKHVLLEKPITLLIEDA
                     DALVDLAQSTDRIFMVAHVLRFWPEYVELQKLATSGTYGRPIQGLAYRRQPFPAWSSL
                     FGQSELTGGAVVDMMIHDYDALNWVFGEPKSVVARGTRNPKSGGYDQVQVMIDYGDAW
                     AQVDGGMMMPESYPFTSSLQVLCETGAVEYNFQAGGRSVEMGAGRNELKLFPNEGDPC
                     VVTAEQADPYAAEVAYWVDCIRTGQAATRATPADARTALKVALAARRSLDNDGLVAEP
                     VSAAPTS"
     gene            complement(2662..3120)
                     /gene="tnpA"
                     /locus_tag="VHR64_01135"
     CDS             complement(2662..3120)
                     /gene="tnpA"
                     /locus_tag="VHR64_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX3301459.1"
                     /translation="MEGQGVSFWRCHYHLIWTTWQRHPAIDAVREGVIARCVASIANE
                     QKLKLHAVGIVEDHIHVAISIPPRLAVASVVQQIKGSSSNQFGKANRSEIDGWPGWQS
                     EYGVLTFGEASFDRIVSYVANQKEHHRNQTLWAPLEQFRADQPGIGAVSP"
     gene            complement(3209..4024)
                     /locus_tag="VHR64_01140"
     CDS             complement(3209..4024)
                     /locus_tag="VHR64_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3301460.1"
                     /translation="MTDRLAASTNSYHTYSLEEALDGIAKAGFKSVELTSVPGWTEHV
                     RRDATEEELAYVKELLGKHGLAAVSLSGHSDLVSESGIAEFRKALKIAKKLGIRYVTT
                     STGGHDASSAGSLDEQREKFMANIRPLADEAKAMGIDICLETHGGLLATGEISKKLIQ
                     DIGKENISINYDPGNVIFYGGVRPEDDIKKSADTVGHMHVKDQIGGPGVWNFPPTGTG
                     EVDFKTIFAELDKAGFNGICSVEVEFQGEPWPALADVDAALKTSYDFARQYVK"
     gene            complement(4052..4441)
                     /locus_tag="VHR64_01145"
     CDS             complement(4052..4441)
                     /locus_tag="VHR64_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301461.1"
                     /translation="MPDNSRGTRTFHHVGLRATEPQPNENFVTATRVWVTDPNDSPYR
                     IEFLRYEPDSYLDERFKNTPHVAWVVDDIDPWIEGKEIAIAPFEVGDPAFVRVAFVWE
                     EGMISEYMAFKPGAVWFDPNDVGKEQA"
     gene            complement(4550..5707)
                     /locus_tag="VHR64_01150"
     CDS             complement(4550..5707)
                     /locus_tag="VHR64_01150"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3301462.1"
                     /translation="MGRLDEVITDLTSAQSNRRTVMKQAAALGLSFTALHRVKLSVLA
                     GEAGKVRWVSPRGTLDVLDDYPYWVAQKMGYFGSIQTEIEPGPTSGQGKLVDADQSDM
                     SYSSPGVYSFDLEAGVPLVSVFQMGAYDVFDFAFPKGAGITDLKQLEGKKVLIGDASW
                     QGICDPEFAQMGADPKKIEYLVAGLSWQENLKAGQADAALSWEGLRAQWKSLGYDFDY
                     LLGMDFSKFPANSFQIRQKDFEDTSLADTYTQYLRGWAMGLEFGHNNPRAATQITMEA
                     RPKVKEAFPDLGVAVESMQQLATVFRGDFANRQGWGWHDESAWQLFFDTIKSIGQITK
                     DIKAEDVIKNDYVAGANDFDHDKVKADADGFQLSAEFSALPAPSPEASPAS"
     gene            complement(5855..>6221)
                     /locus_tag="VHR64_01155"
     CDS             complement(5855..>6221)
                     /locus_tag="VHR64_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3301463.1"
                     /translation="AAGPRVMINAATGFRRTDLAQIALARSYGASTFQIFRKVRFPLA
                     LPMIIVGLMVGAIFGVLTAVGAEMVSGKDGLGNQIIYWQTFSKYNYVWSIITILALIG
                     IGIYVIFYQIGKRWASWQA"
BASE COUNT         1034 a         1853 c         2103 g         1231 t
ORIGIN      
        1 atggctgcgt cgaggcgatg gcaagcggtc cggcgatcat cggcgaggcg aatcgccgcg
       61 tagtccaggg cttcacgacg cgattgaccg agatgatcgg cgacgacctg aaccggatga
      121 cgccggggtt ggtcgagcag gcggcggcgg agggcgatcc tgccgcgcgc gaggtgttgg
      181 agcgcgccgg atacttcctc gggctgagca tggccgggat gatcgcggcg ctggcgccgg
      241 acgtggtcgt gatcggcggc ggagtggtca agccgcacgg cgtgtactgg accgccttcg
      301 agtcgaccgc gcgggccagc aacacggtca ccgacatgtc gcgcgtctcg ttcgtgccgg
      361 cgtcgctggg atacgaggcg ggggtgatcg gagcggcgct ctggggtcga atggtcgagc
      421 gcggcgaagc caagcctgcg gcttgacgat cattcgatcc cagcgcggtc cacttcgggg
      481 tcgcccggat gggcaatgtg gtcggtcggc tgcaactggg gcgggtgccc tgaatcccag
      541 gccggccaca ggggaccggt gctacgtcat tggcgcatga gcttcgatgc gcccgaaggc
      601 taccgggcgt cgatgacggt ttgagcgagc ttgcggattt ggcccggctg gagccggtcg
      661 atcggttggc cgatcgcttc ctccagggtc ttcttgtccg ggaagccgtt ggccttggcc
      721 caggtccaga aagcggtcca ggagatgtcc tccggtttcg gatcggagtc ggactcggat
      781 tcgggctcgg tcggcttcgt gtcggcgcgc tccggctcct gggccgggaa ttgaacgagg
      841 cggcttggtt gctcaggttg ggcgacgtgc agttttggcc caactgattc ctggatcgcg
      901 ggcaggccca tggcgatcat ggcgcgggcg atggcgcggt tctcggccag ttcaggcgag
      961 gcgtcgccga ccgccgcgct ggcgtgtgcg gagccggatg ggccggagcc gcccatgatc
     1021 tgggcgtgga taacgacgcc gttctggtcg ctgtgggcga ggcgcgtgtt gacgaccgcg
     1081 tcgggcacat aggcgcggat atgctcgatc atctgccgca gctcgctggc cagcgtttcc
     1141 cggtcactca cgacgttccc tgaaccccca ttcccgatct tgtcgatgcc ctgagtatac
     1201 agagtcagta catcagttcg acagggagcg agatatccgc gttcgcccat ccgtgactca
     1261 ttcgcggcac aatgggggtg ttggactgcg tagcagggag atgtctgtga atcgggcacg
     1321 gattgatttg ctggacatga tctggccgga aacgggattg caggtgacga cgacggcgcc
     1381 ggtcaaggag cgggatacgt tgaccgagaa cgatcagatc gagctcagcc tggactttgt
     1441 gacactgtcg ctgacgccga ccgagttcat ccagctcgcg gccagcctgc ggttgagcgt
     1501 cgacggcctg ctcgaccacc acccggggtt gcagcgggcg gtggtcgcgg cgttcgatat
     1561 caagatgtag gcgcggctga gaccggctcg gcaaccagtc cgtcgttgtc gagcgagcgg
     1621 cgggcggcga gggcaacctt gagcgcggtc cgggcatcgg ccggcgtggc gcgggttgcg
     1681 gcctggccgg tccggatgca gtccacccag taggcgacct cggcggcgta gggatcggcc
     1741 tgctcggccg tcacgacgca cgggtcgccc tcgttcggga agagcttcag ctcgttgcgg
     1801 ccggcgccca tctcgacgct gcggccaccg gcctggaagt tgtattcgac ggcgccggtc
     1861 tcgcagagga cctggagcga cgaggtgaac gggtagcttt cgggcatcat catgccgccg
     1921 tcgacctgcg cccaggcatc tccgtaatcg atcatcacct gaacctggtc gtagccgccc
     1981 gatttcgggt tgcgagtgcc gcgggcgacg accgattttg gttcgccgaa gacccaattc
     2041 agggcgtcgt agtcgtggat catcatgtcg acgacggcgc cgccggtcag ctccgactgg
     2101 ccgaagaggg acgaccaggc agggaatggc tggcggcgat acgcgaggcc ctggatcggc
     2161 cggccatagg tgccgctcgt ggccagcttc tggagctcga cgtattccgg ccaaaagcgg
     2221 agcacgtgcg cgaccatgaa gatgcggtcg gtgctttggg cgaggtcgac cagggcgtcg
     2281 gcgtcctcga tcaggagcgt gatcggcttc tcgagcagga catgcttgcc ggccttgatc
     2341 accaactcgg tcacttcccg gtgctgtgga ccgggcaggc agatatcgat gccgtcgacc
     2401 gacgggtcgt caatcagttt ttcaaggtcg gtcgtccagg tagtgccgag ctcttcggca
     2461 agtggtttgg cgcgcttttc tgattggccg tagatgccga caatctcgac gtcatcgatg
     2521 gcggcgaggg cgcgggcatg ggtactgccc atgaagccgg cgccgaggac tccgattttg
     2581 accatgcctt gtctcccccg ttcttccgtg caacgacgtt acccggggtt gaaaccccgg
     2641 gcttagttca cgaagtccct ttcagggact gactgcgcca attccgggtt ggtcggctcg
     2701 gaactgttcg agtggtgccc atagcgtttg attgcggtgg tgctccttct ggtttgcgac
     2761 gtaggacact atgcgatcga acgaggcttc accgaatgtc aggacgccgt attcagattg
     2821 ccatccgggc caaccatcga tttcggaccg attagctttg ccgaattgat tgctgctcga
     2881 gcccttgatc tgctgtacga cggatgcgac cgctagccgg ggcggtatcg agatagccac
     2941 gtgtatgtga tcctcgacta ttccgaccgc atgcagcttg agcttttgtt cgttggcaat
     3001 cgacgcgaca caccgcgcaa tcacgccttc gcgtacggca tcaatcgccg ggtgtcgttg
     3061 ccacgttgtc cagatcaggt ggtaatgaca ccgccaaaac gacacgccct gcccttccac
     3121 caatccctag tccctgaaca gggacttcgt gtactcagac cggggtttca accccgggac
     3181 cccgaagccc ggatgcctcg cccccgtcct acttcacgta ctggcgcgcg aagtcgtagc
     3241 tggtcttcag ggcggcgtcg acgtcggcga gggcgggcca gggttcgccc tgaaactcga
     3301 cttcgacgga gcagatgccg ttgaagccgg ctttgtcgag ctcggcgaag atggtcttga
     3361 agtcgacttc gccggtgccg gtgggcggga agttccagac gccggggccg ccgatctggt
     3421 ccttgacgtg catgtggccg acggtatcgg ccgacttctt gatgtcgtcc tcaggccgga
     3481 cgccgccgta gaagatgacg ttgccggggt cgtagttgat gctgatgttt tccttgccga
     3541 tgtcctggat cagcttcttc gagatctcgc cggtcgcgag gaggccaccg tgggtctcga
     3601 gacagatgtc gatgcccatc gccttggcct cgtcggccag cgggcggatg ttggccatga
     3661 acttctcgcg ctgctcgtcc agcgacccgg ctgaggaggc gtcgtggccg ccggtgctgg
     3721 tggtcacgta tctgatcccg agctttttgg cgatcttgag cgccttgcgg aactcggcga
     3781 tgcccgactc gctaaccaga tcggaatggc cggagaggct gaccgcggcg aggccatgct
     3841 tgccgagcag ctccttgacg taggcgagtt cctcttcggt ggcatcgcgg cggacgtgct
     3901 cggtccagcc ggggacggag gtcagctcga cgcttttgaa gccggctttg gcgatgccgt
     3961 cgagcgcttc ttcgagcgaa taggtgtgat agctgttggt cgatgccgca aggcgatcgg
     4021 tcatgcgagt tccccttccg atggtgttgc gttacgcctg ctctttgccg acgtcgttcg
     4081 gatcaaacca gaccgcgccg ggtttgaacg ccatgtattc ggagatcatg ccctcctccc
     4141 agacgaaggc gacccgaacg aaggcagggt cgccgacttc aaagggcgcg atcgcgatct
     4201 ccttgccctc gatccagggg tcaatatcgt cgacgaccca ggcgacgtgc ggcgtgttct
     4261 tgaaccgctc gtcgaggtag ctgtcaggct catagcgaag aaattcgatg cgataggggc
     4321 tgtcgttcgg gtcggtcacc cagacccggg tggcggtgac aaagttctcg ttcggttgcg
     4381 gttctgttgc ccgaagcccg acgtggtgaa acgtgcgggt tccccggctg ttgtccggca
     4441 ttcatgtcct caatcgatgc ggcgcgtgcc gcgtatggaa ccgggcgatc accccgggat
     4501 ggggagatcg cccggtcaac ggttaccgat tcgatcccgt gcgcaagcgc taggacgccg
     4561 ggctcgcttc cgggctcggg gccgggaggg ccgagaactc ggcggagagc tggaagccat
     4621 cggcatcggc cttgaccttg tcgtggtcga agtcgttggc gccggcgacg tagtcgttct
     4681 tgatcacgtc ttcggccttg atgtccttgg taatctggcc gatcgacttg atggtgtcga
     4741 agaacagctg ccaagcggac tcatcgtgcc agccccaacc ctgccggttg gcgaagtcgc
     4801 cgcggaagac ggtggcaagc tgctgcatcg actcgacggc aacgccgaga tcggggaagg
     4861 cttccttgac ctttggccgg gcctccatgg tgatctgggt ggccgcgcgg gggttgttgt
     4921 gaccgaattc gagacccatc gcccaaccgc gcaggtactg cgtgtaggtg tcagccaggc
     4981 tggtgtcctc gaaatccttc tgccggatct ggaatgagtt ggccgggaac ttcgagaagt
     5041 ccatgccgag caagtagtcg aagtcatagc caagcgactt ccactgcgcg cggaggcctt
     5101 cccatgaaag ggcggcatcg gcctggccgg ccttgaggtt ttcttgccaa ctcagtcccg
     5161 cgaccagata ctcgatcttc ttcggatcgg cacccatctg ggcgaattcg ggatcgcaga
     5221 tgccctgcca tgacgcatcg ccgatcagca ccttcttgcc ttcgagctgc ttgagatcgg
     5281 tgatcccggc gcccttcggg aaggcgaaat cgaagacgtc gtaggcgccc atctggaaga
     5341 ccgaaacgag cggcacgccg gcctcgaggt cgaacgagta gacacccggc gaggagtagg
     5401 acatgtccga ctgatcggca tcgaccagct tgccctggcc agaggtcggg ccgggctcga
     5461 tctcggtctg gatactgccg aagtagccca ttttctgggc aacccaatag ggatagtcgt
     5521 cgagcacgtc aagcgtgccg cgtggtgaga cccagcggac cttgccggcc tctccggcca
     5581 ggaccgagag ctttacccgg tgcagcgcgg tgaacgagag gccgagtgcg gctgcctgct
     5641 tcataacggt acgacggttc gattgcgcgc tggtcagatc ggtgatgact tcatcgagac
     5701 gacccatacg tgtttcctcc gtacgctgtg cgcttcgttg cgccgttacc aactaacggc
     5761 gtattgcccg tggcccgcgc gaccctcacg cacacccgag cagagcgccg cgatcatttc
     5821 tgaccgcgga gaactcccac acctcgaccg gcagctatgc ctgccagctc gcccaccgct
     5881 tcccgatctg atagaagatc acgtagatcc cgatgccgat cagcgccagg atggtgatga
     5941 tcgaccagac gtagttgtat ttggagaagg tttgccagta gatgatctgg ttgcccagac
     6001 cgtctttgcc gctgaccatc tccgcgccga ccgctgtcag caccccgaag atcgccccga
     6061 ccatcagacc gacgatgatc atcggcagcg cgagtgggaa gcggaccttg cggaagatct
     6121 ggaaggtact tgcgccgtag gaacgggcga gcgcgatctg ggccagatcg gtgcggcgga
     6181 agccggtggc ggcgttgatc atgaccctcg gaccggcggc t
//
LOCUS       DATAOZ010000040         5834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8701521, whole genome shotgun sequence.
ACCESSION   DATAOZ010000040 DATAOZ010000000
VERSION     DATAOZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8701521"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VHR64_01160"
     CDS             <1..750
                     /locus_tag="VHR64_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX3301464.1"
                     /translation="NARNAVALLASCRNLALVGFHMHLGSQVSTHEAMPIAIERQVAF
                     AAEVRDRHGIVARRYSPGGGLSIAYTEQGVDGDPAEWAEVITSAYSVAWEKHGLPAGT
                     IIAEPGRWLVGPAGVALYRVGSMKRIAGVRTYVAVDGGMADNIRPSMYGATYTAEIAS
                     RQAGGSVERVRIAGRYCESGDVLIDDIALPEVEVGDLMAIPASGAYQLPMASNYNAVP
                     RPAVVMVTNGAARIVRRRETFEDVFAAEAFE"
     gene            936..2597
                     /gene="rpoD"
                     /locus_tag="VHR64_01165"
     CDS             936..2597
                     /gene="rpoD"
                     /locus_tag="VHR64_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX3301465.1"
                     /translation="MSDTYLPNEDADDDELEPEFLDDALPIDETGGVGTVDELDQENG
                     VLIGNGPSDELNAGSDENADGAIAVDALLDAIPSSDGDPSGIDALSHRLAELNSVLAV
                     SDDEIEEDVDQDVDLASDAAPHLEVETTNYDIGAISLEDPVRMYLREIGRVPLLNAQR
                     EVELASAMERGEYLKAKQHQLTTDFGEAPEADVLGRAIYHSFKQGWPHVQAMYVAVHG
                     DERLPAKSLMLKRVLPMTQLPEEAVSEVCQSLGMTSDELEESMRVRSVEWELLPVSIQ
                     NLIRDTPDWPDDAEIDALFRSQAARLQRRWDEMTRNGDLAKVALTEANLRLVVSVAKK
                     YAGRGMSMLDLVQEGNLGLIRAVEKFQHHKGFKFSTYATWWIRQAITRAIADQARTIR
                     IPVHMVETINKLIRTSRRLQQELGREPTSDEIAVAMEMPPERVREIMKISQEPVSLEM
                     PIGEEEDSNLGDFIEDQKALPPADAASRKMLKEQVDDVLGTLSERERAVLAMRFGLDD
                     GRSRTLEEVGREFGVTRERIRQIEAKALRKLRHPSRAKKLKDFLD"
     gene            complement(2882..3715)
                     /locus_tag="VHR64_01170"
     CDS             complement(2882..3715)
                     /locus_tag="VHR64_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3301466.1"
                     /translation="MQFGFVLPGGSAPEQLELAMLVDQSGWDAVFVWEAAYGVDAWSL
                     LGAMAARTERVKLGTMLTPLPWRRPWKVASQVVTLDQLSNGRAILAVGLGAVDNFLGN
                     TGEERDRKTRAAMMDEGIDLIKGLWRGERVFSGQHYEMDLSGRTDLSDVAQPVQANIP
                     IWVVGAWPSEKSMTRVLRCDGVLPNIIRDGQHGSVEPDDVRAMLAWLRERGGAGEGFD
                     FVVEGETIPGDANEAQEKIGPLRNAGATWWLETRWQLLGDREQQYQEVRNRIEAGPPR
                     F"
     gene            3813..4475
                     /locus_tag="VHR64_01175"
     CDS             3813..4475
                     /locus_tag="VHR64_01175"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX3301467.1"
                     /translation="MSEQSLPLDDTVLFDPAAPGAIEMAAELIAQGGVVAIPTDTVYG
                     VAASLSHPEALRRIYEIKGRDRAKPLPILVSSTDALARLAPEIDPNIALLLNVYWPGP
                     LTIVIESSPGMPAEVLASDDTVAVRLPNHMLAIELIERAGGAVACTSANISGRDPAAS
                     AQDVLATVGPALDGVVEGGIAPGGVPSTIIRIADGEVLTVREGPIPIEHIRATWDSFR
                     SE"
     gene            4539..5477
                     /locus_tag="VHR64_01180"
     CDS             4539..5477
                     /locus_tag="VHR64_01180"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301468.1"
                     /translation="MVSDFGEGVTSQDQTIAGERKDPRHDPATTGGTVLGSVHPPAYG
                     RGLLVSIVAAAIVFALAIFASGGKEEFTGIPMTQTTGSLFWGLSILAIILVGVASQYA
                     EGAAIRVAESLGQEPARRNQLPSAWAVPAASMATALLLVATYHNKVMLIAGPAIAFLG
                     VAGGLFARDLLEDTMEGAERTAALIHTIVIHAIAFLGLSAVYMNKMSGWYGGPIVFLL
                     TAVLMFEHLERAGLPDGLRLLYSSLSGGVMILALLAVNWWPTYGWTGGAALLLCFFVL
                     AGLGSVRGERQSLRDRDLIEYGAVFVGGLLILAITL"
     gene            5494..5673
                     /locus_tag="VHR64_01185"
     CDS             5494..5673
                     /locus_tag="VHR64_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301469.1"
                     /translation="MSNAGSKRLFDLYERMDRLEELIEEMEELGISTREEAEQMIVEL
                     DGQIEELESHDDSGD"
BASE COUNT         1025 a         1820 c         1948 g         1041 t
ORIGIN      
        1 aacgcgcgga acgcggttgc cttgctggcc tcgtgccgga atctcgcgct cgtcgggttt
       61 cacatgcacc tggggtcgca agtcagcacg catgaggcga tgccgattgc tatcgagcgg
      121 caggttgcct tcgcggccga ggtgcgggat cggcatggca ttgtggcccg gcggtactcg
      181 ccaggtggcg gcctgagcat cgcgtacacg gagcagggag tcgatggcga cccggccgag
      241 tgggcagaag tgatcacgtc ggcgtactcc gtagcgtggg aaaagcatgg ccttccagcg
      301 ggcacgatca ttgccgaacc cggccgctgg ttagttggtc ccgcgggagt cgcgctttat
      361 cgcgtgggct ctatgaaacg gattgccggt gttcgcacgt atgttgcggt agacggtggc
      421 atggctgaca atatccgccc ttccatgtac ggcgccacat acaccgccga gatcgctagc
      481 cggcaggccg gcggatcggt tgaacgggtg cgcatcgctg gacgctactg cgagtcaggg
      541 gatgtcttga ttgacgatat cgcattgccc gaggtcgaag tcggcgacct gatggcgatc
      601 ccggcatccg gcgcatacca actgccgatg gcgagcaatt acaacgcggt gcctcgcccg
      661 gcggtagtga tggtgacaaa tggagcggcg cgaattgttc ggcggagaga gacatttgag
      721 gacgttttcg ctgcggaggc cttcgaatag cggttctggt attgcgcaaa gcgaatgttt
      781 tgcgtaccct ttgcgttgtc ccaaccggaa cgcagcgtgc gcggcctggt cgccttgggc
      841 cgtgttggct gatctctccc aggctgtcgt ggctgagcca gcctgatcag gctggatgtt
      901 tttgacctat tcggattctg aggggaaacg ttctgatgag cgacacctac ttgccgaatg
      961 aagatgctga cgacgatgag ctcgagccgg aattcctcga cgacgcgcta ccgatcgacg
     1021 agacaggggg ggtaggcacc gtggacgaac tggatcagga aaacggcgta ttgattggga
     1081 atgggccaag cgacgagttg aacgccggca gcgacgagaa tgctgacggc gccatcgcgg
     1141 tcgatgcgtt gctggacgcg attccgagca gcgacggcga tccgagcggg atcgacgcgc
     1201 tcagccaccg gcttgctgag ctgaactcgg tcctggcggt ctccgacgat gagatcgagg
     1261 aagacgtcga tcaggatgtc gaccttgcct cggacgcagc gccgcatctc gaagtcgaga
     1321 cgacgaacta cgacatcggg gcgatcagcc tcgaggatcc cgtgcggatg tacctgcgcg
     1381 agatcggccg ggtgccgctc ctgaacgcac agcgtgaggt cgagctcgcg tcggcgatgg
     1441 agcgcggcga gtacctcaag gccaagcagc atcagctcac caccgatttc ggcgaggccc
     1501 ctgaggcaga tgtcctgggg cgagcgattt accactcgtt caagcagggt tggccgcacg
     1561 tgcaggcaat gtacgtggct gtccacggcg acgagcggtt gccggccaag tcgctgatgc
     1621 tcaagcgcgt gctgccgatg acccagctgc ccgaagaggc ggtctccgaa gtctgccaat
     1681 cccttggcat gacctcggac gagctcgagg agtcgatgcg cgttcgcagc gtcgagtggg
     1741 agctgctgcc cgtttcgatc cagaacctga tcagggatac gccggactgg ccggacgacg
     1801 ccgagatcga cgcgctcttc cgatcgcagg cggcccgact ccagcgccgc tgggacgaga
     1861 tgacccgcaa tggcgatctg gcaaaggtcg cgctgaccga ggccaatctg cgcctcgtcg
     1921 tctcggtcgc caagaagtac gccggccggg gcatgtcgat gctcgatctg gttcaggagg
     1981 gcaacctcgg tctgatccgg gcggtcgaga agtttcagca ccacaagggc ttcaaattct
     2041 cgacctacgc gacctggtgg atccggcagg cgattacgcg cgccatcgcg gaccaggccc
     2101 ggacgatccg aatcccggtc cacatggtcg agacgatcaa caagctgatc cggacctcgc
     2161 ggcggctcca gcaggagctc ggccgggagc ccacctcgga cgagatcgcc gtggcgatgg
     2221 agatgccgcc ggaacgagtg cgcgagatca tgaagatctc gcaggagccg gtgtcgctcg
     2281 agatgccgat tggcgaggaa gaggacagca acctcggcga cttcatcgag gaccagaagg
     2341 cactgccgcc ggccgatgcc gcgtcgcgca agatgctcaa ggagcaggtc gacgacgtgc
     2401 tcgggacgct ttccgagcgc gagcgggccg tgctggcgat gcggttcggc ctggatgatg
     2461 gtcgcagccg cacgctcgag gaggtcggcc gcgaattcgg cgtgacgcgc gagcggattc
     2521 gccagatcga ggccaaggcg ctccgcaagc ttcgtcaccc aagccgggcg aagaagctga
     2581 aggatttcct ggactgatca gtggacagaa aaaacggggc ggccaataag gccgccccgt
     2641 tccatgtata tgcgcattgc aaagcgcccg ccgctgcttg agccgttgaa atttgccaag
     2701 tgatcgtccg gaaacatagc gcggggtaac cacgccccgc ttggtgtctc ccacgcgcgg
     2761 atcatggggc cgaacgattg atggaattgg ttcccgcatc ggacgcccca tgatcgggtc
     2821 cgggcggggc gtggtaaccc cgcgctacgg acggaaagag atcccacgac tgcacgcgcg
     2881 actagaaccg gggagggccg gcttcgatac ggttgcggac ttcctggtac tgctgttcgc
     2941 ggtcgcccag gagctgccag cgggtttcga gccaccaggt cgcgccggca ttacgtagcg
     3001 gaccgatctt ctcctgcgct tcattggcat cgccgggaat cgtctcgcct tcgacgacga
     3061 aatcgaaccc ctcgccagcg ccgccccgtt cccgcagcca cgccagcatc gcgcggacat
     3121 cgtccggttc gacgctgcca tgctggccgt cgcgaatgat gtttggcagg acgccatcgc
     3181 agcgtaggac gcgggtcatc gacttttcgc tcggccatgc gccgacgacc cagatcggaa
     3241 tgtttgcctg gaccggctgc gcgacgtcgg aaaggtcggt ccggccggag aggtccatct
     3301 cgtagtgctg cccggaaaag actcgctcgc cgcgccagag ccccttgatc aggtcgatcc
     3361 cctcatccat catcgcggcc cgcgtcttgc ggtcccgctc ctcgccggtg ttgccgagga
     3421 agttgtcgac ggcgccgagt ccaaccgcca ggatcgcgcg tccattcgag agctgatcga
     3481 gcgtcacgac ctgactggcg accttccacg gccgtcgcca cgggagcggc gtcagcatcg
     3541 tgccaagctt gacccgctcg gtccgggccg ccattgcccc cagcagcgac caggcatcga
     3601 cgccataggc tgcttcccag acgaagacgg catcccagcc ggattgatcg accagcatcg
     3661 ccagctccag ctgctcgggc gcgctcccgc ccggcaacac aaacccaaac tgcatggtcc
     3721 ctccccctcg tctgttgttc ggtcgacggt tcgattgagt tggacacggt accatgagcg
     3781 cgatgtcagt accggtcctg aaatgaggtt ccatgtccga gcagtcgctc ccactggatg
     3841 acaccgtgct cttcgatccg gccgcgccgg gcgccatcga aatggcagcg gagctgatcg
     3901 cgcagggcgg cgtggtggcc atcccgaccg acacggtcta tggcgtggct gcctcgctct
     3961 cgcaccccga ggccctgcgc cgcatctacg agatcaaggg gcgggatcgt gccaagccgt
     4021 tgcccatcct cgtctcgtcg accgatgcgc tggcccggtt ggctccggag atcgatccga
     4081 acatcgcgct tctgctcaac gtctactggc ccgggccgct gacgatcgtg atcgagtcgt
     4141 cgcccggcat gccggccgaa gtgctcgcgt cggacgacac cgttgccgtt cgcctgccga
     4201 accacatgct tgccatcgag ttgatcgagc gggctggcgg cgcggtggcc tgcaccagcg
     4261 ccaacatcag cggccgcgac cccgccgcca gcgcgcagga cgtgctcgcc acggtcggtc
     4321 cggcgctgga cggcgtcgtc gagggtggga tcgcgccggg tggcgtgccg tcgaccatca
     4381 tccggatcgc ggacggggag gtcctgactg tgcgcgaggg accgatcccg atcgagcaca
     4441 tccgggcgac atgggacagc ttccgctccg aatgaaatct gcgcaatcat gtgaattcac
     4501 tgccaaaacc gagtaggatg ctgcgaggag gattgggcat ggtctccgat ttcggggaag
     4561 gcgtcacatc gcaggatcag acgatcgccg gggagcggaa ggatccgcgt cacgacccgg
     4621 cgaccaccgg cggcaccgtc ctgggtagcg tgcatccccc ggcgtacggt cgcgggttgc
     4681 tggtcagcat cgtcgcggcg gcgattgttt tcgcgctcgc gatctttgcg tccggcggca
     4741 aggaagaatt caccggcatc ccgatgaccc agacgaccgg ctcgctcttc tggggcttgt
     4801 cgatcctcgc gatcatcctc gtcggcgtgg cttcgcagta tgcggaagga gccgcgatca
     4861 gggttgccga atccctcggt caggaaccgg cccggcgcaa tcaattgccg tcggcgtggg
     4921 cggtcccggc ggcatcaatg gccaccgcgc tgctgcttgt cgcgacctat cacaacaagg
     4981 tcatgctcat cgccggtccg gccatcgcat ttctcggcgt ggcgggcggg ttgttcgcgc
     5041 gggatctgct cgaagacacg atggagggcg cggagcggac cgccgcgctg atccacacca
     5101 tcgtcatcca cgcgatcgcc ttcctcggac tgagcgcagt ctatatgaac aagatgagcg
     5161 gctggtatgg cggaccaatc gtcttcctgc tgacggccgt gctgatgttc gagcatctcg
     5221 agcgggcggg gcttccggac ggactccgcc tgctctattc gtccctctcg ggcggtgtga
     5281 tgatcctggc gctgctggcg gtcaactggt ggccgaccta cggctggacg ggcggcgcgg
     5341 ccctgttgct ctgcttcttc gtgcttgccg ggctcgggtc ggttcggggc gagcggcaat
     5401 cgctgcggga ccgcgacctg atcgaatacg gcgcggtctt cgtcggcggt ctcctgattc
     5461 ttgctattac cctctagccc gccaggaggc atcgtgtcga acgccggcag caagcgactt
     5521 ttcgacctct acgagcggat ggaccggctc gaggagctga tcgaggagat ggaggagctc
     5581 ggcatcagca cgcgcgaaga ggccgagcag atgatcgtcg agctcgacgg tcagatcgag
     5641 gagctggaat cgcacgacga ttccggtgat tgagttttcc tatgacgctc gatatgttcg
     5701 aatcccgccg caaggaccaa ctggcggttc atgcgcccct tgccacccgg atgcgaccgc
     5761 gcacgctcga cgacctcgtc ggtcatgaag acgtggtcgg acccgggatg ctgctccgca
     5821 aggcgatcga tgcc
//
LOCUS       DATAOZ010000041         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5807794, whole genome shotgun sequence.
ACCESSION   DATAOZ010000041 DATAOZ010000000
VERSION     DATAOZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5807794"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..99)
                     /locus_tag="VHR64_01190"
     tRNA            complement(27..99)
                     /locus_tag="VHR64_01190"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(64..66),aa:Phe,seq:gaa)
     gene            complement(120..195)
                     /locus_tag="VHR64_01195"
     tRNA            complement(120..195)
                     /locus_tag="VHR64_01195"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Val,seq:tac)
     gene            complement(213..297)
                     /locus_tag="VHR64_01200"
     tRNA            complement(213..297)
                     /locus_tag="VHR64_01200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Leu,seq:gag)
     gene            525..1484
                     /gene="mdh"
                     /locus_tag="VHR64_01205"
     CDS             525..1484
                     /gene="mdh"
                     /locus_tag="VHR64_01205"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX3301470.1"
                     /translation="MSSASSGRRKVTVIGAGMVGGTVAQMLAIRDYADVVLVDIVEGM
                     PQGKSLDLMQMGTLLGFDTQLTGANSYDETAGSDVIVITSGIARKPGMSRDDLLKTNM
                     GIVESVTKQVVSSSPDAVIIVVSTPLDAMCHVAFDAAGFRRERVIGMAGVLDSARFRA
                     FIAMEMGVSVEDVSAMVLGGHGDTMVPLSRFSTVGGVPITELLAADRVSAIEERTAKG
                     GAEIVGLLKTGSAYYAPGMSTVEMVDAIMLDKKRVLPCSVLLKGEYGISDLYVGVPIK
                     LGAGGVEQIIELNLTGDESAALKKSSGAVKELVDAMASIRASA"
     gene            1500..2438
                     /locus_tag="VHR64_01210"
     CDS             1500..2438
                     /locus_tag="VHR64_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301471.1"
                     /translation="MPFGPGKKNDQGKGGKVAGARQLVSLARGVSIEEIEQAVRQPPR
                     ILFVSAEPDIDDLVSELTGMRGMPTLEVVSPDSVPRDLGQYDVIVVHSRLSNADYLNV
                     RKIAGVASHRVFDAGQAVDINELRTRIVEHAGNGAAALGRWYPGFRDAAATAIINETS
                     RANAQFTLMTAVPSAIPVIGSFAMAGADMIVLTKNQLLMAIKLAAVHGKPFGDKKAIL
                     KDLMPVIGSGFVWRSLAREAVVFLPFAAGTIPRVAIAFAGTYTTGRAVDAYYRFGKKP
                     TKTQLNDYYQQAMALVKARFAKPRAVAEPETAAPLG"
     gene            complement(2435..>2806)
                     /locus_tag="VHR64_01215"
     CDS             complement(2435..>2806)
                     /locus_tag="VHR64_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX3301472.1"
                     /translation="ATLEAIAMQVADVLRAMVSDAGSPVTELKVDGGAAGNDLLLQLQ
                     ADLLGVSVVRSAIQETTALGAAYLAGLGIGYWADESELSRMWQSNGQFAPAISADERE
                     SRIAIWRRAVERSRGWANVGG"
BASE COUNT          539 a          891 c          879 g          497 t
ORIGIN      
        1 gagcctgatt gaggctcagg cgcaggtgcc aagggccgga ctcgaaccgg cgaccgcacg
       61 attttcagtc gtgagctcta ccaactgagc taccttggca gtggatccgt gtcgcatgct
      121 ggtgggcgat agagggctcg aacctctgac atccacggtg taaacgtggc gctctaccag
      181 ctgagctaat cgcccacgga taggttttaa ggtgcccagg aagggatttg aacccccacg
      241 cccgtaatgg gcactagccc ctcaagctag cgcgtctgcc gattccgcca cctgggcaag
      301 cggccacaaa ggccagcaga ttatgctgag atcgcgtctc cgtgtcaacc gagacacagg
      361 caattttacc gcgatcagga cttctcgccc aatcgggacg ctccacggtc attcgttgtt
      421 tgcagcccag cgcagtccgt cccgggccct tacctgccta tactaccgac gttggttggc
      481 acggtaccgg ggacgtttcc ccgttgattg caggagttcc actgatgtca tcggcttcgt
      541 caggtcgtcg gaaagttacc gtcatcggcg cgggcatggt cggcggtacc gtcgcccaga
      601 tgctcgcgat ccgcgactac gcggacgtcg tcctggtgga cattgtcgaa gggatgccgc
      661 agggcaagtc gctcgacctg atgcagatgg gcacgctgct cggcttcgac acgcaactga
      721 ccggcgccaa cagctacgac gagacggccg gatcggatgt gatcgtgatc acatcgggca
      781 ttgcccgcaa gccgggtatg agccgggacg atttgctcaa gacgaatatg ggcatcgtcg
      841 aatcagtcac gaagcaggtc gtctcaagct cgcctgacgc ggtcatcatc gtcgtctcga
      901 ctccgctcga cgcgatgtgc catgtcgcct tcgacgcggc cggtttccgg cgcgagcggg
      961 tgatcggcat ggccggggtg ctcgactccg cgcggttccg cgccttcatc gcgatggaga
     1021 tgggcgtctc tgtcgaggat gtaagcgcaa tggttctcgg cggccacggc gacaccatgg
     1081 tgccgctctc ccgcttttcg accgtcggcg gcgtgccgat cacggaactc ctcgcggcgg
     1141 accgggtgtc ggcgatcgag gagcggacgg ccaaaggcgg tgcggaaatc gtcggcctgc
     1201 tcaaaaccgg cagcgcctat tacgcgcccg ggatgtcgac cgtcgagatg gtcgacgcga
     1261 tcatgctcga caaaaagcgg gttctcccct gcagcgtcct gctcaagggc gaatacggca
     1321 tcagcgacct ctatgtcggc gtcccaatca agctgggagc gggtggtgtc gagcaaatca
     1381 tcgagctgaa tttgaccggc gacgagtcgg ccgcgctcaa gaagtcgtcc ggagccgtca
     1441 aggaactggt cgacgcgatg gcctcgattc gcgcatcggc gtagcagcga ggagcgacag
     1501 tgccattcgg accggggaaa aagaacgatc aaggcaaggg cggcaaggtg gccggagccc
     1561 gacagctcgt gtccctggca cgtggcgtca gcatcgagga gatcgagcaa gcagttcgac
     1621 agccaccacg aatcctgttt gtcagtgccg agccagatat cgacgatttg gtctccgagt
     1681 tgacgggcat gcgcggaatg ccgacgctcg aggtcgtcag cccggacagt gtccctaggg
     1741 acctcggtca atacgatgta atcgtggtcc acagccgatt atccaatgcc gactacctga
     1801 acgtgcggaa aatcgccggc gtcgccagcc atcgagtatt cgacgccggg caggcggtcg
     1861 acatcaatga gttgcggacc cgaatcgtcg aacacgcagg caatggcgcc gccgcgctgg
     1921 gcaggtggta tccaggattc cgcgatgcgg ccgcgaccgc gatcatcaac gagacctcgc
     1981 gggcgaacgc gcagttcacc ctcatgaccg ctgtcccgag cgccattccg gtgatcggct
     2041 catttgcgat ggccggcgcg gacatgatcg tcctgaccaa gaaccaactg ctgatggcga
     2101 tcaagctggc agcggtgcac ggcaagccgt tcggggacaa aaaggcgatc ctcaaggacc
     2161 tgatgccggt tatcggctcc ggattcgtct ggagatcact cgcgcgggag gcagtcgttt
     2221 tcctgccgtt cgcggccgga accattccga gggtcgccat cgcctttgcg ggcacctaca
     2281 cgacgggccg ggcagtcgac gcctattacc gattcggcaa gaagccgacg aagacccagc
     2341 tcaatgacta ctaccagcag gcaatggcgc tggtgaaggc ccgcttcgcc aagccgaggg
     2401 ctgtagccga accggagacc gctgccccgc tcggttaacc gccgacattc gcccagccgc
     2461 gtgaccgctc gaccgcacgc cgccagatgg caattctcga ttcccgctca tcggccgaga
     2521 tcgccggcgc gaattggccg ttgctctgcc acatccgact cagctccgac tcgtccgccc
     2581 agtaaccgat gccgagtccg gccagatagg cagcgccgag cgccgtcgtc tcctggattg
     2641 ccgatctgac caccgacacg ccgagcaggt ccgcctggag ctgcaggagc aggtcattgc
     2701 cggccgcgcc cccatccact ttcaactcgg tcaccggcga accggcgtcg ctcaccatcg
     2761 cgcggaggac atcggccact tgcatcgcga tcgcctcaag cgttgc
//
LOCUS       DATAOZ010000042         5366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9228292, whole genome shotgun sequence.
ACCESSION   DATAOZ010000042 DATAOZ010000000
VERSION     DATAOZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9228292"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..114
                     /locus_tag="VHR64_01220"
     CDS             13..114
                     /locus_tag="VHR64_01220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX3301473.1"
                     /translation="MSNDLTLTLNKARQAQITREVSEIAAGAEALKG"
     gene            124..1524
                     /gene="atpD"
                     /locus_tag="VHR64_01225"
     CDS             124..1524
                     /gene="atpD"
                     /locus_tag="VHR64_01225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX3301474.1"
                     /translation="MVATAAATGRIVQVMGPVVDIEFPPDQLPEIYNALEVKMDDETT
                     LVLETQTHLGNDWVRAVAMSTTDGLRRGMNAVDTGQAISVPVGPETLGRILNVVGAPI
                     DQAGPVEAKTIYPIHRPAPSFEEQSTSVEIFETGIKVIDLIAPFTKGGKTGVFGGAGV
                     GKTVIITELIRNIASEHSGYSVFTGVGERTREGTALWGEMQASGVANQTVLVFGQMNE
                     PPGARLRVGLTGLTMAEYFRDEGRDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQP
                     TLASEMGQLQERITSTKTGSITSVQAVYVPADDYTDPAPATTFAHLDATISLERAIAE
                     RGIYPAVDPLASTSRILDPLIVGDEHYRVAREVQQVLQRYRDLQDIIAILGIEELGEE
                     DKQVVARARRIERFFSQPFFTAEQFTGTPGKYVTREETVRAFKEILEGKYDSLPESAF
                     MYRGGIDEVVAAAQGQ"
     gene            1621..2076
                     /locus_tag="VHR64_01230"
     CDS             1621..2076
                     /locus_tag="VHR64_01230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX3301475.1"
                     /translation="MAKLDVEIVTGERVVLSETDVDMVVAPGSDGSLGILPQHAPLIS
                     TLSAGELRVKKGGREQSIVVFGGFIEVTPHKVIILADTAERIEDIDVGRAEEARRRAE
                     DAIRNRGEAVEIEDALAALRRANLRLNVGRRHRRPGAGPGVATSSDNSV"
     gene            2175..3542
                     /gene="murA"
                     /locus_tag="VHR64_01235"
     CDS             2175..3542
                     /gene="murA"
                     /locus_tag="VHR64_01235"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX3301476.1"
                     /translation="MVDIAVHTGMTPDLPLTGSPTRALWIEGGAPLRGAVQIGGAKNA
                     ALPLLAATLLTGEECTLTNMPDLSDIHTMVELLRALGADVEFDTKKHRVRVQAANIST
                     TSAPPELVAKMRASFLVAGPLLARFNETTASTPGGCKLGARPVDVDVRGFRKMGAEVQ
                     ATEQMINAKTVGLHGARIYMDYPSHTGTENLLMAAVLAVGKTTIVNAACEPEIVALGN
                     MLNRMGAQISGLGSPTIVVQGVDRLHGVSDMILPDRLEAGCYAIGAVITKGEVTLQGV
                     REPDMLPLTEKLREAGAEVWHQESDMLVRTRQQLQAVEIQTLPFPGFPTDLQAPFAVL
                     MTQSAGLSRIHERVFDDRLRYADELLKMGADIRVEKFAPNRYGTKADIIGPTPLTGAS
                     VRALDIRAGAGMVLAGLVANGRTTVSDVYHLDRGYEGLVPKLRQIGAQITEVGYDSPA
                     DGAAK"
     gene            3560..4048
                     /locus_tag="VHR64_01240"
     CDS             3560..4048
                     /locus_tag="VHR64_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3301477.1"
                     /translation="MDSIDSVDTELMYDGIPLGARDDRADVSTQLMADQRSGQRILIV
                     DDEAAVADLIEAVLVGEGYTVAIARDGVQGILLARDWKPDLVLMDIMLPGIDGTTAIR
                     RLKSDPATAELPIVAMSAGRTIRRQSNELADADAALAKPFDIEALLAQIEFLLSRRRA
                     EQ"
     gene            4115..4822
                     /locus_tag="VHR64_01245"
     CDS             4115..4822
                     /locus_tag="VHR64_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX3301478.1"
                     /translation="MTRSITIVIPTYNERANLETIITELVKLLPEAFVVVVDDNSPDG
                     TGALADDLVCRFPGVQVIHRPSKEGIGPAYVAGFKRALAMGTDLVAAMDADGSHAPTD
                     LLRLIEAAENADLVVGSRYVDGGETAGWPRSRRLLSRFGGIYARWVLGAPIADLTAGF
                     KVYRRDALASLPLDQIRTDGYGFQIETTWDIWRSGQRVCEVPITFHDRVAGKSKLSRR
                     IVIEAMIMVWRLRFSGR"
     gene            complement(4788..>5366)
                     /locus_tag="VHR64_01250"
     CDS             complement(4788..>5366)
                     /locus_tag="VHR64_01250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3301479.1"
                     /translation="NPPIDVTQFEPEVQPPPEDWYLVVSRLVPHKRIDLVVETASAHN
                     IPVKIIGTGRDLDRLRAMAGPTIEFLGFRPHAEVIDHYQRCRAFILPGVEDFGMTAVE
                     AQASGRPVIALGRGGALETIQDGTTGILFDAQTPESLLAAMQRLEELAISGGACVCNA
                     RRFDESVFREGIQRAVERALAITGQRSAIARP"
BASE COUNT          972 a         1776 c         1693 g          925 t
ORIGIN      
        1 aacgccaagg atctgtccaa cgatctgaca ttgacattga acaaggcacg ccaggcccag
       61 atcacccgcg aggtgtcgga gatcgcagcc ggtgcagagg ccctcaaggg ctaggagagg
      121 cacatggtag ctacagcagc agcaaccggc cgcatcgtgc aagtgatggg cccggtcgtc
      181 gacatcgagt tcccgccgga ccagcttccg gagatctaca acgcgctcga agtcaagatg
      241 gacgacgaga cgacgctcgt gctcgagacg cagacgcacc tcggcaatga ctgggtccgc
      301 gccgtcgcca tgagcacgac cgatggcctg cgtcgcggca tgaacgccgt cgacaccggc
      361 caggcgattt ccgtgccggt cggtccggag acgctcgggc gcatcctgaa cgtggttggc
      421 gctccgatcg accaggcagg cccggtcgag gccaagacga tctatccgat tcaccgcccg
      481 gctccatcgt tcgaggagca gtcgacctcg gtcgagatct tcgagacggg gatcaaggta
      541 atcgacctga tcgccccgtt cacgaagggc ggcaagaccg gcgtcttcgg tggcgccggc
      601 gtcggcaaga cggtcatcat caccgagctg atccggaata ttgcctccga gcactccggc
      661 tattcggtct tcaccggcgt aggcgagcgg acccgcgagg gcacggcgct ctggggcgaa
      721 atgcaggctt cgggcgtcgc caaccagacg gtcctcgtct tcggccagat gaacgagccg
      781 ccgggagccc gcctccgcgt cggtctgacc ggtttgacca tggctgagta cttccgcgac
      841 gaggggcgcg acgtgctcct cttcatcgac aacatcttcc gcttcgtcca ggcgggctcg
      901 gaagtctccg ccctcctcgg ccgaatgccg agcgccgtgg gttaccagcc gacgcttgcg
      961 tcggagatgg gccagctcca ggagcggatt acgtcgacca agaccggctc gatcacctca
     1021 gtgcaggcgg tctacgtgcc ggcggacgac tacaccgacc cggcgccggc cacgaccttc
     1081 gcccacctcg acgcgacgat ctcgctcgag cgggcgatcg ccgagcgtgg catctacccg
     1141 gcggtggacc cgctcgcttc gacctcccgc atcctcgatc cgctgatcgt gggagacgag
     1201 cactatcgcg tagcccgcga ggtccagcag gtgctccagc ggtatcgcga cctccaggac
     1261 atcatcgcga ttcttggtat cgaagagctc ggcgaagagg acaagcaagt ggttgcccgc
     1321 gcccgccgca tcgagcgctt cttctcgcag ccgttcttca cggctgagca gttcaccggt
     1381 acgccgggga aatacgtgac tcgcgaagag acggtccgcg ccttcaagga gatcctcgag
     1441 ggcaagtacg acagccttcc ggagtcggcg ttcatgtacc gcggtgggat cgacgaagtc
     1501 gttgccgccg cgcagggtca atagtcatct tcaggccata gcggggcgtt cctgccccgt
     1561 aatggcccgc ttttcgcccc agtccgcacc cgatgcaagg gctaacgagg aacggcatcc
     1621 atggccaagc ttgacgtaga gatcgtaacg ggcgagcgcg tcgtcctttc tgagacggat
     1681 gtcgacatgg tcgtcgcacc cggttctgat ggttcgctcg gtatcctgcc gcagcacgct
     1741 cccctcattt ccaccctttc cgcgggtgag ctgcgcgtca aaaaaggtgg ccgggagcaa
     1801 tccatcgtcg tcttcggcgg ctttatcgaa gtgacgccgc acaaggtcat catcctggct
     1861 gacacggccg agcgcatcga agacatcgat gtcggccgtg ccgaggaagc ccgccgccgc
     1921 gccgaagatg cgatccggaa tcgcggcgaa gcggtcgaga tcgaagacgc gcttgccgcc
     1981 cttcgccgcg cgaacctgcg cctgaacgtc gggcgccggc atcgccgccc gggggctggg
     2041 cccggcgtgg ccacgagcag cgacaattcg gtatagtctc cccacaatcc gaccagcgac
     2101 gatcatgtcg aggagagacg tacgctccgt gccttctgag cgctcccacg tgacggcgca
     2161 caggagttac gacagtggtc gacatcgccg tgcataccgg catgacacct gatttgcccc
     2221 ttactggatc acctacgcgg gccctttgga tcgagggtgg cgccccattg cggggtgccg
     2281 tccagatcgg cggcgcaaag aatgccgccc ttccgctgct tgcggccacg ctgctgaccg
     2341 gcgaggaatg cacgctcacc aacatgccgg acctctcgga catccacacg atggtcgagc
     2401 tgctgcgagc gctcggtgcc gacgtcgagt tcgacacgaa gaagcaccgg gtgcgggtgc
     2461 aggcggcgaa tatctcgacc acgtcggccc cgccggagct cgtcgccaaa atgcgcgcct
     2521 cgttccttgt cgccggtccg ctgctcgccc ggttcaacga gacgaccgcc tcaacacccg
     2581 gtggatgcaa actcggtgcg cgaccggtcg atgtcgatgt ccgcgggttt cgcaagatgg
     2641 gcgcggaggt ccaggccacc gagcagatga tcaacgcgaa gaccgtcggc ctgcacggcg
     2701 cccggatcta catggactac ccgagccaca ccggtaccga gaacttgctc atggccgccg
     2761 tccttgcggt cggcaagacg acaatcgtca atgcggcctg cgagccggaa atcgttgccc
     2821 tgggcaacat gctcaatcgg atgggcgcgc aaatctccgg actgggctcg ccgacgatcg
     2881 tggtgcaggg cgtcgatcgg ttgcacgggg tgtccgacat gatcctgccg gatcgcctcg
     2941 aagccggctg ctacgcgatc ggcgcggtga tcaccaaggg cgaggtcaca ctccagggcg
     3001 tgcgcgagcc ggacatgctg ccgctgacgg agaagctgcg cgaagccggc gccgaggtct
     3061 ggcaccagga aagcgacatg ctcgtccgca cgcggcagca actgcaggcg gttgagattc
     3121 agacgctgcc cttccccggc tttccgaccg acctgcaggc gccgttcgcg gtgctgatga
     3181 cccagtccgc cggcctctcc cggatccatg agcgcgtctt cgacgatcgc ctgcgctatg
     3241 ccgacgagtt gctcaaaatg ggcgccgaca ttcgggtcga gaagttcgcg cccaatcgct
     3301 acggcacaaa agcggatatt attggcccca caccactcac gggcgccagt gttcgggcgc
     3361 tcgacattcg agcaggagcg ggtatggtgt tggccggcct tgtcgccaac ggtcggacaa
     3421 ccgtttcaga cgtctaccac ctcgatcgcg ggtacgaggg tctcgtgccg aaacttcgcc
     3481 aaatcggagc gcagatcacc gaggttggat acgactctcc ggcggacggc gccgccaaat
     3541 gatggcaggg ggactgagaa tggattcgat cgattccgta gacactgagc tcatgtacga
     3601 cgggattccg ctcggtgcga gagacgatcg agccgatgtg tcgacgcagc tgatggccga
     3661 ccagcgctcc ggccaacgca tcctgatcgt cgacgacgag gccgccgtgg ccgacctgat
     3721 tgaggccgtg ctggtcggcg agggctacac cgtcgccatc gcccgcgacg gcgtgcaagg
     3781 tatcctcctc gcgcgcgact ggaagccaga tctcgtcctg atggacatca tgctgcccgg
     3841 tattgacggc accaccgcaa tccgccgcct caagagcgac ccggcgaccg cagagttgcc
     3901 gatcgtcgcc atgtcggctg gtcgaaccat tcgccgccag tcgaacgagc tggccgacgc
     3961 cgacgcggca ctcgccaagc ccttcgacat cgaggcgctg ctcgcccaga tcgagttcct
     4021 gctctctcgc cgccgagccg agcaatagcg caatcagcat cccactgatc ttcccgcaga
     4081 cgggcgtcgc accgtcgtat ttcggagtgc cagcgtgacc agatcgatca cgatcgtcat
     4141 tccgacctac aacgagcgcg caaacctcga gacgatcatc actgagctcg tcaagttgct
     4201 cccggaggca tttgttgtcg tggtcgacga caattcgccc gacggcaccg gcgccctggc
     4261 agacgatctc gtgtgccgct ttcccggcgt ccaggtgatc caccgcccgt ccaaggaggg
     4321 gatcggtccg gcctacgttg ccggtttcaa acgcgcgctg gcgatgggta ccgacctcgt
     4381 cgcagccatg gacgccgatg gatcccacgc ccccaccgac ctgctccgct tgatcgaggc
     4441 ggctgaaaat gccgacctgg tcgtcggttc ccgctatgtg gacggtggtg agaccgccgg
     4501 gtggccgcgg tcccggcgat tgctgtcgcg gtttggcggg atctatgcgc gctgggtcct
     4561 cggcgcaccg atcgcggatc tcacggccgg tttcaaggtc taccggcgcg atgcgctcgc
     4621 gtcgctccca ctcgaccaga tccggactga cggttacggg ttccagattg agacgacatg
     4681 ggacatttgg cgatcgggcc agcgtgtgtg cgaggttccg atcacgtttc acgatcgcgt
     4741 cgccggcaag tccaagctct cgcggcggat cgtgatcgag gcgatgatta tggtctggcg
     4801 attgcgcttc tctggccggt aattgccagc gcccgctcaa cggcgcgctg gatcccctcg
     4861 cggaaaaccg actcgtcgaa tcggcgggcg ttgcaaacgc aggccccacc gctgatcgcg
     4921 agctcctcaa gtcgctgcat ggccgccagc aacgattccg gcgtctgggc atcgaacagg
     4981 atgcccgtag tgccgtcctg gatcgtctca agtgccccgc cccgcccgag tgctattacg
     5041 ggccgtcccg aggcttgcgc ctcgacagcg gtcattccga aatcctcaac gccgggaaga
     5101 atgaatgccc ggcaacgttg gtagtggtca atcacctctg catgcgggcg aaagcccaga
     5161 aactcaattg tcggtccggc catcgctcgc aaccgatcaa ggtcgcgccc ggtaccgatg
     5221 atcttgaccg ggatgttatg cgccgacgcc gtttccacca cgagatcgat ccgtttgtgc
     5281 ggcacaagtc gggacacgac caggtaccaa tcctccggcg gcggctggac ctcgggctcg
     5341 aactgcgtga catcgatcgg cggatt
//
LOCUS       DATAOZ010000043         4416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8701961, whole genome shotgun sequence.
ACCESSION   DATAOZ010000043 DATAOZ010000000
VERSION     DATAOZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8701961"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VHR64_01255"
     CDS             <1..753
                     /locus_tag="VHR64_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3301480.1"
                     /translation="FGISFNKRYRADVELLKAQAKAGALGEIYYAKAFWMRRAGIPGL
                     GSWFTSKNLAGGGSLIDLGVHVIDMVLWLMGNPTISTVSAATYSRLGPKGRGHWAGHR
                     FQHNPDSVFEVDDLATAFLRTDKGAVIHLETSWASYSSHTDDHGVILMGDEGGAEIRI
                     HDYQKTGALKFFTDMNGVPVDTQPRTIVQHEHQQIIKRFIDAITLGVPMTPSGQEGVD
                     RARIIDSIYESAALGHEITINQPVQTAATAAD"
     gene            765..1490
                     /locus_tag="VHR64_01260"
     CDS             765..1490
                     /locus_tag="VHR64_01260"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX3301481.1"
                     /translation="MTLRVTVWNEFRHEKQNEQIGAIYPDGMHTAIANRLGKETDFEV
                     RTATLDEPEHGLTQAVLDSTDVLTWWGHKAHAEVSDEVVERVRERVLDGMGLIVLHSA
                     HYSKIFKTLMGTSCSLKWREANDNERIWIVNPAHQITRGLPEFIDIEREEMYGEYFDI
                     PAPDELLMVSWFKGGEVFRSGCTFTRGRGKIFYFRPGHESYPTYHNEQVLHIIINGVR
                     WAAPSGGPAPTFHNYQPLESLEG"
     gene            1494..2621
                     /gene="solA"
                     /locus_tag="VHR64_01265"
     CDS             1494..2621
                     /gene="solA"
                     /locus_tag="VHR64_01265"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEX3301482.1"
                     /translation="MAHYDAIVIGGGTMGTAAAWELGKRGLKALVLEQFEHVHTKGAH
                     SGETRIIRHAYAEGPDYVPLVFRADDLWMELEALAGELLFHRVGAIELSAPGNNHAAL
                     ARESAIEHGIPFEWLAMDDVRSRYPQFAVGDDWVGGFGDRGGFLDVERSLRAMASQAR
                     SHGVEIREHAKVEGWSASDSAVTVSVDGSTETADRLIITAGSWTNQVLGDAALPLRVL
                     RKTLFWLEVDDPARYTSDVLPVYIIGLPHHEFYGFPIWGRPGIKVAIHSGGDETNPDS
                     VDREISAAERDEIVEVARIALHGVTGRVLHEATCLYTVSPDHDFIVDFALGTSNVVIG
                     AGFSGHGFKFTPAIGELLVQMLYGEREPLPLLSRNRFVGAV"
     gene            complement(2618..2947)
                     /locus_tag="VHR64_01270"
     CDS             complement(2618..2947)
                     /locus_tag="VHR64_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301483.1"
                     /translation="MVQIYPGQAYDHQLADKTVAFLQTRDPAIFPFTREFDEERMRAF
                     LRDLREGLADLTDSGSARKTSSTGFMMRDRRLRETVEALAVADGDWPVGSDPRVPGRG
                     LGTPGPL"
     gene            3086..>4416
                     /locus_tag="VHR64_01275"
     CDS             3086..>4416
                     /locus_tag="VHR64_01275"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX3301484.1"
                     /translation="MADIENLRRSTLENGLDVLLYPSFDAPAASFWVFYRVGSRNELP
                     GITGVSHWVEHMMFKGTPKVGPGELMLRVNRNGGELNAFTSYDYTAYHQTLPADRIGL
                     AVDLEADRMVNLLIDPGETDSERTVILSERQGAFNNPSYVLWDETVATAFRAHSYREF
                     VIGSEHDLKTMSRDDLYGYYRRFYAPNNAVVVVSGAFDADAMQAEIERAFGSIPASDG
                     IDQRVAVEPAQIAERRVELLHPAPAPEVLMGFKVPGAGDPDTRALELLAAVLSGAGGR
                     MGKSSRLPRALVATGKARSASATYLKGIDPFLFILGATGLPDGDAHDLDRLLMEQLTL
                     IKEKGITQADLDRAKKQLITSFHYGSESISEQAHGIGDAAMYGKSDDFFTYPDDIAAV
                     TLEGVKQVVAEYFRPSNRTVGYLVPTEPASGGPAVDGVAALRFGLGGVGAPA"
BASE COUNT          811 a         1388 c         1427 g          790 t
ORIGIN      
        1 ttcgggatct cgttcaacaa gcggtaccgg gccgatgtcg agttgctcaa ggcacaggcc
       61 aaggccggcg ccctgggcga gatctactac gccaaggcct tctggatgcg ccgcgccgga
      121 attcctggtc tcggaagttg gttcacgagc aagaacctcg ccggcggcgg ctcgctgatc
      181 gaccttggtg tgcacgtgat cgacatggtc ctttggctga tgggcaatcc gacgatctcg
      241 acggtctcgg cggcgaccta ctcccggctc ggaccgaagg gacgcggcca ctgggccggt
      301 catcgctttc agcacaaccc ggacagcgta ttcgaggtcg acgacctggc gacggcgttc
      361 ctgcgcaccg acaagggcgc ggttatccat ctcgagacaa gctgggcgtc gtacagctcg
      421 cacaccgacg atcacggcgt gattctgatg ggtgacgagg gtggtgccga aatccggatc
      481 cacgattacc agaagacggg cgcgctcaag ttctttaccg acatgaacgg tgtcccggtc
      541 gacacccaac cgcgcacgat cgtccagcat gagcatcagc agatcatcaa acggttcatc
      601 gacgcgataa cgctcggtgt accgatgact ccgagcggcc aggagggtgt tgatcgcgcc
      661 cggatcatcg actcgatcta cgagtcggcc gcgctcggac acgagatcac gatcaatcag
      721 ccagttcaaa ccgctgccac cgcggcggat taggagcctc atacgtgacg ttgcgagtta
      781 cggtctggaa cgagttccgg cacgagaagc agaatgagca aatcggggcg atctaccccg
      841 atggcatgca tacggcaatc gcgaatcgtc ttggcaagga aaccgacttc gaggtgcgga
      901 ccgccacgct cgatgagccg gagcacggcc tgacgcaggc ggtcctcgat tcgaccgacg
      961 tcctgacttg gtggggacac aaggcgcacg ccgaggtgtc ggacgaggtg gtcgagcggg
     1021 tccgggagcg ggtgctggat gggatgggcc tgatcgtcct ccactccgcc cactactcga
     1081 agatcttcaa gacgctgatg ggcacaagct gcagcttgaa gtggcgcgag gccaacgaca
     1141 acgagcggat ctggatcgtc aatccggctc accagatcac gcggggcctg ccagagttca
     1201 tcgacatcga gcgggaggag atgtacggcg agtacttcga catccccgcg cccgatgagc
     1261 tgctgatggt gagctggttc aagggcggcg aggtcttccg cagcggctgc accttcaccc
     1321 gcggccgcgg caagatcttc tacttccggc cgggccatga gtcgtacccg acctaccaca
     1381 acgagcaggt gctgcacatc atcatcaatg gcgtgcgctg ggccgctccg tccggcggac
     1441 ccgcgccgac attccacaac taccagccgc tcgaatccct cgagggctga cgagtggctc
     1501 actacgacgc gatcgtgatc ggcggcggga ccatgggcac cgccgccgcc tgggagctcg
     1561 gcaagcgcgg attgaaggcg ctggtgctcg agcagttcga gcatgtccac accaagggcg
     1621 cgcacagcgg cgagacccgg atcatccggc atgcatacgc cgagggtccc gattacgtcc
     1681 cgctcgtctt ccgcgccgac gacctctgga tggagctcga ggcgttggcg ggagagctgc
     1741 tcttccaccg agtcggcgcg atcgagcttt cggcgcccgg gaataaccac gccgcactgg
     1801 ctcgggaatc ggccatcgag cacggcatcc cgttcgagtg gctcgcgatg gacgatgtcc
     1861 gcagccgcta cccgcagttt gccgttggcg atgactgggt cggcggtttc ggcgatcgtg
     1921 gcggcttcct cgatgtcgag cggagcctcc gggcgatggc aagtcaggcg cgctcccacg
     1981 gtgtcgagat ccgcgagcat gcgaaggtcg aaggctggtc ggcgtccgac tccgccgtga
     2041 ctgtttcggt cgacggctcg actgagacgg ctgaccggct gatcatcacc gccggcagct
     2101 ggacgaatca agtgctgggc gatgccgcgc tcccactgcg tgtcctgcgc aagacacttt
     2161 tctggctcga agtcgacgat ccggcccgct acacgtcgga cgtgctgccg gtctacatta
     2221 tcggcctgcc ccatcatgaa ttctatggct tcccgatctg gggaaggccg gggatcaagg
     2281 tagcgatcca ctccggcggc gatgaaacta acccggatag cgtcgatcgc gagatatcag
     2341 cggccgagcg ggacgagatc gtcgaggttg cccgcattgc gctgcacggc gttaccggtc
     2401 gagtcctgca tgaagcgacc tgcctctaca cggtctcgcc ggatcatgat ttcatcgtcg
     2461 atttcgcact gggaacgagt aatgtcgtga tcggcgccgg tttctcaggg cacggattca
     2521 agttcacgcc ggccatcggc gagctgctcg tgcaaatgtt gtacggcgag cgggaaccgc
     2581 tgccgctgct cagtcgcaat cgattcgtgg gagcagtcta gagcggaccg ggggtaccga
     2641 ggccgcggcc gggcacacga ggatcagagc caaccggcca atcgccatcg gcaactgcca
     2701 aggcctcgac cgtctcgcgc aggcggcgat cccgcatcat gaagccggtt gacgaggtct
     2761 tgcgtgcgct cccgctatcg gtcaggtcgg ccaggccttc tcgcaggtcg cgcaaaaagg
     2821 cgcgcatccg ctcttcatcg aactctcgcg taaacgggaa gatcgcggga tcgcgggttt
     2881 gcaggaacgc gacggttttg tcggccagtt ggtgatcgta ggcctgcccc ggatagatct
     2941 gaaccacgcc gtgcccccgt agctaaaccg tcgcccgcct tgccattgcc gggccctgcg
     3001 cggatattgt agcgatgatc cggcgactga cgcgcacgca tgcagcacgg tcgcatggcg
     3061 cttaccgcta tccagggagt tgcacgtggc agatatcgaa aaccttcgtc gatcgacgct
     3121 cgaaaatggg ctcgatgtgt tgctttaccc gagcttcgat gccccggccg cctcattctg
     3181 ggtcttctac cgggtcggct cgcgcaacga gctgccgggg attaccggcg tctcgcactg
     3241 ggtcgagcac atgatgttca aggggacgcc gaaggtcggc cccggcgagc tgatgctgcg
     3301 cgtcaatcga aacggcggcg agctgaacgc cttcacctcg tacgactaca ccgcctacca
     3361 ccagaccctc cctgcggatc ggatcgggct ggccgtcgat cttgaggcgg accgcatggt
     3421 caacctgctg atcgatccgg gcgagaccga ctcggaacga accgtgatcc tttccgagcg
     3481 gcagggtgcc ttcaacaacc cgtcatacgt gctctgggat gagaccgtcg ccactgcctt
     3541 ccgggcgcac agctaccggg agttcgtgat cggctctgag cacgatctca agacgatgag
     3601 ccgggacgat ctctatggct actaccgccg cttctacgcg ccgaacaatg ccgttgtggt
     3661 cgtgtcaggg gcgttcgatg ccgatgcgat gcaggccgag atcgagcgcg catttgggtc
     3721 gatcccggcc tcggacggca tcgatcagcg cgtcgccgtc gagccagctc agatcgccga
     3781 gcgacgcgtc gagctgctgc atccggctcc ggccccagaa gtgcttatgg gattcaaggt
     3841 acccggcgcc ggcgatccgg acacgcgcgc gctggaattg ctggcggcgg tgctctccgg
     3901 ggcaggcggc aggatgggca agtcatcccg attgccgcgt gcgttggtgg cgaccggcaa
     3961 ggcacgctcg gccagtgcga cctacctgaa aggcatcgac ccattcctct tcatcctcgg
     4021 cgcgaccgga ttgccggacg gcgatgccca cgatctcgat cgtttgctca tggagcaatt
     4081 aacgctgatc aaggagaagg gcatcaccca agccgatctc gatcgtgcca agaagcagtt
     4141 gatcaccagt ttccactacg gctccgaaag tatctccgag caggcgcacg gcatcggcga
     4201 tgctgcgatg tatggcaagt ccgacgactt cttcacctac ccggatgaca ttgcggcggt
     4261 cacgctcgag ggtgtcaagc aggtcgtggc ggagtatttc cggccgtcga accggaccgt
     4321 tggctacctg gtcccgaccg agcccgccag cggtggaccg gcggtcgacg gagtggccgc
     4381 cttgcggttc ggtctcggag gcgtcggcgc gccggc
//
LOCUS       DATAOZ010000044         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2126102, whole genome shotgun sequence.
ACCESSION   DATAOZ010000044 DATAOZ010000000
VERSION     DATAOZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2126102"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1482)
                     /locus_tag="VHR64_01280"
     CDS             complement(301..1482)
                     /locus_tag="VHR64_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX3301485.1"
                     /translation="MKRAPRMLLFPRTVVVMLLGAAVLGVSTPATAGTLGCWFDGTFY
                     SATIQGAGVISGTAESDVIRGSNGPDRISGYGGNDIICGRGGDDIIDAGAGADRITGD
                     SGRDTIKGAEGDDILLGGTMADRIDGGPGVNWIEGDAGDDVLIGGAGGDTIFGGEGND
                     RIYGKESSDYIEGNEGADTISGGSGGDMILGDHNGAWLTGGPDVVDAGAGNDDVWSSA
                     GADQINGGSGDDDLHGGFGDDVIAGSSGIDEIWGGPGNDLIDGGYDSDWIDGEDGADQ
                     IRGGPTGDDTILGSAGADRLYGESGNDVLKGGDDNDVIDGGDGSDQVFGGSGTDKLVG
                     GAGDDLVAGEEGDADRSYGMAGDDQVTGGPGDADYCSGGAGINSLSADHGCEILSAVG
                     A"
     gene            1640..2440
                     /locus_tag="VHR64_01285"
     CDS             1640..2440
                     /locus_tag="VHR64_01285"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX3301486.1"
                     /translation="MLDLHLHILANVDDGPGDRDESEAMLRLASSIGFTHLVATPHLH
                     EALASPYKDRVAEEQSWLSSIAEEVGITLKNGFEIRLSPDLGKRLESGEPVTLGGSRT
                     VLVELPFAGWPTFTDQALFDVMTSGFRPLLAHPERYAAALDNPNLIYALHDRGVMMQL
                     TTGSLAGLFGKRSREVAEQFLRDGIVEILASDAHSAGRRFVSVAEGIARAKELVGSER
                     MRQLTVENPRALLADQPLPAPALGYERTIGEPKWRQSLTRVKHLIPRR"
     gene            2487..2561
                     /locus_tag="VHR64_01290"
     tRNA            2487..2561
                     /locus_tag="VHR64_01290"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2519..2521,aa:Asn,seq:gtt)
BASE COUNT          482 a          922 c          773 g          536 t
ORIGIN      
        1 ccgtgttcga gaccacctgg cggtccacat ccaccacggg cggcaggatc gttgccgact
       61 tgccattcac ggacattggc gtttcctcca gtcgcgtccc actcggcgca ccgtttgacc
      121 cgagtgggtt tcgcaaaaac ctaaccaaca caaattggga gggcagattc tcccctccct
      181 aaatcggact gtgatcaatt cgtggtgtat gctcgtatgg tagtgtcacc ccgcgacgca
      241 cccgccgcta ccgccgcgaa gagcggcagt cgactagcat acctctttcc gcacatttcg
      301 tcaagcgcca actgcgctca aaatctcgca gccgtgatcg gctgacaagc tgttgatccc
      361 cgccccgccc gagcagtaat cggcgtctcc cggaccaccg gtaacttggt catcgccagc
      421 catgccatag gatcgatcgg cgtcgccctc ctcgccggca accaggtcat cgcccgcacc
      481 gccaaccagc ttatcggttc cacttccgcc gaagacctgg tcggacccat ctccgccgtc
      541 gatgacgtcg ttatcgtccc caccctttag cacgtcgttt ccggactcgc cgtagaggcg
      601 gtcggctccc gcgctgccaa ggatcgtgtc gtcgccggtc ggaccgccgc ggatctggtc
      661 agcgccgtct tcgccatcga tccaatcgct gtcgtaccca ccgtcgatca ggtcgttgcc
      721 cgggccgccc cagatctcgt cgattccgga gctgccggcg atgacatcgt ctccgaatcc
      781 gccgtgcagg tcatcgtcac cactgccgcc attgatctgg tcggcgccag cactactcca
      841 gacatcgtca ttgccggcgc cggcatcgac cacgtccggt ccaccggtca gccaggcgcc
      901 gttgtgatcc cccaggatca tgtcgccgcc cgacccgccg gaaatagtgt cggcaccttc
      961 gttcccctcg atgtagtccg agctctcctt gccgtagatg cgatcgttgc cttcgccgcc
     1021 gaagatcgtg tctccgccgg ccccgccgat caggacatcg tccccggcat ctccttcaat
     1081 ccagttcacg ccgggaccgc cgtcgatcct atccgccatc gtgccgccga gaagtatgtc
     1141 gtcgccttca gcgcccttga tcgtgtcccg accagagtcg cccgttatac gatccgctcc
     1201 agcgccggcg tcgatgatat cgtcacctcc gcggccgcaa atgatgtcgt tgccgccata
     1261 acccgagatc cggtccggac cgttcgagcc acggataaca tcggactccg cagttccgga
     1321 aataacgccc gctccctgaa ttgtggcgct gtaaaaggta ccgtcgaacc agcagccgag
     1381 cgttccggcg gtagcgggag tcgatacgcc gaggacggcc gcaccaagaa gcatcacgac
     1441 aacggttcgc gggaacagga gcatgcgggg agccctcttc acgacgtcgc ttttcacctt
     1501 tcgtcacatt gtatcaacta ttggtccggc tgatggccaa ctttcgtacc cgtcctccac
     1561 gaccggtcaa gagagccggc tggctcgtac aataggtgta tgactaatct gcgccccttc
     1621 aaactccgac ctgcccaccg tgcttgacct ccaccttcac attctggcca atgtagatga
     1681 cggacccggg gaccgggacg agagcgaagc gatgctccgg ctcgcttctt cgatcgggtt
     1741 cacgcatctc gtcgcaacac ctcatctgca tgaggcgctc gcatccccgt acaaggatcg
     1801 ggtcgcggag gagcagagtt ggctttcctc tatcgctgaa gaggtcggga tcacgctcaa
     1861 gaacgggttc gagattcggc tctctccgga tctcggcaag cgcctggagt caggtgagcc
     1921 ggtgacgctt ggggggtccc gaactgtgct ggtcgagctc ccatttgcgg gttggccgac
     1981 cttcactgac caggcgctgt tcgacgtcat gacctccgga ttccgaccgc tgctcgccca
     2041 tcccgagcga tatgccgccg cgctcgacaa ccccaacctg atctatgcgc tgcacgatcg
     2101 gggcgtgatg atgcagctca cgaccgggag cctcgccggc ctgttcggga agcggtcccg
     2161 ggaagtcgcc gagcaatttc tgcgcgacgg gatcgtcgag atcctggctt cggacgcaca
     2221 ttcggccggt cgccgtttcg tctcggtcgc cgaagggatc gcgcgtgcca aagaactcgt
     2281 gggatcggag cggatgcggc aattgactgt cgagaatccg cgggcgctcc tcgccgacca
     2341 gccacttccg gcaccggccc tcggatacga gcgaacgatt ggcgagccga agtggcggca
     2401 gtcgctgacc agggtcaagc acctgattcc gagacggtga cgacccgccc cgcgtttggt
     2461 ataatcacgg accgactgag gcctattcct cgatagctca acggtagagc ggccggctgt
     2521 taaccggtag gttccaggtt cgaatcctgg tcgaggagcc acgtcagagc gccgcgggga
     2581 cagacccggg cgcttttttg ttgactttgc gcgccacctt accaaggcat gcaggacatg
     2641 caagcagcga acgttgtcca cttctaccag cagtgcgccg gttcgggaat cgctgtttgg
     2701 gttgatggcg gct
//
LOCUS       DATAOZ010000045         5859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8965279, whole genome shotgun sequence.
ACCESSION   DATAOZ010000045 DATAOZ010000000
VERSION     DATAOZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8965279"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VHR64_01295"
     CDS             complement(<1..1061)
                     /locus_tag="VHR64_01295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3301487.1"
                     /translation="MTRAYNVIDADGHILEPVDLWDRYIDPKFRECRPRFVIDDNGKE
                     RLSVEGKLLGNPRGIGSLGSVGARQGIVKSGTLKYAEGRKGGFDPHARIVDMDADGID
                     AAFLYPSLGLFAGAVEDPELAAAMCRAYNRWLVDYCAPYPERLFGVAMLPMQSVELAV
                     DEMRFAREKLGIRGGFLRPNPYHGKKMISDPMYEPFWATAEDLDFSIGFHEGSTNAMP
                     TVGVDRFEDDRAARHMVSHTMEIMLVALSVIWGGVCERHPKIRIGFLESGGGWIAPWL
                     DRMDRHFDDQFFNDSNLKTRPSEIFQRNCWISFEPVETSLQVLADFIGPNKILWATDY
                     PHPDGFFPGAPRMVRDLIK"
     gene            complement(1105..2298)
                     /locus_tag="VHR64_01300"
     CDS             complement(1105..2298)
                     /locus_tag="VHR64_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3301488.1"
                     /translation="MGTALEGIRVLDLSFYEAGPSCAESLAWLGAEVIKIEAPTGEPA
                     RYGFTDKPGVDSVFFCLMNLNKKSVTLNLKSPQGREMFQALAKTADVVIENLGPGAME
                     RLGLGHDALSKINPRIITASVKGFGSEGPYASYKSFEMIAQAMGGVMSLTGEPDGPPM
                     RIESGIGDTATGLHAAIGILGAIIQRGITGVGQRVEVAQQDVVVNLTRIHFRDHWLGV
                     SPVPRKGNRSPNAAPSNLYRCRPFGPNDYVFIHVANPEMWKGLISVITHPELADPRLA
                     DRAERMKRRDELEAIVEGWTSARDKHEVMALLGAAGVPCGAVLDSGEVMANEHLRGRG
                     MIVDIEHPTRGRMATPGNPIRLSASSTEVTRAPLLGEHNAEILGKVCGLGTEELAKLK
                     ADGVI"
     gene            complement(2374..3216)
                     /locus_tag="VHR64_01305"
     CDS             complement(2374..3216)
                     /locus_tag="VHR64_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3301489.1"
                     /translation="MAIIDSQVHVYEANTPKRPWHSVPNWPDHVTGDEMVAAMDTVGV
                     DGAIFISAFSMYRYDASYAVEVQRAHPGRFALVKPVDPDDPAVADVIADWKKTPGTVG
                     IRIMMTKEAGRDPRDPGLDRILRAAVRYDFPVNMLCWGNVDAGAALIDRHPDTRFIID
                     HLALMQPRTPPAPPQPWADLPKVLDLARRPNAVIKVSGACTLSREPYPFPDIWDPLAR
                     VFDAWGFERCLWGTDWTRAFAVVNYEQAVEPFRQTKRLSDSERAMLMGGACAKAYGWS
                     PKKG"
     gene            complement(3871..5013)
                     /locus_tag="VHR64_01310"
     CDS             complement(3871..5013)
                     /locus_tag="VHR64_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3301490.1"
                     /translation="MTSRALLAVVTVFLAACTVAPKPDGNGSAMLAPDSMESVVKRTF
                     TTYGFRLQSGKTLPEMTLAFETYGRLAPDGRNAILVTHGGTGGQHMAGKYSPTDRAPG
                     GWDTLIGAGKAIDTNKYFVVSSNVLGSSYGSTAPASINPATGKRYGPDFPEISMHDIV
                     TAQRTLLRGLGVHRLVAVVGPSYGGYQAFQWSVSYPDDMAGIAAVITAPRGSGKESDV
                     QALVDRFAKDPNWNGGWHYDRGGIPDTLTTLRIETLTRYGWNEILAEKFPTQAERDAE
                     IRRRAEPWAKEFDPNSMVTMRRAAVRYNAEKDFSKIRAKVLYVISRSDKLFPPSIAPD
                     VMDKLAKAGVDAKYFEIDTDFGHSATGVEWAKWGPTLKAFVEGLSK"
     gene            5353..5502
                     /locus_tag="VHR64_01315"
     CDS             5353..5502
                     /locus_tag="VHR64_01315"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEX3301491.1"
                     /translation="MDFFRELGAFMLERKKFWLLPIVGILGIFGGLIVATEGMAIAPF
                     IYSLW"
BASE COUNT          958 a         1974 c         1909 g         1018 t
ORIGIN      
        1 tccttgatca ggtcacgcac catccgcggg gcacccggga agaagccgtc gggatgtggg
       61 tagtcggtcg cccacaggat cttgttcggg ccgatgaagt ccgcaagcac ctggaggctg
      121 gtctcgacag gctcgaacga aatccagcag ttgcgctgga agatctcgct cggccgcgtc
      181 ttcaggttgg aatcgttgaa aaactgatcg tcgaaatggc ggtccatgcg gtcgagccag
      241 ggcgcgatcc agccaccgcc cgactccaag aacccgatcc ggatcttcgg gtgccgctcg
      301 cacacaccgc cccagatcac gctcagcgcc accagcatta tctccatggt gtgggagacc
      361 atgtgacgcg ccgcccggtc gtcctcgaag cggtcgacgc cgacggtcgg catcgcgttg
      421 gtcgagcctt cgtgaaagcc gatcgagaag tcgaggtcct cggccgtcgc ccagaacggc
      481 tcgtacatcg gatcgctgat catcttcttg ccgtgataag gattgggccg caggaacccg
      541 ccgcgtatcc cgagcttctc gcgggcgaag cgcatctcgt cgactgccag ctcgaccgac
      601 tgcatcggca gcatcgcaac cccgaacaga cgctcgggat acggcgcgca gtagtcgacc
      661 agccagcggt tgtaggcgcg gcacatcgcg gcggccagct ccggatcctc cacggcaccg
      721 gcgaacaggc cgagactggg atagaggaag gcggcatcga tgccgtcggc atccatgtcg
      781 acgatgcgcg cgtgcggatc gaacccgccc ttgcgcccct cggcgtactt cagagtgccg
      841 gacttgacga tgccctgtcg ggcgccaaca gaccccaggc tgccgatgcc ccgcgggttg
      901 cccagcagct tgccctcgac gctgaggcgc tccttgccgt tgtcgtcgat gacgaaccgc
      961 ggccggcact cgcgaaactt cggatcgatg taccggtccc agagatcgac tggctcgagg
     1021 atgtggccgt ccgcgtcgat cacgttgtac gcgcgtgtca tcggcaatcc ctccgaaggc
     1081 aatcggaccg ctccgatact actgttagat caccccatcc gccttcagct tcgccagctc
     1141 ctccgttccg agcccgcaca ccttgccgag aatctctgcg ttgtgctcgc ccagcagcgg
     1201 tgcgcgcgtc acctcggtcg atgacgccga gagccggatc gggttgcccg gggtcgccat
     1261 gcgcccgcgt gtgggatgct cgatgtcgac gatcatgccg cggccccgca gatgctcgtt
     1321 cgccatcacc tcgccggagt cgaggacagc gccgcacggg acgccagccg cgccgagcag
     1381 cgccatcacc tcgtgcttgt cgcgggcgga cgtccagccc tcgacgatcg cctccagctc
     1441 gtcgcgccgc ttcatgcgct cggcacggtc tgcaaggcgc gggtcggcga gctccggatg
     1501 tgtgatgacg ctgatcagcc ccttccacat ctccggattc gcgacgtgga tgaagacgta
     1561 gtcgttcgga ccgaacggac ggcagcgata gaggttcgac ggcgcggcat tcggggatcg
     1621 gttgcccttg cgcggaacgg gggagacgcc gagccagtgg tcgcggaaat gaatgcgggt
     1681 gagattcacc acgacgtcct gctgcgcgac ttcgacacgc tgcccgacgc ccgtgatgcc
     1741 gcgctgaatg atcgccccca ggatgccgat cgccgcgtgg aggccggtcg ccgtatcacc
     1801 gatgcccgac tcgatccgca tcggcggccc atccggctcg ccggtcaagc tcatcacacc
     1861 gcccatcgcc tgggcgatca tctcgaagct cttgtaactg gcgtacggcc cctcgctgcc
     1921 gaatcctttg acggacgcgg tgatgatgcg cgggttgatc ttcgagagcg cgtcgtgccc
     1981 gagcccgagg cgctccatcg cgcccgggcc gaggttctcg atcaccacgt cggcagtctt
     2041 cgccagcgcc tggaacatct cccgcccctg cggcgacttg aggttgagcg tcacgctctt
     2101 cttgttcagg ttcatgaggc agaagaagac cgagtccacg cccggcttgt cagtgaaccc
     2161 gtaacgcgca ggctcgcccg tcggggcctc gatcttgatg acctcggccc ccagccacgc
     2221 cagcgactca gcgcagctcg ggcccgcctc gtaaaacgaa agatcgagaa cccggattcc
     2281 ctcgagcgct gtgcccatcg ttcgcctcct gtgtgatctg cgcgttgccc tcaccacgga
     2341 tcgatgcaag ggtaccggaa tcgagtgtgt gcgctaccct ttcttcggag accagccata
     2401 ggctttggcg cacgcaccgc ccatcagcat cgcccgttcg ctgtcgctca ggcgcttggt
     2461 ctggcggaag ggctcgacgg cctgctcgta gttgacgacc gcaaaggcac gcgtccagtc
     2521 ggtgccccac aggcagcgtt cgaaacccca ggcatcgaac acgcgggcga gcgggtccca
     2581 gatgtccggg aaaggatagg gttcccggga cagcgtgcaa gcaccgctga ccttgatcac
     2641 cgcgttcggg cgcctggcga ggtcgagcac cttcggcagg tcggcccagg gctgcggggg
     2701 ggcgggcggc gtccggggct gcatgagggc aaggtggtcg atgatgaatc gcgtgtcggg
     2761 atgccggtcg atcagcgccg cgcccgcgtc gacgttgccc cagcacagca tgttgactgg
     2821 aaaatcgtac cgaacggccg cgcgcaggat ccggtcgagg cccgggtcgc gcggatcgcg
     2881 ccccgcttcc ttcgtcatca tgatccggat gccgaccgtg cccggcgtct tcttccagtc
     2941 ggcgatgacg tcggccaccg ccggatcatc gggatcgacg ggcttcacga gcgcgaaccg
     3001 gccgggatga gcccgttgca cctccacggc gtagctcgcg tcgtagcgat acatggaaaa
     3061 ggcggagatg aagatcgcgc cgtcgacacc gaccgtgtcc atggcggcca ccatctcgtc
     3121 gccggtgacg tgatcaggcc agttcgggac actgtgccag ggccgtttcg gggtgtttgc
     3181 ctcgtagacg tggacctggg agtcgatgat cgccatcggg aggttcctcc gttccaagtg
     3241 gttcatcgta tataccgcga agtgacgtgg cctgccgtcc cttctgacca ccggcggtga
     3301 gcacgatatc ggcgctggca ttcggtcgtg tcgaccgctc ggcaagcggc ggtaagggca
     3361 attgaggcct ggcctggagt gggccgagac gtcgcccaga gacgcctctc aggcagtgga
     3421 cttgaaatca gagccagcgt ctagctctcc gtagcactcc agcgcactcg cgcaccactt
     3481 ccggcaatgt tcacgccact gcccgcgtcc gggtgagtgc gcgctcaggc cgctacgtgg
     3541 ccgcaggccg gggcctcatg agcttcggac cggatgttgc ggatctgtac gaacgtgccg
     3601 cgattcacgt ggcaaagacc ttgaggggtg cgaacgcggg tgatcttccg gtcgagcagc
     3661 ccagcgagtt cgagctggcg atcaacctca agaccgccaa agccctcggc ctcacagtca
     3721 gtcgcagcct cggccgaacg gccattgctg cggccgattc gccgtcggcc ccgctgcgcc
     3781 gcgcgacggc cggcacacgg agaggcgcgc gcgaccgtga ctctcgatcg ccactacgcg
     3841 gcacggtctt ccagccggac tgcgttcgcg ttacttcgac aaaccctcca cgaacgcctt
     3901 cagcgtcgga ccccacttcg cccactccac tccggtcgcg gaatggccga agtccgtatc
     3961 gatctcgaag tacttcgcgt cgacgcccgc cttcgcgagc ttgtccatca catcaggcgc
     4021 gatcgacggc ggaaacagct tgtccgagcg cgagatcacg tagagcactt tcgccctgat
     4081 cttcgagaaa tccttctcgg cgttgtaccg gacggcggcg cgacgcatcg tgaccatcga
     4141 gttcggatcg aactccttcg cccacggctc ggcgcgccgg cgaatctccg cgtcgcgctc
     4201 ggcctgcgtc ggaaacttct cggcgaggat ctcgttccac ccgtagcgcg tcagcgtctc
     4261 gatgcgcagc gtcgtcagcg tgtcgggaat cccgccgcgg tcgtaatgcc agccgccgtt
     4321 ccagttcggg tctttcgcaa accggtcgac gagcgcctgg acatccgatt ccttgccgct
     4381 gcccctcggc gccgtgatca cggccgcgat gccagccata tcatccggat acgacaccga
     4441 ccactggaag gcctggtagc cgccgtacga cgggccgacg accgcgacga gacggtgcac
     4501 gccgagcccg cgcaacagcg ttcgctgcgc cgtcacgatg tcgtgcatcg agatctcggg
     4561 gaagtccggc ccgtagcgct tgcccgtcgc cggattgatg ctggccggcg cggtcgaccc
     4621 atacgacgac ccgagcacgt tgctggagac cacgaagtac ttgttcgtgt cgatcgcctt
     4681 gccggcgccg atcagcgtgt cccatcctcc cggtgcgcgg tcggtcggcg agtacttccc
     4741 ggccatgtgc tggccgcccg tgccgccgtg cgtgacgaga atcgcgttgc gaccatccgg
     4801 cgccagccgt ccataggtct cgaacgcgag cgtcatctcc ggcagcgtct tgccgctctg
     4861 caggcggaag ccgtaggtcg tgaacgtgcg cttcacgacc gactccatcg agtcgggcgc
     4921 cagcatggcg ctgccgttgc cgtcgggttt cggcgcgacg gtgcaggcgg cgaggaacac
     4981 ggtcacgacg gcgagcaggg cacgagacgt catgggtgcc tcctcatcgc cggagtttct
     5041 cccagaacgg agcgttggtc cagaggcatg ggcccgcccc caacacgccc agcgtacaga
     5101 tctcggcgat cgccgcgtcc gggtgccctc aggctcccgg cgcgcgggag cggactcatc
     5161 cgacgcgacc cgggaaggaa tgctgttgcc cgatgtctgg ataagtggcg ggcaattgga
     5221 gcctagccag tggtgagccc ggacagctgc gtggtctgct cgaccgccat gggcggagca
     5281 gaggactgcc gaggcccagc tcaccgatgg gtccaggacc cgagggctca atcttgactg
     5341 gagggtgaca cagtggattt cttccgggaa ctcggcgcct tcatgctcga gcgcaagaag
     5401 ttctggctgt tgccgatcgt ggggatcctc gggatcttcg gtggcctgat cgtcgccacc
     5461 gagggcatgg cgatcgcgcc gttcatctac agcctctggt agcgcctcac gaccgccgca
     5521 gtcggcgcgg cggccgcgga gcactgcggt cgctgctccg cccacgctga agctggctcc
     5581 gggcaaggcc ccagccaatt ggggtagtct cggccttccg ccctccgggg gagaaatggc
     5641 ccaggcggct tctgcggggt cgcccggcgc ctattggcct tccaccgggg ctcgagaagg
     5701 aatgttgcgg cccagtgtct tgatgagtgg aggtatgcgc gtggggcgcg cccggtgatg
     5761 cccgaagatc tgcggtgtct gctcgatcgt tgccgggcgg aggagagccc tgcctgggag
     5821 ctgttcaccc agtgggtgag aacccgagcc gcggcgatc
//
LOCUS       DATAOZ010000046         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6335145, whole genome shotgun sequence.
ACCESSION   DATAOZ010000046 DATAOZ010000000
VERSION     DATAOZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6335145"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..565
                     /locus_tag="VHR64_01320"
     CDS             254..565
                     /locus_tag="VHR64_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3301492.1"
                     /translation="MTSDNISRLAPAARRLRGPDRLIVGWLARQLASYLELPETAIDP
                     TVPLAEMGVDSVHAVSLVGDVEMHFDIDVDPTLIFDYPTLSHIAEYISAAVAEQQEVV
                     A"
     gene            566..1450
                     /locus_tag="VHR64_01325"
     CDS             566..1450
                     /locus_tag="VHR64_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3301493.1"
                     /translation="MVVVTGVDFDRHEVRRSRPPVKAAVAHRRMITLNGHRIAYRDAG
                     AGEVLLLVHGLGGSSSSWAEVIPKLSGKYRVIAPDLLGHGRSDKPRGEYSPAVFAGLL
                     RDLLDALGIDKATVVGHSLGGGVAMTFASQHRNYCRRLVLLNSGGFGSEVSVLLRMLS
                     VRGTGFVLPLIAAVRAVWTGGASTVREVVPSRDDRRVFLRTLRSVVNLRGQAASALAC
                     LHFFADLPTQIIGGENDQVIPVAHAHAAHTALPGSRLDIIPGAGHDPQMQRPEIVAGL
                     IDDFVAQAAEVSSLALAA"
     gene            complement(1466..>1772)
                     /locus_tag="VHR64_01330"
     CDS             complement(1466..>1772)
                     /locus_tag="VHR64_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX3301494.1"
                     /translation="TDGTMLAVREDIGRHNAVDKVVGWALEANRIPLTATVLLVSGRA
                     SFELTQKAVMAGIPVLAAVSAPSSLAVDLASQSGLTLVAFLRGDSMNVYTRPDRIKR"
BASE COUNT          283 a          556 c          595 g          338 t
ORIGIN      
        1 gaccagcccc tgagcgatgt tgagcagggg accgacgtgg ccgagcgggg agagcgaggc
       61 gatgaggact tcaggcatgg gatcgatgct cgatcagtgc ggcggcgcaa gcttggaggt
      121 tgtgtggaga tttcctggag attgcgagtg acgcggcact tgccgggcag ctagaaaacc
      181 ctatccgttc acgagcggaa tctggcttcc cggcgattac ggcaccaatg cctgatcaaa
      241 gaccatttca tgcatgactt cagacaacat ttcccgcctc gctccggccg cccgccggct
      301 caggggacct gaccgcctga tcgtgggttg gctcgcgcgc cagctcgctt cctacttgga
      361 gctcccggag accgcgatcg atccgacggt tcctctcgcc gagatgggcg tcgattcggt
      421 acatgcggtc agcctcgtcg gcgacgtcga gatgcacttc gacatcgacg tcgatccgac
      481 cttgatcttc gactacccga ccctctcgca catcgccgaa tacatcagcg ccgctgtcgc
      541 ggagcagcaa gaggtggtgg cgtaaatggt cgtcgtcaca ggcgtcgact tcgatcgcca
      601 cgaggttcgg cgatcgcggc cgcctgtgaa agctgcggtg gcacaccgca ggatgatcac
      661 cctgaatggt caccggatcg catatcgcga cgccggagcc ggcgaggtgt tgctgctggt
      721 ccacggcctg ggcggtagtt cgtccagctg ggcggaggtg attccgaaac tgtcgggcaa
      781 gtatcgcgtc attgcaccgg atctattggg ccatggccga tcggacaagc cgcgcggcga
      841 gtattcgccg gccgtgttcg caggcctgct tcgtgatctg ctcgatgcgc ttgggatcga
      901 caaggcgacg gtggtcggcc actctctcgg cggcggggtg gcgatgacgt tcgcctcaca
      961 gcaccggaac tactgcaggc ggctggtcct gctcaatagt ggcggcttcg gctcggaggt
     1021 cagtgttctg ctgcggatgt tgtccgtgcg cggcactgga ttcgtattgc cgctcatcgc
     1081 cgcggtgcgc gccgtgtgga ccggcggggc ctcgaccgtc cgtgaggtgg ttccgagtcg
     1141 ggacgaccgg cgggtgttcc ttcggactct gcgatcggtg gtcaacctgc gcggccaggc
     1201 ggcctcggca ctggcatgcc ttcacttctt cgcggatctg cctacgcaga tcatcggtgg
     1261 tgagaacgat caggtcatcc cggtcgccca cgctcatgcg gcgcacaccg cgctgccggg
     1321 cagtcgactc gacatcatcc ccggtgccgg acacgatccg cagatgcagc gacccgagat
     1381 cgtggccggt cttatcgacg actttgttgc ccaagccgcc gaggtctcgt cgttggccct
     1441 ggcggcctag ccggccgagt cgcgctcagc gtttgatgcg gtctggccgg gtgtagacgt
     1501 tcatcgaatc gccgcgcagg aacgcgacga gcgtgagtcc ggactggctc gcgaggtcca
     1561 ccgccagcga cgacggcgcc gaaaccgcgg caagcaccgg aatgcctgcc atcacggcct
     1621 tctgagtgag ttcgaatgac gcccgcccac tgaccaggag cacggttgcc gtcagcggga
     1681 tgcggttggc ttccagcgcc cagccgacga ccttgtcgac cgcgttgtgc ctgccgatgt
     1741 cttcgcggac ggccagcatg gtgccgtcgg tg
//
LOCUS       DATAOZ010000047        12985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6598681, whole genome shotgun sequence.
ACCESSION   DATAOZ010000047 DATAOZ010000000
VERSION     DATAOZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12985
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6598681"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2450)
                     /locus_tag="VHR64_01335"
     CDS             complement(<1..2450)
                     /locus_tag="VHR64_01335"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX3301495.1"
                     /translation="MTGRDQAVQLAPAFNPKEVEQGLYDQWDAAGYFQPSDRTDREPF
                     VVIMPPPNVTGELHIGHALFVTIQDIMVRYHRMLGDPTLWLPGADHAGIAGQWVVERL
                     IAKEGLTRHDLGRERFLERVWEYMDQYRGRIREQMRILGASCDWSRFTFTMDPGPSRA
                     VRRTFKHLYDKGMIYRGERLISWCPRCNTALSDLEVIHRDVKGHLWHIAYPVEGSDER
                     VVVATTRPETMLGDTAVAVHPDDERYQHLIGKMIRLPIMDRLIPIVADDAVDSSFGSG
                     AVKVTPAHDPNDFEIGRRHDLPFVTVMNLDGTMNAEAGPFEGLTIDDARVAVVKRLEQ
                     DGFLVKTENHEHSVGHCERCGTVVQPLISKQWFVRMDELAAPAIAAAKDGSVQFVPER
                     FKGVYLNWMENIRDWTVSRQLWWGHRIPVWYRLSDGQPIVSEVDIDVDPETGEPVEQD
                     PDVLDTWFSSGLWPFSTLGWPDETEDLKRFYPSSVMETGYEILFFWVARMVFFGIEMM
                     GEPPFHTVYLHGTVRDAEGLKMSKTKGNVLDPTEITEEYGADALRFALATQGSAGIDS
                     RLSLGQVESSRNFINKLWNATRFAMGAIQRGSIEMTTDGPARPTANLSIVDRWILSRT
                     DAVTAEMTRLLDTHQYGEAGRQLREFIWSEFCDWYIEAAKVRLRGEQQDVETVAQTLA
                     YAINRILRLLHPYAPFVTATLWQVVPNAGAALIVSDWPRGGESNPEAERLMGTVIELV
                     TKVRNARSESNVEPGRWIAAHISSPADSAALESLRGEISFLARIANDQLTFSDTTPAA
                     HKSDIVIAVGDVVAVLPMA"
     gene            2622..5528
                     /gene="uvrA"
                     /locus_tag="VHR64_01340"
     CDS             2622..5528
                     /gene="uvrA"
                     /locus_tag="VHR64_01340"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX3301496.1"
                     /translation="MPQNDRIVVRGAREHNLKNIDVEIPRDKLVVFTGLSGSGKSSLA
                     FDTIFAEGQRRYVESLSAYARQFLGQMEKPDIDHIDGLSPAISIDQKGTSRNPRSTVG
                     TVTEIYDYLRLLYARVGHPHCPNCGRPISHQTVQQMAGTILGLPDGTRLMILAPVIED
                     RKGEYLAVFDEIRRQGFVRVRVDGEVRDLDETITLEKNKKHTIEVVVDRVIVRHDEGS
                     ELDDNPDRVRIIDSLETALRIGNGQAVGQIIDGEALRFSEKFACVHCGLSFGELEPRN
                     FSFNSPHGACPTCTGLGTRLEFAPELIVPNRNLSLAQGAIMPWMRAGGDNSTWYYALL
                     NAMCEKYGQSMDVPYSELPKDFVDLLMNGNKQKVTVRYKSGSGRWRSYDVPFEGVIPN
                     LQRRLQNTTSDYVLEELGRYQAARLCPTCKGARLKPEARAVLVDGKGIVEITTMSCRD
                     SREFFDRLSADPADVPGAPLTEREYLIARQILKEIRERLGFLVDVGLDYLTLDRSATT
                     LSGGEAQRIRLATQIGSSLMGVLYILDEPSIGLHQRDNERLIRTLERLRDIGNTLIVV
                     EHDEDMMRAADWIIDIGPGAGVHGGHVVAAGTMADIARSPESITGQYLTGERRIEVPM
                     MRRPGNGKFLELRGASENNLQDVDVKIPLGVFVAVTGVSGSGQSSLVSDTLHKRLAID
                     LNGARERAGAHDALFGVEHLDKVIDIDQSPIGRTPRSNPATYTGVFTPIRELYASMPE
                     AKARGYKPGRFSFNVKGGRCEACSGEGLIKIEMNFLPDVYVPCEVCHGKRYNREALEI
                     HYKGKSIADVLAMTVSEGVQFFENIPAIRNKLQTLQDVGLGYITLGQPATQLSGGEAQ
                     RVKLASELSRRATGKTIYILDEPTTGLHFADISKLLDVLQRLTDGGNSVMVIEHNLDV
                     IKCADWIIDLGPEGGNGGGRIIAEGTPEAVAANPESFTGEYLRHVLEPAAVAAD"
     gene            5657..6202
                     /locus_tag="VHR64_01345"
     CDS             5657..6202
                     /locus_tag="VHR64_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX3301497.1"
                     /translation="MSGTVLVLNQNYEPLNICNVRRAIVLVIDGKAEVLEQYSFALHA
                     ASREFPFPSVIRLVYMIKRPRPRVKLTRREIFIRDDYTCQYCGKHTSDLTIDHIHPRS
                     KGGPHTWDNLVSACRSCNHRKGGKSLAEARMQLKRQPFEPKPGAYYTIQRRVESGIHP
                     DWEKFLPGYQVLIEPARAFSS"
     gene            6294..7568
                     /gene="serS"
                     /locus_tag="VHR64_01350"
     CDS             6294..7568
                     /gene="serS"
                     /locus_tag="VHR64_01350"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX3301498.1"
                     /translation="MIDIRIIRERPDEVKHALELLNTSAPIDRIIELDAERRRLVTDV
                     DQLKATRNEGSKAVSKTKDPDERNRLIAEMKQVSDSITAMDARITTVDQEFNDLMLQV
                     PNMPMPEVPVGKDDSENVVVRTVGEIRERDFTVRPHWETGVDLGIIDFERGVKVAGSR
                     MYVLKGLGARLQRAIITWMLDLHIKKHGYSEVYPPYLVTSDTLIGTGNLPKFGDNLFK
                     DYLEDRWMIPTAEVPVTNLYRDEILDEGQLPIYHVAYTPCFRREQLSAGRDVRGIKRG
                     YQFDKVEMVKFVHPERSSAEHEQLIQEAADVLEALDLPYRVVQMCTGDLSFTAANKLD
                     IEVWAPGSQEWLEVSSCSNFRDFQARRANLRFRPEGGGRPQYLHTLNGSGLALPRTMI
                     AIMENYQNSNGSFEIPEVLLPYMDGVTRISGA"
     gene            complement(7565..8686)
                     /gene="gcvT"
                     /locus_tag="VHR64_01355"
     CDS             complement(7565..8686)
                     /gene="gcvT"
                     /locus_tag="VHR64_01355"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX3301499.1"
                     /translation="MGRRRSPYYAKYVELGAELVDRMGYDAPYRFSTTEAEHMATRTA
                     AGLYDVYHQGMVDIKGKDAEALLQNACVNDLTRIVDGHVLYSSVCKDDGGMIDDLTIY
                     RIAPDHFFLSPTPSRVEAIVAHLTEQARGMNAYVTDRVSGTGFISVQGPNAREIVSQV
                     TDADLSTDALPYYTFTKTTLAEVPGYLARTGYSGELGFEFFYPVEYGIYMWDSVYNAG
                     KEHGLVPCGLGALRSIRMEKRYPLYGLDLDETTSPIEANLGWTVRFGKGDFNGRDALL
                     RQKEKGVDRNLVAIEFDGLDFLPATGDAVSIDGSAVGKVTSADRGFFVGKSIALAYLQ
                     PDAATTGTRVTVTDKTGAQATGMVNQRAAYDPDREKVRA"
     gene            8814..8897
                     /locus_tag="VHR64_01360"
     tRNA            8814..8897
                     /locus_tag="VHR64_01360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8848..8850,aa:Ser,seq:tga)
     gene            8986..9606
                     /locus_tag="VHR64_01365"
     CDS             8986..9606
                     /locus_tag="VHR64_01365"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX3301500.1"
                     /translation="MAVATDTRVDAYIEHAPEFARPILVKLRELVHIACPDVTETIKW
                     GVPAYEYEGILAIMASMKRHAVFNLWKGELIPEVKELYSEKFSMAMGTFGKITSVDDL
                     PPDDKIISWIHAAMDLNERGVKLPQRSRTEPRPEAEVPDDLVAALEQNEPARLTFEGF
                     PPSHRRDYVEWITEAKTGATRQKRLTQAVEWMAGGKHRNWKYDKKK"
     gene            9615..10142
                     /locus_tag="VHR64_01370"
     CDS             9615..10142
                     /locus_tag="VHR64_01370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX3301501.1"
                     /translation="MTDDITIRPERPDDVQAIHDLVREAFGRELEADVVAKLRESAGF
                     LADLSLVAVRDSQVVGHIMITELDYPSDAGGRVQSTILAPLAVLPHFQNQGIGDALSR
                     VALERTKEAGYGSMILVGHPNYYPRFGFRPASNWGIRYATPIPDDVFMAVELVPGSLD
                     DAAGTVILPSAFDEA"
     gene            10271..10361
                     /locus_tag="VHR64_01375"
     tRNA            10271..10361
                     /locus_tag="VHR64_01375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10305..10307,aa:Ser,seq:gct)
     gene            complement(10416..11396)
                     /locus_tag="VHR64_01380"
     CDS             complement(10416..11396)
                     /locus_tag="VHR64_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX3301502.1"
                     /translation="MPGPAPEIEIDATDLRRRGPSLPLGTPRSGTYRVEFTNGECYVG
                     ETHNIVLRLGSHGRTWDDMRAIRFWPGKDCEERSALADRSPVRVARPSPPLRKVDAEA
                     PEIDPVLDRMQWSDDLLLGRQDARPIERPDQRERTRPLFDRLSKHPDFERIATLVARY
                     LDLFVPAPASTEVAHWVITSLPSTAKTRLWHRLACLSINNVEALTIGEQFDGKCWTTL
                     GFMSANPSERHPDKLLTRDVQSRGVFVASAWYKTVGPVYQVGFDDLDGLEAALEDEEV
                     LDLVGAMAMRLMRRGRGLYGRFHDYNLADVVLGTPSPGENRDIQDRTAND"
     gene            11462..11534
                     /locus_tag="VHR64_01385"
     tRNA            11462..11534
                     /locus_tag="VHR64_01385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11495..11497,aa:Arg,seq:gcg)
     gene            11620..11705
                     /locus_tag="VHR64_01390"
     tRNA            11620..11705
                     /locus_tag="VHR64_01390"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11654..11656,aa:Ser,seq:cga)
     gene            11743..11832
                     /locus_tag="VHR64_01395"
     tRNA            11743..11832
                     /locus_tag="VHR64_01395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11779..11781,aa:Ser,seq:gga)
     gene            11938..12327
                     /locus_tag="VHR64_01400"
     CDS             11938..12327
                     /locus_tag="VHR64_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3301503.1"
                     /translation="MRIAFTSILVDDQDRALAFYTDMLGFLPKTDFPVGDFRWITVVS
                     PDAPDGTELVLEPNVNPAALAFQTAMHEQGIPLTAFQVDDIAGEYARLREKGVVFTQE
                     PTEAGPVTIALFDDTCGNLIQIFQLNG"
     gene            complement(12333..>12985)
                     /locus_tag="VHR64_01405"
     CDS             complement(12333..>12985)
                     /locus_tag="VHR64_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3301504.1"
                     /translation="DAPSGVTLITVEDSSGQNRIAYVPGATMTVTPEEARSAVDTLTP
                     RVLLTTLELPAESIAAAIEAVRIRHGLVLLNATPEPEGAARFLGDIDCLIVNEPEAMA
                     LLHRQAPADWFAAAEMLREQGPRWVIITIGPEGAVASFEGERVAIPAPAVRVTDTTGA
                     GDALCGAFAAALAAGSTPNEAIRRGVAAGSVACTIDGAQRSMPTTKQIDDLLAAVK"
BASE COUNT         2485 a         4079 c         4031 g         2390 t
ORIGIN      
        1 gccatcggca gcacggcgac cacgtcgccg acagcgatca cgatgtcgga tttgtgcgcc
       61 gcgggcgttg tgtcactgaa agtcaattga tcgttggcga tccgggccag gaaactgatt
      121 tcgccgcgca gcgattccag cgctgccgaa tccgccggcg atgagatgtg cgccgcgatc
      181 caccggcctg gctcgacgtt ggattcgctc cgcgcatttc gaaccttcgt cacaagctcg
      241 ataaccgtcc ccatcagccg ctcggcctcg ggattcgact cgccgccccg cggccaatcg
      301 gacacgatca acgccgcgcc ggcattcggg acaacctgcc agagtgtagc cgtcacgaac
      361 ggtgcgtagg gatggagcag cctcaggatg cggttgatag cgtaggcaag cgtctgcgcc
      421 accgtttcga cgtcctgctg ctcgccacgc agccggacct tggccgcctc gatgtaccag
      481 tcgcagaatt ccgaccagat gaactcccgc agctgcctcc cggcctcgcc gtactggtgc
      541 gtgtcgagca gccgggtcat ctcagccgta acggcatcgg ttcggctcag gatccaccgg
      601 tcgacgatcg agaggttcgc cgtcggccgg gccggaccgt ccgtagtcat ctcgatcgat
      661 ccccgctgga tggcgcccat ggcgaaccgg gtcgcgttcc agagcttgtt gatgaagttg
      721 cggctggatt cgacctggcc gaggctgagg cgcgaatcga taccggcaga gccctgcgtc
      781 gcgagcgcga atcgcagggc atcggcaccg tactcttccg tgatctcggt cggatcgagc
      841 acgttacctt tggtcttgct catcttgagc ccctcggcgt cgcgcactgt gccgtggagg
      901 tagaccgtgt ggaagggagg ctcacccatc atctctatgc cgaagaagac catgcgggcg
      961 acccagaaga agaggatctc gtagccggtc tccatgaccg acgacgggta gaagcgcttg
     1021 aggtcctcag tctcgtcggg ccagccgagc gtcgaaaacg gccacaggcc cgagctgaac
     1081 caggtatcga gcacgtccgg atcctgctcg acaggctcgc cggtctctgg atcgacgtcg
     1141 atatcgactt cgctgacgat tggttgtcca tcactcaagc gataccaaac cggaatccgg
     1201 tgaccccacc acaactggcg tgagacggtc cagtcgcgga tgttctccat ccagttcagg
     1261 tagacgccct tgaagcgctc cgggacgaac tgaaccgagc catccttggc cgcggcgatg
     1321 gccggcgctg ccagctcatc catgcggacg aaccactgct tgctgatcaa aggctgaacg
     1381 accgtaccgc agcgctcgca gtgaccaaca gagtgctcat ggttctcggt cttgacgagg
     1441 aagccgtcct gctcgagtcg cttcacgacc gctacgcggg catcgtcgat ggtcaagccc
     1501 tcgaatgggc cagcctcggc gttcatcgtg ccatccaggt tcatgacggt cacgaaaggc
     1561 aggtcatggc ggcggccgat ctcgaagtcg ttggggtcgt gggccggagt caccttgacc
     1621 gctcccgagc cgaacgagga atcgaccgcg tcgtcggcaa cgatcgggat caagcggtcc
     1681 atgatcggca ggcgaatcat cttgccaatc aagtgctggt atcgctcgtc gtcggggtgg
     1741 accgcgaccg cggtatcgcc gagcatcgtc tccggccgcg tggttgcgac gacgacgcgc
     1801 tcgtcgctgc cttcgaccgg gtaggcgata tgccagagat gacctttcac gtcgcgatgg
     1861 atgacctcga ggtccgagag cgcggtgtta caacgcggac accacgagat tagccgctcg
     1921 ccgcggtaga tcattccctt gtcatagaga tgcttgaagg tgcgccgcac cgctcgggac
     1981 gggcccgggt ccatcgtgaa cgtgaacctc gaccagtcgc acgacgcgcc aaggatccgc
     2041 atctgctcgc gaatccggcc gcggtactgg tccatgtact cccagacgcg ctcgaggaac
     2101 ctctcgcgac cgagatcgtg ccgggtcagt ccctccttcg cgatcagccg ctcaacgacc
     2161 cactggccgg caatcccggc gtgatccgcg ccgggcagcc aaagtgtcgg atcgccgagc
     2221 atgcggtgat accggaccat gatgtcttga atggtgacga agagcgcgtg accgatgtgg
     2281 agctcgcccg tcacattagg cggcggcatg atgacaacga acggttcgcg gtcagtccga
     2341 tcagacggct ggaaatagcc cgccgcatcc cactgatcgt agagaccctg ctcaacctct
     2401 tttgggttga acgcaggcgc caattgcacc gcctgatcgc gtcctgtcac cgcatttccc
     2461 tgttctggag cacattccgg ccaactgacc ggttctgaca attgtagggt ctgcacgacg
     2521 atttacgtag cgatagaggt gtccggacgt ggtaaacttc gtacaaacgt tcgtagcttc
     2581 atccccgcac tggctgccag caaagaagga ccccaatccc tatgccccag aacgaccgga
     2641 tcgttgtccg cggcgctcgg gaacacaacc tcaagaacat tgacgtcgag atcccccggg
     2701 acaagctcgt agtcttcacc gggctgtccg gctccgggaa gtcgtcgctt gccttcgaca
     2761 cgatctttgc ggaaggtcag cgtcgctatg tcgaatcgtt gtcggcctat gcgcggcagt
     2821 tcctcggaca aatggaaaaa cccgacattg accacatcga cggtctctcg ccggcgatct
     2881 cgatcgacca gaagggcacc agccggaacc cgcgctcgac cgtcggcacg gtgaccgaga
     2941 tctacgatta cctgcgcctg ctttatgcgc gagtcggtca cccccactgt ccgaattgcg
     3001 gccggcctat cagccaccag acggtgcagc agatggcggg cacgattctg ggcttgccgg
     3061 atggtacgcg cttgatgatc cttgcgcccg tgatcgagga ccgcaagggc gagtacctgg
     3121 ctgtcttcga tgagatccgt cggcagggct tcgttcgcgt tcgggtcgat ggcgaggtcc
     3181 gcgatcttga cgagacgatc acgctcgaga agaacaagaa gcacacaatt gaggtcgtag
     3241 tcgaccgagt catcgtccgg cacgatgagg gatcggagct ggacgacaac ccggatcgcg
     3301 tccgcatcat cgactcgctc gagaccgcgc tccggatcgg gaacggtcag gcagtcggcc
     3361 agatcatcga cggcgaggcg ctgcggttct ccgagaagtt tgcctgcgtg cactgcggac
     3421 tgagcttcgg tgaactcgag ccacgcaatt tctcattcaa ctcgccgcac ggcgcttgcc
     3481 cgacgtgtac tggtcttgga acccggctcg aattcgcgcc ggagctgatc gtgccgaacc
     3541 gcaacctttc gctggcccag ggagcgatca tgccctggat gcgggccggc ggcgacaaca
     3601 gcacctggta ctacgcgctc ctcaatgcga tgtgcgagaa gtacgggcaa tcgatggacg
     3661 tgccctactc ggagctgccg aaggatttcg tcgatctgct catgaacggt aacaaacaga
     3721 aagtgaccgt ccggtacaag tccggaagtg gtcgctggcg ctcgtacgac gtgccgttcg
     3781 agggtgtcat cccgaacctc cagcgccggc ttcagaacac gacgtccgac tacgtcctcg
     3841 aggagctggg acggtaccag gctgctcggc tttgcccgac ctgcaagggc gcgcggctca
     3901 agccggaggc gcgggcggtg ctggtcgatg gcaaggggat cgtcgagatc acgaccatgt
     3961 catgccggga cagtcgcgag ttcttcgatc gactctcagc cgatccggct gacgtgcccg
     4021 gcgctcccct gactgaacgc gagtacctga ttgcccggca gattctgaag gagattcgcg
     4081 agcgactggg atttttggtc gatgtcgggc tcgattacct gacccttgat cgatcagcca
     4141 ccacgctttc cggcggtgag gcgcagcgga tccgcctggc gacgcagatc gggtcgagcc
     4201 ttatgggcgt gctgtacatc cttgatgagc cgagcatcgg tctccatcag cgagacaacg
     4261 agcggctgat ccgcacgctc gaacgattgc gcgacatcgg caatacactc attgtcgtcg
     4321 agcacgacga ggatatgatg cgcgcggccg actggatcat cgatatcgga ccgggagccg
     4381 gcgtccacgg cgggcatgtg gttgcggccg ggacgatggc cgacatcgcg cgcagcccgg
     4441 agtcgatcac cggtcagtac ctgacgggcg agcggcgcat cgaggtgccg atgatgcggc
     4501 gaccgggcaa cggcaagttc ctcgagctgc ggggcgcgtc cgagaacaac ctgcaagacg
     4561 tcgacgtcaa aatcccgctc ggcgtcttcg tcgcggtaac tggcgtctcc ggatccggcc
     4621 agagctcgct cgtttcggac acgctgcaca agcgactggc gatcgacctc aacggcgcgc
     4681 gagagcgggc cggcgcgcat gatgccctct tcggcgtcga gcacctcgac aaggtgatcg
     4741 acatcgatca gagcccgatc ggtcggacgc cgcgatccaa tccggcaacg tatacaggcg
     4801 tcttcacgcc gatccgggag ctgtacgcgt cgatgcccga ggcgaaggcg cgcggctaca
     4861 agccgggccg attctcgttc aacgtcaagg gcggtcgctg cgaggcctgc tcgggcgagg
     4921 gcttgatcaa gatcgagatg aacttcctgc cggatgtcta cgtgccgtgc gaggtctgtc
     4981 acggcaagcg atataaccgc gaggcgctcg agatccacta caagggcaaa tcgatcgccg
     5041 acgtgctcgc gatgacggtc agcgagggcg tccagttctt cgagaacatc ccggcgatcc
     5101 ggaacaagct ccaaacgctg caggacgtcg gacttggata catcaccctc ggccaacccg
     5161 cgacccagct ctcgggcggt gaggcgcagc gcgtcaagct ggcctctgag ctctcgcggc
     5221 gggcgactgg caagacgatc tacatcctcg acgagccgac gacgggcctt cacttcgcag
     5281 acatctcgaa gctgctggat gtcctccagc gactgaccga cggcggcaac tctgtcatgg
     5341 tgatcgagca caatctcgac gtgatcaagt gcgcggactg gatcatcgac ctcggtcccg
     5401 aaggcggcaa cggcggtggc cggatcatcg ccgagggcac gccggaggcg gtcgcggcca
     5461 atccggagtc gttcacgggt gagtacctgc gccacgtcct cgaaccggcg gcggtcgcgg
     5521 ccgactaaat tacccaaata tattgaggta ttcggttacc ccgtgctacc atccatggtg
     5581 gtgcgaacta tcagtcaaaa gtaattgacc gtgtgcgagg gtcacctctt ggctggggag
     5641 ctaggtgaca ggcatcatga gtggcaccgt gctggtactg aaccagaact acgaaccgct
     5701 caatatctgt aacgtccgac gggcgatcgt ccttgtcatt gacggtaagg ccgaagtgct
     5761 ggagcagtac agcttcgcgc ttcatgctgc ctcgcgcgag tttccctttc cgtcggtaat
     5821 ccgcctcgtt tacatgatca agcggccgcg accgcgcgta aagttgaccc gccgtgagat
     5881 cttcatccgg gacgactata cgtgccagta ctgcggcaag cacacgagcg atttgaccat
     5941 cgaccacatc catccaaggt ccaagggtgg gccacatacg tgggacaacc tggtctcggc
     6001 ttgccgaagc tgcaaccacc gcaagggcgg gaaatcgctc gcggaggcgc gcatgcaact
     6061 caagcggcag ccgtttgagc cgaagcccgg cgcctactac accatccagc gtcgcgtaga
     6121 atcgggaatt cacccggact gggagaagtt cctgcccggg taccaggtcc tgatcgagcc
     6181 cgcacgcgca ttctccagtt agtcagcggc cgacggcgct cgatcgccgt ggccatattg
     6241 agacgtcccg gcagatggcg ggacgttcgt tttgtccaga gggagagcag accgtgatcg
     6301 acatccgcat catccgcgaa cggccagatg aggtgaaaca cgcccttgag ctgctcaata
     6361 cctccgcgcc gatcgaccgg atcatcgagc tcgatgcgga gcgacgccgc ctcgtcacgg
     6421 acgtcgacca gctcaaagcg acgcgcaacg aaggctcgaa ggcggtctcg aagaccaagg
     6481 acccggatga gcgcaatcgc ctgatcgccg agatgaagca ggtcagcgac tcgatcacgg
     6541 cgatggacgc gcggatcacg acggtagacc aggagttcaa cgacctgatg ctccaggtcc
     6601 cgaatatgcc gatgcccgag gtgccggtgg gcaaggatga cagcgaaaac gtagtcgtcc
     6661 ggaccgtcgg cgaaattcgg gagcgcgatt tcacggttcg accgcactgg gagaccggcg
     6721 tcgacctcgg catcatcgac ttcgagcgcg gcgtcaaggt cgccggctcg cggatgtatg
     6781 tcctcaaggg gcttggggct cggctgcaac gggcgatcat tacctggatg ctcgacctgc
     6841 acatcaagaa gcacgggtat agcgaggtct atccgccgta cctggtcacg tcggacacgc
     6901 tgatcggaac tggcaatctt ccgaagttcg gcgacaacct gttcaaggac tacctagagg
     6961 atcgctggat gattccgacg gccgaggtgc cagtcacgaa tctctatcgc gacgaaatcc
     7021 ttgacgaagg ccagttgccg atctatcacg ttgcctacac gccctgtttc cggcgagagc
     7081 agctttcggc cggtcgggat gttcgcggca tcaagcgcgg gtatcagttc gacaaggttg
     7141 agatggtcaa gttcgtccac cccgagcggt cgagcgcgga gcacgagcag ctgattcagg
     7201 aggcagccga tgttctagaa gcgctcgatc tgccgtaccg agtggtccag atgtgtaccg
     7261 gtgacctttc gtttacggcc gcgaacaagc tggacatcga agtttgggcg cccggttcgc
     7321 aagagtggct agaggtctcg tcctgctcga acttccgcga tttccaggcg cgacgggcca
     7381 accttcgctt ccgcccggaa ggcggcggtc ggccgcaata tctgcacacg ctcaatggat
     7441 cgggcctggc ccttccgcgc accatgatcg cgatcatgga gaactaccag aactcgaacg
     7501 gctcattcga gatcccggag gtcctgctgc cctacatgga cggcgtgacc cggatctccg
     7561 gagcctaggc tcgcaccttc tcgcggtccg gatcgtatgc agcgcgctgg ttgaccatcc
     7621 cggtcgcctg cgcgccggtc ttgtcggtga cagtgacccg cgtgcccgtc gtggccgcgt
     7681 ccggttgcag gtacgcgagc gcaatcgatt tgccgacgaa gaagccacgg tcggccgaag
     7741 tgaccttgcc gacggctgaa ccgtcgattg agaccgcatc gcccgtcgca ggcaggaaat
     7801 cgagcccgtc gaactcgatc gcaaccaggt tgcggtcgac gcccttctcc ttctgccgaa
     7861 ggagcgcatc ccggccgttg aagtcgccct ttccgaaccg cactgtccag ccgaggtttg
     7921 cctcgatcgg gctggtcgtc tcatcgaggt cgagcccgta gaggggatat cgcttctcca
     7981 tccggatcga gcggagcgcg ccgagaccgc atgggaccag gccgtgctcc ttgccggcgt
     8041 tgtagaccga gtcccacatg tagatcccgt actcgaccgg gtagaagaac tcgaatccga
     8101 gctcgccgga gtagccggtt cgcgcgagat atccaggaac ttccgccagg gtcgtctttg
     8161 taaacgtgta atacgggagc gcgtcggtcg agagatcggc gtcggtcact tggctcacga
     8221 tttcgcgcgc gttcggacct tgcaccgaga tgaagccggt acccgagacc cgatcggtca
     8281 catacgcatt catcccgcgg gcctgctcgg tcaagtgcgc cacaatcgcc tcgacgcgag
     8341 atggggtcgg gctcaggaag aagtgatcgg gcgcaatccg gtagatcgtc aggtcgtcga
     8401 tcatcccgcc gtcatccttg cagaccgacg agtacagaac gtgaccatcg acgatgcggg
     8461 tcaagtcatt gacacacgcg ttctggagga gcgcctcggc gtccttcccc tttatgtcca
     8521 ccattccctg gtggtagacg tcgtacaggc cggcggcggt tcgggtcgcc atatgctcgg
     8581 cctcggtggt cgagaagcgg tatggcgcgt cgtagcccat ccgatcgacg agctcagcac
     8641 caagctcgac gtacttcgcg taatacggag atctccgccg tcccatgctc actccctcac
     8701 ccggcaagcc acactcggcc ccgcccgcat acaaaaggca atccactcgc gtgccgggta
     8761 ctatacgaga cggatttgcg agccatcggg acggttcata tactggagtt gctggagagg
     8821 tggccgagtg gtttaaggcg gctgtcttga aaacagttgg gtgatgagcc ccgggggttc
     8881 gaatccctcc ctctccgccc ctccccgttc cgttcccgca caagaaatcg ctcagggccc
     8941 gctgtcgccg tgcggcgtgc gcgatactcg tctgctggag aagtcatggc cgttgccact
     9001 gatacccgcg ttgatgccta cattgagcat gcgcccgaat tcgcgcggcc gatcctggtc
     9061 aagcttcggg agcttgtgca tatcgcctgc ccggacgtga ccgagacaat caaatggggc
     9121 gtgccggcgt atgaatacga gggcatcctc gcgatcatgg cctcgatgaa gcggcatgcc
     9181 gtcttcaacc tctggaaggg cgaattgatt cccgaggtca aggagctcta cagcgaaaag
     9241 ttcagcatgg cgatgggcac gttcggcaag atcacctcgg tcgatgatct gccgccggat
     9301 gacaagatca tcagttggat tcacgcggcg atggacctca acgagcgcgg cgtcaagctg
     9361 ccgcagcgat ctcgaaccga gccgaggccg gaggcggagg tgccggacga tctggtcgcg
     9421 gccctcgagc agaacgagcc ggcgcggctg accttcgaag ggttccctcc gagccaccgt
     9481 cgggactatg tggagtggat caccgaggcg aagaccggtg ccacccgtca aaagcggctg
     9541 acccaggctg tcgaatggat ggctgggggc aagcaccgca actggaaata cgacaagaag
     9601 aagtagctgg aatcatgact gacgacatca cgattcgtcc ggaacggccg gacgacgttc
     9661 aagcgatcca tgacctcgtc cgggaggcgt tcggccggga actcgaggcg gatgtggtcg
     9721 cgaagctgcg cgagtctgct ggattcctcg ccgacctctc gcttgttgcg gtgcgggaca
     9781 gccaagtggt cgggcacatc atgattaccg agctcgacta cccatccgat gccggtggcc
     9841 gagtgcagtc cacgattctt gctccgctgg cggtattgcc gcactttcag aaccagggta
     9901 tcggagatgc gctgagtcgg gtcgcgctcg agcgcaccaa ggaggccggc tatggctcga
     9961 tgatcctcgt cgggcacccg aattactacc cgcggttcgg cttccgaccg gcatcgaact
    10021 ggggcattcg atatgcgact ccgatcccgg atgacgtatt catggcggtt gagctcgtgc
    10081 ctggatcact cgacgatgcc gccgggacgg tgatattgcc aagcgcgttc gacgaagcgt
    10141 gacccggccc acgtaggcgg atcggcaagc gagcgcagca catcgatcag gcaccccgca
    10201 cgcatggccg acgaggcgga tttgggaccc tggatgcggt acaataccag gcgacctggc
    10261 caatggatcc ggggaggtgc cagagtggtc gaatggggcc gcctgctaag cggttgaggt
    10321 gccctaaagc gcctccgagg gttcgaatcc ctccctcccc gccccggtat ctgccttggt
    10381 cttcctccga ccgaactgat gaaaccaggc gactgtcaat cgttggcagt tctgtcctgg
    10441 atgtcgcgat tttcgccggg tgagggcgtg ccaaggacca cgtccgcgag gttgtaatcg
    10501 tggaagcgtc catacagtcc gcgcccgcgt cgcatcagac gcatcgccat cgctcccacc
    10561 agatcgagta cctcctcatc ctccagcgcc gcttccaggc cgtccagatc gtcgaaaccg
    10621 acctgataaa ccggtcctac ggtcttgtac caggcagatg ctacgaagac gccccgcgat
    10681 tgcacatctc gcgtcagaag cttatccggg tgccgctcgc tcgggttggc cgacatgaaa
    10741 cccagcgtcg tccagcactt gccgtcgaac tgttcgccta tcgtcaacgc ctcgacgttg
    10801 ttgatcgaca ggcaagcgag ccgatgccac aagcgagtct ttgcggtcga cggcaatgag
    10861 gtgatgaccc aatgggcgac ttcagtcgat gccggcgcgg gcacgaagag gtcgagatag
    10921 cgagccacga gtgtggcgat gcgctcaaaa tcaggatgtt tgctgagacg atcgaagagc
    10981 ggtcgtgtgc gctcccgttg atccggacgc tcgatcgggc gcgcgtcctg ccgccccagc
    11041 aagaggtcat cgctccactg cattcgatcg agtaccgggt caatttcggg cgcctcggca
    11101 tcaacttttc gcaacggcgg acttggtcgc gccactctca ccggactgcg gtcggccaaa
    11161 gcagatcgtt cctcgcagtc cttcccgggc cagaatctga tggcccgcat gtcgtcccag
    11221 gtccgtccgt ggctcccaag gcggagaacg atgttgtgag tctcgccgac gtagcactcg
    11281 ccgttcgtga attccacgcg atacgtgccg gaccttggtg tccccaatgg cagcgatggg
    11341 ccgcgtcgcc gtagatcggt cgcgtcgatc tcaatttcgg gcgcaggacc gggcatgcgg
    11401 gcgaccttct ctctcacaat ctgctggttc ggtggtagaa tatccgtcgc gcgttacgcg
    11461 tgcgcctgta gctcagggga tagagcgttg ggttgcggac ccaaaggccg caggttcgaa
    11521 tcctgccagg cgtacccctc catcaaaccc gtctgaaacc gccaatccgg acgttcggta
    11581 tctggtcacg gccggagcag gtttgctaaa ctgacagacg gagaggtgtc cgagtggttg
    11641 atggtgccgc tctcgaaaag cggtgtagtg atgagctacc gtgggttcga atcccaccct
    11701 ctccgccggc cgttcgggac tgtcccaacg gccgcttctc ttggaagggt cgcatagcct
    11761 ggcctagtgc gcgcgactgg aaatcgcgta gacggtaata ccgtctcgag ggttcgaatc
    11821 cctccccttc cgccgtgccc caaccaattc cccgctttcc gatccccgcg ctggcatcgg
    11881 cacatgacac cgcgtccggc aaccgcgtca ggcgcctgag taagccaggg agacaccgtg
    11941 cgaatcgcgt tcaccagcat tcttgtagac gatcaggaca gggcgctggc cttctatacc
    12001 gacatgctcg gtttcttgcc aaagaccgat ttcccggtcg gagacttccg ctggattacc
    12061 gtcgtctcac cggatgcgcc tgacggcacg gagctggtgc tcgagccgaa cgtcaatccg
    12121 gccgccctgg catttcagac cgccatgcac gagcagggca tcccgctcac ggcattccag
    12181 gtcgacgaca ttgccgggga gtacgctcgc ctccgcgaaa agggcgtcgt tttcacgcag
    12241 gagccaactg aagcgggacc ggtcacgatc gcactcttcg acgacacctg cgggaacctg
    12301 atccagattt tccagctcaa cggataggac tgctacttca ccgcggccag gaggtcgtcg
    12361 atttgcttcg tggtcggcat cgatcgctgt gcgccgtcga tcgtgcaggc gaccgagccg
    12421 gccgcaacac cccgtcgaat ggcctcattc ggcgttgaac cggcggcaag tgccgccgca
    12481 aacgcaccgc acagcgcatc gcccgcacca gtcgtgtccg taaccctgac cgccggcgcc
    12541 gggatcgcga cccgctcgcc ctcgaacgac gcaacggcgc cctctggtcc gattgtgatg
    12601 atcacccagc gcggtccttg ctcgcggagc atctcggccg cggcaaacca gtcggccggc
    12661 gcctggcggt ggagcagcgc catcgcttcg ggctcattga cgatcaggca gtcgatgtcg
    12721 cccagaaacc gcgccgctcc ttccggctcc ggcgtcgcat tgagcaggac gagtccatgc
    12781 cgaatccgga ccgcctcgat tgcggccgca atggactcgg ccggcagctc gagcgtggtg
    12841 aggagcacgc gaggagtcaa cgtgtccacg gccgatcgcg cctcctcagg agtcacggtc
    12901 atggttgcgc cgggaacgta ggcgatacga ttctggccag acgagtcctc gacggtaatc
    12961 agcgtcactc cggacggcgc atcat
//
LOCUS       DATAOZ010000048        12742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6862525, whole genome shotgun sequence.
ACCESSION   DATAOZ010000048 DATAOZ010000000
VERSION     DATAOZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6862525"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VHR64_01410"
     CDS             complement(<1..1091)
                     /locus_tag="VHR64_01410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3301505.1"
                     /translation="MTQPAATTSRAAARTASVGLIAGTLGAIGLLDGGGGGTWTDVLA
                     SFGLSDSTLGPAFATQSAFVFPVLLFGGIVLSRIGLRLMLVIGLLLLATTSFAMTGFE
                     GLALFLLLFAIRGTGIALLDLAGNTMAMQVERETGRHIMGIVHGGFSVGIIIGSLMAF
                     GIYAVGGGFRLIHGLLGIALVVLAVAGALGPIPHVESEATPQRVSLRAFNSRMVRIAA
                     IGLGLAFGGEVLISQFVSVLLRTRIEASDATSVLAVVIYATMMAVGRFGNGPLMNRFD
                     AITLVYVQGAVMAVGGAVIATSTSVPMTLAGSFIGGIGVAGIVPTILSYAAAHTRTSP
                     GETAGAALLGGYFGGFIMPLLAGGLTSVLS"
     gene            complement(1088..2098)
                     /locus_tag="VHR64_01415"
     CDS             complement(1088..2098)
                     /locus_tag="VHR64_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3301506.1"
                     /translation="MTERDVYDITIIGAGPTGLFAAFYAGMRQARTQIIDSLEEPGGA
                     LTAIYPEKYIYDVPGFPKVMAKDFVEQMVLQAERDQPTMRLSEEVLNLERLEDGIICL
                     TTTKCTRFSKTVIICAGVGAFEPKRIDAPGVADLEGKGVHYFAKKVDDFRERDVVIVG
                     GGDSAVDWAVTLEPVASHVTLIHRSKFRAHEATVRELEEGRVELRYPGCEIVEVHGAN
                     EGYLHAVSYKDATGEVFRIPADDLIVAIGFLADLGPLKTWGLSLSDRRNQVVVDKITM
                     ETNIPGVYAAGDIASYPAKFKLIANGAAEAVTAVNHAVTFFDPGARLDAGHSTNIMAS
                     RE"
     gene            2274..3482
                     /locus_tag="VHR64_01420"
     CDS             2274..3482
                     /locus_tag="VHR64_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3301507.1"
                     /translation="MERDYAAYVSEKGRRAGDALITDPTASANYISFIYGFPDSESLP
                     AGSVTETTHRVLTENPVWALQYGAVNGPKPIREAILAKLKRDQGISASMDEIIVTAGS
                     SQAIQLITQLLVDPGDTIIAESPTFLGFFDDVRNSGANITGVEVDEEGIRVDLLEQEL
                     VALRENGVRPRFLYILPNYQNPTGVCTTLERRKRIVELAEEFDTLIIEDDAYFDLRYE
                     GEALPTIYSLDPHGRTLYLGTLSKTLAAGFRIGWVIGPAPIIRRIASLKTDGGTNIFG
                     SFIAASWLPEHFERHVDELRRIYGQRRTLMLAALEKYMPVGATWSVPTGGFFVWLTLP
                     EGVDSGPMLVQAREMGIEYLPGTACFFDHTGANHIRLSFSFARDEVIDEGIRRLGELI
                     RSELAESISG"
     gene            3600..5723
                     /locus_tag="VHR64_01425"
     CDS             3600..5723
                     /locus_tag="VHR64_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3301508.1"
                     /translation="MNTRLISRQAMRDQLPRLRELERSARSTIGETLRNRWRDQRPAG
                     AGAGLPDPVFEIIVGTVSVLVPVLILLPLHDLMEPTLAAVILMVGVALATHLGNWLGG
                     ITAITLTLLSIDLFWIGDSFTEVMPDQAAEVVSLVVIGAAGLILAWLIQEVKGQSESA
                     RRDAQAARSATFALNSIEADAAAYARGGIGNRSMIYGSLLRAMVAANRTAFGVLLLVE
                     DEHYPYAVAGYGLEVTDAEELAPEYLDEILEQRRPRTIYDVNLDFRFDDSEFRRAGVR
                     SLLGVPMFGGQDQIVGIVITGLHAAHSFTSAEVYRLTALTDKAASILEALEAVDEREL
                     ALRHAEDRQVWLERVMGAIPEAMMLIDHETGAILAQNPVAAALLGELSGHHVSQAFAR
                     LRTVDGEYVTAASSPIVNAVEDLEVVSGTEMLAIRPDGRQIPVLVSAAPVRHPGDPMH
                     GVVTVFREISALKEAARLKDEFVSVVSHELRSPLTPIRGFVQLVARDLSRKGGNEDHV
                     QRLNSAAAHVDRMTRLVDDLLDVSRLKAGLLDLRRSPVNLADICFEVITDRQAGGVKQ
                     AIKLDELPSDVVGEWDADRLYQVIDNLVGNAIKYSPPDGIVRISMCEDRDNGSASVTI
                     ADEGPGISADEREQVFSAFFRTKSAAASQVSGLGLGLYICSELVHAHGGEISVHESDS
                     GGAAFRVTLPVVARAEQALEPAAAS"
     gene            complement(5700..7697)
                     /locus_tag="VHR64_01430"
     CDS             complement(5700..7697)
                     /locus_tag="VHR64_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301509.1"
                     /translation="MLKRSITAWLLVAVMLLSVVLAAARPGAVRSAPEPRITTQAVAY
                     QTLPDWMPPETAAQLDIGIDVLVPLSIPAPFSGVPQVSANPGYYSLYWFVGGGTPTLL
                     QITGTAGGDIPAYSKYDRNVQLVANASVNGATAYRDLTPIYDLVYWKVGNVVYSVESQ
                     NSSVDSLTIANGLTVLAVPASSSPRTATVFSPDQITAGDVGNVTVSVSGDATLSTDVG
                     YFTANGQSSISVSGDETVEWQAPTLTDPVTATFQVIDPSDGSVLASTPTQIVVTQSTS
                     TEWGLDCPPRGVAGDVVTVTASGSQRATLSASDGSFTGGSPAIVLNLDGSTDVDFTMP
                     FDGGTTARLTLSDGESAVATCEIEVVAEENSEPTATATKTPKGIFPGDATDLSIPTGK
                     DPALPKDFGTPTPVATVDPSKLPGDGTGIVEAGQVTPPAVPPTATPTKTPKPGEPTST
                     PVPTETPVPTLTPTPENPMPTMVPQVGNEGRLVALEMGPAGGSLNSPYGVQIVVPENT
                     FSDTTSVTLQPVADNQVPLQAGVSLVPESAYEISFAKLNGRGINLGDKEVTVRVDLGE
                     RWADGATLFQLVNGQATPLRNVKVDGTALEGKINGPTRVVAGVPTAQIAGGSRSLVPI
                     VIVALVAVILLIVAGSILISLRNRRPRTVATRRTLGSRGRF"
     gene            7872..8222
                     /locus_tag="VHR64_01435"
     CDS             7872..8222
                     /locus_tag="VHR64_01435"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX3301510.1"
                     /translation="MLVRIIAYSIGSIVAILAVGTIFTSDLVDYVDERSVFIFGIVLG
                     LMTALLKPILSALTLPISCLTFGLFALVLNAAMFGAAAWLTPDVEVTALGALVGGIFV
                     AVINGVLYSVVDQS"
     gene            8237..8824
                     /locus_tag="VHR64_01440"
     CDS             8237..8824
                     /locus_tag="VHR64_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX3301511.1"
                     /translation="MFVEIGTIQVKLPARCRLALIADTHIYPSGQRSIPEPVLDLFDR
                     AGVDRVIHAGDIACAPVLDQLATIAPVIAVRGNNDDGDFGRSLPHMVEMQVGQRLVRL
                     IHGDGGRSARAVATDVAPGADCVIYGHSHIPKVERAGDAILVNPGSPNDRRWHPHFGV
                     GFLEVTPDLVRPELILFTDPTDLARVSPTWRSDLK"
     gene            complement(8810..10156)
                     /locus_tag="VHR64_01445"
     CDS             complement(8810..10156)
                     /locus_tag="VHR64_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3301512.1"
                     /translation="MEVSLSPRGRTIVIWIGVIAGAVLLLEAANALRPFAWAVITAYL
                     LHPFVSMIHRRTRLPKPLITLWLYAMIGLLLTILAINFGPVLVDQISQFKDEIPKFSD
                     DIETWINDNQQGRLDEMGIDSSYVNERLDEVGTEIANTLGKAALPVLFSTFSIAIELL
                     IYLVASFYFIVHGDRFVMAFRAVLNRKYHAEFDRLLAEINATLGAYLRGQALLVLIMS
                     TASFAVLSVLGVKYALIIAIATGFLELIPLVGPWIAGAIAVTVSIFQDTTPFGWSHVG
                     LAVVIGLAYFALRQAEDAFVIPTVIGRFVHLHPLLVIFCVVVGTALGGILGLILAVPI
                     AAVLKILISYLYAKLITREHRHVEVIQSREQLEYLVNQFPLMTNSSVVLLIEPNVLLW
                     ENLPLVRSISDAAYEHSVGLKAVTPDGIAGTLLHAVGIETTTVPSVSPIPAPSLQV"
     gene            10326..11438
                     /locus_tag="VHR64_01450"
     CDS             10326..11438
                     /locus_tag="VHR64_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX3301513.1"
                     /translation="MDFDQFESLNFRLPGPTPLPPSVRAAMDRPAIHHRGPLLKSTMR
                     SILARLKEIHRTEQDLLVWPGSGSAGWEIAITNLLCPGDHVVVTVCGDFGERFASVAG
                     KLGLVVHRVEKPWGEAIRPSELKGVVECVPECQAVLITHNETSTGVTNPLPELAAVAR
                     EAGALTIVDAVSSASALPLEVDSWDLDFVISGSQKAWMCPPGLVICAIGKRAWSAYDA
                     STYPRFFWDITSARKSAAEGMTPTTPPLHLLFALDAALDLMVDEGVEAMWARHHALGE
                     YARGRVRELGLELLAEPDFASDSLTAIRVPEGLSARAIIDAMVANHRVMLQAGQGAMT
                     DQVLRVGHMGWVTERDLEEAFDALGATVATSEFRAR"
     gene            11613..>12742
                     /gene="rpsA"
                     /locus_tag="VHR64_01455"
     CDS             11613..>12742
                     /gene="rpsA"
                     /locus_tag="VHR64_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX3301514.1"
                     /translation="MSGTDESTQTLSLMEEFLNDPSLEYKSLKYGDVMDGVIMRIDRD
                     EILVDIGSKSEGIVPSREFSSLNSEEKGELSIGDTVLVFVVQPENQEGQAVVSIDRAR
                     QEKSWRRLQELYEANESIQAEVTNYNKGGLLVNLDGVRGFVPASQVTEIRGGDDASKQ
                     ADMAKLIGTKLNLKVIEINRHRNRLILSERQAMQERRDEMKEKLIEELKEGEVRHGRV
                     SSICDFGAFVDIGGADGLVHLSELSWSRVRHPSEVLKVGQEVDVAVLGINPQERKIAL
                     SLKRTQAEPWSQVGDKYEVGQLVRGTITQLANFGAFARIEDGIEGLIHVSELTDERIT
                     HPKQIVKEGDELILRIIRIDPQRRRMGLSLRRALDTPDQELMA"
BASE COUNT         2294 a         3843 c         4243 g         2362 t
ORIGIN      
        1 gagagcaccg acgttagtcc gccggcaagg agcggcatga tgaagccgcc gaagtacccg
       61 ccgagcaagg cggccccggc ggtctcgccg ggtgacgtgc gggtatgtgc ggcggcatag
      121 ctcaggatcg tggggacgat accggcgacg ccgatcccgc cgatgaacga cccggccaac
      181 gtcatcggca cactcgtcga cgttgcaatg accgcgccgc cgactgccat caccgcgccc
      241 tgaacgtaga caagcgtgat cgcgtcgaac cggttcatca acggtccatt gccgaagcgg
      301 ccgacggcca tcatcgtggc gtatatgacg accgcgagga cactggtcgc gtccgatgcc
      361 tcgatcctgg ttcgcagcag cacgctcacg aactgtgata tgaggacctc gccgccgaac
      421 gcgagaccga ggccgatcgc cgcgatccgc accatccgcg agttgaacgc gcgcagtgag
      481 acgcgctgcg gcgtggcttc tgactcgaca tggggaattg ggccgagtgc ccctgcgact
      541 gccaacacga ccagggcgat gcctagcaaa ccgtggatca gccggaaccc gccgccgacc
      601 gcgtagatcc cgaacgccat caatgagccg atgatgatgc cgacgctgaa gccgccatgg
      661 acgatgccca tgatgtgccg gccggtttcg cgctcgacct gcatcgccat cgtgttgccg
      721 gccaggtcga gcaaggcgat gccggtaccg cggatggcga agagcaggag gaagagcgcc
      781 aatccttcga aaccggtcat cgcgaatgat gtcgtggcga gcaggagcag accgatgacg
      841 agcatcagcc gcagtccgat ccggctgagt acgatccccc cgaagaggag aactgggaac
      901 acgaaggcgg attgggttgc aaacgcgggt cccagcgtcg aatcgctcaa cccgaagctg
      961 gcgaggacgt cggtccatgt gccgccaccg ccgccatcga gaaggccgat tgctccgaga
     1021 gtgccggcga tcaggccgac ggatgcggtt cgggcggcgg ctcgcgatgt tgtcgccgcg
     1081 ggttgggtca ctcgcgcgag gccatgatgt tggtggaatg gccggcgtcg agtcgagcgc
     1141 ctggatcgaa gaacgtcacc gcgtggttga cggccgttac ggcttcggcc gcgccgttcg
     1201 cgatcagctt gaacttggcc gggtaggagg cgatatcgcc tgccgcgtag acgcccggga
     1261 tgttcgtctc catcgtgatc ttgtccacta cgacctggtt gcggcggtcc gacagggaca
     1321 ggccccaggt tttgagtggc ccaagatcgg ccaggaagcc gatcgccacg atcaggtcat
     1381 cggccgggat gcggaagacc tcgccggtcg catccttgta gctgactgcg tggaggtagc
     1441 cctcgttcgc gccgtgcacc tcgacgatct cgcagccggg atagcgcagc tcaacgcggc
     1501 cctcttcaag ttcgcgtacc gtggcctcat gagcccggaa cttcgagcga tggataagcg
     1561 tgacgtggct ggcgacgggc tcgagcgtga cggcccagtc gaccgcagaa tctccgccgc
     1621 cgacgataac cacgtcccgt tcccggaaat cgtcgacctt tttggcgaaa tagtggacac
     1681 ccttgccttc cagatcggcc actcccggtg cgtcgatccg cttcggctcg aacgctccaa
     1741 cgccggcgca gatgatgacc gtcttgctga accgggtgca tttcgtggtc gtcaggcaga
     1801 tgatgccgtc ttcgagccgc tcgagattga gaacttcctc gctcagacgc atcgtgggct
     1861 ggtcgcgttc ggcctggagg accatctgtt cgacgaagtc cttagccatg accttgggga
     1921 agcccgggac gtcgtagatg tacttctcgg ggtagatggc ggtcaaggcg ccgcctggct
     1981 cttcgagcga gtcgatgatt tgcgtccgtg cctgacgcat accggcataa aacgcggcga
     2041 acagcccggt cggtccggca ccgatgatgg tgatgtcgta gacatcgcgc tcagtcatag
     2101 cgcacatgat ctccagggtt gagcaacggg caaagccaaa tgttcagggg aacggcgatg
     2161 ttcgtcgcga aagtctagca ttgcccttcc aggcgagcgg acctgacagg ccgtagaatg
     2221 cccggatcaa attgctgtat cgaaggggta tcggaatcca aatggtgagt caggtggagc
     2281 gggattacgc ggcgtatgtg tcggagaagg gtcgccgggc gggtgacgcg ctcattactg
     2341 acccgacagc gtcggccaat tacatctcgt tcatttacgg ctttccggac tccgagtcgc
     2401 tcccggcggg atcggtgacg gagacgacgc accgcgtgct gaccgagaac ccggtctggg
     2461 cgttgcagta tggtgcggtc aatggaccga agccgatccg cgaggcgatc ctggccaagc
     2521 tgaagcgcga tcagggcata tcggcatcga tggacgagat tatcgtcacc gccgggtcgt
     2581 cgcaggctat ccagctcatc acccagctcc tggtcgatcc gggtgacacg atcatcgccg
     2641 agagcccgac cttccttggc ttcttcgacg acgtacgcaa ttccggcgcc aacatcaccg
     2701 gggtcgaggt ggacgaagag gggatccgcg tcgatctcct cgaacaagaa ctcgttgcat
     2761 tgcgcgagaa tggcgtccga ccgcgcttcc tctacatctt gccgaattac cagaacccga
     2821 ccggagtttg cacgacgctc gagcggcgga agcggatcgt cgagctggcc gaggaattcg
     2881 acacgctgat catcgaggat gacgcctatt tcgacctgcg gtacgagggc gaagcactgc
     2941 cgacgatcta ttcgctcgat ccgcatgggc gcacgctcta tctcggcact ttgtcgaaga
     3001 cgctggccgc cggattccgg atcggctggg tgatcggccc ggcgccgatc atccgccgga
     3061 ttgcgtcgct caagacagac ggcggcacga acatttttgg ctccttcatc gccgcttcat
     3121 ggctgccgga gcacttcgag cgccatgtcg acgagcttcg tcgcatctac ggccagcgcc
     3181 ggacactgat gcttgccgcg ctcgagaagt acatgccggt gggtgcgacg tggtcagtcc
     3241 cgaccggcgg attcttcgtc tggctgacgc tgccggaggg tgttgattcc ggccccatgc
     3301 tggtccaggc gcgcgagatg ggcatcgagt atctgccggg tacggcgtgc tttttcgatc
     3361 acacgggcgc gaatcacatc cggctatcgt tctcgttcgc gcgggacgag gtgatcgacg
     3421 agggaatccg gcggctgggc gagttgattc gatcggagct ggctgaatcg atttccggct
     3481 agaaaatcgg gcactttgac acctgtcaaa ggtgtacgta tgctcagtaa tcaatacgcc
     3541 tgaattgcgc caactcaggt ggactgctga tcgctgaccg gcgtgcggta ttgcagacca
     3601 tgaacacccg cctgatttca cgccaggcca tgcgtgacca gctaccccgg ttgcgggaac
     3661 tggagcgctc tgcgcggtcg acgatcgggg agacgctccg caatcgctgg cgtgatcaac
     3721 gcccggcggg tgccggtgcg ggcctgcctg atcccgtctt cgaaattatc gtcgggacag
     3781 tcagcgtact ggtcccggtc ctgatcctgc tgcccctgca tgacctgatg gagccgacgc
     3841 ttgcggccgt gattttgatg gtcggcgttg cgttggccac ccatctcggc aactggctcg
     3901 gcggcatcac cgcgattacg ctcaccctgc tctcgatcga cttgttctgg atcggcgact
     3961 cgttcaccga agtcatgccg gatcaggcgg ccgaggtcgt ttccctcgtc gttatcggtg
     4021 ccgcggggtt gatcctggcc tggctgatcc aggaggtgaa aggtcagagc gagagcgcgc
     4081 gccgcgacgc ccaggcggcg cgatcggcta cgttcgctct gaatagcatc gaggcggatg
     4141 cggcggcgta tgcccgcggt ggcatcggca accgatcgat gatctacggc tcgttgctgc
     4201 gcgcgatggt ggcggccaac cgaaccgcat tcggtgtgct cttgctggtg gaggacgagc
     4261 actaccccta cgcggttgcc ggctacgggc tcgaagtcac ggatgccgaa gagcttgctc
     4321 cggaatacct ggacgaaatc ctcgagcagc ggcgcccgcg gacgatctac gacgtcaacc
     4381 tcgatttccg cttcgacgac agcgagtttc ggcgagcagg tgtccgctcg ctgctcggcg
     4441 ttccgatgtt cgggggacag gaccagatcg tggggatcgt gattaccggt ctccatgcgg
     4501 cgcactcatt tacctccgcc gaggtctacc ggctaaccgc actgaccgac aaggcggcgt
     4561 caattctgga ggcactggag gcggttgacg agcgggagct ggcattgcgg cacgcggagg
     4621 accgccaggt ctggctggag cgcgtgatgg gcgcgatccc cgaagcgatg atgctgatcg
     4681 accatgagac aggcgcgatt ctcgcccaga acccggtggc agccgcgctc ctcggcgagc
     4741 tctccggcca tcacgtcagc caggcgttcg cgcggctgcg gacggtcgac ggcgagtatg
     4801 tgaccgcagc ctcgtcgccg atcgtgaacg cggtcgagga cctcgaagtg gtctccggca
     4861 cagagatgct tgcgattcgc ccggacggtc gccagattcc ggtgctggta agcgcggcgc
     4921 cggtacgcca tcccggcgat cccatgcacg gagtcgtgac ggtattccgg gaaatcagcg
     4981 cgttgaagga agcggcccgg ctcaaggacg agttcgtttc ggtcgtttcg cacgagctgc
     5041 gttcgccgct gacgccgatc cgcggtttcg tccaactcgt tgcgcgggat ctgagccgga
     5101 agggcggcaa tgaggaccat gtccagcggt tgaactcagc tgccgcgcac gtggaccgga
     5161 tgacgcgcct tgtggacgac ctgctggatg tctcgcgcct gaaggctggc cttctggatc
     5221 tgcggcgatc tccggttaac ctggccgata tttgcttcga ggtgataacc gatcggcagg
     5281 caggtggtgt caagcaggcg atcaagcttg atgaactgcc aagcgatgtt gtcggcgagt
     5341 gggatgcaga tcgtctctac caggtcatcg acaaccttgt gggcaacgcc atcaagtaca
     5401 gcccgccgga cggcatcgtg cgaatttcga tgtgcgagga tcgggataac ggatcggcct
     5461 cggtgacgat cgccgacgag ggaccgggaa tttcggccga cgagcgggag caggtcttct
     5521 ccgcattctt ccgcaccaag tcggcggcgg cgagccaggt ttcgggtctc ggtctcgggc
     5581 tctatatctg ctctgagctg gtacacgcgc acggaggcga gatttcggtg cacgagtctg
     5641 attcgggcgg cgctgcgttt cgagtcacgt tgcccgtggt cgcccgggca gaacaggcgc
     5701 tagaaccggc cgcggcttcc tagcgtgcgg cgggtggcga ccgtccgcgg ccgccggttg
     5761 cggagcgaga tcaggatact cccggcgacg atcagcagga tgaccgccac cagtgccacg
     5821 atgacgatcg gcaccaggct ccggctgcct cctgcgatct gtgccgtcgg cacaccagcc
     5881 acgacgcggg tgggtccgtt gatcttgcct tccagggccg tgccatcgac cttgacgttc
     5941 ctgagcgggg tcgcttgccc gttgacgagc tgaaagagcg tggctccatc ggcccagcgt
     6001 tcgccgaggt cgacccgaac cgtgacttcc ttgtcgccaa ggtttatgcc cctgccattc
     6061 aacttggcga acgagatttc gtatgcgctt tccgggacga ggctgacgcc cgcctggagc
     6121 ggcacctgat tgtcggcaac cggctgaagc gtgacgctcg tcgtgtcact gaacgtgttc
     6181 tccgggacga caatctggac gccataaggg ctattgagcg agccgcctgc cggtcccatc
     6241 tcaagcgcaa ccaggcgacc ttcgttgccg acttgcggga ccatggtcgg catcgggttc
     6301 tcgggcgtcg gcgtcaacgt cggcaccggt gtttctgttg gcacgggcgt cgaggtcggc
     6361 tcgcccggct tcggcgtctt cgtcggggtt gccgtcggtg ggacggccgg tggcgtgacc
     6421 tggcctgcct cgacgatgcc ggtcccatcg ccgggcagct tgctggggtc gaccgttgcg
     6481 accggagtcg gcgtgccgaa gtccttcggc aacgccgggt ccttgccggt tgggatcgag
     6541 aggtcggtgg cgtcgcccgg gaagatgccc ttcggcgttt tggtcgcggt tgcggttggc
     6601 tcggagtttt cctccgcgac gacctcgatc tcgcacgtcg cgaccgccga ttcgccgtca
     6661 gagagcgtca ggcgtgccgt ggtccctccg tcgaacggca tcgtgaagtc gacatcggtg
     6721 gatccatcga ggttgagcac gatggccggg ctgccgccgg tgaacgatcc gtcgctggcc
     6781 gagagggtcg cgcgctggga gccgctggcc gtgacggtga cgacgtcgcc cgcgacacct
     6841 ctcggcgggc agtcaagacc ccactcggtt gacgtcgatt gggtgacgac aatttgggtc
     6901 ggcgtcgacg ctaacaccga gccatctgac ggatcgatta cctgaaatgt ggccgtgacc
     6961 ggatcggtca gggtcggcgc ttgccattcg acggtctcgt cgccgctgac cgaaatgctc
     7021 gactggccgt tggcggtgaa atagccgacg tccgtgctca gcgtggcgtc gccggagacg
     7081 ctgacggtca cgtttccgac atcgcctgcg gtgatctgat cgggtgagaa gaccgtggcg
     7141 gtcctggggc tggagctggc cggaaccgcg agaacggtca ggccgttggc gatcgtgagt
     7201 gaatcgaccg acgagttctg actctcgacc gagtagacga cattgccgac cttccaatag
     7261 acaaggtcgt agatcggcgt caggtcgcga tacgcggtgg cgccattgac cgaagcattc
     7321 gcgacaagct gcacgttacg gtcgtatttc gagtaagcgg gaatgtcgcc accggcagtc
     7381 ccggtgatct ggagaagggt cggagtcccg ccaccgacga accagtagag gctgtagtaa
     7441 ccggggtttg ccgagacctg cggtacgccg gagaagggcg cagggatcga gagcggtacc
     7501 aggacgtcga ttccgatatc gagttgagcc gcggtttcag gcggcatcca gtccggcagc
     7561 gtttgatagg caactgcctg ggtggtgatc cgcggttccg gcgccgaacg taccgcgccc
     7621 ggtcgcgcgg ccgccagcac gacggaaagg agcatcaccg cgactagcaa ccaggcggtg
     7681 atcgatcgtt tcagcatgcg gtccgttccg aacgactgtg accaacgaga gccgaatggc
     7741 cgtcagtcag cgtagcatct cggtggccga tacggcgaga cgagaccgca gagttcgttt
     7801 cctatacttc gtgcgcgccg cgggttcgac cagttcggcc gtacgtcacg accgatcgca
     7861 gcaggggttc aatgctcgtc cggatcattg cctacagtat tgggagtatc gtcgcgatcc
     7921 tggcggtcgg cacgattttt acgtcggacc ttgtcgatta cgtcgacgag cggtcggtct
     7981 ttattttcgg gattgtgctc ggactgatga ccgcgttgct caagccgatc ctgagcgcgc
     8041 tgacacttcc catttcatgc ctgaccttcg gactgttcgc acttgtcctg aatgccgcga
     8101 tgttcggcgc cgcggcatgg ctgacacctg atgttgaggt gacggcactg ggggcgctcg
     8161 tcggcggcat cttcgtcgcc gtcatcaatg gggtcctcta ctcagtggtc gatcagtcgt
     8221 gaggtccgcg tcaaacgtgt tcgttgagat cggaacgatt caggtcaagt taccggcgcg
     8281 ctgccggttg gcgctgatcg ccgataccca tatctacccc tcgggccagc gctcaattcc
     8341 cgagccggtg ctggatctgt tcgatcgcgc cggcgtcgac cgcgtcatcc atgccggcga
     8401 tatcgcatgc gctccggtgc ttgaccagct cgcgacgatc gcgccggtca tcgccgtccg
     8461 tggtaacaac gatgacggcg actttgggcg cagcttgccg cacatggtcg agatgcaggt
     8521 cggacagcgg ctcgtgcggc tgatccatgg cgacggcggg cgatccgcgc gggctgtggc
     8581 aaccgacgtc gcacccggag cggactgcgt catctacggt cacagccaca ttccgaaggt
     8641 cgagcgcgcc ggcgacgcga tcctggtcaa tcccggctcg ccgaacgatc gccgctggca
     8701 cccacacttc ggcgtcgggt tcctcgaggt cacgccggac ctggtccggc ccgagctgat
     8761 tctgtttacc gacccgaccg accttgcccg ggtcagtcca acctggcgat cagacctgaa
     8821 gtgacggcgc ggggattggt gagacgctcg ggaccgtcgt ggtctcgata ccgaccgcat
     8881 gcaacagcgt gcccgcgatc ccgtccggcg tgactgcttt cagcccgacc gaatgctcgt
     8941 aggcggcgtc ggagatcgag cgcacgagcg gcaggttttc ccagagaagc acgttcggct
     9001 cgatcagtag gacgacggag ctgttcgtca tcaacgggaa ctggttgacg aggtactcga
     9061 gctgctcgcg ggattgaatc acctcgacat ggcggtgctc ccgggtaatc agcttcgcgt
     9121 agaggtagct gatcaggatc ttgagcacgg cggcgatcgg caccgccagg atcaggccga
     9181 gaatgccgcc gagcgcagtc ccgacgacca cacagaagat aaccagcagc gggtggaggt
     9241 gaacgaaccg gccgattacc gtcgggatca cgaacgcgtc ctccgcctgc cgcagcgcga
     9301 aataggccag cccgatgacg acggcgagcc cgacatggga ccaaccgaac ggcgtcgtgt
     9361 cctggaagat gctgaccgtg acggcgatcg cgccggcaat ccacggcccg acgagcggga
     9421 tcagctcgag gaagccggtc gcgatagcga tgatcagggc gtacttgacc ccgagcacgc
     9481 tcaacacggc gaacgacgcc gtcgacatga tcaaaacgag cagcgcctga ccgcgcagat
     9541 acgcgcccag tgttgcgttg atctcggcca ggaggcgatc gaactcggcg tggtacttgc
     9601 ggttaagaac agcccggaac gccatcacga atcggtcgcc gtggacgatg aagtagaagg
     9661 aggcaacgag gtagatcagg agctcgatgg caattgagaa ggtgctgaac aggaccggta
     9721 gcgccgcctt gccgagcgtg ttggcaatct ccgtcccgac ttcatcaagg cgctcgttga
     9781 cgtagctgct gtcgatcccc atctcgtcga ggcggccctg ctggttgtcg ttgatccagg
     9841 tttcgatgtc gtccgagaat ttggggatct catccttgaa ttgactgatc tgatcgacca
     9901 ggacggggcc aaagttgatt gcgaggatcg tcaggagcag gccgatcatg gcgtagagcc
     9961 agagcgtgat caacggcttg ggcagacgcg tgcgccggtg aatcatgctg acgaagggat
    10021 ggagcaagta ggcggtgatg acggcccagg cgaacggacg aagcgcgttc gccgcttcga
    10081 ggaggagcac tgctccggcg atcacgccga tccagatcac gatcgtccgc ccacgcggag
    10141 aaaggctcac ttccatcaat caactcgcct tgcgaggagt gggctggggc gtcacggcct
    10201 gatcgttcct gttgaattcc aagcgcgcag attctaacag agcgtggcac ggccatccgg
    10261 atgaatccgg cgaaccgctc cgtatactgt ggccggaacg atcatgcatc ttcccggagg
    10321 cccagttgga ttttgaccag ttcgagagcc tgaattttcg cctgcccggc ccgaccccgc
    10381 tgccgccgag tgtgcgggca gcgatggacc gcccggcgat tcaccaccgt ggaccgctcc
    10441 tcaaatcaac catgcggtcg atcctggcgc gactgaagga gatccaccgg accgagcagg
    10501 acttgctcgt ctggccgggt agcggatcgg cgggctggga aatcgcgatc accaacttgc
    10561 tctgcccggg agaccatgtg gtcgtcacgg tctgcggtga cttcggtgag cggttcgcaa
    10621 gtgtcgccgg caagcttggg ctggtcgtgc atcgcgtcga gaagccgtgg ggcgaggcga
    10681 tccggccgtc cgagttgaag ggcgtggtcg aatgcgtgcc cgagtgccag gcggtcctga
    10741 tcacgcacaa cgagacgtcg acgggcgtga ccaatccgtt gccggagctg gcggccgtgg
    10801 cccgggaggc cggcgcgctg accatcgtcg acgcggtcag ctcggccagc gccttgccgc
    10861 tagaggtgga ttcctgggat ctcgacttcg tcatttccgg atcgcagaag gcgtggatgt
    10921 gtcctcccgg tctggtgatc tgcgcgatcg ggaagcgagc gtggtcggca tatgacgcat
    10981 cgacgtaccc gcgcttcttc tgggatatca ccagtgcccg gaagtcggcc gccgagggca
    11041 tgacgccgac gactccgccg ctgcacctgc tcttcgcgct cgacgcagcc ctcgacctga
    11101 tggtcgacga aggggtcgag gcgatgtggg cacgtcatca cgcgctcggg gagtacgccc
    11161 gcggtcgggt tcgcgagctc ggactcgagt tgctggccga gccggacttc gcgtctgatt
    11221 cgctgacggc catccgcgtg ccggaagggc tctcggcccg ggcaatcatc gacgcgatgg
    11281 tcgcaaacca ccgggtgatg ctccaggccg ggcagggcgc catgaccgat caagtgctgc
    11341 gggttggtca catggggtgg gtgacggagc gggacctcga ggaagcgttc gacgcgctcg
    11401 gcgccacggt ggcgacgtcc gagtttcgcg ccagataaat tcggactttg cttattgtca
    11461 tgcaggggtc agcacgcgta tacttgctga ctgtgtcttt tcgcgacgcg agaatttggt
    11521 tgtccccgtc gagtgatcgg aggagccggg attcgagcgc attcaagtga caggttgcga
    11581 atctcttgat gactgagact gatgcgagtt tgatgagcgg gactgatgag tccactcaga
    11641 cgctctccct catggaagag tttctcaatg acccgagtct tgagtacaag tcgctcaagt
    11701 acggcgacgt catggacggg gtcatcatgc ggatcgaccg cgatgagatt ctcgtcgaca
    11761 tcggatcgaa gtctgagggg atcgtcccgt cgcgtgaatt ctccagcctg aacagcgagg
    11821 agaagggcga gctctccatc ggagataccg tcctcgtctt cgtcgtccaa cctgagaatc
    11881 aggagggcca ggcggtcgtc tcgatcgacc gggcgcggca ggagaagagc tggcgtcgac
    11941 tccaggagct ctacgaggcg aatgagtcga tccaggccga agttaccaat tacaacaagg
    12001 gcggtctcct ggtgaacctg gatggcgtgc gcggcttcgt gccggcgtcg caggtgaccg
    12061 agatccgcgg tggcgacgat gccagcaagc aggccgacat ggccaagctg atcggcacca
    12121 agctcaacct caaggtcatc gaaatcaacc ggcatcgcaa ccggctgatc ctctccgagc
    12181 gccaggccat gcaggagcgg cgcgatgaga tgaaggagaa gctgattgag gaactgaagg
    12241 agggcgaggt ccggcatggg cgcgtctcct ccatctgcga cttcggcgcc ttcgtcgaca
    12301 ttggcggtgc ggacggactc gttcacctct cggagctctc ctggagccga gtgcgccacc
    12361 caagcgaagt cctcaaagtc ggccaggaag tcgatgtggc ggtcctcggg atcaacccgc
    12421 aggagcgcaa gatcgcgctc tcgctcaagc ggacgcaggc tgagccatgg agccaggtcg
    12481 gcgacaagta cgaggtcggc cagctcgttc gcggcacgat cacgcagctt gcgaacttcg
    12541 gcgccttcgc tcgcatcgag gatggcatcg agggtctgat ccacgtctcg gagctgaccg
    12601 acgagcggat cacgcatccg aagcagatcg tcaaggaggg cgacgagctc atcctgcgca
    12661 tcatccgtat cgatccgcag cgccgccgca tggggctcag cctccgccgc gcgctcgata
    12721 cgccggatca ggagctgatg gc
//
LOCUS       DATAOZ010000049         5568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6862853, whole genome shotgun sequence.
ACCESSION   DATAOZ010000049 DATAOZ010000000
VERSION     DATAOZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6862853"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1400
                     /locus_tag="VHR64_01460"
     CDS             336..1400
                     /locus_tag="VHR64_01460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3301515.1"
                     /translation="MNRRDLIAGLAATTVGARVVRPVAAQVIPWPTRDWAVVDPGDVG
                     MSADQLTAADQQIIAAYPDITGVVVVRGGRIGFERYYGSDYGQDDPVKIRSITKSVTG
                     TLIGMAIDDGLLALDSTLGELIPNVIPAGADPLTPTITVENLLTMTSGWDWDIHADYP
                     TLIAADNYSQLTLSLPVAYTPGSFYAYNTGGSHLLSVIVEAVTGERTIQYADRRLFGP
                     IGINRPRWQRSPEGPACGGFGLELTARDLARFGLLALRSGEWDGQQLVSADWFAAATT
                     YQSSGDSTGYAAYGYQWWVIPESPYGAYFGLGFGSNYLYVAPALDLIVVVLKGFETPP
                     NPVSIVRPLIEGHFLPAVTT"
     gene            complement(1487..2260)
                     /locus_tag="VHR64_01465"
     CDS             complement(1487..2260)
                     /locus_tag="VHR64_01465"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX3301516.1"
                     /translation="MSGLFDLTGKVAVVTGAGAGLGANFSRALAEAGASVVCADINTE
                     HAERTAAKLTGDGYKAVAMHADVTDEANIEQMIQRAVDEFGSVDILVNNAGIAAGIGD
                     PETIPLEDWKRTVDVNLTGVFACAQAAARAMIKAETGGSIINLASILGFAASEPVAAS
                     AYAATKGAVVNLTRDLAVHWAQHNIRVNALGPAYFPSDMTGGIFEDKSLLSEIERRTP
                     MGRVGRPNELDGPIVFLASDASSYITGQTIYVDGGWTAW"
     gene            2525..3460
                     /locus_tag="VHR64_01470"
     CDS             2525..3460
                     /locus_tag="VHR64_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3301517.1"
                     /translation="MALQLITRRSLLIGSAATGIAALSGIGTTLDAEAVGLAGVIGSV
                     SSASGLNLRYGPNSAQRVEKWLPNGTPLTIRGTSGDWFKVTALGLTGWVNSWYVVLKG
                     TRSTTIQRGNPALKRVALTFDCGSDLGYTNQILATLANHGIPASFGMTGSWMSANPDS
                     AKKIASGGYQFINHTLNHPSYTGASTPGAGPSSPAKRLAQIQANENAIRTVTGMIAKP
                     YWRPPYGDIDSGVLRDVGALGYSKTAMWTIDSLGWNGLTADEIYSRVMSKMVWGAIVL
                     MHVGAASQDANALDRIIRDLKAQHYTFATVAVVTA"
     gene            complement(3461..4009)
                     /locus_tag="VHR64_01475"
     CDS             complement(3461..4009)
                     /locus_tag="VHR64_01475"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX3301518.1"
                     /translation="MRRLYTRRGDLGETDLLGERVAKDDPRIDLIGELDETTSVLGLA
                     RSLSISQRGNQILVDIQRDLYRVMADLAFVTDTRPPGYETSAEWVERIETLTDQLTAE
                     VELPRQFVLPGETQASAALDVARAVVRRAERQAVHLYRTKVITNEHIVGYLNRLSSLL
                     FVLARFVEAEQGAHAEQAKQST"
     gene            4067..5185
                     /locus_tag="VHR64_01480"
     CDS             4067..5185
                     /locus_tag="VHR64_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3301519.1"
                     /translation="MVLTQRFPRSPEAIRVAVVGTGFGEAVHLPALQQVPEFRIMAVC
                     SRRAERAHSAAQAFGATIATTDYRELVERSDVDAVIIATPPHLHHSMTMTALGAGKHV
                     LCEKPMARTVAEAKDMVKMAERSNVIAMVNHEFRFIPARAYAKELIESGFIGEPYSAS
                     MSFYRSSLNDPRGVPFTWLMEQDKAGGMLGAIGSHHIDSLRWWMGEIRAASGALATMV
                     KKRRAGDSTQMLRVDADDNFALVLQFENGAIGTIHYSATAAHEPSDLIVVSGAEGTLV
                     LTSDGRLQAGRRGELIADTLIPDQYIRGASSGPHPLIHPTVYLLKQWAAAIRSGTAQW
                     PSFEDGYKVQEVIDAIGRSVQTRRSVELQRGRRGAAVT"
     gene            5272..>5568
                     /locus_tag="VHR64_01485"
     CDS             5272..>5568
                     /locus_tag="VHR64_01485"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX3301520.1"
                     /translation="MSMTMPAPAQPILSPDVVLDTPSMLVDETLMEQNISEMQALANS
                     FGAQLRPHIKTHKTPQIALRQISHGARGITCAKLGEAEVMVEAGVEDVLMAYPVV"
BASE COUNT          973 a         1781 c         1797 g         1017 t
ORIGIN      
        1 agtcgagccc gacatcctcg gccactttcg ccatctcggc caattgcggc cacgtgtact
       61 cgtactcgac ttcggggagc tgcaccccaa ccttcaacgg ccgcgccatg gcgttccttc
      121 cttgatagcg actccgatag accggcagtg tactggtcgg gttgagggtg gagggttgag
      181 ggttgaggga tcacgaacgt agcgccgact ccccgtggtc ggctggggtg acagcacgtg
      241 cagaaatgga gccaactacc agcatcggtt aggtcacccg acagcgggcg gcccagaggc
      301 gggccgcgct acgattctgc gcggaggtgc gccgtatgaa tcgacgggat ctgattgccg
      361 gtctggctgc cactactgtg ggtgcgcgcg tcgtgcgtcc ggtggcggcg caggtgattc
      421 cctggccgac gcgggattgg gcggtggtcg atcccggtga tgtcggtatg agcgcggatc
      481 agcttaccgc cgctgaccag cagatcatcg ccgcctaccc ggatatcacc ggggtggtgg
      541 tcgtgcgcgg cggtcggatc ggcttcgagc gctattacgg ctccgactat gggcaggacg
      601 acccggtcaa aattcgctcg attaccaaga gcgtcacggg gacgctgatc ggcatggcca
      661 tcgacgacgg cctgctcgcg ctcgacagca cgctcggcga gctgatcccg aatgtgatcc
      721 cggccggcgc cgaccccctg acgccgacga tcacagttga gaacctgctg acgatgacct
      781 ccggttggga ctgggacatc cacgccgatt atccgaccct catcgccgcc gacaactaca
      841 gccagctcac gctcagcctg ccggtcgcct atacgccggg cagcttctac gcatacaaca
      901 ccggcggctc gcacctgctg tcggtgatcg tcgaggcggt gaccggcgag cgcactatcc
      961 agtacgcgga ccggcgattg ttcggcccga tcggcatcaa ccggccgcgc tggcaacgat
     1021 cgcccgaggg tccggcctgc ggcggcttcg gtctcgaatt gaccgcgcgc gatttggccc
     1081 ggttcggcct gctcgcgttg cgttcgggcg aatgggacgg ccagcagttg gtctcggctg
     1141 actggtttgc cgcggcaacc acgtatcaat cgagtggcga ctcaaccggg tatgcggcct
     1201 acggctatca gtggtgggtc attccggaga gcccgtacgg cgcgtatttc ggtctcggct
     1261 tcggcagcaa ttacctctat gtcgcgcccg cgctggacct gatcgtggtc gtgctcaagg
     1321 gattcgagac gccgcccaac ccggtctcga tcgtccgtcc cctgatcgag ggccatttcc
     1381 tgccggccgt gacgacgtga cagcaaacgg agttccgggc ggctcacccg gaactccgtc
     1441 gaacaaggga cgcgaccacg ggacatgtcc atgctcggcg ggttcgctac caggcggtcc
     1501 agccgccgtc gacgtagatc gtttggccgg tgatgtagga cgatgcgtcg gaggcaagga
     1561 agacgatcgg gccgtccagt tcgttgggcc gaccgaccct gcccatggga gtccgccgct
     1621 cgatctcgga aagcaggctt ttgtcctcga agatcccgcc cgtcatgtcc gacgggaagt
     1681 aggcggggcc gagcgcattc acccggatgt tgtgctgtgc ccagtgcacc gccaaatcgc
     1741 gcgtcaggtt gacgaccgca cccttggtcg ccgcgtaggc acttgccgca acgggctccg
     1801 aggccgcgaa gcccagaatc gaggcgaggt tgatgatcga gccgccggtt tcggccttga
     1861 tcatcgcccg ggccgcggct tgggcacagg caaagacgcc ggtcaggttg acgtccacgg
     1921 tccgcttcca atcctcgagt ggaatcgttt ccggatcgcc aatccctgcc gcgatgccgg
     1981 cattgttgac gaggatgtcg acactgccga attcatcgac ggcccgctgg atcatctgct
     2041 cgatattggc ctcatctgtc acgtctgcat gcattgccac cgccttgtaa ccgtccccgg
     2101 tcaactttgc ggccgtgcgc tcggcgtgct cggtgttgat atcggcacag acgacagagg
     2161 caccagcttc agccagcgct cgcgagaagt tggcgccgag accggcgccc gctccggtca
     2221 cgaccgcaac cttgcctgtc aagtcgaaca atccgctcat gacgggtcac tccttctgtc
     2281 aatgcccact ctcgggtatc gggagcccga gcctctatcc agtgttgtat tgatggcaac
     2341 caattggcat gacgagaggg ccgcgaatat gtcagttgtg tgacgcctta gctctcgttg
     2401 caggtttgac actcggaagc gaccgctttt gcggagcttc ttcgttctgg tgagaagatg
     2461 tgatcaatgg gagcgggtgc tgtgtcgatg tcggtttgac cccgcgccct gaaggagcct
     2521 gattatggcg ctgcaactca tcacgcggcg tagcctcctg atcggcagcg cggcaacagg
     2581 cattgccgcg ctttccggga tcgggacgac cctcgatgcg gaggccgtcg ggctggcggg
     2641 cgtgatcggc tccgtttcgt cggcatcggg gctgaatctc agatatggtc cgaattcggc
     2701 ccagcgggtc gagaaatggt tgccgaacgg cacgccactg actatccgcg gtacctccgg
     2761 cgattggttc aaggttacgg cgcttgggct caccggatgg gtcaattcct ggtatgtcgt
     2821 gttgaagggc acgagatcga ccacgattca gcgcggtaat ccggctctca agcgagtggc
     2881 gctcaccttc gattgcggct cggatctcgg ctacaccaac cagatcctag cgacccttgc
     2941 caatcacggt attccggcca gctttggcat gaccggatcc tggatgtcgg cgaatcctga
     3001 cagcgcgaag aagatcgcca gtggcggata tcagttcatc aaccataccc tcaaccaccc
     3061 ctcatacacc ggggcttcaa cgcctggcgc cggcccgtcc agcccggcca agcgactcgc
     3121 ccaaatccag gccaatgaga acgccattcg caccgtgacc ggcatgattg ccaagccata
     3181 ttggcggccg ccctacgggg acatcgattc cggcgtgctg cgtgatgtcg gcgcactcgg
     3241 ctattcgaaa accgcgatgt ggacgatcga ctcgctcggc tggaatggcc tgactgcgga
     3301 cgagatctac tcgcgggtga tgagcaagat ggtttggggt gcgatcgtcc tgatgcacgt
     3361 cggagccgcg tcgcaggacg cgaacgcact cgaccggatc atccgcgacc tcaaggcgca
     3421 acactacaca ttcgcgacgg tcgcggtggt gacggcgtga tcaggtcgat tgcttggctt
     3481 gctcggcgtg ggcgccctgc tcggcctcga cgaagcgagc cagcacgaag agcagcgacg
     3541 acagccggtt caaatacccg acgatgtgct cgttcgtaat gaccttcgtc cgatagaggt
     3601 ggactgcctg ccgctcggct cggcgcacga ccgcacgggc gacatcgagc gcggcgctcg
     3661 cctgcgtctc gcccggcagc acgaactgcc gcggcagctc gacctcggcg gtcaactggt
     3721 cggttagcgt ctcgatccgc tcgacccact cggcgctggt ctcgtagccg ggcgggcgcg
     3781 tatctgtcac gaaggcgagg tcggccatga ctcggtagag atcgcgctga atgtcgacca
     3841 ggatctggtt gccccgctgc gatatcgaca gggatcgggc gagcccaagg accgaggtcg
     3901 tctcgtccag ctctccgatc aggtcgatgc gcgggtcatc cttggcgacg cgctcgccga
     3961 ggaggtccgt ttcgccgagg tcgccccggc gggtatagag tcgacgcatg ggctaatcct
     4021 cctgccgttg aacgatgcca tgccggatgt ctggtagcat cacgccatgg tactgacgca
     4081 acgattccca cgatcacccg aggcaattcg cgtcgcagtc gttggcaccg ggttcggtga
     4141 ggcagttcat ctgccggcgc tccagcaagt gccggagttc cggatcatgg ccgtctgctc
     4201 gcgccgggcc gagcgggccc acagtgccgc gcaggccttc ggcgcgacga tcgccaccac
     4261 agattaccgc gaactggtcg agcgatccga cgtcgacgcg gtgatcattg cgacgccacc
     4321 gcacctccac cactcgatga cgatgaccgc gctcggcgcc ggcaagcacg tgctgtgcga
     4381 gaagccgatg gcccggacgg ttgccgaggc caaggatatg gtcaagatgg ccgagcgctc
     4441 gaacgtgatc gcgatggtca accacgagtt tcgcttcatt ccggcccggg cctatgccaa
     4501 ggagctgatc gagtccgggt tcatcggcga gccctactcc gcctcgatga gtttctaccg
     4561 ctcgagcctc aacgatcccc gcggcgtgcc cttcacgtgg ctgatggagc aggacaaggc
     4621 cggcgggatg ctcggcgcga tcggctcgca tcacatcgat tccctgcgct ggtggatggg
     4681 cgaaatccgg gcggcgagcg gcgcgctggc cacaatggtc aagaagcggc gcgcgggcga
     4741 ctccacccag atgctgcggg tcgatgccga cgacaatttc gcgctcgtgc tccagttcga
     4801 gaatggcgcg atcggcacga tccactactc ggccacggcc gcgcatgagc cctccgacct
     4861 gatcgtggtc tccggcgctg agggcaccct cgttctcacc tcggacggcc ggctgcaggc
     4921 gggccggcgc ggcgaactga ttgccgatac gctgatcccg gaccagtaca ttcgcggcgc
     4981 gtcatcgggt ccgcacccgc tgatccatcc gaccgtctac ctgctcaagc aatgggcggc
     5041 ggcgatccgc agcggcacgg cgcagtggcc ctccttcgag gacgggtaca aggtgcagga
     5101 ggtcatcgac gcgatcggcc gctccgtgca gacacggcga tcggtcgagc tgcagcgcgg
     5161 ccggcgcgga gcggcggtga cctgaaaacc ggcacattcg cgggtgtgtg atttgagata
     5221 ctgtgcgcac tacggcagca acgcagcgcc aaaacgcacg gagagcacac catgagcatg
     5281 acgatgccag cacccgccca acccatcctc tcgccggacg ttgtgctgga taccccctcg
     5341 atgcttgtcg acgagacgct gatggagcag aacatcagcg agatgcaggc gctggccaac
     5401 agcttcggcg cgcagcttcg cccgcacatc aagacccata agacgccgca gattgcgctt
     5461 cgccagatct cgcacggtgc gcgtggcatc acttgcgcca agctcggaga ggccgaagtg
     5521 atggtcgagg ccggggtcga ggatgtgctg atggcctatc cggtcgtg
//
LOCUS       DATAOZ010000050         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6863755, whole genome shotgun sequence.
ACCESSION   DATAOZ010000050 DATAOZ010000000
VERSION     DATAOZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6863755"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1633)
                     /gene="murF"
                     /locus_tag="VHR64_01490"
     CDS             complement(212..1633)
                     /gene="murF"
                     /locus_tag="VHR64_01490"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX3301521.1"
                     /translation="MPPPVMLSEVMVAAGARLVGELAHDTAFRWIERDSRVLQPGDLF
                     IAVRGELFDGHDFVADAAAKGAAAALVSIDWAADNAANAPVPLLVVPDPVLALQEVAR
                     ARRKQRDVTVVGITGSIGKTSTKEVVAAVVGQKWTTYRSPGNLNSEIGLPLSLLEMQD
                     DVEAAVFEMGGAYAFGELALLASIALPKIGVVTNVHPVHLERMGTIDNIARTKAELVE
                     SLPPDGVAVLNGDDARVLAMAELAPGGVITYGLSPTNDVWASDVESEGLRGSTFKLHL
                     AGETHHVRVPLVGGHAPYLALPAIAVGHALGMHISEMLPGFDDPEIQVRLLVLDGPNG
                     SMLIDDTYNASAPSVLSALGLLDEIAPGRRIAVLGDMRELGEESETQHRVVGRRAAEV
                     VDELITFGPLAQLIADAAVLAQQEEVEARVERVRSFAIDERDALIAYLRQESAEGDVV
                     LLKGSRGLEMERIVAALQPSGED"
     gene            complement(1645..2607)
                     /gene="rsmH"
                     /locus_tag="VHR64_01495"
     CDS             complement(1645..2607)
                     /gene="rsmH"
                     /locus_tag="VHR64_01495"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX3301522.1"
                     /translation="MISELDVIAMEPTHRPVLLTEAVDQLVWRPGGVYVDGTFGGGGH
                     TREILSRDAGNRVIAFDLDLSAIDRGRNLQQDVGPDRLTIIHDNFATMASHLDQLGID
                     HVDGVLLDLGMSSFQLDDAERGFAFRFDGPLDMRFDQAAGPTAAELIATTDEAELADI
                     IWRYGEERRSRQIARALVAERDRQPIETTGQLAGLVARIVGRGKGGTHPATRTFQALR
                     IAVNREFDVLERALESAVERMAPAGRLVVIAFHSLEDRIVKQFIVRESATCVCPPEQV
                     ICTCETTPRLRRLGSSRKPSAEEQSGNPRSRSAVMRVAERLVES"
BASE COUNT          522 a          935 c          854 g          492 t
ORIGIN      
        1 cggcgttcct gttttccaga gttgcgtctt cggcccgtcg atgcggacct gtttgccgtt
       61 gttcttgagc cgcaggtagg tgacgatcgg ccggccgacg atcagcgtca gcataaaagc
      121 gagaccggcc aacccgagcg acacggccat gttctcgtca ggctcggtcg gcatgattga
      181 gctggtcaga atcggaagga acaaagtcaa ttcagtcttc acccgatggc tgcagggcgg
      241 ccacgattcg ctccatttcg agcccgcggg atcccttcag aaggacaacg tcgccttccg
      301 ccgactcctg ccgcaaatac gcgatcaggg catcacgttc atcgattgcg aacgaacgca
      361 cacgctccac acgcgcttcg acctcttctt gttgagcaag gacagccgcg tccgcgatca
      421 actgggcgag cggcccgaag gtgatcaatt cgtcgaccac ttcggcggca cgtcgcccga
      481 caacccggtg ctgggtttcg ctctcttcgc caagctcgcg catatcccca agcacggcga
      541 tgcgccgccc gggagcgatc tcatcgagca ggcccaatgc ggacagcacc gacggggcgc
      601 tcgcattata ggtgtcatcg atcaacatcg acccgttcgg tccgtccagc accagcaggc
      661 gaacctggat ctccgggtcg tcgaatcctg gcagcatctc cgagatgtgc atgccgagcg
      721 catgcccgac cgcgatcgcc ggtagcgcaa ggtacggcgc atgaccgccg actagcggca
      781 cgcggacgtg gtgtgtctct cccgccaggt ggagcttgaa ggtcgagccg cgtaatccct
      841 cggactcgac atccgatgcc cagacatcgt tggtcggcga caggccgtag gtaatgacgc
      901 ccccaggcgc aagctccgcc attgccagga cgcgcgcgtc gtcgccattc agaactgcga
      961 cgccatccgg aggcagcgat tcgaccagtt cggccttggt ccgggcgatg ttatcgatcg
     1021 tgcccatccg ctcgaggtgc accgggtgca cgttggtgac cacgccgatc ttcggcagcg
     1081 caatcgaggc caacagggcc agctctccga aggcgtatgc gccgcccatc tcgaacacgg
     1141 ccgcttcgac atcgtcctgc atttcgagca gagagagtgg caggccaatc tcgctgttga
     1201 gattgccggg acttcgatac gtcgtccact tctgacccac gaccgcggcg acgacctctt
     1261 tggtgctcgt cttaccgatg ctgccggtga taccaaccac cgtcacatcg cgctgcttgc
     1321 ggcgagcacg tgcgacctcc tggagggcca gaaccggatc ggggacgacc agtaacggca
     1381 cgggcgcgtt ggctgcgttg tcggccgccc agtcgatcga gaccagtgcc gcagcggcgc
     1441 ccttcgcggc tgcatcagcg acgaagtcgt gtccgtcgaa aagctcgccg cgcacggcaa
     1501 tgaagagatc gcccggctgc aggacgcgcg aatcccgctc gatccagcga aatgcggtgt
     1561 catgcgccag ctcgccgacc aaacgagccc cagcggccac cattacttcg ctcagcatga
     1621 ccggtggcgg catcgattct cccctcatga ttcgaccagc cgctccgcga cccgcatcac
     1681 ggccgatcga cttcgcgggt tgccggattg ttcctcggcg gacggcttcc ggctcgatcc
     1741 cagccggcgc aggcgcggtg tggtttcgca tgtgcaaata acctgttcgg gtgggcaaac
     1801 gcaggtcgcg gattcccgaa cgatgaactg cttgacgatc cggtcctcga gcgaatgaaa
     1861 ggcgatcacg acgagccggc cagcgggcgc catccgctcg acagccgatt ccagcgcccg
     1921 ctcgagaaca tcgaactcgc gattgaccgc gattcgcaac gcctgaaacg tccgggtcgc
     1981 ggggtgcgtt ccgcccttgc cgcggccgac gatccgggcc acgagcccgg cgagttgtcc
     2041 cgtcgtctcg atcggctgcc gatcgcgctc ggccaccagt gcccgcgcga tctggcggga
     2101 gcgccgctcc tcgccgtacc gccagatgat gtcggccagt tccgcttcgt cggttgtcgc
     2161 gatcaactca gcggcagttg ggccggccgc ctggtcgaac cgcatgtcga gcggaccgtc
     2221 gaagcggaat gcgaagccgc gctcagcatc atcgagctga aatgaggaca tgccgagatc
     2281 gagcagcacg ccatcgacgt gatcgatacc gagctgatcg agatggctcg ccatggtcgc
     2341 gaaattgtcg tggatgatgg tcagccggtc cgggccgacg tcctgctgaa ggtttcggcc
     2401 gcgatcgatc gcgctgaggt cgaggtcgaa tgcgatgacc cgattcccgg cgtcgcggct
     2461 cagaatctcc cgcgtgtggc ccccgccacc aaacgttcca tcgacgtaga cgccgcccgg
     2521 tcgccagaca agctgatcga cggcttccgt cagcagtacg gggcggtgag ttggctccat
     2581 ggcaatcacg tcgagctcag aaatcaaatc atggacccga gacgaccaat gatctcatct
     2641 gcctgcgcat cgagccgctg gtggatcgtg tcccaggcat cgactgccca gagttcgatg
     2701 accgaatcca ttcctacgac gaccacgtta cgatcgatcc cggcaaatcg acgcagctcc
     2761 acggggagca gaatacgccc ctgaccatcg ggctcaagcg tca
//
LOCUS       DATAOZ010000051         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2655480, whole genome shotgun sequence.
ACCESSION   DATAOZ010000051 DATAOZ010000000
VERSION     DATAOZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655480"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1227)
                     /locus_tag="VHR64_01500"
     CDS             complement(145..1227)
                     /locus_tag="VHR64_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX3301523.1"
                     /translation="MPIVLRARLLAFVSRFGPLLWLTLLIGFAAFFIDRHGGELRKAA
                     GLIVRGDPRWVLAMIATQLMSLALVTGKWRMLLARVGAQLPAQTVLGAYLRRHLISTV
                     LPIGGPAGLVHFMRDLGKHRVSSGTILHASMLASLVNQAALALYLITALGWLAIAGRA
                     TGTMLIAAILMTALVLGCTAGFSFILKRGELPQVLSKRLPAKVANSVADIRANGFHWR
                     DIAMALPFAIMVNIASIALLAGALMVVGQHPALTIILSARVAATISGWMLPVMQGGGA
                     VELSIVGALHAGGVPLPEAVAAMVLFRIVQVWIPIALGGIAMVPWDRRDEFSARLAPA
                     RLSLATGLLVAVLPVGAAVAAYYTLL"
     gene            complement(1425..2420)
                     /locus_tag="VHR64_01505"
     CDS             complement(1425..2420)
                     /locus_tag="VHR64_01505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX3301524.1"
                     /translation="MSTPRIDPDPLSPLNQMQRYLVHEFIDDYQDGLLSRRDLTAKIG
                     YIAGGAAAAAAILTRFGLDVGTAEAQEASATPQATGPQSPISVPPDDPAVFGVDITFP
                     SGGGDYTAYEARPATAATPAEGTPAANGSGLILVCHENRGLTDHIRDVARRLAKVGYV
                     ACAVDLLSPKGGTAANNPDAIPGMLTSGDINRHVQAFMDAITFYQQEGEAFSERVGMV
                     GFCFGGGITWRTVTQEARLKAAVPYYGPPPPLDQVPNIKAAVLGVYSDDPGDFANEGR
                     DDLEAALRAAGIAYQFKVYPGTQHAFNNDTGPRYNEQQAIAAWNDTLAWFAQYLV"
     gene            complement(2655..>2901)
                     /locus_tag="VHR64_01510"
     CDS             complement(2655..>2901)
                     /locus_tag="VHR64_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3301525.1"
                     /translation="AVRVKAEGRVDLRGFFGVSDVPAGFQDVAFTVEVDSPASPEVIA
                     ELVAEVNARCPVLDILRSPVPVTGAYLHNGQPVASGS"
BASE COUNT          546 a          828 c         1068 g          459 t
ORIGIN      
        1 cgaagttggg ttgatggtgg atggtgattg gtagatggaa cggaaggggt ggtcagcagt
       61 caatgaccgg tagccagtgc gatcgttccc gaaaaccaaa aaccgaaaac cgcgagacca
      121 aagacgagag acgaaagacc cccgttagag gagcgtgtaa taggcggcta cggcggcgcc
      181 aaccggcagg acggcgacga ggaggccggt ggccaggctg agacgggccg gggcgagacg
      241 ggcagagaac tcatcgcgcc ggtcccacgg caccatcgcg atgccgccga gggcgatcgg
      301 gatccagacc tgcacgatcc ggaagaggac catggcggcc accgcttccg gcagcggaac
      361 gccgccggcg tgaagcgcgc cgactatgga gagctcgacc gcgccgccgc cctgcatgac
      421 cggcagcatc cagccggaga tcgtcgccgc gacgcgcgcg gaaaggatga tcgtgagcgc
      481 ggggtgctgg ccgacaacca tcagcgcgcc ggcgaggagg gcgatgctgg cgatgttgac
      541 catgatcgcg aagggcagcg ccatagcgat gtcgcgccag tggaacccgt tggcgcggat
      601 gtctgcaacc gagtttgcga cctttgccgg gaggcgcttg ctcagcacct gggggagctc
      661 gccacgcttg aggatgaagc tgaatccggc tgtgcagccg aggaccagtg cagtcatgag
      721 gatggcggca atcagcatcg taccggtggc ccggccggcg attgccagcc agccaagcgc
      781 cgtgatcagg taaagcgcga gggctgcctg gttgaccagc gaagccagca tcgacgcgtg
      841 caggatcgtg ccgctgctga cgcgatgctt gccgaggtcc cgcatgaagt ggaccaggcc
      901 ggcgggtccg ccgattggca ggaccgtgct gatcaggtga cggcgcaggt aggcgccgag
      961 gacggtctgg gcggggagtt gggcgccgac gcgggcgagc agcatgcgcc atttgccggt
     1021 gaccagggcc agcgacatca actgggttgc gatcatcgcg aggacccagc gggggtcgcc
     1081 gcggacgatc aatccggccg ccttacggag ctcgccgccg tgccgatcga tgaagaatgc
     1141 cgcgaaaccg atgagtaggg taagccaaag gagagggcca aatcggctta cgaacgcgag
     1201 gaggcgagca cgaaggacga tgggcaaggg ttgaatcctt tgctgggcgt ggaaagttcc
     1261 tccccgggaa ccggggtgtt acgctcccag cctcacgcgt aacggtgaat cattaatcaa
     1321 gccaagaata gcgtgcaatg cccggactac gaacgtgcag gagtgcacgt tccgggtagc
     1381 tccctgggcc ggaagctacc cggacgtctg acctattgcc gggctcaaac caggtattgg
     1441 gcaaaccagg cgagggtgtc gttccaggct gcgatcgcct gctgctcgtt gtagcgcggg
     1501 ccggtgtcgt tgttgaaggc gtgctgggtg cctggataga ccttgaactg gtaggcaatg
     1561 ccggcggccc tgagggctgc ctcgaggtca tctcggccct cattggcgaa gtcgcccgga
     1621 tcgtccgaat agacgccgag aacggcggct ttgatattcg gaacctgatc aagtggcggg
     1681 ggcggtccgt aatagggtac agcggccttg agccgggctt cctgcgtgac ggtacgccag
     1741 gtgatgccgc cgccgaagca gaaaccgacc atcccgacgc gttcggagaa ggcctcgcct
     1801 tcctgctggt aaaaggtgat cgcatccatg aaggcctgga catggcggtt gatatcgcct
     1861 gaggtcagca tgccagggat agcatccgga ttgttggcgg cggtaccgcc tttggggctg
     1921 agaagatcga ctgcacaggc gacatagccc accttggcca ggcggcgggc gacgtcgcgg
     1981 atgtggtcgg tcaggccgcg gttttcgtgg cagacgagga ttaggccgga gccgttggcg
     2041 gccggcgttc cttcggcggg ggttgcggct gtggccgggc gggcctcgta ggcggtgtag
     2101 tcgccgccac cgctcgggaa cgtgatatcg acgccgaaga ccgcgggatc gtcgggcgga
     2161 acggagatcg gactttgggg accggtggcc tggggagtgg cgctggcctc ctgcgcctcg
     2221 gccgtgccga cgtcgaggcc gaagcgggtc aggatcgcgg ccgcggccgc cgctccaccg
     2281 gcgatgtacc cgattttggc ggtcaggtcg cgccgggaga gcaggccatc ctggtagtca
     2341 tcgatgaact cgtggaccag gtagcgctgc atctggttga ggggactcaa cggatcgggg
     2401 tcaattcgcg gcgtggacat gcgtcgctcc ttgcgtgcgc cggtgggcag ccgcatctgc
     2461 ctggcgcgct gatcgcaagc ccgctgcgcg cgcagcacct cttcccgcat tgcaccttat
     2521 cggcggatgc tgggagtttg ctgggagacg gtgtggcgga gactacggtt gagagttgag
     2581 ggttgaggga acgagaaagg ttagcagtga ccggtacctg gtagccgata cgctcgttcc
     2641 caataaccga aaacctagga gccggatgcg accggttgac cgttgtggag gtaggcaccg
     2701 gtgaccggga ccgggctgcg cagaatgtcg aggacggggc agcgggcgtt aacctcggcc
     2761 accagctcgg cgatgacttc gggtgaggcc gggctgtcga cctcgaccgt gaaggcgaca
     2821 tcctggaaac cggccgggac atccgagact ccgaagaagc cgcgcagatc gacccggccc
     2881 tcggccttga cccggaccgc a
//
LOCUS       DATAOZ010000052        10198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7126749, whole genome shotgun sequence.
ACCESSION   DATAOZ010000052 DATAOZ010000000
VERSION     DATAOZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7126749"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2206)
                     /locus_tag="VHR64_01515"
     CDS             complement(<1..2206)
                     /locus_tag="VHR64_01515"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HEX3301526.1"
                     /translation="MTAVDTDQISETDRETSDFFTYSGSRLRHVAMPLGGVGAGHIAL
                     GGDGGLRQWQIHNQVNHRGFVPDSFFAIRVTSTEPPLNVVRLLQSREVAALTPDPTPL
                     VNDDHIPEDQRRLVELFPGVQRTEFSALYPFARVRYLDRELPLEVSLEAHTPFVPLNE
                     VASGIPAILFSFTLRNAGHLNLQGALAATLQNAVGWDGLTPISGNRNPLYGGNTNRVR
                     RSPDRTSLVMENLSLPHDHPGAGQMMLTALANNVRVYEQWTTPQQFLRFMEGLNASRQ
                     FVGQDSPTQRAYRNLPILQHGPSPAGTTWNGGLMVPFHLGPGEEIQITFFLSWFFPNR
                     YVNFDQFGRMRDYGHSRFWLGNAYATRYADAIDVTDHLVRERAELERVSRRWEETLRG
                     SSLPDWLVEAMAAQGSLIRSPTTFWTEDGNFFGFEGSLGASTSMWNGDFGGSCPLNCT
                     HVWNYEMALSRLFPRLEQRMRTTEFEHVQAPEGYIPHRTILPLYLKQLWNEPIGGPRQ
                     PALDGMLGAVLKTYREVRQGGDVPWLRDHWENVRRLLEYIRRTWDENGDGVLEGEQGN
                     TYDIHFFGPNIYIGGLWLAALRAGEELAKIVGEEAYAADLHELFTAGSARYDELLWNG
                     EYYIQLLDNNTPLEDQFGDGCLSDQLFGQWWAHMLGLGYILPQEHVRTTLRSIVRYNT
                     REGFGDFEHGFRVYADRDDNGLLICTWPKGGRPTVPVRYCDEVWTGIEYQVAA"
     gene            2402..3745
                     /locus_tag="VHR64_01520"
     CDS             2402..3745
                     /locus_tag="VHR64_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3301527.1"
                     /translation="MDEKTRTSLSRRSLLKGAAATGIAGGAVLAGARSSTFAAPALRI
                     SATRPGYQVSGKVTLAYLGTADQAAVWNNLFALFQEKYPDIELEAQANPVNDWAAFFD
                     AISTQIAGGKVPDVVQVATEGQRLFASRGLCEPINDLIDRDKAELADYFEDIHPKLIE
                     WDQTYNTTKDGSRYYLPGDFNTMGYWYNSEVFTTNGIDEPTDDWTWDQVLAAGEALKA
                     KGIYAINVTPEYFISVMPWLLTNGASTLSADWTTATVNTPEAIEAATFMRTLVEKEYS
                     PLPGGTFDQFTATAQGKMAAFGGGRWPIINMRNLKVVDKMKIVHWPQKKQKGSPVGWG
                     AYPIMKGTKNKEAAWTFLKFLASKEASVYFAEQGGTIVPGRKSVANSDSYLANAPEGS
                     LKLYDALEYATPIPSPDKGNVVQSAIQDAFLQVLTGAVEPEEGLNALNDEIQSNL"
     gene            3771..4730
                     /locus_tag="VHR64_01525"
     CDS             3771..4730
                     /locus_tag="VHR64_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3301528.1"
                     /translation="MAAQADAGVAISPGIPSMSPTRRAGRIGLARREMLIGYAFLSPA
                     LILFLVFIAGPLLGAIGLSFYQWDLFSDRTFIGLQNYRALRDDEVARTAIRNSFYFAF
                     WSIVLHISIGLLLALAVNRAINGVLRYFLRTAYFFPLLISWASVSLIWLYALNPNFGF
                     FNYYLRRLGLDPPNWLVDRQWAMASLLFVDLWRTIGFTFIILLAGLQGVPRTLYEAAM
                     LDGAGSFRRFINVTLPMISPTLFFVLVLNFIGAFQIFEPMYIMTDGGPRNSTRSLVQH
                     IFETGFRSFEMGYASAMAIVVFVVIMAATLVQFAFGRYWVHYD"
     gene            4723..5580
                     /locus_tag="VHR64_01530"
     CDS             4723..5580
                     /locus_tag="VHR64_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3301529.1"
                     /translation="MTDHAVQGSGLRFGRVGGWVLNLILVVLGLLMLAPFYWMFITSV
                     LTTNDAFNLPPVWFPSEITFENYRRVFDLIPFWKMVLNSLKISGIITIGAVTTSTLAA
                     YAFARLRFPGRNLIFIILLSALMVPQQVTVIPTFILIRNLGLLDTHEAVYLPATINVF
                     GIFLLRQFFLSIPQELEDSARLDGAGHLRILLQIIVPLSGPAISALAIFVFQAAWNDF
                     FWPNLFLTSPGKMTLPLGLVSLKGQYGGGSAVALFAAMSMVAIPILILFSFTQRFLTE
                     SIATTGLKG"
     gene            5592..6299
                     /locus_tag="VHR64_01535"
     CDS             5592..6299
                     /locus_tag="VHR64_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301530.1"
                     /translation="MDGRQWTGEREQITDNSIDLMHLGRVIWADLPLLMVINLLATLA
                     GTVVMGVALAVAALAPIVAALLMGPVWIGAIAISRCILGGEAAGVRDLALLMRRYART
                     GIGLAMLPATVAAILIASAGIRSANPDDRWLMLPIIIDALVLVVLSLGAILAFPLAIM
                     TGVRGRDCWVAALALAGRNLLATLGVAALIVLLALSARFLGPMLLLIAAGPLAVLCTA
                     TTHGALQGNPTIDRRTR"
     gene            6296..7342
                     /locus_tag="VHR64_01540"
     CDS             6296..7342
                     /locus_tag="VHR64_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3301531.1"
                     /translation="MSRRVSDGRRVTLQDIADATGLSLASVSLALNDREGISSENRQR
                     VIQVARELNYERLASRPRTARRTVSLIVERLPVSVAADPFNRPIIEGLEAAARQHGYR
                     IMMEFVSPSDHPGSAHWSQGATDGVIILGGGDLSPEWVQAAVDAAIPVVMLDHVVPGL
                     DLPAVIPDNLAGAHSITRHLLDMGHRRIGFIRGPSKYWTLSERLAGFMLALQQAGVWP
                     REELMPPRVSHSEEKGYGEMQILLDLPKPPTAVVAVSDKTAVGAYRAIIERGLAIPQD
                     ISIVGFDDIEVARALNPPLTTVHVPGAEIGRVGFERLRHLIEGEENGLSRQIKWTIPT
                     RLVHRGSVRSVSGD"
     gene            7532..8503
                     /gene="accD"
                     /locus_tag="VHR64_01545"
     CDS             7532..8503
                     /gene="accD"
                     /locus_tag="VHR64_01545"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX3301532.1"
                     /translation="MRSFFHRSGKFQPLQSDNERAEAVPDNLCDLWVKCPKCNELIYQ
                     PELEHNMRMCPKCGHLHRMSARQRIEMLADPDSFQEWDAQIRTANPLGFAEPSGSYDT
                     KIAKTVARSGEQESLVTGRMTISGYGVAVAVADFSFLGASMGSVFGEKLARVAEHALE
                     ERLPVLTVNASGGARMHEGLFSLMQMAKTIAGLRLLGDAKLPHISLLTDPCYGGVSAS
                     YAMVADVIIAEPGALVGFAGPRVIEQITKQKLPEGFQTAEFLLGHGMIDGIVPRPELT
                     TELSRTLAHYSRIRPADPLLVSTDEPGRTVEIPVPTTEVTANGEVRS"
     gene            8500..9387
                     /locus_tag="VHR64_01550"
     CDS             8500..9387
                     /locus_tag="VHR64_01550"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX3301533.1"
                     /translation="MSSVINRARRPARSTEPEVVEAGNPGGLTAWDRVKLARHPDRPH
                     ALDYISELTTDFVELHGDRAFGDDHALIGGLANFAGRTVMVLGHQKGRGTNENIQRNF
                     GMARPEGYRKAERLMRHAEKFGMPVLTFIDTPGAEPGIGSEERGQSTAIAESLLTMAG
                     LRTMIVAVVIGEGGSGGALAIGVADRVLMLENAIYSVASPEASATILWKDVAKASDAA
                     ASMRITALDLYGFGMIDEIVPEENPAHERPREVIAATGSAVRRHLGDLHAQVQQENGL
                     DELVQHRYEKFRRIGAWTE"
     gene            9620..10141
                     /locus_tag="VHR64_01555"
     CDS             9620..10141
                     /locus_tag="VHR64_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3301534.1"
                     /translation="MPDLRTPERVDELVVAERLAGDATTDYGVPGVAPSVDAAPLDDA
                     DQRRLLTILAACWDALDAAADSAEGKELRKGPRGGGRSAPQILDHVIDAHFGYLKRLA
                     WREPHERDDDRSAEFAAVTETDARALAFAMSDEMPATGPRGGKLWTPRYFVRRAAWHI
                     LDHAWEIEDRLDG"
BASE COUNT         1751 a         3214 c         3346 g         1887 t
ORIGIN      
        1 gggcggcgac ctggtactcg atgccggtcc agacctcgtc gcagtagcgg accggcaccg
       61 tcggacggcc gcccttgggc caggtgcaga tcagcagacc attgtcatcg cgatcggcgt
      121 agacgcggaa gccgtgctca aaatcgccga atccctcgcg ggtgttgtat cggacgatgg
      181 agcggagcgt ggttcgcaca tgctcctgcg ggaggatgta gccgaggccg agcatatgcg
      241 cccaccactg tccgaagagc tgatcggaga ggcagccgtc cccgaactgg tcttcgagcg
      301 gcgtgttgtt gtcgaggagc tggatgtaat actcgccgtt ccagagcagc tcgtcgtagc
      361 gggcgctgcc ggctgtgaag agctcgtgga gatccgcagc gtacgcctcc tctcccacga
      421 tcttcgccag ctcctcgcct gcccggagcg cggcgagcca gagcccgccg atgtagatat
      481 tcgggccgaa gaagtggatg tcgtaggtgt tcccctgctc gccctcgagg acgccgtcac
      541 cgttctcgtc ccacgtgcgc cggatgtact cgagcaggcg acggacgttt tcccagtgat
      601 cccgcagcca aggaacatca ccgccctgcc gaacctcccg ataggtcttg aggaccgctc
      661 cgagcatgcc gtcgagggcc ggttggcgcg ggccgccgat cggctcgttc cagagctgct
      721 tcaggtaaag cggcaggatc gtccggtgcg gaatgtatcc ctccggcgcc tggacgtgct
      781 cgaactcggt ggtccgcatt cgctgctcga gtcgcgggaa caggcgcgag agggccatct
      841 cgtagttcca gacatgcgtg cagttgagtg ggcaggagcc gccgaagtca ccgttccaca
      901 tcgaggtcga tgccccgagc gatccctcga acccgaagaa gttgccatcc tcagtccaga
      961 aggtggtcgg gctgcgtatc aggctgccct gtgccgccat ggcctcgact agccagtcgg
     1021 gcagggagct gccgcgaagg gtttcttccc agcgacgcga gacccgctcg agctctgccc
     1081 gctccctgac gaggtggtcg gtcacgtcaa tcgcgtcggc gtagcgggtg gcgtaggcat
     1141 tcccgagcca gaaacgggaa tgaccgtaat cccgcatgcg gccgaactgg tcgaagttga
     1201 cgtaacggtt cgggaagaac caggagagga agaaggtgat ctgaatctcc tcgcccgggc
     1261 cgagatggaa cgggaccatc aggccgccgt tccaggtcgt gccggccggg ctcggaccgt
     1321 gctggaggat cggtagattc cggtacgcac gctgggtggg gctgtcctgc ccgacgaact
     1381 gccgggaggc gttcaacccc tccatgaaac gcaggaactg ctgcggcgtg gtccattgct
     1441 cgtagacgcg gacattattg gccagagccg tcagcatcat ctggccggcg cccgggtgat
     1501 cgtgcgggag ggacaggttc tccatcacga gcgaggtccg gtcgggcgag cggcggacgc
     1561 ggttcgtgtt gccgccgtag agcggattgc gattgccgga gatcggcgtc agcccgtccc
     1621 acccgaccgc gttctggagc gtggcggcga gggcgccttg caggttgaga tggccggcgt
     1681 tgcgaagcgt gaaggagaag aggatcgccg ggatcccgct ggcgacctca ttgagcggca
     1741 cgaacggcgt gtgcgcttcg aggctgacct cgagcggcag ctcgcgatcc aggtaccgga
     1801 cccgggcgaa ggggtagagg gctgagaact cggtccgctg cacgcccgga aacaactcga
     1861 ccagccggcg ctgatcctcc ggaatgtggt cgtcgttgac cagcggcgtc gggtccggcg
     1921 tcaacgcagc gacctcgcgc gactgcaaca ggcggacgac gttgagggga ggctcggtcg
     1981 aggtcacccg gatcgcgaag aaactgtccg gcacgaatcc ccggtggttg acctggttat
     2041 ggatttgcca ctggcgcagc ccgccgtctc cgccgagggc aatgtggccc gccccgacgc
     2101 caccgagggg catcgccacg tgccgcagcc gggacccgga ataggtaaag aaatcgctcg
     2161 tctcgcggtc tgtttcggag atctggtcgg tatcgaccgc ggtcatgccg tactcccctg
     2221 ttgttcgtgt gcggttcccg accggactca cataccccgg tcatctcagt cattcgcgtc
     2281 tgctgaaatg atttaccaca gttttagcta aaagtcttga ctctgtttta gcgtcagtgc
     2341 tatcgtgcag tccccagcgt gtgggcgcgt cgcagacgaa tattgaagga gcagtgtggc
     2401 tttggacgag aaaaccagga cgtcattgtc tcgccgttcg ttgctcaagg gcgccgcagc
     2461 caccggcatc gccggcgggg cagtgctcgc cggagcccgc tcttcgacgt ttgcggcgcc
     2521 ggcacttcgc atctccgcca cccgacccgg ttatcaggtc agcggcaagg tgacactggc
     2581 ctatctgggc accgccgacc aggcggccgt ttggaacaac ctcttcgccc tcttccagga
     2641 gaagtatccc gacatcgagc tcgaggcgca ggccaacccg gtcaatgact gggcggcctt
     2701 cttcgacgcc atttcgaccc agatcgccgg cggcaaggtt ccggacgtcg tccaggtcgc
     2761 caccgagggc cagcggctct ttgccagccg cggactctgc gagccgatca acgaccttat
     2821 cgatcgggac aaggcggagc tggccgacta cttcgaggac atccatccga agctgatcga
     2881 gtgggaccag acctacaaca cgaccaaaga cggctcgcgc tactacctgc cgggcgactt
     2941 caacacgatg ggctactggt acaacagcga ggtcttcacc accaacggca tcgacgagcc
     3001 gaccgacgac tggacctggg accaggtcct cgccgccggc gaagcactca aggccaaggg
     3061 catctacgcc atcaacgtca cgccggagta cttcatctcg gtcatgcctt ggctgctgac
     3121 caatggcgcc agcaccctga gcgcagactg gaccaccgct acggtgaaca ccccggaagc
     3181 gatcgaggcg gcgaccttca tgcgcaccct ggtcgagaag gaatacagcc cgctccccgg
     3241 cggcaccttc gaccagttca cggcgacggc gcagggcaag atggccgcat tcggcggtgg
     3301 gcggtggccg atcatcaaca tgcgcaacct gaaggtcgtc gacaagatga agatcgtcca
     3361 ctggccgcag aagaagcaga agggttcgcc ggtcgggtgg ggcgcctacc cgatcatgaa
     3421 gggaaccaag aacaaggaag ccgcctggac cttcctcaag ttccttgcct cgaaggaggc
     3481 cagcgtctac ttcgccgagc agggtggtac gatcgttccg ggacgaaagt cggtcgccaa
     3541 tagcgactcc tacctcgcga acgcgccgga gggcagcctc aagctctacg atgcgttgga
     3601 gtacgcgacg ccgatcccgt cgccggacaa gggcaacgtc gtgcaaagcg cgatccagga
     3661 cgctttcctg caggtcctga ccggcgcggt cgagccggaa gagggtctga acgcgctcaa
     3721 cgacgagatc cagtccaacc tctaggcagt cgctcgtatg cgggggtgag atggcagctc
     3781 aggcagatgc tggtgtggcc atctcgcccg gtatcccgtc gatgtcgcca acgcgccgag
     3841 ccggccggat tgggctggcc cggcgcgaga tgctgatcgg gtacgccttc ctcagcccgg
     3901 ctctgatcct cttcctcgtc ttcattgccg gtccgctgct gggcgcgatc gggctcagct
     3961 tctaccagtg ggatctcttc agcgaccgca cgttcatcgg attgcagaac taccgggcat
     4021 tgcgggacga cgaggtcgcg cggactgcga tccggaactc cttctacttc gccttctggt
     4081 cgatcgtgct gcacatcagc atcggcctgc tgctggcgct ggcggtcaac cgggcgataa
     4141 acggcgtgct ccgctacttc ctgcggacgg cctacttctt cccgttgctg atctcgtggg
     4201 cgtcggtctc actgatctgg ctgtatgcgc tgaacccgaa cttcggcttc ttcaattact
     4261 acctgcgccg gctcggtctc gatccgccga actggctggt cgatcgccag tgggcgatgg
     4321 cctccctgct cttcgtcgat ctctggcgca cgatcgggtt cacgttcatc atcctgctgg
     4381 ccgggcttca gggcgtgccg cgcacgctct acgaggcggc gatgctggat ggagccggat
     4441 cctttcgccg gttcatcaac gtaaccctgc cgatgatctc gccgacgctc ttcttcgtgc
     4501 tcgtgctcaa cttcatcgga gccttccaga tcttcgagcc gatgtacatc atgaccgacg
     4561 gcgggccgcg caacagcacc cgcagcctgg tccagcacat cttcgaaacc ggattccgct
     4621 ccttcgagat ggggtatgcc tcggcgatgg cgatcgtcgt cttcgtggta atcatggccg
     4681 cgacgcttgt ccagttcgcc ttcggccggt attgggtgca ctatgactga tcacgccgtg
     4741 cagggaagcg ggttgcgatt tggccgggtg ggcggatggg tgctcaacct gatcctggtc
     4801 gtgcttggcc tgctgatgct cgcaccgttc tactggatgt tcatcacctc ggtcctgacg
     4861 accaacgacg ccttcaacct gccgccggtc tggttcccga gcgaaatcac cttcgagaat
     4921 taccgtcgcg tgttcgatct gatcccgttc tggaagatgg ttctgaacag cctcaagatc
     4981 agcggcatca tcacgatcgg ggcggtgacg accagcaccc tcgcggccta tgccttcgcg
     5041 cgattgcgct tcccgggccg gaacctgatc ttcatcatcc tcctctcggc gctgatggtg
     5101 ccgcagcagg tgaccgtcat cccgaccttc atattgatcc gcaacctcgg cttgctcgac
     5161 acgcacgagg cggtttattt gccggcgacg atcaacgtct ttggcatctt tctgctgcgc
     5221 cagttcttcc tttcgatccc acaggagctg gaggatagcg cccgcctgga cggcgccgga
     5281 cacctgcgga tcctgctcca gatcatcgtg ccgctttccg ggccggcgat ctcggcactg
     5341 gcgatcttcg tgttccaggc cgcgtggaac gacttcttct ggccgaatct gttcctcacc
     5401 tctcccggaa agatgaccct gccgctcggt ctggtctcgc tcaagggcca gtacggcggc
     5461 ggctcggcgg tcgcgctctt cgcggcgatg tcgatggtgg ctattccgat cctgatcctg
     5521 ttttcattca cgcagcgctt cctgaccgag agcatcgcga ccacggggct gaagggatga
     5581 acagggggac ggtggacggg agacaatgga caggggagcg tgagcagatc actgataact
     5641 ccattgacct gatgcacctt ggccgtgtca tctgggccga tttgccgctg ctgatggtga
     5701 tcaatctgct cgcaacgctc gccggcacgg tggtgatggg agttgcgctg gcagttgcag
     5761 ccctggcgcc gattgtcgcg gcgctcctga tgggcccggt ctggattggc gcgatcgcca
     5821 tctcacgttg cattctgggg ggagaggcgg caggcgttcg agatcttgcc ctgctgatgc
     5881 gtcgctatgc gcggaccggc atcgggctag cgatgctccc ggcgaccgtc gcggccatcc
     5941 tgatcgcgtc ggccggcatc cggagcgcga accccgacga ccgctggctg atgctcccga
     6001 tcatcataga tgcgctcgtg ctggtggtct tgtcgcttgg cgcaatcctg gcgttcccgc
     6061 tcgcgatcat gaccggggtg cggggccggg attgctgggt ggcagcgctg gcgctcgccg
     6121 ggcgcaatct cctcgcgacg ctcggggtcg cagcgctcat tgtcctgctc gcgctctcag
     6181 ccagatttct tgggccgatg ctgctgctga tcgctgccgg accgcttgcc gtgctttgca
     6241 ctgcaacgac ccacggggcg ctccagggca acccaacgat cgatcggagg acacggtgag
     6301 ccgcagagtc tccgatgggc ggcgggtaac cctgcaggac atcgccgatg cgaccggctt
     6361 gtcactggcg agcgtctccc tggcgctgaa cgaccgggaa gggatttcct ccgagaatcg
     6421 ccagcgggta attcaggtgg cccgggagct gaactatgag cgcctggcct cccggccgcg
     6481 cacggcaagg cggacagtga gcctgatcgt cgagcgcctg ccggtttccg tcgccgcgga
     6541 tccgttcaac cggccgatca tcgagggtct ggaggcggcc gctcggcagc acggctatcg
     6601 gatcatgatg gagtttgtca gccccagcga tcatccggga agtgctcact ggtcgcaggg
     6661 ggcgaccgac ggcgtgatca ttctcggcgg aggcgacctg agcccggagt gggtgcaggc
     6721 ggcggtggac gcggcgatcc cggttgtgat gctcgatcat gtcgtgcccg ggctggatct
     6781 gccggctgtc attcccgaca acctggcggg cgcccactcg atcacccggc atttgctcga
     6841 catgggccac cggcgaatcg gtttcatccg gggaccgtcg aagtactgga cgctaagcga
     6901 gcggttggcc ggcttcatgc tcgcgctcca gcaggcggga gtctggccaa gagaagaact
     6961 catgccgccg cgtgtctccc attcggagga aaagggttac ggcgagatgc agatcctgct
     7021 cgatttgccc aagccaccta cggcggtggt tgctgtgagc gataagacgg cagttggcgc
     7081 ctaccgagcg atcatcgagc gtggcctggc gatcccgcag gacatctcga ttgtgggatt
     7141 cgacgatatc gaggtggccc gtgcgctcaa tccgccattg acgacggtcc atgtcccggg
     7201 cgcggagatc ggtcgggtcg ggtttgagcg gttgcggcac ttgatcgaag gtgaggagaa
     7261 cggcctgtcc cgccagatca agtggaccat tccaacgcgc ctcgttcatc gtggctcggt
     7321 tcggagcgta tccggggatt aagcatctcg agttcaggaa acaggagagc agcgtttcgc
     7381 cgcgtcattg atccctggaa ttcccataaa tcttgatctt caacccgtca ctcgtgcggg
     7441 ttttacgctt cccgactagt tgaacggaca cccgcgctcg tctatgctga gcccgccggt
     7501 gctggcggca tttctccggg caggtgaccc tatgcgttcc ttcttccacc gcagtggaaa
     7561 atttcaaccg cttcaatctg acaacgagcg agccgaagcg gtccccgata acctctgcga
     7621 tctgtgggtc aagtgcccaa agtgcaatga gctgatctac cagccggagc tcgagcacaa
     7681 tatgcggatg tgtccgaagt gcggtcacct gcaccggatg agcgcgcgcc agcggatcga
     7741 gatgctggct gatcctgatt cgtttcagga gtgggacgcg caaatccgca ccgccaatcc
     7801 gctcggcttc gcggaaccct ccggctccta cgacaccaag atcgccaaga cggtcgcccg
     7861 cagtggcgag caggaatcgc tggtgaccgg tcgaatgacg atcagcggct acggcgtggc
     7921 cgtggctgtg gccgatttca gcttcctcgg cgcgagcatg ggctccgtct tcggcgagaa
     7981 gctggctcgg gtggccgagc atgcgctcga ggagcggctg ccggtgctga ccgttaatgc
     8041 ctccggcggc gcgcggatgc acgaggggct cttctccctg atgcagatgg ccaagacaat
     8101 cgctggtctg cggttgctgg gtgatgcgaa gctgccgcac atttcgctac tcaccgatcc
     8161 ctgctacggc ggcgtcagcg ccagctatgc gatggtggcc gacgtgatca tcgcggagcc
     8221 gggcgcgctg gtcgggttcg ccgggccgcg ggtgatcgaa cagatcacga agcagaagct
     8281 acccgaggga ttccagacgg ccgagttcct gctcgggcac ggaatgatcg acggcatcgt
     8341 gccgcgcccg gagctgacca ccgagctcag ccgcaccctc gctcactaca gccggatccg
     8401 gcccgccgac ccgttgttgg tctcgaccga cgagccgggc cggacggtcg agatcccggt
     8461 tccgaccacc gaggtgaccg ccaacggcga ggtgcgctcg tgagcagcgt catcaaccgc
     8521 gcccgccgtc ctgccagatc gaccgagccg gaggttgtcg aagctggcaa tcccggcggg
     8581 ctgactgcat gggaccgggt caagctggcc cgccacccgg atcgaccgca cgccctcgac
     8641 tacatctccg agctgacgac cgatttcgtc gagctgcacg gtgatcgcgc gttcggcgac
     8701 gaccacgccc tgatcggggg tctggccaat ttcgccggac ggaccgtgat ggtgctcgga
     8761 catcagaagg ggcgcgggac caacgagaac atccagcgca acttcggcat ggcgcggccc
     8821 gaggggtacc gcaaggccga gcggctgatg cggcatgccg agaagttcgg gatgccggtg
     8881 ctgaccttca tcgatacgcc gggagcggag ccgggcattg gttcggagga gcgcggccag
     8941 agcaccgcga tcgccgagag cctgctgacc atggccgggt tgcggacgat gatcgtcgcg
     9001 gtcgtgatcg gcgagggcgg cagcggcggc gcgctggcga tcggcgtggc cgaccgcgtc
     9061 ctgatgctcg agaacgcgat ctattcggtc gcctcgcccg aggcctcggc cacgatcctc
     9121 tggaaggacg tcgccaaggc gtcagatgcg gccgcatcga tgaggatcac cgcgctcgac
     9181 ctctacggct tcggcatgat cgacgagatc gtgcccgagg agaatccggc ccatgagcgg
     9241 ccccgcgagg tgatcgccgc caccggctca gccgtccgcc gccatctcgg cgatctgcat
     9301 gcgcaagtcc agcaagaaaa cggtctcgac gagcttgtcc agcatcgcta cgagaagttc
     9361 cgccgcattg gagcgtggac ggaataggtt gagggtggag gggtcagggt tgagggaaga
     9421 cggaaaatcc ggaaaccgac ctgccaaaac caaaaaccaa gaacccaaaa cccaaattgc
     9481 gcttgagatc ggcaggaaac gggtcattgc gtccgcgttg gactggcccg gttgggctcg
     9541 ggttgggcgc gatgagcaag ccgcgctcga gacgctgtgc gcgtatggtc cgcggtatca
     9601 gcgggcgatc ggctcggcga tgcctgatct ccggaccccg gaacgcgtgg acgagcttgt
     9661 ggttgccgag cgattggccg gcgacgcaac gacggattac ggcgtgccgg gtgtggcgcc
     9721 atccgtcgat gccgctccgc tggacgatgc agatcagcgt cgcttgctga cgattcttgc
     9781 cgcgtgttgg gacgcactgg atgcggctgc cgattccgcc gagggcaagg aattgcggaa
     9841 gggtccccgc ggcggtggcc gctccgctcc tcagatcctc gatcatgtga tcgatgccca
     9901 cttcggctat ctcaagcgcc ttgcctggcg tgagccgcat gagcgcgacg acgatcgttc
     9961 cgccgagttc gccgcggtca cggagaccga tgcccgggcg cttgcgttcg caatgtcgga
    10021 tgagatgccg gcgactggtc cccgcggcgg caagctctgg acgccgcgct acttcgtccg
    10081 tcgggccgcc tggcacatcc tcgaccacgc ctgggagatc gaagaccgcc tcgatggctg
    10141 actgcggggc ggacgatcag ctgaggagca gtcgtggtcg ctcgcgcaga agctcttc
//
LOCUS       DATAOZ010000053         5407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3970750, whole genome shotgun sequence.
ACCESSION   DATAOZ010000053 DATAOZ010000000
VERSION     DATAOZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3970750"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VHR64_01560"
     CDS             complement(<1..781)
                     /locus_tag="VHR64_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX3301535.1"
                     /translation="MPVRARTLTTFAALLLLVTVQPGTGSWLDPHSSLVAAASTSLYV
                     STKGSDANSGSLHSPLRTLQRAANRVTPGTTVYVRAGTYKGFKLARSGTPDAPITFRS
                     YPGEHAVISGDPDHPSVVLLQNVHDVQMRDVTVQEAPFRKGSGIRVQYSDHILIADSV
                     IRRNDSFGILIDHGKYVRVQHNAISHNATGVRVRYGGRGVLIVRNRIYRNTRMIVNDA
                     TPGNDTGGQGIAFEKTTGGAIARGNQIWGNRAKSHDWGVDGN"
     gene            complement(846..1844)
                     /locus_tag="VHR64_01565"
     CDS             complement(846..1844)
                     /locus_tag="VHR64_01565"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301536.1"
                     /translation="MAAGLVVTQTPLRISFAGGGTDLPAFYELESGAVFSTAIDKYVY
                     VTVKRHGPVFDEQVRLNYSVSESVSEIAAVRNDIARECLRFLDIKPPIYVSVVSDLPD
                     SSGLGGSSAFAVGLLNALHTYRGERVSAGQLADEAAHVEIDILGQPIGKQDQYAAAIG
                     GLNLLRFSPGGDVAVVPQRMSRLTDELFKRMLLLWTGHQRRASSVLHEQLARTPEHAD
                     ILNRMRAQAFELQALMSAAEPDFAAFAGILDAGWALKRQLASSVSNPDIDRWYAAARE
                     AGADGGKLCGAGGGGFMLLLAEPDRHRTIVEALPELASVSVGYEAHGTRVLLAQLD"
     gene            complement(1941..2987)
                     /locus_tag="VHR64_01570"
     CDS             complement(1941..2987)
                     /locus_tag="VHR64_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3301537.1"
                     /translation="MNAQRVLVTGHKGYVGSVLVTVLREGGHEVHGMDTDYYRGCDLL
                     PPPVVPETMKDLRDVAVEDLKGFDAIIHLAALSNDPIGNLNSRWTASINVDGTARLAE
                     AARQAGVRRFLFASSCIMYGLAEGSDVDETSPLQPQTDYAASKVAAEEIISHMAGDDF
                     VPVKLRNGTIYGVSPRMRFDTVLNSLVGAALTTGRITVLGDGSPWRPVVHLRDVARAF
                     RAVLEAPEADIANQAINIGCEELNIQVAKLAEAVQEAIPEAALEILGRAEADQRTYHT
                     NFAKFARLFPDFAFEFDPRTGAAELADGLRAVGISEEDYRGERFTRLKRIRQLVEDGL
                     VDDQLRWLAAREAA"
     gene            complement(2984..4207)
                     /locus_tag="VHR64_01575"
     CDS             complement(2984..4207)
                     /locus_tag="VHR64_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX3301538.1"
                     /translation="MIDPVIARPRQAVILAGGRGTRMQPLTDHLPKAMIEFHGRPFLA
                     YLIEQLAEQGFERVLLLLGYLPEVIRGHFGDGSGLGISIDYSVSDPDDLTAKRVQIAA
                     DRLDDALMLMYCDNYLPLDMAAHWDAYVENGLPAMTTVYANEDGYSRDNVVVNPDGRL
                     EVFDRSRSAQGLKGVEIGYAILPKSVLGRLPADGNELIEQALYPGLAAEGAISARVSR
                     HRYYSVGSMHRLPLTEQFLARIPTVILDRDGVLNERPPRAEYVRHPAQFHWLAGALQA
                     LALLSNAGFRVIVVSNQAGIGRGLMSIDDLEKVHDRMRADATEAGGHIEAIYFCPHDW
                     NEGCECRKPKPGMIFRAQRDFQLDLSRTTYIGDDERDGEAAHAAGCRYLSVTESSSLL
                     DHARSLTRTPERISL"
     gene            complement(4204..>5407)
                     /locus_tag="VHR64_01580"
     CDS             complement(4204..>5407)
                     /locus_tag="VHR64_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3301539.1"
                     /translation="AGADFAKGSRFLHGGGTSDMPFHRRWGNASFVRLVRMLFGGRYT
                     DLCYGYNAMWRDVLPALELDGQGFEIETMMNIRVLRAGLKVVEVPSFEDRRVMGVGRL
                     RTVPDGWRVLRTIWRERSRPIPVVSAGDPGEVVAIPIQREPVAIAMSSVLQQAREGNG
                     NGHPRGGNAYPAAAGNGHSVAEMSDPVWETLSEPPSGATSALRETWAALTDTYVQRLQ
                     EVLARVDREAIARVVEILRTARDREATVFLGGNGGSAATASHWTNDLGKATKLSGRQP
                     LRVMNLSDNVPWLTALANDEGYDRTFAGQLENFARPDDVLMIISASGNSPNILRALEM
                     AASRQMVTIGLLGFDGGMARELVAEALWVETELGAYGLVETAHAALADIITACLIADR
                     SPAEVTSA"
BASE COUNT          986 a         1782 c         1649 g          990 t
ORIGIN      
        1 cgttgccgtc gacaccccaa tcgtggctct tggctcgatt gccccatatc tgatttccac
       61 gcgcgatggc gccgcccgtg gtcttttcaa aggcgatgcc ctggcccccg gtgtcgttgc
      121 caggcgtcgc gtcgttcacg atcatccgcg tgttgcggta gatgcggttc cggacgatga
      181 ggacgcctcg accgccatac cggacgcgga cgcccgtggc gttgtggctg atcgcgttgt
      241 gctgcactcg aacgtacttg ccgtgatcga tcaggattcc gaagctgtca ttgcgccgta
      301 tcacggaatc cgcaatgagg atgtgatcgg agtactggac gcgaatgccc gagcccttcc
      361 ggaatggtgc ttcctggact gtgacgtctc gcatctgcac gtcatggacg ttctgcagca
      421 ataccacgct tgggtgatcc gggtccccac tgatgacagc gtgctcgccc ggataggaac
      481 gaaaggttat aggtgcgtcc ggcgtgcccg agcgggccag cttgaacccc ttataggtgc
      541 ccgctcgcac gtagacggtc gtgcctggcg tgacgcgatt ggcggctcgc tgcagcgttc
      601 gcagcggcga atgaagcgag ccagagttgg cgtcactgcc tttcgtcgat acatacaggc
      661 tcgtcgatgc ggctgccacc agactcgaat gaggatcaag ccacgatccc gtgccgggtt
      721 gcacggtgac gaggaggagg agcgccgcga aggtcgtgag ggtgcgagct cggaccggca
      781 tggtcaatac ggtatagcct ggcaacaggt cgcggattga tcagatcgtc ctgttgcggt
      841 cctattcagt ccagctgggc cagcaggacg cgcgtaccat gggcctcata tccgaccgag
      901 acggacgcca gctccgggag ggcctcaacg atggtccggt gcctgtccgg ctcagcgagc
      961 agcagcatga agccaccgcc gccggcaccg catagcttgc cgccatcggc tccggcttcg
     1021 cgcgcagcgg cgtaccagcg gtcgatatcc ggattgctga ccgaggaggc cagctggcgc
     1081 ttgagggccc agccggcgtc caggatcccg gcaaacgccg cgaaatccgg ctcagccgcc
     1141 gacatcagtg cctggagctc gaaggcctgt gcccgcatgc gattcaagat gtcggcatgc
     1201 tccggcgtgc gcgcaagctg ctcgtgcagc accgaactcg cgcgccgctg atggccagtc
     1261 catagcagca acattcgctt gaatagctcg tcggtgagcc ttgacatccg ctgcggcacc
     1321 acggcgacat ccccgccagg gctgaagcgc agcaggttca aaccgccaat tgcggccgcg
     1381 tactgatcct gcttgccaat cggttgtccc agaatgtcga tctcgacgtg cgcggcttcg
     1441 tcggcgagct gtccggcgct gacacgctcc ccgcgatagg tgtgcagggc gttgagcaac
     1501 cccaccgcga atgcagatga ccctcccaag ccggatgaat caggaaggtc gctcacgacg
     1561 ctgacgtaga tcggcggctt gatgtcgaga aagcgcaagc attcacgggc gatgtcgttg
     1621 cgcactgctg cgatctcgct caccgactca ctgactgaat aattcagccg cacctgctcg
     1681 tcgaacaccg ggccatggcg ctttaccgtc acgtacacgt acttgtcgat cgcggtgctg
     1741 aagacagcgc cggattcgag ttcgtagaac gccgggagat ccgtgccgcc tccggcgaaa
     1801 ctgatccgca atggcgtctg tgtgacgacc agacccgccg ccatcgagcc gggcgtccta
     1861 ctcacggtct gaccaacgag tggcgggtcg tccgcaattt gccgcttgcg gcctgggctg
     1921 aatccgcatt accggccggc tcatgccgct tcgcgcgccg ctagccatcg aagctgatcg
     1981 tcaaccagcc cgtcttcaac cagctggcga attcgcttga gccgtgtgaa gcgctcgccc
     2041 cggtaatcct cttctgatat gccgaccgcc cgtagcccat ccgccagctc ggcagcgccg
     2101 gtacgcggat cgaactcgaa cgcgaaatcc gggaagaggc gcgcgaactt cgcaaagttg
     2161 gtgtggtagg tgcgttgatc cgcctcggcc cgacccagaa tctcgagcgc cgcctcagga
     2221 atcgcctcct ggaccgcttc tgcaagcttc gcaacctgaa tgttgagctc ctcgcagccg
     2281 atgtttatcg cttgattggc gatatcggcc tccggggctt cgagcacggc cctgaacgcc
     2341 cgcgccacat ctcgcagatg cacgaccggt cgccaggggc tgccatcgcc gaggacggtg
     2401 atccgcccag tggtcagtgc cgcgcccacc aggctgttca gcaccgtgtc aaatcgcatc
     2461 cgcggggaca cgccgtagat cgtcccattc cgaagcttga cgggcacgaa atcgtccccg
     2521 gccatgtggg agatgatttc ctcggcggcg accttcgagg cggcgtagtc agtctgtggt
     2581 tggagcggcg acgtctcatc cacgtcgcta ccttccgcaa ggccatacat gatgcaggag
     2641 gaggcgaaca agaaccggcg aaccccggcc tgccgcgcag cctctgccag cctggcggtc
     2701 ccatcaacgt tgatcgatgc agtccaacga gagttcaaat tgccgatcgg atcgttgctg
     2761 agtgccgcca ggtggatgat cgcatcgaat cccttgaggt cttcgactgc gacgtcccgc
     2821 agatccttca tggtctcggg cacgaccggt ggaggcaaca ggtcgcagcc gcggtagtag
     2881 tcggtgtcca tgccgtggac ctcgtggcca ccttcccgaa ggaccgtcac gagcacagac
     2941 ccgacgtatc ccttgtgacc ggtgaccagg acccgctgag cgttcacagg ctgatccttt
     3001 ctggcgtgcg tgtgagcgag cgagcatggt ccagtagcga gctcgactcc gttaccgata
     3061 gatatcggca tcctgcggcg tgcgcagcct cgccatcgcg ctcatcgtcc ccgatatacg
     3121 tcgtccggct caggtcgagc tggaagtcac gttgcgcccg gaagatcatt ccgggcttcg
     3181 gcttgcgaca ttcgcagccc tcattccaat catgagggca gaagtagatg gcctcgatgt
     3241 ggccacctgc ctccgtggca tcagcccgca tccgatcgtg gaccttctca agatcgtcga
     3301 tgctcatgag gccgcggcca atccccgcct ggttcgagac gacgatgacc cggaagcccg
     3361 cgtttgacag caacgcgagt gcctggagcg cccccgccaa ccagtgaaac tgtgcggggt
     3421 ggcgaacgta ctccgcacgc ggcgggcgct cattgagcac accatctcga tccaggatca
     3481 ccgtcgggat acgagccaga aattgctcgg tcagcggcag gcgatgcatg gaccccacgc
     3541 tgtagtagcg atgacgggat accctggcag aaattgcgcc ctctgcggcc aggcccgggt
     3601 acagcgcttg ctcgatcagc tcgttcccat cagccggaag gcgcccaagg acggatttcg
     3661 gcaggatggc atagccgatt tcgacgccct tcagtccctg cgcactccgg cttctatcga
     3721 agacttccag gcgcccgtcg ggattgacga ccacgttgtc acgcgagtac ccgtcctcgt
     3781 tcgcgtatac ggtggtcatg gctgggagcc cgttctcgac atatgcgtcc cagtgggcgg
     3841 ccatgtcgag aggcagatag ttgtcgcaat acatcaacat caaagcatcg tcgagccggt
     3901 ccgcggcaat ctggaccctt ttcgcggtca ggtcatccgg gtcagacacg gagtaatcga
     3961 tggagatgcc caggccactg ccgtcaccga agtgcccccg gatgacctcg ggcagatagc
     4021 cgagcaggag caggactcgt tcgaatccct gctcagcgag ctgctcgatc agatatgcga
     4081 ggaaggggcg gccgtggaac tcgatcatcg ccttcggcag gtgatctgtg agcggttgca
     4141 tccgcgtccc gcggccaccc gccaggatga ccgcctggcg cggccgcgca atgactggat
     4201 ctatcaggcg gacgttacct cggctgggct acggtcggcg atcagacagg cggtgatgat
     4261 atcggccaag gcggcgtgag ccgtctccac gagcccatat gcacccagct ccgtctcgac
     4321 ccacagcgcc tccgcgacca gttcccgggc catgccacca tcaaaaccca gcaggccaat
     4381 cgtaaccatc tggcgcgacg ccgccatctc cagcgcgcgc aggatatttg gcgagttacc
     4441 gctggccgag atgatcatca ggacgtcgtc cggccgagcg aaattctcga gctgtcccgc
     4501 aaacgtgcgg tcgtaaccct cgtcgttggc cagtgccgtc aaccatggga cgttgtcaga
     4561 caggttcatg acgcgcaggg gctgcctgcc ggaaagcttc gtggccttcc caagatcatt
     4621 cgtccagtgc gatgccgtag cggcgctgcc gccgttgccc cccaggaaca ccgtagcttc
     4681 acgatcccga gcggtacgaa ggatctcgac gacccgtgcg atcgcctcac gatcgaccct
     4741 cgccaggacc tcctgcaacc gttggacgta ggtgtcggtc agcgcggccc acgtctcgcg
     4801 cagcgccgag gttgcaccac tgggcggctc tgacaacgtc tcccacaccg ggtccgacat
     4861 ctcggccacc gagtgaccgt tccctgcggc ggctggatag gcgttgcccc cgcgcgggtg
     4921 gccattgccg ttgccctccc gcgcctgctg cagaacgctg ctcattgcaa tcgccacggg
     4981 ttcacgctgg atggggatcg ccacgacctc tccaggatca ccggcggaga cgaccgggat
     5041 gggccgcgat cgctcacgcc agatcgtgcg cagcacccgc cagccatccg gcacggttcg
     5101 cagccgcccg acgcccatta cccgtcggtc ctcgaagctt gggacttcca ctaccttcag
     5161 gccggcgcgc aggacgcgaa tgttcatcat cgtttcgatc tcgaagccct gcccgtccag
     5221 ctccagagcc ggcaggacat cccgccacat tgcgttatag ccgtagcaaa ggtctgtgta
     5281 gcgtccgcca aacagcattc ggaccagccg gacaaagctc gcatttcccc agcgccgatg
     5341 gaatggcatg tcgctggtcc caccgccatg aaggaacctg gatcccttcg cgaagtcggc
     5401 acctgca
//
LOCUS       DATAOZ010000054         6276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2129771, whole genome shotgun sequence.
ACCESSION   DATAOZ010000054 DATAOZ010000000
VERSION     DATAOZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129771"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VHR64_01585"
     CDS             complement(<1..231)
                     /locus_tag="VHR64_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301540.1"
                     /translation="MSDAWSSKIAAFVVQALDLEIEPQDLIPEIVALKRDDASGISAV
                     ELQSSVGPTPFLVYHYQVVAGDQSQFNADIATL"
     gene            264..668
                     /locus_tag="VHR64_01590"
     CDS             264..668
                     /locus_tag="VHR64_01590"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX3301541.1"
                     /translation="MDLEQAQMLMDYARAATTSAYVPYSQFPVGAAVMTESGAIFTGA
                     NIENASYPLTCCAERTAVYAAINAGHKVITAVAVTAPKVVTVTPCGGCRQVLNEFKPA
                     DQDMAVILDGLDLTIITLGDLLPRAFGPQDLE"
     gene            complement(669..1814)
                     /locus_tag="VHR64_01595"
     CDS             complement(669..1814)
                     /locus_tag="VHR64_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301542.1"
                     /translation="MVGRLLAAATLSLLFALSLIGTARAAETKSGDSVSVPAGQTIQG
                     DLHVAGQDVSIAGTINGDLIGFAQHVTISGTVNGSVNLAAQDIEISGTVTNSVRAAGK
                     SVTVSGSVHGDLAVAADSITITSTGEVNGDVLVGSGDLRVAGPVGRDIRGSVSDLIVD
                     SRVGQDVRVSADSISLTGKARIGGDLRYSSGKAAVSAPGGRVSGQTVRTSQYWFAGGP
                     DIWSAITSHIVRTLLMLVTGLFVILFIPRASIAAADAIRIQLLASAVTGLLSFILWPV
                     LAVIFAVVLVGIPIALVGTVLLLCFAYLSQVFVGLAIGRVILPGSWRIGSRGFNILAM
                     AIGVILIGLIRMIPAPVVSPLVALIVAILGVGGILTALRNAHSSVPG"
     gene            complement(1817..2938)
                     /locus_tag="VHR64_01600"
     CDS             complement(1817..2938)
                     /locus_tag="VHR64_01600"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3301543.1"
                     /translation="MTTTVSLDQALEQIDHWIDPSGVAGAAVVVRSGGREIAKRCVGE
                     AQPGIPVAPDTLFGLASVTKPITASVVMMLVDEGRVGLDEPVVRFVPEFASAAPDGNP
                     QWEAARRMITVRQVLSHMSGLAEDLPPGRLRARDLPDLDTITDHLIALPLQFEPGTSL
                     LYSNAGYGLLGRLVNRVTGRDIWEVARDRLLAPMGLSGIVARPSAELDDRIATVADAG
                     SSGSPHESFNSRYWRDLAIPWGGLFGTASDIAAFAESFMLDNLNPVSTRARELMTTDQ
                     AGGVSGGLAMLKLVWHPAHWGLGWEVKGGKRRHWTGEYTSPQTYCHWGSAGTLVWADP
                     EIGVTVAVFGNRTTYSQWPFQPVARWARLSNAIIASRDR"
     gene            3014..3745
                     /locus_tag="VHR64_01605"
     CDS             3014..3745
                     /locus_tag="VHR64_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX3301544.1"
                     /translation="MKITIVADYDAMSQRAADIVTETLTRKPDCALCLPTGNTPLGLF
                     RILIDRITSGELDASRAQFFLLDEYVGIDDDDPESLTRWLKAEFLTPAGIPEQNIHEL
                     PTMANDLAAAARQYEADLAAAGGLELAVLGLGGNGHIAYNEPGSPAESRTRVLTLTDE
                     SIEQARGYFEGRTVPTQAISAGVATLLEARNIVLLVSGASKAGIVRDALYGPQTPQIP
                     ASFLQLAGSRVEVVIDEAAASDFPA"
     gene            complement(3728..4654)
                     /locus_tag="VHR64_01610"
     CDS             complement(3728..4654)
                     /locus_tag="VHR64_01610"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX3301545.1"
                     /translation="MPDYTRELEFAIATAIEAGEEIRRLYASNSAQTYTKADESPVTD
                     ADLASDRIIRARIRETYPNDAILTEEGVDDEARLSAERVWIVDPIDGTIQFVERTGQF
                     DVLMALVVDGRPVVSVMLQPPTGAFVGAVAGQGAIFGFAGSSERTVARLAPPGDHPRV
                     ATTIWLGAPESEPYLDRFADRLGVAPPEITQTGLIARGHLDPAMPALRDRADCRMILA
                     YERPLHGFLGIPIRGNGTMAWEWDYAAADLVINEAGGRFTNWYGDYFTYNKPVPRNPS
                     GLIIANSPELHEQMLTAIAPEIEPVNSSRGKV"
     gene            4807..>6276
                     /locus_tag="VHR64_01615"
     CDS             4807..>6276
                     /locus_tag="VHR64_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX3301546.1"
                     /translation="MAARTLPTTFSLGVCYYPEQWPEDRWDLYATRMRELGLAWARIG
                     EFAWSRIEPAPEQYTWDWLDRAIESLANQELGVVLGTPTGAPPPWLIEQHPEILPVDP
                     EGRTRNLGSRKHYDHASPVYREHSRRITQAMADRYGQHEAVIGWQIDNEWGESDSTCS
                     YGPLTAVAFRAWLRNRYGSLERLNEAWGGVFWSQEYSDWDQIDPPNLTMNDPNPSHTL
                     DFYRFSSDAIVDFQNLQVEVIRAASPGRFITHNAMGHFGEVDYYKCAETLDFMSWDSY
                     PTGQVDKDLLPDFDPDYWARTGHPDLTGLNHDLIRGLKPGPGPIVMEQQAGQINWAKT
                     NPLPPDGAVALWTIQAWAHGCDAVSYFRWRAAVGGQEIMHSGLLRHDETWDRGGIEVA
                     NLQITGAPLDPIPARVVLLHDYESLWISDLQPHAEGWSYWKQFTLFYSTLRRLGADVD
                     IRHVDSNLDGYDVIVAPAIQIIDHRRAEHLASATRNASLV"
BASE COUNT         1139 a         2122 c         1978 g         1037 t
ORIGIN      
        1 gagcgtcgcg atgtccgcgt tgaactgcga ttggtcccca gccacgacct ggtagtggta
       61 gaccaggaac ggtgtcggtc caacactcga ctgcagctcc acggcactga tgccggaggc
      121 gtcgtcgcgt ttcagtgcga caatctccgg aatcaaatcc tgcggctcga tctcgagatc
      181 gagcgcctgg acaacgaatg cggcgatttt tgacgaccat gcatctgaca tatggcgatg
      241 ataccgggaa aggctgacga ctgatggacc tcgagcaggc gcagatgctc atggactacg
      301 cccgcgctgc gaccacttcg gcctacgtgc cgtactccca gtttccggtc ggcgcggcgg
      361 tcatgaccga aagtggcgcg atcttcaccg gcgccaacat cgagaacgcg agctatccac
      421 tgacctgctg tgcggagcgg acggctgtct atgcggcaat caacgccggc cacaaggtca
      481 ttacggccgt agctgtcacg gcgcccaagg tcgttaccgt cacgccgtgc ggcggttgcc
      541 ggcaggtgct caacgagttc aagccggccg atcaggacat ggccgtgatc ctcgacggcc
      601 tcgacctgac catcatcacg ctcggcgacc tcctgcctcg cgcgttcggg ccgcaagacc
      661 tcgagtaatt atcccggcac ggaggaatgg gcgtttcgga gggcggtcag gattccgccg
      721 acgccaagga tcgcaacgat cagcgccacc agcggcgaga cgaccggcgc cggaatcatc
      781 cggatcagac cgatcaggat cacgccgatc gccatcgcga ggatgttgaa cccgcgcgat
      841 ccgattctcc agcttcccgg caggatcacc cgcccgatcg cgagtcccac gaagacctgg
      901 ctcaggtagg caaagcagag cagcagcacc gtcccaacca gcgcgatcgg tattccgacg
      961 aggaccaccg cgaatatcac ggccagcacc ggccagagaa tgaaggacaa tagcccggtc
     1021 accgcgctcg ccagcaactg gatgcggatc gcgtcggccg cggcaatcga ggctcgcggg
     1081 atgaacagga tcacgaacag gccggtcacc agcatcagca acgtgcggac gatgtggctc
     1141 gtgatcgccg accagatatc cggcccgccg gcgaaccagt attgactggt ccgcacggtc
     1201 tgtccgctga cccgaccgcc gggagcgctg accgctgcct tgccgctcga gtaccgcagg
     1261 tccccgccga tccgggcctt gccggtcagc gaaattgaat ccgccgagac gcgcacatcc
     1321 tgaccgactc gcgaatcgac gatcagatcg ctcaccgagc cgcgaatgtc gcgtccgact
     1381 gggccggcca cccggagatc accacttccg acgagtacat caccgttgac ctcgccggtg
     1441 cttgtgatcg tgatcgagtc ggcggcgacg gcgaggtcgc cgtgaactga cccggatacg
     1501 gtgacggact taccggcggc gcgcaccgag ttggtgacag ttccgctgat ctcgatgtcc
     1561 tgcgcggcga ggttgaccga tccgttgacg gtgccggaga tcgtgacgtg ttgggcaaac
     1621 ccgatcaggt cgccgttgat cgtgcccgcg atcgagacgt cctgcccagc cacgtgcaaa
     1681 tcgccctgga tcgtctgacc ggccgggacg gaaaccgaat cgcccgactt cgtttcggcg
     1741 gccctggcgg tgccgatcaa cgagagtgcg aacagaagcg agagtgtcgc ggctgccaac
     1801 aaccggccga ccatgctcag cggtcccggc tggcgatgat cgcgttcgag aggcgggccc
     1861 agcgcgcgac cggctggaac ggccactggc tatatgtcgt gcgattgccg aagacggcga
     1921 cggttacgcc gatttcaggg tcagcccaga ccagcgtccc ggccgagccc cagtggcaat
     1981 acgtctgcgg tgaggtgtat tcgccggtcc agtgccggcg cttcccgccc tttacttccc
     2041 agcccagtcc ccagtgcgcc gggtgccaca cgagcttgag catggccaaa ccgccggaca
     2101 cgccgccggc ctggtcggtg gtcatcaact cccgcgcccg tgtcgacacc ggattcaggt
     2161 tgtcgagcat gaagctctcg gcgaatgcgg cgatgtccga cgccgtaccg aaaaggccgc
     2221 cccacgggat cgccaggtcg cgccagtacc ggctgttgaa ggactcgtgc ggactcccgg
     2281 agctgccggc atcggccacg gttgcgatcc gatcgtcgag ctcagcactg ggccgggcga
     2341 cgatcccgct caggcccatc ggggcgagca ggcgatcgcg cgcgacctcc cagatgtcgc
     2401 ggccggtcac tcggttgacc agccggccga gcaagccgta cccggcgttg gaatagagca
     2461 gcgatgtgcc tggctcgaac tgcagcggca gggcgatcag gtgatcggtg atggtgtcga
     2521 gatcgggtag atcccgcgcc cgcagcctcc cgggcgggag atcctcggcc agcccgctca
     2581 tgtgcgaaag gacctgccgg acggtgatca tgcgccgcgc ggcctcccac tgcggattgc
     2641 catcgggcgc ggcactcgcg aactccggca cgaagcggac caccggttca tcgagcccga
     2701 ctcgaccctc atcgacgagc atcatcacga ccgatgccgt gatcggcttt gtaacggaag
     2761 cgaggccaaa cagcgtatcg ggcgcgaccg gaattccagg ctgtgcctcg cctacgcacc
     2821 gcttggcgat ctcccgccct cccgaccgga cgacaaccgc cgctccagcg acgccggacg
     2881 gatcgatcca gtgatcgatc tgctcgaggg cttgatcgag cgagactgtg gtagtcaacg
     2941 acaaagaccc cccgaaccgg gtaaagtggc cgccagtatc gggagtttat cagccacgag
     3001 ggtcagccca ttcatgaaga tcacgatcgt tgccgactac gacgccatga gccagcgcgc
     3061 cgccgatatc gtcaccgaga cgttgacccg aaaaccggat tgcgccctct gcctgccgac
     3121 cggcaacacc ccgctgggcc tgttccggat actgatcgac cgcattacca gcggcgagct
     3181 cgatgcctcg cgcgcccagt tcttcctgct cgatgagtac gtcggcatcg atgacgacga
     3241 cccggaaagt ctgacccgat ggctgaaggc ggagttcctg actcccgccg gcattcccga
     3301 gcagaacatc cacgaactcc cgaccatggc caatgatctg gccgcggccg cccggcaata
     3361 cgaggccgat ctcgcggccg ccggcgggct cgagctggca gtgctcggcc tcggcggcaa
     3421 cgggcatatc gcctacaacg agcctggttc gccggccgaa tcgcgcaccc gtgtcctgac
     3481 cctgaccgac gagtcgatcg agcaagcccg cggctacttc gaaggccgga cggtcccgac
     3541 gcaggcaatc agcgcgggcg tggcgacgct tctcgaggcg cgcaatatcg tgctgctcgt
     3601 ctccggcgcg agcaaggccg ggatcgtccg cgacgcgctt tacgggccgc agaccccgca
     3661 gataccggcg tcattcctgc aattggccgg ctcgcgggtc gaggtggtga tcgacgaggc
     3721 tgctgcctca gactttcccg cgtgaggagt tgaccggctc gatctcgggc gcaatcgccg
     3781 tcagcatctg ctcgtgcaat tcgggcgaat tggcgatgat cagaccgctc gggttgcgcg
     3841 gcaccggctt gttataggtg aagtaatcgc cataccagtt ggtgaaccgg cccccggcct
     3901 cgttgatgac gaggtcggcc gcggcgtaat cccattccca ggccatcgtg ccgttgccgc
     3961 gaatcgggat gccgaggaaa ccgtgcagcg gtcgctcgta agccaggatc atccggcaat
     4021 ccgcccgatc gcgcagcgcc ggcatcgccg gatcgaggtg accgcgcgcg atcagaccgg
     4081 tctgcgtgat ctccggcggc gcaaccccca ggcggtcggc gaatcggtcg aggtacggct
     4141 ccgactctgg cgcgccgagc cagatcgtgg tcgccacacg cgggtgatcg cccggcggcg
     4201 cgagcctggc tacggtgcgc tcgctgctcc cggcgaatcc gaagatagcg ccctgtccgg
     4261 ccacggcgcc gacaaacgcg cccgtcggtg gctgcaacat gacgctgacc accggtctgc
     4321 cgtcgacaac caacgccatc aacacgtcga actggccggt gcgctcgacg aactggatcg
     4381 tgccgtcgat cggatcgacg atccagaccc gctcagccga gagccgcgcc tcatcgtcga
     4441 cgccctcctc ggtcaggatc gcatcgttgg gatatgtctc gcggattcgg gcgcggatga
     4501 ttcggtccga ggcgaggtcc gcgtcagtga ccggcgactc gtccgccttc gtataggtct
     4561 gggcgctgtt ggaggcgtag agtcgccgga tctcctcccc tgcctcgatg gccgtggcga
     4621 tcgcgaattc aagctccctt gtgtaatcgg gcatcgaggt ctgcatgctc ccctggacgg
     4681 cggctcaatt ctgcgcggaa gcctagcacc acgcgtcttt gccgcactga catcgccgct
     4741 tgcgaccgat ggcccctaca ctttcgccgt catttcgtct cgatcgatcg acaaggagtt
     4801 gcccggttgg ccgcgcgtac attgcccacg accttctcgc tcggtgtctg ctattacccc
     4861 gagcaatggc ccgaggatcg ctgggatctc tacgctaccc gcatgcgcga gctcggcctg
     4921 gcctgggcgc ggatcggcga gtttgcctgg tcgcgcatcg aaccggcgcc ggagcaatac
     4981 acgtgggatt ggctggaccg cgcgattgag agtctcgcca accaggagct cggcgttgta
     5041 ctcggaaccc cgaccggcgc gccgccgcca tggctgatcg agcagcaccc ggagatcctg
     5101 ccggtcgatc ctgagggccg cacccgcaac ctcggctcac gcaagcatta cgaccacgcc
     5161 agcccggtct accgcgagca ctcccggcga atcacgcagg caatggccga tcggtacggc
     5221 cagcacgagg cggtcatcgg ctggcagatc gacaacgagt ggggcgaaag cgactcgacc
     5281 tgcagctacg gaccgctcac cgcagtcgcc ttccgcgcct ggctccggaa tcggtatggg
     5341 tcactcgaac ggctgaacga ggcgtggggc ggtgtcttct ggagccagga atacagcgac
     5401 tgggatcaga tcgatccgcc gaatctgacc atgaatgacc cgaacccctc ccacacgctg
     5461 gacttctacc gcttttcgag cgacgcgatc gtggacttcc agaacctgca ggttgaggtc
     5521 atccgagctg cctcgccggg tcgcttcatc acccacaacg ccatgggtca ctttggcgag
     5581 gtcgactact acaagtgcgc cgaaacgctc gatttcatgt cgtgggacag ctatccgacc
     5641 ggtcaggtcg acaaggatct gctgcccgat ttcgaccccg attactgggc gcgcaccgga
     5701 catcccgacc tgaccggcct caaccacgac ctgattcgtg gcctgaaacc agggccgggg
     5761 cctatcgtga tggagcagca ggccgggcag atcaactggg cgaagacgaa tccactgccg
     5821 cccgacggag ccgtcgcact ctggacgatc caggcctggg cgcatggctg cgacgcggtc
     5881 agctacttcc gctggcgcgc ggcggtcggt ggtcaggaga tcatgcattc cgggctgctt
     5941 cggcatgacg agacgtggga tcgcggcggc atcgaggtcg ccaatctcca gatcaccggc
     6001 gcgccgctcg atcccatccc ggcgcgggtt gtcctgctgc acgactacga gagcctctgg
     6061 atctctgacc tccaaccgca tgccgagggc tggagctact ggaagcagtt caccctcttc
     6121 tactcgacgc tccgtcgcct cggcgcggat gtcgacatcc gccacgtcga cagcaatctc
     6181 gacggctacg acgttatcgt cgcgccggcg atccagatca tcgatcaccg ccgggccgag
     6241 catttggcga gcgcaacgcg caacgcgagc ctcgtc
//
LOCUS       DATAOZ010000055         6291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5550308, whole genome shotgun sequence.
ACCESSION   DATAOZ010000055 DATAOZ010000000
VERSION     DATAOZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5550308"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="VHR64_01620"
     CDS             <1..1671
                     /locus_tag="VHR64_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3301547.1"
                     /translation="RSIDELLLEWEHGRLTRRSVLRRAAALGLTIPALAGLMARAGSG
                     RAAAASLNLFQDDPASGTPGGTLKIATIGEAPHLDEHQSTAEIIALIGYCAYEGLFTY
                     DATYQAIPELVAEHTVSEDGLTHTMKLRQGVKFHNGDELKAADAIASVERWGRISGVG
                     KRLMEKTTGITAADDYTLEWKLSEPYGTILIALAHNTQACTIHPKSILDAAGDEPMTD
                     AAQYIGTGPYKLVEWQRDSVMRFERFADYQSAGETAQGYGGKKYAYADKIEFYPVPDE
                     AARVAGLQAGDYHIGLDIGNDQYEVLKDYDGVIAEILTPTNWDVFFLNWESPMFKDNL
                     AMRQAIQLAMDPTPQLQSGRGGGDFIRLDPGLMMQQTAWYTKAGEEHYNVYDPEAAKA
                     KLQEAGYNGEPLRFMTTQEYSYMYGEAIVAKQQLEDIGITIDFRVTDWATVLENRAKK
                     DAWEMFGTGHGFVPDPSQVSYVGQMNIYPGWWSSESSLALAADLLAESDFDTRFPIWE
                     KIQTAHYTEIPAIKIGDSSNVSFRSASLGGWDTQFERGPKFWNFWIKE"
     gene            1755..2705
                     /locus_tag="VHR64_01625"
     CDS             1755..2705
                     /locus_tag="VHR64_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301548.1"
                     /translation="MTAYILRRLIMLIPVILIVGIIVFALIHLTPGDPAAVILGDQAT
                     PEAVANLREQLGLNDPLPVQFIHWFSGVLHFDFGESIFMGEPVTRALLDRAQPTILLT
                     IYALMFQIAIGVPAGVISAVKHNSIVDRIVTVVAISGAAIPTFFLGILLILFFAVRLG
                     WLPSGGYTPLHEDPIAHFKKMLMPAFALGFSSAGLLARLVRSSMLDVMREDYVRTALA
                     KGLPMRLVVIRHALRNALIPALTVIGTSVGALLGGAVVTETVFTIPGMGRLVVQSISR
                     RDYPVIQGAVMVIAMTYVIVNLVVDLLYVYIDPRVRLGGE"
     gene            2705..3610
                     /locus_tag="VHR64_01630"
     CDS             2705..3610
                     /locus_tag="VHR64_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301549.1"
                     /translation="MAESTLTPAAAVDPSALLESTPRSRRSAWISVLISNPVTIAAAV
                     ILAVITLAAIFAPLLTSHDPTFLDPAVRLMSPSTDYILGTDDRGRDIFARVIYGGRTS
                     LLIGLSVTAAAALMGSLLGLVSGYYPRLDSLIMRAMDGLMAFPSILLAIAIMASLGPS
                     AINVFIALAVVYTPTVARLVRSTTLVTKQQAYVESARSIGMSDRAILLKYIFPNGISP
                     LIVQCTFVVAYAIISEASLSFLGAGVDPETPTWGNMLRDGQRVLQRAWWLAVFPGAAL
                     VLTVLSLNLLGDGLRDALDPRARKR"
     gene            3638..4705
                     /locus_tag="VHR64_01635"
     CDS             3638..4705
                     /locus_tag="VHR64_01635"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX3301550.1"
                     /translation="MLAIDAHLDLAMNAVLWDRDLKLSAHETREIERAEGMTAKGRCA
                     GTVGIPDLRAGEFGLVFATVLARTNQGLNSGIDFRTQDNSYAQAQGQLALYREFERQG
                     IMRMIRTAADLRQWKSEWDANPATTPIGYVLLMEGGDPIVEPGQARCWFDDGLRMVGL
                     AHYGPSQYAYGTASAGPITARGRELLKVMDEVGMIQDMSHLTDQSFYEALDLFQGPIF
                     ASHSNCRALVPGDRQITDDMIKRMVERDAVIGAVFDAWMLQPNWERGVTTNENVTIAN
                     VVDHIDHVCQIAGNADHAAIGTDLDGGYGIEQTPKDLDTIVDVQKVPDLLRQRGYAEA
                     DIAKIMHGNWLRFLERALPAS"
     gene            complement(4742..5584)
                     /locus_tag="VHR64_01640"
     CDS             complement(4742..5584)
                     /locus_tag="VHR64_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301551.1"
                     /translation="MERDSPCRLFVYLAREAPVGVVLRRGPSDWVRLSLWHTDADRFE
                     HGQWMRARVFERRSDLSADGSLFVAFVRGSAGPSQGNTDTWAALSRPPWFTALALWFV
                     GGTYYTGGYFPERGQLWLGWDSPEPDQGRLPEWLKMTSERPPYIDGTNDWTDRTVWMN
                     RLLRDGWTRREGAEPETWERSNPAGDMTLAMRIVTNIEFRAYGGRHVIEYAVQADPDE
                     QEVDLGRATWADWDQRGRLIVARDGRLLHWQPSGVTEIADFNDQSPDPQPAPSWATTW
                     PRRL"
     gene            complement(5658..5885)
                     /locus_tag="VHR64_01645"
     CDS             complement(5658..5885)
                     /locus_tag="VHR64_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301552.1"
                     /translation="MNPMSDKLYRDWRANELSREIAHETRVLSALQAQSTEPIHRTAR
                     FSIQQWLVAMSVNAMVKVRHFSVSASRPAAH"
     gene            complement(6063..>6291)
                     /locus_tag="VHR64_01650"
     CDS             complement(6063..>6291)
                     /locus_tag="VHR64_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3301553.1"
                     /translation="VPDDGPASGWWEPLGLTAREGEIFLLLRDRLTNKEIGDRLFISP
                     RTASTHVASILAKLEVSSRRDIPTVAKRLGV"
BASE COUNT         1094 a         2088 c         1948 g         1161 t
ORIGIN      
        1 cgttcgatcg atgagttact gctcgaatgg gaacacggtc gcctcactcg tcgctcagtg
       61 ctccggcgcg ctgcggcgct cggactcacg atcccggctc tggccggact tatggcccgc
      121 gcgggaagcg gacgggcagc cgcagcgtcg ctcaacctct tccaggatga tccggcatca
      181 ggcacccctg gcggcacgct caagatcgcc acgatcggtg aggcgccgca cctcgatgag
      241 catcagtcga ctgcagagat catcgctctg atcggctact gcgcatacga ggggctcttc
      301 acgtatgacg ccacctacca ggcgatcccg gagctggtcg ccgagcacac cgtcagcgag
      361 gacggcctga cccacaccat gaagctccgc cagggcgtca agttccacaa tggtgacgag
      421 ctgaaggcag cggacgcgat cgcctcggtc gagcgctggg gtcggatcag cggcgtcggc
      481 aagcgcctca tggagaagac gaccggcatc accgcggcgg atgactacac gctcgagtgg
      541 aagctgtccg agccgtacgg cacgatcctg atcgcgctcg cgcacaacac ccaggcctgc
      601 acgatccacc ccaagtcgat cctcgatgcg gctggtgacg agccgatgac cgacgctgcc
      661 cagtacatcg ggaccggccc gtacaagctg gtcgagtggc agcgggactc ggtcatgcgc
      721 ttcgagcggt tcgccgacta tcaatcggcg ggagaaacgg cgcaggggta cggcggcaag
      781 aaatacgcct acgccgacaa gatcgagttc tatccggtgc cggatgaagc cgcgcgcgtc
      841 gcaggtctgc aggctggcga ctaccacatc ggcctggata tcggaaacga ccagtacgag
      901 gtcctcaagg actacgacgg cgttatcgcc gagatcctga ccccgaccaa ctgggacgtc
      961 ttcttcctga actgggaatc gcccatgttc aaggacaacc tggcgatgcg ccaggccatc
     1021 cagctcgcga tggacccgac cccacagctc cagtccggtc gcggcggcgg tgacttcatc
     1081 cggctcgatc cgggcctgat gatgcagcag acggcgtggt acaccaaggc gggcgaggag
     1141 cactacaacg tctacgatcc ggaagcagcc aaggccaagc tgcaggaggc tggctacaac
     1201 ggcgagccgc tccggttcat gacgacccag gaatactcct acatgtacgg cgaggcgatc
     1261 gtcgccaagc agcagttgga ggacatcggc atcaccatcg acttccgggt caccgactgg
     1321 gcgaccgtgc tcgagaaccg ggccaagaag gatgcctggg agatgttcgg caccgggcac
     1381 ggcttcgtcc cggatcccag ccaggtctcc tacgtcggcc agatgaacat ctatcccggc
     1441 tggtggagct cggagagtag cctcgccctg gccgcggatc tgctcgcaga atccgacttc
     1501 gatacccggt tccccatctg ggagaagatt cagacagccc actacacgga gattccggcg
     1561 atcaagatcg gcgattccag caacgtctcc ttccgctcag catcgctcgg cggttgggac
     1621 acccagttcg agcgtggtcc gaagttctgg aacttctgga tcaaggagta gagcgcggcg
     1681 actggttcat tgggggtgct ccgtcttccg gggcacccct ttgaccatca ggatcgtcgc
     1741 gcctcgagga ctctatgact gcgtatatcc tccggcgact tatcatgctg atcccggtca
     1801 tcctgatcgt tgggatcatt gtcttcgctc tcattcacct gacccccggc gatccggccg
     1861 cagtcatcct cggtgatcag gccacacccg aggcagttgc aaacctgcgc gagcagcttg
     1921 ggctcaatga tccgcttccg gtccagttca tccactggtt cagcggcgtg ctccattttg
     1981 actttggcga atcgatcttt atgggcgagc cggtcacacg ggcgttgctg gaccgggccc
     2041 agccgaccat cctgctgacg atctatgcgc tgatgttcca gatcgcgatc ggtgtcccgg
     2101 ccggtgttat ctccgccgtc aagcacaact cgatcgtcga tcggattgtg accgttgtcg
     2161 cgatttccgg cgcagcgatc ccgaccttct tcctcggcat cctgttgatc ctcttcttcg
     2221 cggtccgtct ggggtggctt ccctccggcg gctacacgcc cctgcacgag gacccgatcg
     2281 cgcatttcaa gaagatgctg atgccggcct tcgcgctcgg attctcttcg gcagggctcc
     2341 tcgcccgcct ggtgcgatcc tcgatgctcg atgtcatgcg tgaggactac gtgcggaccg
     2401 cgcttgccaa ggggttgccg atgcggctcg tggtcatccg ccacgccctg cgcaatgccc
     2461 ttatcccggc cttgaccgtc atcggtacct ctgtcggcgc actgcttggc ggcgcagtcg
     2521 tcactgagac ggtcttcacg atcccgggca tgggccggct ggtcgtacag agcattagcc
     2581 gtcgtgatta ccccgtcatc cagggtgcgg tgatggtcat cgcgatgacc tacgtaatcg
     2641 ttaacctggt cgtggatctg ctttacgtgt acatcgatcc tcgcgtgcgc ctgggagggg
     2701 agtgatggcc gaatcaaccc tcacacctgc cgcggccgtc gatccgtctg ccctgctcga
     2761 gagcactccg cgctcccgtc gatcagcgtg gataagcgtg ctgatcagca atccggtcac
     2821 gatcgcagcc gccgtcatcc tggccgtgat caccctggcc gcgatattcg cgccgctctt
     2881 gacctcgcat gatccgacgt ttctcgatcc ggccgtgcgg ctgatgagtc cgtcgacgga
     2941 ctacatcctc ggcacggacg accggggccg ggacatcttc gcgcgggtga tctacggcgg
     3001 gcgtacatcg ctcctcatcg gactgagcgt gaccgccgcc gcggccctga tgggctcact
     3061 gctcggactg gtctccggct actacccgcg cctcgactcg ctgatcatgc gagcgatgga
     3121 cggcttgatg gccttcccaa gcatcctgct cgcgatcgcg atcatggcca gtctcggtcc
     3181 atcggcgatc aatgtcttca tcgcgctggc cgtcgtctac accccgactg tcgcccggtt
     3241 ggtccgctcg acgacgctcg tgacgaagca gcaggcctat gtagagtcgg cccgctcgat
     3301 cgggatgagc gaccgcgcga ttctcctcaa gtacatcttc ccgaacggga tctcgccgct
     3361 gatcgtgcag tgcaccttcg tggtggccta cgcaatcatc tccgaggcgt cgctctcgtt
     3421 ccttggcgcc ggcgtcgatc cggagacgcc gacttggggc aacatgctgc gtgacggcca
     3481 acgggtgctg cagcgcgcct ggtggctggc cgtcttcccc ggcgccgccc tggtgctgac
     3541 tgtgctctcg ctcaacctgc tcggcgacgg acttcgggac gccctcgatc cccgcgcccg
     3601 caaacggtaa ttggcttaat tcgattcagg aggacctgtg ctcgctattg atgctcacct
     3661 tgacctggcc atgaacgccg tgctctggga tcgcgacctc aagctcagcg cgcacgagac
     3721 gcgcgagatc gagcgcgccg agggcatgac ggcgaagggc cgctgcgccg gcaccgtcgg
     3781 catccccgat ctgcgcgccg gcgaattcgg gctcgtcttc gcgacggtcc tggcccgcac
     3841 caaccagggc ctcaacagcg ggatcgactt ccgcacgcag gacaactcgt acgcccaggc
     3901 gcagggccaa ctcgcccttt atcgcgaatt cgagcggcag ggcatcatgc gcatgatccg
     3961 caccgccgcc gatctcaggc agtggaagtc ggagtgggat gccaacccgg ccaccacgcc
     4021 gatcgggtac gtcctgctca tggagggcgg tgacccgatc gtcgagccgg ggcaggcccg
     4081 ctgctggttc gatgacggcc tgcgcatggt cggcctcgcc cattacggcc caagccagta
     4141 tgcatacggc accgccagtg ccggcccgat taccgcgcgc ggcagggagc tgctcaaggt
     4201 gatggacgaa gtcgggatga tccaggacat gtcccacctg accgaccaga gcttctacga
     4261 ggcgctcgac ctgttccagg gaccgatctt cgccagccat tccaattgcc gcgccctcgt
     4321 ccccggcgat cgccagatca ccgacgacat gatcaagcga atggtcgagc gggatgcggt
     4381 gatcggcgcg gtcttcgatg cctggatgct gcagccgaac tgggagcgcg gcgtgacgac
     4441 caacgagaat gtcacgatcg ccaacgtcgt cgaccacatc gaccatgtct gccagatcgc
     4501 cggcaatgcc gatcatgccg cgatcgggac cgatctcgac ggtggctacg gcatcgagca
     4561 gacgccgaag gatctcgaca cgatcgtcga cgtccagaag gtgccggatc tgctccgcca
     4621 gcgcggctac gcggaggccg acatcgccaa gatcatgcac ggcaactggc tgagattcct
     4681 ggagcgcgcc ctgcccgcga gctgattacc cccgacccgc tcgccggtac aacaccaatc
     4741 cctacaggcg tcgcggccaa gtggtggccc atgacggtgc tggttgcggg tcgggcgatt
     4801 ggtcgttgaa gtcggcgatc tcagtgacgc cggacggctg ccagtggagc aggcggccgt
     4861 cgcgggcgac gatcaggcgg ccgcgctgat cccaatccgc ccaggttgcc cggccgagat
     4921 cgacctcttg ttcgtctgga tccgcttgga cggcatactc gatcacatgg cggcctccgt
     4981 acgcccggaa ctcgatgttg gtcacgatcc gcatcgcgag cgtcatgtct ccggctgggt
     5041 tgctgcgctc ccacgtctcc ggctcggctc cttcgcgccg ggtccaacca tcgcgcagca
     5101 accggttcat ccagacggtt cgatcggtcc agtcattggt gccgtcgatg tatggggggc
     5161 gctcgctggt catcttcagc cactctggca gccggccctg atccggctca ggcgagtccc
     5221 agccgagcca gagttggccc ctctccggga agtagccacc tgtgtagtac gtgccgccga
     5281 cgaaccagag tgccagcgcc gtgaaccagg gcggacggct gagcgcggcc caggtatcgg
     5341 tattcccctg actcggaccc gccgatccgc gaacgaaggc cacgaagagc gagccgtcgg
     5401 ctgagaggtc gctgcgccgc tcgaagactc gtgcccgcat ccactgtccg tgctcgaacc
     5461 ggtccgcatc cgtgtgccag aggctcagcc gaacccaatc gctcggtccg cgccgcagca
     5521 ccacgccgac cggcgcctcc cgggcgaggt agacgaacaa ccggcacggc gaatcccgct
     5581 ccacgctcta aatctgctcc cattcagtcc gcgacattgc cccgggtcca gtatgccgga
     5641 tgcttgcgcc cgagatgtca gtgcgctgcc gggcggctgg cagacacgga gaaatgtcga
     5701 accttcacca tcgcattgac cgacatcgca acgagccact gttgaatgct gaatcgcgcc
     5761 gttcggtgga tcggctcggt ggactgtgct tgcagggccg aaagaactcg ggtttcgtgt
     5821 gcgatctcgc gcgagagctc attcgctcgc cagtcacggt agagcttgtc ggacattgga
     5881 ttcatctcgc tgctccctgg tcgcgtcgaa ctgccgtact gcgatcatgg gcgcgctggg
     5941 agttactgtc atcggtcgga tctgcgtaat cgcataccag caggccacgt gaggtgtacg
     6001 tcactgttgc gtagtgacca gggagtagcg cagatactcc tgtgaggtgc cggctctgtg
     6061 actcaaacac caagacgctt ggcaacggtt gggatgtcgc gccgggagct gacctcgagc
     6121 ttggccagga tggacgcaac gtgtgtactg gcggtccgcg ggctgatgaa gaggcggtcg
     6181 ccgatctcct tgttcgtcaa ccggtcgcgc agcagcagga agatctcgcc ttcccgagcg
     6241 gtcaatccaa gcggttccca ccagccggat gcggggccgt cgtcaggcac c
//
LOCUS       DATAOZ010000056         7883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5551225, whole genome shotgun sequence.
ACCESSION   DATAOZ010000056 DATAOZ010000000
VERSION     DATAOZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5551225"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VHR64_01655"
     CDS             complement(<1..822)
                     /locus_tag="VHR64_01655"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX3301554.1"
                     /translation="MFRLVAVLGLLLISGIISIAQTPPESVGAIQATPTASGDLVFEP
                     VADARVEEATPGSNHGGSSKLRADGGKDPAVESYLMFSVSNVSGPIGRAVLRVFATSD
                     TKAGLDAYVTDSSWAEMEITWDNRPAHAADPVASSGQVATNTWVEFDVTAAVTGDGTY
                     SFVLATSSSDGLNFSAREAGETAPQLVITPGNRTSTPIAGASPAGSPVSGDTATVLAA
                     GDVTSCSSKGSKATGELISSRPGEVLALGDLAYPSGAADTFKHCYDPVWGSFKDRT"
     gene            complement(973..3300)
                     /locus_tag="VHR64_01660"
     CDS             complement(973..3300)
                     /locus_tag="VHR64_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3301555.1"
                     /translation="MANTRGSFGDILRQYRTMAGMSQEALAERAGLTANAIGTLERGE
                     RRRPHPHTIRSLADALALTDEQRAALIAATGQQVAPQPLATSDLDPARPSALPAPPTP
                     LVGRDEVVAEISTILCRPEVRLVTLTGIGGVGKTRLAIEVARSTTADRPDGVVFVPLA
                     PILDPNLLIPTIGRALGLQETRAEMLRGQIFSYLRRRRILLVLDNMEHLLEAVGEIAD
                     LLAATEAPVVLVTSRRPLQIRGEVDIPVEPLSIELGSGTAIDLFAARAQDVNPSFRLT
                     ADNAGTVTAICRQLGGLPLAIELAAAWMRTLTPADLQSRLNHVLTVEGARDLPTRQRT
                     MRATLDWSHGLLSDAERLLFRRLAVFSGGFTLEAIESICPDESHPTAEVLASLSGLIE
                     HSLVKAQAGTTPRRYGMLEPVRQYAGEHLAASGEGAALRRRHADYYLALAAEAETGLI
                     GADQARWLALFDAEHPNVRAALRWLTDTGEHEQLVDLFWRTTQFFVTRGHFNEGARWL
                     EPVLAHDHEMSDLSRVRARFSVAYLITGQGRLEEAIQHCADGADIAQAAGEYRLAAMC
                     HGLAAGLGTYLGDAEVTRPHAEAAGAWYDANPIEWPVLGPQVYRAGRAMEQGDFAGAI
                     DVLEAAEAALRGAGYWWHLMHILNFRSQVAIAQGDAWQAARTCRDSIEVGNQIGVTLT
                     LPDTLAILGLALSQIGEAEQAARMFGAVEALRERLGDLLIVSSRREMYEQALARITDE
                     IGDEAMADAWQEGRIAPLDDVIADSLAPFAAITSA"
     gene            complement(3513..3647)
                     /locus_tag="VHR64_01665"
     CDS             complement(3513..3647)
                     /locus_tag="VHR64_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301556.1"
                     /translation="MTNSTHEDESRTAGLALASAIAICELIMLTLVICVAMGIGPLAG
                     "
     gene            3914..4513
                     /locus_tag="VHR64_01670"
     CDS             3914..4513
                     /locus_tag="VHR64_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3301557.1"
                     /translation="MARPGDIIEHPITRERLTFLETSAQTGGSSVRYQLEVAPGGFAT
                     APHVHPWIEERFEIISGEWLFVIEGVEQRLGPGDSALIPARQAHSWRNGGDEIGVAIV
                     ECRPALKTEEFFETFFGLAQDGLVDPTSGLPTLPWLAMMVVDYHNDFAHPAEPPLPVI
                     IEAMRPIADEARRQGLRVPYPYPYPDRSSDIVRGRGPAA"
     gene            complement(4522..4893)
                     /locus_tag="VHR64_01675"
     CDS             complement(4522..4893)
                     /locus_tag="VHR64_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301558.1"
                     /translation="MQTVRKTLRQIVAEAAEASYEARRVRSGGKPWSERSDEDRRGYE
                     KAVAAAFEFLPTIGYAVKPDGPAGVIPGMVLLPLEDARALWQAFTRTDVPREATLRAY
                     SSLQAAIAEAEEHDIPELDPE"
     gene            5089..5961
                     /locus_tag="VHR64_01680"
     CDS             5089..5961
                     /locus_tag="VHR64_01680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3301559.1"
                     /translation="MTTIYELPSSQFELARPVLGSPPADFAYIDAGLTGMSPARIFVD
                     DQQRPAAALMTRSYEYFIGGERSASLAEFIRDAPIEVGVWADFYGFVAVDPAWNDHLR
                     QLHPDLETIGRRSFRFDPARLDLVRGWQERVPLGLRVVPLTVELAEMADREMPEVIGK
                     IWAGYDLYAKYGFGALMLDGDAPVSVCCAFGVGGGEANLGVATIPAYHRRGLALTCSH
                     ACIEMTVNRGLIPTWDCDAPNVASGDLARAIGFTEHDSFTELAFPDRAKPNQSTGAWS
                     SEQTEHGVIAWTKR"
     gene            complement(6017..6649)
                     /locus_tag="VHR64_01685"
     CDS             complement(6017..6649)
                     /locus_tag="VHR64_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX3301560.1"
                     /translation="MPITIDVVQDTVCPWCRIGKKNLDDALKNFDGEAEVRFHPFFLR
                     PEMPAAGRDFREHMRSIKGDDDIDPLLLRVSNIGLQAGLTFNWDKVKRMPNTLLSHAL
                     YLSAPTDEQGRVLDAIYQAYFIDGRDIGKKQTLIEIGEDLPVDTSKLDDEAFLDEIAG
                     RAEAASAQGISGVPFFIINGQVGISGAQPPSVLLEAMEEASSRAVEQASS"
     gene            complement(6861..7778)
                     /locus_tag="VHR64_01690"
     CDS             complement(6861..7778)
                     /locus_tag="VHR64_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3301561.1"
                     /translation="MSERPIKIGIMLPESEFEMAGQTAGWSDFSAMAQATEECGFDSI
                     WFADHLQLKLPDGRDQGAWECWSILSAIAATTSRIELGPFVTATSYRNPAMLARIAET
                     VDEISGGRLIFGIGAGWAEHEYRVHGWPYDHRASRFEEALTIITNLIRTGQVDFTGEY
                     YTIREALLRPRGPRPGGMPIMIGTFSGERMMQLAAQHADHWNIWANAFGNRPETLEPL
                     LARMDEYCRAVDRDPASLTRSASVLVDFDDPYGRPGQVVPSLTGTPRELADAYLRYAE
                     AGVDHLQLYPDPCTVDGIKHCAEALELVT"
BASE COUNT         1268 a         2583 c         2642 g         1390 t
ORIGIN      
        1 ggttcgatcc ttgaagctac cccagaccgg gtcgtagcag tgtttgaacg tgtccgccgc
       61 ccccgagggg taggccagat cgccgagcgc cagaacctca cccggccggg atgagataag
      121 ctcgccggtc gccttcgagc cttttgagct gcaggaggtg acgtcaccgg ccgcgagtac
      181 ggtagccgtg tcgccgctaa ccggtgagcc ggctggcgat gcccctgcta ttggagttga
      241 tgtgcggttg ccaggagtaa tgaccagttg aggcgccgtc tcccctgctt ctcgggcgct
      301 gaagttcagg ccgtcggagg agctagtcgc gagcacaaag ctgtaggtgc cgtcgccggt
      361 gacggctgcc gtcacgtcga actcgaccca cgtgttggtc gccacctggc ccgaactggc
      421 gaccgggtca gcggcgtggg ccgggcgatt gtcccaggtg atctccatct ctgcccagct
      481 cgaatcggtg acataggcgt ccagtccggc tttggtgtcg gatgtcgcga atacgcggag
      541 gacggcacgc ccgatcgggc cggacacgtt ggagaccgag aacatcaaat agctctcgac
      601 ggccgggtcc ttcccgccgt cagcacgaag cttcgatgat ccgccgtggt tgctgccggg
      661 tgttgcttct tcaacccgag cgtcggccac cggctcgaac acgagatcac ccgaggcggt
      721 tggagtcgcc tgaatcgctc ctacggactc tggcggagtc tgggcgatcg agatgatgcc
      781 gctgatcagg agtagtccga ggacagcgac gagccgaaac attattcgag tccttcctgg
      841 cgcgggtacc ggatcacatg gaagcgagag tcagcgggga ctggaatctg gcttgggtgt
      901 aggcggcgcc tggtgattca gcatagcggt gtaacaagtc gtacgctcat tctatgcgta
      961 cgtcgactgc gatcaagcgg acgtaatcgc cgcgaagggc gcgagggaat cggcgatcac
     1021 gtcgtcgagc ggcgcgatgc ggccctcctg ccaggcgtcg gccatcgcct cgtcgccgat
     1081 ctcgtccgtg atccgggcca gcgcctgctc gtacatctcc cgccgggagg agacgatcag
     1141 caggtcgccg agccgctccc gcaatgcctc gaccgcgccg aacattcgcg ccgcctgctc
     1201 cgcttcgcca atctgcgaca gggccagccc gaggatcgcc agcgtgtcgg gcagggtcag
     1261 ggtcacgccg atctgattgc cgacctcgat cgagtcccgg catgttcgcg ccgcctgcca
     1321 ggcatcaccc tgcgcaatcg cgacctggct gcggaagttg aggatatgca tcaggtgcca
     1381 ccagtacccg gctccccgca gggctgcctc ggccgcttcg agcacgtcga tcgcccccgc
     1441 gaagtcgccc tgctccatgg cccggccggc gcggtagacc tgggggccga ggaccggcca
     1501 ctcgatcgga ttggcgtcgt accaggcccc ggcggcttcg gcatgaggac gggtgacctc
     1561 ggcatcgccg agataggtgc caagcccggc tgccaacccg tggcacatcg cggccagccg
     1621 gtactcacca gccgcctgcg cgatgtcggc gccatctgca cagtgctgga tcgcctcctc
     1681 caggcgaccc tgaccggtga ttaggtaggc gaccgagaac cgtgcccgga cccggctgag
     1741 atcgctcatc tcgtggtcat gtgcgagaac cggctcgagc cagcgcgcgc cctcgttgaa
     1801 atgaccgcgg gtgacgaaga actgcgtcgt tcgccaaaac aggtccacca gttgctcgtg
     1861 ctcgccggtg tcggtcagcc agcgcagcgc cgcccggaca ttcggatgct cggcgtcgaa
     1921 cagggcgagc cagcgggctt gatcggcgcc gatcagtccg gtttccgctt ctgccgccag
     1981 ggccaggtag tagtctgcat ggcgcctgcg aagagcagca ccttcgccac ttgccgccaa
     2041 gtgctcgccg gcgtactggc gaaccggctc cagcatcccg tagcggcgcg gcgtcgtccc
     2101 tgcctgggcc ttgaccagcg agtgctcgat cagcccactg agcgaggcca gcacctcagc
     2161 ggtcgggtgg gactcgtccg ggcagatcga ctcgattgcc tcgagcgtga agccgccgga
     2221 gaagacggcc agccggcgga agaggagccg ctccgcatcg ctgagcaggc cgtggctcca
     2281 gtcgagcgtc gcccgcatcg tgcgctggcg ggtgggcaaa tcgcgcgcgc cctcgacagt
     2341 aaggacgtga ttgaggcggc tctgcaggtc ggccggggta agggtccgca tccaggcggc
     2401 cgccaactcg atcgcgagcg gcaatccgcc gagctgccgg cagatcgcgg tcacggtccc
     2461 ggcgttgtcg gcggtcagcc ggaagctcgg gttgacgtcc tgggcacggg cggcaaagag
     2521 gtcgatcgcc gtcccggacc cgagctcgat cgacagcggc tcgacgggta tgtcgacctc
     2581 accccggatc tggagcgggc ggcgactggt gaccagcacc accggcgcct cggtcgcggc
     2641 caggagatcg gcgatctccc cgaccgcctc cagcaaatgc tccatgttgt cgagcacgag
     2701 caggattcga cggcggcgca ggtaactgaa gatctgcccc cgcagcatct cggcgcgcgt
     2761 ctcctgcagc ccgagcgcgc ggccgatcgt cgggatcagc aggttcggat cgaggatcgg
     2821 cgcaagcgga acgaagacga cgccgtccgg ccggtcggcg gtcgtcgagc gggcgacctc
     2881 gatggcgagc cgggtcttgc cgaccccgcc gatccccgtc agcgtcacga gtcggacctc
     2941 cgggcggcac aggatggtgc tgatttccgc gacgacctca tcccggccga ccagcggtgt
     3001 cggtggcgcc gggagcgcgc tgggtcttgc cgggtccagg tccgatgtcg cgagcggctg
     3061 cggcgcgacc tgctggccgg tcgccgcgat cagggcggcg cgctgctcgt ctgtcaacgc
     3121 gagggcatcg gccagggagc gaatcgtgtg cgggtgggga cgtcgccgct cgccgcgttc
     3181 gagcgtgccg atcgcgttgg cggtcaaccc ggcgcgctcg gccagcgcct cctggctcat
     3241 cccggccatg gtgcggtact ggcgcaggat gtcgccaaat gatccgcgcg tgttggccat
     3301 ggagccgcgt ccccgggtgt cggacaggta aaaagcgctc caatggtagc aattgacgca
     3361 ttcccttgcg gcaaaagacg tagcgcgcgg ccgccgtgct gcgctgtggc cgtgggccac
     3421 ggggatctcg tcggggaatc cgtaccctaa tcgtggccgg tgtcttggaa tggaatgcgc
     3481 ggctcagcgg ccgcgcgcta cgttttccga cgttaccccg ccaacgggcc gatgcccatt
     3541 gcgacgcaga tcacgagcgt cagcatgatc agctcgcaga tggcgattgc gctcgccaga
     3601 gccaggcctg ctgtccggct ctcgtcctcg tgcgtggaat tggtcatcgt cgtctccctt
     3661 cgtgacaccc gggcgggaaa tccctcgccc ggtctgtgcc tgattggagc catcctgcgc
     3721 ttggtgcctt gggggaacct gtgaatcgtc cgggaatcga ctgagatcca ggatccagtg
     3781 atttgttggg gaactgtggg gaggccgtgg gtggtcgggg catccggccg gcgacagagt
     3841 agatatgccg gcgggaccgg cccggatgga ccgggaacag cgtccggcaa agctcagcat
     3901 cggaggcagc gcgatggcac ggccaggcga catcatcgaa catcccatca cgcgggaacg
     3961 actcaccttc cttgaaacct cggcccagac tggtggctcc tctgtccgct accagctcga
     4021 ggtcgctccc ggcggcttcg cgaccgcgcc ccatgttcac ccctggatcg aggagcgatt
     4081 cgagatcatt agcggcgagt ggctcttcgt gatcgagggc gtcgagcagc ggctgggtcc
     4141 gggcgactca gcgctcatcc ccgcgcgtca ggctcattcc tggcggaatg gcggcgatga
     4201 gatcggcgtg gcgattgtcg aatgccgacc ggcactcaag accgaggagt tcttcgagac
     4261 ctttttcggc ctggcccagg acggcctggt cgatccgacc tccggtctgc cgactctgcc
     4321 gtggctggcg atgatggtcg tcgactatca caacgacttc gcccacccgg ccgagccgcc
     4381 gctcccggtc atcatcgaag cgatgcggcc gatcgccgat gaagcccgga ggcaggggtt
     4441 gcgagtcccc tatccctacc cgtacccgga tcggtcgtcc gacatcgtgc ggggtcgtgg
     4501 tccggccgcc tgagacctgc cctattccgg atccagctcc gggatgtcgt gctcctcggc
     4561 ttcggcgatg gctgcctgca gggaggagta ggcgcgcagg gtggcttcgc gcgggacgtc
     4621 ggtccgcgtg aacgcctgcc acagcgcacg ggcatcctcc agcggcagga gcaccatgcc
     4681 ggggatcact cccgccggtc cgtccggttt gaccgcgtag ccgatcgtag gcaggaactc
     4741 gaatgcggcg gcgactgcct tctcgtagcc gcgccgatcc tcgtcggatc gctcgctcca
     4801 tggcttgccg cccgacctga cccggcgcgc ctcatacgag gcctcggccg cttctgccac
     4861 aatttgccgc aacgtctttc gtactgtttg catgcatcct gttcccggtg actgtttccg
     4921 gcatagattg gcgcgctcga tctgcagcgt gcccggtgag gttcccagcg cgcaccgtta
     4981 tgatcatcgc agcacgggtg cagatcgacc atgcattgag cgggaaggaa gtaggggctt
     5041 gtacgccaca gttcgtacct ggcgcctcgc cacaatgagt cggcccgcat gacgacgatc
     5101 tatgagttgc cgtcatccca attcgagttg gcccggccgg tgctgggcag tccgccggcg
     5161 gatttcgcat acatcgatgc cgggttgacc gggatgagcc cggcgcggat cttcgtcgac
     5221 gatcagcaac ggccggctgc cgccttgatg acgcgcagtt acgaatattt catcggcgga
     5281 gagcgttcgg catcgctggc cgagttcatc cgggatgcgc cgattgaggt cggtgtctgg
     5341 gccgatttct acggcttcgt ggcggtcgat ccggcctgga acgaccatct ccgccaactg
     5401 catcctgacc tcgagacgat cggccggcgc tcgttccgct tcgatccggc gagactcgac
     5461 ctggtgcgcg gctggcagga gcgcgtgccg ctggggctcc gtgtcgtgcc gctgaccgtc
     5521 gagctggctg aaatggcgga tcgggagatg cccgaggtga tcggcaagat ctgggcaggc
     5581 tacgacctgt acgcgaaata cgggttcggc gcgttgatgc tcgatggcga tgcgccggtc
     5641 tcggtctgct gcgcgtttgg agttggcggc ggcgaggcaa acctgggtgt cgcgacgatc
     5701 cccgcctacc accggcgggg cctcgccctg acgtgcagcc atgcctgcat cgagatgacc
     5761 gtcaatcgcg gcctgatccc gacctgggac tgcgatgcgc cgaacgtcgc ttcaggcgat
     5821 ctggcccgcg cgatcggctt caccgagcac gactccttca ccgagctcgc cttccccgac
     5881 cgcgccaagc cgaatcagtc caccggtgca tggtcgagcg agcagaccga gcacggcgtc
     5941 attgcctgga cgaagcggta accctcaccc ctgacccctc tcccgccgag aaaaaggggc
     6001 gagagagggg aggtccttaa ctgctcgcct gctcgaccgc tcgactgctc gcctcctcca
     6061 tcgcttcgag gaggacgctt ggcggttggg cgccggagat tccgacctgg ccgttgatga
     6121 tgaagaaggg gacaccgctg atgccctggg cgctggcggc ttcggcccgg ccggcgatct
     6181 cgtccagaaa tgcctcatcg tccagcttgc ttgtgtccac cggcaggtcc tcgccgatct
     6241 cgatcagcgt ctgcttcttg ccgatgtcac gcccgtcgat gaaataggcc tggtaaatgg
     6301 cgtcgagtac tcggccctgc tcatcagtcg gggcggaaag atagagcgcg tgcgagagga
     6361 gcgtgttcgg catgcgcttg accttgtccc agttgaaggt caggcccgcc tgcaagccga
     6421 tgttggagac gcgaagcagc aacggatcga tatcgtcgtc acccttgatc gaccgcatgt
     6481 gctcgcggaa gtcgcgtccg gctgccggca tctccggccg caggaagaac gggtggaacc
     6541 gcacctcggc ctcgccgtcg aagttcttga gcgcgtcatc caggttcttc ttgccgatcc
     6601 ggcaccacgg gcaaaccgtg tcctgaacga catcgatggt gattggcaat gctgtccctc
     6661 tgcctaatgg ggtgaatcga tagagaaacc aaatcgtagc gcggggttac cacgccccgc
     6721 ccgggtttga tgacgatcgg tctatcgcgg gaatcaatcc cgtagacggc cgggtttcat
     6781 caaccgggcg ttggggatgc gccaagccgg gcctgttcca ctctccggct ctctcctcaa
     6841 ggaacgaggg caaccccgcg ttacgttacg agttccaatg cctcggcgca gtgtttgatg
     6901 ccgtcgaccg tgcatggatc cgggtacaac tgtaggtggt cgacgcctgc ctcggcgtag
     6961 cgcaggtagg catcggccaa ctcccggggt gtcccggtca gcgacggcac gacctggcct
     7021 ggccggccgt acgggtcgtc gaaatcgacc agaaccgatg cgctccgggt caagctcgcc
     7081 ggatcgcgat cgaccgcgcg gcaatactcg tccatcctgg cgagcagcgg ctcgagcgtc
     7141 tcgggccgat tgccgaaggc gttggcccag atattccagt gatcggcatg ctgcgcagcc
     7201 agctgcatca tccgctcgcc gctgaaggtg ccgatcatga tcggcatccc gcccggtcgc
     7261 ggaccgcgcg gccgcagcag cgcctcccgg atcgtgtagt actcgcccgt gaaatcgacc
     7321 tggccggtcc ggatcaggtt ggtgatgatg gtcagcgcct cctcgaagcg gctggcccga
     7381 tgatcgtagg gccagccgtg gacgcgatat tcgtgctctg cccagccggc gccgatcccg
     7441 aagatcaggc gaccgccgct gatctcgtcg accgtctcgg cgatccgggc cagcatggcc
     7501 gggttgcggt aggaggtggc ggtcacgaac gggccgagct cgatccggct cgtcgtcgcg
     7561 gcgattgccg aaaggatcga ccagcactcc cacgcgccct gatcccgccc gtccggcaat
     7621 ttgagctgca ggtgatcggc gaaccagatc gagtcgaagc cgcactcctc ggtcgcctgc
     7681 gccatcgccg agaaatcgga ccagcctgcg gtctggccgg ccatctcgaa ctcggactcg
     7741 ggcagcatga tcccgatctt gatcggccgc tcgctcatgc cggcacgccg gtcttgtcga
     7801 ggatctcgat gaccttcgcg aagccctcga tcgactgcgg cgtgcagggg tcgagcccgg
     7861 cgacatagtg gcggaagccc aga
//
LOCUS       DATAOZ010000057         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5551653, whole genome shotgun sequence.
ACCESSION   DATAOZ010000057 DATAOZ010000000
VERSION     DATAOZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5551653"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..734
                     /locus_tag="VHR64_01695"
     CDS             153..734
                     /locus_tag="VHR64_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301562.1"
                     /translation="MRQGNAAWGGQSIRAVRLPILLLAVVVLALIAVVAYAVQRELTK
                     PQVSEPAPVTAPAAQRALSAEEEDYAAALWPIHSEVKLAAVEMTFAGIAYKMGEQDAK
                     TLETKLRPLADTFETAMKRARALKVPASMTQMQDRYVGALALYDKAAAEMISATHDGK
                     AEHLVAGQQMSLNASEDILRIGDVLWPGEYKPH"
     gene            814..>1584
                     /locus_tag="VHR64_01700"
     CDS             814..>1584
                     /locus_tag="VHR64_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3301563.1"
                     /translation="MKNLVAVLVAVGATALGTTGSALAQTETNCSVWLVESSRFMKVP
                     FGSFMPDRAFVPGSTKPTNNIPTVDLPVNVGVIKCGDELILYDTGWKQQDYLKMTGSD
                     HWAPLPDQLKQLGFSADLVTKIVIGHGHWDHAGQIEDFPNAILYVQREELRGIEWALS
                     YPNQHIKAINSDPGGCFRTPACGYMPLTMQNIYGKVLAGKAVIVDGEMEILPGIKIHP
                     AFRAHTAGSQLLEVPTSIGKLVFGSDAYSSWEGIRDWMP"
BASE COUNT          295 a          488 c          540 g          261 t
ORIGIN      
        1 tagggtcgcc tccggattgc tcagcttcgg gtttggcctg ttcctggttt acgagatcgg
       61 cttcatggat ggcgggctgt tcactgcgag cccgacctgg acgcctcact aaggctcccc
      121 gattgacggg cagcggggga cggagctatc aaatgcggca agggaacgcc gcttggggag
      181 gccagtcgat ccgggccgta cggttgccga ttctgctgct ggccgtggtc gtgctcgcgc
      241 tgatcgcggt ggtcgcctac gcggtccagc gggaactcac caagccgcag gtgagcgaac
      301 ccgctccggt caccgcaccg gcggcgcaac gagcgctgag cgccgaggaa gaggactacg
      361 cggcggcgct gtggccgatc cacagcgagg tgaagcttgc cgcggtcgag atgaccttcg
      421 cggggatcgc ctacaagatg ggggagcagg atgcgaagac cctggagacc aagctcaggc
      481 cgctcgccga caccttcgag acggccatga agcgggcccg ggcgctgaag gtccccgcct
      541 cgatgaccca aatgcaggat cgctacgtcg gcgcgctcgc gctgtatgac aaagcggcgg
      601 cggaaatgat cagcgccacg cacgacggca aggccgagca tctcgtcgcc ggccagcaga
      661 tgagcctgaa tgcgtctgag gacatcctgc ggatcggcga cgtgctctgg ccgggcgagt
      721 acaagccgca ttgaattttc taccggcggc cgggagaccg gcggccgcgc tttcggcgac
      781 tggtgtgaag tggcgatcgg ggaggatcag ccgatgaaaa atctggttgc agtcttggtg
      841 gcggtcggtg cgaccgcgtt ggggaccacg ggcagcgcgc tcgcccagac ggagaccaac
      901 tgctcggtct ggctggtcga gagcagccgc ttcatgaagg tgccgttcgg gtcgttcatg
      961 cccgaccggg cgttcgtgcc cggcagcacg aagccgacca acaacatccc gacggtggat
     1021 ctgccggtca acgttggcgt catcaagtgc ggcgacgagt tgatactcta cgataccggc
     1081 tggaaacagc aggactatct caagatgacc ggctccgacc actgggcgcc gttgccggat
     1141 cagctaaagc agctcggctt cagcgccgac ctggtcacca agatcgtgat cggtcacggg
     1201 cactgggacc atgccggcca gatcgaggac ttcccgaacg cgatcctgta cgtgcaacgt
     1261 gaggagctgc gcgggatcga atgggccctc agctacccga accagcatat caaggcgatc
     1321 aacagcgatc cgggcggctg cttccgcacc cctgcgtgcg gttacatgcc gctgacaatg
     1381 cagaacatct atggcaaggt cctggccggc aaagcggtga tcgtagacgg cgagatggag
     1441 atcctgcccg ggatcaagat ccatccggcg ttccgggccc acaccgccgg ctcgcagctg
     1501 ctcgaggtgc cgaccagcat cggcaagctc gtgttcggct cggatgccta ctcctcctgg
     1561 gagggcatcc gcgactggat gccg
//
LOCUS       DATAOZ010000058         5891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4499458, whole genome shotgun sequence.
ACCESSION   DATAOZ010000058 DATAOZ010000000
VERSION     DATAOZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4499458"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VHR64_01705"
     CDS             <1..652
                     /locus_tag="VHR64_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3301564.1"
                     /translation="CDISGSMDRYSRLLLQFVHALEHGLDSVEVFVFSTRLTRITREL
                     RKRNVDAAIEHVVKSVDDWSGGTRIGEAIKDFNFKWGRRVLRSGATVVMISDGWDRGD
                     PKLLSKEMARLQRSCRRLIWLNPLLGAPGYQPLTQGIRAALPYVDYFLPIHNLKSMEA
                     LAKMLGEVDDQPPVRRQSTVAGTAAEQLPGIARIGNRGELTIEIDPKYLARVNVRR"
     gene            657..986
                     /locus_tag="VHR64_01710"
     CDS             657..986
                     /locus_tag="VHR64_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX3301565.1"
                     /translation="MREFLPEVDAWRARGDRIAIATVVKVEGSAPRPVGATMAISSTG
                     DLAGSVSGGCVETAVFDIAQEVLKNNQPTLVHYGITDEMAWDVGLACGGAIDVYVEPL
                     DLPVEAS"
     gene            983..1771
                     /locus_tag="VHR64_01715"
     CDS             983..1771
                     /locus_tag="VHR64_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX3301566.1"
                     /translation="MSQLTTADLYDRMREAAERYEPAALVTIVNGPGAGRKLAIVGSE
                     QIGTLGNERLDQHAVEHANALMDAEKSETVPTEGPEGSVDLFYDVYPAPPTLIIFGAV
                     HAAQPLTKIAKMLGYRVLISDARAKLATEERFPEADEVIVAWPDEALATYRIGRNTYL
                     AILTHDPKFDEPALIGALDTKAPYIGAVGSRKTNRDRRERLRNAGVSDESLSRIRGPI
                     GLDIGASSPEEMAISIIAEIIAVRKGRVGGPLTEATGSIRGEAP"
     gene            1755..2372
                     /locus_tag="VHR64_01720"
     CDS             1755..2372
                     /locus_tag="VHR64_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX3301567.1"
                     /translation="MAKPPDQLKVAGIILAAGQSTRLGRPKQLLDICGKTAIRRITEA
                     ALAAPLDSVIVVLGNAAAEIERELTGLDVLTVLNPDFAEGQSTSMRAGLRAIPDDTDA
                     ALFLLGDQPAMSPKTIELVFDTYRISGAAIVQARFRGVMGHPVLFDRSLFSALESVTG
                     DRGARQVIARHPELVQYADLDRDAPLDIDTEADYERVLAQFCSST"
     gene            complement(2416..3765)
                     /locus_tag="VHR64_01725"
     CDS             complement(2416..3765)
                     /locus_tag="VHR64_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3301568.1"
                     /translation="MIADFEAHYASSREMFERAQQSIAGGIAHDGRHQKPFPLYVSRA
                     DGAYKYDIEDRRLIDYAMGHGALILGHGDPDVRAAMHAQIDKGTHYGSGHEGEIAWAE
                     QIRKLIPSAERVRFVASGTEANLLAMRLARSYTGKNTILKFEGHFHGWSDYVIKSEKP
                     PFESPKVAGVPDDVLRTVAAVPANNIEMLTERLSQGDVAAILVEPSGASWGQIPLDAD
                     FLTKLRDVATESGAVLIFDEVITGFRWSPGGAQKRFGITPDMTTMAKIVAGGMPGGAV
                     AGQDEIMAFLEFKDEPGWAKRKVIHPGTFNANPLTAAAGLACLQKCADPAVQEHCDRL
                     ASHLRSGINVLLDKRGLPGCAWGASSAFHIILDETPKNRTAGDLAQPIGIHPETLKSS
                     AKAGRSGPLSLAMQLEGVDLFGGGGMLSVRHTDEDIAFTIDAFDRALDRLERDGYFA"
     gene            3897..>5891
                     /gene="priA"
                     /locus_tag="VHR64_01730"
     CDS             3897..>5891
                     /gene="priA"
                     /locus_tag="VHR64_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX3301569.1"
                     /translation="MNEDLHPRFAEVAVDGTLPGGDSPTLTYLVPPDCGPGVARGALV
                     WVPLRKKAALGVVVDVHDREPDFDVRPIYDIAPGDSLNRDQIELGMWLQRETASSLFG
                     CLSLMLPPGVTHSVTPWFEIRSRAPGKTKMQEKVLGLLGRNGSMSLDQLQQAMGSTLS
                     TVLDDLERTGQIARSYRSERRAARQRTERWWIAANGVDQAGLTGRQRELYEVIAKAGA
                     RGIRANEAIDLTGVSAAVTRKLVDSGAVTVDERPIVRRVEVDPPGTMPTLNPEQQHAW
                     LGIEAELRRRVGRPQVIFGVTGSGKTELYLRAIAATLRQGRQAIYLVPEIALTTQIAQ
                     RVKERFPGRVAVLHSGLAMGVRQEAWDQIASGERSVVVGARSALFAPVPELGLIVIDE
                     EHDPSYKQDIDPRYDARAAAQALADRHRAVVLLGSATPRTETLWRANSGEYDVYRLRK
                     RAVSTAPDLPPVQVVDLRMELQSGHTSLISRPLQDAIQQALLRGEQAMLLLNRRGMAT
                     VVLCRVCGRARICPNCSIPLVYHEDRGLLICHRCDHRERPTRECPHCEGPLDYFGAGT
                     QRVEAEAKRLFAGARVARWDQDVAKRSGGNASILQAIERREIDVVIGTQLIAKGLDLP
                     YVTTVGITNADVGLHFPDFRSGERTFQLITQMAGRAGRRTP"
BASE COUNT          989 a         1852 c         2042 g         1008 t
ORIGIN      
        1 ctgcgatatc tccggctcga tggatcgcta ttcgcggttg ctgcttcagt tcgtgcatgc
       61 gctcgaacac ggtctcgatt cggtcgaggt cttcgtcttc agcacgcgcc tgacccggat
      121 tacgcgagag ctgcgcaagc gcaacgtcga cgccgcgatc gagcacgtcg tcaagtcggt
      181 cgacgactgg tccggcggga cgcggatcgg cgaggcgatc aaggacttca acttcaagtg
      241 ggggcgccgc gtgctgcggt cgggcgcgac ggttgtgatg atctcggacg gatgggaccg
      301 gggcgacccg aagctgctct cgaaggagat ggcgcggctg cagcgatcgt gccggcggct
      361 gatctggctc aacccactcc tcggagcccc gggataccag ccgctgacgc aggggatccg
      421 ggccgcgctg ccctacgttg actacttcct gccgatccat aacctgaaga gcatggaggc
      481 gctggcgaag atgctcggcg aggtcgatga ccaaccgccg gtgcgacgcc agagcacggt
      541 cgccgggacc gccgcagagc agttgcccgg catcgcccgg atcggtaatc gcggcgagct
      601 gacgatcgag atcgatccca agtacctggc tcgcgtgaac gtacggaggt gacgtgatga
      661 gagagttcct gcccgaagtg gatgcctggc gcgcccgggg tgaccggatc gccatagcca
      721 cggtggtcaa ggttgaaggc tccgcgccgc gaccggtcgg cgcgacgatg gccatttcca
      781 gtactggcga cctggccggc tcggtctccg gcggttgcgt cgagacggcc gtctttgaca
      841 tcgcccagga agtcctcaag aacaaccagc cgacgctggt tcattacggc atcaccgacg
      901 agatggcgtg ggatgtcggg ctggcgtgcg gcggcgcgat cgacgtctac gtcgagccac
      961 tcgatctgcc ggtggaggcg tcgtgagcca actgacgacc gccgatctct acgatcggat
     1021 gcgcgaggcg gccgagcggt acgagccggc cgcgctggtg acgatcgtca atgggccggg
     1081 cgcgggccgg aagctggcga tcgtcggctc ggagcagatc ggcacactcg gcaatgagcg
     1141 attggatcag catgctgtcg agcatgccaa cgcgttgatg gatgccgaga agtcggaaac
     1201 ggtgccgacg gaagggccgg agggatcggt cgatctcttc tacgacgtct acccggcgcc
     1261 gccgacattg atcatcttcg gcgcggtcca cgccgcccag ccgttgacca aaatcgccaa
     1321 gatgcttggc tatcgggtgc tgatcagcga cgctcgcgcg aagctggcca ctgaggagcg
     1381 cttcccggaa gcggacgagg tgatcgtcgc ctggccggac gaggcgctgg cgacgtaccg
     1441 gatcggccgc aacacgtacc tcgcgatcct gacccatgat ccgaagttcg acgagccggc
     1501 cctgatcggc gcgctcgaca ccaaagcccc gtacatcggt gccgtcggca gccgtaagac
     1561 gaaccgggat cggcgggaac gacttcggaa tgcgggcgtc tccgatgagt cgctttcgcg
     1621 tattcgcggt ccgatcggac tcgatatcgg cgcgagctcg cccgaagaga tggcgataag
     1681 catcatcgcc gagatcatcg cggtccggaa ggggcgcgtg ggcggtccgc tgaccgaagc
     1741 caccggatcg atccgtggcg aagccccctg accaactgaa ggtcgcgggc atcatccttg
     1801 cggcaggtca gtcaacccgg ctgggtcgac ccaagcaatt gctcgatatc tgcggaaaga
     1861 cggcgatccg gcggatcacc gaagcggcgc tggctgctcc gcttgattca gtcatcgtcg
     1921 tgcttggaaa tgcagcggcc gagattgaac gggaactgac cggtctcgac gttctgaccg
     1981 tcctcaatcc ggactttgcc gagggacagt ccacctcgat gcgggccggg ctccgtgcga
     2041 tcccggatga caccgacgcc gcactattcc tgctcggcga ccagccggcg atgtcgccaa
     2101 agacaattga gctggttttc gatacgtaca ggatttcggg cgcggcgatc gtgcaggcac
     2161 gctttcgcgg tgtgatgggg catccggtcc tgttcgaccg atccctgttc agcgcgcttg
     2221 aatcagtgac cggtgaccgg ggagcgcgcc aggtcatcgc ccgtcacccc gagctcgtcc
     2281 agtacgccga cctggaccgg gacgcgcccc tcgacatcga taccgaagcc gactacgagc
     2341 gcgtactggc gcaattttgc agttcgacgt agcccggggc cgccgtgccc cgcgctacga
     2401 cttctgaggc acggttcagg cgaagtagcc gtctcgctcg agtcggtcga gggcgcggtc
     2461 gaacgcgtcg atcgtgaagg cgatgtcctc atcggtatgg cgaacgctga gcattccgcc
     2521 gccaccgaag agatcgactc cctccaactg cattgccagg gaaagcgggc ctgagcggcc
     2581 ggccttggcg cttgacttga gcgtctccgg gtggatcccg atcggctggg cgaggtcgcc
     2641 ggcggtccgg ttcttcggcg tctcgtcgag gatgatgtgg aatgccgaac tggcgcccca
     2701 cgcgcatcca ggcaaccccc gcttgtcgag caagacgttg atgccgctcc gcaaatgcga
     2761 ggcgagccgg tcgcaatgct cctggacggc cggatcggcg cacttctgga gacaggcgag
     2821 accggccgcg gccgtcagcg ggttggcatt gaaggtgccg ggatgaataa ccttgcgctt
     2881 cgcccaaccc ggctcgtcct tgaactcgag gaaggccata atctcgtcct ggccagcgac
     2941 cgcgccaccc ggcatgccgc cggccacgat cttggccatc gtggtcatgt ccggggtgat
     3001 cccgaaccgc ttctgcgcgc cgccaggaga ccagcggaag ccagtgatca cttcgtcgaa
     3061 gatcaggacc gcgccggact cggtggcgac atcgcgcagc ttggtcagga agtcagcatc
     3121 gagcgggatc tggccccacg acgcgccgct tggctcgacc aggatcgcgg cgacatcgcc
     3181 ctgcgagagc cgctcggtca gcatctcgat gttgttggcc gggacagccg cgacggtccg
     3241 cagcacgtcg tccggcacgc cggcaacctt cggcgactcg aagggcggct tctcgctctt
     3301 gatgacgtag tcgctccagc cgtggaagtg gccctcaaac ttgaggatcg tgttcttgcc
     3361 ggtgtaggag cgggcgagcc gcatcgccag cagattcgcc tcggtgccgg aagcgacgaa
     3421 gcggacgcgc tcggccgacg ggatcagctt gcggatctgc tccgcccagg cgatctcgcc
     3481 ctcatggccg gagccgtagt gggtcccctt gtcgatctgc gcgtgcatcg cggcgcggac
     3541 atccgggtcg ccgtggccga ggatcaatgc gccgtggccc atcgcgtagt cgatcaggcg
     3601 gcggtcttcg atgtcgtact tgtaggcacc gtcggcccgg ctgacgtaga gcgggaacgg
     3661 cttctggtgc cgcccgtcat gggcgatgcc gccggcgatg ctctgctgcg cgcgctcgaa
     3721 catctcccgt gacgacgcgt aatgcgcctc gaaatccgcg atcaactggg tcctggaagg
     3781 ggcttgaacc atcgatgggg ccttctttcc gccgtttgaa ctgagcgttc cgctgcccgg
     3841 aattataggg cggttgactc cccggcaaat tgacagtttg tcgtacgctt gttcgcatga
     3901 acgaggattt gcacccgcga ttcgccgagg tcgccgtcga cggaacgctg cccggcggcg
     3961 attccccgac cctgacctat ctcgtgccgc ccgattgcgg tccaggggtt gcgaggggcg
     4021 ctttggtctg ggtaccgctg cggaagaagg cggcgctcgg ggtggttgtc gatgtccacg
     4081 accgcgagcc ggatttcgat gtgcggccga tctatgacat cgcgcctggg gatagcctga
     4141 atcgcgatca gatcgagctt ggcatgtggc tgcagcggga gacggcgtcg agcctgttcg
     4201 gctgcctctc gctgatgctg ccgccgggcg tgacgcactc ggtcacgccc tggttcgaga
     4261 tcaggagccg tgcgccaggc aagacgaaga tgcaggagaa ggtcctcggg ctcctgggcc
     4321 ggaatggatc gatgtcgctc gatcagctcc agcaggcaat gggctcgacg ctttcgacgg
     4381 tgctcgacga ccttgaaagg accggacaga tcgcccggag ctaccggtcc gagcgtcgtg
     4441 ccgcgcggca acgcaccgag cgctggtgga tcgccgcgaa tggcgtcgat caagcgggtc
     4501 tgacgggccg gcagcgcgag ttatacgagg tgattgccaa ggctggggcc cgcggaatcc
     4561 gggccaacga agcgatcgat ctgacgggag tatcagcggc ggtgaccagg aagctggtcg
     4621 attccggcgc ggtaacggtc gatgagcggc cgatcgtgcg cagggtagag gttgatccgc
     4681 ccgggaccat gccgacgctc aatcccgagc agcagcatgc ctggctcggg atcgaggccg
     4741 agttgcggcg gcgggtggga cgtccgcagg tgatcttcgg cgtgaccggc agcggcaaga
     4801 ccgagctcta cctgcgcgcg atcgcggcga ccttgcggca gggtcggcag gcgatttacc
     4861 tcgtgccgga gattgcgctg acgacccaga ttgcgcagcg cgtcaaggag cgattcccag
     4921 gaagggtcgc ggtgctccac tcgggtttgg ccatgggtgt ccggcaggag gcgtgggacc
     4981 agatcgcgtc gggcgagcgc tccgtggtgg tcggcgcgcg ctcggctctg ttcgcgcctg
     5041 tacccgaact cggcctgatc gtgatcgatg aggagcacga tccgtcgtac aagcaggaca
     5101 tcgatccgcg ctacgacgct cgcgcggcgg cgcaagcatt ggccgatcgc caccgggccg
     5161 tcgtgctcct gggcagcgct acgccgcgca cggagacgct ctggcgcgcg aattcgggcg
     5221 agtacgacgt ctaccggctg cgcaaacgcg ccgtctcgac cgcgcccgat ttgccgcccg
     5281 tccaggtcgt tgacctccgc atggagcttc agtcagggca cacgtcgctg atcagccgtc
     5341 cgttgcagga tgcgatccag caggcactcc tgcgcggcga gcaggcaatg ctgctcctga
     5401 accggcgcgg catggcgacg gtcgtgctct gccgggtctg cgggcgggcg cggatttgcc
     5461 cgaattgctc gatcccgctg gtctaccacg aggatcgcgg cctgctgatc tgccaccgct
     5521 gcgatcaccg ggagcgaccg acccgagagt gcccgcactg cgaagggccg ctcgactact
     5581 ttggcgccgg cacgcagcgg gtcgaggcgg aggcgaagcg tctcttcgcc ggcgcgcgcg
     5641 tggcgagatg ggatcaggac gtcgcgaagc gcagcggtgg caatgcctcg attctgcagg
     5701 cgatcgagcg acgcgagatc gacgtcgtta tcggcacgca attgatcgcg aaggggctgg
     5761 atctgccgta cgtgacgacc gtcgggatca cgaacgccga cgtggggttg cacttcccgg
     5821 atttccgctc gggcgagcga acgttccagc tcatcacgca gatggccggc cgggcgggca
     5881 ggcggacgcc c
//
LOCUS       DATAOZ010000059         3464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6340871, whole genome shotgun sequence.
ACCESSION   DATAOZ010000059 DATAOZ010000000
VERSION     DATAOZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6340871"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VHR64_01735"
     CDS             <1..532
                     /locus_tag="VHR64_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3301570.1"
                     /translation="ATLGTAMEYARVLKPANMNCMAGKRLAEVPEDQQRATLIANLRA
                     AADQAAAEGILLVTEPLNPHDAPGFFLPRPSDGFAIVDEAKHPNLKVEYDIYHAQRTE
                     GEIVATIRNHIGAIGHIQIADNPGRNEPGTGELNWPFIFAAIDEAGYDTRIGLEYKPS
                     TERTDDSLSWMKEFAQ"
     gene            529..1425
                     /locus_tag="VHR64_01740"
     CDS             529..1425
                     /locus_tag="VHR64_01740"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR01505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEX3301571.1"
                     /translation="MTDAAAKTIGFIGTGIMGKPMVRNLVKAGYEVVIHNRSQRSVDE
                     LTAESDKLTAAGSPADVARAADTVILMLPDSPDVRDVVFGENGVLNEVRSGFLLIDMS
                     TIAPSTAIEVSNAVASAGGSVLDAPVSGGDKGAIAGTLSIMVGGTEADLKRAMPLFEV
                     MGKTIVHIGGPGAGQIAKACNQVMVAVNYAAASEALVLAERSGVDPGKVAQVLGGGLA
                     NSRVLEMRGETMIKGEFQPGFRVDLHRKDLAIALATGRERQSALPVTALVSQLYDALS
                     ATGGGQLDHSALISVIERMSGE"
     gene            1427..2194
                     /locus_tag="VHR64_01745"
     CDS             1427..2194
                     /locus_tag="VHR64_01745"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX3301572.1"
                     /translation="MAGFKEKLQSGAAVSGPIVSEIRTVGLIKALANAGHDFVWLDME
                     HAMFGWETIQTLTQYARAIGITPLVRATDLSYPLVARALDAGAIGVIVPRVDTREQVE
                     AAVQYAYYPPIGRRGAGGEGRYGYERRTPAEGVTEVNDTTVVVVQVETTEAVERIDEL
                     ASVDGLDVICVGPQDLSISLGIPGQYDNPTFVETVTHVMQRVTAAGKVCGMVERDARR
                     FQRWYDAGGRFFATNTDSNLIYLGAKADVDVVRGFSS"
     gene            complement(2191..3252)
                     /locus_tag="VHR64_01750"
     CDS             complement(2191..3252)
                     /locus_tag="VHR64_01750"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX3301573.1"
                     /translation="MAQFRIAVIPGDGIGSEVVPEGIKVLQRIAETSGGQISFAFESF
                     PWGSAYYHQHGRMMAENALSILERFDAIYFGAVGWPDVPDHVSLWGLRLAICQGFDQY
                     VCLRPVRLLPGIASPLRDASAERIDFVVVRENTEGEYAGLGGRRGSEDSEIALQTALF
                     TAHGCERIIRYAFDLAMTRPRRKVTSVTKSNAQQYGMTLWDDVFRRVAAEYPQVTTEQ
                     WLVDAMAARFVLKPESLDVVVGSNLFGDILSDLGSALAGSLGVAASANIDPERRKPSM
                     FEPVHGSAPDIAGQGIANPIGQIWSVALMLDHLGLPDEAKRIVRAIEHTTANGLTTPD
                     LGGTMSTVEVGDAIAAALD"
BASE COUNT          639 a         1087 c         1149 g          589 t
ORIGIN      
        1 cgcaacgctg ggcaccgcga tggaatatgc gcgcgtgttg aagccggcca atatgaactg
       61 catggccggc aagcgactcg ctgaagtccc ggaggaccag cagcgcgcaa ccttgatcgc
      121 caacctgcgt gccgcggctg accaggcggc cgctgagggc atcctgctgg tcacggagcc
      181 attgaatccg cacgacgcgc ccggcttctt cctgccccgg cccagcgatg gttttgccat
      241 cgtggacgag gcgaagcatc caaacctgaa ggtcgagtac gacatctatc acgcccagcg
      301 caccgagggc gagatcgtcg ccacgatccg caatcacatc ggcgcgatcg ggcatattca
      361 gattgccgac aatcccggtc ggaacgagcc cggcaccggc gagctcaact ggccattcat
      421 ctttgcggcc atcgacgagg ctggctacga cacccggatc gggctcgagt acaagcccag
      481 cactgagcgg accgatgatt cgctgagctg gatgaaggag ttcgcgcaat gacggacgca
      541 gcggcaaaga cgattggctt catcgggacc ggcatcatgg gcaagccgat ggtccgcaac
      601 ctggtcaagg ccggctacga ggtagtcatc cataaccgca gccagcgctc cgtcgatgag
      661 ttgaccgccg aatcggacaa gctcaccgcc gcaggatcgc cggcggacgt cgctcgcgcg
      721 gccgacacgg tgatccttat gctgccggat tcgcctgacg tgcgcgacgt cgtctttggc
      781 gagaatggcg tgctcaacga ggttcgatcc ggattcttgc tgatcgacat gagcacgatc
      841 gcgccgtcga ccgcgatcga agtcagcaac gccgtggcgt cggccggagg ctccgtgctc
      901 gatgcgccgg tcagcggcgg cgacaagggt gctatcgccg gcacgctcag catcatggtc
      961 ggcggcaccg aagccgacct caagcgggcg atgccgctct tcgaggtaat gggcaagact
     1021 atcgtgcaca tcggtggtcc gggcgccggc cagatcgcca aggcctgcaa ccaggtcatg
     1081 gtcgcggtca actacgccgc cgccagcgag gcgctggtcc tggctgagcg gtccggtgtt
     1141 gatccgggga aagtcgcgca ggtgctcggc ggtggcctgg ccaatagccg ggttctcgaa
     1201 atgcggggcg agaccatgat caagggcgag ttccagcccg ggttccgggt cgacctccac
     1261 cgcaaagacc ttgcgatcgc cctcgcgacc ggccgggagc gccagtcggc actgccggtc
     1321 accgcgctgg tcagccagct ttacgacgcg ctgtcggcga cgggcggcgg acaactggat
     1381 cattcggcgt tgatttcggt tattgaacgg atgtcggggg aataacgtgg ctgggttcaa
     1441 ggagaagttg cagagcggcg cggcggtcag cggaccgatc gtcagcgaga tccggacggt
     1501 tgggctgatc aaggcgctgg caaacgccgg gcacgatttt gtctggctgg acatggagca
     1561 cgccatgttc ggctgggaga cgatccagac cctcacgcag tacgcccggg caattggaat
     1621 cacacccctc gtgcgcgcga ccgatctgtc ctatccgctc gtggctcgag cgctggatgc
     1681 cggcgcgatc ggcgtcatcg tgccccgtgt cgatacgcgg gagcaggtcg aggcggcagt
     1741 gcagtatgcc tattacccgc cgatcggccg gaggggcgcc ggcggcgagg ggcgctatgg
     1801 atatgagcga cgcacacccg ctgagggtgt gaccgaggtc aacgacacaa ccgtcgtggt
     1861 cgtccaggtc gagacaaccg aagcggtcga gcggatcgac gaacttgcct cagtcgatgg
     1921 actcgatgtg atctgcgtgg gacctcagga cctttcgatc tcactcggca tccctgggca
     1981 gtacgacaac ccgacgttcg tcgagacggt cacgcacgtc atgcagcgcg tgaccgcggc
     2041 gggcaaggtc tgcgggatgg ttgagcggga cgcccgccgc ttccagcgtt ggtacgacgc
     2101 cggcggtcgc ttcttcgcga ccaacaccga tagcaatctg atctatctcg gcgccaaggc
     2161 cgacgtggac gtcgtccgcg gcttcagcag ctagtcaagc gctgccgcaa tcgcgtcacc
     2221 gacttcgacg gtactcatcg tgccgccgag gtcgggcgtg gtcagaccgt ttgcggttgt
     2281 gtgctcgatc gcgcggacga tccgcttcgc ctcgtccggt agcccgaggt gatcgagcat
     2341 gagagcgacg ctccagatct ggccgatcgg attggcgatt ccctgcccag cgatgtccgg
     2401 ggcggaaccg tgcaccggct cgaacatgct tggcttgcga cgctcggggt cgatattggc
     2461 actggccgcg acaccaagac tgccggccag cgcgctgccg aggtcggaga ggatatcgcc
     2521 gaagagattc gagccgacca cgacatcaag cgactcgggc ttgagcacga aacgagctgc
     2581 catcgcgtcc accagccact gctcggtcgt aacctgtgga tactcggccg cgacccggcg
     2641 aaagacatca tcccaaaggg tcatgccgta ttgctgggca ttgctcttgg tcacgctcgt
     2701 gaccttgcgc ctggggcgag tcatggcgag gtcgaaggcg tagcggatga tccgctcgca
     2761 gccgtgcgcg gtgaagagcg cggtctgcag cgcgatctcc gaatcttcgg agccgcgacg
     2821 tccaccgagg ccggcatatt cgccctcggt attctcacgg acgaccacga agtcgattcg
     2881 ctcggcgctg gcatctcgaa gtggactcgc gattcccggc aagagccgca ccggccgcaa
     2941 gcagacgtac tggtcgaacc cttggcaaat ggcaaggcgc agaccccaga gactgacgtg
     3001 gtcgggcacg tctggccagc cgacggcgcc gaaatagatc gcatcgaacc gctcgaggat
     3061 cgaaagggcg ttctcggcca tcatccggcc gtgctggtgg tagtaggcgc tgccccatgg
     3121 gaacgactcg aacgcgaaac tgatctgacc cccggatgtc tcggcgatac gctggaggac
     3181 cttgatgcct tccggcacga cctcactgcc gattccgtcg ccggggatga ctgcgatccg
     3241 aaactgcgcc attgcctgac tgctcctttg ccactcgttc ggcaatctag catgttgggc
     3301 gcggcgcaca ttggcacctg cgcgcgccag cggttaggtt gagcgcgaca gaaggagcgg
     3361 catgggcgac tcagcgcgga ccctttcgga agacatctac ctgcttggcg atctgctcgg
     3421 cgacgtgatc cagtcgcagg ccgggcaaga cgcgttcgcg ctcg
//
LOCUS       DATAOZ010000060         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7655576, whole genome shotgun sequence.
ACCESSION   DATAOZ010000060 DATAOZ010000000
VERSION     DATAOZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7655576"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..2555
                     /locus_tag="VHR64_01755"
     CDS             132..2555
                     /locus_tag="VHR64_01755"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX3301574.1"
                     /translation="MNFTTWPASGPLRHRRTAIATPETNRPWLQSLSVLIIIGLLSWL
                     GFAQLRTPAVVPASAPPTSFSAERAMAHLDVIASQSRAVGQPGHAAARDYLVAQLTAM
                     GLQPELQTTTSVLRFEGADSFGTGMVTNVIARIPGTANTGAIAINAHYDGGATGPAAG
                     DNGVGVAATLEVVRAVLAGPALANDLIIVFSDGEENGDLGAAAFNQLHLWAKDVRIAI
                     NFEAQGTGGPAMLYATSDDDGWLTGEYLSVAPDPSAYSMLPELVRALPGMRLACDLED
                     YLLNGAAGLGFVLADGTANYHTAQDKVANIDQGSMQQEGGNTLAAVRHFGTADLSTVP
                     TQPNRVYFTILPSVVVHYSSAWAMPIALLVTVMVGTMIVLGYRRRMLSIGGLGAGSVA
                     LIVGTLVTVLLVDLLWFVIKATNADLRVLLVAPYQARTIVLGLSLMAIAIMAAIYVVL
                     QRRIRAENLMAGGMLAWALLMWATVLAAPGASYVLTWPLLFAVLPLGWRILGRRRQLA
                     AELAVLAIALVPVALVMPGVSYQTIPLLHRVEFMMGLTGNIPVLGLWALFLAPLVGLL
                     IPHLNALVGGSTRPYRWLVPGGLAAVALALLVATSLTSGFDAEHPRPDNIAYELDANT
                     GMARFVSLDATLDSYTRQFFPADPTRADYEVQPGTTLRAWSVPTTAVALEGPVVEVLS
                     DRADGGGRVVTFRITSPRLAPELEATITGGGEITSAGINGRDLDLTDYAPARDGEFRF
                     SFVGMTADGVTVTMTMSGNAPISIALTERAYGLPVIPGLTVQQRTAAQMPAPAFPLDA
                     TIIRKTATI"
BASE COUNT          411 a          829 c          925 g          502 t
ORIGIN      
        1 cacccaggat cgcttctctg catctaccgt tcacctccac cgcaactcgc tcagtgccag
       61 cctcccgtgc ctcgcaccca agcggggcgc cgtgcggctc cccgggtgcc tgccccgggt
      121 ggaggtcgac gatgaatttc actacctggc cggcaagcgg tccgcttcgc catcgccgca
      181 ccgcgatcgc cacgccggag acgaaccgtc cgtggctgca aagcctgtcg gtcctgatca
      241 tcatcggatt gctgagttgg ctggggttcg cgcagctgcg gacgccggcc gtcgtgccgg
      301 cctcagcacc gccgacgtcg ttttcggcgg aacgggcaat ggcacatctc gatgtcatcg
      361 ccagccagag ccgggcggtc ggccagccgg gtcacgccgc cgcgcgcgat tacctggttg
      421 cacagttgac cgcgatgggg ctgcagccgg agctgcagac tacgacctcc gtgctccggt
      481 tcgagggcgc cgattcgttc gggaccggaa tggtgacgaa cgtgatcgcg cggattcccg
      541 gcacggcgaa taccggcgcg atcgcgatca acgcgcacta cgacggcggt gcgaccggtc
      601 ctgcggcggg tgacaacggc gtgggtgtgg ccgcgacgct ggaagtcgtg cgggcggtgc
      661 tggcgggacc agcactggca aatgacctga tcatcgtctt cagtgatggg gaggagaatg
      721 gagatctcgg cgcggctgcg ttcaatcaac tacacctgtg ggcgaaggat gtccggatcg
      781 cgatcaactt cgaggcgcag gggaccggtg gaccggcgat gctgtatgcg accagcgatg
      841 atgatggctg gctcaccggt gagtacctct cggtcgcgcc cgatccgagt gcctattcga
      901 tgctgccgga gctggtgcgg gcactgcccg ggatgcggct ggcctgtgat cttgaggact
      961 acctgctgaa cggcgcggcc ggattgggct tcgtgctggc ggatggtacg gcgaactatc
     1021 acaccgcgca ggacaaggtc gcgaacatcg accagggcag catgcagcaa gagggtggca
     1081 atacgctcgc cgccgtgcgc catttcggta cggcagatct ctcgaccgtc ccgacgcagc
     1141 cgaatcgggt ctacttcacg atattaccga gcgttgtcgt gcactacagc agtgcctggg
     1201 caatgccgat tgcgttgctg gtgaccgtga tggtcggcac gatgatcgtg ctcggttacc
     1261 gtcggcggat gctgagcatc ggcggccttg gagccggttc tgtcgctctc atcgtcggca
     1321 ccctcgtgac ggtcctcctc gtcgacctgc tctggttcgt gatcaaggcg acaaatgccg
     1381 atctgcgagt gctgctggta gctccatacc aagcccggac gatcgtgctc ggcttatcac
     1441 tgatggcgat cgcgatcatg gctgcaattt acgtcgtatt gcagcggcga atccgggcgg
     1501 agaacctgat ggcaggcggg atgctggcct gggcgctgct gatgtgggcg acagtgctgg
     1561 cagccccggg tgcgagctac gtcctgacgt ggccgttgct tttcgcggta ctgccgcttg
     1621 ggtggaggat tttggggcgg aggcgacagc tggcggccga actcgcggtg ctggcgatcg
     1681 cactggtgcc ggtggcgctg gtgatgcctg gcgtctccta ccagacgatc ccgctcctgc
     1741 accgggtcga gttcatgatg ggcctgacag ggaatatccc ggtgctcggg ttgtgggcgc
     1801 tcttcctggc gccgctggtt ggtctgctga tcccgcacct gaacgcgttg gtcggcggct
     1861 cgacccggcc gtatcggtgg ctcgtgcccg gtgggctggc ggctgtggcc ctggcgttac
     1921 tggtggcgac gagcctgacg tcggggttcg atgccgagca tccgcgaccg gacaacatcg
     1981 cctacgagct ggatgccaat acggggatgg cccggttcgt cagtctcgat gcgacgctcg
     2041 actcgtacac gcgccagttc ttcccggcgg acccgacgcg agccgactat gaggttcagc
     2101 ccggcacgac actacgagcc tggtccgtgc cgacgaccgc ggtcgcgctt gagggcccag
     2161 tcgtcgaggt gctcagcgac cgcgcggatg gcggcgggcg ggtcgtcaca ttccggatca
     2221 cctcgccgcg acttgcgccg gagctcgaag cgacgataac cggtggcggc gagatcacgt
     2281 cagccggaat caacggccgg gatctcgacc tgacggacta cgcgccggcc cgggacggtg
     2341 agtttcggtt ctcgttcgtg ggcatgaccg cggacggggt gacggtcacg atgactatga
     2401 gcggcaatgc cccgatctcg atcgcgctga ccgaacgtgc ctacggcctg ccggtgattc
     2461 ccggtctgac cgtgcagcag cgcacagcgg cccagatgcc ggccccggcg ttcccgctgg
     2521 acgccaccat cattcggaag accgcgacga tctgattcgc gtgtcgcgac gcggggtggg
     2581 cagatgtcca catcttttgt cacgaatttg ttgcgctgcg gcttctcgct gcaatccgga
     2641 tgggactggg tggagcgcat gctgacg
//
LOCUS       DATAOZ010000061         6569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4499793, whole genome shotgun sequence.
ACCESSION   DATAOZ010000061 DATAOZ010000000
VERSION     DATAOZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4499793"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VHR64_01760"
     CDS             complement(<1..1033)
                     /locus_tag="VHR64_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3301575.1"
                     /translation="MVKDRNELAELERAWRRSQPSGTRRDMLKWSAIAAGAVATMRTG
                     VAAASPARPSGAPAVLRQQDIEKDVTITVPLDPYGQVVTLDPHRSVNWGPFWAMFPNV
                     WGGLVRYDENAKVQLDLAESYTLSEDGKTYTFKIRSDAKFANGNKVTADHFVTSWKRA
                     LNPDQPSPMAYFMEPVRNYRKYLQKTSDAIGFKAVDESTVEITLSRAYSFFLSYLAAY
                     VWSVVDPAVVDKEGDEGFPLKDAGTGPWRFTEFDASSQVVMEPNSNHYGGVSPSIVKV
                     VWPFVTGPTAANTALNMYKQDKAISADVPISLKSPVERDPDLSKELVSIETSGSVRVL
                     AFDFKQEPFN"
     gene            complement(1374..3155)
                     /locus_tag="VHR64_01765"
     CDS             complement(1374..3155)
                     /locus_tag="VHR64_01765"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEX3301576.1"
                     /translation="MHIPSGDSRLRIGGLERAVRALMALSLLAAIGAGLLIQSPARAQ
                     EKGLLSTSSVTPADVVLYAEISLDTSSAQLQQFDKLLARLGSTESLIDAINKSATDPS
                     SDIDLTGAEVAVAVLPSALAEGASVGSEIVGGATSGESTSDLAQQVSSQAGNQGAVVI
                     IKPTDIASVEASMKKTASDEANNATVTYKGADITTYGDTEGGSTAYAVVGDYLFAGSA
                     ADDIKAFVDASQPGADTLANLDAFTKTSDLLPADRVAYAFLNGPVTIDAATKAAGDRS
                     MGDAIREVFATFSGYTGLVMTADDAGVRFESVIVPASGTANAKPAGSAVDLDFASRMP
                     ADTVVYASGFDLGRSMLMNALGLGIGLAFMGISSGATPTDQATPMPMSIDGIYEALAR
                     TFGINLKTALLDQMTGPYGFGVWGTDATDISGVNAVLLSGAKDSGIIGDSLGTISLLV
                     QAAGQGRINVTSKQLPGGSVNHVEFQSSGSTVAIDYGVIGDRLVLGLNDGAETALNSP
                     TDSLADADSFKTAMSYLPAEYQAVYFVDVAQLSATSAGASTTISGQDAISDFLGTPVA
                     ASAAQSFAAVTHIESGYAHTSAILVVP"
     gene            complement(3260..3394)
                     /locus_tag="VHR64_01770"
     CDS             complement(3260..3394)
                     /locus_tag="VHR64_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301577.1"
                     /translation="MDTIMGLVRAGITLAIIIIIIGLVAALFMIFFNLATGIDDRISE
                     "
     gene            complement(3500..6106)
                     /gene="clpB"
                     /locus_tag="VHR64_01775"
     CDS             complement(3500..6106)
                     /gene="clpB"
                     /locus_tag="VHR64_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX3301578.1"
                     /translation="MAANQRFTERAQEAIVEAQRNTQERKLSQLEPEAILAALLSQSD
                     GIVPQIVLKAQIDLPDLIAANERALSTLPSLQYSSEPTVSSATRKVLDTAQQEASQFG
                     DEYISTEHLLLGILATQSQAARLLAERGLTRDRVLELLKGIRGAQRVTTPNPEGTYAS
                     LEKYGRDLTAAAKDGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAEG
                     LAQRIVRGDVPEGLQDKRIIALDMGALIAGAKFRGEFEERLKAVLKEVQESDGEIILF
                     IDELHTVVGAGRTDGAMDAANLLKPALARGELHAIGATTLDEYRKHIEKDAALERRFQ
                     TVYVGEPTVEDTISILRGLRERYEVHHQVRILDSALVAAAVLSHRYIADRFLPDKAID
                     LVDEAAAKLRMEITSMPVELDEAERRIMQLEIEREALRKERDDASRARLEVLEGELAS
                     LKEQRDALRSRWDQERDAIQRISELRNQLDQTRTQIEQAQRSYDYNTAAELQYGKLVQ
                     IQDQIREQEEALTKLQASGKLLKEEVDADDIAEIVARWTGIPVSRLMEGEVEKLLHME
                     ERLHERVIGQEEAISAVANAIRRARAGLQDPNRPLGSFIFLGPTGVGKTELARALAEF
                     LFDTDEAIVRIDMSEYMERHAVARLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLFDEI
                     EKAHADVFNTLLQVLDDGRLTDSQGRVVDFKNTVIIMTSNLGSSSIVGLSTHDQEAMR
                     ERVMEAVRQHFRPEFLNRVDELVIFEPLGRDQIGEIVEIQLKRVSDRLSDRNIELQIT
                     PGAREWLGSRGYDPVYGARPLKRLIQREVLDSLAMKLLNGELHDGEVVTIDASPAGLT
                     FQAQLTEAPLVA"
     gene            complement(6170..>6569)
                     /locus_tag="VHR64_01780"
     CDS             complement(6170..>6569)
                     /locus_tag="VHR64_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3301579.1"
                     /translation="LFVISVAARLVKMHPQTLRKYEREGLIAPSRTQGNLRLYSDQDI
                     ERLRQVKYLVEDRGLNLAGVQMVLEITRRLRDIRAFTSDRGRDHGDAEGYTALTAELD
                     RMLRALGFVHEEQMTEIRRREGVVSESSRG"
BASE COUNT         1183 a         2133 c         1972 g         1281 t
ORIGIN      
        1 cgttgaatgg ttcctgcttg aagtcgaacg ccaggacgcg caccgatccg gacgtctcga
       61 tgctgacaag ctccttcgag aggtcaggat cgcgctcgac cggcgacttg agcgagattg
      121 gcacgtcggc cgaaatcgcc ttgtcctgct tgtacatgtt gagtgcggtg ttggctgcag
      181 tcgggccggt cacgaacggc cataccacct tgacgatcga cggagagacg ccgccgtagt
      241 ggttcgaatt cggctccatc accacctgcg atgaggcgtc gaactccgtg aatcgccaag
      301 gacctgttcc cgcatccttg agcgggaatc cctcatcacc ttccttgtcc acgaccgccg
      361 ggtccactac gctccagaca taggccgcca ggtatgagag gaagaagctg taggcccggc
      421 tgagcgtgat ctcgaccgtc gattcgtcga ccgccttgaa tccaattgca tccgaggtct
      481 tctgcagata cttgcgatag ttgcggaccg gctccatgaa ataggccatc ggcgagggct
      541 gatccggatt gagcgctcgc ttccacgagg tcacgaagtg atcggctgtg accttgttgc
      601 cattcgcgaa ttttgcatcc gagcgaatct tgaaggtgta ggttttgcca tcctcgctca
      661 gggtgtaact ctcggcaaga tccagctgca cctttgcgtt ctcgtcatac cggacaagac
      721 cgccccagac gttcgggaac atcgcccaaa acggccccca gttcaccgag cgatgaggat
      781 ccagcgtcac aacctggccg tacggatcga gcggcaccgt gatcgtgacg tccttctcga
      841 tgtcctgttg gcgaagaacc gccggcgccc cggatggccg agccggactg gctgccgcga
      901 caccggtgcg cattgtcgcg accgcccccg ccgcgatcgc cgaccacttg agcatgtccc
      961 ggcgggttcc tgacggttgg gaacgtcgcc acgcgcgctc aagttcggcc agttcattcc
     1021 gatctttcac catgctccct atctccttaa cctgggttgt gacaccagga gatcatctac
     1081 ccgggggggt ggttcctggg cctggcacca gacatccatg catcagcgcc aaagcgccgc
     1141 gcgtgtcgcc tgcggtcggg atccgagacg gtctgaacgc taccatgtac gccaataccc
     1201 cgcaaatgac ccgattaccg tgcactgcgg aaaacggatc acttcattcg actgcttgaa
     1261 cgaatgcggg acatcattcg ttccgaatcg tcaccgggcc gatttccaga acaggggaac
     1321 cggcgcctcg acgcaacgtc gaggcgccgg cttcgccgtg attgccgaga ttgttatggg
     1381 acgaccaaga tcgcgctcgt gtgcgcgtac ccggattcga tatgggtcac cgcggcgaac
     1441 gactgcgccg ctgaggccgc aaccggagtc cccaggaagt cggagattgc atcctgcccg
     1501 ctgatggtag tgctggctcc agcgcttgta gccgagagct gcgcgacatc gacgaagtag
     1561 acggcctggt actccgcggg gaggtacgac atcgccgtct tgaagctatc ggcgtcggcc
     1621 agggaatcgg tcggactgtt cagcgcagtc tcagctccgt cattcagtcc gagtacgagc
     1681 cgatcaccga tcacgccgta gtcgatcgcc acggtcgatc cggacgactg gaactcgaca
     1741 tgattgaccg aaccgcccgg gagctgcttc gaggtgacgt tgatgcgtcc ctgccccgcc
     1801 gcctgaacca gcaacgagat cgtgccaagg gagtcgccaa taatgccgga atccttggcg
     1861 cctgacaaga gcaccgcgtt gaccccgctg atatccgtcg catccgtgcc ccagacgccg
     1921 aaaccatacg gcccggtcat ctgatccagc agcgcggtct tcaggttgat accgaacgtg
     1981 cgagccagcg cctcgtagat cccatcgatt gacatcggca tcggggtggc ctggtcggtc
     2041 ggggtagcgc cgctcgagat tcccatgaag gcaagcccga taccgagccc aagcgcattc
     2101 atgagcatgc tgcgaccgag atcgaacccg cttgcgtaga ccaccgtatc ggctggcatg
     2161 cgggatgcga agtcgaggtc gacggcggag ccggccggct tggcgttggc ggtgccgctc
     2221 gccgggacga tgaccgattc gaaccggacc cccgcgtcat ccgccgtcat caccagcccg
     2281 gtgtatccgg agaaagtcgc gaacacttcg cggatcgcat cgcccatgga gcgatcgcca
     2341 gccgccttgg tcgcggcatc gatcgtgacc ggtccgttca ggaaggcata tgccacgcgg
     2401 tcggccggca atagatcgct ggtctttgtg aacgcatcca gattggcgag cgtgtccgct
     2461 cccggctggc tggcgtcgac gaacgccttg atatcgtcgg cggcggagcc ggcgaatagg
     2521 taatcaccaa cgaccgcata tgcggttgag ccgccttcgg tatcaccgta cgtggtgatg
     2581 tcggcgccct tgtacgtcac ggtcgcatta ttggcctcat cgctggccgt cttcttcatc
     2641 gaggcttcaa ccgacgcgat gtcggtcggc ttgatgatga caacagcgcc ctggttgcct
     2701 gcctggctgc tgacctgctg ggccagatcg ctggtggact cgccactcgt tgcgccaccg
     2761 acgatctcgc tcccgacgct tgctccttcg gcaagggcgc tcggcaggac cgcaaccgca
     2821 acttcagctc cggtcaggtc gatatcgctg ctcgggtcgg tagcggactt gttgatggca
     2881 tcgatcagcg actcggtgga tccgaggcgc gcgaggagct tgtcgaactg ctggagttgg
     2941 gcgctgctgg tgtccaggct gatctcagcg tagagaacga cgtcggcggg cgttacggac
     3001 gaggtcgaaa gcagaccttt ttcctgggca cgcgccggtg attggatcag aagccctgcg
     3061 ccgatggccg caagcaggga aagggccatc agcgcgcgca cggctcgctc caggccgccg
     3121 attcgaagtc gcgagtcgcc agacggaatg tgcatggtcc tgtctctcct actcaagcaa
     3181 gaaggccgac tccgtgtcag tcgacctccc aatccataaa cgcaatatgg ggagaccgcg
     3241 gttccctcgg gtagtggtcc tactccgaaa tgcggtcgtc gatgcctgta gcgagattga
     3301 agaagatcat gaagagcgcg gccaccaaac caatgattat gatgataatc gcgagtgtga
     3361 tccctgctcg caccaggccc atgattgtgt ccatcacgca ccctcccggc gctctaacga
     3421 cctgggtccg attatatccc ggatgcaaaa tgcggcagtc ctgaaacaac aggactgccg
     3481 cattcctgcg ccgactcgat cacgcgacca gcggcgcctc ggtgagctgc gcctggaatg
     3541 tcaggccggc gggcgacgca tcgatagtta ccacttcgcc gtcgtgcagc tcgccgttga
     3601 gcagtttcat ggccagcgaa tccagcacct cgcgctgaat cagtcgcttg agcgggcgag
     3661 caccgtagac cgggtcgtat ccccggctcc cgagccactc tcgcgcgcca ggcgtgatct
     3721 gcagctcgat attccgatcg gacagccgat ccgagactcg cttgagctga atctcgacga
     3781 tctctccgat ttgatcccgg cccagcggct cgaagatcac gagctcgtcg acacgattca
     3841 ggaactccgg ccggaagtgc tgccggaccg cctccatcac ccgctcgcgc atcgcctcct
     3901 ggtcgtgcgt gctcaacccc acgatcgagc tggacccgag gttgctggtc atgatgataa
     3961 cggtgttctt gaagtcgacg acccggccct ggctatcggt caggcgcccg tcatcgagca
     4021 cttgcagaag cgtgttgaag acatccgcgt gggccttctc gatctcatcg aagaggacca
     4081 cggagtacgg ccgtcgccgc acggcctcgg tgagctggcc gccctcctcg tacccgacgt
     4141 agcccggcgg cgcaccaatc agacgggcaa ccgcgtgccg ctccatgtac tcggacatgt
     4201 cgatccggac aattgcctca tccgtgtcga acaggaactc ggccagcgcg cgcgccagct
     4261 cagtcttgcc gactccggtc ggaccgagga agatgaagct gccgagcggc cggttcggat
     4321 cctgcaatcc cgcccgcgcc cgccggatgg cattggcgac agcggagata gcttcctctt
     4381 gcccgatcac gcgctcgtgg agccgctcct ccatgtgcag gagcttctcg acctcgcctt
     4441 ccatcagccg gctaaccggg attccggtcc agcgagcgac gatctcagca atgtcgtcgg
     4501 catcgacctc ttccttgagc agcttgccag aggcctgtaa ctttgtcagt gcctcttcct
     4561 gctcgcgaat ctgatcctga atctggacga gcttgccgta ctggagctcg gccgcggtgt
     4621 tgtagtcata ggatcgctgc gcctgctcga tctgggtgcg cgtctgatcg agctgattcc
     4681 ggagctcgga gatccgctgg atcgcgtcgc gctcctggtc ccagcgcgag cgcagagcgt
     4741 cgcgttgctc cttgagcgac gccaactcgc cctcgagcac ctcaagccga gcacgggatg
     4801 cgtcgtcccg ctccttgcgc agtgcctcgc gctcgatctc aagctgcatg atgcgccgtt
     4861 cggcctcgtc gagctcgacc ggcatcgacg tgatctccat ccgcagcttc gccgccgcct
     4921 catcaaccag gtcgatcgcc ttgtcgggca ggaatcgatc agcgatatac cggtgcgaca
     4981 gaaccgccgc cgcgacgagc gccgagtcga gaatccgcac ctggtgatgg acttcgtacc
     5041 gctcgcgcag accacgcagg atcgagatcg tgtcctcaac cgttggctcg ccgacgtaca
     5101 ccgtctggaa ccggcgctcg agggccgcgt ccttctcgat gtgcttgcgg tactcgtcaa
     5161 gcgtggtcgc gccgatcgca tgcaactcgc cacgcgccaa tgccggtttg agcaggttgg
     5221 cggcatccat cgcgccatcg gtccgaccgg cgccgacgac tgtgtgcaac tcgtcgatga
     5281 acaggatgat ctcgccgtcg gactcctgga cctccttgag gactgccttc agccgctctt
     5341 cgaattcacc ccggaacttg gcgccggcga tgagcgcgcc catgtcgagg gcgatgatcc
     5401 gcttgtcctg caatccctca ggcacgtcac cgcggacgat ccgctgggcc agcccctcgg
     5461 cgatggccgt cttgccgacg cccggctcgc caatcaggac cgggttgttc ttggtccggc
     5521 gggagagcac ctggatgacc cggcgaatct cctcgtcgcg accgatcacc gggtcgagct
     5581 ttccgtcctt ggccgccgca gtcaggtcgc gaccgtactt ttcaagcgac gcgtacgtgc
     5641 cctccggatt cggtgtagtc acgcgctgcg caccacgaat gcccttgagc agctccagga
     5701 cgcgatcgcg ggtcaggcca cgctcggcca gaagccgcgc tgcctggctc tgcgtcgcca
     5761 aaataccgag cagcaagtgc tcggtgctga tgtattcgtc gccaaactgg ctcgcttcct
     5821 gttgggcagt gtcgagcacc ttgcgggtcg cgctggagac ggttggttcc gagctgtact
     5881 ggagcgacgg caaggtgctg agcgcgcgct cgttcgcggc gatcaagtca ggaagatcga
     5941 tctgcgcctt cagcacgatc tgggggacga taccgtccga ctgggaaagc agagctgcga
     6001 ggattgcttc aggctcgagt tgcgagagct tccgttcctg ggtattccgc tgcgcctcga
     6061 cgattgcttc ctgcgcccgc tccgtaaacc gctgatttgc tgccatattt cgtttctacc
     6121 tccgctattg ctctgtcata cccttactgc tcgtcaattc agatccgact cacccgcggc
     6181 tggactccga cacaacgcct tcgcgccggc gaatctcggt catctgctcc tcgtgcacga
     6241 agcccaatgc gcgcaacatt cggtcgagct cagccgtcaa tgcggtgtat ccctcggcgt
     6301 cgccgtgatc ccggccgcga tcggacgtaa atgcgcgaat atccctcagc cggcgggtaa
     6361 tctcgagcac catctggaca ccggccaaat tgaggccgcg atcttcaacc aggtacttga
     6421 cctgtcggag ccgctcgatg tcctggtccg agtacaggcg aagattgccc tgcgtgcgcg
     6481 atggcgcgat caacccctcg cgctcgtatt tacgcaaggt ttgcggatgc atcttcacaa
     6541 ggcgcgcggc gacactgatg acgaacaga
//
LOCUS       DATAOZ010000062         6540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7656263, whole genome shotgun sequence.
ACCESSION   DATAOZ010000062 DATAOZ010000000
VERSION     DATAOZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7656263"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VHR64_01785"
     CDS             complement(<1..884)
                     /locus_tag="VHR64_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX3301580.1"
                     /translation="MIDPTTRMCPACGKTVGDTDKFCAACGAALTTAAAQPTPAPPGG
                     DARTVQRHRLGGTAYMATLPDSTEAEDDATAEVHAILNMSTDPTRVSGTGSTRSRSRS
                     GKRRKRRRHWYRRPILVVPLVAMMLLITVGAGLAWRATSTVNSLQRISTPPPAVDVAA
                     DEEDMAEVGGGPATPVTVDTAPAQQALVDAGYKPKDNGGVVDRVRVMSDVAQGASVAA
                     GIGNASAEPMTILIMGVDARPGDAIDIGVRPDVLMIVRLDPSAGGCRLMSIPRDTRTE
                     LPGYGLSKVNHALAVGGIP"
     gene            complement(1153..2271)
                     /gene="nagA"
                     /locus_tag="VHR64_01790"
     CDS             complement(1153..2271)
                     /gene="nagA"
                     /locus_tag="VHR64_01790"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX3301581.1"
                     /translation="MTVAGGLLLGGEFVPGSITINDGHITEVLRGAETDRLVDLEAAI
                     VAPGFIDLQVNGGYGAEVGADPAAILTLAARLPEAGVTAFLPTLITSPADVYQLVFEA
                     VRAAGAPPGAEPLGLHLEGPYLSQRRAGAHKLELIEAANDHLFESLLTAGDVRLMTLA
                     PERPGASERIRRLQERGIVVSLGHTDATYEQFVTGIDAGATMATHLYNAMSPFGHRSP
                     GVIGASLVDDRIAVGLIADGVHSHPASVRLAVRAKGVERIALVSDMMPAAGMPPGEYT
                     FGGQTVTVDANTARLADGTLAGSIATMDAAIRNVVRWTDATPAEALRMASEIPARILG
                     LPDRGRIKPGAIADLVLLDHDLVVQATIARGQIAYQRS"
     gene            complement(2285..3205)
                     /locus_tag="VHR64_01795"
     CDS             complement(2285..3205)
                     /locus_tag="VHR64_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX3301582.1"
                     /translation="MPQVATASELFGGVDGGGSKTLAVIVDCDGSERGRATAGSSNFT
                     AIGAEQAAAAISQAVTEAVEIAGGHLPVRSLWAGISGIDRQGAREQLQSLIQPLSGNI
                     RLTNDAELIFSALEGGVGVVLIAGTGSIALGHGTSGATARAGGWGHLMGDEGSGYDIG
                     RRALQAAARAADGRGPATALLEAFMRHWDVERPLDMIAHAYRSTDKAAVAELSSIVFA
                     AANDGDPVARRILSNAAGELASAAIAAGDQLALPDGVLPLALTGGLLAGNAEFRRSVL
                     RRIRARRRVGQVSIVTDSALSAARSLVRGK"
     gene            complement(3193..4125)
                     /gene="murQ"
                     /locus_tag="VHR64_01800"
     CDS             complement(3193..4125)
                     /gene="murQ"
                     /locus_tag="VHR64_01800"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX3301583.1"
                     /translation="MSSGVHDGLSELSTEGRNPASANIDCLSPLEIVRIMNDEDARVA
                     TAIEAELPQIAAAIEQIAERMGTRGGRLIYLGAGTSGRLGVIDASECPPTFSTPPGLV
                     VGWIAGGPQALTDAVEELEDSADAGRADIERLGITAADVVVGITASGRTPYVLGAIGA
                     ANERGAMTIGLACNANSDLARLVKIMIAPLVGPEVIAGSTRLKAGTAQKMVLNMLSTG
                     TMIRLGKTFGNLMVDLRATNSKLRQRAIRIVQEATGLDPSQAADLLRRSGDEAKTAIV
                     SARAGILPDEARSRLTQSRGSVRAALGEAAPCPK"
     gene            complement(4147..5292)
                     /locus_tag="VHR64_01805"
     CDS             complement(4147..5292)
                     /locus_tag="VHR64_01805"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX3301584.1"
                     /translation="MQTGLEILLAEGQPSLRGRRVGLITNHSAIDPKLRSAIDLLHES
                     DVVDLVALFGPEHGVRGDAQAGVKVEAATDKHSGLPVYSLYGETQHPTPEMLGGLDAL
                     LFDIQDIGVRYATYISTMANAMEAASDAGLTFVVLDRPNPLNGDAIEGNLLEPAFASF
                     VGVHPIPIRHGMTAGELARLIAAERGWAEPVVVEMTGWRRDHWFDDTELPWVQPSPNL
                     PTLDSVTLYSGTCMIEGTNVSEGRGTTRPFEYIGAPWLDPFDLADAMDKRNLPGCGFR
                     PAYFTPTFSKHYGAVCGGVQIHILDREKTRPAVLGLHLLDVIRSLDPQAFAWRQSSDG
                     GYFIELLLGSDQPRRMFDQGAGADEIVAQWADECASFAERRKPYLLY"
     gene            complement(5298..6020)
                     /locus_tag="VHR64_01810"
     CDS             complement(5298..6020)
                     /locus_tag="VHR64_01810"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3301585.1"
                     /translation="MSEQSNSDKCIMVIGAHAADAEIMGGASVLKHVDAGWRAVMVHM
                     TPGEKGHPKLSPAEYKAIKDVEAENAAKLLGAKSIMMPWPDGELPVNEDVQMAVADVI
                     REHKPTIILTHWKGSFHRDHNNTHHNVLASLFFAGLRTFEREFPPHRPEKIYFAENWE
                     DMENFKPEVYLDTTDVFDRYIEAIHAYSLFRKEVVPFRYEQWYRGASQMRGAESGFDQ
                     AVALMPHKSYYGSWHTLGLLDE"
     gene            complement(6127..>6540)
                     /locus_tag="VHR64_01815"
     CDS             complement(6127..>6540)
                     /locus_tag="VHR64_01815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3301586.1"
                     /translation="LHDEGIEIRPATPADIVPVLDFIPRHFTWDWHREASGVFNDLFA
                     GDPRFVGMMVAMQDGEVLGYAEHRGERFGPFGVHTGLRSRGIGRVLLAHTLHEMLKKN
                     FHAAWFLWTGDDAARLYTQVGFHEVRRFAVLKKRL"
BASE COUNT         1069 a         2084 c         2191 g         1196 t
ORIGIN      
        1 gggatgccgc cgaccgcgag ggcatggttc accttcgaga gaccgtatcc gggcagctcg
       61 gtgcgggtat cgcgcgggat cgacataagc cggcagccgc ccgcgctcgg gtcaagccga
      121 acgatcatca ggacatccgg ccgaacgccg atatcgatcg cgtcgccggg ccgggcatcg
      181 acgcccatga tcaggattgt catcggctcg gcactggcat tcccgatgcc ggcggcgacg
      241 gacgcgccct gggcgacgtc gctcatgacg cgcacacggt cgacgactcc accgttgtcc
      301 tttggcttgt agccggcatc caccagggct tgctgggcag gtgcggtgtc aacggtcacc
      361 ggcgtggcgg gtccacctcc gacctcggcc atgtcctctt cgtcggcggc aacatcgacg
      421 gccggtgggg gagtgctgat ccgctgcagc gagttgacgg tgctggttgc gcgccacgcc
      481 agtcccgcgc cgaccgtgat cagaagcatc atggcgacga gcggaacgac gaggatgggt
      541 cggcggtacc agtgccgccg tcgcttgcga cgtttcccgg agcgcgatcg ggatctggtg
      601 gaaccggttc cgctgaccct ggtcgggtcg gtggacatgt tgaggatcgc gtgtacctcg
      661 gcggtcgcat cgtcttccgc ctcggtcgaa tcgggcagcg tcgccatgta cgcggtgccg
      721 ccgaggcggt ggcgctggac ggtgcgtgcg tcgccacctg gaggagctgg ggtgggttgg
      781 gcggcggctg tcgtgagcgc tgctccgcag gcagcgcaaa acttgtcggt gtcaccgacg
      841 gtctttccac aggcgggaca catgcgtgtc gtcggatcga tcaacctgct tgctgtctcc
      901 cggtcatggc tctgggtatt ccgctcctgc gtcggtcaag cgacatcatc agcatcgatt
      961 tggcccgact ggacacgccg cgtgggctga ggatctcgcg acattcaggc ggcgctacgc
     1021 ttcgaaccga ctccacaacc acaatcagat cgtactgcga aacaactact tctgcggggc
     1081 ggatagttgg aaataatgtc acgacaggaa agaatactcc acctgtcctt cggcagtgac
     1141 gagagcgctg actcaggaac gttggtacgc gatctggccg cgagcaattg tcgcctgaac
     1201 gaccaggtcg tgatcgagca ggacgaggtc agcgattgcc cctggcttga tacgtccacg
     1261 atccgggagg ccgaggatgc gggcagggat ctcactcgcc atgcggagcg cctcggccgg
     1321 tgttgcatcg gtccagcgga cgacattgcg gattgccgcg tccatcgtcg cgatcgagcc
     1381 ggccaatgtg ccgtcggcca acctggcggt gtttgcgtcg acggtgacag tctgcccacc
     1441 gaacgtgtac tcgcctggtg gcatcccggc ggccggcatc atgtcggaga cgagcgcgat
     1501 ccgttcgacc cctttggcgc ggacggccag gcggacgctc gccgggtgac tatggacccc
     1561 gtcggcaatg agtccaacgg cgatccgatc atcgaccagt gacgcgccga tcaccccggg
     1621 cgaccggtgc ccgaacggcg acatcgcgtt gtagagatga gtggccatgg ttgcaccggc
     1681 gtcgattccc gtgacgaatt gctcgtaggt cgcgtcggtg tggccaagac tgacgacaat
     1741 cccgcgctcc tgaagccggc ggattcgctc gctcgcgccc ggacgttccg gggcgagtgt
     1801 catgagccgc acatcgccgg cggtcagcag cgattcaaac aggtggtcat ttgcggcttc
     1861 gattagctcg agcttgtgcg ctccggcccg gcgttgggaa aggtacggtc cctcaagatg
     1921 gaggccgagc ggttcggcgc cgggcggggc acctgccgcg cggacagcct cgaagacgag
     1981 ctgatagacg tcggctggcg acgtgatgag cgtcggtaga aacgcggtca ctccggcttc
     2041 cgggagtcgt gccgcgaggg tgaggattgc ggctgggtcg gcgccgactt ccgccccata
     2101 cccgccattg acctgcagat cgatgaaccc gggcgcgacg atagcggctt caaggtccac
     2161 gagtcgatct gtttcagccc cccgcagcac ctcggtgatg tggccatcgt taatagtgat
     2221 tgatccagga acgaactcgc cgccgaggag gaggccgcct gcaacagtca tcgaggacat
     2281 agcgtcactt gcctctgacc agactgcggg ctgcactgag agcggaatcg gtgacgatgc
     2341 tgacctggcc gacccggcgg cgcgctctga tgcggcgcag caccgatcgc cggaattcgg
     2401 cgtttccggc cagcagtccg ccggtgagcg ccaatggcag gacgccgtcc ggcaatgcga
     2461 gctgatcgcc ggccgcaatc gccgcgctcg ccagttcgcc ggccgcattc gacaggattc
     2521 gcctggcaac ggggtcgccg tcatttgcgg cggcaaagac gatcgacgac agctctgcga
     2581 cggcagcctt gtcggtcgag cggtaggcgt gggcgatcat gtcgagcggt cgttcgacgt
     2641 cccagtgacg catgaatgcc tcgagcagcg ccgtcgctgg accacggccg tccgcggcac
     2701 gggccgctgc ctggagcgcg cgccggccga tgtcatagcc gctgccctcg tcgcccatca
     2761 ggtggcccca gccgccggcg cgcgcagtcg ctcccgatgt gccgtgaccg agtgcgatcg
     2821 agccggtccc cgcgatcagg accactccga cgccgccctc gagcgcgctg aagatgagct
     2881 cggcgtcgtt ggtcaggcgg atattgcctg acagcggctg aatcagcgat tgcaattgct
     2941 cgcgggcacc ttggcggtcg atcccggaaa ttccggccca gagtgatcgg accggcaaat
     3001 gcccgccggc gatttccact gcctcagtga ctgcctgcga gattgcggcg gcggcctgct
     3061 cggctccgat ggccgtgaag ttcgagctgc ccgcggtggc gcgaccgcgc tcacttccgt
     3121 cgcagtcgac aataaccgcg agtgtcttcg agccgccgcc gtcgacgccg ccgaacagct
     3181 ctgacgccgt tgctacttgg ggcatggagc cgcctcgccg agtgctgccc tcaccgaacc
     3241 acgggattgc gtgagtcgcg atcttgcttc gtcgggcagg atgccagccc gggccgagac
     3301 gatggctgtc ttggcttcgt cgccggatcg tcgcagcagg tcggctgcct gcgatggatc
     3361 gaggccggtg gcctcctgga cgatgcggat ggcgcgctgg cggagcttgg agttggtggc
     3421 ccggagatcg accatgaggt tgccgaaggt cttgccgagg cggatcatcg tgccggtgct
     3481 gagcatgttt agcaccatct tctgggccgt tcccgccttg aggcgggtcg aaccggcgat
     3541 gacctccggg ccgaccagcg gagcgatcat gatcttgacc agtcgcgcga gatccgagtt
     3601 cgcgttgcag gccagaccga tggtcattgc tccgcgttcg ttggcggccc cgattgcgcc
     3661 aaggacgtac ggcgtgcggc cgctagccgt gatgccgacg acgacgtcgg cagccgtaat
     3721 tcccaggcgc tcgatatcag cgcggcccgc gtcggcgctg tcttccagct cctcgacggc
     3781 gtcggtcagg gcctggggac cgccggcgat ccagccgacg accaggccgg gcggcgtgct
     3841 gaaggtcggc gggcattccg aggcgtcgat cacgccaagt cggcccgatg ttccggctcc
     3901 gagatagatc aggcggccgc cgcgcgtgcc catgcgttcg gcgatctgtt cgatggcggc
     3961 ggcaatctgc ggaagttcgg cttcgattgc cgtcgcgacc cgggcgtcct cgtcgttcat
     4021 gatccgaacg atctcgagcg ggctcaggca gtcgatattc gcgctcgccg ggttgcgccc
     4081 ctcggtggat agctcactta gaccgtcatg taccccggat gacatatcca accctcatta
     4141 ccatcatcaa tacagaaggt atggcttcct ccgctcggcg aagctcgcac actcgtcggc
     4201 ccactgcgcc acgatctcgt ccgcgcccgc tccctggtcg aacatgcggc gtggctggtc
     4261 gctgccgagg agcagttcga tgaagtagcc gccatcgctc gattgccgcc atgcgaacgc
     4321 ctgcggatcg agcgaacgga tcacgtccag caggtggagg ccgagcacag ccggccgcgt
     4381 tttctcacgg tccaggatgt ggatctggac gccgccgcag actgcaccgt agtgcttcga
     4441 gaaagtcggc gtgaagtagg ccggccggaa gccgcatcct ggcagattgc gcttgtccat
     4501 cgcgtcggcg agatcgaacg gatcgagcca cggcgcgccg atgtattcga acggccgggt
     4561 ggtcccgcgg ccttctgaaa cgttcgtgcc ctcgatcatg caggtcccgg aataaagggt
     4621 gaccgagtcg agcgtgggta ggttcggcga cggctgcacc catggcagtt cggtgtcgtc
     4681 gaaccagtga tcgcgccgcc agcccgtcat ctcgacgacg accggctcag cccagccgcg
     4741 ctccgcagcg atcaggcgag cgagctcgcc tgccgtcatc ccgtgccgaa tggggatcgg
     4801 gtgcacgccg acgaacgagg caaaggcggg ttcgagcagg ttgccctcga ttgcgtcgcc
     4861 gttgagcggg ttcggccgat cgagaacgac gaaggtcagg ccggcatccg aggcggcttc
     4921 catcgcgttc gccatcgtcg agatgtatgt ggcgtagcgg acgccgatgt cctggatatc
     4981 gaaaagcagc gcgtcgagac cgcccagcat ctcgggggtc gggtgctgtg tctcgccgta
     5041 gaggctatag accggcaggc cggagtgctt gtctgtggct gcctcgacct tgacgccagc
     5101 ctgggcgtcg ccgcggacgc cgtgctccgg gccgaacagc gccacgaggt cgacaacgtc
     5161 gctctcatgg agcaggtcga tcgcgctccg caacttcgga tcgatcgccg aatggttcgt
     5221 gatcaatccg acccgccggc cgcggaggga cggttgtccc tccgccagca gaatttcgag
     5281 tcccgtttgc accgtgctta ctcgtcgagc aggccgagcg tatgccagga gccgtagtag
     5341 ctcttgtggg gcatcagcgc gaccgcctgg tcgaatccgg actcggcgcc gcgcatctgg
     5401 ctcgcgccgc ggtaccactg ctcgtaacgg aacggcacga cctccttgcg gaagaggctg
     5461 taggcgtgga tcgcctcgat gtagcgatcg aaaacatcgg tcgtgtcgag gtagacctcc
     5521 ggcttgaagt tctccatgtc ctcccagttc tcggcgaagt agatcttctc cggccggtgg
     5581 ggcgggaatt cgcgctcgaa cgtgcggagt ccggcgaaga agagcgacgc caagacgttg
     5641 tgatgggtgt tgttgtgatc acggtggaag ctgcccttcc agtgggtcag gatgatggtc
     5701 ggcttgtgct cgcgaatcac gtccgcgacc gccatctgga catcttcgtt gaccgggagc
     5761 tcgccatccg gccagggcat catgatcgac ttggcgccga gcagtttggc ggcgttctcg
     5821 gcctcgacgt ccttgatcgc cttgtactcg gccggcgaga gcttcgggtg tcccttctcg
     5881 ccgggtgtca tgtggaccat gaccgcgcgc caaccggcat cgacgtgctt gagcaccgac
     5941 gctccaccca tgatctcggc gtcggcggcg tgcgcgccga tcaccatgat gcacttgtcg
     6001 ctgtttgact gctccgacat caacttctcc ctttttggac ttcagacttc aaacttcgga
     6061 cttcaggttt cagacctcag attcctagtt ctgacgtccg aagtccgagg tctgacgtct
     6121 gatgtctcat aaccgctttt tcaggacggc aaagcgccgt acctcatgga acccgacttg
     6181 tgtgtagagc cgcgccgcgt cgtctccggt ccagaggaac caggcggcgt ggaaattctt
     6241 cttcagcatc tcgtgaaggg tatgggccag caagacccgg ccgatgcccc ggcttcgaag
     6301 tccggtatgg acgccgaatg ggccgaaccg ctcaccgcga tgctccgcgt accccagcac
     6361 ctccccgtct tgcatggcga ccatcatgcc cacaaagcgc gggtcgccgg cgaacagatc
     6421 gttgaacacg ccgctggcct cgcggtgcca gtcccacgtg aagtggcggg ggatgaagtc
     6481 gagcaccggc acgatgtcgg ccggcgtggc gggtcggatc tcgatgccct catcgtgcag
//
LOCUS       DATAOZ010000063         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8446115, whole genome shotgun sequence.
ACCESSION   DATAOZ010000063 DATAOZ010000000
VERSION     DATAOZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8446115"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VHR64_01820"
     CDS             <1..636
                     /locus_tag="VHR64_01820"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEX3301587.1"
                     /translation="GRQDGPHAHMIRATPDGRYVLATDLGADRIFVFRLNLESGQLEP
                     NDAGAASAASAPGTGPRHFAFAPDGRTLYVINELASSLTAWAWDAGSGSLNQIETVSM
                     LPEGFDGTNSGAEVVVSPDDRFVYASNRGHDSIAIFAVGEGGLTLVGHESTQGKSPRG
                     FALDPTGAWLLAANQRSDSIVVFRRDPATGRLNATDQVVHSPTPVAIVFTE"
     gene            736..1239
                     /locus_tag="VHR64_01825"
     CDS             736..1239
                     /locus_tag="VHR64_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301588.1"
                     /translation="MSADERTITGHNIEYQRREWVIQRIGWMAMLLAIVAAFLGLLGN
                     GPLTDATASSSDGAVSVEYFRIARHHQPRTLSVTVAPDVVENGEVKIWIDRAYADQFG
                     LENIVPDPDSADVEPDRVIYTFTTGQANGPLTVTFSYNHDGFWRQHGRVGLIGGPTIS
                     FTQFLFP"
     gene            1272..1724
                     /locus_tag="VHR64_01830"
     CDS             1272..1724
                     /locus_tag="VHR64_01830"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX3301589.1"
                     /translation="MDEVLRAIAIYAVLMVIFRLSGKRSMAEVTPFDFVLLLIFSEAL
                     QNALVDDDSSLTVGLTVVATLLMVNLIMSLIKQRSVRIENILEGVPIVIFDEGKPLHD
                     RMNRERIDEGDILAAARMQHGIDSLDQVRTAIIERSGGISIIPKQSEQ"
BASE COUNT          340 a          571 c          563 g          344 t
ORIGIN      
        1 gggcggcaag atgggccgca tgcgcacatg atccgggcga cgccggacgg gcgatacgtg
       61 ctcgcgaccg atctcggcgc ggaccgaatc ttcgtcttcc ggctcaacct cgaatcaggc
      121 caactcgagc ctaacgacgc cggagccgcc tcggctgcat ccgcaccggg aaccggaccg
      181 cgccacttcg ccttcgcgcc ggatggtcgc acgctctacg tgatcaacga gctggcctcg
      241 tcgctgacgg catgggcgtg ggacgccggg tcgggatcgc tcaatcagat cgagaccgtc
      301 tcgatgctgc ccgagggatt cgacggtacc aattcgggcg cggaggtcgt cgtctcgccg
      361 gacgaccgct ttgtctatgc ctcgaatcgc ggtcacgata gcatcgcgat cttcgcggtg
      421 ggtgaaggcg gattgacgct ggtcgggcac gaatcgacgc aaggcaagag tccacgcggc
      481 tttgccctcg atccaaccgg cgcctggctg ctggccgcca accagcgctc cgactcgatc
      541 gtggtcttcc gtcgcgaccc ggcaaccggc cggctcaatg ccaccgatca ggtcgtccac
      601 agcccaactc cggtcgcgat cgtcttcacc gagtaatcgc cagttcggca ccgtagccgc
      661 tctgcatagc tcctccgcct tgcccggcaa tactcccgag acggtacaga tccgcgattc
      721 gggaaggagg caggcgtgtc tgcggatgag cgcacaatta ccgggcacaa catcgaatac
      781 cagcggcggg agtgggtcat ccagcgcatt ggctggatgg cgatgctgct ggcgattgtg
      841 gcggcattcc tcgggctgct tggcaacggc ccgctgaccg atgctactgc gtcctcaagc
      901 gatggcgccg ttagcgtcga atacttccgc atcgctcgtc accaccagcc gcgaaccctg
      961 tcggtcacgg tcgcaccgga cgtcgtcgag aatggcgaag tcaagatctg gattgaccgg
     1021 gcctacgccg atcagttcgg actcgagaac atcgtgcccg atccggacag cgccgacgtc
     1081 gagccggatc gcgtgatcta caccttcacg accggccagg cgaacggtcc tcttacggtc
     1141 accttctcct acaaccacga cgggttttgg cggcagcatg gccgggtcgg cctcatcggc
     1201 gggccaacga tcagcttcac acagttcttg tttccctagg ccaacgggcg cagagatcca
     1261 ggagaagaga gatggacgaa gtccttcgcg cgatcgcgat ttacgccgtt ctcatggtga
     1321 tcttccgcct ctcgggcaaa cgctcgatgg cggaggtgac accgttcgat ttcgtgctgc
     1381 tgctgatctt cagcgaggcg ttacagaatg cgctggtcga cgatgacagc tcgctgacag
     1441 tcggcctgac cgtggtcgcg accctcttga tggtgaacct gatcatgtcg ctgatcaagc
     1501 agcgctcggt ccggatcgag aacatcctgg aaggtgtgcc gatcgtgata tttgacgaag
     1561 gcaagccgtt gcacgatcga atgaatcggg aacggatcga cgaaggggac atcctggcgg
     1621 cggcgcggat gcagcatggc atcgacagcc ttgatcaggt ccgcaccgcc atcatcgaac
     1681 gctcgggcgg gatctcgatc atcccgaagc aatcagagca gtaatcgaac ctacccacat
     1741 ggcaacgagc ggagaacgtg attctccgct cgtctcgttg ctgtttctga atcgtttgtg
     1801 gcggctgtcg gtgatttg
//
LOCUS       DATAOZ010000064         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2133163, whole genome shotgun sequence.
ACCESSION   DATAOZ010000064 DATAOZ010000000
VERSION     DATAOZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133163"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VHR64_01835"
     CDS             <1..582
                     /locus_tag="VHR64_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3301590.1"
                     /translation="PGLERRAVTGKYSLRAAAATEFEMHDVRLPADAVLPGVTGLAGP
                     LACLSEARYGIIWGMLGVMRSCLETALEHSTTRVQFGKPIGAYQLTQAKLTDMLLTYS
                     QAQLLAHQIGRLKQRGELTTEQVSMGKLNNVNAAIEVARTARGILGGNGITSMYPVMR
                     HMANLESILTYEGTAEVHTLIIGQGLTGLSAFR"
     gene            complement(800..1132)
                     /locus_tag="VHR64_01840"
     CDS             complement(800..1132)
                     /locus_tag="VHR64_01840"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX3301591.1"
                     /translation="MPFAQREAPMPSMTIVVPHQLTQEEAMRRIQNLLSDVKRDYGDR
                     VSDLQETWSGNSGEFSFKAMGFSLSGRLDVKPGEVVMKGNYPLAAVPFKGKIEGMIRE
                     RAEQLLAS"
     gene            1288..2670
                     /locus_tag="VHR64_01845"
     CDS             1288..2670
                     /locus_tag="VHR64_01845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3301592.1"
                     /translation="MAVRTSPRSSLSPDSVDNLRRQITGNVLLPNEDGYDTARTVWNA
                     MIDRSPTLIVQPESTADVVAAVRFAAEQDLPIAVRGGGHNVAGLAVCDDGLMIDLSRM
                     RQVDVDPERKIARAQGGATWADFDAATTAHGLATTGGAISTTGIGGLTLGGGLGNLMR
                     SYGLASDNVLSCEVVTASGDVVTASTAENPDLFWGLRGGGGNFGVVTMLEYRLHPVSQ
                     VLGGLLIFPVDRARDLIRLYRQVTASAPDALGVSFVLTHAPDGTPVCALAICYNGPAD
                     EGERVIKPFRDFGAPIMGEVGPVPYTATQTMVDEAFPPGLQNYWRSHFLTDLTDEGMA
                     ILIDHFSRVSSPLAGLLIEDLGGAVARVNPDATAFNFRNYTYNLAILGRWAEPSGADA
                     GIAWTRGVHEAMQPFAAGVYVNYLSVGEQSDRVRQAYGEEKYARLVELKNRYDPSNRF
                     CFNQNIPPDA"
BASE COUNT          502 a         1021 c          897 g          499 t
ORIGIN      
        1 cccggcctcg agcgccgcgc ggtcaccggc aagtactcgc tccgcgcggc cgccgcgacc
       61 gagttcgaga tgcacgacgt ccgacttccg gccgatgccg ttctccctgg tgtgaccggc
      121 ctcgccggac cgctcgcctg cctgagcgaa gctcgctacg ggatcatctg gggcatgctc
      181 ggtgtcatgc ggagctgcct cgagacggcg ctcgagcaca gcacgacgcg cgtccagttc
      241 ggcaagccga tcggcgccta tcaactcacg caggccaagc tgaccgacat gctgctcacg
      301 tacagccagg cccagctcct cgcccaccag atcggccgcc tcaagcagcg cggcgagctg
      361 accaccgagc aggtcagcat gggcaagctc aacaacgtca acgccgcgat cgaggtcgcg
      421 cgcacggccc gcggcatcct cggcggcaac ggcatcacca gcatgtaccc ggtcatgcgc
      481 cacatggcca acctcgaatc gatcctcacc tacgaaggca ccgccgaagt ccacaccctc
      541 atcatcggcc agggcctgac aggcctgtcc gcgttccggt gagttgaggg tggagggttg
      601 agggaacgaa aaaggggtga cgcaaatctc tgtagggatt tgccgtggca aatctgtccg
      661 cggccaaatc gagcgcggcg tgatgtggaa tatgggattg gcgccggttg cgagattggc
      721 gctgggcgcc agacccgcgg atccaggcgc gggtttgcca cggcaaatcc cctacacagg
      781 ggcgtgcgtt ttcggttgct cagcttgcca gcaattgctc cgctcgttcg cggatcatcc
      841 cttcgatctt gcctttgaac ggcactgcgg ccagcgggta gttccccttc atcacgacct
      901 cgcctggctt gacatccagc cggccggaaa gcgagaagcc catcgccttg aatgagaact
      961 cgccggagtt gccggaccac gtctcctgca ggtcgctgac ccgatctccg taatcccgct
     1021 tcacgtcact cagcaggttc tggattcggc gcatcgcctc ctcctgcgtc aactgatgtg
     1081 gaacaacgat cgtcatgctc ggcattggcg cctccctctg tgcgaacggc atctcgctct
     1141 ccagtctgcc agattcggcc ttgttaccgg ccgatcccgc gcacgttcaa gcgatcgcga
     1201 cgcttgacat cgcatgcgct caccctagaa tcggtaacgc actgcacaga tcgactcatg
     1261 tctccgcatc tcgcgaaagg cagcaccatg gccgtcagga ccagtccgcg ttcctcactg
     1321 tcccccgact cggtcgacaa cctccgccgg cagatcacgg ggaatgtcct gctcccgaac
     1381 gaggacgggt atgacaccgc ccgtaccgtc tggaacgcca tgatcgaccg ctcgccgacc
     1441 ctgatcgtcc agccggagag cacggccgac gtcgtcgcgg cagttcgctt cgctgccgag
     1501 caggatttgc caatcgcggt ccggggcggc gggcacaacg tggccgggct ggcagtctgc
     1561 gacgacggcc tgatgatcga cctctcccgg atgcgccagg tggacgtcga ccccgagcgg
     1621 aagattgccc gggcccaggg cggcgccacc tgggccgact tcgacgccgc aaccacggct
     1681 cacggcctgg cgacgaccgg cggggcgatc tcgaccaccg ggatcggcgg tctcaccctc
     1741 ggcggcgggc tcggcaacct gatgcgctca tacggcctgg ccagcgacaa cgtgctctcc
     1801 tgcgaggtcg tcacggcgag cggggatgtc gtcaccgcaa gcacggccga gaatccggac
     1861 cttttctggg gcctgcgcgg tggcggcggg aacttcggcg tcgtcaccat gctggaatat
     1921 cgcctccacc cggtcagcca ggtgctcggc ggtctgctga tattcccggt cgaccgcgcc
     1981 cgcgacctga tccggctcta ccggcaagta accgcgagcg caccggacgc tctcggcgtg
     2041 tcattcgtcc tgacgcacgc tccggacggc acgccggtct gcgccctggc catctgctac
     2101 aacggcccgg ccgatgaggg cgagcgcgtg atcaagccgt tccgcgattt cggggcaccg
     2161 atcatgggcg aggtcgggcc ggtgccctac acagcgaccc agaccatggt cgacgaggcg
     2221 ttcccgccgg gcctgcagaa ctactggcgc tcgcactttc tgacggacct gacggacgag
     2281 ggcatggcga tcctgatcga tcatttcagc agggtctcct caccgctcgc cggtctcctg
     2341 atcgaggatt tgggtggagc cgtcgctcgg gtcaatccgg acgcgaccgc cttcaacttc
     2401 cgaaactaca cctacaacct ggcgatcctc ggccgctggg cggagccatc cggggctgat
     2461 gccggcatcg cctggacgcg cggcgttcat gaggcaatgc agccgttcgc cgctggtgtc
     2521 tatgtcaact acctcagcgt cggcgagcaa tcggaccgcg tccgccaagc ctatggtgag
     2581 gagaagtacg cccgcctggt cgagctcaag aaccgctacg acccgtccaa tcgcttctgc
     2641 ttcaaccaga acatcccgcc agacgcatag acgatacgca gacgggagcc ggcgcattgc
     2701 cggctcccgt ttgccatccc tacagccgcg tgcatttaac ttgaccgttc ccggtacgta
     2761 gcgcggggtt accacgcccc gctcgtcgat ccctgtgccg ggagatagag tcgagccgtg
     2821 cccggggtcg gttcccccaa cggcatggcc gacatcgaat ccgggcggcg cgtgggaacg
     2881 cgctacatca ggcccttcat aactccgcgc tagtcccgg
//
LOCUS       DATAOZ010000065         3114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2134156, whole genome shotgun sequence.
ACCESSION   DATAOZ010000065 DATAOZ010000000
VERSION     DATAOZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134156"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VHR64_01850"
     CDS             complement(<1..1234)
                     /locus_tag="VHR64_01850"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX3301593.1"
                     /translation="MLLLGASGDLSGRLLLPALGQLLDREEQRRDVVLIGAGSEPWEA
                     DAWRDRLKTSFESGDVSDETLKSVLDTTHYKQVDVTSVEDLKALIDSCPPAPAIYFAL
                     PPSVTGAACSALQQVSLPEGTTLVLEKPFGSDEESAASLNQLLAELVPERQVHRVDHF
                     LGRSTVLNLIGVRFANRLFEPLWSNEHIERVDIVFDEQLTLENRARYYDKAGALMDMI
                     QSHLLQVLALGAMDPIATINAVDLRDAKAEVLRACRPWNNDPVAAGRRARYIQGSIDG
                     RDVPDYVAEPGVDPARNTETLAELVVEVDNWRWAGVPFRLRSGKALRERRKEVLITFK
                     RLPHLPTGLRGEPIPERLRLAMGPDRMALEMNVNGPDDPLALDRVALDTDLNPGRLPA
                     YGEVLAGVLDGDPLLSVRG"
     gene            complement(1365..1922)
                     /locus_tag="VHR64_01855"
     CDS             complement(1365..1922)
                     /locus_tag="VHR64_01855"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX3301594.1"
                     /translation="MSITTEQLRAAYLRSGNVYAGSEKLGRIGQIYLDDETSEPTWVT
                     VKTGLFGTSESFVPLNSARLEGDDILVDHDKDTIKNAPRIDADGDLTPREELALFRYY
                     SRPRAADSTDWEDRTDRDDDRLNRDDDLRDRDDDLRDRDDLPDRDTAQDHQRVVDEVA
                     DRREENRSERGRLRRYVVTEITEDR"
     gene            complement(2060..2563)
                     /locus_tag="VHR64_01860"
     CDS             complement(2060..2563)
                     /locus_tag="VHR64_01860"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3301595.1"
                     /translation="MSTNAKPEAATRETTIEADPDLPTIRIVRDFDAPREKVFRAFID
                     PELVAQWLGPKSSEMRIDEWTARTGGNYRYASVANGEEVAAFYGSFHEVRPNERLVQT
                     FTWEGMPDGVSLETMTFEDLGDGRTRTIGLSLVDNLQARDAIMASGMDVGVNEGYEKL
                     DALLAEG"
     gene            complement(2560..2934)
                     /locus_tag="VHR64_01865"
     CDS             complement(2560..2934)
                     /locus_tag="VHR64_01865"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3301596.1"
                     /translation="MSGDRLSQVFAALADPTRRDIVARLSVGDATVSQLAAPYNVSIQ
                     AVSKHLKVLEDAGLVSRSHDAQRRPRHLEAEVFDMMTAWIERYRKQAEERYRRLDAVL
                     AAMDDNSEPKINQPTTNQGAAS"
BASE COUNT          548 a          988 c          944 g          634 t
ORIGIN      
        1 ctccacgcac cgacagcagc ggatcaccgt ccaacacacc cgccaagact tctccgtacg
       61 ccggtaaccg tcccgggttg aggtcggtgt cgagggcaac ccgatccagc gccaaagggt
      121 cgtctggacc attcacattc atctccagcg ccatccggtc cgggcccatg gcgaggcgca
      181 atcgttccgg aatcggctcg ccccggagcc ccgtcggcag atgtggcaac cgcttgaagg
      241 tgatcaagac ctctttacga cgttctcgga gtgccttgcc ggaccgtagc cggaagggca
      301 cgccggccca ccgccagttg tcgacctcga cgacgagctc tgctagcgtc tcggtgtttc
      361 gcgccggatc gaccccgggc tctgcgacgt agtcgggcac gtccctgccg tcgatcgagc
      421 cctggatgta tcgcgcccga cgaccggccg caaccgggtc attgttccaa ggccggcagg
      481 cccgcagcac ttcggccttc gcgtcgcgaa gatcaactgc attgatggtc gctatcggat
      541 ccattgcccc cagcgccaac acctggagca gatgactttg gatcatgtcc atcagcgcgc
      601 cggctttgtc gtagtagcga gctctgttct cgagggtgag ttgctcgtcg aacacaatgt
      661 cgacgcgctc gatgtgttcg ttgctccaga gcggctcgaa cagccggttg gcaaagcgca
      721 cgccgatcag attgagcaca gtggagcgtc cgaggaaatg atcaacgcga tgtacttgcc
      781 gttccggcac gagctcggca aggagctgat tgaggctagc cgcgctttcc tcgtcgctgc
      841 cgaagggctt ttccagtacc agggtcgtgc cctccggcag tgacacctgc tgcagggcgc
      901 tgcacgcggc accggttacc gaaggtggca gtgcaaaata gatggccggt gccggcgggc
      961 aagaatcgat cagcgccttc aagtcttcga cgctggtcac atcaacctgc ttgtaatggg
     1021 tcgtgtccag cacgctcttc agcgtctcgt cggaaacatc gccggactcg aaggaggtct
     1081 tgagccggtc gcgccatgcg tcggcttccc agggttcgga gcccgcaccg attagcacga
     1141 catctcggcg ctgctcttcg cggtcgagca gctgtccgag agccggcaat agcaaccgtc
     1201 cggagaggtc gccgctggcg ccaagcagca gcaagcttcc gatggaacga gtcatcgcgc
     1261 ctcctcagtg caacccaaag cgtattcggg ctgattacct gaggcggtac ccgctcacat
     1321 ctttacgcaa accggcaggc gacgggcgta cggctgctgg ctcgttagcg atcttcggtg
     1381 atctcagtta cgacgtaccg gcgcagacga ccacgctccg agcggttctc ctcacgcctg
     1441 tcggcgacct cgtctactac tcgttggtga tcttgagccg tgtctcgatc aggcaggtcg
     1501 tcccgatcac gcaggtcatc gtctcgatca cgcaggtcat cgtctcgatt cagccgatca
     1561 tcatcgcgat cggttctgtc ctcccaatcg gtcgaatcgg cggcgcgcgg ccggctgtag
     1621 taacggaaaa gcgccagttc ctcgcgcggt gtcagatcac cgtcggcgtc gatccgcggg
     1681 gcgttcttga tcgtgtcctt gtcgtggtcg acaagaatgt cgtcgccttc gagccgcgct
     1741 gaattcagcg gcacgaatga ctctgaggtt ccaaataatc cagtcttgac cgtgacccag
     1801 gtcggttcag aggtctcatc atcgaggtag atctggccga tccggccgag cttctctgag
     1861 ccggcgtaca cattcccaga cctcaaatag gcagcccgaa gctgttctgt ggtgatgctc
     1921 atggcgcgtc cccttccctt tctctaggaa ggttccttaa ccaacggcat cgggcacgaa
     1981 acagacagcc tcaaacaaac ttgtcagcgt ctcagcgggt gatagcccgc tgagacgctg
     2041 acaagtcgga ggctgggttt cagccctcag cgagcaaggc gtccagtttt tcgtagccct
     2101 cattgactcc gacatccata ccgcttgcca tgatcgcgtc gcgggcttgg aggttatcga
     2161 ccaacgagag accgatcgtc cgggtccggc catcgccgag gtcctcgaac gtcatggtct
     2221 ccaggctgac gccatcgggc atgccttccc aggtgaaggt ctggaccagc cgctcgttcg
     2281 gtcgcacctc atggaaggac ccgtagaacg cggccacctc ttcaccgttt gcgaccgagg
     2341 catagcgata gttacctccg gtgcgcgcag tccactcgtc gatcctcatc tcgctgctct
     2401 tcgggccgag ccactgggcg accagttcgg gatcgataaa cgcccggaag accttctcgc
     2461 gcggagcgtc gaagtcgcga acgatgcgga tggtcggcag gtcgggatcg gcctcgatgg
     2521 tggtctcgcg cgtcgcggct tccggcttgg catttgtgct catgatgctg ctccttggtt
     2581 ggtagttggt tgattgatct ttggttcgct gttgtcgtcc atggcggcga gtacggcatc
     2641 gagccgccga tatcgctcct cggcttgctt gcggtagcgc tcgatccaag cggtcatcat
     2701 gtcgaatacc tccgcttcaa ggtgtcgggg tcggcgctga gcatcgtggc tgcggctgac
     2761 cagtccggcg tcctccagta ccttcaggtg tttggacacc gcctgaatcg acacgttgta
     2821 cggcgccgcc agttggctca ccgtggcgtc cccgacggag agccgagcga cgatgtcgcg
     2881 ccgcgtgggg tcggccaagg ctgcgaagac ttgggatagt cgatccccgc tcatgcctgc
     2941 ctccttggcc tgcttattca accacttggt tgaatacggc gagggaggcg gaaatgaacg
     3001 aggatcgact atcccaagtc ttcgcagcct tggccgaccc cacgcggcgg gacatcgtcg
     3061 ctcggctcgc cgtcggggac gccacggtga gccaactggc ggcgccgtac gacg
//
LOCUS       DATAOZ010000066         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6079031, whole genome shotgun sequence.
ACCESSION   DATAOZ010000066 DATAOZ010000000
VERSION     DATAOZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6079031"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VHR64_01870"
     CDS             complement(<1..1325)
                     /locus_tag="VHR64_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX3301597.1"
                     /translation="MRRIQTLLLLALLALMVPMPSAMGQAAGGDLAPTLLSPADGAIG
                     PDSPAFAWQPVDGASGYRIQIAADPGFATILASAETGDVSYQQLAPFGDGAVVYWHVA
                     ALRDGVDPAWSATWSYQVAAPATPEPPTATPQPTSTETATNTPEPTITETPTATSTST
                     VAPTMTSPSTQAPTQTATPKPVVTPVTRSELSGKLGPSAGYPLTGVGRSAGSVHPSVL
                     VDRDLGTIWTSPATTPRSGYVIVALDQVRPIGEIRWLFGRPGRADQIRIAVSTDRASW
                     TEVGRARNATVGDWQSLGVSVRARYVRFTFVNIRGDRWLGGLVEIEVHPPMPKPTPTP
                     PPDPATPPPAPDGIVRNASFEAGRAPWFLDAGTYRSSRLVHGGGLAIALDAAGGFADQ
                     AVLLTPGSSYELSVWGAMGGTWDVGYAGVVFRDANGVRLTELEPPMIEFT"
     gene            complement(1471..>2062)
                     /locus_tag="VHR64_01875"
     CDS             complement(1471..>2062)
                     /locus_tag="VHR64_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301598.1"
                     /translation="VEDYERERERHTFLREQLDDVEGAAAAIRQMLDDVRQTMHERFE
                     ETFSRVSVVFSQMFSTLFGGGSARLVMVADEDGDKTGGIDIVAQPPGKRLQSLALLSG
                     GERALTGVALLFAILKVNPTPFCLLDEVDAALDEANVVRFRDQLTQLANETQAIIITH
                     NRGTIEVADTLYGVSMREDGTSTVLSLRLSDVVLAD"
BASE COUNT          347 a          651 c          733 g          331 t
ORIGIN      
        1 gtgaactcga tcatcggcgg ctcgagctcg gtcaagcgga cgccgttggc atcccggaag
       61 acgaccccgg catagccgac atcccacgtc ccgcccatcg ctccccagac ggacaattcg
      121 tacgaggaac cgggtgtgag caaaacagcc tgatcggcga agccaccagc ggcatcgagc
      181 gcgatcgcca gcccgccgcc gtggaccagg cgcgacgagc gatatgtgcc ggcatcgagg
      241 aaccacggtg cccggcctgc ctcgaagctg gcgttgcgga cgatcccatc gggcgccggc
      301 ggcggtgtcg ccgggtcggg cggtggcgtc ggggtcggtt tcggcatcgg tgggtggact
      361 tcgatctcga ccagaccgcc gagccagcga tcgccgcgaa tattgacgaa cgtgaagcgg
      421 acgtagcgcg ccctgaccga gacacccagc gactgccagt cgccgacagt ggcattgcgc
      481 gcccggccga cttcggtcca cgaggcgcga tcggtcgaga cagcgatccg gatctggtcg
      541 gcgcggccgg gcctgccgaa gagccagcgg atctcaccga tcggccggac ctggtcgagc
      601 gcgacgatca cgtagccgga gcgcggcgtg gtcgccggtg aggtccagat cgtgccgagg
      661 tcgcggtcga cgagcacgga tgggtggacg ctgccagcgc tccgcccgac tccggtcagc
      721 ggatatccag cgctcgggcc aagcttgccg gagagctcgg aacgagtaac cggtgttacg
      781 acaggttttg gcgtcgcggt ctgggtcggt gcctgcgtcg acgggctggt cattgtcggc
      841 gcgactgtcg aggtcgatgt tgcggtcggc gtctctgtga tcgttggctc gggcgtattt
      901 gtcgccgttt cggtggatgt cggctgcgga gtggcggtcg gcggctcggg cgtcgcgggc
      961 gcggcaacct ggtacgacca tgtcgccgac catgccggat caaccccatc tcgcagcgcg
     1021 gcgacgtgcc agtagaccac cgcaccatcc ccgaacgggg cgagctgctg ataactgacg
     1081 tcgccggtct cggcagatgc caggatcgtg gcgaacccgg ggtcggccgc gatctggatc
     1141 cggtatcccg acgcgccatc gaccggctgc caggcgaacg ccggggagtc cggcccaatt
     1201 gcgccatcgg cgggagacag gagcgtcggc gccagatcgc cgcccgcggc ctgacccatc
     1261 gcagatggca tcgggaccat cagcgcgagc aaggcaagca ggagcagggt ctggatgcgg
     1321 cgcatgtcac ctctcgacat cggatcaaca aggagcggct cgatcgctcc agatgtcgag
     1381 gttaggtttg aattcggcgc ggacctatgg gaggaagctg aggaaacggc ccatcaattc
     1441 gggccagacc ggcgtaggac tgtctgccta tcagtcggca aggacaacgt ccgagaggcg
     1501 gagcgagagg accgtgctgg tgccgtcctc gcgcattgag acgccataga gggtatcggc
     1561 tacctcgata gtgccccggt tgtgggtgat gatgatcgcc tgcgtctcgt tggcgagttg
     1621 ggtgagctga tcgcggaagc ggacgacgtt ggcctcatcc agtgcggcgt cgacctcatc
     1681 gagcaggcag aagggcgtcg gattcacttt caggattgcg aagagcagcg ccacgccggt
     1741 cagggctcgc tcgccgccgg agagcagcgc gaggctctgc agccgcttgc cgggcggctg
     1801 ggcgacgatg tcgatcccgc cggtcttgtc gccatcttca tctgcgacca tgaccaggcg
     1861 cgcgctgccg ccgccgaaca gggtcgagaa catctggctg aagacgacag agacgcggct
     1921 gaaggtctcc tcgaaccgct cgtgcatggt ctggcggaca tcatcgagca tctgccggat
     1981 tgccgcggcc gcgccttcga catcgtcgag ttgctcgcgc aggaacgtgt gccgctcgcg
     2041 ctcgcgctcg tagtcctcga cc
//
LOCUS       DATAOZ010000067         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_555674, whole genome shotgun sequence.
ACCESSION   DATAOZ010000067 DATAOZ010000000
VERSION     DATAOZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555674"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            457..807
                     /locus_tag="VHR64_01880"
     CDS             457..807
                     /locus_tag="VHR64_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX3301599.1"
                     /translation="MRDQVLDRPRSFLQRDVVAPASIRTSRRSRKRLALAGAALLTAA
                     GAGWYGYDWWAVGRFVESTDDAYVGGDVTVIAPKVAGFIAEVAVADNQEVHAGDLLVK
                     LTSGASRVPGLPRR"
     gene            complement(901..>2061)
                     /locus_tag="VHR64_01885"
     CDS             complement(901..>2061)
                     /locus_tag="VHR64_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301600.1"
                     /translation="MIDREDTTVRLAAILALVAAAAGPLLTSAAPRAVAADVLTFQVD
                     PAWPKPLPNDWIMGQAAGVAVDAQDNIWVVQRPKTLTDDEKAASFDPPRTKCCKPAPP
                     VLVFNQAGDLIKSWGGPGQGYDWPQNEHGIFVDHKGFVWLAGNGENDGQILKFTAEGK
                     FILQIGKQGRQTNSQDTSRLGKPAQVTVDPETNEVYVADGYYNHRIIVFDGETGAFKR
                     MWGAYGKPPTDENLGPYDPAAAPAQQFRNPVHCVQIARGGLVYVCDRTNDRVQVFRKD
                     GTFVNEYFVEKNTLANGSVWELAIWPDEKETFLLNADGANNEVRTLNRDTGEIEGRFG
                     RNGRMAGDFHWVHNLAIDSKGNVFTTEVDTGKRAQKFLFRGDAALHKRTIVQ"
BASE COUNT          330 a          668 c          659 g          404 t
ORIGIN      
        1 cccgctgcct cgcgcagttc agcggccgaa acatccgacc gcgtaatcgc aaccgccatt
       61 gctctccccc ctccttcaaa gaggagattg cgaatcacac ccggccgagc cgctccaatt
      121 cacaggcgag tcatccgtcc aggcggctgg tatcagtccg tagaaggcgt cccgcaggat
      181 caggatgctc caccattccc cgacccgctc cagactgcgg gcgatcgggc atggcatgtc
      241 gccgaagctc tttcgttgca tgtgctcgat gatgggtctg tagctagcaa aatgcaagtc
      301 tctgccccgg gcttctcgcc cccatgcgaa gaccccgggc ctgccctgat gagggtctac
      361 attgtcgctt gcatattaat agtggcgcgg gtatggcttg catgttgata gtaaccttgg
      421 acccaatccc cggagccttg gagcaaggat tgagtgatgc gcgatcaggt gctcgaccgg
      481 cctcggtcct tcttgcagcg ggacgtcgtg gcacccgcgt ccatcaggac ctcccggcgc
      541 tcacgcaagc ggctggcgct cgccggggcc gcccttctga ccgccgcggg cgccgggtgg
      601 tacggctacg attggtgggc cgtcgggcgc ttcgtcgaga gcacggacga cgcctatgtg
      661 ggcggcgacg taacggtgat cgcgccgaag gtcgccggct tcatcgccga ggtcgcggtc
      721 gccgataacc aggaagtcca tgccggcgac ctgctcgtga agctcacctc gggcgcgtca
      781 agggttcccg gtttgccgcg ccggtgacct ggaaggaggt cgaggccggg acggcgcctg
      841 acgcgtactc gatgcagcga ccgccgcgtc ggacctaagc tctacgcgct gggcgggcca
      901 tcactgcact atcgtgcgct tgtgaagcgc ggcgtcaccc cggaatagaa acttttgcgc
      961 ccgcttgccg gtgtcgacct cggtggtgaa cacgttcccc ttggagtcaa ttgcgagatt
     1021 gtgcacccag tggaagtcac ccgccatgcg gccattgcga ccgaagcggc cctcgatctc
     1081 gcccgtgtcg cggttcagcg tacggacctc gttgttggct ccgtcggcgt tgaggaggaa
     1141 ggtctccttt tcgtcgggcc agatcgcgag ttcccacacg gagccgttcg ctagggtgtt
     1201 cttctcgacg aagtattcgt tcacgaaagt gccgtccttg cggaacacct ggacgcggtc
     1261 gttcgtgcgg tcgcacacat aaacgagccc gccacgcgcg atctggacgc aatggaccgg
     1321 gttgcgaaac tgctgcgccg gggcagcagc cggatcgtag gggccgagat tctcgtcggt
     1381 gggcggcttg ccgtaggcgc cccacatccg cttgaaggcg cccgtttcgc cgtcgaacac
     1441 aatgatgcgg tggttatagt agccatcggc gacatagacc tcgttggtct cggggtcgac
     1501 cgtcacctgg gccggcttgc cgaggcggct cgtatcctgg ctgttcgtct gccggccttg
     1561 cttaccgatc tgcagaatga atttcccctc ggccgtgaat ttcaggattt ggccgtcatt
     1621 ctcgccattg ccggcgagcc acacgaagcc cttgtggtcg acgaagatgc cgtgctcgtt
     1681 ctgcggccag tcgtaaccct gtcccggccc gccccatgac ttgatcaggt cgccggcctg
     1741 gttgaacacg agcaccggcg gggctggctt gcagcatttc gtccgtggcg ggtcgaagct
     1801 cgcggccttc tcgtcatcgg tgagggtttt cgggcgctgg acgacccaga tgttgtcctg
     1861 cgcgtcgacg gccaccccag ccgcctggcc catgatccag tcgttcggca gcggcttcgg
     1921 ccaagcagga tcaacctgga aagtcagcac atctgccgcg acggcgcgcg gcgctgcgct
     1981 ggtaagcagc gggcccgccg cggcggctac gagcgcaaga atggcagcga gcctcacggt
     2041 cgtgtcctcc cgatctatca t
//
LOCUS       DATAOZ010000068         5047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7395437, whole genome shotgun sequence.
ACCESSION   DATAOZ010000068 DATAOZ010000000
VERSION     DATAOZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7395437"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VHR64_01890"
     CDS             <1..1333
                     /locus_tag="VHR64_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301601.1"
                     /translation="YRWLRFVHDANCNITVTKVTLSPQGDDDIWTTTDELQALGYGRD
                     DRKYMVFLDKNEYYLCGQGLTYTDDSVGQSNFNNIYTSWAVIYNGCWSDGTTAAHELG
                     HALGAVQDSAPNASGYGHCTDEWDVMCYADGTPLPMRTVCGDYSHDARLDCGHNDYFH
                     TNPPSGNYLATHWNVANSRFLGKTRPSTSRITLDKSSSKYNGKITVTLSGFSPGYYIN
                     IKWPDQTLLAQVKADVNGNATTMFRTPLVPLGNYTVRASDSSGNWATTTLRVIPRIKL
                     TETSGLAGSTIRVYFYGFAPGDRVEVQWYTTSTSYVVLRTLTVADTGRASSLITIPAN
                     ATTGSHTIRGKVIGVSRSASTNFTVTGPSAAEQPTATPSPTPSPTETTTPEPTATPEP
                     PIETPTPEPTIDPPTATPDPGTPEPPVETPTPEPTSTPDGVETPSSGTDVG"
     gene            1574..3886
                     /locus_tag="VHR64_01895"
     CDS             1574..3886
                     /locus_tag="VHR64_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301602.1"
                     /translation="MAFLHLDRRLVAGFAFAVLALALSLTFSLKQADAHAGHDHGGAL
                     YAQDAPETCDPVDGLCSHGPDPAPPGVDPSVPDQPADFQAASFAICDGNGVSGKRVQV
                     MYVHASDKPDRYAEFVDSFRGWAAEGDAVYQNSAAETGGMRYIRFVHDADCRITVLNV
                     TVSPSGDDDFWSLTSELKWTKGYTDPNRKYLVFLDRNHPDICGQGDVISWRNDPSADD
                     PSPATNPNNNTYGFAVIYNGCWSSNVTIAHELGHNFGAVQGNSPNASRWDHCVDEWDV
                     MCYDDTSGIPLRFECPDYSHNERLDCNHDDYFSTNPKPGSYLATHWNIANSSWLGTSG
                     ARLSFDKEKSKYNGWINASMSGFSPGQQVTLYWPDLTVIAQVTADSSGNATASFRTPL
                     VALGTYTIRASDPAKTTATSLLRVIPRINLNEFSGIQGESNIRVYYYGYAPGDQVELR
                     WYSLDGSTYQVVSTVTIADNGRGTGLFMVPYGTEPGVHTLRGSVVGVGRSTSASFTVT
                     ASPWQLSLSKTSSKFNGLVDATLTGFTPNDTVTLWWPDGLVLGQATTDGSGNAVISFR
                     TPLYPLGNYTVTATDSAGNIAGATLRVIPRIKLTEYSGEAGSRIRVYFYGFSPGEQVE
                     IKWYELDGTTFEVLKTVTIADNGRASALIDIPEDAATGKHMIRGDVIGVSRSASDSFT
                     VTGPGASENPTETPTPSPTETATPEPGGSPTPDASPIVEEPPVETPTPVETSTPEPTE
                     APTDTPTPEPSPTPVPTTEQAAIPRPVEYR"
     gene            complement(4179..>5047)
                     /locus_tag="VHR64_01900"
     CDS             complement(4179..>5047)
                     /locus_tag="VHR64_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3301603.1"
                     /translation="VTVGAAGALFLTATALLSPGDRLVVARPNYASNLETPRAICADI
                     AYLELAFDTGYQIDLDRLAALITPDTRLVSLTSPHNPTGAVIPQSDLAEIVRMVERAG
                     TWLLLDETYRELGDEIHPPASVLSDRVISVSSLSKTYGVPGIRVGWVTCRNRDLMTAL
                     LAAQEQAALAHSILDIAVAEHVLANRDRLLPEIRTHIASQRQIMSNWIAGDDRVEWVE
                     PKGGVVCFPRFADPAIDARAIYTRLKEVGIFLGPGWWFEQEPRHMRLGFGYPTPEELT
                     TALTEISDALSS"
BASE COUNT          944 a         1559 c         1732 g          812 t
ORIGIN      
        1 gtatcgctgg ctccgcttcg tgcacgacgc aaactgcaac atcacggtca cgaaggtgac
       61 gttgagccca cagggcgatg acgacatctg gacgacgacc gacgagctgc aggcgctcgg
      121 ctacgggcgg gacgaccgga agtacatggt cttcctcgac aagaacgagt actacctgtg
      181 cggccagggg ctgacctaca ccgacgattc cgtagggcaa agcaacttca acaacatcta
      241 cacaagctgg gcggtcatct acaacggctg ctggtctgat ggcacgacgg cggcgcacga
      301 gctgggccat gcgctcggcg cggtgcagga ttcggcgccg aatgcgagcg gatacgggca
      361 ctgcaccgac gaatgggacg tgatgtgcta cgcggacggc acgccgctgc cgatgcggac
      421 cgtctgcggc gattacagcc atgacgcgcg gctcgattgc ggccacaacg actatttcca
      481 caccaaccca ccgagcggaa attacctggc aacgcactgg aatgtggcga acagccggtt
      541 cctcggcaag accaggccgt ccacctcgag gatcacgctc gacaagagct cgagcaagta
      601 caacggcaag atcaccgtga ccctctcggg cttctcgccg ggctactaca tcaacatcaa
      661 atggccggat cagacgctac tggcccaggt caaagcggat gtgaacggaa acgcaaccac
      721 gatgttccgg accccgctcg tgccgctggg caactacacc gtgcgcgcct cggattcgtc
      781 cggcaactgg gccacgacca ccttacgggt gatcccgcgg atcaagctga cggagacatc
      841 gggtcttgcg gggtcgacga ttcgggtcta tttctacggg ttcgcgccgg gagatcgggt
      901 cgaggtccag tggtacacga ccagcacgtc gtatgtcgtg ctcaggaccc tgaccgtggc
      961 ggatacgggg cgggcgagct cgctgatcac gatcccggcc aatgcgacga ctggctcgca
     1021 taccatccgg ggcaaggtga tcggggtgag ccgcagcgcc tcgaccaact tcaccgtgac
     1081 cgggccgagc gcggccgagc agccgacggc caccccaagc ccgacgccga gtccgaccga
     1141 aacgactacc cccgagccga cagcgacacc cgaaccgccg atcgagacgc cgacaccgga
     1201 gccgaccatc gatccgccga cggcgacgcc ggatccgggt acgccggagc cgcccgttga
     1261 gacgccgacg cccgagccta cgtcgactcc ggatggggtt gaaaccccca gctcaggaac
     1321 ggacgttggc tgacgccgac tggcatctgg aataattacc atacaagcga aacgcggggt
     1381 acatcgcctc gcgtttcagt tttggagacg gctcgtttag ggggtgtacg tacactctca
     1441 tatggggtac gatctcttcg gtttcacaat cagacgcggg ctggttgcgc gttggtctga
     1501 tcctcattcc gggtacctgg gttctcgcct tgcggagatc ggccgcgcgc tcccctcaaa
     1561 gggtgtgtgg gtagtggcgt ttttgcatct cgatcgacgg ctggtggctg ggtttgcgtt
     1621 cgcggttctc gcgctggctc tctccctcac gttcagtctc aaacaggccg atgcccacgc
     1681 cgggcatgac catggcgggg cgctctatgc ccaggacgcg cccgagacgt gcgatcccgt
     1741 cgatggtctc tgctcgcacg ggccggatcc ggcgccaccg ggcgtcgatc cgtcggtgcc
     1801 ggatcagccg gccgatttcc aggcggcgtc gttcgcgatc tgcgatggca acggcgtcag
     1861 cggcaagcgc gtccaggtga tgtatgtcca tgcgtcggac aagccggacc gatacgcgga
     1921 gttcgtggat tcgttccggg gctgggccgc cgagggcgac gcggtctacc agaacagcgc
     1981 cgcggagacg ggcgggatgc ggtacatccg gttcgtgcac gatgccgatt gccggatcac
     2041 ggtcctgaac gtgaccgtct cgccgagcgg tgacgacgat ttctggagtc tgaccagcga
     2101 gctgaagtgg acaaagggct acaccgatcc gaaccgcaag tacctcgtct tcctcgaccg
     2161 aaaccatccc gatatctgcg gccagggcga cgtgatttcc tggcgaaatg acccgagcgc
     2221 agacgatccg agcccggcga ccaacccgaa caacaacacc tatggcttcg ccgtgatcta
     2281 caacggctgc tggtcgagca acgtgacgat cgcgcatgag ctgggccaca acttcggggc
     2341 tgtacagggg aattctccga atgcgagccg atgggaccac tgcgtcgatg agtgggacgt
     2401 gatgtgctac gacgacacat ccggcatccc gctgcggttc gagtgcccgg attacagcca
     2461 caacgagcgg ctggactgta accacgacga ctacttcagc accaatccga aacccggcag
     2521 ctacctggct acacactgga acatcgccaa cagctcatgg ctgggaacga gcggggcgcg
     2581 gctctccttc gacaaggaga agagcaaata caacggctgg atcaacgcct cgatgagcgg
     2641 gttcagtccg ggccagcagg tcacgctcta ctggccggat ctgaccgtga tcgcgcaggt
     2701 gacggccgat tcatcgggca acgccacggc cagtttccgg accccgctgg tcgcgctcgg
     2761 gacgtacacg atccgggcgt cggacccggc caagacgacc gcgaccagcc tgctgcgggt
     2821 gattccgcgg atcaacctga acgagttctc gggaattcag ggtgaatcca acattcgcgt
     2881 ctactactac gggtatgcgc cgggcgacca ggtcgagctg cggtggtact cattggacgg
     2941 atcgacgtac caggtcgtga gcacagtgac catcgccgac aacggccggg gcaccgggtt
     3001 gttcatggtc ccctacggca ccgagccggg agtccatacc ctgcggggca gcgtggttgg
     3061 cgtcggccgg agcacatcgg cgtcgttcac cgtgaccgcg tcgccgtggc aactctcgct
     3121 gtcgaagaca tcgagcaagt tcaacgggtt ggtcgacgca accctgaccg gcttcacgcc
     3181 gaacgacacg gtgacgctgt ggtggccgga tgggctggtg ctgggccagg cgacgaccga
     3241 tgggtccggg aacgcggtga tctcgttccg gacgccgctc tatccgctcg ggaactacac
     3301 ggtgaccgcg acagattcgg ccgggaacat tgccggggcg acgctgcggg tgatcccgcg
     3361 gatcaagctg accgagtact cgggcgaggc cgggagccgg attcgggtct acttctacgg
     3421 cttctcgccg ggcgagcagg tcgagatcaa gtggtatgag ctggacggga cgacgttcga
     3481 ggtgctcaag actgtgacga tcgccgacaa cgggcgggcg tcggcactga tcgatatccc
     3541 ggaggatgcg gcgaccggca agcacatgat ccggggcgac gtgatcgggg tcagccggag
     3601 cgcatcggac tcgttcaccg tgaccgggcc gggcgcgtcg gagaatccga ccgagacgcc
     3661 cacgccgagt ccgactgaga cggcgacgcc ggagccaggc ggatcgccga caccggacgc
     3721 ctctccgatc gtggaggagc cgccggtcga gacgccgact ccggtcgaga cgagcacacc
     3781 ggagccgacg gaagcaccga ccgatacgcc gacgccggag ccgagtccga caccggtgcc
     3841 gaccactgag caagcagcga ttccgaggcc cgtcgaatat cggtgatctc accgatgcgc
     3901 cggcatggcg ctccccgcag gatgaactca tggacccggg cggccgggtt ggaggcagat
     3961 cgggggagcg tcatgttcat gcttcgattg ctcgaagagt tttggacgat cgtcgcggcg
     4021 ttgagccggt gaggtgctcg cccaaacccg tagtggcggg gctccgtacc cgcctggacc
     4081 ctccggccgg attgcggaca tgcaccacac gggacgcgac tggtcggctc catcgaccgg
     4141 gtcgttcggc cactcaaggc gggtacggag ccccgccact acgatgagag cgcgtccgag
     4201 atttcggtga gggcggtcgt gagttcctcg ggggtggggt agccgaagcc gaggcgcatg
     4261 tggcggggtt cttgctcgaa ccaccagccg gggccgagga agatgccgac ttcttttaat
     4321 cgggtgtaga tggcgcgagc gtcgatggcg ggatcggcga agcgcgggaa gcagacgaca
     4381 ccgcccttcg gctcgaccca ttccacacga tcatccccgg caatccaatt cgacatgatc
     4441 tggcgctgcg acgcgatgtg cgtgcggatc tccggcagga ggcggtcgcg gttcgcgagg
     4501 acgtgctcgg cgacggcgat gtcgaggatg gagtgggcga gggcggcctg ttcctgggcg
     4561 gcgaggagcg cggtcatcag gtcgcgattg cggcaggtga cccagccgac gcggatgccc
     4621 gggacgccgt aagttttcga cagggaggag acgctgatca ctcggtcgct cagcaccgag
     4681 gcgggcgggt gaatctcgtc gcccaactcg cggtaggtct cgtcgaggag gagccaggtg
     4741 ccggcgcgct cgaccatgcg cacgatctcg gccaggtcgg attgcgggat gacggcgccg
     4801 gtcgggttgt gcgggctggt caggctgacg agcctggtgt ccggcgtgat gagggcggcg
     4861 aggcggtcga ggtcgatctg gtagccggtg tcgaaggcga gctcgaggta ggcgatgtcg
     4921 gcacagatcg cgcggggcgt ctcgaggttg ctggcgtagt tggggcgggc gacgacgagc
     4981 cgatcaccgg ggctgagcag ggcggtggcg gtcaggaaga gggcgccggc agcgccgacc
     5041 gtgacca
//
LOCUS       DATAOZ010000069         7541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3186343, whole genome shotgun sequence.
ACCESSION   DATAOZ010000069 DATAOZ010000000
VERSION     DATAOZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3186343"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VHR64_01905"
     CDS             complement(<1..306)
                     /locus_tag="VHR64_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301604.1"
                     /translation="MGDRRQIIGIIGAGQLARMLIEAATPLDIEIALLAASPADGAAR
                     IWPETAIGSPDDAETVAAFARACDVVTFDHELVPDPVLSRLESEGVDLAPSADTMRIA
                     "
     gene            467..2014
                     /locus_tag="VHR64_01910"
     CDS             467..2014
                     /locus_tag="VHR64_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301605.1"
                     /translation="MSDAHEIFESDDVEQFDLTCPACGTDLLADATYNDWRVCGRCDR
                     HFWVSARERATMIARTCDVSELPYSEPQVDPVEHHHRLSAADRQGDARERSALSDAVV
                     TARVSFDGGSVIAALIDAVLLPGGLGIVTTDKVIVAVRAAIRERLPMVIVCGGGSQAI
                     PAGLLQGAQSLRLSAAIAELHRARLPLITVLTHPTGGNLLGGIAINADIRLAEPGLDG
                     SATLAPDKVVPRPDLITRVATILDYLRRRGGPDVELAGSSDAASARLATFQDRPAVVI
                     AIQSNEWQSGQHWSIVRRAQRTAANLDLPIVFAVSGATPLSLDAQVEIRDLLLRHRKP
                     VIGIIQGDLTSAHINLLAVDTVIAAADLAIPGSKAKRYTAQEGRAAGMVDVIAGSDLA
                     LPIARAIEESARLTTTHRIERRLRQADRRGAEVPETRELTRLELRDLRDVQATLVRSV
                     EDWRYRFGQREFTLPTIGNFQGLPAFRNITLPKLQVSRTDLTEMRDKWIARRRSGPQD
                     REGGQSK"
     gene            2049..3146
                     /locus_tag="VHR64_01915"
     CDS             2049..3146
                     /locus_tag="VHR64_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301606.1"
                     /translation="MKQVRTVQVGLGLIGGTVIEQIIDNRARWRDEGLDLGVAAILTT
                     GGALISGDDGFNDDQLRTIVARRHAGASLADVSNEVGASGMASADFDLARVRAEHPIV
                     IDAAAGDATSPVADAALKAGGAVVYSNKAPLSRPIRDARALWDAAADGRVRYETTCGA
                     GLPVISTLKSLLATGDRVIEIVGCLSGTLGAIFSDVADGKPMSKAIVAAKAAGYTEPD
                     PRDDLSGLDVARKALILARTIGRELDLTDIAVESLVPESLADCSVATFLERIADHDAA
                     MTGRQAKAAAENATLKYVATVPADGPISVGVTAVPTSTVLGALQGPENIISIRTERYD
                     RYPLTISGPGAGAAVTAAGIIADLLELARAR"
     gene            3153..3920
                     /locus_tag="VHR64_01920"
     CDS             3153..3920
                     /locus_tag="VHR64_01920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3301607.1"
                     /translation="MTETTSPVRVAPIGSLDAIVASLAPDAYYQSPGGRTFLIAASML
                     GGIARGASVLDVSCGIGQAAVDLAEAFGCKVTAFDNFPPYLAFGRQLAVSRNVSKLVS
                     FMELDGDGVRDQFDAAGFDVVLGMGGGLGDVLSGGIAAGLRAANRWLKPGGLLICGDL
                     ISPATVSPLMEIVFEQSLTDERSYLGAVSEAGFAPIFVSRATSADWDVMRGTIARLRE
                     RNLVVGTDDEQRRLRLTEAAQNHPEIAYLNLLARKKA"
     gene            complement(4189..4599)
                     /locus_tag="VHR64_01925"
     CDS             complement(4189..4599)
                     /locus_tag="VHR64_01925"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004036.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HEX3301608.1"
                     /translation="MAYNTLTPDEERVIVYKGTERPFSGEYEEFDEVGTFVCRRCNAP
                     LYESVHKFDARCGWPSFDKTVEGAVHESLDADGRRIEITCENCGGHLGHVFRGEGFTP
                     RNTRHCVNSISMKFVPQGQRLPQVIKEPSVPATP"
     gene            4935..5639
                     /locus_tag="VHR64_01930"
     CDS             4935..5639
                     /locus_tag="VHR64_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX3301609.1"
                     /translation="MFFDPMYFILVGPGILLMLWAQSRVKSAYRKWGNVRNMQGITGA
                     QAARMVLDANGLQSVPIEQEQGELTDHYDPRKRVLRLSQGVYGVPSVAAIAVAAHEAG
                     HAIQHQQGYAPLQLRTAIVPAVSIGSNLGIFVLIGGILLQSTGLAWAGVALFALSTLF
                     ALVTLPVEFDASHRAKAALVRVGLVDGGVAGGEESKGVRSVLDAAAWTYVAGFATSVL
                     TLLYYVMLVTGMRRSD"
     gene            5798..6838
                     /locus_tag="VHR64_01935"
     CDS             5798..6838
                     /locus_tag="VHR64_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3301610.1"
                     /translation="MFGLLAYTLATGVFRDEIRPGERDIAWLLGLVTAALFLISIVLH
                     ELGHAVIAQRNKLRVGSITLFFFGGVAQVEEEPRSAGVEFRVAVGGPIVSLALAIGFW
                     GLSHVDRFGHLLQTALWWLAGINVVLLLFNLVPGFPLDGGRILRSVIWHFNGSEAKAT
                     RIAVRCGQGVSLLLIGYGIYLIATGSLFNGVWMMMLSAFLRNAATSTGAHVLARSVLE
                     QVTAAQAMARELIYVPARTRVQELFEQQTVINPRQTYVVVDDGPLGVISPLRLAFIPR
                     ERWPWTVVTQIMTPWRQLAEITPETTLMNALRMMEAAHCPYAVVRDQDGNVAGILSQD
                     QIAIKLQSAAQG"
     gene            complement(6861..7367)
                     /locus_tag="VHR64_01940"
     CDS             complement(6861..7367)
                     /locus_tag="VHR64_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEX3301611.1"
                     /translation="MRIYMAGAMFTEADKQYNLHLAKRLRSHGFEVYCPNESEPINDK
                     TRTDVTGKMIYDFDIRELEASNVVLFQVSEDSGSNWEAGYMDALSKYVDPQRWYGVMG
                     MATDIRLQSIPNPTQSGVDNQAGYLNQLVVGGLQKSLGIYRTADEAIARLIDIRTTRE
                     RTTEPTRN"
BASE COUNT         1256 a         2363 c         2555 g         1367 t
ORIGIN      
        1 ggcgatccgc atcgtgtcgg ccgacggcgc caggtcgaca ccctcggatt cgagacggga
       61 cagcactggg tccggcacga gctcgtggtc gaacgtgacc acatcgcagg cccgggcaaa
      121 ggcggcaacg gtctcagcgt catccggcga gccgatggca gtttccggcc agatccgggc
      181 ggcgccatca gcgggtgagg cggccagtaa ggcgatctcg atgtcgagcg gcgtggccgc
      241 ctcgatcagc atccgggcca gctgccccgc gccgatgatc ccgatgatct gtcgcctgtc
      301 gcccatgcac ggagcataat cgagttgagg gttgagggtt gagggttgag agaacggaaa
      361 ggtagccagt agtcagtagc cggtagccag caactgctcg gctgctcgcc tcctcgcctg
      421 ctcgcctaga atggcaccca tcaaccgatc acggccggga catagcatga gcgacgcgca
      481 cgaaatattt gaaagcgacg atgttgagca gttcgatctg acgtgtcccg cctgcgggac
      541 agacctcctc gccgacgcga cctacaacga ttggcgcgtc tgcggccgct gcgaccgaca
      601 cttctgggtc tcggcgcggg agcgcgcgac gatgatcgcg cgcacctgcg acgttagtga
      661 gttgccatat tccgagccgc aggtcgaccc ggtcgagcat caccaccgcc tttccgcagc
      721 cgaccgccag ggcgacgcgc gcgagcgatc agccctctcg gacgccgtcg tcacagcgcg
      781 agtctcgttc gatggcggct cggtgatcgc cgccctgatt gacgcggtcc tgctgcctgg
      841 cggtctcggt atcgtcacga ccgacaaggt gatcgtcgcc gtccgggccg cgatccgcga
      901 gcgattgccg atggtgatcg tctgcggtgg cggatcgcag gcgataccgg ccggtttgct
      961 ccagggcgcg cagtcgctcc gattatcggc ggcaatcgct gagctgcatc gcgcccgcct
     1021 gccactgatc accgttctga cgcacccgac gggcggaaat ttgctcggcg gaatcgcgat
     1081 caacgccgat atccggctcg ccgagccggg actcgacggc tccgccacgc ttgctccgga
     1141 caaagtcgtg ccgcgacccg acctgatcac acgagtggcg acaatcctcg actacttgcg
     1201 gcggcgaggc gggcccgatg tcgagctggc gggatccagc gatgcggcat cggcccgctt
     1261 ggcgaccttt caggatcgcc cggccgtcgt gatcgcgatc cagtcgaatg agtggcagag
     1321 cggccagcac tggagcatcg tgcggcgcgc gcagcggacg gcggccaatc ttgacctccc
     1381 gatcgtcttt gcggtctctg gcgcgacgcc gctctcgctc gacgctcagg tcgagattcg
     1441 agatctgttg ctgcgacatc gcaaaccggt gatcgggatc atccagggcg acctgaccag
     1501 cgcgcacatc aacctgcttg ccgtcgacac ggtcatcgcc gcagccgacc tggcgatacc
     1561 cggcagcaag gccaagcgct acaccgcgca ggagggccgc gccgccggca tggtcgatgt
     1621 tatcgcaggt tccgatcttg ctctgccaat cgcccgcgcg atcgaggagt cggcccggct
     1681 gaccacgacg caccggatcg agcggcgatt gcgccaggct gatcgacgcg gcgccgaggt
     1741 tccggagacg cgcgagctga cgcgcctcga gctgcgggac ctgcgcgatg ttcaggccac
     1801 gctggtgcgc tcggtcgagg actggcgata ccggttcggg cagcgtgagt tcacgctgcc
     1861 gacgatcggc aatttccagg gcctgcccgc attccgaaac atcaccctgc cgaagttgca
     1921 ggtgtcgcgg accgacctga ccgagatgcg cgacaaatgg atcgcgcggc gcaggtccgg
     1981 accacaggat cgcgagggcg gccaatcgaa atgaagcgag atcgaacagt ttgccaatgg
     2041 agtgtggggt gaagcaggta cggacggtgc aggtcggtct ggggctgatc ggcggcaccg
     2101 tgatcgagca gatcatcgat aaccgcgcgc gttggcgtga cgaggggttg gatctcggcg
     2161 tcgccgcaat cctgacgacc ggcggcgcgc tgatcagcgg agacgatggc ttcaatgacg
     2221 accagctgcg cacgatcgtc gcccgtcgcc atgccggggc gagtctggct gacgtcagca
     2281 atgaggtcgg cgcgagtggg atggcgagcg cggacttcga cctcgccagg gtccgggccg
     2341 agcatccgat cgtgatagat gcggcagccg gcgacgccac atcgcccgtg gcagacgcag
     2401 cgttgaaggc cggtggtgcg gtggtctact cgaacaaggc gccgctctcg cgcccgatac
     2461 gcgacgcccg ggcactctgg gatgcggcgg ctgacggacg agtcaggtat gagacgacct
     2521 gcggcgcagg attgccggtc atctcgaccc tcaagtcctt gctcgcgacc ggcgaccggg
     2581 tcatcgagat cgtcggctgc ctgtccggta cgcttggcgc gatcttctcc gacgtcgcgg
     2641 acggaaaacc gatgtcgaag gcgatcgtcg cggcgaaggc ggccgggtac acagagccgg
     2701 acccgcgcga cgacctgagc ggtctcgacg tcgcccgcaa ggcgctgatc ctggcccgga
     2761 cgatcggccg cgagctggac ctgaccgata tcgcggtcga gtcgctcgtg ccggagtcgc
     2821 tggccgattg ctcggtcgcg accttccttg agcgaattgc cgatcacgac gcggcgatga
     2881 ccgggcggca ggcgaaggcg gcggcggaaa acgcgacgct caagtacgtt gcgaccgtcc
     2941 cggccgacgg accgatctcg gtcggggtca cggcggttcc gacgagcacg gtgcttggcg
     3001 cgttgcaagg tcccgagaac atcatctcga tccgcaccga gcgatacgac cgctatccgt
     3061 tgaccatctc gggtccgggc gcaggggcgg cggtgaccgc cgccgggata attgccgatt
     3121 tgcttgagct cgcgcgggcg aggtagggac agatgacgga gacaacctca ccggtgcggg
     3181 tcgcaccgat cggatcgctc gatgcaatcg tggcctcgct ggcgccggac gcctattacc
     3241 agtcgccggg cgggcggacg ttcctgatcg ccgcctccat gctcggcggg atcgcgcgcg
     3301 gggcgtcggt gctggacgtg tcgtgcggaa tcgggcaggc ggcggtcgat ctggccgaag
     3361 ccttcggctg caaggtgacc gcgttcgaca acttcccgcc atatctcgcg ttcgggcggc
     3421 agctcgcggt cagccggaac gtgagcaagc tcgtctcatt catggagctc gatggggatg
     3481 gggtgcgcga tcagttcgac gcggccggct tcgacgtcgt gctcggtatg ggcggcggcc
     3541 tgggcgacgt gctgtcgggc gggatcgcgg ccggtctacg tgcggcgaac cgctggctca
     3601 agccgggtgg attgttgatc tgcggcgacc tgatctcgcc ggcgaccgtc tcaccgctga
     3661 tggagatcgt cttcgagcag tcgctgaccg acgagcgctc gtacctcggt gcggtgagcg
     3721 aggcaggatt cgcgccgatc ttcgtgagcc gggcgacatc ggccgactgg gacgtgatgc
     3781 ggggcacgat cgcgcgattg cgggagcgca acctggtggt cggcacggac gacgagcagc
     3841 gccgtctccg cctgaccgaa gccgcccaga accacccgga gatcgcctac ctcaacctcc
     3901 tcgcccgcaa gaaggcttga ccggcgggtt gagggtggag ggttgaggga aggtggccgt
     3961 atcgcggccc gcctctgggc cggcgggact cacaaaacac cacgaaccca tgaaacggag
     4021 cccagacgta gcgcggggtc accacgcccc gcttcgtcca tcaccacaca cggggttgac
     4081 tggggcaacc tgtttcttgg gttggttttc ccatcgagtc ggtcgtcgag ttcgggcggg
     4141 gcatggtaac cccgcgctac gaacttgatg cgaaatcgat ctcagccact acggcgtggc
     4201 gggaacgctg ggctccttga tgacctgggg gaggcgctgg ccctgcggga cgaacttcat
     4261 ggagatggag ttgacgcagt ggcgagtgtt gcgcggggtg aagccttcgc cccggaagac
     4321 gtgaccgagg tgaccgccgc agttctcgca ggtgatctcg atccgacggc catcggcatc
     4381 gaggctctcg tgaacggcgc cctccaccgt cttgtcgaag ctcggccacc cacagcgcgc
     4441 gtcgaacttg tggaccgatt cgtagagcgg agcattgcag cgtcggcaga caaaggtgcc
     4501 gacctcgtcg aattcctcgt actcaccgct gaagggtcgc tccgttccct tgtagacgat
     4561 gacccgctct tcgtccgggg tcagggtgtt gtaagccatc gaactcctcc gttcgtgtgt
     4621 gcactctcgt gaggccgaaa ttgtaggccc ggcgcgccca acggcgtctg cagatcacta
     4681 cgatgccgct caggtattgg ccggacctga ccattctgtt agcttgattt ttggtgtcta
     4741 cagtgtagag ttagaagcat cagggggagt aaccccacct ccgagatcct cggagtggac
     4801 gcacagtcgt cactacgcgt gaaagcgccg gctgtgcgag cgagtccgca aggctcgtcc
     4861 ggtgagaccc tggcgcatcc attgtctgga tgtggccggg tgatacacaa agagccggcc
     4921 acaagggagg accaatgttc ttcgacccaa tgtatttcat cttggtcgga ccaggcattc
     4981 tgctcatgct ctgggcgcag tcccgggtca agagtgccta ccgcaagtgg ggcaatgtcc
     5041 gcaacatgca gggcatcacc ggcgcccagg ctgcgcgtat ggtgctcgat gccaacgggc
     5101 tgcagagcgt cccgatcgag caagagcaag gcgagctgac cgaccattat gacccgcgca
     5161 aacgcgtgct gcggctctcg cagggggtct acggcgtgcc gtccgtggcg gcgatcgctg
     5221 tagcggcgca cgaggcgggc catgcgattc agcaccagca gggatacgca ccgctgcaac
     5281 tccgtactgc gatcgtgccg gcggtcagca tcggctcgaa cctgggcatc ttcgtgctga
     5341 ttggcggcat tctgctgcaa tcgaccgggc tggcctgggc cggtgtcgca ctgttcgcgc
     5401 tctcgacgct gttcgcgctg gtcaccctgc cggtcgagtt cgatgcctcg catcgggcga
     5461 aggctgccct cgtgcgcgtc ggtttggtcg atggcggcgt cgccggcggc gaagagagca
     5521 agggtgtccg ctcagtgctc gacgcggctg cctggaccta cgttgccgga ttcgcgacat
     5581 cggtcctgac attgctctac tacgtcatgc tcgtgaccgg catgcggcgg agcgactagt
     5641 tcagcactcg acgagcggtg ggactgtcca ccgctcacct ctcatacgtg gtctgaccga
     5701 cagaaagtct tgattgagaa cgcgagaagc gcagccaggc gtaccactgg cggtagtcag
     5761 agggataccg atccgcctgc actggaccct gttcccggtg ttcgggttgc tggcatacac
     5821 cctggcgacg ggagtgttcc gggatgagat ccgaccgggg gagcgggaca tcgcctggct
     5881 cctcggcctg gtcaccgccg ccctcttcct gatttcgatc gtgctccatg agctcggcca
     5941 tgcggtgatc gcgcagcgga ataagctccg ggtcgggagc atcacgctct tcttctttgg
     6001 cggcgtcgcg caggtcgagg aggagccgcg ttcagccggc gtcgagttcc gggtcgccgt
     6061 cggcggtccg atcgtgtcgc tggcgctggc gatcggcttc tggggactct cccacgtcga
     6121 tcggttcggc cacctgctcc agaccgcact ctggtggctg gcggggatca atgtggtcct
     6181 cctcctcttt aacctcgtgc ccggcttccc attggacggc ggccgcatcc tccgctccgt
     6241 gatctggcat ttcaatggga gcgaggccaa ggcgaccaga atcgcggttc gctgcgggca
     6301 aggcgtctcg ctgctgctga tcggatacgg gatctacctg atcgcgaccg gctcgctctt
     6361 caatggcgtc tggatgatga tgctttccgc ctttctgcgg aatgccgcga cctcgaccgg
     6421 cgcgcatgtt ctggccagat cggtcctcga gcaggtcacc gcggctcagg cgatggcgcg
     6481 ggagctgatc tacgtcccgg cccggaccag agtgcaggag ctgttcgagc agcagaccgt
     6541 gatcaacccg cgccagacct atgtcgtggt cgacgatggc ccgctcggcg tcatctcgcc
     6601 attgcggctc gccttcatcc cgcgcgagcg gtggccgtgg acggtcgtga cgcagatcat
     6661 gacgccgtgg cggcaacttg ccgagatcac tcctgagacc accctgatga acgcgctccg
     6721 gatgatggaa gccgcgcatt gcccctatgc cgtcgtccgc gatcaggatg gcaacgtggc
     6781 cgggatcctt tcgcaggacc agattgcgat caagctgcag tcggccgcgc aaggctgacc
     6841 gagtttccag tttctcgttt ctagtttctc gttggctcgg tcgtgcgttc gcgggtggtg
     6901 cggatgtcaa tcaggcgggc gatggcctcg tcggccgtgc gatagatgcc gagcgacttc
     6961 tgcagaccgc ccacgacgag ctggttgaga taaccggcct ggttgtcgac gcctgactgg
     7021 gtcgggttcg ggatgctctg gaggcgtata tcggtcgcca tgcccatcac gccgtaccag
     7081 cgttgcgggt caacgtactt cgagagggca tccatatagc cggcttccca gttggatccg
     7141 gagtcctcgc tgacctggaa gaggacgacg ttcgaggcct cgagctcgcg gatatcgaag
     7201 tcgtagatca tcttgccggt cacgtcggtc cgggtcttgt cgttgatcgg ctcgctctcg
     7261 ttcgggcagt agacctcgaa cccgtgcgat cgcagccgct tggccaggtg caggttgtat
     7321 tgtttgtccg cttcggtgaa catcgcgcca gccatataga tacgcatttt gtggccccgt
     7381 gtaagcactt tggtagactg aagtgcgacg atagcacgga cgtgattgga ccgcagaaat
     7441 ggcagaaacg cactcagctg acaacggtct gaacccggtc atgcagccct ccgacgccac
     7501 cggcgagttc gtcgaggcgg aggagctgct ccatggcgac g
//
LOCUS       DATAOZ010000070        12739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2135306, whole genome shotgun sequence.
ACCESSION   DATAOZ010000070 DATAOZ010000000
VERSION     DATAOZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2135306"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VHR64_01945"
     CDS             <1..282
                     /locus_tag="VHR64_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3301612.1"
                     /translation="GGNLAEVLENLAATVRERRRVRGEVHALTTGPRVSSYVLAAIPL
                     LLFIYFIAISSEYRDVMLWTLYGHLLLGLAAVLSTLGFVFSRKVAKVEY"
     gene            362..1276
                     /locus_tag="VHR64_01950"
     CDS             362..1276
                     /locus_tag="VHR64_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX3301613.1"
                     /translation="MVTVLVPATAFLVVFCTVIVLGRRSQRTILQVNRHAIVPAGVGA
                     VAIRRERVPILGTGGTTAAGQWAARMTRKKVRTASGKLLREAGSPMPLATYLLLRVLC
                     TFVIMPAFVIYIWHGMGASLLGIMTCIVGGFTIPQLPLLRIKRKARRRATDIERAMPD
                     ALDLLVVCAEGGLSLDGAIQQVSQRTRGVLADELRRLLQEIGSGMPRRDAFLSLGDRS
                     QSESLKIFTATIIQADKMGMSIANTLRTLADTMRTRRRLAAETQARKAPIKMMPFLVI
                     FMIPSLFIVILGPAVLAIIQFMRDVSKM"
     gene            complement(1377..1922)
                     /locus_tag="VHR64_01955"
     CDS             complement(1377..1922)
                     /locus_tag="VHR64_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301614.1"
                     /translation="MSLSDRYNRGDGRIQSFDKYLTGIGDDLGECWWRSTGIHRSVLT
                     QGLYVVSAWAGAQHFMVTRDPGMLLITCVALLSYMGLGQSRGGLVEQLQAEATGLPKN
                     SLAFLRLFILAVGVLNLASAACAFAASFGSQPVTSGDVAVPLLIGLSLTALQLSDYIR
                     RTNPVTPSGGMRPDPRQSGRG"
     gene            2399..3067
                     /locus_tag="VHR64_01960"
     CDS             2399..3067
                     /locus_tag="VHR64_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3301615.1"
                     /translation="MIDADDTLWENNIYFEQATEAFVDFLDHSSLSRDDVKAVLDEFE
                     RINAREYGYGSAVYTRSLQDCFHRLAEREVDEAAIEVVVQIGRRILEHEMELIPHVET
                     TLTQLGREYILTLCTKGDRDEQQIKIDRSGLVGYFHQVEIMSEKDAAGYQEILGRLGA
                     RPAEACMIGNSPKSDINPPLQLGMAAVFIPHDHTWQLEHQEVDRAHPRLLVVDRFVDL
                     IRHF"
     gene            3164..3862
                     /locus_tag="VHR64_01965"
     CDS             3164..3862
                     /locus_tag="VHR64_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEX3301616.1"
                     /translation="MEQYRSIYPAQSVAQVGAEERSAFLRRVYQHVAGALALFIILEA
                     ILQSLPFSTTVAERMSGAWLLVILGFWGVGWISAKWTMPGLPLSQQYLGLGLFVVAEA
                     VIFMPLIAYVRLYEDPNVLPTAGILTATLVVGLTLIALDSRTDFSLLRGALFIMMPVA
                     FGLIIASILFGIGLGAWFSVLMIGLAGASILYKTDEITKTSRTDGYVSAALALFASFM
                     LLLWYVIRLLSARR"
     gene            complement(3926..4189)
                     /locus_tag="VHR64_01970"
     CDS             complement(3926..4189)
                     /locus_tag="VHR64_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015880938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX3301617.1"
                     /translation="MGLILWLIFGALAGWLASLINGTNREQGWVGNIILGILGALVGG
                     FIYNLIADGSFSAGFNVGSLIVAIIGALIVSWLFSILTGRKAI"
     gene            complement(4316..4774)
                     /locus_tag="VHR64_01975"
     CDS             complement(4316..4774)
                     /locus_tag="VHR64_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEX3301618.1"
                     /translation="MDEIEEFSWIVPERLLACRYPWDRDALERLKALGVRGVVNLTER
                     RHDQAILAELEIFETHLPVPDLTPPSPEDIRTAIEVIDAAHAAGLPVAVHCAAGLGRT
                     GTVMAAWLVRQGFDADSAISRIRELRPGSIETDEQEEAVRDFARRLPDAL"
     gene            5162..5953
                     /locus_tag="VHR64_01980"
     CDS             5162..5953
                     /locus_tag="VHR64_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX3301619.1"
                     /translation="MTFPLVTSETGRPVQCFTPDFYLPDHDVYIEMTTMRQSLVTRKN
                     RKFRLMREIYPHVNVRLLYRKDVELIVERYTRGRDRTDLLPGPILATADQIGHKAAEV
                     AARLECGREPVTLIALGEGAVRFRDRIAEAFTNVGGQADNALVQVKRSRRTSSVCEAE
                     ICLTSADDLRDSRRILVADVVSTGLTVNAAATWFRSAGIPIEGVVALLDRRSARLIDV
                     PLLVPGIPAPSSWMVGAGLGGGHWSRRSDVHSLVQPSRFEDAAKS"
     gene            6124..6558
                     /locus_tag="VHR64_01985"
     CDS             6124..6558
                     /locus_tag="VHR64_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3301620.1"
                     /translation="MNSVLICVRGFRITAVLDTLRKRLHPSMYWVVLHVVDPDTANEP
                     DSAVGRSDQRQHAHDRLAETSHDLHLEVRAEIEQWLAAHGRRAEIVMAEGRPEQEILR
                     VAHDRDVALIAIGDLTPPGPRRLPPIAQFVVDHAECDVLLVR"
     gene            6616..7914
                     /locus_tag="VHR64_01990"
     CDS             6616..7914
                     /locus_tag="VHR64_01990"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3301621.1"
                     /translation="MSSIVEAEAGEEDALIDAAQAGPSPSRFAVFSNRNFTIFWLSLV
                     ITNIGTWMAGVAEGWLITDLEPERKSFFLGLIAIAFAIPMLVLPPFGGIVADRLPKVT
                     GLRLTQVGFLVVNSTVAILALSGRVNVHVLIAASFMGAVVLAFDSPIRHSMVPDLVPR
                     SQLTSAVSINAVAFSGAGLIGPAIGGLLIPLVTPGGVFLINAISSISVIIALQLMKDL
                     PETSRTRVAHEQENPRSALQRAFRHMRSSPLLWGLFLMALVAGFFGRSYTPMLPVMSR
                     DVYGVGAAANGILISAGGLGAMIGGLGLSAFSAELSRRGRLAALMVIGQGALLLLLAS
                     SDSYLVGLATLAIAGALGAAAVALITTLVQEHVPVQFRGRVMGFFLLTMISFPSAGSF
                     LMGVVADRTSIQWALAAYAVVVIAGVAYVIARNPVIAATQ"
     gene            7911..8534
                     /locus_tag="VHR64_01995"
     CDS             7911..8534
                     /locus_tag="VHR64_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301622.1"
                     /translation="MSGRAVEPPWEQPFHWWGPPWAPPNVMSLADLVAQGLIRPEQAA
                     RLTNHVRQGGSLVVAALRSGTGKSTLAHALMAAIEPDRNRIYIRGTYEPFDWLPSAQP
                     DQTTLLINEISDHLPAYCWGGCAKRVLSLARRGYQVIATLHADSPAAFLALLRSPEIG
                     VGEDEIVALDIVVFLDITASGQRSVSDIMQVRSDPDSGRAVLRPIDL"
     gene            8699..10117
                     /locus_tag="VHR64_02000"
     CDS             8699..10117
                     /locus_tag="VHR64_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX3301623.1"
                     /translation="MASTTSSTGDRAATLGELRASGYSPKSIREEMRANLIAKMRSGA
                     DLFPGIHGYETTVVPAIENAILSGHDIIFLGERGQAKTRMARSLTNLLDEYIPIVAGS
                     EITDDPFDPVSRYARDLVEKHGDATPIEWVHRDQRYGEKLATPDTTIADLIGEVDPIK
                     VAEGRYLTDELTISYGLIPRTNRGIFNLNELPDLAERIQVGLLNIMEERDVQIRGYKI
                     RLPLDIYVVASANPEDYTNRGRIITPLKDRYGSQIRTHYPETVELEVEIMEQERHQYE
                     TDDVTVMVPNFMKDVVAELTHQARHSHDVSQRSGVSVRMSVANYENLVSNSIRRAIRL
                     GEKQAAPRVSDLDALYSSTHGKIELETLGEVSETAVFDRMTNSAVLAVFNRTFSIREF
                     DRLVQAFENGLAVETSDLMPSMDYVLQLSHLDGMNDIVDTLGVSGNPAGVASAVEFVL
                     EGLHLNRRLNKATGDGPARYSR"
     gene            10142..12274
                     /locus_tag="VHR64_02005"
     CDS             10142..12274
                     /locus_tag="VHR64_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3301624.1"
                     /translation="MSAHRHRYSRWDGTQQISSISADDIMRSIADELVNDGDLMRALR
                     QLFREGIEMADGERLPGWWEMLQRVRQQRQEQLNRYDLGSIFEDIRNRLQDIVETERE
                     GIDDRVEEAEQRLADAEKSSAPSEQGGQKASGQDSESGESGESGSSGEGGIDIDQERA
                     LTDMLRQMAARKQAQLDALPPDPAGAIKELQQYDFMSPAARQKFQDLLEMLQQQVMNQ
                     MFQGMRQALGDMTPEDISQMRQMMSELNDMIEANNRGEPTNFEEFMHRWGHFFGPGVN
                     SLEELIERMRQQMAAMNQLMESMPGEQRSQLEEMMRNLAQDPGLQEQMARLAENIGQM
                     MPQQGGQRRPYQFSGDESITLSEAVRLMDRLNDYDELERDLKDVRDWQDFAKLDDEKI
                     RDLLGEESHEQMQQLSQLAQMLEDAGYIRKNRKGYELTPQGVRKIGEKALNDIFQELA
                     KDKVGQHELQHGGGPGERIDVTKPYEFGDPFLLDLPQTVMNAVQRGGAGSPVQLKADD
                     FEIYKTEYITRSATVLMIDLSRSMFYNQYFPAAKRIALALDSLIRSKYPRDSLKIIGF
                     SYVATPLKPADLPGLTWNEYEVGTNMQHGFRMAQELLAREHASNKQIIIITDGEPTAH
                     VEPSGEIFFSWPPTPQTWDATLREVLRCTRDGIRINTFMLERSTYLMKFVNDLSKING
                     GRVFFASPDHLGEYVLVDYVRNKRKWIQ"
     gene            12417..>12739
                     /locus_tag="VHR64_02010"
     CDS             12417..>12739
                     /locus_tag="VHR64_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301625.1"
                     /translation="MVGALEQGRTHFDRHEWTEAHAHFAQADHEVPLESADIERLATA
                     AYLIGLDAESSTAWSRAYHEHVSAGDWVGATRCAFWMGFGLMNRGEYAQAGGWLARAR
                     RQLDQT"
BASE COUNT         2407 a         3909 c         3957 g         2466 t
ORIGIN      
        1 ggtggcaatc tagccgaagt gctggagaat cttgctgcga ctgtgcgcga gcgtcgccgg
       61 gtacgtggtg aagtgcacgc actaaccacg gggccacgtg tgtcgagtta tgtcctcgcc
      121 gcaattcctt tactgctctt catctatttc atagctatca gttcggaata tcgcgatgtc
      181 atgctgtgga cgctgtatgg ccatcttctg ctcgggcttg cggcggttct gtccacgctc
      241 ggcttcgtat tcagtcgcaa ggtcgcgaag gtggaatact gatgtcattc ctcagagccg
      301 cttgcgagcc gaaatcgatt tccatgcttc gaccaagatg attggggagg aataggggtc
      361 catggtgaca gtgcttgttc ctgcgacggc attcctggtc gtgttctgta ctgtgatcgt
      421 ccttgggcga cggtcgcaac gaacaatctt gcaagtcaat cgacatgcca ttgtgccggc
      481 aggcgtcggc gcagttgcca tccgtcggga gcgagttccg attcttggca caggcgggac
      541 aactgctgca ggtcagtggg cagcgcgaat gactcggaaa aaggtgcgga cggcatcagg
      601 caagctcctt cgcgaagcgg gcagcccaat gccgctggca acctatcttt tgcttcgggt
      661 cctctgcacc tttgtgataa tgccggcgtt tgtgatctat atctggcacg gaatgggtgc
      721 atccctgctc gggatcatga cctgtattgt gggagggttc accattcctc aactcccact
      781 gctccgaatc aagcggaaag cccgccgccg ggccacggat atagaacggg ccatgccaga
      841 tgcgcttgat ctccttgtag tctgcgctga gggtggactg agcctcgatg gcgcaattca
      901 gcaggtatct cagcgtacca gaggcgtgct tgcggacgag ctacgccggc tgctgcagga
      961 gatcgggagc ggcatgccgc ggcgcgatgc atttctgtcg cttggggacc ggtcccaatc
     1021 cgagagtttg aaaatcttca cggcaaccat cattcaggcc gacaagatgg gcatgagcat
     1081 cgccaacaca ttgcggacgt tggccgacac gatgcgaaca aggcgccgtc tggctgcgga
     1141 gactcaagcc agaaaggcac ctatcaagat gatgccgttc cttgtcatct tcatgatccc
     1201 gtcactcttt atcgtcattc tcgggcctgc ggtcctggcg atcattcaat tcatgcgcga
     1261 cgtttcgaaa atgtgatttg cgcaaggatc gacatcgcgc aagtcggaga tcagcggaaa
     1321 tcatgcgcag agtgatgata gatgctctga gtgggcacca acgtttgtgg aggatgtcag
     1381 cctcggccgg actgccttgg atcagggcgc attccaccgc tgggtgtcac cgggttggtg
     1441 cggcgaatgt agtcgctcag ttgaagcgcg gtcagcgaga gtccgatcag caggggcacc
     1501 gcaacgtccc cggaggtgac tggctgcgat ccaaacgacg ccgcgaatgc acacgccgca
     1561 ctcgcgaggt tgaggacccc cacggccagg ataaacagcc gcaggaaggc cagcgaattc
     1621 ttgggcagac cggttgcctc agcctggagc tgctcgacca ggccgccgcg ggattgaccc
     1681 agacccatgt agctcaggag cgcaacgcag gtgatcagga gcatgcccgg atcgcgggtg
     1741 accatgaagt gctgggcgcc cgcccaggcc gaaacgacgt agaggccctg ggtcagcacc
     1801 gatcggtgga tccccgtgga cctccaccag cactcgccga gatcgtcgcc gatcccggtc
     1861 aagtatttgt cgaatgactg gatgcgcccg tcaccgcgat tgtatcgatc gctaagactc
     1921 acgaaagcgt cctttcaccc attccgcgaa cggtatgcgg gtacgagcgg gcctgtgaat
     1981 ctatgcccgg caggaagcgc cgggacgttg cccgaccggt ctatggcatt ttccgcctga
     2041 ccggatcgag tgcttgtgta ccgcgctacg aaccgaatag ttacccgcag tatcccacaa
     2101 tattcccatt gctggaaggg gcgttgttcg acgtcctgaa aagtgatcgc cacagtggca
     2161 atcgctgcga gtgccgggcg gtatccgtta cccatggaac cgtcgtcaga actgacacgt
     2221 gcggtcgact tgggagcttc ggggacggcc gcagagtcgg agcactccac tgagagccct
     2281 cacttcgggc ggctatactc gtcacttgat tgtcgagcag atcactcatc acgcggacca
     2341 agaaacctct catatgcccg taagcaacaa ccgacgttgg ccggaaatcc ggcatctgtt
     2401 gatcgacgcc gacgacacgc tctgggaaaa caacatctac ttcgagcagg cgacggaagc
     2461 atttgtcgac ttcctcgatc attcctcgct cagtcgggat gacgtgaagg cggtgctcga
     2521 tgaattcgag cggatcaatg cccgggagta tgggtacggt tcagcggtct acacgcggtc
     2581 attacaggac tgcttccacc gattggccga gcgggaggtc gatgaggccg cgatcgaggt
     2641 cgtggtccag atcgggcggc ggattctcga gcatgagatg gaactgatcc cacatgtcga
     2701 gacgacgctg acgcagcttg gccgggagta catcctgacc ctctgcacca agggcgatcg
     2761 cgacgagcag caaatcaaga tcgatcggtc cgggcttgtg gggtatttcc atcaggtcga
     2821 gatcatgtcc gagaaggacg ccgccggcta tcaggagatc ctggggcgtc tgggtgcgcg
     2881 tcccgccgag gcgtgcatga tcggcaactc gccgaagtcg gacatcaatc cgccgcttca
     2941 gctcggcatg gcagccgttt tcatcccgca tgaccacacc tggcagctcg agcatcagga
     3001 ggttgatcgg gctcatccgc ggctcttggt ggtggatcga tttgtagacc tgatccggca
     3061 cttttgaccc aaatgtgcca tcgactgccg catccattcg gagtaggctt tacatatcag
     3121 attgaatcct gtgaagcgtt cgttccggca atggaggctc agaatggaac agtaccgatc
     3181 gatatacccc gcgcagtcag tcgcgcaggt cggggcagag gagcggtcgg cgttcctgcg
     3241 tcgcgtctat cagcatgtgg ccggggcgct tgcgctcttc atcatcctcg aggcgattct
     3301 gcagtcgctg ccgttctcta cgaccgttgc cgagcgcatg agcggcgcct ggctgcttgt
     3361 catcctcgga ttctggggcg tgggctggat ctcggccaag tggacgatgc cgggtctgcc
     3421 gctctcgcaa caatacctcg gccttggcct cttcgtcgtg gccgaagcgg tcatcttcat
     3481 gccgctcatc gcctatgtgc ggctctatga ggatccgaac gttctgccga cggctgggat
     3541 cctgaccgcc acgcttgtcg ttggactgac gctgatcgcg ctcgattccc gcaccgattt
     3601 cagcctgttg cgcggggcac tgttcatcat gatgccggtc gcgttcggac tgatcatcgc
     3661 ctcgattctg ttcgggatcg gacttggcgc ctggttctcg gtgctgatga tcggactcgc
     3721 gggagccagc atcctctaca agaccgatga gatcaccaag acgtcgcgga cagatggcta
     3781 tgtgtcggcc gcgctggcgc tcttcgcctc cttcatgctc ctgctttggt acgtgatccg
     3841 gctgctttcc gcacgccggt agacgaggac aacaaaacgg ccccgggggc taccccgggg
     3901 ccgttttttc gtgaagatgc tgcctctaga tcgccttgcg accggtcaga atgctgaaca
     3961 gccagctgac gatcaaagcg ccgatgatcg cgacgatcag gctgccgacg ttgaaaccgg
     4021 cactaaagct gccatcggcg atcaggttgt agatgaatcc gccaaccagg gcaccgagaa
     4081 tgcccaggat gatattgccg acccatcctt gttcgcggtt tgtgccattg atcaagctcg
     4141 ccaaccatcc agccagcgcg ccaaagatca accaaagaat cagacccata ctgtcactcc
     4201 tttttgcgtt tcctgactgc accgttgcag cggttagccg gaagtaacga tacggacgtc
     4261 tcacggctcg cacagtgcca agcctacaga tctgcccaca tttgggttgg attggctaca
     4321 aagcatcagg aagccggcga gcgaagtccc gaacggcttc ttcctgctcg tcggtttcga
     4381 tcgaaccggg ccgcagctct cgaatccgtg aaatcgcgga gtcggcgtca aacccttgcc
     4441 tgaccagcca ggcggccatg acagttccag tgcgaccgag tcccgcggcg cagtgaaccg
     4501 caaccgggag tccggccgcg tgggcggcgt caatgacctc gatcgcggtc cggatgtctt
     4561 ccggcgaagg cggcgtgagg tccggtacgg gcaaatgcgt ctcgaagatc tcgagttcgg
     4621 ccaggattgc ctgatcgtgg cgcctctccg tgaggttgac gaccccgcgg acaccgagag
     4681 ccttcagccg ctccagggca tcgcggtccc acggatagcg gcaggcgagt aaccgctccg
     4741 gaacaatcca gctgaactcc tcgatctcgt ccatggtgca cctctgacac agtgtacgta
     4801 cgcctaattg cggcaaggtg acctccggcg actgtccgta cagttgtcgc gacggagaac
     4861 ctgtgcaaaa ctctgtatta cccccgaata ctcgcacgaa tcgctgtttg accgttacgg
     4921 aatccggctt gggtgcgagg gggaatcagt ggcagaagcg tggccgagcg tgagcgcggg
     4981 cctggtatcg ttgcaagcta ccactgaacg tacacacgac aacactacga gtttcccgca
     5041 acgacaacag attgtcgacc atgatctgcg ttcggagcag tccgtccgtt ttgcccaccc
     5101 ggccgaagca gtcgttgccg atctgctcag ttcattcgga gtcagatggc tgtacgagcc
     5161 tgtgaccttc cccctcgtta cctctgaaac cggcaggccg gtacagtgct ttacgcccga
     5221 cttctatctg cctgatcacg acgtctacat cgagatgacg accatgcggc agtcactggt
     5281 cacccgcaag aaccgcaagt tccggctcat gcgagagatc tacccgcacg tcaatgttcg
     5341 cctgctctat cgcaaggacg tcgagttgat cgtcgagcgg tacaccaggg gacgggatcg
     5401 caccgatctg ctgcccggac cgatccttgc gacagccgat cagatcggcc acaaggctgc
     5461 agaggtagcc gcccgtctgg agtgcgggcg ggagccggtc actctgatcg cgctcgggga
     5521 aggcgcggtg cgctttcgcg accggatcgc ggaggctttc acgaacgtgg gtggacaggc
     5581 agacaacgcg ctcgttcagg tcaagcggtc cagacgaacg tcgtctgttt gtgaggcgga
     5641 gatttgcctt accagtgcag atgacctccg ggacagccgg cgcattctcg ttgcggatgt
     5701 ggtcagtacc ggtctcacgg tcaacgcggc agccacgtgg tttcgctccg ccggcatccc
     5761 gatcgaggga gtggtcgcac tgctcgaccg ccgctccgcc cggttgatcg atgtgccgct
     5821 cctggtgccc gggatacctg cgccatcgtc atggatggtc ggcgcgggcc tcggtggggg
     5881 ccactggtcg agacgctccg atgtccattc gttggttcag ccgagtcgat tcgaagacgc
     5941 tgccaagtcc tgacgcgcga acgcattcgg cgggctgagt tagaggcgtg cgccgttcca
     6001 attctgcccg gccgccacat tgggccgaca atgacgacgt atggttgtct ggcacgacct
     6061 gcgtgtcttg ctcggtccgc gcaggttgat cctgtcgatc gtcaaccggt ggagcgttgt
     6121 gcgatgaaca gtgtcctcat ctgcgttcgt ggcttccgga tcactgccgt gctggacacc
     6181 ttacggaagc gattgcaccc ttcgatgtac tgggtggtgc tgcacgtggt tgacccggat
     6241 actgccaacg agccagacag tgccgtcggt cgtagcgacc agcgccagca cgctcatgac
     6301 cgcctggccg agacatccca tgaccttcac ctcgaggttc gcgcggagat cgagcagtgg
     6361 cttgccgcgc atggccggcg ggcagagatc gtgatggccg aagggcgccc ggagcaagag
     6421 attctgcgcg tcgcgcatga ccgggatgtg gcgctgatcg cgatcggtga cctcacgccg
     6481 ccgggacctc gccggctgcc gccaatagcg cagttcgtgg tcgatcacgc cgaatgcgac
     6541 gtgttgctcg tccgatagtt cacctggcaa tccacagtcc gcaagatcgc tccgacccgg
     6601 tggtatgctg cgcgaatgtc gagcatcgtt gaagccgaag ccggggaaga agacgccctc
     6661 atcgatgccg cccaggctgg tccgagtccc agtcgattcg ccgttttctc caatcgcaac
     6721 ttcacgatct tttggctgag cctggtcatc accaacatcg gcacctggat ggccggcgtc
     6781 gccgaagggt ggctgatcac agacctcgag ccggagcgca aatccttctt cctcggcctg
     6841 attgcgatcg cgttcgcgat tccgatgctg gtcctgccgc cgttcggcgg gatcgtggcg
     6901 gatcgcctcc cgaaggtcac gggacttcgc ctgacccagg tcgggttcct ggtcgtcaat
     6961 tccacggtag cgattctggc gctgagtggc cgggtcaatg tccacgtgct gatcgcggcc
     7021 tcgttcatgg gtgcggtggt gctggccttc gattcgccga ttcgccactc gatggtgccc
     7081 gatcttgtac cgcgttcgca gctcacttcc gcggtctcga tcaacgccgt ggccttcagc
     7141 ggcgccggac ttatcggacc ggcgatcggc ggtctgctga tcccgttggt taccccgggc
     7201 ggcgtcttcc tgatcaatgc catttcgtcg atctcggtaa tcatcgcctt gcagctcatg
     7261 aaggacctgc cggagacgag ccgaacccgg gtcgcgcacg agcaagagaa cccgcggagt
     7321 gcgctgcaac gggcgtttcg gcacatgcgt tcctcgccgc tgctctgggg actcttcctg
     7381 atggcgctcg ttgcggggtt cttcgggcgg tcgtatacgc cgatgctgcc ggtgatgtcc
     7441 cgggatgtct acggcgtcgg cgctgccgcc aacggaatcc tgatctctgc cggcgggctc
     7501 ggggcaatga tcggcggcct cgggctgagc gcattctctg ccgaactgtc gcggcgggga
     7561 cgtctggcgg ctttgatggt catcggccag ggcgcgctcc tgctcctgct ggcctccagc
     7621 gatagctacc tggtcggtct cgcgacactc gcgatcgccg gggcgctggg cgcggcagcg
     7681 gtggccctga tcacgacgct ggtccaggag cacgtgccgg tgcagtttcg cggacgggtg
     7741 atggggttct tcctgctgac catgatcagc ttcccgtcag ccggatcgtt cctgatggga
     7801 gtcgtcgcag accggacttc gatccagtgg gcattggcgg cctacgccgt cgtcgttatc
     7861 gccggggtcg catacgtaat tgctcgcaac ccggtgatcg ccgccacgca atgagtggtc
     7921 gagcggttga gccgccgtgg gagcagccat tccattggtg gggtccgcca tgggcgccgc
     7981 cgaacgtgat gagcctcgct gacctggtgg cgcaggggct gatccggccg gagcaggccg
     8041 cgcgactgac caaccacgtt cggcagggcg gttcacttgt cgtcgcggcg cttcgatccg
     8101 gcacgggcaa gtcgaccctg gcgcacgcgc tcatggccgc gatcgagcct gaccggaacc
     8161 ggatctacat ccgcgggacc tacgagcctt tcgactggct cccatcggcc caaccggatc
     8221 agacgacact tctcatcaac gagatcagtg atcacctgcc ggcgtactgc tggggtggct
     8281 gcgcaaaacg ggtactttcc ctcgctcgtc gcggctacca ggtcatcgcc acactccatg
     8341 ccgactcgcc agcggcgttc ctggcgctcc tgcggtcgcc cgaaatcgga gttggcgagg
     8401 acgagattgt cgcactcgac attgtcgtat tcctcgacat cacggcgtcc gggcagcggt
     8461 ccgtgtccga tatcatgcag gtcagatcag acccggattc tggacgcgcg gttctgcgtc
     8521 cgatcgatct ctgatctcgc aaacctgcca gtgtggtcag gttgtgcaac cgggggtcga
     8581 cgttatgcgt accagagggg taggatgcag ttctatactc aatcgttgaa cgcgcccact
     8641 gctgcgcgcc ttccgctgaa ttgacccatc cacatctgac aatatccagg gagctgccat
     8701 ggccagcacg acatcttcca ccggcgatcg agcggcgacg ctcggtgaac tccgcgcatc
     8761 cggatacagc ccgaaatcga tccgcgagga gatgcgcgcg aatctgatcg cgaagatgcg
     8821 atcaggcgcc gacctgttcc ccggcattca cggctacgag accacggttg taccggccat
     8881 cgagaacgcg attctttccg ggcacgacat catcttcctg ggcgagcgcg gccaggcaaa
     8941 gacccgcatg gcccggagcc tgaccaacct gctcgatgag tacatcccga tcgtggccgg
     9001 ctccgagatc accgacgacc cgtttgaccc ggtctcccgg tacgcccgcg acctggtcga
     9061 gaagcatggc gacgccacgc ccatcgaatg ggtccaccgc gaccagcggt acggcgaaaa
     9121 gctcgcgacc cctgacacga ccattgccga tctgatcggc gaggtcgacc cgatcaaggt
     9181 tgccgaaggt cgctacctga ccgacgagct gaccatctcc tacggactga tcccgcgcac
     9241 gaatcgcggc atcttcaacc tgaacgagtt gccggatctg gccgaacgga tccaggtcgg
     9301 cctgctcaac atcatggaag agcgcgacgt ccagatccgc ggctacaaga tacgtctgcc
     9361 gctcgatatc tacgtcgttg cgtcggcgaa cccggaggac tacacgaacc ggggccggat
     9421 catcacgccg cttaaggaca gatacggctc ccagatccgc acgcactatc cggagaccgt
     9481 cgagctcgaa gtcgagatca tggaacagga gcggcatcaa tacgagaccg atgacgtgac
     9541 cgtgatggtt ccgaacttca tgaaggacgt cgtcgctgag ttgacgcacc aggcccgcca
     9601 ctcgcacgac gtctcccagc gatcgggtgt ctcggtccgc atgtcggtcg ccaactacga
     9661 gaacctcgtc tcgaactcga ttcggcgagc gatccggctc ggcgagaagc aggccgcgcc
     9721 gcgcgtcagc gatctcgacg ccctctactc gtcgacccac ggcaagatcg agctggagac
     9781 actgggcgag gtgagcgaga cggccgtctt cgaccggatg accaacagcg ccgtgctcgc
     9841 cgtgttcaat cgcaccttct cgatccgcga gttcgaccgg ttggtccagg ccttcgagaa
     9901 cggcctggct gtcgagacgt cggacctgat gccatcgatg gattacgtgc tgcagctcag
     9961 ccatctggac gggatgaacg acatcgtcga cacgctcggc gtcagtggca accccgctgg
    10021 tgtagcttcg gccgtcgaat tcgtgctcga gggcttgcat ctgaatcgtc gcttgaacaa
    10081 ggccacgggg gatggcccgg cgcgctacag ccgctaggca cctgcccgga aggagggcga
    10141 gatgagcgcc catcgccacc gctatagtcg ctgggatggt acccagcaga tcagttccat
    10201 ctcggcggac gacatcatgc ggtcgatcgc cgacgagctc gtcaatgacg gcgatctgat
    10261 gcgcgcgctc cgccaactct tccgcgaagg gatcgagatg gcagatggcg agcgcctgcc
    10321 cggctggtgg gagatgctcc agcgggtgcg gcagcagcgc caggagcagc tcaaccgcta
    10381 cgacctcggc tcgatcttcg aggacatccg caatcgcctg caggacatcg tcgagacgga
    10441 gcgcgaaggc atcgatgatc gcgtcgaaga ggccgagcag cgactcgccg acgccgagaa
    10501 gagctccgct ccatcagaac aaggcgggca aaaagcgtca ggtcaggact ctgaatcagg
    10561 tgagtccggc gaatccggca gctccggcga gggcgggatc gacatcgacc aggagcgcgc
    10621 gttgaccgac atgctccgcc agatggccgc ccgcaagcag gcgcagctcg acgctctgcc
    10681 gccggatccg gccggggcga tcaaggagct tcaacagtac gacttcatga gcccggccgc
    10741 ccggcagaag ttccaggacc tgctcgaaat gctccagcag caggtcatga accagatgtt
    10801 ccaggggatg cggcaggcac tcggcgacat gacgcccgag gacatctccc agatgcgcca
    10861 gatgatgtcg gaactcaacg acatgatcga agcgaacaac cggggcgagc cgacgaactt
    10921 cgaggagttc atgcatcgct ggggccattt tttcggcccg ggcgtcaatt cgctcgagga
    10981 gctgatcgag cggatgcgcc agcagatggc ggcaatgaac cagctcatgg agagcatgcc
    11041 cggcgaacag cgctcccagc tcgaggaaat gatgcgcaac ctggcgcagg acccgggctt
    11101 gcaggagcag atggcgcgat tggccgaaaa catcggccag atgatgcctc agcagggtgg
    11161 ccagcgccgc ccctaccagt tcagcggaga cgagtcgatc acgctcagcg aggcggtccg
    11221 gctcatggac cggctcaacg actacgacga actcgagcgc gatctcaagg acgtgcgtga
    11281 ctggcaggac ttcgccaagc tcgacgatga gaagatccgt gacctgctcg gcgaggagtc
    11341 gcacgagcag atgcagcagc tctcgcagct cgcccagatg ctcgaggatg ccggctacat
    11401 ccgcaagaac cggaaggggt atgagctcac gccgcagggt gtccgcaaga tcggcgagaa
    11461 ggcgctgaac gacatcttcc aggagcttgc caaggacaag gtcggccagc acgagctgca
    11521 gcacggcggc ggccctggcg agcggatcga tgtcacgaaa ccatacgagt tcggtgatcc
    11581 gttcctgctg gatctcccgc agaccgtcat gaacgcggtg caacggggtg gggctggatc
    11641 gcccgttcaa ctcaaggcgg atgacttcga gatttacaag accgagtaca tcacgcggtc
    11701 ggccacggtc ttgatgatcg atctgagccg ctcgatgttc tacaaccagt actttcccgc
    11761 cgcgaagcgg atcgcgctcg cgctcgactc gctgatccgg tccaagtacc cgcgcgactc
    11821 gctcaagatc atcggcttct catatgtcgc cacgccgctc aagccggccg atctccccgg
    11881 gctgacctgg aacgagtacg aggtcgggac caacatgcag cacggcttcc ggatggcgca
    11941 ggagttgctg gcgcgcgagc acgccagcaa caagcagatc atcatcatca ccgacggaga
    12001 gccgaccgcg cacgtcgagc caagcggcga gatcttcttc tcgtggccgc cgacgccgca
    12061 aacctgggat gcgaccttgc gcgaggtgct gcgctgcacg cgggacggga tccgcatcaa
    12121 cacgttcatg ctcgagcggt ccacgtacct gatgaagttc gtcaacgatc tctcgaagat
    12181 caatggcggt cgtgtcttct tcgccagccc ggaccatctc ggcgaatacg tgctggtcga
    12241 ctacgtccgc aacaagcgca agtggattca gtgatcacct gatccctttt tggccccttt
    12301 cctgcgcttt cggtgcctct tgcgtgggtg tagagtgcct gttatcgatg ctcctgattt
    12361 caggcatatg agcgcctgcg gaagagcatt gttgctgacc atggaggccc gtacgtatgg
    12421 tcggtgcgct cgaacaaggc cgaacgcatt tcgatcgaca cgagtggacc gaggcgcatg
    12481 cgcactttgc gcaggctgac catgaggttc cgctcgagtc ggccgacatc gagcgacttg
    12541 cgaccgcggc atacctgatc ggccttgatg ccgagagctc caccgcctgg tcgcgcgcct
    12601 accatgagca tgtatcagcg ggtgattggg tcggggccac acggtgcgcg ttctggatgg
    12661 ggtttggcct catgaaccgc ggtgagtacg cacaggccgg tggctggctt gcccgcgcgc
    12721 gtcgccagtt ggaccagac
//
LOCUS       DATAOZ010000071         4844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_556696, whole genome shotgun sequence.
ACCESSION   DATAOZ010000071 DATAOZ010000000
VERSION     DATAOZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556696"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1336)
                     /gene="dnaN"
                     /locus_tag="VHR64_02015"
     CDS             complement(179..1336)
                     /gene="dnaN"
                     /locus_tag="VHR64_02015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX3301626.1"
                     /translation="MKVACLQEQLQRALSLVSRAVASKTALPVLGNVLLSAEDDRLQL
                     SATNLEIGISTTISASVDEPGRLTVDARLLQEFVSTLPNETVELSTEGDRTLLAVKCG
                     RDNASINGIGADEFPSTPAVSEGGFTVDVDPQVLREMIGQVEFAAATDDSRPVLAGVL
                     LRFEDNSITLAAADGFRLAVRHSPMDATVSERVSLIAPARAIRELGRILGESNEPVRL
                     AVTPNQSQLIVRVGPAGEPGGTEFYSRLIDGTFPDYRQIIPKDFGTKVELGRDALLNA
                     VRRASYFARDANDAIKFEIKSQTDAAEPGVVEVSARAAERGDSHSFVDATIDGPEMQI
                     AFNSRYLTDVLAVVRNGRIALGLNGPNQAGVVRVVGSDDYSHVIMPMVIGS"
     gene            1536..1763
                     /locus_tag="VHR64_02020"
     CDS             1536..1763
                     /locus_tag="VHR64_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301627.1"
                     /translation="MKNRTSQRTTPETDLQARKAELVRRLQDGDEQITSAKRDGADTT
                     RWENHWITLLRQYESVCREISESGDQYPAAA"
     gene            complement(1760..2452)
                     /locus_tag="VHR64_02025"
     CDS             complement(1760..2452)
                     /locus_tag="VHR64_02025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3301628.1"
                     /translation="MKRLAILGVLVLLGTVLGGCGVGNKKNDTAETTVVSAPVDADIS
                     SGCWTAEQRALPKTIEGGKQLKQWTQPPAMAIDTNKTYMATVNTNHGSFEITFFPAEA
                     PKTVNNFVCLAKSGFYDNTTFHRVVPGFVIQGGDPEGTGRGGPGYRFDDEPVKRQYNR
                     GIVAMANAGPNTNGSQYFVCLDNVNLPPAYTIFGEVTKGMDVVDKIAAVPINGSSPVE
                     PVTVSTVTISES"
     gene            complement(2449..3024)
                     /locus_tag="VHR64_02030"
     CDS             complement(2449..3024)
                     /locus_tag="VHR64_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX3301629.1"
                     /translation="MELTRENAWALLTEYVQSESLRRHCLGVEAAMRAYARKFGENEE
                     EWSFIGLIHDFDFEIHPTLDQHPQDGFPILRERGVPEWVIEVVASHADHLGEPRDTPV
                     KKTLAAVDELTGFIAACAFVRPTKAVRDVTPQAVRKKMKDKAFARAVDRQAMLDGAEL
                     LGIPFDEHVEFVIAAMAENAEELGLQGVAVE"
     gene            complement(3067..4122)
                     /gene="queA"
                     /locus_tag="VHR64_02035"
     CDS             complement(3067..4122)
                     /gene="queA"
                     /locus_tag="VHR64_02035"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX3301630.1"
                     /translation="MPPIDPTLRVSDFDYHLPQELIAQHPPADRDGGRLLLVDRAKGK
                     LTDMTVRDLPMLLRASDLVVVNNTRVIPARLSGRRPTGGAVEVLLLNRVRPGGWEALA
                     KPARKLTPGATFDLISHSGDTSVPAEVVENFGEGRILVSFDPDLDGRLEEFGAVPLPP
                     YIHEQLGDTERYQTVFSHVPGSAAAPTAGLHLSEAILAQLQDRGVERAEVTLQIGLDT
                     FRPVTVEHVADHVIHREWCVVPRETAGAIQRCREQQARVVAIGTTSARTLETWGSLSS
                     AVRANGWSGWTDIFITPGYHWTTVDAMLTNFHLPKSTLLMMISSLMGRELTEAAYRHA
                     VEQRYRFFSFGDAMFIV"
     gene            complement(4125..>4844)
                     /gene="ruvB"
                     /locus_tag="VHR64_02040"
     CDS             complement(4125..>4844)
                     /gene="ruvB"
                     /locus_tag="VHR64_02040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX3301631.1"
                     /translation="DEIHRLSRQVEEVLYPAMEDYAVDIVIGKGPSARTMRIKLPRFT
                     LVGATTRLALLTGPLRDRFGAVLRLDYYDDAAMEEIIRRSAKILAIEIDDTGTSAIAA
                     RSRGTPRVANRLLKRVRDYSQVHGSGNIHESEAREALAMLEVDDLGLDDVDRRILLSI
                     IQKFGGGPVGIDTLAAATSEETDTIMDVYEPYLIQLGFLQRTPRGRIATRLAYEHLEI
                     DPPAHLELLAGTAEQQPSLFD"
BASE COUNT          894 a         1567 c         1477 g          906 t
ORIGIN      
        1 gcaaggtcga aagcatgcgg acggcctcga gcagcgagct cttgcccgcg gcgttctgcc
       61 cgacagccac cagacccggc ggcggaacgg cgagatcgag tgcgtgatat gagcggaact
      121 cggtgagctg gatgcggcga agatgcatcc cttcagcaaa cgcgacctga agtcaggctc
      181 acgacccgat gaccatcggc atgatcacgt ggctgtagtc gtctgaccca acaacccgga
      241 cgacaccggc ctgattcggc ccgttgagcc ccagggcgat ccgtccgttg cggacgacgg
      301 ccagtacgtc ggtcagatac cgggagttga atgcgatctg catctccggt ccgtcgatcg
      361 tggcgtcgac gaaactgtgg ctatcgccgc gctccgccgc ccgggccgag acctcgacca
      421 cgcccggttc ggccgcgtcg gtctgcgatt tgatctcgaa cttgatcgcg tcgttggcgt
      481 cgcgggcgaa ataactggcc cgccggaccg cgttcaacaa ggcatcccgg ccgagctcga
      541 ctttggtgcc gaaatccttt gggatgatct gccggtagtc ggggaaggtg ccgtcgatca
      601 ggcgcgaata gaactcggtg ccgccgggct ctccggccgg accaacccgc acgatcaatt
      661 ggctctggtt cggagtcacg gcgagacgaa ccggctcgtt cgattcgccg aggatgcggc
      721 ccaattcgcg gattgcccgg gccggcgcga tcaggctgac ccgctcgctg acggtggcat
      781 ccatcgggct gtggcgaacc gccagccgga agccatcagc cgccgccagc gtgatcgagt
      841 tgtcctcgaa gcgaaggagg acacccgcca gcacgggtcg agagtcatcg gtcgcggccg
      901 caaactcgac ctgcccgatc atctcgcgaa gcacctgcgg atccacgtcg accgtgaagc
      961 caccttcgct gacggcaggc gtgctcggga actcgtcggc gccgattccg ttgatcgagg
     1021 cattgtcgcg accgcacttg acggcaagaa gcgtccgatc accctcagtg ctcaactcga
     1081 ccgtctcatt cggcagcgtg ctgacgaact cctgcaggag ccgcgcatcg actgtcagcc
     1141 ggcccggttc atcaacgctg gccgaaatgg ttgtgctgat cccgatctcg aggttcgtag
     1201 cggagagctg gagacgatca tcctcggcag agagaaggac gttgcccaaa accgggagcg
     1261 ccgtcttgga tgcgaccgct ctggacacca gactgagcgc gcgttgcagc tgctcctgca
     1321 ggcatgcgac cttcacaata aactcccacg ttccccggat acgtcagtca aaattggacc
     1381 gtgaatatag catagccgac tatcccttcg gatcatcgcc cggccgcccg ggcttcggcg
     1441 atgatcgcgt attgacacat cgtcatagaa cgtatattct gaaatcagac gcacaggtgt
     1501 tcgcatttct tcggtcaacg caggacaacg ttcagatgaa aaaccggact tcccagcgca
     1561 ctaccccgga aaccgatctc caggctcgca aggccgagct cgtccgtcgc ctccaggatg
     1621 gcgacgagca gattacgtcc gcaaagcgag acggagcaga cacgacccgg tgggaaaacc
     1681 actggatcac gctactccgt cagtacgagt cggtttgccg ggagatctcc gaatccggcg
     1741 atcaataccc cgccgctgcc taggactcgc tgatcgtcac ggtgctgacg gtgaccggct
     1801 caaccgggct ggacccattg atcggcaccg ccgcgatctt gtcgacgacg tccatcccct
     1861 tggtgacctc accgaagatc gtataggccg gcggcaggtt tacattgtcg aggcagacga
     1921 agtactggct gccgttcgtg ttcggacctg cattcgccat tgccactatg ccgcggttgt
     1981 actgccgctt gaccggctca tcgtcgaagc gatagccagg tccaccccgg ccggtgcctt
     2041 ctggatcgcc gccctggatc acgaatccgg gcacaacccg gtggaacgtg gtgttgtcgt
     2101 agaagcccga cttggccagg cagacaaagt tgttgaccgt cttcggcgct tccgccggga
     2161 agaacgtgat ctcgaacgat ccgtgattgg tgttcacggt cgccatgtag gtcttgttcg
     2221 tgtcaatcgc cattgccggc ggttgggtcc attgcttcaa ttgcttgcct ccttcaattg
     2281 tcttgggcag agcgcgctgc tccgccgtcc aacatccact tgatatgtct gcgtccaccg
     2341 gggcactgac cacggtggtt tcagccgtgt cgttcttctt gttgccgact ccgcagccgc
     2401 cgaggactgt tcccagcagg accagcacac ccaggatcgc cagccgcttc attccaccgc
     2461 cacgccttgc agacccagtt cctctgcgtt ctccgccatg gcggcaatca cgaactccac
     2521 gtgctcgtcg aacgggatgc caaggagctc cgccccgtcc agcatcgctt gccggtcgac
     2581 cgcccgcgca aacgccttgt ccttcatctt cttgcggacc gcctgcggcg tgacatcgcg
     2641 cacggctttg gtcgggcgga cgaaggcgca cgcggcaatg aacccggtca gctcgtcgac
     2701 ggcggcaagc gtctttttga ctggagtatc ccttggctcg cccaggtggt cggcgtggct
     2761 ggctaccact tcgatcaccc attcgggcac gcctcgctcg cgcaggatcg gaaatccgtc
     2821 ctgcggatgc tgatcgagcg tgggatgaat ctcgaaatcg aagtcgtgga tcaggccgat
     2881 gaagctccac tcttcctcgt tctcgccgaa ctttcgcgcg tatgcccgca tcgccgcctc
     2941 gaccccgagg caatgacgtc gcaggctctc gctctggacg tactcggtga ggagcgccca
     3001 ggcgttctct ctcgtcagtt ccatgcagat gtgttcctga tagaccgaac agccagacgc
     3061 caaatcctac acgatgaaca tcgcgtcgcc gaaagagaag aaccggtatc gctgctctac
     3121 ggcgtgccgg tatgccgctt ccgtcagctc gcgtcccatg agcgagctga tcatcatcag
     3181 gagcgtcgat ttcgggagat ggaagttggt cagcatcgcg tcgacggtgg tccagtggta
     3241 gccgggtgtg atgaagatat cggtccaacc cgaccatcca ttagcccgta ctgccgaact
     3301 gagcgatccc cacgtctcga gtgtccgggc gctggtcgtg ccgattgcga cgactcgagc
     3361 ctgctgctcc cgacaccgct ggatagcccc ggccgtctcc cggggcacca cgcaccattc
     3421 gcggtggatc acatgatcgg caacgtgttc gacagtgacc ggccgaaacg tgtccagccc
     3481 gatctggagc gtgacctcgg cccgctcgac gccccggtcc tggagttggg ccaggattgc
     3541 ttcgctcaag tggagcccgg cagtcggcgc cgcggctgaa ccaggcacgt ggctgaagac
     3601 cgtctgatat cgctcagtgt cgcccagttg ctcgtgaata tatggcggca agggcaccgc
     3661 accgaactcc tccagacgac catccagatc cgggtcgaac gaaacgagta ttcgcccttc
     3721 gccgaagttt tcgaccacct cggccggaac tgatgtatcg cccgaatgcg atataagatc
     3781 gaaggtcgcg cccggcgtca gttttcgcgc cggtttggcc agtgcttccc aaccgccagg
     3841 ccgaacgcgg ttcagcagca acacttccac cgcgccgccg gtgggccgcc gacccgaaag
     3901 gcgggccgga atcacccgcg tgttgttgac caccacgagg tcggacgcgc gcaacaacat
     3961 cggcaggtca cgcacggtca tatcggtcaa cttgcccttg gcgcgatcga caagcaggag
     4021 ccgtccccca tcgcgatcgg caggcggatg ctgcgcaatc agctcttgcg gaaggtggta
     4081 atcgaaatcg gagacgcgaa gcgtggggtc aatcggcggc acgatcagtc gaagagactc
     4141 ggctgttgct cggccgttcc ggcgaggagc tccaggtgcg cgggcgggtc gatctccagg
     4201 tgctcgtagg ccagccgggt cgcgatccgg ccgcggggag tccgctgcag gaagccgagc
     4261 tggatcaggt acggctcata gacgtccatg atcgtgtcgg tctcctcgga tgtcgctgcc
     4321 gccagggtat cgatcccgac cgggccgccg ccgaacttct ggatgatcga gagcaggatg
     4381 cggcgatcga cgtcgtccag gccgaggtcg tcgacctcca gcatggccaa tgcttcccgc
     4441 gcttccgatt catggatatt gccggagccg tgcacttgcg aataatcccg cacccgcttg
     4501 agcagccgat tggcgacgcg tggcgtgccg cgggagcgcg cggcgatcgc cgatgtgccg
     4561 gtgtcatcga tctcgatagc gagaatcttg gctgaacggc ggataatctc ctccatcgcc
     4621 gcgtcatcgt agtaatcgag tcgcagcacc gcgccgaacc gatctctgag cggtccagtc
     4681 agcagcgcga gccgcgtcgt cgcgccgact agcgtgaagc gcggcaactt gatccgcatc
     4741 gtccgggcgg agggaccctt gccgatcacg atgtcgaccg catagtcctc catcgccggg
     4801 tagagcactt cctcgacctg acgggagagc cggtggattt cgtc
//
LOCUS       DATAOZ010000072         6079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7659488, whole genome shotgun sequence.
ACCESSION   DATAOZ010000072 DATAOZ010000000
VERSION     DATAOZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7659488"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..708
                     /locus_tag="VHR64_02045"
     CDS             106..708
                     /locus_tag="VHR64_02045"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX3301632.1"
                     /translation="MQAIERREIDVVIGTQLIAKGLDLPYVTTVGITNADVGLHFPDF
                     RSGERTFQLITQMAGRAGRRTPGSRVVVQTYSPDHYVLRAAANHDVEQFYEHEIRFRE
                     EFRYPPFVRMIRYLVRGATDDDCAMEADHLVRQLGRHSRLVGAGIEVVGPAPAFIARV
                     RGEQQWHVIVKSDPDGIERMLDHLPHPPGWAVDVDPVSLL"
     gene            775..1293
                     /gene="def"
                     /locus_tag="VHR64_02050"
     CDS             775..1293
                     /gene="def"
                     /locus_tag="VHR64_02050"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX3301633.1"
                     /translation="MEIIKEGDPRLRQKAHKIKSPEPGLKKIAEDMHETMDAAPGVGL
                     AGPQVGVMRRIIVIHLAEDYAEEGSPEIRMTLLNPEIVKGYGEEVGLEGCLSIPGWVG
                     EVRRMTSITVKAMDLNGRSVRIKAEDFLARVLQHEIDHLDGILFVDRMDEDAKLMPDR
                     DEDEEEIEPVSA"
     gene            complement(1294..1950)
                     /gene="gmk"
                     /locus_tag="VHR64_02055"
     CDS             complement(1294..1950)
                     /gene="gmk"
                     /locus_tag="VHR64_02055"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX3301634.1"
                     /translation="MVTVTDEQVREGDQFISHLRGRRRPRVFIISGPSGVGKDTVIDR
                     LREIESDTHFAVTATTRPKRPTEIDGTHYYFLSQEEFANWVSDGEFLEFAAVYGQRYG
                     VPKSPVRAALQRGQDVVIKIDVQGAAAIRTLIPDSIGIFIAPESMDSLYLRLSARKTD
                     DPLVLARRFEEARHELLRAQEFDYIVFNETNGLLRAVDEIGAIIRAVRSEIRQPEIAI
                     "
     gene            2002..3786
                     /locus_tag="VHR64_02060"
     CDS             2002..3786
                     /locus_tag="VHR64_02060"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEX3301635.1"
                     /translation="MFDSLTAAALADQLSRDIENGRVQRLGLISRQAVWFEIYANRRR
                     SYLVASAENQAPAVYLTDTEPVGDRQLVTPLLLLLRKYVRGGRVVAIQQPPLERVITL
                     TFSAPRFARIGPESTELVTEEVGDDEDEELDDDDSVFTHLHCEIMGRHSNLILVDDDG
                     LIMESAKRVTPTMSRVRPIAPRRPFVPPPPRDARDPRLVTEADLVELVDRAESAHALT
                     RRLPSLFLGMSPQAAAEAVYRAQDGDEGIPAARSLAQSIRRVFEPLLIGDWHSMVYRD
                     EDDSVVAYAAQPMHHLAATYREERADTISRAIELGEGRDEAAQVGGRHSVRAGRLVDS
                     INSAIDRLSGKLAALEAEEARHGERARYREWGELIYGYLWQIQPGDTELVVEGQRIAL
                     EPDIDPREQARRYLHQYQEGKSADVHIGRARSAAELELRYLEQIRTLALQAVSIQDIE
                     DLESEWRARQPAAPTSRPPKRSTSRKRTQPTAEVKGQPIFVGRSGAENDHVTFDVAGP
                     DDTWLHARGVPGSHVIVRWNGANRDDDAVLLRAAELAAFFSQSRASGRVEVDITPRRF
                     VRKIKGAGPGMVTYRNERTVSVSPVGPD"
     gene            complement(3783..4550)
                     /gene="tatC"
                     /locus_tag="VHR64_02065"
     CDS             complement(3783..4550)
                     /gene="tatC"
                     /locus_tag="VHR64_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX3301636.1"
                     /translation="MRPDDGSDEVFEEMTLGEHLIELRTRIVRACISIGVAFIIGIIL
                     AIPMLDKIQRDSQANQGLDIRSPTDPITIYFKIALYIALGLAMPLILYQMLAFIAPGL
                     TRKEKRVVYTSLPFVALLAILGGSYGFFIAAPRALYFLSRFMRGTFSWQPDAQDVLTF
                     YLTLMLGLALAFQIPVVMFILAKIGIVTPKKMASIRKYAFIVILILSAVITPSTDPIN
                     MSIVAVPLYILYEFGIIISKIFAKTPIVHPIMPTEAD"
     gene            complement(4750..4986)
                     /gene="acpP"
                     /locus_tag="VHR64_02070"
     CDS             complement(4750..4986)
                     /gene="acpP"
                     /locus_tag="VHR64_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX3301637.1"
                     /translation="MASVYERVRSIVSERLGVDEDKVTMDAEFIGDLNADSLDLVEVI
                     MAMEQEFDTEIKDEDAENIRTVADAVKFIEEHAN"
     gene            complement(5149..5619)
                     /gene="nusB"
                     /locus_tag="VHR64_02075"
     CDS             complement(5149..5619)
                     /gene="nusB"
                     /locus_tag="VHR64_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX3301638.1"
                     /translation="MSDSSRPISRNRRQRNARRHQARILAMQILYEAEMTGHSTSEIL
                     VRTRNQGGTPDETLEYASELLTGIRARIRDIDAEIEIAAPQFPLEDIAPIDRAALQIA
                     LFEVLFGEDVPPRAAVNEAVGIAQDYGGDASARFVNGVLGNVIDRHHPEASRKR"
     gene            complement(5619..>6079)
                     /locus_tag="VHR64_02080"
     CDS             complement(5619..>6079)
                     /locus_tag="VHR64_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX3301639.1"
                     /translation="AGRDRSMTAAEPFDHHVLAEVSEDGMRGTISVAPNVLAEIIELA
                     SAGTDGLVRFVAPGRGRSRSLPIQDLEQGETKGGDWYSRSGIRVRLEHAIVDADLSIE
                     VQQGASVPALAEELKRRISEAIDRMLGLKTGTIAVHVRSIAQGESGSGGH"
BASE COUNT         1170 a         1831 c         1991 g         1087 t
ORIGIN      
        1 ggatgtcgac cctgtctcac tcctttgaaa ccgtgaaatt gcatactggg tgaggcagga
       61 tcagtttgag gacgaaagca caacgatggc ggtaatgctt cgatcttgca ggcgatcgaa
      121 cggcgcgaga tcgacgtcgt catcggcacg cagctgatcg caaagggact cgacctgcca
      181 tacgtgacca cggtcgggat cacgaacgcc gatgtcggcc tgcactttcc ggatttccgg
      241 tcgggcgagc ggacgttcca gctcatcacg cagatggccg gccgggcggg caggcggacg
      301 cccggcagtc gcgtcgtggt ccaaacctac tcgcccgatc actacgtgct gcgcgcggcc
      361 gcgaaccacg atgtcgagca gttctatgag cacgagatcc ggtttcggga ggagttccgc
      421 tacccgccgt tcgtccgcat gatccgctac ctcgtccggg gcgcgacgga cgacgattgc
      481 gcgatggagg ccgatcacct ggtccgccag cttggccgcc attcgcgatt ggtcggcgcc
      541 gggatcgagg tggtcggtcc tgccccggcc ttcatcgccc gggttcgtgg cgagcagcaa
      601 tggcatgtga tcgtgaagtc cgacccggac ggaatcgagc ggatgctgga tcatttgccc
      661 catccaccgg gttgggcggt ggatgtcgac cctgtctcac tcctttgaaa ccgtgaaatt
      721 gcatactggg tgaggcagga tcagtttgag gacgaaagca caacgatggc ggtaatggag
      781 atcatcaagg aaggcgatcc acgcctgcgg cagaaggcgc acaagatcaa gagtccggag
      841 cccgggctca agaagatcgc cgaggacatg cacgagacaa tggatgccgc gccgggcgta
      901 ggactggccg gtccgcaagt gggcgtcatg cggcggatca tcgtgattca cctcgcggag
      961 gactatgccg aagagggttc gccggagatt cgcatgacgc tcctgaaccc ggagatcgtc
     1021 aagggatatg gtgaggaggt cggcctcgag ggatgcctga gcattcccgg ctgggtcggc
     1081 gaggtgcgcc ggatgacttc gatcacggtc aaggcgatgg acctgaacgg ccggagcgtg
     1141 cgtatcaagg ccgaggattt cctcgcgcgc gtgctgcagc acgagatcga ccacctcgac
     1201 ggaatcctgt tcgtcgaccg gatggatgag gacgccaaat tgatgccgga ccgggatgag
     1261 gacgaggagg agatcgaacc cgtgagcgcc tgatcagatc gcgatctccg gctggcggat
     1321 ctcggaccgg acggcgcgga tgattgcgcc gatctcatcg accgcgcgca gcaggccgtt
     1381 cgtctcgttg aatacgatgt agtcgaattc ctgcgcccgc agcagctcgt ggcgcgcctc
     1441 ttcgaatctc cgggccaaaa ccagcggatc gtccgtcttg cgcgcgctga ggcgcaaata
     1501 gaggctatcc atggactctg gagcaatgaa gatgccgatg gagtcaggga tcagtgtgcg
     1561 aatcgcggcc gcgccctgga catcgatctt gatgaccacg tcttgcccgc gttgcagggc
     1621 cgcccggacc ggtgacttgg gcacgccgta gcgctgcccg tagaccgctg cgaactcgag
     1681 gaactcgccg tcgctgaccc agttcgcgaa ctcctcctgg ctgaggaagt agtagtgcgt
     1741 gccgtcgatc tcggtcggcc gcttgggccg cgtggtggca gtgacggcga agtgggtgtc
     1801 ggattcgatc tcgcgcaggc gatcgatcac cgtgtccttg ccaacgccgg agggcccgga
     1861 gatgataaag acacgcggcc ggcggcggcc acggaggtgc gagataaact ggtcgccttc
     1921 gcgcacctgc tcgtcggtga ccgtcaccat gatgttctcc tggcgcattg acgatcgatt
     1981 cactgggacg tctctgacct catgtttgat tcgctaactg ctgcggccct ggccgatcaa
     2041 ctctcccgcg acatcgagaa cgggcgtgtg cagcgcctcg ggctgatctc gcggcaggcg
     2101 gtctggttcg aaatctatgc caatcggcgc cggtcatacc tggtggcatc cgccgaaaac
     2161 caggcacccg cagtctacct gaccgacacc gagcctgtgg gcgaccgcca gttggtcacg
     2221 ccgctcctcc tcctgctgcg caaatatgtc aggggcggcc gggtcgtcgc gattcagcag
     2281 ccgccacttg agcgggtgat cacgctgacc ttttccgcgc cgaggttcgc gcgcatcggg
     2341 ccggagtcga ccgagctggt caccgaagaa gttggcgatg acgaggatga ggagctcgac
     2401 gacgacgact cggttttcac gcacctgcac tgcgagatca tgggccgaca cagcaacctg
     2461 atccttgtcg atgacgacgg tctgatcatg gagagcgcca agcgcgtgac tccgaccatg
     2521 agccgggtcc gaccgattgc gccccggcgt ccgttcgtac cgccaccgcc gagggatgcc
     2581 cgtgacccac ggctcgtgac tgaagctgat ctcgtggagc tagtggaccg cgctgaaagc
     2641 gctcatgcac tgaccaggcg gcttccgtcg ctattcctgg gcatgagccc gcaggccgcc
     2701 gccgaggcgg tctaccgcgc ccaggatggc gacgagggga taccggcggc acgctcgctg
     2761 gcgcaatcga tccggcgggt ctttgaaccg ctgctgattg gcgattggca ttcgatggtc
     2821 tatcgcgatg aggatgactc agtcgtcgcg tatgcggcac aaccgatgca tcatctggca
     2881 gcaacctatc gcgaggagcg tgccgatacg atctcgcgtg cgatcgaact cggcgaaggg
     2941 cgagatgaag cggcgcaggt gggtggacgt cattcagtcc gggctggacg gctagtggac
     3001 tccattaaca gtgcgattga tcgcttatcg ggcaagctgg cagcactgga ggctgaagaa
     3061 gcacggcacg gggagcgagc gcgataccgc gaatggggcg agctgatcta cgggtacttg
     3121 tggcagatcc agccgggcga taccgagttg gtcgtggagg gacagcggat cgcgctcgag
     3181 ccggacatcg accccaggga gcaggcccgg cgctatttgc atcaatacca ggagggcaag
     3241 agcgccgacg tgcatatcgg ccgcgcccgg agtgccgccg agctcgagct ccgctatctt
     3301 gagcagatac ggacattggc cctgcaggca gtttcgatcc aggacatcga ggatctcgag
     3361 tccgaatggc gagcacggca gccggcggcc ccaacctccc ggccgccaaa gcgatcgaca
     3421 agccgcaaac gaacccagcc aaccgccgag gtaaaggggc agccgatctt cgtcggccga
     3481 agcggggcag agaacgacca cgtcacgttc gatgtcgcgg gaccagacga cacgtggctt
     3541 catgcccgcg gcgtgcccgg gtcgcatgtg atcgtgcgct ggaacggggc gaaccgggac
     3601 gacgatgcag tgctgctgcg cgcggcagag ttggcggcgt tcttcagcca atcgcgcgcg
     3661 agcggacggg tagaagtgga cataacgccg cgtcgattcg tccgcaagat caagggcgcc
     3721 ggaccaggca tggtgacgta ccgcaatgag cggacggtgt cagtttcgcc agtgggcccg
     3781 gattagtcgg cttcggtcgg catgattgga tgcacgatcg gtgtcttggc gaaaatcttc
     3841 gagatgatga tgccgaattc gtacaagatg tagagcggca ccgcgacgat cgacatgttg
     3901 atcgggtccg tgctcggcgt gatgaccgcc gagaggatca ggatcacgat gaacgcgtat
     3961 tttcggatgc tcgccatctt cttcggcgtg acgattccaa tcttggcgag gatgaacatc
     4021 acgacgggta tctggaacgc cagtgcaagg cccagcatca acgtcaggta gaaggtgagc
     4081 acatcctgcg cgtcaggctg ccaggagaag gtgccccgca taaaacggct caggaagtac
     4141 agggcgcgag gagcggcgat gaagaagccg tacgagccac ccaggatcgc caggagcgcc
     4201 acgaatggca gcgaggtgta taccacccgc ttttccttgc gcgtcaggcc gggcgcaata
     4261 aaggccagca tctgatacag gatcagcggc atcgccaggc cgagcgcgat atagagcgcg
     4321 atcttgaagt agatcgtgat cgggtcggtt ggcgagcgga tgtccagacc ctgattggcc
     4381 tgggagtcgc gctggatctt gtccagcatc gggatcgcca gaatgatgcc gatgatgaag
     4441 gcgacaccaa tcgagatgca ggcgcggacg attcgggtgc ggagctcaat caagtgctcc
     4501 ccgagcgtca tttcctcgaa gacttcgtca gagccgtcgt ccggccgcat ccagtccggt
     4561 cgacttggct tgtggggcct ggggaccttg gggacccgtg gcactggcaa ttttgcgagg
     4621 cgcgccatcg acttacctga tgagcattag tggaacaaaa gggcgggcca cctagcggcc
     4681 cgcccttccg caaacgtcac tacccagtat tgcggccaat tcggccgctg acaagcgaag
     4741 cgccgcgaac tagttcgcat gctcctcgat gaacttgacc gcatcagcga cggtgcggat
     4801 attctccgca tcttcgtcct tgatctcggt gtcgaattcc tgctccatgg ccatgatgac
     4861 ctcgaccagg tcgagagagt ctgcgttgag atcgccgata aactcagcgt ccatcgtcac
     4921 cttgtcttca tcaacgccca ggcgctctga gacaatgctg cgcacacgct catataccga
     4981 cgccaacgta ctcacctcca gcgaatgcca tctctccaac cggagcatct catcaattcg
     5041 atactccggg cgcgccgaca gtctgatcga aacggaaaac cgtattcccg tcagggacgc
     5101 ccgcgccgga ttctacacga cgtcagatat ggcggccact gaaacaggtt accgcttgcg
     5161 gcttgcttcc gggtgatgcc ggtcgatcac gttgccgaga acgccgttca cgaagcgggc
     5221 gctggcgtct ccaccgtagt cctgggcgat gcccacggcc tcgttcacgg cggcgcgcgg
     5281 cggcacatcc tctccgaaga gcacttcaaa cagcgcgatc tggagcgcgg cccgatcgat
     5341 cggcgcgatg tcttcgagcg gaaactgggg cgcggcgatc tcgatctcag catcgatatc
     5401 acggattcgg gcgcggatgc cggtcaagag ttcggaagcg tactccaggg tctcgtccgg
     5461 agtgccgccc tggttccggg tgcggaccag gatctcgctg gtcgagtggc ccgtcatctc
     5521 ggcctcgtac aggatctgca tggcaaggat gcgggcctga tggcgacggg cgttgcgctg
     5581 gcggcggttc cggctgatcg ggcgagatga atcggacatc agtgacctcc ggatccactc
     5641 tcgccctgtg cgatggagcg cacatgcacc gcgatcgtgc cggtcttcag gccaagcatg
     5701 cggtcaattg cctcgctgat ccgtcgtttc agctcttcgg ccagggcagg cacgctggcg
     5761 ccctgctgaa cctcgatcga gagatcagca tcgacgatcg catgctccag ccgcacgcga
     5821 atgccactgc gcgaatacca gtcgccgccc ttcgtctccc cttgctccaa atcctggatc
     5881 ggaagcgacc gggagcggcc acggcccggc gcgacgaacc tgacgagccc gtcggtcccc
     5941 gccgacgcga gctcgatgat ctcggccagg acattcggcg cgaccgatat ggtgccgcgc
     6001 atgccgtcct ccgagacctc ggcgaggacg tggtggtcga acggttcggc ggcggtcatg
     6061 gaccgatcgc gtcctgctg
//
LOCUS       DATAOZ010000073        11304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3451861, whole genome shotgun sequence.
ACCESSION   DATAOZ010000073 DATAOZ010000000
VERSION     DATAOZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3451861"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VHR64_02085"
     CDS             <1..1615
                     /locus_tag="VHR64_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX3301640.1"
                     /translation="TVGAVVQMLAIIGIGCGVGLMLGWRPLESLFFGAVISISSSTVL
                     VKIIGDTGEIDSPHGRLTLAWSTVQDLATVILIVVLSALATDSTNLARDMVWEVTKAG
                     LFLILLIPVGLRVLPWIFDWVADLRSREVFVLSVGVAALGLAYSATFFGLSLALGAFV
                     AGIVVGESDLSHQILGDIMPLRDIFAGLFFVSVGMLVDPAFVARNLPLLLVTVLLIIL
                     VKGSISALIALVMKTRVRTAILTGALLAVSAEFSFLLARVGHDLHAESNTVFNLMLAG
                     SAVSIVLAPFVYRASQPLADAVVARMPVGEDYGVLAMPTHEPEAVLRGHAIILGFGRV
                     GRIVSDVLRKQDFRYVIVEEDPRLAKRAQAEGIPVVRGSAAVPAVLEHANPGRARVLV
                     IAIPDPVATRIVVDYVRARYPRLDVVVRTHSDEERRDLLNRGVRTVILGEWELALELT
                     RHTLQRFGVESLVTGQIINRMRTQVEPEPPEDFIDEPTRDRTSLDAALRREIEARRRP
                     EKPAEPEPAQPTPALSRPARRPRIKDVRP"
     gene            complement(1905..2543)
                     /locus_tag="VHR64_02090"
     CDS             complement(1905..2543)
                     /locus_tag="VHR64_02090"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX3301641.1"
                     /translation="MASTTTEERQVGEFQAVSLDGVGVINFVQEDATSLSIEAEPEAM
                     PLITTEVKDGTLHIQTQLLTMKAIHLKHPPVYTITSPNLNALDLRGAGRAKIGHLKAD
                     QLAITVDGAGDIRLTNQTLGACRIRIAGAGNVQAAGIATVQEIDITGTGNFSGDQFVG
                     ETAKVNIAGAGRAKVHATQDLAVDIGGVGSVTYIGDPRITPNVGGLGRVKRA"
     gene            complement(2583..3311)
                     /locus_tag="VHR64_02095"
     CDS             complement(2583..3311)
                     /locus_tag="VHR64_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301642.1"
                     /translation="MRITPVAAGAALAVAGGIAFTSVVRPWYSRWGATDAEIDRVMPL
                     DERIPDPTMSTTMAITINARPEAIWPWLVQIGDPPRAGYYSYTWIEKLVGLDVQNAGR
                     ILPEFQAVEVGQALDRNGTMIVQAVDPGHSLVLGPPPSVDYLQCTWAFALYPITERST
                     RLVTRVRARFCYGDIMRGTPVYTWPTWLLIDPGAFIMERKMLHEIKRLAESSQLTPYA
                     RAIAFDRAVARMETGATIGAAVPG"
     gene            complement(3420..3650)
                     /locus_tag="VHR64_02100"
     CDS             complement(3420..3650)
                     /locus_tag="VHR64_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301643.1"
                     /translation="MATDPDVQRELDANQQRKRVIDQNEDPNNDGTGDPVDSIDDAYQ
                     SFVEPPLMRDHPSEAEEEEQRRIQNDEQARTT"
     gene            5103..5534
                     /locus_tag="VHR64_02105"
     CDS             5103..5534
                     /locus_tag="VHR64_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301644.1"
                     /translation="MATDCASDAACGAGAICCSGQCRQADCCVDAQNPDARCTTGETC
                     VQGVCQAHSCSDLGDACSRDADCCGALYCLDGACEVERGPDDHLPGKPGPIMVTTLPR
                     TGVDDDDSGGQMAVLLGGSIASGAAGLWVAIRRYIRASSIP"
     gene            6938..7108
                     /locus_tag="VHR64_02110"
     CDS             6938..7108
                     /locus_tag="VHR64_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301645.1"
                     /translation="MPGDTNNGVTTLPNTGVGGEPDGLDEMLGITFAAGAASFLLRKK
                     VRDATDAPTDPE"
     gene            complement(7123..7461)
                     /locus_tag="VHR64_02115"
     CDS             complement(7123..7461)
                     /locus_tag="VHR64_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX3301646.1"
                     /translation="MLMTTTNALEGMPIQRYLGIVSGEAILGANLMKDLFASVRDIVG
                     GRSAAYEQELIRAKETALNEMQMNAVAMGANAIIGVDLDYETLGSGNSMLMVCVSGTA
                     VIVNYTTHAV"
     gene            complement(7525..8346)
                     /locus_tag="VHR64_02120"
     CDS             complement(7525..8346)
                     /locus_tag="VHR64_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3301647.1"
                     /translation="MTDAEARRPADTWASGDAYEPYVGRWSRMVARELVDWLDIPPGS
                     RWLDVGCGTGAISQTILDLAQPYAITAVDPSEAYTAYARRRFDGAPVEVKVGDARSIP
                     VPDGTYDAVVSGLMLNFVPAPGQPKAAAEMVRAARPGGTVAAYVWDYAGEMQFMRYFW
                     DAAVALDPENEDLDEGPRFPICQPGPLGDLFRDTGLTGVEVRALDIPTVFRDFDDYWS
                     PFLGGQGAAPAYAMALTEEHRAALRDRIRAHLPFEQDGSIHLIARAWGVRGIVAE"
     gene            8500..11292
                     /locus_tag="VHR64_02125"
     CDS             8500..11292
                     /locus_tag="VHR64_02125"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3301648.1"
                     /translation="MRSVPDQTVAGLTLLATKLYIPKWRSGLVSRPRLVEQLHRGAER
                     KLTLVTAPAGFGKTTLLAEWLAATPAGEASAGWVSLDQGDNDPGLFWAYVIAALQRVR
                     PGIGANALALLESARPPAIESVLATLINDIAEDGHDIALVLDDVHVIDAQPIHEAIAF
                     FVDHLPPNLLLVLASRSDPVLPLSRLRARGELTELRAADLRFTPDEAAAFLNEMMGLD
                     LSAGDVTALEARTEGWIAGLQLAALSMRGRSDASDFIAAFAGDDRYIVDYLVEEVLQR
                     QPETMRDFLLQTSILERLSGPLCDAVTGRDDSRMVLERLERGNLFLVPLDSRRHWYRY
                     HHLFADVLRAHLGEELPDQPAALHRRASEWYEQTGSPADALRHAFAAEDFGRAANLVE
                     LTATAMRRSRQEATLLGWLRMLPDEVFQCRPVLSAVYGGVLLSNGLFEGVEPLFLNAE
                     RWLEVVAGVGGQADAVSAGMIVVDQEEFRRLPALIAVHRAGYALAQGDLARAVRFARH
                     VLDMTPGDEHFYRGAAEAILGLASWTNGDLETAHQSYAEGMASLQRAGYITDSIGGIS
                     SLADIRVAQGRLHEAMETYRRTLQRATENSATVLRGTADLHVGMSEIYREWNDLDAAR
                     QHVLRSTELGEAAGFPQFPHRWRLALARIREVEGDLDGALDLLDEAERVYVSDFHPDV
                     RPIAAMKVRIWLLQGRLPEAWAWVRERRLSADDDLSYLREFEHITLARVLIATYQKER
                     DVSVLRQAMGLLDRLLEAAESGGRMGSVIEILLLESLAHEAGVDGSGALVPLERALAL
                     AEPEGYVRTFVDEGPPMADLLKAATRRGIAPGYAHKLLAAFGSADVRVPVMQDMLDPL
                     SERELDVLRLLGTDLSGPEIANELMVSLNTMRTHTKNIYAKLGVTSRRAAVRLAEERG
                     LVRRAGTNDSGPVPDA"
BASE COUNT         1776 a         3579 c         3846 g         2103 t
ORIGIN      
        1 gaccgtcggc gcggtcgtcc agatgctggc gatcatcggg atcggctgcg gggtcgggct
       61 catgcttggc tggcgaccgc tggaatcgct cttctttggc gcggtcatct cgatctcctc
      121 cagcacggtg ctggtcaaga tcatcggcga taccggcgag atcgattcgc cccacggccg
      181 cctcacgctc gcctggtcga cggtgcagga cctggcgacg gtgatcctga tcgtcgtgct
      241 ctcggcgctg gcgaccgaca gcacgaacct ggcccgcgac atggtctggg aggtgaccaa
      301 ggccggtctc ttcctgatcc tgctgattcc ggtcgggttg cgggtgctgc cctggatctt
      361 cgactgggtc gccgacctgc gcagtcgcga ggtcttcgtg ctctcggtcg gcgtggcggc
      421 gctcggcctg gcctacagcg cgaccttctt cgggctgtcg ctcgcgctcg gggccttcgt
      481 ggccggcatc gtcgtcggcg aatcggatct ctcccaccag atcctgggcg acatcatgcc
      541 gctgcgggat atcttcgccg gcctcttctt cgtctcggtc gggatgctgg tcgaccccgc
      601 cttcgtcgct cggaacctgc cgctgctctt ggtgacggtg ctgctgatca tcctggtcaa
      661 gggcagcatc tcggccctga tcgcgctggt gatgaagacg cgggtccgca ccgccatcct
      721 taccggcgcc ctgctggcgg tctcggccga gttctcgttc cttctggcgc gggtcgggca
      781 tgacctgcat gccgagagca acaccgtctt caacctgatg ctggccggat ccgcggtcag
      841 catcgtgctt gcgccgttcg tctaccgggc gtcgcagccg ctggcggacg cggtcgtggc
      901 gcggatgccg gtcggcgagg attacggcgt cctggcgatg ccgacccatg agccggaggc
      961 ggttctgcgc ggccatgcga tcatcctcgg cttcgggcgg gttggtcgga tcgtgagcga
     1021 tgtcctgcgc aagcaggatt tccggtacgt gatcgtcgag gaggatccgc gcctggccaa
     1081 gcgggcgcag gctgagggga tcccggtcgt gcgggggagc gcggcggtcc cggcggtgct
     1141 ggagcatgcc aatccgggcc gggcgcgggt gctggtgatc gcgatcccgg acccggtcgc
     1201 gacccggatc gtggtcgatt acgtgcgggc tcgctatccg cggctggatg tggtggtgcg
     1261 gacccacagc gatgaggagc ggcgggatct gctcaaccgc ggggtgcgga ccgtgatcct
     1321 cggcgagtgg gagctggcgc tggagctgac gcggcacacg ctgcagcggt tcggcgtcga
     1381 gagcctggtg accggccaga tcatcaaccg gatgcggacg caggtcgagc cggagccgcc
     1441 ggaagacttc atcgacgagc ccacccggga tcgcaccagc ctcgacgccg ccctccgccg
     1501 cgaaatcgaa gcccgccgcc gcccagaaaa gcccgccgag ccggaacccg cccaacccac
     1561 gcccgcgcta tcccgccccg cccgccgtcc gaggattaag gacgtgcggc catgatgcgg
     1621 gttgtatccg aaatggatgt gcaaggtgaa aagctcgagg gcactgagtt ggacgttctc
     1681 cacgcagccc cgtgctgagg ctgagtcccg aacagacgga tgatgccaac tgactttcca
     1741 gtccctttag ttgacttttt gtgaattagc ccggaccttt aactccgggc gatgccgagc
     1801 ggtccgtttc cgattcgaaa cgtgctcagt cgtgcgccga tgtcgcatag ctttccgacg
     1861 ctacgatgtg ccaccgctaa gcgtgactga ttcggccaac agcgctacgc gcgcttcact
     1921 cggcctaagc cgccgacgtt cggtgtaatc ctcgggtcgc cgatgtaggt tacggagcct
     1981 acgccgccga tgtcgaccgc caggtcctgg gtggcgtgga ctttggcgcg gccggcgccg
     2041 gcgatgttga ccttggctgt ctcgcccacg aactggtcgc ccgagaagtt gccggtgccg
     2101 gtgatgtcga tctcctgcac ggtcgcgatc ccggccgcct ggacattgcc cgccccggcg
     2161 atccggatcc ggcaggcgcc gagcgtctgg ttcgtcagcc ggatgtcgcc cgcgccatcg
     2221 acggtgatcg cgagctgatc ggccttgagg tgaccgatct tggcccgacc ggcgccgcgc
     2281 agatcgagtg cgttcaggtt cgggctggtg atggtgtaga ccggcggatg cttgagatgg
     2341 atcgccttca tggtcaggag ctgggtctgg atgtgaagcg tgccgtcctt gacctcggtc
     2401 gtgatcagcg gcatggcctc cggctcggcc tcgattgaga ggctggtggc atcctcctgg
     2461 acgaaattga tgacgccgac gccatccagc gagactgcct ggaactcgcc gacttgtcgc
     2521 tcctctgtcg tggtggatgc catgggtcgc tcctttcgcg gaggtgagcg ctgccgccgg
     2581 gatcaccccg gaacggccgc gccgatcgtt gcgccggttt ccattctagc gactgctcga
     2641 tcgaatgcga tcgcgcgggc atacggcgtc aactggctcg attcggccag ccgcttgatc
     2701 tcgtgcagca tcttccgctc catgatgaag gcgccgggat caatcagcag ccaggtcggc
     2761 caggtataca ccggcgtccc ccgcatgatg tcgccgtagc agaaccttgc ccgcacgcgc
     2821 gtgaccagcc gggtcgagcg ctcggtgatc gggtagagcg cgaaggccca ggtgcattgc
     2881 aggtagtcga ccgagggcgg cggaccgagc acgagcgaat ggccgggatc gaccgcctgg
     2941 acgatcatcg tgccgttgcg gtcgagcgcc tggccgactt cgaccgcctg gaactcgggc
     3001 agaatccggc cggcgttctg gacatcgagg ccaaccagct tctcgatcca cgtgtagctg
     3061 tagtagccgg cgcgcggtgg gtcgccgatc tggaccagcc acggccagat cgcctcgggc
     3121 cgggcgttga tcgtgatcgc catcgtcgtg ctcatggttg ggtcggggat gcgctcgtcg
     3181 agtggcatga cccggtcgat ctcggcatcg gtcgcgcccc agcggctgta ccaggggcgg
     3241 acaaccgagg tgaaggcgat cccgccggcg accgccaggg cggcgcccgc agcaacgggt
     3301 gtgatcctca tgacatgacc ctcccggccg ctccgagcga tggagcagcc ggccctaccc
     3361 tacgcgtggc ctcgccacgc cagtgatgca gttcccggga tcaatgtcgc aagatcgtgt
     3421 caggtcgttc gagcctgctc gtcgttctgg atgcggcgct gctcttcctc ctccgcctcg
     3481 ctcgggtggt cgcgcatgag cggtggctcg acgaaggact ggtacgcatc gtcgatcgag
     3541 tcgaccggat caccggtccc gtcgttattc gggtcctcgt tctggtcgat tacgcgtttg
     3601 cgctgctggt tcgcgtccaa ttcgcgctgg acatcgggat cagtcgccat cgtctgccct
     3661 ccttcgcaca tcgcgatcat gctagcgacg cggctccgca gcgcgtccgt atcgcgctac
     3721 agcatgtcga acgggcaaat gcctgtgcgc gccctgggag cgactgccag tcacggtccg
     3781 aagcctcgtc ccgcagggat gaaggattgt ggcgatgccc acaatgtgtt cccaaaatgg
     3841 ttcgctagac atattgcctt cctgtgcctg accgctagaa tgcgagtagc cgaagcgaag
     3901 acaagtgaat aggggaggca atggatggtc agcgcttcga cgacctcgcc agactgatcg
     3961 gatcaggggc gacgcgccgc cgtgtcttac aggcactcgc cggcgggttc ggctcgatcc
     4021 tgttaggaaa caacccgact cgcgcccagt gcgccaatcc agacacctgc caatccgacg
     4081 acgagtgttg cctgggatac gcctgcgatg cggcgagctc gacctgcact gaggtgctgc
     4141 cggatccctg tggatcggac ggagccttct gcgccggaga cgcggattgc tgcatcggcg
     4201 cctgtctcaa cggcctgtgc cagtgccagc cggccggcgg tccatgcacg gtcagtagcc
     4261 agtgctgcct cctcgacggc caaacgatgt gcatcaacgg cgcgtgcggc acctgctacg
     4321 acaccggcgg ggcctgctct tccgccgccg attgctgcca tggcgagtgt ttccagggcg
     4381 tctgcacgga ctgccggagc gatggctccc agtgcgcgga ctcatcccag tgttgcaacg
     4441 cggattgctt caacggcgcc tgtgtctcct gtcgctcaat gggtaccgcg tgcgattcgg
     4501 ggcacgcctg ctgcgccgga ttctgcgttc agggcgtttg cgtcacctgc aaggacaatg
     4561 gaaacgcctg cgccgctgac accgaatgct gcgccggcga ttgcttcaat gggacctgcg
     4621 tcgcgtgccg gcgggtgggc gcacagtgcg cggccggcac cacgtgctgc ggtgagtcgg
     4681 ggtgcgtcga cggggtctgc actgcctgtg gaccgggtga gacaccgtgt cccgattacg
     4741 ccaattgctg cagcggcgaa tgccgcaatg gcaccgtctg ccaacaatgt ggcggcgagc
     4801 gcgacacctg ccagacggat accgattgct gcagcggaac gtgcttccat ggccactgcg
     4861 cgacctgcgc ggacacgaat gcaacctgca cggcgagctc ggattgctgc agtggcgtct
     4921 gcgctgagac tggcgctggg ctgcgctgcg ctggtgaatg ccggggagcg ggcgtggcct
     4981 gcgttgacga ctcgagttgc tgctcgggtc attgcagggg tggcatgtgc accgcgcctc
     5041 cctgcagcag ctcggcggac tgcggctttg acaagatgtg cgtcagcggt tcctgcctgg
     5101 cggtggcaac cgactgcgcc agcgacgctg cgtgcggtgc cggggcgatc tgctgcagcg
     5161 gtcaatgccg gcaggccgac tgctgtgtcg atgcccagaa tccggacgcg cgctgcacca
     5221 ccggagagac ctgcgtgcag ggcgtttgcc aggctcattc gtgctccgac cttggcgacg
     5281 cctgttcacg cgatgccgac tgctgcggcg cgctctattg cctcgatggc gcctgcgagg
     5341 tcgagcgcgg ccccgacgac cacttgcccg gcaagccggg tccgatcatg gtgacgaccc
     5401 tgccgcgcac gggcgtcgac gatgacgact ccggcggcca aatggcggtg cttctcggcg
     5461 gctcaattgc gagcggagcc gccggactct gggtagcgat ccgccggtac attcgagcat
     5521 cgtcgatccc ataaccagac agtcggtcga tcagggacac ggggagggtt ttccgcgaat
     5581 ggacggccag cgctttgacg accttgccag agtgatgggc tccggcgcct cgcggcggcg
     5641 catgcttgcg tggcttgcgg gtagcatcgg ctcgctctgg ttgggccggg atgttgcccg
     5701 cgcccagtgc gcaaatccgt cgagttgcca gatcgatatt gactgctgcc cgggctacct
     5761 gtgcgaccac gtgaacttga cctgcatcga gttcacgacg tgcgaagtga acggcgcagc
     5821 atgcgtgacg cacgaggagt gctgtagcga gacgtgcatc gccggcgtat gcgcaacctg
     5881 cctgtctgtc ggcgcttcct gtaccgacga cgcgcaatgt tgcgtccgcg attgctctaa
     5941 cggtgtctgc ggttcctgcc tcgaagaggg ttcggcctgc acggcggaca gccagtgctg
     6001 ccatcaggac tgcttcggcg gcgtctgcct caggtgccgc ggggacggca tggcgtgcga
     6061 cagcctgttt caatgttgca cggcggattg ctttgaaggc acctgcgtct cttgccggca
     6121 aatgggtgac ccgtgcaatt tcgagtaccc atgctgcggc ccggggtatt gcatcaatga
     6181 tgtctgctca ccctgcatag agatcggctc tgcatgcagt gaatcacgcg aatgctgctc
     6241 cagcgcctgc ctcagcggtc agtgcgtcga gtgcttcccg tcgggcgagg cgtgccagtt
     6301 ccataacgaa tgttgtgaaa acgagtactg catcgacggc gtttgcgacc cgtgcctcgc
     6361 actgggtgag gcatgcgcgg accgtgtttg ctgcaccgga tcttgcacga acggcgtctg
     6421 tcaggaagcg tgccagaccg acaattctcc atgctccgat gatagccaat gctgctccgg
     6481 gagctgcctc gacggtgtat gcgcgaccgt ttgctccgag gatggaggct catgttcggc
     6541 tggtccggac tgctgcgggt attcgatggg gttgtccact tgcgccggcg ggacttgtgt
     6601 cgaggtcacg tgctcgtcgg aggactgcgt gacgctccca tgctgcccag gatcgtactg
     6661 cgttgctgag gggcaggggt gtgctgccat cgaatgcacc aacagcggcg attgccccac
     6721 aagagcggtc ggctgtcaca acggttggtg cgtcgattac gagtggtggc gttgcgcgac
     6781 gacgagcgat tgcgaccctg gcctggagtg ccgggatggg caatgcttca cgccgtgcat
     6841 cggcgcaggg agttcttgta gcgcatcggc cgattgctgt gacgcgctcg cttgtatcga
     6901 cggaacctgc cagtccgata acggcgattc aggcgacatg cccggtgaca cgaataacgg
     6961 ggtgacgacg ctgccgaata cgggtgtcgg tggcgagccg gatggcctcg atgagatgct
     7021 cgggattacg tttgcggccg gcgcggcgtc attcctgctg cgcaagaagg tgcgggatgc
     7081 gacggacgcg ccgaccgacc cggagtgagg tgaggcgctg cctcagacgg cgtgggtcgt
     7141 gtaattgacg atgacagcgg tgccgctgac gcagaccatc agcatgctgt tgccggagcc
     7201 gagcgtttcg taatcgagat cgacgccgat gatcgcgttc gcgcccatgg ccaccgcgtt
     7261 catctgcatc tcgttcaacg ccgtctcctt ggcccggatc agctcctgct cgtacgcggc
     7321 agagcgaccg ccgacgatgt cccgtacgct cgcgaagagg tccttcatca ggttcgcgcc
     7381 gagaatcgcc tcgcccgaga cgatgccgag gtagcgctgg atcggcatac cctcaagcgc
     7441 attggtggtg gtcatcaaca tcgcggaaac tcccttccct gtcccgatca attcgccgtt
     7501 cgattgtagg gggatcacgc tgggctattc ggcgacaatt ccacgcacgc cccaagcgcg
     7561 cgcgatgaga tgaatcgagc catcctgctc gaacgggagg tgggcgcgaa tgcgatcacg
     7621 aagtgcggcg cggtgctcct cggtcagcgc catcgcatag gccggcgcgg cgccctggcc
     7681 gccgaggaag ggtgaccagt agtcgtcgaa atcgcggaaa acggtcggga tgtcgagcgc
     7741 gcgcacctcg acgccggtta gccccgtgtc gcggaagagg tcgccgagtg ggcccggctg
     7801 gcagatcggg aaacgcggcc cctcgtccag atcctcgttc tccgggtcga gcgcgaccgc
     7861 cgcgtcccag aaatagcgca tgaactgcat ctcgccggcg tagtcccaga cgtacgcggc
     7921 gacggtcccg cccggtcgcg cggcccgaac catctcggct gccgctttcg gctggccggg
     7981 agccgggacg aagttgagca tcaggccgga gacgaccgca tcgtaggtac cgtccggcac
     8041 ggggatcgac cgtgcatcgc cgaccttgac ctcaactggc gcgccatcga agcggcggcg
     8101 ggcataggcg gtgtaggcct cggacgggtc gaccgccgtg atcgcgtagg gttgggccag
     8161 atcgaggatc gtctgactga tcgcaccggt cccgcagccg acgtcgagcc agcggctccc
     8221 gggcgggata tcgagccagt cgacaagctc gcgcgcgacc atccggctcc agcggccgac
     8281 atacggctcg taggcatccc cgcttgccca ggtatcggcc ggtcgccgcg cctctgcatc
     8341 ggtcatcaga actccctccc gatctcgttt cagcggttag atcagagctc gacgataatt
     8401 ggccgggtcc gcaggataaa tgcgcatatt gtgcgattaa aacgttatcg atgccggcgc
     8461 cgcgggacct ggcgggcggc gttggagcat cattcgccca tgcgtagcgt gccagatcaa
     8521 acggttgccg gactgacgct ccttgcgacc aagctgtata tccccaaatg gcgctccggt
     8581 ctggtctccc gcccgcgact ggtcgagcag ctgcaccggg gcgctgagcg caagctcacg
     8641 ctcgtcaccg cgccggcagg tttcggcaag accacgttgc tggccgagtg gctggccgcg
     8701 actccggccg gcgaggcaag cgcgggatgg gtctcgctcg atcagggcga taacgacccc
     8761 gggctcttct gggcgtacgt gatcgccgcg ttgcagcggg ttcggccggg gatcggtgcg
     8821 aatgccctcg cgctgctcga atcggctcgc ccgccggcga ttgagtcggt tctggcgacc
     8881 ctgatcaacg acatcgccga agacgggcat gacatcgcgc tcgtgctcga cgatgtccac
     8941 gtaatcgatg cccagcctat tcacgaagcg atcgcctttt tcgtcgatca cctgccgccg
     9001 aatctgctcc tggtcctcgc cagccgatcc gacccggtcc tgccgctctc tcgcctgcgg
     9061 gcgcgtggtg aattgaccga gctccgcgcc gccgatctgc gcttcacgcc ggatgaggcg
     9121 gccgcgttcc tgaacgagat gatggggctg gacctctcgg ccggtgatgt aacggccctg
     9181 gaggcacgga ccgaaggctg gattgcggga ctgcagctcg ccgcgctctc gatgcggggg
     9241 agatcggatg cgtccgactt catcgcggcc ttcgccggcg atgaccgcta catcgtggac
     9301 tacctggtcg aagaggtcct gcaacgccag cccgaaacga tgcgggattt tctgctccag
     9361 acctcgatcc tcgagcggct gagcggtccg ttgtgcgatg cggtcaccgg ccgggacgac
     9421 agccggatgg tgctggagcg cctggagcgc ggcaacctgt tcctggtccc gctggattcc
     9481 cggcggcact ggtaccgcta tcaccatctc ttcgccgacg ttctccgcgc gcacttggga
     9541 gaggagcttc ccgatcaacc ggctgcgctc caccggcgcg cgtccgagtg gtacgagcag
     9601 accggatcgc cggccgatgc gctccgccac gcgtttgcgg ccgaggattt cgggcgtgcg
     9661 gcgaacctgg tcgagctgac agcgacggca atgcgccgga gccggcagga ggcaaccttg
     9721 ctcggctggc tcaggatgct gccggacgag gtatttcagt gtcgcccggt gctcagcgct
     9781 gtctatggcg gggtcttgct gtcgaacggc ctgtttgagg gggtcgagcc cctgttcctg
     9841 aatgccgagc ggtggctgga agtggtcgcc ggcgtcggtg ggcaagcaga cgcggtatcg
     9901 gctgggatga ttgtcgtgga ccaggaggaa tttcgccgcc tccctgcctt aatcgccgtg
     9961 catcgtgccg gttacgccct ggcacaaggt gatcttgcgc gggcggtccg gtttgcacgt
    10021 cacgtgctcg acatgacccc cggggacgag catttctacc gcggagcggc cgaggcgatc
    10081 ctgggactgg catcgtggac gaacggggat ctcgagactg cgcatcagtc gtatgccgag
    10141 ggcatggcaa gcctgcagcg ggctggatac atcaccgatt ccatcggcgg catctcctcc
    10201 ctggcggata tccgggttgc gcaaggccgc ctccacgagg ctatggagac gtaccggcgc
    10261 accttgcaac gcgcaactga aaacagcgcg actgtcttgc gaggaacagc agatttgcac
    10321 gtggggatga gtgagatcta ccgcgagtgg aacgatctgg atgccgccag gcagcacgtc
    10381 ctgagaagca cggagctggg ggaggcagcc gggttccccc agttcccgca tcgctggcgc
    10441 ctagcgctgg cccggatccg ggaggtcgag ggcgatctag acggcgcact ggatctgctc
    10501 gacgaggccg agcgcgtcta tgtaagcgac ttccaccccg acgtgcgacc aatcgcggca
    10561 atgaaggtgc gcatttggct ccttcagggc aggttgcccg aggcgtgggc ctgggtacgg
    10621 gagcggaggt tgtcagctga tgacgacctc agttaccttc gcgagttcga gcacatcacc
    10681 ctcgcgcggg tgctgatcgc cacgtatcag aaagagcggg acgtttccgt cctgcgccag
    10741 gcaatgggac tcctggatcg cctcctcgag gcggccgaga gtggtggccg gatgggaagc
    10801 gtgatcgaga tcctgttgct ggaatcgctc gctcacgagg cgggggtcga tggttccggt
    10861 gcgctcgtgc cactcgagcg ggcgcttgcc ctggcggagc cggagggtta cgttcggact
    10921 ttcgtggacg aaggcccgcc catggcggac ctgctgaagg cggccaccag acgggggatc
    10981 gcgcccggct acgcccacaa actcctggct gcgtttggct cggccgacgt gagagtaccg
    11041 gtcatgcagg acatgcttga cccgttaagc gaacgcgagc tcgacgtgct ccggttactc
    11101 gggaccgatc tgtccggccc ggaaattgcc aacgagctga tggtgtcgct gaacaccatg
    11161 cggacccaca ccaagaacat ctacgccaag ctcggcgtga ccagtcgccg ggctgcggtc
    11221 cgcctggccg aggagcgtgg ccttgtgcgg agagccggca cgaacgactc cgggcccgtt
    11281 cccgacgcgt aaatccccat ccac
//
LOCUS       DATAOZ010000074         5967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9239831, whole genome shotgun sequence.
ACCESSION   DATAOZ010000074 DATAOZ010000000
VERSION     DATAOZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9239831"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VHR64_02130"
     CDS             complement(<1..223)
                     /locus_tag="VHR64_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3301649.1"
                     /translation="MVTKFASPERILVTGGAGFLGSHLCERLLKEGHEVLCVDNFYTS
                     RRSNIVHLLGLPLFEFMRHDITFPLYVEVG"
     gene            complement(382..2682)
                     /locus_tag="VHR64_02135"
     CDS             complement(382..2682)
                     /locus_tag="VHR64_02135"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX3301650.1"
                     /translation="MHRNVPARSGWEIGDARFREVGFAGLPTMADTGEIDLRGFLRAV
                     YRRKWMLIGTMVVTMGATLFWLSQATHYYDADVLIVVETRPSSIVKVDEKVPDTISDV
                     SKVNTEVAVLESRGLASRVIRDLQLDRDPEFTDATPDTDQQADDTGAETPSGGFQGEV
                     IRAVLAEALSSVRSFFVKAWAGVKGDNAPAPQQLPPEEQDSARALADSEGRDSAALLD
                     QFLKRLTIEPVEGSRLIRIGFTSTDPSKAALIANTVVDEYMKSQLETKTEGARHVAEW
                     LSDRLAELGKTVRTLEQNVQEQNTATGSNSLDIVSQRLAQINLQLIAAHAASAASQAR
                     YEQVRQVVQSGGNLDGLPEIISSPVIQALRAKHTELVGTLSEHLTIYGDNHPQILSLR
                     AEITQIEEQLNRNIANILAGLRNQMESDKIHEATVRRDLESVSEDMIHLKESDAAIAQ
                     VAQKLQVNQGLYRNLLERYTEAVALRDNQQPDARIISPAQIPLRPSFPNVPRVIALSF
                     VGSASLAVFILVIAERLRQKLDTVDSVERHVGLQILGAIPELPRLRRLASNPAGYLQR
                     QPLSEFGGAFQRLRALLNLGTTREMPRTLLVTSGSAGEGKTTVALCLGIASALSGQKV
                     LLVDCNFARPRVHRMVNVTNGRGLTDVLRGVAPLEETITRAAEYHLSILTIGRSPEGA
                     IDLLNSGRIQELLDELKSTFDVIIIDSAPVHEVSNALALGCLAEKTILVTRREWTRHR
                     DASYAAKQLQLSGADVAGVVFNRAES"
     gene            complement(2720..3433)
                     /locus_tag="VHR64_02140"
     CDS             complement(2720..3433)
                     /locus_tag="VHR64_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3301651.1"
                     /translation="MIKVVLVGMNNLFLQGLRHLLDPSQFMIAGESRDLASWYALNGG
                     GVVPNLVPDLLVADLNGCNDHDLDSLHGVRATFEDIRIVVLANELCLTDMTRLLKAGV
                     DGYLLKDLSAEALSLSLLLVMKGEKALPSTLASLLANDPRTMVANGASIRAQKHLTER
                     EEQILRCLLNAYSNKHIARALNISEGTVKVHLKSLMKKIAAGNRTQAALWARNHGMAN
                     AELMTGRSSSSSGIAQAST"
     gene            4485..>5967
                     /locus_tag="VHR64_02145"
     CDS             4485..>5967
                     /locus_tag="VHR64_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX3301652.1"
                     /translation="MAFAFKEAALSADRRALPGFDLAGEFQAGQGRLHPSGRVTMLLS
                     LVAADCLAALAAFHLGAESYLTVTGRASEAFASRPHAAVVALSLPFVYWLLDVYRVHG
                     QAPIERFPLRIKATGVLFTALYGWSYATHGFLWPLGATVPMFLLAIVLPLLGESIVRT
                     GLIRLGLWGVPAVVIGAGPIGRQVVRILQQAPELGLRPVAFFDDHYASGARPTMRVQD
                     LPVLGSIVDSAKYSRLIETAIVTTPADAQEAVDAVTMQLGYRDIIIVPDLRELPTLWV
                     RSRDLNGLIGLQVRRNLLLRRNRLLKQTTDHLIALPLALLSVPIVAVLALWIMAVSAG
                     SPFYTQLRAGKNGRPIKVWKLRTMYSDAEARLEEHLAQDADARQQWNRYFKLTDDPRI
                     LPGVGHFLRRTSLDELPQIFNVLRGEMSLVGPRPFPKYHLDQFDQSFQSLRSSVVPGI
                     TGLWQVSARSDGDLEVQQALDTYYIRNWSIWIDFYILSQTLGAV"
BASE COUNT         1034 a         1888 c         1886 g         1159 t
ORIGIN      
        1 ctccgacttc gacgtacaat ggaaaggtga tatcgtggcg catgaactcg aacagcggca
       61 gtccgagcag atggacgata ttgctgcgcc gactggtgta gaagttgtcg acgcacagca
      121 cctcgtgacc ctctttgagc aatcgttcgc atagatggga tccgagaaat ccggcgccgc
      181 ccgtcaccaa aatccgctcc ggactcgcaa atttcgtgac catcctccaa actcctgcca
      241 ggttgaccga ccggggttgc ccggttcgag aagccgtaac gctagtcggg cgcgattgct
      301 accagcaccg ctggcagcca atgttcgcga tttgagccgg agacgcccgt ctcgatccat
      361 gcaagctggg cattccgatc ctcagctttc agcgcgattg aacacgacgc cagccacgtc
      421 ggctcccgac agctggagct gcttggccgc atacgaagca tcgcggtgtc gcgtccattc
      481 gcggcgggtc accaggatcg tcttctcggc cagacaaccg agcgcaagag cattggatac
      541 ttcgtggaca ggcgccgaat cgataatgat gacatcaaag gtgctcttaa gttcgtccaa
      601 gagctcttgg atccgcccgg aattcagcag gtcgattgcg ccctcgggcg agcgaccaat
      661 ggtcagtatc gacaggtgat attcggcggc acgagtgatc gtctcctcaa gaggcgccac
      721 cccgcgaagg acgtccgtca atccccgacc attcgtgaca ttcaccatac gatgcacgcg
      781 cggccgggcg aagttgcagt cgacgagcag caccttctgg ccagagagag cgctcgcgat
      841 cccgaggcac aaagcgacgg ttgtctttcc ctcgccggcg gagcccgagg tgaccagcag
      901 ggtacgcggc atctcgcgtg tcgtgccgag gttcagaagt gcccgaagcc tttggaatgc
      961 gcctccgaac tcggagagcg gttggcgctg caagtagccg gccgggttcg acgccagccg
     1021 gcgcagacga ggcagttccg gaatcgcccc gagaatctgc agtccgacgt gccgctcgac
     1081 gctgtcgacc gtgtcgagct tctggcgaag gcgttcggcg atcaccagga tgaacaccgc
     1141 cagcgacgcc gagcctacga acgacaacgc gatcacccgc ggcacgttgg ggaatgacgg
     1201 gcgcagcgga atctgcgccg gcgatatgat tcgggcgtcc ggctgctgat tatcccgcag
     1261 cgccaccgcc tcggtgtagc gctcgagcag gttgcgataa aggccctgat tgacttgtag
     1321 cttttgcgcc acctgggcga tcgcggcatc ggattccttc agatggatca tgtcttcact
     1381 gaccgactcg aggtcacgac gtaccgtcgc ttcgtgaatc ttgtcggatt ccatctggtt
     1441 ccgcaacccc gccagaatgt tcgcaatgtt ccggttcagc tgctcctcga tctgggtaat
     1501 ctccgcccgc agcgagagaa tctgcgggtg attgtcgcca tagatggtca gatgttcaga
     1561 gagggtgccg accagttcgg tgtgcttggc ccgaagcgcc tgaatcaccg gcgagcttat
     1621 gatctcgggc agcccatcca gattgccccc gctctgcacg acttgccgca cctgctcgta
     1681 gcgcgcctga gacgctgccg acgcagcgtg agccgcgatc agctggagat tgatctgggc
     1741 cagccgctgc gagacgatgt cgaggctgtt gctgccggtc gcggtgttct gctcctggac
     1801 gttctgctcg agggtccgca ccgtcttacc cagttcggcc agccggtccg acaaccactc
     1861 cgcgacgtgg cgggcgcctt cggtcttcgt ctcgagctga cttttcatat actcatcgac
     1921 gacagtattg gcgattagtg ccgccttgct cggatcggtc gaggtgaaac cgattcggat
     1981 caaccgagag ccctcgaccg gctcgatcgt gaggcgcttc aggaactggt cgagcagggc
     2041 ggccgagtcc cggccttcgc tgtcggccag cgcacgtgcg ctgtcctgtt cctcgggcgg
     2101 gagctgctgc ggcgccgggg cgttgtcgcc cttgaccccc gcccaagcct tgacaaagaa
     2161 ggaccggacg ctgctcagcg cttcggcaag gacggcgcgg atgacctctc cctggaagcc
     2221 gccgcttggc gtttcggcgc ccgtgtcgtc ggcctgctgg tcggtgtcgg gcgtcgcatc
     2281 ggtgaattca ggatcgcggt cgagctggag atcgcggatc actcggctgg ccaggccgcg
     2341 ggactcgagt acggctacct cggtattgac cttcgagaca tcgctgatgg tatccggaac
     2401 cttttcgtcg accttcacga tgctcgaggg gcgggtctcg accacgatca ggacgtcggc
     2461 atcatagtaa tgggtcgcct gagagagcca aaacagggtc gcgcccatgg tgacaaccat
     2521 ggtcccgatc agcatccatt tgcgccggta aaccgcacga agaaatccgc gcaggtcgat
     2581 ctcgccggta tcggccatcg tcggcaggcc cgcgaatccg acctcgcgga acctggcgtc
     2641 accgatctcc cagccgctgc gagcgggaac gttccggtgc atcgcaaatc ctttccggcg
     2701 gcctcgcccg ccagcagcct caagtcgagg cctgggcaat cccgctggat gacgacgatc
     2761 gaccggtcat cagttcggca ttggccatgc catggttccg cgcccatagt gccgcttggg
     2821 tccgattgcc ggccgcaatt ttcttcatga gactcttcaa atgcaccttg acggtacctt
     2881 ccgagatatt gagagcgcgc gcgatgtgct tgttgctgta ggcgttcagc agacatctca
     2941 gaatttgttc ttcacgctcg gtgaggtgct tttgcgcgcg gatcgacgct ccattcgcga
     3001 ccatggtccg ggggtcgttg gccagcaagc tcgcgagcgt actcggcaaa gccttttctc
     3061 ccttcatgac caggaggagc gataaggaaa gcgcttctgc cgacaggtcc ttgaggaggt
     3121 agccgtcgac tcccgctttc agcaggcggg tcatgtccgt gaggcagagc tcgttcgcca
     3181 ggaccacgat ccggatgtcc tcgaaggtcg cgcggacgcc atgcaagctg tcgaggtcgt
     3241 gatcgttgca accattcaga tccgcgacca gcagatccgg aaccaggttc ggcaccacgc
     3301 cgccgccgtt cagcgcatac cagctggcga gatcgcggga ttcccccgct atcataaatt
     3361 gggacgggtc gagcaggtgt cgcaaacctt ggaggaacag gttgttcatg ccgaccaaga
     3421 cgaccttgat catgttcacg accgattctc catccgatgc gccggtgcct gcaatcccgt
     3481 agcgggtccg cggtcagtcc cgagaggcct cgagcgcgcg tgccatcggc cggcactggt
     3541 ccaaggccga gaggcagtgg cgcttcagcc gatcttgaat ggccgcgacc gaccgagata
     3601 cccctgaatt ggtattaatc ctggcggtat ttggtctagc ccacctctgt ggagcatgtg
     3661 accccaatgg agtatggcgc gactgggttc atggggtggt gtcatttatg actcgatcgg
     3721 agagggccga atggcgaaag ggcgtaggcg gcggtcttgg aaggatgggg acgagacgtt
     3781 ggcgcgcggc ccgctggcgg ctcgatgcag ccgaatccgg catattcccc gatcggcagg
     3841 cctgaaacgg cagatcgtcg ggcaaagacc gccacgggcc cactcgtcgt gccaatcggt
     3901 ccccggccca taggcggatc agattctgcc gactccagca cagggtccgc atcttacctc
     3961 gaaccgaccg ccgcttgccc tccgaaagtt atcagcgaca ggaggaatgg ccgatcgggt
     4021 gagacatccg gtacccgact ggctgttcga gctatcggga gctattccct tcttgcgcgg
     4081 tcctccacgt ctgcaacggt actgggggtc attgctccat agtagcgttc ggatctcccg
     4141 gggcagctac gcatgcattc tctttgagcg cgcatgcatt gtctcaagga gggttcgaat
     4201 gacgcggcga ttaggcgggt tcgataccgt tgcctcgatc ggttggcgcc tgatcggccc
     4261 agctcaggct ggacactgcg gttctcgagc atccggtagc gggatcggcg cggaccaagg
     4321 ctccggggct cgcagttgcc tggtgccggc ctggcgcggc gcgcgatcgg agcacgggat
     4381 ggcagcgccg tacctgcgag actcggcatt cgacacaact ttcccccggg tgcaagccga
     4441 cgccggcgcg gccatgcatc ggacttggtt gacggtgcgt tgagatggcg tttgcgttta
     4501 aagaggccgc gctctctgcg gatcgtcgcg ccctcccggg cttcgatctt gcgggcgagt
     4561 tccaagccgg gcaaggccgt ctgcatccgt ccggccgcgt gacgatgctg ctttcgctgg
     4621 tggcggcgga ctgtctagcc gcgctggcgg cgttccatct cggagccgag agctacctga
     4681 cggtcaccgg ccgggcttcc gaggcgttcg cgagtcgtcc gcatgccgcc gtcgtggcgc
     4741 tctcgctgcc cttcgtatac tggctcctgg acgtctatcg tgtgcacggc caggcgccga
     4801 tcgagcggtt tcccttgcgc atcaaggcga cgggcgtgct gttcacggcc ctgtacggct
     4861 ggtcctacgc cacgcatggg tttctctggc cgcttggtgc cacggtgccg atgttcctgc
     4921 ttgcgatcgt gctgccgctg ctgggggaga gcatcgttcg gaccgggctc atccggctcg
     4981 gcctctgggg cgttccggcc gtagtgatcg gggccgggcc gatcgggcgg caggtggtcc
     5041 gaatcctgca gcaggccccg gaactgggcc tgcggccggt ggccttcttc gacgaccatt
     5101 acgcgagcgg cgcaaggccg acgatgaggg tgcaagacct gccggtcctc ggctcgatcg
     5161 tcgacagcgc caagtacagc cgcctgatcg agaccgcgat cgtcactacg cctgcggatg
     5221 cccaggaggc ggtcgacgcc gtcacgatgc agctcggcta tcgcgacatc atcatcgtgc
     5281 ccgaccttcg cgagctgccg acgctatggg tccgaagccg tgatctcaac ggcctgatcg
     5341 gcctgcaggt gcggcgcaat ctgctgctca ggcgcaaccg cctgctcaaa cagacgaccg
     5401 accacctgat tgcgctgccg cttgctcttc ttagcgtgcc gatcgtggcg gttctcgcgc
     5461 tctggatcat ggcggtcagc gccggatcac cattctacac ccagctgcgg gccggcaaga
     5521 acgggcgtcc gatcaaggtc tggaagctca ggaccatgta ctccgatgcg gaagcccgcc
     5581 tggaggagca cttggcccag gatgctgacg ctcgccagca gtggaaccgg tatttcaagc
     5641 tcaccgacga cccgcgcatc ctgccgggcg tcggccactt cctgcgccgc accagcctcg
     5701 acgagctgcc gcagatcttc aatgtcctcc gcggcgagat gagccttgta gggccacggc
     5761 cattcccgaa gtaccacctg gaccagttcg accagagctt ccagtcgctc cgctcgagcg
     5821 tcgtgcccgg gatcacgggg ctctggcagg tgtccgcgcg cagcgatggc gatctcgagg
     5881 tgcagcaggc gctcgacacc tactacatcc ggaactggtc gatctggatc gacttctata
     5941 tcctgtcgca gacgctcggc gcagtcc
//
LOCUS       DATAOZ010000075         8806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8188363, whole genome shotgun sequence.
ACCESSION   DATAOZ010000075 DATAOZ010000000
VERSION     DATAOZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8188363"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VHR64_02150"
     CDS             complement(<1..819)
                     /locus_tag="VHR64_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX3301653.1"
                     /translation="MPNQLNGDPGEVVISASIAYDYLMSFGGSLADHILPDKTHVISV
                     SFLVDSLRRYRGGVAGNICYNLSLLGVRPTLVGAVGSDFGPYRAEIEALGIDASQVLQ
                     RDDHLTASAFMMADMKSNQIASFYPGPAEFAQEIDAKALGDRVPYALLGATGPDTMRK
                     HTRELGAASCKLIYDPAFQIILLSGEDLLEGIDAAWCVVGNDYEFAMMERKTGLTVDA
                     IAERCELVVVTYGEKGSELRMNGNCARVPAAPTRGVKDPTGAGDAYRAGLIKGLL"
     gene            complement(941..1456)
                     /locus_tag="VHR64_02155"
     CDS             complement(941..1456)
                     /locus_tag="VHR64_02155"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEX3301654.1"
                     /translation="MDEGSSEIEAIDVQFIRPEALGEPGQRRFRLLVVINGHTVIAWM
                     EKEQVGRLGELLLDVLERLPSVSELPGRPEPAILPDENTADQFRAGRMELSYDEEDDR
                     LIVVIHDIEADEGTRPALSFRLTRAQTRKFSEEADALLSAGRPTCPMCGRPMGPGPHV
                     CEKQNGHFHAD"
     gene            1550..2275
                     /locus_tag="VHR64_02160"
     CDS             1550..2275
                     /locus_tag="VHR64_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3301655.1"
                     /translation="MASTDIIETSQHAQTEPAAAIPTLEQVRDDEEVQAYIRQANKNL
                     GAAGYTEHGFRHVGLCANIARNVLRLLGYSQRDQELAAIAAYLHDIGNVISRSGHSVS
                     GALLAKPILERLGMSWSEIAIVLGAIGAHDTDEGMTEPISPISAALILADKTDVHRSR
                     VRNPDPATFDQHDRVNYAATASFLRVNADEKTITLELAIDVSIAPVMHYFEIFLPRML
                     MCRRAAEHLGCAFHITMNDVSVL"
     gene            2288..3397
                     /locus_tag="VHR64_02165"
     CDS             2288..3397
                     /locus_tag="VHR64_02165"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX3301656.1"
                     /translation="MFDYRWRYFDFYMFATTAILMGFGVVAVWSAAGAEAPTLLNPGV
                     KQAMFGAIGVVLMLVIANTDYRFIGALAWPAYFGSLLVLMAVKVFGVEIAGSRRWFDF
                     GFATIQPSEFGKITTAVTLAWFISSRGERMKEFGNFVVSMMIVGLPAALVFLEPDLGS
                     TMVYLSTWLGMMIVARTRVIYLVGLFLAAIPTVYIAWSRVFEDYQRERFDSFLNPEKH
                     MTGEAFNLIQSRIAVGSSGWFGHGIHGGSQSTEDFLKVKTTDFIFSHVSSMFGFVGMV
                     ALLLLFGVLLWRFIRVIEVSKDSFGQCLAAAVFMMVFFQTFVNIGMNIGILPVTGITL
                     PFISQGLSSVWMLLIAEGLLQSILMRHRKLAFQPD"
     gene            complement(3385..4218)
                     /gene="rsmI"
                     /locus_tag="VHR64_02170"
     CDS             complement(3385..4218)
                     /gene="rsmI"
                     /locus_tag="VHR64_02170"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX3301657.1"
                     /translation="MGTLFVVATPIGNLSDLSDRAREVLWHCPTIVAEDTRRTNQLLI
                     GIGGSARLISLTEHNLSRRIDPILAALGDGDVALVTDAGTPAISDPGFQIVEAALRAG
                     YTVRPIPGPSAVIAALSVSGLPATPFTFLGYAPRTAGATERWVDQWRRSGNTVVFFDA
                     PGRVVRTIAAINDVDGMIDLVVCRELTKLFEQVVRGTAAEIVTCFADGTIPQKGEFVI
                     VARAAGEAQDIDVDGLLRDHLAAGERPNQAAKSVAAATGLSKSELYKRALELRSEPQS
                     G"
     gene            4335..6146
                     /gene="dnaX"
                     /locus_tag="VHR64_02175"
     CDS             4335..6146
                     /gene="dnaX"
                     /locus_tag="VHR64_02175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX3301658.1"
                     /translation="MPASAPLPLNRKYRPQSFDSDELVGQEHIVRTLKNAIRSNRIAP
                     AYLFCGPRGTGKTSTARILAKAANCLDPDPDKRPCNQCAACVAINTGGTTDVIEIDAA
                     SNRGIDDIRDLRDRVNYAPTQLKTKFYIIDEAHQITGAAANAFLKTLEEPPAHTRFIL
                     ATTDPEELLPTIVSRCQRFDFRRHTPEIIARRIRALAEREGIKLADDALSLIADLAHG
                     SLRDPIGLLDQLSNYQAASGSELSADDVRSLVGISHSEVAVRIFVAISEGDAKAALQA
                     INEAVIAGQDPRQLNRQVVSLIRDGFYIASGARSSTDEPQIAEASERLGLPGLLHVAT
                     SFADTDSGIRSAVIPQLPLEMAVLDAIIGPAQSMPSVENADRPTAMPAQTTARTQSRP
                     VEPAPSRLSMREPEPVEIPDTPAQSSPGLAALVRDRDRSAPRPAREATQSSTKSKSTD
                     VVANDNGASTISVGDLSIEMLVDIWPRIRADVKSLDRRTEALLMEIDPVDITADEIVL
                     AAAYEFHRDKLNEDDRRALVESVIGRRVGRTIRVACVLRSEYTPAGSRKARTTAPAAP
                     PPAEAVNQSAVEPDAESPDEMMLGAVVRIFDGEIIED"
     gene            complement(6164..7453)
                     /locus_tag="VHR64_02180"
     CDS             complement(6164..7453)
                     /locus_tag="VHR64_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301659.1"
                     /translation="MLSLLIVLLALAACGGGEDDSNRTFAPETGGGTVEQTPTVGPTL
                     TAPPIDRQPGEGVAIATASDTLFKETGPASAIASGIDQVTIVNLDNGKAHDLPVRRSS
                     AVLAVAAPDGSQCLVIDRSGMTVEVRLYGREGKELARWSPKSAATPVAATPEPTAAPG
                     PKIIQTGDHIAWKKDGSGAIVSIGGVGIFLADNKLKMTEVKAAQSLIVTAVAWSPSEE
                     SIAVGAWDGAHQAAVLTTVSVQSLQSPGTSVLVLPEGDGRYVRSLAWGSEKVGLVFAL
                     RAASSNFSLPNDLYYLPRFGQPMQLLATAGIAAPAAVVDDVAIARNGTTVAFTILIPG
                     QVGLRFHSVWVTDALSPAPVQADTSGLRRVSELEWGTDGLAVSGTRRAQENGAAYQIA
                     VVERLDAEKSDQINADRSPATPIASPVASPVAATPKD"
     gene            7635..8630
                     /locus_tag="VHR64_02185"
     CDS             7635..8630
                     /locus_tag="VHR64_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX3301660.1"
                     /translation="MFERLSAEPIYNTRAVVRRTGVPADTFRAWERRYGVPSPLRTAG
                     NQRLYSERDIRIITWLREQTAVGLTISQAILLMRSSKIPAPTASVPTTAPLDPEVEEV
                     LSGKRSESHDALTTQRRRIVDAFSRLDGQAAEHILQETYAWAELEVVLADLLHGAVSE
                     IVRSPEYLSPHSPVSHFARAFVQRKFASLYNLSNPNDGRGPLLAASVAGESRDLTLLL
                     SAIFLSRAGYRMVFLGPDLGFDALIEAIRIVRPVAVCLAAADENTVATLKDWIPKLAN
                     VSDGNGFEYGKIPICYTGRIFVDQPEIREQITGHFLGPSGQNAVEAVEVALGGRR"
BASE COUNT         1676 a         2861 c         2619 g         1650 t
ORIGIN      
        1 cagcagcccc ttgatcaggc cggcccggta ggcatcgcct gctccggtcg ggtccttgac
       61 gcctcgcgtc ggggctgccg ggactcgcgc acagttcccg ttcatccgca gctcggaacc
      121 cttctctccg tacgtcacca cgaccagctc gcagcgctcg gcgatggcgt cgacagtcag
      181 accggtcttt cgctccatca tcgcaaactc gtagtcgtta cccaccacgc accaggccgc
      241 atctatcccc tctagcagat cttcgccgct gagcaggatg atctggaaag caggatcgta
      301 gatcagcttg cacgaggccg caccgagctc gcgggtgtgc ttccgcatcg tatctggacc
      361 cgtcgcgccg agcagcgcgt acggcacccg atccccaagc gctttggcgt cgatctcctg
      421 ggcaaactca gccggcccgg gatagaacga cgcgatctgg ttgctcttca tgtcggccat
      481 catgaaggca gaggcagtca ggtgatcgtc acgctgcaaa acctgcgacg catcgatacc
      541 gagtgcttcg atctccgccc ggtacggacc aaaatccgag ccgaccgctc cgaccagggt
      601 cggccgaacg ccgagcagcg acaggttgta gcagatattg ccggccacgc ctccccgata
      661 ccgccgcagg gagtccacca ggaaactcac ggagatgacg tgcgtcttat ccgggagaat
      721 gtgatccgca agggaccctc cgaacgacat caaatagtcg taggcgatgg acgcgctgat
      781 tactacctcg ccggggtcac cgttgagctg gttgggcacg gggcaaaagc accttgtggc
      841 taagtggaac aggcgaaacg cccgccaggt tacgcgggcg ccgacgggca acgatagcac
      901 gaagcgccat cgccgggcca gatttcaaca tcgccgatcg ctagtcagcg tggaaatggc
      961 cgttctgctt ctcgcaaaca tgcggtcccg gccccatcgg tcggccgcac atggggcagg
     1021 ttgggcgacc ggcggacaag agcgcgtccg cctcttcgct gaacttccgg gtctgcgccc
     1081 gggtcaaccg gaacgaaagt gccggtcgcg tcccctcatc ggcctcgatg tcatggataa
     1141 cgacgatgag gcgatcgtcc tcttcatcgt agctgagctc catccgcccg gctcgaaact
     1201 gatccgcggt attctcgtcc gggagaatcg ccggctccgg gcgaccgggg agctcggaca
     1261 cgctgggcaa gcgctccagc acatcaagca gcaattcgcc gagccgcccg acttgctctt
     1321 tttccatcca ggcgatgacg gtgtggccat ttatcacgac gagcagccgg aaacgtcgct
     1381 ggccgggttc acccaaagcc tccggacgta taaactggac atcgatcgct tcgatctcgc
     1441 tggaaccttc gtccacgtac cttctcgtct ccaacgcctg tcagcagcag tgtcaatact
     1501 cggtgaaact gcaccgatcg ggcaaatggt agaccgaacg cgagtagtta tggcatctac
     1561 cgacatcatc gaaacgtctc aacacgcaca gactgagccc gcagcggcga tcccgaccct
     1621 ggagcaggtt cgcgatgacg aggaggtcca ggcctacatt cgccaggcca acaagaacct
     1681 cggcgcagcc ggctacaccg agcacggatt ccgacacgtc ggcctctgcg ccaacatcgc
     1741 ccgcaatgtc ttgcgcctgc tcggttacag ccagcgggac caggaattgg ccgcgattgc
     1801 agcgtacctg cacgacatcg gcaacgtgat cagccgctcg ggccactcgg tcagcggcgc
     1861 cttgctggcg aagccgatcc tcgagcggct gggcatgtca tggagcgaga tcgccatcgt
     1921 gctcggcgcg atcggcgcgc acgataccga cgagggcatg accgaaccga tcagcccgat
     1981 ttcagcagcg ctgatcctgg ccgacaagac cgacgtgcac cggtcccgtg tccgtaaccc
     2041 ggatccggcg acatttgatc aacacgaccg cgtgaattac gctgctaccg catcgtttct
     2101 tcgcgtcaat gccgacgaga agacgataac gctcgagctc gcgattgatg tcagtatcgc
     2161 cccggtgatg cattacttcg agattttcct cccgcgcatg ttgatgtgcc ggagggcggc
     2221 agagcacctc ggctgcgcgt tccacatcac gatgaacgac gtgtcggtgc tgtaggcagc
     2281 gagcttcatg ttcgattacc gctggcgcta cttcgatttc tacatgttcg cgacgaccgc
     2341 aatcctgatg ggattcgggg tcgttgccgt ctggagtgct gccggcgcgg aagccccgac
     2401 cctgctgaat cccggcgtca aacaagccat gttcggcgcg atcggcgtgg tcctgatgct
     2461 ggtcatcgcc aataccgatt accgcttcat cggcgcgctg gcctggccgg cctatttcgg
     2521 atcgttgctg gtgctgatgg cagtcaaggt gttcggcgtt gagatcgccg gatcgcgccg
     2581 atggttcgac tttggcttcg cgacgatcca accatctgag ttcggcaaga tcacgaccgc
     2641 agtcacgctg gcctggttca tctcatcgcg cggcgagcgg atgaaagaat tcggcaactt
     2701 cgtcgtctcg atgatgatcg tcgggttgcc agccgcactc gtcttccttg agcccgatct
     2761 cggctcgacg atggtctatc tctcgacctg gctgggcatg atgatcgttg cccgcacccg
     2821 cgtcatctat ctcgtcggtc tcttcctggc ggcgatcccg acggtctaca tcgcctggtc
     2881 gcgcgtcttc gaggattacc agcgcgagcg ctttgactcg ttcctgaacc ccgagaagca
     2941 catgaccggc gaggcattca accttatcca gtcccgcatc gcagtcgggt caagtggatg
     3001 gttcggccac ggtatccatg gcggctcgca aagtactgag gatttcctga aggtcaagac
     3061 gaccgacttc atcttctcgc acgtaagcag catgttcggt ttcgtcggca tggtcgcgtt
     3121 gctgctgctt tttggcgtgc tgctctggcg cttcatccgc gtgatcgagg tctcgaagga
     3181 ctcattcggg cagtgccttg cggcggccgt cttcatgatg gtcttctttc agacctttgt
     3241 gaacattggt atgaacatcg gcatcctgcc ggtaaccggc attacgctgc cattcatcag
     3301 ccagggcctc tcatcggtct ggatgctgct gattgccgag ggattgctcc agagcattct
     3361 gatgcgccat cggaagctcg cgtttcagcc cgactgaggc tctgacctga gttccagcgc
     3421 acgcttgtaa agctccgact tcgaaagccc ggttgccgct gccacagatt tcgcggcctg
     3481 gttcggccgc tcgcctgcgg ccaggtggtc ccgcagaagt ccatcgacat caatgtcctg
     3541 cgcttccccg gcggccctgg cgacaatgac gaattcgcct ttctgcggga ttgtcccatc
     3601 cgcgaagcag gtcacgatct cagcagcagt accgcgcacg acctgctcga agagtttcgt
     3661 cagctcccgg cagaccacca ggtcaatcat tccatcgacg tcgttgattg cagcgatcgt
     3721 gcgcacgact cgaccgggcg cgtcgaagaa gacgacggta tttccggagc gacgccattg
     3781 atcgacccag cgctcagttg cacccgccgt gcgcggtgca tagccaagga aggtaaatgg
     3841 ggtcgcgggc aagccagaca ccgagagcgc ggcaatcact gccgacggac cggggatcgg
     3901 tcggaccgtg tacccagccc gaagtgcggc ctcgacgatc tgaaatcccg gatcgctgat
     3961 tgccggcgtg cctgcgtcgg tgaccagggc cacgtctccg tcgccaagcg cggcgagaat
     4021 cggatcaatg cggcgcgaca ggttgtgctc agttaggctt atcagacgcg ccgagccgcc
     4081 aattccgatc aggagctgat tcgtccgccg agtgtcctcg gccacgatcg tcgggcaatg
     4141 ccatagcacc tcacgcgccc ggtctgaaag gtcagacaga ttgccaattg gcgtcgcgac
     4201 gacgaagagc gtgcccactg tgatatgcct cgcaccgctc tcgcggcgta tactcgaacg
     4261 tctgatcccg acccaatgaa ggttccgatt tcgtgagccc agccaggaag aaacacaacc
     4321 aagacgaggc gccggtgcct gcctccgccc cacttccact caaccggaag tatcgccccc
     4381 agtcgttcga ttcggacgaa ttggtcggcc aggagcatat cgtccgcacg ctcaagaacg
     4441 cgattcgctc gaaccggatt gcacccgcgt acctgttttg cgggcctcgc ggcaccggca
     4501 agacttccac agcccggatt ttggccaagg cagcaaactg cctcgatccc gatccggaca
     4561 agcggccatg caaccaatgt gccgcctgcg tcgcgatcaa caccggcggg acaaccgatg
     4621 tgatcgagat cgatgcggcc agcaatcgcg gcatcgatga catccgcgac ctgcgcgacc
     4681 gggtcaatta cgccccgaca cagctcaaga ccaagttcta catcatcgac gaggcacacc
     4741 agattaccgg agcggccgct aacgccttcc tcaagacgct cgaagagccg cccgcccata
     4801 cacgcttcat cctggccacg accgatcccg aggagttgct cccaacgatc gtctcccgct
     4861 gccagcggtt cgacttccgt cgccacacgc cggaaatcat cgccaggcgg atccgcgcgc
     4921 tggccgagcg ggagggtatc aagctcgcgg acgatgcgct ctcgctgatc gccgacctag
     4981 cccacggaag cttgcgagat ccgatcgggc tgctcgacca gctctcgaac taccaggctg
     5041 ccagcggatc agagctgagc gcggacgatg tgcgctcgtt ggtcggcatc agccattcag
     5101 aagtcgcggt gcgcatattc gtcgcgatca gcgaaggcga tgcgaaggcg gcactccagg
     5161 caatcaatga ggcggtaatc gccggtcagg atccgcggca actcaatcgc caggtcgtgt
     5221 cgctaatccg ggatgggttc tacatcgcca gcggagccag atcaagcact gacgagccgc
     5281 agatcgcgga ggccagcgag cggctcgggc tcccgggact attgcatgtc gcgacctcgt
     5341 tcgccgacac ggacagcggc atccgctcgg ccgtgatccc gcagctcccg ctcgagatgg
     5401 ccgtcctcga cgccatcatc ggcccggctc aatccatgcc cagcgtggaa aacgcagacc
     5461 ggccaactgc catgccggcc cagacgacgg cgcgaacgca atcgcgtcca gtcgagccag
     5521 cgccgtcccg tctttcgatg cgagaacctg agcccgtcga aattcccgat actcctgcgc
     5581 aatcttcgcc cggactcgcc gcgcttgttc gcgatcggga tcgcagcgct ccgcgcccgg
     5641 cgcgcgaggc gacccaaagc agcacaaaat ccaaatcaac cgacgttgtt gcgaacgaca
     5701 acggcgcttc gaccatctca gtcggcgatc tttcgatcga gatgctggtt gatatctggc
     5761 cccgtatccg tgccgacgtc aagtcgttgg accgccggac ggaagccttg cttatggaga
     5821 tcgaccccgt cgacatcacg gccgacgaga tcgtcctggc cgcagcatat gagttccaca
     5881 gggacaaact caatgaggac gaccgccgcg cgctggtcga gtcggtcatt ggtcgacggg
     5941 tcggccggac gatccgagtc gcgtgcgtgc ttcgctccga atacaccccc gccggttccc
     6001 gaaaggcgcg gactactgcg cccgccgcgc caccgcccgc cgaagcggtc aaccagtctg
     6061 ccgtagaacc cgatgcggag agcccggatg agatgatgct cggtgcggta gtccgcatct
     6121 tcgacggaga gatcatcgag gactgagttg gggctgttcg acctcagtct ttcggggtcg
     6181 cggctaccgg gctggcgacc ggcgaggcaa tcggagtcgc cggacttcga tccgcgttga
     6241 tctgatccga cttctccgca tcaagtcgct cgacgaccgc aatctgatat gcagcgccat
     6301 tttcctgcgc acgccgtgtt cccgagacgg ccagcccgtc cgttccccat tcgagctcgc
     6361 tgactcggcg aagacccgag gtgtccgcct gcacgggcgc aggagatagc gcatcggtca
     6421 cccagacgct gtggaaccgg agaccgacct gcccgggaat gagaatcgtg aaggcgaccg
     6481 tcgtgccatt ccgcgcgatt gcgacgtcat cgacgaccgc ggccggggcc gcaattccgg
     6541 ccgttgccag cagctgcatc ggttgtccga agcgcggcag gtaatagagg tcgttcggta
     6601 gtgaaaagtt ggagctggca gcgcgcagag cgaagacaag ccccactttc tccgatcccc
     6661 aggcgagcga ccgcacatat cgaccgtcgc cctcgggcag gacaagcacg gatgtccccg
     6721 ggctctgcag cgactgtacg cttaccgtcg tcaagacggc cgcctggtgc gcgccatccc
     6781 acgcgccaac cgcaatcgac tcctcggagg gcgaccatgc cacggccgtc acaatcagcg
     6841 actgtgcggc cttgacttcg gtcatcttca acttgttgtc tgccaggaaa attccaacac
     6901 caccgataga gacgatcgca cctgatccgt ccttcttcca ggcgatatgg tctccggtct
     6961 ggataatctt cgggccaggc gcggctgttg gttccggggt agcagctacc ggggttgcag
     7021 cgctctttgg cgaccagcgt gcgagctcct tgccctcccg accatagagg cgcacctcca
     7081 ctgtcatccc ggagcggtcg atcaccaggc actgggatcc gtctggagcg gcgacggcaa
     7141 gcactgccga cgaccgcctg accggcaaat catgagcttt gccgttgtcc aggtttacga
     7201 tcgttacctg gtcgattccg gacgctatgg ccgacgccgg acccgtctcc ttgaacaagg
     7261 tatctgatgc ggtcgcgatg gcgacgcctt cgccgggctg ccggtcgatc ggtggagcag
     7321 tgagcgtcgg acctactgtg ggcgtttgct cgaccgttcc tcccccagtt tcgggagcga
     7381 acgtacgatt cgaatcgtcc tccccgcctc cgcacgcggc aagcgccagc agcacgatca
     7441 acagcgaaag cattgcgggt aagcgaccag acctcatcga acaaaacctg tgagcggctt
     7501 gcacaccgat tgcccgggat attcactcat ggtaagtgca ggagtagcgc aatcggtgtg
     7561 caaaacattg acgtcccacc aacatagccg gataatctct caatgtacgc gccgcctgcg
     7621 ttggggttcc atatatgttt gagcggttga gtgccgagcc aatttataac actcgggcag
     7681 tcgtccgccg cacaggggtc ccagcagata cgtttcgggc atgggagcgc cggtatggcg
     7741 tgccgtcccc acttcgcacg gccggcaacc agcggctcta ttccgaacgc gacatccgta
     7801 tcatcacatg gctgcgggaa cagactgcgg ttggcctgac gattagccag gccattctct
     7861 tgatgcggtc cagcaagatt ccggcgccaa ccgcgtcggt gccgactacc gcaccgctcg
     7921 acccggaagt cgaggaggta ctgagcggga agcgcagcga gtcgcatgat gcgctgacca
     7981 cccagcgccg gcgaattgtc gacgcgttca gccggctcga tggtcaggcg gctgagcaca
     8041 tcctgcagga gacctacgcc tgggccgagc tcgaagtggt gcttgcggac ctgctgcatg
     8101 gtgcggtatc cgagattgtc cgctcgccgg aatacctctc gccgcattcg ccggtgtcgc
     8161 atttcgcgcg cgccttcgta cagcgcaagt tcgcttccct gtacaacctc tcgaacccga
     8221 acgatgggcg aggcccgctc ctggccgcgt cagtcgcagg cgaatcgcga gatctgacgt
     8281 tgcttctgtc cgcgatcttc ctgtcacggg ccggctaccg gatggtcttc ctcgggccgg
     8341 atcttggctt cgacgcactg atcgaggcta tccggatcgt gcggccggtt gcggtctgtc
     8401 tcgcggcggc cgacgagaat acggtggcga cgctcaagga ctggattccg aagttggcga
     8461 acgtgtcaga cggcaacgga ttcgaatacg gcaagatccc gatctgctac accggccgga
     8521 tcttcgtcga ccagccggag atacgagagc agatcacggg ccatttcctc ggcccgtccg
     8581 gccagaatgc cgtcgaggcg gttgaggtgg cgctgggcgg acgccgctaa caggtgcccg
     8641 aatcggccgt gatagactca cggacgattg gtcgttcgaa ccattggatt ccggcggata
     8701 tcaatgaaac tcacccttga gcaggtcagg aatgtggccg ctctcgcccg tcttgggctt
     8761 tcagatgagg aaaccgagcg catgcgcgat cagctttcct cgatcc
//
LOCUS       DATAOZ010000076         7639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7924720, whole genome shotgun sequence.
ACCESSION   DATAOZ010000076 DATAOZ010000000
VERSION     DATAOZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7924720"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VHR64_02190"
     CDS             <1..180
                     /locus_tag="VHR64_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301661.1"
                     /translation="LPLLSEDQRRVLELRLAGLSSGEIEIVLGKSRSWVGVIQHRAVI
                     RLRALMADRAEPKER"
     gene            181..1542
                     /locus_tag="VHR64_02195"
     CDS             181..1542
                     /locus_tag="VHR64_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX3301662.1"
                     /translation="MRWLRNWNHRDQTDALNEFWDDVVQRSPATPLTRDPVPTELGAI
                     VRQLHAEQDARQQRPAYADRQLAVLLSHHREAQSMNTLNTYPLRTSDVRSVPSASVAG
                     RLLGHNQRRSWIPAISAGAAIAILLAVIVALAIFANLFGNHDNHPVILAPSTPTSQAS
                     PSPTVEASLVWTYTGEGEDKLDNSTTGTIAPDGSIWVVDAANARIQILDPDGSLQRMY
                     GVAGTRDGQFMFDMGPGYAGEVAVNPDGSFYVAEAGNARVQKFTDNGKFAVSWSGATT
                     PIQTFSLLIGVSIDSDGNVWTADESANMISKFTPDGRFLLAIDGIDTAQHPEPFTDLG
                     TLGFNPDGQLLVADGRGEIQVFDLNGNYLKAIPLVDANGDPIHAGYDVSTDSSGRMYV
                     PDPESNSVYVYDADGKLLLTWSGPGAETPLDPWSVVADSDGNMYVVDVSASAVYKVHL
                     DFH"
     gene            complement(1603..2778)
                     /locus_tag="VHR64_02200"
     CDS             complement(1603..2778)
                     /locus_tag="VHR64_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3301663.1"
                     /translation="MDHPDSFESIVTHGDGGIEETLDVAPPIHQTSTFIAQSAEEFAR
                     QATEARPDAYYTRAGNPTHRRAEAILAKLEGAEAGIVTASGMGAITCSILAHVAAGDH
                     VVAQKNHYMAASKFVTDILPRFGVQSTVVDQTDPDAFAEAIRPETKVIFVETPSNPTG
                     QITDLRAIANLAKQHGITTICDNTFASPINQQPISHGVDIVVHSATKYLGGHHDLIAG
                     AVLGSSEHIETVWRFTNMFGPTLGPIDAWLLLRGLRTLPLRVRHHNEAGCAVARHLEE
                     HSKIEAVHYPGLPSHPDYAVASRQMSGYGATFSFQVRGGYKDTQRFMSNLRMVKQAVS
                     LGGFETLAVHAAAMWGGSLSDEQVREAGVEPNMVRISIGLETAEDVIADLDHALAAV"
     gene            2891..3280
                     /locus_tag="VHR64_02205"
     CDS             2891..3280
                     /locus_tag="VHR64_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3301664.1"
                     /translation="MHFGYTIVYVPDVKAAVAFYEQAFGFKMRFNADDWDYAELETGD
                     TTLAFAAEKMADLFGTSITPNRADATAAGIELAFVTEDVQRAFDRATKSGATAVTEPN
                     QTPWGQTVSYVRDQNGVLIEICSPVSG"
     gene            3625..4635
                     /gene="aroF"
                     /locus_tag="VHR64_02210"
     CDS             3625..4635
                     /gene="aroF"
                     /locus_tag="VHR64_02210"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX3301665.1"
                     /translation="MIVTLAPDVPDGTRSAIADAAALIGSGARELAWEDGVRVVAIDG
                     DLPANILSMPGVAASYDKHKNYMLASKLVRERSLVRIGDVEIGGDHPVIMAGPCVVED
                     RESTIAIAREARAKGAVMLRGGAYKPRTSPYAFQGLGEVGLEHLAAARDDTGLPVVTE
                     VMEPDQVDLVAHYADMLQVGARNMQNYPLLRRVGQSQRPVLLKRGFSATIEEWLMAAE
                     YILAHGNPNVVLCERGIRAFDPAFRFTLDLNAVPMVKELTHLPVVVDPSHGTGKRSLV
                     APMSLAAIAAGADGLILEVHPDPDSALCDGSQTITLDDLGTIARQVVAMHSVLKGALV
                     VA"
     gene            4703..5518
                     /locus_tag="VHR64_02215"
     CDS             4703..5518
                     /locus_tag="VHR64_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3301666.1"
                     /translation="MTIEPEELSRPDRHIFLSPHYDDIALSCGGTAAELSAAGRTPEV
                     ALIFGDHPDPAQPLTGFAQQLHKQWGMGAEEVISGRRAEERMASVALGTCDRFLPFRD
                     AIYRGERYLSDDDLFGAVKADESELPGKIIDELRLCADGAGNTRIYAPLAIGHHVDHQ
                     HAYLTGLELIRRGWEVWFYEDLPYGLKPVNVDARRDQIERDGLKPGPAVAVGSQWKRK
                     IDAIMAYPSQLSTIFGQYVGVGTSRAAIDEAMTAYARTMGDGVPAERFWSRHD"
     gene            complement(5834..6439)
                     /locus_tag="VHR64_02220"
     CDS             complement(5834..6439)
                     /locus_tag="VHR64_02220"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEX3301667.1"
                     /translation="MQALYGRVAITSRRITPGTAARFELRTDQLIQIVTIDGRQAAEM
                     IAFRADEPTERLSTGATRAKNKSIMVEQGKKLYSSRFTPLLEIVTDTVGRHDLLMASS
                     EPTKPAIPNLDGDDALTHPLIEALGEDGVTLDSVLEPVNWFANISIKAKGELEVGESL
                     AEPNDSVVLKALTDLIVVVGARPNMAGTGIAATDLLVRVFR"
     gene            6576..>7639
                     /gene="chrA"
                     /locus_tag="VHR64_02225"
     CDS             6576..>7639
                     /gene="chrA"
                     /locus_tag="VHR64_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX3301668.1"
                     /translation="MTRLALRMGLTAFGGPAAHIAILRHEAVAKRKWLTEQDFLDYIS
                     LANLIPGPNSTELVMHVGAHTGGRRGLWAAGAAFIGPAALITLVFAYIYKRWGSSPTA
                     EGILAGIAPALIVVIIFTLIPLARTAIKSRWAVGLAAALVLLYVTGINELLLLLIGTL
                     LYITPFATRSLKDRTLAVAAPLLLYATAPEQPPPSFVRIFLVFLKIGAILYGSGYVLI
                     AFMQRDVVNGRHWITQQQLVDAVAVGQVTPGPVFSTATFIGYQIGGLGGAIAATIGIF
                     LPAFLLVWATHGVAARFRTSRMLARVLDGLNLASNALLIGVLATLIRALDLTAFNLIV
                     AALALLGLASRRVGPTTVLAG"
BASE COUNT         1371 a         2452 c         2371 g         1445 t
ORIGIN      
        1 ctcccgttgc ttagtgagga tcagcggcgg gtgctcgagc ttcgcctcgc tggactctcg
       61 agcggggaga tcgaaatcgt ccttggcaag agtcgatcct gggtcggcgt tattcagcac
      121 cgggcagtca tccggctgcg agcgttgatg gccgaccgtg ctgagccgaa ggagcgatag
      181 atgcgctggc ttcggaactg gaatcatcgg gatcagaccg acgcgctgaa cgagttctgg
      241 gacgacgtcg tccagcggag ccccgcgacg ccgctgaccc gcgacccggt ccccactgag
      301 ctcggcgcaa tcgttagaca acttcatgcg gagcaggacg cgcggcagca gcgaccggcc
      361 tacgcggatc ggcagctcgc tgtgctcctc tcccaccacc gggaggcaca gtcaatgaac
      421 accttgaaca cctatccatt gcgaaccagt gacgttcggt cggtgccctc tgcgtccgtt
      481 gctggacggt tgttgggcca caaccaacgc cggagctgga taccggcaat atccgccgga
      541 gcggccatcg cgattctgct cgccgtcatt gtggcgctgg cgatcttcgc gaatctcttc
      601 ggaaaccacg acaaccaccc cgtcatcctc gcgccctcga caccaacgtc gcaggcaagc
      661 ccgtcgccca cggtcgaggc cagcctggtc tggacctata cgggcgaggg tgaggacaaa
      721 cttgacaact caacaactgg aacgattgca ccagatggga gtatctgggt ggtcgacgca
      781 gcgaacgcgc gaattcaaat cctcgacccg gatggcagcc tccagcgaat gtacggggtt
      841 gccggcacac gtgacggaca gttcatgttc gacatgggac caggctacgc gggcgaagtg
      901 gccgtcaacc cggacgggag cttttacgtc gccgaagccg ggaacgcccg cgtccagaag
      961 ttcaccgata acgggaagtt cgccgtcagc tggagcggag cgactacgcc gattcaaaca
     1021 ttcagcctcc tgataggagt ctcaatcgat tctgacggca acgtttggac agccgatgag
     1081 tcggccaaca tgattagcaa gttcaccccc gacgggagat tcctgctcgc tattgatggg
     1141 atagacactg cccaacatcc cgagccattc accgacctcg gcacacttgg cttcaatccg
     1201 gacggtcaac tcctggtcgc tgacgggcgg ggcgagatcc aggtgttcga cctcaacggg
     1261 aactacctca aggcgattcc gctggtggac gcgaacggcg atcccatcca cgccgggtac
     1321 gatgtctcga ccgattcatc cggacgcatg tacgtgccgg atccggaatc caactccgtt
     1381 tacgtctacg acgccgacgg caagctcctg ctgacctgga gcggcccggg agcagagacg
     1441 cccctggacc catggagtgt ggtcgcggat agcgacggca acatgtatgt cgtcgatgtc
     1501 agcgccagcg ccgtctacaa ggtccacctc gacttccact gatccgcatg aagccgccgg
     1561 caggggatgt ccctgccggc gactacctcg tttcccagag tctcagactg cagcgagggc
     1621 gtggtcgagg tctgcgatca cgtcttcggc ggtctcgaga ccgatcgaga tccggaccat
     1681 gttcggctcg acgccggctt cgcggacctg ctcgtcgctc agtgatccgc cccacatcgc
     1741 ggccgcatgc accgccaggg tctcgaatcc gccgagcgac accgcctgtt tgaccatccg
     1801 cagattggac atgaagcgct gcgtgtcctt gtagccaccg cgaacctgga aactgaatgt
     1861 cgcaccgtat ccgctcatct ggcgactcgc caccgcgtag tccgggtggc tcggcaatcc
     1921 aggatagtgg actgcctcga tctttgagtg ctcctccagg tgccgggcga ccgcacagcc
     1981 ggcctcgtta tggtgccgaa cgcgcagcgg cagcgttcgc aatccacgca gcagcagcca
     2041 ggcatcgatt ggacccagtg tcggaccaaa catgttcgtg aagcgccaga ccgtctcgat
     2101 atgctcgctc gatcccaata ccgcgccggc gatcaggtcg tgatgcccgc cgaggtactt
     2161 ggtggccgag tgcacgacga tgtcgacgcc atgcgaaatc ggctgctggt tgatcggcga
     2221 ggcaaaggtg ttgtcgcaga tcgtcgtgat gccgtgctgt ttcgcgaggt tcgcaatcgc
     2281 gcgaagatcg gttatctggc cggtcgggtt cgacggggtc tcgacgaaga tcaccttcgt
     2341 ctccggtcga atcgcctcag cgaaggcatc ggggtcggtc tgatcgacga ccgtgctctg
     2401 gaccccaaag cggggcagga tgtcggtcac gaacttcgag gccgccatgt agtggttctt
     2461 ctgggcgacg acgtgatccc cggcagcgac atgcgccagg atcgagcaag tgattgcgcc
     2521 catgccggag gccgtcacga ttcccgcttc cgcaccctcc agcttggcca ggatcgcctc
     2581 ggcccgtcgg tgcgtcggat tgccggcccg ggtgtaatag gcatcgggac gggcttcggt
     2641 ggcttgccgg gcgaactcct cagcggattg ggcgatgaat gtcgatgtct gatgaatcgg
     2701 aggggcgaca tcgagcgtct cctcgatacc gccgtcgccg tgcgtaacga ttgattcgaa
     2761 cgagtccggg tggtccacat ttcctgcctt tgatccgatg cgtccgcatt gacgcggcat
     2821 cagtctatct tgtgcgacga ctcgaagtgt cgtacattcg ttcgacttac tcagggagaa
     2881 ggaggcagag atgcattttg ggtacacgat cgtttacgtt ccggatgtca aggcggccgt
     2941 tgcgttctac gagcaggcgt tcgggttcaa gatgcggttc aacgccgacg actgggacta
     3001 cgccgagctc gaaaccggcg acacgacact cgccttcgcg gccgagaaga tggccgatct
     3061 gttcggtacc tcgatcacgc cgaaccgggc ggatgcgacc gcggccggaa tcgagctggc
     3121 cttcgtgacc gaggatgtcc agcgcgcgtt cgatcgggca accaaatccg gcgccacggc
     3181 agtgaccgag cccaaccaga ccccttgggg ccaaaccgtc tcgtatgtca gagatcagaa
     3241 cggcgtcctg atcgagatct gctccccggt cagcggatag tggatggtga ttagtggcta
     3301 gaaccgattt acccactaac cactatccac taatcactaa ccactccctc ggcggcttga
     3361 cgcctcgttc cgccccgtgt agtgttcgat ttatggaaca ggttaatgcg cgagctggat
     3421 ctgacctgga gctggtggcg gccgagcatg gccccactgc gggcttctgg ttttatttcg
     3481 gcttttattg ggactccccg gcgatgctgg cggagcgaga gtcgcgcatg agatagaccg
     3541 ctccaactca agccacatcg accggggacc cgaggcaagc agcctcggtt ttttgttttc
     3601 tgaaatcgga cgaggagaac cgccatgatc gtcacactcg caccagacgt acccgacggc
     3661 acccgatccg ccatcgcgga cgccgccgcg ttgatcggca gcggagcacg cgagctggca
     3721 tgggaggacg gcgtccgcgt agtggcgatc gatggcgacc tcccggcgaa catcctgtcc
     3781 atgccaggcg ttgccgccag ctacgacaag cacaagaact acatgctggc aagcaagctg
     3841 gtgcgcgagc gcagtctcgt ccgtattggc gatgtcgaga ttggcggcga ccacccggtc
     3901 atcatggcgg gaccgtgcgt ggtcgaggac cgcgagtcaa caatcgcaat tgcccgggaa
     3961 gcgcgggcca agggcgccgt catgctccgt ggcggcgcgt acaagccgcg cacctcgccg
     4021 tatgcattcc agggattagg cgaagtcggt ctcgagcacc ttgcggccgc acgggacgac
     4081 accggcctgc cggtcgtgac ggaggtcatg gagccggatc aggtcgacct ggtcgcccac
     4141 tatgccgaca tgctccaggt cggcgcacgc aatatgcaga actacccgct gctccgccgc
     4201 gtcggtcaga gccaacgccc ggtgctgctc aagcgcggct tctcggccac catcgaggaa
     4261 tggctgatgg cggccgagta catcctcgcc cacggtaacc cgaacgttgt cctctgcgag
     4321 cgcggcattc gcgccttcga tcccgcgttc cggttcacac tcgacctgaa tgccgtgccg
     4381 atggtcaagg agcttacgca cctgccggtc gtcgtcgatc ccagtcatgg cacaggcaag
     4441 cgctcgctcg ttgctccaat gtccttggcg gcgatcgcgg ccggcgccga tgggttgatt
     4501 ctcgaggtcc acccggatcc cgactccgcc ctctgtgacg gatcgcagac gatcacactc
     4561 gatgacctgg gcacgatcgc acggcaagtt gtcgccatgc attccgtact gaaaggcgcc
     4621 ctggtcgtcg cgtgacatag tgacgcgtgg catacactgg aggcatgacg atccgcgcgc
     4681 ttctgcagaa ggacggcctg ccatgacgat cgagccagag gaattgtccc ggcccgaccg
     4741 gcacatcttc ctgtcgccgc attacgacga tatcgcgctg tcctgtggcg ggacggcggc
     4801 cgagctttcc gccgccgggc gaacgccgga agtcgcgctg atctttggcg accatcccga
     4861 tcccgcgcag ccgctgaccg gcttcgccca acagctccac aagcagtggg gcatgggcgc
     4921 ggaagaggta atttccggca ggcgcgccga ggagcgcatg gcctcggtcg cgctcggcac
     4981 ctgtgatcgc ttcctgccgt ttcgggatgc catctatcgc ggggagcggt acctgagcga
     5041 tgacgatctc ttcggcgccg tgaaggcaga tgagtccgaa ttgccaggca agatcatcga
     5101 tgagctccgg ctttgcgcgg atggcgcggg caacacgcgg atttacgcgc cgctcgcgat
     5161 cgggcatcac gtcgatcacc agcacgccta tttaaccgga cttgaactga tccgccgcgg
     5221 atgggaagtc tggttctatg aggatttacc ctatgggctc aagccggtga acgtcgacgc
     5281 gcgccgggat cagatcgagc gcgacggtct gaagccgggt ccagccgtcg cagtcggcag
     5341 ccagtggaag cggaagatcg acgcgatcat ggcctaccca tcgcagctca gcacgatctt
     5401 cggccagtac gtcggcgtcg gcacctcgcg cgccgcgatc gacgaggcaa tgactgccta
     5461 cgcgcggaca atgggcgatg gcgtgccggc cgagcgattc tggtcgcgcc atgactgagc
     5521 ttcgcgaggc caggcgcacg ctcatcctga tcggtgtcgc gatcctgatc gtctgggcaa
     5581 tcctcttccc actcgtattc ttctagtgtc gccgtcctgg taacgcgaaa aaccgtacgg
     5641 agcgcggggt taccacgccc cacttggtgc atccccacgc gcggttgatg gatgcgatca
     5701 gggtcccggg ttagttcctg catcgagccg gtcgtcatcg agcccggacg gggcgtgttc
     5761 ctctctccga ctctctccgc aaggaaagag ggcatccccg cgctactaat tgatcggaat
     5821 acgcctcgcc tggttaccgg aagacgcgga cgagaaggtc ggtggcggcg atgccggtgc
     5881 cggccatgtt cggacgcgcg ccgacgacga cgatcaggtc ggtcagcgcc tttagcacaa
     5941 ccgaatcgtt cggctccgcg agcgactcgc cgacttccag ctcgcccttg gccttgattg
     6001 agatattcgc gaaccagttg acgggctcga ggaccgaatc cagcgttacg ccatcttcgc
     6061 ctagtgcctc gatcaacggg tgcgtcagcg catcatctcc gtcgaggttt gggatggccg
     6121 gcttggtcgg ctcgctcgac gccatcagca ggtcatgccg gccgacggtg tcggtgacga
     6181 tctccagcag cggcgtgaag cgggacgagt agagtttctt accctgctcg accatgatcg
     6241 acttgttctt cgcccgagta gcaccggtcg aaagccgctc ggtcggctca tccgcgcgga
     6301 aggcgatcat ctcggccgcc tggcgaccgt cgatcgtcac gatctggatc aactggtcag
     6361 tccgcagctc gaacctggcc gcagtgccgg gtgtgatccg ccgcgaggtg atcgcaaccc
     6421 gtccatataa cgcttgcacc gatcgacctc ccagtcgagc acgctccgaa taggcaaccc
     6481 ggcaattgtc gcagccatct cggctctgat tcaattcccg ccgaagccgg attgtacgca
     6541 ggtccaggag aacaccgctg ccagcgctga gcgacatgac cagattggcg ctgcgaatgg
     6601 gactgacggc cttcggcggt ccggccgcac acatcgcgat tctgcggcac gaggctgtcg
     6661 caaaacgcaa gtggcttacc gagcaggatt ttctcgacta catcagcctg gccaatctga
     6721 tcccggggcc gaactcgacc gagctcgtga tgcacgtcgg cgcgcacacc ggcggccgac
     6781 gcggactttg ggcggccggc gcggctttca tcggtccagc cgcgctgatc acgctcgtct
     6841 tcgcgtatat ctacaagcgc tggggatcgt ccccgaccgc tgagggcatc cttgccggga
     6901 tcgcgccggc gctgatcgtc gtgatcatat tcacgttgat tccgctcgcc cgaaccgcga
     6961 tcaagagccg ctgggcggtc gggctggccg ctgcactcgt gctcctctac gtcaccggta
     7021 tcaacgagct gctcctgctg ctgatcggca cgctgctcta catcaccccc ttcgcgaccc
     7081 gctcgctgaa ggaccgcacg ctcgctgttg cggcccccct cttgctctac gcgaccgccc
     7141 cggagcagcc accgccgagt ttcgtccgca tctttctggt cttcctcaag attggggcaa
     7201 tcctctacgg gtcggggtac gtcctgatcg cattcatgca gcgggacgtg gtcaacggcc
     7261 ggcattggat cacgcagcag cagttggtcg atgccgtcgc ggtcggtcag gtcacgcctg
     7321 gtcccgtctt ctctaccgcg acgttcatcg ggtatcagat cggtgggctt ggcggggcga
     7381 tcgcggcgac gatcgggata ttcctgccgg cctttctact ggtttgggcg acccacggtg
     7441 tcgcggctcg attccgaacg tcgcgaatgc tcgcgcgcgt gctcgatgga ctcaatctcg
     7501 cctcgaacgc gctcctgatc ggcgtgctgg ctaccctgat ccgcgccctc gacctgaccg
     7561 ccttcaacct gatcgtcgcc gcgctggcat tgctcggact cgcctcccgg cgggtcggtc
     7621 cgacgaccgt tttggccgg
//
LOCUS       DATAOZ010000077         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7924804, whole genome shotgun sequence.
ACCESSION   DATAOZ010000077 DATAOZ010000000
VERSION     DATAOZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7924804"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            654..>1839
                     /locus_tag="VHR64_02230"
     CDS             654..>1839
                     /locus_tag="VHR64_02230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX3301669.1"
                     /translation="MHTQDVVLCEPVRTAIGTYNGSLKGVPATELGATAIRETLSRAG
                     LDGAHIDTVVMGNVIQAGNRMNPARQAAVGGGLPVSVPAMTVNRVCGSGAQAIVTAAQ
                     EILLGQADATIAGGMENMDRAPYLVDGGRWGYRMGHAQIYDSVLLDGLNDAFSGEHSG
                     WHTEDLVTRMQITREAQDRWAERSQQRFGAGQAAGKFDPEIVPVGIKTRKSVEQFRRD
                     EAPRPETTTATLAKLRPAFRDDGSITAGNAPGLNSGASAMIVASRAFAEAKGVQPFAR
                     LVAYGVAAVEPGMFGLGPVPAVRQALERANWRLDSIERIEINEAFAAVPLAVSQELGL
                     AEDIVNPEGGAIAHGHPIGATGAVLTTRLLHAMRRDGLRRGIVTLCIGGGQGIALALE
                     AVA"
BASE COUNT          339 a          596 c          591 g          313 t
ORIGIN      
        1 aggatcagga tactccacca ttccccgacc cgctccaggc tgcgggcgat cggacatggc
       61 atgtcgccga agctcttgcg ctgcatgtcc ccaatatcgg ctggtttcta gcaaaatgca
      121 agtctcaccc cggcggccgc cgcctccatg cacgcctcct gacgctgccc agcggattct
      181 gcagaccttg taacttgcat tcaatagccg cgggagcagc ctcttacata tcgatagtga
      241 atagcctgga gccaaggact acgcgatgaa tgatcgagag ctcgaacggc ctccgtcctt
      301 cctgcatcgg gatgtcatcg cccccaaagc gcgcgctcga ccgcaccgat ccgcattcct
      361 gggaatcccg gagcatctga acagcagcaa ccctgcggcc tcggcgtggc tcaaccgggt
      421 ctcgacggcg cggagcgtcg ggttcggggg ccatgtcgcc cacggtcacc ccgcggcgct
      481 gaaagctctg gggccgctct cgttgcgcca gcgcagacgc aaacgtaggc cgatgcctta
      541 ttcgtcatca tgctctgctt cgccatcgct gcccgccatg gtgcaatcga tgcgtaagat
      601 cgtcccaatg gccgaaccgt cagccgacgc acactgagaa caggagaact caaatgcaca
      661 cccaggacgt tgttttatgc gaaccggtcc gcaccgcgat cgggacctat aacggctcgc
      721 tcaagggcgt gcccgcgacc gagctcggag cgaccgccat tcgggaaacg ctcagccggg
      781 caggccttga cggcgcgcat attgatacgg tcgtcatggg caacgtcatc caagcgggta
      841 accggatgaa tccggcgcgg caggcggccg tcgggggcgg attgcctgtc tccgtgccgg
      901 cgatgaccgt gaatcgcgtt tgcggatccg gcgcccaggc cattgtcacc gccgcccagg
      961 agattttgct gggccaagcc gatgcgacaa tcgccggtgg catggagaat atggatcgcg
     1021 caccgtatct cgtggacgga ggacgctggg gctacaggat gggtcacgcc cagatctatg
     1081 atagcgtgct ccttgatggg ctgaacgacg ccttctccgg tgagcattcg ggatggcaca
     1141 cggaggatct cgtcacccgg atgcagatca cgcgcgaggc ccaggaccgg tgggcggaac
     1201 gttcccagca acgattcggc gcgggacagg cagccggaaa gttcgacccc gagatcgttc
     1261 ccgttgggat caagacccgc aagagcgtcg agcagttccg tcgcgacgag gcgcctcgac
     1321 ccgaaaccac caccgccacc ctggcaaaat tgcggccggc gttccgcgat gatggcagca
     1381 tcaccgccgg caacgcgccc ggacttaact ccggcgcatc ggcgatgatc gtggcgtccc
     1441 gggcgtttgc cgaggccaag ggcgttcagc cgttcgcgcg cctggtggcc tatggcgtcg
     1501 cggccgtcga gccgggtatg ttcggcctgg gtccggtgcc cgccgttcgc caagcgcttg
     1561 aacgcgcgaa ctggcgactg gacagcatcg aaaggatcga gatcaacgag gcgttcgccg
     1621 ccgtgccgct cgctgtgtcg caggagcttg gcctggcgga ggacattgtc aatccggagg
     1681 gcggagcgat tgcgcatgga catccgatcg gcgcgactgg agccgtgctg accacccgac
     1741 tgctccacgc aatgcgacgc gacggcctgc ggcgcggcat cgtgacactg tgcatcggcg
     1801 gcggccaggg catcgcattg gcgttggagg cagttgcat
//
LOCUS       DATAOZ010000078        14284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1086144, whole genome shotgun sequence.
ACCESSION   DATAOZ010000078 DATAOZ010000000
VERSION     DATAOZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14284
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086144"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1513
                     /locus_tag="VHR64_02235"
     CDS             <1..1513
                     /locus_tag="VHR64_02235"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX3301670.1"
                     /translation="ARAAAAVSQLQIAAAQIQHETNVFSSVRTDMAAFQQSGLKLGPE
                     IEAERGTNSVFGGFFAAAERHDVDLLPILSVWVTPSGIVSADAITELVTHLIEQLIAS
                     QPDAVALSLHGAMVSEIDRDGDGWILDQIRETVGWDIPVVAELDLHANISTRMIEAAD
                     ILVGYDTYPHVDMAERGAEVIDLLVRMLRGEINPAMAMVKPPMLPTSQRMMTAHDPMA
                     KIMRRAHEIEADPRVVNVTIAGGFPPSDAEEGGVSCVVTTNGDLTLAIDYAEELARLA
                     WSVRDGFLGGVTSFEDAAAAIAALPEHPEQPLLIVDIADSIWSGSSGDSVELIRFFLK
                     RQVIRAAVAPVVDPEVVQKAQTAGIGATIDIDLGGKIDKLHGDPLLCSATVLNLSDGR
                     YVNSGPMMTGLQVDMGPTALLSIGDPAVRVVVTSFPEAPIDAAVFTANGIGLASTRVL
                     GLKGKGHFRAAFEPIVSDVILVEGPGVTGSDLTRLPLRHSRRPIWPLDDVPFD"
     gene            1573..2487
                     /locus_tag="VHR64_02240"
     CDS             1573..2487
                     /locus_tag="VHR64_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX3301671.1"
                     /translation="MRELHQPNGEFSNLFSPYLDPVATIQQGETVTIHTVDAFANVLT
                     GEDTLPSKTPRKYWNPQTGPFYIDGAEPGDTLAVKIENIEATRDWAISASIPYFGGLS
                     STPLTRMLQPPLEERVWIYNRNAAGNWAYKDKWEVPWAPFFGTIGTAPKLEALSSLSP
                     GDHGGNMDVPDVTIGNTVYLPVQHPGALFYTGDCHAAQGQGELCGVALEIAAKGTFTF
                     NVIKGKAIQWPRIESPDRIMVVGSARPMEDAARIAYGSLIDWMVSDYGWDMLDAYQLL
                     TQVGGCYVGNMVDTTYSMVASVQKSYLT"
     gene            complement(2583..3764)
                     /locus_tag="VHR64_02245"
     CDS             complement(2583..3764)
                     /locus_tag="VHR64_02245"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3301672.1"
                     /translation="MVDFATTRVWDVHAHPFLDRGPISPDDFVRLTAFGGGAEEYFEQ
                     GGIEPTAEVSAEIQQWKRQTTWHKLLVRELADYFGVERSLDTVVAARNEAVANGYRDY
                     VGKLYESAQIDGIIFDDGYPLPQIPMDKVREQIPVSCKPIYRIEPLIVELLKQDLSWP
                     EFRQKFDDTISNALTSGGYVGVKSVIAYRTGLDISPLSRTPDQGFQALDAVKRGLGGG
                     SMKKLRDHLLCRSLELCTEHGVPMQIHTGMGDFEVNLILARPAYLMDLLRFPAYRACT
                     VILVHTGYPYHREAGYMAHVLPRVYCDISEGVPFAGSGAAAILRELIEMAPLHKISYG
                     SDAYSVPEGIFASAKIGKRVVTRVMTELVADDVLTEAEAQDVGEMILSGTTKRIYNQG
                     G"
     gene            complement(3765..5105)
                     /locus_tag="VHR64_02250"
     CDS             complement(3765..5105)
                     /locus_tag="VHR64_02250"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX3301673.1"
                     /translation="MTEEELLGRLAQDQIDYLWVAFHDYNGRACAKSNPRGSMSSAVR
                     TGMVFAKANLNMDLSDHQVSGATMLADAGDFMAVPDPRSYAQLPRYPRTALTHSYMRE
                     PDGSPWDGCPRTRLDTIVGKLAEEGFSVQSALEPEFYLFVRATDGELEPITGSTMYGQ
                     RGLSDANEFVVDLLSELAGMGIEVAQFHKEYGRGQYELSLQHGSPIQAVDRYLMFRAT
                     LHDIAHKHGMVATMMPKPTADSPGNSLHVHLSLWDAGGGENLTASVEDDISLSQTALW
                     FTGGLLAHAPALTAVGSPSVNSYKRLLPGSWAPANAYWGAGNRSGLIRVPGPGSRRRI
                     EFRSSDNTAQPYLLMCALLAAGLDGIMRQIDPGPAFAGDVGHMSSAEIEREHVSFLPR
                     TLPGALDALEQDELVCAALGPEILKHFLAVKRGELATYNTVVHEWERSMYLELQ"
     gene            complement(5109..6287)
                     /locus_tag="VHR64_02255"
     CDS             complement(5109..6287)
                     /locus_tag="VHR64_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX3301674.1"
                     /translation="MSFDSRITTYLQIARDRYIPELTRVCAIDSGSYHKVGIDQVQDH
                     FQRRLAELGFDVERVRNAEWGDDLVARRRGTGKGRILMIGHADTVYPVGEAARRPVVI
                     EGDHLLGPGTCDMKAGILTGIYAVEALNAIGWTDYELLTILIVSDEEIEQRHSVELLR
                     SEGAAHDVVLTLEAARANGDIVTARKATRWFRIDAKGKAAHAGVEPEKGASATLALAN
                     VIVESFKLNGRTPGMTLNPGKIAGGANPNIVAAEASVLVDIRAWTNQELEELAGALQA
                     IVDTEWVPGVTQTMSLNGGAGMPAMERTPGTQRLEELAIRIAGQLGFSLKGASTGGGS
                     DVSIAVHAGAAGLDGLGPIGGLDHSPDEYIEVSSIVPRTALLTGLLRAICADESLTRR
                     "
     gene            complement(6291..7640)
                     /locus_tag="VHR64_02260"
     CDS             complement(6291..7640)
                     /locus_tag="VHR64_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3301675.1"
                     /translation="MVRAEPVTSSAIIERDNQFVADAIKIRYTPFVLAGGEGTRLFDA
                     EGNSFLDFSSLWSLANLGYSDKTVRQAIKDQIDRTQCSVLASAFNEPGGLLAERLAGM
                     MSRQPKKVWFGLSGSDASEAAQRLLLKATGKRRLISFIGSWHGTTDATMALSGFAGFN
                     DRFGGSNIVKIPYPDPYRRPFLPHPDQSLTDQCLSYLEDVLFATICPPDDVAGIFVEG
                     VQADSGDIVPPDDFLPKLRSLCDRYGIYLVIDDVKIGLGRTGRMFSYEHAGIEPDIVL
                     LGKALGAGLPLSAIIGPPEVLDIGTGLALYTISGNATSCAAALAGLDVVEREGLVEQA
                     DRVGAYLLRRLDTLMHHDQVGDVRGRGMICGVDLVTDRESKKPDSALAARVVYRAWQL
                     GLIVFYAGTYSNVLEITPPLILTEAEVDEGVAILDQAIDDAVNGRVDEAAVAAYAGW"
     gene            complement(7713..8978)
                     /locus_tag="VHR64_02265"
     CDS             complement(7713..8978)
                     /locus_tag="VHR64_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3301676.1"
                     /translation="MSNSERSDSVSRLIHDSIDRRVNRRDILRRGAELGVAAPMLAAM
                     IKVSETTTVFAQDAGEIVVGCPYNLTGSLQSIDVPAKDGSLLAAKLLNASGGVLGKQL
                     KLVVENGESDVTTVSNICKKMVDEDKVIALVGLTDTDYVRAGGQVAQEKGIPFIDVGG
                     TAPIIAQIGDFMFMLPFGDNVQAAVEAEFAQEKGWKTAAMLVNDGMTYTKFLAQYFKE
                     RYTMDDIGGQVIKELTYKVGDTDFSSQVTEIKGLNPAPDVLMISAGPEEIGTIVKQVR
                     EGGIETPILGGDGYDTPQLLELAGANARNVFFTTHEGIYGDQSDAAKKFNDEYNKEYG
                     KAPDSIFAALGYDGINLLADGITRANDTAGDKIRDALAATTGFKGATGEISYTKDSHI
                     PTKSVALVEAKDGKFELIKTTVPKTVPKA"
     gene            complement(9083..10138)
                     /locus_tag="VHR64_02270"
     CDS             complement(9083..10138)
                     /locus_tag="VHR64_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3301677.1"
                     /translation="MARAAVYGAALLVLAGFLWIAQDTFTSYRIQLVVLVAINIVVAV
                     SLTMSSGFTGVFSLGQVGFMAIGAYVGALISLPLTWKDPILLPGLPSWLAEFDTSGWP
                     DWLALAFACIVGGVIAMIVAVVVGYPLMRLSGNYVAVATMGFLIIVYTVLIQWDSVTF
                     GSQGLNPIPTYTNVWSAYFWAVLTIYVAWRIRRSSFGRSMIASRDNLIAARAVGVRVH
                     RARLLAFTVCGFLTGVGGCLLGHQIGTVSPNSFYFTTTFNIIIMVVLGGLGSITGAIF
                     GAIFMTLAPEVLRNLETNVTIGPIHWDQAFGLSQIILALIFIAVMILRPSGLMGSREI
                     DLAGWLGKRQAGSDRAQ"
     gene            complement(10139..11035)
                     /locus_tag="VHR64_02275"
     CDS             complement(10139..11035)
                     /locus_tag="VHR64_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3301678.1"
                     /translation="MSGTEFGQQFINALSLGSIYALVAIGLAMVFSILRLINFAHGEV
                     LMLGAFFTYYAERAGFPFLPAAIIGVGGTIIAGLLMERVAYRPLRGAPEISLLLTSLG
                     VSIAVRNSASMIFGVQPKKFPTPGSFEERVGIGGGVRISVTNIITIAVALILLALLTW
                     FVTRTRLGIQMRATAENPVAARLMGINTNTVIVSAFIIGSALAGVAGVLWGARVGRIE
                     PLMGFVPVLKAFVATVIGGFGSIPGAVLGAFILGFGEIFLVALLPDHLTGYRDAFIFA
                     ALIVILLVRPQGILGSTEPEKI"
     gene            complement(11032..11748)
                     /locus_tag="VHR64_02280"
     CDS             complement(11032..11748)
                     /locus_tag="VHR64_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3301679.1"
                     /translation="MLKVDNLRVNYGAIQAVKGITFEVEERELVALIGSNGAGKSTTL
                     KTVSGIVRPSGGSITYQGRDLSRMPTHQIVSSGIVQAPEGRQIFGSLTVRENLMLGAS
                     ARSDRDGFDADLQRVFDLFPILGERISQRAGTLSGGEQQMLAIGRALMGKPKLLLLDE
                     PSLGLAPLIVNRIFDVIRQLKETGVTILLVEQNARKALAVADRAYVLETGLISLSGAA
                     AELARNSAVEQAYLGGGARA"
     gene            complement(11748..12572)
                     /locus_tag="VHR64_02285"
     CDS             complement(11748..12572)
                     /locus_tag="VHR64_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3301680.1"
                     /translation="MVDHQIADANPVVLQAADVGRSFRGLKALSGYSVSLRKGEILGV
                     IGPNGAGKTTLFNVITGFVPPTDGQIDLNGISIIGKAPDAILRAGIARTFQNIRLFSG
                     MTVLENVLTAQQVRQPMGLFQTLLSLPGFLGNERRMREAAISHLREVGMDRYADQPAT
                     ALPYGMQRKLEIARAIATSPAILLLDEPAAGMNEQETAELTGFIREIREKFDLTIIVV
                     EHDMSLIMRLCDRIQVLNYGSIIAEGTPTEIQTNPAVIEAYLGAAEEEPAPLGEVG"
     gene            12668..13276
                     /locus_tag="VHR64_02290"
     CDS             12668..13276
                     /locus_tag="VHR64_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301681.1"
                     /translation="MRRLLLLLLIVMTLGAISAADAVAAPASATPETERCTVDPLSPE
                     HLTAIVAAGFEPTPRLEKGDQAVPATDLVAMFDVLTESVACTNANQPMRALALYTDRY
                     LAERFSGQAGEDELGHLIAASSRSPKPAAPGDQLVLLAVADPILYGDGRIGATVTTAN
                     AEATFIDQIIFAKTEEGWRIDQVVLGKEQHATPAATPGSTQP"
     gene            13316..>14284
                     /locus_tag="VHR64_02295"
     CDS             13316..>14284
                     /locus_tag="VHR64_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX3301682.1"
                     /translation="MPKSHVDRSTTAPHPEPTIEVPADELAALKEIERRILWLSTLMI
                     HHANNVRPSLEKSKVGGHQASSASVASIITALYFHFLRGGDRVSIKPHASPIYHAAHY
                     LLGNLDRKYLTTLREYKGLQAYPSRTKDPDPVDFSTGSVGLGAVAPVFAALAHKYAEL
                     HFGEVTSNRFISLIGDAELDEGNVWETVAEEAIRGLGNVIWIVDLNRQSLDRVIPGVR
                     AGRLKALFAGAGWRVFETKYGSTLQAAMDTSPNDALRRRIDEMSNEEYQALIRLRDGA
                     EMRRRLADVPQSADSKDILLSVAGIPDEGLGHVISNLAGHDLPRLLE"
BASE COUNT         2635 a         4715 c         4410 g         2524 t
ORIGIN      
        1 tgctcgggct gccgccgctg tgagtcaatt gcagatcgca gcggcgcaga ttcagcacga
       61 gacgaatgtc ttcagctcgg tccggaccga catggccgcg ttccagcaat ccgggctgaa
      121 attgggcccg gagatcgaag ccgagcgcgg cacgaattcg gtcttcggcg gcttcttcgc
      181 ggcggctgag cggcacgacg tcgacctgct gccgatcttg tcggtctggg tgacgccgtc
      241 cggcatcgta tcggccgatg ccatcaccga gctggtcacg catctgatcg agcaactgat
      301 tgcgtcgcag ccagacgctg tcgccctctc cctgcatggc gcgatggtta gcgagatcga
      361 ccgtgacggc gacggctgga tcctcgacca gatccgggag acggtcggct gggacatccc
      421 ggtcgtggcc gagctcgatc tccatgccaa tatctcgact cgcatgatcg aggcggcgga
      481 catcctggtc gggtacgaca cgtacccaca tgtcgacatg gccgagcgcg gggccgaggt
      541 catcgacctg ctcgtccgga tgctgcgcgg cgagatcaac ccggcgatgg cgatggtcaa
      601 gccgccgatg ctgccgacca gccagcggat gatgaccgcg catgacccga tggcgaaaat
      661 catgcgccgc gcccatgaga ttgaagccga tccgcgcgtg gtcaatgtga ccatcgccgg
      721 tgggtttccg ccgtcggacg ccgaggaggg cggcgtgagc tgcgtcgtca cgaccaacgg
      781 cgacctgacc ctggcgatcg actacgctga agagttggcg cggcttgcct ggtcggttcg
      841 ggacggcttc ctgggcggcg taacatcatt cgaggacgcg gccgcggcga tcgcggcatt
      901 gcccgagcac ccagaacagc cgctcctgat cgttgacatc gccgatagta tctggtccgg
      961 cagctctggg gacagcgtcg agctgattcg attcttcctc aagcgtcagg taatccgcgc
     1021 ggcagtggca ccggtggtcg atcccgaggt cgttcagaag gcacagaccg caggaatcgg
     1081 cgcgacgatc gacattgacc tcggcggcaa gatcgacaaa ctccatggcg atcccctcct
     1141 ttgctccgcc accgtgctta acctgtcgga cggacgttac gtcaactccg gtccgatgat
     1201 gaccgggttg caggttgaca tgggaccgac cgcgttgctg tccatcggcg acccggcagt
     1261 tcgcgtggtc gtgacgtcct tccccgaagc gccgatcgat gcggccgttt tcacggcgaa
     1321 cggcatcggc ctggcatcga cgcgcgtgct cgggctaaag ggcaagggcc acttccgcgc
     1381 cgccttcgaa cccatcgtca gcgatgtcat ccttgtcgaa gggcccggcg tgaccggttc
     1441 cgacctgacc cgtctcccgc tccgccacag tcgccgcccg atctggccgc tcgacgacgt
     1501 cccgttcgac tgagcgcgac tgctcgccgt ctcctaagat gacgccatcg gacgttgctc
     1561 atggagggaa cgatgcgcga gctgcaccag cccaatggcg agttttctaa cttgttcagc
     1621 ccctacctgg acccggtcgc gaccatccag cagggcgaga cggtcacgat ccacaccgtc
     1681 gatgcgtttg ccaatgtcct gaccggcgag gacacactgc catcgaagac gccgcggaaa
     1741 tactggaacc cccagactgg tcccttctac atcgacggcg ccgaaccggg cgacaccctg
     1801 gccgtgaaga tcgagaacat cgaggcaacg cgcgactggg caatctccgc ttcgatcccc
     1861 tactttggcg gtctcagctc cacaccgttg acccgaatgc tccagccgcc gctggaggag
     1921 cgggtctgga tctacaatcg gaacgccgcc ggaaactggg cctacaagga caagtgggag
     1981 gtgccgtggg cgccgttctt cgggacgatc ggcacggccc caaagctgga ggcgctctcc
     2041 tcactctcgc ccggcgatca cggcggcaat atggacgtcc cggacgtgac gatcggcaac
     2101 acggtctacc tgccggttca gcacccgggc gcgctcttct acacgggtga ctgccacgcg
     2161 gcgcaaggac agggcgagct ctgcggcgtc gcgctcgaga ttgcggccaa aggcaccttc
     2221 accttcaacg tgatcaaggg caaggcaatc cagtggcccc gcatcgagtc gccggatcgg
     2281 attatggtcg tcggcagcgc ccggccgatg gaggatgccg cccggatcgc ctacgggtct
     2341 ctgatcgact ggatggtcag tgattacggc tgggacatgc tcgacgccta ccagctcctg
     2401 acccaggtcg gcggctgcta cgtgggcaac atggtcgaca ccacctactc gatggtcgcc
     2461 agcgtgcaga agagttacct cacgtaacag tgacgcccct ggcggggtga cctgccaggg
     2521 gcgctatctg ggccgcggcg caggttgggc gaccgcggtg agggggatga gctcgtgcgg
     2581 gttcatccgc cctgattgta aatccgtttg gtggttccgc tcaggatcat ctcgccgaca
     2641 tcctgcgcct cggcctcggt caggacatcg tcggcgacaa gctcagtcat gacccgagtg
     2701 acgacgcgct tgccgatctt tgctgaggcg aagatccctt cggggacact gtaggcatcc
     2761 gagccatacg agatcttgtg gagcggcgcc atctcgatca gctcgcgcag gatcgccgcc
     2821 gcgccactcc cggcgaacgg cacgccctca ctaatgtcgc agtacacccg cggcagcacg
     2881 tgggccatgt aaccggcctc acggtggtac ggataaccgg tgtggacgag gatcacggta
     2941 caggctcgat aggccgggaa gcgcagcagg tccatcagat acgccggccg ggcgaggatc
     3001 aggttaacct cgaagtcacc catcccggtg tggatctgca tcggcacgcc gtgctcggtg
     3061 cagagctcga gtgagcgaca gagcaggtga tcgcgcagct tcttcattga gccgccgccg
     3121 agtccgcgct tgaccgcatc cagcgcctgg aatccctggt caggcgtccg gctcagaggc
     3181 gaaatgtcga ggccggttcg ataggcgatg accgacttga cgccgacgta ccctccggat
     3241 gtcagcgcat tcgaaatcgt atcgtcgaac ttctgccgga actcgggcca cgagaggtcc
     3301 tgtttgagca gttcgacaat gagcggctcg atccggtaaa tcggcttgca actgaccggg
     3361 atctgctccc gaaccttgtc catcggaatc tgcggcagtg gatagccgtc atcaaagatg
     3421 atgccgtcga tctgggccga ctcgtagagc ttgccgacgt aatcccgata cccgttcgcg
     3481 accgcctcat tgcgcgccgc aacgaccgta tccagcgagc gctcgacccc gaagtagtcc
     3541 gccagctccc ggaccagcag tttgtgccag gtcgtctgcc gcttccattg ctgaatctcc
     3601 gccgagacct cggcggtcgg ctcgatgcca ccctgctcga aatactcctc cgcccctcca
     3661 ccgaacgcgg tcagtcggac aaaatcgtcg ggcgagatcg gtccgcggtc gagaaacggg
     3721 tgtgcatgga catcccagac gcgagtcgtc gcgaaatcaa ccatttactg cagctccagg
     3781 tacatggatc gttcccattc gtgaaccacc gtgttgtagg tcgccagctc gcctcgcttg
     3841 acggcgagga agtgtttcag gatttcaggc ccgagcgccg cgcagacaag ctcgtcctgt
     3901 tcaagcgcgt cgagggctcc cggaagcgtt cgcggcagga agctgacgtg ctcgcgctcg
     3961 atttcggccg agctcatgtg accaacgtcg ccggcgaagg ccgggcccgg gtcgatctgg
     4021 cgcatgatgc cgtccaaccc agccgccagc aacgcgcaca tcagcaggta cggctgcgcg
     4081 gtattgtcgg acgagcggaa ctcgatcctt cgccgcgatc ccggccccgg aacccggatt
     4141 aggcccgagc ggttgccggc tccccagtag gcattggcgg gcgcccagga gccgggcagg
     4201 agccgcttgt aactgttcac ggatggtgac ccgactgcgg ttagagctgg ggcgtgggcg
     4261 agcaggccgc cggtgaacca caaggccgtc tgactgaggg atatgtcgtc ttcaacggac
     4321 gccgtcaggt tctcccctcc gccggcgtcc cataggctca aatgcacgtg cagactgttg
     4381 cccggcgagt ccgccgtcgg cttcggcatc atcgtcgcaa ccatgccgtg cttgtgggct
     4441 atgtcgtgca atgtcgcccg gaacatcagg taacgatcga ccgcctggat tggcgaccca
     4501 tgctgcagcg acagctcata ctggccgcgc ccgtactcct tgtggaactg cgctacctcg
     4561 atccccatcc cggccaactc gctcaagagg tcgacgacga actcgtttgc gtcggagagt
     4621 ccccgctgcc cgtacatggt cgatccagtg atcggctcaa gctcgccatc ggtcgcccgc
     4681 acgaataggt aaaactccgg ctcgagtgcg gactgaaccg agaaaccctc ctcggccaac
     4741 ttgccgacga tcgtgtcgag ccgggttcgc ggacagccat cccacggcga cccatctggc
     4801 tcgcgcatgt acgagtgcgt caacgcggtc ctggggtagc gcgggagctg tgcgtaactg
     4861 cgcggatcgg gcaccgccat gaaatcaccc gcgtcagcca gcatcgttgc cccgctgacc
     4921 tggtggtcac tgagatccat gttcaggttc gccttggcaa acaccatgcc ggtgcgaacc
     4981 gcactggaca tgctccctcg tggattcgac ttcgcgcagg cgcggccgtt gtagtcgtga
     5041 aacgcgaccc agaggtagtc gatctggtcc tgcgccaatc ggccaagcaa ttcctcttcg
     5101 gtcatccgtt atctccgtgt caacgattcg tctgcgcaga ttgctcgcaa tagcccggtc
     5161 agcagggcgg tccgcggcac gatgctgctt acttcgatgt actcgtccgg gctatggtcc
     5221 aacccgccga tcggcccgag accgtccagt cccgccgcgc cggcatggac cgcgatcgag
     5281 acgtccgagc cgccgccggt cgaagcgccc ttgagcgaga agccgagctg gccggcaatc
     5341 cggatcgcca actcctcgag gcgctgcgtg ccgggtgtcc gctccatggc cggcatgccc
     5401 gcaccgccgt tgagcgacat ggtttgcgtc acgcccggga cccattcggt gtcgacgata
     5461 gcctgcagcg cgccggccag ctcctccagc tcctggttgg tccaggcgcg gatgtcgacc
     5521 agcaccgatg cctcggccgc gacaatgttc gggttcgcgc cgccagcgat cttgcccggg
     5581 ttgagcgtca tcccgggcgt gcgcccgttg agcttgaacg actcgacgat cacgttcgcc
     5641 agggcgagcg tcgcgctcgc gcccttctcc ggctcgacgc cggcgtgggc cgccttcccc
     5701 ttggcatcaa tccggaacca acgggttgcc ttgcgcgctg tgacgatgtc gccgttcgcg
     5761 cgggccgctt cgagggtcag gacgacgtcg tgcgcggcgc cttccgatcg cagcagctca
     5821 accgaatggc gctgctcgat ctcctcgtcg gacacgatca ggatcgtcag cagctcgtag
     5881 tcggtccaac cgatcgcgtt cagcgcctcg accgcgtaga tcccggtcag gattccggcc
     5941 ttcatgtcgc acgtgccggg accgagcaga tggtcgcctt cgatgacgac cggtcgccgc
     6001 gccgcttctc cgaccgggta gacggtatcg gcgtgaccga tcatcaggat ccgccccttg
     6061 ccggttccgc gtcgccgggc aacgaggtca tcgccccact cggcattgcg gacacgctcg
     6121 acatcgaagc cgagctcggc cagccggcgc tggaagtgat cctgcacttg atcaatgccg
     6181 accttgtgat aggagccgga gtcgatcgcg caaacccggg tcagttccgg gatatagcga
     6241 tcccgcgcga tctgaagata tgtcgtgatc cgactgtcga acgacattgc ctaccagccc
     6301 gcgtacgcgg cgaccgcggc ttcgtcgact cgcccgttga ccgcgtcgtc aatcgcctgg
     6361 tcgaggatcg ccacaccctc atcgacttcg gcttccgtca ggatcaatgg cggcgtgatc
     6421 tcgaggacgt tggaatatgt gccggcgtag aagacgatca agccaagttg ccaagcgcga
     6481 tacacaaccc gcgccgccaa cgcagaatcg ggcttcttgc tttcacgatc cgtgaccaga
     6541 tcgaccccgc agatcatgcc gcgaccacgg acatcgccga cctggtcatg atgcatcagc
     6601 gtgtcgaggc gtcgcagcag gtatgcgcca acgcggtccg cctgctcgac caggccttcc
     6661 cgctcgacga cgtcaagtcc ggccagggca gcggcacagc tcgtcgcgtt gcccgagatc
     6721 gtgtacaagg ccagcccggt gccgatatcc agcacttcag gcgggccaat aatggcgctc
     6781 aacggcagcc cggccccgag tgccttgccg agcaggacga tgtccggctc aataccggca
     6841 tgctcgtatg agaacatcct gccggtccgg ccgagaccga tcttgacatc gtcaatcacc
     6901 agatagatgc cgtagcggtc gcagagcgag cgcagcttgg gcagaaaatc gtccggcggc
     6961 acaatgtctc cgctgtcggc ctggacacct tcgacgaaga tcccggccac gtcatccggc
     7021 gggcagatgg tggcgaagag cacgtcctcg aggtaactga ggcattggtc cgtcagtgat
     7081 tgatccgggt gcggcaggaa tggccggcga tacggatccg gatacgggat cttgacgata
     7141 ttcgagccgc cgaagcgatc gttgaatccg gcaaacccgg aaagcgccat ggtcgcatcg
     7201 gtcgtcccgt gccagctccc gatgaacgag atcagccgtc gcttgcccgt cgccttgagc
     7261 agcagccgct gggccgcctc gctggcgtcc gacccggaca gaccgaacca gaccttcttc
     7321 ggctgccgcg acatcatccc ggccaaccgt tcggccagca gaccgcccgg ttcgttgaat
     7381 gccgaggcaa gcaccgagca ttgcgtccgg tcgatctggt ccttgatcgc ctggcgaacg
     7441 gtcttgtcgc tgtacccgag atttgcgaga gaccagaggg agctgaagtc gaggaaggag
     7501 ttgccctcgg catcgaacag ccgcgttccc tctccacccg ccaggacaaa cggcgtatag
     7561 cggatcttga tcgcatcggc aacgaactgg ttgtcgcgct cgatgatcgc ggatgatgtg
     7621 accggctctg ctctgaccat ggccgcccct tcctgcgcgc agggaaacat gccctcgcct
     7681 gtcgcgaggg catgttcgca tacctgagct ggttacgcct tcggcaccgt cttcggcaca
     7741 gtcgtcttga tcagctcgaa cttaccgtcc ttggcctcca ccagcgcgac cgatttggtc
     7801 gggatgtggg aatccttggt gtagctgatc tcgccggtcg cgcctttgaa cccggtggtc
     7861 gcggcgagcg cgtcccggat cttgtcgccg gccgtgtcgt tggctcgcgt gatgccatcg
     7921 gccagcaggt tgatgccgtc gtagccgagc gccgcgaaga tcgagtccgg cgccttgccg
     7981 tattccttgt tgtactcgtc gttgaacttc ttggccgcgt ccgactggtc gccgtagatg
     8041 ccctcgtgcg tggtgaagaa gacattgcgg gcattcgcgc cggccagctc gaggagctgc
     8101 ggggtgtcgt agccgtcgcc gccgaggatc ggtgtctcga taccgccctc gcggacctgc
     8161 ttgacgatcg tgccgatctc ctccggtccg gcggagatca tcagcacgtc aggtgccgga
     8221 ttcagcccct tgatctcggt gacctggctg gagaagtcag tgtcgccgac cttgtaggtc
     8281 agctccttga tcacctggcc accgatgtca tccatcgtgt agcgctcctt gaagtactgg
     8341 gccaggaact tggtgtaggt catgccgtcg ttgacgagca tggcggccgt cttccagccc
     8401 ttctcctgcg cgaactcagc ctcgaccgca gcctggacgt tgtcaccgaa gggcagcatg
     8461 aacatgaaat cgccgatctg ggcgatgatc ggggctgtgc caccgacgtc gatgaacggg
     8521 atgcctttct cctgggccac ctgaccgccc gcgcggacat agtcggtatc ggtcagaccc
     8581 acgagcgcga tcaccttgtc ctcatcgacc atcttcttgc agatgttcga gaccgtggtt
     8641 acgtcgctct cgccgttctc aaccacgagc ttcagttgct tgccgagcac gccaccagag
     8701 gcgttcaaca gtttggcggc cagcagcgat ccgtccttgg ccgggacgtc gatcgactgc
     8761 agcgagccag tcaggttgta cgggcagccg accacgatct cgccggcatc ctgcgcgaag
     8821 acggtcgtcg tctcactgac cttgatcatc gcggccagca tcggcgcggc tactccaagc
     8881 tcggcgccac ggcggagaat gtcgcgacga ttgacccggc gatcgattga atcgtggatc
     8941 aaccgggaca cggaatcgct gcgttcactg ttgctcacgc gttactcctc tcaccgggca
     9001 atcccggctc agattccggc ggcaaatatg cgccgactac cgtagtcggc tgtgccgcaa
     9061 gatcatccag tgcgtttgga ttctactgcg ctctatctga ccccgcctgc cgctttccga
     9121 gccatccggc gaggtcgatc tcccggctac ccatcaagcc actcggccgc aggatcatca
     9181 cggcgatgaa gatcagggcg aggatgatct gcgaaagacc gaatgcctgg tcccagtgga
     9241 tcggtccgat cgtgacgttg gtctccagat tgcgaagcac ctccggtgcc agcgtcatga
     9301 agatcgcacc gaagatggcc ccggtgatgc tgcccaggcc gccgaggacg accatgatga
     9361 tgatgttgaa cgttgtcgtg aagtagaagg aattcggcga aacggtgccg atctggtgac
     9421 cgagcaggca tccccccacg ccggtcagga aaccgcatac ggtgaaggcc agtaatcgcg
     9481 cccggtgaac acgcaccccg accgcccggg ccgcgataag gttgtcgcgc gaggcgatca
     9541 tcgaccggcc gaaactcgac cgccgaatcc gccaggccac gtagatcgtc agcaccgccc
     9601 agaaatacgc cgaccagacg ttggtatagg tcggaatcgg attgagtccc tgcgaaccaa
     9661 aggtgaccga gtcccactgg atcaggacgg tgtagacgat gatcagaaag cccatcgtgg
     9721 caaccgccac gtaattgcct gagagccgca tcaacggata gccgacgacg accgcgacga
     9781 tcatcgcgat caccccgccg acgatgcagg caaacgcaag tgcgagccaa tccggccatc
     9841 cacttgtgtc gaactctgcc agccagctcg gcaggcccgg cagcaggatc ggatccttcc
     9901 aggtcagcgg caggctgatc agggcaccga cgtaggctcc gatcgccatg aagccgacct
     9961 gaccgaggga aaagactcct gtgaagccgc ttgacatggt caggctgacc gcgaccacga
    10021 tgttgatcgc gaccagcacg acgagctgga tccggtaact ggtgaaggtg tcctgggcaa
    10081 tccagaggaa gcccgccaga acgagaagtg ccgctccgta aactgccgcc ctggccatct
    10141 agatcttctc cggctcggtc gaaccgagaa tgccctgcgg ccggaccagg aggatcacga
    10201 tcaatgccgc gaagatgaac gcgtcccgat agcccgtcag atgatccggc aggagcgcga
    10261 ccaggaagat ctcgccgaag ccgagaatga atgcgccgag caccgcacca ggaatcgagc
    10321 cgaatccccc gatcacggtc gcgacgaagg ccttcaggac cgggacgaag cccatcaacg
    10381 gctcaattcg cccaacgcgc gcgccccaga gcacgccggc cactcctgcc agcgccgaac
    10441 cgatgatgaa cgccgacacg atcaccgtgt tcgtattgat gcccatcaac cgtgccgcga
    10501 ccgggttctc ggccgttgcc cgcatctgga taccgagccg agtccgcgtc acgaaccagg
    10561 tcagaagcgc gagcaggatc aaggcgaccg cgatagtgat gatgttggtg acgctgatcc
    10621 gcactccccc gcctattccg acccgctcct cgaaactgcc cggcgtcggg aacttcttcg
    10681 gttggacgcc aaagatcatc gaagcagagt tgcgcaccgc aatcgagacg cccagagagg
    10741 tcagcaacag cgaaatctcc ggcgcgccgc gcagcggccg gtacgccacc cgttccatca
    10801 gcaaaccggc gatgatcgtg ccgcccacgc cgatgatcgc ggccggcagg aacggaaatc
    10861 cggctcgctc ggcgtaatag gtgaagaacg cgcccagcat caacacttcg ccgtgcgcga
    10921 aattgatcaa ccgcaggatc gagaagacca tcgccaggcc gatcgcaacc agggcgtaaa
    10981 tgctgcccag gctcagcgca ttgatgaatt gctggccgaa ctccgttccg ctcatgcgcg
    11041 cgctccgcca ccgaggtacg cctgctctac cgccgaattc cgggccaact cggccgcggc
    11101 tccgctcaga ctgatcaagc cggtctcgag cacataggcg cgatccgcga ccgccaatgc
    11161 cttccgcgcg ttctgctcga cgagcaggat cgtcacgccg gtctccttga gctggcggat
    11221 gacgtcgaag atccggttca cgatcagcgg cgcgagacca agcgacggct catccagcaa
    11281 caggagcttc ggcttgccca tcagggctcg cccgatcgcc agcatctgct gctcgccgcc
    11341 ggacagcgtg cccgcccgtt gcgatatccg ctcacccagg atcggaaaca gatcgaagac
    11401 gcgctgaaga tcggcatcga acccgtcccg atccgacctc gcgctcgccc cgagcatcag
    11461 gttctctcgc acggtcagcg atccgaagat ctgccgccct tcaggcgcct ggacgatccc
    11521 gctggagacg atctgatgcg tcggcatccg cgagagatca cgcccctggt aggtgatcga
    11581 cccgccgctc ggccggacga tgcccgagac ggtcttgagc gtggtcgatt tgccggcgcc
    11641 gttggagccg atcagcgcga ccaactcccg ctcctcgacc tcaaatgtga tgcccttgac
    11701 cgcctggatc gcgccgtaat tgacgcggag gttgtccacc ttcagcatca gccgacctcc
    11761 ccgagcggtg caggctcctc ctcggcggcg ccgaggtatg cctcgattac agccgggttg
    11821 gtctgaatct cggtcggtgt cccctccgcg atgatcgagc catagttgag gacctggatc
    11881 cggtcgcaga ggcgcatgat cagcgacata tcgtgctcga cgacgatgat cgtcaggtcg
    11941 aacttctccc ggatctcgcg aatgaagccg gtcaactccg ccgtctcctg ctcgttcatg
    12001 ccggcggccg gctcatcgag caacaggatg gccggactgg tggcaatggc ccgggcgatc
    12061 tcgagcttcc gttgcattcc atacggaagc gcggtcgccg gctggtcggc atagcggtcc
    12121 atgcccacct cgcgcaggtg actgatcgcc gcttcgcgca tgcggcgctc gttccccagg
    12181 aaaccgggta gggagaggag cgtctgaaac agccccatcg gctgccggac ctgttgagcc
    12241 gtcaggacgt tctcgagaac cgtcatgccc gagaagaggc ggatattctg gaacgtgcgg
    12301 gcgatgccgg cgcgcaggat cgcatccggc gccttgccga tgatcgaaat gccgttgagg
    12361 tcgatctggc cgtcggtcgg cggcacgaat ccagtgatga cgttgaagag cgtcgtcttc
    12421 ccggccccat tcggaccgat cacgccgagg atttcaccct tgcgcagcga tacggagtag
    12481 ccggagagcg ccttcaggcc ccggaacgac cgcccgacat ccgccgcctg caggacgacc
    12541 ggattggcgt cggcgatctg atggtcgacc atatgcgggc gggccctcgg gtgcatcatt
    12601 cagcaggtat tcgaacgctg ggtaagggta ccgcagcatc tgtacatgcg cgagagggtg
    12661 agatttgatg cgacgactgc tcctgctgct cctgatcgtc atgacgctag gtgcaatctc
    12721 ggccgcggac gcggtcgccg ctcccgcctc ggcgacacca gaaacggagc ggtgcacggt
    12781 cgatccgctc tcgccggaac acttgactgc gatcgttgcg gccggattcg agccgacccc
    12841 aaggctggag aaaggcgatc aagcggtccc ggcgacggac ctggtcgcca tgttcgacgt
    12901 cctgaccgag agcgtagcct gcaccaatgc gaatcaaccg atgcgggcgc tggccctgta
    12961 taccgatcgc taccttgcgg agcgcttttc cgggcaagcg ggcgaagacg agctcgggca
    13021 cctgattgcc gcatcatccc gctcgccgaa gcctgccgca cccggagatc agctcgtgct
    13081 gttggccgtc gcggatccca tcctctacgg cgacgggagg atcggcgcga ccgtgacgac
    13141 cgcgaatgcg gaagcaacct tcattgatca gatcatcttc gccaaaacag aagaggggtg
    13201 gcgaatcgat caggtcgtgc tcggcaagga gcagcacgcg acgcctgccg cgacccccgg
    13261 tagcacgcag ccctgattcc ccgtaggatg accgcatcag gaggataggc gagtcatgcc
    13321 caagtcacac gtcgaccggt caacgacggc cccgcatccg gagccaacca tagaggtccc
    13381 cgcggacgag ctcgccgcgc tcaaggagat cgagcgccga atcctttggc tctcgaccct
    13441 gatgatccac cacgccaata acgtacggcc cagcctcgag aaatcgaagg tcggcggtca
    13501 ccaggcctcg tcggcatcgg tcgcctcgat catcaccgcg ctctacttcc atttcttacg
    13561 cgggggagac cgggtctcga tcaagccgca cgcgtcgccg atctaccacg ctgcccacta
    13621 cctgctcggc aacctcgacc gcaagtacct gacgaccctg cgggagtaca agggattgca
    13681 ggcctatccc agccgcacca aagacccgga cccggtcgac ttctcgaccg gttcagtcgg
    13741 cctcggcgcc gtcgcgccgg tcttcgccgc gcttgcccac aagtacgccg agctgcactt
    13801 cggagaggtg acatcgaacc ggttcatctc gctgatcggc gatgccgagc ttgacgaggg
    13861 caatgtctgg gaaacggtcg ccgaagaggc aattcgtggt ctcggcaacg tgatctggat
    13921 cgtcgacctc aaccggcaat cgctggatcg cgtgatcccg ggcgtccggg cgggcaggct
    13981 caaggcgctc ttcgccggcg ccggttggcg cgtcttcgag acgaagtacg ggtcgacgct
    14041 gcaggcggcg atggacacct cgccgaacga cgcgctccgc cgccgcatcg acgagatgag
    14101 caacgaggag taccaggccc tcattcgact gcgggatggc gccgagatgc gccgccggct
    14161 ggccgacgtg ccgcagagtg cagacagcaa ggacatcctt ctctcggttg ccgggattcc
    14221 tgacgagggc cttggccacg ttatctccaa tctggccggc cacgacctgc cgcgcttact
    14281 ggag
//
LOCUS       DATAOZ010000079         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4768642, whole genome shotgun sequence.
ACCESSION   DATAOZ010000079 DATAOZ010000000
VERSION     DATAOZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4768642"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VHR64_02300"
     CDS             <1..727
                     /locus_tag="VHR64_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3301683.1"
                     /translation="CDTMNAPTSDIRVRQALSMVIERSTLTDQILAGAFTAAQTVLPP
                     DIPGHNPAAALGEDVHRAQQLLAEAGFPGGEGFPDISLVYHTADLAERTTAEYLQEAW
                     NRNLGISVELDPMEEAAYQDWFQSRASQPFNLYIYQFGSDWSDPANWHNQLFDSRSDF
                     YHTHWKDDEFDRLVREAAGVTNDAARIAQYQQAEAILVRDAPFIPLYNLNRVYVIKPN
                     IRGVYHNPILGRTWIKYISVVEG"
     gene            complement(857..932)
                     /locus_tag="VHR64_02305"
     tRNA            complement(857..932)
                     /locus_tag="VHR64_02305"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(897..899),aa:Lys,seq:ctt)
     gene            complement(985..2445)
                     /gene="gatA"
                     /locus_tag="VHR64_02310"
     CDS             complement(985..2445)
                     /gene="gatA"
                     /locus_tag="VHR64_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX3301684.1"
                     /translation="MAISDLVWASAREQRELLDSGQVSAVELTRAHLDRMDAVEPALS
                     CHLHRMDEIALAQAEAADQRIRSGDAQPMTGITVSLKDVLCTTDAPTTAASRILEGYM
                     SPYDATVVERLRLQGAVFVGKSNTDEFAMGSSTENSAFGPTRNPWNTDFVPGGSSGGP
                     SAAVAAAEVAIGLGSDTGGSIRQPAGFCGVVGLKPTYGRVSRYGLIAFASSLDQIGPF
                     TRTVEDAAIALSAIAGHDPRDSTSSPRPVPDFRAALSGDLRGMRIGVAHEYSVDGMEP
                     GVEAAVAAAVEQIKQLGAEVVEVSLPHTSYALATYYITAPAEASANLARFDGVRYGLS
                     CDAGSLKAMYETTRGSGFGDEVKRRIMLGTYALSSGYYDAYYVKAQKVRTLIKQDFDQ
                     AFASVDAIVAPTSPTVAFRLGARTDDPVQMYLADVFTIPANMAGIPGVAVPCGFSDGL
                     PVSLQVLGRSFDEQTILRVAHAYEQSTEWHKHRPPV"
     gene            complement(2448..>2602)
                     /locus_tag="VHR64_02315"
     CDS             complement(2448..>2602)
                     /locus_tag="VHR64_02315"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX3301685.1"
                     /translation="PPTAQTIELADVMRDDVAGPSLPLEQVLMNAPRQRDGFFEVDTP
                     FGGEEG"
BASE COUNT          475 a          842 c          830 g          455 t
ORIGIN      
        1 ctgcgacacc atgaacgcgc cgacatcaga catccgggtc aggcaggcgc tttccatggt
       61 gatcgagcga tcaacattga cagaccagat cctggccggc gcgttcacag ccgcgcagac
      121 ggtgctgcca cccgacatcc ccgggcacaa ccctgccgcg gctctcggtg aggatgtcca
      181 ccgggcccag cagctcctgg ctgaggccgg attccctggg ggcgagggtt tccctgacat
      241 ttcactcgta taccacacgg ctgacctcgc ggagcgaacg accgcagagt atttgcagga
      301 ggcgtggaac cggaacctgg ggatcagtgt cgagctcgat ccgatggaag aagcggcgta
      361 ccaggactgg ttccagtccc gcgcaagtca gccgttcaac ctgtacatct accagttcgg
      421 ctcggactgg tctgacccgg ccaattggca caaccagctc ttcgattccc gatccgactt
      481 ctaccatacc cactggaagg atgatgagtt cgaccggttg gtgcgtgaag cagctggagt
      541 gacgaacgac gctgcccgaa tcgcgcagta ccagcaggcg gaggcgatcc tcgtgaggga
      601 tgccccgttc atcccgctct acaacctgaa tcgggtttac gtcatcaagc ccaacatccg
      661 gggcgtgtac cacaacccca tcctcggccg gacctggatc aagtacatct cagtcgtcga
      721 gggatagcga ctcgaccttg tggtccatgc gcccggtgcc acgggcaccc cggactcctg
      781 cgggacgact ggcccttgaa tgcacgaaac cggagcgtga gttcgccccg gttcctctac
      841 tggatggcgc tttggctggt gagccgtggg ggactcgaac cctcaaccca ctgattaaga
      901 gtcagttgct ctgccaattg agctaacggc ccggacgaag tttaccgggc cgccgccatg
      961 ttgacaagtt tcggaagaaa tccgtcagac cggtgggcgg tgcttgtgcc actcggtgga
     1021 ttgctcgtag gcgtgggcga cgcgcagaat ggtctgctca tcgaacgatc ggccgagcac
     1081 ttgcaggctg accggcaggc catcgctgaa gccgcacggg accgctacgc cggggatccc
     1141 ggccatgttc gccgggatgg tgaagacatc ggcgaggtac atctgcaccg gatcgtccgt
     1201 gcgcgcgccg agtcggaacg cgacagtcgg ggaggtcggc gcgacgattg catcgaccga
     1261 cgcgaatgcc tgatcgaagt cctgcttgat cagcgtccgc actttctgcg ccttgacgta
     1321 atacgcgtcg tagtagccgc tgctgagcgc gtaggtgccg agcatgattc gccgcttgac
     1381 ctcgtcaccg aagccgctgc cccgcgtcgt ctcgtacatc gccttgagtg agccggcgtc
     1441 gcaggaaagg ccataccgga cgccatcgaa tcgcgcgagg ttggcgctgg cttccgccgg
     1501 agcggtaatg tagtaggtcg ccagagcgta gctcgtgtgc ggcagcgaaa cctcgacgac
     1561 ttcggcgccc agctgcttga tctgctcgac tgccgctgcc accgcagctt ccacgcccgg
     1621 ctccatgccg tcgaccgagt attcgtgggc gacgccgatc cgcattccgc gcaggtctcc
     1681 ggacaaggca gcccggaagt caggtacagg tcggggcgag ctcgtcgaat cgcggggatc
     1741 gtgcccggcg atcgctgaga gcgcgattgc ggcatcctcg actgtccgcg tgaacgggcc
     1801 gatctggtcg aggctgctag cgaaggcgat cagaccgtac cgggaaactc ggccgtacgt
     1861 cggcttgagt ccgaccacgc cgcagaatcc cgccggttgc cggatcgagc cgccggtatc
     1921 cgatccgagt ccaatggcca cctcagccgc cgcaaccgcg gccgaaggtc cgcccgagct
     1981 cccgcccggc acgaaatccg tattccacgg gttgcgcgtt ggcccgaacg ccgagttttc
     2041 ggtcgacgat cccatcgcga actcatcggt attgctcttg ccgacgaaca cggcgccctg
     2101 cagccgaagc cgctcgacga cggtcgcgtc atacggcgac atgtagcctt cgaggatgcg
     2161 cgaggcagcg gtcgtcgggg cgtcggtggt gcaaagcacg tccttgagcg agaccgtgat
     2221 gccggtcatc ggctgcgcgt ccccggacct gatccgctga tccgcggcct cggcctgggc
     2281 aagcgcgatc tcgtccatgc gatgcaggtg gcaggaaagt gccggctcga cggcatccat
     2341 gcgatcgagg tgcgcgcggg tcagctcaac agctgatacc tggccagagt ccagtaactc
     2401 gcgctgctcg cgggccgacg cccagacgag gtcggagatc gccacgatca gccctcctcg
     2461 ccgccgaacg gcgtgtcgac ttcaaagaac ccatcgcgct gccggggcgc attcatgaga
     2521 acttgctcga gcggcagcga tgggccggca acgtcgtcgc gcatgacatc ggctaattcg
     2581 atcgtctgcg cggttggcgg aa
//
LOCUS       DATAOZ010000080         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1876121, whole genome shotgun sequence.
ACCESSION   DATAOZ010000080 DATAOZ010000000
VERSION     DATAOZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876121"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VHR64_02320"
     CDS             <1..762
                     /locus_tag="VHR64_02320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX3301686.1"
                     /translation="NWIADHLDDSAVRIIALTPAEDFAKGHIPGAAQVDWPDLNLTDS
                     ADATVEKWRGDIEKRLTDLGVMPDSTVVIYDGGTFYGARLWWILDQLSHKDKRIIDGG
                     LGAWTKSGGKIETGAANPTSASQPYHGTPNEATLARLEEVQKASETGAAALVDARNAE
                     EYANGHIPGAVNIPFLDNAVPNSGGTWKSPTDLRAMYAAKGVTPDRQVIPYCSTGVRS
                     ANTWFTLRALGYEQVKLYSASFAEWSADPNRPVDK"
     gene            complement(894..>1702)
                     /locus_tag="VHR64_02325"
     CDS             complement(894..>1702)
                     /locus_tag="VHR64_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301687.1"
                     /translation="IERDGAIDKATLETDEPAPFKVVGNTRGKAISLRIKLSNNEALA
                     LTGVGERDIKSCQGKISGTMAGPEFGDIGVFEIVRRVQPGGTATPAASATATAGGGGT
                     IQPTPGGGGGGGNPTPAPASTSTPCPPQTCSGSKVWDAQQCQCVCYAGGVDCGGDFCC
                     PETMICTGNGECGCSPGKIKCNAACHDACPPGQTFDDSTCTCVDSLCPPGQKECNGIC
                     KDVTNDKNNCGSCGNICQTGVPCIAGTCTCPATMKYCASKQMCIDENATC"
BASE COUNT          306 a          571 c          524 g          301 t
ORIGIN      
        1 aactggatcg ccgatcatct cgatgactca gcggtccgga tcatcgccct gaccccggcg
       61 gaggacttcg cgaagggcca catccccggc gcggcccagg tcgactggcc ggatctcaac
      121 ctgaccgact cggccgatgc cacggtcgag aaatggcgcg gagacatcga gaagcgtttg
      181 accgacctcg gcgtgatgcc tgacagcacg gtcgtcatct acgacggtgg caccttctac
      241 ggggcgcgcc tctggtggat cctcgatcag ctcagccaca aggacaagcg catcatcgac
      301 ggcggactcg gtgcctggac caaatccggt ggcaagatcg agaccggcgc ggccaacccg
      361 acctcggcat cccagccgta tcacggtacg ccgaacgagg cgaccctggc ccggctcgaa
      421 gaggtgcaga aggcgtctga aactggcgct gccgcgctgg tcgatgcccg gaacgccgag
      481 gagtacgcca acggccacat ccctggcgcg gtcaacatcc ccttcctcga caacgccgtc
      541 ccgaacagcg gcggcacctg gaaatcgccg accgacctcc gcgccatgta cgccgccaag
      601 ggggtcacgc cggatcgcca ggtcatcccg tattgcagta ccggcgtccg ctctgccaac
      661 acctggttca ccctgcgcgc actcggatac gagcaggtca aactctactc agcctcattc
      721 gccgaatgga gcgcggaccc gaaccgcccg gtcgataaat aaagcgagca tacggacgcc
      781 tcttctccct ctcccgtgaa cgggagaagg gagatcgttc aggtggagcg cgcaaagtaa
      841 aaccctccca attggttcgc caatcgcgag ggttttaccg tcggatcagg ctgctagcag
      901 gtcgcgttct cgtcgatgca catctgcttg ctggcgcagt acttcatcgt cgctgggcag
      961 gtacaggtgc cggcgataca tggaacgccg gtctggcaga tgtttccgca cgagccgcaa
     1021 ttgttcttgt cgttcgtgac gtccttgcag atgccgttgc actccttctg acccggcggg
     1081 cagagcgaat cgacgcacgt gcacgtagag tcgtcgaacg tctggccagg cgggcaggcg
     1141 tcgtggcagg cagcattgca cttgatcttc cctggcgagc agccgcactc cccgttaccg
     1201 gtgcagatca tggtttccgg acagcaaaag tccccaccgc agtcgacgcc gcctgcgtaa
     1261 cagacgcact ggcattgttg tgcgtcccag actttggagc cgctacaggt ttgcggggga
     1321 cacggggtcg aggtcgaagc cggcgcgggc gttggattgc cacctccgcc tccaccgccc
     1381 ggcgtcggct ggatagtgcc gccaccgcca gcagttgcag tcgcggatgc ggcgggagtg
     1441 gccgtgccgc ccggctgcac gcgccggacg atctcgaaga cgccgatatc gccgaattcc
     1501 gggccggcca tagtgccgct gatcttgccc tggcagctct tgatgtcgcg ctcaccgact
     1561 cccgtcaatg ccagcgcctc attgttgctg agcttgatgc ggagactgat cgccttgcca
     1621 cgcgtattgc cgaccacctt gaatggcgcc ggctcgtcgg tctccagggt ggccttgtcg
     1681 atcgcgccgt cccgctcgat cg
//
LOCUS       DATAOZ010000081         3138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8190304, whole genome shotgun sequence.
ACCESSION   DATAOZ010000081 DATAOZ010000000
VERSION     DATAOZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8190304"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1034
                     /locus_tag="VHR64_02330"
     CDS             324..1034
                     /locus_tag="VHR64_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3301688.1"
                     /translation="MLALQMNRGLGADVAERLRSAILTGYFGPGERLPEAQLAQAIGV
                     SRAPVREALATLEREGLIVIRRNRGAFVAQLSREDLDEVYSLRAAIERLAVERCVAHA
                     TAEDIAQLQGVVDRIAALMERGITEQEAAALDLEFHDIVYRSGRHRRLYDTWATLRPQ
                     IHVLLLNRNVAHEDFRDLVVSSHQEIVDALRSRDLERSVEITMAHLLGSYERVRQSHA
                     PRYGEDSRSAGKANGGQT"
     gene            1031..1846
                     /locus_tag="VHR64_02335"
     CDS             1031..1846
                     /locus_tag="VHR64_02335"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX3301689.1"
                     /translation="MIGSLFELTGRVAVVTGGGLGIGRGIAIGLAQAGADLVICDLND
                     EDMAETARRIEATGRSCRTIRADVGDPAGVDALFALVDREFGKIDILVNNVGIGARHR
                     PEELPIDEFRKVLDVGLYGSFTCAQEAGRRMIAAGTGGSIINISSIAGAAALGRGNLA
                     HSVNKGAINSMTRELAVEWAKHGIRVNAILPCQVLTEGFQKWLDSTSFDPALMDRFLA
                     GIPMNRLATAEDMAGPAVFLASDAAAMVTGTLLGVDGGNLALNAGGSHTWHTA"
     gene            1999..>3138
                     /locus_tag="VHR64_02340"
     CDS             1999..>3138
                     /locus_tag="VHR64_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3301690.1"
                     /translation="MTDQSEKKSRTSNISRRTVLKGAAAAGAAAVAVHPKANPAFAVN
                     YLQGTPITVKYGTWFWNEPGRGDAWKKQVDRFNAAQKDIKIEGVGVPFDQYTNDIIVQ
                     LQAGKIDYDVIQTTPDLVLRLLQADILAPLTKVLEANNITKLSDGHNNLYVNDEVRGL
                     DVVTVIFGLFYNQSLFDTAGVTALPTTVEEWKTVSTQLTKRPNQFGMYSPHNMAEPES
                     FWFTLQEWALPFSGRWATGKTPNLTSEPIMSAVQLFKDMYDSSFPQGVDDATATRQWG
                     EGQIAQELIVSALANVFKDTAPDLYPDLRSMPLPWESKESIARIHPITVNNQKDQTVQ
                     DAAIEWLTDLYKVENYRQLLMDCLDVIPAYDVGDLSEYYASLPWTG"
BASE COUNT          610 a          998 c          995 g          535 t
ORIGIN      
        1 gtaggggcgc ttggcgacaa gcacgatcgt catgttgacc gccaaaacca gcttcatatg
       61 gggacgaccg tgcgtcatgt ccagcctccc cgtttccccg tgcggcgaaa ctgagacacc
      121 cataatcctg ggtccttgcc gcgaggtctc cgagcccagc tcgcgtgaga cgatccaggg
      181 agtcccttga cagccgcaac gcatattcag tagattgtcg acattcgaca atagagtgaa
      241 cgctacacag ctccgggttc agaagcaggg gcccatggag tcgcgaggga tcggatctgg
      301 aaacgtccgc tgaggtaccg aatgtgcttg cgctgcagat gaaccgtggg ctcggcgcgg
      361 atgtcgccga gcgcctgcgc agcgctatcc tcaccggcta tttcggcccc ggcgaacgcc
      421 tgcctgaggc gcagctcgcg caggcgattg gggtcagccg tgctccagtg cgcgaggcgt
      481 tggccaccct ggagcgcgag gggctgatcg tcattcggcg caaccggggt gcgttcgtcg
      541 ctcaactctc ccgtgaggac ctcgacgaag tctattcact acgggcggcc atcgagcggc
      601 tggccgtcga gcggtgcgtg gcccacgcca ccgcggagga tatcgcccaa ctgcaggggg
      661 tggtcgaccg cattgccgcg ctgatggagc ggggcatcac cgagcaggag gcggctgcgc
      721 tcgatttgga gttccacgat atcgtttacc gctccggtcg gcatcgccgc ctctacgaca
      781 cgtgggccac gctgcggccc cagatccacg tgctgctcct gaatcgcaac gttgcccatg
      841 aggacttccg cgatctcgtc gtctcgtctc accaggagat cgtcgatgcg ctccgcagcc
      901 gtgatctcga aaggtcggtc gaaatcacaa tggcccacct gctcggctcc tacgaacggg
      961 tgcgacagag ccatgctcct cggtacggcg aggacagccg gtcggccggc aaggcaaacg
     1021 gaggacaaac gtgatcggct cgttgtttga gctgaccggc cgagtcgccg tcgttaccgg
     1081 cggcgggctc ggaatcggcc gtggcatcgc gatcgggctt gcccaggcgg gagccgacct
     1141 cgtcatctgc gaccttaacg acgaggacat ggcggagaca gcgcgccgaa tcgaggcaac
     1201 cggccgcagc tgtcgcacta tccgtgccga cgtgggcgat ccggccggcg tcgatgccct
     1261 gttcgctctc gtcgaccgcg aattcggaaa gatcgacatc ctggtcaaca acgtcggcat
     1321 cggtgcacgc caccgccctg aggagttgcc catcgacgag tttcggaagg tgctcgatgt
     1381 cgggctctac gggtcgttca cgtgtgcgca ggaagcgggc cgccggatga tcgcggccgg
     1441 caccggcggc agcatcatca atatcagctc gattgccggc gcggccgcgc tcggacgcgg
     1501 caacctcgcg cacagcgtca acaagggtgc gatcaactcc atgactcggg aactggccgt
     1561 cgagtgggcg aaacatggta ttcgcgtcaa tgcgatcctc ccgtgccagg tactgaccga
     1621 ggggttccag aagtggctcg attcgacgag ctttgaccca gcgctaatgg atcgattcct
     1681 ggccgggatt ccgatgaacc ggctggccac cgctgaggac atggccgggc cggctgtctt
     1741 cctcgcctcc gatgcggcgg caatggtgac cggcacgctg ctcggtgtcg atggcggcaa
     1801 tctggcgttg aacgcgggcg ggagccacac atggcacacc gcttagatcg aacctgggac
     1861 gcggctgtat acggaggacg tgagaggagg gatctcgcct gatccagcgg gcgcgttcgc
     1921 cacgtcggga aatcagggcg tggcgttggt tgcagttgtt gcgagtcgat cacaaccagt
     1981 tgtccaggag gacatcagat gactgatcag agcgagaaga agtcacgcac gtcgaacatt
     2041 tcgcgacgca ccgtgctcaa gggcgccgct gcggccggcg cggctgccgt agcggtccac
     2101 ccaaaggcca atccggcgtt cgccgtcaac tatctgcaag gcacgccgat caccgtcaag
     2161 tacgggacct ggttctggaa cgagccggga cgcggtgacg cctggaagaa acaggtcgac
     2221 aggttcaacg ccgctcagaa agacatcaag atcgagggcg tcggcgtccc cttcgaccag
     2281 tacacaaacg acatcatcgt gcagctccag gcgggcaaga tcgactacga cgtcattcag
     2341 acgacgccgg acctggtgct gcgcctgctc caggccgata tcctcgcgcc cctcaccaaa
     2401 gtgctcgagg ccaacaacat caccaagctc tcggatgggc acaataacct ctatgtcaat
     2461 gacgaagtgc gcggcctcga cgtcgtcacc gtcatcttcg ggctcttcta taaccagtcc
     2521 ctgttcgaca ccgccggggt caccgcgctg ccgaccacgg tcgaggaatg gaagaccgtc
     2581 agcacgcagc tgaccaagcg gccgaaccag ttcgggatgt actcgccgca caacatggct
     2641 gagcccgaga gcttctggtt cacgttgcag gaatgggccc ttccgttcag cgggcgatgg
     2701 gccaccggca agaccccgaa cctcacctcg gagccgatca tgtcggcggt ccagctcttc
     2761 aaggacatgt acgactcctc gttcccacag ggcgtcgatg acgcgaccgc cacacgccag
     2821 tggggcgaag gccagatcgc ccaggagttg atcgtctcgg cgctggcaaa cgtgttcaag
     2881 gacaccgcgc cagatctcta cccggacctg cgctcaatgc cactgccatg ggagagcaag
     2941 gagtcgatcg cgcgcatcca cccgatcacg gtcaacaacc agaaggacca gacagtccag
     3001 gacgcggcca tcgagtggct caccgacctc tacaaggtgg agaactaccg ccagctgctg
     3061 atggattgcc tggacgtcat tccggcctac gacgtcgggg atctgagcga gtactacgcg
     3121 tccctgccgt ggaccggt
//
LOCUS       DATAOZ010000082         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1876749, whole genome shotgun sequence.
ACCESSION   DATAOZ010000082 DATAOZ010000000
VERSION     DATAOZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876749"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..2330
                     /locus_tag="VHR64_02345"
     CDS             231..2330
                     /locus_tag="VHR64_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX3301691.1"
                     /translation="MTADEMVPGADRRSQRVRARRRQGSLVQGDLVGKQGMQGRLLGD
                     RYVRVCRVQSGDFERAAPGHLIATEEALEARGPAGRAFGKIKRTVIGAPLSTAQAAHE
                     RLTKTKALAVLSSDALSSVAYATEEILQVLLLAGLGALALSLPIGVGIVALLLIVGVS
                     YRQTIKAYPTGGGSYIVAKDNLGELPALTAGAALLFGYVVTVAVSIAAGVAALTSAVP
                     PLRDHPVILGLGFILLVTVLNLRGIRESGSIFAVPTYLFLIGIIGMILLGLVRAASHG
                     FVPQAPVMPPGEIGAGTQTVGILLILTAFSRGCAALTGVEAISDGVPAFQPPEWKNAR
                     ATLTGMIIILASTFSGITFLANQFGIVPRQPDTPLGYETVVSQIARTIFGGGTPAYYY
                     IQVATLAILILAANTAYSDFPRLSYFLARDRYLPKQFTFRGDRLAYSTGIVTLGILSS
                     LMLAGFGGETTRMIPLYAVGVFTAFTLSQGGMVMRWLRLREPGWRVGLAINVVGVVTT
                     GIVAIVVGLTNFAKGAWIVLLLIPLLIMMFRAIHRHYARAAGELAAQTPLEPDQIEHT
                     VIVPVAAVNRVARQTLAYARSISPNVTAVHITDDEAEIEQMRESWNALGSDIPMVIIE
                     SPYRSLVGPLLSYLDEIDRQRPDDTLTVVLPEFIARHWWEQILHNQTALRIKAALLFR
                     PGTVVTSVPYHLERRGA"
     gene            2445..>2770
                     /locus_tag="VHR64_02350"
     CDS             2445..>2770
                     /locus_tag="VHR64_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3301692.1"
                     /translation="MPPSIDRSHLTVDLPRPLTSFIGREREVATIVDRLRRADVRLVT
                     LTGPGGVGKTRLAIRVAETVAVDFPDGVWFVSLATVRDPALVASTIARTLGVEETGTR
                     SVADGIV"
BASE COUNT          436 a          934 c          875 g          525 t
ORIGIN      
        1 gtctgttcgt cgcgccttcg tcgatgacga tgggcgcagc agcccgcgga gtgccatgaa
       61 tggcgaccgc tcgatttcga tctcatcgtc gtcctaagcc atcagcggcg gacgctgaca
      121 caccattcga cagcagtgaa gatcgcctcg gcgaaagccc gagcgacaat ggcgtaacgc
      181 agcctgcggc gcgaccgttt gtccactccc aagagaacgg cgaagccacg gtgaccgctg
      241 acgagatggt gcccggtgcc gaccgtcgca gccagcgcgt gcgggcacgg cgccggcagg
      301 gatcgctcgt ccagggcgat ctggtcggca agcagggtat gcaggggcgt ctgcttggtg
      361 accgctacgt ccgggtctgc cgggtgcaat cgggcgattt cgaacgtgcg gcgcccggtc
      421 acctcatcgc caccgaagaa gcgctcgaag cgcgcggacc ggccggccgg gccttcggca
      481 agatcaagcg caccgtcatc ggtgctcccc tcagtaccgc gcaggcggct cacgagcgtc
      541 tgaccaaaac taaagcgctg gcggtcctct catccgacgc cctctcatcg gtcgcttacg
      601 ccacggagga gattctgcaa gtccttctcc tggctggcct gggcgcgctc gcgctcagcc
      661 tgcccatcgg cgtgggcatc gtcgccttgc tcttgatcgt cggtgtctcc taccggcaga
      721 cgatcaaggc gtacccgacg ggtggcgggt cctatatcgt cgccaaggac aatctcgggg
      781 agctgccggc cctgaccgct ggcgccgcgc tgctcttcgg ctatgtcgtc accgtagccg
      841 tcagcatcgc cgccggtgtt gccgccctga cctcggcggt accgccgctg cgcgaccacc
      901 cggtgatcct cggcctggga ttcatcctgc tggtgaccgt tctcaaccta cgtgggattc
      961 gtgaatcggg cagcatcttc gccgtgccga cctatctctt cctgatcggc atcatcggca
     1021 tgatcctgct tggactcgtc cgcgcggcat cacatgggtt cgtgccgcag gcccctgtga
     1081 tgccaccggg cgagatcgga gccgggaccc agacggtcgg catcctgctc atcctgacgg
     1141 ctttcagccg gggttgcgcg gcactgaccg gcgtcgaggc gatctcggac ggggtccccg
     1201 cgtttcaacc gccggagtgg aagaacgcgc gtgcgacgtt gaccgggatg atcatcatcc
     1261 tggcctcgac attttcgggg atcaccttcc tggccaacca attcgggatc gtgccccgcc
     1321 aaccggatac tccccttggc tacgagacgg tggtttcgca gatcgcgagg acgatcttcg
     1381 gcggcggtac ccctgcgtac tactacatcc aggttgccac gctggcgatc ttgatcctgg
     1441 ccgcgaatac cgcctactcc gacttcccgc gcctctccta tttcctggcc cgcgaccgct
     1501 acctgccgaa gcagttcacc tttcgcggcg atcggctggc ctactcgact gggatcgtga
     1561 cgctgggcat cctctcttcc ctcatgctgg ccggcttcgg tggcgagacc acgcgcatga
     1621 tcccgctcta cgccgtcggg gtcttcaccg cgttcacgct ctctcaaggg ggcatggtga
     1681 tgcgctggct gcgcttgcgg gagccgggct ggcgggttgg cctcgcgatc aacgtcgtcg
     1741 gcgtggtgac gaccgggatc gtggcgatcg tcgtcgggtt gacgaacttc gccaagggcg
     1801 cctggatcgt cttgctcctg atacctctcc tgatcatgat gttccgcgcc attcaccgcc
     1861 actatgcgcg ggccgccggc gagcttgccg cgcagacccc gctcgaacca gaccagatcg
     1921 agcacaccgt catcgtgccg gtggccgccg tgaaccgcgt ggcgcgccag acgctggcct
     1981 atgcccgctc gatctcaccg aacgtcacag cggtccatat cacggacgat gaagccgaaa
     2041 tcgagcagat gcgggagagt tggaatgcgc tcgggtccga cattccgatg gtcatcatcg
     2101 agtcgcccta tcgcagcctg gtggggccgc tcctgtcgta tctcgacgag atcgaccggc
     2161 aacgtcctga cgacaccctg accgtcgtcc tgcccgagtt catcgcccgc cattggtggg
     2221 agcagatcct gcacaaccag accgccttgc ggatcaaggc ggcgctcctc ttccggcccg
     2281 gcacggtcgt gacgagcgtg ccgtaccacc tggagcggcg aggggcgtag aagtcgaatt
     2341 ggtcaagggg tcaagattcc tgtctcgcgc ggaagattga ccatcgtgcc ccacatctct
     2401 catgatgacg agtaatcgaa cataggatcg ccgcggggcg cgacatgcct ccctcgatcg
     2461 accgctccca cctgaccgtc gacctgcccc gaccgctgac ctcgttcatc ggccgggagc
     2521 gtgaggtcgc gaccatcgtt gaccgcctcc gccgtgcgga tgtccgcctg gtgacgttga
     2581 cgggtccagg cggcgtgggc aagacccgtc tggcaatccg agtggcggaa accgtggcgg
     2641 tcgatttccc ggatggggtc tggtttgtgt ccctggcaac ggtccgtgat cccgcgctgg
     2701 tcgcgtcaac cattgcgcgc acattggggg tcgaggagac agggactcga tcagtcgcgg
     2761 acggcatcgt
//
LOCUS       DATAOZ010000083        15661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6612479, whole genome shotgun sequence.
ACCESSION   DATAOZ010000083 DATAOZ010000000
VERSION     DATAOZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15661
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6612479"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VHR64_02355"
     CDS             complement(<1..1098)
                     /locus_tag="VHR64_02355"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX3301693.1"
                     /translation="MARRVSDTDVAKSSGLSDADIRVAASEILAGHSERSWHARLSPF
                     LGPAFIAAVAYVDPGNFATNIQAGAEFGYLLVWVIVASNLMAMLVQSLSAKLGIATGR
                     NLAEVCRERFPRPVVYGMWIVAEGVAMATDLAEFLGAALGFNLLFDIPLFPAALLTAV
                     ATFLILGLQRHGFRRLEAVITVMVGVIAVCYLLETLIGNPDWGAIAHGAVTPQFDGTE
                     SVLLAVGILGATVMPHVIYLHSALTQGRIPLRSVADARRVFKLERFDIWLALGIAGLV
                     NGAMLVVAAAVFFSSGHTEVTQLDQAYRTLTPLLGQSSSELFGVALLVSGLSSSTVGT
                     LAGQVVMGGFLGWRIPTFVRRAITMAPALIVI"
     gene            1274..2203
                     /locus_tag="VHR64_02360"
     CDS             1274..2203
                     /locus_tag="VHR64_02360"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3301694.1"
                     /translation="MQSDHYDALIIGGGPAGLSAAIYLARSLRSVVVIDSDRPARSDW
                     VQVNHNFLGFPGGVSIIELGQRGREQAEQFGARIINAEVTSLTQADGHFEAVVDSEVV
                     RGRAVILATGVRDRWVEFPGYEEFIGRSMHSCIVCDGYEMRGQRVLIVGNDEHAAIVS
                     IQMCRFTDRITLLIHPDAPPLPKPLQAELARSGIRCVTGEIATARAREPGYFEAVVLA
                     GGEEIELDHLFSEQGAEPNVALAQALDMALTDEGYIKVDTEARTSIPYIYAAGDVTRL
                     FSHQVITAAHEGATAATTLAWDLFAQDRTNRRP"
     gene            complement(2240..3217)
                     /locus_tag="VHR64_02365"
     CDS             complement(2240..3217)
                     /locus_tag="VHR64_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3301695.1"
                     /translation="MRGSEQKAWLDRLTADLPNLRGAMTWGIDHGDGELVLGIAVSLW
                     RYWLARGELREGSVWLERGLAAGHLSISIRCQGLASAASLARLRGDFATAERLGNELL
                     SIARATDDEWGIVTGLSILGLIISCQGRLDEAAQLGEEALTHARDFGDTHILALAHYR
                     LADAYDERDWIRAREHYREALRLWQSIDHGWGICGALLQLGVSEHRLGNPAKAVACYR
                     DELLLALEIDDRSLVAAGLVCLGEAVSLTNQLEPAARLLGTSSALNSAFGGVRNRVAE
                     KQYQSTLARIKEGLGPDAFHAYWQDALSKPIREIVHEALTIADSLQPQA"
     gene            3260..3688
                     /locus_tag="VHR64_02370"
     CDS             3260..3688
                     /locus_tag="VHR64_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301696.1"
                     /translation="MSLANGACLGALIERTFSKLSNGLQQEKPRLSRWAIYLPNKIFV
                     DQNGNLIVQGRRIGVAGHGGHGIQGAPAGKNAEAPKNALMLGGEEGVTPRNRGFDSAL
                     ARGGIRRPLTQYLQMRSNERAKRGWRQYSHPGGGELDCQR"
     gene            4046..4408
                     /locus_tag="VHR64_02375"
     CDS             4046..4408
                     /locus_tag="VHR64_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301697.1"
                     /translation="MIRITRAKGKRNGREHEIRIIEGREIDPDDAAGKLHGHPAGGCK
                     RQSRFSDASCPGKRNEARFTFPKRRFDCRNFPFSSDKRCRWEWERICARIRRRLVQNA
                     RHHRHLNPAGAGKLSSCR"
     gene            complement(4721..7462)
                     /locus_tag="VHR64_02380"
     CDS             complement(4721..7462)
                     /locus_tag="VHR64_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3301698.1"
                     /translation="MMDEARHPSATPTFADLLRSHRIAAALSQEALAEKAGLSVRAIS
                     DLERGVKSHPYLETVRLLADALNLTLPQRATLATVARPVPEADRPQITPVARPGLSPA
                     LPIPPTPLVGREADVAELVDRMHDESLRLVTLTGPGGVGKTRLALEVARQLDDLYVDG
                     IVWVELAPLADSALVAVTVAQALGVREDPGRSLIETMRDRLRGRHILLVLDNCEHMRQ
                     TVATLASELLTGSPGLAILATSRSLLRIAAEQAFPVEPLSLPDTERDPQIDDIAEAAA
                     VRLFVQRVRAVRPSFNLTEQNAADVARICRRLDGLPLAIELAAARVRLLSLPALWALL
                     EERLWVLTGGPEDLPERQRRIRDTIVWSHDLLSPEQQVFFRRLAVFAGGFTLEAVASV
                     AAEGDPFAALTGLEDLVDQSLVRSAEGRHGELRFQMLETVREFALEQLDQSGETEQIR
                     RRHAEYFVLLAKEAKAEQTSLISSGRNRISSERDNVRAALRWCIERGEAEPGLCLAGL
                     LWEVWFRWGDLTGARALITELLALPGAETHPAAWAKAIGAAGALAQAQGDHDPAIALS
                     QRSLDASRVVGDPGDIAAALYTLGLEAMVRGQYQQATEHLEESRRLFSAASDRRVGYW
                     ALRHLSSVRYRRGRMLEAASLAEEGLAVVRAAGSPTEIAGLLHTLGLSTAGSGDLDRA
                     ATLWDECWRLFDETGDLWGVANALGSLGWVAYQRAEFDTAGRQLEDSLKLYEDVGDPE
                     GVTLQLTRLGWLARAQGDIERASELFEQSVALAVKHDNPSTLAPALLGAGAVALDMDQ
                     PARASTLWREALGMANDFGEDDMVASVLEWYAHLAVLQSAEWGARLLAAASALRGEIG
                     ATMSPIDAKEHKPLATTLSSLINATTTAASESPGPEPKAEQAIIEAVSSVLTATHDA"
     gene            complement(7890..10310)
                     /locus_tag="VHR64_02385"
     CDS             complement(7890..10310)
                     /locus_tag="VHR64_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3301699.1"
                     /translation="MRDEAQHLSSTPTFAETLRRYRIAAALSQEALATRAGLSARAIS
                     DLERGVKTRPYLETIRLLADALELSPADRAALTTAARPEPVPARTIIPMIEQPNLPNP
                     PTPLIGREEDIDRVSALIGRDDVRFVTLVGPGGVGKTRLALAVAEQLQSKFAAGVVFV
                     PLAALDEPEMVTLTLAQCLGLRNEQPEMLLDRLAAFLQDKRLLLVVDNVEHVLPAMAQ
                     LVDLVRRSGCAKLLVTSRAVLHLSGEHLHMVKPLPVANAAAAAREIGQSPAVRLFVSR
                     AAAVRPTFMLTEENAPTVAAICERLDGLPLAIELAGARVKLFSPSGLLDRLDAQPALL
                     ATTMHDVAPRHRSFREAVAWSHALLSGNEQRMFRRLSVFRGGWTIEAAEQVANLDDLS
                     HPLETLIALVDQSLVQPTLEDSPEPRFRMLATVREFAAERLSESGEEVFVRNRHSLFF
                     TELAEAAERSFWGPGEIAWLHRADSELENFRAAIRWSAGGHGDPEHAMRLASAIWWMW
                     QTRFGHAEGRAWLEHSIAQGRDVSITVKAQTLATAGMIACFQGDYSMAERWLRDALAL
                     AREAADTRTIAWSTLWLGGTSIFQGEFATSLSLISEGYRLFESIGDAPWTAYSSFYLG
                     VYFALTHDAEQSVAHFSDALRRFREIGFASGIAATLGNMGAFELKLGDRRKAEPRLRE
                     ALSMRFPLKDRWGLAQELRELAQVAALDRDAVRGVRLYAASEALLDSLQTQPPASFMA
                     YWSENEAWLKAQSEDPAYAESWRTGYTQPVETAMAEVRALAVSSVDSIDYLGWPEPGE
                     SIDPTMSR"
     gene            10831..11127
                     /locus_tag="VHR64_02390"
     CDS             10831..11127
                     /locus_tag="VHR64_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301700.1"
                     /translation="MDDLPAWRARPARSARGHGAIQLLLAMVIAAALAWVVFDAASAA
                     FPSSPCSFRASTQYTSVRPLQRFRQRIIPGQPAPLSCPRQPMDPFHQNLALIGR"
     gene            11908..12303
                     /locus_tag="VHR64_02395"
     CDS             11908..12303
                     /locus_tag="VHR64_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301701.1"
                     /translation="MVMERAERQSAERVTGITNLTYDLITLLQNKLEAITAYEVYKQD
                     AQEAGNTRALSIFEHCQDVDRAFVQRLRRLLAEELGTGLTDEAIEDRGTPVQRGGDLS
                     SIRSEEVVDDASEDSFPASDPPSFSGTTT"
     gene            12386..13687
                     /locus_tag="VHR64_02400"
     CDS             12386..13687
                     /locus_tag="VHR64_02400"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX3301702.1"
                     /translation="MASLEGLSIFERGELPRWVKVRQRLDDTDVGNIAEVIAAEFARP
                     GIGDTLAPGMSVALTAGSRGIHAIDQILKACVDEIKKRGATPFIVTAMGSHGGATVEG
                     QLSIIHHYGITEEAMGCEIRASMETVQLGHVEGDVPVWFDRTAYEQADAVIPVGRVKP
                     HTDFVGPVESGLMKMLAIGLGKQKGAEYFHARGFKHFDHLIPAVATFTLSKVNIPFGI
                     ATVENGYSHCSLIEAVPAAQIWDREQELLTIARERLGRLPGQELDVLIVDEIGKDVSG
                     DGADPNVINRDVVGLVDREALGFRPTIQRIIFRDLTPDTEGNATGVGMAEVVLKQLVD
                     KIDPLKTYMNMITAKGPEGARIPMTMENDRRALYIALACCLDTKVETARIARIRSTKY
                     VEDLWVSEPFLEQVLATGRAEVINEPGPILFGANGMFTEGW"
     gene            13720..15051
                     /locus_tag="VHR64_02405"
     CDS             13720..15051
                     /locus_tag="VHR64_02405"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3301703.1"
                     /translation="MLEQLKDVVASQMQIWGVPGVSIGLLKNGETETASYGVASIATN
                     QQVTPETLFQIGSISKVFTTTLVMTFVDDGTLDLDTPIVTWLPDLPLTDGDARESITL
                     RHLVTHMGGFYGDRFDDQGIGDDALAKAVAAFYDLPQQTRPGELWTYCNAGFDLAGRV
                     MEVVSGKRFEDLMRERVFNPLGLQSTTYFAAEAIRHPVSVGHDGDPGELRISDPWPIP
                     RRSNPAGGISSTAPELLRFAQMHLNDGELDGVRVISAESARAMRAKEADAGPFQTWGL
                     GWERVDFGSEVGVRHGGATNGFTAGLLVLPERKVALAILTNHDMGGAAHSAITQAAVR
                     RWFGLERMCAPVVTIPSDQLRVRNGIYTHRLGDLILSANGGSFHVDTVNRNPFDHSEE
                     PGKSFTLTPISEDVFVATGGGRDGSFADFIRNPDGTIRFLRFGGRLAYPKG"
     gene            15099..15311
                     /locus_tag="VHR64_02410"
     CDS             15099..15311
                     /locus_tag="VHR64_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3301704.1"
                     /translation="MTNEVRRLRMEHGLTQQQLAEAMCVSRQTINAIEAGRYTPSLPL
                     AIALARFFKSSVEEMFGESSEQPTPV"
     gene            15280..>15661
                     /locus_tag="VHR64_02415"
     CDS             15280..>15661
                     /locus_tag="VHR64_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301705.1"
                     /translation="MNPRSNQLPYDAGSGHGARWQSWRIFPATYAALGVVILAARIKD
                     GHAADGLIWFGVMAAIAAIYGFGGRFDLIRQARGDLSDEREVSINTHAMAATGTVLVV
                     VLTLCIAWELARGHNPTPYSSLMAV"
BASE COUNT         2817 a         5232 c         4903 g         2709 t
ORIGIN      
        1 gatgacgatc agcgcgggcg ccatggtgat ggctcgccgc acgaaggtgg gaatgcgcca
       61 gcccaggaag ccgcccatca ccacctggcc cgccagtgtt ccgaccgtgg atgatgagag
      121 gcccgacacc agcagcgcca caccgaagag ctcggatgaa gattgtccta gcagcggcgt
      181 cagcgtgcga taggcctgat caagctgggt gacttctgtg tgacccgacg agaagaagac
      241 tgccgctgcg accaccagca tcgcgccatt caccagcccg gcgataccca acgccagcca
      301 gatgtcgaag cgctccagtt tgaagacccg ccgcgcgtcg gcgacgctgc gcaggggaat
      361 gcgtccctgg gtcagcgcag agtgcagata gatgacatgc ggcatgaccg tcgcgccgag
      421 gatgcccacc gccagcagga cgctctcggt cccgtcgaac tggggagtga ccgctccgtg
      481 ggcgatcgcg ccccaatcgg gattgccgat cagggtctcg agcaggtaac agaccgcgat
      541 cacgccgacc atgaccgtga tcaccgcctc gagccgccgg aacccgtgcc gctgcaggcc
      601 gagaatcagg aaggtcgcaa ccgccgtcag cagtgccgcc gggaagagcg ggatatcgaa
      661 gagcagattg aagccgagcg ccgccccgag gaactcggcc aggtctgtcg ccatcgcgac
      721 cccctcggcc acgatccaca ttccgtagac caccggccgc gggaaccgct cgcgacagac
      781 ttcggccagg ttccggccgg tcgcgatgcc cagcttggcc gacagcgatt ggaccagcat
      841 cgccatcagg ttcgaggcga cgatcaccca gaccaggagg tagccgaact cggcgccggc
      901 ctggatattg gtggcgaagt tgccgggatc aacgtaggca acggccgcga tgaaggccgg
      961 cccgaggaag ggcgacagtc gcgcgtgcca ggaccgttcg ctgtggcctg cgaggatttc
     1021 cgaggcggcg acgcgaatat cggcgtcgct cagaccggaa gacttggcaa cgtcggtgtc
     1081 agatactcgt cgcgccacgc cttatttccg ttccggtctc ttctctggtg cgcatttagc
     1141 cgagactaaa gtaatcctgt cgaggcaggc tgtcaaacgc ggcgaccact cgtctgcagc
     1201 ctgaaaacag ttccgctccc tgcgcccgcc cgataatgct cttccatgcc gaacaccgta
     1261 ccggagcgcg ccaatgcagt ctgaccacta cgacgccttg atcatcggcg gcgggccggc
     1321 tggtctctct gccgcaatct acctggcgcg ctccctccgc tcggttgtgg tcatcgactc
     1381 cgatcgcccg gcgcggtcgg actgggtcca ggtcaatcac aacttcctcg gctttcccgg
     1441 cggtgtttcg atcatcgagc tcggccagcg gggacgggag caggctgagc aattcggcgc
     1501 gcgcatcatc aatgccgagg tgacgtcgct gactcaggca gacggccatt tcgaggcggt
     1561 ggtcgacagc gaggtagtgc ggggccgggc ggtcatcctc gccaccggcg tgcgcgatcg
     1621 ctgggtcgaa ttcccgggct acgaggagtt catcggccgg tcgatgcact cgtgcatcgt
     1681 ctgcgacggt tacgagatgc gcggccagcg ggtgttgatc gtcggcaatg acgagcacgc
     1741 cgccatcgtc tcgatccaga tgtgccgctt taccgaccgg atcacgctgc tcattcaccc
     1801 ggacgcgccg ccgctcccga agccactgca ggcggagctg gcgcggtcag ggattcgctg
     1861 cgtgaccggc gaaatcgcga ccgcccgggc gcgcgagccg ggttacttcg aggcggtcgt
     1921 cctggccggt ggcgaggaga ttgagctcga tcacctcttc agcgagcagg gagccgagcc
     1981 gaatgtcgct ctggcgcagg ccctcgacat ggccctgacc gacgagggct acatcaaggt
     2041 cgacaccgaa gcgcggacca gcatcccgta catttacgcg gccggcgatg tcactcgcct
     2101 cttctcgcac caggtcatca cggccgccca cgaaggcgcc accgccgcga caaccctggc
     2161 ctgggacctc ttcgcccagg accgcactaa ccgaaggccg taggtccaga caccggcgag
     2221 caggagcggc gcgcagagat cacgcctggg gctggagcga gtcggcgatg gtgagcgcct
     2281 cgtgcacgat ctccctgatc ggtttcgaga gcgcgtcttg ccagtacgcg tggaacgcgt
     2341 ccggaccgag cccctccttg atccgggcca gcgtcgactg gtactgcttc tcggcgaccc
     2401 tgttccgtac gccaccgaag gcggagttga gcgcactcga tgtcccaagc agtcgagccg
     2461 ccggttcgag ttggttcgtc aacgacactg cttcgccgag acagacaaga ccggccgcta
     2521 cgagagaccg atcatcgatc tccagggcaa ggaggagttc atcacggtag caggcaacag
     2581 ctttcgccgg attcccgagc cgatgctccg acactccgag ctggagcaac gccccgcaga
     2641 ttccccagcc gtggtctatt gactgccaaa ggcgcaatgc ctccctgtag tgttcccggg
     2701 cgcgaatcca gtcgcgttcg tcgtatgcgt ccgccaggcg gtaatgagcc aacgccagaa
     2761 tatgggtgtc gccgaagtcc cgcgcatggg tgagcgcctc ttcgcccaat tgggccgctt
     2821 catccagccg tccctggcag ctgatgatca atccgaggat cgacaggccg gtcacgatgc
     2881 cccattcatc gtcagtggcg cgggcaatgc tgagcaactc gttgccgagt cgctccgcag
     2941 ttgcaaaatc ccctctcagc cgagcaaggc tcgcggctga agcgaggccc tggcatcgta
     3001 tcgagatgga cagatggccg gcggctaatc ctcgttccag ccacacgctt ccttcgcgca
     3061 actctccgcg cgctaaccag tatctccaca gagataccgc gatcccgaga accagctcgc
     3121 cgtcgccgtg atcaatcccc caggtcattg ccccgcgcaa attcggcagg tccgcggtca
     3181 gccgatcaag ccatgccttc tgctctgatc cccgcatgcc tggttccgct gcctgggcca
     3241 gggcacaaaa gtagcgtgcg tgtcgctcgc gaatggtgcc tgcctcggcg ctttgatcga
     3301 gcgcacgttc agcaaactgt cgaacggtct ccaacaggag aaaccgcgtc tctcccgctg
     3361 ggccatctac ctgccgaaca agattttcgt tgaccagaac ggaaatctga tcgtgcaagg
     3421 ccggcgcatc ggtgtcgctg gtcacggtgg tcacggcatt cagggagcac ccgccggcaa
     3481 aaacgccgag gcgccgaaaa acgcgctgat gctgggagga gaggagggcg taactccacg
     3541 caatcgtgga ttcgattcgg cgctggcgcg cgggggcatc cgccggcccc tcacccagta
     3601 cctccaaatg cgttccaatg agcgcgccaa gcgcggctgg cgtcagtact ctcatccggg
     3661 cggcggcgag ctcgattgcc agcggtaaac cgtccaggtg acgacaaatt cgaacgacgg
     3721 aaccgacatt gttctcgtcc agctcgaaat cggatctcac ggtcttggcg cgggcgacaa
     3781 acagggacac tgcatcgttc tctcgaactt cgccgagcag gattggacgg tcgggatcag
     3841 gaagatccaa tggctcgacc cggaacaagc gctccgcgtc aatgtccaag ggtattcggc
     3901 tggtcgccat caacgttggt cccgggaccg cggtcagtaa ggcggctgat tcgcttatcg
     3961 cgtccagaag atgctcgcag ttgtcgaata tgaccagtaa ctgtcgtccg tcgacccgct
     4021 cacgaacgct gcgggccagg gattgatgat ccgcatcacg cgcgccaagg gaaagcgcaa
     4081 tggtcgggag cacgagattc ggatcatcga ggggcgcgaa atcgacccag acgatgccgc
     4141 tggaaaattg cacggtcatc cggcgggcgg ttgcaagcgc cagtcgcgtt tttccgacgc
     4201 ctcctgtccc ggcaagcgta acgaggcgcg tttcacgttc ccgaagcgcc gcttcgattg
     4261 ccgcaatttc ccgttctcgt ccgacaagcg gtgtcggtgg gagtgggagc gcatctgcgc
     4321 ccgtatcagg cgccggctcg ttcaaaacgc tcgacatcac cgtcatttga atcctgccgg
     4381 cgcgggcaag ctcagctcgt gccggtagtg tcaggtcgag ggcgtctgcc agcaggcgga
     4441 cggtttcgag gtagggacgc ttcttgacac cgcgctcgag gtcgctgatt gcccgcacac
     4501 tcaatccagc acgagcggcc agcgcttcct gactcagcgc cgcttcgatc cggtgccgct
     4561 tgagcagctc cgcgaatgtt ggggtgcctg atgattgcgg tgcctcgtcc gccatggtgc
     4621 gccgcccatg tttatttaca ccactaattg tttgaatcgt acccgcctgc gccgtgcgcc
     4681 gcaatccaga tcattgggtc agtgcgacag ccgtacctga tcaagcgtca tgcgtcgcgg
     4741 tcaggacaga cgagacagcc tcgatgattg cctgctcggc cttgggctcg ggacccggtg
     4801 attcgctggc tgccgtggtt gttgcgttga tgagcgacga aagggtcgtc gcgagcggct
     4861 tatgctcctt cgcatcaatc ggcgacatgg ttgcgccgat ctcgccgcgc agcgccgagg
     4921 cagcggcaag cagtcgcgcg ccccattcgg ccgattgcag gacagcgagg tgcgcgtacc
     4981 actccaggac ggacgccacc atgtcgtcct cgccgaaatc attggccatc ccaagcgctt
     5041 cccgccagag cgtcgatgcc cgggcgggtt gatccatgtc cagggccacc gctcccgcgc
     5101 cgagcagcgc cggcgcgagc gtcgacgggt tgtcgtgctt taccgccagg gcgacgctct
     5161 gctcgaagag ctctgaggcg cgttcgatat ccccttgcgc gcgcgccagc cagcccagtc
     5221 tggttagctg cagggtcacg ccttccgggt cgccgacgtc ctcatagagc ttgaggctgt
     5281 cttcgagctg cctgccggct gtgtcgaact cggcccgttg gtatgccacc catccgaggc
     5341 tacccagcgc gttggcaacc ccccagagat cgcccgtctc gtcgaagagg cgccagcact
     5401 cgtcccagag cgttgccgcc cggtccagat cgccggagcc tgcggtcgag agcccgagcg
     5461 tgtgcaggag cccagcgatc tcggtgggag agccagccgc ccgcacgacc gccaagcctt
     5521 cttcggcgag cgatgccgcc tcgagcatcc tgccgcgccg gtaccggacc gagctgagat
     5581 gccgcagggc ccaataccca acccgtcgat ccgaggccgc ggaaaagagc ctccggctct
     5641 cctccaggtg ctccgtggcc tgctgatact ggccgcgcac catggcctcg agcccgaggg
     5701 tatagagcgc cgcggcgata tcgcctggat caccgaccac ccggctggca tcaagcgacc
     5761 gctggctcag cgcaatggcc gggtcgtggt ctccttgcgc ctgcgccaga gccccggccg
     5821 caccgatcgc cttggcccag gccgcggggt gggtttcggc gccgggcagg gcaagcagct
     5881 cagtgatcag ggcccgtgct ccggtgagat ctccccacct gaaccacacc tcccacagga
     5941 ggccggcaag gcacagaccc ggctctgctt cgccacgctc gatgcaccac cgcagcgccg
     6001 ctcggacatt gtcccgttca gatgaaatgc ggttccggcc cgacgagatc aggctggtct
     6061 gttcggcttt cgcctcctta gcgaggagga cgaaatactc tgcatgacgc cggcgaatct
     6121 gctcggtctc accgctctgg tccagttgtt ccagcgcgaa ctcgcggacc gtctccagca
     6181 tctggaagcg cagctcgcca tgccgtccct cggcggaccg aaccagactt tgatcgacga
     6241 gatcttccaa cccggtcagc gccgcgaagg ggtcgccctc ggccgcgacg gacgccacgg
     6301 cttccagggt gaatccaccg gcgaagaccg ccaggcgtcg gaagaacact tgctgttcgg
     6361 gcgaaagcag atcgtggctc cagacaatcg tgtcgcggat tcgtcgctgt cgctccggca
     6421 agtcctccgg gccgccggtc aggacccaca gccgctcctc cagcagtgcc cagagcgccg
     6481 gcagcgacag gagccgtacc cgcgctgccg ccagctcgat cgcgagtggc agaccatcca
     6541 ggcggcggca aatcctggcg acatcggccg cgttctgctc cgtcagattg aaactgggcc
     6601 ggaccgcgcg aacgcgctga acgaagaggc ggacggccgc cgcttcggcg atgtcgtcaa
     6661 tctgcgggtc ccgctccgta tcaggcaggc tcaacggctc gaccgggaac gcctgctcgg
     6721 ccgcgatgcg cagcagcgac cggctcgtgg ccaggatcgc gagtcccgga ctccctgtga
     6781 gcaactccga tgccaacgtc gcaactgtct ggcgcatgtg ctcgcagttg tccagcacga
     6841 gcaggatgtg ccggccccgc aagcggtcgc gcatggtttc gatcagggat cgtcccggat
     6901 cctcacgcac gccgagggct tgggcaacgg taaccgcgac aagtgctgaa tcagccagcg
     6961 gggcgagttc gacccatacg atcccgtcca cgtagagatc atcgagctgg cgggcaacct
     7021 ccagcgcgag ccgcgtcttg ccgacaccgc ccgggccggt cagggtcacg aggcggagcg
     7081 actcgtcgtg catccgatcg acaagctcgg ccacatctgc ctcgcggccg accaacggcg
     7141 ttggcgggat gggcaatgct ggcgacaggc ccggccgggc caccggcgtg atttgaggac
     7201 gatcggcttc gggaaccggg cgagcgacgg tggccagcgt ggctcgctgt ggcagcgtca
     7261 gattcagcgc atcggcgagc aggcgaaccg tttcgaggta cgggtgcgac ttgacacctc
     7321 gctcgaggtc gctgattgcc cgtacgctca gtccggcctt ttcggccagc gcttcctggc
     7381 tcagggccgc cgctatccga tggctccgga gcaagtccgc gaacgtcggg gttgctgaag
     7441 gatgacgcgc ctcgtccatc atggcctgct tccccaacca accttttggc gctcacggtt
     7501 ggcatattac gcagccatca cgtgcggatc aacctcaatt atccgcaaat cgtgtgagca
     7561 gcatgtgagt agcgtgcctg tgccgcgcgt ggagcgttcg accggatcgc cggaaagtaa
     7621 gcggtacgcc gaactggcga gccaccacgc ccgcagaaag gacgcaccgc gatgaggcaa
     7681 gaaccgtacg ccctccagtg ggccgtagcc gagcagcgcc gtcagcacct cctcagcgac
     7741 gtcgaccgga tgcggcagat ccgcaacgcc caaatggatg atccgcagcc cactgccgcg
     7801 ccatcgagac tcagctgcat cttcgatgct gtgctgaacg gactcgtgcg cgtatcagag
     7861 ggaccgaact ggattggtcc ccgcccgttt caacgcgaca tggtcgggtc gattgattcg
     7921 cccggctccg gccaacccaa atagtcgatc gaatcgacgc tcgagacggc gagagcccgc
     7981 acttcggcca tcgccgtctc gaccggctgc gtatacccgg tgcgccacga ctcggcatat
     8041 gccggatcct cggactgggc tttcagccag gcttcgttct cgctccagta cgccatgaat
     8101 gacgccggtg gctgggtctg caatgaatcg aggagcgctt ctgaggcagc gtaaaggcgc
     8161 acaccacgca ctgcgtctct gtcgagcgcc gcgacttgcg ccagctcgcg gagctcctgg
     8221 gccaggcccc agcgatcctt gagtggaaag cgcatcgaca acgcttcgcg caacctaggt
     8281 tcggcctttc tccggtcgcc aagcttgagc tcaaacgccc ccatgttgcc gagcgtcgcg
     8341 gctatgccgg atgcgaaccc gatttcccta aatcgtctca gggcgtcgct gaagtgcgca
     8401 acgctttgct cggcgtcgtg cgtaagcgca aagtagacac cgagatagaa gctcgagtat
     8461 gcggtccagg gcgcgtcacc gatggattcg aaaaggcggt atccctcgga gataagcgaa
     8521 agagatgtcg cgaactctcc ctgaaagatg gatgtgccgc ctagccataa tgttgaccat
     8581 gcaatcgtac gtgtatcggc ggcttccctg gcgagcgcca gtgcatctcg gagccagcgt
     8641 tccgccatgg agtagtcgcc ctggaagcac gcgatcattc ccgcagtcgc gagtgtttgc
     8701 gccttgacgg tgatggacac gtctcgacct tgtgcgatgg agtgctccag ccaggccctg
     8761 ccctcggcat ggccaaagcg tgtctgccac atccaccaga ttgctgacgc cagacgcatc
     8821 gcatgttcgg ggtccccatg ccctcccgcg ctccaacgaa tggccgcgcg gaagttctcg
     8881 agttccgaat ccgctcggtg taaccatgca atctcgccgg gtccccagaa tgaccgctca
     8941 gcagcttccg ccagttcggt gaagaacagg gaatgccgat tgcgaacgaa tacttcctcg
     9001 ccggattcgc tcagccgctc ggccgcgaac tcccgcacgg tagccagcat ccggaagcgg
     9061 ggttccgggc tgtcctcgag ggtcggctgg accaggctct ggtcgaccag tgcgatcaac
     9121 gtctcgagcg gatgactcag gtcatcgaga ttggcgacct gctcagccgc ctcgatcgtc
     9181 cagccaccgc gaaagaccga aaggcggcga aacatgcgct gctcattgcc ggagagcagg
     9241 gcgtgactcc aggccacggc ctcccggaac gaccgatgcc gaggtgcgac atcatgcatc
     9301 gtcgtcgcca gcagggccgg ttgcgcatcg agtcggtcga ggagtcctga cggcgagaag
     9361 agcttgaccc gggctccggc gagctcgatc gccagcggca gaccatccag ccgttcgcag
     9421 atagctgcca ccgtcggtgc gttctcctcg gtcaacataa aggtcggccg gacagccgcc
     9481 gcccgcgaga cgaagagccg cacggcgggg gactgcccga tctctcgcgc cgccgctgca
     9541 gcattggcca ccgggagcgg cttcaccata tgcaggtgct cgccagaaag gtgcagcacc
     9601 gcccggctgg tcactagcag cttcgcgcag ccgctgcgcc ggaccaggtc gaccagctga
     9661 gccatggcgg gcagcacatg ctcgacgttg tcgaccacga gcagcaggcg tttgtcctgc
     9721 aggaacgccg ccagccggtc aagcagcatt tccggctgct cgttgcgcaa gcccaggcac
     9781 tgggcgaggg tcagcgtgac catctccggc tcatccagcg ctgccagcgg cacgaagacc
     9841 accccggccg cgaacttcga ttgcagttgc tcggcgacgg ccagcgcgag ccgcgtcttg
     9901 ccgacgccac ccggtccgac caacgtgacg aaccgcacgt cgtccctgcc aatcagcgcg
     9961 ctaacacggt cgatgtcctc ctcgcggccg atcagcggcg tcggcggatt cggcaagttc
    10021 ggctgctcga tcatcggaat gatcgtccgg gccgggacag gctccggtcg cgccgccgtt
    10081 gtcagcgcgg ctcggtccgc aggcgacagc tccagtgcgt cagcgagcag ccggatcgtt
    10141 tcgagatacg gcctcgtctt gacgccgcgt tcgagatcgc tgatcgccct cgcgctcaac
    10201 ccggcccgcg tggccagcgc ctcctggctg agtgctgccg caattcggta gcggcggagc
    10261 gtttccgcga acgtcggggt gctggacaga tgctgtgctt cgtcgcgcat ggttcgccgc
    10321 ccgtctttgt ttgcgtcact gacagtttga atcgtacgca tggatcacgt gcggcgcaaa
    10381 tccactcccg caaaccgtgt gggcaatgtg tgggttgcgt gcatggttcg acaggcgcgt
    10441 ttggcggaaa gtgacagtgc gcaacgaagc gcgagcatcg gaggtccgca atgaatacgc
    10501 cgtacgcatc ccgtggcccc gccgcggcgg gttaccggca tttcacctgg ttcgacctcc
    10561 agtctgacaa cttcgcgcag tccctggatc aatttgggga ggcgcggagg tcgccccgga
    10621 gcctctttcg cgaggcgatg cgctggctcg gcgccggcgt catgcgcgca ttcaactctc
    10681 cgcgggtccg ccaggccggc tcattgccac gttatacccg cgacggtctt ctttaggtgt
    10741 ccggcatgtc cgcaatcatt ggggaggaac gtagccatgc accccgcgac gcctccagac
    10801 catcgattcc gagaccggca tgcacggtcg gtggacgacc tgcccgcctg gcgcgcgcgg
    10861 cccgctcgat ccgcccgcgg ccacggcgca atccagctgc tgctggccat ggtgatcgcc
    10921 gcggcgctcg cctgggtcgt gttcgatgcg gccagcgcgg cctttccgtc ttcgccctgc
    10981 tccttccggg catcgacgca gtacacgagc gtccgcccgc tccagcgctt ccggcagcgg
    11041 atcatcccgg ggcaacccgc tccgctgtcg tgcccaagac agccgatgga tccattccat
    11101 caaaacctcg ccctcattgg caggtaacaa ggagcacggt cgtgcagaaa gtgacgacac
    11161 cttctcattt gaaactcgcg gaccggcgtc aatctctgcg tcgcggtcta caccatcaag
    11221 ccgattccgc agtttctggt ccgcgtcagg gatggctccc gccagacctc agccatctgc
    11281 tcggtccggc agatggcagc gcggcccggg cgttgtggac caggagcggc gaagggtatc
    11341 cgccgctccc ggcccagcgc tccggcagga gtcttcacat gtcggccgcc ggcaatgcat
    11401 tggggtaccg ggaggagtga aatgcacatc tgtcgtcaat tccaacgcaa tgtatggcgt
    11461 cattgaacgc tgactccgcg cggtaggctc gcacggcgga atcgctctcc agcctaacca
    11521 gtccgccgct tgccgcgcca aatccttact cacgggctcc cgaccctccg ggagcccgta
    11581 ctcttgcacc ggagtacatt tgggacgacc agccagaggc cgtactggtc ggaaactgtt
    11641 gccgcagggt acagttaatc gtgccccgaa ccgaggtcaa tggcgtcctc agttcgtggg
    11701 cggcggagga cccctctttc gcacatgccc atatcacaag cctgaataca gagttccggc
    11761 cgagttatgc caagcgctgg tagcgccgcg catcgcggat cccgtgccat tcgccctagc
    11821 atagattcgg atcatccctg cccgcgctcg cggagtgcgg cgtagcaacc gataccggac
    11881 aaggatgtca ggcaaggagt aagaaccatg gtcatggagc gagcggagcg acagagcgct
    11941 gaacgggtca cgggcatcac gaatctgacc tacgacctga ttacgttgct gcaaaacaag
    12001 ctggaagcaa tcaccgccta cgaggtctac aagcaggacg cccaggaggc cggcaacacc
    12061 cgcgccctct cgatcttcga acactgccag gatgtcgacc gcgcctttgt gcagcgcttg
    12121 cgccgattgc ttgcggaaga gctcggcacg ggtttgaccg acgaggcgat cgaggatcgc
    12181 ggcactcccg tccagcgcgg cggagacctc tcgtcaattc gctccgaaga ggtggtcgac
    12241 gacgcctccg aggattcctt cccggccagc gacccaccct ccttcagcgg caccaccacc
    12301 tgatacggat cgcgcccgcc tgaacctgtc ctgcacctgt atcatgacgg ggtaaggcat
    12361 tcgcatcccc ggtctggagc tggccatggc atctctcgag ggtttgtcga tctttgagcg
    12421 cggggagttg ccccgctggg tcaaggtccg ccagcggctc gatgacaccg acgttggcaa
    12481 catcgccgaa gtgatcgccg ccgagttcgc ccggcccggc atcggcgaca ccttagcgcc
    12541 tggcatgtcg gtcgccttga ccgccggcag ccgcggcatc catgccattg atcagatcct
    12601 caaagcctgc gtcgacgaga tcaagaagcg cggcgcgacc cccttcatcg tcacggccat
    12661 gggcagccac ggcggcgcga cggtcgaggg ccagctcagc atcatccacc actacggcat
    12721 cactgaagag gcgatgggct gcgagatccg cgccagcatg gagacggtcc agctcggcca
    12781 tgtcgagggt gatgtcccgg tctggttcga tcgcaccgcc tacgagcagg ccgatgccgt
    12841 gatcccggtc ggccgggtca agccgcacac cgacttcgtc ggtccggtcg aatccggtct
    12901 gatgaagatg ctcgcgatcg gtctcggcaa gcagaagggc gccgagtatt tccacgcccg
    12961 cggcttcaag cacttcgacc atctgatccc ggccgttgcg accttcaccc tcagcaaggt
    13021 caacatcccc ttcggcatcg ccaccgtcga gaacggctac tcccactgct cgctgatcga
    13081 ggcagtcccg gccgcgcaga tctgggaccg cgagcaggag ttgctcacga tcgcgcggga
    13141 acgactcggc cgcctgcccg gccaggagct ggatgtcctg atcgtcgacg agatcgggaa
    13201 ggacgtcagc ggcgacggcg ccgatccgaa tgtgatcaac cgggacgtcg tcggtttggt
    13261 cgaccgtgag gcccttggct tccggccgac catccagcgc atcatcttcc gtgacctgac
    13321 gccggacacg gaaggcaacg ccaccggcgt cggcatggcc gaggtcgtgc tcaagcagct
    13381 ggtcgacaag atcgatccgc tcaagaccta catgaatatg atcacggcca agggccccga
    13441 gggcgcgcgg atcccgatga ccatggagaa tgaccgccgg gcgctttata tcgcgcttgc
    13501 ctgctgcctc gacacgaaag tcgagacggc gcgaatcgcc cggatccgca gcaccaagta
    13561 cgtcgaagat ctctgggtct cagagccgtt cctcgagcag gtcctggcca ccggccgggc
    13621 cgaggtcatc aacgagccgg gcccgatcct cttcggcgcc aatggcatgt ttaccgaagg
    13681 ctggtaatcc gacccgaata gtcattgcgg gagtaactga tgctggaaca attgaaggac
    13741 gttgtggcga gccagatgca aatctggggg gtcccgggtg tctctatcgg gctgctcaag
    13801 aatggcgaga ccgagacagc cagctatggc gtcgccagca tcgcgacgaa ccagcaggtc
    13861 acgcccgaga cgctgtttca gatcggctcg atctcgaagg tcttcaccac cacgctcgtg
    13921 atgaccttcg tcgacgatgg cacgctcgat ctcgatacgc cgatcgtcac ctggctccct
    13981 gatttgccgc tcactgacgg tgacgcccgc gagtcgatca cgctccgcca cctcgtcacg
    14041 cacatgggcg gcttttacgg cgatcgcttc gacgaccagg gcatcggcga tgatgcgctc
    14101 gccaaggccg tcgccgcctt ctacgacctg ccgcagcaga cccggccggg cgagctctgg
    14161 acctactgca atgcaggatt cgacctcgcc ggccgggtca tggaagtcgt ctccggcaag
    14221 cggttcgagg atctgatgcg ggagcgcgtt ttcaacccgc ttggcctgca atcgacgacc
    14281 tacttcgccg ccgaggcgat ccgccacccg gtctcggtcg ggcacgacgg cgatccgggc
    14341 gagctgcgca tctccgaccc gtggccgatc ccgcgccgct cgaatccggc cggcggcatc
    14401 agctcgactg cgcccgagct gctccgcttc gcgcagatgc acctcaacga cggtgagctg
    14461 gacggcgtcc gcgtgatctc ggccgagtcg gccagggcga tgcgcgcgaa ggaagccgat
    14521 gccggcccat tccagacgtg gggactcgga tgggagcgcg tcgatttcgg tagcgaggtc
    14581 ggcgtccgtc acggtggcgc caccaatgga tttaccgccg gcctgctggt cttgcccgag
    14641 cgcaaggtcg ccctggcaat cctgactaat cacgacatgg gcggcgcggc ccactcggcg
    14701 atcacccagg cggcggtccg ccgctggttt gggctggagc ggatgtgcgc gccggtcgtg
    14761 actattccct ccgaccagct ccgcgttcgc aatggcatct atacgcaccg gctcggcgac
    14821 ctgattctct cggccaatgg cggcagcttc cacgtcgaca ccgtcaaccg caacccgttc
    14881 gaccacagcg aggagccggg caaatccttc accctcacgc caatctcgga ggatgttttt
    14941 gtagccacgg gcggcggacg ggacggctcg ttcgccgact tcatccgcaa cccggacggc
    15001 accatccgct tcctccgctt cggtggccgc ctggcctacc cgaagggatg atgtaaagcc
    15061 aacttgacat cggagtggat gtaaagtagg ctttacacat gacgaacgag gtccgtcgcc
    15121 tgcgaatgga acatgggttg acccagcaac aactggctga ggccatgtgc gtgtcccgac
    15181 agacgatcaa tgcgatcgaa gccggtcgct ataccccgtc gttgccgctg gcaatcgccc
    15241 tggcgcggtt cttcaagtcc agcgtggagg aaatgttcgg tgaatcctcg gagcaaccaa
    15301 ctcccgtatg acgcgggctc gggccatggc gccaggtggc agtcgtggcg cattttcccg
    15361 gcgacttatg ccgctctcgg agtcgtaatc cttgccgcac gaatcaagga tggccacgcg
    15421 gccgacggcc tgatctggtt tggcgtcatg gccgcgattg cggccatcta cggctttggc
    15481 ggtcgattcg acctgattcg ccaagcccga ggtgacctgt ccgacgagcg ggaggtcagc
    15541 atcaacactc acgcgatggc cgcgactggc accgtcctcg ttgtcgtgct caccctgtgt
    15601 atcgcctggg agttggcccg gggtcacaac ccgacgccgt actcgtcgct catggccgtc
    15661 g
//
LOCUS       DATAOZ010000084         9323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2141006, whole genome shotgun sequence.
ACCESSION   DATAOZ010000084 DATAOZ010000000
VERSION     DATAOZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141006"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="VHR64_02420"
     CDS             complement(<1..1249)
                     /locus_tag="VHR64_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEX3301706.1"
                     /translation="MIGRAREHAAVRDLLLRPDVPIVTVTGPGGVGKTRLALHVAADL
                     TDRFADGLRFIPLSAINDAELVVPAIAQGLGLTGLGGQSAEVGLRDYLQDRELLLVLD
                     NMEHVIGAAPALSSLLRDCPSLTLLVTSRETLRVEGEQEYPLKPLAVPDTDTRATAES
                     VAGADAVALFVQRARAAQPDFALDEGNAATVAAICSRLDGLPLAIELAAPRIKMLPPQ
                     ELLNRLANRFNLLSRDARDMPARLRTMRDAVGWSYDLLSLEEQALFRQLSVFGGGFSL
                     EAAEALFDESSEPAGSILERISSLEEKSLVRRVEDSAAPRFGMLETIRRFGFELLVAY
                     GESDAALNAMSAWCQDLFSSGFDQMFGPDQGAWLTIFDAEYDNVRTALGWLLDQGEAA
                     RAQRLSVAMGRYWYVRGYFDEGKN"
     gene            complement(1403..2815)
                     /locus_tag="VHR64_02425"
     CDS             complement(1403..2815)
                     /locus_tag="VHR64_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3301707.1"
                     /translation="MSDFARLSTGRVSRRSFLLASAGLAVPAIVRADPVDPITPAGPD
                     LPIPDTAAGRQLAWVLEQVNRSARGLTLREIREHVDPAFLAVLPADQIRAVLKTYVGP
                     NGPLVPARFEGGVSETRAYVIALNPARQMWRIRLSVSPAEPFLITQLYFEPVSVPFGP
                     ATPVRSWSELTAGIGRLAPRTSFAAAELTEAGPIWFGRLYGDKQRAIASAFKLYVLGE
                     LVRQVASGEVAWQESLAVQDSLRSLPNGDMRLLPAGYELPVQYFAERMIAGSDNTATD
                     HLIGRLGRPRVQAGFADQGHSRPEQNQPLLMTREWFAIRMRLRDTQIERYLAADAGKR
                     LRILADTVDPLAAQLSETEPWPGPRFLDRIEWFAGAADLVRAQHWLWSHSDGPDGAPV
                     RDALSLNCGACWHPDTWRYIGYKGGYETGAMSNTWLLQRQDGRWFSFAAIINDTRREI
                     DANGLWLLQTAAERLLAATR"
     gene            2976..3419
                     /locus_tag="VHR64_02430"
     CDS             2976..3419
                     /locus_tag="VHR64_02430"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX3301708.1"
                     /translation="MSEQDPKEIARRYYEEIFQQRNLDALDQIISPDFRGYSASFGEY
                     TRKDMRRDIQRDHDNMPTDEIIIDEQIAEGDRVTTRWTYRWKHDASLFGEKPTHRWLS
                     MEGVHIDQIADGRIVQRWEVKDFFGVAKQLDLAVGPAIKARSRIE"
     gene            complement(3757..4941)
                     /locus_tag="VHR64_02435"
     CDS             complement(3757..4941)
                     /locus_tag="VHR64_02435"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301709.1"
                     /translation="MATNPIRRIRTRVHETAFDLDIRSTLRRLAALPPSPDVPYLTVS
                     LDWRPEGSVPARVPLGDVQPSQRRTLPRFETISSRPSRQEVERILGELISDFGPRGEA
                     FDSLTADIARLGEVLDDVDPAAQGVFVVACSAHGVFEELSLGLPVPTRAAVGPTPALS
                     VLARLDDDHPPYAVLLADQHQATLSFITQAVVSESVSLDSSGYPRKQKQGGPNQRRYQ
                     NRADERVAAFARGIAEETRKALDETGVGILIIAGDEILTSALDEALHQTVKERVIATI
                     RLDSTASEQEIVDATQSLVRQEERDREAEAVAELRDQIGSDAAAVAGAERTLVTLQGG
                     RVDTLIMVDDLALTGWADFAMGVYGAGDPPQEHPAGGDIATTRASSSLSRPVTKPIRS
                     RP"
     gene            complement(5120..5896)
                     /locus_tag="VHR64_02440"
     CDS             complement(5120..5896)
                     /locus_tag="VHR64_02440"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3301710.1"
                     /translation="MDLRLDGKVALVTGSSKGIGKGIAEELARSGADVTITYEGDESG
                     AEETAEMVRELGRRALIVLVDVADETGVDRLYAAHLAEFGRLDILVNNAAIVSHGRIH
                     EISTDEWRKVMAANLDGAFFCMARAARQMIAQGGNGRIIAISSVHEEACTDGGGPYAV
                     SKAGLRNLVRTMAIELGEYGITVNGIAPGMTVTPGMNTAIYNDEKLREERAQLIVLKR
                     AGYPEDIANMAVFLASDAASYCTGATYYVDGGWMLTHPPV"
     gene            complement(6034..7173)
                     /locus_tag="VHR64_02445"
     CDS             complement(6034..7173)
                     /locus_tag="VHR64_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX3301711.1"
                     /translation="MGHSKVLTQSGCQLRQDRLRALLERRGLDAAILFDANEIFYLTG
                     FLISDFLAQPALLYIEADGSSFLVCATDEGEALVDERLTYEPHLLYTRNLNLIRQIAA
                     LLERRLAGRSVRRIGWRAEALPRMIGLAIDATIAPDEWVAIDDDIADMERAKDPDEIE
                     LIRTSIACNLAAYGAAELEIAPRVSELAVLQAGQRSAILRAGEQVFHSGDYRAGEFGG
                     FARPVPIHEGDLYIIDAWTEYRSYWSDMARTFAVTTATPLQREVYDHIAHILLDVEGQ
                     LKPGVHGTDIWKWVDGRIREHPKFRETGLIHHAGHGVGLRAHEGPDLNRDRGEELRPG
                     DVVSVEPGAYAPELRPGFRLENMFLITETGCELLSEYPLSLERQV"
     gene            complement(7288..8085)
                     /locus_tag="VHR64_02450"
     CDS             complement(7288..8085)
                     /locus_tag="VHR64_02450"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX3301712.1"
                     /translation="MDSVAGVNGTVFCTNCGQKLAPDDQFCPECGTPRPMFSPRETTP
                     PEFTSSVTTATMLGEFTGAPPMMVGTMAPAPVVTGSPLPEYDVEYPERLSRLLIFVKW
                     LLIIPHLIALVFVGIALTVVTILAFFAILITGRYPRGMWEFSLGVLRWGTRVNAYMSL
                     QRDEYPPFSGTAPYPVHLDLVYPERLSRLLIFVKWLFVIPSAIVYWIVSWIAGIVLFL
                     AFFAILFTGRYPRSLFDFTTGAMRWSNRISAYLLLLTDGYPPFRMGP"
     gene            8229..8807
                     /locus_tag="VHR64_02455"
     CDS             8229..8807
                     /locus_tag="VHR64_02455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX3301713.1"
                     /translation="MSNGSKPQLQIIIASTRPGRVGLPVGKWFEQCAIDHGAFEVEVA
                     DLAEIDLPFLDEPNHPSQQRYTKQHTLDWSARIASSDAFVFVMPEYNHSYTAPLKNAI
                     DYLVKEWGFKPIGLVSYGGISGGIRAVQALKPVLMGLQMFVLKEAVVIQFVASLIEDD
                     GTFQPTAAIANSVKPMLDELARVNPVLKQLRK"
     gene            complement(8871..9212)
                     /locus_tag="VHR64_02460"
     CDS             complement(8871..9212)
                     /locus_tag="VHR64_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301714.1"
                     /translation="MAVYSGNAGSILYDGVIIGAVAEWRLLIVTHNDNARWTGSLTWQ
                     WLSLPREDPDDDEFDIELQDGAETPSYNLSGRTVFTSIDLQKRTLDFRGQGDLEGVPD
                     PGPGQSEIAIT"
BASE COUNT         1767 a         3155 c         2880 g         1521 t
ORIGIN      
        1 agtttttgcc ctcgtcaaag tagccgcgaa cataccagta ccggcccatg gcaacggaca
       61 atcgttgtgc ccgcgcggct tccccctgat cgagcagcca gcccagcgca gttcggacgt
      121 tgtcatactc cgcatcgaaa atcgtcagcc aggcgccttg atccggaccg aacatctgat
      181 caaagccgga gctgaacaga tcctggcacc atgcggacat cgcattcagc gcggcgtcac
      241 tctcgccata ggcgacaagc agctcgaagc cgaaccgtcg aatcgtctcc agcatcccga
      301 acctgggcgc ggccgaatct tcgacccggc gaaccagact cttctcctcc agagacgaaa
      361 tgcgctccag aattgaacca gccggttccg aactctcgtc gaacagggcc tccgccgctt
      421 cgagcgagaa tccgccgccg aatacagaca actgccggaa cagcgcctgc tcctcgagcg
      481 acagcaggtc gtagctccag cccaccgcgt cgcgcatggt gcgcaagcgc gccggcatgt
      541 cccgagcgtc ccggctgagc agattgaaac gattggccag cctattcagg agctcctggg
      601 gcgggagcat cttgatgcgc ggcgcggcaa gctcaatcgc gagcggcagc ccatccaggc
      661 gggaacagat ggccgccaca gtcgcagcgt tcccctcatc gagtgcaaag tccggttgcg
      721 cggccctggc tcgctgaacg aagagcgcga ccgcatctgc gccggcaacc gattcagccg
      781 ttgctcgcgt gtctgtatcc gggactgcca gcggcttgag cgggtactcc tgctcgccct
      841 cgacccgaag cgtttcgcga ctcgtcacga gtagcgtcag actgggacag tcccgcagca
      901 agctcgaaag cgccggggcg gcgccaatga cgtgctccat gttgtccagc accagcagca
      961 actcgcgatc ctgcaagtaa tcccgcagtc cgacttccgc tgactggccg ccgagcccgg
     1021 tcaagccgag accctgcgcg atcgcgggga caaccagctc cgcatcgttg atcgccgaga
     1081 gcgggatgaa gcgcagaccg tccgcaaacc ggtcggtgag gtcagccgcc acatgtaggg
     1141 ccagccgggt cttgccgacg ccgcctggcc cggtcacggt cacgattggg acatcggggc
     1201 ggagcaggag atcacgcacc gcggcatgct cgcgcgcgcg gccaatcatc ggtgtccgcg
     1261 ggacagggag gagcatgcct gattcaggag cggaaggact ggacattgcc gcgcaccgca
     1321 catgaatcgt ttatcgggta ttgcggcaat cctgtcataa acggcgcgag tggtcaaacg
     1381 ctcgcaattg gggcaccgtg cgctatcggg tcgcagccag taaccgctcg gccgcggttt
     1441 gcaggagcca caacccattg gcgtcgatct cgcgacgggt gtcgttgatg atcgcggcga
     1501 aggagaacca ccgcccatcc tgccgctgca ggagccaggt gttgctcatc gcgcccgtct
     1561 catagccgcc cttgtacccg atgtaccgcc aggtgtcggg atgccagcag gcgccgcagt
     1621 tgagcgagag cgcgtcccgc accggcgcgc cgtccggacc gtccgagtgc gaccagagcc
     1681 agtgctgcgc ccggaccaga tccgccgcgc cggcgaacca ctcgatccgg tcgagaaaac
     1741 gcggacccgg ccatggctcg gtctcgctca gctgcgcggc cagcggatcg acggtgtcgg
     1801 cgaggatccg caaccgtttg ccggcgtcag ctgccaggta ccgctcgatc tgggtatcgc
     1861 gcagccgcat ccggatcgca aaccactccc gcgtcatcag cagcggctgg ttttgctcgg
     1921 gccggctatg tccctgatcg gcgaatccgg cctgcacccg tggccgaccg agccgtccga
     1981 tcaggtggtc ggtcgcggtg ttgtcgctgc cggcgatcat ccgctccgcg aaatactgga
     2041 ccggcaactc gtagccggcc ggcagcagcc gcatgtcgcc gttcggcagc gagcgcaggc
     2101 tgtcctggac cgccagcgac tcctgccacg ccacctcgcc cgacgctacc tgccggacca
     2161 gctcgccgag cacgtagagc ttgaacgcgg aggcaattgc ccgctgcttg tctccataga
     2221 gccggccaaa ccagatcgga ccggcctcgg tcaactccgc cgccgcgaag gacgtgcgcg
     2281 gggcaaggcg cccgattccg gccgtcagct ccgaccagct ccggaccggt gtcgccgggc
     2341 caaagggcac gctgaccggc tcgaagtaga gctgggtgat caggaacggc tcggccggcg
     2401 agacgctcag cctaatccgc cacatctgcc gggccgggtt gagcgcgatc acgtaggcgc
     2461 gggtctcact gacgccgccc tcgaaccggg ccgggaccag cggaccattc ggtcccacgt
     2521 atgtcttcag caccgcccgg atctggtcag ccggcaacac ggccaggaac gccggatcga
     2581 cgtgctcccg gatctcgcgc agggtcaatc cccgcgcgct gcgattcacc tgctccagca
     2641 cccacgcgag ttgccgaccc gccgccgtat ccgggatggg cagatccggt cccgccggcg
     2701 tgatcggatc gaccggatca gcccgcacga tggcaggcac agccagcccg gcggatgcca
     2761 ggagaaacga gcgccgggaa acccggccgg ttgaaaggcg cgcgaaatca ctcattgcgt
     2821 tctcagcggg gcaaagccac cgtatcccac tgccccaaaa acgccaatcc cccatccgtc
     2881 gtttcccggt cggtgagcga acgggcagct attccgcgat cgctgagtgc ggaacgaatc
     2941 aggtagggtt tgcgcgactg acgcgaggag aagcaatgag tgaacaggac ccgaaagaaa
     3001 tcgcgcggcg gtattacgag gagatcttcc agcagcgcaa cctcgacgcg ctcgatcaga
     3061 tcatctcgcc cgatttccgc ggctactcgg ccagtttcgg cgagtacacg cggaaggaca
     3121 tgcgccgcga catccagcgc gaccatgaca acatgccgac agacgagatc atcattgacg
     3181 agcagatcgc cgagggagac cgcgtcacca cgcgctggac ctatcgctgg aagcacgacg
     3241 catcgctctt cggcgagaag ccgacccacc gctggctctc aatggaaggc gtccacatcg
     3301 accagatcgc cgacggcagg atcgtccagc gctgggaagt caaggacttc tttggcgtcg
     3361 ccaagcaact cgacctggcc gtaggccccg cgataaaagc acggtcgcga attgagtaag
     3421 atatcgtaac gcgagcttcc gggtttgcca cgttcgacgg cgacagcgag cccgaacaca
     3481 atggagtagg cgggtgtttc aggatttaca cgttttcgac gttcatgcgc actttccgat
     3541 caagggcgat ctgactcagg gcggagggcg cagcctgcgc acccgcgatg tcggccacat
     3601 cgagaacgag gcggtgaagg agcgaatggc cttccgcgag caacaactcg cgcggacccg
     3661 ccagcagtgg cgcctcgcct tcaactttcc cgatcccgag aaggagcccc ggtcgagcga
     3721 ggagcaggcc gcgcgctggg tcgccgagct cgacacctac ggcctcgagc ggatcggctt
     3781 cgtaaccggg cgggacaacg acgagctggc gcgtgtggtc gcgatgtcgc cacccgcggg
     3841 gtgctcctgc ggcggatctc cggcgccgta cactcccatt gcgaaatcag cccagccggt
     3901 cagggcaagg tcgtcgacca tgatcagcgt atcgacccgg ccgccctgca acgtgaccag
     3961 cgtgcgctcc gctccggcga ccgcggccgc atcggaaccg atctggtccc gaagctcggc
     4021 gactgcctcc gcttcccggt cccgttcctc ctggcggacg agcgattgcg tcgcatcgac
     4081 aatctcctgc tcgcttgcgg tgctgtcgag ccggatcgtg gcgatgaccc gctccttgac
     4141 cgtctggtgc agcgcctcgt ccagcgcgct ggtcaatatc tcgtctcccg cgatgatcag
     4201 gatcccgaca cccgtctcgt cgagcgcctt gcgcgtctcc tcggcgatcc cccgcgcaaa
     4261 ggccgcgacc cgctcatcgg cgcgattctg gtagcggcgc tggttcggtc caccctgctt
     4321 ctgcttgcgc ggataccccg agctgtcgag cgaaacgctc tcactgacaa ccgcctgcgt
     4381 gatgaacgag agcgtcgcct gatgctggtc cgcaaggagg acggcgtacg gcggatgatc
     4441 gtcatcgagt cgggccagaa ccgacagcgc cggtgtcggg ccgacggccg ctcgcgtcgg
     4501 cacgggcagc cccagcgaaa gctcctcgaa gacgccatgc gccgaacacg ccacgacgaa
     4561 gacaccctgc gcggctggat ccacgtcgtc gagcacctcc ccgagtcgcg caatgtcggc
     4621 agtcaggcta tcgaaagcct cgccgcgcgg accgaagtcg gagatgagct cgccaagaat
     4681 ccgctcgacc tcttgccggg acggccggct gctgatcgtc tcgaaccgcg gcagcgtccg
     4741 gcgctgggac ggctgcacat cgccaagcgg cacacgcgcc gggaccgatc cctccgggcg
     4801 ccagtcgagg ctgaccgtca gatacggcac gtcgggggag ggcggcaatg ctgccagtcg
     4861 acgcagtgtc gagcgaatgt ccagatcgaa cgccgtctcg tgcactctgg tccgaattcg
     4921 ccgaatggga ttggtggcca tagctgtcgc ctccacttga acgcgggttt ccccgcatga
     4981 atgcgctctg ctggattgtt ttgggatgca catgtgtgcc aggacgatca agtatggccg
     5041 caacggcagg ctccgcagcg agcgatagct accgcggtga cctcaaatca agtgcgcgat
     5101 tgatccgacg atgcgggtcc tataccggcg ggtgggtgag catccagccg ccgtcgacat
     5161 aatatgttgc gccggtgcag tagctggccg cgtcgctggc caggaaaacg gccatattcg
     5221 cgatgtcctc gggatacccg gcccgcttca gcacgatcag ctgcgcccgc tcctctcgca
     5281 acttctcgtc gttgtagatc gcggtgttca tccccggcgt gaccgtcatc cccggcgcga
     5341 tcccgttgac cgtgatccca tactcgccga gctcgatcgc catcgtccgc accaggttcc
     5401 gcaaccctgc cttcgagacg gcgtaggggc caccaccgtc ggtgcaggcc tcctcatgga
     5461 ccgacgagat cgcgatgatc cggccgttcc cgccctgcgc gatcatctgc cgcgccgccc
     5521 gcgccatgca gaagaaggcg ccgtccaggt tcgcggccat caccttccgc cattcatcag
     5581 tcgagatctc gtggatacgg ccgtggctga cgatggcggc gttgttgacc aggatatcga
     5641 gccgcccgaa ctcggccaga tgcgccgcat agagtcggtc gacgcccgtt tcatccgcga
     5701 cgtcgaccag gacgatcaac gcccgccgtc ccagctcccg caccatttcg gccgtctcct
     5761 ccgcgccgga ttcgtccccc tcataggtga tcgtgacatc cgcgccggat cgggcgagct
     5821 cctcggcaat gcccttgccg atgcccttgc tggagccggt gaccagcgcc accttgccgt
     5881 caagtcgcaa atccatgttc ggctgtactc cctgcgcgtg ctgaagcgac ggtcaaacgt
     5941 agtcccgggc ggctcgcctg gcagcgcgtc acgaaaaccg ggcggactgc ccgggactac
     6001 atcatgggat ggttcatgac ccggaaatct gcgctacacc tggcgttcga gtgaaagcgg
     6061 atactcggaa agcagctcgc acccggtctc ggttatcagg aacatgttct caagccggaa
     6121 gcccggccgc aattcgggag catacgctcc gggctcgacc gagacgacat cgcccggccg
     6181 caactcctcg ccccgatccc ggttcaggtc cggtccctcg tgcgcgcgca ggccgacgcc
     6241 atgcccggca tggtggatca ggcccgtctc ccggaacttg ggatgctcgc ggatccggcc
     6301 atcgacccat ttccagatat cggttccatg cacgcccggc ttgagctgcc cctcgacgtc
     6361 gagcaggata tgggcgatgt ggtcgtaaac ctcgcgctgg agcggcgtgg ccgtcgtgac
     6421 cgcgaaggtg cgcgccatgt cggaccagta actccggtac tcggtccagg cgtcgatgat
     6481 gtagagatcg ccctcgtgga tcggcaccgg ccgcgcgaac ccgccaaact cgccggcccg
     6541 gtaatccccg gagtggaaga cctgctcccc cgccctaaga atcgccgagc gctgcccggc
     6601 ctggagtacc gccagctccg ataccctcgg cgcgatctcc agctcagcgg cgccatacgc
     6661 ggccaaattg caggcaatcg acgtgcggat cagctcgatc tcgtctggat ccttggcccg
     6721 ctccatatcg gcaatgtcgt cgtcgatcgc gacccactcg tccggcgcga tcgtggcatc
     6781 aatcgcgagc ccgatcatgc gaggcaacgc ctccgcgcgc cagccgatcc gccggaccga
     6841 gcggcccgcc agccgccgct cgagcagcgc cgcgatctgt cggatcaggt tcaggttgcg
     6901 ggtgtagagg agatgcggct cataggtcag ccgctcatcg accagcgcct caccctcgtc
     6961 cgtcgcgcag accaggaatg aggagccatc agcctcgatg tagagcaatg cgggctgcgc
     7021 gaggaaatcc gagatcagga aaccggtcag gtagaagatc tcgttcgcgt cgaagaggat
     7081 cgccgcgtcc agcccgcgac gctcgagcaa cgctcgaagg cggtcctgac gtagttggca
     7141 tccagactgc gtcaacacct tgctatgccc catccgagtt gcggtccgcg cgtcatgtgt
     7201 ccgtcatcct atcaatccaa aatgccccgg acaatcgggc agatcaccta ggaccaaacg
     7261 ggctggccaa ggaacgaacc gccggcgcta gggccccatc cggaagggcg gatagccatc
     7321 ggtcagcagc agcaaatagg cgctgatccg gttgctccag cgcatcgcgc cggtcgtgaa
     7381 gtcgaacaag ctgcgcggat accggccggt gaacaagatc gcgaagaagg ccaggaagag
     7441 aacaatcccg gcgatccagg agacgatcca gtagacgatc gcgctcggga tcacgaagag
     7501 ccacttcacg aagatcagca gccggctcaa tcgctcgggg tagacgagat cgaggtgcac
     7561 cgggtacggc gcggtgccgc tgaagggcgg atactcatcc cgctgcaagc tcatgtaggc
     7621 attgactcgg gtgccccagc gcaacacccc cagcgagaac tcccacatcc cgcgtgggta
     7681 gcgccccgtg atcaggatcg cgaagaaggc caggatcgta accacggtca aggcaattcc
     7741 gacgaagacc agcgcgatca ggtgcgggat gatcagcagc cacttgacga agatcagcaa
     7801 ccgactaagc cgctcgggat actcgacgtc gtactcgggc agcggcgagc cggtcacgac
     7861 cggcgccgga gccattgtcc caaccatcat cggcggggcg ccggtgaact cgcccagcat
     7921 ggtggccgta gtcacggagc tcgtgaactc cggcggagtc gtctcccggg gcgagaacat
     7981 cggccgcggc gttccgcatt ccgggcagaa ctgatcatcc ggggccagct tctgaccgca
     8041 gttcgtgcaa aagaccgtgc cgttcacgcc ggcgacgctg tccatggatc gcgctcctca
     8101 tgatcgctgc aatacccgga ttctacgcga caaccgctca ccaatagccc gcgcacagct
     8161 agcggattcg gccgtcacta tccttgctct cctgagtccg cgctgccgcg ccacggagga
     8221 gccgccacgt gtccaatgga agcaagccgc aactgcagat cattatcgcc agcacccgcc
     8281 cgggccgggt cgggctgccg gttggcaagt ggttcgagca atgcgcgatc gaccatggcg
     8341 cattcgaggt cgaggtcgca gatctggccg agatcgacct gcccttcctc gacgagccga
     8401 accacccgag ccagcagcgg tacacgaagc agcacacgct ggattggagt gcccgcatcg
     8461 cctcctcgga cgccttcgtc ttcgtcatgc cggagtacaa ccactcctat accgcgccgc
     8521 tcaagaacgc gatcgactac ctggtcaagg agtggggctt caagccaatc ggactggtca
     8581 gctacggcgg catttcgggc ggaatccggg cagtacaggc gctgaagccg gtgctcatgg
     8641 gattgcagat gttcgtgctc aaggaggcgg tcgtgatcca gttcgtcgcc tccctgatcg
     8701 aggacgacgg caccttccag ccaaccgccg caatcgccaa ctcggtcaag cccatgctcg
     8761 acgagctcgc ccgcgtcaac ccggtgctga agcaactgag aaagtagtgg atggtggtta
     8821 gtggttggtg gctggaaaac ggatccaaca accatccacc aaccacccgc ttatgtgatc
     8881 gcgatctccg attggccggg accgggatcg ggcacaccct cgagatcgcc ctgcccgcgg
     8941 aaatcgaggg tgcgcttttg caggtcgata ctggtgaaga ccgtccggcc ggagaggttg
     9001 tagctcgggg tctcggcgcc gtcctggagc tcgatatcga actcgtcgtc gtctggatcc
     9061 tcgcgtggca ggctcagcca ttgccaggtc agcgatccgg tccagcgcgc attgtcgtta
     9121 tgcgtgacaa tcagcagtcg ccactcggcc accgcgccga tgatcacccc gtcatacaaa
     9181 atcgatccag catttccgct gtacacagcc atttttggct ctcggagctc ggccatcgcg
     9241 tccagatcag gtaccttctt gattaagtac ggtagagcat aaggaccaag gagagaatat
     9301 tcaatgacgg accacccgaa ctc
//
LOCUS       DATAOZ010000085         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8192921, whole genome shotgun sequence.
ACCESSION   DATAOZ010000085 DATAOZ010000000
VERSION     DATAOZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8192921"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..>1679
                     /locus_tag="VHR64_02465"
     CDS             361..>1679
                     /locus_tag="VHR64_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301715.1"
                     /translation="MRDRRAIALSSRESQIILIAAALVIFRSAVFMFWPQSYFDSDQA
                     IFGLMAKHIAEGRAFPVFMYGQTYILAVQAWMAAPFVMIAGASVATLKLPLLIINVAI
                     VILLVRIFIRQVGLTPALGLLAAMFFVLPAPGTAAKFVEASGGNVEPFLYIILIWLTR
                     NRPNWCGLILGVGFLQREFTVYGLASLLTLEAAHGVLFTREGIVRRLRMFRTTAEVWL
                     LVTWFKQYSSAAGPGTSLADLYRPPPDNLRELLNRICVDPTTMISGLRTGLSTHLPQL
                     FGMMWQPLADYGIDSSVQQGLPWGSLLFFAIVGIAVVRIAMTIVGERRWRPEYDPCAY
                     LVLAGLFSFGAYTLLRCGVIGVMRYELLSILGATGLAAWYLRVERTRALATVWIALTV
                     AWAAVSGVGHARLWTEYVSHPPVGAKRLIIRHLETRGIRYAYADYWLA"
BASE COUNT          269 a          549 c          495 g          366 t
ORIGIN      
        1 tccgacaggt ccttcttcct gaggctgacg agcgacacgt tgtagcgctc agggtcgaag
       61 cggggaatca tccacgcaaa cagccgtttc accccgtgca tgcgcgatcc ttcccatccc
      121 aggtgatcgc agacctggag aacgtttagc ttcaccggct ctccgcgcca atctcaaccg
      181 agcggcttcg cccctcggca cgcctccgcg taggcttcgg cgatgtgtct gccgtagccc
      241 tcggcgaagg cggaactccc caacacgctc tctcgctatg ctcgctcgca tcgtgaccac
      301 gaacatcggt catgacgcac cttctaaaca ctagagcgct agcatataat accggtccgc
      361 atgcgcgacc ggcgcgcgat cgccctgagc tcgcgcgaat cgcaaatcat cctcatcgcc
      421 gcggcgctcg tcatctttcg ctcggctgtg ttcatgttct ggccccagtc gtacttcgac
      481 tcggatcagg cgatcttcgg cctcatggcc aaacacattg ccgaagggcg tgcgttcccc
      541 gtgttcatgt acggccagac ctacatcctt gccgtccagg catggatggc ggcgcctttc
      601 gtcatgatcg caggcgcgtc ggtggcgaca ctgaaactgc cgctgctcat catcaacgtt
      661 gccatcgtta ttcttctggt tcgaattttc atccgccagg tcggtctcac gccggcgctc
      721 gggctgctgg cggcgatgtt cttcgtgctg cccgcaccgg ggacagccgc gaaattcgtt
      781 gaagccagcg gcggcaatgt cgaaccgttt ctctacatca ttctgatctg gctgacgcga
      841 aaccgtccga actggtgtgg gttgattctt ggtgtcggat tcctccagcg ggaattcacg
      901 gtgtacggcc tggcctcgct cctcaccctc gaagccgcgc acggcgtgct cttcacacgc
      961 gagggcatcg tccgacgctt acgcatgttc cggacgaccg cggaggtctg gttgctcgtc
     1021 acatggttca agcagtactc gtcggccgct ggacccggca cctcgctcgc cgacctgtac
     1081 cggccgccac cggacaacct ccgcgagctg ctgaatcgca tctgcgtcga tccaacgact
     1141 atgatcagcg gactccggac gggcctttcc acgcacctgc cgcagctgtt cggaatgatg
     1201 tggcagccgc tcgctgacta tggcatcgac agcagcgtcc agcaggggct gccgtgggga
     1261 tctctcctct tctttgccat cgtcggcatc gctgttgtgc ggattgcgat gaccatcgtt
     1321 ggcgaacgac gatggcgtcc cgagtacgat ccgtgcgcct atcttgtgtt ggccgggctg
     1381 ttttcgttcg gtgcctacac gctgctccgg tgcggcgtca ttggcgtcat gcgctatgaa
     1441 ctgttgtcga tcttgggggc gaccggtctc gccgcatggt acctgcgcgt tgaacggacg
     1501 cgagcgctcg cgacggtatg gattgcgctg acggttgcgt gggcagcggt cagcggcgtc
     1561 ggccatgcgc gcttgtggac ggagtacgtg tcacatcccc cggtcggggc gaagcgcctg
     1621 atcatccgcc acctcgagac gcgaggaatc cgttacgcat acgcggacta ttggctggc
//
LOCUS       DATAOZ010000086         3474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4509509, whole genome shotgun sequence.
ACCESSION   DATAOZ010000086 DATAOZ010000000
VERSION     DATAOZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4509509"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..2297
                     /gene="polA"
                     /locus_tag="VHR64_02470"
     CDS             72..2297
                     /gene="polA"
                     /locus_tag="VHR64_02470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX3301716.1"
                     /translation="MADYKALVGDTSDNIPGVAGIGEKTAKALIAQYGDVENIITNLD
                     KITPPRARNALAAGVDEARLSKFLTTIVRDLPIEIDLDRSQVGNYDREAIIELFRELE
                     FRTLANRLPEPRSDRAAAPPRIERPPSVRTIVRTEDDLSRLVQRVRDVGRYAIDVETD
                     SLDPLSANLVGIAIGVGPAEGFYIPLSHAVGNPEQLSQEEVRDALRPLLVDSNLEAYA
                     HHAKYDLAVLERHGYEVSNLGFETMIAAYLLGETSVGLKDLAFTRLGREMTEIVALIG
                     SGSKQLTMDNVDSDQAGDYACGDVEATFELADTFRPEIHDAGMASLLFEIEQPLVPIL
                     VEMERAGIAVDAPYLNELSREVTTRLQQVEVELNELAGRSINPNSARQLAPLLFDELK
                     LPSGRRTKTGFSVDSEVLENIRHQHPIVELILEHRTLAKLKSTYVDALPLQVNERTGR
                     VHTSYNQTVAATGRLSSTNPNLQNIPIRTELGRRVRRAFVADTRPDFRLFDDSVLVSA
                     DYSQLELRLLAHLSEEPFLIDAFARGDDIHTATAAVIHGVPRGDVTPDMRRVAKTVNF
                     GIIYGMQAHGLSRDTGLSRQEAQRFIDQYWTNLPQVKRYFDGTLAFGIKHGYVESIYG
                     RRRILGDLTSANYTRRAAAERMAVNMPLQGSASDIMKLAMIKLDRELARSGLRAKLLL
                     QVHDELVLEVERADVPAVADLLMQTMERAVDLRVPLETEVRAGPNWDDLAPIEALAVS
                     A"
     gene            2458..2745
                     /locus_tag="VHR64_02475"
     CDS             2458..2745
                     /locus_tag="VHR64_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX3301717.1"
                     /translation="MPTYTYRCDTCGHGFEAVQRFADEPLKDCPECGAPIRRVIQPVG
                     VVFKGSGWYITDSRPKSSSDSGDGASKTDKSDKSEKRDSAKTDKTPAKVAD"
     gene            2931..>3474
                     /gene="epsC"
                     /locus_tag="VHR64_02480"
     CDS             2931..>3474
                     /gene="epsC"
                     /locus_tag="VHR64_02480"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEX3301718.1"
                     /translation="MLFAKEIRAIRERDPASRSAIEAALTSPGLHAIALHRLSHWLWQ
                     KGLFLPARLLSQLSRFITGIEIHPGARIGQGVFIDHGMGVVIGETASVGNNVTMYQGV
                     TLGGTGKERGKRHPTVECDAVIGVGASVLGAVTIGEGARVGAGSVVLKDVPPHTTAVG
                     LPARAVSWRDPHSGETRRLEH"
BASE COUNT          671 a         1130 c         1053 g          620 t
ORIGIN      
        1 agcgcttcgg agacctgcgc tacttcgacc cggccgcggt ccatgcccgc tacggttttg
       61 gacccgagca cgtggccgat tacaaggcgc ttgtgggcga tacgtcggac aatatccccg
      121 gagttgccgg catcggcgaa aagaccgcga aggccctgat cgcacaatac ggcgacgtcg
      181 aaaacatcat tacgaacctg gacaagatca ccccgccccg ggccaggaac gcgctcgcgg
      241 cgggagtcga cgaagccaga ctcagtaagt tcctcacgac catcgtccgc gatctgccga
      301 tcgagatcga cctcgatcgc agtcaagttg ggaactacga ccgcgaagcc atcatcgagc
      361 tctttcgcga gctcgagttc cgtaccctgg caaaccgcct gccggagcca cgctccgacc
      421 gcgccgcggc cccgccacgc atcgagcgac cgccatcggt acggaccatc gtgcggactg
      481 aagacgatct cagtcggcta gtgcagcggg tgcgcgatgt tggccgctac gccatcgacg
      541 tcgaaaccga ctccctcgac ccactctccg ccaacctcgt tggcatcgcc atcggcgtag
      601 gaccggcgga aggcttctac atcccactct cccacgcggt cggcaacccg gaacaactct
      661 cccaggagga ggtgagagac gcactgcggc ccctgctggt cgattccaac ttggaagcct
      721 acgcccacca tgccaaatac gatctggccg tcctggagcg gcacggctac gaggtgtcca
      781 accttggttt tgaaacgatg atcgccgcct acctgcttgg cgagacctcg gttggtctca
      841 aggacctggc ctttaccagg ctcggccggg agatgaccga gatcgtcgcc ctgatcggga
      901 gtggcagcaa acaactaacg atggacaacg tcgactccga tcaggccggt gattacgcct
      961 gtggtgacgt ggaggcgacg ttcgagctag ccgatacatt ccgccccgag atccacgacg
     1021 cgggaatggc gtccttgctg ttcgaaatcg aacaaccgct ggtaccgatc ctggtggaga
     1081 tggagagggc tggcatcgcc gtcgatgctc cctatctgaa cgaattgtca cgggaagtca
     1141 ccacacgcct acagcaggtc gaggtggagc tcaacgagct ggcggggcgg tcgatcaatc
     1201 cgaactcggc ccggcaactg gcgccactgc tcttcgacga gctcaagctg ccctcggggc
     1261 ggcgcaccaa gacggggttc tcggtcgact cggaagtgct ggaaaacatt cgccatcagc
     1321 acccaatcgt cgagctgatc ctggagcacc gcacgctggc caagctcaag tcgacctacg
     1381 tcgatgcctt gccgctgcag gtcaacgagc gcaccggccg ggtgcacacc tcgtacaatc
     1441 agacggtggc cgcgaccggc cgtctctcct cgaccaatcc caacctgcag aacattccga
     1501 tccgcaccga gttgggtcgt cgggtgcgac gcgccttcgt cgcagacacg cggccggact
     1561 ttcgactgtt cgacgattcg gttctcgtct ccgcggacta ttcgcaactg gagctgcggc
     1621 tcctggctca cttgagcgaa gagccgttcc tgatcgatgc tttcgcccgc ggggacgata
     1681 ttcataccgc gactgcggcg gtgatccacg gcgttccgcg tggtgacgtt acgcccgaca
     1741 tgcgccgggt cgccaaaacg gtcaatttcg gcatcatcta cggtatgcag gcccatgggt
     1801 tatcgcggga taccggcctc tcgcgtcagg aggcccagcg cttcatcgat cagtactgga
     1861 cgaacctacc ccaggtcaaa cgctactttg acggcaccct cgccttcggc attaaacatg
     1921 gctacgtcga gtcgatctac gggcgtcgcc gcatccttgg cgatctcacc tcggccaact
     1981 acacccgccg cgccgcagcc gaacggatgg cggtcaacat gccgttgcag gggagcgcct
     2041 ctgacatcat gaaactggcc atgatcaagc tcgatcgaga gctcgctcgc tcgggattac
     2101 gcgcgaagct gctcctccag gtccatgacg aattggtgct cgaagtcgag cgcgccgacg
     2161 ttccggctgt cgccgatctc ctgatgcaga cgatggagcg cgccgtcgac ctgcgagtgc
     2221 cgctggagac ggaggtccgg gcgggaccga attgggacga cctggcgccg atagaggccc
     2281 tcgcggtcag tgcgtagcat catcccggta gagatgtttg ccgtccggcc gacgctcgga
     2341 actgtcaaaa atgagacgtt ctgacggcaa acgggttcac tgcaacgaac tccgactcct
     2401 atgtacaatc ccggggtaac gtaaagtaat gaacgattca ggagagcgct ccgaacgatg
     2461 cccacatata cctatcgttg cgacacatgc ggtcacggtt tcgaggccgt gcaacggttc
     2521 gccgacgaac cgctcaagga ctgcccggaa tgcggtgctc cgatccggcg ggtcattcaa
     2581 cccgtgggag tcgtcttcaa aggctccggc tggtacatca cagatagccg tcccaagagt
     2641 tctagcgaca gcggggatgg tgcgagcaaa acggacaagt cagacaagtc ggagaagcgc
     2701 gacagcgcca agaccgacaa gacgccggcc aaggtggccg actaacgggt gaacgcgaat
     2761 gctttctcga cgcgtgccgc tctgtttctg gcagcctgag cactgcggcc gtggctgcgg
     2821 aggtcgttgc tcctgtccgt aggggcgcgc ccggcgcgtc ccgcaccgac gccgtcacgc
     2881 cgcgtgccgg aatgatcgct ccggccaccc cctgacagaa agggcgcgtc atgttgttcg
     2941 ctaaggaaat tcgagctatc cgtgagcgtg atccggcttc tcgctcggca atcgaggccg
     3001 cgcttacatc accgggcctg cacgccatcg ccctccatcg cctctcgcac tggttgtggc
     3061 aaaaagggct gttccttccc gcccgcctcc tctcgcagct cagtcggttc atcaccggca
     3121 tcgagattca tcccggggcc agaatcggtc aaggcgtctt catcgatcac ggcatgggcg
     3181 tcgtcatcgg tgagacggcc tcggtcggga ataatgtcac gatgtaccag ggcgtcactc
     3241 tcggcggaac ggggaaggag cgcggcaagc gccacccgac ggtggagtgc gatgccgtca
     3301 tcggcgtcgg tgcctcggtg ctcggtgcgg tcacgatcgg tgagggtgcc cgcgttggcg
     3361 ccggttcagt tgtgttgaag gacgtcccgc cgcataccac cgcagtcggc ctgcccgcac
     3421 gtgccgtctc ctggcgcgat ccgcactccg gtgagacccg gcgactcgag catt
//
LOCUS       DATAOZ010000087         3885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4244685, whole genome shotgun sequence.
ACCESSION   DATAOZ010000087 DATAOZ010000000
VERSION     DATAOZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4244685"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VHR64_02485"
     CDS             <1..1010
                     /locus_tag="VHR64_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3301719.1"
                     /translation="VATGAAAGLALGTAACVAGMDVTAMDRLPDTTGLRNEVIVQRGH
                     RNSYDHAIRSVGVRFVEVGYLGYPGAGGTMPWQIEAAITERTAAIACPILDTPGCQPL
                     PVVAEIAHRHGLPVIVDAAAELPPRSNLRRFIEEGADLVVFSGGKAIGGPQASGILAG
                     RADLIASVALQHQDMDVRPETWNKRDLLDSGLAGIPHQGFGRAMKVGREEIAGLITAL
                     RRFAAGSDEADFHSWSGLLDQIEHELREIPGIACDRTGPPRSKKPLPLLQITLTNAEP
                     GRAYAVINELLSGDPAIALGESYADQNILIVNPHGLTETEAALVGKQLRDVLGRTVDS
                     R"
     gene            1111..1917
                     /locus_tag="VHR64_02490"
     CDS             1111..1917
                     /locus_tag="VHR64_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX3301720.1"
                     /translation="MYIRPASGKKVMWQEILRSELEATLDQRPVVIVPIGSIEQHGPH
                     CPHDVDISVPFHLAIAAADAIDDFPVIVAPPISYGFTHYNMGEIGTITLGLETFIAVL
                     NDVARSIWANGFRRIIMLNGHGGNEQPNWSAAVKIAEDDVWVLALTYWHMADPELAEW
                     SEADAGSIGHGGEWETSLQLHLREELVDMNRAVHDPWRRKFSDELSRFAHFPERRREM
                     EHGVMGNPLVASKEKGERLFNVLLERLIATCREYHEIDPPRYREFGSHCP"
     gene            2061..2375
                     /locus_tag="VHR64_02495"
     CDS             2061..2375
                     /locus_tag="VHR64_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301721.1"
                     /translation="MFAKIVRWRLTPEIQGEQAFDQFIRDLSRRNTPILRDHGLLDGF
                     VVRIAPDMMLSMNLYDSDADAESAWRDVIANLGNELDGKLEFLERYVGPAVDMPMLLD
                     EA"
     gene            complement(2431..3171)
                     /locus_tag="VHR64_02500"
     CDS             complement(2431..3171)
                     /locus_tag="VHR64_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3301722.1"
                     /translation="MDQSIERFVLVTGAAGRIGRSFVAGTASRCRFRLADREVGELDD
                     AAGYEIVPLDVADLDGCRAACAGIDTVVHLAADPSPEADFAGSLLENNIRGAYNIFRA
                     AADAGCRRVIYASSLHVVAGYSIEEAAGPDSPVRPLTMYGVSKCFGEATARYFAEAEG
                     LSCIAVRIGAFEAPWIREEPTREVLSGFISARDLNQLLVRCIEAPAISFAILHGISNN
                     RIKRVSLTETESLLGYGPRDDGFAWVEG"
     gene            3342..>3885
                     /locus_tag="VHR64_02505"
     CDS             3342..>3885
                     /locus_tag="VHR64_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301723.1"
                     /translation="MIGAVGICLALTLGLTVHASLAQQSSAKQAKVILAELNESGVSG
                     VASLVAKGDQTQVSMSVIGVLGDNPTHIHQGTCKDLDPNPQYPLTNIVLPSSTLTGVS
                     DTLVDAPLSDLLATPHLILIHKSQKDIGTYLACGNIVPGALTTEEQAGANGEAPLPNT
                     GIGSWTDGSPASRALLLALVA"
BASE COUNT          750 a         1270 c         1201 g          664 t
ORIGIN      
        1 atgtcgcgac cggcgcggcc gccgggctgg ctctcggcac cgcggcgtgc gtggccggca
       61 tggatgtcac ggcgatggat cgcctgccgg atacaaccgg tctgcgcaac gaggtcatcg
      121 tccagcgcgg gcaccgcaac agctacgacc acgcgatccg gtcggtcggc gtccgtttcg
      181 tcgaggtcgg ctaccttggc tatcccggcg cgggcgggac gatgccatgg cagatcgagg
      241 cggccatcac cgagcggacc gccgctatcg cctgcccgat cctcgatacg ccgggctgcc
      301 agccgctgcc agtagtggcc gagatcgcac atcgccacgg tttgccggtg atcgtcgatg
      361 ccgcggccga actgccgccg cgctccaacc tgcgccgctt tatagaagaa ggtgccgacc
      421 tcgtcgtctt ctcaggcggc aaggcaatcg gcggaccgca ggcaagcgga atcctggccg
      481 gccgggccga tctgatcgca tcggtcgccc tccagcacca ggacatggat gtccggcccg
      541 agacctggaa caagcgcgac ctgctcgaca gtggtttggc cggcatcccg caccaggggt
      601 tcggccgggc aatgaaggtc ggccgggagg agatcgccgg gctgatcacg gccctgcggc
      661 gattcgcggc cggttccgac gaggccgatt tccactcgtg gtccggcctg ctcgaccaga
      721 tcgagcacga gttgcgcgag atcccgggca tcgcctgcga ccgcaccggc ccgcccagat
      781 caaagaagcc cctaccgctc ctgcagatca cgctaaccaa cgccgagccc ggcagagcct
      841 atgccgtgat caacgaactg ctatccggcg acccagcgat cgccctcggc gagtcctacg
      901 cagaccaaaa catcctgatc gtcaacccgc acggcctcac tgagacagaa gcggccctcg
      961 tcggcaaaca actacgtgac gtgcttgggc ggacagtgga cagtcgatag acgacagaaa
     1021 gtcaggagcg aaaacagaac tgaccactga ccactgtcca ctgtcctctg tgttctgtcg
     1081 actaatcact aacccctcag gagggagttc atgtacattc gtccggcgtc cggcaagaag
     1141 gtcatgtggc aggagatcct gcgcagcgag cttgaggcga cgctcgatca gcggccggtc
     1201 gtgatcgtgc caattggatc gatcgagcag cacgggccgc actgcccgca cgacgtcgat
     1261 atttccgtac ccttccatct cgcgatcgcc gcggccgacg cgatcgatga ctttccggtc
     1321 atcgtcgcgc cgccgatttc gtatggtttc acccactaca acatgggcga gatcggcacg
     1381 atcaccctcg gtctcgagac cttcatcgcg gtcctgaatg acgtcgcccg cagcatctgg
     1441 gctaatggtt tccggcggat catcatgctc aacggccatg gcggcaatga gcagccgaac
     1501 tggtcggcgg cggtcaagat cgccgaggac gatgtctggg tcctggccct cacctactgg
     1561 cacatggccg atcccgagct ggccgagtgg agcgaggcgg acgccggcag catcggtcac
     1621 ggcggcgaat gggagacgag tctccagctc catctgcgcg aggaactggt cgacatgaac
     1681 cgcgcggtcc acgacccgtg gcggcgcaag ttcagcgacg agctaagccg gttcgcccac
     1741 ttcccggagc ggcggcggga gatggagcac ggcgtgatgg gcaacccgct ggtcgccagc
     1801 aaggagaagg gtgagcggct cttcaacgtc ctgctcgagc ggctgatcgc cacctgccgc
     1861 gaataccacg agatcgatcc gcctcgctac cgtgaattcg gcagccactg cccgtgacgg
     1921 tggagggtaa ttggtcgttg gtagatggag aaccaacgac caacgaccat ctaccaacaa
     1981 ctcacgactt ctacggatat cattcgcacg ccgacaatgc ggacggttga tattgggtat
     2041 gggatgaggc agggcgggaa atgttcgcca agatcgtgcg atggcgcctg acgcccgaaa
     2101 ttcagggtga gcaggcattt gatcagttta ttcgtgacct ttcgcgacgc aacacaccaa
     2161 tcctgcgcga tcatggactg ctcgacgggt tcgtggttcg gatcgctccc gacatgatgc
     2221 tatccatgaa tctctacgat tccgacgccg acgccgaatc ggcctggaga gacgtgatcg
     2281 ccaacctcgg caatgagctc gatgggaaac tcgagttcct cgagcgctac gtcggcccgg
     2341 cagtcgatat gccgatgctc ctcgacgagg cctgacgttt cgctgagtga tggcggatgg
     2401 caggtgggtc tctatccgcc accgaagcgc tcaaccttcg acccacgcga agccgtcgtc
     2461 ccggggcccg tatccgagca acgactcggt ctcggtcagg ctgacgcgct tgatgcggtt
     2521 gttcgagatg ccgtggagga tcgcgaagct gatcgccggg gcttcgatgc agcggaccag
     2581 gagctggttc aggtcccgcg cgctgatgaa acccgacagc acctctcgcg tcggctcctc
     2641 ccggatccac ggtgcctcga atgcaccgat tcggaccgcg atgcacgaca gtccctcggc
     2701 ctcggcgaaa tagcgtgccg tcgcctcgcc aaagcacttg ctgacgccgt acatggtcag
     2761 cggccgcacg ggagaatcag gcccggccgc ctcttcgatt gaatatcccg caacgacatg
     2821 caggctactg gcatagatca cccggcgaca gcccgcatcg gcggctgcgc ggaagatgtt
     2881 gtacgcgccg cggatgttgt tctcgagcag agaccccgcg aaatccgcct cgggtgatgg
     2941 atcggcagcg agatggacga cggtgtcgat gccggcacag gccgctcggc acccgtccag
     3001 atcggccacg tccagcggga cgatctcgta cccggcggca tcatctaatt cgccaacctc
     3061 tcgatcggcc aggcgaaagc gacaccggct cgcggtcccc gccacgaacg agcggccgat
     3121 tcgtccggcc gcaccggtca cgagcacgaa tcgctcaatc gactggtcca tcgctccctt
     3181 gttgctcatg ttcacgaaat gtggccaaaa cgactgaatc tgcgccgaat ccagatacta
     3241 tgtcaccaat cgtcaaaccc atacgatcgg tgaactggtg caactggccg gaggacccca
     3301 tggcagcctt gcgatctgtg gtttcggacc gtgcgtttcg gttgatcggc gcggtgggca
     3361 tttgcctggc gctcaccctg ggactcaccg tgcacgcctc gctcgcccaa cagagctcgg
     3421 cgaaacaggc gaaggtcatc cttgccgagc tgaacgaatc aggggtctcc ggcgtcgcga
     3481 gcctggtggc caagggagac caaacccagg tcagcatgag cgtaatcggt gtcctgggcg
     3541 acaacccgac gcatatccac caggggacct gcaaggacct cgaccccaac ccgcaatatc
     3601 cgctcaccaa tattgttctg ccctcatcga ccttgaccgg cgtcagcgac acactggttg
     3661 atgcgccgct gtcggacctg ctggcaacgc cgcacctgat cctgatccac aagtcgcaaa
     3721 aggatatcgg cacctatctc gcctgcggca acatcgtgcc cggtgcgctg acgactgagg
     3781 aacaggcggg tgcgaatggc gaggcgccgc tcccgaatac cggcatcgga agctggaccg
     3841 atggatcgcc ggcgagccgc gcgctgctgc tggccttggt cgccg
//
LOCUS       DATAOZ010000088         3460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9245655, whole genome shotgun sequence.
ACCESSION   DATAOZ010000088 DATAOZ010000000
VERSION     DATAOZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9245655"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1810)
                     /locus_tag="VHR64_02510"
     CDS             complement(<1..1810)
                     /locus_tag="VHR64_02510"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEX3301724.1"
                     /translation="MQKTTQPILSATARGWAIIAVLGLLIGMVPLNATAQDTPTTNVV
                     DPIWSLVPGEATAAISINADPESSQWQTAAGLLHAAGLEDVVYGAINEFIDQTDLAPA
                     NFVPGQSSILGGTIAAATWGDATDSFARGAVYISAPDPDRAYRSLAAQLITESNPDAV
                     EVDLPGGRATLDTNGNGTAAVMQLNDIVVVTESQDAQKIAALATGGGPTLADFDPFVR
                     VMSAQSGDLIARAYANGPASGQLLGVSLSQPFGLGYTEFGILFALVAPVSGTHASFGL
                     SASEDGFLLHTARLPAPLIGPELPGAGPSADLAAEVSGLSDLFLAGTDLGQSPIFTQL
                     IDTIVIGAASSSGWSPYEPTLDVRAWAYEQLKRMTNIDVQADLLNQLKGDYLIAGKVD
                     SVDAPSGVQLAVTSEIADAETVTNALAKLSAVLSLSTAGRSDITYENDPSERSGLTNT
                     ISVRDGDSGRAVLTIKYGLSGPGFVLTVNAGEELLNPTPGDDLATDPEYRAAIAKLPV
                     QDGSPIYLNLQSLITVANLTSLEPPDTWGIDNPRHASLDGIRSLVGMTFEQNDLAITD
                     LLLEIAVPATPVPPSEATPETTGMFAPDLVIRDAAAI"
     gene            complement(1884..2927)
                     /locus_tag="VHR64_02515"
     CDS             complement(1884..2927)
                     /locus_tag="VHR64_02515"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX3301725.1"
                     /translation="MSSITTQIPVIDCRGSAREIGRAHGESARDLIALGLGRWRESLA
                     AQRGDVDQYIADFLGRTSFTNAIQAYAPHILDELRGIAEGAGQPFELILAYNLMDEEW
                     SFRTDRLDDLAPGCTVVAVAGAAIGQTMDIPTIHDGTQIVLAVEPDHGPSQRLLSAAG
                     MLGLTGANSAGVGVVVNNLNQLPSSASGLPVQVLTRRLLEHETADEAARWTESVPHAV
                     GQHYLIADPATVISLEGHGEGVVRVPMADRYVHANHPLACAASRPDTDKIEFLSNTHV
                     RHDRAETMIGSATDQAALERILSDREAPISRERAKGFMTFGGISIGLTVPPTVHVTAG
                     PPHESEWINISWN"
     gene            3106..>3460
                     /locus_tag="VHR64_02520"
     CDS             3106..>3460
                     /locus_tag="VHR64_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301726.1"
                     /translation="MTATSPRSAAVPQADIDQLNMTLSVPAVTPDDAEYDEARKVFNA
                     MIDRKPAMIVHCADVADVIAAVNFARDHGMLLAVRGGRQYLARGGPRHARVRVGNADR
                     NHLDHWRRRSDARRGC"
BASE COUNT          573 a         1022 c         1259 g          606 t
ORIGIN      
        1 cgatcgcggc tgcgtcgcga atgacgagat cgggagcgaa catgccagtg gtttcgggcg
       61 tggcttcgga gggcggcacg ggcgtggccg gaacggcgat ctcgagcagc aggtcggtta
      121 tggcaaggtc gttttgctca aacgtcatgc cgacgagaga gcggatgcca tcgagcgagg
      181 cgtgccgcgg gttgtcgatg ccccaggtgt cgggtggctc gagcgaggtc aggttggcga
      241 cagtgatcag gctctggagg ttgaggtaga tcggcgagcc gtcctgaacc gggagcttgg
      301 caattgccgc ccggtactct gggtcggtag ccaggtcgtc gccgggagtc gggttgagga
      361 gctcctcgcc cgcgttgacc gtgagcacga agcccggccc gctcaggccg tacttgattg
      421 tcagcactgc gcgaccgctg tctccgtccc gaaccgagat ggtgttcgtg aggccggatc
      481 gctcggacgg gtcattctcg tacgtgatgt cggagcggcc ggcggtcgag agagagagaa
      541 ccgcgctcaa cttggcgagg gcattggtga ccgtctcggc gtcggctatt tccgacgtga
      601 ccgcaagctg gacacccgat ggcgcatcga ccgagtcgac cttgccggcg atcaggtagt
      661 cacccttgag ttggttcagc aggtcggcct gaacgtcgat gttggtcatg cgcttgagct
      721 gctcgtacgc ccaggcgcgc acgtcgagtg tgggctcgta cggtgaccag cctgacgacg
      781 aagctgcgcc gatcacgatc gtgtcgatga gctgggtgaa gatcgggctc tggccgagat
      841 cggtcccggc aaggaacagg tcgctgagtc cggagacctc cgcagcgagg tcggcggatg
      901 ggccggcgcc gggcagctct ggaccaatca ggggcgccgg cagtcgggcg gtatggagca
      961 ggaacccgtc ctcagatgcc gaaaggccga agctggcgtg cgtgccggag accggggcga
     1021 ccagcgcaaa gaggatgccg aactcggtgt agccgagccc gaacggctgc gacaacgaga
     1081 cgccgagcaa ctgaccggac gccgggccgt tggcgtaggc gcgagcgatc agatcgccgg
     1141 actgggcgct catgacacgg acgaaggggt caaaatcggc cagggtcggt ccgccgcccg
     1201 tggccagggc ggcgatcttc tgggcgtctt gcgattcggt gacgacgacg atgtcgttga
     1261 gctgcatgac cgcggccgtg ccgttgccgt tcgtatcgag cgtggcgcgg ccgccgggca
     1321 ggtcgacctc gaccgcatcg gggttggact cggtgatgag ctgcgctgcg agcgagcgat
     1381 aggcgcgatc gggatccggt gcggagatgt agacggctcc gcgcgcgaag gagtcggtcg
     1441 catcgcccca ggttgccgca gcgatcgtgc cgccgaggat cgacgattga ccgggaacga
     1501 agttggccgg agcaaggtcg gtctgatcta tgaattcgtt gatcgcgccg tagacgacat
     1561 cctccaggcc ggcggcgtgg agcaagccgg cagcggtctg ccactgcgac gattccggat
     1621 ccgcgttgat ggagatcgca gcagtcgctt ccccgggaac caggctccag attgggtcga
     1681 cgacgttggt cgtgggagtg tcctgggcgg tggcgttcag cgggaccata ccaatcagta
     1741 agccgagcac ggcgatgatc gcccagccgc gagcggtggc ggacaggatg ggttgggtgg
     1801 tcttctgcat cacggtctcc aggtcgaaat tggtcgcgcc cgactcgctc aatgtccctt
     1861 caggtgacgt tcggcacggc tgtttagttc caggagatgt tgatccactc cgattcgtgc
     1921 ggcgggccgg cggtgacatg cacggtcggc gggactgtaa ggccgatgct gatgccgccg
     1981 aaggtcatga agcccttcgc ccgctcgcga ctgatcggcg cctcgcgatc cgagaggatc
     2041 cgctcaagcg cggcctggtc cgtggctgag ccgatcattg tctcggcacg atcgtgccgg
     2101 acgtgcgtat tcgagaggaa ctcgatcttg tcggtgtcgg gccgggacgc ggcgcaggcc
     2161 agcgggtgat tggcatggac gtagcgatca gccatcggga cgcggacgac gccctcgccg
     2221 tgcccttcga ggctgatgac cgtggccggg tcggcgatca ggtagtgctg accgactgcg
     2281 tgcggaacgg actcggtcca gcgcgcggcc tcgtcggcgg tctcgtgctc gaggagccgg
     2341 cgggtcagga cctggacggg cagaccgctg gccgaggacg ggagctggtt caggttgttg
     2401 acgacgaccc cgactccggc gctgttggcc ccggtcaaac cgagcatccc ggcggcggag
     2461 agcaggcgct gggacggtcc gtgatccggt tcgaccgcga gcacaatctg ggtgccgtcg
     2521 tggatggtcg gaatgtccat ggtctggccg atcgccgctc cggccacggc gacgactgtg
     2581 cagccgggcg cgaggtcgtc gagccggtcg gtgcggaagc tccactcctc gtccatcagg
     2641 ttgtaggcga ggatcagctc gaacggctgg cccgcgcctt cggcgatgcc gcgcagctca
     2701 tcgaggatat gcggggcata cgcctggatc gcattcgtga atgatgtccg gccgagaaag
     2761 tcggcgatgt actgatcgac gtcgccgcgc tgggcggcga ggctctcgcg ccagcggccg
     2821 agaccgaggg cgatcaagtc acgcgccgat tcgccgtgcg cgcggccaat ctcgcgggcg
     2881 gagccgcgac agtcgataac cggaatctga gtggtgatgc tgctcataga tcgttcctcc
     2941 gtgtggcgac atggtagtcg ctccatcgtc tgccgtgggc tactgcatat tgcgttggcc
     3001 ggttagcatc gctaatattc atgctcatat cccacacccg gtttgcagtc cgttgacacg
     3061 cgtgcggcag gtcgcctgcg cgaagacccc gtcaggaggc aactgatgac tgctacatcc
     3121 ccccgttccg ccgccgttcc gcaagcggat atcgatcagc tgaacatgac gctcagcgtt
     3181 ccggcggtaa caccagacga tgcggagtac gacgaggcgc gcaaggtctt caatgcgatg
     3241 atcgaccgca aaccggcgat gatcgtccac tgcgccgacg tagcggacgt gatcgccgcg
     3301 gtcaattttg cgcgggatca cgggatgttg ctcgccgtcc gtggcgggcg gcagtacctg
     3361 gcacgaggtg gaccacgcca cgcacgcgtt cgggttggca acgccgaccg gaatcatctc
     3421 gaccactggc gtcggcggtc tgacgctcgg cgggggtgtc
//
LOCUS       DATAOZ010000089         4262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6352483, whole genome shotgun sequence.
ACCESSION   DATAOZ010000089 DATAOZ010000000
VERSION     DATAOZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6352483"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1708
                     /locus_tag="VHR64_02525"
     CDS             191..1708
                     /locus_tag="VHR64_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3301727.1"
                     /translation="MDEQRAFDVVVVGGGAAGCVMASRLSEDPERRVLLLEAGPDYGS
                     DPAAWPPALLDASGVCVDLHDWGYGQPKQNGDQMSLFRGRLMGGTSAINGSVWLRGAP
                     ADYDGWAAMGNPGWGFADLEPYFRMAENDPRDGTTGPVPISRLDGGGPNAVERALIDA
                     ARSSGIPWVDNENAAEPQDCCIGAAPRNVLGNVRMNGALTYLAAARNRPNLEIVPDTA
                     VDRVVFNGGHVTGVLTVDGRTFSGREVVLAAGAYGSPAILMRSGIGPAGHLREHAIPV
                     VCNLPGVGANLLDHPLAANGFCLHLVHPDVAPDRQAFIRLVIKGRSGQVADGVDYSLF
                     SMVNNDPDAGGWVFVLPTSLMIARSQGTVRLTSSDPNATLEINHNHFADPADLEAICD
                     GIELATRLAATPPLAEILTPSPASLTWSSRAELRELVLAHADTTYHPSSTCRMGPPDD
                     PWTVVDQAGRVRGVQGLRVADASIFPGSPRANIHATVVAVAEKLADEMRREYAQA"
     gene            1906..3084
                     /locus_tag="VHR64_02530"
     CDS             1906..3084
                     /locus_tag="VHR64_02530"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX3301728.1"
                     /translation="MSIIATLAGIGLIGLILVDAFETVVLPRRVTRRFRLTRLIVLNS
                     WRVWVRVARLLPSTGGHEGAGQREGFLGTFGPLSLLMLLGIWASGLVIGFGFLYWGTG
                     TPLGGPDPHPGLGPTLYLSGETFFTIGYGDLAPREGIGRFLAVAEGATGFSFLAVIIA
                     YLPVIFTAYSQRETRITLLDARAGSPPTAAAFLARQKTGPEPFDCDTYLREWEVWTAE
                     LLEIHLSLPILTTWRSQHDRQSWLGALTLILDTTALVIVGLETSAGRVPGRQARLTFA
                     MARHAVGDLCQILVAEPRAPVDDRLPAADLEQLREHLAAAGIALRPGQGAADQLIALR
                     QLYEPYVAALAARLQVAVPTWLPVADAQDDWETTAWQWDPAEARDPVRGSRDNGTLTG
                     "
     gene            3129..3824
                     /locus_tag="VHR64_02535"
     CDS             3129..3824
                     /locus_tag="VHR64_02535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3301729.1"
                     /translation="MAQSASAPTHGPAFLFDLDGTLVDSVYQHVLAWRQALEDVGIEL
                     AVWRIHRRIGMSGGLLVNALLRETGQAVDADVVARLQERHAAAYMELAGQVRPLPGSR
                     ELLETLSAYQVPWAIATSGRREVAGPVLETLGVPPSIPIVTRDEVPYAKPDPDLFLKA
                     AERLGVNASNSVVVGDSVWDLLAAQRARALGIGLLSGGYGRAELEAAGAYQVYEDPAE
                     LLRRIDEVGVRRA"
     gene            4025..>4262
                     /locus_tag="VHR64_02540"
     CDS             4025..>4262
                     /locus_tag="VHR64_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301730.1"
                     /translation="MEGDETSSRPRAERNVTISIGAIALEGSLVVPDPALGVVLFAHG
                     SGSGRHSPRNRYVAGVLQEGGIATLLIDLLTEDEE"
BASE COUNT          652 a         1311 c         1556 g          743 t
ORIGIN      
        1 gggcgagccg cccggcacta cgtttggagt gcgcagtgat ggggccggta cgggcgacaa
       61 tgacgggcag actcgaccct tcctcattga cagtattccg taggatggag agtgatcttc
      121 ctcttgcggc gccgatttcc ggtgaccgcg cggctcatct gaccgattta caagtggaac
      181 tggtgcgccc atggacgaac agcgcgcgtt tgacgtggtt gttgtcggcg gtggtgcggc
      241 gggctgcgtg atggcgtccc gcctgagcga ggatccggag cggcgcgtgc tgctgctcga
      301 ggcggggccg gattacgggt ccgatccggc cgcgtggccg ccggcattgc tcgatgcgag
      361 cggcgtctgc gtcgacctgc acgactgggg atacggccag ccgaagcaga acggcgatca
      421 gatgtcgttg ttccgaggcc ggctaatggg tgggacctcg gccatcaacg ggtcggtctg
      481 gctccgtggc gcgccggccg actatgacgg atgggcggcg atgggcaacc cgggctgggg
      541 atttgccgat ctggagccgt acttccgcat ggccgagaac gacccgcgtg acgggacgac
      601 cggcccggtg ccgatcagcc ggctcgatgg cggtgggccg aatgcggtcg agcgggcgct
      661 catcgatgcg gcgcgatcat ccggcatccc ctgggtcgac aacgagaacg ccgccgagcc
      721 gcaggattgc tgcatcggcg cggcgccgcg caatgtgctc ggtaacgtgc ggatgaacgg
      781 cgcgctgacg tacctcgccg ccgcgcgaaa tcgcccgaat ctcgagatcg tgccggacac
      841 agccgtcgat cgcgtcgtct tcaacggcgg acacgtaacc ggcgttctga ccgtggatgg
      901 gcggacgttc agcgggcgcg aggtcgtact ggcagccggc gcctatggct cgccggcgat
      961 tctgatgcgg tcggggatcg ggccggccgg tcacctccgg gagcatgcca ttccggtcgt
     1021 ctgcaacttg ccgggggtcg gggcgaatct gctcgatcat ccgctggcgg cgaacggctt
     1081 ttgcctgcac ctggtgcatc ccgacgtggc gccggaccgg caggcgttca tccggctggt
     1141 gatcaagggc cgcagcggac aggttgcgga tggggtcgac tactccctct tctcgatggt
     1201 caacaacgat ccggatgccg gcggctgggt cttcgtcctg ccgaccagcc tgatgatcgc
     1261 gcggtcgcag ggaacggtgc ggctgacctc gtccgacccg aacgcgacgc tggagatcaa
     1321 ccacaaccac ttcgccgatc cggccgatct ggaggcgatc tgcgatggga tcgaattggc
     1381 cacgcgactg gccgcgacgc cgccgctggc cgagatcctg acgccatcgc ccgcctcgct
     1441 gacgtggtcg agccgggcgg agctgcggga gctggtgctg gcccacgccg acacgaccta
     1501 tcacccgtcg agcacttgcc ggatggggcc gccggatgat ccgtggacgg tggtggatca
     1561 ggcggggcgg gtgcgtgggg tgcaggggtt gcgggtagcc gatgcctcga tcttcccggg
     1621 cagtccgcgg gcgaatatcc acgcgacggt ggtcgcggtc gccgagaagc tggccgacga
     1681 gatgcgccgg gaatacgcgc aggcgtagcg tgagccccac acaggttggc ccacttacag
     1741 cgtagcgcgg ggttaccatg ccccgcgcta cgtgaaggtg atacctgcga acgcgtgtct
     1801 cgtcaccacc atgccggcca cgggggccgg cgctacgatt tacaggcggt agatcgcacg
     1861 gccgcgatcg gcgcgctgat cgtgagattc ggtaggtggg tgagcgtgtc aattatcgcg
     1921 actttggctg ggattgggct gattgggctg atccttgtcg atgccttcga aacggtggtc
     1981 ctgccgcggc gggtcacccg gcgctttcgg ctaacccggc tgatcgtgct caacagctgg
     2041 cgggtctggg ttcgcgtagc ccggctcttg cccagcacgg gcggccacga aggggcggga
     2101 cagcgcgagg gattcctcgg tacctttgga cccttgtcgc tcctcatgct gttgggcatc
     2161 tgggcctccg ggctcgtcat cggcttcggg ttcctctact ggggaaccgg aacgcccctg
     2221 ggcggtccgg atccgcatcc cgggctaggc ccgacgctgt acctcagcgg cgagacgttc
     2281 ttcacgatcg gctatggcga tctggcgccc cgggagggga tcggccggtt cctggcagtc
     2341 gcggaagggg cgacgggatt cagcttcctg gccgtgatca tcgcctatct gccggtcatc
     2401 ttcaccgcct actcgcagcg ggagacacgg atcacgctgc tcgatgcccg agccggatcc
     2461 cctcctactg ccgcagcctt cctggctcgc cagaagacag gaccggagcc gttcgactgc
     2521 gacacgtacc tgagggagtg ggaagtctgg acggccgagt tgctcgaaat ccatctctcc
     2581 cttccgattc tcaccacgtg gcgctcgcag catgatcgcc agtcgtggct gggcgcgctg
     2641 accctgatcc tcgataccac cgcgctcgta atcgtgggtc ttgagacgag cgccggtcgc
     2701 gttcccgggc gccaggcccg attgacgttc gcgatggctc ggcacgcggt cggcgaccta
     2761 tgccagatcc tggttgctga gccccgggcg ccggtcgatg accggctgcc cgccgcagac
     2821 ctcgagcagc tgcgggagca cctggcggcg gcgggcatcg cattgcgccc gggtcaaggg
     2881 gcggccgacc aactgattgc cctgcggcag ctctacgagc cctatgtggc cgccctggct
     2941 gcacggcttc aggtcgcggt gcccacctgg ttgcccgtgg cggacgcaca ggacgactgg
     3001 gagacgaccg cctggcagtg ggatcccgca gaggcgcgag acccggtccg tggctcgcgc
     3061 gataacggca ccttgaccgg ctgatgatgg ttcgtggcta gtctcagtac cgcgaacggg
     3121 aggttcagat ggcgcaaagc gcttccgcac cgacgcacgg ccccgccttc ctttttgatc
     3181 tcgacggcac gctggttgac agcgtgtacc agcatgttct ggcctggcgg caggcgctgg
     3241 aggacgtggg catcgagctc gcggtttggc gaatccatcg ccgaatcggg atgagcggcg
     3301 ggctcctggt caatgcgctc ctgcgggaaa ccggtcaggc ggtcgatgcg gatgtcgtag
     3361 cccggctgca ggagcggcat gcggcggcgt acatggagct cgccggtcag gtgcggccgc
     3421 tgcccgggtc gcgggagtta ctggagacgc tttcggcgta tcaggtgccc tgggcaatcg
     3481 ccaccagcgg gcgccgggag gtggcgggcc cggtgctgga gacgctgggg gtaccgccgt
     3541 cgattccaat tgtgacgcgg gacgaggtgc cgtatgccaa gcctgatccc gatctcttcc
     3601 tgaaagccgc agagcgactc ggcgtcaatg cgagcaactc ggtcgtcgtg ggcgatagtg
     3661 tctgggatct gttggccgcg cagcgagcgc gggcgctcgg gatcgggcta ctctccggcg
     3721 ggtacgggag ggcagagctc gaggcggccg gggcgtacca ggtctacgag gatccggccg
     3781 agctcctgcg ccggatcgat gaagtcggcg tgcggagggc ctgaacagtt gagggttgag
     3841 ggaacgaaat gccgccttgg tctggggccg gcgctcgatg ggattgcgag cgctcatgcg
     3901 atgttcgtcg caagggttcg tccgaaatcg ctcccattaa gatggggtgg gacacctcat
     3961 accgaatacg caatgaaacc tgtttgcttc gggacggcga tggaccagga ggcgatgcag
     4021 agcgatggag ggggacgaga ccagcagtcg acccagggca gagcgcaatg tcacgatctc
     4081 gatcggcgcg attgcgctgg agggaagcct ggtcgtgccg gatccggcgc tgggcgtcgt
     4141 cctgttcgcg catgggagcg gaagcgggcg gcatagcccg cggaaccggt acgtcgcggg
     4201 cgtgctccag gagggcggca tcgcgacctt gctgatcgac ctgctgaccg aggacgagga
     4261 gc
//
LOCUS       DATAOZ010000090         6817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3195161, whole genome shotgun sequence.
ACCESSION   DATAOZ010000090 DATAOZ010000000
VERSION     DATAOZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3195161"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VHR64_02545"
     CDS             <1..389
                     /locus_tag="VHR64_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX3301731.1"
                     /translation="KARVRLVKGAYAEPSSIAYPGTTEVDDNYLRLMYLLLEHGNYPA
                     IATHDMKLIHAAREFATDHAIPADRFEFQMLFGVRRDAQTSLVADGYRMRVYVPFGTE
                     WYPYFTRRIAERPANALFVLRQVADR"
     gene            444..1529
                     /locus_tag="VHR64_02550"
     CDS             444..1529
                     /locus_tag="VHR64_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301732.1"
                     /translation="MPALAVIGGALLVSVILINRSSDAGFDPAVRANPVVDLLGWLPA
                     TDQSRRAYAAWVEQPGAPLDLTDAVDRLSITPAPLALGRSSEWQRTTGISASQVTGWA
                     SAPGAGVTVLSGTFDAAGIQSRLDQAGYRSTTYRSVPIWLRPDPIEQGTIVEGDDMMA
                     SNAIAVYQDRVVFGWDEQSVHRALDAASGVSASLATESAVDTANITTGLSGLMIVDQR
                     DLAIECGVGGGWLKSDFSRPSGRAVAVLYHMDDAGTPVTSIWANYDDSDTAMALLPVL
                     ETDWRSGYANQIGLGGRVSDYATVQSVRQIDAWVVADLTAGRDNRWVRSGIRYLVAIC
                     EQASPLIPAGAPNRATPVASPSPMEAP"
     gene            1526..2029
                     /locus_tag="VHR64_02555"
     CDS             1526..2029
                     /locus_tag="VHR64_02555"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX3301733.1"
                     /translation="MNFRASLRSSDMFVSLQRLSRVAQDHPIVGPYLEMVTRLPMYAR
                     LGADLARDPDVPATAKASLVAAGAYAISPIDLVPGIIPVAGQMDDLAALLIAIRFAVR
                     MTPKEVAYPHLERAGITTQQIDDDLKSVRDTTIWLARQVANGVRSLATNGATRVSANL
                     RRWTARE"
     gene            2292..2591
                     /locus_tag="VHR64_02560"
     CDS             2292..2591
                     /locus_tag="VHR64_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301734.1"
                     /translation="MAFRTHSPAPANDEFVAETINPGCPMLIRAAADHPTKNHALMMR
                     CALGWSLHDELDYARCGATHAVQDCWRVHPELTPVVALPGSDESLEVEALQHVAD"
     gene            2749..4047
                     /locus_tag="VHR64_02565"
     CDS             2749..4047
                     /locus_tag="VHR64_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX3301735.1"
                     /translation="MLFATPLMTAAPISVSAQTEEATPVAEEQPPVDQATPDVTPSPE
                     ATEPTVDLPTEEASPVDGTPIADPTQDATATPEATATETPDFNSAASLTDIQITLKCT
                     TSPETVRVTNTGTDPIDLTKFGTFKDVIDGEPFTISRTLKPGTTAIFQSGAGAQYGTV
                     LTQSYLFTNSAYDSEGVQIETSVGTAVKMCDPKPAPPPPPLGKLSDLKVTLSCSTDPE
                     TIRVTNNGTGWITLKGIATYIDPIADEPFAIMPRRVLKPGQTAIYQSGTGAKYGTILT
                     KEFIFTNSAYEKDGVRISTDVGKLYKACPAKPVPPERWIEVNLSAQYLTAWVGNTKVN
                     GTLVSTGRPGFDTPTGTFYILYRYRYDTMAGCIQGECYYVPDVPWTQYFTNYGHALHG
                     AYWHNNFGQTMSHGCVNLPLWFAEWLWNWATYGTRVWIHY"
     gene            complement(4140..4586)
                     /locus_tag="VHR64_02570"
     CDS             complement(4140..4586)
                     /locus_tag="VHR64_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301736.1"
                     /translation="MPLLRKKRNPGQPAWFDLEAPAEKTVPNGWRFSLKGSPRHYDDV
                     KKIIEADPGARLYFSEALTYERGAGVALWRIEANSFAWLKPLYAWWAEMERVEPIRFT
                     FFLYLPNDPKHAELDLRQHTPEEVERFVRENAPRSEAEAQKAARRF"
     gene            4719..5267
                     /locus_tag="VHR64_02575"
     CDS             4719..5267
                     /locus_tag="VHR64_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEX3301737.1"
                     /translation="MPHNIEIKLACTPGQSAEIQRRLNERQLSATNRLGQIDTYFRVP
                     HGRLKLREIDAACEHSAELIQYHRPDEPGARTSTYRRIPVDPEQAESLKAALCDALGE
                     LVTVRKQRTVAIWRSTRIHLDQVEGLGQFVELETVLGNAPEGDAVGRVEFDDVVGWLG
                     LASLEAIPGSYSDLLIAKGQPA"
     gene            5264..6184
                     /locus_tag="VHR64_02580"
     CDS             5264..6184
                     /locus_tag="VHR64_02580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3301738.1"
                     /translation="MKVGVIIPLHAPQTGPNVFAPTYAQVREVALTAEREGLDSAWIT
                     DHLIFRMPPDFVNRASHEAFTIWAGIAEATSRIELGAMVLCTAFRNPAVMAKEAVSLD
                     AVAGHRIILGVGCGWHEPEFKAFGIPFTNRVSMFEEAMSIIHPLLREGNVTFHGKYYQ
                     AEDCVLVPPDDRPGGIPILIASKQPRMMELTARFADQWNTAWWGDVDGYREAAAKQDA
                     ACERIGRDPSTLVKTAGVMIDFFEEVPPADSYDPARVVKGPLEHVAGKLRTYADAGCA
                     HAICFLVGLTPEKTEMLAEASRLAGLTAAV"
     gene            complement(6449..6670)
                     /locus_tag="VHR64_02585"
     CDS             complement(6449..6670)
                     /locus_tag="VHR64_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301739.1"
                     /translation="MQTAATFASPDEERPPLCPKCNKRLVILASQSARDENGGYVRQQ
                     LWGCPRGHATAVRRGGTFQPVEVLTELVG"
BASE COUNT         1222 a         2226 c         2149 g         1220 t
ORIGIN      
        1 gaaaggcgcg ggtccggctc gtcaagggcg cctatgccga gccgtcgagt atcgcctatc
       61 cgggcacgac cgaggttgac gacaattacc tccgcctcat gtatctgcta ctggagcacg
      121 gtaactaccc ggcgatcgcc acccacgaca tgaaattgat ccacgccgca cgagaattcg
      181 cgaccgatca tgcgatcccg gccgaccgct tcgaattcca gatgctcttt ggcgtccgcc
      241 gggacgccca gacctcactc gtggcggatg gctatcgcat gcgcgtctat gtccccttcg
      301 gaaccgagtg gtacccgtac ttcacgcgcc ggatcgccga gcggccggcc aacgcgttgt
      361 tcgtgctccg ccaggtcgcc gaccggtagc gctcagggat gagattgccg aagataccgg
      421 aacggtggcg acaccggcgg ctcatgccgg cgcttgcggt catcggcgga gcattgctgg
      481 tcagcgtgat cctgatcaac cggtcgtccg atgccggttt cgatcctgcc gtgcgcgcca
      541 acccggtcgt ggatctgctt ggttggctgc cggcgaccga tcaatctcgc cgggcctacg
      601 cggcttgggt ggagcagccc ggcgctccgc tcgacctgac cgacgcagta gaccgtctct
      661 cgatcactcc cgctccgctt gccctcggcc gcagcagcga atggcagcgc accaccggga
      721 tctcggcctc gcaggtgact ggctgggcat cggcgccggg tgcgggcgtg acggtcctct
      781 cgggcacatt cgacgcggcc gggattcagt cgcggctcga tcaggccggc taccggtcga
      841 cgacctaccg ctcagtgccg atctggctga ggccagaccc gatcgagcag ggaacgatcg
      901 tcgaaggcga cgatatgatg gcgtccaatg cgatcgccgt ttaccaggac cgcgtggttt
      961 tcggctggga tgagcaatca gtgcaccgcg cgcttgacgc cgcgtccggt gtttcggcct
     1021 cgctagcaac ggaatcagcc gtcgacacgg cgaacatcac gaccggcctg tccggactca
     1081 tgatcgtcga tcagcgggac ctcgcgatcg agtgcggggt tggcggcggc tggctgaaaa
     1141 gtgacttttc tagaccctcc ggccgcgcag tggcggtcct ctatcacatg gacgatgcgg
     1201 ggacaccggt gacgtcgatc tgggccaact acgatgacag cgatacggcg atggcgcttc
     1261 tgccagtcct ggaaacggac tggcgatctg gctacgcgaa tcagatcggc ctcggcgggc
     1321 gcgtctccga ctatgcgact gtccagtcgg tgcgccagat tgatgcctgg gtagttgccg
     1381 acttgacggc cggtcgcgac aatagatggg tacgctccgg catacggtat ctggtagcga
     1441 tctgcgagca ggcctcacca ctgattccgg ccggcgcgcc aaaccgggct acgccggtgg
     1501 cgagcccatc gccgatggag gcgccatgaa cttccgagcc agtctccgat ccagtgacat
     1561 gttcgtgtcg ctccagcgcc tgagccgcgt cgcccaggat cacccgatcg ttggcccgta
     1621 tctcgagatg gtgacccgat tgccgatgta tgcccgcctc ggggccgatc tcgcccggga
     1681 cccggatgtg ccggccacag ccaaggcatc gctcgtcgcg gccggcgcat acgcaatttc
     1741 gccgattgac cttgtgccgg gcatcatccc ggtggcaggg cagatggacg atcttgcggc
     1801 gctgctgatc gcgatccggt ttgccgtgcg gatgacccct aaggaggtcg cataccccca
     1861 ccttgagcgg gccggcatca cgacccagca gatcgacgat gacctgaagt ccgtccgcga
     1921 cacaacgatc tggttggcaa ggcaggtggc aaatggtgtg cgatcgttgg cgacaaacgg
     1981 tgcgacccgc gtcagtgcca atttgcgcag gtggacggcc cgcgagtaat cgccggccga
     2041 cgaagagccg cgcgctcccc tatactcgga tcgacggcgc aactgcgccg tcatgtatcc
     2101 cgatatctgg cacactgcgc agcttttggc tcggacgcac tgatccgcag gacgcgtcca
     2161 cgaatgcttc gatgatcgaa tgggtccgct ttcggccagg agatgaaccc gtatctcgcc
     2221 tgactcagat gccctgacga aaccggtcca gtaaaccgca gcgtgccatt gcccggggaa
     2281 aggcttgcgc aatggcattt cgcacacatt cgcctgctcc ggcgaacgat gagttcgtgg
     2341 cggagacaat caatcccggc tgcccgatgc tgatccgggc cgccgcagat catccgacga
     2401 agaatcacgc gctgatgatg cgttgcgcgc ttggttggtc gctccatgac gagctcgatt
     2461 acgctcgctg tggggccacg cacgccgtcc aggattgctg gcgcgtgcac cctgagctga
     2521 ccccggtcgt cgcgctgccg ggctcggatg agtcgctcga ggtggaggca ctgcaacacg
     2581 tagcggatta agctattgac cctccccgct ctggtcaata ggatgaaaca aacacccgca
     2641 gagtggcgta ttaatagata gaactgggtt ggcggccaag tcggttggtt tggggagggt
     2701 tcgatgcgtc gtctagggtc tgtgggcttt gcatggctca tagctttcgt gctgtttgcc
     2761 acgccgttga tgacggctgc cccaatcagc gtttccgcac aaactgaaga ggcgaccccg
     2821 gttgccgagg aacagccgcc ggttgatcag gcgacgcccg acgtgacgcc gtctccggag
     2881 gccaccgagc cgacggtcga tctcccgacc gaagaggcat caccggtcga tggcacgccc
     2941 atcgccgatc ccacccagga tgcgacggcg actccagaag ccacggccac cgagacaccg
     3001 gatttcaact ccgccgcgtc cctgaccgac atccagatca cgctcaagtg cacgacgtcg
     3061 cccgagacgg tgcgcgtcac caacaccggt accgatccca tcgatctgac caagttcggc
     3121 accttcaagg acgtcatcga cggcgagccg ttcacgatca gccggacgct caagccgggg
     3181 acgaccgcga tattccagtc cggcgccggc gcgcagtacg gcacggtctt gacgcagagt
     3241 tacctgttta ccaacagcgc ctacgacagc gagggcgtcc agatcgagac gtcggtcggc
     3301 accgctgtca agatgtgcga tccgaaaccg gccccacctc cgccgccgct gggcaagctg
     3361 agcgacctga aagtcacgtt gagttgctcg acggatccgg agacgatccg cgtcacgaac
     3421 aacggcaccg gctggatcac gctcaagggc atcgcgacct acatcgaccc cattgccgac
     3481 gagccgttcg cgatcatgcc ccggcgggtg ctgaagcctg gccagacggc catctaccag
     3541 tccggtaccg gcgccaagta cggcacgatc ctgaccaagg agttcatctt cacgaactcc
     3601 gcctatgaga aggacggcgt gcgcatctcg acggacgtcg gcaaactcta caaggcctgc
     3661 cccgccaagc cggttccgcc ggaacggtgg atcgaggtca acctcagcgc gcagtacctg
     3721 accgcctggg tcggcaacac gaaggtcaac ggcacgctgg tcagcaccgg ccgacccggt
     3781 ttcgacacgc cgaccggaac cttctacatc ctgtatcgct accggtatga cacgatggcc
     3841 ggctgcatcc agggcgagtg ctattacgtg ccggatgtgc cgtggacgca gtacttcacg
     3901 aactacggcc atgccttgca cggggcgtac tggcacaaca acttcggcca aaccatgagt
     3961 catggctgcg tgaatctgcc gctctggttc gcggagtggc tctggaactg ggccacgtac
     4021 ggcaccaggg tctggatcca ctactagcca ggaacctaac gggccgggga gcaatccccg
     4081 gcccgttcgc atttcagcgg tatggtgatc ggcgcgtacg cacttccggc tggacagagc
     4141 tagaagcggc gagcggcttt ctgagcttcg gcctcggagc ggggcgcgtt ttcgcggacg
     4201 aagcgctcga cctcttccgg ggtgtgctgg cgcaaatcca gctcggcgtg cttggggtcg
     4261 ttcggcaggt agaggaagaa ggtgaaccgg atcggctcga cccgctccat ctcggcccac
     4321 caggcgtaga gcggcttgag ccaagcgaag ctgttcgcct cgattcgcca aagcgcgacc
     4381 cccgcgccgc gctcgtaggt cagcgcctca ctgaagtaga ggcgcgcgcc cggatcggcc
     4441 tcgatgatct tcttgacgtc gtcatagtga cgcggcgagc ccttgagcga gaaacgccac
     4501 ccgttcggca ccgtcttttc ggcgggcgcc tccaggtcga accaggccgg ctggccagga
     4561 ttcctcttct tcctgagaag cggcatttcc gtcaatcctc cgtccaccag acaggtacag
     4621 catgcgccaa tttcacccgc cgattgtact cgaccgcgcc ccggcgtcgg tgatgcagcg
     4681 ccgcttcgga tagactgccg ggagagggga gccgggcagt gccgcataac atcgagatca
     4741 agcttgcctg cacgcccggg caatcggcgg aaatccagcg gcgcctgaac gagcgccagc
     4801 tttctgccac caaccgtctc ggccagatcg atacctactt ccgcgtgcca catggccggt
     4861 tgaagcttcg ggagatcgat gcggcctgcg agcatagcgc cgagttgatc cagtaccacc
     4921 gcccggacga gcccggcgcc cggaccagca cctaccgccg gattccggtc gacccggaac
     4981 aggccgagtc cctgaaggcc gcgctctgcg acgcgcttgg cgaattggtg accgttcgca
     5041 agcaacgtac ggtcgcgatc tggcgttcga ctcgcatcca cctggatcag gtcgagggcc
     5101 tcggccagtt cgtggagctg gagacggtcc tcggcaacgc ccctgagggt gatgcagtcg
     5161 ggcgtgtcga gttcgatgac gttgtcggtt ggttgggatt ggcgagcctg gaggcgattc
     5221 ccggctcata cagcgatttg ctgattgcga aaggacagcc tgcatgaagg tcggcgtcat
     5281 cattccgctc cacgcgcccc agacgggtcc gaacgtgttc gcgcccacgt acgcgcaagt
     5341 tcgggaggtc gccctgacag ccgagcgcga gggacttgat tcggcctgga tcaccgacca
     5401 cctgatcttc cgcatgccgc cggatttcgt caatcgcgct agtcacgagg ccttcacgat
     5461 ctgggccggc atcgccgagg caacgtcacg gattgagctc ggcgcaatgg tcctctgcac
     5521 cgccttccgg aacccggcgg tgatggccaa agaggcggtc tcgctcgatg ccgtcgccgg
     5581 tcaccggatc atcctcggcg tcggctgcgg ctggcatgag ccggagttca aggcgttcgg
     5641 cattcccttc acgaaccggg ttagcatgtt cgaggaggca atgtcgatca tccatcccct
     5701 gctccgcgag ggcaacgtca cgttccatgg caagtactac caggcggagg attgcgtcct
     5761 cgtgccgccg gatgaccggc cgggcgggat cccgatcctg atcgcgtcca agcagccgcg
     5821 catgatggag ctgaccgctc gctttgccga ccagtggaac acggcctggt ggggcgatgt
     5881 cgacggctac cgggaggccg ccgcaaagca ggatgctgcg tgtgagcgca tcggtcgcga
     5941 tccctccacc ctggtcaaga ccgccggcgt catgatcgac ttcttcgaag aggtcccacc
     6001 agcggattcc tacgatccgg cgcgagtcgt caaggggccg ctcgagcatg ttgccgggaa
     6061 actccgaacc tatgccgatg ccggctgcgc acacgcgatc tgcttcctgg tcggtctgac
     6121 gccggagaag accgagatgc ttgccgaagc ctcccgcctc gccggcctca ccgcggccgt
     6181 ttagccgaag cggaacgcga aaaatgacgt ggcgcggggt taccacaccc cgcttgatgc
     6241 atccccacga acggttgatg gagctgggcg ggtaactcgg ttggttcccc cgacaatgag
     6301 gccgtcgtaa cgctcgggcg gggcatggta accccgcgct acgtccttac ccgcaggcga
     6361 ccattggcgt ggcattccat gaaacatcga gcccggcggg aagtatccgg ccgggctccc
     6421 actactctat tcgattgacc cgaggcgatt agccgaccag ctccgtcagg acttcgacgg
     6481 gctggaatgt cccgccccgc cggactgccg tggcatgacc gcgtgggcac ccccagagct
     6541 gctgccgcac atacccgccg ttctcatccc gcgccgattg cgacgcgaga atgacgagcc
     6601 gcttgttgca cttcgggcag agcggcggtc gctcctcgtc ggggctggca aacgttgctg
     6661 cggtttgcac gggcatacct ggttgttcta ttgacaagtc gctcacctga agcggttttc
     6721 cttctgttca cgatcgcgcg gcgggcaggc gcggagctca tggcactcgg tgcggcggtc
     6781 tgattccaga cgtgccgccc ttgccgacat atcgtgc
//
LOCUS       DATAOZ010000091         5106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7930844, whole genome shotgun sequence.
ACCESSION   DATAOZ010000091 DATAOZ010000000
VERSION     DATAOZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7930844"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VHR64_02590"
     CDS             <1..273
                     /locus_tag="VHR64_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301740.1"
                     /translation="DRRVFVDRLDFVTAAGNVDRVVTPLCIFRTVDGRLMVESRHTWA
                     GPDMVKEATGFAVDVDESTPVTPEIRTDEREALLRIDPVGVSLTEF"
     gene            441..659
                     /locus_tag="VHR64_02595"
     CDS             441..659
                     /locus_tag="VHR64_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301741.1"
                     /translation="MLADASFDAYELPFSRFVAEALIARDGPQEIADRADRLGLPPVA
                     RDVAVAACVGTACDCSLIQLEDAAGASL"
     gene            831..1115
                     /gene="rpsP"
                     /locus_tag="VHR64_02600"
     CDS             831..1115
                     /gene="rpsP"
                     /locus_tag="VHR64_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX3301742.1"
                     /translation="MLKIRLRRMGAKKRPSYRIVVAESTAPRDGRFIEIVGLYDPLTE
                     PATVRLNEERARHWLSVGAQPTDTVRDILRKANLVPDDPRLAARGKQNAA"
     gene            1253..1573
                     /locus_tag="VHR64_02605"
     CDS             1253..1573
                     /locus_tag="VHR64_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX3301743.1"
                     /translation="MDSEDQVTMDAENEDSMDGDNQVITSAETDLAELVRYVATKLVH
                     NPDDISVESELRAGTVYVSLRVPEEEMGRVIGREGRTAKAIRTLVSIAGTRHNVHARL
                     DIDG"
     gene            1548..2168
                     /gene="rimM"
                     /locus_tag="VHR64_02610"
     CDS             1548..2168
                     /gene="rimM"
                     /locus_tag="VHR64_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX3301744.1"
                     /translation="MPDSTSTGNTRQPQKRRRFTPPNLARTRGPAPDTPLDDVQLAVG
                     RVVGTHGLDGELRVQLLTDNPEHLLEAEQLLVGDQRTPYTIDSIRFHKGLALIAFDEI
                     VDVDAAETLRGSVLRISGSDLPPLAENEYYLYQVVGIEARTEAGEVIGTVSDVIETGA
                     NNVFVVTPADGGKELLFPSIPDVVTDIRPTDGYLVIRPQTWWDDQP"
     gene            complement(2676..3455)
                     /locus_tag="VHR64_02615"
     CDS             complement(2676..3455)
                     /locus_tag="VHR64_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEX3301745.1"
                     /translation="MPSIAVLVAMEAELIHLRNFATSSSHRTIGVWDAYDLSINGHEV
                     LTVRSGIGMLNAAASTERVVAEFKPEIILNYGCAGAHRRDIMPGDVIIGSETVNHGAL
                     NILRDGTEVHNDRGTIVSGERIFPAVIDADPKLLETAQSVASELTIEPWPEGVVWPSS
                     VPYRNPQVHVGPLASSEIWTQSLDRLDVLHSRHGSLSEDNESGAFAHVAYIHEIPFLS
                     IKDIANNEYHAASDLAEYSDFPTAEVGKRAASLVAALITAL"
     gene            complement(3469..3819)
                     /locus_tag="VHR64_02620"
     CDS             complement(3469..3819)
                     /locus_tag="VHR64_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX3301746.1"
                     /translation="MQHAANGGMYHDVAAVLRNGFRIAAGLMITGIVLALIRQEPLST
                     KVDPITDIPNALLHLHSSAFIDLAIISIMLTPLASVIAILRSFVISADRRFAAYTAGV
                     LIILSASIALSLLR"
     gene            complement(3819..4664)
                     /locus_tag="VHR64_02625"
     CDS             complement(3819..4664)
                     /locus_tag="VHR64_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3301747.1"
                     /translation="MDVSQSIELGVAASITAILGSMLGLGGGVFLVPIFTLFFGVDQK
                     LAIGASAVAVATNSVVGSTVHLESRFTNLRFAMMMEVTTAIGALVGAIVGVWAPEQAI
                     NFIFASVLVYAAVSMAIKRAGSNEIVSDGPDPHGLAGEYHDPAANRTVRYIPQRLRLG
                     LSMSTIAGVMSGMLGVGGGVIKVPVMNVLMRVPVKAAAGTSAFMVGITAVASSSVYYS
                     KGKIDPTIVVPAMIGIYLGSQFGSRLTRKLHAERLTLIFVLILAYLSLSLYVKAFGYT
                     LPGQQ"
     gene            complement(4745..>5106)
                     /locus_tag="VHR64_02630"
     CDS             complement(4745..>5106)
                     /locus_tag="VHR64_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301748.1"
                     /translation="TFEQSGGKGKPKFGQLTVCWAKSEAEGKKTALEIWPNAGIPGEL
                     GQELPMPAHFEQAAKLVTEETIAKQVVCGPDAERHVAEIQKYVDAGFDHIYIHQVGPD
                     QEGFMKFYKQDVLPRFN"
BASE COUNT          958 a         1573 c         1618 g          957 t
ORIGIN      
        1 gatcggcgcg tcttcgtcga ccggctggac ttcgtgaccg cggccggcaa tgtggaccgg
       61 gtggtcactc cgctgtgcat cttccgcacg gtcgatggcc gcctcatggt tgagtcgcgg
      121 catacgtggg caggtccgga catggtcaaa gaagcgaccg ggttcgcggt tgacgtcgac
      181 gaatcgaccc cagtgacgcc agaaattcgc acggatgagc gagaggccct gctccgaatc
      241 gatccagttg gcgtttcctt gacagaattc tgaggttatt ccgggtaact tttatcaaac
      301 tgttgccata ttgggtttcg gctggtaggt tggttcgggt ggcgtgctac tgcgctgggc
      361 tagcgccacc cggacgtatt agctgatctg acttcgggtc acacccgggg tgagatccat
      421 agggaaggga ccgtcgagac atgctggctg atgcctcgtt cgatgcttat gaactgccgt
      481 tcagtcgatt cgtcgccgag gcgctcatag ccagagatgg cccccaggaa attgccgacc
      541 gagccgaccg gctcggactc cccccggttg cgcgagatgt tgcggttgcg gcgtgtgtcg
      601 gcacggcgtg cgactgctcg ctgatccaac tggaagatgc cgctggggct tctctctaga
      661 tattgaattc cggcaccgga aggtgcgata ctggatggcg ctgcccgcga ggggagccgt
      721 tccgcgtgtc caattctcct ccatgttggg ccgccgctga acatctgacc aagacggttc
      781 gaacatgatg accggccgaa tgcggccggg atccgctgag gagtgattgc gtgctgaaga
      841 ttcgtttgcg tcggatgggc gccaagaagc gcccaagcta ccggattgtg gttgccgaga
      901 gcaccgcacc gcgcgacggc cggttcatcg agatcgttgg tctctatgac ccgctgactg
      961 agccggccac ggttcggctg aacgaggagc gcgccaggca ctggctgagc gtcggtgctc
     1021 agccgaccga caccgtgcgg gatatcctcc gcaaggcgaa cctggtgccg gacgatccgc
     1081 gcctggctgc ccgcggcaag cagaacgccg cttgagacgt gaccccggat ccgcggatgg
     1141 tgggccgggt cctgatatgc tgaaagctcg gcggggtgat ctgattcgct gatcccccgc
     1201 cgttttgttt accgccattt tcgacgccga aagcggctag ccggagcacg gtatggattc
     1261 cgaagatcag gtcaccatgg acgcggaaaa cgaggactcc atggatggcg acaaccaggt
     1321 catcacgtcc gccgagaccg atctcgcgga gctggtgcgc tacgtcgcaa ccaaactcgt
     1381 ccacaatccg gacgacattt cggtggagtc ggaacttcgc gctggcacag tctatgtgag
     1441 ccttcgggta cccgaagagg agatgggccg cgttattggt cgcgaaggcc ggaccgccaa
     1501 ggcaattcgc acgcttgtct cgatcgccgg aacccgccac aacgtccatg cccgactcga
     1561 catcgacggg taacacccgg cagccccaaa agcgacggcg cttcaccccg ccgaacctgg
     1621 cccggacgcg aggtccggcg ccggacacgc cgcttgatga cgttcagctc gcagtcggcc
     1681 gcgttgtcgg cacccacggg ctggatggcg agctgcgggt ccagctcctg accgacaacc
     1741 ctgagcacct gctggaggcc gaacaactcc tggtcggcga ccagcggacc ccgtacacca
     1801 ttgattcgat ccggttccac aagggcttgg cgttaatcgc cttcgacgag atcgtcgacg
     1861 tcgacgcggc cgagactttg cgcggcagcg tccttcgcat ctccggctcc gatcttccgc
     1921 cattggccga gaacgagtac tacctctacc aggtggtcgg catcgaagcg cggaccgaag
     1981 caggcgaggt gatcggaacc gtcagcgacg tgatcgagac gggcgccaac aacgtctttg
     2041 tggtcacgcc ggccgatgga ggcaaggagc tgctcttccc gagcatcccg gacgtcgtga
     2101 ccgacatccg accgaccgat ggttacctcg tcatccgtcc ccagacctgg tgggacgacc
     2161 agccgtagca cggcccgctc ctgagcggca aggctcttcc gtcaatgaga tccggattgg
     2221 cgatttcgct ccatagatga tcggatgcaa tgggtgcgtg gaaaagtgcg ccccttaggc
     2281 ccctatgaca tctatggtga acataaaccg ggtactggag ataatatctg cgcaagtctt
     2341 ggcagaatgc cattgctcgg atgcataggc gtcatagatg ccatagatga tcgttttgca
     2401 acgagccaac ccatacatga gcatctatgg cgcagggctg aagcggagtg caggagactt
     2461 gtccaatcgg tggcgtagcg cgaggttacc acgccccgct tgatgcatcc ccagatacgg
     2521 atgatggcgc cagggctgta tcagaatctg tcccccatcc agattgaccg gcaccgggtc
     2581 cggtcggggc gtggtcctct ctccggctct ctcctcaagg gaagagggca tccccgcgcc
     2641 acggcaccga ccggacaacc cttctgaact tcgcgtcaga gcgcggtgat caaggccgcg
     2701 acgagactgg ctgcgcgttt gccgacttcc gcggtcggga agtcgctgta ctcagccagg
     2761 tcgctggcgg cgtgatactc gttgttcgcg atgtccttga tcgagaggaa cgggatctcg
     2821 tggatgtagg cgacgtgcgc gaaggcgcct gattcgttgt cctccgacag gctgccgtgc
     2881 cgcgaatgca ggacatccag gcgatcgaga ctctgtgtcc agatctcgga tgaggccagc
     2941 ggtccgacat ggacctgcgg gttccggtac ggcaccgacg acggccagac gaccccctcc
     3001 ggccacggtt cgatcgtcaa ctcactcgcg accgattgcg ccgtctccag cagtttcggg
     3061 tcggcatcga tgaccgccgg gaagattcgc tcgccggaga cgatcgtgcc gcgatcgttg
     3121 tgcacctcgg tgccgtcgcg caggatgttc agcgcgccgt ggttgaccgt ctccgagccg
     3181 atgatgacgt cgcccggcat gatgtcgcgt cgatgagcgc cggcgcatcc gtaattcagg
     3241 atgatctccg gcttgaactc cgcgacaacc cgctcggtcg aggcggccgc gttcagcatc
     3301 ccgatcccgg atcgaacggt aagtacctcg tgcccattta ttgagaggtc gtaggcatcc
     3361 cagacgccga ttgtccggtg gctcgatgag gtcgcaaagt tccgaagatg gatcagctcc
     3421 gcttccatcg cgacgaggac tgcaatcgac ggcatgacga atgggctcct agcgtaacaa
     3481 tgagagtgcg atgcttgcgc tcaaaataat cagtacgccg gctgtgtagg cggcgaatcg
     3541 gcggtcggcg ctgatgacga aactgcgcag gatggcaatg accgaggcca gcggcgtcag
     3601 catgatgctg atgatcgcca ggtcgatgaa tgcgctgctg tgcagatgca gcagggcgtt
     3661 cgggatatcg gtgatcgggt cgaccttcgt cgagagcggc tcctgacgga tgagcgccaa
     3721 cacaatcccg gtgatcatca gaccggccgc aatccggaac ccgttgcgca ggacggccgc
     3781 cacatcgtga tacatgccgc cgtttgccgc gtgctgcatc actgttgacc cggcagggta
     3841 tagccgaacg ctttcacgta cagcgacagg ctcaggtagg cgaggatcag gacgaagatg
     3901 agcgtcagcc gctcggcatg aagcttgcgg gtcaggcgcg aaccgaactg cgaacccaga
     3961 tagatgccga tcatggccgg aactacgata gtcggatcga tcttcccctt cgagtagtag
     4021 accgaagacg aggcgacggc cgtgatccca accatgaagg cgctcgtgcc ggcggcggcc
     4081 ttgaccggca cgcgcatcag cacgttcatg accggcacct tgatcacgcc accgccgacg
     4141 cccagcatcc ccgacattac gccggcgatc gtgctcatcg agaggccgag tcgaagccgc
     4201 tgggggatat agcgcaccgt ccggttcgcg gcgggatcgt gatactcgcc ggccaggccg
     4261 tgcggatcgg gtccgtccga gacgatctca ttggagccgg ctcgcttgat tgccatcgag
     4321 acggcggcgt agacgagaac cgaggcgaag atgaagttga tcgcctgctc cggcgcccag
     4381 accccgacga tcgcgccgac cagcgcgccg atcgcggtcg tgacttccat catcatcgcg
     4441 aagcggagat tcgtgaaccg gctctcgagg tggacggtcg agccgacaac cgagttggtg
     4501 gccaccgcga ccgcgctcgc gccgatcgcg agtttctgat cgaccccgaa gaagagcgtg
     4561 aagatcggga cgaggaacac gccaccgccg agcccgagca tcgagccgag gatggccgtt
     4621 attgacgcgg cgacgccgag ctcaatcgat tgcgagacgt ccaaaccgcg cccttgcccc
     4681 ttcgctcaat tcaccggcga tgatcttagc cgatttgagt gtggccggtg ggccctcacc
     4741 cgaatcagtt gaatcgcggc agcacgtcct gcttgtagaa cttcatgaac ccttcctggt
     4801 ccgggccgac ctggtggatg tagatgtgat cgaagccggc gtcgacgtac ttctggatct
     4861 cggcgacatg ccgctcggca tcaggcccgc agacgacctg cttggcgatc gtctcctcgg
     4921 tgaccagctt ggccgcctgc tcgaagtggg ccggcatcgg cagttcctgg ccgagctcgc
     4981 ccggaatgcc cgcgttcggc cagatctcga gggcggtttt cttgccttcc gcctcgcttt
     5041 tcgcccagca gaccgtcaac tgcccgaact tcggcttgcc ctttcccccg gattgctcga
     5101 acgttt
//
LOCUS       DATAOZ010000092         5465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_302754, whole genome shotgun sequence.
ACCESSION   DATAOZ010000092 DATAOZ010000000
VERSION     DATAOZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302754"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VHR64_02635"
     CDS             <1..1248
                     /locus_tag="VHR64_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3301749.1"
                     /translation="HSQDELAMIDQSRATATRPLESSPNDGRVIAALCVASFLAALNF
                     FGTAPFYPDIARDLDTTVPLVGQVTTMMILISTILGLAVGPIADRYGYRLMLVVGVIA
                     IGVNLIGIGLAPSYFVMLGLSICGGLADALVFGLPLAIAGIRFTGAAQKKAMGWTIGS
                     LSSASIIGTPILTTIGSVVGWRGALVGAGTVTMLAAWFVRASLPEDHHQAGTSLRVRD
                     IASAYLPVIRHRPTLRLYVASALRSVTWIGFITYLGAFLAEGLDLGTRQVGLVYMMSG
                     IGYAIGSVLSARVSVLSPRTTFSIATLIGAAVIAAVLVISVIPVSVSMVVVGSLASAF
                     AGLALATILVNESPAGTGTTMVLNGSMINLGASVGALIGGILISAGGYRALGVGLPVF
                     SVAAAILALWPAHAKDAVQPTTA"
     gene            complement(1245..2951)
                     /locus_tag="VHR64_02640"
     CDS             complement(1245..2951)
                     /locus_tag="VHR64_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX3301750.1"
                     /translation="MPANITGLFPRPSDYPDIRTTWKSDLIAGITVGVVALPLALAFG
                     ITTGLGAASGLVTAVVAGLVAAIFGGSHVQVSGPTGAMTVVLVPIVAHYGVDAVFAVS
                     IMAGIMIVLAGVLRLGRYLEYMPWPVFEGFTVGIAIIIFLQQVPNALGVEKPDGERTV
                     VVAARAVRDAVDHGSPAALLTVILVAAIMVGVVRLRAGLPGSLIAVLAATVLAEVMNL
                     DIGRIGSLPNSLPAPAFPSTSMSEMQALISAAFAVAALAAIESLLSAKVADGMADTQR
                     HNPDRELFGQGLANIVSPLFGGMPATGAIARTAVNVRSGARSRLASIIHALVLLVVVY
                     FGTGLVSRIPLAALAGVLAVTAFRMAERHNIMAVVRSTRSDAAVLVLTAACTVAFDLI
                     VAVEVGVAAAAFLALRHVAINSEAQTESLSSHMEIGPEDEARLLHEHIVVYRLDGALF
                     FGAAQRFLTILTRASDIRVVILRMSHIQVLDATGAKALGEIVTELQDRGITVLIKGVR
                     PQHMKILRAVGTVGRLTHERHLFATMPDAIEHARVHVRRWQETSNAPGGASALAPGVA
                     EA"
     gene            3154..3831
                     /locus_tag="VHR64_02645"
     CDS             3154..3831
                     /locus_tag="VHR64_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3301751.1"
                     /translation="MHILVVEDEMRLVTLLRRSLAEEGHAVDFVTNGEDALVWLESEP
                     GFDAVILDVMLPGIDGYETCLRLRNSGAQIPILMLTARDSVPDRVAGLDAGADDYLVK
                     PFAFAELAARLRALVRRSPETAETILSVADIRLDPARRQVWRGESEIHLSNKEFRILE
                     YLMRNSNRVLTRAMIADNVWDYDFPNVTNVIDVHIRWLRHKLDDPYPGRVLQTVRGVG
                     YRLIGET"
     gene            3828..5210
                     /locus_tag="VHR64_02650"
     CDS             3828..5210
                     /locus_tag="VHR64_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3301752.1"
                     /translation="MSARLPIRWRLTIWYAAFLAGSMILLGAGFYLGTRTLLFGFFEE
                     QLEKQSALAQSSVHSEGGVLTIDPNTVANLQDDEHFVRLLYLDGSAVVDTISSVGPAS
                     IDPELATAAASGESNVGSAHTPEGTILIKSTPVRAGSAIVGVLQVGASRGDVEDILRV
                     LTIGLMIAAPVVLLIAAGGGYLLAGRALAPVAAITDLAGSIGPKDLGRRLALDLPDDE
                     LGQLSRTFDAMLARIEDAFDRQRRFTGDAAHELRTPISFMRSQVDLALHRPRTAEEYQ
                     EALQEIDLDLERLTGLVNSLLTLARSDAGKLPVERTQIDLAELIGMIVEQFTPIATDV
                     GITLVNDATRTPVSIDSDLIVQLLVNLVENAIAHTPCGGTVTIGCRAESTGVRIRVED
                     SGPGIPPDDRERIFDRFYRVDTGRDRSRGGAGLGLSICRAIVEAHGGTIAVSESSLGG
                     ARFDVLLPMP"
BASE COUNT          962 a         1741 c         1769 g          993 t
ORIGIN      
        1 cattcgcaag acgagttggc aatgatcgac cagtcgcgcg caacggcaac tcgcccttta
       61 gagagctcac ccaatgacgg gagggtgatt gccgcgctct gcgtcgcatc atttctggcc
      121 gcgctcaact tcttcggcac cgcgccgttt taccccgaca tcgctcgcga cctcgacacg
      181 accgtgccgc tggtcggaca ggtcacgacg atgatgatcc tgatcagcac catccttggg
      241 ttggcggtcg ggccgattgc cgaccgatac ggctaccggc tgatgctggt tgtcggcgta
      301 attgcgatcg gtgtcaatct gattgggatc ggactggctc cgtcctactt tgtgatgctc
      361 ggattgagta tctgtggcgg cctggccgat gccttggtat tcgggctgcc gctcgcaatc
      421 gcaggaatcc ggttcaccgg cgcggcgcag aagaaggcga tgggttggac gatcggctca
      481 ctgagcagcg cgagcatcat cggcacgccg atcctgacta cgatcggctc ggtcgtcgga
      541 tggcggggag cgctcgtcgg cgccgggacc gtgacaatgc tggccgcgtg gttcgtgcgc
      601 gcgtcactgc cggaggacca ccaccaggcc gggacatcgc tccgcgtccg ggatatcgca
      661 tccgcatacc tgccggtaat acgccaccgc ccgacgcttc ggctctacgt ggccagcgct
      721 ctccgctctg tgacctggat tggatttatc acctatcttg gcgcattcct cgcggaaggg
      781 ttggatctgg gcacgcgcca ggtcgggctc gtctacatga tgtcaggaat cgggtacgcc
      841 atcggcagcg ttctcagcgc gcgcgtttcc gtcctctctc ctcgcaccac cttctccatc
      901 gcgaccctga ttggcgcggc cgttatcgcc gcggtactgg tcatctctgt catcccggtg
      961 tcggtgtcga tggtcgtggt cggcagcctg gcgtcggcgt ttgcgggtct tgccctcgcc
     1021 acaatccttg tcaacgagag tcccgcgggc accggcacca cgatggtgct gaacggctcg
     1081 atgatcaatc tcggcgcctc ggtcggcgcg ctgatcggcg ggattctgat cagcgccggc
     1141 ggatatcgcg cgctcggtgt cgggctgccg gtgttctcgg tcgccgccgc gattctggcc
     1201 ctctggccgg cgcacgcgaa ggacgcagtg cagccgacta ccgcctaggc ttcagccacc
     1261 ccgggagcaa gggccgacgc tccgcccggt gcgttgcttg tttcctgcca gcggcgcacg
     1321 tggactcgag cgtgctcgat cgcgtcgggc atcgtcgcga acagatgacg ttcatgcgtc
     1381 agtcgcccga ccgttccgac cgcgcgcagg atcttcatgt gctgcggtcg gacgcccttg
     1441 atcagcaccg tgatgccgcg atcctgcagc tcggtgacga tctcgccgag tgccttggcg
     1501 ccggtcgcat ccagcacctg gatgtgcgac atgcgcagga tcacgacgcg gatatcgctg
     1561 gcccgggtca ggatcgtcag gaatcgctgg gccgcaccga agaagagggc accatccagc
     1621 cggtagacga cgatgtgctc gtgcaagagc ctcgcctcgt cctcagggcc aatctccata
     1681 tggctgctga gcgattccgt ctgcgcctca ctgttgatcg cgacatgccg gagggcaagg
     1741 aacgcggcgg cggcgacgcc gacctcgacc gcgacgatca ggtcgaatgc aacggtgcac
     1801 gccgccgtca gcaccagcac cgcggcatcg gagcgggtcg agcgcacaac cgccatgatg
     1861 ttgtgccgct cagccatgcg gaacgcggtc accgccagga cacccgccag cgcggccaac
     1921 gggatccgcg acaccagtcc ggtaccgaaa tagaccacga ccagcaacac gagcgcatgg
     1981 atgatcgatg cgagtcgcga tcgggcgcct gagcggacat tgaccgccgt gcgagcgatc
     2041 gcgccggttg ccggcatgcc gccgaacaag ggcgagacga tattggccaa gccctgacca
     2101 aacaactcac gatcggggtt atgtcgctgc gtatcagcca tgccgtcggc gaccttggcc
     2161 gatagcaggc tctcgatcgc ggccagtgcc gcaacggcga acgcggccga gatcagcgcc
     2221 tgcatctcgg acatcgacgt cgaaggaaaa gcaggggccg ggagggagtt cgggagcgaa
     2281 ccaatccggc cgatatcgag gttcatgacc tcggccagca cagtcgcggc caggacggca
     2341 atcaacgagc ctggcaggcc ggcccggagt cgcacgacgc ccaccatgat cgccgcgacc
     2401 aggatgacgg tcagtagcgc cgcggggctt ccgtggtcga cggcatcgcg cacggccctg
     2461 gcggcgacga caaccgtacg ctcaccgtcg ggcttctcca ctccgagcgc gttgggtacc
     2521 tgctgcagga agatgatgat cgcgattccg accgtgaagc cctcgaaaac cggccacggc
     2581 atgtactcga gataccggcc gaggcgcagt acaccggcaa ggacgatcat gatgccggcc
     2641 atgatactga ccgcgaagac ggcatcgacg ccgtaatgcg ccacgatcgg caccagcacg
     2701 acggtcatcg cgcccgttgg tcccgagacc tggacgtgtg aaccgccgaa gatggccgcg
     2761 accagaccgg ccacgactgc cgtgacgagg ccactcgcgg cgccaaggcc ggtcgtgatg
     2821 ccgaacgcca gggcgagcgg caaggcgacg accccgactg tgatgccggc gataaggtca
     2881 ctcttccagg tggtgcggat gtcagggtag tcggagggtc ggggaaacaa gccagtgatg
     2941 ttggcaggca ctcgcacgtt cggtgatcac actttcgcct aaatcggagg gcaggacgcc
     3001 agggccggcg accccgttat cgccggaggc attacccgta ttggtagcat attttctggc
     3061 caccgacacg atcggatttg gcacgttggt ccttgattga cgataaatga cgcattaatg
     3121 aaaccttcat ctcggcagtt gagcatgtga aacatgcata tcctggttgt cgaggatgaa
     3181 atgcggctcg tcacccttct ccggcgcagt ctggcggagg agggtcacgc cgtcgacttc
     3241 gtgaccaacg gcgaagacgc gctggtttgg ctggaatctg agcccggatt cgatgcggtc
     3301 atcctcgacg tgatgcttcc cgggatcgac gggtacgaaa cctgcctccg cctcaggaat
     3361 tcaggcgccc agatcccgat cctgatgctg accgcgcgcg actctgtgcc ggatcgcgtg
     3421 gccggcctcg acgccggcgc ggacgactac ctggtaaaac cattcgcatt cgccgaactg
     3481 gcggcgcgcc tgcgggcact cgttagacgg tcgccagaaa ctgccgaaac gatcctgtcg
     3541 gttgccgata tccggcttga tcccgccagg cgacaggtct ggcggggtga gagcgagatc
     3601 cacctctcca acaaagagtt ccggattctc gagtacctga tgcgcaattc gaatcgggtg
     3661 ttgacgcgcg cgatgatcgc cgacaacgtc tgggattacg acttcccgaa cgtgacgaac
     3721 gtgattgacg tccatattcg ctggctgcgg cacaagctcg acgaccccta ccccggaagg
     3781 gtcctgcaaa ccgtgcgggg cgtcggctat cgcctgatcg gtgaaacgtg agcgcgcgcc
     3841 tgcccattcg ctggcggctg acgatttggt atgccgcctt cctggccggc tcgatgattt
     3901 tgctcggcgc cggtttctac ctcggcaccc gaacgctgct gttcggcttc ttcgaggaac
     3961 agctcgagaa acagtccgcg ctcgcccaga gctcggtcca ctccgagggc ggcgtcctga
     4021 ccatcgatcc caacaccgtc gccaacctgc aggacgacga gcacttcgtg cgcctcctgt
     4081 acctcgatgg ctcagcggtg gtcgacacga tctcgagcgt cggcccggca tcgatcgatc
     4141 cggagctcgc cacggctgcg gcaagcggcg aatcgaatgt cggctctgcc cacacgcccg
     4201 aaggcacgat cctgatcaag tccacgccgg tccgagcggg gtccgcgatc gttggcgtac
     4261 tgcaagttgg tgcctctcgc ggcgatgtcg aagatattct gcgggtcctg acgatcgggc
     4321 tgatgatcgc cgcgccggtc gttctgctga tcgctgcggg cggtggatac ctgctggccg
     4381 gccgcgcgct ggcgccggtc gcggcgatta ccgacctggc tggaagcatc ggtccgaagg
     4441 atctcgggcg ccggcttgcc ctcgacttgc cggatgacga gctcgggcag ctgtcgcgca
     4501 ctttcgatgc gatgctcgcc cggatcgagg atgcgttcga tcgccagcgc cggtttaccg
     4561 gcgacgccgc ccacgagttg cggacgccga tttccttcat gcgcagccag gtcgatctcg
     4621 cgttgcaccg gccaaggacg gccgaggagt accaggaagc gttgcaagag atcgatctcg
     4681 acctggagcg tttgaccggg ctggtcaact cgcttctgac cctcgcccgg tccgatgcgg
     4741 gcaaattgcc ggtcgagcgg actcagatcg acctggcaga gttgatcggg atgatcgtcg
     4801 agcagttcac gccgatcgcc accgacgtgg gcatcacgct ggtcaatgat gcgaccagga
     4861 ccccggtcag catcgacagc gatttgattg ttcaactcct ggtgaacctg gtcgaaaacg
     4921 caatcgccca caccccgtgc ggcggcacgg ttacgatagg ttgtcgagcc gagagtacgg
     4981 gtgtccgcat cagggtcgaa gattccgggc ccggcatccc gccggacgac cgggagcgaa
     5041 tcttcgaccg cttctaccgc gtggatacgg gacgcgatcg atcgagaggg ggggcaggtc
     5101 tcggcctgtc aatctgccgg gcaatcgtcg aggcgcatgg cgggacaatc gcagtcagtg
     5161 aatccagcct cggcggcgcc cgcttcgacg tgctgctacc gatgccttaa cggcaagtta
     5221 acgatgccgc tccacagtct gctcgccggg ctcgaatgtg gcccgggccg agtccgtggt
     5281 ctgtggacgt tacagggaga gaggggatcg catatgaaca ttcgatggag aggattgctc
     5341 gcgttggccg tggtgctcct ggtcattgcc ggcggcgtgc ttcgcgtcag cgcgaactcg
     5401 gccgggatat ccaaggggac tcagcctgtc tcgatggtgc atgccgtcga atcgggcgcg
     5461 cactc
//
LOCUS       DATAOZ010000093         6943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4774412, whole genome shotgun sequence.
ACCESSION   DATAOZ010000093 DATAOZ010000000
VERSION     DATAOZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4774412"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..767)
                     /locus_tag="VHR64_02655"
     CDS             complement(201..767)
                     /locus_tag="VHR64_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301753.1"
                     /translation="MAFRPASIVRIAIANDYVAHRARVAPRQWHWTEIVAIPIAAISM
                     LTFALTIAAEPWVRNCDPAAIRPIALASLLLLIAADLLFAPRRAWSRTRDNAAMNTVS
                     VAAALTLLFCVLSANAFAWPMLYPMLIFPLSLVLLPPIAIGFASRIPIGLEIRRWAVL
                     SCVVLAAALLLFGQHSYQAHIACRWPGV"
     gene            complement(780..2231)
                     /gene="pyk"
                     /locus_tag="VHR64_02660"
     CDS             complement(780..2231)
                     /gene="pyk"
                     /locus_tag="VHR64_02660"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX3301754.1"
                     /translation="MSLERRVKIVATLGPAVAGKDKLVDLISAGADMVRINAAHGSEE
                     TRVRLIEDVRAAADEVGKPVPILFDLRGLKIRTGPLPDGSGPVPFARGSDVRIVPMPE
                     STREGVIGINLPQLLQVIKPGSRILIADGLIELLVERIEGESALCKVGRGGILHSKQG
                     VTLPGAAIVGGSLTPDDRIDIAFAVNHGVDYLGLSFINDASDLVLAKGVAGAHGHAVP
                     ALIAKIERPEALNNVGEIATQADGLMVARGDLGVQLPPERVPRAQKHIIQTCNTLGVP
                     VITATQMLESMITQPMATRAETSDVANAVWDGTDAVMLSAESAVGQYPIESVQTMARI
                     IAEAEREGPIRSPASSHPLPTHGDNSLALADAIAQAAMALADTAPVEHILVFTLTGNA
                     AKRLAKYRPKPPMIAVTSEESTARRLALVWGLRSVVVPVEEDPDTMFRLAGQAVIDAG
                     LGRTDEYVLIVGSLPMMRVAGRTNLLHVRRLGT"
     gene            complement(2287..2682)
                     /gene="rbsD"
                     /locus_tag="VHR64_02665"
     CDS             complement(2287..2682)
                     /gene="rbsD"
                     /locus_tag="VHR64_02665"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEX3301755.1"
                     /translation="MKKNGILNHRLSDVIARMGHTQRLVIADAGLPIPPGVERIDLAV
                     VPGLPGVLDLARAIAHELQVEGVVLADELLDRNQALPNEVRALFPGVELRTVPHEEFK
                     QMCEGAVAIVRSGECTPYANIMLISGVTF"
     gene            2919..3767
                     /locus_tag="VHR64_02670"
     CDS             2919..3767
                     /locus_tag="VHR64_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301756.1"
                     /translation="MTPPRAARSPFTLLTPIVIGVVVAGAAVIFLVFMVIPSRGQADP
                     DAQRPDAPAINAPGVSELPESKPLGHPINRINEPLFLDPTSTPEELAMVEPTKRPTKT
                     PTEEVVVEFSSTPRVIRTPTPTYEAPVIVEQPTEAWVPTQAPPPVQPPPQPATSTPQP
                     APTATSVPPTIAPQPTKTAAPPPPPPATATPIPLSPTAVPPTPTQPPIEPTATLVNAT
                     PTATVPVIEGPPVTETPTDVTATPDSTPDKTETPTATSTPKPKKKRTPTATPDVTASS
                     EATPAN"
     gene            3840..4844
                     /locus_tag="VHR64_02675"
     CDS             3840..4844
                     /locus_tag="VHR64_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3301757.1"
                     /translation="MASIDRVPLGQTGLVVPPIAVGCAELGNMPEAFDYGVSEEDALA
                     TLRAALDSPLYYIDTAAWYGNGESERRVGKVLRERGGLPEGAILETKAGRNPDGDDYR
                     PETVKARFERSLELLGVDRVHICFIHDAEWTRFEDVMAPGGVMEILQDYKRQGVIGAL
                     GIAAGPVELSMQYVQTGMLDLLLTHNRYTLLNRAADAVQKEAKRGGMGVVNAAPYGSG
                     MLAKGPDAYARYAYQSASDEMIEVTRTIQQICDRHNIPLAAAALQFSMLDPNVDVTIV
                     GMSKPERIQQTIDLATVEIPQAVWDELGTLPTFDEDPELNRWRASGKQPVFVAAMGDK
                     "
     gene            4872..6074
                     /locus_tag="VHR64_02680"
     CDS             4872..6074
                     /locus_tag="VHR64_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX3301758.1"
                     /translation="MADFDTLIAGGHVIDPARGLSGAADVAIKDGKIAAVEAGIDRSR
                     AAKVIDAAGQYVTPGLIDLHTHVYWGVTYWGIEPDPVAARSGVTTWLDVGSAGGYTFP
                     GFRRWIAEPAKSRVFALLNMSSIGLVAPTWELANIDYCDIDLGQMIVDRNRDIILGIK
                     ARIDSSTTRGTGIEPLKRARDLADKVELPLMVHIGAAPPTLAEIVEYLRPGDILTHCF
                     TGQPNKIVDDAGAVIPSIKDLHDRGLVLDIGHGAGSFSFETAEAMLQQGILPDVISTD
                     IHQLAIQGPCFDMPVTLSKFLNLGMTIEQVIACATTNAAKAIRKTDLGTLCVGSVADV
                     ATFRIDEGDFTFQDVAMNCRKGSKLLVNTMTMIDGDVLPVVDDLPLQPWAKLPAHQAG
                     KVIPVRQV"
     gene            6281..>6943
                     /locus_tag="VHR64_02685"
     CDS             6281..>6943
                     /locus_tag="VHR64_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301759.1"
                     /translation="MFDTGRAEVGVDRGRAVGWAVLVTLAVAVLSILVWQGVTADGAP
                     DPTVPGLHHRAIVLDAGVLVFREGLEAILVLAAISSSFVGAQSTFRRPVAVGSGAAIL
                     ATVATWFIVIAIIGALNAPALDIQAGTGLLAVVVLLVVMNWFFHRIYWTGWIAHHNRR
                     RRDLLASGGSGVLLGLALLGFTAVYREGFEIVLFLQTLRLQAGSGVVLQGVAIGLGFT
                     AAV"
BASE COUNT         1233 a         2161 c         2323 g         1226 t
ORIGIN      
        1 ccgccaggtg acgatattgg catgcacgat attgcccctt ctgcgcatca ccagatgacc
       61 gatcggttaa cagttttcag cgcatattat cagggacaat cgcgagtttg aatccttaat
      121 tgaccacgtt gcgaacccga atctggttcc ggagagggcg ccacattgtt caacctgttt
      181 gcgcgaaggg gtgagtggtg tcagacgccg ggccagcggc aggcgatgtg cgcctggtac
      241 gaatgctggc cgaaaagcag cagcgccgcg gccagcacga cacacgacag aaccgcccat
      301 cgccgaatct cgagcccgat cggaatccgg ctggcgaatc cgatcgcgat cggcgggagc
      361 agcacgaggc tgagcgggaa aatgagcatc gggtagagca ttggccaagc gaatgcattg
      421 gcgctgagca cgcagaacag cagcgtcagc gcggcagcga ctgagacggt gttcatggcc
      481 gcgttgtcgc gggtgcggct ccaggcgcgg cggggcgcaa agagcagatc ggccgctatc
      541 aggagcagga gcgaggcgag cgcgatcggt cgaatcgcgg ccgggtcgca gttgcggacc
      601 catggctcgg ccgcgatggt cagcgcaaag gtcagcattg agatggccgc gattgggatc
      661 gccacgatct ccgtccagtg ccattggcgc ggggcaaccc tggcccggtg cgccacgtaa
      721 tcatttgcta tcgcgatccg gacgatgctg gccggtctga acgccatggc gtcctcgcgt
      781 cacgttccca ggcggcgaac atggagcagg ttggtgcggc ctgccacccg catcattggc
      841 agagagccga cgatcaatac gtactcgtcg gtacggccga gaccggcgtc gatcacggct
      901 tgccctgcca gtctgaacat cgtatcaggg tcttcctcga caggcacgac aacggaacgc
      961 aatccccaga ccaatgccag ccgccgggcg gtcgattctt ccgacgtgac cgcgatcatg
     1021 ggcggcttcg ggcggtattt ggccagccgc ttggccgcgt tgcccgtcag cgtaaagacg
     1081 aggatgtgct cgaccggggc ggtatccgcc agcgccatcg cggcctgggc gatggcgtcg
     1141 gccagcgcga gcgagttgtc gccgtgcgtg ggaagtggat gagatgatgc tggcgagcgg
     1201 atcgggccct cgcgctcggc ctcggcgatg atccgtgcca tcgtctggac cgattcgatc
     1261 ggatattgcc cgacggcaga ctcggccgag agcatgacgg cgtccgtgcc gtcccagacc
     1321 gcgttggcga cgtcgctggt ctcggctcgc gtcgccatcg gctgggtgat catcgattcg
     1381 agcatctggg tggcggtgat gacgggcacg ccgagcgtat tgcaggtctg gatgatgtgc
     1441 ttctgggcgc gcggcacccg ctccggcggc aactgcacgc cgaggtcgcc ccgggccacc
     1501 atcaggccat cggcttgcgt cgctatctcg ccgacattgt tgagggcttc cggtcgctcg
     1561 atcttggcga tcaatgccgg tacagcatgg ccgtgggcac ccgccactcc cttggccagg
     1621 accagatcgc tggcgtcgtt gatgaagctg aggccgagat agtcgacacc gtggttgacc
     1681 gcgaaggcga tatcgatccg gtcgtccggg gtcaacgagc cgccgacgat tgcggcgccg
     1741 ggcagggtga cgccctgctt gctgtgcagg atgccgccgc gtccgacctt gcagagcgcg
     1801 gactcgcctt cgatccgctc gaccaggagc tcgatcaggc catcggcaat gaggatgcgc
     1861 gatccgggct tgatcacctg gagaagctgc ggcaggttga tgccgatcac gccctcgcgg
     1921 gttgattcgg gcatcggcac gatgcggaca tcgctgccgc gggcgaaggg aacgggtccg
     1981 ctgccatcag gcagaggacc ggtgcggatc ttcaggccgc ggaggtcgaa caggatcggg
     2041 accggcttgc cgacttcatc cgcagcggca cggacgtcct cgatcaggcg gacgcgagtc
     2101 tcctcgctgc catgggcggc gttgatccgc accatgtcgg cgccggcgga gatcagatcg
     2161 accagcttat ccttgcctgc cacggcaggt ccgagagtcg ccacaatctt gacgcgacgc
     2221 tcaagtgaca ctggcattct ccccacgcgg ttccctcgct cttgcggcaa tgctacccga
     2281 accaattcag aacgtgactc cactaatcaa cataatgttc gcatacggcg tgcattcgcc
     2341 actgcgcacg atcgcgacgg cgccttcgca catttgcttg aactcctcgt ggggaacggt
     2401 gcggagttcg acgccgggga agagggctcg cacctcgttt ggaagcgcct ggttccggtc
     2461 gagcaattcg tccgccagga caacgccctc gacctgaagc tcgtgggcga tcgcccgggc
     2521 cagatcgagc acgcccggca gtccgggaac cacggcgaga tcgatccgct cgacgcccgg
     2581 cgggatcggg aggccggcgt cggcaatgac caatcgctgc gtatgtccca tccgggcgat
     2641 gacgtcggaa agccgatggt tcaggatgcc gttcttcttc acggaaaacc tcgcaatacg
     2701 gacactcgat taaggaagcg tagcgcgtca aacccttgcc ggggcgtgga gcggcgaaga
     2761 taggactgat gtccacgggc acaacccttc tggggacttg cgcagtgaca taagatcgaa
     2821 tcagcgaggt ttgattgcaa atgccgccgc gctcgtggcg tggcggcgtt caaaaggtgt
     2881 tcgccccgac gtacctggag gaatgacagg ctcccgccat gactccacca cgcgccgccc
     2941 gatcgccatt tacgctcctg accccaatcg tgatcggggt ggtcgtcgcg ggcgccgcag
     3001 ttatcttcct ggtcttcatg gtgattccgt cgcgcgggca ggccgaccct gatgcgcagc
     3061 ggccggacgc gccggcaatc aacgcgcctg gggtgtcgga gttgccggaa tcgaaaccgc
     3121 tcggccatcc gatcaatcgc atcaacgagc cgctgttcct tgaccccacc agtacgccgg
     3181 aagagttggc tatggtcgag ccgacgaagc gtccgacgaa gacgccgacc gaagaggttg
     3241 tggtcgagtt ctcctcgacg ccgagggtca tccggacacc cacgcccacc tacgaggcgc
     3301 cggtcattgt cgagcagccg acggaggcct gggtaccgac acaggcgccg ccgccagtcc
     3361 agccaccgcc acagccagcg accagcacgc cgcagccagc gccgaccgcg acgtcggtgc
     3421 cgccgacgat cgcgccccag ccgacgaaga ccgccgcacc gccgcctccg ccgccggcga
     3481 cagcaacgcc gatcccgctt tcgccgaccg cggtaccgcc gacgcctacc caaccgccaa
     3541 tcgagccgac cgcgactctg gtgaatgcga ccccgacggc cacggtgccg gtcatcgaag
     3601 ggccgccggt cactgaaacg ccgactgatg tgaccgccac gcccgattcg acgccggata
     3661 agaccgagac cccgacggcg acgagcactc caaaaccgaa gaagaagcgc acgccgactg
     3721 ccacgcccga cgtgaccgcc agttcggaag cgacgccggc taactgacca gcctgcgagc
     3781 cggcaagggc tgtgcgatca tgcccgcatg aatccgcaac cccgctccag gagaatgtga
     3841 tggcgtcgat cgaccgggtt ccgctcggcc aaaccggcct tgtggtgccg ccaattgccg
     3901 tgggatgcgc agagctcggg aacatgcccg aggccttcga ctatggcgtc tcggaagagg
     3961 atgcgctggc gacgcttcgt gccgcgctcg acagcccgct ctactacatc gacacggcgg
     4021 cctggtacgg caacggcgag agcgagcgcc gggtcgggaa ggtcctgcgc gagcgcggcg
     4081 gtctgccgga gggcgcgatc ctcgagacca aggccggtcg caaccccgat ggcgacgact
     4141 atcgaccgga gacggtcaag gcccggttcg agcggagtct cgaattgctc ggtgtcgacc
     4201 gggttcacat ttgcttcatc catgatgcgg aatggaccag gttcgaggac gtgatggcgc
     4261 cgggcggcgt gatggagatc ctgcaggatt acaagcggca gggcgtaatc ggcgcgctcg
     4321 gcatcgcggc tggaccggtc gagctctcga tgcagtatgt ccagaccggg atgctggatc
     4381 tgctcctgac gcacaaccgg tacacgttgc tcaaccgcgc ggcggacgcg gtccagaagg
     4441 aggcgaagcg gggaggcatg ggcgttgtca atgcggcgcc gtatggctcc ggcatgctgg
     4501 ccaagggtcc ggatgcgtac gcgcgatatg cgtatcagtc cgcctcggac gagatgatcg
     4561 aggtcacgcg cacgatccag cagatctgcg ataggcacaa cattccgctg gcggctgccg
     4621 cgctgcagtt ctcgatgctc gacccgaacg tagacgtcac gatcgtgggc atgagcaagc
     4681 ctgagcggat ccaacagacc atcgatctgg caacggtcga gattccgcag gcggtgtggg
     4741 acgagttggg cacgttgccg acatttgacg aggacccgga gctgaatcgc tggagggcat
     4801 ccgggaagca accggtattt gtggccgcga tgggtgataa gtagtccagt agttgaacag
     4861 gagtaactgc gttggctgat ttcgatacgt tgattgccgg gggacacgtc attgacccgg
     4921 cgcgtgggtt gtctggagcg gcggatgtcg cgatcaagga cggcaagatc gccgcggttg
     4981 aggccggtat cgaccggtcg cgcgcggcga aggtgatcga cgccgccgga caatacgtca
     5041 cgccgggcct gatcgacctg cacacgcatg tctactgggg cgtgacgtat tggggtatcg
     5101 agccggatcc ggtcgcggcg cgcagcggtg tgacgacctg gctggatgtc ggtagcgccg
     5161 gcggctacac cttccccgga ttccggcggt ggatcgccga gccggccaaa tcccgcgtct
     5221 ttgccttgct caacatgtcg tcgatcggcc tggtcgcgcc gacctgggag cttgcgaata
     5281 tcgattattg cgacatcgat ctcggccaga tgatcgtcga ccggaaccgg gacatcatcc
     5341 tgggcatcaa ggcgcggatc gactccagca cgacgcgcgg caccggcatc gagccgctca
     5401 agcgcgctcg cgatcttgcc gacaaggtcg agctgccgct gatggtccac atcggcgcgg
     5461 ctccgccgac gctggccgag atcgtcgagt acttgcggcc gggcgatatc ctgacgcact
     5521 gcttcaccgg tcagccgaac aagatcgtcg acgatgccgg cgcggtgatc ccgtcgatca
     5581 aggatctgca tgatcgcggg ctggtgctcg atatcgggca cggcgccggc tcattcagct
     5641 tcgagactgc cgaggcgatg ctgcagcagg gcatcctgcc ggacgtgatc agcaccgaca
     5701 tccaccagct cgcgatccag ggaccgtgct tcgatatgcc ggtgacactc tcgaagttcc
     5761 tcaacctggg catgaccatc gagcaggtaa ttgcctgcgc gacgacgaac gccgccaagg
     5821 cgatccgcaa gactgacttg ggcacactct gcgtcggctc ggtcgccgac gtcgcgacgt
     5881 tccgcatcga cgagggcgac ttcaccttcc aggacgtggc tatgaactgc cggaagggca
     5941 gcaagctgct ggtcaacacc atgaccatga tcgacggtga cgtgctgccg gtggtcgacg
     6001 atctgccgct gcagccgtgg gccaagctgc cggcgcatca ggcgggcaag gtgataccgg
     6061 tgaggcaggt gtagctgagg cgcggagacg gcaaggcgcg gaggcgcgaa gttgtagcgt
     6121 cgcatggcag agcgacttct ggtttgggcc gggtggtttc gtttgccctc cggctttcgc
     6181 atttttgccg aattcttgtt gcgcttcgat tgccaactcc gcgagcacgc tgtaatatcc
     6241 gactactttg gtcggatatc gcggtgcgag gagcttgcgg atgtttgata cgggtcgcgc
     6301 ggaggtcggt gtcgatcgcg gacgggcggt cgggtgggcc gttctggtca cactggcggt
     6361 ggcagtgctc tcgatcctgg tctggcaagg cgtgaccgcg gacggcgctc ctgatccgac
     6421 cgtgcctggt ctccatcacc gcgcaatcgt gctcgatgcc ggcgtattgg tcttccgcga
     6481 ggggctggaa gcgatccttg tactggccgc gatcagctcc agcttcgtcg gcgcgcagtc
     6541 cacatttcga cgaccggtcg ccgtcggttc cggcgcggcc atcctggcga ctgtcgcgac
     6601 ctggttcatc gtgatcgcca ttattggggc actcaatgcg cctgcactcg atattcaggc
     6661 gggcactggt ctcctcgcgg tcgtagtcct gctcgttgtg atgaactggt ttttccaccg
     6721 catatattgg accggctgga ttgcccatca caatcgccgc aggcgtgatt tgttggcttc
     6781 gggtggatcg ggcgtgttgc ttggcctcgc gctgcttggc ttcaccgcgg tctatcgcga
     6841 ggggttcgag atcgtcctgt tcctgcagac gctgcggttg caggccggat cgggcgttgt
     6901 gctgcagggc gtggcgatcg ggctcggatt caccgcggcg gtc
//
LOCUS       DATAOZ010000094         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7142562, whole genome shotgun sequence.
ACCESSION   DATAOZ010000094 DATAOZ010000000
VERSION     DATAOZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7142562"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..471)
                     /locus_tag="VHR64_02690"
     CDS             complement(76..471)
                     /locus_tag="VHR64_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301760.1"
                     /translation="MTKDDDGRRPETGGILVGLVIMFVGLAMLIDRMGISSIHLSGRF
                     WPFVLIALGCARLLTSSTAADGRRPSTWSGVWFIYLGLWFFINEFHLIGFWYTSSWPL
                     LVVGAGIGMIWRAIENAGRRASQGVTEGR"
     gene            530..>1924
                     /locus_tag="VHR64_02695"
     CDS             530..>1924
                     /locus_tag="VHR64_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEX3301761.1"
                     /translation="MFPANALTPPVNGESLVKSPPTMSCSSNELRAIRASAVWRVWLG
                     TALLSLLAVALPANTVRAGELQDDLKARRTRAMERLGPDALAIFWSAPTRVYSNDVNY
                     EYRQDSNLLYLTGIDQEDTILVLMPGNETRQEILFVREADARREHWNGHSLTPEEAST
                     ASGIQAVMTGNQFEPFIAAIFSRRATAASPTEYSRFLQALTDGRAKLALLLDPVTNLS
                     EPPSPVAQFASRVRERFFGFNVQDSSPILAELRQIKTSYEQTVLRRSVEISSDAHKAG
                     MLAAREGRFEYEVEAAIEAVYLQNGAMSWGYPSIVGSGPNATILHYEKSSRRIEPGDL
                     LLVDAAANYQGITGDITRTYPVDGKFSREQRDIYDIVYAAQQAGEKAAKIGGQARDIQ
                     AACDEVLRAGLVRLGLVTDPTGMQFKIWATHGVTHWIGMDVHDVGVPRKPLDDGMAFT
                     IEPGIYIREAALNDL"
BASE COUNT          386 a          593 c          604 g          341 t
ORIGIN      
        1 gcagcccgat gacgatgatc aggaggccca tcaccagctg cggcgcgatc ggtcgggtcc
       61 gctctgccgg ctgcatcatc gaccttccgt cacgccttga gaagcgcgac ggcccgcatt
      121 ctcgatcgcg cgccagatca tgccgatgcc ggcaccgacg acgaggagcg gccatgatga
      181 ggtgtaccag aagccgatga ggtgaaactc gttgatgaag aaccacagac cgagatagat
      241 gaaccagacg ccactccacg tcgacgggcg acgaccgtcg gctgccgtcg acgaggtgag
      301 caatcgcgcg catccgaggg caatcagcac gaatggccag aatcggccgg acaagtgaat
      361 gctcgagatt cccatccgat cgatcagcat cgccaggccc acgaacatga tcaccagtcc
      421 gaccaggatt cccccggtct caggacggcg cccgtcgtcg tctttggtca tgcgtcctct
      481 cggtagtgcc gacgctccga gaatacggcg atccctgtcg cgtttcccgg tgtttccggc
      541 gaacgccctc acaccgccgg tgaacgggga atctctggta aaatcgccgc ccaccatgtc
      601 ttgttcatcg aacgaattgc gcgcgatccg cgcgtctgca gtgtggcgcg tctggcttgg
      661 aacggccctc ctgtcgctgc tcgcagtcgc actgccagcg aatacggtgc gtgcgggcga
      721 acttcaggat gacttgaaag cccggcggac acgggcgatg gaacggctcg gacctgatgc
      781 cctcgcaatc ttttggagcg cgccaacgcg cgtgtattcg aacgatgtca attatgagta
      841 ccggcaggac agcaacctcc tgtatctgac cggaatcgat caggaagaca cgattctggt
      901 gctgatgccg ggcaacgaga cgcgtcagga aattctgttc gttcgcgagg ccgatgccag
      961 gcgcgagcac tggaacggcc acagcctgac acccgaggaa gcgtccaccg ccagcggcat
     1021 tcaggcggtc atgacgggaa accagttcga accgttcatc gccgcgatct tttcaaggcg
     1081 cgctaccgct gcgtcaccga cggaatactc gcggttcttg caggcgctga ccgacggccg
     1141 cgcgaaactg gccctgctcc tcgatccggt gaccaacttg tcagagccgc caagtccagt
     1201 cgcgcagttc gcgtcgcgcg tgcgcgagcg gttctttggt ttcaacgtcc aggattcctc
     1261 gccgattctc gccgagctgc ggcagatcaa gaccagttac gagcagacgg tgctgcggcg
     1321 cagtgtcgag atttcaagcg atgcgcacaa ggccggcatg ctggcggcgc gcgagggcag
     1381 gttcgagtac gaagttgaag cggcgattga agcggtctac ctgcagaacg gcgccatgag
     1441 ttggggctat ccgtcgatcg ttgggagcgg cccgaacgcg accatcttgc actacgagaa
     1501 atccagtcgg cggattgagc cgggcgatct gttactcgtc gacgccgcgg ccaactatca
     1561 ggggatcacc ggcgacatca cgcgaacgta tccggttgat ggaaagttct cccgcgagca
     1621 gcgcgacatc tacgacatcg tctacgccgc gcagcaggcc ggtgagaaag ctgcgaagat
     1681 tggtggacag gcgcgtgata ttcaggcggc ctgcgacgag gtcctgcgtg ccgggctcgt
     1741 gcgcctcggt ctggtcaccg atcccaccgg gatgcagttc aagatctggg cgacccacgg
     1801 tgtgacccac tggattggga tggacgtgca cgacgtcggc gttccacgca aaccgctcga
     1861 tgacggcatg gcgttcacca tcgagccggg tatctatatt cgagaagcgg cgctgaacga
     1921 cctg
//
LOCUS       DATAOZ010000095         4888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5301971, whole genome shotgun sequence.
ACCESSION   DATAOZ010000095 DATAOZ010000000
VERSION     DATAOZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5301971"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="VHR64_02700"
     CDS             complement(<1..1585)
                     /locus_tag="VHR64_02700"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEX3301762.1"
                     /translation="MDENLGAVQLASIETEMRSSYLDYAMSVIVQRALPDARDGLKPV
                     QRRILYAMSELGMRSNSRYRKSAGVVGEVLKNFHPHSDTAVYDTLVRMAQDFNLRYPL
                     IDPQGNFGSVDGDGAAAMRYTECRLAPIADEILSDIDKNTVDFKPNYDASTKEPKVLP
                     ATLPNLLVNGAVGIAVGMATNIPPHNLSEISDAIRYLIDNPECTVEDLMEIVQGPDFP
                     TGGTILGREGIRAAYSTGRGRVVIRAKAFVEESNRGNRFQIVVTELPYQVNKAALLER
                     IAELVKDGKIDGISDLRDESDRSGMRMIIELKRDSQPMKVLNNLYKHTAMQQTFGINM
                     LALVEEGMVPRVLTLKRALQEYVSHRQIVITRRTEYELERARKRAHILEGLKKALDII
                     DQIIATIRKSRTTETARNNLISEYQFSEVQAQAILDMRLARLAALERKKIEDELKEVL
                     KEIKYLEGLLADPKKILGLISDDMTRLKSSYGDERMTHIQDGSGEITDEDLTPQHGVL
                     VTLTKRGYVKRINEDVYRTQ"
     gene            complement(1753..2937)
                     /locus_tag="VHR64_02705"
     CDS             complement(1753..2937)
                     /locus_tag="VHR64_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX3301763.1"
                     /translation="MAEGANGTNGHHANGANVHSKRNDGKVRVAIIGAGNCASSLVQG
                     VQYYKDAPDDQFVPGLMHVNLGGYHIRDIEFTAAFDIDADKVGKDLSEAIFSGPNNTI
                     KFSDVPNLGVKVERGMTHDGLGKYLSQIITKAPGPTANVAKILKDTETDVVISYLPVG
                     SEMATKWYVEQVLEAGCAFVNCIPVFIASQEYWANRFKERGVPIIGDDIKSQVGATIT
                     HRVLTRLFSDRGVRIDRTYQLNFGGNTDFLNMLERERLDSKKISKTNAVTSQLDYAVD
                     AKNVHVGPSDYVPWLEDRKWCHIRMEGTTFGDVPLNVELKLEVWDSPNSAGVVIDAVR
                     CAKLALNNGIGGQLLAPSSYFMKSPPVQYHDDDARDQVEDFIKKNARKKSAVKEQAKA
                     EA"
     gene            complement(3067..4077)
                     /locus_tag="VHR64_02710"
     CDS             complement(3067..4077)
                     /locus_tag="VHR64_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX3301764.1"
                     /translation="MAILDLIQHANEYPDEIVHRVPESGPGEFRLGSQLVVREAQQAV
                     FVRDGKALDVFGPGRYTISTNNIPLLTALIGLPFGGSSPFTAEAYFISMREFLDLKWG
                     TPQPLVYRDADFGMIRLRAHGTYAIRVDNPQMFVNQIVGTRGSYSVNDLDDYLRSIII
                     NQFNDLLGETHKSILDLQGITLEMATTAQNALTTAFNQLGLRLIGFQIAGITPPDEVL
                     KKIDERSGMAAVGDMGAYTQFQAAQAMRDAAQNQGAAGGAAGTGVGFGAGMAMGQMMG
                     QAVNQATSGQSQQGQPAQQQQSGQNTVTPAGSAFCAQCGKPLPPGAKFCPNCGTPVAP
                     AE"
     gene            complement(4196..>4888)
                     /locus_tag="VHR64_02715"
     CDS             complement(4196..>4888)
                     /locus_tag="VHR64_02715"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX3301765.1"
                     /translation="RLSRLVRANVNDMLDRAEDPELMLDQILRDMESNIQLARGQVAS
                     MIAQEKELQADLKEVQDLSNEWQTKAGRAVEAGKDDLAREALRRKKDNDESATLYQQQ
                     LTTQTQTIEKMKSQLTALESKYQSTLSHRDELIARQRRAKSTQKVADTVSTFSPMDPS
                     AELDRIERKIRGTEARAEATIEMHDDSLDSQFAELDYDTDVEAELEALKASVGKPPEP
                     LGTGDHMTGPSA"
BASE COUNT          803 a         1456 c         1560 g         1069 t
ORIGIN      
        1 tctgtgttcg gtacacgtcc tcgttgattc gcttgacgta gccgcgcttg gtcaatgtga
       61 ccagtacgcc gtgctgtggg gtgagatctt catccgtgat ctcgcctgag ccgtcctgaa
      121 tgtgggtcat ccgctcgtcg ccgtagctgc tcttaagtct ggtcatgtcg tccgagatca
      181 gaccgaggat cttcttcgga tcagcgagca ggccttcaag gtacttgatt tccttgagga
      241 cttccttgag ctcgtcctcg atcttcttgc gctcgagcgc ggcgaggcgc gccagccgca
      301 tatcgaggat ggcctgtgcc tgaacttcgc tgaactggta ctcgctgatc aggttgttcc
      361 gcgccgtctc ggtcgtgcgc gacttgcgga tggtggcgat gatctggtcg atgatgtcga
      421 gcgccttctt gagaccctcg agaatgtggg cgcgcttgcg ggcccgctcc agctcgtact
      481 cggtccgccg cgtgatcacg atctggcggt gcgagacata ttcctgcagc gcgcgcttga
      541 gtgtcagcac gcgcggcacc atgccctctt cgacgagggc cagcatgttg atgccgaagg
      601 tctgctgcat ggccgtgtgc ttgtacaggt tgttcagcac cttcatcggc tgcgagtcac
      661 gcttgagctc aatgatcatg cgcatgcctg agcgatccga ctcgtcgcgc aggtcggaga
      721 tgccgtcgat ctttccgtcc ttgaccagct cggcgatgcg ctcgaggagc gcggccttgt
      781 tgacctggta ggggagctcg gtcacgacga tctggaagcg attgccgcga ttcgactctt
      841 cgacgaacgc cttggcccgg atcacgacac ggccgcggcc ggtcgagtag gcggcgcgaa
      901 tgccttcccg cccgaggatc gtgccgccag tcggaaagtc cggcccctgc acgatttcca
      961 tcaggtcctc gaccgtacac tcggggttgt cgatcagata gcggatggcg tcggatatct
     1021 cgctcaagtt gtgcggcggg atattggttg ccatgccgac ggcgatgccg acggcgccgt
     1081 tgaccagcag gttcggcagc gtggccggga gcaccttcgg ctccttggtg ctggcgtcgt
     1141 agttgggctt gaaatcgacc gtgttcttgt cgatgtcgct gagaatctcg tcggcgatcg
     1201 gagccagacg gcactcggtg taccgcatgg ccgccgcgcc atcgccatcg accgatccga
     1261 agttgccctg cgggtcgatc agcgggtagc gcaggttgaa atcctgcgcc atgcgcacga
     1321 gcgtgtcgta gacggcggtg tcgctgtgcg ggtggaaatt cttgagcacc tcaccgacga
     1381 cgccggcgct cttgcgatac cgggagttgc tgcgcatgcc caactcagac atcgcgtaca
     1441 ggatgcgccg ctgaaccggc ttgaggccgt cgcgcgcgtc cggcagagca cgctgcacga
     1501 tcacgctcat cgcgtaatcg aggtagctgc tccgcatctc agtttcaata ctggcgagtt
     1561 gaactgcgcc gagattctcg tccaatgtgg gggattcctt gctcgctgaa acgcgaattc
     1621 ttacccgaat agtttaccag attatcggcc tctccggcca cccaaaaacc cgcgtacgga
     1681 cgcccaaaac accacgaaaa cggggacggc tgtgaggccg tccccgtcgt tttctgtaga
     1741 agtgtccttg gcttacgcct cggctttggc ctgctccttg accgcgcttt tcttgcgggc
     1801 gttcttcttg atgaagtcct cgacctgatc acgcgcatca tcgtcatgat actggactgg
     1861 cggcgacttc atgaagtatg agctcggtgc cagcagttga ccgccgattc cgttattgag
     1921 cgccagcttc gcgcagcgaa ccgcgtcgat gacgacgccg gccgagttcg gggagtccca
     1981 gacctcgagc ttgagctcga cattgagggg aacgtcgccg aaggtcgtgc cttccatgcg
     2041 gatgtggcac cacttgcgat cctcgagcca cggcacgtag tcggacgggc cgacgtggac
     2101 gttcttggca tcgaccgcgt agtcgagctg cgaggtaacg gcattcgtct tcgagatctt
     2161 cttggagtcg agccgctcgc gctcgagcat gttcaggaag tcggtgttgc cgccgaagtt
     2221 gagctggtag gtgcggtcga tgcgcacgcc gcgatcggag aagaggcggg tgaggacgcg
     2281 gtgggtgatt gtcgcgccga cctggctctt gatgtcgtct ccgatgatcg gaacgccgcg
     2341 ctccttgaag cgattcgccc agtattcctg gctggcgatg aagaccggga tgcaattgac
     2401 gaaggcacag ccagcctcga gcacctgctc gacataccac ttggtggcca tctcggagcc
     2461 aaccggcagg taggagatga cgacgtcggt ctcggtgtcc ttgaggatct tggcaacgtt
     2521 tgcagtcggg ccgggcgcct tggtgatgat ctgcgagagg tacttgccga ggccgtcgtg
     2581 ggtcatgccg cgctcgacct tgacgccgag attcggcacg tcggagaact tgatggtgtt
     2641 gttcggaccg ctgaagatcg cctcgctgag gtccttgccc accttgtcgg catcaatatc
     2701 gaaggcagcc gtgaactcga tgtcacggat gtggtagccg ccgaggttca cgtgcatcaa
     2761 gccgggaacg aactgatcgt ccggcgcatc cttgtagtac tgtacgccct ggacaagtga
     2821 cgaggcacag ttgcctgcac cgatgatcgc cacgcggacc ttgccatcat tccgcttcga
     2881 atggacattc gcgccgttcg cgtgatgacc gttcgtaccg ttcgcgcctt ctgccacgct
     2941 tgctgctcct gagaagatcg tgacgatccg catgcaaggg atcgccggac tgtgaaaagc
     3001 cgccggaagt ctaggcgcgc ccgtgctggg ccgtcaacgc tttctttcgg ctagtgtcaa
     3061 aagccgctat tcagcgggtg cgaccggcgt gccgcagttt gggcagaatt tggcgcccgg
     3121 cggcaatggt ttgccgcact gcgcgcagaa tgccgatcca gcgggcgtga ccgtgttctg
     3181 tccggactgt tgctgctgag ccggttggcc ctgctgcgac tggccagacg tggcctgatt
     3241 aaccgcctgg cccatcatct ggcccatcgc cattccggcg ccgaacccca cgccggtgcc
     3301 tgccgcaccg ccggccgctc cctggttctg ggcggcatcc cgcattgcct gcgcggcctg
     3361 gaattgggtg taggcgccca tgtcgccaac ggcagccatt ccgctgcgct cgtcgatctt
     3421 cttgagcacc tcatctggag gcgtgattcc ggcaatctgg aatccgatca gtcgcagacc
     3481 gagctggttg aacgcggtcg tcagagcgtt ctgggcggtc gtggccattt cgagcgtgat
     3541 gccttgcagg tcgagaatcg acttatgcgt ctcgccgagc aggtcgttga actggttgat
     3601 gatgatgctg cgcaggtagt cgtcgaggtc gttgactgaa taggagccac gggtaccgac
     3661 gatctggttg acgaacatct gcgggttgtc gacccggatc gcataagtgc cgtgcgcgcg
     3721 cagccggatc atgccgaagt cggcatcgcg gtaaaccagc ggctgcggcg tgccccactt
     3781 gagatcgagg aattcccgca tcgagataaa gtaggcctcg gccgtgaacg gggagctgcc
     3841 gccgaaaggc agtccgatca gcgcggtcag gagcgggatg ttgttcgtcg agatggtgta
     3901 tcggccggga ccgaacacgt ccagcgcctt gccgtcccgc acgaagaccg cttgctgggc
     3961 ctcgcgaacg acaagctgcg atccgagacg gaactctccc gggccgcttt ccgggacgcg
     4021 gtggacgatc tcgtcggggt actcatttgc atgctgaatc agatcaagaa tcgccatcgc
     4081 agcttgcctt tctcatcagg ttttggattt caggttttag gcgggtggtt tggcgatccc
     4141 aggtctttgg cgggagtgcg caaaacctgt aacccaaaac ccaaaacctc tttcgctaag
     4201 cggaggggcc ggtcatgtgg tcgccggttc ccagcggttc cggcggcttg ccgacactgg
     4261 ccttgagcgc ttcgagctcc gcttcgacgt cagtatcgta atcgagctcc gcgaattgcg
     4321 agtcgagcga gtcgtcgtgc atctcgatgg tcgcctcggc gcgtgcttcg gtgccgcgaa
     4381 tcttgcgctc gatgcggtcc agctcggcgg agggatccat cggggagaag gttgaaaccg
     4441 tgtccgcgac cttctgcgtc gacttggctc gccgctggcg cgcgatcaat tcgtcgcggt
     4501 ggctgagcgt cgactggtac ttcgactcaa gagcggtgag ctgcgacttc atcttctcga
     4561 tcgtctgcgt ctgcgtcgtg agctgttgct gatacagcgt ggcgctctcg tcgttgtcct
     4621 tcttgcggcg cagcgcttcg cgggcgaggt cgtccttgcc ggcttcgacc gcccgaccag
     4681 ccttcgtctg ccactcattg gacagatcct ggacctcctt gaggtcggcc tggagctcct
     4741 tctcctgcgc gatcatcgac gcgacctggc cgcgagcgag ctggatattg ctctccatat
     4801 cgcggaggat ctgatcgagc atcagctcgg gatcttccgc gcggtcgagc atgtcgttga
     4861 cattggcccg gaccaggcgt gagagtcg
//
LOCUS       DATAOZ010000096         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3196678, whole genome shotgun sequence.
ACCESSION   DATAOZ010000096 DATAOZ010000000
VERSION     DATAOZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3196678"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VHR64_02720"
     CDS             <1..624
                     /locus_tag="VHR64_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3301766.1"
                     /translation="VATLVCLCSIPVMVAIASAVLFGERPSRVVSLALIGAVGGTALL
                     TVGQAGSAGKGGLAVGIGASLLSAVGATIYTLGSRAIVQRYHPITMLALGFPITLVLF
                     SPVMRGGHVSAGIPASAWLLLIYLGVGTQGIAYLFYQWGLKTESATVASIVTLIEPVL
                     AAFLAWVLFDERLGLLGIVGALLLIAGLMLLSFSPRPATINADGRAD"
     gene            641..838
                     /locus_tag="VHR64_02725"
     CDS             641..838
                     /locus_tag="VHR64_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301767.1"
                     /translation="MDEDPLKVEEDPQPVDMLESGPRETARIPSETVGTGSYVALSCT
                     VMTILATLLLIAGLLIYRWLS"
     gene            complement(835..1752)
                     /locus_tag="VHR64_02730"
     CDS             complement(835..1752)
                     /locus_tag="VHR64_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX3301768.1"
                     /translation="MTSIGYVTVDVFTSSRFGGNPLAVIPDARGLSDDAMAAIAREFN
                     YSETTFVLPPADPANTARVRIFTPTSELPFAGHPNVGTGYVLGRLGSLFDRPITDALR
                     FEENAGLVVVELIRDGAEVAGATITAPQSLSLGPELDAAEVAECIGLPPSAILTTHHQ
                     PQYASVGLPFLCVEIGDLDSLGRAVPNTMQFRAAQDELPDRALEFNVFAYTETSTNPL
                     SVRARMFAPLDGVPEDPATGSAAGALGAFLTHLRPEADLELTFPILQGVEMGRPSRID
                     LAVRKGRGVIERVGISGRCVDVMRGTFEL"
     gene            complement(1782..2159)
                     /locus_tag="VHR64_02735"
     CDS             complement(1782..2159)
                     /locus_tag="VHR64_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3301769.1"
                     /translation="MVPTAFCPTFHRAVELIGKRWSGAIIRAMRSGRVRFSDISDAVP
                     GLHDRLLSERLKELEHEGIVERVVIPETPVRIEYRLTCKGQDLDGVMDAVSLWAERWV
                     TVTPEEVSVDAECAEAEQAALTR"
BASE COUNT          408 a          724 c          776 g          469 t
ORIGIN      
        1 gttgccacgc tggtctgcct ctgttcgatt ccggtcatgg tcgcgattgc gtcggctgtc
       61 ttgttcggcg agcgaccgtc gcgcgtggtt tctctggccc tgattggggc agtcggagga
      121 acggcgctac tgacggttgg ccaagctgga tcggccggca agggcggact cgccgtgggg
      181 atcggagcct cgctcctctc agccgtcggt gccacgatct ataccctcgg cagccgcgcg
      241 atcgtgcagc ggtatcaccc gatcacgatg ctcgcgctcg gtttcccgat cacgctggtg
      301 ctgttttcgc cggtcatgcg gggcggccac gtctccgctg ggatcccggc gtcggcctgg
      361 ttgttgctga tttacctggg cgtcggcacg caaggcatcg catacctctt ctaccagtgg
      421 ggcttgaaga ccgagtccgc caccgtggcc agcatcgtga ccctgatcga gccggttctg
      481 gccgcgtttc tggcctgggt gctattcgat gagcggcttg ggttactcgg catcgtaggc
      541 gcattactgc tgatcgcagg gctcatgctc ctgagcttca gtccgcgacc ggctactatc
      601 aacgcggatg gtcgggccga ttgatgcggc agggagtgga atggacgagg atccgctgaa
      661 ggtcgaagaa gatccgcagc cggtggacat gcttgagtcg ggtccgcgcg agacggcgag
      721 gatcccgtcc gagacggtcg gcaccggatc gtacgtcgcg ctctcgtgca cggtcatgac
      781 gatcctggct acgctcctcc tgattgcggg actgctaatc taccgctggt tgagctagag
      841 ctcgaacgtc cctcgcatca cgtcgacaca tcgaccgctg attcccaccc gctcgataac
      901 ccctcggccc ttgcggaccg cgaggtcgat ccggctcggc cgacccatct cgacgccctg
      961 caggatcggg aaggtgagtt cgaggtcggc ctccggtcgc aggtgggtca gaaatgcgcc
     1021 gagggctccc gcggcactgc cggtggccgg atcctccggt acgccatcaa gcggcgcgaa
     1081 catgcgggcg cggaccgaga ggggattggt cgacgtctcg gtgtatgcga agacgttgaa
     1141 ctccagggct cgatccggca gctcgtcctg cgcggcgcga aactgcatcg tgttgggcac
     1201 tgcccgcccc agcgaatcca ggtcaccgat ctcgacacag agaaagggca ggccaaccga
     1261 cgcgtattgc ggctggtgat gtgtcgtcaa gatggctgac ggtggcaggc cgatgcactc
     1321 ggcaacctcg gccgcgtcca actctgggcc caggctgagc gattgcggcg ccgtgatcgt
     1381 ggcgccggcg acctctgccc cgtcgcgaat cagctcaacg acgacgagcc cggcattttc
     1441 ttcgaaccgg agcgcgtctg ttatcggccg gtcgaacagt gacccgagcc gccccagcac
     1501 gtatccggtg ccgacattgg ggtgaccagc gaatggaagc tcggaggtcg gtgtgaaaat
     1561 gcggacccgc gcggtgtttg ccgggtcggc cggggggagc acgaaggtgg tttcggagta
     1621 attgaactcg cgcgcgatgg cggccatcgc gtcatccgag agaccgcggg cgtccggaat
     1681 cactgccagg gggttgccgc cgaaacggct ggacgtgaag acatcgaccg tcacataccc
     1741 gattgaggtc atgcaggcgc gactcccggt atcgggcagc gttagcgagt gagggcggct
     1801 tgttccgcct cagcgcattc cgcgtccacc gaaacctctt ccggggttac cgtcacccat
     1861 cgctcggccc agaggctgac ggcgtccatc accccgtcga gatcctggcc cttgcaggtc
     1921 aggcggtact cgatgcgtac cggcgtctcc gggatgacga cccgctcgac gataccctcg
     1981 tgctcgagct ccttcaggcg ctcggagagc aagcgatcgt gcaggccggg cacggcgtcg
     2041 ctgatatcgg agaagcggac gcgcccgcta cgcatggcgc ggatgatcgc gccgctccag
     2101 cgcttgccga tcagctccac ggccctatga aacgtcgggc aaaatgcagt tgggaccact
     2161 ttgttttcca tgacgcaatt ttatatgatg atcgcgctct ttgcaacatc ttgttagcca
     2221 cttacttgac acaagcaact cactcatgta tagtaagtgc aatgaggttc gtaagcacgg
     2281 accggagata ttcctttgag agggagtgaa gtctagttat ggcaacgatt tggaagattg
     2341 atgaagcgca ctcttcggtt gagtttgtcg tcaagca
//
LOCUS       DATAOZ010000097        12474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2408123, whole genome shotgun sequence.
ACCESSION   DATAOZ010000097 DATAOZ010000000
VERSION     DATAOZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12474
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2408123"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VHR64_02740"
     CDS             <1..483
                     /locus_tag="VHR64_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3301770.1"
                     /translation="DVMTVKPIRAERLLATLERGWDALVGSYDGLTPEVITKPGVVGE
                     WSIKNLIAHVSIWEMEAIKHLPVIAAGNRPPRYSVRYGGIDAFNDQMMANMLGLSLEE
                     TLHQRDDTHQRLIAYLQSLPPELLATNERFRRRLRLDTYGHYAIHSADIQDWRASRKE
                     "
     gene            complement(541..2445)
                     /locus_tag="VHR64_02745"
     CDS             complement(541..2445)
                     /locus_tag="VHR64_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3301771.1"
                     /translation="MAQAPNAGPATPTPLGDVVPPELNTETNWAYENYDLHATRDVKG
                     SSIDSNSIAQLGDAWMFPVQSSAAFGALTANPTIVGTTVYIQDASANVYAINMETGEQ
                     LWANMYNDVVPSGGPNGITAAYGLLFTSLGGAGDVLALKADSGEEVWRTNILGPKNEG
                     ITTPPLVYDNMVIISTIPGSSDGFYIGGQRGVIHSLDAGTGAVLWYFDTTTDNLWGNP
                     TANSGGGFWHPPSVDDDGKLYVGIANPAPYPGEEGFPWASSRPGDNLYTDSILKMDRK
                     TATIDWYVQVKPHDVFDLDNQLTPIIADVDGKKLVFTSGKHGVVYAFDRDAGEQVWRT
                     PIGTHKNDERVDDFQEDEVVEVWPGTLGGVETQFAYSDANKLIIAPVVELSTTYIGTG
                     FDPNTPLDFTGGKGLLVALNAADGSIAWQVDLQTPPYAGATICNDVVFTAGLDGVLLG
                     FNVADGSKVFKYQATAGVNAMLAATGDYLIVPAGGPLVQTSEAWNPLPQQKAQVIALK
                     VGGTVQQQPSGTPQASPQAEEATPQPEQATPQGQENAGAVSVDMHEFAFTPNQITIAA
                     NTDVQVTATNSGALPHNFTVPDLNLKTEDVAPGSSATLTINAPAGSYKFDCSIPGHAD
                     AGMVGTLIVQ"
     gene            complement(2753..3328)
                     /locus_tag="VHR64_02750"
     CDS             complement(2753..3328)
                     /locus_tag="VHR64_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301772.1"
                     /translation="MIPTGTALAQEATPAPATCDVEPRPITFLADLLAAPKPEVTPTP
                     VAGVPDGTDVTDPVVRAEVEKVVQTLITCVNQGEILRAFSLYDDEFVRRLIDPDGIMG
                     SEIAIEVGKSLAKTVPAGPEDVTTLDEIVLVRALPDGTIAVVFRTHGGPDRDQDDSQI
                     DLFVLRKVNDRWLIIDGLTDLDPESLPTPTA"
     gene            3478..4122
                     /locus_tag="VHR64_02755"
     CDS             3478..4122
                     /locus_tag="VHR64_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301773.1"
                     /translation="MPASDFGNPLTVSVDLRLDLALRSSVEAWEAAIADRGPAATAKW
                     IMQRSAGADLEREMVIEAIENLIAAEDPADRALARAEVAEMVQGDDAELADALWFAVR
                     DFAIEEADGDLLTEATAHISEIAFDLDEPTSAAEAWIDFLNWRREPDSASDPEAVLTA
                     FDEIIRAAEMDGEHTTAAKYGYMQVQFQKLVEADDPRATVGNWLADDQLLGSWS"
     gene            4251..4868
                     /locus_tag="VHR64_02760"
     CDS             4251..4868
                     /locus_tag="VHR64_02760"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX3301774.1"
                     /translation="MAFQLPPLPYPNDALEPNIDAKTMEIHHDKHHAAYVSNLNAAIE
                     GNADLGSMSAEDLIKNLNKVPEDKRTAVRNNGGGHVNHTMFWEIMGPNGGGQPSGALA
                     AAIDRDLGGFEAMKEAVNKAGAGRFGSGWAWVVLQDGRLSVTSTPNQDNPMMDGSGTP
                     ILGVDVWEHAYYLKYQNRRPDYLGAWWNTINWDAVGKRYTDAGGK"
     gene            complement(4919..5440)
                     /locus_tag="VHR64_02765"
     CDS             complement(4919..5440)
                     /locus_tag="VHR64_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301775.1"
                     /translation="MSEDEILDQIDEGQTGSPAARITIPMATRKDFYTGIARRLMTLL
                     PPELRDFRHRGEFNLMKIWFNHYRIHYEVVLDQQINKIELGLHFEDGPASSLAFLALL
                     DRRILEIKDHLGPGIELERWTQSWVRLYELRPLVAIDDGVCDDCAQRLAAMIAYLQPI
                     IVESGIRLERPGA"
     gene            5665..6927
                     /locus_tag="VHR64_02770"
     CDS             5665..6927
                     /locus_tag="VHR64_02770"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX3301776.1"
                     /translation="MESTPPTGIPAEESPPVPEQAVAVEEPVIEAAPAPPQVLSGSPV
                     TIGVPREVVPGERRVALVPDIVKRLVAQGQRVVVETNAGEAAHLLDDAFTAAGATIVP
                     DHASVFQQADLIVKVQKPTPDEIGLLRRGQTLIAFLQPMVNTDLVQVLRDQGVTTFSM
                     DAIPRTSRAQYMDALSSMATVAGYKAVLLAADHMAKFMPLLTTAAGNIPPAKVLVIGA
                     GVAGLQAIATARRLGAVVEAYDTRPVVKEQVQSLGARFIEIDTGSTDTQTAAGYAREA
                     TAQELAAQQAELARRSARSDAIITTAAVPGRPAPRLISAETVEQMAPGSVIVDLAAET
                     GGNCELTQPGKVIDHNGVTIIGTLNLPSTLPIHASQMYAKNIQNLLALMVNKDGALIL
                     NMDDDIVAGTVITHNGQVLHEGTRARMG"
     gene            6938..7246
                     /locus_tag="VHR64_02775"
     CDS             6938..7246
                     /locus_tag="VHR64_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX3301777.1"
                     /translation="MDNSVLLGIYVFLLAIFLGIELIGRVPATLHTPLMSGTNAIHGI
                     VLLGGMVVLGEADTWWLQLIGFLAVVLGAANVFGAFVVTDRMLEMFKGRKPTPGADKK
                     "
     gene            7243..8649
                     /locus_tag="VHR64_02780"
     CDS             7243..8649
                     /locus_tag="VHR64_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX3301778.1"
                     /translation="MIDDGRQAIIDIAYLIAAVFFIFGLKFLSAPKQARRGNQLAAVG
                     MGIAILATFLSRGWESSNYILIIVGILIGGVAAGWAARVVKMTAMPQMTAIYNGMGGA
                     TAALVSLGEFSHKTNIGAGEKVAIVIGCIIGSISFTGSMVAFAKLQELLPGKPIRIAN
                     QQMYNLVAAGALVVLTILTVSLDHNTTTKGMLWVIFAIALALGLSVVLPIGGADMPVV
                     IAILNSLTGLAAAVAGLALDNQAMVIAGALVGASGSYLTVLMARAMNRSVFNVVFGSW
                     GAPEVAGPAAGSGEQLPIRETSAEDVAVQLAYANKVIIVPGYGLAVAQAQHTVRELAD
                     ELQERGVDVKYAIHPVAGRMPGHMNVLLAEANVPYEDLYEMEQINDDFPSTDVALVIG
                     ANDVTNPAARDDKTSPIYGMPILNVDEAQNIVVLKRSMKSGYAGIENMLFHDPKTSML
                     FGDAKTSLSELVTAVKEA"
     gene            8782..9639
                     /locus_tag="VHR64_02785"
     CDS             8782..9639
                     /locus_tag="VHR64_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3301779.1"
                     /translation="MAEQRGSARYEASYQTVLDAVALVPSAQRTTPGVCGDWSLKDVL
                     GHLAFWDGDLAVELEAKKNGVTLPTSSEREWQAINSEQAAIRAEWDWDDVLREVRENH
                     DRLVPVLNDPGENPNDEPIHEHWDEHHAQIDAWLTANGHAKDDLAPERSRDLYEELRR
                     SAVQTALGVPETLCDEPGVCGSWSLKELMGHLAFWDSRVADRLEARKAGRTWQRDSRS
                     YDVINAEAAAARSTCTWDEIQAELAETHARLLPLLSDPGEEGDYRIYMHWREHGAQIE
                     AWAAAHSHV"
     gene            9652..10122
                     /locus_tag="VHR64_02790"
     CDS             9652..10122
                     /locus_tag="VHR64_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301780.1"
                     /translation="MIQTVQPAPLTLDETIDRIEAAWRDWLLALEAVDPARRTEPGVC
                     GYWSVKDIMAHISLWENEIHEHIQRWQLGLPKSDLDVDQMNAAIVAENHVRPYALVRV
                     NMHRAHHDAMAAIRAIEGDLDDDIRDRIACETWDHYPEHTEQILTWLKRNQPDG"
     gene            10115..10540
                     /locus_tag="VHR64_02795"
     CDS             10115..10540
                     /locus_tag="VHR64_02795"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3301781.1"
                     /translation="MADRWLELLDSAWTRWLEALDQVDPARRNEKVLDDNWSIKDLVA
                     HTLFWDAEVLTDIQRWQHGRPPMSNDWQRMNDENHAAHRNRPYDLLRVEMHLVHQTVR
                     DAIAQLPADIPEELIERVAVDTWDHYDDHTQQIEGWLAK"
     gene            10563..11570
                     /locus_tag="VHR64_02800"
     CDS             10563..11570
                     /locus_tag="VHR64_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX3301782.1"
                     /translation="MKRTLAVIAIIIVVIAILRPSTIIDRSGRSQPTPTSTVVQTGGV
                     RGLVVLPDDGPNAILDEINQAKRSIDLYVYLLPSDQVLSALSAAHDRGVAIRVILERD
                     PFGGGNSNQDAYDRLAAAGIAVRWSSDQFQFSHIKTFVVDQRVAVVMTLNLSWSALTR
                     NREFAVVSTIPADVHEVSGLFEADWSAQPYSPEGDFVASPENSRAVFRGLIDNASISI
                     DIYAEVVRDTNIRQRLANAAARGVTVRILVPANPTADALQIYRDLERDGVQVRLLADA
                     YSHAKAIIIDGTSAFVGSQNLTQTSLDKNRELGMILTDQPNLDRLAHVFESDWSISPA
                     A"
     gene            12007..>12474
                     /locus_tag="VHR64_02805"
     CDS             12007..>12474
                     /locus_tag="VHR64_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX3301783.1"
                     /translation="MRNILALCAALAVLILGLGLATGARGQNVSYASPTPGETYCATP
                     VASMVGTPAIVVTAPTTAASPGGVAPGTAVGLVPCGTPIGTPADGGSQAQTAPTISMV
                     DIAYNPNQLTIPANTDVTVTLKNTGQLPHTFDIDALNIHSDQVQPGQSATVKIN"
BASE COUNT         2383 a         4042 c         3859 g         2190 t
ORIGIN      
        1 gatgtcatga cagtcaaacc aatccgggcc gaacggctcc tggccacgct cgaacggggg
       61 tgggacgcgt tggtcggctc gtatgacggt cttactccgg aagtgatcac gaagccgggc
      121 gttgtcggcg aatggtcgat caagaacctg atcgcccacg tctcgatctg ggagatggaa
      181 gccatcaagc acctgccggt cattgccgcc ggaaatcgcc cgccgcgcta ctcggtcaga
      241 tacggcggaa tcgacgcatt caacgaccag atgatggcca acatgctggg tctgtccctg
      301 gaagagacgc tccaccagcg cgacgacacg catcagcggt tgatcgccta cctgcaaagc
      361 cttccgccgg agctcctcgc caccaacgag cgcttccgga gacgacttcg cctcgataca
      421 tatggccact atgcgatcca tagcgccgac atccaggatt ggcgggcgag tcgcaaggaa
      481 tagcaaaccg gcgcggaatt gctcccgcgc cggtttgcat gttcagctgg accgatcggt
      541 ctattgaacg atcaaggtac cgaccatgcc cgcgtccgcg tgacccggga tcgagcaatc
      601 gaacttgtag ctgccggccg gcgcgttgat cgtcagcgtt gcgctactgc ccggcgccac
      661 atcctcagtc ttgaggttga gatccgggac cgtgaagttg tgcgggagcg cgcccgagtt
      721 ggtggccgtt acctgcacgt ccgtgttcgc cgcaatcgtg atctgattgg gagtgaacgc
      781 gaactcgtgc atatcgaccg agaccgcccc ggcgttctcc tgtccttgcg gtgtcgcctg
      841 ctccggctgc ggtgtcgcct cctctgcctg cgggctcgcc tgcggtgtgc cggatggctg
      901 ctgctggacc gtaccgccga ctttgagcgc gataacctgc gccttctgct gcggcagcgg
      961 gttccaggct tccgaggtct gcacgagcgg gccaccggcc gggacgatga ggtaatcgcc
     1021 cgtcgccgcg agcatcgcgt tgacgcccgc cgtggcctgg tacttgaaga ccttgctgcc
     1081 atccgcgacg ttgaaaccga ggaggacgcc atccagcccg gccgtgaaga cgacgtcatt
     1141 gcatatcgtc gcgccagcgt acggcggtgt ctgcaagtcg acctgccagg cgatcgaccc
     1201 atcggccgcg tttagcgcaa ccagcaagcc cttgccgccg gtgaagtcaa gcggcgtatt
     1261 cggatcgaac ccggtgccga tgtaggtcgt cgacagttcg actaccggcg cgatgatcag
     1321 cttgttcgcg tccgagtatg cgaactgcgt ctcgacgccg ccgagggtgc caggccaaac
     1381 ctcgacgact tcatcctcct ggaagtcgtc gacgcgctcg tcgttcttgt gggtgccgat
     1441 cggcgtccgc cagacctgct cgcctgcgtc ccgatcgaat gcatagacga cgccgtgctt
     1501 gccggaagtg aagacgagct tcttgccgtc gacgtcggcg atgatcggcg tgagctggtt
     1561 gtcgaggtcg aagacatcgt gcggcttgac ctgaacatac cagtcgatag tcgctgtctt
     1621 gcgatccatc ttgaggatcg agtcggtgta gaggttgtcg cccgggcggc tgcttgccca
     1681 ggggaagcct tcctcgcccg gatagggcgc cgggttggcg atgccgacat agagcttgcc
     1741 gtcatcgtcg acagatggcg gatgccagaa gccaccgcca gagttggccg tcgggttgcc
     1801 ccacaggttg tccgtcgtag tatcgaaata ccagagaacg gcgcccgtcc cggcatcgag
     1861 cgaatggatc acgccacgct ggcccccgat gtaaaagcca tcggacgagc ccgggatcgt
     1921 gctgatgatc accatgttgt cgtacaccag cggcggtgtc gtgatgcctt cgttcttcgg
     1981 tccgagaatg ttggtccgcc agacctcctc acccgaatcg gccttgagcg cgagcacatc
     2041 cccggcaccg cccagcgagg tgaacaggag cccgtaggcg gccgtgatgc cattggggcc
     2101 gccggacggc acaacatcgt tgtacatgtt tgcccagagc tgctcgcccg tctccatgtt
     2161 gatcgcgtag acgttcgcgc tggcatcctg gatgtaaacc gtggtaccga caatggtcgg
     2221 gttcgcggtc agggcgccaa atgcggcgga cgactgcaca gggaacatcc aggcgtctcc
     2281 aagctgggca atcgagttgc tatcgatcga gctccccttg acgtcacgtg tcgcatggag
     2341 gtcgtagttc tcgtatgccc aattggtttc cgtgttgagc tcgggcggaa cgacgtcgcc
     2401 gagcggcgtc ggagtagccg ggccggcgtt tggcgcttgc gccaaagcac gtgacccgct
     2461 ccggctagag tcaaggtaaa caccaccccc cgcaatggcg acgccagcag cgctggcagt
     2521 tgcgatcaat cgccgccggg acagcacctt atcgttcatt tcttctcctt cgcacacaag
     2581 gacaggctgg cgacgcccac gcgcgcttca agaattcaga ttcgccatcg cctgagcaat
     2641 tggggaattg gcccgccaac caggcaccac gtgcgtagca tacggagaag tcaggccgca
     2701 aaatcagccg aaacgaccaa tctcatgtgc ccgtgctaca aaggtcgccc ggtcaggcag
     2761 ttggtgttgg caatgactcg gggtcgagat ccgtcagccc atcgatgatc agccagcgat
     2821 cgttcacctt cctgagcacg aagagatcga tctggctgtc gtcctgatcc cggtccggtc
     2881 ccccgtgcgt ccggaagaca acagcgatgg tgccatcggg cagcgcgcgg acgaggacga
     2941 tttcatccag cgtggttacg tcttccgggc cggccggcac ggtcttggcg agcgacttgc
     3001 cgacttcgat cgcgatttca ctccccatga tcccgtcggg gtcgatcaac cggcgcacga
     3061 attcgtcatc gtagagcgag aaggcgcgca ggatttcgcc ctggttcacg caggttatca
     3121 gcgtctggac gaccttctca acctccgccc tgaccaccgg atcggtcacg tcagtcccat
     3181 caggaacgcc cgcgaccggc gtcggtgtga cctccggttt gggcgcggcg aggagatcag
     3241 cgaggaaggt gatcggacgc ggctcgacat cgcaggtcgc gggcgcgggc gtcgcttcct
     3301 gcgccagggc ggttcccgtc ggaatcacag gagttatcag aacagcaccg gttatcgcga
     3361 tcgcgaccgc gctcgtaggc catttgcttc gtcgggatcg gggcaaggtg gcaatctcca
     3421 gcctggctcg catacttcaa ccagtaagct cgcagtctcg catgaaatgg agaccaggtg
     3481 cccgcatcag acttcggcaa cccgctgacg gtatcggtcg atctccggct cgatttggct
     3541 ttgcgcagca gcgtcgaggc ttgggaagcg gccattgccg atcgcggacc ggcggcgacc
     3601 gccaagtgga tcatgcagcg cagcgccggc gccgacctcg agcgagagat ggtcatcgag
     3661 gcgatcgaaa acctgatcgc cgccgaggat ccggccgatc gcgcgctcgc ccgggccgag
     3721 gtcgccgaga tggttcaggg cgacgatgcc gaactggccg atgccctctg gtttgcggtg
     3781 cgcgacttcg ccatcgagga ggccgacggc gacctcctga ccgaggcgac ggcacatatc
     3841 tccgagatcg cattcgacct tgacgagccc acaagcgcgg ccgaggcctg gatcgacttc
     3901 ctgaactggc gccgcgaacc tgattccgct tccgatcccg aggcggtcct gaccgcgttc
     3961 gacgagatca tccgggccgc cgaaatggac ggcgagcaca ccaccgccgc caaatacggg
     4021 tacatgcagg ttcagttcca gaaactggtc gaggccgacg atccccgcgc tacggtcggc
     4081 aactggctcg ccgacgatca actgctcggg tcctggtcgt agcctgggtt cgggcgcaat
     4141 cgcttgtcgt tccggcactt ttggtaacct aggcgacgtt agcaatcgat cgccgggcag
     4201 cggttgcccg gcatctgaaa tgtctgtgat gacagaaagg aaagacctgg atggccttcc
     4261 agctaccgcc tctaccctac cccaacgacg ccctcgagcc gaatatcgac gcgaagacga
     4321 tggagatcca tcacgacaag caccacgctg cgtacgtgag caacctcaac gccgcgatcg
     4381 aaggcaacgc cgaccttgga agcatgagcg ccgaagacct gatcaagaac ctgaacaagg
     4441 tcccggagga caaacgcacc gcggttcgca ataacggcgg cggccatgtc aaccacacca
     4501 tgttctggga gatcatgggc ccgaatggcg gtggtcagcc gagcggagcg cttgcggcgg
     4561 cgatcgaccg ggacctgggc ggcttcgagg ccatgaagga ggccgtcaac aaggccggcg
     4621 ccggccggtt cggctcgggc tgggcgtggg tcgtcctcca ggacggccgg ctctcggtca
     4681 cgagcacccc gaaccaggac aacccgatga tggacggctc cggcacgccg attctgggcg
     4741 tcgatgtctg ggagcacgcc tactacctga agtaccagaa ccggcggccg gactacctgg
     4801 gcgcctggtg gaacacgatc aactgggacg cggtcggcaa acggtacacg gacgccggcg
     4861 gcaagtaatc ttcagattcg atcgacaaca cccggccagc gcaactggcc gggtgtcttc
     4921 atgccccggg gcgctcgagg cggatccctg attcaacgat gatcggttgc aggtacgcga
     4981 tcatggccgc aagccgctga gcgcagtcgt cgcaaacccc atcgtcgatc gcaaccaacg
     5041 gccgcagctc ataaagccgc acccacgatt gggtccaccg ctcgagctcg atcccggggc
     5101 cgagatggtc cttgatctcc aggattctcc gatcgagcag cgccagaaac gcgagcgagc
     5161 tggccggtcc atcctcaaag tggaggccga gctctatctt gttgatctgc tgatcgagca
     5221 cgacttcata atggatccgg tagtgattga accagatctt catcaggttg aattcgcccc
     5281 gatgccggaa atcgcgcagc tcgggcggca ggagtgtcat cagccggcgt gcgattccgg
     5341 tatagaaatc cttgcgcgtc gccatcggaa tcgtgatccg ggcggctggg gagccggtct
     5401 gcccctcgtc gatctggtcc agaatctcat cttcgctcat atcgggtaat catccccgaa
     5461 agccgtcgca acgaacagga gccgggcggc cccgatgccg gagtccgcca gtttcgttta
     5521 ccatttcact tcgaattagt gcctgacgga ggcgtcgggc cggcaacgac cctggctcgt
     5581 gccggatggt cgtttgcttc gtgtgcggtc agggcaactc ggaggcacga gagtgtcgcc
     5641 gatccctatg gagagtggag cgagatggag agcacgccgc cgaccgggat cccggcggag
     5701 gaatcaccgc ctgtgcccga acaggctgtc gcggtcgagg aaccggttat cgaggcagcg
     5761 cccgctcccc cacaggtgtt gagcggatcg cccgtcacga tcggtgtgcc acgcgaagtc
     5821 gtacccgggg agcgacgggt tgcgctcgtg cccgacatcg tcaagcgtct ggtcgcgcag
     5881 gggcagcgag tggtggtcga gaccaacgcc ggcgaagcgg cccatttgct cgacgatgca
     5941 tttacagccg ccggcgcgac gatcgtgccg gatcacgctt cggtcttcca gcaggccgat
     6001 ctgatcgtca aggtgcagaa gccgaccccg gatgagatcg gcctccttcg ccgcggtcaa
     6061 acactgatcg ccttcctgca gccgatggtc aacacggacc tcgtccaggt tctgcgcgat
     6121 cagggcgtga ccacgttcag catggacgcg attccacgaa cttcgcgcgc gcagtacatg
     6181 gacgccctct cctcgatggc caccgtcgcc ggatacaagg cagtcctgct cgcggccgat
     6241 cacatggcca agttcatgcc gctgttgacc actgccgccg gcaacatacc gccggccaag
     6301 gtgctggtga tcggcgccgg cgtcgccggg ctccaggcga tcgcgaccgc tcgtcgtctc
     6361 ggcgcggtgg tcgaggcgta cgacacccgc ccggtcgtca aggagcaggt ccagagcctc
     6421 ggcgcccgct tcatcgagat cgacaccgga tcgactgata cccaaaccgc cgccggctat
     6481 gcccgcgaag cgaccgccca ggagctggct gcgcagcagg ctgaactggc ccgccgttcc
     6541 gcccggtccg acgcgattat cacgacggcc gccgtgccgg gccgtccagc gccgcggctg
     6601 atctcggccg agacagtcga gcagatggcg cccggctcgg tcatcgtcga cctggctgcc
     6661 gagaccggcg gcaactgcga gctgacccaa cccggcaagg tcatcgacca caacggcgtc
     6721 acgatcatcg gcaccctgaa cctgccgagt acgctgccga tccatgcgag ccagatgtac
     6781 gccaagaaca tccagaactt gctcgcgctg atggtcaaca aggatggcgc gctgatcctg
     6841 aacatggatg acgacatcgt ggccggcaca gtcatcacgc acaacggaca agtgctccat
     6901 gagggcacac gggcccgaat gggatagggg ttgagggatg gataacagcg tactgctcgg
     6961 gatctatgta ttcctgctcg cgatcttcct cggcatcgag ctgatcgggc gagttccagc
     7021 cacgctccac acgccgctga tgtccggcac gaacgccatt cacggcatcg tgctgctggg
     7081 cggaatggtc gtgctgggcg aggccgacac ctggtggctg cagctgatcg gcttcctggc
     7141 cgtggtgctg ggcgccgcca acgtcttcgg ggcgttcgtc gtcaccgatc gcatgctcga
     7201 aatgttcaaa ggccgcaagc cgacgccggg ggctgacaag aaatgatcga cgatggtcga
     7261 caggcgatta tcgatatcgc ctatctgatt gcggctgtct tctttatctt tggactcaag
     7321 ttcctgagcg cgcccaagca ggcgcggcgg ggcaaccagc tggccgcggt cggcatgggc
     7381 atcgcgatcc tcgcgacctt cctcagccgc ggctgggaga gcagcaacta catcctgatc
     7441 atcgtcggta tcctgatcgg tggtgtagcc gctggttggg ccgctcgcgt cgtcaagatg
     7501 accgccatgc cgcagatgac cgccatctac aacggcatgg gtggcgcgac ggcggcgctt
     7561 gtctcgctcg gcgagttctc gcacaagacg aacatcggcg ccggcgagaa ggtcgcgatc
     7621 gtcatcggct gcatcatcgg atcgatctcc ttcaccggtt cgatggtcgc gttcgccaag
     7681 ctgcaggagc tgctgccggg caagccgatt cgcatcgcca accagcagat gtacaacctc
     7741 gtggccgcag gggcgctggt cgtgctgacc atcctgacgg tcagcctcga tcacaacacg
     7801 accacgaagg gcatgctttg ggtgatcttc gcgatcgcgc tggccctcgg tctctcggtc
     7861 gtgctgccga tcggtggcgc ggacatgccg gtcgtgatcg ccatcctcaa ctcgctgact
     7921 ggtctggccg cggcggtggc cggacttgcg ctcgataacc aggcgatggt tatcgccggt
     7981 gcgctggtcg gtgcatccgg ttcctacctg accgtcttga tggcacgggc catgaaccgc
     8041 tccgtgttta acgtcgtctt cggatcgtgg ggcgcaccag aagtcgccgg tccagccgcc
     8101 ggaagcggcg agcagcttcc gattcgcgag acctcggccg aggatgtcgc cgtccagctc
     8161 gcctacgcca acaaggtcat catcgtcccc ggttacggat tggcggtggc gcaggcccaa
     8221 cacacggtcc gcgagctcgc cgacgagctc caggaacgcg gcgtcgacgt caagtacgcg
     8281 atccacccgg tcgctggtcg catgccgggg cacatgaacg tgctcctggc cgaggccaac
     8341 gtaccgtacg aggatctgta cgagatggag cagatcaacg acgacttccc gagcaccgac
     8401 gtcgccctcg tcatcggcgc gaacgacgtg accaaccctg ccgcgcgaga cgacaagacg
     8461 agcccgatct acgggatgcc gatcctgaat gtcgacgaag cccagaacat cgtcgtgctc
     8521 aaacgctcga tgaagtccgg ctatgcgggc atcgagaaca tgctcttcca cgatccgaag
     8581 acaagcatgc tcttcggcga tgccaaaacc tcgctgtccg agctcgtcac cgcagtcaag
     8641 gaagcctgac agtagccaac tgcagacgcc gatcaaacgg gttctggaga atgttccaga
     8701 gcccgtttgt tttcaggttg caactcggct aatattccag ttctcggcgg actagtggtt
     8761 acaaccatcg cgaggtggca agtggcagaa caacgcggtt cggcacggta cgaggcgtcg
     8821 taccagacgg ttctcgacgc ggttgcgctc gttccatcag cgcaacggac aactccgggc
     8881 gtttgcggcg attggtcgct caaggatgtg ctcggtcacc ttgccttctg ggacggcgac
     8941 ctcgccgtcg agctcgaggc gaagaagaat ggcgtcaccc tcccgacctc gagcgagcgc
     9001 gagtggcaag cgatcaattc cgagcaagca gcgattcggg ccgaatggga ctgggatgat
     9061 gtcttgcgtg aggtcaggga gaatcacgac cgccttgtcc ccgtcctgaa cgatccgggc
     9121 gagaacccga atgacgagcc gatccatgag cactgggatg agcaccacgc gcaaatcgat
     9181 gcctggttga ccgccaacgg tcatgcgaag gacgacctcg cacccgagcg cagccgcgac
     9241 ctctacgagg agttgcgccg atcagccgtg caaaccgcgc tgggcgtgcc ggagacactg
     9301 tgcgacgagc ctggcgtttg cgggagctgg tcactgaagg agctgatggg ccacctggcg
     9361 ttctgggaca gccgcgtcgc cgatcgactc gaagcgcgga aagctggccg cacctggcag
     9421 cgcgactccc gcagctacga cgtcatcaat gccgaagccg ccgcggcccg atccacctgc
     9481 acgtgggacg agattcaggc cgagctggct gagacgcatg ctcgcctcct gcctctcctg
     9541 tccgaccccg gcgaagaggg cgactaccgc atctatatgc actggcgcga gcacggcgcg
     9601 cagatcgaag cctgggccgc cgcgcacagc cacgtctagc aagggaacaa gatgattcag
     9661 acggttcagc cggccccact aacactcgac gagacgatcg atcgcatcga agcagcgtgg
     9721 cgagattggc tcctggctct cgaagcagtc gatcccgccc ggcgcaccga gccgggggtc
     9781 tgcggctact ggtcggtcaa ggacatcatg gcccacatct cgctctggga aaacgagatc
     9841 cacgagcaca tccagcggtg gcagctcgga ctcccgaagt ccgacctcga tgtggaccag
     9901 atgaacgcgg cgatcgttgc ggagaaccac gtccggccgt atgcgctggt gcgagtcaat
     9961 atgcaccgcg cccaccacga cgcgatggca gcaatcaggg ccatcgaggg cgacctggat
    10021 gacgacattc gcgaccggat cgcctgcgag acgtgggatc actatcccga gcacaccgag
    10081 cagatcctca cctggctcaa gcggaaccag cctgatggct gaccgctggc tcgagctgct
    10141 cgactccgcc tggacccgct ggctggaggc gctggaccag gtcgatcccg ctcggcgaaa
    10201 tgaaaaggtg ctggatgaca actggtcgat caaggacctc gtcgctcaca ccctcttctg
    10261 ggatgcggaa gtgctcaccg acatccagcg ttggcagcat ggccgaccgc cgatgagcaa
    10321 cgactggcag cggatgaacg acgagaatca cgccgcgcac agaaatcggc cctacgatct
    10381 gcttcgagtc gaaatgcatc tcgtccacca gaccgtccgg gacgcgatcg ctcagctccc
    10441 tgccgacatc ccggaggaac tgatcgagcg ggtcgccgtc gatacctggg accactacga
    10501 cgaccacaca cagcagatcg aaggctggct cgcgaaataa ctccccgggc tatgatcgcg
    10561 gaatgaagcg gactcttgcc gtcatcgcga tcatcatcgt cgtcattgcg atcctgcggc
    10621 cgtcgaccat tattgatcgc tccggtcgat cccaaccgac tcccacttcg accgtcgtgc
    10681 aaaccggtgg cgttcgcggg ctggtcgtac ttcccgatga tggcccgaac gcgatcctcg
    10741 acgagatcaa ccaggcaaaa cgctcaattg atctttatgt ctatctcctc ccgtccgacc
    10801 aggtcctctc cgctctctca gcggcacatg accgcggggt cgcgatccgg gtcatcctcg
    10861 agcgcgatcc cttcggcggc ggcaactcca accaggacgc ctacgaccgc ttggcagccg
    10921 ccggaatcgc agtccgatgg tccagcgatc agttccagtt cagccacatc aagacgtttg
    10981 tggtcgatca gcgggtcgcg gtcgtgatga cgttgaatct ctcgtggtct gccctgactc
    11041 gcaaccgcga gttcgccgtc gtctcgacga tcccggccga cgtccatgag gtatcggggt
    11101 tgttcgaggc cgactggtct gcgcagccct actcccccga gggcgacttc gtggccagcc
    11161 cggagaacag ccgggccgtg ttccgcgggt tgatcgacaa cgccagcata tcgatcgaca
    11221 tctacgctga ggtggttcgc gacaccaaca tccgccagcg ccttgccaat gcggcggctc
    11281 gcggtgtgac cgtccgcatc cttgtgccgg ccaatccgac cgccgacgcg ctccagatct
    11341 accgggatct cgagcgcgat ggcgtgcagg tccggctcct cgccgatgcc tacagccacg
    11401 ccaaggcgat cattatcgat ggcacatcag ccttcgtcgg atcgcagaac ctgacccaga
    11461 cctcgctcga caagaatcgc gagctcggca tgatcctcac cgatcaaccc aatctcgatc
    11521 gcctcgccca tgtcttcgag tccgattgga gcatctcgcc agcggcctga acccaatcgg
    11581 caagaaccaa cgtaattggt aacgtccgcc cggcgccgcc aaacctgttg gcgtagcatc
    11641 ggatggttgc cgctcaattg atggtgccgg actaaaccgg cgatttgagc ggcggcgaag
    11701 tacccggcaa ctaaccacgc cgcgcgttcc ggaacaaccg gacaccggaa ggaggcgcga
    11761 tgtcgcagcg tcctcaccga atcgcgccct gattgacgca attcgcctga gtcacggtgg
    11821 atcgcttggg aaccggcgcg ttcccaagcc tgcaatgccg ccgagatcgc aacgatgccc
    11881 tctcgtgccc gtgagccacg tcagccgaag cccggctacc tgtattggca ctgatcaaca
    11941 cgacgcacta aacaggtcgg cgcagccgaa tctgtgacgg gcggtcgtgc cacgaggagg
    12001 aacccgatgc gcaacatcct cgcactctgc gcagccctgg cagttctgat tctcggtctc
    12061 ggtctggcaa cgggcgcccg gggccagaac gtctcctacg ccagccccac tccgggcgag
    12121 acctactgcg caaccccggt cgccagcatg gtcgggacgc cggccatcgt agttacggca
    12181 cccacaacgg cagcatcacc gggcggggtc gccccgggta cggccgtcgg actcgtcccg
    12241 tgcggcacac cgattggcac gcccgctgat ggcggtagcc aggcgcaaac cgcacccacc
    12301 atctcgatgg tggacatcgc ctacaacccg aatcagctca cgattcctgc taacaccgac
    12361 gtcacagtga cgctgaagaa caccggccag ttgccgcata ccttcgacat tgatgcgctg
    12421 aacatccact cggatcaagt ccagccgggc caaagcgcga ccgtcaagat caac
//
LOCUS       DATAOZ010000098         5406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5828368, whole genome shotgun sequence.
ACCESSION   DATAOZ010000098 DATAOZ010000000
VERSION     DATAOZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5828368"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1090)
                     /locus_tag="VHR64_02810"
     CDS             complement(167..1090)
                     /locus_tag="VHR64_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEX3301784.1"
                     /translation="MAIAQPSATQVRRANIWLATGQAVGLVLLALAAILLLVGIAGVS
                     LGEPDLAGRVLTLAAPALAAGMLSILSPCSLPIVLGYFSVMFQQQPGRIGAITLAFLL
                     GVGTTMSILGAGFTALGSLAIEHQEQMTRVGGLLIIGFGLLTLTGRGFSGMRMTGRSG
                     AGLGAAYLFGLMFALGWTACVGPILGSILTLLLADAASSSGAISLISGAALSQVYVLG
                     MGLPILVTVLGLLQGGSSREIGRSLRGRALSVRISHRQISLPPATLLSGVMLIGLGIL
                     MLNGTMTRLSQQLGSSALSQWVVRIEEMIPT"
     gene            complement(1090..1644)
                     /locus_tag="VHR64_02815"
     CDS             complement(1090..1644)
                     /locus_tag="VHR64_02815"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3301785.1"
                     /translation="MSSQRLMSARSRPLLLGGLLLGLAGCGGDDATSDAGVIPWPDKR
                     ATGAEPGNAAPNFGLQTPDSTRLELATLTGSQPLVVNFLATWCANCMEEMDILVDLHD
                     SGVPVLGVNLREEAETVRSLIERTAARFPILLDRTGKVTRAYKVVNLPATVVLDAQGR
                     IASVTRGPISATGIRDAVAAAGES"
     gene            complement(1646..2065)
                     /locus_tag="VHR64_02820"
     CDS             complement(1646..2065)
                     /locus_tag="VHR64_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301786.1"
                     /translation="MSQLVIVQLSPPGNRRSSPALKWIAGAIAGALLLIAAGIILLQQ
                     TAPNDDPGLLFEIPAGAAATVPAGLISAVDIPREIIFRDGDTAKITVINHDSVTHLAG
                     PFVVGPGETYVQTFPNRGNFPINCAVNPEESIVVKVE"
     gene            complement(2132..3205)
                     /locus_tag="VHR64_02825"
     CDS             complement(2132..3205)
                     /locus_tag="VHR64_02825"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX3301787.1"
                     /translation="MNRTISRRTLITSAAALSLAGIAGARSSAHQSPMSGTPGPMGSP
                     MPMGGTGAAFLKVANSGDSADRLVGAKTAAAKTVEIHEMRNEGDVMKMAPLADGLEIP
                     SGATVELKPGGFHIMMIGLTKNLKAGESFELTLTFEQAGEVALTVPIYVSKEAAEAAL
                     AQSQDKPVLAGDLAITGVWSRQAPAMMAESSPSPAPAGPPMGGFAGVGAAFMVITNSG
                     SDADLLLGASTDLAERVELDEIRIGSGGMAMIPLHDGLPIPAGETVTITPGKYHILLY
                     GLKRDLVAGESFDLTLSFEKAGEATFPVPIFASSVDAEAAASATPMQPATAGDLMITG
                     IWSAAAKASPDGTPVAAAMGANT"
     gene            complement(3228..3821)
                     /locus_tag="VHR64_02830"
     CDS             complement(3228..3821)
                     /locus_tag="VHR64_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX3301788.1"
                     /translation="MHRPRPRHALGMLVMVLLLSGCSLTSNYTFKTGALSPARDAPPL
                     PLTDQHGQPFDLANLRGKAVVLYFGYTACPDACPTTLSDWIEVKRLLGDKAEKVAFVM
                     VTVDPERDTPDQLNQYLTFFDPAFIGLTGDPETIRAVESDYGILAARDEFDDSAMGYM
                     MSHTTSYLAIDRDGKLRLVISHGTEPEIVAEDLRHLI"
     gene            complement(3919..4419)
                     /locus_tag="VHR64_02835"
     CDS             complement(3919..4419)
                     /locus_tag="VHR64_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301789.1"
                     /translation="MTAAPVWLQRTLSWIAPVLLAGMLLFPSTCAYAAGVHSLFDDPL
                     AAPMPPGHHPALARYAAQYGMTFAELERHVAMGHIVLPTDAIEQPPTEASVAVAEIDP
                     CPSAPKFRDMPSSMLMDNVAAVTTIADPDVISAPIARADVIWPMVQLPAGHLADVESP
                     PPRAVS"
     gene            complement(4546..>5406)
                     /locus_tag="VHR64_02840"
     CDS             complement(4546..>5406)
                     /locus_tag="VHR64_02840"
                     /inference="COORDINATES: protein motif:HMM:NF027968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3301790.1"
                     /translation="NELQALFSFPLNRFTFLGLAQKNADAIKFGLGQLPTIPDAGQWV
                     NFLRHHDELNLTQLTKEQRGEILDVFAPKPGMRIYNRGLRRRLAPMFDGDLARLRMAY
                     SLLLGPPGAPLIFYGEEIGMGENLRIPGRNSVRTPMQWTAHNYGGFSTAAPEDFVRPM
                     TGGEYGLNTINVGAQRSDPDALLNFMATLIRTRRECAEIGSGTGTSLDLGTDHVLGLR
                     HETEDSAVIVLNNLSDQAQEITVELTHHERGVVTELLQDSPYPPITETGGKMTIGPYG
                     YRWFRIGGIY"
BASE COUNT          923 a         1714 c         1853 g          916 t
ORIGIN      
        1 tggcaggcgg catttcgtca tgccgcctgc cacgctcggg gcgtagcgcc ggccccccgt
       61 ggccggctcg gggttgacgg cacgcacggt ggatagatcc gatcggacct ccccagcggc
      121 acacccggca ccgaagccgc ccagaggtgg ccgcgctacg gcggcctcag gtcgggatca
      181 tctcctcgat cctgacgacc cactgcgaca gagcgctcga tccgagctgc tggctgaggc
      241 gggtcatcgt gccgttcagc atcaggatgc ccagcccgat cagcatcacc ccactcagca
      301 acgtcgccgg cggcagcgag atctgccggt gactgatccg gacgctcagc gcgcgaccac
      361 gcagcgaccg cccgatctcc cggctgcttc ctccctggag caggccaagc actgtgacca
      421 gaatcggcaa ccccatcccc agcacataga cctgtgagag cgccgcgccc gagatcagcg
      481 agatcgcccc gctcgacgag gccgcatccg ccaatagcag ggtcaggatc gagccgagga
      541 tcggcccgac gcaggcggtc cagccgagcg cgaacatcag cccgaacagg taggctgccc
      601 caagacctgc tccgcttcta ccggtcatgc gcatgccgct gaagccccgg ccggtcaggg
      661 tcagcaggcc gaacccgatg atcagtaggc cgccaacccg ggtcatctgc tcctggtgct
      721 caatcgccag cgatccgagc gcggtgaagc cggcgccgag gatgctcatc gtcgttccca
      781 ccccgagcag aaaggccaga gtgatcgcgc cgatccggcc tggctgctgc tggaacatga
      841 ccgagaagta gccgagcacg atcggaagcg agcagggcga gaggatcgag agcatcccgg
      901 cggccagggc gggcgcggcc agtgtcaaca cgcgccccgc gaggtccggc tcaccgagcg
      961 agacgccggc aatcccgacc agcagcagga tcgccgccag tgccagcagg accaacccga
     1021 cggcctgtcc ggtcgcaagc cagatattgg cgcgccggac ctgagttgcg gatggttgcg
     1081 cgatggccat cagctctctc ccgctgctgc gacggcgtcg cgaatgccgg tcgcgctaat
     1141 cggtccgcgg gtgaccgatg caatccggcc ctgcgcgtcc agcacgacgg ttgccggcag
     1201 gttgaccacc ttgtaggcac gggtcacttt accggtgcgg tcgagcagga tcgggaagcg
     1261 ggcagccgtc cgctcgatca gggacctgac agtctccgct tcctcgcgga ggttgacccc
     1321 aagtaccggt acgccgctgt cgtgcaggtc gaccaggata tccatctcct ccatgcagtt
     1381 ggcgcaccag gtagccagga agttgaccac cagcggttgg ctgccggtca gtgtggccag
     1441 ctccagccgc gtgctatcgg gcgtctggag gccgaagttg ggcgcggcgt tgcccggctc
     1501 ggcgccagtg gctcgcttgt ccggccatgg gatgacgccc gcgtcggagg tcgcgtcatc
     1561 tccaccgcac ccggccagac caagcaggag gccaccgagc aggagcgggc gagaccgcgc
     1621 gctcatcaat cgctgggacg acatctcatt ccactttcac cacgatcgac tcctccgggt
     1681 tgacggcgca gttgatcggg aagttgcccc ggttcgggaa ggtttggacg taggtttccc
     1741 ccgggccgac cacgaacggg ccggccagat gggtgacgga gtcgtgattg atgacggtga
     1801 tcttcgccgt gtcgccgtcc cggaagatga tttcgcgcgg gatatcgacg gccgagatca
     1861 acccggctgg gacggtcgcg gcggcgccgg ccgggatctc gaagagcagg ccaggatcat
     1921 cattcggcgc ggtctgctgg agcaggatga taccggccgc gatcaggagt aaggccccgg
     1981 cgatcgcacc cgcgatccat ttcagcgcgg gtgacgagcg tcgattgccg ggaggagaga
     2041 gttgaacgat gacgagctgg ctcatggcga tctctctttc cgcgcaggtg catgcgtggg
     2101 gacggcgccg tccccacgct ctcagcgtct cctaggtgtt tgcgcccatt gccgccgcca
     2161 ccggggtccc gtctggcgat gccttggccg cggccgacca gattccggtg atcatcagat
     2221 cgcccgcggt cgcaggctgc atcggggtcg cgcttgcggc ggcctcggcg tcgacgcttg
     2281 aggcgaagat cggcaccggg aaggtcgcct cgcccgcctt ctcgaaggag agcgtcagat
     2341 cgaacgattc gccggcgacg aggtcgcgct tcaggccgta aagcaggatg tggtacttgc
     2401 cgggcgtgat cgtgacggtc tcgccggccg ggatcgggag cccgtcgtgg agcggaatca
     2461 tcgccatccc gccgctgccg atcctgatct cgtcgagctc gacccgctcg gcgagatcag
     2521 tcgatgcgcc aagcagcaga tcggcgtcgg atccgctgtt ggtgatcacc atgaaggccg
     2581 cgccgacccc ggcgaagcca cccatcggcg gtccggccgg cgccggtgac ggcgagcttt
     2641 ccgccatcat ggccggagcc tggcgggacc agacaccggt gatggccaga tcgcccgcga
     2701 ggaccggctt atcctggctt tgcgccagcg cggcttcggc agcctccttc gagacgtaga
     2761 tcgggaccgt gagcgcgacc tcgccggcct gctcgaatgt cagcgtcaac tcgaacgact
     2821 cgccggcctt caggttcttc gtcaggccga tcatcatgat gtggaagccg cccggcttga
     2881 gttcgaccgt cgcgccggac ggaatctcga gaccatcagc gagtggggcc atcttcatga
     2941 cgtcgccctc gttgcgcatt tcatgaatct cgacggtctt cgcggcagcg gtttttgcgc
     3001 caaccagccg gtcggcggaa tcaccggaat tcgcgacctt caggaaggcg gcgcccgtcc
     3061 cgcccattgg catcgggctg cccatcggac cgggtgttcc gctcatcggc gactgatggg
     3121 cgctcgaccg ggccccggca atcccggcca atgacagggc tgcggcggag gtgatcagag
     3181 ttcggcgtga gattgtgcgg ttcacgattg tcatttcctc aggtacttca aatcaagtgg
     3241 cgcaggtctt ctgcaacgat ttcgggctcg gttccatgcg agatcacgag gcgcaatttg
     3301 ccgtcgcgat cgatcgccag gtacgacgtg gtgtggctca tcatgtaacc catcgcggag
     3361 tcgtcgaact cgtcgcgagc ggccaagatg ccgtagtcgc tctcgacggc ccggatcgtt
     3421 tcgggatcgc cggtcagtcc gatgaatgcg gggtcgaaga aggtcaggta ctggttgagt
     3481 tggtcaggcg tgtcgcgctc ggggtcgacc gtgaccatga cgaaggcgac cttctcggcc
     3541 ttatcgccca gcagccgctt gacttcgatc cagtcgctca aagtggtcgg acaggcatcg
     3601 gggcaggcgg tgtagccgaa gtagaggacg accgccttgc ccctcaggtt ggccagatcg
     3661 aagggctggc cgtgctgatc ggtcagcggt aggggcggcg catcgcgcgc gggcgagagg
     3721 gcgccggtct tgaaggtgta gttgctggtc aggctgcagc cagagagcag caggaccata
     3781 acgagcatgc ccagggcatg gcgcggacgg ggccggtgca cggcgtcggt ctcctcgcaa
     3841 ggctgagttc ggactccggc gggcgtggct gcccggccgg atctcattcc gggttgatcg
     3901 aacggggtct gcggaatgtc aggagactgc gcgaggaggc ggactttcga cgtcggcgag
     3961 gtgaccagcc ggaagctgca ccatcggcca gatgacgtcg gcacgtgcga tcggcgcgga
     4021 gatcacgtcc gggtcggcaa tggtggtcac cgctgcgacg ttgtccatca gcatggacga
     4081 gggcatgtcg cggaatttcg gcgcgctcgg gcacgggtcg atctcggcga ccgccacgga
     4141 cgcttccgtg ggtggctgct cgatcgcatc ggtcggcaga acgatgtggc ccatggcgac
     4201 atgtcgctcg agctcggcga aggtcatccc gtattgcgcg gcgtaccggg cgagggcggg
     4261 atgatggccc ggcggcatcg gcgcggcgag gggatcatcg aagagggaat ggactccggc
     4321 ggcatatgca caggtcgagg ggaagaggag catgcccgcg agcagtaccg gcgcaatcca
     4381 tgaaagtgtt cgctggagcc aaaccggcgc cgcagtcacg gtgccctccg ttcgactgaa
     4441 ccgcttcagt caaacctgat ggtaccagcc ggggagtgcc tgtgaggcga ttttcgggcg
     4501 gtgttgcggt tttgtaacgc gggtggagtc acagagccgg gaacatcaat agatcccgcc
     4561 gatgcggaac caacggtacc catacggccc gatcgtcatc ttcccgccgg tctcggtgat
     4621 cggaggatat gggctgtcct ggagcagctc ggtgacgact ccgcgctcgt ggtgggtcaa
     4681 ctcgaccgtg atctcctgcg cctgatcgga gaggttattg agcacgatga ccgcgctgtc
     4741 ctccgtctcg tgacggagac cgagcacatg gtctgttccg agatcgagcg acgtgcccgt
     4801 gccgcttccg atctccgcgc actcgcgccg ggtccggatc aacgtcgcca tgaaattgag
     4861 caacgcatcc ggatcggacc gctgggcgcc gacattgatg gtgttcaggc cgtattcgcc
     4921 accggtcatc gggcggacga aatcttcggg cgcggcggtc gagaatcccc cgtagttgtg
     4981 cgcggtccat tgcatcggcg tgcgcacact gttccggccc gggatccgca ggttttcgcc
     5041 catgccgatc tcctcgccgt agaagatcag cggggcgccg ggcgggccca agaggaggct
     5101 gtaggccatg cgcaggcgag ccaaatcgcc gtcgaacatc ggggccaggc ggcggcggag
     5161 gccgcggttg taaatccgca tgcccggctt gggcgcgaag acgtcgagga tctcgccgcg
     5221 ttgctccttc gtgagctgcg ttaggttgag ctcatcatga tgccgcagga agttgaccca
     5281 ttgcccagcg tcggggattg tcggcagctg gccgagaccg aacttgatcg cgtcggcatt
     5341 cttctgggcc aaaccgagga acgtgaaacg attcagcgga aagctgaaga gcgcctgcag
     5401 ctcgtt
//
LOCUS       DATAOZ010000099         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5038837, whole genome shotgun sequence.
ACCESSION   DATAOZ010000099 DATAOZ010000000
VERSION     DATAOZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5038837"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..410
                     /locus_tag="VHR64_02845"
     CDS             90..410
                     /locus_tag="VHR64_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3301791.1"
                     /translation="MTAVNIPGLLTVADAANELSRSTEQVRRYLREGRLRGRRFGGQW
                     FIEEAALSAFRESLNDQSSYLDKLRPAGQIQALDGVIGIGHGGGSNIAEGKDSYRRSS
                     WWRR"
     gene            432..833
                     /locus_tag="VHR64_02850"
     CDS             432..833
                     /locus_tag="VHR64_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX3301792.1"
                     /translation="MASEDRPVAFVDSSAIVALVDQDDAAHSAAADAYHELCSSNYRL
                     FTTDHVVVEAYDLLLNGVGPVVAATWLRNHRLPVYCTDETDLVAAQTILSGRTADSPV
                     SFTDALSLAVMERLGVADAFAVDPAFLDALS"
     gene            966..1760
                     /locus_tag="VHR64_02855"
     CDS             966..1760
                     /locus_tag="VHR64_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301793.1"
                     /translation="MFEDILPIASYWDQPNLPGRAVAQQVTEADPHESVFTAENDDVS
                     DVPATLPYSPVVDPWSSAGGRLAAEPGSAQAFVPPSQSAGRHGQGGPPAWLLGTAGLL
                     LILGVAAAALLVVVRPLVSDRIESATGDAIAIALSQTTLAPEIGSGTVVISERQINRS
                     IRARRADYQPVEDLRVQIRRSGIQATFSVYGTSATLTGTVTVRNGRIVIVNPSLSGTA
                     GRIVSVDRVAADAERAINDYLKRNNLKPTAVTLSDDTLTITTVPIG"
     gene            complement(1823..>3062)
                     /locus_tag="VHR64_02860"
     CDS             complement(1823..>3062)
                     /locus_tag="VHR64_02860"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX3301794.1"
                     /translation="RHSMVRVRGRPMAEWRNWPGNVVATPARIERPGSIEAVQELVVD
                     AARAGLSVRVSGAGHSFTPICVTDGLLLDLTDLSGVIALDAEQQTATILGGTRIHQIG
                     APLFEAGLAVANQGDIDVQAIAGAIGTGTHGTGRRFGSFSSTLVGCQIVTANGDLLTI
                     DEADLETLRAARVSLGTLGVMVSVTLGLVPAYKLMRRSWPVAWSDALAQWPEVEANSR
                     NPEFWWIPPLDTSVFKVFVESEEEPTGTPPAPTFPPGTIERYLPPDGVDWAWRVYPAV
                     REHRFVEMEYALPIERGLEAIASIRNLMRSRHPEVRWAVEFRTHAGEDAFLSVTQGGD
                     SVTISVHDAADAPHWDFFREAEQVFRSFDGRPHWGKLNFLETSELRSLFPLLDRFTAV
                     RRHLDPEGIFLNDYLRPILA"
BASE COUNT          556 a          993 c          977 g          536 t
ORIGIN      
        1 cgaaagtcat ggtcgccgat cttgagcgtc gtgttgtcgt gtgctacgct gaacgaaact
       61 gtacgtacgc ttcagatgag ggttgccaga tgacagccgt caacatcccc ggattgctga
      121 ccgttgcgga cgcggcgaac gaactcagtc gctcgaccga gcaggtgcga cgctacctgc
      181 gcgagggccg cctccgtggc cgcaggttcg gcgggcagtg gttcatcgaa gaggctgccc
      241 tgtcagcgtt ccgcgaatcg ctcaacgacc agagctcgta cctcgacaag ctccggccgg
      301 ccggccagat ccaagcactg gatggggtca tcgggatcgg tcacggcggg ggcagcaata
      361 ttgccgaggg gaaggattcg tatcgccggt cgtcctggtg gcggcggtaa cggaagaggg
      421 agcaactgca aatggcatct gaagatcggc cagtcgcctt tgtcgattct tccgcgattg
      481 tcgctcttgt tgaccaggac gacgcggcgc attctgccgc agccgacgcc taccacgaac
      541 tctgctcgtc caactatcgg ctgttcacga cagaccacgt tgttgttgag gcgtacgacc
      601 tgctgctaaa tggcgtcggc cctgttgtcg cggccacctg gctgcgcaat caccggctgc
      661 ccgtctattg cactgacgaa accgacctgg tggcggcaca gacgatcctg agcggccgga
      721 cggcggactc cccggtgtca ttcaccgatg cgctcagcct ggcggtgatg gagcggctcg
      781 gcgtcgcgga cgcgtttgcg gtcgatccgg cattcctgga tgcgctgagc tagggcaata
      841 tgggccgatt accgatgcaa cacccggcgg ccaccggcca ggcgcaggtg accaggccaa
      901 ggttctgtcc acgatgcggt tcggcgacca cggccattgg gctgcgttgc actgtgtgcg
      961 gcctcatgtt cgaggacatc ctcccgatcg cgagttactg ggatcagccc aacctgcccg
     1021 gccgtgcggt cgcccaacag gttactgagg ctgatccgca tgagagcgtg ttcacagccg
     1081 aaaatgacga cgtctccgat gtgcccgcca ccttgccgta ttcgccagtg gtggatccgt
     1141 ggtcatccgc cggaggtcgt ttggctgctg agccgggatc ggcccaggca ttcgtgccac
     1201 cgagccaatc ggctgggcgt catggacaag gcgggccacc ggcatggctg cttggcaccg
     1261 ccggactgct gctgatcctc ggcgtcgcgg cggctgcact cctcgtcgta gtccgcccgc
     1321 tggtatccga tcggatcgag tcggcaaccg gcgatgcgat cgccatcgcc ctgtcccaaa
     1381 ccaccctcgc gcccgagatc gggtccggaa cggtggtgat cagcgagcgg cagatcaacc
     1441 ggtcgatccg ggctcgtcgc gccgactatc agccagttga ggacctgaga gtccagatcc
     1501 ggcggagtgg gatccaggcg acgttttcgg tatacggaac gtcggcgacc ctgacaggta
     1561 cggtaacggt caggaacgga aggatcgtga tcgtcaatcc gtcgctcagc ggaaccgccg
     1621 ggcgcattgt ctcggtcgac cgtgtcgccg ccgacgccga gcgcgccatc aacgactacc
     1681 tcaagcgcaa caatctgaag ccaactgccg ttacgctctc cgacgacacg ctgacgatca
     1741 cgacggtacc gatcggttga gggttgaggg aacgggaaag tagctagtag cctgcacgct
     1801 ctgttccaaa gaccaaagac catcacgcca gaatcggccg caggtagtcg ttcaggaaga
     1861 tgccttcggg gtcgaggtgt cgacggactg cggtgaaccg gtcgaggagc gggaagaggg
     1921 agcgcaattc gctagtttcg aggaagttga gcttgcccca gtgtgggcgg ccatcgaagc
     1981 tccgaaagac ctgctccgcc tcccggaaga agtcccagtg cggagcgtcc gccgcgtcgt
     2041 ggacgctgat cgtgaccgag tccccgccct gcgttacgct caggaatgcg tcctcgccgg
     2101 cgtgggtccg gaattcgacc gcccagcgca cctcgggatg ccgcgaccgc atgagattcc
     2161 gaatcgacgc aatcgcctcg agaccccgct cgattggtaa cgcgtactcc atctccacga
     2221 atcgatgctc gcggacggcc gggtagaccc gccatgccca gtcaactcca tctggcggca
     2281 ggtagcgctc gatcgtgccc ggcgggaagg ttggcgcggg cggcgtgccg gtcggttcct
     2341 cttcggactc gacgaacacc ttgaagacgc tcgtgtccag cggcgggatc caccagaact
     2401 cgggattgcg tgaattggct tcgacttctg gccattgtgc gagtgcgtcg ctccaagcaa
     2461 cgggccagct ccgccgcatc aacttgtacg ccggaaccag cccgagcgtg accgaaacca
     2521 tcacgccgag cgtcccgagc gagacccgcg ccgcccgcag cgtttcgagg tccgcttcgt
     2581 cgatcgtcag cagatcgccg ttcgcggtca cgatctggca accgaccaac gttgaggaga
     2641 aggagccgaa tcgtcgccct gtcccgtggg tgccggtccc aatcgcgccg gcgatcgcct
     2701 ggacatcgat atcgccctgg ttggcaaccg ccaatcccgc ctcgaaaaga ggtgcgccta
     2761 tctggtgaat ccgcgtgcct ccgagaatcg tcgcggtctg ctgctcagca tcgagggcga
     2821 tcacgccgga gagatcggtc aagtcgagca gcagaccgtc cgtcacgcag atcggagtga
     2881 aggaatgccc cgcgccggag acccgcacgg acaggccggc ccgcgctgca tcaacgacga
     2941 gctcctgcac cgcctcgatc gatcccggcc gctcgatccg cgccggcgtc gccacgacat
     3001 ttcccggcca attccgccac tcagccatcg gcctgcccct cacgcgaacc atcgagtggc
     3061 gg
//
LOCUS       DATAOZ010000100         4346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6092314, whole genome shotgun sequence.
ACCESSION   DATAOZ010000100 DATAOZ010000000
VERSION     DATAOZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6092314"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /gene="rpiA"
                     /locus_tag="VHR64_02865"
     CDS             <1..648
                     /gene="rpiA"
                     /locus_tag="VHR64_02865"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HEX3301795.1"
                     /translation="AQELPSVGVIGLGSGTTAEAVVRALGERIAGEPGFSAIGVATST
                     RTARLAESVGIQICDLSDVDSIDIGFDGADEIDPELNLVKGRGGALLYEKLVAEICRD
                     YMVVSTDEKLVARLGTRLPLPVEVVPYGWVHTLHRLADLGLTPELRGGKAEPFRTDAG
                     NVILDCTPEQGLDLLALAPEIKAVSGVVEHGIFPGLARRAAIVDAGGHVEFLLRA"
     gene            complement(664..1176)
                     /locus_tag="VHR64_02870"
     CDS             complement(664..1176)
                     /locus_tag="VHR64_02870"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX3301796.1"
                     /translation="MPDRTPTNTKNLDRYGSDAIPWSRAHDELAKGESVFFVGTVDSD
                     NRPHVAGVGVAWFDGDLYFTSNLGSLKARNLAANPAATMAASLAGIDLTLEGTVEQVG
                     DTAILERIAGIYRNGNWPAEVAGDALTAPYSAPSAGPPPWHVFRFTFDTVVGVATAEP
                     YGATKWQFAR"
     gene            complement(1275..1550)
                     /locus_tag="VHR64_02875"
     CDS             complement(1275..1550)
                     /locus_tag="VHR64_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3301797.1"
                     /translation="MAIYAVQITYADRAARDRARPNHRAYLQSQLEAGKLVKSGPYVD
                     DSGALLIYETESEAELQEILSHDPYWTTKGCLGDVSIKEWTILFDRG"
     gene            1630..4251
                     /gene="mutS"
                     /locus_tag="VHR64_02880"
     CDS             1630..4251
                     /gene="mutS"
                     /locus_tag="VHR64_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX3301798.1"
                     /translation="MREADEPRVVPLRRQYLDIKARFPDTILLFRLGDFYETFEEDAR
                     IAADVLDIVLTSREMGKGQRVPLAGIPYHAAEGYIARLIAAGHKVAICEQVGEVTRGK
                     GLVERDVTRVVTPGTVDEPAMLDARRNNYIAGLIVEGTRVGLAAADITTGEFIATEIG
                     GQTIADALMTVGRELLRLQPAEIVRPAGTSGDPLPVPEVAWLPESATRSESDSWRWRQ
                     DRAEEVLQRHFGVGSLGGFGLGDKPLAARAAGGLLTYLLDTQLTGLKQITSLRTYSTD
                     AYMTLDLQTRRNLELLESARGEKKHSLIAVLDLTRTPMGARMLRHWLNQPLLQLDELK
                     LRQRDVRAFHQAALPRADLRTRLGTIGDIQRMANRAITGTMSPRELIQLRQSLAAIPG
                     LAGPAATLDSAPGIPELSDIASLLKSAVVDDPPAVLGRGQTIRSGFSAELDEHQRRAR
                     EAREWIAGLERTERDRTGIRSLKVGYNKVFGYYLEVTTAALATAERDRGEVLPADYIP
                     KQSLTNATRYFTPQLKEYETVVLTAEDTLNQLEGDIYRRVVGQVAAASGRLLAAAEAI
                     AYLDVVATLAEIAIQRHYVEPELTHDDMIEIEAGRHPTLEALVPRGEYVPNDACLTDR
                     QRIVILTGPNMAGKSSWLRQVALIVLLAQIGSFVPADRARIGLVDRIFTRIGAQDDIA
                     GGQSTFMVEMVETAAILNHATTRSLVVLDEIGRGTSTYDGLAIAQAIVEYIHNAPDLR
                     CKTLFATHYHELTHLEAVLPHVCCSRMDVLEDGDRIVFLHRVVPGGADRSYGIHVADL
                     AGIPKPVVDRARALLAELESTKSDSPRRTKEPVAQLRFIEEPNPALDRLAALNVDHLT
                     PVEAITRLYELKSLLDN"
BASE COUNT          754 a         1452 c         1384 g          756 t
ORIGIN      
        1 gcgcaggagt tgccgagcgt cggcgtgatc ggcctcggct ccggcaccac ggccgaagcc
       61 gtcgtgcggg cgctcggcga gcggattgca ggcgagccgg gtttcagcgc gatcggcgtc
      121 gccacatcca cccggactgc ccggctggcc gagtcggtcg gtatccagat ctgcgacttg
      181 tcggatgttg attcgattga tatcggcttc gatggcgccg acgagatcga tccggagctg
      241 aatctggtca agggacgcgg tggcgccctg ctctacgaga agctggtcgc cgagatctgc
      301 cgggactaca tggtcgtctc tacggatgag aagctcgtcg cccgcctcgg cacccgcttg
      361 ccgctaccgg tcgaggtggt gccatatggc tgggtccaca cactccatcg actggctgac
      421 ctgggtctga cgcccgagtt gcgcggcggc aaggccgagc cgttccggac cgacgccggc
      481 aacgtgatcc tcgactgcac accggaacag ggcctggacc tgctcgcgct ggcgccagag
      541 atcaaggccg tctccggtgt ggtcgaacac ggaatcttcc ctggcctcgc gcgccgcgct
      601 gcgatcgtcg acgccggtgg tcacgtcgaa ttcctgctca gggcatagcc cggacgcgag
      661 caatcagcgc gcgaattgcc atttggtcgc gccgtatggc tcggcagtcg cgaccccgac
      721 gaccgtgtcg aacgtgaagc ggaacacgtg ccacggagga ggccccgcac tcggcgcgct
      781 gtacggagcg gtcagggcgt cacccgcgac ctcggcgggc cagttgccat tacggtatat
      841 cccggcaatc cgttccagga tggccgtatc gccgacctgc tcgacggtcc cttcaagggt
      901 gagatcaatg ccagcgaggc ttgcggccat cgtagcggcc gggtttgccg ccagattacg
      961 ggccttgagc gagcccaggt tactagtgaa gtacaggtcg ccatcgaacc aggcgactcc
     1021 cactccggca acgtgcgggc ggttgtccga atccacggtg ccaacgaaga agacggactc
     1081 tcccttcgcg agctcgtcgt gagcccggct ccatggaatg gcatctgagc cgtaccggtc
     1141 gagattcttc gtgttggttg gcgttcgatc gggcatcggt ttgcctcctc ttgctccgca
     1201 atcgcagcag agccagtctc cgccgctatg atctggtcga tcgacgcgcg gtgaaatcga
     1261 cgcatcaggc atgcctagcc gcggtcgaag aggatcgtcc actccttgat cgagacatcg
     1321 cccaggcagc ccttcgtcgt ccagtacgga tcgtgagaga ggatctcctg aagttccgcc
     1381 tctgattctg tttcgtaaat cagcagtgct ccggagtcat ccacgtacgg gccgctcttg
     1441 accaactttc cggcctccaa ttgcgattgc aaatacgccc ggtggttcgg ccgtgcccgg
     1501 tcccgcgcgg ctcggtcggc atatgtgatt tgcactgcgt agatcgccat caagcaattc
     1561 tccccttctc tgttgtctcg aagcccaagt agccgttagt ctaccgatat ggcacgaatc
     1621 agatccgcga tgcgtgaggc cgatgagccc cgcgtcgtac cgctgcgacg gcaatacctg
     1681 gatatcaagg ctcggttccc ggacacgatc ctgctcttcc ggctcggcga tttctacgag
     1741 accttcgagg aggatgcccg gatcgccgcc gatgtgctcg acatcgtcct gaccagccgg
     1801 gagatgggca agggccagcg cgttccgctg gccggcatcc cctatcacgc cgccgagggc
     1861 tacatcgccc gcttgatcgc cgccggccac aaggtcgcga tttgcgagca ggtcggcgag
     1921 gtcacgaggg gcaaggggct ggtcgaacgc gacgtgaccc gcgtcgtcac gccgggaaca
     1981 gtcgacgagc cggctatgct cgatgcccgc cgcaacaact acatcgccgg cctgattgtg
     2041 gaaggcacgc gcgtcggcct ggccgcggcc gatatcacga cgggggagtt catcgcgacc
     2101 gagatcggcg gccagacgat cgccgacgcg ctgatgacag tcggccgcga gctgctccgg
     2161 ttgcagccgg ccgagatcgt ccgcccggcc ggcacgagcg gcgacccctt gccggtcccg
     2221 gaggtcgcct ggctccctga gtcagcaacg cgatccgagt cggacagttg gcgatggcgt
     2281 caggatcgcg ccgaagaagt ccttcagcgc cacttcggcg ttggatcatt aggcggtttc
     2341 ggtctcggcg acaagccgct ggccgcccgc gcagccggtg gcctgctcac ctacctgctc
     2401 gacacgcagc tgaccggcct gaagcagatc acatcgctcc gcacctattc gaccgacgcc
     2461 tacatgacgc tcgatctgca gacgcggcgc aacctcgaat tgctggaaag cgcgcgaggc
     2521 gagaagaagc acagcctgat cgcggtcctc gacctgaccc ggacaccgat gggcgcgcgc
     2581 atgctccggc attggctcaa tcaaccgcta ctccagctcg acgaactcaa gctccgccag
     2641 cgagacgtgc gcgccttcca tcaggccgcg ctgccccgtg ccgatctgcg cacgcggctc
     2701 ggcacgatcg gggacatcca gcggatggcg aatcgggcga tcaccggaac gatgtccccg
     2761 cgcgaactga tccagcttcg gcaatcgctg gcggcgatac cgggactggc cggaccggcc
     2821 gccacgctcg actccgcgcc gggcatcccg gagctctccg acatcgcgtc gttgctgaaa
     2881 tcggcggtgg tcgacgatcc gccggcggtg ctcgggcgag ggcagacgat tcgcagcgga
     2941 ttctcggccg agcttgatga gcatcagcgt cgcgcccgcg aggctcgcga gtggatcgcc
     3001 gggctcgagc gaaccgagcg cgatcggacc gggattcgct cgctcaaggt cggctacaac
     3061 aaggtcttcg gctactacct cgaggtaacg acagcagcgc tggcgactgc tgagcgggat
     3121 cgtggcgagg tcctgcctgc ggattacatc ccgaagcagt cgctgaccaa cgccacccgc
     3181 tatttcacgc cgcagctcaa ggagtacgag acggtcgtcc tgaccgccga ggacacgctc
     3241 aaccagctcg aaggcgacat ttatcgccgt gtcgtcggtc aggtcgcggc cgcttcgggg
     3301 cgattgctcg cagcggccga ggcgatcgcg tacctcgacg tcgtcgccac gctggccgag
     3361 atcgccatcc agcgccacta cgtcgagccg gagctgacgc acgacgacat gatcgaaatc
     3421 gaggccggcc gccacccgac cctcgaggcg ctcgtgcctc gcggcgagta cgtgccgaac
     3481 gatgcgtgtc tgactgatcg gcagcggatc gtgatcctga ccgggccgaa catggccggc
     3541 aagagcagct ggctacggca ggtcgcgctg atcgtgctcc tggcccagat cggctcgttc
     3601 gtccccgctg accgggcccg gatcgggctg gtcgaccgca tcttcacgcg gatcggcgcg
     3661 caggacgaca tcgccggcgg gcagagcacc ttcatggtcg agatggtcga gacggccgcg
     3721 atcctgaacc atgcgacaac tcggagcctg gtcgtactcg acgagatcgg gcgcggcacc
     3781 agcacgtatg acggtctggc gatcgcgcag gcaatcgtcg aatacatcca caacgcgcct
     3841 gacctgcgct gcaagacgct cttcgccacc cactatcacg aactgacgca cctggaagcc
     3901 gtcctgccgc acgtctgctg ctcgcgtatg gatgtacttg aggacggcga tcggatcgtc
     3961 ttcctgcatc gggtcgtgcc gggcggcgcc gatcgctcct acggcatcca tgtggccgat
     4021 ctggcgggca tccccaaacc ggtcgtcgat cgcgccaggg cgctcctggc cgagctggaa
     4081 tcgaccaaat ccgattcgcc gcgccgaacc aaagagccgg tcgcgcagct gcggttcatc
     4141 gaagagccga atccggcgct ggatcgactc gccgcgctca acgtcgacca cctcacgccg
     4201 gtcgaagcga ttacccgtct ctacgagttg aagagcctgc tcgacaatta ggacctcctc
     4261 gttcgcaact ttttcacatt acgacttgat atgtcggcta cactggcaga tggccgggcc
     4321 ccgcgagcgt gcccgtgtgc catttt
//
LOCUS       DATAOZ010000101         8505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_568324, whole genome shotgun sequence.
ACCESSION   DATAOZ010000101 DATAOZ010000000
VERSION     DATAOZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568324"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1143)
                     /locus_tag="VHR64_02885"
     CDS             complement(124..1143)
                     /locus_tag="VHR64_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301799.1"
                     /translation="MAIRTRAGDRDNLQSQGAVPEKRSTLARKVSERFRREDPHYIRH
                     FLSVSVARASSRLARALPTPVRYWIADRLGDLLYVLSPGYRRNVMSNLRHVHAGSGRA
                     EPTVRDVHNVFRVSARNWADLLVVPKRSQESFLAEFTLTPGSREHMDAAIARGKGCIL
                     ITAHVGAFDSVGHFLHALGYKLTIVTGRTTARIVFDGVTYLRQSNGLPLVEATPSGVR
                     RAIQAVMQGECAVIVSDRDFFQNGIRVEFFGQETTLPPGAVRIARDTGAAIVPVYGKR
                     VPGGHEVRIDEHFIVDRTTDLQRDLNAGMAPVVESLESALAETPDQWVMFQSVWPLEP
                     DGHDA"
     gene            complement(1170..1835)
                     /locus_tag="VHR64_02890"
     CDS             complement(1170..1835)
                     /locus_tag="VHR64_02890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX3301800.1"
                     /translation="MANLITIARLILLFVAIGMIYWGSAAWIAVAIVVIAIVFAGDGL
                     DGYIARRTNSTSQFGAVFDIAGDRIVENALWVIFAELQLIPVWIPLVVLTRGFIVDGI
                     RSMSYAEGMTAFGERNMMRSEFTRWLTAGRFMRTLFGWTKAFGFLFLTGLEGYRHRDA
                     PDSLIHQIYSWDPLRWAGWACVVVSVTLTVVRGLPVIYDATALWNKPATPAPEAVHES
                     TTG"
     gene            complement(1882..2370)
                     /locus_tag="VHR64_02895"
     CDS             complement(1882..2370)
                     /locus_tag="VHR64_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301801.1"
                     /translation="MQSELDIEYADEGYDDPDIDAIAPAGRARRPVARREMPGIPQQR
                     IREMSAIFANPSPELRPIVIGGVVALASLVLMSIMILIRSGSITDYIPLHVDAEGTTT
                     MFGTKGSIWRLPFFALLSTIMALGLGWWLRTRDAYAVQFLVVGALMVHCLIWVGAINL
                     LW"
     gene            complement(2863..4653)
                     /locus_tag="VHR64_02900"
     CDS             complement(2863..4653)
                     /locus_tag="VHR64_02900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX3301802.1"
                     /translation="MSQRRRDTLPKKRSGAAGPASQRRISRREREERQRRQLYIGLAI
                     AGVLSLVILAGFALNEYFLKPRAVLASVNGVEIRRRDYWKVRSVELVNQVNQYNQFSQ
                     LVDPSQQQQYLTLAQQAASELDKVWGSTDINDSTLTRMVEDQVYLQSLDDMGLSVTDQ
                     DVQNYLWQQFEPQNAPIFTPTPSPTLIPQRAEWATQTEVALQAENQTPTPVSAVGSPA
                     AGGGTPIAGQATPIGSPVAGTPIGSPVAGTPAASPISGTPIVIVIPSTAASPVAQASP
                     SGSAATPLAVQGTPGTLVGSPAASPVGSPVNQATPITQESATPEAATPNPTEAIATSE
                     ASYGNYKSAVFDIAHMSDSDYENLIVRPAIAREKIEGQLLKDVGQTAPQVHAAHILVD
                     TKDLADSIYAELQGGANFEETARQQSNDTSTAENGGDLGWFTRGQMVKPFEDVAFSLQ
                     PGQISQPFQTQYGWHIVKVYEVDQNRPMTDQQLQQYRDSIVSRWLEEQKTGMDISSKV
                     EPTPTPAVSSFVPPPDAPPLPTETPLPPEASPVTESGTPTEEQALPIGSPVASPIASP
                     MASPVGSPNASPVASPIASPTAMASATATP"
     gene            complement(4753..5181)
                     /gene="ssb"
                     /locus_tag="VHR64_02905"
     CDS             complement(4753..5181)
                     /gene="ssb"
                     /locus_tag="VHR64_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX3301803.1"
                     /translation="MYAKVQIIGNLGQDPETRYTKSGAMNVNFSVATSNRFTDASGTQ
                     QERTTWFRVTAWSKLAERLDRFAQEGVLAKGQRVFVSGRIEQREYTNQQGETRFSLDV
                     SADEVLLMSARGEGGGQGGYGGGRSAEPAAVAEDIDDLPF"
     gene            complement(5211..5744)
                     /gene="rpsF"
                     /locus_tag="VHR64_02910"
     CDS             complement(5211..5744)
                     /gene="rpsF"
                     /locus_tag="VHR64_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX3301804.1"
                     /translation="MSTDFKRTRDYELMVIFHPELSEEDLVSEGGKVEAHITGAAGAI
                     RLVNREAPWGRRRLAYPIRHGSRDIRDGVYVLYYFTAESGKIEEIEREIKLQDRVIRY
                     LLTQQTTPLMEPTPAEGEVAEEAAEGEATEAAQPAATVSTAEGEAATETAEQATETAE
                     PATETAEQASDTEAPVE"
     gene            complement(5880..7073)
                     /gene="cysS"
                     /locus_tag="VHR64_02915"
     CDS             complement(5880..7073)
                     /gene="cysS"
                     /locus_tag="VHR64_02915"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX3301805.1"
                     /translation="MNLYNSATRSVEPLTVIDNHVGIYVCGVTPYDTTHAGHAFTFLT
                     FDILVRFLRSRGYKVTYAQNVTDIDDDILRKSKELGTTWDELGRSETDKYLNDMRQLN
                     ALPFDHYLRATETVPLMVSLIETLVEEGHAYVANGSVYFSVSSDPEFGKLSRIPRGEM
                     LAVANERGNNPDDPNKQDPIDFVLWQAAKPGEPTWDSPWGPGRPGWHIECSAMSMSVL
                     GNTVDIHGGGADLIFPHHECEIAQSENATGVRPFTRYWMHVAMVEYQGEKMSKSLGNL
                     VIASNVLADYPADAFRLYLFSHHYRTPWEYIDEEIDGFAELARDMCEAIDTPSYSIGE
                     MLDSSRYRKRFIAAMENDLDTPAAIEQLRLLTSEILSAEDTDISQAKRDLRELAGILG
                     LTLSD"
     gene            7244..7879
                     /locus_tag="VHR64_02920"
     CDS             7244..7879
                     /locus_tag="VHR64_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301806.1"
                     /translation="MTVMLLAACGGPDLGNPADHTPIPSSTPTIARPTAAVGRTTGAP
                     CESGTLRIKDLPEIQKRWQSGLSVARGRAAAWKEDAVLIELGISCELFESGFRWQATF
                     FSRDAQAYYRSDTTEVIPVNTDPAAIIPLPDSEINFDGLYAVLMQSDKVDANGDDTIT
                     ALDVRVSTELQPIGPPGVPTGAAIYHVALRSHGLVIEMYIDAVQGQIYQFQ"
     gene            7888..>8505
                     /gene="otsB"
                     /locus_tag="VHR64_02925"
     CDS             7888..>8505
                     /gene="otsB"
                     /locus_tag="VHR64_02925"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX3301807.1"
                     /translation="MEVAERLLELAVMGRMALYTDIDGTISPIAATPSDAFLLPGAAE
                     ALSMICAHGIRVVAISGRAAADARRLVGLPELDYAGNHGFELMTAKGHVIAEDVAAAS
                     TSVKAALGDLIALSDALPTGLVIEDKTFTGSVHYRLTKNPDASAIVLKAVLSDIAARN
                     GLLLTEGRLVYELRPRLDINKGVFVMNDVRLQGIRTAAFLGDDITD"
BASE COUNT         1585 a         2627 c         2670 g         1623 t
ORIGIN      
        1 cggaagtcac gatcaccgac gccggcccgt acgatctctg gggcgccgcg gcatcagacg
       61 gcggccggat cgatcgggga ccggttcatg aagtgcgtcg gcgcccagcg aatcgtccaa
      121 aggtcaggcg tcgtggccgt ccggttccaa tggccacact gactggaaca tgacccactg
      181 atcgggcgtc tcggcgagcg cgctttccag ggactccaca actggcgcca taccggcgtt
      241 gagatcgcgc tggagatctg tggttcggtc gacgatgaag tgctcgtcga ttcggacttc
      301 gtggccaccc gggacccgct tgccgtagac cggcacgatt gccgctccgg tatcgcgcgc
      361 gatgcggacc gcacccggcg gaagcgtcgt ctcctgcccg aagaattcga cccgaatgcc
      421 attctgaaag aaatcgcgat cggacacgat cacggcgcac tcgccctgca tcacggcctg
      481 gatggcgcgt cgcaccccgg agggtgtcgc ctcaacgagc ggcaggccgt tagattggcg
      541 aaggtacgtc acgccgtcga agacgatcct ggccgtcgtg cggccggtca cgatcgtgag
      601 cttgtacccg agcgcgtgca ggaagtggcc aacggagtcg aacgcgccaa catgggccgt
      661 gatcaggatg cagcccttgc cacgggcgat tgccgcatcc atgtgctcac gcgagccggg
      721 agtcagtgtg aattcagcaa gaaagctttc ctgacttcgt ttcggcacga ccagaagatc
      781 agcccagttc cgggcagaga cccgaaatac gttgtggaca tcacgcacgg tcggctccgc
      841 gcgaccactg cctgcgtgga cgtggcgcag gttggacatg acattgcgcc ggtacccggg
      901 ggacaagacg tacagcagat ctccgaggcg gtccgcgatc cagtagcgta ccggcgtcgg
      961 aagcgcgcgc gcgagccgcg acgatgcccg tgcaaccgag acgctgagga aatggcggat
     1021 gtagtgcgga tcctcgcgcc ggaaccgctc gctgaccttg cgggcgagtg tggatcgctt
     1081 ctcaggcacg gcgccctgag attgaagatt gtcccgatcg ccggcacggg ttcgaatggc
     1141 catggttcgc gttcatttcg agagacatgt cagccagtcg ttgactcatg caccgcttca
     1201 ggagccggag tcgccggctt gttccagagc gcagttgcat cataaatcac aggaagccca
     1261 cgaacgaccg tgagggtcac cgaaacgacc acgcacgccc aaccggccca ccggagcgga
     1321 tcccacgagt agatctggtg gatcagggag tccggggcgt cacgatggcg atatccttcg
     1381 agacccgtca ggaagaggaa gccaaacgcc ttggtccacc cgaacaaggt gcgcatgaac
     1441 cgacccgcag tcagccaacg ggtgaactct gaccgcatca tgttccgctc gccgaaagct
     1501 gtcattcctt ctgcgtacga catcgaacga ataccgtcga cgatgaatcc gcgggtcaga
     1561 accacaagcg gaatccagac cggaatcagc tgcagctcgg caaagatgac ccagagcgcg
     1621 ttttcgacga tgcggtcgcc ggcaatgtca aagacggcgc caaactggga ggtggaattc
     1681 gtccggcggg caatgtaacc gtccaggccg tcgccggcaa atacgatggc gatgaccaca
     1741 attgcgacgg caatccatgc cgcgctgccc cagtagatca tcccgatcgc cacgaagagc
     1801 aatatgagcc gggcgatggt gatcaggtta gccacgcaca tctcctacct gcggccatgg
     1861 ggtaccgcaa attcgcgcgc actaccacaa gagattgatt gccccgaccc atatcaggca
     1921 gtgaaccatc aacgcgccta cgaccaggaa ttggacagcg tatgcgtccc gcgtgcgcag
     1981 ccaccaaccc agccccaacg ccataattgt cgacaagagc gcgaagaacg gcagacgcca
     2041 gatcgatccc ttggtgccaa acatcgtggt cgtcccttcg gcgtccacat gcagcgggat
     2101 gtagtccgtg atagagcctg accgaatcag gatcatgatc gacatcagca cgagggatgc
     2161 aagcgcgacg accccgccaa tgacgatcgg ccgcaactcg ggactcgggt tcgcgaagat
     2221 cgcgctcatc tcccgaatgc gctgctgggg aatgccgggc atctctcgcc gcgcgaccgg
     2281 ccggcgagcc cgacctgccg gcgctatggc gtcgatgtct gggtcgtcgt aaccctcatc
     2341 tgcatactcg atgtcgagct cggactgcac gcgacgtggc gaccgattcc cgacgcgccg
     2401 gaggcgatcg tacggatcac gcgaggatcg gtaggatcgc gggtcggcct cttcctcttc
     2461 gtatcgcggc tgactgcgaa agcgatcgga atagcgctcg cgccggggcg gagcggcatc
     2521 gtcctcatat cgggaacggc gccgtggtcg tggttcggac tcgattgcgt caggcgggcg
     2581 ggatgacttg tttgaccggc tgccacgctc atcctgggca acgtcgtcgt agtcgggtcg
     2641 gcgcctgatc gaatcaccag acggctgttc ctgccgagag tcgcgccgcc gcccgtaggg
     2701 atcgcctgac cggctggttc ggtctcgatc ttgatcatcc atagggcctc ccgactctca
     2761 cagcactgtg ccggaacgcg tcacgtgaca gcgtcagaat accaaagcac gcgggcttag
     2821 ctgccttccg gccactttcc aacagtggcc ggaagacgct gatcacggcg ttgcggtggc
     2881 gcttgccatg gcggtcggcg atgcgattgg actcgcgacc gggcttgcgt tcggcgagcc
     2941 gaccggactg gccatcggag aggcgattgg acttgccaca ggcgagccga tgggcaatgc
     3001 ctgttcctct gtcggcgtcc cggattcggt cacaggagat gcctccggcg gcagcggggt
     3061 ctcggtcggc aaaggcggcg catccggcgg cgggacaaag gagctcacag ccggagtcgg
     3121 ggtcggctcg accttggacg agatatccat gccggtcttt tgctcctcga gccaccggga
     3181 gacgatgcta tcccggtact gctggagctg ctgatcggtc atcggccgat tctggtcgac
     3241 ctcatatacc ttgacgatgt gccaaccgta ttgcgtctgg aatggttgac tgatctggcc
     3301 aggctgcagc gagaaggcga catcttcgaa cggcttcacc atctggccgc gcgtgaacca
     3361 gccgagatcg ccgccattct cagccgtcga cgtatcgttg gactgctgcc tggcggtttc
     3421 ctcgaagttg gcgccaccct ggagctccgc gtagatcgag tcggcaaggt ccttggtatc
     3481 gaccaggatg tgggccgcgt ggacctgcgg cgccgtctgt ccgacatctt tcagcagctg
     3541 gccctcgatc ttctctcgag caatagccgg cctgacgatc aggttctcgt aatcgctatc
     3601 cgacatgtgc gcgatgtcga agacagcgct cttgtagttg ccgtagctcg cctcagacgt
     3661 tgcgattgcc tcagtggggt tcggtgtggc cgcctccggc gttgccgact cctgcgtgat
     3721 gggcgttgcc tggttgaccg gtgaaccgac gggagacgct gccggtgacc cgaccagtgt
     3781 tcccggggtg ccttgtactg ccagcggtgt cgctgccgat ccactcgggc ttgcctgagc
     3841 gaccggacta gccgctgtcg acggaatcac aatgacaatc ggcgtaccac tgatcggtga
     3901 ggccgcagga gtaccggcaa ccggggaacc gatgggagtg ccggcaaccg gcgagccgat
     3961 tggggtcgcc tgaccggcaa tcggggttcc gccgcccgcc gcaggtgatc ccacggcgga
     4021 gaccggagtc ggcgtttggt tctcagcctg aagcgcgacc tcggtctgcg tcgcccattc
     4081 ggcacgctgc gggatcagcg tcggcgacgg agtcggcgtg aagatcggcg cgttttgcgg
     4141 ctcaaactgc tgccacaggt agttctggac atcctgatcc gtgaccgaaa ggcccatatc
     4201 atcgaggctc tggagataga cctgatcttc gaccattcgg gtcagcgtgg aatcgttaat
     4261 gtcggtcgag ccccagacct tgtcgagctc cgacgcggcc tgctgggcca gtgtcagata
     4321 ttgctgctgc tggctgggat cgacgagctg ggagaactgg ttgtactggt taacctggtt
     4381 gaccagctcg accgagcgca ccttccagta atcgcgccgg cgaatttcca cgccgttgac
     4441 cgaggcaagg accgcgcgcg gcttgaggaa gtactcattg agggcgaaac cggccaggat
     4501 aacgagcgag aggacgccgg cgatcgccag gccgatatag agttgccgac gttgtcgttc
     4561 ttcgcgctct cgtctggaga ttctgcgctg gctggcaggg ccggccgcgc cacttcgttt
     4621 cttcgggagc gtatcccgcc ggcgttgcga cataaacctc aaactctcca gacggtgatt
     4681 ggcgctcagg cgagatgcgc cgctggatcg atgtcgggca gcgcgcccat tacgatccag
     4741 cggcgagatg ttctagaagg ggaggtcgtc gatatcctcg gctacggcag ccggctcggc
     4801 agaccgaccg ccaccataac cgccctgacc gccgccttca ccgcgggcac tcatgagcag
     4861 gacctcgtct gccgacacat caagcgaaaa acgtgtttca ccctgctggt tggtgtattc
     4921 ccgttgctcg atccgcccac tgacgaaaac tcgctgacct ttggcgagaa ctccctcttg
     4981 ggcaaagcga tcgagccgct cggccaactt cgaccaagcg gtcacccgaa accaggtcgt
     5041 tcgctcctgc tgggtgcccg atgcgtccgt gaaccgattg gaggtcgcaa cagagaaatt
     5101 gacgttcatc gcgccggact tcgtgtatcg agtctcaggg tcttgcccga gattcccgat
     5161 tatctgaact ttcgcgtaca tatcatctcc ctcgatgatc aggacgcccg ttactcgacg
     5221 ggcgcctcgg tgtcagatgc ctgttcagca gtttcggttg ccggctcggc agtttcggtt
     5281 gcctgttcag cagtttcggt tgccgcctcg ccctccgcgg tgctcaccgt cgccgctggc
     5341 tgagcggcct cggtcgcctc accttcagcc gcttcctcag ccacttcgcc ctcggccggg
     5401 gtcggctcca tgagcggagt agtctgctgg gtcagcaaat agcggatgac gcgatcctgg
     5461 agtttgattt cgcgctcaat ctcttcgatc ttgccggatt cggccgtgaa atagtagagc
     5521 acatagacgc cgtcccgaat gtcgcggctg ccgtggcgga tcgggtaggc aaggcggcga
     5581 cggccccaag gcgcctcccg gttgaccagg cgaatcgccc ccgcggcgcc ggtgatgtgc
     5641 gcttcgacct tgccaccctc gctgaccaga tcttcctcgg acagctccgg gtgaaagatc
     5701 accatcaact cgtagtcgcg tgttcgcttg aagtcagtgg acatgaatcg aagaggctcc
     5761 ttcctctgga gtcgtccccg gccgatcaat acggccaaag ggacagaaag gctcaggtat
     5821 tgaacccaca aatcaggcga gcctgccggc tcgccggact gccaattata ccatgatttc
     5881 tagtcagaaa gcgtcagtcc aaggatgccg gcaagctccc tcaggtcgcg ctttgcctgg
     5941 gaaatatccg tgtcttcggc ggacagaatt tcgctcgtca ggagccgcaa ttgctcgatc
     6001 gccgccggcg tatcgaggtc gttctccatg gccgcgatga atcgcttgcg atacctcgac
     6061 gaatccagca tttcgccgat cgagtacgag ggggtgtcga tagcctcgca catgtcgcga
     6121 gccaattcgg cgaacccgtc gatctcttcg tcgatgtact cccatggtgt gcggtagtga
     6181 tgcgaaaaga ggtagaggcg gaatgcatca gccggataat ccgcgagtac gttgctcgcg
     6241 atcaccagat tgccgagcga cttgctcatc ttctcgccct ggtactcgac catggccacg
     6301 tgcatccaat agcgcgtgaa gggcctgaca ccggtcgcgt tctcgctttg ggcgatctcg
     6361 cattcgtgat gcggaaagat caggtcggcg ccgccgccat ggatgtcgac cgtgttgccg
     6421 agcacgctca ttgacatcgc gctgcactca atgtgccaac ccggccggcc cgggccccac
     6481 ggcgaatccc aggtcggctc acctggcttg gccgcctgcc agagcacgaa gtcgatcgga
     6541 tcctgcttgt tcgggtcgtc cgggttatta ccgcgctcat tggcgacggc cagcatctcg
     6601 ccacggggaa tccgcgaaag cttcccgaac tcgggatcgg acgaaaccga aaaatagacg
     6661 ctgccgttgg cgacgtaggc gtgcccctcc tcgaccagcg tctcaatcag tgagaccatc
     6721 aaggggacgg tctcagtggc ccggaggtag tggtcgaacg gcagcgcgtt cagttggcgc
     6781 atgtcgttca ggtacttatc ggtctcactc cggcccagct catcccaggt cgtgccgagc
     6841 tccttcgact tccgcaagat gtcgtcatcg atgtccgtca cgttctgggc gtacgtcacc
     6901 ttgtatccgc gagaacggag gaatcggacg aggatgtcga aggtcagaaa cgtgaaggca
     6961 tggccggcat gcgtggtgtc atacggggtc acgccgcaga cgtatatccc gacgtggttg
     7021 tcgattacgg tcagcggctc gaccgaacgc gtcgctgagt tgtaaaggtt catcagactc
     7081 ctgctcgcca gcctcgcaga ctgtcaattc gattctcgat taccctgttc acacagggct
     7141 caggcttggg cgagagtata ccgatctcaa gagggatttc gtgtcgggtt ttggaagacg
     7201 tctccttcga cgtgtctctg ccgtcaacgc gttcatcgtt gcattgactg tgatgttgct
     7261 cgccgcgtgt ggcggacccg atctcggcaa cccggccgat cacacgccga tcccgagcag
     7321 tactccgaca atcgcccgac caactgccgc cgtcggcagg acgaccggag cgccgtgcga
     7381 gtccggaacc ctacgcatca aggacctgcc ggagattcag aagcgctggc aatcggggct
     7441 ctcggtcgcg cggggtcggg ctgcggcctg gaaggaagac gcggtcctga tcgagctcgg
     7501 catctcgtgc gagttgttcg aatccggttt ccggtggcag gcgacatttt tttcgcggga
     7561 cgcccaggcg tattaccgct ccgacaccac agaggtcatc ccagtcaaca ccgacccggc
     7621 cgcgatcatt ccgctcccgg acagcgagat caactttgac ggcctgtacg ccgtcttgat
     7681 gcaatccgat aaggtcgatg ccaacggtga cgacaccata accgcgctcg atgtccgggt
     7741 cagcaccgag ctgcagccaa tcggcccgcc cggtgttccg accggcgccg cgatctacca
     7801 tgttgcatta cgctcgcatg ggttggtcat cgagatgtat atcgatgccg ttcagggtca
     7861 gatttaccag ttccagtagg ggaggccgtg gaggtagcgg aacgactgct tgagttggcg
     7921 gtcatggggc ggatggccct ctacaccgac atcgacggga cgatctcgcc gatcgcggcg
     7981 acgccgtccg acgcgttcct gttgccgggc gcggccgagg cactcagcat gatctgcgcg
     8041 catgggatcc gggtcgtggc gattagtggc cgcgcggccg ccgatgcgcg ccggctggtt
     8101 ggcttgccgg agttggacta tgccggcaac cacggcttcg agctgatgac ggccaagggg
     8161 catgtgatcg cggaagatgt cgccgcggcg tcgacgagcg taaaggcggc gctcggcgac
     8221 ctgatcgcgt tgagcgacgc gctgcccacg ggtctggtga tcgaggacaa gaccttcacg
     8281 ggctccgtcc attaccggct gacgaagaat cccgacgcgt ctgcaatcgt cctcaaggcg
     8341 gtcctgtccg acatcgcggc tcgaaatgga ttgctgttga ccgaggggcg gctggtgtac
     8401 gagctgcggc cacgcctgga catcaacaag ggagtgttcg ttatgaacga tgtccgcctg
     8461 caaggaatcc ggaccgcggc ctttctcggg gatgacatca cggac
//
LOCUS       DATAOZ010000102         3497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6882233, whole genome shotgun sequence.
ACCESSION   DATAOZ010000102 DATAOZ010000000
VERSION     DATAOZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6882233"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VHR64_02930"
     CDS             <1..295
                     /locus_tag="VHR64_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX3301808.1"
                     /translation="GNAQRLRIMAALVPGRNYVSQLARDLKMSRPLLHLHLQRLEAAG
                     LITGSLELSPDGKAMKFYEVPTFQIELSPDSIVAAAETLTDQGHESDSKEGSR"
     gene            297..569
                     /locus_tag="VHR64_02935"
     CDS             297..569
                     /locus_tag="VHR64_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301809.1"
                     /translation="MGWPEAVVASVSTAFFFAALAVGIVWVMKVGAKKAEAEASSEHT
                     MMVRSLAEEATASQRTVATELTEVRLAMAEIRDRLAAIERMMTEVG"
     gene            741..3011
                     /locus_tag="VHR64_02940"
     CDS             741..3011
                     /locus_tag="VHR64_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3301810.1"
                     /translation="MGQFSTAGMARLTSRHPWRTIIVWVVILAIAGWQASLIGDRTTS
                     DFKFNTKPESVKGFDLIEEHMGGKQALTETVVVSSDTLTVDDPEFQNAVAATTTALRG
                     MPDLVTNAVNYVELSQSADPNAQAQANALVSADRHATLIPVTLAVTELPSASNEEKYI
                     KTVESNGTGSVRVLTVGAISSDHTFTKISEEDLIKGEGIGIAAALVVLLVVFGALVAI
                     GLPLALALISIAIATGLASVIASVSTISFFVSNMISMIGLAVGIDYTLFIVERYREER
                     RHGRPKQEAIEVAGGTASKAVLFSGLTVVFALLGLFMIPTTIFRSLGLGAVLVVVVAV
                     LAMLTLIPVMLSLLGDRIDWPFHRHYDDPEHIAAQQMRDQQTLHGGFWGNITRVVMAR
                     PVISMVLSAGLLIALAIPYFDLTLGFSGISTLPKSNIRDGYEILAKDFQAGRLSPIKV
                     VVEGQQGEVNQNISGFLASITNDPVLGSYEQPTWSSDGEMAVININLPTDPDSQVARD
                     AVTRLRDSVVPQSFNGSSANVYVSGDPAFNYDFRSLISTWTPIVFAFVLLLSFILLTL
                     AFRSIVVPIKAILMNLLGVGAAYGILVLVFEKGYLHNVLGFQQVPAIEPWLPIFLFCV
                     LFGLSMDYHVFLLSRIREHYDQSHRNRESVAIGLQSTARIITGAALIMVSVFFGFAAG
                     RLVMFQQMGFGLGMAVLIDATIIRSILVPSAMALLGDRNWYMPSWLKWLPDLRVEGAP
                     RPAVPAPTPSPQPVGD"
     gene            3095..>3497
                     /locus_tag="VHR64_02945"
     CDS             3095..>3497
                     /locus_tag="VHR64_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3301811.1"
                     /translation="MNFDDSPDGQMITQVSPDSTDVIHRDLNRTWPVIDRGEGIYLYD
                     TEGRRYIDGSGGSAAVTAIGHGVPEVIEAMTSQAKRLAFAPTHAFTTEPIEECARLIV
                     EKFAPAGMDRVFFCSSGSEANENAIKMAIQYH"
BASE COUNT          635 a         1124 c         1080 g          658 t
ORIGIN      
        1 gggcaatgcc caacgcctcc ggatcatggc ggcgctcgtt cccggacgga actacgtcag
       61 ccaattggcg agggatctga agatgagccg cccgcttctc catctgcact tgcagcgcct
      121 ggaggcagcg ggactgatta cagggtcgct ggaactgtca ccggatggca aggcgatgaa
      181 gttctacgag gttccgacat tccagatcga gctgagtccg gattcgatcg tcgcagcggc
      241 agagacactc acggaccagg gtcacgaatc ggattcgaaa gaagggagca ggtagtttgg
      301 gttggccgga agcagttgtt gcgtcagttt cgaccgcgtt ctttttcgcc gcgttggcgg
      361 tcggcattgt ctgggtgatg aaggtcggtg ccaagaaggc cgaggcggag gcctcgagcg
      421 agcacacgat gatggtgcgg agcctggccg aggaggcgac cgcctcccag cgcacggtcg
      481 cgaccgaact caccgaggtg cggctcgcga tggccgagat tcgagaccgg ctggccgcga
      541 tcgagcggat gatgaccgag gtcggttaac gcttcgttaa acccgtttcc agagattgac
      601 attggttttt ctacgctgta gagtcagccc gttggcgatt ctacggtgta gaaaagcgac
      661 cacttcacac gcgtaccaag aggggcagcg acggtgcctc ccgccccagg cgagagcgac
      721 cgttcggagg aaggcaggca atgggtcaat tttcaacagc gggcatggca cgactgacgt
      781 cgcgccatcc ctggcgcacc atcatcgtct gggtcgtgat tctggcgatc gccggatggc
      841 aggcatcgct gatcggagat cggacgacgt ccgatttcaa gttcaacacc aagccggagt
      901 cggtcaaggg tttcgacctg atcgaagagc acatgggcgg caagcaggcg cttactgaga
      961 cggttgtcgt ctcctccgac acgctgacgg ttgacgatcc cgagttccag aacgcggtcg
     1021 cagccacaac caccgcgctc cgcggcatgc ccgatctcgt gacgaacgcg gtcaactacg
     1081 tcgagctcag tcaatccgcc gacccgaacg cgcaggcgca ggccaatgcc ctggtctcgg
     1141 ccgacaggca cgccaccttg atcccggtca cgcttgccgt gaccgagctg ccttccgcgt
     1201 cgaatgagga gaaatacatc aagacggtcg aatcgaacgg cacggggtcc gtccgcgtcc
     1261 tgaccgtcgg cgccatcagc tcggaccaca ccttcaccaa gatctcggaa gaagacctca
     1321 tcaagggcga aggtatcggc attgccgcgg ccctggtggt gctgctggtc gtctttggcg
     1381 cgctggtggc gatcggactg cccctggcac tcgccctgat ctcgatcgcg atcgcgaccg
     1441 gcctggccag cgtgatcgca agcgtcagca cgatctcatt cttcgtcagc aacatgatct
     1501 ctatgatcgg tctggcggtc ggtatcgact acacgctctt catcgtcgag cggtaccgcg
     1561 aggagcgccg gcacggtcga ccgaagcagg aagcgatcga ggtcgccggc ggcaccgcca
     1621 gcaaggccgt cctgttctcc gggttgaccg tcgtcttcgc gctgctcggc ctgttcatga
     1681 tcccgacgac gatcttccgc agcctcggcc tgggcgcggt gctggtcgtg gtcgtcgcag
     1741 tcctggcgat gctgaccctg atcccggtga tgctctcgct cctgggcgat cgcatcgact
     1801 ggccgttcca tcgccattac gacgaccccg agcacattgc cgcccagcag atgcgcgatc
     1861 agcaaacgct gcacggcggc ttctggggca acatcacccg cgtcgtgatg gcccggccgg
     1921 tgatctcgat ggtgctctcg gctggcctcc tgatcgcgct ggccatcccc tacttcgatt
     1981 tgacactcgg tttcagcggc atcagcacct tgcccaagag caacatccgt gacgggtacg
     2041 aaatcctggc caaggacttc caggccggtc gcctcagccc gatcaaggtg gtcgtcgaag
     2101 ggcagcaagg cgaggtgaac cagaacatca gcggattcct ggcctcgatc acgaacgatc
     2161 cggtgcttgg cagctacgag caaccgacct ggagctcgga cggcgaaatg gccgtgatca
     2221 acatcaatct gccgactgat cccgactcgc aggtcgcgcg ggacgcggtc accaggctgc
     2281 gcgactcggt cgtgccgcag tcattcaatg ggtcatcggc gaatgtctat gtgtcgggcg
     2341 atcccgcctt caactacgac ttccgatcat tgatcagcac ctggacaccg atcgtcttcg
     2401 ccttcgtgtt gctgctcagc ttcatcctgc tgacgctcgc attccgctcc atcgtcgtgc
     2461 cgatcaaggc gatcctgatg aacctgctcg gcgtcggcgc ggcctacggc atccttgtgc
     2521 tggtcttcga gaaggggtac ctgcacaacg tgctcggctt ccaacaggtg ccggccatcg
     2581 agccgtggct gccgatcttc ctcttctgtg tcctgttcgg tctcagcatg gattaccacg
     2641 tcttcctgct gagccggatc cgcgagcact acgaccagtc gcaccgcaat cgggagtcgg
     2701 tcgcgatcgg cctgcagtcg accgcgcgga tcatcaccgg cgccgccttg attatggtct
     2761 cggtcttctt cggcttcgcg gccggccggt tggtcatgtt ccagcagatg ggcttcggcc
     2821 tcggcatggc ggtcctgatc gacgcgacca tcatccgctc gatcctcgtt ccttcggcta
     2881 tggcgctgct gggagaccgc aactggtaca tgccgagctg gctgaagtgg ctgccggacc
     2941 tccgcgtcga gggcgcaccc cggcccgccg tgccggcgcc cacgccgagc ccacaaccgg
     3001 tcggcgacta gcgacagtcc acatctcctt cttccggacg ggcggcgagc gcaagctccc
     3061 cgcccgtctt cttttcgggc gtagaatcca cagcatgaat ttcgacgact cgccggacgg
     3121 gcagatgatt acgcaggttt caccagacag caccgacgtc attcaccgcg acctcaaccg
     3181 aacctggccg gtcatcgacc gcggcgaggg catctacctc tacgacaccg aaggcaggcg
     3241 ctacatcgac ggctccggcg gctccgcagc cgtgaccgcg atcggacacg gcgtgcctga
     3301 ggtgatcgag gcgatgacca gccaggcgaa gcggctcgcc ttcgcgccga cgcacgcatt
     3361 cacaaccgag ccgatcgagg agtgcgcgcg actgatcgtc gagaagttcg ccccggcggg
     3421 catggaccgt gtcttcttct gctcgagcgg gtcggaggca aatgagaacg ccatcaagat
     3481 ggcgatccag tatcacc
//
LOCUS       DATAOZ010000103         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1358409, whole genome shotgun sequence.
ACCESSION   DATAOZ010000103 DATAOZ010000000
VERSION     DATAOZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358409"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="ftsZ"
                     /locus_tag="VHR64_02950"
     CDS             complement(<1..1040)
                     /gene="ftsZ"
                     /locus_tag="VHR64_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX3301812.1"
                     /translation="MQQPLHGEFSNPFARIKVIGVGGAGGNAINRMVDAGVDGIEFVA
                     VNTDGQALLNSRAPLAVRIGDKLTKGLGAGGRPEVGLRAAEESSDSIGEIARDADMIF
                     IAAGMGGGTGTGASPVIARIAKSAGALTVGVVTRPFDFEGNRRKRSADEGISQLRDNV
                     DALITIPNERLLTMVDPKTSISEAFQIADEVLHQGIAGLSDLITKPGMINLDFADVRT
                     IMRDAGSALMAIGYGEGENRTIDAAKQALESPLLETSIQGATGGLYNISGSRNLTLLE
                     TSEAAEIIRAAVDEDAEIIYGTSIDESMGDAVKITLIATGFDDHESYQPYAAPRRARP
                     ARQQEPVYQLNPR"
     gene            complement(1306..>2437)
                     /locus_tag="VHR64_02955"
     CDS             complement(1306..>2437)
                     /locus_tag="VHR64_02955"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX3301813.1"
                     /translation="RAPPRNRVHRRHGRDGMTNKPKVNVLVLFGGQSGEHDVSLRSAQ
                     TVIGALDSERYAVRQVGITREGGWIASGDPMAALTASSPLFQLGDGKAPVGEIHAEPG
                     LPVLARDTVDVVFPVLHGPMGEDGTIQGMLELAGIPYVGSGVLGSAVAMDKAMAKLVL
                     SQAGIPQAKWALVNRSEWATDQESVVASIVADLGLPCFVKPANMGSSVGVSKAHDEAE
                     LRQAINFAHEYDRRVVIEEAIDARELEISVLGNDQPIASVAGEIVPCNEFYDYNAKYV
                     DDDSELIIPADLSPDKLAEMQEIAIRAFRALDLAGMARVDFFLERDTERLLLNEVNTI
                     PGFTSISMYPMLWEASGLPLPELVDRLIDLAVERHSHPRRVV"
BASE COUNT          417 a          812 c          744 g          464 t
ORIGIN      
        1 cgtggattga gctggtagac cggctcctgc tgtcgggccg ggcgcgctcg ccgcggggcc
       61 gcatacggct ggtacgactc gtggtcgtcg aagccggtcg cgatcagcgt gatcttgacg
      121 gcatcgccca tcgattcgtc aatcgaggtg ccgtagatga tctcggcatc ttcatcgacc
      181 gccgcgcgga tgatctcggc cgcctcactg gtctcgagca gcgtcaagtt ccggctaccg
      241 gagatgttgt agagaccgcc ggtggcgccc tggatgctcg tctcaagcag cggactctcg
      301 agtgcctgct tggccgcgtc gatggtccgg ttctcgccct cgccataccc gatcgccatc
      361 agggcggagc cggcatcgcg catgatcgtg cggacgtcgg caaagtcgag gttgatcatg
      421 cccggcttgg tgatcaggtc ggaaagaccg gcaataccct ggtggagcac ttcgtcggcg
      481 atctgaaacg cttcagagat cgaggtcttg ggatccacca tcgtcagcag gcgctcattc
      541 gggatcgtga tcagggcatc gacgttgtcg cggagttgac tgatgccctc gtccgccgat
      601 cgcttgcgcc ggttgccctc aaaatcgaat ggccgtgtta caacgccgac ggtcaatgca
      661 ccggcagact tcgcgatccg ggcaatcacc ggcgaggcgc cggtcccggt gccgccgccc
      721 atgccggccg caatgaagat catatcggca tcgcgcgcta tctcgccgat tgagtcggag
      781 ctctcttcgg cggcgcgcaa accgacttcc ggtcgaccgc cggcaccgag tcccttggtc
      841 agcttgtcgc cgatccggac ggccagcggg gcgcggctgt tgagcagggc ctggccatcg
      901 gtattgacgg cgacgaactc gataccgtcg acaccggcat cgaccatgcg gttgatcgcg
      961 ttgccacccg cgccgccgac gccgatcacc ttgatccggg caaatggatt cgaaaactcc
     1021 ccgtggagtg gttgctgcac cgtatgctcc tcagcccgac gtaccgtgcg ccctgttgtt
     1081 cgccgtccaa taaattgacc agccgtgggt cgcccctgat cggccggtcg tcctggttct
     1141 gatcgcggat ctcgaggttg cattctgccc cattcggcgt ttcccgctca cggcgtgcag
     1201 gccagtgtgc gcgtaatcag gaatctgcgc accttgccct gcatgccgat gcgaccgaga
     1261 ccgcattata acagttagcg tggagattcc cctagatttc catcgtcaga cgacccgccg
     1321 cggatggctg tgccgctcca ctgccaggtc gatcaatcgg tcgaccagct cgggcagcgg
     1381 cagtccgctc gcctcccaga gcatggggta catgctgatc gaggtaaagc ccggaatcgt
     1441 gttgacttcg ttgaggagca accgctccgt atcccgctcg aggaagaaat cgacccgcgc
     1501 catgccggcc agatcgagcg cccggaacgc ccgtatcgca atctcctgca tctcggccag
     1561 cttgtccggc gagagatcgg ccgggatgat cagctcggaa tcgtcatcaa catacttggc
     1621 gttgtagtcg tagaactcgt tgcagggcac gatctcgccc gcgaccgagg caatcggctg
     1681 gtcgttgcca agcacagata tctcgagctc gcgcgcatcg atcgcctctt cgatgacgac
     1741 ccggcgatcg tactcgtggg cgaaattgat tgcctggcgc aactcggctt catcgtgcgc
     1801 cttggacacg ccgacgctcg agcccatatt ggccggcttc acgaagcagg gcagtccgag
     1861 atccgcgacg atcgatgcga cgacgctctc ctgatcggtg gcccattcgg accggttaac
     1921 cagcgcccac ttcgcctgcg gaatgccagc ctggctcagg accagcttgg ccatcgcctt
     1981 gtccatcgca accgccgagc cgagcacgcc ggacccgaca tacgggattc ccgccagctc
     2041 cagcatgccc tggatcgtgc cgtcctcgcc catcggccca tgcagcacgg ggaagacgac
     2101 atcgacggtg tcgcgcgcaa gcaccggcaa ccccggctcg gcgtggatct cgcctaccgg
     2161 cgccttgccg tcgccgagct ggaagagcgg tgatgaggcg gtcagggccg ccatcgggtc
     2221 gcccgaggcg atccagcctc cctcgcgagt gatcccaacc tggcgcacgg cgtacctctc
     2281 cgaatccagg gctccgatca ccgtctgggc ggaccgcagg gagacatcat gctcgcccga
     2341 ctgcccgcca aagagcacca gtacgttgac tttcggctta ttcgtcattc catccctgcc
     2401 atgtcgccga tgtactcgat ttcgcggtgg agctcga
//
LOCUS       DATAOZ010000104         4433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5567991, whole genome shotgun sequence.
ACCESSION   DATAOZ010000104 DATAOZ010000000
VERSION     DATAOZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5567991"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VHR64_02960"
     CDS             complement(<1..763)
                     /locus_tag="VHR64_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3301814.1"
                     /translation="MADKFLIIGALGFIGAWTAKRLIDEGAGVVSYDLPGDPHRLKLI
                     MPNSALDKITFVEGDVTDQAGLEKAVRDNDITHIVHLAALQVPSVKANPVLGMKINVV
                     GTTIVFEVAKNFADQVQSVVYASSVAVYGPASNYPPGPIAHDAPLLPTTLYGVTKEAN
                     EGTAEVYWQDYKIRSVGVRPAFVYGPGRDVGMTSTPTKAMLAAAAGRPYHVSFGGTGF
                     YQHGDDVAKALIKSARAKIDGAPCFNLGGTRVPMTD"
     gene            complement(797..1627)
                     /locus_tag="VHR64_02965"
     CDS             complement(797..1627)
                     /locus_tag="VHR64_02965"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX3301815.1"
                     /translation="MAQKQDAMDALLAGFRGSRVVDLTVALSEELPCAWPTHMPFQRK
                     LYNYFADNPNQNQPTWGFRGPYHTAVLTIDEHCGTHIDAPAHFIPPPGSGLPNAAEIG
                     SQTLEKLDLGKMMGPAAVIDVTELTGQGEGGVSPEITPEMIERWEAEHGQLQPEDIVL
                     FRSDWDQHFVPKPEGDNYVLNPFLLQKGPGWPTPGIPALKLLLDRGVTTVGLDGVSVG
                     ASHDGVPPHVFGLGRGMMYVELLANLKEVPPRGAFFIFLPIKIKGGSAGPGRAVALVP
                     "
     gene            complement(1811..2845)
                     /locus_tag="VHR64_02970"
     CDS             complement(1811..2845)
                     /locus_tag="VHR64_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301816.1"
                     /translation="MSADAITVPGGLPGPRTGNPVLRWSARNARIVAPLITLIVEIIF
                     FSFASDVFLTRSNFQNVVSQVGPVAVAATGITFVLLCAEIDLSIASISVFCGIVATYI
                     FSNDWHGLGQWGIVIAAVAGAAIGLINGFFICYVGIPSFMMTLAMLTIAKGMGTYITQ
                     GKPIFDAPPLMEHLASVGNKFLGIPLIGFVALGFLIIGDVILNYTKFGRYVFMTGGNR
                     EAAEMSGVDTRKVLLNVMVISGFTAGMAGMLFVGRLKSANPAAGGNLLIDTIAAVVLG
                     GTSLFGGEGGMKNTALGLLIFAFLSNGLNLLPWVDIYFKEALQGIILVAALVLNVVAL
                     RLEKVQIQTE"
     gene            complement(2906..>4433)
                     /locus_tag="VHR64_02975"
     CDS             complement(2906..>4433)
                     /locus_tag="VHR64_02975"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3301817.1"
                     /translation="EESPSTGSVPPDDSVVLELRNVIKRFGGAVALSGVDFTLRRGEI
                     HGLLGENGAGKSTLMKILSGVHSPDEGEIILEGQPVKFHSPAAAKALGIGMIYQELTT
                     MPTLTVAENVFLGRQYTNRFGLIDWGRMRSDAADALHRLGIDIDVTQRMGRLSLGNQQ
                     LVEIARIILSGAKIIVLDEPTSALSGPESERLFSLMRQLKARGTSMIFISHFLEDVMA
                     VSDRVTILKNSRKVAELENVGLTKAKLVELMIGRDATELAESYEGGTQLPPRVDGETA
                     LKVESLSVPGEFEDITFDVKHGEILGMFGYLGGGMTEVARSLFGRLKPSQGTIVLDGE
                     PVRPTTPIKAKRLGIAYLTENRRATIFPRHAIYKNITLAHLTHLVPALFSARSEVTVA
                     DEQIRLTGVRPANSRMLAGHLSGGNQQKVVLAKWLTRRPKVLILNEPTRGMDVGAKRE
                     VLDLIRKLKAQGVAIILMSTEPETVMAESDRIIVMSKGKITAEFVDQEVTKEQLLVHA
                     "
BASE COUNT          849 a         1404 c         1379 g          801 t
ORIGIN      
        1 catcggtcat cgggacccgc gtgccgccga ggttgaagca gggggcgcca tcgatcttcg
       61 cccgcgccga cttgatcagc gccttggcta cgtcgtcgcc gtgctggtag aagccggtcc
      121 ccccgaagga gacgtgatac ggacgaccgg ctgccgcagc cagcatcgcc ttggtgggcg
      181 tcgaggtcat gccgacatcc cggcccggac cataaacgaa ggccggccga acgccgacgc
      241 tgcgaatctt gtagtcctgc cagtagacct cggccgtccc ctcgttggcc tccttggtca
      301 cgccgtagag cgtggttggc agcaggggag cgtcgtgggc aatcggacca ggcggatagt
      361 tcgatgccgg gccgtacacc gcgaccgagc tggcgtaaac gaccgactgg acctgatccg
      421 cgaagttctt cgcaacctcg aagacgatcg tggtgccgac cacattgatc ttcattccga
      481 gtactgggtt tgccttgacg ctcggcacct ggagcgcggc aaggtggacg atgtgcgtga
      541 tgtcgttgtc ccggaccgcc ttctcgagtc cggcctggtc ggtcacgtcg ccctcgacga
      601 aggtgatctt gtcgagggcg ctgttcggca tgatcagctt caggcgatgc ggatcgcccg
      661 gcagatcgta cgagacgacc cccgcgccct catcgatgag tcgtttggcc gtccacgcgc
      721 cgatgaagcc gagcgcgcca atgatcaaga acttgtctgc caccgagtcc tgctccctct
      781 gtatgaatga attggcttac ggaacgagcg cgacggcccg gcccggaccg gcgctgccac
      841 ccttgatttt gatcggcaga aagatgaaga aggcgccgcg cggtgggacc tccttcagat
      901 ttgccagcaa ctcgacgtac atcatgccgc gaccgagacc gaagacgtgc ggcggtacgc
      961 cgtcgtgcga cgcgccgaca ctaaccccgt cgagaccaac cgtggtcacg ccgcgatcga
     1021 gcaggagctt cagtgcggga atacccggag tcggccaacc cggccccttc tggagcagga
     1081 acgggttgag gacgtagttg tcgccctctg gcttcgggac aaagtgctga tcccagtcgg
     1141 agcggaagag gacgatatcc tccggctgaa gctggccatg ctcagcctcc cagcgctcga
     1201 tcatctcggg cgtaatctcg ggactgaccc caccctcgcc ctggccggtc aactcggtga
     1261 catcgatcac cgccgcggga cccatcatct tgcccaggtc aagcttctcg agcgtctgcg
     1321 agccgatctc ggccgcattc ggcaggcccg atcccggagg agggatgaaa tgggccggcg
     1381 catcgatgtg ggtaccgcag tgctcgtcga tggtcagaac cgccgtgtga tacgggccgc
     1441 gaaaccccca cgtcggctga ttttgattcg ggttatcggc gaaatagttg tagagcttgc
     1501 gctggaaggg catatgtgtc ggccaggcgc atggcaattc ctctgacagc gcgacggtca
     1561 gatcgaccac cctgctgccc cggaacccgg ccagcaacgc atccattgca tcctgtttct
     1621 gggccatctc tctcgacctc ggactacgac ggatcaactc cgacattttc gcatggcaag
     1681 gcgagcagtc gggaggcgag caggcgagca gatggtagat ggacgttgga cgatggtaga
     1741 tggaaaagac taacggccat ttaccaacga ccgtctacca acgtccaacg actatccacc
     1801 gtcaacccgg ttactccgtt tggatttgca ctttctccag gcggagggcg acgacgttga
     1861 ggacgagggc agcgacgagg atgatgccct ggagcgcctc cttgaagtag atgtcgaccc
     1921 agggcagcag gttgaggccg ttgctgagga aggcgaagat cagcaggccg agcgcggtgt
     1981 tcttcatgcc gccctctcca ccgaagaggg acgtcccgcc gagcaccacg gccgcgatcg
     2041 tgtcgatcaa caaattgccg cccgccgccg ggttcgcgct cttgagccgg ccgacgaaga
     2101 gcatccctgc cattccggcc gtgaaaccgg agatgaccat cacgttcagc agcaccttcc
     2161 gggtgtcgac gcccgacatc tcggccgctt cccggttgcc gccggtcatg aagacgtacc
     2221 ggccgaattt cgtgtagttg aggataacgt ccccgatgat caggaagccg agcgcaacga
     2281 agccgatcag cgggatgccg aggaacttgt tgccaacgct ggccagatgc tccatcagcg
     2341 gcggcgcatc gaagatcggc ttgccctgcg tgatgtaggt acccatgccc ttggcgatgg
     2401 tcagcatcgc gagcgtcatc atgaatgagg gaatgccgac gtagcagatg aagaagccgt
     2461 tgatcaggcc gattgccgca ccggccaccg ccgcgatcac gattccccac tggcccagcc
     2521 catgccagtc gttgctgaag atataggtcg cgacgatccc gcagaagacg gaaatcgagg
     2581 caatactcag gtcgatctct gcgcagagca gcacgaaggt gatccccgtc gccgcaacgg
     2641 ccaccggacc gacttgcgaa acgacgttct ggaagttgct ccgggtcagg aagacatccg
     2701 acgcgaacga gaagaagatg atctccacga tcagggtgat caggggagcc acgatccggg
     2761 cgttgcgggc actccagcgg aggaccgggt ttccggtccg tggtcccggc aaaccgccgg
     2821 gaacggtgat cgcgtccgca ctcatcgaaa tcctccccaa cccatccctg tcgaacgatc
     2881 cggcgcctgc acgcgcgccg gccacctacg cgtggaccag aagctgctcc ttggtcactt
     2941 cctgatcgac gaactcggcc gtgatcttgc ccttggacat gacgatgatg cgatcggatt
     3001 cggccatgac cgtctccggc tccgtcgaca tcaggatgat cgctactccc tgcgccttga
     3061 gtttgcggat cagatcgagc acctcgcgct tggcgccgac atccatgccc cgcgtcggct
     3121 cattcaggat cagcaccttc ggccgcctgg tcagccactt ggccagcacg accttctgct
     3181 ggttcccgcc cgagagatgc ccggccagca tgcgcgaatt ggccggccgg accccggtga
     3241 ggcgaatctg ctcatcagca acagtcacct cgctgcgagc cgagaagagc gccggcacga
     3301 gatgtgtcaa gtgcgcgagc gtgatgttct tgtagatcgc gtgacgcgga aatatcgtgg
     3361 cgcggcgatt ctcggtcaag taggcgatgc cgagacgctt tgccttgatc ggcgtcgtcg
     3421 ggcgcaccgg ttcgccgtcc agcacgatcg tgccctgcga tggcttgagc cgaccgaaca
     3481 gcgaccgagc gacctcagtc atgccaccgc cgaggtagcc gaacatgccg agtatctcgc
     3541 cgtgcttgac gtcaaaggtg atgtcttcga attcgcccgg caccgagagc gattcgacct
     3601 tgagcgctgt ctcgccatcc acccgcggcg ggagctgcgt gccgccctcg tagctctcgg
     3661 ccagctcagt cgcgtctcgg ccgatcatca gctcgacgag cttcgccttg gtcaggccga
     3721 cgttctcgag ctccgcgacc ttgcgcgagt tcttgaggat cgtgacgcgg tccgagacgg
     3781 ccattacgtc ttcgaggaaa tggctgatga agatcatgct cgtgccgcgc gccttgagct
     3841 gccgcatcag cgaaaagaga cgctccgatt cgggaccgga gagcgcggac gtcggctcat
     3901 cgagcacgat gatcttggcc cccgagagga tgatccgggc gatctcgacc aattgctggt
     3961 tgccaagcga gagccggccc atgcgctggg tgacatcgat atcgatgccc agcctgtgca
     4021 aggcgtcagc cgcatccgag cgcatgcggc cccaatcgat aaggccaaat cggttggtgt
     4081 actgccggcc gaggaaaaca ttctcggcga cggtcagcgt gggcatggtc gtcagttcct
     4141 ggtaaatcat cccgatgcca agcgccttcg cggcggccgg agaatggaac ttgacgggtt
     4201 gcccttcgag aatgatttct ccctcgtccg gagaatggac gccgctcagg atcttcatga
     4261 gcgtgctctt gccggcgccg ttttcgccca gcaagccgtg gatctcaccg cgccgcaagg
     4321 tgaaatcgac accgctcagc gcgaccgcac cgccgaatcg cttgatcacg ttgcgcagct
     4381 cgaggacgac agaatcgtca ggggggacgg aaccggtcga gggcgattcc tcg
//
LOCUS       DATAOZ010000105         5480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4778228, whole genome shotgun sequence.
ACCESSION   DATAOZ010000105 DATAOZ010000000
VERSION     DATAOZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4778228"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1066
                     /locus_tag="VHR64_02980"
     CDS             278..1066
                     /locus_tag="VHR64_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX3301818.1"
                     /translation="MADRASADTSEVRWQKLRRDHIAKLAAYDPVVLQPIGAIEQHGP
                     HLPVDTDTNSVTEVAERAARSLGNRSALVLPTISWGLSPYWMPFAGTITLRPETILAL
                     FSDIGRSVAAHGFRRLIIVNGHGGNGGIIGVAATQMADHGLRAIAMSYWSLIGSELGG
                     ITPGDHGHIGHAGQTETSIQLYLQPDRVDRFYQSVTGWTDLGSMAADLLTAGAYKPPL
                     PLVEAPIGVYGDVTQARAELGQAIIERVSERLSEVVRTFPKSNP"
     gene            complement(1063..1434)
                     /locus_tag="VHR64_02985"
     CDS             complement(1063..1434)
                     /locus_tag="VHR64_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3301819.1"
                     /translation="MNYLVSAEELPSFNVGRPGILAAIFNGGDHCLPGISVILNETLP
                     GDGAPLHRHTYDELFLVQEGRCSFTVAEQTVEAGAGQLVLIPSGAPHGFTNVGDGNMR
                     LVAIHAAPRVEIEWLDAGVPV"
     gene            1696..2178
                     /locus_tag="VHR64_02990"
     CDS             1696..2178
                     /locus_tag="VHR64_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301820.1"
                     /translation="MRALGFSRLLAVAIIAITALSVIGSASPVGAQTARGSIMIHSRA
                     CPLNLPAGSMLFEACHPHPGPTGAQFTVDSRVPKNIGANGNVSFGRVTAGDHLITLTS
                     DFQPNEFLKVRAFCSNSVGGSGIHEATILHGDQAQFWVRVGAGSQLTCDVYFIPESGR
                     "
     gene            complement(2262..2681)
                     /locus_tag="VHR64_02995"
     CDS             complement(2262..2681)
                     /locus_tag="VHR64_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX3301821.1"
                     /translation="MSIPATTRRATYGFGTTLDSPIDEAVERTTAALKAEGFGVLTRI
                     DVDKTLKAKIGVDVEPYVILGACNPGFAHRALQAEPELGLLLPCNVIVYKVGDKSAVS
                     IVDPAQMLGVVDDNPELDALASEVEISLRRVVAALES"
     gene            2813..3763
                     /locus_tag="VHR64_03000"
     CDS             2813..3763
                     /locus_tag="VHR64_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3301822.1"
                     /translation="MPLNSSRPLKVGLLLPETEGQLNGQTAAWSDLLAMTQAAEAIGF
                     DSIWVTDHLIQRSDDGERGMWECWSLISAIAAVTSRVEIGTLVLSTSFRNPAYLAKMA
                     DTVEEISGARLILGLGAGWNEPEYRAFGYPFDHRVDRFEEAMTIITGLLRDGRLDFRG
                     SYYEVADCVLRPRGPRPQGPPIMVGTSGSGPRMLRLAARFADQWNLWFKTFNNRLEDL
                     IQLQAGVDDACRQIGRDPSTLARTAAIKVEVGPHGPSTMSTDPITGSPEEIADTLRQY
                     AAAGISHVQVWPEPNTLDGIAAFEEIFAALDHAPVNPTRG"
     gene            complement(3760..5283)
                     /locus_tag="VHR64_03005"
     CDS             complement(3760..5283)
                     /locus_tag="VHR64_03005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX3301823.1"
                     /translation="MWRTIIAILLASATLLAVPTTHAQTPVASDWQTTTPESQGLDSA
                     ALAKGLLAIRDSGMPIHSLLIARNDKILLDAYFYPYDGADPHDLASVTKSVTTTLVGI
                     AIDQNLLSLDDPVLSFFPDRTIANRDERKEKMTVGDLASMTSGLKCIAEPNEQTLAEM
                     EVSLDYVQFALDLPMSSDPGTEYTYCSPNMHLLSAVLTKASGMSELDFAKKYLFGPLG
                     ITNVVWPADSQGFTHGWGDLYLQPRDAAKIGQLWLNQGDWNGTRVVSKAWVADASSVH
                     AKRPADEDDDYGYGWYIARESDVGGEISAQGRGGQQIQIFPSLGAEIVITAAGIDPGQ
                     ATDLITPALVDPTKTLPANPAGEKELSEAVRVVAQPREPQPVKPLPPVAAKISGVEFC
                     FAPNPLDLASLLLTFNETSVAQIQLTFSGDQPPVGGEVGLDGNYRLAPGRYEFPFGLR
                     GQWVDQQTFALAYDQIANLDAYDARVTFSMDGQSVSMEIKERTEAGSVTLEGRVCTG"
BASE COUNT         1019 a         1708 c         1684 g         1069 t
ORIGIN      
        1 cggcgcgcac ctctgccccg gtcatgtagc ggtgaccatg ggacggacgg gattgcgcgt
       61 cggggctcat ctcgggtaat ccttcggtta ggccgatctc ggccggtgcg gacatacgca
      121 aaacgcggct ggttcatccg ccgcgtcgcg aatatcaagt atagataggc gagcagacgt
      181 gaggcgagca ggcgagaagt cggggacaat ggtcgtcgca gttgatggtt gaacgggtgg
      241 cgcgccaaca cgcgcacgca ggctgagagg agaggttgtg gcggatcgcg cgtcagcgga
      301 caccagtgaa gtacgttggc aaaaactccg gcgagatcac attgccaagc tggcagcgta
      361 cgatcccgtc gtcctgcagc cgatcggcgc gatcgagcag cacggcccgc atttgcccgt
      421 cgacaccgac accaattcgg tgaccgaggt cgcggagcgg gcggccagat cgctggggaa
      481 tcgatcggcg ctcgtgctcc cgaccatctc gtggggactc tcgccctact ggatgccgtt
      541 tgccgggacg atcaccctgc gtccggagac gattttggcc ctgttctcgg acatcgggcg
      601 gagtgtggct gcgcacggct tccggcggtt gatcatcgtc aacggtcacg gcggcaacgg
      661 tggcatcatc ggcgtcgccg caacccagat ggcggaccac ggcctgcgcg cgatcgccat
      721 gtcctactgg tcgcttatcg gatcggagct cggcgggatc acgccgggcg accacgggca
      781 cattggtcac gccggccaaa ccgagacgtc gatccagctt tacctgcaac cagaccgggt
      841 cgaccgcttc taccagtcgg tgaccggctg gactgatctt ggctcgatgg ccgccgacct
      901 cctgaccgcc ggggcataca agccaccgct gccgctcgtc gaagccccga tcggcgtcta
      961 cggcgacgtt acgcaggccc gcgccgaact tggccaagcg attatcgagc gggtttccga
     1021 gcggctgtct gaggtggtgc gcacatttcc gaagtcaaac ccctagaccg ggactccggc
     1081 gtcaagccac tcgatctcaa cccgaggcgc ggcgtggatc gccaccagtc gcatgttccc
     1141 gtcgccgacg ttcgtgaaac cgtgcggtgc gccggacggg atgagcacaa gctgccctgc
     1201 tccggcctct accgtctgct cggcaaccgt gaacgagcag cggccctcct ggaccaggaa
     1261 tagctcatcg tacgtgtggc gatgaagcgg agcaccgtca cccggaagtg tttcattgag
     1321 gatgaccgaa atgccgggca agcagtgatc gccgccgttg aagatggcgg ccaatattcc
     1381 cggccggccc acgttgaatg acggtaactc ttccgctgaa acgaggtagt tcattcggtc
     1441 tcctcccggg caaatcggcg ctattcgtgc gaagtgcgtg tcgagtgtat tcgcgccgtg
     1501 gcaattcgca aatgcctatc gagtcgagca cacggctgtc cgggaacctg ggatatctct
     1561 gcgaaacgat gcgtgaaacc tttgtcgctc atgcgacgtt atgaggctga acatccgaat
     1621 tggctggatg tcggatgcgg cgcttccatg gggaagcgcg gtgtttggtg tcatctgccg
     1681 aagaggagtt ctgccatgcg tgcgttgggt ttttcccgct tgcttgccgt cgcgatcatc
     1741 gcgatcacgg cgctgtcggt catcggatcg gcctcgccgg tcggcgccca gacggcacgt
     1801 ggatcgatca tgatccactc ccgtgcctgt ccgctcaatc tcccggcggg atccatgctc
     1861 ttcgaggcat gtcacccgca tcccgggccg accggcgccc agttcaccgt cgatagccgc
     1921 gttccgaaga acatcggcgc aaatggcaat gtcagcttcg gccgcgtcac tgccggcgat
     1981 cacctgatca ccctgacgtc tgacttccag ccgaacgagt tcctgaaggt gcgagcgttc
     2041 tgctccaatt cggtcggtgg atcgggcatt cacgaggcga caatcctgca cggcgaccag
     2101 gctcagttct gggttcgcgt cggcgccggg tcgcagctga cctgcgacgt ctacttcatc
     2161 ccggagagcg gccgctaact caaggtgccg caccggacaa caagccgcgg ggcggagatc
     2221 actccgcccc gtttttcatg tcccgatgtc cgcgtcgagg ttcaggattc gagcgccgcg
     2281 actaccctgc ggagactgat ctcgacctcg cttgccagcg cgtcgagctc tggattgtcg
     2341 tcgaccacgc ccagcatctg ggccggatcg acgatcgaga cggccgactt gtcacccacc
     2401 ttgtacacga tcacgttgca ggggagcagc agccccaact cgggttcggc ctggagtgcc
     2461 cggtgcgcaa aacccggatt gcaggcgccg aggatgacat agggctcgac atcgacgccg
     2521 attttcgcct tgagcgtctt gtcgacatcg atcctggtca gaacgccaaa tccttcggcc
     2581 ttcaatgccg cggtagttcg ctcgaccgct tcgtcgatcg gcgaatccaa cgtggtgcca
     2641 aatccatagg tcgcgcgtcg ggttgttgct ggaatgctca ttgggatctc cctttcgacg
     2701 catgtacgag gaagcgtcgc gacaccctca ttctatggat ttggcggcgg tatgatgacg
     2761 tattagttgc gcagccatgc ccgtcattcg cacgacaccg gagacaggtt cgatgccgtt
     2821 gaattcttcg cgcccgctca aggttggcct gctgcttcca gagacagagg gccaactcaa
     2881 cgggcaaacc gcagcctggt cggacttact ggcaatgacg caggcagccg aggcgattgg
     2941 tttcgattcg atctgggtca cggatcatct gatccagcga tccgacgatg gcgagcgcgg
     3001 catgtgggag tgctggtcgc tcatttccgc aattgcggcc gtgaccagca gggtcgagat
     3061 cggcaccctt gttctcagca ccagttttcg caatcccgcc tacctggcga aaatggcgga
     3121 cacggtcgaa gagatcagcg gcgctcggtt gatcctgggc ctcggcgccg gatggaatga
     3181 gccggaatat cgcgcctttg ggtacccatt cgaccatcgc gtcgaccgct tcgaggaggc
     3241 tatgacgatc atcaccggcc tcctgcgtga cggtcggctc gatttccgcg ggtcgtatta
     3301 cgaagtcgcc gattgcgtgc tccggccacg cggtccgcgc ccccaaggtc cgccgatcat
     3361 ggtcgggacc agcggctccg gaccccgaat gcttcgcctc gccgcacgct tcgccgatca
     3421 atggaacctc tggttcaaga ccttcaacaa ccgcctcgag gatttgatcc agttgcaggc
     3481 cggtgtcgac gacgcctgcc ggcagatcgg tcgtgatcca tcgaccctgg cccggaccgc
     3541 agcgatcaag gtcgaagtcg ggccgcacgg tccgtcgacc atgtccacgg atccgatcac
     3601 gggcagccct gaggagatcg ccgacacgct ccggcaatac gcggcggccg gcatctccca
     3661 cgttcaggtc tggcccgagc cgaatacgct cgacggcatc gccgcatttg aggagatctt
     3721 cgccgccctc gaccacgcac cggtcaaccc gacgcgtggc taaccggtac agacccgacc
     3781 ctccagcgtc acgctcccgg cttcagtccg ctccttgatc tccatcgaca cgctttgacc
     3841 atccatggag aaggtcaccc gcgcgtcgta ggcatcgaga ttcgcgatct ggtcataggc
     3901 cagcgcgaac gtctgctggt caacccactg tccacgcaac ccgaacggga actcgtaacg
     3961 acccggcgcg aggcggtagt tgccgtctag cccgacctct ccgccgacag gtggctgatc
     4021 tccgctgaag gtcaattgga tttgagcaac ggaggtctca ttgaaggtga gcagcagaga
     4081 ggccaagtcg agcgggtttg gtgcgaagca gaattcgacg cccgagatct tcgcggctac
     4141 cggcggcaat ggcttgaccg gttgcggctc tcgaggttgg gctacgacgc gcacagcctc
     4201 actgagttcc ttttctcccg caggattggc tggcagtgtc ttggttgggt caaccagggc
     4261 gggagtaatc aggtctgttg cctgacccgg atcaataccc gctgcggtaa taacaatctc
     4321 agcacccagt gacgggaaga tctggatttg ctgcccgccg cgtccctgag ccgaaatctc
     4381 gccaccgaca tcactttcgc gcgcgatgta ccagccgtag ccgtagtcat cgtcctcgtc
     4441 tgccggtcgt ttggcatgaa cggatgaagc atccgcgacc catgctttcg agaccacccg
     4501 tgtgccgttc caatcgccct ggttcagcca gagctggccg atctttgccg cgtcgcgcgg
     4561 ttgcagatag aggtcacccc aaccgtgggt gaagccctgc gaatcggctg gccagacaac
     4621 attcgtgatg cctagcggac cgaacaggta cttctttgcg aagtccagtt ccgacatgcc
     4681 cgaagccttg gtcagaacgg ccgaaagcag gtgcatgttc gggctgcaat aggtgtattc
     4741 ggtacccggg tcggacgaca tcggcagatc aagtgcgaac tggacgtagt ccaggctgac
     4801 ttccatctcg gccagcgtct gctcgttcgg ctcagcgata catttgaggc cactggtcat
     4861 gctggcaaga tcgccgacgg tcatcttctc cttccgttca tcgcgatttg cgatggtgcg
     4921 atcggggaaa aaggacagca ccgggtcgtc cagcgacagg agattctggt cgatcgcgat
     4981 cccaacaagc gttgtcgtta cgctcttggt gaccgacgcc aggtcatgcg gatcggcccc
     5041 gtcatacgga tagaagtacg cgtcgagcag gatcttgtcg ttccgcgcga tcaggagact
     5101 gtggatcggc atcccgctgt cccggattgc cagcaaccct ttggccaggg cggcggaatc
     5161 caacccctgc gactcgggtg ttgtggtttg ccagtcgctg gcaaccggag tctgtgcgtg
     5221 agtggtcggg acagccagaa gagtcgccga agcaaggaga atcgcgataa tggtgcgcca
     5281 catgaccgga ctcctttccg gatgtcattc ggtgggaaag cggacacggc actatacgga
     5341 tgcgacagac actcacaagg ctagtaccgc atggtctcac tggtggctga cttaccgatc
     5401 cgggcatcgc aaaaccgcca cagtttgacg ttggagcaag tcccggtcct gtacaacaat
     5461 gccgtctctg ccattcgcaa
//
LOCUS       DATAOZ010000106         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6093530, whole genome shotgun sequence.
ACCESSION   DATAOZ010000106 DATAOZ010000000
VERSION     DATAOZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6093530"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..977
                     /locus_tag="VHR64_03010"
     CDS             324..977
                     /locus_tag="VHR64_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3301824.1"
                     /translation="MPEVIFTGPAGRIEGRYHPAPQKNAPLAIVLHPHPQFGGTMNHQ
                     IIYQMYYAFVHRGFAAIRFNFRGVGRSQGSFDHGTGELSDAASALDWAQSINPEARSC
                     WIAGFSFGAWIGMQLLMRRPEIEGFISVAPPANLYDFSFLAPCPSSGLIVHGDKDAVV
                     PHKDVTGLVDKLKTQKGIIIEQKVIPGANHFFDGKVEPLMQSVAAYLDKRLKGDRRD"
     gene            1021..>1956
                     /gene="tyrS"
                     /locus_tag="VHR64_03015"
     CDS             1021..>1956
                     /gene="tyrS"
                     /locus_tag="VHR64_03015"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX3301825.1"
                     /translation="MSAYKSDFLNVLAERGFIHQVSEPDALDQLANSQPITAYIGFDC
                     TAASLHVGSLLPIMLLYWLQQTGHRPIALMGGGTTRVGDPSGKDESRRILTDDMINEN
                     LKGIREIFSKFLKFEGAGGNAVMANNADWLNSLNYIDFLRDVGRHFSVNRMLSFDSVK
                     MRLDRQQELSFLEFNYMILQAYDFVELNKRTGCVLQMGGSDQWGNIVSGIDLGRRMSN
                     VQLFALTSPLITTSSGAKMGKTAAGAVWLNADLVSPYDYWQYWRNTEDGDVARFLKLF
                     TVLPLDEIGRLSALHGAETNEAKKVLATEATALVHG"
BASE COUNT          366 a          645 c          583 g          362 t
ORIGIN      
        1 gccgcagtgg ttcggtcgcg ttccagtcga gataggcctg cgtcggcatc aaatcctcat
       61 ctgggtgcaa ggatatttca tccaagcccc tgaccatgct cattttactt gcttttggca
      121 cccgggatca tgttagaaac cggcccgccc accgagcgac tccgccttcg acgcggttgc
      181 gccggtcagc ggaactttcg agattcacga atagttctaa tcagttacgt ccgcacctca
      241 ttgccgtcaa ggatgccctg atccgcttcc attgtggcgc tttgctgctg acggaccacc
      301 gccgaccgag gattgaaacg ctcatgcctg aagtcatctt caccggaccg gccggccgca
      361 tcgaaggccg ctatcacccg gcgccgcaga aaaacgcgcc actcgccatc gtgctgcatc
      421 cgcatccgca gttcggcggc accatgaacc atcagatcat ctaccagatg tactatgcgt
      481 tcgtgcaccg cggcttcgcc gcgatccgtt tcaattttcg cggcgtcgga cgcagccaag
      541 gctcgttcga tcacggcacc ggcgaattgt cggacgccgc gtcggcgctc gactgggcgc
      601 agtcgatcaa ccccgaggcg cgctcgtgct ggatcgccgg cttctcgttc ggcgcctgga
      661 tcggcatgca gctcctgatg cggcggccgg agatcgaagg cttcatttcg gtcgcgccgc
      721 ccgccaacct ctatgacttc tcgttcctgg cgccctgtcc gtcgtccggc ctgatcgtgc
      781 acggcgacaa ggacgcggtg gtgccgcaca aggacgtcac cggcctggtc gacaagctca
      841 agacccagaa gggcatcatc atcgagcaga aggtgatccc gggcgccaac cacttcttcg
      901 acggcaaggt cgagccgctg atgcagtcgg tcgccgccta tctcgacaag cggctcaagg
      961 gcgaccggcg ggactaggat ctcgtcaccg ccggcatcgc cgccgcgaag aggaccgccg
     1021 atgagcgcct acaagtccga tttcctgaac gttctggcgg agcgcggctt catccaccag
     1081 gtttccgagc cggacgcgct cgaccagctc gcaaactcgc agccgatcac cgcctatatc
     1141 ggtttcgact gcacggccgc ctcgctgcat gtcggctcgc tgcttccgat catgctgctg
     1201 tattggctgc aacagaccgg tcaccggccg atcgcgctga tgggcggagg cacgacgcga
     1261 gtcggcgacc cgtcgggcaa ggacgaaagc cggcggatcc tgaccgacga catgatcaac
     1321 gagaatctca agggcatccg cgagatcttc tcgaagttcc tgaagtttga gggagcgggc
     1381 ggcaatgccg tcatggccaa caacgccgac tggctcaatt cactgaacta catcgatttc
     1441 ctgcgcgacg tcggccggca cttctcggtc aaccgcatgc tgagcttcga ctcggtgaag
     1501 atgcggctgg accgccagca ggagctgtcg ttcctggaat tcaactacat gatcctgcag
     1561 gcctacgact tcgtcgaact gaacaagcgc accggctgcg tgctgcagat gggcggctcc
     1621 gaccagtggg gcaacatcgt cagcggcatc gacctcggcc ggcggatgtc gaacgtgcaa
     1681 ctgttcgcgc tgacctcgcc gctgatcacc acatcgtccg gcgccaagat gggcaagacc
     1741 gcggccgggg ctgtatggct caatgccgac ctggtgagcc catacgacta ctggcaatac
     1801 tggcgcaaca ccgaggacgg cgacgtggcg cgcttcctca agctgttcac cgtactgccg
     1861 ctcgacgaga tcggccggct ttcggcgctg cacggcgccg agacgaacga ggcgaagaag
     1921 gtgctggcca ccgaagcgac cgctttggtg catggc
//
LOCUS       DATAOZ010000107         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6358256, whole genome shotgun sequence.
ACCESSION   DATAOZ010000107 DATAOZ010000000
VERSION     DATAOZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6358256"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VHR64_03020"
     CDS             <1..327
                     /locus_tag="VHR64_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301826.1"
                     /translation="GGTAGALIVMSTQHRYFVGLGVGLGFNAVLVAMIANYRALPVAI
                     WSIIFSLLSIGGNGVEYSTGIPAEVVQVVLAVVILFIAVRQGMLNALFGRLRAVGARF
                     SPGKAR"
     gene            331..1257
                     /locus_tag="VHR64_03025"
     CDS             331..1257
                     /locus_tag="VHR64_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301827.1"
                     /translation="MDVAQFVITSALTLSVPIIFVALGGMWSLTVGELNIGLEGQMLI
                     GAFISVTVAYATGSTLVGVVSGGIAGIVSGLLFGVLVVYLKGDPFIVGITTNILFSAL
                     TVMLLGLLFRVKGSFSDPSLKSIARFPVGPIADVPIIGRILGDQSVMFGVMILAVAAS
                     WYVLRFTTLGLRLRIAGEKPEALLTAGIRVPRMRLVVQLVTGLLCGLGGAELAMGSLT
                     LFSTDMTAGRGFVAIGIVIIVGSRPVILAVSTLGYGILASIGLDLQQTGFPPQIPQML
                     PYLAAIVAMVLIAVRRTRRNRQVTLDTRLATP"
     gene            complement(1258..>1684)
                     /locus_tag="VHR64_03030"
     CDS             complement(1258..>1684)
                     /locus_tag="VHR64_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301828.1"
                     /translation="PHAGELNGPEAIDHAVTRLHALRVQHGITVLTDPTLVARLRAAE
                     VCFDVCPTSNFLLSLVESIEEHPLPRMLDAGLRCSINADDPVIFRTSILNEYELCRDS
                     LGLTDLHLADCARNSISFSSATAATKQHAFAQIDSWIRG"
BASE COUNT          272 a          514 c          537 g          361 t
ORIGIN      
        1 ggcggaacgg cgggcgcgct catcgtcatg tcgacacaac accggtactt cgttggactc
       61 ggggtcgggc tcgggttcaa cgccgtcctg gtcgcgatga tcgccaacta ccgcgcattg
      121 ccggtcgcga tctggtcgat catcttctcg ctgctctcca tcggcggaaa cggggtcgag
      181 tacagcaccg gcataccggc cgaggtcgtg caagtcgtgc tcgcggtcgt cattctcttc
      241 atcgccgttc gccaggggat gctgaacgcc ctcttcggcc ggctccgcgc cgtcggcgcc
      301 cgcttcagtc ctggaaaggc acgatgacca gtggacgtcg cacagttcgt catcacctcg
      361 gcgctcaccc tgagcgtccc catcatcttc gtggcgttgg gaggaatgtg gtctctcacg
      421 gtcggcgaac tcaacatcgg actcgaaggc cagatgctga tcggcgcctt catctcggtg
      481 accgtcgctt acgcaacggg atccacgctt gttggggtgg tctccggggg catcgccggc
      541 atcgtctccg ggctgctctt cggcgtgctc gtcgtgtacc tcaagggtga cccgttcatc
      601 gtcggcatca ccacgaacat cttgttcagc gccttgacgg tgatgctgct cgggctgctg
      661 ttcagggtga agggctcgtt cagtgacccg agcctcaaat cgatcgccag gtttccggtg
      721 ggaccgatcg cggacgttcc gatcatcggg cggattctcg gcgaccagtc cgtgatgttt
      781 ggggtcatga ttctggcggt agccgcttcc tggtacgtgc tgcgcttcac caccttgggt
      841 cttcggctcc gcatcgcggg ggagaagccc gaagctctgc tcactgccgg tattcgagtg
      901 cccaggatgc gcctggtcgt gcaactggtc accgggttgc tgtgtggcct gggcggagcg
      961 gagctcgcta tgggaagcct taccctcttc tccacggaca tgacggccgg tcgcggcttt
     1021 gtcgccatcg gcatcgtgat catcgtggga tctcgcccgg tgatactcgc cgtgtccact
     1081 ctgggctacg gaatcctcgc gagcatcggg ctggatctgc agcagaccgg tttcccgccc
     1141 cagatcccgc agatgctccc gtacctcgcc gccattgtcg ccatggtttt gatcgcggtt
     1201 cgccggacgc ggcgaaaccg tcaggtcact ctcgacaccc gactcgccac gccatagcta
     1261 accacgaatc cagctgtcga tttgcgcgaa ggcgtgctgc tttgtcgccg cagtcgcgct
     1321 gctgaaggaa atcgagttgc gggcgcagtc cgcaagatga aggtcggtga gaccgagtga
     1381 atctcggcag agttcgtatt cgttcaatat cgaggtgcga aagatcactg ggtcatcggc
     1441 gttgatggaa caccggagcc cggcatcgag cattcttggc aatggatgct cttcgatgct
     1501 ctcgacaaga gaaagcagga aattcgaggt gggacagacg tcgaagcaca cctcggcagc
     1561 tcgcagcctg gcgacgaggg tagggtcagt gaggactgtg atgccatgct ggactcgtaa
     1621 ggcgtgcagt cgcgtaaccg cgtggtcgat cgcttcgggc ccgttcagtt cgccggcgtg
     1681 cggg
//
LOCUS       DATAOZ010000108         5072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1887685, whole genome shotgun sequence.
ACCESSION   DATAOZ010000108 DATAOZ010000000
VERSION     DATAOZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887685"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VHR64_03035"
     CDS             complement(<1..434)
                     /locus_tag="VHR64_03035"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX3301829.1"
                     /translation="MYKGQKSIVLASASPRRRDLIRHLGIAVEIDPSHVPEDTESHVP
                     PEAMVAELAVRKAAEIVARRPDSIVIGADTTVEIDGVILNKPADLADAERMLRRLRGR
                     THRVNTGVAVLAPAQTTHAEVMTSRVTMRPFPDTELHAYLETG"
     gene            570..1895
                     /locus_tag="VHR64_03040"
     CDS             570..1895
                     /locus_tag="VHR64_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX3301830.1"
                     /translation="MSDLTIATIIFVVTYAVIISERLERAVVALAGAALMIAFGVLNQ
                     HQALESIDPNTLALLIGMMIIVGILKRTGVFRYVGWQTAIALHGDPWKMMLGFALFTA
                     VASAFLDNVTTILLMVPVTIAICDDLGLDPRPFMITQVIASNIGGTATLIGDPPNILI
                     GSATGLDFMAFLTNLAPVVVVLLALMVAGFWLVFRRGDRLGAPTPIGMKALASVDARG
                     HIADPALLRRSLVVLALTIAGFVLHSVLNYEAGTIAMFGAVLLLLISRIELHPILVEV
                     EWPTIFFFIGLFILVGGIEQVGLLDKIARETVSLTGGRVALTALVLLWFAGIASALVD
                     NIPAVATLIPLTFSVTRLLFPDLAHLSDADFALHPLVTPFWWSLALGACLGGNGTLVG
                     ASANVVAVGIAERRNESIGFWGFTKLGAPFALMSLAVSSLYVWLRYFAV"
     gene            2375..3028
                     /locus_tag="VHR64_03045"
     CDS             2375..3028
                     /locus_tag="VHR64_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3301831.1"
                     /translation="MNRALLVDDHAVYREAFGLVLEQRLDVRVAAQVASIDEARPWFA
                     DVELGVVGLSISNGSCATLLREWRVANPAVPILVLSESPERSDLAMAIEAGATGVLPK
                     STSLAEIIESIQLAWAGESLLPPQEMIDLLRMASLRRENEYAAQETLHRLTPRELEVL
                     QVLAEGLSDREIGERLHISTETVRTHFVNILGKLHVSSRIQALVFAVRHGAVQIRPE"
     gene            3281..4468
                     /locus_tag="VHR64_03050"
     CDS             3281..4468
                     /locus_tag="VHR64_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX3301832.1"
                     /translation="MLPSVVQPGLEAPDRPRALIDDVELPPPVSIGEGSGFAALVASM
                     LRCRRCSILLTDSEGFLTLEDAVGLPASARRMRIPMGRGIAGRVAAAGTPLVVGDETE
                     ALPDHTPSGAYRTASFLSVPFVLADGRIGVMNATERESGEAFGQADLDLLGRLASFFA
                     SSSYGQSARHEAALTAELRRTRELQIRIQEDERQRLARDLHDDAGHRILAAVLRLDVA
                     SQHCLESVSIRHELDEVRQLLTECADGLHEVAFGLRPQILTDLGLAAAIRSLTARLQG
                     ATGVCIRVLDGRLTQRPAEEVELAAFRIAQEALSNAVKYAQATLVKVSIRQTDDRFVL
                     SVADNGVGFEPAVIDSSSRPRLGLRGMRERAELVGGSLDVRSRPGFGTSVRAQFPVSE
                     VTS"
     gene            4543..>5072
                     /locus_tag="VHR64_03055"
     CDS             4543..>5072
                     /locus_tag="VHR64_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3301833.1"
                     /translation="MLEQDGRLRVIGEASGGREAVRLASDLRPDIILMDINLPDISGL
                     EATRQIKANSPGTRVLVVSLYKESEYVLGMLEAGADGYLVKHCDPVELRGGVLRAHAG
                     ERVLHQSVLHAVIAKAVNGPAAMTAEALSQRECEVLQLLAEGATSKEIALALGLRPKT
                     VENHRARILDKLGVTN"
BASE COUNT          853 a         1516 c         1675 g         1028 t
ORIGIN      
        1 ccggtctcca gataggcgtg caactcggtg tcgggaaacg gccgcatcgt cacccgtgac
       61 gtcatgacct cggcatgggt cgtttgagcc ggcgcgagga ccgcgacgcc ggtgttcacg
      121 cgatgcgtac gccctcgcaa ccggcgcagc atccgctcgg catccgcgag atcggccggc
      181 ttgttcagga ttacgccgtc gatctcgacc gtggtgtccg ccccaatcac gatggagtct
      241 ggtcgccggg ccacgatctc ggctgccttg cgcaccgcga gctcggcaac cattgcctcg
      301 ggcggaacgt gggactcggt gtcttcaggg acgtgggagg gatcaatctc gaccgcgatg
      361 cccaggtgac ggatcagatc gcgccggcga ggggaggcgg aggcaagaac gatcgatttc
      421 tggcccttgt acatcaacgt atactcatcc cggttctgat gcgatcacgt aaccggggtc
      481 gccgcgcatc ccgtacccgc gcggctatgg aggggactgc caatcggcgg tccctttttt
      541 gttccgtgtt atgagccgcc gggagcgcca tgtcggatct gaccatcgcg accatcatct
      601 tcgtcgtcac ctacgcggtc attatcagcg aacggctgga gcgagcggtc gtcgcactgg
      661 ccggggcggc tttgatgatc gcgttcggcg tcctgaacca gcatcaggcg cttgagtcaa
      721 tcgatccgaa cacgctggcg ctgctgatcg ggatgatgat catcgtcggc atcctcaagc
      781 gcaccggggt cttccgctac gtaggctggc aaaccgcgat tgccctgcac ggcgacccgt
      841 ggaagatgat gctcggcttt gcgctgttca ctgcggtagc gtcggcattc ctggacaacg
      901 tcaccaccat cctcctgatg gtcccggtca cgatcgcgat ctgcgacgac ctcggtctcg
      961 acccgcgccc gttcatgatc acgcaggtca tcgccagcaa catcggcggg accgcgacac
     1021 tgattggcga cccgccgaac atcctgatcg gcagcgcgac ggggctcgac ttcatggcct
     1081 tcctgaccaa cctggcgccg gttgttgtgg ttctgctggc attgatggtt gccgggttct
     1141 ggttggtctt caggaggggt gatcgactcg gtgcgccgac cccgatcggg atgaaggcgc
     1201 tggcgtcggt cgatgcgcgt gggcacatcg cagatcctgc gctactgcgg cgatcgctgg
     1261 tggtgcttgc gttgacgatc gccggcttcg tgcttcacag cgtgcttaat tacgaggccg
     1321 ggacgatcgc catgttcggc gcggtactcc tcctgctgat cagccggatc gaactgcatc
     1381 cgatcctggt cgaggtggaa tggccgacca tcttcttctt cattgggctg ttcattctgg
     1441 tcggcgggat cgagcaggtg gggttgctgg acaagatcgc acgggagacg gtctcgctga
     1501 ccggaggccg agtggccctg accgcgctcg tgctgctctg gttcgcgggg atcgcatccg
     1561 cgctggtcga caatatcccg gccgtggcca cgctgatccc gttgacgttc tcggtcacgc
     1621 ggctgctctt ccccgatctg gcgcacctca gcgatgccga tttcgcgttg cacccactgg
     1681 tcacgccgtt ctggtggtcg ctggcgcttg gggcctgcct gggcgggaac ggcacgctgg
     1741 tcggagcatc cgccaacgtc gtcgcggtcg gaattgccga gcgacggaac gagtcgatcg
     1801 ggttctgggg attcacgaag ctcggtgcgc catttgcgct gatgagcctc gcagtctcga
     1861 gcctctacgt ttggctgcgc tatttcgcgg tctagatagt ggtcagggct tgcggcgcca
     1921 gcccggccag ccggaggaat ccgtggaacc tccgtcggac ggatcgggct gaggtgacgt
     1981 tggcgtcgga gtgggggatg gaacggctgg cccggagatc ttggtcccgc attccgggca
     2041 gaagcggtcc tggacccaca ggcgctcgcc gcaggttggg caattcgtga tccggccgct
     2101 ctcgcttggc tgattcggct tcatggcatc aaaggattga tcaccggcgt acccgttagc
     2161 aaggtctggc tcgacggttt ctcccgcgag tacggataaa tgtatacgag agtcgttgac
     2221 atagtcgcgt gcaatccctg atattcgatt caggtattga acgatcttag ccgtgtgctc
     2281 gtcggatcga cgagctattt gtgcatagcc gcacgctggc tgcgcgcccg tttagtgacg
     2341 gtggatcgtt ggagcgcctt gaggaggtaa ccagatgaac cgcgccctcc ttgtggacga
     2401 ccacgctgtc tatcgcgagg ccttcgggct ggtgctggag cagcggctcg acgtgcgggt
     2461 tgcggcgcag gtcgcatcga ttgacgaggc acggccctgg ttcgccgacg ttgagctggg
     2521 cgtcgtggga ctctcgatca gcaatggcag ctgcgccacg ttgctgcgtg agtggagagt
     2581 ggcgaatccg gcagttccca ttctcgtgtt gagcgaaagt ccagagcgat ccgatctcgc
     2641 gatggcgatc gaagcgggtg cgaccggtgt gttgccgaaa tcaacctcac ttgcggagat
     2701 aatcgagtcg atccagcttg cctgggctgg tgagtccctg cttccacccc aggagatgat
     2761 cgacctcttg cgcatggcaa gcctgcgtcg cgagaacgag tatgccgccc aggaaacctt
     2821 gcaccgtctg acgcctcgcg agctcgaggt tttgcaggtt ctggccgagg gactgagcga
     2881 ccgggagatc ggggagcggc ttcatatcag cactgaaacc gtgcgcacgc acttcgtcaa
     2941 catcctcggc aagctccatg tcagctcacg gatccaggcg ctggttttcg ccgttcggca
     3001 cggagcggtc caaataaggc cggaatagac ccgttgccca tttggcacag ggatttactt
     3061 ttgtaccaat gtacgtatac tgcaattgtg gtacttagaa atacccagaa ttgatgagac
     3121 ataatacctc agatagggct atgatcaata cagatagccc aaaagtaccg tacggatgga
     3181 gaccaatgtc gtgacgctcg tggccggggg ttgccggccg catcttcggc gtcctggctt
     3241 tgcttgcgct tcttcgggtt cgaggtgagg tggtttcgag atgttgccgt ccgttgttca
     3301 gcctggtctc gaggctcctg acagaccccg cgcgctcatc gacgacgtgg agttaccgcc
     3361 gcctgtctcg ataggcgaag gctcaggatt cgccgctctg gtggcatcga tgttgcgatg
     3421 tcgccgatgc tcgatcctgc tgaccgacag tgaagggttc ctgactctcg aagacgcggt
     3481 tggtctgcct gcctccgcga ggcgaatgcg gataccgatg ggccgaggca tcgcgggcag
     3541 ggttgccgca gcaggaacgc cactcgtagt cggtgacgag accgaggcgc ttccggatca
     3601 cacgccgagc ggggcgtacc gcaccgcgtc gttcctgtct gtaccgttcg ttctggcgga
     3661 tggtcggatc ggtgttatga acgcgactga gcgcgagtcc ggggaagcat tcggccaggc
     3721 cgatctcgac ctgctgggcc ggttggcgtc atttttcgca tcctcctcgt acgggcagtc
     3781 ggcgcgacac gaagccgccc tgactgcgga gttgcggcga acgcgcgagc tccagatccg
     3841 gatccaggag gacgagcggc aacgactggc gcgcgacctg cacgacgatg ccggccatcg
     3901 cattctcgcc gcagtcctgc gcctcgatgt cgcctcgcaa cattgcctcg agtccgtatc
     3961 catccgccat gagcttgacg aggtgcggca attgctgacc gagtgcgccg atggcttgca
     4021 cgaggtagcg ttcggccttc gcccgcaaat cctgaccgat ctcggtctgg cggcagccat
     4081 ccgatccctg accgcccggc ttcagggcgc gaccggcgtt tgcatccgag tcctggacgg
     4141 ccgtctgacg cagcgcccgg cagaagaggt cgagctggcc gcgtttcgca tcgcccagga
     4201 ggcgttgagc aacgccgtca agtacgcgca ggccaccttg gtcaaggtca gcattcgcca
     4261 gactgatgac cggttcgttc tttcagttgc cgacaatggt gtcggatttg agcccgcggt
     4321 gattgattcc tcgtcgcgac cccgcctcgg tctgcgagga atgcgagagc gggctgaact
     4381 ggtcggtggt tcgctggatg tgcgatcccg gccgggtttt ggtaccagtg ttcgggcgca
     4441 gttcccggtg agtgaggtga catcatgacg gactcgcggc gaggccaggt gagggtattg
     4501 ctggtcgatg atcacccaat tgtgcgaaca ggaatgcgat acgtgctgga gcaggacggt
     4561 cgcctgcgcg tgatcggcga ggccagcggg ggccgcgaag cggtccggct ggcaagcgac
     4621 cttcgccccg atatcatcct gatggacatc aacctgccgg atatcagcgg tctcgaggcc
     4681 acccgccaaa tcaaggccaa tagccctgga acccgggtgc tggtcgtctc gctctacaag
     4741 gaatcggagt atgtccttgg gatgctcgag gcaggcgccg acggctacct cgtcaagcat
     4801 tgcgatccgg tggagctgag aggcggcgtg ctgcgggcgc acgccggcga gcgtgtcctg
     4861 catcagagcg tgctgcacgc ggtcatcgcc aaggcagtga acggtcccgc ggcaatgacg
     4921 gccgaggcgc tcagccagcg cgagtgcgaa gtgctgcaac tcctggccga aggtgcgacc
     4981 agcaaggaga tcgcccttgc gctcggcttg cggccgaaga cggtcgagaa ccaccgggcc
     5041 cggatcctgg acaaactggg cgtcaccaac ag
//
LOCUS       DATAOZ010000109         4275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2412723, whole genome shotgun sequence.
ACCESSION   DATAOZ010000109 DATAOZ010000000
VERSION     DATAOZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2412723"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..672
                     /locus_tag="VHR64_03060"
     CDS             250..672
                     /locus_tag="VHR64_03060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX3301834.1"
                     /translation="MFLIDTSTEFGKRVARQLEEEEILWLTTVDANGVPQPVPIWFLW
                     DGETILFYSQPNKPKLRNIERNSRVSLNFNTEPMGEEVGVFIGTLAVDPTAPLANQSP
                     EYQKKYGDGIIRIGYTNDTYSETWSVPLRFTPEKLRGQ"
     gene            835..1032
                     /locus_tag="VHR64_03065"
     CDS             835..1032
                     /locus_tag="VHR64_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301835.1"
                     /translation="MSGCALFLVWAGCVLLGAVVGFFVGWILWKLGFEIIGSAVALVG
                     AGAGGIVAFFAFLNWSEDRWR"
     gene            complement(1426..2829)
                     /gene="mnmE"
                     /locus_tag="VHR64_03070"
     CDS             complement(1426..2829)
                     /gene="mnmE"
                     /locus_tag="VHR64_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX3301836.1"
                     /translation="MDNATIAAIATPPGEGGIGMVRISGTEAKDIAGRIFRRGRNGHE
                     VDLTKVASHRLLYGRVVDPASGETIDEVLLGWMAAPHTYTCEDIVELTCHGGLVPLQE
                     TLRVALAAGARHAEPGEFTLRAFLNGRLDLTQAEAVLNVVGARTGEALRLAVDDLSGN
                     LTRRLTPARDAVVSLLAFLDAAADFPEDEIPPTDIDADLATAEEALAEIVAGSRAGML
                     YQEGAQIALVGRPNVGKSSLMNALLRADRAIVTPVAGTTRDVISETINLRGIPATLLD
                     TAGIAETADVVELLGIERSRKALTASAAAVLVLDGSVPPTEQDFAVAQLLVDRLGSDI
                     GPDSAPVVIAVNKRDLPERAAQDDVTRVLPGCPVIAVSCVTSEGIDELEDALAGLLGG
                     AGDARPSLITARQHAVLDRALEHIRNAVEARATGYPIDLLATDVRAALHAIGEVTGEA
                     VDDAVLTEIFSRFCIGK"
     gene            complement(3010..3954)
                     /locus_tag="VHR64_03075"
     CDS             complement(3010..3954)
                     /locus_tag="VHR64_03075"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX3301837.1"
                     /translation="MSDRKLQAGKLLVDVPLVRRLIDTQFPQWSELPIRPVAHDGWDN
                     WTFHLGDDMTVRLPSAAGYAEQVEKEQHWLPRLAPVLPLPIPVPLAMGMPDAEYPWHW
                     SVYEWLDGEVATIERIADLPRFASTLADFLVALQQADATGGPPPGQHNFWRGGPLTVY
                     DDETRQALKTLEGAIDIDAARAVWETALAATWHGSPVWFHGDVAFGNLLVKNGELYGV
                     IDFGTSGVGDPSCDLAIAWTLFAGESREVFRAALPLDEGTWARGRGWTLWKALITLAP
                     IADTDSLEARTAWNVIDEVLADHELTRERSGPGLEVRV"
BASE COUNT          806 a         1388 c         1312 g          769 t
ORIGIN      
        1 aactgcacgg gcactagaac gggcgtttgg agataggaga taggcgttag gcatgaggcg
       61 tgaggcgacc gtgctcagcc ccctcctctt cggtgatctt agcgcgggaa gggtgcttca
      121 gaccgtcgac cgcgcctagg cgatggcctg aagactgtcg ctaggcgcgg ccgttcgccc
      181 tcaacctgtg gccgtcgatc cattccatca tccctaacgc ctaacgccta acgcctgagg
      241 agaaccggga tgtttttgat cgataccagc acggagttcg gcaagcgtgt tgctcgccag
      301 cttgaggaag aggaaatcct ttggctgacc accgtcgacg cgaatggtgt gccccaaccg
      361 gtgccgatct ggtttctctg ggatggcgaa acgatcctct tctacagcca accaaacaag
      421 ccaaaactcc gcaatatcga gcgcaactca cgggtatcgc tcaacttcaa cacagaacca
      481 atgggcgagg aggtcggcgt cttcatcggc acgctcgccg tcgatccaac tgcgcctctc
      541 gccaaccagt cgcctgagta tcagaagaag tatggcgacg gaatcattcg catcgggtac
      601 acgaacgaca cctactccga aacctggtcg gttccgttgc ggttcacacc ggagaaactg
      661 cgcgggcaat agcctaagga gtcgagaacg ccgagacgac gtgcaagacg cgatctctca
      721 agcatcaaac ccacagtcgt caattgcacg ctaagatatg cgcggaacga tagacccatg
      781 gcgccggatt ccaatcactg ggaacggcgt tacctacgcg agcaggagag acccatgagt
      841 ggttgtgccc tcttcctggt ttgggctggt tgtgtgcttc tcggcgccgt ggtcggattc
      901 tttgtcggct ggatactttg gaaactcggt ttcgagatta tcggcagcgc ggtcgccctc
      961 gtcggcgccg gggcgggtgg catcgtcgcg ttcttcgcct tcctcaactg gtccgaagac
     1021 cgctggcgat agctcccccg ggtttgcaac ctggacttga cacgagaaag ccgccgcccg
     1081 gcttaacacg gtggtgcccg acgaccgccc cgtcctcagc cgcgcagcgg cttcgtttgt
     1141 gagcccgggg attgatcccc ggggagccgt cccaacccca actcttcgcg agagccacga
     1201 accctgccca gtcattccga gcggagccga ggaatccgtg ccggccagaa gcatgtggtg
     1261 aatagccaca gctccgtcga ctccggccaa ccctcacccc caacccctct ccccgcgaat
     1321 cagggagagg gtggcccgaa gggccgggtg agggttgggc cgggtgaggg ttgcttaccg
     1381 ggtcagaacc gttccaggta cggatgacgc gcgcaacgcc cgtttctatt tcccgatgca
     1441 gaaccggctg aagatttcgg tgaggacggc gtcgtcgacc gcctccccgg tgacttcgcc
     1501 aattgcgtga agcgcggcac ggacatcggt agcgagcaga tcgatcgggt acccggtggc
     1561 gcgcgcttcc acggcgtttc gaatgtgttc cagagcgcgg tcgagcacgg cgtgctggcg
     1621 cgcggtgatc aacgatgggc gcgcatcgcc agcgccgcca agcagccctg cgagcgcatc
     1681 ctccaactcg tcgatcccct cgctggtcac gcaggaaacc gcgatcaccg gacagccggg
     1741 cagcacacgt gtgacatcat cctgcgccgc acgctccggg agatcccgtt tgttgacggc
     1801 gatcacgacc ggcgcggaat ctggaccaat atcactcccc aatcggtcga ccagaagctg
     1861 cgcgaccgcg aaatcttgtt cggtgggcgg taccgaaccg tcgagcacca gcaccgccgc
     1921 cgctgacgcg gtcaacgcct tgcggctgcg ttcgatgcca agcaactcga cgacatcggc
     1981 cgtttccgcg atgccggccg tatcgaggag cgttgccggg ataccgcgca aattgatcgt
     2041 ctcggagatc acgtcccgtg tcgtgccggc caccggcgtg acgatggcgc ggtcggcccg
     2101 cagcagggcg ttcatcagac tgctcttgcc gacattcggc cgcccgacga gcgcgatctg
     2161 cgccccttct tgataaagca tccccgcccg cgacccggcg acgatctcag ccagcgcttc
     2221 ctcggcggtc gccagatcgg cgtcgatatc ggttggcggg atttcatctt ctggaaaatc
     2281 ggcggccgcg tcgagaaacg ccagcaggga cacgacggcg tcccgcgccg gggtgagacg
     2341 gcgggtcagg ttgccgctga gatcgtcaac cgccagccgt agcgcctcgc cggttcgcgc
     2401 gccgacgaca ttcaacaccg cctcagcttg agtcagatcg agccgcccat tgagaaacgc
     2461 gcgcaaggtg aactcgcctg gttcagcgtg gcgcgccccc gccgcgagcg cgacgcgcag
     2521 cgtctcttgc agcggcacca gaccgccgtg gcacgtgagc tcgacgatgt cctcgcaggt
     2581 gtaagtgtgg ggcgcggcca tccagccaag gagcacctcg tcgatcgtct cgccactcgc
     2641 tggatcgaca actcgtccat agagcaaccg gtgcgaagcg acctttgtca gatcaacctc
     2701 atgcccgttc cgaccccgcc gaaagatgcg gcccgcgata tccttcgcct cggtcccgct
     2761 gatgcggacc atgccgatcc caccctcccc gggcggtgta gcaatagcgg cgatcgtggc
     2821 gttgtccata ggtgtgcgat tctagtttct agcattgata gatcgacgtt cgattgtagt
     2881 gtgtgagaat gggaaaacct acgccctctg cgacgccgcc gggttaaaac cccggcgctg
     2941 aaacgacgaa gtcccaaagg gactcagtga cggcaccacg acccacagtc ccgaagggac
     3001 ttcgtcgtgt tagacccgga cttctagtcc ggggccagag cgttcgcgtg tcaactcatg
     3061 atcagcaagc acttcatcaa ttacgttcca cgctgttcga gcctccaggg aatcagtgtc
     3121 cgcgatcggg gcgagcgtga tcagagcctt ccacagtgtc cagccgcggc cgcgtgccca
     3181 ggtcccttca tcgaggggaa gcgcggcacg gaaaacctcg cggctctccc ctgcgaagag
     3241 cgtccaggcg atcgccaaat cacacgaggg atcacccaca cccgaggtgc cgaagtcgat
     3301 gacgccgtac aattcgccgt tcttgaccag caggttgccg aaggcaacgt cgccgtggaa
     3361 ccacacaggt gagccatgcc aggttgccgc gagcgccgtt tcccagaccg ctctcgccgc
     3421 atcgatatcg atcgcgcctt cgagcgtttt cagcgcttgc cgggtttcgt cgtcgtagac
     3481 ggtcagtggt ccgcctcgcc aaaagttgtg ctggccgggc ggcggtccac ccgtggcatc
     3541 ggcctgttgc aaggcgacga ggaaatccgc cagcgtgctc gcgaaccgag ggagatcggc
     3601 gatgcgctcg atggtcgcga cctcgccgtc gagccactca tagaccgacc aatgccaggg
     3661 atactcggcg tccggcattc ccatggccag ggggacggga atggggagcg ggaggaccgg
     3721 cgccagtctc ggcagccagt gttgctcctt ttccacctgt tcggcgtaac cggcggcgct
     3781 cggcagccgc acggtcatgt catcgccgag gtgaaaggtc cagttgtccc agccatcgtg
     3841 cgcgaccggt ctgatgggaa gttccgacca ttgcggaaat tgcgtgtcga tcaaccggcg
     3901 cacgaggggg acgtcgacca gcaatttccc cgcttgcaac tttctgtcgg acattgagct
     3961 tccttcgcgc cgttttacgc aggggcgcat cgatatgcgc ccgggatcgc attcgctccg
     4021 tggcgctaat gacaaccgcc gtgttcacca cgcgggcgca acacccaaag ggtacccggc
     4081 gccgctacgg aacacgcgat tggccgccat ccacgccgtc ccgggagaat ttgtcctgac
     4141 gcctgacgcc cgtcgccctc aaccccggat cgggtattcc tcagcggttt tgaggaactc
     4201 gtcgacttcc ttggcggtag ggacgccgtc agtgccgata ccgcgcaggc tgagggcagc
     4261 gacagcgttg gcgaa
//
LOCUS       DATAOZ010000110         6983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6095446, whole genome shotgun sequence.
ACCESSION   DATAOZ010000110 DATAOZ010000000
VERSION     DATAOZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6095446"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VHR64_03080"
     CDS             complement(<1..898)
                     /locus_tag="VHR64_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3301838.1"
                     /translation="MAHSLTIVGLGPGDPDLRTIAAQRALDQAPQILLRTGVHPGIEE
                     MLADPRISTCDDLYEQQPTFQALYRAVTDRALALLAETDLVYAVPGNPLAGELTVITL
                     RSAAESTGHEVQVIPGAGGLDVIAATAGLDLMTDGVQTLDALDLRDWLERAPFNGSLL
                     DVSPTRPLIITQVYKRSIAAAVKLSLGTLYPDDHDISIIGWDKESGVTSARLIQLHQL
                     DRAKVDHLTSIVVPALDWQQNTRSPFELFRIVSRLRDLNGCPWDREQTHKSIRGAVIE
                     EAFEVADAIDEGDMAALSDELGD"
     gene            complement(902..2764)
                     /gene="lepA"
                     /locus_tag="VHR64_03085"
     CDS             complement(902..2764)
                     /gene="lepA"
                     /locus_tag="VHR64_03085"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX3301839.1"
                     /translation="MTTKVVPARRQGGAVPLERIRNFSIIAHIDHGKSTLADRLLEAT
                     GTVDRREMVAQVLDSMDLEREKGITIKARAVRMHYTANDGETYELNLIDTPGHVDFSY
                     EVSRSLAACEGAILVVDAAQGIEAQTMANVYLALEQDLALVAVINKIDLPNADPERVA
                     REVSSFIGLLDDEIVPASAKEGRGIPEILEAVVRTVPPPTGDQTAPTRALIFDSHYDS
                     YKGVIAYVKIVDGEIKTDDRIRLINSGTQADVLELGFFGPRMSPTSALTTGEVGYVAT
                     GLKDVKHIQVGDTITLAANPARQPLPGYRPAKPMVFAGLYPVDSDAYPDLRDALDRLA
                     LNDASLTYEPESSLALGFGFRAGFLGLLHMEIIQERLEREYKLDLLATAPSVEYEVVL
                     HGGKQIVIDNPAALPPTGEIEAILEPWMDINIISPTRYIGNIMELVTGRHGTYQEMIY
                     LDEERVQLKFQMPLGELIVDFYDQLKSRTQGYASLDYQFSELREGDLVKLDILVNSAP
                     VDALSIICHRDDAYAKGRDLVSALRELIPRQMFDVPVQASIGSRIIARETIRAMRKNV
                     ISKCYGGDITRKRKLLEKQKEGKARMKIVGSVEIPQEAFMAVLTLGDKSSQTKS"
     gene            complement(2802..4556)
                     /gene="murJ"
                     /locus_tag="VHR64_03090"
     CDS             complement(2802..4556)
                     /gene="murJ"
                     /locus_tag="VHR64_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX3301840.1"
                     /translation="MTAQGNRPSTASPSRQRIPNAGGRLNRPFGVRPYLEPRTTPPVV
                     SFESEPPSTPGVETASATRSAAIIGAAFILSRLLGILRDVLLGHQFGVSAELDAYYAA
                     FRIPDLVFLGVMSVAFGAAFIPVFGGFLARGEEESAWRLASAVIELAMVMTVILCVIA
                     FVLAGPLMRHVIAPDLPADVMPDAIKTMRILLLSPILIGLGIAAKGILEAQDLFTLPA
                     LAPVAYNAAIIFGIITLAPSLGIEGVAIGAVIGAAAHIAVQVPGVFKSGFRFMPASRP
                     FNVAGVVEVMRLLGPRVIGQAAFQINFIWVTSLANRSGEGRVAALNFGWQMLMLPHGL
                     IALSISTVIFPRMARLYEQGKTGEVRHVFTQALTPMLFLTLPASIGLYEFRYAVVKAI
                     FQNGAFTEAGTELVASAIEFLALGLVFYAVVEIATRIFYAMKDTVTPVVAGVIIIVIN
                     MVIGYLLLDSLGHVGLAIGLTASTGVEAIILMAVLRRRIGGLDAEFGAWLAKLLIATA
                     VMTLMAELVTRHLNEALASPTIGKPMTLLLVGYAVALVAATYFVAAWILRIPEVTVMI
                     GRAMGIARRLPVIGPVLR"
     gene            4593..4865
                     /gene="rpsT"
                     /locus_tag="VHR64_03095"
     CDS             4593..4865
                     /gene="rpsT"
                     /locus_tag="VHR64_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX3301841.1"
                     /translation="MANNKQSKKRILINETKRLRNRPYRSAARTYVKKAELAIRAGDQ
                     NNAATAVGDALSMLDRVASKGIIHQNNAARRKSRLMKKFNGMGAEA"
     gene            complement(4914..5921)
                     /locus_tag="VHR64_03100"
     CDS             complement(4914..5921)
                     /locus_tag="VHR64_03100"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301842.1"
                     /translation="MIYLLVGPDRYLLERELKRILAELDPDNLSTTRYDKSASIGEVS
                     SAVATSGFFGSGRVIVAEGVMTRASGAGKAKKTDSEEIEALFKSVAPGNTLILVDPEL
                     GTIPAAVKKAAGSDAIQFGGRVPRGSDLVEWVQGQVSAQGGHIERRNVQRMLDRLFPG
                     SWQEANRNPAYDNPPDLLQLSSEIAKLITAADGKEINARHIDALVPQASQEDLFPFID
                     AVVSGNATAAFKLLSGEETDDDIASRYLNQLGTNAELGQAVAEVRSEIALADAGKQLG
                     GAKPNRLAAIQRTFARTDPRSFADVVIEADRRLKTGKTRSPSEQLQEIIVRQASKTRG
                     R"
     gene            complement(5947..6077)
                     /gene="ssrS"
                     /locus_tag="VHR64_03105"
     ncRNA           complement(5947..6077)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VHR64_03105"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            6174..>6983
                     /locus_tag="VHR64_03110"
     CDS             6174..>6983
                     /locus_tag="VHR64_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX3301843.1"
                     /translation="MVDKRRRVVVTGLGALSPVGNTASESWDAVVAGRSGVATITRFD
                     PADYETRFAGEVKGFDPDAVLGKKESRRMDRHAQLGVAAALEARANAGLVDAEVDPFR
                     VGVLMGTGMGAMETLEQGAETLLTRGPGRIGPFFAPMSLPNMAAGMSAIFLGAKGPCV
                     GTVSACASSAHAIGEGVEMIRRGVVDVVYAGGTEAPVTRLSVAGFGAMGALSKRNDEP
                     ERASRPFDADRDGFVLGEGAAVLVLESLEHAEKRGATILAEVAGYAATDDAN"
BASE COUNT         1237 a         2111 c         2410 g         1225 t
ORIGIN      
        1 ggtcgcccag ctcgtcgctg agcgcggcca tgtcgccctc gtcgatcgcg tcagcgacct
       61 cgaatgcttc ctcgatgacg gcgccacgaa tcgacttgtg ggtctgctcg cggtcccacg
      121 ggcagccgtt gaggtcgcgg agcctggaga cgatccggaa gagctcaaag ggcgagcgcg
      181 tgttctgctg ccagtcgagt gccggcacga cgatgctggt caggtgatcg actttggctc
      241 gatcaagctg gtgcaactgg atcagtcgcg cgctggtgac accactctcc ttgtcccagc
      301 cgatgatcga gatgtcatgg tcgtcgggat agagcgtgcc gagggacagc ttgactgccg
      361 ccgcgatcga gcgcttgtag acctgcgtga tgatcagcgg gcgggtcggg ctgacatcga
      421 gcagggagcc gttgaacggc gcgcgctcaa gccaatcgcg gaggtcgagc gcgtcgagcg
      481 tctgcacgcc gtcggtcata aggtcgagac cggcggtcgc ggcgatcaca tcgaggccac
      541 cggcgccagg gatgacctgg acctcgtgtc cggtcgactc ggcggccgag cggagcgtaa
      601 tcaccgtcaa ctcgccggcc aacggattac ccggcacggc gtagacgagg tcggtttccg
      661 cgagtagtgc gagcgcccga tcggtaaccg cccggtagag cgcctggaac gtcggctgct
      721 gctcgtagag gtcgtcgcat gtgctgatgc gcggatctgc cagcatctct tcgatgcccg
      781 ggtgaactcc cgtgcgcaag agtatctggg gcgcctgatc gagcgcgcgt tgtgcggcaa
      841 tcgtgcggag atcaggatcg ccgggtccga ggccgacgat cgtcagggag tgggccacgg
      901 ctcagctctt ggtctggctg gacttgtcgc cgagcgtcag gacggccatg aaggcctcct
      961 gtgggatctc gaccgagccg acaatcttca tccgcgcctt gccttccttc tgcttctcaa
     1021 gcagcttccg cttgcgcgtg atgtcgccgc cgtaacactt ggagatgacg ttcttgcgca
     1081 tggcccggat cgtctcgcga gcgataatgc ggctgccgat cgaggcctgg accgggacgt
     1141 cgaacatctg gcgcggaatc agctcccgca gcgcggagac gaggtcgcga cccttggcgt
     1201 aggcatcgtc gcggtggcaa atgatcgaga gggcgtcgac cggcgcgctg ttgacgagga
     1261 tgtcgagctt gaccaggtcg ccttcgcgca actcggagaa ctggtagtcg agcgaggcat
     1321 agccctgggt gcggctcttg agctggtcgt agaagtcgac gatcagctcg ccgagcggca
     1381 tctggaactt gagctgcacc cgctcttcat cgaggtagat catctcctgg taggtgccgt
     1441 ggcgaccggt gaccagctcc atgatgttcc cgatgtagcg ggtcggggag atgatattga
     1501 tatccatcca cggctcgagg atggcctcga tctcgcctgt cgggggaagc gccgccgggt
     1561 tgtctatcac gatctgctta ccgccgtgga gcacgacctc atactcgaca ctcggcgcgg
     1621 tcgcgagcag gtccagcttg tactcgcgct ccaatcgttc ctggatgatc tccatgtgca
     1681 ggaggccgag gaaacccgcg cgaaagccga agccgagcgc gagcgacgac tccggctcgt
     1741 aagtcagcga ggcatcattg agggcgagcc gatccagcgc gtcccgcaaa tccgggtagg
     1801 cgtcgctgtc gaccgggtag agaccggcaa agaccattgg cttggccggt cggtagcccg
     1861 gcagcggctg cctggcgggg ttggccgcga gcgtgatcgt atcgccaacc tgaatgtgct
     1921 tgacgtcctt caagccggtc gcgacgtatc cgacctcgcc ggtcgtgagc gcggaggtcg
     1981 ggctcatgcg tggcccgaag aagccgagct cgaggacgtc cgcctgcgtg ccgctgttga
     2041 tcagccggat gcggtcgtcg gtcttgatct cgccgtcgac gatcttcacg taggcgatga
     2101 cgcctttgta ggagtcgtag tggctgtcga agatcagggc gcgggttgga gccgtctggt
     2161 cgccggtcgg tggcggaacg gtccgcacga cggcttcgag aatctcgggg atgccgcgtc
     2221 cttccttggc cgacgccggg acgatctcat cgtcgagcag gccaatgaag gagctgacct
     2281 cacgcgcgac ccgctccgga tcagcgttgg gcaggtcgat cttgttgatc acggccacca
     2341 gggccaagtc ctgctcgagc gcgaggtaga cattggccat cgtctgcgcc tctatgccct
     2401 gcgcggcgtc gacgaccagg attgcgccct cgcaggcggc caggctgcgg ctgacctcgt
     2461 acgagaagtc gacgtggccg ggagtgtcga tcaggttcag ctcgtatgtc tcgccgtcgt
     2521 tcgcggtgta gtgcattcgg acggcgcggg ccttgatcgt gatgcccttc tcgcgctcga
     2581 ggtccatcga atcgagcacc tgcgcgacca tctcgcgacg atcgaccgtc cctgtcgcct
     2641 ccagcaatcg atcggcgagc gtcgatttgc cgtggtcgat gtgggcgatg atcgagaagt
     2701 tgcggatgcg ctcgagtgga acggcgccgc cctgccgccg ggcaggaacg accttggtgg
     2761 tcattgtcat aagctcaagt atacgtgtcc aggggccgaa atcagcgcaa aacaggtcca
     2821 atgacgggga gtcgccgggc gattcccatg gctcgcccta tcatcaccgt cacctccggg
     2881 atccggagaa tccaggcagc gacgaagtag gtcgcggcga ccagcgcaac agcatacccg
     2941 accaacagca gcgtcatggg cttgccgatg gtcgggctgg caagggcctc gttcaagtgg
     3001 cgggtgacca gctcggccat cagggtcatg acggccgtcg caatcagcag cttcgcgagc
     3061 caggcgccaa attccgcatc cagcccgccg atccggcgtc gcaggacggc catcaggatg
     3121 atcgcctcga cgcccgtgga tgccgtgagg ccgattgcca ggccaacgtg gccgagcgaa
     3181 tcgagcagga gatagccgat gaccatgttg atgacgatga tgatgacgcc cgcgacgacc
     3241 ggcgtgaccg tgtccttcat cgcgtagaag atgcgtgtcg cgatttcgac gaccgcgtag
     3301 aagaccaggc cgagcgccag gaactcgatc gcggacgcca ccagctccgt accggcctcg
     3361 gtaaaggcac cgttctggaa gatcgccttg acgaccgcat agcggaactc gtagaggccg
     3421 atcgaggccg gcagagtcag gaacagcatc ggggtcagcg cctgcgtgaa gacatggcgc
     3481 acctctccgg tcttgccttg ctcgtaaagg cgagccatgc gggggaagat caccgtggag
     3541 atgctgagcg cgatcaggcc gtgcggcagc atcagcatct gccagccgaa attgagtgcg
     3601 gcgacccggc cttcgccgga gcggtttgcg aggctcgtga cccagatgaa gttgatctgg
     3661 aacgcggcct ggccgatgac gcgcggaccg agcaaccgca tgacttcgac gacgccggca
     3721 acgttgaagg gccgcgacgc cggcatgaac cggaagccgg acttgaagac gcccgggacc
     3781 tggacggcaa tatgagcggc cgcgccgatc accgcgccga tcgcgacccc ctcgatgccc
     3841 agcgacgggg cgagagtgat gatgccgaag atgatggccg cgttgtaggc gaccggggcg
     3901 agggcaggaa gcgtgaatag atcctgcgcc tcgaggatcc ccttggcggc gatcccaagt
     3961 ccgatcagga tgggcgagag gagcagaatg cgcatggtct tgatcgcgtc cggcatgaca
     4021 tcggccggca gatccggcgc gatcacgtgt cgcatcaatg gtccggccag gacgaaggcg
     4081 atgacgcaga ggatcacggt catgaccatg gccagctcga tgaccgccga ggccaggcgc
     4141 caggccgact cctcttctcc ccgggccagg aagccgccga agaccgggat gaaggccgcg
     4201 ccgaaggcga ccgacatcac gcccagaaat acgaggtcgg gaatgcggaa ggcggcgtag
     4261 taggcatcca gctccgcgct gacgccgaac tggtgcccga gcaggacgtc gcggaggatg
     4321 ccgagcagcc ggctgaggat gaaagcggcg ccgatgatgg ccgcgctgcg ggtggcggag
     4381 gcagtttcga cgcccggcgt ggacggcggc tctgactcga atgagacgac gggtggagtc
     4441 gtgcgtggct cgagataggg gcgaacgccg aacgggcggt tgagccggcc gccggcattt
     4501 ggtattcttt gccggctagg gctggccgtt gacggccggt tgccctgtgc tgtcacgcga
     4561 actcagccct gtctttcgaa aggtaatgga ccttggccaa taacaagcag tcgaagaagc
     4621 ggattctgat caacgaaacg aagcggctcc gcaatcgccc atatcgatcc gccgcgcgca
     4681 cctacgtcaa gaaggcggaa cttgcgatcc gcgctggcga tcagaacaac gccgccaccg
     4741 cagtaggcga tgccctgagc atgctcgatc gcgtggcaag caagggtatc atccaccaga
     4801 acaatgctgc acggcgaaag tcccgcctga tgaagaaatt caacgggatg ggcgcggagg
     4861 cttagggagt cggcagactt cggatttcag acgtcggttc ggcgcgcggt cggttagcgg
     4921 ccgcgcgttt tgcttgcctg gcggacgatg atctcctgga gctgctccga tggggagcgg
     4981 gtcttgccgg tctttaggcg gcggtcggct tctatgacaa cgtccgcaaa cgaccggggg
     5041 tctgtccggg cgaacgtcct ctggattgcg gcgagacggt tcggcttcgc gccgccgagt
     5101 tgcttaccgg cgtccgctag cgcaatttca cttcgtactt cggcgactgc ctgtcccaat
     5161 tccgcatttg taccgagttg attcagatac ctcgatgcga tgtcgtcatc cgtttcctcg
     5221 cccgagagga gcttgaatgc cgccgtggcg ttgccgctga ccaccgcgtc gatgaaaggg
     5281 aacaggtctt cttgcgatgc ctgcgggacg agcgcatcga tgtggcgcgc gttgatctcc
     5341 ttgccgtcgg ccgcggtgat cagcttcgcg atctcgctgg agagctggag caggtccggc
     5401 gggttgtcgt aggccgggtt gcggttcgct tcttgccagg aaccgggaaa cagccggtcg
     5461 agcatccgct gcacgttgcg gcgctcaatg tgcccgccct gggcactgac ctggccttgc
     5521 acccactcga cgagatcaga gccgcgggga acccgtccgc cgaactggat cgcatcggag
     5581 ccggctgcct tcttcaccgc cgcggggatc gtgccgagtt ctgggtcgac caggatcagc
     5641 gtgttgccgg gcgcgactga tttgaagagc gcttcgatct cttcggagtc cgtctttttg
     5701 gccttgccgg cgcctgacgc gcgcgtcatc acgccttcgg ccacgatcac gcggccggag
     5761 ccgaagaagc cggaggtcgc gaccgcgctg ctgacctcgc cgatcgaggc cgatttgtca
     5821 taacgagtgg tactcaggtt gtcgggatca agctcggcga ggatacgttt gagctcgcgc
     5881 tcgagcaggt agcgatcggg accgacgagg agatagatca ttgcgctccg ccaggggtgt
     5941 ggtgtcagtg aacccgcgag tgccgtgtgt ctacggagcc ttgaacctgc tctcgcaggt
     6001 gggtaccgcg tatgcagccc cgactcattt ctatcggctc aagcccgcgc agagcatcac
     6061 gaacaccgca ggctcaccac cggttgagcc tagcatgccg gcaggggcag gacaaccaaa
     6121 gcagcgtggt agcatcactc tcttgatacg gcgattcgcg ccacgaggga cgcatggtgg
     6181 acaaacgacg acgggtagtg gttacggggc tcggcgcgct gagcccggtc ggaaatacgg
     6241 cgagcgagag ctgggatgca gtcgtggccg gccggtccgg agttgcgact atcacccgat
     6301 tcgatccggc cgactacgaa acccgattcg ccggcgaggt caagggcttc gacccggacg
     6361 cggtgctggg caagaaggaa tcccgccgca tggaccgcca cgcgcaactc ggtgtcgcag
     6421 cggcgctgga agcccgggcg aatgccggcc tcgtcgatgc ggaggttgat cctttccggg
     6481 tcggcgtgct gatgggcacg ggcatgggcg ccatggagac cctcgagcaa ggcgccgaga
     6541 cgctcctgac cagaggaccg ggccggatcg ggcccttctt cgcgccgatg tccctgccga
     6601 acatggcggc tggaatgagc gcgatcttcc tgggcgccaa gggaccgtgc gtcggcaccg
     6661 tgtccgcctg cgcgtcttcc gcgcacgcga tcggtgaggg cgtcgagatg atccggcgcg
     6721 gcgtcgttga cgtcgtctat gcgggtggga ccgaagcgcc ggtgacacga ctgagcgtgg
     6781 ccgggttcgg cgcgatgggc gcgctttcga agcggaacga cgagcctgag cgcgcgagcc
     6841 gcccgttcga tgccgatcgc gacggcttcg tgcttggcga gggagcggcg gtgctcgtgc
     6901 tcgaatcgct tgagcatgcc gagaagcgcg gcgctacgat cctggccgag gtggcaggat
     6961 atgcggccac cgacgacgcg aat
//
LOCUS       DATAOZ010000111        12440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3465486, whole genome shotgun sequence.
ACCESSION   DATAOZ010000111 DATAOZ010000000
VERSION     DATAOZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12440
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3465486"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VHR64_03115"
     CDS             <1..423
                     /locus_tag="VHR64_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX3301844.1"
                     /translation="PAPEGEGVGRAMRLALVDAGLSPADIGYLNAHATSTKIGEKYET
                     QAIKSVFGDGAYSLPVSSTKSMTGHLLGAAGSLEATFCINALRTGCLPPTINYETVDP
                     DCDLDYIPNTAREVRLRAVMNNSLGFGGHNVSVIFISV"
     gene            complement(782..1936)
                     /gene="rpoD"
                     /locus_tag="VHR64_03120"
     CDS             complement(782..1936)
                     /gene="rpoD"
                     /locus_tag="VHR64_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX3301845.1"
                     /translation="MVSSLLDKGRDQGFVLSDDIMAMYPNAEDHVETIDGLYSWLISE
                     GIEVLDRAPAIQPDIDDQVRAEEQAEAEEKALAHEAGIPAVGDSVRLYLHEIGATDLL
                     TMDEEVWLSKRIELGRFAEERLREADLPLAERRQLESIRLDGAAARDHLIQANLRLVV
                     SVAKRYVGRGMSFLDLIQEGNIGLMKATEKFDYKRGFKFSTYATWWIRQAITRAISDQ
                     SRTIRLPVHVGETINRVRKIAHRLQQILEREPSEEEVARALDLSTERVRQILDHSRTP
                     ISLEAPVGSEGDAFLGDFIEDDKLPQPIELASHQLMKEQIAEALGKLSERERKIIELR
                     FGLCDGKYRTLEEVGREFGITRERIRQIEAKALRKLRHPSYSRRLRGYLE"
     gene            2238..2987
                     /locus_tag="VHR64_03125"
     CDS             2238..2987
                     /locus_tag="VHR64_03125"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3301846.1"
                     /translation="MSGHSKWSTIKRQKGATDAKRGQLFTKLAREITVAARSGLPDPE
                     SNGRLRLAVQRARAENMPRDNIERAIERASASGAGDNFEEVTYEAFLPGGIALMVQSQ
                     TDNRNRTVSEVRAVITRGGGNVGADGSVAWMFDNVGQIVVRQGEMSEDDLAMLAIDAG
                     ATEFEPDGETTVVFTEPSDLHKVQETLTRAGLEIDSADLVMKAKDLIAVEPDQAVKVI
                     RVIEKLEDLDDVQNVYTNLEVTDEVFAQVSA"
     gene            2988..3488
                     /gene="ruvC"
                     /locus_tag="VHR64_03130"
     CDS             2988..3488
                     /gene="ruvC"
                     /locus_tag="VHR64_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX3301847.1"
                     /translation="MRILGVDPGTARLGFGIIDAGDGYAAVDFGTIETSSKDVMAHRL
                     LELYDGLTHLIATHAPETMAIEQLFFSRNVTTAIAVGQARGVALLAAAQAGIPVTEYT
                     PAEVKQAISGYGNADKQQVQFMVQLLLELDAVPYPDDAADALAVALCHGQSHAFQDRI
                     STSGYN"
     gene            3492..4286
                     /locus_tag="VHR64_03135"
     CDS             3492..4286
                     /locus_tag="VHR64_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX3301848.1"
                     /translation="MAGTRQRLDDAMVERELAETRARARALVMAGDVLVDGEIVRTAG
                     KPVRPEQEISLREKQRFVSRGGEKLDHALTEFGIDPAGWVCADFGASTGGFTDVLLGR
                     GAERIYAIDVGYGQLHQRIRENERVVVLERTNVRLLESLPEPIDLVVIDVSFISLKLV
                     LPAAVAVVKPGGWCVPLIKPQFEAGRDQIGKGGVVRDPVVHRSVIRDALNAASSLGFV
                     PLDLTQSPLTGPAGNIEFLAALRYREGETVPVETLLERVGLADETV"
     gene            4273..5016
                     /gene="larB"
                     /locus_tag="VHR64_03140"
     CDS             4273..5016
                     /gene="larB"
                     /locus_tag="VHR64_03140"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX3301849.1"
                     /translation="MRPFDDLRGELERAGDELANQPVRSDNGRVSRTGTPEVIYAARK
                     SIEQVLAGIDGLIGNRGRVVVSQIPGDMAMALRDQASSDRSVSFAPGERAAVIADHGS
                     ERPTTGGRVAVISAGTSDIPVAAEAALIASEMGCAVQTTWDVGVAGIHRLVRPLEAMI
                     EWDADVFIVAAGMDGILPTIVSGLVPQPIIGLPISTGYGFGGEGVGALTTMLQSCSPG
                     LSVVNIDNGIGAGITAARIANRAAVARTE"
     gene            complement(5043..5957)
                     /gene="meaB"
                     /locus_tag="VHR64_03145"
     CDS             complement(5043..5957)
                     /gene="meaB"
                     /locus_tag="VHR64_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX3301850.1"
                     /translation="MGLFDRARSGDQAALARLATLIENDRPDAIAALDALGLPERPVH
                     VVGITGPPGAGKSSLINHLLAELRKSGERIAVLLIDPSSQASGGAVLGDRVRMLSWGD
                     PDLFVRSQATRGQEGGLAPSTATLIDLFAQMGFSLVLIETVGVGQDGIDIRAVCDTTI
                     VVQAPHLGDSIQALKAGILEIADIFVVTKSDLPGAQQVVRDLNAMLHLIAQGEPDWSI
                     PVVPVSATEETGFDALAEKLSEHRAWLSCHQAADRRASRLRWEITKRATARVAATSRR
                     LPAEAWKDGESRGDRTRSLLQATLNLDP"
     gene            complement(5960..6385)
                     /locus_tag="VHR64_03150"
     CDS             complement(5960..6385)
                     /locus_tag="VHR64_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX3301851.1"
                     /translation="MSTTDSHPIRVLIAKPGLDGHDRGAKVLVRALRDDGMEVIYTGL
                     FQTPEMIAVAALQEDVQVVGLSILSGAHLSLFPKIVAELKDRGLDHVLLVAGGTIPPR
                     DVQRIKDLGVNEVFGPGSSLTSIIEYIRNNAPQPVAETL"
     gene            complement(6372..7220)
                     /locus_tag="VHR64_03155"
     CDS             complement(6372..7220)
                     /locus_tag="VHR64_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX3301852.1"
                     /translation="MTHTIGVVGSGTMGSGIAQVAAQAGCDVILLDTTLERSRGGLSK
                     IESSLKRLADKGKISVDDATAITGRITPSADFSALANSPLVIEAVFEDPDTKRGVIEA
                     VSAVTDPSSIFATNTSSISITKLASYHPHPDKVAGMHFFNPVPLMPLVELIRAEQSSP
                     ETVEYVREKAESFGKTVVEVADVPGFVANRVLMPMINEAILCLEGGVASAEAIDQTLK
                     LGANHPMGPLALADLIGLDVVLNIIEVLHRDLGEDKYRPAILLKRMVAAGKLGRKSGE
                     GFYVYD"
     gene            complement(7224..7814)
                     /gene="pth"
                     /locus_tag="VHR64_03160"
     CDS             complement(7224..7814)
                     /gene="pth"
                     /locus_tag="VHR64_03160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX3301853.1"
                     /translation="MKIIVGLGNPGREYERTRHNIGFDVTDELVRRLSGVSQRNRFRA
                     IVREGVLGGTKFIVAQPQMYMNLSGVPVREIVNWYKTDLDDVLIVYDDMDLPLGQLRL
                     RADGSAGGHNGMKSIIQELGTTEFARLRIGIGRGGGSSTAHVLSRFRPEEASAMSQTI
                     EDAVDAILLWSERGAIEAMNIVNRPAAETEPSPGVA"
     gene            7929..8558
                     /locus_tag="VHR64_03165"
     CDS             7929..8558
                     /locus_tag="VHR64_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX3301854.1"
                     /translation="MNFIGIGYGEILIIIVAALIIFGPDKLPEVAGQAARWLRDARKM
                     MTEMTGEFERSAGMKEFKEAIEGEIKGVKKELEDAGLSVKNDLSKAAGSANAAVSGAA
                     SAARGTKPTGAAKTTAAATGTKPATSTTNKPAGKPKASKQDPYAGLVQLTEVTPRKQR
                     AAVVVSETVANGNGSGNGSTEPIRETSEAVMRARERRLAAGYNRRVTTP"
     gene            8566..9723
                     /gene="tgt"
                     /locus_tag="VHR64_03170"
     CDS             8566..9723
                     /gene="tgt"
                     /locus_tag="VHR64_03170"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX3301855.1"
                     /translation="MTEFRYELIGTDPAPRARRGRVHTRRGVIETPVFMPVGTQATVK
                     TLEPHEVADTGAQIVLANTYHLMLRPGVEIIRAAGGVHAFSRWQGPILTDSGGFQIFS
                     LASQVKVREEGASFQSHIDGSHWHLTPERAIELQAGYGSDIMMALDHVVGYPAKERAV
                     ADAMQRTHRWLDRCIARTELGDLRPEQGVLFGIVQGGMYPELRSESAQQVASRNVAGV
                     AIGGLSVGEPKDVMAEFLDITMLHLPEHKPRYLMGVGSPEDLWNGVAQGVDMFDCVLP
                     TRLARNAALFTLDGRINIRKRHYRDVFRPVDEDCDCHTCQNYTAAYLHHLYRADEILG
                     LKLGTIHNVRFLVRQMQIMRAAIMSGTFAAEHRAFLDRYKVVGQTVTQEAV"
     gene            9728..10171
                     /gene="acpS"
                     /locus_tag="VHR64_03175"
     CDS             9728..10171
                     /gene="acpS"
                     /locus_tag="VHR64_03175"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX3301856.1"
                     /translation="MYVPEFDADKCGRVVVGIDLIEIPRIQRALDDFGERFLNRVYTQ
                     RERDRYRTRISELAARFAAKEAISKALGTGIRGIKWREMEVVSNRRGKPIVVLHGTAA
                     ERAELLGITDFDISLTHSRTDAMAFVVGFRAEDDPPAVDMETGEQ"
     gene            10168..11154
                     /locus_tag="VHR64_03180"
     CDS             10168..11154
                     /locus_tag="VHR64_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HEX3301857.1"
                     /translation="MTDTGVTIREFSRDDAIRVLPRRSPSAHKWGVGGLLIIAGSPGY
                     VGAAALCAMAAGRAGAGIVNLAVHRALVSSITPVVPEVGFSILPEGDLSSSGKRVLDA
                     LEKKAGKCHAFVVGPGLGDDEYALDLMTTLLGKTERASLSSLGFGTPRPQPAEATAEQ
                     HSLLAYNRPILIDADGLNALAKIEDWWSHVPEHLLILTPHVGEFSRLMDMSPEDVLAS
                     PERAAVAAANRFRQVVLLKGAPTIVTDGRVVFRAADSPPSLATGGSGDVLSGTIGALL
                     AQGLSLVDAANLGVFAGARAARRLELELGTLGLVASDLPHAIAIELAALERE"
     gene            11151..12353
                     /gene="alr"
                     /locus_tag="VHR64_03185"
     CDS             11151..12353
                     /gene="alr"
                     /locus_tag="VHR64_03185"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX3301858.1"
                     /translation="MTPITDPTLAAVYEQSGRGTRAIVDLDAIAGNVRALRDLLPAST
                     SLMAVVKGDGYGHGAVMVARCAISSGATVLGVATIGEAAELREYQIATPILLLGPCDV
                     SEVRRALELGVEITVAGPELLDAIDAVAAEMGRAARVHLKIDSGMHRYGASPADALAM
                     ADRIAQLEHVDLVGVSTHYAAADETKHAASAQQAAVFEQVAAQVRVRVGHPFALHSAN
                     SAATLRGMAAGTDIARCGIALYGLAPSAQVNLAPGMRPALSLISRLTRVHLGRGGDGL
                     GYGHTYVCTTDERFGLIPLGYADGYRRALSNRAWVCIGGRRCPVRGTVSMDQTVAGDL
                     AETALTGDLVGVAGPLSRGPGFDELAELCGTIPYEMLTGLSRRVPRYYVTNGRVVATL
                     IENSLESV"
BASE COUNT         2196 a         3909 c         4127 g         2208 t
ORIGIN      
        1 ccggctcccg agggcgaagg cgtcggccgg gcgatgcggt tggcgctggt cgacgcggga
       61 ctctcgccgg cggacatcgg ctacctgaat gcgcacgcga cctcgaccaa aatcggcgag
      121 aagtacgaga cgcaggcgat caagtccgtt ttcggtgatg gtgcgtactc gttgccggtc
      181 tcctcgacca agtcgatgac cggccacttg ctcggcgcgg ccggttcgct ggaggcgacc
      241 ttctgcatca acgcgctgcg gaccgggtgc ctgccgccga cgatcaatta cgaaacggtc
      301 gatcccgact gcgacctcga ttacatcccg aacacggctc gcgaggtcag actccgcgcc
      361 gtcatgaaca acagcctcgg cttcggcggc cacaacgtct cagtcatctt tatctcggtg
      421 taaatcagct caccaaaatg tggtgcgagt gacacggtag atggagtccc tataacgccc
      481 gctcggcgcc gccccggact gaaggccggg gctgaggaag ataaggcggc taaagccgac
      541 ttccagttag tccactttag tggacttatc gtcgcttagc ccgggccttt agccccgggc
      601 ggcaccggga agcccgttgg aggatacaaa tccggtcgac gactcctcta tcatgcatgc
      661 cgctccgcgt ctttgtgggg cacgaaggca cccgcgccac attggacacc gatttggaga
      721 cgaaaagtgg gcgggaagat tcccgccccg tttctgaagt ctgaagtccg aagtctgccg
      781 actattccag gtatccgcgc aggcgccggc tgtaggaggg gtgacggagc ttgcggaggg
      841 ccttggcctc gatctggcgg atccgctcgc gcgtgatccc gaactcgcgg ccgacttcct
      901 cgagcgtgcg gtacttgcca tcgcagaggc cgaagcggag ctcgatgatc ttgcgttcgc
      961 gctcggagag cttgcccagc gcctcggcaa tctgctcctt catgagctga tgcgaggcga
     1021 gctcgattgg ctgcggcagc ttgtcatcct cgatgaagtc gcccaggaag gcatcgcctt
     1081 cgctcccgac cggcgcctcg agcgagatcg gcgtgcggga gtggtccaga atttggcgga
     1141 cccgctcggt cgagagatca agcgcgcgtg cgacctcttc ctcgctcggc tcgcgctcga
     1201 ggatctgttg cagcctgtgc gcgatcttgc ggacacggtt gatcgtctcg ccgacgtgga
     1261 caggcagccg gatcgtgcgg ctctgatccg agatggcgcg ggtgatcgcc tggcgaatcc
     1321 accaggttgc gtaggtgctg aacttgaagc cgcgcttgta atcgaacttc tcggtggcct
     1381 tcatcaggcc gatattgcct tcctggatca ggtccaggaa tgacatgccg cggccgacat
     1441 agcgtttggc gaccgagacc acgaggcgaa ggttggcctg gatgaggtga tcgcgggccg
     1501 cggcaccgtc gagccggatc gactcgagct ggcgtcgctc ggcgagcggc aggtcggcct
     1561 cgcgcaggcg ctcctctgca aagcgtccga gctcgatccg cttggagagc cagacctctt
     1621 cgtccatggt cagaaggtcg gtcgcgccaa tctcatgcag gtagaggcgg actgagtcgc
     1681 cgactgccgg gatgccggcc tcatgggcga gcgccttttc ctcggcctcc gcctgctcct
     1741 cggcccggac ctgatcatcg atgtcaggct gaatggcggg cgcccgatcg aggacttcga
     1801 tcccttcgga gatcagccaa ctgtagaggc cgtcgatcgt ctctacgtgg tcttcggcat
     1861 tcggatacat cgccatgatg tcatccgaaa ggacgaatcc ctgatcccgg cccttgtcga
     1921 gcaaactgga aaccatcgtt gcgtgcgccc cgtcgattaa gggagtccgt tctcccgcca
     1981 gttcaacgcc gtgtcctgcg tccttgaagg taagattcgt ccgcgcagat catgtcgata
     2041 actgcatccg gtgatatctc gctaccccaa attgtttaca gtctacgatg gcgtgctaca
     2101 agtttcaaag gtctggtgaa ttggttacat actactggta cggaccagtt cctgacaacc
     2161 cctcaggatc ggtgcgcgat ccggccggtg atatactcgg agaccgtata acccgctttg
     2221 aaccgaaggt ttctccgatg tctggacact ccaaatggtc gacgatcaag cgacaaaagg
     2281 gcgccacgga cgccaagcgc ggtcagctct tcaccaagct ggcgcgcgaa atcacagtcg
     2341 ccgcgcggtc tggactgcct gaccccgagt ccaacggtcg gctccggctc gcggtccagc
     2401 gagcaagggc cgagaacatg ccccgcgaca acatcgagcg ggcaattgag cgggcatccg
     2461 ccagcggagc gggcgacaac ttcgaagagg tgacctacga ggctttcctg cccggcggca
     2521 ttgccctgat ggtccagtcg cagaccgata accgcaaccg caccgtcagc gaggttcgcg
     2581 ccgtgatcac ccgcggcggc ggtaatgtcg gcgcggatgg cagcgtggcc tggatgttcg
     2641 acaacgtcgg gcagatcgtt gtcaggcagg gcgagatgtc ggaggacgat ctcgcgatgc
     2701 tggcgatcga tgccggcgcg accgagttcg agccggatgg cgagaccacc gtcgtcttca
     2761 ccgagcccag cgatctgcac aaggtccagg agaccctgac ccgggcgggg ctggagatcg
     2821 attccgctga tttggtcatg aaggccaagg atctgatcgc agtcgagccg gaccaggcag
     2881 tcaaggtgat tcgcgtgatc gagaagcttg aggacctgga cgacgtccag aacgtctaca
     2941 ccaacctcga ggtgaccgac gaggtgtttg cccaggtcag cgcctaggtg cggattctcg
     3001 gggtcgatcc cggcacggcg cggctcggct tcggcatcat cgatgcaggc gatggctacg
     3061 cggcggtcga tttcggcacg atcgagactt cctcaaagga cgtgatggcg catcgcctgc
     3121 tcgagctcta cgacggcctg acgcacttga tcgcgaccca cgcgcccgaa acgatggcaa
     3181 tcgaacagct cttcttttcg cgcaacgtca caacggccat cgccgtcggt caggcgcggg
     3241 gcgttgccct gctcgccgcg gcacaggccg ggattccggt cacggaatac acgccggcgg
     3301 aggtcaagca ggcaatctcc ggatatggca atgccgacaa gcagcaggtc cagttcatgg
     3361 tccagctgct gctcgagctg gatgcggtcc catacccgga cgacgcggcc gacgcgttgg
     3421 ccgtcgccct atgtcacggc cagagtcacg ccttccagga tcgcatctca acgagcggat
     3481 acaactaggg gatggccgga acgcggcaac gtttggacga cgccatggtc gaacgcgagc
     3541 ttgcagaaac ccgggcgcgg gctcgggcgc tggtaatggc cggggacgtc ctggttgatg
     3601 gtgagatcgt gcggacagcc ggcaagcccg tccggcccga gcaggagatc tcgctgcgag
     3661 agaagcagcg gttcgtgagc cgcggtggcg agaagctcga ccacgcgctg accgagtttg
     3721 gcatcgatcc ggccggctgg gtctgcgccg atttcggggc cagtaccggc ggattcaccg
     3781 atgtcctgct tggtcgcggt gcggaacgga tctatgcaat cgatgttggc tacgggcagc
     3841 ttcatcaacg tatccgcgag aatgaacgtg tcgttgtttt ggagcggacc aatgtccggc
     3901 tgctcgagtc gctgccggag ccgatcgatc tggtcgtgat cgacgtctcg ttcatctcac
     3961 tgaagctggt cctgcctgcc gctgtggctg tggtgaaacc cggcggctgg tgtgtcccgt
     4021 tgatcaagcc gcagtttgag gcaggtcgcg atcagatcgg caagggcggc gtggtccgcg
     4081 acccggttgt tcaccggtcg gtgatccggg acgcgctgaa tgcagccagc agtctcggct
     4141 tcgtgccgct cgatctgaca caatcgccct tgaccgggcc ggccgggaat atcgagttcc
     4201 tggccgcgtt gagatatcga gaaggcgaga cggtccccgt cgagacgctg ctcgagcgcg
     4261 tagggttggc agatgagacc gtttgatgat ttgcgcggcg aacttgagcg cgctggggac
     4321 gagctggcca accagccggt acggtccgac aacggccggg tcagccggac aggaacgcct
     4381 gaagtcatct atgcagcgcg caaatcaatt gagcaggtat tggccgggat cgatggactg
     4441 attggcaatc gcggtcgggt cgtcgtgtct cagatcccag gtgacatggc gatggcgctc
     4501 cgggatcagg cttcgtcaga caggtcggtg agcttcgcac cgggcgagcg cgcggctgtg
     4561 atcgctgacc atggctccga gcggccgacc acgggtggca gggtcgcggt aatcagcgcc
     4621 gggacatccg acattccggt agccgccgag gccgcgctga tcgcgtcgga gatgggctgc
     4681 gcggtccaga cgacgtggga cgtcggcgtg gccgggattc accggctggt gcgtccgctc
     4741 gaggccatga tcgagtggga tgccgatgtg ttcatcgtcg cggccgggat ggacggaatc
     4801 ctgccgacga tcgtctccgg gctggtcccg cagccgatca tcggattgcc gatctcgacc
     4861 ggctacggct tcggtggtga gggtgtgggc gcgctgacca cgatgctcca gagctgctcg
     4921 ccggggttgt cggtcgtcaa catcgacaac gggatcggcg ctggcattac tgcggcccgg
     4981 atcgcgaatc gggcggcggt ggcgaggacc gagtgaccgg atggtcgcat tgccatgcga
     5041 cgctacgggt ccaggttgag cgtcgcttgc agcagcgagc gggttcggtc accgcgcgat
     5101 tcgccatctt tccacgcctc ggcgggcaag cggcgcgaag tcgcggcgac gcgtgccgtc
     5161 gctcgcttgg tgatctccca gcgcagtcga gaggcgcgcc ggtcggcagc ctggtgacat
     5221 gaaagccacg cgcggtgctc actgagcttc tccgcaaggg cgtcgaatcc ggtctcctcg
     5281 gtcgccgaga ccggcacgac cgggatcgac caatcgggct cgccctgcgc gatcaggtgc
     5341 agcatggcgt tgagatcgcg gacgacctgt tgcgcgccgg gcaaatctga cttggtgacc
     5401 acgaagatgt cggcgatctc gaggatgccc gccttgagcg cctgaatgct gtcgccgagg
     5461 tgcggagcct ggaccacgat cgttgtgtcg cagacagccc ggatatcgat gccgtcctgg
     5521 ccgacaccca ccgtctcaat cagcacgagg ctgaaaccca tctgcgcgaa gaggtcgatc
     5581 agcgtcgcgg tcgagggcgc caatccgcct tcctgcccgc gcgtggcctg gctgcgcacg
     5641 aagaggtctg gatcgcccca tgagagcatg cgcacccggt cgccgaggac agcgccgccg
     5701 ctggcctggc tcgatggatc gatcagcagc acggcaattc gctcgccgct cttgcgtagc
     5761 tcggccagca agtggttgat cagcgagctc ttgccggcgc cgggtggacc ggtgatcccg
     5821 accacgtgga ccggccgctc gggcaggccg agggcatcga gcgcggcaat tgcatcgggc
     5881 cggtcattct cgatcagggt cgccaagcgg gcgagtgccg cctggtcacc cgaccgtgcg
     5941 cgatcgaaca gtcccatcgc taaagggtct cggcgaccgg ctgtggggcg ttgttgcgga
     6001 tgtactcgat gatcgacgtc agcgaagaac ccggcccgaa gacctcgttg acacccaaat
     6061 ccttgatccg ctgcacgtcc cgtggcggga tcgtgccgcc cgccaccagc agaacatgat
     6121 ccaggccgcg atccttcagc tccgcgacga tcttcgggaa gagcgagagg tgcgcgccgg
     6181 agaggatgct cagacccacc acctggacgt cttcctggag cgcagccacg gcgatcatct
     6241 cgggcgtctg gaacaggccg gtatagatca cctccatacc gtcatcgcgg agcgcgcgga
     6301 cgaggacctt ggcgccgcga tcgtgcccgt cgaggcccgg cttggcgatc aggacgcgga
     6361 tagggtggct gtcagtcgta gacataaaat ccctcgcccg acttgcgtcc gagttttccg
     6421 gcggcaacca tccgcttgag cagaattgcc ggacggtact tgtcttcgcc gagatcgcga
     6481 tgcagcacct cgatgatgtt caggacgacg tccagtccga tcaggtcggc cagggcaagt
     6541 gggcccatcg ggtggttcgc gccgagcttg agtgtctggt cgatcgcctc ggccgaagcg
     6601 acgccgccct cgaggcagag aatcgcctcg ttgatcatcg gcatcaggac gcggttggcg
     6661 acgaagccgg gcacatccgc gacctcgaca acggtcttgc cgaatgactc cgccttctcg
     6721 cggacatatt cgacggtctc cgggctggat tgctcggccc ggatcagctc gaccagcggc
     6781 atcagcggaa cggggttgaa gaagtgcatc ccggcaacct tgtccggatg cgggtggtag
     6841 gacgccagct tcgtgatcga gatcgacgac gtgttggtcg cgaagatcga ggatgggtcc
     6901 gtgaccgcgc tgaccgcttc aatcacgccc cgcttggtat cgggatcctc gaagaccgcc
     6961 tcgatgacca gcggcgaatt cgcgagcgcg ctgaaatcag ccgacggtgt gattcgaccg
     7021 gtgatcgcgg tcgcgtcgtc gacagagatc ttgcccttgt cggccagccg cttgagcgac
     7081 gactcgatct tgctcaggcc gccccgcgac cgctctaggg tcgtatcgag caggatgaca
     7141 tcgcagccgg cctgcgccgc cacctgggcg atcccgctgc ccatggttcc gctgccgacg
     7201 acgccgatgg tgtgggtcat gtctcaggct actcccggac tcggctccgt ctcggcagcc
     7261 ggtcgattga cgatgttcat ggcctcgatc gcgccacgct cggaccagag caggatcgcg
     7321 tcgaccgcat cctcgatcgt ctggctcatc gcgctggcct cttccggccg aaaccgcgag
     7381 aggacatggg ccgtgctgct tccgccgcca cgaccgatgc cgatgcgcag gcgcgcgaac
     7441 tcggtcgtgc ccagctcctg gatgatcgac ttcatcccgt tgtgcccgcc agcagatccg
     7501 tcggctcgaa gtcggagctg tccgaggggc aggtccatgt cgtcgtagac gatcagcacg
     7561 tcgtcgagat cagtcttgta ccagttgacg atctcgcgca ccggcacgcc gctcaggttc
     7621 atgtacatct gcggctgcgc gacgatgaac ttggtgccgc ccaaaacgcc ttcgcggacg
     7681 atcgcgcgga aacgatttcg ctgcgagaca ccactcagcc ggcggaccag ctcatcggtg
     7741 acatcgaagc cgatattgtg ccgcgttcgc tcgtattcgc ggcccggatt tcccaatccg
     7801 acgatgatct tcacgtgccc tcatttactc tgccacggcg aaggaccgcc gtatgctcaa
     7861 tgctatactc caaaccggtt cctcggcaga gaatcagact gtagccgccg agcacgcagg
     7921 gcacctcgat gaacttcatt ggtatcggat acggcgagat tctgatcatc atcgtcgccg
     7981 cgctgatcat ctttggtcca gacaagctcc ccgaagtcgc cggccaggcc gcgcgctggc
     8041 ttcgcgacgc ccgcaagatg atgacggaga tgaccggcga gttcgagcga agcgccggaa
     8101 tgaaggaatt caaggaagcg atcgagggcg agatcaaggg cgtcaagaaa gagctcgagg
     8161 atgccggcct ttcggtcaag aacgacctca gcaaggccgc cggaagtgcc aacgcggcgg
     8221 tatcgggcgc tgcgtccgcc gccagaggaa ccaagccgac cggcgccgcc aagaccaccg
     8281 ctgcggcaac tggcaccaag ccggcgacct cgacaacgaa caagccggcc ggaaagccga
     8341 aagcctccaa gcaggaccca tacgcgggcc tggtccaatt gaccgaagtc accccgagga
     8401 agcaacgcgc ggctgtcgtc gtttccgaaa cggtcgccaa cggaaatggg tccggcaatg
     8461 ggtcgacgga gccgattcgc gagaccagcg aagcggtgat gcgcgcccgc gagcgacgcc
     8521 tggctgccgg atacaaccgg cgggtcacca cgccatagct ggatcatgac cgaattccgg
     8581 tacgagctga tcgggaccga cccggcccca cgcgcccgtc gggggcgagt tcatacccgg
     8641 cgtggggtca tcgaaactcc ggtcttcatg ccagtcggga cccaggccac tgtcaagaca
     8701 ctcgagccgc acgaagtcgc cgacaccggc gcgcagatcg tgctcgcaaa cacgtaccac
     8761 ctgatgcttc gaccgggcgt cgagatcatt cgcgcggccg gtggcgtgca cgccttctcc
     8821 cggtggcagg gcccgatcct gaccgacagc ggcggattcc agatcttcag cctggcatcg
     8881 caagtcaagg tgcgggaaga gggcgcgtca tttcagtcgc acatcgacgg atcgcactgg
     8941 catttgaccc ctgagcgcgc gatcgagctc caggcagggt acggctccga catcatgatg
     9001 gcgctcgacc atgtggtcgg atatccggcc aaagagcggg ccgtcgccga tgccatgcag
     9061 cgcacccatc gctggttgga ccggtgcatc gcgcggaccg aactcggcga cctgcgaccg
     9121 gagcagggcg tgctcttcgg catcgtccag ggagggatgt atcccgagct gcgctccgag
     9181 agcgcgcagc aggtcgcgtc ccggaatgtg gccggtgtcg cgatcggcgg gctgagcgtc
     9241 ggcgaaccga aggatgtgat ggccgagttt ctcgatatca cgatgctgca cctgcccgag
     9301 cacaagccgc gctatttgat gggcgtcggt tctccggagg atctctggaa cggcgtcgcc
     9361 cagggcgtcg acatgttcga ctgcgtcctc ccgactcgcc tcgcccgcaa tgcggcgttg
     9421 ttcacgctgg acggtcggat caatatccgc aagcgccact atcgcgatgt cttccggccg
     9481 gtcgacgaag attgcgactg ccacacctgc cagaactaca ccgcggcgta cctgcatcac
     9541 ctctatcggg cggacgaaat attggggctg aagctcggca cgatccacaa tgtccggttc
     9601 ctcgtgcggc aaatgcagat catgcgcgcg gcgatcatgt ccggcacgtt tgcggctgag
     9661 catcgggcat tcctggatcg gtacaaggtg gttggccaga cggtgacgca ggaggcggtc
     9721 tgattccatg tacgtcccgg aattcgatgc ggacaaatgc ggtcgggtcg tcgtcgggat
     9781 cgatctgatc gagatcccgc gcattcagcg cgcgctcgac gacttcggcg agcgtttcct
     9841 gaaccgggtc tacactcagc gcgagcggga tcgttaccgc acccgcatct cggagctggc
     9901 cgcgcgattc gccgccaagg aagcaatctc gaaggcgctg ggcacgggta tccgcggcat
     9961 caagtggcgc gagatggagg tcgtctcgaa tcggcgcggc aagccgatcg tcgtgcttca
    10021 tggaacagcg gcagagaggg ccgagctcct cggcatcacc gacttcgaca tctcgttgac
    10081 gcactcgcgg accgatgcga tggcgttcgt tgttgggttt cgggcggagg acgacccgcc
    10141 cgcagttgac atggaaacag gtgagcaatg acagacacag gcgtgacgat ccgcgagttc
    10201 agtcgagacg atgcgatccg ggtgctgccc cggcgctctc ccagcgcgca caaatggggc
    10261 gtcggcgggc tcctgatcat tgcgggctcg ccgggctacg tcggcgccgc cgccctctgc
    10321 gcgatggccg cgggtcgggc gggcgccggc atcgtcaacc tcgccgtgca tcgcgcccta
    10381 gtctcatcga ttacgccggt ggtgcccgag gtggggttct cgattctccc cgagggcgac
    10441 ttgagctcca gtgggaaacg cgttctggat gcgctcgaga agaaggccgg gaagtgccac
    10501 gccttcgtcg tcggccccgg gctgggcgac gatgaatacg cgctcgacct gatgacgacg
    10561 ctgctcggga agacagagcg cgcgtcgctc tcgtccctgg gattcggcac accgcgaccg
    10621 cagccggccg aggccactgc cgagcagcac tcgctcctgg cctacaaccg accgatcctc
    10681 atcgatgccg acgggttgaa cgcgctggcc aagatcgaag actggtggtc gcacgttccc
    10741 gaacatctac tcatactgac gccgcacgtg ggcgaattca gccggctgat ggatatgagt
    10801 cccgaggacg tcctggccag tccggaacga gcggcagttg ccgcggccaa ccggttccgg
    10861 caagtcgttc tgctgaaagg cgcaccgacg atcgtgaccg acggccgggt cgtattccgc
    10921 gcggctgact cgcctccgtc gcttgccacc ggagggagcg gggatgtgct gtccggcacg
    10981 atcggtgcgt tgcttgccca gggcttgtcg cttgtcgatg cggcgaatct gggagtcttc
    11041 gcgggcgcgc gcgcagcccg gcgactggag ctggaattgg gcacgcttgg gctcgtggcc
    11101 agcgatcttc cgcacgcaat cgcaatcgag ctggccgcgc tggagcggga atgactccga
    11161 tcactgaccc gactctggcc gctgtctacg aacagtcggg tcgcggtacc cgcgccatcg
    11221 ttgacctcga cgcgatcgca gggaacgtca gagcactcag ggacctgctg ccggcgtcaa
    11281 cctcgctgat ggcggtggtc aagggcgacg gatacggcca tggcgccgtg atggtggccc
    11341 gctgcgcgat ctcttcaggt gcgaccgtgc ttggggttgc cacgattggt gaggcggccg
    11401 agctccggga gtatcagatc gccacgccga tcctgctcct ggggccgtgc gacgtgtctg
    11461 aggtccgccg cgcactcgag ctcggtgtgg agatcaccgt ggccggcccg gagctgctcg
    11521 acgccattga cgcggtagct gccgagatgg gcagggcggc tcgggttcat ctcaagatcg
    11581 attccggcat gcaccggtac ggcgcatcgc ccgccgacgc actcgcgatg gccgatcgaa
    11641 ttgcgcaact cgagcatgtg gacctggtcg gcgtctcgac ccactacgcg gccgcggatg
    11701 agaccaagca cgctgctagc gcgcagcagg cggcggtctt cgaacaggtc gcggcgcagg
    11761 tccgggtcag ggtcgggcac ccgttcgccc tgcattcggc gaacagtgcc gcgacgctgc
    11821 gcgggatggc tgccgggaca gatatcgctc gctgcggaat tgccctctat ggccttgccc
    11881 ccagcgcgca ggtcaacctc gcgccgggga tgcgcccggc cttgagcctg atctcgcgcc
    11941 tcacaagggt ccacctgggg cgaggcggcg atggcctcgg ctacggccac acctacgtct
    12001 gtacgaccga cgaacggttc ggcttgatcc cgctcggcta tgccgatgga tatcgacggg
    12061 cgctctccaa ccgggcgtgg gtctgcattg gcggccggcg atgcccggtc cgcggcaccg
    12121 tcagcatgga ccagacggtt gccggcgatc tggcggagac cgccctgacc ggcgatctcg
    12181 tcggcgtggc cggtccgctg tccagaggcc cgggtttcga cgagctggcc gagctctgcg
    12241 gaacgattcc gtacgaaatg ttgacgggcc tgagccggag ggttccgcgc tattacgtga
    12301 cgaacggtcg cgtcgtcgcg acgctgatcg agaacagcct cgagtcggta tgagcaaacg
    12361 aaagcggccg ggaaaaattc cccggccgca gcgtgtgtct gtttcctaga agacggagct
    12421 ggcctctacg tccaggtccg
//
LOCUS       DATAOZ010000112         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8990059, whole genome shotgun sequence.
ACCESSION   DATAOZ010000112 DATAOZ010000000
VERSION     DATAOZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8990059"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..937)
                     /gene="map"
                     /locus_tag="VHR64_03190"
     CDS             complement(188..937)
                     /gene="map"
                     /locus_tag="VHR64_03190"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX3301859.1"
                     /translation="MAITIKNEREIELMRQAGHIVGLAHARIQSAIQPGVTTRDLDDI
                     AREVIEENGATATFYGHLGFTGRICASVNEEIVHGIPGKRTLRDGDIISVDIGATYNG
                     FVGDSAWTYPVGTIDDESKRLLEVTEQSLYLGIEQAVAGNRLGDIGYAIEQFILASGF
                     GMVREYGGHGVGRSLHEDPFVTHVGKVGTGPEIRPGMVFTIEPMLNEGGPETRLGEDG
                     WVVSTADGKRSAQFEHTLAITDNGPEILTTL"
     gene            complement(945..1616)
                     /locus_tag="VHR64_03195"
     CDS             complement(945..1616)
                     /locus_tag="VHR64_03195"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX3301860.1"
                     /translation="MNIIVIGPQGSGKGTQADLLGPKIGAIKLSTGDLFRAHIAQETE
                     LGRKVKEILASGNLVSDDITLAMVDGRLDEIAADGIGSVIFDGFPRTAAQAEGLDGLL
                     AERNQKVDRVIELSVPETILIERMSGRRVCANCGTNYNLAFQSTQLEGICDKCGGELI
                     QRPDDMPDAIRKRLELYNDLTKPLLAFYGNRGLVTTIDGNQHVESVQQAIVDALAAAP
                     VNSET"
     gene            complement(1685..>2284)
                     /locus_tag="VHR64_03200"
     CDS             complement(1685..>2284)
                     /locus_tag="VHR64_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HEX3301861.1"
                     /translation="GQRRIPVHYAKRIRGNKQYGGNTTFIPLKVNSAGMIPLIFAVSI
                     MVMPGLIANFLASSNREWVRSVGGDLQRFFSPSNVWYQVVYFLLVVAFTYFYTMVVFY
                     QQKIPETLQRQGAFIPGIRPGRNTAVYLQQVLQRITLVGALFLGLVAIMPYIASQITG
                     IQTLLISATSILIVVGVAIDTMRQLEAQLMMRNYEGFIQ"
BASE COUNT          443 a          695 c          684 g          462 t
ORIGIN      
        1 gaatgccgcg gctagggacc tcactgcgtt cggccacgag gaccgccttt gggccgctgc
       61 ccctccgcgc gactgtcctg cgcgggctag ccagactatg ccgctagcca tccctttgca
      121 ttactcgccc ggtccctccc cttctaaggg agggtggccg aaggccggga gaggtccgcc
      181 cgcgcaccta cagtgtcgtc agaatctcgg ggccgttgtc ggtgatggcg agggtatgct
      241 cgaactgcgc cgagcgctta ccgtcggcgg tgctgacgac ccagccatcc tcgcccagcc
      301 tggtctcagg gccgccttcg ttgagcatcg gctcgatggt gaagaccatg ccggggcgaa
      361 tctccgggcc ggtgcctact ttgccaacgt gggtgacgaa cgggtcttcg tgtagcgagc
      421 gcccgacgcc atggccgcca tattcgcgca ccatgccgaa cccgctggcc agaatgaact
      481 gctcgatggc atacccgatg tcacccagcc gattgccggc aaccgcctgc tcgataccga
      541 gataaagcga ctgctcggtc acttccagca agcgttttga ctcgtcatcg atggttccaa
      601 cgggataggt ccaggcggaa tcgccaacga acccgttgta cgtggcgccg atatccaccg
      661 agatgatgtc gccgtcacgc agcgtgcgct tgccggggat gccatgcacg atctcttcgt
      721 tcaccgaagc gcaaatccga ccggtgaagc cgagatgacc gtagaacgtg gcggttgcgc
      781 cattctcctc gatgacctcg cgcgcgatgt catcgagatc cctggtggtc accccgggct
      841 ggatcgcaga ttgaatccgc gcgtgggcca aaccgacgat atgcccggct tgccgcataa
      901 gctcgatctc gcgctcattt ttgatcgtga tcgccatcga gggatcaggt ctccgaattg
      961 accggcgccg ccgcgagggc gtcgacgatc gcctgttgaa cagattcgac atgttgattc
     1021 ccgtcgatgg tggtgaccag cccacggttt ccgtaaaagg ccagaagtgg tttggtcaaa
     1081 tcgttataga gctcgagccg cttgcgaatg gcgtccggca tatcgtccgg tcgctgaatc
     1141 aattcgccgc cgcacttgtc gcaaatccct tcaagctggg tcgactggaa tgccaggttg
     1201 tagttcgtcc cacaatttgc gcagacgcgg cgaccgctca tgcgctcgat caggatcgtt
     1261 tccgggaccg acagctcgat tacgcgatcg actttctgat ttcgctccgc gagaaggcca
     1321 tccagccctt ccgcttgtgc ggcggtgcgg ggaaagccat cgaatatgac actgccgatg
     1381 ccatcggcgg cgatttcatc cagccggcca tcgaccatcg ccagggtgat gtcgtccgaa
     1441 accagattcc cggaggccag gatctccttg accttgcggc cgagttccgt ttcctgggcg
     1501 atgtgggctc gaaacaggtc gccggtagaa agtttgatcg ccccgatttt ggggccgagc
     1561 agatcggcct gggttccttt gcccgagccc tgggggccga tcacgatgat gttcatcagt
     1621 aaatccgtgt cgtgtcaggt cgattgggca gccggactcg caggtccggc tgcccgaaat
     1681 gaacctattg gatgaaacct tcgtagttgc gcatcatcag ttgcgcttcc agctgccgca
     1741 tggtgtcgat cgccacgccg accacgatca ggatcgaagt cgcgctgatc agcaaggtct
     1801 gaatgcctgt gatctgcgat gcgatgtaag gcatgatcgc caccaaaccg aggaagagcg
     1861 caccgaccag ggtaatccgc tgcaggacct gctgcagata gaccgcggtg ttgcgtcccg
     1921 ggcgaatgcc cggaatgaag gcgccctgtc gctgcaatgt ttccgggatc ttctgttgat
     1981 agaagacgac catcgtatag aagtacgtga acgccaccac cagcaggaaa tagaccacct
     2041 ggtaccaaac attcgacgga ctgaagaagc gttgtaagtc accgccaacc gaacgcaccc
     2101 actcgcggtt cgagctcgcc aggaaattgg cgatcagtcc gggcatcacc atgatgctga
     2161 ccgcgaagat gagcgggatc atgccggcgg aattgacctt caacggaatg aaggtggtat
     2221 tgccaccgta ttgcttgttg ccgcggatgc gcttcgcgta atgcaccggg atgcgtcgct
     2281 gacc
//
LOCUS       DATAOZ010000113         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7938675, whole genome shotgun sequence.
ACCESSION   DATAOZ010000113 DATAOZ010000000
VERSION     DATAOZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7938675"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VHR64_03205"
     CDS             <1..1390
                     /locus_tag="VHR64_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3301862.1"
                     /translation="GDVSRLARNIDGRVMLPGNEGYDSARALWNAMVDRRPAVVVRCV
                     SSADVVAAIDFARRNDLELGIRCGGHSVLGISVPDGGLLIDLSGLRAVRVDADARRAW
                     VQGGALLGDLDKATQAFGLATTAGNVSHTGVGGLTLGGGMGWLARRFGLACDNVASYT
                     LVTADGETLRVSDSQHKDLFWGLRGGGGNFGVVTEFEFRLHEVGTSAVLANRTFDLAE
                     APSAMRHWRDLLDDDVPPEATPLAWVGRMPDSPEVPGELCNRTVAILGFVWVGDPENG
                     RRWLMPRLTSLGVPLAEQVEEVTYLGLQSTDDEWHRPGSRRRYYKGHYLTELSDVAID
                     AFLSQGEPDPEADRPLFLNGSFQGYGGAIAATSNDDSAFSHRNTLVEFVAATAWTDPA
                     EDTQRMEAARRFGAAMEPFASGVYVNVLGDEGELGLRRAYPAEKLDRLAALKRRYDPD
                     NIFHLNQNIRPS"
BASE COUNT          258 a          452 c          524 g          274 t
ORIGIN      
        1 gggcgacgtg agccgcctgg cgcgcaatat cgacgggcga gtcatgctgc ccggcaatga
       61 gggttacgac tcggcgcgtg cgctatggaa cgcgatggtg gatcgccgcc cggccgtggt
      121 ggtgcggtgc gtgagcagcg cggatgtggt tgctgctatt gattttgcgc ggcgaaatga
      181 cctggagctc ggcatacgtt gcggcggcca cagcgtgttg ggcatctcgg tgccggacgg
      241 cgggttgttg atcgacttgt cggggttgcg cgccgtccgc gttgatgcgg atgcacgccg
      301 ggcttgggtg caaggcggag cgctattagg cgatctggac aaggcgaccc aggcgttcgg
      361 actcgcaacc acggcgggca acgtgtcgca caccggagtg ggcgggttga cgctcggtgg
      421 tggcatgggc tggctcgccc gtcgtttcgg cctggcgtgc gacaacgtcg cctcgtacac
      481 gctcgtcacc gcggacggag aaaccctccg ggttagcgac tctcagcaca aggacctctt
      541 ctgggggctg cgcgggggcg gcggcaattt cggagtagtc accgagttcg agttccggct
      601 acatgaagtg gggacatccg ccgtgctcgc taatcggacc ttcgacctcg cagaggcgcc
      661 gtccgcaatg cggcactggc gtgatctgct cgacgacgac gttccgccag aagcgactcc
      721 actggcttgg gtcggcagga tgccggactc gcccgaggtg cccggggagc tgtgcaaccg
      781 cacagtggcg atcctcggct tcgtatgggt cggtgacccg gagaacggcc gacgctggct
      841 gatgcctcgc ctaactagcc tgggcgtgcc gctggccgag caggtcgagg aagtgaccta
      901 cctcgggttg cagagtacgg acgatgagtg gcaccgcccg ggctcgcgcc ggcgctatta
      961 caagggtcac tatttgacgg agttgtccga tgttgcgatt gatgcgttct tgtcccaggg
     1021 cgaaccggac cctgaagctg accggccgtt attcctgaac ggttccttcc agggctacgg
     1081 cggcgcaatc gctgcaacgt cgaacgacga cagcgccttc agtcaccgca acacacttgt
     1141 cgagtttgtc gctgctacgg cctggacaga cccggccgag gacacccagc gcatggaggc
     1201 tgctcgccgg ttcggtgccg ccatggagcc gttcgccagc ggcgtctacg tcaacgttct
     1261 gggtgacgaa ggagagcttg gtctgcgtcg cgcttatccg gccgagaagc tggatcggct
     1321 ggccgcgctg aagcggcgat acgacccgga caacatcttc catctcaacc agaacatccg
     1381 gccaagctga tcaagggctg agtcccagcg ctcgccgagt cccttgtgct gggcgacgtt
     1441 agcgtctcag caacaagcga agcctggagc caagtaagac cgccacaaag caaagcagca
     1501 gtatggcc
//
LOCUS       DATAOZ010000114         7638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9254426, whole genome shotgun sequence.
ACCESSION   DATAOZ010000114 DATAOZ010000000
VERSION     DATAOZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9254426"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..656)
                     /locus_tag="VHR64_03210"
     CDS             complement(177..656)
                     /locus_tag="VHR64_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEX3301863.1"
                     /translation="MGHRYRMVEKLASFGDDFWIQNDQGQRAFKVDGKMMRMRDTLIF
                     RDMNGNEVCKIQQRIARLRDSMEVEAPGGQRIAMVQKAMIAPLRDRFIVKIGDGPDLE
                     VQGNIVAHEYRIGNVATISKKWFRVRDSYGVEIGPGQNDLLILAATVCIDQMSHDVR"
     gene            complement(917..1408)
                     /locus_tag="VHR64_03215"
     CDS             complement(917..1408)
                     /locus_tag="VHR64_03215"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX3301864.1"
                     /translation="MSTLAVITYPQENTAAEAAASLARMQKSYLIELEDVAWVTKKPD
                     GKLKLHQGTSLTGAAAAGGAFWGFLFGLIFLVPLFGMAVGAATGALAGKLSDFGIDDK
                     WIKEVAESIPAGGSALFVMARNANAEKVLPEMAKFGGTVIKTNLSAQQEQRLSEALTQ
                     AAA"
     gene            complement(1812..2561)
                     /locus_tag="VHR64_03220"
     CDS             complement(1812..2561)
                     /locus_tag="VHR64_03220"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX3301865.1"
                     /translation="MNKRVRTSGIYKRSVTAMLVIASMMLGLLAPIGLQSAVAQDSAS
                     IQTRVQIMHASPDLGKVEVAINYDTKLDEFTYGKVSDWIDIQPGSNRVTITEDRAGFN
                     YAVFDAMYPVPAGNDYYLVISDALVLTSVVDRSPVRDGMARVRVVQASVDLPTVNVKA
                     AGQDVTFGTKLEYARPSEYTTVPAGTYDVEVTLADSGTSVLTMPGISLEGNKVYELVI
                     IGQPDQTDHPLEIKKLEDTTTEQGGVATPSS"
     gene            complement(2804..3310)
                     /locus_tag="VHR64_03225"
     CDS             complement(2804..3310)
                     /locus_tag="VHR64_03225"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX3301866.1"
                     /translation="MTDKPVQLLAAVYPDEEHAKTTIDMLHEMHRAANITLVDSAMIT
                     KNPDGKIKVHETKELTTAKGARRGAIITGVIGLIYPPSLIASALVGGAIGAVAGRVRD
                     TGIKNPKLKEVADQLEPGKAAVIALVEESSTLAAQNALVGYEGSLVIQALDEETVKEL
                     YKAAHPAQ"
     gene            complement(3436..3975)
                     /locus_tag="VHR64_03230"
     CDS             complement(3436..3975)
                     /locus_tag="VHR64_03230"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX3301867.1"
                     /translation="MAAGDFLYVVAASYDDVADARADFESLRGLYNQIGASHDFDAAV
                     LSKNEKGKVKIDKTYEAGTRHDALKGMGWGMAAGVAAAIFPAIGIWGALAAGGAGGAA
                     IGGIAGHYQTGIKRGDLKEMGDMLDKGEAALIAVYRANVADQVSKLIKAENRYVSEVV
                     DATADQIADEIRQAEATYM"
     gene            complement(4183..5700)
                     /locus_tag="VHR64_03235"
     CDS             complement(4183..5700)
                     /locus_tag="VHR64_03235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3301868.1"
                     /translation="MTPINRLPDQKGRVSVPETEDSATLQGRGKLAVIVSSFVFPLAL
                     TQFVASYAASNMNVAISDIADDLGTTTRGVQTAITLFTLTMAALMIPGSKLTDIIGRK
                     RCLMLGLVIYGSGALLASFAQGLGLLIFGYALLEGIGSALMIPPVYIILTVAFSDLES
                     RAKAFGIVSASAGLGAAAGPLIGGLITSAISWRASFIAQVLVVIGILFMGRRFKGTAG
                     VKAPFDITGTVLSAIGLVVIIMGILSTGTYGWGRASEDVKVGDTKIYSEGAIAPVWPL
                     LVIGVLFLAWFFYHISSMEHAGKQPLLSTRVFRNKVSDLGLVTQNVQWLTMQGTFFVV
                     SVFLQTVRGYSAIETGLIMSASTVGLLLSSSAAGRMAEKRSQVDLIRGGFVCMILGTV
                     LMLLLSDATSRIVWFLPGLFLIGFGIGVMLTASVNVVQSSFPEKDQGEISGLSRSVSN
                     LGSSLGTALVGSVLVSKTVPGNHVYAVAIIVMIAIGVVGLGAAMLIPRDAVTKAA"
     gene            complement(5863..6021)
                     /locus_tag="VHR64_03240"
     CDS             complement(5863..6021)
                     /locus_tag="VHR64_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301869.1"
                     /translation="MIERIKIVAVALNLDRIVLALFGWHIMRPRVVPVYVQRSARTPY
                     DYLPDWRR"
     gene            complement(6144..>7638)
                     /locus_tag="VHR64_03245"
     CDS             complement(6144..>7638)
                     /locus_tag="VHR64_03245"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3301870.1"
                     /translation="GLGAALFERDHVVTLRAWLEGLPPEILERSPRLTFWLAWSLGRT
                     GRWTEGNRLLRVSGETWTATNDWLGQGLLLLWHACRSLYALENRKAIEYAERALDTLR
                     EDRAAERVIALMTQGIAHLYRGDPIRAERAFAEVRVIVDTRSLPWLQGFEMSYSAAVL
                     VQQGKLRESTVLSRQVIRATGEAPSEIWVQKALLELGLVQFEWDMLDDAHRTLRRADE
                     LAETTRALQWRARIRVGLARVAWARGEFEDALDEIEQAISFANQLGTLQEVRSARAQQ
                     ARFWIASHQVGLARRWSESCELDPYLPPEYERQVEHLTYVRLLILEERWERALEMLCA
                     IQSEVEASQRRGELVELHILKALAYKAAGQIPEALQSLRYALELGEPSGYCRVFVDEC
                     DELAFLLRHATGRGNYREYASRLLAAIEGTATVQQIDQRSTPDALSEREVEVLRLVAA
                     GMANRDIGQRLFISEKTVKTHLSHILGKLEATNRTQAVDQARRLGVI"
BASE COUNT         1467 a         2410 c         2238 g         1523 t
ORIGIN      
        1 cgtacacagc gtcgaccagt cgcacgattc catcatcgcg gaggcggcgc agttgattca
       61 cgacatcaag agggagggca gattgaccga atccgatcac gactaactgc atcgggccgt
      121 acgtcatgtc gtggtccttt ctgaacattg gttaggtcac cgagtcggag cgaaggtcag
      181 cgaacgtcgt gcgacatttg gtcgatgcaa accgtggcgg cgaggatcag gagatcattc
      241 tgtccaggcc cgatctccac tccgtaggag tcccgcacgc ggaaccactt cttcgatatg
      301 gtcgccacgt ttccgatgcg gtattcgtgg gcgacgatat ttccttgcac ctcgagatcc
      361 ggtccatcac cgatcttgac gatgaagcga tctcgcagcg gagcgatcat ggccttctga
      421 accattgcaa ttcgctgacc accgggagct tcgacctcca tggagtcgcg gagccgggca
      481 atgcgctgct ggatcttgca aacctcattg ccgttcatgt cacggaagat gagggtgtcg
      541 cgcatgcgca tcatcttgcc gtccaccttg aatgcgcgct gtccctgatc gttctggatc
      601 cagaaatcgt cgccgaagga ggccagcttc tcgaccatgc gatagcggtg acccattggc
      661 actgcctgca atccaggcac tggcatcggc cccgcaacat cgggtggggc cgagcctgca
      721 tccctcattg atcttcgtcg gaacatgaga ttgctccttt ttgccaagac cgattcgtcg
      781 gtgtgtcggg cttatcgttg aatcggactt cgctccgacg gttcatctgc cccgtcacat
      841 gtcgaagctc gatcgatgcg cataggtcaa gtacatggcc gccggagaca caccgggatg
      901 tgtccccggc tcatcgctat gcggcggcct gggtcagcgc ctccgagagt cgctgctctt
      961 gctgggccga gaggtttgtc ttgatcacgg tgccgccgaa cttcgccatc tccggcaaca
     1021 ccttctcagc attcgcgttg cgggccatga cgaagagcgc cgatcccccg gcggggattg
     1081 actcggctac ctccttgatc catttgtcgt cgatgccgaa gtcgctgagc ttgccggcca
     1141 atgcaccggt cgccgcacca accgccatcc cgaaaagagg gacgaggaaa atcaggccaa
     1201 agaggaagcc ccagaatgcg ccgccggccg cggcggcgcc ggtcaggctg gtcccctggt
     1261 gaagcttgag ctttccgtca ggcttcttcg tcacccatgc cacgtcttcg agctcaatca
     1321 ggtagctctt ttgcatgcgc gcaagggacg cggccgcctc ggcagccgtg ttctcctgtg
     1381 ggtaagtgat taccgcaagc gtgctcatgg ttcgcctcac tccttctcgt cgacgctcat
     1441 gccagtaaca cggcggttgt ctcgtgccgc cgcacgtggc tccttgtgtc gagcatcacg
     1501 catcgcgcgg aagcccgtac cccccggaac ggaccacgtg gaacatcgcc agaatgtacg
     1561 caggcaggtt gtaccgtcat cagtccaagg gcctaatcgt ctctgtgact gcctgattca
     1621 ggctgatccg atgaggtcgt tggatgggca accggggata ttgagcgacc tttgagacgc
     1681 aacatcgcaa tggggcggag tgcgcttgcg agttgcgctc caccccatcg cgacttccag
     1741 ttgagcaagg atgcttgctc agactgtctc acttgatgtc cgcgccaggc gatgacgccg
     1801 gcgcatgatg cctagctgga cggcgtcgca acgcccccct gctcagtcgt ggtgtcttcg
     1861 agcttcttga tctccagcgg atgatccgtc tgatctggct gaccaatgat caccagctcg
     1921 tagaccttat tgccttcgag cgagatgcca ggcatggtca ggaccgaggt gccagagtcc
     1981 gcgagcgtca cctccacgtc gtaggtccca gccggaaccg tcgtgtactc ggatggccgc
     2041 gcgtactcga gcttggtgcc gaaggtgaca tcctgtccgg ccgccttgac attaaccgtc
     2101 ggcagatcga ccgaggcctg aaccacgcgc acccgggcca tgccgtcgcg gaccggactg
     2161 cggtcgacaa cggatgtcag gaccagagcg tcgctgataa ccaggtagta gtcgttgccg
     2221 gccggaaccg ggtacatggc atcgaagacc gcatagttga agccggcccg atcctcggta
     2281 atcgtgactc gattgctgcc tggctggatg tcgatccagt cgcttacctt tccatacgtg
     2341 aactcatcga gcttggtgtc gtagttgata gctacctcga ccttgccgag atccggcgac
     2401 gcgtgcatga tctggacccg cgtctggatg gatgcgctgt cctgagctac cgccgactga
     2461 agtccaatcg gcgccaacag tccgagcatc attgacgcga tcaccagcat cgcggtcacg
     2521 ctgcgcttgt agattccgga agtccggacg cgcttgttca tcgtcactcc tcctctgcgc
     2581 tctcgagatc accaaactga tgaccgctgg ttgggtccgg gtggtcgagt actcgatctg
     2641 ctggcgcgcc ttgcgcgagg cagacacgga acagcgacgc atccacggga cctggcaatg
     2701 acgctaaggg agttctggtc gtaggcgcat atgccgaatg gagtatttcg tgtcgtgttt
     2761 ccgggacatt tgcctgattc cgtcggggct cgacgagcaa gtgtcactgc gcgggatggg
     2821 cggccttgta cagctccttg acagtctctt catcgagcgc ctggatcacc agcgagccct
     2881 cgtagcccac gagcgcgttt tgcgccgcca gcgtcgagct ctcctctacg agcgcaatca
     2941 cagccgcctt gccgggctcg agctggtcgg caacttcctt gagcttcggg ttcttgatcc
     3001 cggtgtcccg cacacggccg gcaacagctc cgatagcgcc gccgaccaat gccgaggcaa
     3061 tcagacttgg tggatagatc aggccgatca ctccggtgat gatcgcgccg cgccgcgctc
     3121 ccttcgccgt cgtcagctcc ttggtctcgt ggaccttgat cttgccatca gggttcttcg
     3181 tgatcattgc cgaatcgacc agggtgatgt tggcggcgcg gtgcatctcg tggagcatgt
     3241 cgatcgtggt ctttgcatgc tcttcatcgg gatagaccgc cgctagtagt tgaacgggtt
     3301 tgtcggtcat atcgagcgtc ctttctccga gcccgcagtc gaatccaggc gacgacgtgc
     3361 tccggcaggc cctgcacgcg cgctacctgg gatatgaggc gccagctcgt ccggtttgcg
     3421 actgaagctg ctctgttaca tgtaagtcgc ctcggcttgc cggatctcat cggcaatctg
     3481 atcggccgtt gcatcgacga cttcgctgac gtagcggttc tccgccttga tcaatttgga
     3541 cacctggtct gcgacattcg cccggtagac agcgatcagg gccgcttctc ccttgtcgag
     3601 catgtcgccc atttctttca ggtcgccgcg cttgatcccg gtctggtaat gcccggcgat
     3661 accgccgatc gccgcgccgc cagcgccgcc ggcggcaagg gcgccccaga tgccgatcgc
     3721 cgggaagatc gctgctgcca cacccgcggc cataccccag cccataccct tcagggcgtc
     3781 gtgccgggtt ccggcctcgt acgtcttgtc gatcttgacc ttgcccttct cattcttgga
     3841 gaggacggcg gcgtcgaagt cgtgcgaagc gccgatctgg ttgtacagcc cgcgcaggct
     3901 ctcgaaatcg gctcgcgcgt ccgcgacgtc gtcgtacgat gcggctacca cgtatagaaa
     3961 atcacctgcg gccatcagag aagtcctttc gctgatcgtg actgcccagt gcccaaatcg
     4021 agccgaagcc tcgtgggcgc ggcgggtcct ctggccagca tattcagcac aggtaggacc
     4081 gtcataggtc caatggcgta ttcaccgggg cagggaatat cgagcgaaac gcggtttcac
     4141 tcccgcggac ggccgtgaaa tcaagcgacc gaattgcgcc gatcaagccg ccttggtgac
     4201 ggcatcgcgc ggtatcagca tcgcggcgcc gagaccaacc acaccaatgg caatcatcac
     4261 gatgatcgca acggcataca catggttgcc cggtaccgtc ttcgagacca gtacagaccc
     4321 gaccaacgcc gtgcccaggg atgaaccgag attcgagaca ctgcgtgaga gtcccgatat
     4381 ctcgccctgg tccttctccg ggaaactgga ttgcacgaca ttgaccgatg ccgtgagcat
     4441 gacaccgatg ccgaagccga tcaggaagag tcccggcagg aaccagacaa tcctcgaagt
     4501 tgcgtcggat agcaacagca tcagaacggt cccgaggatc atgcagacga accctccccg
     4561 gatcagatca acctgggatc gtttctcagc catcctgcct gctgcggacg aagacaagag
     4621 aagccccacg gttgacgccg acatgatcag gccggtctcg atcgcgctgt agccccgaac
     4681 cgtctgcaga aagaccgaga ccacgaagaa ggtgccttgc atggtcagcc actggacgtt
     4741 ctgggtgacc aatcccaaat cggagacctt gtttcggaag acccgggttg agagcaacgg
     4801 ttgcttgccg gcgtgctcca tcgagctgat gtgatagaag aaccaagcca ggaacaacac
     4861 accgatgacg agaagtggcc agaccggtgc gatcgcaccc tcgctgtaaa tcttcgtatc
     4921 gcccaccttg acgtcttcgc tggccctacc ccagccgtat gttccggtcg agagaatccc
     4981 catgatgatc accaccaggc cgatcgcgga aaggacggtc ccggtgatgt cgaacggcgc
     5041 ctttacgccc gccgtgccct tgaatcgccg tcccatgaaa aggatgccga tgacgacaag
     5101 cacctgcgca atgaacgacg cgcgccaact gatcgcgctc gtgatcagac cgccgatcag
     5161 cggtcctgcc gccgcgccca gtccggcgga cgcactcacg atcccgaacg cctttgcccg
     5221 cgactcgagg tcggaaaagg cgacagtcag aatgatgtac acgggcggaa tcatgagtgc
     5281 cgaaccgatg ccctcgagga gcgcgtaccc gaagatcagc aacccgagtc cctgggcgaa
     5341 cgatgcgagc agcgcgccgg atccataaat caccaacccc agcatcaggc acctcttgcg
     5401 gccgatgatg tccgtcagtt tgcttccagg gatcatcagc gccgccatcg tcagcgtgaa
     5461 gagggtgatc gcggtctgca cgccacgggt ggtggtgccc agatcgtcgg cgatatcact
     5521 gatcgcgacg ttcatgttcg acgcggcgta actcgcgaca aattgtgtca gcgccagtgg
     5581 aaagacgaac gacgagacga tgaccgcgag tttgccccgg ccctgcagcg tcgcggaatc
     5641 ttcggtttcg ggcaccgaga cccggccttt ctgatcgggg agccggttga tgggagtcat
     5701 gaatcaacct ttcgactcgc tggatcgtgc ccaggaacga caagggtcag gtggaaccca
     5761 cgccgggtgc cgactgaccc tttggtcctc agcctgagtt gatcctggga gagcgcatgc
     5821 gttgaacggg ccgacgaccc ggtcagtcct gagaggccaa gcctatcggc gccagtcagg
     5881 caaatagtcg tatggcgtgc gcgctgaccg ttggacgtag accggtacga cacggggccg
     5941 catgatgtgc cagccgaaca gcgcgagcac gattcgatca aggttcagcg caacggcgac
     6001 gatcttgatt ctctcaatca cggctgcatt ctccctcgct ccaattgatc cttgtgaagc
     6061 cactgatgtt tcagacggta cgtacgtgcc ggaacagcgt catcagccca atgacgtatt
     6121 tcgcggactc cgttcctggc cgcttaaatc acgcccaggc gacgggcttg atcgaccgcc
     6181 tgggttcggt tggtcgcctc gagtttcccc agaatgtggc ttagatgggt cttcactgtc
     6241 ttttcactaa tgaagagcct ctgcccgatg tcgcgatttg ccattccggc tgcaaccagg
     6301 cgaagcactt cgacttcccg ctcgctgagc gcatccggag tgctgcgctg gtcgatctgc
     6361 tgcaccgtgg ctgtcccctc gatcgccgcc aggagacgag acgcgtactc tcgatagttg
     6421 ccgcggcccg tcgcatgacg caagaggaac gcgagctcat cgcactcgtc cacgaagacc
     6481 cggcaatagc cgctgggctc accgagctcc agcgcatacc gaagggattg aagggcttcg
     6541 ggaatctgcc cggcggcctt gtacgccagt gctttcagga tgtgaagctc cacgagctcg
     6601 ccacgacgct gtgatgcttc cacctcggac tgaatagcgc acagcatctc cagggcgcgc
     6661 tcccagcgct cctccaggat caagagccga acataggtca ggtgttcgac ctgtcgttca
     6721 tattcgggcg ggagatacgg gtcgagctcg caactctccg accagcgacg ggcaagcccg
     6781 acctggtgcg atgcgatcca gaacctggcc tgctgcgccc tcgcactccg gacttcctgg
     6841 agcgtgccca gttgattcgc gaagctgatc gcctgctcga tctcatcgag tgcgtcttca
     6901 aactccccac gtgcccacgc cacccgcgcc agtccgaccc ggatacgggc gcgccattgc
     6961 agcgctcgcg tggtctcggc caattcgtcc gctcgtcgaa gcgttcgatg agcgtcatcg
     7021 agcatgtccc actcgaattg caccaacccg agctcgagca gtgccttctg tacccagatc
     7081 tcggacggtg cttcgccggt tgcacggatc acctggcggg acagcactgt tgactcgcgt
     7141 agcttgccct gctgaaccag caccgccgcg gagtacgaca tctcgaatcc ctggagccac
     7201 gggagcgatc gggtgtcaac gatcacgcgc acttccgcaa atgcacgttc ggcacgaatc
     7261 gggtcgcccc gatacagatg ggcgatgcct tgggtcatca aggcaatgac tcgttccgca
     7321 gcgcgatctt cccgcaaggt atcgagagct cgttccgcgt attcaatcgc tttacggttt
     7381 tcaagggcat agagcgaccg gcacgcgtgc cagaggagca gcagaccttg gccaagccag
     7441 tcgttcgtgg ctgtccatgt ctcacccgaa acacggagca ggcgattgcc ctcggtccac
     7501 cgtcctgttc gtccgagcga ccaggcgagc caaaaggtca atctcgggga gcgctcaaga
     7561 atttccggcg gcaacccttc gagccaggcc ctcaatgtga cgacgtgatc acgttcgaaa
     7621 agggcagctc cgagtcct
//
LOCUS       DATAOZ010000115         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8728843, whole genome shotgun sequence.
ACCESSION   DATAOZ010000115 DATAOZ010000000
VERSION     DATAOZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8728843"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VHR64_03250"
     CDS             <1..249
                     /locus_tag="VHR64_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301871.1"
                     /translation="EIERVEVDDLDDRLATTPGALVIADRTSNAIDQRAAACILYYPD
                     QRNVAIVRSGSAPRTIENPAWEDMLSAIDSAIAGMAGV"
     gene            367..1011
                     /locus_tag="VHR64_03255"
     CDS             367..1011
                     /locus_tag="VHR64_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3301872.1"
                     /translation="MLVDDHASLRRPLAFMIEREPDLTVVAEAGSLAEARAHLQAGIS
                     PDAVILDLNLPDGDGTDLIRDLRRVNPLANTLILSGVVDVRSRARAVAAGAGAVFPKT
                     TEIEELIDAIRRMRQGEVLISPAEALELVRYAEQVGREDRIVAVGVAELTPREREILR
                     ALADGLSDKEIAERLYIGDKTVRNHVTSMLSKLGAESRLQALVIAIRHGVVRVE"
     gene            1183..>2037
                     /locus_tag="VHR64_03260"
     CDS             1183..>2037
                     /locus_tag="VHR64_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301873.1"
                     /translation="MNSLDSSHRSSLAHRVITLATLFALIAMLLSPLATLAQTADGTP
                     VDPPPAEEPAPDTNVPADPTATVEPTTAPVETVAAPEATNTEAPAPTETTAPAPADTV
                     APEATSTTAPEPTATRTFTEPPTIQSDKDDYPPGGRVTLTGANWQPGEVVHIYVNDDH
                     GSSWSRNVDVIADETGHITDAFNLPDWFVAEYSVTATSLLSGRATSYFTDSDIKVVAG
                     GAGVQFTLSYSTVDNTVGTQTQCEAAATPNSQPVDGTGFNITVQNGRWIKLSAPATSA
                     AGGAFVSWT"
BASE COUNT          386 a          665 c          633 g          353 t
ORIGIN      
        1 gagatcgagc gggtcgaggt cgatgatctc gacgatcgcc ttgccacgac gcccggggca
       61 cttgtgatcg ccgatcgcac cagcaacgcg atcgaccagc gggccgccgc ttgcatcctc
      121 tattacccgg atcagcgcaa cgttgcgatc gtgcgatcgg gatctgctcc gcgcacgatc
      181 gagaaccccg cctgggagga catgctctcg gcgatcgact ccgcgatcgc tggtatggcc
      241 ggcgtctagg accaatatct caagtcgcta tatccaaatg ggccatgccg gtcgtatgct
      301 ctgaaggatc gattcgtcga gcaatcggag cgttgcccag tgtccgcctc ctcacccacc
      361 cgcgtgatgt tggtagacga tcatgcgtca ttgcgccgcc cgctcgcctt catgatcgag
      421 cgagagcctg atctgacggt cgtcgcggaa gccggctcac tggccgaggc gcgggcgcac
      481 cttcaggccg gcatatcgcc cgatgccgtg atcctcgatc tcaatctccc ggatggagac
      541 ggtaccgact tgatccgcga cctgcgcagg gtcaatccgc tcgcgaacac gctcatcctc
      601 tcaggcgtgg ttgacgtgcg atcccgcgct cgggctgtgg cggccggagc gggagcggtc
      661 tttccgaaga cgaccgagat cgaggagctg atcgacgcga tccggcggat gcgccagggc
      721 gaggtgctga tctcgcccgc cgaggcgctg gagctcgtgc gatacgcgga gcaggtcggc
      781 cgcgaggacc ggatcgtcgc ggtcggcgtg gccgagctga cgccgaggga gcgggagatt
      841 ctgcgtgcgc tggccgatgg attgagcgac aaggagatcg ccgaacgcct ctacatcggc
      901 gacaagacgg tccgcaacca cgtgaccagc atgctctcga agctgggcgc ggagtcccgc
      961 ctgcaggcgc tggtgatcgc cattcggcac ggggttgtcc gcgtcgagta gcccaaaggt
     1021 cctgcgtgaa atcccccaac gagaatcggg acatttgacc cggcccgaac ggctgacccg
     1081 ttattactat tcctgttaat ccgtattgcg tgtcccaacg gatcgtgccg agggtgttcc
     1141 cgcggctata ggaacctgtg cgccttcgta gggagaaccc gtgtgaacag tcttgactcc
     1201 tcgcatcgca gcagcctggc tcaccgagtc atcacgctgg ccacgctctt cgcgctgatc
     1261 gcgatgctcc tcagtccgct tgccacgttg gcgcagacgg ccgacggcac gccagtggat
     1321 ccgcctcctg cggaagagcc tgctccagat acgaatgtgc cggccgaccc gacggcgaca
     1381 gtcgagccga ccacggcgcc ggtcgagacg gtagcggcgc cagaggcgac gaatacggaa
     1441 gcgcccgcgc cgaccgagac cactgccccg gctccggccg atacggtggc tccggaagcg
     1501 accagcacca cggcgcctga gccgaccgcg acccgcacct tcaccgaacc gccgacaatc
     1561 cagagcgaca aggacgacta cccgccgggt ggtcgcgtca cgttaaccgg cgccaactgg
     1621 cagccgggcg aagtcgttca tatctatgtc aacgacgacc acggcagctc ctggtcccgc
     1681 aacgtcgacg tcatcgcgga cgagaccggc cacatcaccg acgccttcaa cctccccgat
     1741 tggttcgtgg ccgagtacag cgtcactgca acgagtcttc tttcgggaag agcaacatct
     1801 tactttacgg atagcgacat caaggtagtt gctgggggcg caggagtcca attcacactt
     1861 tcatacagca ctgttgacaa cactgtcggg actcagaccc agtgtgaagc tgctgctact
     1921 ccgaacagcc agccggtgga cggtaccggc ttcaacatta ctgtccagaa cgggagatgg
     1981 ataaagctct ccgcaccggc aacctcggca gccggcgggg catttgttag ttggact
//
LOCUS       DATAOZ010000116         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4256029, whole genome shotgun sequence.
ACCESSION   DATAOZ010000116 DATAOZ010000000
VERSION     DATAOZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4256029"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VHR64_03265"
     CDS             complement(<1..948)
                     /locus_tag="VHR64_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3301874.1"
                     /translation="MRLIRNFSIIAHVDHGKSTLADRFIQTCGGLQAREMQDQVLDSM
                     ELERERGITIKAQSVTLYYQAADGQRYQLNMIDTPGHVDFSYEVSRSLAACDGALLVV
                     DASQGVEAQSVANCYTAIEQGLEVVPVINKIDLPSADPERVMQEIEDIIGIEATDALK
                     VSAKTGEGVPELLEALIRRIPAPKGDPDGPLQALIIDSWFDNYVGVVSLVRVVNGSLK
                     TGDKIRVMSTGRSHQVDKVGRFTPKSVAEKELVTGDVGFVVAGIKEIDGAPVGDTLTL
                     EHRPSATPLPGVKQIKPRVFAGLFPVNSEDYESFRDALAK"
     gene            complement(1075..1344)
                     /locus_tag="VHR64_03270"
     CDS             complement(1075..1344)
                     /locus_tag="VHR64_03270"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX3301875.1"
                     /translation="MGADLSSAVSRLTVVHRQGCDLCDEMVAELQALGRNLKLPPIEL
                     VDVDADAQLLRRHGMEVPVLLLDGTVVCRHRLDSEELQRLLRPSP"
     gene            complement(1351..>2120)
                     /locus_tag="VHR64_03275"
     CDS             complement(1351..>2120)
                     /locus_tag="VHR64_03275"
                     /inference="COORDINATES: protein motif:HMM:NF028497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HEX3301876.1"
                     /translation="FPTLNDQTASVYEIKEVERMRLNRRDTHVIVVSPKDEFRYGYRL
                     WIDERTALPQKTQLCDARGHVIEQIVFSELAQTSHIPDIAFKPDVSTDGFQWLRNETP
                     VGKEPAASASIVWNALKLPPGFKMRAHSAQVLPGSTDPVDHVVFTDGMASVSVFVETR
                     PKPAAGQPQTVEEETATVGSSSAYSTVSNGRKVTAVGEVPPATVRFIANSVRAQGGQS
                     TGGAAPSLGNTPAPSMPSSIAGGGGAPQPVMGGGGRR"
BASE COUNT          368 a          691 c          679 g          382 t
ORIGIN      
        1 cttcgccagc gcgtcgcgga aactctcgta atcttcggag ttgaccggaa agaggcccgc
       61 gaagactcgc ggcttgatct gtttgactcc cgggaggggg gttgcgctcg ggcggtgctc
      121 cagggtgagc gtgtcgccca ccggagcgcc atcgatctcc ttgatgccgg ccaccacgaa
      181 accgacatcg ccggtcacca actccttttc cgcaacggat ttcggagtga agcgcccgac
      241 cttgtcgacc tgatggctgc ggccggtgga catcacacgg atcttgtccc cggtcttgag
      301 gctcccgttg acgacgcgca ccagcgacac gacgcccacg tagttgtcga accaggaatc
      361 gatgatcagg gcctgcagcg gcccgtcggg atcgcccttg ggcgccggga tgcggcggat
      421 gagcgcctcc agcagctccg gcacgccctc gccggtcttc gcgctgacct tcagggcatc
      481 ggtggcttcg atgccgatga tgtcctcgat ctcctgcatc acccgctcgg gatcggccga
      541 cggcaggtcg atcttgttga tgaccggaac gacctcgagc ccctgctcga tcgcggtgta
      601 gcagttggcg acgctttgcg cttccacacc ctgcgaggca tcgaccacca gcagcgcgcc
      661 atcgcaggca gcgagcgacc gcgagacctc ataggagaag tccacgtggc ccggcgtgtc
      721 gatcatgttc agttgatagc gctgaccgtc cgccgcctgg taatacagcg tgacgctctg
      781 cgccttgatt gtgatacccc gttcgcgctc gagctccatc gagtcgagca cctgatcctg
      841 catctcgcgg gcctgaaggc cgccgcaggt ctggatgaac cggtccgcga gagtcgactt
      901 gccgtggtcg acgtgagcga tgatcgaaaa gttacgaatg agccgcatga atcaaggagc
      961 ctgtctgacc tggtctgccg cgttcgccgc gccagtaccg tagcggcgcg cggattatag
     1021 cggcgttcgg ttatgcgtgt gagccgctta ggcgagtcgc cgaaggctgg ttcttcaagg
     1081 acttgggcgt aatagccgct gtaattcctc gctatcgagc cggtgcctgc agaccactgt
     1141 gccatcgagc agcaggaccg gaacctccat tccatgacgg cgcagcagct gggcatccgc
     1201 atcgacgtcg acgagctcga tcggagggag cttcaggttg cgccccagcg cctggagctc
     1261 ggcgaccatt tcgtcgcaca gatcgcagcc ctggcgatgc acgacggtca gacgcgagac
     1321 ggcggaggac aggtcggctc ccaaggacgc ttaacgccgg ccgccgccgc ccatcacagg
     1381 ctgcggggct cctccacccc ctgcgatcga ggacggcatc gaaggcgcgg gcgtgttgcc
     1441 caacgagggg gccgcgccgc cagtgctctg gccaccctgg gccctcacgg aattcgcgat
     1501 gaaccgcacc gtcgccgggg gcacttcgcc caccgcagtc actttgcgcc cgttgctcac
     1561 agtggaataa gccgacgacg aaccgaccgt cgcggtctct tcctccactg tctggggttg
     1621 tccggcagcc ggcttgggtc gcgtctcgac aaagaccgac acggaggcca tgccatcggt
     1681 gaatacaacg tgatccaccg gatcggtcga gcccggcaac acctgcgccg agtgcgccct
     1741 catcttgaac ccgggtggca gcttcaacgc attccagacg atggaggcgg aggcggcagg
     1801 ctccttgccg accggagtct cattccgcaa ccactgaaag ccatcggtcg agacgtcggg
     1861 cttgaacgcg atatcgggaa tgtgcgaggt ctgggcgagt tccgagaaca cgatctgctc
     1921 gatcacgtgc ccgcgggcgt cgcagagctg agtcttctgc gggagcgccg tccgctcatc
     1981 gatccacagg cggtagccgt agcggaactc gtccttcggc gaaacgacga tgacgtgcgt
     2041 gtcgcgccgg ttcaggcgca tccgctcgac ctccttgatc tcgtagacgg aggcggtctg
     2101 atcgttcagg gtcgggaagt
//
LOCUS       DATAOZ010000117        11119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7941086, whole genome shotgun sequence.
ACCESSION   DATAOZ010000117 DATAOZ010000000
VERSION     DATAOZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7941086"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..1605)
                     /locus_tag="VHR64_03280"
     CDS             complement(310..1605)
                     /locus_tag="VHR64_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX3301877.1"
                     /translation="MQPESNNERAWNIALAISIALFIGLSSFAAGIIAERNYFSNREN
                     GNNLGRAEQVHDLIEGEYFAAPTDPTARAEFDTKLEDAAITGMMGVLDAHSQFLPPAD
                     TASVNQQLSGTYQGIGVWSDIVDNKLVVIPMPGSPAEEAGIKAEDVILAVDGTPVATD
                     GVDTAVESIKGPAGTTVTLSIERANQNPFDVSVTRREIPNYSVFYRVIPGTNIAHIQV
                     SIFGSATIDELDAVLDRLDQEGVTGIVLDLRNNGGGLVSAAQEMIGRFVPESSGPALI
                     EDDSAAPGDETKIPILSDGRTPVTLPMVVLVNGGTASASEIVAGALQDYHRATIVGEQ
                     SFGKGSVQRVHDLSDGSSVRITFAQWLTPTGRLIEGSGITPDVAVTAPTTPDSPDTQL
                     DRAVEVVSGAPGTPVAASPQAATPVATPATPSTVATPAI"
     gene            1716..2486
                     /locus_tag="VHR64_03285"
     CDS             1716..2486
                     /locus_tag="VHR64_03285"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX3301878.1"
                     /translation="MFLAIDVGNTHTVVGLLTPNGHWCARWRITTVRNPLGADWAPAF
                     ASLAGRDNIDLHAIKAVCICSVVPTATTALTEFAREWLKVEPMIVRSTQNLNIRLGMD
                     NPHEVGADRIANAVAAWDLCRSACVVVDLGTATKVEAISDEGVFLGGSIAAGIGASME
                     ALTSRAARLFNIDLTLPESAIGRNTVEALQAGIVLGHRHLVGGLVTDIRGIIGVDAVV
                     LLTGGYAAREDSPFRFLWQHEPDLTLNGIRLIHELNSV"
     gene            2741..4804
                     /gene="fusA"
                     /locus_tag="VHR64_03290"
     CDS             2741..4804
                     /gene="fusA"
                     /locus_tag="VHR64_03290"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX3301879.1"
                     /translation="MKQYSPEHIRNVAVVGHGSAGKTTLVEAMLFTAHATTRMGKPED
                     GNTVSDYDQDEIKRRMSITAAVAPLEWRDTKINLIDTPGYSDFVGEVKAAIRVAESAL
                     IVADAASAVEVGTENGWRFACDAGVARMVFVNRMSRENADFDRTVDGLRAAFGKSLVP
                     VQFPIGKEKDFRGLVDLLHEEAHLYGDSQSGESQVVPIPDEVMDDCRKYRQMLVETVA
                     EQDEELMMKYLDDQDLSIEEMLPVLRRAVKAGDLAPIFCGSSTQNRGIDLLLDQIVEM
                     APDASPWVEGEEHAAENGNALQSLIFKTIADPHVGQVSYFRVFRGAFNANSHVFNSVQ
                     EQGERIGQIFYVRGKEHISTDTVGTGDIGAVSKLAHSHTNDTLVADGLTMTLEPVQFP
                     LPSYAAAVHPRSKSDLDKLGQALNRMVEEDPTLQLSRDPITGESILSGLGESHVQIAL
                     DRMSRRFGVNVDAGLPRVAYRETVSAKTNSEYKHKKQTGGAGQYGHVYLEIEPLPDAN
                     FEFSERVVGGSVPRNFFPAVEKGVREALEAGPVAGYPVVNVKVTLYDGSYHDVDSNEM
                     AFKIASKEAFKKGVLQAKPTLLEPMMTLKVTVPDQYTGDVMSDLNGKRAQVSGMNPDG
                     DGKTTVEAVVPAAEVQRYATDLRSITQGRGSFTTEFSHYQPVPPMIAEQIKEAANREH
                     EGAAA"
     gene            4896..5303
                     /locus_tag="VHR64_03295"
     CDS             4896..5303
                     /locus_tag="VHR64_03295"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX3301880.1"
                     /translation="MEPRSIKITFDGGALGNPGKGYGSYLIEGLTSDPAVARLDFSPN
                     GELVTNNQAEYRTLINAMRSLVDSDVVDARALDLEICGDSKLVIEQLAGRWKVKHPDI
                     RPLHSEASNLMRQFHRVTLNWHRRDVSVDKLGH"
     gene            5306..5773
                     /locus_tag="VHR64_03300"
     CDS             5306..5773
                     /locus_tag="VHR64_03300"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3301881.1"
                     /translation="MGTRPASLQTGQAIRAAVVDNGGKRLALGRAAAGRRQMLVFERG
                     HWCATCRRHLGLMTEHVAEFTARNIDIVAITHEPESDLNGRVYPFPIIPDPSLELAAE
                     FDVVGVDEFGKRTIRPATILVDDQGMIRFSYVGDDSRDRPTIPALLLALDTLI"
     gene            complement(5744..6049)
                     /locus_tag="VHR64_03305"
     CDS             complement(5744..6049)
                     /locus_tag="VHR64_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301882.1"
                     /translation="MSNDRRANPPEERDLLDEAMELPLVGAGVTRLQRVLGPSCLASL
                     VVLLLLTVLFRRWVHLPPVGMLILLIVVWMMILAVMVRFRNPNPDGEDSDQGIQRKQ"
     gene            6098..6171
                     /locus_tag="VHR64_03310"
     tRNA            6098..6171
                     /locus_tag="VHR64_03310"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6132..6134,aa:Val,seq:gac)
     gene            complement(6182..6955)
                     /locus_tag="VHR64_03315"
     CDS             complement(6182..6955)
                     /locus_tag="VHR64_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX3301883.1"
                     /translation="MNPSELRRALADGEPVFGTMIFEFNTPGVGLIAAEAGASFVLFD
                     MEHTGWSIESIRAQMAWSRSAPITRLVRVPATQYDYISRALDVGAEGVMVPMVESAEQ
                     AKSIAAASLYPPDGRRGAAVGIAHDGYAPGPVAEKMTRANAERIVLAQIETAAGVRNA
                     AEIAQIPGIDVLWVGQFDLTVSLGIPGQFDHPHFVEAVSTVAKAAKAAGKACGAMALS
                     VDDATVWAKRGYDMLAYSGDLWIYQQALTEAMNRLRKAL"
     gene            complement(6952..7977)
                     /locus_tag="VHR64_03320"
     CDS             complement(6952..7977)
                     /locus_tag="VHR64_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3301884.1"
                     /translation="MGKFVVGVGSDFYDAEGVPKLPALRSAFLDLPDDVEIRTVQCNG
                     GTYQCDAIRDIDALLMSGGSCDASSLLDNDRLAVVCRWGVGYDTVNLAECAEAGVVVV
                     NAPEGVRRSMAQTAMAFVLCLAHRIFDQDRAMRSGNLWSDKHQFIGTGLVGKTLGVIG
                     LGNIGREILRVASVLDCEVIGYDPYADQSAGPVTRVELDELMARSDFIIIQCALTPET
                     RGMINRDRIAMMKPTAYLVNTARGPIVDEPALIEALQHRKIAGAALDVFAREPLENDS
                     PLLKMDNVILTPHSAGWTHDFARRTAESISTSIRSIMNGEMPANAVNKPELEAAGVSP
                     RYLRNRP"
     gene            8173..9297
                     /locus_tag="VHR64_03325"
     CDS             8173..9297
                     /locus_tag="VHR64_03325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX3301885.1"
                     /translation="MHGQSPDPATLDQISSKRYIVIDDQTGEIYAQKDADTRGGIASL
                     TKVFTAIVALEHASLDTQITANESDEFDSTSTRMTGFAPGTTYTVRDLLYGMILESGN
                     DAAHALARGVGAQPGDSGEDSVNRFVGWMNDKVTALGLTDTHFVNPHGLSDPDHYSTP
                     RDIATFMMYAVQNADFMAIITARNYTTTTGATLTSVNRGPEFIPDYIGGKTGYDDATG
                     YCLVEIGQRDDAQLVSVTIDGVAPEVWYEDHAVLQEYGFAARSERIAAGQPLGDVLAY
                     VGQPGSEQAQVDEPVEQQTPPASDIFAGVETGPRPVLVTGTPVPFAAVNDGGDSPYHN
                     WLIGLVIAAIVTGSLWMHAGMKLRFVARRPMPEPGVEIDT"
     gene            9590..>11119
                     /gene="thrS"
                     /locus_tag="VHR64_03330"
     CDS             9590..>11119
                     /gene="thrS"
                     /locus_tag="VHR64_03330"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX3301886.1"
                     /translation="MAEVKEPGSDVVVEIENEQDLALATMRHSAAHLMAEAVRELIPE
                     VKFAIGPAIENGFYYDMDLPRPLSPDDLQAIENLMARHREADEPFVRTEVSKAQALET
                     FKNNPYKVEILKELPDDSTITTYQQGTFLDLCKGPHVESTGKIGAFKLLSVAGAYWRG
                     DEKRPMLQRIYGTAWPTQEELDDYLARIEEAQRRDHRRLGRDLDLFSVNDEIGPGLIL
                     WHPRGAMIRYQVEQFEQKEQLERGYQIVYTPHIASEKIYQTSGHLETYRENMYSPMSI
                     EEVDYYLKPMNCPGHIKIYQSHVHSYRDLPIRYAELGTVYRYERSGTLHGMLRVRGFT
                     QDDAHIFCTEEQMIDEIIGVIDLATYMARVFGYDFKAYLATRPDKWVGDEQTWAHATD
                     ALKLALEQKGVDYKMDEGGGAFYGPKIDIKWVDALGREWTGPTVQLDFNLPQRFDINY
                     IGEDGEKHRAVMIHRTLLGSMERFVGGLVEHYAGAFPVWLAPTQIALIPITDDQVEYC
                     QN"
BASE COUNT         2145 a         3534 c         3431 g         2009 t
ORIGIN      
        1 tgatgatacc gatcgtcgtg gctaccgaga gcaccgcaca ggcgccgaga aggatcccga
       61 ttacgcgttc ggatccgcgc cggctacccg atccctggag cgcctttgcg atctccagcg
      121 agcgggactc tgtggggaca accgcctcta gcgccatgtc ccggctcccg tgatgaggca
      181 gtggattgtg cggggaatga acgtcacgtc caggtacggg aaatcgtgtc gtgaagtcgt
      241 catgacctca ctctaggggc gtcctgttaa cggctcgtta tcgcccggta aacgattctg
      301 aacagattgt taaatcgccg gtgtcgcgac cgttgaggga gtggccggcg tggccaccgg
      361 agtcgccgct tgaggcgacg cggcgaccgg agttcctggc gcacccgaca caacttcgac
      421 ggcgcgatca agttgcgtat caggcgaatc gggtgtcgtc ggcgcagtta ctgcgacgtc
      481 cggcgtaatt ccggaaccct cgatcaaccg gccggttggc gtcagccact gcgcaaaggt
      541 aattcgcaca ctcgacccat cggacaggtc gtgcacgcgc tggaccgacc ccttgccgaa
      601 gctctgctcg ccgacgatcg ttgcccggtg gtaatcctgc agcgcgccgg ccacgatctc
      661 ggaggcgctg gcggttccgc cgttgacgag gacaaccatc ggcagcgtta ccggagttcg
      721 gccgtcgctg aggatgggga tcttcgtctc gtcgcccggc gccgccgagt catcctcgat
      781 cagggccgga cccgagctct cgggaacgaa ccgcccgatc atctcctggg ccgcgctgac
      841 cagtccaccg ccgttgttgc ggaggtcgag cacgatgccc gtcacgccct cctggtcaag
      901 gcgatcgagc acggcgtcca gctcatcgat cgtcgctgag ccgaagatgc tgacctggat
      961 gtgggcgatg ttggtgccgg ggatcacgcg gtagaagacg gagtagttcg ggatctcgcg
     1021 ccttgtcact gagacatcga acggattctg gttcgctcgc tcaatgctca atgtgacggt
     1081 cgttccggct gggcccttga tcgattcgac ggccgtatcg accccgtcgg tcgcaaccgg
     1141 cgttccatcg accgcgagga ttacgtcctc ggccttaatg cctgcctcct ccgccggcga
     1201 gcccggcatc gggatgacga ccagcttgtt gtcgacgatg tcgctccaga ccccaatgcc
     1261 ctggtaggtg ccgctcagtt gttgattgac gctggccgtg tccgccggcg gcaggaactg
     1321 ggaatgtgcg tcgaggaccc ccatcatgcc ggtaatcgcg gcgtcttcca gcttggtgtc
     1381 gaattcggcg cgagcggtcg ggtcggtcgg agcggcaaaa tattcgccct caatcaagtc
     1441 gtgaacttgc tctgctcggc cgagattgtt cccgttttcg cgattgctga agtagttgcg
     1501 ctcggcaatg atcccggccg cgaaactgct caatccgatg aaaagggcga tgctgatcgc
     1561 aagcgcaatg ttccaggcgc gttcgttgtt cgattccggt tgcattggat ccccaggcag
     1621 tccgcaaaat tgcctgccaa gtctagccgt tcccgacccg ggaatatgtc gacatatact
     1681 gcggatatcg acgatttcac tcggatgagg ggtccatgtt tctcgcgatc gatgtcggca
     1741 atacgcatac ggtcgtcggc ctgctgacac ccaacggaca ctggtgcgcg cgctggcgga
     1801 tcacgactgt ccgcaatccg ctcggcgccg attgggctcc ggcctttgca tccctggccg
     1861 gtcgcgacaa tatcgacctc cacgccatca aggcggtctg catctgctcg gtggttccga
     1921 ctgcgaccac ggcgttgacc gagtttgcgc gcgaatggct caaggtggag ccgatgatcg
     1981 tgcggtcgac ccagaatctg aatatcagac tcggcatgga caacccgcac gaagtcggcg
     2041 ccgaccgcat cgcgaatgcc gtcgccgctt gggacctctg ccgatccgcg tgcgtcgttg
     2101 tcgatctcgg tacggccacc aaggtcgaag cgattagcga cgaaggtgtc ttcctgggcg
     2161 gttcgatcgc ggccggaatt ggcgcgtcga tggaggcgct cacatcccgc gcggctcggc
     2221 tcttcaacat cgatctcacc ttgccggagt ccgcgattgg ccggaacacc gtcgaggcgc
     2281 tccaggcggg catcgtgctg ggccaccgtc acctggttgg gggcctggtc acggacattc
     2341 gcgggatcat tggggtcgat gcagtcgtgc tcctgaccgg tggttatgcg gcgcgggagg
     2401 actccccatt ccgcttcctc tggcagcacg aacctgacct gacgctgaac ggaattcgac
     2461 tgatccacga gctgaattcc gtataggacc gattcggctt gacatcggcc ccgggtgttc
     2521 aataattcga caaaccttgc cggacgcggc gtgatccaaa acgtgaacta cgcgcgaggc
     2581 gggatgtgat ggaaatcctg tacgcgggcg tgcgacggta gccatccacc tggaacccgg
     2641 gccgacgatt gatccgtctt cgccgtcagg gatgaacgag cgccagggcg gacattctcg
     2701 gcgttcgacg cgctggagcg tcgagacgag gagtcttgcc ttgaaacagt attcccctga
     2761 acacatccgc aatgtggctg tggtcggtca cggcagtgcc ggaaagacga cccttgtcga
     2821 ggcaatgctc ttcaccgcgc acgcaaccac tcgcatgggt aagccggaag atggaaacac
     2881 cgtcagtgac tatgaccagg atgagatcaa gcgccgaatg tcgatcacgg cggcggtcgc
     2941 gccgctcgaa tggcgtgata cgaagatcaa cctgattgat acgcccgggt attcggattt
     3001 tgtcggcgaa gtcaaggcag cgattcgtgt ggccgagtcc gcgctgattg tcgcggatgc
     3061 cgcatctgcc gttgaagtcg gcaccgagaa tggctggcgg ttcgcgtgcg atgccggcgt
     3121 cgcccggatg gtcttcgtga atcggatgag tcgcgagaac gccgacttcg accgcacggt
     3181 cgatggcctc cgtgccgcct tcggcaaaag cctcgtgccg gtccagttcc cgatcggcaa
     3241 ggagaaggac ttccgcggcc tcgtcgacct ccttcacgag gaagcgcacc tctacggtga
     3301 cagccagtcg ggcgagagcc aggtcgtgcc aataccagac gaggtcatgg acgactgccg
     3361 gaagtatcga cagatgctgg tcgagaccgt cgccgagcag gacgaagagt tgatgatgaa
     3421 gtacctggat gaccaggatc tctccatcga ggaaatgctc ccggtcctgc gcagagctgt
     3481 gaaggccggc gatctggcgc ccatcttctg cggctcatcg acgcaaaacc gcggcatcga
     3541 cctgctgctc gatcagattg ttgagatggc tcccgatgca tccccttggg tggagggcga
     3601 ggagcacgcg gccgaaaacg gcaatgcgct acagtccctg atcttcaaga cgattgccga
     3661 ccctcatgtt ggccaggtct cgtatttccg cgtcttccgc ggcgcgttca acgccaactc
     3721 gcacgtcttc aactcggtcc aggagcaggg cgagcggatt ggccagattt tctatgtgcg
     3781 cggcaaggag cacatctcga ccgacacggt gggtaccggc gatatcggcg ctgtcagcaa
     3841 gctcgcgcat tcgcacacga acgacacgct cgttgctgat ggcctgacaa tgacgctgga
     3901 acccgtgcag ttccccttgc cgtcgtacgc cgcggccgtc catccgcggt cgaagtcaga
     3961 cctggacaaa ctcggccagg cgctgaaccg gatggtcgaa gaggacccaa cccttcagct
     4021 cagccgcgat ccgatcaccg gcgagtcgat cctctccggc ctcggtgaat cgcacgtgca
     4081 gattgcgctc gatcgcatga gtcgccggtt cggcgtcaat gtcgatgccg ggctgccgcg
     4141 ggtcgcctat cgcgagaccg tctcagccaa gacgaactcc gagtacaagc acaagaagca
     4201 gacaggtggc gccggacagt acggtcatgt ctatctggag atcgagccgc tgcccgatgc
     4261 caacttcgag ttcagcgagc gggtcgtcgg cgggtccgtg ccgaggaact tcttcccggc
     4321 cgtcgagaag ggagtacgcg aagcgctcga ggcagggccg gttgcaggct atccggtcgt
     4381 gaacgtcaag gtgacgctct acgacggctc gtaccacgat gtcgactcga acgagatggc
     4441 cttcaagatc gcctcgaagg aagccttcaa gaagggcgtc ctgcaggcca agccgacact
     4501 gctcgagccg atgatgaccc tcaaggtcac cgtcccggat cagtacaccg gagacgtcat
     4561 gagcgacctc aacggaaagc gcgcgcaggt gagcggcatg aaccccgacg gtgacggcaa
     4621 gaccaccgtc gaggcagtcg tgccggcggc cgaggtccag cgatacgcca ccgatctccg
     4681 ttcgatcacg cagggacgcg gctcgttcac caccgagttc tcgcactacc agccggtccc
     4741 gccaatgatc gcggagcaga tcaaggaggc ggccaaccgc gagcacgaag gcgccgcggc
     4801 ataggtccgg aacctgcgaa aatagcgtga ccggtcgacg ccgaccggtc actttttgtg
     4861 ccggctacgt gtcatgctat ggagcgattt cgtccttgga accacgatca atcaaaatca
     4921 cgttcgacgg cggcgcgctc ggaaacccgg gcaagggtta tggcagctac ctgatcgaag
     4981 gactcacatc cgatccggcc gtcgcgcggc tcgacttcag tcccaatggc gagctggtca
     5041 cgaacaacca ggcggaatat cggacactga tcaatgcgat gcggagcctg gtcgattccg
     5101 atgtggtcga tgcccgggcg ctcgacctcg agatctgcgg tgacagcaaa ctggtgatcg
     5161 agcagcttgc gggtcggtgg aaagtcaagc accccgatat ccggccgctg cactccgagg
     5221 caagcaacct catgcgccag ttccaccgag taacgctcaa ttggcatcga cgcgacgtct
     5281 ccgtcgacaa gctgggccac tagtcatggg cacgcgaccc gcatctctgc aaaccggcca
     5341 ggcaattcga gccgcggttg ttgacaatgg cggcaagagg ctggcgctgg gtcgggctgc
     5401 ggctggacgc aggcaaatgc ttgtcttcga gcgtggacac tggtgcgcaa cctgccgccg
     5461 acacctcgga ttgatgaccg agcacgttgc cgagttcact gctcgcaaca tcgatatcgt
     5521 cgcaattacg catgagccgg agagcgacct gaatggccgg gtctacccgt tcccgatcat
     5581 ccccgacccg agcctggaac tcgccgccga atttgatgta gtcggcgtcg acgagttcgg
     5641 caagcggacg atcaggccgg cgacaatcct cgtcgacgat cagggaatga tccggttcag
     5701 ctacgtcgga gacgattcgc gcgatcgccc gacaatcccg gccctactgc ttgcgctgga
     5761 taccctgatc tgagtcttcg ccatcgggat tcggattccg gaagcgcacc atcacggcca
     5821 ggatcatcat ccagaccacg atgagcagga tcaacatgcc gaccggcggg agatgcaccc
     5881 agcgccggaa caacaccgtc aacaacagaa gcacgaccag gctggccagg cagcttggac
     5941 cgagcacgcg ctggaggcga gtcacgccgg ccccaaccag cggcagctcc atggcctcgt
     6001 cgagaaggtc gcgctcttcc gggggatttg ctcgtcgatc gttgctcaat tgacaccttc
     6061 aggcgcggct ccgtatactc gcgcctgcac ccattcgggg cgattagctc agttggttag
     6121 agcgctacgt tgacatcgta gaggtcagtg gttcgagtcc actatcgccc accggcaccc
     6181 ctcacaacgc cttcctcagt ctattcatcg cttcggtcag cgcctgctgg tagatccaga
     6241 ggtcgccgga ataggcaagc atgtcgtagc cgcgtttggc ccagaccgtc gcgtcgtcga
     6301 ccgagagtgc catcgctccg cacgctttgc cagcggcctt cgccgccttg gcaacggtcg
     6361 agacggcctc gacgaagtgc gggtgatcga actgacccgg gataccgagc gacacggtca
     6421 aatcgaattg gccgacccaa agtacgtcga tccccgggat ctgggcaatc tcagcggcat
     6481 tccgtacgcc ggcagcggtc tcaatctggg cgaggacgat gcgctcggca ttggcccggg
     6541 tcatcttctc cgcgacggga cccggcgcgt aaccatcgtg cgcaatcccg actgccgcac
     6601 cacgccggcc gtcgggcgga taaagagacg cggcggcgat cgacttcgcc tgctcggccg
     6661 actccaccat cggcaccatc acgccctccg caccgacgtc gagtgcgcgg ctgatgtagt
     6721 catactgcgt ggccggcacg cgaaccagcc gggtgatcgg cgcggatcgg gaccatgcca
     6781 tttgcgcgcg aatcgattcg atgctccaac cggtgtgctc catgtcgaag agcacgaacg
     6841 atgctcccgc ttcggccgcg atcaagccga cgccaggcgt gttgaactcg aagatcatcg
     6901 tgccgaagac tggttcgccg tccgccagcg ctcgcctgag ctcgctcggg ttcatggacg
     6961 atttcgcagg taacgcggcg acactccggc tgcctccaac tccggcttgt tgaccgcgtt
     7021 cgccggcatc tcaccgttca tgatcgaacg gattgaggtc gagatgcttt cggcggtccg
     7081 gcgcgcgaag tcgtgggtcc agccggccga gtgcggcgtc aggatgacgt tgtccatctt
     7141 gagcagtggg ctgtcattct cgagcggctc gcgcgcaaaa acgtcgagcg ccgcgccggc
     7201 gatcttccga tgctgaagcg cctcgatcag ggctggctcg tcgacgatcg gcccgcgggc
     7261 ggtgttgacc agatatgcgg tcggcttcat catcgcgatc cgatcgcggt tgatcatgcc
     7321 tcgcgtctcc ggtgtcagcg cgcactggat gatgatgaag tcggagcgcg ccatcagctc
     7381 atcgagttcg acgcgggtga cgggtcctgc gctctggtcc gcgtatgggt cgtagccgat
     7441 cacctcgcaa tccaagaccg aggcaacccg caggatctcg cggccgatgt tgccgagacc
     7501 aatcacgccg agtgtcttgc cgaccagccc ggtgccgatg aactgatgct tgtcggacca
     7561 gagattgccg ctgcgcatgg cccggtcctg gtcgaagatc cggtgcgcga ggcagagcac
     7621 gaaggccatc gcggtctgcg ccatcgagcg ccgcaccccc tccggcgcgt tgacgacgac
     7681 cacgcccgct tcagcgcact cggcgagatt gaccgtgtcg tatccgacac cccagcggca
     7741 gacgacggcg agccggtcgt tgtcgagcag gctcgaggcg tcgcatgagc caccagacat
     7801 cagcagtgcg tcgatatccc tgatcgcgtc gcactggtag gtcccgccgt tgcattggac
     7861 cgtcctgatc tcgacgtcgt cgggcaaatc caggaatgcg gaccgcagcg ccggcaattt
     7921 cggcacccct tccgcgtcgt aaaaatcgga tccgacacca acaacgaact tgcccatctg
     7981 tgctccccgc ttccgatgcg aatcgcttat gttgacgcag ccccgaacgg acacctacta
     8041 tccgaccgcc aatcggtaga ggttccggct gctcgatcgc caggattgaa cgtgcccgtt
     8101 gttcgtattc ccagactagc gtcaatctgc gcggcgattg ttgctctcat tctcgcaatt
     8161 ccggcccttc cggtgcacgg gcagtccccg gaccccgcga cactggatca gatcagctcc
     8221 aagcgttaca tcgtgatcga cgaccagacg ggcgaaatct acgcccagaa ggacgccgat
     8281 acccgcggtg ggatcgccag cctgaccaag gtgttcactg cgatcgtcgc actcgagcat
     8341 gcctcgctgg atacgcaaat caccgcaaac gagtccgacg agttcgactc gacgtccacg
     8401 cggatgaccg gctttgcgcc cgggaccaca tacacggtgc gcgacctgct ctacggcatg
     8461 attctcgagt ccgggaacga cgccgcccat gcccttgccc gcggcgtcgg cgcgcaaccg
     8521 ggtgactccg gcgaggattc tgtcaaccga ttcgtcggct ggatgaacga caaggtcacg
     8581 gcgctcgggc tcacggacac gcatttcgtc aacccgcacg gcctcagcga tcccgaccac
     8641 tactcaaccc cgcgcgacat cgccaccttc atgatgtacg ccgtccagaa cgccgacttc
     8701 atggcgatca tcaccgcgcg caattacacg acgacgacgg gcgccacgct cacctcggtc
     8761 aaccgcggac ccgagttcat cccggactac atcggcggca agaccggcta cgacgatgcg
     8821 accggttact gcctggtcga gatcggccag cgcgacgatg cccaactcgt ctcggtcacg
     8881 atcgacggtg tcgcacccga ggtctggtac gaggaccatg cggtcctgca ggagtacggc
     8941 ttcgctgcca ggtcggaacg catcgcagcg ggccagcccc tcggcgacgt gctcgcctat
     9001 gtcggccaac ccggatcgga gcaggcgcag gtggacgagc cggtcgaaca gcaaacgccg
     9061 ccggccagcg atatcttcgc cggcgtcgaa actggcccgc gaccagtgct tgtgaccggc
     9121 acacccgtcc catttgccgc ggtgaacgac ggtggggaca gtccgtacca caactggttg
     9181 atcggtctgg tcatcgcggc gatcgtaacc gggtccctct ggatgcacgc cggaatgaag
     9241 ctgcgcttcg tcgcccgcag accgatgccc gaacccggcg tggagattga cacgtagccg
     9301 gtgcgttcct atccttgcag caatggcgct gaccaggacg agtatgtcgt cgcgcccaga
     9361 cagaaacgcc gccggatgct gagaggcggc cctgggaaac cgacagaaaa ccacctgcga
     9421 gctgagcggt agacgaccac gatccaaacc gctccggacg ccccgttacc ggcacacgag
     9481 cgatgcgctt ccgcgcgtcg gaaccagggt ggaaccacgt cagcccggcg tccctttgcg
     9541 agggagccgg gctgtttctt ttgctgattg gaggattgat ccgatgtcta tggctgaagt
     9601 gaaagagccg ggttcggacg tcgtcgtcga gatcgagaat gagcaggacc ttgccctggc
     9661 cacgatgcgc cactctgccg cccacctgat ggccgaggcc gtccgggagc tgatcccgga
     9721 ggtcaagttc gcgatcggtc ctgctatcga gaacggcttc tactacgaca tggatctgcc
     9781 gcgaccgctt tcgccggacg atctccaggc gatcgagaac ctgatggccc gccatcgcga
     9841 ggcggacgag ccgttcgtcc ggaccgaagt cagcaaggcc caggcgctcg agacgttcaa
     9901 gaacaacccg tacaaggttg agatcctgaa ggaactgccg gacgactcga caatcacgac
     9961 ctaccagcag ggtacgttcc tcgatctctg caagggacca cacgtcgaat cgacgggcaa
    10021 gatcggcgca ttcaagctgc tttcggttgc cggtgcttat tggcgcggcg acgagaagcg
    10081 gccgatgctg cagagaattt atggcacggc ctggccgacg caagaggagc tggacgacta
    10141 cctcgctcgc atcgaggagg cccagcgccg ggatcaccgc agattgggtc gtgacctcga
    10201 tctcttttct gtaaacgacg agatcggtcc gggcctgatc ctctggcatc cgcgcggcgc
    10261 gatgatccgc taccaggtcg agcagttcga gcagaaggaa cagctcgagc gcggttacca
    10321 gatcgtctac acgccgcaca tcgcgtccga gaagatctac cagaccagcg ggcacctcga
    10381 gacgtaccgg gagaacatgt actccccaat gtcgatcgag gaagtcgact actacctgaa
    10441 gccgatgaac tgccccggcc acatcaagat ctaccagagt cacgtccact cgtaccggga
    10501 cctgccaatc cggtacgcgg aactcggcac ggtctaccgg tacgagcggt caggcaccct
    10561 tcacggaatg ctccgcgtgc gcggcttcac gcaagacgac gctcatatct tctgcacgga
    10621 agagcagatg atcgatgaga tcatcggcgt gatcgacctg gcaacctaca tggccagggt
    10681 cttcgggtac gacttcaagg cctaccttgc gactcgcccc gacaagtggg tcggcgatga
    10741 gcaaacgtgg gcccatgcga cggatgccct caagctcgcg ctcgagcaaa agggcgtgga
    10801 ctacaagatg gatgaaggag gcggagcctt ctatggtccc aagatcgaca tcaagtgggt
    10861 cgacgcgctc ggccgcgagt ggaccggccc aacggtccag ctcgacttca acctgccgca
    10921 gcgcttcgac atcaactaca tcggcgagga tggcgagaag caccgcgccg tcatgatcca
    10981 ccgcacgctg ctcggttcga tggagcgctt cgtcggcggt ctggtcgagc actatgccgg
    11041 tgcctttccg gtgtggctcg ctccaaccca gatcgcgctg atcccgatca cggacgatca
    11101 ggtggagtac tgccagaat
//
LOCUS       DATAOZ010000118         8540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6888408, whole genome shotgun sequence.
ACCESSION   DATAOZ010000118 DATAOZ010000000
VERSION     DATAOZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6888408"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(448..1935)
                     /locus_tag="VHR64_03335"
     CDS             complement(448..1935)
                     /locus_tag="VHR64_03335"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HEX3301887.1"
                     /translation="MIRRLSALVMICALALSGLAGIAPAAAQAGVTVNIRTIDAQTGE
                     SIRSACFVLVDFSNTGCDENGDGLIRYEGVPAGDYLVDQVQPAAGYVTVHDIPITVKA
                     SPSTQTFTIRLDPVEANQAPASNDHVTLYVRTIDAANQFAITDVCYVLQDFSNEGCDD
                     NGDGRVAFEGLKDGQELTIVQTSRPDGYLPVGEFPVTIVNDGGQQTVDVVMSQDRSTT
                     DTIDVSVVPYDAATGGGLTGACVIFLGGSNEGCDDNGDGRISFEGVPVGSYLLRETQA
                     PQGFEPAPDRWVALQVTGRTLYLDHAPTEQVQPADEVNVALVTRDPKTGDLLNGACYI
                     ILDASIEGCDENNDGQVDYEGVVPGTYTIHQTKAPAGHATINDFEAQISEFDPEQSIL
                     VKQAKEQYAPGFRNVSVAIYDVDSGQRVKGEDVCVVLVDFSNEGCDLNGDGQIDFQDV
                     PVGEYTIEATSLPKGYNVYFKDNLVVVEETNPLSIANALLVIVKP"
     gene            complement(2155..2931)
                     /locus_tag="VHR64_03340"
     CDS             complement(2155..2931)
                     /locus_tag="VHR64_03340"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: protein motif:HMM:NF010703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HEX3301888.1"
                     /translation="MRWDPQKYGQFSDERSRPFFDLVAQVRAENPRRVVDLGCGSGEL
                     TATLAERWPDAEVTGVDSSPQMIERGTSLTGAPANLHLQIGDIADWRIEPDVDVMVSS
                     AAFQWVPTHRELIQEWARVASPGTWLAWQVPGNFGAPSHVLMRKLAESAEWSERLAGV
                     LRHNRPVAEPAEYLAMLTREGFAAQAWETTYLHVLQGPDPVLEWVRGTALRPVFAVLD
                     EADARSFEAQYAALLREAYPPAEMGTIFEFRRIFCVGRKE"
     gene            complement(3095..3967)
                     /locus_tag="VHR64_03345"
     CDS             complement(3095..3967)
                     /locus_tag="VHR64_03345"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3301889.1"
                     /translation="MHVHKRITVERPRPQVYAFWRNLTQLPQFMDHLASVQGDTEGVT
                     HWRAKGPGGSVEWDAELVEDKPNELISWRSLEGSDVRNSGAVRFFDAGSGATEVAVDI
                     SYDPPMGRLGNLVASILGESPEQEIGSDLKRFKEIMESGQADISGSSAPTVPADVPTA
                     TAAGQELAASRGETHAATNQGRYETDLNPQKVDNDAQGDELIIGGPMMANPGYSSTGL
                     VGATDPAEAGVIGATRGSVPGSDSDLANDVDPDQLGLRRGHDQNRVDPNDRDLGNPDA
                     PEHDIGYRPSGAPL"
     gene            4396..6243
                     /locus_tag="VHR64_03350"
     CDS             4396..6243
                     /locus_tag="VHR64_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3301890.1"
                     /translation="MPSTHAPVLTSAISSFADLDLRATTNAALRRVNITTPTPIQAGS
                     LPILLAGRDLIGQSRTGSGKTLAFGIPAVQSVDPAIKTVQVLVLTPTRELAVQVGSVL
                     HSLAEGTGIKSVLVYGGRAMGPQKDALRRGAQIVVGTPGRVADLYGQGDLRLDNVRFL
                     VLDEADEMLDRGFGPQVAKILSWTPRERQTALFSATMPEWVNDAARKHLRNPEHVAIV
                     PTQAETTQIDHIAYDVPTGGKMDALKDLLDARGDGQVIVFGRTKHGVKKLAKQLQAAG
                     YPVAALQGNMSQNARDQVMDDFRTGKIAILLATNVAARGIDVTAVDHVINFELPESSE
                     LLQHRIGRTGRMGKTGSAITLLTAEDADKWRQLMRGHGRQIPRQPWTGARRPDAAPRV
                     VAQAPGSRGVEESRSREAGRGGDQARRRDVRADGNLPARSDNGRRKPENRANADSTPP
                     TERPRNRRSSRREQQPVQPAQAQRRSSSISVVSDMIRERRTDHRSNGVSENGNAALRG
                     SFQFDDLIDQEKFSFRNRGVSRHSDGKVGNGSIPANGTGRPFFPVREERPVESAPARQ
                     PEGQHTATCTNCGGTAVLRFAPDLSRPVYCDTCFSERRKSRRRQPTQTA"
     gene            6735..7094
                     /locus_tag="VHR64_03355"
     CDS             6735..7094
                     /locus_tag="VHR64_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301891.1"
                     /translation="MPGPHPPIPESTGPTGFLISRKAAIWVTGIALFVSAMTWLALYE
                     IKVIAEDNPPTQTEIAECKAELDPNSTVDPDVACPPTAHIVWAARHPLRNSIVVLVVL
                     LILGYDMVILNRYLRTR"
     gene            complement(7152..>8540)
                     /locus_tag="VHR64_03360"
     CDS             complement(7152..>8540)
                     /locus_tag="VHR64_03360"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3301892.1"
                     /translation="IRAALAIAIEERDAVRAHRIGALLWNFWHLRGHQQEGRRWYEQI
                     LALPAGEPTKDYAYCLRGAGEIASEQGRFAEAVQLYEEALAIVRPLGEKRLEAQIIGS
                     LANADYGQGNFAEASRLYHEAIAIHDTTDDRRSLATCHNNLGNVSLYLNRLDDAEAEY
                     SRARDLLLELGDRRGALVVLGNIGAVVQYQGNLDRAIQIFQEVLAGMREIGDERGIAS
                     ALSNLGSTFTDLGDLDQAKAYCLQAIEIMERIGLVRMIALTELSVANIEMQRGDNVQA
                     IEWATKSLGNLVACGDVPSSGVLLGMLGDLAFNVGEPVRSARLFGGALAQIEAADAQD
                     AIADPEQYEQTVSRTRNALGTTAYEAEFAAGKVMPLDDLLSVARELREIAISAPGDPE
                     SAAIEQRTGLNAREATALRLFVAGRSNQEIADGIGISLAEAVALVGRVMTRIKVESRA
                     EATAFAFKNGLV"
BASE COUNT         1448 a         2996 c         2565 g         1531 t
ORIGIN      
        1 cttgatgccg agcgcgacct tctcctcctc gctcaacgcg tagtcggcca tatcgaagac
       61 ggcttcgacg ccgtgatgct cttcctcctg gaggtgcccc ggaatctcat cgcccggcac
      121 ctcgcccttc tcgagctcct cttccaggtt gtcgtctttg tccctgtcag tcatatcgtc
      181 tcctcgcgag ccggccggat taagactccg gccacgcggc ctcgaagtcg ctatcaccgt
      241 agaacccgga ctcccgcaat gtcagcgtcc cgactacacg caggtggagg gttgcgggtt
      301 gagggaatga tgtaggggca gatggcatct gccctcacat gtccctcagc gggtttccca
      361 atgcatccat tggtgaccgg tcggtgcatg gcaacgacac cctcggcgag cggcgggcag
      421 atgccatctg cccgacattg ccactcgtta cggtttgacg atgaccagca gcgcattcgc
      481 gatcgagagc ggattcgtct cctccaccac cacaaggttg tccttgaagt agacgttgta
      541 ccccttcggc aggctcgtcg cctcgatcgt gtactcgcca accgggacat cctggaagtc
      601 gatctggccg tcgccgttca ggtcgcagcc ctcgttgctg aagtcgacca gcaccacgca
      661 gacatcctcg cccttgacgc gctgcccgga gtcgacgtcg tagatcgcga ccgagacgtt
      721 gcggaaaccg ggcgcgtact gctcctttgc ctgcttgacg agaatcgact gctcgggatc
      781 gaactcggat atctgcgcct cgaagtcgtt gatcgtggcg tggccggccg gcgccttcgt
      841 ctggtggatc gtgtaggtgc ccggcacgac gccctcgtaa tcgacctggc cgtcgttgtt
      901 ttcgtcgcag ccctcaatac tggcatcgag gatgatgtag cacgcgccgt tcagcagatc
      961 gcccgtcttg ggatcccgcg ttaccagcgc gacgttgact tcgtccgccg gctggacttg
     1021 ctcggtcggc gcgtgatcga ggtagagcgt ccgtccggtc acttgcagcg cgacccagcg
     1081 gtccggcgcc ggttcaaagc cctggggcgc ctgcgtctcg cggagcaggt aactgccgac
     1141 cggcacgccc tcgaacgaga tacggccgtc tccgttgtcg tcgcacccct cgttgctgcc
     1201 gccgaggaag atcacgcacg cgcccgtcaa tccgccgccg gtcgctgcgt cgtacggcac
     1261 gaccgagaca tcgatcgtgt cagtcgtcga gcggtcctgg ctcatcacga cgtcgaccgt
     1321 ctgctggccg ccgtcgttga cgatcgtgac cgggaattct cccaccggca ggtagccatc
     1381 cgggcggctg gtctgcacga ttgtgagctc ctggccatcc ttcagccctt cgaaggcgac
     1441 gcggccatcg ccgttgtcgt cgcagccctc attgctgaaa tcctgcagca cgtagcagac
     1501 atcggtgatc gcgaactggt tggccgcatc gatcgtgcgg acgtagagcg tcacgtgatc
     1561 gttcgaagca ggcgcctgat tggcctcgac agggtcaagc cgaatggtga acgtctgggt
     1621 agacgggctg gccttgaccg tgatcgggat gtcatgaacg gtgacatacc cggccgcggg
     1681 ttggacctga tcgacgaggt aatcgcccgc cgggacgcct tcgtatcgga tcaggccatc
     1741 gccgttctcg tcgcagccgg tgttgctgaa atcgacgagt acgaaacagg ccgatcggat
     1801 cgactccccg gtctgggcat cgatcgtacg gatattgacg gtcacgcccg cctgagcggc
     1861 cgccggcgcg atccccgcca acccggagag cgccagcgcg cagatcatga ccaatgcaga
     1921 aagccgtcgg atcatcgttc ccccttccag tgcgccggtt cgcggacacg ccaaccgagc
     1981 accaccctga ctggccctat caaccccaac gcctcttacc ccaatcaagt ttctggtctg
     2041 tcgtctttgg tctctcgtct ttcgttttcg gttttccgtt ttccgagatc catccaccaa
     2101 caaccaacaa ccatcaaccc aactgctcgc ctcctcgact gctcgcctgc ttgcctattc
     2161 ctttcgtccc acacagaaga tgcggcggaa ctcgaagatc gtgcccatct cggcgggcgg
     2221 gtaggcctcg cggagcaatg ccgcgtactg tgcctcgaag gagcgggcgt cggcttcgtc
     2281 gaggacggcg aagaccggcc ggagcgccgt tccccgcacc cactcgagga ccggatccgg
     2341 cccctgcagc acgtgcaaat aggtcgtctc ccacgcctgc gccgcgaatc cctcgcgggt
     2401 cagcatcgcc aggtactcgg ccggctccgc gaccggccgg ttatgacgca gcaccccggc
     2461 cagcctttcg gaccactcgg ccgactccgc cagcttccgc atcagcacgt gcgacggagc
     2521 cccgaagttg cccggcacct gccaggccag ccatgtcccg ggtgaggcca cccgcgccca
     2581 ctcctggatc aactcccggt gcgttggcac ccactggaaa gccgcgctgc tgaccatcac
     2641 gtcgacgtcg ggctcgatcc gccagtccgc aatgtcgccg atctgaaggt gcaggttggc
     2701 cggcgcaccg gtcaacgaag ttccccgctc gatcatctgc ggcgagctgt cgacgccggt
     2761 cacttcggca tcgggccaac gctcggccag cgtcgccgtc aattcacccg acccgcagcc
     2821 gagatcgacg acccggcgcg ggttctcggc ccgaacctgc gcgacgagat cgaagaacgg
     2881 gcggctccgc tcgtcgctga actgaccata cttctgcgga tcccaacgca tccctctctc
     2941 cccgacagca acgaccgtac gcaaacccgt gcaagaaacc atgatccggt caccacggtc
     3001 aacccccggg gtcacgcagg caaaacggcg ggcaactggt cccgccgttc tgcgatttcg
     3061 atggattcca ggccgctatt tggcaagctg tccgttagag cggcgcgccc gaggggcggt
     3121 agccgatgtc gtgctccggt gcatccgggt tgccgaggtc gcggtcgttc ggatcgaccc
     3181 tgttctgatc gtgtccgcgt cgcagaccga gctgatcggg atcgacatca ttggcgaggt
     3241 ccgaatcgga gcccggcaca gacccgcgag tcgcgccgat caccccggct tcggccggat
     3301 cagtggctcc gacgaggccg gtgctcgaat agccggggtt ggccatcatc ggaccgccga
     3361 tgatcagttc atcaccctgg gcatcgttat cgaccttttg cgggttgagg tcagtctcgt
     3421 accggccctg gttcgtcgct gcatgcgtct ctccgcggga ggcggccaac tcctgcccgg
     3481 cagccgtagc cgtcggcaca tcggctggga cggtcggcgc cgaagagccg gaaatatctg
     3541 cctgaccgct ctccatgatc tccttgaatc gtttcagatc ggagccgatc tcctgctcgg
     3601 gcgattcccc gaggatgctg gcgacgaggt tgccgaggcg gcccattggc gggtcgtacg
     3661 agatgtcgac ggctacttcg gtcgcacccg acccagcatc gaagaaacgg acggcgcccg
     3721 agttgcggac gtcggatcct tcgagcgagc gccacgagat cagctcattc ggcttgtctt
     3781 cgaccaactc cgcgtcccat tcgacgctgc cgccaggtcc cttggcccgc caatgggtga
     3841 cgccttccgt gtcgccctgg accgaggcga ggtggtccat gaattgtggg agctgggtca
     3901 ggttgcgcca gaaggcatag acctgcggtc gcggtcgttc gacggtaatt cgcttatgga
     3961 catgcatggt gcgcatccct ccttcacagg tttgtggccg acctgcctca tcgcagaccg
     4021 gccaccccaa ccctaccccg cagcagcggc gccgaggtcc ccacaactac caatcagcga
     4081 tacatgcgct acatgccggg tggagggtga agggaacgaa aacccgtagc gccgcgcctc
     4141 cgcgcctccg cgcctcaact gctcgcctgc tcgcctgctc gcctgctcga catctcgact
     4201 gatttatgtc atactcgtaa tcacgaatgc gaccacgcgg gatacgcccg tgtgcgccac
     4261 gctcagctcc cgtacctgtg cagcgtgcag cagggggatg actgggcggc atgatccgct
     4321 tccagtcggc tccccgtgat ctgacaccgt tcgccatcat ggcgtttctc tgcacgcgta
     4381 ctcaggagct tttttgtgcc ttcaacccat gcacccgttc ttacgtcggc gatctcgtcg
     4441 ttcgctgatc tcgatctgcg cgcgacgacc aacgccgcgc tgcgccgggt caacatcacg
     4501 actccgacgc cgatccaggc cggcagccta ccgatcctgc tcgcgggacg cgacctcatc
     4561 ggccagtccc ggaccggctc cggcaagacc ctcgccttcg gcattcccgc cgtccagtcg
     4621 gtcgatcccg ccatcaagac ggtccaggtc ctcgtcctga ccccgactcg cgagctcgcg
     4681 gtccaggtcg gcagcgtcct tcactcgctg gccgaaggaa ccggcatcaa gagcgtgctc
     4741 gtctacggag gccgcgcgat gggtccgcag aaggacgcgc tccgccgtgg cgcccagatc
     4801 gtcgtcggca cgccgggtcg cgtcgccgat ctttacggtc agggcgatct gcgcctcgac
     4861 aacgtccgct tcctcgtcct cgacgaggcc gacgagatgc tcgatcgcgg tttcggtccg
     4921 caggtcgcca agatcctgtc ctggaccccg cgcgagcgcc agaccgccct tttctcagcc
     4981 acgatgccgg agtgggtcaa cgacgccgcc cgcaagcacc tgcgcaaccc cgagcacgtc
     5041 gcgatcgttc caactcaggc cgaaacgacc cagatcgacc acatcgccta cgacgtcccg
     5101 accggcggca agatggacgc gctcaaggat ctgctcgatg cccgcggcga tggccaggtg
     5161 atcgtcttcg ggcgcaccaa gcacggcgtc aagaagctgg ccaagcagtt gcaggcggcc
     5221 ggctatccgg tggccgcgct ccagggcaac atgtcccaga acgcgcgcga ccaggtgatg
     5281 gacgatttcc ggaccggcaa gatcgcgatc ctgctcgcca ccaatgtcgc cgcccgcggt
     5341 atcgacgtca ctgccgtcga ccatgtcatc aacttcgagc tgccggagtc gtccgagttg
     5401 ctccagcacc ggattggccg caccggccgc atgggcaaga ccggctcggc gatcacgttg
     5461 ctgaccgctg aggacgccga caagtggcgc cagctcatgc gcggtcatgg ccgccagatc
     5521 ccgcgtcaac cctggaccgg tgcacgccgc ccggatgccg cgccgcgggt cgtggcgcaa
     5581 gcgccaggga gtcgaggagt cgaggagtcg aggagtcgag aagcagggcg cggaggcgat
     5641 caggcgcgga ggcgcgatgt aagggcagat ggcaatctgc ccgcgcggtc ggacaacggg
     5701 cgacggaaac ccgaaaaccg agctaacgcc gattcgacgc ctccgaccga acgcccacgc
     5761 aaccgccggt cttcgcgtcg tgagcagcag ccggtccaac cggcccaggc gcagcgccgg
     5821 tcgtcgtcga tctcagtcgt ctcggacatg attcgcgaac gccgcactga ccaccggtcg
     5881 aacggcgtgt ctgaaaacgg caacgcagct ctgcgcggct cattccagtt tgacgacctg
     5941 atcgaccagg agaagttctc gttccgcaat cgcggcgtct ctcgccattc cgatggcaag
     6001 gttgggaacg gctcgatccc ggcgaacggc accggtcgac cattcttccc ggtccgcgag
     6061 gagcggccgg tcgaatcggc tcctgccagg cagccggagg gccagcacac tgccacctgc
     6121 accaactgcg gcggcacggc cgtgctccgg ttcgccccgg acctcagccg cccggtctac
     6181 tgcgacacct gcttcagcga gcgccgcaaa tcccgccgcc gccaaccaac ccaaaccgca
     6241 taaccagcga tcagcccggg ccgaaatgcc cgggctgatt cattcagtca gcattgccgg
     6301 cgcacgaagt ccaacgtagc gcggggatac cacgccccgc atcgatgatc cctacgcatg
     6361 gttcatagag acggttcgtg atcagggttg gttcttgcat tggatgttgc ggtcgaatcc
     6421 gggcggggcg tggtaacccc gcactacgac tttgacgcgc ccactacccg agtccgctcg
     6481 ctattcatga cggtcccccg tggccggaat ggggtcgacg gcccgaccgg ttgatggagc
     6541 cggcgtgcat ctttcaccgg gtctcccgac accgggacgg cgcagaggcg gaccgtgcta
     6601 cgccaggaac gcaggccagg ttcggtcggc atcgataccc gatctctcga cactccacgc
     6661 tcaacccatc gggtaggatt gatgaccggc cggtccgttt ggcgatcggt atctcccatc
     6721 caccgggaaa acgcgtgcca ggtccgcatc caccgatccc tgagtcgacc ggcccaactg
     6781 gcttcctgat cagccgcaaa gccgcaatct gggtcacggg catcgctctc ttcgtctccg
     6841 cgatgacctg gctggcgctc tacgagatca aggtgatcgc cgaggacaat ccgccgaccc
     6901 aaaccgagat cgccgagtgc aaggccgagc ttgacccgaa cagcacggtc gacccggatg
     6961 tcgcctgccc acccacggcg cacatcgtct gggcagcccg tcacccgctc cgcaactcaa
     7021 tcgtcgtcct cgtcgtcctg ctcatcctcg gctacgacat ggtcatcctg aaccgctacc
     7081 tgcgcacgcg gtgagagtgg tcaggggtca gtaagtgcgt agccccaacg gctcgccttc
     7141 gcgccttcgc ctcataccag gccattcttg aaggcgaatg ccgtcgcttc ggcgcgcgat
     7201 tccaccttga tcctggtcat gacccggccg accagcgcga ccgcctccgc caggctgatg
     7261 ccgatcccgt ccgcgatctc ctggttcgac cgaccggcca cgaagagccg caacgccgtt
     7321 gcttcccgcg cgttcagccc cgtccgctgc tcgatcgcgg ccgactcggg atcgcccgga
     7381 gctgagatcg cgatctctcg cagctcgcgg gccacgctca gcagatcgtc aagcggcatt
     7441 accttgccgg ccgcgaactc cgcttcgtag gcggtcgtgc caagcgcgtt gcgagtcctg
     7501 ctcaccgtct gctcgtactg ctccggatcc gcgatcgcgt cctgcgcgtc ggccgcttca
     7561 atctgggcca gcgcaccacc gaacaaccgc gcgctccgga ccggctcgcc gacgttgaac
     7621 gcgagatcgc cgagcatgcc aagcagcacg ccgctggatg gcacatcccc gcacgccacc
     7681 aggttgccca gcgacttggt agcccattcg atcgcctgaa cgttgtcccc ccgttgcatc
     7741 tcgatattcg cgacggacaa ctcagtcagc gcgatcatcc ggaccagacc gatccgctcc
     7801 atgatctcga tcgcctgcag gcaatacgcc ttcgcctggt ccagatcgcc aagatcggtg
     7861 aaggtcgaac cgaggttcga gagggcgctg gcgattcccc gctcatcgcc gatctctcgc
     7921 atgccggcca gcacctcttg gaagatctgg atagctcggt cgagattgcc ctggtattgg
     7981 accaccgcgc cgatgttgcc gaggacgacc agtgcaccgc gccgatcccc aagctccagg
     8041 aggagatccc gcgctcggct gtactcggct tcggcgtcgt cgaggcggtt caggtagaga
     8101 ctgacattgc ccaggttgtt gtggcaggtg gcaaggctcc gccgatcatc cgtggtgtcg
     8161 tggatggcaa tcgcctcgtg atacaggcgc gacgcctcag cgaagttccc ttgcccatag
     8221 tcggcattgg cgagcgaccc gatgatctgg gcctccaggc gcttctcgcc gagtggccgc
     8281 acgatcgcca gggcctcttc atagagctgc accgcctcgg cgaagcggcc ctgctctgac
     8341 gcgatctcgc ccgctccgcg caggcaatac gcgtagtcct tcgtcggctc tccggccggc
     8401 agcgccagga tctgctcgta ccagcgccgg ccctcctgct ggtgaccgcg cagatgccag
     8461 aagttccaga gcaacgcgcc gatccggtgg gcgcgtacgg cgtcccgctc ctcgatcgcg
     8521 atggcgagcg cggcacggat
//
LOCUS       DATAOZ010000119         7221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1629239, whole genome shotgun sequence.
ACCESSION   DATAOZ010000119 DATAOZ010000000
VERSION     DATAOZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629239"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..404)
                     /locus_tag="VHR64_03365"
     CDS             complement(153..404)
                     /locus_tag="VHR64_03365"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX3301893.1"
                     /translation="MIEGNAAEVKTLDVREITPRERHPLIFQTIDDLKPGTALRLVND
                     HDPKPLYYQLMAERPGKVDWTYLEEGPETWQVRITKKAS"
     gene            complement(505..1818)
                     /locus_tag="VHR64_03370"
     CDS             complement(505..1818)
                     /locus_tag="VHR64_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301894.1"
                     /translation="MNGLNSANAPALDLPARFMALSITALAIIGVSAPWTLPLMQGSF
                     GNFSLLAFVHLWTLGFVGAMIIGASYQLVPVALQVPLSSPRAGRISFWFYGAGLAMFL
                     AGLITTRVALIGIGGTSLAIAFLIYIGVIAATWIQTPDRDVVAWHIALGLAGAFVGMA
                     LGFSLALNKGNGMLGSRLLEVLAAHITLMLGGWIAITFTGVAYRLIGMFTLSERHFIP
                     WLAWTELALCAMGSWLLAASFIFDLAPVVGQAAAVALLAGLVCFASQIARLYRKRMRR
                     GIDVHIPFAALAALSGIASTGLLVFGLAKHEGPGSSVWVVVVWMALMGMAASAIQGFF
                     YKIATFLVWLKRYAPVAGRERVPRLEELYNRRLALTGFAMWSASILGGAIVLVGNQDV
                     LRPVGAAMLAGLTCFLINVAMIARHWIGGIRPRVLRVATRHEPAH"
     gene            complement(1815..2654)
                     /locus_tag="VHR64_03375"
     CDS             complement(1815..2654)
                     /locus_tag="VHR64_03375"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX3301895.1"
                     /translation="MSSKQPILASERVSDILNRYPTLVDTLIDASPAFRNLRNPIMRR
                     VQSRLVTVSQAAGIAGLSPAELTRTLNVAAGLTAPSETDFTEPGQPTSAEPLLDDVDV
                     SIPPIAAEIDVRTIMARGEEPFKAIMSVASSVPAGAAFRLIASFEPLPLYDVLAKQGF
                     THRSHEIGDGAWEVQFIRAAEIRHRSSKSARKAGSEDDWPAEITTEVTIDVSELVPPE
                     PMIKILEALEQLEPGGRLLVHHVRRPIHLYDRLDEMGYRHQTRDLAPGNVEVMIEKPV
                     GAS"
     gene            complement(2688..2999)
                     /locus_tag="VHR64_03380"
     CDS             complement(2688..2999)
                     /locus_tag="VHR64_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX3301896.1"
                     /translation="MPSTTLSPGSLLDSLMQVIDPEVGINIVDLGLVYAVHLTDTEAT
                     VEMTLTTPGCPLHASIEAAVKRALSSNHPELTSVTVQIVWDPRWNVDFITAEGRRQLG
                     W"
     gene            3207..5687
                     /locus_tag="VHR64_03385"
     CDS             3207..5687
                     /locus_tag="VHR64_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX3301897.1"
                     /translation="MSPKEIASVVDLMTLEEQVSLLAGAGFWRTGAIERLGVPSIKVS
                     DGPNGARGEGAFSSGVKAASFPVGISLASTWDPDLVSEIGAAIAQEAKTKRAQVVLAP
                     TVNIHRSPLGGRNFESFSEDPWLAGILGAAYVSGMQSQGIAATVKHFTGNESEFERMS
                     INSVIGERALREIYLRPFEIAVRQARPWAVMAAYNRLNGTFCSDNHRLLTEILRDEWG
                     FDGFVMSDWTGTRSTAAAINAGLDLEMPGPAKQRGEKLVAAARTGEAHRSAIAASAGQ
                     IVRLAERVGASISSSAEEQAVDDPRHRELIRRAGASGTVLLTNNGVLPLDRRAITSIA
                     VVGPNAAVAQIMGGGSSQINAHYRVSPLDGIRAAVPDGVDVRTAPGCDNRRLLPAVQA
                     GVHIEYFANDQFEGEPAHSEDRAGSDIMWLGHIAPGVDIHHFSARLTTVFEPDRADRY
                     EVGVVSSGPVRVLINDQVVVDQWDTWQAGGEYFGLASREYRGSIDLKAGTSYQVIIEN
                     RTPDQGPEVGLKALRLGIARPTGDDELDEAVQLAAGADVAIVCVGLNGEWDTEGMDRP
                     DMDLPGRQNHLVERVADANPNTIVVLQSGGPLTLQWVDDVAAVMQAWYPGQECGNAIA
                     DLLFGDVNPSGKLPMTWPRSLANNPTTLSFPGERGVAEYREGLFVGYRFYEAMETPPL
                     FAFGHGLSYSTFAYESIRLSASELKPGDHLTVDVEVRNVSDRAGAEIVQLFVRDPESV
                     LRRPRKELKGFARVELAAGSSGSSRLTLDMRSLAYFDDQRNAWVADAGEFEIMVGPSS
                     DDIRLCASVTLANEWVESAQDAWRSTVI"
     gene            complement(5911..7134)
                     /locus_tag="VHR64_03390"
     CDS             complement(5911..7134)
                     /locus_tag="VHR64_03390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3301898.1"
                     /translation="MATSLPSSADVVVIGGGIVGTSTAWHLAQQKAGRVVLLEKATIA
                     AGASGWTGALLRRHYTNLPEATLAHQSHLVFRNWAEIVGGSCGYEPQGLIVTVDQSPE
                     VAQNVELLSRNVEFQQSLGIETEVVTAEQLKALQPWCFVDDIQTAAYEPISGYADAPL
                     ATRSMADAAERAGATILEWVETACIRTDGTRVIGVETDQGTIATNTVVLAAGPWSTKL
                     AAAAGVRLPIETIRVQIAIVHRPRELTEAHFVFLDMAAGCFMRPWAPGRSLLGVAGGD
                     QHDAVDPDNFALGNDPAYPDQAKAAAAKRIPAMSRAQYLHGHAGLYDMSPDTHPIIGL
                     AGPEGLIVACGFSGAGFKKGPAVGQCIAELITEGQSNLVDLAPFRIERFDAPGWDRPW
                     SNTEYAFTSDFGHKL"
BASE COUNT         1386 a         2211 c         2318 g         1306 t
ORIGIN      
        1 ccgggcaagg tcgactggac ctatctcgaa gaaggcccgg agacctggca ggtccggatt
       61 accaagaaag ccagctaacc ttcatttcct cacgttacgg ggggcctgcc gcacacctgc
      121 gacgggcccc ccgtaacgtg aggaaatgaa ggttagctgg ctttcttggt aatccggacc
      181 tgccaggtct ccgggccttc ttcgagatag gtccagtcga ccttgcccgg tcgctccgcc
      241 attaactggt agtagagcgg cttcggatcg tggtcgttga ccagtcgcag ggctgtcccc
      301 ggcttcaaat cgtcgatcgt ctggaagatc agcggatgtc gttctcgtgg agtgatctcc
      361 cgtacatcca acgtcttgac ttccgccgca ttcccctcga tcacgtggac ctccttcgat
      421 atggcgcgtt gccgcgcact cttgtccgct cccgaatgag ccgacatcac acccaaatac
      481 gtgcgtccag accggtcgat tcggtcagtg cgccggttca tgccgcgtag ccacgcgtag
      541 taccctcggc ctgattccac cgatccaatg gcgagcgatc attgcgacgt tgatcaggaa
      601 gcaggtcagc cctgcgagca tggcggcgcc cactggacgc agtacgtcct gattgccgac
      661 cagcacaatc gctccgccga ggatcgatgc gctccacatt gcgaatccag ttagggccag
      721 acgccggttg tagagctctt cgagtcgggg gacccgttcc cgcccggcaa ccggcgcata
      781 ccgcttcagc caaaccagga acgtggcgat cttgtagaag aagccctgaa ttgcgctggc
      841 agccatgccc atgagcgcca tccagacgac gacaacccaa accgacgaac ctggtccctc
      901 gtgctttgca aggccgaaga ccagcaatcc ggtggacgct atgcctgaca acgccgcaag
      961 ggccgcgaac gggatgtgaa catcaatccc gcgccgcatt cgcttccggt aaaggcgggc
     1021 gatttgactg gcgaagcaga ccagaccggc cagaagcgcc accgctgcgg cttggccaac
     1081 aactggcgca agatcgaaga tgaagctggc agcgagcagc cacgaaccca ttgcacaaag
     1141 cgcgagttcg gtccaggcga gccacggtat gaagtgccgc tcggagaggg tgaacatgcc
     1201 gatcagccgg tatgcaacgc cagtgaaggt gattgcgatc cagccgccga gcatcagcgt
     1261 gatatgggcg gccagcacct cgagcaagcg cgaacccagc atgccgttgc ccttgttcag
     1321 ggccagcgag aacccgagcg ccatgcccac gaacgctcca gcaaggccca gggcgatgtg
     1381 ccaggcaaca acatcccgat cgggagtttg aatccacgta gcggctatca cgccgatgta
     1441 gatcagaaag gcgattgcca atgacgttcc gccaatgccg ataagcgcga cccgggtcgt
     1501 gatcaatcca gccaggaaca tggcgagtcc ggcgccgtag aaccagaatg agattcggcc
     1561 ggcgcgcggc gacgagaggg gaacttgcag agcgaccggc accaactggt aactggcgcc
     1621 gatgatcatt gctcccacga atccaagcgt ccagagatgc acgaaggcaa ggagcgaaaa
     1681 attgccgaac gatccctgca tcagcggcaa ggtccacgga gccgacactc caatgatcgc
     1741 cagcgcagtg atgctcaacg ccatgaatcg agcgggcaag tcaagggccg gcgcgttcgc
     1801 ggagttcagc ccgttcacga ggctccgacc ggtttctcga tcatcacctc gacattgcct
     1861 ggagcaagat cgcgggtctg gtgccggtac cccatctcat cgagccggtc atagaggtga
     1921 ataggacggc gcacatggtg gaccagcagt cggccccccg gctcaagctg ctcgagcgcc
     1981 tcgaggatct tgatcatcgg ctccggaggc acgagctcgc tgacatcgat cgtgacttcg
     2041 gtcgtgattt cggccggcca gtcatcctca ctgcccgctt tcctcgcaga ctttgacgat
     2101 cgatgccgga tctccgccgc gcggatgaac tgcacctccc aggctccgtc gccgatctcg
     2161 tgcgacctgt gcgtgaatcc ctgttttgcg agcacgtcat agagtggtag cggttcgaag
     2221 ctggcaatca ggcggaacgc cgcgccggcc gggacactgc tcgccacgct catgattgcc
     2281 ttgaacggct cttcgccacg cgccatgatc gttcgcacgt cgatttccgc agcgattggg
     2341 ggtatggaaa cgtcaacgtc atcgagcaat ggctccgcac ttgttggttg accgggttcg
     2401 gtgaagtcgg tctcgctggg cgcggtcaat cccgcggcga cgttgagcgt acgtgtcagc
     2461 tcggcagggc tcagaccggc tattccggcc gcttgggaga cggtgaccag ccggctctgg
     2521 actcgccgca tgatcgggtt gcgcagattc cggaaggcag gtgaggcatc gatcagcgtg
     2581 tcgaccaggg tcgggtaccg gttcaggatg tcgctgaccc gctcactggc gagaatgggt
     2641 tgcttcgatg acatggtgat cctccccgtg gatgacgaaa aacgcggtta ccagccgagc
     2701 tgccgccgac cctccgcagt gatgaaatcg acgttccagc gcgggtccca gacgatttgg
     2761 acggtgacgg aggtcagttc agggtgattg ctcgatagcg cccgcttgac ggccgcttcg
     2821 atcgaggcat gaagcggaca tcccggcgtg gtgagcgtca tctcgacggt cgcttccgtg
     2881 tcggtgaggt ggaccgcata gaccaggccg agatcgacga tgttgattcc gacctcggga
     2941 tcgatgacct gcatgaggga atcgaggagg cttccgggcg agagcgtggt tgacggcatt
     3001 ggtgcggccc agtgagtatt cggattatat ccgacttgtg cagtcggatt atcttgatgg
     3061 tacgaatcgt ggagcgataa atcaatctgt gctggcgttt gattgagccg gcgtgcgcga
     3121 tttcactggg ctggctgcgt gccgcattcg gtgagaacta gactgcaagc aacgtcgcgc
     3181 attcgatgag gcatgaggga agacgcatga gcccgaaaga gatcgcctcg gtggtggacc
     3241 tgatgacgct tgaggagcag gtgtcactgc tggccggggc cggtttttgg cgaactggcg
     3301 caatcgaacg gctcggcgtg ccgtcgataa aggtcagcga cggaccgaat ggcgctcgcg
     3361 gcgaaggcgc attttcaagc ggcgtcaagg cggccagctt cccagtcggc atctcgctcg
     3421 cgtcgacgtg ggatccggat ctcgtgtcgg agatcggggc ggcgatcgcg caggaagcga
     3481 agacgaagcg cgctcaggtc gtgctggcgc cgaccgtcaa tattcaccgc tcgccgctcg
     3541 gtggccgcaa tttcgagagc ttttccgagg atccgtggct cgccggaatc ctgggtgctg
     3601 cgtacgtctc tggtatgcag agtcagggca tcgcggccac ggtcaagcac ttcaccggca
     3661 acgaatccga gttcgagcgc atgtcgatca actccgtgat cggcgagcgg gcactccgcg
     3721 agatctatct gcggccgttc gagattgcgg tgcgacaggc caggccgtgg gcggtgatgg
     3781 ctgcctacaa ccgcctcaac gggacgtttt gcagcgacaa tcaccggctg ctgaccgaga
     3841 ttctgcgcga cgaatgggga ttcgacggtt tcgtcatgtc ggactggacc ggcacccgat
     3901 ccaccgccgc ggcaatcaac gcggggctcg atctcgagat gcccggtcca gccaagcaac
     3961 ggggcgagaa actcgttgcc gcggccagga cgggcgaggc ccatcggtcg gcgatcgcgg
     4021 cgagcgcggg tcagatcgtg aggctcgctg aacgagtcgg cgcgtccatc tccagtagtg
     4081 ctgaagaaca ggcagtcgac gatccgcgtc accgtgagct gattcgcagg gccggggcca
     4141 gcggcaccgt gctcctcacg aataatggcg tactgccgct cgatcgccgg gccatcacgt
     4201 cgatcgcagt ggtcggcccg aatgcggcag tcgcccagat catgggcgga ggcagctcgc
     4261 agatcaatgc ccactaccgt gtcagcccac tcgatggcat ccgcgcagcc gtgccggacg
     4321 gagtcgacgt gcgaaccgcg ccgggctgcg acaaccgccg ccttcttccc gcggtccagg
     4381 ccggggttca catcgaatac ttcgccaacg accaattcga gggcgagcct gcgcattctg
     4441 aggatcgggc cggctccgac atcatgtggc tcggccacat cgccccaggg gtcgatattc
     4501 accacttctc ggccaggttg acgaccgtat tcgagcccga tcgcgccgac aggtacgaag
     4561 ttggcgtggt cagctccggc cctgtccggg tgctgatcaa cgaccaggtg gtcgttgacc
     4621 agtgggacac gtggcaggca ggcggcgagt acttcggcct cgccagccga gaatatcgag
     4681 gatcaatcga tctcaaggct gggacttcat atcaggtgat catcgaaaat cggactcctg
     4741 accagggccc tgaggtcggg ctgaaggcgt tgcgacttgg catcgcccga ccgaccggcg
     4801 acgacgaatt ggacgaagcc gtccagcttg cggccggcgc cgatgtcgca attgtctgcg
     4861 tcggactgaa cggcgaatgg gacaccgagg gaatggaccg gcctgacatg gacctgcccg
     4921 gtcgccaaaa ccacctggtg gagcgggtgg ccgacgcgaa tccgaatacg atcgtcgtcc
     4981 tccagagcgg cggacccttg acccttcagt gggttgatga cgtcgccgcc gtgatgcagg
     5041 cgtggtaccc gggtcaggag tgcggaaatg cgatcgccga tctcctcttc ggcgatgtta
     5101 atccgtccgg caagctgccg atgacctggc cgcgatcact ggcgaacaac ccgacgaccc
     5161 tgtcatttcc cggagaacgc ggagttgccg agtaccgcga aggcttattc gttgggtacc
     5221 gattctacga agcaatggag acgccgccgc tcttcgcgtt cggccacgga ctctcgtaca
     5281 gcacgttcgc gtacgaatcg attcgtctct ccgcttcgga gctcaagcct ggcgatcact
     5341 tgacagtgga cgtcgaggtt cgcaacgtca gtgatcgggc tggagctgag atcgtccaac
     5401 tctttgtgcg cgatccagaa tcggtcctca ggcggcctcg caaggaactc aaggggttcg
     5461 cccgggtgga gctggcggcc ggatcgtctg gctcatcgcg gttgacgctc gacatgcgtt
     5521 cactcgcgta cttcgacgat cagcgcaacg cctgggtcgc cgacgcgggc gagttcgaga
     5581 tcatggtcgg accgtcgagc gatgacattc gcctttgcgc atcggtcacg ctggccaacg
     5641 aatgggtcga atccgcccaa gacgcgtggc gatcgaccgt gatttaaggc ggcgtgcata
     5701 cctggcgacc ggacccgatg acggccgatg cgatgacgga accatcttgg tcaaccgctc
     5761 gccgcttgca accgtgcgag gagatgcgcc gagcggggcg tgttcctctc tccgactctc
     5821 tcctcaagga aaaagggcat ctcagcgcga cgtcggttaa acgttcaatc gattcgaaaa
     5881 ccgcgttagg ggcaaaacct ggccgtgtac ttacaacttg tggccgaaat cgctggtgaa
     5941 ggcgtactcg gtgttgctcc aggggcgatc ccatcccggc gcatcgaatc gctcgatccg
     6001 gaatggcgcg aggtcaacca gattcgattg accctcggtg atcagctcgg caatgcattg
     6061 gccgacggcc ggccccttct tgaaaccagc cccactgaac ccgcaggcca cgatcaatcc
     6121 ctccggtcca gccagtccga tgatcgggtg cgtatcgggc gacatgtcgt agaggccggc
     6181 gtggccatgc aggtattgcg cacggctcat cgccgggatc cgtttggccg ctgccgcctt
     6241 cgcctggtcg gggtaagccg ggtcattgcc gagcgcgaag ttgtcggggt cgaccgcgtc
     6301 gtgctgatcg ccgccagcca cgccgagcag cgaacgaccg ggcgcccacg gacgcatgaa
     6361 gcagccagcg gccatatcga ggaacacgaa gtgcgcctcg gtgagctcgc gcggtcgatg
     6421 gacgatcgcg atctggacgc gaatcgtctc gatcggcagt cgcacaccgg ccgccgcggc
     6481 gagcttggtt gaccacggac cagcagccag cacgacggta ttcgtcgcga tcgtgccttg
     6541 gtcggtctcg acgccgatca ctcgcgtgcc gtcggttcgg atgcaggcgg tttcgaccca
     6601 ttcgaggatg gtcgcgccgg cgcgttccgc cgcgtcggcc atcgagcgcg tcgcgagtgg
     6661 cgcgtcggca tagccgctga tcggctcata cgcggccgtt tgaatgtcgt cgacgaagca
     6721 ccagggctgg agcgccttga gctgctctgc agtgacgacc tcggtctcga tgccgagcga
     6781 ttgctggaac tcgacattcc gggagagcag ctcgacgttc tgggccacct ccgggctctg
     6841 atcgactgtg acgatcaggc cctgtggctc atacccgcag gagccaccga cgatctcagc
     6901 ccagttccgg aaaaccaggt gggattgatg ggccagcgtg gcctcgggca ggttcgtgta
     6961 gtggcggcgc agaagcgcgc cggtccagcc agaagcgcct gccgcgattg tcgccttctc
     7021 cagcaatacc acgcgacccg ccttttgctg cgccaggtgc caggcggtcg atgtgcccac
     7081 gatgccgcca ccgatgacga cgacatcggc tgaggaagga agcgaggttg ccatcggaac
     7141 tccagaatgc gcgtacgtga cgtggggaat catacgggag cctcgggacg agaacaggcg
     7201 gggattcgtg aaacggcccc g
//
LOCUS       DATAOZ010000120         1743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8731133, whole genome shotgun sequence.
ACCESSION   DATAOZ010000120 DATAOZ010000000
VERSION     DATAOZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8731133"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VHR64_03395"
     CDS             <1..622
                     /locus_tag="VHR64_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301899.1"
                     /translation="SGDGAQLAPGTGPDGSQAVTLQSAGSANQPGAASYIRRDLPEGE
                     STLHVAFDLDARELDPGATRLATFTSADSSAIAALYLLPDGTLGVRFGESDSLAPVGS
                     LDLSIWNRIEIGISAGPTGGDMTVWVNGQFSGSATTAAVTAEPRSILIGGWATDRTYD
                     LLVDNVAIDRGCLSGCPEAPAPTATTTPEPAQDVPVAPEPGTPVSG"
     gene            689..>1743
                     /locus_tag="VHR64_03400"
     CDS             689..>1743
                     /locus_tag="VHR64_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301900.1"
                     /translation="MTESALPRWDLTGIFPNPISGEYLEAVAKLRAGIEQMTGLFDEF
                     GVGLDSGAHPQTEPGRIISTWNEIGDLADLLDNYLYCWTSTDSNDAAAASAYGELDMI
                     TSPMRALRSRLTTWLGTLNPDSSNDPVLANHRYQVQQAGIGAKHVMAPELEELAGALQ
                     ATGGQAWVRSRDESDAGLTTTITIDGETRTTSLTELDTLLSSPDREKRRAAAEAMDVA
                     REKAAPQFAASLNAIKGEALTLSRRRKWGSPLDWSLFISAIDREILDAMFAAAESVHG
                     ELHRYLQTKARLLGIEKMAHYDLSAPISKDQKEVTWPEAESTVRSVLSPFSERLASVV
                     DAALTQRWVDVEPREGKV"
BASE COUNT          315 a          569 c          578 g          281 t
ORIGIN      
        1 gtcgggagac ggagcccagc ttgcgccagg caccggtccg gacggctcgc aggcggtgac
       61 gttgcagtcc gctggcagcg cgaatcagcc cggcgcggcg tcgtacatcc ggcgggatct
      121 tccggagggc gagtcgacgc tacacgtcgc gttcgatctt gatgcgcgag agttggatcc
      181 gggcgcgacc cggctagcca cgttcacatc ggccgactcg agcgcgatcg ctgcactcta
      241 cctgctgcca gatggaacgc tgggcgtgcg attcggcgaa tcggactcgt tggcgccggt
      301 cggatcgctg gacctctcga tttggaaccg aatcgagatc ggtatctccg caggaccgac
      361 tggtggtgac atgaccgtct gggtcaatgg gcaattctca ggcagcgcga ccacagcggc
      421 ggtaactgcc gagccgcgct cgatcctgat tggcggttgg gcgaccgatc gcacctacga
      481 tctgctggtc gacaacgtgg cgatcgaccg tggctgcctc tccgggtgtc cggaggctcc
      541 agcccccact gcgaccacca cgccggaacc ggcgcaggac gttccggtcg cgcccgagcc
      601 tggtacgccc gtcagtggat aatggcagcc ggtgaatcgg agcctgacac ggagacgtgt
      661 cgcacgctga cacgagggtg tatcgaacat gaccgaatcc gcgcttcccc gctgggacct
      721 gacggggatc tttccgaacc cgatcagcgg ggaatatctc gaagccgtcg ccaagctgcg
      781 cgccggaatc gagcagatga ccggactctt tgacgagttc ggcgttggtc tcgactccgg
      841 cgcgcatccg caaaccgaac cgggccggat catctccacc tggaacgaga tcggcgatct
      901 ggccgatttg ctcgacaact acctctactg ctggacatcg accgactcca acgacgccgc
      961 ggccgcctcg gcctacggcg agctcgacat gatcacctcg ccgatgcgcg ccttgcgctc
     1021 gcgactgaca acctggcttg gcacgctcaa cccggattcc tctaacgacc cagtcctcgc
     1081 gaatcatcgc taccaggttc agcaggcagg tatcggcgcg aagcacgtca tggcgccgga
     1141 gctggaggag ctggccggag cgctgcaggc aaccggaggg caggcctggg tacgatcgcg
     1201 cgatgaatcg gatgcggggc tgaccaccac catcacgatc gacggcgaaa cgcggacgac
     1261 gtcgctgact gagctggaca cgctgctctc ctcgccggat cgcgaaaagc ggcgggcggc
     1321 ggcggaggcg atggacgtgg cgcgcgagaa ggccgctccc cagttcgccg cgtcgctgaa
     1381 cgcgatcaag ggcgaagcat tgaccctttc gcggcgccgc aagtggggat cgccgctcga
     1441 ttggtcgctc ttcatcagcg cgatcgaccg ggagatcctc gacgcgatgt tcgccgccgc
     1501 ggagtcggtc catggtgagc tgcaccgcta cctacagacc aaggcccgac tgctcggcat
     1561 cgagaagatg gcgcactacg atctgtcggc gcccatcagc aaagaccaga aggaagtgac
     1621 ctggccagaa gcagagagca ccgtccggtc cgtcctcagc cccttctccg agcggctggc
     1681 gtcggtggtc gatgccgccc tgacccagcg gtgggtcgat gttgagccgc gcgagggcaa
     1741 ggt
//
LOCUS       DATAOZ010000121         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5311693, whole genome shotgun sequence.
ACCESSION   DATAOZ010000121 DATAOZ010000000
VERSION     DATAOZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5311693"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VHR64_03405"
     CDS             <1..681
                     /locus_tag="VHR64_03405"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX3301901.1"
                     /translation="DPSVVAGHSLGEFTALVAAGVMDFPSGLQLVRERGRLMKEAGEE
                     NPGGMAAVIGLDADTLATVCHDASHGDVITVANDNCPGQTVISGEVAALLRAMEMAKA
                     RGAKRVARLGISIASHSPLMARASGSLRDIMSRLPLGAPQIPVVANVTATAMLTVDDV
                     RQELAHHVERPVNWTRSVAEMVNSGATTFIEVGPGQVLSGLIRRINRDVTTLSLTDLG
                     LPGDVPNN"
     gene            805..2043
                     /gene="fabF"
                     /locus_tag="VHR64_03410"
     CDS             805..2043
                     /gene="fabF"
                     /locus_tag="VHR64_03410"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX3301902.1"
                     /translation="MKRVVVTGLGAISPLGIGVPALWEGLLAGCSGVRRVQHFDTENL
                     VVKIAAEVPDFDPKAFMDPKSVRRMDRFAHFAIAGTREALADAALEITDENRDRVAVV
                     MNTGGGGIPTIESNVTAMALHGPSRVGPLVIPMFAPNMASSQVSIAYGIQGPTITSAA
                     ACASGVQAFVDAVRLLQAGDADVAVTGGTEAGITPVALTSLANMHALSRRNDDPQGAS
                     RPFDRERDGFVFGEGAGVLILETEEHAVRRGASVYAEALGGALTSDAFHLTAPEPSGR
                     GARMAMTNALKKAAMEPHELDYIAAHATATQIGDIAETLAIKDALGDHARRVAISANK
                     SMIGHMLGAAGAISAVACVLAIRDGVVPPTVNLDYPDPDCDLDYVPHVARKMPVRAAM
                     VNGFGFGGQNASTIFRRFDG"
     gene            complement(2064..2618)
                     /locus_tag="VHR64_03415"
     CDS             complement(2064..2618)
                     /locus_tag="VHR64_03415"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX3301903.1"
                     /translation="MGEPPIRPCPYPPPPPALRPWNPRAPEIAARVMALIAKRLPDTT
                     IEHVGSSSVPGCAGKGVLDLVIPYRGDTHLGVINEALFALGFGRQRNRDPFPETRPMR
                     IGAFDHEGERFLLHVHVVPVRTHDLAELVDFRDRLRADPALVADYVAAKRAILDAGVR
                     DGTDYAERKGEFIAALGYKGAEEA"
     gene            complement(2624..>3108)
                     /locus_tag="VHR64_03420"
     CDS             complement(2624..>3108)
                     /locus_tag="VHR64_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX3301904.1"
                     /translation="AGGAVPGPFDCWLVLRGIKTLPVRMERHSRNGLAVAEFLREHPA
                     VRQVFYPGLADHPGHDVAARQMRDFSGMVSFTVRGDFQTAAEVARSTRIFQLAVSLGG
                     VESLIEHPGQMTHVSVAGTEAEVEDTLLRLSVGIENEADLIGDLKQALEVVVPATASR
                     "
BASE COUNT          536 a         1013 c         1025 g          534 t
ORIGIN      
        1 gatccaagcg tggttgccgg tcactcgctc ggcgagttca ccgcgctcgt cgccgccggc
       61 gtgatggatt tcccatccgg gttgcaactg gtcagggagc gtggccgtct catgaaggag
      121 gcgggtgaag agaaccctgg cggtatggcc gcggtgatcg gtctggacgc cgacaccctg
      181 gcgacagtct gtcacgacgc gtcccacggc gacgtcatca ccgtcgccaa cgataactgt
      241 cctgggcaga cggtgatttc cggtgaggtt gccgcactcc tgcgggcgat ggagatggcg
      301 aaagcgcgtg gcgccaagcg cgtggcacgg ctcggtatca gtatcgcctc gcactcgccc
      361 ttgatggctc gcgcttcggg gagtctgcgc gacatcatga gccggttgcc gcttggcgct
      421 cctcagatcc cggtcgtggc caacgtgacc gcgaccgcaa tgctcaccgt cgacgacgtg
      481 cgccaggaat tggcgcatca tgtcgagcgg cccgtgaact ggacgcgctc ggtggccgag
      541 atggtcaaca gcggcgcgac cacttttatc gaggtgggac ccggtcaggt actctccggg
      601 ctcatccgcc gcattaaccg ggatgtcacc acgctcagtc tgaccgatct cggtttgcca
      661 ggcgacgtgc cgaacaactg atcctgcgct acgcctcacg ccctcgccca tctcgaaggg
      721 agagggggaa cggacggcgg atgatgacgt gccgatcgca aaccgagcgg aatctctatt
      781 gcacctgaca gagagggaga gatcgtgaaa cgggtcgtgg tgaccggtct gggggcgata
      841 tcgccgctcg gcatcggagt ccccgcgcta tgggaaggat tgttggccgg atgctccggc
      901 gtccgtcgcg tccagcattt cgataccgag aatctggtcg tcaagatcgc cgccgaagtt
      961 cccgacttcg accccaaagc gttcatggat ccgaaatcgg tgcgccgcat ggaccgcttc
     1021 gcgcatttcg cgattgccgg gacccgggag gccctcgcgg acgcagcgct ggagattacc
     1081 gatgagaacc gcgaccgtgt cgccgtggtg atgaatactg gcggcggcgg cattccgacg
     1141 atcgaaagca atgtcaccgc catggccctg catggcccat cccgcgtcgg gccgctggtg
     1201 attccgatgt tcgccccgaa tatggcgagt agtcaggtct cgattgccta tggcatccag
     1261 ggacctacca tcacctccgc ggctgcctgc gcctccggcg tgcaggcgtt cgtcgatgcg
     1321 gtgcgcctgc tgcaagccgg cgatgcggac gtcgccgtga ccgggggcac cgaggcgggg
     1381 atcaccccgg tcgcattgac cagcctggca aatatgcacg cgctctcacg acgaaacgac
     1441 gatccgcagg gcgcgtcacg gccgttcgat cgggagcgtg atggttttgt cttcggggag
     1501 ggcgcgggcg tgctgatcct cgagactgaa gagcatgcgg tccgccgcgg cgcgtcggtg
     1561 tatgccgagg cgctcggtgg tgcgctgacg tccgatgcgt tccacctcac cgcgccggaa
     1621 ccgtcaggac gcggcgctcg catggcgatg acgaatgctc tgaaaaaagc cgcgatggag
     1681 ccgcacgagc tggattacat cgccgcgcat gccaccgcga cgcaaatcgg cgacatcgcg
     1741 gagaccctgg ccatcaagga cgctctcggc gaccacgcgc ggcgggtcgc catctcagcg
     1801 aacaagtcga tgatcggtca catgttaggc gcggccggcg ctatctcggc cgtggcctgc
     1861 gtcctggcga ttcgcgacgg ggtcgttccg ccaacggtca atctcgatta tcccgacccc
     1921 gactgcgacc tcgactacgt accgcacgtg gctcgcaaga tgccagtgcg cgcggcgatg
     1981 gtcaacggct tcggcttcgg cggccagaac gcctcgacca tcttccgccg tttcgacggg
     2041 taaccagcga cacggttgac tcgtcaggct tcttccgccc ctttgtagcc tagagcggca
     2101 atgaactcgc ctttgcgctc ggcatagtcg gtgccatccc gcaccccggc gtccaggatg
     2161 gcgcgtttgg cggcaacata gtccgctacc agcgccggat ctgcacgcag ccggtcccga
     2221 aaatcgacca actcggccaa atcgtgcgtt ctgacgggca cgacatggac gtgcagcaga
     2281 aacctctccc cctcgtgatc aaaggctccg atccgcatcg gccgcgtctc ggggaacggg
     2341 tcgcgattcc gctgtcgccc gaacccgagc gcgaagagtg cctcattgat gacgccgaga
     2401 tgcgtgtcgc cgcgataggg aatgacgaga tcgagaacgc ccttgcccgc acaacccggc
     2461 acggagctac tgccaacgtg ctcgatcgtc gtatctggta atcgcttggc gattaatgcc
     2521 atgacgcgcg ccgcgatctc cggcgcccgt gggttccacg gccggagagc gggcggtggc
     2581 ggcgggtatg ggcaggggcg gatcggcggt tcgcccacgg aaattaccga ctcgccgtcg
     2641 ccggtacgac cacctcgagg gcctgcttca gatcgccgat gagatccgcc tcgttctcga
     2701 tgccgaccga gaggcggagc agggtatcct cgacctcggc ctcggtgccg gcgacgctga
     2761 cgtgcgtcat ctgaccgggg tgctcgatca gcgactccac acctccgaga ctgacggcga
     2821 gttggaagat gcgcgtgctg cgcgccacct ccgccgccgt ctggaaatcg ccgcgcacgg
     2881 taaaggagac catgccggag aaatcgcgca tctgccgggc cgcgacgtcg tgaccgggat
     2941 ggtcggcgag tcctgggtag aagacctgac gcacggccgg atgttcgcgc aggaactcgg
     3001 cgacggccag cccattgcgg ctgtggcgct ccatccgcac cggcaacgtc ttgatgccgc
     3061 gcagtacgag ccagcagtcg aacggccccg gcaccgcgcc gcccgcgt
//
LOCUS       DATAOZ010000122         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5575169, whole genome shotgun sequence.
ACCESSION   DATAOZ010000122 DATAOZ010000000
VERSION     DATAOZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5575169"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VHR64_03425"
     CDS             complement(<1..953)
                     /locus_tag="VHR64_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3301905.1"
                     /translation="MTTQRPVEQAIEALMSEQRTFAPAPEFVAQSAIKDRSLYERADA
                     DIEAFWAQEAESLSWYRKWDTVLEWDPPFARWFSGGQLNVSYNCLDRHVEAGRGDKIA
                     YYWEGEPGDRREITYKQLLDEVCQCANALKELGVARGDRVAIYMPMIPELPIAMLACA
                     RIGAPHTVVFGGFSATALSDRIIDAEARLVITADGGYRRGSAAGLKGNVDEALQSTPS
                     IEHALVVKRTGQDTNMTEGRDIWWHDIVPKQDATCEPERMESEDMLYLLYTSGTTAKP
                     KGILHTTAGYLLGSAATHRWIFDLHDDDIYWAAADIGWVTG"
     gene            1157..1516
                     /locus_tag="VHR64_03430"
     CDS             1157..1516
                     /locus_tag="VHR64_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301906.1"
                     /translation="MVTVHMIVGSLVVIAFIVLTVSYGMQLNGTRTLTWSRPLSMGAA
                     GLLLLQYVLGFGLLADHDITAAHYLFALAAIIPVGYEHMVASTQEVPSTRARLGMFAA
                     AAATLLVLVAYAIAESR"
     gene            1520..>1882
                     /locus_tag="VHR64_03435"
     CDS             1520..>1882
                     /locus_tag="VHR64_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3301907.1"
                     /translation="MAHDIDICVVGGSIAGSAAAATFAREGYSVALIEREAAFRDKAR
                     GEGIHPWGMDEAEDLDLLDVVRAGGAHPLPTWLTYVDRVPLEPMVMAEHSARGQVEQG
                     SFHPRLQETMLDYAREQGV"
BASE COUNT          301 a          609 c          612 g          360 t
ORIGIN      
        1 tgcccggtga cccagccgat atccgcggcc gcccagtaga tgtcgtcgtc gtgcagatcg
       61 aagatccagc ggtgggtggc ggccgagccc agcaggtatc cggcggtcgt gtgcaggatg
      121 cccttgggct tggcggtcgt gccggaggtg tagaggaggt agagcatatc ctcgctctcc
      181 atcctttccg gctcgcaggt cgcgtcctgc ttcgggacga tgtcgtgcca ccagatatcg
      241 cggccctcgg tcatgttcgt gtcctgaccg gtccgcttga caaccaatgc atgctcgatc
      301 gacggcgtcg attgcagcgc ctcgtcgacg ttgcccttga ggccggctgc cgatccgcgc
      361 cggtatccgc cgtccgcagt gatgaccagc ctcgcctcgg catcaatgat ccggtcggag
      421 agtgccgtcg ccgagaagcc gccgaagacg accgtgtgcg gcgcgccgat ccgggcgcag
      481 gcgagcatgg cgatcggaag ctcggggatc atcggcatgt agatggcgac ccggtcacct
      541 cgcgcgaccc cgagctcctt gagggcattc gcacactggc agacctcgtc gaggagctgc
      601 ttgtaggtga tctcgcgccg gtctcccggc tcgccttccc agtaataggc aatcttgtca
      661 ccgcgccccg cttcgacatg gcggtcgagg cagttatagg agacattgag ctgcccgccg
      721 ctgaaccacc tggcaaatgg cggatcccac tccaggacgg tatcccattt ccgataccag
      781 ctcagcgatt ccgcctcctg ggcccaaaac gcctcgatgt cggcatcggc gcgctcatac
      841 agcgaccggt ccttgatagc cgactgggcg acgaattcgg gcgcgggcgc gaaggtcctc
      901 tgctccgaca tcaatgcttc gatcgcctgc tcgacgggac gttgggttgt catcagggct
      961 cctttatcag ggtgggcacg tacggcgtga ccggttctat tccgggcaga gcaacccggt
     1021 tgtcgctacc acgacgggtc aattggcagt atacaaacgg aagcggccgg ccgggctgcg
     1081 ccgatttcgc tgaaatcagg ttccagggcg cggcgacccg gccagatgag accggcaaga
     1141 ccggaggagg ggttccgtgg ttacggtgca catgatcgtc ggctcgttgg tggtgattgc
     1201 cttcatcgtt ctgaccgtct cgtacggcat gcagctcaac ggcacgcgca cgttgacctg
     1261 gtcgcgtcca ctttcgatgg gcgccgccgg tctcctgctt ctccagtacg ttctcggctt
     1321 cggattgctg gccgatcacg acatcaccgc cgcccactac ctcttcgcac tggccgcgat
     1381 catcccggtg ggctatgagc acatggtcgc gagcacgcag gaagttccat ctactcgcgc
     1441 tcgcctcggc atgttcgcgg cggcggcggc gaccctgctt gtcctggtgg cctacgcgat
     1501 cgccgaatct cgctagctca tggcgcacga tatcgacatc tgcgtcgtcg gcggcagcat
     1561 cgccggctcg gcggctgccg ccaccttcgc gcgggagggg tattccgtcg cgctgatcga
     1621 gcgggaggct gccttccggg acaaggcgcg cggcgaaggc atccatccct ggggtatgga
     1681 tgaggcggaa gacctggatc tgctcgatgt cgtccgggcc ggcggcgcgc atccattacc
     1741 gacctggctg acctacgtcg accgtgtccc gctcgagccc atggtgatgg cagagcactc
     1801 ggcccgcgga caggtcgagc agggcagctt tcatcccagg ctccaggaaa cgatgctgga
     1861 ttacgcccgg gaacaggggg tc
//
LOCUS       DATAOZ010000123        19545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5575231, whole genome shotgun sequence.
ACCESSION   DATAOZ010000123 DATAOZ010000000
VERSION     DATAOZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 19545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19545
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5575231"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VHR64_03440"
     CDS             complement(<1..284)
                     /locus_tag="VHR64_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301908.1"
                     /translation="MNLAWYARRLTRMSPAEVRGRLADAWVHRRWRARQVRPGEPDPL
                     PLPAARPDFASPLDPALTTALPAEAGARLLRTADAALTGRFRVFDRERDDL"
     gene            complement(281..2521)
                     /locus_tag="VHR64_03445"
     CDS             complement(281..2521)
                     /locus_tag="VHR64_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HEX3301909.1"
                     /translation="MKQIAQNYKSGEIALLEVPLPACRSGGVLVRTEFSLISMGTEAM
                     KIHESKLSLIGKARARPDQVKKVMQTMAQLGPVATYQKVMNRLDSYTALGYSLSGVVV
                     AVGAGAEEFKVGQRVACAGNQFALHAEYNWVPRNMCVAVPDGVAPVQAAFTTVAAIAM
                     QGFRQADARLGETACVIGLGLVGQILVQILRAAGLQVVGVDVANDRCELAIAGGAAAC
                     GMRGDSSFEAMKTRLLALTSGAGADCIFLTAGGSTNQPVEVAAELARDRARVIDIGKC
                     KLDLPWNAYYGKELDVRFSRSYGPGRYDPNYEERGIDYPIGYVRWTERRNMECVLDLL
                     AAGKLNFDAITSDVVAFADAVEVYEKVAKGELRGLGIVFEYPSGGSAVEALASHPAAA
                     PVKLHRRRPSTAGRMRLGVIGCGNYATTMLLPHLKRHPEVELVEVATATSLSAANARA
                     KFGFGRVSTDFPSLLADPEIDAVLIATRHDSHADIVCRALRAGKAVFVEKPLALTAEQ
                     LAAVRDAVAETGNDRVMVGFNRRFAPALTALREAWGGSGGPQVVSYSVNAGPLDASSW
                     YAQPESQGTRFAGEGGHFIDTISWWLGADPLAAQAVATPGEPDNLLALLTYPDGSVAK
                     IAYLTKGDPRYPKEVLEVFGDGKVARMDNFRRTELWRGGRRKRTRVGLDKGQKHALDA
                     FVGAVRTGADMPVALSSLIATTAGTLAVGRSIASGRCEPVAGWECPPEQGVADDRSCY
                     ELRAAQ"
     gene            complement(2568..4484)
                     /gene="asnB"
                     /locus_tag="VHR64_03450"
     CDS             complement(2568..4484)
                     /gene="asnB"
                     /locus_tag="VHR64_03450"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX3301910.1"
                     /translation="MCGVVGLYNFPATQDVLARMLGAIAHRGPDAEGILECRSHGADV
                     RLGHRRLSIIDLSDAANQPFEKEGLVIVYNGEIYNYRALRQELAAAGVRFRTTSDTEV
                     LLEAWRRWGADSLNRLRGMFAFAMLDQRTGRLILARDPFGIKPLFIARRPGGLAFASE
                     LKALTLALGDTLELDATALISSLMYYWVPEAHCVYRGVEKLPPGHWAEVAPDGSYRQA
                     CYFDPQREFVHDAYQEIDVPDLRAIIEGSVAVHMVADVPVSAFLSGGLDSSLIAAIAA
                     RQGHGLEGYTISFRPEDRKLEAMPDDLFYARKIASKYRIKLHEIEIAPDVAEILPRMV
                     TILDEPIGDAAAINAYLICKAARAAGVKVLLSGMGADELFGGYRKHLACMLASRYRSL
                     PSFIRDRLVRPTVAGLPVAGKNKGYRTVRWAKRFLAFADLPEEAAFRRSYTHCGLPEF
                     QGLLNPELLGVVDRLIEEHAELYNQYRMGNCRDQVNSMCYTDVRHFLPGLNLAYTDRA
                     SMAASTEVRVPYVDREVAAAAFAISGSRKIAGWNTKAVLKKAAEPYLPRSVIYRPKGL
                     FSAPLRAWIRRDLQVMVEDLLMGGRMTRSGFLNAGYLRRMVQEDRAGIADRSKEIWQL
                     MTLETWLQQQPASL"
     gene            complement(4510..5166)
                     /locus_tag="VHR64_03455"
     CDS             complement(4510..5166)
                     /locus_tag="VHR64_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX3301911.1"
                     /translation="MIALQVAQPGRTLSVLCLGAHSDDIEIGAGGTLLGWIASGVRLE
                     VCWCVLSATGPRAAEANASAAAFLSGAVRARVELAQFPDGLFPYHGHEIKAWLEDLRA
                     GIEPDVILTHRGGDAHQDHREVSGLTWNLFRDHLIFEYEIPKWDGDLGRPNFYVPLTT
                     TILERKIELLHAHFGSQRSKQWFDAETFRGLARLRGMECRAPERYAEGFAVRKARLGE
                     "
     gene            complement(5163..5975)
                     /locus_tag="VHR64_03460"
     CDS             complement(5163..5975)
                     /locus_tag="VHR64_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX3301912.1"
                     /translation="MKVVLFCGGLGTRIREYSENIPKPMIPVGHQPILWHVMQYYTEY
                     GHTDFILTLGYKANIIKEFFLNYRPQAFADCVVSGFGANVELLQEPEVDWRVTLIDTG
                     IWRSIGERLWTVRDYVKNEEIFLANYSDGLTDVNLDDMITRFRNSDKLACFLAVRPPL
                     TYHLADIEADGRVREFRSSDRSDIWINAGYFILRPEIFDYMNEGEELVLEPFWRLIEA
                     NKLMAYKHEGFWRCMDNLKDRQVLEEMIEQGRMPWRAGDTTKRGGTLSIASL"
     gene            complement(5972..7264)
                     /locus_tag="VHR64_03465"
     CDS             complement(5972..7264)
                     /locus_tag="VHR64_03465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3301913.1"
                     /translation="MSIQVLEPSPLTSVPTARSCRLCGAALEHNFVDLGMSPLCESFL
                     AAGQLDAVESYYPLRVWVCGSCFLVQLGEYVSPENIFREYAYFSSYSTTWVAHAKAYC
                     EMIAPRLGLGPDSLVVELASNDGYLLQHMLPLKIPVLGIEPAANVAKVALDKGIATRI
                     DFFGVELAQRLVDEGRRADLIVGNNVLAQVPDLNDFVAGMARLLASNGVITLEFPHLE
                     RLMAENQFDTIYHEHFSYFSLIAIERLAARHGLRLIDVDELTTHGGSLRVYLAHLGSA
                     WPTADRVQDLLARERRLGFGEIAIYASFAGRVTRTKRRLLSFLIAAKDAGKVICGYGA
                     PGKGNTLLNYCGIGTDFLEFTVDRNPYKHGRFTPGMHVPIRPVEAIDDLAPDYILILP
                     WNLKDEIIQQMRHVGAWGGKFIVPIPDLTVIDPKEPRS"
     gene            complement(7659..8591)
                     /locus_tag="VHR64_03470"
     CDS             complement(7659..8591)
                     /locus_tag="VHR64_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301914.1"
                     /translation="MPSVFFDPDISDDERRRRIYAGEIVILSPTPGTTALAALAKAML
                     EEAFAPHDPRLVHEHMTPDQVAAILAELKPKFIHHPQCKQFIPTIMSENGVHVDKLYF
                     DVPRLRSAYPSHFLSSGIAYAFHPHRDTWYSAPMCQINWWIPIYPLDPNNAMGFYPRY
                     ATEPVKNNSEIYNYYEWNTKNRASAAQHVRGDSREQPKPQQELEPITIRYLPPPGGVI
                     VFSGAQLHETVPNTTGLARYSIDFRTVHLEDLAAYHGAPNLDSRSTGTTIRDYLRASD
                     LQHLPEEIIALYDDGSASPDRVLYFGDRLLNKTA"
     gene            9060..10568
                     /locus_tag="VHR64_03475"
     CDS             9060..10568
                     /locus_tag="VHR64_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX3301915.1"
                     /translation="MVRRALAMAAGEQYLRLLINFVLIATISRLLTPSEFGVSVIGTG
                     IMAIALGLREFATSDFLIQRQEVAREDVRTSFTLAFLLTVLITVAMFALAPWFGTLYG
                     ENGLASFVRIAAVSGLVEAVSLPIRGLLRRDLEFGTLAIVNTASAITTAAVTIVLALA
                     GFSYMSVAWAKLASAATTTALSFYFRPDLSILRPAVVSWGSVLAFGGYSGASFVINRA
                     YESLPQLVLGQLLPHSAVGLYNRAQTVSDIPDRIVLTSVFSVAFPAFAAEIRSGRSLK
                     EPYLRALGYITVFYWPALILLTILADPVVSLFLGAQWRSVTPLLQVMAIAGLAWFPVL
                     LTSPVLLAVSANRDRVLADLLGRSVSAAILCASASFGIMAMAVSKLVTLPFQMIISFC
                     FVRRHICFRWSEVAVALWRSAVVMLGSAAGPACVVAFSDGGFDLSIGASLLAMLLAVI
                     GWLTAVLAVQHPVLLELGRARDHLSEVLRPKLFGLPRAGHGARPEGQGARTV"
     gene            complement(10696..11235)
                     /gene="rfbC"
                     /locus_tag="VHR64_03480"
     CDS             complement(10696..11235)
                     /gene="rfbC"
                     /locus_tag="VHR64_03480"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX3301916.1"
                     /translation="MGFEETNLSGAWLIHLIPARDHRGFFARTFCVQAYADQGLTTGF
                     VQHSTSQSVTRGTLRGMHFQRAPHAEVKVVRCLKGAIWDVIIDLRPDSPTYRHWQGFE
                     LTADNQLQLYVPEGFAHGFQTLCDDTQVGYLISAFYAPQAASGVRYNDPAFAIDWPLP
                     VTEISEKDRSWPDFRVPVS"
     gene            complement(11506..12870)
                     /locus_tag="VHR64_03485"
     CDS             complement(11506..12870)
                     /locus_tag="VHR64_03485"
                     /inference="COORDINATES: protein motif:HMM:NF027579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HEX3301917.1"
                     /translation="MAAVESVKHLLDDPSIGDQIFALAAEIFPICRSITGDGIRQTLE
                     RLARHVELEQHEVPTGTQVFDWSVPPEWTIRDAYIKDPSGCRIVDFQASNLHVLNYSR
                     PVHARISLDELKEHVFTLPDQPDLIPYRTSYYAERWGFCMAHTHLAALPEGVYEVMID
                     ASLDAGSLTYGEYLHRGATDDEVLFSAHICHPSLANDNCSGLALLTRLAARLASLETR
                     YSYRFVFAPGTIGAIAWLARNEHRLARIRHGLVLSCVGDAGGPTYKKSRRGDAFIDRA
                     MAHVLQHAAPSPTILDFFPYGYDERQYCSPGFNLPVGLFQRSQFGTFPEYHTSADNLD
                     FIGPEHLASSYRMIVGLIEVLERDWWPRSTTPKCEAQLGRRGLYGALGGDPEAAAKSM
                     AFLWILNLADGRHCLLDIAERAKLPFGVIADAAHLLKTSGLLVEDGGEAPPSSGAKPS
                     PPTE"
     gene            complement(12902..13972)
                     /locus_tag="VHR64_03490"
     CDS             complement(12902..13972)
                     /locus_tag="VHR64_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3301918.1"
                     /translation="MRVLVTGHRGYIGTVMVPMLLDAGHEVTGYDSDLYERCTFAIGG
                     SIAEVPSLRKDVRDVTVDDLVGFDAVIHLAALSNDPLGDLNPEITYDINHRASVRLAT
                     LAKRAGVARFLFASSCSNYGLAGDGMIDETGALNPVTAYGRSKVQSERDIARLAGDGF
                     CPTYLRPATAYGLSPRLRVDIVLNDLVASAVTKGLIYLKSDGSPWRPIVHIEDIARAF
                     LAGLEAPADKVFNEAFNVGRTEHNYRIREIAEVVAQVVPGCRLEIAPDAGPDKRSYRV
                     SFDKIARVLPEFEPRWDVRKGAEQLHAAYRAAGLTLEQIERRYQRISQIKMLMREGRV
                     GDDLRRLDLPAPEFALAAAATA"
     gene            complement(14211..15617)
                     /locus_tag="VHR64_03495"
     CDS             complement(14211..15617)
                     /locus_tag="VHR64_03495"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF004856.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX3301919.1"
                     /translation="MQLNGHQVHQLGLRSAQASPSLQARAHQLIPGGCHTYAKGDDQY
                     PILAPAFIARGSGCRIWDMDGREYIEYGMGNRAVGLGHAYPPVVEAVQRELLRGSNFT
                     RPSPIEPACAEAFLELVDGAEMVKFCSDGSDATSGAVRLARAYTGRDLIACCADHPFF
                     STDDWFIGTTPMNAGVPEAIRQLTLTFHYNDIAGVEALFEQHPGRIAALILEPARTED
                     PPLGYLQALQRLCHANGALLILDEMITGFRWHNGGAQKLYGIVPDLATFGKALANGFS
                     VSALAGRREFMRLGGLDHRDRPRVFLLSTTHGAETHALAAAIATMQVYRRENVIEHLY
                     RQGTRLREQAEQAIRRHGLAEFVQILGKPCCLSYATLGPDGRPSQAFRTLFLQETIRR
                     GVLMPSLVVSYSHTDRDIDLTVAAFNGALEVYRLALDHGVERYLVGRPSQTVYRRYNH
                     PDGPPQRPSAVASEIAAR"
     gene            complement(15951..16838)
                     /locus_tag="VHR64_03500"
     CDS             complement(15951..16838)
                     /locus_tag="VHR64_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEX3301920.1"
                     /translation="MNTNEATSRGLGWSEIGLRVGGTIAETSLDEFGLKSIDLDARRA
                     RSDAAPPGAGEAAASGATLIYLDTRTFTRDCIGRWLESHLSGFSIRALPDPEQILTAP
                     DLGNEIQVVIIATGPERMSSRGVASLMARVAELMPSVPVAVLSDYEDAENIRAAFELG
                     VRGYIPTSLASLVAVEAVHLICAGGTFAPATALLSSSAGAGDESDSPPLIEGFTQRQA
                     QILDCLRRGMANKLIAYELTMCESTVKVHIRNIMKKLKATNRTQVVYLTQGLFEEGGH
                     HRRPADTIRRSTSGPDLRV"
     gene            17607..18236
                     /locus_tag="VHR64_03505"
     CDS             17607..18236
                     /locus_tag="VHR64_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3301921.1"
                     /translation="MRRQLAMLQVERNAALEVLDRLPTAVVLCDQSGTPIIINGAARE
                     ILASKDGLVVRGGRLAARRRLETAALENLIVGAASAIGESGSSGGTMSVSRASGSRPL
                     SVLVSPMRAPGAVPGFGQIAAALFISDPESVILTNEDRLLHLYGLTPAESRLAAKLAQ
                     GRSLEEAAAMLNIKIQTARSYIKRILNKTGTKRQVELVRLLLLSPAHLS"
     gene            18490..19188
                     /gene="sfsA"
                     /locus_tag="VHR64_03510"
     CDS             18490..19188
                     /gene="sfsA"
                     /locus_tag="VHR64_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEX3301922.1"
                     /translation="MRWPEPLIPGRLVRRYKRFLADVALEDGRQVTVHCPNPGRMLGL
                     DAPGAQVWLSRSASASRKLPLTLELVEVADGLVGINSMHPNRLVEEALRGGLIAELAG
                     YPSIRREIAYDRGCRVDFLLSGGGRPDCFVEVKNVHLKRTLGAEFPDTATARGVKHLK
                     ALSRAAAAGARAVVLYVVQRIDCADFRLASDLDPGYAAAARTAGTAGVESLCRACAIT
                     LDEIRLAAALPIVP"
     gene            19207..>19545
                     /locus_tag="VHR64_03515"
     CDS             19207..>19545
                     /locus_tag="VHR64_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX3301923.1"
                     /translation="MMQDQLHTAKRSASEVQVPLHDATGFVGMGRAGQLAAAVLDMID
                     PHVRPRVSTGELDRLCHAFILDHGAAPAPLNYRGFPKSICTSINHEVCHGIPSEGRVL
                     RDGDIVNIDVT"
BASE COUNT         3381 a         6315 c         6462 g         3387 t
ORIGIN      
        1 aggtcgtcgc gttcccggtc gaagacgcgg aaccggcccg taagcgcggc atcggcggtc
       61 cgcagcagcc gcgccccggc ctcggccggc agggcggtgg tcagggcggg gtcgagaggg
      121 ctcgcaaaat ccgggcgcgc agccggcagc ggtaacgggt ccggctcacc gggccggacc
      181 tggcgcgcgc gccagcggcg gtgcacccag gcgtcggcca ggcgcccccg gacttccgcc
      241 ggcgacatcc gcgtgagacg ccgggcatac caggccagat tcactgcgca gctcgcagct
      301 cgtagcagct ccggtcgtcg gccacccctt gctctggcgg gcattcccag ccggccactg
      361 gctcgcaacg cccgctcgcg atgctgcgcc cgaccgcaag cgtgccggcg gtggtcgcga
      421 tcagggaaga cagcgcgacc ggcatgtccg cgccggttct caccgccccg acgaaagcgt
      481 cgagggcatg cttttgcccc ttgtccaggc cgacgcgcgt ccgcttgcgt cggccgccgc
      541 gccagagctc ggtgcgccgg aaattgtcca tccgcgcaac cttgccgtcg ccgaagacct
      601 ccaggacctc cttcgggtat ctcggatcgc ctttggtcag ataggcgatc ttcgcgaccg
      661 agccgtcggg ataggtcagc agcgccagca ggttgtcggg ctctccgggc gtcgcgacgg
      721 cctgcgcggc cagcggatcg gcgccgagcc accaggagat ggtatcgatg aaatgtccgc
      781 cttcgccggc gaagcgggtc ccctgagatt cgggctgcgc ataccagctg ctggcgtcca
      841 gcgggccggc attgacggaa tagctcacga cctgcggccc accggagccg ccccaggcct
      901 cgcgcagcgc cgtcagcgcc ggagcgaagc ggcggttgaa gccgaccatg actcggtcgt
      961 tgccggtttc cgcgactgcg tctcgcaccg cggccagctg ctcagccgtg agcgcgagtg
     1021 gcttttcgac gaagacggcc ttgcccgccc gcagcgcgcg gcagacgatg tcggcgtggc
     1081 tgtcgtgccg ggtggcgatc aggaccgcgt cgatctccgg gtcggcaagg aggctcggga
     1141 agtcggtcga gacacggcca aagccgaact tggcgcgggc attggcggcc gacagtgagg
     1201 tggccgtcgc cacctcgacg agctcgacct ccgggtgccg cttgaggtgc ggcagcagca
     1261 tggtcgtcgc gtagttgccg caaccaatga cgccgagccg catccggccg gcagtcgacg
     1321 ggcgccggcg atgcagcttg accggtgctg ccgcaggatg gcttgcgagg gcctcgacgg
     1381 cgctgccgcc cgagggatac tcgaagacga tgccgagccc gcgcagctcg cccttggcga
     1441 ccttctcgta aacctcgacc gcgtcggcga acgcgaccac atcgctcgtg atcgcatcga
     1501 agttcagctt cccggcggct agcaggtcga gtacgcattc catgttccgg cgctcggtcc
     1561 accggacgta gccgatcggg taatcgatgc cgcgctcctc gtagttcgga tcgtagcgac
     1621 cggggccata ggagcgggag aaccggacgt ccagctcctt gccgtagtag gcgttccagg
     1681 gcaggtcgag cttgcacttg ccgatgtcga tcacgcgggc ccgatcccgc gccagctcag
     1741 ccgcgacctc cacgggctgg ttggtgctgc cgccggcggt caggaagatg cagtccgcgc
     1801 cggccccgct ggtcagggcc aggagcctcg tcttcatggc ttcgaaggac gagtcgcccc
     1861 gcatcccgca ggcggcagcg ccgccggcaa tcgcgagctc gcagcggtcg ttggcgacgt
     1921 cgacgcccac gacctgcagg ccggccgcgc gcaggatctg aaccagaatc tgaccaacca
     1981 gcccgagccc gatcacgcag gcggtctcgc cgagcctggc atcggcctgg cgaaagccct
     2041 gcatcgcgat cgcggcgaca gtcgtgaagg cggcctggac cggtgcgacg ccgtccggta
     2101 ccgcgacgca catgtttcgc gggacccaat tgtactcggc gtgcagagcg aactggttgc
     2161 cggcacaggc gacccgctgg ccgaccttga actcctcggc gccggcaccg acggcgacca
     2221 ccacgcccga gagcgagtag ccgagggccg tgtagctgtc cagccggttc atgaccttct
     2281 ggtaggtggc gaccggcccg agctgggcca tggtctgcat caccttcttg acctggtccg
     2341 gtcgcgcccg cgccttgccg atcagcgaga gcttgctctc gtggatcttc atcgcctcgg
     2401 tgcccatcga gatcagcgag aactcggtgc gcaccagcac gccgcccgac cggcaggcgg
     2461 gcagcggcac ctcgagcagc gcgatctccc ccgatttgta gttctgtgcg atctgcttca
     2521 tggcccgtcg aaaccagctc ccaatattag cgatccagcc gggctcgtca taacgaggcg
     2581 ggctgctgtt gcagccaggt ctccaaggtc atgagctgcc agatctcttt cgagcgatcg
     2641 gcgatgccgg cccggtcctc ctgaaccatc cggcgcagat agccggcgtt caggaacccg
     2701 gatctggtca tccgtccacc catcagcagg tcctcgacca tgacctgcag gtcgcggcgg
     2761 atccaggctc gcagcggtgc gctgaacagg cccttgggtc gatagatgac cgatctcggc
     2821 aggtacggct cggctgcctt cttcagcacc gccttggtgt tccatccggc gatcttgcgc
     2881 gacccggaga tcgcgaaagc cgccgccgcg acttcgcggt caacataggg cacccggact
     2941 tcggtcgagg ccgccatgct cgcgcgatcc gtgtaagcga gattgagtcc agggagaaaa
     3001 tgccggacat cggtgtagca catgctgttc acctgatcgc ggcagttgcc catccggtat
     3061 tgattgtaca attcggcgtg ctcctcgatc agacggtcga ccacgccaag caactctgga
     3121 ttcagcaggc cctggaactc cggcaggccg caatgggtgt agctgcggcg gaaggcggcc
     3181 tcctcgggca gatcagcgaa ggccaggaag cgtttggccc agcggaccgt ccgatagccc
     3241 ttgttcttgc cagccaccgg caggcccgcg accgtcggcc gcaccaggcg atcacggatg
     3301 aagctcggca agcttcggta tctgctggcg agcatgcagg cgaggtgctt gcggtagccg
     3361 ccgaacagct cgtcggcacc catgccggac agcagcacct tgacgccggc tgcgcgcgct
     3421 gccttgcaga tcagatacgc gttgatcgcc gcggcatcgc cgattggctc gtcgaggatg
     3481 gtcaccatgc gcggcagtat ttccgcgacg tcgggcgcga tctcgatttc gtggagcttg
     3541 atgcggtact tgctggcgat cttgcgcgca tagaacagat cgtccggcat ggcctcgagc
     3601 ttccgatcct ccggacggaa ggagatcgtg tagccctcga gcccgtgacc ttgtcgggcg
     3661 gcaatcgcgg cgatcaagct ggagtccagg ccaccgctga ggaaggctga taccggcacg
     3721 tccgcgacca tgtgcacagc cacggagccc tcaatgatcg cgcgcaggtc ggggacatcg
     3781 atttcctggt aggcatcgtg cacgaactcg cgctgcggat cgaagtagca tgcctgccga
     3841 tagctcccat cgggagccac ctcggcccag tgcccgggcg ggagcttctc gaccccgcgg
     3901 tagacgcagt gcgcttcggg tacccagtag tacatcagcg acgagatcag cgccgtcgcg
     3961 tcgagctcca gcgtatcgcc gagcgccaag gtcagagctt tgagttcgga agcgaatgca
     4021 agcccgccgg gccggcgcgc gatgaacagc ggcttgatgc cgaagggatc ccgggcgagg
     4081 atcagccgac cagtccgctg gtcgagcatg gcgaacgcga acatgccccg cagacgattg
     4141 aggctgtcgg caccccatcg ccgccaggct tcgagcagga cctcggtatc agaggttgtc
     4201 cggaaccgca cgccggcagc cgcgagctcc tgtcgcagcg cccggtagtt gtaaatctcg
     4261 ccgttataga cgatgaccag cccctctttt tcgaacggct ggttggcggc gtccgacagg
     4321 tcgatgatcg atagccgacg atgacccaat cgaacgtcgg cgccatggct gcggcattcg
     4381 aggatcccct cggcatccgg accgcgatgc gcgatggcgc ccagcatgcg tgcaaggacg
     4441 tcctgagtgg ccggaaagtt gtagaggccg actactccgc acattgcgcc cgatcctcgc
     4501 ccagagccgt cactctccga gcctggcctt gcgcacggcg aagccctcgg cgtagcgctc
     4561 gggcgcccga cattccatgc cgcgcaggcg cgccaggccg cggaaggtct cggcatcgaa
     4621 ccactgcttg gaccgctggc tcccgaagtg ggcgtgcagc agctcgatct tgcgctccag
     4681 gattgtggtt gtgagcggca cgtagaagtt cggccggcct agatcgccgt cccatttcgg
     4741 gatctcgtac tcgaaaatca ggtgatcgcg gaacaggttc caggtcagcc cggacacctc
     4801 gcgatgatcc tggtgggcat cgccgccccg gtgcgtcagg atcacgtccg gctcgatccc
     4861 ggcgcgtagg tcctccagcc aggccttgat ctcgtggccg tggtagggaa agagcccgtc
     4921 tgggaactgc gccagctcga ccctggcgcg gacagcgccg gacaggaatg cggccgccga
     4981 tgcgttcgcc tccgcggccc gcggcccggt ggcgctcagc acgcaccagc agacttccag
     5041 ccgtaccccc gaggcgatcc aaccgagcag cgtgccgccc gcaccgatct cgatgtcatc
     5101 ggagtgcgcc ccgaggcaca ggaccgagag cgtccggccg ggctgcgcga cctgcaacgc
     5161 gatcataggg aggcgatcga caaggttccg ccgcgctttg tggtgtcgcc ggcgcgccac
     5221 ggcatccggc cctgctcgat catttcctct agcacctgtc ggtccttgag gttgtccatg
     5281 cagcgccaga agccctcgtg cttgtaggcc atcagcttgt tggcctcgat caggcgccag
     5341 aacggctcga gcaccagctc ctcgccctca ttcatgtaat cgaagatttc cgggcgcaga
     5401 atgaagtagc cggcgttgat ccagatgtcc gaacgatcgg acgaccgaaa ctcgcgcacc
     5461 cggccatcgg cttcgatgtc cgccagatga taggtcaaag gcggccggac cgcgagaaag
     5521 caggcgagct tgtcgctatt gcggaagcgc gtgatcatat cgtcgaggtt cacgtcggtc
     5581 agaccgtcgc tgtagttcgc gagaaagatc tcctcgttct tgacgtagtc gcgcacggtc
     5641 cacaaccgct cgccgatgct gcgccagatg ccggtgtcga tgagggtgac gcgccagtcg
     5701 acctccggct cctgcaacag ctcgacattg gcgccgaaac cggagacgac gcagtcggca
     5761 aacgcctgcg gccgataatt cagaaagaac tccttgatga tattcgcttt gtagccaagc
     5821 gtcaggatga aatcggtgtg tccgtactcc gtgtagtact gcatgacatg ccaaaggatc
     5881 ggttgatggc cgaccggaat catgggcttc ggaatgttct cggaatattc tcgaatccga
     5941 gtgcccagac cgccacagaa gagcacgacc ttcatgatct cggttccttc ggatcgatga
     6001 ccgtcaaatc gggaatcggg acgatgaact tgccgcccca agcgccgacg tgacgcatct
     6061 gctggatgat ctcgtccttg aggttccacg gcaggatcag aatgtagtcg ggtgcgagat
     6121 cgtcgatcgc ctcgaccggc cggatcggca cgtgcatgcc gggagtgaac cggccgtgct
     6181 tgtacggatt gcgatcgacc gtgaactcga ggaagtcggt gccgataccg cagtagttca
     6241 aaagcgtgtt gcccttgccc ggagcgccgt atccgcagat caccttgccc gcgtccttgg
     6301 ccgcaatcag gaatgacagg agacggcgct tggtgcgcgt gacccgcccg gcaaaggagg
     6361 cgtagatcgc gatctcgccg aagcccaggc gccgctcgcg ggcgagcaga tcctggacgc
     6421 ggtccgccgt cggccatgcc gagccgagat gggcgaggta aacccgaagc gagccgccgt
     6481 gggtcgtaag ctcgtcgaca tcgatcagcc gcaggccatg gcgggccgcc aggcgctcga
     6541 tcgcgatcag cgagaaataa gagaagtgct catgatagat cgtgtcgaac tggttctccg
     6601 ccatcagtcg ctcgaggtgc gggaactcga gcgtgatgac tccgttcgac gccaagagcc
     6661 gcgccattcc ggcaacgaag tcgttgagat cgggaacctg agcgagcacg ttgttgccga
     6721 cgatcagatc cgcccgccgg ccctcgtcga ccagtctctg cgccagctcg acaccaaaga
     6781 aatcaatgcg ggtcgcgatc cccttgtcca aggcgacctt ggcgacattc gcggccggct
     6841 cgattccgag cacggggatt ttgagcggca gcatgtgctg gagcaggtag ccgtcgttgc
     6901 tggcgagctc gacaaccaaa ctgtccggtc cgagcccgag ccgaggcgcg atcatctcgc
     6961 aataggcttt ggcgtgggcg acccaggtcg tcgaatagga ggagaagtag gcatattccc
     7021 ggaagatgtt ctcggggctc acgtattcgc cgagctgaac caggaagcag gagccgcaga
     7081 cccatacccg aagtggatag taggactcca ccgcgtcgag ctgtccggcc gccagaaagc
     7141 tctcgcaaag cggcgacatc ccgagatcga cgaagttgtg ctcgagcgcg gcgccgcaga
     7201 gccggcagga cctggcggtg ggcacgctgg tcaggggcga cggctcgaga acttgaatgc
     7261 tcataccata ttcctgccga tccagtgtcc cgccgatgac atgtgccgcc cgatacggtg
     7321 cttgcggtcg ggatcgccgc ccccgcatca agactccatt cctcgccagt ccgcgaggcg
     7381 ggcgggcgcc ctaggcgcaa agataacggg accaacggcc gcggagagcc tacctttgcg
     7441 acggagcgac catcgatcga tcgggctgct cgcgcgggcg ggctgacggg gtgcctcctc
     7501 cggctgatgg cgccgggtcg aacccgctac gcctttgtca gtacgcagct cagatccagt
     7561 gcgcatgctg caggcggcga attctcggcc cggcgcccca accagctcga cgagcggatg
     7621 tgtcacgacg acatccttgg cgagctgccg ccgggaggct aggcggtttt gttcagcagg
     7681 cgatctccga aataaaggac ccggtccggg gacgcgctgc cgtcatcgta aagcgcaatg
     7741 atctcctccg gcaggtgctg cagatccgat gcccgcagat agtcccggat ggtcgtgccc
     7801 gtggagcgcg aatcgagatt cggtgcgcca tggtacgccg ccagatcctc gaggtgaacc
     7861 gtccgaaaat cgatgctgta gcgcgccagg ccggtcgtgt tgggcaccgt ctcgtgcagt
     7921 tgcgcgcccg agaacacgat gacgccgccc ggcggcggca ggtagcggat cgtgatcggc
     7981 tccagctcct gttgcggctt tggctgctcg cggctgtcgc ccctcacatg ctgcgcggcg
     8041 ctcgcccggt tcttggtatt ccattcgtaa tagttgtaga tctcggaatt gttcttgacc
     8101 ggctccgtgg catagcgggg atagaagccc atcgcgttgt tcgggtccag gggatagatc
     8161 gggatccacc agttgatctg gcacatcggt gccgagtacc aggtatcgcg atgcggatgg
     8221 aacgcatagg cgatcccgga tgacaggaag tgcgagggat acgccgatcg caggcgcggc
     8281 acgtcaaaat agagcttgtc gacgtggacg ccgttctcgc tcatgatggt cgggatgaac
     8341 tgcttgcatt gcggatgatg aatgaacttc ggcttgagtt cggcgaggat tgccgcgacc
     8401 tggtccgggg tcatgtgctc gtggaccagg cgcggatcgt gcggcgcgaa ggcttcttcc
     8461 agcatggctt tggccaacgc cgccagagcc gttgtgcctg gcgtcggaga gaggatgacg
     8521 atctcacccg catagatgcg gcgacgccgc tcatcatcgc tgatgtcggg atcgaaaaag
     8581 acggaaggca ttttggtgtt ccccttcgtt tttcgtgtgt tgcgaggcgc gaacatcgca
     8641 accacgaggg cgactcgccc tcgtccgatg tggaaggact gcgcggcatc cgctcagccc
     8701 tcggatttca cacagactag gaaaaccacc cgttgtggtt tcatagcccg ctgctcaact
     8761 atagcagtct gtaacagtcc gagagcttcc ctttgcgagg gagctaccat cgcgtgatcg
     8821 agctaccaag gccgcgtccg ccctaagtcg ggggcgctcg cgttctacca agatgttggt
     8881 acgggcgccg ggctgccaca ggttgtagac tttcagcaag catgcgtaag cggtcgctcg
     8941 atcgccagca attcccccgg atcctgaagt aatccggtcc gaggtgcgtc gccgagaggc
     9001 catgtgtcgg ccgcagccat ggcgcacgac ggcagtcgac ctggaaggaa ccgaacagcg
     9061 tggtcagacg tgctctcgca atggctgccg gtgaacagta tctccggctg ttgatcaact
     9121 tcgtcttgat tgccaccatt tcgcgcctgc tgacccctag tgagttcggc gtttccgtga
     9181 tcggcaccgg aatcatggca atcgcgctag gactgcgcga gtttgcgact tcagatttcc
     9241 tgatccagcg tcaggaagtc gcgcgcgagg acgtgcgaac ctctttcacg ctcgctttcc
     9301 tgctgacggt cctgatcacg gtcgccatgt tcgccctcgc gccctggttc gggaccctgt
     9361 acggggagaa cggtctggcg agcttcgttc ggatcgccgc cgtgtccgga ctggtcgagg
     9421 ccgtctcgct gcccatccga ggcctcttgc gacgcgacct ggaattcggg accctcgcca
     9481 tcgtcaatac cgcgagcgca atcacgacgg ctgcggtgac gatcgtgctc gccctcgccg
     9541 gtttcagcta tatgagcgtt gcctgggcga agctggcatc cgcagccacg accaccgcgc
     9601 tgtcgttcta tttccgaccg gacctgtcaa tcctgcgccc ggccgttgtg tcttggggca
     9661 gcgtcctagc gttcggcggc tatagcggcg cgagcttcgt gatcaaccgg gcctacgagt
     9721 cactgcctca gctggtcctc ggccaactgc tgccgcattc ggccgtaggc ctgtacaacc
     9781 gggcccagac cgtctccgac atccccgaca gaatcgtcct gaccagcgtc ttctcggtgg
     9841 cgtttcccgc tttcgcggcc gagattcgaa gtggccgcag cctgaaggag ccgtatctgc
     9901 gagcgctcgg ctacatcacc gtgttctact ggccggcgct gatcctcttg acgatcctgg
     9961 ccgacccggt cgtctcgcta tttctcggcg cacagtggcg cagcgtgaca cctctgctcc
    10021 aggtcatggc gatcgccggc ctggcttggt tccccgtact tctgacctca ccggttctgc
    10081 tcgccgtcag tgccaaccgg gaccgtgtcc tggccgatct cctgggacgc tcggtctccg
    10141 ccgctatcct gtgcgcctcg gcttcgttcg ggatcatggc gatggcggtg agcaagctcg
    10201 tgacgctccc cttccagatg atcatttcct tctgtttcgt acgacgacac atttgctttc
    10261 gatggagcga agtagcggtc gcgctctgga ggagcgccgt cgtcatgctc ggcagcgcgg
    10321 ccgggcccgc ctgcgtggtg gcgttttccg atgggggttt cgatctctcg atcggagctt
    10381 ccctgctggc aatgttgctg gccgtgatcg gctggctgac ggcggtgctc gccgtccagc
    10441 accccgtgct gctcgagctc ggcagagccc gcgatcacct tagcgaagtt ctgcgcccga
    10501 agctgttcgg gttgccgcga gcgggtcacg gcgcccgtcc agaggggcag ggggcccgga
    10561 ccgtctagct agcaagcccg gaattcggcg cggtctatcg tggggcataa ccgccggagg
    10621 acgccgtgga tgcggggccc ggctccagtt cgctcagctg gcgctgctcc ccggcggctg
    10681 cgcggtgagc ttccttcagg agaccggcac ccgaaaatcg ggccagctgc ggtccttttc
    10741 cgagatctcg gtcacaggca aaggccagtc gatggcgaag gccgggtcgt tgtagcgcac
    10801 cccactggcc gcctggggtg cgtagaacgc ggaaatcagg tagcccacct gcgtgtcgtc
    10861 gcagagggtc tgaaacccgt gggcgaagcc ctcgggcaca tagagctgaa gctggttgtc
    10921 ggccgtgagc tcgaacccct gccagtggcg gtaggtgggt gagtccggac gaagatcgat
    10981 gatcacgtcc cagatcgcgc cttttaggca gcgcacgacc ttgacctcgg catgcggcgc
    11041 tcgctggaaa tgcataccgc gcagcgtgcc ccgggtgacc gattgagagg tgctgtgctg
    11101 aacgaagccg gtcgtgagcc cctgatcggc ataggcctga acgcagaacg tgcgcgcaaa
    11161 gaagccgcga tggtcgcgcg ccgggatcag atgaatcagc caggcgccac tgaggttggt
    11221 ctcttcaaag cccatgggca ggctccgaaa tcgaagtgtg cgggaactcg atcaggcggc
    11281 gtgcgccgag tccaggcagt ccgagattgc gtccgatccg ggcaccgccc ggcaggaact
    11341 cagtcatcga ccaccgcgct gaacaggagc gagatcgtac ggcgcacgct cacctatccg
    11401 atccgcctct gccgcctccc gattttccta ttcggtaccg ctctcgcaga cgcgccgaca
    11461 agtcgagcaa acggggtaat cggcgtgaca aagggcgact cgctcctatt cagtaggagg
    11521 cgaaggtttc gcgccggaag atggcggagc ctcgccgccg tcctcgacca gcaggccgct
    11581 cgttttcagc aaatgggcgg cgtctgcgat gacgccgaac ggcagtttgg cacgctcggc
    11641 gatatcgagg aggcaatggc ggccgtcggc gagattgagg atccacagga acgccatgct
    11701 cttcgccgcc gcctcgggat ccccgcccag ggcgccatag aggccgcggc ggccgagctg
    11761 ggcttcacat ttgggtgtgg tgctccgagg ccaccagtcc cgctcgagca cctcgatcag
    11821 gccgacgatc atgcggtagg acgaggccag atgctccggt ccgatgaagt caagattgtc
    11881 ggctgacgtg tggtattcgg ggaaagtgcc gaactgactg cgctggaaca gtccaaccgg
    11941 caggttgaac cccggcgagc agtattgccg ctcgtcatat ccgtagggga agaagtcgag
    12001 aatggtcggc gacggcgcgg catgctgcag cacatgcgcc atcgcccggt cgatgaaggc
    12061 gtcgccgcgc cggcttttct tgtaagtcgg cccgccggcg tcgccgacgc aggacagtac
    12121 gagcccatgc cggatgcgcg cgagccgatg ctcgttgcgg gcgagccaag cgattgcccc
    12181 gatcgtgccc ggagcgaaca cgaaacggta gctgtagcgc gtctccaagg aggcaagacg
    12241 ggcggccaga cgcgtcagca gcgccaggcc cgaacaattg tcgttggcga gcgaggggtg
    12301 gcagatgtgc gccgaaaaga gaacctcgtc gtcagttgca ccccggtgca gatactcgcc
    12361 gtaggtcagg ctgccggcat caaggctggc atcgatcatg acctcgtaga cgccttccgg
    12421 aagtgccgcg aggtgagtgt gggccatgca gaagccccac cgctcggcgt aatacgaggt
    12481 ccgatatggg atgagatcgg gctggtcggg cagcgtgaaa acgtgctcct tcagctcatc
    12541 gagcgaaatc ctcgcatgca ccggccggct gtagttcagc acgtgcagat tggaggcctg
    12601 gaagtcgacg atccggcacc cgctcggatc cttgatgtaa gcatcccgga tcgtccactc
    12661 cggcgggacc gaccagtcga agacctgggt accggtcggg acctcgtgct gctcgagttc
    12721 gacgtgccgg gccagccgct cgagggtttg gcgaatgccg tcgccggtga tgctgcggca
    12781 gatcggaaag atctcggcgg ccagggcaaa gatctgatcg ccgatgctcg ggtcatcgag
    12841 cagatgcttc accgactcga ccgctgccat cgagcgttcc cgaggccggg cgccgaccgg
    12901 atcaggccgt agcggccgcc gctagcgcga actccggggc gggcagatcg aggcggcgaa
    12961 gatcgtcgcc gacccttccc tcgcgcatca acatcttgat ttggctgatc cgctgatagc
    13021 ggcgctcgat ctgctcgagc gtgaggcccg cggcgcgata ggcggcgtgg agctgctcgg
    13081 cgcccttgcg gacgtcccat cgtggctcga actcgggcag gacgcgggcg atcttgtcga
    13141 agctcacccg ataggaccgc ttgtcgggtc cggcgtccgg cgcgatctcg agccggcagc
    13201 ccggaaccac ttgcgccacg acctcggcga tctcgcggat gcgatagttg tgctcggtcc
    13261 ggccgacgtt gaacgcctcg ttgaagacct tgtcggccgg tgcctccagg ccggcgagga
    13321 atgcgcgcgc gatatcctcg atgtgcacga tcggacgcca gggggagccg tcggacttca
    13381 ggtagatcag tcccttggtg accgccgagg cgaccaggtc gttgagcacg atatcgacgc
    13441 gcaggcgggg tgatagacca taggccgtcg ccgggcggag gtaagtggga cagaagccat
    13501 cgccagccaa gcgggcgatg tcgcgctccg actgcacttt cgagcgcccg taggccgtga
    13561 ccgggttgag cgcgccggtc tcatcgatca tgccgtcacc ggcgagcccg taattgctgc
    13621 aggacgacgc aaacaggaaa cgcgcgaccc cggcgcgttt cgccagcgtc gccagacgaa
    13681 cgcttgctct atgattgatg tcgtatgtga tctccggatt gaggtccccg agcgggtcgt
    13741 tcgagagcgc cgccaggtgg atgacggcgt cgaagcccac cagatcgtcg accgtaacgt
    13801 cgcggacgtc cttgcggagg ctcggcacct cggcgatcga gccgccgatc gcaaacgtgc
    13861 accgctcgta gagatcgctg tcgtaaccgg taacctcgtg gcccgcgtcg agcagcatcg
    13921 gcaccatcac cgtgccgatg tagccacggt ggccggtcac cagaactcgc atcgatggcg
    13981 ctcctcactt gcggtggaga tcggccggtc gccccgacgc gatcgatgcc cgactcggat
    14041 cgcacaagcc taaagccaaa agcgcggacg ctgccagaga tccgatcggc ggacgtcgct
    14101 gcgaccatcg gggtagacta ccttccgacg gctgaggggc gcgcggtcgc cggtgttttc
    14161 tgccgaccgg cgcttgaccg agtcggcatg cgccagatgg cgggtcgaga tcaccttgcc
    14221 gcgatctcgc tcgcgactgc ggacggtcgc tggggcggtc cgtcgggatg attgtagcgc
    14281 cggtacacgg tctgggatgg ccggccgacg agatagcgct cgacgccgtg gtcgagggcc
    14341 agccgataga cctcgagcgc gccgttgaac gcggccaccg tgagatcgat gtcccggtcg
    14401 gtgtgggaat agctcacgac cagcgagggc atcagcacgc cacgccggat cgtctcctgc
    14461 aggaacagcg tccggaacgc ctgcgagggc cgtccgtccg gccccagcgt ggcgtaggac
    14521 aggcagcatg gcttgcccag gatctgcacg aattcggcca ggccgtgccg ccggatggcc
    14581 tgctcggcct gctcccggag ccgcgtgccc tgccgataga ggtgctcgat cacgttctca
    14641 cgacgataga cctgcatggt cgcgatcgcc gcggcgagcg cgtgagtctc ggcgccatgc
    14701 gtggtcgaaa gaaggaaaac ccgcggccga tcgcggtggt cgagaccgcc caggcgcatg
    14761 aactcgcgcc gacccgcaag cgccgagacc gagaagccat tggcgagcgc cttgccgaag
    14821 gtggcgaggt cggggacgat gccgtagagc ttctgcgcgc cgccgttgtg ccagcggaag
    14881 cccgtgatca tctcgtcgag gatcaagagc gcgccgttgg catggcacag ccgctgcagg
    14941 gcctgcagat agcccagcgg cgggtcctcc gtccgtgccg gctcgagaat cagcgcggcg
    15001 atccgaccgg gatgctgctc gaacagcgcc tcgaccccgg cgatgtcatt gtagtggaag
    15061 gtcagggtca gctgccggat tgcttccgga acgccggcgt tcatcggcgt ggtcccgatg
    15121 aaccagtcgt cggtggagaa gaacgggtgg tcggcgcagc aggcgatcag gtcgcggccg
    15181 gtgtaggcgc gggccagccg gaccgcgccg gaggtggcgt ccgatccgtc actgcagaac
    15241 ttcaccatct cggcgccgtc gaccagctcc aggaaggcct cggcgcaggc cggctcgatc
    15301 ggcgacgggc gggtgaagtt gctgccgcgc agcagttcgc gctgcacggc ctcgaccacg
    15361 gggggatagg cgtgaccgag gccgacggcg cggttgccca tcccgtactc gatgtactca
    15421 cggccgtcca tgtcccagat ccggcagccg gagccgcgcg cgatgaatgc cggcgcgagg
    15481 atcggatatt gatcatcacc cttggcgtag gtgtgacacc cgcccgggat cagctgatgg
    15541 gcccgcgcct ggagggacgg cgaagcctgc gctgagcgca atcccaattg gtgaacctga
    15601 tgaccattca gctgcatccg gccgtccccg aacagttggc gacgtctccg aggccgccgg
    15661 gccgttttgc ggagcgtcgg gagcgccccc tcgcaggacc tcagagagtc gggccctgtt
    15721 gcgggtgggc cggcgcttcg ccgcagaccc gccggtgatg cgccggttcc ggaaaactac
    15781 cgcaagtccg cagctgtgcc cggtctgcaa aacggcctag agccgggcaa gaaagtcgta
    15841 gcccctgcca accggccgat cccatctgac gcgacgctgt cccaaaagcg gcaagcgccg
    15901 gcggtccggc catagtggtc ggcccgccgg tcaggtgccg tgccggccgg ctagacgcgc
    15961 agatcgggac ctgaagtcga ccggcggatc gtgtcagccg ggcgacgatg gtgaccgccc
    16021 tcttcgaaaa gcccctgagt caggtacacc acctgagtcc gattggttgc tttgagcttt
    16081 ttcatgatat tgcggatgtg aactttgacg gtgctttcac acatggtaag ctcgtaggcg
    16141 atcagcttgt tcgccatacc gcgtcgcagg cagtccaaaa tctgcgcctg gcgctgcgtg
    16201 aagccctcga tcagcggcgg actgtcggat tcgtcgccag cgcccgcaga ggacgacagc
    16261 agggcggtgg ccggtgcaaa ggtaccgccg gcgcagatca gatggaccgc ctcgacggcc
    16321 accagcgagg ccaggctcgt gggaatgtag ccacgcacgc cgagctcaaa cgccgcgcga
    16381 atgttctcgg catcctcgta gtcggacagg accgcgaccg gaaccgaggg catcagctcg
    16441 gcaacgcgcg ccatcaggct cgcgaccccg cgcgacgaca tgcgctcggg gcccgtggcg
    16501 atgatcacca cctggatctc gttcccgaga tccggcgcgg tcaggatctg ctccggatcc
    16561 ggcaaggcgc ggatgctgaa cccgctgagg tggctctcaa gccagcgtcc gatgcagtcg
    16621 cgggtgaagg tgcgcgtgtc gaggtagatc agggtcgcgc cgctggcggc cgcttcgccg
    16681 gcccccggcg gcgcggcgtc cgagcgtgct cgtcgcgcgt ccagatcgat cgacttcagc
    16741 ccgaattcgt ccagcgatgt ctcagcgatc gtgccgccga cgcgcagccc gatctcgctc
    16801 cagccgagac cccggctcgt ggcctcattg gtgttcatct cgcggcgctc tcctgtggcc
    16861 tttaggctct ggatttggat aaactccgtc agcccggcac tgcccggact ccggcgttca
    16921 ctaacgcgtt cgtaggagtt tggttcccct ccattgttag taacatcagc ccaatcagca
    16981 tggtctggac gccggagaat cgcctacccc cagatggggg tgattagatt caaatttgtg
    17041 ctataaggag gtaccgaaat cgagagcgcc ctgtctagaa ggcgccggga aaatcaggag
    17101 cgcacatgcc ggttgaaaaa gagctagagg ggctcatacc tttaatttac gacacgacca
    17161 cctctgccgg ggccctgcca gaactgctcg cagttctgca gcaattgttt ggcgcccgag
    17221 cctgcgcctt ggccgtgttc aacttccagc gtcgagaagg cgcactggat gccagcagtg
    17281 gctatcaccc ggagttcatt tcgtcgtacc gcgactacta ttcacgtcac gacccttggt
    17341 tgcgcaatga gcaggcctac cgagtacctg gcacagtcca gctaggtcag gaaatcgtcc
    17401 ctacggctga actcatcact acccgattct atcaagagtg gctaagaccg caaaatcttc
    17461 accaacgttt gagcacggtg ctgatccgag aagaggcaaa tttggtttat ttcgctacgt
    17521 tgcgtggacc ggcggacacc atgttcggtc ccaacgagat tcggctgtgt cgggctctgg
    17581 cgccccatct gcgcagcgcg gtccagatgc ggcggcagct ggcgatgctc caggtcgagc
    17641 ggaatgcggc gctcgaggtt ctcgaccgac tgccgactgc ggtcgtgctc tgtgaccaga
    17701 gcggcacgcc gatcatcatc aacggcgccg cgcgcgagat ccttgcgagc aaagacgggc
    17761 tcgtggtccg gggtggccgg ctcgcggctc gccggcggct cgagaccgcc gctctcgaga
    17821 acctgatcgt gggcgctgcc agcgccatcg gagaaagcgg gagctccggc ggcacgatgt
    17881 cggtctcacg agcgtccggc tcgcggccgc tgtccgtgct ggtatcgccg atgcgagcac
    17941 ccggcgcggt gcctggattt ggccagatcg ccgcggcgct cttcatcagc gatcccgaat
    18001 cggtcatcct caccaacgag gaccggctgc tgcacctcta cgggctgaca cctgccgaaa
    18061 gtcggttggc agccaagctt gcccagggcc gctcgctcga ggaggcggcg gccatgctca
    18121 acatcaagat ccagaccgcg cgcagctaca tcaagcgcat tctgaacaag accggcacga
    18181 aacggcaggt cgagctggtt cgcctcctcc tcctcagccc cgcccacctg agctgaaggc
    18241 ggcccgtttc gccgaccggg ccagccggca gacccacacc tgggttgtaa agccggcccc
    18301 ggtcttccgc tcatcggctg aagacccagc aggctatgcc gggcggagcg cgacgcccgc
    18361 gggcaggccg cggttcgatc cgagcaagcc accctgatgg cggcgatcga ggttcggaaa
    18421 tcggttgacg gcgggccctg gatcgtcgag agaaacgccg gccgtcagtt cggaaaccaa
    18481 gtgggaacca tgcgctggcc ggagcccttg attcccggtc gtctggtgcg gcgctacaag
    18541 cgatttctgg cggacgtcgc cctggaggat ggccgccagg tcaccgtgca ttgcccgaat
    18601 cccgggcgca tgctcgggct cgatgcgccc ggagcgcagg tctggctctc acgctccgca
    18661 agcgcctcga gaaaactgcc gctgaccctc gagttggtcg aggttgccga cggattggtc
    18721 gggatcaatt cgatgcatcc gaaccggctg gtcgaggagg cgctgagggg cgggctgatc
    18781 gccgagctcg caggctaccc gagcatccgg cgggagatcg cctacgaccg agggtgccgg
    18841 gtcgatttcc tgctgtccgg cggagggcgg ccggattgtt tcgtcgaggt caagaacgta
    18901 catctgaagc ggacgctggg ggcggagttc ccggacaccg cgaccgcgcg gggagtaaag
    18961 catctgaagg ctctcagccg ggcggctgcg gccggcgccc gcgcggtcgt gctgtacgtg
    19021 gtccagcgga tcgactgcgc agacttccgc ctcgcgagcg acctcgaccc gggctatgcc
    19081 gcggccgcgc gcacggctgg aacggccggc gtcgagagcc tgtgccgggc gtgcgcgatc
    19141 acgctcgacg agattcgcct cgccgccgcg ttgccgatcg tcccctgacc gtgatgagcg
    19201 cctctgatga tgcaggacca gctacacacc gccaaacgct ccgccagcga ggttcaggtt
    19261 ccgctgcacg atgcgacagg cttcgtcggc atgggtcggg cggggcagct cgccgccgcg
    19321 gtgctcgaca tgatcgaccc gcacgtccgg ccgagggtga gcacgggcga actcgaccgc
    19381 ctttgccatg ctttcatcct cgaccatggc gcggcccctg cacccctgaa ctatcgcggc
    19441 tttcccaaat cgatctgcac ctcgatcaat catgaggttt gccacgggat tccgagcgaa
    19501 ggccgcgttc tgcgcgatgg cgacatcgtc aacatcgacg tcacg
//
LOCUS       DATAOZ010000124         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3206338, whole genome shotgun sequence.
ACCESSION   DATAOZ010000124 DATAOZ010000000
VERSION     DATAOZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206338"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VHR64_03520"
     CDS             complement(<1..1097)
                     /locus_tag="VHR64_03520"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX3301924.1"
                     /translation="MTATIFVTDDEPAIRSALVKRLSRRHHRVLAFESGEALVDALDH
                     EVPDLMLLDVKMPGLSGLDALKACRAKAPFALVIMLTAYGTVQDAVEAMKLGAYDFVI
                     KTVDLDGMEQVLDRALELFALRRRLSYQSEQDRNQYAMDHLIADSPAMKSLLEQIREV
                     AHNPKSTVLMLGETGTGKEFVARVLHHNGSRGSGPFVGVNCTAIPRDLFESELFGYER
                     GAFTGAHQRKLGLLDRAEGGTLFLDEIGDLDLSMQAKLLRVLQERTFRRLGGTADIAA
                     DFRLIAATNRDLKKDIAAGVFREDLFFRLNVVALELPPLRHRSEDIYPLSMKALLHYG
                     KEFGKEVAEIDVEARGLLERYHYPGNIRELQ"
     gene            complement(1094..>2825)
                     /locus_tag="VHR64_03525"
     CDS             complement(1094..>2825)
                     /locus_tag="VHR64_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3301925.1"
                     /translation="VDLETGFRGFVITQHEKYLYPYRTAQEHIQAVGLSLDEQVRRYD
                     EQLAILSSVRTLVRQFITEKEALIEAMKAGRREEARRYIEEGRGRMIMVQIREDMGRF
                     DKARQQTLNTLLSRLSQDRSTMLSVILGGGTLALCLMLLGLHLIARSITGPLATLATV
                     VGSSPAGIAPTVPVMDREDEIGNLTKVMHTMSSQLRAHLAMVEKSEAELRSVNRDLSA
                     SEAKYRSLVDLAPFGIFSTRGFDITFSNRYNRILAGLDPDGDDNPDAFRQWIHPEDRE
                     RVLTEFARAVQDRKPYETVFRFLHQNGTMRKVLSRRIPLQQEAGQPIVYQGFNIDITA
                     LDDMQQQLSRAERLATLGQVAAGIAHEIRNPLVGIGSTAALLRDEFEPSDEKRADLDI
                     ILNETRRLDRIVNQIIDYARPRDLVPTTWPVDGIIEDVLKLLDTRLESQRIRVVRSCP
                     AGLAADADRDQIKQVLLNLCHNSLDAMPQGGELRLAAGESTRGGHPGIFIEVADTGSG
                     IAPKHLAQVFQPFFTTGKQHGTGLGLAICRNIAEAHRGDIALTSEPGHGTTARLWLPL
                     RPGDRLETAV"
BASE COUNT          513 a          835 c          894 g          583 t
ORIGIN      
        1 ttctgcagct cgcggatgtt gccggggtaa tgataccgtt ccaataggcc gcgggcttcc
       61 acatcgatct cggccacctc ctttccaaac tccttcccgt aatgcagcag cgccttcatg
      121 ctcagagggt atatatcctc gctccggtgt ctcaacggcg gcaattcgag agcaacgacg
      181 ttcagccgga aaaaaaggtc ctcccgaaac acgccggcgg cgatatcctt cttgagatcg
      241 cggttggtgg cggcgatcag gcggaaatcc gccgcgatgt cggccgttcc acccagccgc
      301 cggaaggtcc gttcctggag cacccgtagg agcttggcct gcatggacaa atccaaatcg
      361 ccgatttcat cgaggaacaa ggtgccgcct tcggcgcggt cgagcaagcc gagcttccgc
      421 tgatgcgcgc cggtgaaggc tccgcgctcg tagccgaaca gttcgctttc gaagaggtca
      481 cgcgggattg ccgtgcaatt gacgccgacg aacggaccgg agccccggct gccgttatgg
      541 tgcagtacgc gggcgacgaa ctcctttccg gtaccggttt cgcccagcat gagcaccgta
      601 ctcttcggat tgtgggcgac ttcccggatt tgttcgagca ggcttttcat tgccgggctg
      661 tcggcgatga gatggtccat cgcatattgg ttccggtcct gctcgctttg ataagaaagg
      721 cggcgccgga gggcaaacag ctcgagagcc cggtcgagga cctgttccat cccgtcgaga
      781 tcgacggttt tgatcacaaa gtcgtacgcg ccaagcttca tagcctcgac ggcgtcctgc
      841 acggtgccat aggcggtaag catgatgacg agggcgaagg gggccttcgc gcggcaggct
      901 ttgagcgcgt cgagtccgga gagtcctggc atcttgacat ccaacaacat gaggtcaggt
      961 acttcatggt ccagggcgtc gacgagcgcc tctccggatt caaacgccag gacgcgatgg
     1021 tggcgccgcg acaggcgctt gacgagcgcg gatcggatcg ccggctcatc gtcggtgacg
     1081 aagatcgtgg cggtcatacg gcagtctcca ggcggtctcc cggccgcagc gggagccaca
     1141 aacgggcggt cgttccatgc ccgggctcgc tcgtcagggc aatgtcgcct cggtgcgcct
     1201 ccgcaatgtt gcggcagatc gccagtccga ggcccgtccc atgctgcttt ccggtggtga
     1261 aaaagggctg gaagacctgc gccaggtgtt tcggggcgat gcccgagccg gtatcggcca
     1321 cttcgatgaa gatcccggga tgtccgccgc gcgtcgattc tccggcggcg aggcgcagtt
     1381 cgcccccctg gggcatggca tcgagcgagt tgtggcaaag attcaacaag acctgcttga
     1441 tttgatcgcg gtcggcatcg gcggcgagtc cggcaggaca agagcgtacg acgcggatcc
     1501 gctggctctc caagcgcgtg tccaacagct tcagcacgtc ttcgatgatg ccgtccaccg
     1561 gccacgtggt cggtacaaga tcccgcggcc gcgcgtagtc gatgatttga ttcactatgc
     1621 ggtcgagccg gcgtgtttcg tttaaaataa tgtcgaggtc cgcccgtttc tcgtccgatg
     1681 gctcgaactc atcgcgcaaa agcgcggccg tcgagccgat cccaaccaac gggtttctga
     1741 tctcatgggc aatgcccgcc gcgacctggc cgagcgtcgc gaggcgttcc gcgcggctga
     1801 gctgctgctg catgtcatcg agagctgtaa tgtcgatgtt gaatccttga tagacgatcg
     1861 gctgaccagc ttcctgctgg agcggaatgc gtcggctgag caccttacgc attgtgccgt
     1921 tctgatggag aaaccggaag acggtttcat agggcttccg atcttgcacc gcgcgggcga
     1981 attctgtcag cactcgttcg cggtcttccg ggtgaatcca ctggcggaac gcgtcgggat
     2041 tgtcgtcgcc gtccgggtcg agaccggcca ggatccggtt gtaccggttg ctgaacgtga
     2101 tgtcgaatcc tctggtcgaa aaaataccga agggggccag atccaccagg ctacggtact
     2161 tggcttcgga cgcagacaga tcgcggttca cgctgcgcag ctctgcttcg gacttctcga
     2221 ccatcgccag atgagcacgc agttgactgc tcatggtatg catgaccttt gtcagattcc
     2281 cgatttcgtc ttcccggtcc atgactggaa cggtcggcgc aatgccggcg ggggatgaac
     2341 cgacgacggt tgccagcgtg gcaagcggac cggtgatgga gcgggcgatc aagtgaaggc
     2401 cgaggagcat gaggcatagg gcaagagtgc cgcccccgag gatgaccgac agcatggtgc
     2461 tccggtcttg tgacagtcgc gagagcaggg tgttcagagt ttgttgcctc gccttgtcga
     2521 agcggcccat gtcctcacgg atttgaacca tgatcatgcg gccgcggcct tcttcaatgt
     2581 accgtctcgc ctcttccctc ctgccggctt tcatcgcttc gatcagcgcc tctttttcgg
     2641 tgatgaactg cctgaccagg gtgcggacgc tcgagaggat cgcaagctgc tcgtcgtatc
     2701 gcctgacttg ttcgtcgagg gaaaggccga ctgcctggat gtgttcctgt gcagtccggt
     2761 aaggatacag gtacttctca tgctgggtaa tgacgaaccc gcggaaccct gtctcgaggt
     2821 cgacg
//
LOCUS       DATAOZ010000125         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1365746, whole genome shotgun sequence.
ACCESSION   DATAOZ010000125 DATAOZ010000000
VERSION     DATAOZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365746"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1278)
                     /locus_tag="VHR64_03530"
     CDS             complement(175..1278)
                     /locus_tag="VHR64_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301926.1"
                     /translation="MNPRLWRVILVALCVTLLCQATLPAVPVRAAVPEITFSVGQGVS
                     AEDEVIVREGIEFAQSYVDDQLAPFPESKITVNVRNTRDTTGGNAAAFSAGDYIVVFT
                     RTPGWKTLAPFDRIHVVAHEYIHSWQRATAGQEGETAPLWLIEGSAEYLAYDAVTRAG
                     LVRGREVIDTQAWYVLDAPKMASLDQLEDRDAYYGEAGPVYSLAFLAVTQLAKEDGPA
                     GISRFFEAMGAGDSWQEAFSTAFGQDVDDFYAAFEDTRADLIAPRDLPDSFLPIRPVH
                     TESPIHLAPLPETAATDEQVAVIGESEPGATCTMRLRSAESGERLTRTTYADGAGRLF
                     WLVTIPSDFGPGTVRLSANCGDESATGSFTVEP"
     gene            complement(1366..>2177)
                     /locus_tag="VHR64_03535"
     CDS             complement(1366..>2177)
                     /locus_tag="VHR64_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301927.1"
                     /translation="TMRIKLAGETGTQKIRLLGVEPPAKGECMSNEAERLLDKNTGKR
                     VLVEEDAAITGGSGSMRYVWLINADGTRTLLNQKLIADGLTQAAAVPDDARFGLWLLT
                     SERAAEEGQIGLWAGCETPEATATTAKPSPSPTPAKATPSATPAPKATSTPAAGSAVK
                     WTGEIGDIGTINFTIADGAMTNFGMRYDIPCTGEVLGPPTGVTSKVLVPPMPLANGSF
                     TLEFPVSGSGGSITVSGSLTDDDSAVGTFTLPPVPECTTEELQFEWAASVE"
BASE COUNT          377 a          703 c          704 g          393 t
ORIGIN      
        1 attccgttgc tcccatcctg caactggagc aaggtcgtca catcggtgag atcacgtaat
       61 cgagttgagg gtggagggac cagaaaaggg tagccagaag tcagtagccg gtagtcagtt
      121 cggaaaacgg agaaccgaaa acgaaagacc agcgaccaaa gacccaaaac ctggtcacgg
      181 ctccaccgtg aagctccccg tggccgattc gtcgccgcag ttggcgctca ggcgtaccgt
      241 gccgggaccg aaatcagatg gaatcgtgac cagccagaag aggcgaccgg cgccgtcggc
      301 atacgtcgtg cgggtcaggc gctcgcccga ctccgcgctg cgcagccgca tggtgcaggt
      361 tgcgcccggc tccgattcgc cgatcaccgc cacctgctcg tcggtcgcgg cggtctccgg
      421 cagcggcgcg aggtggatcg gactctcggt gtggaccggc cggatcggga ggaacgagtc
      481 gggcagatcg cgcggggcaa tcaggtcggc gcgcgtgtct tcaaacgccg cgtagaagtc
      541 gtcgacgtcc tgaccgaacg ccgtcgagaa ggcctcttgc caggaatcgc ccgcgcccat
      601 cgcctcgaag aagcggctga tcccggccgg gccgtcctct ttcgccagtt gggtgacggc
      661 caggaatgcc aggctgtaga ccggtcccgc ctccccgtag taggcgtctc ggtcctcgag
      721 ttggtcgagc gacgccatct tgggcgcgtc gagtacgtac caggcctggg tatcgatcac
      781 ctctcggccg cgaaccagtc cggcccgggt caccgcgtcg taggcgagat actcggcgct
      841 gccctcgatc agccagagcg gagcggtctc gccctcttgg ccggccgtgg cccgttgcca
      901 cgagtggatg tactcatgcg ccaccacgtg gatgcgatcg aacggcgcga gcgttttcca
      961 acccggcgtg cgggtgaaga cgacgatgta atccccggcc gagaaggcag cggcattccc
     1021 tcccgtcgta tcgcgggtat tgcgcacgtt gaccgtgatc ttcgattcgg gaaacggcgc
     1081 aagttggtca tcgacgtagg attgggcgaa ctcgatcccc tccctgacaa tgacctcgtc
     1141 ctcagccgac acgccctggc cgacgctgaa ggtgatttcg ggcaccgccg cccgcaccgg
     1201 cacggccggg agcgttgcct ggcaaagcag cgtcacgcag agcgcaacca ggatcacgcg
     1261 ccaaagtcgg gggttcatcg acctacctct tccagacctg cgggcactcg tctatcaatg
     1321 ccgttggagg acgatgatgt cgtcctccaa tgacgcacaa tgcggttact cgactgatgc
     1381 agcccactca aactgaagct cttcggtggt gcattccggc actggcggaa gcgtgaacgt
     1441 gccaacggcc gaatcgtcat cggtgagcga tccgctgacg gtgatcgaac cgccgctgcc
     1501 gctaacgggg aactcgagtg tgaagctgcc gtttgcgagc ggcatcggcg gcacaagcac
     1561 tttgctggta acaccagtcg gcgggccgag gacctccccg gtgcagggga tgtcatagcg
     1621 catgccgaaa ttggtcatgg cgccatcggc gatcgtgaag ttgatcgtcc cgatgtcgcc
     1681 gatctccccg gtccacttga ccgcgctccc ggccgccggc gtgctcgtcg ccttcggtgc
     1741 aggcgtggct gagggcgtgg ccttggcggg cgtcggcgat gggctcggct ttgcggtcgt
     1801 ggccgttgct tccggggttt cgcagccggc ccagagcccg atctgaccct cctccgccgc
     1861 gcgttcggag gtcagcagcc agagaccgaa gcgggcgtca tccggaactg ccgccgcctg
     1921 agtcaaaccg tcggcgatca acttctggtt gagtagcgtc cgggtgccat cggcattgat
     1981 cagccagacg taacgcattg atccgctgcc gcccgttatg gcggcatcct cctcgacgag
     2041 gactcgtttt ccggtgttct tgtcgagcag ccgctcggct tcgttcgaca tgcactcgcc
     2101 cttggccggc ggctcgacgc ccagcaagcg gatcttctgc gtacccgttt cgccagcaag
     2161 ctttatgcgc atcgtct
//
LOCUS       DATAOZ010000126         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2681815, whole genome shotgun sequence.
ACCESSION   DATAOZ010000126 DATAOZ010000000
VERSION     DATAOZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2681815"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VHR64_03540"
     CDS             <1..984
                     /locus_tag="VHR64_03540"
                     /inference="COORDINATES: protein motif:HMM:NF005928.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX3301928.1"
                     /translation="PTYRVAAFRRAAETISALEPGELEHMASNKLLRSLKGIGEATEA
                     VILQVLAGKVPEYLAKLEAEPQPFAVAGDALRRTLRGDCHTHSLWSDGGSPIIEMAHA
                     ARTLAHEYIVITDHSPNLTVANGLSTERLMRQLEEIEGVNGQLAAEAEAGAPPFRVLS
                     GIEVDINEDGTLDQTPDVLAALDIVVGSVHSKLRSPRDVMTKRMLAAIEDPNMDILGH
                     CTGRIVVGRGRPQSEFDHAAVFTACRDNEVAIEINSRPERKDPPRNLLKLAVDIGCRF
                     SIDTDAHAPGQLEWQYIGCDRAAECGVTEDRVINTSPAGRLMSWSASRREP"
     gene            complement(1119..1295)
                     /locus_tag="VHR64_03545"
     CDS             complement(1119..1295)
                     /locus_tag="VHR64_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301929.1"
                     /translation="MAGPQPAPHDVVCRYENQGSDEVGRFDEQNRQAANAWDRLHALR
                     TDSYSATDYVVVAE"
     gene            complement(1386..>1584)
                     /locus_tag="VHR64_03550"
     CDS             complement(1386..>1584)
                     /locus_tag="VHR64_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301930.1"
                     /translation="GIVEAMSKKLVADGMYFVGIDVVGDKVVEINAESPGGMQSVERL
                     YEIDVCPTVIEALERRTGPKS"
BASE COUNT          313 a          532 c          457 g          282 t
ORIGIN      
        1 ccgacgtatc gcgtcgctgc attccgtcgc gccgccgaga ccatctccgc gctcgaaccc
       61 ggcgaattgg aacacatggc cagcaacaaa ctgcttcgct ccctgaaagg aatcggtgaa
      121 gcgacagagg ccgtgatctt gcaagtactg gcgggcaaag tccccgaata cctcgcgaag
      181 ttggaagcgg agccgcaacc gtttgctgtt gccggcgatg cgctccgcag aacgctacga
      241 ggtgactgtc acacgcactc gctctggtcc gacggcggca gtccgatcat cgagatggcg
      301 cacgccgcgc gtacgttggc tcacgaatac atcgtcatta ccgatcactc ccccaacctc
      361 accgttgcca acggactctc gacagagcgg ctcatgcgac aactcgagga gatcgagggc
      421 gtcaacggtc aacttgccgc tgaagcagag gccggagcgc caccatttcg tgttctcagc
      481 ggcatcgagg ttgacatcaa cgaggatgga actctcgatc agaccccgga tgtgctcgcg
      541 gcactcgaca tagttgtcgg cagcgtgcat tccaaactgc gttcaccccg cgacgtgatg
      601 accaagcgca tgctcgcggc aatcgaagac ccgaacatgg acatcctggg acattgcacc
      661 ggtcgcatcg ttgtcggccg tggacgtccg cagtccgagt tcgaccatgc agccgtcttc
      721 accgcgtgca gagacaacga ggtcgcaatc gagatcaact cccgaccaga gcgcaaagac
      781 ccaccgcgca atctgctcaa gctggccgtc gatatcggct gccgtttctc aatcgacacc
      841 gacgcacacg caccgggtca gctcgagtgg cagtacatcg gctgcgaccg cgccgccgaa
      901 tgtggtgtca cggaagaccg ggtgatcaat accagtccgg ccgggagatt gatgagctgg
      961 agcgcatcgc gccgtgagcc ctgatttggc ccccaaacgc caagtccatt aacgcgaagc
     1021 ttggtgccaa gtggctgccg gacccttgag ccccggcccg gaccactctc gcgggtcggg
     1081 ccagcgctca gtccaacacg ctccttgagg cggcggcgtt actcggcgac cacgacgtag
     1141 tcggtcgctg agtagctatc ggtccgcaaa gcatgcagcc ggtcccaggc attggccgcc
     1201 tgccggttct gctcgtcgaa acgaccgacc tcgtccgagc cctggttctc gtagcgacac
     1261 acgacgtcgt gcggggccgg ctggggacca gccaaccgaa tccctcgtgt ggacgctgag
     1321 aagttcagcg tacgaatccg cgaccccttt tacccggccg ccagctcccc cgatggcgca
     1381 attgctcagg acttcgggcc ggtgcggcgc tccagggctt cgatgacggt tgggcagacg
     1441 tctatttcgt agagcctttc gacgctctgc atcccgcccg ggctctccgc gttgatctcc
     1501 accaccttgt cgccaacaac atcgatgccg acgaagtaca tgccatccgc cacgagcttc
     1561 ttgctcatcg cttcgacgat cccg
//
LOCUS       DATAOZ010000127         6800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3996829, whole genome shotgun sequence.
ACCESSION   DATAOZ010000127 DATAOZ010000000
VERSION     DATAOZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3996829"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VHR64_03555"
     CDS             complement(<1..977)
                     /locus_tag="VHR64_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX3301931.1"
                     /translation="MGAVQRLATVVVLGLVALASVLILYLADEPNRLESQVEAQQHDA
                     VERATNTYLTQCLACHGPAGEGYTAPGEAGTGRIGAPIGGDTSNRVLNQTGIQQTGDN
                     AGKPWSDPNNPQYGSGLEGRANFIRNRVHTGLKAANGTVLMPAFGQEYNGPLNDSQID
                     ELVIMIQHVDWNEVYNKAIEQNGGYPTPPPAAQPTTAATPAGGAASPAVGGGTTFEID
                     MVDVAFQPTELTIPANTDVTINAINKGALPHTFTINNVEDTGEVPAGSSKEVKINLPP
                     GEYDFHCAVPGHAEAGMVGKLIVKEGAAGGGAAATPAAGGGATPAPAAGG"
     gene            complement(1017..1589)
                     /locus_tag="VHR64_03560"
     CDS             complement(1017..1589)
                     /locus_tag="VHR64_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX3301932.1"
                     /translation="MTSVVRTVAGVLGARAVAHLTRAHRQDPAAVPKKLGRRSFVRNA
                     ALGGVVLNISLLTGGLIRFLWPNKTGAFGKTLRVPASLIPPVDGQPYVDSAGKFYLVH
                     TQDGVMALYWRCPHLGCTVPPWNPSEKAFHCPCHGSIYNYEGVRTGGPAPRPMDYMTT
                     TVDSSTGDLLVNTGEINLRQDYQPSQATPV"
     gene            complement(1632..2492)
                     /locus_tag="VHR64_03565"
     CDS             complement(1632..2492)
                     /locus_tag="VHR64_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301933.1"
                     /translation="MAEITYEPKGAGPAAQIDEAKRQKLLELVRGRALARPTDLAEDE
                     VMVWPSLVVVEAVSATVFLGLLTLLSIFVSAPLTEHANTNATPNPSKAPWYFLNLQEL
                     LLHMDAGLAGVIVPTLALVGLAAIPYFDRSPLGVGILGTSAKGRKIAGFSAIWTTVVL
                     TIYIVADEVIGDKRSGWGIGNKIIDWTDEHIGDDFTGVGIIHFIFKPTVSFPEFMGKI
                     VYPTIVMVISIAMLVAAVKLIFRPTRREMIIALFTGFFTAYWVLAIFGTSFRGHGQDL
                     TWPWNLPLHH"
     gene            complement(2526..3254)
                     /gene="extP"
                     /locus_tag="VHR64_03570"
     CDS             complement(2526..3254)
                     /gene="extP"
                     /locus_tag="VHR64_03570"
                     /inference="COORDINATES: protein motif:HMM:NF040969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEX3301934.1"
                     /translation="MRREQTLADRITSSSVWRSVVRHGYPDTQRNQALIIASNVFLHI
                     HPVKIKRYATKVTYTFCLGGLSFFMFLVLVMTGIILMFYYVPSTNLAYENMKDLGSSV
                     TFGTLMRNMHRWAAQGMVIAVFLHMARVFFTGSYKPPREFNWVIGVLLLITTFLLSFS
                     GYLLPWDQLSLWAITVGTNIGGAAPILGPKVNFMLVGDYQIGQATLIRFYTLHVIFLP
                     LAAAFLMAVHFWRIRKDGGISGPL"
     gene            complement(3482..3555)
                     /locus_tag="VHR64_03575"
     tRNA            complement(3482..3555)
                     /locus_tag="VHR64_03575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3519..3521),aa:Val,seq:cac)
     gene            complement(3624..4025)
                     /locus_tag="VHR64_03580"
     CDS             complement(3624..4025)
                     /locus_tag="VHR64_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3301935.1"
                     /translation="MTDQGTNALSIVATEGAPAAIGPYAQGQVIGNLVFTAGQIPLDP
                     VSMDVDGEDIETQTERVMKNLDAVLQAAGSSLSRVVKTTCFLVDLNDFQAFNSIYGRW
                     FGDHKPARSTVQVAKLPRGVLVEVECVAVVG"
     gene            4230..6410
                     /locus_tag="VHR64_03585"
     CDS             4230..6410
                     /locus_tag="VHR64_03585"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEX3301936.1"
                     /translation="MSHTLNAVAGLGALLLIATVIGLSLYALGHQGRIYQGVTVAGVN
                     VSGMTETEAVEAVQRDYSIYMNTPLNLTFEGKTYAISPNELGIRLDAQASVDAAMQYG
                     REGSLWSRSRAWAKGLLTGSDVPAVLTADNSRVDQGLLALTDVVARPATNAWIDFSSD
                     EAKVVPEVPGVGYNYGMTRALVMNRVQTRSSAPIEIATTVVQPGVTAQTLSSTLPTAQ
                     TAVSSALVLQGINGQNWSVDAQQLKSIVSVSSDGTSVTVDRDAVQRLVSGIAESVNRD
                     ATDAVLFVNGDGELEIVPGVNSLAVDVDKTVDDVASSLVAGSHDVEVTIEQKSPAITD
                     ERAAASLEQITKTLATGLTVKWDGGQKQLTPTDLLSSLVIKATPDQADAFAFSLSHQV
                     LSDYIKSFAGDIEIEPHEPTFRLVNGEVKAEKKGQTGVVINYDSSAERIEKAVFSGYS
                     SSNLKVDVIKPKFSNNDAGAIKLPDVLGEAATPYSNSSEARKTNVERAVDLMNGWLIA
                     PGDEFSYVDHIGQVTEDNGFEVGLGIVADPNNPGAVMTAPVVGGGICQVSTTIFQSAF
                     WAGLTFTERHQHPYWINSYGIGEGGMKGLDAMVNIEDEPNEWAITLDMKFVNTTDHWI
                     AIEMTADGDNVTSRILGTNPGWTIDVSDPDITAITKPDPTPIRQDSPEVPSGEERQVE
                     TAQDGFDADIHRVVKDKSGTVLDTYDVSSSYSATSNRILVGTGQ"
BASE COUNT         1363 a         2068 c         2134 g         1235 t
ORIGIN      
        1 ccacctgccg cgggagctgg agtggcccca ccgcccgcag ccggagttgc agcggctccg
       61 ccgccagcag cgccttcctt gacgatcagc ttgccgacca tgccggcttc agcgtgaccc
      121 ggcaccgcac agtggaagtc gtactcgccg ggaggaaggt tgatcttgac ttccttgctg
      181 gatccagcgg gaacctcgcc cgtgtcctcc acattgttga tcgtgaaggt gtgcggaagc
      241 gcgcccttgt tgatggcgtt gatggtcacg tcggtattgg ccgggatcgt cagctcggtc
      301 ggttggaagg cgacgtcgac catgtcgatc tcgaatgtcg tgccgccacc cacggcaggt
      361 gaagccgctc cgccagcggg cgttgccgcg gtcgtcggct gcgcggccgg cggaggcgtc
      421 ggatagccgc cgttctgctc gatggccttg ttatagacct cgttccagtc gacgtgctgg
      481 atcatgatca cgagctcgtc gatctgggaa tcgttaagcg gaccgttgta ctcctggccg
      541 aaggccggca tcaggaccgt gccattggca gccttgagac cggtgtggac ccggttgcgg
      601 atgaaattgg cgcgcccttc taggccgctg ccgtactgag gattatttgg gtctgaccat
      661 ggcttgcccg cgttatcgcc ggtctgctgg atgccagttt gattgagcac gcggttcgac
      721 gtgtcaccgc cgatcggggc gccgatacgg ccggtacccg cctcgccggg cgcggtgtag
      781 ccctcaccgg ccggcccgtg gcaggcaaga cactgtgtca ggtacgtatt agtggcgcgc
      841 tcgacagcat cgtgctgctg ggcttccacc tgcgactcaa gtcgattcgg ctcatcggcc
      901 agatacagga tcaaaaccga cgccagtgcg actagcccta gcactacgac tgtggcgagt
      961 cgttgaactg cccccacgtt aatcgcactc ctacacgaat cggcgagccg aagacactaa
     1021 acgggcgtag cctgggacgg ctgataatcc tgccgcaggt tgatttcgcc ggtattgacc
     1081 agaaggtcgc cggttgacga atctacggtc gtagtcatgt agtccattgg acgcggtgcg
     1141 ggtccgccgg tacgcacgcc ctcgtagttg tagatcgagc cgtggcatgg gcagtggaat
     1201 gccttctcgg atggattcca gggcggaacc gtgcagccga ggtgcgggca gcgccagtac
     1261 aaggccatca cgccatcttg ggtatggacc aggtagaact tgccggcgga gtcgacatag
     1321 ggctgcccgt caacaggcgg tattagcgac gccggcaccc gcagggtctt cccgaacgcg
     1381 ccggtcttgt tcggccaaag gaatcggatc agtccgccgg tcagcagact gatattcaag
     1441 accactccac cgagcgcggc attgcggacg aagctgcgtc ggccgagctt cttcggcact
     1501 gcagccggat cctgccggtg ggcccgggtc agatgggcaa ctgccctggc tcccagcact
     1561 cccgccactg tgcggactac ggatgtcatc cttgcgctcc ttgggtcgtt tcgcaaaaca
     1621 ttggcgtcat cctagtgatg gagcggcagg ttccacggcc acgtcagatc ctggccatga
     1681 ccgcggaacg aggttccgaa aatcgccagt acccagtacg ccgtgaagaa gccggtgaac
     1741 agggcgatga tcatctcgcg cctggtcggt cggaagatca acttaacggc cgcgacaagc
     1801 atcgcgatcg agatgaccat cacgatcgtc gggtagacga tcttgcccat gaactccggg
     1861 aacgaaacgg ttggcttgaa aatgaagtgg atgatgccca caccggtgaa gtcatcgccg
     1921 atgtgctcat cggtccagtc gataatcttg ttgccgatgc cccagcctga ccgcttgtcg
     1981 ccgatgacct cgtcggcaac gatatagatc gtcagcacga ccgtggtcca gatcgccgag
     2041 aacccggcaa tcttgcgacc cttcgcggac gtgccgagga tcccgacacc gagcggcgag
     2101 cgatcgaaat aggggatagc cgccaggccg accagcgcca gggtcggcac gatcacaccg
     2161 gcgagtcccg catccatatg aagcagcagc tcctgcagat tcaggaagta ccacggcgcc
     2221 ttggatgggt tcggagtggc gttggtattg gcgtgttcgg tcagcggcgc actcacaaag
     2281 atcgagagca gcgtcagcag accgaggaag accgtggcgc ttaccgcctc gacgacgacg
     2341 agcgacggcc agaccatcac ctcgtcttct gcaaggtcgg tcggtcgggc cagggcccga
     2401 ccgcgcacca gctcaagcag cttttgccgc ttggcctcgt caatctgggc tgccgggccc
     2461 gcacccttgg gctcgtaggt aatctcagcc attcgtgctc acctcgatct ctgcgaccaa
     2521 atcgactaca gcgggccgga gataccgccg tccttgcgga tccgccagaa gtggacggcc
     2581 atcaggaagg cagcagccag cggcaggaag atgacgtgca gggtatagaa gcggatcagg
     2641 gtcgcctggc cgatctggta atcgccgacg agcatgaagt tgaccttcgg gccgaggatc
     2701 ggggccgcgc caccgatgtt cgtgccgacc gtgatcgccc agagcgaaag ctgatcccac
     2761 gggagcaggt agccggagaa ggagagcagg aacgtggtga tgagcaacaa gacgccgatc
     2821 acccagttga actctcgcgg cggcttgtac gagccggtga agaagacgcg ggccatgtgc
     2881 aggaagacgg cgatcaccat gccctgcgcc gcccagcggt gcatgttccg catcagcgtg
     2941 ccgaacgtga cgctcgagcc gaggtctttc atgttctcgt acgcgaggtt ggtggatggc
     3001 acgtaataga acatgaggat gatcccggtc atcaccagga ccaggaacat gaagaaggag
     3061 agtccgccga ggcagaaggt gtaggtcacc ttcgtggcat accgcttgat cttgaccggg
     3121 tggatgtgga ggaagacgtt gctggcaatg atcagcgcct ggttccgctg ggtgtccggg
     3181 tagccatgtc gcacaaccga ccgccacacc gacgaactcg taatgcgatc agcgagcgtt
     3241 tgctctcgcc tcatgcttgc tccctttcta accccggtcg gaaaccgcgt tttgtgacat
     3301 acgctgaccg acgtgttcgc gcattacctg tgcatacgat cagcgtctga attccgccgt
     3361 attctaccgc cgacttcgac aatccggtca acccgaatgc tgcatagctg cgcgcaaaaa
     3421 gaatcgccgc gatcgggtat cccggcggcg attttacgac tcgtcaaata cagcgtagat
     3481 gtgggcgcga caggactcga acctgtgacc tcacggatgt gaaccgtgcg ctctaaccaa
     3541 ctgagctacg cgcccgtgcg cggggcactg taccacagcg gtgaatccgc ttcccggcgc
     3601 cacgcgtaga ctccctcgat gcttcagcca acgacggcca cgcactcgac ctcgaccagc
     3661 accccccgcg gcaactttgc gacctgaacg gtgctgcgtg ccggtttgtg atcgccaaac
     3721 cagcgcccgt agatcgagtt gaacgcctgg aaatcgttga ggtcgaccag gaagcaggtg
     3781 gtcttgacga cccgggaaag ggaggagccg gcagcctgaa gaaccgcatc gagattcttc
     3841 atgacgcgct cggtctgcgt ctcaatatcc tcgccatcca cgtccatcga caccgggtcg
     3901 agcggaatct gacctgcggt gaagacaaga ttgccgatga cctgaccctg cgcgtagggg
     3961 ccaatggcgg ccggcgcgcc ttctgtcgcc acgatggaga gcgcgttggt tccctggtcg
     4021 gtcacagagt gcattccttt caaataacga tctattctcc gcggcgcgga tcagaggatg
     4081 cgcccgcgcc gcgccaccga ttagaataca ggctagcttc cccgcgcgcg ggcatctttc
     4141 aggagatata ggtggtagca gcagcacaga ctcggtatcg gcgaaccggc ctcgttcgtc
     4201 accacgaaca tgaacgaccc ctcgtccgat tgagccacac gctgaatgcc gtcgccggtc
     4261 ttggcgcgct tctcctcatt gcgaccgtca tcggattgtc gctctacgcc ctcggtcacc
     4321 agggtcgcat ctaccagggc gtcactgtcg ccggcgtgaa cgtttccggg atgaccgaga
     4381 ctgaagcggt cgaggcggtg cagcgcgact attccatata tatgaatacg ccgctgaacc
     4441 tgacgttcga gggaaagacc tacgcgatct cgccgaacga gctgggcatc cggctcgatg
     4501 cgcaggcgtc cgtcgatgcc gcgatgcagt atgggcgcga aggttctctt tggagccggt
     4561 cccgggcgtg ggccaagggg ttgctgacgg ggagcgacgt tccggccgtc ctgacggccg
     4621 acaattcccg ggtcgaccag gggctgttgg cattgaccga cgtggtcgct cgtcccgcca
     4681 cgaacgcgtg gatcgatttc tcgtccgatg aagcgaaagt ggtccctgag gttcccggcg
     4741 ttggctacaa ctacgggatg acccgtgccc tggtcatgaa ccgggtgcag acgcggagct
     4801 cagcgccgat tgagattgcc actaccgtcg tccagccggg cgtcacggcc cagacactgt
     4861 cttcgaccct gcccaccgcc cagacggccg tttcgagtgc gctcgtgttg caagggatca
     4921 atggccagaa ctggtcagtc gatgcacagc agctgaagtc gatcgtatcg gtcagctcgg
     4981 acggcacttc cgtcacggtc gaccgggatg cggtccagcg tttggtcagc ggcatcgccg
     5041 aatcggtgaa tcgggatgcg acggacgcag tcctcttcgt caacggggac ggcgaactgg
     5101 agatcgtgcc gggcgtcaac tcgctggccg tcgacgtcga caagacggtc gacgatgtcg
     5161 cctcgtcact tgtcgcagga tcgcacgacg tcgaagtcac gatagaacaa aagtcgcctg
     5221 cgattacaga cgagcgcgct gctgcctcgc tcgagcaaat cacgaagacg ctcgcgaccg
     5281 ggctgaccgt caagtgggac ggcggtcaaa agcagctgac cccgaccgat ttgctttcgt
     5341 cgctcgtgat caaggccact ccggatcagg ccgatgcatt cgcgttcagc ttgtcgcacc
     5401 aggtcctttc cgattacatc aagtccttcg ccggcgatat cgagatcgag ccgcacgagc
     5461 cgaccttccg gctggtgaac ggcgaggtca aggccgagaa aaagggccag accggcgtcg
     5521 taatcaatta cgactcctcg gccgagcgga tcgagaaagc cgtcttttcc ggctactcca
     5581 gctcgaacct gaaggtcgat gtcatcaaac cgaagttctc gaacaatgac gccggcgcga
     5641 tcaagctgcc agatgtcctg ggcgaggcag ccacgccata cagcaactcc agcgaggcgc
     5701 ggaagaccaa tgttgagcgc gcggtcgacc tgatgaatgg ctggctgatc gcaccgggtg
     5761 atgagttctc gtacgtggac catatcggtc aggtaaccga ggacaacggg ttcgaggtcg
     5821 gcctcgggat cgtcgccgat ccgaacaacc ccggcgcggt gatgactgcg ccggtcgtcg
     5881 gcggcggcat ctgccaggtc tcgaccacca tcttccagtc cgccttctgg gccggcctga
     5941 cctttaccga gcggcaccag cacccgtact ggatcaatag ctatggaatc ggcgagggcg
     6001 gcatgaaggg gctcgacgcg atggtcaaca tcgaggacga gccgaacgag tgggcgatca
     6061 ccctcgacat gaagttcgtc aacaccaccg atcattggat cgcgatcgag atgaccgccg
     6121 atggcgacaa cgtgacctcg cggatccttg gcaccaaccc aggatggacg atcgacgtca
     6181 gcgacccgga tatcacagcc atcaccaagc cggacccgac gccaatccgc caggactcac
     6241 ctgaagtccc gtccggcgag gagcggcaag tagagacagc ccaggacggc ttcgatgccg
     6301 acatccatcg cgtggttaag gacaagagcg ggactgttct cgatacctac gacgtgagca
     6361 gcagttactc cgcgacctcg aaccggattc tggtgggaac gggtcagtaa cgccgcacgt
     6421 tacgaaacgg aatcgactgc cagaacgaac ggcgtacgac cggaaacggg tggtacgccg
     6481 tttgtcattt cgttatactt ggtcgctgca gacgaccgat acggtctcag cggaaatgag
     6541 tcaggcaaat ctgccttgac gccccgctga acaataggta tcatctgctg gctctcgacg
     6601 agagagcatt cgctcgcgcc gaacggtgcg agccgcaatt cgcgtccatc gagacatgga
     6661 tgcgcgcaag ggagtgcggc cggggggcgg cgcggaagac ggagtggagc ctgggttcgg
     6721 gcggagcaat ccgggccgga tccagcaacc aacctgcacg aacaaagaac gaagacctgg
     6781 cggtcggggc ggggtgatgg
//
LOCUS       DATAOZ010000128         7034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2945574, whole genome shotgun sequence.
ACCESSION   DATAOZ010000128 DATAOZ010000000
VERSION     DATAOZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2945574"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VHR64_03590"
     CDS             <1..706
                     /locus_tag="VHR64_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301937.1"
                     /translation="TYASRGTESVQDVIVQQFPVSARIGLVAIIFAVLVGVPLGIIAS
                     LRQNTWVDYLSLLTSTIGISVPTFVSGLILLIFMSRQFHVSPIKRPEAWEGLFSTAYI
                     LPGIVLGLGTTAYITRLTRSSMLEVMKDDYVRTARAKGLRERVVVPRHMMRNALIPVI
                     TIIGPAAADLVTGSIIIETIFNAPGIGKEFVTAIGKRDYSVIMGLTVFYAVLIALANV
                     LVDLSYGFIDPRIRRR"
     gene            703..1737
                     /locus_tag="VHR64_03595"
     CDS             703..1737
                     /locus_tag="VHR64_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3301938.1"
                     /translation="MSSTELSEAVPAPAIGAPPASLHVAADSPLTAMRPQRSLWSNAW
                     RRLRKNVMAMVALFFLFFLLIVAIFAPVIAPHNPVKTDVAVAGTLRQAAWVDHPNPKK
                     DGTWTYPLGTDSVGRDVFSRLVYGTRVSLVVGFIPMVVILLIGVPVGLIAGFAGGRID
                     NLLMRITDVVYAFPALLLAIIMQISFGDTGFGKLLNGLVLLFLSLSIVNWTGVARLVR
                     GETLSLKEKEFVEAAKTSGASKFRLITRHILPNSLGPIIVAGAFIVPGAIISEAILSY
                     LGVGVRPDVNLNAPFPTSWGQMILDGSKTYNSQLWMLLAPSIAVALTTLSFTFLGDGL
                     RDALDPRQSD"
     gene            1862..4159
                     /locus_tag="VHR64_03600"
     CDS             1862..4159
                     /locus_tag="VHR64_03600"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX3301939.1"
                     /translation="MPGTSKATLITDQSASDQDSQTPNLRPPVSLPAEHLIEAARSRL
                     RTELAQHNLGDVPAIIERAVDRAIEWHAGQVRKSGEPYVVHPIEVAILLSRMSVDTEA
                     IIAALLHDVVEDSAISLDTVRQEFGDRVALLVDGVTKLGQIQWTGDGDQATRERAVQA
                     ENLRKMFLAMIDDIGVVLIKLADRLHNMRTLDHMPRDKQIQIAQQTMEIYAPLANRLG
                     IWQFKSELEDLAFRYLQPEAYTSIRISLEARGRDQASYIARVMQELKEAILAEGIDVE
                     LTGRTKHIYSVFRKMRQKQRTFDEIYDVIGIRCIIPNDHVKDCYGVLGVIHSMWHPIP
                     GEFDDYIATPKQSMYQSLHTAVIGPEGHALEIQIRTQRMHEIAEFGVAAHWRYKEGKK
                     ADAKVEAKVAWLRQMLEWQAEMKDAEDFVETVKSDVLEEMIYVFTPKGDIIELPAGAT
                     PLDFAYRIHTEVGHHTVRAKVNDQLVRLDTKLKNGQVVEVSTSKTKLGPSRDWLREDL
                     GYLTTASAKEKIRQWFRRQEREENLAQGKEILEKELRRLGVELKPEDIHKHFPRYNKV
                     EDMIAAIGYGAISPQLIASRLGENETKNVLSDTPHVPKPRVPLRLDVMGVGDLLTSLA
                     TCCKPVNGDAIVGYVTRGRGITVHRADCSNLVNLPDPERLIQVTWGSKAGETYPVNIM
                     VRAWDRVGLLKDISTLLADEKVNILYVQTVTNDDRTVSLRITIEVENVSQLSRVLHKI
                     ESVPAVDEVRRDTSNGSSATRLAGD"
     gene            4164..5108
                     /locus_tag="VHR64_03605"
     CDS             4164..5108
                     /locus_tag="VHR64_03605"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX3301940.1"
                     /translation="MTQTSTVLSLGEVLIDMIVSDGSPTLEDAGEFTARPGGAPANVA
                     VALRRLGVPSAFCGAVGDDPFGSRLSRVLLADNVDTGSLKVVPGASTTLAFAWKNERG
                     DGQFRLVRLADVLLSELDIEQAGIADRAAIIVGSVALTASPSREAIYRAVELASEAGV
                     PVCFDVNMRPSLWESGERAREICQPVLDRTTILKLSVDDADVLFGLRHPDEIFAARVG
                     SDAVRLLTDGGRGAWFRNESGEVEHVPAFPIDPVEPTGAGDAFTAAVVSRYLASQRAP
                     NRHDIAFASAAGAITATRIGAIESLPTRAEIEQFLSSR"
     gene            5152..>7034
                     /locus_tag="VHR64_03610"
     CDS             5152..>7034
                     /locus_tag="VHR64_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3301941.1"
                     /translation="MTQRIQPENQGTPGDDGTAQLMRQSADLRLLRRVREQLAASDDF
                     ESIIRAAVVALAGQAGYAYVSAFLTQPGGLALVHQVGYERPLRWLDIESGIRGRVARS
                     GVGELVSDVTADSDYVGTGLEIGSEICVPFGEETEAAGVLVVESPVPRTLWATDFRIV
                     SEIAGLLTLAIERAALAKAHRTSEQRLRLALDAAAMGIWTWTIAEGTIDWQFAVSDGA
                     DSQIRSIDDLLAHAHAGDFAHLAHAFGLAATDGDLDVEFRLVGNSGETRWLNLRGHAI
                     DRAADGTPRVIAGVASDITGRKRLEEERLRLVHLETARLNAEEAQRAMAETIERLRDG
                     FVAVDSELRLSLVNDEAARLFGGNRADLLGAPLQDALGSLGDEPAVANLVAACHSTEP
                     SSFDLLDAQAERFLEVRVFPGRDGSTLHLRDVTALRRAEYERHRIEARFRSLVQESSD
                     LILIMTRKAMIEFASPAVERMLGFRPEEIIGRRDSLTVHPADEKRFRHALIRILRTPG
                     TNPPFEVRVQHRDGSYRWLEVTPTNLLADPLIHGVVANCRDITERHASEFNLWLLSEI
                     STVVGSSLDLAQTLEAVNRLLVTYVCEVSLVAVFNERGDVERFAAAEREPYRPSFGDG
                     RQLA"
BASE COUNT         1306 a         2171 c         2243 g         1314 t
ORIGIN      
        1 gacctatgcg tcccggggaa cagaatcagt ccaggatgtg atcgtccagc aattcccggt
       61 ctcagcccgg atcggactgg tggcgatcat cttcgcggtg ctggtcggtg ttccgttggg
      121 gatcatcgca tcgctccggc agaacacctg ggtcgattac ctgagtttgt tgacctcaac
      181 gatcggcatt tcggtcccca ccttcgtgag cggcctgatt ctgctcattt ttatgagccg
      241 gcagttccat gtctcgccga tcaagcgccc ggaggcatgg gaagggctct tcagcacggc
      301 ttacatcctg ccgggcatcg tgctcggtct gggcacgacg gcgtacatca ctcgcctgac
      361 ccgatcgagc atgctcgagg tcatgaagga cgactatgtc cgtaccgccc gcgccaaggg
      421 cctgcgcgag cgtgtcgtgg taccgcggca catgatgcgg aacgcgctga tccccgtcat
      481 cacgatcatc ggcccggcag cggccgatct ggtgacgggc tcgatcatca tcgagacgat
      541 tttcaacgct cccggcatcg gcaaggagtt cgtgactgca atcggcaagc gagactattc
      601 cgtaatcatg ggactgaccg tcttctacgc tgtgctgatc gcgctcgcga acgtcctggt
      661 tgacctgagc tatgggttca ttgatccgcg gattcgacga cgatgagcag tactgaactt
      721 tccgaggccg tccctgcgcc cgcgattggt gccccaccag cgagcctgca tgttgcggcc
      781 gattcgccgc tgacggccat gcgtccgcag cgatcgctct ggagcaacgc ctggcgccgc
      841 ctgcgcaaga acgtgatggc gatggtggcg ctcttcttcc ttttcttcct gctgatcgta
      901 gcgatcttcg ccccagttat cgcgccgcat aacccggtca agaccgacgt cgccgttgcc
      961 ggcacattgc ggcaggcggc gtgggttgat cacccgaacc caaagaagga cggtacctgg
     1021 acatatccgc ttggcacgga ttccgtaggt cgcgacgtct tcagccgact ggtctacggt
     1081 acgagggtct cgctggtcgt cggcttcatt ccgatggtcg tgattcttct gatcggtgta
     1141 ccggtcgggt tgatcgcggg gtttgcgggc gggcgcatcg ataacctgct gatgcgaata
     1201 accgatgtgg tctatgcgtt tccggcgtta ttgctggcca ttatcatgca gatttccttc
     1261 ggcgataccg gattcggcaa gctcctgaac ggactcgtcc tgctcttcct cagcctatcg
     1321 atcgtgaact ggaccggcgt ggcgcgcctg gttcgtggcg agacactctc cctcaaggaa
     1381 aaggagttcg tcgaggcggc gaagacgtct ggtgcaagta aattccgcct gatcacgcgg
     1441 cacattttgc cgaactccct ggggccgatc atcgtggccg gcgcattcat tgtcccgggc
     1501 gcgatcatca gcgaagcgat cctgagctat ctcggcgtcg gcgtgcggcc ggatgtgaac
     1561 ctcaacgcac catttccgac gagctggggc cagatgatcc tcgacggctc caagacctac
     1621 aactcgcagc tctggatgct gctcgcgccc agtatcgcgg tggcactgac aacgctatca
     1681 ttcaccttcc tgggagacgg cctgcgcgat gcgctcgatc ctcggcagtc ggactagaca
     1741 cgggcaggac ctgcggtttt tcgcacagat tgactgaaat ttaggcaaaa cctacacttc
     1801 acgcatcgtc ggatcacccc gtcggggatt ggctgtcgtc cgacgggacc agggaaatca
     1861 aatgccgggc acgagcaagg ccacgctgat cactgaccaa tcggcgtcgg atcaggattc
     1921 gcagacaccg aatctccgac cgccggtcag tttgcctgcg gagcatctga tcgaagcggc
     1981 ccgatcccgg ctgcgcaccg agctcgccca acacaacctc ggcgacgtac cggccattat
     2041 cgagcgcgcg gtagaccgtg cgattgaatg gcacgccggg caagtgcgca aatcaggaga
     2101 gccgtacgtg gtccacccaa tagaggtggc cattctttta tcccgaatgt cggtcgacac
     2161 cgaggcgatc atcgcggcgc tgctgcacga cgtcgtcgag gacagcgcga tcagcctcga
     2221 taccgtgcgg caggagttcg gcgatcgcgt tgcactcctg gtcgacggcg tgaccaaact
     2281 cggtcagatc cagtggaccg gcgacggcga tcaggcgacc cgggagcgcg cggtgcaggc
     2341 agagaacctg cgcaagatgt tcctggccat gatcgacgac attggcgtgg tcctgatcaa
     2401 gctggccgac cgcctgcaca acatgcgtac ccttgatcac atgccacgcg acaagcagat
     2461 tcaaatcgcg cagcagacga tggagatcta tgcgccgctg gcaaaccggc tcggtatctg
     2521 gcaattcaag tcggagctgg aggatctcgc tttccgctac ctccagcccg aggcgtacac
     2581 ctcgatcagg atcagcctgg aggctcgcgg tcgggatcag gccagctaca tcgcccgggt
     2641 catgcaggag ctcaaggaag caatcctcgc tgagggtatc gatgtcgagc tgaccgggcg
     2701 gaccaagcac atctattcgg ttttcaggaa gatgcggcag aagcagcgga cattcgacga
     2761 gatctacgac gtcatcggta tccgctgcat cattccgaac gaccacgtca aggattgcta
     2821 cggcgttctc ggcgtaattc attcaatgtg gcacccgatt ccgggcgaat tcgacgacta
     2881 catcgcgacg cccaagcaaa gcatgtacca gagcttgcac accgccgtga tcgggccgga
     2941 gggccacgcg ctcgagatcc agattcgcac gcagcgcatg cacgagatcg ccgagttcgg
     3001 cgtcgccgcg cactggcgat acaaggaagg caaaaaggcc gacgccaagg tcgaggccaa
     3061 ggtggcgtgg ctccggcaga tgctggagtg gcaggccgag atgaaggacg ccgaggactt
     3121 cgtcgagacc gtcaagtccg atgtcctcga ggagatgatc tacgtcttca cgccaaaggg
     3181 cgacatcatc gagctcccgg cgggcgcgac cccactcgat ttcgcctatc gcatccacac
     3241 cgaggtcggt catcacaccg tccgggccaa ggtcaacgat cagctcgtgc ggctcgatac
     3301 caagctcaag aacgggcagg tcgtcgaagt ctcgacgagc aagaccaagc tcggcccgag
     3361 ccgggactgg ctgcgggagg atctgggcta cctgacgacc gcctcggcca aggagaagat
     3421 caggcagtgg ttccggcgac aggagcgtga ggagaacctg gcgcagggca aggaaatcct
     3481 cgaaaaggag ctccgccgcc tcggtgtgga gctcaagccg gaggacattc acaagcactt
     3541 cccgcgctac aacaaggtcg aggacatgat cgcggcgatc ggctacggcg cgatctcgcc
     3601 acagctgatc gcgtcccggc ttggagaaaa cgagaccaag aacgtcctct ccgatacccc
     3661 gcacgtgccg aagccgcggg ttccactgcg gctggacgtg atgggcgtcg gcgatctgct
     3721 gacgtcgctc gcaacgtgtt gtaagccggt aaacggcgat gcgatcgtcg gttacgttac
     3781 acgcggccgc ggcatcacgg tccaccgggc cgattgctcg aatctggtca acctgcccga
     3841 ccccgaacgc ctgattcagg tcacatgggg ctcgaaggcc ggcgaaacgt atccggtcaa
     3901 tatcatggtc cgcgcctggg accgggtcgg cttgctcaag gacatcagca cgctgctggc
     3961 ggacgagaag gtcaacatcc tctacgtcca gacggtgacc aacgacgacc ggaccgttag
     4021 cttgcggatc acgatcgagg tcgagaacgt gagccaactc agtcgcgtcc tccataagat
     4081 agagagcgtg ccggcggtag acgaagtcag gcgcgacacc agcaacggct cgtccgcgac
     4141 ccggctcgcc ggcgactgac gccgtgaccc agacctccac tgttctcagc ctgggcgaag
     4201 tgctgatcga catgatcgtg tcggacggtt cgccgacgct tgaggatgcc ggcgagttca
     4261 cggctcgtcc cggaggcgcg ccggccaatg tcgcggtcgc tcttcggcgc ctcggagtgc
     4321 cgagtgcatt ttgcggcgcg gtaggagatg atccgttcgg ttcgcgcttg agccgcgtgc
     4381 tccttgccga caatgtcgac accggcagcc tgaaagtggt gcccggcgcg agcacgaccc
     4441 tggcatttgc ctggaagaat gagcgcggcg acgggcagtt ccgactggtt cgcctcgccg
     4501 acgtcctctt gtccgagttg gacatcgaac aggcgggcat tgccgatcgt gccgcgatca
     4561 tcgtcggctc ggtcgcgctg accgcatcgc cgtcccgcga ggcgatctac cgggccgtcg
     4621 agctggcctc ggaagcaggt gtgccggtct gcttcgatgt caacatgcga ccgagcctct
     4681 gggagtccgg cgagcgagcc agggagatct gtcagccggt cctggaccgg accacgatcc
     4741 tgaagttgag cgtggatgac gcggatgtgc tcttcgggct gcgccatcca gacgagatct
     4801 tcgcggcgcg ggtcgggtcc gatgccgtcc gcctgctgac tgatggtggg cgaggcgcgt
     4861 ggttccggaa cgagagcggc gaggttgaac atgtgcccgc tttcccgatc gatccggtcg
     4921 agccgaccgg tgcgggcgat gccttcaccg cggcggtcgt gagccggtac ctggcttcgc
     4981 agcgggctcc gaaccggcac gacatcgcgt tcgcgtccgc ggcaggagcc ataacggcga
     5041 cgcgaattgg cgctatcgag tcattgccaa cccgggctga gatcgagcaa tttctgagca
     5101 gccggtagcc cggtttccgt tctgcatgct gacggatcgg gagagagggt gatgacccaa
     5161 cgaatccaac ccgagaatca aggtacgccg ggagacgacg gtactgccca actcatgcgg
     5221 cagtcggcag atctccgact gctccgccgt gtccgcgaac agcttgcggc gtccgatgat
     5281 tttgagtcca tcatccgagc tgccgtcgtg gccctggcgg ggcaggcggg gtatgcctat
     5341 gtcagcgctt ttctgacgca accgggcggg ctcgccctgg tccatcaggt cggctatgaa
     5401 cgtcccctgc ggtggctgga catcgaatcc ggtatccggg gtcgggtggc caggtccggc
     5461 gtcggcgagt tggtctccga cgtcactgcc gacagcgact acgttggaac cggtcttgag
     5521 atcggaagcg aaatctgcgt tccgttcggt gaagagactg aggcagccgg cgtcctggtc
     5581 gtcgaaagtc cagtgccgcg cacactttgg gccaccgatt tccggattgt cagcgagatc
     5641 gccggattgt tgacgctcgc aatcgagcgt gccgcccttg ccaaggcaca tcgcacgagc
     5701 gagcagcggc ttcgcctggc cctcgacgcc gcagcaatgg gcatctggac ctggacaatc
     5761 gctgagggga cgatcgattg gcaattcgcg gtgagcgatg gtgcggacag ccagattcgg
     5821 tcaatcgacg acttgttagc gcatgctcat gccggtgact tcgcgcatct cgctcacgcg
     5881 tttggactgg cggccaccga tggtgacctc gatgtcgaat tccggctcgt cgggaattcg
     5941 ggggagacac gctggctgaa cctgcgtggc cacgcgatcg accgagcagc cgatggcacg
     6001 ccccgcgtga ttgccggtgt cgcatccgac atcaccgggc gcaagcggct tgaagaagag
     6061 cggttgcgcc ttgtccacct cgagaccgcg cggctcaatg ccgaagaggc gcaacgcgcg
     6121 atggcggaga cgatcgagcg cctcagagat gggttcgttg ccgtcgactc cgagctgagg
     6181 ctatcgctgg tcaacgacga ggccgcccga ttgttcggtg gcaatcgcgc cgatctgctg
     6241 ggcgcgccat tgcaggatgc gttgggctcg ctcggcgatg agccggcggt cgccaacctc
     6301 gtcgcggcct gccactcgac ggagccgagc tcgttcgatc tcctcgacgc gcaggccgag
     6361 cggttccttg aggttcgcgt gtttccgggc cgggatggca gcaccttgca cctgcgcgac
     6421 gtgacggcgc tcaggcgggc cgaatacgag cggcaccgga tcgaggcgcg attccgctcg
     6481 ctggtgcagg agtcgtccga cctgatcctg attatgacac gcaaggcaat gatcgagttc
     6541 gccagcccgg ccgtcgagcg catgcttggc tttcgacccg aggaaatcat cggtcggcgc
     6601 gatagtctga cagttcaccc ggcggacgag aagcgattcc ggcacgcgct gatccgaatc
     6661 ctgcgaaccc cggggaccaa tccaccgttt gaggttcgcg tccagcaccg ggatggctcg
     6721 tatcgctggt tggaagtgac gccgacgaat ctgcttgcgg atccgctgat ccacggtgta
     6781 gtggcgaact gtcgcgatat caccgagcgg cacgcatccg aattcaatct gtggctgctc
     6841 tccgagatct cgaccgtcgt cggctcgtcg ctcgatcttg cccagacgct cgaagcggta
     6901 aaccggctcc tcgtcaccta cgtctgcgaa gtcagcctcg tggcagtctt caacgagcgc
     6961 ggcgatgtcg aacggttcgc agcggccgag cgcgagccgt atcgcccgtc cttcggcgat
     7021 ggacggcagc ttgc
//
LOCUS       DATAOZ010000129         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4524584, whole genome shotgun sequence.
ACCESSION   DATAOZ010000129 DATAOZ010000000
VERSION     DATAOZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4524584"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..1623)
                     /locus_tag="VHR64_03615"
     CDS             complement(331..1623)
                     /locus_tag="VHR64_03615"
                     /inference="COORDINATES: protein motif:HMM:NF020498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEX3301942.1"
                     /translation="MADLYIDYSRARPTVEKIKSLGCVGAVRYLSDRDLSDGITNPKD
                     ITASEAQTLLDGGLSLALVWETTASRAGAGFDAGVADAKAAEAQADALGYPGTAVIFY
                     VVDHDATVSAVMPYFDGIKTVAKRPIGVYGGYDIIHGLIHGLGFPYGWQTRAWSEGEV
                     CSRAHLYQRVFEPDWDLNDVLQPFPAWSKPEDPPPALVERIPVNTGRLAPIITGGSVT
                     TAYGVKGSTWAAGYHTGEDWNAPDDYGKPAYSAVEGKVVFAGTSGGWGPAYGKHVIVQ
                     DATSERTAHCHLCSIIVGLGENVVAGQELGLVGNTGNTTGAHVHVERRHRPFTYGSDE
                     KPARDLPRPANKVIKTVELVEGSFCDSVYWLAYALNKVKLEGGRNLRLSGRFDKLVGD
                     EVAKFQIQKCGDPGDRPIGPLQIKALLEHAGLTGFTVT"
BASE COUNT          326 a          596 c          576 g          353 t
ORIGIN      
        1 gggccagtcg agccgcccga gtagcgctcg atggcggacg gtgagaatcg ggggccgctg
       61 gcaagggctc cggaggctga tgtgtcgctc accacaggcc gaggagagtg cattgatact
      121 gcggcgtact ggcattgctg cactggtggt atccatcagc ctgggaatgg cgagcaatgt
      181 gttgttcttc gcggcgtttc agttccaatt cgatcggttc ttcgagccga cgctcatcgt
      241 tgcttcgggc gcgaccccgc tgaccccgac gaggtcgtaa cgaacgtggc cggagccgcg
      301 tccgagatct tcttcatcat gggagccgtc ctaggtgacg gtgaagccgg ttaggccggc
      361 gtgttcgaga agtgccttga tctgcagcgg gccgatcggc cgatcaccgg gatcgccgca
      421 cttttggatc tggaacttgg cgacttcgtc gcccacgagc ttgtcgaatc ggcccgagag
      481 ccgaaggtta cggccgccct cgagcttgac cttgttcaag gcataggcga gccagtagac
      541 cgagtcgcag aacgaaccct cgaccaactc aactgtcttg atcaccttgt tcgccggacg
      601 cgggagatcc cgagctggct tctcgtcgct gccgtacgtg aacgggcggt gacgccgctc
      661 gacgtggaca tgagctccgg tcgtattgcc cgtgttcccg accagcccaa gctcctgccc
      721 ggccacgacg ttctccccca ggccgacgat gatcgaacag aggtggcagt gtgccgtacg
      781 ctcgctcgtc gcgtcctgga cgatgacatg cttgccgtag gccggccccc agccaccgga
      841 tgtgccagcg aagacaacct tgccctcgac cgcggagtac gcgggctttc cgtaatcgtc
      901 gggagcgttc cagtcctcgc cggtgtgata cccggcggcc caggtcgagc ccttgacgcc
      961 gtacgccgtg gtcaccgagc cgccggtgat aattggagcc agcctgcccg tattcaccgg
     1021 aatccgctcg accagtgcag gcggagggtc ttcaggcttg ctccaggccg ggaacggctg
     1081 gagaacgtcg ttgagatccc agtccggctc gaagacgcgc tggtaaagat gcgccctgct
     1141 gcagacttcg ccctcggacc acgcccgcgt ctgccagccg tacgggaagc cgagaccgtg
     1201 gattagaccg tggatgatgt cgtatccgcc ataaacgccg ataggtcgct tagcaacggt
     1261 cttgatgccg tcgaagtacg gcatcaccgc cgagaccgtg gcgtcatggt cgaccacata
     1321 gaagatcact gcggtccccg ggtagcccag agcatcggcc tgcgcctcgg ctgccttcgc
     1381 gtcagcaaca ccggcatcga atcccgcccc agctcgcgaa gccgtggtct cccagaccaa
     1441 cgccaggctg agaccgccgt cgagcagggt ctgcgcttcc gatgctgtga tgtccttcgg
     1501 gttcgtgatg ccgtccgata agtccctatc agataggtat cggaccgcgc cgacacagcc
     1561 gagggacttg atcttctcca ccgttggccg tgcgcgcgag taatcgatat agaggtcggc
     1621 cataggttgt ccttacttcg tatgtgtgac cgcgagtcga accgtcgacc aatccatctg
     1681 accgtagctt gcccgttgcc aagctgatcg atcctcagac cctcgatttg gttggcacgc
     1741 accgcgcgac cgaaccacct cgtcatgttc tggtcatccg cgccgcccgc gatggctcga
     1801 gaaatgggcc acccggggat gacccgatcg accgcaagtg cgaccggcgc c
//
LOCUS       DATAOZ010000130         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4260712, whole genome shotgun sequence.
ACCESSION   DATAOZ010000130 DATAOZ010000000
VERSION     DATAOZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4260712"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VHR64_03620"
     CDS             <1..685
                     /locus_tag="VHR64_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX3301943.1"
                     /translation="SIWFENPNWLAAAIGLITAGLAFALQKVITSIAGYFVILRGDTF
                     SIGDRISMGGVRGDVIALGLIQTTIMEMGEPPSTLGGSSTWVRGRQYSGRIVTVSNAK
                     IFDDPVYNYTRDFPFIWEELSVQLPYSADRVLAEQILLDAAHRHTGEISERAHTAVTR
                     LGRQFPVQHATTEPRVYYALGTDRLELTLRFMTDARGERDVKDAISRDLLNAFDASGI
                     LLTPEAVAP"
     gene            complement(792..1271)
                     /locus_tag="VHR64_03625"
     CDS             complement(792..1271)
                     /locus_tag="VHR64_03625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3301944.1"
                     /translation="MHQTITLRPARVDEAEALSALTRRSKQSHGYSDEFMARFMSVGD
                     MVITPESISQNPVMVAEIDGRVAGFAHLKPLDHPDAVCLEDLFIEPDAQGQGVGRVLF
                     DWALATAAERGYDWLEWESDPNAAGFYEKMGGEKIGETKSTLIEGRVLPAYRMRVKS"
     gene            complement(1274..2158)
                     /locus_tag="VHR64_03630"
     CDS             complement(1274..2158)
                     /locus_tag="VHR64_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301945.1"
                     /translation="MTELVPVPSEHAGEYARLLIQIETRKQVIAGLRGERDGLEMTLT
                     NFAVELKGRVGALRVELNRVKRQVAEYRRRIERLRESDLLDPEQIEREVAEEFAERLE
                     QERIAEEQAAREGQRVDVERPRPQLDAETEAEILRLYRELAKRFHPDRARTESDRARR
                     TDLMLRINVAYSERDLVTLQAMAREADTGDPSTVLLSEEERVQWAHRVIARMNMQIAE
                     LTQQIEMLRRSETFQMWKSPEDSSESIQQVERRVRDRLNRERDRLDEAIIDYNRIVRR
                     RRMSHISVEREYASSGGE"
     gene            complement(2570..2953)
                     /locus_tag="VHR64_03635"
     CDS             complement(2570..2953)
                     /locus_tag="VHR64_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301946.1"
                     /translation="MLVGMRYVLLLLGVLTAFFMPRPLGAMGAQGQLIATIDKFAWLC
                     MIVLVLAAALVVLAMPQATMALFAAAGGTGLLFGIGRLDLVLAGSGAWMLFLAGLTWL
                     FRRIECWRRTRRQTTHLTRDLQPAS"
BASE COUNT          528 a         1042 c          978 g          568 t
ORIGIN      
        1 ctcgatctgg ttcgagaacc cgaactggct ggccgccgca atcgggctga tcaccgctgg
       61 gctcgccttt gccctgcaaa aggtcatcac ctcgatcgcc ggctacttcg tgatcctccg
      121 cggcgacacc ttcagcatcg gcgaccggat cagcatgggc ggcgttcgcg gcgacgtgat
      181 cgcgctcggc ctgatccaga ccacaatcat ggagatgggc gagccgccgt cgacactggg
      241 cggctcgtcg acctgggtgc gcggtcgcca gtacagcgga cggatcgtga cggtctccaa
      301 cgccaagatc ttcgacgacc cggtctacaa ctacacccgc gacttcccgt tcatttggga
      361 ggagctgagc gttcagctcc cgtactcggc cgaccgggtg ctggcggagc agattctgct
      421 cgacgcggcc caccggcaca ccggcgagat cagcgagcgc gcacacacgg ccgtgacccg
      481 cctcggccgc cagttcccgg ttcagcacgc gacgaccgag ccgcgggtct actatgcgct
      541 cgggaccgat cggctggagc tgacactgcg cttcatgacc gacgcccgcg gcgagcgcga
      601 cgtcaaggat gccatcagcc gcgacctcct gaacgccttc gacgccagcg gcatcttgct
      661 aaccccggag gcggttgcgc cgtgatctgt gatctttcgt ctctggtctt ttgtcttggg
      721 ttttgggttt tggtttttgg acggagcgta ccggctactg actaccggct acctttccgt
      781 tccctcaacc cttaactctt aacccgcatg cggtacgcgg ggaggacgcg gccttcgatg
      841 agagtcgatt tggtctcacc gatcttttcg ccgcccatct tctcgtagaa accggccgcg
      901 ttcgggtccg attcccactc cagccagtcg tagccgcgtt cggccgccgt ggccagcgcc
      961 cagtcgaaga gcacccgacc gacaccctgg ccctgcgcgt ccggctcgat gaagaggtcc
     1021 tcgagacaaa ccgcatccgg gtgatcgagt ggcttgaggt gcgcgaatcc ggccacccgg
     1081 ccgtcgatct cggccaccat gaccggattc tgcgaaatcg attcaggcgt aatcaccatg
     1141 tcaccgacgc tcatgaaccg ggccatgaac tcgtcgctgt agccgtggga ttgcttggag
     1201 cggcgggtca atgcactcag ggcctcggcc tcgtcgaccc gcgccgggcg gagagtgatc
     1261 gtctgatgca tgctcattcg cccccggagc tggcgtactc gcgctcgacc gagatgtgcg
     1321 acatccggcg gcgacggacg atccggttgt aatcgatgat cgcctcatcg agccggtccc
     1381 gctcgcgatt gagccggtcg cggacgcggc gctcgacctg ctggatgctt tcgctgctgt
     1441 cctcgggcga cttccacatc tggaaggtct cgctccggcg cagcatctcg atctgctggg
     1501 tcagctcagc gatctgcatg ttcatccggg cgatcacgcg gtgcgcccac tgcacccgct
     1561 cttcttccga gagcaggact gtcgaggggt cgccggtgtc ggcctcacgg gccatcgcct
     1621 gcaaggtgac gaggtcccgc tcgctatagg ccacgttaat ccgcagcatc aggtcggtgc
     1681 gacgggcgcg gtccgactcg gtgcgggcgc gatcggggtg aaaccgcttg gccagctcgc
     1741 ggtagaggcg caggatctcg gcctcggtct ccgcgtcgag ctgcgggcgt ggtcgctcga
     1801 catcgacccg ctggccctcg cgcgcggcct gctcctcggc tatccgctcc tgctcgagcc
     1861 gctctgcgaa ctcctcagcc acctcgcgct cgatctgctc gggatcgagc aggtcgctct
     1921 cgcgcaaccg ctcgatccgg cggcggtact ctgccacctg ccgcttgacc cggttcagct
     1981 cgacccggag cgcgccgacc cggcccttga gctccaccgc gaagttggtc agggtcatct
     2041 ccagcccatc gcgctcgccc cggagaccag cgatgacctg cttgcgcgtc tcgatctgga
     2101 tcaggaggcg ggcgtactcg ccggcgtgtt cgcttgggac agggacaagt tcagtcatgc
     2161 agtaacggcg cgcgaccgcc ggtcctcgcg ttggtgtcgg tttccgggcg cggccaagcc
     2221 tgatcgcaca cggcgtaaat aatcagcccc ctattgtccc acgtccgcgc ttcagaatcg
     2281 acgtttattg acgagttgtt cagggattga gcgtcggtca gtgttgagga ctgttgagtc
     2341 agcaggcggt ataaccgccg gaaccacagg ctagcttccc gccgcgcgtc caagcgataa
     2401 tcagccagag cccgagtgcc agcccaacga tgatcaggca tgccgactgc acccgtttcg
     2461 gccggtggtc attcactggg gcaatctcca tccgcccgct gggatgacgt gatgagtctg
     2521 cccaaccagg ggaaccacat gtttgcacgt cgtcaaccac aggacgagat caggatgcgg
     2581 gctgaagatc gcgagttaaa tgagtcgtct ggcgacgagt acggcgccaa cactcgatcc
     2641 ggcggaaaag ccaggtcagg ccggccagga acagcatcca ggcgccggag ccggccagga
     2701 caagatcgag ccggccgatc ccgaagagga gaccggtgcc gcctgctgcg gcgaagagcg
     2761 ccatcgtcgc ctggggcatg gccagtacga ccagcgccgc cgcgaggacg agcacgatca
     2821 tgcacagcca cgcgaacttg tcgatcgtgg cgatcaactg accctgcgca cccatcgccc
     2881 cgagcggcct cggcatgaag aacgcggtca atactccaag cagcagcaga acgtatcgca
     2941 tgccaaccag catgaccgcg ctcgccgcac ggtcaagctg actaggcggc gcaaaggtcc
     3001 cgttccaggt tattcagtgc tcggcgttgg catatccggc tcgtccggct ggagcttgga
     3061 ctcgacttcg gctcggagtt tggcgcgatc gatgcggaga gagtcgagga cgcgat
//
LOCUS       DATAOZ010000131         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_316239, whole genome shotgun sequence.
ACCESSION   DATAOZ010000131 DATAOZ010000000
VERSION     DATAOZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316239"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VHR64_03640"
     CDS             complement(<1..366)
                     /locus_tag="VHR64_03640"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX3301947.1"
                     /translation="MTFSLAGRCGRTGQFGVVVSSSSPAVAARCAHVAPGVGAACSQN
                     ITDPRLGPLLLRHVGQGLSARSAMSRVVRDAANIEFRQLTVIDTNGDTAFHNGSGVLG
                     THAAAQGTDAVAAGNLLRDE"
     gene            complement(363..776)
                     /locus_tag="VHR64_03645"
     CDS             complement(363..776)
                     /locus_tag="VHR64_03645"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3301948.1"
                     /translation="MAAHQRIRPFNTKDTYPEQKLDNDLCQAVRAGNIVFLRGQVGQD
                     LDTRESVGTGDAAAQTDKAMANIKMLLEEAGSELSHICKIVVYLTDVRYREPVYQTMG
                     KWLKGVFPCSTGVIVTGLARPEWVVEIDATAVIPG"
     gene            1003..1560
                     /locus_tag="VHR64_03650"
     CDS             1003..1560
                     /locus_tag="VHR64_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301949.1"
                     /translation="MNRRTVLSLAAATAAVQTLPRAANAQTSAFESGGLGLSIADWED
                     MLGPGEAGQTYMSFALDDGNFWVGVAGDSRAVDYIERNYADPNGVALADAQQEAVALI
                     PADARVLESFVANYAQILHGSQIDRYQSKALPAQFKGDTTRRYTRNFVIMYELAPAPA
                     AFDYFVTRYFVIAGTKPNGQDDSAT"
     gene            complement(1785..>2985)
                     /locus_tag="VHR64_03655"
     CDS             complement(1785..>2985)
                     /locus_tag="VHR64_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3301950.1"
                     /translation="RRAVDAVLDEVGGVVTRVGDETIGEMWSLYDGQDVLNEVNPQFS
                     ENITRHIARAIIEDPFHVSANTDPKGDRSKRPQDQDPDMLLHVVRETDRGIVVRGAKY
                     ETAAAYANQAFVKPTIANWGDAELSDYALGFIVQMGAKGLKHICRTGFAGKKPVEDYP
                     LTNRVDEVDTLVIFDDVEIPWEDVLFYRHTRAASYIRATLHRYSAFAFTQRVERLADL
                     MIGVALFNAKQTGLEKQQAVQEKLAELAGYREGINAHLTAAIAMAETSPGGLLMPNQS
                     LLYTGRVLASSQLPAMMHIARELCGGQIVVTPDFATFQHPETKPWMDKFYTINENWAA
                     EDRRRLLAFARDLLNSDYAGHRLTFQLFAQSPPFANLAAVYRSFDWNEALRVTKDAAG
                     LSDKVMN"
BASE COUNT          532 a          955 c          967 g          531 t
ORIGIN      
        1 ctcgtcgcgg agcaggttgc cggccgcgac cgcatcggtg ccctgcgcgg ccgcgtgggt
       61 gccgagcacg ccggagccgt tgtggaacgc ggtatcaccg ttggtgtcga tcaccgtcag
      121 ttgccggaac tctatgttgg cggcatcccg aactacccgg gacatggcgg accgggccga
      181 gagaccctga ccaacatgac ggagcaggag tggaccgagt cgcgggtcgg taatgttttg
      241 cgagcaggcc gcgccgactc cgggcgccac gtgcgcgcag cgagccgcca ctgccggact
      301 cgacgacgaa accacaacgc cgaattgacc ggtccgcccg caccggcccg ccagtgaaaa
      361 tgtcatcccg gaatcaccgc cgtcgcatcg atctcaacga cccactcggg ccgcgccaac
      421 ccggtcacga tcacccccgt cgagcagggg aagacaccct tcagccactt gcccatcgtc
      481 tggtagaccg gctcgcgata ccgcacgtcg gtcaggtaga cgacgatctt gcagatgtgc
      541 gaaagctcgc tcccggcctc ctcgagcagc atcttgatat tggccatggc cttgtcggtc
      601 tgcgccgccg catcgccggt tccgaccgat tcccgggtgt ccagatcctg gccgacctga
      661 ccgcgcagaa agacgatatt gccggcccgg accgcctggc agaggtcgtt atccagcttc
      721 tgctccgggt aggtgtcctt cgtgttgaac ggtcgaatcc gctgatgcgc cgccatgccg
      781 cctccaaatc tctcccctct cccgctgcgc aggagagggg tcgggggtga gggttaccac
      841 tctgccgaag gcaactcgcc cacattcaga gcctaaccaa gcaaaccgaa tagaacactg
      901 ggaagtattt cacgctttct ctcacccgac cgcgctacgg gatcggaacc aaaggttcga
      961 tatgatcgtt ggaatgccag gaaacgtacc caggaggaac caatgaatcg ccgaacggtc
     1021 ttgtcactcg ccgccgcgac cgccgcagtc cagaccttgc cccgcgctgc caatgcccag
     1081 accagcgcat tcgaatccgg cggactcggg ttgtcgatcg cggactggga ggacatgctc
     1141 ggaccgggcg aggcgggaca gacctacatg tccttcgctc ttgatgacgg gaatttctgg
     1201 gtcggcgtgg ccggggacag ccgcgcggtc gactacatcg aacggaacta cgctgatccg
     1261 aatggggtgg cgctggcaga tgcccagcag gaagcagtcg ccttgattcc ggccgatgcc
     1321 cgggtcctgg agagcttcgt cgcgaattac gcccaaatcc tccacggcag ccagatcgac
     1381 cgctaccaga gcaaggcgct cccggcgcaa ttcaagggcg acacgacccg caggtacacc
     1441 cgcaacttcg tgatcatgta cgagctcgcc ccggcgcccg cggcattcga ctatttcgtc
     1501 acccgctact tcgtcatcgc cggcaccaag ccgaatggcc aggacgattc agcgacgtag
     1561 cgcggcctga ccctgagctg cctggatggt gggttggacg ttaacgaagt tggtcggctc
     1621 catcgaccat ccatcctgtc accccaagcc ggccaccggg ggccggcact ccggttcgag
     1681 ctcaatgcat gcaccggctg cttcggagag aagacgccgc aatgccatgc gacgatagaa
     1741 ccgcgcgcct tcgcgcctcc cgacttcgcg ccttggttgc tcgactagtt catgacctta
     1801 tctgagaggc cggcagcgtc tttggtgact cgcagggctt cgttccagtc gaaactgcga
     1861 tagacggcgg cgaggttggc gaacggcggc gactgggcga agagttggaa cgtcaggcgg
     1921 tgaccggcgt aatcgctgtt gagcaggtca cgggcaaagg cgaggagcct gcggcggtcc
     1981 tcggcggccc agttctcgtt gatcgtatag aacttgtcca tccacggctt ggtctcggga
     2041 tgctggaagg tggcgaagtc cggcgtgacc acgatctggc cgccgcagag ctcgcgcgcg
     2101 atgtgcatca tggcgggaag ctggctggag gcgaggacgc ggccggtgta aagcaggctc
     2161 tgattcggca tcagcagacc gccggggctg gtctcggcca tggcgatcgc ggcggtcagg
     2221 tgggcattga tcccttcgcg gtaaccggcc agttcggcca gcttctcctg gaccgcctgc
     2281 tgcttctcga gaccggtctg cttcgcattg aagagggcga ccccgatcat caggtcggcc
     2341 aggcgctcga cccgctgggt gaaggcgaac gcgctgtagc ggtggagcgt ggcccggatg
     2401 tagctggcgg cacgggtgtg gcggtagaag aggacgtcct cccacggaat ctcgacgtcg
     2461 tcgaagatca cgagcgtgtc aacctcgtca acccggttgg tgagcgggta atcctcgacc
     2521 ggcttcttgc ctgcgaagcc ggtccggcag atgtgcttca gccccttggc gcccatctgg
     2581 acgatgaagc cgagcgcgta gtcggagagt tcggcatcgc cccagttcgc gatggttggc
     2641 ttgacaaagg cctggttggc gtaggccgcg gccgtctcgt acttggcgcc gcgcacgacg
     2701 atgccgcgat cggtctcccg gacgacatgg agcagcatgt ccggatcctg atcctgtggc
     2761 cgcttggagc gatcgccctt ggggtcggtg ttggccgaga cgtggaacgg gtcctcgatg
     2821 atggcgcggg cgatgtggcg agtaatgttc tccgagaact gcgggttgac ttcattgagg
     2881 acatcctggc cgtcgtacag cgaccacatt tcgccgatcg tctcgtcgcc gacgcgtgtg
     2941 acgacgccgc cgacctcgtc gagcacggca tcgaccgccc ggcgc
//
LOCUS       DATAOZ010000132         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1633364, whole genome shotgun sequence.
ACCESSION   DATAOZ010000132 DATAOZ010000000
VERSION     DATAOZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633364"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VHR64_03660"
     CDS             complement(<1..545)
                     /locus_tag="VHR64_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301951.1"
                     /translation="MVGIDEATDAAGPAGNSPSAFVIETRPLIRDWLCHWLSNVVCGL
                     KITPVMDPADLSRPLPDAVRLVVLSIGGASLSEAETLHVVANLVECLDKKPLLVVGDR
                     EEIDEIINAIQGGVRGYIPTSLDPLEAAEAVRFVLGGGTFLPAAVVVRSLRHRRDTSD
                     SCTTDRNGFTSLTRREMEVLA"
     gene            complement(604..1014)
                     /locus_tag="VHR64_03665"
     CDS             complement(604..1014)
                     /locus_tag="VHR64_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301952.1"
                     /translation="MSAVEAADSSITVLRCDERTLRREGLAGFLQLSGLRVWIPALDT
                     SQAAEPPADLAIIDTGERPRSHPGVNRSFEDLRSTLPSVPIVVISDREDGPAVIDAMQ
                     PGVRAYFPLSLDQKLLLETPVRRCRGRAELPPVT"
     gene            complement(1388..2650)
                     /locus_tag="VHR64_03670"
     CDS             complement(1388..2650)
                     /locus_tag="VHR64_03670"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX3301953.1"
                     /translation="MYIVGQEEIEALAAVIRKGALFRYGVGGECDRFERRYADFLGTR
                     HVALTASGTFALTAAVTALGIGPGDEVLVPAHTYMATAMAVLAAGAIPVIVDVDESIT
                     IDPEAVENGIGPRTKAVIPVHMWGVACDMDRIMAIAAKHDLLVLEDVCQAIGGGYESR
                     MLGTIGHAGAFSFNYYKNMTAGEGGAVCTADDRVALRVRCAVDPCHFYWQGRSDQQKP
                     FAGNGARASELMGAVLNVQLDRLPGMIEAMRAEKRQILDGTAQFGNLGLRPTPTNSPD
                     HDCAAQVMYSLPSAEAAERFAEVFPSVIAGKTGRHNYTEWDQILIGEGAAHPAMNPYN
                     MPQNRGCRRSYSRDMCQRSLDILNRTVMVATHPLHTPEQIADTIHNIGVAARVALGGL
                     SPAEADLRNVEPVDAQKFDLKVTDIAVA"
BASE COUNT          504 a          953 c          956 g          509 t
ORIGIN      
        1 tgggccaaga cttccatctc ccggcgcgtc aagcttgtga agccgttccg atcggtagtg
       61 cacgaatccg atgtgtcccg ccggtgtcgt agagagcgga caaccactgc tgcaggaaga
      121 aacgtacccc cgcccaggac gaaccgaacc gcctcggctg cctccagcgg atccagactg
      181 gtcggaatgt accctcgcac gcctccttgg atggcgttga tgatttcgtc aatctcctca
      241 cggtcgccaa cgaccaacaa tggtttctta tcgagacatt caaccaaatt agcgaccacg
      301 tgaagcgttt ccgcctcgga aaggctcgct ccgccgatac tcagcacgac cagccggacc
      361 gcgtccggga gaggtctcga cagatccgct gggtccatga caggagtgat cttgaggcca
      421 cagacgacat tcgaaagcca gtgacagagc caatcgcgga tcaggggacg agtttcgatc
      481 acgaaggccg atgggctgtt cccggccggt ccggcggcgt cagttgcctc atcaatgcca
      541 accattcgga cgctccctcc atcgcgcggc gtcggcataa gcgcgcctcg cgacggcttt
      601 gagttaggtc acggggggaa gctcggctcg acctctgcat cggcgaacgg gcgtctcaag
      661 gagtaacttc tgatcgaggc tcaacgggaa ataggcgcgc acgccaggct gcatggcgtc
      721 gatcaccgcc ggcccgtcct cccgatcgga aatcacgacg atcggcacgc tcggcaaggt
      781 gctccgcaga tcttcgaagc tcctgttgac gccgggatgg ctgcgaggcc tctcgccggt
      841 atcgatgatg gcgagatcgg ccggaggttc ggcggcctgg ctggtgtcga gcgcggggat
      901 ccacacgcgg agcccgctca gctggagaaa tccggcaagg ccctcccggc gcagtgttct
      961 ctcgtcgcac cgcagcacgg taattgatga gtccgcggcc tcgacggcgc tcatgcccgc
     1021 ccaccttcgt cagcagttca ggtcgctaaa ccctttttgg tctaacatgg agaagcgaag
     1081 gctacattca aggttgctgc atttgaatga taaatttccg gcaaatgcgt tgcagctccg
     1141 tcacaaaggg ccgccgaggt cccgacgccg tgagaagcgg gcgcagagca cggctaatcc
     1201 tttgatttac aggaattccg gaccgtaagc cgccaggtca gcgtgccctg acgtagcgcg
     1261 ggacctcgtc gggatgggag gcgatccgcc ttggaaacac cacttgcgga tcaagggcag
     1321 cccgcaggct gagctcgatg gcgtgcctgg cggtcccctc ggcgaccgag agctgggccg
     1381 gcttccgtca ggccacggcg atgtcggtaa ccttgaggtc gaacttctgg gcgtcgaccg
     1441 gttcgacgtt gcggagatcg gcctcggcgg gggacaggcc acccagcgcg acccgcgccg
     1501 cgacgccgat gttgtggatc gtatcggcga tctgctcggg cgtgtgcagc gggtgggtgg
     1561 ccaccatgac cgtgcggtta aggatgtcga gcgagcgctg gcacatgtcc ctggagtagc
     1621 tgcgccggca gccccggttc tggggcatgt tgtacgggtt catcgccggg tgggcggcgc
     1681 cctcaccgat caggatctgg tcccactcgg tgtagttgtg gcggccggtc ttgcccgcga
     1741 tcacgctcgg aaagacctcg gcgaagcgct ccgcggcctc ggccgacggc aggctgtaca
     1801 tgacctgggc ggcgcagtcg tgatcggggc tgttggtcgg cgtcggccgg agaccgagat
     1861 tgccgaattg cgcggtaccg tccaggatct gacgcttctc ggcgcgcatg gcctcgatca
     1921 tgccgggcag ccggtcgagc tggacgttga gcaccgcgcc catgagctca gaggcgcgcg
     1981 cgccgttgcc ggcgaacggc ttctgctggt cgctccggcc ttgccagtag aagtggcagg
     2041 ggtccaccgc gcatctgacc cgaagcgcga cccggtcgtc ggcggtgcag accgcgccac
     2101 cttcgccggc ggtcatgttc ttgtagtagt tgaagctgaa ggcgccggca tgaccgatcg
     2161 tgccgagcat gcggctttcg tagccgccgc cgatcgcctg acagacgtcc tcgagcacca
     2221 gcagatcgtg cttcgccgcg atcgccatga tccgatccat gtcgcaggcg acaccccaca
     2281 tgtggaccgg gatcaccgcc ttggtgcgcg gaccgatccc gttctcgacc gcctccggat
     2341 cgatcgtgat gctctcgtcc acatcgacga tgaccgggat cgcgcctgcg gcgagcaccg
     2401 ccatcgcggt cgccatgtag gtgtgcgcgg gcaccaggac ctcgtcgccc gggccgatcc
     2461 cgagcgcggt cacggccgcg gtcagcgcga acgtgccgct cgccgtcaac gcgacgtgcc
     2521 tggtcccgag gaaatcggcg tagcgccgct cgaaccggtc gcattcgccg ccgaccccgt
     2581 aacggaacag ggcacccttg cggatgaccg ctgcgagcgc ctcgatctcc tcctgtccga
     2641 cgatgtacat ggccgcttct ctgtccccag cagcccggaa ttctcgttcg gcgcggggcg
     2701 ggcttccggg cgacggcctt tccaggaacc aaggccccgc gcccggcacg atgtcataag
     2761 cgcgagctcc cggcaacgct cagcccgcgc acaccgacat ttgcgcttca gatcgacggc
     2821 cctggtacag ccgcggtcgt gctgatggtg cccggcgcag ataggaacgg cgcactttgc
     2881 tggcacgcaa gaggatgtgc gcgacagaga gatcgacccg gg
//
LOCUS       DATAOZ010000133         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1106927, whole genome shotgun sequence.
ACCESSION   DATAOZ010000133 DATAOZ010000000
VERSION     DATAOZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106927"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..184)
                     /locus_tag="VHR64_03675"
     CDS             complement(38..184)
                     /locus_tag="VHR64_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301954.1"
                     /translation="MLVLILVIRLTLALAVGGVGYSQYGCGGHSPAAIILIVLLVLLV
                     TGSL"
     gene            403..648
                     /gene="tatA"
                     /locus_tag="VHR64_03680"
     CDS             403..648
                     /gene="tatA"
                     /locus_tag="VHR64_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX3301955.1"
                     /translation="MIGDIGWQELAIVLVIVVIIFGAGRLPEIGGAVGKSIKEFRAQS
                     ADDKSPRTPIAPTTASADVAPSPARSSAAASIRADEV"
     gene            complement(856..1587)
                     /gene="tatC"
                     /locus_tag="VHR64_03685"
     CDS             complement(856..1587)
                     /gene="tatC"
                     /locus_tag="VHR64_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX3301956.1"
                     /translation="MTVGEHLLELRSRIVRACLSVGGAFIIGLILAVPMLNKMQHDSR
                     AVSGFDIRSPVDPITIYVKVAAYIALGLAFPAILHQLLAFVAPGLTRKEKRVVYSAIP
                     FVAILAILGGGYGFFVAAPRALYFLSHFMPHVFMWQPDAPEVITFYLTLMLGLALAFQ
                     IPVVMFILAKIGIVSPQRMAKVRKYAFIIILVIAAVITPSTDPFNMMVVAVPLYTLYE
                     FGIVTARLFARSTLITIPPPSQNAA"
     gene            complement(2071..2781)
                     /locus_tag="VHR64_03690"
     CDS             complement(2071..2781)
                     /locus_tag="VHR64_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3301957.1"
                     /translation="MANERVLIVDDEPQIRRALQTALAGHGYDVAVAADGDSAMILIG
                     AHPPDAIVLDLVMPGMDGFAVLREIRTWSEVPVIVLSARGQEQDKVSALDLGADDYLT
                     KPFGMAELLARVRVVLRRRATTHETVIVFGDVVIDLAARVVTKAGTEVHLTPREYDLL
                     RALAANSDKVLTHRHLLENVWGGYASENAQQLRVYINYLRRKLEDDPARPQWIVTEPG
                     VGYRLRTAVPLPGPEAEV"
     gene            complement(2771..>3439)
                     /locus_tag="VHR64_03695"
     CDS             complement(2771..>3439)
                     /locus_tag="VHR64_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3301958.1"
                     /translation="SHELRTPLTSITGFSEFLLKNGSRQTPEKLRYYCEILFLESSRL
                     SRMVDNLLDLTRIEGGTLHPDMSWYDIAELAADVANRPAARSSDHPLRLEISPDLPLV
                     CFDYVKIAQVLVNLIENAIKYTPAGTPVVVSARAAPHAVELAVTDAGPGIPADELDRV
                     FDSFYRSPRNARIPGTGIGLSIARGFVAAHGGRIWAERREGGGTSIRFTLPVETVDGE
                     RNGE"
BASE COUNT          654 a         1058 c         1141 g          586 t
ORIGIN      
        1 atttggttta ggaagccggg ttgctggcaa ttcgatgcta gaggctgccg gtcaccagga
       61 gtaccagcag gacgatgagg atgatcgctg cgggagagtg tccaccgcag ccatactggc
      121 tgtatccgac accgccaacg gcaagggcca gggtcagacg aatgaccaga atgagcacaa
      181 gcattgcgct ctcctgtcgc gacacggtcc cggactcttt aaggtcctga aaaatcatcg
      241 cgacatgagt atctctggca tcaggtgggg agtcgaccgc gcgctacagt ggcgaatccg
      301 cgatcggtcg agagccattt gggggcgcgc tgcgtatatt ccggcggttg caggaattcg
      361 gtttccggtc gatcccggcc gggacaggag cagtacggtc ccatgatcgg tgatattgga
      421 tggcaggaat tggccatcgt gctggtgatc gtcgtgatca tcttcggcgc cggccggctg
      481 ccggagatcg gcggcgcggt cggcaagagc atcaaggaat tccgcgccca gtcggcggac
      541 gacaagtcgc cccgcacacc tatcgcaccg acaacagcat cggcagacgt cgcgccgtcg
      601 cctgcaagga gctccgcggc agcgtcaatc cgggccgacg aggtctagga cgcaactgtc
      661 gctggtggct gttttgctaa cctgtgcact attctgtagc gtaatccaac actccagact
      721 gaaaaggtga tacaaaccgc aggacacggt ccccctagcc ccgtgtttct gtctgaccgg
      781 ggatgcccag ccaacatccc cgatcggacg ccctcagcaa tgtccatgcg tccccgcgct
      841 tccgggtaaa taccgtcagg cggcgttctg tgacggcggc gggatcgtga tcagggtcga
      901 tctcgcgaac agcctggccg tcacgatacc gaactcgtac agcgtgtaga gcggcaccgc
      961 cacgaccatc atgttgaacg ggtcggtgct cggggtaatc acggccgcga tcaccaggat
     1021 gatgatgaag gcgtacttgc gcaccttcgc catccgctgc gggctgacga tgccgatctt
     1081 ggcgaggatg aacatcacga ccgggatctg gaaggcgagc gcgagcccga gcatcagggt
     1141 caggtaaaac gtgatcacct cgggcgcatc cggttgccac atgaagacgt gcggcatgaa
     1201 gtggctgagg aagtacaggg cgcgcggcgc ggccacgaag aatccgtacc ccccgcccag
     1261 aatcgccaga atcgccacga acggaatcgc gctgtagacc acccgcttct ccttgcgggt
     1321 caaccccggc gcaacgaacg cgagcaactg gtggaggatc gccgggaagg ccaggccaag
     1381 cgcgatgtag gcagcgacct tcacgtagat cgtgatcggg tcgacgggcg agcgaatgtc
     1441 gaacccggac accgcccggg aatcgtgctg catcttgttg agcatcggca cggccaggat
     1501 caacccgatg atgaacgcgc cgccgaccga caagcacgcg cgcacgatgc gggagcgcag
     1561 ctcgagcaga tgttcgccca ccgtcatctc ggcgaacata tccggtgtgc cgtcatccgg
     1621 tcgcagccaa tcggggcggt gcggtcgatg cggccgggga ggatgcggcc ggtggagagc
     1681 gggtaatctg atggagtgcg acatctgtcc aactcggatc gggccggccg tcgatcgggc
     1741 tgcgcgtggc gcagctcggc cagtgcctac cgaaacaaac gtctcgggat tgccggcaga
     1801 tttcccaacc atgaagcgaa ccggtgttga cctgtcagag accgcaagat gcggccggcc
     1861 gtcgcgtacc ggacgggttt tccagggctc cgaaatgctc ttgacatccc ccttgacagt
     1921 gttcgtacac taggggaatc accaggcgtt gtatcccacg aattgagtag cggttggctg
     1981 gcgctacggg atggttggta ttttgtcgtg ttgtgacccg cctggtgaca tctcctgaga
     2041 agcccctgga aatcaagtga tgccacgtcg tcacacctcg gcttccggcc ccggcagtgg
     2101 tacggcggtg cgcaggcggt acccgacacc cggctcggta acgatccact gcggccgggc
     2161 cggatcatcc tccagcttgc ggcgcaggta gttgatgtag acccggagct gctgcgcatt
     2221 ctcgctcgca tacccacccc agacattttc gagcaggtgg cgatgcgtca ggaccttgtc
     2281 ggagttggcg gccagggcgc ggagcaggtc gtattcccgc ggggtcagat gcacctccgt
     2341 cccggccttg gtcacgaccc gggcggcaag atcgatcacg acgtcgccaa agacgatcac
     2401 ggtctcgtgc gtagttgcgc ggcgacgcag cacgacacgc acgcgggcca atagctcggc
     2461 catgccgaat ggcttggtca ggtagtcgtc ggcgccgaga tcaagcgcgg acaccttgtc
     2521 ctgctcctgc ccgcgcgccg agagcacgat gaccggcacc tccgaccagg tccggatctc
     2581 gcgcaacacg gcgaacccgt ccatgcccgg catgacgagg tcgagcacga ttgcgtcggg
     2641 cggatgggct ccgatcagga tcatggccga gtccccatcg gcggcgacgg cgacatcgta
     2701 gccatgcccg gcgagggcgg tttgcagggc cctccggatc tggggctcgt cgtcgacgat
     2761 caggacgcgc tcattcgcca ttgcgctctc cgtccacggt ctcgaccggc agcgtgaacc
     2821 ggatcgaggt cccgccgccc tcccgtctct ccgcccagat ccgtccgcca tgggccgcga
     2881 cgaatccccg cgcgatcgag aggccgatcc cggttccggg aatacgggca ttgcgcgggg
     2941 aacggtaaaa ggaatcgaag acgcggtcga gctcgtcggc cggaatgccg ggtcccgcgt
     3001 cggtcacggc cagctcgacc gcatgcggcg ccgcccgagc cgagacgacg accggggtgc
     3061 cggccggtgt gtacttgatg gcattctcga tcaggttgac caggacctgg gcgatcttga
     3121 cgtaatcaaa gcagaccagc ggcaggtccg gcgagatctc aaggcgcagt ggatggtcgg
     3181 acgagcgcgc cgccggccgg ttcgcgacat cggccgccag ctcggcgatg tcgtaccagg
     3241 acatgtctgg atggagcgtg ccgccctcga tgcgagtcag gtcgagcagg ttgtcgacca
     3301 tgcgcgacag gcgcgagctc tccaggaaga ggatctcgca gtaatagcgc agcttttccg
     3361 gggtctggcg cgagccgttc ttgagcagga actcgctgaa gccggtgatg gaggtcagcg
     3421 gggtgcgaag ctcgtggga
//
LOCUS       DATAOZ010000134         7897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2686431, whole genome shotgun sequence.
ACCESSION   DATAOZ010000134 DATAOZ010000000
VERSION     DATAOZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2686431"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VHR64_03700"
     CDS             <1..793
                     /locus_tag="VHR64_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3301959.1"
                     /translation="DSTLVVVDETAVFGNDFGPPSTLDLPGFKNEGKSFAPVQIAMTD
                     ADGKTKTNVTLIGIIDSSVSTLFGMYGSGPTLDPVIGPAPTERYYIQTTDSADTVAIA
                     RAIEGAVITDGVRVTSIQKALEDAQRISSGFLYMIQGFMGLGLIVGLAAVGVIAFRSV
                     VERRQQIGMLRAIGFQKQMVSFVFLLETAYVVILGIIAGTAMGLILARNLLTSDTEDL
                     NVEFHAPYPLIAIILIGTVLVALLMAWVPSRQASNIAPADALRYE"
     gene            complement(797..1966)
                     /gene="hemW"
                     /locus_tag="VHR64_03705"
     CDS             complement(797..1966)
                     /gene="hemW"
                     /locus_tag="VHR64_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX3301960.1"
                     /translation="MAPLPEHAPLGIYLHVPFCTHICPYCDFNTYAGQSDLIPRYVEA
                     MLRDLGTQSARIGRRPVTSAFFGGGTPSLLAADQVGRLVARAADLFDLRPDAEITLEA
                     NPNGLTREYLADLRAAGVNRLSIGLQTTDRRGLRRLGRMHEAEEAERAVEAAVDAGFD
                     RISLDLIFGWPGQEIESWRTDLKTVTRWAGGAVSHLSLYSLIVEPGTPLADAVSRGIV
                     TVPDDDAAADLYELAMDVLAGAGWLHYEVANWARSENQMSVHNGIYWRNGEYLGIGAG
                     AHGRVGHLRTMRHLLPATYIGAIEAGESPESNSEQLSPELQRGETMMLGLRLLREGVT
                     DAEFEQRHGIRLSDAFGPTIDRFQEQGLLDRDDRGVRLTRRGLMLANDVCAAFLD"
     gene            complement(2010..4811)
                     /locus_tag="VHR64_03710"
     CDS             complement(2010..4811)
                     /locus_tag="VHR64_03710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3301961.1"
                     /translation="MNPRQSPEADRTAVASGEVDQFSEPPRDGDSFTMPDWADQLVAI
                     GRSGFRATSRHEALIAIVAACVGIAGADQVLLQLAEPSPFDTAIVGSDGLSESDAPVI
                     LDAIENGDGDRVQQCDVALANGSARLLRVPAMEQDPAFVLTLIRFGETSYSASEAAIA
                     QLAADQVAMSLRQVTTESEIRQRTAERELIASLTESLVGMQDLAEVLTQVAAAVRTVS
                     GWDAVAVASYSWEWDWLSIDAIDQGVTQIPGVPQSGDRIWLADWHSLRFALDNCAPYQ
                     LSLELPEALTIHERAHMSEYGIGSILAIPFALGGEPTGVTLLYSQDGRALDAHGLGIV
                     QEVGANAALAIQHNQYMVQARQQAVEQAALLRVSKAVMSGQDLYAVLTEIARVSLGFY
                     GVEGCRIFLWHKDLDRFEIGAMQSVRDWATYYQISDTYPSADWPTCRVVLKDKVARGY
                     LITDPDLTARERANHVADQIQSLHRFPIVVGEEAVGILSFLSRSGRRFSPNTVRIGHE
                     LAAQAAHAVDRARLFGQLQRRAETDGLTGLLNHRAAFETLDRELAAARKLDEPLSIII
                     VDLDDFKLFNDTHGHLIGDKVLIEVAASLKESSRARDYVARYGGDEFLLILPGTNREQ
                     AAQVADRLLRRMASSQVLVGSLELPIRISVGVATFPHDAPNRQELIAYGDAAMYSAKE
                     CGGGQLGKIEKRTRSLEVTVFGALSGLVRAVDRKDRYTKDHSDLVAEYAVRLGRYLNL
                     GANELDALEVAGQLHDIGKIAVPDSVLRKPGQLTPEESAMIRQHVVFSEMIIQGIPNL
                     ELVHAAVAHHHERWDGMGYPHGKQGQEIPLLGRILTLADAMAAITMDRPYRKGRARDE
                     AIAELLRGAGTQFDPELVEQFVAAISTDTAILRDEDRRRRWIARDGGEVPADVVGLTD
                     YLRLRQEKHDSADDQRDSA"
     gene            complement(4959..5753)
                     /locus_tag="VHR64_03715"
     CDS             complement(4959..5753)
                     /locus_tag="VHR64_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3301962.1"
                     /translation="MGERVLSLEHVSVWLEDGSVILADISWSVQAGEHWAVLGPNGAG
                     KTTLFTVATARRYPSRGIVEVLGRRFGESSMLELREQISIVDPHQRMYDWFTVEEIVL
                     TGVTGTVQPQPDRYTDADARRARELIAQVGLAGMEEREIQSCSQGERQRVRVARALMT
                     RPRLLVLDEPASGLDLPAREALIASLTDLERSDPHLASMMVSHHLEELPPTITHALLL
                     REGRITACGPVNDVLTDEHVSEAFGIPVHVARHGGRWTARGAGTWT"
     gene            complement(5844..6260)
                     /locus_tag="VHR64_03720"
     CDS             complement(5844..6260)
                     /locus_tag="VHR64_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301963.1"
                     /translation="MRLSELGDKLKQMLGQAQTKASDATSKATDTASKAADQAKDISK
                     DLTDKAKSAADQAKTKADDMRSDASSAADSAKGSADSMKSEASAAADQASTAADSTQS
                     SMDDASAHVEAAKAHSDAAIADAKAAAQEAKDTLTQ"
     gene            6550..>7897
                     /locus_tag="VHR64_03725"
     CDS             6550..>7897
                     /locus_tag="VHR64_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3301964.1"
                     /translation="MFRCKVCGWIHDGIAPPNECPSCGATADRFRPMDAAEINLTTNE
                     LASYGIVGQGLDIDVDDTRRIRLLAFVDASFFSPYMERSGKRYPLYTGDAQWFSDNIP
                     CMSACPSHTDISRYIALIADGRYADSYELNREHNVFPGCLGRMCARPCEQACRRKEVD
                     APIGICYLKRVASDYRGETRREIPPPWNGLTAGIVGSGVNGLSAARQLARKGYKVTIF
                     EMQPVPGGVMWTGVPEWRLPRDVVMEEVEQILDLGIEVRYNVKIGRDISFRDLVDNFD
                     AVLISAGCQTAQGLGIPGEDLEGVVSGLQFLEDVNLGVKDVWVGKHCVTVGGGFTSMD
                     CVRSVMRMGSETSIMTYRRTIQEIPVEELELEEAEIEGVEIMYLITPIEVIGENGKVV
                     GLKCQKNQLGEPDKQGRRRPEPIPGSEFVIPCDMILVAIGQKQDSSFLGDLLPNRDR"
BASE COUNT         1377 a         2581 c         2506 g         1433 t
ORIGIN      
        1 ggactccaca ctcgtcgtgg tcgatgagac tgcggtcttc ggcaacgact tcggaccacc
       61 gtcgacgctc gacctgcccg gcttcaagaa cgagggtaag tccttcgccc cggttcagat
      121 cgcaatgact gacgccgacg gcaagaccaa aaccaacgtc acgctgatcg gcatcatcga
      181 ctcgtccgta tcgacgctct tcggcatgta cggctcaggt ccgaccctcg atccggtcat
      241 cggcccggcg ccgaccgagc ggtattacat ccagacgacc gacagcgcgg atacggtggc
      301 gatcgcgcgc gcaatcgagg gcgcggtgat cacggacggc gtgcgggtga cgtcgatcca
      361 gaaggcgctc gaggatgcgc agcgaatctc cagcggtttc ctgtacatga tccagggctt
      421 catgggcctc ggcctgatcg tcggtttggc ggccgtcggc gtgatcgcgt tccggtcggt
      481 cgtcgagcgc aggcagcaaa tcggcatgct ccgcgcgatc ggcttccaga agcagatggt
      541 ctcgttcgtc ttcctgttgg agacggctta cgtcgtgatc ctggggatca tcgccggcac
      601 ggccatgggt ctgatcctcg cgcggaacct gctgacatcg gacaccgagg atctgaatgt
      661 cgagttccac gctccatatc cgctgatcgc catcatcctg atcggcacgg tcctggttgc
      721 cttgttgatg gcatgggtgc cgtcgcgtca ggcctcgaac atcgcgccgg ccgacgccct
      781 gcggtacgag tagcggtcag tccaggaacg cggcacagac gtcgttcgcg agcatcaatc
      841 cacgacgcgt caggcggact cctcggtcgt cacgatcgag gagtccctgt tcctggaaac
      901 gatcgatggt cggcccgaac gcgtcggaca gcctgatccc gtgccgctgc tcgaactcgg
      961 cgtctgtcac gccctcgcgc aggagccgca gccctaacat catcgtctcg ccgcgctgca
     1021 actccggtga gagctgttcg gagttcgact ctggcgactc tccggcttcg atcgcgccga
     1081 tgtaggtcgc cggcagtagg tgtcgcatcg tccgcaggtg tccgacccgg ccgtgcgcgc
     1141 ccgcgccgat cccgagatat tcgccgttgc gccagtagat gccgttgtgg actgacatct
     1201 ggttctccga ccgcgcccag ttggcgacct cgtagtgcag ccagccggcg ccagcgagaa
     1261 cgtccattgc aagctcgtac agatcggccg cggcatcgtc gtcaggcacc gtcacgatcc
     1321 cgcgactgac cgcatcggcc agcggcgtgc ccggctcgac gatcaacgag tagagcgaga
     1381 ggtgtgagac cgcaccgcct gcccaccggg tgacggtttt gaggtcggta cgccacgact
     1441 cgatctcctg gcccggccag ccgaagatca ggtcgaggct gatccgatcg aatcccgcgt
     1501 cgaccgccgc ctccactgcc cgctcggctt cctctgcctc gtgcatcctg ccaagtcggc
     1561 gcaaaccgcg ccggtcggtc gtctggagtc cgatgctgag ccggttcacg cccgccgcgc
     1621 gcaaatctgc gaggtattcg cgggtcaatc cgttcgggtt tgcctcgagc gtgatttccg
     1681 cgtccggtcg caggtcaaac agatccgccg ctcgcgcaac caggcgaccg acctgatcgg
     1741 ccgccagcag ggacggcgtg ccgccgccga agaaggccga cgtgaccggt cgccggccga
     1801 tccgtgccga ttgcgtgccg aggtcccgga gcatggcctc gacgtagcga gggatcagat
     1861 ccgactgccc ggcataggtg ttgaagtcac agtacgggca gatgtgcgtg cagaagggga
     1921 cgtgcaagta gatgccgaga ggagcatgtt cgggaagcgg ggccacggtg agatcctgat
     1981 caatgcgacg cgttatgtcg ctgaccagtc taggcggaat cgcgctgatc gtccgctgag
     2041 tcgtgctttt cctgcctcag ccgcaggtag tcggtcaatc cgacgacgtc cgccgggacc
     2101 tcgccgccgt cgcgcgcgat ccatcgccgg cgccgatcct catcgcgcag gatcgccgta
     2161 tcggtcgaga tcgcggcgac gaattgctcg accagctcgg ggtcgaactg cgttcccgca
     2221 cctctcagaa gctcggcgat ggcctcatca cgtgcgcgcc ccttgcgata cggccggtcc
     2281 atggttatcg cggccatcgc atcggcaagt gtcaggatcc ggcccaggag cggaatctcc
     2341 tggccctgtt tgccatgtgg atagcccatc ccgtcccacc gctcgtgatg atgcgcaacc
     2401 gcggcgtgga ccagctcgag gttgggaatg ccctggatga tcatctcgct gaagacgacg
     2461 tgctggcgaa tcatcgccga ctcctcgggc gtgagctggc cgggcttgcg caggaccgaa
     2521 tcaggcacgg cgatcttgcc gatgtcgtgg agctgaccgg cgacctccag cgcgtcgagc
     2581 tcgttcgcgc cgagattcag gtaccggccg aggcgaacgg cgtactctgc aaccaggtcg
     2641 gagtgatcct tggtgtagcg gtccttacgg tcgaccgcgc ggaccagacc gctcagggcc
     2701 ccgaaaacgg ttacctccag cgagcgcgtc cgcttctcga tctttccgag ctggccaccg
     2761 ccgcactcct tggccgagta catcgccgca tccccgtagg cgatcaactc ctgccggttc
     2821 ggtgcgtcgt gcgggaaggt cgcgacaccg acggagatcc ggattggcag ctcgagactg
     2881 cccaccagga cctgggagct ggccatccgt cgcaggagcc gatcggccac ctgggcggcc
     2941 tgttcccggt tcgtgcccgg caggatcagc aggaattcgt cgccaccgta tcgcgccacg
     3001 taatcgcgcg cgcgggacga ttccttcagc gatgccgcaa cctcgatcag caccttgtcg
     3061 ccgatcagat gcccgtgcgt gtcgttgaac agcttgaagt cgtcgaggtc gacgatgatg
     3121 attgagagcg gctcatcgag cttccgcgcc gcagccagct cgcgatccag cgtctcgaac
     3181 gccgcccggt gattgagcag accggtcagg ccatcagtct cggcccggcg ctgcaattgg
     3241 ccgaacaatc gcgcccgatc aaccgcgtga gcagcctgcg ccgccagctc gtgaccgatc
     3301 cgcacagtgt tggggctgaa tcgtcgcccg gaccggctca ggaacgacaa gataccaacc
     3361 gcctcctcgc cgaccacgat cgggaagcgg tgcaggctct gaatctggtc ggcaacgtga
     3421 ttggcccgct cccgggcggt cagatctgga tcggtgatca ggtacccgcg cgcaaccttg
     3481 tccttgagga cgacccggca cgtgggccag tcggccgaag gataggtgtc cgaaatctgg
     3541 tagtacgtcg cccagtcgcg gacgctctgc atcgcgccga tctcgaatcg gtccaggtcc
     3601 ttgtgccaga ggaaaatccg gcagccctcc accccgtaga acccgagact gacgcgggcg
     3661 atctcggtca gcacggcgta gagatcctgc cccgacatga ccgcctttga aacccggagg
     3721 agggccgcct gttcgaccgc ctgctgccgc gcctgcacca tgtactggtt gtgctggatc
     3781 gccagggccg cgttggcgcc gacttcctgg acgataccga gaccatgggc atcaagtgct
     3841 cgtccatcct gcgagtagag cagcgtaacg cccgtcggct cgccgccaag cgcgaatggg
     3901 attgccagga tcgagccgat accgtactcc gacatgtggg cgcgctcgtg gatggtcagc
     3961 gcttccggaa gctcgagcga gagctggtac ggcgcgcagt tgtccagggc aaagcgcagg
     4021 ctgtgccaat cggcgagcca gatgcgatcg ccggattgag gcacccccgg aatctgggtg
     4081 actccttgat caatggcatc gatcgagagc caatcccact cccaggagta cgaggccact
     4141 gcaacggcgt cccagccgct gaccgtccgg accgccgccg caacctgcgt aaggacctcc
     4201 gcgagatcct gcatgccgac gagcgactcg gtcaggctcg cgatcaactc acgttctgcg
     4261 gtccgttgac ggatttcgct ttcagttgtg acctgccgca gactcatagc gacctggtcc
     4321 gcggcaagtt gggcaattgc cgcctcggac gcactgtagg acgtctctcc aaatcggatc
     4381 agggtcaaga caaaagccgg atcctgctcc atcgccggca cccggaggag cctggctgag
     4441 ccgtttgcaa gcgcgacgtc gcactgctgg acgcgatccc catccccgtt ttcgatcgcg
     4501 tccaggatga ccggcgcatc ggactcggac agtccatcgg acccgacgat cgctgtgtcg
     4561 aacggggacg gctcggcgag ctgcagcaac acctgatcag ccccggcaat cccgacgcag
     4621 gcggcgacaa tcgcgatgag cgcctcatgg cgggacgttg cccggaagcc ggatcgcccg
     4681 attgcgacca gttggtcggc ccaatcgggc atcgtgaagg aatcaccgtc ccggggcggt
     4741 tctgaaaact gatctacctc gccggacgcg accgccgtac ggtctgcctc cggcgattga
     4801 cgcgggttca ctcattcgtc cttgcgtggg tcgcaggcaa tcttgagttc atattaggaa
     4861 actgagatga ccgatcccat gggagcattt cgccagagtt tccggtacca acgtcacccg
     4921 ctcagggacg attcgccgaa cctggcggga cagccaaatc aggtccaggt gccggcgccg
     4981 cgagcggtcc agcgaccgcc gtggcgggca acgtgcaccg gaatcccgaa tgcttcggag
     5041 acgtgctcat cagtcaggac gtcgttgacc ggcccacagg cagtgatgcg cccctcccgc
     5101 agcaggaggg catgcgtgat cgtcgggggc aactcctcga ggtgatgcga gaccatcatg
     5161 ctcgccaggt gaggatcgga ccgctcgagg tcggtcagcg acgcgatcag cgcctctcgc
     5221 gccggcaggt ccaatccgct tgccggctcg tcgaggacca gtagccgcgg ccgcgtcatc
     5281 aacgcgcggg caacgcgaac gcgctggcgc tcgccctggg agcaggattg gatctcccgc
     5341 tcctccatcc cggccagccc gacttgcgcg atcaattccc gggcccggcg cgcgtcggca
     5401 tcggtgtacc gatcgggttg gggctgcacc gtgccggtga cgccggtcag gacgatctct
     5461 tcgaccgtga accagtcata catgcgctgg tgcgggtcga cgatgctgat ctgctcgcgc
     5521 agctcgagca tgctcgattc gccgaagcgc cggccgagca cttcgacgat gccgcgcgac
     5581 gggtaacgcc gcgcggtcgc gacagtgaag agcgtggtct tcccggcgcc gttcggacca
     5641 agtaccgccc agtgctcgcc cgcttggacc gaccacgaga tgtcggcgag gatgaccgag
     5701 ccgtcctcca gccacaccga aacgtgctcc agcgaaagaa cgcgttcacc caccgacggg
     5761 ccgtctccaa tatgcggaaa tgacagttgg acggcaaatg ccgtccaacc gactcgacgt
     5821 caggattgca ttatcgcagg gcgctactgg gtgagcgtgt ccttggcttc ctgggcggct
     5881 gccttggcat ctgcgatggc ggcgtcgctg tgcgcctttg cggcttcgac atgagccgag
     5941 gcgtcatcca tcgacgattg ggtcgagtcc gcggcggtgc tcgcctggtc ggcggcggcc
     6001 gaggcttcgc tcttcatgga atcggccgat cctttggcgc tgtctgcggc cgaggaggcg
     6061 tcgctccgca tgtcgtccgc cttggttttg gcctggtcag ccgccgactt ggccttgtcg
     6121 gtcaggtcct tgctgatgtc cttggcctga tctgccgcct tgctggccgt atcggtcgcc
     6181 ttgctcgttg cgtccgatgc cttggtctga gcttgcccaa gcatctgctt gagcttgtcg
     6241 ccgagttcac tgagtcgcat cgtacttccc cctggctgaa ccggattccg tacaccacgc
     6301 tgacccaaaa atcgagttgg cgcgggcatc catgccaaga ataccgcgaa atgcacaaga
     6361 atgaatcgaa tcggccaaac acttggtcaa tcgtacgtgt gtcggggata tcgaagtaat
     6421 cattcccaac cagcagccgg cggtgtttcg gttactcatc cggcggggct atacttgcaa
     6481 atgcgctggc ggcgttcgtc gctatgcggg tttgccattt tggcttcccc gggggagcat
     6541 ggaaatacga tgtttcgatg caaggtttgc ggctggatcc atgacggcat cgcgccgccg
     6601 aacgagtgcc catcctgcgg cgccacggcg gatcggttcc gacccatgga cgccgccgag
     6661 atcaatctga ccaccaacga gctggccagc tacggcatcg tcgggcaggg cctcgatatc
     6721 gacgtcgacg atacccggcg aattcggttg ctcgcattcg tcgacgcctc attcttctcg
     6781 ccctacatgg agcgctccgg caagcggtat ccgctctaca ccggcgacgc ccagtggttc
     6841 tccgacaaca tcccgtgcat gtcggcttgc ccgtcgcaca cggacatctc gcgctatatc
     6901 gcgctgattg ccgacggccg gtatgcggac agctacgagc tcaaccggga gcacaacgtt
     6961 ttcccgggct gcctcggacg gatgtgcgcc cgtccctgcg agcaggcctg ccggcgcaag
     7021 gaagtcgatg cgccgatcgg catctgctac ctgaagcggg tcgcgtccga ttaccggggt
     7081 gagacgcgcc gggagattcc gccaccgtgg aacggcctga ccgccggtat cgtcggcagc
     7141 ggggtcaacg gcttgagcgc ggcccggcag ctcgcccgca agggctacaa ggtcacgatc
     7201 ttcgagatgc agccggttcc gggcggcgtg atgtggacag gcgtgcccga atggcgcctg
     7261 ccgcgcgacg tcgtcatgga agaggtcgag cagattctcg acctcggcat cgaggttcgc
     7321 tacaacgtca agattggacg ggacatctcg ttcagggacc tggttgacaa cttcgacgcc
     7381 gtcctcatct cggccggttg ccagacagcg cagggcctcg gtatccccgg agaagacctc
     7441 gagggtgtcg tctcaggtct ccagttcctc gaagacgtca acctcggcgt caaggacgtc
     7501 tgggtcggca agcactgcgt caccgtcggc ggtggcttca cctcgatgga ctgcgttcgt
     7561 agcgtgatgc ggatgggttc cgagacgtcg atcatgacct atcgccggac gatccaggag
     7621 atcccggtcg aggagctcga actcgaagag gccgagatcg aaggtgtcga gatcatgtac
     7681 ctgatcacgc cgatcgaagt catcggcgag aacggcaagg tcgtcggtct caagtgccag
     7741 aagaatcagc tcggcgagcc ggacaaacag ggtcgtcgcc gtcctgagcc gatcccgggc
     7801 tccgagttcg tcatcccgtg cgacatgatc ctggtagcta tcggccagaa acaggacagc
     7861 agcttcctcg gcgaccttct cccgaaccgg gaccgcc
//
LOCUS       DATAOZ010000135         7231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4528109, whole genome shotgun sequence.
ACCESSION   DATAOZ010000135 DATAOZ010000000
VERSION     DATAOZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4528109"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..974
                     /locus_tag="VHR64_03730"
     CDS             129..974
                     /locus_tag="VHR64_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301965.1"
                     /translation="MTSTPTTSSDRSPFATTSSVPPYAEQVAEPPRTPESAEESGFAT
                     WNAPAPQPTETPGRSWDAPPPQNTAVPVDEDMIAALVGEAAPDEQEDVPAESAGVTAE
                     PTPEAPDPATWATPQPAAQVGQNGADSVDQLLRLVRELEYGLVELAESPASTVGSTSE
                     APAGDTRMLANALSDLQDEDDLMPLRNAVASAQDRPRDVDVMLDLVLRADAIAAVITE
                     RDQLKAAIELAVGGKPGADSQPTDDTDNRTVAQDEVVAESTSDDVAADTMEPAETADS
                     ESQSI"
     gene            1108..2301
                     /locus_tag="VHR64_03735"
     CDS             1108..2301
                     /locus_tag="VHR64_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301966.1"
                     /translation="MSADAAPAETTQKTLRIGSFIAARESEQGARTSPIPVIGAEETA
                     FAGKPGLAHWQQIGPTPDAIAEAEVKLAKLRHLSHPALAPVIESGLNKTVAWWIEAVP
                     PGRMLADAVRDDASLSSIPSVRRLVEVVSGVLALTHEVGLSHGSISGKSIVIGSNGAP
                     VLTGLGIGGKGFTQDQADLALLVINLLAGEPWNEQAADPLRRTDQLREHLGNYTDRIS
                     SVLARATHPDPAERYSLIADFAKQFGEAVQFSGEDLVQAGQDAISSQNAELAKLLAEK
                     AAAYDPNSDALANLNHRLRGGLPFATNGAQAMSNLDSPIPALPGTLSGLTPVDPAANM
                     RNQLPPELTEGLPQELIDLIAPQFEVKPVKKGVPPLLVMIVGFVGIAFLLALARMVTL
                     LITGN"
     gene            complement(2298..3239)
                     /locus_tag="VHR64_03740"
     CDS             complement(2298..3239)
                     /locus_tag="VHR64_03740"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX3301967.1"
                     /translation="MIDRPYVTVVGSVHTDLIASAVTMPTPGSSVIGNRFTLAPGGKA
                     GNQAAQLARLGVPTVLISRIGRDPLGNVIAGQLAASGVDLSFLARPEDAVTGASTVFA
                     VGGEYASIIVPGAAAGLTDTHLAAAEVVFRRSRFVLAQLELGTKLAIAAFIRARDAGA
                     TTVMNASPIQDVDPDALAEVLDLTDVLVVNRHEAAVLIAAPISDRADATLVSARLNER
                     FGLGATVITCGAEGMVLARGSQVIEQAAWPIEVVDTVGAGDAFLGALIAAWQRGDDDR
                     ASLRYGAAAGALAASAAGAFTSLPNRAEVYDFLRSRG"
     gene            complement(3236..4186)
                     /locus_tag="VHR64_03745"
     CDS             complement(3236..4186)
                     /locus_tag="VHR64_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3301968.1"
                     /translation="MPFQFTRFASRLNSFRVVSPARSPLDALRLLSTVPGITATELNY
                     PQHFSGPEDRTLITAAHSMGLAVTALNLRFDPPRFAAGSFTNPDPEVREAAIALAQQA
                     VDIAADRDVDHVILWLGPDGFDYPFQAEYGQLWQWAVDSVRAVAARHDAITVSIEPKP
                     SDPRRFSLVRGISDALLLVRDTGLGNVGVTLDFCHSLMAGESPAMAAGLAIRENRLIG
                     IHLNDGYGHLDDGLMVGSVHPVQTVELLLVLKRAGWNRTIYFDTFPEHLDPVAECAAN
                     IATVQRMVERLDSIDDGLLADIQAAQDAVSAMRLIQGLMP"
     gene            4402..6096
                     /locus_tag="VHR64_03750"
     CDS             4402..6096
                     /locus_tag="VHR64_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3301969.1"
                     /translation="MVSNEKDRILGVKAPRRTVLKGATGAAGLAAAGMIGKSAPQALA
                     QDDVKSQILAIPGAGKGQPTEQDMQKVGELCMGPTKANVQQGEFSGVELTFMGLNNQN
                     LHNIVFRNLLTSFVDYTGVKINWIDLAQADYNPRLQQAIATKTIDFDVVEMGAPYEGD
                     VCGAGLASEMPDWVKEQIDMSDYVGYLQPPVGTWNGKTYRVSIDGDCHNFNYRTDYFS
                     NADLASAWTSEGNQGEWAVPKTYQQVQAVTKFLKGKQIDGQDAYGWLDVCKPGGGFSW
                     YFFASRATAYAKHPDDKAWLFDADTMKPRINNPAFVRAIQDVIDALPSEPADQINADL
                     NVTGFSEFLGGTGSMCAWWGDVGSNVYTNDQSVVQDKVAFDILPGSDDVYNSKTGQWD
                     TLSSGPNYAPNMAYLGWGVYVMARVDTDPVKQKAAWSVAAFLGGKDVSLWLAAYPSGF
                     QPYRNSHFNVADWVATGYPEEFAKSYLASEQNSYNHPNAAIEPRIPGIFQYYSAAEDE
                     LAKAFAGQVDAQTCADNIAAAWEKITDDIGRDSQLALYQSSLGMSGGATPVASPSS"
     gene            6218..>7231
                     /locus_tag="VHR64_03755"
     CDS             6218..>7231
                     /locus_tag="VHR64_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3301970.1"
                     /translation="MQDSPAVSTSAWDAGSAAVPSSRQRTGQALISAATVGLLAIVSI
                     QTLHTVNVLDAGFANWRPVLYAYVAWALALCAGLVLSRGEHGRRAIFVLPAVLFTVSM
                     VIFPTVFGLYIAFTDWNLSSRSGRHFSGLDNFRQLIHDSYYRNSLINMIYYVLSVFVQ
                     YAIAFGLALLLNADVRGRKFFRVTFLLPFMLSPVAVSWMIGKSMMDLRFGPVSDLARR
                     LGWDTPAFFGSAWPARLSIMVMDAWVSIPFMMVLLLAGLQALPGEVLESAKVDGATGW
                     KSFWQMTFPLLLPVSITAVVLRIIFELKLADIVITMTGGGPGGATDTVTSFIYREYRD
                     RSNV"
BASE COUNT         1376 a         2340 c         2245 g         1270 t
ORIGIN      
        1 tggcggcgtt acgtgctcgc cggttcgtac gttggatgag gccaaagccc atgaatcgct
       61 gtccaaattg cgccgcccag aaccgcgaag gtgcgaagtt ctgcacaagt tgcggttttc
      121 gccttccagt gacatccacg cccacgacct catccgaccg ctcaccattt gcgacgacgt
      181 catcggtccc tccctacgcc gagcaggtcg ctgagccgcc caggacgccg gaatccgcgg
      241 aagagtccgg attcgcgacc tggaacgctc ccgctccgca acccaccgag acgcccggcc
      301 gctcgtggga tgcgccgccg ccccaaaata cggctgttcc ggtcgacgaa gacatgatcg
      361 ccgcgctcgt cggagaggct gcgccggacg agcaggaaga cgtgccagcg gaatcggcag
      421 gcgtgacagc ggagccgact cccgaagcgc cggatccggc gacgtgggca actccgcaac
      481 ccgccgctca agtcgggcag aatggcgcgg actccgtcga ccagctgctc aggctcgtac
      541 gtgagctcga atacggcctc gtcgagcttg ccgagtcgcc agcgtccact gttggatcca
      601 caagcgaggc gccggccggg gacactcgga tgcttgccaa tgcgctctcg gacctccagg
      661 acgaagatga tctgatgccg ctccggaatg ccgtcgccag cgcgcaggat cgcccgcgcg
      721 acgtcgacgt catgctcgac ctggtcttgc gggccgatgc aattgcggcc gtgatcacgg
      781 agcgcgatca gctcaaggct gccatcgagc tcgcggtcgg cggcaagcca ggcgccgata
      841 gccagccaac cgacgacacc gacaacagaa ccgtggccca ggacgaggtt gtcgccgagt
      901 cgacgtcgga cgatgtcgct gccgacacga tggagcctgc cgaaacggcg gacagcgaat
      961 ctcaatcgat ctagcccaat ttcgaatcgg gacgattggc tgatttcgct cccggtcgtc
     1021 aggcgtcacg cttgggaaaa gcgcgagccc actcgtcgag tggtcgattg tgcttgccga
     1081 ctgcctgact gactgcggag caccaccatg agcgccgacg ccgcgccagc agagacaacc
     1141 cagaagaccc tacgcattgg atccttcatt gcggcccgcg aatcggagca aggcgcgcga
     1201 accagtccga ttcccgtcat cggcgccgag gagaccgcct tcgccggtaa gccgggcctg
     1261 gcacattggc agcagatcgg gccaacgccc gacgcaatcg ccgaggcaga ggttaagctg
     1321 gcgaagcttc gccacctgag ccatcctgcc ctcgctcctg tcatcgaaag cgggctgaac
     1381 aagaccgtgg cctggtggat cgaagccgtt ccgcctggcc ggatgctcgc cgatgcggtc
     1441 cgggacgacg cgtcgctttc ttcgatcccg tcggtccggc gccttgtcga agttgtcagc
     1501 ggcgtgctcg cgctgacgca cgaggtcggc ttgtcgcacg gctcaatcag cgggaaatcg
     1561 atcgtgatcg gctccaacgg cgcgcccgta ctaaccggat tgggcatcgg cggaaagggc
     1621 ttcacacagg atcaggccga cctcgcgctc ctcgtcatca acctcctggc gggcgaaccg
     1681 tggaacgagc aagcggcgga tcccttgcgt cgtaccgacc agctcaggga gcatctgggc
     1741 aactacaccg atcggatttc cagcgtgctg gcgagagcca cgcatcctga tccagccgag
     1801 cgctattcct tgatcgccga ctttgcgaag cagtttggcg aagctgttca gttctccggc
     1861 gaggatctgg tgcaggccgg ccaggacgcg atctcatcgc agaatgccga gctggcgaaa
     1921 ttgttggccg aaaaggccgc agcatatgac ccaaactcgg acgcgctcgc caatctcaac
     1981 caccgcctcc gcggtggctt gccgttcgcg acaaatggcg cgcaagcgat gtccaacctc
     2041 gattccccga ttccggccct gccggggacg ctatccggtc tcaccccggt tgatcccgcc
     2101 gcgaacatgc gaaatcagct accgccggag ctgaccgaag gcttgcccca agagctgatt
     2161 gacctgattg cgccacagtt cgaggtcaag ccggtcaaga agggcgtacc gccgctcctg
     2221 gtgatgatcg tcggcttcgt cggcatcgcc ttcctgctgg cactggcgcg gatggtgaca
     2281 ctcctgatca ccggaaacta gccccgcgat cgcaggaaat cgtagacctc ggcccgattt
     2341 ggaagggacg tgaatgcgcc ggcggccgac gctgccagcg cgcccgccgc ggcaccgtag
     2401 cggagagacg cccggtcgtc gtcgccacgc tgccacgccg cgatcaacgc gccgagaaat
     2461 gcatcgccgg ccccgaccgt atccacgacc tcgattggcc aggctgcctg ttcaatgact
     2521 tggcttccgc gggcgaggac catcccctcc gctccgcacg taattaccgt cgcacccaat
     2581 ccgaatcgct cgttgagccg cgccgagacc agagtggcgt cggctcgatc tgaaatcggc
     2641 gccgcgatca agaccgccgc ttcatggcga ttgacgacga ggacatctgt gagatcgaga
     2701 acttccgcca gagcgtcggg gtcgacatcc tggatcggtg aggcattcat aaccgtggtc
     2761 gccccggcat cacgtgcgcg aatgaacgcg gcgatcgcaa gtttcgtccc caattccagt
     2821 tgcgcgagaa cgaaccgcga gcgccgaaat acgacctcgg cagcagccag gtgggtatcg
     2881 gtcaatccgg cggccgcgcc ggggacgatg atcgacgcat attccccgcc gacggcgaag
     2941 accgtacttg cgcccgttac ggcatcttcg ggccgggcaa ggaacgagag gtcgactcca
     3001 gacgcggcca attgaccggc tatgacgttt ccaagcggat ctctcccgat ccgtgagatc
     3061 agtacggtcg ggaccccgag cctggccaac tgtgcggcct ggttccccgc tttgccgccc
     3121 ggcgcaagcg tgaaccgatt cccgataacc gacgagcccg gcgtcggcat tgtcacggcc
     3181 gaggcgatca ggtccgtatg gacgctgccg acgacggtga catacggccg gtcgatcacg
     3241 gcatgaggcc ctggatcagc cgcatggcgc taaccgcatc ctgagcagcc tggatatcgg
     3301 ccaggagccc atcgtcgatc gaatccaggc gctctaccat ccgctggacc gtcgcgatgt
     3361 tggccgcgca ctctgccacc ggatccaagt gttccgggaa ggtgtcgaag tagatcgtgc
     3421 gattccaccc ggcccgcttg agcacgagca gcaactctac cgtctggacc ggatgaacgg
     3481 agcccaccat cagcccgtcg tccaggtgcc cgtagccatc gttcagatgg atgccgatca
     3541 gccggttctc gcggatcgcc aggccggcgg ccatagcggg cgactcgccg gccatcagcg
     3601 agtggcagaa gtcgagcgtg acgccaacat tgcccagtcc cgtatcccgg acaagcagaa
     3661 gcgcatcgct gatcccgcgc acgagcgaga agcggcgcgg atcactcggc ttgggctcaa
     3721 tgctgaccgt gatcgcgtca tgtcgcgccg cgacggcgcg cacgctatct accgcccatt
     3781 gccagagttg accgtattcg gcctgaaacg ggtagtcgaa accgtccgga ccgagccaga
     3841 ggatgacgtg gtcgacgtcg cggtcggcgg caatgtcgac ggcctgctgc gcgagggcaa
     3901 tcgccgcctc gcgaacttcg ggatcgggat tggtgaacga cccggccgcg aaccgtggcg
     3961 gatcgaaccg caggttgagc gcggtcaccg ccaagcccat ggagtgcgcc gcggtaatca
     4021 gcgtgcgatc ttccggaccg gagaagtgct gcgggtagtt cagctcagtg gccgtaatcc
     4081 ctgggaccgt gcttagcagg cgcagggcat cgagcggcga tcgggccggc gatactaccc
     4141 gaaagctgtt gagccgagaa gcgaatcgtg tgaactggaa cggcatatcg cgatcatcca
     4201 tagtgacgac ccaagttgac agtcttccgc agcgttgcat actatccgca gacttcagcg
     4261 tatgtcgaaa ggggttgtct ccccggcgga atcaggctca gatcggcgcg tcgccgcgcc
     4321 gttttcggct cgccggatgt cccgatcccg acgcacggac cggaagtcag gtttcgtcac
     4381 gcgatgtttg gagggaatgc agtggtctca aacgaaaaag atcgcatcct cggcgtgaaa
     4441 gctccccggc gcaccgtatt gaagggcgcc accggagcag cagggctggc cgcggcagga
     4501 atgatcggca agtctgcacc ccaggcgctt gcccaggacg acgtcaagtc acagattctc
     4561 gccatccccg gagcaggcaa gggtcagccg actgagcagg atatgcagaa ggttggcgag
     4621 ctgtgcatgg gtccgaccaa ggcgaacgtg cagcaaggcg agtttagcgg cgtcgagctg
     4681 acgttcatgg ggctcaacaa ccagaacctg cacaacatcg tcttccgcaa cctgctgacc
     4741 tcattcgtcg actacaccgg cgtcaagatc aactggatcg acctcgccca ggccgattac
     4801 aatccgcgcc tccagcaggc catcgcgacc aagacgatcg acttcgacgt cgtggagatg
     4861 ggagccccgt acgagggaga tgtctgcggc gccgggcttg cctccgagat gccggactgg
     4921 gtcaaagagc aaatcgacat gtccgactac gtcggctatc tgcagccacc tgtcggcacc
     4981 tggaacggca agacctatcg cgtctcgatc gacggcgact gccacaattt caattaccgg
     5041 accgactact tcagcaatgc ggatctcgcc tcggcctgga catcggaagg caaccagggc
     5101 gagtgggcag tgccgaagac ctatcagcag gttcaggccg tcaccaagtt cctgaagggc
     5161 aagcagatcg atgggcagga cgcgtacggg tggctcgatg tctgcaagcc gggcggcggg
     5221 tttagctggt acttcttcgc cagccgcgcg acggcctacg ccaagcatcc ggacgacaag
     5281 gcatggctgt tcgacgcgga tacgatgaag ccgcgcatca acaacccggc cttcgtgcgc
     5341 gcgatccagg acgtgatcga tgcgctgcca tccgaacccg cagaccagat caatgccgac
     5401 ctgaacgtga ccggcttcag tgagttcctg ggcggaaccg gctcgatgtg cgcctggtgg
     5461 ggcgacgtcg gatccaacgt ctacacgaac gaccagtcgg tggttcagga caaggtcgcg
     5521 ttcgacatcc tgcccggatc ggacgatgtc tacaactcga agacgggcca gtgggacacg
     5581 ctttccagcg ggccgaacta tgcgccgaac atggcctacc tcggatgggg cgtatacgtc
     5641 atggcgcggg tcgacaccga tccagtcaag caaaaggcgg cctggagcgt ggctgcgttc
     5701 ctcggcggaa aagacgtctc gctctggctc gcggcctacc cgtccgggtt ccagccgtac
     5761 cgcaacagcc acttcaacgt ggccgactgg gttgccaccg gttaccctga agagttcgcg
     5821 aagagctacc ttgcctcaga gcagaactcc tataaccacc cgaacgcggc gatcgagcca
     5881 cgcatcccgg gtatcttcca gtactacagc gccgctgagg acgagctggc caaggccttt
     5941 gccggtcaag tcgacgccca aacatgcgcg gacaatatcg ccgccgcctg ggagaagatc
     6001 accgacgaca tcggccgcga cagccaactc gccctctacc agtcgtccct cgggatgtcc
     6061 ggtggcgcta cacccgtggc tagcccgagc agctagcacg cggcacaatt gacgttcatc
     6121 tgacggcccg gtggcgggca actcgccacc ggccgttgcc gcccgtgcgg agacgcgtct
     6181 gtatgcgttt cggttcgcct ggcatagctg gattgtgatg caggactcac cggccgtttc
     6241 gacatccgca tgggacgcgg gcagcgccgc cgttccttcg agccggcaac gcaccgggca
     6301 ggcgttgatc tcggcggcga ctgtcggcct gctcgcaatc gtttctatcc aaacgctcca
     6361 taccgtcaat gtgctcgatg cgggcttcgc gaattggcga cccgtgctgt acgcgtatgt
     6421 ggcctgggcg ctcgcccttt gtgctggtct ggttctttct cgcggcgagc atggacggag
     6481 ggcgatcttc gtcctgcctg cagtgctctt caccgtatcg atggtcattt tcccgaccgt
     6541 gttcggtctc tatatcgcct ttaccgactg gaacctgagc tcccggagcg ggcgccactt
     6601 cagcggtctc gacaatttcc gccagctcat ccatgactcc tactatcgaa attcgttgat
     6661 caacatgatc tattacgtgc tgagcgtgtt cgttcagtac gccattgcgt tcgggctcgc
     6721 actcttgctg aatgccgacg tccgcggacg caagttcttc cgggtgacgt tcctgctgcc
     6781 gttcatgttg agcccggtcg cggtcagctg gatgatcggc aagtcgatga tggacctgcg
     6841 tttcggaccc gtgtccgacc tggcccgccg cctgggctgg gacacaccgg cattctttgg
     6901 ctccgcctgg ccggcgcggc tgagcatcat ggtaatggac gcgtgggtct cgatcccgtt
     6961 catgatggtc ttgctcctgg cgggtcttca ggcgcttccc ggcgaagtgc tcgaatcggc
     7021 gaaagtcgat ggtgcgaccg ggtggaagtc gttctggcaa atgacatttc cactgctgct
     7081 gccggtcagc atcaccgcgg tggtgctgcg aatcatcttt gagctcaagc tcgcggacat
     7141 tgtcatcacg atgaccggcg gcgggccggg tggcgcgacg gataccgtga caagcttcat
     7201 atatcgcgaa taccgggatc gctcgaacgt c
//
LOCUS       DATAOZ010000136         6431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4263892, whole genome shotgun sequence.
ACCESSION   DATAOZ010000136 DATAOZ010000000
VERSION     DATAOZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4263892"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VHR64_03760"
     CDS             complement(<1..1268)
                     /locus_tag="VHR64_03760"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX3301971.1"
                     /translation="MPATIIMGGQWGDEGKGKLTDALAGRARMVVRANGGANAGHTVQ
                     TDRGTFKMHLVPSGILNPDCECVIGAGVVLDPVSLLTELDGLRQQGIGSDRLRISARA
                     HVVLPYHAAFDRAQEDRLEGSKIGTTLRGIGPAYSDKAARLGLRVADLIEPDVVSTRL
                     RQLIDLKNRQLELLYGAPMVDADGIIETYRAAGERLRPYVCETEPLVYDALSTGQQVL
                     VECAQGAMLDIDYGTYPFVTSSSPSAAGACQGAGIPPTFVERVIAVFKAYSTRVGSGP
                     FPTELHDETGQLIRERGREYGTTTGRPRRTGWFDAIAARHVVQLNGVTEITMTLLDVL
                     DALEDIQVCTSYAANGETLDFIPASTERLAHVTPNYESRPGWVSDITGARSASDLPSR
                     ALEYVHFIEGLVGAPITMVGVGPAREQLVSL"
     gene            complement(1361..2395)
                     /locus_tag="VHR64_03765"
     CDS             complement(1361..2395)
                     /locus_tag="VHR64_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX3301972.1"
                     /translation="MASSQLKDHYQTLGVSRTATEKELKAAFRKLARKYHPDTNKDNP
                     EAEEQFKRINEAYEVLSDEKDRKLYDRYGDEWRAYRDAGFTGDEPRSQSRPGGQPPFY
                     RSYTYEGGSEQPDEGMFGSIFENLFSRGGRTSTTGFERRPVKGTDLEQSIDVSFDEAF
                     RGTERRFDIQSPDVCPTCHGSGTVRGAICPTCDGTGTVPRSRTIEVSVPAGVTSGQRI
                     RVKGQGSRSASGGPAGDVYLIVNVLPDSRFERDGANLKTTIDVPLLDAVLGGEAHVPT
                     PTGRVALTIPAETQNGRVFRLRGQGMPKVKTKGERGDLLATVRVVLPQHLSDQERALF
                     LQLRDAHGGR"
     gene            complement(2441..3037)
                     /locus_tag="VHR64_03770"
     CDS             complement(2441..3037)
                     /locus_tag="VHR64_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX3301973.1"
                     /translation="MNSGIFAGIGLAAACGWNTFTPLLVLALADRIVGGDLLNRPYEF
                     ISSVGGILILLALMTVELVGDKVPRLDHALDVLGAVLRPTTGALCFMAITNHDSSMHP
                     ILAMVFGLVVGGVVHWDKVQRRIHLAHQGLGLGTPFVSVVEDFASMITAICAVLYGIL
                     GPIAAVLSWLMVRATYKWGENFGKGTIARARERQTLRR"
     gene            complement(3122..3760)
                     /gene="lexA"
                     /locus_tag="VHR64_03775"
     CDS             complement(3122..3760)
                     /gene="lexA"
                     /locus_tag="VHR64_03775"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX3301974.1"
                     /translation="MKELSRRQNLILEFIETFLVENDYPPTIRDIQRELGISSTSVVD
                     YNLKILEGRNLIRRNRNISRGIELVNRTSISRNVVRIPVIGQIAAGLPIPVPGDLEGA
                     EHTETIELSHHLFADNAQDLFALRVKGLSMVDALINDGDIVLLRQQATCENGETVAVW
                     LKDERETTLKKFYHEGNRIRLQPANTTMEPIYTAPGNVEIQGKLVTVIRPMA"
     gene            3978..4535
                     /locus_tag="VHR64_03780"
     CDS             3978..4535
                     /locus_tag="VHR64_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX3301975.1"
                     /translation="MNGLDFAIIFAFLAIIGLGFFGGIVRVAAAIVAIYVGSIVSAAF
                     YDRLTDEFRQHVASVSVQMGQLFVFCALFLVSSAAIWWLLSTTVKGMKIKRRFEIIDN
                     LGGAMLGVAVSMLAVTLAAMLLSILLQVLNRTVGAGDSAALVGTVQGQIRHSRLVPVF
                     LDLTPYFTRVIEPWFPNGLPALLRP"
     gene            4565..>6431
                     /locus_tag="VHR64_03785"
     CDS             4565..>6431
                     /locus_tag="VHR64_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX3301976.1"
                     /translation="MANQALSVRKLELPAILDRLASLCQFSLGSARARELGPSGEPGQ
                     VAYLLDVTSEALELLGDQPDLTVGGARDIRSHIERAGKGSRLQPTDLLLVADTLHAVH
                     TFRRTLQRIQAGDERFPRLFEFAEGLANLTALETTLNRTVSARGDILDTASEELGRFR
                     REARVAQGRLTERLNSMINGGKYSSAVQDAIVTMREGRYVIPIRADARTQVAGIVHDV
                     SASGQTLFVEPLEIVDLNNTWRESKVREAREIERILDVVTGEIGASAPLLLSSIEALA
                     AFDVAFAKAKLAMQMAAVRPEIASDHDRSIRLIRARHPLLDPKTVVPIDIEVGGSFRL
                     LVITGPNTGGKTVALKTAGLLSLMAQTGLFIPADQGSQLPVFEALFVDIGDEQSIQQS
                     LSTFSSHMTNVISVLQSVGRQDLVLLDEVGAGTDPQEGSALARALLDEFLKTGALVIA
                     TTHYTEVKAYASTTAGVENGSVEFDLKTLSPTYRLSIGMPGQSNALAIAKRLGLPQPI
                     VESARQLLDPDAVRTEHYLSEIRARRSDAERTLGRARDVEREAKQLRRLAREELRAAE
                     EQRRTAREDALAEAEVELAEIRNLSRRLERERFQAAADREEVEARRRSIESAQQTV"
BASE COUNT         1130 a         1973 c         2091 g         1237 t
ORIGIN      
        1 agggaaacca actgctcgcg cgccgggccg acgccgacca tcgtgatcgg cgctccgacc
       61 aatccttcga tgaagtggac gtattcaagc gcgcgggatg gcaggtcgga cgccgaccgg
      121 gcgccggtga tatcggacac ccatccgggc cgggattcgt agttcggcgt cacgtgagcg
      181 aggcgctcag tgctggcagg gatgaagtcg agtgtttcgc cgttggcagc gtagctggtg
      241 cagacctgga tatcttcgag cgcgtcgagc acatcgagga gagtcatcgt aatctcggtc
      301 acgccattga gctgcacaac atgtcgcgcg gcgattgcgt cgaaccagcc cgtccgccga
      361 ggacgaccgg tcgtggttcc gtactcgcgg ccgcgctcgc ggatgagttg accggtctcg
      421 tcatggagct cggtcgggaa tggcccactg ccgacccggg tgctgtacgc cttgaagacg
      481 gcaatgactc gctcgacgaa tgtcggcgga atccccgcac cctggcaagc gccggcggca
      541 gatggggacg atgaggtaac gaacgggtag gtgccgtagt cgatatcgag cattgcgcct
      601 tgtgcgcatt cgaccagcac ctgctggcca gtgctcaacg cgtcgtagac cagcggttcg
      661 gtttcgcaga catatggccg cagtcgctcg ccggccgcgc ggtacgtctc gatgatcccg
      721 tcggcatcga ccatcggcgc tccgtaaagc agttccagtt gccggttttt gaggtcgatc
      781 aattgccgga ggcgggtcga gacgacgtca ggctcgatca gatcggcgac gcgcaggcct
      841 agtcgggcgg ccttgtcgga gtaggctgga ccgatgccgc gcaaggtcgt gccgatcttg
      901 ctgccttcga gccgatcttc ctgcgcgcgg tcgaacgcgg cgtgatacgg aagcacaaca
      961 tgcgcacggg cggagattcg gaggcgatca gagccgatgc cctgttgcct caggccgtcg
     1021 agctcggtca ggagactgac cggatcgaga acgacccctg cgccaatcac gcattcgcaa
     1081 tcagggttga ggatgccgga ggggacgagg tgcattttga atgtgccgcg gtcagtctgg
     1141 accgtgtgac cggcattggc gccaccgttg gcacggacga ccatccttgc gcggccggcc
     1201 agcgcgtcgg tgagcttgcc tttgccctca tctccccatt gtccgcccat gatgatcgtg
     1261 gcgggcatct cgatcctccc cggtttggtc gtgctctgtg tgcgtgaacg gtcgcagcga
     1321 atacgtgcgg cgcacccggg gaactatacc cgagggtcag ctaccgcccg ccgtgcgcgt
     1381 cgcgcagctg caggaagagc gcccgctcct gatcgctcag gtgctgcggc aggacgaccc
     1441 ggacggtcgc gagcagatcg ccgcgctcgc cctttgtctt gaccttgggc attccctgac
     1501 cccggagtct gaagacgcgg ccattttgcg tctcggccgg gatggtcagt gccacgcgtc
     1561 cggtcggggt tgggacgtgc gcttcgccgc cgaggacggc atcgagcagg gggacatcga
     1621 tcgtggtctt gaggttggcg ccgtcgcgtt cgaatcgcga atcggggagc acgttgacga
     1681 tcagatagac atcaccggcg gggccgccgg atgccgaccg actgccctgc cccttgaccc
     1741 gaatccgctg gccggaggtc acacctgctg gaaccgaaac ctcgatagtg cgactccgag
     1801 ggactgtccc tgttccgtcg caggtcgggc agatcgcgcc gcggaccgtt ccgctgccgt
     1861 ggcaggtggg gcaaacatcg ggcgactgga tatcgaaccg tctctctgtg ccccggaatg
     1921 cctcgtcgaa ggagacatca atcgattgct ctagatccgt tcccttgaca ggacggcgct
     1981 caaatccggt tgttgaggtg cgaccgcccc gggagaagag gttctcaaag atcgagccga
     2041 acatgccttc gtcgggctgc tcggacccac cttcataggt gtagctccgg tagaacggag
     2101 gttgtccgcc agggcgactc tgcgagcgag gctcatcgcc ggtgaagccg gcgtctcggt
     2161 aggcgcgcca ttcgtctcca tagcggtcgt agagcttgcg atccttctcg tcgctgagaa
     2221 cttcgtacgc ctcgttgatg cgcttgaact gctcctcagc ttcgggattg tccttgttgg
     2281 tgtccgggtg gtacttccgc gccagtttgc ggaaggcagc cttgagttcc ttctcggtcg
     2341 cagtgcgcga gacgccgagt gtttggtaat ggtctttgag ttgagaagac gccatggccg
     2401 ctccctcatt cttcatagac catatgataa acgttgagtc ttatcgacgc aaggtttgtc
     2461 gctctctggc gcgcgcgatg gtgcccttgc cgaagttctc gccccacttg tacgtcgctc
     2521 ggaccatcag ccagctgagc accgccgcaa ttggcccgag gatgccgtat aggacggcgc
     2581 agatggcggt aatcatcgag gcgaagtcct cgactacgct cacaaacggc gtacccagac
     2641 cgagcccctg gtgtgcgagg tgaatgcggc gctggacctt gtcccaatga acgactcccc
     2701 cgacgacgag cccgaagacc atcgcgagga ttggatgcat cgatgaatca tgattggtaa
     2761 tggccatgaa gcagagtgcg ccggtcgttg ggcgaagcac cgcgcccagg acatcgagcg
     2821 cgtgatcgag tcgggggact ttatcgccga ccagctccac ggtcatgaga gcgagcagga
     2881 tgaggatgcc gccaaccgag gagatgaatt cgtacgggcg gttcagcagg tcgccgccga
     2941 cgatgcggtc ggccagggcc aaaacgagga gcggcgtgaa ggtgttccaa ccgcacgccg
     3001 ctgccaggcc aatcccggcg aagattcctg agttcaaaag cgcttcctga cgtgcgtcga
     3061 tcgttcagtc gaatgccgtc cggaatcatg ccgcattcgg tcgcgcggcg tcgatctgcg
     3121 cctacgccat cgggcggatc acggtcacca gtttgccctg gatctcgacg ttgcccggtg
     3181 cggtgtagat cggctccatg gtggtgttcg ccggctgcag tcgaatgcga ttgccctcgt
     3241 gatagaactt cttgagggtt gtctcgcgct catccttgag ccagaccgct acggtctcgc
     3301 cgttctcgca cgtagcctgc tggcgaagca ggacgatgtc tccatcgttg atcagcgcat
     3361 cgaccatcga gaggcccttc acccgcaggg cgaacagatc ctgcgcattg tcggcgaaga
     3421 ggtgatggct gagctcgatc gtctcggtgt gctccgcgcc ctcgagatcg cccggaacgg
     3481 gaatcggcaa accggccgcg atctggccga tcaccggaat ccggacaacg ttgcggctga
     3541 tgctggttcg attgaccagc tcgatgccac gcgagatgtt gcggttgcgc cggatcaggt
     3601 tacgcccctc gaggatcttc aggttgtagt cgaccacgct cgtcgaggag atgccgagct
     3661 cgcgctggat gtccctgatt gtcggcggat agtcgttctc gaccaggaac gtctcgatga
     3721 actcgaggat gagattttgg cggcgggaga gttctttcat tagggtctcc agggcgaaag
     3781 agacggcagc atgcgcggaa tgcctgttac tagaacaaga atacgaacga acacgtgttc
     3841 gtgtcaagca tctttggggc tttcggggga tacaacagcc ggcctctggt tcttagtgtc
     3901 agggcagcgc ttcgatttgg gcacaaacgg ccgtcggaac tcagcgcctg acacacgctc
     3961 gagcggaagg accagcgttg aacggtctgg attttgcaat catcttcgca ttcctggcca
     4021 tcattgggct tggcttcttc ggcggcattg tccgtgtggc ggccgcgatc gttgctatct
     4081 acgtcggctc aatcgtgtcc gcggccttct acgaccgttt gaccgacgag ttccggcagc
     4141 atgtcgcatc ggtcagcgtg cagatggggc agctctttgt cttctgcgcg cttttcctgg
     4201 tgtcgtccgc ggcgatctgg tggctgctgt cgacgactgt caagggaatg aagatcaagc
     4261 gtcgcttcga gattattgat aatcttggcg gcgccatgct tggcgtcgca gtctcgatgc
     4321 tggctgtcac gctggcggca atgttgcttt cgatcttgtt gcaggtactt aaccgaacgg
     4381 tcggcgccgg ggactctgca gccctggtcg gaacggttca gggccagatt cgacactccc
     4441 ggctcgtgcc cgtctttctg gacttgactc cgtacttcac ccgggttatc gagccgtggt
     4501 tcccgaacgg gctgcccgcg ctcctgcgcc cctgaggtaa gtgccgcgcg tcggggagac
     4561 gcgattggcc aaccaagcac tctcagtccg aaaactcgag cttccggcga tcctcgatcg
     4621 tttggcctca ctttgccagt tttccctcgg cagcgcgcgc gcgcgtgagc tcggcccctc
     4681 cggcgagccc ggacaggtcg cctacctgct cgacgtcacc agcgaagcgc tcgagctgct
     4741 tggagatcag cccgatctga ctgtcggcgg cgcccgcgac atccgctcgc acattgagcg
     4801 agcaggcaaa ggctcgcggt tgcagccgac cgatctgctc ctggtcgccg acacgcttca
     4861 tgcagtccac acgtttcgcc gcacgcttca gcgcatccag gccggtgacg agcgatttcc
     4921 gcgactcttc gagttcgctg aggggcttgc caatctgacc gcgctcgaaa cgacgctgaa
     4981 ccggaccgtc tcggcgcgcg gagacatcct cgacaccgcg agcgaagaac ttgggcggtt
     5041 tcgccgagag gcgagggtcg cccagggccg cctcaccgag cgcctgaact caatgatcaa
     5101 cggcggcaag tactcctcag ccgtacagga cgcgatcgtg accatgcgcg agggccggta
     5161 cgtaatcccg atccgggccg atgcccgcac gcaggtggcc gggatcgttc acgacgtctc
     5221 tgccagcggc cagacactgt tcgtcgagcc gctcgagatc gtcgatctga acaacacatg
     5281 gcgcgagagc aaggtccggg aagcgcgcga gatcgagcgg atcctcgatg tcgtgaccgg
     5341 cgagatcggc gccagcgcgc cgctcctgct gagctcgatc gaggcgctgg ccgccttcga
     5401 cgtcgcattc gcgaaagcca aattggcgat gcagatggcg gcagtgcgcc ctgagatcgc
     5461 gtccgaccac gatcgctcga ttcggttgat ccgggctcgc cacccgttac tcgatcccaa
     5521 aaccgtcgtg ccgatcgaca tcgaggttgg cggctcgttc cgcttgctcg tgatcacggg
     5581 acccaatacg ggcggcaaga cggtcgcgct caaaaccgcc gggttgctca gcctgatggc
     5641 ccagaccggt ctcttcattc ctgccgacca gggcagccag ttgccggtat tcgaggcgct
     5701 cttcgttgat atcggcgacg agcagtcgat ccagcagagc ctctcgacat tttcgtccca
     5761 catgaccaat gtgatttcgg tcttgcaatc tgtcggtcgg caagatctgg tcttgctgga
     5821 tgaggtcgga gcgggaaccg acccgcagga aggctcagca ctggcccggg cgctgctgga
     5881 cgaattcctg aagaccggcg ccctcgtgat cgcgaccacc cattacacgg aggtgaaggc
     5941 gtatgcgtcg acgaccgcgg gcgtcgagaa cggcagtgtc gagttcgacc tgaagacgct
     6001 ctcgccgacc taccgcctct cgatcggaat gcccggccag tcgaacgccc tggcgatcgc
     6061 caagcggctc gggctcccac agccaatcgt ggaatcggcg cgccagctcc tcgatccgga
     6121 cgcggtcagg acagagcatt acctgtcgga gatccgtgcc cgccggtcgg acgccgagcg
     6181 gacgctgggc cgggcacgtg atgtcgagcg agaggcaaag cagctgcggc ggctcgctcg
     6241 cgaggagctg cgagcggccg aggagcagcg gcgaaccgct cgggaagacg cgctcgcgga
     6301 ggcagaggtt gagctggcgg agatccggaa tctctcccga cggctggaac gggagcggtt
     6361 ccaggcggcc gccgaccggg aagaagtcga ggcccggcgc cgctcgatcg aatcggctca
     6421 gcaaacggtg c
//
LOCUS       DATAOZ010000137         3271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8474898, whole genome shotgun sequence.
ACCESSION   DATAOZ010000137 DATAOZ010000000
VERSION     DATAOZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8474898"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..2501)
                     /locus_tag="VHR64_03790"
     CDS             complement(171..2501)
                     /locus_tag="VHR64_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3301977.1"
                     /translation="MPHSSRQLSNLPALLTTIFGREDEVAAVMSTIRQGRRLVTLTGP
                     GGVGKTCVAIAVGEALLDDFPDGVRFVSLAPLGDPDLVLPAIGRAVGVRDAGTSTISE
                     QLAGYLQDKRLLLILDNFEQVADAGPALTELLSACPNLSALVTSRMRLRLTGEVEHTV
                     PPLPLPDASDQASLEQVFDVPAIHLFVMRAQEIESSFMLTDDNVGAVAEICRRLDGLP
                     LAIELAAAWTRLLPPQVLVTRLEYRLPLLTGGGRDLPSRQKTIRDSIAWSYDLLSPGE
                     QQFFLRASVFVGGFTLDAIEAVAGESHDLAVLDGLAGLMESSLLRRYESGTGEPRYAM
                     LETIREFGLEHLRSQEEDHEIRARHARYFLALAEQAAPELLGADQVVWLDRLTVDHDN
                     LREAFIWLCTPATATDCLRLAGACGHFWYVRGYMREGRARLERALELAEPGPTVAHAR
                     ALNELGRFVYVARELPAAASIAREALAIWRAVDDRAGEAQALYIIAMTEENQLRWESA
                     KPLYEEALAIWRELDDPFRIGDVLSLLGGVAYGQGNLAQARALEREAAAIFRDVGDLR
                     RESLCSWYLGMFAACHGQWAEAARKYRESLRGLTEVRDASWLAKPIIGLAATAGTCGL
                     PDSAARLLGAADGLLDRIGATLTPFDIPAHELAHNSARSTLGELDFAAAIAVGRNLDV
                     EDVLLEADTIVAAAEAVPAEPLRRGAGTLGELTAREREVLRLLADGRADREIGEVLFI
                     SHRTVNAHVASILAKLGVSSRREAGAISHDLDTLPM"
     gene            2655..>3271
                     /locus_tag="VHR64_03795"
     CDS             2655..>3271
                     /locus_tag="VHR64_03795"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HEX3301978.1"
                     /translation="MERSKHRRHTLTVEEAVRRILLGCQVLPERAVPLLEARGLVLAR
                     DVVAQSPSPRFDTAAMDGYAVQHADVRGASPERPVHLGVVGSVAAGVRDAAAVAPGTA
                     VRIMTGAPCPEDADVVIPFELVEVRDDLIVVSQPFSAGVNIRRTGEDIAVGSIVVESG
                     TMLGPAHLAAIAAEGHETIEVMPRPRVAVIATGDELVRPGQALEPG"
BASE COUNT          594 a         1011 c         1092 g          574 t
ORIGIN      
        1 ccggtcaggg taaccagccg gcggccctgc ctgatggtgg acatcaccgc cgcgacttcg
       61 tcgtcacgtc cgatgagcgt ggtcagcaat gccggtagat tggacagttt gcgactggag
      121 agaggcatgg catcccggtc ccagcgctat ccaaatgcgg tttgtctatt ttacataggt
      181 aatgtatcga ggtcgtgact gattgcgcct gcttcgcgcc gagaagacac gccgagtttc
      241 gccaggatgc tcgccacgtg ggcgttgacc gtgcgatggc tgatgaagag tacttcgcca
      301 atttcgcgat cggcacggcc gtctgccagg agtcgcagca cctcgcgctc ccgggcggtc
      361 agttctccga gtgtccctgc gccccgccga agcggttcgg cgggaaccgc ctcggctgcg
      421 gcgacgatgg tgtcagcttc gagcagcaca tcctcgacgt cgaggttgcg gccaacggca
      481 atcgccgcag caaagtccaa ctcgccgagt gttgaacggg cggagttatg tgccagctcg
      541 tgggccggga tatcgaacgg cgtcagggtg gcgccgatcc ggtcgagcaa tccgtcggcc
      601 gcgccaagaa gccgtgccgc ggagtcgggc aggccacacg tgccggctgt ggcagccaat
      661 ccgatgatag gtttggcgag ccaggaggcg tctctgactt cggtcagtcc gcgcagactc
      721 tcgcgatact tgcgggcagc ctcagcccat tgtccgtgac aggctgcaaa catgccgagg
      781 taccatgagc aaagtgattc gcgacggaga tctccgacgt cccggaagat ggccgccgct
      841 tcacgctcga gagctcgcgc ctgtgccagg ttgccctgtc cgtaggcgac tccgccgagc
      901 agcgaaagca catcaccgat tcggaacgga tcgtccagtt cgcgccagat tgccaatgcc
      961 tcttcgtaca ggggttttgc cgattcccag cggagttgat tctcctcagt catggcaatg
     1021 atgtaaagcg cttgcgcttc gccagcccgg tcatcgacag cgcgccagat tgccagtgcc
     1081 tccctcgcga tggaggctgc ggccggaagt tcacgggcga cgtagacgaa ccggcctaac
     1141 tcgttcaggg cgcgggcgtg ggctacggtt gggcccggct ctgccagttc cagcgcacgc
     1201 tcaagccgcg ctcgaccctc ccgcatgtac ccgcgcacat accagaagtg gccgcaggcg
     1261 ccggcaaggc ggagacaatc ggttgccgtg gccggtgtgc acagccaaat gaacgcctct
     1321 cggagattgt cgtggtccac agtgagtcga tccagccaga caacctgatc cgcgccaagg
     1381 agctccggcg ccgcttgttc ggccagcgcg aggaagtacc tggcgtgcct ggcgcggatc
     1441 tcgtggtcct cctcttggga acgaaggtgt tccaggccga actcgcgaat ggtttcgagc
     1501 atggcatagc gtggttcccc ggttccgctc tcgtaccgac gaagcaggct gctttccatc
     1561 aaccctgcga gaccatcgag caccgcaaga tcgtggctct caccggcaac agcctcgata
     1621 gcatcgagcg tgaatccacc cacgaagacg gaggcgcgaa gaaagaactg ctgctcgcca
     1681 ggggagagaa gatcgtaact ccaggcgatc gagtcacgaa tcgttttctg ccggctcggc
     1741 aaatcccgcc ctccaccagt caacaatggc agacgatatt cgagccgggt gaccaggacc
     1801 tgtggaggca ggagacgggt ccaggctgcg gccagctcga ttgccagggg caggccatcc
     1861 agtcggcggc agatctcggc caccgcgccg acattgtcgt ccgtgagcat gaagctggat
     1921 tcgatctctt gggctcgcat gacgaacagg tggatcgccg ggacatcgaa gacctgctcc
     1981 aaactggcct ggtcgctcgc gtctggcagc ggcaacggcg gaacggtgtg ctcgacctca
     2041 ccagtgagac gaagccgcat gcggcttgtg acgagcgcag acaggtttgg gcaggcggag
     2101 aggagctccg taagtgccgg accggcgtcg gccacttgct cgaagttgtc gaggatcaga
     2161 agtagacgtt tgtcctggag gtaacccgcg agttgctcgg aaatcgtact cgtgccggca
     2221 tcccgaacgc cgaccgctcg accaattgcc ggtaatacca ggtcggggtc cccgaggggg
     2281 gccagactga cgaagcgcac cccatcggga aagtcatcga gcaatgcctc gccgaccgcg
     2341 atcgcaacgc aggtcttccc gacgccgccc ggcccggtca gggtaaccag ccggcggccc
     2401 tgcctgatgg tggacatcac cgccgcgact tcgtcctcac gtccgaagat cgtggtcagc
     2461 aatgccggaa gattggacag ttggcgactg gagtgaggca tagcgtcccg gtcccagcgc
     2521 gatcccgatg cagttttctc attctacaga tggaacaaga tatgccgggt accctagatt
     2581 cagcggcctc ggcaatgacc gtccgggggc gtgggactgg gaacgggtcg gcttgcctgc
     2641 gatacagtga cgacatggaa cgctcgaaac atcgccgtca cacgttgacc gtcgaggagg
     2701 cggtacgacg gatcttactc ggctgccagg tgttgccgga gcgggccgtg ccgttactgg
     2761 aggcgcgcgg gctggtgctg gcgcgggatg tcgttgccca gtcgccgtcg ccccggttcg
     2821 acaccgcggc gatggacggg tatgcggtcc agcacgccga tgtccgaggc gcatcgccgg
     2881 agcggccggt acatctcggc gtggtcggct cggttgccgc cggagtgcga gatgcggccg
     2941 cagttgcgcc cggcacggcg gtccggatta tgaccggggc gccctgcccg gaggacgccg
     3001 atgtcgtgat cccgttcgag ctggtcgaag tgcgtgacga tctgatcgtc gtctcacaac
     3061 ccttttcggc cggcgtgaac atccggcgga ccggcgagga catcgcggtc gggtcgatcg
     3121 ttgtcgagag cggaacgatg ctcgggccgg cgcatctggc ggcgattgcg gccgagggtc
     3181 atgagacgat cgaggttatg ccccgcccgc gggtggccgt gatcgcaacc ggcgacgagt
     3241 tggtccggcc ggggcaagcg ctcgaacccg g
//
LOCUS       DATAOZ010000138         6090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2161017, whole genome shotgun sequence.
ACCESSION   DATAOZ010000138 DATAOZ010000000
VERSION     DATAOZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161017"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VHR64_03800"
     CDS             <1..250
                     /locus_tag="VHR64_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301979.1"
                     /translation="TPVAESGTPIAEDEVEDGGSDKPVSVKVDIDEDGNGTIEYDLDG
                     DGETDLTMIVEDGKVVEYVDAGDAADATPEATPDTATE"
     gene            complement(312..611)
                     /locus_tag="VHR64_03805"
     CDS             complement(312..611)
                     /locus_tag="VHR64_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301980.1"
                     /translation="MDVRRICLQIVVAVMIGLGSAGIHQVDAQGGQGDCVREFFRIAR
                     RDYGWSPHDVVVQLNSDNDRSYQLTGVHDLRTHVADGSQGIVDKETGETFSCPAS"
     gene            complement(783..2951)
                     /locus_tag="VHR64_03810"
     CDS             complement(783..2951)
                     /locus_tag="VHR64_03810"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX3301981.1"
                     /translation="MQHFSRRQFNRSLAFGAVALGAAMTPLTTRADGVIVVEPPECQT
                     GACPGPIRIGDQLTVKSHRVNVTVANQVATTTIDQVFFNQNDWVAEGTYIFPIPDEAT
                     VSKFTMIVDGQAIEAKVLTAEEAAAIYDQIVRSMRDPALLQYVGQGAIQASVFPIPPG
                     ESREVKISYEQVLTAEMGMVRYVYPLNTEQFSAQPLEQVSVRVEVTSADPIRAIYSPT
                     HQVAITQDSETHFVAGWEASNIWPTDDFALIYTVATDQIGANLLSYVDPITSEGFFML
                     LAAPGIGSNQAVVAKDVIVVLDTSGSMEGEKIVQAKQAVSYVLNHLNTEDRFAVIEFS
                     TGVRFYDHQLRAASEAPVAASWVETLPATGGTDINGALLEAMKMADRERPTYVIFLTD
                     GLPTEGEVDSAVILSNVGTASPGNVRLFSFGVGDDVDTFLLDSLVQQENGVSSYVRPG
                     ERIDEEVSLFYAKVGSPVLTDLQLNFEAVNVQELYPNPLPDLYAGSQLVLVGAYAAGG
                     PTRVTLSGMVNGKATSYVYEGQALTTAPGGPNDFLPRLWATRKIGYLLTQIRLHGENP
                     ELIQAVVDLSVRYGIVTPYTSYLITEDDILTQQGRDRVVSQEAAAPAGDVSGDEAVSK
                     ADAQSQMSGANQAAPTAEPSQYGGGNAIKVVGSRTYVQQNGVWIDTAFDPSTMTTTKI
                     AFLSDDYFAFLKAHPELAGALALGDKVIVAAGNQFYEIVP"
     gene            3272..4066
                     /locus_tag="VHR64_03815"
     CDS             3272..4066
                     /locus_tag="VHR64_03815"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX3301982.1"
                     /translation="MFGECRRLVYLACVGLLGVALCAGLAVRPAAAQGVPGQLPGIVV
                     TGFGAASASAESAEVQIIVGPDMYVMSPSTGAVTANDVASIIDAVVATGVDRADIELF
                     VPAVNSVFSGPGGPGNAQLRFTIPNPTDSTMSELSDAVYKTAVTARLSIQHLGVRYTA
                     ADCASLQQAATDKAVADARARGERLAMSLNVELGEMVQATDALYGYGAGVPDSCASPA
                     KQTYYGPYGVGIAPPYDPTQAVEATANATITVTFEMQPAAGATPVS"
     gene            4268..4564
                     /locus_tag="VHR64_03820"
     CDS             4268..4564
                     /locus_tag="VHR64_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX3301983.1"
                     /translation="METTPTTDTRYQQARKRVEEIKGFYTHLIIYLVVNAFLFAIDFI
                     DGGSWWFYWPLLGWGIAVAIHGAVTFGVEGPLGKHWEERKIRELMDEDQQAHSA"
     gene            4591..5220
                     /locus_tag="VHR64_03825"
     CDS             4591..5220
                     /locus_tag="VHR64_03825"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX3301984.1"
                     /translation="MQASPYPVQFAVDYLDAPRDRLSVGLRIFYAIPILIVFGTVSGG
                     NWGWSSESGQAGAAGAGGLLAAGPALMIIFRQKYPRWWFDWNLELQRFLNRVTAYLAL
                     MVDTYPSTDEHQSVHLDYVYPDVPRDLNRWLPLIKWLLAIPHFIVLVFLNIAAFFVVV
                     IVWFTILFTGEYPPELFRFVEGVMRWNNRVVAYTITFVTDSYPPFRLSA"
     gene            5236..5442
                     /locus_tag="VHR64_03830"
     CDS             5236..5442
                     /locus_tag="VHR64_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3301985.1"
                     /translation="MWWQDLLWGFWNGLTAWIILIAHIFNAWEEYPFYDVARAGNWYD
                     AGFLIGAGSPFAGTFGASSRARST"
     gene            complement(5447..>6090)
                     /locus_tag="VHR64_03835"
     CDS             complement(5447..>6090)
                     /locus_tag="VHR64_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HEX3301986.1"
                     /translation="EHGFRVYADRDDNGLLICTWPKGGRPTVPVRYCDEVWTGIEYQV
                     AALCIMEGLVDEGMRILVDLRNRYSGERRNPYNEIECGDHYSRAMAGWSVLEAISGVR
                     YDATRREIRLAAAHGRSGSRVPIMTDTGWGMIQQETREARLLVEIHVSYGSIEIRSIT
                     VDDLDGDVVSATLGHDEIAVTVSSAGGRLTIALQDETSIDVDQTLALQVSRQA"
BASE COUNT         1022 a         1863 c         2022 g         1183 t
ORIGIN      
        1 gaccccggtt gccgagagcg gcaccccgat cgctgaggac gaggtcgagg acggcggatc
       61 cgacaagccg gtcagcgtca aggtcgacat cgatgaggac ggcaacggca cgatcgagta
      121 cgacctcgat ggtgacggcg agaccgatct gaccatgatc gtcgaggatg gcaaggtcgt
      181 ggagtacgtg gatgcgggcg acgcggctga cgcgacgccg gaagcgaccc cggacaccgc
      241 aaccgagtaa ctcttcaact tcaccgatga gatcgacgcc cggctgagct cagccgggcg
      301 tcggtttgtt atcaggacgc agggcaggag aaggtttcgc cagtttcctt gtcgacgatg
      361 ccctgcgagc cgtcggcgac gtgcgtgcgc agatcgtgaa cgccggtgag ctggtaagag
      421 cggtcgttat cggaattaag ctggacgacc acatcgtgcg ggctccagcc ataatcccgg
      481 cgggcgatcc ggaagaactc gcgcacgcag tcgccctgac caccctgcgc gtcgacctgg
      541 tgaatcccgg ccgatccgag cccgatcatg acggcgacca cgatctgcag acagatacgt
      601 ctgacatcca tggcgacctc ctttgtaggc gcagtccggc agtcaccgaa ccgcccatgc
      661 gggaaggtcc ccagcatagc cagattgttt gcgacgtcaa ccgtgtagcc tacagacgca
      721 agggatcagg aggcagggat caggggtcaa gcgtggccct attgactgtt cctgatccct
      781 gattacggga cgatctcgta gaactggttg ccggcggcga cgattacctt gtcgccgagg
      841 gccaatgcgc cggccagttc cgggtgggcc ttcaggaagg cgaagtagtc gtcgctcagg
      901 aacgcgatct tcgtggtcgt catcgtgctc gggtcgaagg cggtgtcaat ccaaacgccg
      961 ttctgctgga cataggtccg gctgccgacg accttgatcg cgttgccgcc gccgtattgg
     1021 ctcggctcgg cggttggcgc ggcctggttc gccccgctca tctggctctg ggcatcggcc
     1081 ttgctgaccg cctcatcgcc cgagacatcg cccgccggcg cggccgcctc ctgactaacg
     1141 acgcggtccc ggccctgctg ggtcaggatg tcgtcctcgg tgatcaggta cgaggtgtac
     1201 ggcgtgacga tcccgtagcg gacgctgaga tcgaccactg cctggatcag ctccgggttc
     1261 tcaccatgga ggcggatctg ggtcagcagg tagccgatct tgcgggtcgc ccagagacgc
     1321 ggcaggaagt cgttcggccc gcccggcgcg gtggtcaatg cctgtccttc atatacataa
     1381 gacgtcgcct tgccattgac catgccgctg agcgtgaccc gcgtcggacc gccggccgca
     1441 taggcgccga ccagcacgag ctgcgaaccg gcatagagat caggcagcgg attcgggtac
     1501 agctcctgca cgttgaccgc ttcgaagttg agctggagat cggtcaggac cgggctgccg
     1561 accttggcgt agaagagcga gacctcctcg tcgatgcgct cgccgggccg gacgtagctg
     1621 ctgacgccgt tctcctgctg gacgagcgag tcgagcagga aggtgtcgac atcatccccg
     1681 acgccgaagc tgaagagccg gacattgccc ggcgaggccg tgccgacatt cgagaggatg
     1741 actgcgctgt cgacctcgcc ctcagtcggc aagccgtcgg tcaggaagat gacgtaggtc
     1801 ggtcgctcgc ggtcggccat cttcatcgcc tcgagcagcg cgccgttgat atcggttccg
     1861 ccggtggccg gcagcgtctc gacccagctt gcggcgaccg gcgcctcgct ggccgcgcgg
     1921 agctggtggt cgtagaagcg gacgcccgtg ctgaactcga tcacggcgaa gcggtcctcg
     1981 gtattgaggt ggttgaggac atagctgacc gcctgcttgg cctggacgat cttctcgccc
     2041 tccatgctgc cggaggtatc gagcacaacg atcacatcct tggcgacgac tgcctggttc
     2101 gagccgattc ccggtgcggc caggagcatg aagaagccct cgctcgtgat cgggtcgaca
     2161 tagctgagca ggttcgcgcc gatctggtcg gtcgcgacgg tgtagatgag ggcaaagtcg
     2221 tcggtcggcc agatgttgct ggcttcccag ccggccacga agtgcgtctc gctgtcctgc
     2281 gtgatcgcga cctggtgggt tggcgagtag atcgcgcgga ttgggtcggc cgaggtgacc
     2341 tcgacgcgga cgctcacctg ctcgagtggc tgggccgaga actgctcggt attgagcggg
     2401 tagacgtagc gaaccatgcc catctcggcg gtcaggacct gctcgtagct gatcttgacc
     2461 tcccgggatt cgccgggcgg tatcgggaag accgaggcct ggatcgcgcc ctggccgacg
     2521 tactggagca gggccggatc ccgcatcgag cggacgatct ggtcgtagat cgcggcggcc
     2581 tcctcggcgg tcaggacctt ggcctcgatc gcctggccgt caacgatcat cgtgaacttc
     2641 gagaccgtcg cctcgtccgg gatcggaaag atgtaggtgc cttctgcgac ccagtcgttc
     2701 tggttgaaga agacctggtc gatggtggtc gtcgcgacct ggttggcgac ggtcacattg
     2761 acccggtgcg acttgaccgt gagctgatcg ccgatccgga tcggaccggg acaggcgccg
     2821 gtctggcact cgggcggctc gacgacgatc acgccgtccg cccgggtcgt gagcggtgtc
     2881 atagccgcgc cgagggctac tgcgccgaag gcgagcgacc ggttgaactg ccgacgggaa
     2941 aagtgttgca tctccatctt gccacctgcg ctggtccact gacctgtgag ggcgatccgg
     3001 caccggaccg ccgcgaaatc acaaccgagc ccgttcccct ggcaaagaga cgatgcgggc
     3061 gacggaaagg ttcccgtagc gggttgaggg ttaagggaac ggaaggagta gccagtagtc
     3121 agtagccggt ggccagccct tggtctttgg tctttggtct tgcgttccct ccactctcca
     3181 ccctcgacca tcaaccgatc ttgactttcc cattgaaggg cgtagggtgg atttcacaca
     3241 gccagtgtcg atttgcgcga aggaggagcc gatgttcggg gaatgccgtc gattggtcta
     3301 tctcgcgtgt gttggactgc tgggggtcgc gctgtgcgcc ggtctggcgg tccgaccggc
     3361 tgccgcacag ggagttcccg gccagttgcc cggtatcgtc gtgaccgggt tcggggctgc
     3421 atcggcctcg gcggagtcgg cggaggttca gattatcgtc gggccggaca tgtatgtgat
     3481 gtcgccttcg accggtgccg tgaccgccaa tgatgtcgct tcgatcatcg acgccgtcgt
     3541 cgctacgggc gtggacaggg cagatatcga gctgttcgtc ccggccgtga acagcgtgtt
     3601 cagtggtcca ggtggtcctg gtaacgcgca gctccgcttc acgatcccga acccgaccga
     3661 ctcgacaatg agcgaactct ccgacgcggt ctacaagact gcggtcacgg cccggctcag
     3721 cattcagcac ctaggcgttc ggtacaccgc tgccgattgc gcctcgcttc agcaggctgc
     3781 cacggacaag gctgttgccg atgcgcgggc gaggggcgag cgcctggcca tgtcgctcaa
     3841 tgtggagctc ggcgagatgg tgcaggcgac ggacgcgctg tatggctatg gcgcaggtgt
     3901 tcccgacagc tgcgcgagcc cggctaagca gacctactac ggcccgtacg gggtgggcat
     3961 agccccgccg tacgatccta cgcaagcggt cgaagcgaca gccaatgcga cgatcaccgt
     4021 gaccttcgag atgcagccgg cagccggagc cacgcctgtt tcgtaggcat tgggttttcg
     4081 ggactggcta ccggctacgg actacttttc cgttccttta acccttaact ctccaccctt
     4141 aacccgcttt gttgcgcggt tgttgcggcc cggattacgc ggcaatgcgg gtgcgatgcg
     4201 tgggctgtta gaccgggggt gcggataggt ggcgatcgtg ctgctgtccg cgtgaaagga
     4261 gggcgtgatg gaaaccactc ccaccaccga tacccggtat cagcaggctc gcaagcgggt
     4321 cgaggagatc aaggggttct acactcacct catcatctat ctcgtggtca atgcattcct
     4381 gttcgcgatc gacttcatcg acggcggcag ctggtggttc tactggccgc tgcttgggtg
     4441 gggcatcgcc gtcgccatcc acggtgcggt caccttcggc gtggaaggac cgctcggcaa
     4501 gcattgggag gagcgcaaga tccgcgagtt gatggatgag gaccaacaag cccacagcgc
     4561 ctgaatgcgc gttagcggga ggaggaaccg atgcaggcat cgccgtatcc cgttcagttc
     4621 gcggtcgact atctggatgc tccgcgtgat cgtctctcgg tcgggctgcg catcttctac
     4681 gcgatcccga tcctgatcgt gtttgggacg gtttctggtg gcaactgggg ctggtcctct
     4741 gaaagcggcc aggcgggcgc ggccggagcg ggcgggttgc tggcagccgg gccggccctg
     4801 atgatcatct tccggcagaa gtacccgcgc tggtggttcg actggaatct cgagctgcag
     4861 cggttcctca accgggtgac cgcgtatttg gcgctgatgg tcgataccta tccctcgacg
     4921 gacgagcacc agtcggtcca tctcgactac gtctatccag atgtcccgcg ggatctgaac
     4981 cgctggctgc cgctgatcaa atggcttctg gcgattccgc acttcatcgt gctggtgttt
     5041 ctgaacatcg cggcgttctt cgtggtcgtg atcgtctggt tcacgatcct gttcacgggc
     5101 gagtacccgc cggagctgtt ccgcttcgtc gagggcgtga tgcgctggaa caaccgggtc
     5161 gtggcgtaca cgatcacgtt tgtcacggat tcctacccgc ccttccgtct ctcggcctga
     5221 tcaggaggtg cgcgcatgtg gtggcaggat ctgctctggg gcttctggaa cgggctgacg
     5281 gcgtggatca tcctgatcgc gcacatcttc aatgcatggg aggagtaccc gttctacgac
     5341 gtcgcgcggg cgggcaactg gtatgacgcc gggttcctta tcggcgccgg ctcgccattc
     5401 gccggcacgt tcggcgcgtc cagtcgtgcc cgctctacct gatccctcaa gcctggcgag
     5461 agacttggag ggcgagcgtc tggtcgacgt cgatcgaagt ttcgtcctgc aacgcgattg
     5521 tgagccggcc gcccgcgctt gacacggtta ccgcgatctc atcgtggccg agagttgccg
     5581 acaccacgtc cccgtcgagg tcatcgacgg taatgcttcg gatttcaatc gatccgtagg
     5641 agacatgaat ctccacgagc aggcgagcct ctcgcgtctc ctgctgaatc attccccaac
     5701 cggtatcggt catgatcggg acccggctgc cggagcgtcc gtgggctgcc gcgagccgga
     5761 tctcccggcg cgtcgcgtcg tatcggacgc cgctgattgc ctccagcacg ctccagccgg
     5821 ccatcgcacg ggagtagtga tctccgcatt cgatttcgtt gtacgggttg cggcgctcgc
     5881 cagagtaccg gttgcgcagg tccaccagga tgcgcatccc ttcgtcgacc agaccctcca
     5941 tgatgcacag ggcggcgacc tggtactcga tgccggtcca gacctcgtcg cagtagcgga
     6001 ccggcaccgt cggacggccg cccttgggcc aggtgcagat cagcagacca ttgtcatcgc
     6061 gatcggcgta gacgcggaag ccgtgctcaa
//
LOCUS       DATAOZ010000139         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3212407, whole genome shotgun sequence.
ACCESSION   DATAOZ010000139 DATAOZ010000000
VERSION     DATAOZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3212407"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      25..129
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            284..1492
                     /locus_tag="VHR64_03840"
     CDS             284..1492
                     /locus_tag="VHR64_03840"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX3301987.1"
                     /translation="MNQFNDGSSYTPVFAAPARTSSRSAGRGHLSVVPPQRMADSGDE
                     GALQQVEIGPLDLELGGHLPSVTMAYRTWGELNAAGDNAVIVLHALTGDSKATGEGGW
                     WDPLIGSGRAIDTDRQFVICANLLGGCQGSTGPASTDPLTGRPYGMRFPLVTIGDMVN
                     AQRRLVEQLGITKLVAIGGSIGGFQALEWATRHPDMVAASAPIAATPALGPQGIALNE
                     AGRRAIMADPDWRGGEYANEGVFPADGLAIARMIAMVTFHSRESMEMRFARKPATRPS
                     LYPAFGGTFDVEGYVHYHGAALVRRFDANSHLYLTRAMDLYDMAREGGEAYWIDKIAA
                     PMLLIGIRSDWLFPPDAICQFAARTAAAGKDVRYDELDSPHGHDAFLKEWDQLTAVLT
                     PFLARFGGGA"
     gene            1597..>1942
                     /locus_tag="VHR64_03845"
     CDS             1597..>1942
                     /locus_tag="VHR64_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX3301988.1"
                     /translation="MSDTTEKPLGFETLALHAGQDPDPNTLSRAVPLYQTTSYVFKDP
                     EHAARLFGLQEFGNIYTRIMNPTTDAFEQRVAALEGGVAGLAFASGQAAETAAILNIA
                     RAGDDIVSTTSLY"
BASE COUNT          338 a          639 c          630 g          335 t
ORIGIN      
        1 ccatcgcccc aacaaccaaa tactgcctta tcagacgggt ggagggagac ggccctgtga
       61 aacccggcag cctgcagcgt agttcgctgt aaggtgctaa ctccggcaga gcaatctgcg
      121 agataaggga tcagcgaaac gttcgccccc tgctcttgca gggggatttt tattccccca
      181 tgtagcaaga caaccgcagc tcacgtctgt gatcgattgg cagtctccgg caacgccgtg
      241 ccgcgactgg ttggcattgc catatgcgtg gggaaacgcg cgaatgaatc aattcaatga
      301 cgggtcatcc tacacgccgg tcttcgccgc gcccgctcgc acgagttcgc gttcggccgg
      361 tcgaggtcac ctgagcgtgg ttccgccgca gcgaatggcc gattccgggg atgagggcgc
      421 cctccagcag gtcgagatcg gaccgctcga tctcgagctc ggcggccacc tgccgtcggt
      481 aacgatggcg taccggacct ggggcgagct caacgccgcc ggggacaacg cggtgatcgt
      541 gctccacgcg ctgaccggcg acagcaaggc gaccggagag ggcggctggt gggatccact
      601 gatcggctcc ggtcgggcga tcgacaccga ccgccagttc gtcatctgcg cgaatcttct
      661 cgggggctgc cagggctcga ccggcccggc gtcgaccgac ccgctgaccg gcaggcccta
      721 cgggatgcgc ttcccgctcg tgaccatcgg cgacatggtc aacgcccagc gccgcctggt
      781 cgaacagctt ggcattacga agctggtcgc gatcggcggc tcgatcggcg gattccaggc
      841 gctggagtgg gctacccgtc atccggacat ggtcgccgcg tcagccccga tcgccgctac
      901 gccggcgctc ggcccacagg gcatcgcgct caacgaagcc ggtcgccgtg cgatcatggc
      961 cgatcccgat tggcgcggtg gcgagtacgc caatgagggt gtctttcctg cggacggcct
     1021 tgctatcgcg cggatgatcg cgatggtgac cttccacagc cgggagagca tggagatgcg
     1081 gtttgcccgc aagcctgcca cccgtccgag cctctatcca gccttcggcg gcaccttcga
     1141 tgtcgagggg tacgtccact accacggtgc ggccctggtc cgccggttcg acgccaacag
     1201 ccatctctac ctcacccggg ccatggatct ctatgacatg gcgcgggagg gtggcgaggc
     1261 gtattggatc gacaagatcg cggcgccgat gctgctgatc ggcatccgca gcgactggct
     1321 cttcccgccg gacgcgatct gccaattcgc cgcgcggact gccgccgcgg gcaaggatgt
     1381 ccgatacgac gagctcgatt cgccccacgg gcacgacgcc tttctgaagg agtgggacca
     1441 gctgaccgcg gttctgactc ccttcctggc gcggttcggc ggaggggcct gacacacact
     1501 gaatgcacta gcgccggcct ccacggcgcc tgcagatacg tcttcaggat ggatgatccg
     1561 ctgcatcgcg gcggatcgga aagggaaacg cgcaccatgt cagacacgac cgagaagccg
     1621 ttggggtttg agacgctggc gctacatgcc ggccaggatc ctgatccaaa cacgctttcg
     1681 cgggccgtgc cgctgtatca gaccacgtcg tacgtcttca aggatccgga gcacgccgct
     1741 cgcctgttcg gtttgcagga gttcggcaat atctacaccc ggatcatgaa tccaacgact
     1801 gatgccttcg agcagcgagt ggcggcgctc gagggcggcg tggccgggct ggcctttgcc
     1861 tccgggcagg cggccgagac agcggcgatc ctgaacatcg cccgcgccgg cgacgacatc
     1921 gtctcgacga ccagtctcta cg
//
LOCUS       DATAOZ010000140         8049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2688095, whole genome shotgun sequence.
ACCESSION   DATAOZ010000140 DATAOZ010000000
VERSION     DATAOZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2688095"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHR64_03850"
     CDS             <1..198
                     /locus_tag="VHR64_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3301989.1"
                     /translation="GLDVVRDIEWSYQRETLDPTDRPSPGLAELIDEGKLGEKSGQGF
                     YSHPNPAYSQPGFIPQRKKEE"
     gene            204..524
                     /locus_tag="VHR64_03855"
     CDS             204..524
                     /locus_tag="VHR64_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEX3301990.1"
                     /translation="MYHVVFLVTRKPDMSQDEFTDYWINRHTPLTARLPGLREYRCYP
                     MIATMGDQQPPFDAVAYLAFDDEASCRAALESKEFTTAVGDGVNFQNVDDTFGFYARE
                     YRIV"
     gene            complement(591..1166)
                     /locus_tag="VHR64_03860"
     CDS             complement(591..1166)
                     /locus_tag="VHR64_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX3301991.1"
                     /translation="MALINVAAREIHGKIVYYGPGLCGKTTNLQYIHGKIPSGSKGDL
                     LSIATETERTLFFDFLPLDLGKVQGFTVRFHLYTVPGQVLYERTRIAVLNGADGVVFV
                     ADAQRERLQDNLTSLKELATNISQSGKRFLDFPLVMQYNKMDLPNALPTPVLDRYLNT
                     IRVPRFEATAFNGQGVFETLRAICKLVTNKL"
     gene            complement(1184..1702)
                     /locus_tag="VHR64_03865"
     CDS             complement(1184..1702)
                     /locus_tag="VHR64_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX3301992.1"
                     /translation="MLGHFDPHLSQVIIDEETAVSINGVLRQLIRDSQATSGCVLDRA
                     GQIIVWDGPAYDGQRMQLGALIAGTYASTREVARILQEAGFRTLLQEGAREKIFTEAV
                     GDHWLVSLIFGKHTALGLVKVLCNRATMDLQQVLVNAMERQSHRPRLKDAGLDIVAND
                     TIDLIFRNETEK"
     gene            complement(1745..2815)
                     /gene="purM"
                     /locus_tag="VHR64_03870"
     CDS             complement(1745..2815)
                     /gene="purM"
                     /locus_tag="VHR64_03870"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX3301993.1"
                     /translation="MADEQAAGPISYRSAGVDIDAAATALDRVRQKIQATFPGTGAAR
                     PIGHFGGFYRLSDDEDRYLIASADGIGTKLKLAFVLGGAAHATVGADLVNHCVNDVLA
                     VGANPLFFLDYVAMGRLESDAFAGLVGGMADACRANGVAMIGGETAEMPGMYQEGEYD
                     AAGFIVGEVREAEIVDGHDVAAGDVLIGIPSGGLQTNGFSLARAILGLTGEAAHDREL
                     LDQPLALDPDTTVGNALMAPHKSFRAGVLPLARAGIVTGMAHVTGGGLIDNVPRMLPE
                     GVSAEIDPRRWEVPPIFTQLVQMGDVRQSEYHRVLNMGIGFVVAARASNAESVLAAIP
                     ESVVIGAVVPTGSDGARVSGLE"
     gene            complement(2879..3244)
                     /locus_tag="VHR64_03875"
     CDS             complement(2879..3244)
                     /locus_tag="VHR64_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX3301994.1"
                     /translation="MVDVLEEMLARLRADNDLEFAAISADGMQVASDGADSLDAEGIC
                     ATAADGYLVMTALGIELDRGDQQMLTIEYANGTVIVCPLEHGAVLVMLTGGNANLGRL
                     RLAARRFQAHYVESASAAA"
     gene            complement(3369..4742)
                     /gene="hflX"
                     /locus_tag="VHR64_03880"
     CDS             complement(3369..4742)
                     /gene="hflX"
                     /locus_tag="VHR64_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX3301995.1"
                     /translation="MAKEPEIRRHNSQFETKPESALLIAVDAHGSTWEITDSLEELAR
                     LVESVDAQVVGAVTQRLNSPVAATYVGSGKVEEIKALKESLEYDMVVANDELSPAQLR
                     NLERLLDTKVIDRTAIILDVFARRAQTHEGRLQVELAQLEYRLPRLTRMWTHLSRQAV
                     GGVGVRGPGETQLESDRREAQKRISFIKDQLNEVHRHRELHRGHRRQSETPIVALVGY
                     TNAGKSTLLNSLSGADVYAEDQLFATLDPTTRRIELNDGRTALLTDTVGFINKLPTFL
                     IAAFRATLEEIHEASVLVHVLDVTHPNAREHMATVAEVLEELGADDKPVVVALNKVDR
                     AGLGGVPTFDELMERLEIDGPSVGISAQQGTGFEALRDAIATAIDEERGVRAVQVRIP
                     YDRSEMVERFYRAASVDAVDHDETGTLLTGRIAARDLEPYREFLIHEEPAVLTAAAAT
                     GTRESAA"
     gene            complement(4743..6173)
                     /locus_tag="VHR64_03885"
     CDS             complement(4743..6173)
                     /locus_tag="VHR64_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX3301996.1"
                     /translation="MSSNPETFVEHMWPWIAHTLTGHVLAMAREGLIDQDMGETLAAA
                     IDRVREQPAADEDLVSLVRSFEQLLAAQLTPEVEAASRVGRSNVDVSATVARLATLER
                     LNALDQEARRCEAATLALAERNFVTLMPVTMDGFVSQPTSVAHWLGSLIEQLGRARDR
                     ILAALETVDASPMGANALASSGFAVDRAAIAVDLGFNRVIENTFDAVSSLDWISVAAE
                     AMDAVMNPLKRFCDEVFTWCRIEPTSFKIGDWLIDSVSGIPQWSGPFGLVRLTQDLEH
                     VAFQAHVLAHEASSVGYGPQLASLGRLAYGLDAIGTESAGLISEVAHFLEDGLLVNAA
                     YFSNRAGRSFSTSSDLADFLIIEERLTPSEAEQIAALTISRARDQGLEAAGITADLID
                     GAAMMVIGRELKVEFEAISRYLAPRRFIERRTALGAPSPDAVRTFVAHRRQDHNERNE
                     RMDSRWSHLESAALTLRSAAHEASPS"
     gene            complement(6157..6960)
                     /gene="lgt"
                     /locus_tag="VHR64_03890"
     CDS             complement(6157..6960)
                     /gene="lgt"
                     /locus_tag="VHR64_03890"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX3301997.1"
                     /translation="MPSPSSPIAFTLGPLEIRWYALFILTGIVVGTAVAAWVARRRGQ
                     NDQFLFDAAPIVVMAAVVGARLYYIALEWRYFADHPDRIIGLQLRGLTIHGALVGGIA
                     CFWWLCRRRGEPFLRWADTIMAGVPIGQAIGRWGNWANQEAFGRPTSLPWGVEIDPAR
                     RPAHYATAERFHPTFLYESICSLVIAAILVYVLLHYARRAWWRDGYALALYLVLYGVV
                     RLAIESMRTDSLYIGPWPAAYWLSGALIISGCALALYLRPREVRRVEQP"
     gene            complement(6968..>8049)
                     /locus_tag="VHR64_03895"
     CDS             complement(6968..>8049)
                     /locus_tag="VHR64_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3301998.1"
                     /translation="RPSFSDGRQLADRAVDAIGIRSGRSFSSRHTIVVDVAHIDFDET
                     AAPYVALGGCLLQLGLTTAVVVPIVLRGQVRGVLLVGFGVDAEVSPPLIAMIEDVSRR
                     AGLAIENAGLYLHARQAIEARDQFLSVAAHELRTPITSVTGYARMLLRELGDRKDPDR
                     VARYAGRLDEAGSRLATLAEDLLDVSRIRSGQLPLRVGKVDLGALAQRVALRYAEHRS
                     SARDRIVFSAADGVLPVLADPDRIEQVLTNLIDNALKYSPPDTAVELILGGDEEIVQL
                     SVRDQGIGVVPESLDAIFEPFGRAANAEETGATGLGLGLYICRTIIERLGGSIWAESE
                     GEGTGLTVTIQLPPVTKSAETARAE"
BASE COUNT         1482 a         2701 c         2477 g         1389 t
ORIGIN      
        1 gggctcgacg ttgtccgcga catcgagtgg tcataccagc gcgagacgct cgatccgacc
       61 gacagaccgt cgcccgggct ggcggagctg atcgacgagg gaaagctcgg cgaaaagtcc
      121 ggccagggct tctactctca cccgaacccc gcctacagcc agcctggctt tatcccgcag
      181 cgcaagaagg aggaatagca cggatgtatc acgtcgtctt cctggtcacc cgcaagcccg
      241 acatgtcgca ggacgagttc actgattact ggatcaatcg acacacgcca ctgaccgcca
      301 ggttacccgg cctgcgggag taccgctgct atccaatgat cgcgacgatg ggcgatcagc
      361 agccgccgtt cgatgccgtc gcctacctcg cattcgacga cgaggcatcc tgcagggccg
      421 cactcgagtc gaaggagttc acgaccgcgg tcggcgatgg cgtcaacttc cagaacgtcg
      481 acgacacgtt tggcttctac gcgcgcgagt accggatcgt ctgaacgcga agaggaccgg
      541 gcaggtgcaa gacccggtcc tcttcgcctt ggttgtctgc aattcgccgg ttagagcttg
      601 ttcgtcacca gcttgcagat ggcccgcagg gtctcgaata ccccctgccc gttgaacgcc
      661 gtcgcctcga accgtgggac gcggatcgtg ttgagatagc gatcaagaac cggggtcgga
      721 agtgcgttgg gcaaatccat cttgttgtat tgcatgacca gcgggaaatc gaggaatcgc
      781 tttccggact gtgagatatt cgtggccagc tccttgaggc tggtcagatt gtcctggagg
      841 cgctcccgct gggcatcggc gacgaagacg acgccgtccg ccccgttgag caccgcgatc
      901 ctggtccgct cgtacaacac ctgacctggg accgtgtaca ggtgaaaacg aacggtaaag
      961 ccctgaactt tgccgagatc gagcggcagg aagtcgaaaa acagggtgcg ttctgtctcc
     1021 gtcgcgatcg acaggagatc acccttgctt cccgacggga tcttgccgtg gatgtactgc
     1081 agattcgttg tcttgccgca gagaccggga ccgtagtaga cgatcttccc gtgaatctcc
     1141 cgggcggcaa cattgatgag cgccattgtt ctccctcagc ccatcacttc tcggtttcat
     1201 tccggaagat cagatcaatc gtgtcgttcg caacgatgtc gagccccgca tccttcagcc
     1261 gaggtcgatg actttggcgt tccatcgcgt tgacaagtac ctgctgcaag tccatcgtcg
     1321 cccggttgca gagaaccttg accagcccga gcgccgtgtg tttgccgaag atgagcgaca
     1381 ccagccagtg atcgcccacc gcctccgtga agatcttctc gcgcgcaccc tcctggagca
     1441 gcgtacgaaa gcccgcctcc tgcaagatac gcgccacctc tcgggtggag gcatacgtgc
     1501 cggcaatcag cgccccgagc tgcatgcgct ggccgtcgta ggccggtcca tcccaaacga
     1561 tgatctggcc ggcacggtcg agcacgcatc cgctcgtggc ctggctgtcc ctgatcaact
     1621 gccgcagcac accattgatc gagaccgctg tctcctcatc gatgatgacc tgcgacagat
     1681 gcggatcgaa atgacctagc atcgacctca ccatgcccga cgacggcagc tgcgccgccc
     1741 aggcttactc cagacctgac acacgcgcgc catctgaacc ggtcggcacg accgctccga
     1801 tgacgacgct ttccggtatt gccgcgagca ccgactctgc gttcgacgcg cgcgccgcaa
     1861 cgacgaatcc gattcccatg ttcaagactc ggtgatattc cgactggcgc acatccccca
     1921 tctgaaccag ttgcgtgaag atcggcggga cttcccacct acgcggatcg atttctgcac
     1981 taaccccttc gggcaacatc cggggtacgt tgtcgatcag gccgccaccg gtaacgtgcg
     2041 ccatcccggt cacaatgccc gcgcgcgcga gcggcagcac gccggccctg aacgacttgt
     2101 ggggcgccat gagcgcgttc ccgaccgtag tgtccgggtc gagtgcgagc ggttggtcga
     2161 gcagctcgcg gtcgtgggca gcctcgccgg tcaagccgag gatggcccgc gccaggctga
     2221 agccgttagt ttgcagcccg ccgctcggga tgccgatcag gacatcgccg gcggctacat
     2281 cgtgaccatc cacgatctcc gcctcgcgca cttcgccgac aatgaaccct gcggcgtcgt
     2341 attcgccctc ctggtacatc cccggcatct cggctgtctc gccaccgatc attgcgaccc
     2401 cgtttgcgcg gcaggcatcc gccataccgc cgaccagacc agcgaaggcg tcgctttcga
     2461 ggcgacccat cgcgacgtaa tcgagaaaga agagcggatt ggcgccgacc gcgagcacgt
     2521 cattcacgca gtggttgacg aggtccgctc cgaccgtggc atgagccgcg ccgcccagca
     2581 cgaaggcgag cttgagcttg gtgccaatcc cgtcggcgct tgcgatcagg taccgatcct
     2641 cgtcatcgga gagccgatag aaaccaccga agtggccgat tggtctggcc gcgccggtcc
     2701 ccgggaaggt cgcctggatc ttctggcgaa cgcgatcgag cgcggttgcc gccgcgtcga
     2761 tgtcaactcc ggctgatcgg taggaaatcg gcccggctgc ctgctcgtct gccacggtgg
     2821 tcccctgtct gcgtccttcg ccggatcgct ccgaaccggc acgcagaagc atgcaccact
     2881 aggcagccgc cgacgcgctc tccacgtaat gggcctggaa tcgtcgcgcc gccagccgga
     2941 gccgaccgag gttggcattg ccgccggtca gcatcaccag caccgcgcca tgctcaagcg
     3001 ggcagacgat gaccgtgccg ttcgcgtact caatggtcag catctgctgg tcgccgcggt
     3061 cgagctcgat accgagcgcg gtcatcacca ggtaaccgtc ggctgcagtg gcgcagattc
     3121 cttcggcgtc gaggctatcg gcgccgtctg acgccacctg cattccgtcg gcagagattg
     3181 cggcgaattc caggtcgtta tccgcccgga gccgagccag catctcctcg agcacatcaa
     3241 ccatgctgtc cctcgcttac gcgtgtcgtc acgcccgatt ccgagggcgc tgaagccgac
     3301 tatagcgagg caacggcagg cgcggaacgc ccgaaaccgg ccggctttgg aggttctgga
     3361 cgatagtcct aagcggcgct ttcccgggta ccggtagcgg ccgcggcggt caagacagcg
     3421 ggttcctcat ggatcaggaa ctcacgatac ggttcgagat cgcgcgccgc aattcggccg
     3481 gtcagcagtg tgccggtctc gtcgtggtcg actgcatcga ccgaggccgc gcggtagaac
     3541 cgctcgacca tctcggagcg atcgtacggg atccgaacct gcacggcgcg cacgccccgc
     3601 tcctcgtcga tggccgtcgc gatcgcatcg cggagcgcct cgaacccggt gccctgctgg
     3661 gcagagatcc cgacactcgg cccgtcgatc tcgagccgtt ccatcagctc gtcgaatgtc
     3721 gggacgccgc ccaaaccggc tcgatcgacc ttgttgagcg cgacgacaac aggtttgtcg
     3781 tcggcgccga gctcctcgag tacctccgcg accgtggcca tgtgctcgcg cgcatttgga
     3841 tgcgttacgt caagcacgtg gacgagcacc gacgcctcgt gaatctcttc gagcgtggcc
     3901 cgaaaggcgg cgatcagaaa tgtcggcaac ttgttgatga acccgaccgt atcggtcagg
     3961 agcgctgtcc gcccgtcatt gagctcgatc ctgcgagtgg tcggatcgag cgtcgcgaag
     4021 agctggtctt cggcgtagac atcggcgccg ctcagggaat tgagcagcgt gctcttgcca
     4081 gcgttggtat acccgaccag cgcgacgatc ggcgtctcgc tctgccgccg gtgcccgcga
     4141 tgcaactcgc ggtggcgatg gacctcgttg agctggtcct tgatgaacga gatccgcttc
     4201 tgcgcctccc gccggtccga ctcgagctgc gtctcgccgg gaccgcgcac gccgactccg
     4261 ccaactgcct ggcgcgacag gtgcgtccac atccgggtca ggcgcggcaa ccggtactcg
     4321 agctgggcga gttcgacctg caggcggccc tcatgcgtct gcgcgcggcg ggcgaagacg
     4381 tcgaggatga tcgccgtccg gtcgataacc ttggtatcga gcaaccgctc gaggttgcgc
     4441 aactgcgccg gcgacagctc gtcgttggca acgaccatgt cgtactcgag cgattccttg
     4501 agcgccttga tctcttcaac cttgccgctc ccgacatagg tcgcggccac cggcgagttc
     4561 agtcgctgcg tgaccgctcc gaccacctgc gcgtcgaccg actcaaccag gcgagcaagc
     4621 tcctccagcg agtcggtaat ctcccacgta ctgccatgag catcgaccgc gatcaggaga
     4681 gcggattctg gtttggtctc gaactgcgaa ttatgccggc gtatctcggg ctcctttgcg
     4741 atttaactcg gagaggcttc gtgagcggct gatcggaggg tcaaggctgc tgattcaagg
     4801 tgcgaccagc ggctgtccat gcgctcgttg cgctcattat ggtcttgccg ccgatgcgcg
     4861 acaaacgttc gcaccgcgtc aggcgacggc gcgccgagcg ctgtccggcg ctcgatgaag
     4921 cggcgcggcg caaggtaccg gctgatcgct tcgaactcga ccttgagctc gcgcccgatc
     4981 accatcatcg cagcgccgtc aatcaggtcg gcggtgatcc ctgccgcctc cagtccctgg
     5041 tcgcgggccc gcgagatcgt aagcgccgcg atctgctcag cctccgatgg cgtcaatcgc
     5101 tcttcgatga tcaggaagtc ggccaggtca ctgctggtcg agaaggaccg accggcccgg
     5161 ttcgaaaaat aggccgcgtt gacgagcaag ccgtcctcaa gaaaatgcgc gacttccgag
     5221 atcagccctg ccgactccgt cccgatcgca tcgagcccat aggcaagccg cccgagcgag
     5281 gcgagttgtg gcccgtatcc cacgctcgag gcctcgtgcg caaggacgtg cgcctggaac
     5341 gcgacgtgct ccagatcctg cgtcaagcgg acgagcccga atgggccgga ccactgcggg
     5401 ataccggaaa cgctgtcgat cagccagtcg ccgatcttga acgaggtcgg ttcgatccgg
     5461 caccaagtga agacctcgtc gcagaatcgc ttgagcggat tcatcactgc gtccatcgct
     5521 tcggcggcga ccgaaatcca gtcaagtgat gagacggcgt cgaacgtgtt ctcgatcacc
     5581 cggttgaaac cgagatcaac cgcgatggcc gcccgatcga ccgcaaaccc gctcgacgcc
     5641 agggcgtttg cgcccatcgg gctggcatcg actgtctcga gcgcggccaa aatgcgatcg
     5701 cgcgcccggc cgagctgctc gatcagggag ccgagccaat gggcgaccga tgtcggctgc
     5761 gagacgaagc cgtccatcgt caccggcatc agcgtcacga agttgcgctc ggcgagggcg
     5821 agcgtcgccg cttcgcagcg ccgtgcctcc tggtccagcg cattgagccg ctcgagcgtg
     5881 gcgaggcgcg ccaccgtggc cgaaacatcg acgttcgatc ggccaacgcg gctcgccgcc
     5941 tcgacctcgg gcgtgagctg agcggcgagg agttgctcga aggagcggac aagcgagacc
     6001 aggtcctcat cggctgccgg ctgctcgcgc acgcgatcga ttgcggccgc cagcgtctcg
     6061 cccatgtctt ggtcgatcaa tccttcgcgg gccatggcaa ggacatggcc ggtcagggtg
     6121 tgggcgatcc acggccacat gtgttcgaca aacgtctcag ggttgctcga cacgtcttac
     6181 ctctcgcggg cgcaggtaca gcgcaagcgc gcacccgctg attatcaggg ctccactcag
     6241 ccagtaagcg gccggccatg gaccgatgta cagcgaatcg gtccgcatcg attcgatcgc
     6301 cagccgcaca acaccataca ggacaaggta tagcgccagg gcatagccat cgcgccacca
     6361 tgcccgtctc gcgtagtgaa gcaacacgta aaccaggata gccgcgatca cgagcgagca
     6421 aatcgactcg tacaaaaacg tcgggtgaaa ccgctctgcc gtcgcatagt gggccgggcg
     6481 acgggccgga tcgatctcaa ccccccacgg caggctcgtc ggtcgtccga aggcctcctg
     6541 gttcgcccag ttcccccagc gcccgattgc ctgcccgatc ggcacgccgg ccataatcgt
     6601 gtcggcccag cgcagaaacg gctcaccccg ccgccggcag agccaccaga aacaggcgat
     6661 tccgccgacc agcgcgccgt ggattgtcaa tccgcgcagt tgcaggccga tgatgcggtc
     6721 cggatgatcg gcgaagtacc gccactcgag cgcgatgtag tagagccggg ccccgaccac
     6781 ggcggccatg accacgatcg gcgccgcatc gaacagaaat tgatcgttct gcccgcgccg
     6841 ccgggccacc caagccgcga ccgcggtacc gacgacgatt ccggtcaaga tgaacagggc
     6901 gtaccagcgg atctcgagcg gtccgagggt gaatgcgatt gggctggacg gtgacggcac
     6961 ccgaatccta ttccgcgcga gctgtctcgg ccgatttcgt gaccggcggc agttggatgg
     7021 tcacggtcag tccggtgcct tcgccttcgc tctccgccca gatcgaaccg ccgagccgtt
     7081 cgatgatcgt ccggcaaatg tacagcccca gaccaagtcc cgtagcgccc gtctcctcgg
     7141 cattggcagc gcgtccgaac ggctcaaaga tcgcatccag gctctccggc accacgccga
     7201 tgccctggtc acggaccgag agctgcacga tctcctcgtc gccgcccagt atcaattcga
     7261 cggccgtatc cggcgggctg tacttgagcg cattgtcgat caggttggtc aggacctgct
     7321 cgatgcggtc cggatcggcg aggaccggca gtaccccatc cgccgcggag aagacgatcc
     7381 ggtcccgtgc cgacgaccga tgctcggcat accgaagcgc gacacgttgc gcaagcgccc
     7441 cgagatcgac ctttccgacc cgcagcggca actgtcccga tcggatccgt gagacgtcga
     7501 gcaaatcctc agcgagcgtg gccaatcggc tccccgcctc gtcgagtcga ccggcatagc
     7561 gggcaacgcg atctggatcc ttccggtcac cgagctcacg caggagcatc cgggcgtagc
     7621 cggtaaccga cgtgatcggc gttctcagct cgtgcgcggc aaccgacagg aactggtcgc
     7681 gcgcttcgat cgcctgccgg gcgtgcaggt agagcccggc gttctcgatc gcaagcccgg
     7741 cacgacgcga cacgtcctcg atcatcgcga tcagcggagg agagacctcc gcatccacac
     7801 cgaagccgac cagaaggacg ccacgcacct ggccgcgcag gacgatcggg accacgaccg
     7861 cggtcgtcag cccaagctgc aacaggcagc caccgagggc aacgtacgga gccgccgttt
     7921 cgtcgaaatc gatgtgcgcg acatcaacga cgatcgtgtg ccggctgctg aacgatcgtc
     7981 cggatcggat gccaatcgcg tcgacggcgc ggtcggcaag ctgccgtcca tcgctgaagg
     8041 acgggcgat
//
LOCUS       DATAOZ010000141        15025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4792717, whole genome shotgun sequence.
ACCESSION   DATAOZ010000141 DATAOZ010000000
VERSION     DATAOZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15025
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4792717"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="pip"
                     /locus_tag="VHR64_03900"
     CDS             <1..1018
                     /gene="pip"
                     /locus_tag="VHR64_03900"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX3301999.1"
                     /translation="PIRSAIIRAHVINREQAGVTFPPIEPFATGHLPVGDGDEIYWEA
                     SGNRDGKPALYLHGGPGSGVGTGYRRNFDPDQYLIVSFDQRGCGRSRPLVSDPTADLS
                     TNTTQALISDIEALRNHFGVERWLIMGLSWGTTLALAYAQAHPDRVSELVLGAVTTTS
                     PEEVEWITESVGRIFPEEWDRFAAASGRKPGQRVIDAYSELITSPDPAVREAAARDWC
                     RWEDVHVSLDPNHAPHPGFDDPEFRMVFATLVIHYWKHAGFAGEGGLLAGMDRIAHIP
                     GVLAHGRIDVSGPLVTAWELHKRWPGSELIVIEHEGHGGDAMIDALRRAIAHFAPNES
                     ATIP"
     gene            1015..1827
                     /locus_tag="VHR64_03905"
     CDS             1015..1827
                     /locus_tag="VHR64_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX3302000.1"
                     /translation="MIFDELLRRWDLTPDGDPIVTHSSQLLPVCRGDQPAMLKVATEP
                     EETRGGAVMVWWEGEGAARVLAHHGAALLLERATGTRSLIEMVRAGQDDAASRIICGV
                     VARLHAPRDSLPPSLVPLAEWFRELWPAAEQYSGILRRSAETARLLLADQREVVVLHG
                     DIHHGNILDFGPHGWLAIDPKGLIGERGFDFANTFCNPHQEMITVRERVSRTADVISA
                     AAQFDRTRLLQWVLAYAGLSAAWFLGGAVGDPASAQQPIAIAELAAEELDQN"
     gene            1907..2368
                     /locus_tag="VHR64_03910"
     CDS             1907..2368
                     /locus_tag="VHR64_03910"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3302001.1"
                     /translation="MNVIDIRGFTEDAIVGPETGATRLFIWCTTVQAGHVVGLHKHLG
                     EELSRVLYGRLRFRAGDQVQEIGAGEVVIVEPGTLHGYIALEDTEIEIYGEIGSGIFV
                     PANGSNGSTDFEEIYVRDVPWSRAPREERQYITRAEQLERFRSRYAADPFV"
     gene            complement(2344..2772)
                     /locus_tag="VHR64_03915"
     CDS             complement(2344..2772)
                     /locus_tag="VHR64_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302002.1"
                     /translation="MNVSLGPRLHRIDRDRIAPLVVGGIWLAGAAFNAISTLRQPDPY
                     TWLEESKVQPYRWFFGEVAGRNPSFWTLALIAGEIALGLLTIAGGKWTRLGLLGGTLF
                     SLFLFSLATPYTMMMGGYAALLAWLAWRERRSDQTKGSAA"
     gene            complement(2932..4485)
                     /gene="pckA"
                     /locus_tag="VHR64_03920"
     CDS             complement(2932..4485)
                     /gene="pckA"
                     /locus_tag="VHR64_03920"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX3302003.1"
                     /translation="MAATTRPRVHARPSIAPTAPEPSADSHRYSYPSVPFLYEQSLER
                     NESRLAENGPLAVETGIHTGRSARDKFVIEDSITRESVWWGKVNQRMTPEHFAALQRD
                     VIAHLEERPTVTIDLRAGADLRFAIPVQLTTEHAWAALFARNLFLPPDDGAALEPFRI
                     LHAPTFDADPATHGTRSSTVIAISFSERTVIVAGTHYAGEIKKSIFSVLQFLLPEFGV
                     ATMHCAANMGEKGDVALFFGLSGTGKTTLSTDKDRILVGDDEHGWSDDGVFNFEGGSY
                     AKVIKLSPKAEPGIWNASHRFGTVLENVVMDAETRALDLDDASLTENTRSAFPLSYIP
                     GASETGQGGHPRHIIFLTADAFGVLPPVAKLTPEQAHDWYLAGYTSKLAGTEKGVTEP
                     AATFSACFGDAFLTHPPQRYAELLDARIAAHNPSLWLVNTGWTGGAYGTGERISIAHT
                     RAIIRAILAGELEDVQLETEPIFGLHLPTRVKDVPREILRPWHGWSNYGAYENAASRL
                     AADFRNAIR"
     gene            complement(4655..5680)
                     /locus_tag="VHR64_03925"
     CDS             complement(4655..5680)
                     /locus_tag="VHR64_03925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3302004.1"
                     /translation="MTTPIPLSFLDLTPIENGSNATDALATSVELAKLAEELGYVRIW
                     YAEHHNTGGLASAAPEIMIAHVATQTERIRLGSGGVMLPNHAPLKIAETFTLLEALHP
                     GRIDLGLGRAPGTDTITAFAMRRSREALTADDYPQQVAELIAFDDESFPAGHPFGMIR
                     VVPFDVRMPPLWMLGSSEFSGHLAAQLGLGFGFAAHISRALAIPTMRAYREHFVPSAR
                     YPEPHGILTVSVTIGETPEHAADLSLINELFLLRLRTGQLGRYPTLEEAKTYQFTEQE
                     RALIASMPLNFIAGTADQVFGEVMDMAALCQADEVMITTTLPSPEDRRRTLTSMAEQF
                     GLVAAPA"
     gene            complement(5864..6505)
                     /locus_tag="VHR64_03930"
     CDS             complement(5864..6505)
                     /locus_tag="VHR64_03930"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX3302005.1"
                     /translation="MRALFTREDNGGRETARIEAFSDGVIAIAITLLVLDVKVPPHDQ
                     VSDLWRSLGELWPNYVGFALSFVIIGIIWANHHDMFGLIGRVDRTLVLINLLFLLCVG
                     FLPFPTALLAEYLGHDGERAAVNVYSGWMLATALVYCLLWWYPRRAGLIDPAASPQAV
                     ADLVRSFRIGPPSYVLAFLLSFVSTWASLVLLALLALAYLIPPSWLEMHRSQS"
     gene            6673..6891
                     /locus_tag="VHR64_03935"
     CDS             6673..6891
                     /locus_tag="VHR64_03935"
                     /inference="COORDINATES: protein motif:HMM:NF022716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEX3302006.1"
                     /translation="MTDQNLPEQSQNPVRDPEDWVTGDEPMTGPQASYLRTLLQQAGK
                     EDDFDPKLTKANASKLIDKLQDKTGRGN"
     gene            6969..8312
                     /locus_tag="VHR64_03940"
     CDS             6969..8312
                     /locus_tag="VHR64_03940"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HEX3302007.1"
                     /translation="MATSAAVTGFDSAWSRKNRGAVAHIAAGNGAMQFIEPRTASFQE
                     ALASINEVGKNANVHIVGIDQPLIVPNQAGCRPVERAFQPLLSSVRGAIQPANRGKSD
                     LFGDDAPIWTFLGALNADVDWSHAIGAASGRYAIEIYPAAALLGLLPHFLGRKLAAKY
                     NPTNQTFNLADWRLVCDSLASLAAELEIVGLPDWASAHGQISKPRKPDQDCLDAAICA
                     VVTYLWWRYGFERSIVVGDLRTGYIVTPSNPAMTKRLADRAVEPSVPVHVTGLARDTT
                     AVNRESLGVNIREAVPDDAEAIAAIYNQGIEDRVATLETQLRTADERAQWLAAKGPRH
                     PVLVALDGEGSVVGWGSLNQFNPRPAYDHVVEFSVYVGREQRGQGIGSVLLSALEDRA
                     RSLGFHKMVLAAFPWNAGGMRLYQKHGFATVGTFHEQGRLDDRWVDVILMEKILS"
     gene            complement(8322..8681)
                     /locus_tag="VHR64_03945"
     CDS             complement(8322..8681)
                     /locus_tag="VHR64_03945"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX3302008.1"
                     /translation="MRRDGNIVSYTADELDEMLARGEDLTDWAKVDALTDEELEASID
                     VEEEGEFDWENAIPVSIILDPKRQITIRLDQDVLDWFKAQGSGYQTRINAVLRSYVDA
                     ERKAEDERARPIRRKAS"
     gene            complement(8644..8916)
                     /locus_tag="VHR64_03950"
     CDS             complement(8644..8916)
                     /locus_tag="VHR64_03950"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX3302009.1"
                     /translation="MIEFEWDEAKRLANIEKHDIDFRRARLLFDGRTILITLVPKPVE
                     IRLSAIGELDGRMVTAYWTIRGRMIRLISVRRARREERRKYRQLYG"
     gene            complement(8947..9912)
                     /locus_tag="VHR64_03955"
     CDS             complement(8947..9912)
                     /locus_tag="VHR64_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3302010.1"
                     /translation="MSDRDPGIYAQETAVTEIGEGLWQIDLGFQDRAGVIAAYLMAGE
                     GELALIETGPSSCLTNLRAGIAQTGHDISELTHALVTHIHLDHAGAAGPLARDNPNLR
                     VSVHPFGAPHLIDPSKLVASATRIYGDQMEPLWGEFAPIAEEQVHVLLDGEVIKVAGR
                     RILAAFTPGHAHHHAAFVDLADRIAFTGDVGGVRMQGTDYVCPPTPPPDLDPVKWWES
                     IDRLQGFDLDRLYLTHFGEFRDVDRHLADLGPNLDEFLHIGTDALDAGAEPDELTRRL
                     HERMERGVGDVHPGVLVNLEWATPSYMAALGLIRWHKKQREARGT"
     gene            complement(10044..11441)
                     /locus_tag="VHR64_03960"
     CDS             complement(10044..11441)
                     /locus_tag="VHR64_03960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3302011.1"
                     /translation="MWSPQTSLVDAEPAVYWTSQPRPVPIQPPLRTNATADLVIIGGG
                     FTGLWAAIEAKQRDPNRDVVLLEMERIAFGATGRNGGFLEPSLTHGLENGLARYPADE
                     MRTLLRLGNENYAEIADFIHAHQIDCDLQEHGVIWAATAPYLSEAIPKIVTLLREWGQ
                     DTVALSAEELRAEVDSPFYLGGVWSRGEGGLVDPARLAWELLRVALELGVRVHERTRV
                     TGLVDAGARMVVRTAGASILAGKVLHATSAYPGVIPETRRYVVPVYDYVLMSEPLTEA
                     QRAAIGWRTRFGLSDGDNQFVYHRMTADHRILYGGWDAVFHRKGVDPSFDQRDASHQF
                     LAERFFRTFPQLEGLRFTHRWGGAIDTCSRFAVFFNTTHGGKVAYAAGYTGLGVGASR
                     FGARTALDLLDGQSTERTELRLTRKRPVPFPPEPVRSAVIGFTQREIARADRSQGQRG
                     LWLRTLDRLGLGFDS"
     gene            11672..12466
                     /locus_tag="VHR64_03965"
     CDS             11672..12466
                     /locus_tag="VHR64_03965"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC and DUF2382 domain-containing protein"
                     /protein_id="HEX3302012.1"
                     /translation="METPMTQYQFRAGDDVIAADGDKIGNLADLHGTYLVVEKGFFFP
                     RDYYIPFSTVANYDPGDGKIYLSVTKDAALNSGWDHKPDLSAEDRAYAAGAPRDTYAT
                     DTTATTTGRTRDTYATDRTTEVTDQDHISVPVHEEELIPTKTVRQAGQVRVDKDVIEE
                     EREIDVPVTEERVKVTRRAVDQVADTGDMAFREESFEVPVRTEDVGVDKVVRVAEQVD
                     IDKQAIQDTKRVSGTVRKERVDVDEAGTDASIYADTNRVTDDTAST"
     gene            12577..13116
                     /locus_tag="VHR64_03970"
     CDS             12577..13116
                     /locus_tag="VHR64_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3302013.1"
                     /translation="MTKPVFETERLIVREYTVDDAPAMFDIYKDPEVWRYLAGGDPYT
                     SVGQARERIELRIQRYREGAPYGLWAVVVRDTAQLIGSVLLSPLEDGPDVEVGYHFGR
                     FAWGNGYATEVTRGAIRYGFDVLGLPSICGVVFQENAASQRVLEKAGMTYRGLRSVFG
                     FDGVMYYTIESPTGCDQPG"
     gene            13214..14101
                     /locus_tag="VHR64_03975"
     CDS             13214..14101
                     /locus_tag="VHR64_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302014.1"
                     /translation="MNRRAFLAGSTGLACLATLGPRTASALAHAASTPVAEDTFPTLA
                     IGLKDDGFELPDAIQAGRYHVTVTNHGTSDASHSALGRIPDQVTDAQYQEWLDSLSRT
                     DGTDGQTDALTWDDIEFVGMPDWPKAGQSVSGVIDLAPGRYFLFDPFSSRGDMSLTIT
                     GDPVSAPEPESDLTVTLVDMKINLPDAAITSKPVRWKIENTGSISHEVAVIPVTPTFT
                     EEDLTVLLSLPEDGTPPAGTDNLDYQPVAAIGILAKGHTSWLDVKLTPGHYLAACMLP
                     FGTGYPHAMDGMYRFFEVK"
     gene            14217..>15025
                     /locus_tag="VHR64_03980"
     CDS             14217..>15025
                     /locus_tag="VHR64_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302015.1"
                     /translation="MTQTPGLRARFIRDWPAAAWVASAIVINIAGARSAPQSWHQMVA
                     LTDIPASDRAELRRSLADLHLSPVFLACFQIATAVIGTIVNLIIGWLLIRRAPRTGFA
                     IYLAFIMLAMTNANYPPSIADMLPGQPVAQFVIRLSTAVAISGFFTLPFLFPDGRFVP
                     RWTVLCWIYVVIGVFTFAFFPSYKPGGSTWDIANAIGTVLLILSLLFAVIYRYRRVST
                     PLQKRQTKWVAFGLGVSVPAFFAADAMMRNIDATPAGIGCLLAFLVIMPIAT"
BASE COUNT         2766 a         4942 c         4653 g         2664 t
ORIGIN      
        1 gcccatccgc tcggcaataa tccgggcgca cgtgatcaat cgggagcagg caggagtgac
       61 gtttccacca attgaaccct ttgccaccgg tcaccttccg gttggtgacg gggatgagat
      121 ctactgggag gcgtccggga accgcgacgg taagccggcg ctctacctgc acggcggacc
      181 tggtagcggc gtcgggaccg gctaccggcg aaacttcgac cccgaccagt acctgatcgt
      241 ttccttcgac cagcgcggct gcggccggag tcgtccgctg gtcagcgatc ccacggctga
      301 cctctccacc aacacgaccc aggcgctgat ctcggacatc gaggcgctcc ggaatcattt
      361 cggcgtcgag aggtggctca tcatggggct ctcgtggggc acgactctcg ccctggccta
      421 cgcgcaagcc caccctgacc gggtctccga gctggtcctc ggcgcggtca cgacgacgag
      481 cccggaggag gtggagtgga tcaccgaatc ggtcggccgc attttcccgg aggagtggga
      541 ccggttcgcg gccgcatcag gtcggaagcc ggggcaacgg gtgatcgatg cctactccga
      601 gctgatcacg agtcccgatc cggccgttcg cgaggctgcc gcgcgggatt ggtgccggtg
      661 ggaggatgtc cacgtctcgc tcgaccccaa ccacgcgcct catcccggct tcgatgaccc
      721 cgaattccgg atggtctttg cgacgctggt catccattac tggaagcacg ccgggttcgc
      781 cggagagggc ggtctgctcg ccgggatgga ccggattgct catatccccg gcgttctcgc
      841 ccacggccgg atcgacgtca gcggccccct ggtcaccgct tgggagctgc acaagcgctg
      901 gccgggcagc gagctgatcg tgatcgagca cgagggtcat ggcggagacg cgatgatcga
      961 tgcgctgcga cgggccattg cccatttcgc accgaacgaa agcgccacga taccgtgatc
     1021 ttcgacgaat tgctcagacg ctgggacctg accccggacg gcgacccgat cgtcacacac
     1081 tcgagccaat tgctaccggt ctgccgtggc gaccagccgg cgatgctcaa agtcgcgacc
     1141 gagcctgaag agacgcgtgg cggcgcagtg atggtgtggt gggaaggcga aggcgccgct
     1201 cgcgtcctcg cccaccatgg cgctgcgttg ctccttgagc gcgccaccgg aacccgctcg
     1261 cttatcgaga tggttcgcgc tggtcaggac gacgcggcca gccggatcat ctgcggcgta
     1321 gtcgcccggt tgcatgctcc gcgtgactca ttaccgccca gcctggtccc gctggccgaa
     1381 tggttccgtg aactctggcc ggcggctgag cagtacagcg gcatcctgcg ccgctctgcc
     1441 gagactgccc ggttgctcct tgccgaccag cgtgaggtcg tggtgctgca tggcgacatc
     1501 caccacggca acatcctcga ctttggtccg cacggatggc tcgccatcga tcccaaggga
     1561 ctcatcggcg agcgcggatt cgatttcgcc aacaccttct gcaacccgca ccaggagatg
     1621 atcacggtgc gggaacgggt ttcccgcacg gccgacgtca tctccgccgc agcccaattc
     1681 gaccggaccc gactactgca atgggttctt gcctatgccg gtctctcggc ggcctggttc
     1741 ctcggcggcg cagttggcga tccagcgtcg gcacagcagc ccatcgcgat cgccgagctg
     1801 gccgctgagg aattggacca aaactgaccc gggttaccta tcgaccggcg atccagatgg
     1861 gtatatcctt gacccctcaa acagtaaata cctgaaaggc cccgcgatga acgtcatcga
     1921 tatccgaggc tttaccgaag acgcgatcgt cggtcccgaa accggcgcga cccggctttt
     1981 catctggtgc accactgtcc aggcgggaca tgtcgtcggg ctgcacaagc acctcggcga
     2041 agagctctcc cgcgtccttt acgggcggct tcgcttccgc gccggcgacc aggtccagga
     2101 gatcggcgcc ggcgaagtcg tgatcgtcga acccggtacc ctccacgggt acatcgcgct
     2161 cgaggacacc gagatcgaga tctacggcga gatcggctcc ggcatcttcg tcccggccaa
     2221 tggttccaac ggcagcacgg acttcgagga gatctacgtg cgggatgtgc catggagccg
     2281 cgcaccgcgg gaggagcgtc agtacattac ccgggccgaa cagctcgaac gcttccggtc
     2341 acgctacgcc gccgacccct tcgtctgatc cgatctgcgt tctcgccacg ccagccaggc
     2401 cagcaatgcg gcgtacccgc ccatcatcat cgtgtagggc gtcgccaggg agaaaaggaa
     2461 caagctgaag agcgtgccgc ccagcagccc caggcgcgtc cacttgccgc cggcaatcgt
     2521 caggagacca agcgcgatct ccccggcgat gagcgccagc gtccaaaacg acggattcct
     2581 gcccgcgacc tcgccgaaga accagcgata aggctgcacc ttcgactcct cgagccacgt
     2641 atagggatcc ggctgccgca acgtcgagat ggcattgaat gccgccccgg ccagccagat
     2701 cccgccaacg accagcggcg caatccggtc acgatcgatc ctgtgcaacc tcgggccgag
     2761 tgagacgttc atggcgttcc tccgtgtctc gtcagcccga tcgtgcccaa cgtggcattg
     2821 cgatcattac ggcctttttc gattggacgt tgcgaaatcg ccacaaacac gcggaccccg
     2881 gagcgccgtc tctggcagct ccggggtccg gtcttgtagc gagtgatctg cttaccgaat
     2941 cgcgttccgg aagtcggccg ccagtcggct ggccgcgttc tcgtaggcgc cgtagttgga
     3001 ccacccatgc cagggccgaa ggatctcccg cgggacgtct ttgacccgcg tgggcaggtg
     3061 aagaccgaag attggctcgg tctccagctg cacgtcttcc agctcgccgg cgaggatcgc
     3121 gcggatgatc gcgcgggtgt gggcgatcga gattcgctcg cccgtgccgt acgcacctcc
     3181 ggtccagccg gtgttcacca gccagagcga tgggttgtgc gcggcgatcc tggcatccag
     3241 taattccgcg tagcgctgcg gtgggtgcgt caggaaggca tccccgaagc acgcggagaa
     3301 ggtcgcggcc ggctcggtca cccctttttc ggtgccggcc agcttggacg tatatccggc
     3361 caggtaccag tcgtgcgcct gctccggcgt cagtttcgca accggcggga ggacgccgaa
     3421 ggcatcggcc gtcagaaaga tgatgtgacg cgggtgcccg ccttgcccgg tctcggacgc
     3481 gcccgggatg tagctgagcg ggaaggccga gcgcgtgttc tcggtgagcg aggcgtcgtc
     3541 gagatcgagc gcgcgcgtct cggcatccat gaccacattc tcgagcacgg tcccgaagcg
     3601 atggctggcg ttccagattc ccggctccgc ctttggcgag agcttgatca ccttggcata
     3661 ggagccgccc tcgaagttga agacgccatc gtccgaccag ccgtgctcgt cgtcaccgac
     3721 gaggatccgg tccttgtcgg tcgacagcgt ggtcttgccg gtgccggata gcccgaagaa
     3781 gagcgcgaca tcgccctttt cgcccatatt ggcggcgcag tgcatggtgg cgacgccgaa
     3841 ctcgggcagc aggaactgaa gtaccgagaa gatcgacttc ttgatctcgc ccgcgtagtg
     3901 cgtcccggca acgatcacgg tccgctccga gaacgaaatt gcgatcaccg tgctcgagcg
     3961 ggtgccgtgc gtggccggat cggcatcgaa ggtcggggca tgcaggatcc ggaagggctc
     4021 aagcgccgcg ccgtcgtccg gtggcaggaa caggttgcgg gcgaagaggg ctgcccaggc
     4081 atgctcggtc gtcaactgga ccgggatcgc aaagcgaaga tcggccccgg cccgaagatc
     4141 gatcgtgacg gtgggccgct cttcgaggtg ggcgatcacg tccctctgca gcgccgcgaa
     4201 atgctccggc gtcatgcgct ggttgacctt cccccaccag accgattcgc gagtgatgct
     4261 gtcctcgatg acgaatttgt ccctggcgga gcggccggta tggatgccgg tctcgacagc
     4321 cagcgggcca ttctcggcca gacgcgactc gttccgctcg agtgattgct catacaggaa
     4381 cggcactgaa ggataggaat accggtgact atccgcgctg ggctctgggg cagtcggcgc
     4441 gatcgaaggg cgcgcatgca cgcggggcct ggtcgtagca gccacggtga aacctcctta
     4501 caggcctgaa agtctgtaac ggtgtagata ttgcgatgaa aatcactatc aacaccgaga
     4561 gaaaccgagt atacacccgc cattttgcac tgtcagtagc cgtccgccgg gacgcaggtc
     4621 ccgattttgg cgtgtccacc gcaacccctc tgcgtcaggc tggagcagcc accaaaccga
     4681 actgttcggc catactcgtc agggtccggc ggcgatcttc ggggctcggc aacgtcgtcg
     4741 taatcatcac ctcgtccgcc tggcagagcg cggccatgtc catgacctcg ccgaagacct
     4801 gatcggccgt gccggcgatg aagttgagcg gcatcgaggc gatcaatgcg cgctcctgct
     4861 ctgtaaattg atacgttttc gcctcctcca gggttgggta tcggccgagc tggccggttc
     4921 gcaagcgcag caggaacaac tcgttgatca gggagaggtc ggccgcgtgc tccggcgtct
     4981 ccccgatcgt cactgacacg gtcaggatcc cgtgcggctc cgggtaccgg gccgacggca
     5041 cgaagtgctc gcggtacgcc cgcatcgtcg ggatcgccag cgcacgactg atgtgcgccg
     5101 caaacccgaa gcccagcccc aactgcgccg ccaggtgacc gctgaactcg ctcgacccca
     5161 gcatccacag cggcggcatc cggacatcga acggcacgac ccggatcatc ccgaaggggt
     5221 gaccggccgg gaacgactcg tcgtcgaacg cgatcagctc agcgacctgc tgcgggtagt
     5281 cgtcggcggt cagcgcctcc cgggagcggc gcatcgcgaa ggccgtgatc gtgtccgtgc
     5341 ccggcgcccg gccgagtccg agatcgatcc gacccgggtg gagcgcctcg agcagggtga
     5401 aggtctcggc gatcttgagg ggcgcgtggt tcggcagcat cacgccgccc gagccaagcc
     5461 ggatccgctc ggtctgggtg gcgacgtggg caatcatgat ctcgggtgcg gcgctggcca
     5521 atccgcccgt gttgtggtgc tcggcatacc aaatgcggac ataaccgagt tcctctgcca
     5581 gcttcgcgag ctcaaccgac gtcgccagag catcggtcgc attcgatccg ttctcgatcg
     5641 gcgtcaggtc gaggaacgaa aggggtatcg gggttgtcat caataactcc ggggtagtcg
     5701 gtgcgatcag gcaagccgat cgcttgtctt aggtaagcaa ctgtaccgga gtggtcaaat
     5761 cgtgccggga ccaacctcga acgtagtgcc gggcgaccca ccagacttcc catcgctcaa
     5821 caaccgagcg agccgctcgg cactacgttt ggaaagccgt gaatcaggac tgtgaccggt
     5881 gcatctcgag ccagctcggc gggatcaggt aggcaagtgc cagcaacgcg agcagcacca
     5941 ggctcgccca ggtcgagacg aacgagagca gaaacgccag gacatagctg ggtggaccaa
     6001 tccggaagct acgaacgaga tcggccaccg cctgcgggct tgcggcagga tcgatcagac
     6061 cggcgcgacg cgggtaccac cagagcaggc agtagacgag cgccgtggcc agcatccagc
     6121 ccgagtacac attgactgcc gcccgctccc cgtcatggcc caggtactcg gcgagcagcg
     6181 ccgtcgggaa gggcaggaag ccgacgcaga gcaggaaaag caggttgatc agcaccaacg
     6241 tccgatccac ccggccgatc agaccgaaca tgtcgtggtg gttcgcccag atgatgccga
     6301 tgatcacgaa gctgagcgcg aagccgacgt agttcggcca gagttcgccg aggctgcgcc
     6361 agagatcgct gacctgatcg tgcggcggga ccttgacatc gagcacgagc agcgtgatgg
     6421 cgatcgcgat tacgccgtcg ctgaacgcct cgatccgcgc cgtctcgcgc cctccattgt
     6481 cttcccgcgt gaacagcgcc cgcattcccg ctccccgcac gtcccgagcc gtgtcgctcc
     6541 agcataccgg gacaccagcg aagcgcgacc tgcccgttcc gtgaggacgt cgggttagcc
     6601 gggctacaag cgacggcttg cacgtcagta cgcatgttct attatgtatg tcttgaacga
     6661 gaggagacgc agatgaccga ccagaacctt cccgagcaga gccagaaccc ggtgcgcgat
     6721 cccgaggact gggtaaccgg cgacgagccg atgaccgggc cacaagcctc ctacctccgc
     6781 accctgctgc agcaagccgg caaggaggac gacttcgacc cgaaactcac caaggccaac
     6841 gcctcaaagc tgatcgacaa gctccaggac aaaaccggcc gcggcaacta gctgtttctg
     6901 atccaggtcc cgcgccgtat ccttttgttt atcgatattc cgtcgatgaa gaagggcgcg
     6961 ggaccacgat ggcaaccagt gcagcggtta ccggattcga ctccgcgtgg agccgaaaga
     7021 accgcggagc cgttgcccat attgccgcgg gcaacggcgc tatgcaattc atcgaaccga
     7081 gaaccgcctc attccaagag gctctggctt ctatcaatga ggttggcaaa aacgccaacg
     7141 ttcacatcgt gggcatcgac cagccattga tcgttccgaa ccaggccggg tgtcgccccg
     7201 tcgagcgagc gttccaaccg ctcttatcct ctgtccgtgg ggcaatccaa cccgccaacc
     7261 gcgggaaatc ggacctgttt ggagacgacg ctccgatctg gactttcctt ggcgcgctga
     7321 acgcggatgt cgactggtcg cacgccatcg gagcagcatc cggtcgatac gcgatcgaga
     7381 tctatcctgc tgccgctctg ctcggactat tgccccactt tctcgggagg aagctggcgg
     7441 ccaaatacaa ccccacaaac caaacgttca acttggccga ctggcggctc gtctgtgatt
     7501 cccttgcgtc cctggccgcg gagctcgaaa tcgtgggctt gccagattgg gcttcggccc
     7561 acgggcaaat cagcaaaccg cgcaagccgg accaggattg ccttgacgcg gcaatctgtg
     7621 cggtcgtgac gtacctctgg tggcgatatg gatttgaacg ctcgatcgta gttggcgatc
     7681 tccggactgg atacatcgtc acaccgtcta acccggcaat gacgaaacgg ctggcagatc
     7741 gtgccgtcga accatcggtt cctgttcatg tcacaggatt ggcgcgagat acgacagcgg
     7801 tgaaccggga aagtctcgga gtcaacatcc gggaggcagt gccggacgac gcggaggcga
     7861 ttgcggcgat ttacaaccag gggatcgagg atcgggttgc gacgctcgag acccagctcc
     7921 ggaccgccga cgagcgggcg cagtggctgg cggcgaaggg accgcgccac ccggtgctgg
     7981 tcgcgctcga cggcgaggga tcggtggtgg gctgggggtc gctcaatcaa ttcaacccac
     8041 gcccggccta cgatcacgtc gtcgagttct cggtctatgt ggggcgggaa cagcgtggcc
     8101 aggggatcgg aagcgttttg ctgagcgcgc ttgaggatcg ggcgcgctca cttggattcc
     8161 acaagatggt gctagcggcg ttcccgtgga atgcgggcgg catgcgcctc taccaaaagc
     8221 acggcttcgc gacagtcggc accttccacg agcagggccg cctcgacgat cgctgggtcg
     8281 atgtgatcct gatggagaag atcctcagtt gagcgactag gtcacgaggc cttccggcga
     8341 ataggtcgtg ctcgctcgtc ttccgccttc cgttctgcgt caacgtagct gcggagcacg
     8401 gcattgatcc gggtctggta accgctgcct tgcgccttga accagtccag cacgtcctga
     8461 tcaagcctga tcgttatctg ccgcttggga tcgagaatga tcgaaaccgg gatcgcattc
     8521 tcccagtcga attcaccctc ctcctcaacg tcgatcgacg cctcaagttc ctcatcggtc
     8581 aacgcgtcca ccttggccca atcggtcagg tcctcgcctc gcgcgagcat ttcatcaagc
     8641 tcgtcagccg tatagctgac gatatttccg tctctcctca cgtcgcgccc tccgtactga
     8701 aatcagccgg atcatccggc cgcgaatcgt ccaataggcg gtgaccatcc tgccatccag
     8761 ttctccaatc gcggagagtc tgatctcgac gggttttggg accaacgtga tgagaatcgt
     8821 gcgaccgtca aacaacagtc gcgcacggcg aaagtcgatg tcgtgtttct cgatatttgc
     8881 gaggcgcttc gcttcgtccc attcgaactc aatcacgacg tgtaactaca agtgtaacta
     8941 cagcggtcaa gtcccccgcg cctcccgctg cttcttgtgc cagcggatca ggccaagcgc
     9001 ggccatatag ctcggcgtcg cccactcgag gttgaccagc acaccgggat gcacgtcacc
     9061 cacgccccgc tccatccgct cgtgcaagcg ccgggtcaac tcgtccggct cggcgccggc
     9121 atcgagggcg tcggtcccga tatgcaggaa ctcgtcgagg ttcggaccga gatccgccag
     9181 gtgtcgatcg acatcccgaa actcgccgaa gtgcgtcagg tagagccggt cgaggtcgaa
     9241 tccctgcaac cggtcgatgc tctcccacca cttgacagga tcgagatccg gcggcggagt
     9301 cggcgggcag acgtagtcgg taccctgcat gcgcacgccg ccgacatccc cggtgaaggc
     9361 gatccgatcg gccaaatcga cgaaggcggc atggtgatgg gcatgtcccg gcgtgaaggc
     9421 cgccaggatg cgccgacccg ccaccttgat cacctcgccg tcgagcagta cgtgaacctg
     9481 ttcctcggcg atcggcgcga actcccccca tagcggttcc atctggtcgc cgtagatccg
     9541 ggtcgcgctg gcgacgagct ttgagggatc gatcaagtgc ggcgcgccga agggatgcac
     9601 ggagacacgc aggttcgggt tgtcgcgcgc cagtggtccg gccgcgccgg cgtggtcgag
     9661 atggatgtgc gtgaccagcg catgagtcag ctcgctgatg tcgtggccag tttgggcgat
     9721 tcccgcccgc agattggtca ggcatgagct cggcccggtc tcgatcagcg ccagctcacc
     9781 ttcgccagcc atcaggtaag cggcaatcac cccggcccgg tcctgaaacc cgaggtcgat
     9841 ctgccagagc ccctccccga tctcggttac ggccgtctcc tgcgcataga taccggggtc
     9901 gcgatcactc atggctttcc tcagtggtca gtggtcagtg gtcagtggtc agtagtcagt
     9961 agtcagtggt cagtggtcag tggtcagtgg tcagtggtca gtgagagcgt agccccaact
    10021 gctcgcctgc tcacctgctc gcctcatgaa tcaaatccca accccagccg atcgagtgtc
    10081 cgcagccaga gaccgcgttg gccctgactg cgatcagcgc gggcgatctc tcgctgggta
    10141 aagccgatca ccgccgagcg gaccggctcc ggcgggaagg gcaccggccg ctttcgggtc
    10201 aagcgcagtt cggtccgctc agttgactgg ccatcgagca ggtccagcgc cgtccgggca
    10261 ccgaaccgcg acgcgccgac gccgagtccc gtatatcccg ccgcgtacgc gaccttgccg
    10321 ccgtgcgtcg tgttgaaaaa gacggcgaag cggctgcagg tatcgatcgc cccgccccac
    10381 cggtgcgtga agcgcagtcc ctcaagctgt gggaaggtcc gaaagaaccg ctcggccagg
    10441 aactgatgcg atgcatcgcg ctgatcaaac gacgggtcga cgccctttcg gtgaaagacc
    10501 gcatcccatc cgccgtagag gatccggtgg tcggcggtca tgcggtggta gacaaactgg
    10561 ttgtcgccat cgctcaaccc gaaccgggtc cgccagccga tcgccgcgcg ttgcgcctcg
    10621 gtcagcggct cgctcatcag cacgtagtcg tagaccggga ccacgtaccg cctggtctcg
    10681 ggaatgacgc ccgggtaggc actggtcgca tgcaagacct tgccggccag gatcgaggca
    10741 cccgctgttc gcacaaccat tcgcgcgccg gcatcgacca gcccggtcac acgtgtcctt
    10801 tcgtgaaccc ggacaccgag ctcaagcgcc acgcgcagca attcccaggc caaccgggcc
    10861 ggatcaacga ggccgccctc gccccgcgac cagaccccgc ccaggtaaaa gggtgaatcg
    10921 acctctgccc gcagctcctc agccgacaag gcgacggtgt cctggcccca ctcccggagc
    10981 agggtcacga tcttcgggat cgcctcggac aggtagggcg cggtcgccgc ccagatcacg
    11041 ccatgctcct gcaggtcgca gtcgatctgg tgcgcgtgaa tgaaatcagc gatctcggca
    11101 tagttctcgt tacccagtcg gagcagggtt cgcatctcgt ccgccgggta ccgggccaga
    11161 ccgttctcga ggccatgagt tagcgacggc tcgaggaatc ccccatttcg gccggtcgcg
    11221 ccgaaggcga tccgctccat ctcgagcagc acgacatcgc gatttgggtc gcgctgcttc
    11281 gcctcgatcg ccgcccagag gcccgtgaat ccgccgccga tgatgaccag atcggccgtg
    11341 gcattcgtcc gcaatggcgg ctggatcggc acgggacgag gttggctggt ccaatacacc
    11401 gccggctcgg cgtcgacaag cgatgtctgc gggctccaca tgccgggatg gtacctgaaa
    11461 tgattgggac cgattcatac cagcctcggc tcgtatcact ccagattgcc ttctggtcac
    11521 aaattgcgct ccgcacggcc ttgtcgcggc cgccacactc accggctctg ggggtagccc
    11581 agtcacaggt cttctcgttg gcagagtgct aaggtcaccc cctgcgttcg cgctgaccgt
    11641 gcgaacgaga gcacacaagg aggctatctg tatggaaacc ccgatgaccc aatatcagtt
    11701 ccgcgccggc gatgacgtga tcgcggccga cggagataag attggaaacc ttgctgatct
    11761 tcacggaaca tatctcgtcg tggaaaaggg cttctttttc ccgcgcgact actacattcc
    11821 cttcagcacg gtcgcgaact acgatccggg cgacggcaag atctaccttt ccgtcaccaa
    11881 ggacgccgcc ctcaacagcg gctgggacca caagccggac ctctcggccg aggaccgcgc
    11941 gtacgccgcc ggcgccccgc gcgacactta cgccacagat acgacggcaa cgaccaccgg
    12001 ccggactcgc gacacctacg cgactgatcg gacgaccgag gttaccgatc aggatcacat
    12061 cagcgtgccg gtgcacgagg aagagttgat cccgaccaag acggtccgcc aggccggcca
    12121 ggtccgcgtt gacaaggacg tgatcgagga agagcgcgag atcgatgtac cggtgaccga
    12181 ggagcgggtc aaggtcaccc ggcgcgcggt cgaccaggtg gctgacaccg gcgatatggc
    12241 gttccgggag gagagcttcg aagtcccggt ccgcaccgag gatgtcgggg tcgacaaggt
    12301 ggtccgggtc gctgagcagg ttgatatcga caagcaggcg atccaggaca ccaagcgcgt
    12361 ctccggtacc gtgcgcaagg agcgcgtcga cgtggatgag gcgggcaccg atgcctcgat
    12421 ctacgcagat accaaccgcg tcacggacga tacagccagc acgtaacttt cccccttcac
    12481 cgacgaatgg cggacccggt aaccgggtcc gccattcgtc tgcccgccgc gcggtcgggt
    12541 atcgttgcgc cccgaaccgc atacggagtc gcgcgcatga ccaagcctgt ctttgaaacg
    12601 gagcgcctaa tcgtccgcga gtacacggtg gacgatgcac ccgctatgtt cgacatctac
    12661 aaggacccgg aggtctggcg atatctcgcg ggcggcgacc cgtataccag cgtcgggcaa
    12721 gcccgggagc ggatcgagct ccggatccag cggtaccggg agggcgcgcc gtatggcctg
    12781 tgggctgttg tcgtgcgcga cacagcgcag ctgatcggct ccgttttgct ctccccgctc
    12841 gaagatggcc ccgacgtcga ggtcggttac cacttcggcc ggttcgcatg gggcaatggg
    12901 tatgccaccg aagtgacgcg cggcgcgatc cggtacggat tcgacgtact cggcctgccg
    12961 agcatctgcg gcgtcgtttt tcaggaaaat gccgcctcgc agcgcgtcct cgagaaggcc
    13021 ggcatgacct atcgcggcct gcgctccgtc ttcggcttcg acggcgtcat gtactacaca
    13081 atcgagtccc ccaccggctg cgatcaaccg ggatagattc ccgacctgat cgggaatccc
    13141 gcctctcgcg ccgggaaatc cccgaccata ccgtggctcc tgacgtattc gatcaccagc
    13201 aggagcagtc accatgaacc gacgcgcgtt cctggccggc tcaaccggtc tcgcttgcct
    13261 tgccaccctc ggaccacgca ctgcatcggc tctcgcccac gccgcaagca cgccggttgc
    13321 cgaagacacg ttcccgacgc ttgcgatcgg cctcaaagac gatggcttcg agctcccgga
    13381 cgccatccag gccggtcgtt atcacgtcac cgtcacgaat cacggcacct cagatgcaag
    13441 ccactcagcg cttgggcgga tcccggatca ggtcaccgac gcgcagtacc aggaatggct
    13501 cgactccctg tcgcgcaccg atggcaccga cggccagacg gacgcgctca cctgggacga
    13561 catcgagttc gtaggcatgc ccgactggcc gaaggccggc cagagcgtat ccggcgtcat
    13621 cgatctggcc cccggccgct acttcctgtt cgatcccttc tcctcacggg gcgacatgtc
    13681 gttgacgatc accggcgatc cagtttccgc tccggagcct gagtcggacc tgacggtcac
    13741 gctcgtcgac atgaaaatca atcttcccga cgcggccata acgtcgaagc cggtgcgctg
    13801 gaagatcgag aacaccggct cgatcagcca tgaagtcgcc gtcattccgg tcacccccac
    13861 gttcactgaa gaagatctca ccgtcctctt gtcgctgccg gaggacggaa ctccacctgc
    13921 gggaacggat aacctcgact atcagccggt cgccgcgatc gggatcctgg ccaagggcca
    13981 caccagctgg ctcgacgtca agctcacccc cggtcactat ctcgcggcct gcatgctgcc
    14041 gttcgggacc gggtatccgc acgcgatgga tgggatgtac cgcttcttcg aagtgaagta
    14101 gattgcacag agttgcgcag gccactgtct gattgctacg ccgcccggcg aaggcgtgat
    14161 ggaccgccgg gcgacatccc tgacgggtga accccatggc agcgcctcga gcagtagtga
    14221 cgcagacacc aggactccgg gcccggttca tccgcgactg gccggccgca gcctgggttg
    14281 cctccgctat cgtcatcaac atcgccgggg ccaggagcgc gccacagtcc tggcaccaga
    14341 tggtcgcctt gaccgatatc ccggcatctg accgcgctga attacggcgg tccctcgccg
    14401 atctgcatct gagtccggtc ttcctcgcgt gtttccagat cgccacagcg gtcatcggca
    14461 ccatcgtgaa cctcatcatc ggctggctgc tgattcggcg ggcgccccgc acgggcttcg
    14521 cgatctacct cgccttcatc atgctggcga tgaccaacgc gaactacccg ccgtcgatcg
    14581 ccgacatgct gcccggtcag ccggtcgccc aattcgttat tcggctctcg acagcagtgg
    14641 cgatttccgg gttcttcacc ttgccgttcc tttttcccga tggtcgcttt gtcccgcgtt
    14701 ggaccgtcct ttgctggata tacgtggtca tcggcgtctt tacctttgcc ttcttcccgt
    14761 cgtacaagcc gggcggttca acctgggata tcgccaacgc gatcggcaca gtcctgctga
    14821 tcctatcgct cctattcgcg gtcatctacc gatatcgacg agtctcgacc ccgctgcaga
    14881 agcggcagac gaaatgggtc gcgttcggtc tcggtgtcag cgttccggcg ttcttcgctg
    14941 ctgacgcgat gatgcgcaat atcgatgcca caccggccgg catcggctgc ctgctcgcgt
    15001 tcctggtcat catgccgatc gccac
//
LOCUS       DATAOZ010000142         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1901264, whole genome shotgun sequence.
ACCESSION   DATAOZ010000142 DATAOZ010000000
VERSION     DATAOZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1901264"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VHR64_03985"
     CDS             <1..464
                     /locus_tag="VHR64_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302016.1"
                     /translation="IRLQFGRILIAQAAVGCAAIGIIVATATPEMSRASWFPETIGNR
                     PSAEVLIYLVGFLIGAGFSVQRMALADLFDTTTESMRWTALAASAVACLAPLVVGWAS
                     REFAENWVLVAGIVLAMAIMATLGVLPGNSPAHRSSPAAPAPMRQALLPRQ"
     gene            480..1421
                     /locus_tag="VHR64_03990"
     CDS             480..1421
                     /locus_tag="VHR64_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX3302017.1"
                     /translation="MTAPARVPSISAIGVVSWDEIYVLDRYPHEGHFSLISDIRSGPG
                     GTTGNIAMAAGKLGAEVKLVASVGQDELGNRILDSLGAAGIDTSGCQRHEGETDLSVM
                     LVSGETAERTILWKQAPTIKRRDRIDIDALFQADITVIDCVDYELRRFLSDLPAHTRP
                     AARLLGTLTYLADVVADDKLEIALRHDILVGNEREYQELVGCDNAEYCLSAVASAMPG
                     AHLRLAVMTCGDRGSVAATRDQVIRVPARRVAAVDATGAGDSFTGALAYALALRWDLE
                     RSLLFANSVASFVVTTIGAQTSLPSLAEVLAAAGLEP"
     gene            1418..2569
                     /gene="queG"
                     /locus_tag="VHR64_03995"
     CDS             1418..2569
                     /gene="queG"
                     /locus_tag="VHR64_03995"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX3302018.1"
                     /translation="MNVRNPTELSDAIKELARAEGLAVASITTAEPFPGLTELLDSRI
                     EAGHLHGMDWFTPERSVVSANPRMLHERAQSIISVGIPYYRSDIQKPDDGVKRGRIAR
                     YAWGADYHKTLKNRMARLAARIESKIGGPMEVRLLVDTARIVDRAVAARSGLGWYGKH
                     TNIIVPGHGSFVMLGEILIDLELEPDQPLQKDCGRCQICLNRCPTGAIVAPYTVSAPL
                     CISYQTIENRGMIPLDVRGKMDDWVFGCDVCQDVCPYTGAAKSMIDEAFRPATIDNAF
                     PSLHWLLQLSEEEFRSVYRGTAVLRTKRAGLARNAAIALGNSGDPDDLPVLADALRSY
                     DLPLVRAHAAWAMGRIGGESARIALGSAGHSDPDPEVRAECRSALEIAA"
     gene            2612..2986
                     /locus_tag="VHR64_04000"
     CDS             2612..2986
                     /locus_tag="VHR64_04000"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX3302019.1"
                     /translation="MSSTGKHIAGQVDIAESAIAELVRDAVKTCYGVVGIGSTASRRL
                     PARLPGPWRRQPIVISVNSGRISIAIPIIVQYGTPIATVARNVIQTVGFQLRHSLGLD
                     VDRVDVRVSGLRHEPSDGNPEP"
BASE COUNT          519 a          980 c          949 g          543 t
ORIGIN      
        1 cgatccggct ccaattcggg cgaatcttga tcgcccaggc cgccgtcggc tgcgcggcta
       61 tcggcatcat cgtcgctacg gcaacaccgg agatgtcgcg cgcgagttgg ttcccggaaa
      121 caatcggtaa ccggcccagc gccgaagtac tgatctacct ggtcggcttc ctcatcgggg
      181 caggtttttc agtgcaacgg atggcgctgg ccgatttgtt cgacacgacc acggaaagca
      241 tgcgctggac ggcgctcgct gccagcgccg tggcctgcct ggcgccgctg gtcgtcggtt
      301 gggccagccg cgagttcgcc gaaaattggg tgctcgtagc cggcatcgtg ctggccatgg
      361 caatcatggc cacgctgggg gtactaccgg gcaactcgcc ggctcaccgt tcttcgccag
      421 cagcgcccgc gcccatgcgg caggcgctgc tccctcgcca ataaggcgat gccacagcga
      481 tgacggcacc agcgcgtgtc ccctctatct ccgcaatcgg tgtcgtcagc tgggatgaga
      541 tctacgtcct cgatcggtac ccgcacgaag ggcacttctc gctcatctcg gatatccgaa
      601 gcggaccagg gggaacaacg ggcaacatcg cgatggccgc cgggaagctc ggcgctgaag
      661 tgaaactggt ggcatcggtc ggccaggacg agcttggcaa ccgaatactg gattccctgg
      721 gtgcggcagg gatcgacacc tctggttgcc agcgccatga gggcgagacc gatctctcgg
      781 tcatgctcgt ctcaggcgaa accgccgagc gaacgatcct ctggaagcag gcgccgacga
      841 tcaagcggcg ggataggatc gatatcgatg cgctgttcca ggcggacatc accgtgatcg
      901 attgcgtgga ctacgaattg cgccggtttc tgtccgatct cccggcccat acgcggccgg
      961 ccgcgcgcct gctcggcact ttgacctatc tggccgatgt tgttgcggac gacaaactcg
     1021 aaattgccct tcgacatgac atcctcgtcg gcaatgagcg cgagtaccag gaactggtcg
     1081 gatgcgacaa tgccgaatat tgcctgagcg cggtggcaag cgcgatgccg ggcgctcatc
     1141 tccggctggc agttatgacg tgcggcgatc gaggctccgt agccgcaacc cgtgatcagg
     1201 tcatccgggt tcccgcccgg cgcgtggctg ccgtggacgc caccggagcc ggcgactcct
     1261 tcacaggcgc gctcgcgtac gcgctggcat tgaggtggga cctcgaacga tcgttgctgt
     1321 tcgcgaattc tgtcgccagc ttcgtcgtta ccaccatcgg cgcccagacg tcgctgccat
     1381 ccctcgccga ggtgctggct gcggctggac tcgagccgtg aacgtcagga atcctaccga
     1441 gctcagcgat gccatcaaag agctcgcgcg tgccgagggg ttagcggtcg cctctatcac
     1501 gaccgccgag ccgtttcccg gcctgaccga gctccttgat tcgcggatcg aggccggtca
     1561 tttgcacgga atggactggt tcactccgga gcggtctgtc gtctcggcca acccgcgcat
     1621 gctgcacgag cgcgcgcaat cgatcatctc ggtcggcatc ccctattacc gcagcgacat
     1681 ccagaaaccc gacgacggcg tgaagcgggg ccgaattgcc cgctacgcct ggggtgctga
     1741 ctaccacaag acgctcaaga accggatggc ccgcctcgca gccagaattg agtccaagat
     1801 cggcgggccg atggaggtgc gactgctcgt cgataccgcg cgaatcgtgg accgcgctgt
     1861 ggcggcccgt tctggcctgg gctggtatgg aaaacacact aacatcatcg ttcccggaca
     1921 cggttcattc gtgatgcttg gcgagatcct gattgacctg gaactcgagc ccgaccagcc
     1981 cctgcagaag gattgcgggc gctgccagat ttgcctgaat cgctgcccga ccggcgcgat
     2041 cgtcgcgccg tacacggtca gcgcgccgct ctgcatctcc taccagacaa tcgagaaccg
     2101 cggcatgatc ccgctcgacg tgcgcgggaa gatggacgac tgggtcttcg gctgtgatgt
     2161 ctgccaggac gtctgcccgt ataccggcgc ggccaaatcg atgatcgacg aggcattccg
     2221 gccggcaacc atcgacaatg ccttcccgtc gctgcattgg ctcttgcagt tgtccgaaga
     2281 ggagttccgc agcgtgtacc gaggcacggc cgtcctccgg acaaagcgcg cggggctcgc
     2341 ccgcaatgcg gcgatcgcgc tcggcaactc cggcgatccg gacgacctgc ccgtcctggc
     2401 tgatgcattg cgctcctacg atctgccgct ggtccgtgcc catgcggcct gggccatggg
     2461 caggatcggt ggcgaatccg cccgaatcgc cctcgggtcg gctggacact cggaccccga
     2521 tcccgaagtc cgggcggaat gccggtcagc actcgagatt gccgcctagt tcggggtcgt
     2581 ttacccggac cggggtatga tgtgccgcga catgagcagc actgggaagc acattgccgg
     2641 acaagtcgat atcgccgaga gcgcgatcgc cgagttggtc cgcgacgcgg tcaagacctg
     2701 ctatggcgtg gtcggcatcg ggtcgaccgc gtctcgtcgg ttgccggccc gcctacccgg
     2761 tccgtggcgt cgccaaccga tcgtgatctc ggtgaacagc ggccggatct cgatcgcgat
     2821 cccaatcatc gtccaatacg ggacaccgat cgcgaccgtc gcccgcaacg tcatccaaac
     2881 tgtcggcttt cagcttcggc actcgctcgg cctcgacgtt gaccgggtcg atgtccgcgt
     2941 gtccggactc cggcacgagc cgtcggacgg gaacccagag ccgtgacgca t
//
LOCUS       DATAOZ010000143         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2689748, whole genome shotgun sequence.
ACCESSION   DATAOZ010000143 DATAOZ010000000
VERSION     DATAOZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2689748"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VHR64_04005"
     CDS             <1..824
                     /locus_tag="VHR64_04005"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302020.1"
                     /translation="TPLAVRHGDISGAKNIANALVAQNGVQATILAQYGMTGPLDLFE
                     NRHGLKSVFPGINWMESITAPLAADSYIMGCHVKAFPCLATGQGIVAAGIAVHQQISE
                     DPDRLKEITVAIADTPSLQRQKDDPGRIDPQSREAADHSFNFLAAVALIDGKFGLNSF
                     DNDRWNDPKVRAVMSRLSVVCDASLNGRSPGAFPCAIRATGSDGRSYLAEVLDPPGFS
                     RKGIDQQAVLDKFHSITEDRLGPGSRQRIIDAAMSLEKSASCADLMAAVASASKA"
     gene            complement(851..1828)
                     /locus_tag="VHR64_04010"
     CDS             complement(851..1828)
                     /locus_tag="VHR64_04010"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3302021.1"
                     /translation="MLVPRRHLLQLGVCAAALRVSPGIAQAQAYPTRPVRFVVGFVAG
                     GPNDILARLIGEWLSDRLGQPFVVENLPGASSNTATEAVVRAPADGHTLLLIGPANAI
                     NASLFPNLSFDIRRDIAPVAGLTREALVLVVHPTVPASTVPQLIAYAKANPGKLRMAM
                     TSSGSSPHVSGKLFKLMTGVELTEVPYEGGGPALRDLIAGQVDMMFEPMSASIRPIRA
                     GSLTPIAVTTAARSEALPDVPTMGDLVPGYEASAVTGVGVPRNTPLEIIDTLNKTINA
                     AYVDPKMKARLADTGGGLLPGTPTEFGMVLAQEIEKWARVVKASGMKPQ"
     gene            complement(1961..>2144)
                     /locus_tag="VHR64_04015"
     CDS             complement(1961..>2144)
                     /locus_tag="VHR64_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3302022.1"
                     /translation="KDRPEVVYVLSGKIIDHRGDVAKEYGAGESITAGKDTTHWMENK
                     GTVPAVLIVSTIAKKE"
BASE COUNT          383 a          713 c          668 g          380 t
ORIGIN      
        1 tcacgccgct ggcggtgcgc catggcgaca tttccggtgc caagaacatc gccaacgcgc
       61 tggtggctca gaacggcgta caggccacga tcctggccca atacggcatg accggcccgc
      121 tcgatttgtt cgagaaccgg cacggtctca aatcggtgtt ccccggcatc aactggatgg
      181 agtcgatcac cgctccgctg gcggccgaca gctacatcat gggctgccac gtcaaggctt
      241 tcccctgcct cgcgaccggc cagggcatag tcgcggccgg aatcgcggtg caccagcaga
      301 tcagtgagga tcccgaccgg ctcaaggaga tcaccgtcgc gatcgccgac acgccttcgc
      361 tgcagcgcca gaaggacgac cccggccgca tcgatccgca atcgcgcgag gccgccgacc
      421 acagcttcaa cttcctggcc gcggtcgccc tgatcgacgg caagttcggc ctgaactcgt
      481 tcgacaacga ccgctggaac gaccccaagg tgcgcgccgt catgtcccgg ctttcggtcg
      541 tgtgcgacgc tagcctcaac ggccgctcgc ctggcgcctt cccgtgcgct atccgcgcga
      601 ccggcagcga tggacgcagc tacctcgccg aggtgctcga tccacccggc ttctcccgta
      661 aaggcatcga ccaacaggcc gtgctcgaca agttccattc gatcacggaa gaccgcctcg
      721 gcccgggctc acgccagcgc atcatcgatg ccgcgatgtc gcttgagaag agcgcgtcgt
      781 gcgccgacct gatggcggcg gtcgcaagcg cgtcgaaggc gtaaagcggg acactacgcg
      841 tgcgtgcggc tcactgcggc ttcatgccgg aggctttgac taccctcgcc cacttctcga
      901 tttcctgggc gaggaccatt ccgaattcgg tcggtgtacc tggcagtagg ccgccgccgg
      961 tgtccgccag acgcgctttc atcttgggat cgacatacgc ggcgttgatc gtcttgttga
     1021 gagtgtcgat gatctcaagc ggcgtgttgc ggggcactcc gacaccagtg accgcgcttg
     1081 cctcgtagcc tggcactaag tcgcccatcg tcggcacgtc gggcaacgct tccgagcgtg
     1141 ccgcggtggt caccgcaatc ggagtcagcg agcccgctcg gatcggcctg atcgatgcgg
     1201 acatcggctc gaacatcatg tccacctgcc cggcgatcaa gtcgcgcagc gccggcccgc
     1261 caccctcgta gggcacctcg gtcaactcga ctccagtcat gagcttgaac agcttgccgg
     1321 acacatgcgg cgagctgcca ctgctggtca tggccatccg aagcttgccc ggattggctt
     1381 tggcataggc gatgagctgc ggaacagttg acgctgggac cgtgggatgg acgaccagga
     1441 cgagcgcctc gcgggtgagg cccgcgaccg gagcaatatc gcgacggatg tcaaagctca
     1501 gattgggaaa gagcgaggcg ttgatggcgt tggccgggcc gatcagcaga agcgtgtggc
     1561 catcggctgg cgcgcgcacg accgcttcgg tcgcggtgtt gctcgatgcg cccggcagat
     1621 tttcgaccac gaagggctgg ccgagccgat cggacaacca ttcgccgatg agacgggcaa
     1681 ggatgtcgtt cgggccaccg gcgacaaagc cgactacgaa ccgcaccggt cgcgtcggat
     1741 aggcttgcgc ctgcgcgatc cccgggctga cccgcaacgc ggcagcgcac actccgagct
     1801 gcaacaaatg gcgacgcggg acgagcatga cgttcccctc gctcgacaaa gttacctcaa
     1861 tctcgcaggg atgcgaaaga gggggacgat cactattggc ccgctgcgtc gcctcgcggc
     1921 atgcgagcga gcaacccggt cggatcagtt ttggcgggcg ttattccttt tttgcgatcg
     1981 tcgacacgat cagcacggca gggactgtgc ccttattctc catccagtgc gttgtatcct
     2041 tgcccgccgt gatagactcg cctgcaccat actccttggc cacatcgccg cggtggtcga
     2101 tgatcttgcc tgacagcacg tataccacct ctgggcgatc cttg
//
LOCUS       DATAOZ010000144         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4266352, whole genome shotgun sequence.
ACCESSION   DATAOZ010000144 DATAOZ010000000
VERSION     DATAOZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4266352"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VHR64_04020"
     CDS             complement(<1..1096)
                     /locus_tag="VHR64_04020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3302023.1"
                     /translation="MRVLMLSDFYRPLTGGVEVHVEGLSRALVARGHDVTVATLLTDG
                     LPREEVIDGVRVRRLESAVSRVPGLFANSARPWAPPFPDPMLARALRRAVSEVRPDVV
                     HGHDWIARSYLPARVRHSAAFVLTLHYYTLACARKDLRYKGEICSGPGVRKCLRCASA
                     HYGAVKGPMIAAGNWPVSRLEVRAADVVIAVSAATARGNGIRKDDPRCVVIPNFLLDQ
                     GEPSDDDSPLLDQLPPRPFVLFVGDLRRAKGIDVLFDAYRRLPEPPALVLIGDVGPET
                     PADIPAGACVLSGWPNQAVRAAWERCLFGVLPSVWAEPFGLVLLECMAAGRPVIASRV
                     GGIPEVVADGETGILVPPGDAAELARAMERL"
     gene            complement(1096..2175)
                     /locus_tag="VHR64_04025"
     CDS             complement(1096..2175)
                     /locus_tag="VHR64_04025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3302024.1"
                     /translation="MTARYVPEIGGTEVHTRELAERLARRGHRVTVLTTDRSGNLPVR
                     EERGGVTILRVRAWPAERDYYLSPGIVGAMRSSPYDVVHIQGYHTLVAPVAMLSAMRS
                     NIPFVLAFHSGGHSSRVRRAMRGPQIRALRPLFGRAHRLVASSASELQYFKWRLGMPA
                     AKFEMVPVLANLPQIADGVQDPFGNTLLVTVGRLEHYKGHQRVIEALPLLRGRVPNIR
                     LRIVGEGPLESRLYQLAREHHVEDLVTIAAIPPTHRREMAEIMQRAALVISLSDFESA
                     GLSIREALSLGRPVLVTDIAGYSDLHGSPLVNAIPLKSPIPVIANAIADSLRRTVVAE
                     AADIPTWDECIDQFERIYRDAIGAR"
     gene            2597..3364
                     /locus_tag="VHR64_04030"
     CDS             2597..3364
                     /locus_tag="VHR64_04030"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009312 - oligosaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009877 - nodulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3302025.1"
                     /translation="MGSDHKRLLEEMYQRTEDPWEYAHSEYEQEKYASSLAALPGTRY
                     GAALEIGCSEGVFTNLLAERVDRLLAVDVSDIALTRARFRCASHRHVEFRQFDLVADD
                     LAEHFDLIVCSEVLYYVPPFKRLEVARRIVRMLKPGGTLLLVHTLRSHTRTWPDIYGE
                     GGAERLHRVFTRAFALPVLARHFADGYEIVVATSSGPELARLRRTFETWGVRIRNVYP
                     GLRREVVIRLRRHPLPFRMATRAHARLTRLRRVGETE"
     gene            complement(3402..3677)
                     /locus_tag="VHR64_04035"
     CDS             complement(3402..3677)
                     /locus_tag="VHR64_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302026.1"
                     /translation="MITPLVNSRGRQFGFVADEAVYDTRGRRLGTRNGARVVNERGAE
                     IGEISGNTYSSHRPTSGVRATFSAPVTFVTGWVRGIRAGKLHPLTDA"
BASE COUNT          742 a         1242 c         1200 g          670 t
ORIGIN      
        1 tcagccgctc catcgcgcgc gctaattccg ccgcgtctcc cggcgggacc aggatgccgg
       61 tttctccgtc cgcgacgacc tccgggatcc ccccaacccg gctggcaatc accgggcggc
      121 cggcagccat gcactcgagc aggaccagcc cgaacggctc tgcccaaacc gacgggagca
      181 ccccgaacag gcagcgctcc caagccgccc gcacagcctg gtttggccac cccgacagaa
      241 cgcacgcccc ggccggaata tctgccggcg tctccggccc gacgtcgccg atcaggacca
      301 gcgcgggcgg ttcaggaaga cgccggtacg catcgaaaag gacatcgatc cccttcgctc
      361 gccggaggtc gccgacgaac agcacgaatg gccgaggtgg gagttggtcc agtagaggcg
      421 aatcgtcatc cgagggctca ccttgatcga gcagaaagtt ggggatgacg acgcatcgcg
      481 gatcgtcctt tctgatcccg tttcctcgag cggtcgcagc gctgaccgcg atcacgacat
      541 cggccgcgcg gacttcgaga cgagagacgg gccagttgcc ggcggcgatc atcgggccct
      601 tgaccgcgcc gtaatgagcg gatgcgcagc gcaggcactt ccgcactccg ggaccggaac
      661 agatctcgcc cttgtaccgc agatctttcc gcgcgcacgc aagcgtgtag taatgcaacg
      721 tgagcacgaa tgctgcactg tgccgaacgc gcgcaggcag atacgaccgc gcgatccagt
      781 catgtccatg cacgacgtcg ggacgcactt cggataccgc tcggcgcagg gcccgggcca
      841 gcatcggatc cggaaacggc ggcgcccatg gacgggccga gttggcaaac agtccgggca
      901 cacggctcac cgccgattcc agcctccgaa cccgaacgcc gtcgatcacc tcttcccggg
      961 gtagaccgtc ggtcaggagc gtggccacgg tcacgtcgtg accacgcgcg acgagggccc
     1021 ggctcagccc ctccacatgg acttcaacgc caccggtgag cggtcggtag aagtcggaca
     1081 gcatcaggac tcgcatcagc gtgcaccgat cgcatcccgg tagattcgct caaactggtc
     1141 gatgcactca tcccaagtcg gaatgtcggc cgcctcggca acgaccgtcc ggcgcaggga
     1201 gtccgcaatc gcattggcta tgacggggat cggactcttg agcgggatcg cattgaccag
     1261 cggtgaacca tgcaggtcgg agtagccagc tatatctgtt acgagaaccg gccgcccgag
     1321 cgagagcgcc tcccggatcg acaggccggc cgattcgaag tcgctcaggg aaatgacgag
     1381 cgcggcccgc tgcatgatct cggccatttc ccggcgatgc gtcggcggga ttgcggcgat
     1441 cgtgaccaga tcctcgacat ggtgttcccg ggcaagttga tagaggcgcg actcaagcgg
     1501 accttccccg acgattcgaa ggcgtatatt ggggaccctg ccccggagca gcggaagtgc
     1561 ttctataacc cgctgatggc ccttgtaatg ctcgagacgg ccgaccgtga ccagcagggt
     1621 attgccgaac ggatcctgga cgccgtccgc gatctgcggc aagttggcga gtaccggcac
     1681 catttcgaac tttgccgccg gcatgccaag tcgccatttg aagtactgga gctcggacgc
     1741 gctgctggcg accagacggt gcgcgcggcc gaagagtggc cgcagagccc tgatctgggg
     1801 gccgcgcatc gctctccgca cgcgcgacga gtgaccgccg ctgtggaagg cgagaacgaa
     1861 cggaatattc gaccgcatcg ccgagagcat cgcgactggg gctaccaacg tgtgataccc
     1921 ctggatgtgg acgacatcat acggagacga cctcatcgcg ccgacgatgc cgggcgaaag
     1981 gtaatagtcc cgttcagccg gccaggcacg cacgcgaagg atcgtcaccc cgccccgttc
     2041 ctcgcgaact ggaaggttcc cgcttcggtc ggtcgtcagt acggtgacgc gatggccgcg
     2101 cctggccaac cgctccgcca gctcacgggt atggacctcc gtaccaccga tctcgggaac
     2161 ataccgtgcg gtcacgagca ggatcgacag cgaccgatcg agagagtttc gggacccgac
     2221 ccgcgcccct ggttgggccg gggcccctga ttcagccggg ttgttcattc gttcgcgcgc
     2281 ctggcgcccg attggaggta gtccttcatc ggacccgagt tgcctccgaa gtcagccgtc
     2341 tccagtacga gtacgaacca gatcggcgcg gctgagccac gatatgagta cgaatgcgac
     2401 cgtcatgtgg tgcagtcatg agcgtactgc gaatgcgatg accgcagacg tatattcaat
     2461 actattgctg aagtatttgg gcaccagcac aacatttgcc caaatctacc gatcgggcta
     2521 cctacctatg tacctcagcg gcacgagccc ggcaaaactg attcatcgct cgtctgtgaa
     2581 aggtggtttg gcgacagtgg ggtccgatca caagcgtctg ctcgaagaga tgtaccagcg
     2641 caccgaagat ccctgggagt acgcgcacag cgaatatgag caggagaaat acgcgtcctc
     2701 gctcgccgca cttccgggaa cacgatatgg cgccgcgctt gagatcggat gcagcgaggg
     2761 tgtcttcacc aatctcctgg ctgagcgcgt cgatcgactc ctggcggtcg atgtttccga
     2821 catcgccctc acacgggcca ggttccgatg cgcgagtcac cggcatgtcg agttccgcca
     2881 attcgacctg gtcgcggacg atctggccga gcatttcgac ctgattgtgt gctcagaggt
     2941 gctttattac gttcccccat tcaagcgatt ggaggtcgcc cgccgaatcg tccgaatgct
     3001 caagcctggc ggaaccctgt tgctcgtaca cacgcttcgg tctcacacac gaacatggcc
     3061 ggatatctac ggcgaaggcg gggcggagcg actgcaccgg gtctttactc gcgcattcgc
     3121 tttgccggtg ctggcgcgac atttcgcgga cggctatgag atcgtcgtgg cgacctcgag
     3181 tggcccggag ttggcgcgct tgcgtcgaac gttcgagacg tggggcgtac ggatccgaaa
     3241 cgtctatccg ggcctccggc gagaagtcgt gatccgcctg cgtcgccatc cgcttccgtt
     3301 tcgcatggca acgcgcgccc atgcacggct cacccgtctc cgccgggttg gcgagacgga
     3361 gtgaaccggt taggttgtgc tctttggagt ggtaccggcg tctaggcgtc ggtcagcgga
     3421 tgcagcttgc cagcccgaat gccgcgcacc cagccggtga cgaaggtaac gggcgctgaa
     3481 aaggtggcgc gcacacctga tgtgggccgg tgcgagctat acgtgttgcc ggagatttca
     3541 ccgatctcgg cgccgcgctc gttgaccacc cgggcgccgt tccgtgtgcc gagtcgccgg
     3601 cccctggtgt cgtagaccgc ctcatcggcg acgaatccaa actggcgccc acgcgaattc
     3661 acaagcggtg tgatcattgg aacagccttt ccctccgcgc gttatcccgc cgaccgctga
     3721 cgacccatcc gtcgaaaata cattgtacgt acagtaatac tagcgtacgt acagcggtct
     3781 gtgaagagga gtaacggcgg aaactcccca tccgggagtc ccagaaccaa ccagccattg
     3841 gtctcgagtt agta
//
LOCUS       DATAOZ010000145         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6636880, whole genome shotgun sequence.
ACCESSION   DATAOZ010000145 DATAOZ010000000
VERSION     DATAOZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6636880"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VHR64_04040"
     CDS             <1..1209
                     /locus_tag="VHR64_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302027.1"
                     /translation="GARPLHADAAGADDRRCARRVSAANREHGLCDGDGLLHELRRDG
                     VRLDLIRATGHDKHVLRDYRLCKDLGFGTIRDGLRWHLIEKSPGQYDWSSWLPALEAA
                     ESVGLEVIWDLFHYGSPDCIDQAAPDFPERFTHFAMAALEVQQSATGRSPLICPLNEI
                     NFLSWAVDDGYFPRVGPEERGWFKRQLVRTAIIASRAVKQTWPHSTIVGAEPLIHIAP
                     HDRRRKTVRAAEQNLRGMYEAYDWILGMAEPELGGDSSLVDVIGFNFYPHNQWYYEGP
                     TVPMGHHEYRPLADMLLEMNERYKKPMFLSETGAEGSAKPSWLHYVSNEVRDAMSRGA
                     DIRGICWYPITAYPGWDNSRHAETGLLSTIVADGSRHVDERLLEEFNVQRALLARTPE
                     SVSVVGGSRR"
     gene            complement(1206..>1853)
                     /locus_tag="VHR64_04045"
     CDS             complement(1206..>1853)
                     /locus_tag="VHR64_04045"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX3302028.1"
                     /translation="SDSPAGPFVDMGMPLLLGVGFEYIDPMVFADPESGKWLLYWGSG
                     FHPIKVQELGPDGMSFAPGSEPISLIWPNPVKGAFPRLVEAAWVIRHDEFYYLFYSGD
                     NCCGPDAEYGVMVARSREPTGPFETLEESRGVPHSLMLFKSERWLAPGHNCIIQDEAG
                     ETWIIYHAIDVNRPRQRQEDEINSRRILLIDRIEWRDGWPHVGTPSDEPRPAPIV"
BASE COUNT          344 a          575 c          585 g          349 t
ORIGIN      
        1 ggcgcgcgtc ctcttcacgc agacgcggct ggagccgacg accgccgatg cgctcgcagg
       61 gtcagcgcgg caaatcgcga gcacggtctc tgcgacggtg atggtctgct acacgagctc
      121 cggcgcgacg gcgtgcgcct cgacctcatc cgggcgaccg gccacgacaa gcatgtcttg
      181 cgcgactatc gcctgtgcaa ggatctcggc ttcggaacga tccgggatgg cttacggtgg
      241 cacctgattg agaaatcgcc cggccagtat gactggtcga gctggctgcc cgcgctcgag
      301 gccgccgaga gtgtcgggct tgaggttatc tgggacctgt ttcactacgg ttcgcccgat
      361 tgcatcgacc aggcggcgcc cgatttcccc gagcgcttca cgcacttcgc gatggctgcg
      421 ctggaggtcc agcaatcggc caccgggcgg tcgccgctga tctgcccgtt gaatgagatc
      481 aacttcctgt cctgggccgt ggatgatgga tatttccctc gcgttggccc ggaagagcgt
      541 ggctggttca agcgtcagct ggtgcgaacg gcgatcattg catcgcgggc cgtaaagcag
      601 acctggcccc attccacgat cgtcggcgcg gaacctctga tccacatcgc gccccatgat
      661 cgaagacgca agacggtgcg cgcggccgag cagaatcttc gggggatgta cgaggcctat
      721 gactggattc tcgggatggc cgagccggaa ctcggcgggg acagttcgct tgtcgatgtg
      781 atcggcttca atttctaccc ccacaaccaa tggtattacg aaggaccgac cgttccgatg
      841 ggccaccatg aatatcgccc gctcgccgac atgctccttg aaatgaacga gcggtacaag
      901 aaaccaatgt tcttgtcgga gacaggcgcg gaaggctcag ccaagccttc ctggctccat
      961 tatgtgtcca acgaggtgcg cgacgcgatg agccggggcg ccgacatccg cggcatctgc
     1021 tggtacccga tcaccgctta tcccggctgg gacaacagcc gccatgccga gaccggcttg
     1081 ctctccacga tcgtcgcgga cggcagccgt cacgtcgatg agcgcctgct ggaggaattc
     1141 aatgttcagc gggcactcct cgctcggacg cccgaaagtg tctctgtcgt cggaggaagt
     1201 cgccgctaga cgattggtgc cggccgcggc tcgtccgaag gggttccgac gtggggccag
     1261 ccgtcgcgcc attcgatgcg gtcgatcagc aggattcgac gactgttgat ctcgtcctcc
     1321 tgcctttgcc gggggcggtt cacgtcgatc gcatgataga tgatccaggt ctcgccggcc
     1381 tcatcctgga tgatgcaatt atgtccgggt gcgagccagc gctcgctctt gaacagcatc
     1441 aggctgtggg gcacgcctcg cgactcctcc agggtctcga acggaccggt cggttcgcgc
     1501 gatcgggcaa ccatcacgcc atattcggcg tcgggtccgc agcaattgtc gccggaatag
     1561 aaaaggtaat aaaactcgtc atgacggatg acccaggcgg cctcgacgag ccgcgggaat
     1621 gcacccttta ccggattggg ccagatgagg ctgatgggct cgcttccggg cgcaaacgac
     1681 atcccgtcag gcccaagctc ctgcaccttg atcgggtgga atccggaacc ccaatagagc
     1741 agccacttgc cgctttcggg gtcagcgaac accatcggat cgatatattc gaagccaacg
     1801 ccgaggagga gcggcatgcc catgtcgacg aatggtcctg ccggtgaatc gga
//
LOCUS       DATAOZ010000146         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7163126, whole genome shotgun sequence.
ACCESSION   DATAOZ010000146 DATAOZ010000000
VERSION     DATAOZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7163126"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VHR64_04050"
     CDS             <1..419
                     /locus_tag="VHR64_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzed the formation of 2-ketoglutarate from
                     2-hydroxyglutarate; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX3302029.1"
                     /translation="GPNAVLAFSREGYRFRDVNLRDMAQLFTSPGFLKFGRKHWRTGV
                     DEIARDLSKRRFLASLQRYVPDLTEDHLVPGPSGIRAQALTTSGEMVDDFVIDQQEGI
                     LHVRNAPSPAATSSLQIGKYIVNALAESDDRYRAQA"
     gene            complement(421..1656)
                     /locus_tag="VHR64_04055"
     CDS             complement(421..1656)
                     /locus_tag="VHR64_04055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302030.1"
                     /translation="MLSSTTESRTGLRRHAAVLFILSAGGFTSALNVTLLSPLIVQIA
                     DQFGVSEAAAGQLATLTAACAGLMGILVAPWMDRYSRRFWFRLECSMLATGTILSAIA
                     PEFGWMFLGRILCGLGGAVIGANCLATCGDLFPEPAERNRAIGLVNSAFTLGAVFGLP
                     IVAMTADLTSWRVAIAIPAPLAIAVLIGTRRIPATVRRAEGSIWTAWRAGYRRVIRSR
                     ETVWLLASEILLQVVWFGWLIYFGAFTDNVYGISAALLSLLFFVGGGAEILANNLTPI
                     LTRNRSIAAVAYPAVAIQAINLLMIGIAHNREWSMFPFIAIGSFSGALIFICLNIALL
                     DSLPSDPGAVMSLMSASLELGGSLGVALTGLSLALLDDFETVYRLLGAVAPLIAITFW
                     LSTRNRSASREVVAAPGSV"
BASE COUNT          425 a          723 c          691 g          366 t
ORIGIN      
        1 tgggtccgaa cgcggtgctg gcgttctcgc gcgagggtta tcggttccgg gacgtcaacc
       61 tgcgcgacat ggcccagctc ttcaccagcc cgggattcct gaagttcggc cgaaagcact
      121 ggcgcaccgg tgtcgacgag atcgcgcgcg acctcagcaa gcgccgcttc ttggcatcac
      181 tccagcgcta cgtgcccgat ctgaccgagg atcacctcgt gcccggtccc tccggcatcc
      241 gcgcacaggc cctgaccacc agcggcgaga tggtcgacga cttcgtgatc gatcagcaag
      301 agggcatcct gcatgtccgt aacgcgccgt cgccggccgc gacgtcgtcc ctgcagatcg
      361 gcaagtacat cgtgaacgcc ctggccgagt cggacgatcg ttatcgggcg caggcgtaac
      421 ctagacgctc ccgggcgcag cgacgacctc gcgactggca gatcggttgc gcgtgctgag
      481 ccagaaggtg atggcaatca gcggcgcgac agccccgagc agccggtaga ccgtctcgaa
      541 atcgtcgagc aacgccaggc tcaacccggt caacgccacg ccgagcgagc cgccaagctc
      601 caggctggcc gacatgagtg acataaccgc acccggatcg ctcggcaacg agtcaagcag
      661 ggcaatgttc aggcagatga agatcagcgc cccgctgaag ctgccgatgg cgatgaatgg
      721 gaacatcgac cattcgcggt tgtgcgcaat gccgatcatc agcaggttga tcgcctggat
      781 cgccaccgcc ggatacgcga cggcggcaat cgaccggttc cgggtcagaa tcggtgtcag
      841 attgttggcg aggatctccg ccccgccgcc gacgaagaac agcagggaga gcagcgcggc
      901 cgaaatgccg tagacgttgt cggtgaaggc tccgaagtag atcagccagc cgaaccagac
      961 gacctgcagc aatatctccg acgccagcag ccagaccgtc tcccgcgacc ggatcacccg
     1021 cctatatccc gcacgccacg cggtccagat cgagccctcg gcccggcgca ccgttgcggg
     1081 aatccggcgc gtgccgatca ataccgcgat tgccaatggg gcaggtatgg cgatcgcgac
     1141 ccgccaactg gtaaggtcgg cggtcatcgc gacgatcggc aatccgaaca ccgcaccgag
     1201 cgtaaaagcg ctattgacga gcccgatagc cctgtttcgt tcagcgggct ccgggaagag
     1261 atcgccgcac gtcgcaaggc agttcgcgcc gatcactgcg ccgcccaggc cgcagaggat
     1321 ccggccgagg aacatccagc cgaattccgg cgcgatcgcg gagagaatcg ttcccgtcgc
     1381 caacatgctg cactcgaggc ggaaccagaa tcgccgcgaa taccggtcca tccagggcgc
     1441 gacgagaatc cccatcaggc cggcgcaggc cgcggttaac gttgccagct gtcccgccgc
     1501 ggcctcgctg accccgaact ggtcggcgat ttggacgatc agcgggctca ggagcgtcac
     1561 attcagcgcg ctcgtgaatc cgccggcaga caggatgaac aggaccgcgg cgtggcggcg
     1621 gaggcccgtc cgggattccg tggtcgaact caacatcgtg cttctcgctg ggggctccct
     1681 atggacgcaa cgtgccccat tgcgactggg acggcgcgaa caacaatgac cgcactgaag
     1741 gacgaagctg catgaggtcg gagactgccg aattatgccg ccggtttttc gggtgtcaag
     1801 tccactgttt ttcgtagccg agcgtcggat gatcagaacg acccgctccg taccatacga
     1861 ccggatccgg gcggttggcg cccggattct gggtagaagg ggagtgcgca aatgcgaatc
     1921 ggtggtgggt tgctggcctt gatcgtcatc attatcttgc tgatcttgtt gttctagcag
     1981 accgtctttc cgcaaacact agagtgcggc agcggggttt cctgcgcgct tcatctgtcc
     2041 tggaacgccc gttccccctt tggtacggcg gcattgcgcc ggtcggtcca cgcaggttcc
     2101 ctcgtgcgcc ggccggccaa ccgcatctgt tctgatccag gcccgtgata ccatcagccg
     2161 ctttgcgagt tagccgtcaa ccgccggcca agtgccgcca accgc
//
LOCUS       DATAOZ010000147         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3742936, whole genome shotgun sequence.
ACCESSION   DATAOZ010000147 DATAOZ010000000
VERSION     DATAOZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3742936"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VHR64_04060"
     CDS             <1..990
                     /locus_tag="VHR64_04060"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX3302031.1"
                     /translation="YGRGGAQPTVTDADLFLGYLNPDYFLGGSMELDFAGAEAALADL
                     GTHIDKNALETAWGIHAVVNEQMASAARMHAVEQGKDPRNYPMYAFGGAGPVHAFRVA
                     EILGCPEVIIPYGAGVGSTIGFLVAPVAFDFVRSYVCRLTSADWGIVNQRLDEMESEG
                     RAILEKAGVPADRITFERSAELRYVGQGHDVRVSLPDGHLDESRRPEIEAEFAREYTR
                     LYGRTPTGNPVEAMTWRLIARADDESLGATFSAGQAPGTGEISQTSRAIYLPEDQAMV
                     EVPVYSRYSLPIGFRCDGPCVIEERESTTIVGRHAGVQIDPYRSIVITMRARD"
     gene            complement(1072..1953)
                     /locus_tag="VHR64_04065"
     CDS             complement(1072..1953)
                     /locus_tag="VHR64_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302032.1"
                     /translation="MAVASAFAVCVATGITMTGGGVFVARTGVFVGGTAVFVGGTAVF
                     VGGTAVFVGGTGVSVGAGVLVGGTAVWVAVAVGGTGVWVGVGGTAVSVGFGVLVAVGG
                     TGVSVGLGVSVGFGVLVSVGAGVCVAVGGTAVFVLVGFGVAVSVGLGVCVAVGAGVFV
                     SVGLGVFVAFGVSVGFGVLVAFGVSVGSAVCVAVGFGVSVGTAVCVSVGSTVGEGATV
                     LVAATVLVGAGVVATGAAMVWAAVVLVASPPLLPLSRPQAPRASNAMTATTANRATDT
                     RYAVLIDLFPQLSAYCA"
     gene            2164..3834
                     /locus_tag="VHR64_04070"
     CDS             2164..3834
                     /locus_tag="VHR64_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX3302033.1"
                     /translation="MQFDAIQLEVMWNRLIAVVNEQAKALMRTSFTSIVREAGDLSAG
                     VFDERGYMIAQAVTGTPGHINAMATCIHHFLAEYPADTLAPGDVLITNDPQKTSGHLH
                     DITVITPVFLADRLVGFFGNCCHVLDIGGIGLSTDGRSMFEEGLYIPITKLYDQGTPN
                     TELLKILATNVRTPEPVLGDIHAQVAGNDVGAARLVEFLTEFGLTSLVELGDEIISRS
                     ERAMRNAIAQLPDTVLEHEVVADGYEEPVRLHVTIEKHGDELHVDWAGSSDASMRGIN
                     VVLNYTHAYTTYALKCALAPDVPNNEGSFRPVHVTAPPGCILHALPPAPVAARHILGH
                     FLPGVVFGALAEVIPGRVLAEGAANIWNLQFTGYDLDNNPFTYVWFSCGGTGARPTKD
                     GLHATAFPSGIAGVPAEVIEQLSPVVMWERSIRPDSGGPGRFRGGCGQTLRLGVRTNR
                     PYRFAPLMDRIHHAPKGYDGGMDGAKATVVFSTGEVITRKGTRDMAADTEITLNLPGG
                     GGLGDPADRDPALIERDIRYQLVSPAAAERDYGMAVTADGAVPRMVGV"
     gene            3827..>4050
                     /locus_tag="VHR64_04075"
     CDS             3827..>4050
                     /locus_tag="VHR64_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302034.1"
                     /translation="MSDRRSVELIEVGPRDGLQNESTPVSLADKLTLIARLIDAGFRR
                     IEATSFVSPKAIPQLADADELVPALPDRPDV"
BASE COUNT          808 a         1373 c         1249 g          620 t
ORIGIN      
        1 tacggacgcg gcggggcaca gccgaccgtg accgacgccg acctgtttct cggctacctg
       61 aaccccgact atttcctcgg cggcagcatg gagctggatt ttgccggcgc cgaggcagcg
      121 ctggccgatc tgggtacgca catcgacaaa aacgcgctcg agacggcctg gggcatccac
      181 gccgtcgtca acgagcagat ggcctcggcg gcgcggatgc acgccgtcga gcagggcaag
      241 gacccacgca actacccgat gtacgcattc ggcggggcgg gtccggtcca tgccttccgc
      301 gtcgccgaga tccttggctg tccggaagtg atcatcccgt acggagcggg agtcggcagc
      361 acgatcgggt tcctggtcgc gccggtggca ttcgacttcg tccgctcgta cgtctgccgg
      421 ctgacgagcg ccgactgggg catcgtcaat caacggctcg acgaaatgga gagcgagggc
      481 cgcgcgattc tcgaaaaagc gggtgtgcca gccgatcgga tcacctttga gcgcagcgcg
      541 gagctgcggt acgtcggcca gggacatgat gtgcgcgtgt cgttgccaga cggtcacctt
      601 gacgagtcgc gtcgtccgga aatcgaggct gagttcgccc gcgagtacac ccggctctac
      661 ggtcggacgc cgaccggcaa cccggtcgag gccatgacgt ggcggctgat cgcacgggcg
      721 gacgacgaga gcctcggcgc aaccttctcg gccggacaag cacccggcac cggcgagatt
      781 tcgcaaacgt cgcgggctat ctacctgccc gaggaccagg caatggtcga ggtgccggtt
      841 tactcacgtt actccctgcc gattggattc aggtgcgatg gcccctgcgt gatcgaggag
      901 cgggagtcga cgaccatcgt cggacgacac gctggagttc agatcgatcc atatcgaagc
      961 atcgtcatca cgatgcgggc gcgcgattag gcacagcagg tttgtctatt tgggacaacc
     1021 tgcgtaggat gagcacaaca cgttcaattg aaacggcata ccttgcatcg gtcaggcgca
     1081 ataggctgaa agctggggga aaaggtcgat gaggactgcg taccgcgtat ctgtcgctcg
     1141 gtttgccgtc gttgctgtca ttgcattgct cgctcttggg gcctgcggtc gagacaatgg
     1201 caacaagggc ggcgaagcaa ccaacacgac cgcagcccag accatcgccg cgccagtagc
     1261 cacaacccca gccccgacca atactgtcgc cgcaaccaat acggttgctc cctcaccgac
     1321 ggtcgagccg accgagacac acaccgcggt cccaaccgag actccgaacc cgaccgcgac
     1381 gcacacggcc gagccgacgg agacgccgaa ggcgaccaac acaccgaaac cgactgagac
     1441 gccgaaggca acgaacacgc ccaagccgac ggaaacgaat accccggcgc caacggccac
     1501 gcacactccc aagccgacgg agacggccac gccgaagccg acgagcacga acacagcagt
     1561 gccgcccacg gcaacacata cccctgcccc aacggaaacc aatactccga agccgacgga
     1621 gacgcccaag ccgaccgaca cgccggttcc accgacggca accaatacgc cgaaaccgac
     1681 ggatacagcg gtgccaccga cgccgaccca cacgccggtg ccaccaacgg ccacagcgac
     1741 ccataccgcc gtaccgccga ccaatacccc ggcgccgacg gacacgccgg taccaccgac
     1801 gaacaccgca gttccgccga cgaataccgc cgtgcctcca acaaacactg ccgtaccgcc
     1861 aacgaataca ccggtccggg cgacgaatac tccgccgccg gtcatcgtga ttccggtcgc
     1921 aacgcagacg gcgaatgcgg aggccaccgc cacgtgcgcg gcgctcctga ctccaacggc
     1981 cacggcgact gccagtccga ccgtaggtga aggcacggca actgcgacgc cgactgcgac
     2041 cgcaacggca acgcctgagg gctcaccgga agcaagtccg cagatcacgc cgacgatcat
     2101 ctgcaagtag gcggataatg gaggggcgca attggcgccc ctccacccta ttgaggtcac
     2161 ccgatgcagt tcgatgcgat ccagctggaa gtgatgtgga accgcctgat cgcggtagtc
     2221 aacgagcagg ccaaggcgct catgcggacg agcttcacca gcattgttcg cgaggccggc
     2281 gatctctcgg ccggcgtctt cgacgagcgc ggctacatga tcgcccaagc ggtcaccggc
     2341 actcccggcc acatcaacgc catggcgacc tgcatccacc acttcctggc cgagtacccg
     2401 gccgacacgc tcgctccggg cgacgttctg atcaccaacg atccgcagaa gacatcgggg
     2461 cacctgcacg acatcacggt gattacgccc gtcttcctgg cggatcgact ggttggcttc
     2521 ttcggcaact gctgccacgt cctcgatatc ggcgggatcg gcctctcgac cgacggccgc
     2581 tcgatgttcg aagagggcct ctacatcccg atcaccaagc tctacgatca gggcacgccg
     2641 aacacggagc tgctcaaaat cctcgcgacc aatgtgcgga ctcccgaacc cgtgctcggc
     2701 gatattcatg cccaggtcgc tggcaacgac gttggcgcgg cgcggctggt cgagtttctg
     2761 acggagttcg gtctgacgtc gctcgtcgag ctcggggatg agatcatctc acgctctgag
     2821 cgcgcgatga ggaacgcgat cgcgcagctg ccggatacgg tcctcgaaca tgaggtcgtg
     2881 gccgatgggt acgaagagcc ggtccggctc cacgttacga tcgagaagca cggcgacgag
     2941 ctccacgtcg attgggctgg aagctcggat gccagcatgc gcgggatcaa cgtcgtcctg
     3001 aactacacgc acgcctacac gacctacgcg ctgaaatgcg cgctggcgcc ggatgtcccg
     3061 aacaacgaag gcagcttccg gccggtacat gtcaccgcgc ctcccggctg catcctccac
     3121 gcgttgccgc cggcgccagt cgcggcgcgc cacatcctcg gccatttcct gcccggtgtc
     3181 gtcttcggcg cgctcgccga ggtaattcct ggccgggtcc tggctgaggg cgcggccaat
     3241 atctggaacc tccagttcac agggtatgac ctcgacaaca acccgttcac ctacgtctgg
     3301 ttctcctgtg gtggtaccgg tgcgaggccg accaaggatg gcctgcacgc gacggccttc
     3361 ccaagcggga tcgccggcgt acccgccgag gtgatcgagc agctctcgcc ggtcgtgatg
     3421 tgggagcggt cgatccggcc cgattcgggc ggcccgggac ggttccgggg tggctgcggc
     3481 cagacgttgc ggctcggcgt ccgtacaaat cgcccgtatc gattcgcgcc gctgatggat
     3541 cgcatccatc acgcgcccaa gggctacgac ggcgggatgg acggagcgaa agcaacggtc
     3601 gtcttctcaa ccggcgaggt catcacccgg aagggcacac gggacatggc cgccgacacc
     3661 gagatcacgc tgaaccttcc gggtggcggt ggactgggcg acccggcaga tcgcgatccc
     3721 gccctgatcg agcgcgacat ccgataccaa ctcgtcagcc ccgcggctgc cgaacgagat
     3781 tacggcatgg ccgtcactgc cgacggcgcc gtaccgagga tggtcggtgt ctgatcgacg
     3841 ctcggttgag ctgatcgagg tcggaccacg ggacggtctg caaaacgagt cgactcccgt
     3901 ttcacttgcc gacaaactga ccctcatcgc gcggttaatt gacgccggtt tccgccggat
     3961 cgaggcgacc agcttcgtca gtccaaaggc gatcccgcaa cttgccgatg cggatgagct
     4021 ggtgccagcc ctgcctgatc gtccggatgt
//
LOCUS       DATAOZ010000148         4817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9267055, whole genome shotgun sequence.
ACCESSION   DATAOZ010000148 DATAOZ010000000
VERSION     DATAOZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9267055"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(800..3259)
                     /locus_tag="VHR64_04080"
     CDS             complement(800..3259)
                     /locus_tag="VHR64_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3302035.1"
                     /translation="MANAASNQSTGQTDPFAARLRQSRERAGLSQEELADRAGVSANA
                     ISALERGVRQRPYPATIRALVDALELTGEERAAFIASGRNNVAEPEESPASVTTLPTP
                     RTSLIGREQDVARVCELLRRADVSLLTLTGPGGVGKTRLALRVAEEMADEFSGGVFFL
                     PLAPIHDPALVGPTIAKVLGVRDVGDQPLARRIAAKLSATYPLLVLDNLEHLLAAASF
                     LADLLASWPNLSVLVTSRERLRIGDEREFPVDPLPLPSERTPVPIWALSENPAVRLFV
                     ERAAAIDPTFALDDENAGSVAEIVRQLDGLPLAIELAAARVRILPPPALLARLAKRLP
                     LLVGGNRDAPARQQTLRDTIAWSHQLLTPAEQELFRRLAVFVGGFTLEAAEAVAGPAT
                     GGAIFDGIAALVDHNMVRPAGRTGDEPRFMMLETIREFGLECLGEAGEEAAIRDAHAA
                     WFLEFVERADCEVVRFLPLGDQIFERLEADHANLRAALIWLEQTNPTGMLRLAGGMGP
                     FWGTRGYVREGQAWIERALAAAGDASRHARALGLFELALVTNLRGNEHDAMRLNLESL
                     AIWRELEDARGIVLALQRAGLIALRRNEFDLAVAMQTEALVTLETIRDEPWAACLASS
                     LHGHIGNIAIARGDLDQAESHFSEAIRRQTALGYEPGTSHIYASHPVAGLGDVCRGRN
                     DSARALGWYQQSLASAWQFRDMRAVAYAMSGVAASLAATGQWQSAARLFGAAEAIHDA
                     SGLAFILESMDRQRALGLPEPWLRATESFGIGQRLREALQSQGQISHAPIPDPEAANR
                     LWSLGRRLPPEEAIAEALAAG"
     gene            3432..3881
                     /locus_tag="VHR64_04085"
     CDS             3432..3881
                     /locus_tag="VHR64_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302036.1"
                     /translation="MNTAVRWAATLVLFMLISGLQLHLNDAAATPGPVNPGRLSPFVA
                     ECANTDDAGQHGADIVVMGPGGDFAGATVIHVAAAKPASTGILVLGPGGDFAGATVAE
                     PTSAELVATDHVVFGPGGDFAGATVIHVDMPAASDGLATDGTQSTCG"
     gene            4192..4695
                     /locus_tag="VHR64_04090"
     CDS             4192..4695
                     /locus_tag="VHR64_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302037.1"
                     /translation="MSDLGVPRSDAASHPAGARAALRVSEQAVERIDKPSARLQPRSR
                     VAFNKQAWWAVLLVAVGVISIATALGFQRVHNNNTVVVFGQVTEQAPMAWYYDHPWFG
                     ALVLAGLPALAFAWLIVTLLETRHRAAELQTDVADLTLQLQHIGYRLQGQLDQIAARL
                     GEEAPTP"
BASE COUNT          832 a         1657 c         1517 g          811 t
ORIGIN      
        1 agaacctaaa acccaaaacc caaaaccacc cgagtatttc tactcacctc gccgttcatt
       61 ccgaatcgcg taattcttac gatgcggagc cgcctcttgc cgcattacgg tgtctgtcag
      121 tcataaaggt gaatagtcca ggcgtcaggc gatctgcgct gcgtcgatga gccaagggtt
      181 gggcgacgcg gtgaaagccg tgagccaggc gagatgctca agcgtcacct cccccggggc
      241 aggtcgactg caccctgaac gtttgcctgt tgaccatcga cagcggcgct ggggccgctc
      301 tctccccctc ccccggtgga gtcccggcgc cgcatctccc aaacccagga gacgacgatg
      361 cattcgccct atcacgccat acccgcatcg ccgtcgcatt cacatcgtag gtccgcttgt
      421 gacaagcctg ggccggatca cggtcccggg gaggtgtgtg aaaggagata catcatgcga
      481 agaaccctgc atcctcggct gatctgacaa cgcgggacgg caacctggtc cagcgatcag
      541 gctgccgggt ccggacagag cacgttagcc gttcgatgtt gatggttggt tgttggtggt
      601 ggggcttggt ccaccgtttt cggcaattcg gtatccgtga tctcccaccc ctccacccgc
      661 aacccgccac ctcacgctac atctcggtac ggtacgtgca atggcgccgc cgagagagcc
      721 aagatcgtat ggcattgcga cgctaccctt cgttcccaca acccacaacc cacaacccac
      781 aaccctcaac cctccaccct caacccgctg cgagtgcctc cgcgatcgcc tcttcaggcg
      841 gcaggcggcg gccgagcgac cagagccgat tcgcagcctc cggatcaggt atcggcgcat
      901 gtgagatctg cccctgggac tgcaacgctt cccgtaaccg ctgcccgatg ccgaacgact
      961 cagtcgcccg cagccacggc tccggcaagc cgagggcccg ctgccgatcc atcgattcca
     1021 ggatgaaggc gaggccgctc gcgtcgtgga tcgcctctgc agcgccgaac aatcgcgccg
     1081 cggactgcca ttgcccggtt gccgccaacg atgccgcgac gccactcatg gcatacgcca
     1141 ccgcccgcat gtcccggaat tgccacgcgc ttgccagcga ctgctgatac cacccgagcg
     1201 ctcgggcaga atcgttgcgg ccgcggcaca catcgcccag tcccgcgacc ggatgactgg
     1261 catagatatg gctggtgccg ggttcgtagc cgagcgctgt ttgccgcctg atggcctcgc
     1321 tgaaatggga ctctgcctgg tccagatctc cacgcgcgat ggcgatattg ccgatgtggc
     1381 catggagcga gctggcgaga cacgcggccc agggctcgtc gcgaatcgtc tccagggtca
     1441 caagtgcctc ggtttgcatc gccaccgcga gatcgaactc gttgcgccgg agtgcgatga
     1501 ggccagcccg ctgcagcgcc agaacaatgc cccgggcgtc ctcgagctcc cgccaaatgg
     1561 ccaggctttc gagattgagc ctcatcgcgt cgtgctcgtt gccgcgcagg ttggtcacca
     1621 atgcgagctc gaacagaccc agcgctcggg cgtgccgcga cgcgtcgccg gcggccgcca
     1681 gcgcgcgctc aatccatgcc tgcccttcgc gaacgtaccc gcgggtgccc cagaatgggc
     1741 ccatccctcc cgccagccgg agcatcccgg ttgggttggt ctgctccaac cagataaggg
     1801 ccgcccgcag gttggcgtga tcagcttcca ggcgctcaaa tatctggtcc cccagaggca
     1861 ggaagcggac cacctcgcaa tcggcccgtt cgacgaactc cagaaaccag gcagcatggg
     1921 catcccggat tgccgcctct tcaccggcct cgccgagaca ttccaggccg aattcccgga
     1981 tcgtctcgag catcatgaag cgcggctcat ctccggtgcg gccggccggc cgcaccatgt
     2041 tgtgatccac cagggcggca atgccgtcga agatggctcc cccggtcgcg ggacctgcca
     2101 cggcttcggc tgcctccagc gtgaagccac ccacaaagac ggcgagccgg cggaagagtt
     2161 cctgttcggc cggggtcagc aactgatggg accatgcgat cgtgtcccgc agggtctgct
     2221 gccgggcggg cgcatcgcga ttcccgccga ccagcagcgg cagccgcttc gccaggcgcg
     2281 ccagcaatgc cggcggcggc agaatcctga ctctggccgc cgccagctcg atcgccagcg
     2341 gcagcccgtc cagctgacgc acgatctcgg ccaccgagcc ggcattctcg tcgtccagcg
     2401 cgaacgtggg atcgatggcg gcggcgcgtt cgacgaacag gcggacggcc gggttctcgc
     2461 tgagcgccca gatcgggacc ggcgtgcgct cactgggcag cggcaacgga tcgaccggga
     2521 actcccgctc atcgccaatg cgcaatcgtt cccggctcgt gaccaggacc gagagatttg
     2581 gccaactcgc caagaggtcg gccaggaacg atgcggccgc aagcaggtgc tcgaggttgt
     2641 cgaggacgag cagcggatag gtcgcgctga gcttggccgc aatccgccgg gccagtggct
     2701 ggtcgccgac gtcgcgcacg ccaagcacct tggcgatcgt gggtccgacg agggcggggt
     2761 catgaattgg tgcaagcggg aggaagaaga ctccgccgct gaattcgtcc gccatctcct
     2821 cggcgacgcg caacgccagc cgggtcttgc cgactccgcc cgggccggtc aaggtcagaa
     2881 gcgaaacgtc ggcccggcgg agtagttcgc agactcgagc aacatcctgc tctcggccga
     2941 tcagcgaggt tcgcggcgtc ggcaatgtcg ttacgctggc agggctttcc tcgggctcgg
     3001 ccacattgtt ccgacccgat gcgataaacg ctgcccgctc ctctccggtc aactccagcg
     3061 catcgaccag agcccggatc gtggcgggat acggccgctg gcgcacgccc cgctcgagcg
     3121 cgctgatcgc gtttgcgctg acgccggccc gatccgccag ctcctcctgg gacaacccgg
     3181 cccgttcccg cgattgccgt aatcgggcgg cgaacgggtc ggtctggccg gtcgattggt
     3241 tcgatgcggc gttggccatg gtccctcgct ggagctggaa aggatcgtca cctgacaggg
     3301 taatcccttc ggcaagtgaa agtgtgcgag gagtgtgcga cccgtgtccg tggtgacctg
     3361 gcaacgctgg gcagatgctg gctgcgcggc ccgatacgcg ccgcgaccgg caaagggata
     3421 aggagcgcgc tatgaatacg gcagttcgtt gggcggcgac cctggttctg ttcatgctga
     3481 tatccggact tcaattgcac ctgaacgatg cggctgcgac gcccggtccg gtgaaccccg
     3541 gtcggttgtc tccattcgtg gctgaatgcg cgaataccga tgacgccggc caacatggcg
     3601 ctgacatcgt ggtgatgggg cccggcgggg acttcgcggg agcgacggtg atccatgttg
     3661 cggccgccaa gccggccagc acgggcatcc tggtgctcgg tccgggcggc gacttcgccg
     3721 gcgcgacggt cgctgagccg acaagcgctg aactggtcgc caccgatcat gtcgtgttcg
     3781 gtccgggcgg tgatttcgcc ggagcaaccg tgatccacgt cgacatgccg gcggcatcgg
     3841 acggcctcgc cacagacggc acccagtcca cgtgcggctg atgcccaatc ggggcagcag
     3901 cccacatcca gaatccccca tcgcaatctg aaacacagcc cgcttcggcg ggctttcttc
     3961 ctggagctca atgcatgacg ccgccagcac tagccaccga tctatcgggt aacgcaaggc
     4021 atgtacatcc cctctccggc tctccccacg gggaagagag catccacgcc actacgtcgg
     4081 ctagacgatt cggcccgggc gacttcagtt gtcgcaccgg atgcgatcgg gattgggcag
     4141 gctatggtat ctagaggtgt cggatgggtt gctccggcag aggaggattc gatgtctgat
     4201 ctcggcgtgc cccgcagcga cgcggccagt catcccgctg gtgcgcgggc agcgctgcgc
     4261 gtctcggagc aggcggtcga gcggatcgac aagccgtcgg cgcggctgca gcctcgctca
     4321 cgggtcgcgt tcaataagca ggcctggtgg gcagtcctgc tcgtcgccgt gggagtgatc
     4381 tccatcgcta cggccctcgg cttccagcgg gtccacaata acaacacggt cgtcgtcttc
     4441 ggccaggtga ccgagcaggc ccccatggcg tggtattacg accacccgtg gttcggcgcg
     4501 ctcgtcctcg ccggtctccc ggcccttgcg ttcgcctggc tgatcgtgac gctcctggaa
     4561 acgcgccacc gcgccgccga gctccagacc gacgtcgccg acctcacgct ccagctccag
     4621 cacatcggct accgcctgca gggccagctc gaccagatcg ccgcccgcct cggggaagaa
     4681 gcaccgacgc cctgaacccg ggcgtcccgc gtcgtaaccc ggccgattac cagacgacga
     4741 tgcgttccgc gggcgggaga tacatcggat ctcccggccc aatcgcgaat gcgtcgtaga
     4801 actcgtccat gttccgg
//
LOCUS       DATAOZ010000149         5300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6899447, whole genome shotgun sequence.
ACCESSION   DATAOZ010000149 DATAOZ010000000
VERSION     DATAOZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6899447"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1775)
                     /locus_tag="VHR64_04095"
     CDS             complement(105..1775)
                     /locus_tag="VHR64_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3302038.1"
                     /translation="MTAAQLSRRRFMQSLLASGALAAGAGVLRPGGVFAQDATPAATP
                     IPPFSGELAADQEMRLPITEPTTMDPGVSYGDDELSIFYNIFDGLIGIDQQTGEVVPR
                     VAESYEANADASEYTFHIRQGVTWSDGTPLTSQDFLYSWKRVLDPNTLSQYIPALYPI
                     KNAEKIANGEADISEFGVAAPDDSTLVVTLEGPTTFFPLLATTWTYFPVPKHVIDEKA
                     EAWVEAENIVSNGPYKMTEWSHNQQIVLERNDSYYGDKPTITKATYRLYEDDTLQAYT
                     SFENNELDYAEPGGPDLERILADPTLSAELIQFPLSNTYFIIGDTTNPPTDKVEFRQA
                     LNKSVDRETLASTVFKGEYLPAASMLPPNIPGNNPAAALPESVDEAKQLLSSAGIDPS
                     SINIELAYRNVSPYKTLAEFLQSSWQEALGIAIKLTPIEDNAYSDWRAARAETKFNLY
                     TGVWGSDFADASNWFNQNFTTSADHYQAHWSNAEFDQLVETAITNTNTEERNEQYSQA
                     EVILVRESPVIPVMRGKAFRAVKPWVKDIYFQPLLSVVHLRTVKIAAH"
     gene            complement(2420..4171)
                     /locus_tag="VHR64_04100"
     CDS             complement(2420..4171)
                     /locus_tag="VHR64_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX3302039.1"
                     /translation="MAETVGDVLIERLIDWGVDTVFGLPGDGINGVFEGLRTRQDKIR
                     FIQVRHEEAAAFAACGYAKYTGRLGVCIATSGPGGIHLLNGLYDAKCDGAPVLAITGH
                     TYHDLTGTHYQQDVDLAKLFVDVAAYNERVMGPTHVENVVDQAIRTALTERTVAHITI
                     PKDFQEWSANGHRSMANIPGHSANHFAAAHPLPATEELQRAADLINGGKKVAILAGRG
                     CLNARVEILDLAEKAAAPIVKALLGKATVPDDSPYTTGGIGLLGTAPSQDALTECDTL
                     IIAGSSFPYIESYPKPGQAKTVQIDIDPERIGLRHPVEAGLIGDCGAVLRALLPLIER
                     KEDRGFLEKAQERMSDWRALMEERGTRGDLPMKPQVVAYALNKLLADDAIIAADSGTI
                     TTWSARYLDIRGDMHYSLSGSLATMASGLPYAIGAAVAYPNRQVIAFVGDGGFTMLMG
                     EVATLVKYGLPVKVIVIKNNLLGQIKWEQMVMNANPEFGVELQPIDFATFATACGAAG
                     YTIEHPNDAEPVLREALAQPGPAVIQAVVDPNEPPMPGKITTEQAVNFAKSLAKGEKD
                     RFEILKTVLKDKVREVV"
     gene            4646..>5300
                     /locus_tag="VHR64_04105"
     CDS             4646..>5300
                     /locus_tag="VHR64_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3302040.1"
                     /translation="MLEIDHRCGGWMSVRYSSGFLSGRVAIVTGAGSGIGRAVAHRLA
                     GAGASVIVAEADSESGADAAASITAAGGQARFVPCDVAGSANVQTMIAETLRSLGRLD
                     ILVNNAGIPGPSASADRYPDDDWDRVIAVNLGGVFRCSKYAIPHLEASGRGAIINIAS
                     TFGMVGAPESPAYCAAKGGVINLTRQLAVDLGSRGIRVNAVCPGYVDNDMARRGDQLP
                     "
BASE COUNT          959 a         1571 c         1670 g         1100 t
ORIGIN      
        1 agtcccggag ggacttcgtc gtcttagctc cgggttgaac cccggggaac tccattgaga
       61 gccgggcaac agaaattggc gctacccggc cctcaagaga acctctagtg cgccgcgatc
      121 ttgactgtac gcaggtgcac caccgagagc agcggctgga agtagatgtc cttaacccac
      181 ggcttgactg cacggaacgc cttgccgcgc atcaccggaa tgaccggtga ttcccggacc
      241 aggatcacct cggcctgcga atactgctcg ttgcgctctt cggtgttcgt gttcgtgatc
      301 gccgtttcga ccaactgatc gaattccgcg ttcgaccagt gcgcctgata gtggtcggcg
      361 gaagtggtaa agttctggtt gaaccagttc gacgcgtcgg cgaagtccga accccagacg
      421 ccggtgtaga gattgaactt cgtctccgcg cgggcggcgc gccagtcgct ataggcgttg
      481 tcctcgatcg gcgtcagctt gatcgcgatg ccgagcgctt cctgccagga cgactggagg
      541 aattccgcca gcgttttgta gggcgagacg ttgcgatagg cgagctcaat attgatcgac
      601 gagggatcga ttccggcgct cgacaacagt tgcttcgcct cgtccacgct ctccgggagc
      661 gcggccgcgg ggttgttgcc cgggatgttc ggcgggagca tcgacgccgc cggcagatac
      721 tcacccttga agacggtgct ggccagcgtc tcgcggtcga ccgacttgtt gagtgcctgg
      781 cggaactcga ccttgtcggt cggtgggttc gtcgtgtcgc cgatgatgaa gtacgtgttc
      841 gagagtggga actggatcag ctcagctgag agtgtgggat cggccaggat gcgctcgagg
      901 tctggtccgc ccggctcagc gtaatcgagc tcgttgttct cgaacgaggt ataggcctga
      961 agggtgtcat cttcgtacaa gcggtacgtg gccttggtga tcgtcggctt gtcgccgtaa
     1021 tacgaatcgt ttctctccag cacgatttgc tggttgtgcg accactcggt catcttgtac
     1081 ggaccgttgg agacgatgtt ctccgcttca acccatgcct cagccttctc gtcgatcacg
     1141 tgcttcggca cggggaagta ggtccaggtg gtggcgagaa gcgggaagaa cgtcgtcggg
     1201 ccttcgaggg tgacgacgag ggtcgaatcg tccggcgcgg ccacgccaaa ctccgagata
     1261 tcggcttcgc cgttggcgat cttctcagca ttcttgattg gatagagcgc ggggatgtac
     1321 tgcgagaggg tgttcggatc gagcacgcgc ttccagctat agaggaagtc ttgcgaggtg
     1381 agcggtgtgc cgtccgacca ggtcacaccc tggcggatat ggaacgtata ctcggacgca
     1441 tcggcgttcg cctcgtaact ctccgctacc cgcggcacga cctctccggt ctgctggtcg
     1501 ataccgatga ggccatcgaa gatgttgtag aagatagaga gctcgtcgtc gccgtagcta
     1561 acgccggggt ccatcgtcgt cggctcagtg atcggcagcc tcatctcctg atcggcggcc
     1621 agctcgccac taaatggcgg gatcggcgtt gcggccggcg tcgcgtcttg cgcaaagacc
     1681 ccgcccggcc ggaggacgcc ggcgcccgcg gccagcgcgc cggacgcgag gagactttgc
     1741 atgaaccggc ggcggctcaa ctgagctgcc gtcaacttct ttccatttgc gaggaatacg
     1801 tccgctagtt tttcgcccga tgtcacgatt tccccctttg tacgaattgg tctcgccgga
     1861 acgcttcgag cgaaaccgca ttcaatgcga caggtatgaa cacggaagac acatgcaaca
     1921 acggtccgtc agcaaagcgc cggaccagat cgcgatcaat gctgggaatg gaacctcctc
     1981 aatacaccta cgcggggtgc ccatcgccag cgctgtgtga tcgtgtctcg gctgcattgt
     2041 cgaactgtat cggcggagga taacaatgcg gtgcctctcc gtcaaacgag caggagatag
     2101 gcgtcaggcg tcaggcgtca ggcgtcgggc gtcaggcgtc aggcgtcagg cgtcaggcat
     2161 cgggcgttag gtgggagcgt cggcattgcg cgcacgccct cccgtcattc cgcgcccacc
     2221 gctctcgtcc tttcgatgcc gccctccccg tcatttcgag cgcccacccc gcccgtcatt
     2281 tcgagcgaag tcgagaaatc tgtcctgtcc agcaggacag acccggccag gcgagatcct
     2341 gagcgaagca aaggatgacg ggagagcaac gctcagaatg acgagccggg gcgcacgttg
     2401 ctttctttgg aacgacccgc tagaccacct cgcgcacttt atctttgagc acggttttca
     2461 gaatctcgaa gcggtccttc tctcccttgg ccagagattt cgcgaagttg actgcttgct
     2521 cggtcgtgat cttgcccggc atcggcggct cgttcgggtc gacgactgcc tgaataacgg
     2581 ctggtcccgg ctgagccagc gcctcgcgca aaacgggctc ggcatcgttg ggatgctcaa
     2641 tcgtgtagcc ggccgcgccg cacgccgtgg cgaaggtggc gaagtcgatt ggttggagct
     2701 cgacgccaaa ctccgggttg gcgttcatca ccatttgttc ccacttgatc tgaccgagga
     2761 ggttgttctt gatgacgatc accttgaccg gcaacccgta cttgacaagg gtcgcaacct
     2821 cgcccatcag catcgtgaaa ccgccatctc cgacgaaggc gatcacctgc cggtttggat
     2881 acgcgaccgc tgccccgatg gcgtaaggca gcccgcttgc catcgtcgcc agtgagcctg
     2941 agagcgaata gtgcatgtcg ccgcggatgt cgagataacg ggccgaccag gttgtgatcg
     3001 tgccgctgtc ggcggcgatg atggcatcat cggccaagag cttattcagc gcatacgcga
     3061 cgacctgggg cttcattggt aggtcgccgc gagtacctcg ctcctccatc agcgcccgcc
     3121 aatcagacat ccgttcctgc gccttttcca gaaagccgcg atcctctttg cgctcgatca
     3181 gcgggagaag cgcgcgcagc acggcgccac aatcaccgat gagcccggcc tcgaccgggt
     3241 gacgcaatcc gatccgttcg ggatcgatgt cgatctgtac agtcttcgcc tggccaggtt
     3301 tcggatagga ctcgatataa gggaagctgc tcccggcgat gatgagcgtg tcgcattcgg
     3361 tgagcgcatc ttgcgatggc gcggtgccga ggagaccgat accgccggtt gtgtagggac
     3421 tgtcgtcagg cacggtcgcc ttgccgagca atgccttgac aatcggcgcg gccgctttct
     3481 cggccagatc gagaatctca actcgggcgt tcaagcaacc gcggccggca aggatggcga
     3541 ccttctttcc gccgttgatc aggtcggccg cccgctgaag ctcctccgta gccggcaatg
     3601 gatgggccgc ggcgaaatga ttggcgctat gcccggggat gttggccatt gagcggtggc
     3661 cattggcgct ccactcctgg aagtccttgg gaatggtgat gtgtgcgacg gtccgctcgg
     3721 tcaacgcggt cctgatcgcc tggtcgacga cgttctcgac atgagtcgga cccatcacgc
     3781 gctcgttata cgcggcaacg tcgacgaaga gcttggcgag atcgacgtct tgttgatagt
     3841 gcgtgccggt gaggtcgtgg taggtatgac cagtgatcgc cagcaccgga gcgccatcac
     3901 atttggcgtc atagagtccg ttgaggagat ggatgccgcc cggaccggaa gtggcgatgc
     3961 aaaccccgag gcggccggta tatttggcat agccgcaggc cgcgaaagca gcggcctctt
     4021 catggcggac ctggatgaac cttattttgt cctggcgcgt ccgcagacct tcgaacacac
     4081 cgttgatccc gtcccccggc agcccaaata cggtgtctac accccaatcg atgagacgct
     4141 cgatcaggac atcgccgact gtttccgcca tcttagtctt cctcatctgc gctgtaagcg
     4201 ttgtctcgtc gcagccggcc ggaccggcag ttgacgaaga aggacgccct caacgggcgt
     4261 tgacttcagt tgcgtactgc ctgtcagcgc gtgaactgct cctgccaaac ggagacaatg
     4321 gtcactgttg aacgcttgca gtgcccaatc tcatcgaggg tggaccgtat cacctatgaa
     4381 tccatgtcga ggatgccggc gagatctcgt cgtgagttgt ggtactcatg taggagaagt
     4441 gtcggatcct gtttcgggcc ccggtagcta tcgcctactt cggtgatggc tgcagagagg
     4501 tcctcgcgaa aggtgaaggc gtgctgttct tgcgcagcgc cttgaagtcg aaccgatttc
     4561 gcaagttcga tgccctcttg gatcgttcag cggccgcggc gccgagtacg ctctacacca
     4621 gtcaggaagt cgccagggct aggctgtgct ggaaattgat cacaggtgtg gagggtggat
     4681 gagcgtgagg tattcgagtg gcttcctcag tgggcgtgtc gctatcgtca cgggcgctgg
     4741 ttcaggaatt ggccgcgctg ttgcgcaccg gctcgcggga gcgggcgcat cggtgatcgt
     4801 ggccgaggcg gatagtgaga gtggcgccga tgcggcggct tcgatcaccg cagcgggcgg
     4861 acaagcccgt ttcgttccgt gtgacgttgc cggcagtgcg aatgtgcaaa ccatgatcgc
     4921 ggaaacgctc cgctcgcttg gtcggctcga catcctggtc aacaacgccg gaatacccgg
     4981 tccttcggcc tctgccgatc gctatcctga cgacgattgg gatcgagtga tcgccgtcaa
     5041 tctgggcggc gtattccgct gcagcaaata cgcaatcccg cacctcgaag cgtccggacg
     5101 tggcgcgatc attaatatcg cttcgacctt tggcatggtc ggcgcgcccg aatcgcccgc
     5161 ctactgcgcg gcgaaaggcg gggtgatcaa cttgacgcgc cagcttgccg tcgatctcgg
     5221 ttcgcgcggt attcgtgtca acgccgtctg ccccggttac gttgacaacg acatggcccg
     5281 ccgcggcgac caattaccac
//
LOCUS       DATAOZ010000150         4672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1113799, whole genome shotgun sequence.
ACCESSION   DATAOZ010000150 DATAOZ010000000
VERSION     DATAOZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113799"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VHR64_04110"
     CDS             complement(<1..520)
                     /locus_tag="VHR64_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3302041.1"
                     /translation="MVALRVLLFAIAGLAFALIDDWPAITVISTALMVVIVLSFIWSR
                     TSLTGLSFARRLSSDRAQVGDQLREDLAVRNQSIFGKLFLEIEDRSTLPGHLANRVIK
                     LGPRSARQWRVSSVARERGRHRLGPVTLRSGDPFGLFRRQKKLKETLDLTVYPLTYDL
                     QPYVPGSVLQSGG"
     gene            complement(520..1503)
                     /locus_tag="VHR64_04115"
     CDS             complement(520..1503)
                     /locus_tag="VHR64_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3302042.1"
                     /translation="MQVQRVQELAARVTSNVERVVVGKRREVELVLVALLCRGHVLIE
                     DVPGVGKTVLAKAIARSIGASFKRVQFTPDLLPGDVTGVSVFDPQHVRFEFRPGPVFA
                     QFVLADEINRATPKTQSALLEAMEEAQVTVDGVTHLLPEPFIVLATENPIDYEGTFPL
                     PEAQLDRFLMRISMGYPGRAGELEMLERQQHQHPLELIGQVIDVASLVAAQAEVRDIR
                     VDSAVADYVVSIVESTRNHPDVQLGASPRGSLALYNSSRALAALNGRQFVIPDDVKAL
                     AVATLAHRLIVSPAARVRNVDSRLIVQEIVRGLPVPGARPMAAAGRVQTVT"
     gene            1630..4374
                     /gene="polA"
                     /locus_tag="VHR64_04120"
     CDS             1630..4374
                     /gene="polA"
                     /locus_tag="VHR64_04120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX3302043.1"
                     /translation="MPDKRPQSVMLIDGYGLIFRAYHAIDSAMSTSSGEQTNAVFGFT
                     RMLLDVLESQRPEYAIVALEGGKTFRHDEYDGYKANRGAMPDDLRSQVHRVREIIGAM
                     NIPIIEREGFEADDVIGSLGHKLADDKHLHVLIITGDSDLLQLVQPGVEAVLPGRPRF
                     QDLRIFDVAAVEDRYGFGPELIPDYKALVGDTSDNIPGVPGIGDKTAKSLIQQFGDVE
                     SILANLDSVTPPRAKNALAANIDTLRLSKRLATIVRDLEIDPDLEHSAVDNFDRDKVI
                     DIFRELEFRSLVHRLPESRQPDSKPVPELDRPEPSRTTVTTDDELKAVAKRVKETGEY
                     AFDVETTSLNPLTAELVGLAIAASPTESWYIPVAHGEGSVAPERIAAHLIEMFTDSEI
                     KGYAHHGKYDMHVLTRYGIDVQNLEFDTMIAAYLLGENSVGLKDLAFSRLGIEMTEIT
                     ALIGTGRNQLSMSVVPLDQVAQYACGDVEATFELVNYYRPRLNENNQEWLFRKVEMPL
                     IQVLSEMERMGISIDCEYLAELDKEIRQRLMELDREITRLAGRPINVNSTRQLGALLF
                     DEFKLPSGRRTKTGYSVDQDLLETLRDQHAIIPVILEYKTLLKLSSTYVSALPAEVDP
                     KTGRIHTSYNQTVAATGRLSSNSPNLQNIPIRTEVGRRVRRAFVADHSERRIFEDSVM
                     LSADYSQIELRLMAHMSNEPFLLEAFRGGADIHRATAALVYGIDPADVTPDMRRVAKT
                     VNFGLLYGMQAYGLSRDTGLSRADAQAFIDQYWARLPGVKTYFDETLRRGEELGYVET
                     LYGRRRAVPDLTSPNGQRRAAAVRVATNMPLQGTAADIMKIAMVRLQEQLRDRSDRAV
                     MLLQVHDELVLEVNRPALDEIGALTRQVMEDAAELAVPLVADLSVGPNWDDQSDFDPA
                     "
     gene            4462..>4672
                     /locus_tag="VHR64_04125"
     CDS             4462..>4672
                     /locus_tag="VHR64_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX3302044.1"
                     /translation="MPTYQYQCRDCGHGFEQFQKFSDDPLTVCPSCEGSVQRVIQNVG
                     VVFKGSGWYINDSRKPEKSESGAPAE"
BASE COUNT          947 a         1461 c         1446 g          818 t
ORIGIN      
        1 cgccgcctga ttgcagcacg gaaccaggaa catacggctg caagtcgtat gtcaaggggt
       61 ataccgtcag gtcgagcgtt tccttgagct tcttctgtcg tcggaagagc ccaaagggat
      121 cgcccgagcg caacgtgacc ggcccaaggc ggtgacgacc acgttcccgc gccaccgatg
      181 aaacgcgcca ttggcgagcc gatcggggac cgagcttgat cacccggttc gcgaggtggc
      241 cgggcagcgt gctgcggtcc tctatctcaa ggaagagctt gccgaaaatg ctctggttgc
      301 gaaccgccag atcctcgcgc agttggtcgc cgacctgcgc ccggtctgat gagagccggc
      361 gcgcgaacga taaccccgtg agactcgttc gcgaccagat gaacgagagc acgataacaa
      421 ccatcagggc ggtggaaatc acggtgatcg cgggccagtc gtcaatcagg gcgaatgcga
      481 gcccggcgat cgcgaagagg aggacgcgca gcgcgaccac tacgtgaccg tctgcactcg
      541 cccggcagcg gccattggtc tggcgccggg cacgggtagc ccgcgaacga tctcctgcac
      601 gatcaggcgg ctgtcgacat tgcgaactcg tgcggccggc gagacgatca ggcgatgggc
      661 gagcgtcgca accgcgagcg ccttgacgtc gtcagggatc acgaactggc gaccgttgag
      721 cgctgccaac gcacggctgg agttgtacag cgcgaggctc ccgcgggggc tcgcgccgag
      781 ctggacatcc ggatgattgc gcgttgactc gacaatcgag acgacgtagt cggccacggc
      841 ggagtcgacc cggatatcac ggacttcggc ctgtgccgcg accaggctgg cgacatcgat
      901 gacctggccg atgagctcaa gcggatgctg gtgctgctgg cgctcaagca tctccagctc
      961 gccggcccga cccggatagc ccatcgagat ccgcatcagg aatcggtcga gctgcgcctc
     1021 gggcaaaggg aacgtccctt cgtaatcgat cgggttctcg gtggccagaa caatgaaggg
     1081 ctcaggcagc agatgcgtca cgccgtcgac tgtgacttgc gcttcttcca ttgcttcgag
     1141 cagggcggat tgcgtcttcg gggtcgcgcg gttgatctcg tcggccaaaa cgaactgagc
     1201 gaagaccggt cccgggcgga attcgaagcg aacatgctgc ggatcgaaaa cgctgactcc
     1261 ggtgacatcg cccggcaaaa ggtcaggcgt gaattgcacc cgcttgaacg atgcgccgat
     1321 gctgcgggcg atcgccttgg ccagcacggt cttgccgacc cccggaacgt cctcgatcag
     1381 gacatgtcca cgacagagca acgcgaccag gacaagctcg acttcgcggc gcttgccgac
     1441 taccacacgc tcaacattgc tggttacgcg cgcagccagt tcctgaactc gttgtacctg
     1501 catgaacggg attgctcgat tcacggacgc gtcgaggata ggcaacaaca gtatagcaac
     1561 cgcatcatgc gaacatatga cctacgccgt atactcaacc cagcatttct tgtaggaggg
     1621 gcgacacacg tgccggacaa gcgaccacaa tcggtgatgc tgatcgatgg gtatggcctg
     1681 attttccgcg cctatcacgc gatcgacagc gccatgtcga cttcgtccgg ggaacagacc
     1741 aatgcggtct tcgggttcac ccgaatgctc ctcgacgtgc tcgaatcgca gcgcccggaa
     1801 tacgcaatcg tcgcgctcga gggcggaaaa acgttccggc acgacgagta cgacggctac
     1861 aaggccaatc gcggcgcaat gccggatgat ctccgctcgc aggtccaccg ggtccgcgag
     1921 atcatcggag cgatgaacat cccgatcatc gagcgcgagg gattcgaggc tgacgacgtc
     1981 atcggcagcc tgggccacaa gctggccgac gacaagcacc tgcacgtact gatcatcacg
     2041 ggcgactccg acctccttca gcttgtgcag ccaggtgttg aggctgtgct cccaggccgg
     2101 ccgcgcttcc aggatctgcg gatcttcgat gtggcggcgg tcgaggacag atacggattt
     2161 ggccccgagc taatcccgga ttacaaggcg ctggtcggag acacgtccga caacatcccg
     2221 ggtgtcccag gcatcggcga caagaccgcc aagtcgctca ttcagcagtt cggcgatgtc
     2281 gagtcgatcc tagcgaatct cgattctgtc acccctccgc gggcgaagaa cgcgctcgcc
     2341 gcaaacatcg acacgctccg actcagcaag cgactggcca cgatcgtccg tgatctcgag
     2401 atcgatccgg acctggaaca cagcgcggtc gacaactttg accgggataa ggtcatcgac
     2461 atcttccgcg agctggagtt ccgctcactc gtgcatcgct tgccggaatc ccgccagccc
     2521 gatagcaaac ccgtgccgga gttggaccgg ccagagccaa gccgcacgac cgtcaccacg
     2581 gacgacgaac tgaaggcggt cgcgaaacgc gtcaaagaga ccggcgaata tgcattcgat
     2641 gtcgagacga cgtccctcaa cccgttgacg gccgaactgg tcggtctcgc gatcgccgcc
     2701 tccccgaccg aaagctggta catcccggtc gctcacggcg aagggtcggt cgcgccggaa
     2761 cgcatcgccg cgcacttgat cgagatgttt acggactcgg agatcaaggg ctacgcgcat
     2821 cacggcaagt acgacatgca cgtgttgacg cgctacggca tcgatgtcca gaacctcgag
     2881 ttcgacacca tgatcgccgc gtaccttctc ggcgagaatt cggtcgggct caaggatctc
     2941 gccttctcac ggctcggcat cgagatgact gagatcacgg ccctcatcgg cactgggcga
     3001 aatcagctct cgatgagcgt cgtgccgctc gatcaagtcg ctcagtacgc atgtggtgac
     3061 gtcgaagcga cattcgagct ggtcaactac taccgcccgc gcctgaacga gaacaaccag
     3121 gaatggctgt tccgaaaggt cgagatgccg ctgatccagg tcctttccga gatggagcgg
     3181 atgggcatct cgatcgattg cgaatacctg gcggagttgg acaaggagat tcggcagcgg
     3241 ctgatggagc tggaccggga gatcacccgg ctcgccggtc gcccgatcaa cgtcaactcg
     3301 acccgacagc tcggtgcgct gctcttcgac gagttcaagc tgccgagcgg acggcggacg
     3361 aagaccggat actcggttga tcaagacctg ctcgagacgc tgcgcgacca gcacgcgatc
     3421 atcccggtca tcctcgagta caagacgttg ctcaagttgt cttcgaccta cgtctcggca
     3481 ctccctgccg aggtcgaccc gaagaccggc cgcattcaca cctcatataa ccagacggtg
     3541 gctgccaccg gccggctctc ctccaactcg cccaacttgc agaacatccc gatccggacc
     3601 gaggtcgggc ggcgcgtgcg acgcgcgttc gtggccgatc actccgagcg gcgcatcttc
     3661 gaagacagcg tcatgctctc ggccgactac tcgcagatcg agctacgctt gatggcccat
     3721 atgagcaacg agccgttcct gctcgaggcg tttcgtggcg gtgcggatat ccaccgggcg
     3781 acggccgcgc tggtctatgg catcgatccg gccgacgtca cgcccgacat gcgccgcgtc
     3841 gccaagacgg tcaacttcgg actgctctac ggcatgcagg cgtacggcct gtcgcgcgat
     3901 accgggctca gccgggctga cgcgcaagcg ttcatcgacc agtactgggc gcggcttccg
     3961 ggcgtcaaga cctactttga tgagacgctt cgccgcggcg aggaactcgg ttatgtcgag
     4021 acactttacg gccggcggcg agccgtgccg gacctgacgt cacccaacgg acagcgccgg
     4081 gcggccgcag tccgtgttgc gaccaatatg ccgctccagg gcacggcggc cgacatcatg
     4141 aagattgcca tggtccgcct ccaggagcag ctccgagacc ggtcggacag agctgtaatg
     4201 ctcctgcaag tccacgacga gctcgtgctc gaagtcaacc gaccggcgct cgatgagatc
     4261 ggcgccttga cgcgccaggt gatggaggat gcggccgaac ttgcggtgcc gctggtggcg
     4321 gatctctcgg ttggtccgaa ctgggatgac cagtccgatt tcgatccggc ctgatccgcg
     4381 agtggatatt gagcagacct gcctcactga ctagaattag tcgattacgg gttcgatctt
     4441 tgtacttgtt cgggacattg aatgcctaca tatcagtatc agtgccgcga ctgcggccat
     4501 gggttcgagc agttccagaa gttcagcgat gatccactca ctgtttgccc ctcctgcgag
     4561 ggcagtgtgc agcgagtgat tcagaacgtt ggcgtcgtgt tcaagggctc cggctggtac
     4621 ataaacgact cgcggaaacc cgagaaatcg gagagcggcg cgccggccga gg
//
LOCUS       DATAOZ010000151         6085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7428557, whole genome shotgun sequence.
ACCESSION   DATAOZ010000151 DATAOZ010000000
VERSION     DATAOZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7428557"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            490..1098
                     /locus_tag="VHR64_04130"
     CDS             490..1098
                     /locus_tag="VHR64_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX3302045.1"
                     /translation="MSGFVSRGFKGRRREPEAADRLPPGQYLTRDFPVLSAGPTPHTP
                     LDRWDFVVDGAIDQARRWTWDEFLALPREMPTVDIHCVTKWSKLDTVWEGVAIDTLLA
                     GAHHTARFLIAHCDGGYTTNLPLEDVTGGKAWVAFQYDGQPLDPEHGGPARLLVPHLY
                     FWKSAKWVRRLELVHDDRKGFWESLGYHNYGDPWREQRYWSD"
     gene            1086..1820
                     /locus_tag="VHR64_04135"
     CDS             1086..1820
                     /locus_tag="VHR64_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEX3302046.1"
                     /translation="MERLSWRLADVQEIVDETPRTKTLVLRVPEWAGHRPGQHVDVRL
                     TAEDGYQAERSYSIASAPDGERVELTIEMLEEGEVSTYLAGELRPGDQLELRGPIGGY
                     FTWEIADGGPLLLVGGGSGVVPLMAMLRQRAVLHSDVPARLLYSSRSWDEVIYREELT
                     LLDNADPALEVTHTLTRAQPLGWTGYQRRIDMAMLREVAWSPADRPLVYVCGPTRLVE
                     TVASTLVTLGHAPERIKTERFGPTGV"
     gene            1823..2095
                     /locus_tag="VHR64_04140"
     CDS             1823..2095
                     /locus_tag="VHR64_04140"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEX3302047.1"
                     /translation="MADGAVRLDGNAAAGLLEEIFTFDVTLAWVTCAGCGVEGPVAKL
                     AAYGMAMGAILRCPSCDTPVMRVSRLGTGYGLDLRGTTLMLVGQNS"
     gene            complement(2419..2907)
                     /locus_tag="VHR64_04145"
     CDS             complement(2419..2907)
                     /locus_tag="VHR64_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302048.1"
                     /translation="MATQQGSLDLLADPVAQQLLTSTLPARLAYVWTDGTPRVVPIGF
                     HWNGSELVIGTFPDAPKMSALRNGDKVAISIDGATFPYKVLLVRGSIRTDIVDGIAPE
                     YAAMCKRVFGEEQGEAWLDQVRPIAPQMARVVVTPEWVGILDFETRFPSALERAMERA
                     HA"
     gene            3069..3371
                     /locus_tag="VHR64_04150"
     CDS             3069..3371
                     /locus_tag="VHR64_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX3302049.1"
                     /translation="MVEEVLHRVRGNTSVAVTTITSKGQITLPAEFRKREGFHPGDKV
                     IVRECDGGLIVESATAAADRLYGMLADVAAGKPPATIEEWDESAAAGWADEPIEPK"
     gene            3368..3790
                     /locus_tag="VHR64_04155"
     CDS             3368..3790
                     /locus_tag="VHR64_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX3302050.1"
                     /translation="MIDAGILDSNVLLRYILRDIPDQADQTRTLLRKIGVGEAEAVLL
                     PTIFLEVVYVLERQYRLGRDRIGDTLYEILMLNGIRIVDREQLLDAAIEYRRRPGISF
                     ADAYHCAMARDFHSGSIISFDRKLNGIPGVTRRDPEEV"
     gene            complement(3849..4883)
                     /locus_tag="VHR64_04160"
     CDS             complement(3849..4883)
                     /locus_tag="VHR64_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX3302051.1"
                     /translation="MKPIVFDPPTCDPQSALGGVTPDDIRIGGKRRFRKGHRFTADDL
                     PDLARVEHPIHIIQIEADEIHEDDAAIALAEAIAGQGIERRNPVQSRVNLIAARKGLL
                     RVDREAVIALNRLTGISVFTLLDGLAVVPGKNIAGAKIAPVAIPKAMLDEALAIAARN
                     PVVEVKPFTPKRVSVVTTEGLGEKVWDRFRASVEQKIGWYGSSVTEFRDLLDDPDRVA
                     EAITGAIDDGADVILTGGGNALDPLDPTIRALNDIGAEFVKFGAPAHPGSMFWLAYKG
                     STPIFSLASCSLYSRSTVADVVLPWIMADEQVELDDMAGIGYGGLLDREMSFRFPPYD
                     AAEVHEPDEE"
     gene            5057..5752
                     /locus_tag="VHR64_04165"
     CDS             5057..5752
                     /locus_tag="VHR64_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3302052.1"
                     /translation="MHPVDEGAVGSIRFLRWPERGRRGQEFSEEFFALDQDPDAVLAA
                     VRRAEPGPDHLIAEVSVQSEPAREAYLAAGYEIGSQEPLMAAHLTDANTVPDPDGAVR
                     RVTSMDDVEQILAAQRSVGYPERLFSQALLDDPQVSIRPIFADGAFAAIGKLAVVTSG
                     AYITDVMTMPGHRRQGLGAAVMRQLHADARTIGAIRSVLTSTAMARSLYERLGYRQIG
                     LVTLYATPERIAG"
     gene            complement(5749..>6085)
                     /locus_tag="VHR64_04170"
     CDS             complement(5749..>6085)
                     /locus_tag="VHR64_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX3302053.1"
                     /translation="NLGTPAAAGEVQITLTEMSVAASSTTFKVGQTYTFTATNAGKIE
                     HELVFEKAGDNDVPLESNGAEVELEGIAPGASKSLDFAFTEAGTYQLACHVPGHYAAG
                     MVLTIYVVS"
BASE COUNT         1065 a         1774 c         2134 g         1112 t
ORIGIN      
        1 ctgggcggga ccggagagga agacggcgag cgaggccagc gccaacatca aaccgccccg
       61 aaacggaacc tcactcggtc ggcggcgaat catcaggcgc ggtgctctca tcggtgcgcg
      121 ataggactgg tcgttgcgtg atcttaaccg aggcgaggag gcgagaaggc gagcagttgg
      181 agcgcggagt cgcgaaggcg cggagacggg gaggtgcgga gatgaatgta gggatttgcc
      241 gtggtaaatc cgcacgttgc cgaaacaaac gcggcccggt gtgcgatatg ggatcggggc
      301 cgaacccggg attggcggcc attgcaggac ccggggatcc aggggcgggt ttgccgcggc
      361 aaacccctac agagattgtg gtgatccacc cgcctgaccg gtatgctctc cccgaacccc
      421 gaacccccac ttttcgccgg gcatacaatg ggtttctgac cttcgtggtt gcaccggagg
      481 caatgcatca tgagcggatt cgtttcgcgt ggattcaagg gtcggcgacg ggagccggag
      541 gccgcggatc gcctcccgcc cgggcaatat ctgacccggg actttcccgt cctctccgcc
      601 gggccgaccc cgcacactcc cctcgaccga tgggacttcg tagtcgacgg ggcgatcgat
      661 caggcgcggc ggtggacatg ggacgagttc ctggcgctgc ctcgggagat gccgacagtc
      721 gatatccact gcgtgaccaa gtggtcgaag ctggatacgg tctgggaggg ggtcgcgatc
      781 gacactctgc tggccggcgc gcatcatacc gcgcgcttcc tgatcgccca ttgcgatggc
      841 gggtacacga ctaatttgcc gctcgaggac gtaaccggag gtaaggcgtg ggtggcgttc
      901 cagtacgatg gccagccgct cgatcccgag catggcggtc cggcccggtt gctggtgccg
      961 catttgtact tctggaagag cgccaaatgg gttcgtcgcc tggagctcgt ccacgacgat
     1021 cgcaagggct tttgggaatc gctcgggtac cacaattatg gtgacccatg gcgggaacag
     1081 cggtattgga gcgactgagc tggcggctcg ctgacgtcca ggagatcgtc gacgagacac
     1141 cgcggacgaa gaccctggtg cttcgtgtcc ccgagtgggc gggacaccgg cccgggcagc
     1201 atgtcgatgt ccggctgacc gcagaggatg gctaccaggc cgagcgcagt tactcgattg
     1261 cctccgctcc tgacggcgag cgggtcgagc tgacgatcga gatgctggag gagggcgaag
     1321 tctcgaccta cctggccggg gagctgcggc ccggcgatca actggagttg cgcggtccga
     1381 tcggcgggta tttcacctgg gagatcgcgg acggcgggcc gctgttgctg gtcggcggcg
     1441 gatcgggcgt ggtcccgctg atggcgatgc tccggcagcg ggcggtgttg catagcgacg
     1501 tcccggcgcg gctcctctac tcctcgcggt cgtgggacga ggtgatctac cgggaggagc
     1561 tgacgctgct cgacaacgcg gaccccgcgc tggaggtaac ccacacgctt acgcgcgcgc
     1621 agccgctggg ctggaccggg tatcagcggc ggatcgacat ggcgatgctg cgcgaggtcg
     1681 cctggtcgcc ggcggatcgc ccgctggtct atgtctgcgg tccgacccgg ctggtggaga
     1741 cggtggcatc gacgctggtg acgcttgggc atgcgccgga gcggatcaag acggagcgct
     1801 tcggcccgac aggagtttga ccatggcgga tggcgcggtc cggctcgacg gcaatgccgc
     1861 ggccggtttg ctcgaggaga tcttcacctt cgacgtcacg ctggcgtggg tgacctgcgc
     1921 cgggtgcggg gtggagggac cggtggcgaa gctggccgcc tacgggatgg cgatgggcgc
     1981 gatcttgcgc tgcccgagct gcgacacgcc ggtgatgcgg gtatcgaggc taggtactgg
     2041 gtacgggctc gacctgcgcg gcacgaccct gatgctggtt ggccagaatt cgtagcgtcg
     2101 catggcaatg cgacctcgat ctcgggatgt ccggtcgggt gcagggaacg atgttccccc
     2161 gcgccgcccg gggataaatc ccagggctac atccaataag cccgctaaag cagactggag
     2221 acgtagcgcc ggctcgcctc ttggtcgccc gagggtggga tgtgccgtcg ggttgggccg
     2281 gttcgccccc atctatcccc cgtgtcggtg cgcccatgcc ggccacgggg ggccggcgct
     2341 acgtcacggt acggtgccag tgtgagtcgg gggtcgcatt gcgatgcgac gctaccactt
     2401 ctccctgatc cctgatccct atgcgtgggc gcgctccatg gcgcgttcga gggcacttgg
     2461 gaagcgggtt tcgaagtcga ggatgccgac ccattccggc gtgacgacga cgcgcgccat
     2521 ttgcggggcg atcgggcgga cctgatcgag ccaagcctcg ccctgttcct cgccgaagac
     2581 gcgcttgcac atggccgcgt actccggcgc aatcccgtcg acgatgtcgg tccggatcga
     2641 cccccgcacg agcagcacct tgtacgggaa tgtggcgcca tcgatcgaga tcgccacctt
     2701 gtctccatta cgcaatgcgg acatcttcgg cgcatccggg aaggtgccga tcacgagctc
     2761 actcccattc cagtggaagc cgatcgggac gacgcggggt gtgccgtcgg tccagacata
     2821 ggcgaggcgg gcggggagag tggaggtcag gagctgttgg gcgacgggat cggcaaggag
     2881 atcgagactg ccttgttgcg ttgccatgaa gttcctccgg gatagtggtt agttgcacaa
     2941 ggggcggcac ccagttgttt gcgatgcatt acttctacgc ccgctggagg tgcaatgcaa
     3001 gcaggtgggg cttggtgaga ggcgatcggc gaactgcggt gcgcgccggc ttgaccgagt
     3061 gtaatacgat ggtcgaagaa gtattacatc gagtgagagg taatacgagc gtggcagtga
     3121 cgaccattac gtcaaaggga cagatcacgt tgccagcgga gtttcgaaaa cgcgaggggt
     3181 tccatcccgg cgataaagta attgtgcgcg agtgcgacgg tggattgatt gtcgaatcgg
     3241 cgacagctgc ggctgatcgg ctctatggga tgctggcgga tgtcgcggcg ggcaagccac
     3301 cggccacaat tgaagagtgg gacgagtctg ccgcggccgg ttgggctgac gaaccgatcg
     3361 agccaaaatg atcgacgccg gaatcctcga ctcgaatgtt ctccttcgat acatcctgcg
     3421 ggacataccg gatcaagccg atcaaaccag gacgttgctg cggaaaatcg gcgtcggaga
     3481 ggcggaagcg gtactcctcc ccactatctt cctcgaggtc gtctacgtct tggaacggca
     3541 atatcgactg ggacgagacc gtatcggcga cacgttgtac gagatcctga tgttgaacgg
     3601 aatccgcatt gttgacaggg agcaattgct cgacgcggct attgaatacc ggcggcgacc
     3661 tggtatctca tttgcagacg cgtatcactg tgcgatggcg cgggatttcc attccggcag
     3721 catcatctcg ttcgaccgca aattgaacgg catcccaggc gtcacccgga gggaccccga
     3781 agaggtctga aaaggtttct tgtctttcgt ctttggtttc ccctttttcc gttccctcta
     3841 cccgcgattc actcctcgtc cggctcgtgg acttcggcgg cgtcgtaggg tgggaagcgg
     3901 aaggacatct cgcggtcgag gagaccgccg tagccgatcc cagccatgtc gtccagctcg
     3961 acctgctcgt cggccatgat ccagggcagg acgacgtcag ctacggtcga gcgtgagtag
     4021 agcgagcagg aggcgaggct gaagatcggc gtgcttcctt tatatgccag ccagaacatg
     4081 ctgccggggt gggccggagc gccgaacttg acgaactccg cgccgatgtc gttcagggcg
     4141 cgaatcgtcg gatcgagtgg gtcgagcgcg ttgccgccgc cggtcaggat gacgtccgcg
     4201 ccatcgtcga tcgccccggt gatcgcctcg gccacgcggt ccgggtcatc gagcaggtca
     4261 cggaactcgg tcacgctgct gccgtaccag ccgatcttct gctcgacaga tgcccggaag
     4321 cgatcccaga ccttctcgcc gagtccttcg gtggtgacga ccgagacgcg cttgggcgtg
     4381 aacggcttga cctcgacgac cggattccgg gcagcgatgg ccaacgcctc gtccagcatg
     4441 gctttcggga tggcgaccgg agcgatcttg gcgccggcga tgttcttgcc gggcacgacc
     4501 gcgaggccat cgagcagcgt gaagaccgag ataccggtta gccggttgag cgctatcacg
     4561 gcctcccgat cgacccggag caaacccttg cgggcggcga tgaggttgac ccggctctgg
     4621 accgggttgc gtcgctcgat gccttggccg gcgattgcct cggccagcgc gatcgcggcg
     4681 tcgtcctcgt gaatctcgtc cgcttcgatc tggatgatgt ggatcggatg ctcgaccctg
     4741 gccagatcgg gcagatcgtc ggcggtgaag cgatgtccct tgcggaagcg gcgctttccg
     4801 ccgatccgga tgtcgtccgg ggtgacgccg ccgagcgcgg actgcggatc gcaggtcggg
     4861 ggatcgaaga cgatgggttt catgtgagta gtgtaggcga gcaggcgtgg ttaagggtga
     4921 aggctggagg gttgagggaa cgaaaaacgg aaaacggaaa accaaagacg aaagaccaga
     4981 gaccaaagac gaacgaccag agaccaaaga cctcttcgaa ctgctcattc gcgggtttgc
     5041 gcggttggag cgcggggtgc atccggttga tgagggcgcg gttgggtcga tccggtttct
     5101 gcgttggccg gagcgaggac ggcggggaca ggagttctcc gaggagttct tcgcgctcga
     5161 tcaggatcct gacgcggtgc tggcggccgt gcgcagggca gagccgggtc ccgatcacct
     5221 gattgccgag gtttcagtgc aatccgagcc ggcgcgggag gcgtaccttg ccgccgggta
     5281 tgaaattggg tcgcaggagc cattgatggc ggctcatctg accgatgcca ataccgtgcc
     5341 cgatcccgac ggggctgtcc gccgggtaac ctcgatggac gatgtcgagc agattctggc
     5401 ggcgcagcgg tcggtcggat acccggagcg actgttcagc caggcgctgc tcgatgaccc
     5461 gcaggtcagc atccggccta tctttgcgga tggagcattc gcggcaatcg gcaagttggc
     5521 agtcgtcacg tccggcgcct acatcacgga cgtgatgacg atgcccgggc accggcggca
     5581 ggggcttggc gccgcagtca tgcgccaatt gcacgccgac gcccggacga taggcgcgat
     5641 ccgctcagtc ctgacctcga ccgcgatggc gagatcgctc tacgagcgac tcggctaccg
     5701 gcagatcggg ctcgtcacgc tctacgcgac gcctgagcgg attgccggct agctgacgac
     5761 gtagatcgtc agcaccatgc cggctgcgta atggcccggc acgtggcagg cgagttgata
     5821 ggtgccggcc tcggtgaacg cgaagtcgag cgacttggat gcgcccggcg cgatcccttc
     5881 gagctcgacc tcggcgccgt tactttcgag cggcacgtca ttgtcaccgg ccttctcgaa
     5941 aaccagctcg tgctcgatct tgcccgcgtt tgtggcggtg aaggtgtagg tctggccgac
     6001 tttgaacgtg gtggacgatg ccgcaacgga catctcggtg agcgtaatct ggacctctcc
     6061 agcagcggcc ggagtgccga ggttg
//
LOCUS       DATAOZ010000152         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3218424, whole genome shotgun sequence.
ACCESSION   DATAOZ010000152 DATAOZ010000000
VERSION     DATAOZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3218424"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VHR64_04175"
     CDS             <1..464
                     /locus_tag="VHR64_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEX3302054.1"
                     /translation="FGYNRAEDERQILAGRQLACHFTDVVSRGGRLLLNVGPTAEGEI
                     PELQQASLRSLGRWMTQVGDVIRAAQPVSRSVARPTNEPWIRWLETPDHLVALVDQTG
                     EVTLDVDGNAVTAGEAKVLAAPGAARVTGGAMRVSVDELTDGPAAILISKH"
     gene            495..1499
                     /locus_tag="VHR64_04180"
     CDS             495..1499
                     /locus_tag="VHR64_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3302055.1"
                     /translation="MKYGELGRSGMNVSKICLGTMHFGPRATEEESHAILDKALSMGI
                     TFIDTANVYGGETARGRSEEIIGTWFAARPGVRDEVVLATKVYQAMGDLGPANNDRGF
                     SAYKVHKHLADSLRRLQTDRIDVYQVHHIDERVSAEEFWGTYERIVADGDVLYAGSSN
                     FSGWGLAKRQMQAWQRGFVGFVSEQTQYNLLSRVPEMEVLPAAQDFGIGVIVYMPLAG
                     GLLTGKTESFDGSRTRQVEEEYGIRIGAENHQFRDFSDFCRELGEPEQVVATAWVLQH
                     RAVASAIVGVRTVDQLAGLDRAAELELDEAASQRLNEIFDINNGRRIGQGRSPEAHSW
                     "
     gene            complement(1514..1666)
                     /locus_tag="VHR64_04185"
     CDS             complement(1514..1666)
                     /locus_tag="VHR64_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302056.1"
                     /translation="MVDMICFTLDSAAALVEALRERSGDLIHCGTIWRYGPSHDQLEV
                     ANRLVI"
BASE COUNT          324 a          548 c          587 g          322 t
ORIGIN      
        1 ccttcggcta caaccgcgcg gaggacgagc gacagatcct ggccggccgc cagttggcat
       61 gccatttcac tgacgtcgtg tcccgcggcg gacggttgct gctgaacgtc gggccgaccg
      121 ccgaaggaga aattcccgag ctccagcagg cgtccctgcg gtcacttggc cgctggatga
      181 cccaggtcgg cgacgtcatt cgggcggcgc agccggtgtc gcgcagtgtt gcccggccga
      241 cgaacgagcc atggatacgc tggctcgaaa cgccggacca tctggtggcg ctggtcgatc
      301 agacaggcga ggtgacactc gatgtcgatg ggaacgccgt cacggcgggt gaggccaagg
      361 tactagctgc gccgggtgcg gcgcgcgtca ccggtggcgc catgcgggtg agcgtcgatg
      421 agctcaccga cggcccggcc gccatcctga tttccaagca ctgatcgaga atcgaattcg
      481 aaaggcatga tcacatgaag tacggcgaac tgggtcgttc tggcatgaat gtcagcaaga
      541 tctgtctcgg caccatgcac ttcggcccga gggcgaccga ggaggagtcg cacgcgatct
      601 tggacaaggc gctctccatg ggcatcacct tcatcgacac cgcgaacgtc tacggcggtg
      661 aaaccgctcg gggccgctcg gaggagatta tcggcacctg gtttgccgcc cggccgggcg
      721 tgcgtgacga ggtcgtgttg gcgacaaagg tctaccaggc aatgggcgat cttggtccgg
      781 ccaacaacga ccgcggcttc tcggcgtaca aagttcacaa gcatctggcg gactccttgc
      841 gtcggctcca gacggaccgg atcgacgtct accaggtgca ccacatcgac gaacgggtgt
      901 ccgcggagga gttctgggga acgtacgagc gcatcgtggc cgacggagac gtgctctacg
      961 cgggttcgag caacttctcc ggctggggtt tggccaagcg gcagatgcag gcttggcagc
     1021 gtggcttcgt cggcttcgtg tccgagcaga cgcagtacaa cttgttgagc cgggtgcctg
     1081 agatggaggt tctcccggcc gcgcaggact ttgggatcgg agtgatcgtc tacatgccat
     1141 tggcgggcgg cctgctgacc ggcaagaccg aatcctttga cggcagccga acccgtcaag
     1201 ttgaggagga gtacggaatc cggatcggtg ccgagaacca ccagtttcgc gacttctcag
     1261 atttttgtcg tgagctcggg gaaccggagc aggtcgtcgc gaccgcttgg gtgctccagc
     1321 accgcgccgt cgcttcggcc atcgttggcg ttcggaccgt tgatcagctg gccgggctcg
     1381 atcgggctgc cgaactcgag ctcgatgagg cagcatcgca acggctgaac gagatcttcg
     1441 acatcaacaa cgggcgcaga atcggccaag gtcgttctcc cgaggcccac tcatggtgat
     1501 cgcttcggac ttgttagatg actagccggt tggcgacttc gagttgatca tgactcggtc
     1561 cgtatcgcca gatggtcccg cagtggatca ggtccccgct gcgttcgcgg agcgcctcca
     1621 ccagggcagc cgccgagtcg agcgtgaagc agatcatgtc gaccacgacg tcggcctcga
     1681 gggcagcgac cgctttccga acgttccacc cgcctcctcg gcctcccgat ccaccgtgac
     1741 ctgctcgacc tcggaccacg cctcgtcctc gacataagca a
//
LOCUS       DATAOZ010000153         4975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7954846, whole genome shotgun sequence.
ACCESSION   DATAOZ010000153 DATAOZ010000000
VERSION     DATAOZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7954846"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1376)
                     /locus_tag="VHR64_04190"
     CDS             complement(96..1376)
                     /locus_tag="VHR64_04190"
                     /inference="COORDINATES: protein motif:HMM:NF005373.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX3302057.1"
                     /translation="MDQTLAQSIGETIDRLESELVERLQELIRIPSVTGSEHDVQQAV
                     ARQMNDVSLDIDMWEPDPAELAPYAEHVGAFESLAGRPNVVGTRRGSGGGKSMILNAH
                     IDTVEPGDPAQWTVDPFLAEIKDGRLYGRGACDMKAGLVAELMALRALDELGITLNGA
                     VFVESVISEEDGGAGALATILRGYTADGAIITEPTNLAIISAQGGSLVFRLTITGRSA
                     HACVRNEGVSALEKFLPIHAAILEHERQHHEAIDHPLYERFANRAPINIGTVRSGNWP
                     SSVPESIVVEGRAGLVPGETLEKTKTALVEVIDQVAASDPWLSEHPPLIEWFSGQFTA
                     AETPLDSPLMHTLQNAHLQTTGARPIIDAATYGADMRHFVNIAGIPCLMYGAGDVARA
                     HRPDEFVPISEVKTVARTLALTLVDWCGVHNSPA"
     gene            complement(1411..2586)
                     /locus_tag="VHR64_04195"
     CDS             complement(1411..2586)
                     /locus_tag="VHR64_04195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3302058.1"
                     /translation="MTSSNGIVIIGAGVIGASIAAHLAMRGERPLVLERAHVCSGTTG
                     QSGGLVRQHYSNPKTAALARDALAIFRRWSDHFEGSSGFVPVGVLLTAGPETEASVRA
                     NVAMHKELGIETTLLAPDDIAAIDPRVHAGDCTTICYEPTAGIADPIETNHAFAATAR
                     SLGVEIREDVSVDEIVVESGRVAGLRTSHGEIAADIVVDAAGAWGIQFLTTLGHELPI
                     TFSRHPMALIRRPPAEIDRHPAVLDVHTDSYFLPRADATLVGKLGTMPDDLDVDPDGY
                     ERGVTNAEIERYRAAARVRMPFLDRGTVLGGWAGIYDDSIDAHPIVDAVPGSEGLFCA
                     LGMSGNCFKLSPVIGDLLAQRIIEGPGSTPKLDLFRFDRFRDGSAHDRAFSAMSVLA"
     gene            complement(2583..3500)
                     /locus_tag="VHR64_04200"
     CDS             complement(2583..3500)
                     /locus_tag="VHR64_04200"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302059.1"
                     /translation="MTADGRVVAAIDIGSNTLKLTVARVSDGKVTPIASTAEVVRLST
                     DLGQTGRLREDRMQHAIDVLRDFVTIAREYEAGQIIAVGTAATRSAANGTEFLDRVKN
                     ETGIDVDVISGGEEARLTSNGVLAQIDPAGMILIVDIGGASTELIAVRDGIRLESASL
                     GIGSGVLTDRFVGSDPPRDSELDHVERETARLASAFLGHYEAVDRLVLVGGVGDFLFG
                     LLQKTGYIDATALDTAREIVLGMTAADLAPVINAPVARARVLPTGFAIARAICRESGA
                     PLIESVANGLRIGILLRVAASGAQEESPR"
     gene            3664..4581
                     /gene="rsgA"
                     /locus_tag="VHR64_04205"
     CDS             3664..4581
                     /gene="rsgA"
                     /locus_tag="VHR64_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX3302060.1"
                     /translation="MGKQQQLTGTVVKAYGKFFEVALDDSDESLLSTPKGLLKRERKG
                     TDIVAVGDRVTVIDVGDGEGRIEFVEPRRSVLARLARHTRDTEQVLVANPDQALFLFA
                     ATEPVPHPRMLDRFLVLAESRDLPALIGVNKIDLLAGDPAQIFPEHRAIYPVIDISVQ
                     TGQGLHHLRELLEGRLTVVAGPSGVGKSSLVNTLNPDLGQAVSSVSESTGKGKHTTTA
                     AQLFRLGTDTYIADTPGIRALALQGVAPEDLPECFPEFRPFLGHCRFNDCTHVHEPGC
                     AIREAVGSGEVGFDRYESYVSLRRGDPEV"
     gene            complement(4578..>4975)
                     /locus_tag="VHR64_04210"
     CDS             complement(4578..>4975)
                     /locus_tag="VHR64_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302061.1"
                     /translation="QRAHIASALMDADRYDRHLWNVPGLRWQQVDGDHEIEPGLALVR
                     TDGHAIGHQSVLVETRGGLVILAADALDSARMADRRQFPDYYDVDATNRSIDRLLALG
                     QERGAPIIYGHDWEQWSTLPHSPEPYSPS"
BASE COUNT          858 a         1594 c         1615 g          908 t
ORIGIN      
        1 taggccgaag ttgactcgat gcaaaggccg tgtccatgga tgcacaggat ttgccactca
       61 atctgccgca gtgtttgcac aggattttcg tgcgattacg ccgggctgtt gtgcacacca
      121 caccaatcga ctaaggtcag ggccaaggtc ctcgcaacgg ttttgacctc tgaaatcggc
      181 acgaattcgt ccggccggtg cgccctcgcg acgtctccag cgccgtacat caggcatgga
      241 atgccggcaa tgttgacgaa gtggcgcatg tcggccccat aggtggcggc gtcaatgatg
      301 ggacgcgctc cggtcgtctg taggtgtgcg ttctgtagcg tgtgcatcaa ggggctgtcc
      361 aggggggttt cggccgccgt gaactgccca ctgaaccatt cgatcagtgg cggatgttcc
      421 gacaaccaag ggtcgctggc tgccacctga tcaatgactt caaccagcgc tgtcttcgtc
      481 ttctcgagcg tctcgcccgg tacaagaccc gcccgtcctt cgaccacgat cgactcgggt
      541 actgagctcg gccagtttcc ggaccggacc gttccgatat tgatcggcgc gcgattggcg
      601 aaccgctcat agagcggatg atcgatcgcc tcgtgatgtt gccgctcatg ctccaggatc
      661 gcggcatgga tcggcaggaa cttttcgagc gcgctcacgc cttcgttgcg cacgcaggca
      721 tgggccgagc ggccggtgat cgtgagtcgg aacaccagag agccgccctg ggccgagatg
      781 atggcgaggt ttgtcggctc ggtaatgatc gcgccgtcgg cggtgtatcc gcgcaggatc
      841 gtcgcgagcg caccggcgcc gccatcctcc tccgagatca cgctctcgac gaagacggcg
      901 ccgttcagcg tgatgccgag ctcatccagc gcacgcaggg ccatcagctc agcgacgagc
      961 ccagccttca tgtcgcatgc cccgcgccca tagagccggc cgtccttgat ctcggcgagg
     1021 aatggatcga ccgtccattg ggccggatcg cccggctcaa ccgtgtcgat gtgcgcgttc
     1081 aggatcatcg acttgccgcc gcctgaaccg cgccgggttc caaccacatt cgggcgacca
     1141 gcgagcgact cgaacgcgcc gacgtgctcg gcatatggcg cgagctcggc cgggtccggt
     1201 tcccacatgt cgatatcgag cgagacatcg ttcatttggc gcgcgaccgc ctgctgaacg
     1261 tcgtgctccg agccggtcac gctcggaatg cgaatcagct cctgcaaccg ctcgacaagt
     1321 tccgattcaa ggcgatcgat cgtctccccg atcgactggg caagcgtctg atccacatct
     1381 gattcctttc tcaatgatct cgtgacggtg tcaggccaaa acgctcatgg cactgaaagc
     1441 gcgatcgtgc gccgagccgt cccggaaccg atcgaaccgg aagagatcga gcttcggcgt
     1501 cgagccggga ccctcgatga tccgctgggc gagcagatcg ccgatcaccg gcgagagctt
     1561 gaagcaattg ccgctcattc cgagcgcgca gaagagccct tccgatccgg gtacggcgtc
     1621 gacgatcggg tgggcgtcga tcgaatcgtc gtagataccg gcccagccgc cgaggaccgt
     1681 gccccggtcg aggaacggca tgcgtacgcg ggcggcggcg cgatagcgct cgatctcggc
     1741 gtttgtcacc ccgcgctcgt agccgtcggg atccacgtcc aggtcgtccg gcatcgtgcc
     1801 cagcttgcca acaagcgtcg catcggcgcg tggcaggaaa taggagtcgg tgtgcacatc
     1861 gagcacagcc gggtgtcgat cgatctcggc cggcgggcgc cgaatcaggg ccatcggatg
     1921 gcgcgagaag gtgatcggca actcatgacc caacgtggtc agaaactgaa ttccccatgc
     1981 gccggcggca tcgaccacga tgtctgccgc gatctcgcca tgactcgtgc gcagcccggc
     2041 gacgcgacct gactcgacga cgatctcatc gaccgacacg tcctcgcgga tctcgacgcc
     2101 gagcgagcga gcggtagcgg cgaaggcatg attcgtctcg atcggatcgg cgatgccggc
     2161 ggtcggctcg tagcagatgg tcgtgcagtc gccggcgtgg acgcgcgggt cgatcgccgc
     2221 gatatcgtcg ggggcaagga gtgtcgtctc gatgccgagc tccttgtgca tggcgacgtt
     2281 ggcccggacg ctggcttcgg tctcgggtcc ggcggtcagg agcaccccga ccggtacgaa
     2341 gccagagctt ccctcgaagt ggtccgacca gcgccgaaag atcgcgagcg catcgcgggc
     2401 gagggcagct gtcttcgggt tcgagtagtg ttgccggacc aggccgccgc tttgaccggt
     2461 cgtcccggag cagacgtgtg cgcgctcgag aacgagaggt cgctcgccgc gcatggcaag
     2521 atgggcggcg atgctggcgc cgatgacgcc cgctccgatg atcacgatgc cgttcgacga
     2581 agtcatcgcg gtgattcctc ctgggctccg gacgctgcaa cacgcaacag gattccgatc
     2641 cgcagtccgt ttgctactga ttcaatgagc ggcgctcccg actcccggca gatcgcgcgg
     2701 gcaatggcga acccggtcgg tagcacgcgt gcacgcgcaa ccggcgcgtt tatcaccggc
     2761 gccaggtcgg ccgctgtcat gccgagcacg atctcccgtg ccgtgtcgag ggccgtggcg
     2821 tcgatgtacc ccgttttctg gagcaacccg aacaggaaat cgcccacacc gcccaccagc
     2881 accagccgat cgacggcttc gtaatggccg aggaacgccg acgcgagacg cgctgtttcc
     2941 cgctcgacgt gatcgagctc agaatcgcgc ggcgggtcgc tcccgacaaa tcggtcggtc
     3001 aggacgccgg agccgatgcc gagcgaggcc gattcaagcc gaatcccatc tcgaacggcg
     3061 atcagctctg tgctcgcacc gccgatatcg acgatcagga tcatcccggc cgggtcgatc
     3121 tgggccagga cgccgttgct tgtcagccgg gcttcctcgc cgccgctgat cacgtcgacg
     3181 tcgattccgg tctcgttctt gacacgatcg aggaattccg ttccgttcgc ggccgaccgg
     3241 gttgctgccg tgcccacggc gatgatctga ccggcttcgt actcacgggc gatcgttacg
     3301 aaatcacgca gcacgtcgat cgcgtgctgc attcgatcct cgcggagccg gccggtttgc
     3361 ccgaggtcgg tactgagccg aacgacttcg gcggtactgg cgatcggcgt gacctttccg
     3421 tcggagacgc gcgccacagt gagcttgagg gtattcgagc cgatatcgat cgcggccacg
     3481 acgcgcccat cggcagtcat gatctggagt ccgttcgacg cataccgatg ggccggcggg
     3541 actgcactgc tgtcgaaatg ctgctcatct cacccgatcc ggttccggct gcgtacctgc
     3601 tcgttgcatt cgtgatgata attgagcagc atctggacac gggcgacgtc tttgggggga
     3661 tggttgggga agcagcagca gctgaccggc actgtggtca aagcctatgg caagtttttc
     3721 gaggttgcgc tcgacgactc ggacgagtcg ctcctctcca cgcccaaggg actcctgaag
     3781 cgagagcgaa aggggacgga catcgtcgcg gtcggggatc gcgtgacggt gatcgatgtc
     3841 ggcgatgggg aagggcggat cgagttcgtc gagccgagac ggtcggtgct ggcccggctc
     3901 gcccggcaca cgcgcgacac cgagcaggtg ctggtcgcca accccgatca ggcgctcttc
     3961 ctgtttgcgg caaccgagcc agtgccgcat ccacgcatgc tcgatcgatt tctcgtgctc
     4021 gccgaatcgc gggacctgcc cgcgctcatc ggggtcaaca agatcgactt gctggctggt
     4081 gatcccgcgc agatctttcc cgagcaccgc gcgatctacc ccgtgatcga catcagcgtt
     4141 cagaccggcc aggggctgca ccatttgcgc gagctgctgg agggcaggct tacggtcgtg
     4201 gccgggccat ccggtgtcgg gaagtccagc ctggtcaaca cgctcaaccc cgacctgggc
     4261 caggccgtgt cgtccgtttc cgagagcacc ggcaagggca agcacacgac gaccgccgcc
     4321 cagctctttc ggctgggcac agacacctac atcgccgaca cgccgggaat tcgggcgcta
     4381 gctctgcaag gcgtcgcgcc ggaggatttg ccggagtgct tcccggagtt ccggccattc
     4441 ctgggccatt gccggttcaa cgattgcacg catgttcacg aacccggctg cgcgattcgt
     4501 gaagcggtcg gatctggcga ggtcggcttc gaccggtacg agagttacgt ctcccttcgc
     4561 cgcggcgacc cggaggtcta ggacggcgaa tatggctcgg ggctatgcgg cagggtcgac
     4621 cactgctccc aatcatgtcc gtagatgatc ggcgccccgc gctcctggcc cagggcaagt
     4681 aaccggtcga tcgagcgatt cgtggcgtcc acatcgtagt agtcgggaaa ctggcgccgg
     4741 tcggccattc gcgcgctgtc aagtgcgtca gctgccagga tgaccagacc gccccttgtc
     4801 tcgacgagca ctgattggtg gccgatggcg tggccatccg tccgtacgag cgccagtccg
     4861 ggctcgatct cgtggtcgcc gtcgacctgc tgccagcgca gtccgggcac gttccagagg
     4921 tgacggtcgt atcggtcagc atccatgagt gcggacgcga tgtgcgcgcg ctgga
//
LOCUS       DATAOZ010000154         5916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6639868, whole genome shotgun sequence.
ACCESSION   DATAOZ010000154 DATAOZ010000000
VERSION     DATAOZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6639868"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VHR64_04215"
     CDS             complement(<1..815)
                     /locus_tag="VHR64_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3302062.1"
                     /translation="MNAQKAVRPLIVAVTIALVALGAGWSSGLFTAQAQDATGFTAGQ
                     TVVVNTDGLTLRSDVGTASDALEVLPQGAFAEILDGPIADGDYTWYLVSFDNLSGYVA
                     GEFLADAASTGSFAVGDTVTVVTDALNLRDAATTAGNVTDVLGTGAVGTVLAGPVDAD
                     GYAWYQIDVDGVTSWAARPFLAYGALSAATTDTTTTTGSGSLLVNTDVLNVRDAASTS
                     ASVLTTLAEGDSVTATGNVITADGYDWTEITTADGTTAWVVADYLTSDSNALLL"
     gene            complement(981..1196)
                     /locus_tag="VHR64_04220"
     CDS             complement(981..1196)
                     /locus_tag="VHR64_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302063.1"
                     /translation="MSRIKRHPFASVIAAVALIAMISVIAFAAYLASATGSLPWQEDP
                     TRIPVTPFTGIPGFTPPTPLPTATKAP"
     gene            complement(1266..2033)
                     /locus_tag="VHR64_04225"
     CDS             complement(1266..2033)
                     /locus_tag="VHR64_04225"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX3302064.1"
                     /translation="MRTRSALLHRMVRWTAGPALLLALMLGLAANIAIAQDQSTPEST
                     PEAGVLDQNPVYIACDENVSAADGSDATPIPATSPATTWTLTADSAARYKAQEELANK
                     GANEAVGQTNSIVGQIYFDDQGMPLACSRWDVDLRTLTSDESRRDNYLRGNTLQTDTY
                     PVATFVLTSVEGLDQPLQDGQETSFSLVGNLTMHGVTKLVRWNITAKLDGDTLTGSGS
                     TSFNMEDYNIEEPRVGPVLSVDHHVTLEVDIVATKSA"
     gene            2286..3182
                     /locus_tag="VHR64_04230"
     CDS             2286..3182
                     /locus_tag="VHR64_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX3302065.1"
                     /translation="MNRTTAPGNDHDEGTDVQSRRVVLKAGATALGMSTLGIGALGAA
                     AQSDSTTPTNPALDATPTSCVLTPELTQGPYYLADELIRADITEGRAGVPLTLRIGVS
                     DINACGPLANAAVHVWHCDAHGFYSGVSANSPGSDATQQQIDEAAKATFLRGIQMTDE
                     DGFVEFKTIYPGWYRGRTIHIHMMVAVDGTAEQTYEGGHIAHTGQLFFDDALSDKIFA
                     TEAYTGRPEDERTLNEEDGILGDHDEEPGFMLALTPNNEERIEDGFVGVIAVGVDPTS
                     TPAAAGGPGGGQPGGPPPNGNR"
     gene            complement(3576..4910)
                     /locus_tag="VHR64_04235"
     CDS             complement(3576..4910)
                     /locus_tag="VHR64_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302066.1"
                     /translation="MPASADRPARRPSNSWAERFGLPHLDRPGAVTLYVATWIDAVGS
                     GLFLTFYLLYLTKAAGFSLGTAGAVLSLASGLALASNPIAGSLVDKIGARRMMLASQI
                     ICAVGYFSLLFVKGSVPLLIVASTLAVLGERIFWVGFPSFISIMAPDNERDRWFAFMG
                     MTREAGFGVGGLLAALIVALAGTDGYRLLVLLNACTFAIAAIIIYLRVPSPEVEPIHH
                     DHGGWRAVVRDRPVMKMAVANTVAVMTVLIGGLAMPIYVVDDLDLPAWLPGLLFVATT
                     IVLAFGQSLGLRVVTGWLRTRVYLLAAAIWVVGAIVFAFAQIVPRSLLIPYMFFAIVV
                     TIGGDLFHAPQTNGLPSALAPAALRGRYLALFSLSWGIGRTIAPSIVSGLLWLGPTWP
                     WVGMVLMACFAAAIALHTERELDPDRQRMPRTSRLDDVIEDDLLEPVEQVAA"
     gene            5112..5378
                     /locus_tag="VHR64_04240"
     CDS             5112..5378
                     /locus_tag="VHR64_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302067.1"
                     /translation="MSKKQVALEVTMMLAAQQLVDRWLRSPGRGPFQRAVTQEVQARW
                     SDMPGTEAQRRAFRRLANAIRVGEGHAIANPGPLLKIARQNLRP"
     gene            5513..>5916
                     /locus_tag="VHR64_04245"
     CDS             5513..>5916
                     /locus_tag="VHR64_04245"
                     /inference="COORDINATES: protein motif:HMM:NF017709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I9 family protein"
                     /protein_id="HEX3302068.1"
                     /translation="MPRTVLIRLIGAVLLLAVQAADFACVPVQGVHAQSGTTIQVAPG
                     RYIVLLKPATGVTSAGVAAGYDARPGVTVDHVYTSAISGFAGTIDRAAAQALVNDPRV
                     AGVFPDIISHAASQSLPAGIDRVDADLNPTKAG"
BASE COUNT         1053 a         1753 c         2130 g          980 t
ORIGIN      
        1 aggagcaagg cattgctatc gctcgtcagg taatcggcga cgacccaggc ggtcgtgccg
       61 tcggccgtcg tgatctcggt ccaatcgtag ccgtcggccg tgatgacatt gccggtcgcg
      121 gtgaccgagt cgccttcggc gagggtggtc aggaccgagg cggaggtgct ggccgcgtcg
      181 cggacgttga ggacgtcggt attgaccagg agcgagccgc tgccagtggt tgtggtcgtg
      241 tcagtcgtcg ccgcgctcag cgcgccgtag gccaggaagg ggcgagcagc ccagctggtg
      301 acgccgtcga cgtcgatctg gtaccaggcg tagccgtcgg catcgaccgg accagccagg
      361 accgtaccga ccgcgccggt gccgaggacg tcggtgacgt tgccggcggt ggtcgcggca
      421 tcgcgcaggt tgagggcgtc ggtgacgact gtgaccgtgt cgccgaccgc gaatgagccg
      481 gtcgaggcgg cgtcggccag gaactcgccg gcgacgtaac cgctcaggtt gtcgaaagag
      541 acgaggtacc aggtgtagtc gccgtcggca attgggccgt cgaggatctc ggcgaaggcg
      601 ccctggggaa ggacctcgag tgcgtcggag gcggtaccga catcgctacg gagggtcagg
      661 ccatcggtgt tgacgacgac cgtctggccg gcggtgaagc cggtcgcatc ctgggcctgg
      721 gcggtgaaga ggccggagct ccagccggcg ccgagggcga ccagggcgat ggtgactgcg
      781 acgatgagag gtctgactgc tttctgcgcg ttcacgggac gtgtccttgc cgtgttcccc
      841 ggtggtccgg ggccaatcca tctgtctacg gttccagatt agggagggga aatcggaggg
      901 tcctgagcgt ttcctgtgaa tcgtctggga gtgccctcga gccacacgca cgtgatccgc
      961 gtgaatctgt cggggagctg ttacggggct ttggtggcgg tggggagcgg ggttggtgga
     1021 gtgaagccgg ggattccggt gaagggggtg accgggatgc gagtcgggtc ttcctgccat
     1081 gggagcgagc cggtggcgct ggcgaggtag gcggcgaagg caatgacact gatcatggcg
     1141 atcagggcga cggcggcgat cacgctggcg aagggatggc gcttgatccg ggacatgggc
     1201 tctggtcctt acgtgagatg tgccgggatg gcgactggct ggatccccat cccggcgggg
     1261 cgaaatcagg cggacttggt cgcgacgatg tcgacctcga gcgtgacgtg atggtcgacg
     1321 ctgaggacgg gtccgacgcg cggctcctcg atgttgtagt cctccatatt gaaggaggtc
     1381 gagccggagc cggtcagcgt gtcgccgtcg agcttggccg tgatgttcca gcggaccagc
     1441 ttcgtgacgc cgtgcatggt caggttgccg accagcgaga atgaggtctc ctggccgtcc
     1501 tgcaacggct gatccagccc ctcgaccgag gtcaggacga aggtggcaac cgggtaggtg
     1561 tcggtctgga gggtgttgcc gcgcaggtag ttgtcgcggc gggactcgtc gctggtcagg
     1621 gtgcggagat cgacatccca gcgagagcag gcgagcggca tgccctggtc gtcgaagtag
     1681 atctggccga cgatcgagtt ggtctggccg accgcttcgt tcgcgccctt gttggccagc
     1741 tcctcctggg ccttgtagcg cgcggccgag tcggcggtca gggtccaggt cgtcgcgggc
     1801 gacgtggccg ggatcggcgt cgcatcgctg ccgtcggcgg cgctgacgtt ctcgtcgcag
     1861 gcgatataga ccggattctg gtcgaggacg ccggcctcgg gcgtgctttc cggcgtgctc
     1921 tgatcctggg cgatggcgat attggcagcc agcccgagca tcagggccaa gagcaaggcc
     1981 ggcccggcgg tccaacggac catccggtgc aacagggcgg atcgggttcg catgcgtgct
     2041 tcctcctggg aacgattggt gtgcctcaac gctccggtcc ggccgcagcg catggttatc
     2101 ctctggtacg aagttgaacg tgcactggtc atttcctgtg ggttcgctgt gaatcaccga
     2161 ccggattcaa caggcgcgct ttctcaggaa actgacagga tcggttcagg attgtcacat
     2221 gttcggcgcg caggatggag acccgcgagc cggcacgcgc cggccagaac gatggagcga
     2281 tgaaaatgaa tcgaactact gcgcctggca atgaccatga tgaggggacc gatgtacagt
     2341 cgcggcgggt cgtcctcaag gcgggtgcga cggcgctcgg gatgagcacg ctggggatcg
     2401 gcgcgcttgg agccgccgcg cagagcgaca gcacgacgcc gaccaacccg gccctggatg
     2461 cgacaccaac gagctgcgtc ctgaccccgg agctgaccca ggggccgtac tacctggccg
     2521 atgagctgat ccgggccgat atcaccgaag gacgggccgg tgtgccgctg acgctgcgga
     2581 tcggcgtatc ggacatcaac gcgtgtggtc cgctggcgaa tgcggcggtg cacgtctggc
     2641 actgcgacgc gcacggcttc tactcgggtg tcagcgccaa cagccccggc agtgacgcga
     2701 cccagcaaca gatcgacgag gcggccaagg cgacgttcct gcgcgggatc cagatgactg
     2761 acgaggatgg cttcgtcgag ttcaagacca tctaccccgg ctggtaccgg ggccggacca
     2821 tccacatcca catgatggtg gcggtcgacg gaacggccga gcagacctat gagggtggcc
     2881 acatcgcgca caccgggcag ctcttcttcg acgatgcgct gagcgacaag attttcgcga
     2941 ccgaggccta tacgggacgg cccgaagacg agcggacgct gaatgaggaa gacgggatcc
     3001 tcggcgacca cgacgaagag cccggcttca tgctcgccct gacgccgaac aatgaggagc
     3061 ggatcgagga tggtttcgtg ggcgtgatcg cggtcggggt cgatccgacc tcgacgccgg
     3121 ccgccgccgg tggtccgggc ggcggacagc cgggcggccc tccgccaaac ggcaaccgct
     3181 gagccaggga gtgcaggttt tgcctggaac cgatacgagg cgccatcagg agacccgtcc
     3241 gcaattcgga cggatctcct gattgggaga gaaaatgcgc tggcacgtac gcccgaattg
     3301 aatgtttgcg caggaaagtg gatgtagcgt gcgttttcgg gcagcagcgt gatcttcgac
     3361 gggatggcac gaatgggcta ggaaatatcc caatggtgcc aaaaaggggg accatttgga
     3421 cccgtgccgg catgtcatac gcggctatcg tacccgcaag ccattccggc ggttgtgcca
     3481 gccacacgat cgctgcccct ttccctcgag tggttttgct cagacgcccg gtgccatccc
     3541 accgggcgtc ttggcgtccg cgacatggac caacatcagg cggcgacttg ctcgaccggc
     3601 tcgaggaggt cgtcctcgat cacgtcgtca aggcggctgg tgcggggcat gcgctggcgg
     3661 tccgggtcga gctctcgctc ggtgtggagg gcgatcgcgg cggcgaagca ggccattagc
     3721 accatgccga cccatggcca ggtcggcccc agccagagca gcccggagac gatgctcggc
     3781 gcgatggtcc ggccgattcc ccacgagagc gagaagagcg ccaggtagcg gccacgcagg
     3841 gcggctggcg caagtgccga gggcaggcca tttgtctgcg gcgcgtggaa gaggtcgccg
     3901 ccgatggtga ccacgatcgc gaagaacatg tacgggatca gcaggctccg cggcacgatc
     3961 tgcgcgaagg cgaagacgat cgcgccgacg acccagatcg cggccgccag caaatagacg
     4021 cgggtccgca gccagccggt cacgacgcgc aggccgagcg attgaccgaa ggcgagcacg
     4081 atcgtggtcg ccacgaagag caggcccggc agccaggcag gcagatcgag atcgtcgacg
     4141 acgtagatcg gcatggccag accgccgatc aggactgtca tcacggcgac cgtgttggcc
     4201 actgccatct tcatgaccgg gcgatcgcgc accacagccc gccagccgcc gtggtcatgg
     4261 tggatcggct cgacctcggg cgagggcacc cgcaggtaga tgatgatggc cgcgatcgcg
     4321 aaggtgcagg cgttgagcaa gaccagcaac cggtagccgt cggtcccggc cagggcgacg
     4381 atcagcgcgg cgagcaatcc tccgacgccg aagccggcct cgcgggtcat gcccatgaag
     4441 gcgaaccagc gatcgcgctc gttgtccggg gccatgatcg agatgaagga ggggaagccg
     4501 acccagaaga tgcgctcgcc gagcacggcc agcgtggaag cgacgatcag gagcggaacc
     4561 gagcccttga cgaaaagcag gctgaagtac ccgaccgcgc agatgatctg gctggccagc
     4621 atcatccggc gggcgccgat cttgtcgacc aggctgccgg caatcgggtt cgaggcgagg
     4681 gccaggcccg acgccagtga caggaccgcg ccggcggtgc cgagactgaa gccggccgcc
     4741 ttggtcaggt aaagcagata aaaggtgagg aagaggccgc tgccgacggc atcgatccag
     4801 gtggcgacgt acagtgtgac ggcgccggga cgatcgagat gaggaaggcc gaagcgttcc
     4861 gcccaggagt tggatggtcg ccgggccggt cggtcagcgg aagcaggcat gtgccgttgt
     4921 ctcgcacgga ttggtaacgg tcaaggtgtg ccgggcgtgc cgcggccggg agattttgcc
     4981 acatcttgtg tagtccagat tcttgatctt ggtggtatga caaaggcagt cattctgtcg
     5041 cgttgattac cattgccctg ctgtgcagca tggagcgggt acctgctccg tctgcgccag
     5101 gaggtttccc aatgagcaaa aaacaggtcg cgcttgaagt gaccatgatg ctggctgccc
     5161 agcaactggt tgaccggtgg ttgcggtcgc cgggtcgtgg tccttttcag cgcgctgtaa
     5221 cccaggaggt acaagcacgg tggagcgaca tgccggggac ggaggcgcaa cgacgcgcat
     5281 tccggcgcct ggcgaacgcg atccgggtcg gggagggtca cgcgattgcc aacccagggc
     5341 cgctgctgaa gattgctcgg cagaacctgc gcccgtaaca gtccaaaccg tccagtagaa
     5401 tcgaggcacg tcaactgtca tccggttccg ccgagagggt cgggtttgat cctgggccga
     5461 gcccatcgtt tgcgttgcca accctgcaga cccaattcgg agtagacaac gtatgccccg
     5521 caccgtcctg atccgcctga ttggcgctgt actcctgctc gccgtgcagg cagcggattt
     5581 cgcttgcgtg ccggttcagg gtgttcacgc gcagtcgggt acgacgattc aggtagcgcc
     5641 gggccgatac atcgtactgc tcaagcccgc gaccggcgtg accagcgccg gagtcgcagc
     5701 cgggtacgat gcgcgaccgg gtgtcacggt cgatcacgtc tatacctcgg cgatcagcgg
     5761 gtttgccgga acgatcgatc gggcagccgc gcaggcgctg gtcaatgatc cgcgcgtggc
     5821 cggcgtcttc ccggacatca tctcgcatgc ggcgtcccaa tccctcccgg ccggcatcga
     5881 tcgcgtcgat gccgatctca acccgacgaa ggccgg
//
LOCUS       DATAOZ010000155         3872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6640543, whole genome shotgun sequence.
ACCESSION   DATAOZ010000155 DATAOZ010000000
VERSION     DATAOZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6640543"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1939)
                     /locus_tag="VHR64_04250"
     CDS             complement(287..1939)
                     /locus_tag="VHR64_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302069.1"
                     /translation="MSAMCRNRFLSAAATVLVLLALVGGSPGLVHAQDYSGWYGPFDD
                     GCYYWWDGYQYTGDVDCNGDGYADSAQQSAAAGWYDAYGDGCLYWWDGTQYTGDVDCN
                     SDGYADQSQQSSYQAGWYDANGDGCLYWFDGTQYTGAVDCNGDGFADSAAAGYAAGWY
                     GPAADGCNYWWDGAQYTGAVDCDGDGYDDAAQQSYQPGWYGPATDGCSYWWDGSQYTG
                     DVDCDGDGYADVAQVSSQAGWYDYYGDGCLSYFDGTQYTGDSTCSQDSGSGYGAGWYG
                     PFADGCNYYWDGTQYTGEQDCGATAAQEYVAGWYGPFDDGCSYEWNGAAWTGAASCPD
                     TDVTAGTASPAGWYGPYDDGCYYEWGGTTWTGNATCSAGTTYAFPSGWYGPASDGCSY
                     YWDGARYTGDVQCASAGTPAGWYGPYADGFLYEWNGSAWTGAKSYMADGDTQIVIPAP
                     ATTVVPTTMSTTGGQSILAGNDVTITIPPNTGSCGDFCNSYTSLMDAWTKMMQQNRSA
                     GVLPSSSTPTIYPRNPLPSECFAYNYNTDQCFYNPYDGRFGT"
     gene            complement(2049..>3872)
                     /locus_tag="VHR64_04255"
     CDS             complement(2049..>3872)
                     /locus_tag="VHR64_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3302070.1"
                     /translation="SHRLRLLTSDTHMVPDRHRTLYAAVAWTYDLLDPADQRMFRMVS
                     VYPGPFTLDAAEFSFGPDALEQLTRLVDFNLIVPVDDLPDSPRFRMLETLRAFGLEQL
                     DAHDERESAQSLAANWMRHRAAHWRSEDRARESQLVHVPFQRVKTELAAIDLALDYFA
                     STENLDAVLEVAVASADAWNTWGRFDQSVRWLTLGLSTTTAPAELRGRALIALGTALH
                     LLGRHGEEAVILSQAVDIFADGEEPMFLGSALYRRGLLAYSQGHYADARIDFEQSIAL
                     SAENDLAVLAAENVLAFVLLDLKDWDAAAAIFRRHAEVGVTTGDLWTEAIATHNLACL
                     AMERGRDHEAYDLFAAALPLYEAVADRDGLSQTLSLLGLVAVRIGRADEGFTVLRRGR
                     DIASADGLRWAESVAQHAMGDAYLTRGDIGEAESHFRSALALRHELGVRLGMAETLLA
                     LGRSTVASGDEQAGAHLIGAADGIRERMGLPVESSGGGDTDLTAFRHDRQYAAAYAAG
                     RHLGDDEAVGLGLQPPRRSASQTTPTVPTDGHQRSPHDLTGRELDVLRLVAQGKTNAE
                     IGDLLFISPFTAKTHIANLLGKLMVENRAAAAAWAVQQGLV"
BASE COUNT          694 a         1257 c         1228 g          693 t
ORIGIN      
        1 tagccgccga accactgtgt atagggctca gcacagaccg gctgacgcgc gtatccgtct
       61 ctcccggtgc cagccggtgg cacgtgcaga cgatacaggt tcggcggatc gggatgacaa
      121 ccggcgcgag cgttgggatg ctgttgggcc gtcgggcgca cccaatacca aacgccgctg
      181 ccgtacatcg ggccgaccga cggggcaccc agggaggacg caatagtctg ggtgccccgt
      241 cggctgaata ttccgctcgt cagagcgggc ctgtgcatca gaccagtcag gttccgaagc
      301 gaccgtcgta cgggttatag aagcattgat cagtgttgta gttgtaggcg aagcactccg
      361 aggggagggg attgcgcggg tagattgtcg gcgttgatga gctcggcaag acaccggcac
      421 tcctgttctg ctgcatcatc ttggtccagg cgtccatcag gctcgtgtac gagttgcaaa
      481 aatctccgca gctcccggtg ttcggcggga tcgtgatcgt aacgtcgttg cctgcgagaa
      541 tcgattggcc accggtcgtg ctcatcgtgg tcggcaccac cgtcgttgcg ggcgccggga
      601 tcacgatctg ggtgtcacca tcggccatgt aggatttcgc gccggtccag gccgatccat
      661 tccactcgta cagaaagcca tcggcgtagg ggccatacca gccggcaggg gtcccggcgg
      721 atgcacattg cacgtcgccc gtgtaccgcg ccccgtccca gtaatagctg cagccgtcgc
      781 tcgccgggcc ataccagccg gaggggaagg cgtaggtcgt gcccgcgctg caggtcgcgt
      841 tgccggtcca ggtcgtgccg ccccattcgt agtagcagcc atcgtcatac ggtccatacc
      901 acccggcagg ggatgctgtg cctgcggtga cgtctgtatc cgggcagctg gccgcgccgg
      961 tccacgccgc gccattccat tcgtacgaac agccatcatc gaacgggccg taccagcccg
     1021 ccacatattc ctgggctgcc gttgcgccac aatcctgctc accggtgtat tgcgtgccgt
     1081 cccagtaata gttgcacccg tctgcaaagg ggccgtacca gcccgcaccg tacccggagc
     1141 cggaatcctg ggagcaggtg gagtcgccgg tgtattgggt cccgtcgaag taggaaaggc
     1201 agccatcacc gtagtagtcg taccaaccgg cttgggagct gacctgcgcg acatccgcgt
     1261 acccgtcgcc atcgcagtcg acgtcgccgg tgtactggct gccatcccac caatagctgc
     1321 atccgtcggt tgccgggccg taccaccctg gctggtagct ctgctgtgcc gcgtcgtcat
     1381 acccgtcgcc atcgcaatcg actgcgcctg tgtactgcgc accatcccac cagtaattgc
     1441 atccatcagc ggcaggtcca taccagccgg cggcgtagcc ggcggcggct gagtcggcga
     1501 agccatcccc attgcagtcg accgcgccgg tgtactgcgt gccatcaaac caatacagac
     1561 acccgtcgcc attggcgtcg taccaccccg cctggtacga gctctgttgc gattggtcgg
     1621 cgtagccatc actgttgcag tcgacgtccc cggtgtattg cgtgccgtcc caccagtaca
     1681 ggcacccgtc gccataggcg tcataccagc cggccgccgc cgattgttgc gcggagtcgg
     1741 catagccgtc gccgttgcaa tccacgtcgc cggtgtattg atagccatcc caccagtagt
     1801 agcagccgtc gtcgaatggg ccataccagc cggagtagtc ctgggcgtgg acgagccctg
     1861 gggaaccacc caccagggca agcaacacca gaaccgtggc cgccgcgctc aagaagcggt
     1921 tccgacacat tgcgctcatc tcgcgtctcc tcacaaccct cgtcatcccg accatccggt
     1981 gtcgaaagca tcgctgcgga gccggatgag gacctcgccc gatcggagta tttgtgcgga
     2041 gtagtcggtc agaccaagcc ctgttgtacc gcccaggcgg ccgcagcggc gcggttctcg
     2101 accatcagtt tgccgagcag attggcaatg tgggtcttgg cggtgaacgg gctgataaag
     2161 aggaggtcgc cgatctcggc attggtcttg ccttgggcga cgagccgcag cacatcgagt
     2221 tcgcggccgg tcaggtcatg cggggatcgc tggtgaccat cggtcggtac cgtcggggtg
     2281 gtttgactgg cactgcgccg cggtggctgc aagccgagtc ccaccgcctc atcgtcgccg
     2341 aggtggcgac ccgcggcgta ggctgcggca tattggcgat cgtgccggaa cgcagtcagg
     2401 tcggtgtcgc caccgccgga cgactcgacg gggagtccca tgcgctcccg gatgccgtcg
     2461 gccgcgccga tcagatgtgc gcccgcctgc tcgtcgccgg aggcgaccgt gctccgaccc
     2521 agcgcgagca gcgtctcagc catccctagt cgcacgccca gctcgtgtcg aagtgcgagc
     2581 gcagatcgga aatgggactc agcctcgccg atgtctccac gggtcagata cgcgtccccc
     2641 atcgcatgct gcgcgacgct ttccgcccac cgcaacccat ccgcggatgc gatgtcgcgt
     2701 ccacggcgga gcacggtaaa tccttcatca gcccgtccaa ttcggaccgc gactaaccca
     2761 agcagcgaca acgtctgcga caggccatca cgatcggcga ccgcttcgta gagcggcagt
     2821 gcggcggcaa agaggtcgta cgcctcgtga tctcgtcctc gctccatcgc caaacaggcc
     2881 aaattgtgtg tggcgatcgc ctcggtccaa aggtcgccgg tggtcactcc gacctcggcg
     2941 tggcggcgga agatcgctgc cgccgcgtcc caatccttga ggtcgagcaa cacgaatgcg
     3001 agcacgttct cggcggcaag cacggccaga tcgttctctg cgctgagcgc gatcgattgt
     3061 tcaaaatcga ttcgggcatc ggcgtagtga ccttgactgt aggcaagcag cccgcgccgg
     3121 tagagagcgc tgccgaggaa catgggctcc tcgccatccg cgaatatgtc cacggcctgg
     3181 ctgaggatca ccgcttcctc gccgtgccga cccaggagat gcagggcggt accaagcgcg
     3241 atgagcgctc ggccacgcaa ttccgccggg gcagtggtgg tgctcaggcc aagggtcagc
     3301 cagcgcactg actgatcgaa gcggccccag gtattccagg catcggcact cgcgacggcg
     3361 acctccagca cggcatccag attctcggtg gaggcaaaat aatcgagcgc gaggtcaatt
     3421 gcggccagtt cggtcttgac gcgctgaaac gggacatgca caagctgtga ttcacgcgcc
     3481 cgatcctccg atcgccagtg tgcagctcgg tgtcgcatcc agttcgccgc caacgactgg
     3541 gccgattcgc gctcatcatg cgcgtcaagt tgctcgagcc cgaaggcacg gagcgtctcc
     3601 agcattcgga atctcgggct gtctgggagg tcgtcgactg ggacgatcaa gttgaagtcg
     3661 acgaggcgag tgagctgttc gagcgcatcc ggtccgaaac tgaattcggc ggcatcgagg
     3721 gtgaatgggc ccgggtacac actcaccatg cggaacatgc gctgatcggc cggatccaag
     3781 agatcgtagg tccacgcgac cgccgcgtac agcgtgcggt gacgatccgg gaccatgtgc
     3841 gtgtccgagg tcaagagccg caggcggtgc ga
//
LOCUS       DATAOZ010000156         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7168274, whole genome shotgun sequence.
ACCESSION   DATAOZ010000156 DATAOZ010000000
VERSION     DATAOZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7168274"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VHR64_04260"
     CDS             <1..1197
                     /locus_tag="VHR64_04260"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX3302071.1"
                     /translation="LDSGNIDFDVNLIVSDGATDQDGQYFFDVFTTDGFNGDIPLVNF
                     AYAPVMEVLPRKYRFRILVGSMSRYWKLAFADPSGNAVAIRQIANDGNFLVNPVLLTA
                     LDEQGVAERYDVIVDFSRFRVGDKIKLVNLLTMRDDGRGPAGAVTLAQALAGVATDPC
                     IGPIMEFRVASSVPSVDAPGQVLTTANSCGVNDKSQVPLTLTQQIPVVTPVRTRTIEW
                     GRAGIGDSRDPVTGQCTPDCPENMPFPWTVKINGQEAHSFNANRISELIPKPGEIEHW
                     TYINGGGGWDHPIHLHFEEGVTMNRGGAPIPPTELNVRKDVWRLRDAGRVQFQIQFGE
                     YGGSYVNHCHNTVHEDFALLMRIQLLSAAGPQVAVTPTPNPTPDGVFFTTPEILPEGD
                     PSGSGA"
     gene            complement(1303..2667)
                     /locus_tag="VHR64_04265"
     CDS             complement(1303..2667)
                     /locus_tag="VHR64_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3302072.1"
                     /translation="MTDVGSGPRSSLARRLSIRIAIVTLATSLVQLSVIMWALNVDYD
                     HLALDHVRREANWILRGIKPGPGKLTFELPRDMGHYDERNQDNYAFRVLDRGGQVIAS
                     KRAPILERTSPWHPASREEIPSFWFRELDDTRPFYFAGGQRFQIGDSDVLIEVAIQGD
                     PAGIHRWILFYEALEDVWLPIVPFTFLIPLAALFASRGALDFLTRAAQQAERVDPANP
                     AQSIDFAEIPREAAPFAAAINRLLLRVSTLMQSYRVFMGCAAHELRTPLAAMLMELEK
                     IDDPRSRLLEKDVLGMSESVNRLLTLIRLQCVESPDLVEVEVGAVVWDTVESLRMWAR
                     AHDHEIDLDLQEPGTVRGDPVAMREAIRNLVENAVKHTPAGTPVRVTVRSGCMITVED
                     GGPGLPAQGLDRLFEPFHRGSQCKEGVGLGLTIVRRAVDLHHGSIEVGQSPLGGAMFH
                     VRFA"
     gene            complement(2664..3341)
                     /locus_tag="VHR64_04270"
     CDS             complement(2664..3341)
                     /locus_tag="VHR64_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3302073.1"
                     /translation="MRLLLVEDNVRLRQSISDTLRHGGYVLDAFGTVAESHAASQQIV
                     YGLLIVDLTLPDGDGLKLIRELRSANCSTPILVITARTAIDDRVKGLDSGADDYLVKP
                     FHQAELVARVRALLRRPRDIRAATIRAGGVVLDETTGDVRVNGEVLGLRPRERCLLAL
                     LLRRAEMTVPKSAIESALSEFDREISPNAIEVLVCRLRKALSENPTSIVIETVRGIGY
                     ALREVKQ"
BASE COUNT          710 a         1086 c         1059 g          645 t
ORIGIN      
        1 ctggactccg gcaacatcga tttcgatgtc aacctcatcg tctccgacgg tgccaccgac
       61 caggatggcc agtacttctt cgacgtcttc accaccgacg gcttcaacgg cgacatcccg
      121 ctcgtcaact tcgcctacgc gccggtcatg gaggtgctgc cgcgcaagta ccgcttccgc
      181 atcctcgtgg gaagcatgtc gcggtactgg aagctggcct tcgccgatcc gagcggtaat
      241 gctgttgcca tccgacaaat cgccaacgat ggcaacttcc tggtgaaccc ggtgctgctc
      301 acggcgctgg acgagcaggg tgtcgccgag cgctacgatg tcatcgttga cttctcccgg
      361 ttccgggtcg gcgacaagat caagctcgtc aacctgctga cgatgcgcga cgacggccgc
      421 gggccggcgg gggcggtgac gctggcgcag gcgctggccg gggttgccac cgatccgtgc
      481 atcggcccga tcatggagtt ccgggtggcg agttcggtgc cgagcgtcga tgcgccgggg
      541 caggtgctga ccacggccaa cagctgcggc gtcaacgaca agagccaggt gccgctgacg
      601 ctgacgcagc agatccccgt cgtcacgccg gtgcggacac ggaccatcga gtggggccga
      661 gcgggtatcg gcgactcgcg cgaccccgta acgggtcagt gcacgcccga ctgcccggag
      721 aatatgccgt tcccctggac cgtcaagatc aacggccagg aagcgcactc cttcaacgcc
      781 aaccggatct cggagttgat ccccaagccc ggcgagatcg agcactggac ctacatcaac
      841 ggcggcggcg gatgggacca tccgatccac ctccatttcg aagaaggtgt gaccatgaat
      901 cggggcggtg cgccgatccc gccgaccgag ctcaacgtca ggaaggacgt ctggagattg
      961 cgggatgctg gccgcgtgca attccagatc cagtttggcg agtatggcgg gtcgtacgtg
     1021 aatcactgcc acaataccgt gcatgaggat tttgcgttgc tcatgcgcat ccagctcctg
     1081 tccgccgctg gcccgcaggt cgcggtgacg ccgacgccga atccgacacc cgacggtgtc
     1141 ttcttcacca cgccggaaat cctgccggaa ggcgatccga gcggttcagg cgcatagtcg
     1201 aagactgaga agcacgcgcc gaagggtaga cggcaagacc cttcggccgc atccgagaga
     1261 ttgcgccgct gcagcgagcg gctgcggttc tgcgactgtc tctcaggcga agcgtacgtg
     1321 gaacatggcg ccgccgagcg gcgattgacc gacctcgatc gaaccgtgat gaaggtcaac
     1381 cgctcgccgg acaattgtga ggcccaaccc gacaccctcc ttacactggc ttccccggtg
     1441 aaatggctcg aacagccggt ccaagccctg agcgggaagt cccgggccac cgtcctcgac
     1501 agtgatcatg catcctgacc tgacggtgac gcgtaccggt gtacccgctg gcgtgtgctt
     1561 cacagcgttt tcgacgagat tccttatggc ttcacgcatc gcgacggggt cgccgcgcac
     1621 cgtaccgggc tcctgcagat ccaggtcgat ctcgtgatcg tgggctcgcg cccacatgcg
     1681 caaagactca acggtgtccc atacgacggc gccaacctcg acctcaacga ggtcaggaga
     1741 ctcgacgcac tggagacgga ttagagtcaa cagccggttg acgctctccg acatgcccag
     1801 aacatccttt tccagaaggc gtgaccgtgg gtcgtcgatc ttttcgagtt ccatcagcat
     1861 ggcagccaac ggggtacgca gttcgtgagc agcgcaaccc atgaagaccc gataggactg
     1921 catgagcgtg ctgaccctca gtagaaggcg gtttatggcc gcggcaaaag gtgccgcttc
     1981 cctcgggatc tccgcaaaat cgatcgactg ggcgggattt gccggatcga cacgctccgc
     2041 ctgctgagcc gctcgtgtca ggaagtcgag cgcgccccga gacgcgaata gcgccgcaag
     2101 cggtatcaag aaggtgaagg ggacgattgg gagccaaaca tcctcaagag cctcatagaa
     2161 caggatccaa cggtgtatgc ccgcggggtc gccttgaatg gcgacttcga tcagtacatc
     2221 ggagtcgcca atctggaatc gttgcccacc agcaaaatag aatggacggg tatcgtcaag
     2281 ctctctgaac caaaaggagg ggatctcctc acgagatgca gggtgccagg gggatgtgcg
     2341 ctccaatatt ggcgcccgct tggaggcaat cacctgccca ccgcggtcga gaactcgaaa
     2401 cgcatagttg tcctgatttc tttcatcgta gtggcccata tcccgcggaa gctcgaaggt
     2461 cagctttccg ggacccggtt tgatcccgcg cagtatccaa tttgcttcgc gtcgcacgtg
     2521 atccagagcc aaatggtcgt aatctacatt gagcgcccac atgatcaccg acaactgaac
     2581 aaggcttgtc gccagcgtta cgatagcgat gcgaatagaa agccgtcgtg cgagactact
     2641 gcggggccca gaaccgacat cggtcattgc ttgacttccc tgagtgcgta tccgatgcct
     2701 cttactgtct cgattacgat gctcgtcgga ttttcgctca gagcctttcg caggcgacac
     2761 acgagcactt cgatggcatt aggggagatt tcccggtcga attcggacaa cgcggattca
     2821 attgctgatt tcggcactgt catctctgct cgacgaagga gaagggccag caagcagcgc
     2881 tcacgtggcc gcaaaccgag aacctccccg ttgacccgca cgtcacctgt tgtttcgtcg
     2941 agcaccacac cccccgcccg tatcgtcgca gcgcggatat cccgaggtct gcgcaagaga
     3001 gcgcgaaccc gcgccaccag ttcggcctgg tgaaagggct tcacaagata gtcatcggcg
     3061 ccggagtcca atcctttcac gcggtcgtcg attgcagtcc gggccgtgat cacgaggatc
     3121 ggcgttgagc agttcgccga gcgcaactct ctgatgagtt tgaggccgtc gccgtcagga
     3181 agggtgagat cgacgatgag caggccatac acgatttgct gcgatgcagc atgggactcg
     3241 gcaacggtgc cgaaagcatc cagcacgtag ccgccatggc gaagcgtgtc gctgatcgac
     3301 tgccgcaacc gcacgttgtc ctcgaccagc agtagtctca tgcacgcctc accacggcga
     3361 tcggctcccc ccggtgcatg gacatgatgt cacccagtgg ccccggccgc ggcctcctgg
     3421 cagtaatggc gacccctgaa aggttgctgc aaggatggct tgccatagta tgggccgtag
     3481 gtcgtagata taagtgtgat
//
LOCUS       DATAOZ010000157        10315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2696458, whole genome shotgun sequence.
ACCESSION   DATAOZ010000157 DATAOZ010000000
VERSION     DATAOZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2696458"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VHR64_04275"
     CDS             <1..321
                     /locus_tag="VHR64_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3302074.1"
                     /translation="HRAVGLSAHDAAYPSARDPHAFPEHLAEGLQPHKTAEVWYFNAE
                     HPDLIVDITAVMDKKIESLKAHFSQVGASDDVWDRVRDRAREVAQDQPFEFGEAYKVA
                     QLRR"
     gene            318..1535
                     /locus_tag="VHR64_04280"
     CDS             318..1535
                     /locus_tag="VHR64_04280"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEX3302075.1"
                     /translation="MTKVPGEVARQILSTGKAAFGDEWFPERYVGAPGRIEVIGNHLD
                     YNGGPVLCAAIDRWIVVGLREASNDGPIRAVFANMSGSLISEVGADLSEWRSERGAPY
                     PVHYLRGVIASLNAWSVGVKPGVELVVAGDLPHGIGISSSAALCVALVQALSVDLPSP
                     PEIVLIAQEAEHRAGSPAGTMDQSASVFGGLIRFEGSTGAVERISADLTGLRFVVVNS
                     GVVRNLATSSYPTRVRETQNALALLQDAWDQELPALGAIGVSDLSRAEGILTTDDEPV
                     LARRVRHVVTEGQRVRDAEQAILASDWATVGRLMTESGRSSATDYDVSHPQVERIVSI
                     CLELPGVLGARMMGGGQGGSVLVLIEEDCFGELADRLDRDYFAGLTEQPQVKLNWCVF
                     APGAGALEPPQAS"
     gene            complement(1523..1846)
                     /locus_tag="VHR64_04285"
     CDS             complement(1523..1846)
                     /locus_tag="VHR64_04285"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEX3302076.1"
                     /translation="MAERQLESVSIHASQRLAQRNLSSRDIEYVLNHGRRIRNGKAIF
                     ICLGRRDIPAADQRHSKLTRLEGTVLVLDPMTGSHLTTAYRNRRSGIRDVKRKQKHSL
                     PRYEA"
     gene            complement(2036..3070)
                     /gene="moaA"
                     /locus_tag="VHR64_04290"
     CDS             complement(2036..3070)
                     /gene="moaA"
                     /locus_tag="VHR64_04290"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX3302077.1"
                     /translation="MVATAETKPITLFAPGKGVAKDAYGRTMSYLRISLTDRCNFRCV
                     YCMPEMGMKFQPRDEILTDDELIRVVGAFAELGFTKFRLTGGEPTIRPHLVDIVRAIK
                     SFPGVEEVTMTTNALLLGRMAEGLAEAGLDRINVSLDTLNPERFKAMTRGGRFDMVWD
                     GVLAAERAGITPIKINTVVVRGQNEHEVAELARLTIDRPWQMRFLEIMPMEGVGMVYD
                     EGLVTSEETQARLEYEFGPLEHIASPPGDPARVWKIPGAAGTIGFISPISEPFCSTCN
                     RVRLSADGKLRLCLLRADEVDLRDIVRNGGTDDQLTGRIRSAIWRKPWGHGIAEGDFN
                     IGRGMSQIGG"
     gene            3179..3721
                     /locus_tag="VHR64_04295"
     CDS             3179..3721
                     /locus_tag="VHR64_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HEX3302078.1"
                     /translation="MSQTEYVPQGALSSSSLRALILSDRPLIRDYRNLDTQLQPNGFD
                     LTLESVAIHHGAGVVGQSNEDRVLPDLVPLEFDSRGWVDLQPGIYHITYNEVVALPLN
                     LMALGRPRSTLNRIGVTIHTAVWDAGYEGRSTSLLSVMNPNGVQLQRDARVMQLVFFG
                     VSIPPEIGYSGVYQGENIGR"
     gene            complement(3718..3987)
                     /locus_tag="VHR64_04300"
     CDS             complement(3718..3987)
                     /locus_tag="VHR64_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302079.1"
                     /translation="MARILDIVFRRQVVEPPIPICPDHKVEMHLRGKMGRPTRFTDMS
                     QETYTVIYVCPVIDCDQSKEVQQVRSQIAVPGKPPRRPDYSRRGH"
     gene            4107..4529
                     /locus_tag="VHR64_04305"
     CDS             4107..4529
                     /locus_tag="VHR64_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302080.1"
                     /translation="MVDDIVKMLVERYGDTWTSDEEANLRFSLEAEHVLGNLEGVHLI
                     GDCDLEDGRITLNVWVDEPIYDLMGADQLAYDIFGRLSEEIFYAERRFDEKGIRYPFV
                     TGSLRHGHVGQLLLQGPHAAEFADRHQLRIRGDVRFHA"
     gene            4541..4909
                     /locus_tag="VHR64_04310"
     CDS             4541..4909
                     /locus_tag="VHR64_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX3302081.1"
                     /translation="MHKVTVEGIETVVTAGDGRRLVLAIEDAGIDILHRCGGNARCAT
                     CRVEVVDGEPSPITPAEEERLQRVTGRKPNTRLSCQIQVRNDLTVRVLRRLSENPELG
                     DSGPRPIEWPSDRPLPPEED"
     gene            4969..5231
                     /gene="ffs"
                     /locus_tag="VHR64_04315"
     ncRNA           4969..5231
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHR64_04315"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5305..5793)
                     /locus_tag="VHR64_04320"
     CDS             complement(5305..5793)
                     /locus_tag="VHR64_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302082.1"
                     /translation="MDNPISWDYLSTVPGPNEVFGPLAILYLVFFVVGFLFSLAVYNN
                     QGRRLFPNPVIFRLVRRWAGWTSVIFGAGIFFFLIRALQINPFTFAMRFWMWVTVLAL
                     GVVFVLLLVDIKRRYKVELAEWESRKQRDQYVRPLSNVAGRKQAHAALATNPRPVRRR
                     RR"
     gene            complement(5856..6557)
                     /locus_tag="VHR64_04325"
     CDS             complement(5856..6557)
                     /locus_tag="VHR64_04325"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX3302083.1"
                     /translation="MQQVTLRIKRYNPEVDLKPHFEEYRVDVEPTDRVLDALNKIKWF
                     EDGTLTYRRSCAHGICGSDAMRINGRNRLACKVLMGEFGQKITLEPLIGFHVVKDLVV
                     DMDRFFDAYKSVKPYLIADDAPVGRERYQSPVDRDRFEDTTKCILCAACTTACPVSWT
                     SEEFVGPAAIVNAHRFIFDSRDEGAAERLAILNKGGGVWRCRTVFNCTEACPRGIKIT
                     KAIEEVKRALILNRV"
     gene            complement(6582..8312)
                     /gene="sdhA"
                     /locus_tag="VHR64_04330"
     CDS             complement(6582..8312)
                     /gene="sdhA"
                     /locus_tag="VHR64_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX3302084.1"
                     /translation="MGYHRYDAVIVGAGGAGLMAAIQMAGKANVAVVSKLYPTRSHTG
                     AAQGGISAALGNTEEDHWEWHMFDTVKGGDYLVDQDAAEVLAREAIDAVLELEHMGLP
                     FNRTPDGKIDQRRFGGHTKNFGESAVRRACYAADRTGHMIIQTLYQQGIKHDVHFFDE
                     HYMTDLLFAEDGRCCGVVTRNLSTGEVNTLHSKCVLIATGGFGRVFKITSNAHAGTGD
                     GVAIAYRRGVPLMDMEFYQFHPTGLYKLGILLSEAARGEGGILRNSEGEAFAARYAPT
                     LADLAPRDMVSRFIYQEVKEGRGIDGKDYVLLDLTHLPESVIDEKLPDITDFARTYLG
                     VEPKKEGVPIQPTAHYAMGGLPTNIEGQVLLNEQGALIPGLYSAGESACVSVHGANRL
                     GTNSLVDLVVFGRRAGRHMLEYIKTAELATLPAEPELATNAELSGVLGRAKGESIAEI
                     RERMQVMMTDKVSVVRNREGLTEAQQELGQLRDAYNHSAIQDKGSIFNTDLMEALELG
                     YMLDCADTIVGGALLREESRGAHYRSDFEARDDKNWLAHTMVRKTSGGLTYAKKPVSI
                     TRFEPKERKY"
     gene            8504..9688
                     /locus_tag="VHR64_04335"
     CDS             8504..9688
                     /locus_tag="VHR64_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3302085.1"
                     /translation="MSLSQTDSPIWQDAALVRRALEVGAQFADKVIETARVIAMVAAP
                     TNDERDRAVLVADLFQTHGASDVSIDSIHDVVGRLKGTSSEKALLLAAHTDTVFSRET
                     VLKIHQDDQRAYGPGLGDNSLGVASVVLLPDMLQALGVTPATDILLTGNVGEEGLGNL
                     RGITQVLTDHADVGAVIAVEGQNLGRITHIAVGSVRLRVTVTGPGGHSWGDFGNPSAI
                     HAAARLIEQLDRIPLTQSPKTTLNVGMISGGISINSIAPKVTFDVDLRSTDATALKRV
                     VDRVYAALASRDERITVEPEVLGKRPAGQVAVTSRIVVAAAEILRSLSYSPAADASST
                     DANAAIAMGLPAVCIGLTTGGNAHRLDEYIDLAPVKTGLAQLMLLSLAVSNDLAQGRL
                     SP"
     gene            9690..>10315
                     /locus_tag="VHR64_04340"
     CDS             9690..>10315
                     /locus_tag="VHR64_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302086.1"
                     /translation="MPDFTDDLLFPAGTWAAVGTGDDRHGNTLRVLLFDGPIPVDDRT
                     GPAPDVFDFVDMQCGIGLEFAAVSSSAWLGRYAGDDDAIDLSPYVALDAWSGIIGIQP
                     RLIAPGVEEVAARLGEVVARLGALDPGVLLRSDAYLGFRRNGEQRLIRAALAHSQATH
                     GAASPRPPELAFLTNREKPERLKRVKNSDRVEPDEIAASFDIGGPISKR"
BASE COUNT         1860 a         3202 c         3294 g         1959 t
ORIGIN      
        1 catcgcgcgg tcgggctttc ggcgcacgac gcggcctatc catcggctcg cgatccgcac
       61 gcctttccgg agcacctcgc cgagggcctg cagccacaca agacggccga ggtctggtat
      121 ttcaatgccg agcatccgga cttgattgtc gatataacgg cggtcatgga caagaaaatc
      181 gaatcgctca aggcgcactt ctcgcaggtc ggcgcgagcg atgacgtttg ggaccgggtc
      241 cgggatcgcg cacgcgaggt cgcccaggac cagccgttcg agttcggcga ggcgtacaag
      301 gtggcgcagc ttcggcgatg acgaaggttc ccggagaagt cgcccggcaa atattgtcga
      361 ccggaaaagc ggcgttcggt gatgagtggt ttcccgagcg gtacgtggga gcgcccggcc
      421 gcatagaagt catcggcaat cacctcgact acaacggcgg ccccgtgcta tgcgcagcga
      481 tcgatcgctg gatcgttgtc ggcctgcgcg aggccagcaa cgacggtccc atccgggccg
      541 tcttcgccaa catgtcgggg tcgctcattt ctgaggtagg ggctgatttg tcagaatggc
      601 gctcggagcg gggcgcccca tacccggttc attacttgcg cggtgtgatc gcgtccctca
      661 acgcgtggtc ggtcggggtg aagccgggag ttgagctggt cgtcgcgggc gatctgcccc
      721 acggcatcgg tatcagcagc tcggctgcct tgtgcgtcgc gctggtgcag gcactctccg
      781 tcgatctccc ttcgccgccc gagatagtct tgatcgcgca ggaagcggaa caccgggcag
      841 gatccccggc cgggacgatg gatcagtcgg ccagtgtctt cggcggactg atcaggttcg
      901 agggatcgac tggagccgtc gagcggatct ccgccgacct taccgggctc cgattcgtgg
      961 tggtgaattc gggcgtcgtc cgcaatctgg cgacgtcgtc ctacccaacg cgcgtccgcg
     1021 agacccagaa cgccttggcg ctgctgcagg acgcctggga ccaggagctc ccggcactcg
     1081 gcgcgatcgg cgtttctgac ctttctcgcg cagaaggaat actcacgact gacgacgagc
     1141 cggtactggc ccgacgggtc cgccacgtcg tgaccgaggg ccaacgcgtg cgcgatgccg
     1201 aacaggccat ccttgcctca gattgggcga ccgtgggacg gctgatgacc gaaagcggac
     1261 gctcctcggc aaccgactat gacgtttcgc acccgcaggt cgaacgaatc gtctccatct
     1321 gcctcgagct gccgggcgtg ctgggcgccc ggatgatggg cggaggtcaa ggcggttcgg
     1381 ttctcgtcct gatcgaggaa gattgcttcg gcgagctcgc agatcggctg gatcgcgact
     1441 actttgcggg gttgactgag caaccgcagg tcaagctgaa ctggtgcgtg tttgcgccgg
     1501 gagctggagc gctggagccg cctcaggcct cgtagcgcgg cagcgagtgc ttttgcttgc
     1561 gcttgacgtc ccggatacct gagcgtcggt tccgataggc ggtggtcagg tggctgccgg
     1621 tcatcgggtc tagcacgagc acagtgcctt cgagccgcgt gagtttgcta tggcgctggt
     1681 ctgccgccgg gatgtcccgc cgtcccaggc agatgaagat ggccttgccg ttgcgaatgc
     1741 gtctcccgtg attcagcaca tattcgatat ctcgcgagga aagattgcgc tgcgcgaggc
     1801 gctgagaggc gtggatggaa acggattcca attgacgttc ggccatcggt gagtccttac
     1861 acactatctc ctgcggctaa ttttttgtga tgtacgtaca gtgtgtccgt atgtctttga
     1921 attgtcaagt aggtaagggt tcccagtcgg tcgaatattg gaaattcgac gaaggccacc
     1981 ccagcccgtt ggtcgccact atgaagattt gcgaaggatt cagcgcgtgc ctgcgctacc
     2041 cgccgatttg cgacatgccg cgaccgatgt tgaagtcgcc ttcggcaatg ccgtggcccc
     2101 atggcttgcg ccagattgcg gagcggatcc ggccggtcaa ttgatcgtct gttccgccgt
     2161 tccggacaat gtcgcgcaga tcgacctcat cagcgcgcag gaggcagagc cgcagcttgc
     2221 cgtccgcgct caggcggacc cgattgcagg tgctgcagaa cggttcggag atcggcgaga
     2281 tgaacccgat cgtaccggcc gcaccgggaa tcttccatac ccgcgccgga tcgccgggtg
     2341 gcgatgcgat gtgctcaagc gggccgaatt cgtactccag cctcgcctgc gtctcctctg
     2401 atgtgacgag cccttcgtcg tagaccatgc ccacgccttc catcggcatg atctcgagga
     2461 agcgcatttg ccacgggcga tcgatcgtca ggcgggcgag ctccgcaacc tcgtgctcgt
     2521 tctgaccgcg gacaacgacg gtgttgatct tgatcggcgt gatgccggcg cgctcggcgg
     2581 cgaggacccc gtcccagacc atgtcgaagc ggccgccgcg tgtcatcgcc ttgaaccgct
     2641 ccggattgag cgtatcgagg ctgacgttga tgcggtcgag cccggcttcg gccaagccct
     2701 cggccatgcg tccgagcaac agggcgttgg tggtcatcgt gacctcttcg accccgggga
     2761 agctcttgat cgcgcggacg atgtcgacta gatggggacg gatcgtcggc tcgccgccgg
     2821 tcaaccggaa cttggtgaat cccagttcag cgaaggcgcc gacgacgcga atcagctcat
     2881 catcggtcag gatctcgtcg cgcggctgga acttcatgcc catttccggc atgcaataga
     2941 cgcagcggaa gttgcagcga tcggtgagcg agatccggag atagctcatg gtccggccgt
     3001 atgcgtcctt tgcgactccc ttgccgggcg cgaagagcgt gatcggtttc gtctcggctg
     3061 tcgcgaccat gtgaactctc caatgattgg atagccaatg tttgactatg tgccgatagg
     3121 gtagcattcc gccctgatac ggcacaagac ccgcgcgttg aggagaacaa gacaacctgt
     3181 gtcccaaacc gagtacgtgc cgcagggcgc gctttcgagc tcatcgctgc gagccttgat
     3241 cctttctgac cggccgctga tccgcgacta tcgcaatctc gatacgcagc tgcagccaaa
     3301 cggtttcgac ctgacgctgg agtcagtcgc gatccatcac ggcgcgggcg tggtcgggca
     3361 atcgaacgag gatcgcgtgc tgccggatct cgtcccgctc gagttcgatt cgcggggctg
     3421 ggtcgatctc cagccgggca tctaccacat cacgtacaac gaggtcgttg cgctgccgct
     3481 caacctgatg gcgctcggtc gaccgcggtc gacgctcaac cggatcggcg tgacgatcca
     3541 caccgcggtc tgggacgccg gatacgaggg gcgttcgacg tccctgctgt cggtcatgaa
     3601 cccgaacgga gtgcagctgc agcgagacgc gcgcgtaatg cagctcgtct tcttcggcgt
     3661 cagcattccg cccgagatcg gctattcggg cgtttatcag ggggagaata tcggccgcta
     3721 gtggccgcgg cggctgtaat ccggccggcg cggcggcttg ccaggcaccg caatctggga
     3781 gcggacttgc tggacttcct tcgattgatc gcagtcgatc accgggcaga cgtagatcac
     3841 ggtgtacgtc tcctggctca tatcggtgaa ccgggtcgga cggcccatct tgccgcgcag
     3901 atgcatctca accttgtggt cggggcaaat ggggatcggc ggttcgacga cctgacggcg
     3961 aaagacgata tccagaattc gtgccaccgt tatgctcccc gagtactgct cgaccgtatc
     4021 caaaagcagc cgttatgcgt ttagtataga gagttacgga acgccggcgt aggcaggcct
     4081 gcatgaggcc ctggaagtcc acgttgatgg tagacgacat cgtaaaaatg ctggtcgagc
     4141 gatacggcga tacctggacc tccgacgagg aggcgaacct gcgcttctcg ctcgaggcgg
     4201 agcacgtgct cggcaacctc gaaggcgtcc acctgatcgg tgattgcgac ctcgaggatg
     4261 gcaggatcac gctcaacgtc tgggtcgacg agccgattta cgacctgatg ggcgccgacc
     4321 agcttgcata tgacatattc ggccggctca gtgaagagat cttctacgct gagcgccgct
     4381 tcgatgagaa gggtatccgg tatccattcg tgaccggatc gctgcgccac ggacacgttg
     4441 gccagctcct gcttcagggc ccacatgctg ccgagtttgc ggatcggcat cagttgcgta
     4501 tccggggaga cgtgcgtttc cacgcatagg gaagggtgtc ttgcacaagg taacagttga
     4561 gggaatcgag accgtcgtca ccgccggcga tgggcggaga ctcgtgcttg cgatcgagga
     4621 tgccggcatc gacatcctgc atcgctgcgg cgggaatgcc cgctgcgcaa cttgccgggt
     4681 cgaagtagtc gatggtgagc cgagcccgat cacgccggcc gaggaagagc gcctgcagcg
     4741 cgtgaccggc cgcaaaccaa acacgaggct atcgtgccag attcaggtcc gcaacgacct
     4801 gactgtccgc gtcctccgcc ggttgtccga gaacccggag ctgggcgatt ccggaccacg
     4861 gccgatcgaa tggccgtcgg accgaccctt gccaccagag gaagactagc ccttggtcct
     4921 acccggccgt ggcgtccgta cgcctgcgcc gggtatactt atgcgcggtc gtgctagatg
     4981 gggaactagc ggtgccctgt acccgcaacc cgctacagcg gggtcgaatt ccctttcgag
     5041 gttctacgga tcaggactgc ggcccaagcg caatgttgat ggtgaggtct cgttcggcgg
     5101 atctctgtga accaggtcag gaccggaagg tagcagcctt aagcagtaag gttcgggtga
     5161 acggggttgc cgacccggag taagcggtgg gtgcggcaag ctgggccgcc gtatcgacaa
     5221 agggtgcacg accaacccca cgaacatgca acgagcccgg gagtaatctc ccgggctcgt
     5281 tcatttctgg ctgatcctgg gcgattaccg gcgacgcctg cggaccggcc ggggattggt
     5341 tgcgagcgcg gcgtgtgcct gcttccggcc ggcgacattc gacagcgggc gaacatattg
     5401 atcccgctgc tttcggctct cccattcggc cagctcgacc ttgtaccgac gcttgatgtc
     5461 gaccagcagc aagacgaaga ccacgccgag cgcgagaact gtgacccaca tccagaaccg
     5521 catggcgaac gtgaacgggt tgatctggag cgcccggatc aggaagaaga agatgccggc
     5581 gccgaagatt accgacgtcc agccggccca acgccgaacc agccggaaaa tgaccgggtt
     5641 cggaaaaaga cgccggccct ggttgttgta aaccgccagc gaaaacagga agccaacgac
     5701 gaagaacacc agataaagga tcgcgagtgg accgaacact tcgttcggac cgggaacggt
     5761 cgagaggtaa tcccacgaaa ttgggttgtc caagacgttt gctccgttaa ctcactggtt
     5821 cgtgggcaaa agcatagcag tgtcgcgaat cggaattaca cccggttcag gatcagcgcc
     5881 cgcttcactt cttctattgc cttcgtgatc ttgataccac gcggacacgc ctccgtgcag
     5941 ttgaacacgg ttcggcaccg ccagacacca ccacctttgt tgaggatagc cagacgctcg
     6001 gccgctccct cgtcccggct gtcgaagata aaccggtgcg cgttgacgat cgcggcgggt
     6061 ccgacgaact cctcactggt ccagctcact gggcaggccg tcgtgcaggc ggcacagagg
     6121 atgcacttgg tggtgtcctc gaagcggtcg cgatcaactg ggctctggta tcgctcccga
     6181 ccgaccggcg catcgtcggc gatgaggtag ggcttgaccg acttgtaggc gtcgaagaaa
     6241 cgatccatgt cgactacgag gtccttgacg acatggaagc cgatgagtgg ctcgagcgtg
     6301 atcttctggc cgaattcgcc catcaggacc ttgcaggcga ggcggttgcg cccgttgatc
     6361 cgcatcgcgt ccgagccgca gatgccgtga gcgcaggagc gacggtaggt cagggtgccg
     6421 tcttcgaacc atttgatctt gttcagggca tcgagcacgc ggtcggtcgg ctccacgtcg
     6481 acccggtact cctcgaagtg cggtttgagg tcgacttccg ggttgtatcg cttgatgcgt
     6541 agggtgacct gctgcacgat gccctcctcg gcgcgcgcga cctaatactt ccgctctttc
     6601 ggctcaaacc tcgtaataga gaccggcttc ttggcatagg tcagcccgcc ggaggtcttg
     6661 cggaccatgg tgtgtgccaa ccaattctta tcgtcccgcg cctcgaagtc ggagcggtaa
     6721 tgtgcacccc ggctctcctc gcggagcagc gcgccgccga cgatcgtgtc ggcgcaatcg
     6781 agcatatatc cgagctcgag cgcctccatc aggtcggtat tgaagatcga gcccttgtcc
     6841 tggatcgcgg agtggttgta ggcatcgcga agctgcccaa gctcctgctg tgcctcggtc
     6901 aggccctcac ggttccggac gaccgagacc ttgtccgtca tcatgacctg catgcgctcg
     6961 cggatctcgg cgatggactc gcccttggca cggccaagga cgcctgagag ctcagcgttc
     7021 gtcgcgagct caggctccgc cggaagtgtc gcgagctcgg ccgtcttgat gtactcgagc
     7081 atatgccggc cggcgcggcg accgaagacg accagatcga ccagtgaatt tgtaccgagt
     7141 cgattggcgc cgtgcaccga gacgcaggct gactcgccgg ccgagtagag ccccgggatc
     7201 aatgcgccct gctcattcag caggacctgg ccttcgatat tggtcggcag gccacccatg
     7261 gcgtaatgcg ccgtaggctg aatcggcacg ccttccttct ttggctcgac gccgaggtag
     7321 gtccgcgcga aatcggtgat atccggcaac ttctcgtcga tgaccgactc gggcagatgc
     7381 gtgagatcca gcaggacata atccttgccg tcgatcccgc gcccttcctt gacttcctgg
     7441 tagatgaatc gggagaccat atcgcgcggg gcaaggtctg ccagcgtcgg cgcgtagcga
     7501 gccgcgaacg cctcgccttc gctgttccgc agaatgccac cttcgccgcg ggcagcttcc
     7561 gagagcagga tgccgagctt gtagaggccg gtcggatgga actggtagaa ctccatgtcc
     7621 atcagcggca cgccccgtcg gtaggcgatt gcgaccccgt cgcctgtgcc ggcatgcgcg
     7681 ttcgacgtga tcttgaagac ccggccgaag ccgccggtcg cgatcagcac gcacttgctg
     7741 tgcagggtgt tgacctcgcc ggtgctcaga ttccgcgtca cgacaccaca gcagcggccg
     7801 tcctccgcga aaagcagatc ggtcatgtag tgctcgtcaa agaagtggac gtcgtgcttg
     7861 atgccctgct ggtagagcgt ctggatgatc atgtggccgg tgcggtcggc ggcgtagcag
     7921 gcgcggcgga cggcgctctc gccgaagttc ttcgtgtggc cgccgaaccg gcgctggtcg
     7981 atcttgccgt cgggcgtccg gttgaagggg agtcccatgt gctcgagctc gagcaccgcg
     8041 tcgatcgcct cgcgcgcgag cacttcggcc gcgtcctggt ccaccaggta gtcgccaccc
     8101 ttgacggtgt cgaacatgtg ccattcccag tggtcttctt cggtattgcc gagggccgcg
     8161 ctgatgccgc cctgggccgc gccggtgtgc gagcgcgtcg ggtagagctt gctgacgacg
     8221 gcaacattcg ccttgcccgc catctggatt gccgccatga gtcccgcacc gccggcgccg
     8281 acaatgaccg catcgtaccg gtgatatccc atggtgctga ctcctcgcgc attggaagct
     8341 gaatcgcgca gtacgcactc ggtaacgtat ccacctgccc ggaaatcgtc aaggaacgtt
     8401 gaacaaaccg cgacatgagc ctcggctata ctttctggcg tcgagtcacc gcattgcccc
     8461 ggcaaaatcg cgccgatgcc gcgccaccct gaacggattc agcatgtcac tgtcccaaac
     8521 cgattcgccg atctggcaag atgcggcgct ggtccggcgc gcactcgagg tcggagccca
     8581 gttcgccgac aaggtcatcg agactgcccg cgtcattgcg atggtcgcgg cgccgaccaa
     8641 cgatgaacgc gatcgggccg tgctcgtggc cgacctgttc cagacacacg gcgcgtctga
     8701 cgtctcgatc gactcgatcc acgacgtcgt cggccggttg aagggcacgt cgtccgagaa
     8761 ggcgctcctg ctcgcggccc acaccgacac cgtcttctca cgcgagaccg tgctcaagat
     8821 tcaccaggac gatcagcgcg catatggtcc cgggctgggc gacaactccc tgggagtcgc
     8881 gtccgtcgtc ttgctgccgg atatgctcca ggcgcttggc gtgactcccg ccacggacat
     8941 tctgctgacc gggaatgtcg gcgaagaggg gctgggaaat ctgcgcggca tcacgcaggt
     9001 gctgaccgat cacgctgatg tcggcgcagt gatcgccgtc gagggacaaa atctcggccg
     9061 tatcacccac atcgccgtcg gcagtgttcg ccttcgcgtg accgtcaccg gtcccggggg
     9121 ccacagctgg ggcgacttcg gcaatccgag cgccatccat gcggcggcga gactgatcga
     9181 gcagctcgac cgcatcccgc tcacgcaatc cccgaagacg acgctcaacg tcggcatgat
     9241 cagcggcggg atctcgatca actcgatcgc accgaaggtg acgttcgacg tcgatctacg
     9301 ctcgaccgac gccaccgcgc tcaagcgagt cgtcgatcga gtctatgccg ccctggcttc
     9361 acgagatgaa cggattacgg ttgaacccga agttctcggc aagcggccag ccggccaggt
     9421 tgcggtcacg agccggatcg ttgtcgccgc ggccgaaatc ctgcggtcgc tttcgtattc
     9481 gccggctgcc gacgcatcct cgacggatgc caatgccgca atcgcaatgg gccttccggc
     9541 agtctgcatc ggcctgacca ctggcggcaa cgcgcaccgg ctcgatgaat acatcgacct
     9601 cgcgccggtc aagaccggat tggcccagct gatgctcctt tcgcttgcgg tctcgaatga
     9661 tctcgcccag gggcgactct cgccataaga tgcccgactt cactgacgat ctcctgtttc
     9721 ccgccgggac ctgggccgcg gttggcactg gcgacgaccg tcatggcaac acgctgcgcg
     9781 tgctcctgtt cgacggcccg attccggtcg acgatcgaac gggaccggca ccggacgtct
     9841 tcgatttcgt cgacatgcaa tgcgggatcg gactcgaatt cgcggccgtc tccagctccg
     9901 cctggctggg ccggtatgca ggcgacgacg atgcaatcga tctttcgcca tacgtcgcgc
     9961 tcgatgcgtg gtcgggaata attggcatcc agccgaggct gatcgcgccg ggagtcgagg
    10021 aggtcgccgc tcggctaggc gaagtagtcg cccgtttggg agctctcgac ccgggcgtct
    10081 tgctcaggtc cgacgcgtac ctcgggttcc gccggaatgg cgagcagcga ttgatccggg
    10141 ccgcgctggc acacagccag gccacgcacg gcgcggcatc gccgcggccc ccagagctgg
    10201 ccttcctgac gaaccgtgaa aagcccgagc ggttgaagcg ggtcaagaac tccgaccggg
    10261 tcgagccgga cgaaatcgca gcctccttcg atatcggcgg gccgatatcg aaacg
//
LOCUS       DATAOZ010000158         5226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5853874, whole genome shotgun sequence.
ACCESSION   DATAOZ010000158 DATAOZ010000000
VERSION     DATAOZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5853874"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..816
                     /locus_tag="VHR64_04345"
     CDS             91..816
                     /locus_tag="VHR64_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX3302087.1"
                     /translation="MRRFPILFCVTFSLLLVPSDVTAQATPVPKSADEVCAPFVAGTP
                     LPTPTADAGVDLAGLDFDLIFLDAMIPHVQNAIDMAIVANERSTRSEIEDFTAQELPL
                     QQAQVDAMRLWREEWYPDTPALTQSQLVHAMNMKLADSPGVGGIAGLEEMDAIHRDAD
                     LAELCSSGSEDVDLTYIDSVIAHNGSSIILARAAADRSTHREIRELSDVMISMQQYEI
                     DQLLAWREAWYQGTPIPDHHSDA"
     gene            complement(813..1649)
                     /locus_tag="VHR64_04350"
     CDS             complement(813..1649)
                     /locus_tag="VHR64_04350"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3302088.1"
                     /translation="MIESGAAQLLVSGFDSPEGPAFDRNGNLFFVNWLTSSINRLDLD
                     GNVTEFYNTGGIPAGLAFHPDGSLWVADEGDDIRGLMRITPDGHAEIVVNEYEGTPLN
                     GANDLVIDRDGVVYFSDPWRSSAENPIGGFYRYSPDGRLEQIDTGLAFPNGVALNADE
                     SAVYLAETYKTRILRYQINAEGSMGPAEEWGQTGDPPGPDGMAFDAEGNLYCAHHGGG
                     RVDIFAPDGKRIGEIPIPGAGVTNCAFGGPDRKTLVVTDVTTSSLYTVQMPIPGQQLN
                     DR"
     gene            complement(1646..3229)
                     /gene="aceB"
                     /locus_tag="VHR64_04355"
     CDS             complement(1646..3229)
                     /gene="aceB"
                     /locus_tag="VHR64_04355"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX3302089.1"
                     /translation="MTNGVSLTAEVPEHAAHVFSDEALEFVADLHREFNSRRLELLDL
                     RAQRVARIPTSFGLDFLPETKPIRDGDWRVAPASAGLIDRRVEITGPTDRKMMINALN
                     SGAKVFMADLEDANSPTWSNVIEGQINLYDAVRREIRFEQDDGKVYELEPIIATLVVR
                     PRGWHLHERHIEVDGEPASASLVDFGLTFFNNAAEQTMRGLGPYFYIPKLESHLEARL
                     WNEVFIWAQDRIGMPQGTIRATVLIETIFAAFEMDEILYELRDHAAGLNAGRWDYIFS
                     LIKTFSHSANHVLPDRAQVTMAVPFMKAYADLLVKTCHQRGAHAIGGMAAFIPTRKDP
                     EFNELAFARVREDKQREANAGFDGTWVAHPGLVAVAEAEFNAVLGDKPNQTDRQRTDI
                     EPSATTLLDTTVPGGRITEAGLRTNISVAIQYIDNWLRGIGAAAIYNLMEDAATAEIS
                     RAQIWQWIRQNVRTDDGEPITIERYRALRDEEVAKLDSPKGNRYADAVEILDTLIESE
                     EFIPFLTIPAYEYLNGVKA"
     gene            3358..4353
                     /locus_tag="VHR64_04360"
     CDS             3358..4353
                     /locus_tag="VHR64_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX3302090.1"
                     /translation="MAIAANGRVGIIANPVSGKDIRRLVAHASTFDNNEKINIVRRAL
                     LALDSLGIGEVWYLPDSYAIVERAAATAELALDLQPLPMEVIGNASDSYEAACRLVDL
                     GAGAIITLGGDGTNRVVAKGCEEVPLVAVSTGTNNVFPRMVEGTLAGLAAGLAALGVA
                     DEAIARRPRLDILINDEPRDIALIDVVTSPQQWIGARAVWEPGHIREVVLSRIMSGEI
                     GICGIGGMLFPDAAGSSFGAHIVVGGEGTSVMAPLAPGIVHEVPIAGAAKVLPGESVR
                     LQSAPGTIALDGEREIELLTDQDEIIVTLNPHGPFVVDIPGAINQGALAGVFTRS"
     gene            complement(4340..4984)
                     /locus_tag="VHR64_04365"
     CDS             complement(4340..4984)
                     /locus_tag="VHR64_04365"
                     /inference="COORDINATES: protein motif:HMM:NF039470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HEX3302091.1"
                     /translation="MQQDWLLRMIAQLSTFVMGIKRLREQKQWEEVHEQIGDGIGRFV
                     GLNASLVHAISEDDLIQLLSTRGGVDLDRWWALAELLREEGLALEEQGDTAGAERAFA
                     KSLRLYLEVLHEAEEVPTYLNVTDLEAVITRMLDRDLSRSTRLALIEYLVDTGRYDQA
                     ENVALWALDGPDARALDDARAFYESLRAKSDAELEAGGLSREEIALGLDALTTE"
BASE COUNT          889 a         1600 c         1705 g         1032 t
ORIGIN      
        1 aatgcaccgg ccgttcaacg gtcctggcaa ccgtaaaggt tcggtccgga gacacacggg
       61 cagatcgcca tctgccccta caattgcccg atgagacgat tccccatcct tttctgtgta
      121 acgttctcgc ttttgctggt gccctccgac gttacggcgc aggcgacgcc agttccaaag
      181 tcggcggacg aggtttgcgc gccgtttgtg gccggcacgc cgttgccgac gccgacggca
      241 gatgccgggg ttgatctggc ggggcttgat ttcgatctga tctttctcga tgcgatgatc
      301 ccccacgtcc agaatgcgat tgacatggcg atcgttgcca atgagcgcag cacgcggagc
      361 gagatcgagg acttcaccgc ccaggagttg ccgcttcagc aggcgcaggt cgatgcgatg
      421 cgactgtggc gcgaagagtg gtatcccgac acgccggcgt taactcaatc ccagctcgtg
      481 catgcgatga acatgaaact cgccgacagc ccgggagtcg gtggcattgc cggcctcgag
      541 gagatggatg cgattcaccg agatgccgat ttagccgagc tctgctcctc cggcagcgaa
      601 gacgtcgatc tcacgtacat cgattcggtc attgcccaca atggcagcag catcatcctg
      661 gcgcgtgctg ccgcggaccg ttcgacgcac cgggagatca gggagctgtc cgacgtgatg
      721 atctcgatgc agcaatacga gatcgaccaa ctcctcgcct ggcgtgaggc gtggtaccag
      781 ggcacgccga tcccggacca tcactccgac gcctagcgat cgtttaactg ttgacccgga
      841 attggcattt ggactgtgta gagcgaggag gtcgtgacgt cagtcacgac gagcgtcttc
      901 cgatctgggc cgccgaatgc gcagttggtc acgccggcgc cggggatcgg gatctcgccg
      961 attcgctttc cgtccggcgc gaagatgtcg actctcccgc cgccatggtg agcgcagtag
     1021 aggttcccct cagcatcgaa ggccatgccg tcgggacccg gcggatcgcc ggtctgtccc
     1081 cattcctcgg ctggacccat cgagccttcc gcgttgatct gatagcggag gatgcgcgtc
     1141 ttgtaggtct cggcgaggta gacggccgat tcgtccgcat tcagtgcgac tccattcggg
     1201 aaggcaaggc cggtgtcgat ctgctcgagg cgaccgtctg gagagtagcg gtagaagccg
     1261 ccgatcggat tctcggcgct cgatcgccac gggtcggaga agtagacgac gccgtcccgg
     1321 tcaatgacga ggtcgttcgc cccgttaagc ggcgtgccct cgtattcgtt gaccacgatc
     1381 tctgcgtgtc cgtccggcgt gatccgcatc aggccgcgga tgtcgtcacc ctcgtcagca
     1441 acccaaagcg agccgtcggg atgaaaggcg agtccggccg ggataccgcc ggtgttgtag
     1501 aactcagtga cgttgccgtc caggtcgagg cgattgatcg agctggtcag ccagttgacg
     1561 aaaaacaggt tgccgttccg gtcgaacgcc ggtccctccg gggaatcgaa cccggagacc
     1621 agcagctgcg cggcgccgga ctcgatcatg ccttgacccc gttgaggtac tcgtacgccg
     1681 ggatcgtcag gaatgggatg aactcctcgc tctcgatcag cgtgtcgaga atctcgaccg
     1741 cgtcggcata gcgattgccc ttcggcgagt cgagcttggc gacctcctcg tcccggaggg
     1801 cgcgataccg ttcgatcgtg atgggctcgc cgtcgtcggt gcggacattc tggcggatcc
     1861 actgccaaat ctgggcgcga gagatctcgg cggtggcagc gtcttccatc aggttgtaga
     1921 tcgcggccgc gccaatgcca cggagccagt tgtcgatgta ctggatcgcg acgctgatat
     1981 tcgtgcgcag tccggcctcg gtgatcctgc cgcccggaac tgtggtgtcg agcagggtcg
     2041 tggccgaagg ttcaatgtcc gtgcgctgcc ggtcggtttg gttcggtttg tctccgagca
     2101 ccgcgttgaa ctcagcttcc gcgacggcaa cgaggccggg atgcgccacc catgtgccat
     2161 cgaagcccgc attggcctcg cgttgcttgt cctcccgaac ccgggcaaac gcgagctcgt
     2221 tgaactcggg atccttgcgg gtcgggatga acgccgccat gccgccgatc gcatgcgcgc
     2281 cgcgctggtg gcaggttttg accagcagat cggcatacgc cttcatgaac gggacggcca
     2341 tcgtgacctg ggcgcggtcg ggcaggacat ggttggcgct gtggctgaat gtcttgatca
     2401 ggctgaagat gtaatcccag cgcccggcat tcagccctgc ggcgtgatcc cgcagctcat
     2461 agaggatctc gtccatctcg aacgcggcga aaatcgtctc gatcagcacc gtcgcgcgga
     2521 tcgtgccctg tggcatgccg atccggtcct gcgcccagat gaagacctcg ttccagaggc
     2581 gggcttcgag gtgactttcc agcttcggga tgtagaaata cgggccgagt ccgcgcatcg
     2641 tttgttcggc cgcattgttg aagaaggtca ggccgaaatc gaccagcgat gccgacgccg
     2701 gctcgccgtc gacctcgatg tgacgctcgt gcaggtgcca gccgcgcggc cggacgacga
     2761 gggtcgctat gatcggctcg agctcataca ccttgccgtc atcttgctcg aagcggattt
     2821 cgcggcgcac cgcgtcgtag aggttgatct gaccctcgat gacgttcgac caggtgggag
     2881 agttggcgtc ctcgaggtcg gccatgaaga cctttgcgcc cgagttgagc gcgttgatca
     2941 tcattttccg gtcggttggg ccggtgatct cgacgcggcg atcgatcagc ccggctgatg
     3001 ccggagcgac ccgccagtcg ccatcgcgga tcggtttggt ttcgggcagg aaatcgagcc
     3061 cgaagctggt cgggatccgg gcgacgcgct gggcccggag atcaagcagc tcgagccggc
     3121 gcgaattgaa ctcgcgatgc aggtcggcga cgaattcgag agcctcgtcc gaaaagacgt
     3181 gcgccgcgtg ctccggcact tcggccgtca ggctaactcc gttggtcacg tttacctccc
     3241 tgttctccgg tcgttgcaac ctgaccatat tcgcatgttt ggcattggtc gtgccctgaa
     3301 tcggtacact cccgccagtt gttgaccgtg gctggcgtgt tcgaggaggg gaaacggatg
     3361 gcgattgctg caaacgggag agtggggatc atcgcgaacc ccgtctcggg caaggacatc
     3421 cgccggctgg ttgcgcacgc ctcgaccttc gacaacaacg agaagatcaa catcgtccgg
     3481 cgcgccttgt tggcgctcga ttcgctggga atcggcgaag tctggtacct gcccgactcg
     3541 tacgcgatcg ttgagcgcgc ggccgcaacc gccgaactcg ccctggacct gcaaccgttg
     3601 ccgatggaag tgatcggcaa cgcgagcgat tcgtatgagg ccgcctgccg cctggtcgac
     3661 cttggtgcgg gcgcgatcat cacgctcggc ggcgatggca cgaatcgggt cgtggccaag
     3721 ggctgcgaag aggtgccgtt ggttgccgtt tcgaccggaa ccaacaatgt gttcccgcgc
     3781 atggtcgagg ggacgctggc cgggctggcg gccggtcttg ccgcgctggg tgtggcagat
     3841 gaggcgatcg cccggcgacc gcggctcgac atcctcatca acgacgagcc gcgggatatc
     3901 gccctgatcg acgtggtgac ctcgccgcag cagtggatcg gcgcgcgggc agtctgggag
     3961 cccggccaca tccgcgaggt cgtgctgtcg cggatcatga gcggcgagat cggcatctgc
     4021 ggaatcgggg ggatgctgtt cccggatgcg gccggatcca gcttcggcgc gcatatcgtc
     4081 gtcggcgggg aggggacgag cgtcatggcg ccgctcgcgc ccggcatcgt gcatgaggtg
     4141 ccgatcgccg gcgcggccaa ggtgttgccg ggcgaatcgg tcaggttgca gtcggcgccg
     4201 gggacgattg cgctcgacgg cgaacgggag atcgagctgc tgaccgatca ggacgagatc
     4261 atcgtcacgc tcaatccgca tggcccgttc gtggtcgata ttccgggcgc gatcaaccag
     4321 ggcgcgctgg cgggagtctt cactcggtcg tgagcgcgtc cagaccgagc gcgatctcct
     4381 cgcgactcag tccgccggcc tccagctcgg catccgattt cgcgcggagc gactcgtaga
     4441 aggcgcgggc atcgtccaat gctcgcgcat caggtccatc gagggcccaa agtgcgacgt
     4501 tctctgcctg gtcgtatcgc ccggtatcga cgaggtactc gatcagggcc agccgggtgg
     4561 agcgtgagag gtcacggtct agcatgcgcg tgatcactgc ttccaggtcg gtcacgttca
     4621 ggtaggtcgg cacctcctcg gcctcgtgca ggacttcgag gtagaggcgg agcgacttcg
     4681 cgaaggcacg ctcagccccg gccgtgtctc cttgctcctc gagcgccagg ccttcctcgc
     4741 gcaggagctc ggccagcgcc caccaacgat cgagatcgac cccgccacgc gtgctgagga
     4801 gctggatcag gtcgtcctcg gagatcgcgt ggacgagcga ggcgttgagc ccgacgaacc
     4861 ggccgattcc gtcgccgatt tgctcgtgga cttcttccca ctgcttctgc tcgcgcaacc
     4921 gctttatgcc catcacaaac gtgctgagct gcgcaatcat ccgaagcaac caatcttgtt
     4981 gcacgtgagc ctccacggtt cttggaccac aggactttca gttggtacct cattgaagtc
     5041 tgtttgtgat accgtcgttc acaaataatc acgacgtccc ggattaccac gagattgaag
     5101 atcaagcgct ctttgtgccc gggttcgctg cgttgacact tgaggccgcg acatgaaggt
     5161 gtcttgtctt gtcctggcag tcttggctgc aattactgtt cttgcaacct cttcgttgcc
     5221 gtacgc
//
LOCUS       DATAOZ010000159         6276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7431809, whole genome shotgun sequence.
ACCESSION   DATAOZ010000159 DATAOZ010000000
VERSION     DATAOZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7431809"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1101)
                     /gene="pheS"
                     /locus_tag="VHR64_04370"
     CDS             complement(19..1101)
                     /gene="pheS"
                     /locus_tag="VHR64_04370"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX3302092.1"
                     /translation="MATSPPVLDQLSSIESTALDELSSVSDLGSLAEWERSYLGSTGA
                     ITGVLRGMGQIPAELRPEVGRTANQVKQRLTSAFDERKAALQASALTDKLATEAIDIT
                     LPGRTPLVGTVHPISQMIDEVSEIFAYLGFETVFGPEVEIGKYNFDMLNIPANHPARD
                     VWDTIIVNDERDEVVLRTHTSPMQARVMEQRTAPLRVIVPGRVYRYEQQDASHEWMFH
                     QIEGLALDDRITMADLKGTLTELARQLFGDDTRVRFRCDFFPFVEPGVEFAVSCPFCG
                     GKGCRVCKQSGWIEMGGAGMVHPNVLRWVNYDPDQVQGWAFGLGVERLVMVKYGVNDI
                     RAFAGNDLRFLRQFARADIGLGVVRA"
     gene            1457..2746
                     /gene="eno"
                     /locus_tag="VHR64_04375"
     CDS             1457..2746
                     /gene="eno"
                     /locus_tag="VHR64_04375"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX3302093.1"
                     /translation="MSATITSIRAREILDSRGNPTVEVDVELASGSSGRAAVPSGAST
                     GAYEAIELRDGDKRRYGGKGVLTAVGNVNDEIAQNVVGMNALDQRDIDSLMIEIDGTP
                     NKGRLGANAILGVSLAVARAAATWSGLPLYRYLGGPNACTLPVPMMNILNGGKHALGS
                     SVDMQEFMVMPVGAASFREGLRMGTEIFHALRGILHDRGLGTGVGDEGGFAPSLESNE
                     AAIKVVTEAISKAGYRPGEDLVIALDPASTELYHGKKYQLKGEGRTLSSEDMVDYWAD
                     WSKKYPIRSIEDGLSEDDWDGWKALTERVGSKVQLVGDDLFVTNTERLSRGIREHAAN
                     SILVKLNQIGTLTETFDTIEMAHRAGFTSVTSHRSGETDDTFVADLAVATNSGQIKTG
                     APSRMDRVAKYNQLLRIEEMLGNAAVYPGEQIFSRFG"
     gene            complement(2755..3174)
                     /locus_tag="VHR64_04380"
     CDS             complement(2755..3174)
                     /locus_tag="VHR64_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX3302094.1"
                     /translation="MSLLLDTNVLSEWQKTQAAEAVVRFGEEIKHQNTMISVISVAEF
                     GYGVARLPLGRKRTELELWLANVENEYSGRILAIELETARVWANLVARLRGSGRNITE
                     ADAWIAATAIQHGLQLATRNVRDFAETGVPLINPWDF"
     gene            complement(3171..3413)
                     /locus_tag="VHR64_04385"
     CDS             complement(3171..3413)
                     /locus_tag="VHR64_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX3302095.1"
                     /translation="MDWKLAEAKNKFSEVYRLATTEGPQRITKQGAEPVIVITEAEYE
                     TLKRRKPTFVEYLMSGPRIDDLELPSRNWNMRDVDL"
     gene            complement(3498..5048)
                     /gene="gpmI"
                     /locus_tag="VHR64_04390"
     CDS             complement(3498..5048)
                     /gene="gpmI"
                     /locus_tag="VHR64_04390"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX3302096.1"
                     /translation="MTTNTTYRPVALVILDGWGIGKSGPGNAIEAAATPTMERILATC
                     PHTTLFTSGAAVGLPEGQMGNSEVGHLNIGAGFIVHQWISRIDNAIDEGRLAANPILT
                     SAIDHCVKTDRTLHIGGLVSDGGVHSHIRHIVALIEIAAARGLKKIAIDAFTDGRDTL
                     PTSGIGFIVDLEQKLREVGVGRIATVSGRYYAMDRDKRWQRTQIAYDAIVSGQGRAAS
                     SAKQAIQTSYDAGVTDEFIIPTVIPNPDGPDGMQDGDVFLFANFRSDRGRQLTHALSD
                     PDFTGFERSRVVATTCRVITMTRYEEGLPVDVLFEPHDIVTPLAKVISDAGLAQFHCA
                     ETEKYPHVTFFINGGREEPFPGEVRKMIPSPRVATYDLQPEMSAIPVADATVEAIQSG
                     KYDFIVVNFANPDMVGHTGNIPAVIKAVETVDAQLARVLAALQKVGGVALVIADHGNA
                     EEEIDAATGGPMTAHTTNPVPCVLVGDGQVTLRNGAVLSSVAPTVLHLLGLPIPADMT
                     TPSLLGSE"
     gene            5157..6242
                     /locus_tag="VHR64_04395"
     CDS             5157..6242
                     /locus_tag="VHR64_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX3302097.1"
                     /translation="MAVYDVTMIPGDGIGPEVAEATRRVLEATGVQFNWDRRDIGMAA
                     LEKHGDLIPQETLDSIRKNKIGIKGPTTTPIGEGFRSVNVGLRQELGLYANLRPGKTI
                     KGVQSTFSDIDIVVVRENMQGMYSGVEFDTGKPETAEVIAKINELNKKKVDPSAAISI
                     KIITEEATRKIATFAYEYARANKRKKVTIVHKANIMKFSDGLFLRVAQEVAANYPDIE
                     TNDRIIDNMCMQLMQKPDQYDVLVMANLYGDIVSDLVAGMVGGLGVAPGGNIGEGVAV
                     FEPIHGSAPSHAGKNEANPTAMILSGAMMLRHLGELEAAIKVEQAVEQVIGEGTKVTY
                     DLREDRDPSKAVGTSQMAEAIIAAIQA"
BASE COUNT         1211 a         1904 c         1969 g         1192 t
ORIGIN      
        1 gccacttcat cggaaccttc atgcgcggac cactcccagg ccgatatcag cgcgcgcaaa
       61 ttggcgcagg aagcgcaggt cgttgcctgc gaaggcgcgg atatcattaa caccgtactt
      121 gaccatgacc agccgctcga ctccgaggcc gaacgcccaa ccctgcacct ggtccgggtc
      181 atagttgacc cagcgcagaa cgttcgggtg gaccatgcct gccccgccca tctcgatcca
      241 accggactgc ttgcagaccc gacaaccctt gccgccgcaa aaggggcagg agaccgcgaa
      301 ttcgacgccc ggctcgacga acgggaagaa gtcgcagcga aaccggaccc gagtgtcatc
      361 gccgaagagc tggcgcgcca actccgttag cgtgcccttg agatcggcca tcgtgattcg
      421 gtcgtcgagg gcgagaccct cgatctggtg gaacatccac tcgtgcgagg cgtcctgctg
      481 ctcgtaccgg tagacgcggc ccggcacgat tacccggagc ggagcggtac gctgctccat
      541 cacccgggcc tgcatcggag atgtgtgcgt gcgcaggacg acctcatcgc gctcgtcgtt
      601 gacgatgatc gtgtcccaga catcccgggc cgggtgattg gccgggatat tgagcatgtc
      661 gaagttgtac ttgccgatct ctacttcagg accgaatacg gtctcgaagc cgaggtaggc
      721 gaagatctcg ctcacttcgt cgatcatctg cgagatcggg tgaacggtgc cgaccagcgg
      781 tgtccggccg gggagcgtga tgtcgatcgc ctcggtcgcc agcttgtcgg tcagggcgct
      841 cgcctggagg gcggccttgc gctcgtcgaa cgcgctggtg agtcgttgct taacctggtt
      901 tgcggttcgg ccgacctcgg gccggagctc ggccgggatc tgacccatac cgcgcaacac
      961 tccggtgatc gcgccggtcg atccgaggta cgagcgttcc cattcggcca gcgagcccag
     1021 atccgagact gacgagagct cgtcgagcgc cgtggattct atggagctga gttggtcaag
     1081 gactggtggt gaagttgcca ttttgaatgt ccgcttgatt tccgcgcata caaaaacccc
     1141 tcggcaggga cgaggggttg ccacgcggta ccaccctgct tcaaccggac acgagacccg
     1201 gttgcgcttg atgcctgata tcggaggctc cggcccgact acgcaacacc agcgtcttca
     1261 tcgggcagct cgggaatgaa ttcgccggtg cggttccggt tccgctcgca gccgtttagc
     1321 ggaactctct ggacggtcgc tgccgggtac tggtttccct catcgcgata ttcagatgtg
     1381 cgcaaagtat aaccggtcaa cctcctatac tcaggccggt attcaggcag attcgatcga
     1441 tgacagggag aagcgcgtgt cggcaaccat cactagcatc cgggcgaggg agattctgga
     1501 ttcgcgcggg aatcccaccg tcgaggtcga tgtcgagctt gccagcggtt catcaggccg
     1561 ggcggccgtc ccctccggcg cgtcgacggg cgcctacgag gcgatcgagc tgcgcgatgg
     1621 cgacaagcgc cggtacggcg gcaagggtgt tctgacggcc gtcggcaacg tcaatgacga
     1681 gattgcgcaa aatgtcgtcg gaatgaacgc gctcgatcag cgcgacatcg attcgctgat
     1741 gatcgagatc gacggcacgc cgaacaaggg tcgactcggc gccaatgcca tcctcggtgt
     1801 ttcgctggct gtcgcccggg ccgctgccac ctggtcgggt ttgccgcttt atcggtatct
     1861 cggcggaccg aatgcctgca ctctgcccgt cccgatgatg aacatcctca acggcggcaa
     1921 gcacgcgctc ggttcgagcg tcgacatgca ggaattcatg gtcatgcccg tcggcgcagc
     1981 ctcgttccgc gagggactgc ggatgggcac cgagattttt cacgcactgc gtggcatcct
     2041 gcatgaccgc ggactgggca ccggcgtggg tgacgagggc gggtttgcgc cgtcgctgga
     2101 gtcgaacgag gcggcgatca aggtcgtgac cgaggcgatc tcgaaggcgg gataccggcc
     2161 cggcgaggac ctcgtgatcg cgctcgaccc cgcctcgacc gagctctatc acggcaagaa
     2221 ataccagctc aagggcgagg gccgaacgct ctccagcgag gatatggtcg attactgggc
     2281 cgattggtcg aagaagtatc cgatccgctc catcgaggat gggttgtccg aggatgactg
     2341 ggatggctgg aaggcgctga ccgagcgggt cggatcgaag gtccagctcg tcggcgacga
     2401 cctcttcgtg accaacacgg agcgactcag tcgggggatc cgggagcacg cggccaactc
     2461 gatcctcgtc aagctcaacc agatcgggac cctaaccgag acgttcgaca cgatcgagat
     2521 ggcgcaccgg gccggattca cgtcggtgac atcgcaccgg tcgggggaaa cggacgacac
     2581 gttcgtggcg gacctggccg tggcgaccaa ttccggacag atcaagaccg gcgcgccgtc
     2641 ccgcatggat cgcgtcgcca agtacaacca gctgctgcgc atcgaagaga tgctcggcaa
     2701 cgccgccgtc taccccggcg agcagatctt cagtcgattc gggtagcgat tggtctagaa
     2761 gtcccacgga ttaatgagcg gaacaccggt ttctgcaaaa tcccgaacgt ttcgagtcgc
     2821 aagctgaagg ccatgctgga tcgcagttgc ggcgatccac gcgtcggcct cggttatgtt
     2881 ccgcccagaa ccgcgaaggc gggcaacaag gttggcccag actctcgccg tttctaattc
     2941 gatggcgagt attcgtccgg agtattcgtt ctcgacgttt gcgagccaca actctaactc
     3001 ggttcgcttg cgaccaagcg gcagccgcgc aactccgtaa ccaaactcag cgacggaaat
     3061 aacgctaatc attgtgttct ggtgcttgat ctcctcgccg aatctgacta ctgcctcggc
     3121 agcctgagtt ttttgccact ctgagagcac gttcgtgtcc agaaggaggc tcacaggtcg
     3181 acgtctctca tgttccagtt cctggaggga agctcaagat cgtcgatcct ggggccgctc
     3241 atgagatatt cgacgaacgt cggctttcgc cgcttgagcg tctcgtattc agcttcggtg
     3301 atcacgatta ccggctcggc gccctgtttc gtgattcgct gtggcccttc ggtcgttgcc
     3361 aggcggtaaa cctcgctgaa tttgttcttc gcttcggcca gtttccagtc catgccatac
     3421 gcccccttgc ttggctagat tgtctagatc aatcttatca ggtagatcaa tcgcttcccg
     3481 gcccgggaga cggattgcta ctcggagcca aggagcgaag gcgtagtcat atctgcgggg
     3541 atgggcaggc cgaggaggtg caggaccgtc ggcgcgaccg atgagaggac ggctccgttc
     3601 cggagcgtga cctggccgtc cccgaccagg acgcagggca ccgggttcgt ggtgtgggcg
     3661 gtcattgggc cgccggtggc tgcgtcgatt tcctcctcgg catttccgtg atccgcgatg
     3721 accaacgcaa cgccgccaac cttctggagg gccgcgagaa ccctggccaa ttgcgcatcg
     3781 acggtctcga ccgccttgat cacggccgga atgtttccgg tgtggccgac catgtccggg
     3841 ttcgcaaagt tgacgacgat gaagtcgtat ttgccggact ggattgcctc gacagtggca
     3901 tcggcgaccg gaatcgcgct catctcgggt tgcaggtcgt aggtcgcgac cctgggcgag
     3961 ggaatcatct tgcggacttc gcccgggaag ggctcttccc gccctccgtt gatgaaaaac
     4021 gtcacgtggg gatacttctc ggtttcggcg caatggaact gggcgagccc cgcatccgag
     4081 atcacctttg ctagcggtgt gacgatgtcg tgcggctcga atagcacgtc gaccggcagc
     4141 ccctcctcat accgcgtcat cgtgatgacg cgacacgtcg ttgccactac cctggatcgc
     4201 tcgaatcctg tgaagtccgg atcggacaat gcgtgcgtca gttgccggcc acgatcggag
     4261 cgaaagttgg cgaacaggaa gacgtcacca tcctgcatgc catccggtcc atctggattc
     4321 gggatcacgg tcgggataat gaactcgtcg gttacgccgg catcatatga cgtctgaatc
     4381 gcttgcttgg cactcgaggc cgctcgaccc tgcccggaga caattgcgtc gtacgcgatc
     4441 tgcgtgcgct gccagcgctt gtcccggtcc atcgcgtagt aacgaccgct gaccgttgcg
     4501 atccggccaa ctcccacctc acgcagcttc tgctcgagat cgacgatgaa cccgattccg
     4561 ctggtcggga gggtgtcgcg cccatccgtg aatgcgtcga tcgcgatctt cttgagtcct
     4621 cgcgcggcgg cgatctcgat cagcgctacg atgtggcgga tgtggctgtg cacgcctccg
     4681 tcgctgacca ggccgccgat atgcagcgtt cgatctgtct tgacgcagtg atcgatcgcg
     4741 gaggtcagga tcggattcgc cgcaagcctg ccttcgtcaa tggcattgtc gatccggctg
     4801 atccactggt ggacgatgaa gcccgccccg atattgaggt gaccgacctc agagttgccc
     4861 atctgtccct cgggcaatcc gaccgcagca ccggacgtaa agagcgtcgt atgcgggcac
     4921 gtggccagga tgcgctccat tgttggggtt gcggcagctt caatcgcatt gcccgggccg
     4981 ctcttgccga taccccagcc atcgaggatc acgagggcta ccggtcgata ggtcgtattc
     5041 gtggtcatga gttggcaatg tctcctctgc ctgtcgcgcc gcccgcaccg tcaagggtat
     5101 tatccagagc ggtattcgac ccaccgacac ccaacacaag gagttgtgcg ttcaaaatgg
     5161 ccgtgtacga cgtgacgatg atcccgggcg atggtattgg gccagaggtt gccgaggcga
     5221 cccgccgcgt gctcgaagcg accggggtgc aattcaactg ggatcgccgc gacatcggca
     5281 tggcggcgct ggagaagcac ggcgacctga tcccgcagga gacgctcgat tcgatccgca
     5341 agaacaagat cggcatcaag ggaccaacca cgacgccgat cggcgagggc ttccgcagtg
     5401 tcaatgtcgg cctccggcag gagctcggac tctacgccaa cttgcgaccg ggcaagacga
     5461 tcaagggtgt gcagagcacc ttctccgata tcgacatagt ggtcgttcgt gagaacatgc
     5521 agggcatgta ctcgggcgtc gagttcgata ccggcaagcc cgagacggcc gaggtcatcg
     5581 ccaaaatcaa cgagctgaac aagaagaagg tcgatccttc ggcggcgatc agcatcaaga
     5641 tcatcaccga agaagcaacg cgcaaaattg cgaccttcgc ctacgagtac gcccgtgcca
     5701 acaagcgcaa gaaggtcacg atcgtccaca aggcgaacat catgaagttc agcgacggcc
     5761 tcttcctgcg ggtggcgcag gaggtcgcgg cgaactaccc ggatatcgaa accaacgacc
     5821 gcattatcga caacatgtgt atgcagctca tgcagaagcc ggaccagtac gacgtgctgg
     5881 tgatggccaa tctctatggc gacatcgttt cggacctggt tgcaggcatg gtcggtggcc
     5941 tcggcgtcgc gccgggcggc aacatcggcg agggcgttgc cgtcttcgag ccgatccatg
     6001 gctccgcgcc gagccacgcg ggcaaaaacg aggccaaccc gacggcgatg atcctctcag
     6061 gggcgatgat gctgcggcac ctcggtgagc tggaggcggc gatcaaggtc gagcaggcgg
     6121 tcgagcaggt catcggcgaa ggcaccaagg tgacctacga cctccgcgaa gaccgcgacc
     6181 cgagcaaggc ggtcgggacc tcgcaaatgg ctgaggctat catcgcggct attcaggcat
     6241 aaactcacac gcaaatggaa acggggggcc tgtcgc
//
LOCUS       DATAOZ010000160         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6114858, whole genome shotgun sequence.
ACCESSION   DATAOZ010000160 DATAOZ010000000
VERSION     DATAOZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6114858"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1593)
                     /locus_tag="VHR64_04400"
     CDS             complement(295..1593)
                     /locus_tag="VHR64_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302098.1"
                     /translation="MPLPDKPSDAPEHDAYAALRFRDFRFLISASFLISFSQAVLSVI
                     VGYELYLRTGSALALGLVGLVQIVPNVILSMPAGQYVDQHDHRLIAAAAAGLIALSAA
                     FLALLTHYEGPLVLIYACLFLIGLGRVFNMATPAPLMASIVPPDRFGNAAAWRSSSNQ
                     TASILGPAAGGFGVAVLGSAASVFAIAAVLLAIASALLSLIKARPSPREREKMSKDSI
                     LAGIRFIRSTEVLLATITLDMVAVMLGGATALLPIFAEDVLHVGAGGLGLMRAAPAAG
                     AVLASVAIAHKGPFKAAGPTILVAVAGFGLATILFGVSRAMPLSLMALALIGAFDAIS
                     MVIRSTLQLTLTPDHMRGRVGSVHYMFVGMSNEFGEFESGLVATLIGATAAVVLGGIG
                     TLLVVPAIAIAWPEVRRLRQIEPRESEITAKVPAAASAGK"
     gene            1710..>2594
                     /gene="melA"
                     /locus_tag="VHR64_04405"
     CDS             1710..>2594
                     /gene="melA"
                     /locus_tag="VHR64_04405"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF011657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEX3302099.1"
                     /translation="MPKITFIGAGSTVFARNLLGDILAYPELHDSTITLFDIDADRLS
                     TSEIVARKVAEARGAHPTIESTTDRRRALDGADYAICMIQVGGYKPATVTDFEIPKKY
                     GLRQTIADTLGIGGIMRGLRTIPVLLEMCRDMEALCPDVTFLQYVNPMAMNCWAISRA
                     SSIKTVGLCHSVQGTAKQLARNIGVPVEEIEYVCAGINHMAFYLKFERDGVDLYPEIR
                     RVLAEGRAPMDNLVRYEMLKRLGYFVTESSEHFAEYVPWFIKRDRPDLIDNFNIPIDE
                     YPRRCEKQIKEWNALRTEY"
BASE COUNT          525 a          838 c          819 g          412 t
ORIGIN      
        1 cgaccacatc cagttgatcc agcactatgt cgatctcggc ttcaatgagg tctacgtcca
       61 caacgtgggc cgcaaccagg ccgagttcat cgacacctac ggcaagcacg tcatcccgaa
      121 cgtcaagttc ccggggtaag cacccagcgg tagtggcggg gctccgtacc cgcctggagc
      181 tgcccgtagc aacgttcgca agcacgcacc cgcgtattga gttcacgccg ggacggtggt
      241 cgatgcattg cgagattccg ttcggcgaat cgaggcgggt acggagccct gccactactt
      301 accggcagaa gcggcggccg ggacttttgc tgtgatctct gattctcggg gctcgatttg
      361 gcggagacgg cggacctctg gccaggcaat tgcgattgcc gggacgacca gcagcgtgcc
      421 gattccgccg agtacgactg ctgctgtcgc gccgatcagc gtcgcaacga gacccgactc
      481 gaactcgccg aactcgttcg acataccgac gaacatgtag tggaccgagc cgacccggcc
      541 gcgcatgtgg tccggcgtca gggtgagctg gagcgtgctc cggatcacca tactgatcgc
      601 atcaaacgcg ccgatcaagg cgagcgccat cagcgagagc ggcattgccc gcgagacgcc
      661 gaagaggatc gtcgccagtc caaatccggc gaccgcgacc aggatcgtcg gtcccgctgc
      721 cttgaacggt cccttgtgcg cgatcgcgac cgacgccagc accgcgccgg cggctggagc
      781 cgcgcgcatc aggcccaggc cacccgcgcc gacatgcagc acgtcctcgg cgaagatcgg
      841 cagcagggca gtcgcgccgc cgagcatgac cgcgaccatg tccaatgtga tcgtggccag
      901 caagacctcg gtgctgcgga tgaagcggat acccgccagg atcgagtcct tcgacatctt
      961 ctcccgttcg cgcggacttg gtcgggcctt gatcaggctg agcagggctg atgcgatcgc
     1021 aaggagcacg gccgcgatcg cgaataccga cgccgcgctg cccagcacag caacgccgaa
     1081 gccacccgcc gcgggaccga ggatcgaggc ggtctgattc gagctcgagc gccaggctgc
     1141 ggcattgccg aagcggtccg gcggcacgat cgaagccatc agcggcgccg gggtcgccat
     1201 gttgaagacc cgcccgagcc cgatcaggaa caggcaggca tagatcaata ccagcggacc
     1261 ttcgtagtgc gtcagaagcg ccaggaatgc tgccgatagc gcgatcagac cggcggcggc
     1321 cgcggcgatc agacggtgat catgctggtc gacgtactga ccggccggca ttgagaggat
     1381 gacgttcggc acgatctgga ccagcccgac cagaccgagc gcgagggcgg atccggtccg
     1441 caggtacagc tcgtagccga cgatcacgct caggaccgcc tgcgagaagg agatcaggaa
     1501 gctggccgaa atcaggaacc ggaagtcccg gaatcgcagc gccgcgtagg catcgtgttc
     1561 cggtgcgtcg gacggcttgt caggcaacgg cattgggaga atccagtgag caattgagca
     1621 atacgcgtca gcatacaact ccgcgccgtc gcgtcccgcc ggatatgctt ccggcacgga
     1681 gttcgattac cagggagccc acacctcaga tgccgaaaat caccttcatc ggcgccggca
     1741 gcacggtctt tgcccgcaac ctgctcggtg acatcctcgc ctaccccgaa ctgcacgatt
     1801 cgacgatcac gctgttcgac atcgatgccg accggctttc gacgtcggag atcgtcgctc
     1861 gcaaggttgc ggaggcacga ggcgcgcacc cgacgatcga gtcgacgacc gaccgtcgcc
     1921 gcgctctcga tggcgccgat tacgcgatct gcatgatcca ggtcggcgga tacaagccgg
     1981 ccactgtgac cgatttcgag atcccgaaga agtacggcct gcgccagacc atcgccgaca
     2041 cgctcggcat cggcgggatc atgcgtggcc tgcggacgat tccggtgtta ctcgagatgt
     2101 gccgggacat ggaagcgctc tgcccggatg tgacattcct gcagtatgtc aacccgatgg
     2161 cgatgaactg ctgggccatc agccgcgcct cgtcgatcaa gacggtgggt ctgtgccaca
     2221 gtgtgcaggg aacggcgaaa cagctcgccc ggaatatcgg tgtgccggtc gaggagatcg
     2281 aatacgtctg cgccggcata aaccacatgg ccttctacct gaagttcgag cgcgacggcg
     2341 tcgacctcta cccggagatc cggcgtgtcc tggccgaagg tcgagcgccg atggacaacc
     2401 tggtccgcta cgagatgctc aagcggctcg gctacttcgt caccgagagc agcgagcact
     2461 tcgcggagta cgtgccctgg ttcatcaagc gcgaccgccc ggacctgatc gacaacttca
     2521 acatcccgat cgatgaatac ccgcgccgct gcgaaaagca gatcaaggaa tggaatgcgc
     2581 tccgcaccga atac
//
LOCUS       DATAOZ010000161         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1910839, whole genome shotgun sequence.
ACCESSION   DATAOZ010000161 DATAOZ010000000
VERSION     DATAOZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910839"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VHR64_04410"
     CDS             <1..1078
                     /locus_tag="VHR64_04410"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3302100.1"
                     /translation="SVGRLDAAVELVDRFITEGVPGAGLVVAEHGEIVSEYYAGMARL
                     DRPADAETLWPLASISKLYAAAGCMAAIEEGRIALSTKAAQIFPEFTQGGRGKITLRH
                     LLTHTSGLPYESDQMADRLAAKISLEEMVRESYEGDLLFTPGTDQSYSDYGIGLAGLM
                     CAKAMDLTFPDLVRSRVIDPGHLLDTYMPPPETEYHRIAHIVGPLAEETDGAMYNSAH
                     ARNLAHPAFGTIASARDLLQFALLFDPIRSRSLFSRVATTTMITDQTGYDFPDAVTAP
                     MSPAVRAWGLGFMVKGRMGFPELCSPAAFGHPGATGCMVLVDPTYDVAFAFVSNRHLN
                     TGYDAWHDRLMMITNVVMAGMTRG"
     gene            1156..>1744
                     /locus_tag="VHR64_04415"
     CDS             1156..>1744
                     /locus_tag="VHR64_04415"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEX3302101.1"
                     /translation="MRRFGLGERVLKTALAAGLSWLLASLIPGNDHPYLAPLSAVLLM
                     QLTIAQSVDLAIQRNIGIAIGVIVAVASFALFGVHAWSVSLVVLISLAAGIQLRLGQQ
                     AVQQVAVSALIVLLAGNVTGTFEYAFRRILDSIIGASVALVLNWLVVPPIHIAPASKA
                     IERMAQDLSLALADLAVSLRSGMTRTRAEAHLKSAR"
BASE COUNT          269 a          612 c          543 g          320 t
ORIGIN      
        1 ttctgtgggt cgcctggacg cggcagttga gttggttgat cgcttcatta cggaaggcgt
       61 cccgggcgcc gggctggtcg tcgccgagca tggcgagatc gtcagcgagt actacgccgg
      121 catggcgcga ctggaccgcc cggccgacgc cgagacactc tggccactgg cctcgatttc
      181 caagctgtac gccgcagccg gctgcatggc cgcgatcgaa gagggtcgga tcgcgctcag
      241 caccaaggcc gcgcagattt tcccggagtt cacgcaaggc gggcgcggca agatcacgct
      301 tcgtcatctc ctgacccaca catcgggtct cccctacgaa tcggaccaga tggccgaccg
      361 cctggccgcg aagatctcac tcgaggagat ggtgcgggaa tcgtacgagg gcgacctgct
      421 cttcactccg ggcaccgatc agtcgtacag cgactacgga atcggtctcg ccgggctgat
      481 gtgcgcgaag gcgatggacc tgacattccc cgatctggtc cgcagccgcg tgatcgatcc
      541 cggccacctg ctcgacacat acatgccacc gccggagacc gagtaccacc ggatcgccca
      601 catcgtcgga ccgctggccg aagagaccga cggcgcgatg tacaactcgg cccacgcccg
      661 caacctcgcc caccccgctt tcggcacgat cgccagcgcg cgcgacttgc ttcagttcgc
      721 tctcctgttc gatccgatcc gttcccggtc gctcttctcg cgcgtcgcga ccacgaccat
      781 gatcaccgat cagaccggct acgacttccc cgacgccgtg accgcgccga tgtcgccggc
      841 ggtgcgcgcc tggggactcg gcttcatggt caagggccgg atgggcttcc cggagctctg
      901 ctcgccggcg gccttcggtc atcccggcgc gaccggctgc atggtgctgg tcgacccgac
      961 ctacgacgtc gcgttcgcct tcgtctcgaa ccggcacctc aataccggct acgatgcatg
     1021 gcacgatcgt ttgatgatga tcacgaacgt cgtgatggct ggtatgactc gaggataacg
     1081 ccgctgaacc ggttgcctcc tgccccgaaa ccgggacctg agtccgacct gctgctgtcg
     1141 caagtaatcc ggcagatgcg gcggttcggg ctgggcgagc gtgtcctcaa gacggcgctg
     1201 gccgccggtc tctcctggct actcgcctcg ctgatacccg gcaacgatca tccgtatctc
     1261 gccccgctca gcgccgtcct cctgatgcag ttgacgatcg cgcagtcggt cgatctggcc
     1321 atccagcgca acatcgggat cgcgatcggc gtgatcgtgg ccgtcgcgtc cttcgcgctc
     1381 tttggcgtgc atgcctggtc ggtcagcctg gtcgtgctga tatccctggc ggccgggatc
     1441 cagcttcgcc tcggccagca agcggtgcag caggtcgccg tttcggcgct gatcgtgctc
     1501 ctggccggga acgtgaccgg cacgttcgag tacgcgtttc gccgcatcct cgactcgatc
     1561 atcggcgcct ccgtggcgct ggtgctgaac tggctggtcg tcccaccaat ccacatcgcc
     1621 ccggccagca aggcgatcga gcggatggcg caggatctgt cgctggccct cgccgatctt
     1681 gccgtcagcc ttcgctcggg catgacccgc acgcgcgcgg aggcccatct caagagcgcc
     1741 cggg
//
LOCUS       DATAOZ010000162         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1910850, whole genome shotgun sequence.
ACCESSION   DATAOZ010000162 DATAOZ010000000
VERSION     DATAOZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910850"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..>2731)
                     /locus_tag="VHR64_04420"
     CDS             complement(135..>2731)
                     /locus_tag="VHR64_04420"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX3302102.1"
                     /translation="GIVGGCCGSSPEHVRQIVQQVQSRPAVARPIQPPATIASMIRAV
                     TMQQEPAPLIVGERVNTQGSRKVKRFALENDFDGLVGVALNQMEEGAHAIDVCMALTE
                     RADEAETMRTLVKKLALNIEAPLMIDSTEAEVVRVALEQNPGRSIVNSINMENGRERI
                     EAVMPHVVKHGAAVVALTIDQAGMAKTRERKLEVAKAIHKIVTEEYGLHPESLIFDAL
                     TFTLATGDPEFNESAIETIEGIRLIEQELPGVMTSLGVSNVSFGLSPAARRVLNAVFL
                     YYCVKAGLDMAIIHPSHVVPLGEIPAEERELAEDLIFYRRPDALQRYIEHFEGRTDTG
                     ASNAVDAMADMDVFERLHYRIVYRKKDGVEADIDEAVATRGDAVDVLNNTLLPAMKEV
                     GDKFGAGELILPFVLQSAEVMKKAVGQLENYLEKQEGVSKGSVVLATVYGDVHDIGKN
                     LVNTILTNNGYTVYDLGKQVPVSTIIDKAVEVNATAIGLSALLVSTSKQMPLCVKELH
                     HKGLEFPVIIGGAAINRPYAHRSLFVDEETPYGSGVFYAKDAFEGLALMDTIIRPDER
                     PELVAKTIDQARKTLSRPDRGSFIARADANDTTRSETRVLEAIPAPPFWGVREPTDFG
                     ASDIWPHLDLKTLFRLHWGGKGVKDEAWEDLVENEFMPRLRRMQEEVVETGWMQPRAR
                     YGYFPANSEGNDLIIFDPEDQDRELTRFTFPRQPARERLCLADYFLPLDSGRKDVAVF
                     QIVTMGAVADAYTDMLQAKNEYSESYFAHGLSVQSAEGLAEYLHQRVRCELGLGPETG
                     KRYSWGYPACPDLAQHALVDQLLDVKSIGVEVTNGFQFNPEQTTAAIVIHHPDAKYYA
                     LLRTGGEE"
BASE COUNT          443 a          884 c          872 g          532 t
ORIGIN      
        1 ccacggcagg gcggtggaca gtgccaagcc gccgcgcgag acacacgtgg tcggccaggc
       61 acgttccata tctcacgtcc aggtcgtcaa cgtggcaggg ttaacaccct gcgctacgtt
      121 ctgagggcca gctcttactc ctcgccgccg gttcggagga gggcgtagta cttggcgtcg
      181 gggtggtgga tgacgatggc ggccgtggtc tgctccgggt tgaactggaa cccgttggtc
      241 acctcgacac cgatcgactt gacgtcgagt agctggtcga caagtgcgtg ctgcgcgagg
      301 tcggggcagg ccgggtagcc ccatgagtag cgcttgccgg tttcgggacc gagtcccagc
      361 tcgcagcgga cccgctggtg caggtactcg gccaggccct ccgcgctctg cacgctcaaa
      421 ccgtgggcga agtagctctc gctgtactca ttctttgcct gcagcatgtc tgtatacgca
      481 tcggcgaccg cacccatcgt cacgatctgg aagaccgcga catccttccg gcccgaatcg
      541 agcggcagga aatagtcggc caggcagagc cgttcccggg ccggctgacg agggaaggtg
      601 aaccgggtca gttcgcggtc ctgatcttca ggatcgaaga tgatcaggtc gttgccttcg
      661 gagttggccg gaaagtagcc ataacgagcg cgcggctgca tccagccggt ctcgacgacc
      721 tcctcctgca tccggcgaag gcgcggcatg aactcattct cgaccagatc ctcccacgcc
      781 tcgtccttga cacccttgcc gccccagtgg agccggaaga gtgtcttgag gtccagatgc
      841 ggccagatat cgctcgcgcc gaaatcggtc ggctcccgca cgccccagaa tggcggcgcc
      901 gggattgcct ccagcacccg cgtctctgag cgggtcgtgt cgttcgcatc tgctcgcgcg
      961 atgaacgagc cgcggtccgg ccggctgagc gtcttccgcg cctgatcaat cgttttcgcg
     1021 accagctcgg gccgctcgtc cgggcggatg atcgtatcca tcagcgccag cccctcgaag
     1081 gcgtccttgg catagaagac tcccgatccg tacggagtct cctcgtccac gaacagggag
     1141 cgatgggcat atggccggtt gattgccgcg ccgccgatga tgaccgggaa ctcgagcccc
     1201 ttgtggtgca gctccttgac gcagagcggc atctgcttgc tggtcgagac gaggagcgcg
     1261 gagaggccga tcgcggtcgc attcacctcg acggccttgt cgatgatcgt gctgaccggc
     1321 acctgcttgc cgaggtcgta gacggtgtag ccgttgttgg tcaggatcgt gttgacgagg
     1381 ttcttgccga tgtcgtggac gtcgccatag acggtcgcca gcacgacgct gcccttgctg
     1441 acgccctcct gcttctcaag gtagttctcg agctgcccga cggccttctt catgacttcg
     1501 gccgactgga gcacgaaggg caggatcagc tcgccggcgc cgaatttgtc gccgacctcc
     1561 ttcatagccg gcagcagcgt gttgttcagg acgtcgaccg cgtcgccgcg cgtcgcgacc
     1621 gcctcatcga tgtcggcctc gacgccgtcc ttcttgcggt agacgatccg gtaatgaagc
     1681 cgctcgaaga cgtccatgtc ggccatcgcg tcgacggcgt tggatgcgcc ggtgtcggtc
     1741 cggccctcga aatgctcgat gtagcgctgg agggcatccg ggcggcgata gaagatcagg
     1801 tcttcagcca gctcccgctc ctcggccggg atctcgccga gcgggaccac gtgcgacggg
     1861 tggatgatcg ccatgtccaa ccctgccttg acgcagtagt agaggaagac cgcgttcagc
     1921 acgcgccggg ctgccgggga caacccgaat gagacgttgg agacgcccag gctggtcatc
     1981 acgcccggca gctcctgctc gatcagtcga attccctcga tcgtctcaat ggccgactcg
     2041 ttgaattctg ggtcgccggt cgccagggtg aaggtcaggg cgtcgaagat caggctctcc
     2101 ggatgcagtc cgtattcttc ggtcacgatc ttgtggatcg ccttcgcgac ttcgagctta
     2161 cgctcgcgag tcttggccat gcccgcctga tcgatggtca gggcgacgac cgctgcaccg
     2221 tgcttgacga cgtgcggcat gacggcctca atccgctccc ggccgttctc catgttgatc
     2281 gagttgacga tcgagcggcc cgggttctgc tccagagcga cccggacaac ctccgcctcg
     2341 gtcgaatcga tcatcaatgg cgcttcgatg ttgagagcca gcttcttgac cagcgtgcgc
     2401 atggtctcgg cttcatcagc ccgctcagtc agcgccatgc agacgtcgat cgcgtgcgcg
     2461 ccctcttcca tctggttcaa agcgacgccg acgagcccat cgaagtcgtt ctcgagcgcg
     2521 aaccgcttga ccttgcgcga gccctgcgtg ttgacccgct cgccgacgat gagtggagcc
     2581 ggctcctgct gcattgtcac cgcgcgaatc atcgacgcga tcgttgctgg cggctgaatc
     2641 ggcctggcca ccgccgggcg gctctggact tgctggacta tttggcggac gtgctcgggg
     2701 ctcgagccgc agcacccgcc gacgatgccg a
//
LOCUS       DATAOZ010000163         5714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7698131, whole genome shotgun sequence.
ACCESSION   DATAOZ010000163 DATAOZ010000000
VERSION     DATAOZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7698131"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..419)
                     /locus_tag="VHR64_04425"
     CDS             complement(93..419)
                     /locus_tag="VHR64_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302103.1"
                     /translation="MLPSTPNDGSVELDRALHLKTAPQPFAWQNGSQLLLLDVEGPQW
                     LVAELAFNADGCIYTEVRRASYDSQREAIGALLSRALSCGDDALIDTVERLNGYMTEH
                     YQISLI"
     gene            653..1495
                     /locus_tag="VHR64_04430"
     CDS             653..1495
                     /locus_tag="VHR64_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX3302104.1"
                     /translation="MPDRPKEPKTNGAGSNKREPLIPNPTINVAARTGRPTEDSLLFA
                     WDQELRERAAAFTHSDTWRVLRIMGEFVAGFDALAEVGPAVTIFGSARVAPSHPMYKA
                     AREVGKRLAKAGLATITGGGPGIMEAANRGAAEAGGVSIGANIELPMEQGVNRWVNVP
                     LNFRYFFVRKTMFVKYAEGFIIFPGGYGTLDELFEALTLIQTGKLWNFPIVLFDTAYW
                     SGLVDWIESRLTAEGMIKPEDRNLFMVTDDPQAAVDVMLDSYRRHSEQRLFEETDRVP
                     GDLR"
     gene            1496..2644
                     /locus_tag="VHR64_04435"
     CDS             1496..2644
                     /locus_tag="VHR64_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX3302105.1"
                     /translation="MGATVIPTKQNGDTLAVRFIAESDQTAADVAGQMAEFISLARHR
                     LDLAVYDCRLDEGAAGSIRDALQDRLKHGVQLRLIYDDSAKKPQTHDQFESIGGDFAE
                     GGTHERVEELGLPNELIRGIRGEGLMHEKFIARDGEAVWTGSMNWTNDSMTRMENTIV
                     SLISPELTALFERDFDQLWSTRQTLESGAFLTSPAELRYAGRSALTDVDFSPGQGEYI
                     NEWVARRVARARRRIIFCSMLINSSKLLGALMKVLDEQHLELWGVYDKTQMEGVLQQW
                     NGQPQVQWKVEAVQRLLREAELIGKESLPYRPGHSHNFMHNKLLIVDDTVITGSYNLS
                     HAAQSNAENMLAIESPALAEDAISYVRMLRDRFLDSPNGDNPKPRDRR"
     gene            2924..5380
                     /gene="lon"
                     /locus_tag="VHR64_04440"
     CDS             2924..5380
                     /gene="lon"
                     /locus_tag="VHR64_04440"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX3302106.1"
                     /translation="MTRYEPYRTRYPLLPLKNIVIFPRNIVTLLVGRPRSIQAVEEAL
                     NRDRRVVVVAHRDPDIDDPLASDVYNVGTLAEVVKSERQQATTLQVVLEGICRVRLTS
                     FDTSRPFFNVAAEELTEIEPPVEESRTLMKHVTELACTYSESRNKLTNEVLDMVQRAS
                     DPGHLADLLATQLLSDTAKRQELLEEVKPYERLVRVAGLIQSDLDIAALEQRIKDRVR
                     DQVEKNQREYYLREQLKAIHDELGGDGGSEMDNLRRKIAESGMPDEVAEKLSRELLRL
                     ERMPAVSAEATVVRTYIDTMISLPWTNQSEDRLDLEIAEQILDEDHYGLEHVKERILD
                     FLAVRKLRQASNQVGLGVSQILCLAGPPGVGKTSLGRSIARSMNREFVRVSLGGVRDE
                     AEIRGHRRTYIGAMPGRMITAMKQAGTINPVILLDEIDKLSSDYRGDPASALLEVLDP
                     EQNRNFVDHFLDVQYDLSNVLFITTANYLGQIPRPLRDRMEIIEIGGYTEDEKAEIGR
                     RYLLTRNMTAHGLPEGSLEIPHKVWVDLVRDYTREAGVRELDRSVATLCRKTAREVVR
                     GKSGKVRITKTRLEELMGPKQYGMEVTTEGSQVGTAMGLGTTEIGGELIPVEVLTMPG
                     KGGLTITGRAGEVMQESAQAALSYARSRTHHLRIDPDFRERLDLHIHLPETAQPKDGP
                     SAGITMATALISALTKRPVRADTAMTGEITLRGRVLPIGGLKDKTLAAHRCGIRRLIA
                     PAQNRRDFEKVPTNVAKEMTFIWVRDMDEVIQAAIMLDVDQVGNLSTAGDEIPADAIA
                     PATGFGTQVVTDAAPNQIKG"
     gene            complement(5365..>5714)
                     /locus_tag="VHR64_04445"
     CDS             complement(5365..>5714)
                     /locus_tag="VHR64_04445"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302107.1"
                     /translation="ELQIEADIDDEPVTLGLVDRIDHLGPFGAGNERPLLRWRGVQIS
                     RWAEVGKDKTHAHVWLSKGNGSIRTIFFGGAQRMRELGPMQRVDALVELSVNVWNQTR
                     SVDAKLVDVQPLI"
BASE COUNT         1134 a         1827 c         1786 g          967 t
ORIGIN      
        1 ggaatcgaat acgccaatcg gcgcggaggg gtgtacgttc atacccccaa tgctatcact
       61 cgtatgttgg cttgagaaag aggtggaacg ggctagatca ggctgatctg gtaatgctcg
      121 gtcatataac cgttcagccg ctcgaccgtg tcgatcaagg cgtcgtcgcc acatgagagg
      181 gcgcggctga ggagggcgcc gattgcctcg cgctgcgagt cgtaagaggc acggcgaact
      241 tcggtataga tgcaaccgtc cgcattgaag gcgagctcag cgaccagcca ttgcggacct
      301 tcgacgtcca ggagcaagag ctggcttccg ttctgccagg caaacggctg gggcgctgtc
      361 ttgaggtgga gcgcgcggtc cagctcgacg ctgccatcgt tcggagtgga tggcaacatg
      421 gggtgctctc gagatgatcg cgcaaacgcc gcgtgatcca tagcaatgcc ctgcgtcaga
      481 gacattccgg ggcagaacgt cgactgcggt aaccggcttc ggttcgtgtc cgacaggcaa
      541 agcgtatcac cgaacctacc cccggcacaa tggtccgtcg ggttgtacgg ggtacatttc
      601 catcaatagt cctgccagct acaacggtgc aaactggaga atgtgcgtcg aaatgccaga
      661 cagacctaag gaaccgaaaa ccaacggagc cgggagcaac aagcgagagc ctctcatccc
      721 caacccgacc atcaacgtcg ccgcacgcac cggacggccc accgaggaca gtttgctctt
      781 cgcgtgggac caggagctgc gggaacgcgc ggcagccttt acccacagcg atacctggcg
      841 cgtgttgcgg atcatgggcg agttcgtggc cggttttgac gcgctagccg aggtcggacc
      901 ggcggtgacg atcttcggct ctgccagggt cgcgccgagt cacccgatgt acaaggccgc
      961 gcgggaggtc ggcaaacggc tggcgaaagc cgggctggcg acgattaccg gcggtggccc
     1021 cggcatcatg gaggcggcga atcgcggcgc ggccgaggcg ggaggagtct cgattggcgc
     1081 taacatcgag ctgccgatgg agcagggcgt caaccggtgg gtgaatgtgc cgctcaactt
     1141 ccgctacttc ttcgtgcgta agacgatgtt cgtcaagtac gccgagggat tcatcatctt
     1201 ccccggcggt tacggcacgc tcgacgagct gttcgaggcg ctgacgctga tccagaccgg
     1261 caagctctgg aacttcccga tcgtcctctt cgacaccgcg tactggtctg gactggtcga
     1321 ttggatcgaa tcgaggctga cggccgaagg catgatcaaa cccgaggacc gcaacctgtt
     1381 catggtgacc gatgatccgc aggccgcggt cgacgtgatg ctcgattcct accggcgcca
     1441 ctccgagcaa cgactgttcg aggagaccga tcgcgtgcct ggggatctgc gctagatggg
     1501 cgcgacggtc attcccacaa agcagaacgg cgacacgctc gcggtccgct tcattgccga
     1561 gagcgatcag acggccgccg acgttgctgg gcaaatggcg gagttcatct ccctcgcgag
     1621 acatcgcctc gacctggccg tctacgactg tcgcctcgac gagggcgcgg ccggctcgat
     1681 ccgcgacgcc ctccaggatc ggcttaaaca cggcgtccag ctccggctga tctacgacga
     1741 cagcgccaag aagccgcaga cgcacgacca gttcgagtcg attggcggcg acttcgccga
     1801 aggcggcacg catgagcgcg tcgaggagct cggcctgccc aatgagctga tccgcggcat
     1861 acggggcgag ggcctaatgc acgagaagtt catcgcgcgg gacggcgagg cggtttggac
     1921 cggttcgatg aactggacga atgactccat gacgcggatg gagaatacga tcgtcagcct
     1981 catctcgccg gagctgaccg ccctctttga gcgcgacttc gaccagctct ggagcacgcg
     2041 gcagacgctc gaatcgggcg cgttcctgac gtcgccggcc gagctcaggt acgccggacg
     2101 atctgccctg accgatgtcg acttctcgcc cggccagggc gagtacatca acgaatgggt
     2161 cgcgcggcgc gttgcccgcg cccgccggcg gatcatcttc tgttcgatgc tgatcaactc
     2221 gagcaagctg ctcggcgcgc tgatgaaggt gctcgacgag cagcatctcg agctctgggg
     2281 cgtctacgac aagacccaga tggagggagt cctccagcaa tggaacggtc agccgcaggt
     2341 ccagtggaaa gtggaagccg tccagcgcct gctccgcgag gccgagttga tcggcaagga
     2401 gagcctgccg tatcggcccg gacattccca caacttcatg cacaacaagt tgctgatcgt
     2461 ggacgacacc gtcatcaccg gctcgtacaa cctgtcgcac gccgcacagt ccaacgccga
     2521 gaacatgctg gcgatcgaga gtccggcact cgccgaagat gccatctcat acgtccggat
     2581 gcttcgagac cgattcctcg atagtccaaa tggcgacaat ccgaagcccc gggatcggcg
     2641 ttgagatcga aacggcgact cttgggctag cgtgcacgct tgacgaatcg ccagttcgcg
     2701 ggatagtgga agtgcccgta taagtaaacc cgcccgaaga tttgtggtgc gcggtaacat
     2761 gtgccccttg gtcacggata tctgtctgcg gtatcatcgc cgccggcaga ctgataagtg
     2821 aatctccccc cgcttggaga tcatcagtac cccgcttcag tggctgatcg gcgtacacag
     2881 tgatgttgtt gcacgcaacg atggagcggt tgaggaccaa tttatgacgc gctatgaacc
     2941 gtacagaact cgctatcccc tgctgccgct caagaacatc gtcattttcc cgcgcaacat
     3001 cgtgacgctc ctggtcggcc gaccgcggtc gatccaggcc gttgaggaag ccctcaaccg
     3061 ggatcgccgc gtcgtcgttg tcgctcaccg cgatccggac atcgatgacc cactggcaag
     3121 tgacgtctat aacgtcggta cgctggcaga ggtcgtcaag agcgagcggc agcaggcaac
     3181 gacgctgcag gtcgtgctcg agggcatctg tcgggtccgc ttgacctcct tcgacacgtc
     3241 gcgaccgttc ttcaacgtcg ccgccgaaga actgactgag attgagccgc cggtcgagga
     3301 aagccggacg ctcatgaagc acgtcaccga gctcgcctgc acctactccg aatcccgcaa
     3361 caagctcacc aacgaagtgc tggacatggt ccagcgcgcg agcgatcccg ggcatctcgc
     3421 cgatctgctc gcgacccagc ttctctcgga caccgccaag cgccaggagc tcctcgaaga
     3481 ggtcaagccc tacgagcgcc tcgtccgcgt tgccggtctc atccagagcg acctcgatat
     3541 tgccgcgctc gaacagcgca tcaaggaccg cgtccgcgac caggtcgaga aaaaccagcg
     3601 cgagtactac ctgcgcgagc aactcaaggc gatccacgat gagctcggcg gagacggcgg
     3661 cagcgagatg gacaatctcc gtcgcaagat cgccgaaagt ggcatgccgg atgaggtcgc
     3721 agagaagctc agtcgcgagc tcctgcgact cgaacggatg ccggccgtct cggccgaggc
     3781 aaccgtcgtt cgcacctata tcgacacgat gatcagcctg ccctggacga accagagtga
     3841 ggatcgcctc gacctcgaaa tcgccgagca gatcctcgac gaggaccatt acggtctcga
     3901 gcacgtcaag gagcgcatcc tcgacttcct ggccgtgcgc aagttgcgcc aggccagtaa
     3961 ccaggtcggt ctcggcgttt cgcagatcct ctgcctggcc ggacctcccg gtgtcggcaa
     4021 gacaagcctc gggcgatcga tcgcccgctc gatgaaccgc gagtttgtcc gggtcagtct
     4081 cggcggcgtc cgcgacgaag ctgagattcg cggccaccgt cgcacctaca tcggcgcgat
     4141 gcccggccgc atgatcactg ccatgaagca ggccgggacg atcaacccgg tcatcctgct
     4201 cgacgagatc gacaagctct cctccgacta ccgcggtgat ccggcgtcgg ctctgctcga
     4261 ggtgctcgac ccggagcaga accggaactt cgtcgaccat ttcctcgatg tgcagtacga
     4321 cctctcgaac gtgctcttca tcacgaccgc caactacctt gggcaaatcc cgcggccgct
     4381 gcgcgaccgc atggagatca tcgaaatcgg cggctacacc gaggacgaga aggcggagat
     4441 cggccgtcgg tacctcctga ccaggaacat gaccgcccac ggcttgcccg agggatcgct
     4501 cgagatcccg cacaaggtct gggtcgacct cgtccgcgat tacacccggg aagccggtgt
     4561 gcgcgagctg gaccgatcgg tcgcaaccct ctgccgaaag acggcccggg aggtcgtccg
     4621 cggcaagtcg ggcaaggtcc gcattacaaa gaccaggctc gaggagctga tgggcccgaa
     4681 gcagtacggg atggaggtca cgaccgaggg ctcccaggtc ggcaccgcga tgggtctcgg
     4741 cacgaccgag atcggcggcg agttgattcc ggtcgaggtc ctgaccatgc ccggcaaggg
     4801 cgggctgacg atcacgggtc gtgccggcga ggtcatgcag gagtcggcgc aggccgcgct
     4861 ctcctatgcc cgctcccgga cgcaccacct gcggatcgat ccggatttcc gtgagcgtct
     4921 ggacctgcac atccacctgc cggagaccgc gcagcccaag gacgggccgt cggccggtat
     4981 caccatggcc actgccctta tctctgcgct caccaaacga cccgtgcggg ccgatacggc
     5041 catgaccggg gagatcaccc tccgcggccg ggttctgccc atcggcggcc tcaaggacaa
     5101 gacgctggca gcccaccgct gcggcatccg tcgcctgatc gcgccagcgc agaaccggcg
     5161 ggacttcgaa aaggtcccaa ccaatgtggc caaggagatg accttcatct gggtgcggga
     5221 catggacgag gtgatccagg cggcgatcat gctcgatgtc gatcaggtcg gcaacctcag
     5281 cactgccggg gatgagattc cggccgacgc gattgcaccg gccaccggct tcggcacgca
     5341 ggtcgtgacc gacgccgcgc caaatcagat caagggctga acgtcgacca gtttcgcgtc
     5401 gacgctccga gtctgattcc agacgttgac tgagagctcg accagcgcgt cgacccgttg
     5461 catcgggccg agctcgcgca tgcgctgcgc cccgccgaag aagatggtgc ggatcgatcc
     5521 attgcccttg ctcaaccata cgtgagcgtg ggtcttgtcc ttgccgacct cggcccagcg
     5581 gctgatctgc accccacgcc agcgaagcaa cggtcgctcg ttgcccgcgc cgaacgggcc
     5641 gagatgatcg atccggtcga ctaacccgag ggtgacgggc tcgtcgtcaa tatccgcctc
     5701 aatctgcagc tccg
//
LOCUS       DATAOZ010000164        11008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2699776, whole genome shotgun sequence.
ACCESSION   DATAOZ010000164 DATAOZ010000000
VERSION     DATAOZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11008
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2699776"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VHR64_04450"
     CDS             <1..867
                     /locus_tag="VHR64_04450"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX3302108.1"
                     /translation="DTATEAADETDGPAPGPDVEPESLQWAEATGALVLGVLAPDDAA
                     AAAQEIARSATLQAEVATLLPVADILLSLYQQTQPVPTVTATTAPVAAPAAERTPAEP
                     ARRVERPRAEPITRPAMPSLGGIRPSSILIGLFALIAAIGLLWALSLNDRIATKDDEI
                     TALKRQVEQFRQAANASAFTLTPTASAPDGARGTVFYSLADSRVIIDVSGLPELDQDR
                     VYQVWFQRTGSNAWEPGPTFLVNQQGESVQRLPGETPTFARIAVSEEPAPGSAEPSGD
                     FLLEGALSGGNG"
     gene            1045..2658
                     /locus_tag="VHR64_04455"
     CDS             1045..2658
                     /locus_tag="VHR64_04455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3302109.1"
                     /translation="MEQAPWRWRLPASVRTSPFFWISLGSLISFLLTWRLFNLPMVSD
                     EGGYAYAAERWFNGRGRLYHDIWISRPQGIFVVYGTVIKFLGGSTLDLRLVAWAFNIL
                     TMIAVWMIARHWRGPGVAAGAVMIFAVMMGSPAIEGFTANAEAFMAVPAAFAIITLIR
                     AWERSWSPPWLAVTGLLIGIATQLKPSGATSLFVVVGFIAVMTVDTDHFVRLTTRRSL
                     PVMLGFGASLLPAIYHGYRIGFDAFVYGSVGYRITRQSSATGSLHHHVRALGDLSARI
                     WPIYILLGLIALAWFLTSDTRHQPPRRASRPVRRELRPLPALGILPRQFLVESADPMR
                     RLLQIWIVGCLFGISMGGDWWYHYLIQIMAPFSIWLAVSMADLRPRLPRLAHIGLAVS
                     IAVLLLVPYSVITKPTRAAMTSPLFGHPGYADQEVVAKYLHDHTAPETPIFVAFDQAA
                     LYYLADRPSTYRYLYDQELRAFPDSEAKLVEMVEGPNRPLYIVGTRQLAPFADRGLAF
                     WEAVQRHYVLETVVRGVPIYRAIDEPATP"
     gene            2661..3110
                     /locus_tag="VHR64_04460"
     CDS             2661..3110
                     /locus_tag="VHR64_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3302110.1"
                     /translation="MSEVSIRPFTSADQAAVRALILAGLQDHWGTLDPTLNPDLNDIA
                     SWYGPLGGFTIVAEIDNEIVGTGTMHRLDDQTGVLVRMSVSGAHRGKGIGKALVRSLA
                     DEARSRGYTHLICETTDTWQDAIALYLATGFTIVDQRNGDYHFELPL"
     gene            complement(3252..3917)
                     /locus_tag="VHR64_04465"
     CDS             complement(3252..3917)
                     /locus_tag="VHR64_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX3302111.1"
                     /translation="MLLANLREQVIEAGLEAHRRGIVYGTAGNFSIRDRETGLIAISP
                     SGMPYGSMTPADVCIVTEDAGIEDACRRPSSETPMHTMIMKKRTDVTAIVHTHSHYCT
                     VVSTFKPRLPPILTETVLVLGSRIPVTRYGETGTPDIGLSVVEVMDETTRAVIMKNHG
                     LICFGESFEQALTYAMIAEEAAHVYIDALAANGGREPDYVPEHLIEKMTAHFRATYGQ
                     IKS"
     gene            4065..4850
                     /locus_tag="VHR64_04470"
     CDS             4065..4850
                     /locus_tag="VHR64_04470"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3302112.1"
                     /translation="MDRSCRFDGKSVIVTGAGMGIGKGAAIAFAREAGCVIVADISAS
                     AADNTVREITSQGGKAISVTCDVSVSDAVQRMVAAGVKAFGGIDVVFNNAGINLYGRV
                     DEMSEADWDRQIAVNLKSVFLVSKYAIPEMRKRGGGAIVNNASVQAFASQRTVAAYAA
                     TKGAIVSLTNVMALDHAPDNIRVNCIAPGSIRTPLLENSAQTFAPDDPDSAIVEWGKD
                     HPIGRIGTVEDVANLVLFLASDEASFCTGACYRVDGGLLSSLM"
     gene            complement(5171..5713)
                     /locus_tag="VHR64_04475"
     CDS             complement(5171..5713)
                     /locus_tag="VHR64_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX3302113.1"
                     /translation="MNHSDRINVIEMLRGLRQTRSYSSEPVDDATLDAILEVARWTGS
                     GGNKQPWRLVVVRNAETRRAIAGAKSDTGWVADAPVVIGIVTEGKTAESARFDAGRLV
                     ERTMLAAQGSGLAAAVIGFGAADSEPAMTVRRLLNVPEGMWITHAVVIGHPGQKSAES
                     GSKRGGRKPMSEIMVEERFG"
     gene            complement(5887..6810)
                     /locus_tag="VHR64_04480"
     CDS             complement(5887..6810)
                     /locus_tag="VHR64_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302114.1"
                     /translation="MSATTTTLADINVRQTERATRKPQGLVRRALGTFARDRVAMTAL
                     VVVLLLVIFALGAGVVSRLTGFTPQENHLAAKLSAPGENGYILGSDANGRDILTRLAY
                     GARISLTVAVLATFFELTIGLGVGLISGYAGKWVDFVLMRLVDVLLSIPTLPLLILIS
                     TLYRPGTYMLALILALVSWPGDSRLLRGEALSLKSREYVDAAKVVGVSPVGIVLRYIL
                     PNVMPTMLVLASLTVPALIIAETSLSFLGVGVQVPTPSWGNMLDEAYRFYRTNWTNVF
                     FPGFIVFSASLALYLVGDGLRDALDPKQSPR"
     gene            complement(6807..7790)
                     /locus_tag="VHR64_04485"
     CDS             complement(6807..7790)
                     /locus_tag="VHR64_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302115.1"
                     /translation="MLRFVIRRILIMIPLLFGVSFISFALMNLIPGSPVAMTARSNPK
                     IRQEDINRIREQLGLNDPWPQRYVEWLRDVVFHLDFGPSFYNRLPVTDRIWAAMPNTL
                     LLTSAALLFSLAVSIPFGIYSAVYHKRAFDRLVNIFSVALYSIPLFWLGILLIILFSV
                     QSNRWDLWWFPTLPSGGIKSPRGGGGATDRLEHLILPMITLASFQIGVWTAYIRSSML
                     EALGQDYIRTARSKGVRELRVLYGHAFRNALLTLTTLVGLSIPGLFGGALLIEYIFAW
                     PGMGSLTIEAVGRRDYTMVQATTLLFAFLTMIGNMLADILYGLIDPRVRVS"
     gene            complement(7852..9636)
                     /locus_tag="VHR64_04490"
     CDS             complement(7852..9636)
                     /locus_tag="VHR64_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3302116.1"
                     /translation="MRLRLTWAGRVLAIFMLFSMIGLPIASAQDASPEASPVTGESTP
                     GASPIASPEASPVAASCEPGPSKTSADIRQEIEEQFPIEQPTNTEGTYVTGSVGDLQS
                     VNPYIVEEQTSLAVVGLVFDSFLGGDPDTGQPVPGGVTDYWELAEDCVTYTFHLRTDK
                     VWQDGVDLTADDVAFSFESLADPVLASPYTGQFLDAVASWKVIDADTIQVVSNGVRAD
                     FLNNAGFIIIPKHIWESIPRDQWATDPGSTGQDPSRVVGTGPFKFESWTQGQEIHLVR
                     NEQYTPRPAWIKDIVLRIFADTEAQLNAFLTGELDDNGLEPEQVETVKADPQFTVEDF
                     PYRGFTYYEFNLDPEVTTRFQDVRVRQAFMYALDRQSIVDNILLGYGEVANGTQPSIS
                     YAYAPDQINTVYTYDPEKAKQLLTEAGWTDTNGNGTVDKDGIEMDFEFLYPSGSATTD
                     TMVAYFQDAWKAVGIGIKPVAMEFPALIEATTTNPTFEMALYSFGWDSTFIQDAMFGC
                     DQYQVGFNDMKYCNPELDVLGEQIKVTVDQEERARLMIQYSNIVNDEQPVGIINFAVS
                     IVAWNNRMHNLFPGPWGGPGIEYLWIEQ"
     gene            9879..>11008
                     /locus_tag="VHR64_04495"
     CDS             9879..>11008
                     /locus_tag="VHR64_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3302117.1"
                     /translation="MGENTQVRQLYRDALSGKLNRREVFQRGIALGLSANVIGMLAIN
                     AVKMPTAFAATEGKPSATFYDWMLNLHPRITSLGEEMGVDIQTAPTENFGNDRFIAEG
                     LQKNSTWDFYGGVTPFLEMIGLVDSGTIEPWTQYLPAGFTDDFVPATLTEGTYNGEFY
                     VWPLLLDICVQMRHAGIVQKAGLDPEASPATWDEFIANGQKVVDSGAAPYGIVFDHRD
                     WRSLIPVTHSISIDVYDKETGLFQYASDPALEALEILKRMMPLAPADLLTADAVDNTV
                     HPDEAAFAAEQVGYYFKYQNAGFQFSANWPDPSQLRLGGLPKTENGVGGSVFWDTGAV
                     LFTYGANKPKAVEYLTALSKDQEMWKDSITGNADQGTIPVGQL"
BASE COUNT         1985 a         3679 c         3408 g         1936 t
ORIGIN      
        1 gataccgcga cggaggctgc tgacgagacg gacggtccgg cgccgggacc cgatgtggag
       61 cccgagtcgc tccaatgggc cgaggcgacc ggcgccctcg tgcttggcgt cctcgcgccg
      121 gacgacgccg cagcggccgc gcaggaaatc gcccgctcgg cgacgctgca agcagaggtc
      181 gctacgctcc tgccggtggc cgatatcctg ctcagcctct atcagcagac gcagccggtg
      241 ccgaccgtaa ctgcaactac cgcaccggtc gccgcgccgg cggctgagcg cacacccgct
      301 gagcctgccc ggcgggttga gcggccccgt gcggagccga tcacccgccc ggccatgccc
      361 agcctgggcg gcattagacc gtcgtcgatc ctgatcggcc tgttcgcgct cattgccgcg
      421 atcggtctcc tctgggcgct ctcgctgaac gaccgcatcg cgacgaagga cgacgagatc
      481 accgccctca agcgacaggt cgagcagttc cgccaggctg ccaacgcgag cgcctttacc
      541 ctgaccccga ccgccagcgc accggacggc gcccgcggca ccgtcttcta ctcgctggcc
      601 gattcccggg tgatcatcga cgtctcaggc ttgcccgagc tggaccagga ccgcgtctac
      661 caggtctggt tccagcgcac tgggagcaac gcctgggaac cggggccaac cttccttgtc
      721 aaccagcagg gcgagtcggt ccagcgcttg ccgggcgaga cgccgacctt tgcccggatc
      781 gcggtttccg aggagccggc gccgggcagc gcggagccga gcggagactt ccttcttgaa
      841 ggcgcgctgt ccggcggaaa cggctgatag caccggacga ttggaaccaa tttgggccgc
      901 ggcggcgtac ataatttggt ggcgtgaggc acaatgggga cgtaaccccg gcgtatcatt
      961 gcccggatat tcgccggagc tttccgtcgc ccgcactccg agttaagtcg aacagcgagt
     1021 caattgagga gacgactgcg cacaatggag caagctccgt ggcggtggcg attgccagcg
     1081 tccgtgcgaa cgtctccctt tttctggatc agcctcgggt cgctgatctc cttcctgctc
     1141 acctggcggc tctttaacct gcccatggtc tccgacgaag ggggctacgc ctatgccgcc
     1201 gaacgctggt tcaatggccg gggccgcctc tatcacgata tctggattag ccggccgcag
     1261 ggcatcttcg tggtctatgg aaccgtcatc aagttcctcg gcggcagcac gctcgatctt
     1321 cgcctggtcg cctgggcatt caacatcctg acgatgatcg ccgtctggat gatcgcccgg
     1381 cactggcgcg gtcccggggt agcggccggc gcggtcatga tcttcgcggt gatgatgggg
     1441 tcgccggcaa ttgagggctt caccgctaat gccgaagcct tcatggccgt cccggccgcg
     1501 tttgcgatca tcacgctgat ccgcgcctgg gagcgaagct ggagcccgcc ttggctcgct
     1561 gtgaccggtc tgctgatcgg gatcgcgacg cagctcaagc cctccggcgc cacatccctg
     1621 ttcgtggtcg tcggcttcat tgccgtcatg accgtcgaca cggaccactt cgtgcggctg
     1681 acgacgcgac gcagcctgcc cgtcatgctc ggcttcggcg catcgcttct ccccgcgatc
     1741 taccacggct accggatcgg cttcgacgcc ttcgtctatg gatcggtcgg gtaccggatt
     1801 acccggcaga gttcggcgac cggatcgctc caccaccacg tgcgggcgct gggcgatctc
     1861 tcggccagga tctggccgat ctacatcctg ctcggcctga tcgccctggc ctggttcctg
     1921 acgtcggata cccggcatca gccaccgcgc cgcgccagtc gaccggtccg ccgcgagttg
     1981 cggccgctac cggcgctcgg catccttccg cgccaattcc tcgtcgaatc cgctgatccg
     2041 atgcgccgat tgctccagat ctggatcgtc ggctgcctgt tcggaatctc gatgggcggc
     2101 gactggtggt accactacct gatccagatc atggccccgt tctcgatctg gctggcggtc
     2161 tcgatggctg atctgcggcc gcgcctgccg cgtctcgcgc atatcggact ggcggtctcg
     2221 atcgccgtgc ttctgctcgt gccgtactcg gtcatcacca agccgacccg cgcggccatg
     2281 accagcccgc tcttcggtca tccaggctac gccgatcaag aggtcgtcgc gaaatacctg
     2341 cacgatcaca ccgcgcccga aacgccgatc ttcgtcgcct tcgaccaggc cgcgctctac
     2401 tacctggccg accgcccttc gacctatcgc tacctctatg atcaggagtt gcgcgctttc
     2461 cccgactccg aggcgaagct ggtcgaaatg gtcgaaggtc cgaaccggcc gctttacatc
     2521 gtcggcaccc gccaactcgc gccgttcgca gatcgcggcc tcgccttctg ggaggcagtt
     2581 cagcgccatt acgtgctcga gacggtggtt cgcggcgtgc cgatctaccg cgcgatcgac
     2641 gagccggcaa cgccctagcg atgtccgaag tctcgatccg ccccttcacg tcggccgacc
     2701 aggcggcagt ccgcgccctg atactggccg gcttgcagga tcactggggc acgctcgacc
     2761 cgaccctcaa ccccgacctc aatgacatcg cgagctggta cgggccgctc gggggcttca
     2821 cgatcgtggc cgagatcgac aatgaaatcg tcgggaccgg cacgatgcac cgtctcgacg
     2881 accagaccgg cgtcctcgtc cggatgtcgg tttccggcgc gcaccgcggc aaggggatcg
     2941 gcaaggcgct ggtccgctcg ttggccgatg aggcgcgctc ccgcggttac acccacctga
     3001 tctgcgagac gaccgacacg tggcaggacg cgatcgccct ctacctcgcc accggcttca
     3061 ccatcgtcga ccaacgcaac ggcgactacc acttcgagct gccgctctaa tatccgacgg
     3121 cgatcggtac gcgactcaaa cgaagcgcgg ggttaccact ccccgcaccg ttgattcccg
     3181 cacgcgacgt ttcgcagcca gtgcgtgtcc acgtgcggtg atgaggaatt gccggccacg
     3241 ggggctcggc gctacgactt tatctgtccg tacgtggccc ggaagtgcgc agtcatcttc
     3301 tcaatcagat gctccggcac gtaatccggt tcccggccgc cgttcgcggc cagtgcgtcg
     3361 atgtacacgt gggcggcttc ctcggcaatc atcgcgtagg tcagtgcctg ctcgaacgac
     3421 tcgccgaaac agatcaaccc atgatttttc atgatcaccg cgcgggtcgt ctcgtccatc
     3481 acctcgacga cactcaggcc gatatcggga gttccggttt ctccgtatcg cgttacggga
     3541 attcgggaac ccagtacgag cacggtctcg gtcaggatcg gcggcaggcg gggcttgaac
     3601 gtgctgacca cggtgcagta gtgcgaatgg gtatggacga ttgcggtcac gtcggtccgc
     3661 ttcttcatga tcatcgtgtg catcggcgtc tcggagctcg gccgccggca cgcgtcctcg
     3721 atcccggcat cctcggtcac gatgcagaca tcggccggcg tcatcgaccc atacggcatc
     3781 ccgctcggcg agatcgcgat caacccggtc tcccggtcgc ggatgctgaa gttaccagcc
     3841 gtgccgtaga cgatcccccg ccggtgcgcc tccaaccctg cctcgatcac ctgctcgcgc
     3901 aaattcgcca gaagcaccga ttcacccctt atcgccgata ttaatccggt cgtttcgcag
     3961 cagaactcta cgcgccgcaa cccccattgt gccgcccgcg ctggttggga taatacggcc
     4021 acacgcggcg atggctggcg acgcgggtgg gtaaggaggc tgagatggat cggtcatgtc
     4081 ggttcgacgg aaaatcggtc atcgtcaccg gcgccggaat gggcatcggt aagggcgcgg
     4141 cgatcgcctt cgcgcgtgag gctggctgcg tgatcgtggc cgacatctcc gcatcggccg
     4201 cggacaacac cgtccgcgag atcacgagcc agggcggcaa agcgatctcg gtcacctgcg
     4261 acgtttcggt ttccgacgcc gtccagcgga tggtcgcggc cggcgtcaag gccttcggcg
     4321 gcatcgatgt cgtcttcaat aacgccggga tcaacctcta cggccgcgtc gatgagatga
     4381 gcgaggccga ctgggaccgc cagatcgcgg tcaacctcaa gagcgtcttc ctcgtcagca
     4441 agtacgcgat ccccgagatg cgaaagcgcg gtggcggcgc gatcgtcaac aatgcctcgg
     4501 tccaggcctt tgcctcgcag cgcactgtcg cggcttacgc ggccaccaag ggcgcgatcg
     4561 tctcgctgac caacgtgatg gcgctcgatc acgcgccgga caacatccgg gtgaattgca
     4621 tcgcgcccgg ctcaatccgg acgccgctgc tcgagaactc ggcccagacc ttcgccccgg
     4681 acgatcccga ctccgccatc gtcgagtggg gcaaggacca cccgatcggc cggatcggca
     4741 ccgtcgagga cgtggccaac ctcgtcctct tcctggccag cgacgaagcc tccttctgca
     4801 ccggcgcctg ctaccgcgtc gacggcggcc tcctgtcgtc actgatgtag aagagacctc
     4861 ccttctcccg catgcgggac aagggaggtc caacactttc tcccctctcc tgcgttcggg
     4921 agagggaccg ggggtgaggg ccatagaccc acgcgcaccg ggtctcaagc cgtcccggtg
     4981 ttagggaggg tcggtgaatg tgagccggtc tgtcggcgtc gcccggggat atatgtccgg
     5041 cctgaaccac gagaagccca ctgaaggggc taggcggttt atggacgcat cattacagcc
     5101 ccttcggtgg gctcttgtca ggtcagcccc gacctttagc tcggggcggc acccggatgc
     5161 aagcgatcgg ttacccgaac cgctcctcga ccatgatctc cgacatcggc ttgcgaccgc
     5221 cgcgcttgct gccactctcc gccgacttct gaccgggatg accaatcacg accgcgtgcg
     5281 tgatccacat cccctccggc acattcagca accgccgaac agtcatcgcc ggttccgaat
     5341 cggccgcacc gaacccgatc accgccgccg caagaccgct tccctgagcc gcgagcatcg
     5401 tccgctcgac cagtcgcccg gcgtcgaacc gggctgattc cgccgtcttg ccttcggtca
     5461 cgatgccaat cacgaccggc gcatcggcga cccagccggt atccgatttc gccccggcaa
     5521 tcgcccggcg cgtctccgca ttccgcacga cgaccagccg ccacggctgc ttgttgccgc
     5581 cgctgccggt ccaccgcgcg acttcgagga tcgcgtcgag cgtggcgtca tccaccggct
     5641 cgctggaata cgagcgcgtc tgccgcaacc cgcgcagcat ctcaatcacg tttattcgat
     5701 ctgaatgatt cataggcgcc tcctcatgca cgaatcggaa acgtagcgcg gggttaccat
     5761 gccccgccct ggcccgtaac tacaccgaat cgttggagca cgcatcgcgc ggtaccgaac
     5821 tggtgctacc gaaggtacgc gtggatgcgt tgatgcggca tggtaacccc gcgctacgtt
     5881 tccgagtcag cgcgggctct gcttcggatc gagggcatcg cgcaggccgt cgccgaccag
     5941 gtagagcgcg agcgaggcgg agaagacgat gaaccccggg aagaaaacat tcgtccagtt
     6001 cgtccggtag aagcgatacg cctcgtccag catgttgccc cagctcggcg tcggcacctg
     6061 cacgccaacc ccgaggaagc tgagcgacgt ctcggcgatg atcagcgccg gcaccgtcag
     6121 cgacgccagc accagcatcg tcggcatcac gttcggaagg atgtacctga gcacgatccc
     6181 gactggcgag acgccgacca ctttcgccgc atcgacatac tcgcgcgatt tcagcgagag
     6241 cgcctcaccg cgcagcaacc gggagtcccc cggccacgag acgagcgcga ggatcagcgc
     6301 cagcatgtat gtcccgggcc ggtaaagcgt gctgatcagg atcaggagtg gcagcgtcgg
     6361 gatcgagagc agcacatcga ccagccgcat caggacaaaa tcaacccact tcccggcgta
     6421 gccggagatc aggccgacgc caaggccgat cgtcagctca aagaaggtcg ccagcaccgc
     6481 gacggtcagc gagatccgcg cgccgtacgc gagacgggtc aggatatccc ggccgttggc
     6541 atcgctgccg aggatgtagc cgttctcgcc cggcgccgag agcttggcgg cgaggtggtt
     6601 ctcctgcgga gtgaaaccag tcaggcgcga gacgacaccg gcgccgagcg cgaagatgac
     6661 cagcaggagc acgacgacca aagcggtcat cgcgacccga tcccgcgcaa aggtgccgag
     6721 cgcgcgccgg acgaggccct gcggtttccg cgttgcccgc tccgtctgcc tgacgttgat
     6781 atccgcgagc gtcgttgtgg tcgcgctcat gagacgcgca cccgcggatc gatcaggccg
     6841 tagaggatat cggccagcat gttgccgatc atcgtcagga aggcgaaaag cagcgtcgtc
     6901 gcctggacca tcgtgtagtc gcgccgcccg accgcctcga tcgtcaacga gcccatcccc
     6961 ggccaggcga agatgtactc gatcagcagc gcgccgccga acagccccgg aatcgacagc
     7021 ccgaccagcg tcgtcagggt caggagcgcg ttccgaaagg cgtgcccata gagcacccgc
     7081 aactcacgca cgcccttcga tctggccgtg cggatgtagt cctgcccgag cgcctcgagc
     7141 atcgatgagc ggatgtaggc ggtccagacg ccgatctgga agctggccag ggtgatcatt
     7201 ggcagaatca gatgctccag ccgatcggta gcgccgcctc cgccgcgcgg gctcttgatc
     7261 ccgcccgacg gcagggtcgg gaaccaccac agatcccatc tgttcgactg caccgagaag
     7321 aggatgatca gcaggatccc cagccagaag agaggaatcg aatagagcgc gaccgagaag
     7381 atgttgacca gccggtcgaa ggcccgcttg tgatagacgg ctgaatagat cccgaacgga
     7441 atcgagacgg ccagcgagaa gagcaacgcc gcactggtca gcagaagcgt gttcggcatg
     7501 gcggcccaga tccggtccgt gaccggcagc cggttgtaga acgacggccc gaagtcgagg
     7561 tggaagacga cgtcgcgcag ccactcgaca tagcgctgcg gccagggatc gttcaggccg
     7621 agctgctcgc ggatgcggtt gatatcttcc tggcggatct tcgggttgct gcgcgcggtc
     7681 atggcgaccg gcgaccccgg gatcaggttc atcagcgcga aggagatgaa cgagaccccg
     7741 aagagcaggg ggatcatgat caggattcgg cgaatgacga agcgcagcat ggttcagccc
     7801 gtagcgtaaa tggggcgagg gccgaagccc acgccccaag aggcctgatc gttattgctc
     7861 aatccagaga tactcgatgc cgggtccgcc ccacggaccg gggaacaggt tatgcatgcg
     7921 gttgttccag gcgacgatcg acaccgcgaa gttgatgatg cccaccggct gctcgtcatt
     7981 gacgatgttc gagtactgga tcatcagacg ggcgcgctct tcctggtcga ccgtgacctt
     8041 gatctgctcg ccaaggacat ctagctcggg gttgcagtac ttcatgtcgt tgaagccgac
     8101 ctggtactga tcgcacccga acatcgcgtc ctggatgaag gtcgagtccc agccgaaact
     8161 gtacaacgcc atctcgaagg tcggattcgt ggtcgtcgcc tcgatcagcg ccgggaactc
     8221 catcgccacc ggcttgatcc cgatccccac cgccttccag gcatcctgga agtaggcgac
     8281 catcgtgtcg gtggtggcgg atccggacgg atagaggaac tcgaagtcca tctcgatccc
     8341 gtccttgtcg acggttccgt tcccattggt gtcggtccag ccggcctcgg tcaggagctg
     8401 cttcgccttc tccggatcgt aggtgtagac ggtgttgatc tggtccgggg cataggcgta
     8461 gctgatcgac ggttgcgtgc cgttcgccac ttcgccgtag ccgagcagga tgttgtcgac
     8521 gatcgactgc cgatcaagcg catacatgaa ggcctgccgg acgcggacat cctggaagcg
     8581 ggtcgtcacc tccggatcga ggttgaactc gtagtaggtg aagccacgat acgggaagtc
     8641 ctcgaccgtg aactggggat cagccttgac cgtctcgacc tgctccggct cgagtccgtt
     8701 gtcgtcgagc tcgccggtca ggaacgcgtt cagctgggcc tcggtgtcgg cgaagatccg
     8761 caacacgatg tccttaatcc aggccgggcg tggagtgtac tgttcgttgc ggacgaggtg
     8821 aatctcctgg ccctgtgtcc agctctcgaa tttgaacggg ccggtgccca ccacgcggga
     8881 cggatcctgc ccggttgaac ccgggtccgt ggcccactga tcgcgcggaa tactctccca
     8941 gatgtgcttc gggatgatga tgaagccggc gttgttcagg aagtcagcgc ggaccccgtt
     9001 cgaaacgacc tgaatcgtgt cggcgtcgat gaccttccac gacgcgaccg catccaggaa
     9061 ctgcccggtg tatgggctgg ccagtaccgg atcggccagc gactcgaagc tgaaggcgac
     9121 atcatcggca gtaagatcga cgccgtcctg ccagaccttg tcggtgcgca ggtggaacgt
     9181 gtacgtcacg cagtcctcgg ccagctccca ataatcggtc acgccgcccg gaaccggttg
     9241 accggtatca ggatcgccac ccaggaagga gtcgaatacg aggccgacga cggccagcga
     9301 ggtctgctcc tcaacaatgt acggattgac cgactgtaga tcgccgaccg atccggtcac
     9361 gtaggtgcct tcggtgttgg tcggctgctc gatcgggaac tgctcttcga tctcctggcg
     9421 gatatcagcc gacgtcttcg acggtccggg ctcgcagctc gcggcgaccg ggctggcctc
     9481 cgggctcgcg atcgggctgg cgcccggtgt gctctccccg gtgaccggcg acgcctcagg
     9541 cgaggcatcc tgcgccgatg cgatcggcag gccgatcatg ctgaacaaca tgaatatggc
     9601 gagcactctg cccgcccagg tcaaccgcaa tcgcatccgc cacactcccc aaatacggaa
     9661 ttcaccaaac ccggaaacat ttgaccggcg aagcatgcct gatcgagccg cgccggggca
     9721 accgtttcgc gacatccgcg cattcccgaa aagtccagtt gactacttta gatgtgcatc
     9781 catacaatgc gcgcacccga acgaggcacg ttgcgacatc gtgccgcaac gacaatccag
     9841 ggtttagcgc acggcgatta cgccggggag gaccacctgt gggagagaac acgcaggtcc
     9901 gacagctcta ccgcgatgcg ttgagcggca agctcaaccg gcgggaagtc tttcagcgcg
     9961 gcattgcact tggtctgagc gcaaacgtca tcggcatgct ggcgatcaac gccgtcaaga
    10021 tgccgaccgc gttcgcggcg acagagggca agccgtcggc caccttctac gactggatgc
    10081 tcaacctcca cccccggatc acatccctgg gtgaggagat gggcgttgat atccagaccg
    10141 cgccgaccga gaacttcgga aacgaccgct tcatcgccga gggtctccag aagaacagca
    10201 cctgggactt ttacggcggt gtcacgccgt tcctggaaat gatcggcctg gttgacagcg
    10261 gcacgatcga gccgtggacc cagtacctgc cggccgggtt caccgatgac ttcgttccgg
    10321 cgaccctcac cgaaggaacc tacaacggcg agttctacgt ctggccgctc ctgctcgaca
    10381 tctgcgtgca gatgcgccat gccgggatcg tccaaaaggc tgggctcgat ccggaggcct
    10441 ccccggcgac gtgggacgag ttcatcgcga acggtcaaaa ggtcgtcgac agcggtgccg
    10501 ctccgtacgg catcgtcttc gaccatcgcg actggcgctc gctgattccg gttacccact
    10561 cgatcagcat cgacgtctat gacaaggaga ccggtctctt ccagtacgcc agcgatccgg
    10621 cgctcgaggc cctcgagatc ctcaagcgga tgatgccgct tgctccggcc gacctgctga
    10681 cggcagacgc cgtcgacaac acggtccatc cggacgaggc cgccttcgcc gctgagcagg
    10741 ttggctatta cttcaagtac cagaacgcgg gcttccagtt ttcggccaac tggccggatc
    10801 cgagccagct ccgcctcggc ggtctgccga agaccgagaa cggcgtcggc ggctccgtct
    10861 tctgggacac cggcgcagtg ctcttcacct acggcgcgaa caagccgaag gctgtggagt
    10921 acctgaccgc gttgtccaag gatcaggaga tgtggaagga cagcatcacc ggcaacgcgg
    10981 atcagggcac gatcccggtc ggtcagct
//
LOCUS       DATAOZ010000165         7595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7961022, whole genome shotgun sequence.
ACCESSION   DATAOZ010000165 DATAOZ010000000
VERSION     DATAOZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7961022"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..961
                     /locus_tag="VHR64_04500"
     CDS             296..961
                     /locus_tag="VHR64_04500"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEX3302118.1"
                     /translation="MKDSTRLNRRDLARFGIGALALGAVGVMPAQLLAAPKIESTQRG
                     EMPTTGGLRPGPLGLNPQPLTVAGFTPVSISIQKAGVDAIVETREIVDGVMQDPTGPW
                     VVSWYKETGRLGVPDENVVMAAHVDYWDVGPAVFYNIRVLAEGDEVDVTGDDGNVYRY
                     AVEWQKLFDADNAPIQDIVGPTDESALTLITCGGQFDYANGHYISRTVVRCKFSEKVA
                     STS"
     gene            1125..2450
                     /gene="thrC"
                     /locus_tag="VHR64_04505"
     CDS             1125..2450
                     /gene="thrC"
                     /locus_tag="VHR64_04505"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX3302119.1"
                     /translation="MSLIQDSSRAEPDNAFSNLTVRCMDCGHTFAATRPLTACPDCGG
                     LLDAVINTSWPIDRDTFGTALPPNLAQSGVWRYRALLPAIPDQYIVTRAEGRTPIYWD
                     DRIAQYAGVASMGLKHEGHNPTASFKDRGMTVGMSHAKAVGARFVACASTGNTSASLA
                     SYAASANIPALVLIPEGKISGGKLAQTLAHGALVVQIEGDFDAALALLRQVAADYDRY
                     QVYLVNSVNPFRPEGQKTTVFELLEQLNWEVPDVIALPGGNLGNTAAFGKALTELYET
                     RLIGRMPRIATIQAAGAAPFAAYYESGWDHFAPVQAETIATAIKIGNPASTARAMRAI
                     ISTGGVVTSVTDDEIMDAKAEIDRVGIGCEPASAASLAGVRKLVASGEIKPGETVVGI
                     LTGHVLKDTDAVTAYHLGPDSEYRPMANRPVSIPATLDALEKVLSRHAL"
     gene            2440..3342
                     /gene="thrB"
                     /locus_tag="VHR64_04510"
     CDS             2440..3342
                     /gene="thrB"
                     /locus_tag="VHR64_04510"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX3302120.1"
                     /translation="MRFSVRAPASSANLGPGFDALGLALDLWNSIEIETGGEGIRVRN
                     VGSDAPGMDGLENYSVRAMRQLAEEHGRELPPFHLTAQIDVPVARGLGSSAAAIVAGL
                     IAGNHLLELGLSNDQIFQAAARMEGHGDNVGAALHGGAILSVPALGRAVKLTDGADLD
                     LTTVVYIPDEHGATWVARAELPQHVALADAAFNVGAASGIVAGLITGDIGAIAAGMHD
                     RLHEPPRSRLYPHLIPMSEAARTAGAIGACLSGAGPTVLALVTHADVPAVCRAMQETG
                     RTAGIPGQCRHLPISPTGATLITE"
     gene            complement(3584..4033)
                     /locus_tag="VHR64_04515"
     CDS             complement(3584..4033)
                     /locus_tag="VHR64_04515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3302121.1"
                     /translation="MSKVVLVELSAENWEAVARMQVQPDQQNFVVDNLWTIAETQFYP
                     WAQRRVILAHGRIVGFAVYGMQPGDSELWLHRFMVAAEEQRSGIGRAALRLLIDEWKA
                     MPSLRMVKLSYEPDNAVAERLYASEGFVPGELADWGERIATLDLTTR"
     gene            complement(4102..4572)
                     /locus_tag="VHR64_04520"
     CDS             complement(4102..4572)
                     /locus_tag="VHR64_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3302122.1"
                     /translation="MSEEIHLVDLTVDNWEAVAALVVAPRQEAFVATNMKTIAETQFY
                     PSVRRRVIMAGDRPVGLAAYGIDTDDDNWWLFRFMIAGGEQGTGYGRRALRLLIDEWR
                     AIPECRFVLLGYKPENVVAERLYESMGFVPGEVASWGERIARLDLDRTSDPKGW"
     gene            complement(4569..5579)
                     /locus_tag="VHR64_04525"
     CDS             complement(4569..5579)
                     /locus_tag="VHR64_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX3302123.1"
                     /translation="MTAPQRIVTFGEAMVRLTPPFNERLERATSLNLTCGGAELNVAV
                     TAACLGHDATWVSALPDTPLGRVIMRAGRGHGVDMSRVSILPESAGRTGVYFLEEGTD
                     PRPSAVYYDRARSVMAQLESGAFDWPALLSSAAAFMVSGITPALGAGARAETMAAIGA
                     ANAARIPVFFDLNYRSKLWTEAEARACFIDLAPLVDVMFASRTGLETFFGITGDEHDD
                     VMLKACQRLGLAACVLTRKKGKQSRSQRMAATSALANGQVTATDWTEVEIVDRVGGGD
                     AFAGGFIVGYLEQPENPAFALELGLAASALKHTMAGDFLAATRAEVEAAMIAREGGAL
                     QR"
     gene            5815..6924
                     /gene="ugpC"
                     /locus_tag="VHR64_04530"
     CDS             5815..6924
                     /gene="ugpC"
                     /locus_tag="VHR64_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX3302124.1"
                     /translation="MARVIFDQVTKRYAGGVEAVKNLTMEIMDNEFLVLVGPSGCGKS
                     TAMRMVAGLEEITEGRIIIGDRVVNDLPPKDRNVAMVFQSYALYPHMSVRDNLAYPLK
                     LAKVPKDERERRVRETAQMLDIDQYLDRKPKALSGGQRQRVALGRAIVRNADLFLMDE
                     PLSNLDAKLRVQTRAELIKLHERVQTTTIYVTHDQVEAMTMGDRIAVMSLGLLQQLDT
                     PQNLYDKPSNKFVAGFIGSPSMNFVDTTIEQQGGKIYAKAAGFTLRVPDAKVNAVQGY
                     LGQAITLGIRPEHLLEIHQADASIPEDSLIHATVDVVELLGNEIFVYLTTSAGQVITA
                     RMDPDLHLQRGQQIQVTAPPDRLHFFDPQSEIAIG"
     gene            7112..>7595
                     /locus_tag="VHR64_04535"
     CDS             7112..>7595
                     /locus_tag="VHR64_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302125.1"
                     /translation="MGRRVFFSLALLVSTFAGLLSPLAAGAAQRAASGLSDNQYVDPT
                     YSFGVTWDADLYEAEELLDSNDDPYGVSLSGDALFAYAFAGEYTSLRACVRDEADAIG
                     GLDSVTSFDEATDLDLPKTDPDARAALYRLEYEDSDSGDVSTFIQYIECRELIQDEEP
                     L"
BASE COUNT         1369 a         2538 c         2396 g         1292 t
ORIGIN      
        1 ttccgaacaa gaaccatgcg cgagccgtgg gtccacctcg atcagatcgc ggaggaccag
       61 ctcgcgcggt tcattgccgg ccggccggga aatacgtgat cccaccgatg atgcatatga
      121 cgagccggtc gatgatgaaa tcagcaaatc ggacgcgccg gggttgtccg agtgatacga
      181 tttcagcgct cgatgcgtta gaggatgcag actgtcaacg cgcgagccga tctccccgac
      241 gcccagcgac ggaccgcgcg gaacacccgc ttcggcaacc ttcagggagg cactgatgaa
      301 ggactcgact cgacttaatc gccgcgatct ggcccgattc ggcatcggag ccctggccct
      361 cggcgcggtc ggcgtcatgc cggcccagtt gctggctgcc ccgaagatcg aatccaccca
      421 gcgcggcgag atgccgacga cgggtggcct ccgcccaggc ccgctcgggc tcaatccgca
      481 gccgttaacg gtcgccgggt tcactccggt gtcgatctcg atccaaaagg ccggcgttga
      541 tgccattgtc gagacacgcg agatcgtcga cggcgtgatg caggacccga ccggtccatg
      601 ggtcgtttcc tggtacaagg agaccggtcg cctcggcgtg ccggacgaga acgtggtgat
      661 ggccgcgcac gtcgactact gggacgtcgg tccggccgtc ttctataaca tccgggtcct
      721 ggccgaaggc gatgaagtcg atgtcaccgg cgacgacggc aacgtctacc gatacgccgt
      781 cgagtggcag aagctgttcg acgccgacaa tgcgccgatc caggacatcg tcggcccgac
      841 cgacgagagc gccctgacgc tgatcacctg cggcggccaa ttcgactacg cgaatggcca
      901 ctacatctct cggacggtcg tccgctgcaa gttctccgag aaggtcgcct cgaccagtta
      961 gccgcgcaag ctcaagcaag cgaaacagac cggcaagcag cacgcttgcc ggtcttgtcg
     1021 tccgggaccg ggagcgctga tctgcgttat gctgcgcgaa cggtttatcc gctcgcttgc
     1081 ccatcgatca tcgactgatt tcggatggct ttttgggagg ggctgtgagc ctgatccagg
     1141 attcttcgcg cgccgaaccg gacaacgcgt tctccaatct gactgtgcgt tgcatggact
     1201 gcggccacac cttcgccgcc acccggccat tgaccgcctg ccccgactgc ggcgggttac
     1261 tcgatgccgt catcaacacc tcgtggccga tcgatcgcga taccttcgga acagccctgc
     1321 caccgaatct cgcacagtcc ggtgtctggc gataccgcgc gctgctcccg gccatccccg
     1381 accagtacat cgtgacgcgc gccgaggggc ggacaccgat ctactgggac gaccggatcg
     1441 cgcagtacgc cggggtcgcc tcgatgggcc tcaagcacga ggggcacaac ccgaccgcgt
     1501 cgttcaagga tcgcggcatg accgtcggga tgagccacgc caaggccgtc ggcgcgcgct
     1561 tcgtcgcctg tgcctcgacc ggcaacacgt cggcgtcgct tgcctcgtat gcagcctcgg
     1621 ccaacatccc ggcgctggtc ctgattcccg agggcaagat ctcgggcggc aagctggccc
     1681 aaacgctcgc ccacggcgcg ctggtggtcc agatcgaagg tgacttcgac gcggcgctgg
     1741 cgttgctgcg tcaggttgcg gccgattacg accggtacca ggtctacttg gtcaactcgg
     1801 tcaacccatt ccggcccgag ggccagaaga cgaccgtgtt cgagctgctc gagcagctca
     1861 actgggaggt cccggacgtg attgccctgc ctggcggcaa cctgggcaat acggccgcgt
     1921 tcggcaaggc gctgaccgag ctctacgaga cgagactgat cggccggatg cctcgcatcg
     1981 cgacgatcca ggcggcgggc gcggcgccgt tcgccgccta ctacgagtcc ggctgggatc
     2041 actttgctcc ggttcaggcc gagacgatcg ccaccgcgat caagatcggc aacccggcca
     2101 gcacggcgcg agccatgcgg gcgatcatct cgaccggcgg cgtggtcaca tcagtcacgg
     2161 acgacgagat catggacgcc aaggcggaga tcgaccgggt cgggatcggc tgcgagccgg
     2221 cctcggccgc gagcctggcc ggtgtccgca agctggtcgc gtccggcgag atcaagccgg
     2281 gcgagacggt ggtcggcatt ttgaccgggc acgtcctcaa ggacacggat gcggtcacgg
     2341 cgtaccacct cggcccggac tccgaatatc gaccgatggc gaaccggccg gtctcgatcc
     2401 cggcgacact cgatgcgctc gagaaggtgc tgagccggca tgcgctttag cgtccgcgct
     2461 ccagcctcat ccgcaaatct cggccccggc ttcgatgccc tgggactcgc gctcgacctc
     2521 tggaactcga ttgagatcga gaccggcggt gagggcatcc gcgtccgtaa tgtcgggtcg
     2581 gacgcgcccg gcatggacgg cctcgaaaac tactcggttc gcgccatgcg ccagcttgcc
     2641 gaagagcacg gacgcgagct gccaccgttc catttgacgg cacaaatcga cgtcccggtc
     2701 gcgcgcgggc tcggcagctc ggccgcggcg atcgtggccg ggttgatcgc cgggaaccac
     2761 ttgctcgaac tcgggctcag caatgatcaa atcttccagg cggctgcgcg catggagggt
     2821 cacggcgaca acgtcggcgc ggccctgcat ggcggggcga tcctctctgt cccggcgctc
     2881 ggccgcgcgg taaagctgac cgatggcgcc gacctcgatc tgacgaccgt ggtgtatatc
     2941 ccggacgaac acggcgcgac ctgggttgcc cgggccgagc ttccgcagca tgtcgcgctg
     3001 gcggatgcgg cgttcaacgt cggcgcggcg agcgggatcg tggccgggct gatcacgggc
     3061 gatatcgggg cgatcgcggc cggcatgcat gaccgcctgc acgagccgcc acgctcccgc
     3121 ctttacccgc acctgatccc aatgtcggag gcggcgcgga ccgccggcgc gatcggcgcc
     3181 tgcctgagcg gcgctggtcc gaccgtgctc gccctcgtca cgcacgccga cgtgcccgcc
     3241 gtctgccgcg cgatgcagga aaccggccgc accgccggta tccccggcca gtgccgccac
     3301 ctccccattt cgccaaccgg cgcgacccta atcaccgagt gaagtcaacc caggaagagc
     3361 agataaatat tatgtagcgc ggggatgccc tctctcctgg aggagagagc cggagagagg
     3421 aacatgcccc gcccagaccc aacgaaatcc acatcggtgg gggaagcaac cccgtcggtg
     3481 gtttggttct ataaagggcg cgtggggatc aacgatacag ggcgtggtaa ccccgtgctc
     3541 cgtctttggg ttgacggcca cccaactgat tcaagcgaga tagtcagcgg gtcgtgagat
     3601 cgagggtggc gatgcgttcg ccccagtcgg cgagctcgcc gggcacgaag ccttcgctgg
     3661 cgtagaggcg ttccgcgacg gcgttgtccg gctcgtagct gagcttgacc atccgcaggc
     3721 tcggcatcgc cttccactca tcgatcagca accgcaacgc cgcacgacca atgccggacc
     3781 gctgctcctc ggcggccacc atgaagcggt gcagccacag ttccgagtcg cccggctgca
     3841 tgccgtagac cgcgaacccg acgatccggc cgtgggcaag aatcaccctc cgctgcgccc
     3901 acgggtagaa ctgggtctcg gcaatggtcc agaggttgtc gacgacgaaa ttctgctggt
     3961 ccggctggac ctgcatccgg gccacggctt cccagttttc agcagacagc tcgaccagta
     4021 cgactttgct catgagtggc ggtactcctc gtccaacccg atcatgatgg cgcgccggcg
     4081 atgcgcgtgc agtgatcggc gttaccaacc cttgggatcg gacgttcgat caaggtcaag
     4141 ccgcgcgatc cgctcgcccc acgatgcgac ctcgccgggg acgaatccca tactctcgta
     4201 gagccgctcg gccacgacat tctccggctt atagccgagc agcacgaacc ggcactcggg
     4261 gatcgcccgc cactcgtcga tgagtaaccg caaggcccgc cggccgtacc ccgtgccttg
     4321 ctcgccgccg gcgatcatga atcggaacag ccaccagttg tcgtcgtccg tatcgatccc
     4381 gtacgccgcg agtccgaccg gccgatcacc cgccatgatc acgcggcgac gcactgaggg
     4441 gtagaactgc gtctcggcga tggtcttcat attggtggcg acgaacgctt cttgtcttgg
     4501 cgcgacgacc agcgcggcca cagcctccca gttgtccacc gtcagatcga cgaggtggat
     4561 ctcttcgctc accgctggag cgctccgcct tcgcgggcga tcatcgccgc ctcgacctct
     4621 gcgcgcgtcg cggccaggaa atcgccggcc atagtgtgct tgagcgcgct cgccgccagg
     4681 ccgagctcga gcgcgaaggc gggattctcc ggctgctcca gatagccaac gatgaaacca
     4741 ccggcaaagg cgtcgccgcc gccgacccga tcgacgatct cgacctcggt ccagtctgtc
     4801 gcggtcacct gaccattggc cagcgcgctg gtcgccgcca tgcgctgcga gcggctttgc
     4861 ttgcctttct tgcgggtcag cacgcaggcc gcaagcccaa gtcgctggca cgccttcaac
     4921 atcacgtcat catgctcatc gccggtgatc ccgaagaagg tttccaggcc ggtacgggag
     4981 gcaaacatga catcgacgag cggcgccagg tcaatgaagc aggcccgcgc ctcggcctcg
     5041 gtccagagct tgctccgata gttgagatcg aagaagaccg ggatccgggc tgcattggcc
     5101 gccccgattg cggccatcgt ctcggcgcgc gcaccggcgc cgagcgcggg cgtgatacct
     5161 gacaccatga atgccgccgc actggagagc agcgccggcc agtcgaacgc accggattcg
     5221 agctgcgcca tgaccgatcg cgcccggtcg taatagacag cggacggccg tggatcggtc
     5281 ccctcttcca gaaagtacac cccggttctt ccagccgact ccggcaggat cgagacgcgc
     5341 gacatgtcca cgccgtgccc gcgcccggcc cgcatgatca ccctgccgag cggcgtgtca
     5401 ggaagcgccg aaacccaggt cgcatcgtgc cccaggcagg ccgccgtcac cgccacattg
     5461 agctccgcgc cgccacaggt caggttcagc gaggtcgcgc gctcgagtcg ctcattgaag
     5521 ggcggggtca gccggaccat ggcttcgccg aatgtcacga tccgttgcgg ggcagtcacg
     5581 tgtgtttctc catggcaggc gcgaatcgaa tcattttagg gattgtagtc ggcgcgcacc
     5641 cggggtagac cctcaactgc ttgacagtgc ccagatatgc cccggatact gcgcccacac
     5701 gagttgtaag gcgccgcaac cgggttcgcc cgtatcgcag gtgatattgg ctgaagatga
     5761 gggcgtctct taactgccgt cgggcagttc tgtaatccaa gggaggacgc ggcgatggcg
     5821 agagtcatct ttgaccaggt caccaagcgt tatgcaggcg gtgtcgaagc ggtcaagaac
     5881 ctgaccatgg agatcatgga caacgagttc cttgtgctcg ttggtccgtc gggctgcggc
     5941 aagtcgaccg ccatgcgaat ggtggccggc ctggaggaga tcaccgaagg ccggatcatc
     6001 atcggcgatc gggtcgtcaa tgacctcccg ccgaaggacc gcaatgtcgc gatggtgttc
     6061 cagagctacg cgctgtatcc gcacatgtcc gtccgcgaca acctcgcata tccgctcaag
     6121 ctggcgaagg tgccgaagga tgagcgcgag cgccgggtcc gcgagaccgc gcaaatgctc
     6181 gacatcgatc agtatcttga tcgcaagccg aaggcgcttt ccggcggtca gcggcagcgc
     6241 gtcgcgctcg gccgcgcgat tgtccgaaat gccgatctct tcctgatgga cgagccgctc
     6301 tcgaacctcg acgccaagct gcgggtccag acgcgagccg agctgatcaa gctccacgag
     6361 cgcgtccaga cgaccacgat ctacgtcacg cacgaccagg tagaggccat gacgatgggc
     6421 gaccgaattg cggtcatgtc gctcggcctt ctgcagcagc tcgatacgcc gcagaacctg
     6481 tacgacaagc catcgaacaa gttcgtggcc gggttcatcg gctcgccgtc gatgaacttc
     6541 gtcgatacca cgatcgagca gcaaggcggc aagatctacg ccaaggccgc cgggttcacc
     6601 ctgcgtgtgc ccgacgccaa ggtcaacgcc gtccagggct acctgggcca agcgatcacg
     6661 ctcggcatcc ggccggagca ccttctggag atccaccagg cggatgcaag catccccgag
     6721 gattcactga tccacgccac agtcgacgtc gtcgagctgt tgggaaatga aatcttcgtc
     6781 tacctgacga ccagcgccgg ccaggtgatt acggcccgca tggatccgga tctgcacctg
     6841 cagcgcggac agcagatcca ggtcaccgcg ccgccggatc ggctccactt cttcgatccg
     6901 cagtccgaaa tcgcgatcgg gtaaccgccg ggatcgcagc tttgcacgta cgtgggtcag
     6961 ggttcgttcc ctggcccaca ttcgtatcca gcagacatcg ggaacaatcc aatcgtcccg
     7021 cgcgttttcc aaccgtggct cgtttgacag acggccgcga ggtgctagca tcgcaccacg
     7081 tgcaaaacaa tcaggaaggg cttggggttc tatgggtcgt cgcgtcttct tctcgcttgc
     7141 gctgctcgtc tcgacctttg ccggtctact gtcgccactt gccgccggcg ccgcacagcg
     7201 agcggcgagc ggtctttccg acaatcagta cgtcgatccg acctactcgt tcggggtgac
     7261 ctgggatgcg gatctctacg aggcagaaga gctactggac tcgaacgatg atccgtacgg
     7321 cgtctccctg agcggggatg cgttgttcgc gtacgccttt gccggcgagt acacctcgct
     7381 gcgtgcctgc gtccgggacg aggccgacgc catcggcggc cttgactctg tgacgagctt
     7441 cgatgaggca acggacctcg acttgcccaa gaccgacccg gatgcgcggg ccgcgctcta
     7501 ccggctcgag tacgaggatt ccgattccgg cgatgtcagc acctttatcc agtacatcga
     7561 atgccgcgag ctcatccagg acgaggagcc gctgg
//
LOCUS       DATAOZ010000166         6711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3225062, whole genome shotgun sequence.
ACCESSION   DATAOZ010000166 DATAOZ010000000
VERSION     DATAOZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3225062"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..637)
                     /locus_tag="VHR64_04540"
     CDS             complement(191..637)
                     /locus_tag="VHR64_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3302126.1"
                     /translation="MIVFYVLLAALGGALVVVQASINGELRLLVGDPYRTALISTTVS
                     TIFLFALSGLMNGRPVPDGSTFSNASWWMWTGGILGAVYVAAAAVLITKLGSAVLFTL
                     VIFGQLVTAVLMDHFGLIGLEKHPVSAARIVGILLVLAGVVIVRKS"
     gene            complement(730..1128)
                     /locus_tag="VHR64_04545"
     CDS             complement(730..1128)
                     /locus_tag="VHR64_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX3302127.1"
                     /translation="MPNTAIPESHRDLLAARPAVTLATIGPDGFPQVTAVWFLVEDDG
                     TVRMSLNTARQKVKNLRRHPEASLFFTDPDNPYRTLEIRARVALEPDSDYAFADRVGA
                     KYSADMRKNDRPGESRVVVTFTPTKVNTYG"
     gene            complement(1218..2132)
                     /locus_tag="VHR64_04550"
     CDS             complement(1218..2132)
                     /locus_tag="VHR64_04550"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX3302128.1"
                     /translation="MYDDDTIAAVRPRMFSIAYRMLGSVSEAEDVVQEAFLRQQRALA
                     DGVEIQSPAAYLSTVVTRLAIDELKSARAQRETYVGEWLPEPLLTDQRAVDPAAKAEQ
                     ADSLSWAFLLLLERLSPVERAVFLLHDVFGYGFDEIAEIVGKSEANCRQLASRARRHV
                     EEGKPRFEASRRKRNELAGRFFSAMSDGNLDGLVKLLAADVAVYGDGGGKVPAWTRPI
                     TGVDHVGRLLIGMGRQIREYGARIEQHEVNGQPGAMILDAEGELVSVLSLEIVDDTVR
                     RVRGVINPDKLHHLGPVADVWALLQQGP"
     gene            complement(2148..3125)
                     /locus_tag="VHR64_04555"
     CDS             complement(2148..3125)
                     /locus_tag="VHR64_04555"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3302129.1"
                     /translation="MRVFLAGGTGAIGRRLVPQLVARGHEVTATTRDPDKAEILFTLG
                     ATPVLMDGLDGASVGEAIAKAQPEAIIHEMTALAGTPDFKHFDRWFAQTNRLRTEGTE
                     HLLAAAKASGVRRFVAQSYTGWTNTRSGSLIKTEADPFNPDPAPVQRATLAAIQFVEE
                     AVTSAPLDGIVLRYGNLYGPGASEAMVEIVRGHKMPIVGNGAGVWSWLHVDDAAAATV
                     AAVEGTQCGIFNIVDDEPAAVSEWLPFLAEALGARQPMHVPAWLAKFVIGDGGVQLMT
                     VGRGSSNEKAKRELGWRPIWSSWREGFRHGLIDSPRAMPVDIHRHLTAA"
     gene            3370..3621
                     /locus_tag="VHR64_04560"
     CDS             3370..3621
                     /locus_tag="VHR64_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302130.1"
                     /translation="MQDDEAAPYHDRCDFHIADWQDRETGTMDTVINASDYAHRSSVS
                     VAIPAPTEDHLAFFQALWFWIPAGLGLWAALIWALMHLI"
     gene            complement(3966..5570)
                     /locus_tag="VHR64_04565"
     CDS             complement(3966..5570)
                     /locus_tag="VHR64_04565"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3302131.1"
                     /translation="MITLREIIESCFPSGSGRQVGDPLLDREVTWATRPRPSPPAFGH
                     LSGGELVLLRLDELTNVDDRLTLDGAVRQLAGFNVSAVAVAGPVAETHRSLASARKVA
                     LLELPPTTDLGEIERRATHLIAERRRDRQRRGHEAGRRLMELAIAGEPLATIISTLNQ
                     LSRQAVALEGRDGRLMTYHARPGQPTREEIAPLLVATGDAVRNWLAITSDASPAEPPT
                     MHLALGPELDRTIAPVIGRDGLLGCLSLIGPAGAWSGDDALLASRGAAACAIVLAREL
                     ASVAARREIELNVLDEVLDGALRNETTLLQQARRLGHDLENAHRMMVIDLDDDDPESL
                     RAAITSALDAVVGRSLWRMAGESIEVVLSPNADPGMIVRMLREHLGLPGIAAGAGLTH
                     PGLAGLQRSRHEARRALTIQRRLGIGSDVACFSELGAYQLLFAAEHLPEFAAFHDETL
                     SKLVAYDEANRSGLVETLRAWFDANSSPKEAARRMGIHRNTMLYRLERVRTLTGYDLD
                     DADTRFRLQLALCMNTIMSSRIVATT"
     gene            complement(5577..>6711)
                     /locus_tag="VHR64_04570"
     CDS             complement(5577..>6711)
                     /locus_tag="VHR64_04570"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX3302132.1"
                     /translation="KVELDLFLMPDPETLAIFPFEADGQRRARLCCSVVRRDAQPFAG
                     DPRSVLERNLAQARELGYDYRVAVEMEYYLLPGDGTLPDRESGAGYFSVADDQIATTR
                     DAVLTSLQGMGITVGGSHHETGPGQEELDLPDVGALRMADQLITVRQVIRSVARRHGL
                     RATFMPKPMEDAPGSGMHVFQSLNRTVDGRDALGTEGNNLTQEAHWLIGGQIEHAIGM
                     TLITNPTVNSYKRLNAGHRAPRYGTWARVSQASLIRVPNWIDTDQAEIELRSPDAMAN
                     PYLAFSVALAAGMDGIRRRTDPGDPFDESFVAFDDAEFMRRGVSRLPSTLGEAIPAFA
                     DDVVVTQAIGGYISDQLIAVKRDEWEAYRAHVSPWELQRYVDA"
BASE COUNT         1178 a         2381 c         2016 g         1136 t
ORIGIN      
        1 tgcaaggtgt gccagtcggc ttcagccgcc ttaggctggg tcagtcccgg ccttctgccc
       61 ggggcggcac ggaacggccg tttgaatatc aaccggacgc aaacagcccc ttcagtgggc
      121 ttttcatctc tcagcccggg catttattcc cgggcgattc gcgcgatccg acttcaggtt
      181 gagatttggt ttacgacttc cgcacgatca cgacacccgc cagcaccagg agtatcccga
      241 cgatccgggc agcggagacc gggtgcttct ccagcccgat cagcccgaag tggtccatca
      301 atacagccgt caccagctgc ccgaagatga ccagcgtgaa cagcaccgcg ctgccaagct
      361 tggtgatcag cacggccgcc gccgcaacgt acaccgctcc caggatcccg ccggtccaca
      421 tccaccacga agcgttcgaa aacgtcgagc catccggcac cggtcggccg ttcatcaggc
      481 ccgagagcgc gaagagaaaa atcgtcgaca cagtcgtcga gatcagcgcg gtccggtacg
      541 gatcgccgac cagcagccgc agctcgccgt tgatgctcgc ctgcacgacg acgagcgcac
      601 cgcccaacgc agccagcagc acgtagaaca caatcatcgc ccgcaccccg cccccttcac
      661 cgcaatccgg ccagcgccaa cgtcgcgcat ggtgccagat ttcgggtctg caagtggagg
      721 ccgggagggc tagccgtagg tattgacctt ggtcggagtg aatgtgacga ccacgcgcga
      781 ctcgcccgga cggtcgttct tccgcatatc cgcgctgtac ttggcgccca cccggtcggc
      841 gaaggcatag tcggagtccg gctccaacgc tacccgtgcc cggatctcca gtgtccggta
      901 cgggttgtcc ggatccgtga agaacaggct cgcctccggg tgccgccgca gattcttcac
      961 cttctgccgc gccgtattca gcgacatccg cactgtcccg tcatcctcga cgaggaacca
     1021 gaccgcggtc acctgtggaa acccgtccgg cccgatcgtc gccagggtga ccgccggacg
     1081 ggccgccagc agatcgcggt gagattcggg aatagcggta ttcggcatca attcgacgtc
     1141 ctctcgcgtg actcaggcgt atgagaaatg cgcctgactg agcgtaggtc ggattgggcg
     1201 gctcgaccct gccgtaacta cggcccctgc tgcagtagcg cccacacgtc ggcgaccggg
     1261 ccaaggtggt gcagtttgtc cgggttgatc accccccgga cccgccgcac tgtgtcgtcg
     1321 acaatctcga gcgaaagcac gctgaccagc tcgccctcgg cgtccaggat catcgcccct
     1381 ggctgaccat tgacctcgtg ctgctcgatc cgcgctccat actcgcggat ctgccggccc
     1441 atcccgatca gcaaccgacc aacatgatcc acgccggtga tcggccgggt ccaggccggt
     1501 acttttccgc cgccatcgcc atagaccgca acatcggccg ccaacagctt gaccagaccg
     1561 tcgagattgc cgtcggacat cgccgaaaag aatcgccctg ccagctcatt ccgcttccgg
     1621 cgtgacgcct cgaagcgcgg cttgccttcc tcgacatgcc gccgtgcgcg ggaagcaagc
     1681 tgccggcaat ttgcctcgct cttgccaacg atctccgcga tctcgtcgaa cccatacccg
     1741 aagacgtcgt ggagcaggaa gacggctcgc tcgaccgggc tcagccgctc gagcaggagc
     1801 agaaacgccc atgagagcga atctgcctgc tctgccttcg cagccgggtc cacggcgcgc
     1861 tgatcggtca gcaggggctc cgggagccac tcgccgacat acgtctcgcg ctgggcccgc
     1921 gccgatttca gctcgtcgat cgccagccgc gtgaccaccg tcgagaggta ggcggccggc
     1981 gactggatct cgacaccatc ggccaatgcc cgctgctgcc gcaggaacgc ctcctgcacc
     2041 acatcctctg cctcgctcac cgagccaagc atccggtagg caatcgagaa catccgtggc
     2101 cgcaccgcgg cgatggtgtc gtcgtcgtac atgactctat ccacacctca ggcggccgtc
     2161 aggtgccgat ggatgtccac tggcatggcg cgtggcgaat cgatcagtcc gtgccggaag
     2221 ccctcacgcc aactgctcca gatcgggcgc cagccgagct cgcgctttgc cttctcgttc
     2281 gacgagcccc tccccacggt catgagctgc acgcccccgt ccccgatcac gaatttcgcg
     2341 agccacgccg gaacgtgcat cggctgccgg gccccaagtg cctcagcgag gaacggcagc
     2401 cactcggaca cggccgccgg ctcgtcgtcg acgatgttga atatcccgca ctgagtcccc
     2461 tcgacggccg ccacagtcgc tgccgccgca tcatcgacat ggagccacga ccagaccccc
     2521 gcgccgttgc cgacgatcgg catcttgtgc ccgcggacga tctcgaccat ggcctccgat
     2581 gcgcctggtc cgtacaaatt gccgtaccgc aggacgatcc cgtcgagcgg tgccgatgtc
     2641 accgcctcct cgacgaattg aatcgccgcc agcgtcgccc gctgcaccgg cgccgggtct
     2701 ggattgaatg gatctgcctc ggtcttgatc agactgcccg agcgggtatt ggtccagccg
     2761 gtatagcttt gggccacgaa ccggcgcacg cccgacgcct ttgccgccgc cagcaggtgt
     2821 tcggtcccct cggtgcgcag ccggttcgtc tgggcgaacc aacggtcgaa gtgcttgaaa
     2881 tccggcgttc cggcgagtgc cgtcatctcg tggatgatcg cctctggctg tgccttggca
     2941 atcgcttcgc caaccgaagc accgtcgaga ccatccatca acacgggtgt cgccccgagc
     3001 gtaaacagga tctctgcctt gtccggatcc cgggtcgtcg cggtcacctc atgaccgcgc
     3061 gcaaccagtt gcggcaccaa tcgccgcccg atcgccccag ttcctcctgc cagaaagacg
     3121 cgcatatccg actcctttcg tctctcatgc gtctactggc aatcaagacg agacgggatg
     3181 ccggaatgtg acatgcaggg tcgcgatggc ttgccagatc gcgcaataaa atgttacatc
     3241 cgggctacaa agatagcgcg cgcgctcgcg acatcattgt tcgttccatg acctgcgtac
     3301 cgaacggcct gggtcaaaag cctgcacctg tctgggaaag gtacggacat gcggggggac
     3361 gactggggaa tgcaagacga cgaagccgct ccctaccatg atcggtgtga cttccacatc
     3421 gcagactggc aggacaggga gaccggcacc atggacaccg tgatcaacgc atccgattac
     3481 gcgcatcgct ccagcgtgtc cgtcgccatc ccggccccga ccgaagacca cctcgccttt
     3541 ttccaggccc tctggttctg gatcccggcc ggcctcggcc tctgggccgc cctcatctgg
     3601 gccctgatgc acctcatcta gctcccgaaa gaccgcaaaa tttgatcagg tctgcccccg
     3661 gcaaacgtag cgcggggatg ctctctaccc ttgaggggag agtcggagag aggaacacgc
     3721 cccgcttggt gcatccccac gcacgcgtat tgatgccact gagttatcgg ggtttgtttc
     3781 cttaacgcca tgaccctccg cggatccggg cacggcatgg caaccccacg ctacgtcaaa
     3841 acattaggcg tagcgcggcc cgcctctggg ccgccttgtg acgcatgtcc ccctggagga
     3901 cgcaggacgg ctccatgaac ctcacgtgtc gttaacacca ctatgccggc cacaggggat
     3961 cggtgctacg tggtggccac gatgcgcgaa ctcatgatcg tgttcatgca gagcgcgagc
     4021 tgcaatcgga atcgggtgtc ggcgtcgtcg aggtcgtagc cggtcagggt ccggactcgc
     4081 tccagccgat agagcattgt gttgcgatga atccccatcc gccgcgcggc ctccttcgga
     4141 ctgctgttgg cgtcgaacca ggctcgcaat gtctcgacca gacccgatcg attcgcctcg
     4201 tcatacgcca cgagcttgct cagcgtctcg tcatggaacg cggcgaactc cggcaggtgc
     4261 tccgccgcaa agagcagctg gtacgcgccg agctcgctga agcaggccac gtccgaccct
     4321 atccccagcc gccgttgaat ggtcagggcc cgccgcgcct cgtgccgtga ccgttgcagc
     4381 cccgccaggc ccggatgcgt caaccccgca ccggccgcaa tgcccggcaa ccccagatgc
     4441 tcacgcagca tccgcacgat cattccggga tccgcgttcg gcgacagcac gacctcgatc
     4501 gactcgccgg ccatccgcca gagggaccgg ccaacgacgg catcgagcgc ggacgtgatc
     4561 gccgcccgca gcgattcggg gtcgtcatca tcgagatcga tcaccatcat tcggtgtgca
     4621 ttttcgagat cgtggccgag ccgtcgggcc tgctgaagca gcgtcgtctc gttccgcagc
     4681 gcgccatcca gcacttcgtc gaggacgttc agctcgatct cgcgccgggc cgcgaccgag
     4741 gccagctccc gcgccagcac gatcgcgcag gccgccgcgc cacgtgaggc cagcaatgcg
     4801 tcgtcgcccg accacgcgcc cgccggtcca atcagcgaca ggcacccgag cagcccgtcc
     4861 cgcccgatca ccggtgcgat cgtccggtcc agctccggac ccagcgcgag gtgcatggtc
     4921 ggcggctcgg ccgggctggc atcgctcgtg atcgccagcc agttccggac cgcgtctccc
     4981 gtcgcgacca gcagcggcgc gatctcctcc cgtgttggct ggccgggtcg cgcgtggtag
     5041 gtcatcagcc gaccatctcg cccctcgagt gcgaccgcct ggcgcgagag ctggttgagc
     5101 gtactgatga tggtcgccag cggctcgccc gcgatcgcca actccatcaa ccgccgcccg
     5161 gcctcgtgcc cgcgtcgttg ccgatcccgt cgccgctccg cgataagatg cgtggcccgg
     5221 cgctcgattt cgccgagatc ggtcgtgggc ggcagttcca ggagcgcgac cttgcgagcg
     5281 ctcgccagcg agcggtgcgt ctcggcgacc ggcccggcca cggcgaccgc cgagacgttg
     5341 aaaccggcca gttgccggac cgcgccgtcg agggtcaatc ggtcgtcgac gttggtcagc
     5401 tcatcgagcc ggagcagcac cagctcgccg ccgctgagat ggccgaatgc tggcgggctc
     5461 ggtcgcggcc gcgtcgccca ggtcacttcg cggtcgagca ggggatcccc gacttgccga
     5521 ccggagccag acggaaagca gctctcgata atctcgcgca aggtgatcat ggcgcgtcac
     5581 gcatcgacat aacgctgcag ttcccacggg ctgacatggg cgcgatacgc ttcccactcg
     5641 tcccgcttga ccgcaatcaa ctggtcggag atgtagccgc caatcgcctg cgtcacgacc
     5701 acgtcgtcgg caaaggccgg aatcgcctcg ccgagggtcg acggcaaccg gctcacgccg
     5761 cgccgcatga actccgcgtc gtcgaacgcc acgaagctct catcgaatgg gtcgcctgga
     5821 tcggtccgtc tccggattcc atccatcccg gctgccagcg cgaccgagaa ggcgagatag
     5881 gggttcgcca tcgcatcggg cgagcgcagc tcgatctctg cctggtcagt atcgatccag
     5941 ttcgggaccc ggatcagcga tgcctggctg actcgcgccc aggtcccata tcgcggcgcc
     6001 cggtgacccg cattcagccg cttgtaggaa ttgacggtcg ggttcgtgat cagcgtcatc
     6061 ccgatcgcgt gctcaatctg gccgccgatc agccagtgcg cttcctgggt caggttattg
     6121 ccctcggtcc cgagcgcatc gcgaccatca acggtgcgat tgaggctctg gaagacatgc
     6181 atcccggaac ccggcgcgtc ctccatcggc ttcggcatga aggtagcccg cagaccatga
     6241 cgccgcgcga cggagcgaat cacctgtcga accgtgatca attggtcggc catccgcagc
     6301 gcaccgacgt ccggcaggtc gagttcttcc tggccgggcc ccgtctcgtg gtgcgagccg
     6361 ccgacggtga tgcccatgcc ttgcagcgag gtcagaacgg cgtcgcgggt cgtcgcaatc
     6421 tgatcgtccg cgacgctgaa atagcccgca cccgactccc gatccggcag cgtgccgtcg
     6481 ccggggagca ggtaatactc catctcgacc gcgacccggt agtcgtaacc gagctcccgc
     6541 gcctgagcca aattgcgctc gagcaccgag cggggatcgc cggcaaacgg ctgtgcatcc
     6601 cgccgcacga ccgagcagca gagccgcgcc cgccgttgtc catccgcttc gaacgggaag
     6661 atcgccagcg tctcgggatc gggcatcagg aagagatcaa gctcgacctt g
//
LOCUS       DATAOZ010000167         7090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1384755, whole genome shotgun sequence.
ACCESSION   DATAOZ010000167 DATAOZ010000000
VERSION     DATAOZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384755"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VHR64_04575"
     CDS             <1..421
                     /locus_tag="VHR64_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX3302133.1"
                     /translation="TEGIRQLTEILRPLGVQVLVYDLPHWNGAGECLHLMSMISPVAE
                     KVAVVYSRMMAVAFVEWLKVAGWSLIEMPESEFDTMGCNVLALGEGKVVIANRNPETS
                     ALLQAAGLTVLEYQGDHISHNRQGGPTCLTRPVYRAS"
     gene            500..1663
                     /locus_tag="VHR64_04580"
     CDS             500..1663
                     /locus_tag="VHR64_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3302134.1"
                     /translation="MKRNAASATQYSTDDLLKLEPSTVMGLLERGRITRRDVFKAISA
                     AGVATFGLQMLDDSALAKEELKMIIWEGYADDKYRVPFEEANDCTVSYTEAGTGDEMF
                     AQMKDSDGKNYDMVSASSDLPQRLYDADLLAEIDTSKLTNYNDLWDQFKTPNYITFDS
                     KLYGVNFAWGPTLILSNPDDVTTAPTSWNALLDEQHKGKISTWNYPLQIAQYSLLLNP
                     APEDPYVLSDDQLAQIKDLLIKQRPLIRKYWDTGLELQQLFLNKEVVIADGWTWITNQ
                     LKAQGGKVVETVPTEGVTGWSDSWVISKAAKNYDLALKWADWMIGPEGQVGLTEVMGY
                     SITNKKAAATLPQDVQEQLRLTNVEDEYAKIKMWKFVDNYDKWVQVWNEATQG"
     gene            1740..2630
                     /locus_tag="VHR64_04585"
     CDS             1740..2630
                     /locus_tag="VHR64_04585"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302135.1"
                     /translation="MATRVYRTSAPGRGRSRWFGLAGLLTAPVGWMVIFYLIPIGILL
                     VHAFWSVKYPEIDHTLTVKNFRTILSSELYPRVLVRTVVMAAGVTLADIVLAFPLAFW
                     LAKRVRRNRDLLLFLVIFPLWSSYLIRIFAWKTLLGTNGILNSFLQWTGILHQPLSFF
                     LYSKWAIFIALTHVWLPFMILPLFTVLDRIPDPLLEASSDLGAGWFATFRTVILPLCK
                     PGLLAGGLATFSLTMGDYIAPTLLGGPGDQMIGKIVADQFGVADNWPLGAALIIPVLI
                     MISLLLWVSNQAGATERTAV"
     gene            2627..3439
                     /locus_tag="VHR64_04590"
     CDS             2627..3439
                     /locus_tag="VHR64_04590"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302136.1"
                     /translation="MSAVQATRGRRFSLGGASLTVAAICVFIFLYIPLAVIVLYSFSG
                     EVVNVWPIGSYTTHWYGEMWRDSQIRDGILLSFRIGLIAATIAIVLGTLTAIAVDRFD
                     FFGKSLLRFCVVLPITLPGIVTGVAMLSFFSVMGMQLSNRTIIIGHTTFCITLILNNV
                     VARLSQIPRSLDEASADLGATPWRTFWRVTFPLILPAILSGGILAFTLSFDEIVVTLF
                     LKGGDATLPLVIWARLRRGFSPEINAAATMIIGVSFVAVLLANRVSKWTGSS"
     gene            3440..4534
                     /locus_tag="VHR64_04595"
     CDS             3440..4534
                     /locus_tag="VHR64_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302137.1"
                     /translation="MSMHVEGQGARIELVNLEKHFEGVVAVRNTNLTVQPGEFLTLLG
                     PSGCGKTTTLRMIGGFEYPTGGSILIDGKDVASLPPFRRPINTVFQQYALFPHMTVAR
                     NVGYGLEMSGIGKAERERRVADALAMVRLANVEKRRPSELSGGQQQRVALARALVNRP
                     KALLLDEPLGALDLKLRKAMQLELKELNRETGATFIYVTHDQEEALTMSDRIAVMNEG
                     RILQLGTPAEIYEHPVDRFVADFIGQSNLIPGTLEAREGDRCMVRIPTGGLIEARLRD
                     NPSVGASVVVSIRPERVELREQPAESLDGSFPATISEIVYLGSHNQIVLYATPNMRLI
                     VNVPTDQLARPGDSVVATVPSRYATCFIAE"
     gene            4620..5549
                     /locus_tag="VHR64_04600"
     CDS             4620..5549
                     /locus_tag="VHR64_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX3302138.1"
                     /translation="MDKSARFRSVDCLTIIEQRLPALTGASRQVAEAIMRDPWAVLGM
                     TIYDVAEMSNVSLPSVTRFCRAVGFGGFRELVQGIAQSLGRIDSKDFESLHGSQPHDG
                     GIKALAESIVSRQVEALQTTLHALDYEAIERATQLIADARRVVILGHGAAQVPALGLS
                     VKLMWGGVAAFEAAPDMFSNIVITSQPGDVVIGVSHQGRTRDTIELLRLARKFGATTI
                     GITTVPNSPLAEVSDVSLAVLSPDVARAGTFLIAFDALMVVADILAATVSDRKWKGDP
                     PNRSDVVEWIERNLRVGPSTFVDNRHARRVPNG"
     gene            5554..6807
                     /locus_tag="VHR64_04605"
     CDS             5554..6807
                     /locus_tag="VHR64_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3302139.1"
                     /translation="MTGSIEAARSKAAKFARANQDNVIALTEELVAAPSENLPGDETR
                     PAEVVQSWLPRLGLPEAVTIASEPHRPNLLITIDSGRPGPHLGICGHLDTKPVGDAAA
                     EWKTDPFTATTIGDRLYGLGTTDMKGAVAAMLVAGAAFAQVKHEVGGSLTLILTADEE
                     YGSVYGAEYLTRQNALDVDGIVLGEPSGVREDWEAIRVVSRGISCFKVRVFGTQIHSS
                     ISDQLSVINAVEQMARLYCGFKERFRPRYPVHPLCGSGPTLNIGVKTLGGVGFGVNAG
                     MAEFWSDVRTTPGMDQELFQQDVSVALEAAAADIPGIRYEVEYPPNLTWIEPTEVAAD
                     HPLTLACLDAGRRVLGREIPLAAFPGASDAYPFQYIGGIPTIASFGPGWLTPAHGPNE
                     WISLKSLREAAEIYSHLAISYCSNS"
BASE COUNT         1277 a         2185 c         2244 g         1384 t
ORIGIN      
        1 caccgagggc atccggcagt tgaccgaaat cctgcgcccg cttggcgtgc aggtgctggt
       61 ctatgacctg ccgcactgga atggcgcggg cgagtgcctg cacctgatgt cgatgatcag
      121 cccggtagcc gagaaggttg cggtcgtcta ctcgcggatg atggcggttg cgtttgtcga
      181 atggctcaaa gtggccggct ggtccctgat cgagatgccg gaatccgagt tcgacaccat
      241 gggctgcaac gtcctcgcac tcggcgaagg taaggttgtg atcgccaatc gaaaccccga
      301 aacctcggct ttgctgcagg ctgcggggtt gaccgtcctg gaataccagg gtgaccacat
      361 ctcgcacaac cggcagggcg gaccaacctg cctgacgcga ccggtttacc gcgccagttg
      421 atatcgtagc cgcgcatacg atattgtgcg gcctgccagc actagggcaa atcgaagact
      481 catcggcagg ggaatgagca tgaagcgcaa cgctgcatca gcgacccaat actcgaccga
      541 cgaccttctg aaacttgagc cttctaccgt gatgggcttg ctcgagcggg gccggatcac
      601 gcggcgcgat gtcttcaagg ccataagcgc ggccggcgtg gcgaccttcg gcctgcagat
      661 gctggatgac agcgcgctgg ccaaggaaga gctcaagatg atcatctggg agggctatgc
      721 ggacgacaaa taccgcgttc ccttcgaaga agcgaacgac tgcacggtca gctataccga
      781 agccggcacc ggcgacgaga tgttcgccca gatgaaggac agcgacggca agaactacga
      841 catggtctcg gcttcgtctg acctgccgca gcggctttac gacgccgacc tgctggccga
      901 gatcgacacc agcaagctga ccaactacaa cgatctctgg gaccaattca agactccgaa
      961 ttacatcacg ttcgacagca agctctacgg cgtgaacttc gcctggggcc caaccctcat
     1021 cctctcgaac ccagatgatg tgacaacggc gccgacatcc tggaacgcgc tgttggacga
     1081 gcagcacaag ggcaagatct cgacctggaa ctaccctctc caaattgccc agtactcgtt
     1141 gctcctgaat cctgcgccgg aagatccgta tgtgctgagc gacgaccaac tcgcccagat
     1201 caaggacttg ctgatcaaac aacgcccatt gatccgcaag tactgggaca ccggcctcga
     1261 gctccagcaa ctgttcctga acaaggaggt cgtgattgcc gacgggtgga cctggatcac
     1321 gaaccagttg aaggcgcagg gcggcaaggt tgtcgagacc gtcccgaccg agggcgtcac
     1381 gggctggtcg gactcgtggg tgatctccaa ggcggccaag aactacgacc tcgcgctcaa
     1441 gtgggcggac tggatgatcg gtcccgaggg ccaggtgggt ctgaccgaag tgatgggcta
     1501 ctcgatcacg aacaagaagg cggctgccac tttgccgcag gacgtgcagg agcagctccg
     1561 gctcaccaac gtcgaggacg agtacgccaa gatcaagatg tggaagttcg tcgataacta
     1621 cgacaagtgg gttcaggtct ggaacgaggc cacgcaaggc taagttcacc tgatgatcag
     1681 gctgcggccg gcatccaatt ccaggggccg gccgttttgc cacagaccgt gagaacctga
     1741 tggcgacccg ggtgtatcgg acgagcgcgc ctggccgggg acgatcccgg tggtttgggc
     1801 ttgccggctt gctgaccgcg ccggttggct ggatggtgat cttctatctg atcccgatcg
     1861 ggattctgct ggttcacgct ttctggtcgg tcaagtaccc cgagatcgat cacacgctca
     1921 ccgtcaagaa tttccggacc attctctcga gtgagttgta tccccgggtt ttagtgcgca
     1981 cggtggtcat ggcagcgggc gtcaccctgg cggacatcgt gctcgccttc ccgctggcct
     2041 tctggcttgc aaagcgggtg cgtcgcaatc gcgatctgct gctcttcctg gtcatcttcc
     2101 cgctctggag ctcgtacctg atccggatct tcgcctggaa gacgttgctg ggcacgaacg
     2161 gaatcctgaa ctcgttcctg cagtggacag ggatcctgca tcagccgctc tcgttcttcc
     2221 tctattcgaa gtgggcgatc ttcatcgcct taacgcacgt ctggctgccg ttcatgatcc
     2281 tgccgctctt taccgtgctg gatcgcatcc cggacccgct gctcgaggca tcgtcagatc
     2341 tcggggcagg ctggtttgcc acctttcgga cggtgatcct gccgctctgc aagccggggc
     2401 tgctcgcggg tggactggca acattcagcc tgacgatggg cgattacatc gcgccgacac
     2461 tgctcggcgg tccgggtgac cagatgatcg gcaagatcgt agccgaccag ttcggcgtgg
     2521 cggacaactg gccgctcggc gccgcgttga tcattcccgt gctgatcatg atctcgctcc
     2581 tgctctgggt atcgaaccag gccggagcga cggagcgaac ggccgtatga gcgcggtcca
     2641 ggccaccaga ggtcgtcggt tctcacttgg cggcgcgtcg ctgaccgtgg ccgcgatctg
     2701 cgtcttcatc tttctttaca tcccgctggc ggtcatcgtc ctgtactcgt tcagcggcga
     2761 ggtggtcaat gtctggccaa tcggctccta cacgacgcat tggtacggcg agatgtggcg
     2821 cgattcccag attcgcgatg ggattctgct ctcgttccgt atcggactga tcgcggcgac
     2881 aatcgcgatt gtccttggga cgctgaccgc gatcgccgtg gatcgattcg actttttcgg
     2941 aaagtcgctc ctccgcttct gcgtggtctt gccgatcact ttgccgggaa tcgtgaccgg
     3001 cgtggctatg ctctcgttct tttcggtcat gggcatgcag ctatcgaacc ggacgatcat
     3061 tattggccac accacattct gcatcacgct gatcctgaat aatgttgtgg cgcgcctctc
     3121 gcagattccg aggtcactgg acgaggcttc ggccgatctc ggcgcgacgc catggcggac
     3181 cttctggcgg gtgacctttc cactgatcct gccggcgatc ctctcgggcg ggatcctggc
     3241 atttaccttg tcgttcgacg agatcgtcgt cacgctgttc ctgaagggag gggatgcgac
     3301 acttccactc gtgatctggg cgcggctgcg gcggggattt tccccggaga tcaatgccgc
     3361 ggcaaccatg atcatcggcg tctcgttcgt cgctgtcctg cttgcaaatc gcgtctcgaa
     3421 atggaccgga tcatcctgaa tgagcatgca cgtcgaaggg cagggcgccc ggattgagct
     3481 cgtcaacctg gagaagcact tcgaaggtgt tgtcgcggtc cgcaacacga atctgacggt
     3541 ccagcctggc gaattcctga cgttgctcgg cccctcgggc tgcggcaaaa cgacgacgct
     3601 gcgcatgatc ggcggcttcg agtacccgac tggtggatcg attttgatcg acgggaagga
     3661 cgtcgcgtcg ctgccgccct ttcgccgtcc gatcaacacg gtcttccagc agtacgcgct
     3721 cttcccgcac atgacggtgg ctcgcaatgt cggctacggt ctcgagatgt ccggcatcgg
     3781 caaggcagag cgggagcgac gagttgcgga cgcgctggcg atggttcggc tcgccaacgt
     3841 cgagaagcgc cggccgagcg aactctccgg cggacagcag caacgcgtgg ccctggcgcg
     3901 ggcactggtc aaccggccca aggcgttgct cctggacgag ccgctgggag cgctcgacct
     3961 gaagctgcgc aaggccatgc agctcgaact caaggagctg aatcgcgaga cgggcgcgac
     4021 cttcatctat gtcacacacg accaggaaga ggcgctgacg atgtcggacc ggatcgcggt
     4081 catgaacgaa ggacgcatcc ttcaactcgg tacaccggcc gagatctacg agcatcccgt
     4141 cgatcggttc gtggccgatt tcatcggaca atccaacctg attcccggca cgctcgaagc
     4201 gcgagagggc gatcgctgta tggtgcggat cccgaccggc ggcctcatcg aagcgcgcct
     4261 ccgcgacaat ccgtccgtcg gcgcgtcggt ggtggtgtcg atccggccgg agcgcgtcga
     4321 gctgagggag caacccgcgg agtcgctgga cggatcgttc ccggcgacga tcagcgagat
     4381 tgtctacttg gggagccaca atcagatcgt gctctatgcg acgccgaaca tgcggttgat
     4441 tgtcaacgtg ccgactgatc agctcgcccg tccgggcgat tcggtcgttg cgaccgtgcc
     4501 atcccggtac gccacgtgct tcattgccga ataagggcgc ttgacggtct gaaaatcagc
     4561 cgtagaatac gcgaaagttt ctttcttgcc atgtaactgg ttacccggtg gggtgcagga
     4621 tggacaagtc cgcccgtttt cgctctgtcg actgcctgac catcatcgag caacgattgc
     4681 ccgcgctgac tggcgcgtcg cgccaggtgg ccgaggccat catgcgcgat ccgtgggccg
     4741 tgctgggcat gacgatttac gacgtggccg agatgagcaa tgtcagcctg ccctcggtca
     4801 cccggttttg ccgggcggtc ggttttggtg gattccggga gttggtgcag ggtattgcgc
     4861 agtcgctcgg gcggatcgac tccaaggatt tcgagtcgct ccacggctcg cagccgcatg
     4921 acggcggcat caaggcgctg gccgagtcga tcgtcagccg gcaggttgag gcgctccaga
     4981 cgactctcca cgctctcgac tacgaggcca tcgaacgcgc cacgcagctg atcgcggacg
     5041 cacggcgggt tgtgattctc ggtcacggcg cggcccaggt tcccgcgctc ggtctctcgg
     5101 tcaagctgat gtggggcgga gtcgcggcct tcgaggcggc gcccgacatg ttctcgaaca
     5161 tcgtgatcac gtcgcagccc ggcgatgtcg tgatcggcgt ctcgcaccag ggtcggaccc
     5221 gcgacaccat cgagctcctg cggctggcac gcaagttcgg ggcgaccacg atcgggatca
     5281 cgaccgtgcc caactcgccg ctcgccgaag tcagcgacgt ttcgctggcc gtgctctcgc
     5341 ccgatgtcgc ccgggctggg acattcctga tcgcgttcga tgcgctgatg gtcgtagccg
     5401 atatcctcgc cgcgaccgtt tcggatcgga agtggaaggg cgacccaccg aaccggagcg
     5461 acgtcgtcga gtggatcgag cggaatctca gggtaggtcc ttcgaccttc gtggacaacc
     5521 ggcacgcccg gcgcgtgccg aacggataga ggcatgacag gatcaatcga ggcagcgcgg
     5581 tcgaaggccg cgaagtttgc gcgcgcgaat caggacaacg tgatcgcact gactgaggag
     5641 ttggtcgcgg cccccagtga gaacctgccg ggtgatgaga cccgtcccgc tgaggtggtc
     5701 cagagttggc tgccgcgcct cggcttgccg gaggcagtta cgatcgcttc cgagccgcac
     5761 cgaccgaacc tgctgatcac gatcgactcc ggccggccgg gtccgcacct cggcatctgc
     5821 ggtcacctcg acaccaagcc ggtcggcgat gccgccgccg agtggaagac agaccccttc
     5881 accgcgacca cgattggtga ccggctgtac ggcctcggca cgacagacat gaaaggggcg
     5941 gtcgcggcga tgctcgtggc cggcgcggcg ttcgcgcaag tcaagcacga ggtcggcggc
     6001 tcgttgacgc tgatcctgac ggccgatgag gagtacggca gcgtttacgg cgccgagtac
     6061 ctgacccgcc agaacgcgct cgatgtagac ggaatcgtgc tcggcgaacc gtcaggcgtg
     6121 cgcgaggatt gggaggcgat tcgcgtcgtc tcgcgcggga tatcctgctt caaggtccgc
     6181 gtgttcggga ctcagatcca ctcgtcgatc tcggaccagc tttcggtcat caacgcggtc
     6241 gagcagatgg cgcgactgta ttgcgggttc aaggagcgct tccggccgcg ctacccggtt
     6301 catccgctct gcgggagcgg tccgaccctg aacatcggcg tcaagacgct cggcggcgtc
     6361 ggattcggcg tcaatgcggg catggcggag ttctggtcgg atgtgcggac cactccgggg
     6421 atggaccagg agttgttcca gcaggatgtc tccgtcgcgc tcgaggcagc cgcggcggat
     6481 atcccgggaa tccggtacga ggttgagtac ccgccaaacc tgacctggat cgaaccgacc
     6541 gaggtggcgg ctgatcaccc gttgactctc gcttgtctcg atgccggacg gcgcgtgctc
     6601 ggccgggaga ttccacttgc ggccttcccg ggcgcatcgg acgcctaccc gttccagtac
     6661 atcggcggta tcccgaccat cgcctcattc ggtcccggct ggctcacccc cgcacacggt
     6721 cccaacgagt ggatctcgct caagagtctc cgcgaagccg ccgaaatcta ctcccacctc
     6781 gcgatcagct actgttccaa ttcgtagtgg cggggcctcg tacccgtctc gagccgcccg
     6841 acacgatagt tcgcatgcat taactggctc caggtggata cggcaaccgg tccggctcca
     6901 tgaggcattc gattgcgcca ttccaggcgg gtacggagcc ccgccactac ggacggttta
     6961 accggaatct tcggctgctc caggcgggta cggagcttcg ccatcaccga aatcaattgt
     7021 tggcaacgtt tctgattacg gcgaggatgt cctcgccgta ctggttggcc ttgatgacgc
     7081 cgatgcccgg
//
LOCUS       DATAOZ010000168         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2437479, whole genome shotgun sequence.
ACCESSION   DATAOZ010000168 DATAOZ010000000
VERSION     DATAOZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437479"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VHR64_04610"
     CDS             <1..489
                     /locus_tag="VHR64_04610"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA methyl transferase PRC-barrel
                     domain-containing protein"
                     /protein_id="HEX3302140.1"
                     /translation="DVTQPGEIVSEQGDVIGGHDGLVHHTVGQRRGIRIAGPKPLYVL
                     ELKTDRNQLVVGDRERAVARRIGADGVSFTDGAWPEAEFAADAVVRYRGVPVPCAVRP
                     GATEGEVEVVLRGGGTAELPIASPGQAIVFYRGDEVLGGGTIRHVEREAELSGRTGEV
                     AE"
     gene            486..884
                     /locus_tag="VHR64_04615"
     CDS             486..884
                     /locus_tag="VHR64_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302141.1"
                     /translation="MSKAKWWAIGAGVAFVVLTAVAIWALYQAGGEDQSALERLRDIS
                     IIFIVALSLITVLLLAAITAALGYLTFQVKDRMIPLLEELTGTARRIRGTTEFMSEEA
                     VKPFLAVAGTYAKMRGTMKSFVSRGNGRKD"
     gene            complement(1197..1391)
                     /locus_tag="VHR64_04620"
     CDS             complement(1197..1391)
                     /locus_tag="VHR64_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302142.1"
                     /translation="MPIALIVIGALLVVGSVFADSLGFTWGGEGYGWKQMLATITGLV
                     IALGGVGLLTRPQPRSRKRT"
     gene            1906..>3136
                     /gene="coxB"
                     /locus_tag="VHR64_04625"
     CDS             1906..>3136
                     /gene="coxB"
                     /locus_tag="VHR64_04625"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX3302143.1"
                     /translation="MLLTMALMGAMVLLPSVAAAADDVYSTISPESKDGRDIQFLYKL
                     IFWLALFVFVGVQIAIVYSVMRYRRRAEDEPRPEQIHGNQKLEVIWTIIPAIVLVAIF
                     IPTVRTMYRIDDRANEGTYTIEVYGKQWWWEVHYTQPDTVAGVVTANEFYIPVNTRVR
                     IELMSSNVIHSFYVPQLAGKMDVMPGHKNVMSVEADKPGMYYGECAEYCGESHAYMRF
                     KIMAVPQDQFDLFISGWKQGASSQAASLAPDGNVDNVPTALGLCLGCHRIGGVPNNPS
                     GQPLPDPPMGIAGGTQPNQIMGPNLSMLACRTTLAAGIVPNDDQHLREWLHNPGGVKQ
                     GNFMASMIHEGTLTNDQIDQIVAYLKTLHPEQGCPVITGVNADKVHRLADENAPAQTA
                     DESTTSDAAFNVEARNKS"
BASE COUNT          582 a          984 c         1005 g          565 t
ORIGIN      
        1 gatgtcacgc agcccggcga gatcgtcagc gagcaaggcg acgtgatcgg cgggcacgat
       61 gggctggttc accatacggt cgggcagcgc cggggaatcc ggatcgccgg gccgaagccg
      121 ctctacgtgc tcgaactgaa gactgaccgc aatcagctcg tcgtcggcga tcgcgagcgg
      181 gccgttgccc gccggatcgg ggccgacggc gtctcattca ccgatggcgc ctggcccgag
      241 gcggagttcg cggccgacgc cgtcgtccgc taccggggcg taccggtgcc ctgcgcggtg
      301 cgcccgggcg ccaccgaagg cgaggtcgaa gtcgtgttgc ggggaggagg gacggcagaa
      361 ttgccgattg cgtcaccggg acaggcgatt gtgttctatc gcggcgacga agttctcgga
      421 ggcggtacga ttcgtcacgt cgagcgagag gccgaactca gcggccgcac aggtgaggta
      481 gctgaatgag caaggcgaaa tggtgggcga tcggtgcggg tgtggccttc gtcgtgctta
      541 ccgctgtggc gatctgggcg ctgtaccagg ccggcggtga ggatcaatcc gcgctcgagc
      601 ggctccggga catctccatc atcttcatcg tcgcgctttc cctgattacg gtcctgctcc
      661 tggcggccat tactgccgcg ctgggctacc tgaccttcca ggtcaaggat cggatgattc
      721 cgctgctcga ggagctgacc ggcaccgcgc ggcgaatccg gggcacgacc gagttcatga
      781 gcgaagaggc cgttaagccg ttcctggcgg tcgcgggcac ctacgccaag atgcggggca
      841 ccatgaagtc cttcgtctcc cgcggcaacg gccgcaagga ctgacctcga ccgcaagacc
      901 aggtaatgcg tcgcgcgtcg ggaccgcatt ccgctcttgt tgcccaagat ggggaggtag
      961 tacggggtta ccacgccccg cccgggcccg acggtggccg ctcgtcgggg aaaccaaccc
     1021 cgtcgattgt tcgacaccat cgaccagacg tagtgatcac cgattcgggg cgtggtgacc
     1081 ccgcgctatg ttaggtgtag tcaacaagca actcgccacg cgcatagaac gtaaaaccca
     1141 gtgggttgaa cccgccttct ggtcctgagt tgcggatggt ttcaaacccc ggcgtgctac
     1201 gtccgcttgc gggaccttgg ctgtggccgg gtcagcaaac cgacaccgcc gagcgcgatc
     1261 accaggccgg taatggtcgc cagcatctgc ttccagccgt agccctcgcc gccccacgta
     1321 aacccgagcg agtccgcaaa aacggagccc accaccagga gcgcaccgat cacgatcagc
     1381 gcgatcggca tccaccgata tccattttgc cgggtcggct gagattccat tccagtcctg
     1441 aacgattggc tgatttgcac atccggcgct tgtagcgccg tgcgtgcgca cgcatccctt
     1501 cccttcgatg gtagtccagt ttgcggactt tcgcactcat gcccaaggct ggggtaggaa
     1561 tccttgacca ggagcgatcg caatcgtgta cgatgcgcgc catgaggcaa gtgccgagcg
     1621 cggttcaccg cgccctggcg cgcctctggc actcgttggg gagcggggcc agtcgcggag
     1681 ttcacggatc ttcctgatct cggtagcgat tgggaattga tatgcacggc aggacacgaa
     1741 ttcgcgtgac tcttgcgccg ttcccacagc ggttcggcga cgaggagtgc gcgttttttg
     1801 ttggctgagc gaggtgcgag ggattcggtg cgagcaggag gcagcatgag gggtcgggag
     1861 cggcacagag cacccattca gaagtcggcc cggaacaggc ttgtcgtgtt gttgacgatg
     1921 gcgctgatgg gagccatggt gctcctgcca agcgtcgccg ccgctgccga tgacgtctac
     1981 tcgacgatct cgccggagtc caaggacggc cgcgacatcc agttcctgta caagctgatt
     2041 ttctggctgg cgctcttcgt ctttgttggc gtgcagattg ccatcgtcta ttccgttatg
     2101 cgctaccggc gccgtgctga ggacgaaccc cgcccggagc agatccacgg caaccagaag
     2161 ctggaagtga tctggacgat catcccggcc atcgtgctgg tcgccatctt cattccgacc
     2221 gtccgcacga tgtaccgtat cgacgaccgc gcgaacgaag gcacctacac catcgaggtt
     2281 tacggcaagc agtggtggtg ggaagtccac tacacgcagc ccgacaccgt ggccggcgtg
     2341 gtcaccgcga acgagttcta catcccggtc aacacccgcg tccgtatcga gctgatgtct
     2401 tccaacgtca tccactcctt ctatgtgccg cagcttgcgg gcaagatgga cgtgatgccc
     2461 ggccataaga acgtgatgtc ggtggaggcg gacaaacccg gcatgtacta cggcgaatgc
     2521 gccgagtatt gcggcgaatc ccacgcctac atgcgcttca agatcatggc ggttccgcag
     2581 gaccagttcg atctattcat tagcggctgg aaacagggcg ccagctcgca ggcggcgtca
     2641 ctggcgccgg atggcaatgt cgacaatgtg ccgacggccc tcggtctctg cctcggctgc
     2701 caccgaatcg gcggcgtgcc caacaatccg agtggtcagc cgctgccgga tccgcctatg
     2761 gggatcgccg gcgggaccca gccgaaccag atcatgggcc cgaacctgag catgcttgcc
     2821 tgccgcacca cgctggctgc cggcatcgtt ccgaacgacg atcagcactt gcgcgagtgg
     2881 ctccataatc cgggcggagt caagcagggc aactttatgg cctcgatgat ccacgaaggc
     2941 acgctcacca acgatcagat cgaccagatc gtggcctacc tgaagacact ccatccggag
     3001 caaggctgcc cggtcatcac cggtgtcaat gcggacaagg tccaccgcct ggcagatgag
     3061 aacgcgcccg cccagaccgc cgacgagtct acgacgtccg acgcggcatt caacgtcgag
     3121 gcgcggaaca agtcat
//
LOCUS       DATAOZ010000169         7431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4807458, whole genome shotgun sequence.
ACCESSION   DATAOZ010000169 DATAOZ010000000
VERSION     DATAOZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4807458"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..836)
                     /locus_tag="VHR64_04630"
     CDS             complement(21..836)
                     /locus_tag="VHR64_04630"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX3302144.1"
                     /translation="MQSKITTLSEAVGRIPDGGRLALGGNTIHRGPGAAVHEIARQGK
                     RGLDVIKTAGSYDIDLLCATGCASSVSAGYVGYETVLGLAPSYRAAVESGTVVASEHA
                     CYTVISGLRAAIQGVPFMPVAGLFGSDLLRTRDFRTVADPYTGEEVVAIRAIRPDVAI
                     IHVQEADECGNARIVGTLFEDVLMAQAADVVILTAERIVDGSAFETDPSLATISSLYV
                     DAVVDAPRGAWPFGCAGEYEPDLAYLTAFVQASRDRDALQTFIRERVLDPVPA"
     gene            920..2527
                     /locus_tag="VHR64_04635"
     CDS             920..2527
                     /locus_tag="VHR64_04635"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX3302145.1"
                     /translation="MYPPEVYKSYLTPFRFLQRSAAVFREKVAIIHRDQCITYPELNA
                     RVNRLASALRNAGIAREDRVAVLLPNIPQMLEAHFGVPLSGGIMVAINTRLKTDEIAY
                     ILDHSGAKALIVDSELAPLVEPAFSELLALETVITVHDVDAGIHLEGPDYEAFLASGS
                     PEELDWPIDDEDQVISINYTSGTTGKPKGVMYTHRGAYLNSLGELIETRMSSTSTYLW
                     TLPMFHCNGWCYPWAVTGIGGTHVCLRKFEPGEAWDLIRKHNVTHLCGAPTVLISMAN
                     HPAAPTEPLPRQLIVSVAAAPPSPSIIAQMESLGIEIVHVYGLTEVYGPYTVCEWQEQ
                     WDERPVEERSRIKARQGVGYLVADDARVVDDNMVDVPADAQTLGEVIMRGNNTMKGYF
                     RDDEATDRAFRGGWFHSGDLGVMHPDGYIELRDRAKDIIISGGENISTIEVEQAIAAH
                     PAVLEVAVIGVPDDYWGETPKAFVVLRDGASATEQEIIDHCKSRLAGFKCPRAVHFGE
                     LPKTSTGKIQKFVLREREWAGRSSRIN"
     gene            2548..2742
                     /locus_tag="VHR64_04640"
     CDS             2548..2742
                     /locus_tag="VHR64_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302146.1"
                     /translation="MRHINIVSYLILLTGLIMTVAGAALDLGAVVTLIGMMLVVAGIV
                     KIITVRIWNGFFDSDTVAGE"
     gene            2750..3091
                     /locus_tag="VHR64_04645"
     CDS             2750..3091
                     /locus_tag="VHR64_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302147.1"
                     /translation="MSEQREEKQPPGYRGTTGRLGSTNRTPAEQARLQKSRQNRQIFS
                     GAEIRPPSRALSSYPAQSTTLTQPQLLYAASLGILLVLGLLLWLIFGMGVASIIFFLL
                     ALGLLGGWLAF"
     gene            3092..3448
                     /locus_tag="VHR64_04650"
     CDS             3092..3448
                     /locus_tag="VHR64_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3302148.1"
                     /translation="MAAISWSDIPATEVYPGISRQILNGDRQTMVRYVYQPGSIFPEH
                     HHPEEQVTVVISGRIEFTVAGEAEVLASGQAAVIPPDVPHGARVIGDEPVETFTSMSP
                     RREQHPAPNPETRNER"
     gene            3445..4305
                     /locus_tag="VHR64_04655"
     CDS             3445..4305
                     /locus_tag="VHR64_04655"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX3302149.1"
                     /translation="MTKKESGRGHLRVVGKSARQTQSGPQTYRPGQFGTMFDLTGKGA
                     LVIGGGGGIGGAIAHALADFGARVAIADLSLDAAQAIATSCQRDGAGTTTCRSIDVTN
                     PDHVNQVVAEIEAELGKIDILVNSAGINIRKDVTDYTPEEWFKIINTNLSGVFFVTQA
                     AARGMLARGYGRVISIGSVSSLMGHPQHAPYAATKGGVAIMTKAMATEWAMKGVTVNA
                     IGPAYTVTGLITELMNNPVKYREIIETIPMGRLGNPEDIVGAAVYLASDAASFVTGQT
                     IYVDGGRTAD"
     gene            complement(4620..6149)
                     /locus_tag="VHR64_04660"
     CDS             complement(4620..6149)
                     /locus_tag="VHR64_04660"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX3302150.1"
                     /translation="MPVVDVQNAEQPLVLALDIGTSSTRAIIFDRRGHALRDSECQLK
                     YELTTTPDGGAEVDARMLADLVGKSIDAVLERHKVPIAAVGVAAFWHSLMGVDADGEP
                     TTPVYLWADTRSREDVDAIKQEFDAHELWQRTGCFVHSSYWPGKLRWLARTQPDLVRT
                     TRTWCSFTGYLLRKLFGADGISVSMASSTAMMNATTHQWDDLAIAASGVDPDRLAPIR
                     HEREPLTGMAPEFSRRWPALAEAPWFRGIGDGACANVGSGGLGPNRIALTVGTSGAMR
                     MVRRLPAGSSPNTPETLWTYRLDDRAAVIGAAISNGGKVVDWIGDLTGSAFGSEAFRC
                     AEQLEPDSHGLTILPFLAGERAPIWSDWATGAVSGLNLATTAADLIRAGMESVSYRLG
                     MIYADLATQAEPDHQILANGGAILRSPVWMQMIADVTQHSVVALPPEEESTARGAALM
                     ALEYAGLIKSLFDADDPAGYGQTIEPDPGRAEVYRAAMERQNRMLDLLYQDGKPLLGN
                     P"
     gene            6280..7155
                     /locus_tag="VHR64_04665"
     CDS             6280..7155
                     /locus_tag="VHR64_04665"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HEX3302151.1"
                     /translation="MNEHSFDQLARNLAHQTDRRTILGSVIAAGAALIGIRHAAFATP
                     QSQGGLVFDYYQAIDRRAFKTAYGYLGAKFRARQTLQNFTTGFSDTVYDDLVITNVRP
                     DAMRNQVVYDVTVTAWHLDGSVHRFTGTYTEGQEAGATKLIDAVIREVPVHDMPPLCA
                     ASDLEATMTGDAGAGQRYGTVTATNVAAGSCVLGGIPRVVVTDAAGNPVISARPEPNV
                     PITTVTLAPGKSATLGIHWSNWCGTPVSGEPQVSVMLRGGEGRITNLTGLAVPPCLSE
                     PGGASTLNVKPWQIG"
     gene            7244..>7431
                     /locus_tag="VHR64_04670"
     CDS             7244..>7431
                     /locus_tag="VHR64_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX3302152.1"
                     /translation="MLSDAVRDFLNERRFAVLATSNPNGTIQQSVMWYLLEDNTIVMN
                     TAKGRIKFRNMENSNRISL"
BASE COUNT         1344 a         2444 c         2379 g         1264 t
ORIGIN      
        1 cttctcgcgg gttggttcgg tcacgccggc accggatcga gcacccgctc gcggatgaag
       61 gtctggagcg catcgcgatc gcgactcgcc tgcacgaacg cggtcaggta tgccaaatcc
      121 ggctcgtatt caccggcgca cccgaatggc catgccccgc gcggcgcgtc gacgaccgcg
      181 tccacgtaca gcgacgagat cgtggccagg gacggatcgg tctcgaacgc ggatccatcg
      241 acgattcgct cggcagtcag gatgacgaca tccgcagcct gtgccatcag cacatcctca
      301 aacagcgtgc cgacgatccg ggcgtttccg cactcgtctg cttcctgaac atggatgatc
      361 gccacgtccg gccggatcgc acggatcgcg accacctcct cgcccgtgta gggatcggca
      421 acggtccgga aatcgcgtgt ccgcaggaga tcggagccga agagcccggc caccggcata
      481 aacggcacgc cctggatcgc ggcacgcaga ccgctgatca ccgtgtagca ggcgtgctct
      541 gaggcgacca ccgtgccaga ttccaccgcg gcccgatagg acggagcgag cccgagcacg
      601 gtctcgtacc cgacgtagcc ggccgagacc gatgacgcgc agccggtcgc gcagagcagg
      661 tcgatatcgt acgagccggc ggtcttgatc acatccagac cgcgcttgcc ctgacgcgcg
      721 atttcgtgca cggccgcgcc gggtccacga tggatcgtgt tcccgccgag cgccaaccgg
      781 ccgccatcgg gaatccgccc gacggcctcg gacagggtcg taattttgga ttgcatggcg
      841 atcctcccgc gcgatcgatg cgcgtcagca actatcatac cgggcatgtc gtgaccgagc
      901 cgcttcggga ttccctgcca tgtatccacc tgaggtctac aagtcgtacc tgacgccgtt
      961 ccgattcctg caacggtcgg cagccgtttt ccgggagaaa gtcgcgatca tccaccgcga
     1021 ccaatgcatc acctatcccg agctcaatgc ccgggtcaat cggctcgcgt cggcgctgcg
     1081 caatgccggg atcgcgcgtg aggatcgggt ggcagtcctt ctgccgaata tcccgcagat
     1141 gctcgaggcc catttcggcg tgcccctctc gggcggaatc atggtcgcca tcaacacccg
     1201 cctcaagacc gatgagatcg cctacatcct ggaccattcg ggcgcaaagg ccctgatcgt
     1261 cgacagcgag ctggccccgc tggtcgagcc cgccttctcg gagttgctgg cgctggagac
     1321 ggtcatcacg gtgcacgacg tcgacgccgg catccacctc gaaggcccgg attacgaggc
     1381 atttctcgcg tccggatcgc cagaggagct cgattggccg atcgacgacg aggaccaggt
     1441 catctcgatc aattacacca gcgggacaac cggcaaaccc aagggcgtca tgtacaccca
     1501 tcgcggcgca tacctcaact cgctcggcga gttgatcgag acgcggatgt ccagcaccag
     1561 cacctatctc tggacgctgc cgatgttcca ttgcaacggt tggtgctacc cgtgggccgt
     1621 aaccggaatc gggggaaccc atgtctgcct gcgcaagttc gagccgggtg aggcatggga
     1681 cctcatccgc aaacacaacg tcacgcacct ttgcggcgcg ccgacggtcc tgatctcaat
     1741 ggccaatcac cccgccgctc cgacggagcc gctgccgcgg cagttgatcg tctcggttgc
     1801 cgccgcaccg ccgtcgccgt cgatcattgc ccagatggag tcgctcggga tcgagatcgt
     1861 ccacgtctac ggcctgaccg aggtctacgg gccatacacg gtctgcgaat ggcaggagca
     1921 gtgggacgag cggccggtcg aggagcggag ccggatcaag gcacgccagg gagtcggcta
     1981 tctggttgcc gatgacgccc gtgtcgtgga cgacaacatg gtcgatgttc cggccgatgc
     2041 ccagacgctc ggcgaagtga tcatgcgcgg caacaacacc atgaagggct atttccggga
     2101 cgacgaggcg acggaccggg cctttcgcgg cggctggttc cactccggcg acctcggcgt
     2161 gatgcacccg gacggctaca tcgaattgcg ggatcgcgcc aaggacatca tcatcagcgg
     2221 cggcgagaac atctcgacga tcgaggttga gcaagcgatc gccgcgcacc cggcagtgct
     2281 cgaggtggcg gtgatcggtg tgccggatga ttattggggc gaaactccga aggccttcgt
     2341 cgtcctgcgc gatggcgcat cggcgacgga gcaggaaatt atcgaccact gcaagtcgcg
     2401 gttggccggt ttcaaatgcc cgagggctgt ccatttcggc gagttgccga agacgtcgac
     2461 cggcaagatc caaaagttcg tccttcgcga gcgggagtgg gccggtcgct cgagcagaat
     2521 caactagcgc acaccggaga gcaccgcatg cggcacatca acatcgtctc ctatttgatc
     2581 ctcttgaccg gcctgattat gaccgtcgct ggagcggctc tcgatctcgg tgcggtcgtg
     2641 accctgatcg ggatgatgct ggtcgtcgcc gggattgtta agatcatcac cgttcggata
     2701 tggaacggct tcttcgatag cgacacggtc gccggggagt gacgcgggca tgagcgagca
     2761 gcgcgaggag aagcagccac ccgggtaccg cggcacgacc ggccggttag gcagcacgaa
     2821 tcgcacaccg gcagagcagg cccgcctcca gaaatcgcgc cagaaccgcc agatcttctc
     2881 cggcgccgag atccgccctc ccagccgggc cctgagtagc tacccggcgc agtccaccac
     2941 gctgacccag ccgcaattgc tctatgccgc ctcgctcggc atcctgctgg tgctcgggct
     3001 cctgctctgg ctgatcttcg ggatgggtgt ggccagcatc atcttcttcc tgctcgcgct
     3061 cggcctgctc ggcggctggc tggccttcta gatggccgcg atctcctggt ccgacatccc
     3121 ggcaaccgag gtctatccgg gcatcagtcg ccaaatcctg aacggcgatc gccaaacgat
     3181 ggtgcggtac gtctaccagc cggggtcgat cttccccgaa catcaccacc ccgaagagca
     3241 ggtcaccgtc gtgatctcgg gccggatcga attcacggtc gccggggagg ccgaggtgct
     3301 tgcgagtgga caggcagcgg tgatcccgcc ggatgtcccg cacggcgccc gcgtgatcgg
     3361 cgacgagccg gtcgagacgt tcacttcgat gagcccgcgc cgggagcagc atcccgcgcc
     3421 caatccagaa acgaggaacg aacggtgacg aagaaagagt ccggccgcgg ccatctgcgg
     3481 gtcgtcggga aatccgcccg gcagacccag tcgggaccgc agacctaccg tcccggccag
     3541 ttcggcacca tgttcgacct gacaggaaag ggtgcgctgg tcatcggcgg cggtggtgga
     3601 atcggtggcg cgattgccca tgccctggcc gatttcggcg cgcgggtcgc aatcgctgac
     3661 ctcagcctcg atgccgcgca ggcgatcgcg acctcctgcc agcgggatgg cgccgggacc
     3721 acaacctgtc gctcgatcga cgtcaccaac ccggatcacg taaaccaagt cgtggctgag
     3781 atcgaggcag agctgggcaa gatcgacatc ctggtcaact cagccggaat caacatccgc
     3841 aaggacgtga ccgattacac gcccgaggag tggttcaaga tcatcaatac gaacctctcc
     3901 ggcgtcttct tcgtgaccca ggcggcggca cgggggatgc tcgcgcgcgg ctacggccgg
     3961 gtgatcagca ttggctcggt ctcgagcctg atggggcatc cgcaacatgc gccctacgcg
     4021 gccaccaagg gcggggtcgc gatcatgacc aaggcgatgg cgaccgagtg ggcgatgaag
     4081 ggcgtcacgg tcaacgcgat cggccccgcc tacaccgtga ccggcctgat cacggagctg
     4141 atgaacaacc cggtcaaata ccgcgagatc atcgagacga tcccgatggg tcgcctcggc
     4201 aatccagagg acatcgtcgg ggcggcggtc tacctggcct ccgacgccgc cagcttcgtc
     4261 accggacaga cgatctacgt cgacggcggc cgcaccgcgg actagcaagc aatagcgtgg
     4321 cgcggttgac accggcggtt ggcattgggc actgcacgtt tggcgtcgcc ccggactgaa
     4381 ggccggggct gacccagaat aaggcggctg aagccgactg cttttcagcc ccgtcagtgg
     4441 gcttagcgtg ggtcagcccc ggcctttagc tccgggcggc accggaatcc cggtcgagaa
     4501 cgcgcgccaa gtctccggtc gcaccgggtt gccgcgttgc tccatcgcgg gatgctcttt
     4561 ctctggtagg gagagcccga gagggtagag aggcatccgc gccacgtcga atggccaatt
     4621 cacggattac ccaagagcgg cttgccatcc tggtagagga ggtcgagcat gcggttttgt
     4681 cgctccatgg ctgcccggta gacctcggcc cggccggggt cgggttcgat cgtctgcccg
     4741 tacccggctg ggtcgtcggc atcgaagagc gacttgatca atccggcgta ctcgagcgcc
     4801 atgagggcgg cgcctcgcgc ggtcgactcc tcttcgggag gcagtgcgac gaccgagtgc
     4861 tgcgtcacgt cggcgatcat ctgcatccac accggcgacc gaagaattgc gccgccatta
     4921 gccaggatct ggtgatccgg ctcagcctgc gtggcgagat cggcatagat catgcccaac
     4981 cggtacgaga ccgactccat cccggcccgg atcaggtcgg ccgcggtcgt ggccaagttc
     5041 agcccgctga ccgcgccggt cgcccagtcg ctccagatcg gcgcccgctc gccggcgagg
     5101 aacggcagga tcgtgagacc gtgcgagtcc ggttccagct gctcagcgca gcggaacgcc
     5161 tcactgccga atgcggagcc ggtcaggtcg ccgatccaat cgactacctt gccgccgtta
     5221 ctgatcgccg ccccgatcac cgctgcccgg tcgtcgagcc ggtaggtcca cagggtttcc
     5281 ggggtatttg gcgagcttcc ggccggtagt cgccggacca tccgcatcgc gccggaggtg
     5341 ccgaccgtca gcgcgatccg attcggaccg agcccacccg agccgacatt ggcgcaggcg
     5401 ccgtctccga tcccgcgaaa ccagggagcc tcagcgagcg ctggccaccg cctggagaac
     5461 tccggggcca tgccggtcag cggctcgcgt tcgtggcgaa tcggcgccag ccggtcgggg
     5521 tcgacgcccg aagccgcgat cgccaggtca tcccattggt gcgtcgtcgc gttcatcatc
     5581 gcggtcgacg acgccatcga caccgatatg ccgtcggcgc cgaacagctt tcgcaggagg
     5641 tatccggtga aggagcacca ggtcctcgtc gtgcggacca gatccggttg ggtgcgggcg
     5701 agccagcgga gcttgccggg ccagtagctc gagtggacga agcagccggt ccgctgccag
     5761 agttcgtggg cgtcgaactc ctgtttgatc gcgtcgacgt cctcccgaga tcgagtgtca
     5821 gcccagaggt agaccggcgt agtcggctca ccgtcggcat cgactcccat cagcgaatgc
     5881 cagaaggcgg cgacgccgac cgcggcgatc ggcaccttgt gccgctccag caccgcgtcg
     5941 atcgatttcc cgaccaggtc ggccagcatg cgggcatcga cttccgcgcc gccgtcgggc
     6001 gtcgtggtca gctcgtactt gagctggcat tcgctgtcgc gcagggcatg gccgcggcgg
     6061 tcgaagatga tcgcgcgggt agaggacgtg ccgatgtcca gggccaggac cagcggctgt
     6121 tcggcattct ggacgtcgac gactggcact ggatcctcct cgattgcacc tgatccgagt
     6181 atacggagga gccgcgtttt gcccgggcgg tgtgaaacgt atcgggcact actcgtcgtt
     6241 tatatgggtg atgattggtt tttggggacg gatcacacca tgaacgagca cagctttgat
     6301 caactcgcgc ggaaccttgc tcaccagacc gatcgccgaa cgatcctggg cagcgtgatc
     6361 gccgccggcg ccgcattgat cggcattcgg cacgccgcct tcgcgacacc ccagagtcag
     6421 ggcggtctcg tcttcgacta ctaccaggcg atcgaccgcc gggcgttcaa gaccgcgtac
     6481 ggctacctcg gcgcgaagtt ccgcgcccgc cagacgctcc agaacttcac gaccggcttc
     6541 tccgacacgg tgtacgacga ccttgtgatc accaacgtcc gcccagacgc aatgcgcaac
     6601 caggtcgtct acgacgtgac cgtcaccgcg tggcatttag atggctcggt ccaccgcttt
     6661 accggcacct acaccgaggg ccaggaagcc ggagcgacga agctgatcga cgccgtgatc
     6721 agggaggtac cggtccacga tatgcctccg ctctgcgccg cgagcgacct cgaggcgacc
     6781 atgacgggtg atgccggtgc gggtcagcgc tatggcactg ttaccgcgac gaacgtcgcc
     6841 gccggctcct gcgtgctcgg cggtatcccg agagtcgtgg tgaccgacgc ggccggtaac
     6901 cccgtgatct cggcccgacc ggaaccgaac gtcccgatca cgaccgtcac gctcgctccg
     6961 ggcaagagcg ccacgctcgg catccattgg tcgaactggt gcggaacacc ggtcagcgga
     7021 gagccacagg tctcggtcat gctccgtggc ggtgagggcc gtatcacgaa cctgaccggt
     7081 ctcgctgtgc cgccgtgcct gagcgaaccc ggtggtgcat cgaccctgaa cgtcaagccc
     7141 tggcaaatcg gttaggctgg caatcggttg acggccccgg cggcctctcc tagcattact
     7201 ttccgaattg aagctttatg agtacgaagg ggattcctgt tcgatgctga gtgatgccgt
     7261 ccgcgatttt ctgaacgagc gccgtttcgc cgtcctggcc acctccaatc ccaatgggac
     7321 aatccagcaa tcggtcatgt ggtacctgct ggaggacaac acgatcgtga tgaacaccgc
     7381 caaggggcgg atcaagttcc ggaatatgga gaattcgaac cggatctcgc t
//
LOCUS       DATAOZ010000170         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2177393, whole genome shotgun sequence.
ACCESSION   DATAOZ010000170 DATAOZ010000000
VERSION     DATAOZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177393"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VHR64_04675"
     CDS             complement(<1..556)
                     /locus_tag="VHR64_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HEX3302153.1"
                     /translation="MSELLTTASTLASVAAEPEGSRVAPTASYGDLQLVIEDVPIGDL
                     KSYARNARTHSKAQIRQIADSLLGFGFVSPIVVDRDGTIIAGHGRVAAAKLIGRKTVP
                     TVRADHLTDDQVKALRIADNRLAELAGWDSNLLALEFNDLLEIDLKAELSFDLAITGF
                     APAEIDRMVEAARENEEAAKDDAVP"
     gene            complement(829..1989)
                     /locus_tag="VHR64_04680"
     CDS             complement(829..1989)
                     /locus_tag="VHR64_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEX3302154.1"
                     /translation="MKQRRRIYYSAAQRAEIWDRWKAGESMSSIGRRFDRESSSVFSV
                     LSATGGMRPPDRTRASRALSLSEREEISRGLSIRRSLRGIARLLGRSPSTISREVLRN
                     GGPERYRATGSDQAAWDRALRPKRCKLACRPALARTVSGKLRRNWSPEQVAGWLKRAW
                     PGEPHNQVSHETIYRSLFIQARGVLKKELLTHLRARRTIRRSRHATLKRNGLGQIKDA
                     VSISQRPASVEDRAVPGHWEGDLIGGSRNSYVATLVERHSRYVMLVKIANNDTESVVS
                     ALIKQSRKLPGELYQSLTWDRGKELADHKRLALASNVEVYFCDPRSPWQRGSNENTNR
                     LLRQYLPQGTDLSLQSQAQLSAIARQLNERPRKTLLYRTPAETFAECVAAIS"
BASE COUNT          378 a          676 c          730 g          413 t
ORIGIN      
        1 ccgggacggc atcgtccttc gccgcttcct cattctccct cgccgcctcg accatgcgat
       61 cgatctccgc cggggcaaag ccggtgatgg caagatcgaa ggagagctcc gccttaaggt
      121 cgatctcgag gaggtcgttg aactcgagag ccaggagatt ggagtcccag ccggcgagct
      181 cggccagccg gttgtcggcg atgcgcagcg ccttgacctg atcatcagtc aagtgatcgg
      241 cgcggacggt cggcaccgtc ttgcgaccaa tcaacttggc ggccgcgacg cggccatgcc
      301 cggcgatgat ggtgccgtca cgatcgacga cgatggggct gacgaagccg aaaccgagga
      361 gcgagtcggc gatctggcgg atctgcgcct tcgagtgcgt gcgcgcgttg cgcgcgtacg
      421 acttcaggtc gccgatcggc acatcctcga tcaccagctg gagatcgcca tacgatgcgg
      481 tgggggcgac gcgcgaacct tccggttccg cagcgacgga cgcgagggtg gaagcagtgg
      541 tgagcagttc gctcatagca gtcctcatac atttgcgcga ccccgctgcc gtgaccggca
      601 acggtgcccg agaggccgaa gccccgggaa ggtcgtctgc aaatgcccag cgaggggact
      661 gctgctcgac gaatgacgag cgctatagat caggagggag gaatgctgtc aagggcctgg
      721 gaaagaatgc cttcagatgg tgacatttcc gacgcgggtc cacgagcggg cgcggccagg
      781 gctggccgat agcgggaggt ccgcttcagc ggttgatcga tggtgggttc aactgatcgc
      841 tgcaacacac tcggcgaacg tctccgctgg ggttcgatag agcaaggttt ttcttggccg
      901 ttcgttgagc tgccgggcga tggcgctgag ctgggcttgg ctttgcaggg ataggtcggt
      961 cccttgaggc aggtactgtc gcagcagccg attggtgttt tcgttggagc cgcgttgcca
     1021 gggtgatctg ggatcgcaga agtagacctc gacgttcgag gcgagagcca ggcgcttgtg
     1081 atcggcgagt tccttgcccc gatcccaggt cagggactga tagagttcgc cgggcagttt
     1141 ccgggactgt ttgatcaggg cggagacgac gctctcggtg tcattgttgg cgatcttgac
     1201 cagcatcacg tagcgcgagt gacgctcgac cagcgtggcg acgtagctgt tccgggaccc
     1261 gccgatcaag tcgccttccc aatggccggg cacggcgcga tcctcgacgg atgcgggccg
     1321 ttggctgatg gagaccgcat ccttgatctg gccgaggccg ttccgtttca gggtggcgtg
     1381 ccttgagcgc cggatcgttc gtcgggccct gaggtgggtc agaagctcct tcttcagcac
     1441 gcctcgcgct tggatgaaca ggctgcggta gatggtttcg tgcgacacct gattgtgcgg
     1501 ctccccgggc caagcgcgct tgagccaacc ggcgacctgc tcgggcgacc agttccgccg
     1561 cagcttgcct gatactgtcc gggccagggc tggtcggcaa gccagcttgc agcgttttgg
     1621 acgcaaggcc cggtcccagg ccgcctgatc ggagcctgtc gcgcgatacc gttccggccc
     1681 gccattgcgc agaacctcgc ggctgatcgt tgatggcgat cgccccaaca gccgagcgat
     1741 ccctcgcagc gagcggcgga tgctgagccc tcgggagatc tcctcccgct cgctcagact
     1801 gagagcgcgc gacgcccgcg tccggtctgg aggccgcatg ccgccggtcg cagaaagaac
     1861 cgaaaacacc gaggaggact ctcgatcgaa acgccgtccg atcgaactca tcgactcgcc
     1921 ggccttccaa cgatcccaaa tctcagcccg ctgggccgcc gaataataga tccgccgccg
     1981 ctgcttcatc acccacgctc catctccgaa ggaaaagtag cgtgttgcgt tcaccccttg
     2041 agcccaccgc cgaatgcgga cgttccattc tcggctactt gttgtcgggc gctttgcgcc
     2101 cattcctgat gtgcaaatgg ttgcggcggc agcggaccta gatgtccgca tagacatgag
     2161 tttcggcttg cccgcccgga tgcgtcacgg cacccga
//
LOCUS       DATAOZ010000171         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5860747, whole genome shotgun sequence.
ACCESSION   DATAOZ010000171 DATAOZ010000000
VERSION     DATAOZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5860747"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VHR64_04685"
     CDS             complement(<1..635)
                     /locus_tag="VHR64_04685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3302155.1"
                     /translation="MRLALDIGPEQWRKPGGVQRSIELAQRADELGFDTIWASEDPDG
                     WDAFSALSVLARSTQRIHLGTGVTNPYYRHPNLISASVATLDHASDGRAFLGLGRGEP
                     DWYRTAFGMEIGSPLGRVRETVDLLRQWWSPEQRATGGGEITVRAWRREFSPVGNPLI
                     YIAATGPQMQALAGEIGDGIRFNLLGSLPFLKRGIETFLGAARSVNHDPADL"
     gene            complement(639..911)
                     /locus_tag="VHR64_04690"
     CDS             complement(639..911)
                     /locus_tag="VHR64_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302156.1"
                     /translation="MSEAPSIPNPLCEIGRTHPRDKHRMKPLGDYPGVWYCPKHELYA
                     TVLPEDVADDIERGSTYQMHDGTEGVAGRTGDERPGGIVFYYRPQR"
     gene            1050..1478
                     /locus_tag="VHR64_04695"
     CDS             1050..1478
                     /locus_tag="VHR64_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX3302157.1"
                     /translation="MSQPIPPSARALIDSAPLAHFVTINPDGSPQVSCVWIGIDDDRV
                     YFASLTAHQKLRNIQRDNRVVLSIESPTEAAWGVQEYLVLYGHAEVSQGGGAEALQRL
                     AYRYMGPDAVFPDMESPPAGFVVWVTVDRIRGVGPWMEGN"
BASE COUNT          265 a          536 c          492 g          265 t
ORIGIN      
        1 aggtcggcgg gatcgtggtt gaccgaccgt gcggcgccaa gaaacgtctc gatgccccgc
       61 ttcaggaaag gcagcgagcc gagcaggttg aagcgaatcc cgtcgccgat ctcgccggcc
      121 agtgcctgca tttgcggtcc agtcgcggcg atgtagatca gtggattacc gaccggcgag
      181 aactcccgcc tccacgcccg gaccgtgatc tcgcccccgc cggtcgcgcg ctgctccggg
      241 ctccaccact ggcgcaggag gtcgaccgtc tcccgaacac gaccgagcgg cgagccgatc
      301 tccatcccga acgcggtccg gtaccaatcc ggctcccccc ggccaagacc aagaaacgcc
      361 cggccatccg acgcgtgatc gagcgtcgcg accgaggccg agatcagatt cgggtggcgg
      421 taatacggat tggtgacccc ggtgccgagg tgaattcgct gggtcgaacg ggcaagcacc
      481 gacagcgcgc tgaatgcgtc ccagccgtcg ggatcctcgc tcgcccagat cgtgtcgaag
      541 ccgagctcgt cggcccgctg agcgagctcg atcgaacgct gcacgccgcc cggcttgcgc
      601 cactgctccg ggccgatgtc aagcgcgagc cgcatcgtct agcgctgggg ccggtagtag
      661 aagacgatcc cgcccggccg ttcgtcgccg gttcgtccgg ccacgccctc ggtgccgtca
      721 tgcatctggt atgtcgagcc gcgctcgata tcgtcagcca catcctcggg cagcaccgtc
      781 gcgtagagct cgtgcttcgg gcagtaccag acgccggggt agtcgccgag gggcttcatg
      841 cggtgcttgt cacgcggatg cgtccgcccg atttcacaga gcggattcgg gatcgatggt
      901 gcttcgctca tacggcgttg ggtgtcctcc cggaaccaaa atcaggtcat acttccgaaa
      961 agtatgccgc cattcgccgg gggacaccac gtgaccgcgt acatcaaaac cgtcgtaagt
     1021 gcacgatttt gcctggcgga gccggtccga tgagccaacc aattcccccc tcggcgcgcg
     1081 ccctgatcga ctccgcaccg ctcgcccact tcgtcacgat caaccccgat ggctcgcccc
     1141 aagtctcctg cgtctggatc ggcatcgacg atgaccgcgt ctacttcgca tcgctgacag
     1201 cccaccagaa gctccgcaac attcagcgcg acaaccgggt cgtgctcagc atcgagtcgc
     1261 cgaccgaggc agcctggggc gtgcaggaat acctcgtgct ctacggtcac gccgaggtca
     1321 gccagggcgg cggcgccgaa gcgttgcaac gcctcgccta ccggtacatg ggaccggatg
     1381 ccgtcttccc ggatatggag agtccgccgg ccggcttcgt cgtctgggtc acagtcgatc
     1441 ggatccgcgg cgtcggcccg tggatggaag ggaactaggg ttgcacgatg agtagttccc
     1501 cgcttgacgt tgccacggtg cgcctcgatt cgtacgacgt cctggccccg gatggctg
//
LOCUS       DATAOZ010000172         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8754443, whole genome shotgun sequence.
ACCESSION   DATAOZ010000172 DATAOZ010000000
VERSION     DATAOZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8754443"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..>1618
                     /gene="atpA"
                     /locus_tag="VHR64_04700"
     CDS             74..>1618
                     /gene="atpA"
                     /locus_tag="VHR64_04700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX3302158.1"
                     /translation="MATESILRPGELKEVLLREIASADLATEDVSEVGTVLEVRDGIA
                     RIYGLKSAIASEMLEFTAQETGDTVAGLVLNLEEDNVGAAIMGDYLKLKEGDEVRRTG
                     RLLDVPVGPGMLGRVVDALGNPVDGRGPIQASGRRQVEMVAPGIIVRQPVKEPLQTGI
                     KAIDAMIPIGRGQRELIIGDRGTGKTAIAVDTIINQKGTGVVCVYVAIGQKRSTVASV
                     VDKLKDAGALEFSIVVIASASDPAPLQYIAPYAGCAIAEYFMYEEGKATLCVYDDLSK
                     QAAAYRQLSLILRRPPGREAYPGDVFYLHSRLLERAAKISDNPDVTRLDPRIKKPGGS
                     LTALPIIETQAGDVSAYIPTNVISITDGQIFLTTDLFYSNVRPAVDAGISVSRVGGNA
                     QIKAMKQVAGPLRLSLAQYRELEAFAQFGSDLDAATQRQLARGARLVEVLKQPQYRPV
                     PVENQVVIIYAVTNGYLDDVEVPHIRQWERDFLDYLEAQHPAVLKDIRTKKALDDDLT
                     NRLKNAI"
BASE COUNT          300 a          519 c          520 g          279 t
ORIGIN      
        1 ttccccgtct gtaccattgt gactattttc acaagctaca tttgaagcct acctcagccc
       61 ttgttcacga ctcatggcta ctgaatcgat tctgcgtccc ggcgagctca aggaagtcct
      121 gcttcgggag atcgcgtccg ctgatctcgc gaccgaagat gtcagcgaag tcggcaccgt
      181 gctcgaagtg cgcgacggca tcgcccgcat ctacgggctc aaatccgcga tcgcgagcga
      241 aatgctcgag ttcacggccc aggagaccgg cgatacggtt gccggcctgg tgctgaatct
      301 cgaggaagac aacgtgggcg ccgcgatcat gggtgactac ctgaagctca aggaaggcga
      361 cgaggtgcgg cgcaccggcc gtctcctcga cgtccccgtg gggcccggga tgctgggccg
      421 agtggtcgac gcgctgggaa atccggtgga cgggcggggg ccgatccaag cgagcggccg
      481 ccgccaggtc gagatggtgg cgccgggcat catcgtgcgg cagccggtga aggagccgct
      541 gcagaccggc atcaaggcga tcgacgccat gatcccgatc ggccgcggcc agcgcgagct
      601 gatcatcggc gatcgcggca ccggcaagac cgcgattgcg gtcgacacca tcatcaatca
      661 gaagggcacc ggcgtggtct gcgtctacgt cgcgatcggc cagaagcggt cgacggtggc
      721 gagcgtcgtg gacaagctga aggatgccgg agcgcttgag ttcagcattg tggtcattgc
      781 ctccgcctcg gacccggccc cgctgcagta catcgcgccg tacgccggct gcgccatcgc
      841 cgagtatttc atgtacgaag aagggaaggc gacgctctgc gtgtacgacg acctgtcgaa
      901 gcaggccgcg gcgtaccggc agctctcgct gatccttcgg cgtcctcccg gccgcgaggc
      961 gtacccgggc gacgtgttct accttcatag tcgccttctg gagcgcgccg ctaagatctc
     1021 cgacaatccc gatgtgaccc ggctcgatcc ccgcatcaag aagccgggtg gctcgctcac
     1081 ggcgctcccc atcatcgaga cccaggcagg cgacgtctcc gcctacatcc cgaccaacgt
     1141 catctcgatt accgatgggc agatcttcct caccaccgat ctgttctact ccaacgtgcg
     1201 tcctgcggtc gatgccggca tcagcgtgag ccgagtggga ggcaatgcgc agatcaaggc
     1261 gatgaagcag gtggcggggc cgctgcgact gagcttggcg cagtaccgcg agctggaggc
     1321 gttcgcgcag ttcggctccg acctcgatgc cgctacccag cggcagctcg cgcgtggggc
     1381 tcggctggtc gaggtgctca agcagccgca gtatcgcccg gtaccggtgg aaaaccaggt
     1441 cgtcatcatc tatgcggtga ccaacggcta tctcgacgac gtcgaggtgc ctcacatccg
     1501 ccaatgggag cgcgatttcc tggattacct cgaggcgcag catccggcgg tcctcaagga
     1561 tatccgtacc aagaaggcgc tggacgacga tctgaccaac cggctcaaga acgcgatc
//
LOCUS       DATAOZ010000173         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3491815, whole genome shotgun sequence.
ACCESSION   DATAOZ010000173 DATAOZ010000000
VERSION     DATAOZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3491815"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VHR64_04705"
     CDS             <1..451
                     /locus_tag="VHR64_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3302159.1"
                     /translation="LAGEVGSIEPGKLADVVIWHPAFFGVKPELVIKGGIIGWAAMGD
                     PNASIPTPQPVRYRPMFGALTPEATSVSFLSAAALEAGVPEQLGLRKRAVAVRETRTI
                     GKSSMIHNDATPHITVDPETYEVRADGELLVCEPAETLPMAQRYFLF"
     gene            469..1161
                     /locus_tag="VHR64_04710"
     CDS             469..1161
                     /locus_tag="VHR64_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEX3302160.1"
                     /translation="MSESARLLRLLQLTDSGFPTGAFAFSHGLEGLVSSGFVQSERDV
                     EQFIIAQLREGFGGIECPAMAHAWRASRQANVDALLEIDTLVDALKPVPVFKSGSVRT
                     GRRLLESASGLLAGPMLADLRMAVRSGKTSGHHAVAFGVVMESAGQDLETASLALGAG
                     FVNGLGAAAVRLGVIGQVAAQRIVGRSSNAIDAVAARGRGMALEEMGSYLPMIDVAGL
                     RHPLLAGRVFAS"
     gene            1158..1781
                     /gene="ureG"
                     /locus_tag="VHR64_04715"
     CDS             1158..1781
                     /gene="ureG"
                     /locus_tag="VHR64_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEX3302161.1"
                     /translation="MSAGRIPVRIGVAGPVGTGKTALVWRLCESLRDRVSLAVITNDI
                     YTKEDAEFLTRHAALSVERSVGVETGGCPHSAIRDDPSLNEEAIAELIIRHPGLDLVL
                     LESGGDNLSATFSPELVDASIFVIDVAGGDKIPRKGGPGICRSDLLVINKTDLAPFVG
                     ASLEVMQRDATLQRGNRPFVFTNLRDGEGVPEILTWLRPLLPEPLDA"
BASE COUNT          353 a          594 c          630 g          398 t
ORIGIN      
        1 tctcgctggc gaagtcggct cgatcgagcc tggcaaactg gccgacgtcg tcatctggca
       61 ccccgcgttc ttcggtgtca aacctgagct ggtcatcaag gggggcatca tcggttgggc
      121 ggccatgggt gatcccaatg cctcgatccc cacgcctcaa ccggttcgct atcggcccat
      181 gttcggtgcg ttgacgccag aagcaaccag tgtcagtttt ctttcggcgg cggcgctgga
      241 ggccggagtg ccagagcaat tggggctccg caagcgggcc gtagcagtca gagagacccg
      301 caccatcggc aagtcgtcga tgatccacaa cgacgcaact ccgcatatta cggtcgaccc
      361 tgagacctat gaggtgcgcg ccgacggcga gctgttagtc tgcgagccag ctgagacgct
      421 gccgatggcg caacggtatt tcttattttg acgcaaggca ctcaagacgt gagtgaatcc
      481 gccaggctgc ttcgtctgct tcagctgacc gattcaggat ttccgaccgg agccttcgcc
      541 ttctcgcatg gtctcgaagg tctcgtatcc tcaggatttg tccaaagcga acgcgacgtc
      601 gagcagttca tcatcgctca gcttcgtgaa gggtttggag ggatcgagtg cccagccatg
      661 gctcatgcct ggcgagcaag tcgccaggcc aacgtcgacg ctctgctcga gatcgatacg
      721 ctcgtcgatg ccttgaagcc ggtgccggtc ttcaagagcg gcagcgtgag gaccggccgt
      781 cgtttgctcg agagcgcctc tgggttgctt gccgggccga tgctggcaga cctccgaatg
      841 gcagtccgta gcggcaaaac ctctggccac cacgccgtag cttttggcgt cgttatggag
      901 tcggcagggc aggaccttga aaccgcgtcg ctggcgcttg gcgccggctt cgtgaacgga
      961 ttaggcgccg ccgcggtccg gctcggggtg atcgggcagg tcgcggctca acgcatcgtc
     1021 gggcgatcga gcaacgcaat cgatgccgtc gccgcacggg gccgggggat ggcgctcgag
     1081 gagatgggat cgtatttgcc aatgattgat gtggcaggtc tgcggcatcc tctgctcgcg
     1141 ggcagggtgt ttgcctcgtg agcgcaggac gaatcccggt acgtatcggc gttgccggcc
     1201 cggtaggaac ggggaagacc gccctcgtct ggcgactctg tgagtcactg cgcgaccgtg
     1261 tcagtctggc tgtgatcacg aatgacatct acaccaagga ggatgccgaa tttctgactc
     1321 gacacgccgc gctctcggtc gaacggagtg tgggagttga aactggcggg tgtccccact
     1381 ccgccattcg cgacgaccct tccctcaatg aagaagcgat tgccgagctc atcattcggc
     1441 accccggatt ggatctcgtg ctgctggaga gcggcggtga caacctctca gcaacgttta
     1501 gtcctgagct cgtcgatgcc agcatcttcg ttattgacgt tgctggggga gacaagattc
     1561 cacgcaaagg cggtccgggc atctgccgtt ccgatctgct ggtcatcaac aagaccgatc
     1621 tggcgccttt cgtcggagcc tcactcgagg tcatgcagcg tgatgccacg ttgcagcgag
     1681 gcaaccgccc attcgtgttc acgaatctcc gcgacgggga gggtgtaccc gagattttga
     1741 cctggctgag gcctctgctg ccggagcccc tcgacgcgtg acgtccgcga ccgccggaca
     1801 gttgcgcatc gacgcgcagg caaaggccgg ccggacgtat ctcgctgacg tactagcgac
     1861 acccccgttt cacccaggac ccttgcaccg acggtccggc atggccgaga tgatcgtgca
     1921 gtcggttggg ccgggagtgt ttccaggcga aagcctgttg acaacgatca ccgtt
//
LOCUS       DATAOZ010000174         5709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7965559, whole genome shotgun sequence.
ACCESSION   DATAOZ010000174 DATAOZ010000000
VERSION     DATAOZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7965559"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1500)
                     /locus_tag="VHR64_04720"
     CDS             complement(19..1500)
                     /locus_tag="VHR64_04720"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3302162.1"
                     /translation="MATALKDFRIQLTDPSLFREQCYVDGAWVDADNRATSEVTDPAS
                     GEVIGTVPRMGAAETRQAIEAADRAFKGWSRTPAKERSKILRKWFDLMMANQEDLAIL
                     MTREQGKPLAESRGEIAYGAAFVEWFAEEGKRVYGDIIPTVAESRRLMVFKQPVGVCA
                     AITPWNFPNAMITRKCGPALAAGCTVVVKPASFTPYSALALAELGERAGIPKGVFNVV
                     TGSAKEVGGELTSNDTVRKLSFTGSTEVGKILLKQCADTVKKVSMELGGHAPLIIFDD
                     ADLEAAVKGAIACKFRNSGQTCVCTNRIYVQEKIYDAFAEKFAAAVARLKVGAGLEPG
                     VEQGPLIEPAAIAKVERHVSDAKSHGAEVLTGGQRHALGGLFYEPTVLSNCTAEMLIS
                     HEETFGPVAPLFKFKDEDEVIGLANKTEYGLASYFYARDVGRVFKVAEALEAGIVSVN
                     EGIFSTEVAPFGGYKHSGIGREGSKYGIDEYLEIKYVNLGGIS"
     gene            1702..2181
                     /locus_tag="VHR64_04725"
     CDS             1702..2181
                     /locus_tag="VHR64_04725"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3302163.1"
                     /translation="MTNQRPAPALDSKDLPGRQSSGYPEPFRAAVAARIKRALGDPFG
                     LTQFGVNLVELPPGCWSSQRHWHSHEDELVYVVSGELVLVTDAGETRMRAGMVAGFPA
                     GRQDGHHLINRSDAPATYLEIGSRIPADGAVYSDIDMEVRPRPEGGHVFVHKSGEPY"
     gene            2310..2765
                     /locus_tag="VHR64_04730"
     CDS             2310..2765
                     /locus_tag="VHR64_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX3302164.1"
                     /translation="MADQPAEDGASLYERLGGVYSIATVVDDFIDRIMVDPRLNANPR
                     VDEAHHRVPPAGFKYLVTEMLCWAAGGPQRYTGRDMEEMHRHLLITADEWQAFMDDLQ
                     QTLDKFRVPAAEQAEVRAIVDSTRSGIVVPKAAPEIVRGSDTVGQGLRA"
     gene            2762..3550
                     /locus_tag="VHR64_04735"
     CDS             2762..3550
                     /locus_tag="VHR64_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX3302165.1"
                     /translation="MTTVGQVESLWRYPVKSMRGEELAEAFIGFAGVYGDRLFAFKSS
                     SRPAGFPYLTGREQESMLLYRPRFRDPDKAAKPPNLAEAEAIAPGITPLPGAPADLVV
                     EVETPSGEVLAVDDPALTRRLGEGSPDGRELTLLRSERAMTDCRPVSLFSIQTARQLT
                     QETGIALDKRRFRANVYLDLRDAQGFVEDGFVGRRLRLGRKAVIAVLERDPRCKMITL
                     DPETAKPIPDILAKVTRVHQGMAGVYAAVLVEGTVRQGDDVDLI"
     gene            3696..5399
                     /locus_tag="VHR64_04740"
     CDS             3696..5399
                     /locus_tag="VHR64_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3302166.1"
                     /translation="MYGMVNLAIQEFLCDRYGHDRWTAIRATIPKETDRFLTMEQYPD
                     QLSFDIMTAASDLIAKPISQLLEELGEYWIAFAQRSGYGDLMDVLGEDLFEALQNLDN
                     LHTRVGFAFPDLRPPSFWLTDIEDDTLILHYQSVRSGLAPMVIGWCADWHAASTKMSA
                     YGKSSRLAAITVYSKLRLYNLTKIVDKSELHPRTGLIIMYGLNFRQFARIHPFHLVID
                     ADMHIVQAGTALRKIFTDLIGKKFYQHFEIVRPRLTLRFETIREAGNAAFLLGCPSLQ
                     MNLKGQMLADESRRLLFFLGAPLIESATLLRRHHLTLTDFPMVDSSIDILVSQQQQLV
                     NKKLENLILERTEDLEKERKIAERLLHAILPAHIVGNLKNRPSAIAEQFEQVTVMFLD
                     LVGFTPLTANMPAIAVVNLLNGIFQRFDELVEATDVEKIKTIGDAYMCVGGLSGASAQ
                     QSAERVALAGLQMLRIIDETVRAERLQARIGMHTGPAVAGVIGATKFAYDLWGDTVNT
                     AARMESHGEPGKIHCTREVYQLLGENFEFEARGEHHIRGKGRMQTYFLLGEKNDRAGA
                     D"
     gene            complement(5521..>5709)
                     /locus_tag="VHR64_04745"
     CDS             complement(5521..>5709)
                     /locus_tag="VHR64_04745"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX3302167.1"
                     /translation="TENIAFPRAFQLDPSGKWLYVCNQKGASIVQLAIDQKTGKLTPT
                     GNITEVPTPVSLVFTTGR"
BASE COUNT         1072 a         1735 c         1822 g         1080 t
ORIGIN      
        1 cacgcaagtc gaggcaggtc aggagatgcc gccgaggttg acgtatttga tctcgaggta
       61 ctcatcgatg ccgtacttcg agccctcgcg gccgatgccc gagtgcttgt agccgccaaa
      121 cggcgccacc tcggtcgaga agatgccttc gttgaccgag acgatgccgg cctccagcgc
      181 ctcggcgacc ttgaagaccc ggccgacgtc gcgggcgtag aagtaagagg cgagcccgta
      241 ctcggtcttg ttggcaaggc cgatcacctc gtcctcgtcc ttgaacttga agagcggcgc
      301 caccggcccg aaggtctcct cgtggctgat cagcatctcg gccgtgcagt tggagagcac
      361 cgtcggctcg tagaacaggc cgcccagcgc atgccgctga ccgccggtga gaacctcggc
      421 accgtgcgat ttggcatccg acacgtgacg ctcgaccttg gcgatcgccg cgggctcgat
      481 cagcggcccc tgctcgacgc ctggctcgag gccggcgccg accttgagcc gggcgaccgc
      541 ggccgcgaat ttctcggcga aggcatcgta gatcttctcc tggacgtaga tccggttcgt
      601 gcagacgcag gtctggccgg agttccggaa cttgcaggcg atcgcaccct tgaccgcggc
      661 ctcgaggtcc gcgtcatcga agatgatgag cggcgcgtgg ccgccgagct ccatgctcac
      721 cttcttgacc gtgtcggcgc actgcttgag caggatcttg cccacctctg tggaaccggt
      781 gaacgagagc ttgcgcacgg tgtcgttgct ggtcagctcg ccgccgacct ccttggcgct
      841 gccggtgacg acgttgaaca cgcccttggg gatgccggcg cgctcgccga gttccgccag
      901 ggcgagagcg gagtaggggg taaagctcgc gggcttgacc acgacggtgc agcctgccgc
      961 aagcgccggt ccgcatttgc gggtgatcat cgcgttcggg aagttccagg gcgtgatcgc
     1021 ggcgcagacg ccgaccggtt gcttgaacac catcagccgg cgactctcgg cgaccgtcgg
     1081 gatgatgtcg ccgtagaccc gcttgccctc ctcggcgaac cactcgacga aggcagcgcc
     1141 ataggcgatc tcgccgcgcg actcggcgag tggcttgccc tgctcgcggg tcatcaggat
     1201 cgcgaggtcc tcctgattcg ccatcatcag gtcgaaccac ttgcgcagga tcttcgagcg
     1261 ctccttggcg ggggtcctgc tccagccctt gaaggcgcga tcggccgcct cgatcgcctg
     1321 cctggtctcg gccgcaccca tcctcggcac cgtgccgatc acctcgcccg acgccggatc
     1381 ggtgacctcg ctggtggcgc gattgtcggc atcgacccag gctccgtcga cgtagcactg
     1441 ctcgcggaac aggctgggat cggtcaactg tatccggaag tctttgagcg cggtcgccat
     1501 gcgggcctcc tcctgtcgtg acacggcgct gcggcctcgc cgggccgcgc gcttcatgga
     1561 tgatctggcg gaatctggcg cccggactgc aggcgccgat gaggctgcct aaagcgcgac
     1621 ggcgggcgcc ggccatgacc tcgacatggg aactccgggc ggctattcaa ccggtcgata
     1681 atccaaaggc gaggcaatcc gatgaccaac cagcgaccgg cgccggcgct cgactcgaaa
     1741 gatttgcccg ggcggcaaag ctcgggctat ccggagccct tccgcgcggc ggtcgcggcc
     1801 cggatcaaac gggcgctggg cgatcccttc ggcctgaccc agttcggggt caatctggtc
     1861 gagctgcccc ccggctgctg gtcgtcgcag cgccactggc acagccacga ggacgagctc
     1921 gtctacgtcg tgagcggcga gctcgtgctt gtaaccgacg ccggcgaaac ccggatgcgc
     1981 gccggtatgg tcgcgggctt tcccgccggt cgccaggacg gccatcacct gatcaaccgc
     2041 tcggacgcgc ccgcgaccta cctcgagatc ggcagccgca tccccgcgga cggggcagtc
     2101 tattccgaca tcgacatgga agtccgcccc cggcccgagg gcggccacgt cttcgtccac
     2161 aagagcggcg agccatattg aagcggcgct cgtcgtttgc tgaaccgttg acgcccggac
     2221 ctgcggggcg catccttcaa ccatggcgat aagcgccatc aggtcgcgtc acggcggtcc
     2281 gccggaggcg atcggtcagg gaggcgacaa tggcagacca accggcagag gacggcgcgt
     2341 cgctgtatga gcggttaggt ggcgtctaca gcatcgcgac cgtcgtggac gatttcatcg
     2401 atcgaatcat ggtcgatccc cggctcaatg ccaatccgcg ggtcgacgaa gcgcaccacc
     2461 gcgtgccgcc ggccggtttc aaatacctgg tgaccgagat gctctgctgg gccgcgggcg
     2521 ggccgcaacg ctacaccggc cgggacatgg aggaaatgca ccgccatctg ctgatcacag
     2581 cggacgagtg gcaggccttc atggacgatc tgcaacagac gctcgacaag ttcagggtgc
     2641 cggcagccga gcaggcggag gtcagagcca tcgtcgacag cacccggagc ggaatcgtgg
     2701 tgccgaaggc cgccccggag atcgtccggg gcagcgacac tgtcggccaa ggactccggg
     2761 catgacaacc gtcggccagg tcgagagcct ctggcgctat ccggtcaaga gcatgcgcgg
     2821 cgaggagctg gccgaagcgt tcatcggctt tgccggcgtc tacggcgacc gtctgtttgc
     2881 cttcaagagc tcaagccggc cggcgggttt cccctatttg accggacgcg agcaggaatc
     2941 gatgctgctc tatcggccgc ggttccgcga tcctgacaag gctgcgaagc cgcccaatct
     3001 agcggaagcc gaggcgatcg cgcccggcat cacgccacta cccggtgctc cggccgacct
     3061 cgtggtcgag gtcgagaccc cgagcggcga ggtgctggcg gtcgatgacc ctgccttgac
     3121 ccgcaggctc ggggagggca gccccgacgg ccgggagctc actttgctgc gctcggagcg
     3181 ggcgatgacc gattgccggc cggtctcgct gttctcgatc cagaccgcgc gccagctgac
     3241 gcaggagacc ggcatcgcgc tcgacaagcg acgcttccgc gccaatgtct acctggacct
     3301 tcgcgatgcg cagggttttg tcgaggatgg ctttgtcggt cgccgcctgc gactcggacg
     3361 gaaggcggtg atcgcggtgc tcgaacgcga cccgcgctgt aagatgatca cgctcgatcc
     3421 cgaaacggcg aagccaattc ccgatatcct ggccaaggtc acgcgcgtcc accagggtat
     3481 ggcaggggta tacgccgccg tcctggtcga agggacggtg cgccagggcg acgatgtcga
     3541 cctcatctga gcgcggattc cggtcatgcc gctcggaact ggcgtcaact tcgtaagagg
     3601 accgcccgga cttgtccttt tcgacggttg caactcgggc gccaacgccg tcgcattctc
     3661 ctcgtgacca cggcgtactg gcgaggaccc tcaccatgta cggcatggtc aatctcgcga
     3721 ttcaagagtt tctctgtgac cgctatggcc acgatcggtg gacggcgatc cgagccacga
     3781 tccccaagga aactgaccgc ttcctaacta tggagcaata ccccgatcaa ctttcctttg
     3841 acattatgac agcggcctcc gacctcatcg ccaagccgat ttctcagctc ctcgaagaac
     3901 tcggagagta ttggattgca tttgcgcagc gctccggcta tggcgacctg atggacgttc
     3961 tcggcgagga tttgtttgaa gcgttgcaaa atctggacaa cttgcatacc cgagtcggct
     4021 ttgcctttcc cgatctgcga cctccatcat tctggctgac cgacatcgaa gatgacacgc
     4081 tcatactaca ctatcaatcc gtaagatcgg gactcgctcc aatggtaatt ggctggtgcg
     4141 cggactggca cgccgcttca accaaaatgt cagcgtacgg caaatcgagc cgactggccg
     4201 cgatcacagt ttattcaaag ttacgattgt acaatctgac aaagattgtt gataagtctg
     4261 agttacatcc gcgaacggga ctcatcatca tgtacggact gaactttcgc caatttgcgc
     4321 gaatacatcc gtttcatttg gtgatcgatg cagatatgca tatcgttcaa gcgggaaccg
     4381 cgctgcgaaa gatatttaca gatctaatcg gtaagaaatt ctatcaacac tttgagattg
     4441 tccgaccccg cctaactctc cgctttgaga cgattcgcga ggcgggcaat gccgcatttt
     4501 tacttggttg ccccagcctt cagatgaacc tgaaaggaca gatgctggcg gacgagtcga
     4561 gaagattgct tttcttcctc ggcgcaccac ttatcgaaag cgcgacactg cttcgtcggc
     4621 atcatttgac cttaactgat tttccgatgg tggactcttc aatagatatt ctcgtttcgc
     4681 aacaacaaca actcgtgaat aaaaaactcg aaaaccttat acttgagcga acagaagatc
     4741 ttgagaaaga acgaaagata gctgagagat tgttgcatgc gatactgccc gcacacatcg
     4801 tcggtaattt gaagaaccga ccaagcgcca tagcggaaca gtttgaacag gtgaccgtta
     4861 tgtttcttga cctggttggt ttcacgcctt tgacggcgaa tatgccggcg atcgcggtgg
     4921 ttaacttgct gaatggaatt tttcagaggt tcgacgagct ggtcgaagcg acggacgtcg
     4981 agaagatcaa gacgattggg gatgcataca tgtgcgtcgg cggtttgagt ggggcgtcgg
     5041 cgcagcagtc ggccgagcga gtggcgctgg cgggcttgca gatgctgcgg atcatcgacg
     5101 agaccgtgcg ggcggagcgc ttgcaggcgc ggatcgggat gcacaccggc ccggcggtgg
     5161 cgggggtgat cggagccacc aagtttgcgt acgatttatg gggggatacc gtgaacacgg
     5221 cagctcgtat ggaatcgcat ggcgaaccgg ggaagatcca ttgcacgcgt gaggtgtatc
     5281 agttgctggg cgagaacttt gagtttgagg ctcggggtga gcatcatata cgggggaaag
     5341 gccggatgca gacgtatttc ctgctgggcg agaaaaatga tcgggcggga gcggattgac
     5401 aggaacggca ccgcgtctcg ggtgtgcgac cgcgcagccc atggacgatc cggtggtcgg
     5461 gcgatccggc cgatctgact gggcagggtc aacgacgatc agcgagagcg ggagcgcgag
     5521 ctaccggccg gtcgtgaaga ccaggctcac cggtgtgggg acctcggtga tgttgccggt
     5581 cggcgtgagt tttccggtct tttgatcgat cgcaagttgt acgatgctgg cgcccttctg
     5641 attgcagaca tagagccatt ttccgctcgg gtcgagctgg aaggcgcgcg ggaaggcgat
     5701 gttctcggt
//
LOCUS       DATAOZ010000175         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8755537, whole genome shotgun sequence.
ACCESSION   DATAOZ010000175 DATAOZ010000000
VERSION     DATAOZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8755537"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1312)
                     /locus_tag="VHR64_04750"
     CDS             complement(125..1312)
                     /locus_tag="VHR64_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX3302168.1"
                     /translation="MPLACLLIPHFSLRLAVLDQPALDGLPLVLTSPSMSRPLVADAS
                     PEAVERGIRVGMPLREVNAVCAQAVFIPANPVRDALAFEQILAQLESVSPALELVEPG
                     RCYIDIAGLDRHYPSPEAIAEALLACVSPILRPRAGLAPTRFSAWVAARMASPGTVRT
                     IQPADLAATLGPLPVSWLPLEPNTLLKLDRLGLRTLAEIRELPASALQARFGPAGREA
                     HACASGQDEARVYPRKLVESVREEFRLPAPMASREMLMLAIRQLVIRAFNRPALKHRQ
                     IRQSRIRIAIEGGQSWERELTFREPVGSQRLIETLRSRLQAIELPGPAEMIHLDLIGI
                     VNEIATQELIPLLRPKRDRPLIEAARQLKQRYGESPLAHIVEVEPWSRIPERRFALIS
                     YDP"
BASE COUNT          282 a          478 c          552 g          248 t
ORIGIN      
        1 cgccaccact catcgttgag ctgccagcga tcctggaccc gctcgaccgg ctcatgtcgt
       61 tttccttcga tgatcgcgat cggaacgccg cggtcggtct ggaccgtaca gcggcgggga
      121 cgattcaggg gtcgtagctg atcagggcga atcgacgttc cggaatgcgc gaccatggct
      181 ctacctccac gatgtgagca agcggggact ccccatatcg ttgtttcagc tggcgagccg
      241 cctcgatcag cggacgatcc cgtttcggcc gaaggagcgg gatcagctcc tgggtcgcga
      301 tctcgttgac gatgccgatc aggtcgaggt ggatcatctc ggccggaccg ggcagctcga
      361 tcgcctgcaa ccggctgcgc agggtctcga tcagccgctg gctgccgacc ggctcgcgga
      421 aggtcagctc ccgctcccac gactgcccgc cctcgatcgc gatccggatc cgggattggc
      481 ggatctggcg gtgcttgaga gcagggcggt tgaaggcgcg gattacgagt tggcggatcg
      541 cgagcatcag catctcgcgg ctggccatcg gcgccggcaa gcggaattcc tcgcgcactg
      601 actcgaccag cttgcgcgga tagacccggg cctcatcctg tccggaggcg caggcgtggg
      661 cttcgcggcc ggccggtccg aaccgggctt gcagggcgga ggccggcaac tcgcgaatct
      721 cggccagggt gcgaagcccg agccggtcca gcttcaggag cgtgttcggc tcgagcggca
      781 gccaggagac cggaagcgga ccaagcgtcg ccgcaagatc ggccggctgg atcgtccgga
      841 ccgtccccgg agatgccatc cgggccgcaa cccaggccga gaagcgggtc ggagccaagc
      901 cggcgcgcgg ccgcaggatc ggcgagacgc acgccagcag tgcctcggcg atcgcttcgg
      961 gcgacgggta atggcgatcg agcccggcga tatcgatgta acagcggccg ggctcgacca
     1021 gttccagggc cgggctgacc gactcgagct gggccaggat ctgctcgaag gcgagcgcat
     1081 cccggaccgg attggccgga atgaagacgg cctgcgcgca gacggcattg acctcgcgga
     1141 gcggcatccc gacccggatc ccgcgctcga ccgcctccgg ggaggcatcg gcgacgaggg
     1201 gtcggctcat ggatggtgac gtcagaacga gggggaggcc gtcgagcgcg ggctggtcga
     1261 gaacagccag gcgcagggaa aaatggggga tgagcaggca cgcgaggggc atcggtggct
     1321 cccgggtgtg acaaaacgga acacttgttc tattgccagg acgcagttat gctgcctgta
     1381 cccacatcga aaccttgggc agacataggc actatagaac atatgttcta attacgcaat
     1441 aggggttttc ggttttggaa ggagcgtacc agtcaggcgg gtacgacgcg acgatcggca
     1501 ggtgcatcgc cacgacttgt agggatttgc cgtggcaaat ccgcgccagg atatcgtccc
//
LOCUS       DATAOZ010000176         5506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7966283, whole genome shotgun sequence.
ACCESSION   DATAOZ010000176 DATAOZ010000000
VERSION     DATAOZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7966283"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..752
                     /locus_tag="VHR64_04755"
     CDS             270..752
                     /locus_tag="VHR64_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302169.1"
                     /translation="MFRIIPTLWKGALAALIAVVIGLSASAGPAGATEVPSVQITVTD
                     GGYAVRPEVAAGQYQVTIENLSSQGVDLLLVRIPDGMSPAEVQAVLVTGTGKLGCGAI
                     EQSVIEAAQAADVISLGQAAPHGQGRTTVTLETGTWVVMSGSAGTDSTFAEFNVGSAS
                     "
     gene            879..1004
                     /locus_tag="VHR64_04760"
     CDS             879..1004
                     /locus_tag="VHR64_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302170.1"
                     /translation="MVAATQIERTPLDYATWHARIKGHNDSGAPAATNMPDARTA"
     gene            1048..1290
                     /locus_tag="VHR64_04765"
     CDS             1048..1290
                     /locus_tag="VHR64_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302171.1"
                     /translation="MIPTIDIEWMVIKESLGVEEAARWRRARVEEMLGMTSNPEAPKP
                     TRDGRAFAIGSWLTRAWTTVVGCRLRWSTPQVACQA"
     gene            1397..2257
                     /locus_tag="VHR64_04770"
     CDS             1397..2257
                     /locus_tag="VHR64_04770"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3302172.1"
                     /translation="MNESLRLMALLAHPDDESLGFGGTLAKYAAEGVHVALVVATRGE
                     RGWTGEEDANPGIAALGQIREAELRAAATALGIDRLTFLDQIDGELAKTESGLMLARI
                     VAEIRAFRPQVVITFSPDGAYGHPDHIAISQLTAAAIVLAADPAYPVIGNVEPVQVRK
                     LYYRVWTAAEHVPFREVFGPVEMEIEGAIRKWVAWPDWSVTTTIDTADYWSEVQEAVL
                     SHQSQLGAVPRLAELDQRDLRILWGTQQYYRVFSTVPIENGLEADLFAGLRIPTESTG
                     VSDPAEIVLI"
     gene            2450..3778
                     /locus_tag="VHR64_04775"
     CDS             2450..3778
                     /locus_tag="VHR64_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEX3302173.1"
                     /translation="MDIVLSAGAVGSVRPHRSFIRDINSANIAAGLTAGLFYMFGAIP
                     LFLSAAQRLNLSAAETTSWFFITFMTSAVASLVISLVLRTPLPIGWSMPGLVFLATMG
                     DRYSHGEMVGAFMAAGVLMIALGALGVGEQIMRLLPLPIVMGMFAGNVLAYVTGVFTS
                     LDRQPWVVGAAIAGYLASRALGRVWLPPMFGAVLAGLIAAGASGQINTGALEWSAPTV
                     APVRPEFDIGSLAALTIPLVVMAIGMGNVQGLGILVAQGYRPPVAKMTIAMGLTTLIN
                     ATFGGHVSSIQNNGAAILGGPDAGPREQRYFASVMASVIAAVLALCAATAGSLLGLLP
                     AGLVPALAGLALMSSMMDALQKATRTDLSMGAFFALVIAASGLTVLGIGPAFWALVGG
                     MVASLMLERPSLVAAWRAGRPPERLATAQPERTATPDLPCGPFSPQLACP"
     gene            3832..5436
                     /locus_tag="VHR64_04780"
     CDS             3832..5436
                     /locus_tag="VHR64_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3302174.1"
                     /translation="MRPQFGYARSGEVSIAYQVVGNGPIDLVFVMGWVSHLDAYWEEP
                     RFARFLERLASFSRVILFDKRGTGLSDAVPIDALPTLEQRMDDVRAVMDAVGSTRAAL
                     LGASEGAPMSALFAATYPDRCAALVMVGGYARRLWAPDYPWAPTMDKRLGYIEDIQRG
                     WGGPVGLDRLAPSLADDEPFRAWWSSYLRRGASPGAAVALAHMNMEIDIRKMLHAIHV
                     PTLVIHRTDDIACPVGGGRYMAEHIPGARFVELPGCDHLVFVGNQDEVIGEVEQFLTG
                     VRRIPADEPDRVLATVMATEIVDAAQTATRLGDVRWRDVLGAYDRVVRDAFSRHRARR
                     ESQMMDGWIALFDGPARAVRCAREIVEGGRKLGLRVRAGLHTGEVLETEAEVVGVAMH
                     LATRVRSHAAPGSVLVSQTVKDLVAGSGLAFDLVGDQVFAGLPGEWNLYEVADGSAGE
                     RIAPVTLPGAGSRLAVLSPREREVAALVALGLSNRQIADELSISPATVERHVANMLTK
                     LGHRSRAQIAAWATEQGLSRTAAEQS"
BASE COUNT          910 a         1666 c         1904 g         1026 t
ORIGIN      
        1 ggatcattac ttcgggtctt tcgattccgc gcgacggcgt ctatggaggt cggggtacga
       61 gcatcacggc ctccttgcgg tatttcgcgg accttcacct aaatgcctgc aattgcatgt
      121 ccctgtacat gttcttcgtg atgttttgag gtgtccccgt ccataggctg aggatgggta
      181 gctcggacgt gaccggtatt cggactggcc gtcgagcgac ctggaatcgg ccgcgggacg
      241 tcagtgccgc ggcggaaggg gacagttcga tgtttcgaat aattccaact ctctggaaag
      301 gcgcgttggc ggcgctgatt gcggtcgtca tcgggctctc agcgtctgcc ggcccggccg
      361 gggcgaccga ggtcccgtcg gtgcagatca cggtcacgga cggcgggtac gcggtgcggc
      421 cggaggtcgc ggccggtcag taccaggtca cgatcgagaa cctgagcagt cagggtgtcg
      481 acttgctgct cgtccgcatc ccggacggca tgtcgcccgc ggaagtgcag gcggtcctcg
      541 tcacgggtac cggcaagctg ggctgcgggg cgatcgagca atcggtcatc gaggcggctc
      601 aagccgcgga cgtgatctct ctcggccagg cagccccgca cggccagggc agaacgaccg
      661 tcacgctcga aacaggcacg tgggtcgtga tgtccgggag cgccgggacc gacagcacgt
      721 tcgccgaatt caatgttgga tcagccagct aagcggccga tggcacgtca cgaatggcgt
      781 gacgcagttt gacttcaaat gaagaaagta cgaggcaaga aatgtatcgg tacatgaata
      841 cgacaggacc acggtttagc cgaaatggac ccagtgccgt ggtggccgca actcaaatcg
      901 aacgcactcc gctcgattac gcgacctggc acgcccggat caagggccat aacgacagtg
      961 gcgcaccggc agcgaccaac atgccggacg cgcgaacagc gtagcgcggc cgccttcgaa
     1021 tcacatcagg ccgaaaggga acaacacgtg ataccgacga tagatattga gtggatggtg
     1081 atcaaggaaa gtctgggggt cgaggaagca gccaggtggc ggcgagcccg ggtcgaagag
     1141 atgctgggca tgacctcgaa tcctgaggcg ccaaaaccga cccgggacgg acgagccttc
     1201 gcgatcggct catggctcac gcgagcctgg accaccgtcg ttggttgtcg cctacgctgg
     1261 tcgacccccc aggtcgcgtg tcaggcgtaa ctgccggtag aggacttcac tcaatcctgg
     1321 gcgcgttttc gggcgttgac atcgttgccg atccggaagc gcgccgggat gagcaggagc
     1381 gatcgggagg atgcaaatga acgagtcgtt gcgattgatg gcattgctgg cccacccgga
     1441 tgacgagtcg ctcgggttcg gcgggacact cgcgaagtac gcggccgagg gcgtccatgt
     1501 ggcactggtc gtggcaacgc ggggagagcg cgggtggacc ggcgaggaag atgccaatcc
     1561 ggggatagcc gcgcttgggc agatccgcga ggcggagctt cgcgccgccg cgaccgcgct
     1621 ggggatcgat cgtctgacgt tcctcgacca gatcgacggc gagctggcca agaccgagag
     1681 cggtctcatg ctggcccgga tcgttgccga gatcagggca tttcggccac aggtggtcat
     1741 cacgttcagt ccggatggcg cctacgggca cccggatcac atcgcgattt cgcagttgac
     1801 ggcggcggcg atcgtgcttg cggccgaccc ggcgtacccg gtcatcggca atgtcgagcc
     1861 ggtacaggtg cggaagctct actaccgcgt ctggaccgcg gccgaacacg ttccgtttcg
     1921 cgaggtattt ggccccgtcg agatggaaat cgaaggggcg atccggaaat gggttgcctg
     1981 gccggactgg tcagtcacca ccaccatcga cacagcggac tactggtcgg aagtgcagga
     2041 ggccgtgctg tctcatcaga gccagctcgg cgcagtcccg cgcctcgccg agctggacca
     2101 gcgtgatctt cgcatcttgt ggggaaccca gcagtactac cgcgtcttca gcaccgtgcc
     2161 gatcgagaac ggtctcgaag ctgacctgtt cgcgggattg cggattccga ccgagtcgac
     2221 cggcgtatct gacccagcgg aaatcgtgtt gatttgaaat ggtccgaagg ctcgttctgg
     2281 agccatgtca tgaaaaggaa ggcgcgtaat ggacttgttg atcttgattg ggcttctcat
     2341 cgtactcgga atcctggcga atgtcgccgg gactgacagc cgggacacac tcgctggcta
     2401 gcgccgcatg cgaggtcctg accaactgaa tatatgaagg aatacatcga tggatattgt
     2461 cttgagcgct ggtgcggttg gctctgtccg accgcaccgt tcattcatac gagatatcaa
     2521 cagtgccaat atcgcggccg gcctgacggc agggctcttt tacatgttcg gggcgatccc
     2581 gctcttcctg agcgccgccc agcggctgaa tctctccgcc gccgagacga cgagctggtt
     2641 cttcatcacc ttcatgacct cagcagtggc cagcctggtg atctcgctcg tgctccgcac
     2701 gcccctgccg atcggctgga gcatgccggg gctcgtgttc ctggcgacga tgggagaccg
     2761 ctattcgcat ggcgagatgg tcggtgcgtt catggccgcc ggagtgctga tgatcgccct
     2821 gggtgcactt ggcgtcggcg agcagatcat gcggttgcta ccgttgccga tcgtgatggg
     2881 gatgttcgcc ggcaatgtgc tggcttacgt gaccggggtc ttcacatcgc tcgatcgcca
     2941 gccctgggtt gtcggagccg cgatcgccgg ttacctggca tcccgcgccc ttggtcgagt
     3001 ctggttgccg ccgatgttcg gtgcggtgct ggccggactg atcgcagcgg gagcgagcgg
     3061 ccagatcaat accggcgccc tggaatggag cgcaccgacc gtggccccgg ttcggcccga
     3121 gttcgatatc gggagcctgg ccgcgctgac gattccgctc gtcgtaatgg cgatcgggat
     3181 gggcaacgtc cagggactcg ggattctcgt cgcgcagggg tatcgcccgc cggtggcgaa
     3241 gatgacgatc gcgatgggcc tgaccacact gatcaacgcg accttcggtg ggcacgtctc
     3301 gtcgatccag aacaacggcg cggcgatcct gggcgggccc gatgccggtc cgcgcgagca
     3361 gcggtacttc gccagcgtga tggcgtcggt gatcgcggcc gtgctggcgt tgtgcgcggc
     3421 gacggcgggc tcgctccttg gcttgttgcc ggccgggctg gttccggcgc tggccggatt
     3481 ggcgctgatg agttcgatga tggacgcgtt gcagaaagcg actcggactg atctgtcgat
     3541 gggcgcgttc ttcgccctcg tgatcgcggc ttccggcttg accgtcctgg gcatcggccc
     3601 ggcattctgg gcgctcgtcg gcggtatggt cgcgtcgctg atgctggagc ggccatcact
     3661 ggttgcggcg tggcgcgccg gcaggccacc tgaaaggctg gcaacagctc agcctgagcg
     3721 gaccgcgacg ccggacctcc cgtgcggccc gttttcaccc cagctcgcgt gcccttgaca
     3781 tatcttgcgg ctgtccgaaa agattgggga cgtaccacga aggcgaaggc catgcgtccc
     3841 cagttcggat atgcgcggag cggcgaggtc agcattgcct accaggtcgt cgggaacggc
     3901 ccgatcgatc tggtcttcgt gatgggctgg gtctcgcacc tcgacgccta ctgggaggag
     3961 ccgcggttcg cccggttcct cgagcgtctg gccagcttct cgcgggtcat cctgttcgac
     4021 aagcgcggca ccgggctctc ggatgccgtg ccgatcgatg ccctgccgac gctcgagcaa
     4081 cgcatggacg acgtgcgcgc cgtcatggac gccgtcggat ccacccgcgc ggccttgctt
     4141 ggcgcgtcgg agggcgcgcc gatgagcgcg ctctttgccg cgacctaccc ggatcgctgc
     4201 gctgcgctgg tcatggtcgg gggatatgcg cgtcgacttt gggcgccaga ttatccgtgg
     4261 gcgccgacca tggataagcg actcggctat atcgaggaca tccagcgcgg gtggggagga
     4321 cctgtcggcc tcgaccgcct ggcgccgtca ctcgcggacg acgagccgtt ccgggcgtgg
     4381 tggtcatcct atctgcgccg cggggcgagt cccggcgcgg cggtcgcgct ggcccacatg
     4441 aacatggaga tcgatatccg gaaaatgctc catgcgatcc atgtcccgac cctggtcatt
     4501 caccgcacgg atgacattgc ctgcccagtc ggcggcggtc ggtatatggc cgagcacatc
     4561 ccgggcgcgc gcttcgtgga attgccgggt tgcgatcatc tcgtgttcgt ggggaaccag
     4621 gacgaggtga tcggggaggt cgagcaattt ctgaccggtg tgcgccggat ccctgccgat
     4681 gagccggatc gcgtgctggc gacggtcatg gcaaccgaga tcgttgacgc ggcccagaca
     4741 gcgacgcggc ttggcgatgt gcgctggcgt gatgtcctgg gagcttacga tcgcgtcgtg
     4801 cgcgacgcct tctctcggca ccgggcacgc cgggaatcgc agatgatgga cggctggatt
     4861 gccctgttcg atggcccggc ccgggcggtg cgctgcgcgc gggagatcgt cgagggcggc
     4921 cggaagctcg gattgcgggt gcgtgccggt ctgcataccg gagaggtact ggagaccgag
     4981 gccgaggtgg tcggcgtggc gatgcacctt gcgacgcggg tgcgaagtca cgccgctccg
     5041 gggtcggtgc tcgtctcgca gaccgtcaag gatctcgtcg ctggttccgg cttggcgttc
     5101 gacctcgtag gggatcaggt atttgccggg ctgcccggcg agtggaacct ctacgaagtg
     5161 gcagacgggt ccgccggcga gcgaatcgcg cccgtgacct tgcccggtgc cggatcccgg
     5221 ttggcggtgc tcagcccgcg ggagcgcgaa gttgccgctc tggtcgcgct cggtctgtcc
     5281 aaccgccaga tcgcggatga gctctcgatc tccccggcca cggttgagcg ccacgtggcg
     5341 aacatgctga cgaaactcgg tcaccgctcc cgcgcgcaga tcgccgcctg ggcgaccgag
     5401 caagggctgt ccaggaccgc cgcggagcag tcctgacccc ccacatcgct gaccccgtca
     5461 acttacatgg tcctgtacat ggtctcgatg atgtgcggcc gatccc
//
LOCUS       DATAOZ010000177         5624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8755966, whole genome shotgun sequence.
ACCESSION   DATAOZ010000177 DATAOZ010000000
VERSION     DATAOZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8755966"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..785
                     /locus_tag="VHR64_04785"
     CDS             273..785
                     /locus_tag="VHR64_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX3302175.1"
                     /translation="MFEAIHPFSDGNGRTGRLLISLMLREAGLLQEPLLHLSRYFEQH
                     KERYLNGLYEVSLRGDWHGWVEFFLIAVATEASRAVDTVRKLLAMRDDYRTRYRRHRA
                     KRLSEVTDLLFSQPVQSVSSIAEALDMNYKTARSMVGILAADGILEETTGKQCNRIYL
                     APEIMQLLEE"
     gene            911..2467
                     /locus_tag="VHR64_04790"
     CDS             911..2467
                     /locus_tag="VHR64_04790"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3302176.1"
                     /translation="MAVPAHAPEEARPVLLRASGIEKRYGGIVALRGVDLEIAPGEVH
                     ALMGENGAGKSTLAKIIAGVQTADAGTIEWQGHPVTYRATQEARDAGIAIVLQELNLI
                     PDLSVAENIFLTHPESYAGGLFLKRRDINQRTHELFDRLGWELPINPAQKVSELSVAE
                     QQMVEILRALSWEAQLYILDEPTATLSSREVEVLFGMIRRLKEQGISFLLVTHRIEEV
                     FALSDRITVYRDGANSGEFVTSQTTEQELIRAMVGRDLGDFFGVRTRREPGEPILTVT
                     NLCRGQRLRNCSLTVRRGEVVGIAGLVGAGRTELIRAIFGADKADRGEIQLRGRAGLI
                     GNPSQAINESTAMVPEDRKAHGLLIDLPIQHNVAMVGLTTRRGFWLRKRAERDLMTRM
                     TERLQIRANDPKKPASSLSGGNQQKIVLAKWLAIDPDLLILDEPTRGIDIGTKYEIYK
                     LIDGLVEQGKAVLLVSSELPEILALSDRILVMRDGELVGELAHGQADEASILALAAQG
                     SLIAERAEVA"
     gene            2470..3456
                     /locus_tag="VHR64_04795"
     CDS             2470..3456
                     /locus_tag="VHR64_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302177.1"
                     /translation="MAQAATASIGQPSRRGQTRLIDEYRVELAMFGVLILLGAVLTIF
                     ADNFLTERNMRNVMWQSTAIGIVAIGQTLVILTAGIDLSVGGMAALSAMVGGKVMMET
                     NVAVGIIVTLAVATFIGTVNGVFVAYGKLAPFIVTLGVLSICTSLTYVISDQASIVGL
                     PDSYRQLGRGKIGSYQYYIFIFFILYILGHIMLTRTKPGRFIYAIGSNEEAARLSGVN
                     IRLFKSFAYVMSGLLAGIATLILSGRLGVIDPDSGSGWELQSIASVVIGGTSLFGGKG
                     SLVGTMIGVFLIAVLNNGLNLLRVSPFWQGSAVGAVVILSVLLERIFRNRGA"
     gene            3810..4877
                     /locus_tag="VHR64_04800"
     CDS             3810..4877
                     /locus_tag="VHR64_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3302178.1"
                     /translation="MTSKAVTSVLTTPLNRRETIKRAAAVGLAVPSILTLPKITRAQQ
                     TIHIAYITPGLNVPFWKYLSDGIKQKAAQLSSENGVDIQVTDYDSRNSAQTQLQNAQD
                     VVTAGVDAILISPTDSSTCPAVLEVAAEANVPVIISDIGTDSGDYVSFVISTNEQGAY
                     DAGQVLIKEMDAKGWKGADVLMITISQARQNGKNRTKGVTRAVEEGGSKIVQFLQSEQ
                     YTRAEAQGQALDLMTANPDAHGFFTQHDEATLGTWAAIEDAGNTDNIILVGFDGSPES
                     VELIRQGKLRGASMQQPVLMGRDSMDVAWRHLQGQDVEKETSVPTILVTPDNLKDTEK
                     TLQDTVFPVEGGATPGASPTS"
     gene            complement(4962..5534)
                     /locus_tag="VHR64_04805"
     CDS             complement(4962..5534)
                     /locus_tag="VHR64_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302179.1"
                     /translation="MRTGKTAATVIFSRVMMAGAFALILGLMLAQGVAARPAKGVSVS
                     DRVQAQSDLCMVSGGTFESRDYYGSYTNGHQFIKNVTTCTGGETPQTCTNTKNDTTCK
                     ASLVIQPKGPLDGIAVDESQISGVDPGPAPQDPLDGSSVDESQIGGVQQSSEPAPIDP
                     VSTGGTITVPVRVDRSSLDGSNLSPIDPLP"
BASE COUNT         1036 a         1802 c         1786 g         1000 t
ORIGIN      
        1 cgccgtcggc cgcgaaccag atctcgatct catgcggccg tccggaggcc cgcccgatcg
       61 tctcgaggta gcaaaccgac gcattgattt gggacggagc aagcgtagcc atgcgcagat
      121 catcctccgg catccgccat cgcacaactg cccaagcggc gaggccagca gcgacgtatt
      181 gttattgcat ctcatgcaat aagggtcccc gctatttcat ttcgcgggtc gaagtgaaac
      241 ggcgagtccc ctaacgcaca cattgtcccg gaattttcga agccatccac ccattcagtg
      301 atggcaacgg tcgcaccggc cggttgctca tctccttgat gctgcgcgag gccggcctcc
      361 ttcaggagcc gctgcttcat ctctcgcgct atttcgagca gcataaggag cggtacctca
      421 acgggcttta cgaagtcagc ctccgcggcg actggcatgg atgggtggag ttcttcttga
      481 ttgcagtcgc gacggaggca agccgcgcag tcgacacagt ccggaagttg ctcgccatgc
      541 gggatgacta ccggacgagg taccggcgcc accgagccaa gcgactgagc gaggtgaccg
      601 atttgctctt ttcacagccc gtgcaatccg tgtccagcat cgcggaggca ctggacatga
      661 attacaagac ggcccggagc atggtgggga tcctggcggc cgacggcatc ctcgaggaga
      721 cgaccgggaa gcagtgcaac cggatctacc tggctcccga aatcatgcaa ttgctggagg
      781 aatgacgcgg gacgtggctt cgtaggatgc atgcccgctt gattccctga attcatataa
      841 tgaactccag atcgttgcca ccgagtcatc cgccgggtgc ggccggaatt ggctcgacca
      901 ggagttgtcc atggctgtcc cggcccatgc cccggaggag gcccgcccgg tcctcctccg
      961 cgcatcaggc atcgagaagc gctacggcgg catcgtcgcc ctccgcgggg tcgacctcga
     1021 gatcgcgccc ggcgaggttc acgccctgat gggcgaaaac ggcgcgggca aatccaccct
     1081 cgccaagatc atcgccggcg ttcagaccgc cgatgccgga accatcgagt ggcaggggca
     1141 tccggtcacc taccgggcca cgcaggaagc ccgggacgcc gggatcgcga tcgttctcca
     1201 ggagctgaat ctgataccgg atctctcggt cgccgagaac atcttcctga cacatcccga
     1261 gagctacgcc ggcggcctct tcctcaagcg gcgcgacatc aaccagcgca cccacgagct
     1321 atttgaccgg ctcggctggg agttgccgat caacccggcc cagaaggtca gcgagctgtc
     1381 ggtcgccgag cagcagatgg tcgagatcct gcgtgccctt tcgtgggagg cgcagctcta
     1441 cattctcgac gagccgaccg cgacgctcag cagtcgcgag gtcgaggtcc tcttcggcat
     1501 gatccgccgc ctcaaggagc aggggatcag cttcctgctg gtcacgcacc gcatcgagga
     1561 agtcttcgcc ctcagcgacc ggatcaccgt ctaccgggac ggcgccaact ccggcgagtt
     1621 tgtgaccagc caaacgacag agcaggaatt gatccgggcg atggtcgggc gcgacctcgg
     1681 cgacttcttc ggcgtccgca cccgtcgcga gccgggcgag ccgatcctga ccgttaccaa
     1741 tctctgccgg ggtcagcgtt tgcgcaattg ctcgctgacc gttcggcgcg gcgaggtcgt
     1801 cggcatcgct ggtctggtcg gtgccgggcg gacggagctg atccgggcca tcttcggcgc
     1861 ggacaaggcc gatcgcggcg agatccagct ccgcggccgg gccgggctca tcggcaaccc
     1921 gagccaggcg atcaacgagt cgaccgcgat ggtcccggag gatcgcaagg cccacggact
     1981 gctgatcgat ctgccgatcc agcacaacgt cgccatggtc gggctaacga cccggcgcgg
     2041 tttctggtta cgcaaacggg ccgaacgcga tttgatgacg cgcatgaccg agcgcctcca
     2101 gatccgggcc aacgacccca agaagccagc cagcagtctc agcggcggta accagcagaa
     2161 gatcgtgctg gccaagtggc tggcgatcga cccggacctg ctgatcctcg atgagccgac
     2221 ccggggcatc gatatcggta cgaagtacga gatttacaag ctgatcgacg gtctcgtcga
     2281 gcaaggcaag gcggtcctgc tggtctcttc ggaactgccg gagatcctcg ccctctccga
     2341 tcgcatcctg gtcatgcgag acggcgagct ggtgggagag ctggcgcatg gccaggcgga
     2401 tgaggcgtcg atcctggcac tggcggcgca gggatcgttg atcgccgaac gagcggaggt
     2461 agcgtaggca tggcgcaggc agcaacagcg tcgatcgggc aaccgtcccg gcgcgggcaa
     2521 acccgattga tcgacgagta ccgcgtcgag ctggcaatgt tcggcgtcct gatcctgctc
     2581 ggggccgtcc tgacgatctt cgccgacaac ttcctgaccg agcgcaacat gcgcaacgtc
     2641 atgtggcagt cgaccgcgat cggcatcgtc gcgatcgggc agacgctggt gatcctgacc
     2701 gccgggatcg atctctcggt gggcggcatg gcggcgcttt cggcaatggt cggcggcaag
     2761 gtcatgatgg agaccaacgt cgcggtgggc atcatcgtga cgctcgcggt ggccaccttc
     2821 atcggcaccg tcaacggagt attcgtggcc tatggcaagc tggcgccctt catcgtaacc
     2881 ctgggcgtgc tctcgatctg cacctcgctg acctatgtga tctcggacca ggcctcgatc
     2941 gtgggcctgc cggacagcta ccggcagctc gggcgcggca agatcggctc gtaccagtac
     3001 tacatcttca tcttcttcat cctctatatc ctcggtcaca tcatgctgac ccgcaccaaa
     3061 ccggggcgct tcatctacgc gatcggctcc aacgaggagg cggcccggct ctcgggtgtc
     3121 aacatccgcc tcttcaagtc attcgcctac gtgatgtccg ggctgctggc cgggatcgca
     3181 acgctgatcc tctccggccg actgggcgtg atcgacccgg atagcggcag cggctgggag
     3241 ctgcaatcga tcgccagcgt cgtgatcggc ggcacgagcc tgttcggcgg caagggcagc
     3301 ctggtcggga cgatgatcgg cgtcttcctg attgcggtgc tgaacaacgg gctgaacctg
     3361 ctccgggtct cgcccttctg gcaaggctcg gcggtcggtg cggtcgtgat cctgtcagtc
     3421 cttctggagc ggatcttccg caatcgaggc gcgtaggcgc ggaggcgcga agttacacgt
     3481 cgtagtggcg gggctctgta cccgcctaga ggtgacggcc gcatcgttcc cctgcactgc
     3541 gcggctcata ccaaaccgtt cggcgtgccg gctcggttgt ggcgtggtcg gctccgtcaa
     3601 tcgggtcgtg tggcagatca aggcgggtac ggagccccac cactacgaaa tgacgctggt
     3661 cagccggctt tagccgggtt caggtttcag ccccggtctt caggccgggc gacagtgtcg
     3721 gaaaggaggc agcgctaatc cgagataaca gattcgttgg tgatggtcag tcgagtgagg
     3781 ctggccgggg ttacgagaaa gggaaccacg tgacaagcaa ggctgtgaca tccgtgttga
     3841 ccaccccgtt gaatcgacgt gagaccatca agcgcgccgc cgctgtggga ctggctgtcc
     3901 cgtcgatcct gaccctgccg aagatcacgc gcgcccagca gacgatccac atcgcctaca
     3961 tcacccccgg actgaacgta cccttctgga aatacctctc cgacggtatc aagcagaagg
     4021 ccgcccagct cagttcggag aacggggtcg acatccaggt caccgattac gactcccgca
     4081 acagtgccca gacccagctc cagaatgcgc aggacgtggt taccgccggc gtcgacgcga
     4141 tcctgatctc gccaactgac tcctcgacct gcccggcggt gctcgaggtc gctgcggagg
     4201 ccaacgtgcc ggtcatcatc agcgacatcg gcaccgactc cggcgattac gtctccttcg
     4261 tcatctcgac caacgagcag ggcgcctacg acgccggcca ggtcctgatc aaggagatgg
     4321 acgccaaggg ctggaagggt gccgacgtcc tgatgatcac catctcgcag gcccgtcaga
     4381 atggcaagaa ccggacaaag ggcgtcaccc gggcggtcga agagggtggc tccaagatcg
     4441 ttcagttcct gcagagcgag cagtacaccc gggccgaggc gcagggccag gcgctcgacc
     4501 tgatgaccgc caaccctgac gcgcacggct tcttcacgca gcacgatgag gcgacgctcg
     4561 gcacttgggc ggcgatcgag gacgccggca acacggacaa catcatcctg gtcggcttcg
     4621 acggtagccc ggagtcggtc gagttgatcc ggcaaggcaa gctccggggc gcctcgatgc
     4681 agcagccggt gctgatgggc cgcgattcga tggatgtcgc ttggcggcac ctgcaggggc
     4741 aggacgtcga gaaggagacc tcggttccga cgatcctcgt tacgccggat aacctgaagg
     4801 acaccgagaa gacgctgcag gacactgtct tcccggtcga aggcggtgcc acgccgggag
     4861 ccagcccgac gtcgtagcgc ggcgcggtag ccccgcgtta cgtgcaaatg aatggggcgg
     4921 ccgcgatcgg ccgccccaca ttctttggat atcggagaaa gctacggtag cgggtcgatt
     4981 gggctcagat tcgagccgtc caggctcgag cggtcgacgc ggaccgggac tgtgatcgtg
     5041 ccgccggttg agaccgggtc gatcggcgcg ggctcggacg actgctgcac cccaccgatc
     5101 tggctctcat cgaccgagct gccatcgagc ggatcctgtg gcgccggtcc cggatccacg
     5161 cccgagatct ggctctcgtc gacggcgatc ccgtccagcg gccccttcgg ctgaataacc
     5221 aggctggcct tgcaggtggt gtcgttcttg gtgttcgtgc aggtctgcgg agtctcacca
     5281 ccggtgcagg tggtcacgtt cttgatgaac tgatggccgt tggtatagct tccatagtag
     5341 tccctcgatt cgaacgttcc gccgctgacc atgcataggt cactctgggc ctgaacccga
     5401 tccgagactg ataccccctt ggctggtctc gcggccactc cctgggccag catcagcccc
     5461 aggatgaggg caaacgcccc ggccatcatc acgcgcgaaa agatcacggt cgctgctgtc
     5521 ttcccggtcc tcatctcgat cctcctcagt gacgctcaac cattccgttg ctgccatcct
     5581 gcatccggaa aagcgccgtc acatcggtga gatcacgtaa atgg
//
LOCUS       DATAOZ010000178         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3493448, whole genome shotgun sequence.
ACCESSION   DATAOZ010000178 DATAOZ010000000
VERSION     DATAOZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3493448"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..>1641
                     /locus_tag="VHR64_04810"
     CDS             11..>1641
                     /locus_tag="VHR64_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX3302180.1"
                     /translation="MAGSIGPTGLLPASEDPLLGNHRFGDVVELFVNQVRGLADGGSD
                     LFIIETTQDILELKAAVHAILRVREETGVRIPIQAQVTLDTAGRMLLGTDIAAALAVL
                     ESLPVDVVGLNCSTGPEHMREPARYLGEHATRPVAVIPNAGLPLNVDGRAVYPLAPDE
                     MARDLCEMVEQFGVGIVGGCCGTTPDHLAALVREIGRRPTSTRPARPLPMTASMVRAV
                     DLRQDPAPLIVGERVNTLGSRKVKRLALADDYDGMIVVAREQVESGAHALDVCVAMTE
                     RTDEREMLRALVKKLALNVEAPLVLDSTEADVLKDALEQYPGRAIVNSINMENGRLKI
                     DSVMPLAIAHGAAVVAMTIDEEGMAKTAHRKLEAARKIHDIVVGEYGLAPEALIFDPL
                     TFPLSTGDEEFVRSALETLDGIRAIESDMPGVMTILGISNVSFGLNPVARKIVNAVFL
                     YHAVEAGLDLAIVHPSHVMPFAEIPPIERELAEDLILARRDDALARLIEHFEGREEET
                     AADGADPMADMTPEEKLHYQIVHRKKEGIEEQIDLA"
BASE COUNT          294 a          489 c          565 g          293 t
ORIGIN      
        1 gccgcgatac gtggccggct ccatcggccc aacgggtttg ctccccgcgt ccgaagaccc
       61 gctcttggga aatcaccgct tcggcgacgt ggtcgagctc ttcgtcaacc aggtccgtgg
      121 gttggctgac ggtggtagtg acctgttcat tattgagaca acacaggaca tcctcgaact
      181 gaaggcggcg gtgcatgcca tcctgcgcgt gcgggaggag accggtgtcc ggatcccgat
      241 ccaggcccaa gtgacgcttg acacggccgg tcgcatgctg ctaggcacgg acatcgccgc
      301 cgccctggcg gtcctggaat cacttccggt cgatgtcgtc ggtttgaact gctcgaccgg
      361 gccggagcat atgcgcgagc cagctcggta tctcggtgag cacgcgacgc ggccggtcgc
      421 ggtcatcccc aacgccggat tgccattgaa tgtggacggt cgggcagtct atccactggc
      481 gcctgatgag atggctcgtg atctgtgcga aatggtcgag cagtttggag tcgggatcgt
      541 cggcggctgc tgcggcacga cgccggacca tctggcggcc ctggttcgcg agatcgggcg
      601 gcgtccaacc agtacgcgcc cggcgagacc actgccgatg acggcgtcga tggtgcgtgc
      661 ggtcgacttg cggcaagatc cggcgccatt gatcgtcggc gagcgggtca atacgctcgg
      721 ctcgcgcaaa gtcaagcgcc tggcgctggc ggacgactac gacggaatga tcgtcgtcgc
      781 ccgcgagcag gtcgagagcg gtgcgcacgc gctcgacgtc tgcgtggcga tgacggagcg
      841 gacggacgaa cgggagatgc tgcgcgcgct ggtgaaaaag ctggcgctga atgtcgaggc
      901 gcctctcgta ctcgactcca ccgaggcaga tgtcctcaag gacgccctgg agcagtaccc
      961 cggccgcgcc atcgtcaatt cgatcaatat ggagaatggg cggctcaaga tcgactcggt
     1021 catgccgctg gccatcgcgc acggcgccgc cgtggtggcg atgacgatcg acgaagaagg
     1081 tatggcgaag acggcgcacc gcaagctgga ggcggcgcgc aagatccacg acatcgtggt
     1141 cggcgagtat gggctagcgc ccgaggcgct gatcttcgac cctctgacgt ttccgctttc
     1201 gaccggtgat gaagagttcg ttcgctcggc gcttgagaca ttggacggca tccgcgccat
     1261 cgagtcggac atgcccgggg tgatgacgat cctgggcatt tccaatgtct cgttcgggct
     1321 caatccggtc gctcgcaaga tcgtcaatgc ggtgtttctc taccacgccg ttgaagctgg
     1381 gttggacctc gcgatcgtcc atccctcgca tgtcatgcct ttcgccgaga ttccgcccat
     1441 cgagcgcgag ctggcggagg acctgatctt ggcgcggcgg gacgatgccc tggcgaggct
     1501 gatcgagcat ttcgagggac gagaggaaga gacggctgcc gacggggcgg acccgatggc
     1561 cgacatgacg ccggaggaga agctgcacta ccagatcgtg catcgtaaga aagaggggat
     1621 cgaggagcag atcgacctgg c
//
LOCUS       DATAOZ010000179         4431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1917732, whole genome shotgun sequence.
ACCESSION   DATAOZ010000179 DATAOZ010000000
VERSION     DATAOZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917732"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VHR64_04815"
     CDS             <1..525
                     /locus_tag="VHR64_04815"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HEX3302181.1"
                     /translation="WHLDGSVHRFTGTYTEGQEAGATKLIDAAIREVPVHDMPPLCAA
                     SDLEATLTGDAGAGQRYGTVTATNVAAGSCVLGGIPRVVVTDAAGNPVISARPEPNVP
                     ITTVTLAPGKSATLGIHWSNWCGTSVSGEPQVSVTFRGGMGRIANLTGLAVPPCLSEP
                     GGASTLNVKPWQIG"
     gene            614..1012
                     /locus_tag="VHR64_04820"
     CDS             614..1012
                     /locus_tag="VHR64_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX3302182.1"
                     /translation="MLSDAVRDFLNERRFAVLATSNPNGTIQQSVMWYLLEDNTIVMN
                     TAKGRIKFRNMEHSNRISLCVEDGLKFVTLTGSVSFNDDQAVAQEDIQRIGRRYDSEE
                     EVRDAHEQNWKHQQRVTLTMPIERVVTHGF"
     gene            1028..1975
                     /gene="cofD"
                     /locus_tag="VHR64_04825"
     CDS             1028..1975
                     /gene="cofD"
                     /locus_tag="VHR64_04825"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX3302183.1"
                     /translation="MIATLAGGVGGARFIDGLVQAAGAEGVVAVVNTADDFELYGLRI
                     CPDLDTVLYTLAGLANPVLGWGIADDTRATLDGIAAYGEEPWFLLGDRDFATHILRTA
                     WLREGRTLSDVIDELQRRLGVRTPIIPMTNDTVGTTIETPGGWMDFQDYFVRRRQQDA
                     VIGVSFVGIEEAHPTATAMEAIRHADMIMLAPSNPIVSIGPILAIDDFHRAISDRSVP
                     ALAISPLIGGKALKGPADRMMETLGHESTALGVARLYEGLIDGFVIDHVDAEQAAEIE
                     ELGIAVLVTDAIMHDVADRGRLAREVVAFGESLHVKATA"
     gene            1972..2622
                     /gene="cofC"
                     /locus_tag="VHR64_04830"
     CDS             1972..2622
                     /gene="cofC"
                     /locus_tag="VHR64_04830"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX3302184.1"
                     /translation="MSSNTLAIIPVRSLSTGKSRLAPAVTPEKRRELTKRMLAITLGA
                     CRDSAVIDQILVISPDVGALAFSHAIDHEVIGIRQNQQTPGLIPALEQARRVGAVNRF
                     DALLILFADLPLVNGDDVAELVATPGQIVIAPDQNRAGTNGLLLRRGPVELSEFAFRF
                     GVGSFAAHVAEARQLGIEPAIVDLPGLGFDLDTPDDLGNLISLDSAFMSRLRETGS"
     gene            2619..3374
                     /gene="cofE"
                     /locus_tag="VHR64_04835"
     CDS             2619..3374
                     /gene="cofE"
                     /locus_tag="VHR64_04835"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX3302185.1"
                     /translation="MTAIQIIGVDHVPEVRPGDDVVRLIANGMRGSETLPQDGDVVVV
                     THKIVSKAEGQLVDLNLIEPSALAVQWAEKYDKDARQIEVVLREAVRIVRMERGILIA
                     ETRHGFICANAGVDASNVSPHTVCLLPVDPDRSAAQIRDGLCREFGVDVGVVISDSFG
                     RPWRNGIINVAIGVAGIPALADYRGQTDAAGYSLHVTVLAIADEIAAAAELIMNKLDA
                     RPVAIVRGYQFPVAAEPGTGRDLVMDPSRDLFR"
     gene            3389..4384
                     /locus_tag="VHR64_04840"
     CDS             3389..4384
                     /locus_tag="VHR64_04840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX3302186.1"
                     /translation="MLVNGIGYAASFEQFDPNDMLTWSQQAEQQGFISVIASDHFHPW
                     VPSQGQSAFVWSWMGALGATTKLRFGTGVTPPGYRYHPAVLAQAAATLEVMFPGRFYL
                     GLGAGEALNEHITGDYWPEAPVRLERLAESIEIIKQLFTGKIVKYRSQHFNVESAKLY
                     TLPSTPPPIYVATSGPIQAKRTGRTCDGLITVGAADDKLKMLMTRFEEGAKEAGKDPA
                     TMPKIIQFKVSLAKTEDAAIEQAIKEWPNGGMSFPKGDIRNPEDFEAMAKLVRPEHYK
                     NRVLTSPNLDEHIKLIQHYVDLGFNEVYVHNVGRNQAEFVETYGKHVIPNVKFPG"
BASE COUNT          811 a         1451 c         1419 g          750 t
ORIGIN      
        1 tggcatttag atggctcggt ccaccgcttt accggcacct acaccgaggg ccaggaagcc
       61 ggcgccacca aactgatcga cgccgcaatc agggaggtgc cggtccacga catgccgccg
      121 ctctgcgccg cgagcgatct cgaggcgacc ctgaccggtg atgccggtgc gggtcagcgc
      181 tatggcaccg ttaccgcgac gaacgtcgcc gccggctcct gcgtgctcgg cggtatcccg
      241 agagttgtgg tgaccgatgc ggccggcaac cccgtgatct cggcgcgacc ggaaccgaac
      301 gtcccgatca cgaccgtcac gctcgctccg ggcaagagcg ccacgctcgg catccattgg
      361 tcgaactggt gcggaacatc ggtcagcgga gagccacaag tctcggttac attccgcggc
      421 ggcatgggac gaattgcgaa cctgaccggt ctcgctgtgc cgccgtgcct gagcgaaccc
      481 ggtggtgcat cgaccctgaa cgtcaagccc tggcaaattg gttaggctgg caatcggttg
      541 acggccccgg cggcctctcc tagcattact ttccgaattg aagctttatg ataacgaagg
      601 ggattccttt tcgatgctga gtgatgccgt ccgcgacttt ctgaatgagc gccgtttcgc
      661 cgtcctggcc acctcgaatc ccaatgggac aatccagcaa tcggttatgt ggtacttgct
      721 ggaggacaac acgatcgtga tgaacaccgc caaggggcgg atcaagttcc ggaatatgga
      781 acactcgaac cggatctcgc tttgcgtcga ggacggactg aagttcgtca ccctgaccgg
      841 cagcgtcagc ttcaacgacg atcaggcagt cgcgcaggaa gacatccagc ggatcggccg
      901 gcgctacgat tccgaggaag aggtgcggga cgcgcacgag cagaactgga agcaccagca
      961 gcgcgtcacg ctgacgatgc cgatcgagcg cgtcgtcacg cacggcttct aggcgggacg
     1021 tcagcaggtg atagcgaccc tggccggagg agtaggcggc gcccggttca tcgacggcct
     1081 ggtccaggct gccggggccg aaggcgtcgt cgcggtcgtc aataccgccg atgacttcga
     1141 gctctacggg ctgcgaatct gcccggacct cgataccgtc ctctacacgc tggccggcct
     1201 ggcgaaccct gtcctcggct ggggcatcgc cgacgatacg cgcgcgacgc tcgatggcat
     1261 cgctgcctac ggcgaggaac cctggttcct gctcggcgat cgcgacttcg ccacccacat
     1321 cctgcgcacg gcctggctgc gcgagggtcg cacgctctcg gatgtcatcg atgagctcca
     1381 gcgtcgcctc ggcgtgcgga ctccgatcat cccgatgacc aatgacacgg tggggacgac
     1441 gatcgagaca cccggcggct ggatggactt ccaggactac ttcgtacgcc gtcgtcagca
     1501 ggacgcggtg atcggtgtct cgttcgtggg catcgaagag gcgcacccaa ccgcaactgc
     1561 catggaggcg attagacacg cggacatgat catgctcgcg ccatcgaatc cgatcgtcag
     1621 catcggccca atcctcgcga tcgacgattt ccaccgcgca atctccgacc ggtcggtccc
     1681 ggcgcttgct atcagcccgc tgattggcgg caaggcgctc aaggggccag cggaccggat
     1741 gatggagacg ctcgggcacg agtcgaccgc gctcggcgtg gcccggctct acgaggggct
     1801 gatcgacggc ttcgtgatcg accacgtcga cgccgagcaa gccgcggaga tcgaggagct
     1861 cggcatcgcg gtcctcgtca ctgatgcgat catgcacgat gtcgccgacc ggggccgttt
     1921 ggcccgggag gtcgtcgcat tcggcgaatc gctccacgta aaggccactg cgtgagcagc
     1981 aataccctcg caatcatccc cgtccggtcc ctgtcgaccg gcaagtcgcg cctggcgccg
     2041 gctgtgacgc cagagaagcg gcgcgaactg accaagcgca tgctggcgat cacactcggg
     2101 gcgtgtcgcg actccgcggt catcgatcag atcctggtca tctcgccgga cgtcggggcg
     2161 ctggcctttt cccacgcgat cgatcatgag gtgatcggca ttcgccagaa tcagcagacg
     2221 cccggcctga tccccgccct cgaacaggcc cggcgggtcg gcgcggtcaa ccgcttcgac
     2281 gcgctcctga tcctcttcgc cgatctgccg ctggtgaacg gagacgatgt tgccgagttg
     2341 gtcgcgacac ccggccaaat cgtgattgca cccgaccaga accgggccgg cacgaacggg
     2401 ctgctcctgc gccgtggacc ggtcgagctc tccgaatttg cgttccggtt cggcgtcggc
     2461 agctttgcgg cccatgtggc cgaagcgcgc cagctcggga tcgagccggc catcgtcgac
     2521 ttgcccgggc tcggtttcga cctcgacacg cccgatgacc ttggcaacct gatctcgctc
     2581 gactcggctt tcatgtcgcg cctgcgggaa acgggatcat gacggcgatc cagatcatcg
     2641 gcgtagacca cgtccccgaa gttcgtcccg gcgacgacgt cgttcgcctg atcgcgaatg
     2701 gcatgcgcgg gagcgagaca ttgccgcagg atggcgacgt ggtggtcgtc acgcacaaga
     2761 tcgtcagcaa ggccgagggc cagctcgtcg acctgaacct gatcgagccg tccgcgctcg
     2821 cggtccagtg ggccgagaaa tacgacaagg acgcccgaca gatcgaggtc gtgctccgcg
     2881 aagcggtccg gatcgtgcgc atggagcgcg gcatcctgat cgccgagacg cggcacggat
     2941 tcatttgcgc gaatgccggg gtcgacgcct cgaacgtcag tccgcacacc gtctgcctgc
     3001 tgccggtcga ccctgatcgc tcggcggcgc agattcgcga tggcctgtgt cgtgagttcg
     3061 gtgtcgacgt cggggttgtc atctccgact catttggccg gccgtggcgg aacgggatca
     3121 tcaacgtcgc gatcggggtc gccggtatcc cggcgctagc cgactatcgc ggccaaactg
     3181 acgcggccgg ttacagcctg cacgtgacgg tgctggcgat cgccgatgag atcgccgcgg
     3241 ccgccgagct gatcatgaac aagctggacg cgcggccggt cgcgatcgtc cgcggctacc
     3301 aattcccggt cgcggcggag ccgggcactg gacgcgacct ggtcatggac ccgtcgcgcg
     3361 atctgtttcg ataagggaag gaatggagat gctggtcaac ggtattggat acgccgcgag
     3421 cttcgagcaa ttcgatccga acgacatgct gacgtggtcg cagcaggccg agcagcaggg
     3481 gttcatcagc gtgatcgcgt cggaccactt ccacccgtgg gtgccgagcc aggggcaatc
     3541 cgccttcgtt tggtcgtgga tgggtgcgct cggcgcgacg accaagctcc gcttcggcac
     3601 cggcgtcaca ccgcctggat accgatatca cccggccgtt cttgcccagg cagccgcgac
     3661 actcgaggtc atgttccccg gccgctttta ccttggtctc ggcgcgggtg aggcgctcaa
     3721 tgagcacatt accggcgact actggccgga ggcgccggtc cgtctcgagc ggctggccga
     3781 gtcgatcgag atcatcaagc agctcttcac cggcaagatc gtcaagtacc gctcacagca
     3841 cttcaacgtc gagagcgcga agctctacac cctgcccagc accccaccgc cgatctacgt
     3901 cgcgacctcg ggaccgatcc aggccaagcg gacgggcagg acgtgcgacg ggctgatcac
     3961 cgtcggtgcg gcggacgaca agttgaagat gctgatgacc cgcttcgaag aaggcgcgaa
     4021 ggaggccggc aaggaccccg cgaccatgcc gaagatcatc cagttcaagg tctcgctcgc
     4081 caaaaccgag gacgcggcca ttgagcaggc gatcaaggag tggcccaacg gcggcatgtc
     4141 cttcccgaag ggcgatatcc gcaaccccga agacttcgaa gcgatggcca agctggttcg
     4201 gcccgagcac tacaagaacc gggtcctgac ctcgccgaac cttgacgagc acatcaagct
     4261 gatccagcac tacgtcgatc tcggcttcaa cgaggtctac gtccacaacg tcggccgcaa
     4321 ccaggccgaa ttcgtcgaga cctacggcaa gcacgtcatc ccgaacgtca agttcccggg
     4381 gtaggcaccc agcggtagtg gcggagctcc gtacccgcct ggagctgccc g
//
LOCUS       DATAOZ010000180         7542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8232622, whole genome shotgun sequence.
ACCESSION   DATAOZ010000180 DATAOZ010000000
VERSION     DATAOZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8232622"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VHR64_04845"
     CDS             complement(<1..236)
                     /locus_tag="VHR64_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302187.1"
                     /translation="MSWERAVLIGLLVFLAGCHYVLAWYSLQDLTRRTAVRGGNRTFW
                     GLFILIVPLAGALVYGMYGPQGFLSRDRPPRATIA"
     gene            complement(408..1748)
                     /locus_tag="VHR64_04850"
     CDS             complement(408..1748)
                     /locus_tag="VHR64_04850"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEX3302188.1"
                     /translation="MIQFRLRQIPSTARWLTLVLLLILLSGASVSLAQDNNTVSPIGE
                     AVTVGPWQMTVERVLTGTDAAAAAAGASASNPDPAEGLQYVAAQINVQNSSSQPFMIG
                     PEDFAVVGSSGILRRSAGVAAPSPELDGIVRPGESADGWILLSTEADAENLVLIYDST
                     TITGSWADHIFAISDGATFAPSTTRAAEIDKDGESPDKPVGVGRVIATSEWRIKIVDV
                     VQGAAVIDIAPEETKRLGQSYNSGDGTYASCLDTWIAIQVEATNNGDDGLTRFLSPTA
                     FQLADTDGSALNDIRTLSPPAPDLSGEYAAGASRTGWISFELPSNCGPGGANISYDAD
                     LLRFQPFATSEDVRYLTWTGGDGPEPADTPTPKPFDSSTALAEGSTAVTTDDGVRMRS
                     DPSVDGEIVDELDKGTEVEITGPPEEGDGFTWYPVRNPENDDEGWIVQDFLAAP"
     gene            1834..3768
                     /locus_tag="VHR64_04855"
     CDS             1834..3768
                     /locus_tag="VHR64_04855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX3302189.1"
                     /translation="MTKTTAPYGSWKSPITSDLIVSGTIGLSDAVVSGDNIYWIESRP
                     SERGRRSIQRWSADGTITELTPPAFNVRTRVHEYGGASYEVVGETIYFQNYADQLLYR
                     HDPGADPRPITTSGIFYGDFRYDAGRDRLVSIQEDHGVSDIEAINTLVSLNPNGPNAD
                     GGVNLVSGADFLTNPRLSPDGRKMAWLEWSHPNMPWDAAELWVGDVDAEGAIHNGRHI
                     AGGNGSSVFQPEWTPGGDLIFVDDRTGWWNFYSYRDGAVTHLLDMEAEFGRPLWVVGM
                     STYVVISDERLICAYTQNGSWYIGDVDLTTGKLTTVPTPFTDVSSIAAEGNIVAFVGG
                     SATQPTGLYRHDLTTGETTQIKSASTIAIDPDYLIEPTTVTFPTSDDQVAHAFLYLPR
                     NKDFAGPDGELPPLIVSSHGGPTSAALTSMELRFQFWTSRGFAVLDVNYRGSTGYGRP
                     YRDALRDTWGIYDVDDCVYGAQYLVEQGIVDGDRLAVRGGSASGYLTLAALTFRDTFD
                     AGCALYGISDLEIMALETHKFESRYLDGLIAPYPEGIEVYKERSPLYHIDQINTPLIL
                     FHGLEDKVVPPNQSELIYEALKKKGVPVAIVLYEGEQHGFRQAANIKRTLDGELYFYS
                     RIFGFEPADQIDPIPIANLD"
     gene            3765..4694
                     /locus_tag="VHR64_04860"
     CDS             3765..4694
                     /locus_tag="VHR64_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3302190.1"
                     /translation="MSAGTSGKIRTIPAVAGLKRLEGETVAYLALVATAVIWGTTAIG
                     IKVALETYPPFILSVGRWLISLAIMLPMMRRFGVRPILDRRTFVLGLFGILGFNAFFT
                     LGLERTTAANGSLINGALPVVVAFLSFLLLSERLAPVRILGIVISMVGVAVTVLGATL
                     DASVLGNVLIFGAVLSWAIYTIYNRERMKGENTMGIIAGSALFGVTMMVPLAAIEWAQ
                     ETPEAPSLKLVAIILYLSLGPAMAANYMWVFALTRVPASQAAVFSNLTPIVGIVLAGL
                     ILDEPITKYHVVGSILVIAGVLLTTWRRQPPKT"
     gene            complement(4978..6462)
                     /locus_tag="VHR64_04865"
     CDS             complement(4978..6462)
                     /locus_tag="VHR64_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3302191.1"
                     /translation="MRGQILRLLIALTLGIGLIGPGLQFARAQNSGGIAIGDTVSIGE
                     DGVNMRKTPGTNGEIITTLNTGTSLEVLDGPTTASGYTWWLGVVLNEDSVDQGISGWV
                     IEDALTVEDVQSTPTPDTGTTTTPTPEPSTSPTATATPGTGDTTFENAKWVQVVDGPV
                     NLRKNPGLQGSVIRALGTDETATRVDPSELTERDGYTWINIKTQAGETGWAATDFLDP
                     LSTDPCPSGTCEPAQHQDLLDANAVVIVDGPLNVRSSASVDGEILDTVATGAVLNTAT
                     NGQITTAGDYDWLKVDYQGQEAWVAVDFVSVSDETCDVSPCFPEGTSTDDPFVGALGV
                     EVFDGPLNVRDVAGLDGTIIDVLDTGDEAPVDTRAVLTDADGYTWIKIVTAEGGGWVA
                     TDFVHPLDQVPCGDVVCYPSELSSFIGASGAFVTDGPLNLRSEMGTNSSILMTLDNGD
                     YLTIESVAGTEQAYEADGYIWIQVTPAGTNLTGWVAIDFVTPAG"
     gene            complement(6658..>7542)
                     /locus_tag="VHR64_04870"
     CDS             complement(6658..>7542)
                     /locus_tag="VHR64_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3302192.1"
                     /translation="KDGPQAKNGYDWYKVILLGDSDETPINGWVAADFIALEDNGQDF
                     AKAKWVVVSDGPVNVRTGAGTSNSIVTTMADEETASVKGSAGLKTANGYTWINVLLNS
                     GSSGWVATDFLSVLTSDPGDGGNNGGDYATAEGVEIVDGPVNVRSKPSLSGTITSVQD
                     TGLKFFIVADSDLVDADGYSWVNIMNFGGVRGWVATDFTAPVADMPCGDGACYPEELN
                     PFFEASAATVTDGPVNLRASAGTSSQILLTLQNGDYLWLSKPITDHVEEADGYTWIQV
                     SVAGKTGWIAIDFISPAD"
BASE COUNT         1319 a         2485 c         2371 g         1367 t
ORIGIN      
        1 gcgatcgtgg cgcgcggcgg ccgatcgcgc gagaggaagc cctgcggacc gtacatcccg
       61 tagacgagcg ccccggccag gggcacgatc aatatgaaga gaccccagaa ggtgcgattg
      121 ccgccccgga ccgccgttcg ccgcgtaagg tcctgcagac tgtaccaggc cagcacgtaa
      181 tggcaaccgg ccaggaagac cagcaagccg atcaagacgg cgcgttccca gctcacgtcc
      241 tgcactccgc caaatgtcgt agcgccgggc cgccgtgccc cgcttcgttc atccccacac
      301 acgatctata gagaccgaat cgagaacggg gtttgcctcc aatatagatg ccatcctcga
      361 gcgcgggcgg ggcatggtaa ccccgcgcta cgtttgcagc ggcacgtcta gggcgcggca
      421 aggaagtcct gcacaatcca gccttcatcg tcattctcgg ggttgcggac cggataccat
      481 gtaaagccat caccctcttc gggcgggccg gtgatctcga cctcagtgcc cttgtccagc
      541 tcatcgacaa tctcgccatc gaccgatgga tccgaacgca tccggacgcc gtcatcggtc
      601 gtaacggcgg tcgagccctc agccaatgcc gtgctggaat cgaatggctt cggtgtcggc
      661 gtatcggctg gctctgggcc gtccccacct gtccaggtca ggtagcggac atcttcggac
      721 gtggcgaacg gctggaagcg gagcaaatcg gcgtcatagg agatgttcgc tccgcccggg
      781 ccgcagttgc tcggcagctc gaacgagatc cagccggtgc gggaagcgcc ggccgcatac
      841 tcgccgctca gatccggcgc cggcggcgag agtgtgcgga tgtcgttcag ggcggagccg
      901 tccgtgtcag caagctggaa tgcggtcggc gagaggaagc gggtcagacc gtcgtcgcca
      961 ttgttggtcg cctcaacctg gatcgcaatc caggtgtcga ggcaggaggc ataggtgcca
     1021 tcgccggagt tgtagctctg gccgagccgc ttggtctcct caggggcaat gtcgatcacg
     1081 gccgcaccct ggaccacatc gacgatcttg atccgccact cgctggtcgc gatcacgcgg
     1141 ccgacgccga ccggcttgtc cgggctctcg ccgtccttgt cgatctcggc cgcgcgcgtg
     1201 gtcgatggcg cgaaggtcgc gccgtcgctg atcgcgaaga tgtggtcggc ccacgacccg
     1261 gtgatcgtcg tgctgtcgta gatcagaacc agattctcgg catcagcctc ggtgctgagc
     1321 agaatccagc catcggccga ctcgcccggc cggacgatgc cgtccagctc gggtgatggc
     1381 gccgcgacac cggccgagcg ccgcaggatg ccgctcgagc cgacgaccgc gaagtcctcc
     1441 gggccgatca tgaagggctg gctggagctg ttctggacgt tgatctgcgc cgcgacgtac
     1501 tggagccctt cggccggatc ggggttggag gcacttgcac cggcagccgc tgcggccgca
     1561 tctgtgccgg tcaacacccg ctcgaccgtc atctgccacg gcccgaccgt gaccgcctcc
     1621 ccgatcggac tgacagtgtt gttgtcctgc gccaggctga ccgatgcgcc actgagcaga
     1681 atgagcagca agacgagggt caaccaacgg gccgtcgagg gaatctgtcg caagcggaac
     1741 tggatcatgc aatcgctttc ctggggtgga cattcgcccg gttccaacgc attgtacggg
     1801 ctgcgtggca cgcaggtcac agggaggcat ttggtgacga aaacgacagc cccgtacggc
     1861 tcctggaaat ccccgatcac atcggacctg atcgtttccg gcacgatcgg cttgagtgat
     1921 gctgttgtct cgggcgacaa catctattgg atcgaatcgc ggccgtccga gcgtggtcgt
     1981 cgctcgatcc agcgctggtc cgcggatggc acgatcaccg aactgacgcc gcctgccttc
     2041 aatgtccgca cccgtgtcca cgagtacggc ggggcatcgt acgaggtggt cggcgagacg
     2101 atctatttcc agaactacgc cgaccagctc ctctaccggc acgaccccgg cgccgatccc
     2161 cgcccaatca cgaccagcgg catcttctac ggcgacttcc ggtatgacgc cggccgtgat
     2221 cgcctggtca gcatccagga ggaccacggc gtctccgaca tcgaggcaat caatacgctc
     2281 gtctcgctca acccgaacgg accaaatgcc gacggcggcg tgaacctggt cagcggcgcc
     2341 gacttcctga ccaatccgcg actcagcccg gacggccgca agatggcctg gctcgagtgg
     2401 agccatccga atatgccttg ggacgcggcc gagctctggg tcggtgacgt cgatgcggag
     2461 ggtgcgatcc acaatgggcg ccatatcgcc ggcggcaacg gctcatctgt ctttcagccc
     2521 gaatggacgc cgggcggaga tctgatcttc gtcgacgacc ggaccggctg gtggaacttc
     2581 tacagctacc gggacggcgc cgtcacgcac ctgctcgata tggaggccga gttcgggcgg
     2641 ccgctctggg tcgtcggcat gtcgacctac gtggtcatct ccgacgagcg gctgatctgc
     2701 gcctacacgc agaacggctc ctggtacatt ggagacgtcg acctcactac cggcaagctc
     2761 acgaccgtac cgacgccgtt caccgacgtc agctcaatcg ccgccgaggg caacatcgtc
     2821 gccttcgtgg gcggctccgc gacgcagccc accgggctct accggcacga cctgaccacc
     2881 ggcgagacga cgcagatcaa gtcggcgagc acgatcgcaa tcgaccccga ctacctgatc
     2941 gagccgacga ccgtcacatt cccgaccagc gacgaccagg tcgcccatgc cttcctctac
     3001 ctgccacgta acaaggactt cgccgggccg gatggcgagc tcccaccgct gatcgtcagc
     3061 agccacggcg ggccgaccag cgccgcactg acctccatgg agctgcgctt ccagttctgg
     3121 accagccggg gatttgccgt gctcgatgtc aactaccgcg gcagcaccgg ttacggccgg
     3181 ccataccgtg atgccctgcg cgacacctgg ggcatctacg acgtcgatga ctgcgtctac
     3241 ggggcgcagt acctggtcga acagggcatc gtcgacgggg acaggctcgc cgtccgtggc
     3301 ggcagcgcga gcggatatct gacgctggcg gcgctgacct tccgcgacac gttcgacgcc
     3361 ggctgcgccc tctacggcat cagcgacctc gagatcatgg ccctcgagac gcacaagttc
     3421 gagtcgcgct accttgacgg tctgatcgcg ccctatcccg aggggatcga ggtctacaag
     3481 gagcgctcac cgctctacca catcgaccag atcaacaccc cactgatcct cttccacggc
     3541 ctggaagaca aggtcgtacc gccaaatcaa tcggagctga tctacgaggc actgaagaag
     3601 aagggtgtgc ccgtcgcgat cgtcctctac gagggcgagc agcacggctt ccggcaggcc
     3661 gccaacatca agcgcacgct cgacggcgag ctctatttct acagccgcat cttcggcttc
     3721 gagccggccg atcagatcga cccgatcccg atcgcgaacc tcgattgagc gcgggcacgt
     3781 ccggcaagat ccgcactata ccggccgtgg caggtctgaa gcggctcgag ggggagacgg
     3841 tcgcctacct ggccctggtc gccactgccg tcatttgggg cacgaccgcg atcgggatca
     3901 aggttgcgct cgaaacctac ccgccgttca tcctctcggt cgggcgttgg ctaatctcac
     3961 tcgcgatcat gctgccgatg atgcgacggt tcggcgttcg cccgatcctg gatcggcgca
     4021 cgttcgtgct cggcttgttc ggcatcctcg gcttcaacgc cttcttcacg ctcggcctgg
     4081 agcgaacgac ggccgcaaat ggctcgctga tcaacggcgc cctgccggta gtcgtggcgt
     4141 tcctgtcgtt cctgctgctc agcgagcggc tggctccggt ccggattctc gggatcgtga
     4201 tctcaatggt gggcgtggcg gtcaccgtcc tcggcgccac gctcgatgcc tcggtccttg
     4261 gcaatgtgct gatctttggc gcggttctct cctgggccat atacacgatc tataaccggg
     4321 agcggatgaa gggcgagaac acgatgggca tcatcgcggg atccgctctc ttcggcgtga
     4381 cgatgatggt gccgctggcg gcgatcgaat gggcgcagga gacaccggaa gcgccgagcc
     4441 tgaagctggt cgcaatcatt ctctacctca gcctcggacc ggcgatggcc gccaattaca
     4501 tgtgggtatt tgcgctgacc cgcgtcccgg ccagccaggc ggccgtcttt tcgaatctga
     4561 cgccgatcgt cggcatcgtg ctggccggcc tgatcctgga cgagccgatc acgaagtatc
     4621 acgtcgtcgg ctcaatcctc gtcatcgccg gcgtcctcct caccacctgg cgccgccaac
     4681 cacccaagac gtaataccgg ttcgccccaa gaatcgtagc gcggggttac cacgccccgc
     4741 cttgttgatc cccacgtttg ttggattagg ccgagtagtc cgcaggattg gttccccgaa
     4801 cattccgacc gtcatcgggt ccgggagggg cgtggtaacc ccgcgccagg tccaggatgg
     4861 cgagcatgca agccggccca gtcagcagca tctaggcgag tgccgtgcga gggatcgatc
     4921 acatcaaagc gccggccagt ttggccggcg ctttgatgat gtcgagcgat aactaatcta
     4981 tccggctggg gtcacgaagt cgatggcgac ccagccggtc aggttcgttc cggccggggt
     5041 gacctggatc cagatgtagc cgtcggcctc gtacgcctgc tctgtgccgg cgacgctctc
     5101 gatcgtcagg tagtcgccat tgtcgagcgt catcaggatc gacgagttag tgcccatctc
     5161 cgagcgcagg ttgagcggcc cgtcggtcac gaacgcgccg ctggccccga tgaacgagct
     5221 cagctcggac gggtagcaga ccacatcgcc gcacggcacc tgatcgagcg ggtggacgaa
     5281 gtcagtcgcg acccatccgc cgccctcggc cgtcacaatc ttgatccacg tgtagccgtc
     5341 ggcatcggtc agcaccgcgc gggtgtcgac cggcgcctcg tcgcccgtgt cgaggacgtc
     5401 gatgatcgtg ccatcgagac cggcaacgtc gcggacattg agaggtccgt cgaagacctc
     5461 cacacccagc gcgccgacga acggatcatc ggtgctggtg ccctccggga agcatgggct
     5521 gacatcgcag gtctcgtccg agacggagac gaaatcgacc gcgacccatg cctcctggcc
     5581 ctggtagtcg accttcagcc agtcgtaatc gccggctgtc gtgatttggc cgttggtcgc
     5641 ggtgttgaga accgcgccgg tcgcgaccgt gtcgaggatc tcaccgtcga cagaggcact
     5701 cgatcggacg ttgagcggcc catcgacgat gaccaccgca ttggcatcga gcagatcctg
     5761 gtgctgggcc ggctcgcagg tgccactcgg gcagggatcg gtactgagcg ggtcgaggaa
     5821 gtcggtcgcg gcccagccgg tctcgccggc ctgggtcttg atgttgatcc aggtatagcc
     5881 atcgcgctcg gtcaattcgg agggatcaac gcgcgtcgcg gtctcgtccg tgccaagcgc
     5941 gcggattacg gagccctgca ggcccgggtt cttgcgcagg ttgaccggcc cgtcgaccac
     6001 ctggacccac ttcgcattct caaatgtcgt gtcgccagta cccggagtcg cggtagcggt
     6061 cgggctggtg ctcggctctg gggtcggcgt tgtggtggtg ccagtgtccg gtgtcggagt
     6121 cgactggacg tcctcgactg tcagtgcgtc ctcgatcacc cagccgctga tgccctgatc
     6181 gaccgagtcc tcattgagga cgacgcccaa ccaccaggtg tagccgctgg cggtcgtcgg
     6241 accatccagg acctcgagcg aggttccggt gttgagtgtc gtgatgatct cgccgttcgt
     6301 gccgggggtc ttgcgcatgt tgacgccgtc ctccccgatc gagacggtgt cgccgatagc
     6361 gataccaccg ctgttctgtg ctcgcgcgaa ctgaagtccc ggcccgatca ggcctatccc
     6421 gagcgtcagc gcgatgagca atctgaggat ttgcccgcgc atgcgttcac caacctttcc
     6481 ccgctgagcc ggtactgatc gctcagcgct ccctgcccca tcggacgcat gacgggacag
     6541 cctagtttcg ccaggggaag cggtcagtgt cgcgggctac cgacagggcg gtaacgaagc
     6601 agccctgccg ggagcccgcg gtgcagcggt ggaataccgc gatgcccggt cccggcgtta
     6661 gtcggccggc gagatgaagt cgatcgcaat ccatccggtc ttgccggcca cggagacctg
     6721 gatccacgtg tacccgtccg cctcctcgac gtgatcggta atgggcttcg acagccagag
     6781 gtaatcgcca ttctgcaggg tcaacaggat ctgcgaagag gttccggcgc ttgcccggag
     6841 attcacggga ccatcggtaa ctgtcgcagc cgaggcctcg aagaatgggt tcaactcctc
     6901 gggatagcag gcaccgtctc cacacggcat gtcggccact ggcgcggtga agtccgtcgc
     6961 gacccagccg cgtacgccac cgaagttcat gatattgacc caggaatagc cgtcggcatc
     7021 gaccaggtcg gaatcggcga cgatgaagaa cttgagtccc gtatcctgga cggaagtaat
     7081 cgtgccgctc agtgagggct tgctgcggac attgaccggt ccgtcgacga tctccacacc
     7141 ttcggccgtg gcgtaatcac caccgttgtt gccgccatcg cccggatcgg acgtcaggac
     7201 cgacaggaaa tccgtcgcga cccaaccgct cgagccgctg ttcaggagca cattgatcca
     7261 cgtgtatcca ttggcggtct tgaggccggc gctgcccttg acgctcgccg tctcttcgtc
     7321 cgccatggtc gtcacgatcg aattgctggt accggcgccg gtccgcacat tgaccggacc
     7381 gtcggagacg accacccact tcgcttttgc gaagtcctgg ccgttgtctt cgagggcgat
     7441 gaaatcggcg gcgacccagc cgttgatcgg cgtttcgtcg ctatcgccga ggagaatgac
     7501 cttgtaccag tcgtagccat tcttcgcctg cggaccatcc tt
//
LOCUS       DATAOZ010000181         4060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7967517, whole genome shotgun sequence.
ACCESSION   DATAOZ010000181 DATAOZ010000000
VERSION     DATAOZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7967517"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1810
                     /locus_tag="VHR64_04875"
     CDS             161..1810
                     /locus_tag="VHR64_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha/beta"
                     /protein_id="HEX3302193.1"
                     /translation="MPNTATLTHCPKCRAPLLGRALYERFRVCDECEHHFPIEVDLRI
                     ASLVDSGSFVELDRTLASTDPLIFADAEPYRDKLRQAQTRYGRGDAATYGKATLYGNP
                     ISLLVLDFSFLGGSMGVVVGEKLARSAELARREKRALVTVVGSGGARMQEGLLSLLQM
                     AKTASQVQELKDAGLPFISVLTNPTTGGVFASFASLGDVVYAEPKSLIGFAGPRVAEQ
                     VLGRPLPPGSHSAEFLLEHGFVDSVVPRHHLRSAIALTLNTIGSNVARPKRTRLTSGP
                     LEPLEPWEAVQTARDPDRPTALHYVSECLDYWIELHGDRTGTDDRSVIAGLGQLEGQA
                     VAVIGFERGSEHDPVDRRGGRPMPGGYRKAQRLMRLASRFRIPLIAFVDTPGAYPGIE
                     SEEEGLAFEIATTMAAMIRVDAPTISVIVGEGGSGGALAFAVADRVLMQERAIYSVIA
                     PEGAAAILYRDASRAPELADQLKITAQEVRKLGISDGTIPEPKGGAKADPVTAARRVK
                     TAILDHLAEVNRMDRDKRKSTRYHRYRTIGTNYLTDLKPAR"
     gene            1943..2365
                     /locus_tag="VHR64_04880"
     CDS             1943..2365
                     /locus_tag="VHR64_04880"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX3302194.1"
                     /translation="MSTPDSKSIVRRFIEQAWIGKDVDAIQTYYAPDFSYHNPAMEGM
                     PPGLDGVRILLAGFHAAFPDADYRIDALVGDGDMVAVLYSWRGTNRGSLGLIPATDRK
                     ATATGAIFCRVAEGRIVEQWDVDDRLGVMQQLGLVPAA"
     gene            complement(2387..3097)
                     /locus_tag="VHR64_04885"
     CDS             complement(2387..3097)
                     /locus_tag="VHR64_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302195.1"
                     /translation="MALALPVSARAKEIVDMVPNPVRDPSLYRAYVPAACKAGQFFHY
                     TCEFDASWAVLKTFGIDVGLEDQFAAMEIDTRVEPYYEETPDGVLIHGGDIGRAWSGD
                     YYENFLARCTGPAIRGVFESFGLETHRIRSRRGIQQSLDAGRLVWIKATVDFHDWVPA
                     TWVTPEGLTYPVVLGNDHAVVVMGYDDEVAVIRDVLGPTNTNWNRAFEYEVDWQTFMR
                     CWAAQGKDGIAVGLPDKA"
     gene            complement(3280..3954)
                     /locus_tag="VHR64_04890"
     CDS             complement(3280..3954)
                     /locus_tag="VHR64_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302196.1"
                     /translation="MSRKTLLGLAYVLITVMLSLPFAAAAQVATPAPTPPPGAIIPDP
                     SECTVEPRPVRALEEIAATPQAVPPDADQRFSKPEQGPREWTMPAGTPADAATVDGVT
                     ATLHEALACLNANDPLRFMAPFSDEMVQLFFAMQPMPPEALPQLAASAVASPPEMWLG
                     YLQVHDVRVLPDGRVAALADDYDPTEPPYGVATDFAIFVKVGDQWLIDSLIENVVIVG
                     EATPTA"
BASE COUNT          743 a         1373 c         1224 g          720 t
ORIGIN      
        1 ctcaaaatct gggcccatga aaaaccttcg caatcttgcg cacccccagc gcacacctgc
       61 gacgtggggg acacccggat tgtacacccc gctcgcgccc gaactaccat tacagagggg
      121 gctttcgtcc cattctgaca gcacgatgga gtgaccacgc gtgccaaaca ccgccacatt
      181 gacgcactgc ccaaaatgcc gggcgccctt gctcgggcgc gcgctctacg aacgattccg
      241 cgtttgcgac gaatgcgagc accatttccc gatcgaagtt gacctccgca tcgcgtcgct
      301 ggtcgatagc ggcagcttcg tcgagctcga tcgaacgctg gcctcgaccg accccctcat
      361 ctttgccgac gccgagccgt atcgcgacaa gttgcgccag gcacaaacgc ggtacggtcg
      421 cggcgacgcg gccacctacg gcaaagcaac gctctacggg aatccgatct cgctcctggt
      481 cctcgacttc agcttcctcg gcggcagcat gggggtcgtc gtcggcgaga agctggcccg
      541 gtcagccgag ctcgcgcgtc gagagaagcg agcgctcgta accgtcgtcg gctcgggcgg
      601 cgcgcggatg caggagggcc tgctctctct gctccagatg gccaagaccg cctcgcaggt
      661 acaggagctc aaggacgccg gtcttccgtt catatcggtc ctgacaaacc cgacaaccgg
      721 cggcgtcttc gcgtcgttcg cgagcctcgg cgatgtcgtc tacgccgagc cgaagtcgct
      781 gatcggcttc gccggaccgc gggtggccga gcaggtgctc ggccgtccac ttccacccgg
      841 aagccactcg gccgaattcc tgctcgagca tggcttcgtc gactccgtcg tgcctcgtca
      901 ccacctccgc tcggccatcg ccctgaccct gaacacaatc ggctcgaatg tcgcccggcc
      961 caagcgaacc cggctcacat ccggaccgct cgagccgctc gagccgtggg aggcggtcca
     1021 gaccgcccgc gacccggatc ggccaacagc gctgcactac gtcagcgaat gcctcgacta
     1081 ctggatcgaa ctccatggcg accgcaccgg gaccgatgat cggtcggtga tcgccggact
     1141 cggtcaactc gagggccagg cggtcgccgt aatcggtttt gagcgtggtt ccgagcacga
     1201 cccggtcgac cggcgcggtg gcagaccaat gcccggcggc tatcgcaagg cccagcgttt
     1261 gatgcggctc gcctcgcgct tccgcatccc gctgatcgcg ttcgtcgaca cgccgggcgc
     1321 ctatcccggc atcgaatccg aagaggaggg cctcgccttc gagatcgcga cgacgatggc
     1381 agccatgatt cgggtcgacg cgccgacgat ctctgtgatt gtcggagaag gcggcagcgg
     1441 gggggcgctt gcctttgccg tggccgatcg ggtcctgatg caggagcgag cgatctactc
     1501 ggtcatcgcg ccggaaggag ccgccgcgat cctctatcgc gatgccagcc gcgcgccgga
     1561 gctggccgac cagctcaaga tcactgccca ggaggtccgc aagctcggca tctccgacgg
     1621 tacgatcccc gagccgaaag gcggcgccaa ggccgatccg gtcacagccg cccgcagggt
     1681 caaaaccgcc atcctcgatc accttgccga ggtgaaccgg atggaccgcg acaagcggaa
     1741 atccacccgg taccaccgct accgcaccat aggcaccaac tacctgaccg atttaaagcc
     1801 cgctcgatga tctgaacacc gggtaatttc gggacaaaac tgcaacaccg agtgaaccaa
     1861 ttctgatgct ccttggatcc ggccgttcct accttggcaa tggtcgtttg tgaccactgc
     1921 cattcgccga ggttcagttg ccatgtccac gcccgatagc aaatccatcg tccgccggtt
     1981 catcgagcag gcctggattg gcaaggatgt cgatgccatc cagacctact atgccccgga
     2041 tttctcctat cacaatccgg ccatggaggg gatgccgcca ggactcgacg gcgtccggat
     2101 ccttctggcc ggtttccacg ccgcgttccc ggatgccgac tatcgcatcg acgctctggt
     2161 cggcgatggc gacatggtcg ccgtcctcta ttcatggagg gggacaaatc gcggatcact
     2221 cgggctaatc ccggcaactg atcgaaaggc gactgccacc ggggcgatct tctgtcgcgt
     2281 ggccgagggc cgcattgtcg agcagtggga cgtcgatgac cgactcggcg tcatgcagca
     2341 gctcggcctc gtgcccgccg cctgatcact ctgcttcgag acctggttat gccttgtcag
     2401 gcaacccgac ggcgattccg tccttgcctt gggctgccca gcaacgcatg aaggtctgcc
     2461 agtcgacttc gtattcgaac gcacggttcc agttggtgtt ggttgggccc agcacgtcgc
     2521 ggatgacggc aacctcgtcg tcgtatccca tcaccacaac tgcgtggtcg ttgccaagta
     2581 cgactggata ggtcagccct tccggcgtca cccaggttgc cggcacccag tcgtgaaaat
     2641 cgaccgtggc cttgatccag accaaccgcc cggcgtcgag cgactgctgg attccgcgcc
     2701 ggcttcggat tcggtgcgtt tccagcccga atgactcgaa cacgccccgg atcgccggac
     2761 ccgtgcagcg cgccaggaag ttctcgtagt aatcgcccga ccacgcccgg ccaatgtcgc
     2821 cgccgtgaat cagcacgccg tccggcgtct cctcgtagta gggctcaacc cgcgtgtcga
     2881 tctccattgc cgcgaactga tcctcgaggc cgacatcgat gccgaaggtc ttgagcactg
     2941 cccacgacgc gtcgaactca caggtgtaat ggaagaactg cccggccttg cacgccgccg
     3001 ggacataggc gcggtagagc gacggatccc gaaccgggtt cggaaccata tcgacgattt
     3061 ctttcgcgcg agcgctgaca ggcaaggcga gagccacgcc ggctccagcc gcaacctgca
     3121 cgagatctcg acgcgtcaga cgcgactttc gaggacgtgg cggacgcgta tcgtgcctat
     3181 ttgatttccc tgaacgtcga cacattctaa acggtgtaca ccgttcaggt cggattgttc
     3241 aagcgggtca cgccggcgac aaaccagact cgcacgtact caggcagtcg gtgtcgcctc
     3301 gccgacaatc accacgttct cgatcagcga gtcgatcagc cactgatccc cgaccttgac
     3361 gaagatcgcg aagtcagtcg ccacaccgta cggaggctcg gtcggatcat agtcgtcggc
     3421 aagtgcagcg actcgcccat caggaagaac ccgcacatcg tgaacctgca gatacccgag
     3481 ccacatctcc ggtggcgagg caacggccga tgccgccagc tgcggaagcg cttcgggcgg
     3541 catcggctgc atcgcaaaga agagctggac catctcatcg ctgaatggcg ccatgaagcg
     3601 aagcgggtcg ttggcgttga ggcacgcaag cgcttcatgc agcgtcgcgg tcaccccgtc
     3661 gaccgtcgct gcgtcggcgg gtgttcccgc cggcatggtc cattccctcg gcccctgttc
     3721 aggtttcgag aaccgctggt ccgcgtctgg cggtaccgct tgcggtgtcg ccgcgatctc
     3781 ttccagtgcg cgaacgggtc ggggttcgac agtgcattcg ctcgggtccg ggataatcgc
     3841 cccgggaggt ggtgttgggg cgggcgtcgc cacttgcgca gcggctgcga acggcaacga
     3901 gagcatcacg gtgatcagga catacgccaa gccgagcagg gtcttccggg acatggcgtc
     3961 cctcctttcg tgccggtcag aacgtgattc cttgtcgcgt gccaccgccc atcctacacg
     4021 tgcaaggcaa tgcgcctcga gccaggcgcc gattagcagg
//
LOCUS       DATAOZ010000182         4859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2180415, whole genome shotgun sequence.
ACCESSION   DATAOZ010000182 DATAOZ010000000
VERSION     DATAOZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180415"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VHR64_04895"
     CDS             <1..835
                     /locus_tag="VHR64_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX3302197.1"
                     /translation="YRAELTFPQAGSWKWMITPEPFAGTSFASIQVQDAVGSTASDAS
                     APHPASIRAGSCQALGETSFPLSDVLPNGVVLDGKTDQTTGVVGVDAGVPVAISSTTV
                     DVTIAELLAEPHAISVVKQADDAGTEVACGNISGRIWNDELIVGLQQRNNSSDIGIAV
                     LREAAGKTTVSLYMMVVATEQAAATGPEVTVEITGSSGDWHFEPARVEIAPGTTVVWI
                     NRTETSHTVTGTDLAFEDSSPIAPGGTFSEVFTDPGEFRYFCSPHPFMTGVVVVTEPG
                     S"
     gene            complement(830..1204)
                     /locus_tag="VHR64_04900"
     CDS             complement(830..1204)
                     /locus_tag="VHR64_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302198.1"
                     /translation="MANHPLDAMPSGSYSPSEPRSRRTQAAQVSPYGGVGGVFLLWVA
                     FGALVLFWPDTLQRMWDQYLDWNLVVQIIVGVLLLPWAVATWVWQSDWSTLAQTLVIG
                     FLVIGTLISFAPRNPKPDAGAS"
     gene            complement(1232..1582)
                     /locus_tag="VHR64_04905"
     CDS             complement(1232..1582)
                     /locus_tag="VHR64_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302199.1"
                     /translation="MRTMMISHPNSQLDRSHWFSIDFDREPVRVIATSLVVVQVALFL
                     IGRAFDLIVGSAVAYGFFATMLLFATLVFADALSVRMPLRRAAFWAAAVTIGMIFGAI
                     PYALRRQHLTRATH"
     gene            complement(2330..2893)
                     /locus_tag="VHR64_04910"
     CDS             complement(2330..2893)
                     /locus_tag="VHR64_04910"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX3302200.1"
                     /translation="MAERLSEILRQEGEPPTPLERRALEAALIRLGEPRSPEERFAAE
                     RMTLGQRAADVVTTSVGSWTFILVQSGILAIWLIVNSLAWIRHWDPYPFILLNLVLSF
                     QAAFTAPIIMMSQNRQAARDRLEAVEDYAVDRQAEEEIEAIQNRLDELGGRQWDTLVA
                     LQREQIDLLRRIQEISTEIHRASAPEP"
     gene            3096..4739
                     /locus_tag="VHR64_04915"
     CDS             3096..4739
                     /locus_tag="VHR64_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3302201.1"
                     /translation="MSDPIPPSRVLPEPTPLPLPPTRPLPLPVPLASLIGRDSEVALI
                     TNLLLRPDVRLVTLTGPGGVGKTHLAMHIAPLLADQFADGATVMELASLRASELVLPT
                     LARRLGVRPAKDAPLLERTIDALRAQELLIVADNLEHVMEAAVDFARLLAACPGLKIL
                     ATSRVRLHITGEHTLDVPPLSLPEVAGPVSVADLAASDAVRLFVSRAEAARATFALTA
                     ENAPAVAELCRRLDGLPLAIELAAGQCDVSSPEELLARLDQRDLPLDAGRRDAPARHQ
                     GLDAAIDWSYDLLSPSEQHAFARLAIFAGSFTVLAALAILGGDELEGTPLSLVSALVA
                     KSLITRTENRSGGSIFRMLQVIRDYALRRLEADPDAPELYRRHARYFATRAEQWAADI
                     QGADLAEWSANVEYEVDDMRAALIWAIEHDRATAARMSTALVPIWAARGTMTDGRAWL
                     ERALANAEQNDLASVGPNGDGPRPPSRTRSTVRLTAREEQVLRLLVDGLSDKEIAAAL
                     SISARTVSNHVSGLLAKLGVTSRTAAATLALREGTLEEG"
BASE COUNT          818 a         1674 c         1583 g          784 t
ORIGIN      
        1 ctaccgcgcc gagctgacct tcccgcaggc cgggagctgg aagtggatga tcacgcccga
       61 gcccttcgcc ggcacctcgt tcgcctcgat ccaggtgcag gatgccgtcg gctcgaccgc
      121 atcggacgca tccgccccgc acccggcaag catccgcgcc ggcagttgcc aggcgctcgg
      181 cgagaccagc ttcccgctca gcgacgtgct cccgaacggc gtcgtcctcg atggcaagac
      241 cgatcagacc accggtgtgg tcggcgtcga tgccggcgtg ccggtcgcga tcagctcaac
      301 gacggtcgat gtcacgatcg ccgagctgct ggccgagccg cacgcgatca gcgtggtcaa
      361 gcaggcagat gatgccggca cggaggtcgc ctgcggcaat atcagcggcc ggatctggaa
      421 cgacgagctg atcgtcggcc tgcagcagcg caacaactcg agcgatatcg gcatcgccgt
      481 cctgcgcgag gcggccggca agacgacggt ctcgctctac atgatggtcg tcgccaccga
      541 gcaggctgcc gcgaccgggc cggaggtgac cgtcgagatc accggcagct cgggcgactg
      601 gcacttcgag ccggcccggg tcgagatcgc gcccggcacg acggtcgtct ggatcaacag
      661 gaccgagacc tcgcacacgg tgaccggcac ggacctcgcc ttcgaggact ccagcccgat
      721 cgcgcccggc gggacgttca gcgaagtctt caccgatccc ggcgagttcc ggtacttctg
      781 ctcgccgcac ccgttcatga ccggcgtggt cgtcgtcacc gagccggggt catgaggccc
      841 cggcgtccgg cttcgggttg cgcggcgcga agctgatcag cgtcccgatc acgaggaagc
      901 cgatcaccag cgtttgggcc agcgtcgacc agtccgattg ccaaacccag gtcgcgacgg
      961 cccacggcag gagcaggacg ccgacgatga tctgaacgac cagattccag tcgaggtact
     1021 ggtcccacat gcgctgcagg gtatccggcc agaagaggac cagcgccccg aacgcgaccc
     1081 agagcaggaa gacgccgccc acgccgccgt agggactgac ctgggcggcc tgggtgcgtc
     1141 gcgaccttgg ttcggatggt gaatacgatc cggacggcat ggcgtccagt ggatgattgg
     1201 ccattgctcg ctcccttcca gagcggcgcg gtcagtgcgt cgcgcgggtc agatgctggc
     1261 gccggagggc gtacgggatc gcgccgaaga tcatgccgat cgtgaccgcc gcggcccaga
     1321 acgccgcccg ccggagcggc atccgcaccg acagggcatc ggcgaagacg agcgtcgcga
     1381 acagcaacat cgtggcaaag aagccgtagg cgactgccga tcccacgatc aggtcgaatg
     1441 cccgtccgat caggaagagc gcgacctgca cgacaaccag cgacgtggcg atcacccgga
     1501 ccggctcgcg atcaaagtcg atcgagaacc agtggctccg gtcgagctgt gaattgggat
     1561 gtgaaatcat catcgtgcgc acgccgcttc tcctgcgtca ttggcgccgg gatgagcatg
     1621 gcggcccaat gccaggcagc atctcccggc ctcgcctcca tcatccgctg ccaggcatgc
     1681 catcgtcgct gagattcagg cacacgcgtt gcaaattcgt ggcaagcggc caatgcatcg
     1741 cgcatatatc ggaaacggcc ggacatcgta gcgccgttac aacgccccgc cggtctcttc
     1801 gggagaccgg tgttgcatct gtagctcggt gtcccggcgc cgcccggtgc taaaggaccg
     1861 ggctgagtct gaataaggcc cttcgggact ccaaaaaaga gagtcggctt tagccgcctt
     1921 atattggttc agccccggcc ttcagtctgg gcggtcaggc cggtcgcgca accagcccct
     1981 ttagtgggct tctcgtggtt tggcccaggc atttatcccc gggcggcacg gaccgtgttt
     2041 catccggaga tgcgacgctc gctcgaccgc tgcgctctgg cggcatgcct atttcgtgac
     2101 cggtctccgc tccatgcgca tgtctcgacc gccccgaaaa cgtagcgcgg ggttaccatg
     2161 ccccgcttgt ctcatcccat gcacggaggc aagaggagcg cggttgacca cgttggttcc
     2221 cgcgccgaac ggatcggcag cgggtccggt cggggcatgt tcctctctcc ggttctctcc
     2281 tcaaggaaag agggcatccc cgcgctacgc caaggacgcg aatagtgcgt tagggctcgg
     2341 gcgcggaggc gcggtggatc tcggtagaga tctcctggat gcggcggagg agatcgatct
     2401 gctcccgttg cagggccacc agggtgtccc attgccggcc gccgagctca tccaggcggt
     2461 tctggatcgc ttcgatctcc tcctctgcct ggcgatcgac ggcgtaatcc tcgaccgcct
     2521 ccagccgatc ccgggcggcc tgccggttct ggctcatcat gatgatcggc gcggtgaagg
     2581 cggcctggaa cgagaggacg aggttgagca ggatgaacgg gtacgggtcc cagtgccgga
     2641 tccaggccag gctgttcacg atcagccaga tcgcgaggat gccgctctgc accaggatga
     2701 aggtccacga gccgacggat gtggtcacca catcggccgc gcgttggccg agggtcatcc
     2761 gctcggcggc gaagcgttcc tcaggcgagc ggggctcgcc caggcggatc agcgccgctt
     2821 ccagcgcgcg ccgctccagc ggggtcgggg gctcgccctc ctgccgcagg atctcgctca
     2881 gacgctccgc catgcgcgca tgcctccacg gggatcatag gagatcgcgc gtcgaattcg
     2941 gattgtcaaa cctctgcgag attcgccagg aagctatcgc gtggtgcgga aatggcccgt
     3001 tgcatgggac aagttgaccg tctcaaccgt tgacaccgca aaatacgatg gcatgatacg
     3061 aacacgttca caggcgatcc gcaccggagg gcccaatgtc ggaccctatc ccaccgagtc
     3121 gggtgcttcc tgagcccact ccactgccat tgccccctac gcgcccgttg ccgctgccgg
     3181 ttccgctggc atccttgatc ggccgggact ccgaggtcgc cctgatcacc aatctactgc
     3241 tccggcccga tgtccggttg gtgaccctga ccggtccggg cggtgttggc aagacgcacc
     3301 tggcgatgca catcgcgccg ctgctcgccg atcagttcgc tgacggcgcc accgtcatgg
     3361 agctggcttc cctgcgcgct tcggagctgg tgctgccgac cctcgcccgc cgcctcggcg
     3421 tgcgcccggc caaggacgcg ccgctcctcg agcggacgat cgacgccctg cgcgcgcagg
     3481 agctcttgat cgtcgccgac aacctcgagc acgtcatgga ggccgccgtg gacttcgcgc
     3541 ggctgctggc tgcttgtccc ggcctcaaga tcctggccac cagccgggtc cggctccaca
     3601 tcacgggcga acacacgctg gatgtgccgc cgctctcact gccggaggtg gcaggccccg
     3661 tgtcggtggc cgatctcgcc gcctccgatg cggtccggct tttcgtcagc cgcgccgaag
     3721 cggcccgcgc caccttcgcg ctgacggcag agaacgctcc tgcggtcgcc gagctctgcc
     3781 ggcggctgga tggcctgccc ctggccatcg agctggcggc gggccagtgc gatgtcagct
     3841 cgccggagga gctcctggct cgcctggatc agcgcgacct cccgcttgac gccggccggc
     3901 gcgatgcccc cgcccggcat caggggctcg atgccgccat cgactggagc tacgatctgc
     3961 tctcgccgtc cgagcagcat gcgttcgcgc gcctcgccat cttcgccggc tcgttcacgg
     4021 tactggccgc actggcaatc ctgggtggtg acgagctcga aggcactccg ctcagcctgg
     4081 tctcggcgct cgtggccaag agcctgatca cccggaccga gaaccggtcg ggtggctcga
     4141 ttttccgcat gctgcaggtg atccgcgact atgccctgcg ccgcctcgag gccgatcctg
     4201 atgcgcccga gctctaccgc cgccatgcgc gctacttcgc aacccgggcc gagcaatggg
     4261 ccgccgatat ccagggtgcc gacctcgccg aatggagcgc gaacgtcgag tatgaagtcg
     4321 atgacatgcg tgccgcgttg atctgggcga tcgagcacga ccgggcgact gcagcccgga
     4381 tgagcaccgc gctggtcccg atctgggccg cgcgggggac catgactgac ggccgcgcct
     4441 ggctggagcg ggccctggcg aatgccgagc agaacgatct ggcctccgtt gggccgaatg
     4501 gcgatggccc gcgcccgccg tccaggaccc gttcgaccgt gcgactgacc gcccgcgagg
     4561 agcaggtcct ccgattgctg gtcgatggct tatccgacaa ggagattgcc gccgcgctct
     4621 cgatcagcgc ccgcaccgtc agcaatcacg tcagcggcct gctcgccaag ctcggggtca
     4681 cctcccgcac cgccgccgcc acgctcgccc tccgcgaagg caccctggaa gagggttgag
     4741 gggacagggg acagtggaca ggggacaggg gttagaaggg atagggagcc aatcccaaat
     4801 cccaaatccc gaaaaccgaa ccccgaaaac ctagaaccta aaacccaaaa cccaaaacc
//
LOCUS       DATAOZ010000183         7220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8757126, whole genome shotgun sequence.
ACCESSION   DATAOZ010000183 DATAOZ010000000
VERSION     DATAOZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8757126"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VHR64_04920"
     CDS             <1..843
                     /locus_tag="VHR64_04920"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3302202.1"
                     /translation="PTSAAQATAIGKPAPDINLPDLKGNPVTLADFAGSPTAVIFWNP
                     GCGFCRKMLDDLKAWETERPAGSPQTLLISTGSIDANKEQGIASTVVLDEGFATGRAF
                     GVSGTPSAVLVDEHGLIASAPSVGGPRVMAMLTGEEIVPDAPATPSVVLQEGDDAPPI
                     SLPDLDGNTVSLSDFKGTETLVLFWNPGCGFCQRMVNDLRAFEANASASSPRLLLIST
                     GDIEANRQAGLQAPILIDHGFATGRAYGANGTPSAVLVDANGKIAGPLLVGAPNILAA
                     SGHV"
     gene            1247..1543
                     /locus_tag="VHR64_04925"
     CDS             1247..1543
                     /locus_tag="VHR64_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302203.1"
                     /translation="MSSSNDEEGANWSHIEPSREFRVEYRVAGSETVVLARVTNVFPH
                     HDTLTQYVSRFLSECGASTPRGELVLVDDDTNAVLARRDLYRAFHDFQKARNLT"
     gene            complement(1773..1967)
                     /locus_tag="VHR64_04930"
     CDS             complement(1773..1967)
                     /locus_tag="VHR64_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302204.1"
                     /translation="MNDPRNLDSEAKPEIKTEQEHYPPRRYDDPHTVAEEEKRFGMSM
                     WIGIAIIGILFLIVLLIFAL"
     gene            2120..2200
                     /locus_tag="VHR64_04935"
     tRNA            2120..2200
                     /locus_tag="VHR64_04935"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2153..2155,aa:Leu,seq:taa)
     gene            2221..2511
                     /locus_tag="VHR64_04940"
     CDS             2221..2511
                     /locus_tag="VHR64_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX3302205.1"
                     /translation="MTTKPNDTIGNTGLRVTVDPRRCVANGKCTYAAPGVYVIDEETG
                     IAYVDDEERATVENLFAGARACPTDAIIIEQYGRRVYPRILAPNRESNVAGE"
     gene            complement(2608..4233)
                     /gene="groL"
                     /locus_tag="VHR64_04945"
     CDS             complement(2608..4233)
                     /gene="groL"
                     /locus_tag="VHR64_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX3302206.1"
                     /translation="MIEFNEQARQSLKQGVDVLANAVKTTLGPKGRNVALDKKFGAPT
                     VTHDGVTVAKEIELDDHFANMGAQLLKEAATKTNDVAGDGTTTATVLAQAIVHEGMRN
                     VAAGANAMLLKRGIELGTDALIEKIQEMAVAVKGKEEIAQVAAISAADPEIGSLIAEV
                     MEKVGKDGVITVEESKGLLFETEYTEGMQIDRGYLSPYFVTNTERMEAELDDPYILIT
                     DKKVSSIQDILPVLEKVATARAPFVIIAEDVDGEALATLVVNKLRGTLNVLAVKAPGF
                     GDRRKEMLRDIAILTGGQVISEEVGRKLDTAGIEDLGRARRVVATKDDTTFIEGHGDE
                     KAIKGRVEQIRAQIETTTSDFDREKLQERLAKLSGGVAVIKVGAATETELKEKKHRVE
                     DALSATRAAVEEGIVPGGGVALINALSALDSVQAEGDILTGVKILRRALEEPLRGIAA
                     NAGKDGAVIIEMVRQKAADKKNANIGYDVISDEYVDMISAGIIDPAKVTRSAVENAAS
                     IASMILTTEALITDKPEEKAPAMPPGGMGGMDY"
     gene            complement(4286..4594)
                     /gene="groES"
                     /locus_tag="VHR64_04950"
     CDS             complement(4286..4594)
                     /gene="groES"
                     /locus_tag="VHR64_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX3302207.1"
                     /translation="MGSTAVEVKLRPLGDRVLVKPLGREETTKSGIVLPDTAKEKPQR
                     GTIVAVGAGRLDDDGDRIPVDVEVGNEVLFAKYAGTEFKLDDEEYLILAEKDILAVLG
                     "
     gene            4870..6063
                     /locus_tag="VHR64_04955"
     CDS             4870..6063
                     /locus_tag="VHR64_04955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3302208.1"
                     /translation="MHAAHRSPAEFLAARVRQAAPSPTLAVSEKARQLKAQGRDVIDL
                     GGGDPDFITPEHIRIAAADAMNAGDTHYVASNGTPALRNALAMKLKDDNGLTYDPSQI
                     IVTTGGKQALFEASMALVEPGVDVLILEPAWVSYDPMVTLAGGNVIHVGLDPDTNWTV
                     TREALEAHVTPASRILFMNSPCNPTGRVLTKEEHQTIADFATDHDLIVFTDEMYEKII
                     YDGRTHFSLATWPGLWERTITFNGFSKAYAMTGWRLGYMAGPKVFMNEIAKVQSHSIT
                     HATSFAMAGAVAALNGPQESIGEMVRAWDRRRTLVTDGLNRIKGISYAAAEGAFYAFP
                     DIRGTGMSSMEFSDKALTEAEVAVVPGNAFGDAGEGFVRMSFATSDEILQKMIDRLSN
                     WLGTS"
     gene            6073..6522
                     /locus_tag="VHR64_04960"
     CDS             6073..6522
                     /locus_tag="VHR64_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3302209.1"
                     /translation="MQIERRLEELGLTLPSPVSPAANYVRYVQSGNHLYISGTGPSKD
                     QPSGKLGQEIDIETGYQVARDVGLQLIATMKDALGDLDRVKRVVKLLGMVNSTPDFSE
                     QPKVINGCSDLMVEVFGEKGRHARSAVGFAALPSQIPVEIEVIVEVE"
     gene            6629..7099
                     /gene="greA"
                     /locus_tag="VHR64_04965"
     CDS             6629..7099
                     /gene="greA"
                     /locus_tag="VHR64_04965"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX3302210.1"
                     /translation="MANQDRVPVTKEGLERLRAELEELKSVKMPQITEAVAEARSHGD
                     LRENAAYDAARHDQMMTQRRVDELEAMLRNSVVIDDAQIRAGVVGLGSRVTVDFDGDE
                     ETYTIVGAIEAKPTAGMISNESPIGQALIGKRVGQTAQVATPGGLTPIKILRVE"
BASE COUNT         1321 a         2282 c         2223 g         1394 t
ORIGIN      
        1 cctacatccg cagcgcaagc caccgcaatt ggaaagccgg cgccagacat caacttgccg
       61 gacctgaaag gcaatccagt gacgctggcg gatttcgcgg gcagtcccac ggccgtcatc
      121 ttctggaatc ctgggtgcgg gttctgccgt aagatgctgg acgatctcaa ggcatgggag
      181 actgagcgac cggccggatc gccgcaaaca cttctgatct cgaccggctc catcgacgcg
      241 aacaaggaac agggcatcgc ctcgacggtt gtgctggatg agggattcgc gacgggccgc
      301 gcgttcgggg tgagcggtac gccctcggcc gtgctcgtgg acgaacacgg tctgatcgcc
      361 agcgcgccct cggtcggcgg accgcgcgtg atggccatgc ttactggcga ggagatcgtg
      421 cctgacgctc cggcgacgcc gagcgttgtt ctgcaggaag gagatgacgc tccgccgatc
      481 tcactgccgg acctggacgg gaacaccgtc tcattgtccg atttcaaggg cacagagacg
      541 ctcgtcctgt tctggaatcc tggctgcggc ttttgtcagc gcatggtcaa cgacttgagg
      601 gcattcgagg ccaatgcgtc ggcaagttcg ccgcgcctgc tactgatttc gaccggggat
      661 atcgaagcca atcgccaggc tggattgcag gccccgatcc tgatcgacca tggcttcgcg
      721 accggacggg cgtacggcgc caacggcacg ccatccgcag tcctggttga cgcaaacggg
      781 aagattgcgg gtccgctgct ggtcggggcg ccgaacatcc tcgcggcgtc cggacacgta
      841 tagtccacgg acctccgcgg gtttcgacga tggctggtgg tcaaatgaaa ccaacgcgcc
      901 tccgcacact actgccatgc ccaacacatg ccgctgctcc gcaaggaggc gttccttccg
      961 tttcgacggc aggtagccgg gccaacgtct acccgaacac ccctggcacg gcccctcttt
     1021 ggcagagatg tagcgcctcc tactgcgatc tccctatgca agaccaaagt accgcgtttg
     1081 gtgcgacaga tgggctacac attctcctcg tgcctccccc tactttcggg ctattcctta
     1141 cgtacgcacc cggaacgcca attggccgta caggtgcgag aggtaaggac acgcgagccg
     1201 gtgatcgcaa gacacagact cgcatagcgg tggactgatg ggtctgatga gctcgagtaa
     1261 tgacgaagag ggcgcgaact ggtcacacat cgagcccagc cgcgagttcc gggttgagta
     1321 ccgggttgcg ggttcagaaa cggtcgtgct ggcgcgggtc acaaatgtct tcccgcacca
     1381 cgatacgctg acgcaatacg tttctcgctt cctctcagaa tgcggtgcct cgacccccag
     1441 gggcgagctg gtgctcgtcg acgacgacac caatgccgtg ctcgcgcgac gcgatctcta
     1501 ccgcgccttc cacgacttcc aaaaggcacg caatctcacc tgattctcgg ctggatcgaa
     1561 taatcttcgt cgccaatctc atcacgaatt gctgaaggag ctcccgctct gccgagcgtc
     1621 agtaatcatc tcgccaatac agctacgagc tacagcgata tgcgcaaaga tcggccgcct
     1681 tcgtaacgta gcgcggggtt accacgtccc gcattggtga tccccgcgct tggtccattg
     1741 cgccgagtcg tagcttgcat gctgcgacgc tgtcacagcg cgaatatcaa cagcacaatc
     1801 aggaagagaa tgccgatgat cgcgatgccg atccacatcg acatcccgaa gcgcttttct
     1861 tcctccgcaa cggtgtgcgg gtcgtcatag cgtcgtggcg gataatgctc ctgctcggtc
     1921 ttgatctcag gttttgcttc ggagtccagg tttcgcgggt cgttcatggc tgcacctcct
     1981 gccagcgaaa cagcgttttc gcctggccaa cgcgtgtcac cttaccgttt cgctgaaggc
     2041 cgggggcgtg aggacgttac accgctggcg aaacgaagaa cctgcccggt cattgggtag
     2101 acttgcggtg acattcgctg cggatgtggc ggaacggcag acgctgcgga cttaaactcc
     2161 gctggggtaa cccgtgcggg ttcgaatccc gccatccgca cactgagagg agattctctc
     2221 ttgaccacca agccaaatga cacgattggc aataccggcc tgcgcgtgac ggtcgacccg
     2281 cgccgctgcg tcgccaacgg caagtgcacc tatgcggcgc cgggcgtcta cgtgatcgac
     2341 gaggaaaccg gcatcgccta cgtggatgac gaggagcgag cgaccgtcga aaacctcttc
     2401 gccggcgccc gcgcctgccc aaccgacgca atcatcatcg agcaatacgg tcgccgcgtt
     2461 tacccccgca tcctcgcgcc gaatcgagaa tcaaacgtgg cgggcgagta gtaccggcct
     2521 tttgccggtt ttgctgaatg agaaacgggg ccggtggtag ccaccggccc cgtttctttt
     2581 gtgtcgcgtg ttctgggcgt tttcggacta gtagtccatg ccgcccatgc cgcccggagg
     2641 catggccgga gccttctcct cgggcttgtc ggtgatgaga gcctcggtgg tgaggatcat
     2701 cgacgcgatc gacgccgcgt tctcaaccgc ggagcgggtg accttggccg ggtcgatgat
     2761 gccggcgctg atcatgtcca catactcgtc gctgatcacg tcgtagccga tgttggcatt
     2821 cttcttgtcg gcggccttct ggcggaccat ctcgatgatg accgcgccgt ccttgccggc
     2881 gttggccgcg ataccgcgca gcggctcttc gagcgcgcgg cgcaggatct tgacaccggt
     2941 aaggatgtcg ccctcggcct gaacgctgtc gagcgcggag agcgcgttga tcagcgcaac
     3001 gccaccaccc ggcacgatgc cttcctcgac cgcggcgcgc gtggcgctca gggcgtcctc
     3061 aacgcggtgc ttcttctcct tgagctcggt ctcggttgcg gcgccgacct tgatgaccgc
     3121 gacgccaccg gagagcttgg ccagccgctc ctgcagcttc tcgcgatcga agtcggaggt
     3181 cgtggtctcg atctgggccc ggatctgctc gacgcgaccc ttgatcgcct tctcgtcgcc
     3241 atgaccctcg atgaaggtcg tgtcgtcctt ggtggcgacc acgcggcggg cgcgaccgag
     3301 atcctcgatg ccggcggtgt cgagcttgcg cccaacttcc tcggagatga cttggccacc
     3361 ggtcaggatg gcgatgtcgc ggagcatctc cttgcggcga tcaccgaagc ccggcgcctt
     3421 gacggcgagg acgttgaggg tgccgcggag cttgttgacg accagcgtgg ccagggcctc
     3481 gccgtcgacg tcctcggcga tgatgacgaa cggcgcgcga gccgtggcga ccttctcgag
     3541 caccggcagg atgtcctgga tcgaggagac cttcttgtcg gtgatcagga tgtacggatc
     3601 atcgagctcg gcctccatcc gctccgtgtt ggtcacgaag tacggggaga ggtaaccgcg
     3661 gtcgatctgc atgccctcgg tgtactcggt ctcgaaaagc aggcccttcg actcctcaac
     3721 cgtgatcacg ccgtccttgc cgaccttctc catgacctcg gcgatcagcg agccgatctc
     3781 ggggtcggcg gccgaaatcg ccgcgacctg ggcgatctct tccttgccct tgaccgcgac
     3841 ggccatctcc tgaatcttct cgatcagggc gtccgtgccg agctcgatgc cgcgcttgag
     3901 cagcatcgcg ttcgcgccgg cagcgacgtt gcgcatgccc tcgtgaacga tcgcctgggc
     3961 gaggacggtc gccgtcgtgg tgccgtcacc ggcgacatca ttggtcttgg tcgcggcctc
     4021 cttgaggagc tgcgcgccca tattcgcgaa gtgatcgtcg agctcgattt ccttggcgac
     4081 ggtgacaccg tcatgcgtga cggtcggggc gccgaacttc ttgtcgagcg cgacattgcg
     4141 gcccttcgga ccaagggtgg tcttgaccgc gttggcgagg acatcgacac cctgcttcag
     4201 ggactgtcgc gcctgctcgt tgaactcaat catctttgcc actgtcagtt tctccgttgc
     4261 gtgtgattcg tattgccgaa acgaattagc cgagaaccgc gaggatgtcc ttctccgcca
     4321 ggatcaggta ctcctcgtca tcgagcttga actccgtgcc cgcgtacttg gcgaacagga
     4381 cttcgttgcc gacttcgaca tcaaccggaa tccggtcgcc atcgtcatcg agccgacccg
     4441 cgccaaccgc aacgattgtt ccgcgctgag gcttctcctt ggcggtgtcc gggagaacga
     4501 tgccgctctt ggtcgtctct tcgcgaccaa gcggcttgac caggacacga tcgcccaggg
     4561 gccgcagttt gacttccact gctgtcgaac ccattgctaa ttcaacctcc ttcggttgat
     4621 ccttgattgc cgcggcgcgc acgcgcagac ggcaacaccg tttcgtagcg tctggaatgg
     4681 tagcagtcat tttagcactc tgcaagtggg agtgctaaat atcaaacaga cgcattatcg
     4741 cgcccattca cccgacgccg cgagcggcga tggtagactt tccggacgtc gagtcatcga
     4801 cttttctggc ttttcaccca ttcagacgca cgttcgtgat gacagtcacg acagtcgtgg
     4861 gaggactccg tgcacgctgc tcaccgctct cccgccgagt ttctcgcggc acgtgtccgc
     4921 caggcggctc cctctcctac cctcgcagtc tccgagaagg ctcggcagct caaggcgcag
     4981 ggtcgggatg tgatcgatct cggcggtggc gatccggact tcatcacccc ggagcatatc
     5041 cgcatcgcgg ctgccgacgc gatgaacgcg ggtgacacgc attacgttgc cagcaacggc
     5101 acgccggcgc tccgcaatgc gctcgcaatg aagctcaagg acgacaacgg gctgacctat
     5161 gacccgtccc agatcatcgt caccaccggc ggcaagcagg cgctcttcga ggcgtcgatg
     5221 gccctggtcg agcccggcgt cgatgtcctg attctggagc cggcctgggt ctcgtatgac
     5281 ccgatggtga cgcttgccgg cggtaacgtg atccacgtcg gactcgatcc ggatacgaac
     5341 tggacggtca cccgcgaggc gcttgaggcc cacgtgacgc ctgccagccg gatcctgttc
     5401 atgaactcgc cgtgcaaccc gaccggccgc gtgctgacca aagaggaaca ccagacgatc
     5461 gcggatttcg cgaccgatca cgacctgatc gtcttcaccg acgagatgta cgagaagatc
     5521 atttacgacg gccgcacgca tttttcgctg gcgacctggc cggggctttg ggaacggacg
     5581 atcactttca acggcttttc gaaggcctac gcgatgaccg gatggcgact tggatacatg
     5641 gccggaccga aggtcttcat gaacgagatc gccaaagtcc agagccattc gatcacccac
     5701 gccacgtcgt tcgcgatggc cggagccgtc gcggcactca acggtccgca ggagtcgatc
     5761 ggcgaaatgg tccgggcgtg ggatcgccgg cgcacgctcg tcaccgacgg gctgaacaga
     5821 atcaaaggca tctcctatgc cgcggccgag ggcgcgttct acgcattccc tgacattcgc
     5881 ggcaccggaa tgagctcaat ggagttctcc gacaaggcgc taaccgaggc cgaggttgcg
     5941 gtcgtgccgg gcaatgcctt cggcgacgcc ggtgagggct tcgtgcggat gtcctttgca
     6001 accagcgacg agatcctgca gaaaatgatc gatcgattga gcaactggct cggaaccagc
     6061 taggagtcat gcgtgcagat tgagaggcgc ctcgaagagc tcggtctcac ccttcccagt
     6121 ccggtctctc cggctgccaa ttacgtacgt tatgtgcagt ccgggaacca cctgtacatt
     6181 tctgggacgg gcccgagcaa ggatcagccg agtggcaagc tcggccaaga gatcgatatc
     6241 gagaccggat atcaggtggc gcgcgacgtt gggctgcagc tcattgcgac gatgaaggac
     6301 gcgctcggcg atctcgaccg ggttaagcgc gtcgtcaagc tcctcggcat ggtcaactcg
     6361 acgcccgact tcagcgaaca gccaaaggta atcaacggct gctccgatct gatggtcgag
     6421 gtcttcggcg agaagggccg gcacgcccgc tcggcggtcg gcttcgcggc acttccctca
     6481 cagattccgg tcgagatcga ggtcatcgtc gaggtcgagt agcgctccct cccgtgtacg
     6541 gtcagtatcc gcgaatagga tttgtcacat ctcccgggcg cagtgattgc tcccgctcgg
     6601 gaagacatta atgaaaggat cggcaggcat ggcaaatcag gatcgggttc cggtgaccaa
     6661 agagggcctg gaacgactcc gtgccgagct agaagagttg aagtccgtca agatgcctca
     6721 gatcaccgaa gcggtggcag aggcgcgctc gcacggcgac ctgcgcgaga acgcggccta
     6781 cgacgccgcg cgacacgacc agatgatgac ccaacggcgg gtcgacgagc tcgaggccat
     6841 gctccggaat tcggtcgtga tcgacgacgc gcaaatccgg gccggggtcg tcggtctcgg
     6901 ctcgcgcgtc accgtcgatt tcgatggcga cgaggagacc tatacgatcg tcggcgcaat
     6961 cgaagcaaag ccgacggccg gcatgatctc gaacgagtcg cctatcggac aggccctgat
     7021 cggcaagcgc gtcggtcaga ctgcgcaggt cgctacgccg ggcggcctga ccccaatcaa
     7081 gatcctccgt gtcgaataat cgcgctcgcc tgtgaactgg cttgatcggt ggcgggaacg
     7141 gcgcggcgct cgtcggcggg atggcgccta tcgcagactc tggcaggcgt tcacccgcta
     7201 tccgacactg gccgacggcc
//
LOCUS       DATAOZ010000184         6766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7179208, whole genome shotgun sequence.
ACCESSION   DATAOZ010000184 DATAOZ010000000
VERSION     DATAOZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7179208"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VHR64_04970"
     CDS             <1..731
                     /locus_tag="VHR64_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3302211.1"
                     /translation="DQEATQLFVRANLRLVVSIAKRYVNRGLTLLDLIQEGNIGLMRA
                     VQKYDWRKGFRFSTYATWWIRQAITRAIADQSRTIRLPVHMGDSISRYRKTLNTLAQE
                     LGRQPTPEEVAEAMAVAPEKIHQIVQAAQRTISLETPIGSEDETSLGDLIADEVSETP
                     YEAASESMLKRDVSAALDTLTPREKLVLQLRFGLGHSHQHTLAEVGEQLQISRERVRQ
                     IENEALQKLRRLDGDRLFAYHQEL"
     gene            833..2185
                     /locus_tag="VHR64_04975"
     CDS             833..2185
                     /locus_tag="VHR64_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX3302212.1"
                     /translation="MDSVLKLLAVFALVAINGLFVAAEFALVGARPTRIDQLAATGNR
                     WARFVKRAMDDPNRFISAAQVGITMASLGLGWLAEPAVAELLIPILEPILGEGGKIGA
                     HLVAGIIALFLITLLHIVLGEQVPKMIALQRAEPTILTIAPVISVIAVPFRPLIALLY
                     WLTSVVLKLLHLEWQGEHHLVYSEDELKLMITASHQKGILDENEEAMINRVFGFAEMV
                     TEQVMVPRTEIEAIPIRATLDEVVKVVADSGHSRYPVYGENLDDLRGVFHAKDLYTLL
                     SRNEQHRFSLRRMVREPLMVAESTNLDDLLTRMKRERTQIAIVVDEYGGTAGMVTLED
                     VLERIIGDVQDEFETAGDDVVILEEGCAEISGLLSLEDVNERFDTHFEDSYYNTIGGF
                     VFGQLGRRPEVGDTVTIEKVDLTVLRLDGLRIDRIKINLPPIDEPITQEEPVPVGEAG
                     "
     gene            complement(2394..3656)
                     /locus_tag="VHR64_04980"
     CDS             complement(2394..3656)
                     /locus_tag="VHR64_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302213.1"
                     /translation="MAAITATIDSRTETLSREQLDFGFRVLAITTFCMTLALSSFDSI
                     QANFFRDSLGMDGELNGYLIAIREVPGFLLIFIAAMMLRRGLSTATAVSIFIAGVGFS
                     LFAFTHSFSQAIIPILVCSIGYHSWLQLQYALGLSLARNGEEGTTLGKLQGIGFLGTF
                     LALVLVWGVLQSAEWLTGSDHQDGVLRIFWLFAGAVAIIGAVVIRRFPQSARDRREAR
                     VAPRLTWRREYWLYYVLATLDGSRQQIYFAFAPFVLVERFNVPARHLIVVLIIAALIK
                     WRLGEVIGKMVDRHGEKMTLNVAYVLHLIVFLGFALSPNVWFAYVCYLGYNFLSLFSI
                     GTTTYIKKIARREDLTPSLAMGVSLAHVTAIVVPVFGAALWDQLGYRFPFLFGTIFVI
                     LSLVFTQKIDVAKQRIGTWGMAEPAAAD"
     gene            complement(3706..4971)
                     /locus_tag="VHR64_04985"
     CDS             complement(3706..4971)
                     /locus_tag="VHR64_04985"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3302214.1"
                     /translation="MAEAGKPHVVIIGGGFAGLNAAKQLRDADVAVTIVDQHNHHTFQ
                     PLLYQVATASLSPVDIAGPIRGILRSQKYLRVILDEVTGIDLARSVIQMGDDELGFDY
                     LIIGAGSTHSYFGHDEWEKYAGGLKTLDDAVRMRNQILLGFEAAERAHSDDERRREMT
                     FAIVGGGPTGVELAGAIAEIARHSISRDFDVIDPRQAKILLIDAGPRLLLAYPESLSK
                     HAQTDLERLGVDVRLNALVTGVDAHGVTLKSGERIEAATILWAAGVQASPLGRTLGTE
                     LDRAGRVVVNPDLSVPGHPNVFVAGDLAALNGKDGKQLPGVAQVAMQQGRAAAKNIVR
                     RVAAEPTEPFRYKDLGNMATIGRNRAVADIHGIRLHGFIAWVIWLTIHLVNLIGFRNR
                     FVVMTQWVWSYFTFYRRVRLITRPGTVTD"
     gene            complement(4989..5279)
                     /locus_tag="VHR64_04990"
     CDS             complement(4989..5279)
                     /locus_tag="VHR64_04990"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX3302215.1"
                     /translation="MTIGVAQITLYLEAADSLKDKRRVVKSLIQRIHNRFNVAAAEVA
                     DLDVHRVATIGVAVVSNSGAHCSDILAAVIAFVDGNVELGSMGEVETELIPY"
     gene            complement(5276..5743)
                     /locus_tag="VHR64_04995"
     CDS             complement(5276..5743)
                     /locus_tag="VHR64_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302216.1"
                     /translation="MADDFQEDIGAEFDVEDELELLRSNTLAMALGTISFLRIQGIPA
                     TEWAKSLGEIFARGWDTDEAWSPEDFLDATIVNLTAFGGEAVQAEFSEDEATAMIADF
                     PDPARVEGLGLDNVEGDLLYDLIAPIALACGVWYHWERHGEHVRITIRPAPVL"
     gene            5880..>6766
                     /locus_tag="VHR64_05000"
     CDS             5880..>6766
                     /locus_tag="VHR64_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3302217.1"
                     /translation="MPANTYDVVIVGGGHNGLVAACYLAKAGHSVCVLERYHEVGGAA
                     FTQEIHPGYLLSEGSYVLSLMPRKIIDELGVTDDIQLVKRNPRFFMPFPDGQSLTAWE
                     DDAKFLDEIRKFSKKDAENYPRYDAFVEKASEVMDQFILRQPPTFAEFASQFKTTDEA
                     RIFQKMILGSAAEVAEYFFESEQLQANAVAFGLIGTFRGPRDAGTAYVKLYHAMGNVT
                     GRRGQWAYVKGAMGAVSQAYKRVALANGVTIRTSAEVAQICVENGRATGVVTTDGEDF
                     HSSVVFSNADPQRTYLKLTG"
BASE COUNT         1313 a         2190 c         2103 g         1160 t
ORIGIN      
        1 acgaccagga agcaacgcag ctcttcgtcc gggcaaacct ccgtctggtg gtctcgatcg
       61 ccaagcgcta tgtgaatcgc ggtctgaccc tcctggactt gatccaggaa ggcaatattg
      121 gcctgatgcg cgccgttcag aagtacgact ggcgcaaggg cttccggttc agcacctatg
      181 cgacgtggtg gatccgacag gccatcaccc gcgctatcgc tgaccagagc cgcacgatca
      241 ggctccccgt tcacatgggc gattcgatct cccgctaccg caagaccctc aataccctcg
      301 cccaggagct cggccgccag ccgactccgg aagaggttgc tgaggcgatg gcggtggcac
      361 cggagaagat tcatcagatc gtgcaggcgg cgcagcgcac gatctcgctg gagacgccaa
      421 tcggtagcga ggacgagacc agcctcggcg acctgatcgc cgacgaggtt tccgagacgc
      481 cgtacgaggc cgccagcgag tcgatgctca agcgcgacgt cagtgccgcg cttgatacgc
      541 tcaccccgcg cgagaagctc gtgttgcagc tacggtttgg cttgggccac agtcaccagc
      601 acacgctggc tgaggttgga gaacagctcc agatcagccg cgagcgcgtg cgccagatcg
      661 agaacgaggc gctgcagaag ctgcgccggc tcgacggtga ccgcctgttt gcctaccacc
      721 aggagctcta gcactggatc ttcccagtca acctatcaat tactcaggcc agccctcagg
      781 gctggcctgc tttgctgcgc gcaggaggat gcgcgcccgc tagggcaaac cgatggatag
      841 cgttctgaaa ctgctcgccg tcttcgccct ggtggcgatc aacggcctct tcgtggccgc
      901 ggaattcgcg ctggtcggag cccggccgac ccggatcgac caacttgcgg cgaccggaaa
      961 ccgctgggcc aggttcgtca agcgcgcgat ggacgacccg aaccggttca tctccgccgc
     1021 gcaagtcggc attacgatgg ccagtcttgg tctcggttgg ctggccgagc cggccgtggc
     1081 tgagcttctg atcccgatcc tggaaccgat cctcggcgaa ggcggcaaga tcggcgcgca
     1141 cctcgttgcc ggcatcatcg cgctcttcct gatcacgctc ctccacatcg tgctcggcga
     1201 gcaggtgcca aagatgatcg ccctgcagcg cgccgaaccg accatcctga ccatcgcgcc
     1261 ggtgatctcg gttatcgcgg tcccgttccg gccgttgatc gcgctgctct actggctgac
     1321 ctcggtcgtc ctgaagctgc ttcacctcga atggcagggc gagcatcacc tcgtctactc
     1381 cgaggatgag ctgaagctga tgatcaccgc cagccaccag aagggcatcc tcgacgagaa
     1441 cgaggaggcg atgatcaacc gggtcttcgg cttcgcggag atggtcacgg agcaggtgat
     1501 ggtcccgcgc accgagatcg aggcgatccc gatccgggcg acgctggatg aagtcgtcaa
     1561 ggtcgtcgca gacagcggtc actcgcggta tcccgtctac ggcgaaaacc tcgacgatct
     1621 gcgcggtgtc ttccacgcca aggatctcta caccctgctc tcccgcaacg agcaacatcg
     1681 cttcagcctg cgccggatgg tccgtgagcc gttgatggtc gccgaatcga ccaacctcga
     1741 cgacctgctg acccgcatga agcgcgagcg gacccagatc gcgatcgtgg tcgatgaata
     1801 cggtggcacg gcgggcatgg tcacgctcga agatgtgctc gaacggatca tcggcgacgt
     1861 ccaggacgag tttgagaccg caggagacga cgtggtcatc ctcgaggagg gctgcgcgga
     1921 gatcagcggt ctgctctcgc tcgaagacgt caacgagcgg ttcgacacgc acttcgagga
     1981 ctcgtactac aacacgatcg gtggcttcgt cttcggtcag ctcggccgcc gaccggaggt
     2041 cggcgataca gtcacgatcg agaaggtcga tctgactgtg ctcaggctgg atggtctgcg
     2101 catcgaccgg atcaaaatca acctccctcc gatcgacgag ccaatcaccc aagaagagcc
     2161 ggtacccgtc ggcgaagccg gctaaccggg acctccaaat cctcgccgaa gccttggtga
     2221 cgtagcgcgg ggttaccacg ccccgcatcg ttgagcctca cgccggtccg gagaagccga
     2281 tcggcttccg caggcgattc acgttgacaa tcgaaacggt gtcggattcg ggcggggcgt
     2341 ggtaaccccg cgctacgaga tgtccgtcag caatcccacg agttgccaaa ggatcagtcg
     2401 gcagcggccg gctcggccat tccccatgtg ccgatgcgct gtttggcaac gtcgattttc
     2461 tgggtgaaga cgagcgagag gatcacgaag attgtcccga acagaaacgg gaaccgatag
     2521 ccgagctgat cccagagcgc cgcgccgaag acgggcacga cgatcgccgt cacgtgcgcc
     2581 agggagacac ccatcgccag cgacggggtc aaatcctcac gccgcgcaat cttcttgatg
     2641 taggtcgtgg tcccgatgct gaagagcgag aggaagttgt acccgaggta gcagacatag
     2701 gcgaaccaga cgttcggcga gagcgcgaag cccaggaaga cgatcaggtg aagcacgtag
     2761 gcgacattga gcgtcatctt ctcgccatgt cgatcgacca tcttgccgat cacttcgccg
     2821 agtctccatt tgataagcgc ggcaatgatc aggacgacaa tcaggtgacg ggccgggacg
     2881 ttgaagcgct cgaccagcac gaacggcgca aatgcgaagt agatctgctg ccggctgccg
     2941 tcgagcgttg ccagcacgta gtagagccag tactcgcgcc gccaggtcag ccgcggcgcg
     3001 acccgggcct cccggcggtc ccgcgctgac tgcgggaagc ggcggattac taccgcgccg
     3061 atgatcgcga ccgcgccggc aaagagccag aagatgcgca ggactccgtc ctggtgatca
     3121 ctccccgtca gccattcggc cgactgcagc acgccccaaa cgagtacgag cgccaggaag
     3181 gtgccgagga acccgatccc ctggagcttg ccgagcgtcg tgccctcctc gccgttccgg
     3241 gccagcgaga ggccaagcgc gtactggagc tgcagccagg agtggtagcc gatcgagcaa
     3301 accaggatcg ggatgatcgc ctggctgaac gagtgcgtaa aggcaaacag cgagaagccg
     3361 acgccagcga tgaatatcga taccgctgtg gcagtcgaca ggccgcgcct gagcatcatc
     3421 gccgcgatga agatcagcag gaacccgggc acctcgcgga tggcgatcag atagccattg
     3481 agctcgccgt ccatgcccag gctgtcgcgg aaaaagttcg cctggatcga atcgaacgag
     3541 ctcagggcca gggtcatgca gaacgtagtg atcgccagga cccggaagcc aaaatcgagt
     3601 tgttcgcgtg aaagagtttc tgtacgggaa tctatcgtcg ccgtgatcgc agccacccga
     3661 atgccgaccc tcccctggcc caatacctcg taccgggatt ctacgctaat cggtgacggt
     3721 tcccggcctg gtaatcaggc gaacgcggcg gtagaaggtg aagtaactcc agacccactg
     3781 ggtcatcacg acgaatcgat tccggaagcc gatcaggttg accaggtgga tcgtcagcca
     3841 gatgacccag gcaatgaacc catgcagccg gattccgtgg atgtcggcca ccgcccggtt
     3901 gcgcccgatc gtggccatat tgccgagatc cttgtaccgg aacggctcgg tgggctcggc
     3961 cgcgacacgg cgcacgatgt tcttcgccgc ggcacggccc tgctgcatcg ccacttgcgc
     4021 cacgccggga agctgcttgc catccttccc gttcagcgcc gccaggtcac cggccacgaa
     4081 gacattcgga tgtccgggaa ccgagagatc cggattgaca accacgcgac cggcccgatc
     4141 gagctcggtt ccgagcgtcc ggcccagtgg cgaggcctgg accccggccg cccagagaat
     4201 cgtcgccgcc tcgatccgtt caccgctctt gagggtcacg ccgtgggcat cgacgccggt
     4261 caccagggca ttgagtcgca catcgacgcc gagtcgctcg aggtcggttt gcgcgtgctt
     4321 cgaaagcgat tccggataag cgaggagcaa tcgcgggccg gcgtcgatca acaggatctt
     4381 cgcctggcga gggtcaatca cgtcgaagtc gcgggagatg ctgtggcggg cgatctcggc
     4441 aatcgccccc gccagctcga ctcccgtcgg gccgccgccg acgatcgcga aggtcatctc
     4501 gcggcggcgc tcgtcgtcgc tgtgggcacg ctcggctgct tcgaatccga gcaggatctg
     4561 attccgcatc cggaccgcgt catcgagcgt cttcaggccg ccggcgtact tctcccactc
     4621 gtcatggccg aaatagctgt gcgtcgagcc ggccccgatg atcagatagt cgaaaccgag
     4681 ctcgtcgtcc cccatctgga tgacgctgcg cgccagatcg atcccggtga cttcatccag
     4741 gatcacccgc agatacttct gactgcgcag gattccgcgg atcggaccgg cgatatcgac
     4801 cggcgagagc gatgcggtgg cgacctgata gaggagcggc tgaaaggtgt ggtggttgtg
     4861 ctgatcgacg atcgtgaccg cgacatcggc gtcccggagc tgtttggccg cgttcagccc
     4921 cgcgaacccg ccgccgatga tgacgacatg cggcttgcct gcctccgcca tggctcctcc
     4981 cttcccggtc aatacggaat cagctctgtc tccacctcgc ccatagagcc cagctcgaca
     5041 ttgccatcga caaacgcgat cacggccgcc aggatgtcgc tgcaatgcgc gccgctgttg
     5101 ctgaccaccg ccacgccgat cgtcgccacc cggtggacat cgagatcggc gacctcggct
     5161 gcggcaacgt tgaagcggtt gtggatgcgc tggatcagcg atttgaccac acgccgcttg
     5221 tccttcagcg agtcggccgc ctcgagataa agcgtgatct gggcgacgcc gatcgtcata
     5281 gcaccggcgc cgggcggatg gtgatccgga cgtgctcgcc atgccgctcc cagtggtacc
     5341 agacaccgca ggccagcgcg atcggagcga tcagatcgta gaggagatcg ccctcgacgt
     5401 tgtcgagtcc cagcccctcg actcgagccg ggtccgggaa atccgcgatc attgccgtgg
     5461 cctcgtcctc gctgaactcg gcctggacgg cctcgccgcc gaacgcagtc agattgacga
     5521 tggttgcgtc gaggaaatcc tcgggactcc aggcttcatc ggtgtcccag ccgcgcgcga
     5581 aaatttcgcc gagcgacttg gcccattctg tggccgggat gccctgaatc cgcaggaatg
     5641 agatcgtgcc cagcgccatg gccagcgtat tgcttcggag caattcgagc tcatcctcga
     5701 catcgaattc ggcgccgata tcctcctgaa aatcgtctgc caccggatgc tcctcctcac
     5761 tcaactgccc acactctagc agccgcgtcc gtttccgggg cgagattgcg cggagccgcc
     5821 cgaatcggta ggctgcccgg aaccgggaca ttgacaagcg agcgtgaaag gaccgccgga
     5881 tgccagccaa tacctacgat gtcgtgattg tcggcggcgg acacaatggg ctggttgctg
     5941 cctgttacct ggccaaagcc ggccattcgg tctgcgtact cgagcggtac cacgaggtcg
     6001 gcggcgcggc cttcacgcag gagatccacc ccggctacct gctctcggag ggctcgtacg
     6061 tcctgtcgct gatgccacgc aaaatcatcg atgagctcgg cgtgaccgac gacatccagc
     6121 tcgtcaaacg aaatccgcgc ttcttcatgc ccttccccga cgggcaatcg ctgactgcct
     6181 gggaggacga cgcgaagttc ctcgacgaga tccgcaagtt ctcgaagaag gatgccgaga
     6241 actacccgcg ctatgacgcc ttcgtcgaaa aggcatccga ggtcatggac cagttcatcc
     6301 tgcgccagcc gccgaccttc gccgagtttg cgagccagtt caagactacc gacgaggcgc
     6361 ggatcttcca gaagatgatc ctcggctcgg ctgccgaagt cgccgagtac tttttcgaga
     6421 gcgagcagct ccaggcaaat gcggtcgcgt tcggtctgat cggcaccttc cgcggtccgc
     6481 gcgacgccgg caccgcctat gtcaagctct accacgcgat ggggaatgtg accggccggc
     6541 gcgggcaatg ggcctatgtc aagggcgcca tgggcgcggt cagccaggca tacaagcgcg
     6601 tcgcgcttgc gaacggcgtc accatccgga ccagcgccga ggtggcgcag atctgcgtcg
     6661 agaacgggcg cgccaccggc gtcgtgacga cggacgggga ggacttccat tcctcggtcg
     6721 tgttctcgaa cgccgatccg caacggacct acctgaagct gaccgg
//
LOCUS       DATAOZ010000185         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6389893, whole genome shotgun sequence.
ACCESSION   DATAOZ010000185 DATAOZ010000000
VERSION     DATAOZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6389893"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VHR64_05005"
     CDS             <1..399
                     /locus_tag="VHR64_05005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3302218.1"
                     /translation="DPGFRIADTPLDMRILDRFTDILGPLVPALGDTPVAEHRGGLFT
                     MTPDARFLAGPIPSLRGLWTATGCNGSGFSNSAGIGRALAEWIVGGEPPFDLSSLDPG
                     RFGPGPFADEDLTAAGVWRYANYYTPQPVG"
     gene            complement(459..866)
                     /locus_tag="VHR64_05010"
     CDS             complement(459..866)
                     /locus_tag="VHR64_05010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX3302219.1"
                     /translation="MFTEKEMVYLQSQRLARLATVSPTGQCDADAVGFELDGEVFVIG
                     GRSLTESRKFKNVAAGNEQVSLIVDDIASLDPYEPRGIKMHGIASIVSRDGRFGPGEY
                     IEFRPRTSWSWGVEAPTFGDGNVRFHRSKHRQS"
     gene            996..2009
                     /locus_tag="VHR64_05015"
     CDS             996..2009
                     /locus_tag="VHR64_05015"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3302220.1"
                     /translation="MNDSVRSVDMAEERFLVTGALGCIGSWAVKRLVEEGVPVTTYDL
                     PGDPYRMRLIMDNDAINRVDFVQGDITDESRFMEVVREKNITHILHLAALQVPFVRAN
                     PILGARVNVVGTAIVFETARMLPDQIEGLVYASSIAVYGTPDLYPPGPLADDAPLAPV
                     TLYGVTKEANEGWASVYWQDYGLASVGLRPFFVYGPGRDQGVSSTPTKAMAAAAMGRP
                     YHISFGGTDTYQHADDVARAFIAAARTNPHGAPVYNLGGTLASVDDVVSAIEHVVPES
                     AGTITFNAEPLFTPNGVEGKAVEALLGPIAWRPLVEGVRDTIDVLRTAAAAGRLDVDR
                     AIA"
     gene            2017..>2508
                     /locus_tag="VHR64_05020"
     CDS             2017..>2508
                     /locus_tag="VHR64_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HEX3302221.1"
                     /translation="MPRLGVALRNLFGTSRFVIALAVAGAFLGSVLLLVVSTLTVIRL
                     AWRQFVEFDADNLTGKHLDHLGVSLIEITDMILLGMVLYIVSLGLYQLFIDPTLAVPG
                     WLRVNDIGDLKRDLINVTVVLLGVSFLGEVVDWDGTTDILSLGAAVALVTVALGLIMW
                     LTPR"
BASE COUNT          450 a          789 c          780 g          489 t
ORIGIN      
        1 gaccccgggt ttcgcatcgc cgataccccg ctcgacatgc gcatcctcga ccgcttcacc
       61 gacatcctgg gaccgctggt gccggccctg ggcgacaccc cggtcgccga gcaccgcggc
      121 gggctcttca ccatgacgcc cgatgcgcgc ttcctggcgg gaccgatccc cagtctgcgc
      181 ggtctctgga cggcaaccgg ctgcaacggc agcggcttct cgaactcagc cggcattggc
      241 cgggcgctgg cggagtggat cgtcggtggt gagccgccct tcgatctttc atctctcgac
      301 cccgggaggt tcggcccggg gccgtttgcc gacgaggatc ttacggccgc cggtgtctgg
      361 cgctacgcga actactacac cccacagcca gtcggttaag cgctatcgtc ggactgtcag
      421 gattcgcgca gcgacaaacc cgggcggtaa ccagcccctt agctttgccg gtgcttgctc
      481 ctgtgaaatc gaacgttgcc gtcgccaaag gtcggcgctt caacgcccca gctccaggag
      541 gtgcggggcc gaaactcgat gtactcaccc gggccaaagc gaccgtctcg cgagacgatc
      601 gaggcgattc cgtgcatctt gatgcctctc ggctcatagg gatcgagcga ggcaatgtca
      661 tcgacgatga gcgagacttg ctcattgccg gccgcgacgt tcttgaattt ccgcgactcc
      721 gtcaacgacc gcccgccgat gacgaagacc tcgccgtcga gctcgaagcc gaccgcatcg
      781 gcgtcgcact gacctgttgg cgagaccgtg gccaggcggg ccaggcgctg agactgcaga
      841 tagaccatct ctttctcggt aaacacggtc tccactccga acgcgatagc agatgaacac
      901 acaatgcctc aactacgggc attgtctagt attctgagtg gaaggcgcga aaccagagca
      961 accgacgaca ccgggaagcg cgtcagattg gcggcgtgaa tgacagcgtg cggagtgtag
     1021 acatggcgga ggaacgtttc ctggtcaccg gggcgttggg atgtatcggc tcgtgggcgg
     1081 tcaagcggtt ggtcgaagag ggcgttccgg tgacgaccta tgaccttcct ggtgacccct
     1141 atcggatgcg gctcatcatg gacaacgacg ccatcaaccg cgtcgatttc gtgcaaggtg
     1201 acatcaccga cgagtcgcgc ttcatggagg tcgtgcggga gaagaacatc acccacatcc
     1261 ttcacctggc ggcgttgcag gttcccttcg tacgagcgaa tccgattctg ggagcaaggg
     1321 tcaacgtcgt tggtacggcc atcgtcttcg agaccgcccg catgctgccc gatcagatcg
     1381 aaggactcgt ctacgcatcc tccatcgccg tctatggcac gccggacctc tatccaccgg
     1441 ggcccctggc cgacgacgct cctctggcgc cagtcacact ctacggcgtg acgaaagaag
     1501 cgaatgaagg ctgggcatcc gtctattggc aggactacgg gctcgccagt gttggcctgc
     1561 gtcccttctt tgtctatggg cccgggcgcg accagggcgt cagctcgacg ccaacgaagg
     1621 cgatggccgc cgcggccatg ggccgtccct accacatctc ctttggcgga accgatacct
     1681 atcagcacgc tgatgacgtc gccagggcct tcattgccgc ggcccgcacc aatccgcacg
     1741 gcgccccggt ctacaatctt ggcggaaccc tcgcgtcggt ggacgacgtg gtgagtgcca
     1801 tcgaacacgt ggtccccgaa agtgccggca cgatcacctt caatgccgag ccattgttca
     1861 cgcccaatgg cgtggaaggc aaagcggtcg aggcactgct cgggccaatc gcatggcggc
     1921 cgcttgttga aggggtgcga gatacgatcg acgtgttgcg aacagcagca gcggcaggac
     1981 gcctcgacgt cgatagagcg atagcgtaga cgagtgatgc cacgactcgg agtcgcgctg
     2041 cgcaaccttt tcggcacgag tcggttcgtc attgcgctgg ccgttgccgg ggcgttcctg
     2101 gggtcggtcc tgctgctggt ggtgtcaacg ctgacggtga ttcgtttggc gtggagacag
     2161 tttgtcgagt tcgacgccga caatctcact gggaagcact tggaccacct tggtgtcagc
     2221 ttgatcgaga tcacggacat gatcctgctg ggcatggtgc tctacatcgt gtccctgggg
     2281 ctctaccaac tcttcattga tcctacgctg gcggtgccag gctggctccg cgtcaacgac
     2341 atcggggatc tcaaacgaga cttgatcaac gtgaccgtcg tgctgcttgg ggtcagtttt
     2401 cttggtgagg tggttgactg ggacggaacg acagacatcc tctcccttgg cgccgcggtt
     2461 gccctggtga ccgttgccct cggcctcatc atgtggttga cccccagg
//
LOCUS       DATAOZ010000186        13442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3232948, whole genome shotgun sequence.
ACCESSION   DATAOZ010000186 DATAOZ010000000
VERSION     DATAOZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13442
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3232948"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1286)
                     /locus_tag="VHR64_05025"
     CDS             complement(138..1286)
                     /locus_tag="VHR64_05025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3302222.1"
                     /translation="MKPVLAHVITGLNTGGAEMMLANILAHHQQSHFRPVVYCLAEQG
                     PIADRIRALGVPVHSLDMRSGLPDPRAIRRLARALREEQAVVAQTWLYHADLIGGLAA
                     RMAGIPVVWGIHNSTLHGNASSRGLRMVIRVCRWLSPVVPSAIVCVAESAKAAHVAMG
                     YPARKMTVIPNGFDLTRFRPNPDARRRIRAELAIDPDAPVILIAGRFAPLKNHRMFVA
                     AAEIVANRYPETHFVMVGDGLGATNRELTEWVEQTGHPTRFHLLGRQSDMPELLAAAD
                     LIALTSNTEAFPLVIGEAMASGVPCVCTDVGDARSLIGDTGSIVPVGATAAFAEAIGE
                     LLAMPPAKRHALGCRARAKMEAEYDLGRIAARYDQLWQSLASGEKPCA"
     gene            complement(1283..2515)
                     /locus_tag="VHR64_05030"
     CDS             complement(1283..2515)
                     /locus_tag="VHR64_05030"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX3302223.1"
                     /translation="MINLGSAYRKFLVIFAVGIFFTNIADFSIKYGLIPLLWIGLFAV
                     VSAPAVLSGLAYSRIPVQPIIFWSAFYVAISILWYYHSPQDEFAFQEVQTRIVSVIFL
                     VLALIVFNGPDLQRLAQKWIAGAVLLATALNIYELFNPMTFSTIPGRSSGLYNNVNQS
                     GAALVLGLILSYQVIPDRFKMAYVGITALGVIPTFSRSAMIGWMLVVGYFFVRAGFRA
                     QIRRLSILAVVCVGVLFSPLWGDIQYSLQQRGVLTLNIVERVQFFTGGGRGDDSSNER
                     KAVATRAWELYGERPVTGWGTGASRRIEGFDVGTHNIYLAMLVDHGVIGFFIIPFLLL
                     SVLWGLNRKTIDLAAPWLIFMIMWGMFSHNVLEERYILLAVALMASMIAANRTAPDPA
                     EQSAMVPVQPLGDLVPLA"
     gene            complement(2512..3681)
                     /locus_tag="VHR64_05035"
     CDS             complement(2512..3681)
                     /locus_tag="VHR64_05035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3302224.1"
                     /translation="MKLLFLHRFKDVEGVEREFGGAERQLVDLVCGLQSRGHLVTLVT
                     FYPGGGMLADAAKAGVRIISLDKTGRWDIPGFLFRLVRILRSERPDIVHGYLGLANSL
                     LALTRPVHRAKVVWGIRASDLDLTRYHWVARVDAWLERRLSRLPDLIIANSHAGKRHA
                     TDLGYPAAKITVIPNGIDLDRFRRDETGRDRVRAEWGVRPEELLVGRIGRIDPQKDQP
                     TFLESAAKVAATRDDVRFVIVGNDRFGEQDEITPLVARLNLGDRLIWAGHRSDMAAVH
                     SAFDLCVSSSAFGEGTPNVLVEAMACGTPCVTTDVGDSAQAVGAHGIVVPRRDPAALA
                     DGILTAVDQPVDRTALRSHVADHFSMERLIAATEAALLELRAPTARANRAQAVQP"
     gene            complement(3678..4784)
                     /locus_tag="VHR64_05040"
     CDS             complement(3678..4784)
                     /locus_tag="VHR64_05040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3302225.1"
                     /translation="MTADRITLVIPTLTSGGAERVLTTMANHWARQGRSVTVLTLAAQ
                     EETPFYPLAPGVHRVGLGTIRPARTPIHAAINNAGRLSRLRSAIRASRPGVVISFLDQ
                     TNVLTLLATRGLRMPVVVSEHTDPGLAQRHPIWDRLRSLLYPRADRVVVLTKSSKAFF
                     APKIRAKTRIVPNPIQVDCPSAPIHKSSRPQIVAMGRFGPEKGFDRLIDAFSRIAADF
                     PDWDLVIWGDGALRPELTAQRDRLGLTDRILLPGRTTEPHAELRKTSIYALTSHREGF
                     PMALAEAMACGLPAVAFDLPSGPRDIIRAGIDGELVPNGDIPAFAEALASLMRDCARR
                     QQMAERAPDVLDRFGVERVMAIWDDLLGELTTTR"
     gene            complement(4781..6157)
                     /locus_tag="VHR64_05045"
     CDS             complement(4781..6157)
                     /locus_tag="VHR64_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX3302226.1"
                     /translation="MAVQSRALPNSGRLSRLGNHRQLLLNIAVVAFCSVGVKIAGMLR
                     DVVLASEFGTSDAADAFIAAWAIPQFLALIIGNAFAGVIIPLQAEAHGRGGEHQSRRF
                     LGEMLLISIVGLAAVTLALIPLRDSLLPLVTSNFGPAKLTQTRDLWMIMLPAIFLFAM
                     STIWSAMLNTGDRFGLAAMSPVVIPAATIGALWLHPEGGITSPTIGFVIGSLAQAVCL
                     IIGLHRHNLDILPKWHGGLDETRMALRQFVPYLANGVVFGGVGVVDQAMAATLGQGSL
                     AILSFGNKLVLPVLAIGSAALATVVYPRFSRLVAEREWIRLHQQVKSYLALVLVATIP
                     ATVVIVLLSTFIVRVLFEHGEFNPSDTRDVAELQMIFALMIPAYTLSQLLSRVLNAMR
                     ATRYLLIGSIVIFAFNIIADYLFKEWFGITGIAMATVGNYALALIFNFYLFRRLMSAR
                     LSEPAGTR"
     gene            complement(6448..7845)
                     /locus_tag="VHR64_05050"
     CDS             complement(6448..7845)
                     /locus_tag="VHR64_05050"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX3302227.1"
                     /translation="MSRIWTLVFLILAVSAGATSHAWAQADAAPLSVAAAGLVNPRGF
                     AFTSEGQMVVAEAGTGGDTAATEDVPAPTGPYTGGPTGAVVRIAASCPAPLAGNLASA
                     RGAGGESIGPSAVVIIGDKTYALVAGGGAAHGNPDQPAGIYDVTTGSPVLLADLSGWL
                     RANPVANPPENDFDPDGSWYSMATDSAGSALWVVESNSEQVLRVDLDGTITRIADLSA
                     ENQVPTAIAIGPDGAIYVGHLTSAPFTAGSATVIKIGADGKSEVVWSGLTMVTGLAID
                     RQGTLYASELSGGRDHPPFFVPGTGRIVRQTGADSLEEVATLLNLPTTIQFGPDSGLY
                     VSLPAVGADNGTGVILRVDTAAQLPLRAEDYDLTPPSCGGAEQGSTLIKLSDLGIDPT
                     SITVTAGTTVTWRNTGEFEHSTTSDPSSPVQWDSGSLIPGQEFSQVFDKPGTYAYFDG
                     VFPDHRGTIEVIAAE"
     gene            complement(7890..13409)
                     /locus_tag="VHR64_05055"
     CDS             complement(7890..13409)
                     /locus_tag="VHR64_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX3302228.1"
                     /translation="MPLQGKRWPLHVWVSYEELVQSGAPIVIARTDPDLPKTLARLMD
                     LHGAQSYLAIPGYTGGKVTSIIQIMSRAPILRESAETRRIWMTIATQIQHGIEAVTAL
                     ETSQRRERHEAMRRRINESLQVAGNLQDSYETIAGIFHEIPDVDGITIMSWHPESQHY
                     VVEAEWTVPPIPCSPVGTIHEKDDYLLLKEIDLETSVRMRWIGQHGLCDEQRARMASY
                     HIGAAIAMPLRVEDQFVGALMLYSLRHRAFPADTVEWAQELAGTMALVVRTIELRRRD
                     EQLREEQSLKLRMLQAATSKAEPIEALMAIAEAGLGWAGAESINIGFINPDRWELTLA
                     ADCTVPDWPGVEEPGTVLKIESGSLQERSFQTLQPTMFRSDQPELGKWDLEDIAMYGA
                     HAWIAAPLRVGGQAIGLLQVLSRDPNAFDQRRLRLWDEIAQQVSLVTSNLQVSFDNRR
                     LSHQQQLVSLVSQAGVTADSRDDAYRRLAEIGLGLAGVESSTIFLWHEDDGLLEIAHE
                     AVTNGFTPHYRSGERIIPPGYPQHDGTFRIERTGHYYLDDESLPVLERAMLDKLRVGR
                     TAIYPLRDESRVYGVLSLKARDSGPFPTETVRLAEMIALTATAVVRNIRSREEEQRTE
                     REQHALLRLSRAATSCASVEELMGVVADTIRELCAASSAQVFTFVGGSRTLLNASSTD
                     ETWRDTSPIGTTWPIGAWPIEDALRTTSEPIVVLDRTDPRLSPAMLADLVRDGIHSLA
                     CMPLAIGDGIQGYVGVFSARPRAFTSALVRSTATVVNQAALAIDHTRTRLEEMRTAAQ
                     QELLLRVSQAAGSSLELMTVLSQIAEASLGVANSESCVIELYDPETDEMVLHALSRVH
                     DWVIVEDMSFKRRPFGSWSIDEQVRADGWIVIGSIADARVDGLMHADWERYQTSSAIS
                     HALISGGVFIGMYHCFARHENAYAAQDRWVTSEIAAHVVAAIQNARMLEHEHQVANER
                     ARLLQITEAATRTLNLSQVLQEIASATLGLAGAESCHIELVDHDQNEFVTEAYAAVPG
                     WQAMTWAQFGARAKIGAWSIDDDTMTSNAPVSIGSIEDPRVYGRLREDMEAFGTQSIL
                     MIPIWLDDRCVGILNLYSRKRHAFSNAQQTIAHTLATHAANAIQNARTHERERARRQH
                     QEALVELTTAATSSLDVNEAMHRVCIALCDVVDAESASVGLLRPELAAFEIVADVTAP
                     DWDFADEPGDLDPIDNYPSYQRVLTSQGAFLVSRDHGTPEQMQFIEHHEVGLETYVLA
                     PLRLNDVCLGYVGVYDRRPGQFGQEVITLIDSASSLIALSLSNGLTAREQQRRATDRA
                     AVVNVGRAAISDADVSQKLQQIGEACLDIHRVDGVSIYQWDPESSELVCMADVGRPGW
                     PYRLEVGYRFPVPEDSAFRPFLEARDASSFTLLDQALHPQLRKDMEQSRGKASAIFPL
                     RADDQLLGIAAFYSLEPHPFDDETIRVGREIATQTALAIRSSRLLETTRRYANEQSAL
                     LAVNRAVMDATLTSIDAVLEKISSETMNLIGAECCEIEGLAPDLNCTVMLAQVLIDDW
                     RGLTSGPGLEMPLSDWPMTRYVLEQQRPVVLDSEENDLTEHERAGLFALDTKSGLIAP
                     MVIGGRSVGTISFFSRERYAFTPDKVRLAVEFGSLAALAIDRARTHMALAEQATIDGL
                     TSVLNHRAFLERLDHQMAIADRTEDPVSLLMIDMNDFKQINDRYGHLAGDGVLRETAR
                     FFTQSLRASDLVARYGGDEFAAILPGTDVADAIHLRERLMDLAVTYYIDMADGSQVVP
                     SFSIGVAAYPTQANDRQSLIDAADRSMYESKDGTAGSPARNLRLPASEAVVRLTG"
BASE COUNT         2379 a         4100 c         4743 g         2220 t
ORIGIN      
        1 cgccaattgc tgggtcagtg gaccgccgat accggtgact acactcacct cgtggccgcg
       61 atcccgcgtg ccgatggcga ggtcacgcac catcatctgg gcgcctccga tgtcgtcgcc
      121 gcgcgtaatg acgtacatca cgcgcatggt ttttctcccg acgccagcga ttgccagagt
      181 tgatcgtacc tggcggcgat tcggcccaga tcgtattcgg cctccatctt cgcccgggca
      241 cggcaaccga gcgcgtggcg tttcgccggc ggcatggcaa gcagctcccc gatggcttcc
      301 gcgaaggcgg cggtcgcgcc gaccggtacg atgctgccgg tatcgccaat caatgagcgc
      361 gcgtcgccga catcggtgca gacgcagggg acaccgctcg ccatcgcctc acctatcacg
      421 aggggaaagg cctcggtatt cgaggtcagg gcgatcaggt cagcagcggc tagcaactcc
      481 ggcatatcgg attgacgccc caacagatgg aatcgggttg gatggccagt ctgttcaacc
      541 cactcggtca gctcgcgatt cgttgcgccc aggccatcgc cgaccatcac gaagtgggtc
      601 tccgggtatc gattggcgac gatctcggct gccgcgacga acatccggtg attcttgagt
      661 ggcgcgaacc gcccggcgat caggatgact ggcgcgtccg gatcgatggc gagctcggcc
      721 cggatgcggc ggcgggcatc ggggttcggc cggaacctgg tcaggtcgaa gccattcggg
      781 atgaccgtca tcttccgggc ggggtatccc atcgcgacgt gggccgcctt cgccgactcg
      841 gcaacgcaga cgatcgcact cggcacgacc ggcgagagcc agcggcaaac ccggattacc
      901 atgcgcaatc cccggctgga cgcgtttccg tgcaaggtgc tgttgtggat accccagacg
      961 accgggatgc ctgccatccg ggcagcgagt ccgccgatca ggtcggcgtg atagagccaa
     1021 gtttgggcga cgaccgcttg ctcctcccgg agggcgcgcg ccaggcggcg aatcgcgcgc
     1081 gggtcgggaa ggccactccg catgtcgagg ctgtggacgg gaactccgag cgcgcgaatc
     1141 cggtccgcga tcgggccctg ctcggccagg cagtagacga ctggccgaaa atgcgattgc
     1201 tggtgatggg cgaggatgtt ggcgagcatc atctcggcgc cgcccgtatt gaggccggtg
     1261 atgacgtgcg cgagaaccgg cttcatgcca gcgggaccag gtcgccgagt ggctgaacgg
     1321 gaaccatcgc cgactgctcc gccggatcgg gcgcggtccg gttagcggca atcatggagg
     1381 ccatcagcgc gaccgcgagc aggatgtagc gctcctcgag cacgttgtgg ctgaacatgc
     1441 cccacatgat catgaagatc aaccatggcg cggccaggtc gatcgtcttg cggttcaacc
     1501 cccaaagcac gctcagaagc aagaagggga tgataaaaaa gccgatcacg ccatggtcga
     1561 ccaacatggc gaggtaaatg ttgtgcgtgc caacatcgaa cccctcgata cggcgactcg
     1621 ctccggtgcc ccagccagtg actggtcgct ccccatagag ctcccaggct cgggtggcga
     1681 ctgccttgcg ctcgttcgac gagtcgtcgc cgcgtcctcc gcccgtgaag aactggacgc
     1741 gctcgacgat gttcagggtc aggacaccgc gctgctggag tgagtactgg atgtcgcccc
     1801 agagcggcga aaagagcacg ccgacgcaga cgacggccag gatcgaaagc cgccggatct
     1861 gggcgcgaaa tccggcccgg acgaaaaagt aaccgaccac gagcatccag ccgatcatcg
     1921 ccgagcgcga gaaggtgggg atgacgccga gggcggtaat cccgacgtag gccatcttga
     1981 agcggtccgg gatgacctgg tagctgagga tcaggccgag cacgagcgca gcgccgctct
     2041 gattgacatt gttgtagagg ccggaggagc ggccggggat cgtgctgaaa gtcatcgggt
     2101 tgaagagctc gtagatgttg agcgcggtcg cgagcaggac cgcgccggcg atccacttct
     2161 gggccagacg ttgcaggtcg gggccgttga acacgatcag ggcgaggacc aggaagatga
     2221 ccgagacgat ccgggtctgg acctcctgga aggcgaactc atcctgtgga gagtggtagt
     2281 accagagaat gctgatcgcg acgtagaagg ccgaccagaa gatgatcggc tggacgggga
     2341 tacgtgagta cgccagaccg ctgaggaccg ctggcgcgct gaccacggcg aagaggccga
     2401 tccagagcaa agggatcagg ccgtacttga tcgagaagtc ggcgatgttg gtgaaaaaga
     2461 tcccgaccgc gaagataacc aggaacttcc ggtaggcact gccgaggttg atcatggttg
     2521 caccgcctgc gcgcggttag ccctggcggt gggtgcacgc aattccagca gggccgcttc
     2581 ggtcgcggcg ataaggcgct ccatcgagaa gtgatccgcg acgtgggagc gcaacgcggt
     2641 gcggtcgacg ggctggtcga ccgcggtcag gattccatcg gccaaggctg ccgggtctcg
     2701 ccggggcacc acgatgccgt gggctcccac tgcctgagcc gagtcgccga cgtcggtcgt
     2761 gacgcacggt gtgccgcagg ccatcgcctc gaccagcacg ttgggcgtcc cttctccgaa
     2821 tgccgaagag gagacgcaga ggtcgaatgc gctgtggacg gcggccatgt cggagcggtg
     2881 gccggcccag atcaggcgat cgcccaggtt aagccgggca acgagtggcg tgatctcatc
     2941 ctgttcgccg aagcggtcgt tgccgacgat cacgaagcgg acgtcgtcgc gggtcgcggc
     3001 gactttggcg gcggattcga ggaaggtcgg ctggtccttc tgcgggtcaa tccggccgat
     3061 tctgccgacg agcagctctt cgggtcggac gccccactcg gcgcggacgc ggtcccgacc
     3121 cgtttcgtcg cgccggaagc ggtcgaggtc gatgccgttc gggatcaccg ttatcttcgc
     3181 ggcggggtag ccgaggtcgg tggcgtggcg cttgccggcg tggctgttgg caatgatcag
     3241 gtccggcagg cggctgaggc ggcgctccag ccaggcatcg accctggcca cccagtggta
     3301 gcgggtcagg tccagatcgg aggcgcggat cccccagacg accttcgcgc ggtggacagg
     3361 tctggtcagg gcgagcaagg agttggcgag gccgagatat ccatggacga tgtcgggccg
     3421 ctcgctccgg aggatgcgga cgaggcggaa caggaagccg gggatatccc agcgcccggt
     3481 cttgtcgagc gagatgatgc ggacaccggc cttggcagcg tcggccaaca tgccgccacc
     3541 cgggtagaag gtgaccaggg tgaccaggtg accacgggat tgcaggccac agacgagatc
     3601 gaccagctgg cgctcggcgc cgccgaactc gcgctcgacg ccctcgacat ccttgaatcg
     3661 gtgcaggaag agcagcttca tcgggttgtc gtcagctctc ccagcaggtc gtcccagatc
     3721 gccatgacgc gctcgacacc gaagcgatcg agcacgtccg gagcgcgctc tgccatctgc
     3781 tggcgacgcg cgcagtcccg catcagcgat gctagggctt cggcgaaggc ggggatatca
     3841 ccatttggga caagttcgcc gtcgatgccg gcgcggatga tgtcgcgcgg accgctgggc
     3901 agatcgaagg cgacggccgg cagcccgcag gccatcgctt cggccagggc catcgggaag
     3961 ccctcgcggt gcgaagtcag ggcgtagatc gatgtcttgc ggagttccgc gtgcggctcc
     4021 gtcgtgcggc cgggcaagag gatgcggtcg gtgaggccaa ggcggtcgcg ctgggccgtc
     4081 aattcgggtc gaagggcgcc gtcgccccag atgacgagat cccagtccgg gaagtcggcg
     4141 gcgatccggc tgaaggcgtc gatcaaacgg tcgaagccct tctcgggacc gaagcggccc
     4201 atggcgacga tctgcggccg ggaagacttg tggatcggcg ctgacgggca gtcgacctgg
     4261 atcgggttcg gaacaatccg cgttttggcc cggatttttg gcgcgaagaa cgccttgctc
     4321 gacttggtga gcacgacgac gcgatccgcc ctcggataga gcagcgagcg gaggcggtcc
     4381 cagatcgggt ggcgctgggc caggccggga tcggtgtgct cgctgacgac gaccggcatg
     4441 cgcaaacccc gggtcgcgag cagggtgagc acgttggttt ggtcgaggaa gctgatcacg
     4501 acgcccggcc ggctggctcg gattgcgctc ctcagtcggg acaagcgccc ggcgttgttg
     4561 atcgcggcgt ggataggcgt gcgagcgggt cggatggtcc cgaggccgac gcggtggacg
     4621 ccgggagcga gcgggtagaa gggcgtctct tcctgcgcgg cgagggtcag gacggtcact
     4681 gatcgcccct gcctagccca atggttggcc atggtggtca gcacacgctc ggccccgccg
     4741 ctggtcaggg tcgggatgac aagggtgatg cggtcggccg tcatcgggtt ccggccggct
     4801 cactgagacg ggcggacatc agtcgacgaa agaggtagaa gttgaagatg agggcgagcg
     4861 cgtaattgcc gaccgtggcc atggcgatgc cggtgatgcc gaaccactcc ttgaagaggt
     4921 aatcggcaat gatgttgaag gcgaagatga cgatcgagcc gatcagcagg taccgggtgg
     4981 cgcgcatcgc gttcaggacg cgcgatagga gctgcgagag ggtgtaggcg gggatcatca
     5041 gcgcgaagat catctggagc tcggcgacat cccgggtgtc ggatggattg aactcgccgt
     5101 gctcgaacag cacgcggacg atgaacgtag agagcagcac gatcacgacc gtggccggga
     5161 ttgtcgcgac cagcaccagt gccagatagc ttttgacctg ctgatgcagc cggatccact
     5221 cccgctcggc caccagccgg gagaatcgcg ggtagaccac cgtcgcgagc gcggccgagc
     5281 cgatcgcgag gaccggcagg accagcttgt tgccgaagct gaggatggcg aggctgcctt
     5341 gtccgagtgt agcggccatg gcctgatcga ccacaccgac gccgccaaag acaacgccgt
     5401 tggcgaggta gggcacgaac tggcggaggg ccatccgggt ctcgtcaagg ccgccatgcc
     5461 atttgggcag gatgtcgagg ttgtggcggt ggagaccgat gatcaggcag acggcctggg
     5521 ccaggctgcc gatcacgaag ccgatggtcg gggaggtgat tccgccctcg ggatgaagcc
     5581 agagcgcgcc aatggtcgcc gcggggatca ccaccggcga catggcggcg aggccgaagc
     5641 gatcaccggt attgagcatg gccgaccaga tcgtcgacat cgcgaacagg aagatggccg
     5701 ggagcatgat catccagagg tcgcgggtct gggtcagctt ggcgggaccg aagttggagg
     5761 tgacgagggg caggagtgag tcgcggagtg ggatcagggc cagcgtgacc gcggccaggc
     5821 cgacgatgct gatcagcagc atctcgccca ggaagcggcg cgattggtgc tcgccgccgc
     5881 ggccgtgtgc ctcagcctgg agcgggatga tcacgccggc gaaggcgttg ccgatgatca
     5941 gggccaggaa ctgcggaatg gcccaggcgg cgatgaaggc atcggcggcg tcagacgtcc
     6001 cgaactcgga cgcgaggacg acatcgcgca gcatgccggc gatcttgacg ccgaccgagc
     6061 agaaggcgac gacggcgata ttgagcagga gctgccggtg attccccagg cggctgagcc
     6121 ggccggagtt cggcagcgcg cgactctgga cagccatgac tggaatcact caacgcgcat
     6181 cacggtccgt ctggtccgtg cgtcaggctc cagcatgccc cagaagtcac acgccgggat
     6241 cgggatcggg ccacccggta gtactaatgc ccgggtgacc aacctcccga gccgtgatcg
     6301 tccaacaaca gacgtagctc cggccccccg tggccggcat ggcgtcaacg ggacgcccgg
     6361 tcgacgccat gcgcctgctc catccgcatc atcgacaacc gggcggccca gaggcgggtc
     6421 gcgctacgtc acggaacgga cgccaggtta ttcggcggcg atgacttcga tcgtgccgcg
     6481 gtggtcgggg aagacgccgt cgaagtaggc gtaggttccg ggcttgtcga agacctggga
     6541 gaattcctgg cccggaatga gcgagccgga gtcccactgg accgggctgg atgggtccga
     6601 cgtcgtcgag tgctcgaact cgccggtgtt gcgccaggtc accgtcgtcc cggccgtgac
     6661 cgtgatcgag gtggggtcaa tgccgaggtc ggagagcttg atcagcgtcg agccttgctc
     6721 ggcgccccca catgatggag gcgtcaggtc gtaatcctcg gcccgcaatg gcagctgggc
     6781 ggcagtgtcg acccggagga tcacgcccgt gccgttatcg gcgccgacgg ccggcaggga
     6841 gacgtacagc ccgctgtccg gaccgaactg gatcgtggtc gggagattga gcagcgtggc
     6901 gacctcttcc aggctgtccg cgccggtctg gcgaacgatg cgcccagtac cgggcacgaa
     6961 gaagggcgga tgatccctcc cgccggacag ctcgctggcg tagagcgtgc cctggcgatc
     7021 gatggcgaga ccagtgacca tcgtcaggcc ggaccagacg acctcgctct tgccgtcggc
     7081 gcctatcttg atcacggtcg cggaaccggc ggtgaagggc gccgaggtga gatggccgac
     7141 gtagatggcg ccgtccggtc cgatcgcgat cgcggtcggg acctggtttt cggcggagag
     7201 atcggcgatc cgggtgatcg tgccgtcgag gtcgacgcgc agaacttgct cgctgttact
     7261 ctcgacgacc cagagcgcgg agccggcgga gtcggtcgcc atgctgtacc acgagccgtc
     7321 ggggtcgaaa tcgttttcgg gcgggttggc aaccgggttg gcgcggagcc atccgctgag
     7381 atcggcgagg aggaccgggc tcccggtggt gacgtcgtag atgccggcgg gctgatccgg
     7441 gttgccgtgg gcagcgccac cgccggcgac gagcgcgtag gtcttgtccc cgatgatgac
     7501 aacggcgctt ggcccgatcg actccccacc cgccccgcgc gcggaggcga gattaccggc
     7561 cagtggagcc gggcaactgg cggcaatgcg gacgacggcg ccggtcgggc cgccggtgta
     7621 gggaccggtc ggcgcgggca catcttcggt cgccgcggtg tcgccaccgg tgccggcttc
     7681 ggctacgacc atctggcctt cagaagtgaa tgcgaacccg cgcgggttga ccagtccggc
     7741 ggcggccacg gagaggggcg cggcatctgc ctgggcccag gcgtgtgatg tggcgcccgc
     7801 cgacacggcc aggatcagga agacgagagt ccagattcgc gacatgggta ctcctgtagc
     7861 gatcaggctg cccgatcgcg gcaggtcccc tatccggtca ggcggacaac tgcctcgctg
     7921 gcgggcaatc tgagatttct ggccgggctg ccggccgtac cgtccttgga ttcgtacatc
     7981 gaccggtcgg ccgcatcaat cagcgattgc cggtcgtttg cctgggtagg atacgcggcg
     8041 acgccgatcg agaacgacgg cacgacctgg ctgccgtcgg ccatgtcgat gtaataggtc
     8101 accgccagat ccatgagacg ctcccggagg tggatcgcgt cggccacgtc ggtgcccggc
     8161 aaaatggctg caaactcatc gcctccgtag cgagcgacca ggtcggatgc gcgcagcgat
     8221 tgcgtgaaga agcgggcggt ctcgcggagc actccgtccc cggccaggtg accatagcga
     8281 tcgttgattt gcttgaaatc attcatgtcg atcatgagga gggaaaccgg atcttcggtg
     8341 cggtcggcga tcgccatctg atggtcgagc cgctcgagga aagcgcggtg gttgaggacg
     8401 ctggtcaggc cgtcgatcgt cgcctgctcg gcaagggcca tgtgggtgcg ggcgcggtcg
     8461 attgccagtg ccgcgaggct gccgaactcg acggcaaggc ggactttgtc cggcgtgaag
     8521 gcataccgct cgcgcgagaa gaagctgatc gtgccgaccg atcggccgcc aatgaccatc
     8581 ggcgcgatca gcccggattt ggtatcgagg gcaaatagtc cggcccgctc atgctctgtg
     8641 aggtcgttct cttcactgtc caggacaacc ggccgctgct gttcgaggac ataccgagtc
     8701 atcggccagt cgctgagcgg catctccagg ccaggtcccg aggtgagtcc acgccagtcg
     8761 tcgatgagca cctgggcaag catgacggtg caattgagat cgggcgcgag tccttcgatc
     8821 tcgcaacatt cggcgccgat caggttcatc gtctcggacg agatcttctc cagcacggca
     8881 tcgatcgagg ttagcgtcgc atccatgacc gcgcggttga ccgccagcag ggccgactgc
     8941 tcgtttgcgt agcggcgggt ggtttcgagc aggcgagagg accgaatcgc caaggcggtt
     9001 tgggtggcga tctcccggcc gaccctgatc gtctcatcgt cgaacgggtg cggttccagg
     9061 gagtagaacg cggcaattcc aagcagctgg tcgtcggcgc ggagcgggaa gatcgcggag
     9121 gccttgccgc gcgattgctc catgtccttg cgaagttggg ggtggagggc ctggtcgagg
     9181 agcgtgaacg acgacgcgtc ccgcgcctcc aggaatggcc ggaaggcgct gtcctccgga
     9241 accgggaacc ggtagccgac ctccagccga tacggccagc ccgggcgccc gacatcggcc
     9301 atgcagacca gctcgctcga ttcgggatcc cactggtaga tcgagacgcc atcgacgcgg
     9361 tggatatcga gacaggcctc accgatctgc tgaagctttt gggagacatc ggcatccgag
     9421 attgccgccc ggccgacgtt gaccacggcc gcgcggtcgg tcgcccgccg ctgttgctcg
     9481 cgagcggtca gcccgttcga gagggagagc gcgatcagcg agctggcgct gtcgataagg
     9541 gtgatgacct cctggccgaa ttgcccaggg cggcggtcgt agacaccgac atacccgagg
     9601 cagacgtcgt tgaggcgcag cggcgcgagg acatacgttt ccaggcccac ctcgtggtgc
     9661 tcgatgaact gcatctgctc cggggtgcca tgatcgcggc tgaccaggaa cgcgccctgg
     9721 ctggtcagca cacgctggta cgaggggtag ttgtcgattg gatcgaggtc gcccggctca
     9781 tcggcaaagt cccagtccgg ggcggtgaca tcggccacga tctcaaatgc cgccaattcc
     9841 gggcggagaa gcccgacgct ggccgactcc gcatcgacca catcgcaaag cgcgatgcag
     9901 acccggtgca tggcctcatt gacatccagg ctcgaggtcg cggctgtggt cagctcaacg
     9961 agcgcttcct ggtgctgccg ccgcgcccgc tcgcgctcat gcgtgcgggc gttctggatc
    10021 gcgttggccg cgtgggtcgc cagcgtgtgc gcgatcgtct gctgggcatt ggaaaaggcg
    10081 tggcgtttgc gggaatagag gttgaggatg ccgacacagc ggtcgtcgag ccagatcggg
    10141 atcatgagga tactctgggt cccgaacgcc tccatgtcct cgcgaaggcg accgtatacc
    10201 cgcgggtcct cgatcgagcc gatcgaaacg ggcgcattgc tggtcatcgt gtcgtcatcg
    10261 atggaccagg cgccgatctt cgcccgagcc ccgaactgtg cccaggtcat agcctgccaa
    10321 ccgggcaccg cggcatacgc ctcggtgacg aattcattct gatcatgatc gaccagctcg
    10381 atatggcagc tctcggctcc ggccagaccg agggtggccg aggcgatctc ctggaggacc
    10441 tggctgaggt tgagggtgcg ggtggcagcc tcggtgatct ggagcaggcg ggcccgctcg
    10501 ttggcaacct gatgctcgtg ctcgagcatt cgcgcgttct ggatcgccgc gacgacatgg
    10561 gccgcgatct cgctcgtcac ccatcgatct tgcgctgcgt aggcattttc gtggcgggcg
    10621 aagcagtggt acatgccgat gaaaacgcca ccgctgatca gggcgtgcga gatggcgctg
    10681 ctcgtctggt accgctccca gtcggcgtgc atcaacccgt cgacgcgggc atcggcgatc
    10741 gagccgatca cgatccagcc gtccgcgcgc acctgttcgt cgatcgacca cgagccgaac
    10801 ggccggcgct tgaagctcat gtcctcgacg atgacccagt cgtgcacgcg ggagagagcg
    10861 tggagcacca tctcgtcggt ctcggggtcg tagagctcga taacgcagct ttccgaattg
    10921 gcaacgccca ggctcgcttc agcgatctgc gagaggacgg tcatcaactc gaggctgctg
    10981 ccggcggcct ggctgacccg gaggagcagc tcctgctgcg cggccgtgcg catctcctcg
    11041 agccgggtgc gggtatggtc gatagcaagt gcggcctgat tgacgacggt tgcggtcgag
    11101 cggaccaagg ccgaggtgaa tgcgcggggt cgcgcggaga aaacgccgac gtacccctga
    11161 atgccatcgc cgatcgcaag gggcatgcag gcaagactgt ggatcccatc gcgaaccaga
    11221 tccgcaagca tcgccgggga gaggcggggg tcggtgcggt cgaggacaac aatcggctcc
    11281 gaggtggtgc gcagtgcgtc ttcgatcggc cacgcgccga tcggccaggt cgtaccaatg
    11341 ggagaggtgt cgcgccaggt ctcgtcggtg ctgctggcgt tgagcagcgt ccgcgacccc
    11401 ccgacaaagg tgaaaacctg ggcgctgctc gccgcgcaaa gctcgcggat agtgtcggcc
    11461 acgacgccca tcagctcctc gaccgatgcg caactggtgg cagcccggga gaggcgcagc
    11521 agcgcgtgtt gttcgcgctc cgtgcgctgc tcctcctcgc gagagcggat gttgcggacg
    11581 acggcagtgg cggtcagggc gatcatttcg gccagccgga ctgtctccgt cgggaaggga
    11641 ccactatcgc gcgccttgag ggagaggacg ccgtagacgc gactctcgtc gcggagcgga
    11701 tagatggcag tgcggccgac ccggagcttg tcaagcatgg cccgctcaag cacggggagg
    11761 gattcgtcgt ccaggtaata gtggccagtc cgctctatcc ggaatgtgcc atcgtgctgg
    11821 ggatatcccg gcgggatgat gcgctcgccg gatcggtagt gtggagtaaa tccgttcgta
    11881 accgcctcgt gggcgatttc cagcaagccg tcgtcttcat gccaaaggaa gatcgtgctc
    11941 gactccaccc cggcgagccc caacccgatt tcagcgagcc ggcggtacgc gtcgtcccgg
    12001 gagtcggccg tgactcccgc ctggctgaca agggagacta gctgctgctg gtgcgacagg
    12061 cgacgattgt cgaatgagac ctgcaggttg gaggtcacca gggagacctg ctgggctatc
    12121 tcgtcccaca atcgcaggcg gcgctgatcg aacgcgttcg gatcgcgcga aagcacctga
    12181 agcaggccga tcgcctggcc accgacgcgg agcggagcgg cgatccaggc atgcgccccg
    12241 tacatggcga tgtcctcaag atcccactta ccaagctccg gctggtcgct gcggaacatg
    12301 gttggctgaa gggtctggaa gctccgctcc tgcaacgagc ccgactcgat tttgaggaca
    12361 gtgcccggct cttcgacgcc gggccaatca ggaacggtgc agtcggcggc aagggtcagc
    12421 tcccatcggt cagggttaat gaaaccgata ttgatcgact cggcgccggc ccaaccgaga
    12481 ccggcttcgg cgatcgccat cagggcctcg atcggttcgg cctttgaggt cgcggcctgg
    12541 agcatacgga gcttgagcga ttgctcctcg cgcagttgct cgtcgcggcg gcgaagctcg
    12601 atagtgcgca cgaccagggc catggttccg gccagctcct gggcccactc gaccgtatcc
    12661 gccgggaacg cgcgatggcg cagcgagtag agcatcagcg cgccgacgaa ttgatcctcg
    12721 acccggagcg gcatcgcgat cgcggcgccg atgtggtagc tggccatgcg cgcccgctgc
    12781 tcgtcacaaa gtccgtgctg cccgatccag cgcatgcgca ccgacgtctc gaggtcgatt
    12841 tccttgagca ggaggtagtc gtccttctcg tggatcgtgc cgaccggcga gcaggggatc
    12901 ggcgggacag tccactcggc ctcaacgacg tagtgctggc tctccgggtg ccagctcatg
    12961 atcgtgatgc cgtcgacgtc tggaatctca tggaagatgc ccgcgatcgt ctcgtagcta
    13021 tcctggaggt tgcccgcgac ctgcagcgat tcgttgattc gccgccgcat cgcttcgtgc
    13081 cgctcgcggc gctggctggt ttcgagagcg gtaacagcct cgatcccgtg ctggatctgc
    13141 gtggcgatcg tcatccagat tcggcgcgtc tcggccgatt cgcggaggat gggcgcgcgg
    13201 gacatgatct ggatgatcga cgtcaccttg ccgccggtgt aaccggggat ggccagatag
    13261 ctctgggcgc cgtgcaagtc catgagccgg gccaacgttt tcggaagatc gggatcggtc
    13321 cgggcgatga cgatcggcgc cccgctctgg acaagctctt catagctgac ccagacatgc
    13381 agcggccacc gcttgccctg aagcggcacc tcccgcgtgg caagactgcg ggcgtcggcg
    13441 gc
//
LOCUS       DATAOZ010000187         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6654771, whole genome shotgun sequence.
ACCESSION   DATAOZ010000187 DATAOZ010000000
VERSION     DATAOZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6654771"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VHR64_05060"
     CDS             <1..570
                     /locus_tag="VHR64_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX3302229.1"
                     /translation="LAIAAGRAVVEIRPFLPRSVAVISTEGLEDAVRERFRTAVNKKI
                     AWYGSSVSGFTDLPDDSDQVAQAIEAAIGDGAGLILTGGGNSLDPLDPTLVALEAMGA
                     EMIKFGAPAHPGSMFWLARCGEVPIFNLASCSLYSRSTVADIVLPWIMAGERVDLDDL
                     AEIGFGGLLDRDMSFRFPPYDSDEVRDEG"
     gene            complement(669..>1502)
                     /locus_tag="VHR64_05065"
     CDS             complement(669..>1502)
                     /locus_tag="VHR64_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3302230.1"
                     /translation="SYTVGLVTTDYFGRFNMPVIAGIEDALGAAEILVFLCTVRDDPV
                     RERNVVASLIAKQVDGIIVMGRRTDPRPPISVGQSGIPVVYAFSRVTGGDAFCLAPDE
                     DQGACLATDHLIRLGRRHLAHITGPSERESVELRLEGMRRTVLDHGLDWSDERCLSGE
                     WSEEWGYEAVNQLLDVDPAVDGIVCGSDIIARGVLDGLRERGRCVPDDVSVVGFDNWE
                     IVAEHSRPPLTTIDMNLHDMGHEAARRLLARLEGDRTSGTIRLPCSLIVRQSCGATSI
                     S"
BASE COUNT          269 a          475 c          483 g          275 t
ORIGIN      
        1 ctggcaatcg ctgccgggag agcggtggtc gagatccgcc cgttcctgcc tcggtcagtg
       61 gcggtgatca gcaccgaggg gctcgaggat gccgtgcggg agcggttccg gacagcggtc
      121 aacaagaaga tcgcctggta tggcagcagt gtcagcggct ttaccgatct gcccgacgat
      181 tccgatcagg tcgcgcaggc gatcgaggcg gcgatcgggg atggcgccgg gttgattttg
      241 accggcggtg gcaattcgct cgatccgctc gatccaaccc tggtcgccct ggaggcgatg
      301 ggcgcggaga tgatcaagtt cggggcgccg gcgcacccgg gcagcatgtt ttggctggcc
      361 cgctgcggcg aggtgccgat cttcaatctc gcctcatgct cgctctattc gcgctcgacc
      421 gtagccgata ttgttctgcc gtggatcatg gccggcgagc gggtcgatct ggacgatctc
      481 gccgagatcg gctttggcgg tttgctggat cgggatatga gtttccgctt cccgccatac
      541 gactcggacg aagtgcggga cgaggggtaa cgctcagggg cgaaccggcc ctcctcggct
      601 tatttccctc gaactggaca tcgatctcaa acccttcgtc caggccgtct aagatgtacg
      661 accaggcgtt agctaatcga cgtcgccccg cacgattggc ggacgatcag gctgcacggt
      721 aggcgaatcg tgccgctcgt acggtcgcct tcgagccgcg ccagtaaacg ccgtgctgcc
      781 tcgtgtccca tgtcgtgtag attcatgtcg attgtcgtca gcggcggccg ggagtgctcc
      841 gcgacgatct cccagttatc gaacccgacg accgaaacgt cgtccggcac gcagcggcca
      901 cgttcgcgta gtccatcgag cacaccgcgc gcgatgatgt cgctgccaca gacgatgcca
      961 tcaacggcgg ggtcaacgtc gagcagttga ttgacagcct cgtaccccca ctcttcgctc
     1021 cattcgccgc tcaggcagcg ctcatccgac caatcgagcc cgtggtcgag cacagtacgc
     1081 cgcataccct ccaggcgaag ctccaccgac tctcgctcag acggccccgt gatatgcgcc
     1141 aggtggcgac ggccaaggcg gatcagatgg tcggtggcga ggcaggcgcc ctggtcttca
     1201 tcaggcgcca ggcagaaggc gtcccccccg gtcacacggg agaacgcgta aacgactggt
     1261 attccggact ggccgacgct gatcggcggg cgcggatcag tgcgccgacc catgacgatg
     1321 attccgtcca cctgcttggc gatgagcgag gcgaccacat tacgctcgcg caccgggtca
     1381 tcgcgtacgg tgcagagaaa gacaagaatc tccgcggcgc cgagcgcgtc ctcgatgcca
     1441 gcgatgaccg gcatattgaa acggccgaaa tagtcggtcg tgaccaatcc gaccgtgtaa
     1501 ct
//
LOCUS       DATAOZ010000188         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4813824, whole genome shotgun sequence.
ACCESSION   DATAOZ010000188 DATAOZ010000000
VERSION     DATAOZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4813824"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VHR64_05070"
     CDS             complement(<1..285)
                     /locus_tag="VHR64_05070"
                     /inference="COORDINATES: protein motif:HMM:NF015884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEX3302231.1"
                     /translation="MTTPADVMKMAKDNGVKIFDFRFCDLLGTWQHTSKSISDVDEEI
                     FEHGVGFDGSSIRGFQSIDASDMLMMPDPTTARLDPYTAVPTMYVVCDLYD"
     gene            complement(637..2049)
                     /locus_tag="VHR64_05075"
     CDS             complement(637..2049)
                     /locus_tag="VHR64_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3302232.1"
                     /translation="MTLLQSLPDHLRSVLHALADAFEADGAELALVGGPVRDLLLERD
                     VTDLDLTTDAIPDRIKRLGDRAGASASFDMGERFGTIGFVFERDHAAPIDVEITTYRA
                     EYYPNESRHPEVTFGTSLTDDLSRRDFTINAMAIDLETDELIDPFEGQRDLHQSRIRA
                     VGDARSRFHEDPLRLLRAARFVGQLGFFIEPETKAAMTELAPELRRISRERIYAELTR
                     LLCGEFASHGLEVLLDTGLFQVAMPELKPLADEAEIERTIHREKDLWEHTLRVIDKSP
                     ARPIVRWAALLHDAAKPHTRSVSPDGQVHFFGHERVGAELAATLLRRLGADKHTVTSV
                     ARLVELHLRPAAYDVDWTDSAVRRLVLEAGDELADLLDLAGSDITSANERKIKQASRL
                     MSSLRSRIARLEEETALAELKSPLDGVELMELFQQPPGRWIADVKDHLRELVIEGILT
                     AGDKDRAREIAEEYLNLPGE"
BASE COUNT          375 a          741 c          796 g          417 t
ORIGIN      
        1 gtcgtagagg tcgcagacga cgtacatggt cgggacggcg gtgtaggggt caaggcgggc
       61 ggtggtcggg tccggcatca tcagcatgtc ggaggcgtcg atcgactgga agccgcggat
      121 cgaggagcca tcgaagccga cgccgtgctc gaagatctcc tcatcgacgt cggagatcga
      181 cttcgaggtg tgctgccaag tgcccagcag gtcacagaag cggaagtcga agatcttcac
      241 gccgttgtct ttggccatct tcatgacatc ggctggagtg gtcatctggt cgggtcctgc
      301 ctttcaccac aaaacgcaca ccgctccatc tgcggtgtgc aacgctgcgc gtaccgaagg
      361 tctcgtgccc aggtcctcgc tgacccgttg tccatacgtg ggcggccttc gtcgagttag
      421 attccggtcc cgctgaccgg aactgcctga cgaccattca tggtacgggc gagcgcagcg
      481 cgattctccg tgcaagacgc accaattgcc ggcgggtttt tgtgcatgtt gcacgaggcg
      541 agcaggcgag cagttaggcg cggagacggg aaggcgcgaa gtcggctact agaaactaga
      601 aacgagaaac tggaaacctt ctcccgaacc ccgaacctac tcccccggca ggttcagata
      661 ctcctcggcg atctcgcggg cgcggtcctt gtctcccgcg gtgaggatgc cttcgatgac
      721 cagctcgcgg aggtggtcct tgacgtcggc gatccagcgg ccgggtggct gctggaacag
      781 ctccatcagc tcgacgccgt cgagcgggct cttcagctcg gcaagcgccg tctcctcctc
      841 cagcctggcg atccgtgacc gcaatgagga catcagccgg ctcgcctgct tgatcttgcg
      901 ctcgttggcc gacgtgatgt cggagccggc cagatcgagc aggtcggcca gctcatcgcc
      961 cgcctccaac accaggcgac ggaccgcgct gtcggtccag tcgacgtcgt aggcagccgg
     1021 gcgcaggtgc agctcgacca gccgggcaac cgacgtcacg gtgtgcttgt cggctccgag
     1081 ccggcggagc agcgtcgcgg ccagctcggc tccgacccgc tcgtgcccga agaagtggac
     1141 ctgcccgtcc ggtgagaccg agcgggtgtg cggcttggcg gcatcgtgca gcaaggcggc
     1201 ccagcgcacg atcggccgcg ccggtgattt gtcgatcacc cgcagcgtgt gctcccagag
     1261 gtccttctcg cggtggatcg tccgctcgat ctccgcctcg tcggccagcg gcttcaactc
     1321 cggcatcgcg acctggaaca ggccggtatc gagcagcact tcgaggccgt gcgaagcgaa
     1381 ctcgccgcag agcagccggg tcagctcggc atagatccgc tcccggctga ttcggcgcag
     1441 ctccggagcg agctcggtca tcgccgcctt ggtctcgggc tcgatgaaga agccgagctg
     1501 cccgacgaat cgcgccgccc gcagcaggcg gagcggatcc tcatggaagc ggctgcgcgc
     1561 atcgccgacg gcccggatcc gcgactggtg caaatccctc tgcccctcga acgggtcgat
     1621 cagctcatcc gtctcaaggt cgatcgccat cgcgttgatc gtgaaatcgc gccggctgag
     1681 gtcgtcggtc agcgacgtgc cgaaggtgac ctccgggtgg cggctctcgt tcgggtagta
     1741 ctcggcgcgg taggtcgtga tctcgacatc gatcggggcg gcgtggtccc gctcgaagac
     1801 gaagccgatc gtgccgaacc gctcgcccat gtcgaagctg gcgcttgccc cggcccgatc
     1861 gccgagccgc ttgatccggt ccgggatcgc gtcggtggtc aggtcgaggt cggtcacatc
     1921 ccgctcgagc aggagatcgc ggaccggtcc gccgaccagg gcgagctcgg cgccgtcggc
     1981 ctcgaaggca tcggcgaggg cgtgcaggac agatcggagg tggtcgggca ggctctggag
     2041 caatgtcatg cgtctggtgg ttagagattg gtgattggtg gatagtggtt ggtggttggt
     2101 ggttggttgc tcacgcacct gcctcgctct attccacttt cctgtcgacg tgggcggctt
     2161 cattccgggc gagttctgaa cgggacccgc cgggatgacc gatcccgtca gatcagtcta
     2221 ctgaaccgcc ggcggcgcgg gcagattggg cctcggcctc ggcccgcatc gcgaggcgat
     2281 cgatcggtgc gaccagcggg cggtcgaccg gcacgatctc cggggacat
//
LOCUS       DATAOZ010000189         5206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2183472, whole genome shotgun sequence.
ACCESSION   DATAOZ010000189 DATAOZ010000000
VERSION     DATAOZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183472"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="VHR64_05080"
     CDS             complement(<1..1418)
                     /locus_tag="VHR64_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302233.1"
                     /translation="MSRKRKGGTSRDPTPFDLASAELHRHPLFAPLTHRIGLARSTDD
                     GWAADSWLWVSDYGMLRAHPHRVGSIDEWTYVLAHGLLHFGFGHLVVRPNQSAWNVAC
                     DYAVARFLADLRLGQKPPGINMPPDFPVRTEESLYQKYVEQGIPATANALGTAGVNVP
                     DMLLHSTLSKVERDMRARLWREAFAEGLVLAVTSAVNVAGGAEPFLGSRARRDSRAER
                     ARQWFINSYPLLGAMAASFTVYEDPHLCRRMAISVAAVHEERREIYINPGAGLTELEC
                     RFVMAHELLHVGLRHTARSEGRDHFLWNVACDYVINGWLIEMEVGDVPTVGLLHDPAL
                     KGESAESVYDRIATDLRLARRLCTLRGIGRGDMLEPSEDAWRNRAAGVELDEFCRRAM
                     ANGLTYHQDHCRGLLPAGLIEEINALAHPPIRWDVELARWFDHHFPPLERVRSYAHPS
                     RRQDATPDIPRPRYVPLPDDGQ"
     gene            complement(1408..2472)
                     /locus_tag="VHR64_05085"
     CDS             complement(1408..2472)
                     /locus_tag="VHR64_05085"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3302234.1"
                     /translation="MNAAVTVTQKQLAEFLLNIAPMRPVFIWGPPGIGKSSLVQLFAS
                     SVGLPCVSLLGSQLAAEDLIGVPQILDGKSRFCPPRMIAREDPYCLFLDELNACSHEV
                     QKAFYSLIHERRIGEYCLPPGSIVIGAGNRAHDSAIVKPMSSALLNRMVHVHLKVSHR
                     DWLEWAGQNGIHPLVLEYIQLRPDHLWSEPPKHEEPFSTPRAWHMMSDALAEFGDDVS
                     DTWLEVLAYGCLSPRHAGQFKAFVRQVRSEYRLSAILKGDKGWPRDPGSRDVLYFLAQ
                     SFRAQLIKELPAEKAGLGGDRSNLPHRAKALLKDLASISLEIAQLVVARDDDGTALPG
                     WFLSEVVRDLPRLVERKNVA"
     gene            2745..3857
                     /locus_tag="VHR64_05090"
     CDS             2745..3857
                     /locus_tag="VHR64_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302235.1"
                     /translation="MRLRISRIALLLWLVSSLAWTSAPVQAAESDQADRSLVEHSGEI
                     FFGTIDIVTAVFPVTTDAEPAMNTEYTVLISDVLKGNLRPQTMVVVRQDGGDADFADG
                     DGPLAPGAEYLFFTYYDPALLRYVIVEPAIGNVAITSPEQRDELTAHWSAVVQQTACG
                     YTDVLKLNGVVYAKRDWNDDKRYLEREQVGPTIDTVDRQDTTATGCRDDLVDRTASVI
                     PAGTKVQSVDGYAPTFRVAVRLPDRHRYLYEAIWSENAKTGADLLDIHDRVTSITWGD
                     SADCPDKAVCDIASVETKDSAVIARVVDLILDAPVGPVTFRVTEDLSNGISLLFGLKD
                     GSTAYIYFLATRGSSQSGIQVPVEELIAALRNRAEQ"
     gene            3854..4345
                     /locus_tag="VHR64_05095"
     CDS             3854..4345
                     /locus_tag="VHR64_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3302236.1"
                     /translation="MTSAGETQSAIRVELVDASQAELVLRLAVEAFAEFRDTLVPPPG
                     ILVESVDDVARYIETGGAVIAWDCDVPVGSARFHPEPDHLYIGRVAVPPAYRRRGIAT
                     EMMRFLEDHARSIGFTETRVQVRQSLPSNVALYQSLGYIVRSADPHPRVPEAIVLTLI
                     KRL"
     gene            4423..>5206
                     /locus_tag="VHR64_05100"
     CDS             4423..>5206
                     /locus_tag="VHR64_05100"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3302237.1"
                     /translation="MTTPLKPGVHCVMATPFLPDESLDETSLATLIDYLIDGGCDGIL
                     ILGVLGEADKLSDIERDRMIAKSIELSAGRLQVSVGITHNSTKVTRDRAMACVEAGAA
                     AVMVSPPSGTAAGPALKEHFRRIAADLPIPFIIQDHPVSSGVKLPVEFIAGLFEHMPP
                     NSIVKLEDPPTAPKMKRLLELAPGFQLFGGLGGVSLLHELDAGSSGAMTGFALPELLV
                     AIVQAHQAGDRERARRVFEASLPLMVFEAQPGAGVALRKELLK"
BASE COUNT         1013 a         1710 c         1527 g          956 t
ORIGIN      
        1 aattggccgt catcgggcaa tggaacatat cgtggccggg gaatgtccgg cgtcgcatcc
       61 tggcggcggc tgggatgagc ataggagcga acccgttcga gcggcggaaa atggtgatcg
      121 aaccaccgcg ccagctcgac gtcccaacgg atcggcgggt gagccagcgc attgatctcc
      181 tcgatcaggc cggctgggag cagaccgcgg cagtgatcct ggtgatacgt cagcccgttc
      241 gccattgccc ggcggcaaaa ctcatcgagc tcgacgcccg cagcgcggtt tcgccacgca
      301 tcctcggacg gctcaagcat gtcgccgcgg ccgatcccgc ggagagtgca cagtcttcgg
      361 gcaagccgga gatccgtcgc gatgcgatcg tacaccgatt cggctgactc gcctttcagt
      421 gctggatcat gaaggaggcc caccgttgga acatcgccga cctccatttc aattaaccat
      481 ccattgatta cgtagtcgca cgcgacgttc caaagaaaat ggtcccgccc ttcagaacgc
      541 gcggtatggc gtaacccgac atgaagcagc tcgtgggcca tgacaaaccg gcactcgagt
      601 tcagtcaaac ccgcgcccgg attgatgtaa atctcgcgac gttcctcgtg cacagcggca
      661 accgagatcg ccattcgccg gcaaagatgc gggtcctcat agaccgtgaa tgaggctgcc
      721 attgctccca gtagcgggta actattgata aaccactggc gcgcacgctc ggctcttgaa
      781 tcgcgcctgg cccggctccc gaggaatggt tcggcaccac cggcgacatt cacagcactc
      841 gtgacggcga gaacgagtcc ttcggcaaat gcttctctcc acaatcttgc gcgcatgtcg
      901 cgttccacct ttgacagtgt cgagtgaagg agcatgtccg ggacgttaac gccggccgta
      961 ccgagcgcat tcgctgttgc aggaatgcct tgctcaacat atttctggta gagactctcc
     1021 tcggttcgca ccgggaagtc aggtggcatg ttgatgcccg gaggcttttg ccccagcctt
     1081 aggtcggcga gaaatctggc gaccgcgtaa tcgcacgcga cattccaggc gctctgattt
     1141 ggtcgcacaa ccaggtgtcc gaatccgaag tgcagaagcc catgtgccaa cacgtacgtc
     1201 cactcgtcga tcgagcctac ccgatgcgga tgcgctctta acatcccgta gtcgctgacc
     1261 cacaaccagg aatcggctgc ccagccgtca tcggttgacc gggcgaggcc gatacgatgc
     1321 gtcaatggcg cgaagagggg atgccggtga agttcggccg aggccaaatc aaacggagtt
     1381 ggatctcgcg aagtgcctcc tttgcgttta cgcgacattc ttccgctcca ccagccgagg
     1441 caaatcacgg actacttcgc tcagaaacca gccgggcaac gccgtgccgt catcgtcacg
     1501 agcgacgacg agctgggcga tttccagact gatgcttgcc agatccttca gcagtgcttt
     1561 cgcccggtgg ggaaggttgg agcggtcgcc acctaaaccg gctttctccg caggtagctc
     1621 cttgatgagt tgcgccctga acgactgggc aaggaaatag agcacatcac gcgaaccagg
     1681 atcgcgcggc caccccttgt ctcctttgag gattgccgac aagcggtact cgcttcgcac
     1741 ctgccgaaca aacgccttga actgcccggc atgccggggc gaaaggcagc cgtatgcaag
     1801 cacctccaac caggtgtcgc tgacatcgtc cccaaactca gcgagcgcgt cactcatcat
     1861 gtgccatgct cgcggagtag agaagggctc ctcgtgtttc ggcggttcgc tccaaaggtg
     1921 gtcggggcgc aattggatgt actccagcac cagcgggtga atgccatttt ggcccgccca
     1981 ctccagccaa tcgcggtgag acaccttgag atgcacatgc accatgcggt tgagcaaggc
     2041 cgacgacatc ggtttgacaa tcgcactgtc gtgggcgcgg ttcccggccc cgatcacaat
     2101 cgatccgggc ggcaaacagt actctccgat ccgtcgctca tggatcaagc tgtaaaacgc
     2161 cttctgaact tcatgtgagc acgcgttcaa ttcgtccagg aagaggcagt acggatcctc
     2221 tctcgcgatc attcgcggag gacagaagcg gcttttcccg tcgagaatct ggggcacacc
     2281 aatcaggtcc tcggcagcca actgactgcc aagcagcgaa acgcaaggca gcccgactga
     2341 gctggcgaaa agctgaacca gcgatgactt tccgatgcca ggcggacccc agataaatac
     2401 gggacgcatg ggggcgatgt ttaagagaaa ttcggccagc tgcttttgcg tgaccgtaac
     2461 ggccgcgttc atgaagacac gatccttcca ggaccaatca atcgtccaag gacaacgcac
     2521 actgattttt cacgaattgg tagctggcaa catctcttat acaggtacgg ctcctctcct
     2581 gcaatatgag attggtcgct tccatagagg agatcgcgtt gggttacccg ggcaggtgtt
     2641 acacctctgt ctggctcctg gcccgcaatg agcgtccggc gcacgacgac cctggttcga
     2701 cggtcccggc cgctagactt gagtcaatgc cccggaggtt gtcgttgcga ctcaggatct
     2761 cccgaattgc gttgttgctc tggctggtca gcagcctggc gtggacgagt gcgcctgtcc
     2821 aggcggctga gagcgatcag gccgatcgat cgctggtcga gcactccggc gagatcttct
     2881 ttggcacgat cgatatcgtg accgccgtct ttcccgtcac gacggacgcc gagcctgcca
     2941 tgaataccga gtacacggtg ctcatctcgg acgtcctcaa gggcaacctg cgaccgcaga
     3001 cgatggtcgt cgtccgacag gacggcggcg acgccgactt cgcagacggg gacggcccgc
     3061 tcgcgcccgg tgccgagtac ctcttcttca cctattacga cccggccctg ttgcggtacg
     3121 tcatcgtcga gccggcgatc ggcaacgtcg cgatcacctc gccggagcaa cgcgacgaac
     3181 tgactgcgca ctggtctgcg gttgtccagc aaacggcctg cggctacacc gacgtcctca
     3241 agctgaatgg cgtcgtctac gccaagcgcg actggaacga cgacaagcgc tatctcgaac
     3301 gggagcaggt cggcccgacg atcgacaccg tcgaccggca agacaccacc gcgaccggct
     3361 gccgggacga tctggtcgac cgcactgcct cggtcattcc tgccggcacg aaggtccagt
     3421 ctgtcgacgg ctacgcgccg acattccggg tcgccgtccg cctgccggac cgccaccgct
     3481 acctctacga agccatctgg agcgagaacg cgaaaaccgg cgccgacctc ctcgacatcc
     3541 acgaccgcgt cacgtcgata acctggggcg acagcgccga ctgtcctgat aaagcggtct
     3601 gtgacatcgc ttcggtggag actaaggact cagcggtgat cgcacgagta gtcgatctga
     3661 tcctcgatgc gccggtcgga cccgtgacat tccgcgtcac cgaagatttg agcaacggta
     3721 tctcgctcct gttcggactt aaggacgggt cgaccgcgta catctatttc ttggcaacca
     3781 ggggatcatc acagtcgggt attcaggtgc ccgtagaaga gctgatcgcc gcactgagga
     3841 accgggccga acagtgacct cagcaggtga aacgcagtcg gcaatacgag ttgagctggt
     3901 cgatgcgtcc caggcggaac tggtcctgcg cctggccgtc gaggcattcg ccgagttccg
     3961 cgacaccctc gtcccaccac ccggcatttt ggtggagtcc gtcgacgatg tcgcgcgata
     4021 cattgagacc ggcggagcgg tcatcgcctg ggattgcgat gtgccggtcg gctcggcccg
     4081 cttccacccg gagcccgacc acctctatat cggtcgggtg gccgtcccgc ctgcgtatcg
     4141 ccgccgcggc attgccaccg agatgatgcg tttcctcgag gaccatgccc gctccatcgg
     4201 cttcaccgaa acccgcgtcc aggtccgcca gtccctcccc agcaacgtcg ccctctacca
     4261 atccctcggc tacatcgtgc gcagcgccga tccccatccc cgcgtcccgg aagccatcgt
     4321 cctcaccctt atcaagcgcc tctgacgccc ggattgccca aaccggtgcg tcctctgcga
     4381 tgatgtgcgc atgcggctga cgtacaccac ggagacaacg acgtgacgac cccactcaag
     4441 cccggcgtcc attgcgtgat ggcgacgccg ttcctgcccg atgaatcgct cgatgaaacc
     4501 agcctcgcca ccctgattga ctacctcatc gacggcggct gcgacggcat cctgatcctc
     4561 ggcgtgctcg gcgaggccga caagctctcc gacatcgagc gtgaccgcat gatcgccaaa
     4621 tcgatcgagc tttcggccgg ccggctccag gtctcggtcg ggatcacgca caattcgacc
     4681 aaggtcaccc gggatcgcgc catggcctgc gtcgaagcag gggcggcagc cgtcatggtc
     4741 tcgccaccgt cgggcaccgc cgccggacca gccctgaagg agcacttccg gcgaatcgcg
     4801 gccgacctgc ccatcccgtt catcatccag gaccacccgg tctcgagcgg cgtcaagctg
     4861 ccggtcgagt tcatcgccgg tctcttcgag cacatgccgc ccaactcgat cgtcaagctc
     4921 gaggacccgc cgaccgcgcc caagatgaaa cggttgctcg agctcgcgcc cggattccag
     4981 ctcttcggcg ggctcggcgg cgtctcgctc ctccacgagc tcgacgccgg ctccagcggc
     5041 gcgatgaccg gcttcgccct gccggagctg ctggtcgcga ttgtccaagc gcaccaggcc
     5101 ggagaccggg agcgggctcg ccgggtcttc gaggcttccc tgccgctgat ggtcttcgag
     5161 gcgcagccgg gcgccggcgt cgccctgcgc aaggagctac tcaagc
//
LOCUS       DATAOZ010000190         5492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6656803, whole genome shotgun sequence.
ACCESSION   DATAOZ010000190 DATAOZ010000000
VERSION     DATAOZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6656803"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VHR64_05105"
     CDS             complement(<1..359)
                     /locus_tag="VHR64_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3302238.1"
                     /translation="MDTPSYIVADLPAHARVVVVGAGIVGVSAAYELTKRGVSDVVVI
                     DRGDLFRTGGSTSHAPGGVFQNNSVRTVSKLAQWSVETFLDANNDGDTPVYWPTGSLE
                     VATTEARWQDLHRKYGYA"
     gene            837..1016
                     /locus_tag="VHR64_05110"
     CDS             837..1016
                     /locus_tag="VHR64_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302239.1"
                     /translation="MTVISIFRDEAGAEAGWEEITGSLHEVMEGQLEFLERESGPVDD
                     LLQIGCGPICWPLDD"
     gene            1009..2100
                     /locus_tag="VHR64_05115"
     CDS             1009..2100
                     /locus_tag="VHR64_05115"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX3302240.1"
                     /translation="MISAEIIRLRLHRHGLSAPGDLTPTDVVRRQGAMQSQEFGPAKW
                     SIGQRIAGATEPEVSRAFDVGEILRTHVLRPTWHFVAPADIRWLLKLTGPRVHKGNAG
                     RYRELGLDAETLAKSDATIAAAVEGGRFRTRTDLADYLNCAGIATDGQRIAYILMHAE
                     LEGLICSGPVRGKHQTYGLISERAPYETSLPRDEALAELVSRYFTSHGPATIADFTRW
                     SSLTVADTKRGIELLGDALQSTTIDGRTFWFGELPAEIATEPSPTAHLLQAYDEYVNG
                     YGESRDVVDPFIHRTFRANGYMQIILIDGRVAGAWKRTVRSMGIDVEIHPFRALTPAE
                     HDAIHRAAEAHARFAGLPAQVRFPVIPED"
     gene            complement(2097..2417)
                     /locus_tag="VHR64_05120"
     CDS             complement(2097..2417)
                     /locus_tag="VHR64_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302241.1"
                     /translation="MILVRTVFQGKFGTGGALAAAMSKGNEEFADYMEKAVGARRKWR
                     VLSDLSGPFDTVIFEVEAESLGEWQKARDAMFQMPEFGESMKETADMIMGGRNEYWTI
                     EAEG"
     gene            2645..3745
                     /locus_tag="VHR64_05125"
     CDS             2645..3745
                     /locus_tag="VHR64_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX3302242.1"
                     /translation="MGATAKHDKVKSEQVTLQTIADQLGVSRTTVSNAFSKPDQLTPK
                     LRAKIMKVADELGYCGPDPVARTLRKGKSGSYGILLTESLSYAMTDPAAVILLQGIAE
                     VFDEKNAGLLVLPSRGDRSTGVEAARNAVVDGFLIYSIAEDDPRLAVVQARNVPHVVI
                     EEPHLPGVASVSLNDREGARSIAEHVIGLGHRKLAALTFPLAEDDWTGLVSPERMAAS
                     TIRVSRERLSGYADAAAANGIDWAEVPVFEIVVNTIEEGRRGAGILLDQGERPTVILT
                     TADQLAIGAVQAARERGLQVPRDISIAGFDDIPAAALFDPPLTTVRQPLREKGALGAR
                     LLLDGWEGEPPSLVLPTELVVRASTGPAPALS"
     gene            3760..3984
                     /locus_tag="VHR64_05130"
     CDS             3760..3984
                     /locus_tag="VHR64_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302243.1"
                     /translation="MIGYQIIADVQRIAHLEAQSARPNSPVIPGPETGVRPPRIASLR
                     LQMTFALRRLADALEPAAMETIPNQKSQPC"
     gene            complement(4182..5174)
                     /gene="add"
                     /locus_tag="VHR64_05135"
     CDS             complement(4182..5174)
                     /gene="add"
                     /locus_tag="VHR64_05135"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX3302244.1"
                     /translation="MRDLRALPKAHLHLHIEGSARRSTIAEFAARTGRGYAPPASYPY
                     RDFSVFQAAYDEMTSYISTPEDLARICRELVEDDANQGAFYTEPIILPSFYQERFGMS
                     ELEIFDLMREAFFEAGEQCGIEVGIAIAGIFTLPIEATEAEARFAAEQSDRGVVAFNL
                     CATEPGFGYERWIRPCDIARDAGLDVVVHAGEFGPASAIEAAISVLNADRLSHGVRAV
                     DDPSVFALLAERGLVCDVAPTSNVVLGLYRELREVPILQFLDAGIPFTLNADDPLFSV
                     SGVGDEYVVVRDTFGLSDEEMAGIARTSVAASHSSPATKQRALDAIDTWLRTPA"
BASE COUNT         1025 a         1726 c         1761 g          980 t
ORIGIN      
        1 gcgtagccgt acttgcggtg cagatcctgc cagcgcgcct cggtggtcgc gacctcgagg
       61 ctgccggtcg gccagtagac cggggtatct ccgtcattgt tggcgtcgag gaaggtctcg
      121 accgaccact gggccagctt cgagacggtg cggaccgagt tgttctggaa gacgccgccc
      181 ggtgcatgcg aggtcgagcc gcccgtccgg aacagatcgc cgcggtcgat cacgaccaca
      241 tcggagacgc cgcgcttcgt caactcgtac gcggcggaga ccccgacgat cccggcgccg
      301 acgacgacga cgcgggcgtg ggctggcagg tcggcaacga tgtaactggg cgtgtccatg
      361 gtggtccccg gcggtgtgat ggcggattcg tctgcgcatt ttaggcgagc aggcgagaag
      421 tcgagcagcc gagcagtagg ttaagagttg agggtggtgg agcgagtacc caaaacctaa
      481 gacctaaaac ccaacccctc tttcgcgctg gacttgccgg gctaaaggtc tggcggaatc
      541 ggggacttct tcggcagtcg cggaaacaaa tgtttgtatt atcatccgta tctctgggga
      601 gatgccgacc acattcgaag gggcatgggt tagcaaatac cgccgtgcat gcgaacgtca
      661 caatctggtt gatcacggaa gttgcccgaa aatccgacag tatccaggcc tttttgcaaa
      721 cctggcggcc tggcggatat cgcgacttct ttcggtatgc cgcgccaaag atcattcccg
      781 tccttcgttc ccatgggttg ctcttctcgt tcgccatccg aacacaggca gaccgcttga
      841 ccgtgatcag tatcttccgg gatgaagcag gtgccgaggc gggctgggag gagatcactg
      901 gcagcctcca tgaggttatg gagggccagc tcgagttcct ggaacgcgag tcggggccgg
      961 tcgatgactt gctccagata ggctgcggtc cgatctgctg gccgctggat gattagtgcc
     1021 gagatcattc ggctgcgcct gcatcggcac ggtctctcag cgcccggcga tctgacaccg
     1081 accgacgtcg tgcgccgcca aggtgcgatg cagtcgcagg agttcggccc ggccaagtgg
     1141 agcatcggcc agcggattgc cggcgcgact gaaccggagg tgagccgggc gttcgatgtc
     1201 ggcgagatcc tgcgcactca cgtcctgcgg ccgacctggc acttcgtcgc gcccgcagac
     1261 atccgctggc tgctgaaact gaccggtccg cgcgttcaca agggcaatgc cggccgatac
     1321 cgcgagttgg ggctggacgc ggagaccctc gccaagtccg acgcgacgat cgctgccgca
     1381 gtcgaaggcg gtcgattccg gacgcgcacc gacctggccg attacctcaa ttgcgcggga
     1441 atcgccaccg acggtcagcg gattgcatac atcctgatgc acgccgagct cgaggggctg
     1501 atttgcagcg ggccggtccg aggcaagcac cagacgtacg gcctgatcag cgaaagagcg
     1561 ccatacgaga cctcactgcc gcgagatgag gcgctggcgg agctggtcag ccgctacttc
     1621 acaagccacg ggccggccac gatcgccgac ttcacacgct ggagcagcct gaccgtggcc
     1681 gataccaaac gcgggatcga gctgctcggc gacgcgctgc aaagcacgac aatcgacggc
     1741 cggaccttct ggttcggcga gttgccggcc gagatagcga ccgagccgtc cccgacggca
     1801 cacctgctcc aggcctacga cgagtatgtc aatggctacg gcgagagccg cgatgtcgtc
     1861 gatcccttca tccaccgcac cttccgggcg aatggttaca tgcaaatcat cctcatcgac
     1921 ggccgggtcg ccggtgcctg gaagcgcacc gtccgctcga tgggcatcga cgtcgagatc
     1981 caccccttcc gcgcactcac tcccgccgag cacgatgcaa tccaccgagc cgccgaagcg
     2041 cacgctcgct tcgccggttt gccagcgcag gtccgatttc ctgtgattcc ggaggactag
     2101 ccttcggctt cgatcgtcca gtactcgttg cggccgccca tgatcatgtc ggccgtctcc
     2161 ttcatcgact cgccgaactc gggcatctgg aacatggcgt ccctggcctt ttgccattcg
     2221 cccaggcttt cggcctcgac ctcgaagatg acggtatcga aggggccgga gagatcgctc
     2281 agcacccgcc acttgcggcg cgcgccgacc gccttctcca tatagtcggc gaactcctcg
     2341 ttccccttgc tcatcgcggc cgccagcgcg ccgcccgtgc cgaatttgcc ctgaaaaacc
     2401 gtccggacca ggatcatcgc agtcctcctt gatcgagacc aattgcggac tcgccacaaa
     2461 gcgcatgctt ggaatggcgg tcgcatccca gtctatgagc gcacgcaact gccatgtcag
     2521 gtgttcaagc caggttccga gtcgatcaat tgctccaagt ccgtgtgcgc aacctattga
     2581 caatgagcgg tattttcggt actgtatcga tcaagtttct ttatcgatca agtgagcatc
     2641 tgcgatgggc gcgacagcga agcacgacaa ggtgaaaagt gagcaagtga cgctgcagac
     2701 gattgccgac cagctcggcg tctcgcgcac gacagtttcc aacgccttca gcaagccgga
     2761 tcagctcacg cccaagctgc gcgcgaagat catgaaggtc gcggacgagc tcggctattg
     2821 cggacccgat ccggtcgcgc ggaccctgcg caagggcaag agcggatcgt acggcatcct
     2881 gttgaccgag agcctctcct acgcgatgac cgacccggcc gcggtgatcc tcctccaggg
     2941 tatcgcagag gtctttgatg agaagaacgc cgggttgttg gtgctgccgt cacggggcga
     3001 tcgctcgacc ggtgtcgagg ccgcgcgcaa cgccgtggtc gatggcttcc tgatctactc
     3061 gatcgccgag gacgacccgc gtctggccgt cgtccaggcg cgaaatgtcc cgcacgtcgt
     3121 gatcgaggag ccgcaccttc cgggcgtagc gagcgtgtcg ctgaatgatc gcgagggcgc
     3181 tcgctcgatc gccgagcatg tgatcgggct tgggcacagg aagttggcgg ccctgacgtt
     3241 tccgctggcc gaggacgact ggaccggctt ggtcagtccc gagcggatgg cggcatcgac
     3301 gatccgagtc tcccgcgagc ggctatcggg gtatgcggat gctgccgcgg cgaacgggat
     3361 cgactgggca gaggtgccgg ttttcgagat cgtggtcaac acgatcgagg agggccggcg
     3421 cggcgcaggg atcctgctcg atcaaggcga acggccaacg gtgatcctga ccaccgccga
     3481 tcagcttgcg atcggcgcgg tgcaggcggc gcgggaacgc gggttgcagg ttccgcgcga
     3541 catttcgatc gcaggtttcg acgatatccc ggcagcggcg ctgttcgatc cgccgctgac
     3601 gaccgtacgg cagccgcttc gcgagaaggg cgctctgggg gcgcgactgc tgctcgatgg
     3661 ctgggagggt gagcctccga gccttgtatt gccgaccgag ctggtcgtgc gggcctcgac
     3721 aggtccggcg ccggccctga gttgagacgg gagaacatca tgattggata tcagatcatc
     3781 gccgatgtgc agcgaatcgc tcacctggag gcgcagtcgg ctcgaccgaa ctcgccggtc
     3841 atcccgggac cggagaccgg agtacgaccg cctcgcatag cgtcgctccg gttgcagatg
     3901 accttcgccc tgcgccgcct ggccgacgcc ctcgagccgg cggcgatgga aacaatcccg
     3961 aaccagaaga gtcagccctg ctgagcggac aaaccagtga tcgaacaacg aaccggagcc
     4021 ggcgcaatca cgccggctcc ggttcattgt tgattgcgca gcaagacccg acttatctga
     4081 ctcagccccg gagggcctta tggtggttca gcccgcgcct ttaaccgcgg gcgccgccgg
     4141 gaaaccggta cgtgtctgca acatgaatgt cccgggctgc tctacgcagg cgttctcagc
     4201 caagtatcga tggcatcgag cgcgcgctgt ttcgtggccg gactggaatg cgacgctgca
     4261 acactggtgc gggcgatacc ggccatctcc tcgtcgctca agccaaaggt gtcgcgcacg
     4321 acaacatact cgtcaccgac tccactcacc gagaacagcg gatcgtcggc gttcagcgtg
     4381 aatggaatgc ccgcatcaag gaattgcagt atcggcacct cgcgcagttc ccggtagagg
     4441 ccgagcacga cgttggacgt cggcgcgacg tcgcagacca acccccgttc ggccaggagc
     4501 gcgaacactg agggatcatc gaccgcccgg actccgtgcg acagccggtc cgcattcaag
     4561 accgagatcg ccgcctcgat cgccgaggcc ggaccaaatt caccggcgtg gaccacgaca
     4621 tcgagaccgg cgtcgcgcgc gatgtcgcac ggccggatcc agcgctcgta gccgaagccg
     4681 ggctcggtgg cgcacaggtt gaaggcgacc acgccgcgat ccgattgctc cgccgcgaat
     4741 cgggcctcgg cctcggttgc ctcgatcggc agcgtgaaga tgccggcgat ggcgatcccg
     4801 acctcgatcc cgcactgctc acccgcttcg aagaaggcct cccgcatcag gtcgaagatc
     4861 tcgagctcgc tcatcccgaa gcgctcttgg tagaacgacg gcaggatgat cggctccgta
     4921 tagaacgcgc cctggtttgc atcgtcctcg accagttcgc gacagatccg ggccagatcc
     4981 tccggtgtgg agatgtagga cgtcatctcg tcgtaggccg cctggaaaac ggaaaagtcg
     5041 cggtatggat atgaggccgg cggcgcgtag ccccggccgg tgcgggcggc gaactcggca
     5101 atggtcgagc ggcgcgcact tccttcgata tggagatgca ggtgggcctt gggcagggca
     5161 cgtaggtcgc gcatgaatcc tccgggagcg caagaagtac aaattcgggt tgaccgggaa
     5221 tggtagggga ccaaccccgc ttcggcacgg cctgatccac cgtccgtggc cctgcgtatc
     5281 agtcattgac agcacgtatt tgcttcgtaa agtggtttcg tcgcattgca cagactgcgt
     5341 tcgctgttgt gcccggtggg cttcgaaggt gagggaatgc cgtgaatacc gacgacaaca
     5401 aggccgtgat tcgcaagatg tacacggccg tagacgcaca tgatgtcgac gttatcgagc
     5461 aggagttact tgatcccgtc taccggttcc gc
//
LOCUS       DATAOZ010000191         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6129136, whole genome shotgun sequence.
ACCESSION   DATAOZ010000191 DATAOZ010000000
VERSION     DATAOZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6129136"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1693
                     /locus_tag="VHR64_05140"
     CDS             <1..>1693
                     /locus_tag="VHR64_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX3302245.1"
                     /translation="SQPILTDGDLKKIHAIETLVSAFRTDTLDATWPASEGSDGMAPA
                     LDRLCREATDAVLLGHNILILSDRATSAERVPIPALLAISAVHHHLIRQGLRTSTGIV
                     VETGEAREVHHFCALAGYGAEAINPYLAFATLEQIRVENDLPLSPETVQKNYIKGVGK
                     GILKVISKMGISTYQSYCGAQIFDAVGLASDFVEKYFTGTATTIEGVGLPEIAQEAVE
                     RHKSAYGDNPIYQDMLDVGGDYAFRLRGEDHAWTPQSVAKLQHAVRGNSHADYRAFAS
                     AINDQSERLLTIRGLMSLKFAEEPIPLDEVEPASEIVKRFATGAMSFGSISREAHTTL
                     AIAMNRIGGKSNTGEGGEEAERFKPLENGDSKRSAIKQVASGRFGVTTEYLVNADVLQ
                     IKMAQGAKPGEGGQLPGHKVDKAIAKVRHSTPGVGLISPPPHHDIYSIEDLAQLIHDL
                     KNANPQAAVSVKLVSEVGVGTVAAGVSKARADHVTIAGFEGGTGASPLTSLTHAGSPW
                     EIGLAETQQTLVLNGLRGRITVQVDGGLRTGRDVMIGALIGADEFGFSTAPLIAAG"
BASE COUNT          331 a          530 c          546 g          286 t
ORIGIN      
        1 tcgcagccga tcctgaccga tggcgatctg aaaaagatcc acgctatcga gaccctggtc
       61 agcgcctttc gcaccgatac gctggacgcg acttggccag cctccgaagg cagcgatggc
      121 atggcgccgg cgctcgaccg gctgtgtcgc gaggcgaccg acgccgtgct cctaggccat
      181 aacatcctga tcctgtccga tcgcgcaacc tcggccgaac gggttccgat cccggcgctg
      241 cttgccatct cggcggtgca ccatcatctg atccgccagg gcctgcgcac ttcgaccggc
      301 attgtcgttg agaccggcga ggcgcgggaa gtgcaccatt tctgcgcgct cgccggctat
      361 ggcgcggagg cgatcaaccc ctatctcgcc tttgccactc tcgaacagat ccgggttgag
      421 aacgacctgc ccctcagccc cgagacggta cagaaaaatt acataaaggg cgtcggcaag
      481 gggatcctca aagtcatctc gaagatgggg atctcgacct accagtccta ttgcggcgcg
      541 cagatctttg acgccgtcgg gctcgccagc gacttcgtcg agaaatactt caccggcacg
      601 gcgacgacga tcgaaggcgt ggggttgccg gagatcgctc aggaagcagt agagcggcat
      661 aaaagcgctt atggcgacaa cccgatttac caagacatgc tcgatgtcgg aggcgactac
      721 gcgtttcggt tgcgtggcga agaccatgcc tggacaccgc aatcggtggc gaagctccag
      781 cacgcagtgc gcggcaactc gcacgcggat taccgggcgt ttgcatcagc gataaacgac
      841 cagagcgagc ggttgctgac gatccgcggg ctgatgtcgc tcaagttcgc cgaggagcca
      901 ataccgctcg acgaggtcga gcccgcgagt gagattgtca aacgcttcgc aaccggcgcg
      961 atgagtttcg gttcaatcag ccgcgaggcg catacgacct tggcgatcgc gatgaaccgc
     1021 atcggcggca aatccaatac cggtgagggc ggagaagagg cggagcggtt caagccgttg
     1081 gagaacggtg attcgaaacg ctcggcgatc aagcaggtcg cctccggccg gttcggcgtg
     1141 accaccgagt acctcgtcaa cgccgatgtc ctgcagatca agatggccca gggcgcgaag
     1201 cccggcgagg gtgggcaatt gcccggccac aaagttgaca aggccatcgc caaggtgcgg
     1261 cattcgaccc cgggggtcgg gctgatctcg ccgccgccgc accacgacat ctactcgatc
     1321 gaggatctgg cgcagctgat ccacgatctg aagaacgcca atccgcaggc tgcggtgtcg
     1381 gtcaagctcg tctctgaggt cggcgtcggc acggtcgcag ccggggtttc gaaggcgcgc
     1441 gccgaccatg tcacgatcgc cggcttcgag ggcggcaccg gcgcgagccc gctgacctcg
     1501 ctgacccacg ccggctcgcc ctgggagatc gggctcgccg aaacccagca aaccctggtg
     1561 ctgaacgggt tgcgcggccg catcacggtg caggtcgatg gcgggctgag aaccggtcgt
     1621 gatgtcatga ttggagcctt gatcggcgcc gacgagttcg gcttttcgac ggcgccgtta
     1681 attgccgcgg gct
//
LOCUS       DATAOZ010000192         6241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6918941, whole genome shotgun sequence.
ACCESSION   DATAOZ010000192 DATAOZ010000000
VERSION     DATAOZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6918941"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VHR64_05145"
     CDS             complement(<1..406)
                     /locus_tag="VHR64_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX3302246.1"
                     /translation="MVFSRLFRRQTEADQQLSKGLEKTRRGVFSEIARLFDRGTFDDG
                     FYDELEMLLIQADLGWDISQQLVEDLTASVEEHGITDPQFAKDYLRSDMVRLLELATR
                     NRKVKILQRGVPFVIMVVGVNGVGKTTSIAKLA"
     gene            517..1227
                     /gene="radC"
                     /locus_tag="VHR64_05150"
     CDS             517..1227
                     /gene="radC"
                     /locus_tag="VHR64_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX3302247.1"
                     /translation="MTERRHMLAEMPEAERPRERLLRHGPSALSNIELLAILLNTGLP
                     GESVMVVAERLLREFGGFPGLMKLSAEELARIHGIGPAKATKLKASMEIANRILASNP
                     NQKPKISSPDDITNLIGLEMSLLEQEQLRIVLLNTRNEVIGIRTLYQGTTNQAQVRIA
                     EVFRDAVRANAVAIIVVHNHPTGDPTPSSADIELTRSMVEAGEMLEIRVLDHMIIGHG
                     RHVSMKRLGLGFSGPNGR"
     gene            1234..2022
                     /locus_tag="VHR64_05155"
     CDS             1234..2022
                     /locus_tag="VHR64_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3302248.1"
                     /translation="MERGYGVSALINPDLIGPLANAAEAAGYQTFWVNDVPNGNGLER
                     LSRAQQVTSRIGLGVGVLPVDRWNAQAIAEEIARLNLDPDRLVVGIGAGTLHKGSLDA
                     TADVASDLISRVRARVLIGALGPKMCRLAGSGAHGVLLNWLTPGAAEELALMTKEGAA
                     AVGRPVPEIVGYVRTAADPAAQSRLEKEATAYEGYPSYARHFERMGVRAIDTTVNGSG
                     SEIDRRFQAYASSTDEVVARAIAASESLDDYLAVLRAASPAAGA"
     gene            2022..2594
                     /locus_tag="VHR64_05160"
     CDS             2022..2594
                     /locus_tag="VHR64_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX3302249.1"
                     /translation="MEPFESREQALAWARDLVRRPDVLYLDTETTGVRFGFDDVIDIG
                     IVDGRGIVVMDQLVRPSVAIPGDSEAVHGISNRAVAHAPRLPEIWNQLSRILNGQVVV
                     SYNATFDEQMLADAGARRGLAPIIPARWDCAMEAFAAYNGEPSHHRPGFRWINLGAAA
                     RMLGLEHPEHRAVSDAMLCLELVQELSRRK"
     gene            complement(2580..4022)
                     /locus_tag="VHR64_05165"
     CDS             complement(2580..4022)
                     /locus_tag="VHR64_05165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3302250.1"
                     /translation="MSASFAYNSPVQAMRGLPSQWRLISGMTVIAFVVASALIVNGPL
                     SIGRAILLGIAAFAAVWAAAIFAGSTVARRRTDGVAWTAGVIATLVAILCVTASLNDD
                     GRARIVIAALGVSIAVMLWIYAVVNADRSAGFDASVATLALLMIAVGLLLIPPHFEEK
                     IHSDAGGLLIATLPSLAAAALITRRSRRGVILLLLIAVVAGLLIVQPDDLTSWLIGAA
                     IATLLAIADSHLPVRRNRTILSFTGTDAVLFGVASTFLGIYLAFSINEPVRGFQMGAL
                     AALAVAVTLVQRLIIDQRDERMLELATLTAQYRERAQLDSLTDLPNRAALDSRLLEEV
                     ERAVRYRHPMSLCFIDIDHFKAINDTAGHQVGDAILRGLAEVLQATVRTPDFVARYGG
                     EEFVVIAPGTWSADAAVLGRRIQAAVARGLPHPLGRPITVSIGIAGVPEHGREASAVL
                     RVADIALYAAKRAGRNRVEIGATHETSLPT"
     gene            4117..5343
                     /locus_tag="VHR64_05170"
     CDS             4117..5343
                     /locus_tag="VHR64_05170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302251.1"
                     /translation="MTTTSTPRSQLRRIVQTEAGGWGVLTVVNSAFIQPLLIASGAGQ
                     LALGVYISGSSLFSFGAGWVGPRLASRLGSTARATLSVVGIGRIVFLIFTAYLLFAGN
                     ARPEILIPLILIWALGEGLAVPLWNSFLAGMVGASERGRWVAMRGQAATIGTVPILIA
                     ILGVVLIASRERALPFAYAVAAGGAVVSWFALRRMFASSAPQPVPPKRSLKHVPESSE
                     ARRFLLGVFLFWFASALTWPIVPRYLVNDLHAPTAYFAITPIIGALIGIVVQPRWGKF
                     GDRSGAARILLMSGIGCAIVPFLWAIVPVYWLGFIVDSVAYVFWPGHALGLTLRAIEL
                     AENEAERPMMLGWTNLAQGIGATISPLIAAAVVGHLSVPVILLVACALRAASATVMSG
                     SAWRLTGKQAAVPTTA"
     gene            complement(5340..5645)
                     /locus_tag="VHR64_05175"
     CDS             complement(5340..5645)
                     /locus_tag="VHR64_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302252.1"
                     /translation="MTLPSENGQSQTTPQRASGQDGQGKGQTATRKFVFTAWGDSVDR
                     DLCAYALAELPERTPIAGRTGLATAEWVVVDRVDGRALICNRLHDGECAWPWAVESV"
     gene            5774..>6241
                     /locus_tag="VHR64_05180"
     CDS             5774..>6241
                     /locus_tag="VHR64_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX3302253.1"
                     /translation="MTKQIGIDLGTANVLVFVRGRGIVINEPSVVAISARDGRVKAVG
                     LEARNMLGREPRETIEVVRPMRDGVIADYVVTQEMLRYFIGKAMGRFSLIRPEVMICV
                     PAGVTGVERRAVRDAALNAGARRAYLISEPLAAAIGAQIPVADPSGNMVIDIGG"
BASE COUNT         1133 a         1917 c         2012 g         1179 t
ORIGIN      
        1 cggcaagttt cgcgatcgat gtcgtcttgc cgacgccatt cacgccgacc accatgatta
       61 cgaacggaac gccgcgctgc aggatcttga ccttgcgatt tcgcgtcgcc agctcgagca
      121 ggcgcaccat gtcgctgcga aggtagtcct tcgcgaattg cgggtcggta atgccgtgct
      181 cctcgaccga cgccgtcagg tcctcgacga gctgctggct gatgtcccag ccgagatcgg
      241 cctggatcaa caacatctcg agctcgtcgt agaagccgtc gtcgaaggtg ccgcgatcga
      301 agagccgggc gatctcgctg aagacgcccc gtctggtctt ctccagaccc ttgctcaatt
      361 gctgatcagc ttcagtttgt cgtcggaaga gacggctgaa aaccactgtg ctatgcctct
      421 gtcgaatgag aagtcggatt gagggttggc gtgtgagccg gtccatcgaa aagtatatca
      481 tcgacaggat ttcgcaccgc gtgcgatcat ctatttatga ccgagcgacg acatatgctg
      541 gcggagatgc ccgaggcgga acggccgcga gagcggctgc ttcgacatgg tccatccgcc
      601 ctgtcaaaca tcgagttgct ggcaatcctg ctgaataccg gactccccgg cgaatcggtc
      661 atggtcgtgg ctgagcgatt gctgcgcgag ttcggcggct ttccggggct gatgaagctg
      721 agcgccgagg agcttgcccg gatccacgga atcgggccgg cgaaggccac gaagctcaag
      781 gcatcgatgg agatcgcgaa ccggatcctg gcctcgaatc cgaaccagaa gccgaagatc
      841 tcaagccctg acgacattac caacctgatc gggctcgaga tgtcgctcct cgagcaggag
      901 cagctccgca tcgtgctcct caacactcgc aatgaggtga tcgggattcg cacgctctac
      961 cagggaacca cgaaccaggc acaagtccgg atcgccgagg tgtttcgtga cgcggtgcgg
     1021 gcgaatgcgg tggcaatcat cgtcgtccat aatcatccca ccggcgatcc aaccccttcg
     1081 agcgccgata tcgagcttac gcggtcgatg gtcgaagccg gggagatgct cgagatcagg
     1141 gtgctcgacc acatgatcat cgggcacggc cggcacgtct caatgaagcg gctcgggctc
     1201 ggattctcgg gtccgaacgg gcggtaggtg gcaatggagc gcggttacgg agtctcggca
     1261 ctcatcaatc cggatttgat cggaccgctt gccaatgcgg ccgaggcggc cggctaccag
     1321 accttttggg tgaacgatgt cccgaacggg aacgggctgg agcgattgtc gcgcgcacag
     1381 caggtcacga gccggatcgg gctcggagtc ggcgtgttgc cggtggatcg ctggaatgcc
     1441 caggcgatcg ctgaggagat tgcgcgtctc aatctggatc cggatcggct ggtcgtcggg
     1501 atcggcgccg ggaccctgca caagggttcg ctcgatgcaa cggccgacgt cgccagtgat
     1561 ctgatctcgc gagtacgggc cagggtcttg atcggcgcgc tgggaccgaa gatgtgtcgc
     1621 ctggccgggt ccggagcgca cggagtcctg ctcaactggc tgacgcccgg agcggccgag
     1681 gaattggcgc tgatgaccaa agagggcgcg gctgccgtcg ggcgtcccgt gcccgagatc
     1741 gtcgggtatg tgcgtaccgc cgctgatcca gcagcccaga gccggctcga aaaggaggca
     1801 actgcctacg agggataccc gtcgtacgcc cgccacttcg agcgtatggg tgtccgggcg
     1861 atagatacca ccgtcaatgg gtccggcagc gagatcgacc ggcgattcca ggcgtacgct
     1921 tcgagcacgg atgaggtcgt ggcgcgcgca atcgccgctt cggaaagcct cgacgattac
     1981 ttggcggtcc tccgtgccgc cagtccggca gccggagcgt gatggaacca ttcgaaagtc
     2041 gcgagcaggc gctcgcgtgg gcacgggatc tggttcgccg ccccgatgtg ctctacctgg
     2101 acactgagac gaccggcgtt cgcttcggtt tcgacgacgt gatcgacatc ggaattgtcg
     2161 acggccgcgg catcgtcgtt atggatcaac tcgttcgtcc ctcggtcgca atacccggcg
     2221 actccgaagc ggtccatgga atctcgaatc gtgccgtcgc ccacgcgccg cggctgccgg
     2281 agatttggaa ccagctctcg cgcatcctga acggacaggt tgtcgtgtcc tacaacgcca
     2341 cattcgatga gcagatgctg gccgacgccg gcgcgcgtcg ggggctggcg ccgatcatcc
     2401 ctgctcggtg ggattgcgcg atggaagcat ttgccgcgta caacggcgag ccatcgcacc
     2461 accgcccggg cttccggtgg atcaatctcg gcgcggcggc tcgcatgctg ggccttgagc
     2521 atccggagca tcgggccgta tcggacgcaa tgctttgcct ggagctggtt caggagctgt
     2581 cacgtaggaa gtgacgtttc gtgggtggcg ccgatctcga cccggttgcg gccggcccgc
     2641 ttggcggcgt agagggcgat gtcagcgacc cgaagcaccg cgctcgcctc gcggccatgc
     2701 tccggaactc cagcgatgcc gatcgacacg gtgatcggtc gccccagcgg gtgcggtagt
     2761 ccccgcgcca cggccgcctg aatacggcgc ccgagcacgg cggcgtcggc cgaccaggtt
     2821 cccggcgcga taaccacgaa ctcttctcca ccgtaacgcg ccacgaaatc gggcgtgcgc
     2881 acggtcgcct gaagcacttc cgcaaggcca cgcaggatcg catcgccgac ctggtgtccg
     2941 gcggtgtcgt tgatcgcctt gaagtgatcg atgtcgatga agcagagcga cattggatgg
     3001 cgatagcgca ccgcgcgctc gacttcctcc agcagacggg agtcgagcgc cgcccgattg
     3061 ggaaggtcgg ttaacgagtc gagctgggcc cgttcacgat attgcgcggt gagcgtggcc
     3121 agttccagca tgcgctcatc acgttggtcg atgatcaggc gctggacaag tgtgaccgcc
     3181 accgctagag ccgcaagcgc gcccatctgg aatccgcgca ccggctcatt gattgagaat
     3241 gcaaggtaaa taccgaggaa cgtgctggca acaccgaaca acacggcgtc cgtcccggta
     3301 aaggacaaga tagtccgatt gcgcctcacc ggcaggtggc tgtcagcgat agccagcaac
     3361 gtcgcgatgg ctgcgccgat gagccaggat gtgagatcgt ccggctgcac gatgagcaag
     3421 cctgcgacga ccgctatcag aaggagcagg atcactcccc ggcgggatcg ccgggtgatc
     3481 agcgctgcgg cagccaaaga cggcagcgtc gcgatcagaa gacccccggc gtcggaatga
     3541 atcttttcct cgaagtgcgg cggaatcagc agcaggccga ccgcgatcat cagcaatgcg
     3601 agcgtcgcaa ccgacgcatc gaacccggcc gagcgatccg cgttgaccac cgcatatatc
     3661 cagagcatga ccgcaatcga gacgcctaac gcggcgatca ctatccgggc gcgaccgtcg
     3721 tcgttgaggc tggcggtcac gcagagtatc gccacgagcg tcgcgatcac tcctgccgtc
     3781 caggcaacgc catctgtgcg tcttcgggcg acagtcgatc cggcaaaaat cgcggctgcc
     3841 cacactgccg caaacgctgc gatcccaagc aggatcgctc gtccaatcga aagtgggccg
     3901 ttcacaatca gggcggacgc cacgacaaat gcaatcacgg tcatgcccga aatcagccgc
     3961 cattgagacg gcagaccgcg catcgcctga accggtgagt tgtaagcgaa cgaagcggac
     4021 aatctggtga ttcctccggg tgaaggattg cctgttccta gagtaatcta cggaaaacag
     4081 tgtccgtacg ccagacccgg taccggggat accccattga ctacaaccag cacgccgcgt
     4141 agccagctcc ggcgaattgt tcagactgaa gccggcggtt ggggtgtgct caccgttgtc
     4201 aacagtgcct tcattcagcc gctgttgatt gccagcggcg ccggtcaact cgctctcggc
     4261 gtttacatct ccggatccag ccttttcagt ttcggagccg gctgggtcgg acctcgcctg
     4321 gcgagtcgtc tgggcagcac tgctcgagcg acgctgagcg tcgttggcat tggtcggatc
     4381 gtattcctga tctttaccgc atacctcctg ttcgccggta atgctcggcc cgaaattctg
     4441 atcccgctga tactgatctg ggcgctgggt gaggggctcg ccgttccact ttggaacagc
     4501 ttcctggctg gcatggtcgg cgcatcggaa cgcggtcgat gggtggcaat gcgcggtcag
     4561 gctgccacga tcggcacagt gccgatcctc attgccattc tcggagttgt cctgattgca
     4621 tcccgggagc gggcgctgcc atttgcatat gccgtagctg ccggaggggc cgtcgtcagt
     4681 tggttcgcgc tcaggcggat gtttgcgtcc tccgccccgc agcccgttcc accaaagcga
     4741 tcgctgaagc acgtccctga atcgagcgaa gcgagacgct tcctgctggg cgtgtttctg
     4801 ttctggttcg cgtccgcgtt gacctggcca attgtcccgc gctatctcgt aaacgacctg
     4861 catgccccga ccgcatattt tgcaatcact ccgataattg gcgcgttgat cggcatcgtg
     4921 gtgcagccgc ggtggggaaa gtttggagat cggtctggcg cggcacgaat cctgctgatg
     4981 agcggcatcg ggtgcgcgat cgtgccgttc ctatgggcaa ttgtgcccgt ctactggctc
     5041 ggattcattg tcgattcggt ggcgtatgtc ttctggcccg gtcatgcgtt gggactgact
     5101 ctccgggcaa ttgagctggc agagaatgag gcggagcgcc ccatgatgct gggttggacc
     5161 aacctggcgc agggaatcgg ggccacgatc tcaccgctga ttgccgccgc ggttgtcgga
     5221 catctgtccg tgccggtcat tctgctggtg gcgtgtgcat tgcgggcggc gagtgcgacg
     5281 gtaatgtcgg gcagcgcctg gagactgacc gggaaacagg cggcggtccc gacgactgcc
     5341 tagaccgatt cgacagccca cggccaggcg cattcgccgt cgtgcaggcg gttgcagatg
     5401 agcgctctcc cgtcgacccg atccactacg acccattccg cggtggcaag tcctgtcctg
     5461 ccggctatcg gcgtccgttc cggcagttcg gccagagcat aggcgcacag gtcacgatcg
     5521 acggagtcgc cccaggcggt gaaaacgaac ttgcgcgtgg ctgtctggcc cttgccctgg
     5581 ccgtcctgac cggacgcgcg ttgaggcgtg gtttgggatt gtccgttctc ggagggcaat
     5641 gtcatcctgc cattctgcaa ctgactatta ctgaccctat agtacacttg gctcctgcaa
     5701 ccggggcagt gccgccccat ccgacgcgca gtgcacgccc gtgccagcag cctggccggc
     5761 ggagattcta cctgtgacga aacagatagg catcgattta gggacggcga acgttctcgt
     5821 tttcgttcgt ggccgcggca tcgtgatcaa cgagccgtcc gtcgtcgcca tctcggcacg
     5881 tgacggccgc gtaaaggcgg tagggctcga ggcacggaac atgcttggac gcgaaccgcg
     5941 agaaaccatc gaagtcgttc ggccgatgcg agacggcgtg atcgccgact atgtcgtcac
     6001 ccaggagatg ctccgctatt tcatcggcaa ggcgatgggt cgcttctcgc tgatccggcc
     6061 cgaggtcatg atctgcgttc ccgccggggt gactggtgtc gagcggcgcg ccgttcgcga
     6121 tgccgcgctc aacgccggcg cccgtcgcgc gtatctgatc agcgagcctt tggccgccgc
     6181 gatcggcgcc cagattccgg tcgctgatcc ctcgggcaac atggtgatcg acatcggtgg
     6241 c
//
LOCUS       DATAOZ010000193         5040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9024717, whole genome shotgun sequence.
ACCESSION   DATAOZ010000193 DATAOZ010000000
VERSION     DATAOZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9024717"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VHR64_05185"
     CDS             complement(<1..308)
                     /locus_tag="VHR64_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX3302254.1"
                     /translation="MIRIKRAYEDASQDDGARFLVDRLWPRGVSKKALQIEAWLKDVA
                     PSTELRKWFGHDPAKWDEFQRRYITELESEPGAWHPLLDAADNGTVTLVYGAKDIEHN
                     "
     gene            complement(360..719)
                     /locus_tag="VHR64_05190"
     CDS             complement(360..719)
                     /locus_tag="VHR64_05190"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3302255.1"
                     /translation="MKGARLLHADLPIIPSPSGLPSQHIVTDQVGATSLFLGQQWLQP
                     GDEVFRHTHPCEEALMFLSGTGEASLGDETVTLEPGCSLFIPAGVEHGFRNTGETEMH
                     VVIVFPVPHFAPTTMVD"
     gene            complement(746..2125)
                     /locus_tag="VHR64_05195"
     CDS             complement(746..2125)
                     /locus_tag="VHR64_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3302256.1"
                     /translation="MRSADHRGDQPLSDWLGQWGPFAPDPSIDVPEAEVRKVLRKLAE
                     RLTDNYPFPHPVYAGQMLKPPHPVAAAAYAMAMQINPNNHALDGGPATAAMEREVIAT
                     MARMVGYWPETSLGHLTSSGTIANLEALWVARELHPGKAIASSAGAHYTHQRMCGVIG
                     VEHVAIPTEPSGRISIPHLREAVEAGQIGTVVATLGTTSLGAVDDLLITNGICRDHGV
                     RVHVDAAYGGFFRLLADGEALEPGVAAAFKAMGDADSIVIDPHKHGLQPYGCGSVLFR
                     DASVGRFYKHDSPYTYFTSCDLHLGEISLECSRAGAAAAALWATLKLFPLTPEGLGRI
                     LARTRQAATTWAALISDSRDLRLVTQPDLDIVCFYPTPARPMRVSDISAWTERIFVHG
                     MCPHPDAFFLAKLVMTEEQLDRSAHPNLIWDQKTITVLRSVLMKPEHADLVPALHERV
                     IESTKRTNV"
     gene            complement(2125..3525)
                     /gene="allB"
                     /locus_tag="VHR64_05200"
     CDS             complement(2125..3525)
                     /gene="allB"
                     /locus_tag="VHR64_05200"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX3302257.1"
                     /translation="MSDTVVIEGGTVVTHDGQFRADVVIANERITALTTDATDIDADH
                     RIDATGLLVLPGGIDVHTHFREPEEFTKEGFFAGGQGAAAGGITTVIEMPQADPTTIS
                     VEQFQAKRARVNKNALVDMALWAGIVGPPLQDEVDLQDLAGTGAVAFKSFMASSSPSF
                     PAVDTSTLYWAMAIVAETGMPYALHAEDNNLVQSGLKRLQDSGRADPIAHAESRPPLV
                     EAVAVAEALYLAEVTGCWVHICHCASADALRLIADARTRGVRVTVETCPQYLSLNTDD
                     LLALKGYGRCAPALRSQDEVDHIWSYVLDETVDLICSDHCGYTKEQKDAGEDDIFRAP
                     MGLAGIQTLLPVFYDGAVRGHSMNLSQFVRQIATNPAQVFDLYPRKGTIAIGSDADIV
                     VFDPERSWVARGEEMAHRQKWTPFEGKTITGRVVRTLRRGETIFDDSLEGEVRLPALA
                     GSGRFLPRGYGRAAVS"
     gene            complement(3547..3813)
                     /locus_tag="VHR64_05205"
     CDS             complement(3547..3813)
                     /locus_tag="VHR64_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302258.1"
                     /translation="MSRNTVTPAERALRDVGGPTYFEIGRVDPPENSVSAFAPDDWRL
                     YFLMRELPVADRIQVLRFAEMLYNLRHAMDWADDTPDEAPAHLT"
     gene            complement(3996..4259)
                     /locus_tag="VHR64_05210"
     CDS             complement(3996..4259)
                     /locus_tag="VHR64_05210"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX3302259.1"
                     /translation="MATQTMKTTVDTSMIKEHMPIVCSEGGQFGTVDHLDGNSIKVTK
                     DEQGQHHWIPLSWVTRVDDHVHVDRPGKQAMQEWFTSPPNGNR"
     gene            complement(4265..4477)
                     /locus_tag="VHR64_05215"
     CDS             complement(4265..4477)
                     /locus_tag="VHR64_05215"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX3302260.1"
                     /translation="MTDQSQDRLEGKFDEAKGQGKEALGKLADDKSTQMEGQKDQAMG
                     KAKQGMADAKDKVDDVVKDVTNRDNR"
     gene            4720..>5040
                     /locus_tag="VHR64_05220"
     CDS             4720..>5040
                     /locus_tag="VHR64_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302261.1"
                     /translation="MRNVEQLQIELREHLHSLEQSGVVGLSATFQPAEWGAIVPRLDH
                     DELVTLFEWLPEDDAATVLAALDPHTAADVLQVIGPVQAAAALDEMPEDDAADVLEAL
                     PDEHA"
BASE COUNT          880 a         1663 c         1560 g          937 t
ORIGIN      
        1 tcgttgtgct cgatgtcttt cgcaccatag accaacgtca ccgtgccgtt gtccgcggca
       61 tctaggaggg gatgccacgc ccccggctcg gactccaact cggtgatgta gcggcgctgg
      121 aactcgtccc atttcgccgg atcgtggccg aaccacttcc gtagctcggt gctcggtgcg
      181 acatccttca gccacgcctc gatctgcagc gcctttttcg acacaccccg cggccagagt
      241 cggtccacca ggaatcgcgc cccatcatct tgtgacgcgt cctcgtacgc ccgcttgatc
      301 ctgatcatta cagcctccct cgaacctaag ggtacgcggt tcgggcgggc tgcaacccgt
      361 caatccacca tcgtcgttgg cgcgaagtgg gggaccggga agacgatgac gacgtgcatc
      421 tcggtctcgc ccgtgttgcg aaaaccgtgc tcgaccccgg ccggaatgaa gagcgagcat
      481 ccaggttcga gcgtaaccgt ttcgtcgccg aggctcgcct cgccggtccc gctcaggaac
      541 atgagcgcct cctcgcacgg gtgggtgtga cgaaagacct catctccggg ctgaagccac
      601 tgctggccga ggaagaggga ggtcgccccg acctggtcgg tgacgatgtg ctgcgagggg
      661 agtccgctgg ggctcgggat gatcgggaga tcggcgtgca gaagtcgtgc acccttcatg
      721 gctcgcattc tcctccagcg gtcccttaca cgttcgtccg cttcgtggat tcgatgaccc
      781 gttcatgcag ggccgggacg aggtcggcgt gctccggctt catcaggacg ctgcgcagaa
      841 ccgtgatcgt cttctggtcc cagatcaggt tgggatgcgc cgaccggtcg agctgctctt
      901 cggtcatcac tagcttggcg aggaagaagg cgtccgggtg cgggcacatg ccgtggacga
      961 agatccgctc ggtccaggct gagatatccg agacccgcat cggtcgggcc ggcgtcgggt
     1021 agaagcagac gatgtccaga tccggctgtg tgacgagccg cagatcacgc gagtccgaga
     1081 tcaaggctgc ccaggtcgtc gccgcttgcc gggttcgtgc caggatgcgc cccagtccct
     1141 ccggtgtcag cgggaacagc ttgagcgtcg cccagagcgc ggccgccgcc gcacctgccc
     1201 gagaacattc aaggctgatc tcgccaaggt gcaaatcgca tgaggtgaag taggtgtagg
     1261 gagagtcgtg cttgtagaag cgcccgacgg acgcatcccg gaaaagaacc gatccgcatc
     1321 cgtatggttg caggccatgc ttgtgcgggt cgatcacgat gctgtcagca tcgcccatgg
     1381 ccttgaatgc tgcggcgacg ccgggctcga gcgcctctcc atcggccagg agccggaaaa
     1441 agccgccgta ggccgcatcg acatggacgc ggacgccgtg atcacgacat atcccattgg
     1501 tgatcagaag gtcgtcgacg gcaccgagcg aggtcgtgcc cagcgtcgcc acgacagtgc
     1561 cgatctgccc agcctcgacc gcctcccgca ggtgcgggat cgaaatccgg ccgctcggct
     1621 cggtcgggat cgccacgtgc tcgacaccga tcacgccgca catccgctgg tgcgtgtaat
     1681 gcgcaccggc cgatgacgcg atcgccttgc ccggatgcag ctctcgcgcc acccagagcg
     1741 cctcgaggtt ggcgatcgtg ccggaggagg tgaggtggcc gagactggtc tccggccagt
     1801 acccgaccat cctggccatc gtggcgatca cctcgcgctc catcgccgcc gtcgccggac
     1861 cgccgtcgag cgcgtgattg ttcggattga tctgcatcgc catcgcatat gcggctgccg
     1921 cgaccggatg cggcggcttg agcatctgcc cggcatagac cggatgcggg aagggatagt
     1981 tgtcggtcag ccgctcggcc agcttccgca gcactttgcg gacctcggcc tccgggacat
     2041 cgatcgacgg atcaggcgca aacggtcccc attgccccag ccaatcgctg aggggctggt
     2101 cgccgcgatg gtctgcgctg cgcatcagga gactgcggcc cgcccgtacc cgcgcggcag
     2161 gaatcggccg gatcccgcca gggcaggcaa ccgtacctcg ccctccaacg agtcgtcgaa
     2221 gatcgtctcg ccgcgccgca gcgtccgcac cacccgcccg gtgatggtct tcccctcgaa
     2281 cggggtccac ttctgcctgt gggccatttc ctcgccccgc gccacccacg accgctccgg
     2341 gtcgaagacg acgatatcgg catcggaccc gatcgcgatc gtgcccttgc gcgggtagag
     2401 gtcgaagacc tgggctggat tggtcgcgat ctggcgtacg aactggctca gattcatcga
     2461 atggccgcgc accgcgccgt cgtagaagac cggcagcagg gtctggatcc cggccagccc
     2521 catcggtgcc cggaagatgt cgtcctcacc cgcgtccttc tgctctttcg tgtacccgca
     2581 gtgatcggag cagatcaggt cgaccgtctc gtcaagcacg taggaccaga tgtggtcgac
     2641 ctcgtcctgg gaccgcaggg ccggcgcgca ccggccatac cccttgagcg ccaagaggtc
     2701 gtccgtattc agggacaggt actggggaca ggtctcgacc gtcacgcgca cgccccgggt
     2761 ccgcgcatcg gcgatcagcc gcagggcatc ggcggaggcg caatggcaga tatggaccca
     2821 gcagccggtc acctcggcca ggtagagcgc ctcggccaca gccacggcct cgaccagcgg
     2881 cggacgcgac tccgcatgcg cgatcgggtc agcccgcccg gaatcctgta atcgcttcag
     2941 gccggactgg accaggttgt tgtcctcggc atgcagcgca tagggcatcc ccgtctcggc
     3001 cacgatcgcc atcgcccagt agagtgtcga cgtatcaacc gccgggaacg acgggctcga
     3061 tgacgccatg aacgacttga acgcaactgc tccggtaccg gccagatcct gcagatcgac
     3121 ctcgtcctgc agcggtggac cgacgatgcc ggcccagagc gccatgtcga ctagcgcgtt
     3181 cttgttgacc cgggctcgct tggcctgaaa ctgctcgacc gagatagtgg tcggatccgc
     3241 ctgcggcatc tcgatcacgg tggtgatccc gcccgccgcg gcgccctggc cgccggcgaa
     3301 gaagccctcc ttggtgaact cctccggctc gcggaaatga gtgtggacat cgatcccgcc
     3361 gggcagcacc agcagcccgg tcgcgtcgat gcggtggtca gcgtcaatat cggttgcgtc
     3421 ggtagttagc gcggtgattc gctcgttggc aatcacgaca tctgcgcgga actgtccatc
     3481 gtgcgtgacg acggtaccgc cctcaatcac gacggtatca ctcatgcata ggccttccga
     3541 aatctgtcag gtgagatggg caggcgcctc gtcgggagta tcgtcagccc aatccatcgc
     3601 atgccgaagg ttatagagca tttcggcaaa gcgcagcacc tgaattcggt cggccacggg
     3661 caattcccgc atcaggaagt agagacgcca gtcatccggc gcaaatgcgg agaccgagtt
     3721 ttccggcgga tcgacccgcc cgatctcgaa gtaggttggt ccgcccacgt cgcgcaacgc
     3781 gcgctcggca ggggttacgg tgtttcggga cacggacgcc gcacctgctt cacattcaca
     3841 acccgattcg gggttgccga ctggtcatat ggcccaagtc tactagtcag gcaggatttt
     3901 ggataccggg caaataggta atttgggcta acgacacgag tacgggccgg tgacagttca
     3961 ccggcccgta cgtttaggtg cgcatgtatc gcaaactagc gattgccgtt cggtgggctt
     4021 gtgaaccact cctgcatcgc ctgcttgccc ggacggtcga catggacatg atcgtccacc
     4081 cgggtcaccc aggagagcgg aatccagtga tgttgcccct gctcgtcctt cgtgaccttg
     4141 atcgagtttc cgtcaagatg gtcgaccgtg ccgaattgcc cgccctcgga gcacacaatc
     4201 ggcatatgct ccttgatcat gctggtgtcg acggtcgttt tcatggtctg agtagccata
     4261 acacctacct gttatcgcgg tttgtgacgt ccttcacgac gtcgtccacc ttgtccttcg
     4321 cgtcggccat gccctgcttg gctttaccca tagcctggtc tttctggcct tccatctgcg
     4381 tgctcttgtc gtccgcgagt ttgccaaggg cttccttgcc ctggccctta gcctcgtcga
     4441 acttgccttc aaggcgatcc tgggattgat cagtcatgac cggtcctttc tgtactgccc
     4501 gcatccatca caggtcggtc attcaaccgt ctgcccgaac gcagacaacc atggtaccgg
     4561 cccgtgccaa ggctcccgaa ccgccgaact acgcaactcg ccggatccgg tagttgctgc
     4621 taccgcctcc ctcaggggtg gacgaaaacc ggtcattgca tccatataaa gccggacagt
     4681 cgtaccaatc ccggggtgaa tcgcgggtcg ggaggacaca tgcgcaacgt tgagcaactg
     4741 caaatcgagc tgcgcgaaca cctgcactcg ctcgagcaaa gcggcgtggt cggcctttct
     4801 gcaacgtttc agccggccga atggggggca atcgtgcctc gtctcgacca cgacgagctc
     4861 gttacgctct tcgaatggct gccggaagat gacgcggcca cggtcctggc cgccctggac
     4921 cctcacaccg ccgccgatgt ccttcaggtc attggcccgg tccaggccgc cgccgcgctg
     4981 gacgagatgc ccgaggatga cgcggccgat gtcctcgaag ctctgcccga cgagcacgcc
//
LOCUS       DATAOZ010000194         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6657589, whole genome shotgun sequence.
ACCESSION   DATAOZ010000194 DATAOZ010000000
VERSION     DATAOZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6657589"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VHR64_05225"
     CDS             <1..759
                     /locus_tag="VHR64_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX3302262.1"
                     /translation="DEIEHMQMINSAEELDLLREACKWGDVAHRYLQDFSEVGVSEIE
                     IAQKATMAASQEMLRTLGPEFRPRSIFGSVASAGFRGQVGVASANPHALSNNALLQPG
                     DCLVTGAAASVFGYSGELERSMVVREPSAEQERYFNLMLAAQTLAIETIRPGIPCSAV
                     DDAVNQFFAENGLEETWRHHTGHAKSTLIHEAPFLDRGDHRMIEVGMVFTVEPGIYVE
                     GLGGFRHSDTVAVTETGVEWLTDYPRDLASLIVG"
     gene            1153..1905
                     /locus_tag="VHR64_05230"
     CDS             1153..1905
                     /locus_tag="VHR64_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaA family protein"
                     /protein_id="HEX3302263.1"
                     /translation="MIVLINSSKTMRAVPGSAREQRPALIGRAERLDRALKGMSAAEL
                     KSLMHLSPKLAESTHALIDRWTADSAAQTPAIDAFQGDIFRGLRASTLSADDREYANE
                     VLRILSGLYGILRAFDGITPYRLELMYPLSADGFRNLYEFWGSAVVETLPKDGLIVDA
                     ASDEYARLVRPFVDPDRIVEPQFLTQMSADVEPTFVVVHAKVARGAYARWLIRSRITD
                     PARFGEFADLDWVYDPATSTLDKPVYIKRLAS"
     gene            complement(2147..>3033)
                     /locus_tag="VHR64_05235"
     CDS             complement(2147..>3033)
                     /locus_tag="VHR64_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3302264.1"
                     /translation="VDLFRSGMVDAALVCTPHPAHEPVVVAAADAGVHVLCEKPIATT
                     LGEADRMIAAADRAGIKFGVIFQRRFWPSAQRMRAAIDAGKIGTPILSECAVRLWRGP
                     DYFAMDPWRGKWATEGGGVLMNQAVHSIDHFQWFMGRAVEVSGRIATLRHGGYIDVED
                     TAVATVRFESGALGVIHASSTFQPAYGFRVAVHGDNGATISVWERNEGELGVNDVWSI
                     PGEEALREQWLAEDDGKEGFPDYHVLQIQEFLQAILADRDPAVTGAEARKSLEIILAI
                     YESSRTSLPVPLPMPTAG"
BASE COUNT          494 a          890 c         1096 g          553 t
ORIGIN      
        1 gacgagatcg agcacatgca gatgatcaac tccgccgagg agctggacct gctgcgcgag
       61 gcctgcaagt ggggcgatgt cgcgcaccgg tacctgcagg atttcagcga ggtcggcgtg
      121 agcgagatcg agatcgccca gaaggcgacg atggcggcca gccaggagat gctgcgaacg
      181 ctgggtcccg agttccggcc gcggagcatc ttcggctcgg tcgcgtcggc cgggtttcgg
      241 ggccaggtcg gcgttgcctc ggccaatccg cacgcgctct cgaacaatgc gttgctgcag
      301 ccgggcgatt gcctggtgac cggtgctgcg gcctctgtct tcgggtacag cggcgagctg
      361 gagcggtcga tggtcgtgcg tgagccgagc gcggagcagg agcgctactt caacctgatg
      421 ctggccgcgc agacgctggc gatcgagacg atccgaccgg ggatcccgtg ctcggcggtt
      481 gatgacgcgg tcaatcagtt cttcgccgag aacggcttgg aggagacctg gcggcaccac
      541 accggccacg ccaagtcgac cctgatccac gaggcgccgt tcctggatcg tggcgatcac
      601 cggatgatcg aggtcgggat ggtcttcacg gtcgagccgg gaatctacgt cgagggcctg
      661 ggcggcttcc gccactcaga cacggtcgcc gtcaccgaga ccggcgtcga gtggctgacg
      721 gattacccgc gggacttggc gagcttgatc gtaggctagg cgagcagtcg ggaggcgggc
      781 aggcgagtag cttggcgatc agggatcgca gaactgacag cccgtaactg tatggcccgc
      841 gcgttcgccc cagtcaaaca agtcgtctct cgagttggaa catgcttttg gatacgtgag
      901 gtggtcaacc gattcgtcgg gcgggtatag gctcttgcaa cgggcggtgt ggagaattga
      961 ctggtcttta ccgcttgtgt ggagattcag aacgaacccg gcgggattgc gatagagcca
     1021 ggagatgtac tcccggctgg ttttcggttc cagaaccatt ggtcgttcgc cctcgatgtg
     1081 cccggacagg aatagtcttg ccacaatgag gaatataagc tgccgtgcag atcaaatagg
     1141 tgaaagcggt cgatgattgt gctcatcaat tcgtcgaaga cgatgcgggc ggtgccgggg
     1201 agtgcgcggg agcagcgacc ggcgctgatc gggcgggcgg agcggctgga tcgggcgctg
     1261 aaggggatgt cggcggcgga gttgaagtcg ctgatgcacc tttcgccgaa actggcggag
     1321 tcaacacacg cgctaatcga ccggtggacg gctgactcgg ctgcccagac gccggcgatc
     1381 gatgcctttc agggcgacat cttccggggt ctgcgcgcaa gtactctgtc ggccgatgat
     1441 cgcgagtatg cgaacgaggt gctgcggatc ctctccgggt tgtacggaat cctgcgggcg
     1501 ttcgacggga tcacgcctta ccggctggag ttgatgtacc cgctctcggc cgacggattc
     1561 cggaatctct acgagttctg gggatcggca gtggtggaga cgctgcccaa ggacggcttg
     1621 atcgtggacg cggcatcgga cgagtacgcc cggctggtcc gccccttcgt cgatccggac
     1681 cgcatcgtcg agccgcagtt cctgacgcag atgtcggccg atgtcgagcc gacgttcgtg
     1741 gtggtccacg ccaaggtcgc gcgcggcgcg tatgcgcggt ggctgatccg atcccggatc
     1801 acggacccgg ctcgcttcgg cgagttcgcg gacctcgatt gggtctacga tccggcgacg
     1861 agcacgctgg acaagccggt ctacatcaag cgattggcga gttgattgat gccgtattgc
     1921 tgatgaaggc gtagcgcggc ccgcctctgg gccgcatcgg tatcgggcgg gttggttgat
     1981 gacgcaggtt tgctccatca atcgtgcgta ccgtcagcca atccggccac ggggggccgg
     2041 cgctacgcgg atgtgtcgtt accgagccgg tcgcaggggt tggcgttacg ccgttgtgcg
     2101 gtggccatgc cggacgcggg ggtcggcgct gagcctgggc tgcggatcag ccggcggtgg
     2161 gcattggcag gggaacgggg aggctggtgc ggctggattc gtagatggcg aggatgatct
     2221 cgagcgactt gcgggcttcg gcgccggtga cggctgggtc gcgatcggcc aggattgcct
     2281 gcaggaactc ctggatctgg aggacgtggt agtcggggaa gccctccttg ccgtcatcct
     2341 cggccagcca ttgctcgcgt agcgcctctt cgccggggat gctccagaca tcgttgacgc
     2401 cgagctcgcc ctcgttgcgc tcccagacgc tgatcgtcgc gccgttatcc ccgtgtacgg
     2461 ccacccggaa gccatacgcc ggctggaagg tcgagctggc gtggatgacg ccgagtgcgc
     2521 cgctctcgaa ccggacggtc gcaaccgctg tgtcctcgac atcgatgtat ccgccgtgcc
     2581 ggagcgtggc gatgcgaccg ctgacctcga ccgcgcggcc catgaaccac tggaagtggt
     2641 cgatcgagtg gaccgcctgg ttcatgagga cgccgccgcc ttcggtcgcc catttgccgc
     2701 gccagggatc catcgcgaag taatcgggtc cgcgccagag gcggacggcg cactcgctga
     2761 gaatcggcgt gccgatcttg ccggcgtcga tcgcggccct catacgctgg gccgatggcc
     2821 agaatcggcg ctggaagatg acgccgaact tgatcccggc ccggtcggcg gcggcgatca
     2881 tgcggtccgc ctcgccgagg gtggtcgcga tcggtttctc gcagaggacg tggacgccgg
     2941 cgtcggcggc tgccacgacg accggctcat gggcggggtg tggcgtgcag accagcgcgg
     3001 catcgaccat gccgctgcgg aacaggtcga cca
//
LOCUS       DATAOZ010000195         6240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_606699, whole genome shotgun sequence.
ACCESSION   DATAOZ010000195 DATAOZ010000000
VERSION     DATAOZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606699"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1893
                     /locus_tag="VHR64_05240"
     CDS             205..1893
                     /locus_tag="VHR64_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302265.1"
                     /translation="MPGVLEGEIEPAELTVEEEHLLVLLTKADDRFQEIVERQSKEGL
                     ATVLGALDDAGITLPEPGIALQDLERMQHVWVQYANGPEWIDLDPSIPNAERGTVYAE
                     TTESRDQLPEEFFHRISMRLVAEKVVGGAPKRGDLLAFEATSAELVGVPITLMHPNAD
                     SLKAAGVAIQNVIGGYRNFIPSLIVGNQSQAGQPVTFVTGEGALGAFDVTSIEGDTLG
                     EWLEIEVRTPGGSRQITRELFDRVGLARRGAGSIDLGAISPVELVDTGSAAPGYPPLN
                     SALSIGIVSGIVPWRYFEADEETQDPLVALSRAVHVYHYIRDVLALESAELNGQRFSI
                     DEPNVTAVLQAEFTSTADEQISTLLMDLIHRHFVAVPVEGVETRMQSQVAAGIFGHAV
                     ERALIETAGAELLGLPIDDFMSVGRVFEEAQQQDIPVRVLTPGASNAAPLSLDEAARL
                     RIEAALADGYTVIVPERSVAVGNTVRSGWWLVDPKTGATQDQLDDGRGTTLVEYAFTI
                     ARIMICAAAAFHALGLVSLIHTYGAGNQLQEQWIKNRVKKLSISGGLCLGSFAV"
     gene            complement(1919..>6240)
                     /locus_tag="VHR64_05245"
     CDS             complement(1919..>6240)
                     /locus_tag="VHR64_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3302266.1"
                     /translation="LANILLPRGEYLVRITGDPDPANPYILRLDVTTPPANNFESEPN
                     DSEALAAPLDPTVPLTGRYDGNETDVYQFTTTGEPQLWDVEVSGTRISQLDFTRRDGT
                     ELSTAEVSADQTTARLSDLYLTPGDHWLRTSGENGDYTITLTPLGAPLPDGEHETNNL
                     VTTAEPMLVGDTKTGRLVESTDVDVFRFSLAAQEHITLALDAPTDGSLKIRVDWGNAL
                     VASDVSTAPGDKTRLDLLLLPGDYVVTLWPSQVSTNRYAFKIVRDDPFLLSDDQEPND
                     TISTAQRLPAGKEMQGALSGDDAHDIYLIPPGSGRDLTFTLESDGVYPAQATDGQNVY
                     SLTPSADGKSWTLDQAVPDDVPLYFDLTGSGTYRLTYGAVQSPAGPKLPTILGGGEKA
                     PLQVSLALDATEVAAYLTEGQIVDGTLTITNPTTASVDFTLLLATSHFHVTVEIDDAP
                     SSIAAGQTLTVPISVQIEPDAWANIPVRITIGLEIGDLTASAFAEITPTAESPPINPV
                     QAWPIPYDLLGGLDVASPELGATVAGTVNPDGEALLHDGVAPVGSGLSLSSPALPLTL
                     TVDLAGVAPVPVRGIILNPIGLDGVPQEQVRRFELQLSPDGVNYTSALTGGLSAVPRE
                     QAFLLPQVMEATHARLIILSTYDSNPTQPSYLGLGEWNVIAEPGYVPETVDRLSIADP
                     AFGGHIVRTDPQLGNPDFWTSLLDADTTNRYTNVSESGEISWVIGFEDNRAAQITDLA
                     WIDPDGTDPNQRIASVTVEVTTGDPSGPWQSLGTWTLKRNSSGGVRRFKLDAPTWARF
                     VRISTTIAPNTGVEYPGQIQIYEAPIGDSYRSVIGAWGGNSAAAWYEMQNPPATQLVQ
                     DETDAPDQQERAAPLTLGQTINGRAHGGTDVDWYRVTAPQDDNTLSFTLTGVPTVDVV
                     ARVYDASGTEAPVTRTVGGDTSTIVYTASVTAGAEYTVKIEQPPHSIVFAFDTSTSMG
                     LYEPMVQQSLRSFAGGIEKGQEFVQVLPFDEDPLLENWTDDAYLVYSAINGYYDKSLS
                     SSAEATILDATKLLSGQEGQTAILIITDAETSSFAQGTEMWQQLDVVQPRIFAVHVGG
                     SSTPVLNEHLMEDWSSSGGYYQYTLNQSEMDRAFDRAATWLRRPAGYSLTVSSSFAEP
                     ATPEASPTAEPTSTPEPTATEVSTEPGTLAVVAPTSGSEAPSAQVSDQVTIEIILDTS
                     GSMLAAMPDGQRRIDVARNVLSDLVTNQLPAGIPVTLRTFGYQPDSCDTQQIVPVQPL
                     DPAAMSATIQGIEPVNLVRTPIGASLEQVANDLAGVEGPKVVVLVTDGEETCNGDPAA
                     AIQSLIDQGIDVQVNIVGFALDDDALRAQVRDWARIGNGSYFDATNAQELDQAIAKAV
                     QAPFRVLDAGGNEVARGTVGGDPIDVPPGAYTVVVLTDPEQRFENVVIEPGGQIELQL
                     SGP"
BASE COUNT         1084 a         1913 c         2051 g         1192 t
ORIGIN      
        1 gtcttgcggg gagccaaagg aacgctctgg ggattggcgg gaaactcggc cgatcaggca
       61 atgcttcttg ctgcgctcct gaatgcctcg atgatccaaa cgcgattcgc cttcggcaaa
      121 ctcccggacg atgccgccga gaagttgttg gcgtcgctac gactcgacga gggcacggtc
      181 cgcgcccggg cggatcgggt cctcatgcct ggggtacttg agggagagat cgaaccggcc
      241 gagttgacag tcgaagagga gcatctgctt gtcctgctga caaaggcaga cgatcgattc
      301 caggagatcg tcgaacgcca gtcaaaagag ggtcttgcca cggtactcgg cgcgttggat
      361 gacgctggta tcaccctacc ggaacccggc atagcccttc aggacctcga acgcatgcag
      421 cacgtctggg tgcagtatgc caacgggcca gagtggatcg atctcgaccc cagcatcccg
      481 aacgccgaac ggggaactgt ttacgctgaa acgaccgaat ctcgcgacca gctaccggaa
      541 gagttcttcc atcgcatctc catgcggctg gtggcggaga aggtggtggg cggagcgcca
      601 aagcgcgggg atctcctggc attcgaggca acgagcgcgg agcttgtcgg cgttccgatt
      661 acgttgatgc atcccaacgc ggactcgctc aaagcggcgg gcgtagcgat tcaaaacgtc
      721 atcggagggt atcgcaattt catcccatcc ctcatcgtcg gtaatcaatc ccaggccggc
      781 cagccagtca cgttcgtcac gggggagggg gcgcttgggg cattcgacgt gacttcgatc
      841 gaaggggaca cgctcggaga atggcttgag atcgaagtta gaacgcccgg cgggagccga
      901 cagattactc gcgagctttt cgaccgggtc ggtctggcgc gccgtggcgc cgggtccatt
      961 gatctcggcg cgatttcccc cgttgagctt gtcgacactg gcagtgcagc tcccggctat
     1021 cctccgctga actccgcctt gtcaatcgga atcgtcagcg gaattgtccc ttggcgctac
     1081 ttcgaagcgg atgaggaaac tcaggatccg cttgtcgccc tgagccgtgc tgttcatgtg
     1141 tatcactata ttcgcgacgt cctggctctg gagagcgccg agctgaacgg ccagcggttc
     1201 tccatcgacg aacctaacgt gactgccgtc ctgcaggccg agttcacgtc taccgccgat
     1261 gagcaaatct ccaccctgct gatggacctg atccaccgcc atttcgtcgc tgtcccggtt
     1321 gagggcgtcg aaacccgaat gcaatcacaa gtggccgcgg gcatctttgg ccacgccgtc
     1381 gaacgtgccc tgatcgaaac cgccggggca gagctgctgg gcctgccgat cgacgacttc
     1441 atgagcgtcg gccgagtgtt tgaggaggcg cagcagcaag acattccggt tcgcgtacta
     1501 acccctggcg ccagtaatgc cgcgccactt tccctggatg aggcagccag attgcgcatt
     1561 gaggctgctc tggcagacgg gtacacggtg atcgtccccg agcgatccgt cgcagtcggc
     1621 aacactgtgc gctcaggctg gtggctagtc gatccgaaga ccggagccac gcaagaccaa
     1681 cttgacgacg gcaggggtac cacgcttgtc gaatatgcgt tcacaatcgc cagaatcatg
     1741 atctgcgcgg ccgccgcttt tcatgctctt ggcctggtgt cgctcatcca tacctatgga
     1801 gcagggaacc agttgcagga gcaatggatc aaaaatcgag tgaagaagct ctcaatttcc
     1861 ggcggcctgt gcctcggatc gttcgccgta tagatcacgt ctgcatacag caggcaaact
     1921 agggtcctga gagctgcaat tcgatctggc cgcccggctc gatgacgacg ttctcgaagc
     1981 gttgctctgg atcggtcagc acgacgaccg tgtaggcgcc cggcgggaca tcgatcggat
     2041 cgccgccgac ggttccacgt gcgacttcat tgccgcccgc atccaacacc ctgaagggcg
     2101 cctggaccgc cttggcgatc gcctgatcga gctcctgcgc gttggtggcg tcgaaatatg
     2161 aaccgttgcc gatccgcgcc cagtcccgga cttgcgcgcg tagggcatcg tcgtcgagcg
     2221 cgaagccgac gatgttgacc tggacatcga tgccctgatc gatcagcgac tggatcgccg
     2281 ccgccgggtc gccgttgcag gtctcctcgc cgtccgtgac gagcacgacg accttcggac
     2341 cctcgacgcc ggccagatcg ttcgcgacct gctcgagcga ggcgccgatc ggcgttcgga
     2401 caagattgac cggctcgatg ccctggatcg tggccgacat cgcggccgga tcgagcggct
     2461 gaaccggcac gatctgctgc gtgtcgcacg aatctggttg atatccgaag gtgcggagtg
     2521 tgaccgggat ccccgccggc aactggttgg tgaccagatc gctgagcaca ttgcgggcga
     2581 cgtcgatgcg gcgctggccg tccggcatcg ccgcgagcat cgatccggac gtgtcgagga
     2641 tgatctcgat cgtgacctgg tccgagacct gcgcgctcgg cgcctcgctg cctgaggttg
     2701 gcgcgactac cgcgagcgtg cccggttcgg tcgagacttc agtggccgtc ggctcgggtg
     2761 tgctggtcgg ctcggcggtc ggcgatgcct ccggcgtggc cggttccgcg aacgacgagg
     2821 acacagtcag gctgtaaccg gctggtcgac ggagccaggt tgcggcgcgg tcgaaggcgc
     2881 ggtccatctc cgactggttc aacgtgtact ggtagtagcc gccgctgctc gaccagtcct
     2941 ccatgaggtg ctcgttgagc accggtgtgc tcgatccgcc gacgtggacc gcgaagatgc
     3001 gcggctggac gacgtcaagc tgttgccaca tctccgtgcc ctgggcgaag ctgctggttt
     3061 cggcatcggt gatgatcagg atcgcggtct ggccctcttg accagacaat agcttggtcg
     3121 cgtcgaggat cgtcgcctcg gccgagctcg agaggctctt gtcgtagtac ccgttgatcg
     3181 ccgagtagac cagataggca tcgtcggtcc agttctcgag cagcgggtcc tcgtcgaacg
     3241 gcaggacctg gacgaattcc tgccccttct cgatgcctcc ggcgaagctc cgcagcgact
     3301 gctgaaccat cggctcgtac aaccccatgc tggtggatgt gtcgaaggca aagacgatcg
     3361 agtgcggtgg ctgctcgatt ttgacggtgt actccgcgcc ggccgtgaca gacgccgtgt
     3421 agacgatggt cgaggtatca ccaccgaccg tgcgggtcac cggagcctcg gttccggacg
     3481 catcgtagac ccgcgcgacg acgtcaaccg tcggtacgcc ggtcagcgtg aagctgagcg
     3541 tgttgtcatc ttgcggagcg gtcacccggt accagtcgac atcggtgccg ccgtgagcgc
     3601 ggccgttgat cgtttggccg agggtcagcg gcgcggcccg ttcctgctga tcgggcgcgt
     3661 cggtctcgtc ctgaacgagc tgggtcgccg gcgggttctg catctcgtac caggctgccg
     3721 cgctgttgcc gccccacgcc ccgatcaccg agcggtagct gtcaccgatt ggcgcctcgt
     3781 agatctggat ctggcccggg tattcgacgc cggtattcgg tgcgatggtg gtcgagatgc
     3841 gcacgaagcg cgcccacgtc ggcgcatcga gcttgaaccg ccgcactccc ccactgctgt
     3901 tccgcttcaa ggtccaggtg ccgagcgact gccatggtcc cgacgggtcg ccggtggtta
     3961 cttcgaccgt caccgacgcg atgcgctgat tcgggtccgt tccgtccgga tcgatccacg
     4021 caagatcggt gatctgtgcg gcgcggttgt cctcgaaccc gatcacccag ctgatttcgc
     4081 cactctcaga gacattggtg taccggttcg tcgtgtcggc atccagcaac gatgtccaga
     4141 aatccggatt gccgagttgc ggatcggtcc gcacgatgtg tccaccgaat gcgggatcgg
     4201 caatgctgag gcggtcgacc gtctccggca catagcccgg ttcagcgatc acgttccact
     4261 cgccgagacc gaggtaggat ggctgggtcg ggttgctgtc gtaggtgctg agaataatga
     4321 gccgagcgtg cgtcgcctcc atcacctgcg gcagcaaaaa tgcctgctcc cgcgggaccg
     4381 ccgagagccc gccggtcaag gcagatgtgt agttcacgcc gtcaggcgag agctggagct
     4441 cgaatctgcg gacctgctcc tggggcacgc cgtctaagcc aatcgggttg aggatgatgc
     4501 cgcgaaccgg caccggcgca acgccggcca gatcgacagt cagcgtcagc ggcaatgccg
     4561 ggctgctcag cgacagaccg gaaccgaccg gcgcaacgcc gtcatggagc agcgcttcgc
     4621 catcgggatt aacggtgccg gccacagtcg cgccgagctc cggggacgcc acgtcgagcc
     4681 cgccgagcag gtcgtagggg attggccagg cctggaccgg gttgatcggc ggcgactcgg
     4741 cggtcggcgt gatctctgcg aaggcggagg cggtaaggtc gccgatctcg agcccgatcg
     4801 tgatccggac cgggatattc gcccatgcgt ccggctcaat ctgaacggag atcgggacgg
     4861 tcagcgtctg ccctgctgcg attgacgacg gggcgtcgtc gatctcgaca gtgacgtgga
     4921 agtgcgacgt cgcgagcagc agggtgaagt cgaccgacgc agtggtcggg ttggtgatcg
     4981 tcaacgtgcc gtcgacgatt tgcccttccg tcaggtaggc ggccacctcg gtcgcgtcca
     5041 gcgctagcga gacttgaagc ggggctttct cgccgccacc gaggatcgtc gggagcttcg
     5101 gtccggccgg actttggaca gccccatagg tcaaccgata cgttcccgac ccggtcaggt
     5161 cgaaatacag cgggacgtcg tccggcaccg cctgatcaag agtccagctc ttgccgtcgg
     5221 cggacggtgt cagtgagtag acgttctggc cgtccgtcgc ctgggccgga tagacgccat
     5281 ccgactcgag tgtgaaggtc agatctcgcc cacttcccgg cgggatcagg tagatgtcgt
     5341 gggcatcatc accgctcagc gcgccctgca tctccttgcc ggcgggcaag cgctgggcgg
     5401 tcgagatcgt gtcgtttggc tcctgatcgt cgctgagcag gaacgggtcg tcgcgcacga
     5461 tcttgaaggc gtaccggttt gtgctgacct gactcggcca caacgtgaca acgtaatccc
     5521 ctgggagcaa caggaggtcg agcctggtct tgtctcccgg cgccgtcgat acatctgatg
     5581 cgacaagcgc gttgccccaa tccacccgta tcttgaggct accgtcggtc ggagcgtcca
     5641 gtgcaagcgt gatgtgctcc tgggctgcca acgagaagcg gaagacgtcg acatcggtgg
     5701 actcgacgag acggccggtt ttcgtatccc ccaccagcat cggctcggcg gtggtgacca
     5761 ggttattcgt ctcgtgctcg ccgtcgggca acggcgcacc gagcggcgtc agtgtaatcg
     5821 tgtaatcccc gttctcgccg cttgtccgca gccaatgatc gccgggcgtg aggtagaggt
     5881 cggaaagccg cgcggtggtc tgatcggcac tgacttcggc cgtcgagagc tcggtgccat
     5941 cacggcgggt gaaatcgagc tggctgattc gggttccact gacctcgaca tcccagagct
     6001 gcggctcacc ggtcgtcgtg aactggtaga catcggtctc gttgccgtcg tagcgtccgg
     6061 ttagcgggac cgttggatcg agtggcgcgg cgagtgcttc ggagtcgttc ggctcgctct
     6121 cgaaattatt ggccggcggc gtggtcacat cgaggcggag gatgtacggg ttggccgggt
     6181 cgggatcgcc ggtgatgcgg accagatact caccgcgcgg aaggaggata ttcgccagcg
//
LOCUS       DATAOZ010000196        10428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4555693, whole genome shotgun sequence.
ACCESSION   DATAOZ010000196 DATAOZ010000000
VERSION     DATAOZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10428
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4555693"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VHR64_05250"
     CDS             complement(<1..1461)
                     /locus_tag="VHR64_05250"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX3302267.1"
                     /translation="MPQYIFVTGGVVSSVGKGITTASIGRMIKARGMSVSIMKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSRASNVTTGQIYSAVIQKE
                     RRGDYLGGTVQVIPHVTNEIKHRIMLASKQHNSEVVICEVGGTIGDIEGQAFIEAIRQ
                     MRRDAGRQNVMYIHVTLVPILGSNGEIKTKPTQQSVRELRGAGIQPDVIVCRSDGELS
                     DDVRDKIALFCDVDKEAVIPLPTAETIYEVPLILEEFGLGEYIARHFGFAAKAPDFTE
                     WRSLVERIKTPRRTIRVALVGKYVDLHDSYMSIAESLTHAGLHYDTEVNIHWLNSETL
                     TPEELERELKKVSGVVVGPGFGPRGTEGKVATARYARESGLPYFGICYGLHMAAIEFA
                     RNVLGMTGANSTEIDAETPYPVIDLMPTQKGVEMGGTMRLGLWPCWLQEGTKAAEAYG
                     ESHISERHRHRFEVNNDFREQMAKAGFVASGTSKDGN"
     gene            complement(1577..2233)
                     /locus_tag="VHR64_05255"
     CDS             complement(1577..2233)
                     /locus_tag="VHR64_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3302268.1"
                     /translation="MAEIRWFGHNCFRIRAKEATVIIDPVGKNTGYAMARQTADIVLL
                     SHDHPGHANMNGVKPGYQIVEGPGEYEMHDVFITGIRTYHDAEKGAQNGYNTAYLIDL
                     EGMKFCHLGDLGHMLNEDQLEVLNSCDILMIPVGGGSVITTEQAAELIGQLEPKVVLP
                     MQYATAIGDKKLATLEPFTKALGVTVPDAEEKLTLRSSELSETMRLVVLSPEAEAARK
                     "
     gene            complement(2560..2898)
                     /locus_tag="VHR64_05260"
     CDS             complement(2560..2898)
                     /locus_tag="VHR64_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3302269.1"
                     /translation="MQRYLISFDDGWMTFPEEDLPAVAEAAHKVVHEAKDAGVWIFGG
                     GLRTQSASIVSTDGTITAGPNPETKAVIGGFSIIEVPSREEALEWATRIAAACRCTQE
                     VREIMYDPES"
     gene            complement(3222..3896)
                     /locus_tag="VHR64_05265"
     CDS             complement(3222..3896)
                     /locus_tag="VHR64_05265"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX3302270.1"
                     /translation="MNVNGWYKGVRATARRMGMRRATEIAKGHEHWRSEWISLGLRRD
                     LQVPFTAPEAKIPLIVRPLQDEDWPFLFDLTEPGISDEERQLRTSRKELALERVGSPF
                     VAATADGEPCYIQWLFSPDENERMREFFNNIFPRLTPDEMLLEGAFTPEKHRGKGIMP
                     CAMAQIAEHAAARGARWVVTFVTEDNVPSLKGCDRAGFSPYIRRSVRWNGLRRTVTFG
                     ELGIES"
     gene            complement(4081..4755)
                     /locus_tag="VHR64_05270"
     CDS             complement(4081..4755)
                     /locus_tag="VHR64_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX3302271.1"
                     /translation="MRRQKSPLVRPPRAAEAGKIASVTSAGRGGRRVEITMESGTTYT
                     VDSNVAYDAGIAPNLTLEQAEIQALLDSDDLLAAKQVATRQLSYRPRSISELRQTLGQ
                     RGFGPQIIDQVIERFSELGYLDDADFARRWIQTREQLAPRGTRLLKQELRQKGIDTDL
                     ADEALADADLDELDAATRIAERRLPKMTGLDRDTQRRRLAGYLERRGFSHDVVRQIDR
                     KYFREM"
     gene            complement(4760..5335)
                     /locus_tag="VHR64_05275"
     CDS             complement(4760..5335)
                     /locus_tag="VHR64_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX3302272.1"
                     /translation="MAALNPTIVKAIKANGLLKEGHFAYRSGQHSHWLIDRDQLLTEP
                     EFASHLGYAIAKRFFTDKIETVATPSIWGAGVAQWIAYFLEPRARIVHATPIDGEPTV
                     APILEGLIRDKRLLLCDNLIMSGVTMSKFTDVIEGLGGRIVGIATIWNSQGDEVGGHP
                     VFGLLNTLYEPRHPDDCELCARGVPVEHIPY"
     gene            complement(5417..5725)
                     /locus_tag="VHR64_05280"
     CDS             complement(5417..5725)
                     /locus_tag="VHR64_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302273.1"
                     /translation="MHVSDWNDLMQFVWTLPSLEEMSAPPILEFSGRYQDMLRAVGGW
                     LDARGYRLVGLSGTGDSLLVEVEAGPPGTDICREAFRLDFGSLERLATAAQADRDRFQ
                     "
     gene            complement(5886..6923)
                     /locus_tag="VHR64_05285"
     CDS             complement(5886..6923)
                     /locus_tag="VHR64_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX3302274.1"
                     /translation="MAAPLNENHWAERCLANMRQTVSVLAERAPLLAEMAEYHLGWTG
                     DLAGQDRYRGKMIRPMIAIFAARAVGGDTAGDAAVPLAAAIELLHNFTLIHDDIQDNS
                     PARRHRPTVWARWGEAQAINAGDALFAAAHLALLSATESGVAPSVVIDLIRGFDATTI
                     EIVSGQVLDLQFEQRQDVTSAEYIEMITGKTARIVEFAAGGGAWIGGAGPELSAHLAE
                     FGLALGLGFQVQDDLLGTFGAAEDTGKAAADDIRRKKQSLPVLLLRQLAQPEDRARLA
                     EMYDRPEIDPAAVAEILDLMDRYQIEARVRETVTGYHDQAVAALTATGLDEQALAPLM
                     DLTRRLESRTA"
     gene            complement(6945..7937)
                     /locus_tag="VHR64_05290"
     CDS             complement(6945..7937)
                     /locus_tag="VHR64_05290"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX3302275.1"
                     /translation="MQSVLANVTRGDHIESIHHGSIAVVDSSGALVAKVGDPDLFLYF
                     RSSAKPFQAIPVIASGAADRFGFTPAELALCCASHSGTPEHQRQVWAMLAKLGLDETA
                     LQCGSPSPYDETAFSQVEAGNVPRSPTQCDCSGKHSGMLAACLQLGYQIDNYLDPCHP
                     LQQTILGLIAEVCGVERDTIALATDGCSLPTFGSTLRAFACSYAVLARPSIHADALTR
                     LRDAMMDVPENVGGPGQFVTDLMAAGNGTIVAKSGAEGLICIGIPAHGLGIAIRIADG
                     SFRAHPVVAAEVLRQLNVVPESVIDDVLKLHDPTIYNHNRRIVGEIRPAFTLDA"
     gene            8099..9652
                     /locus_tag="VHR64_05295"
     CDS             8099..9652
                     /locus_tag="VHR64_05295"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX3302276.1"
                     /translation="MTRFSRRTLLAGAGVLPILGTGRAAKAQTESPITPIEPSETPTD
                     NGTATVSGRYFGDTGHNLKAPFLRAWQAAGGRDGIGAPISEERFQEHVGVTQSFQAVS
                     LVFDPGLESPWDIQATHIPDDVRRAIAPGNARNQVSGASNNGRYFQETGHSLSGKFLK
                     FWNEHGDLPLIGMPTSEPFRDAQTSAQRQVFERAVLELGQDGVVRFLPVAAQIAEDSG
                     LASDPAFLPNPPVAGKTQLVKSPEGLRLRARPNLSADVIVVLPDNAEFIAAADETGDW
                     YAGYADGFAGYVASEFLTEAPPLPEISLADWDTSVWQGAALGETNLRADPTTRSDIDR
                     VLEYGDEVTVTDWVKGEEVFVGADLWARLKDGGYIYARNVGRNAPVAPTPVPADAPTV
                     GRWIDVNLTQQLMVAYEGRNVARVIVTTTGMAGWETPPGFYQILWRVPNETMESGAIG
                     AEHFFKLEDVLFTQYFTNQGHAIHYAWWRTPQTIGRPGSHGCLNTLLDDAQYLWDWAT
                     IGTPVYVHK"
     gene            9769..>10428
                     /locus_tag="VHR64_05300"
     CDS             9769..>10428
                     /locus_tag="VHR64_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302277.1"
                     /translation="MIRVGMRVAVLGLLVAALLGLVTGSVAAQTAKAVDETSPTPGQN
                     ITYTITIDESADQHNGRAIRSVTITDQLSAGFTFVSVTCSLSCTATTPAVGSEGTVTI
                     TGFGQTEGPFDPTMTGTVTLVVAASDTVGATFTNQACIDVFVTPTISDPQFCVSAPAV
                     TVIDAPTATPTETSTATPTATNTPTATNTPTVTATSTASATPTNTVQPTATSTASPTS
                     TA"
BASE COUNT         1844 a         3268 c         3395 g         1921 t
ORIGIN      
        1 attgccgtcc ttcgaggtgc cgctggcgac gaatccagcc ttggccatct gctcccggaa
       61 gtcattgttg acctcgaacc tgtggcggtg ccgctcggag atgtgcgact cgccgtacgc
      121 ctcggctgcc ttcgtgccct cctggagcca gcagggccag agaccgagtc gcatcgtccc
      181 gcccatctcg acacccttct gcgtcggcat caggtcgatg actggatatg gggtctcggc
      241 gtcgatctcg gtcgaattgg ccccggtcat gccgaggaca ttgcgcgcga actcgatcgc
      301 cgccatgtgc aggccgtagc agatcccgaa gtacggcagg cccgactcgc gggcgtaccg
      361 ggcggttgcg accttgcctt cggtgccgcg tggtccaaat ccaggcccga ccacgacgcc
      421 cgagaccttc ttcagctcgc gctccagctc ctcgggcgtc agcgtctcgg agttgagcca
      481 gtggatattg acctccgtgt cgtagtggag gccggcatgc gttagcgact cggcgatgct
      541 catgtaggag tcgtgcagat cgacgtactt gccgaccagc gcgacccgga tcgttcggcg
      601 cggggtcttt attcgctcca ccagcgatcg ccactcagtg aaatcgggcg ccttggcggc
      661 gaagccaaag tggcgcgcga tgtactcgcc gaggccgaac tcctcgagga tcaacggaac
      721 ctcgtagatc gtctcggcag tcggcagcgg gatcaccgcc tccttgtcga catcgcagaa
      781 gagggcgatc ttgtcgcgga cgtcgtccga cagctcgccg tccgaccggc agacgatcac
      841 gtccggctgg atacccgcgc cgcgaagctc ccgcacgctc tgctgggttg gcttggtctt
      901 gatctcgccg ttgctgccga ggatcgggac cagcgtgacg tggatgtaca tcacgttctg
      961 gcgaccggcg tcgcggcgca tctggcggat cgcctcgata aacgcctgac cctcgatgtc
     1021 gccgatcgtg ccgccgacct cgcagatcac gacctcggag ttgtgctgct tcgaggcaag
     1081 catgatccgg tgcttgatct cattggtgac atgcggaatg acctgaaccg tgccgcccag
     1141 gtagtcgccg cgccgctcct tctggatcac ggccgagtaa atctggccgg tggtcacatt
     1201 cgaggcgcgg gagaggttct cgtcgatgaa gcgctcgtag tgaccgaggt cgaggtcggt
     1261 ctcggcgccg tcatcggtca cgaacacctc gccgtgctgg tagggcgaca tcgtgccggg
     1321 gtcgacgttc aggtacggat cgagtttcat gatcgaaacg ctcatcccgc gggccttgat
     1381 catgcgcccg atcgacgcgg tggtaatgcc tttgccaact gaagagacga caccgccggt
     1441 tacaaagatg tactgtggca tgttcccctc cgcatcccct tattccacat aattcgggca
     1501 aaaagagggg aggcggcgct gctgcgccgc ctcccggtgc atccatttcc tgtactggcg
     1561 cggggctgac cctggatcat ttgcgcgctg cctcggcctc tggcgaaaga acgaccaaac
     1621 gcatggtttc ggacaattcc gagctgcgaa gcgtcagctt ctcttcggcg tccggaacgg
     1681 tcacgcccag cgccttggta aagggctcga gcgtggccag ctttttatcg ccgatcgcgg
     1741 tcgcgtactg catgggcagg actaccttcg gctcaagttg gccgatcagc tcggccgcct
     1801 gctcggtcgt gatgaccgat ccgccgccga ccgggatcat caggatgtcg cagctgttga
     1861 ggacctcgag ttggtcctca ttgagcatgt gaccgagatc gccgaggtgg caaaacttca
     1921 tgccctcgag gtcgatcaga taggcggtgt tgtacccgtt ctgcgcgccc ttttcggcgt
     1981 cgtgataggt gcggatgccc gtgatgaaga catcgtgcat ctcgtattcg cccggaccct
     2041 cgacgatctg gtagcccggc ttgacgccgt tcatattggc gtggccgggg tgatcgtgcg
     2101 agagcaggac gatgtctgcc gtttgcctgg ccatcgcgta tccggtgttc ttgccgacgg
     2161 ggtcgatgat gaccgtcgcc tctttggcgc gaatccggaa gcagttatgg ccgaaccatc
     2221 gtatctctgc catatccctc aaaccggccg aaaacggacc gggtcctggt aaacggaaaa
     2281 cgacaagccg gccgttcgct cgtggaacga cgcgcttggt ggaggggcaa aacaacgccc
     2341 accgcatgtg cggatgtttg gggtgcctct ctcagtatat cacgggtgga gggttaagag
     2401 ttgagggttt agggttaagg gagacaaggt gagcagccgg aaaaccgaaa acggaaaact
     2461 gtccgccagc gccttttctg ttcccttaac ccccaactct taacccttaa ctcgctgaac
     2521 ctgaccgttt tcctaaggcc atactgttcg ggccggcggt caggattccg ggtcgtacat
     2581 gatctcgcgg acttcctggg tgcaccggca ggcggcagcg atcctggtgg cccactccag
     2641 cgcctcctcg cgggagggca cctcgatgat cgagaatccg ccgatgactg ccttggtctc
     2701 cgggttgggg ccggcagtga tcgtgccgtc ggtagagacg atgcttgcgc tttgggttct
     2761 caaaccgccg ccgaaaatcc acacgccggc gtccttggcc tcatggacca ccttgtgcgc
     2821 ggcctcggcc acggccggga ggtcctcttc ggggaaggtc atccatccgt catcgaacga
     2881 gatcaggtac cgctgcattc cgtaactcct cgtcccagcc tctgtggccg gctcttccat
     2941 tagtcgaact ccgcgccccg gattcgacaa gtgaccagtc gtggcggcgc gggagccata
     3001 actccttaac ccaacgcacg taccacgaaa ggatggcggg tgacggagtg gatggggtac
     3061 gcgagacatg gattgcgggc gaagcgccat ggggagtgct tgcaaaggtc ctgcagccgg
     3121 agtttgtggc gcggaggcgc gaagcaggag aacggaatac gggaaacggg aaaccaaaga
     3181 tgagagacca aaggccggtt gctttaccgt tcccgtaacc cttaactctc aatccccaac
     3241 tcaccaaacg tgactgttct gcggaggccg ttccagcgga ctgagcggcg gatgtagggc
     3301 gaaaagccgg cgcgatcgca gcccttgagg gacgggacgt tgtcctcggt cacgaaggtg
     3361 acgacccagc gggcgccccg cgccgccgcg tgctcggcaa tctgggccat cgcgcagggc
     3421 atgatgccct tgccccggtg cttctccggc gtgaatgcgc cctccagcag catctcgtcg
     3481 ggtgtcagac gcgggaagat gttgttgaag aactccctca tccgctcgtt ctcgtccgga
     3541 gagaagagcc actggatgta gcatggctcg ccgtcagcag tggccgccac gaacgggctg
     3601 ccgacgcgct ccagcgccag ctccttgcgg ctggttcgga gctggcgctc ttcgtccgag
     3661 attccgggct ctgtgaggtc gaagaggaat ggccagtcct catcctgaag cggccgcacg
     3721 atcagcggga tctttgcctc cggcgcggtg aaggggacct gcaggtctcg cctgaggcca
     3781 agcgagatcc actctgaccg ccaatgctcg tgtcccttcg cgatctcggt cgcgcggcgc
     3841 atgcccatcc gccgagccgt cgctcgcacg cccttgtacc aaccgttgac gttcatttgc
     3901 ctggaccatt ctgagtacat cgaaagcggc atctggccct tttgtcccac cagcggtggc
     3961 tgaggtcaaa acgggttggt agccgttttg ggggaattgg gaccggcgag tagttggtgg
     4021 atggtagtgg gttgatggat tgcacctcca accaccctca accaaccacc atccacccat
     4081 tcacatctcg cgaaaatact tgcgatcgat ctggcggacg acgtcgtggc tgaagccgcg
     4141 gcgctcgagg tagccggcga ggcggcggcg ctgggtgtcg cggtcgagtc cggtcatctt
     4201 gggcaggcgg cgctcggcga tccgggtggc ggcgtcgagc tcgtcaagat cggcatcggc
     4261 aagtgcttcg tcggcgaggt cggtatcgat ccccttctgg cggagctcct gcttgaggag
     4321 acgggtgccg cgcggcgcga gctgctcccg ggtctggatc cagcggcgcg cgaaatcggc
     4381 atcgtcgaga tagccgagct cgctgaaccg ctcgatgacc tgatcgatga tctgcgggcc
     4441 gaagccccgc tggccgaggg tctggcgtag ctcggagatg ctgcgggggc gataggagag
     4501 ctggcgcgtc gccacctgct tggcagccag gaggtcgtca gaatcgagga gtgcctggat
     4561 ctcagcctgc tccaacgtca ggttaggagc gattcccgca tcgtaggcaa cgttggagtc
     4621 gacggtgtag gtcgtgccgc tctccatcgt gatctcgaca cggcgtccgc ctcggccggc
     4681 cgatgtcacc gacgcgatct tacccgcctc ggcagcgcgc ggcgggcgga caagcggcga
     4741 cttctgacgg cgcatgggtt taatacggaa tgtgctcgac cgggacgccc cgcgcgcaga
     4801 gctcgcaatc gtccggatgg cgcggctcat acagcgtgtt gagcagcccg aacacgggat
     4861 gtccgccgac ctcgtcgcct tgtgagttcc agatagtagc gatgccgacg atgcggccgc
     4921 cgagcccctc gatcacgtca gtgaacttgc tcattgtgac gccgctcatg atcaggttgt
     4981 cgcaaagcag gagccgcttg tcgcggatga gtccctcgag gatcggcgcg acggtcggct
     5041 cgccatcgat cggggtggcg tggacgatcc gggcccgcgg ctcgagaaag taggcaatcc
     5101 actgtgcgac gccggcgccc cagatcgatg gcgtggcaac ggtttcgatc ttgtcggtga
     5161 agaagcgctt ggcgatcgca tatccgaggt ggctggcgaa ctcgggctcg gtcaggagtt
     5221 ggtcgcggtc gatcagccag tgactgtgct ggccggagcg atacgcgaag tggccctctt
     5281 tcaggagtcc gttcgccttg attgccttga cgatcgtcgg gttgagtgcc gccattgccg
     5341 acgcgagtcc tccggttggt gctgtcccca gtccggcgat catacccgag aaggatttgc
     5401 ggtggcgagc ggtggcttac tggaagcgat cccgatcggc ctgcgcggca gtcgccaatc
     5461 gctcaagcga gccgaaatcg agccggaatg cttcgcggca gatgtcggtg cccggcggtc
     5521 cggcctcgac ttcgaccagc aggctgtcgc ccgtgccgga cagtccgacc aagcggtatc
     5581 cacgcgcgtc cagccagcca ccgacagcgc ggagcatatc ctggtagcga ccggagaatt
     5641 ccaggattgg tggggcagac atttcttcga gcgacggcaa ggtccacaca aactgcatca
     5701 ggtcgttcca gtctgagacg tgcatcgacg tgggccgatc cgtctccgct gtcccccggc
     5761 aggagtggtg agccgttacg gtcggtatag cttgatcgta cgtacgcgcg gaaaagcggc
     5821 catggccaaa gcgtccgcaa ggtggcagac caacgtccgg atcatttgcg tccgaaatcg
     5881 ctgatctagg ccgttcgcga ttcgaggcgc cgcgtcagat ccatcaaggg cgccagtgcc
     5941 tgctcgtcca ggccggttgc ggtgagtgcg gccacggcct gatcgtggta tcccgtaacc
     6001 gtctcccgga cccgggcttc gatctggtag cgatccatca ggtcaagtat ttcggcgact
     6061 gcggcgggat cgatctccgg tcggtcatac atttcggcga ggcgagcgcg atcctctggc
     6121 tgcgcgagtt ggcggaggag gaggaccggc agagattgct tcttgcggcg aatgtcgtcg
     6181 gccgcggcct tgcccgtatc ctccgctgcc ccgaaggtac cgagcaggtc gtcctggacc
     6241 tgaaagccga gcccgagcgc aagaccgaac tcggcgaggt gcgccgagag ctcgggaccg
     6301 gcgccaccaa tccaggcgcc accaccggcc gcgaactcga cgatccgggc cgtcttgccg
     6361 gtgatcatct cgatgtactc ggctgaggtt acgtcctggc gctgttcgaa ctggagatcg
     6421 aggacctgtc cggagacgat ctcgatcgtg gtggcgtcga agccacggat aaggtcgatc
     6481 acgacgcttg gcgccacccc gctctcggtc gcgctcagca aagccaggtg agcggcggca
     6541 aagagcgcat cgccggcgtt gattgcctgc gcttcacccc acctggccca gacagtcggt
     6601 cgatgccgtc gtgctggact gttgtcctgg atgtcgtcgt ggatgagcgt gaaattgtgc
     6661 aggagctcga tcgcggccgc gagcggaacg gctgcgtccc cggcggtatc tccaccgacc
     6721 gcccgagccg cgaagatcgc gatcatgggc cgaatcatct tgccccggta ccggtcctgg
     6781 ccggcgaggt cgccagtcca tccgaggtga tactcagcca tctcggcgag gagcggcgcg
     6841 cgctcggcca ataccgagac tgtctggcgc atgttggcca ggcaccgctc agcccaatgg
     6901 ttctcgttca gtggggccgc cattcccgaa ctacttccca ttcgtcacgc gtcgagcgtg
     6961 aaggccgggc gaatctcacc gacgatccgg cgattgtggt tgtagatggt cggatcgtgc
     7021 aatttgagga cgtcgtcgat taccgattcc ggcacgacgt tgagctggcg cagcacttcg
     7081 gccgccacga ccgggtgggc gcggaaggag ccatccgcga ttcggatcgc aattcccagc
     7141 ccatgcgcgg ggatcccgat gcagatcagc ccttcggcgc ccgacttggc cacgatcgtg
     7201 ccgttgccgg ccgccatcag gtcggtcacg aactggccgg gtccacctac attctcgggg
     7261 acgtccatca tggcgtcgcg gaggcgggtc agggcgtcgg catggatgct gggccgggcc
     7321 aggaccgcgt acgagcaggc gaaggcgcgc agggtcgacc caaaggtcgg caggctgcag
     7381 ccatcggtgg cgagcgcgat cgtatcccgc tccacgccgc agacttcagc gattagcccg
     7441 aggatagttt gctggagcgg gtggcaggga tcgaggtagt tgtcgatctg gtagcccagc
     7501 tggaggcacg cggccagcat tccgctgtgc ttgcctgagc agtcgcactg ggtgggcgag
     7561 cgcggtacgt tgcctgcctc aacctgggag aacgcggtct catcgtatgg ggacgggctg
     7621 ccgcattgaa gcgccgtctc atcgagtccg agcttggcga gcatcgccca gacctggcgc
     7681 tggtgctcgg gcgtaccgct gtgcgacgcg caacagaggg cgagctcggc gggcgtgaac
     7741 ccgaaacggt cggccgcgcc ggacgcgata accggaatcg cctggaacgg cttggcggac
     7801 gagcggaagt agaggaagag gtccggatcc ccgaccttgg cgaccagcgc gcccgaactg
     7861 tccacgacgg caatcgagcc gtgatggatg ctttcgatat gatcgccgcg tgtcacgttt
     7921 gccagaactg actgcacctg gatgctcttt cacgagaatt gatcgttcag ccattgtcga
     7981 cttggcactc tacgccaatc cacgcaggca tgccgagtcc ggaaaccagc aactggccga
     8041 tctcgtttaa gagaacatgt taggaagcat ggctgaccga tcaaccagga caccacgaat
     8101 gacgcgattt tcccgacgaa cgctgctggc cggggccggc gtcctgccga ttctgggaac
     8161 cggccgggcc gcgaaagccc agactgaaag cccgatcacg ccgatcgaac catccgagac
     8221 acccaccgac aacggcactg ccaccgtctc gggccgctac ttcggcgata ccggtcacaa
     8281 cctcaaggcg ccgtttctgc gggcgtggca ggcagcgggc ggtcgcgacg ggatcggcgc
     8341 gccgatctcc gaggagcggt tccaggagca cgtcggcgtc acgcagtcgt tccaggcggt
     8401 ctcgctcgtg ttcgatcccg ggctggaatc gccgtgggat atccaggcca cgcatattcc
     8461 ggacgacgtg cgccgcgcga tcgcgcccgg caacgcgcga aaccaggtca gcggcgcgag
     8521 caacaacggg cgctacttcc aggagaccgg gcattcgctg agcggcaaat tcctgaaatt
     8581 ctggaacgag cacggcgatc tccccctgat cgggatgccg acctccgagc cattccggga
     8641 cgcccagacc agcgcgcaac gccaggtctt cgagcgggca gtgctcgagc tcggacagga
     8701 tggcgtcgtc cgcttcctgc cggtcgccgc gcaaatcgcg gaggattccg ggttggccag
     8761 cgatcccgca tttctaccca acccaccggt tgccggcaag acgcaactgg tcaagtcccc
     8821 ggagggactc cgcctgcgag cacgcccaaa cctgtcggcg gacgtgatcg tcgtcctgcc
     8881 cgataacgcc gagtttatcg ccgccgcgga cgagaccggc gactggtacg cggggtacgc
     8941 ggacggattt gccgggtacg tcgcgtccga gttcctgacc gaggcgccac cactgcccga
     9001 aatctcgctg gctgactggg acacgtcagt ttggcagggc gcggccctgg gcgagacgaa
     9061 tctccgtgcc gatccgacca ccaggagcga catcgaccgc gtcctcgagt acggggacga
     9121 ggtgacggtg accgactggg tcaagggtga ggaggtcttt gtcggagcgg atctctgggc
     9181 caggctcaag gatggcgggt acatctacgc ccggaacgtg ggccgcaacg caccggtcgc
     9241 gccgactccc gtcccggccg acgcaccgac cgtcggccgc tggatcgacg tcaacctgac
     9301 gcaacaactg atggttgcgt acgagggccg caacgtcgcc cgcgtgatcg tcacgacgac
     9361 cgggatggcc ggctgggaga cgccgccggg cttctaccag attctctggc gcgtgccgaa
     9421 cgagacgatg gagagcgggg ccatcggcgc cgagcacttc ttcaagctcg aggacgtgct
     9481 cttcacgcag tacttcacca accagggtca cgccatccac tacgcgtggt ggcgcacgcc
     9541 gcagacgatc ggtcggcccg gtagccacgg gtgtctcaat acgttgctcg acgacgcgca
     9601 gtacctctgg gattgggcaa ccatcgggac gcctgtctac gtccataagt aggcgcctca
     9661 cgccgaattc ggctcatgcc ggatagccct tgttggctgt tgtcaacaat tccacgggtc
     9721 gcgtaggctg cggcgatatt gtcctggatt tcgtggtgtg ggggagttgt gatcagggtt
     9781 gggatgcggg ttgcggtact tggattgctg gttgcggcgt tgctcggact ggtcaccggt
     9841 tccgtagcgg ctcaaacggc gaaggcggtg gacgagacgt cgcccacgcc cgggcagaac
     9901 atcacgtata cgattacgat cgacgagtca gccgatcaac acaacggccg ggcaatccgc
     9961 agtgtgacca taacggacca gttgtcggct ggattcacgt tcgtgtcggt cacctgctcg
    10021 ctgtcctgca ccgcgacgac gccggccgtc gggtccgagg gaactgtcac tatcaccgga
    10081 ttcgggcaaa cggaaggccc gttcgatcca acgatgaccg gcacagtgac gctcgtcgtg
    10141 gccgcgtcgg acactgtcgg cgcaacgttc acgaatcagg cctgcatcga tgtcttcgtg
    10201 accccgacga tctccgatcc gcagttctgc gtttcggcgc cggccgttac cgttattgat
    10261 gccccgacgg cgactccgac cgaaacgagc accgccacgc cgacggcaac caatacgcca
    10321 actgcgacca ataccccaac agtcaccgcc accagcaccg ctagtgccac gccgaccaat
    10381 accgttcagc cgacagcgac cagcacggca tcaccgacct cgacagcg
//
LOCUS       DATAOZ010000197         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6131790, whole genome shotgun sequence.
ACCESSION   DATAOZ010000197 DATAOZ010000000
VERSION     DATAOZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6131790"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /gene="recR"
                     /locus_tag="VHR64_05305"
     CDS             <1..441
                     /gene="recR"
                     /locus_tag="VHR64_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX3302278.1"
                     /translation="SKIRLCSRCFNITESDPCEICSDPERDNVICVVEEPLDVVALER
                     TGQLHSRYHVLHGAISPIDGIGPDQLRIKELVNRIEAERPDEVILATNLDLPGDATAT
                     YLHRMLGPSGVRITRPASGLPVGGDLEYADEQTLGRALLARRVL"
     gene            479..1624
                     /locus_tag="VHR64_05310"
     CDS             479..1624
                     /locus_tag="VHR64_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302279.1"
                     /translation="MIERPAKVRVPYLHHSVDPHPVAMDQAAPQHHDSDSRPVTPGPT
                     NVVCRKLRVRDLRHLISNYRLVLVEQPDSLITRTGPLGVALHGLRPFSRMRPVTNLAF
                     TPSDRFLAFAQFRQGAADRRWILTGVGLANGLFAPELVAEQLLEHSVKRAGYRGVKRL
                     FAKVPGDCPIREAFDRCGFERYMAEDIFALDGPLGASPDCDQMREQEPGDTWAVHQLY
                     HAAVPKEVQFAEAWTSHQWDTAGRKHTSASERSFVLEDGHQIAAHARVRTGAKAAAID
                     VMYQPEYRQALEPFCGSVVDRAIREEGATRVFIAVRAYQSELITVLAGMGFARVGQQD
                     LLVKYTAAKVTAKASETIILSPADVRERVPKRVPTFMHRRPKGESSA"
     gene            1749..>3110
                     /locus_tag="VHR64_05315"
     CDS             1749..>3110
                     /locus_tag="VHR64_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3302280.1"
                     /translation="MIDNLDEVLLALPIPIADAVRNELATDRHGPLIEIVLDLGRRPE
                     ARFQNSESYLSDRDVTRDDIEHVVDRIGVFGDDNRAGIERTLHRISAIRNRTGAIVGL
                     TCRVGRAVYGTISIIRDLVESGESVLLLGRPGVGKTTMLREVARVLADDLGKRVVIVD
                     TSNEIAGDGDIPHPAIGRARRMQVPTPTAQHAVMIEAVENHMPEVIVIDEIGTELEAM
                     AARTIAERGVQLIGTAHGNTLENLMMNPVLSDLIGGIQSVTLSDEEARRRGTQKSILE
                     RKAPPTFNVMVEIISRDEVSVHRDVAQTVDTILRGIAARPELRKRSDSGEVEITKAAQ
                     PALPGVSNEPRRMADRDMPRQISREIQPPQGRPSPIQEVIPQPVVAPVSQLPRGRGTQ
                     AKPIRIFPFGVSRNRLEQAITQTRIHATIVRDLREADIVLTLKNYFRQKPQPLRDAET
                     RGIS"
BASE COUNT          604 a          946 c         1020 g          540 t
ORIGIN      
        1 agcaagatca ggctttgctc gcgttgcttc aatatcaccg agagcgaccc gtgcgagatt
       61 tgcagtgacc ccgagcggga caacgtgatc tgcgttgtcg aagagcccct cgatgtcgta
      121 gcgctggagc ggacaggcca gctgcacagt cgataccatg tgctccatgg agcgatctcg
      181 ccgatcgacg ggatcggccc ggatcagctc cggataaagg aactggtcaa ccgcatcgaa
      241 gcggagcggc cggacgaggt gatccttgcg acgaacctcg acctccccgg ggatgcaacc
      301 gcgacgtatc tccaccggat gctcgggccg agcggcgtcc gaatcactcg gccggcgagc
      361 ggactcccgg taggcggaga tctcgaatat gcggacgagc agacgcttgg acgcgcacta
      421 ttggcccggc gcgtgctgtg attgccgggg ggtgacagcc gccctacaat gggcgcacat
      481 gatcgaaagg ccggccaagg ttcgcgttcc gtacctccat cactcagtcg accctcatcc
      541 ggtcgcgatg gatcaggccg ctccccaaca tcacgacagt gactccagac cggtcacgcc
      601 gggcccgacg aatgtcgtat gccggaaact ccgcgtccgt gatcttcgcc acctgatttc
      661 gaactaccgg ctcgtgctgg tggagcaacc cgattcgctg atcacgcgca cgggcccgct
      721 tggcgtcgca ctccatggac ttcgaccgtt cagtcgcatg cggccggtta cgaatctcgc
      781 gttcacgcca tccgatcgat tcctggcgtt tgcccagttc cggcagggag ccgcagaccg
      841 tcgttggatc ctgaccggag tcggtttggc gaacggccta ttcgcgccgg agctggtggc
      901 ggagcaattg ctggagcaca gtgtcaagcg cgcgggatac cgcggcgtca agcgactctt
      961 tgccaaggtg ccgggcgatt gcccaatccg cgaggcattc gaccgctgtg gattcgagcg
     1021 gtacatggcc gaggacatct tcgcgctcga tggaccactc ggtgcatcgc cggattgcga
     1081 ccagatgcgg gagcaagagc cgggcgacac ctgggcggtt caccagctct atcacgcggc
     1141 ggtaccgaag gaggttcagt ttgctgaggc ctggacgagt caccagtggg atacggcggg
     1201 tcggaagcac acctcggcca gcgagcgttc attcgtgctc gaggatggtc accagatcgc
     1261 ggcccatgcc cgcgtgcgaa ccggagcgaa ggctgcggcc atcgatgtga tgtaccagcc
     1321 cgagtaccgt caggcactcg aaccgttttg cggctcagtg gtcgaccggg cgatccgtga
     1381 agagggagcg acccgggtct ttatcgcggt ccgcgcgtat caatcggaac tgatcaccgt
     1441 acttgcgggg atgggtttcg cccgtgtcgg gcagcaggat ttgctggtca aatacaccgc
     1501 cgccaaggtc acggccaagg cgagtgagac aatcatcctc tcaccagcag atgtccggga
     1561 gcgcgtacca aagcgggtgc cgacatttat gcaccggcgg ccgaaaggag agtcgtccgc
     1621 ctgattactt tgattggtcg tattggcgaa tgtctgaatg ccggagaggc gaggacgaca
     1681 cattgataga gacacgagga atcaggcagt tctccgcgaa gtcgcaggga tggccagcgg
     1741 aacaaaagat gatcgataac ctggatgagg tgctgctggc cttgcccatc ccgattgccg
     1801 acgcggtccg gaacgagctg gcgacggatc gccacggccc gctgatcgag attgtgcttg
     1861 acctcgggcg gcgtcccgag gcgcgattcc agaactccga gagctacctg tccgaccgcg
     1921 atgtaacgcg agatgacatc gagcacgtcg ttgaccggat cggggtgttc ggtgacgaca
     1981 accgggccgg tatcgagcgg acattgcacc ggatctcggc gatacgcaat cgcaccggtg
     2041 caattgttgg tctgacatgc cgggttgggc gggcggtcta cggcacgatc tcgatcatac
     2101 gggacctcgt cgaaagcggc gagagcgtac tgctcctcgg tcggccgggt gtcggcaaga
     2161 cgacgatgtt gcgcgaggtt gcccgcgtgc tcgccgatga ccttggcaag cgcgtcgtga
     2221 tcgtcgatac cagcaacgag atcgcgggag acggcgatat tccgcacccg gcgattggtc
     2281 gggcccggcg catgcaggtg cccacgccga ctgcacagca cgcggtcatg atcgaggccg
     2341 tcgagaacca catgcctgag gtcatcgtca tcgatgagat cggcactgag ctcgaggcta
     2401 tggcggcccg taccattgcc gagcgcggcg tccaactgat cggcacagcc cacggcaata
     2461 cgctcgaaaa tctgatgatg aatccggtgc tgtctgatct gatcggcggc atccagtcag
     2521 tcacgctttc cgacgaggag gcgcgacgac gcggaacgca gaaatcgatc ctcgagcgga
     2581 aggcgccgcc gacctttaac gtgatggtcg agatcatcag ccgggatgag gtctcggtcc
     2641 accgcgacgt cgcccagacg gtcgatacga tcctgcgcgg gatcgcggcg cgcccggagc
     2701 tgcgcaagcg gagcgattcg ggcgaggtgg agattacgaa ggcggcccag ccggccctgc
     2761 cgggggtctc gaacgagcca cggcgcatgg ctgatcggga tatgccgaga caaatctccc
     2821 gtgaaatcca gcctccgcag ggtcggccct cgcccattca ggaggtcatc ccgcagcccg
     2881 tagtggcgcc cgtttcgcag ttgccgcgcg ggcgaggcac gcaggccaaa ccgatccgga
     2941 tcttcccgtt cggcgtgagc aggaatcgtc tcgagcaggc aatcacgcag acgcggatcc
     3001 acgcgaccat cgtccgggat ctgcgcgagg cggatatcgt cctgacgctc aagaactatt
     3061 tccggcagaa gccgcagcca ttgcgtgatg ccgagacgcg cggaatttcc
//
LOCUS       DATAOZ010000198         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1398001, whole genome shotgun sequence.
ACCESSION   DATAOZ010000198 DATAOZ010000000
VERSION     DATAOZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398001"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /locus_tag="VHR64_05320"
     CDS             complement(<1..2212)
                     /locus_tag="VHR64_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302281.1"
                     /translation="MKELFGIPMSTFATILVVLLVICLLIVVYIALRKPIVFKMGLRG
                     IPRRPAQTVLIVIGLMLSTLIVTSALGVGDTLDSSVRGFAYDQLGEIDQIIVTSDRGE
                     ADTFTGNYFPETQFADVQSRLAGMPDIDGLLPTIYSSVATINNQTQLANSQVFVTGLD
                     PAYLGPFGGIKSVNGDQIDLNSFPQGTVVVSQALSEDLNLKVGDTFTAYVSGKPTDLA
                     VGAIARNSALLGFGNNPDGTTNSSAFAMALPQAQALLNHQGELNSIFVTMTGGVEQPK
                     ERIEDVNAEAAKRLEGSGLGIYDGKADFLDAAEGISTGFTSIFIVLGLFSIAAGILLI
                     VLIFTMLAAERRPEMGMARAVGQRRLQLIQQFIAEGSGYALAAGIVGAALGIAATFLL
                     GAVLNSLLGDAFPIHATISATSLISAYALGVFITFVTVVAASWKVSRLNIVAAVRDIP
                     DVQASRRRKRTLVWGSLLILLGAGLLLGGTSSDKAFPFYAGISLLPFGIALFARFFGV
                     PSRLAFSLVGLWLVVVWLLPDSVFEKIFGKYDGGIEMFFLSGIFLVIGSTVLIVQNTT
                     TLLRGVTALGGLFKSRLAAVKLAVAYPGQARGRTGMAIAMFSLIIFSLVMIATISQNL
                     TAAFLNEDALAGWDIQADTNIGQPVNDFQGKVAAAPIDQSEITAIGKVTRNNSGLVPM
                     QEIGPTQGPQGSFTLYEMDPAFLDTTKWKFSYRANGYDSDQAIVDAMKADSTLVV"
     gene            complement(2252..3022)
                     /locus_tag="VHR64_05325"
     CDS             complement(2252..3022)
                     /locus_tag="VHR64_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302282.1"
                     /translation="MRTNSNNNHVGPDGSNGFQYMIECQEVVKTYDTGTSKIQALKGI
                     DFAVNQGEMVAVMGPSGCGKTTLLNCLSGLDKVDSGTIHLEGQDLARMSDRKRTDYRA
                     RRMGFVFQVFNLLPVLNAVENVELPLLVSGVKPKIAREKAMNALSKVSLENWASHRPA
                     ELSGGQRQRVTVARSIVNDPAIIWADEPTGALDSSTAQEIIDLMIDLNKRSNLTFVIV
                     THDPGVGAQCDRIVRMIDGQIVGQEIPGHEPLVGVATH"
     gene            complement(3160..>3387)
                     /locus_tag="VHR64_05330"
     CDS             complement(3160..>3387)
                     /locus_tag="VHR64_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302283.1"
                     /translation="AGIPKPVVDRARALLAELESTKSDSPRRTKEPVAQLRFIEEPNP
                     ALDRLAALDVDHLTPVEAITRLYELKSLLDN"
BASE COUNT          640 a         1002 c         1110 g          635 t
ORIGIN      
        1 ccacgacgag tgtggagtcc gccttcatgg cgtcgacaat ggcctgatcg gagtcatagc
       61 cgttcgcccg gtaggagaac ttccacttgg tcgtgtcgag gaaggccgga tccatctcgt
      121 acagcgtgaa gctgccctgt ggcccctgcg ttggaccgat ctcctgcatc ggcacaagtc
      181 cgctgttgtt gcgggtgacc ttgccgatcg ccgtgatctc gctctgatcg atcggagcgg
      241 ccgcaacctt gccctggaag tcgttcacgg gctgcccgat gttcgtgtcc gcctggatat
      301 cccagccggc cagggcatcc tcgttgagga aggcggcggt caggttttgc gagatcgtgg
      361 cgatcatcac gagcgagaag atgatcagac tgaacatggc gatggccatg ccggttctgc
      421 cccgggcctg gccgggatag gcgaccgcga gcttgacggc cgcgagcctt gacttgaaca
      481 acccgccaag cgcggtcacg ccacgcagca gggtggttgt gttctggacg atcagcacgg
      541 tcgaaccgat gaccaggaag atgcccgaga ggaagaacat ctcgatgccg ccgtcatact
      601 tgccgaagat cttctcgaag acgctgtccg gcagcaacca gacgacaacg agccagagcc
      661 cgaccagcga gaatgcgagg cggctcggca cgccgaagaa ccgcgcaaac agcgcgatac
      721 cgaacggcaa caggctgatc cccgcgtaga aggggaaagc tttgtcggag cttgttccgc
      781 ccaggagcag gccggcaccc agcaggatga ggagcgagcc ccagacgagc gttcgcttcc
      841 ggcgtcggga cgcctgcacg tcaggaatgt cgcgcacggc ggcgacaatg ttgagccggc
      901 tgaccttcca cgatgcggcc acgacggtca cgaacgtgat gaagacgccg agcgcgtagg
      961 cggagattag cgatgtcgcc gaaatcgtgg cgtgaatcgg gaaggcgtcg cccagcagtg
     1021 agttcaacac tgcgccgagc aggaacgtgg cggcgatgcc aagcgcggcg ccgacgatac
     1081 ccgcggccag cgcgtatccg ctgccctcgg cgatgaactg ttggatcagt tgcagccggc
     1141 gttggccgac tgcccgggcc atgcccattt cgggacgtcg ctcggccgcc agcatcgtga
     1201 agatgagcac gatgagcagg atgccggccg cgatcgagaa gagacccagg acgatgaaga
     1261 tgctggtgaa gccggtgctg attccttcgg cagcatccag gaaatcggcc ttgccgtcgt
     1321 agatgccgag gcccgaaccc tcaagccgct tggcggcttc cgcattgacg tcctcgatgc
     1381 gttccttggg ctgctcgacg ccgccggtca tcgtgacgaa gatgctgttg agctcgccct
     1441 gatgattcag gagcgcctgc gcctgcggaa gggccatcgc gaaggcggag ctgtttgtcg
     1501 tgccgtccgg gttattgccg aatccgagca gggcgctgtt gcgtgcgatc gcgccgacgg
     1561 ccaagtcggt cggcttgccg ctcacatacg cggtgaacgt atcgcccacc ttcaggttga
     1621 gatcctcgga cagcgcctgg ctgacgacga ccgtgccttg tgggaatgaa ttcaggtcga
     1681 tctgatcgcc attgaccgac ttgatcccgc caaatggtcc aagatacgcg ggatccagac
     1741 cggtcacgaa gacctgcgag ttggcgagct gggtctggtt gttgatcgtc gcgaccgacg
     1801 agtagatggt cggcagcagt ccatcgatgt ccggcatccc ggccagtctg ctctggacgt
     1861 ccgcgaactg cgtctctggg aagtagttgc cagtgaaggt gtccgcctcg cccctatcgc
     1921 tcgtgacgat gatctggtcg atctcgccga gctggtcgta ggcgaacccg cggacggacg
     1981 agtcaagcgt atcgccgaca ccgagcgccg aggtcacaat cagggtgctc agcatcaggc
     2041 cgattacgat cagaaccgtc tgggctgggc gccgcgggat acctcgaagc cccatcttga
     2101 acacaatcgg cttgcgcagt gcgatgtaga cgacgataag caggcagatg acgagcaaca
     2161 cgaccaggat ggtcgcgaag gtcgacatcg ggatgccaaa aagttctttc atgggttgtt
     2221 gtttagtccc cgcgtcgtgc tgacggccga attagtgggt ggccacaccg accagcggct
     2281 cgtgacccgg gatttcctga ccgacgatct ggccgtcgat catgcggaca atgcggtcgc
     2341 actgggcacc gacgcctgga tcgtgggtca caatgacgaa ggtcaggttg ctgcgcttgt
     2401 ttaggtcgat catcaggtcg atgatctcct gtgcggtgga ggagtcgagc gcgccggtcg
     2461 gctcgtcggc ccagatgatg gccgggtcgt tgacgatcga gcgggccacg gtcacgcgct
     2521 ggcgctggcc accggagagc tcggccggcc ggtgcgaggc ccaattctcg agactgactt
     2581 tggagagcgc gttcattgcc ttctcgcggg cgatcttcgg cttgacgccg gagaccagca
     2641 gcgggagctc gacattctcg accgcgttca ggaccgggag caggttgaag acctggaaga
     2701 cgaagcccat ccgccgcgcg cggtagtcag tccgcttgcg atcgctcatt ctggccaaat
     2761 cctgtccctc gaggtgaatc gtgccggagt cgaccttgtc caggcccgag aggcagttga
     2821 gcagcgtcgt cttgccacaa cccgaaggac ccatcacggc caccatctcg ccctggttga
     2881 cggcgaaatc gatgcccttg agcgcctgga tcttcgacgt tccagtgtcg taggtcttga
     2941 cgacttcctg gcactcgatc atgtactgga acccgttcga accgtcgggc ccgacatgat
     3001 tgttgttgct gttcgtgcgc acggtggagc gctctccttg acttcaacgt tcggggaatc
     3061 aatgaaaatg gcacacgggc acgctcgcgg ggcccggcca tctgccagtg tagccgacat
     3121 atcaagtcgt aatgtgaata agttgcgaat gaggaggtcc taattgtcga gcaagctctt
     3181 caactcatag agacgggtta tcgcttcgac cggggtgagg tggtcgacgt cgagcgcggc
     3241 gagtcgatca agcgccggat tcggctcttc gatgaacctg agctgcgcga ccggctcttt
     3301 ggttcggcgc ggcgaatcgg atttggtcga ttccagctcg gccaggagcg ccctggcgcg
     3361 atcgacgacc ggtttgggga tgcccgc
//
LOCUS       DATAOZ010000199         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8764709, whole genome shotgun sequence.
ACCESSION   DATAOZ010000199 DATAOZ010000000
VERSION     DATAOZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8764709"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2083
                     /locus_tag="VHR64_05335"
     CDS             <1..>2083
                     /locus_tag="VHR64_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3302284.1"
                     /translation="LPFADAVAIARQIAEALEVAHERGIIHRDLKPANIKVTPDGDVK
                     ILDFGLAKVVGGDGSMPDLTVVAPTAAGQAGGAIMGTAAYMSPEQARGLAVDKRTDIW
                     AFGCVLYEMLTGRVAFGGDTVSDSIAKILEREPDWSALPATISAPVRRLLVRTLAKDP
                     KNRLRDIGEVRIQIDGLNEAAGILDGPGPRSTARSAWLPWSAAAALAVGLVAMMPGTF
                     RTTPAPMPARFTHLLPAGQMLNGSRGAHIVAVSPDGALLVYSGTPYGLFRRSMSDLDA
                     KVIPGTEGFEVSEPTFSPDGRSIAFFTIGDRALERINTAGGASQVLCPVEAVPTGIRW
                     ESDGIVFGQGSKGVMRVSQNGGTPELLVRVKTGETAYNPQTLPDGDHLLFTLAVGSAP
                     SRWDRARIVVESLRTHHRTLIIDGGSDARYLPSGYLLYALAGAVYAAGFDAARLEVAG
                     ERVPVIEGIRRSAGGFTGAAAFSVSNNGTLVYVPGPAAGERSAPLDVALMDRKGEIQP
                     LHLPGGPYARPKLSPDGTRIAFEADDGNEAVVYTYDLSGAKTMQRLTNGGNNRFPVWV
                     TDQAVAFQSDREGDTAVWWQPLDGSAQRLTRPAAGTSHAPESWFGDKLLYSAANGSDV
                     SLWTYSLQTHHSARFDTVPSSAATDAAFAPDGRSISYSATQGNRMTLYVQGFPTGPAH
                     AFTARTSDTPKH"
BASE COUNT          341 a          719 c          678 g          345 t
ORIGIN      
        1 actgcccttc gcagacgccg tggccatcgc ccgccagatc gccgaagcgt tggaggtcgc
       61 ccacgagcgc ggcatcattc atcgcgacct caaaccggcc aacatcaagg tgacgccaga
      121 cggcgacgtg aaaatcctgg acttcggcct cgccaaagtc gtgggcggtg acggttcgat
      181 gccagatctc acggtggtgg cgccgacggc agccgggcag gccggggggg cgatcatggg
      241 cacggcggcc tacatgagcc ccgaacaggc gcgcgggctg gctgtcgaca agcgcaccga
      301 catctgggcg tttggctgcg tcctctacga aatgctgacc ggacgcgtgg catttggcgg
      361 agacacggtc tccgattcga tcgccaaaat cctcgagcgc gagcccgact ggtcggcgct
      421 gcccgccacg atctccgcgc cggtccgccg cctgcttgtc cgcaccctcg ccaaggatcc
      481 caagaaccgc ttgagggaca tcggcgaggt ccggatccag attgatggcc tgaacgaggc
      541 ggcgggaatc ctcgatggac ctggtccgcg ctcgaccgca cgtagcgcct ggcttccctg
      601 gtccgcggcc gccgcactcg cggtcggtct ggtcgcgatg atgcccggaa cgttcagaac
      661 cacacccgcg ccgatgccgg cgcggttcac gcacctgctg cctgccggcc aaatgctgaa
      721 cggcagccga ggcgcccaca tcgtcgcggt gtcgcccgat ggggcgctgc tagtgtattc
      781 aggaacgccg tacgggctgt ttcgccggtc gatgtcggac ctcgacgcca aggtgattcc
      841 cggcaccgaa ggcttcgaag tcagcgagcc gaccttctcg ccggacggcc gatccatcgc
      901 gtttttcacg attggcgatc gcgcgctcga acggatcaat acggctggcg gagcgtccca
      961 ggtcctgtgt cccgtggagg ccgtgccgac gggcatccgg tgggaatcgg acggcatcgt
     1021 gtttggccag ggatcgaagg gtgtgatgcg cgtgtcccag aatggcggca caccggaact
     1081 gctggtccgg gtgaagactg gtgagaccgc ctacaacccg caaacgctgc ctgacggcga
     1141 ccatctgctc ttcaccttgg cggtgggcag cgcgccgagc cgctgggacc gggcacggat
     1201 cgtcgtggag tcgttgcgga cgcaccaccg aactctgatc atcgacggcg gcagcgatgc
     1261 ccgatacttg ccgtccggct atctcctcta cgcgctcgcc ggagcggtgt atgccgctgg
     1321 cttcgacgcc gcgcgcctcg aagtcgctgg cgaacgcgtc ccggtcatcg agggcatccg
     1381 acgctccgcc ggtggcttca ccggcgcggc ggcattcagt gtctccaaca acgggaccct
     1441 ggtctatgtc ccgggcccag ccgccggtga gcgatcggct ccgctggatg tggccttgat
     1501 ggatcgcaag ggtgagatcc aacccctcca cctgccgggc ggcccatacg cccgtcccaa
     1561 actgtctccc gatggcacgc gcatcgcctt cgaggccgac gatggcaacg aggcggtcgt
     1621 ctacacctac gatctctcgg gagcgaagac gatgcagcga ctcacgaacg gcgggaacaa
     1681 ccgttttccg gtgtgggtca ccgaccaggc ggtcgccttt cagtccgatc gcgagggcga
     1741 caccgcggtg tggtggcagc cgctcgacgg gtccgcgcaa cgcctgacga ggcctgccgc
     1801 gggaacgtcg catgcgcccg aatcgtggtt cggcgataag ctgctctaca gcgcggccaa
     1861 cgggtccgac gtgtcgttgt ggacctattc actccagacg catcacagcg cgcgcttcga
     1921 taccgtgccg tcttccgccg ccaccgatgc cgcttttgcg cctgacggcc ggtcgatctc
     1981 ctattcggcg acccagggaa accggatgac gctctacgtc caggggttcc ccaccgggcc
     2041 ggcccacgcc ttcaccgctc gcacctccga cacccccaag cac
//
LOCUS       DATAOZ010000200         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8502241, whole genome shotgun sequence.
ACCESSION   DATAOZ010000200 DATAOZ010000000
VERSION     DATAOZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8502241"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VHR64_05340"
     CDS             <1..353
                     /locus_tag="VHR64_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302285.1"
                     /translation="PRVVATKNYPFTAVTGLDNNGDTVFNDRPVGVGYNTFRVPGYYA
                     IDLRVARKFPVATDWVLELIIEGFNLANQITPQGATAVNRTWGTGLTPNTTFGQIINS
                     QAARQFQMAFRLTF"
     gene            complement(371..1753)
                     /locus_tag="VHR64_05345"
     CDS             complement(371..1753)
                     /locus_tag="VHR64_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEX3302286.1"
                     /translation="MRRSRPLIALLSLVAATVALAAGPDVHVLMISVDGLMPASYTSP
                     GPARIPVIRELVAEGASAQGVISVLPSVTYPAHTTLVTGVSPAVHGILNNGMFDPEDR
                     SGNASNWYASAIQVPTLLGAAHSSGLKTGAVTWPVTVGMDIDFHVPEFWRTNFGNDHP
                     ESVMFLKVASTPHLLDAIEIARRQPLPGRLTDADRLDMAKFILATYQPNLMLVHFVEL
                     DTMQHNTGLGSPESLATLERIDGYIGTLLKTLTDGGLRERTHVLVVSDHGFLPISKRL
                     QINTLFKQERLLETDDRGRIRKWEAYFYSTGGSGFVFLKNPRDQPLVSRIREMLEKLR
                     ADPANGIRTIWSREEIAQLGSFPDAVLGIDMQSGFYTGGGHDSLLAGVAMNDGRAMSG
                     GHGFDPRRPELYASLVVSGPNARGRGNLGVVRMTQIAPTVARWLGVGLSPLADQPLEK
                     MLPAAGAQPH"
     gene            2008..>2842
                     /locus_tag="VHR64_05350"
     CDS             2008..>2842
                     /locus_tag="VHR64_05350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3302287.1"
                     /translation="MGEVYKARDVRLDRLVAIKVLPADQLTDPESKRRFIQEARAASA
                     LNHPNIVTIYDVGSENGVEFLAMELIEGRTLDELIPRHGFRVPEMLRYAEQAADALAK
                     AHAAGIVHRDLKPGNVMVTGDGLVKVLDFGIAKLVGSSTGQPANVAHSTATASEAGLI
                     VGTASFMSPEQAEGKPVDARSDIFSFGAVLYEMATGQRAFTGDTPMSTISAILRDEPK
                     PLSDFRREIPAELERVIRRCLRKDPARRFQTMADLKVALGELKEESDSGTLAGVPRAT
                     AQ"
BASE COUNT          535 a          920 c          893 g          494 t
ORIGIN      
        1 cgccacgggt cgtcgcgacg aagaactatc ccttcactgc ggtcaccggt ctcgacaaca
       61 acggcgacac cgtcttcaac gatcggcccg tcggggttgg atacaacacg ttccgcgtcc
      121 ctgggtatta cgcgatcgat ctgcgggtgg cgcgaaagtt ccctgtggcc acggattggg
      181 tcctcgagtt gatcatcgag ggcttcaacc tggcgaacca gatcactccg cagggcgcca
      241 cggcggtaaa ccggacatgg ggcacgggtc tcacgccgaa caccaccttc ggccagatca
      301 tcaattcgca ggccgcccgt cagttccaga tggcgttccg actgacgttc tgattgaggg
      361 tgccctctac tcagtgtggc tgtgcgcctg cggccggcag catcttctcc aggggctggt
      421 cagcgagcgg cgacagcccg actccgagcc agcgcgccac cgtcggcgcg atttgcgtca
      481 tacggacgac accgaggttg ccgcggccac gcgcgttcgg ccccgacacg acgagcgacg
      541 cgtacagctc cggacgcctc gggtcgaacc cgtggccgcc gctcatcgcc cggccatcgt
      601 tcatggcgac cccggcgagc aagctgtcgt ggccaccgcc ggtgtagaag ccggattgca
      661 tgtcgatccc gagcacggca tccggaaagc tccccaattg cgcgatctcc tcgcgagacc
      721 agatggttcg tatgccgttg gcgggatcgg cgcgcagctt ttccagcatt tcccggatgc
      781 ggctgaccag tggctggtcg cgtgggttct tcaggaacac gaatccggac ccgcccgtcg
      841 agtagaagta cgcctcccac ttccggatgc ggccgcgatc gtcagtttcg agcaggcgct
      901 cctgcttgaa cagcgtattg atctggagcc gtttgctgat cggcaggaac ccatgatccg
      961 agaccacgag cacgtgggtt ctctcccgca gcccgccgtc ggtcagtgtc ttcaggagtg
     1021 tcccgatgta tccgtcaatg cgctcgaggg tggccagcga ctcaggcgac ccgaggcccg
     1081 tgttgtgctg catcgtatcc agctcgacaa agtgcacgag catcagattc ggctgatacg
     1141 tggcgagtat gaacttcgcc atatcgagcc ggtctgcatc cgtgagacgc cccggcagcg
     1201 gctggcgccg ggcgatttcg atggcgtcga gcaggtgcgg cgtcgatgcc accttcagga
     1261 acatgacgct ctccggatga tcgttcccaa agttggttcg ccagaattcg gggacgtgga
     1321 aatcgatgtc cataccgacg gtcaccggcc acgtcaccgc gccggtcttc aatccgctcg
     1381 aatgagcggc gccgaggagc gtcggaacct gaatggcact cgcataccag ttgctcgcat
     1441 tgcccgagcg atcctccgga tcgaacattc cgttgttcaa gatgccgtgg accgccgggc
     1501 tgacaccagt cacgagtgtg gtgtgcgcgg gataggtcac cgatggaagg acactgatca
     1561 ctccctgggc cgatgcgccc tcggcaacca gttccctgat caccgggatg cgcgcgggtc
     1621 ccggcgaggt ataggacgcc ggcatcaggc catcgaccga gatcatcaac acgtgcacgt
     1681 cgggccccgc ggccagcgcg acggtcgcgg caacgagcga gagcaaagca atgagcgggc
     1741 gtgatcgtct catggcaacc tcacgatggg gacgagacca gacctgagca gccgccggca
     1801 ccggctcaca agcgccgacg acatcataag agcgtgtaac aaaacctcaa aacctgatcc
     1861 tgcgaaagcg ctgagcagcg gcctgagggg ttcccctttt gcagcaaggg ggcagcgtcg
     1921 ctacaatcgc cctcgactct catgtccctg tccgctggca cgcgcctggg accctacgaa
     1981 attctgtccg gcctcggcgc cgggggcatg ggcgaagtct acaaagcgcg tgacgttcgc
     2041 ctcgatcggt tggtcgcgat aaaagtcttg ccagccgacc agctgaccga cccggaaagc
     2101 aaacgtcggt tcatacagga agccagagcc gcctcggccc tgaatcatcc caacatcgtc
     2161 acgatttacg acgtcggatc cgagaacggc gtcgagtttc ttgcgatgga actgatcgag
     2221 ggccggacgc tcgacgagct gataccgcgc cacggttttc gggttcccga gatgctgcgc
     2281 tacgccgagc aggccgcgga cgcgctggcc aaggcgcacg ccgccggcat cgtgcaccgg
     2341 gacctgaagc ctggaaacgt gatggtcacc ggcgacggcc tggtaaaggt gctcgatttc
     2401 ggcatcgcca agctggtcgg gtcgtcaacc ggacaaccgg caaacgttgc gcactccact
     2461 gccacagcct ccgaagccgg gctgatcgtc ggaaccgcat cgttcatgtc gcccgagcag
     2521 gctgaaggca agccggtcga cgcgcgctcc gacatctttt cgttcggggc ggtgctgtac
     2581 gagatggcga ccggccagcg cgcgttcacg ggcgacacgc cgatgtcgac gatctcggcg
     2641 atccttcgcg acgagccgaa gccgctctcc gatttccgcc gcgagattcc tgcggagctg
     2701 gagcgtgtga tcagacgatg cctgcgcaaa gatcccgcgc gccggtttca gacgatggcc
     2761 gatctgaaag tcgcactggg agagctcaaa gaggaatccg attcgggcac actcgccggc
     2821 gtccctcggg cgactgcgca gc
//
LOCUS       DATAOZ010000201         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6397555, whole genome shotgun sequence.
ACCESSION   DATAOZ010000201 DATAOZ010000000
VERSION     DATAOZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6397555"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VHR64_05355"
     CDS             <1..463
                     /locus_tag="VHR64_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302288.1"
                     /translation="LMSKQRTDSSKTYLWCDHCRRSFRHEESRDGACPICGSPMQPTG
                     KMSAILRGLMANELAGSPIVTKHRQIVRLIWTRNGQGEEYYRLLQPKMPYNRFEARVT
                     ELICRGAEEGWISIVIPAAPSDDERNYRVEFLDEDRFLAELDAIAAQPAKS"
     gene            554..907
                     /locus_tag="VHR64_05360"
     CDS             554..907
                     /locus_tag="VHR64_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX3302289.1"
                     /translation="MSTSKGSIVRNERTGQIIGDDIEEAKGLWGSFKGLMFRKTIPDG
                     YGLVFRPARGIHTNFMRFPIDLIYFDKQNRVTKVRPAMKPWRWDFTNAAGVIEMNPGS
                     AARFDVQPGDLLRFE"
     gene            944..1576
                     /locus_tag="VHR64_05365"
     CDS             944..1576
                     /locus_tag="VHR64_05365"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX3302290.1"
                     /translation="METSTSNNRTIFEYLSALRSSSPTPGGGSAAAYAGAMGTSLAAM
                     VCRLTLASADLSTDHPLASAADRLDRLVEELSESASQDEECYSRYRAAAALPRSTPEE
                     RQARGLALQASLHTAAETPLLTARRALEALSLAALVAEHGTKHALSDVDVAISLLTSS
                     IDGALVFVAVNADSIKDENLAADLKRRAEEIRSDASAASKRAATMLGSRA"
     gene            complement(1705..>2996)
                     /locus_tag="VHR64_05370"
     CDS             complement(1705..>2996)
                     /locus_tag="VHR64_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX3302291.1"
                     /translation="PTYDLPIAIAILSASGQVGADLGDALIIGELSLDGIVRHTPGIL
                     AMASLAAQKGLRRVFVPAEDAPEAALIDGIDVYPVRTLADLVNHLTGDVPIGVHRTDA
                     SATEREQVPGTDFSEVKGQEHVKRALEIAAAGAHNVLMSGPPGSGKTMLARAMPTILP
                     EMSVQEALEVTRIYSVRGLLPADVPLMRERPFRAPHHGTSSAGLIGGGSWPRPGEVSL
                     AHRGVLFLDELPEFSASTLEMLRQPLEDRLVTVARASGTVVFPATFTLIAAMNPCPCG
                     FSGDPLRQCTCSSGTVERYQRKISGPLLDRIDIHVQVPRVEIEKLSDKRQGESSTDIR
                     ARVMSARKRQADRFAQTSLLTNADMGPRELNSFVPLDATGEAILKRAVQQLQLSARAY
                     HRVLKLARTIADLDQSDSVLANHLAEALQYRPRIAAA"
BASE COUNT          557 a          976 c          949 g          514 t
ORIGIN      
        1 attgatgagc aagcaacgga ccgattcgag caagacttac ctctggtgcg accactgccg
       61 gcgcagcttc cggcatgagg agagccggga tggcgcctgc ccaatctgcg gctcgccgat
      121 gcagccgacc ggcaagatga gcgccatcct gcggggactg atggccaacg agctggccgg
      181 ctcgccgatc gtgaccaagc accgtcagat cgtgcggttg atctggacgc gcaatggtca
      241 gggcgaggag tattaccgcc tgctccagcc aaagatgccg tacaaccggt tcgaagcgcg
      301 ggtcaccgaa ctgatctgcc gtggggcaga ggagggctgg atctcgatcg tcatcccggc
      361 cgctccgtcc gatgatgagc ggaactaccg cgtcgagttc ctcgacgagg atcgcttcct
      421 ggctgagctg gacgccatcg cagcccaacc agctaaatcc tgacccaggt cgttactccc
      481 ggggtattgc cccacccgtc atcacccgat agggtgacag gaacgcgttg agacttcggg
      541 acggggagac tgggtgagca cgagcaaggg atcgatcgtt cgcaacgagc gcacgggtca
      601 aatcattggc gacgacatcg aagaggcaaa agggctctgg ggcagcttca aggggttgat
      661 gttccgcaag acgattcccg acggctacgg cctcgtgttt cgtccggctc gcggcatcca
      721 caccaacttc atgcgctttc cgatcgacct gatctacttc gacaagcaaa accgggtcac
      781 caaggtgcgg cccgccatga agccatggcg ctgggacttc acgaacgcgg ccggcgtcat
      841 cgagatgaat ccgggatcgg cggcccgatt cgacgtccag ccaggcgatc tgcttcgctt
      901 cgagtaacag gcgcatttcc ggcggcttcg atactatggc ctcatggaaa cgagcacttc
      961 caacaaccgg actatcttcg agtacctgag cgccttgcgt tccagttccc ccaccccggg
     1021 cggtggcagc gcagccgcct atgccggcgc gatgggcaca tcgctggcgg cgatggtctg
     1081 ccgactgacc ctcgcgtcgg cagacctttc aactgatcac ccgctagctt cggcggccga
     1141 tcggttggac aggctcgtcg aggagctctc cgaatcggcc agccaagacg aggagtgcta
     1201 ttcccggtac cgcgctgccg ctgctcttcc aaggtcgacg ccggaggaga ggcaggcacg
     1261 cggcctggcg ttacaagcct ccctgcacac cgccgccgaa acgccactcc tgaccgcgcg
     1321 acgcgctctc gaggcactca gcctcgccgc gctggtcgcc gagcatggca cgaagcacgc
     1381 cctgtccgat gtcgacgtcg cgatatcgct cttgacatcg tcgatcgacg gcgcgttggt
     1441 cttcgtagcg gtcaatgccg actcgatcaa ggacgaaaac ctggcggctg acctcaagcg
     1501 ccgagccgag gaaatccggt ccgacgcttc ggcggcctcg aaacgcgcag ccaccatgtt
     1561 gggatcccgc gcctgagaag tcccccatcg ctttgcgccg aaccaaccta gtaccggacg
     1621 gctcgcccgt tctaccgctg acgggaatcc gggcgagccg cccagcgcta catcagatgt
     1681 acggcatgtc ctcagaatcg tgctctatgc ggcggcgatt cgcggacgat attggagtgc
     1741 ttcggccagg tgattcgcca ggaccgagtc ggattgatcg aggtcggcga ttgtccgggc
     1801 cagcttcagc acgcgatgat aggcgcgggc ggagagctgc aactgctgca ctgcccgctt
     1861 caggatcgct tcgccagtcg cgtcgagcgg cacgaacgag ttcaactcgc gtggacccat
     1921 gtcggcgttg gtcaggagcg atgtctgcgc gaagcggtcc gcctgccgct ttcgggcaga
     1981 catgacgcgg gcgcggatgt cggtcgatga ctcgccctgt cgcttgtcgc tcagcttctc
     2041 gatttcgaca cgcggcacct ggacatggat gtcgatccga tccagcagcg gtcccgaaat
     2101 cttgcgttga taccgctcga ccgtccctga cgagcaggtg cactgtcgca gtgggtcacc
     2161 tgaaaacccg catgggcaag gattcatggc cgcgatcagc gtgaaagtgg ccggaaaaac
     2221 gaccgtgccg gatgcccggg ccacggtcac cagccgatcc tcgagcggct gacgcagcat
     2281 ctcgagcgtg ctggccgaga actcgggcaa ctcgtcgagg aagagcacgc cgcggtgcgc
     2341 gagcgaaacc tctccgggtc gcggccagga tccgccgccg atcaatccgg ccgagctggt
     2401 cccgtggtgc ggcgcgcgaa acgggcgctc gcgcatcaac ggaacatcgg ccggcaagag
     2461 accccgcacg ctgtaaatcc gcgtgacctc gagtgcctcc tgcaccgaca tctccggcag
     2521 gatggtcggc atcgcacggg ccagcatcgt cttgccggaa cccggtgggc cggacatcag
     2581 gacgttgtgg gcacccgccg cggcgatctc gagcgctcgc ttgacgtgct cttgcccttt
     2641 gacctcgctg aagtcagttc ccggaacttg ctcacgctcg gttgccgagg cgtcggtccg
     2701 atgcaccccg atcggcacgt cgccggtcag atgattgacg aggtcggcca gggtgcgcac
     2761 cgggtaaacg tcgatcccgt cgatcaaggc agcctcgggc gcatcctctg ccggcacgaa
     2821 gacccgacgc aaccccttct gggcggccag tgatgccatg gcgagaatac cgggcgtgtg
     2881 ccggacgatc ccgtccagtg acaactcccc tatgatcagg gcgtctccga ggtccgcgcc
     2941 gacttgccca gacgctgaca gaatcgcgat ggcgatcggg agatcgtagg tcggtc
//
LOCUS       DATAOZ010000202         9185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8241841, whole genome shotgun sequence.
ACCESSION   DATAOZ010000202 DATAOZ010000000
VERSION     DATAOZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8241841"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /gene="gatB"
                     /locus_tag="VHR64_05375"
     CDS             <1..1052
                     /gene="gatB"
                     /locus_tag="VHR64_05375"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX3302292.1"
                     /translation="SRNETGQEISLVDLNRSGVPLMEIVGDPDLRTAQQAGDYLTSLR
                     AILRSIGASTGNMEEGAFRCDANVSVRATDGSFAGPKVEIKNMNSFRAVVRALRFEEE
                     RQRDVLANGGTLVQETRGWSEATGTTVSQRTKEQAHDYRYFPEPDLPPLTMTPDRLDL
                     IRDTIGELPVARRDRYVAAYDIARADAGLIAYDEALANYFEGVLGPDADSKRARTSAN
                     WVLNDVLGLQRSRSMPGEDFPVSADQVRVLIDLVESGEMTGRAAKELLPQLAFGEPVR
                     DAANRLSLLSLDDQDAVRSAARAAIEAFPAAVEDYRRGKTAAIGRLIGETIKNTGGRA
                     KPDDVRAILTEELSR"
     gene            1114..2403
                     /locus_tag="VHR64_05380"
     CDS             1114..2403
                     /locus_tag="VHR64_05380"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX3302293.1"
                     /translation="MVAVVDRPATRPADHHGRDLFDVAVEQFNRAADVVGLEDSMRRI
                     LSVCQREVTVSFPVEMDDGSVQVFTGHRVQHNTGPGPTKGGIRFHQDVTLQEVKALAM
                     FMTWKCAVVGLPYGGAKGGVTVNPKMLSQTELQNLTRRYTTEIQFMIGPNQDIPAPDV
                     NTNAQIMAWLMDTYSMNVGYSVPGVVTGKPIALGGSQGRADATGRGVVFALEEAAPKI
                     GLNLNGATAVVQGFGNVGSVATRLLQQVGVKVVAIGEVYASIYNPDGIDIDKLIEYRA
                     KNGRINDFPGAELVPTDELLELPCDILVPAALENQIDSDNAPKIKARIIVEGANGPTS
                     PEADDILADRGIMVVPDILANAGGVTVSYFEWVQALQAFPWTEQQVNERLKQIMTNSF
                     NAVYEASEKFNVSLRTGALAYAVTRVAEFTQLRGIYP"
     gene            complement(2425..3078)
                     /locus_tag="VHR64_05385"
     CDS             complement(2425..3078)
                     /locus_tag="VHR64_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX3302294.1"
                     /translation="MYIIVVGGGKVGFYLTKTLLAEGFEVLLIERDPSKVDYFNEQLG
                     GVALRGDGAESSVLDAAGAGRADVVIAVTGEDEDNLVVCQMAKIAFKVEKTIARVNNP
                     RNEPIFKMLGIDVTVSHTAHIMNIIEQAIPETPYIHLLNLQHPDLAIVDLKVGHDSPV
                     NGVAMGDIDLPEDCIIAAVLRGPDLIIPADTTELRAGDDVIAITHADQEDALRRLLI"
     gene            complement(3082..3498)
                     /locus_tag="VHR64_05390"
     CDS             complement(3082..3498)
                     /locus_tag="VHR64_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX3302295.1"
                     /translation="MKVVIVGCSRLGARLADSLDREGHSVTVIDVDSTAFRRLQPSFS
                     GSTVIGTGIDEDILLTAGTDTADVFIAATNGDNRNIMAAQLAKHAFRVNHVLCRIYDP
                     VRAETYRSLGIDTICPTVQVANLFLQAISAETAKVE"
     gene            complement(3495..3968)
                     /locus_tag="VHR64_05395"
     CDS             complement(3495..3968)
                     /locus_tag="VHR64_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3302296.1"
                     /translation="MGKQVSDFQRVLVPVSGAPGDAHALKIVSRLIEPKRAEVTAVYV
                     IEVAQSLPLDAELPDEIDRGERALQSAEEVARQYCGTRSDFISTDLLQARSAGAAIVD
                     EASERQCDLIVLTSMCQQIHGKRHVSETVSYVLKNAPCEVMLLSVPMSTNGQSAS"
     gene            complement(4046..4777)
                     /locus_tag="VHR64_05400"
     CDS             complement(4046..4777)
                     /locus_tag="VHR64_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX3302297.1"
                     /translation="MKVLLISDVHANLVALDAVLKDAGEWDAVWNLGDTVGYGPRPRE
                     CLDKMVDLFANPVLVGNHDLACIGEVDISEFNPVAQMAARWTSLQLGMDHRAYLNGLP
                     AVTMANSYTLAHGSPRSPIWEYVTSAQTATENFGFFDTDVCFIGHTHIAMYALLKEGE
                     ETAQIAALQHEEKLDLLAGRFLINPGSVGQPRDRDPRAAYAILDTDLGSLTAKRVEYD
                     ISATQRQMAMANLPEVLITRLAHGM"
     gene            complement(4774..6717)
                     /gene="mnmG"
                     /locus_tag="VHR64_05405"
     CDS             complement(4774..6717)
                     /gene="mnmG"
                     /locus_tag="VHR64_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX3302298.1"
                     /translation="MTLECDLVVIGAGHAGCEAALAAARTGARVLVVTPNLDRVGFMP
                     CNPSIGGPGKSHIVAEIDALGGAMAEVADQTAVQVRRLNTSKGPAVQAIRHQIDKGLY
                     SVVMKQRLEEQSGLSLVQDDAIGLELEAGAVRSVRLRSRGVVPCRAVVVTAGTFLRAA
                     MISGESRQTGARAGDSADQQLAANLQELGVATRRFKTGTPPRIDGRTVDLGRLEPQPG
                     DAEPSWLSRKGRLGTLDLVSLPPVALHRDHATLNGWRPQLSCYRTSTHPVIHELIRAN
                     LDRAPMFNGLIEGVGPRYCPSIEDKVARFVDKDEHPIFLEPEGWRTNELYVQGMSTSL
                     PFDVQQRALRMIPGLEQVAITRFGYAVEYDAIDPNSLSPTLESRQIAGLFLAGQVNGT
                     SGYEEAGGQGLIAGLNAARYVVGEPLVTIPRSLGYIGVMIDDLVSVPFTEPYRMLTAR
                     AEYRLSLRTDTAADRFRERACRWQLIDDARCIALADERAQLNRAVRAFDETSLNPNSA
                     EDRALQEIGQSPIGKPMSLAALMRRPSLSLAELREALPALVDPLTAAMQPHVLRRFED
                     ELKYAAFVAREQREVTRTETLAERSLPDTDCSIPGLRHEARQQIERHRPRTFGEAQRI
                     AGITAADISALLIHATRLEHAQQ"
     gene            6927..7613
                     /locus_tag="VHR64_05410"
     CDS             6927..7613
                     /locus_tag="VHR64_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302299.1"
                     /translation="MSNLNSESIGAVRDQAAQLLQSVIDQATARAMEQARATKKQSGR
                     LSSRLPGSKSAPTVRDVALNAATAAIELWQSARDRAGDTVGSVQSTVTDSAADIKHSA
                     QGVTSGAAQRVAGVKDSAQGVTSDAVHRVAGVKDDAVDRVKSVSHAVGDLPHKAADTS
                     KAAATTTAKTGRNMFGLVVWTGAAGAIIYYAFLNEERREQVRQIAMQAISEARTLLQD
                     LQGQDGEFAS"
     gene            complement(7589..>9185)
                     /locus_tag="VHR64_05415"
     CDS             complement(7589..>9185)
                     /locus_tag="VHR64_05415"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX3302300.1"
                     /translation="IVDEFTGRMMVGRRYSEGLHQAIEAKEGVRVRRQNVTMATITFQ
                     NYFRMYKKLAGMTGTAKTEDEEFHRIYSLEVVVVPTNKEMIRDDAADYVFKNESGKFR
                     AVIRDIEEKQEHGRPVLVGTTSVETSERLSNMLTARGIEHEVLNAKHHEREANIVALA
                     GQPRAVTIATNMAGRGTDIVLGPGVTEAGGLHIIGTERHESRRIDNQLRGRSGRQGDP
                     GSSRFYVSLDDELMRRFGGERIVGLMERLGMEEDVPIEHRVISKSIESAQTKVEGHNF
                     DIRRHVVQYDDVMNRHREVIYADRRAIVKGEDMSEKIEEMVLGEIEDIVERNSDEKSR
                     EINDTAIVEGYKALIPNGNVDQEQLEGLDEEGIIHVLTEDALRAYQEVEDRFGPEIMR
                     KVERQIMLAVMDRLWVEHLTEMDDLREGVGLQAYGQRDPLVVYKTEGYRMFQQLLDHI
                     QHDVVHTLYRVQPAVAEQPVKTRMSQEVTTNRESENGNAPARSRKIGPNDKCFCGSGK
                     KYKFCHGAPAGKRKIAQEANSPS"
BASE COUNT         1697 a         2831 c         2878 g         1779 t
ORIGIN      
        1 attccagaaa tgagactggc caggagatta gcctggtcga tctgaaccgg tccggcgtcc
       61 cgttgatgga gatcgtcggc gaccccgatc tgcgcacggc ccagcaagcg ggtgactacc
      121 tgacgtcact gcgggcgatc ttgcgctcga tcggcgcctc gaccggcaat atggaagaag
      181 gcgccttccg ctgcgacgca aatgtctccg tgcgcgcgac cgacgggtcc tttgccggcc
      241 ccaaggtcga gatcaagaac atgaacagtt tccgcgcagt cgtgcgggcg ctgagattcg
      301 aggaggagcg ccagcgcgac gtactggcaa atggcggaac gcttgttcag gagacgcgtg
      361 gctggtccga agcgaccggc acgaccgttt cgcagcggac aaaggagcaa gcgcacgact
      421 accggtattt ccccgagccg gatctgccgc cgctgacgat gactcctgac cgcctcgatc
      481 tgatccgcga cacgatcggc gagttgccgg ttgcgcgtcg cgatcgatat gtcgctgctt
      541 acgacatcgc gcgcgcagat gccggactca tcgcatacga tgaagccttg gccaattact
      601 tcgagggggt gctcggtccc gatgcggaca gcaagcgggc acgcacctcc gccaactggg
      661 ttttgaacga tgtcctcggc ctgcagcgct cgcgatcgat gccgggggag gacttcccgg
      721 tctcggccga ccaggtgcgc gtgttgattg acctggttga gtccggtgag atgaccggcc
      781 gggccgcaaa ggaattgctg ccccagctcg cattcggtga gcccgtgcgg gacgctgcca
      841 accggctgag cttgctttcg ctcgatgatc aggatgcggt tcggtccgcc gcgagagccg
      901 cgatcgaggc atttccggcc gcggttgagg actatcggag gggcaagacc gctgccattg
      961 gtcgcctgat cggtgagacg ataaagaaca ccggcggtcg cgccaagccc gatgacgttc
     1021 gggccatcct aaccgaggag ctgtcacgtt agggttttcg gacgtggccc gcgaagaagc
     1081 gggcaaaacc tgccgaagga ggcaaatatc accatggttg cagtcgtcga tcgtccggcc
     1141 acaagaccag ctgaccacca cggacgcgac ctgtttgacg ttgccgtcga gcaattcaat
     1201 cgcgccgcag acgtcgtggg gctcgaagac tcgatgcgcc gcattctcag cgtgtgtcag
     1261 cgagaggtga cggtcagctt tccggttgag atggacgacg gttcggtcca ggtcttcact
     1321 ggccaccgcg ttcagcataa caccggacca ggcccgacca agggtggtat tcgctttcac
     1381 caggacgtga cgctccagga ggtcaaggcc ctggcaatgt tcatgacctg gaagtgcgcg
     1441 gtggtcgggc tgccgtacgg tggcgccaaa ggcggcgtga cggtcaaccc caagatgctc
     1501 tcccagaccg agctgcagaa cctgacccgc cgctacacga ctgaaatcca gttcatgatc
     1561 ggaccgaacc aggacattcc ggcgccggac gtcaacacca atgcccagat catggcctgg
     1621 cttatggaca cctattccat gaatgtcggc tattcggtgc ctggcgtcgt gacgggcaag
     1681 ccgatcgcac tcggaggctc gcagggtcga gcggacgcga ctggcagggg ggtcgtgttt
     1741 gccttggaag aggcggcgcc gaagatcggc ctgaacctga acggagcaac cgcggtcgtg
     1801 cagggtttcg gaaatgtcgg atctgtagcg acccgattgt tgcagcaggt cggcgtcaaa
     1861 gtcgtcgcga tcggcgaggt ctacgcgtcg atctacaatc cagatggcat cgatatcgac
     1921 aagttgattg agtaccgcgc caaaaacggc cggatcaatg actttccggg tgccgagctg
     1981 gtcccgaccg atgagctact cgagctccca tgcgacatcc tggtgccggc cgcgctggag
     2041 aatcagatcg attcggacaa tgcgccgaag atcaaagcca ggatcatcgt cgaaggcgcg
     2101 aacggtccga cttcgccaga ggcagacgac atcctggcag accgcggaat catggtcgtg
     2161 ccggatattc tcgccaacgc gggcggcgta accgtctcgt acttcgagtg ggttcaagcg
     2221 ttgcaggcgt tcccgtggac cgaacagcag gtcaatgagc ggctcaagca gatcatgact
     2281 aactcattca atgcggtcta cgaggcatcg gagaagttca atgtcagtct gcggaccggc
     2341 gcactcgcct acgccgtgac ccgggtcgcc gagttcacac agctccgggg catctacccg
     2401 taatcgactt cgaaggcgga acgatcagat caggaggcgc cgcagggcgt cttcctgatc
     2461 tgcgtgcgtg atcgcgatca cgtcatcgcc ggcacgcagc tcggtcgtgt cagccgggat
     2521 gatcagatcg gggccgcgca gaacggccgc gatgatgcag tcctcgggca gatcgatgtc
     2581 gcccatcgcc acgccgttga cgggcgagtc gtgcccgacc ttgaggtcaa cgatcgcgag
     2641 gtccgggtgc tggaggttga gcagatggat gtacggcgtc tccgggatcg cctgctcgat
     2701 gatgttcatg atgtgcgccg tgtggctgac cgtgacatcg ataccgagca tcttgaagat
     2761 cggctcattt ctcgggttgt taacacgcgc gatcgtcttt tcgaccttga aagcgatctt
     2821 ggccatttgg cagacgacga ggttgtcctc gtcctcgccg gtaaccgcga tcaccacatc
     2881 ggcccgacca gctcccgcgg catccagcac cgacgactcg gcgccgtcgc cacggagggc
     2941 aactccaccg agttgctcgt tgaagtaatc gaccttggat gggtcccgct cgatcaggag
     3001 aacctcgaag ccttcggcga gcaaagtctt cgtcaggtag aagccgactt tgcctccgcc
     3061 gaccacaatg atgtacatcg cctactcaac cttcgccgtt tcggcagata tggcctgcag
     3121 gaagaggttc gcgacctgta ccgttgggca gatcgtgtcg atccccaggg accggtacgt
     3181 ctcggcccgg accggatcgt agattcggca gaggacgtgg ttgaccctga atgcgtgttt
     3241 ggcaagctgg gcggccatga tgttccggtt gtcgccattg gtcgccgcga taaacacatc
     3301 ggccgtgtcg gtgccggcgg tcaggaggat atcctcatcg atgccggtgc cgatgactgt
     3361 cgatccggag aatgacggct ggagccgccg gaacgccgtg gagtcgacat cgatcacggt
     3421 gaccgagtga ccctcgcgat caagcgagtc ggccaatcgc gcaccgagtc ggctgcaccc
     3481 tacgatgacg accttcacga tgcggactgc ccgttggtgc tcattggcac actcaggagc
     3541 atgacctcgc acggagcatt cttgagcacg tagctgaccg tctcgctgac gtggcgcttg
     3601 ccgtggatct gctggcacat cgacgtcagc acgatcagat cgcactgtcg ctccgaggcc
     3661 tcgtccacga ttgccgcgcc ggcagaccgc gcctggagca ggtcggttga gatgaagtcg
     3721 gaacgcgtac cgcagtattg acgggcgact tcttccgccg attgcagtgc gcgctcgccg
     3781 cgatcgattt cgtcaggcaa ttcggcgtcg agcggcaggg actgcgcgac ctcgatgacg
     3841 tagacagcgg tgacttcagc gcgcttcggc tcgatcagac gactgacgat cttgagggcg
     3901 tgcgcatcgc ccggcgcgcc actgaccgga acgagcaccc gctgaaaatc cgatacctgt
     3961 ttacccatga tgttaatttc ttgaactccc cattggctca gcaagccaac atggaattat
     4021 gacgcccgga tgtgactact gcaactcaca ttccatgcgc cagacgcgtg atcagaacct
     4081 ccggcaaatt agccatggcc atctgccgct gcgtcgccga gatgtcatat tcgacccgtt
     4141 tcgcggtcaa tgatcccaga tcggtgtcga gaatggcata ggcggcgcgc ggatcgcggt
     4201 cacgcggctg accgacgctg ccgggattga tcaggaaacg accggcaagc aggtccagtt
     4261 tctcctcgtg ctgcagagcg gcaatctgcg ctgtctcctc gccctccttg agcagcgcat
     4321 acattgcgat gtgcgtgtgg ccgatgaaac agacatcggt gtcgaaaaag ccgaagttct
     4381 ccgtcgcggt ttgggccgaa gtcacgtact cccagatcgg cgaacgcggg ctgccgtgcg
     4441 ccaatgtgta cgagttggcc atcgttaccg cagggagtcc attcaggtag gcgcggtgat
     4501 ccatgccgag ttgaagcgaa gtccatcgcg ccgccatctg cgccaccggg ttgaactcgc
     4561 tgatatcgac ctcgccgatg caagccagat cgtggttgcc gaccagcacc gggttcgcga
     4621 acagatcgac catcttgtcg aggcactcgc gcggccttgg tccgtacccg acggtatcgc
     4681 cgagattcca gactgcatcc cactctccag cgtccttgag gacagcgtcg agcgcgacga
     4741 gattcgcgtg gacgtcggaa atcagcagaa ccttcactgt tgggcgtgtt ccaatcgtgt
     4801 cgcatgaatc agaagcgccg agatgtctgc cgcagtaatc ccggcgattc gctgcgcttc
     4861 tccgaaagtc cgcgggcgat ggcgctcgat ttgttgccgg gcttcgtgcc gaagaccggg
     4921 gatcgagcaa tcggtgtcgg gcaggcttcg ctcggcgagc gtttcagtcc gggtgacctc
     4981 tcgctgctcc cgggcgacaa atgccgcgta tttcagctcg tcttcgaacc ggcgaagcac
     5041 gtgcggctgc atggcggcgg ttaacgggtc gacgagcgcc gggagtgcct cccgcaactc
     5101 tgcgagggag agcgagggac ggcgcatcaa tgccgccagc gacatcggtt tgcctatcgg
     5161 actctggccg atctcctgga gcgcccggtc ctcggcactg ttcgggttca gggatgtttc
     5221 gtcgaaggcc cggacagcgc ggttgagctg ggcgcgctca tccgcgagcg caatacaccg
     5281 cgcgtcgtcg attaattgcc accggcacgc tcgctcccgg aagcggtcgg cggccgtgtc
     5341 tgtccgcagt gaaaggcggt attcggcccg tgcggtcagc attcggtacg gctcggtaaa
     5401 cggaaccgag accagatcgt cgatcatcac accgatgtat ccgagtgacc gtggaattgt
     5461 gacaagtggc tcgcccacca catagcgggc ggcgttgagc ccggcgatca ggccctgccc
     5521 accggcctcc tcgtagcccg acgtgccgtt gacctgccca gcgaggaaca gacccgcgat
     5581 ctgacgcgac tccagcgttg gagacagcga attcgggtcg atggcgtcgt attcgaccgc
     5641 gtatccgaag cgcgtgattg cgacctgctc gaggcccggt atcatcctga gcgcccgctg
     5701 ctggacgtcg aatggcaggc tcgtgctcat gccctggacg tagagctcgt tggttcgcca
     5761 tccctcgggt tcgaggaaaa tcggatgctc gtctttgtcg acgaatcgcg cgaccttgtc
     5821 ctcgatggat gggcagtaac gcgggccgac gccctcgatc agcccgttga acatcggcgc
     5881 gcggtcgaga tttgcccgga tcagctcatg tattacggga tgcgtagacg tccggtagca
     5941 cgaaagctgc ggcctccatc cgttcaaggt cgcgtggtcc cggtgcagag caacgggcgg
     6001 cagcgagacg agatcaagcg tgccaagcct gcccttccgg gacagccagc ttggctccgc
     6061 atcgccgggt tgtggctcga gacggcccag atcgaccgtg cggccatcga ttcgtggcgg
     6121 cgtaccggtc ttgaaccggc gagtcgcgac gccgagctct tgcaggtttg ccgccagctg
     6181 ctgatcggcc gaatccccgg cccgggcgcc ggtctggcgg ctttcgcccg atatcatcgc
     6241 ggcgcgcagg aaggtcccgg cggtaacgac tacggcccgg cagggaacga cgccacggga
     6301 tcgcagacgg actgatcgaa ccgcaccagc ttccagctcg agaccgatcg cgtcgtcctg
     6361 gacaagcgag agtcccgact gttcctccag tcgctgcttc atgacgacgg agtagaggcc
     6421 cttgtcgatt tggtggcgaa tagcctggac agccggtccc ttgctcgtgt tcagccggcg
     6481 aacctggacc gccgtctggt ccgcaacttc ggccatcgcg ccgcccagcg cgtcgatctc
     6541 ggccacgatg tggctcttgc ccgggccgcc gatcgaaggg ttgcagggca taaagccgac
     6601 gcggtcgaga tttggcgtga ctaccaacac gcgcgcgccg gttcgggccg ccgccagggc
     6661 agcctcgcag cccgcgtggc ccgcgccgat gacgaccagg tcacattcca atgtcatatc
     6721 gtcaggtcgg gacatgtacg ttcgtagtac tccgcactat cgctgcgcca ttgtacgttg
     6781 gcagccgcat tccactgcca ctttgcatcc tccgatggtc atgactctgc aagctctttg
     6841 ttatccgcgc tgttgatcgg tgcacagctc atcggctact gtcatttgca gcgaatggct
     6901 attcggatcg taaaggacgc agagcaatgt caaatctgaa ctctgaatcg atcggagcgg
     6961 tccgagatca ggccgcccag ctgctccaat ctgtgattga tcaagcgact gctcgcgcga
     7021 tggaacaggc gcgggccacc aagaagcaga gcggacggct gagcagccgg ttaccaggga
     7081 gcaagtccgc cccgacggtg cgggacgtcg cattgaatgc ggccaccgca gccattgagc
     7141 tctggcagtc cgcccgcgat cgagccggtg acacggtcgg atcggtccag tcgactgtga
     7201 ccgactctgc agccgacatc aagcacagcg cacagggtgt gacctcgggc gcggcacagc
     7261 gggtcgcggg ggtcaaggac agcgcacaag gtgtgacctc agacgccgtg catcgggttg
     7321 ctggcgtcaa ggatgatgcc gttgaccggg tcaagtcggt tagccatgcg gtcggtgatc
     7381 tgccgcacaa ggcagcggat accagcaagg ccgcagccac gacaaccgcg aaaacagggc
     7441 gcaacatgtt cggtctcgtg gtctggaccg gcgccgccgg cgccatcatc tactacgcgt
     7501 ttctaaacga agagcggcgc gagcaggtgc gccagatcgc gatgcaagca atcagcgagg
     7561 cgcgcacctt gctccaggat cttcagggtc aggacggcga gttcgcctcc tgagcaatct
     7621 tccgcttgcc ggcaggagcg ccgtggcaga acttgtattt cttaccgctc ccgcagaagc
     7681 acttgtcgtt cggcccgatt ttccgtgatc gggccggcgc gttcccgttc tccgattcgc
     7741 ggttggtcgt cacttcctgg gacatccggg tcttgaccgg ctgctcggcc acggcgggct
     7801 gcacgcggta gagggtgtgc acgacatcgt gctggatatg atccaggagc tgctggaaca
     7861 tccggtagcc ctcggtctta tagaccacca gagggtcgcg ctggccgtag gcttgtaggc
     7921 ccacgccctc gcgcaggtca tccatctcgg tgagatgctc gacccaaagc cgatccatta
     7981 cggccagcat gatctggcgc tcgaccttgc gcatgatctc gggcccgaag cgatcttcga
     8041 cctcctgata cgcacgcaaa gcgtcttcag tcaggacgtg gatgatgcct tcctcgtcga
     8101 ggccctcgag ctgttcctga tccacatttc cgttggggat cagcgccttg tatccctcga
     8161 caatcgcggt gtcgttgatc tcgcgagact tctcatcgga gtttcgctcg acgatatctt
     8221 cgatctcgcc gagcaccatc tcctcgatct tctcgctcat gtcttcgccc ttgacgatcg
     8281 ctcgccggtc cgcgtagatc acttcgcggt gccggttcat gacatcgtcg tactggacga
     8341 catggcgacg gatatcgaag ttgtggccct cgaccttcgt ctgggcgctc tcgatcgact
     8401 tgctgatcac gcggtgctcg atcggcacgt cctcttccat gccgagccgc tccatcaggc
     8461 cgacgatacg ctcgccgccg aacctccgca tcaactcgtc atcgagcgag acgtagaagc
     8521 gcgatgatcc agggtcgccc tgacggccgg accgtccacg gagctgattg tcgatccggc
     8581 gcgattcgtg gcgctcggtg ccaatgatgt gcaaaccgcc ggcctcggta acgccgggtc
     8641 caagcacgat gtcggtacca cgaccggcca tgttggtcgc aatggtgact gcgcggggct
     8701 gtccggccaa cgcgacgatg ttcgcctcac gttcgtgatg tttcgcgttg aggacctcgt
     8761 gttcgatacc ccgcgcggta agcatgttcg aaagccgctc agaggtctca accgaggttg
     8821 tgccgaccag aaccgggcga ccgtgctcct gcttttcttc aatgtcgcgg atcactgccc
     8881 ggaacttgcc ggattcgttc ttgaagacat agtcggccgc gtcgtcccgg atcatctcct
     8941 tgttggtcgg cacgacgacc acttcgaggc tgtaaattcg atggaactct tcgtcttcgg
     9001 tcttggcggt accggtcatg ccggccagct tcttgtacat gcggaagtag ttctggaacg
     9061 tgatcgtcgc catcgtcacg ttctggcggc ggacgcggac gccttccttg gcctcgattg
     9121 cctggtggag accctcgctg tatcggcggc ccaccatcat gcgaccagtg aactcgtcga
     9181 cgatg
//
LOCUS       DATAOZ010000203         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6398490, whole genome shotgun sequence.
ACCESSION   DATAOZ010000203 DATAOZ010000000
VERSION     DATAOZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6398490"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..948)
                     /locus_tag="VHR64_05420"
     CDS             complement(277..948)
                     /locus_tag="VHR64_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX3302301.1"
                     /translation="MLKVFNDFERDPALLVQFDAVANCYSASCVFADVQYRQNVMDCE
                     IKPAFRAKVVGQALTVQLSPGDLVDPLKALEMGRPGDIIVVDAGGDRQTSVCGGLMGG
                     LAKNRGIRAMVIDGAGRDIDELEEIGWPIWTRAITARGTHTMFSERKEELSINVPIQC
                     GGVLVNPGDFIIADTIGVVVIPLARATEVVAKAREQADREQRTREWVASGKTVEDLLK
                     EFGRI"
     gene            complement(976..1977)
                     /locus_tag="VHR64_05425"
     CDS             complement(976..1977)
                     /locus_tag="VHR64_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX3302302.1"
                     /translation="MARQPKVVIADHDYGNLDIERPILEAAGARVVALQAKSEEDLFE
                     EARDCDAIINQYARVGAATIDRMERCKVIARYGIGVDIVDVEAATRKGILVTNVRDYC
                     TDEVADHAIGLWLALARKLVQYNAATHNGIWQWQTGRPIYRLRGRTMGIVSFGKIGQA
                     IAQRAKVFGVSLIAYDPYLPRDVFLTKGVNRVGKDEILERSDYLMMQVPMTPETLHFL
                     GEAELSRMKQGSIIVNTGRGPTIDNRALYRALVRKQIAGAGLDDPEEEPAKRKRWDPS
                     GNPLFSLDNVIVTSHVAYYSEESIRLARETAACEVARILKGERPLNPVNEVGLASPS"
     gene            complement(1992..2486)
                     /locus_tag="VHR64_05430"
     CDS             complement(1992..2486)
                     /locus_tag="VHR64_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase family protein"
                     /protein_id="HEX3302303.1"
                     /translation="MTALTFSHIGITVPDLDRAVEFYSKAFGLYVIMGATEIHQDDSA
                     IGQMCDDVFGPGWGSFRIAHLATGDGVGIELFEFPKTRPEQKPFEYWRPGLFHFCLQD
                     EQLEERVKLIESLGGRQRMKQVRHYYPGQKPYRMVYCEDPFGNIVELYSHSYELTYSA
                     GAYV"
     gene            complement(2483..3490)
                     /locus_tag="VHR64_05435"
     CDS             complement(2483..3490)
                     /locus_tag="VHR64_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302304.1"
                     /translation="MKRLLASPVAGPAIATIAVALLVTITTDRFLTQGNLNNLSLQVS
                     ILALVAIGSTAVIIVGGIDLSPGSAIALLTMLLAMMLKFWGLGFWQSILLVLMAGAAI
                     GLVNGTITAYLRIPAFITTLAGLSAYRGMAFMFNNGSPVFEASDRLEPLFYDQILGVP
                     LPLIYIVVFFAAAHWLMRHTRLGRSIYAVGGNANAARLSGIQVRRVQLYAFVLAGFMA
                     AVAAILMAARLNSGSPNYGVGLELQAIAAAVIGGASLAGGRGDMVATLFGALTVTIVQ
                     NGLNLNAIPTSTQNIVIGAIIIVAVGIDMWRGELSHVVAWVVSRQQQTRPERHEGRKA
                     Q"
     gene            complement(3487..>4192)
                     /locus_tag="VHR64_05440"
     CDS             complement(3487..>4192)
                     /locus_tag="VHR64_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3302305.1"
                     /translation="TDRGLRNVSFTLHRGEVLGLGGMLGSGRSAIARALFGVETVVEG
                     EIRVRGAATRVRHPADAIAAGVALLTENRNLDGLFFNFTGTENITVASLNDFDHGLWL
                     DLKRERRVSSELIGRLQVNRAAESGAIDRLSGGNQQKILVARWLNTKAEIFILDEPTK
                     GIDVGTKVAIYRLINELTASGKGVILISSDDKELLAMSDRVAIIRRGTIARIADPGEV
                     TKADLLTGPEERYRAA"
BASE COUNT          829 a         1298 c         1273 g          792 t
ORIGIN      
        1 gacaggacca tgccggacaa ccactcggag ccggcaaacc gccaaaatca ccaaagcggt
       61 ggcgtccaca acgcagccgt agaatgccgg tcccctcccg gtcaccaaag cgtttccaca
      121 cggccaagac ccatagcgga cgcaagtcgt ggaagagttt ccggccgctt ggaggtgtca
      181 atccgacatg ctgcggccgg ctggaggttt acggcttgcg accaggaaag tgcagcaaag
      241 gaccgcctcg gcggtcgcaa ccattggggt tcatttctaa atgcgcccga actccttgag
      301 cagatcctcg acggtcttcc cacttgccac ccattcgcga gtcctttgct cgcgatccgc
      361 ttgctcgcga gccttcgcga ccacttccgt ggcccgcgcc agaggtatga cgaccacccc
      421 gatcgtgtcc gctataatga aatcgccggg gttgactaga acaccgccgc actggattgg
      481 cacattgatc gacagttcct ccttccgttc cgagaacatc gtatgggtgc cccgggcagt
      541 gatggctcgc gtccagatcg gccaaccgat ctcctcgagt tcgtcgatat cgcggccggc
      601 accgtcgatc accattgccc ggataccgcg gttcttggcg aggccaccca tcaggccgcc
      661 acaaaccgag gtctgacggt ccccaccggc atcaacaacg atgatgtcgc cgggcctgcc
      721 catttcgagg gccttcagcg ggtccaccaa gtccccgggc gagagctgga cagtgagggc
      781 ctggccaacc accttggcac ggaacgccgg tttgatctca cagtccatca cgttctgacg
      841 atactgcaca tcggcgaaga cacacgatgc actgtagcag ttggcgaccg cgtcgaactg
      901 gacgagcagt gcgggatcgc gctcgaaatc gttgaacacc ttcagcatcc tgacatcctc
      961 tgctggttta cctcattacg atggggaggc gagcccaact tcattgacgg gattgagcgg
     1021 tcgctccccc ttgaggatac gggcaacctc gcaggccgcg gtttcccggg ccagccggat
     1081 cgactcctcc gaatagtagg cgacgtgcga ggtgacgatg acgttgtcga gtgagaacaa
     1141 tggattgccg gacgggtccc agcgtttccg cttcgctggc tcctcctcgg gatcatcgag
     1201 gccggcgccg gcaatctgct tccggaccag agcacgatag agggcccggt tgtcaatggt
     1261 cggaccccgg ccggtgttga cgataatgct gccctgcttc atgcggctga gctccgcttc
     1321 gccaagaaag tgcaatgtct ccggagtcat cggcacctgc atcatgaggt agtccgaccg
     1381 ctccaggatt tcgtctttgc cgaccctgtt cacccccttg gtcagaaaga cgtcgcgggg
     1441 aagatatggg tcatatgcaa tcagactcac accgaaaact ttcgcccgct gggcaatcgc
     1501 ctggccaatc ttgccgaacg agacgatccc catggtgcga ccccgaagtc ggtagatagg
     1561 ccgaccggtc tgccactgcc agatcccgtt gtgggtcgcc gcattgtatt gaacaagctt
     1621 gcgggcgagg gcgagccaga ggccgatggc gtggtcggcg acctcgtcgg tgcagtaatc
     1681 gcgaacattc gtgacgagta tgcctttcct tgtagcagcc tcaacatcga cgatgtcgac
     1741 gccgattccg tagcgggcga tgaccttgca gcgctccatc cgatcgattg ttgcggctcc
     1801 gacccgcgca tattgattga tgatggcgtc gcaatcgcga gcctcctcga acagatcttc
     1861 ctcagatttc gcttgcaagg ccaccacacg ggcgccggct gcctcgagga tcggccgctc
     1921 gatgtcgagg ttgccataat cgtggtcggc gatcactact ttgggctggc gcgccatgcg
     1981 accgactcca ctcagacgta ggcgccggcc gagtaggtga gctcgtagga gtggctgtag
     2041 agctcaacga tattgccgaa cgggtcctcg caatagacca tgcgataagg tttctggccg
     2101 gggtagtagt ggcgcacctg cttcatccgc tggcgccctc cgagcgactc gatgagcttc
     2161 acacgctcct caagctgttc gtcctgcagg caaaagtgaa agagcccagg ccgccaatat
     2221 tcgaagggct tctgctcggg cctggtcttg gggaactcga acagctcgat accgacaccg
     2281 tcgccggtcg cgaggtgggc aatgcgaaag ctcccccagc caggcccaaa cacgtcatcg
     2341 cacatctgtc cgatggcact gtcgtcttga tgaatctccg tcgcgcccat gatcacgtaa
     2401 aggccaaatg ccttggaata gaactctacg gccctgtcga gatcagggac ggtgatgccg
     2461 atgtgggaga aagtgagagc ggtcattggg ctttccttcc ttcgtgtcgt tcaggtctcg
     2521 tctgttgttg ccgagagacc acccaggcaa cgacatggct gagttcgcca cgccacatgt
     2581 cgatccccac cgccactatt atgatcgcgc cgatgacgat gttctgggtc gacgtcggga
     2641 ttgcgttgag gttgagcccg ttctggacga ttgtgaccgt caacgcgccg aacagggtcg
     2701 ctaccatgtc gccgcggcct ccggcgaggc tggcgccgcc gatgaccgcc gcagcgatcg
     2761 cctgcagctc caaccccacc ccataattcg gcgacccgga attgaggcgt gccgccatca
     2821 gtatcgccgc gaccgcggcc atgaagccgg ccagaacgaa ggcgtagagt tgcactcggc
     2881 ggacttgaat gccggacaag cgcgccgcat tggcgttgcc gccaacggcg tagattgaac
     2941 gaccgaggcg tgtatgccgc atcagccaat gggccgcggc aaagaacacg acaatgtaga
     3001 tgagcggcag agggacgccg aggatctggt catagaagag cggctcaagc cggtcggatg
     3061 cctcgaagac tggcgagccg ttgttgaaca tgaatgccat gccccgatag gcgctcagtc
     3121 cggcgagtgt cgtgatgaag gcggggattc gcagataggc cgtgatcgtg ccgttgacca
     3181 gtccgatagc tgccccggcc atgaggacca gcagaatcga ttgccagaat ccaaggcccc
     3241 agaatttcag catcatggcg agcagcatcg tcaggagggc gatggccgat ccgggactga
     3301 gatcgatgcc gccaacgatg atcaccgcgg tcgagccgat ggccaccaaa gcgaggatgc
     3361 tcacttgcag ggaaagattg ttgaggttgc cctgggtcag gaatcgatcg gtggtgattg
     3421 tgacgagcag ggccactgcg atggtggcga tcgccggtcc ggcaacaggc gaggcaagca
     3481 gccgtttcat gccgcacggt acctctcttc ggggccggtc aagagatcag ccttggtgac
     3541 ctctccaggg tcggcgatgc gggcaatcgt cccacgtcgg atgatcgcca cccggtcgga
     3601 catcgcgagc agctccttgt cgtccgaact gatcaggatc acgcccttgc ccgaggctgt
     3661 cagctcgttg ataagccgat aaatcgccac cttggttccg acgtcgatcc ccttggtcgg
     3721 ctcgtcgagg atgaagatct ctgccttggt gttgagccag cgagcaacga ggatcttctg
     3781 ctggttgcca cccgaaaggc gatcgatcgc cccggactcg gccgctcggt tcacctgcag
     3841 ccgcccgatc agctcgctgc tgacgcggcg ttcacgcttg agatcgagcc acaggccatg
     3901 gtcgaagtcg ttgagactgg cgacggtgat gttctcggtt ccggtgaagt tgaaaaagag
     3961 cccgtccagg ttccggttct cggtcagcag cgcgaccccg gcggcgatcg catcggccgg
     4021 atggcggacg cgagtcgctg ctccgcgcac ccggatttcg ccctcgacga cggtttcaac
     4081 accgaacaag gcgcgagcga tggcgctccg gccagaaccg agcatgcccc cgaggccgag
     4141 cacctcgccc cgatgaagcg tgaagcttac gttacgaagg ccacggtcgg tc
//
LOCUS       DATAOZ010000204         6433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4031179, whole genome shotgun sequence.
ACCESSION   DATAOZ010000204 DATAOZ010000000
VERSION     DATAOZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4031179"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VHR64_05445"
     CDS             <1..306
                     /locus_tag="VHR64_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302306.1"
                     /translation="GGLLAILDSPIAGLVLGAVLAIGAAALLPCSCFVDWDAKLAMRA
                     HDSRHARHPPVTIVTRQRPSPVPHPGRRNHLMVVHPPSGHAARGTMAPAPIRFMPRR"
     gene            complement(290..637)
                     /locus_tag="VHR64_05450"
     CDS             complement(290..637)
                     /locus_tag="VHR64_05450"
                     /inference="COORDINATES: protein motif:HMM:NF039368.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6176 family protein"
                     /protein_id="HEX3302307.1"
                     /translation="MDHICVVFPILSGKTQAAREFHRELSQQRMADYDKSERRIGITK
                     EDWFIANVNGGEQLVAYMEANDFNKALTKFSQSRDSFDMWFKARLADVTGVDLNNPPP
                     DMKFPELVSTYEA"
     gene            845..1297
                     /locus_tag="VHR64_05455"
     CDS             845..1297
                     /locus_tag="VHR64_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302308.1"
                     /translation="MSETGQLEFTEAEITRQALVHSSSLTYLMVAYARECGHSPEHVA
                     DFAGRIFTKGWARHVDEDALGIIRSIALIIVSIGGEVASIAGDSLEAEVRATGVVTEE
                     EAQFFGITRDEADRFCNVFVPMAASLGYVFNWRRDGNELVYTVRRAAF"
     gene            complement(1398..1772)
                     /locus_tag="VHR64_05460"
     CDS             complement(1398..1772)
                     /locus_tag="VHR64_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX3302309.1"
                     /translation="MSGASVEKKPSPDARASAHLSNEQTFLAWLRTGFNLIAVGLAAA
                     QFLKKDLVSSFPLTTVFSSVLVIGGIGLVILGSIRYREASQQIDDEAFKPTLQIATVG
                     TAIAIISGVLALAFVILLHERG"
     gene            complement(1769..3085)
                     /locus_tag="VHR64_05465"
     CDS             complement(1769..3085)
                     /locus_tag="VHR64_05465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302310.1"
                     /translation="MATRATAGRTPLINQIRQHVSAEDTRALTFGLRINIYGLGLMAL
                     WNTLNTVILPLRVDETAPASLRGSALGLVGLLGVGIAALIQPLAGRASDAATLPDRRR
                     PFIAVGTIVALPCLLVFGWAPTFALLLGAYIALQVATNIAQAAFQAFIPDLVGERDTG
                     IASGAKNLLTVAGAATGLIGIRVLQLLDLGIGVQLTFLAAILAVTAGLTMIWVPKISP
                     LPADKRQGGMSSALNPAILWRSFRKTFRQHATFRWAVVAQFLFLFGTYPAQRFLLYYL
                     RDRFGRGAEERASIGLLVAILIAAVAAVVAGAVSDRIGRRPVLYACLIGAGAGMVGVG
                     FAPTLWMVAIPGAILAGSVGAFQAVNWALLSDHVPKHQAATAFGLANVATAGAGALSG
                     VFGILVDVLNGFLGAGTWRITFTLAALIAVSAYFPLRRISPKDADA"
     gene            3268..3789
                     /locus_tag="VHR64_05470"
     CDS             3268..3789
                     /locus_tag="VHR64_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX3302311.1"
                     /translation="MFRSPLFNDFVSLRDTVDRLFNESFNDDQYRTSWSRSGNGRTQA
                     MPMALDVYATDDQVVILAAVPGMRPEDLDLTIHENTITISGKIANVAESNETKGATWY
                     VHELWSGDVRRSLTLPFPVDSDRADARFEQGILRVALPKAASAKPRKIKIGGGTEQGA
                     LTAGETPGEGSDE"
     gene            complement(3944..4792)
                     /locus_tag="VHR64_05475"
     CDS             complement(3944..4792)
                     /locus_tag="VHR64_05475"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3302312.1"
                     /translation="MSHGITNHLLGYPADARLLIINADDFGICHAVNEAIIGTIDDGL
                     VASTSLMTPCPWAPHAIQLLADRPEQPFGVHLTMVNDFGPYRWGPRASRDRVPLLVDE
                     TGYFYRYERIPELLAGARLEELELEFRAQIETVLAAGLRPTHLDWHCLADGGRPDIFD
                     LSFDLAREYGLAMRTHEREHAERCQRAGLPSIDHPVLDSYHMDPAEKPARYAQLLREL
                     PAGLTEWAVHPSLGDAEAQALEPENWRIRRADYDVFTSQETRDIIDEEGIILLDYRAF
                     QPLWVR"
     gene            complement(4989..5282)
                     /locus_tag="VHR64_05480"
     CDS             complement(4989..5282)
                     /locus_tag="VHR64_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302313.1"
                     /translation="MYVRIVHPTIPADKVEAAAKHWASFMSERARSNPMLERGFMAAT
                     ADRSSVVAVTIWKQLPDPDASQKMREEVMAQMQQFASGGMPPMDEFEVLAEIK"
     gene            complement(5304..5855)
                     /locus_tag="VHR64_05485"
     CDS             complement(5304..5855)
                     /locus_tag="VHR64_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302314.1"
                     /translation="MRRVPVLVLVMLVSLAGIIAFGRAAPTRAQQATPIAASQSPLIG
                     TWQLDTDTEDPSNPPEIAVFNADGSYLEVATDGAGPGRWESTGGDSANLNIWFLNTDD
                     HGNYTGTTIVRALVHVAADGQSFDADYTLELMMPDGSMSGQYGPGHAHGTLLSVEPMG
                     GPVGPLSDLFQQSEGTPEASPTP"
     gene            6026..6316
                     /locus_tag="VHR64_05490"
     CDS             6026..6316
                     /locus_tag="VHR64_05490"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX3302315.1"
                     /translation="MAKYVLLLTWTDLGIKQMHHTTERAKRVRQIVEELGGSEESVLW
                     TDGRYDIIATYDLPDEQAATVLDMRINALGTVRTEMMRGRTAGEITELLNKV"
BASE COUNT         1150 a         1942 c         2220 g         1121 t
ORIGIN      
        1 ggcggactcc ttgccatcct ggacagcccg atcgctgggt tggtgcttgg cgcggtgctg
       61 gcgatcgggg ccgcggcctt gctgccctgc agctgcttcg tggactggga cgccaagctg
      121 gcgatgcgcg cgcacgactc caggcacgct cggcacccgc ccgtcacgat cgtcacacgg
      181 cagcgtccgt cacccgtacc ccatccgggc cgaaggaatc atctgatggt cgtgcatccg
      241 cccagcggac acgccgccag ggggacaatg gcgccggcgc cgatccggtt catgcctcgt
      301 aggtagagac caattcgggg aacttcatgt ccggcggcgg gttgttcagg tcgacaccgg
      361 tgacatcggc gaggcgcgcc ttgaaccaca tgtcgaatga gtcccgggac tgcgagaact
      421 tcgtgagcgc cttgttgaag tcattcgctt ccatgtaggc cacgagctgc tctccgccat
      481 tgacgttggc aatgaaccag tcctctttgg tgataccgat ccggcgctcc gacttatcgt
      541 agtcggccat gcgttgctgg ctgagctcgc ggtggaattc gcgtgcggcc tgggtcttgc
      601 cggacaggat agggaagacg acgcagatgt gatccacggt actgcctcca gacggattcc
      661 gaatcgacac attcattcgt catgcgaagg gtttcatcct atcgcagcgt gccctgatgt
      721 caatctggct tattcgagcg gaatcgttcc cggccgccga ttggttcccg cattccttga
      781 cttatcgatg caattcgcga gaattgtgca catcgttcgt cgcgtcaaat tgacagggag
      841 tcacgtgtcc gaaaccgggc aactcgagtt cactgaagcc gaaatcaccc gccaggcttt
      901 ggtgcactcg tcctcactta cctacctgat ggtggcctac gcgcgggaat gcggccacag
      961 cccggagcac gtcgccgact tcgctggccg catcttcacc aagggatggg cgcgccacgt
     1021 cgacgaggat gccctcggca ttatccgcag catcgcgctg atcatcgtct cgatcggcgg
     1081 agaggtcgcc agcatcgctg gtgacagcct cgaggccgaa gtgcgcgcca ccggcgtggt
     1141 gactgaagag gaagcgcaat tcttcggtat cacgcgggac gaagctgatc gtttctgcaa
     1201 cgttttcgtc cccatggccg cgtcgctcgg ctacgtcttc aactggcgcc gcgacggcaa
     1261 cgaactggtg tacacggtgc ggcgggcggc gttctgaacc ggttttcggg attcggtttt
     1321 cggtgttcgg gattgtgctg ccgacggttc acctctgatc cctgatacct gacccctact
     1381 acccccaccc tcaaccctca accccgctca tggagcagga tcacgaaggc gagggcgagg
     1441 acgccactga tgatggcgat cgctgtgccg actgtggcga tctgaagcgt gggcttgaac
     1501 gcctcatcgt cgatctgctg gctggcttcc cgatagcgga tcgacccgag gatgacgagg
     1561 ccgatcccgc cgatcaccag gaccgatgag aagacagttg tcagcggaaa gctggagacc
     1621 aggtccttct tgaggaactg cgcggcggcc aggccgactg cgatcagatt gaacccggtc
     1681 cgcagccagg caaggaacgt ctgctcattc gagagatgcg cgctggcccg ggcatcggga
     1741 ctgggctttt tctccacgct cgctccgctc atgcatcggc atccttcggc gaaatccggc
     1801 gcaatgggaa atacgccgaa accgcgatca gcgcggccag cgtgaaggtg atccgccagg
     1861 tgccggcgcc gagaaacccg ttcagcacat cgaccaggat gccgaacacg ccggagagcg
     1921 cgccggcacc ggccgtcgcg acattggcga gaccgaaggc tgtggccgcc tggtgcttcg
     1981 gcacgtgatc gctgagcagg gcccagttga ccgcctggaa cgcgccgacc gagccggcca
     2041 ggatcgcgcc gggaatcgcg accatccaaa gcgtcggcgc gaaaccgacg ccgaccatgc
     2101 ccgcgccagc cccgatcagg caggcgtaga ggaccgggcg gcgaccgatc cggtcggata
     2161 cggcgccggc gacgacggct gccacggccg cgatcaggat cgcgacgagc aggccgatcg
     2221 atgcgcgctc ctcggctccc ctgccgaagc ggtcccgcaa gtagtagagc aggaagcgct
     2281 gggccgggta ggtaccgaag aggaagagga actgggcgac cactgcccag cggaaggtcg
     2341 cgtgctggcg gaatgtcttc cggaacgagc gccaaaggat ggccggattg agcgccgagc
     2401 tcatgccgcc ctggcgcttg tcggcgggca agggcgagat cttcgggacc cagatcatgg
     2461 tcagcccggc ggtgacggcg aggatcgcgg ccaggaaagt gagctgcacc ccgatcccga
     2521 gatcgagcag ctgcagaacg cggattccga tcaggccggt cgccgcgccg gcgaccgtca
     2581 acaggttctt ggcgccggag gcgatgccgg tatctcgctc gccgacgagg tccgggatga
     2641 aggcttggaa ggcggcctgg gcgatgttgg tcgcgacctg caaggcgatg tacgcgccga
     2701 gcaggagcgc gaaggtcggg gcccagccga agacgagcag gcagggcagg gccacgatcg
     2761 taccgacggc gatgaaggga cggcgccggt ccggcagggt cgcggcatcg ctagcccgac
     2821 cggccagggg ctggatcagg gccgcgatcc cgacgccgag caggccgacg aggccgagcg
     2881 cgctgccccg gagtgaggcg ggagcggttt cgtccacccg aagcgggagg atgactgtgt
     2941 tgagcgtgtt ccagagggcc atcagtccga ggccgtagat gttgatccgg aggccgaacg
     3001 tgagagcgcg ggtatcctcg gctgagacgt gctgtcgaat ctggttgatc agcggggtcc
     3061 tgcccgcggt cgctcgggtc gccatatcga gcagagtgtg gcgggttgca tctggagcaa
     3121 caaaacgtgg cgctacgatc aaccttgata cgtgtagctc atggtacttg acgtcggcaa
     3181 ggcaagaacc tacgatatat gtggttgctc gaccggatgg gtcggcgctg tacatggcca
     3241 gatacatgac ggaagaggtg atgaacgatg tttcgcagtc cgctctttaa tgatttcgtt
     3301 tccctccgcg atacggtcga tcggctcttc aacgagagct tcaacgacga ccagtaccgc
     3361 acctcgtggt cgcgctcggg taacgggcgc acgcaggcga tgccgatggc gctcgatgtc
     3421 tacgcgaccg acgatcaagt ggtgatcctg gcggcggtgc cggggatgcg gccggaagac
     3481 ctcgacctga cgatccatga gaacacgatc acgatcagcg gcaagatcgc gaatgtggcc
     3541 gagagcaatg aaaccaaggg cgcgacctgg tacgtccatg agctctggag cggcgatgtc
     3601 cggcgctcgc taacgctgcc attcccggtc gatagcgatc gggcggatgc gcggttcgag
     3661 cagggcatcc tgcgggtggc cctgccgaag gcagcgagcg ccaagccgcg caagatcaag
     3721 atcggcggcg gcacggagca gggcgcgctg acggccggcg agacgccggg cgaaggctcg
     3781 gacgagtaac cgctcgccgg atatcgaatc ttcatagagc ccgggtggtt tcacccgggc
     3841 tcatttcgtg tcgctttcgt agtgccggac ggctcgcccg gcttcggcga tcggttaccg
     3901 ggcgggccgc cccaacgtgt tgattgggat tcctctgccc gttctaccgg acccagagag
     3961 gctggaaggc tcggtagtcg aggaggatga tgccctcctc atcgatgatg tcgcgtgtct
     4021 cctgcgacgt gaagacatcg taatcggccc ggcggatccg ccagttttcg ggttcgaggg
     4081 cctgggcttc ggcgtcgccg aggctgggat gcacggccca ctccgtcaga ccggccggca
     4141 gctcccgcag aagctgggca taccgggccg gcttctcggc ggggtccatg tggtaactgt
     4201 ccagcacggg atgatcgatc gacggcaacc cggcgcgctg gcagcgctct gcgtgctccc
     4261 gctcgtgcgt gcgcatggcc agaccgtatt cccgcgccag gtcgaagctg aggtcgaaga
     4321 tgtcggggcg gccgccatcg gccaggcagt gccagtccag atgcgtcggg cggaggccgg
     4381 cggccaggac agtctcgatc tgggccctga actcgagctc gagctcctcc agcctggcgc
     4441 cggcgagcag ctccgggatc cgctcgtatc ggtagaagta gccggtctcg tcgaccagta
     4501 aggggacccg atctcgggag gcgcgcggac cccagcggta gggaccgaag tcgttgacca
     4561 tggtcaggtg cacgccgaag ggttgctccg ggcgatccgc gaggagctgg atcgcgtgcg
     4621 gggcccacgg gcagggcgtc atcaggctcg tcgaggcgac gagtccgtca tcgatcgtgc
     4681 cgatgatcgc ctcgttgacg gcgtggcaga tgccgaagtc gtcggcgttg atgatcagca
     4741 ggcgggcgtc ggccgggtag ccgagcaggt ggttcgtgat gccgtggctc aatcagcttg
     4801 cctcatcgag gatcggggtc gaatccaggg atttatacga tgcgcggctc agaaatgatg
     4861 aaggttgccg tcgggaatcg gcaaccggtc gtcctgggcc gcctcggtgt cggggacgcg
     4921 gcggaggaga acggaccgga tccatccgcc gcgtgttgtt gacccgagcc ggccacaggg
     4981 ggccggggct acttgatttc ggcgaggact tcgaactcgt ccatgggagg catgccgccg
     5041 gaggcgaact gctgcatctg ggccatgacc tcctcccgca tcttctgtga ggcgtccgga
     5101 tcgggcaatt gcttccagat cgtgacggcg acgaccgatg accgatcggc ggttgcggcc
     5161 atgaagcctc gctccagcat cgggttgctc ctggcccgct cgctcatgaa cgatgcccag
     5221 tgcttcgctg ctgcctcgac cttgtcggcc gggatcgttg ggtggacgat tctaacgtac
     5281 atggttgctc tcctttgtcc acgttacggg gtgggcgatg cctcgggcgt gccttcagac
     5341 tgctggaaca ggtctgagag cgggccgacc gggccaccca tcggctcgac cgagaggagc
     5401 gtgccgtggg cgtgaccggg tccgtactga ccggacatcg agccatcggg catcatcagc
     5461 tcgagcgtat agtcggcgtc gaagctctgg ccgtccgcgg caacgtggac cagggcgcga
     5521 acgatagtcg tcccggtgta attgccgtgg tcatccgtat tgaggaacca gatgttgaga
     5581 ttggccgagt cgcctcccgt gctctcccag cggccggggc cggcgccgtc ggtcgcaacc
     5641 tcgaggtacg agccatccgc attgaagacc gcgatctcgg gcgggttgct cggatcttcg
     5701 gtatcggtgt cgagttgcca ggtgccgatc agcgggctct ggcttgcggc gattggcgtc
     5761 gcctgttggg cccgggttgg ggcggcgcgg ccgaacgcga tgataccggc cagcgaaacg
     5821 agcatgacca ggacgaggac cgggacgcga cgcatgacga cctcctctcg cgggtccaga
     5881 tacggaccca tatccggctg aatacaccgt ttgctccata catagcctat gcctgctggt
     5941 caagggttcc catgaggccc ttgcgggcgg gtgtagcgta ggaacgagta cgaaacggtc
     6001 aatatcgcgg tgaggagatt tcaccatggc gaagtacgtg ctgctgctga cctggaccga
     6061 tttgggcatc aagcagatgc accacacgac agagcgggcg aagcgagtcc gccagatcgt
     6121 cgaggagctg ggcggctcgg aggagagcgt gctctggacc gacgggcggt acgacatcat
     6181 cgcgacttac gacctgccag atgagcaggc agcgaccgtg ctggacatgc ggatcaacgc
     6241 gctggggacg gtccggaccg agatgatgcg cgggcggacg gcgggcgaga tcaccgagct
     6301 actgaacaag gtgtgattga ggcgaggagg cgagcaggcg agcagtgatt acggcccgac
     6361 cactgacgtt tgggtcgcga agcgctaatc ggctcgcatg gtccagacct tcgtttcgcc
     6421 atcgggcgcg agc
//
LOCUS       DATAOZ010000205         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5610964, whole genome shotgun sequence.
ACCESSION   DATAOZ010000205 DATAOZ010000000
VERSION     DATAOZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5610964"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..123
                     /locus_tag="VHR64_05495"
     CDS             10..123
                     /locus_tag="VHR64_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302316.1"
                     /translation="MVMVKYGVNDIRAFAGNDLRFLRQFARADIGLGVVRA"
     gene            120..2561
                     /gene="pheT"
                     /locus_tag="VHR64_05500"
     CDS             120..2561
                     /gene="pheT"
                     /locus_tag="VHR64_05500"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX3302317.1"
                     /translation="MKVPMKWLAEFVQTDLSAQELAHRLTMAGLEAEKITQIGEMWGD
                     KVFVAEVRNVEKHPDADRLVLADVQAGDHRLTVVTGAPNIAAGQKVVLALTGARLFDG
                     YSDSPTPEIKTLKPGVIRGVKSEGMVCSEKELGISDEHEGILVLPDDAPVGMPFADYF
                     GDTVIEFEITPNLAHAFSINGIAREVHALLDVPVNRPPIFNLSLLADAPDDTVVIADP
                     ERCNRYMVLLVDNLVVGPSPAWMVQRLEAAGMRSINNIVDMTNYVMHEFGFPMHAFDR
                     DRLEGGRVIVRAAEPGEHLETLDHVERQLNPAMTVIADLEKPVGMAGIMGGFDAEVSD
                     ATTQLYLEVAHFNPTVTRATSRALKLRTDASARYERGLDPEGLPTAVARAAQLISELC
                     PGAVFVGLADAYPVPVERRTISFPFERIERLLGMRIDERQVLDILARLDFEAAISGGT
                     LTATVPTYRRDVLDREDIIEEVARIAGYELLPATLPSGEPQHVHRDPMYRLRKAARSV
                     LVGAGYNEAVTYVTIGRQDIDRFADKDSVGLVINSCRGHLIELRNALQAERNLMRPTL
                     IPSLLDVLSANLKHESGVRLAEIARVYAPGPERVLPDEVEVAGIVAAGQAEPLGLNAD
                     TDQIEFFDLKGMITLLLDRLGVQNASTSRWTHPAFHPGRAAQITVGGNVIARFGELHP
                     DTAAAYGINDVRVLAGEVNLTAALGVIEPRGRDAVVPRFLPVQQDFAVVVRNDVVASD
                     VESAFRSAAGPLLTDITLFDQFRGPQIGEGNVSMAWRLTFTAPDRTLTDNDLVKIRPK
                     IEKVLKQRVGGALRV"
     gene            2554..>3594
                     /gene="hisD"
                     /locus_tag="VHR64_05505"
     CDS             2554..>3594
                     /gene="hisD"
                     /locus_tag="VHR64_05505"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX3302318.1"
                     /translation="MSDVIRILTDLDQIREALTRSRGFEEPELSPQAKAGVKRVFGED
                     LPAGVVVDRILERVRTEGDVGVAALSAAIDGQAPYKVEVPRGEWEAAFDHIDPALQNA
                     MHVAAKQIEAFHRHQVRTSWFETSDEGILGQLVRPIERVGIYAPGGTAVYPSSLLMTA
                     VPARVAGVEEVVVCSPPHQGAISDLILAAAHVAGVDRVFQIGGAQAIAAMAYGTETVP
                     HVDKIYGPGNIFVVLAKQKLYGVAAIDALPGPTETLIVADETADPTLAAADLLAQAEH
                     DPMASAILLTDSAVLAEGVTAEVKRQLGELERKSIAGQSLKDNGLIGVVPDFRTAVDL
                     ANAYAPEHLCLLT"
BASE COUNT          647 a         1125 c         1172 g          650 t
ORIGIN      
        1 gtcgagcggc tggtcatggt caagtacggt gttaatgata tccgcgcctt cgcaggcaac
       61 gacctgcgct tcctgcgcca atttgcgcgc gctgatatcg gcctgggagt ggtccgcgca
      121 tgaaggttcc gatgaagtgg ctagctgagt tcgttcagac ggatctcagc gcacaagaat
      181 tggcccaccg cctcaccatg gcaggtctcg aggccgagaa gattacgcag atcggcgaga
      241 tgtggggcga caaggtcttc gtcgccgaag tccgcaacgt cgagaagcac cccgacgcgg
      301 accggctggt cctggccgac gtccaggccg gcgatcatcg cctgaccgtc gtcaccggtg
      361 caccgaacat tgcggctggg cagaaggtcg tgcttgcgct caccggcgct cgcctcttcg
      421 acggctactc ggacagcccg acgccggaga tcaagacgct aaagccgggc gtgatccgcg
      481 gcgttaagtc ggaagggatg gtctgctccg aaaaggagct cggcatctcg gacgagcatg
      541 agggcatcct ggtgcttccg gacgacgcac cggtcggtat gccgtttgcg gattacttcg
      601 gtgatacggt catcgaattc gagatcacgc cgaaccttgc gcatgcattc tcgatcaacg
      661 ggatcgcgcg cgaggtccac gccctgctcg acgtcccggt caatcgaccg ccgatattca
      721 atctctcgct gcttgccgac gctccagatg acacggtggt catcgccgac ccggagcgtt
      781 gcaaccgata catggtgctg ctggtcgaca accttgtcgt cggaccgtcg cccgcctgga
      841 tggtccagcg cctcgaggcg gccggaatgc gatcgatcaa caacatcgtg gacatgacga
      901 actacgtgat gcacgagttc ggattcccga tgcacgcctt cgaccgcgac cggctggaag
      961 gtggccgagt gatcgtgcgg gcagccgaac ccggcgagca cctcgagacg cttgatcacg
     1021 tcgaacggca gttgaatccg gcgatgacag tcatcgctga ccttgaaaag ccagttggca
     1081 tggcggggat catgggcggt tttgacgccg aggtgtcgga cgccacgacg cagctctacc
     1141 tcgaggtcgc gcacttcaac ccgacggtca cccgggcaac atcgcgcgcg ctcaagttgc
     1201 gcacggatgc cagtgcgcgg tacgagcggg gactcgatcc tgaggggttg ccgactgcag
     1261 tggcccgtgc cgcacagctg atctcggagc tctgtccggg cgcggtgttc gttggactgg
     1321 ccgacgcgta tccggtgccg gtcgagcggc gcacgatctc gttcccgttc gagcgcatcg
     1381 agcggcttct cggcatgcgc atcgatgagc ggcaggtcct ggacattctc gctcgactcg
     1441 atttcgaggc tgcgatctcc ggtgggaccc tgaccgcgac tgtgccgacc taccggcgcg
     1501 acgtgctcga tcgagaggac atcatcgaag aggtcgcccg catcgccggg tacgagctgc
     1561 tgcctgccac gctgccatcc ggcgagccgc aacacgtcca ccgcgacccg atgtaccgat
     1621 tgcgcaaggc agcgcgttcg gtgcttgtcg gtgccgggta caacgaagcg gtcacctacg
     1681 tcacgatcgg tcgccaggac atcgatcgat ttgctgacaa ggactcggtc ggcttggtca
     1741 tcaactcctg ccgcggtcat ctgatcgagc tcagaaacgc actccaggcc gagcgaaacc
     1801 tgatgcggcc gacgctgatt ccgtcgctgc ttgatgtact ctccgccaac ctgaagcacg
     1861 agtcgggagt tcgcctcgcc gagatcgctc gcgtatatgc gccaggcccc gaacgggtac
     1921 tcccggacga agttgaggtc gccgggatcg tcgcggccgg tcaagccgag ccactcggat
     1981 tgaacgcgga caccgatcaa atcgagttct tcgacctgaa ggggatgatc acgctcctac
     2041 tcgatcgact cggcgtgcag aacgccagca catcgaggtg gacgcatccg gcatttcatc
     2101 cgggtagagc cgcacagatc actgtcggtg gcaatgtcat cgcaagattc ggcgagcttc
     2161 acccggacac cgccgcggcg tatggaatca atgacgtgcg cgtcttggcc ggtgaggtca
     2221 acctgaccgc cgcgctgggc gtgattgagc cgcgtgggcg agatgccgtg gtgccgcggt
     2281 tcttgccggt tcagcaggac ttcgcggttg tcgtgcgcaa cgatgtcgtc gcatcggacg
     2341 tggaatccgc gttccggtcg gctgcaggtc ccctcctgac cgatatcacc ctcttcgatc
     2401 agtttcgcgg tccgcagatc ggcgagggca acgtcagcat ggcctggcgg cttaccttca
     2461 ctgccccgga ccggaccctg accgacaacg acctggtcaa gatcaggccg aagatcgaga
     2521 aggtgctcaa gcagcgggtt ggaggagcac tccgtgtctg acgtgattcg gatcctgacc
     2581 gatctcgacc agatccgaga agcgctgacc cgctcccgtg gctttgagga gccggagctg
     2641 tcgccgcagg cgaaggccgg ggtgaagcgc gtcttcggcg aggatttgcc agccggcgtc
     2701 gtggtggatc gaatcctcga gcgggtccgc accgagggcg acgttggagt tgcagctctg
     2761 agcgcggcaa tcgacgggca ggcgccttac aaggtcgaag tcccccgcgg cgagtgggag
     2821 gccgcctttg accacattga tcccgccctc cagaacgcga tgcacgtcgc cgcgaagcag
     2881 atcgaggcgt ttcaccggca tcaggtgcgg acctcgtggt tcgagacgag cgacgaaggc
     2941 atcctgggcc aactcgtgcg tccgatcgag cgagtcggca tctacgctcc cggtgggacc
     3001 gcggtctatc catcctcgct gctgatgacg gctgttccag cgcgagtggc cggggtcgag
     3061 gaggtagttg tctgctcgcc accgcaccag ggcgccatat ccgacctgat cctcgccgcg
     3121 gcgcatgtcg ctggggtgga tcgggtattt caaattggtg gtgcgcaggc gattgcggcg
     3181 atggcctatg gcaccgagac ggtgccacac gtcgacaaga tctacggccc cggcaacatc
     3241 ttcgtcgtgc tggccaagca gaagctgtac ggcgtggcgg cgatcgacgc cctgcccggc
     3301 ccaaccgaga cgctgatcgt cgcggacgag accgccgacc ccacgttggc cgccgcggat
     3361 ctgctggcgc aggcagagca cgatccgatg gcctccgcga tcctgctgac ggattcggca
     3421 gtcctggcgg agggcgtcac tgcggaggtc aagcggcaat taggggaact ggaacggaaa
     3481 tcgattgccg gtcagtcgct caaggacaac ggcctgatcg gtgttgtgcc tgatttccga
     3541 accgccgtgg atctcgcaaa cgcctacgcg ccggagcacc tctgcctgct gacc
//
LOCUS       DATAOZ010000206         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7454072, whole genome shotgun sequence.
ACCESSION   DATAOZ010000206 DATAOZ010000000
VERSION     DATAOZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7454072"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..992)
                     /locus_tag="VHR64_05510"
     CDS             complement(12..992)
                     /locus_tag="VHR64_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302319.1"
                     /translation="MDTIPVVELEDLKVKLGRREILHGITCRLGVSGTGKAVGLLGPN
                     GAGKSTLILTLLGFLKASGGRARILGMDCRHNMGEARSRIGYMPENDSFVGEMTAVTF
                     LRQMAELSGLPPKAALEKAHEVLFHVGLGESRYRELRTYSYGMKQMAKLAQAIVHGPE
                     LVVLDEPTNGLDPAARRRMLKLIVDMKEEQGMHVLVCSHLLRDIEQVCDEAVIMKDGA
                     IVHHCNLEEERRSNRSFVELEVTGDDRNLRMALPEIGADGVSEGGGRWRIVLPAGVEV
                     EAIWGLAARQNLLVRKLTHRRDTLEEIFLKAMGHLAQTPGESAALDAGRT"
     gene            complement(1105..>2475)
                     /locus_tag="VHR64_05515"
     CDS             complement(1105..>2475)
                     /locus_tag="VHR64_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302320.1"
                     /translation="GAWWNSETGQLLRQMTDRVQSVNSMLGDEIIFCASLSGVDGPMP
                     IVMAKVQGGKRAELSTALQGLFAAAGESPASYSVSDDLVVVSDSAPHLTWALSHLGQG
                     TGSPFAAAIAQRYQRGVGWLIGMDAPPLVTMASKDDAPPIDLAGMIGMKYVFLEQRAP
                     AGAVENEVTFAFQGARTGMGSWLADAGSGGAAEYLPAEALVAGYVSTREPLQLFEEFT
                     AQITRAAPDFERGLATMDEKLGAGFVQNLAGALGTEAALALTGLSTSGPTWVMASVAN
                     NPTVIDGSLQKLVETFNAELGPDEQGKRIALSQESAGGRTWNTMRPGGLPIGITWTYD
                     RGYIVAGSDRAVAERAIATRDAGSPLVWSPDFLGQLPSSAGLHPSAFGWLNPKGALGI
                     LSALTTNPAVSELVAGRDPILVVFDGTPEQIHAASRTRISGMIMDLMMIENLGRAQQS
                     DPVPRQ"
BASE COUNT          418 a          870 c          739 g          448 t
ORIGIN      
        1 acagcttctc gtcaggtgcg gccggcgtcg agcgccgcac tttcacccgg cgtctgcgcg
       61 aggtggccca tagctttcag gaagatctcc tccaacgtat cccggcggtg cgtgagcttt
      121 ctcaccagca ggttctgccg cgccgcgagc ccccagatgg cttcgacctc gacccccgcc
      181 ggcaggacga tacgccagcg cccgccgcct tcggacacgc catcggcgcc gatctccggc
      241 agcgccatcc gcaggttccg atcgtcgccg gtcacctcga gctcgacgaa ggacctgttc
      301 gaccgccgct cttcttcgag gttgcagtgg tgcacgatcg cgccgtcctt catgatcacg
      361 gcctcgtcgc acacctgctc gatgtcgcgc agcaggtggg agcagacgag cacgtgcatc
      421 ccctgctcct ccttcatatc gacgatgagc ttgagcatcc gccggcgcgc ggcaggatcg
      481 agcccgttgg tcggttcatc cagtaccacc agctccggcc cgtggacgat cgcctgggcg
      541 agcttggcca tctgcttcat gccgtacgaa taggtacgca actcccggta gcgcgactcg
      601 ccgagcccga catggaacaa cacctcgtgc gccttctcga gcgccgcctt gggcgggaga
      661 cccgacaatt ctgccatctg ccgcaagaac gtgaccgccg tcatctctcc gacgaacgag
      721 tcgttctcgg gcatgtagcc gatcctcgat cgggcctcgc ccatgttgtg ccggcagtcc
      781 atgccgagga tgcgcgcgcg tccgccgctc gccttgagga atccgaggag cgtcaggatc
      841 agcgtggact tgcccgctcc gttcggcccg agcaacccta cggcctttcc cgtgcccgaa
      901 acgccaagac ggcaggtgat cccgtgaagg atctcccgcc gtcccaactt gaccttcagg
      961 tcttcgagtt cgacaaccgg aattgtgtcc attcgtaatc gaggggcttc gctcctcgaa
     1021 ctcccctaca ctatcgctcg cggcgccccc aacttcccgc tccgctcccg tggctcactc
     1081 gctacgatcc ttcgcggcgc gcggtcactg tcgcggaacc gggtcggatt gctgcgcccg
     1141 gcccaggttc tcgatcatca tgaggtccat gatcatgccg gagatacgag tacggctggc
     1201 cgcatggatc tgttcgggcg ttccatcgaa caccacgaga atcggatcgc gcccggctac
     1261 gagttcgctg acggccgggt tggtcgtcag cgccgacagg attccgaggg cgcccttcgg
     1321 attcagccac ccgaatgcgg acgggtggag tccggcagac gacggcagct ggccgaggaa
     1381 gtcaggcgac cagacaagcg gtgagccggc atccctcgtc gcgatcgcac gctcggcgac
     1441 ggcgcgatcg gagccagcca cgatgtagcc gcgatcgtac gtccaagtga tgccgatcgg
     1501 aagtcctccc ggtctcatcg tgttccacgt ccgtccgccg gcgctctctt gcgacagtgc
     1561 gatgcgcttg ccctgctcgt caggtccaag ctcggcgttg aatgtttcca ccagcttctg
     1621 gagggaaccg tcgatgaccg tcggattgtt cgcgacgctc gccatgaccc acgtcgggcc
     1681 gctcgtcgag agtcccgtca gcgccagagc cgcctcggtg cccaacgcgc ccgccaggtt
     1741 ctggacgaag ccggcgccga gtttctcgtc catcgtcgcc aggccccgtt cgaagtccgg
     1801 cgccgccctg gtaatctgcg ccgtgaactc ttcgaatagc tgcagtggct cgcgggtcga
     1861 cacgtacccg gcgaccagag cctcggccgg caggtattcc gccgcgcccc cggagccggc
     1921 gtcggccagc catgacccca ttccggttcg tgcgccctga aacgcgaacg tgacctcgtt
     1981 ctcgactgca ccggctggag cacgctgctc gaggaagacg tacttcatcc cgatcattcc
     2041 ggcgagatcg atcggtggag cgtcatcttt ggacgccatc gtcaccaatg gaggcgcgtc
     2101 cattccgatc aaccagccga caccgcgctg gtatcgttgc gcaatggctg ccgcaaacgg
     2161 cgagccagtg ccctgcccca ggtgagagag agcccaggtc aagtgcggcg cagagtctga
     2221 aacaaccacc agatcgtcgg agaccgaata cgatgcggga gactcacccg ccgcggcgaa
     2281 cagtccttga agggcggtcg agagttccgc gcgcttgccg ccctgcacct tggccatcac
     2341 gatcggcatg ggaccgtcga cgccggaaag gctggcgcag aaaatgattt cgtcccccag
     2401 catcgagttc accgattgca cacggtcggt catctgtctc aacagctgtc ccgtttcaga
     2461 attccaccag gctcc
//
LOCUS       DATAOZ010000207         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6927342, whole genome shotgun sequence.
ACCESSION   DATAOZ010000207 DATAOZ010000000
VERSION     DATAOZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6927342"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VHR64_05520"
     CDS             complement(<1..243)
                     /locus_tag="VHR64_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEX3302321.1"
                     /translation="MNITLSPELETMIREKVEAGEYHDASEVISEALRVMAQNEKRTK
                     IRAMRDAALAQAIAEADRDETVELTNELWDQLMAEGD"
     gene            398..940
                     /locus_tag="VHR64_05525"
     CDS             398..940
                     /locus_tag="VHR64_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX3302322.1"
                     /translation="MFRGVAERSWMIVGQAPAAFGHERPAYAGPAGLKLRSWLTAAGL
                     EGDDPLEENFYLTSVTKCFPGPGIGGKGDRMPSRAEVLLCAPHLEREIQLVRPEVVVT
                     LGKLAANALIGPGSLSDLVGTVRNSSYAGHAFTAIPFPHPSGVSRWLNGEPGRSCLER
                     AIERLAEVRAVNPPGRAGRV"
     gene            1037..3451
                     /locus_tag="VHR64_05530"
     CDS             1037..3451
                     /locus_tag="VHR64_05530"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX3302323.1"
                     /translation="MSNHRTRLIPDLFGCGLLAVTIGLLFWNRATFDIWIARHDNLTA
                     YLPWWSYLGERLAAGQIPGWNPHQFSGIPFLADPQSGWMYVPTMLAFTIFDPLNGMKA
                     KLLIELLVAGYSTYALARLYGYSPIAAFCGAALFVFGPFSLFSAYCCTVRLHIATWIP
                     LALLGPEIALRSRRWPGRLAGIGFGAFAYSQMLAGWLGQGTYDAALIIGAYCLYRSLT
                     IGAIGSRFRRAATGIGIGLAVGLFGVALDAAALLPRLAFNGETHLGAGNYDQLTSGYY
                     YNPFGLRELVGSLLNDDYRHRGFTVPAAGILLALLAVALVPRAHPVPFFAGMTLVVYG
                     LTLDWGPPYWLLSLLPRWQELHNHYPQQVASAVMIGPAMLAAAAIDALPRLSRLRYRW
                     DRVLIPIAVIASGMIWVASNQDFNRPLRYPLFAVIIAALLTLVVATGRLGRAWTTRVL
                     VALALLVFIEPMGLEILDARTDGRIIAGWYDFWDPDPNLAAAARTAVLPDDPFGAGDF
                     LQQEKAAEGPFRYVGYGGIGYPGDPSGRITYMERRAEPQIQAILVNGRAIFLGLYDAQ
                     GYNPTQLDRYVEYVTAMNGHPIDYHLAELRPGGIGSPLLDMLNVRYILVDARLSPDRE
                     DVVAITQGERPVFENKDVRVYENPAAFPHVWLVHDVRDVSRAQADALLRDRAVDFRQV
                     ALVEGNAPEVALPDPRVAESATITRYQPERIEIAVTAAADGLLVVSDTYTTGWHATVD
                     GTSAPIYPTNLALRGIPVSQGTHTVVLTYSPPWLRPGIIISLLGHVLLLAAVAWWLHD
                     RRSRAH"
BASE COUNT          499 a         1191 c         1094 g          682 t
ORIGIN      
        1 gtcaccctct gccatcagct gatcccacag ctcgttcgtc agttcgaccg tttcgtcccg
       61 gtcggcctca gcgatagctt gggcaagggc ggcatctcgc attgcccgaa tcttcgttcg
      121 cttctcattc tgggccatga cccggagggc ttcgctgatc acctcactgg cgtcgtggta
      181 ctcgccggcc tcgacctttt cgcggatcat ggtttcgagt tcggggctga gcgtaatgtt
      241 catggatgtt acctgttaat tccgcatctt cgaaatttgc atctcacaga ttcgatacta
      301 gcaagaaatg ccagagatca atatcactca agtaggaacc caaataactg gttgccaagc
      361 gtgcgtctcg gctgggcaca ttgagatcgc tcggccagtg ttccggggtg tggccgagcg
      421 atcgtggatg atcgtcgggc aggcgccggc ggcgttcggg catgagcggc ccgcctatgc
      481 cggacccgcc gggctcaagc tgcgttcgtg gctgactgcc gccggcctgg agggcgacga
      541 cccgctcgag gagaatttct acctgaccag cgtcaccaag tgtttccccg ggccgggcat
      601 tggtggcaag ggcgaccgca tgccctcccg cgccgaggtt ctgctctgcg cgccgcacct
      661 cgagcgggag atccagctcg tgcggcccga ggtcgtcgtc acgctcggga agctggccgc
      721 taatgcgctg attgggccgg ggtcgctgtc cgatctcgtc ggcacggtcc ggaattccag
      781 ctacgccgga cacgcgttta cggcgattcc gtttccgcat ccgtccggtg tctcgcggtg
      841 gctgaatggc gaaccgggcc gctcctgcct ggaacgggcg atcgagcgcc tcgccgaagt
      901 ccgcgcagtg aatcctcctg gacgcgccgg tcgcgtgtag cgctgggcaa ttgcacactg
      961 cgccgatgcg tgtctaatcg gggctggagt gtcgcgaccg ggatgtgcgc cccactgatt
     1021 tgcgagagaa cgacgtgtgt ccaatcaccg cactcgcctg attccagatc tgttcggctg
     1081 cggcctcctc gccgtcacga tcggcctcct cttctggaac cgcgccacgt tcgacatctg
     1141 gatcgcgcgg catgacaacc tgaccgcgta tctgccctgg tggagctacc tcggcgagcg
     1201 gctcgcggcc gggcagatcc ccggctggaa cccgcaccag ttcagcggca ttcccttcct
     1261 ggccgacccg caatcgggct ggatgtacgt gccgacgatg ctcgccttca ccatcttcga
     1321 tccgctgaac gggatgaagg cgaagctgct gatcgagttg ctggtcgccg gatacagcac
     1381 ctacgcgctc gcccgcctct acggctacag cccgattgcc gccttctgcg gagcggcgct
     1441 cttcgtcttc ggtcccttct cgctcttctc ggcctactgc tgcacggtcc ggctccacat
     1501 cgcgacctgg atcccgctcg ccctgcttgg cccggagatc gcgctgcgct cccggaggtg
     1561 gccgggtcgg ctggccggga tcgggttcgg cgcgttcgcc tacagccaga tgctggccgg
     1621 gtggctcggg caagggacgt acgatgcggc cctgatcatc ggggcgtact gcctgtaccg
     1681 ctccctgacg atcggcgcga tcggcagccg attccgtcga gctgccactg gcattggaat
     1741 cggactggcg gtcggtctct tcggcgttgc gctcgatgcc gccgccttgc tgccccggct
     1801 ggccttcaat ggtgagacgc acctcggcgc cggcaattac gaccagctca cgagcggcta
     1861 ttactacaac ccgttcggcc ttcgagagct ggtcggttca ctgctcaacg acgactaccg
     1921 ccaccggggc ttcacggtcc cggccgccgg gatcctgctc gccctgcttg cggtcgcgct
     1981 cgtgccacgg gcgcacccgg tgcccttctt cgcggggatg acgctggttg tttatgggct
     2041 gacgctggac tggggacccc cttattggct gctctcgctg ctgccgaggt ggcaggagct
     2101 gcacaaccac tacccgcagc aggtggcctc cgcggtcatg atcgggccgg ccatgcttgc
     2161 cgcggcggcg atcgacgcgc tgccccggct ctcgcggctc aggtaccgct gggaccgggt
     2221 gctgatcccg atcgcggtca tcgccagcgg gatgatctgg gtggcatcca accaggactt
     2281 caaccgcccg ctccggtatc ccctgttcgc cgtgatcatc gccgccctgc tgaccctcgt
     2341 cgtcgcgacc ggtcgcctgg gtcgagcctg gactacgcgg gttctcgtcg cgctggcgct
     2401 gctcgtcttc attgagccga tgggactcga gatcctcgat gcccgcacgg atggccggat
     2461 catcgccggc tggtacgact tctgggatcc cgatccgaac cttgccgccg cggccaggac
     2521 cgccgtcttg ccagacgatc ccttcggcgc cggcgacttc ctgcagcagg agaaggcggc
     2581 tgaggggccg ttccgctacg tcgggtacgg cgggatcggc tacccgggcg atccgagcgg
     2641 ccggatcacc tacatggagc gccgggccga gccgcagatc caggcgatcc tggtcaacgg
     2701 ccgcgccatt ttcctcggcc tgtatgacgc gcaaggctat aacccgactc agcttgaccg
     2761 ctacgtcgag tacgtcacgg ccatgaacgg ccacccgatc gactaccacc tggccgagct
     2821 ccgccccggt gggatcggct cgccactgct cgatatgctc aatgtccggt acatcctggt
     2881 cgatgcccgc ttgtcgcccg accgggagga tgtcgtggca atcacccagg gggagcggcc
     2941 ggttttcgag aacaaggacg tccgcgtcta cgagaacccg gcggctttcc cgcatgtctg
     3001 gctggtccac gacgtccgcg acgtctcgcg ggctcaagcc gacgctctgc tgcgggaccg
     3061 ggcggtcgat ttcaggcagg tcgcgttggt cgagggcaac gcgcccgaag tcgccctgcc
     3121 tgatccgcgc gtggccgagt cggcgacgat tacccgatat cagcccgagc ggatcgagat
     3181 cgccgtgact gcggcggcgg acggtctcct cgtggtcagc gatacctaca ccacgggctg
     3241 gcacgcgacg gtcgatggca catcagcgcc aatctacccg accaatctcg cgctccgcgg
     3301 catcccggtc agccagggca cgcataccgt cgtcctgact tactccccgc cctggctccg
     3361 tcccgggatc atcatctcgc tcctgggtca tgtccttctg cttgccgctg ttgcctggtg
     3421 gctgcacgac cgccgctcca gggctcattg atttccccat cgcgcg
//
LOCUS       DATAOZ010000208         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1404301, whole genome shotgun sequence.
ACCESSION   DATAOZ010000208 DATAOZ010000000
VERSION     DATAOZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404301"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VHR64_05535"
     CDS             complement(<1..902)
                     /locus_tag="VHR64_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302324.1"
                     /translation="MPRRLEPHPHWNHLDTREELHAHATTTELTIGAARIRAGAVPLF
                     RASEGGLEQAIRLRVDGASGPVQLSVSHGQTTLDRTTVDLAATAQSVHLFVPEVESET
                     RFRLDVESGADRSSAEINVAPQRKWSIHLIHHSHFDYGYTDPQAVVMEHQLRYLDAAL
                     DLIRATDDWDADAAFRWNVEVTYPLQQWLRTRPRPMREEFVQRVKEGRIEVNALPFSM
                     HTEVYSIDELAWGLKFTDELREQYGIEIVSAIQSDVPGATIGLLNLLTSADIQYFSVA
                     HNYAGRSIPFRYGGQDLTRPFWWKA"
     gene            complement(945..1802)
                     /locus_tag="VHR64_05540"
     CDS             complement(945..1802)
                     /locus_tag="VHR64_05540"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3302325.1"
                     /translation="MKPLVAFERGSVFFDATLGPIRLKHPEGIAIASDGAIWCGGDGG
                     EIYRITPDGSGIELIASTNGFTLGMAFDTEGMLYTCDLGHKTVFRLNPATGTLQTFAA
                     GGDGATLRVPNYPVVDTTRNCMYVSDSYGAGTPGPGVWRFDLDTGKGALWYDRPLNFA
                     NGMALSPDRQTLYVIETFARRVTRIPIKHDGSAGDAETFVEGIERLPDGLAFDAEGNL
                     YISCYEPSRLFRATPDGQLELLFDDPEAHLLCHPTNCAFRGTDLFTSNLGRWHITRIE
                     LGVSGAPLL"
     gene            complement(1843..2574)
                     /locus_tag="VHR64_05545"
     CDS             complement(1843..2574)
                     /locus_tag="VHR64_05545"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX3302326.1"
                     /translation="MNTHVEIPTSSTPARPNLSTYLSQQILSLIRDRQLKPGDRLPSA
                     RALAAQFSVATPTIREALRRLQATGMIDIRHGSGIYVRRDADRILLSNPSYGALEAQT
                     ILQALDARLLIEPHLAELAAANASEGDIAGLRIILERSERALAHHDDERYIQINYELH
                     VGIAQASGNIVLTHIVQSLLEMHATELHIVDPHSSLSEIRARDHHFHQLVIAAIAEHD
                     ESAARDAMTEHLTVARSTIELRVAQ"
BASE COUNT          509 a          771 c          859 g          495 t
ORIGIN      
        1 gccttccacc agaagggccg ggtcaggtcc tgccctccgt agcgaaacgg aatcgatcgg
       61 ccggcgtagt tgtgcgcgac tgagaagtac tgaatatcgg ccgacgtcag gaggttgagc
      121 aggccgatcg tcgcgcccgg gacgtcggac tggatcgccg agacgatctc aatgccgtac
      181 tgctcgcgca gctcatcggt gaacttgagg ccccaggcca gctcatcgat cgagtagacc
      241 tcggtatgca tgctgaaggg cagggcattg acttcgatcc ggccctcctt gacccgctga
      301 acgaactcct cgcgcatcgg gcgaggtcgc gtccgcagcc actgctggag tggatatgtg
      361 acctcgacat tccaccggaa ggcggcatcc gcatcccagt cgtcggttgc gcggatcagg
      421 tcgagagcgg cgtcgaggta gcggagctga tgctccatga ccacggcctg tggatcggta
      481 tagccgtagt cgaaatgcga gtgatggatc aggtggatcg accacttgcg ctgcggagcg
      541 acgttaatct ctgcagagga tcggtcagcg cccgactcga catcgagccg gaaacgtgtc
      601 tcgctctcaa cctcgggcac gaagaggtgt actgactgcg ccgtagcggc gaggtcgact
      661 gtcgtccggt ccaacgtggt ctgcccgtgt gagactgaaa gctgcaccgg accgcttgcc
      721 ccgtcgacgc gcaaccgaat cgcctgctcg agcccacctt cgctggcccg gaacagcgga
      781 acagcacccg cgcgaatgcg ggctgcaccg atggtcagct cagtcgtggt cgcgtgggcg
      841 tggagctctt cgcgcgtgtc aagatggttc caatgcggat gcggctcaag acgacgaggc
      901 acagcagcga ttctccttca cggtccaacg atcaagaatt tcgactagag gagcggtgct
      961 cccgatactc caagttcgat cctggtgatg tgccagcggc cgaggttcga tgtaaagaga
     1021 tcagttcccc ggaacgcgca attggtcgga tggcacagca ggtgcgcctc cgggtcgtcg
     1081 aagagcaact cgagctggcc gtcgggtgtg gcgcggaaca ggcgagacgg ctcataacac
     1141 gagatgtaga gattgccctc ggcatcaaat gcgaggccgt ctggcagccg ctcgatcccc
     1201 tcgacgaacg tctcggcgtc accggcactg ccgtcgtgct tgatagggat gcgggtgaca
     1261 cggcgcgcga acgtctcgat gacataaagc gtctgccggt ccggcgagag cgccattccg
     1321 ttggcgaaat tgagcggacg gtcgtaccag agtgcgcctt tgcccgtgtc gaggtcaaat
     1381 cgccagactc ccggacccgg cgtgccggcg ccgtaactgt ccgagacata catgcagttg
     1441 cgagtcgtgt cgacgaccgg gtaattgggg acgcgtaacg ttgctccgtc gccaccggcc
     1501 gcaaatgtct gaagcgttcc ggtggcgggg ttgagccgaa aaacggtctt gtgcccaagg
     1561 tcgcaggtgt agagcatccc ctcggtgtcg aaggccatcc cgagcgtgaa gccattcgtc
     1621 gaagcgatca actcgattcc agacccgtcc ggcgtgatcc gatagatctc gccaccatcg
     1681 cctccgcacc agatcgcgcc gtcggaagcg atggcgattc cttccgggtg cttgagccgg
     1741 atcggcccga gggttgcatc aaagaagaca gacccgcgtt caaatgccac tagcggtttc
     1801 acggatgctc tcctgcgtgc ccgggatccc gttccaggtt gctcactgcg ccacgcgcaa
     1861 ctcaatcgtc gaccgggcaa ccgtgagatg ttcagtcatc gcatcgcgcg cggccgactc
     1921 gtcatgttcg gcgattgcgg caatcacgag ttgatggaag tggtggtcgc gcgcgcgaat
     1981 ctcggacagc gaactgtggg gatcgacaat gtgcaactcg gtggcgtgca tttcgagaag
     2041 tgactgaacg atatgggtca gcacgatatt gccggatgcc tgggcgattc cgacatggag
     2101 ctcgtagttg atctggatgt accgctcatc atcgtgatgc gcgagggccc gctcggaccg
     2161 ttccagaatt attcgaagac cggcaatgtc cccttcgctg gcattggcgg cggccaactc
     2221 ggccaagtgc ggctcgatca gcagccgggc gtcgagagcc tgcaagatcg tctgcgcttc
     2281 gagagcacca tacgacggat tcgagagcag aatgcgatct gcgtcccggc gaacgtagat
     2341 gccggatccg tgacggatat cgatcatgcc ggttgcctgc agccgtcgga gcgcttcgcg
     2401 aatcgtgggc gtggcgaccg agaattgggc cgcaagggcg cgcgccgatg gcaggcgatc
     2461 gccgggcttg agctggcggt cgcgaatcag ggacagaatc tgctgggaca gataggtcga
     2521 aagattgggg cgggccggcg tcgatgaggt cgggatttcc acgtgcgtgt tcactatcct
     2581 cgccgtgtcc ctgcgaacag cgcgattgat ctaatcattt agtgaatcta cgcg
//
LOCUS       DATAOZ010000209         7740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2194826, whole genome shotgun sequence.
ACCESSION   DATAOZ010000209 DATAOZ010000000
VERSION     DATAOZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194826"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VHR64_05550"
     CDS             complement(<1..747)
                     /locus_tag="VHR64_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302327.1"
                     /translation="MSRLLESSDVRLITFTGPGGVGKTRLALETVAGRESVGSVPVDV
                     VPLASIRDYQLVIRSIARAIGIPEQPGLSDVESLARGIGDGRRLIVLDNLEQVVDAGP
                     WLAELLVRCPGLTLVCTSRVRLNIQGESLIPVPPLALPPNDRHVSPDSARTVPAVALF
                     IQRARQIRPDFDLTDDNCADVIDLCRKLDGLPLAIELAAARLSLLSLGALRSGLDHRF
                     ELLAGGTRDNPPRLQSLSEAINWSYELLDPH"
     gene            complement(1010..1504)
                     /locus_tag="VHR64_05555"
     CDS             complement(1010..1504)
                     /locus_tag="VHR64_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3302328.1"
                     /translation="MRARQRLLVIIGALMVTFLGGYSIMAQNTPTPTATASPEASPGA
                     SPGASPQASPVGTPIGLPVGNAEEGQKLVTQCLGCHSVDGSILVGPSWKDLYGHEVEL
                     EDGTKVIADEAYIYNSIKNPTSQIVKGFPPAMPPYAYLTDQQIADIIAYIQTLSPEGE
                     EHDD"
     gene            complement(1517..1972)
                     /locus_tag="VHR64_05560"
     CDS             complement(1517..1972)
                     /locus_tag="VHR64_05560"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEX3302329.1"
                     /translation="MIRRRTFLLGVAAVGLGACGGDDTKGDDGPPKISYGSETCDRCN
                     MVIGEERHAAALKGADGNWLAFDDTGEMVLTAQDSTGSDYKAWVHDYETMAWHDAATA
                     FYVWLPNRSTPMATGIVAYGDQARATAKAQEIGGWSKSWAAMLADWTMN"
     gene            complement(1969..2799)
                     /locus_tag="VHR64_05565"
     CDS             complement(1969..2799)
                     /locus_tag="VHR64_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3302330.1"
                     /translation="MERSVIAAIAARDVRSGVRNRWFMLYAIVFIMLSVGFSMLAMGG
                     STLTGQPGFGRTAAGLLNLMLLMVPLIGLTIGAQSLVGERQDRSLDYLMAQPISTREI
                     FLGKYLGAAASLALLLLLGFGVSGVVMAVRGAATGLGDFLLLVLLTTLLGLAMLSAGY
                     LISSRSPQTAAALGIALTLWLLLVIVGDLGLMSSAIVMDLSPGTLLTLTLVNPLDVYK
                     LLGVDLLQTSLDVLGPAGIYANSQLGGVLTPLLAILLLAWIVAPLPVGYLLFTRKDVR
                     "
     gene            complement(2781..3683)
                     /locus_tag="VHR64_05570"
     CDS             complement(2781..3683)
                     /locus_tag="VHR64_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302331.1"
                     /translation="MTTQMTDSSGGISVRNVTKRYGQWKALDDVSFAVQPGESLGMWG
                     PNGAGKTTIMRCMLGIARYEGEVRIGGFDPARQGREARSLIGFVPQDLPVSPMSVSEM
                     GRFIAKLKGCPAGVEVEPLELLGLGDQGDKAVGALSGGMRQRLALALALIGTPQILLL
                     DEPTANLDARGRAELLDLLRRLKAQGLTLVFSSHRPEDVVSMADRVLMIEGGKVQTFL
                     PAAEFRREHESGTRLVITLTNGHLHEALTALERVGVPATGSGHVLAVDVHEARKAEVI
                     TLLAREGVDIADFEVERGKWNVQS"
     gene            complement(3680..5149)
                     /locus_tag="VHR64_05575"
     CDS             complement(3680..5149)
                     /locus_tag="VHR64_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEX3302332.1"
                     /translation="MRSRNNQPFIRRLGQGLAAVGIAVVLAGPAIPTPPAGAAELEVC
                     PTCTVTSLADAVALAEPGATITVRGGTYPGGMVITKSLTLVGVENPVIDGQEQGTLIS
                     ASGADLTISGFTLRSTGSNHDREDSAIVVENGKATITDNWIEDALFGIYLKTAHGSVI
                     RNNTILAKPVDIAMRGDGIKIWYCDDVLVEHNEASDGRDIILWYSNRGVVRGNDFNRE
                     RYGLHLMFSNDIRIENNSLSENSIGLFIMYSRNPLVIGNTMSNNHGPSGGGMGLKDVD
                     NAVVEGNRFVNNQVGAQIDTSPREMGIENYWKDNVFAFNHTAVALQPSVRHNTFTGNN
                     FIDNGQHVSIVGSGDLKDITWAVDGRGNYWSDYAGYDANGDGVGDVPYRSQRLFDSLL
                     DEQPSLRLFLFSPAAMAVDFAAKALPSVRPQTKLEDPHPLVNPVQSALLPDATTAGNR
                     TRVLTGATGLLFAIPAIVVVGWGRPKRGPRRSKRGASTP"
     gene            complement(5133..5795)
                     /locus_tag="VHR64_05580"
     CDS             complement(5133..5795)
                     /locus_tag="VHR64_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX3302333.1"
                     /translation="MVATHHNQAAQAVKPAAHGLSQVRIGLDARLARFFLQAGAGILL
                     VASMFYPYWEITLHAPQYPRGLFIQTFVNKMEPARNVFEVDGLNHYIGMIKLTDAATI
                     EQAISVYAIPAVALLATASFLVTGIWRTLLRLPVILYPVIFAVDLFSWLYYAGHTLDP
                     KAPMSSSIKEFTPKILGKGTIGQFHTEANFTTGFYLAAAAAVITLVVAIWERWSRHAQ
                     SE"
     gene            complement(5866..>7740)
                     /gene="nosZ"
                     /locus_tag="VHR64_05585"
     CDS             complement(5866..>7740)
                     /gene="nosZ"
                     /locus_tag="VHR64_05585"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HEX3302334.1"
                     /translation="AQAQSAEDIANARGLTPQDMTAALKTYVPSGKWDEYYIFASGGH
                     GGQVLVVGVPSMRLLKVIGVFTPEPWQGFGFDDGSRAMLEAGGMNGKTLTWADTHHPG
                     LSETNGEYDGQFLFINDKAHARMAVIDLRDFETKQIVKNPHIISNHGGAFVTPNTDYI
                     IDGSQYAAPFGWEYAPLSDYKTSYRGAMTYWKFNRETGRIDPSQSFSIEIPPYWQDLA
                     DAGKGPSDGWSFSNSLNTEMALGGIMDGQPPVEAGASMRDMDYLSVINWRKAAEVIAN
                     GGGEDVKGMKMIRMADAVSNGLIYQVPEPKSPHGVDVTPDGKYLVVAGKLDPHVTIYS
                     FQKIQDAIAAGGYSTDEFGVPVLDFATCVEAQVELGLGPLHTQFDNAGNAYTSLFLDS
                     AIAKWKLGGEGVEGGWTLIEKIPVNYNVGHLTAIEGDTVTPQGNFVIALNKWAIDRFA
                     PVGPLHPQNFQLIDITGETMQVLYDMPIGIGEPHYAQTIRASKLQPWVTYPEMGFDSL
                     EMKKKEGAPEPGKEGVIVEGNKVTINMTSVRSHFTPDNIEVPVGSEVTWNITNIEMAE
                     DATHGFALPGQNINLSIEPGESTTITFTADTAGVYPYYCTEFCSALHLEMMGYFLVKP
                     "
BASE COUNT         1346 a         2497 c         2491 g         1406 t
ORIGIN      
        1 gtgcgggtcg agcagctcgt agctccagtt gatcgcctcg ctgaggctct gcagcctggg
       61 cgggttgtcg cgcgtgccgc cggccagcag ctcgaagcgg tggtcgagcc cggatcggag
      121 cgcgccaagc gacagcagcg acagtcgagc ggctgccagc tcgatcgcca gcggcaaccc
      181 gtctagcttg cggcagaggt cgatgacatc ggcgcagttg tcgtcagtca aatcgaagtc
      241 gggccggatc tgccgggcgc gctggatgaa gagggcaacg gccggaactg tgcgggccga
      301 gtctggcgaa acatggcgat cgttcggcgg caaggcaaga ggcggcacgg gtatgagcga
      361 ctcgccctgg atattgagcc ggacccgact ggtgcagaca agggtgagcc cgggacaacg
      421 cacgagcaac tcggccaacc agggccccgc atcgaccact tgctcgaggt tgtcgagaac
      481 gatcagccgt cgaccatctc cgataccgcg tgccagtgac tcgacgtcgc tcaaccccgg
      541 ttgctcggga atgccgatcg cacgcgcgat gctccggatg accaactggt agtcgcggat
      601 agaggcaagc gggacgacat cgacgggcac actcccgacc gattcacggc ccgcgaccgt
      661 ctccagggca agccgcgtct tcccgactcc accgggaccg gtgaacgtga tcagccgaac
      721 gtcactggac tccaggagcc gggacaaggt cgcagattca gtttcgcgcc caacaaacga
      781 gctgatcggg cgcggtaccc ggccgatccg tcctgggctg ccttcatctg gcatggccgt
      841 tgcgcacatc gggccgaccc cacgcgtatg gtgtggcgag tagtgtttgc atggtaaatc
      901 gtcatgtcca gcatacaggt cacgacgtta cggacgggca cgcatggcgg gcacaagaaa
      961 ccgcccggtc gatatcgacc cggcgatttc tcgctgattc gattcggggc tagtcgtcgt
     1021 gttcctcgcc ctccggactg agcgtctgga tgtaggcgat gatgtcggcg atctgctgat
     1081 cggtcaggta ggcatagggc ggcatcgccg gcgggaagcc cttgacgatc tgcgaggtcg
     1141 ggttcttgat cgagttgtag atgtacgcct cgtcggcgat caccttggta ccgtcctcga
     1201 gctcgacctc gtgcccgtag aggtccttcc agctcgggcc aacgaggatg ctgccgtcaa
     1261 ctgagtggca gccaaggcat tgcgtgacca gcttctggcc ctcttcggcg ttgccaaccg
     1321 ggagcccaat cggcgtcccg acgggagaag cctggggtga ggcccctggc gatgcccccg
     1381 gcgaggcctc gggggaggcg gtcgcagttg gtgtgggcgt gttctgggcc ataatgctgt
     1441 agccgcccag aaacgtcacc atcaatgcgc caatgatcac caacagtcgt tgtcgtgccc
     1501 gcatcgtccc ctctccctag ttcatggtcc aatcggccaa catcgccgcc caggacttgc
     1561 tccaaccacc gatctcctgc gcctttgccg tagcccgtgc ttgatcaccg taggcgacga
     1621 tcccggtcgc catcggggtg ctgcggttcg gcagccagac gtagaaggcc gtagcagcat
     1681 catgccacgc cattgtctcg tagtcatgta cccaggcttt gtagtcgctg cccgtggaat
     1741 cctgcgcggt caggaccatt tcgcccgtgt catcgaaggc gagccaattg ccgtcggcac
     1801 ccttcagggc cgcggcatga cgctcctctc cgatcaccat gttgcagcga tcgcaggtct
     1861 cgctcccata tgagatcttc ggcggcccat cgtcaccctt cgtgtcgtct ccgccacacg
     1921 cgccgaggcc gaccgccgcg acaccgagca gaaacgtccg tcgcctgatc accgcacatc
     1981 cttccgtgtg aagagcaggt agccaaccgg cagcggcgcg acgatccagg ccaggagcag
     2041 gatcgccagc aggggagtca ggactccgcc gagttgcgag ttggcataga tcccggccgg
     2101 accaagcaca tcgagcgatg tctgcagcag gtccacgccg agcaatttgt agacatcgag
     2161 cgggttgacc agtgtcaggg tcagcagcgt gcccgggctg agatccatca cgatcgcgct
     2221 gctcatcagg ccgagatcgc cgacgatcac cagcaacagc cagagcgtca gcgcgatgcc
     2281 gagcgcggcg gccgtctgcg gcgatcgact cgagatcagg tagccggcgc tgagcatggc
     2341 caggccgagg agcgtcgtca gcaggacgag cagcaggaaa tcgccgagcc cggtcgccgc
     2401 gccgcgcacg gccatgacca cacccgagac accgaagccg agcaggagca gcagggccag
     2461 cgaggcggcc gcgccgaggt acttgccgag gaagatctcg cgggtcgaga tcggctgggc
     2521 catcagataa tcgaggctgc gatcctgccg ctcgccgacc aggctctgcg cgccgatggt
     2581 caggccgatc agcggcacca tcagcagcat caggttgagc aagccggccg cggtccgccc
     2641 gaagcccggc tgcccggtca gcgtcgatcc acccatcgcc agcatcgaga agccgacgct
     2701 gagcatgatg aagacgatcg cgtagagcat gaaccagcga ttgcggacac ccgagcggac
     2761 atcccgggcg gcgattgccg ctatgactga acgttccatt tgccccgctc cacttcaaag
     2821 tcggcgatat cgacgccctc gcgcgcgagc agggtgatca cctccgcctt gcgcgcctca
     2881 tgcacgtcga ccgccaggac atgcccggac ccggtcgctg gcacacccac gcgctccagc
     2941 gcggtcagcg cctcgtgcag gtggccgttc gtcagcgtga tcaccaggcg cgtgccactc
     3001 tcgtgctcgc gccggaactc ggctgccggc aggaaggtct ggaccttgcc gccctcgatc
     3061 atcaggaccc gatccgccat cgagacgacg tcctcgggcc ggtgcgagct gaaaaccagc
     3121 gtcagtccct gggccttgag gcggcggagc agatcgagca actcggcgcg gcccctggcg
     3181 tcgaggttgg ccgtcggctc atcgagcagc aggatctgcg gcgtgccgat cagggccagc
     3241 gccaaagcga gccgctgccg cataccgccc gatagcgcgc cgaccgcctt gtcaccctga
     3301 tcgccgagcc caagcagctc cagcggctcg acttccacgc cggccgggca acccttcagc
     3361 ttggcaataa agcggcccat ctcggagacc gacatcggcg agaccggcag atcctgcggt
     3421 acgaagccaa tcaggctgcg cgcctcgcgg ccctgcctcg cggggtcgaa tccgccgatc
     3481 cgaacctcac cctcgtaccg ggcgatgccg agcatgcagc gcatgatcgt cgtcttgccc
     3541 gcgccgtttg ggccccacat gccgagcgat tcgcccggct ggacggcgaa gctgacgtca
     3601 tcgagcgcct tccactggcc gtagcgtttg gtcacgttcc ggacggagat cccgccggag
     3661 ctgtcggtca tctgcgttgt catggcgtgc tcgctccccg tttcgatcgt cgtggacccc
     3721 gcttcggtcg accccacccg acgaccacga tcgccgggat ggcgaagagc agcccggtgg
     3781 cgccggtcag gacacgggtc cggttaccgg ccgtcgtcgc gtctggcagc agggcggatt
     3841 gcaccgggtt gaccagcgga tgcggatcct caagcttggt ttgcggtcgc acgctcggca
     3901 gcgccttggc cgcgaaatcg accgccatcg ccgctgggct gaacaggaac agccgcagtg
     3961 agggctgctc gtccagcagc gagtcgaaca ggcgctggct gcggtacggc acgtcaccga
     4021 ccccatcgcc attcgcgtcg tacccggcgt agtcgctcca gtaattgccg cggccgtcaa
     4081 ccgcccaggt gatgtccttc aggtcgccgc taccgacgat cgagacgtgc tgcccgttgt
     4141 cgatgaaatt gttgccggtg aaggtgttgt gccggacaga cggctgcagc gccacggcgg
     4201 tgtggttgaa tgcgaatacg ttgtctttcc agtagttctc gatgcccatc tcgcgcggcg
     4261 aggtgtcgat ctgcgcgccg acctggttgt tgacgaagcg gttgccttcg accaccgcgt
     4321 tgtcgacgtc cttgaggccc attccgccgc cgctcggccc atggttgttc gacatcgtgt
     4381 tgccgatcac gagcggattg cggctgtaca tgatgaagag gccgatcgag ttctcgctca
     4441 gcgagttgtt ttcgatgcga atatcattgc tgaacataag gtgaaggccg tagcgctccc
     4501 ggttgaagtc attgccccgg accacgccgc ggttcgagta ccagaggatg atgtcgcgcc
     4561 cgtcgctggc ctcgttgtgc tcgaccagga catcgtcgca ataccagatc ttgatgccgt
     4621 cgccgcgcat cgcgatgtcg accggcttgg ccaggatcgt gttgttccgg atcacgctgc
     4681 cgtgcgccgt cttgaggtag atgccgaaga gggcgtcctc gatccagttg tccgtaatcg
     4741 tggctttgcc gttctcgact acgatcgccg agtcctcgcg gtcgtggttc gagccggtcg
     4801 agcggagggt gaagccgctg atggtcagat cggcgccgga cgccgagatc agcgtgccct
     4861 gctcctgccc atcgatcacc ggattttcga ccccgaccag ggtcaggctc ttcgtaatga
     4921 ccatgccgcc ggggtaggtg cccccgcgga cggtgatcgt cgcgcccggc tcggccagcg
     4981 cgaccgcatc ggccagcgag gtgaccgtgc aggtcgggca gacctcaagt tcagcggccc
     5041 cggcgggcgg ggtcggtatg gctggtccag ccaaaacaac cgctataccg actgccgcaa
     5101 gcccctgccc caatcgacga atgaatggct gattattccg actgcgcatg gcgggaccac
     5161 cgttcccaga tcgcgacgac cagggtgatc accgccgctg ccgctgccag gtagaagccg
     5221 gtcgtgaagt tcgcttcggt gtggaactgg ccgattgtgc ccttgcccag gatcttcggc
     5281 gtgaactcct tgatcgagct gctcatcggc gctttcggat caagggtgtg accggcgtag
     5341 tacagccagc tgaagagatc aaccgcgaag atcacgggat agaggatcac cggcagccgg
     5401 agcagcgtgc gccagatacc ggtcaccagg aacgaggccg tcgccagcag cgccactgcc
     5461 gggatcgcat agaccgagat cgcctgctcg atcgtggcgg cgtcagtcag cttgatcatg
     5521 ccgatgtagt ggttgaggcc gtcgacctcg aagacattcc gcgccggctc catcttgttg
     5581 acgaaggtct ggatgaagag cccgcgtgga tactgcggcg cgtgcagggt gatctcccag
     5641 tagggataga acatcgaggc gaccagtaga atgccggcgc cggcttgcag aaagaaacgg
     5701 gccagccgcg catcgagtcc gatgcggacc tggctgagac catgagccgc cggcttgacg
     5761 gcctgggcag cctgattgtg atgcgttgcg accattgtgt gctgcctttc caatcagcgc
     5821 gccgggatcc agtcggatcg cggcgcacga accgcgacga tatgtctacg gcttgacgag
     5881 gaagtacccc atcatctcga ggtggagcgc cgagcagaac tcggtgcagt agtacgggta
     5941 gacacccgcg gtgtcggccg tgaatgtgat cgtggtcgac tcgcccggct cgatgctcag
     6001 gttgatgttc tgacccggca gcgcgaagcc gtgcgtcgcg tcctccgcca tctcgatgtt
     6061 ggtgatattc caggtcacct ccgacccgac cggcacttcg atgttgtccg gggtgaagtg
     6121 gctgcgcacc gaggtcatgt tgatcgtgac cttgttgcct tcgacgatca cgccctcctt
     6181 gcccggctcc ggcgcgccct ccttcttttt catttcgagc gaatcgaaac ccatctccgg
     6241 gtaggtgacc cacggctgga gcttcgaggc ccggatcgtc tgcgcgtagt gcggttcccc
     6301 aatcccgatc ggcatgtcgt agaggacctg catggtctcg ccggtgatgt cgatcaactg
     6361 gaagttctgt gggtgaagcg ggccaaccgg cgcgaaccgg tcgattgccc acttgttgag
     6421 cgcgatcacg aagttgcctt gcggcgtcac cgtgtcgccc tcgatcgccg tcagatggcc
     6481 gacgttgtag ttgaccggga tcttctcgat cagcgtccag ccgccctcga ccccctcgcc
     6541 gccgagcttc cacttggcga tcgcgctgtc gaggaagagg ctcgtgtagg cgttaccggc
     6601 gttgtcgaac tgcgtgtgca gcggacctaa gcccagctcc acctgcgcct cgacgcaggt
     6661 cgcgaagtcg agcaccggca cgccgaactc atcggtcgag tagccgccgg ccgcgatcgc
     6721 gtcctggatc ttctggaagc tgtagatggt cacgtgcggg tcgagcttgc cggccacgac
     6781 caggtacttg ccgtccggag tgacatcgac gccgtgcggg ctctttggct caggcacctg
     6841 gtagatgagc ccattcgaga cggcatccgc catccggatc atcttcatgc ccttgacgtc
     6901 ttcgccaccg ccgttggcga tcacttccgc ggccttgcgc cagttgatga ccgacagata
     6961 atccatgtcg cgcatcgagg cgccggcctc aaccgggggc tgaccgtcca tgatgccgcc
     7021 gagcgccatc tcggtattga gcgagttgga gaacgaccag ccatcgctcg ggcccttgcc
     7081 ggcatctgcg aggtcctgcc agtacggcgg gatctcgatc gagaacgact gcgaggggtc
     7141 gatccggccg gtttcgcggt tgaacttcca gtaggtcatt gctcctcggt acgaggtctt
     7201 gtaatcggag aggggcgcgt actcccaacc aaatggcgcc gcgtactgcg agccgtcgat
     7261 gatgtagtcg gtattgggcg taacgaaggc gccgccgtgg ttgctgatga tgtgcggatt
     7321 cttgacgatc tgcttcgtct cgaagtcgcg caggtcgatc acggccatgc gggcatgggc
     7381 cttgtcgtta atgaagagga attgcccgtc gtactcgcca ttggtctcgc tcagaccggg
     7441 gtgatgcgtg tccgcccagg tgagcgtctt gccattcatg ccgccggctt cgagcatcgc
     7501 ccgggagccg tcatcgaagc cgaatccctg ccatggctcc ggcgtgaaga cgccgatcac
     7561 cttgagcaat cgcatcgacg ggacgccgac aacgagcacc tgcccgccat ggccgcccga
     7621 cgcgaagatg tagtactcgt cccatttgcc gctgggtacg taagtcttga gcgcggccgt
     7681 catgtcctgg ggggtgagcc cacgcgcatt ggcgatatcc tcagcggatt gcgcctgcgc
//
LOCUS       DATAOZ010000210         4414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8246285, whole genome shotgun sequence.
ACCESSION   DATAOZ010000210 DATAOZ010000000
VERSION     DATAOZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8246285"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VHR64_05590"
     CDS             complement(<1..792)
                     /locus_tag="VHR64_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX3302335.1"
                     /translation="MLKLPIAPARTTMATALRQALAAPRIQRGRQLSARVRYGLVVVA
                     VGVATVLMTAMRDHLGALSIILLYLLLCFILTLFVGSGPAALAAVLAVLAFNYFFVPP
                     IHTFDVAQTDNLVALFVFLGVAVITGRLVANTRARAEVALREQDRTALLYELNAALIG
                     DVTLDAILATIVASVVQIYGARSGRILLPNPEGRLEERARFPESAMPHIDRQSLAMAS
                     LVMERGRSAGRSRSERRVIAPAALRPSDADVLYLPIATTERRIGVL"
     gene            1331..3469
                     /locus_tag="VHR64_05595"
     CDS             1331..3469
                     /locus_tag="VHR64_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX3302336.1"
                     /translation="MKEPSPDGDRSTDNESPLPDERLASGMYPRKVRTRRSVGPKIQG
                     DLSGKQQIQGERAADRYIRVVRAQHGNFVPAGPGLLVATPGASEPAGAISRGYSKVKR
                     LLIGAPLSTAEAAHERLTKVKALAVLSSDALSSVAYATEEILRVLLVAVGVSVLNVSL
                     PIGVAIMVLIVVVGMSYRQTIKAYPHGGGSYIVAKDNLGERPALTAGAALMADYTLTV
                     AVSIAAAVAALVSAFPSLHTHRVGMGVAFILLVTVLNLRGIRESGSIFAVPTYLFLIG
                     MFVMIAVGFARTAANGFEAEAVPADAHDLAGTGALTIFVVLRAFSSGCSAMTGVEAIS
                     DGVPAFKPPEWINARKTLTAMVTILVLTFGSITFLAHQFGTFPMEADQPNYETVISQI
                     ARHAFGGANAAYFYIQFATMAILVLAANTAFSDFPRLAYFLARDRYMPRQFTYRGDRL
                     AFTTGIVTLGLLAGIVLVIFGGEVEHLIPLYALGVFTSFTISQFAMFTRWQQRREPGW
                     KMGRVINLIGATATGIVAVVVALTKFLAGAWMTALVILLLILMMRSIHRHYQRASTEL
                     ATGTPIDPREIRHTVIVPVSSMNRVALQTLAYAQSISDDVTAVHVMDDEGDIETFRTK
                     WEELGLDIPLVLIESPYRALVSPLLKYIDEIDRQRQDDTLTVVLPEFVARHWWEHLLH
                     NQTALRLKAALLFRPNTVVTSVPYHLERRA"
     gene            complement(3536..4015)
                     /locus_tag="VHR64_05600"
     CDS             complement(3536..4015)
                     /locus_tag="VHR64_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3302337.1"
                     /translation="MNATDVLRYGQLTFLSGLDRLPESEWESPGVCGYWSVKQIIAHI
                     ASYEAVLADILKSFTGSGPTPALDRFLGNNVAFNDDEVAARANLSPSETLEELRAEHE
                     RVMGLLDAIPVETLRSTGTMPWYGDEYSIEDLIVYQYYGHKREHVAQIWVFRDHLAG"
     gene            complement(4036..4398)
                     /locus_tag="VHR64_05605"
     CDS             complement(4036..4398)
                     /locus_tag="VHR64_05605"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3302338.1"
                     /translation="MSTTDVATDTRTVIIRFNEAFDRHDVAAISALMTEDVVFENTSP
                     FPDGERFVGNAAVSAYWQKFFDESPHAHFDAEEMVVMDDRAVVRWRYSWGTGHVRGID
                     LFRVRDGKVAEKFAYVKG"
BASE COUNT          769 a         1447 c         1375 g          823 t
ORIGIN      
        1 gagcacgccg atcctccgct cggtagtggc gatcggcagg tacagcacgt cggcgtcgga
       61 cggccgcaat gctgccggag caatcacgcg ccgctcgctc cggcttcgcc cggccgaccg
      121 cccccgttcc atcacgagcg aggccatggc gaggctttgg cggtcgatgt gcggcatcgc
      181 cgattccggg aaacgggccc gctcctcgag gcgaccctca gggttcggca acaggatacg
      241 gccgctcctg gcgccgtaga tctggaccac gctcgcgacg atcgtggcca ggatagcatc
      301 gagcgtcacg tcgccgatca gggcggcgtt gagctcgtag aggagtgcgg tccgatcctg
      361 ttcccgcaag gcaacctcgg cccgggcgcg ggtattggcg acgagccgcc cggtaatcac
      421 ggccacaccg agaaaaacga agagcgccac cagattgtcg gtctgggcga cgtcgaaggt
      481 gtggatcggc ggcacgaaga agtagttgaa tgcgagtacc gcgagcactg ccgccagggc
      541 cgccggaccc gagccgacga agagggtcaa gatgaagcag agcaggaggt agagcaggat
      601 gatgctcagc gcgcccaggt gatcccgcat cgcggtcatc agaacggtgg ccactcctac
      661 ggcgaccacc acaagcccat accgaacacg tgccgacaat tgccgaccgc gctggatacg
      721 cggcgctgcc agcgcctggc gcaacgcggt ggccatcgtc gttctggccg gtgcgatggg
      781 caatttaagc atcaggttgc gttgttccct ccacctgaca cgtggtcgtc cctatcaaat
      841 cctacgccca tctcagacgc ggcgagcacg gccggttttc taacaacttt ctaatgcctg
      901 gagccggttt tcttatgcgg tgcaaatgcg aatgccagta gttttttctg ctggcagtga
      961 tggtcgccgc tcgggccgcc gtcaccgcat tcggaactgg cgctccaatg tgcgaaccgg
     1021 acatctccct ccaatcgctg atcgagccag cctgtcgggt gatcgctgac tgaaagatcc
     1081 cgtcgatcac ggtgtcgata gggaggccac ttgcgccgga ggagttgatc gcgttccgag
     1141 cgtatgcagg caactgtggc gtgctactca gcgagattga accggggact atagtggtgc
     1201 gtggagggat cagcgtggtt gcgcggtccc gtcccggctg gatccgctcg cttttgggcc
     1261 ggtttggtcc gcgagcggtc gcggtggatt cccccatgaa tccaggctgg gcaccatgac
     1321 gtcagatggc atgaaagagc cgtccccgga cggcgaccgt tcaaccgaca acgaatcccc
     1381 gctccccgac gagcgacttg catccgggat gtatccccgc aaagtgcgga cgcgccgatc
     1441 ggtcggtccg aagatccagg gcgacctgag cggaaagcag cagatccagg gagagcgcgc
     1501 ggccgaccga tatatccggg tggtgcgcgc ccagcatggc aacttcgtgc cggctggtcc
     1561 gggcttgctg gtggctacgc cgggcgcctc ggagccagcg ggagcaatca gccgcggcta
     1621 ctcgaaggtc aagcggctcc tgatcggtgc accgctctcg accgcggaag ccgcgcatga
     1681 gcggctaacc aaggtcaagg ctctggctgt cctctcatct gacgccctct cctccgtggc
     1741 gtatgcgact gaggagatcc tgcgcgtgct gctggtcgcg gtcggcgtgt cggtcctgaa
     1801 cgtcagcctg ccgatcggcg tggcgatcat ggtgttgatc gtcgtggttg gcatgtccta
     1861 ccggcagacc atcaaggcct acccgcatgg cggcggcagc tacatcgtgg ccaaggacaa
     1921 cctcggtgaa cgtcccgccc tgaccgcggg cgccgctctc atggccgact acaccctgac
     1981 tgtcgcggtc tcgatcgccg ccgccgtggc cgcgctcgtc tcggccttcc cctcgctgca
     2041 cacgcaccgg gttggcatgg gcgtcgcgtt catcctgctg gtgacagttc tcaacctgcg
     2101 cggcatccgg gagtccggct cgatctttgc cgtgccgacg tacctgttcc tgatcggcat
     2161 gttcgtcatg atcgcggtcg ggtttgcgcg cacggcggcg aacggattcg aggcggaagc
     2221 ggttccagcc gatgcgcacg acctcgccgg caccggggcg ctcacgatct tcgtcgttct
     2281 ccgtgcattc agttccggct gctcggcgat gaccggtgtc gaggcgatct cggacggcgt
     2341 gccggccttc aagccgccgg aatggatcaa tgcccgcaag acgctgacgg cgatggtcac
     2401 gatcctcgtt ttgaccttcg gcagcatcac cttcctggcg caccagttcg ggacgttccc
     2461 gatggaagcc gatcagccca attacgagac ggtcatctcg caaatcgcgc gacacgcctt
     2521 cggcggcgcc aacgcggcct acttctacat ccagttcgcg acgatggcaa ttctggtcct
     2581 ggcggcaaac acggccttct ccgacttccc ccgcctggcc tacttcctgg cgcgggaccg
     2641 ttacatgccg cggcaattca cctatcgcgg cgatcgcctc gccttcacga ccggcatcgt
     2701 caccctcggc ctgctggccg gaatcgtgct ggtgatcttc ggcggcgagg tcgagcacct
     2761 gatcccgctc tacgcgctgg gcgtcttcac ctcgttcacg atcagccagt tcgccatgtt
     2821 cacgcgctgg cagcagcggc gcgagccagg ctggaagatg ggccgggtca tcaacctgat
     2881 cggcgcgaca gcgacgggca tcgtggcggt cgtggtggca ttgaccaagt ttttggccgg
     2941 cgcctggatg accgcgctcg tcatcctgct cctgatcctg atgatgcgct cgatccaccg
     3001 gcactatcag cgggcctcga ccgagctggc gaccggtacc ccgatcgatc cgcgcgagat
     3061 ccgccacacc gtaatcgtgc cggtctcgtc gatgaaccgc gtcgccttgc agacgctcgc
     3121 ctatgcgcag tcgatctcgg acgacgtgac cgcggtccat gtcatggacg acgagggcga
     3181 catcgagacg ttccgaacca aatgggagga gctggggctc gacatcccgc tggtcctgat
     3241 cgagtcaccg taccgggcgc tggtctcacc gctgctcaag tacatcgacg agatcgatcg
     3301 ccagcggcag gacgacacgt tgacggtcgt gttgccggag ttcgtggcgc ggcactggtg
     3361 ggagcacctg ctccacaatc agacggccct gcggctcaaa gccgcgctgc tcttccgccc
     3421 caacaccgtc gtaaccagcg tcccgtacca cctcgaacga agagcgtgat ccgcccagga
     3481 ggttttgggt tttaggtttt cgtttttctt tccctcaacc ctccacgctc aactctcaac
     3541 ccgccaaatg atcgcggaag acccatatct gggcgacgtg ctcccgcttg tggccgtagt
     3601 actggtagac gataagatcc tcgatggagt attcgtcgcc gtaccacggc atcgtccccg
     3661 tcgagcggag cgtctcgacc gggatcgcgt cgagcagccc catgacccgc tcatgctcgg
     3721 ctctcagttc ctcaagcgtc tccgatggtg agagattagc cctggccgcg acctcgtcat
     3781 cattgaaggc cacattgttg ccgaggaagc ggtcgagcgc cggagtcggg ccactaccgg
     3841 taaagctctt caggatgtcg gccagcactg cctcgtacga ggcgatgtgg gcgatgatct
     3901 gcttcaccga ccagtagccg cagacgccgg gcgattccca ctcgctctca ggcaggcgat
     3961 ccagaccgga caaaaacgtc agttggccat acctgagcac atcggtcgcg ttcatggtgc
     4021 ttctcccgtt tcggctcagc ccttcacgta ggcgaacttc tcggccacct tgccatcacg
     4081 cacgcggaag agatcgatcc cgcggacgtg gcctgtcccc cacgagtagc gccagcgaac
     4141 gacggcgcga tcgtccatca cgaccatctc ctcggcgtcg aagtgggcgt gcggcgattc
     4201 atcgaagaat ttctgccaat aggcgctcac cgcggcattg cccacgaagc gctcgccgtc
     4261 gggaaaggga gacgtgttct cgaagacgac atcctcggtc atcaacgccg aaatcgcggc
     4321 gacgtcgtgg cgatcaaacg cctcgttgaa gcgaatgatg acggtccggg tatccgtagc
     4381 gacatctgtt gtgctcacgt gctcctcctc gcgc
//
LOCUS       DATAOZ010000211         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8508464, whole genome shotgun sequence.
ACCESSION   DATAOZ010000211 DATAOZ010000000
VERSION     DATAOZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8508464"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1786)
                     /gene="mutL"
                     /locus_tag="VHR64_05610"
     CDS             complement(71..1786)
                     /gene="mutL"
                     /locus_tag="VHR64_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX3302339.1"
                     /translation="MPIRVLTEQTIGKIAAGEVIERPASIVKELLENSLDSDASQISI
                     AFADGGLREIRIADNGCGISSEELPLAIQRHATSKLREFEELSRVATLGFRGEALASI
                     AAVARLQIRSAEAGRPSSLLSTEFGKTPDIRAAAGSAGTTVTVSDLFENVPARRKFLR
                     QPSTESTAIQRVVAAYAASRPDVAFQLEIDGRPSFATDGSGELQSAATAVFGQEVGHA
                     FLELPGLDESARVPGVSASGWVTGPAVTRSHRQQMHVFVNGRLIQHRTLNFVIEECFH
                     TLLMVGRHPVVMIRIEIDPELVDVNVHPTKAEVRFQDERAVCRAVQRAVHAALAGAPT
                     DELPRVSFAIPSIPPMPAMQPTLMPTPARAPEPGDEEAMTNPPVSPRSVPMLRVLGQV
                     SASFIIAEGPEGLYLIDQHAAHERVMYEKIRSQISSRTTDQQPLLDPMVIELDPHESA
                     VMERSLEELNQIGFEIEPFGEQAFTLRAIPAIMTGVDIRERIHLILRELAEGGVGESW
                     LDSVAISAACHTSIRAGQVLSISEMRELVNQLEQTEHPRACGHGRPTMLLMSQGDLQK
                     QFSRR"
     gene            complement(1812..2408)
                     /locus_tag="VHR64_05615"
     CDS             complement(1812..2408)
                     /locus_tag="VHR64_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX3302340.1"
                     /translation="MTSRLDRAYFARPTVEVARDLVGCILVRKLDGERLSGRIVETEA
                     YGNESDLASHSAVYQRTRIEIMRAEPGTVYVYRSYGIHFCFNIVAHEAGEAGAVLIRA
                     AEPIAGRSIMAERRGVATDGPIANGPGRLGQAFSLERADTGRDVVADPDIYLMPRQGA
                     LLVAESTRIGITRDTHRLWRFYDQESPALSRPHRTART"
     gene            complement(2401..>2591)
                     /locus_tag="VHR64_05620"
     CDS             complement(2401..>2591)
                     /locus_tag="VHR64_05620"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302341.1"
                     /translation="RIEKELANARGEIERLTRQLSNPNFIERAPEKLVTDQRIRLALV
                     EEQVGVLTRRLADLSDVD"
BASE COUNT          453 a          823 c          831 g          484 t
ORIGIN      
        1 gctagatagt cttttcctga gattggctgg gggagacgga tcggcgcagt gccggtccgt
       61 ttctcgctgc ttagcggcgc gagaactgct tctggaggtc gccctggctc atcaggagca
      121 ttgtcgggcg accgtgtcca cacgcgcgtg ggtgctcagt ctgttcgagc tggttgacta
      181 gctcccgcat ctcggaaata gagaggacct gcccggcccg aattgacgta tggcaggcgg
      241 cgctaatcgc gaccgaatcg agccaggatt ctccgactcc gccttcggcc aactcgcgca
      301 ggatgaggtg gattcgttcc cgtatgtcga caccggtcat gatcgccggg atggctcgaa
      361 gagtgaatgc ctgctcgccg aacggctcga tctcgaagcc gatctggttg agctcctcga
      421 gtgaacgctc catgaccgcc gattcgtgcg gatcaagttc gatgaccatc ggatcgagca
      481 acggttgctg atcggtcgtg cgcgagctga tttgcgagcg gatcttctcg tacatcactc
      541 gctcatgcgc ggcatgctgg tcgatcagat acagcccctc cggcccctcg gcaatgatga
      601 acgaagcact gacctgcccg aggacgcgca gcattggaac cgaccgcggt gaaacgggcg
      661 ggttggtcat cgcctcctcg tcgcccggtt ccggagcgcg tgccggtgta ggcatcaggg
      721 tcggctgcat cgccggcatc ggtggaatgg acggaatcgc gaacgagacc cgcggcagct
      781 catcggtcgg cgcgccggcg agcgcggcgt ggacggctcg ctggactgcc cggcaaaccg
      841 cccgctcgtc ctggaatcgg acctcggcct tggtcggatg cacgttgaca tcgaccagct
      901 cggggtcgat ctcaatccgg atcattacga ccgggtgccg ccccaccatc aggagcgtgt
      961 gaaagcactc ctcgatcacg aagttgagcg tccggtgctg aatcagccgg ccgttgacga
     1021 agacatgcat ttgttgccgg tgcgatcgcg tgaccgcggg acctgtcacc cacccggatg
     1081 ccgaaacacc cgggactcgc gccgactcat caaggcccgg caactccagg aaggcatgtc
     1141 cgacctcctg gccgaacacc gccgtcgccg ctgattgcag ctctcccgag ccatcagtgg
     1201 caaatgatgg tcgcccatcg atctcgagtt ggaacgcgac atctggccgg ctagcagcat
     1261 aggcagccac cacgcgctgg atggcggtcg actcggtcga tggctggcgc aggaacttgc
     1321 gccgggctgg tacgttctcg aagaggtcgc tgactgtcac cgtcgtcccg gcggacccgg
     1381 ctgcggcgcg gatgtcaggt gttttcccga attcggtact gagcaatgac gacggccgcc
     1441 cagcttcagc tgaccggatc tgaagcctcg cgacggctgc gattgaagcc agagcttcgc
     1501 cacggaagcc gagcgtcgcg acccggctca attcctcgaa ttcacggagc ttggaggtcg
     1561 cgtgtcgctg gatcgcgagc ggcagctctt cggaggaaat cccgcagccg ttgtcggcga
     1621 tgcggatctc gcgcaggccc ccgtcggcga aggcgatgct gatctggctg gcatcgctgt
     1681 cgagcgagtt ctcgagcagc tccttgacga tcgacgccgg tcgctcgatg acctccccgg
     1741 cggcaatctt cccgatggtt tgctctgtca ggacgcgaat cggcatggtc aagatgctag
     1801 cacgggcgca gtcacgtcct cgccgtccga tgaggtcggg aaagtgccgg cgattcctgg
     1861 tcgtagaagc gccacagtcg atgcgtgtcc cgcgtgatgc cgatccttgt tgactccgca
     1921 accagtagcg cgccctgacg aggcatcaag tagatgtcgg ggtccgcgac gacgtctcgt
     1981 ccggtatcgg ccctttcgag cgagaacgcc tgcccaagcc gtcctggtcc gttcgcaatc
     2041 ggtccgtcgg tagccacgcc acgccgctcg gccatgatcg aacgaccagc gatcggctcg
     2101 gcggcccgga tcaggaccgc acccgcctcg cccgcctcgt gcgcgacgat gttgaagcag
     2161 aaatggatgc cgtagctgcg gtagacatag acggttcccg gctcggcccg catgatctcg
     2221 attcgcgtgc gttgatagac ggcggagtgc gaagcaagat cggactcgtt gccgtatgcc
     2281 tcagtctcga cgatccggcc ggagagccgt tcgccgtcta acttccgcac gaggatacag
     2341 ccgaccagat cgcgtgcaac ctcgactgtc ggccgggcaa agtacgcgcg atcgagccgg
     2401 ctagtcaacg tcgctaaggt ctgccaaccg gcgggtcagg actccgacct gctcctcgac
     2461 cagggccaac cggatgcgct gatcagtcac caatttctcc ggcgcgcgct cgatgaagtt
     2521 cgggttggag agctgccggg tcagccgctc aatctcgccg cgggcgttgg ccagctcttt
     2581 ttcgatccgg a
//
LOCUS       DATAOZ010000212         7250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5087682, whole genome shotgun sequence.
ACCESSION   DATAOZ010000212 DATAOZ010000000
VERSION     DATAOZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5087682"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..2141)
                     /locus_tag="VHR64_05625"
     CDS             complement(303..2141)
                     /locus_tag="VHR64_05625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX3302342.1"
                     /translation="MATTTRRRTGTRYSFEQFTAIRRYQPTLTFSPDGREIAYVVNTS
                     GQFNIWRQSSAGGFPHQLTLFTDQTPRDISWSPDGQTIAFAADRDGDEYKQIYTIPAR
                     GGRISAQTEAPRVRHELAPEAWSPDGTSLLIAGNDRSETDQDVMIRDLATGETRRVVD
                     YGGMYFPVTWAPDGKSVTVVDFKSNTNMDLYVLTVDNEEMQHLTPHEGEIQYLPGPWA
                     ADGSGFYVISDEGREFAGLAFWDLKEGMRWVDAPDADVENVALSGDGRYLAWIVNQEG
                     YSALKVRDLRSNRDVALPEMPKGVIDALTMSHSGGMVGFLMAEATHPHEVYVLDLERS
                     TLTRLTESMLGGIDEADLSAPELIHYPTHDGRKIPAWLYRPEGDGPFPVILSIHGGPE
                     WQERPTYNYSGLYQYLLTRGIGILAPNVRGSTGYGKSYQKLIHHDWGGAELGDFKAAA
                     DYLKGLDWVDGDRLGVYGGSFGGFATLSCLTRLPDYWAAAVDIVGPSNLVTFAKAVPP
                     TWRRFMDQWVGNPETEVDFLMERSPITYVDQVTAPLFVIQGANDPRVVQNESDQIVER
                     LRARGVEVKYDVYEDEGHGFTRRSNELKALGDTAGFFETHLLGAEA"
     gene            complement(2704..5679)
                     /locus_tag="VHR64_05630"
     CDS             complement(2704..5679)
                     /locus_tag="VHR64_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX3302343.1"
                     /translation="MNELFGIPMSGILTALVALLAVCLLSVAWVAWRRPVIFKLGVRN
                     IPRRKAQTTLIVVGLMLSTLIISAALGSGDTLHYSTTSEVYNSLGNVDEVVVYSPDGK
                     GDASTSQSNNIDASALATVKTALAGNENVDGVMPVLWKSMPVMNMTSGLAEPEVGVVG
                     VDPSQLGPFGGIKATNGDTIDIAALGANEIVVNAKTADKVDAKVGDTIVVYYNNQPVQ
                     LTVAAIAKDTVLSGSLNTGMPGMVTSLARLQEITGLANTYSLIAISNKGGVRDGKAHT
                     DAVVTQLETALAGTKLGIDPIKKDTVDEVESFSALFTSLFLVLGLFSIAAGILLIVLI
                     FTMLAAERRSEMGMARAVGTHRRQLIQQFVAEGSGYALLAGLVGSALGVAAAYGIGYA
                     IQALVGDELSITPHVEPRSLMVAYCLGVVITFLAVVGSSWKISRLNVVAAVRDIPDVQ
                     PSRRSWKVLLWGTLMLLGGGLLTMSGQSADKAFPFYAGMSLLPFGVALFARFFGAPPR
                     LVFSAVGAWLLFLWLLPEGPSNALWGELDGGFEMFFLSGIFMVVGATVLLVQNLDVLL
                     KGVSLLGGLFKSKTPAVRTAIAYPGASRGRTGMAIAMFSLIVFSLVMMSTMNENYTAL
                     FLGDEAAAGWDVRADTAMSNPVSDVKGTLEQNGIDTSGFTAIGTVTTPNPFASRLRMS
                     GTSDWKPYEVRGMNQSFIEDSSLTFGQRAEGYDSDKAIIQALLTQPNVAVVDVFALGG
                     NGDFGGNGDGFQLSGVSVDDKTFAPAPVEVINPETGQPVTVTVIGVLDSKIGTLTGLY
                     ANQATIDTIYPSAVMTSYYIALADDSQAETVANKIESTLLPYGVQAASIQEELADGQK
                     QSASFLRIIEGFMGLGLIVGIAAVGVIAFRSVVERRQQIGVLRALGFQKTMVSLSFLI
                     ETAFIVGMGVISGTALGLVLARNLFMSDDAAKSASFLVPWPTITVILSATMAVALLMA
                     WIPSRQAARIAPAEALRYE"
     gene            complement(5736..6524)
                     /locus_tag="VHR64_05635"
     CDS             complement(5736..6524)
                     /locus_tag="VHR64_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302344.1"
                     /translation="MLSLLNRKGSTATTSPIITGPDPNLIIEARDVVKTYNTGTQKVA
                     ALKGVSFGATSGEMVAVMGPSGSGKTTLLNTLSGLDTIDSGAIWLEGEDLSTMKDRKR
                     TSYRAKRMGFIFQVYNLLPVLSAVENVELPLLVSGVSPKDARQKALAALETVELSNRA
                     NHRPAELSGGQRQRVTIARSLVNDPAIVWADEPTGALDSKTATDIMALMGKLNEENGL
                     TFVLVTHDRGIGAECDRVVYMVDGEIVDQSINRPLAYAEDTAAD"
     gene            complement(6716..6991)
                     /locus_tag="VHR64_05640"
     CDS             complement(6716..6991)
                     /locus_tag="VHR64_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302345.1"
                     /translation="MSGAMGDSRVIYVGSSRQRTGRWFGMFPRYEPEHEWRQRLATEM
                     DAACQEMSRRGLSLVQVVPVLSSVSMQGAWTEGAWLYFTSARPASAL"
BASE COUNT         1337 a         2556 c         2059 g         1298 t
ORIGIN      
        1 ccaaggcaga acgccaccac gagctgaccc gcagcgattc agtcgccgcc aagaaaccgg
       61 tccagcgccc aaccgttctg gcgcagcgtg tctcactgat cagccgcctg gtccaccccc
      121 gcctccgccc cgcctaacca atgccaacca gacccaacca atcgccaaga cgatcgcccg
      181 gatgaaaacc acccgggcga tctgctgtcg acaagtcaat cagcgccaga ccatttcagg
      241 ctcctcgact cctcgacttc tcgactcact tcgcgcctcc gcgccttcac gcctccgcgc
      301 cttcacgcct ccgcgcccaa gagatgcgtc tcaaaaaacc ccgccgtatc ccccaacgcc
      361 ttcaactcgt tcgaccgccg cgtgaagccg tggccctcgt cctcgtagac gtcgtacttg
      421 acctcgaccc cgcgcgcgcg cagccgctcg acgatctggt cactctcgtt ctggaccacc
      481 cgcggatcgt tcgcgccctg gatcacgaac aacggcgccg tcacctgatc gacataggtg
      541 atcggcgagc gctccatcag gaagtcgact tcagtctccg gattcccgac ccattgatcc
      601 atgaaccggc gccaggtcgg cggcacggcc ttggcaaagg tcaccaggtt cgacggcccg
      661 acgatgtcca cagccgccgc ccagtaatcg ggtagccggg tcaagcacga gagcgtcgcg
      721 aagccgccga acgacccgcc atagacgccg agccggtcac catcgaccca atccagcccc
      781 ttcaggtagt cggccgccgc cttgaagtcg cccagttccg ctccgcccca gtcgtggtgg
      841 atgagcttct ggtagctctt gccgtagccg gtcgagccgc gcacgttcgg cgccaggatg
      901 ccgatccccc gcgtcaacag atattggtag agaccgctgt agttgtaggt cggccgctcc
      961 tgccactccg gaccaccgtg gatcgagagg atgaccggga acggtccgtc tccctcgggc
     1021 cggtacagcc aggccgggat tttccgccca tcgtgcgtcg ggtagtggat cagctccggc
     1081 gcgctcagat cggcctcatc gatcccgccc agcatgctct ccgtcaaccg cgtcagcgtg
     1141 ctccgctcga gatcgagcac atagacctcg tgcggatgcg tcgcctcggc catcaggaat
     1201 ccgaccatcc cgcccgagtg cgacatcgtc agggcgtcaa tcacaccctt gggcatctct
     1261 ggcagcgcca cgtcccggtt cgagcgcaga tcccgcacct tgagcgcgga gtacccttcc
     1321 tggttgacga tccacgccag gtagcgacca tcgcccgaga gcgcgacatt ctcgacgtcg
     1381 gcatccggcg catcgaccca gcgcatgccc tccttcaggt cccagaaggc gagcccggcg
     1441 aactccctcc cctcatcgct gatcacgtag aagccggagc catcggctgc ccacggaccc
     1501 ggcaggtact ggatctcgcc ctcatgcggg gtcaggtgct gcatctcctc gttgtcgacg
     1561 gtcaggacgt agagatccat gttcgtgttc gacttgaagt cgaccaccgt caccgatttc
     1621 ccatccgggg cccacgtcac cgggaagtac atcccgccgt agtcaaccac ccggcgtgtc
     1681 tcgccggtcg cgaggtcacg gatcatcaca tcctggtcgg tttcgctgcg atcgttgccg
     1741 gcgatcagga gtgatgtccc gtccggcgac cacgcctccg gggcgagctc gtggcgcaca
     1801 cgtggtgcct ccgtctgtgc cgaaattcgc ccgccccggg cggggatcgt atagatctgc
     1861 ttgtactcat cgccatcgcg atcggccgca aaggcgattg tctgcccgtc cggcgaccac
     1921 gagatatcgc ggggagtctg atcagtgaag agcgtcaact gatgcggaaa cccgcccgcg
     1981 ctgctttgcc gccagatatt gaactgcccg gacgtattga ccacgtaggc aatctcccgc
     2041 ccatccggcg aaaaggtcag cgtcggctgg taccgccgga tcgccgtgaa ctgctcgaac
     2101 gaataccgcg tcccggttcg acgccgcgtt gtcgttgcca ttgcaacctc ctcgactaac
     2161 cgcttcaacg aaagccacgt cccagcatac ccaagccaaa tcgggaaaca tcactcctaa
     2221 gaggtattcg caacacccac ggccaagcga ccggtattgc acgcctcgct gtcccatccc
     2281 gagaaccggg tcctggatcg ggaacgagtc tgccgtaccg cccggggtaa atgcccgggc
     2341 tgaacacagg gaagcccact aaacgggctg aagaagagat ccgcaatcca ggagatatcc
     2401 ggcttcgccc cgaactgaag gccggggctg aatcaggata aggcgactga agccgactcc
     2461 tccttcagtc cctttagtgg acttatcgtc acttagcccg gacctttagc ttcgggcggc
     2521 gccgaacagc tggttggata ttcggagcct gtcatcgggg aacgatcgtc accacctggg
     2581 cgatcgccgg ggagcatacg tcgcaggcaa agtggccggg caagatcagg ggtcaggcag
     2641 caaagccacc tgacccccga tccctgacca ctgacgcccg aagtccgacg tctgccgtct
     2701 cgtctactcg taccgcagcg cctccgccgg ggcgatccgc gccgcctggc gggatggaat
     2761 ccacgccatc agcaacgcca ccgccatcgt cgcgctcagg atcacggtaa tcgtcggcca
     2821 cggcaccagg aacgaagcgc tcttcgccgc gtcatcgctc atgaacaggt tccgcgccaa
     2881 caccagcccg agcgccgtcc ccgagatcac gcccatccca acgatgaatg cagtctcgat
     2941 caggaagctc aacgagacca tcgtcttctg gaacccgagc gcccggagga cgccgatctg
     3001 ctgccgccgc tcgaccaccg accggaaggc aatcacgccc accgccgcga tcccgacaat
     3061 cagtccgagt cccatgaagc cctcgatgat ccgcaggaag ctggccgatt gcttctgacc
     3121 gtccgccagc tcctcctgga tcgaggccgc ctgcacaccg tacggcaaca gcgtcgattc
     3181 aatcttgttc gccactgtct ccgcctggct gtcatcggcc agtgcgatgt agtacgaggt
     3241 catgaccgcc gacgggtaga ttgtgtcgat cgtcgcctgg ttagcgtaca ggccggtcag
     3301 cgtcccaatc ttgctgtcga gcacgccgat caccgtcacc gtcaccggct gccccgtctc
     3361 cgggttgatc acctcgaccg gcgccggcgc gaacgtcttg tcgtccaccg agactccgct
     3421 cagctggaag ccgtcgccat tcccgccgaa gtcgccgttg ccgcccagtg cgaagacatc
     3481 gaccaccgcc acgttcggct gcgtcagcag cgcctggatg atcgccttgt cgctgtcgta
     3541 tccctcagcg cgctgcccga aggtcaggct gctgtcctcg atgaaggact ggttcatgcc
     3601 ccgcacctcg tatggcttcc agtcgctcgt gccggacatc cggagccgcg acgcgaacgg
     3661 attcggcgtg gtcaccgtgc cgatggccgt gaaaccgctg gtgtcgatcc cgttctgctc
     3721 gagcgtgccc ttgacatcgc taaccgggtt ggacatggca gtgtcggccc ggacatccca
     3781 gccggcagcc gcctcatcgc cgaggaacag cgccgtgtag ttctcattca tcgtcgacat
     3841 catgaccagc gagaagacga tcaggctgaa catcgcgatg gccatgccgg ttcggccccg
     3901 ggaggcaccc ggataggcga tcgctgtccg caccgccggc gtcttcgact tgaacagccc
     3961 acccaggagc gacacaccct tgagcagtac gtccaggttc tggaccagca gcactgtcgc
     4021 accgaccacc atgaagatgc cggacaggaa gaacatctcg aacccgccgt ccagttcacc
     4081 ccaaagcgcg ttgctcggcc cctccggcaa cagccagagg aagagcagcc aggcgccgac
     4141 cgcactgaag accagccggg gcggtgcccc gaagaagcgg gcgaacagtg ccacaccgaa
     4201 cggcagcagg ctcatgccgg cgtagaacgg gaacgccttg tccgcgctct ggccgctcat
     4261 ggtcagcagt ccgccgccca gcagcatcaa cgtgccccag agcagaacct tccagctccg
     4321 cctgcttggc tggacatccg ggatatcccg gaccgccgca accacgttca gccggctgat
     4381 cttccacgaa gatccaacga ccgccaggaa tgtgatcacg acgcccaggc agtacgccac
     4441 catcaagctg cgcggctcga cgtgcggcgt aatcgacagc tcgtcgccca ccagcgcctg
     4501 gatcgcatat ccgattccgt acgccgccgc gacgccgagc gccgagccga ccaggccggc
     4561 cagcaatgca tacccggagc cttcggccac aaactgctga atcagctgac gccggtgcgt
     4621 gccgaccgcg cgggccatgc ccatctcgga ccgccgctcg gccgccagca tcgtgaagat
     4681 caggacgatg agcaggatac cggccgcgat cgagaatagc ccgagcacca ggaagaggct
     4741 cgtgaacagg gccgagaagc tctcgacctc atcgaccgtg tccttcttga tcggatcgat
     4801 gccaagcttc gtgccggcca gcgccgtctc gagctgcgtc accaccgcat cggtgtgcgc
     4861 cttgccatcc cgcacgccgc ccttgttcga aatcgcaatc aggctatacg tgttcgcaag
     4921 accggtgatc tcctgcaacc gcgccagcga cgtcaccatg cccggcatgc cggtattcag
     4981 cgagccgctc agcaccgtat ccttcgcgat cgctgccacc gtcaattgca ccggttggtt
     5041 gttgtagtac acgacgatgg tatcgccgac ctttgcgtcg accttgtcgg ctgtctttgc
     5101 attgaccacg atctcgttcg cgccgagcgc ggcgatatcg atcgtgtcgc cgttcgtcgc
     5161 tttgataccg ccgaacgggc cgagttggct cgggtcgact ccgacgacgc ctacctcggg
     5221 ctccgccagg ccgctggtca tgttcatgac cggcatcgat ttccagagca ccggcatcac
     5281 gccgtcgacg ttctcattgc cggccagcgc ggtcttcacc gtggctaagg cgcttgcatc
     5341 gatgttgttg ctctgtgagg tcgaggcgtc gcccttgcca tctgggctgt acaccaccac
     5401 ctcatcgacg ttgccgagcg agttgtagac ctcggaggtg gttgagtaat ggagcgtgtc
     5461 gccgctgccc agtgccgccg agatgatcag cgtcgagagc atcaggccga ccacgatcag
     5521 tgtcgtctgc gccttgcgcc gcggaatgtt ccgcacgccg agcttgaaga tgaccggccg
     5581 gcgccaggcg acccacgcca ctgacagcag gcatacggct agcagcgcca ccagcgcggt
     5641 caggatcccc gacatcggga ttccgaatag ttcgttcaca ggtatcgtcc tctctcaacc
     5701 cgatgaatca cggtgttcgt tgttcgtcag atgtgctagt cagccgccgt atcctcggca
     5761 taggcgagcg gccggttgat ggattgatcg acgatctccc cgtccaccat gtaaacgacc
     5821 cggtcgcact cggcgccgat tccgcgatca tgcgtaacca gcacgaaggt caggccgttc
     5881 tcttcgttca gcttgcccat cagcgccatg atgtcggtcg ccgtcttcga gtccaacgca
     5941 ccggtcggct cgtccgccca gacaatcgcc gggtcattga ccagcgagcg cgcgatcgtc
     6001 acgcgctgcc gctgcccacc cgaaagctcg gccggacggt gattggcccg gttcgacagc
     6061 tccaccgtct ccagcgccgc cagcgccttc tgccgcgcat ccttcggcga aacgccggat
     6121 acgagcaacg gcaattcgac attctcgacc gcgctcagca ccggcagcag gttgtagacc
     6181 tggaagatga agcccatccg cttcgcccga tacgaggtcc gtttccgatc cttcatggtc
     6241 gaaaggtcct caccctcgag ccagattgcg ccggaatcga ttgtgtccag gcccgagagc
     6301 gtgttcagca gcgtcgtctt gccgctgcca gacggaccca tcacggccac catctcgccg
     6361 ctggtggcgc cgaagctgac gcccttgagt gcggcgacct tctgcgtccc ggtgttgtag
     6421 gtcttgacga catcccgggc ctcgatgatc aggttcgggt ccggcccggt gatgatcggc
     6481 gaggtggtgg cggtcgatcc tttgcggttc aaaagcgaaa gcatgtcggc actccttgtc
     6541 gttcctgatt cctgattcgc gggtgaactg cttgtctgca tctcattcct gttgtgaaga
     6601 cccgctctct cacgggtgtc tatagctact gtctattctg gccaccggct gaccgtctga
     6661 cgtcggaaag tcggccgaca cctgacgcgt acctgtgacc gatcagtcat ctccgtcaaa
     6721 gggcggaggc agggcgcgcg ctggtgaagt agagccacgc cccttccgtc caggcgcctt
     6781 gcatactgac cgagctcagc accggcacga cctgcaccag agacaacccg cgccggctca
     6841 tctcctggca ggcagcgtcc atctccgtcg ccagccgttg ccgccattcg tgctcgggct
     6901 cataccgcgg gaacattccg aaccatcgcc cggtgcgttg tcggctgctg ccgacgtaga
     6961 tcacccggct gtcacccatt gcgccgctca tcgatctccc tccctccagt cggatctggt
     7021 gccgccacaa gacacgtggc agcgtcgaac ctcactggct caattctggg tacccggagg
     7081 tcaactgcca tcggccgcgt gcctgaaatc ggtcgcggca aatctcaacc tcagagggag
     7141 aggcatctcc cccgcgagga tgatgagaac ggcaatcggg ttcgctatgc ttccaatatg
     7201 agtcgacttg accgtttacg cgccaccatc gagcagcatc cactcgcatt
//
LOCUS       DATAOZ010000213         8478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_93197, whole genome shotgun sequence.
ACCESSION   DATAOZ010000213 DATAOZ010000000
VERSION     DATAOZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93197"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VHR64_05645"
     CDS             <1..764
                     /locus_tag="VHR64_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3302346.1"
                     /translation="RQFVGLENYRRILTDDVVFRDALVNTFLFTVAAVAISLLLGLFY
                     AELVNHRFPGRGIVRTMLITPFLVMPVAGALGWKNMIMDPDFGVINWLLRAIQPGSGS
                     WNPVWLAHYPRQSIVIMLVWRWAPFMMLILLAGMQSIDEEVREAARVDGASPVQEFLS
                     ITLPHLYRFMQLGALLGTVYIVQEVDQIFMTTQGGPGTLTTNLPYYVYETAIRGSQVG
                     RGAAIGVVVVILTIIAMTLLLRLLDRMLRSSYAGS"
     gene            751..1662
                     /locus_tag="VHR64_05650"
     CDS             751..1662
                     /locus_tag="VHR64_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3302347.1"
                     /translation="MQAARPSIPFRRHPTGARKEGAPLAWAILAWVVGLIVFFPAFYI
                     VATGFKSEATAVQLPPKFIPIPGHPDLGWFNQFVFDPTLEQYREVKSRGFVPFFENSI
                     IASLLSTAIVLVLAVPAAYALALRPMKKWKDILFFFISTKFMPPAGIIIPLYIIFKNA
                     HLLNTIQGLVIMYTAMNLPIPIWMLRSFFEEVPRDVIDAARVDGAGIMREMIEILVPM
                     VGPGIAATAFLSMIFAWNEFFFAVNLVATEKAATVPLFMQKFITSEGLFWAKLSAAST
                     MAIAPVVVLGWISQRQLVRGLSMGAVK"
     gene            1824..2831
                     /locus_tag="VHR64_05655"
     CDS             1824..2831
                     /locus_tag="VHR64_05655"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX3302348.1"
                     /translation="MKAVVVEKPGVVRVEHVPEPVAGPKDVVVQVGACGICGTDIHII
                     DGEFPPTKYPIIIGHEFGGTVVGVGDEVVGVKVGDRVGVDPTLNCGECYFCQRGQGNL
                     CERWNAVGVGHHPGGFAECVAVPERAVYPIPEGMSFQSAALIEPVSCVVHGFHLITPR
                     PGDSYLVYGAGPMGLQNAQVARFYGAREVAIIDINPDRLEVARSFGFETLGQSLDDVK
                     SAAPRGFDIVIEATGRTKVAEIALNAVIRGGKLLLFGVCPPGETAAYEPFKIYNEELT
                     IIGSMAVLRSYGPAIDLLHAGAVDMEKMVTHTFPIDQFAEAVELVRKGGGLKVQIDPS
                     A"
     gene            3029..4045
                     /locus_tag="VHR64_05660"
     CDS             3029..4045
                     /locus_tag="VHR64_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX3302349.1"
                     /translation="MRQIVLERPQSIVMRDAPKPTPRPGDALVRSEQVGICGSDLHAY
                     HGKHPFITLPVVPGHEVVGTVEALGEGATGVQVGDRVVLEPNIICGECVHCRSGRYNL
                     CEKLTVVGCVGPLDGAMGDYFIAPAERLIPVDPLLDLTQAAMIEPLATGTHAVRVAGG
                     TNGKRVAVLGSGSIGLLTMQAAKAGGAAGIVVTDLSPAKRQRALDLGADAAFDPREPD
                     VVEAMRAQLGGPADVVFDCVAIQATMNQAIALAHKGGTVIVEGVPEADVTVPLAIIQD
                     REIRIQGTAMYTREDMLSAMNLIADGAVDAESLVTKTMPLEQAADAFAAADAGGEIKV
                     HLRV"
     gene            4251..5249
                     /locus_tag="VHR64_05665"
     CDS             4251..5249
                     /locus_tag="VHR64_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302350.1"
                     /translation="MTVDLMSRQLNRRQFAIGSAASLAALNFLPSHMRVASAQAAGDF
                     SAMGYPELAITMTDTGFENVPKSTEAGRYLLKLTSNLTSNNNGGAIGLFSPTAAGVSV
                     KAALKIIGAAGAAEATPAGGMPEATPMAGTPEAGGEQGQQIPLSIYRMYFAGGVAAAM
                     GETGEAVIDLIPGNYIVWGDDPSAPQKPSIMKVTGDFPKRVKDPTADITATLIDFAIT
                     MEGSLTAGKHIMKVQHHGAQPHFLEIEKGPDSMTKEQVMSTFSGMMSGTPPAEAIPES
                     EMQSIFFSPTQSIGTTTWQMIDLTDGTFLAACFFPTAGTGLPHAFNGMVDVFKVTG"
     gene            5458..6879
                     /locus_tag="VHR64_05670"
     CDS             5458..6879
                     /locus_tag="VHR64_05670"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEX3302351.1"
                     /translation="MPTLGNRSRSPLYVLIACLTLLALILPAGPQAAAQTPTAAGTPV
                     PIDDTWSPGQTVRIQNARGDLQVDAAVTELSDPHPSMTASRRGYRVVGVKLTLTNRGS
                     ATFEHAGNQLLLLDDAGLYVWDRQYLRVPREQTEQSLNWRELKLGESVSGWLYYEILS
                     AAKPAGLLYLGYESDPYAALLARFIAGHAGEAEAILDRSAATLGTIQVEQILTSFERT
                     DPSITPFRGMRAVALVVSVANTGDKTWDLQDSIILVDQFGLVYTRWYYDRSAASKGAY
                     PELKNRVHPGQTERGVIVFEIPADSSIEVIALVRNQEQFIVLGGGYASITATPEKYVA
                     TAGDEEWGSSAPGCEGIREWAEGSLAAMRMANEILSGVQADVTKATPEALRRSAASLR
                     YTASLQVGVVAPAKAREAQGDLIALLRTGADALDDAATRIENGESPEIVVDQLDAPKS
                     PFMLAANKALASSIAATVACPLD"
     gene            complement(6940..7596)
                     /locus_tag="VHR64_05675"
     CDS             complement(6940..7596)
                     /locus_tag="VHR64_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302352.1"
                     /translation="MTTQTFGMQHLIRSVLFAAIVAVLAVTSIQRAEANQSGPGVNDP
                     AQFQKALCEAAGGVGTIEVFRTVGSGLTDVQVNCKGGLLDGLRCDNHTDPVLGTICAF
                     LAPPPGGGQGTRPTTNVNEVPADQVLKPIDEVADPASTEQPSVNHPVTGDVTADPGTV
                     TDPIVSDESGTVVNQPVVADDSVDPVITGEPIIEPAPTVSPLVPVNPTQYDQPILIGQ
                     "
     gene            complement(7947..>8478)
                     /locus_tag="VHR64_05680"
     CDS             complement(7947..>8478)
                     /locus_tag="VHR64_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX3302353.1"
                     /translation="LLTALLLVTVGNALRSRTPTGEPISGLRLAYQIAVGIGLHNLGE
                     GLAIGSAFALGETALGVFLIVGFTLHNVTEGVGIAAPVVKEQRPSLWHFIGLAAVAGA
                     PAIIGTWIGAFTYSALWTTVFLAIGIGAIAQVIWEVGRLIQRSQARAGEPLFTWNTLG
                     GITAGIAVMYGTALLV"
BASE COUNT         1534 a         2797 c         2678 g         1469 t
ORIGIN      
        1 ggcgccagtt cgtcggcctc gagaactaca ggcggatcct gaccgacgac gtcgtcttcc
       61 gcgatgcgct ggtcaatacc ttcctcttca cggtcgccgc ggtggccatc tccctcctgc
      121 tcggcctttt ctatgcggag ctggtcaacc accgcttccc cggccgcggc atcgtccgca
      181 cgatgctgat caccccgttc ctggtcatgc cggtggccgg cgcactgggc tggaagaaca
      241 tgattatgga ccccgatttc ggggtgatca actggctcct gcgcgcgatc caacccggtt
      301 ccggctcatg gaatccggtc tggctggccc attacccgcg ccagtcgatc gtgatcatgc
      361 tcgtctggcg ctgggcgccc ttcatgatgc tgatcctgct ggccgggatg cagtcgatcg
      421 acgaggaagt gcgggaggcc gcccgcgtcg acggcgcctc gccggtccag gagttcctca
      481 gcatcaccct gccgcatctc tatcgattca tgcagctcgg cgcgctgctc ggcacggtct
      541 acatcgtcca ggaggtcgac cagatcttca tgacgaccca gggcggcccc ggcacgttga
      601 cgaccaacct tccctactac gtctacgaga cggcgatccg gggcagtcag gtcgggcgtg
      661 gcgcggcgat cggcgtcgtg gtggtgatcc tgacgatcat cgcgatgacg cttctgctgc
      721 gcctgctcga tcgcatgttg aggagcagct atgcaggcag ctagaccgtc catcccgttc
      781 cgccgccatc ccaccggcgc ccgcaaggaa ggcgcgccgc tggcctgggc gatcctcgcc
      841 tgggtcgtcg ggttgatcgt cttcttcccg gccttctata tcgtcgccac cggcttcaag
      901 tcggaagcca cggcggtgca gctgccgccc aagttcatcc cgatcccggg tcatccagat
      961 ctgggctggt tcaaccagtt cgtcttcgac ccgacgctgg agcaataccg ggaggtcaag
     1021 agccggggct tcgtgccgtt tttcgagaac tcgatcatcg cctcgctcct ctcgacggcg
     1081 atcgtgctgg tcctggcggt gccggcggcc tacgcgctgg cattgcggcc gatgaagaag
     1141 tggaaggaca tcctcttctt cttcatctcg accaagttca tgccgccagc cggtatcatc
     1201 atcccgctct acatcatctt caagaacgct catctgctga acacgatcca gggcctggtc
     1261 atcatgtaca cggccatgaa cctgccgatc ccaatctgga tgctgcgctc cttcttcgaa
     1321 gaggtgccgc gcgatgtcat cgacgccgcc cgggtcgacg gcgccgggat catgcgcgaa
     1381 atgatcgaga tcctggtgcc gatggtcggc cccgggatcg ccgcgaccgc gtttctgtcg
     1441 atgatcttcg cctggaacga gttcttcttc gcggtcaacc tggtcgcgac ggagaaggcg
     1501 gcgacggtgc cgctcttcat gcagaagttc atcacctcgg agggcctgtt ctgggccaaa
     1561 ctatcggccg cgagtacgat ggcgatcgcg ccagtcgtcg tcctcggctg gatctcgcaa
     1621 cgccagctcg tccgcggcct gtcaatggga gcagtgaaat gagtcgagca ggcgagaact
     1681 cgagaagtcg agtagcaggc gcggagacgg gaaggcgcga aggcgtggag ttatagagtt
     1741 caaggattag gggtcgggaa ccttcgcccg aaaacagaac cccgaaaacc caaaacctag
     1801 aacccaaaac ccaggagcta accatgaaag cagtcgtagt cgaaaagccc ggcgtggtcc
     1861 gggttgagca cgtgccggag ccggtggccg ggccgaagga cgtcgtcgtc caggtcggcg
     1921 cctgcggtat ctgcggcacg gatatccaca tcatcgatgg cgagttcccg ccgacgaagt
     1981 atccgatcat catcgggcac gagttcggcg gcaccgtcgt cggcgtcggc gatgaggtcg
     2041 tcggcgtcaa ggtcggcgat cgggtagggg tcgacccgac cctgaactgc ggcgagtgct
     2101 atttctgcca gcgtggccag ggcaacctct gcgagcgctg gaacgcggtc ggggtgggcc
     2161 accatccggg cgggttcgcc gagtgcgtag ccgtgccgga gcgcgctgtc tacccgatcc
     2221 cggaggggat gtccttccag tcggccgcgc tgatcgagcc ggtcagctgc gtggtccacg
     2281 gcttccacct gattaccccg cgtcccggcg acagctacct ggtctatggc gcggggccga
     2341 tgggcctgca gaacgcgcag gtcgcccgct tttacggcgc gcgcgaggtc gcgatcatcg
     2401 acatcaaccc ggatcggctg gaggtcgccc gctcgttcgg cttcgagacg ctcgggcagt
     2461 cgctcgacga cgtcaagtcg gccgcgccgc gcgggttcga tatcgtgatc gaggcaaccg
     2521 gcaggacgaa ggtcgccgag atcgcgctta acgcggtcat ccggggcggc aagctgctgc
     2581 tcttcggcgt ctgcccgccg ggcgagacgg cggcctacga accgttcaag atctacaacg
     2641 aggagctgac gatcatcggc tcgatggccg tgctccgcag ctatggccca gccatcgacc
     2701 tgctccacgc cggcgcggtc gacatggaga agatggtcac gcacaccttt ccgatcgacc
     2761 agttcgccga agcggtcgag ctggtccgca aaggcggcgg cctcaaggtg cagatcgacc
     2821 cttccgcata gggaacgtag tggcggggct ccgtacccgc ctggactcgc ccgacgcaat
     2881 gattcgcata tatcgaccgg ctccagattg atccggaaac cggtccgtct ctatgagaca
     2941 tccgattgcg ccactcaagg cgggtacgga gccccgccac tacgaatgga acgaggttct
     3001 ttgccatcgg ctgtttggag gattactgat gcggcaaatc gtgctggagc gcccgcaatc
     3061 gatcgtgatg cgggacgccc cgaaacccac cccgcggccg ggcgacgccc tcgtccgcag
     3121 cgagcaggtc ggcatctgcg gctcggactt gcacgcctat cacggcaagc acccgttcat
     3181 cacgctcccc gtcgtgccgg gacatgaggt ggtcggcacc gtcgaagcgc tcggcgaagg
     3241 cgcgaccggc gtccaggtcg gcgaccgggt cgtgctcgag ccgaacatca tctgcggcga
     3301 atgcgtccat tgccgctccg gccgctacaa cctctgcgag aaactgaccg tcgtcggctg
     3361 cgtcggcccg ctcgacggcg cgatgggcga ctacttcatc gccccggccg agcgcttgat
     3421 cccggtcgac ccgctgctcg acctgaccca ggcggcgatg atcgagccgc ttgcgaccgg
     3481 cacccacgcg gtgcgcgtgg cgggcggcac gaacggaaag cgggtcgcgg tcctcggcag
     3541 cggctcgatc ggtctgctga cgatgcaggc ggcgaaggcc ggcggcgcgg cgggaatagt
     3601 cgtcaccgat ctcagccccg ccaagcggca gcgcgccctc gatctcggcg cggacgcggc
     3661 attcgacccg cgtgaaccgg acgtcgtcga agccatgcgc gcgcagctcg gcggtccggc
     3721 cgatgtcgtc ttcgattgcg tcgcgatcca ggccacgatg aaccaggcga tcgccctcgc
     3781 gcacaagggc ggcaccgtga tcgtcgaagg cgtgcccgag gcggacgtaa ccgtaccgct
     3841 ggcgatcatc caggatcgcg agatccggat ccagggcacg gctatgtaca cccgcgagga
     3901 catgctctcg gcaatgaatc tgatcgcgga tggcgcggtc gacgccgagt cactggtcac
     3961 gaagaccatg ccgctcgagc aggcggccga tgcattcgcc gcggccgacg ccggcggcga
     4021 aatcaaggtc cacctcaggg tctagtcgac cgacattcac gtggcggaaa accggccggc
     4081 atcgcccgtc gtatatcggc gcgtgttggc aaccaccgct tcgatagcgc cacgaccgca
     4141 accgacgatt cctggatagg gcttccaacg cgagcccggg ccgtgcgccg cgccgaggtg
     4201 gaaggtctcg ccgctgccgt cctgttcgtc tcatgaggaa gggttcagcc atgaccgtcg
     4261 atctcatgtc cagacagctc aatcggcgtc agttcgcgat cggaagcgcc gcaagcctcg
     4321 cggcactgaa tttcctgccc tcccacatgc gagtcgcctc ggcccaggct gccggcgact
     4381 tctcggcgat gggctacccc gagcttgcga tcaccatgac cgacaccggc ttcgagaacg
     4441 tcccgaagtc gaccgaggcc gggcgctacc tgctcaagct gaccagcaat ctcaccagca
     4501 ataacaatgg cggcgcgatc ggtctctttt cgccgacggc agctggagtc agcgtcaagg
     4561 ccgcgctgaa gatcatcggc gctgccggcg cggccgaggc aacgcccgcg ggtggcatgc
     4621 ccgaagccac accgatggcc ggcacgccgg aagcgggcgg cgagcaaggc cagcaaattc
     4681 cactgtcgat ctaccggatg tatttcgccg gcggagtcgc tgccgccatg ggcgagacag
     4741 gcgaagcggt catcgatctc attcccggta actacatcgt ctggggtgat gacccatccg
     4801 caccccagaa gccgtcgatc atgaaggtca ccggcgactt cccgaagcgg gtcaaggatc
     4861 cgacggccga tatcacggcc acgctgatcg acttcgcgat caccatggag ggaagtctga
     4921 cggccggcaa gcacatcatg aaggtacagc atcacggcgc ccagccgcac tttctcgaaa
     4981 tcgagaaagg cccggactcg atgaccaagg agcaggtcat gtccaccttc tcggggatga
     5041 tgtctggcac gcctccggct gaagcgatcc cggaatcgga gatgcagtcg atcttcttca
     5101 gcccgaccca atcgatcggt accacgacct ggcagatgat cgacctgacc gatggcacgt
     5161 tccttgcggc ctgcttcttc ccgaccgccg ggaccggcct gccgcacgcc ttcaacggca
     5221 tggtcgacgt cttcaaggtc accggctaac cagcaaacca cctcacaccc cacggtccac
     5281 gcagagctcg agcgccgtgg accaacacaa cggcctccgg tcccccggag gccgtctctc
     5341 ttgtgcgctg cggcgatcgg ccggaatgcc gctacgccgc caatcaagac cgaagtcccg
     5401 tccgcaggct ggattccgca tcgacgataa gaaaagatcg ttgcggaagg gagaaccatg
     5461 cctacgttgg ggaatagatc gcgctcaccg ctctacgtcc tgattgcctg cctgacgtta
     5521 ctcgccttga tcctgccggc tggaccacag gcagccgccc aaacgccgac tgcggccggc
     5581 acgccggtgc cgatcgatga cacatggtct ccgggccaga cggtccgtat ccagaacgct
     5641 cgcggggacc tgcaggtgga cgcagcggtt accgagttgt cggaccctca tccgtccatg
     5701 actgcatccc gacgcgggta tcgcgtcgtt ggcgtaaagc tcacgctgac caaccgaggc
     5761 tcggcgacgt tcgagcacgc tgggaatcaa ctcctcctgc tcgacgacgc tgggctctat
     5821 gtgtgggata ggcagtacct gcgcgtcccg agggaacaga ccgagcaatc cctgaactgg
     5881 agggagctca agcttggcga atcggtttca ggctggctct actatgaaat tctgagcgca
     5941 gccaaaccgg ccggcctgct ctatctcggc tatgaatcgg atccctacgc tgcgctcctc
     6001 gcccggttca tagccggtca tgccggagag gctgaagcga tcctggaccg ctcggccgcg
     6061 acactcggga ccattcaggt tgagcaaatc ctgaccagct tcgaacgcac cgacccgtcc
     6121 atcacaccgt ttcgcggcat gcgggccgtg gcgctggtcg tctcggtcgc aaataccggc
     6181 gacaagacgt gggacctgca agattcaata atcctcgtcg atcaattcgg cctggtctac
     6241 acccggtggt attacgaccg cagcgccgcc tcgaagggcg cgtacccgga gctcaagaat
     6301 cgcgtccacc ctggccaaac cgagcggggc gtgatcgtct tcgagatccc ggccgactcc
     6361 tccatcgagg tcatcgcgct ggtccggaac caggaacaat tcattgtgct gggcggtgga
     6421 tacgcgtcta ttaccgctac gccggagaag tacgtcgcga ccgccggcga cgaggaatgg
     6481 ggtagctcgg cgcccggctg cgaagggatc cgcgaatggg ccgagggcag cctggctgcc
     6541 atgaggatgg ccaacgagat cctcagtggc gtccaggcag acgtcaccaa ggcgacccct
     6601 gaagcccttc gccgctccgc cgcttcatta cgctacaccg ccagcctcca ggtaggcgtt
     6661 gtagcgccgg ccaaggcgcg cgaggcccag ggagacttga tcgccttgct gcgcactggc
     6721 gccgacgcgc tcgacgatgc ggcgacgcgg atcgagaacg gggaatcgcc ggagatagtc
     6781 gttgatcaac tcgatgcgcc gaagtcaccg ttcatgctgg cggcaaataa agcccttgca
     6841 tcgtccatag cggctacggt cgcctgcccg ctcgactaag agcgtgcttc gtctcacggg
     6901 atgccgggtg gctcacccgg catcccgtgg ttaccgcatt tactggccga tcaggatcgg
     6961 ctggtcgtac tgagtggggt ttaccggcac gaggggagaa actgtcggcg ccggctcgat
     7021 gatcggctcc ccggtgatca ccgggtcaac ggaatcgtct gcaaccacgg gctgattgac
     7081 aaccgtgccg gattcatcgg acacgatcgg atcggtgact gtgcccggat cggccgtaac
     7141 atcgccggtc accgggtggt tgactgacgg ctgctcggtg gaagcaggat cggcgacctc
     7201 atcgatcggc ttcaggacct gatcagctgg aacctcattg acattggtgg tcggcctggt
     7261 cccctgacca ccgcccggag ggggcgccag gaaagcgcag atcgtgccga ggactggatc
     7321 ggtgtggttg tcacatcgca ggccgtccag cagcccgccc ttgcagttga cctgcacgtc
     7381 cgttaggccg gagccaaccg tgcggaagac ctcgatcgtg ccgacaccgc cggccgcctc
     7441 gcaaagcgcc ttctggaact gggcagggtc gttgactcct ggtccggatt ggttcgcctc
     7501 cgcccgctga atcgaggtga ccgcgaggac agcaacgatt gccgcgaaga gcacggaccg
     7561 gatcaggtgc tgcataccaa atgtctgcgt cgtcatctca ctgctccctg cctgctacac
     7621 cagcggttcg attgatgggt ccatcatcga ttcgcgggtg atacaggaaa tcagtgagat
     7681 cacgtaatta atacggggtt cggggttcgg ggttcgggaa cgagcgtaac ggtcaggcag
     7741 gtgcatcgcc acgatcaccg gcgcaccgcc acaaactctg tagggatttg ccgtggcaaa
     7801 tccgcgccag gatatcgtcc ccgggtccga tcccgatcgc gctttcggcg caaggcagcg
     7861 ctcggtccgg ccgcgagcgg atttgccacg gcaaatccct acaattgtgg tcgcaccttc
     7921 gcgccacaac tgctcgcctg ctcgccttac acaagcaacg ccgtgccgta catcaccgct
     7981 atcccggccg tgatgccgcc gagggtgttc caggtgaaga gcggctcgcc ggcgcgggcc
     8041 tggctgcgct ggatcaaccg gccgacctcc cagatcacct gcgcgatcgc gccgatgccg
     8101 atcgccagga agacggtcgt ccagagcgcc gaataggtga aggcgccgat ccaggtgccg
     8161 atgatcgccg gtgccccggc cactgccgcc aacccgataa agtgccacag gctcggccgc
     8221 tgctccttga cgaccggcgc ggcgatgccg acaccctcgg tcacgttgtg gagcgtgaag
     8281 ccgacgatca gaaagacgcc aagtgccgtc tcgccgagcg cgaaggcgct gccgatcgcc
     8341 agcccctcgc cgagattgtg cagtccgatc ccgaccgcga tctgataggc caaacgcagc
     8401 ccactgatcg gctcgccggt cggggtccgg ctgcgcaggg cgttgccgac cgtgaccagg
     8461 agcaaggcag tcaaaagg
//
LOCUS       DATAOZ010000214         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7457482, whole genome shotgun sequence.
ACCESSION   DATAOZ010000214 DATAOZ010000000
VERSION     DATAOZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7457482"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VHR64_05685"
     CDS             complement(<1..684)
                     /locus_tag="VHR64_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302354.1"
                     /translation="MATERNPKKPDVNSFDGPLRDRIPRYVISLPERLTRAAIAIVGG
                     AVYETSNVALPRALRQSKLYQVTIDRLLRILIEWVGDVRDVYQDEETPVQQLAARKFT
                     GNVLEVASIFAVGFSPLWVLAAASDVMGGSKAYLRALVNELEAAGHLPQNTDIASYEE
                     LLSRLETGSGVMADAIDVPPMSLQEARASFDALRRQASDLPPAEDLATLFREMQSTAR
                     RERRSLTDVS"
     gene            953..2137
                     /locus_tag="VHR64_05690"
     CDS             953..2137
                     /locus_tag="VHR64_05690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3302355.1"
                     /translation="MSLIADVVVIGGGVNGTSTAFHLAKRGVSVTLLERSSLAAGSTG
                     KSGALVRMHYTNPHDSALAQQSLPYFQNWADLVGAGDPGYRQVGVVRLVQPRYDERLR
                     TNVAMLQGLGVNTEVISPDDLRAIDPGVQTGDITCAAWEPESGYADPAATAYGFAEAA
                     RGLGASVHEGVEATAIETEGERVTGVITPLGRIATETVVAAPGARAIALLAPLGLDYG
                     LQPNRVQIVIMRRTADDGPSHPVYIDGPNNTWLRPEGDFGTLCGIGRDQLGVNPDLYD
                     EGVDFDYIVDSRTRCAARRPALANSFMRGGWAGIITMSPDGHAIIDELAPYRGLFGIL
                     GDSGTNFKTAPAIGKCVAEWIAKGASQTVDLRPFRASRFAEGQFFAGEHEYGDSPLDV
                     FR"
     gene            2214..>3661
                     /locus_tag="VHR64_05695"
     CDS             2214..>3661
                     /locus_tag="VHR64_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3302356.1"
                     /translation="MIRHHDDLVRSMEQVRDGRLSRRAFLGAAVAAGFSASIAERLLN
                     HGATLAAPARAVARQDEPPKGGQVIIGLSQEPTIFNPLKSTLEVDRGVQFAIFDSLWR
                     IDQEAKLIPNLATEIPSIENGGISEDGLTYTFKLRDDAAWHDGEPFTAADVVFSHETI
                     MNPDFVPGTRIGHDQVAEISAPDDTTVTMTLKAPYAPFLIVWADTYLVPEHILAEVED
                     LNTAEFNSTAPIGTGPFTFAERVAGDHITLQANPNYHGPGPYLDTAIYKYVPDLTVLY
                     TQFKTGEVDATGLQGITADNVAEAKTLEGKVIHSEPTGFVEFIYMNHGNPVFQDKAVR
                     EALYAAMDKENIINTVYYGIHGPIESYLPQQSWAFNPDLPAQVFDIAAANATLDEAGW
                     APGDDGIRQKDGVKLSFTNSTTAGNKVREQAQQYLQQTWREAGIDMQIENMPAAVIWG
                     DYFNKSQYDSVMVGWTNPPDPDGTSRFHSDYIP"
BASE COUNT          709 a         1172 c         1159 g          621 t
ORIGIN      
        1 cgagacatcg gtcaatgacc ggcgttctcg ccgcgcggtc gattgcatct cccggaagag
       61 cgtcgccaaa tcctcggccg gaggcagatc actcgcctgc cggcgcagag cgtcgaacga
      121 ggcgcgagct tcctgcaacg acatcggggg gacgtcgatc gcatctgcca tcaccccgga
      181 gccggtctcg agacgactca acaactcctc ataggacgcg atgtccgtgt tctgagggag
      241 atgacccgcc gcttccagct cgttcaccag cgctcgcagg taggccttcg agccgcccat
      301 gacgtcggac gcggcggcca gcacccagag cggcgagaag ccgacggcga agatgctggc
      361 aacctccagc acattgccgg tgaatttgcg cgcggccagc tgttggacgg gggtctcttc
      421 gtcctgatag acgtcgcgca catcgccgac ccactcgatc aggatgcgca gaaggcggtc
      481 gatggttacc tgatagagct tggattggcg tagcgcacgc ggcagtgcga cattgcttgt
      541 ctcgtagacc gcgccgccga cgatggcgat cgcggcgcgc gtcagtcgct ccggcagcga
      601 gatcacgtag cgcgggatgc gatcccgcaa tggtccatcg aaggagttga catcaggctt
      661 cttcgggttc cgctcagtcg ccatagcggg aacgatacga gtggacctgg cgagaagtcg
      721 agatcgggtg aaacaggtag acacctcgtg cacatgcaat tcgcaaacgt tcctgcggcc
      781 ttccctcagg ccctgaagcg gatatcgttg acgcaaggcg gaatcgtcgc ccagtgacga
      841 atccttccgt atttcgtgct ggtgcctcgc tgacaagacc cgcaattatc ggctaggatg
      901 atcatattga tcagcttcca ccccctggcc cctggttgga aaggaggatc ccgtgagtct
      961 gatcgccgac gtcgtcgtca tcggcggtgg tgtcaatggc acgagcacgg cttttcacct
     1021 cgcaaagcga ggcgtgagcg tcaccctcct cgagcggtct tcgctggcgg cgggctcgac
     1081 cggcaagtca ggggcgctcg tgcggatgca ctacacgaat ccccacgatt cagcgctggc
     1141 ccagcagagt ctcccctact ttcagaactg ggcggacctc gtcggggcag gagatcccgg
     1201 ctatcgccag gtgggcgtcg tccggctcgt gcagccccga tatgacgagc gtctccgcac
     1261 caacgtcgcg atgctgcaag ggttgggggt gaacaccgag gtgatctccc cggacgacct
     1321 ccgtgcgatc gacccgggcg tgcagaccgg cgatatcacc tgcgccgcct gggagccaga
     1381 atcgggctac gccgacccgg cggcaaccgc ttatggcttt gccgaagcgg cccgcgggct
     1441 tggtgcgagc gtccacgagg gggtcgaggc gacggcaatc gagacggagg gcgagcgagt
     1501 cacgggagtc atcacgccgc tcgggcgcat tgccactgag acggtcgtcg ctgcgccggg
     1561 cgcccgcgcc atcgcactgc tggcgccgct cggactcgac tatgggctgc agccgaaccg
     1621 ggtgcagatc gtgatcatgc gccgcaccgc ggatgatggg ccgtcgcacc ccgtctacat
     1681 cgacggaccg aacaacactt ggctgcgccc ggaaggagat ttcggcacgc tctgcggcat
     1741 cggccgcgac cagctcggtg tcaaccccga tctctacgat gaaggggtcg atttcgacta
     1801 catcgtcgat tcgcgcaccc gctgcgcggc gcgccggccc gctctggcca acagcttcat
     1861 gcgtggcggc tgggcgggga tcattaccat gagccctgac ggtcacgcga ttatcgacga
     1921 gctcgcgccg taccgggggt tgttcgggat cctgggcgac agcggcacca acttcaagac
     1981 cgctcccgcc atcggcaaat gcgtggcgga gtggattgcg aagggtgcgt cgcagacggt
     2041 cgatctgcgg cccttccgcg cctctcgctt cgcggagggg cagtttttcg cgggcgagca
     2101 tgagtacggt gacagcccgc tcgatgtctt tcgctaagcg ccttatcaag gccgttcaca
     2161 tccgggcggg gagcttgctt gggaatcccg ggagagagga taaggagaag acgatgatcc
     2221 gtcaccacga cgatctagtt cgctcgatgg agcaggtccg cgacggccgc ctgagccgcc
     2281 gcgcatttct cggtgcggcc gtggccgcgg gattctcggc atcaattgcg gagcggctgt
     2341 tgaaccacgg cgccacgttg gcagcgccag ccagggcggt cgcccgccag gatgagccgc
     2401 cgaagggcgg tcaggtgatc attgggctct cgcaggaacc gaccatcttc aatccgctca
     2461 aatcgaccct ggaggtcgat cgcggcgtgc agttcgccat tttcgacagc ctctggcgga
     2521 tcgaccagga agcgaagctg atcccgaatc tggcgaccga gatcccgagt atcgaaaacg
     2581 gtggaatctc agaagacggt ctgacctata cgttcaaatt acgtgacgac gccgcttggc
     2641 acgacggtga gccgttcacg gcggcggacg tcgtcttcag ccacgagacg atcatgaatc
     2701 ccgatttcgt ccctggcact cgcatcgggc acgatcaggt ggcggaaatc tcggccccgg
     2761 acgacaccac cgtgacaatg acgctcaaag cgccgtacgc gccattcctg atcgtctggg
     2821 ccgacaccta cctcgtcccc gagcacatcc tggcagaggt ggaggatctc aatacggccg
     2881 agttcaactc gacggcgcca atcgggaccg ggccattcac cttcgctgag cgggtggcgg
     2941 gcgatcacat cacactgcag gcaaacccga actaccacgg tcccggaccg tacctcgata
     3001 ccgccatcta caagtacgtg cccgacctga ccgttctcta cacccagttc aagacgggcg
     3061 aggtcgatgc cactggtctc caagggatca ccgccgataa cgtcgccgag gcgaagacgt
     3121 tggagggaaa ggtcatccac agcgagccga caggcttcgt cgagttcatc tacatgaacc
     3181 acggcaaccc ggtcttccag gacaaggcgg tgcgtgaggc actctacgcg gcgatggaca
     3241 aagaaaacat catcaacacc gtctactacg ggatccatgg cccgatcgag tcatacctgc
     3301 cgcagcagtc atgggccttc aaccccgatc tcccggcaca ggtcttcgac atcgcagcgg
     3361 ccaacgcgac cctggatgag gcgggctggg cgccgggcga cgatggcatc cgccagaagg
     3421 atggcgtcaa gctcagcttc actaactcga cgacggccgg caacaaggtc cgcgagcaag
     3481 cgcagcagta cctgcagcag acgtggcgcg aggccgggat cgacatgcag atcgagaaca
     3541 tgcccgcggc ggtgatctgg ggcgactact tcaacaagtc gcagtacgac tcggtgatgg
     3601 ttggctggac caacccgccc gacccggatg gcacaagccg cttccactcc gactacatcc
     3661 c
//
LOCUS       DATAOZ010000215         5735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2722538, whole genome shotgun sequence.
ACCESSION   DATAOZ010000215 DATAOZ010000000
VERSION     DATAOZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2722538"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VHR64_05700"
     CDS             complement(<1..505)
                     /locus_tag="VHR64_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3302357.1"
                     /translation="MHVTIFGTGYVGLVTGACLAEVGNDVLCVDVNAERIAGLNDGIV
                     PIYEPGLEEMVLRNRRAGRLQFTTDGAAAVNACDVIFIAVGTPPLPDGSSDLQYVDSV
                     ARTIGQHLTAPKVIVDKSTVPVGTADRVRQIIGEELAARSIELAIDVVSNPEFLKEGA
                     AIEDFMKP"
     gene            complement(622..1557)
                     /locus_tag="VHR64_05705"
     CDS             complement(622..1557)
                     /locus_tag="VHR64_05705"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3302358.1"
                     /translation="MWLVTGGAGFIGSHIVHRLVRDGQPVRVIDNFSTGNLDKLAEVR
                     SQIELIEGDIRDEATVRTAMQDVTVVLHHAAQPSVPRSVEDPAGTYAVNLTGTMHLLD
                     AARDAGVRRFVLASTSAIYGEDPTSPKSEALMPRPISPYASSKLGAEQLCAVYHRCYG
                     LECVALRYFNVYGPRQDPNSAYAAVIPRMVDLLRRGERPIVYGDGEQTRDFIFVGDVV
                     EANLRAAVAGVEPGGVYNIASGRPTSLNQLLAVLSAQFGVAPDADYQTERAGDIRHSL
                     ADPSLAATDLGFTARTTLEDGLAMTLAGCEMVGAA"
     gene            complement(1741..2043)
                     /locus_tag="VHR64_05710"
     CDS             complement(1741..2043)
                     /locus_tag="VHR64_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302359.1"
                     /translation="MDDSTVQVNPDPFETIESLARTVAHLALQLTISQIQLRAMGSAI
                     EQAGVVGSDDVSRATANIARRNAGRFLRENLGPELTEMIDVTDLERQIVAYLSSGN"
     gene            2165..3106
                     /locus_tag="VHR64_05715"
     CDS             2165..3106
                     /locus_tag="VHR64_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302360.1"
                     /translation="MHVGFSFWSARAVRFLVLASLIAGVVSFGGSVSTAGAADCALPG
                     PIGSLASTPSAGATPLSAPPQATPVSTRDELKAGLTNLVQAVSACRSNGDFETMSKLV
                     TEKYLGQVYGGGPRMSRDTFLAIADSLPTPKVRFRGFDDLSVTGPGEARANVLLIVGN
                     QLLFQRLTFIEEPKRPGNWLIDGVEPLRVKAPRDHATLRLTITGNRYSPDALTADSGT
                     VNIHVSNSDKEDHELLVLKLADGVTTNDLLTSEGPGFPEGVTYLGQVTIPAHGSGSMV
                     LVGLKTGTYSVVDLLPSDNGVPHLAVGMSATLTVTGQ"
     gene            3157..4890
                     /locus_tag="VHR64_05720"
     CDS             3157..4890
                     /locus_tag="VHR64_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX3302361.1"
                     /translation="MRLLLEMTLTVPEDQLPGAEGPRAPFASTLPTVRLLKDESSAHP
                     FATTIAAAWSCYGAKPARVENVLKLVHGPGPANQSAETAADRADRRDRALRLYADLFA
                     AGHHTTFQHANFTFVLDNVSRLAIWSFFHAHPHYNSEQVSQRYREVSGAVMTTPDLPA
                     PQLEIYRAAINQSLEGYRRLTEILSVDALAGYTQVFPSRVKATGEEAEAKVASAIQKK
                     AQEVARYVLPLATPAHLYHTVNGLTLLRYLVLSSQPDVPAEVRYIVQRMVEEVLAVDP
                     YFLGAPGYPLNLRPLAEEETLESQMYARWHETVGQRADDAERFCTDFDGQLGRWQSSR
                     LAAWNPDGEQLLADAVRTVLGASDMPDDDALAAVLSGADNPYLGHALYLGMNSKLMQT
                     LNHVPFTFQKRISGAEDAQNQRHRGTLASRPVLQAHLRRAPDVITPWAIVRNSEALAV
                     YQQTVQALWDAKNALLDGGAPVETALYLLPNSHRVRFYESGTLMTYYWKWIKRLCYDA
                     QREIFETAVEEVAQVREVYPHIGRYVDGPPCVMRSRTETRPFCPEGERYCGIPVWRNY
                     QFETLEERRVL"
     gene            4906..5301
                     /locus_tag="VHR64_05725"
     CDS             4906..5301
                     /locus_tag="VHR64_05725"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX3302362.1"
                     /translation="MTWLWIALAILTFVLVFLITILIQVRRSGMTPAEATTWLREMGV
                     DLVKLPGRLRRVAADPRTPRRARWWLLGLAIYIASPIDPIPDFLPVIGYLDEIVLVPL
                     ILRHVRKMIPDEVWLEQFPPRPDPADPSI"
     gene            5396..5707
                     /locus_tag="VHR64_05730"
     CDS             5396..5707
                     /locus_tag="VHR64_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX3302363.1"
                     /translation="MQTFMVTSTAGPNRDLTRDTRQQPHWDDHAAFIDGLVEDGFIFL
                     GGPLVDEGGAVIVVQAEDEASVRSVLANDPWYQHGILTLASIKRWQIFIDERAATIAT
                     T"
BASE COUNT          987 a         1941 c         1812 g          995 t
ORIGIN      
        1 ccggcttcat gaagtcttcg atcgccgcgc cttccttgag gaactcggga ttggagacga
       61 cgtcgatcgc caactcgatg ctgcgcgccg ccagctcctc gccgatgatc tgacgaacgc
      121 gatcggccgt gccgaccgga accgtcgact tgtcgacgat gaccttcggc gcggtcaggt
      181 gctgtccgat cgtgcgcgcc acgctgtcga cgtactgcag atcggacgag ccgtccggca
      241 gcggcggcgt gccgacggca atgaagatca cgtcacaggc gttgaccgcc gcggcgccgt
      301 cggtcgtgaa ctggagcctg ccggcgcggc gattgcggag gaccatctcc tcgagacccg
      361 gctcatagat cggcacgatg ccgtcgttga gcccggcgat tcgctcggcg ttgacatcga
      421 cgcagaggac gtcattgcct acctcggcga ggcaggcgcc cgtaacgagg ccgacgtatc
      481 cagttccgaa gatcgtgacg tgcacggttc ctcctacacc cggggagatg cggttccgac
      541 cggggaaatc atcagtggtg cgggacgatc accggaccgg gatcatcaga gggtcagcct
      601 agcgggtgga tggggcaatc atcaagcagc gccgaccatc tcgcaccccg cgagggtcat
      661 tgccaggcca tcttcgagcg tggttcgggc agtgaagccc aggtcggtag ccgccagcga
      721 cggatctgcc agggaatgac gaatgtcccc ggcccgctcc gtctggtagt ccgcatccgg
      781 cgcaacgccg aattgggccg acagcaccgc cagcagctga ttgagcgatg tcggtcgtcc
      841 cgaggcgatg ttgtagaccc cgcccggctc gacgccggcc acggctgcgc gcaagttggc
      901 ctcgaccacg tcgcccacga agatgaagtc gcgcgtctgc tcgccatccc cgtagacgat
      961 cggtcgctcc ccccgccgga gcagatcgac catgcggggg atcaccgccg cgtaggccga
     1021 attcggatcc tggcgcggtc cgtagacgtt gaagtaccgc agcgcgacgc attccaggcc
     1081 ataacagcga tggtagaccg cgcagagctg ctcggcgccg agcttcgacg atgcgtaggg
     1141 cgatatgggc cgcggcatca gggcttcgga cttcggcgag gtcggatctt cgccgtagat
     1201 cgccgaggtc gaggccagca cgaagcggcg cacgccggca tcacgcgcgg cgtcgagcag
     1261 atgcatcgtg ccggtcaggt tcacggcgta ggtgccggcc ggatcctcga ccgagcgcgg
     1321 caccgagggc tgtgctgcgt ggtggagcac gaccgtaaca tcctgcatcg cggtccgtac
     1381 cgttgcttcg tcccggatat cgccctcgat cagctcgatc tgcgaccgga cctcagccag
     1441 tttgtcgagg ttcccagtcg agaagttgtc gatcacgcgc acgggttgac cgtcgcggac
     1501 gagccggtga acgatatggg agccgatgaa accggccccg ccggtgacga gccacattgc
     1561 agatccacct gccagattga gtgcgagtcg aggcgacgtt gccagccggg gcgcgcccgg
     1621 catttccacc ttagcacgcc cggatgaacc aatcgggtgg gggaagcgcc ccggatacat
     1681 tagtcgcccg gaaatgggac tgccggttcg tccaggtcgt gattctgttg cggcggcgga
     1741 ttaattaccc gagctgaggt acgccacgat ctgccgctcg aggtcggtca cgtcgatcat
     1801 ctccgtcagc tccggcccaa gattctcgcg caggaagcgg cctgcattgc ggcgcgcaat
     1861 gttcgcggtg gcacggctga cgtcgtcgct gccgacgacg cccgcctgct cgatggccga
     1921 gcccatcgcc cggagctgga tctgcgagat cgtcaactgc agcgcgaggt gcgcgaccgt
     1981 ccgcgccagc gactcgatcg tctcgaatgg atccgggttg acctgtacag ttgagtcgtc
     2041 catgacctgc tcgtgtattg ctgcccctgc tagacttgga tttccgcatt ccggtgaatc
     2101 caattcgccg aaccccgcga tcatacattg ggtagttggt gaatgagcgc aggtgttgct
     2161 tgtcatgcac gtggggttca gcttctggtc ggcgcgcgcg gttcgtttcc tggtcctggc
     2221 gagtctgatt gccggcgtcg tcagctttgg cggttcggtg tcaacggccg gcgcggccga
     2281 ttgcgcactg ccgggtccaa tcggcagcct ggcatcgacg ccgtcagccg gcgcgacacc
     2341 cctctcggcg ccaccgcagg cgacgccggt ctcgacccgg gatgagctca aggctggcct
     2401 cacgaatctc gtccaggccg tctcggcctg ccgatcgaac ggggatttcg agaccatgtc
     2461 gaagctggtc accgagaaat acctcggtca ggtgtacggc ggcggacccc ggatgtcgcg
     2521 cgacaccttc ctggcgatcg cggatagcct cccgaccccg aaggtccggt tccgcgggtt
     2581 cgatgatctg tccgtaaccg gccccggcga ggcgcgcgcg aacgtgctgc tgatcgtcgg
     2641 caaccaactc ctcttccagc gcctcacttt catcgaggag ccaaagcgcc ccggaaactg
     2701 gctcatcgat ggcgtcgagc cgctccgcgt caaggcgccg cgggaccatg cgaccctccg
     2761 cttgaccata accggtaatc ggtacagccc cgacgcgctg acggccgaca gcgggacggt
     2821 caacatccat gtcagcaact cggacaagga agaccacgag ctgctcgttc tcaagctcgc
     2881 cgacggcgtc acgacgaatg acctgctgac ctcggagggc cccggcttcc cggagggggt
     2941 gacctatctc ggccaggtga caatcccggc gcacggcagc ggcagcatgg tgctcgttgg
     3001 tctcaaaacg ggcacgtact ccgtcgtcga cttgttaccc tcggacaatg gagtgccgca
     3061 tttggctgtc gggatgtcag ccacattgac cgtcacgggg cagtaacggc cggttggtat
     3121 gcttgtcgga gccgggcgcc gcgcccgggt ccacgcgtga gattgctgct cgagatgacg
     3181 ttgaccgttc ctgaagacca attgcccggc gccgaaggtc cgcgcgctcc gttcgcctcc
     3241 accttgccga cggttcggct gctcaaggac gagtcgtctg cgcacccgtt cgcgaccacg
     3301 atcgcggcgg cttggtcctg ctatggggcg aaaccggcca gggtcgaaaa tgtgctcaag
     3361 ctggtccacg gtcccggtcc ggccaaccag agcgcggaga cggccgcgga tcgggccgat
     3421 cggcgggatc gggcgcttcg actctatgcg gatctcttcg cggccgggca tcacaccacc
     3481 tttcagcacg ccaacttcac cttcgtgctc gacaatgtca gccggctcgc gatctggtcc
     3541 ttcttccacg cgcacccgca ttacaactcc gagcaggtct cccagcgcta ccgagaggtc
     3601 agcggagcgg tcatgaccac gcccgacctg cccgcgccgc agctcgagat ctaccgggcg
     3661 gcaatcaacc aaagcctcga gggataccgg cggctgaccg agatcctgtc ggtcgacgcc
     3721 ctggccggct acacgcaggt cttcccgtcg cgcgtcaagg cgaccggcga ggaggcggag
     3781 gccaaggtcg cctcggccat ccagaagaag gcgcaggagg tcgcgcggta cgtgctgccg
     3841 ctggccacgc cggcgcacct ctaccacacc gtcaacggcc tgaccctgct gcgctacctc
     3901 gtcctttcca gccagcccga cgtgccggct gaggtccgtt acatcgtcca gcgcatggtc
     3961 gaagaggtgc tggctgtcga tccgtacttc ctcggcgcgc ccgggtaccc gctcaacctg
     4021 cggccactcg cggaggagga gacgctcgaa tcgcagatgt acgcccgctg gcacgagaca
     4081 gttggtcagc gcgcggacga tgccgagcgc ttctgtaccg atttcgatgg ccagctcggt
     4141 cgctggcaga gcagtcgact ggccgcctgg aacccggatg gcgagcaact cctggccgac
     4201 gcagtccgga ctgtcctcgg cgcctccgac atgccggacg atgacgctct cgccgccgtc
     4261 ctgagcggtg ccgataaccc atatctcggc catgcactct acctgggcat gaactccaag
     4321 ctgatgcaga cgctcaatca tgttccgttc accttccaga agcggatcag cggggccgag
     4381 gatgcgcaga accagcgcca tcggggcacc ctcgcctccc gcccggttct gcaggcgcat
     4441 ctccgtcgcg cgcccgacgt gatcacgcca tgggcgatcg tgcgaaattc cgaggcactt
     4501 gccgtctacc aacagaccgt gcaggcgctc tgggacgcga agaacgccct gctcgacggc
     4561 ggcgcgccgg tcgagaccgc gctctacctc ctgccgaaca gccaccgggt ccgcttctac
     4621 gagagcggca cgctgatgac ctactactgg aagtggatca agcgcctctg ttacgacgcc
     4681 cagcgcgaga tcttcgagac ggccgtcgag gaggtggcgc aggtccgcga ggtctacccg
     4741 cacatcggcc gctatgtcga tggcccgccc tgcgtgatgc gcagccggac cgagacccgc
     4801 ccattctgcc cggaaggcga gcgctactgc ggcatcccgg tctggcgcaa ctaccagttc
     4861 gagacgctgg aagagcgtcg cgttctctag cgatcaggca gtccattgac ctggctctgg
     4921 atcgcgcttg ccatcctgac gttcgtgctt gtgtttctca tcaccatatt gattcaggtg
     4981 cggcgatcgg gcatgacgcc ggccgaagcc acgacctggc tgcgcgagat gggcgttgac
     5041 ctggtcaaac tgccgggccg gcttcgccgg gtcgcggccg atcctcgcac gccccggcga
     5101 gcgcgctggt ggctgctcgg cctcgccatc tacatcgcca gcccgatcga ccccatcccc
     5161 gatttccttc cggtcatcgg ctacctcgac gagatcgtcc tcgtccccct gatcctccgc
     5221 cacgtccgca agatgattcc ggacgaggtt tggctggaac agttcccccc tcgaccggac
     5281 ccggccgatc catcgatctg agcaacagaa atgcggacag ttcctttccg catccgtggc
     5341 taggttctat tcaacgcacg cgggattcgc tgatacttcg cgccctggag acgaaatgca
     5401 aacgttcatg gtcacatcga ccgccggccc gaaccgtgac ctgaccaggg acacccggca
     5461 gcaaccacac tgggacgatc acgccgcctt catcgacggc cttgtcgaag acggcttcat
     5521 cttcctcggt ggcccgctgg tcgacgaggg cggcgctgtg atcgtcgttc aagcggagga
     5581 cgaggccagc gtacgctctg tcctcgccaa cgacccctgg taccagcacg gcatcctcac
     5641 cctcgccagc atcaaacgct ggcaaatctt catcgacgag cgggctgcga ccatcgcgac
     5701 gacatagcga ggcacaaccg gaccgcccag gacga
//
LOCUS       DATAOZ010000216         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2985321, whole genome shotgun sequence.
ACCESSION   DATAOZ010000216 DATAOZ010000000
VERSION     DATAOZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2985321"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VHR64_05735"
     CDS             complement(<1..902)
                     /locus_tag="VHR64_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302364.1"
                     /translation="MRNLLGRLNARSTPDLSQIADAWQTPLAGRDRLAQITQLYRTMT
                     RLPVVRARWDSLDPDQQAVVSALIAAGEKGRTIEDLSAELGAAPDLVRTMCVDLFDQG
                     IVSYEGNASTLPIGEKPRLYVPIELANAIRTVIREVEAGDVSGRSFPELIQSKDERDL
                     FEAAGSWGVEVIAGVTTRAQLIEGLTRAAAAGASRHAQVDKLGSEVRAIWEKLRAVPP
                     GTPVPVDQLVGTGNERTTYGRRNAINELEDRLLVWPTVLEDGVRALFVPAEIANASTS
                     GDLEIARPKPVSVVGSEPPYRPPGP"
     gene            complement(957..1403)
                     /gene="crcB"
                     /locus_tag="VHR64_05740"
     CDS             complement(957..1403)
                     /gene="crcB"
                     /locus_tag="VHR64_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX3302365.1"
                     /translation="MIAEDEATTEETRHASIADGWTGVVPTMLAIGIGGIIGANLRWK
                     IGVWAADQWATPFPWGTLLINLTGSFVLGLYLTLVTERFTGRPLTRLMFATGLLGAYT
                     TFSTFAYETVRLLQHGQIWTALAYVAASLALGLAACTGGIATGRAI"
     gene            complement(1400..1786)
                     /gene="crcB"
                     /locus_tag="VHR64_05745"
     CDS             complement(1400..1786)
                     /gene="crcB"
                     /locus_tag="VHR64_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX3302366.1"
                     /translation="MSAFLLVSAGGFVGANVRYGISVWSARRFGTGFPVGTMIANIGG
                     SFLIGLVLGLLAGWFDDDPQMRLLLATGFLGAETTFSTWTWESMALIKEGLIGRAARN
                     LFGSVALGMIAVTAGLLLAYFLTDVL"
     gene            1936..2286
                     /locus_tag="VHR64_05750"
     CDS             1936..2286
                     /locus_tag="VHR64_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX3302367.1"
                     /translation="MDHQLDGRGVRVRAYFGERDHHHGKPLWSALLEFLRAHGAAGAT
                     VTRGIAGYGAHSKIHAASIVDLSSDLPLVLEWIDTEERAGSLLPELEEMLQGGLITTD
                     PVTIIRYQPHTDRA"
     gene            2375..>2810
                     /locus_tag="VHR64_05755"
     CDS             2375..>2810
                     /locus_tag="VHR64_05755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3302368.1"
                     /translation="MTAVATHRHDVIIVGAGVAGCSAAYRLAQAGRNVLVLEKRGIAS
                     GASGRNGGMTGVGSAMHADAGPAVYSITSANLALLKQLPDELETDFELRLPGTLNVAM
                     TEDHWAYLNGSVEEQRAGGLDVTMIDGYEARQMMPALTEKAIG"
BASE COUNT          488 a          976 c          889 g          457 t
ORIGIN      
        1 ggacctggcg gccgataggg tggctccgag ccgaccacgc tgaccggctt cggtcgcgcg
       61 atctccagat cgccgcttgt gctggcgttg gcgatctcag ccggcacgaa aagcgcgcgc
      121 acgccgtcct ctagcaccgt cggccagacc agcaaccggt cctcgagctc gttgatcgca
      181 ttccgccggc cgtatgtcgt ccgctcgttg ccggtgccga ccagttgatc gaccggcacc
      241 ggcgtgcccg gcggcacggc ccgcaacttc tcccagatcg cgcgcacttc gctgcccagc
      301 ttgtcgacct gggcatggcg ggacgcgccg gcggcagcgg ccctggtcaa gccttcgatg
      361 agctgagcgc gggtcgtcac gccggcaata acctcgactc cccacgaccc cgcggcttcg
      421 aacagatccc gctcgtcctt ggactgaatc aattccggga aggaccgacc gctgacatcg
      481 cctgcttcta cctcgcggat caccgtccgg atcgcattgg ccagctcgat cggcacgtag
      541 aggcgcggct tctcgccgat cggcagggtt gaggcattgc cttcgtacga gacgatgccc
      601 tggtcgaaga ggtcgacgca catcgtccgg accaggtcag gagcggctcc aagttcagcg
      661 ctcagatcct caatcgtccg ccccttttcg ccggcagcga tcaacgcgga gaccaccgcc
      721 tgctgatccg gatcgagcga gtcccaccgg gcacgaacga ccggcagccg ggtcatcgtg
      781 cgatagagct gggtgatctg ggccaggcgg tcccgcccgg ccagtggcgt ttgccaggcg
      841 tcggctattt gactgagatc cggagtcgat cgggcgttga gccgaccgag cagattgcgc
      901 atacccaccc tgtcactaca taaagcgcat gataccgccg acgcgatctc cgccgctcag
      961 atcgcccgcc cggtcgcgat cccgcccgtg caggcggcga gtccgagcgc gagactggcc
     1021 gccacgtagg cgagcgcggt ccagatttgc ccatgctgga ggagtcgcac cgtctcgtag
     1081 gcgaaggtcg aaaaggtggt gtaggcgccg aggaggccgg tcgcgaacat gagacgggtc
     1141 agcggcctgc cagtgaatcg ctcagtcacc agtgtcagat agagaccaag gacgaaactg
     1201 ccggtcaggt taatcagcag cgtgccccat gggaagggcg tcgcccattg atcggcggcc
     1261 cacacgccaa ttttccagcg caggttggcg ccgatgatcc cgccgatccc gatcgccagc
     1321 atcgtcggga cgacgcctgt ccagccgtcg gcaattgacg catggcgcgt ctcctccgta
     1381 gtggcctcgt cctcggcgat cacaggacgt ccgtcaggaa gtaggcgagc agcaggccgg
     1441 ccgtgaccgc gatcatgccc aatgcgacgc tgccgaacag gttccgcgcc gcccggccga
     1501 tcaacccctc cttgatcagc gccatcgact cccacgtcca cgtcgagaag gtcgtctcgg
     1561 cgccgaggaa gccggtcgcg agcaacaatc gcatctgcgg atcgtcatcg aaccagccgg
     1621 cgagcagccc cagcaccagc ccgatcagga aggagccgcc gatgttggcg atcatcgtgc
     1681 cgaccgggaa gccggtcccg aaccgtcgcg ccgaccatac cgagatcccg taacgcacgt
     1741 tcgcgccgac gaatccaccc gcgctgacca gcaggaaggc gctcaccggt tccgctccgg
     1801 ttcagtcatc gaggccaact cagaattccg ctcgttgccg tgcgccatgc gcgaaagtgt
     1861 cgcatctggc cgccgctccg cactacattg gccatgtcat cgtctctttc gccgggaagc
     1921 gatcgcgagg tcagcatgga tcaccaactc gacgggcgcg gggtacgggt gcgggcctac
     1981 ttcggcgagc gggatcacca ccacggcaag ccgctctgga gcgcgctgct ggagttcctg
     2041 cgcgcgcacg gtgcggccgg cgcgaccgtc acccgcggga tcgccggcta tggcgcgcac
     2101 agcaagatcc atgctgcctc gatcgtcgat ctctcgtccg atctgccgtt ggttctcgag
     2161 tggatcgaca cggaagagcg ggccggaagc ctgctgccgg agctcgaaga gatgctgcag
     2221 ggcggcctga tcacgaccga tccggtcacc atcattcgct accagcccca caccgatcgc
     2281 gcttgacact gtcccatccc gcataatgtg cgccgtattg ccatcgagcc catgcgccgc
     2341 gttcgtcgcg cgctgagtcc tggagtgaag ttctgtgacg gccgtcgcta cccatcgtca
     2401 tgacgtcatc atcgtcggag ccggcgtggc cggctgctcg gccgcctatc gcctggccca
     2461 ggccggccgc aacgtgctgg tcctcgagaa gcggggcatc gcctccggcg cctccggccg
     2521 gaacggcggt atgaccggcg tcggctcagc gatgcatgcc gacgccggac cggccgtcta
     2581 ctcgattacc tcagccaacc tggccctcct caagcagctc ccggacgagc tggagaccga
     2641 cttcgagttg cgcctgcccg ggaccctcaa tgtggccatg accgaggacc attgggcata
     2701 tctcaatggc agcgtcgaag agcagcgcgc gggcggactc gatgtgacca tgatcgatgg
     2761 ctacgaggcg cgccagatga tgcccgccct gaccgagaag gcgatcggcg
//
LOCUS       DATAOZ010000217         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3247365, whole genome shotgun sequence.
ACCESSION   DATAOZ010000217 DATAOZ010000000
VERSION     DATAOZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3247365"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..661
                     /locus_tag="VHR64_05760"
     CDS             89..661
                     /locus_tag="VHR64_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3302369.1"
                     /translation="MATAALETTDTRTRLIEAAVDLFTRHSFAGTSLQMIADELGFTK
                     AAIYHHFRTREQLLAAVLEPILDELRTIVDSAETRRTAHSRAEHMLSGYAALTVRNRG
                     LVAVLAADPSVAAVLIERAEWGDLIRRQLALLADVDPGPAGEVKAAMVFAGMAGAAGP
                     TWGTFDDAALQEHLVDAGRRTLGLRAPRRS"
     gene            710..1411
                     /locus_tag="VHR64_05765"
     CDS             710..1411
                     /locus_tag="VHR64_05765"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3302370.1"
                     /translation="MKTAVVTGGGSGIGRAVAERLRADGYHVATIDLTASDEEFAQTA
                     DVTDRCQVDQALAAIRAQLGPVTILVNAAGLDGFKRFTDITFDEWRKVVDVNLNGVFH
                     CIQAVLPDMIDAGWGRIVNISSSSTHSGTAYMSHYVAAKSAVNGLTKSLALEYGPAGV
                     TVNAVPPGFIDTPMLRNAESRGRLGNVQATIDATPVRRMGKPEDIAAACAFLISDEAG
                     YITGQILGVNGGRNT"
     gene            1441..>1712
                     /locus_tag="VHR64_05770"
     CDS             1441..>1712
                     /locus_tag="VHR64_05770"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX3302371.1"
                     /translation="MSNGPAHFTLAEGGAYVPTPFAQSHWGDDHLNGPAVVGLAAREL
                     ENNCGSSEFMPTRFTADLFRAARNVPTTIDVRVVRDGRRVRSAECDV"
BASE COUNT          334 a          510 c          584 g          284 t
ORIGIN      
        1 tggagcctag tcgatcgtaa aggagctagc tttacgaatg gacggtacag taccatacga
       61 tcgtaaagcc gaacctgagg agctggcgat ggcgacagcg gcgttggaga ccaccgacac
      121 ccggacgcga ctgatcgagg ccgcagtcga cctgttcacc cgacacagct tcgcgggcac
      181 ctcgctgcag atgatcgccg atgaactcgg attcacgaaa gctgctatct accaccactt
      241 tcgaacccgc gagcaactgc tggccgctgt ccttgaaccg atcctcgacg agttgaggac
      301 catcgtggac tctgccgaga cgaggcgtac cgcgcactca cgggcagagc acatgctgag
      361 cggctacgcg gcgctgacgg tgcgcaacag gggactggtt gcggtgctag cggcggaccc
      421 gagcgtcgcg gcagtgctca tcgagcgcgc cgaatggggc gatctgatca ggcgacagct
      481 ggccctgctg gccgatgtcg acccgggacc cgccggcgaa gtgaaggcgg ccatggtctt
      541 cgccggaatg gcaggcgcgg cggggccgac atggggcact ttcgatgacg cggcgttgca
      601 agagcatctg gtggatgcgg gccgtcgcac gctgggcctg cgggcgccac gacgcagttg
      661 acatcaacta ccaagatatt caagcgattt cgggaaggaa gagacatcga tgaagacggc
      721 tgtcgtgaca ggaggcggct cgggtatcgg ccgggcggtg gcggaacggc tgcgggccga
      781 cggctaccac gtggccacga tcgacctgac cgcctccgat gaggagttcg cgcagaccgc
      841 agacgtgacc gatcgctgcc aggtcgatca ggcgttggcg gcgatccgcg cgcaactcgg
      901 gccggtcacg atcctggtca acgcggcagg cctggacggc ttcaagcgct tcaccgacat
      961 cacgttcgat gagtggcgca aggtggtgga cgtcaatctc aacggggtgt tccactgcat
     1021 ccaggcggtc ctgcccgaca tgatcgacgc gggctggggg cggatcgtca acatctcgtc
     1081 gtcgagcacc cattccggca ctgcgtatat gtcgcactac gtcgcagcga agtcggcggt
     1141 caacgggttg accaagtcgc tggccctgga gtacggaccg gcgggcgtca cggtgaatgc
     1201 cgtgccgccg ggcttcatcg atacgccgat gctgcgcaat gcggaatctc gcggcaggct
     1261 cggcaatgtc caagcgacca tcgatgcaac gccagtccgc cggatgggca aacccgaaga
     1321 catcgctgcc gcatgcgctt tcctgatctc tgatgaggcc ggttacatca cagggcagat
     1381 tctgggcgtc aacggcgggc gcaacacgta gccgatcagg cgctatgcga cgatgttcgg
     1441 atgtcgaacg ggccggcgca cttcacattg gccgagggcg gcgcgtacgt gccaacgccg
     1501 ttcgcgcaga gtcattgggg cgacgatcat ctgaatgggc cagcagtcgt cgggttggcg
     1561 gctcgcgaac tcgagaataa ttgcggatca tcggaattca tgccgacacg attcaccgca
     1621 gacttgttcc gtgcggcacg caatgtgccg acgacgatcg acgtgcgagt ggtccgcgac
     1681 ggaaggcggg tgcgcagcgc ggaatgcgat gt
//
LOCUS       DATAOZ010000218         6940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2459504, whole genome shotgun sequence.
ACCESSION   DATAOZ010000218 DATAOZ010000000
VERSION     DATAOZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2459504"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VHR64_05775"
     CDS             complement(<1..340)
                     /locus_tag="VHR64_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302372.1"
                     /translation="MSSTPLPAPSILALDERTVVTPTVRNRSRPIVTALRYLLLTIGA
                     LTMIVPFLWMVSTGLKAPGSVLTIPPELFPKEPTLASFRKVMDVVPLGRMFINSLFVT
                     VVVVTAQIVTG"
     gene            complement(333..1214)
                     /locus_tag="VHR64_05780"
     CDS             complement(333..1214)
                     /locus_tag="VHR64_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3302373.1"
                     /translation="MVGDTGWRRNGWIALFILPSMIGMLLFVIGPIIASAVLTLFEWD
                     LLTDPEFIGLGNFRRLIHDQQIRDALGHTLAFIIGYVPSVMILGLLLALILNGRLKGL
                     ALFRTAFFLPVVSSWVAVALLWKWLFNPRYGLINYGLEQIGITGPKWLFDPTWAMPAI
                     VITSVWKDLGFVAVLFLAGLQAIPDDVYEASSLDGAGKFEQLRSVTIPLLKPTIFFIS
                     IISIINSFQVFPQVWIMTEGGPAGSTTVIVERVVKHAFSYGEMGYAATISWVLFVLVF
                     GVTAFQLWLQRRGSLDV"
     gene            complement(1242..2450)
                     /locus_tag="VHR64_05785"
     CDS             complement(1242..2450)
                     /locus_tag="VHR64_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX3302374.1"
                     /translation="MAPRLQSANRQLIRQINESVVLGIIHDHGPISRTEIADIANLSP
                     ATISGIMHTLIRRGLVREQESGPSTGGRPRVLVEFARDAGCVIGIKLTETQLIGALTD
                     FGTETIAQRTIPLGPDRAPGAVVEQLDRLVKLLRSEAPERRFLGVGIGLAGAIDRRAG
                     ICRFSPYLPWRDVPLASLLEQRVGKPVVIENDVSALILAERRLGGADLTDFIVVTLGR
                     GVGMGMVLDGHPYRGGYGAGGEFGHITMDPHGPLCDCGKYGCLEALIGEPALTRRISE
                     VCGRETSLAEGAELARAGDKSLRSIFASAGETLGLALAAVINVLNPTRVTVGGEGIDV
                     LDLLLEPMRAALTAHCFDGLFDDIDLIVAPWGDDAWARGAAGLVLDELFHARLDIDLE
                     APIAGITNQA"
     gene            2803..3534
                     /locus_tag="VHR64_05790"
     CDS             2803..3534
                     /locus_tag="VHR64_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3302375.1"
                     /translation="MLKRRVLLVEDHPMYRNGVKRLLEDTGRFQVVGEAVNGHDAIHQ
                     ADIHRPEIVLIDIQLPGVTGLKIARVLRKQHHNMKIVFLSMHLDDERLFDAIRSGAVA
                     FLTKDIDQETLVDSLRRVAQGENLINQLILSRPQLAWKVLSEFRQLTGDNEEAREREI
                     AFAALPLSAREIEVLDCVAQGFSNKEIADELYVTEQTVKNHMTSVLRKLDVNDRVQAV
                     LYAVKNGWIEIGPQPYAQVEMARSA"
     gene            3613..4308
                     /locus_tag="VHR64_05795"
     CDS             3613..4308
                     /locus_tag="VHR64_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302376.1"
                     /translation="MMNALLIGTDRWRDEHSGSSAAVVRYRGLSAGDENETLAAMKRG
                     LEAELRSRWGSAARQELLADPTLAAYQRHDQRFGQNYHVAMQIMSIAQKGKPIPNRNP
                     LIEVMFMTELKTGVLASAQDADQIAFPIAIDSADGSEQYTRYDGVEERCKAGDQLMRD
                     ADGEILTSIAQGPTAFGLITLETSSVAYCFYYPSDVPELAITESLAYLDQCIHAACPN
                     AELSGKTVIAACM"
     gene            complement(4313..5602)
                     /gene="purD"
                     /locus_tag="VHR64_05800"
     CDS             complement(4313..5602)
                     /gene="purD"
                     /locus_tag="VHR64_05800"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX3302377.1"
                     /translation="MSMRILVIGGGGREHALCWRLAHSQSTQVLFCAPGNPGIGRYAE
                     NVPIRATDIDELTHFAVDREVDLVVVGPEAPLALGLADRLRRRGIAVFGPSQDAARIE
                     TSKQWAKSLMLDAGVPTARYVAVREFSQALIALQQFDYPVVLKADGLAAGKGVVTVGS
                     YQEAVWALTSMLEEHALGPAAETVLIEERLTGPEVSILALTDGDAIRILTPSCDHKRA
                     FDRDQGPNTGGMGAFAPTGLVDRELLERIDQTILRPTVDAMRARGCPMQGVLYAGLML
                     TEHGPMVLEFNCRFGDPETQVVLPMIEGDFAELCHAGATGTLGQTDLHLVPDLAAVGI
                     VVASAGYPGPYRTGLPIAGLDAAAEEAFVFHAGTDRNADGEIVTAGGRVLTVVGLGSS
                     IPEAQLHAYASAEQISFEGAFYRHDIGAREHAHARIR"
     gene            5645..6772
                     /gene="rlmN"
                     /locus_tag="VHR64_05805"
     CDS             5645..6772
                     /gene="rlmN"
                     /locus_tag="VHR64_05805"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX3302378.1"
                     /translation="METATERPRSTPPTIHLRRAKATSVYDLTLDELTEWMTERGYPA
                     YRARQLFDALYRQLASSYDEVSVLPKALRAELADALPIAAMVPVNEVSTHNRDTVKTL
                     YRTQLGDLVETVLMLYRSRATVCVSCQVGCAVGCAFCATGLMGLDRNLTTGQMVAQVI
                     DAARTARDMGRELSNLVMMGMGEPFHNYPATIKFLTLLNDLNGFGMGARRMTVSTSGV
                     VPFIDKLAEEPLQVNLAISIHAPNDELRSQLVPINRKHPIADLMAAVDRYADKTHRRV
                     SFEYALMSGINDSDEVALDMAKLMRGRLCHLNVIPFNKVDVLEFERPTADGIERFAEL
                     AGSLGTPVTVRYSRGLDISAACGQLRARQLQKSEEPVEHSI"
BASE COUNT         1332 a         2214 c         2237 g         1157 t
ORIGIN      
        1 atccggtcac gatctgcgcc gtgaccacga ccaccgtcac gaagaggctg ttgatgaaca
       61 tccggccgag cggaacgacg tccatcactt tgcggaagct ggcgagggtc ggctccttcg
      121 gaaagagctc gggcgggatg gtgaggacgc tgccgggggc cttcaacccg gtcgagacca
      181 tccagaggaa gggcacgatc atcgtcaggg cgccgatagt cagcagcaag tagcgcaggg
      241 cggtcacgat cggtcgcgat cgattccgga ccgttggcgt cacgaccgtg cgctcatcga
      301 gggcgaggat ggaaggagcg ggcaagggtg tgctagacat cgagcgaccc cctccgctgc
      361 agccagagct ggaaggccgt gaccccgaag accagcacga agagcaccca cgagatcgtc
      421 gcggcgtagc ccatctcccc gtaggagaag gcgtgcttga ccacccgctc gacgatcacc
      481 gtggtcgacc cggcgggtcc accctcggtc ataatccaga cctgcggaaa gacctggaag
      541 gagttgataa tcgagatgat cgagatgaag aagatcgttg gcttgagcag cggaatcgtg
      601 actgagcgca actgctcgaa cttgcccgcg ccgtccaggg aggacgcctc gtagacatcg
      661 tccgggatcg cctgcaatcc cgccaggaag agaacggcca caaagccgag gtccttccag
      721 acactggtga tgacgatcgc gggcatggcc caggttgggt cgaacagcca cttcgggccg
      781 gtaatcccga tctgctccaa cccgtagttg atcagtccgt agcgcggatt gaagagccac
      841 ttccagagca gcgcgaccgc gacccacgat gagacgaccg gcaggaagaa ggcggtccgg
      901 aagagggcga gtcccttgag cctgccgttc aggatcaggg cgagcagcag accaaggatc
      961 atgaccgagg ggacgtagcc gatgatgaat gccagcgtgt gacccagcgc atcccggatc
     1021 tgctggtcgt gaatcagacg gcggaaattg ccgaggccga tgaactctgg atcggtcagc
     1081 agatcccact cgaaaagcgt cagcaccgcc gaggcgatga tcgggccgat cacgaagagg
     1141 agcatgccga tcatgctggg caggatgaag agcgcgatcc agccgtttcg cctccatccc
     1201 gtgtcaccaa ccatggtctc cctcctttcc gtcccgtgcg cctatgcctg gtttgtgatc
     1261 ccggcgattg gtgcttccag gtcgatgtcg aggcgcgcat ggaaaagctc gtccaggacc
     1321 agcccggccg cgccacgcgc ccaggcgtcg tcgccccatg gcgcgacgat caagtcgata
     1381 tcgtcgaaga gtccgtcgaa gcaatgggcg gtcagggcgg ctcgcatcgg ctcgagcagc
     1441 aggtcgagca cgtcgattcc ctcgccgccg acggtgaccc gggttgggtt gagcacgttg
     1501 atcacggctg ccagagccag accgagcgtc tcgcccgcgc tcgcgaagat gctgcggagc
     1561 gatttgtcac cggctcgggc cagctccgcc ccttcggcga gcgaagtctc ccgcccgcag
     1621 acctcggaga tccggcgggt gagggccggt tcgccgatca gcgcctcgag gcagccgtac
     1681 ttgccgcaat cgcaaagcgg gccgtgcgga tccatcgtga tatggccgaa ctcgccgccg
     1741 gcgccataac cgccgcgata cgggtgacca tcgaggacca tgcccatgcc aacgccgcgg
     1801 ccgagcgtga cgacgatgaa atcggtcaga tccgcaccac ccagccgacg ttcggccagg
     1861 atcaacgcgc tgacatcgtt ctcgatcacg accggctttc ccacccgctg ctcgagcagc
     1921 gaagcgagcg gaacatcgcg ccagggcagg tacggcgaga accggcagat tccggcgcgg
     1981 cgatcgatcg cgccggcaag gccgatgcca acgccgagga agcgacgttc cggcgcctcg
     2041 gagcgcagca gcttcacgag tcgatcgagc tgctcgacga ccgccccggg agcccggtcg
     2101 ggaccgagtg gaatcgtccg ctgggcaatt gtctcggtgc caaagtcggt cagcgcgccg
     2161 atgagctggg tttcagtcag cttgatgccg atcacgcagc cggcatcgcg ggcgaactcg
     2221 accaggacgc ggggacgtcc gccggtcgaa gggccagact cctgctcgcg gaccaatccg
     2281 cgacggatca gggtatgcat gatgccggag atcgtggccg gcgagaggtt ggcaatatcg
     2341 gcgatttcgg tccgggagat tgggccatgg tcgtggatga tgccgagcac gaccgattcg
     2401 ttgatctgcc ggatcagttg ccgattggcc gattgcagac ggggcgccat tgccattttc
     2461 ctgagcgaaa tcgaagccgt cgctttcttc attcactgaa taaagaaagc ctatggccgt
     2521 cgtcaggagt tgtcaagagc gtccgggaga gacaggaccg ggccagtagt gtcgacccga
     2581 tcctgtgaca gagtggcaca taggacctat cgccgttccg accttagtac tgttcgccag
     2641 tacccagggg ccaaaatgcg ctattccatt cgattctagg caaacctact cttgtccctg
     2701 gaagagcgag atgcctcgcc gccgaccccg aagccggttc ccgccggaac acgagatccc
     2761 actcgcagtt gtgcagcatt accttgggta ggggaaatcg acatgttgaa gcgacgcgtg
     2821 ctgttggttg aggatcaccc gatgtatcgc aacggggtca agcggctcct cgaggatacc
     2881 ggccggttcc aggtcgtcgg cgaggcggtc aatggtcacg atgcgatcca tcaggccgat
     2941 atccatcggc cggagattgt cctgatcgac attcagctcc ccggtgtgac cgggctcaag
     3001 atcgcccgcg tcctccgcaa gcagcaccac aacatgaaga tcgtcttcct ctcgatgcac
     3061 ctcgacgacg agcggctgtt cgacgcgatc cggtccggcg cggtggcctt cctgaccaag
     3121 gacatcgacc aggagacgct ggtcgactcg ctccgccgcg tcgcccaggg cgagaacctg
     3181 atcaaccagc tcatcctctc ccggccgcag cttgcctgga aggtgctctc cgagttccgg
     3241 cagctcaccg gcgacaacga agaagcgcgc gagcgcgaga tcgccttcgc cgcgctgccg
     3301 ctctcggccc gggagatcga ggtgctcgat tgcgtggcgc aggggttctc gaacaaggag
     3361 atcgccgacg agctttacgt taccgagcag accgtcaaga accacatgac ctcggttctc
     3421 cgcaagctgg acgtcaacga tcgcgtccag gcggtgctct acgcagtcaa gaacgggtgg
     3481 atcgagatcg gtccgcaacc atacgcgcaa gtcgagatgg cgcgctctgc ctagtcgcgc
     3541 cgaagttcaa ttgacgaaac gcgggcgcag ctggcgcgcc cgcgtttcgt ttgtcccggg
     3601 tacgcttccc gcatgatgaa cgcacttctg atcggaaccg accgctggcg agacgagcat
     3661 tccggctcaa gcgcggcagt cgtccggtac cgcggtctgt ccgcgggcga tgagaatgag
     3721 acgctggccg ccatgaagcg cgggcttgag gccgagctgc ggtcgcgctg gggttcagcg
     3781 gccaggcagg agctgctggc tgatccgacg ctggctgcct accagcgcca tgatcagcga
     3841 ttcgggcaga actaccacgt ggccatgcag atcatgtcga tcgcacagaa gggcaagccg
     3901 atcccgaatc ggaacccgct gatcgaggtc atgttcatga ccgagctgaa gaccggcgtg
     3961 ctggcctcgg cccaggatgc ggaccagatc gcgttcccga tcgcaatcga cagcgccgac
     4021 ggctccgagc agtacacccg gtacgacggc gtcgaggagc ggtgcaaggc cggcgatcag
     4081 ctcatgcgcg acgcggacgg cgagatcctg accagcatcg cgcaaggccc gaccgcattc
     4141 ggcctcatca ccctggaaac gagctcggtc gcctactgct tctactaccc cagcgacgtc
     4201 ccagagttgg cgatcaccga atcgctcgcc tacctcgacc agtgcattca cgccgcctgc
     4261 ccgaacgcgg aactatccgg caagacagtg attgccgcat gcatgtaggg ttttagcgga
     4321 ttcgggcgtg ggcgtgttcg cgggctccga tgtcgtggcg gtagaaggcg ccttcgaagc
     4381 tgatttgctc ggcggaagcg taggcgtgga gctgagcttc ggggatcgag ctgcccagtc
     4441 cgacgacggt caggacgcgg ccaccagcag tcacgatctc gccgtcggca ttgcggtcgg
     4501 tgccggcgtg gaagacgaaa gcctcctcgg ccgcggcatc gagtcccgca attggcaagc
     4561 cggtgcggta tggacccgga tagccggctg aagccaccac gatcccgacg gcagccaggt
     4621 ccgggaccag gtggagatca gtctggccga gggtgccggt cgcgcccgcg tggcagagct
     4681 cggcgaagtc gccctcgatc atcggcagga caacctgcgt ctcgggatcg ccgaaacggc
     4741 aattgaactc gagcaccatc ggtccgtgct cggtcagcat caatcccgcg taaagcaccc
     4801 cctgcatcgg gcagccacgc gcgcgcatgg catccaccgt cggccgaagg atcgtctggt
     4861 cgatgcgctc cagcagctcc cgatcgacca gtccggtcgg ggcaaaggcg cccatcccgc
     4921 cggtattcgg accctggtcg cggtcgaagg cgcgcttgtg atcgcacgac ggcgtcagga
     4981 tgcggatcgc gtccccgtcg gtcagggcca ggatcgaaac ctcgggaccg gtcagacgct
     5041 cctcgatcag aaccgtttca gccgccgggc caagcgcatg ctcctcgagc atcgacgtca
     5101 acgcccagac cgcctcctgg tacgagccga ccgtcacgac gcccttgccg gctgccaaac
     5161 cgtctgcctt gagcaccacc gggtagtcga attgctggag cgcgatcagg gcctgcgaga
     5221 actcgcgcac tgccacgtat cgcgctgttg gaacgcccgc atccagcatg agcgacttgg
     5281 cccattgctt gctggtctcg atccgggccg cgtcttgcga cgggccgaat accgcgatcc
     5341 cccgccggcg cagacgatcg gccaggccga gtgcgagcgg cgcctcggga ccgaccacga
     5401 ccaggtcgac ctcgcggtcg acggcgaagt gcgtcagctc gtcgatgtcg gtcgcgcgga
     5461 tcgggacatt ctcggcgtat cgcccgatac ccggattgcc cggtgcgcag aagagaacct
     5521 gggttgattg gctatgggcg agtcgccagc agagcgcgtg ctctcgacct ccgccgccga
     5581 tgaccaggat acgcatgctc atggcccgag tgtactactc ggcgcatgct agaattcgtc
     5641 ggttatggaa actgccaccg agcggcctcg ctcgacgccc ccgacaattc acctgcgccg
     5701 cgcaaaggcg acttcggtct atgatctgac gctcgacgag ctgactgaat ggatgaccga
     5761 gcgcggctat cccgcgtacc gggcccggca gctcttcgac gcgctctaca ggcagcttgc
     5821 ctcgtcatac gacgaggtct ccgtgctgcc caaggcgctg cgggcggagc tggccgatgc
     5881 cctgccgatc gcggcgatgg tcccggtcaa cgaggtttcg acccacaacc gcgacacggt
     5941 caagacgctc taccggacgc agctcggcga tctggtcgag accgtgctga tgctctaccg
     6001 atcgcgcgcg accgtctgcg tatcgtgcca ggtcggctgc gcggtcgggt gcgcattttg
     6061 cgcgaccgga ttgatggggc tggaccgcaa cctgacgacc ggccagatgg tggcgcaggt
     6121 gatcgacgcg gcccggacgg cccgtgacat ggggcgagag ctgagcaacc tggtcatgat
     6181 ggggatgggt gagcccttcc acaactaccc ggccacaatc aagtttctga cgctgctcaa
     6241 cgacctaaac ggattcggca tgggcgcgcg gcggatgact gtctcgactt cgggcgtcgt
     6301 cccgttcatc gacaagctgg ctgaagagcc gctccaggtc aacctcgcga tctcgatcca
     6361 cgcgccgaac gatgagctcc gctcgcagtt ggtgccgatc aaccgcaagc acccaatcgc
     6421 cgacctgatg gcggcggtcg atcgatatgc ggacaagacg caccggaggg tctcgttcga
     6481 gtacgcgctg atgagcggga ttaacgacag cgacgaggtc gcgctcgata tggccaagct
     6541 gatgcgcggc cggctctgtc acctgaacgt gatcccgttc aacaaagtcg acgtgctcga
     6601 gttcgagcgg ccgactgcgg acggcattga gcgattcgca gagctggccg gctcgctggg
     6661 cacgccggtg accgtgcgct actcccgtgg cctggatatc tccgctgcct gcggccaatt
     6721 gcgggccagg caacttcaga aaagcgaaga gccagtcgag cattcaatct agcagcgaac
     6781 gcgtcgtcaa tgcgggtacc acccgaagtc gatttcgttg ccgtcgggat cctgaaacgt
     6841 gaagatgcgg acattcccgt cgttcgtccc ttgtttggtc ggttcgatcc cacgttccgc
     6901 gatggcggct atgaccgcat ccagatcgtc gaaatacatc
//
LOCUS       DATAOZ010000219         8064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1407694, whole genome shotgun sequence.
ACCESSION   DATAOZ010000219 DATAOZ010000000
VERSION     DATAOZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407694"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VHR64_05810"
     CDS             complement(<1..560)
                     /locus_tag="VHR64_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302379.1"
                     /translation="MSSTPVSDFGLVQDGSTLDNAPEPPKTGTLTISDEVTRPDGSHQ
                     LVLNMGPQHPSTHGVLRVILGLDGEVITDCDPVIGYLHRGVEKIGEQHRYAQVVTWTD
                     RTDYVAAPMNNLGCIMAIEKLCDVTAPERAQYWRVIMSELARIASHLVWLGTHALDIG
                     ALSMSLYCFRERELILDIFELFCGAR"
     gene            complement(566..1204)
                     /locus_tag="VHR64_05815"
     CDS             complement(566..1204)
                     /locus_tag="VHR64_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX3302380.1"
                     /translation="MSEGNLPQTVETDQTPVATEPVDPWVRENYFDPDVERHPAVRVI
                     RETFPGAILDIVRFRDETTIHIRPELLLEICALLRGHQQVGMDVLTDITAVDMLRLRQ
                     APRFDVVTQLYSLRNRVRLRLKSGIDEGQAVPSLVPLWNGANWLERETYDMFGIVFEG
                     HPNLRRILLADDWDEGFPLRKDYPLRGWKEFPVYNTERTVGRVRTRWTGRGV"
     gene            complement(1223..1774)
                     /locus_tag="VHR64_05820"
     CDS             complement(1223..1774)
                     /locus_tag="VHR64_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX3302381.1"
                     /translation="MGLIDDRFDKNVFTTSLDFLFNWGRRSSLWWLQFGLACCAIEMI
                     AASMARFDLAERFGMLYRASPRQADLMIVAGTVTKKMAPVVRQLYDQMPEPKWVISMG
                     SCANVGGPFDSYAVVQGVDQVVPVDIYVPGCPPTPEALYYGVLELQNKVIKYQTMAKK
                     QGAAAAEADRIASREEAMAAVRG"
     gene            complement(1765..2130)
                     /locus_tag="VHR64_05825"
     CDS             complement(1765..2130)
                     /locus_tag="VHR64_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX3302382.1"
                     /translation="MEVHGGNWAVIGLMVVAAFAMANTLLILNLIVAPSKPSKAKAAP
                     YESGVPDVSPVKPRYTPRFYVVAMLFVVFDIEAIFIYPWAVNFDQLGLFGMVEMFSFI
                     GLLGIGYVYAWKKGALEWV"
     gene            2327..2989
                     /locus_tag="VHR64_05830"
     CDS             2327..2989
                     /locus_tag="VHR64_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302383.1"
                     /translation="MSALRLALRTYRDLYDHIWIMLLVTAVWWVLLITVIFAPPATLL
                     LFRHADPRIGPWEDRPGLRESGEFLWAQLIRSWLITLATLPIIALTAFNLDYYGSGVS
                     ALSFLSPLWLVLLILAITATFMIFAVAGVTDLSPKQAIVAGARITGARLPAALVLLLF
                     TAIIPVMAIVASLYVLFPLAILIPALVAIAFSTFVLKATRAPLPEPNQPTEERLHEKR
                     SG"
     gene            complement(3003..5930)
                     /locus_tag="VHR64_05835"
     CDS             complement(3003..5930)
                     /locus_tag="VHR64_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3302384.1"
                     /translation="MANESRPQFASLLRRFRLAAGLTQEALAERAHLSARAISDLERG
                     LKRSPRRDTVELLETALSLSPRERAAFEMAARGFDPGVQGNGSNPDAQAEPLPSAGSG
                     VAGDADQPSLIGRDNELALLALHAQRGCPPLLLFAGEPGIGKSRLLHEAATVAANHGY
                     QVLVGGCIRRHGQEPFEPFLEAMDRYLRQLDPVQLRSALRGCAWLIRLLPELKDGPIE
                     SPVAASLAPAQERRLIFQAVAQFLRNVAGPNGIMLVLDDLQWIGPDALDLLQAMLRNG
                     SDLPIRVVGAYRDTELIPGDPLALALADLTRSGVVLRSPVEPLNAAESRSLLDSLPES
                     SSAGWPALRQRILNRAEGVPFYLVSCATALSRGNPENDVPWDVAQSIRQRVDLLTDPA
                     RTVVRSAAVVGRVADANLVVSASELSEEDAVAALDEAVQSRLIEWAGPLSLRFPHDLI
                     REVVEADLGPARRAFLHRRIARLVESAWDEQPEREDLPADLLAYHFEHGGEPEAAIDY
                     AIRAADFAHRAGAHREEVALIGRAIDLAEPIWLAEATAGLRIDRGRAYFDFGMWSEAR
                     ADLEAGLAGLPANDLDRQCRALVDLAWVCHWLFDVPATRRHASDALALARKLRRDDLI
                     AEAMSAAAFAESSEGELKSSLRKYRSALAITPARSKPRLVPAAEMRSLILYWTGSYED
                     AIRHGQEALIAAQEARDTTYTARVLGNLGLAFTGRGRYGDAIDAFGEARRFCREHELS
                     QWLARSVAMEGGLHLAMLDYAGAEDLANEARELGLSIAWPLAVVSADIDLLLNFARRG
                     DPGGRTDQLLCDAVEAAAAATGAHGWLWKLRLAEARAEIALARGDISLAGALASDAVA
                     QARVLGRAKYVAIGLATRASALERLGQVREAVIDLHEAVHVARSTADPSVLLQALLPL
                     NTIDGNDQLAAEIGAVAAQVARSLPGEDMRDRFLSGLSRFDGGERVSLEADAGESIGE
                     PLT"
     gene            6174..6734
                     /locus_tag="VHR64_05840"
     CDS             6174..6734
                     /locus_tag="VHR64_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302385.1"
                     /translation="MSIGMRNVRVHGVVITLTLAIGLIVGMSVGNGSTRAQGSMPDLP
                     QGATGVTAAVLGMIQPAAAPGYDLQLFRSEWAPGSSISLHTHPGALVSCIESGSVSFA
                     IQSGMASVIRAHGPQTPAAPETISPNEPITYGPGDCVTFDQDAANTAHIAWNDGTEPA
                     VIWEAHLYKTGVKASTFTNEEGTPAP"
     gene            6916..7236
                     /locus_tag="VHR64_05845"
     CDS             6916..7236
                     /locus_tag="VHR64_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302386.1"
                     /translation="MYASLSTWQLDEAIQDDATYHAFVRDVLRRTLPTMRDIGILDSM
                     IVRTAPDTIVSVTVYESEDAAKAAWVEASGPMRRLYEARLTFVSRLIGPADDMPQLTD
                     DGRA"
     gene            complement(7255..>8064)
                     /locus_tag="VHR64_05850"
     CDS             complement(7255..>8064)
                     /locus_tag="VHR64_05850"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3302387.1"
                     /translation="RVASVGVMGGFGDDLLWIDGVKLMPDGGAGDRTAKMYEPYEGEP
                     DNRGTWTVPPDELVGHIQWAHEHGYAMDIHTCGDEAQDVAVAAFGKAQETCPNPRLRH
                     RVHHAYLPTEQALTTMAKHQIPAVVSNPFLWSLGESYITSLGRDRASRMMPMRTYLNR
                     GVPLAGSSDSPVATHNPWIGIASTISRTTVQGTELGPDERLTPDEALQMYTTGGAFVI
                     HREATLGSIEPGRQADLIVLDYDPRERTPAELMGTEARATMVGGRWVHDAR"
BASE COUNT         1470 a         2640 c         2509 g         1445 t
ORIGIN      
        1 aaccgggcgc cgcagaacag ctcgaatatg tcgaggatca actcgcgctc gcggaagcag
       61 tacagcgaca tcgaaagcgc gccaatatcg agggcgtggg tgcccagcca aacgaggtgc
      121 gacgcgatcc gggccagctc cgacatgatc acgcgccagt actgggcgcg ctcgggcgcg
      181 gtcacgtcgc aaagcttctc gattgccatg atgcagccga gattattcat cggcgcggcg
      241 acatagtcgg tccggtccgt ccaggtcacg acctgggcgt agcggtgctg ctcgccgatc
      301 ttctcgaccc cccggtgaag gtagccgatg accgggtcgc agtcggtgat cacctcacca
      361 tccaggccga ggatgacccg gagcacgccg tgggtactcg gatgctgcgg acccatgttc
      421 aggacgagct ggtgcgagcc gtccggccgc gtcacctcat cactgatcgt cagcgtgccg
      481 gtcttgggtg gctcgggtgc gttgtcaaga gtcgacccgt cctggacgag cccgaaatcg
      541 gaaactggcg tgctgctcat ggtctctaca ctccccgccc ggtccagcgc gtgcggacac
      601 ggccgaccgt gcgctcggtg ttatagacag ggaactcctt ccagccacgc aacggatagt
      661 ccttgcgcag cgggaaaccc tcgtcccagt cgtccgccag gaggatccgc cgcaaattcg
      721 ggtgaccctc gaagacgatg ccgaacatgt cgtacgtttc gcgctccagc cagttcgcac
      781 cgttccagag cggaaccagc gatgggacag cctgcccttc atcgatgccg gacttgagcc
      841 ggagccggac gcggttgcga agcgagtaga gctgcgtcac gacgtcgaac cggggtgctt
      901 gccgcaggcg cagcatgtcg accgcggtga tgtcggtcag gacatccatg ccgacctgct
      961 gatgcccacg aaggagcgcg cagatctcca gcaggagctc gggccggatg tggatcgtgg
     1021 tctcgtcgcg aaatcggacg atgtcgagaa ttgcgcccgg gaaggtctcg cggatgacgc
     1081 gcacggcggg atgccgctcg acgtcggggt cgaagtagtt ttcgcgcacc cacggatcga
     1141 ctggttcggt agcgaccggc gtctggtccg tctccaccgt ctggggcagg ttgccctcgc
     1201 tcacgcgtta ctccctttgc gcttagccgc gaacggcagc catggcctct tcgcggctgg
     1261 cgatgcgatc ggcctctgcc gccgcggcac cctgcttctt ggccatggtc tgatacttga
     1321 tgaccttgtt ctggagctcg aggacgccgt agtagagcgc ctcaggcgtc ggcgggcagc
     1381 ccggcacata gatgtcgacc ggaacgacct ggtcgacgcc ctggaccacc gcgtacgagt
     1441 cgaatgggcc gccgacattc gcgcacgagc ccatcgagat cacccacttc ggctccggca
     1501 tctgatcgta gagctgacgg acgaccgggg ccattttctt ggtcacggtg ccggcgacaa
     1561 tcatcaggtc ggcctggcgc ggagaggcgc gatagagcat cccgaaccgc tcggcgaggt
     1621 cgaaccttgc catcgatgcg gcaatcatct cgatcgcgca gcaggccaga ccgaactgca
     1681 gccaccagag cgacgagcga cgcccccagt tgaagaggaa gtcaagcgac gtcgtgaaga
     1741 cgttcttgtc gaatctatcg tcgatcagac ccattccagg gcccctttct tccaggcata
     1801 gacgtagccg ataccgagca atccaatgaa ggagaacatc tccaccattc cgaacaggcc
     1861 caattggtcg aaattgaccg cccacgggta gatgaagatc gcctcgatat cgaataccac
     1921 gaagagcatt gccacgacgt agaagcgggg ggtgtatcga ggcttgaccg gactgacatc
     1981 cggcaccccg ctttcatatg gagccgcctt cgccttggac ggcttggacg gtgcaacgat
     2041 caggttgagg atcagcagcg tgttggccat cgcgaatgcc gctacgacca tcaggccaat
     2101 gactgcccag tttccgccgt ggacttccac gttgtggcta actccggttc cagctcaaag
     2161 ggtcgtcgcc gggcgcgctg accaccccaa atggtagcac tatccgcctg gagcttctct
     2221 catatttgtc acgccgtgcc gcgttcggag cgctccggag cggcggcaac cggacgaggt
     2281 gcggtagatt catctgaatt gaaccgatcc gtgaatggac tcccaggtga gcgcgcttcg
     2341 tttggccctg cgtacctatc gcgacctgta tgaccatatt tggatcatgc tgttggtcac
     2401 ggcagtctgg tgggtgctgc tgatcaccgt gatcttcgca ccgccggcca cgctcctcct
     2461 cttcaggcat gcggatccgc ggatcgggcc gtgggaggat cgaccgggct tgcgcgagag
     2521 cggcgagttc ctctgggcgc aattgatccg gagctggctg attaccctcg cgaccctgcc
     2581 aatcatcgcg ctgacggcct tcaacctcga ctactacggc agcggggtaa gcgcgctctc
     2641 gtttctgtcg ccgctctggt tggtgctgct gatccttgcc atcacggcca cgttcatgat
     2701 cttcgccgtc gccggggtga ccgacctctc gccgaagcaa gccatcgtcg ccggcgcgcg
     2761 gatcacgggc gcgcggctcc cggcggccct cgtgctgctc ctcttcacag cgatcattcc
     2821 ggtgatggct atcgtggcgt cgttatatgt gctcttccca ctggcgatcc tgattccggc
     2881 cctggtcgca atcgccttca gcacgttcgt gctcaaagcc acgcgcgctc cactccccga
     2941 gccgaaccaa ccaactgaag agcgtctcca cgaaaaacgc tccggctgac cttttgcccg
     3001 ggtcaggtca gtggttcccc gatcgattct ccggcgtccg cttccaggct gactcgctca
     3061 ccgccatcga atcgtgatag ccccgacagg aaccggtcgc gcatgtcctc gcccgggagt
     3121 gaccgggcga cttgcgccgc cacggccccg atctcggccg ccagttggtc gttcccgtcg
     3181 atcgtgttta gaggaagtaa cgcctgcagc aagaccgaag ggtcagcggt cgatcgtgcg
     3241 acgtggactg cctcgtggag atcgataaca gcttcgcgaa cctgtccaag ccgttccagc
     3301 gcgctggccc gcgtcgctag gccgatcgct acgtacttcg cgcggcccag aacgcgcgct
     3361 tgcgcgacgg catcgctggc aagtgcccct gcgagcgaga tatcgccgcg agccagggcg
     3421 atctcagccc tcgcctctgc caggcgaagt ttccagagcc aaccgtgggc gccagtagct
     3481 gcggccgctg cctccacggc atcgcacaag agctgatcag tgcgtccgcc ggggtcgccg
     3541 cgccgggcaa agttcagcaa gaggtcgata tcggcgctta ccacagccag cggccaggca
     3601 atcgacaacc cgagttcgcg cgcttcgttg gcaagatcct cggcacctgc gtagtccagc
     3661 atggcaaggt gcaggccgcc ttccatcgcg accgatcggg ccagccattg gctcagctca
     3721 tgctcgcgac aaaaccgccg cgcctccccg aaggcatcaa tcgcatcgcc gtacctgcca
     3781 cgaccggtga acgccaagcc aaggtttccc aggacacggg cggtgtatgt cgtatctctc
     3841 gcctcctggg cggcaatcag cgcctcctga ccatgccgaa tcgcgtcttc gtagctccca
     3901 gtccagtaga ggatcagcga gcgcatctcg gcggccggca ccagccgcgg cttcgaacgc
     3961 gccggggtga tggcgagcgc gctccggtac ttgcgcaggc tcgacttcag ttctccttcc
     4021 gagctttcgg cgaaggcggc ggcactcatt gcctcggcga tcaagtcgtc gcgccgaagc
     4081 ttcctggcca aggcgagcgc atccgacgcg tggcgacgcg tggcagggac atcgaagagc
     4141 cagtggcaga cccaggctag atcgaccagg gcacggcact gccgatcaag gtcattggcg
     4201 ggcaacccgg caagtccggc ttccagatcc gcgcgggcct cgctccacat accgaaatcg
     4261 aaatatgccc gccctcggtc aatgcggaga ccggcagtcg cctccgcgag ccagatcggc
     4321 tccgccaggt caatggcccg gccgatgagt gcaacttcct cccggtgagc gccagcccgg
     4381 tgcgcgaaat cagcggcacg gatcgcatag tcgattgctg cttctggctc accgccgtgc
     4441 tcgaagtgat aggcgagcaa atcggcgggc aggtcctccc gctccggctg ctcgtcccag
     4501 gcagattcga cgagccgcgc gatgcggcgg tgcaggaacg cgcgccgggc gggaccaaga
     4561 tcggcctcaa cgacttcgcg gatcaggtcg tgcggaaacc gaaggcttag agggcccgcc
     4621 cattcaatca ggcgactctg gacagcctca tcgagggccg cgacggcatc ctcctccgac
     4681 aactcgctgg cagacacaac aaggttcgca tccgcgaccc gccccaccac tgccgcgctc
     4741 cgcacgacgg ttcgggcggg atcggtcaac aggtccacac gctggcgaat actttgcgcg
     4801 acatcccacg gaacgtcgtt ttcaggattg ccgcggctca acgccgttgc gcaactaacg
     4861 aggtagaacg gcactccctc ggcccggttg agaatgcgtt ggcgcagcgc cggccagccg
     4921 gcgctggaac tctcgggaag cgagtcgagc agactgcggg actccgcagc attcaacggc
     4981 tcaacgggac tgcgcaggac tacgcctgag cgtgtcaaat cggccagcgc cagcgccagg
     5041 ggatcgcctg gaatgagttc ggtatcccgg tacgcgccga cgacccggat aggcaaatcg
     5101 ctcccattgc ggagcatcgc ctggagcaga tcgagcgcat cgggaccgat ccactgcaaa
     5161 tcatcgagga ccagcatgat gccattcggc ccggcgacgt ttcgaagaaa ctgagcgacc
     5221 gcctgaaaaa tcaggcggcg ctcctgtgcc ggcgccaaac tggcagccac gggagactcg
     5281 atcgggccgt ctttcagctc agggagcaat cgaatcagcc acgcgcagcc gcgaagtgcg
     5341 ctccgaagct ggaccggatc cagttgtctg agataccgat ccatggcctc gaggaacggc
     5401 tcaaacggct cctgcccatg acgacggatg catccaccga cgaggacttg atatccatgg
     5461 tttgcggcga ctgttgcagc ttcgtgaagc aggcgcgatt tgccaatgcc cggctctccg
     5521 gcaaacagca gcaaaggcgg gcatcccctt tgagcatgaa gagccagaag ggcaagctca
     5581 ttgtctcgtc caatgagcga cggctgatca gcatcgccgg ccacacccga gcccgcgctc
     5641 ggcaacggct ctgcctgggc atccgggttt gatccgttac cctggacacc tggatcgaac
     5701 ccgcgagcgg ccatttcgaa tgcggcacgc tcgcgcggcg acaacgacaa ggcggtctcc
     5761 agcagctcaa cggtatcccg gcggggagat cgcttcaggc cacgctccaa gtcgctgatt
     5821 gcccgggcac tcaagtgcgc gcgctcggcc agcgcttcct gggttaatcc ggcggcaaga
     5881 cgaaaccggc gcaagagtga cgcaaattgc ggacggcttt cgttcgccat tggcccggcc
     5941 ttgtcccgtc gttacgggga gaagtgcttg caattttatg tttcgttttg cacgttaatc
     6001 ataatcctag cactccccac aagtaccccc ggatcagggc gctgtcgctc gtgtggagga
     6061 tgtgtgccga aaatgtgagt gagtggcgcg ggacgtgtat ggtggccgcg caccggcgct
     6121 cctagagtcc tccccaggca cttgccaata cgtaatcgcg aggggagtta cccatgagca
     6181 tcgggatgcg caacgtacgc gtgcatggag tcgtgattac tctgacgctg gcaattgggt
     6241 tgattgtcgg catgagcgtc ggcaatggct cgacgcgagc tcagggatcg atgccggacc
     6301 tgccacaggg cgcgaccggc gtcacagcag cagttctcgg catgatccag ccggccgccg
     6361 cgcctggcta cgacctgcag ctcttccggt cggaatgggc gccgggatcg tcaatcagcc
     6421 tgcacacaca tcccggcgcg ctggtttcct gcattgagtc aggctcggtg agcttcgcga
     6481 ttcagtctgg catggcgtct gtgatccgcg cacacgggcc gcaaacgccg gccgccccgg
     6541 aaacaatctc gccgaacgag ccgatcacct atgggccggg cgactgcgtc acgttcgacc
     6601 aggatgcggc aaacaccgcc cacatcgcct ggaacgacgg gaccgagccg gccgtgatct
     6661 gggaagcaca tctctataag acgggtgtga aggccagtac gtttacgaac gaagaaggca
     6721 cccccgcacc gtaactcggg tgcttgactg acgaggtcat ccggggcgtc agcgattcac
     6781 gctgacgccc cggatgtttg ctgcgcaagt gcagatccgt tacgggtagt tgcgttgcat
     6841 tgctatgatc gaggcacaga tgatttctgc cgcaaaccga tgcgcttcgc ccggaatcgc
     6901 ggggaggtcg cgaagatgta tgcctcgctt agtacctggc aattggatga ggccatccag
     6961 gacgacgcta cctaccatgc cttcgtccgg gatgtgcttc ggagaacgct tccgaccatg
     7021 cgcgacatcg ggatcctcga ctccatgatc gtgcgcacgg cgccagacac gattgtctcg
     7081 gttacggtct acgaatcgga ggatgccgcc aaagccgcct gggttgaggc gagcggaccg
     7141 atgcggcggt tgtacgaggc caggttgacg tttgtcagcc ggcttatcgg tcccgcggac
     7201 gacatgcctc aacttaccga cgacggccgc gcctagcgcg aatggcggac ctggtcagcg
     7261 cgcgtcgtgc acccagcggc cgccgaccat cgttgcgcgc gcctccgttc ccatcaactc
     7321 ggccggcgtt cgctcgcgag ggtcgtagtc gagcacgatc aggtcggcct gcctacccgg
     7381 ctcgatcgag ccgagtgtcg cctcgcggtg gatcacgaac gcgccgcccg tggtgtacat
     7441 ctgcagcgcc tcgtccggcg tcaaccgctc gtccgggccg agttcggtcc cttgcacggt
     7501 cgtccggctg atcgtgctgg cgatgccgat ccatgggttg tgcgtcgcca cgggtgaatc
     7561 ggacgatccg gccagtggca caccccggtt cagataggtg cgcatcggca tcatgcggct
     7621 ggcccgatcc cgcccaagcg atgttatgta gctctctccc aatgaccaca ggaacggatt
     7681 cgagacgacc gccgggatct ggtgcttcgc catcgtggtc agcgcctgct cagtcggcag
     7741 gtaggcatgg tgcacccggt gccgcaggcg cggattgggg cacgtctcct gtgccttgcc
     7801 gaatgcggca accgccacgt cctgcgcctc gtcgccgcag gtgtggatgt ccattgcgta
     7861 gccgtgctcg tgtgcccact gaatatggcc gaccaactca tccggcggaa ccgtccaggt
     7921 gccgcggttg tccggctcgc cttcgtaggg ctcgtacatc ttcgcggtgc gatcgccggc
     7981 gccgccgtcc ggcatcagct tgactccgtc gatccagagc aggtcgtcgc caaacccacc
     8041 catcaccccg acgctcgcca ctcg
//
LOCUS       DATAOZ010000220         5337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3248559, whole genome shotgun sequence.
ACCESSION   DATAOZ010000220 DATAOZ010000000
VERSION     DATAOZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3248559"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VHR64_05855"
     CDS             <1..377
                     /locus_tag="VHR64_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302388.1"
                     /translation="VLTIATGNAEGWTVSSLNGSPAGFDRPSDIAVFTCPFEVNARIV
                     LANDGVAHMAEDLGALLNTYRPEVGCLNAHHVLNLAVQRDSGRPRSDMAVAAIGRSAT
                     VEGPRILDARISIPVRRFGSDL"
     gene            374..757
                     /locus_tag="VHR64_05860"
     CDS             374..757
                     /locus_tag="VHR64_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302389.1"
                     /translation="MTRTPASIGRISVVGPCASGKSTVVRALRMHGYDAHVTSQEHSA
                     VSNLWNRQQPSVLIALQADLESVRLRRRNPRWSRTVWQAQQDRLADAFAHADYVADTS
                     NLNANQVVREVLDFLESRTPVDESE"
     gene            764..2383
                     /locus_tag="VHR64_05865"
     CDS             764..2383
                     /locus_tag="VHR64_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3302390.1"
                     /translation="MTPPRLFPVGSRIWRLLLLAVLISGLAVSGHAEQTRSADNGNEL
                     RLAGPVQGPETLDPAQMRDLSAVTILRQIYRGLIYYDDDLNPVPELAESYDISADGLI
                     YRFVLRPGSTFQDGSPITANDVAFSLSRAVNPDTVEGDTSLLAGPTFLSDIVGFPDVI
                     NGQADMLSGIEVKSETELSITLTAPSATFLTRLAAVPAAIVSRAQVESDSTWWARPIG
                     SGPYRVDKWDPENELDLTRYDDFVLGKPKIKDINIRLGSRAIQSFNLYQADQIDVDAV
                     TLYDVDRVADPNGDFTDQLVRIPQFAFGYIALRTDVAPLDDPKIREALQLAFPRVNMA
                     TITFNGQVDPAFGLIPDGMLGRDWKKILPDMDIDAAKAAIQESSYGTADKVPPIRIYT
                     SGALASEVLRDIARDELGLKIEVFDLEWYDFLNRLDSKQVPAFELYWAADYPDPEAML
                     QPLWGTGWPGNYTDYSNPKVDDLLTQAASEADSDARAQLYAKAEQLILADNVVIPAYI
                     DVQYTVIKPYVHDLTVTPIGILRLEVATIDQ"
     gene            2431..3276
                     /locus_tag="VHR64_05870"
     CDS             2431..3276
                     /locus_tag="VHR64_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3302391.1"
                     /translation="MQPSDVDRLRLGWWSRLGAEEVRRLLAKAPGRSLWIPDTHEYIL
                     VGPWRHRTDVMHVQDLVAVRHPVELMRAAIMQAQEGGSRLFLAVEMAERRQDSFYDRV
                     GLNLLENVISYELPARPSLQESRAGIDIRRVDLLTDQELDSLVSVDWKSFPWLWQNSI
                     DEFREYYTQTGVELFVMREDGRPIGYLGTTVFPGWGHIDRLAVVRSEQGKGFGRLLTE
                     FAINHLVSLGAIRIGLSTQQRNERSQALYSRLGFRRQVAGDYRIYGRALWQNDAVDDL
                     VMGGA"
     gene            3277..4353
                     /gene="aroC"
                     /locus_tag="VHR64_05875"
     CDS             3277..4353
                     /gene="aroC"
                     /locus_tag="VHR64_05875"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX3302392.1"
                     /translation="MSSSFGKIFRLTTWGESHGPALGVVIEGCPAGIPLTVEEIQFQL
                     DRRRTGQSKVTTPRGEKDAAEILSGVFEGVTTGAPISIITYNKDADSSKYENLRNVFR
                     PGHADYTYWAKYGHRDHRGGGRSSARETWGRVAAGAVARKVLAHYGCEVYGFVREIGG
                     IEMHTFDRDQIEQNIVRCPDPIAAEKMINAILEAKEDKDSLGGIIEIRAVNPPVGLGE
                     PTFDKLDGLIGQAMLSIPAVKAVEIGEGFAVTRSRGSQANDWFQVVDGHVRARTNNAG
                     GTLGGISTGEEIVVRIAVKPTSSVARVQQTVTTDLEPTEIIVEGRHDPSVAPRAVPVA
                     EAMLANLLADLVLRNRAARIDFCD"
     gene            complement(4350..5135)
                     /locus_tag="VHR64_05880"
     CDS             complement(4350..5135)
                     /locus_tag="VHR64_05880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3302393.1"
                     /translation="MSQSLPSAHPYTEIAALYDLEHNRFRDDLDLIHYIVETVGDPVL
                     ELGCGTGRVLAHLADLEMRLTGIDNSPSMLERARARLDGVADVRLVESEMSTTGLPDE
                     TFGVVLLALNTLMHASTLDEQRRVLAEAFRVLDPRGQLFIDLPNPLSGAIDFVDHQVV
                     LEGNWDNEPAGRPVSKFSSRVIQRAEQVIQTNVWYDISGEVGQLGRIHTRFDLRYVYP
                     VELLLMLELAGFVEPKLYGTYELDPFSDSSPRLIATAEKTHSS"
BASE COUNT         1057 a         1566 c         1664 g         1050 t
ORIGIN      
        1 gagtgctgac gattgcgact ggtaacgcgg aaggctggac cgtgagctcg ctcaatgggt
       61 cgccggctgg ttttgatcga ccctcggata tcgctgtttt cacgtgcccg tttgaggtga
      121 acgcccggat agttctggct aacgacgggg tcgcgcacat ggcagaggat ttaggcgctc
      181 tgctgaacac gtatcgacca gaggtcggat gtctcaatgc ccatcatgtt ttgaatctgg
      241 ccgttcagcg agactccgga cgacctcgat cggacatggc ggtagcagca atcggccgct
      301 cggcgacggt cgaaggaccg cgcattcttg acgcccggat ctcgatcccg gtccgacggt
      361 ttgggagcga cctgtgaccc gtacccccgc gtcgatcggc aggatctcgg tcgtggggcc
      421 gtgcgcctca ggcaagtcga ccgtggtccg tgccttgcga atgcatggct acgacgccca
      481 tgtcacgtca caggagcaca gcgcagtctc caacctctgg aatcggcagc aaccatcggt
      541 gctgatcgcg ttgcaggccg atctcgaatc ggtacgtctc cgccgccgaa acccgcgttg
      601 gagccggacg gtctggcagg cacagcagga tcgcctggcc gacgcatttg cccatgcgga
      661 ctacgtcgcg gacacctcga acctgaatgc caaccaggta gtccgggaag tgctcgattt
      721 tctggagtca cgcacaccag ttgatgaaag cgagtaaatc gtcgtgactc caccacgtct
      781 attcccggtc ggatcccgga tttggcgact cctgctcctg gcggtgctga tttccggtct
      841 cgcggtcagc gggcatgcgg aacagaccag atcagcagac aacggcaatg agcttcgcct
      901 ggccggcccc gtgcagggac cagagaccct ggacccggcc cagatgcgcg acctctccgc
      961 ggtcacgatc cttcggcaga tctatcgcgg actgatctac tacgacgatg accttaatcc
     1021 cgtacctgag cttgccgagt cgtatgacat ttcggctgat gggttgatct acagattcgt
     1081 tctccgcccg ggatcgacct tccaggacgg cagcccgatc acggccaacg acgtggcgtt
     1141 ctcgctgtcg cgtgccgtaa atccggacac ggtggagggc gacacatcgc tcctcgccgg
     1201 gccgacattc ctgtctgaca tcgtcgggtt cccggatgtg ataaacggcc aggcagatat
     1261 gctcagcggc atcgaagtca aatctgagac cgagctctcg atcacattga cggcgccgag
     1321 cgcgaccttt ctgacccgcc tcgcagcggt cccggcagca atcgtaagtc gtgcacaagt
     1381 ggagtccgac tcgacctggt gggcgcggcc gatcgggtcc ggtccctaca gggtcgacaa
     1441 atgggatccg gaaaacgagc tcgacctgac ccggtacgac gacttcgtcc tcggtaaacc
     1501 gaagatcaag gacatcaaca tccgcctcgg ctcccgggcg atccagtcgt tcaatctcta
     1561 tcaggcggat cagatcgacg tggatgccgt caccctctac gacgtcgacc gggtcgcgga
     1621 tcccaatggc gatttcaccg accagctcgt gcgcatcccg cagtttgcgt tcggctatat
     1681 cgcgctccgg accgatgtcg cgccgctgga cgatccgaaa atccgggagg cgcttcagct
     1741 cgcgtttcca cgggtcaata tggcgaccat tacgttcaac ggacaggtgg atcctgcatt
     1801 cgggctgatc cctgacggaa tgctggggcg cgattggaag aagatcctgc ctgatatgga
     1861 tatcgacgct gccaaggcgg cgatccagga atcctcgtat ggaacggccg acaaggtgcc
     1921 gccaatcagg atttacacct ccggagcgct tgcgtcggag gtccttcgcg acatcgcgcg
     1981 ggatgagctc ggcctgaaaa ttgaggtttt cgatctcgag tggtacgact tcctcaaccg
     2041 gcttgactcc aaacaggtgc ccgcgttcga gctctactgg gcagctgact atccggatcc
     2101 cgaagcgatg ttgcagccac tctggggaac aggttggccg ggcaattaca cggactattc
     2161 gaatccaaag gttgacgatt tgctgacaca ggctgcttcg gaagccgatt cagatgcgcg
     2221 cgcgcagctc tatgccaaag ccgagcaact gatcctcgcc gacaatgtcg tcattccggc
     2281 atatatcgat gtgcaataca cagtcatcaa gccatatgta cacgacttga ctgtcactcc
     2341 gatcgggatc ttgcgactcg aagtggccac gatcgaccaa tgacgtcgca gccttcggag
     2401 cccgctgagc caccctatgg agctttgccc ttgcagccaa gtgacgtgga tcggctgcgg
     2461 ctcgggtggt ggtcccgact gggagcagaa gaggtgcgac ggctcctggc aaaagcgccc
     2521 ggccgctcgc tctggattcc tgatacccac gaatacatcc tggttggtcc gtggcggcat
     2581 cgaaccgatg tcatgcacgt ccaggatctg gtggctgttc ggcacccggt tgagctgatg
     2641 cgggcggcga tcatgcaggc acaggagggc ggcagccggc tcttcctcgc cgtcgagatg
     2701 gcggagcggc ggcaagactc attctacgac cgggttggac tgaatctcct cgaaaacgtt
     2761 atctcgtacg agctgcccgc gcgcccgagc ctgcaggagt ctcgcgcggg tatcgacatt
     2821 cgacgggtgg acttgctgac ggatcaggaa cttgactccc tggtcagcgt ggactggaag
     2881 tctttcccgt ggctctggca gaactcgatc gatgagttcc gggagtacta cacccaaacc
     2941 ggcgtggagc tcttcgtgat gcgtgaggat gggcggccaa tcggctacct cggcaccact
     3001 gtctttccag gctggggaca catcgatcgt ttggcggtcg ttcggagcga gcagggtaag
     3061 ggttttggcc gtctcctcac agagttcgca attaaccacc tggtctcact cggagcgatt
     3121 cgcatcggtc tgagtaccca gcaacggaac gagcgctctc aggcgcttta ctcgcgactc
     3181 ggtttccggc gtcaggtcgc gggcgattac aggatttacg gccgggcact ttggcagaat
     3241 gacgcagttg acgatctcgt gatgggcgga gcctgaatga gcagcagttt cggcaagatt
     3301 ttccgattga cgacgtgggg cgaatcgcat ggtcccgcgc ttggtgtcgt aatcgagggg
     3361 tgcccggccg gcatcccact gacggtcgag gagatccaat tccagctcga ccgtcgccgc
     3421 actggtcaga gcaaggtcac cactcctcgg ggtgagaagg atgcggcgga gatcctctcc
     3481 ggcgttttcg aaggagtcac gaccggcgcg ccgatctcga tcattaccta caacaaggac
     3541 gccgattcct cgaagtacga gaacctgcgg aacgtgttcc gacccggcca cgctgattac
     3601 acctattggg ccaagtacgg ccaccgcgat catcgcggcg gtggccgctc gtctgcgcgg
     3661 gagacgtggg gtcgggttgc ggccggcgca gttgctcgca aggtactggc gcattacgga
     3721 tgcgaggtct acggcttcgt gcgtgagatt ggtggcatcg aaatgcatac gttcgatcgt
     3781 gaccagatcg aacagaacat tgttcgctgc ccggatccaa ttgccgccga gaagatgatc
     3841 aacgccatcc tcgaggcgaa ggaagacaag gacagcctgg gcggcatcat cgagattcgc
     3901 gcggttaatc caccggtcgg gctcggcgaa ccgaccttcg acaagctgga tgggttgatc
     3961 ggtcaggcga tgctcagtat cccggcagta aaggcagtcg aaatcggcga ggggttcgcg
     4021 gttacccgtt cgcgcggatc gcaggcaaat gactggttcc aggtcgtcga tggccacgtt
     4081 cgtgctcgca caaacaatgc tgggggcact cttggcggga tctcgaccgg tgaggagatc
     4141 gtcgtccgga ttgcggtcaa gccgaccagc tcggtcgcac gagtccagca aacggtaacg
     4201 accgacctgg aaccgactga aatcatcgtc gaagggcggc acgatccgtc ggtcgcgccg
     4261 agggcggtgc cggtcgcgga ggcaatgctg gcgaacctgc tggctgatct ggtcctgcgg
     4321 aaccgggcgg cgcggatcga cttctgcgat tagctgctgt gtgtcttctc ggcggttgcg
     4381 atcagtcgag ggctcgagtc agagaatggg tctagttcgt acgtgccgta cagcttcggt
     4441 tcaacgaaac cggccaactc gagcatcaga agcagctcga ctggatagac gtatcgcaag
     4501 tcgaagcggg tatggattcg acccagctgg ccgacctccc cagagatgtc gtaccaaacg
     4561 ttcgtctgga tcacctgctc ggcccgttga atgacacgcg agctgaattt gctgactggt
     4621 cgtcctgctg gctcgttgtc ccagttccct tctagcacga cctggtgatc aacgaagtcg
     4681 atggccccgg agagtgggtt cggcaaatcg atgaatagct ggccccgggg atccagcacc
     4741 cggaaggcct cggccaggac ccggcgctgc tcgtcgagag tcgatgcgtg catgagcgtg
     4801 tttagtgcaa gcagaacgac gccgaatgtt tcatctggca gaccagtggt gctcatctca
     4861 gattcgacga gacgaacgtc ggccacaccg tcgagcctgg ctcgggcgcg ctccaacatt
     4921 gacggcgagt tgtcgattcc ggtcaatcgc atctcaaggt cggcgagatg cgcaagcacc
     4981 cgaccggtgc cgcaaccaag ctcaaggacc ggatcgccga ctgtctcgac gatgtagtgg
     5041 atcaagtcga ggtcgtcacg gaatctgttg tgctcaaggt cgtagagcgc cgcaatctcg
     5101 gtatacgggt gtgcgctcgg aagcgattga ctcaattccg gggcctctct atagtcacgg
     5161 aatgcgaaag acggcaggca ctgcaacgcg cgacgaacga cgggcggacc gggtggtcac
     5221 gtcgaaccgg cgtgccacgc atgattattt tatcgacgac acgatcgagg ccggaattgc
     5281 cttgcaaggg accgagatca agtcgatccg agaggggaag gtctcgatca ccgaggc
//
LOCUS       DATAOZ010000221         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6407036, whole genome shotgun sequence.
ACCESSION   DATAOZ010000221 DATAOZ010000000
VERSION     DATAOZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6407036"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VHR64_05885"
     CDS             <1..467
                     /locus_tag="VHR64_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HEX3302394.1"
                     /translation="GDQAAIGAGSVLDSETARIAILAGAQYIVTPTFKHATVELCNRY
                     GIPTVIGAFTATEMLTAQEAGADYIKVHPASLGGPKYFKDILAPLPFLKLIPSGGVTP
                     DNAAEFIRAGAVAVAMGSNLVDARTVANRDWDTITTRASSLSKTIAEARAGR"
     gene            complement(464..1075)
                     /locus_tag="VHR64_05890"
     CDS             complement(464..1075)
                     /locus_tag="VHR64_05890"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3302395.1"
                     /translation="MTARTAEAPLHDRARFTRIALALAVTLVLVAGAWVVGGREGFTN
                     IGGGGVNAKFLPRVGEVAPDLQAYRLNAQLQPELVSLSDFKGQPIWLNFWASWCQPCR
                     AEMPELKEAYQQLEGSGIVMLAISLDEPIEDAYLYAAQNQVTFMILSDPTREHVGSSY
                     QINNFPTHIFIDAQGIVRDVQLTPLSVAQALKSADLALHPTSG"
     gene            complement(1079..>1944)
                     /gene="ccsB"
                     /locus_tag="VHR64_05895"
     CDS             complement(1079..>1944)
                     /gene="ccsB"
                     /locus_tag="VHR64_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX3302396.1"
                     /translation="AATIALAGSTICYLFFAVGRVRVRRAAVATPEGATFTSTTTEYA
                     PGSEHFSRFATMLLWFTVFFQGLAVLTRTIAAGRGPYSNMYEFSLAFIFAITLIYALF
                     ERWYDVKQMGAIVAPVALGMAAYVWTLPGDLREVDGLIPALQHQPLMTAHVSSAIFAY
                     ATFAVSFAAAILFLIQQNRRVAWLPSPEMLDDIAFRAVTIGFPFLSLVLVLGSIWAYR
                     AWGSYWSWDPKETAALFTWLVYGVYLHTRSLRGWRGNRSAIILLFGFAAVIFTYYGNY
                     FFGGLHAYGGV"
BASE COUNT          407 a          579 c          631 g          327 t
ORIGIN      
        1 ttggcgatca ggcggcgatc ggtgctggat cggtccttga ttcggagacg gcgcggatcg
       61 cgatcctggc cggcgcccaa tacatcgtca caccgacatt caagcatgcg accgtcgagc
      121 tgtgcaatcg gtacgggatt ccgactgtca tcggtgcatt tacggcaacc gagatgctca
      181 cggcacaaga ggccggtgcg gactacatca aagtacatcc ggccagcctc ggcggcccga
      241 aatacttcaa ggacatcctg gctccgttgc cgtttctgaa gctgatcccg tccggaggtg
      301 tcacgcccga caacgcggct gagttcattc gggccggggc ggtggcggtc gcgatgggta
      361 gcaacctggt cgatgccaga accgtcgcca atcgcgattg ggacacgatc acaacccggg
      421 cgtcgagcct gagcaagacg attgccgagg cgcgggctgg tcgctaaccg gatgttgggt
      481 ggagcgcgag gtcggcgctc ttcagcgcct gcgcgaccga caatggcgtg agttgcacgt
      541 ctcgcacaat gccctgggcg tcaataaaga tgtgggtcgg gaagttgttg atttggtaac
      601 tcgacccgac gtgctcccgg gtaggatccg agaggatcat gaacgtgacc tggttttgcg
      661 ccgcgtagag atacgcatcc tcgatcggct catcgagact gatcgccagc atcacgatgc
      721 cggagccttc gagctgctgg tatgcctcct tgagctccgg catctcagct cggcagggct
      781 ggcaccatga cgcccagaag ttcagccaga tcggctgtcc cttgaaatcg gacagggaga
      841 cgagctccgg ctggagctgc gcgttgagtc gatacgcctg caggtctggt gcgacctcgc
      901 cgacgcgcgg caggaatttg gcgttgacgc cgccgccacc gatgttcgtg aagccttcgc
      961 gcccgccgac aacccatgcg ccggccacaa gaaccaatgt gactgccagc gccaacgcaa
     1021 tccgggtgaa cctggctctg tcgtggagcg gggcctcggc ggtacgagca gtcacgttct
     1081 agacgccacc atatgcatgc aggccgccga agaagtagtt gccgtaatag gtgaagatca
     1141 cggcggcgaa tccgaacagc agaatgatcg cgctccgatt gccacgccat ccgcggagcg
     1201 atcgggtatg caggtagact ccatagacaa gccacgtgaa gagtgcggct gtctccttcg
     1261 ggtcccatga ccagtacgaa ccccaggccc ggtaggccca gatcgaaccg agcacaagca
     1321 ccagcgaaag gaacgggaag ccgatcgtca ccgcacggaa ggcgatgtcg tcgagcatct
     1381 ccggactcgg caaccatgcc acccgccggt tctgctgaat caggaatagt attgcggccg
     1441 cgaacgagac tgcgaaggtc gcgtaggcaa agatcgcgct cgacacgtgg gcagtcatga
     1501 gcggctgatg ctggagcgcc ggaatcagac cgtcgacctc ccgcagatca ccgggcaatg
     1561 tccagacgta cgcggccatc ccgagggcga ccggcgccac gatcgcaccc atctgcttga
     1621 cgtcatacca gcgctcgaag agggcgtaga tgagcgtaat cgcgaagatg aacgctagcg
     1681 agaactcata catgttgctg tagggtccgc ggccggccgc gatggtgcgc gttagcactg
     1741 cgaggccctg gaagaagacc gtgaaccaga gcagcatcgt tgcaaagcgc gagaagtgct
     1801 cggagcccgg cgcgtactca gtggttgtgg aggtgaaggt ggcgccctcg ggtgtcgcaa
     1861 ctgccgcacg cctcacgcgg acccgaccaa cggcgaagaa gaggtagcaa atagtcgagc
     1921 cggcgagggc aatcgttgct gcgg
//
LOCUS       DATAOZ010000222         4448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1675095, whole genome shotgun sequence.
ACCESSION   DATAOZ010000222 DATAOZ010000000
VERSION     DATAOZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675095"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            604..1278
                     /gene="epsC"
                     /locus_tag="VHR64_05900"
     CDS             604..1278
                     /gene="epsC"
                     /locus_tag="VHR64_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEX3302397.1"
                     /translation="MWLREQIETIKQRDPAATSSLEVLLTYPGLHALIFHRASSAMRK
                     RDMRVAARLISHVGRFLTGIEIHPGAVIGRRLFIDHGMGVVIGETAIVGDDVTMYQGV
                     TLGGTGKQRGKRHPTVGNGVVIGAGAKILGAVTIGDGAKIGGGAVVLKDVPPYTTAVG
                     VPARAVAWTNPESGVSRRLEQLPDPQYDSMLAMIQRIEEQEDRIRALTAEVEEKESTL
                     AGSWQI"
     gene            1275..2669
                     /gene="cysS"
                     /locus_tag="VHR64_05905"
     CDS             1275..2669
                     /gene="cysS"
                     /locus_tag="VHR64_05905"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX3302398.1"
                     /translation="MTLRIYNTQSRTVEPFETIEPGVVRMYVCGVTPYAKAHIGHAMS
                     SIVFDVIRRYLTYRGYTVRHVTNFTDIDDKIIARANREGISPDELTEQMIAEWHRETA
                     ALNVLPADLYPRATQEVDRIITMIEELIRQGFAYECEGDVYYRVRAFEEYGKLSHRSL
                     DDLLSGARIEIDERKEDPLDFALWKAAKPGEPWWESPWGKGRPGWHIECSAMCSHHLG
                     GLVDIHGGGADLIFPHHENEIAQSEAALQQEPFARYWVHNGLLQLGGEKMSKSIGNLV
                     AVDELIAQKRTAAFRVLVLQSHYRAPLTFTDDGLEAAQRGLDRIRAALNPAAIATDSV
                     PLQPGIADETRAEFEAAMDDDFDTPKAMAAIFNLARAINRSAGNPDSAEALEASRQTL
                     QSLLDVFGIAPDADEESEMIGAAPFIDLLLRIREQLRAGKHWAEADAIRNGLNELGIR
                     IEDGPEGATWRQES"
     gene            2626..3450
                     /gene="rlmB"
                     /locus_tag="VHR64_05910"
     CDS             2626..3450
                     /gene="rlmB"
                     /locus_tag="VHR64_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX3302399.1"
                     /translation="MRTGPKAPPGDKRVKPRRPGRDEGKDRIYGRNAVVAVLHGKRHV
                     HGILLAEGVGQDSRIATIRELAERRRIQIQTVPVQGIENIVGKVNHQGVLATTDRFGY
                     VALRTILDRPGTVLILDHVTDPQNLGTLLRAARAFEIAGVVIPSDRAASVTPAVVNAS
                     AGAVESLPVAQVVNINRAITEIREHGRWVAALDTGSDSTSIFTTEIPLPVALVLGSEG
                     RGVSKQVRASSDLVISIPISSDVESLNVATAGSIVLFELARRMHGSSSGRVSERLN"
     gene            3573..4058
                     /gene="rplI"
                     /locus_tag="VHR64_05915"
     CDS             3573..4058
                     /gene="rplI"
                     /locus_tag="VHR64_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX3302400.1"
                     /translation="MKIILRKDVEKLGEAGSLATVKDGYARNFLIPQGMAVAATPGEL
                     KMWEHNQAVKARKIERQERELQSFADKIAAVTLSFEARAGEGGRLFGSVTAADIAERL
                     TKEVGEEIDRRKVVLTEPIRSTGEHTVTVNVIGKLRPQVKVIVNGVVDDSEEDVADAA
                     E"
     gene            4136..>4448
                     /locus_tag="VHR64_05920"
     CDS             4136..>4448
                     /locus_tag="VHR64_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEX3302401.1"
                     /translation="MADRLPPQNLEAEQSVLGSLLIDRDAIIQVAPILKHEDFYQPSH
                     GTIYQAINELYTRQEPTDVITLTDELARRDKLDLVGGASYLTSLVSVVPTAVHVEYYA
                     RI"
BASE COUNT          876 a         1348 c         1419 g          805 t
ORIGIN      
        1 aactgggatg accagtccga tttcgatccg gcctgatccg cgagtggata ttgagcagac
       61 ctgcctccct gactagaatt agtcgattac aagttcgatc tttgtacctg tttgggacat
      121 tgcatgccta cgtatcagta tcagtgccga gattgcggcc acgcgtttga gcagtttcag
      181 aaattcagcg aggatccgct caccgattgc cccgcctgcg cggggtcggt acagcgggta
      241 atccagaatg tcggcgtcgt attcaagggc tccggctggt atatcaatga cagccgcaag
      301 tcgaacgggt ccaatggctc gagcgattcc aagccgacct cggaatctcc cgatactgcg
      361 accaagacca cgtccaaaac cgatggtgag aaggctccgg ccgcggcccg cgccactacc
      421 aagtccgact agccggagga ttcgcgagcg aacatgaacg cccttcgcca aatgtgctgg
      481 ttccatcctg agcgatgcgg cgcggcagtt ggcggccctt gctcctgttc cggctgcgca
      541 cgctagaacg ctgcgcagaa acagaggcga ccggttcaga tttcaggcaa gaagggcaag
      601 aacatgtggc ttcgcgagca gatcgagact atcaagcaac gtgatccggc agcaacctcc
      661 tcactcgagg tgttgctgac atatccgggg ttgcacgcgt tgatctttca ccgtgcttcc
      721 tctgcgatgc gcaagcggga tatgcgggtc gctgcccgcc tcatctcgca cgttggacgg
      781 ttcctgactg ggatcgagat tcatcccggc gcggtgatcg gccggcgcct attcatcgac
      841 cacggcatgg gcgtggtcat cggtgagacc gcgatcgtcg gcgacgatgt cacgatgtac
      901 cagggcgtga cattgggcgg cacgggcaag cagcgtggca agcgccaccc cacagtgggc
      961 aacggtgtcg tgatcggcgc cggagccaag atcctcggcg cggttacgat cggtgacggc
     1021 gcgaagattg gtggcggggc ggttgtcctc aaggacgtgc ccccgtacac gaccgctgtc
     1081 ggggtgccgg cgcgggcggt cgcatggact aacccggaat ccggcgtctc gcgacgactc
     1141 gagcagctcc cggatccgca atacgactcg atgctggcca tgatccagcg gatcgaggag
     1201 caggaggatc ggattcgggc gctgacggct gaggttgaag agaaggagag cacgctggcg
     1261 gggtcgtggc agatatgacg ttgcgcatct acaacaccca gtcgcgcacc gtcgaaccat
     1321 ttgagacgat cgagccgggt gtggtcagga tgtacgtctg cggcgtcaca ccgtacgcca
     1381 aagcgcacat cggccacgcc atgtcgtcga tcgtgttcga cgtgatcaga cggtatctga
     1441 cctatcgcgg atacaccgtc cggcacgtca cgaacttcac cgacatcgac gacaagatca
     1501 tcgcgcgggc gaaccgcgaa gggatcagcc ctgacgagct gaccgagcag atgatcgccg
     1561 aatggcaccg ggagaccgcc gcgctcaacg tgctgccggc cgatctctat ccgcgggcga
     1621 ctcaggaagt cgaccggatc atcacgatga tcgaggagct gatccggcag ggattcgcct
     1681 acgagtgcga gggcgatgtc tactatcggg tccgcgcctt cgaggagtac ggcaagctat
     1741 cgcaccgcag ccttgacgac ttactctcgg gagcccgaat cgaaatcgat gagcgcaagg
     1801 aagacccgct cgatttcgcg ctctggaagg cggccaagcc cggcgagccc tggtgggaat
     1861 cgccatgggg gaaaggccga ccgggctggc acatcgagtg ctctgcgatg tgctcgcatc
     1921 atctcggtgg gctggtcgac atccatggcg gcggggccga cttgatcttc ccgcaccacg
     1981 agaacgagat tgcccagtcg gaagcggccc tccagcagga gccgttcgcc cgctattggg
     2041 tgcacaatgg cctgcttcaa cttggtggcg agaagatgtc caagtcgatc ggcaacctgg
     2101 tcgcggtcga tgagctcatc gcgcagaagc gtacggctgc attccgggtc ctggtcctgc
     2161 aatcgcatta ccgcgcgccg ctgacattta ccgatgatgg cctcgaagcc gcgcagcgtg
     2221 gtctcgaccg catccgagcc gccctgaacc cggctgcaat cgcgaccgac tccgttccgc
     2281 ttcaacccgg catcgcggac gaaacgcgcg ccgagtttga ggcggcgatg gacgacgact
     2341 tcgacacgcc gaaggcgatg gccgcgatct tcaacctcgc tcgcgcaatc aaccgatccg
     2401 cgggaaaccc ggattcagcc gaggcgctcg aggcatcgcg gcagacgctt cagtcgctgc
     2461 tcgatgtgtt cggtatcgcg ccggatgcag acgaggaatc ggagatgatc ggcgccgcgc
     2521 cgttcatcga cctgctcttg cggatacgag agcaattgcg ggccggaaag cactgggcgg
     2581 aagccgacgc gatccgaaat ggcttgaatg aattggggat acgaattgag gacgggcccg
     2641 aaggcgccac ctggcgacaa gagagttaag ccgcgccgcc caggtcgcga cgaaggcaag
     2701 gaccgcatct acggcaggaa cgctgtcgtc gcggtcctgc acggcaagcg ccacgtccac
     2761 ggaatcctgc tcgccgaggg cgtcgggcag gattcgcgga tcgcgaccat ccgggagttg
     2821 gcagagcggc ggcgtataca gatccaaacg gtgccggtcc agggtatcga gaacatcgtc
     2881 ggcaaggtca accaccaggg cgttctggcg acgacagacc gcttcggata cgtcgcactg
     2941 cggacgatcc ttgatcggcc ggggacggtc ctgatcctgg accatgtcac cgacccgcag
     3001 aatctgggca cgctgcttcg tgcagcgcgt gcattcgaga tcgcgggcgt ggttattccg
     3061 tccgaccgtg ccgcgtcggt gacgccggcc gttgtcaatg cctccgcggg cgccgtcgaa
     3121 tcactccctg tcgcgcaggt cgtcaacatc aaccgggcca ttaccgagat tcgcgagcat
     3181 ggccggtggg tggcggcgct cgataccgga tcggacagca cgtcgatctt cacgaccgag
     3241 attcccctgc cggttgcgct cgtgctgggg tcagagggtc gcggagtcag caagcaggtc
     3301 cgcgcatcca gcgatttggt tatctcaatc ccgatcagca gcgatgttga gtcactcaac
     3361 gtggcgactg ccggatcgat cgtcctgttc gagcttgcgc gacgcatgca tggcagttcc
     3421 tcgggccggg tttcggaacg tttgaattga tttcgaccga ttgagcctct agaattggtt
     3481 ggccggaccg cggtccgtcc tatgttgtgt ctcaaaactg gcgcggattc ggcgccaacg
     3541 ccagccccag gcattggaag gttccgttca cgatgaagat cattctgcgt aaagatgtcg
     3601 aaaagctcgg cgaggccggc tccctggcga ccgtcaagga tggctacgcg cgcaatttcc
     3661 tgatcccgca gggcatggcc gtggccgcca cgcctggcga gctcaagatg tgggaacata
     3721 atcaggccgt caaggcgcgg aagatcgagc gacaggagcg cgagctccag tccttcgccg
     3781 acaagatcgc cgccgtgaca ctgtccttcg aggcccgcgc cggtgaaggt ggtcgcctct
     3841 tcggctccgt caccgctgcg gacattgccg agaggttgac gaaggaagtc ggcgaagaga
     3901 tcgatcgccg caaggttgtc ctgaccgagc cgatccgatc gaccggcgag cacacggtta
     3961 ccgtcaatgt gatcggcaag ttgcggccac aggtcaaggt catcgtcaac ggcgttgtcg
     4021 atgattcgga agaggacgtc gcggacgcgg cagaataact cgtcagcctg accccgccag
     4081 gtgacgctgg gatcgttgct cccggtcgga gcatgttgtt tccaggttgg ggtagatggc
     4141 agatcgtctt ccaccgcaaa atctagaggc cgagcaatct gtgctcggta gcttgctgat
     4201 tgatcgcgat gccatcattc aggtcgctcc tatcctcaag cacgaggact tctaccagcc
     4261 ctcgcacggg acgatctacc aggcgatcaa cgagctctac acgcgccagg aaccgaccga
     4321 cgtcatcacc ctgacggacg agctggccag gcgcgacaag ctggacctcg tcggcggtgc
     4381 ctcgtacctg acatcgctcg tctcggtcgt gccgaccgcc gtccacgtcg agtattacgc
     4441 ccggatcg
//
LOCUS       DATAOZ010000223         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2461314, whole genome shotgun sequence.
ACCESSION   DATAOZ010000223 DATAOZ010000000
VERSION     DATAOZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461314"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..2682
                     /locus_tag="VHR64_05925"
     CDS             274..2682
                     /locus_tag="VHR64_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3302402.1"
                     /translation="MAAEPSVQHIHGYCGLCISHCGSIATVQNGRFIALEPDPSHPTG
                     KALCAKGRAAPEIVYHPDRLRYPMKRTRPKGDPDPGWQLITWDEALDVTAARLRELAE
                     RHGPQSVIFSSASPSTSACADSVHFIQRLMRAFGSPNLCASMELCGWGRASTIYTYGM
                     PSGLGTGSLMPDLEHAECILYWGYNPSIARISHATSTVAALKRGARLIVVDPRRVGFA
                     NKADVWLRVRPGSDGALALGIAGVMIERGWYDHDFVRNWTNGPLLVREDNGRLLTTSD
                     LGHAGNSKRYVARDEVSSGLILYDPATRRFDGDHAVPALLGVYSVSTPDGTIICRPVF
                     QLVADLCRQYSPERVEEICWVPAEQVEAAARLLWEARPVASFAWSGVEQHTNVTQFAR
                     ALALLYSLTGSLEAEGGNVVFPVVPQGSVDGREFVAPEQASRNLGIRDRPLGPGRMHF
                     VTTDELYRGIQEHDPFAVHGLVGFGANLLMAHSESARGRVALAALDFYVHADLFMSPT
                     AEMADIVLPVATPFERESLEIGFGSSAEGSRLVQLRPAVVPPLGESRPDTDIVFDLAC
                     RLGLGAQFWEGDVDAAYRHQLGPTGITLEMLRANPAGVRVPLQMPYRRYAQPGEDGTT
                     RGFMTPSGKVELYSETFLEHGYSPLPGYEEPLVSPISRPDLAARFPLILTSSKSAAFC
                     ESQHRSIPSLRKRARDPEIELNPETATARGIVEGDWVTIETPEGLVRARATLNETLEP
                     RVVCGQHGWWQACPQIDAPGYDPFSSSGANLNLIIGNDAVDPVSGSVPHRSYLCEVRP
                     APSA"
     gene            2760..3176
                     /locus_tag="VHR64_05930"
     CDS             2760..3176
                     /locus_tag="VHR64_05930"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX3302403.1"
                     /translation="MTPDELKDCARRFFAEIWNARDESAIDRFIPENAKGNDADFGSG
                     REGFRKQWREWMVAFPDLHFEIVDMVAEGDKVLTRWVLTGTHSDAPFLGVEPTGTKIR
                     VEGMSLDRVEDGWVAEGFDGWDALALRRQLGIYDGA"
     gene            complement(3421..4737)
                     /locus_tag="VHR64_05935"
     CDS             complement(3421..4737)
                     /locus_tag="VHR64_05935"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302404.1"
                     /translation="MAGPHVAVSESGGIIGGRQRRYRRSYFALISSSFVSNTGNAFSN
                     LAIPLFVLATTGSATRTGIVAFVNYAPPVVAAIFGGALVDRIGRRRTLMTADALSCIA
                     TALIPLCYMADLLSFPLLLALVAFGAFLDSPGRAARTSMIPALADGAGYSPERAQSLS
                     MSGFFLSQVIGPALAGLVVAASGPTTAIWVNAATFVVSLLIVKNVVQEPEINVPANES
                     TYLEDLREGFHFVWHDGFMRAMIILASAFSMVFIPLYTVLYPVYFTRLIGSTRALGFF
                     LGAEAAGNLIGAVAYGYIGERFSRFHAFIFSMAAWVPFFAVMLFTPPVWLILVSGFVA
                     SLVSGAINPIFQVAFQVRTPEAMRPRVYSMVTAGNLMAVPVGALLIGPLIELIGVIPT
                     LGIVVAMGAAAPIVCYLVPVFREIDHEIEITEPAPAADERAFEPVA"
     gene            4885..>5438
                     /locus_tag="VHR64_05940"
     CDS             4885..>5438
                     /locus_tag="VHR64_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302405.1"
                     /translation="MRDGDRLDDSSSCVPSSRAHSRRLFIAAAGATLLALPTAAHASP
                     SASHHTEPAHHQDLKPLLKRLRKRTVSEDMVLAVAEALARSGIGTYASPADTEPIVAI
                     SGTPSPLKYLRWQVTTMANELAVRNGSTGAELDSLIDPFESGVSPFVIIAGYVREGKS
                     TGADLVRGLLGKRNWQHRDFRIPML"
BASE COUNT         1010 a         1801 c         1724 g          903 t
ORIGIN      
        1 ccgggaacca cgcgcgcgta ttcatactcc gcaactccgg gctccgcctt ccctgatgcc
       61 tcagtgaagg gtctccgacc gcacataccg ccccgccagc attgtccgaa tccacccgcc
      121 caacctggaa cactgaagcg ccggtcccca tggcaaatcc gcaacaaatt ctgactggcg
      181 gcgcgatccc tcgcccccaa aaacccgctt gcggtgcgtt gctaggatgg aaacaccaga
      241 gcgtcagcgc ggctaattcg aaggagcgcg tacttggctg ccgagccgag cgtccagcac
      301 atacacgggt actgcggact gtgcatttca cattgtggat caattgccac ggtgcagaac
      361 gggcggttca tcgcgctcga gccagaccct tcgcatccga ccgggaaggc actctgcgcg
      421 aagggtcgcg cggctccgga gatcgtctac caccctgacc gactgcgcta tccgatgaag
      481 cggacacgtc ccaagggtga ccccgacccg ggttggcagc taatcacctg ggacgaggct
      541 ctcgacgtga ccgcggcgag gctgagggaa ctggcggagc gccatggtcc gcagagcgtc
      601 atcttcagca gcgcctcgcc gtcgacctct gcctgcgccg actcagtgca cttcatccag
      661 cgcctgatgc gcgccttcgg cagcccgaac ctctgcgcct cgatggagct gtgcggttgg
      721 gggcgagcct ccacgatcta tacctacggc atgccatcgg gcctcggcac aggctccctg
      781 atgccggacc tggagcacgc cgagtgcatc ctctattggg gatacaaccc gagcattgcg
      841 cggatctccc acgccacgag caccgtcgcg gccctcaagc gcggcgcgcg gttgatcgtg
      901 gtggacccgc ggcgggtcgg cttcgcgaac aaggccgatg tctggctgcg ggtgcggccc
      961 ggctcagatg gcgcactcgc actcggcatc gcgggcgtca tgatcgagcg cggctggtac
     1021 gaccatgact ttgtccggaa ctggaccaac ggcccgctcc tcgttcgcga ggacaacggc
     1081 cgcctactga ccacctctga tcttggccac gccggcaatt cgaagcggta tgtggccagg
     1141 gatgaggtgt cgagcgggct gattctctac gacccggcaa cgcgccgctt cgacggcgat
     1201 catgccgtcc ctgccctcct cggcgtatac agcgtcagca caccggatgg cacgatcatc
     1261 tgccggcccg tgttccagct cgtggccgat ctttgccggc agtactcgcc ggagcgggtg
     1321 gaggagatct gctgggtgcc cgccgagcag gtcgaggcgg ccgcgcggct gctctgggag
     1381 gcgcgaccgg tcgcctcctt cgcgtggagc ggagtcgagc aacatacgaa cgtgacccag
     1441 ttcgcgcgcg ccctggcact cctctactcc ctgaccggct cactcgaagc cgagggcgga
     1501 aatgtcgtct tcccggtggt tccacagggc tcggtcgacg gacgggagtt cgtcgctccg
     1561 gagcaggcgt cccgcaacct cgggatcagg gaccggccgc tcggtccggg ccggatgcac
     1621 tttgtgacga cggacgagct ctaccggggc attcaggagc atgatccctt cgccgtccat
     1681 ggcctggttg gattcggcgc gaatctcctg atggcccatt cggaaagtgc gcgaggccgg
     1741 gtggcgctgg cggcgctcga cttctacgtc catgccgatc ttttcatgtc gccgacggcc
     1801 gaaatggccg acatcgtgct tccagtggcg acgccattcg agcgggaatc gctcgaaatc
     1861 ggattcggca gcagcgcgga gggaagcaga cttgtccagc tccggccggc tgtggtcccg
     1921 ccgctcggag aatcccggcc cgataccgat atcgtcttcg acctcgcctg ccgcctcggt
     1981 ctcggcgcgc agttctggga gggtgacgta gatgccgcgt accgtcacca acttgggccg
     2041 accggaatca cgttggagat gctccgtgcg aaccctgccg gagtgcgggt gccgctgcag
     2101 atgccctacc gccgatatgc ccagcccgga gaggacggga ccactcgcgg gttcatgacc
     2161 ccgagcggca aggtcgagct ctattcggag accttcctgg agcacgggta ttcgccgctg
     2221 ccgggctacg aggagccgct cgtcagcccg atctcccggc cagatctcgc cgcgcgcttc
     2281 ccgctcatct tgacctcgtc caagagcgct gccttttgcg agagccagca ccggagcatt
     2341 ccaagcctcc ggaagcgagc ccgcgatccg gagatcgagt tgaacccgga aacggccacc
     2401 gcgcgtggca ttgtcgaagg agactgggtg acgatcgaaa cgcccgaggg gttggtccgg
     2461 gcgcgcgcaa cgttaaatga gacccttgag ccgcgcgtcg tctgcggcca acacggctgg
     2521 tggcaggcgt gcccgcagat cgacgcgccg ggatacgatc cgttctcttc gagcggcgcc
     2581 aatctcaacc tgatcattgg gaacgacgcg gtcgacccgg tcagcggctc ggtcccgcac
     2641 cgctcctacc tttgcgaggt gcgtccagcg ccctcggcgt gactccagca gaaacagccg
     2701 cacgttgtcg gctataaagg cgcaacgttt cgatgtgctg gatgccgatg gaggggaacg
     2761 tgacaccgga cgagttgaag gattgtgcgc ggcggttttt tgccgagatc tggaacgcgc
     2821 gggatgagtc cgcgatcgat cgtttcatcc ccgagaatgc caagggcaac gacgccgact
     2881 tcggcagcgg tcgcgaaggt ttccggaagc agtggcgtga gtggatggtg gccttcccgg
     2941 acctgcactt cgagatcgtc gacatggtcg cggagggcga caaagtcctg actcgctggg
     3001 tcctgaccgg cacccacagc gacgcgccgt tcctgggcgt cgagccgacc ggcacgaaga
     3061 tccgggtcga ggggatgagc ctggatcggg tcgaggatgg ctgggtcgcc gagggtttcg
     3121 acggctggga cgcgctcgcc ctccgccgcc aactcggcat ttacgacggc gcctgaccgc
     3181 tacctcgacc agggcggcga ggcttcttca cctttcgcgt ggaagaaccg aattccggcc
     3241 ggggcaagag gagagtgccg gcgagaggac gacgcccgcc cggtcccgat gcccgtcaac
     3301 ccggccggga actgatcccg gtacagcccc ggtgccatta ctcgtctgtg aggatgctcc
     3361 aagcggggcg tggtaaaccc gcgctacatc aacgaccgga ccaaccttct gaacttcgtt
     3421 ttaggctacc ggctcaaacg cgcgctcgtc agccgccggc gccggttcag tgatctcgat
     3481 ctcgtgatca atctcacgga aaaccggcac caggtagcag acgatcggcg cggccgctcc
     3541 catcgcgacc acgatgccaa gcgtcgggat cacgccgatc aactcgatca gtgggccgat
     3601 tagcagcgct ccgaccggca ccgccatcag gtttccggcc gtgaccatgc tgtagacccg
     3661 cggccgcatc gcctcgggcg tccgcacctg gaaggcaacc tggaagatcg ggttgatcgc
     3721 gcccgacacc agactcgcca cgaacccgct gaccaggatg agccaaaccg gcggagtgaa
     3781 gagcatcacc gcgaagaacg gcacccaggc tgccatgctg aaaatgaagg cgtggaaccg
     3841 gctgaaccgc tcgccgatgt acccatatgc caccgcgcca atcaggttgc cggccgcctc
     3901 cgctcccaaa aagaaaccga gcgctcgggt gctgccgatc aaccgcgtga aatagaccgg
     3961 gtagagcacg gtgtagagcg ggatgaagac catcgagaaa gcggaggcga ggatgatcat
     4021 tgcccgcatg aagccgtcgt gccagacgaa gtggaagccc tcgcgcaggt cctcgaggta
     4081 ggtcgactcg ttggccggga cattgatctc cggctcctgc accacgttct tgacgatcag
     4141 cagcgagacc acgaacgtgg cggcattgac ccaaatcgcc gtcgtcgggc cggacgccgc
     4201 cacgaccagg ccagccagag ccggtccgat cacctgggac aggaagaagc cggacatgct
     4261 cagcgactgc gcgcgctcgg gtgaatatcc ggccccgtcc gcgagcgccg ggatcattga
     4321 cgtgcgggcc gcccgccccg ggctgtcgag gaatgcgccg aacgcgacca gcgccagcaa
     4381 gagcggaaaa ctgagcagat cggccatgta acagagcggg atcagcgcgg tcgcgatgca
     4441 gctcagtgca tcagccgtca tcagcgtccg gcggcggccg atgcggtcga ccagtgcccc
     4501 gccgaagatc gcggctacca ccggcggcgc gtaattgacg aatgcgacga tacctgtccg
     4561 ggtcgcactg ccggtcgtgg ccagcacgaa gagcggaatc gccaggttcg agaaggcgtt
     4621 cccggtgttc gagacgaatg aggatgagat cagcgcgaaa taggaccgtc ggtaacgacg
     4681 ctgcctgccg ccgatgattc cgccagattc cgaaactgca acgtgagggc cagccaagcg
     4741 cgtgcgatcc cttccaaccg gcagaaccta atcgctcgac cgtgatttcg tcaactgaca
     4801 aatgacccat aggcaaagtg cgtcgcgcgc gctagcattc gctcacccgc tcgggaaacg
     4861 aatgtctata gaagggctgc gccaatgcgc gacggggatc gcctcgacga ctcatcatct
     4921 tgtgtaccgt catcccgcgc ccattcccgc cgcttattca ttgccgccgc aggggccacg
     4981 ctcctggccc ttccgaccgc cgcccacgcc tcgccatcgg cctctcacca cactgaaccg
     5041 gcacatcacc aggacctgaa gcctttgctc aagcggcttc gcaagcgcac cgtttccgag
     5101 gacatggtcc tggcagtggc cgaagccttg gcacgctccg gtatcggaac ctatgcaagc
     5161 ccggccgaca cggagccgat tgttgcgatc agcggaacac catcgccgct caagtacctg
     5221 cgatggcagg tcaccacaat ggcgaatgag ctcgccgtcc gcaatggatc cactggagcc
     5281 gagctggatt ccctgatcga tcccttcgag agcggggtca gtccctttgt catcattgcg
     5341 ggatatgtcc gtgaggggaa atcgaccggc gccgacctcg tccgtggctt gctcggcaag
     5401 cgcaattggc aacatcggga tttccggatc ccgatgct
//
LOCUS       DATAOZ010000224         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4830289, whole genome shotgun sequence.
ACCESSION   DATAOZ010000224 DATAOZ010000000
VERSION     DATAOZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4830289"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1623
                     /locus_tag="VHR64_05945"
     CDS             <1..>1623
                     /locus_tag="VHR64_05945"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX3302406.1"
                     /translation="EHSQNVYKRAIEQGFHNNPVGYTDRMEKEFRAVWSQLNLSFDDF
                     IRTTESRHRTAVQKLAQVSYDRGDIYEGTYEGWYCVSCEAFKQEKDLVDGRCPLHPTL
                     TPEWIREKNFFFRLSKYRDALLKHFADQPTFLEPDVRRNEILRLLEGGLEDISMSRAG
                     QSWGIPLPFAPDNVVYVWFDALINYIAAVGYGTDEEKFATWWPANLHVIGKDITRFHT
                     VVWPAMLMSAGVPLPKQVFGHGWVNYGGQRMSKSLGTSVDPVDVVARFGPDPLRLYLT
                     KEISYGSDGDFTWERYEERYNADLANNLGNLVSRLAAMAEKYRGLQLKAVASGGRLPQ
                     VVEAAVTAYRAAMERFALHEAAAAAFQIVDRTNEFIAETAPWKLAKDSADAGRLDGVL
                     FDAAEAIRIAAVLLLPIMPESAAEILRRMGEATPVDRIRFESGARWNHNGDRQIVKGD
                     ALWPRAEERPKEIAVDEATKTADVPAPQAAATPAPPPAPGPSSTPSGDNRIAIDDFMK
                     VELRVAKILEAEAVPKSKKLIKMKVNAGEAEPR"
BASE COUNT          324 a          488 c          546 g          265 t
ORIGIN      
        1 gagcactcgc agaatgtcta caagcgggcg atcgagcagg ggttccacaa caacccggtc
       61 gggtacaccg accggatgga gaaggagttc cgggcagtct ggtcgcaatt gaacctgtcg
      121 ttcgacgact tcatccggac caccgagtcc cgccatcgca ccgcggtaca gaagctggcg
      181 caagtcagtt atgaccgggg cgacatctac gaggggacgt acgagggctg gtactgcgtg
      241 tcctgcgagg ccttcaagca ggagaaggac ctggtggatg ggaggtgccc gctgcacccg
      301 acgctgacac cggaatggat tcgcgagaag aacttcttct tccggctgtc gaagtatcgc
      361 gatgccctgc tgaagcattt tgccgatcag ccgacgttcc tggaaccgga cgtgcgacgc
      421 aatgagatcc tgcgcttgct cgagggcgga ctcgaggaca tctcgatgag tcgcgccggt
      481 caatcgtggg gcatcccgct gccgttcgct cccgacaatg tcgtctacgt ctggttcgac
      541 gcactcatca actacatcgc cgccgtcggg tacggaacgg acgaggagaa gttcgcgacc
      601 tggtggccgg ccaacctgca tgtgatcggc aaggacatca cgcgattcca caccgttgtc
      661 tggccggcca tgctgatgag tgcaggcgtg ccgctaccca agcaggtgtt cggccacgga
      721 tgggtcaact acggtggcca gcgcatgagc aagtcgcttg gcaccagcgt ggatcccgtc
      781 gacgtcgtgg cgcggttcgg accggatccg ctgcggctgt atctcaccaa ggagatctcg
      841 tacggcagtg acggcgactt cacctgggag cggtacgaag agcgctacaa cgcggatctc
      901 gccaacaatc tgggaaatct ggtcagccgg ctggcggcga tggcggagaa gtatcgcgga
      961 ctgcagctga aggcggtggc aagcggcggc agactgccgc aggtagtcga ggcagccgtg
     1021 acagcgtatc gcgcggcgat ggagcgcttc gcgcttcatg aagccgcggc cgccgcattt
     1081 cagatcgtcg accggacgaa tgaattcatt gccgagacgg ccccctggaa gctggcgaag
     1141 gattccgccg acgccgggcg cctggacggt gtcctgttcg acgccgcgga ggcaatccgg
     1201 atcgccgcgg tgctgctgct gccgatcatg ccggaatcgg ccgcggagat tctgcgccgc
     1261 atgggcgagg cgacacctgt cgatcgcatt cggttcgaat ccggtgcccg ctggaaccac
     1321 aacggagacc gacagattgt gaaaggcgat gcgctctggc cgcgagctga ggagcgcccg
     1381 aaggagattg cagtggacga agcgaccaag accgcggacg tgcctgcgcc gcaggcggcg
     1441 gcaaccccgg cgccgccacc ggcgccgggg ccatcctcga ctccatccgg cgataaccgt
     1501 atcgccatcg acgacttcat gaaggtagaa ctacgcgtcg ccaagatcct cgaggcggag
     1561 gcggtcccga agtcgaagaa gctgatcaag atgaaggtga atgccggcga ggccgaaccg
     1621 cgc
//
LOCUS       DATAOZ010000225         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3777475, whole genome shotgun sequence.
ACCESSION   DATAOZ010000225 DATAOZ010000000
VERSION     DATAOZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3777475"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VHR64_05950"
     CDS             complement(<1..214)
                     /locus_tag="VHR64_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302407.1"
                     /translation="MIRVLESVDRRERVVSNPLADPAFVSEPPTGNPLAVHKRMPGYQ
                     PSPLIELPVIARELGLERLWLKDESSR"
     gene            complement(211..1884)
                     /locus_tag="VHR64_05955"
     CDS             complement(211..1884)
                     /locus_tag="VHR64_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302408.1"
                     /translation="MAELVRLDGNLVVEGGGEHPHALRRLRASLPGTVIGGGSAPDGA
                     TTVRLVAATERAEGSFAIHVSGGEAPVVEVEGGPFSGVIYGVEELVQRQLTEGAVQVG
                     TVEQAPGLPYRTFWNWDHTTNWEMTQIGVQEIGVMNPYHKPPEGFLADFKRVVDFMSM
                     NRIAAIAIYGFLRDSHGGIEAAQELCRYANERGVRIMPGVAINAYGGVVWEMDHDYNL
                     ANWLRRHPELAAQMERPAGFKIDDLAFPLFFPIGDYSVRGCPSKVENQRWMEEGIAWL
                     AETFEVGGINIEAGDYGVCGCPDCRARRAAREDANRRQGYAESWSHADMADFYPRLYD
                     AALSKRPNAWIYSEIQWDNLLDADAMQPLRALPEGGIYQHTTNRSYWKRVMAEMTPEY
                     TKSLPTKTNVFRTQFNSQWNGDYRTERYRFNAKDFAEQAKKAAEVGFQGLTVWGEPSP
                     YHVSSELSYLAFARFTWDPGLTWEQFMREEVAPRLGGEAAAERFVELVETLDGSADLD
                     AAQLERIRTESVSNAAGTAGDASRRWLWLAEQTTRRQYSQADRPEGSPW"
     gene            2063..2314
                     /locus_tag="VHR64_05960"
     CDS             2063..2314
                     /locus_tag="VHR64_05960"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEX3302409.1"
                     /translation="MATHIDRREDVSPKEGLHEYGDVKFADETNKKYPIDTKEHIRAA
                     WSYINHKDNAAKYDKDEVKAIKGRIKRAAIDKGVDIESD"
     gene            2499..2990
                     /locus_tag="VHR64_05965"
     CDS             2499..2990
                     /locus_tag="VHR64_05965"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3302410.1"
                     /translation="MELLDRLLGHDQWTTERCLELCRDLNEDQLDQPFDIGHRTLRAT
                     FAHMIYNVGFWTSLMTGDTPAANPESPSLAEMTEVHERTYLVFADLANRLCGDGRLDE
                     TFTDHYGITQTFGATIIQVILHNHQHRSDVLHILQRLGVPDLPDGDPQEWEHLTGRTP
                     WRS"
     gene            complement(3187..>3578)
                     /locus_tag="VHR64_05970"
     CDS             complement(3187..>3578)
                     /locus_tag="VHR64_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3302411.1"
                     /translation="TYPDRCRVQIERRTVPGEMDAMVEQQLRELIGDAADLTMGISRQ
                     PFEIGPEAELVQAMQAVVTQRLGQPTEIVGWGGWMDSALTSAAGIPSLVFGPTGHGAH
                     ADNEWIDLDSVQTCREIYADLARSWSA"
BASE COUNT          684 a         1238 c         1046 g          610 t
ORIGIN      
        1 tccggctcga ctcgtccttg agccaaagcc gctccagtcc cagctcgcgc gcgatcaccg
       61 gcagctcaat cagcggcgac ggctgatacc ccggcatccg tttgtgaacg gccaacggat
      121 ttccggtcgg cggctcactg acgaacgcag gatcggctag cgggttggaa acgacccgct
      181 ccctccgatc gaccgactcc aaaacccgga tcaccacggc gatccttccg gtcgatcggc
      241 ctgactgtac tgtcgccgcg tcgtctgctc ggcaagccag agccaccgtc gcgaagcatc
      301 gccggccgtg cccgccgcat tcgaaaccga ttcagtgcga atccgctcga gctgcgcagc
      361 atcgaggtcg gccgacccgt ccagcgtctc gaccaactcg acgaagcgct ctgccgccgc
      421 ctcaccgccg agccggggcg cgacctcctc ccgcatgaac tgctcccacg tcaggcccgg
      481 atcccatgtg aaccgtgcga aggccaggta gctcagctcc gatgagacgt ggtatggcga
      541 cggctcgccc cagacggtca gcccctggaa tccgacctcg gccgccttct tcgcttgctc
      601 cgcaaaatcc ttcgcattga accggtagcg ttcggtccgg taatcaccgt tccactggga
      661 gttgaactgc gtccggaaga cgttggtctt cgtcggcagc gacttggtgt actccggcgt
      721 catctcggcc atgacgcgtt tccagtacga gcggtttgtc gtgtgctggt agatcccacc
      781 ctccggcagc gcccggagcg gctgcatcgc gtcggcgtcg agcaggttgt cccactggat
      841 ttcggagtag atccaggcgt tgggccgctt cgagagcgcg gcatcgtaga gccggggata
      901 gaaatcggcc atgtccgcgt gcgaccagct ctcggcgtac ccctgccgcc ggttcgcatc
      961 ctctcgcgcc gcgcgccggg cccggcagtc ggggcagccg cagacaccgt agtccccggc
     1021 ttcgatgttg atcccgccga cctcgaacgt ctcggccagc caggcgatac cctcttccat
     1081 ccagcgctgg ttctcgacct tggacgggca gccccgcacg ctgtaatcgc cgatcgggaa
     1141 gaaaagcggg aaggcgagat cgtcgatctt gaaaccggcc ggtcgctcca tctgcgccgc
     1201 cagctcgggg tgcctgcgga gccagttcgc caggttgtag tcgtggtcca tctcccagac
     1261 cacgccgccg taggcattga tcgcgacgcc aggcatgatc cgcacgccgc gctcgttcgc
     1321 gtagcggcaa agctcctgcg ccgcctcgat cccgccatgc gaatcgcgca ggaagccata
     1381 gatcgcgatc gccgcgattc ggttcatgct catgaaatcg acgacccgct tgaaatcggc
     1441 caggaaccct tccggcggct tgtggtacgg gttcatgaca ccgatctcct gcacgccgat
     1501 ctgggtcatc tcccagttgg tggtgtggtc ccagttccag aaggtgcggt agggcaggcc
     1561 cggtgcctgc tcaacggtcc cgacctgaac ggcaccctcg gtcaactgcc gctgcaccag
     1621 ctcctcgacg ccgtagatca cgcccgaaaa cggcccgccc tcgacttcga cgaccggagc
     1681 ctcgccaccg ctcacgtgga tcgcaaatga cccctccgcc cgctcagtag ccgccaccaa
     1741 ccgaacagtg gtcgccccat cgggggccga cccgccgcca atcaccgtcc ccggcaacga
     1801 agcccgcaat cgccgcaaag catgcggatg ctccccacca ccctcaacaa ccaggtttcc
     1861 atcaagccga acaagctccg ccatgccgcg caagacaccc ccgaaaaacg aaccccgaaa
     1921 accgagaatc caaaacccat gacctaattg tcgcgcaccc aaccaaaccc ggtcgcctac
     1981 cgtagcccgg ttacatcacg aatttgtcac cgggtgtatg gtgttgggtg gtcgttttga
     2041 gcgaccggaa aggacggtag ttatggctac ccacattgac cgtcgcgaag atgtcagccc
     2101 gaaagaaggg ctccacgaat acggcgatgt caaattcgcc gatgaaacga acaagaagta
     2161 tccgatcgac accaaggagc acattcgtgc cgcctggagt tacatcaatc acaaggacaa
     2221 tgccgccaag tacgacaagg acgaagtgaa ggccatcaag ggccggatca agcgggcggc
     2281 catcgacaaa ggcgtcgata tcgagtccga ttaatacgca ttcaaacacc cgtgccggac
     2341 cggatcagcc acccggtccg accagccacc ttcggccccg ccccagccag cggggccgaa
     2401 ttcttgctcc atgcaaggtc accattgcgt gggcttcgcc tcgtatcatg tgcgccgatc
     2461 agtgagatga agcaaggatt gcgctcggag agatttggat ggaattgctc gatcgcctcc
     2521 tgggtcatga tcagtggacg acagagcgat gcctggagct ctgtcgagat ctgaacgagg
     2581 atcagcttga tcagccgttc gacatcggtc accgcacact gcgggcaaca tttgcgcaca
     2641 tgatctacaa cgtcgggttc tggacgagcc tgatgaccgg cgatacaccc gccgccaacc
     2701 cagagtcgcc ctcactggcc gagatgacgg aggtgcacga acgtacctac ctggtcttcg
     2761 cggacctcgc gaaccggctt tgcggcgacg gccgcctgga cgaaaccttc accgaccact
     2821 acgggatcac ccagacgttc ggcgcgacga tcatccaggt catcctgcac aatcaccagc
     2881 accggagcga tgtgctccac atcctccagc gcctcggcgt gcccgatctg cccgacggcg
     2941 atccccagga atgggagcac ctcaccggcc gcaccccctg gcgatcctga acggctcctg
     3001 cctgccaaga gttgtagacg tagcgcgggg ttagcacgcc ccgtctggtc cgcaatcggc
     3061 atctccgata tgaagactac gcccgttcac ggcgtagatt tagtcaaccg cccgcagggt
     3121 ggggccaagc ggggtgtggt aaccccgcgc tactgctcct ggacagtgac atgtgcgggg
     3181 ccggatttat gcggaccagg accgggccag atcggcgtat atctcgcggc aggtctggac
     3241 tgagtccaga tcgatccact cattgtccgc gtgcgcgccg tgaccagtcg ggccgaagac
     3301 gaggctcggg attccggccg cggacgtgag agcggagtcc atccagccgc cccagccgac
     3361 gatctccgtc ggctggccga gtcgctgagt gacgactgcc tgcatcgcct ggaccagctc
     3421 ggcctccggc ccgatctcga acggctggcg gctgatgccc atggtcaggt cggccgcgtc
     3481 gccgatcagc tcgcggagct gctgctcgac catcgcgtcc atctcgccgg gcaccgtccg
     3541 ccgctcgatc tggacccggc agcgatccgg gtaggtcg
//
LOCUS       DATAOZ010000226         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1676046, whole genome shotgun sequence.
ACCESSION   DATAOZ010000226 DATAOZ010000000
VERSION     DATAOZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676046"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1901)
                     /locus_tag="VHR64_05975"
     CDS             complement(<1..1901)
                     /locus_tag="VHR64_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3302412.1"
                     /translation="MAHLDDKTIIEKTHNTARYFIEQRQVAWVALIGTVLWGIIGYFS
                     MPQRKDPDIPAGVALVLCSWPGMDAERIEERVTRKIEEVVAENAQVNAVRSTTRTGIS
                     FVYVELKEDAKNTDAIFDDIALKLPAINDLPDGAGPIQFIRDFGDTAALMLTVASPRL
                     DEVQVSLRADQLRAAIQQLRAGARAGARATLVYNFPPSISAASAIRPARLYLEQAVHD
                     GVFRDARLLVGPQFVGVDGVTDVDDAGLIAHVSRFVEERVRVSEFHPDAWRAVVIRDP
                     SEIRAKLLAVAGDKYSYRELERFTDVMKRTFQTVKQVSKVDRAGILDEQVTLSFSQER
                     LASYGVPMGRLRDILKARNTALSGGLIEVSGRTVGLNPSGEFRNEREIGDVIVGTSKS
                     GSPLYLRDLVDIDRTYQSPPRYLNFYNWVDSTGHWHRSRPITMALQMRPGEKIGGFGY
                     AVDSTINVLRGRLPPDLIFARTSDQPLQVRENISLFMNSLFEAVVLVVVIALVGFWEW
                     RSAALLAVSIPLTLAMTFGMMSLLGIDLQQISIASLIIALGLLVDDPVVAGDAIKRDL
                     DHGHPPVVAAWLGPTKLAHAIMFATVTNIVAYLPHGSRVTGPAARASLPGALVHRDIK
                     PANIHVGRLG"
     gene            complement(1912..>2244)
                     /locus_tag="VHR64_05980"
     CDS             complement(1912..>2244)
                     /locus_tag="VHR64_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302413.1"
                     /translation="RVFDVEAALPNPDGRMKVGMLATLRIGSGGEAALFVPLAAIIRP
                     AGDSSGYAVYIVRDTAGAPVARLRRVSLGDVSGNMIGVRQGLAPGDRVIVRGATIVAD
                     GQPVRVMP"
BASE COUNT          418 a          738 c          694 g          394 t
ORIGIN      
        1 ccaagccgcc ccacatggat attcgcgggc ttgatatccc gatgcaccag cgcgccgggg
       61 agactggccc ttgctgctgg accagtgact cgagatccat gcggcagata tgcgacgatg
      121 ttggtgaccg tagcgaacat gatcgcgtgg gccagcttgg tgggtcccag ccaggccgcc
      181 acaacaggtg gatgcccatg gtcgagatcg cgcttgatcg catcgccagc cacgaccgga
      241 tcgtccacca ggaggccaag cgcgattatg agcgaggcga tcgatatctg ctgaagatca
      301 atgcccagca atgacatcat gccgaacgtc atcgccaggg tgagcggaat gctcaccgcc
      361 agaagagccg cagaccgcca ctcccagaac cccaccagcg cgatcacgac caccaggacc
      421 accgcctcga acaggctgtt catgaacagg ctgatgtttt cccgcacctg gagcggttga
      481 tccgaggtcc gggcgaagat cagatccggc ggcagtcgtc cccgcagcac gttgatcgtc
      541 gaatcgacgg cataaccgaa cccgccgatc ttctctcccg ggcgcatctg caacgccatc
      601 gtgatcggcc ggctccggtg ccagtggccg gtggaatcga cccagttgta aaagttgaga
      661 tagcgtggcg ggctctggta cgtgcggtcg atgtccacca gatctcgcag atagagcggc
      721 gagccactct tggaggttcc gacgatcacg tcaccgattt cccgctcgtt gcgaaactcc
      781 ccgctcggat tcagcccgac ggtccgcccg ctcacctcta tcaatccgcc ggaaagggcc
      841 gtgttgcggg ccttgaggat gtcacgcagc cgccccatcg gcacgccata cgaggcaagg
      901 cgctcctgcg agaagctcag cgtgacctgt tcgtccagga ttcccgcgcg atccaccttg
      961 ctcacctgct tgaccgtctg gaaggtccgc ttcatgacat cggtgaagcg ctcgagctcg
     1021 cggtagctgt acttgtcccc cgcaacggcg aggagcttgg cccggatttc cgaaggatcg
     1081 cggattacaa ccgcgcgcca ggcatcggga tggaattcgg agacgcggac ccgctcctcc
     1141 acaaagcgcg agacgtgtgc gatcaggccg gcgtcgtcta cgtcggtcac cccatccacg
     1201 ccgacgaact gcggcccgac cagcaggcgg gcgtcgcgaa acacgccgtc gtggactgcc
     1261 tgttccaggt agagcctcgc cgggcgaatg gcgctagccg cggaaatcga aggcgggaaa
     1321 ttgtacacca gggtcgcgcg cgcgccggcg cgggctcctg cccgcaactg ctggatagct
     1381 gctcgtagtt ggtcggcgcg cagagagacc tgaacctcgt cgagtcgcgg gctggccacg
     1441 gtcagcatca gcgccgccgt atcgccgaaa tccctgatga actggatcgg acccgctccg
     1501 tccggcaggt cgtttatcgc cgggagcttg agagcgatgt cgtcgaaaat cgcgtcggtg
     1561 ttcttggcgt cttccttgag ctcgacgtag acgaagctga tgccggtgcg cgtggtcgag
     1621 cggacggcat tgacctgagc attctccgcc acgacctctt cgatcttgcg ggtaactcgc
     1681 tcttcgatcc gctcggcatc catgcccggc caggagcaga ggacaagcgc aacgccggcc
     1741 gggatatcgg ggtccttgcg ctgcggcatg ctgaagtagc cgatgatgcc ccacaggaca
     1801 gttcctatca gggcaaccca cgcgacctgc cgctgctcga tgaagtaccg ggccgtgttg
     1861 tgggttttct cgatgatcgt tttgtcgtcc agatgagcca cagggactcc tctacggcat
     1921 gactcgcacg ggctgtccgt cagccacgat cgtcgctccg cggacgatta cccggtcgcc
     1981 aggtgccaag ccttggcgca cgccaatcat gttgccgctc acgtcgccca ggctcacgcg
     2041 gcgcagtcgg gccacgggcg cgcctgcagt gtcccgcact atgtacacgg catagccgga
     2101 ggaatcgcct gccggccgga tgatggccgc caacggtacg aacagggctg cctcacctcc
     2161 actcccgatg cgcagagtgg cgagcatccc gaccttcatc cggccatcgg gattgggcag
     2221 cgcagcctcg acgtcgaaga ctcg
//
LOCUS       DATAOZ010000227         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9302119, whole genome shotgun sequence.
ACCESSION   DATAOZ010000227 DATAOZ010000000
VERSION     DATAOZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9302119"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1658
                     /gene="ilvB"
                     /locus_tag="VHR64_05985"
     CDS             15..1658
                     /gene="ilvB"
                     /locus_tag="VHR64_05985"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX3302414.1"
                     /translation="MPAYDAMLDYPVRHVLVRHEQGAAHMADGYARASGEVGVAIATS
                     GPGATNLVTGIATAQMDSSPLVCITGQVPSKLIGYDAFQETDISGITLPITKHSYLVT
                     RVDEIMPTIKEAFYLAKSGRPGPVLVDITKDAQQATTDWTWNEAPVKLRGYQAHRIPA
                     KSDMTKALELIKAARKPLILAGQGVIQSNAMKELRAFAEMIDAPVTVTLLGIGGFAAS
                     HPLSIGMMGMHGEAWVNHAIQEADLLLALGMRFDDRVTGNLKTYAPHAKKIHIDIDPS
                     EINKNVRAEAAIVADLREALRELLDMLEPSSHGPWLKYIASMKGDTAVRDIQRLPDNG
                     HLYAAHVINDIWRLTNGNAIIATDVGQHQMWEAQYYHHNDPRTLITSGGLGTMGFGLP
                     AAIGAKLAKPDAEVWAIVGDGGFQMTQPELSTAAQEGVKVNVAIINNGYLGMVRQWQE
                     FFYERRYAATPLRSPDFVKLAEAHGLMGLRAEKRSEVDAIVDRARQAEGTVVIDFRVE
                     QEDSVYPMVPSGADLDDMIRRPPSGSEAAGTSPWTTGAV"
BASE COUNT          349 a          540 c          548 g          290 t
ORIGIN      
        1 cgggcggagc gattctgccc gcctacgacg cgatgctcga ttacccggtc cggcacgtcc
       61 tcgtgagaca tgaacagggc gcggcccaca tggctgacgg ctatgcgcgc gcaagcggag
      121 aagtcggggt tgcaattgcg acttcggggc ccggagccac caatctcgtt actggcattg
      181 caactgcgca gatggattcg tcgccgctcg tctgcattac agggcaagtg ccctcgaagc
      241 taatcggcta tgacgcgttt caggagactg atatcagcgg catcacgcta ccgattacca
      301 agcacagtta tctcgtcacg cgcgtcgacg agattatgcc gaccatcaag gaagcgttct
      361 atctggccaa gtcgggccgt ccgggaccgg tgctggtgga tatcaccaaa gacgcgcagc
      421 aggcgaccac tgactggacg tggaacgagg cgccggtgaa gctgcgtggc taccaggcac
      481 accgcattcc tgccaagtcc gacatgacca aagccctcga gctaatcaaa gcggcacgca
      541 agccgctgat tctcgccggc cagggtgtca ttcagagcaa cgcgatgaag gagctgcgcg
      601 cgttcgccga aatgatcgat gcgccagtta cggtgacgtt gctcgggatc ggcggcttcg
      661 cggcgagcca tccgctcagc atcggcatga tggggatgca cggcgaagcg tgggtgaatc
      721 acgccattca ggaggcggac ctcctgctgg ccctgggcat gcggttcgac gatcgcgtga
      781 cgggcaatct gaaaacctac gctccgcacg ccaagaaaat ccacatcgac atcgacccgt
      841 cggagatcaa caagaacgtc cgggccgaag cggcgatcgt ggcggacctg cgcgaagcgc
      901 ttcgcgagct gctcgacatg ctcgagccgt cgagccacgg cccttggctg aaatacatcg
      961 cgtcgatgaa gggcgatacc gcggtccgcg acatccagcg gctcccggac aacggccacc
     1021 tctatgcggc gcacgtcatc aacgacatct ggcggctgac aaacggcaac gccattattg
     1081 cgaccgatgt gggccagcac cagatgtggg aggcgcagta ctaccaccac aatgatcccc
     1141 gcacgctcat cacatcaggt ggtctcggca cgatgggatt tggcctgccc gcggcgatcg
     1201 gcgccaagct ggcgaagcct gacgcagagg tgtgggcgat cgttggcgac ggcggcttcc
     1261 agatgacgca gcccgagctg agcaccgcgg cgcaggaagg cgtgaaagtg aacgtcgcga
     1321 tcatcaacaa tgggtatctc gggatggtgc ggcaatggca ggaattcttt tacgagcgcc
     1381 gctacgccgc gacgccgctc cgtagccccg atttcgtgaa gcttgcggaa gcgcacggtc
     1441 tgatgggact tcgcgctgag aaacgctctg aggtggatgc catcgtcgat cgtgcacgcc
     1501 aggccgaagg aactgtggtt atcgacttcc gcgtggagca ggaggacagc gtctacccca
     1561 tggtcccgtc gggcgcggat ctcgacgaca tgatccgccg tcctccatcg ggcagcgagg
     1621 ccgctgggac gtcgccgtgg accaccggtg ccgtctgagg agccccgcca tgagttcctc
     1681 tcgcccccgc accttcatcg cccacgtcga ggaccgtccc ggagtcc
//
LOCUS       DATAOZ010000228         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_622221, whole genome shotgun sequence.
ACCESSION   DATAOZ010000228 DATAOZ010000000
VERSION     DATAOZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622221"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VHR64_05990"
     CDS             complement(<1..417)
                     /locus_tag="VHR64_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HEX3302415.1"
                     /translation="MSDSLQTFDVGKIDALVAETVGQDDKVDVPEEIQTQMAGDMEEP
                     EHDLERDLDVLQRLDPNFINDPVRLYLREIAETPLLTHQQEIDLAKRVEMDDQEATQL
                     FVRANLRLVVSIAKRYVNRGLTLLDLIQEGNIGLMRA"
     gene            complement(905..1285)
                     /gene="erpA"
                     /locus_tag="VHR64_05995"
     CDS             complement(905..1285)
                     /gene="erpA"
                     /locus_tag="VHR64_05995"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX3302416.1"
                     /translation="MAATSTPMLSITDEAVNQLKSFLDEQGTPDHALRVFVAPGGCSG
                     LQYGMTIEEAADEGDEVIETDGVRIIVDNFSAMYLGGATVDFVNSLMGGGFTIHNPNA
                     VASCSCGHSFDTGGDGQTAHGCSR"
     gene            complement(1412..2260)
                     /locus_tag="VHR64_06000"
     CDS             complement(1412..2260)
                     /locus_tag="VHR64_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX3302417.1"
                     /translation="MAMPKATVCLTFDFDALSVWIGPRGATSPSATSRGEYAVLGVER
                     VLKVLSERGILATFFIPGHTVETYPRSIEAILAAGHEIGHHGYLHENPCALPSKDEER
                     RVIERGLKALDEVARLKPVGYRSPSWDNSPYTTDLLLEYGFRYESSLMADDFTPHWCR
                     VGDIPNTDSAYVFGQPVDLVEMPVSWVLDDFPHFEFLRYPNVVFPGLSAPSKVEEIWK
                     DEFSYMYRDVPNGIYTLTMHPEIIGKGHRILMLERLIDWMADHDGVSFTRLADAAEQW
                     RGEQSV"
     gene            complement(2341..3222)
                     /locus_tag="VHR64_06005"
     CDS             complement(2341..3222)
                     /locus_tag="VHR64_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302418.1"
                     /translation="MQLAFAQIDRSSALDERITELLATSATHLPQDPLRSLYFANEAL
                     VLAETGDRIELELRALRQIVEALRSAGRQADATPYIVRAIDLADSIHDTDLLAALVDA
                     LGTWAIAVEQQPVESDPRRTRTSRDWAVAAISRLERQFPNNAPESAPMREATIDDPDT
                     GLLNSLGMAAELLTLEEQATSYALIQIVLTGHTLPLLTSVARSAAQLVGDRGLVARNG
                     LAMLTAILPSFTGIASMSLAEQMRAAFVKQSADSFARIGIGVAIKQPGESSRDVLRRV
                     ADRAEEATLAAGVTVCG"
     gene            complement(3386..3844)
                     /locus_tag="VHR64_06010"
     CDS             complement(3386..3844)
                     /locus_tag="VHR64_06010"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX3302419.1"
                     /translation="MEQPVRSPAVRKRDTLHRLEHDVDGWVATADSAGGTPYLVPLSF
                     LWDDQTLVISTPTASPTSRNLLASGRVRIGIGPTRDLILIEGTARPLSKDEVTDELGD
                     AFAVKTGFDPRELTTPYTYFRIQPVRLQAWREANELDGRDLMRDGRWLMR"
     gene            complement(3863..>4237)
                     /gene="tsaA"
                     /locus_tag="VHR64_06015"
     CDS             complement(3863..>4237)
                     /gene="tsaA"
                     /locus_tag="VHR64_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX3302420.1"
                     /translation="ESPLIDRSAAPKQGDEGAPVARIIFEPQYFEAARDLRPGDEVLV
                     LTWLHLGERATLAVHPRGDLSRPITGVFSTRSPDRPNPIGLHAVTIENVADGAISVRN
                     LEAIHGTPVLDIKPVLADQRER"
BASE COUNT          778 a         1290 c         1361 g          808 t
ORIGIN      
        1 ggcgcgcatc aggccgatat tgccttcctg gatcaagtcc aggagggtca gaccgcgatt
       61 cacatagcgc ttggcgatcg agaccaccag acggaggttt gcccggacga agagctgcgt
      121 tgcttcctgg tcgtccatct ccacccgctt ggcgagatcg atctcctgct gatgggtcag
      181 caggggggtt tcagcgatct cgcgcaggta gaggcggacg ggatcattga tgaagttcgg
      241 gtccaggcgc tgaaggacat ccaggtcacg ctcgagatcg tgttccggct cctccatgtc
      301 gccggccatc tgcgtctgga tttcttcggg cacatcgacc ttgtcgtcct ggccgacggt
      361 ttcggcaacc agggcgtcga tcttcccaac gtcaaacgtc tgcaacgaat cactcattaa
      421 gcgcattgct ccttacagat cggtccgtct cgaaacaatg tctgcggaca gtacaccatt
      481 cttggaagcg acgcgatgcg caaagaaccg ggcgaccaaa tcagttgcgt gattcgcaac
      541 acgtgaaggt acgtagccct ggtcggcggg attctttgct ctgcggttct ctggtcgaat
      601 gatgcgactg ctcgcggtac ggtcctacgg gcgagacaga gtgagaggcg ttgtgagtca
      661 acaagccgcc ccacaacttg tgaatattat cacagattaa gggatttggg aagagggtaa
      721 gttgtagtga ctgcaacgca cccacctttg cgcacgccta tggaaccgct cccacagccc
      781 atcatatcgg ctcccgacaa aagtgcaacc cctcggttcc gatgcgggct tttccggaga
      841 caattaacgg cgcggtccga gtggaccgcg ccgcatttgg gtgcgcttgc gattattgaa
      901 cgccctagcg gctgcagccg tgggcagtct ggccgtcgcc gccggtatcg aacgaatgac
      961 cgcacgagca ggaggcaacc gcgttcgggt tgtggatcgt gaagccgcca cccatgagcg
     1021 agttgacgaa atcgaccgtc gcgccaccca ggtacatcgc gctgaagttg tcgacgatga
     1081 tccggacgcc gtcggtctcg atcacctcgt cgccctcgtc ggccgcttcc tcgatagtca
     1141 tgccgtactg gaggcccgag cagccgcccg gcgccacgaa gacgcgcagc gcgtgatccg
     1201 gcgtgccttg ctcatccagg aacgacttga gctggttcac ggcctcatct gtgattgaaa
     1261 gcatcggtgt gcttgtcgcg gccacccggt actccttatg tatcaacctt acggttcgtt
     1321 gaacgatttg acagtccgac caaatcagtc aggctttact tacgttagga tagtttaacc
     1381 catccgcctg tcaaggtcgc gttgagttcc ttcacacgga ttgctcacca cgccattgct
     1441 ccgcggcgtc ggcgaggcgg gtgaagctca caccgtcgtg atcggccatc cagtcgatta
     1501 gccgctccag catcaggatg cggtgcccct tgccgatgat ctccgggtgc atggtcaggg
     1561 tgtagatgcc gttcggcacg tcccggtaca tgtaggagaa ctcgtccttc cagatctcct
     1621 cgaccttgct cggcgcggaa agacccggga acacgacgtt cgggtagcgc aggaactcga
     1681 agtgcgggaa gtcgtcgagg acccagctga ccggcatttc aaccagatcg accggctggc
     1741 cgaagacata ggctgaatcg gtgttcggaa tatcgccgac gcggcaccag tgcggcgtga
     1801 agtcgtccgc catcagcgag ctctcgtacc ggaagccgta ttcgagcaac aggtcggttg
     1861 tgtagggcga gttgtcccac gatggcgacc ggtagccgac cggtttcagg cgcgcgacct
     1921 cgtccagcgc cttgagtccg cgctcgatca cccgccgctc ctcgtccttc gatggtaggg
     1981 cgcaggggtt ctcgtgcagg tagccgtggt gtccgatctc gtgacccgcg gcgaggatcg
     2041 cctcgatcga acgcgggtac gtctcgaccg tatggccagg gatgaagaag gtggccagga
     2101 tgccacgctc ggacaggacc ttgaggaccc gctcgacacc gagcaccgcg tactcgccgc
     2161 gagaggtcgc gctcggcgag gtcgcccctc gcggcccgat ccagaccgag agcgcgtcga
     2221 aatcaaacgt cagacaaacg gtggctttgg gcatcgccat gcgaatccgc tccccatcaa
     2281 tgtcggaacc gaccaatggg ctggattctc gcacggatcc ggagtgtgga cgtcgatcgc
     2341 ctatccgcag acggtcacgc cggcggcgag ggtcgcttcc tcggctcgat cggcgacgcg
     2401 gcgaagcacg tcacggctgg attcgccggg ttgcttgatc gcgacgccga taccgatccg
     2461 ggcgaaggag tctgctgact gctttacgaa cgcggcccgc atctgttcgg cgagcgacat
     2521 ggaggcgatg ccggtgaacg agggcaggat ggccgtcagc attgccagtc cgttccgcgc
     2581 gacaaggccc cggtcgccga cgagctgggc cgcactgcgc gcgactgagg tcagcaatgg
     2641 cagcgtatgg ccggtcagga cgatctggat cagcgcatag ctagtggcct gttcctcgag
     2701 cgtgagcagt tcggccgcca tcccaaggga gttgagcagc ccggtgtccg gatcatcgat
     2761 cgtcgcttcc cgcattggtg cggattccgg cgcgttgttc ggaaattggc gttccagcct
     2821 ggagattgcg gcgactgccc agtcgcgcga cgtccgcgtc cgtctcgggt ccgattcgac
     2881 aggctgttgc tcgaccgcga tcgcccacgt gccgagcgca tcgaccagcg ccgcgaggag
     2941 gtccgtatcg tggatcgagt cggcgagatc gattgcccgg acgatgtacg gcgtggcatc
     3001 ggcttgccga ccggcggagc gcagtgcttc gacgatctgc cgaagggcgc gcagctccag
     3061 ctcgatgcga tcgccggttt cggccagcac cagagcctca ttggcaaagt agagcgaacg
     3121 gagcggatcc tgggggaggt gagtcgcgga ggttgccagc aattccgtga tccgctcgtc
     3181 gagggccgac gaacggtcga tctgcgcgaa cgcgagttgc aacggttgct ccaccagcca
     3241 gcctgacgca taccggatag gaaaacgagc acgctcgttt atcgtcaatt ctatgcaaag
     3301 gtgtgcattt gcataatgtt tcggctagga ctggagtcat gattggattc ggcaacctgg
     3361 tagtcaggcg ttgcgctttc cgcaatcagc gcatgagcca gcgaccatcc cgcatgaggt
     3421 cgcggccatc cagctcgttc gcctcacgcc aagcctggag acgcaccggc tgaatccgga
     3481 agtaggtgta gggcgtggtg agctcgcgcg gatcgaaccc ggttttgacg gcgaaggcat
     3541 cgccgagctc gtccgtgacc tcgtccttcg agagtggccg cgcggttccc tcgatcagga
     3601 tcaggtcgcg ggtcgggccg atcccgatgc ggacccggcc cgaagccagc agattccggc
     3661 tcgtcgggct agcggtcggg gtcgaaatca ccagggtctg gtcatcccag agaaaggaga
     3721 gcggcaccag gtacggagtc ccgccggcgc tatcggccgt cgcgacccag ccatcaacat
     3781 cgtgctcgag ccggtgcagc gtgtcccgtt tgcgcaccgc tggtgatcgg accggttgct
     3841 ccatcgacgc actacctcca gatcatcgct cgcgctggtc ggcgagcacg ggcttgatgt
     3901 cgagcaccgg cgtgccgtga atcgcctcga ggttgcggac cgagattgct ccgtccgcaa
     3961 cgttctcgat cgtgacggca tgcaggccga tcgggttcgg gcgatccggc gagcgtgtcg
     4021 agaagacgcc ggtgattggc cggctcaggt cgccgcgtgg gtggacggcg agcgtcgcgc
     4081 gttcacccaa gtgcaaccag gtcagcacca gcacctcgtc gccagggcgg agatcgcgcg
     4141 cggcttcgaa atactgcggc tcgaatatga tgcgcgcgac cggagctcct tcatcaccct
     4201 gtttgggagc ggcggagcga tcaatcagcg gcgattc
//
LOCUS       DATAOZ010000229         7364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7727392, whole genome shotgun sequence.
ACCESSION   DATAOZ010000229 DATAOZ010000000
VERSION     DATAOZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7727392"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VHR64_06020"
     CDS             <1..467
                     /locus_tag="VHR64_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302421.1"
                     /translation="DELVEKVRAIAEGAALITGARLEWVHPSPANADMVTNFTLARTL
                     DKHVREVGLAMPEAKAEQASGSTDWGNVSYVVPSVETSYPIIDRVCTWHSMDVVNAAD
                     SEMGYENTIRVAKAMALAGLDVMRDEELRMAVRAEFEQKLPVETREMVRSLA"
     gene            complement(1181..2932)
                     /locus_tag="VHR64_06025"
     CDS             complement(1181..2932)
                     /locus_tag="VHR64_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3302422.1"
                     /translation="MSEDFFTDRYLIERVVLERDFVTRSVAVDRLLGREVLITHLTGQ
                     VGRRAAVQERFRDAARKAVRLSHVNIVALYDIGTANGFPYSVQEHTHSEVLTEIIAHE
                     GPFHPDDVIVLVEQVAAALDYAALRDLYHLALSPAAITVDYGGQVLVSDFGIGRVLND
                     ISPTDVAKLRYRAPEQMTGAEGDARSDVFSLGVIAYEMLTGRPPFDITSVEALRTSVA
                     EAKPKPLSAIDPNLSARLSSIVIKSLADDPDHRFQSAGHFADALLDWSDPDRTTGPVY
                     ETSPIRSVDATLPIQIAPEPEPESADLAPFTEEEHGQWPRGTAVAAWLTVAIALVALT
                     WIGMRLLRDDNSSGNQAAEITPTALSTSTVQAVPTAISLVGMTVDQASASTNFRIRVV
                     GTETSDTVPAGQIIRQSPNAGNPVRTGELIVVVSDGPTAQPIQLSDIQVQGTPFDQVA
                     QQLTGQGLNVSQVQEGSQSVPEGQVIRIDEQSANPGDTVHVVVSMGDMVQIPADLQSK
                     PLDEAVSQLKDLGLNVGEPIAVSKSRIESFQVNLDELGIVDGDVVGIQEEGAGFGAWV
                     PRGSTVTPVYYDASLTS"
     gene            complement(2925..3809)
                     /gene="ispE"
                     /locus_tag="VHR64_06030"
     CDS             complement(2925..3809)
                     /gene="ispE"
                     /locus_tag="VHR64_06030"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX3302423.1"
                     /translation="MIRILTPAKLNLGLEILSRRDDGYHEIRTIFCAVSLFDKVTVTT
                     SSADQVACLPAVDTPENLVSVALRNAREVTPELPGICVTVEKRIPAAAGLGGASSDAA
                     ATLLAISRLHPDQFRECSIAELAAQCGSDVPFFLHSGLALGEGRGEQLRRLSFVPIHA
                     VIITPLVDIPDKTRAMYARIRPTDWTDGGQVASIAEQLGRGEVVGTGERLPNAFRQPL
                     YDLFPDIRALAEEIESMLGRSVQVTGAGPSLFVLCSGSADALSVRRELSARVDPDQAH
                     LHTVRSVSRVLITGNPDV"
     gene            complement(3806..4666)
                     /gene="rsmA"
                     /locus_tag="VHR64_06035"
     CDS             complement(3806..4666)
                     /gene="rsmA"
                     /locus_tag="VHR64_06035"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX3302424.1"
                     /translation="MTGSPLPTKKRDWQRLIDDLGLRPSKGRGQNFLHDPAVIHRIVK
                     EAQVTADDHVLEIGPGLGILTRDLLERAGRVSAIELDPTLAQHLEMTFGSRPTFALHT
                     GDALKADLGDIAAGEPIKVVANLPYSAAAAIVQHILESDVALVSATLMVQREVAERML
                     AEPPDMSILSVATQLHATGRIAFIVPPDVFLPAPKIESAVITLVPRESPLIQPQDRPQ
                     FFRLVNGGFRHKRKNIANSLADETRHGKGEIAAVLESASIDPGRRAQTLNIPEWLALN
                     QVWPWKDIAP"
     gene            complement(4663..5559)
                     /locus_tag="VHR64_06040"
     CDS             complement(4663..5559)
                     /locus_tag="VHR64_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX3302425.1"
                     /translation="MTNPEIAVIVNPRAAGGKALKTLPVINSTLESLNCPYAIYVTKG
                     RGDAIQAARSYAEGGASRILAVGGDGTVNEVANGMYQSSADAALGLVPVGHGTDLARY
                     LGTTRDVARAVKRACEGDMSRIDLGLARYADGTERAFINIAGLGFDAIVASKAQKSRL
                     PGGNLPYLGSALTTLIGFQNLQVKIDADGQLIETPGVFVQIANAQYMGGGYHFAPMAS
                     MDDGLLDVCIVGDFAKLELIRAIPGVYKGKHVTNPKFSHVAAKCISIETSTPAMVQLD
                     GELVGQTPVQFSVLPAAIALVK"
     gene            5738..6085
                     /locus_tag="VHR64_06045"
     CDS             5738..6085
                     /locus_tag="VHR64_06045"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEX3302426.1"
                     /translation="MTVTFHLTPRERWDAQRATAAYTPEPFESEGFIHCTDGEANVVA
                     VGNRYYTGDPRGIVCLVIDCELVNQEIRYEDPERIYPHIFGPLNTNAVIDVRSVRREA
                     DGSFTGIGESVSE"
     gene            6119..>7364
                     /locus_tag="VHR64_06050"
     CDS             6119..>7364
                     /locus_tag="VHR64_06050"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3302427.1"
                     /translation="MTDTRQPSPGRSIFRVLGAAMYRHRFLVVSLWAIAFLISIPMLL
                     RVEEPLKVGGFSSDRTEAARARLTVERELSGSASQLVVIFKSTDQPIATPDEQQRIAA
                     AIEPFRGHAHVIDVVLPSENGAQISKDGRTAYALVSLDLPAEEAQRLIPDFERLIVPQ
                     PGLQVLLAGGPAFYADIETVSQRDLQRAELIAFPFALVALLFVFGTAIAALLPLIVGG
                     LGVAAVLMTIFGLAHVTDLSIFVLNLATMLGLGLAIDYSLFITSRFREELPRSESVER
                     AVERTMVTAGRAIFFSGLTVLIGLSGLIMFDFMFLRSVGIAGVVVVFFSVLAALTLLP
                     ALLSIAGSNVERWAVIRRSPDDHHRHGFWARLSREVMKRPVLILIPTAILLLAFGAPF
                     RHVNISSPDATILPKSTDSRKGF"
BASE COUNT         1336 a         2231 c         2354 g         1443 t
ORIGIN      
        1 tggatgagct ggtcgagaag gtccgggcga ttgccgaggg tgcggcgctg atcacgggcg
       61 cacggctcga gtgggttcat ccgagcccgg ccaacgccga catggtcacg aacttcacgc
      121 tcgctcgaac actcgacaag cacgttcgcg aggtcggact tgcgatgccc gaggcgaagg
      181 cggagcaggc gtccggctcg accgattggg gcaacgtcag ctacgtcgtg ccgtcggttg
      241 agacctcgta cccaatcatc gatcgcgttt gcacctggca ttcgatggac gtggtgaacg
      301 cggccgattc agagatgggc tatgagaaca cgatccgcgt tgccaaagcg atggccctgg
      361 ctggactgga cgttatgcgc gacgaggagc tccgcatggc ggtccgggcc gagttcgagc
      421 agaaactccc ggtcgagacg cgcgagatgg tccgctcgct cgcctgacac gttcgccgca
      481 aagaacgacc gagggatccg gaacgtcgag ccatcgattc ggatcgacag gactgttccg
      541 gctcggacag tcgcaatcat ctgagttcgg gccagcccat tcgaggctgg cccgattcgc
      601 acctgggggt ctgattccga agcgccgaat gcgccgtcga gcgagatcca taccggcgct
      661 gtactcgccg atctccccgg tgttatcgct acggcgccag ccggcgtcgt cacttcggcc
      721 gtccaggagt tgtcgggctc aattcgaacc gcgagcgcga cgcctggtgt caattcgatc
      781 gtaaacgacc gctgcacggt ctcagctccg ttctcagagc cggcttcggg cagcacaaga
      841 gcgcgcttgg catcgagaga tcgcgcacgg tcgacaacat cggcagagga gcgtgggtcg
      901 atcaggagta catcgatctc ctcgccgatg gccggcaatg cgttgcttat tgcgttcgag
      961 aaggctgagc cgttgctgcc ggacgcaatc aggattcgtc gctggtcctg cgtgatcagg
     1021 acggatgcgt atttgccggc gccgaggacc tgaatccggg taccggcgcc ggcttcggtc
     1081 catcgccagg caagaaccag cgcgaggacc aggccgacga ctaatccggc acagagtgaa
     1141 agccgaacca gcgccggatc atgcggatcg ggctggctgc tcacgacgtg agcgaggcgt
     1201 cgtagtaaac cggggtgacg gtcgagccac gcggaaccca tgcgccgaag ccggcgcctt
     1261 cctcctggat gccgaccacg tctccgtcga cgatgccgag ctcatcaaga ttcacctgga
     1321 acgactcgat acggctcttc gaaaccgcga tcggctcgcc gacattcaga ccgagatcct
     1381 tgagctgact cacggcctcg tctagcggct tcgattgcag gtcggccggg atctggacca
     1441 tgtcgcccat gctgacgacg acatgaaccg tgtcgcccgg gttcgcagac tgctcatcga
     1501 tccggattac ctggccctcg ggaacgcttt ggctgccttc ctgaacctgg ctgacattca
     1561 gcccctggcc ggtcaactgc tgggcgacct gatcgaacgg cgtgccctgg acctggatgt
     1621 cagagagttg aattggctgg gcagtggggc catcgctgac gaccacaatc agctcgccgg
     1681 tccggaccgg gtttcctgcg ttgggagact gccggatgat ctggcccgcc ggaacggtgt
     1741 cgctcgtctc cgtcccgacc acccggatcc ggaagttcgt gctggcgctg gcctgatcga
     1801 ccgtcatgcc gactagtgag atggccgtcg gaaccgcttg caccgttgac gtgctcaggg
     1861 cagttggcgt gatttctgcg gcctggttgc cggacgaatt gtcgtcccga agcaatcgca
     1921 taccgatcca ggtcagcgcc accagggcaa tcgcgacggt gagccatgcc gcgactgccg
     1981 tgccgcgcgg ccactggcca tgctcttctt cggtgaacgg ggccaggtcg gcggattccg
     2041 gttcaggctc cggtgcgatc tggatcggca gcgtagcgtc gaccgatcgg atcggcgatg
     2101 tctcgtagac cgggccggtt gttctgtctg gatcactcca atccagcagt gcgtccgcga
     2161 aatgacccgc ggactggaag cgatgatcag gatcatcggc gagtgacttg atgacgatgc
     2221 tggatagcct cgccgacagg tttggatcga tggctgaaag tggttttggc ttggcctcgg
     2281 cgacgcttgt cctgagcgct tcgaccgacg tgatatcgaa tggcggccgg ccggtcaaca
     2341 tctcataggc gatcacgccg agggagaaaa cgtctgagcg ggcatcgccc tcggctcccg
     2401 tcatttgctc gggagcgcga tatcgcaact tcgcgacgtc cgtcggcgag atgtcgttga
     2461 gaacgcggcc gatcccgaag tccgagacga gcacctggcc gccgtagtcg accgtgatcg
     2521 cagccggaga aagcgcgagg tgatagagat cgcgcagcgc agcgtaatcg agcgctgccg
     2581 cgacctgctc aaccagcacg atgacgtcgt cggggtggaa tggaccctcg tgggcgatga
     2641 tttccgtgag aacctcggaa tgcgtgtgct cctgaacgga gtaggggaag ccattcgccg
     2701 tgccgatgtc atagagggcg acgatgttaa cgtgcgagag acgtaccgct ttgcgggcgg
     2761 catcgcgaaa tcgttcctga acagcggcgc gccggccgac ctggccggtg aggtgcgtga
     2821 tcaggacttc gcgaccgagc aagcgatcca cggccaccga ccgcgtcacg aaatcgcgct
     2881 cgagcaccac gcgctcgatg aggtatcggt cggtgaagaa gtcctcagac atctggattc
     2941 ccggtaatca gcacgcggga tactgagcgc accgtgtgaa ggtgcgcctg gtccgggtcg
     3001 acccgagcgg acaactcgcg ccgaaccgaa agagcatccg ccgatccgga gcagaggaca
     3061 aaaagcgatg gccccgcacc ggtgacctgc acgctacgtc cgagcatcga ttcgatctct
     3121 tcggcaagcg ctcgaatatc cggaaacaga tcatacaggg gctgtcgaaa tgcgtttggt
     3181 aatcgctcac ccgtgccgac gacctcgcct ctgcccagtt gttcagcgat cgacgcgacc
     3241 tggccaccgt ccgtccagtc ggtgggccga atccgggcat acattgcccg ggtcttgtcc
     3301 ggaatgtcga cgagtggcgt gatgatcacc gcgtggattg gcacgaacga gagccggcgg
     3361 agttgctccc cgcgtccctc tccgagtgcg agaccggaat gcaggaagaa gggaacatcg
     3421 cttccgcatt gcgccgcgag ctcggcgata ctgcattcac gaaattgatc gggatggagt
     3481 cggctgatcg caaggagcgt cgccgccgca tcgctgctgg cccctcccaa tccagcggca
     3541 gccggaatcc gtttctcgac agtcacgcaa atgcctggca gctcgggtgt gacttcgcgc
     3601 gcatttcgca gcgccaccga gacgagattc tcgggcgtat cgactgcggg caggcacgcc
     3661 acctgatcag ctgatgaagt tgtaacggtt accttgtcga acagagacac cgcacagaag
     3721 atcgtccgga tttcgtggta gccgtcatcg cgacggctga ggatctcgag accgagattg
     3781 agcttggccg gcgtcaggat acggatcatg gcgcgatgtc tttccagggc cagacctggt
     3841 tgagagccag ccattccggg atgttcagcg tctgggcacg acgtccggga tcgatcgatg
     3901 ccgactccag cacggccgca atttcgccct tgccgtgccg cgtttcgtcg gccagtgaat
     3961 tggcgatgtt cttgcgcttg tgccggaagc cgccgttgac aaggcggaag aattgcggcc
     4021 gatcttgcgg ctgaattaac ggagattcgc gtggcacaag cgtaattacc gcggattcga
     4081 tcttgggagc cggcagaaat acgtcgggcg gaacaatgaa cgcgatccgg ccggtcgcat
     4141 ggagttgcgt cgcgaccgag aggatcgaca tgtcgggcgg ctcggccagc atccgctcgg
     4201 caacctctcg ctggaccatc aaggtcgcgg aaaccagcgc aacgtccgat tcaaggatgt
     4261 gctggacgat tgccgccgcg gccgaatagg gcaggttcgc gacaaccttg atcggctctc
     4321 cggcggcgat atcgccgagg tccgccttca gggcatcgcc cgtgtgaagg gcgaaggtcg
     4381 gccggctgcc gaatgtcatc tcaaggtgct gggcgagcgt cggatcgagc tcgatggccg
     4441 aaacccgacc ggcccgttcc agcaggtcgc gcgtcaggat cccaagaccc gggccgattt
     4501 cgagcacatg atcgtcggcg gtgacttgtg cctccttgac gatccggtgg atcaccgccg
     4561 ggtcatgcag aaaattctga cctcgcccct tgcttggccg gaggcccagg tcgtcgatca
     4621 gtcgctgcca gtcacgcttc ttcgttggga gtggagatcc ggtcatttca ccaatgcgat
     4681 ggccgccggg agaacactga actgcacggg agtctggccg accaattcgc catccaattg
     4741 aaccatggcg ggagtcgagg tctcgatcga aatgcacttc gcagcgacat ggctgaactt
     4801 cggattggtg acgtgcttgc ccttgtagac gcccgggatg gcgcggatca gctcgagctt
     4861 ggcgaaatct ccgacgatgc agacatcgag tagaccgtcg tccatgctcg ccatcggcgc
     4921 aaagtgatag ccaccaccca tgtactgcgc attcgcgatc tggacgaaga cgcccggtgt
     4981 ttcgatgagt tggccatccg cgtcgatctt gacctgcagg ttctggaagc cgatcaatgt
     5041 ggtcagcgcg ctgccgaggt aggggagatt cccgcccggg agcctcgatt tctgcgcctt
     5101 ggaggcaacg atcgcatcga agccgaggcc ggcaatgttg atgaacgccc gctccgtgcc
     5161 atcggcgtag cgcgccaagc ccaggtcaat tcgcgacatg tcgccttcac acgcgcgctt
     5221 gacggcccgc gcaacgtccc tggtcgtgcc gaggtagcga gcgagatcgg tcccgtggcc
     5281 cactggaacc aggcccagtg cagcatcggc gctggactgg tacatgccgt tcgcgacttc
     5341 gttgactgtg ccatcgccac cgaccgcaag gatgcgggac gcgccacctt cggcatagga
     5401 tctggccgct tgaatggcat cgccgcgccc tttggtgacg taaattgcgt acgggcaatt
     5461 cagggactcg agcgtactgt tgatgaccgg caaggtcttg agcgccttgc cgccggcagc
     5521 ccgcggattc acgatcacgg cgatttctgg gttggtcacg ttggcatcct ttgccggcga
     5581 tcgacaaccg cgcagacaac acggaatctg gacgaattat ttcatccatt cgaagagtac
     5641 ccgataccga aaatggcgcg catactgatc tgaagtatcg ttatggtcga atgtggcgat
     5701 ccgctcattc tctggcttga gccgcaagga tccaactgtg acggtcacct ttcatttgac
     5761 tccgcgcgag cgctgggatg cccagcgcgc gactgccgcg tatacgcctg aaccgttcga
     5821 atccgagggt tttatccatt gcacggacgg tgaagcgaac gtcgtcgccg tgggaaatcg
     5881 ctactacacg ggtgatccgc gtgggatcgt ctgcctggtc atcgattgcg aactcgttaa
     5941 tcaggaaatc aggtatgagg atccggagcg gatctacccg cacattttcg gtccgctaaa
     6001 cacgaacgct gttattgatg tgcggtcggt gcggcgcgag gcggacggct cattcacggg
     6061 aattggcgaa tctgtttcag aatgacgtct gtatcgtttt acgcaatcgt tttcgtcgat
     6121 gactgacacg agacagcctt ctcccgggcg ttcgatcttt cgtgtgctcg gcgccgcgat
     6181 gtaccggcac cggtttctgg tcgtttccct ctgggcgatc gcatttctga tctccatccc
     6241 gatgctcctg cgtgtcgagg agccgctgaa ggtcggcgga ttctcgtccg accgtacgga
     6301 agcggcccgt gccaggttga ccgtcgagcg cgagctttcg ggcagcgcgt cccagctggt
     6361 cgtgatcttc aagtcgacag atcagccgat tgccactccg gacgagcagc agcggatcgc
     6421 cgcggcgatc gagccgttcc ggggtcacgc gcacgtcatc gacgtcgttt tgccgtccga
     6481 gaacggtgcc cagatcagca aagacggccg gacagcctac gcactggtca gcctcgattt
     6541 accggccgag gaggcgcagc gcctgatccc ggatttcgag cgcttgatcg tcccgcagcc
     6601 gggcttgcag gttctgttgg ccggtggccc agccttctac gccgacatcg agacagtaag
     6661 ccagcgcgac ctgcagcgcg cggagctgat cgcgtttcca tttgcgcttg tcgcgctgct
     6721 ctttgtcttt gggacggcga tcgccgcgct gctgccgctg atcgtcggcg gtctcggcgt
     6781 cgcggcggtg ctgatgacca ttttcgggct ggcgcacgta acagatctct cgatcttcgt
     6841 gctcaatctc gcgacgatgc tggggcttgg cctggcgatc gactactcgc tcttcattac
     6901 gagccggttc cgcgaggaac ttccgcggtc cgagtcggtc gaacgcgcgg tcgagcggac
     6961 gatggtcacg gccgggcggg cgatcttctt ctccggactg acggtgctga tcgggctctc
     7021 gggcctgatc atgttcgatt tcatgttcct gcgctcggtc ggcattgcgg gtgtggttgt
     7081 cgtcttcttt tccgtgctgg ccgcgctaac gttgttaccg gcgctcctct cgattgccgg
     7141 gtcgaatgtc gagcgctggg cggtcatccg ccgatcgccg gacgaccatc atcggcacgg
     7201 cttttgggcg aggctctcgc gcgaggtgat gaagcgaccg gtgctgatcc tgatcccgac
     7261 cgcaatattg ctgcttgcgt tcggcgcgcc cttccgccac gtcaacattt cctctccgga
     7321 tgcgacgatc ctgcccaaga gcaccgactc ccgcaagggc ttcg
//
LOCUS       DATAOZ010000230         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1941513, whole genome shotgun sequence.
ACCESSION   DATAOZ010000230 DATAOZ010000000
VERSION     DATAOZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941513"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..1588)
                     /locus_tag="VHR64_06055"
     CDS             complement(350..1588)
                     /locus_tag="VHR64_06055"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302428.1"
                     /translation="MIVRQRRPVYSLIIANAISLVGSSLTAVAIPWFVLETTGSAGKA
                     GLSGAFAFLPAFFAGIFGGALVDRFGARHSAMVADLVSGSAILMVPVLYHTIGLQFWQ
                     LLILVLLGSLLDIPGVTARRALLPELAQLGGVRLERVNAMLEGNWQISILLGPPIAGV
                     LIGFLGAANVLWIDAASSLISFTAILLLVPANVHEHIRAVSQGYIRDLRTGLHFLWRD
                     QVLRWISLMLAISNAFGAPFFSLIMAVYIKERYDDPRYLGVLLSATGIGLLVGTTLYG
                     AVGYRWSRAHIMRASLLVLTISFWPFGFELPFPVILACVAIGGLADGPLNPLLVTVRL
                     ERIPVELRGRVFAATSAMAQMFPAFTIPLAGWMIEKIDLTPTVLILASCALVANLLLA
                     RLPVWDRLDESANREVAVPA"
     gene            complement(1585..2604)
                     /locus_tag="VHR64_06060"
     CDS             complement(1585..2604)
                     /locus_tag="VHR64_06060"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX3302429.1"
                     /translation="MSLDLDRLPRFPLANLPTPLQEAHRLRDALGGPDKCPRILIKRD
                     DLTGLAFGGNKVRKLEFLVGDALAKRATTLITAGAAQSNHARATAAAAVVAGLRSVLV
                     LTSTDAEPAVQGNLLLDELLGTEIRFITPDVHRDEHMATVAAELSEKGETPYVIPVGG
                     SNSIGAAGYLTMAVELKEQLAKIGAEPSRVYFGNGSGGTQAGVTLGVKALGIPARAIG
                     VQDSYLSPEDKQRIVRITNEAAELVGSDTRVTADDITDLDQYYGEAYGVPTREGDAAI
                     LLLARTEAIFLDSTYTGKAMSGLIDNIRTGEIRPDESVVFVHTGGNSALFAHAERLSG
                     LARAR"
     gene            complement(2611..3099)
                     /locus_tag="VHR64_06065"
     CDS             complement(2611..3099)
                     /locus_tag="VHR64_06065"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3302430.1"
                     /translation="MRAEGAFLAEQLVRQIHLVRRELRDLPDGDLNRVLDIQPTNTLF
                     QLGTHIAGSARYWAITNTGGANFHRDRDSEFNAIGTNAELLENLDALINQITDHVTCL
                     TAEQLEQPCSLPTASFSFWESVDPLPQRHALLHALEHTGLHLGHIQITRQILGYDPVK
                     EE"
     gene            3155..3874
                     /locus_tag="VHR64_06070"
     CDS             3155..3874
                     /locus_tag="VHR64_06070"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3302431.1"
                     /translation="MISDDDARIRIAQTANALGDDTRLRLLMLLLAAGSGGMTVTDLS
                     GRLSLAQPRVSTHLAILKDAGLVEVRTSGRTRVYRAVGERAMPLLNAVKRAAGVEDGP
                     PASPAALKEINRNSPNRSARSCYDHLAGVAGVELLDQFVFDGWLVRNGGPDERPIYEP
                     TEVGERGFSRLGIDLNQIRGGRRVLACGCLDWTERRPHLAGGLGAAVLASLESGGYVQ
                     RDPATRSVDVLRPIDDWLARA"
     gene            complement(3877..>4350)
                     /locus_tag="VHR64_06075"
     CDS             complement(3877..>4350)
                     /locus_tag="VHR64_06075"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX3302432.1"
                     /translation="EIVARRPDSIVIGADTTVEIGGSILNKPADLADAERMLRQLRGR
                     THRVHTGVAVLAPAQTTHAEVMTSRVTMRPFPDTELHAYLETGESLDKAGAYGIQGAA
                     GDIVDSVDGCYTNVVGLPLCAVARCLIAAGVEITVPASRCGFRDDRICPWWPIPE"
BASE COUNT          795 a         1340 c         1471 g          744 t
ORIGIN      
        1 gcttgccctt gttcttgtgg cccttgccgc ggttctccga tttgccgttg tggccgtggt
       61 tgtccttggc gatcccgggg tacgagaagg agggagtcga ggacattgcg agcacgccga
      121 gcgccagggc aagcgtgatg acgagtctga tcacgagtac ggggcgaggc aatttcatgc
      181 ggttctcctg acgcagcgtg gtccgcggga atcgccgagg cgcccgggtc gggaccggta
      241 aacgacctcg accttactat gtgccgcaat tgatcgctgt agcgcgcgac tcagaattgt
      301 gagccgctgc agccgaacga tcctgatcca cggacatgtt tgcgatgaat cacgcgggaa
      361 cagcgacttc gcggttcgcg gattcgtcga gccggtccca gaccgggagc cgggccaaga
      421 ggagattcgc gaccagggcg caacttgcga ggatgagaac agtcggggtg aggtcgattt
      481 tctcgatcat ccagccggcg agcggaatcg tgaacgcggg gaacatctgg gccattgctg
      541 aggtcgccgc aaagacccgg ccccgcagct cgaccggaat ccgctcgagc cgcaccgtga
      601 ccaggagcgg attcaacggg ccatctgcga gtccaccgat cgcgacgcag gccaggatga
      661 ccggaaaggg gagctcaaac ccaaaaggcc aaaagctgat cgttagcacg agcagcgatg
      721 cacgcatgat gtgcgccctg gaccagcgat acccgaccgc accatagagc gtagtgccga
      781 cgagcaggcc gattccggtg gccgagagga gcacgccgag atagcgggga tcgtcgtaac
      841 gctccttgat gtagaccgcc atgatcagtg agaagaaggg cgcgccaaac gcgttcgaga
      901 tcgccagcat cagggagatc cagcgcagga cctggtcccg ccagaggaaa tgcagccccg
      961 tccgcaagtc gcggatatat ccctgtgaca ccgcgcggat gtgctcgtgg acgttggccg
     1021 ggaccagcag gaggatggcg gtgaaggaga tcagcgacga ggcggcgtcg atccagagga
     1081 cgttggccgc gccgaggaag ccgatcagca ccccggctat cggcgggccg agcaggatcg
     1141 agatttgcca gttgccctcc aacatcgcgt tgacgcgttc aaggcgcacg ccgccgagct
     1201 gggccagctc cggcaggagc gcgcgccgcg cggtgacgcc cgggatatcg aggagcgatc
     1261 ccaacagcac gaggatcagg agctgccaga attgcagacc gatcgtgtgg tagagcacag
     1321 gcaccatcag gatcgcgctg ccgctaacca gatcagccac catcgcactg tggcgcgcgc
     1381 cgaatcgatc taccagcgcg ccgccgaata tccccgcgaa gaaggcgggc aggaaggcga
     1441 acgcgccgga caagcccgcc ttgccggcgc tcccggttgt ctccagcacg aaccacggga
     1501 tcgcgaccgc ggtcagcgag ctgccgacga gcgaaatcgc gttggcgatg atcagcgaat
     1561 agaccggcct gcgttgtcgg acgatcaccg cgctctggcg agccccgaga gtcgttcggc
     1621 gtgggcgaag agcgccgagt tgccgcccgt atggacgaat accaccgact catcggggcg
     1681 gatctcgccg gttcggatgt tgtcgatcag gcccgacatc gcctttccgg tataggtcga
     1741 gtcaaggaag atcgcttccg tgcgcgccag aagcaggatc gccgcgtcgc cttcgcgtgt
     1801 tggcaccccg tacgcctcgc cgtagtactg gtccaggtcg gtgatgtcgt ccgcggtcac
     1861 gcgcgtgtcg ctaccgacaa gctcagccgc ttcgttcgtg atgcgaacga ttcgctgctt
     1921 gtcttcgggg gagaggtagc tgtcctgcac accaattgcg cgggccggaa taccgagcgc
     1981 cttgacgccg agtgtgacgc ccgcctgggt tccgcccgat ccattaccga agtagacgcg
     2041 gctcggctcg gctccgatct ttgcaagctg ttccttgagc tcgacggcca tcgtcaggta
     2101 cccggccgcg ccgatgctgt tcgaaccgcc gactggaatg acatacggcg tctcgccctt
     2161 ctcgcttaac tcagcggcga cggtcgccat atgctcatca cggtggacgt cgggcgtaat
     2221 gaaccggatc tcagtgccga gaagctcatc gagcaggagg ttcccctgca ccgccggctc
     2281 cgcgtcggtc gacgtcagca caaggacgga tcgcaggccg gccaccaccg cagccgcggc
     2341 agtggcgcgc gcatggttgg attgagcggc tccggccgtg atcagggtgg ttgctcgctt
     2401 ggcgagtgcg tcgccgacca ggaattcgag cttgcgaacc ttgttgccgc caaaagccag
     2461 gccggtcagg tcatcccgct tgatcagaat gcgcgggcac ttgtcgggtc cgccgagcgc
     2521 gtcgcggagt cggtgtgcct cctggagcgg cgttggcagg ttggcgagtg ggaaacgtgg
     2581 caatcggtcg agatcgagcg acacgtgtgc ttactcctct ttcactggat cgtacccaag
     2641 gatctgtcgc gttatctgaa tatgtcccaa atgcaggccg gtgtgctcca gggcatggag
     2701 caaggcgtga cgctgcggaa gtgggtcgac gctctcccag aaggagaagg atgcggttgg
     2761 cagcgagcag ggctgctcca gttgctcggc ggtcaggcac gtgacatggt cggtgatctg
     2821 attgatcaag gcatcgaggt tttccagcag ttccgcgttg gttccgatcg cgttgaattc
     2881 cgaatcgcga tcgcgatgaa agttggcgcc gccggtgttg gtgatcgccc aatagcgtgc
     2941 cgagccggcg atgtgcgttc cgagctggaa gagcgtgttg gtgggttgaa tgtcaagtac
     3001 ccggttgagg tccccgtccg ggagatcgcg caactcgcgc cgtacgagat gaatctgccg
     3061 gaccagctgc tcggccagga atgcgccttc tgctcgcatt ggtacaggac ctttcgctct
     3121 tgacgaatat gtgcccgcga tctagtctga cggcatgatc agcgatgatg acgcccgaat
     3181 ccggatcgcg cagacggcaa acgccctggg ggacgatacc cgattgcggt tgctgatgtt
     3241 gctgctcgcc gcggggtcgg gtggaatgac cgtgacagac ctctccggcc ggttgtccct
     3301 ggcgcaacca cgtgtctcga cgcacctcgc gatcctgaag gacgcgggac tggtcgaggt
     3361 gcgaacttcc gggcggacgc gggtctaccg ggccgtgggc gagcgggcga tgccgctgct
     3421 gaacgctgtg aagcgcgcgg ccggggtcga ggacggccca ccggcaagcc cggcggccct
     3481 caaggagatc aaccgcaaca gcccgaaccg cagtgcccgg agctgttacg accacctggc
     3541 cggcgtagcc ggggtcgagc tgctcgatca gttcgtgttc gacgggtggc tggtgcgtaa
     3601 tggcggaccg gacgagcgcc cgatctatga gccgaccgaa gtcggcgaac gcggattttc
     3661 cagattggga atcgacctga accagatccg gggcggtcgc cgggtactgg cctgcggatg
     3721 ccttgactgg accgagcggc ggccgcacct ggccggtggc ctgggtgcgg cggtgttggc
     3781 gtccctcgaa tcaggtggat acgtgcaacg cgaccccgcg acacgcagcg tcgacgtact
     3841 gcgcccaatc gacgactggc tcgcccgagc ctgacctcac tccgggatcg gccaccacgg
     3901 gcagatccgg tcgtcgcgga acccgcagcg ggatgctggt acggtaattt ccacacccgc
     3961 cgcgatcaga caacgcgcga cggcacagag cggcaacccc acgacattcg tatagcaccc
     4021 atccaccgag tcgacgatgt ccccggctgc gccctggatc ccgtaggcgc cggccttgtc
     4081 gagcgactcc ccggtctcca gataggcgtg caactcggtg tcgggaaacg gccgcatcgt
     4141 cacccgggac gtcatgacct cggcatgggt cgtttgagcc ggcgcgagga ctgcgacgcc
     4201 ggtgtgtacg cgatgcgtgc gccctcgcaa ctggcgcagc atccgctcgg catccgcgag
     4261 atcggccggc ttgttcagga tggagccgcc gatttcgacc gtcgtgtccg ccccaatcac
     4321 gatggagtct ggtcgccggg ccacgatctc
//
LOCUS       DATAOZ010000231        11882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4571473, whole genome shotgun sequence.
ACCESSION   DATAOZ010000231 DATAOZ010000000
VERSION     DATAOZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11882
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4571473"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            658..1617
                     /locus_tag="VHR64_06080"
     CDS             658..1617
                     /locus_tag="VHR64_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX3302433.1"
                     /translation="MQCPAVSCDFVIWLDGQPVSRALVGGKGASLSNLIALGAPVPPA
                     FSLSTDAYAAMAEAAGLPKRASDVSDDDLPAIRAVIEASPLPADIRAALTEAFAELSI
                     LAGGEISVAVRSSATAEDSAVFSFAGLHDTILDVRDYDALEEAVKRCWASLWTERAVA
                     YRRQGGMDADEGGIAVVIQQLVRSDVSFVAFAADPISGQTDYVLINASWGLGEALVSG
                     MVTPDQVIVGSDGAIHEYLIGDKAEMIVPRADAPGTQTVPVPRLLRSLPALNREQIDA
                     ISAMTRAMSDELGYPADLEGGLVGSQIMLFQARPITTLGMVAA"
     gene            1672..3339
                     /locus_tag="VHR64_06085"
     CDS             1672..3339
                     /locus_tag="VHR64_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX3302434.1"
                     /translation="MVAAVSTDSIPFAPTLTDTVEAPLTYFADPMHFPRPLSPLFVSV
                     HGPSFERGFTKAVRELNVPIRETRVKVRNHYYFNAMIPAVPANEVEAREMGQRAEATL
                     KVEAGRMMERWTTEHLPLIKWRLHRIETMNVAEAEPADVVDMLDELQTIWAENWATHF
                     RIVIPMMLAKQLFDELYVDLFGGTEQDAHALLIGAESESIKAGMGLSDLAGSARAAGL
                     TDLLLSMPAEKVPAALARHSMGGYFSAAIRDYLREYGLRQDLFDFTTPTWREDPSIVI
                     ALVQAYLRNGHDARAEHAASTVLAEKAQIEARQQLAVYPEAVRGQFAGMLQAARTAYF
                     LQEEHNFYIDQQMIALTRLLFLKLGERLVTEGALVEAADIFMLSVDEVKAAVFGDQES
                     ARRIVPQRMLELMDAAQEPPPPFIGPAPAGPPPADNLIERAMGRFFGGPPQQAEQPNQ
                     LKGNPGSRGTARGVARVVQTLEEAKALQPGEILVTITTMPAWTPLFGTAAAVVTETGG
                     ALSHCAIVAREYGIPAVVGVYGATRTIQTGQTISVDGTSGLITLEAE"
     gene            3421..4998
                     /locus_tag="VHR64_06090"
     CDS             3421..4998
                     /locus_tag="VHR64_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3302435.1"
                     /translation="MRYETRYARSGDTHIAYQVIGDGPLDLVFVMGWISHLDYFWEGP
                     SADFLNRLASFSRLILFDKRGTGLSDRVAELPSIEQRMDDVRAVMDAADSQRAALLGI
                     SEGAAMCALFAATYPERTTALVMYGAYAKRLRDPDYPWAPTTEQRQRFFDAIEQGWGG
                     VVDLETLAPSAAGDERFRQWWATYLQRSASPGAALALARMNTAIDIRHVLPAIRVPTL
                     ILHRTGDLDIDVGGARYMAERIPGATYVELPGNDHLVFAGDQEAVLGEIEQFLTGVRP
                     ATEPDSVLATILLTEIIGAVAIAARLGAGGWAEALGAHERMVRDEIARFRGQEVSSTA
                     SGIVAIFDGPARAVRCAASIVERARGAGIDVRAGLHTGECEVMDGEIRGIAARIAAEV
                     LMQAERGTVVVSSTIPDLLAGSGITFHLLDQSVATGTGRILRLYQVLTTRTPGEAIGQ
                     SSKPTERGSLTPRELEVARLIALGQSNREVAEALFISVATVERHVANIFTKLDLRSRT
                     QLGVWIAEHGLIAPDQA"
     gene            5130..5801
                     /locus_tag="VHR64_06095"
     CDS             5130..5801
                     /locus_tag="VHR64_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302436.1"
                     /translation="MHATVTTWCLRHPPRDTAEFDRRVRALMKGGIELGRRIGVVDVL
                     IVAFDPNMLLIVTLYETLDEAMEAGPHVLHYVREGFADQLELVSRVAGQAFETSQFAP
                     VDRDETQQWRLDAAQMYGNAATWHLDPSIRSPEAFEAFVSDVMAKSYERLKNRGLLDA
                     FGVRISNDELLVLRLCSDPAIFDAIYSEESIAAGQALLNGKISLVEQSTGRAFDAVSI
                     FNEPA"
     gene            5922..7127
                     /locus_tag="VHR64_06100"
     CDS             5922..7127
                     /locus_tag="VHR64_06100"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX3302437.1"
                     /translation="MSLRRVFVIPVLVTLLMIGAPLWAVAQQATPMPMPGTVIASGLT
                     NPRDFTWGADGTLYVALAGTGGSTPATEDSPANKAIGPFMGGSTGALVRIENGCPTAV
                     VTGLPSVADGIGSVLGAEDVAILGDHLYVSSDGGGAAHGNPDAPSGIYRALADGTFQL
                     VADLSAFLRANPAKIGTSFPDYDPDADGYRMVADESANLLWVLEPNSGGLLSVTADGT
                     ITRVADLSDGHPVPASIALAPGGGVYIGNLTAAPFPDGTAKVIKVMPDGTVTDVLTGL
                     TTVTGLAVASDGTLYVLEMSTGNTDQPPFLVPGSGKVLRMTGADSTEEVASGLMLPIS
                     LNIGPDNAFYVSMPAVGANDGSGSIVRIESGMAGTPMAGMTEAPASCTPLPETMATPG
                     ATPMASPSS"
     gene            7447..7881
                     /locus_tag="VHR64_06105"
     CDS             7447..7881
                     /locus_tag="VHR64_06105"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3302438.1"
                     /translation="MARTSTYLHFSGTAEEAFNFYKAAFGTEFVGPIMRMGDLPAQPG
                     QPELSAEEKNQCMSIKLPITGGHLLVGNDAPAWMGTVVHGNAVDIGVELDTRAEADRI
                     FTALSEGATVEYPLQEMFEGDYFGSLVDKFGTQWLVSCPAKS"
     gene            complement(7949..9211)
                     /locus_tag="VHR64_06110"
     CDS             complement(7949..9211)
                     /locus_tag="VHR64_06110"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX3302439.1"
                     /translation="MLVVMESGTSDGAVTAVRDRAREAGLDTTLYAGNDPAIIQITGD
                     NADELEPDLSALPGVAQVMCATSSAPPVTSNLRIAGIRPLVPPAILMEQLPLPAEGAV
                     LVHRTRQEMRQILAGRDDRLIVVVGPCSVHDTDAALEYARRLKPLADELKDDLRIVMR
                     VYFEKPRTTVGWKGLINDPNLDGSFAVNEGLYLARQLLLDVIAEGLPAGTEFLDPITP
                     QFIADAVSWGAIGARTTESQVHRNLTSGLSMPVGFKNGTGGGIQMAIDAMQAASYPHQ
                     FMSVTEQGLAAIVVTRGNRDTHVILRGGKSGPNYDSEHVQKTLGMIRSSGMPARVMID
                     ASHGNSEKDYRRESVVSQAIAEQVAAGERGIIGVMLESFLVDGRQDLHDPHHLVEGQS
                     ITDACMGWDMTVPVLKDLAKSVRKRRTG"
     gene            9355..11013
                     /gene="nhaA"
                     /locus_tag="VHR64_06115"
     CDS             9355..11013
                     /gene="nhaA"
                     /locus_tag="VHR64_06115"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX3302440.1"
                     /translation="MAEAEMNEVNGPGVTISVSLRIEPGRESEFDEYLAGITHAAEEF
                     PGFMSVQAYRPTRGRGRYRVLLRFDSERSLQRWRESDERTIWNERADEISEGTPRILN
                     ITGTAQEQPLALALGPLQEFVRNSVSGIGLLLLGAALALIMANSRWADTYESFWNTKV
                     TIGSADFGITVTLRHWVNDALMALFFFILGLEIKREVLVGELREIRQAALPIAAAIGG
                     GVVPAAIYALINIRGDALHGWGIPIGTDTAFALGILSILGGRVPTLLVVFLTATSIVD
                     DIIAVGVIAIFYTDNLNVTAMGVAVLLLGALVLANRAGVHRWYIYGILGIGVWLAVFE
                     SGVHGTIAGVLVAMTVPARSWINSGDFVRVGRRLIDEFEASCAQGENILSNERQQLAA
                     QRLDRLVEGVETPMSLMEHGLGPWVSYLVLPLFAFANAGIPLRSGLGDALGSTVTWGV
                     ILGLLIGKPIGITLFSWLSVHSGIAHSPQGITFHDIFGVAGLGGIGFTMSLFITELAF
                     GTDPAANNARIGVIIGSIISGTIGYLILSRTLKHRSNGEAVNST"
     gene            11087..11362
                     /locus_tag="VHR64_06120"
     CDS             11087..11362
                     /locus_tag="VHR64_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302441.1"
                     /translation="MFQRLVGIIFGAIVAFLLLLLFHNGRLISDERSGFLIAVAAGAV
                     ANMLWPLIWAGLIARRNRVNRQATVQAEVQRQVNTQRSTEPPTPPTE"
     gene            complement(11398..>11882)
                     /locus_tag="VHR64_06125"
     CDS             complement(11398..>11882)
                     /locus_tag="VHR64_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302442.1"
                     /translation="RGSLARRGLQVIMHNTLASSDYGLLDENTFAPRPNYWATLLWRD
                     LMGTTVLDPGPSPAANLYLLAHSLRGTEGGVAVVAVNADRATSHTLEVAVPVERYTLT
                     ATGLQDSVVQLNGTDLQLSPDDSLPPLAGATSRPGPLVLAPLSITFLAMPTASNNACR
                     "
BASE COUNT         2015 a         3877 c         3824 g         2166 t
ORIGIN      
        1 aaaggaccgg gctgaccaac ggaaagccca ctgaaggggc tgaagtggtg ttccgatggc
       61 aacgtgggac tgtcgacgcc gccccggact gaaggccggg gctgaagagc aataaggcgg
      121 ctgaagccga ctcccttttt agccccttta gtgggctttc cgttggtcag cccgggcatt
      181 tatccccggg cgatgccggg aaaccagttc gaggatacga atccggtcca cggctcctcc
      241 atcatgccgc ttcgcgtcat cgcgggatgc tctctcccta tcagggtgag ccggagaggg
      301 tggtgaggca cccgcaccac atcaaatggc gccgatctcc gtacgtttct taggcaaatc
      361 tcacggaaca cgcaattgcg tctccgtcga aaggctgagg ttcgggtcag tcactcgccg
      421 catgtgcatc gatctcccaa tcggtacaac tgtatcgatg aagtaacccc gactgccgtc
      481 gaccagcgaa cgctcgcggc ctccattgac cgcccgtttt cgttgctcaa cgcctgttgc
      541 ggatcccgga cgacccggga ttcgtcgccc gccgaatcca atcgacggac gattgcggcc
      601 gattcggacg cgtatcgccg gatccggtaa tgaggccgga accgagggag aacgaccgtg
      661 cagtgtccag cagtttcgtg cgatttcgtg atctggctcg atggccaacc ggttagccgg
      721 gcgctcgtcg gcggcaaggg tgccagcctg agcaacctga tcgctctcgg cgcccccgtt
      781 ccgcccgcct tctcgctctc gaccgacgcc tacgccgcga tggccgaggc cgccggtctg
      841 cccaaacgag ccagcgatgt gtctgacgat gacctgccgg cgatccgggc cgtcatcgaa
      901 gcctccccgc ttcccgccga catccgcgct gcgctcactg aagcattcgc cgagttgtcc
      961 atcctggccg gtggcgagat ctcggtcgcg gttcgctctt ccgccaccgc cgaggattcg
     1021 gccgtcttct cctttgccgg attgcatgac acgatcctcg atgtccgcga ctacgacgcg
     1081 ctcgaggagg cggtcaagcg ctgctgggcg tcactctgga ccgaacgggc cgtagcgtac
     1141 cgccgccagg gtggcatgga cgccgatgag ggcgggatcg ccgtcgttat ccagcagctc
     1201 gttcgcagcg atgtctcgtt cgtcgccttc gcggccgatc cgatctcggg ccagaccgac
     1261 tacgtgctga tcaacgcctc ctggggactg ggcgaagcgc tcgtctccgg catggtcacg
     1321 ccggaccagg tgatcgtcgg ctccgatggc gcgatccacg agtacctgat cggcgacaag
     1381 gccgagatga tcgtgccccg cgccgatgca ccgggcacgc agaccgtccc ggtgccgcga
     1441 ctcctgcgct ccctcccggc tctgaaccgc gagcagatcg acgcgatctc ggcgatgact
     1501 cgcgccatgt ccgacgagct gggttacccg gccgatctcg aaggcggatt ggtcggctcg
     1561 cagatcatgc tcttccaggc tcggccaatc acaaccctcg gcatggttgc cgcgtaatac
     1621 cccactcgat tggcacatcg aacgtcacaa catcatcaag gagcccattc gatggttgca
     1681 gcagtttcca ccgattcgat cccgtttgcc ccgacgctga ccgatacggt cgaagcaccc
     1741 ctgacctact tcgcggatcc gatgcacttt ccccgaccgc tcagcccgct cttcgtgagc
     1801 gtacatgggc cgtcgttcga gcgcggcttc acgaaggcag tgcgggagtt gaatgtccca
     1861 atccgcgaga cccgcgtcaa ggtgaggaac cactactact tcaacgccat gattccggca
     1921 gtcccggcca atgaagttga ggcccgggag atgggccagc gcgccgaggc gaccctgaag
     1981 gtcgaagccg gccggatgat ggagcgctgg acgacggagc acctgccgct gatcaagtgg
     2041 cgcctgcacc ggatcgagac catgaatgtc gccgaagccg aaccggctga tgtcgtcgac
     2101 atgctcgatg agctccagac gatctgggcc gagaactggg cgacgcactt ccggatcgtg
     2161 atcccgatga tgctcgccaa gcagctcttc gacgagctct acgtcgacct cttcggcggc
     2221 accgagcagg acgcccacgc gctgctgatc ggggccgaat cggagagcat caaggccggt
     2281 atgggcctct cggatctggc cggctcagcc agggccgccg gcttgaccga tctcctgctc
     2341 tcgatgccgg ctgaaaaggt cccggcagca ttggccaggc actcgatggg cggctacttc
     2401 tcggccgcga ttcgtgacta cctgcgcgag tacggcctgc gccaggacct gttcgatttc
     2461 acaacgccga cctggcgcga ggatccctcg atcgtgatcg cgctggtcca ggcatacctg
     2521 cggaacgggc atgacgcccg cgccgagcat gccgcgtcga ccgtactggc cgagaaggcg
     2581 cagatcgagg cgcggcagca gctcgcggtc tacccggagg cggtccgcgg tcagttcgct
     2641 ggcatgctcc aggcggcgcg aacggcctac ttcctccagg aggagcacaa cttctatatc
     2701 gaccagcaga tgatcgccct gacccgcctg ctcttcctga agcttggcga gcggctcgtg
     2761 accgaaggcg cgctggttga agccgcggac atcttcatgc tctccgtcga cgaggtgaag
     2821 gcggcggttt tcggcgacca ggaatcggcc cgccggatcg tcccgcagcg gatgctggag
     2881 ctgatggacg ccgcacagga gccaccaccg ccgttcattg gtccggcccc ggcgggtccg
     2941 ccgccggccg acaatctgat tgagcgggcg atggggcgct tcttcggtgg cccgccgcag
     3001 caagccgagc agccgaacca gctcaagggc aatcccggct cacgcggcac ggcgcgaggg
     3061 gtagcccggg tcgtccagac cctcgaggag gccaaggccc tgcaacccgg cgagatcctg
     3121 gtcacgatca cgaccatgcc ggcctggacg ccactgttcg gcacggccgc ggcggtcgtg
     3181 accgagacgg gtggcgcgct cagccattgc gcgattgtcg cccgcgagta cggcatcccg
     3241 gcggtggtcg gcgtctacgg cgctacccgc acgatccaga ccggccagac catctcggtc
     3301 gacggcacca gcggtctgat caccctcgag gccgagtaac tcatcctccc tcccagccac
     3361 gcggcccctc caccagccag gaggggtcgt ttcctttgcc ccggcagtac gatacgaggc
     3421 atgcgatacg aaaccaggta cgccagaagc ggtgatacgc acatcgccta ccaggtaatc
     3481 ggggacggtc ccctcgatct cgtgtttgtc atgggctgga tctcgcacct cgactatttc
     3541 tgggaaggac caagcgccga tttcctgaat cgcctcgcct cgttctcgcg gttgatcctg
     3601 ttcgacaagc gggggaccgg cctttccgac cgggtcgctg agctgccctc gatcgagcag
     3661 cgcatggatg acgtacgcgc ggtgatggat gccgccgact cccagcgcgc ggccctgctc
     3721 ggcatctccg aaggcgccgc catgtgtgcg ctctttgccg cgacctaccc cgaacgcacg
     3781 acggcactgg tcatgtacgg tgcgtacgcc aagcgactcc gggatcccga ttacccgtgg
     3841 gcgccgacga ccgagcaacg acagaggttc ttcgacgcaa tcgagcaggg ttggggcggc
     3901 gtggtcgatc tggagacgct cgccccgagc gcggccggcg acgagcgttt ccggcagtgg
     3961 tgggcgacct atctgcaacg gagcgcgagc cccggcgcgg cgcttgccct ggcgcggatg
     4021 aacacggcga tcgatatccg gcacgtgctt ccggccattc gcgtcccgac tttgatcctg
     4081 catcgtaccg gcgatctcga cattgacgtg ggtggcgcgc ggtacatggc cgagcgtatc
     4141 cccggagcca cctatgtcga gctgccgggc aacgaccatc tcgttttcgc cggcgaccag
     4201 gaggccgtgc tgggcgagat cgaacagttc ctgacgggag tacgccccgc gactgagccg
     4261 gactctgtgc tggcgacgat cctgctgacc gagatcatcg gcgctgtggc catcgcagca
     4321 cgacttggcg cgggaggttg ggccgaggcg ctcggtgcac acgagcgcat ggtgcgggac
     4381 gagattgcgc ggttccgggg gcaggaggtc agctcaacgg cgtcaggaat tgtcgcgata
     4441 ttcgacggac cggcccgtgc cgtccgctgc gcggcctcga tcgtcgaacg ggcgcgaggc
     4501 gccggcattg atgtccgtgc cgggttacat acgggcgagt gcgaggtcat ggacggcgag
     4561 attcgcggga tcgccgcccg gattgcggcc gaggttctga tgcaggccga gcgcggcacg
     4621 gtggtggtct ccagcacgat tcccgatctg ctggccggat ccggtattac cttccatctg
     4681 ctcgaccaga gcgtggcgac cggcaccggc cggatcctgc gcctgtatca ggtcctgacg
     4741 acgcgcactc caggtgaggc gatcggccag agtagcaagc cgactgagcg tgggtcgctg
     4801 acccctcgag agctggaagt ggccaggttg atcgcgctcg ggcagtccaa ccgcgaggtc
     4861 gccgaagcgc tcttcatctc ggtcgccacg gtcgagcgcc atgtggccaa catctttacc
     4921 aagctcgatc tccggtcccg cacgcagctc ggtgtctgga tcgctgagca cggattgatc
     4981 gcgcccgatc aggcctgacc cgccgcgcac gtgcgttgcg gcacgtaatc cgccggaatg
     5041 ggacggtggg atgggcggcc tatggtcacc gccccatgga tcactacaat agaggtcgca
     5101 accggcagaa aactgatggg gcgatggcca tgcacgcgac cgtcacaacc tggtgtttgc
     5161 gtcaccctcc gcgcgatacc gccgagttcg accgccgtgt ccgggccctg atgaagggcg
     5221 ggatcgagct tggacgccgg atcggtgtgg tcgatgtgtt gatcgtcgca ttcgacccga
     5281 acatgcttct gatcgtcacc ctctacgaga cccttgacga agcgatggag gcgggtccac
     5341 acgtgctcca ttacgtcagg gagggcttcg ctgaccagct cgagctggtc agccgggtcg
     5401 caggccaagc cttcgaaacc tcccagttcg cgcccgtcga tcgggacgaa acccagcaat
     5461 ggcgcctcga tgcggcgcag atgtacggca acgcggcgac ctggcatctt gatccatcga
     5521 tccgctcgcc cgaggcattc gaggccttcg tgagcgacgt catggcgaag tcatacgagc
     5581 gtctgaagaa tcgtggcctg ctcgatgcat ttggcgtccg catcagcaac gacgagctgc
     5641 tcgtgcttcg gctgtgcagc gatcccgcga tcttcgacgc catctacagc gaggaatcga
     5701 ttgcagccgg gcaagctctg ctgaacggga agatttccct ggtcgagcag agcaccgggc
     5761 gtgccttcga cgccgtctcc atcttcaacg agcctgcctg aaccctgccg gaaacgcccc
     5821 aatttccgcg gtaaacgtcg gttgcgatga cttcccgtca agagtaggat gggcaaaccg
     5881 ccatattctc ggtgacgggc gaacaacgca gggaggaagt tatgtcgctt cgacgcgttt
     5941 tcgttattcc cgtactcgtc acgctcttga tgatcggcgc cccgctctgg gccgtggccc
     6001 agcaggcaac gccgatgccc atgccaggca cagtcatcgc cagcgggttg accaacccgc
     6061 gcgatttcac ctggggtgcg gacgggacgc tttacgtcgc tctggcaggt acaggcggaa
     6121 gcacgcccgc cacagaggat tcaccggcga acaaggcgat cggtccattc atgggcggat
     6181 cgaccggcgc gctggtccgg atcgaaaatg gctgcccgac cgcggtcgtg accggcctgc
     6241 cctccgtggc cgatggtatc ggcagcgtgc ttggcgcgga agatgtcgcc atcctcggcg
     6301 atcacctcta tgtctcgtcg gacggcggtg gagcggcgca cggtaatccc gatgccccga
     6361 gcggtatcta tcgcgctctc gccgacggca ccttccagct agtggccgac ctctccgcct
     6421 tcttgcgcgc caacccggcc aagatcggca cctcgttccc ggactacgat ccggatgctg
     6481 atggctaccg gatggtcgcc gacgaatccg ccaatctgct ctgggtcctc gagccgaact
     6541 ccggcggcct tctgtccgtg acagcggacg gcacgatcac ccgcgtggct gatctctccg
     6601 atggtcaccc ggttcccgcg tcaatcgcgc tggccccggg cggcggcgtc tacatcggca
     6661 acctgaccgc ggctcccttc ccggacggca cggccaaggt aatcaaggtc atgccggacg
     6721 gcaccgtcac cgatgtcttg accggtctga ccaccgtcac cggacttgcg gtcgcctcgg
     6781 acggcacgct ctacgtgctc gagatgtcga ccggcaatac cgatcagccg ccgttcctgg
     6841 tgccgggttc gggaaaggtc ctgcgcatga ccggcgccga cagcaccgaa gaggtcgcga
     6901 gcggtctgat gttgccgatc tcgctcaata tcggaccgga caacgccttc tacgtctcga
     6961 tgccggcagt cggtgcgaac gatggttcgg gctcgatcgt ccggatcgag agcggcatgg
     7021 ccggcacccc gatggcgggc atgactgagg ccccggcctc ctgcactccg ctcccggaga
     7081 cgatggcgac tccaggcgcc acaccaatgg cctccccaag ctcgtaatcg acggtcgtca
     7141 atcggacacg ccggggccgc gcaatttgcg cggccccggc ctattgcagc gaattgcatt
     7201 gagcatgcat ccgcaccact tccaatcgct ctgttgagtg gtgacgcccg gactgacgtc
     7261 accgtcactg cggcagggga caggcagtgc ggaaatgctc gcgatgcgat tgtcgatttc
     7321 gggtagtgcc ggtcgactat tgaatggaag cggcagtgag tgaatggtgg agtcgtttcc
     7381 gcgggggtgc cgaacgtttc ggcatcaatc cgtgaaccaa cccatatcgc attccaggag
     7441 gcacacgtgg ctcgcaccag cacctatctc cacttcagcg gcaccgccga ggaggcgttc
     7501 aatttctaca aggcggcatt cggcaccgag ttcgtcggcc cgatcatgcg gatgggcgac
     7561 ttgccggccc agcccggtca gcccgagctg agcgccgagg agaagaacca gtgcatgagc
     7621 atcaagctcc cgatcaccgg cgggcacctg ttggtgggta acgatgcgcc ggcgtggatg
     7681 ggcaccgtcg tccacggcaa cgccgtcgac atcggcgtcg agctggacac gcgcgccgag
     7741 gccgaccgca tcttcaccgc gctctccgaa ggcgccaccg tcgagtatcc gctccaggag
     7801 atgttcgagg gcgattactt cggctcgctg gtcgacaagt tcggcacgca gtggctggtc
     7861 agctgtcccg cgaaatcgta gccacgaact aacgtcacat gctgaatacg aaccggcgag
     7921 ttcggttcac agtcggtgcg tggcgcggct acccggtacg gcgttttcgg acggacttgg
     7981 cgaggtcttt gaggacgggg acggtcatgt cccagcccat gcaggcgtcg gtgatgcttt
     8041 ggccctcgac caggtggtgc gggtcgtgca gatcctggcg gccgtcgacc aggaagctct
     8101 cgagcatgac gccgataatg cctcgttcgc cggcggccac ttgctccgcg atcgcttgcg
     8161 agactaccga ctcgcggcgg tagtccttct cgctgttgcc gtggctggcg tcgatcatca
     8221 cccgcgccgg catcccgctc gaccggatca tcccgagcgt cttctggaca tgctcggagt
     8281 cgtaattcgg accgctcttg ccgccgcgaa ggattacgtg cgtgtcccgg ttgcctcgcg
     8341 tgacgacgat cgccgccaac ccctgctcgg tcacgctcat gaactggtgc gggtacgatg
     8401 cggcctgcat cgcgtcgatc gccatctgga tcccgccacc ggtgccgttc ttgaagccga
     8461 ccggcatcga gagacccgag gttagattgc gatggacctg gctctcagtc gtccgcgcgc
     8521 cgatcgcgcc ccaactgacg gcatcggcga tgaactgcgg agtgatcgga tcgaggaact
     8581 cggtcccggc cggcaatccc tcggcgatca cgtcgagcag cagctgacgg gccaggtaca
     8641 gcccctcgtt gacggcaaag ctgccatcga ggttcggatc gttgatcaga cccttccagc
     8701 cgacggtcgt gcgcggcttc tcgaagtaga cgcgcatcac gatccggaga tcgtccttga
     8761 gctcgtcggc cagcggcttc agccggcgcg cgtactccag cgccgcgtcg gtatcgtgga
     8821 ctgagcacgg gccgaccacg acgatcagcc gatcatcccg gccggcgagg atctgtcgca
     8881 tctcctgccg cgtccggtgg accaggacgg cgccctcggc cgggagcgga agctgctcca
     8941 tcaggatcgc cggcggcacc agcggccgga tcccggcgat gcgcaagttg ctggtcaccg
     9001 ggggagccga agaggtcgcg cacatgacct gggccacgcc cggcaatgcc gaaagatccg
     9061 gctccagctc gtcggcattg tctccggtga tctggatgat cgcggggtcg ttcccggcgt
     9121 agagcgtcgt gtcgaggccg gcctcccgcg cgcggtcgcg gacggctgtt accgccccat
     9181 ctgacgtacc cgattccatc accaccagca ttgtgccgtt cctctcaagc ccaacaaggt
     9241 caatgcggca tattttcgtt ccacggatgc cgttccgtca gcccgtattt cgcaatcacc
     9301 cctcaataat cgggtaaagt gaagccttca ggctcacatc atggggagaa tgcaatggca
     9361 gaggcggaga tgaacgaggt caacggccca ggcgtgacga tcagtgtctc gctgcggatc
     9421 gagccgggac gcgagtcgga gttcgatgaa tacctcgccg gcatcaccca cgccgccgag
     9481 gaattccccg gcttcatgtc ggtccaggcc tatcgcccga ctcgcggccg tggccggtat
     9541 cgagtcctcc tccgcttcga tagtgagcgc agcctgcagc gctggcgcga gtctgacgag
     9601 cgcacgatct ggaacgagcg ggccgacgag atttccgaag gcacgccgcg catcttgaac
     9661 atcaccggca cggcgcagga gcagccgctg gcgctggcgc ttggcccgct ccaggaattt
     9721 gtccgaaata gcgtttcggg catcgggctc ctgctgctgg gggcggcgct ggcgctgatc
     9781 atggccaact cgcggtgggc ggatacatac gagagcttct ggaacacgaa ggtcacaatc
     9841 ggctcggccg atttcgggat cacggtcacc ttgcgccact gggtgaatga cgccctgatg
     9901 gcgctcttct tcttcatcct cggacttgag atcaagcgcg aagtgctggt cggcgagctt
     9961 cgcgagattc gccaggcggc gctcccgatc gcggcggcga tcggtggcgg tgtggtgccg
    10021 gccgcgatct acgcgctgat caacatccgg ggcgatgcgt tgcatggctg gggcatcccg
    10081 atcgggaccg acacggcctt cgcactcggt atcctgagca tcctcggcgg gagggtgccg
    10141 accttgctgg tcgtctttct gaccgccacc tcgatcgtcg acgacatcat tgccgtcggt
    10201 gtgattgcga tcttctacac cgacaacctc aacgtgacgg cgatgggcgt cgctgtcctg
    10261 ctgctcggtg cactggtgct ggccaaccgg gctggagtcc accgatggta tatctacggg
    10321 attctcggaa ttggggtctg gctggcggtt ttcgagtccg gcgtgcacgg gacgattgcg
    10381 ggcgtgctgg tcgcgatgac ggtaccggcg agatcctgga tcaattcggg cgatttcgtc
    10441 cgcgtcggcc ggcggctgat cgatgagttc gaggcctcct gcgcacaggg cgagaatatc
    10501 ctcagcaacg agcggcagca actcgccgcg cagcgactgg atcggttggt cgagggcgtc
    10561 gagacgccga tgagtctaat ggagcatggg ctcggcccat gggtctcgta cctggtgctg
    10621 ccgctctttg ccttcgccaa cgccggcatt ccgctgcgga gcggcttggg cgatgcgctc
    10681 ggcagcacgg ttacctgggg cgtcatcctc ggcctgttga tcggcaagcc gatcggtatc
    10741 acgctgttct cgtggctctc ggtgcacagc gggatcgcgc attcgccgca aggcatcacg
    10801 ttccacgata tcttcggcgt ggccggactg ggtggtatcg gtttcacgat gtcgctcttt
    10861 atcaccgagc tggcctttgg aaccgatccg gccgcgaata atgcccggat cggcgtgatc
    10921 atcggctcga tcatctccgg cacgattggc tacctgatcc tgtcgcgcac gctgaaacat
    10981 cgatccaacg gagaggccgt aaattcaacg taggcagatt tgggcggatt ctgtcccgat
    11041 ccaccacaat tgcgatcgaa gttcgactac ctgatcggag gggctcgtgt ttcagcgtct
    11101 ggtcggaatt atctttggcg cgattgtcgc cttcctcctg ctcctgctct ttcacaacgg
    11161 ccggctcatc tcggacgagc gttctggttt cctgattgcg gtcgctgccg gcgcggtggc
    11221 aaatatgctg tggcccttga tctgggccgg tctcattgcg cgccgaaacc gtgtgaaccg
    11281 gcaggccacg gtccaggcag aggtccagcg ccaggtcaac acccaacgct cgaccgaacc
    11341 gcccacgcca ccgacggagt agcagctcct gcgcgagccg tgggccgtct ccgggagcta
    11401 ccggcaggcg ttgttgctcg cggtaggcat ggccaggaac gtgatgctga gcggcgcgag
    11461 cacgagcggt ccgggtcgcg acgtcgcgcc ggccagcggt ggcagggagt cgtccgggct
    11521 caattgcaaa tcggtcccgt tcaactggac gaccgagtcc tgcaaacccg tcgctgtcag
    11581 ggtgtagcgc tcgacgggca cggcgacctc cagggtgtgt gatgtcgccc ggtcggcgtt
    11641 gaccgccacc accgccacgc cgccctccgt ccctcgcagg ctatgggcga ggaggtacag
    11701 gttggctgcc ggcgacggcc cgggatcgag cacggtcgta cccatcaggt ctcgccagag
    11761 aagcgtcgcc caatagttgg gacgtggcgc gaaggtgttc tcgtccagca gtccgtaatc
    11821 gctggatgcg agcgtgttgt gcatgatgac ctgcaagccc cgccgggcga ggctgccgcg
    11881 ct
//
LOCUS       DATAOZ010000232        14151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6146825, whole genome shotgun sequence.
ACCESSION   DATAOZ010000232 DATAOZ010000000
VERSION     DATAOZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14151
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6146825"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1362)
                     /locus_tag="VHR64_06130"
     CDS             complement(94..1362)
                     /locus_tag="VHR64_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302443.1"
                     /translation="MVRFRWLAAALIAVLSLVGPASAQSPSPDLPALTIKPSDLDQDG
                     WLHQGAYVHSINDAARDRADYVGQGADTDEVVERLTSIGWQREYVSFLSHPSKSDPST
                     PDQIIRSYITEYDDVDGATAGFAYLEDESTVAAAKDVPLDHTYGQQSELTSERGFSSQ
                     TGRQFRSLDLTFRMGALIAGVNIIQYPTADRVDPDMTLLETLAGTMETRIAESPVIDS
                     ALGARIVRIAHDDTEVVTFDDAYYRIAGKDIPLEGESPDAAELRTDTYRDAVDVYQLW
                     QGVDTAEIAGALYGVTLLHFPDADAAAGWLTDLPEILATNPFYGDIGAVDAGVALGDQ
                     TVALSYASGGGGPDDPHALIIAVRIGPDVARLHLVPQGNQTSVPMPALAELAGAEADC
                     LATAGCTTTIPVPHSLLTTLTPANATPASE"
     gene            1579..2601
                     /locus_tag="VHR64_06135"
     CDS             1579..2601
                     /locus_tag="VHR64_06135"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX3302444.1"
                     /translation="MLAASVGWFVMRHGSEVAHGLAAIAQSDPKWALAVIAAQIGTIT
                     LITMKYRTLFGRLGVRVPPVAMARAYIRRHFIGIVLPFGGPVGLVQFVKDLGRQQVRS
                     ATVLTGSLMATIVNEISFFLFLIPVVAWLAIAGRVTSVMAVGLITLGTIVAVVLTFLL
                     TMIFRGSVPAWLSTRIPDRIMAGIDDIQSHQFGLRDIAAGIPFALGVNVAGFLMLAAS
                     LQAVGQHPSVTTLLVARVAASLAILALPVFQGAGAVEVTVIGALHAGGVPIGDAIAAT
                     LLFRIGQFWAPLAIGGLAILPWERCDPRSWQPIPVTAVRVAAAVAAVMLVVAPIGLLL
                     TVVDVT"
     gene            2643..4007
                     /locus_tag="VHR64_06140"
     CDS             2643..4007
                     /locus_tag="VHR64_06140"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302445.1"
                     /translation="MQSVRSFGQRTFASLSVRNYRLFFIGQGISQCGSWMQSIAQGLL
                     VLELTGSGTALGLVIALQNLPVLFFGPWGGVMADRFPKRTILYVTQALSGLLALTVGM
                     LVITDLVQLWMLYVLGLMLGFITVFDNPARQTFVREMVGNERLTNAVSLSSMEFNLAR
                     VIGPSIAGILAATVGLGACFIADGLSYIAVLTVLVMMRADELRPAVRVASGKGQLMNG
                     LRYVRTQPVLLNTLIMMAIVGMFTYEFSVMLPLFAEFTFDSGPGAYASMTAAMGAGAV
                     VGGLFAASRRRSTLGMSVAAGALFGASVMLTALMPTLHLAVLSLLVVGFFSITFTSLA
                     NVTLQLSSAPDMQGRVMSLWSMAFLGTTPIGGPVMGTIGEEAGARWALAIGGFAAIVA
                     AGIGFRAWLEARRREVAEERSELTVASGGLRERKMENGKPKTKDERPKTPLETRNEKL
                     ETPS"
     gene            4134..5081
                     /locus_tag="VHR64_06145"
     CDS             4134..5081
                     /locus_tag="VHR64_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEX3302446.1"
                     /translation="MELGGIHHLTAISARIRDNHHFYTQTMGMRLVKRSVNQDDVSAY
                     HLFYADAKGSPGTDLTFFDWPVPPERRGTRAIVRTALRVSGKETLDWWKQRLDEKGVT
                     AGDVVECDGRLTLDFEDDEGQRLALIDDGGADDAFPWDRSPVPAEHQIRGLGPITLSV
                     PLLEPTDALLQRGLQMRKVREYQDLRHSEQSVHVYEMGPGGPAAELHLAVQPDAPVAM
                     QGAGAVHHVAFRIPDDTYDDWANRLNDLGIPNSGKIDRFWFHSLYFREPNGILFEIAT
                     DGPGFGVDEDMATLGEKVVLAPFLEPRRDQIIANLKPID"
     gene            5102..5761
                     /locus_tag="VHR64_06150"
     CDS             5102..5761
                     /locus_tag="VHR64_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3302447.1"
                     /translation="MPRTELDFVHRYIPPNQDAEAVAGITLLALHGTGGDETDLIPLA
                     QAVLPGAGVLSPRGKVLEGNAPRFFRRLAEGVLDQEDLKFRTTELVRFVEAASAEYGF
                     DRGSVVAVGFSNGANIAASILLREPNVLRGAALLSPMLPFEPESTPDLSETDVFIGAG
                     RADPLVPVDQVKRLSDLLKTSGANVTLHWDQGGHTISPSELKAAQKWAMGLVEAADQF
                     R"
     gene            complement(5814..6728)
                     /locus_tag="VHR64_06155"
     CDS             complement(5814..6728)
                     /locus_tag="VHR64_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX3302448.1"
                     /translation="MRSTPGHGMMMNVEFDLAFIDMMTQHHEGAVAMAKVALERGEHE
                     EIRAFAQGIIDTQDAEIAHLQTWRAEWYPDAPPITMSDMMQMQGMGHMMPGMAELMQS
                     MGSMHVDSATGMAELCSVTDSFDRAFIDMMIPHHESAIMMARVALANAKHQELKDLAR
                     EIIDAQQREIDQMQGWRAAWYGAATPGTPAATLNSTTVTVTVDEFSVTASESEFQVGQ
                     TYRFVVTNNGALPHELMVLPEMAGIGEMDMEELHHVALGVIPVDDLAPGTTREIEETF
                     TEPGAYELACAVAGHYDAGMKLTVTAAK"
     gene            complement(7013..7462)
                     /locus_tag="VHR64_06160"
     CDS             complement(7013..7462)
                     /locus_tag="VHR64_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302449.1"
                     /translation="MTHTRRWTRPLIATWPLLVALLAFATISHDVAMAAHTHETPIPA
                     GVVEANIAHAFTGMHEGIPPETNLAAHLPSTDSTCPTDSCPDLTDCGLARVSNPIPAP
                     DLHPLATGLIDSASCLHSATGSDTPVSAIEPVHPPGVRRALLQVFLN"
     gene            complement(7559..9691)
                     /locus_tag="VHR64_06165"
     CDS             complement(7559..9691)
                     /locus_tag="VHR64_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX3302450.1"
                     /translation="MDHSAHTSRKQAPPEQPHHNHADMTHRGDLTDARDIDHQAIADS
                     GHAGHDAHPGHDKHAGHSVEMFRSRFWISLLLTIPVVLYSDMVQDWFNFSMPTFPGSG
                     AIAPVLGTVVFLYGGMVFLKGGLDEIRARTPGMMLLISLAISVAFLASAATTLGLFDL
                     DFWWELALLVTIMLLGHWQEMRAIGQAQGALSALAALLPDEAERVTDHGTETVPIAEL
                     HAGDIVLVRPGGRVPADGTIVEGQAELDESMITGESRPVERGTGDRIVAGSVVVGSAL
                     RGRVEATGDETALAGIQRLVAEAQASKSRAQALADRFAAALFFVAVGAGLITFLVWTL
                     LGDVEEAITRTVTVLVISCPHALGLAIPLVIAISTAISARQGILVKDRLALERMRLVD
                     TVLFDKTGTLTLGEHVVTDYAAVDVLEDDLLALAAAVESDSEHPLARAIVQAAQEKGI
                     RIPRATDFRASAGRGVEASVDGVNVAVGGPTMLRERKLEIPAALDGHEQAWKERGAAV
                     LHVARDGRIIGALALEDRVRPESRDAIDKLHQQGVRVVMITGDARSVAEGVAADLGVD
                     EVFAEVLPEDKESKVAELQQRGATVAMVGDGVNDAPALARADVGIAIGAGTDVAIESA
                     GVILATSDPRAVVGVRNLSKASYRKMVENLGWAAGYNIVAIPLAAGVLAPIGFTLAPA
                     VGAVLMSLSTIVVALNAQLLRRIDLRPQ"
     gene            9913..10362
                     /locus_tag="VHR64_06170"
     CDS             9913..10362
                     /locus_tag="VHR64_06170"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3302451.1"
                     /translation="MATIRIRSNRRLNASADRVYRCIADYRTHHPAFLPPAFSNFTVE
                     EGGVGSGTVIHFTLKAGGRTQTFRQRVSEPDPGRALTETTIGTQNSTTFTVTPDGSGS
                     NVSIMTEYEGSRGLSGMIERFLAPLLLRRLYKDELQRLDTYTQSTPI"
     gene            complement(10370..11314)
                     /locus_tag="VHR64_06175"
     CDS             complement(10370..11314)
                     /locus_tag="VHR64_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX3302452.1"
                     /translation="MSSEDHASTLAQLKDDFTRLPLKDLGKRAYKKIGADQVTTMSGA
                     FAYAWTFSIPPLILLTVLFAALLNEVTNVPVVENMQDAIKKHAPADTQEMLLRMVDNA
                     VAKVGGNTASVGVIVTAILALWAASSAIGVLITGFNRAYDVEEDRSFLKKKGVQLGLT
                     LMLVLFVNAVVALLVFGQQIGNWLADWIGVGSAFNTFWSFARWPLGILGIMLTLAVLY
                     WAGPNVDLPFRWVSAGTVVATVLWLALVAGFGLYMSVSNPGSAYGVVGAVIVLLVFLN
                     LTGIIFFLGAEINAILYSAVARHRVARWHEPGMAVANQ"
     gene            complement(11421..12431)
                     /locus_tag="VHR64_06180"
     CDS             complement(11421..12431)
                     /locus_tag="VHR64_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX3302453.1"
                     /translation="MPSSDLIVLIIIVALGLGFDFINGFHDTANAIATSVATRVLTPG
                     RAVLMAASMNVLGALTGTAVASTVGKGIIPPELATMELVAASLIAAITWNLITWWYGI
                     PSSSSHALVFSIVGAGIAAAGTEAIIFEGVSKVLKGMVYSPLLGFIGAFAIIFGLIWL
                     VARWRPRLVERVFGRAQILSAAFMAYSHGSNDAQKTMGVISLALASYYGWSGTEWHIP
                     LWVILSAATAMGLGTAVGGWRIVRTMGLKVVELRPIDGFAAETAAATVIEVASRLGIP
                     VSTTHVISSSILGVGATRRASAVRWGVAGRIVSAWVITVPICIAIGWLVFEIIDHIRG
                     WL"
     gene            complement(12424..13065)
                     /locus_tag="VHR64_06185"
     CDS             complement(12424..13065)
                     /locus_tag="VHR64_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX3302454.1"
                     /translation="MVLARFLPRDEQFFDYFNESAANALETAKLLTEIIEFGPDTERK
                     VRRLRDLEHTGDDITHRIYQALHSTFVTPLDRNDIRDLASILDDFVDGIEEVGKRILL
                     YQMGAPTEEARLFSRILVEQAETMAKGIPDLEQAGKNASQIQRCALELHRLENEADDA
                     LNQALAALYDGVTEVPQIIKALRWGEIYALLESATDKGENIGHTLEGILIKYA"
     gene            complement(13168..>14151)
                     /locus_tag="VHR64_06190"
     CDS             complement(13168..>14151)
                     /locus_tag="VHR64_06190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302455.1"
                     /translation="GSAFGTIGMAASAAAASGPPIGGLLVHWFGWQSIFWMNVPVVGL
                     AIALGLRSLPRSNAARENAHRAHFDIVGSALFTATLAALIVVPTMLKLSLPALGIALA
                     LGGLAIGAGFAFWELRTAAPVVDLRLFRTSAFAAACASVGISNLVMYTVLLAMPLYLE
                     RVRGHDSRVTGLTLAALSALSALAGPIGGRWMDTRGRVIPAVVGAVSLFAGIAGLTVA
                     IGGANLWPVAIALGFMGLGLGVAGASVQATAVEAVPVQRAGSASGIYSTARYLGSVIG
                     STALAVVFVSKPGAGESERFAALFAVLTVIAAAGIFVNTRLSLTGNGHMPE"
BASE COUNT         2419 a         4372 c         4862 g         2498 t
ORIGIN      
        1 tgctgcggcg cactgcgccg agagcacgta gtggggctcc gtacccgcct tgatcttctc
       61 cacaaacgca ctgaccgaaa ttgatgcctc gcgctactcg ctggccggtg tggcgttggc
      121 tggggtaagg gtagttagca ggctgtgggg aacggggatg gttgtggtgc agccggcggt
      181 ggcgaggcag tcggcttcgg cgccggccaa ctcggcaaga gcgggcatcg gcacggatgt
      241 ctgattacct tgggggacga ggtgaagacg agcgacatcc ggcccgatcc ggacggcgat
      301 gatcagggcg tgggggtcgt ctggcccgcc gccgccggag gcataggaaa gcgcgacggt
      361 ttggtcgccg agcgcgaccc cggcatctac cgctccgata tcaccgtaga acgggttggt
      421 ggcgaggatc tcgggcaagt cggttagcca acccgcggcc gcgtcggcgt cggggaagtg
      481 gagtagggta acgccgtaga gtgcaccagc aatctcggcc gtgtcgacgc cctgccagag
      541 ctggtagacg tcgaccgcgt cgcggtaggt gtcggtgcgc agctcggccg cgtcgggaga
      601 ttcgccctcg agcgggatgt ccttgcccgc gatccggtag taggcatcgt cgaaggtgac
      661 cacctcggtg tcatcgtgcg cgatccggac gatgcgggcg ccgagggccg agtcgatcac
      721 cggcgattcg gcgatccggg tttccatggt accggccagc gtttcgagca gggtcatgtc
      781 cgggtcgacg cggtcggcgg tcgggtactg gatgatgttg acgccggcga tcagcgcgcc
      841 catgcggaag gtgaggtcga gcgagcggaa ctggcgacca gtttggctgc tgaagccgcg
      901 ctcgctggtc agctcagatt gctggccgta ggtgtggtcg agtggcacgt ctttcgctgc
      961 cgccacggtc gactcgtcct cgaggtaggc gaatccggcc gtagcgccgt ccacgtcgtc
     1021 gtactcggtg atgtacgagc ggatgatctg atccggcgtc gatgggtcgg acttcgaggg
     1081 gtgggagagg aagctgacgt actcacgctg ccagccaatg ctggtcaggc gctcgacaac
     1141 ctcatcggta tcggcgccct gacccacgta gtcggcccga tcgcgtgcgg cgtcattgat
     1201 tgagtggaca tacgcgccct gatgcagcca gccgtcctga tcgagatcgg acggcttgat
     1261 tgtcagcgcg gggaggtcgg gcgaggggct ttgcgccgag gctggcccga ccaggctgag
     1321 tacggcgatg agcgctgcgg cgagccaacg gaaccggacc atacatatcc cctcgcgata
     1381 acttatcggc catattctat cgccaacgtc ctgagggccg ggcttcattt gtccttcatg
     1441 aacgagcggc attctggatg tggaagcgct ccggggagtg agcgccgaaa ctgaaagagc
     1501 agcctgccgt tgttccacgc ccttcttgct cgcgcccgaa catcaggctc acactacgca
     1561 accccgatct ggatactcgt gctggccgca tcagtcggct ggttcgtcat gcggcacggc
     1621 agtgaggtcg ctcatggcct cgccgcgatc gcacagagcg atccaaagtg ggcgctcgcg
     1681 gtgatcgcgg ctcagatcgg cacaatcacc ctgatcacga tgaagtaccg cacgctcttc
     1741 gggcgattgg gagtgcgcgt tccgccggtg gcgatggcgc gggcctatat ccgtcgccac
     1801 ttcatcggca tcgttctgcc gtttggcgga ccggtcggtc tggttcagtt cgtcaaggat
     1861 ctcggccggc agcaggtccg cagcgcaact gtgctgaccg gctcgctgat ggcgacgatt
     1921 gtcaacgaga tctcgttctt cctgttcctg atcccggtcg ttgcctggct cgcgatcgcc
     1981 gggcgcgtga cctcggtgat ggcggtcggc ctgatcacgc tcggcacgat cgtcgcggtg
     2041 gtactgacat tcctgctgac gatgatcttt cggggctcag tgccggcgtg gttgtccaca
     2101 cggatcccgg accgcatcat ggcgggcatc gacgacatcc agtcgcacca gttcggcctg
     2161 cgcgatatcg cggcggggat cccgttcgcg ctcggcgtca atgtcgcggg gttcctgatg
     2221 ctggcggcat cgctgcaggc ggtgggtcag cacccgagcg tgacgaccct gctggtggcg
     2281 cgcgtggccg cgtccctggc gattctggcg ctgcccgtct tccagggcgc cggcgcggtc
     2341 gaggtgacgg tgatcggcgc gctgcatgcc ggcggcgtgc cgatcggcga tgccatcgcg
     2401 gcgacgctgc tcttccggat cggccagttc tgggcgccgc tggcaatcgg cggcctggcg
     2461 atcctgccgt gggagcgctg cgatccgcga tcgtggcaac ccatcccggt gaccgcagtc
     2521 cgggtcgcgg cggccgtggc ggcggtgatg ctggtcgtcg cacctatcgg gttactgctg
     2581 acggtcgttg atgtgacgta ggggttacgg cggagcgcgt atcggtctgc gattattgcg
     2641 caatgcaatc tgtccgctcc ttcggccaac gcaccttcgc ttcgctctcc gttcgcaatt
     2701 accggctgtt cttcatcgga caaggcatct cgcagtgcgg gagctggatg cagagcatcg
     2761 cccagggatt gctcgtgctc gagctgactg gctcgggcac ggcgctcggc ctggtgatcg
     2821 ccctgcagaa tttgccggtg ctcttcttcg gcccgtgggg cggcgtcatg gcggaccgct
     2881 tcccgaagcg gaccatcctc tatgtgaccc aggcgctatc ggggctgctg gcgctgacgg
     2941 tcggaatgct cgtcatcacc gatctggtgc agctctggat gctctacgtg ctggggttga
     3001 tgctcggctt catcaccgtc ttcgacaatc cggcgcggca gaccttcgtg cgggagatgg
     3061 ttggcaacga gcgactgacc aatgccgtct cgctctcgtc gatggagttc aacctggcgc
     3121 gggtgatcgg cccgtcgatt gccggcatcc tggcggccac agtcggactg ggcgcctgct
     3181 tcattgcgga cggcctgtcc tacatcgcgg tgctgaccgt gctcgtgatg atgcgggccg
     3241 acgagctgcg gccggcggtt cgggtggcga gcggcaaggg acagctcatg aacgggctgc
     3301 ggtacgtgcg aacccagccc gtgctcctga acaccctgat catgatggcg atcgtcggta
     3361 tgttcacgta cgagttctcg gtgatgctgc cgctcttcgc ggagttcacc ttcgattccg
     3421 gaccgggcgc gtacgcctcg atgacggcgg cgatgggagc cggggcggtg gtcggcgggt
     3481 tgttcgcggc cagccggcgg cgatcgacgc tcgggatgtc ggtcgcggcc ggcgcgttgt
     3541 tcggcgcatc agtgatgttg accgcgctga tgccgacatt gcacctcgcg gtcttgtcgc
     3601 tgctggtggt cggcttcttc tcgatcacct tcacctcgct ggcgaacgtg acgcttcaac
     3661 tcagcagcgc gccggacatg cagggcaggg tgatgtcgct ctggtcgatg gcctttctcg
     3721 gcacgacgcc gatcggcggt cccgtcatgg gcacgatcgg cgaggaagcg ggcgcgcgct
     3781 gggcattggc catcggcggt ttcgcggcga tcgtggcggc gggcatcggg ttcagagcat
     3841 ggctggaggc gcgaaggcgc gaagtcgcgg aggagcggag tgagttgacg gttgcgagtg
     3901 gagggttgag ggagcggaaa atggaaaacg gaaaaccaaa gaccaaagac gaacgaccaa
     3961 agaccccgct agaaaccaga aacgagaaac tggaaactcc ttcgtaaccc tcaaccctca
     4021 acctgttctg tcgcctgctt gtgccgccgg ttaccatggg cggactagtg ttgcgcgcga
     4081 accgatcccg gatcgcgcgg gaacggaccc gcttggtgca gcccggaggc gacatggaac
     4141 tcggaggcat tcatcacctg accgcgatct cggccaggat tcgcgataac caccacttct
     4201 acacacagac catgggcatg cgcctcgtca agcggtcggt caaccaggac gatgtttcgg
     4261 cgtatcacct cttctatgcg gatgcgaagg gaagcccggg cacggacctg accttcttcg
     4321 actggccggt gccgcccgag cggcgcggaa ctcgcgcgat cgtgcggacc gcactgcggg
     4381 tctcggggaa ggaaacgctg gattggtgga aacagcggct cgacgagaag ggcgtcacag
     4441 ccggcgatgt cgtggagtgc gacgggcgat tgacgctcga tttcgaggat gacgaggggc
     4501 agcggttggc actgattgac gacggcggcg cggacgatgc ctttccgtgg gatcgcagcc
     4561 ctgtcccagc cgagcaccag atccgcgggc tcgggccgat caccctcagt gtgccgctgc
     4621 tcgagccaac cgacgccctg ctccagcgcg gcttgcagat gcgcaaggtg cgcgaatacc
     4681 aggatttgcg gcattccgag cagtcggttc atgtctacga gatggggccg ggcggtccgg
     4741 cggccgagct gcacctcgcg gtccagccgg atgcgccggt cgcaatgcag ggcgccgggg
     4801 cggtgcacca cgtggccttc cggattcccg acgacacata cgacgattgg gcgaaccggc
     4861 tgaacgacct cggcatcccg aacagcggca agatcgaccg cttctggttc cacagtctct
     4921 acttccgcga gccgaacggg atcctgttcg agattgcgac cgacggcccg gggttcggtg
     4981 tcgacgagga catggcgacg ctgggcgaga aggtcgtgct ggcgccgttc ctggagccgc
     5041 gccgggatca gatcattgcc aacctgaagc cgattgactg aagcaatttg aggtagccgc
     5101 catgcccagg accgaactgg acttcgtcca ccgctacatc ccgccgaacc aggatgcgga
     5161 agccgtggcc ggcatcacgc tgctggcgtt gcatgggacc ggcggcgatg agaccgatct
     5221 gattccgctc gcgcaggcgg tgctgcccgg ggccggcgtg ctcagtccgc gcgggaaggt
     5281 gctggagggg aacgccccgc gtttcttccg gcggctcgcc gaaggcgtgc tcgaccagga
     5341 ggacctcaag ttccggacca ccgagctggt ccggttcgtc gaggcggcct cggctgaata
     5401 cgggttcgac cgcggcagtg tcgtcgcggt cggcttctcg aacggcgcca acatcgccgc
     5461 gagcatcctc ctgcgcgagc ccaatgtcct gcgcggcgcg gcgttgctga gtccgatgct
     5521 gccgttcgag cccgagtcga cacctgatct gagcgagacg gatgttttca tcggcgcggg
     5581 cagggccgat ccgctcgtgc cggtcgatca ggtcaagcgg ctgtcggatc tgctcaaaac
     5641 cagtggtgcg aacgtgaccc tgcactggga ccagggcggc cacacgatct ccccgtccga
     5701 gctcaaggca gcccaaaagt gggcaatggg cctggtcgaa gctgccgatc agttccggta
     5761 gtcaggccca aatacgggtg gagcgggcca tgacccgctc cgatctagcg gagctacttt
     5821 gcggcagtca cggtcagctt catgccggcg tcgtagtgtc cggccacggc gcaggccagc
     5881 tcgtatgcgc ccggctcggt gaaggtctcc tcgatctccc tggtcgttcc gggggcgagg
     5941 tcatcgaccg ggatcacgcc gagagccacg tgatgcagct cttccatatc catctcgccg
     6001 atccccgcca tctccgggag caccatcagc tcgtgcggga gcgcgccgtt gttggtgacc
     6061 acgaagcggt aggtttggcc gacctggaat tctgactcgg acgcggtcac cgagaactca
     6121 tcgaccgtga cggtgaccgt ggtcgagttc aaggttgcgg cgggcgtccc cggagttgct
     6181 gcgccgtacc atgcggcccg ccagccctgc atctgatcga tctcgcgctg ctgggcgtcg
     6241 atgatctcgc gggccagatc cttgagctcc tggtgcttcg cgttcgcgag ggcgacccgg
     6301 gccatcatga tcgcgctctc gtggtgagga atcatcatgt cgatgaaggc gcggtcgaaa
     6361 gagtcggtga ctgagcagag ttcggccata ccggtcgcgg aatccacgtg catcgatccc
     6421 atgctctgca tcagctccgc catgccgggc atcatgtggc ccatgccttg catctgcatc
     6481 atgtcggaca tggtgatggg tggggcgtcg gggtaccact cggctcgcca cgtctggagg
     6541 tgcgcgatct ccgcatcctg ggtatcgatg atcccttggg cgaaggcacg gatctcctca
     6601 tgctcgcccc tctcaagggc gacctttgcc atggcgaccg caccttcatg gtgctgggtc
     6661 atcatgtcga tgaaggcaag atcgaactcc acgttcatca tcatgccgtg accgggcgtg
     6721 cttctcatgt ttccgctatc gtgcatgccg ttcatgcccg gtgccatctg gccaccggtc
     6781 gcagtggtca gttcggcgca tctgctgcct ggggcagggg ttgcattgtt cgccgttggc
     6841 gtgctagcga ggccgccggt cgccacaccg aacgcgattg ccattaccgc gacgactacg
     6901 gttccaagac gcaagaacaa tgttcctgat ttcatagtgt gattggcctc ctgaaagacg
     6961 cggcgaacca ccggagtgtg ccggtgggat cctgtgattc cggtcaggag gcctaattca
     7021 ggaagacctg gagcaaggcc cggcgaactc cgggcggatg aaccggctcg atcgcggaaa
     7081 ccggagtatc tgatccggtc gcgctatgga ggcaggatgc ggaatcgatg agtccggtcg
     7141 cgagggggtg gaggtccggt gcaggaatcg ggttactgac gcgggccagg ccgcaatcag
     7201 tcaagtcggg acaggagtcg gtcgggcatg tggaatctgt cgaaggcagg tgtgccgcca
     7261 gattcgtttc gggcggaatg ccttcgtgca ttcctgtgaa tgcgtgggcg atattggctt
     7321 cgacgacacc tgcggggatt ggggtctcgt gggtgtgcgc ggccatggcg acgtcatggc
     7381 tgatcgtggc gaaggcaagc aacgccacca ggagtggcca ggtcgcaatg agcggtcttg
     7441 tccaacggcg cgtatgtgtc atcagtcccc tcgagaccta ccggtgcgcg gccccggaca
     7501 cttcagtcta cgacggtccc gggccgccgg gatgaccccg gcgaccttga ttcgggcatt
     7561 actgcgggcg gaggtcgatg cggcggagga gttgggcgtt gagggcgacg acgattgtcg
     7621 agaggctcat caagacggcg ccgacggccg gcgccagagt gaatccgatc ggcgccagca
     7681 ccccggcggc gagcgggatc gcgacgatgt tgtagccggc ggcccagccc aggttctcga
     7741 ccatcttccg atacgaggcc ttcgaaaggt tgcgcacgcc gaccactgcc cgcggatcgc
     7801 tggtcgccag gatcaccccg gccgactcga tcgcgacgtc cgtgccggcg ccgatcgcga
     7861 tcccgacatc ggcccgggcc agggccggag cgtcgttgac accgtcgcca accatcgcca
     7921 ccgttgcgcc gcgctgctgt agctcggcga ccttgctctc cttgtcctcg ggcaggacct
     7981 ctgcgaagac ctcatccaca cccaggtccg cagcgacgcc ctcggcaacc gagcgggcat
     8041 cgccggtgat catgaccacg cgcacgccct gctgatgcag cttgtcgatc gcatcgcgcg
     8101 attccgggcg aacccgatcc tccagagcca gcgcaccgat gatccggccg tctcgtgcga
     8161 cgtgcagcac ggccgcgccg cgctctttcc acgcctgctc gtgaccgtcc agtgcggccg
     8221 ggatctcgag cttgcgctca cggagcatcg tcggcccgcc aacggcgaca ttgacgccgt
     8281 caacgctcgc ttcgactccg cgtcctgcgc tcgcccggaa gtcggtcgcc ctgggaatgc
     8341 ggatgccctt ctcctgcgcg gcctggacaa tcgcccgggc gagcggatgc tcgctgtcgg
     8401 actcgaccgc ggcggccagc gccagcagat cgtcttctaa gacatcgacg gcggcgtagt
     8461 cggtcacgac gtgctcgccg agagtcagcg tgccggtctt gtcgaagagg acggtgtcga
     8521 ccaggcgcat cctctcgagg gcgagccggt ccttgaccag gattccctgg cgggccgaga
     8581 tcgccgtcga gatggcgatt acgagcggga tagcgagtcc gagggcgtgc gggcaggaga
     8641 tgacgaggac ggtcacggtc cgggtgatcg cctcttcgac gtcgccgagc agcgtccaga
     8701 ccaggaacgt gatcaggccg gctccgaccg ccacgaagaa gagcgcggcg gcgaagcgat
     8761 cggccagggc ttgggcgcgg gatttcgacg cctgcgcctc cgcgaccagc cgctggatac
     8821 cggccagcgc cgtctcgtcg ccggttgctt cgacccggcc ccggagtgcg gagccgacca
     8881 cgaccgagcc cgccacgatc cggtcgccgg tcccgcgctc gaccgggcgg gattcgccgg
     8941 tgatcatcga ttcgtcgagc tcggcctgac cctcgacgat cgtgccgtcg gccgggacac
     9001 gtccgccggg acggaccagg acgatatcgc cggcgtggag ctcggcgatg gggaccgtct
     9061 cggtgccgtg atctgtgacg cgctcggcct catccggcag gagcgcggcg agggcggaca
     9121 acgcgccctg ggcctggccg atcgcgcgca tctcctgcca gtgaccgagc agcatgatcg
     9181 tgaccagcag agccagctcc caccagaaat cgagatcgaa gaggccaagc gtcgtcgcgg
     9241 cgctggccag gaacgcgacg ctgattgcca gggagatcag gagcatcata cccggtgtgc
     9301 gggcgcggat ctcgtccagg ccgcccttca gaaagaccat gccgccgtag agaaagacga
     9361 ccgtgccgag gaccggggcg atcgcgccgc tgccggggaa ggtgggcatc gagaagttga
     9421 accagtcctg gaccatgtca ctgtagagca cgaccggaat cgtgagcagg agcgagatcc
     9481 agaagcggct gcggaacatc tcgacgctgt ggcccgcgtg cttgtcatgt ccgggatggg
     9541 cgtcgtgccc ggcatggccg gagtccgcga ttgcctggtg atcgatgtcg cgggcgtcgg
     9601 tcagatcgcc ccgatgggtc atgtcggcgt ggttgtggtg tggttgctcg ggcggagcct
     9661 gcttgcggct agtgtgggcc gagtgatcca tggatttctc ctcgttatgg agccgccctg
     9721 gaattggggc aacgagttcc gagccggaag ttcatcaatt cgtggtaccc cgggcgggta
     9781 tgtcaattct cagtataccc gcccggggta tatggttcaa taacccttga aatcgtggcg
     9841 tggcttgtga gctttgcggg tttgcccagc ggcgcataat gcaggggagc gacaggaaga
     9901 ggagggtttt gcatggctac gatccggatc agatccaacc ggcggctgaa tgcgtcagcc
     9961 gatcgggtct atcgctgcat cgccgattac cggacgcatc acccggcgtt cctgccgccg
    10021 gcattctcga acttcacggt cgaggagggc ggggtcggtt ccgggaccgt gatccacttc
    10081 accctcaagg ccggcggccg tacgcagacg tttcggcagc gcgtatcgga gccggatccg
    10141 ggtcgcgccc tgaccgagac gacaatcggt acccaaaaca gcacaacctt taccgtcacg
    10201 cccgacggct ccggcagcaa cgtctcgatc atgaccgagt acgagggctc gcggggtctg
    10261 agcgggatga tcgagcggtt cctggctccg ctgctcctgc gccgcctcta caaggacgaa
    10321 ctgcagcggc tggacaccta cacgcagtcc accccgatct gacccggcct cactggttgg
    10381 cgaccgccat tcccggctca tgccagcggg cgacccgatg tcgtgccacc gcggagtaga
    10441 ggatcgcgtt aatctcggcc ccgaggaaga agatgatgcc ggtcaggttc agaaacacga
    10501 gcagcacgat cacggccccg accacgccgt aggcgctgcc cgggtttgag acgctcatat
    10561 agagcccgaa cccagccacg agggcgagcc agagcaccgt cgctacaacc gtgccggcgg
    10621 atacccagcg gaatggcaga tcaacgtttg gcccggccca gtacagcacg gccaacgtca
    10681 gcatgatccc gaggattccc agcggccagc gggcaaagct ccagaacgta ttgaatgctg
    10741 agccaactcc gatccaatcg gccagccaat tgccaatctg ctggccgaaa accagcaggg
    10801 cgaccacggc attcacgaag aggaccagca tgagcgtcag gccgagctgt acgcccttct
    10861 tcttgaggaa agatcgatcc tcctcaacgt cataggctcg attgaacccg gtgatcagga
    10921 cgccgatcgc gctcgaggcg gcccagagcg cgaggatagc ggtcacgatc acgcccacgc
    10981 tggcggtatt gccgccgacc ttggcgaccg cgttgtcgac catccggagc agcatctcct
    11041 gggtgtcggc gggcgcgtgc ttcttgatcg cgtcctgcat gttctcgacg acggggacgt
    11101 tcgttacctc gttcagcagc gctgcgaaca ggacggtcag cagaatcagt ggcgggatgg
    11161 agaaggtcca ggcgtaggcg aatgcaccgg acattgtcgt gacctggtcc gctccgatct
    11221 tcttgtacgc gcgcttgccg aggtccttca gcggcaaacg tgtaaagtca tccttcaact
    11281 gggcaagcgt cgatgcatga tcctctgatg acatgggggc tcctttccag gcatgcggag
    11341 gcggcgatgc tgtgctgccc caaggattga gcaaagccct cccactgtat gtagccggca
    11401 tccagatttc gtgtattggg ctacagccag cccctgatgt ggtcaatgat ctcgaagacc
    11461 agccagccga tcgcgatgca gatcggcacg gtgatgaccc aggcgctgac gatacgcccg
    11521 gccacacccc agcgcaccgc ggaggcccgg cgggtggcgc cgacaccgag gatcgacgac
    11581 gagatgacat gggtggtcga aaccgggatg ccgagccggc tcgcgacctc gatcacggtc
    11641 gcggcggcgg tctcggcggc gaagccgtca atcgggcgca gctcgacgac cttgaggccc
    11701 atggttcgca cgatgcgcca gccgccgact gcggtaccga gacccatcgc cgtcgcggcg
    11761 gagaggatga cccagagcgg gatatgccac tcggttccgg accagccgta gtagctggcg
    11821 agggcgagcg agattacacc cattgtcttc tgggcgtcat tgctgccatg gctgtaggcc
    11881 atgaaggcgg cggacaggat ctgcgcccgt ccaaagacgc gctcgaccag gcggggtcgc
    11941 caccgggcga ccagccagat caggccgaag atgatcgcga aggcaccaat aaagccgagc
    12001 aggggcgaat agaccatgcc cttgaggacc ttgctgacgc cctcgaagat gatcgcctcg
    12061 gtcccggccg cggcgatgcc ggcgcccacg atcgagaaga ccagcgcatg gctggagctc
    12121 gaagggatgc cgtaccacca cgtaatcagg ttccaggtga tcgccgcgat cagcgatgcg
    12181 gcgaccagtt ccatcgtcgc cagctcgggc ggaatgatgc ccttgccgac cgtcgaggcg
    12241 acggccgtgc cggtcagcgc gccaaggaca ttcatgctcg cggccatcag cacggcccgg
    12301 ccgggagtaa ggacccgggt tgccaccgag gtcgcgatcg cgttggcggt gtcgtggaag
    12361 ccgttgatga aatcgaagcc gaggccgagt gcaacgatga tgatcaggac gatcagatcg
    12421 gagctaggca tatttgatca ggatgccttc gagcgtgtgt ccgatgtttt cgcccttgtc
    12481 agtggcgctt tcgagaagcg cgtagatctc gccccagcgg agtgccttga tgatctgcgg
    12541 cacctcggtt acgccgtcgt agagagcagc gagggcctgg ttgagggcat cgtcggcctc
    12601 gttttcgagc cggtgaagct ccagggcgca gcgctggatt tggctggcat tcttgccagc
    12661 ctgctcgaga tcgggaatcc ccttggccat ggtttcggct tgttcgacca ggatgcggct
    12721 gaagaggcgg gcctcctcgg tgggtgcgcc catctgatag agcaaaatcc gtttgccgac
    12781 ctcctcgatg ccgtcgacga agtcatcgag gattgaggcc agatcgcgaa tgtcgttccg
    12841 gtcgagtgga gtgacgaagg tgctgtggag ggcttgatag atacggtggg tgatgtcatc
    12901 gccggtgtgc tcgaggtcgc gcaggcgacg gaccttacgc tcggtgtcgg ggccgaactc
    12961 gatgatttcg gtgaggagct tggcggtttc gagtgcgttg gcggcggatt cgttgaagta
    13021 gtcgaagaat tgctcatccc gcggcaggaa tcgcgccaaa accatcgcag tcgctcctcg
    13081 agaatggacc agggtcagac gctccgccca tcaaaatatt aacagtccgc gtgatcgggc
    13141 attgcaccgg gattagccgc ctggtaatca ctctggcatg tggccatttc cggtcaggct
    13201 gaggcgggtg ttgacgaaga ttccggcggc ggcgatgaca gtcaggacgg cgaagagagc
    13261 ggcaaaccgc tcgctttcgc ccgcgcccgg cttgctgacg aagacgacgg ccagcgcagt
    13321 cgagccgatc acgctgccca ggtagcgggc ggtcgagtag atcccggagg cggagccggc
    13381 gcgctggacc ggcacggctt cgaccgcggt cgcctggacc gacgccccgg ccacgccgag
    13441 accgagtccc atgaagccga gcgcgatcgc aaccggccag aggttcgcgc cgccgatggc
    13501 gacggtcagc ccggcgatac ccgcgaagag ggagaccgcg ccgactacgg cggggatcac
    13561 ccggccgcgc gtgtccatcc agcgcccgcc gattggacca gccagggccg agagagcgga
    13621 gagtgcggcg agggtcaggc cggtcacgcg ggagtcgtga ccgcggacac gctcgaggta
    13681 gagcggcatc gccagcagca cggtgtacat gaccaggttg ctgatcccga ccgaggcgca
    13741 agcggcggcg aaggcggagg tccggaagag gcggagatcg acgacagggg cggccgtccg
    13801 cagctcccag aatgcgaagc cggcgccgat cgccagcccg ccgagcgcga gggcaatgcc
    13861 gagggccggc agactgagct tgagcatcgt cggcacgacg atcagggcgg ccagtgttgc
    13921 agtgaacagg gccgagccga cgatgtcgaa atgggcgcga tgggcattct cgcgggcggc
    13981 attggatcgg ggcaggctgc gcaggccgag cgcgatcgcc agcccaacga ccggcacgtt
    14041 catccagaag atcgattgcc agccgaacca gtggaccagc agcccgccga tcggtgggcc
    14101 cgacgcggcg gccgcactcg cggccatgcc gatcgtgccg aatgcggatc c
//
LOCUS       DATAOZ010000233         6016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6675499, whole genome shotgun sequence.
ACCESSION   DATAOZ010000233 DATAOZ010000000
VERSION     DATAOZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6675499"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..535
                     /locus_tag="VHR64_06195"
     CDS             68..535
                     /locus_tag="VHR64_06195"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX3302456.1"
                     /translation="MFDEVKGFAVTFKRLFVPKITIQYPEEKRDMAPRFRGLPSLRVD
                     PESGEALCVACGLCARICPTSCLEMHVVPSEEGDRELGEFILKAGRRMFCGLCSQVCP
                     VDAITMSGEYELSVMERDGLVYTKTELATIGYDQDTWWESGLTDRIPKVLESQ"
     gene            complement(584..1441)
                     /locus_tag="VHR64_06200"
     CDS             complement(584..1441)
                     /locus_tag="VHR64_06200"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX3302457.1"
                     /translation="MTIELGAHVSASGGVDLALVRANDFGMTACQLFTKSERQWAAKP
                     LDPAVVERFHANQEQFRLKHLVAHDSYLINVASPNPEMWEKSRSALAHELERCDTLGI
                     HYLVSHPGAHMGEGPEAGIARVAEAINRIHDEMPDGKAKILIETTAGQGTALGRSFEE
                     IAGMIAGVDAKDRIGVCLDTCHIFAAGYDIRDAESYSATIQAFEDIVGLSNLKCLHLN
                     DSKKGLGLHVDRHAHIGEGEIGLAGFACLMNDKRLCGLPGILETPKEAPDFEEDRMNL
                     NALKALIAA"
     gene            complement(1447..1815)
                     /locus_tag="VHR64_06205"
     CDS             complement(1447..1815)
                     /locus_tag="VHR64_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEX3302458.1"
                     /translation="MSRVRIYLYSNCSSCKNAEQVLSDAGVEYDRRDIFRERMTVPEL
                     EALFHEIGKSPTELLSRRSIPYRQLGLAERAPSDSELIELMTEHPGLLRRPIVIAAGE
                     VQIGFNRAALESLAQRNAQD"
     gene            1974..2780
                     /locus_tag="VHR64_06210"
     CDS             1974..2780
                     /locus_tag="VHR64_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEX3302459.1"
                     /translation="MVAAEIRDYFVREPVLELVPRPRLSLIIPAFNEQSRIERTLADS
                     WDYLTAQPYSFEIILSDDGSTDRTLAIGQSFEAAHENVCVLSITHGGKAAAIRAGMQA
                     ARGEIVVFTDADLATPISYLGDFMAAIERGADIVIGSREGAGANRVGEPIYRHLMGRA
                     FNRLVQTLMLPGIDDTQCGFKAFTRFASQEITRRAKLYADHEVITGARVTAFDVEMLV
                     IARKLGLKIVEIPVVWTYGTQSKVNPVRDTLINLRDILTIKFNAVRNAYD"
     gene            2945..3646
                     /locus_tag="VHR64_06215"
     CDS             2945..3646
                     /locus_tag="VHR64_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX3302460.1"
                     /translation="MREFPLAEILQFISLGSRSGILEIVRRDGVYGVIFTSGTITGLT
                     ADGWTLANELRESQLLPDNILTGLLAVNSNIQDLRAAILTGGYMTADEWTAFVSRQVE
                     RLLYSLFDVRDGKFRFRQSAPPEGQWLPVRVSADRAVLEGTRWSEAWARASEVIPSRQ
                     SRFARTGRVPRANIRVTPTQWRVFVSTNEPGTLNQIAARSILSEVDVIESLQYLLDLN
                     LVARLDATGIAANGR"
     gene            3868..4932
                     /locus_tag="VHR64_06220"
     CDS             3868..4932
                     /locus_tag="VHR64_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX3302461.1"
                     /translation="MQSTLRELIGILGDAEIVARDALIRFNDLVARAEAVNRSAANER
                     AELEQFLEQFVLEAGQSAGSGGNRTFHGEFVNLEAMRADVVNIALNASKAAILTSSLT
                     SIRNSVENAARHFGDDSALTVSLDATSEHFQTAINEAREDERRKLAREIHDGPAQVLA
                     NAIYAVQIVEQLVKRNPDAVEEEIGRVRDLLKEGVTEVRRFMFDLRPATLQDYGLAQT
                     VQKYVEDYGRFFGRPATCSVTGTMPPLTPEQDLTVFRIIQEALQNAHKHAGNDAAVEV
                     TMSADASNLTVRIVDDGVGFDPALVSPKLNSGAGLLGMRERASIARARLTIESRPGGG
                     SVVTLTLPIDAQSDLGQLRS"
     gene            4956..5660
                     /locus_tag="VHR64_06225"
     CDS             4956..5660
                     /locus_tag="VHR64_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3302462.1"
                     /translation="MNRIQVLIVDDHPLFRRGVRWGLEAEPDILVVGEATDGQMAVQQ
                     ADLLVPDVVLIDINMPQMNGLEVTRIIRRRHPQTGVIVLTMHEDDEQLFHAIRVGAAA
                     YCTKDAQQDEIIKLIRRVARGEYLINENVLQRPFVASRVLDQFRELSQMESGAEGVFS
                     PLTPREVEILDCVAQGNSNKEIARILNISDQTVKNHITSILRKLAVNDRTQAVIYALR
                     HGWIRLTDDSPATMNG"
     gene            complement(5726..>6016)
                     /locus_tag="VHR64_06230"
     CDS             complement(5726..>6016)
                     /locus_tag="VHR64_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEX3302463.1"
                     /translation="LRAIPAIMTGVDIRERIHLILRELAEGGVGESWLDSVAISAACH
                     TSIRAGQVLSISEMRELVNQLEQTEHPRACGHGRPTMLLMSQGDLEKQFSRR"
BASE COUNT         1154 a         1893 c         1807 g         1162 t
ORIGIN      
        1 gtggtcccga cggtcgggca acgttgtcgc tcgcgtacac cgcggcggtc taaggagcac
       61 ctgacgcatg ttcgacgaag tcaaggggtt cgccgtcacc ttcaagcggc tgttcgtccc
      121 caagatcacg atccagtacc ctgaggagaa gcgggacatg gcgccgcgct tccgcggact
      181 gccatcactt cgggttgatc cggagtccgg agaggcgctc tgcgtggcgt gcggtctctg
      241 cgcgcggatt tgcccgacct cctgcctcga gatgcacgtc gtgccgtcgg aggaaggcga
      301 ccgcgagctt ggcgaattta tcctgaaggc cggccgccgc atgttctgcg gtctctgctc
      361 gcaggtttgc ccggtcgacg cgattaccat gtccggcgaa tacgagctct ccgtgatgga
      421 acgcgacggt ctcgtctaca ccaagacaga gctggcaacg atcgggtacg accaggacac
      481 ctggtgggaa tccgggttga ccgaccgaat tccaaaggtt ctcgaatccc aataggctcg
      541 gcaagagagg cgggagcgag cacgaactcg ctcccgcctt tatttatgcg gcgatcagtg
      601 ccttgagcgc attcaggttc atccggtcct cttcgaaatc cggcgcctcc ttcggcgtct
      661 cgaggattcc cggcagcccg cagaggcgct tgtcgttcat caagcacgcg aatccggcca
      721 gtccgatctc gccctcgccg atgtgcgcgt gacggtcgac gtgcaggccc agacccttct
      781 tcgagtcgtt gaggtgcagg cacttcaggt tgctcagacc gacgatgtct tcgaacgcct
      841 ggatagttgc ggaataactc tcggcgtccc ggatgtcgta cccggcggcg aagatatggc
      901 atgtgtcgag gcagacgccg atccgatcct tcgcgtcgac cccggcaatc atcccggcga
      961 tctcctcgaa cgatctgccc agtgccgtgc cctgtccggc agtcgtctcg atcaggatct
     1021 tcgccttgcc gtccggcatc tcatcgtgga tccggttgat tgcctcggca acccgcgcga
     1081 tgcccgcctc aggtccctcg cccatatgcg caccggggtg cgagacgaga tagtgaattc
     1141 cgagcgtgtc gcaccgctcc agctcatggg ccagcgcact ccgcgacttc tcccacattt
     1201 ccggattggg cgatgcaacg ttgattagat acgagtcgtg cgcgaccagg tgcttgagcc
     1261 tgaattgctc ctggttcgcg tggaatcgtt cgaccacggc gggatcaagc ggctttgctg
     1321 cccactgccg ctccgatttc gtgaaaagct ggcaggcggt catcccgaaa tcgttggcgc
     1381 gaaccagcgc aagatcgaca ccgccggatg cggatacgtg ggctccaagc tcgatcgtca
     1441 tcaacctcaa tcctgtgcgt ttcgctgcgc cagcgattcc agcgccgctc gattaaaccc
     1501 gatttgaacc tctcccgcgg cgattacgat tggtcgccgg agtaatcccg ggtgctcagt
     1561 catcaactcg attaactctg agtcagaggg cgcccgctcg gccagcccga gttgccggta
     1621 cgggatgctc cgtcgtgaca acaactccgt cggtgacttg ccgatctcat ggaacagcgc
     1681 ctccaactcg gggacggtca tgcgctccct gaaaatatcc cgacggtcgt actccacccc
     1741 ggcgtccgaa agcacctgct ccgcgttctt gcacgacgag caattgctgt acaggtagat
     1801 acgaacccgt gacattggta tatcccagcc caccaaaatt gtgacctctg gcagcccaat
     1861 ggctggacca aagcaggatc acgacagatt gccacgagcg cggcttcatc taggctacac
     1921 tcggtggcac cgcgggcgcc acacatgcaa tgctgagcgg agcgagacac gctgtggtcg
     1981 ctgcggaaat tcgagactac ttcgtccggg agcctgttct ggaactcgta ccgagaccac
     2041 gactctcgct catcattccc gcattcaatg agcagtcgcg catcgagcga actctcgcgg
     2101 attcgtggga ttacctcacc gcgcagccat actcgttcga aatcatcctc agcgatgacg
     2161 gcagcaccga tcgaacactg gccatcggtc agtcgttcga ggctgcccac gagaatgtct
     2221 gcgtcctctc gatcacgcac ggcggcaagg cggccgcgat ccgagccggc atgcaagcag
     2281 cccgtggcga gatcgtggtc ttcacggatg ctgacctggc cacgcccatc tcctacctcg
     2341 gcgacttcat ggccgcgatc gagcgcggcg ccgacatcgt gattggatcg cgtgagggcg
     2401 ccggagccaa ccgcgtcgga gagccgatat atcgacatct gatggggcgg gccttcaatc
     2461 ggttagttca gacgctgatg ctgccgggaa tcgacgacac gcagtgcggc ttcaaggcat
     2521 tcacgcgctt cgccagtcag gagatcaccc gccgcgcaaa gctctatgcg gatcacgagg
     2581 tgatcaccgg cgcgagggtg accgcattcg atgttgagat gctggtcatc gctcgcaaac
     2641 ttggcctcaa gatcgtcgaa attccggtcg tttggaccta cggcacgcag agcaaggtca
     2701 acccggttcg ggatacgctg atcaacctgc gggacattct tacgatcaag ttcaacgccg
     2761 tccgaaacgc ctacgactag cctcccatcc ggcggctttc cgggagtcct gttttcggtc
     2821 cgggcgcgtg tgaccaaaag gttatggagc gtcggctacc gtgccggtag gcttgacctg
     2881 accggtcaca accggctctg gacgttcgtc tgatcgccgt gggattgtta ttgtggaagg
     2941 cgacatgcgg gagttcccgc tcgcagagat cttgcagttc atctccctcg gctcccgatc
     3001 aggcatcctc gagatcgtgc gtcgggatgg cgtatacgga gtgatcttca catccgggac
     3061 gattaccggc ctgaccgcgg acggctggac cctggcgaat gagctgcgcg agtcccaact
     3121 ccttcccgac aacatcctga caggcctact cgcggtcaac agcaacatcc aggatttgcg
     3181 cgccgcgatt ctgaccggcg gatacatgac cgcagatgaa tggaccgcct ttgtctcgcg
     3241 ccaggtcgag cgactgctct attcgctctt tgatgtccgc gacggcaagt tccgtttccg
     3301 ccagtcggcg ccgcctgagg gccagtggct accggtgcgc gtctcagccg atcgagctgt
     3361 ccttgagggc acccgatgga gcgaagcctg ggctcgggca tcggaagtca tcccgtcccg
     3421 ccagagccgc ttcgcacgga ccggtcgcgt cccccgtgca aatatccgcg taacgccgac
     3481 gcagtggcgc gtctttgtct cgaccaacga gccgggcaca ctgaaccaga tcgcggcccg
     3541 ctcgatcctc tccgaggtcg atgtgatcga gtcgctgcaa tatctcctgg acctgaacct
     3601 cgtcgctcgg ctcgatgcga ccggaattgc cgcaaacggc aggtagaacc ccctcgacaa
     3661 ttttgcgcaa gtacggtact gtacgtacgc atagttcgtt ctacttacga acatgggata
     3721 cgggactttt tggtgacaaa tcggcgtgaa cggccggcag tccgaaggta ccgtttctct
     3781 atacccgctc tagcggtcct cccgtagcga ccgtcgtgaa cagcgttggg ttccatgggg
     3841 acacgctcaa acgtgacgtc ggctgaaatg caatcaaccc ttcgggaact tatcggaatc
     3901 ctcggcgacg cggagattgt cgcccgggat gctctaatcc gtttcaacga cctggttgcg
     3961 cgggccgagg ctgtcaatcg cagcgcggcg aacgaacggg cggagcttga gcaatttctc
     4021 gagcagttcg tgctcgaagc cggacaaagc gcgggctcag gcgggaaccg caccttccat
     4081 ggcgaattcg tcaatctgga agcgatgcgg gctgatgtcg tgaatatcgc gctcaatgcc
     4141 tcgaaggcgg ccatcctgac cagcagtctg acctccattc gcaactcggt cgaaaatgcc
     4201 gcccgccatt ttggcgacga ttcggccctg acggtctcgc tcgacgcgac gtccgagcac
     4261 ttccagacag cgatcaacga ggcccgcgag gacgagcgcc gcaaacttgc ccgagagatc
     4321 catgacggcc cagcccaggt gctggccaac gcgatttacg ccgtccagat cgtggagcag
     4381 ctggtcaagc ggaacccgga cgcggtcgaa gaagagatcg ggcgggttcg cgatctgctc
     4441 aaggaaggcg taactgaggt tcggcgcttc atgttcgacc tccggccggc caccctgcag
     4501 gattacggtc tggcccaaac cgtccaaaaa tatgtcgagg attacggccg cttctttggt
     4561 cgacctgcga cctgctcggt gaccggcacg atgcctcccc tgacaccgga gcaggatctg
     4621 accgtattcc ggatcatcca ggaggcactc caaaacgccc acaaacacgc cggcaacgac
     4681 gcggcggtcg aagtcacgat gtcggcagac gcgagcaacc tcactgtccg gatcgtcgat
     4741 gacggcgtcg gcttcgatcc ggccctggta tcgccgaagc tcaactcggg agccggcctg
     4801 ctcggaatgc gcgagcgcgc atcgatcgcg cgggctcgtc tcacgatcga gagtcgcccc
     4861 ggcggtggat cagtcgtaac gctcaccctt cccattgatg cccagtcaga cctgggacag
     4921 ttgcgctcat gatggatgag ccaaggagga catcagtgaa tcgtatccag gtcttgatcg
     4981 tcgacgatca tcctctcttc aggcgcggcg tgcgctgggg cctcgaggcc gagccggaca
     5041 tcctcgtggt cggtgaagcg accgatggcc agatggctgt gcagcaggcc gatctgctgg
     5101 tccccgatgt cgtgctgatc gatatcaaca tgccgcagat gaacggcctg gaagtgacgc
     5161 ggattatccg gcggcgccat ccccagaccg gcgtgatcgt cctgacgatg cacgaggacg
     5221 atgagcagct cttccacgcg atccgggtgg gagcggccgc ctactgcaca aaggacgccc
     5281 agcaggacga gattatcaag ctcattcgaa gggttgcccg gggcgagtac ctgatcaacg
     5341 agaacgtcct ccagcggccg ttcgtcgcgt cgcgagtgct cgaccagttc cgcgagctct
     5401 cgcagatgga gagtggagcc gagggagtct tctcaccgtt gacgccgcgc gaggtcgaaa
     5461 tcctggactg cgttgcccag ggcaacagca acaaggagat cgcgcgaatc ctcaacatct
     5521 cggaccagac ggtcaagaac cacattacgt cgatcctgcg caagctggcg gtgaacgacc
     5581 ggacacaggc ggtgatctac gccctgcgcc acggctggat ccggttgacc gacgactcac
     5641 ctgcgacgat gaacggctag atagtctttt cctgagattg gctgggggag acggatcggc
     5701 gcagtgccgg tccatttctc gctccttagc ggcgcgagaa ctgcttctcg aggtcgccct
     5761 ggctcatcag aagcattgtc gggcgaccgt gtccacacgc gcgtgggtgc tcagtctgct
     5821 cgagctggtt gactagctcc cgcatctcgg agatcgacag gacctgcccg gcccgaattg
     5881 acgtatggca ggcggcgcta atcgcgaccg aatcgagcca ggattctccg acaccgcctt
     5941 cggccaactc gcgcaggatg aggtgtattc gttcccgtat gtcgaccccg gtcatgatcg
     6001 ccgggatggc tcgaag
//
LOCUS       DATAOZ010000234         6223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2992704, whole genome shotgun sequence.
ACCESSION   DATAOZ010000234 DATAOZ010000000
VERSION     DATAOZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2992704"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VHR64_06235"
     CDS             complement(<1..187)
                     /locus_tag="VHR64_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302464.1"
                     /translation="MRRWVGALICVLLTVATISAASADSPATPSPAATPFDCPVTQPN
                     GIQPPPGANVFGKGNGDY"
     gene            complement(286..846)
                     /locus_tag="VHR64_06240"
     CDS             complement(286..846)
                     /locus_tag="VHR64_06240"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX3302465.1"
                     /translation="MMLMGDTPRKWWVYVVRGIAAIAFGIAAWAWPNLTIGTLVILFG
                     IFAIIDGILSIWAALLTNAEGHRLSLILAGLAGIAVGVISIAWPELTATTVVFLIAIW
                     AISVGLMQIFSAWQLRKEIENEWLLIIAGAGGVIWGLLVMVFPGGGAVSIVWAIGLFA
                     ILLGCLLIALGLRVRKWGGPGTPVPA"
     gene            complement(1034..1363)
                     /locus_tag="VHR64_06245"
     CDS             complement(1034..1363)
                     /locus_tag="VHR64_06245"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX3302466.1"
                     /translation="MAFDEGLADRIREVLSGEPGLTAKKMFGGYGFLLDGNMCAGVQG
                     DRLIVRVPADEHEAILAQPDVDQFPSPDRPMKGWVTVGPDAVAEDEDLARWVQMGVAI
                     ARSLPPK"
     gene            complement(1365..2561)
                     /locus_tag="VHR64_06250"
     CDS             complement(1365..2561)
                     /locus_tag="VHR64_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3302467.1"
                     /translation="MAALVDRVRGDIDEIMPGVIADRRQFHENPELGFQEFETARIVA
                     ERLRSIGVEDVRTGIAVTGVTGLIHGGKGPGKVVLLRADMDALPILEENDVPYRSKVD
                     GKMHACGHDAHTAMLLGIARVLIDRKDEFAGTIKVLFQPAEEGGGGAKVMIDEGVLSD
                     PDVDAVFGMHMDQGHALGTISTRPGPAMAAADRVYVTIKGKGGHGAHPDLTVDPIAVG
                     AQIVTALQTIVAREIDPVKPAVCTVGALIAGEAANVIPDTAIMRGTLRSFDPEVRTHL
                     GEAVERVVRGIAAAMRAEVEYDYAQGYPATVNEPEMTRLVMDVAAEVVGAENVIEAPM
                     MMGAEDFSYFLEQKPGCFYFVGSANEERGLGWGHHHPRFDIDEESMANGMEVMVGTVL
                     RYLESA"
     gene            2693..2857
                     /locus_tag="VHR64_06255"
     CDS             2693..2857
                     /locus_tag="VHR64_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302468.1"
                     /translation="MNRRTRSISILAGGIIGAAVGIAFGYVPFGTGISIVIGMATGAV
                     LSMVFASIRR"
     gene            3078..4568
                     /locus_tag="VHR64_06260"
     CDS             3078..4568
                     /locus_tag="VHR64_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302469.1"
                     /translation="MFMPACHIGRPPAARLTAILGALAVLLALAAPASASGWGGNSGP
                     DTWPVAEGGSFRYHVQPGATGAQMPSAVGFRTEFGPVFDSAETLASSILATSKISPVD
                     VYVFANLGLFEATVKARPVGLTPHSTVVYDPAAGEVLVNLTDVLALSPTQVQDAIGNA
                     VTQLAVQHASSGKAPAGIAAGIALYVELPTSEYLARIASTVQASYQKNTLLTWFDLNR
                     PIAAKDQELALGECYAMASFLIDRYDIPALRTMLGDLPGAAQWQDAYRSAFAADPSVI
                     EQQWRADLPRWTTSGWRDNLIASFDLEPARTMLGQGQYVAAKALLDPSLNLYRQLNDP
                     ESLAQTQTLMNQADTGIQAEALMVEIEKALQDHDYARASNLLDQADIQYKALPTEQVP
                     QSLLSTYRQMATDGQTATAQLAEADRLARSWGSYPEARAAARDAGATFARLGDEQNRQ
                     SAEQVLNKLDNRQRRLVVMMIGLGVITLVWLMLWLRARGPSDVRWA"
     gene            4571..>6223
                     /locus_tag="VHR64_06265"
     CDS             4571..>6223
                     /locus_tag="VHR64_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302470.1"
                     /translation="MATRVLTTGRTIATTWERLAQTLATWSPMAMLLQLRNFLWPTSA
                     SLRERWMRLVWLPFWIGTEIAAPHTGRQARVRGDLGPASRRLVMYLTSIRRRQLVIRY
                     ATVIARGIALGLLVCAIWAAWAASGGRQINSEALLITTGILVAIGLVFGWRHRPDLRE
                     TATMLDNSFALSDRTATAFDHLTIGPNRIQGQPHLAYLQIAEATNTVVMLNKHSALRV
                     RPPVRELVLIAGLALLFLGLYLVRGTGAGLPEAGNSSVPAFVAAKDRLQQQPTIPTPP
                     AEVNKAPSVEDVQKRADQSSATEQDLLKIADALADNPLTAPIADSIKAGDYQKAADQL
                     NAVSSQVSQLSPESRADLADKLDQAADGMTGQHPDLQDATHKAADGLRSDPQQAQSSL
                     SNLSDQISKTGQTVESQQALAKDMRDAREAQASGSSNQGSRPEPGDTKADSSQGNQQI
                     AGQGGDASSGQGQQPDQGQQTNGSGQSDSNKGNTQSQSDQGGSSSDSSSAPPSGQQNG
                     ASGQSTGNGPAGQVDPSSGNGSGQTGTGETSGPSQPSQGTTGG"
BASE COUNT         1114 a         2052 c         2009 g         1048 t
ORIGIN      
        1 cataatcacc atttcccttg ccgaagacgt tcgcgccggg cggcggctgg atgccattgg
       61 gctgcgtgac ggggcaatca aatggagtgg cagccggcga tggtgtcgcg ggtgagtccg
      121 cactcgcggc ggagatcgtc gctacagtca gcagcacgca gatcagcgca ccaacccatc
      181 gtctcatcgc gcgccctccg gtatcgatca acatcgtcta ccgaaagtac aacgccggat
      241 gtcggttggt tcccgatccg actggcgtga cttgccagac ccggttcagg caggcaccgg
      301 agtgccgggg ccgccccact tgcggacgcg caggccgagg gcgatcagga ggcagccgag
      361 caggatcgcg aacaggccga tggcccagac gatcgagacc gccccgccgc ccggaaagac
      421 catgacgagc aggccccaga tcaccccacc ggcgccggca atgatcagga gccattcgtt
      481 ttcgatctcc ttgcgcagct gccaggcgct gaagatctgc atcaggccga ccgagatggc
      541 ccagatggcg atcaggaaga cgaccgtcgt ggcggtcagc tccggccagg cgatgctgat
      601 cacgccgacg gcgatgccgg ccaaaccggc gaggatgagc gagagccgat gcccctcggc
      661 gttggtgagg agggccgccc aaatcgagag gatgccatca atgatcgcga agatgccgaa
      721 gaggataacg agggtgccga tggtcaggtt gggccacgcc caggccgcga tcccgaacgc
      781 gatagcggcg atcccccgaa cgacgtagac ccaccatttc cgtggcgtgt ctcccatcaa
      841 catcatcgct ctcctcctcc gaggttgcga acgggtcgca tcgaatcccg acggatgggc
      901 ttcaaagtcg catggcgaaa tgccggaata gattcctgcc tctcactata tgaggggagc
      961 gcaagcaacc atgcctgcac ccctggccgc ccaagggtgc aaaacagtcg caggattcag
     1021 ggagtgagcg ggtctacttg ggaggcaggg aacgggcgat ggcgacgccc atttggaccc
     1081 agcgggcgag atcctcgtcc tcggcgacgg cgtccgggcc gaccgtgacc cagcccttca
     1141 tcgggcgatc gggactgggg aactgatcga cgtccggctg cgcgaggatc gcctcatgct
     1201 cgtccgccgg cacccggaca atcaggcgat cgccctggac gccggcgcac atgttgccgt
     1261 cgagcaggaa gccatacccg ccgaacatct ttttggcggt caggccgggc tcgccggaaa
     1321 ggacctcgcg gatccggtcg gcaagccctt catcgaaggc catctcaggc ggactcgagg
     1381 taacggagca cggtcccgac catgacctcc atgccgtttg ccatcgattc ttcgtcgatg
     1441 tcgaagcgcg gatggtggtg gccccagccc agacctcgtt cctcgttggc gctgccgaca
     1501 aaatagaagc agcccggctt ctgctcgagg aaatagctga agtcctcagc gcccatcatc
     1561 atcggcgcct cgatcacgtt ctccgcgccg acgacctcgg cggcgacgtc catcaccagg
     1621 cgggtcatct ccggctcatt gacggtcgcc gggtagccct gcgcgtagtc gtactcgacc
     1681 tcggcgcgca ttgcggcagc aattccccgg acaacccgct cgactgcctc gccgaggtgc
     1741 gtgcgcactt cggggtcgaa ggagcggagc gtgccgcgca tgattgcggt atccgggatg
     1801 acgttggcgg cctcgccggc gatcagcgca ccaaccgtgc atacggccgg tttgaccggg
     1861 tcgatctcac gggcgacgat cgtctggagc gcggtcacga tctgggcgcc gaccgcgatc
     1921 gggtcgacgg tcaggtccgg gtgcgcgccg tgcccgccct tgcccttgat cgtgacgtag
     1981 acgcggtcgg cggcggccat cgccggaccg ggccgggtcg aaatcgtgcc gagcgcgtgg
     2041 ccctggtcca tgtgcatgcc gaagacagca tcgacgtccg ggtcggagag aacgccctcg
     2101 tcgatcatga ctttggcgcc tccgccgccc tcttcggccg gctggaaaag gaccttgatg
     2161 gtcccggcga actcgtcctt acgatcgatc aggacgcggg cgatgccgag aagcatggcg
     2221 gtgtgggcgt cgtggccgca ggcgtgcatc ttgccgtcga ccttcgagcg gtacggcacg
     2281 tcgttctctt cgaggatcgg cagggcgtcc atgtcggccc ggaggaggac gaccttgccc
     2341 gggcccttgc cgccgtggat caggcccgtc acgccggtga ccgcgatacc ggtgcggaca
     2401 tcctcgacgc caatcgatcg caggcgctcg gcgacgatcc tggccgtctc gaattcctgg
     2461 aacccgagct ccggattctc gtgaaactgc cgcctgtccg cgatcacgcc tggcatgatc
     2521 tcatcgatat cgccacgcac ccgatcaact aatgctgcca cgcttgaatt cctcctcacc
     2581 ggtgatgaac gagcgtcgtg ctcgcactgg atggcggtga ttgtacgggg tcctgctcac
     2641 ctatgatgag aatgctcggg aacccattcg cacggtaagg cgacacctga cgatgaaccg
     2701 gcgaactcgc tcaatcagca tcctggccgg gggcatcatc ggcgcggcgg tcggcatcgc
     2761 gttcggctat gttccgttcg gaaccgggat cagcattgtg atcgggatgg cgacgggcgc
     2821 ggtgctctcc atggtgttcg catccatcag gaggtaagtc cggcgcgtgg ctcccagagc
     2881 gacgttgtgc taggctgacc gttccattcc gggaagggaa atggctggtg ggcctgtcat
     2941 gtgcgcatgc ggtgcattgg catcgcgtaa ccggttatag tgcgtccatt ggttgacgaa
     3001 tcacgataat agatctgtac ccgatgcgcc gatgaatctc gccgcatcgt tgagagcgat
     3061 acaggagaag cggtgcgatg tttatgcccg cttgccacat aggccgacca ccggcggcca
     3121 gactgacggc aatccttggt gccctggctg tcttgctcgc cctggcagcc ccggcgtcgg
     3181 cttccggctg gggcgggaac tccggaccag atacctggcc ggtcgctgag ggtggctcgt
     3241 tccggtatca cgtgcagcct ggagccacgg gcgcgcagat gccgagtgcg gtcggattcc
     3301 ggacggagtt tgggcccgta ttcgactcgg ccgaaacgct tgcctcctcc attcttgcaa
     3361 catcgaagat cagccccgtc gatgtctacg tctttgccaa cctgggcctg ttcgaggcga
     3421 cggtcaaggc gcggccggtt gggctgacgc cccactcgac cgtcgtctat gaccccgccg
     3481 ccggcgaggt gctggtcaac ctcacggacg tgctcgccct ctcgcccacg caggttcagg
     3541 atgcgatcgg gaatgccgta acccagcttg cggtccagca tgcgtcatcc ggcaaagcgc
     3601 cggctgggat cgcggcggga attgcgcttt atgtcgaatt accgacatcg gaatacctgg
     3661 cccggatcgc gtcgaccgtc caggcttcct accagaagaa caccttgttg acctggtttg
     3721 atctgaaccg gccgatcgcg gccaaggacc aggagctcgc cctcggcgag tgctacgcga
     3781 tggcctcctt cctgatcgac cggtacgaca tcccggcgct acgcacgatg ctgggcgatc
     3841 tgccgggtgc ggcgcaatgg caggatgcct atcgctccgc cttcgcggcc gaccccagcg
     3901 tgatcgagca gcaatggcgc gccgacctgc ctcgctggac gacatccggg tggcgtgaca
     3961 acctgatcgc gtcgttcgat ctggaaccgg cccgaaccat gctcgggcag ggacaatacg
     4021 tggccgccaa ggcgctgctc gatccttcgc tcaaccttta ccggcagctc aacgatcccg
     4081 aatcgctggc ccagacccag acgctgatga accaggccga caccggcatc caggccgagg
     4141 ccctgatggt cgagatcgaa aaggccctgc aggaccacga ctacgccagg gcatcgaact
     4201 tgctcgacca ggccgacatc cagtacaagg ccctgccgac cgagcaggtg ccgcaatcgc
     4261 tgctctccac ctaccggcaa atggctactg acggacagac tgcgaccgcg cagctcgccg
     4321 aggctgatcg cctggcacgg agttggggca gttatcccga agcccgcgcg gctgcccgcg
     4381 acgccggcgc cacatttgcc cggctcgggg atgagcagaa ccggcagtcg gctgaacagg
     4441 tgctcaacaa gctcgacaac cgtcagcgtc gccttgtcgt gatgatgatc ggtctcggtg
     4501 tcatcacgct ggtttggctc atgctctggc tgcgcgcccg gggtccgagc gatgtgaggt
     4561 gggcctagcc atggcgactc gtgtccttac caccggccga acgatcgcga cgacctggga
     4621 gcgactggcg caaaccctgg cgacgtggtc gcccatggcg atgctcctgc agctccggaa
     4681 tttcctgtgg ccgacctccg catcgctccg ggagcgctgg atgcgattgg tttggctgcc
     4741 gttctggatc ggcaccgaga tcgcggctcc ccacaccggt cgacaggccc gggtgcgcgg
     4801 tgacctgggg ccggccagtc gccggctggt gatgtatctg accagtatcc ggcggcgcca
     4861 actcgtgatc cggtacgcca ccgtgatcgc ccggggcatc gcgctcggac tcctggtctg
     4921 cgcgatctgg gcggcctggg cggcatccgg cgggcgccag atcaacagcg aggcactgct
     4981 gatcacgact ggcatcctgg tcgccatcgg ccttgtcttt ggctggcggc atcgcccgga
     5041 cctgcgggaa acggccacga tgctcgacaa ctccttcgcg ctttccgacc gcacggccac
     5101 cgccttcgat catttgacga ttggcccgaa tcgaatacag ggtcagccgc acctcgccta
     5161 cctgcagatt gccgaggcga ccaatacggt cgtgatgctc aacaagcatt cggcgctccg
     5221 ggtccggccg cccgtgcggg agttggttct gattgcggga ctggccctgc tcttcctcgg
     5281 gctctatctc gtgcgcggca ccggcgcggg gctgccggag gccggcaact cgtcggtccc
     5341 ggctttcgtg gcggccaagg atcggctcca gcagcaaccg acaatcccga ccccgccggc
     5401 cgaggtcaat aaggcaccga gtgtcgagga tgtgcaaaag cgcgccgatc agtcgtccgc
     5461 gactgagcag gatttgctca agatcgccga cgcgctcgcc gacaatcccc tgacggcgcc
     5521 gattgccgat tcgatcaagg ctggcgatta ccagaaggca gccgatcaac tcaatgccgt
     5581 ctcgtctcag gttagccagc tctcgccgga gtcgcgcgcc gatctggccg acaagctgga
     5641 ccaggcggcc gatggcatga cgggccagca cccggatctt caggacgcga cgcacaaggc
     5701 ggcagacggg ctccggtccg atccccagca ggcgcagtcg tcgctctcga acctcagtga
     5761 tcagatcagc aagaccggcc agacggtcga gtcccagcag gctctcgcca aggacatgcg
     5821 cgacgcccgc gaagcgcaag ccagtggatc gtccaaccag ggctcgcgac ccgagccggg
     5881 cgataccaag gctgattcga gccagggcaa tcaacagatt gccggccagg gtggtgatgc
     5941 ctcctccggc cagggacagc agccggatca ggggcagcag accaacggct ccggccagag
     6001 cgattccaac aaaggaaaca ctcagagcca gtcggatcag ggcgggtcga gctcggactc
     6061 gagcagcgca ccgccatccg gccagcagaa cggtgcatcc ggccaatcga ccggtaatgg
     6121 cccggccggt caggttgatc cgtccagcgg caacggatcc ggtcaaacgg gcacgggcga
     6181 gacgtcgggc ccatcccagc cgtcgcaggg caccaccggc ggc
//
LOCUS       DATAOZ010000235         7375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2466093, whole genome shotgun sequence.
ACCESSION   DATAOZ010000235 DATAOZ010000000
VERSION     DATAOZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466093"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1127
                     /locus_tag="VHR64_06270"
     CDS             261..1127
                     /locus_tag="VHR64_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302471.1"
                     /translation="MRSVRITQSVVYGVTALAVASGVFLNIAFANGKFSLSNLWGEHY
                     YAENTVWTYLSIAFVAAIGYLTVRQLPADGVTLKLSRGVTGEGESRVDDVPAAKLVFG
                     SRYVGGILLRPISLFVGLSWVAAGEHKIRDAAWMSNGHALVNPDPQVQGFWERVAAIP
                     EPPARPAIAADFGWYRDFINYMINHGWEAWFGKLIALGEFSIGLGLIFGCLLGVAAFF
                     GIVLNLNFMLAGTASTNPILLVLSVPLIIGYKVASFYGLDRWVLNALGTPWSPGQLFR
                     SPEPTPTASPAD"
     gene            complement(1242..2600)
                     /locus_tag="VHR64_06275"
     CDS             complement(1242..2600)
                     /locus_tag="VHR64_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302472.1"
                     /translation="MALYASTGEMEAAAIEVLRRNDLGGWTKAAPRLYPHQWSWDSAF
                     IAIGLAHVDTARAAQELRSLFLAQWTTGLVPHIVFNPDVPPGSYFPDAESWNVANLSA
                     DVPKGKLTSGLIQPPVHAIAAWRIREIAGQRGRVDLETVDAFLREIYPKLFAWHRYLL
                     TARDPEQSGLVTIYHPWESGLDNSPRWDPALRAVAVGELEPYTRFDLQHVADPSQRPT
                     NEEYDRYLWLVELLKQARYDDAAIAASGYPFMLKDVLISGILVAANEALLRIAEVSGV
                     PDDERSLIADWIERGQRGLESAWDAELGLALDYDVLASEPVRVQTIAGFAPLVAGGLA
                     PARRTALLETLDSEAFLGDARLRWQVPPSTSPLDPAFRSRSYWRGPTWPVLTWLFWWA
                     LMRDGEQEHADRLRTASLDQIATIGFAEYVEPFTGEPLGSLDQSWTAAVALDWLADSP
                     QT"
     gene            2712..5762
                     /gene="treY"
                     /locus_tag="VHR64_06280"
     CDS             2712..5762
                     /gene="treY"
                     /locus_tag="VHR64_06280"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX3302473.1"
                     /translation="MSESESIDGAITLDQRILRTVTDAAASRQPPRATYRMQFHAGFT
                     FRDATKLVPYLADLGISHLYASPIFRANPGSTHGYDVVDYNQLNPEVGSREDFDELVA
                     ALHERGLKLILDFVPNHMGVAAGLNTWWQDVLENGPSSPYAGWFDIDWQPLKPELDNK
                     VLLPVLGDQFGVVLENGELELRLADGAFTVWYYNLPLPIAPPTYGMILRAAVPELGEA
                     LPPEELIALEFESLLAAFERLPGQTEQDPELISDRRRDQTVARAHLADLLERSPEIRS
                     AVEGAVRIFNGTPGDTRSFDPLDALLEAQSYRLAFWRVAAEEINYRRFFAINELAAIR
                     QEEPAVFAATHQLLLELIGTGSVDGVRIDHPDGLWDPAGYLRDLQRAAFAARYRAVWT
                     RNQSPDKIDADWPGIEAALADWWDSAWSTAGDRAGLQPIYLVVEKILEHGETLPGDWA
                     AAGTVGYDFMTETTQLFVDSANVALFDHLYQRFTGLETRFADLAYDKKQMIMRRALAS
                     EVAVLARALDRLTEHQRRTRDFTLNSLRDAMREVIACFPIYRTYLTGSVDRAAPGDRA
                     AIESAIREARRRGQYIDPSVFAFIRSALLLEEPPDQTEAEREDRLRFAMKFQQLTGPV
                     MAKGIEDTVFYIYNRLTSLNEVGGEPATFGLTRETIHQQYGARRTDWPDAMLSSSTHD
                     TKRSEDVRARISSLSEMPRDWRAAINRWSRLNRKHRTRVEGAAAPTRNDEYLLYQTLL
                     GAWPIGLEKPDAAFRDRILAYMDKATREAQVNTSWTNPNEAYDAAVADFIRAILDPKR
                     SQEFHDDFSASRSRVCRAGIFTSLAQQLLKLTAPGVPDIYQGTELWDFSLVDPDNRRP
                     VDYVVRSRLLRALGRRKPTRRLADDLTAGAEDGRIKLYLTARTLWCRADKPALFARGS
                     YQPLEVNGDRAGNVFAFARELDGQQIVVIVPRLIDSLLRGGAEAPIGSAVWGNTTIRL
                     PESGDEVRYRDLFAGGEISGQGEIAIADALAHFPVALLVRTGTE"
     gene            5759..>7375
                     /gene="treZ"
                     /locus_tag="VHR64_06285"
     CDS             5759..>7375
                     /gene="treZ"
                     /locus_tag="VHR64_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX3302474.1"
                     /translation="MTTPHRTPPLGATVGPDGTHFGVWAPRAERIDVQIDGGPSAQLI
                     RDADGVHVGLVPGVETGARYKFRIDGGDAFPDPRSRFQPDGVHGPSEVIDPGAFAWTD
                     ADWPGITMDRLVVYELHVGTYTPEGTFLALIDQLPEIKRLGVTAIELMPVADFPGRWN
                     WGYDGVAPFAPSRAYGRPGDLRQLVDAAHRNGLAVLLDVVYNHLGPDGNYLGVYSADY
                     FTDRHQTPWGNGINYDGANSRFVRDFVIDNGIQWVRDFHFDGLRLDATDTIRDDSELH
                     IMAEFQEKVRAGTDRNIVLIAEESRNTVRTIQPVAQGGLGYDAVWADDFHHSLRVHLT
                     GTREHYYADYEGSVAEITTAINEGFVYQGQRSPRSNRPRGTKVTDEPATGFIVCIQNH
                     DQVGNRPFGERVHHDISMDRYAVASSLLLFLPEPPLLFMGQEFAASTPFLFFTDHNEE
                     LGKLVTNGRRQEFSGFRAFSDPILLNSIPDPQDLLTFLSSRLKLNERDRNAGIYALYR
                     DLLELRRTDSVLRSPSRDRTEAKELGAQILS"
BASE COUNT         1285 a         2424 c         2354 g         1312 t
ORIGIN      
        1 cactgatgcg tacctggaca gaccgggcca gtcaagaaat gacacgtagt tgtaatcatc
       61 gccgacctgg gttttacgcg cagtccatgg gcgagcaggc atcatgccag tgattcgtca
      121 acggcgggca gcgcgggctc gatcgcaaca gggttcaggg gagctccgac agggctgagg
      181 gcgaatcaca acagcggacg ccggaggttc gtccgcttgg gtatcgcctg attgcctcgg
      241 agttgaaagg acggcacagc atgcgatccg tacgcattac ccaatcggtg gtgtatgggg
      301 taacagcctt ggcggtagcc tcgggcgtct tcctcaacat cgcattcgcg aacggcaagt
      361 tctcgctcag taatctgtgg ggcgagcact actacgccga aaacacggtc tggacctacc
      421 tcagcattgc cttcgtcgca gcgatcggat acttgaccgt gcggcagctg ccggctgacg
      481 gtgtcacgct caagctctcc aggggtgtga ccggcgaggg cgaatccagg gttgatgatg
      541 tgcccgctgc caagctggtc ttcggcagcc ggtacgtagg cggcatcctg ctgcgcccga
      601 tcagcctctt cgttggcctc tcatgggtgg cggccggcga gcacaagatc cgcgacgccg
      661 cgtggatgag caatggccac gcactcgtga atcccgatcc ccaggtccag ggcttctggg
      721 agcgcgtcgc cgcgattccg gagccgccgg cccggccggc gattgccgcg gacttcggct
      781 ggtatcgcga cttcatcaac tacatgatca accacggctg ggaggcctgg ttcggcaagc
      841 taattgccct cggtgaattc tcgatcggtc tcggcctgat cttcggttgc ttgctcggcg
      901 tcgcggcctt cttcgggatc gtgctcaacc tcaacttcat gttggccggt actgccagca
      961 cgaacccgat tctcctggtc ttgtccgtgc cgctgatcat cggctacaag gtcgccagct
     1021 tctacggtct cgatcggtgg gtcctgaacg cgctcggtac accgtggagc ccgggtcaac
     1081 tcttccgaag cccggagccc acgccgaccg cctctcctgc tgactagcac cgtcccgggg
     1141 ccggatgatt cacacccgtc cggtccctgg ccgctagtaa accccggcgc gaatgttcgc
     1201 gccggggttt gatcgtgttc agcggtgtgc cgctcccgaa gctacgtttg gggcgaatcg
     1261 gccagccaat cgagggcaac ggcggcggtc caggattggt cgagggagcc gaggggctcg
     1321 cccgtgaagg gctcgacata ctcggcgaag ccaatcgtcg caatctgatc gagcgaggca
     1381 gtgcggagtc gatcggcgtg ctcctgctcg ccgtcacgca tcaatgccca ccagaacagc
     1441 catgtcagga ctggccaggt cgggccgcgc caatagctgc gggatcggaa ggccggatcg
     1501 agtgggctcg tgctcggtgg tacctgccaa cgcaaccgcg catctccgag gaatgcctcc
     1561 gaatcgagcg tttccagcaa tgccgtccgg cgtgctggtg cgaggccacc tgcgacaagc
     1621 ggcgcaaaac cggcaatcgt ctgcacgcgc accggttcag atgcgagcac gtcgtagtcg
     1681 agcgccaggc cgagctccgc atcccacgcg ctttcgagtc cgcgttggcc ccgttcgatc
     1741 cagtcggcaa tgaggctgcg ctcgtcgtct ggcacgcccg aaacctccgc aatccggagt
     1801 agcgcctcgt tagcggcgac caggatgccg ctgatcagca cgtccttgag catgaacggg
     1861 taaccggagg ccgcgattgc cgcgtcgtcg tatctcgcct gcttgagcag ctcgaccaac
     1921 cagaggtagc ggtcgtactc ctcgttcgtc gggcgctgcg aggggtcagc gacgtgctgg
     1981 aggtcgaagc gcgtgtacgg ctccaactcg ccgaccgcga ccgccctcaa cgccggatcc
     2041 cagcgcggcg agttgtcgag gccgctctcc cacgggtgat agatggtgac caagccggac
     2101 tgctcggggt cccgcgcggt cagcaggtag cggtgccagg cgaagagctt cggatagatc
     2161 tcgcgcagga aggcatcgac cgtctccagg tcgaccctac cccgctgccc cgcgatctcc
     2221 cgtatgcgcc aggcggcgat cgcatggacc ggcggctgga tcaacccgct ggtcagcttg
     2281 cccttcggca catcggccga gaggttggcc acgttccagg actcggcatc agggaagtag
     2341 ctgccgggtg ggacgtccgg gttgaagacg atgtgcggga ccagaccggt cgtccattgc
     2401 gccaagaaga ggctccgcag ctcctgcgcg gcccgggccg tatcgacatg ggcgaggccg
     2461 atggcaatga aggcgctgtc ccagctccat tgatgcgggt agaggcgtgg cgcggccttg
     2521 gtccagccgc cgagatcgtt ccggcggagc acctcgatag ccgcagcctc catctcacct
     2581 gtgcttgcat acaacgccat ggcccgcgct ccttgctctc tgtggcgaag attcggactc
     2641 atctcgccct gatgcctgat agtacctgtc gcacgctctg cccggctcga acgacgcccg
     2701 gaggacacca tttgtccgag tccgaatcga tagatggcgc gatcacgctt gatcaacgga
     2761 ttctgcggac ggtgaccgac gccgcggcgt cccgccaacc gccgcgcgcg acctatcgga
     2821 tgcagttcca tgccgggttc actttccgcg acgcgacgaa gctcgtgcca tatctggctg
     2881 atcttggtat cagccacctc tacgcctcgc cgatcttccg ggccaacccg ggcagcaccc
     2941 acggctacga tgtcgtcgat tacaaccagc tcaaccccga ggtcggcagc cgcgaggatt
     3001 tcgacgagct ggttgcggca ctgcacgagc gcgggctgaa gctgatcctc gatttcgtgc
     3061 cgaaccatat gggagttgcg gcagggctca acacctggtg gcaggacgtc ctcgaaaatg
     3121 gcccatcatc gccctacgcc ggctggttcg acatcgattg gcagccgctc aagcctgagc
     3181 tcgacaacaa ggtgctcttg ccggtactcg gcgaccagtt cggcgtggtg ctcgagaacg
     3241 gcgagctcga gctgcggctg gcggacggcg ccttcacggt ctggtactac aacctgccgc
     3301 tgccgatcgc tccgccgacg tacgggatga tcctgcgcgc ggccgtgccg gaactgggcg
     3361 aggcgttgcc gcccgaggag ctgatcgcgc tcgaattcga gagcctgctc gctgccttcg
     3421 agcggttgcc gggccagacc gagcaggatc ccgagctgat cagcgatcgc cggcgtgacc
     3481 agacggtggc cagggcgcac ctggccgatc tgctcgaacg ctcgccggag attcgctcgg
     3541 cggtcgaggg cgcggtccgg atcttcaacg gcacgccggg cgatacacgg tcgttcgatc
     3601 cgctcgacgc gttgctggag gcgcagtcat atcgattggc cttctggcgg gtcgcggccg
     3661 aggagatcaa ctaccgtcgc ttcttcgcga tcaacgagct ggccgcgatc cggcaggaag
     3721 agccggctgt cttcgccgcc acccatcagc tcttgctcga gctgatcgga accgggtccg
     3781 tcgacggagt ccggatcgac cacccggacg gcctctggga ccccgcggga tacctgcgcg
     3841 acctccagcg cgccgccttt gccgcccgct accgcgccgt gtggactagg aatcaatcgc
     3901 ccgacaagat cgatgcggac tggcccggaa tcgaggccgc gctcgccgac tggtgggatt
     3961 cggcatggtc gaccgcaggg gatcgcgccg ggctccagcc gatctatctg gtcgtcgaga
     4021 agatcctcga acatggcgag acattgcctg gcgactgggc ggctgccgga acggtcgggt
     4081 acgacttcat gaccgagacg acgcaactct tcgtcgattc cgcgaacgtc gctctgttcg
     4141 atcacctcta ccagcgcttc accgggttgg aaactcgctt cgcagatcta gcctacgaca
     4201 agaagcagat gatcatgcgc cgggcgctgg ccagcgaggt cgcggtcctc gccagggcgc
     4261 tcgaccgcct gaccgagcac cagcgacgca cccgcgactt cacgctcaat agcctgcgcg
     4321 atgccatgcg cgaagtgatc gcctgcttcc cgatctaccg gacttacctg accggcagcg
     4381 tggatcgggc cgcaccgggc gaccgcgccg cgatcgagtc cgcgattcgc gaggcaaggc
     4441 gacggggcca gtacatcgat ccgtccgtgt tcgccttcat ccggagcgcg ctgctgctgg
     4501 aagagccgcc agaccagacc gaggccgagc gcgaggatcg gctccggttc gccatgaagt
     4561 tccagcaact gacgggtccg gtcatggcca aggggatcga ggacaccgtc ttctacatct
     4621 acaaccggct gacgtcgctc aacgaggtcg gcggcgagcc ggccacgttc ggtctgaccc
     4681 gggagacgat ccaccagcaa tacggcgctc gccgtaccga ttggcccgat gcgatgctct
     4741 cctcctcgac acacgacacg aagcgcagtg aggacgtccg cgcccggatc agctccctct
     4801 ccgagatgcc acgtgattgg cgggccgcga tcaaccgctg gtcccgcctt aaccgcaagc
     4861 accggacgcg ggtcgagggt gcggccgcac cgacccgtaa cgacgagtac ctgctctacc
     4921 agacgctgct cggcgcctgg ccgatcggac tcgaaaaacc tgatgccgcg ttccgtgatc
     4981 gcatcctcgc ctacatggac aaggcgacca gggaggcgca ggtcaatacg agctggacca
     5041 atccgaacga ggcgtatgac gcggccgtgg cggacttcat ccgggcaatc ctcgatccga
     5101 aacgatcgca ggagttccat gacgactttt cggcgtcgag gtcccgcgtg tgccgcgcgg
     5161 ggatctttac ctcgctcgca cagcagctac tgaagctgac cgcacccgga gtgccggata
     5221 tctaccaggg caccgagctc tgggacttca gcctcgtcga tcccgacaac cgccgcccgg
     5281 tcgattacgt ggtccgctct cgtcttctcc gggcgctggg taggcgcaag ccgacgcgga
     5341 gactggcgga tgatctgacc gccggggccg aggatggtcg gatcaagctc tacctgacgg
     5401 cccggacgct ttggtgccgg gcagataaac ccgctctctt cgcgcgcggc tcgtaccagc
     5461 cgctggaggt taacggtgat agagcaggca acgtcttcgc gttcgcccgg gagctggacg
     5521 gccaacagat cgttgtgatc gtgccgcggc tgatcgattc cttgcttcgc ggcggggccg
     5581 aggcgcccat cggtagcgcg gtgtggggga acacgaccat tcgtctgcca gagtcggggg
     5641 atgaggtccg ctatcgggac ctcttcgccg gtggagagat ttcagggcag ggagaaattg
     5701 caatcgccga cgcgctggcg catttcccgg tcgcgctcct ggttcggact ggaactgaat
     5761 gacgacgcca caccgtacac caccgcttgg agcgactgtc ggtccggatg gcacccactt
     5821 cggcgtctgg gcgccgaggg ccgagcggat cgatgttcag atcgacggcg gaccgtccgc
     5881 gcaattgatt cgcgatgccg acggggtcca cgtcgggctc gtgcccggag ttgagaccgg
     5941 tgcccggtac aagttccgga tcgatggtgg ggatgccttc cccgatccgc gcagtcgctt
     6001 ccagcccgac ggcgtccatg gcccgtccga ggtgatcgat cccggcgcct ttgcgtggac
     6061 cgacgccgac tggcccggca tcacgatgga ccgactcgtc gtctacgagc tccacgtcgg
     6121 aacctacaca cccgagggga cctttctcgc cctgatcgat cagctcccgg agatcaaaag
     6181 gctcggcgtg accgcgatcg agctgatgcc ggtcgcggac ttccctggcc gctggaactg
     6241 gggctacgac ggggtcgcgc ccttcgcccc gtcccgcgcc tatggccggc ccggtgatct
     6301 tcgtcaactc gtcgatgccg cgcatcgaaa cgggctcgcg gtcctgctcg acgtcgtcta
     6361 taaccacctc gggccggacg gcaattacct cggcgtctac agtgccgact acttcaccga
     6421 tcgccaccag actccatggg gcaacggcat caactacgac ggcgcgaata gtcgcttcgt
     6481 gcgcgatttc gtgatcgaca acggaatcca gtgggttcgc gacttccatt tcgacggcct
     6541 gcgcctcgat gcgacggata cgatccggga tgacagcgaa ctgcacatca tggccgagtt
     6601 ccaggagaag gtccgcgctg gtacggatcg caatatcgtc ctgatcgccg aggaatcgcg
     6661 caacacggtc cgcacgatcc agcccgtcgc gcaaggtggt ctcggctatg acgcggtctg
     6721 ggcggacgac ttccaccatt cgctccgggt tcacctgacc ggcacgcggg agcactatta
     6781 cgccgattac gaggggtcgg tggccgagat cacgaccgcg atcaacgagg ggttcgtcta
     6841 tcagggccag cgctcgcctc gatcgaaccg gccgcgtgga acgaaggtga ccgacgagcc
     6901 ggcgacggga ttcatcgtct gcatccagaa tcacgaccag gtcggcaacc gacccttcgg
     6961 cgagcgggtc catcacgaca tctcgatgga tcggtacgcc gtcgcctcgt cgctgctcct
     7021 cttcctgccc gaaccgccat tgctgttcat ggggcaggaa ttcgcggcca gcactccatt
     7081 tctcttcttc acggaccaca acgaagaact cgggaagctg gtcacgaatg gacgacgaca
     7141 ggagttcagt ggcttccgag ccttctcgga cccgatcttg cttaactcga ttccggatcc
     7201 gcaggatctg ctgaccttcc tgtcgtcgcg gctgaagctg aacgagcgcg accggaacgc
     7261 aggcatctac gccctctatc gtgacctgct ggagctgcgc aggaccgatt ccgtgttgcg
     7321 gtcgccgagc cgcgatcgga ccgaggcgaa ggagctcggc gcgcagatcc tctcc
//
LOCUS       DATAOZ010000236         7954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8257355, whole genome shotgun sequence.
ACCESSION   DATAOZ010000236 DATAOZ010000000
VERSION     DATAOZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8257355"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VHR64_06290"
     CDS             complement(<1..893)
                     /locus_tag="VHR64_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302475.1"
                     /translation="MVSPRATTSSWSIIGHASQVERLRRMVTAGQYPLAILVTGTPGV
                     GRKALALQFAAASLCQNGHEGVPCTQCRTCGLVAAGRHPDAEIWSVKRQEEESGTASK
                     SGTLTIETVRKIAASTALRPYEGKRRFIIIDEAETLGDPAQQALLKTLEDLPGYATII
                     LISTTAGAMLDTVQSRCMEIALQLVPTVEIAAGVDAPDVGTVAAMAAGRPGWAMRAIS
                     EPEWRASQAEEVERIEAWMKLPRADRLGEAYQRGDRFVKDRKTAFQELDRVQLIWRDI
                     LLASSDLDQFAFDAVRARSLIS"
     gene            complement(893..1657)
                     /locus_tag="VHR64_06295"
     CDS             complement(893..1657)
                     /locus_tag="VHR64_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302476.1"
                     /translation="MRVIRNVSYVRQQKSRAKWLTISGFLLFALGFALVAVLDNVVYS
                     YMTLIPAYVLFIAGMQQLGKWTNSPRRPRGDLLIDETLKHLPERYTMIHYAKVGGTVL
                     EHLLLHPGGALVIVVRDVAGRVELRKKRFRRRANPIGRVIGASGPPLGQPEHELEGGE
                     AILEALLKDRQQEIDVSGVVVFTAVDHELEEIDPELDAIGIADLADYVRVLEPDPSFK
                     QADREQIASLLTAGDGFERNEPVRTRRPVVVKRRAT"
     gene            complement(1743..2489)
                     /locus_tag="VHR64_06300"
     CDS             complement(1743..2489)
                     /locus_tag="VHR64_06300"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEX3302477.1"
                     /translation="MITLIFLALPWSVEHKAHLALHGLCAQRPSHTYTFDGRLLPFDA
                     RMEGIYSGFLVTTVVLFMSGAHRWCRPPSFSRLALIAILGGVMALDGFNSFLKDLALP
                     YLYEPKNWLRLITGMTSGIVLGFALCFLTASSIWKVVDTRRQTLDQLRTVVLVAMLWI
                     PIGLAIMSGWGPLYVPLTLMFIFAASLALTMLSLVVLVILRKRDFTFAGLGSLGGYGL
                     AAVALAVILMGLLSAGRTVLERSIGASPLT"
     gene            2621..3250
                     /locus_tag="VHR64_06305"
     CDS             2621..3250
                     /locus_tag="VHR64_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3302478.1"
                     /translation="MKLWKVVYVAGAVTAAGLIARSVRYGHSAEIDRTITIAIQRRKG
                     KPFARLMWLASWAGFPPQSRTLPFVFPALFALAGLPREAMFQLAGWGTSAISFAVKSV
                     MRRPRPFGDEFTIHKANIGGTSFPSGHVINYIGIYGTLAVILSHRLKSTFLRRLIVGF
                     VGVKIALVGPSRIYLGHHWFTDVLTSYLLGSSYIVLLSSIYKRVARRSS"
     gene            3247..3984
                     /locus_tag="VHR64_06310"
     CDS             3247..3984
                     /locus_tag="VHR64_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX3302479.1"
                     /translation="MSVEVVGHGGAGDFYPGNSRPSIEKALELGVDRIEFDVQRAAGD
                     QIVLVHDDDIWIDGKRHRIHNLAVNEIRRSLDGLLTLDEAIELTRGKCRLLIDIKSPG
                     YETLVADAINSAGIADQTIVSTTHARTLLRIRQCAPGVQLGLSTGHISTVMRKNKLIS
                     VTSGVLAAVTPLPFITTARLIHAENLMLNYRICSPRFVRAAHRSGLRVYAWTVNNPKP
                     IQRLIAQGVDGVISNRPDLVQEALGLR"
     gene            4325..7489
                     /gene="ileS"
                     /locus_tag="VHR64_06315"
     CDS             4325..7489
                     /gene="ileS"
                     /locus_tag="VHR64_06315"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX3302480.1"
                     /translation="MANFKDVSNSVDFPAMERELLDWWLRDGIVEKYLHRNDDSGERY
                     SFLDGPITANNPMGVHHAWGRTYKDLFQRYQNMLGKEQRFQNGFDCQGLWVEVEVEKE
                     LGLKSKRDIETYGVADFVQKCKDRVIKFSARQTEQSIRLGYFMDWDNSYYTMSDENNY
                     TIWHFLKTCHERGWIYKGHDVMPWCPRCGTGISEHEIVTEGYQERSHLSVFVKFPLLD
                     QVNAALLVWTTTPWTLAANVAAAVNPDLTYIEVAQQGWTYYVAEEAAKSALRGDYEVV
                     RTLKGSELVGRPYGGPFDDLPVAKGVRHRVIPWADVTATEGTGIVHIAPGAGKEDFAL
                     SKDNDLPVIAPLNEFGVYVDGFAWLSGQYVHEVAEPIMQDLKKRGVLYRGENYSHRYP
                     VCWRCGTDLVFRLVDEWFISMDELRPQMMEITREVNWVPGFGMDRELDWLAHMDDWMI
                     SKKRYWGLALPIYECGACGTVEVIGSEHELKERAVEGWSDFEGHSPHRPYIDKVKIAC
                     KSCGEVVSRIPDVGNPWLDAGIVAFSTLHYRSDRAYWEKWYPADLISESFPGQFRNWF
                     YSVLAMSTALTGEISFRNVFSYALMRDEHGEEMHKSKGNAIWFEDAAEHMGVDTMRWL
                     FSTVNPSQNLNFGFSVADEVRRRFILPLWNSYAFFANYARLDKFDPTDPATRVHPSER
                     PLLDRWILSRLNQLVQTVRRELDRYEPDAASNEIEQFVVEELSNWYIRRNRRRFWKSE
                     SDADKAAAYHTLYECLTTVVGLLAPFIPFVTEAMYQNLVRSVDSGAPESVHLTDFPVA
                     DESLIDAELSDQMRAVIEAVRLGRAARSTANVKVRQPLPAVLVYTRNREDFDALELLR
                     DQVLEELNVKEIRPLSDVGEVVAYSIRPNLPVLGPKYGKQLGAIRQALAAADPGTVAG
                     LVGADRSVELTLPNGEALWLEPTEVLVDLNKRDGYAAAQGPNMTVALDTELTPELIRE
                     GWIRDFIRGVQDARKTAGLQIEDRIDLRYSASSDIAEAIAANIDYIKSETLSLSIEPS
                     TGGTGSAVEVGDETVTVQVSRAAR"
     gene            7611..>7954
                     /locus_tag="VHR64_06320"
     CDS             7611..>7954
                     /locus_tag="VHR64_06320"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3302481.1"
                     /translation="MRDDRFAAALEQIRAFAEQQLRENGAPGYQIALTDRDGLIAHLE
                     LGCANIDSRAPILPETLFEFGSIGKSFTAICYLQLAEEGRVDLQAPITEYLPWFSVQS
                     VYKPITIHHLLI"
BASE COUNT         1599 a         2505 c         2434 g         1416 t
ORIGIN      
        1 gagatcaacg atcgcgcccg aacggcatcg aacgcaaatt ggtcgaggtc agaactggca
       61 aggagaatat cgcgccagat gagctgcacg cgatccagct cctggaacgc ggtcttgcga
      121 tccttcacga atcgatcgcc acgctggtat gcctcgccga gccgatcagc cctgggcaac
      181 ttcatccagg cctcgatccg ctcgacctcc tcagcctgcg atgcacgcca ctccggctcc
      241 gaaatcgccc gcatcgccca accgggacgg ccggcagcca tggcggcgac cgttccaacg
      301 tcgggagcat cgacgccggc agcgatctcg accgtcggta cgagttggag cgcgatctcc
      361 atgcagcgcg actggaccgt atcgagcatc gcgccggccg tcgttgagat caggatgatc
      421 gtcgcatacc ctggcaaatc ctcgagcgtc ttgagcaagg cttgctgggc cggatcgccg
      481 agcgtctccg cttcgtcgat gatgatgaag cgtcgcttgc cctcgtacgg tcgaagggca
      541 gtggatgcgg cgatcttgcg gacggtctcg atcgtcaacg taccggactt cgaggcagtg
      601 ccggactcct cttcctgccg cttgaccgac cagatctccg catcgggatg ccgtccggcc
      661 gcgaccagcc cgcacgtgcg gcactgagta cacggaaccc cttcatggcc attctggcaa
      721 agcgaggctg ccgcaaattg cagagcgagc gcctttcggc caacgcccgg cgtcccggtt
      781 accaggattg ccagcggata ctgacccgcg gtgaccatgc ggcgaagccg ctcaacctgg
      841 gatgcatggc cgatgatcga ccaactgctg gttgtggcac gggggctgac catcaggtcg
      901 cgcggcgctt gacgacgacc ggccgtctgg tgcggaccgg ttcattgcgt tcgaatccat
      961 caccggccgt caacaacgat gcaatctgct cgcgatccgc ctgcttaaag gatggatccg
     1021 gctcgagcac ccggacgtaa tcggccagat cggcgatccc aattgcatcc agctcgggat
     1081 cgatctcctc gagctcatga tcgactgccg tgaagacgac gacaccggag acatcgatct
     1141 cctgctggcg atccttcagc aacgcctcga gaatcgcctc gccgccttcg agctcatgct
     1201 cgggctgacc gagcggcgga ccactggcgc cgatgacgcg accgatcggg ttcgcccggc
     1261 ggcggaatcg cttcttgcgc agctcgaccc tgccggcaac gtcacgcacc acgatcacaa
     1321 gcgcgccacc ggggtggagc agcaggtgct ccagcaccgt gcctccgacc ttggcgtaat
     1381 ggatcatcgt gtagcgctca ggcaggtgct tgagcgtctc atcaatcagg aggtcaccgc
     1441 gcggtcggcg cggcgagtta gtccatttgc cgagttgctg cataccggca atgaagagga
     1501 catacgccgg gatcaacgtc atgtaggaat agacgacgtt gtcgaggact gccaccagcg
     1561 caaagccgag ggcaaagaga agaaatccac taatcgtgag ccacttggcg cgacttttct
     1621 gctgtcgcac atagctcaca ttacgaatga cacgcatgaa caccgccccg ggggcaatga
     1681 gaggaatctc agacgggttc gcattgaacc accgctgagt ttagcacaag gttgacttca
     1741 gattatgtca ggggagatgc cccaatcgac cgctcaagca ccgttcgccc ggccgagagc
     1801 aggcccatca gaatcacagc cagcgcaacc gcggcaagcc catatcctcc caggctgccc
     1861 agaccggcaa acgtgaaatc gcgtttcctg agaataacca agaccaccag agagagcatt
     1921 gtcaaggcga gcgaagccgc gaagatgaac atcagtgtca gcggcacata gagcggaccc
     1981 cagcccgaca tgattgccaa tccgatgggt atccacagca tcgcgaccag gacgaccgta
     2041 cggagttgat caagtgtctg gcggcgcgtg tcgaccacct tccaaatcga cgacgccgtc
     2101 aggaaacaga gcgcgaaacc aaggacgatc cccgatgtca tgcccgtgat caggcggagc
     2161 cagttcttcg gctcatacag gtacgggagc gccaagtcct tcagaaacga gttgaaacca
     2221 tcgagcgcca tcacgccgcc gaggattgcg atcagggcga gccgcgagaa cgacggaggc
     2281 cggcaccacc ggtgcgcgcc gctcatgaac agcacgactg tcgtgaccag gaacccggaa
     2341 tagatccctt ccatgcgggc atcgaatggc aacagccggc cgtcgaacgt gtaggtgtga
     2401 gacgggcgct gcgcgcaaag gccatgcagg gcgaggtgag ccttgtgttc gacgctccag
     2461 ggaagcgcaa ggaaaatcag ggtgatcaac aatgggatca aaatgagcgg ttgtatcgcc
     2521 cgcggtgatc gcaatggaat acgattggaa aacgtggtcg ccatgcgttt cattctcgca
     2581 cggcgtgcaa tcgaactctg gactggatgg cgctcgaacg atgaagctgt ggaaggtcgt
     2641 gtacgtcgcg ggcgcggtca cagcggccgg cttgatcgcc cgctcggtcc ggtatggaca
     2701 ctcggcggag atcgaccgaa ctatcaccat cgcgatccag cggcgcaaag gcaagccgtt
     2761 cgcgcgcctg atgtggctgg cgtcgtgggc agggtttcca ccccagagcc ggacgcttcc
     2821 gttcgtcttc cccgccttgt tcgccctcgc cggtttgcct cgagaagcta tgttccagct
     2881 tgccgggtgg ggcacgagcg cgatctcgtt cgcggtcaag agcgtgatgc gccggccgcg
     2941 ccccttcggc gatgagttca cgatccacaa ggccaacatc ggtggcacga gcttcccgag
     3001 cggtcacgtc atcaactaca tcggcatcta cggcaccctc gcggtcatcc tgtctcaccg
     3061 gctgaaatcg acgtttctgc gccggctcat cgtcgggttc gttggagtca agatcgccct
     3121 ggtcggaccc agccggattt acctcgggca tcactggttc accgacgtcc tgacctcgta
     3181 cctgctcgga tcgagctaca tcgtgctcct cagctcgatc tacaagcgcg tcgcgcgccg
     3241 gtcgtcatga gcgtcgaggt cgtgggacat ggcggcgccg gcgacttcta ccccggcaac
     3301 agccgcccat cgattgagaa agcgcttgag cttggcgtcg atcgtatcga atttgatgtt
     3361 cagcgagcgg ccggcgacca gatcgtgttg gtccacgacg acgacatctg gatcgatggc
     3421 aaacgacacc gaatccataa cctggcggtg aacgagatcc gtcgatcgct ggacggattg
     3481 ctcacgctgg acgaagcgat tgagctcact cggggcaagt gccggctgct gattgatatc
     3541 aagtctcccg gttacgaaac gcttgtcgcc gacgctatca actcggccgg gatagcggat
     3601 caaaccatcg tctcgacaac gcatgccagg acccttctcc gcatcagaca atgcgcgccg
     3661 ggcgtgcaac ttgggctatc caccggtcac atttccacgg tcatgcgcaa gaacaagttg
     3721 atctcggtaa cgagcggcgt actggcggcg gtgacgcccc ttccgttcat cacgacagca
     3781 cgactgattc acgccgagaa cctgatgctc aattaccgca tctgttcgcc ccgattcgtt
     3841 cgcgctgccc accgatccgg actccgggtc tatgcctgga ccgtcaacaa cccaaagccg
     3901 attcagcggc tgatcgcgca aggagtcgac ggcgtaatct cgaaccggcc tgatctcgtg
     3961 caagaggcac tcgggctgcg ctgatccggg actcctggca tccttgcgat cgcgcgggcc
     4021 agagtgctag gctcaacatg aatacggcag tgcggaagcc atgccgggtt cttcagcgtc
     4081 tcagcgagac cggggtggtg agagccggtc gaccccctgg ggagccgcgg aatcccttcc
     4141 aagccccggc ccgaactcca tcccggacag taggcgtcgg cgggtgcgcc cgataccgcg
     4201 tttcgagtgg gtcctctccg tgccggatgg gatcaatcga ggtggtacca cggattctcg
     4261 ccgtcctcag ctgagggcgg ctttcttgtt gccatagacg atcgacacgg ctcaggagca
     4321 aagcgtggca aatttcaagg atgtctcgaa ttcggtcgat tttccggcca tggagcgcga
     4381 gctgctcgac tggtggctgc gcgacgggat cgtcgagaag tacctccacc gcaacgatga
     4441 ctccggcgag cgctactcct tcctggacgg tccaatcaca gccaacaacc cgatgggcgt
     4501 gcatcacgcc tggggccgga cctacaagga cctcttccag cgctaccaga acatgctcgg
     4561 caaggagcag cggttccaga acggattcga ttgccagggc ctttgggtcg aagtcgaagt
     4621 cgaaaaggag ctcggcctca agagcaagcg cgacatcgag acctacggtg tagccgactt
     4681 cgtccagaag tgcaaagacc gggtgatcaa gttcagcgct cgccagaccg agcagtcgat
     4741 ccggctcggc tacttcatgg actgggacaa cagctactac acgatgtcag atgagaataa
     4801 ctacaccatc tggcacttcc tgaagacttg ccatgagcgc ggctggatct acaaaggaca
     4861 cgacgtcatg ccctggtgcc cgcgctgcgg aaccggtatc tccgagcacg agatcgtgac
     4921 cgaggggtac caggagcgct cccacctctc agtcttcgtc aagttcccgc tgctcgacca
     4981 ggtcaacgcc gcgctgctgg tctggacaac cacgccatgg acgctcgccg ccaacgtcgc
     5041 ggccgccgtc aacccggatc tgacctatat cgaggtcgcg cagcagggct ggacgtatta
     5101 cgtggctgaa gaggcagcca agtcagctct gcgtggcgat tacgaggtcg tgcggacgct
     5161 taagggcagc gagttggtcg gacgcccgta cggcggacca ttcgacgacc tgccggtggc
     5221 caagggagtc aggcaccggg tcatcccgtg ggcggatgtc acggcgaccg aaggaaccgg
     5281 aatcgtccac atcgcgcccg gcgccggtaa ggaagacttc gccttgtcga aagacaacga
     5341 cctgccggtg atcgcgccgc tcaacgagtt cggcgtgtac gtcgacggat tcgcatggct
     5401 ctccgggcaa tacgtccatg aggtcgccga gccgatcatg caggatctca agaagcgcgg
     5461 cgtcctctat cgcggcgaga actacagcca ccgctacccg gtctgctggc gttgcgggac
     5521 cgaccttgtc ttccggctgg tcgacgagtg gttcatctcg atggatgagc tgcggcccca
     5581 gatgatggag atcactcggg aggtcaactg ggtccccggc ttcggcatgg accgcgagct
     5641 cgattggctc gcgcacatgg acgactggat gatctccaag aagcggtact ggggtcttgc
     5701 cctgccgatc tacgagtgcg gagcctgcgg cacggttgag gtcatcggca gcgagcacga
     5761 gctcaaggaa cgcgcggtcg agggctggtc cgacttcgag ggtcactcgc cgcaccgccc
     5821 atacatcgac aaggtcaaga tcgcctgcaa gagctgcggt gaggtagtca gccggatccc
     5881 ggacgtcggc aatccgtggc tcgacgccgg catcgtcgcc ttctcgacgc tccactatcg
     5941 ctcggatcgt gcctattggg agaagtggta cccggccgac ctgatcagcg agagcttccc
     6001 gggccagttc cgcaactggt tctactcggt gctggccatg agcacggctc ttaccggcga
     6061 gatctcgttc cggaatgtct tttcctatgc cctgatgcgc gacgagcacg gcgaggagat
     6121 gcacaagagc aagggcaatg cgatctggtt cgaggacgca gccgagcaca tgggcgtcga
     6181 cacgatgcgc tggctctttt cgacagtcaa cccgagccag aacctgaact tcggcttcag
     6241 cgtcgccgat gaagttcgga ggcgcttcat cctccccctc tggaactcgt acgccttctt
     6301 tgccaactat gcgcggctcg acaagttcga ccccaccgac ccggcgacgc gagtgcaccc
     6361 gtccgagcgg ccgctgctcg accgctggat cctctcgcgt ctcaaccagc tcgtgcagac
     6421 cgtccgcagg gaactcgatc ggtatgagcc ggatgcggcc tcgaacgaga tcgagcagtt
     6481 cgtggtcgaa gagctttcga actggtacat ccgccggaat cgccgccgtt tctggaagag
     6541 cgagagcgat gccgacaagg ccgctgccta ccacacgctc tacgaatgcc tgaccaccgt
     6601 tgttggactg ctcgctccat tcatcccgtt cgtgaccgag gcgatgtacc agaacctggt
     6661 ccgttcagtg gattccggag cgccggagtc ggtgcacttg accgatttcc cggttgccga
     6721 cgaatcgctg atcgacgcgg agctgtcgga ccagatgcgc gcagtcatcg aggcggtcag
     6781 attgggccga gctgcgcgaa gcacggccaa tgtcaaggtt cggcaaccgc tgccggctgt
     6841 gctggtctac acccgcaacc gggaggactt cgacgctctt gagctcctgc gcgatcaggt
     6901 cctggaagag ctcaatgtca aggagatccg gccgctttcg gacgtcggcg aagtcgtggc
     6961 ctacagcatc cggccaaact tgccggtcct cggcccgaag tacggaaagc agctcggtgc
     7021 gatccggcag gcgctggccg cggccgatcc gggaacggtc gctgggctgg taggagccga
     7081 ccgcagcgtt gagctgacgc tgccaaacgg cgaagctctc tggctcgagc caaccgaggt
     7141 gctggtcgac ctcaacaagc gagatggcta tgccgccgcg caaggcccaa acatgacggt
     7201 cgcgctcgac acggagctga cgccggagct gatccgggag ggttggatcc gcgacttcat
     7261 ccggggcgtg caggacgccc gcaagacggc cggtctgcag atcgaggatc ggatcgacct
     7321 gcgctactcc gcctcatccg acattgcgga ggcgatcgcg gctaatatcg actatatcaa
     7381 gtccgagacc ctgtcgcttt cgatcgaacc atcgacaggc ggcacgggca gcgcggtcga
     7441 agtcggcgac gaaacggtca ccgtccaggt atcgcgcgcc gctcggtaga gcccgccgtc
     7501 accgaactgc ccgctgaatt cgagatgccg gagtcaatgg actccggcat cgtcatgttc
     7561 ggggatactg cgcgacagca atgttggaac gaccaagggg agcgttggca atgcgcgacg
     7621 atcgatttgc cgcggcactg gagcagattc gagcgttcgc cgagcagcaa ttgcgcgaga
     7681 acggcgcgcc cggataccag atcgcgctga ccgaccgcga cggcctgatc gctcacctcg
     7741 agcttggctg cgccaatatc gactcgcgcg cgccgatcct gcccgagacg ctgttcgagt
     7801 tcggctccat cggcaagtcg ttcaccgcga tctgctacct tcaactcgcc gaggaagggc
     7861 gagtcgacct ccaggcgcca atcaccgagt acctgccctg gttctcggtg caatcggtct
     7921 acaaaccgat cacgattcat cacttgctga tcca
//
LOCUS       DATAOZ010000237         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2731986, whole genome shotgun sequence.
ACCESSION   DATAOZ010000237 DATAOZ010000000
VERSION     DATAOZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2731986"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..884)
                     /locus_tag="VHR64_06325"
     CDS             complement(249..884)
                     /locus_tag="VHR64_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3302482.1"
                     /translation="MQFASGQVLWESGATIHSLYFPRTCVISLLTTLSDEQPVEAATV
                     GREGMVGTPVVLGVRVTNAQALAQVPGIAIRLDAERFLSDLRRSNGAFSGLLLRYAQA
                     LHEQTAQSVACNRRHPIEERCARWLLMTQDRVGANQFPLTQEFLAFMLGVRRASVTVA
                     AGILQQAGLISYQRGKISILDREGLEAASCECYAVVRRKYEQLLGGDTETA"
     gene            complement(1384..2244)
                     /locus_tag="VHR64_06330"
     CDS             complement(1384..2244)
                     /locus_tag="VHR64_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3302483.1"
                     /translation="MPTITTPDGIEIYYKDWRTGQPIVFSHGWPLSADDWDKQMMFFL
                     DRGFRVIAHDRRGHGRSTQTADGHDMDHYADDLAALTGHLDLREAVHVGHSTGGGEVV
                     RYLARHGESRVAKAAIISAVPPLMVRTPANPGGLPKEVFDDYQTQVATNRAQFYYDVP
                     AGPFYGYNRPGAKPSQGLIWNWWRQGMMGGAKAHYDGVVAFSQTDFTEDLKKITVPVL
                     VMHGDDDQVVPYADSAPLSAKLLANGTLKTYNGFPHGMPTTEAETINRDLLAFIKQPA
                     ARRAPEMVGV"
BASE COUNT          401 a          768 c          777 g          456 t
ORIGIN      
        1 ccacgccgcc agattgaccg atcggcctct cgtggctcga cgatccgtcc tgaggaaaat
       61 ccataacggc gcgacctgtt tcggttatgt cgattcggtg ctggtccccg ccaccaacta
      121 cgtacaggtt aggatcggag gagttggcgg tcaagtcttt gtgcaccatg atgttacgga
      181 gacttttttg tacgtcaacg gacgttgccc cgtacgctgg cgtacatgaa atggctcctt
      241 tgagccggtc atgcggtctc ggtgtcccct cccagcaatt gctcgtattt ccgccggacg
      301 acggcgtagc actcacaact cgcagcttca agcccttcgc ggtcgagaat cgaaatcttc
      361 ccgcgttggt aactgatgag tcctgcctgt tggaggatgc ctgccgcgac cgtcacgctc
      421 gcccgccgta cgccaagcat gaatgcgagg aactcttggg tgagcgggaa ttggttcgcg
      481 ccgactcggt cctgcgtcat gagcaaccaa cgcgcgcagc gctcctcgat cgggtgacga
      541 cggttgcagg ccaccgactg cgccgtttgc tcgtgcagcg cctgcgcgta ccgtaggagg
      601 agcccggaga atgcgccgtt ggaccggcgc agatccgaca ggaaacgctc cgcgtccaga
      661 cggatcgcga tgccgggcac ctgcgcgagt gcctgggcgt tcgtcacccg aacaccaagc
      721 acgaccggtg ttccgaccat cccctcccgc ccgactgtcg cagcctcgac cggttgctcg
      781 tcggacagcg tagtcagaag cgagatcaca cacgtgcgag ggaagtacag ggagtggata
      841 gtcgcgcctg actcccagag gacctgcccg gacgcaaact gcacgggctc cccgtcgccg
      901 aggagcgcag cgtaccggtc ggaaggcagg gatcggagaa gacgattctc ctcgacgggt
      961 ggcccttgat ggggctgcat ggcccttcca gatctgccgt gtggtgaaac caccatctgt
     1021 cggcagaggg cccaacgctg gccggagcct gtgtcgagag cgtgggctcg gcgagcccat
     1081 cgaaccaagt gaatcggtcc gcagagttgt aggatcgccg gtgtgttcgg tcgaggcaag
     1141 ccggtcgggc cagcgatttt cgctctcgga tccagtcgac ctcgcggctc cttcttggga
     1201 tctgcgaagg tccacgtgtt cccttgacgg accgatgcgc ctaacgcggg cgtccgcgca
     1261 gctggttcgg gcgacgcgat tcacggcaaa taccaccggc atcggtcgag ccggggaggg
     1321 tcgatcctcc ccggccgcat cgtgaccccg gccccaccga tcagggacat cgttaggcca
     1381 ccgtcagacc ccgaccatct ctggcgcccg gcgtgccgcc ggctgtttga tgaacgcgag
     1441 aaggtctcgg ttgatcgtct cggcctccgt cgtgggcatc ccgtgtggga agccattgta
     1501 ggttttcagc gtgccgttcg cgagcagctt cgcggacaac ggggccgaat cggcgtacgg
     1561 cacgacctgg tcgtcatcgc cgtgcatgac cagaaccggc acggtgatct tcttcaggtc
     1621 ttcagtgaag tccgtctgcg agaaggcgac gacaccgtca tagtgcgcct tcgcgcctcc
     1681 catcatgccc tggcgccacc agttccagat caggccctga gagggcttcg cgccgggacg
     1741 gttgtacccg tagaacggtc ccgccggcac gtcgtagtaa aactgggccc ggttggtcgc
     1801 gacctgcgtc tgatagtcgt cgaacacctc cttgggcagc ccgccggggt tcgccggcgt
     1861 ccgtaccatc aacggcggca cagcgctgat gatcgctgct tttgccaccc ggctctcgcc
     1921 gtggcgcgcc agatagcgca cgacttcgcc accgccggtc gagtggccga cgtgcaccgc
     1981 ttccctgagg tcgagatggc cggtgagtgc cgcgagatca tcggcgtagt gatccatgtc
     2041 gtggccatcg gcggtctggg tggaccgccc atggccgcgc cgatcgtggg cgatgacgcg
     2101 gaagccgcgg tcgaggaaga acatcatctg cttgtcccaa tcatcggccg agagcggcca
     2161 gccgtggctg aacacgatcg gctgtccggt gcgccagtcc ttgtaataga tctcgatgcc
     2221 gtcgggggtc gtgatggtcg gcatatgact gcctcctgcg aaagggtttg ggagttggtt
     2281 cgttcccaag atcggccctt ccgcgcaggc agcattggac cgtggtgcct ccccgttcgg
     2341 ggaaacctcc gtaggtggcg ttcgcctcgg gccatcgcaa cgcgggtcgc ccgcgttcgg
     2401 cg
//
LOCUS       DATAOZ010000238         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7204800, whole genome shotgun sequence.
ACCESSION   DATAOZ010000238 DATAOZ010000000
VERSION     DATAOZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7204800"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..924
                     /locus_tag="VHR64_06335"
     CDS             52..924
                     /locus_tag="VHR64_06335"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3302484.1"
                     /translation="MTVRIGLQLYSVRNSLARDPWGTLARLAEAGFTHLEAANHHART
                     DPGVGFGVAASELRAQLANLGLSIIGCHINPLELDNLPRALDYQAELGNTQFGCDIEF
                     FPYGDRDYVLRRAEMFNKVGELARERGMRFYYHNHFQEFQRIGDDYVYDLILENTDPD
                     LVKLELDTYWMYRGGQDPIKWMHKCADRVILVHQKDFPADAPQPLNLYDGVVSPTENI
                     DMAVFEERKDPRCFTEIGTGVLPIQRILDAAGALPAVDYLILEQDHTAMDEIESVRTS
                     RNAFASNFTGISWS"
     gene            1096..2358
                     /locus_tag="VHR64_06340"
     CDS             1096..2358
                     /locus_tag="VHR64_06340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3302485.1"
                     /translation="MTSALIGGRYELLETIGEGGFAVTWRAWDTRLERAVALKILRPQ
                     YARDERFVFRFVREARAAASVSHPNVVRLYDFGQDTDIVFIAMQYVQGQTLRDLLRRF
                     PGGMPESDVIKVIAPVLRGLSAVHEAGIVHRDVKPDNVLIDRDGEVLITDFGIALLAD
                     SPRLTEASTTFGTAAYMAPEQGRGDPVSPATDIYAIGVVLFELLTGRLPFIADNPVAM
                     MMAHQNQSLPFPSQYVPGGVSPSVQSATMRALAKRPEDRFPSAEAFLDALTGRTIAAT
                     IQARPVTATMPMAAAPRLRATPSPQLAAPPVTRRRSSPLPWLLLLVLIAGGAALGAAY
                     AAGYFDTSEDPGQRLVVVTPTPTPPPEDTPTTIDAPTIAPVEDVPTEVIETPTEIIEP
                     PTEDTGPGMIESAVPDSSPQGGIESNGP"
     gene            complement(2408..>3679)
                     /locus_tag="VHR64_06345"
     CDS             complement(2408..>3679)
                     /locus_tag="VHR64_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302486.1"
                     /translation="VVAALWHLWPAAESTPFHRDEARWIGNSALLREWRHPLGSRWQD
                     EGYRNIYGTLDESNRRRSQPPLAMYAFGIGLIIQDEGLQTTGYWIMSHDDEWNAAHGH
                     MPSRGELLAARRTNVVIAMLTALGMFAIGMKLTNRIGGLAMGLAYALHPLVLDTSTRA
                     WSDPLLVLCVVAAGVAAIHFGERPSFGRAAVVGICLGLGGATKLSPLILAVAIGSSGL
                     LPVAWGLHFRWRVAVRSGLALISIPVVAALTFLASYPYLWTDPIGHARRLFAFRSLSF
                     DLQALISPHARVSGLGDATRRFGVQLAERDSVGGFLIDSLNLDSSWSWLTNLDLVLAA
                     LGWLLLVALLFRREFDAGMTMPVLVIGGQAALIAATFRLDYARYMLPLVPAVVLGIGV
                     VAGLAWSASVRYARAVTMERHLTTALTDSTS"
BASE COUNT          691 a         1249 c         1132 g          607 t
ORIGIN      
        1 gtcaaccgcc ggccaaatgc cgccaatcga acaggacgca aaggacccac gatgaccgta
       61 cgcattggac tccagctcta ttctgtgcgg aattcgcttg cccgtgatcc atggggaacg
      121 ctcgcccggc tggctgaggc cggcttcacc cacctcgaag ccgccaacca ccatgcgcgc
      181 accgatccag gggttggctt tggcgttgcg gcatccgagc tccgcgccca gctcgccaac
      241 ctcggcctct ccatcattgg atgccatatc aacccgctcg agctggacaa tctcccgcga
      301 gccctggatt accaggccga gctcggcaac acccagttcg gctgcgacat cgagttcttt
      361 ccgtacggcg atcgcgacta cgtgctgcgg cgcgccgaga tgttcaacaa ggtgggcgag
      421 ctggcccggg agcgcgggat gcgcttctac tatcacaacc atttccagga gtttcagcgc
      481 atcggtgacg actacgtcta cgacctcatt ctcgagaaca ccgaccccga tctcgtcaag
      541 ctggagctcg acacgtattg gatgtatcgc ggcggtcagg acccgatcaa gtggatgcat
      601 aaatgcgccg accgcgtgat cctcgtgcac cagaaggact tccccgctga cgcgccgcag
      661 ccgttgaacc tgtacgacgg agtcgtctcg ccgaccgaga acatcgatat ggcggtcttc
      721 gaggagcgca aggacccgcg ctgctttacg gagatcggca cgggcgtgct cccgatccag
      781 cgaattctcg atgccgccgg agcgctcccg gccgtcgact acctcatcct cgaacaggat
      841 cacaccgcca tggacgaaat cgagtccgta cgcaccagcc ggaacgcgtt cgcctcgaac
      901 ttcaccggta tctcctggag ctagtggtct ggcaccgttg atgtggagac ggcgccacgc
      961 ccgagccggc catttcgagc atgcgatctg agcgcgcgcc tgtctctggc gtaccattga
     1021 tgtcgccggg ggtgcagtgc ctccggatct cttccgaggc ggtggcttgt tgacgcggac
     1081 cgtccaactg gtggcatgac aagcgcgttg atcggcggcc gctacgaact cctggaaacc
     1141 atcggcgagg gcggcttcgc ggttacctgg cgtgcctggg atacgcgcct ggaacgtgcc
     1201 gtcgcgctga agatcctcag gccgcagtat gcgcgggatg agcgattcgt gttccgcttc
     1261 gttcgcgaag cgcgcgcagc cgcttcggtc tcgcatccga acgtggttcg cctttacgac
     1321 ttcggccagg acacggacat cgtcttcatc gcgatgcagt acgtccaggg tcaaaccctg
     1381 cgcgatctgc tgcggcgctt tcccggcggc atgcccgaat ccgacgtcat caaggtgatc
     1441 gcgcccgtcc tgcgcggcct ctcggcggtc cacgaagccg ggatcgtgca ccgcgacgtc
     1501 aaaccggaca acgtcctgat cgaccgggac ggcgaggtcc tgatcaccga cttcggcatc
     1561 gccctgctgg cagatagccc gcgcctgacc gaagcgagca cgacgttcgg caccgctgca
     1621 tatatggcgc ccgagcaagg tcgcggggat ccggtctcgc ccgcgaccga tatctatgcg
     1681 atcggagtcg tgctgttcga gttgctgacc ggccggctac cgttcatcgc cgacaacccg
     1741 gtcgcgatga tgatggcgca ccagaatcag tcgctgccct tcccgagcca gtacgtcccc
     1801 ggcggggtgt cgccgtctgt ccagtccgcg acgatgcgcg cgctcgccaa acggccggag
     1861 gatcgtttcc catcggcgga ggcattcctg gacgcgctga ccggccggac catcgcggcg
     1921 acgatccagg ctcgcccggt cacggccacg atgccgatgg cggccgcacc tcgtcttcgg
     1981 gccacgccat ctccgcaact tgccgctccg ccggtcacgc gccgacgttc ttcaccgctg
     2041 ccctggctgc tgctgttggt gctgattgcc ggtggcgcgg cactcggcgc ggcctatgcg
     2101 gccggctatt tcgatacgtc cgaggatcca ggacagcgtc tggtcgtggt gaccccaacg
     2161 cccactccgc cgcctgaaga cacgcccacc acgatcgacg caccgacgat tgcacccgtc
     2221 gaggacgtgc ccacagaagt aatcgagaca ccgaccgaga tcatcgagcc gccaaccgag
     2281 gatactggtc ccgggatgat tgaatccgcg gttccggaca gctctcccca gggcgggatt
     2341 gagtcgaacg gcccgtaata cagcgactcg actccattgg attaggagtt ggcagacccg
     2401 ttcaacgtca cgaggtggaa tccgtcaatg cggtggtgag gtgacgctcc attgtgacgg
     2461 cccgtgcata cctgactgag gccgaccacg ccaggcccgc aaccaccccg atgcccaaca
     2521 cgacggccgg caccagcggc agcatgtacc gtgcgtaatc gagccgaaat gtcgcggcga
     2581 tcagggccgc ctgaccaccg atgacgagca ccggcatggt cataccggcg tcaaactcgc
     2641 gccggaacaa cagcgctacc agcagaagcc agccgagcgc ggccaatacg aggtcgaggt
     2701 tcgtcaacca cgaccagctt gaatccaggt tcagggaatc gatcaggaaa ccgccgacgc
     2761 tgtcccgctc cgcaagctgc acgccgaacc ggcgcgtggc gtcgccgaga ccggatacgc
     2821 gcgcgtgagg cgagatgaga gcttgcagat cgaagctcaa cgaccggaac gcgaacaagc
     2881 gccgcgcgtg cccgattggg tcggtccata ggtatgggta cgaagccaga aacgtcaatg
     2941 ccgccacgac cgggatcgag atcagtgcta atcccgagcg gaccgccacc cgccaccgga
     3001 agtgcaagcc ccatgctacc ggcagcaacc cggagctgcc gatcgcgaca gccagaatta
     3061 acggactcag cttcgtcgcg ccgccgagac cgaggcagat cccgacaacg gcggctcggc
     3121 cgaacgacgg tcgctcaccg aagtggatcg ccgcgacccc ggcagcgacc acgcagagca
     3181 ccagcagcgg gtccgaccag gcccgggtcg aggtatcgag gacaagcgga tgcagggcat
     3241 aggcgaggcc catcgcaagc ccgccgatcc ggttcgtcag cttcatcccg atggcgaaca
     3301 ttcccagcgc ggtgagcatg gcgatgacga cattggttcg gcgggcggcc agcagctcgc
     3361 ctcgcgacgg catgtggcca tgcgcagcgt tccactcgtc gtcgtggctc atgatccagt
     3421 agccggtcgt ttgcagaccc tcgtcctgga tgatcaaccc gattccgaag gcatacattg
     3481 ccagcggcgg ctgcgaccga cggcgattgc tctcgtcgag cgtgccgtag atgttgcgat
     3541 atccctcgtc ctgccaccgg ctgccgagcg ggtgccgcca ctcccgcagc agggcggagt
     3601 tgccgatcca gcgcgcttcg tcccgatgaa atggagtcga ttcagccgcc ggccaaaggt
     3661 gccacaaggc cgcgacgac
//
LOCUS       DATAOZ010000239         4206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3257085, whole genome shotgun sequence.
ACCESSION   DATAOZ010000239 DATAOZ010000000
VERSION     DATAOZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3257085"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1405
                     /gene="mgtE"
                     /locus_tag="VHR64_06350"
     CDS             56..1405
                     /gene="mgtE"
                     /locus_tag="VHR64_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX3302487.1"
                     /translation="MRNVEQLQIELREHLHSLERSGVVGLSATFQPAEWAAIVPRLEH
                     DELVTLFDWLPDDDAATVLAALDPHTAADVFRAIGPVQAAAALDEMPEDDAADVLEAL
                     PDEHANALLTAIPADEAEELRDLGQFDPDTAGGIMSPGFVAMAPSLHADQAISALRKI
                     AAERQPVTYTYILDDVGRLLGVLSLHALVLAAPDTLVTDLMVQDVVRVRADTEATVAA
                     QLLRTHRLLALPVVSDDDQLLGLITPDDVANLALEEAIEDIERLGGSQPLETSYRAAS
                     ITLLVRRRLGWLMVLFLAEMYTSTVLKHYDGDIGRLPLLSIFIPLLTGVGGNVGSQTV
                     GTLIRSMAIGEVRLRDIRWVVAKEFGTAALMGLGMAVVAFGRAEWLGVGHDLALVVAL
                     SIIVICAWSATVAAVLPLILRALRIDPALVSAPLITTLVDGTGLMIYFTIAEFIVLR"
     gene            complement(1418..2509)
                     /locus_tag="VHR64_06355"
     CDS             complement(1418..2509)
                     /locus_tag="VHR64_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3302488.1"
                     /translation="MVRLAVLGCGDVAFRSYFSGLKTLGDSVEVVALCDPIADRAIRA
                     ASLFPGADALSTLTALTERTDIDGVINLTPAPFHRETTTALLSAGFHVLSEKPLAATV
                     ADGRELIAHAESVGKLLLCAPAVMATPRVQWIQEVIQSGAIGRVTHAFARLATMGPAG
                     WNEYTGDPAVFYMAGVGPAVDLGVYLITTLTGLFGPAQRVQAMGGILIPERTITIPRL
                     AGQKVTVQAPDLISMQVEFADNRYATIISSFSMPSHRGPVLEVIGERGIASVVGEMEY
                     WVGQGVDLFQFDPSDFGPAGWSSTGPDSDELLLTSGPRHFVDCIRGDAEPVMSAEHAL
                     HVLEVMNAAELSIKSGESVELQTRFALPV"
     gene            2734..>4206
                     /locus_tag="VHR64_06360"
     CDS             2734..>4206
                     /locus_tag="VHR64_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302489.1"
                     /translation="MLAFLAIATAFGAWIGTGLLAQRRPDLRAALMTFAASNSIFAGS
                     VVTSEWAESAVSPLWPSAGAIAILVLATCLATRPHVRAASTIDDWLDLTFMIGATLFC
                     GWELLLRPLRSTNAAHVVFGLALVVAFGALAIQYNLANSRTPFAVMIPASVLLVILAP
                     GGSHVTSIFGPSPHLGLSHQVWIACAALAGTVALHGGFRYWRPTSRTWGFPQRFRNAL
                     ILDGVAILIILVAAMSVAARETSRLPAFASLSVLAMLFLARCIRAYLMIIGLEASVAT
                     RNAIIRLPGQAVTVRSDEDFVAILTQLAQSAATLVGTSRAEVHLDAPDGMSPNTRVAW
                     GLTTIESDLLRQHPAPDLSDTDREPDPDRPFLRSGTDAGLPVAAQHAWQAAGKETVLV
                     IPLLAGPHRLGIIELWSPAEPLELTPALEMTAADFGREAGVIIEHSRLVLTTRGDIVE
                     AEMLLAVSEVFSRATSLTECLPIVAQRLLKRAESDRITITL"
BASE COUNT          695 a         1477 c         1280 g          754 t
ORIGIN      
        1 tataaagccg gacagtcgta ccaatcccgg ggtgaatcgc gggtcgggag gacacatgcg
       61 caacgttgag caactgcaaa tcgagctgcg cgaacacctg cactcgctcg agcgaagcgg
      121 cgtagtcggc ctgtctgcaa cgtttcaacc ggccgagtgg gccgcgatcg tgccccgtct
      181 cgagcatgac gagctcgtta cgctcttcga ttggctccct gacgacgacg cggccacggt
      241 cctggcagcg ctcgatcccc acaccgccgc cgacgttttc cgggccatcg gcccggtcca
      301 ggccgccgcc gcgctggacg agatgcccga ggatgacgcg gccgatgtcc tcgaagctct
      361 gcccgacgag cacgccaacg cgctgctgac cgcgatacct gcggacgaag ccgaggagct
      421 gcgcgatctc ggccagttcg accccgatac ggcgggcggc atcatgtcgc ccggcttcgt
      481 cgccatggct ccgagcctgc acgccgatca ggccatcagc gccctccgca agatcgccgc
      541 cgaacggcag ccagtgacct atacctacat cctcgacgac gtgggcaggt tactcggcgt
      601 gctttcgctg catgcgctcg ttctcgccgc acccgatacg ctggtcaccg acttgatggt
      661 ccaggacgtc gtgcgcgtcc gcgctgatac tgaggcgacg gtcgcggccc agctcctgcg
      721 gacccaccgc ctgctcgcgt tgcccgtggt cagcgacgac gaccagctcc ttggcctgat
      781 cacgccggac gacgtcgcca atctcgcgct cgaagaggcg atcgaggata tcgagcgtct
      841 cggcggttcc cagccgctcg aaacgtcata ccgggccgcc tcgatcaccc tgctcgtccg
      901 ccgccgcctg ggttggctga tggtcctttt cctggccgag atgtacacga gcacggtcct
      961 caagcactac gacggcgata tcggccggtt gccgctcctg agcatcttta tcccgctcct
     1021 gaccggcgtc ggcggcaacg tcggcagcca gacagtcggt accctgatcc gctcgatggc
     1081 gatcggcgaa gtccgtcttc gcgatatccg atgggtcgtc gccaaggagt ttggaaccgc
     1141 tgccttgatg ggtctcggta tggccgtggt cgccttcggc cgggccgaat ggctcggcgt
     1201 gggtcacgat ctggcgctgg tcgtcgcgct ctcgatcatc gtcatctgcg cgtggtcggc
     1261 gaccgttgcg gccgttctgc ccctgattct ccgggccttg cgcatcgacc cggcgctcgt
     1321 ctccgcgccg ttgatcacga cactggtcga cggcaccggg ctgatgatct atttcacgat
     1381 cgccgagttc atcgtcctca ggtagcccgc gcgccggcta taccggcaac gcgaacctgg
     1441 tttgcagctc gaccgattcc ccgctcttga tcgacagctc ggcggcgttc atgacctcga
     1501 gcacgtgcag ggcatgctcg gccgacatca ccggctccgc atcgccccgg atgcaatcga
     1561 cgaaatggcg cggtccagag gtcagcagca gctcatcaga atccggaccg gtcgagctcc
     1621 agccggcggg accgaagtcg gacgggtcga actggaacag atcaaccccc tgaccgaccc
     1681 agtactccat ctctccgacg accgacgcaa tcccccgctc gccgatcacc tcgagcaccg
     1741 gcccgcggtg actcggcatt gagaagctgg aaatgatcgt cgcgtaccgg ttgtcggcaa
     1801 attccacctg catcgagatc aggtccggcg cctgcaccgt gaccttctgc ccggccaggc
     1861 gcggaatcgt gatcgtccgc tccgggatca ggatcccgcc catcgcctga acacgctggg
     1921 ctggcccgaa caagccagtc agggtcgtga tcaggtagac acccaggtcg acggccggac
     1981 ccaccccggc catatagaag acggccggat cacccgtgta ttcgttccat ccggccggcc
     2041 ccatcgtggc gagccgggcg aatgcatgcg tgacccggcc gatcgcgccc gactgaatga
     2101 cctcctggat ccactgaacg cggggcgtcg ccatcacggc gggagcacag agcagcaact
     2161 tgccgaccga ctcggcgtgc gcaatcaact cccggccgtc cgcgaccgtc gccgccagcg
     2221 gcttctcgga gaggacgtgg aaaccggccg acaagagcgc cgtcgtggtc tcgcgatgga
     2281 acggtgcggg cgtcaggttg atcacgccgt cgatatccgt ccgctcggtc aacgcggtca
     2341 gcgtcgacag tgcgtccgcg cccgggaaga gcgaggccgc ccggatcgct cgatccgcga
     2401 tcggatcgca cagggcgaca acctccaccg aatcgccgag cgtcttcagt cccgagaagt
     2461 acgagcgaaa ggccacatcc ccacaaccaa gcaccgcaag ccgaaccatc cccatgcctc
     2521 ctggtccggc gtaccaccgg tccgtacgta catcagccaa tttggatacc atcgcacagc
     2581 caccactcct acggagggcg ccccgccttt ataccttccc tcccattcta tttggataac
     2641 ccgattcgta cgctttcagt gttgtccgta tgcagatagc gctgcgcact cacgcgggcc
     2701 aaagggcgga ccgccgggag attcgctcaa gacatgttgg cattcttggc catcgcgacc
     2761 gcgttcggcg catggattgg taccggtctg ctcgcgcagc gcaggccgga cctgcgcgcg
     2821 gcgctgatga ccttcgcggc aagcaatagc atcttcgccg gatcggtcgt cacgtcggag
     2881 tgggccgaga gtgccgtctc accgctctgg ccctccgccg gcgcgattgc gattctggtg
     2941 ctggcaacct gcctggcaac ccgacctcac gtccgggccg cctccacgat cgacgattgg
     3001 cttgatctca ccttcatgat cggcgccacc ctcttctgcg ggtgggaatt gctgcttcga
     3061 ccgctgagat ccaccaacgc ggcccatgtc gtcttcggat tggcgctagt ggtcgcgttt
     3121 ggcgcgcttg cgatccagta caacctcgcc aattcgcgaa ctccgtttgc ggtgatgatc
     3181 ccggcctctg tgctgctggt catcctggcc ccgggtggga gccacgttac ctccatcttt
     3241 ggtccgtcgc cgcacctggg cctgtcccac caggtctgga tcgcatgcgc ggcgttggcc
     3301 ggcaccgtcg cactgcatgg cggattccgg tattggcggc cgacgagccg gacctggggt
     3361 tttccgcaac gattccggaa cgcgctgatt ctcgatggcg tcgcaatcct gatcatcctg
     3421 gtcgccgcca tgtcagtagc cgcgcgcgag accagccgct tgccggcatt tgccagcctg
     3481 tcggttctgg ccatgctctt cctggcacgc tgcatccggg cgtatctgat gatcatcgga
     3541 ctggaggcgt cggttgcgac ccggaatgcg atcatccgcc tgcctggaca agcggtcacg
     3601 gtccgcagcg acgaggattt cgtcgcgatc ctgacccagc tggcccagag cgccgccacg
     3661 ttggtcggca cgagccgcgc cgaagtgcac ctcgatgcgc cggacggcat gtctccaaat
     3721 acccgtgtcg cctggggtct gaccaccatc gagtccgatc tgctccggca acacccagcg
     3781 ccagacctct cggataccga tcgtgagccc gatccagatc ggccgttcct gcgatccggg
     3841 acagacgccg gattaccggt ggccgcgcag cacgcctggc aggccgccgg caaggaaact
     3901 gtcctcgtca tcccgcttct ggccggcccg cacaggctcg gcatcatcga gctctggtcg
     3961 ccggccgagc ctctggagct gactccggcg ctggagatga cagctgcgga cttcggccgg
     4021 gaagccgggg tgatcatcga gcacagccgg ctcgtcctta cgacccgcgg cgatatcgtc
     4081 gaggcagaaa tgctgctcgc ggtcagcgag gtcttctcgc gggccacgtc gctcaccgaa
     4141 tgccttccga tcgtggccca gcgcctcctc aagcgggcgg aatcggaccg catcacgatc
     4201 accctg
//
LOCUS       DATAOZ010000240         5322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5626150, whole genome shotgun sequence.
ACCESSION   DATAOZ010000240 DATAOZ010000000
VERSION     DATAOZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5626150"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VHR64_06365"
     CDS             complement(<1..404)
                     /locus_tag="VHR64_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX3302490.1"
                     /translation="MATEFAHLHLHTEYSLLDGLGRIPEYINRAHEVGIQHVAVTDHG
                     VMYASLEWYRQATKAGLHPIVGIEAYLAEGKASVRDRKSYHLLLLAENIEGYRNLLQL
                     ASKASLEGFYYRPRIDLEMLQQHKEGLIATSA"
     gene            complement(596..1006)
                     /locus_tag="VHR64_06370"
     CDS             complement(596..1006)
                     /locus_tag="VHR64_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3302491.1"
                     /translation="MSGKEFGSLLKTLRERHGVSQSKLAERADFDHSYVSRLESGARM
                     PTREAVERLGSAMGLEQGALDALLASAGFLPRDVTSLLSNEPALGEVLEMLQDESVPT
                     EYRENMRNMLRLMVQQARHMVGASGVPIKSGVAA"
     gene            1250..2233
                     /locus_tag="VHR64_06375"
     CDS             1250..2233
                     /locus_tag="VHR64_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX3302492.1"
                     /translation="MLYSRAAAAVLTRFWPVRRIPLTAQLLRGAGLSGRLRTEIDLAI
                     QRLILAGGPPPTLATILVGADPSACAYRASIERTVGRVAIAHLAVDLPITIGREDFSL
                     EIRRLSDDPAVTGILVLMPLPPHLPQSLVYENLSPLKDVDGITPVNAGRMHMGLPALR
                     PSTPQGGIELLDHYGVEIEGRRTVVIGRSNVVGRPLASLLVQRHATVTICHRRTVDLP
                     SITRQADILCVAAGHAGLVTRDMVKPGAVVLDFGVNAVGDGITGDVAFDEVSGIAGAI
                     TPVPGGTGPVTALVLARNTVAAGFASLGGTLDAVQELTRVVAELGPRPSHG"
     gene            2291..2361
                     /locus_tag="VHR64_06380"
     tRNA            2291..2361
                     /locus_tag="VHR64_06380"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2323..2325,aa:Gln,seq:ctg)
     gene            complement(2488..4086)
                     /locus_tag="VHR64_06385"
     CDS             complement(2488..4086)
                     /locus_tag="VHR64_06385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302493.1"
                     /translation="MTATASVSSTKEAAGNQKLAILLAMAMFVLVIDTSMMNVSISAV
                     VKDLGTTVSGVQSAIALEALVSAAFILINSKVGDLIGRKRAYVIGLIGYAIGALTMAV
                     TQSLTWVIIFWAIFGGIGASLLLPAMQSLIHGNFEGKAQAKAYAMVGAAAAIAAAVGP
                     LLGGFITTYLSWRVGFLMEVVIIAIVLSGINLVQDVPYEGQRGVDIVGAVLSVLGMGG
                     VVLGILVWQEGGEAVGALIAIGVIALASLAYWLIRRKREQKLILLDPDLFTSPHFRIG
                     ISQQMLQQISLGGTMIALPIFLQMVLEYNAMKAGLSMAPLSLSMFVIAVLAGKKAGKR
                     RPSTLIQAGFALLTIGMAALILVVPRADSGWYLTIPLMIAGVGLGMLVSQLNNFTLAP
                     ITEDRISEAAGVNSAAGNFGLSFGLAFAGAIMLATLSFLFTSMAEDSSVLTPTEQQKV
                     ADVLEHDAQVMSNTQLEEQLASQPPATQDEIIRINTDARPRALQVALLVPLIAGFLGL
                     GLSFRMARLPEITPSASLEGVLGA"
     gene            complement(4357..>5322)
                     /gene="recF"
                     /locus_tag="VHR64_06390"
     CDS             complement(4357..>5322)
                     /gene="recF"
                     /locus_tag="VHR64_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX3302494.1"
                     /translation="VVGIVDSAEGELTIELGLQRSDEPGGPLRKIVRINQQPRRVLDA
                     VGALKTVLFTPEDLELVNGSPQGRRRFMDLLIGQIDRRYVSELSAYQKVTEQRNSLLK
                     SFARGTVSRHNAIEQLGFWDDQLAEHGSYVSASRRIMIGRLCAAFTERSRLFTASAML
                     TARYAPNLGADFLLAGRADDDPDQVRAVALREFQTLIRIRRDDELRRGVTLIGPHRDD
                     LELLIDERPLASFGSRGQQRLAVVALKLAEADVISMLGDRPVLLLDDVLSELDASHRE
                     TLLASVRVFGSQVLLTTADPVQVAGSPVADLEIIQVIPGGVDFPA"
BASE COUNT         1003 a         1703 c         1652 g          964 t
ORIGIN      
        1 caggccgacg tcgcgatcaa cccctccttg tgctgttgca gcatctcgag gtcgattcgc
       61 ggccgatagt agaacccttc aaggctcgcc ttggacgcga gctggagcag gtttcgatac
      121 ccttcgatat tctcggccag cagaagcagg tggtacgact tgcggtcccg gaccgatgcc
      181 ttgccctcgg ccaggtacgc ctcgatgccg acgattgggt gcagccccgc tttggttgcc
      241 tgccggtacc actccagcga cgcatacatg acgccgtgat cggtcaccgc aacatgctgg
      301 ataccgacct catgcgcccg attgatgtac tcggggattc gcccgagtcc atcgagcagc
      361 gagtactcag tatggagatg tagatgcgcg aactcggtcg ccacggcagg gtactccggc
      421 actgatcttg gggaatccgg gattgctcgc cggctcccta atcgtattgc gaaacggcca
      481 aacgctcaac gggcgggatt ggtgcgggcg ggcctggaaa cagtgatccc tggtccggtg
      541 cgggcgggaa tcggaccagg gatcgaagac gctttcagat tgaaatgata acgacctagg
      601 cggcaactcc gctcttgatc gggacgccgg acgcgccaac catgtggcga gcctgctgaa
      661 ccatgaggcg gagcatgttc cgcatgttct cgcggtactc ggtcgggacg ctctcgtcct
      721 ggagcatctc gagcacctca ccgagcgccg gctcgttcga cagcaagctg gtgacatcgc
      781 gcggcagaaa cccggccgac gccagcaggg catcgagtgc accctgctcc agccccattg
      841 ccgaacccag acgttcgacc gcctcgcgag tcggcattcg ggcgccgctc tccagccgcg
      901 aaacgtagct gtgatcgaaa tcggcgcgtt ccgccaactt gctctgggag actccgtgtc
      961 gctcccgaag cgtcttcagg agtgatccga actccttgcc gctcatccaa acacctgtga
     1021 ctttctggca ccgatgtgcc gatcctcgcc cgcaagtcac aagggaggtg acttgtggac
     1081 aagatcgtag caactccatg ccccggtgtc aattcccctg tcacttttcc tgtcgcaacg
     1141 ccagcatatt gccatcactg gcgataccaa tggcactggc cgaagcgcgg acgctccgct
     1201 gtggttagct gtcattaacc cggaaattca ggggtatgtc gggagtcgat tgctatactc
     1261 tcgtgcggct gcggccgttc tgacgcgatt ctggccagtc cggagaattc ccctgacagc
     1321 tcaactcctg cgcggcgccg gtctttccgg ccggcttaga acagagatcg acctcgccat
     1381 ccagcgactc atcctcgccg gtggaccacc accaaccctt gccaccatcc tggtcggagc
     1441 cgacccctcg gcctgcgcat accgggcgtc gatcgaacgg acggttggcc gggtcgcaat
     1501 tgcccacctc gcggttgatc ttccaattac gattggtcgc gaggatttca gcctcgagat
     1561 ccgccggttg agcgacgacc cggcagtcac cgggatcctg gtgctgatgc cgctgccacc
     1621 gcacctgccg caatcgctgg tgtacgagaa cctcagcccg ctcaaggacg tcgatggcat
     1681 cacgccggtc aatgccggcc ggatgcacat gggtctgccg gcgctccggc cgagcacgcc
     1741 acagggcgga atcgaactcc tcgaccatta cggggtcgag atcgagggcc ggcgcaccgt
     1801 ggtgatcggc cgcagcaacg ttgtgggtcg tcccctcgcc tcgctcctgg tccagcgcca
     1861 tgccacagtc acgatctgcc atcggcgcac cgtcgacctg ccgtcaatca ctcgccaggc
     1921 cgacatcctc tgtgtggcag caggtcacgc cggcctggtc acccgtgaca tggtcaaacc
     1981 cggcgcggtt gtgctggatt tcggcgtcaa cgcggtgggc gacggcatca ccggggatgt
     2041 cgcctttgac gaagtgtccg ggatcgccgg cgcgatcacc ccggtcccgg gcggaaccgg
     2101 tccggtcacg gcgctcgtcc tggcccgcaa tacggtcgct gccggctttg catcgctggg
     2161 cggaacgctg gatgcggtgc aagaactgac ccgggtcgtc gccgagctcg gaccccgtcc
     2221 gagccacggg tagacgcccg ggattacagg gcgtgcgaat ggccagatgt gctatctttt
     2281 gttgccgcga tggggtgtcg tctaacggta ggacgcctga ctctggatca ggtagttggg
     2341 gttcgaatcc ctgcacccca gccccaaact aaccacaagg aatgcatgaa ggcgagccgg
     2401 gaggtctgtt ccacccggct cgaattgcat ctttgccgcg ctacgatttt ggggtcgtgt
     2461 ctatccgtcg tgccaaggcc cgacaactca ggcgccgagg accccttcga gcgatgcgct
     2521 cggcgtaatc tcgggcaggc gcgccatccg gaacgacaac ccaaggccaa ggaatccggc
     2581 aatcagcgga accaggagcg caacctggag cgcgcgcggc cgggcatcgg tgttgatccg
     2641 aatgatctcg tcctgcgtgg ccggcggctg actggcgagc tgctcctcga gctgggtgtt
     2701 gctcatcact tgcgcatcgt gctccaggac atcggcgacc ttctgctgct ccgtcggcgt
     2761 gagcacgctg ctgtcctcgg ccatgctggt gaacaggaat gaaagagttg ccagcatgat
     2821 cgcgccggca aacgccagac cgaatgaaag tccgaagttc ccggccgcgg aattcacccc
     2881 cgccgcctca ctgatccgat cctccgtaat tggcgcaagg gtgaagttgt tgagctgcga
     2941 gaccagcatg cccaggccca cgcccgcgat catgagtggg atcgtgaggt accacccgga
     3001 gtccgcccgg ggcaccacca ggatcagcgc ggccatacca atggtcagca gggcgaaacc
     3061 agcctggatt agcgtgctcg gtcgccgttt tccggccttc ttgcccgcca gcacagcgat
     3121 gacaaacatg gaaagcgaaa gcggcgccat cgaaagcccc gccttcatgg cgttgtactc
     3181 gagaaccatc tgaaggaaga tcggaagggc gatcatcgtc ccgcccagcg aaatctgctg
     3241 cagcatctgc tgcgagatgc caatccggaa atgcggcgag gtgaagagat cggggtcgag
     3301 caagatcagt ttctgttcgc gcttgcgccg gatcagccag tatgcgaggg acgccagcgc
     3361 gatgacgccg atcgcgatca acgcgcctac cgcctcgccg ccttcctgcc agaccagaat
     3421 gcctagcacc acgccgccca tgcccaggac tgagaggact gcgccaacga tatcgactcc
     3481 ccgctgtcct tcatatggca cgtcctggac caggttgatg cccgaaagta cgatcgcgat
     3541 gatcacgact tccatcagaa agccgacgcg ccaggacagg taggtcgtga tgaagccgcc
     3601 aagaagcgga ccaacggcag cggcgatggc tgcggcagcc ccgaccatcg cgtatgcctt
     3661 cgcctgcgcc tttccttcaa agttgccgtg gatcagcgac tgcatggcgg gcaacaggag
     3721 tgaggcgcca attccgccga agatcgccca gaagatgatg acccaggtca ggctctgggt
     3781 aactgccatg gtgagcgcgc cgatggcata accgatcagc ccgatcacat aggctcgctt
     3841 gcgaccgatg aggtcgccta ctttgctgtt gatcagaatg aacgcggcgg aaacaagtgc
     3901 ctcgagtgca atggcggatt ggacgccgct cacggtcgtc ccgagatcct tgaccactgc
     3961 cgagatcgag acgttcatca tcgatgtgtc gatcaccagc acgaacatcg ccattgccag
     4021 cagaatggcc agcttctggt tgcccgcagc ttctttggta ctgctgacgc tcgcggttgc
     4081 cgtcatcgga tgtcctccga tcgctggatc gatccaggag tggtgagcgc gcggtttgtc
     4141 gctcaccgcg cacggttcag ctgcaacgag aaggtgaggt gccgacgtgt tggaagcccc
     4201 gcctgcggct ggcaggcggc aacgtggcca acggccattg ccgaaggcat ggtggccggt
     4261 tgagggtcgt aatcgcatcg gtccaatggc gcaatcgtcc ggaagcgatc agagaccgcc
     4321 cagggcaatt gccgtgatca tcctggcagc ttgggttcag gctgggaagt cgactccgcc
     4381 tggaatgacc tggattatct cgaggtccgc gaccggactt ccggccacct ggaccggatc
     4441 ggcggtggtc agcaggacct gcgagccgaa cacccgcacc gacgccagca gcgtctcccg
     4501 gtggctggca tcgagctcgc tgaggacgtc atcgagcaac agcaccggcc ggtcgccaag
     4561 catcgaaatc acgtcggcct ccgcgagctt cagcgcgacc acggccagcc gctgctgacc
     4621 acgcgatcca aacgacgcca gcggccgctc gtcgatcagc agctcgaggt cgtcccggtg
     4681 tggtccgatc agcgtcacac ccctgcgcag ctcgtcgtct cgccggatcc ggatcaaggt
     4741 ctggaactcg cgcagggcga ctgcgcggac ctgatccggg tcatcgtcgg cgcgaccggc
     4801 caacaggaag tcggcgccga gattcggtgc atatcgcgcg gtcagcatcg ccgacgcggt
     4861 aaacaaccgg gagcgctcgg tgaaggcagc gcagagccgg ccgatcatga tccgtcgtga
     4921 ggcggagacg tatgacccgt gctccgcgag ttggtcatcc caaaagccga gctgttcaat
     4981 cgcgttgtgc cgggaaaccg tgccgcgcgc gaatgacttg agcaggctat ttcgctgctc
     5041 ggtcaccttt tgatatgcgg agagctcgga aacatagcgc cgatcgatct ggccgatgag
     5101 gagatccatg aagcgacggc ggccctgcgg tgatccattg acgagttcga gatcctccgg
     5161 cgtgaaaagg acggtcttga gcgctccgac cgcgtcgagc acgcggcgcg gttgctggtt
     5221 gattcgcacg atcttgcgga gcggtccgcc gggctcatcg gagcgttgca gacccagttc
     5281 gatcgtgagc tcgccttctg cggagtcgac gatgccaacc ac
//
LOCUS       DATAOZ010000241         5967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7467954, whole genome shotgun sequence.
ACCESSION   DATAOZ010000241 DATAOZ010000000
VERSION     DATAOZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7467954"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VHR64_06395"
     CDS             complement(<1..246)
                     /locus_tag="VHR64_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HEX3302495.1"
                     /translation="MDQLSASRIPNHVAIIMDGNGRWAAQRGLPRLAGHEAGTENIRR
                     ITFKAGELGVKYLTLWAFSTENWRRPQEEVQGIMKILA"
     gene            complement(276..833)
                     /gene="frr"
                     /locus_tag="VHR64_06400"
     CDS             complement(276..833)
                     /gene="frr"
                     /locus_tag="VHR64_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX3302496.1"
                     /translation="MMKDAIAHAEERMKKAMEALRRDLSTIRTGRASPALLDRVHVEY
                     YGTDTPLNQVAGVSAPEARMLMIQPWDRGTIPAIEKAIQKSDLGLTPSNDGQVIRLSI
                     PPLTEERRKQLVKTVHGMVEESKVAVRNVRRDAVHSIHKLMTDKQISEDEERRGSAQI
                     DDLARHYTDQAASIGKDKEREVMED"
     gene            complement(830..1603)
                     /gene="pyrH"
                     /locus_tag="VHR64_06405"
     CDS             complement(830..1603)
                     /gene="pyrH"
                     /locus_tag="VHR64_06405"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX3302497.1"
                     /translation="MSPYRRVLLKLSGEALMGEAPYGIDPTVAKSIAAQIKRVLEHDI
                     QIAVVIGGGNIWRGLAASSKGMDRATADYMGMVATVLNALALQDALEKANVFTRVMTA
                     IQMHEVAEPYIRRRAIRHMEKGRVVILAAGTGNPYFTTDTAAALRALELDVDVLLMAK
                     HGVDGVYSDDPRTNPEARRFRQVNHQDAIERNLRVMDTAALSLCRENGLPILVFDMTA
                     DASIYAAVTGAAVGTLVNSGETIIDDAVVAVVTESESPR"
     gene            complement(1635..2195)
                     /gene="tsf"
                     /locus_tag="VHR64_06410"
     CDS             complement(1635..2195)
                     /gene="tsf"
                     /locus_tag="VHR64_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX3302498.1"
                     /translation="MVTTSLVKELREQTGAGIMECKRALEEAGGDLKKAVDLLRQTGL
                     AKADKKLGRAALQGLIETYVHAGGRIASIVELNCETDFVARTPDFKTLAREIAMQVAA
                     MNPRYVRASSVPEQDWASLESEFGDRKKATEAVCLYEQAFIRDPKVSIEELIREAVAK
                     LGENIQVQRFARFEVGADRAGADSEE"
     gene            complement(2232..3101)
                     /gene="rpsB"
                     /locus_tag="VHR64_06415"
     CDS             complement(2232..3101)
                     /gene="rpsB"
                     /locus_tag="VHR64_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX3302499.1"
                     /translation="MVASIYNESPRTVMKALLEAGVHFGHQKKRWNPKMRNYIFTEKN
                     GIHIIDLNQTVNLLNDAYQFVTNVTASGGRILFVGTKKQAQEVVAREAARSGQFFVNR
                     RWLGGTLTNFVTIRSRLRTLKQLQQESATGGFERLPNQEAAAKRQELERLERTLGGMR
                     DMTSLPGAIFVIDPKREHLAIQEARRLKIPVIAITDTNCDPDLVDYIIPGNDDAIRSV
                     RLITAAIADAAIQGSMQAEIAVAESEMDMGEAEPDAASMAIEAAATAEVPAEAETTEE
                     AEAPVATEESATA"
     gene            complement(3267..3863)
                     /locus_tag="VHR64_06420"
     CDS             complement(3267..3863)
                     /locus_tag="VHR64_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3302500.1"
                     /translation="MSYESEERYWTDYLRVALPVIGLLLMLALFWWWAQQFIGDDKDG
                     NQVAMEATQTTEIATKAAPTPTNTQEVVVQPTQVETTPTSETSGTDGNTGNNGGQAEN
                     TPADNSGGNTGQNCDFKKGDYVVVTQEGDGLRLRTDPEQSDSNIVDTLDAGTVLRVVE
                     DCTVEDADGNQYVRVRDEATSRTGYVSADFVEPQPNDG"
     gene            complement(4248..4835)
                     /locus_tag="VHR64_06425"
     CDS             complement(4248..4835)
                     /locus_tag="VHR64_06425"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX3302501.1"
                     /translation="MSMRMPASNEPGYRAPTPEGDSDTFSVIDRNSIFDGTFQTTRDL
                     QVEGEVKGTIDCKGTLFVAGGAVVGAKVEAENVTVAGDLNGEVRCRGRLQIMPTGRVR
                     GKIVTQTLVINEGAFYEGQLEMAPPESRTPAQPARPVQQQEQKPPVAISTAASRAAAT
                     EQQSGSTFIRRLGGPETPWERQDEDETERASEESQ"
     gene            complement(4863..5474)
                     /locus_tag="VHR64_06430"
     CDS             complement(4863..5474)
                     /locus_tag="VHR64_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX3302502.1"
                     /translation="MFRRDNKGDSFERQMGALRQQLGDTDVEEDEDLLEEQQLDAEDD
                     YADRARAYGFGGQQQQSFTTPAQPPAAPMIPDVDPSTTVVSRDTTWKGEMSSEGSVHI
                     HGRFDGAVKATQAIFVAEGAEVDATMTAETIIVAGVSRGTVRARGKFEVLPSGRVAGD
                     IHAPTLVVHEGAIMTGQLRMSGDEPADAKPTPVIQRRPSRGTA"
BASE COUNT         1010 a         1859 c         1842 g         1256 t
ORIGIN      
        1 ggccaggatc ttcatgatgc cctggacctc ttcttgcggc cgtcgccagt tctcggtcga
       61 gaatgcccac agcgtcaggt atttgacccc gagttcgccg gccttgaacg tgatccgtcg
      121 gatgttctcg gtgcccgctt cgtgacctgc gaggcgggga agtccacgct gcgcagccca
      181 gcggccgttg ccgtccatga tgatggcaac gtgattggga atccgactgg cggaaagctg
      241 atccatagct gtgtgggtga acgacgggat ggaggctagt cctccatcac ctcgcgctcc
      301 ttgtccttgc cgatcgaggc ggcctggtcc gtgtagtgcc tggcgagatc atcgatctgt
      361 gctgatccgc gccgctcctc atcttcggag atctgcttgt cggtcatgag cttgtggatc
      421 gaatggactg cgtcccggcg gacattgcga accgcaacct tcgactcctc gaccatgccg
      481 tgcacggtct tgacgagctg cttgcggcgt tcctcggtca agggcgggat cgaaaggcgg
      541 atgacctgcc cgtcgttact gggcgtcaag cccaggtccg acttctggat cgccttttcg
      601 atagcaggga tcgtgccgcg gtcccacggc tggatcatca gcatccgggc ttccggagca
      661 gaaacgccag cgacctggtt cagcggagtg tcggtgccgt agtactcaac gtggacacga
      721 tcaaggagcg ccggcgacgc gcgaccagtg cgaatggtcg agaggtcacg gcgcagggcc
      781 tccatggcct tcttcatccg ctcttccgcg tgtgcgatgg cgtccttcat catcgagggc
      841 tctcactttc tgtcactacc gcgactaccg catcatcgat gatagtctcg ccggaattga
      901 ctaatgtgcc aactgcggca cccgtgacgg ccgcgtagat cgacgcatcg gcagtcatat
      961 cgaagacgag aatcggcagc ccattctcgc ggcagagcga tagcgcagcg gtgtccatta
     1021 ctctcaggtt acgctctatg gcatcctggt ggttcacctg tcggaagcgc cgcgcctccg
     1081 gattcgtgcg cgggtcgtcc gaataaacac cgtcgacgcc gtgcttcgcc atcagcagga
     1141 catccacgtc cagctcgagc gcgcgcaggg ccgcggccgt atcggtggtg aagtaggggt
     1201 tgccggtgcc agctgccagg atgacgacgc gccccttctc catgtgccgg atcgcgcggc
     1261 gccgaatgta tggctcggcg acctcgtgca tctggatcgc ggtcatcaca cgggtaaaga
     1321 cgttggcttt ctcgagcgca tcctgcagtg caagcgcgtt caggaccgtg gccaccatgc
     1381 ccatgtagtc ggcggtggcc cggtccatgc ccttgctgct cgccgccagg ccgcgccaga
     1441 tattgccgcc gccgatgacg acggcaatct ggatatcatg ttcgagtacg cgcttgatct
     1501 gggccgcgat cgacttcgcg acagtcgggt cgattccgta tggcgcctcg cccatgaggg
     1561 cttcgccgct gagtttgagc aagacacggc gatacggcga catacccgga ttcccttcag
     1621 aatgaagatc gaacttactc ttcgctgtcg gcccccgcgc gatcggctcc gacctcgaac
     1681 cgggcgaatc gctgcacctg gatgttctcg ccaagcttcg cgacggcctc gcggatcaac
     1741 tcctcgattg agaccttcgg atcacgaatg aaggcctgct catacaaaca tactgcctcg
     1801 gtggccttct tgcgatcacc gaactcggat tcgagcgacg cccaatcctg ctccggaaca
     1861 gagctggccc gtacatagcg gggattcatg gcggcgacct gcatggcgat ctcgcgcgcc
     1921 agcgttttga aatccggagt gcgcgccacg aagtcggtct cgcaattgag ctcgacgatc
     1981 gaggcgatgc ggccgccggc atgaacgtat gtctcgatca gaccctgcag ggcagcccgg
     2041 ccaagcttct tgtcagcctt ggccaatcct gtctggcgga gaaggtcgac ggccttcttg
     2101 agatcgccgc ccgcttcctc gagtgctcgc ttgcactcca tgatgccggc gccagtctgc
     2161 tcgcgcagtt ccttaacgag agaggttgta accactgatc gtgtcttcct tccagattga
     2221 tttcgtacgc gttacgccgt agcggactct tcggtcgcga ccggcgcttc ggcttcctca
     2281 gtcgtttcgg cctcggcagg gacctcggcc gtcgcggcgg cctcaatcgc catgctcgcc
     2341 gcatccggct cggcctcgcc catgtccatc tcggactcgg cgacggcaat ttcggcctgc
     2401 atcgagcctt ggatcgcggc gtcggcgatt gcggcggtga tcaggcggac cgagcgaatc
     2461 gcgtcatcgt tgcccgggat gatgtagtcg acaaggtccg gatcgcagtt ggtgtcggta
     2521 atggcgatga ccgggatctt caggcggcgg gcctcctgga tggccaggtg ctcgcgcttg
     2581 ggatcgatta cgaaaatcgc gcccggcagg ctggtcatgt cacgcatgcc gccgagggtg
     2641 cgctccagcc gctcgagctc ctgccgcttg gccgcggcct cctgattcgg gagccgttcg
     2701 aagccgccgg tggccgactc ctgctggagc tgcttgaggg tgcgcaggcg ggagcggatc
     2761 gtgacgaagt tggtcagcgt accgcccagc cagcgccgat tgacaaagaa ctgcccagag
     2821 cgcgcggcct cgcgggcgac gacttcctgg gcctgcttct tggtgccgac gaaaagaatg
     2881 cgaccgcccg aagccgtgac gttggtcacg aactggtagg cgtcgttgag cagattgacg
     2941 gtctggttga gatcgatgat gtggatgccg ttcttctcgg tgaagatgta attgcgcatc
     3001 ttcgggttcc agcgcttctt ctggtgtccg aagtgaacgc cggcctcgag aagggccttc
     3061 atcaccgtac gcggcgactc gttgtagatg gacgcgacca cgacaaacct cctgcggttt
     3121 gatgcttccg ctttcgtcat cccgcgccgg actccggaat tgcccgaagc accgccggcg
     3181 tcagtcggaa agcgtgccaa ataaacgccc ggcagcgcgc cgggcgtctg acagtatatc
     3241 agttcgaacg aggttccggc gcgaatttac ccgtcgttgg gttggggctc gacgaaatcg
     3301 gccgacacgt agccggtgcg ggatgtcgcc tcatcccgaa cccgcacgta ctgattaccg
     3361 tcggcgtcct caacggtgca gtcctcgacg acacgcaata cagtgcctgc atccaatgtg
     3421 tctacgatgt tcgagtcgga ttgctcagga tcagtccgaa ggcgtaatcc atcgccctcc
     3481 tgggtcacga cgacgtaatc gcctttcttg aaatcgcagt tttgaccagt gttgccgccg
     3541 gaattgtcag caggcgtgtt ctctgcctgg ccaccgttgt taccggtatt gccatcggtt
     3601 ccggaagtct ccgaggtcgg cgtggtctcg acttgagttg gctggacgac gacttcctgc
     3661 gtgtttgtcg gagtcggcgc cgccttggtc gcgatctcgg tcgtctgggt cgcttccatg
     3721 gcgacctggt tgccgtcctt gtcgtcgccg atgaactgct gcgcccacca ccagaagagg
     3781 gcaagcatca gcaacagacc gatcaccggc aaggcgaccc ggagatagtc tgtccagtag
     3841 cgctcttcgc tctcgtagct cacggtacgc tcctgaccgg tcggtgcgcc cgaatcccag
     3901 ctgcgggttc ggcgcccagg cgtcggtgca gatccctcgc cgcggagatc actcatgcgc
     3961 atccccaatt cgcggtgcac gcatggagag ccgggagcgg acgagtcgcg cggcgacgat
     4021 agtcttgcat tggtaacggt ctgatggaat cacaggtttc gtttatttgg ccggggaggg
     4081 tgggttggag tgtcagacga gtctcgattt cccccaccag cgaatcccca cttttgacca
     4141 gattccccac agtggccgga gtatagcacg gaccctgtcg cgcccatcac gaaacgcgcc
     4201 ggtccacatc tggaccggcg cgctgaaatc gttgatttgt tggtgggcta ctggctctct
     4261 tcagaggcgc gttccgtctc gtcctcgtcc tgtcgttccc agggagtttc tggcccgcca
     4321 aggcgacgga tgaacgtgct gcctgactgc tgctcggttg cggcggcgcg gctggcggcc
     4381 gtcgagatcg ccacgggcgg cttctgctcc tgctgctgga ccgggcgagc aggttgggcc
     4441 ggcgtgcgac tttccggcgg cgccatctcg agctggcctt cgtagaacgc gccttcgttg
     4501 ataaccagcg tttgggtcac gatcttgccg cggactcttc cggtcggcat gatctggagg
     4561 cggccacggc agcgaacttc gccgttcagg tcgccggcaa ccgttacatt ctcggcctcg
     4621 actttggctc cgacgaccgc accgccggcc acgaagagcg tgcccttgca gtcaatcgtc
     4681 cccttgacct cgccctcgac ctgcaggtcg cgagtggtct ggaacgtgcc gtcgaaaatc
     4741 gagttgcgat cgatcacgct gaaggtatca gagtcgccct ccggcgtcgg agcacggtaa
     4801 cccggctcat tgctggctgg catgcgcatc gacatgacag aagatccttt cttcaggaat
     4861 tgctaggcgg tgccgcggct cgggcggcgc tggatgacag gagtcggctt ggcatccgcc
     4921 ggctcgtcgc cggacatccg gagctggccg gtcatgatcg ccccttcatg gacgaccagc
     4981 gtcggcgcgt ggatatcacc ggcaacacgg cccgacggca gtacctcgaa ttttccgcgt
     5041 gcccggaccg tgccgcgaga gacaccagcc acgatgatcg tctccgcggt catcgtcgca
     5101 tcgacttcgg ctccttcggc cacgaagatg gcctgggtgg ccttgacggc gccatcgaac
     5161 cgcccgtgga tgtggacgct cccctccgag ctcatttcgc ccttccaggt cgtgtcccgg
     5221 gagaccacgg tcgtcgaggg gtcgacatcc gggatcatcg gcgcggccgg tggctgggcc
     5281 ggggtggtaa aggactgctg ctgctgaccg ccgaagccgt aggcgcgggc gcgatcggca
     5341 tagtcgtcct cggcatcgag ctgttgttcc tcgagcagat cctcatcttc ttcgacgtcg
     5401 gtatctccaa gctgttggcg aagcgcgccc atttgccgct cgaagctgtc gcccttgtta
     5461 tcgcgtctga acacgtccga acctctcctt ctcaacgccg gggagtctcg ctcacgcgcc
     5521 gttgcggtag cgcatgtcgg gatagcgagc gtcgtccagt catggcgtac ttacgcaaat
     5581 ctggagcgta cgtacagtct atcaccggtc gtcaaggtga aagcatcccg caaaagtcct
     5641 taataagaca tacggtatgc gtgccgatca taacgctcaa acgagcatcg gcgtttcagt
     5701 ctcgtactgg ttgcattttg acataagtga ctatatgcgg tttgtagccg tcatgttccg
     5761 gccggacgcg cgggcttcct gcctgagtta ccgacctgaa tcgttatcca ggccggcctc
     5821 gaatgcagcg cacatccgtc tccgcggatt gcctgaacct tggcatcgat cctgcaatct
     5881 cgtgccgaag gtcaattagg ccagaattca gagagatata gatatcggat tcgaccatat
     5941 cttcctgcat aatgtgaaca gagagca
//
LOCUS       DATAOZ010000242         6704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3784319, whole genome shotgun sequence.
ACCESSION   DATAOZ010000242 DATAOZ010000000
VERSION     DATAOZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3784319"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VHR64_06435"
     CDS             complement(<1..725)
                     /locus_tag="VHR64_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3302503.1"
                     /translation="MVRFCANLSMLFVEAPFLERFGRARAAGFEAVEFQFPYDFAPDD
                     IKSELDRHNLELVLFNLPAGDFAAGDRGMANDPARVDDFRATLGTAMEYARVLKPAKM
                     NCMAGKQLADVPVDKQRATLIANLRDAAEQAAAKGILLVTEPLNPHDAPGFFLPRPSD
                     GFAIVAEAHHANLKVEYDIYHAQRTEGEIVATIRDHIDAIGHIQIADNPGRNEPGTGE
                     LNWPFIFAAIDEVGYDTRIGLEY"
     gene            817..2724
                     /locus_tag="VHR64_06440"
     CDS             817..2724
                     /locus_tag="VHR64_06440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX3302504.1"
                     /translation="MTEPHILTAEELADRRYPSDIAISADGRFVAFVVQPMGKREEHD
                     LTEIWLARDGEPARQFTSGLAGDSSPVFSSDSAKLAFLSDRHEREKQRVYVMPLDGGE
                     AVRVSTVEGKLSALTWSPDGAHLAVLVTDPETETEKKQKEERNDPILENQNRKYARLW
                     SINADTGAARQLTFGERHVLEYGWSRDGRTLAVATSTRTDVEAEFQEIEISLVAVLGG
                     RLRLLATVRGLPSNLIFVDHSDGEQIAMRHNGFTDDPVDSVWLMSTATGEHRELLTDD
                     HGNVDAIMPIPGRPGQIGMRMVDGVHANAWVVDVATGERTKALAAGWQDRGTLHAGPA
                     YSADGAKIAFVWTDSVTPEEAFVAESGAEPVKLTCFGNSFAGRLNRSELVEWQSDEWT
                     IQGHLVYPHGYEEGKRYPLLIEAHGGPSWQWEDHCFLDWHDWAQFMASRGFAVLAPNP
                     RGSTGRGEAFQKALQNDVGGGEVRDLITGAQAMVERGIADPDRLGIGGWSWGGYLTAT
                     TITRTNMFKAAMMGAGLSNLFSDHGTDDIPSANLLYFPGEPYHHADMYWEGSALRCVS
                     HCTTPTLILHGDADERVHPLQGREMYTALSRLGIDTEFVRYPRQGHTIKERNYQIDLL
                     NRLAAWFEKYL"
     gene            complement(2935..4122)
                     /locus_tag="VHR64_06445"
     CDS             complement(2935..4122)
                     /locus_tag="VHR64_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEX3302505.1"
                     /translation="MTSIVDRIDRDTVLPIARSLIAIPSVSGDEAAIMEWLMTWCRER
                     NLDVTVIERDERRPNLIISIGDPSAGPTLAMNGHLDVVPVSDADAWKTGPFDPVISED
                     GVRLFGRGSSDMKASVGVMLAMMELFRDRPMRGCLQAHIVSDEEMSGHYGTVAVMDAI
                     ADGTLPKPTWCLIGEKSDLKVRNAERGILHIRVKVLGRASHTAAARTTGVNAIALAAR
                     AILALERDIDRHHPSVGKPVISINRISGGVADNVVPGECEFLIDRRLIPGETRESVVD
                     EITETLMNVAAPERGEFRFSLTVDPGGDFIPANITEPDSPLVHAVWDATRDVRGEEPE
                     FFVQWAGATDGRYYRLAGIDTVGMGPGGENAHGANEAVYIDDLVAQAKIYALTIERLL
                     SVD"
     gene            complement(4142..4471)
                     /locus_tag="VHR64_06450"
     CDS             complement(4142..4471)
                     /locus_tag="VHR64_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX3302506.1"
                     /translation="MKLVIAIVQDEDVDALTEELISAGHRFTKVSSTGSFLRTGNTSL
                     LIGVEDQYVDSIMRILERTCRRRTQVAVPYSPALEPGLLYLPENIEVEVGGAIVFVMN
                     VNRFERL"
     gene            complement(4568..5431)
                     /locus_tag="VHR64_06455"
     CDS             complement(4568..5431)
                     /locus_tag="VHR64_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX3302507.1"
                     /translation="MPPIDSAPPTLNGHEEPFSYSEEPMRDERKRYTTQLQRRAQAHI
                     TAELGGTTVVPDTDRQVTMVIQHAQNATGARRVSFFRPVARGRRWHVATVLEDGAFYY
                     GLACSETLGWSRVSFEQRRPVLYDSRDRRPETGPSLADLGLRSYLGVPVLADGRAVAV
                     IEAVDVNQTGDLEHYSAQLEETASTIAQRLDDQNKQVDEVQARPGSGDLELSADAIVD
                     LVLRQPYEVDETFEVSPAEWSVLNGVNGDRPLRAVAEQASMPISQVLSTSAALLERGL
                     IRLGKENRRRL"
     gene            complement(5473..5697)
                     /locus_tag="VHR64_06460"
     CDS             complement(5473..5697)
                     /locus_tag="VHR64_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX3302508.1"
                     /translation="MRAYVLITIEAGKGSEIQKALQEAGIGQVDQLAGIYDLVAVIDA
                     TDPRKIGEIVIGTIQKTPGVVSTVTLLTIG"
     gene            complement(5737..6192)
                     /locus_tag="VHR64_06465"
     CDS             complement(5737..6192)
                     /locus_tag="VHR64_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302509.1"
                     /translation="MDDPQSHDEAEDFEARLRELLQTAEVMSILFTRVSQSLIIDFRQ
                     TENVGPRVMTDEIVASAHDRFLSFGRLRPHLPLPEQLTLAFWTPGVREFRDIGMYTIL
                     AERCDAVGGKALVAEAQDSYRFLLKLERQYLCDMVRGVGMKTLWERARE"
     gene            complement(6196..>6704)
                     /locus_tag="VHR64_06470"
     CDS             complement(6196..>6704)
                     /locus_tag="VHR64_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEX3302510.1"
                     /translation="VSIAVVHQGVGEIGVVFDPSRDELFAARRGGRATLNGRPIQVST
                     VDVMSRSLIGTGFSYEQQQQVEQHEIWRHLQGKCQGIRRDGSAALGVAWVAAGRTDGF
                     YERPINAWDVGAGSIIVQAAGGAVSAIDGSPYDVFGQELAASNGLIHAELIDEIGAAI
                     SNVGTPVV"
BASE COUNT         1229 a         2204 c         2056 g         1215 t
ORIGIN      
        1 ttgtactcga gcccgatccg ggtgtcgtag ccaacctcgt cgatggccgc aaagatgaac
       61 ggccaattga gctcaccggt gccgggctcg ttccgtccgg ggttgtctgc gatttgaata
      121 tgcccgattg cgtcgatgtg gtcgcggatc gtagcgacga tctcaccttc ggtgcgttga
      181 gcgtgataga tgtcgtactc gaccttaagg ttcgcgtgat gcgcctcggc cacgatcgca
      241 aagccatcgc tcggccgagg caggaagaag ccgggcgcat cgtgcggatt gagaggctca
      301 gtaaccagga ggatgccttt ggcggctgcc tgctcggcag catcacgcaa attggcgatc
      361 aatgtcgcgc gctgtttgtc taccgggacg tcagccagct gtttgccggc catgcagttc
      421 atcttggccg gcttcaacac gcgcgcatat tccatcgcgg tgcccagcgt tgcgcggaag
      481 tcatccaccc gggccggatc attggccatg ccccgatcgc cggccgcgaa atcgcccgcc
      541 ggcaggttga ataggaccaa ctccaggttg tgccgatcaa gctccgactt gatgtcatcg
      601 ggcgcgaagt cgtacggaaa ctggaactcg actgcctcga atcctgccgc acgcgcccgc
      661 ccgaatcgtt ccagaaacgg cgcctccacg aatagcatcg acaggttggc gcaaaatcga
      721 accattcctc cccctctcgg gccagcccat tcggggaagt cccctgctac aatcggccag
      781 ctaacaacac atattctcac cggagtctga caccaaatga ccgagccaca catcctgacc
      841 gccgaggagc ttgcagatcg ccgctatccc tcagacatcg cgatctcggc cgacggccgg
      901 ttcgtcgcgt tcgtcgtcca gccgatgggc aagcgcgagg agcatgatct caccgagatc
      961 tggctcgcgc gcgacggcga gccggcgcgc cagttcacct ccgggctggc cggcgacagc
     1021 tctccggtat tctcatccga ttcggccaaa ctcgctttcc tctccgaccg acatgagcgc
     1081 gagaagcaac gggtttatgt catgcctctc gatggcggcg aggcggtgcg ggtctcgacc
     1141 gtcgaaggca agctctcggc gctgacctgg tcgccggatg gagcgcacct cgccgtgctt
     1201 gttaccgacc ccgaaacgga gacggaaaag aagcagaaag aggagcggaa cgacccgatc
     1261 ctcgagaacc agaaccgcaa gtacgcccgc ctctggtcga tcaacgccga taccggcgcc
     1321 gctcgccagc tcacgttcgg cgagcgacac gtactcgaat acggctggtc ccgcgacggc
     1381 cggacgcttg ccgtggcaac gtcgactcga accgacgtcg aggccgagtt ccaggagatc
     1441 gagatctcat tggttgccgt gttgggcggt cggttgcgcc tgcttgcgac cgtccggggc
     1501 cttcccagca acctgatctt cgtcgaccac tctgatggcg agcagatcgc catgcggcat
     1561 aacggtttca ctgatgaccc ggtcgattcc gtctggctga tgtcgaccgc gaccggcgag
     1621 caccgggaat tgctgaccga cgaccacggc aacgtcgacg caatcatgcc catccccggc
     1681 cgccccggcc agatcggcat gcgcatggtc gatggcgtgc atgccaatgc ctgggtggtc
     1741 gatgtcgcga ccggcgagcg gacgaaggcg ctcgccgccg gctggcagga ccgaggcacc
     1801 ctgcacgccg gaccggcata ctcggccgac ggcgcgaaga tcgcgttcgt ctggaccgac
     1861 tcggtcacgc ccgaggaggc gttcgtcgca gagtccggcg cggagccggt caagctgacc
     1921 tgcttcggca attcgttcgc cggccgcctc aatcgcagcg aactggtcga gtggcagtca
     1981 gatgaatgga cgatccaggg ccatctcgtc tacccgcacg gctacgagga gggcaagcgt
     2041 tacccgctcc tgatcgaggc gcacggcggg ccgtcgtggc agtgggagga tcactgcttc
     2101 ctcgattggc acgactgggc gcagttcatg gcatcccgtg gcttcgcggt gcttgcgccg
     2161 aacccacgcg gctcgaccgg ccggggggag gccttccaga aggcgctcca gaacgacgtc
     2221 ggcggcggcg aagtccggga cctgatcacc ggcgcgcagg cgatggtcga gcgcggcatc
     2281 gccgatccgg atcgacttgg catcggcggc tggagctggg gcggatatct taccgccacc
     2341 acgatcacgc gcacgaacat gttcaaagcc gccatgatgg gcgccgggct ctctaacctc
     2401 ttctcggatc acggcaccga cgacatccca tcggcgaacc tgctctactt cccgggtgag
     2461 ccctaccacc acgccgacat gtactgggaa ggctcggccc tccgctgcgt ttcgcattgc
     2521 acgacgccga ccttgatcct ccatggcgac gccgacgagc gggtccatcc tcttcagggc
     2581 agggagatgt acacggcgct ctcgcggctg gggatcgata ccgagttcgt ccggtatccg
     2641 cggcagggac acacgatcaa ggagcgcaac taccagatcg acctgctcaa ccgcctcgcc
     2701 gcctggttcg agaagtacct gtagtcgtaa ggcagcgaca gcaagatacc gtaatttcgt
     2761 cgtagcgcgg ggatgccctc tttccttgag gagagagtcg gagagaggac cacgccccgc
     2821 ccggacccga tgaaggcccc cgcggtgggg gaatggatcc cgagaatccg aacttgcaac
     2881 ataaaaggcg cgtggggaag catcaagcgg ggcgtggtaa gcccgcgcta cgtcttaatc
     2941 cacgctcaaa aggcgctcga tcgtgagggc gtagatcttg gcttgtgcga cgaggtcgtc
     3001 gatgtagacg gcctcgttgg cgccgtgggc gttctctcca cccggcccca tcccgacggt
     3061 gtcgattccg gcgagacggt aataacgacc atcggtggcg ccggcccact gcacgaagaa
     3121 ctccggctcc tcgccccgca cgtcacgagt cgcgtcccag accgcatgga ccagtggcga
     3181 atccggctcc gtgatgttgg ccgggatgaa atctccgccc gggtcaaccg tcagcgagaa
     3241 ccggaactct ccgcgctccg gcgccgccac gttcatcagg gtttcggtga tctcgtccac
     3301 gaccgactcc cgcgtctcgc cgggaatgag ccgacgatca atcaggaact cacattcgcc
     3361 gggcaccacg ttgtcggcca cgccgccgga tatccggttg atgctgatca ctggtttgcc
     3421 aaccgaggga tgatgccggt cgatgtcccg ttcaagcgcc aggatcgccc tggccgccaa
     3481 tgcgatcgcg ttgacgccgg tcgttcgcgc cgccgcggtg tgcgacgcgc gtccaaggac
     3541 cttcacccgg atgtggagaa tcccgcgctc ggcgttgcgg accttgaggt cgctcttctc
     3601 gccgatcagg caccaagtcg gtttgggcaa ggttccgtct gcgatcgcgt ccatgaccgc
     3661 gacggtcccg tagtgcccgc tcatctcctc gtcgctgacg atgtgcgcct ggaggcagcc
     3721 gcgcatgggt cggtctctga acagctccat catggccagc atgacgccga ccgacgcctt
     3781 catgtcggac gagcctcggc caaagagccg gacgccatcc tccgatatca cagggtcgaa
     3841 cgggccggtt ttccaggcgt cggcatcgga aacaggaacg acgtcgaggt ggccattcat
     3901 ggccagcgtc ggcccggcag atgggtcgcc gatgctgatg atcagattgg gcctgcgctc
     3961 atcgcgctcg atgacggtca cgtcgaggtt gcgctcgcgg caccaggtca tgagccactc
     4021 catgatcgcg gcttcatcgc ccgagacgct gggaatggca atcaatgacc gggcaatcgg
     4081 aagcaccgtg tcccgatcga ttcggtccac tatcgaagtc atgaattctc ctggagttgg
     4141 gctagagtcg ctcgaaccgg ttgacgttca tcacgaaaac aatcgcgccg ccaacctcga
     4201 cttcgatgtt ttcggggagg tagagcaggc ctggctcgag ggccggggaa tagggaacgg
     4261 caacctgggt gcggcgccgg caggtccgtt cgaggatgcg catgatcgag tcgacgtact
     4321 ggtcctcgac gccgatcagg agactggtat tgccggtgcg caggaatgag cccgtcgagc
     4381 tgaccttcgt gaaccgatga cccgcgctaa tcagttcctc ggtcagagca tcaacatcct
     4441 cgtcctgcac aatggcaatg acaagtttca ttctccccgt tcctccgctc tccgccgtcc
     4501 accaatggct cttgtctgtc catgcaggat cggctgagtc tagcagatga tcgagttgac
     4561 gctcagacta caggcgtcgc cggttttcct tgcccaagcg gatcaatccc cgctccagca
     4621 acgccgctga ggtcgagagc acctggctga tcggcatact ggcctgctcg gccacggccc
     4681 gcagtggccg gtcgccattg accccgttca gtacagacca ctcggcgggc gatacctcga
     4741 acgtctcgtc gacttcatac ggctggcgaa gcacgaggtc gacgatcgcg tcggcgctca
     4801 gctccagatc gcccgacccg ggccgcgcct gcacctcgtc gacctgcttg ttctggtcat
     4861 cgagccgctg ggcgatcgtc gaggcggtct cctcgagctg agcggagtaa tgctcgagat
     4921 cgccggtctg gttgacgtcg acggcctcga tcaccgcaac cgcacggcca tcggccagca
     4981 ccggcacgcc gagatatgag cgcaaaccaa gatcggcaag gctcggaccg gtctcgggcc
     5041 gccgatcccg cgagtcgtag aggaccggtc ggcgttgctc gaacgacacg cgggaccaac
     5101 cgagtgtctc cgaacaggcc agaccgtaat agaaggcgcc atcctcgagc actgtcgcca
     5161 catgccagcg acggccgcgc gcgaccggcc ggaagaacga gacccggcga gcgccggtcg
     5221 cgttctgggc gtgctggatg accatggtca cctggcggtc ggtatccggg acgacggtcg
     5281 tcccgccgag ctcggcggtg atgtgtgcct gggcacgccg ctggagctgg gtcgtgtaac
     5341 gctttcgctc atcacgcatc ggctcttcgg agtaggagaa cggctcctcg tgcccgttga
     5401 gcgtcggcgg ggcggagtca attggcggca tgcgcaatga tcactttcgt cgcttgccgg
     5461 aacgcgcgtt ggttacccga tcgtgaggag cgttacggtg ctcaccacgc cgggcgtctt
     5521 ctgtatcgtg ccaatcacga tctcgccgat cttcctgggg tccgttgcgt cgatcaccgc
     5581 caccaggtcg tagattccgg caagttggtc aacctgcccg attcccgctt cctgcagcgc
     5641 tttctgaatc tcggaaccct tgcctgcctc gatcgtgatc aacacatacg cgcgcaccgc
     5701 ttctcttccc tggccctgga ccagccccga acggggttac tccctggctc gctcccataa
     5761 tgtcttcatg ccgacaccgc gcaccatatc gcaaaggtac tgtcgttcca atttcaggag
     5821 gaagcgataa ctgtcctgcg cctccgcgac cagggccttg ccgccaacgg catcgcaccg
     5881 ctcggccagg atcgtgtaca tgccaatgtc gcggaattcc cgcacgccgg gggtccagaa
     5941 ggcgagcgtc aactgctcag gcaggggaag atgcggccgg agcctgccga acgagaggaa
     6001 acgatcgtgc gcagaggcga cgatctcgtc ggtcatgacg cgcgggccaa cgttctcggt
     6061 ttgccgaaaa tcgatgatca gcgattgcga caccctggtg aagaggatgc tcatcacctc
     6121 ggctgtttgg agcagctcgc gtagccgcgc ctcgaaatcc tcagcctcat cgtgagattg
     6181 cggatcatcc atggtctaca ccaccggcgt tcccacattg ctgattgccg ccccgatctc
     6241 gtcgatgagc tcggcgtgaa tcaacccatt gcttgccgcc aactcctgtc caaacacatc
     6301 gtacgggctt ccgtcaattg cgctcactgc tccgccggcc gcctgcacaa tgatcgagcc
     6361 agcgcccaca tcccaggcgt tgatgggtcg ctcataaaag ccgtcggtcc ggccggcggc
     6421 gacccaggcc actccgagcg cggccgagcc atcccgccgg atgccctggc acttcccctg
     6481 cagatgccgc cagatctcat gctgctcgac ctgttgttgt tgctcatatg aaaatcccgt
     6541 accaatcaac gatcgagaca tgacatcaac agtcgagacc tggatcggtc gaccgttgag
     6601 tgtcgcccgt ccgccccgtc gcgcggcgaa gagctcgtcg cgggacggat caaagactac
     6661 gccgatctca ccgaccccct ggtgcacgac ggcgatcgag acag
//
LOCUS       DATAOZ010000243         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3784570, whole genome shotgun sequence.
ACCESSION   DATAOZ010000243 DATAOZ010000000
VERSION     DATAOZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3784570"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="VHR64_06475"
     CDS             <1..1598
                     /locus_tag="VHR64_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3302511.1"
                     /translation="FDLLEPDWDSFGTALREANHRFPGFDQIGFDRFVNGPESFTPDS
                     HAIVGPVVGWDQLYVCAGFNSFGIAGAGGMGKAIAEWIIAGEEPMDLWEVDSRRFAPY
                     QNEPNYLKARVSEVLGHHYELAWPTTEFHSARNLKQSPIHDRLAASGACFGQRIGWER
                     PLWFAGPGKTPVFDQSWGRASWFDNWAREHRATREAVALFDQSSFGKLRVTGPDALRL
                     LDRVSANNVNVAPGRLVYTAWLNRHGRFVSDLTVVRHGKNEFSVMTAAIQPVHDRDWL
                     ERQAWVISADVCIEDVTDQTAVIGVMGPNSRALLGRVTDAGISNAAFPFAAVRSISVA
                     GTQLIAQRVTYVGELGWELHIPAADAVTVFDALVEAGQDLGVTLAGTMAMNSLRMEKG
                     YVSWSHDVSRDDTPLEAGLGFALDLTLLGGIHGRRALLKQRESGIRRCLVTFLLEDSE
                     PLLWGHEPILRDGTPIGFTTSGSYAHTLGAAIGMGYVRSDEPITREWVEAGRYEIETA
                     GTRFAARPHWRAPYDPSRSRILA"
     gene            1656..2597
                     /locus_tag="VHR64_06480"
     CDS             1656..2597
                     /locus_tag="VHR64_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3302512.1"
                     /translation="MARIQDRIGFGFRPSDATSAIDKIVRAEEAGFSTAWTVMPALNR
                     DTPTILAAAAMRTGVIKLGTSIVPAFTRHPLALATQALAIEDLAPGRLRLGIGTSHQR
                     TMIAAYGFEFNKPLTQLREYLTVLRTVLHDGKVDTEGEFYTAHAAFQNPPGTPVLISA
                     LREHAFELAGELGDGGISWITPASYLTSVSKRAMERGAQKANRPVPPLIAHVFVSARE
                     NRDQVRSAVRASLKYYAEAPFYQKMFAASGFPLGENNAIPDGLLDAITISGSRNGIAE
                     GLQSRLDSGIDELLIDIVPGDDQIAEENAVFEVVKSL"
     gene            complement(2798..3175)
                     /locus_tag="VHR64_06485"
     CDS             complement(2798..3175)
                     /locus_tag="VHR64_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302513.1"
                     /translation="MKRLAKFGIGLVAGGAVGAAVGTLTAPEDGESFRSRMRRHVSEA
                     RRAGDEARATKQAELISQYRKDVGDHDALEEEVDHTLSTTDAVLAMGLGLNAPGAIAS
                     QQAALRDSDVLEGADSGAAKARS"
     gene            complement(3198..>3731)
                     /locus_tag="VHR64_06490"
     CDS             complement(3198..>3731)
                     /locus_tag="VHR64_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302514.1"
                     /translation="RDWTADMAETNPTDNGLNPVMQPSDATGEFVEAEELLHGDAGDV
                     TAARVTLEESTAKSITGDHVTMTQSAVKNIEAQTATMTQSAAMSVKSADIALHQSSTG
                     IVSGERVDIFDSMVGVVRGPVTVGEGASARVFVQVGPGNASVRPMLSAQAALGLGAGF
                     GATLVLLSRLLRRLLGN"
BASE COUNT          630 a         1240 c         1181 g          680 t
ORIGIN      
        1 ccttcgactt gctggagccg gactgggatt cgttcggcac ggccctgcgc gaggccaatc
       61 atcgcttccc cggcttcgat cagatcggct tcgaccgctt cgtcaacggt ccggagagct
      121 tcacgcccga cagccacgcg atcgtcgggc cggtcgtcgg ctgggatcag ctctacgtct
      181 gcgccggatt caactcgttc ggcatcgccg gtgcgggcgg tatgggcaag gcgatcgccg
      241 agtggatcat cgccggcgag gagccgatgg acctctggga ggtcgacagt cgccgcttcg
      301 ccccatacca gaatgaaccg aactacctga aggcgcgcgt ctcggaggta ctcggacacc
      361 attacgagct ggcatggccg accactgagt tccactccgc gcgcaacctg aagcaatcgc
      421 cgatccacga tcgtctcgcg gcctccggag cctgtttcgg gcaacggata ggctgggaac
      481 ggccgctctg gttcgccggc ccgggcaaga cgccggtctt cgaccagtcg tggggccgcg
      541 cttcctggtt cgacaactgg gcccgcgagc accgggcgac gcgggaggcg gtcgcgctct
      601 tcgaccagtc ctcctttggc aagctgcgcg tgaccggtcc ggacgcgctc agattgcttg
      661 accgggtctc ggcgaacaac gtgaatgtcg cgcccggccg cctcgtatac accgcctggc
      721 taaatcgcca cggccgcttc gtcagcgacc tgacggtcgt gcggcatggt aagaatgagt
      781 tctcggtcat gaccgctgcc attcagccag tacacgaccg cgattggctt gagcggcagg
      841 cgtgggtgat ctcggccgac gtctgcatcg aggatgtcac ggaccaaacg gccgtaatcg
      901 gcgtaatggg accgaattcg cgcgctttgt tgggtcgcgt gactgatgct ggtatttcga
      961 acgcggcatt cccattcgcg gccgtgcggt cgatttcggt tgccggcacc cagttgatcg
     1021 cccagcgggt gacctacgtc ggcgagctgg gctgggagct gcacatcccg gcggccgacg
     1081 cggtgacggt cttcgatgcg ttggttgagg caggtcagga tctcggcgtg acgttggccg
     1141 gcaccatggc gatgaattcg ctgcggatgg agaagggcta cgtctcatgg agccacgatg
     1201 tctcccggga cgacacgccg ctcgaagcag ggctcggatt tgctcttgac ttgactctgc
     1261 tcggaggtat ccacggccgt cgcgccttgc tcaagcagcg ggaatcagga attcggcgct
     1321 gcctggtgac gtttctgctt gaggactcgg aaccgctgct ctggggccac gagccgatcc
     1381 tgcgcgacgg cacaccgatc ggcttcacga cctccggcag ctacgcgcac acgcttggtg
     1441 cggccatcgg catgggctat gtgcgttcgg acgagccgat cacgcgcgag tgggtcgaag
     1501 ccggtcggta cgagatcgag accgccggca cccgttttgc cgccaggccg cactggcgcg
     1561 cgccatacga cccctcacga tcccgaatcc ttgcgtaagg cagactaaaa gcgtcgcatg
     1621 gcaatgcgac cttcacgcat caccggaggt acatcgtggc acgcattcag gatcgtatcg
     1681 gattcggctt tcgcccgtca gacgccacgt cggcgatcga caagatcgtc cgggccgagg
     1741 aggctggttt ctcgactgcc tggacagtga tgccggcgct gaaccgggac acgccgacga
     1801 tcctggcagc ggccgcgatg cggactggcg tgatcaagct cggtacgtcg atcgtgccgg
     1861 ccttcacgcg ccatccgctt gcccttgcca cgcaagccct ggcgatcgag gacctcgcgc
     1921 ccggccggtt gcgactcgga atcggcacca gccaccagcg gacgatgatc gccgcctacg
     1981 gcttcgagtt caacaagcca ctcacccagc tccgcgaata tctgaccgtg ttgcgtactg
     2041 tgctccatga tggcaaggtc gacacagagg gcgagttcta cacagcacac gccgccttcc
     2101 agaatccgcc cggcacgccg gtgctgatct cggcgctgcg cgagcacgcc ttcgagctgg
     2161 ccggcgagct aggcgacggg ggcatcagct ggatcacgcc ggcgagctac ctgaccagcg
     2221 tctcgaaacg ggccatggag cggggagcgc agaaggccaa ccggccggtt ccgcccctga
     2281 tcgcccacgt cttcgtctca gcgcgcgaaa accgcgatca ggtccggagc gccgttcggg
     2341 cgtcgctcaa gtactacgcc gaggcgccgt tctaccagaa gatgttcgcc gcctccggct
     2401 tcccgctcgg cgagaacaac gccatcccgg acggcctcct cgatgcgatc accatcagcg
     2461 gcagccgcaa cggaatcgcc gagggcctgc aatcccgcct cgactcaggc atcgacgagc
     2521 tcctgatcga tatcgtgcca ggcgacgatc agatcgcgga agagaatgcc gtcttcgagg
     2581 tggtcaagtc actctgaggc gcggagtcgg gaggcgcgaa gttggagata aagtgcagtg
     2641 gcggaggaac gctttctccg ccactactca cgtcgccgat tgaccccata gagggcgtgt
     2701 cagacgaagt attcgttatc tgctgaagtg ccgggcggct cgcccggcac taggttctct
     2761 tggcacatcg ttcggtcacc actaagcatt atcaagctca gcttcgcgct ttggcggctc
     2821 cggagtccgc gccttccagc acgtcgctgt cgcgcagggc cgcttgctgc gaagcgattg
     2881 cgcccggggc gttcaagccg agccccatcg ccaggacggc gtcggtcgtt gagagggtgt
     2941 ggtcgacctc ttcctcgagc gcatcatggt cgccgacgtc cttgcggtac tgggagatca
     3001 actcggcctg cttggtggcc ctggcctcgt caccggcccg gcgcgcttca gacacgtgcc
     3061 gccgcatccg gctccggaat gattccccat cctcgggagc ggtcagcgtg ccgaccgccg
     3121 cgccgaccgc gccgccggcc accaggccaa ttccgaactt cgcaagccgt ttcatcagtg
     3181 ccatctatgt accgctttca gttgccgagc agccgccgca gcagccgtga cagcaaaacc
     3241 agtgtcgccc caaaccctgc gccaaggccc aatgccgcct gcgcgctcaa catcggccga
     3301 accgaggcgt tgcccggtcc gacctggaca aatacccgtg ccgacgcgcc ctcaccgacc
     3361 gtgaccggac cgcgcacgac cccgaccatc gagtcgaaga tgtcgacccg ctcgcccgaa
     3421 acgatgccgg tgctgctctg gtgcaacgcg atatcggccg atttgacgct catcgcggcc
     3481 gattgggtca tggtcgcagt ctgggcttcg atgttcttga ccgcactctg cgtcatggtc
     3541 acgtgatcgc cggtgatcga cttcgcggtc gattcttcga gcgtgacccg ggccgccgtg
     3601 acatcgccgg catcgccatg aaggagttct tccgcctcga cgaattcgcc tgtggcgtcc
     3661 gatggctgca tgaccgggtt cagaccgttg tcggtcgggt tcgtttctgc catgtctgcg
     3721 gtccaatcac g
//
LOCUS       DATAOZ010000244         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_365160, whole genome shotgun sequence.
ACCESSION   DATAOZ010000244 DATAOZ010000000
VERSION     DATAOZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365160"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VHR64_06495"
     CDS             complement(<1..422)
                     /locus_tag="VHR64_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX3302515.1"
                     /translation="MASATAVAEPDSAPQKSFIDAIHDTLAEEMRADDRIVILGEDVG
                     RRGGVFRVTAGLLDEFGSERVIDTPLAESCIIGSAIGMALNGLRPIAEIQFLDFIHSA
                     MDQIMSEAARIRYRSNNDFSCPLVIRVPFGGGVHGALY"
     gene            complement(486..1622)
                     /locus_tag="VHR64_06500"
     CDS             complement(486..1622)
                     /locus_tag="VHR64_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX3302516.1"
                     /translation="MTTDVTNAPDKAVQGAFNPGLTTTTEHHPGHLPPSSATFEATTT
                     SARVAPSSTGDAGLSAEILIDMYRHLALARALDERMWQLNRSGRAAFIVSGQGHEGAQ
                     VGAGFALKRGTDFLVPYYRDLALSLVFGLSATDVMMAALARAADPTSGGRQMPAHYGS
                     ARLNIVTSSSPVATQIPHAVGIAYAAKMRKTGAVAITCFGEGGSSKGDFHESCNFAAI
                     HKLPVIFFCENNNYAISVPIEEQVSVPHVADRAVAYGFPGVVVDGTDVLAVYQVIKEA
                     ADRARNGEGPTLVEAKVYRFQPHTSDDDDRAYRDPDEVKAWRARDPLVLFEARLRELG
                     VLDDDQIARIRSGIGQEVDHATEAAENAPLPDAATFDRHVYADR"
BASE COUNT          388 a          653 c          644 g          377 t
ORIGIN      
        1 tgatagagcg caccgtggac tccaccgccg aacgggacgc ggatcacgag cggacaggag
       61 aagtcgttgt tcgagcggta ccgaatcctg gccgcttcgc tcataatctg gtccatcgca
      121 gagtggatga aatcgaggaa ctgaatttcg gcgatcggcc gtaacccgtt caaagccatg
      181 ccgatcgcgc tgccgatgat gcaggactcc gccagcggcg tgtcgatcac tcgctctgac
      241 ccaaactcat cgagcagacc ggctgtcacc cggaacacac caccgcggcg accgacatcc
      301 tcgccgagga tcacgatccg gtcatcagcg cgcatttcct cggcgagcgt gtcatgaatc
      361 gcgtcgatga acgacttctg cggcgcggag tctggctcgg cgaccgctgt tgcagaggcc
      421 atcgttacct gatcctgaac ctgggccatg gtgcttcctc acggtgtgcg cgtcggctat
      481 gccgttcagc ggtccgcata gacgtggcgg tcgaacgttg ccgcatcggg gagtggagca
      541 ttttcggcgg cttcggtcgc gtgatcgacc tcctgaccga tcccggaccg tatccgcgca
      601 atctggtcat cgtcgagcac gcccagctca cgcagacggg cttcgaacaa gaccagcgga
      661 tcgcgagcac gccatgcctt cacctcatcc gggtcccggt aggctcgatc gtcgtcatcc
      721 gaggtatgcg gttggaaccg gtagaccttt gcctcgacca gcgtcggccc ctcgccattc
      781 cgcgcccggt cggctgcttc cttgatgacc tgatagacgg ccaggacgtc ggtgccatcg
      841 accaccacgc ctgggaagcc ataggccact gcccggtcgg cgacatgcgg gactgaaacc
      901 tgctcctcga tcggaaccga gatggcgtag ttgttgttct cgcagaagaa aatcaccggc
      961 agcttgtgga tcgcggcgaa gttgcaggat tcgtggaagt cgcctttcga agaaccgcct
     1021 tcaccgaagc aagtgatcgc gaccgctccg gttttccgca tcttggccgc atacgcgatt
     1081 ccgaccgcgt gcggaatctg tgttgccaca gggctcgagc tggtgacgat attgagccgg
     1141 gccgagccgt aatgagccgg catctgtcgg ccaccggatg tcgggtctgc cgctcgggcg
     1201 agcgccgcca tcatcacgtc ggtcgcgctc aggccgaaga cgagactgag cgcgagatct
     1261 cgatagtacg ggacgagaaa atcggttcct cgcttgagcg cgaatccggc gcccacctgc
     1321 gcgccctcgt gcccctgccc ggagacgatg aaggcggcgc gacctgagcg gttgagctgc
     1381 cacatccgct cgtccagggc acgcgcgagg gccaggtgcc ggtacatatc aatcaggatt
     1441 tcggcggata gaccggcgtc accagtggat gacggagcga ctcgtgccga agtcgtggtg
     1501 gcctcaaagg tcgcggaaga aggggggaga tgaccggggt gatgttcagt cgtcgtggtc
     1561 aaaccggggt tgaacgcgcc ctggaccgcc ttgtccggag cattggttac atcggtcgtc
     1621 attgaccgca cctcacttac tcgagaccag gtctcttgag ccgccaaagc cggcggccga
     1681 gtgatgtctt gattcgatca tagcgcagtc ggacaagccg tccagtcgcg ctcggcaccc
     1741 gatcggatgg gaggtcggtg ggaccaaagt accagcccgg ctcgggtaat ccctcgcatc
     1801 gtgatccatt gggcaaggtc tatactcgat ttcaggtggt cgcaacgacg cgactgccgg
     1861 gtccacgtca ggagaccggg taccacctat gtccatgcct ctgaccttcg agctcaccga
     1921 cgagcagcgc gccctccgcg acaccatccg cgaattcgca gcggccgaaa ttgcacccca
     1981 cgccgcggaa tgggacctca accacacctt cccgaccgag actgtccgca agctgggcga
     2041 gcttggcgcg atgggagttg cc
//
LOCUS       DATAOZ010000245         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_889027, whole genome shotgun sequence.
ACCESSION   DATAOZ010000245 DATAOZ010000000
VERSION     DATAOZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889027"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1051)
                     /locus_tag="VHR64_06505"
     CDS             complement(155..1051)
                     /locus_tag="VHR64_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX3302517.1"
                     /translation="MSEKPAAGSQLRRGGLGRGLASLIPATGAPAASETSSVAIDAIR
                     PNPYQPRTEWDEERLQELSESIRTHGLIQPLIVSVGREPETYVLIAGERRLRAARLAG
                     LSTVPVVVKDVAPQALLELALVENLVRADLSPLEEAQAYRQLIEDFGLTQAAVAERVG
                     RSRVSVTNTLRLLNAPDEIKAALAGGQITEGHARALLALPTAIDQISALEDVIAKGLS
                     VRQTEDLVRKWSTKHEPTTRHPRSDDRLSWQHADRLQRALQTKVAVKRDASTGQGSIT
                     IHFFDDEQLSSLLERLAGEEYF"
     gene            1207..2133
                     /locus_tag="VHR64_06510"
     CDS             1207..2133
                     /locus_tag="VHR64_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX3302518.1"
                     /translation="MLIISIIFLFLVGFAYFLVWWAHEAEKDRSAMVGLYLILGAPGF
                     LLFIAGLAVTTRRGVGGLGPTLLLGGLGLMLPLFRPIRVQLSKIGDFDPDDPVHMLGT
                     GIFLGAIGFIGGQLISAPEPEEVSSFDVGPIIAQGVFFVLLAVVAVGYHVDRTWPEVR
                     ERLGLVRPTLPGVGVALGSVVVGFITSGIASELTRATNEKYANEINRSLEVMTTSSPS
                     YIAAPLIGLSAGIGEELLFRGALQPRYGIIPTSILFALLHSQYGFSFITLGTFLLGCI
                     FGVIAKRYGTTHAIIAHTTYNTLAVIISASAR"
BASE COUNT          401 a          716 c          708 g          467 t
ORIGIN      
        1 ggatcgccgt ccaggttgac cccgcgaccg gcggcacgtc ctggcacatc gagtacgagt
       61 tccggggaga ccagccgcag ccttctatga cgatggacgt cgactggcaa acagccgata
      121 tccgaaatgt cgagaacgcg acggagccgt gcccctagaa gtactcttcc ccggccaggc
      181 gctcgagcag gctcgagagc tgctcatcgt cgaagaagtg gatggtgatc gacccctggc
      241 ccgttgacgc atcgcgcttg accgcaacct tggtctgaag cgcgcgctgg agccgatcgg
      301 cgtgctgcca gctcaggcga tcgtcgcttc gagggtgccg cgttgtgggt tcgtgcttcg
      361 tcgaccactt tctgaccaga tcctcggttt ggcggacact caagcccttg gcgatgacgt
      421 cttcgagtgc cgatatctga tcgatcgctg tcggcagcgc tagcagggct cgggcgtgac
      481 cctcggtgat ttgtccgccg gccagcgcgg ccttgatctc gtccggcgcg ttgagcagtc
      541 gaagcgtgtt ggtcacgctg acccgcgacc ggccgacccg ctccgcgacc gccgcctggg
      601 tcaatccgaa atcctcgatc aattgccggt atgcctgcgc ctcttccagc ggggaaagat
      661 cggcccggac caggttctcg acaagagcga gctcgagtaa tgcctggggc gcaacatcct
      721 tgacgaccac cgggaccgtg ctcaacccgg ccaggcgagc ggctcgcagc cgtcgctcgc
      781 cggcaatcag cacgtaggtc tccggctccc gcccgaccga cacgatcagc ggctggatca
      841 acccgtgtgt ccgaatggac tcggacagct cttgcagacg ctcctcgtcc cattcggtgc
      901 gtggctggta gggattgggc cgaatcgcat cgatcgcgac gctgctggtc tctgaggctg
      961 ccggggcgcc ggtggcggga atgagcgacg cgagtcccct gcccagtcca ccgcgacgca
     1021 actggctacc ggcggcgggc ttttcggaca cggagattca cctcgaaata cgcaggatcg
     1081 cgcgtgggaa gtctagcagg gacgccgcac agcccggcgt ggggaagacg agtcgaccct
     1141 atactttcgc cggttcggag cggcgctccg ggcctttgtg ctgcaaacga aggcggacca
     1201 tcgaccgtgt tgattatctc aatcatcttc ctgttcctgg tcgggttcgc atatttcctg
     1261 gtctggtggg cacacgaagc ggagaaggac cgatcggcga tggtcggtct ttatctcatt
     1321 ctcggcgcgc cgggcttcct gcttttcatt gctggacttg cggtaaccac tcgccgcgga
     1381 gtcggcggac ttgggccgac actcctcctg ggcggactcg gattgatgtt gccgctcttc
     1441 cgaccaatcc gggtgcagtt atcgaaaatt ggcgacttcg atccagacga tccggtccac
     1501 atgctcggta ctggcatctt cctgggagca atcgggttta tcggcggtca actcatttca
     1561 gcacccgagc cggaagaggt cagctcgttc gatgtcggcc cgatcatcgc gcaaggtgtc
     1621 ttcttcgtcc tgcttgcggt cgtcgccgtc ggatatcacg tcgaccggac ctggccggaa
     1681 gttcgggaac gacttggctt ggtcaggcca acattgccgg gagtcggggt tgccctcggg
     1741 tcggtggttg ttgggttcat cacctcggga attgcgtcgg agcttacgcg cgcaaccaac
     1801 gagaagtacg cgaatgaaat taaccgatcg ctggaagtga tgacgacgtc cagtccgtcc
     1861 tacattgccg ctccgctgat cggtctgtcg gctgggatag gtgaagaatt actgttccgg
     1921 ggcgccttgc aaccgcgcta cggaatcatt ccgacatcga tcctgttcgc gctgctgcac
     1981 agccagtacg gcttctcgtt catcacgctt ggcacgttcc tgctcggctg catcttcggc
     2041 gtcattgcca agcgctatgg aacgacccac gcgatcatcg cgcataccac ctacaacaca
     2101 ctggccgtga tcatctcggc atcggcccgc tagtacaatc gccgtcacga cgatttcgcg
     2161 gttattggag cgcgggacag ggatgaagct agccgaaatc cgcgacctcg acggtcccaa
     2221 tctgatcctg ctccggcccg ctatcaaggt cgagctcata ctcgaggacg gtgagaccga
     2281 atcgaagctc gc
//
LOCUS       DATAOZ010000246         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8785096, whole genome shotgun sequence.
ACCESSION   DATAOZ010000246 DATAOZ010000000
VERSION     DATAOZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8785096"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VHR64_06515"
     CDS             <1..830
                     /locus_tag="VHR64_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302519.1"
                     /translation="LRKFSRARVARSTAGMTENQPDPTTTTVVNTEQPVEKKSGWGPF
                     GGRNNQPPDHEIERITIRSYPKIIFLYPSVVFAFIAAIFSSADWGSPDNWGLAFMIVL
                     ACNFFVLAFDFPRTASLTLFFSIIAIVLAAILLNERAEFLPALADKTGNLKPTANDTF
                     YWMYAIITALIIGIVWVIHRFIDYYEVLSNEIIHHHGILGGIERLPAPGVRLEKDITD
                     VFEYLLLRSGRLIITPAGESRAIVMDNVPNINHVEDGVKAILGVTEVSFRQRDATLR"
     gene            1025..1534
                     /locus_tag="VHR64_06520"
     CDS             1025..1534
                     /locus_tag="VHR64_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX3302520.1"
                     /translation="MTVVTQPLRDEHNELFPHVDQLRAAADGVGESDPATLEAVREAL
                     DFLEHHLIPHAEAEDRALYPVVARIMGAPDATATMRRDHVEVGKLTTELGMLREELAM
                     AGGSPFLYRELRRVLYGLFALVELHFAKEEEVYLPILDANLTAEQASDLFHKIEAAAG
                     HSQRHVHAG"
     gene            complement(1538..1873)
                     /locus_tag="VHR64_06525"
     CDS             complement(1538..1873)
                     /locus_tag="VHR64_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX3302521.1"
                     /translation="MTDATKNLVYYPVAKVGDIGPGELKYVEAGPDDEPICLINYEGE
                     IFAMGDCCTHEYASLADGEIVGDELECPLHGGAFEIKTGMPANMPVVVPNVMYPVKIE
                     NDTILIGVD"
     gene            complement(1866..>2258)
                     /locus_tag="VHR64_06530"
     CDS             complement(1866..>2258)
                     /locus_tag="VHR64_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX3302522.1"
                     /translation="QRMDSLYKEIVLDHYKNPHNFGELDPADYTAEDSNPLCGDQVRM
                     DLRVEDGIVTDVRFSGRGCAVSMASASILTDMIKGQPLEEVLQITKDDLLEEIGIPVS
                     PARMKCATLGLNVLKLSVALKEAHSHGD"
BASE COUNT          409 a          750 c          666 g          433 t
ORIGIN      
        1 gattgcgcaa gttcagccgg gccagggtgg ccaggagcac agccggaatg acagagaacc
       61 aacccgatcc gaccacgaca acggtcgtca acacggagca accagtcgag aagaaatcgg
      121 gttggggacc cttcggcgga aggaacaacc aaccgcctga tcacgagatc gagcggatca
      181 ccatccggtc gtatcccaag atcatcttcc tttatccgtc ggttgtcttc gccttcattg
      241 ccgccatctt tagttcggcc gactggggca gccccgacaa ctggggcctt gccttcatga
      301 tcgtgctcgc ctgcaacttc ttcgttctgg ccttcgattt cccgcgcacg gcgtcactga
      361 cgctcttctt ctcgatcatc gcgatcgtcc tggcggcgat actcctcaac gagcgggccg
      421 aattcctgcc cgccctcgcc gacaagaccg gcaatctgaa accgaccgcc aacgatacct
      481 tctattggat gtacgcgatc atcacggccc ttatcatcgg catcgtctgg gtcatccatc
      541 gcttcattga ttactacgag gtgctctcga acgagatcat ccaccaccac ggcatcctcg
      601 gcggcatcga gcgattgccg gcgccgggcg tgcggctcga gaaggacatc accgatgtct
      661 tcgaatacct gctgctgcgc tccgggcgat tgatcatcac gccggccggg gaatcgcgcg
      721 cgatcgttat ggacaacgta ccgaacatca accacgtcga ggacggcgtc aaggcgatcc
      781 tgggcgtgac cgaagtcagc ttccgccaac gcgacgccac cctgcgctaa actccgcgcc
      841 acacaatcga tcccctggac gagagcgatc ccggctggcg gatcgctctc gtcgactgtt
      901 gcgattttgt gacgctccgt acgccgatgg cggcactccc gccatgcctg gccgcatacg
      961 ctcgggtcag aggccgccgg cattccggca ggcaatctcg cctacccagc gaaaggagcg
     1021 tccgatgacg gtcgttactc aacccttgcg cgatgagcac aatgagctgt tcccgcacgt
     1081 tgaccaattg cgtgccgccg ccgacggtgt cggcgagagt gatcccgcta cgctcgaggc
     1141 ggtccgggag gcgctcgact tcctcgagca tcacctcatc ccgcacgccg aggcagagga
     1201 ccgtgccctc tacccggtcg tggccaggat catgggcgcg ccggatgcga cggccaccat
     1261 gcgccgggac catgtcgagg tcggcaagct gaccaccgag ctgggcatgc tccgcgaaga
     1321 gttggcgatg gctggtggca gcccgttcct ctatagggag ctgcgccggg ttctctacgg
     1381 cctcttcgcg ctggtcgagc tgcacttcgc caaggaagag gaagtctacc tccctatcct
     1441 cgacgcgaac ctcaccgccg agcaagcatc agatctcttc cacaagattg aagccgccgc
     1501 cgggcactcc caaaggcacg tccacgccgg ctgacgccta atcgacgccg atcaggatgg
     1561 tgtcgttttc gattttgacg gggtacatca cgttggggac gacgaccggc atattggccg
     1621 gcatgcccgt cttgatctcg aaggcgccgc catggagtgg gcactccagc tcgtcgccga
     1681 cgatctcgcc gtccgcgagg gaggcgtact cgtgggtgca gcagtcgccc atcgcgaaga
     1741 tctcgccctc gtagttgatc aggcagatcg gctcgtcatc cgggccggcc tcgacgtact
     1801 tgagctcgcc gggaccgata tccccgacct tcgcgacggg gtaatagacc aggttcttcg
     1861 tcgcgtcagt caccgtggga atgggcctcc ttgagggcaa cgctcagctt gagcacgttc
     1921 aggcccaggg tcgcgcactt catccgcgcc gggctgaccg ggatgccgat ctcttccagc
     1981 aggtcgtcct tcgtgatctg caggacctcc tcgagcggct ggcccttgat catgtcggtc
     2041 aggatcgagg ccgatgccat gctgaccgcg cagccgcgac ccgagaacct gacgtcggtc
     2101 acgatgccgt cctcgacccg caggtccatc cggacctggt ccccgcacag ggggttgctg
     2161 tcctcggcgg tgtaatcggc cgggtcaagc tccccgaagt tatgcgggtt cttgtagtgg
     2221 tcgaggacga tctccttgta gaggctatcc attcgttg
//
LOCUS       DATAOZ010000247         7749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1947249, whole genome shotgun sequence.
ACCESSION   DATAOZ010000247 DATAOZ010000000
VERSION     DATAOZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947249"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VHR64_06535"
     CDS             <1..772
                     /locus_tag="VHR64_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302523.1"
                     /translation="LALGACGGDDTSDATATSIQPTASLAIEPTTSGTLAPGEVTAGQ
                     IADRIVAAWPKVTSYRSITRIIPASGTPEATPAVELAGRAERHVILPDQKQIVITDQG
                     STTEIVLVNGVLSKRTTPPGGSPSPWQIIDPATVSPNDPFSRTYETILAPETPPYSGL
                     SGRQRDRIGTEQGQTTIDGRACTGYLFPEVTEIGERIQVIIYLDNADLPCRIETRAGL
                     TISQTDYVFNQETTIATPIAATPAASPVASPAASPTNR"
     gene            complement(769..2136)
                     /locus_tag="VHR64_06540"
     CDS             complement(769..2136)
                     /locus_tag="VHR64_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302524.1"
                     /translation="MRLPKISPAKRPALKLSPAMRRKLAMAMPVAFAALVGLMSVFAS
                     VHDRFNWVPVSHENIVVDSVLKLALAVILLAVAVLITTPFAMAIHVAGHAVAGHIFGM
                     RLLALRIGPLLVTPWAIGNRFEWLSIKSKMDLVTGWVQFDDSSLPNWKRARGWQVMIG
                     GGSAMNLAVSFLCAMISIFASDVTYVLLRQAVWINLLVAVINIVPFVWSRFEFESDGK
                     KLMTLLFNEGDGDEVLMERLRNEVIVGPVRPASWPREREAAWETKLRQTPATGQGKAD
                     QLEMMVYLFVQGVDRSDSEVAWRWVQAMQNALSSDPDNHEIAFETARVMCALYAARWE
                     RNPESAVQFMEQISPESGITANPWYTVARAATSYAESTVATFSAEEKLEEARRRAARA
                     VSQLVEPARLHGIDQMMRGIAQAILGDAEIELQKRDYLARDTGYSEPAELESGLDSED
                     DTEAA"
     gene            2388..3251
                     /locus_tag="VHR64_06545"
     CDS             2388..3251
                     /locus_tag="VHR64_06545"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3302525.1"
                     /translation="MPITNAAETEWVATDLDQTVSQELHGRTERPLSVRVFLWRLLAV
                     GLNIGLFMVLFQLYKLVRKTAITRGETVGFQHAVDIINLEKRLHLFFEPDLQRWILDR
                     PEWVIRGLNYYYVAFMPLFYACCAIGLLFGPVRFRFWRRVFLCSMLLALPWYLLYPLA
                     PPRFMNSDFGYPYGFIDTLKVWGPNYFSSSGLVSANQFAAMPSMHIGWSTIGALTVAT
                     CLPRIKGFPIGWVIGGFHIGMMMLTVMATGNHYFLDEVGGWLVVSAALLLAWFLTDRF
                     PFKLPRWFSPT"
     gene            3357..3998
                     /locus_tag="VHR64_06550"
     CDS             3357..3998
                     /locus_tag="VHR64_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX3302526.1"
                     /translation="MLDADRLLPYTLAVVALILAPGPNQAMVVARSLNGGRRAGIMTS
                     LGVNTGTAFHTVSAALGLSALLATSALAFATVKLLGAAYLLYLGIRLLAGRDHAMGPP
                     QVDAAPAQTIRGANAYGRAIITGILNPKVALFFLAFLPQFVDRLAGSIFLQFLLLGAI
                     IAVVGLAFDSALATAAGSLGRFLARNPRAARWRERITGTAFVALGIRLAFERR"
     gene            4027..4605
                     /locus_tag="VHR64_06555"
     CDS             4027..4605
                     /locus_tag="VHR64_06555"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX3302527.1"
                     /translation="MNEILTNLFHMTEPYPEKIIRSIAVYVFVYIALRVAGKRELGHA
                     STADLIILLLISNTVQNAIIGDDTTLIGGLIGATVLFLLNKGIVQWGYHSHRFTRLVE
                     GIPSDLIRDGEVLMHMLSREKLTIQELAAACREQGVADFADVRRAVLETNGTISVIPK
                     VPSDAVTLQLNEMEATLRELLRRSDTELARQS"
     gene            4616..5581
                     /locus_tag="VHR64_06560"
     CDS             4616..5581
                     /locus_tag="VHR64_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3302528.1"
                     /translation="MSRPSIGLMFFRTYPPETLPDYARMIEQSGLDELWVVEDCFFNG
                     GVSAASVALAVTERIQVGIGILPAVVRNAAYAAMDLATLARIFPGRLHVGLGHGVADW
                     IRQVGQVPVSQMAALEEVTVAIRDILQGQTVTMSGKHVHLDKVRLDHPPTVVPPISLG
                     VTGPKSLALSGRIADGTILVELTGPALVCRNREVIAGGADHQLTIFAYWSQDADGVAA
                     RDRLRRMLAERIRNEGLKELDAPGFADHARELLERGGVDLLAREMPDAWLDEITVSGT
                     PEQCLAAIQRLGDASAARVVLVPPSDATIQDLTPWCRDLAAANQT"
     gene            complement(5729..6418)
                     /locus_tag="VHR64_06565"
     CDS             complement(5729..6418)
                     /locus_tag="VHR64_06565"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX3302529.1"
                     /translation="MCGRYVITWQPDEISERFQLRRLPEGMFQTYMNFNAAPTQELPV
                     IVIDESGERILRPMRWGLRARWSKPGDTKAPAPFNARAESVLEKPMFRNLVKSKRCLV
                     PARGYYEWQNRGDHKQPFLIGLPGDPLIAFAGLYDEFVPQGEDEPVASYTILTTDATE
                     FAGQFHNRMPVILEREDEEDWIDPSNTDVSSILPMAHGYEGEMDAYAVSRAVNNVRNN
                     DPTLLEPIEDE"
     gene            complement(6437..7486)
                     /locus_tag="VHR64_06570"
     CDS             complement(6437..7486)
                     /locus_tag="VHR64_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302530.1"
                     /translation="MEPRPQRPSRATGPYISTGTPNPPTSSANPGSGTGYGTAPFDAS
                     SAPPAPPPAIGYDPDYSPGQPLTPLRGPRGANIVGPLLAVAALAIIVAAVAFIISQFR
                     GGTDNPSSQPTAVIAAAASPTAGQQGDVTGNSPTEAAASNQSGQPTSEAGGAEATTTP
                     KVNRPTETGDLSGSDGTDTSGSSSGEKTRARQWLPTTRIVGEGYEQSNNGTRDETEVA
                     GSFPDPADAATKLAEFGWQENVYREYELSGGAETDTTTINVSVHRFDTEGGAKDALSY
                     FAEGGRVGQGLTEVKDAPEFGEGSVVLQGTIDGGNVYVIYIRQNNFVIRIGGFSPTGD
                     PSETTNALAERMMGQ"
BASE COUNT         1248 a         2580 c         2451 g         1470 t
ORIGIN      
        1 gcttgccctg ggcgcgtgcg gcggagacga cacgtctgat gcgaccgcga catcgattca
       61 gccgaccgcg tcacttgcca tcgagccgac aacatcgggg acgctcgcgc ccggcgaggt
      121 cacggccggg caaattgcgg atcggatcgt cgcggcgtgg cccaaggtca ccagctaccg
      181 ctcgatcacg cgcatcatcc cggcgtcagg cacaccggaa gcgacaccgg cggtcgagtt
      241 ggccggccgc gcggagcgac atgtgatcct gcccgatcag aagcagatcg tgatcacgga
      301 tcagggatcg acgaccgaga tcgtgctggt caacggcgtg ttgtcgaagc ggacaacacc
      361 accgggcggc tcgccaagtc catggcagat tatcgacccg gccaccgtct cgcccaacga
      421 ccccttcagc cggacttacg agacgatcct ggcgccagag acaccgccct acagcggcct
      481 gagcggacgc cagcgcgacc ggatcggcac cgagcagggt cagaccacga tcgacggccg
      541 cgcctgcacc ggctacctct tcccggaggt gaccgagatc ggcgagcgga tccaggtcat
      601 catctacctc gacaacgccg acctgccctg ccggatcgag acccgcgccg gcctcacgat
      661 cagccagacc gactacgtct tcaaccagga aaccacgatc gccacgccga tcgcggcaac
      721 tccggcggcc tccccggtcg cctctcccgc cgccagcccg accaaccgct aggcggcttc
      781 agtgtcgtcc tcggagtcaa ggccggattc gagctcagcc ggctccgagt agccagtatc
      841 gcgggcgagg tagtcgcgct tctggagctc gatttcagcg tcgccgagga ttgcctgggc
      901 gataccgcgc atcatctggt cgattccgtg caaccgcgcc ggctcgacaa gctggctgac
      961 ggcccgggct gctcgccggc gcgcctcctc cagcttctcc tcggccgaaa aggtcgcgac
     1021 ggttgactcg gcgtagctcg tggccgcccg ggcgaccgtg taccacgggt tggccgtgat
     1081 cccggactcg gggctgatct gctccatgaa ctggacggcg gattccggat tgcgctccca
     1141 gcgggccgca tagagggcgc acatcacccg cgccgtctca aaggcgatct cgtgattgtc
     1201 gggatcgctg ctgagcgcgt tctgcatggc ctgcacccat cgccaggcga cttcgctatc
     1261 ggagcggtcg acgccctgaa cgaagaggta gaccatcatc tcgagctgat ccgccttgcc
     1321 ctgcccggtg gccggggtct ggcgcagctt cgtctcccac gccgcttccc gctcccgcgg
     1381 ccatgaggcc ggacggaccg gtccgacgat gacctcgttg cggagccgct ccatcagcac
     1441 ctcatcgcca tccccttcat tgaagaggag ggtcatcagc ttcttgccgt cgctctcgaa
     1501 ctcgaaccgg gaccagacga acgggacgat gttgatcacc gcgaccagca ggttgatcca
     1561 gaccgcctgg cggagcagga cataggtgac gtcgctcgcg aagatcgaga tcatcgcgca
     1621 aaggaacgag accgccaggt tcatggccga gccgccgccg atcataacct gccagccacg
     1681 cgcccgcttc cagttcggta gcgagctgtc gtcgaactgg acccaaccgg tgaccaggtc
     1741 cattttcgac ttgatcgaga gccactcgaa gcggttgcca atcgcccacg gcgtgaccag
     1801 cagcggaccg attcgcagtg cgagcagccg cataccgaag atgtgcccgg ctactgcgtg
     1861 accggccacg tggatcgcca tcgcgaacgg cgtcgtgatc aggaccgcga ccgccagcaa
     1921 gatcacggcc agcgcaagct tgagcacgga gtcgaccacg atgttctcgt ggctgaccgg
     1981 cacccagttg aaccggtcgt gcaccgacgc gaacacgctc atcagtccga ccaatgccgc
     2041 aaacgccacc ggcattgcca tcgccagttt gcgacgcatc gccgggctga gcttgagcgc
     2101 cggccgtttc gccggcgata tctttggaag gcgcatgtcg accccactcc ctggatcggg
     2161 actctccccg tgcggttgga cttgtgatca accgtcaggc aaactttccg ccttgtctac
     2221 cagcggaagg tcccgattcg ggatgaacac cttttcgcac ctcgtgtagc ctgaactgcg
     2281 aacgactttg cccgatcggg caccaatccg gcagaatggc ctgaatcccg gcaacgcctg
     2341 gatccaatac gtacgccgac caaagcggag atatgaacga aagcgcgttg ccgataacga
     2401 acgccgccga gactgagtgg gtcgcgactg accttgatca gacggtcagc caggagctcc
     2461 acggccggac cgagcggccg ctctcggtcc gagtcttcct ctggcgcctc cttgcggtcg
     2521 ggttgaacat cggcctcttc atggtcctgt tccagctcta taagctggtc cgcaagacgg
     2581 cgatcacccg tggcgagacc gtcggattcc agcacgcggt cgatatcatc aacctggaaa
     2641 agcggctgca tctcttcttc gagcccgatc tgcaacgctg gatcctggat cgcccggagt
     2701 gggtcatccg cgggctgaac tactactacg ttgccttcat gccgctgttc tatgcctgct
     2761 gcgcgatcgg cctgctcttc ggcccggtcc gcttccggtt ctggcggcgc gtcttcctct
     2821 gctcgatgct gctggcgctg ccctggtacc tgctctatcc gctcgcgccg ccccgcttca
     2881 tgaacagcga cttcggctac ccctacggct tcatcgacac gctcaaggtc tgggggccga
     2941 actacttctc gagcagcggg ctcgtctcgg cgaaccagtt tgccgccatg ccgagcatgc
     3001 acatcggctg gtccacgatc ggcgcgctga ctgtggccac ctgcttgccg cggatcaagg
     3061 ggttccccat cggctgggtc atcggcggat ttcatatcgg catgatgatg ctgacggtga
     3121 tggcaaccgg caaccattac ttcctggacg aggtgggcgg ctggctcgtc gtctccgcgg
     3181 cgctgctgct cgcctggttc ctgacggacc gcttcccgtt caagctcccc aggtggttca
     3241 gtcccaccta actcagtagc cggtggtcag tggtcagacc agggtaattg acgatccttg
     3301 cttcctccgc gttgaattgc gcaggctcat atcacccaga tcgatggagt tgcgcgatgc
     3361 tcgatgcgga ccggttgctt ccttatacat tggcagtcgt ggcgctgatt ttggctcccg
     3421 gtccgaacca ggctatggtc gtggcccgca gcctcaacgg gggccgccgc gcgggcatca
     3481 tgacctcgct gggcgtgaac accggcacgg cctttcacac cgtttccgcc gccctcggac
     3541 tctcggcgtt gctcgccacc tcggcgcttg cattcgcgac cgtcaagttg ctcggcgcgg
     3601 cctatctgct ctacctcggc attcggctgc tggccggtcg cgaccatgcc atgggccctc
     3661 cccaggttga cgcggcaccg gcccagacca ttcgaggagc caacgcgtac ggtcgcgcca
     3721 tcatcaccgg catcctgaac ccgaaggtcg cgctgttctt cctggccttc ctgccgcaat
     3781 tcgtcgatcg cctggcggga tcgatcttcc tgcagtttct gctgctcggg gcgatcatcg
     3841 cggtcgtcgg gctcgcgttc gattctgccc tggcgaccgc cgccggatcg ctgggacgct
     3901 tcctggcccg caatccgcgt gctgcccgct ggcgggagcg gatcaccgga accgcgttcg
     3961 tcgccctcgg catccgcctc gccttcgagc gccgctagca atcaacacgc accgaggagg
     4021 agccagatga acgagatcct gaccaatctg ttccatatga cggagccgta cccggagaaa
     4081 atcatccggt cgatcgccgt ctatgtgttc gtctatatcg cgttgcgcgt ggccggaaag
     4141 cgcgagctcg gtcacgcgag caccgccgac ttgatcatcc tgctcctgat ctccaatacg
     4201 gtccagaacg cgatcattgg cgacgacacg accctgatcg gcggtttgat cggcgcaaca
     4261 gttcttttct tgctcaataa gggcatcgtc cagtgggggt atcacagcca ccgctttacc
     4321 aggctggtgg agggcatccc atcggatcta atccgcgatg gggaagtgct gatgcacatg
     4381 ctctcgcgcg agaagctgac gatccaggaa ttagccgccg cctgccgaga gcagggcgtg
     4441 gcggactttg ccgacgtccg gcgagcggtc ctcgagacga acgggacgat cagcgtcatt
     4501 ccgaaggtcc cgtcggatgc ggtcacactg caattgaacg agatggaagc cacgctccgc
     4561 gaattattgc gccggtcaga cacggagctg gcccggcaga gttgaggtga atcgcatgtc
     4621 cagaccgtcg atcggcctga tgttcttccg gacctacccg cccgaaaccc tgcccgacta
     4681 cgcccgcatg atcgagcaaa gcggactcga tgagctctgg gtcgtcgagg actgcttctt
     4741 caacggcggc gtctcggcgg cgagcgtggc gttggccgtg accgagcgga tccaggtcgg
     4801 gatcggcatc ctgccggcgg tcgtgcgcaa tgccgcctat gccgcgatgg acctggcgac
     4861 gctcgcgcgc atcttccccg gccggctcca cgtcggactc ggtcatggcg tggcggactg
     4921 gatccggcag gtcgggcagg tgccggtctc gcagatggcg gcgctcgaag aggtcaccgt
     4981 cgccatcaga gacatcctcc agggtcagac agtcaccatg tccggcaagc atgtccacct
     5041 cgacaaggtc cggctcgatc acccgccaac cgtcgtgccg ccgatatccc tcggagtaac
     5101 cgggccgaag tcgctcgcgc tctccggccg gattgccgac gggacgatcc tggtcgagct
     5161 gactggtccg gccctggtgt gccgaaaccg ggaggtcatc gccggcggcg cggatcacca
     5221 actcactatc ttcgcctact ggtcgcagga tgccgatggc gtcgcggccc gggatcgtct
     5281 ccgccgcatg ctggccgagc ggatcaggaa cgaggggctg aaggagctcg atgcgcccgg
     5341 atttgccgat cacgcccggg agctgctgga gcgcggcggg gtcgacctgc tggcccgtga
     5401 gatgccggac gcctggctcg atgagatcac cgtctcgggc acgccggagc agtgcctggc
     5461 cgccatccag cgcctcggcg atgccagcgc cgcccgcgtc gtcctcgtcc ccccgtccga
     5521 cgccaccatc caggacctca ccccatggtg ccgggacctg gcagcagcca accaaacgta
     5581 acgcggcctg cctctgggcc gcttggccgt cgcttttgcc ggtcgtagat gccagatttc
     5641 tccgcccgtc gcgcgggtca tctacggaat gccggccacg gggggccggc gcaacgttga
     5701 cgattggcct cagtttcgcc tattcggctt actcgtcttc gattggttcg aggagggtgg
     5761 ggtcgttgtt gcggacgtta ttgacggcgc ggctgaccgc gtaggcgtcc atttcgcctt
     5821 cgtagccgtg cgccatcggc aggatgctgc tgacgtcggt gttgcttgga tcgatccagt
     5881 cctcttcatc ctcgcgttcg aggatgaccg gcatccggtt gtggaactgg ccggcgaact
     5941 cggtcgcgtc ggtggtcagg atcgtgtacg aggcgaccgg ctcatcctcg ccctgcggga
     6001 cgaactcgtc gtagaggccc gcgaaggcga tcagcggatc gcccggcaac ccgatcagga
     6061 acggctgctt gtggtcgccc cggttctgcc actcgtagta gccacgggcc gggaccaggc
     6121 agcgcttgct cttgaccagg ttccggaaca tcggtttctc gagcaccgat tccgcgcgcg
     6181 cattgaacgg cgccggcgcc ttcgtgtcgc ctggcttcga ccagcgcgcg cgcagtcccc
     6241 accgcatcgg cctgaggatc cgctcgcccg actcgtcgat gacgatcacc ggaagctcct
     6301 gcgtcggcgc ggcgttgaag ttcatatagg tctggaacat accttcgggc aggcgccgga
     6361 gctggaatcg ctccgaaatc tcatccggct gccaggttat gacgtaccgt ccgcacatgg
     6421 cccatgtcct cgcttcctat tgccccatca tccgctcggc cagcgcattg gtggtctccg
     6481 acggatcgcc ggtcggcgag aatccgccga tgcggatcac gaagttgttc tgccggatgt
     6541 agatgacgta gacgttgccg ccgtcgatcg tgccctggag cacgaccgat ccctcgccaa
     6601 attcgggtgc gtccttgact tcagtcaggc cctggccgac tcgaccgccc tcggcgaagt
     6661 acgagagcgc gtccttggcg ccgccttcag tatcgaatcg gtgcacgctg acattgatcg
     6721 tggtcgtgtc ggtctcggct ccgcccgaga gctcgtattc gcggtagacg ttttcctgcc
     6781 agccaaactc ggccagcttg gtcgccgcgt ccgccggatc tgggaatgat ccggccactt
     6841 cggtctcgtc ccgcgtgccg ttgttggatt gctcgtagcc ctcgccgacg atgcgggtcg
     6901 tcggcagcca ttgtcgggcc cgggtcttct ctcctgagga ggagccgctg gtgtcggtgc
     6961 cgtcgctgcc ggagaggtcc ccggtctcgg tcggtcggtt gactttcggc gtggtggtcg
     7021 cctcagcacc tccggcctcc gaagtcggct gaccggactg gttggacgcg gcagcctcag
     7081 ttgggctgtt gccggtgaca tcgccctgct gaccggcggt cgggctggca gcggccgcga
     7141 tgaccgccgt gggctgggat gacgggttgt cggttcctcc gcggaactgc gagatgatga
     7201 aggcgaccgc ggcgacaata attgccaggg cggcgacggc gagcagcggg ccgacgatat
     7261 tggcgccgcg cggaccgcgc agcggcgtca ggggttgacc gggcgaataa tccgggtcgt
     7321 agccgatcgc cggcggcggc gcaggaggcg cgctgctggc atcgaacggc gcggtgccat
     7381 atcccgtgcc actgccggga tttgcgctgc tcgtcggcgg gtttggcgtg ccggtgctga
     7441 tataggggcc cgtggcgcgc gatggtcgtt gtgggcgagg ctccatgaac gagtgcctgt
     7501 tctccggaat tgtgcggttc tgtcaactga cgacgcgccg atcgctgcgc ggtgcggacg
     7561 ctccccgtct gtcgggatcg gtgcgtgccg tatgagtata cgtttcaagg cggtcaatac
     7621 tcggcaaatc ccggtatcga cttcctgtca ctagcgaaaa cgcccggtgc ggccggattg
     7681 tttccccgca ggagaaggtc tttcgttttt cgtcttcggg ttttgggttt tgggttttgg
     7741 gttttgggt
//
LOCUS       DATAOZ010000248         7945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8523883, whole genome shotgun sequence.
ACCESSION   DATAOZ010000248 DATAOZ010000000
VERSION     DATAOZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8523883"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="VHR64_06575"
     CDS             <1..1938
                     /locus_tag="VHR64_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302531.1"
                     /translation="CRRGQCVALCPPDQIYCGDGCYDPLTDNTHCGDCGVDCGGLTCC
                     GGVCIDTNADPANCGQCGSACSANATCESGQCVSPCPAGTTPCGSTCVDFTSDPSNCG
                     NCGNACPADSTCTNATCVPICPAGFTPCNGACVDIGTDPQNCGACGQICGSKACQNGT
                     CLDGPCFVSQDGPELSFRYETESAADNLSLRTTRTVLSDDSGATLSIITRTTVSRDRQ
                     TVIRINHDYDAGGAWTLTLHYGDAFDGINRAVFSGQGDSPITGKVDRRRISPFTPGAD
                     PSTLAFADGKPAPTITVDPALVDALGRIFAQAEQETSACTPVDQTNAILSPLSYGIAA
                     TAPITIYAPDMHAPTPAGAGYCDPGHHSFPPLGSAGCVACRGGCLAAGGVCGYSAAAG
                     CSAGCALALAGYGVCLGICLAAALVACAAGELKCQNECRKPPGACCPSGCSDGCCDNG
                     EICLDQASGLCCGGEFTKPCQGKCCCSRTDNCLPDGTCCPQGNKICNDTCCNSGETCF
                     EGGCCAKVCDKVTPRTCCGAFDTCTECGCCPPGTTCTTDKSDRKICCPDGRLCGDVCC
                     PNGEVCQDPRTGTCFGSTSCPPGQVACPGGTCCPTVKAQGGGTLYTCCGNVCCPSDKP
                     YCCSPGDDTHAAVCNAVGCIR"
     gene            complement(1967..2965)
                     /locus_tag="VHR64_06580"
     CDS             complement(1967..2965)
                     /locus_tag="VHR64_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX3302532.1"
                     /translation="MKPFAYTSAESLAEAIAILSQESGNGTRPLAGGTDLLTLMKPEI
                     ATPSHLVDIKRVSDLSDRIDESEDGVTIGTLCTLAEIERSELLRTRYMALAQAAELAA
                     TPQLRNMATIGGNLLQRPRCWYFRNDKIDCWLKSGSDCPARDGENQLHALFPADSCVA
                     VHPSDPAAALLALDAELTVRGPGGERRIPLGDFFELPEDDRRTENTLRPDELIVAIHL
                     PGSAESVRSVYLKAMDRNVWAFALVGVALALRVDGSRIASARLVLSGVAPIPWRVPAA
                     EQLLEGADVGGFDVDQVTEAALADARPLSRNGYKLPLARALIKRTLTQVTGSPATT"
     gene            complement(2962..5274)
                     /locus_tag="VHR64_06585"
     CDS             complement(2962..5274)
                     /locus_tag="VHR64_06585"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3302533.1"
                     /translation="MSGFVVEKTGGGSGSKGQYTVKQPTPVEPWGAGAELRHVGQKHT
                     RVEGVEKVTGSARYAYDMHLPGQLVGRVLRSPHPHARVTRIDATKAEKLPGVHAVISS
                     ANTPEIPWYGDSFVFDPTVRFIGEEVVAIAAESEEIAGDALRLIEVDYELLPFVTELE
                     AALAPDAPKVRESGNIADTPTIYERGDVTAGFREAEATIDQTYTTQCALHNSFEPHGC
                     TASWEGDHLTLWDSTQSIFTVRDQVAKALDLPIQRVRVIKQFMGGGFGAKQISWKQDV
                     IAALLSKSSGRPVQLMLDREAENLAVGNRNPTHQRVRLGAKRDGTLTAIAVEIHQQAG
                     AHMTGGEASDVSAMYQTLYRCPNVRTDQTIVFTNTGPAVAFRAPGHVESAAALECAMD
                     ELARALEIEPIELRNRNYAGRDQQKDQPYTSPDSLRHCYEQVTRAFGWRSYRTPAPDG
                     PKRRGIGFAAHDWGGGGHPPGYAWVKLNPDGTADVVTGTQDIGTGTRTGLAQVAAEEL
                     GMAIEDVRIHLGDTGYGPYAPVSSGSATQATIGPAIRAAAFDAKRQLFEAAAVYLEST
                     PDKVSIHDGKLFVAGKPGHEGVPIGDVMTAIAPAMVMGQGAREPNPEDKSVGTFGAQC
                     IEVEVDTETGDVEVLRVVASHDCGRIVNPTMVDSQIIGAVMQGIGFALFEERVVDQRL
                     GIVLNANLEEYKVPTVADVPQIEHAAVDVPDPEANSTGAKGIGEPPLVPTAPAIANAV
                     YDAIGVRIRDFPLSRSRILAALADREGEQR"
     gene            complement(5271..5762)
                     /locus_tag="VHR64_06590"
     CDS             complement(5271..5762)
                     /locus_tag="VHR64_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3302534.1"
                     /translation="MEPTQVFVTFTLNGVQQAVAVDARRTLLDTLRSDLNLTGTKKVC
                     NMGDCGACTVLIDGRAVYSCLVLAADCAGREITTIEGIGRDGELDPIQQAFIENDAFQ
                     CGFCTPGQIMSLRALLDENPEPSDEEIERAVTGNLCRCGAYRNILAAGRQAVELQREG
                     AAR"
     gene            complement(5942..>7945)
                     /locus_tag="VHR64_06595"
     CDS             complement(5942..>7945)
                     /locus_tag="VHR64_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX3302535.1"
                     /translation="ARPGVTVDHVYTSAISGFAGTIDQAAAQALVNDSRVAGVFPDII
                     SHAASQSLPAGIDRVDADLNPTKAGDGSGTVDADIAVLDSGVADLPDLNLAGGMDCTN
                     SGSYADDNGHGTHVAGIAAARDNDQGVVGVAPGARIWAVKILHGNNFGYESEIICGLD
                     WVAAHSDVIDVANMSIQGFYGNIGNDCAAHPYHQAVCTVVSRGVTVVVAAGNADFTGI
                     PFDAAEIMPAQLNEVITVSAFYDKDGKPGGLAGSLDDTMAVFSNYGRDVDISAPGVNV
                     KSLNAAGGTIYMSGTSMASPHVAGGAALIRAQVGPLSPASVRGRLLLNGWSGPIPGDP
                     DGYPEPALDVAQLGLGTISAPASATPGSRIAVAVGQVVPGSRVTFRLNGTSIGVVTAG
                     ADGTATLRFKIPSMTRGSYAITVTSEQKQLRRTIRVLPYIRVSPVSGGVGSSVSVSLK
                     GFLGGESVLITFDAGAAIRRPVRVTTSASGAATAVFVIPSSVGGSRAVQAVGSRGTTA
                     STTFTVKPTVRISDSSLASTQSAELTVRGYDAGEVVTFRWDAADGPALGTKQTSSIGS
                     GIAAVALPDDASTGNHRIYAVGSGGHSASVGLSVTVAEEPVTPTATATITPPEGSPTE
                     AASPEPVSETPVVESPTEIATVTPTPSAAPTRDDGDGSPSPEP"
BASE COUNT         1262 a         2870 c         2461 g         1352 t
ORIGIN      
        1 tgccggcggg gccaatgcgt tgccctctgc ccgccggatc agatctactg cggcgatggc
       61 tgctacgacc cgctgaccga caacacgcac tgcggcgatt gcggggtgga ctgtggcggc
      121 ctgacctgct gcggcggggt ctgtatcgac acgaatgccg atccagcgaa ttgcggccag
      181 tgcggatccg cctgctccgc caacgcgacc tgcgaatcgg gccagtgcgt ctcgccttgc
      241 ccggccggta cgacgccatg cggatcaacc tgtgttgact tcacttcgga cccgtccaac
      301 tgcgggaatt gcggcaatgc ttgccccgcc gacagcacct gcaccaacgc aacctgcgta
      361 cccatctgcc ctgcgggctt cacgccatgc aacggcgcct gcgtcgacat cggcaccgac
      421 ccgcagaact gtggcgcctg cggtcagatc tgcgggagca aggcctgcca gaacgggacc
      481 tgccttgacg gtccgtgttt cgtctcgcaa gacggtccgg agctctcgtt cagatacgaa
      541 accgaatcgg ccgccgacaa cctctccctg cgcacgaccc gaacggtcct gagcgacgat
      601 tccggcgcaa ccctctcgat catcacccgg accaccgtct cccgcgaccg ccagaccgtg
      661 atccggatca accacgacta cgatgcgggc ggcgcatgga ccctgacgct ccactacggc
      721 gatgccttcg acgggatcaa ccgcgcggtc ttctccggtc agggcgattc cccgatcacc
      781 ggcaaggttg accgtcgccg gatctccccg ttcaccccgg gtgcggatcc atcgaccctc
      841 gcgttcgcgg acggcaagcc cgcgccaacc atcacggtcg accccgccct tgtcgatgcc
      901 ctcggccgca tcttcgccca ggccgagcag gagacaagcg cctgcacccc agtcgaccag
      961 accaacgcaa tcttgtcgcc gctcagctat ggcatcgcgg cgacggcccc gatcacgatc
     1021 tacgcccctg acatgcacgc cccgaccccc gccggcgccg gctactgcga tccaggtcat
     1081 cactcattcc cgcctctggg ttcggcaggc tgcgtcgcct gccggggcgg atgcctcgcc
     1141 gcgggcggcg tctgtggata ctcggctgcg gccggatgca gcgccggctg tgccctggcg
     1201 ctggctggat acggtgtgtg tctcggtatt tgcctggcgg ccgccctggt tgcgtgcgcg
     1261 gctggtgagc tcaagtgcca gaacgaatgc cgcaaaccgc cgggggcctg ctgcccatca
     1321 ggttgctctg atggctgctg cgacaacggc gagatctgcc tcgaccaggc cagtgggctc
     1381 tgctgcggcg gcgaattcac caagccatgc cagggcaagt gctgttgcag tcgcaccgac
     1441 aactgtcttc cggacggcac ctgctgccca cagggcaaca agatctgcaa cgacacctgc
     1501 tgcaattcgg gcgaaacctg cttcgagggc ggctgttgcg cgaaggtctg cgacaaggtc
     1561 acgccgcgca cctgctgcgg cgcgttcgat acctgcaccg agtgcggctg ctgcccaccc
     1621 ggcaccacct gcacgaccga caagtccgat cgcaagatct gctgcccgga tggccggctt
     1681 tgcggcgatg tctgctgtcc gaacggcgag gtctgccagg acccgcgcac cggcacctgc
     1741 ttcggctcta cctcgtgccc gccgggacag gtcgcctgcc cgggcggcac ctgctgtccg
     1801 acggtcaaag cccagggcgg cggcacgctc tacacctgct gcgggaacgt ctgctgtccg
     1861 tcggacaagc cctactgttg ctcgcccggc gacgacaccc acgccgcggt ctgcaacgcc
     1921 gtcggctgca tccggtagca catcccggcg acgacctgac acggaatcag gtcgtcgccg
     1981 ggcttccggt gacctgggtc agggtgcgct tgatgagcgc ccgcgcaagc ggcagcttgt
     2041 agccgtttcg gctcaatggc cgggcatcgg ccagcgccgc ttcggttacc tgatcgacgt
     2101 cgaatccgcc gacgtccgcg ccttccagga gctgctcggc cgcgggtaca cgccacggga
     2161 tcggcgcgac gccgctcagc accagtcgcg cactcgcaat ccgcgacccg tcgacccgca
     2221 acgccagcgc taccccgacc agcgcgaagg cccagacatt ccggtccatt gccttgagat
     2281 agacgctgcg caccgattcg gcggagcccg ggaggtgaat cgccacgatc agctcgtcgg
     2341 gccgcagcgt attctcggtc cggcgatcgt cctcaggcag ctcgaagaag tcgcccagag
     2401 gtatccgccg ttccccgccc ggaccgcgca ccgtcaactc cgcgtcgagc gcgagcagcg
     2461 cggcagccgg atcggacggg tgcaccgcca cgcatgagtc ggccgggaag agcgcgtgga
     2521 gctgattctc gccgtcgcgg gcgggacagt cggaaccgct cttgagccag cagtcgatct
     2581 tgtcgttgcg gaagtaccag cagcgcggtc gctggagcag attgccgccg atcgtcgcca
     2641 tgttccgtaa ttgcggcgtc gcggcgagtt cggccgcctg ggcaagcgcc atgtatcgcg
     2701 tccgcaagag ctcggagcgc tcgatctccg ccagtgtgca gagcgttccg atcgtcacac
     2761 cgtcctcgct ctcgtcgatc cgatccgaca gatccgaaac ccgcttgata tcgaccaggt
     2821 gcgacggcgt cgcgatctcc ggcttcatca atgtcagtag gtcagtcccg ccggcaagtg
     2881 ggcgcgtccc attgcccgat tcctgggaca ggatcgcgat agcctcggcc agactctcgg
     2941 cgctcgtgta ggcaaagggc ttcatcgctg ctccccttcc cggtctgcca gcgccgccag
     3001 aatccggctg cgcgagagcg ggaaatcgcg gatccggaca ccgatcgcgt cgtagaccgc
     3061 attcgcgatc gccggcgcgg tcggcaccag cggcggttcg ccgatcccct tggcgccggt
     3121 cgagttcgcc tccggatccg gcacatcgac ggccgcgtgc tcgatttggg gaacatcggc
     3181 caccgtcggc accttgtact cttcgaggtt ggcattgaga acgatcccga gtcgttgatc
     3241 gaccacccgc tcctcgaaca acgcaaaccc gatcccttgc atcaccgccc cgataatctg
     3301 gctgtcgacc atcgtcgggt tcacgatccg cccgcagtcg tgggacgcga ctacccggag
     3361 cacctcgaca tcgccggtct cggtgtcgac ctcgacttcg atgcactgcg cgccgaaggt
     3421 gccaaccgac ttgtcctccg ggttcggctc ccgcgcgccc tggcccatga ccatcgccgg
     3481 ggcgatcgcc gtcatcacgt cgccaatagg cactccctcg tgccccggtt tgccggcaac
     3541 gaagagcttg ccgtcgtgga tgctcacctt gtccggggtc gattcgagat agaccgcggc
     3601 cgcctcgaag agttgccgtt tggcgtcgaa cgcggccgcc cggatcgccg gcccgatcgt
     3661 cgcctgcgtc gcacttccgg agctgaccgg cgcatacggc ccatacccgg tgtcgccgag
     3721 atggattcgc acatcctcga tcgccatccc cagctcctcg gccgcgacct gcgccaggcc
     3781 ggtccgcgtg ccggtgccga tatcctgggt gccggtcacg acatcggccg taccgtccgg
     3841 attcagcttg acccacgcat agccgggtgg gtgaccacca ccgccccaat cgtgcgccgc
     3901 gaagccgatc ccgcgccgct tcggaccatc cggcgccggc gtccggtagc ttcgccagcc
     3961 gaatgcccgc gtaacctgct cataacagtg ccgcaggctg tcaggcgatg tgtacggctg
     4021 atccttctgc tgatcgcgac ccgcgtagtt gcgattccgc aattcgatcg gctcaatctc
     4081 cagcgcgcgg gccagctcgt ccatcgcgca ctcgagcgcg gcggccgact cgacatgccc
     4141 cggcgcccgg aacgcgaccg ccgggccggt gttggtgaag acgatcgtct gatccgtgcg
     4201 cacattcggg cagcggtaga gggtctggta catggcgctc acgtcgctcg cttcgccgcc
     4261 agtcatgtgg gcgcccgctt gctgatggat ctcgaccgcg atcgccgtca acgtgccgtc
     4321 ccgcttcgcc ccaagccgga cccgctggtg cgtcggatta cgatttccga cggccaggtt
     4381 ctccgcctcg cgatcgagca ttaattggac cggccggccc gatgacttcg agagcaaggc
     4441 ggcaatcacg tcctgcttcc atgagatctg cttggccccg aacccgccgc ccatgaactg
     4501 cttgatcacg cggacccgct ggatcggcaa atcgagcgcc ttggcgacct gatcccgtac
     4561 cgtgaagatc gactgcgtcg agtcccagag cgtcagatgg tctccctccc aactggccgt
     4621 acagccgtgc ggctcgaaac tgttgtggag cgcgcactgc gtcgtgtacg tctggtcgat
     4681 ggtcgcctcc gcctcccgga accctgccgt aacgtcgccc cgctcgtaaa tcgtcggcgt
     4741 gtccgcgatg ttgccgctct cccgtacctt cggcgcatcc ggcgcaagcg ctgcctccag
     4801 ctcggtcacg aacggcagca gctcgtagtc gacctcgatc aggcgcagcg cgtccccggc
     4861 gatctcctcg gactcggccg cgatcgccac tacctcctca ccgatgaacc gcaccgtcgg
     4921 atcgaagacg aagctgtccc cgtaccacgg gatctccggc gtattcgccg agctgatgac
     4981 ggcgtggaca cccggcagtt tctccgcctt cgtcgcatcg atccgcgtga cccgggcatg
     5041 cggatgcgga ctgcgcaaga cccggccgac cagttgcccc ggcaggtgca tgtcgtaggc
     5101 gtaccgggcg ctgccggtca ccttctcgac gccctcgacc cgggtgtgct tctgcccgac
     5161 atggcgcaat tcggccccag caccccatgg ctcgaccggc gtcggctgct tgacggtgta
     5221 ctggcccttc gatcctgatc ccccgccggt cttctcgacg acgaagccgc tcatcgcgcc
     5281 gctccttccc gctggagctc gaccgcctgc cgtccggccg cgaggatgtt gcggtacgcc
     5341 ccgcaccggc agaggttccc ggtcactgcc cgctcgatct cctcgtcaga cggttccggg
     5401 ttctcatcga gcagcgcgcg cagactcatg atctggcccg gcgtgcagaa gccgcactgg
     5461 aacgcgtcgt tctcgatgaa ggcctgctgt atcgggtcca gctcgccgtc tcggccgatt
     5521 ccctcgatcg tcgtgatctc ccggccggcg caatccgcgg ccagcacaag acaggagtag
     5581 acggcgcggc catcgatcag gacggtgcac gcgccgcaat cacccatatt gcagaccttc
     5641 ttggtgccgg tcaggttcag gtcactgcgc agggtgtcaa gcagcgttcg gcgcgcatcg
     5701 accgcgacgg cctgctggac gccattcagc gtaaaggtca cgaagacctg cgttggctcc
     5761 atggatggaa ctccagtggg catccggcga gggcggaggc gaccggttcg attgtgaggg
     5821 gctcgagttc gccggtatgc gcgtttgaat gactgaccga tcgccagcgc gcggcaaccc
     5881 tatcagcccg gcaatacccg gctgcgaacc tgccctacga tcaacgcgct gttcctggcg
     5941 cctatggctc tggagatggc gacccgtcac catcgtcacg tgtcggcgcc gcgctcggag
     6001 ttggcgtcac ggtcgcgatc tcggttggcg actcgaccac cggcgtctcg ctgaccggct
     6061 ccggcgatgc ggcctccgtc ggcgatccct ccggtggtgt gatcgtcgcc gtcgcagtcg
     6121 gtgttaccgg ctcctcggca acggtcaccg acaatccgac tgacgctgaa tgcccgccgc
     6181 tcccgaccgc gtagatgcgg tggttaccgg tcgacgcgtc gtcgggcaat gcgaccgccg
     6241 caatgccgct gccgatcgag ctcgtttgct tcgtcccgag cgccgggccg tccgctgcat
     6301 cccaccggaa cgtcacgact tcgccggcgt cgtacccgcg cacggtcagc tcggccgact
     6361 gcgtgctggc aaggctggag tcgctgatcc gcacggtcgg cttgacggtg aacgtggtcg
     6421 aggcggtcgt cccgcgcgag ccgaccgcct gcacagcgcg cgatccgccg acgctcgacg
     6481 ggatgacgaa gacggccgtc gccgcgccgc tcgcagaggt cgtcacccgc accggccgcc
     6541 gaatcgccgc accggcatcg aacgtgatca ggaccgattc gccgcccaga aaacccttca
     6601 gcgaaaccga caccgagctg ccaaccccac cggacaccgg cgacacccga atatatggca
     6661 gcacgcggat cgtccgccgg agctgcttct gctcgctggt caccgtgatg gcgtacgaac
     6721 cgcgggtcat ggacgggatt ttgaaccgca gcgtcgccgt accatccgcg ccggccgtca
     6781 ccacgccaat gctggtgccg ttcaatcgga acgtcacccg cgatcctgga acaacctgcc
     6841 cgaccgccac cgcgatccgc gaccccggtg ttgcgctcgc cggcgccgaa atcgtgccaa
     6901 ggccgagttg cgcgacatcc agcgccggct ccgggtagcc atcgggatcg ccgggaatcg
     6961 gcccgctcca gccattcagc agcagacgcc cccgtaccga cgccgggctc agcggcccca
     7021 cctgcgcccg gatcaatgcc gcaccaccgg ccacatgcgg ggaggccatg ctggtcccgc
     7081 tcatgtaaat cgtgccgccg gccgcattga gcgatttgac gttgacgccg ggcgcggaga
     7141 tatcgacatc gcgcccgtag ttgctgaaga cggccatcgt gtcatcgagg gaaccggcca
     7201 gtcctccggg cttgccatcc ttgtcgtaga acgccgagac agtgatcacc tcattgagct
     7261 gggccggcat gatctccgcc gcatcgaacg gaataccggt gaagtcggcg ttcccggccg
     7321 ccaccacgac cgtcacgccc cggctgacaa ccgtgcacac cgcctggtgg tacggatgcg
     7381 cggcgcagtc attgccgatg ttgccgtaga agccctggat actcatgttg gcgacgtcga
     7441 tcacgtccga atgtgccgcc acccagtcca acccgcagat gatctccgat tcgtagccga
     7501 agttattccc gtgcaagatc ttgaccgccc agatccgggc cccgggcgcc acgccgacga
     7561 cgccctgatc gttgtcgcgc gcagccgcga tcccggcgac atgggtgccg tgcccattgt
     7621 catccgcgta gctgcctgag ttggtgcagt ccatcccgcc ggccaggttg agatccggga
     7681 gatcggccac gcccgagtcg agcaccgcaa tgtcggcatc gaccgtcccg ctgccatcgc
     7741 cggccttcgt cgggttgaga tcggcatcca cgcgatcgat gccggccggg agagactggg
     7801 acgccgcatg cgagatgatg tccgggaaaa cgccggccac gcgcgagtca ttgaccagcg
     7861 cctgcgcggc cgcctgatcg atcgtgccgg cgaatccgct gatcgccgag gtgtagacgt
     7921 gatcgaccgt gacacccggt cgcgc
//
LOCUS       DATAOZ010000249         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6943068, whole genome shotgun sequence.
ACCESSION   DATAOZ010000249 DATAOZ010000000
VERSION     DATAOZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6943068"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VHR64_06600"
     CDS             <1..831
                     /locus_tag="VHR64_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX3302536.1"
                     /translation="RVDGLIGWISGKYLAPTDSWTEPDVSTDASTIAEDISSKIADLF
                     EAGSYVMASTDDGSGVNIRADGAPDAERIGIVPEGDVVQVMDGPSYDPIGNPWYLVTE
                     GDVTGFVSGWYLSRADQPGEDLTQAGLPSKLSIPGVATGTFEYPLASYTMTQGFGCSP
                     YYFEPWNGAVGCNFHNGVDLATDAYSPIYAADGGTVEYAGWCDCGLGYYVKIDHGNGF
                     ETLYGHMAEPPYVTAGQAVSKGDVIGPVGSTGMSTGPHVHFIVDYDGVDQDPLAYLPY
                     "
     gene            1085..2644
                     /gene="aceB"
                     /locus_tag="VHR64_06605"
     CDS             1085..2644
                     /gene="aceB"
                     /locus_tag="VHR64_06605"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX3302537.1"
                     /translation="MQINRPVDPAYQSILSPAALAFVADLHRRFNPRRRELLRARDER
                     QAEIDAGANLDFLPETLEVRQADWRVGPAPADLQNRRVEITGPCDRKMVINALNSGAK
                     VFMADLEDASSPTWNNVVEGQQNLYDAVRRTITFANPDGREYRLNPEIATLLVRPRGW
                     HLDERHMTVDGESVSGSLFDFGLYFFHNAKELIERGSGPYFYLPKLQSHREARLWNDV
                     FNAAQDALGIPRGTIRATVLVETLPAAFEMDEILYELRDHAAGLNAGRWDYIFSAIKT
                     LHNRDDRVLPDRAQVTMAVPFMRAYTQLLVKTCHHRGAHAIGGMAAFIPSRRDPEVNE
                     TAFVRVREDKEREAGDGFDGTWVAHPGLVPVAMEIFDRVLGERPNQKERQRDDVVADP
                     AKLVDLTVPNGRVTEAGVRNNVSVALQYLNAWLMGTGAAAINNLMEDAATAEISRAQL
                     WHWIHNDATTEDGEPITRQRYARIRAEELAKLGGADKERYGDAAEILDSLVLDNEFAP
                     FLTIPAYAYLD"
     gene            2648..>3337
                     /gene="uraD"
                     /locus_tag="VHR64_06610"
     CDS             2648..>3337
                     /gene="uraD"
                     /locus_tag="VHR64_06610"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX3302538.1"
                     /translation="MATPDPPWRLSEIASLPRDMFVERLGFLFEDSPWIVAEAWRSRP
                     WRTREQLHEALLQVVTVAGEARQVALIRAHPDLVGRAALAGSLSRESTAEQRAAGLDP
                     DALSEEDVQRFQEANAAYQARFGFPFVICARENKRASILAGFATRLGNDRAAEIDTAL
                     YEIGRIAWHRLADVVVDDTQEQRMSDPYQYVVTYGKYAVPVYRVFATPLEDVVPIPES
                     SFTGRQNNLLAC"
BASE COUNT          636 a         1043 c         1050 g          608 t
ORIGIN      
        1 cgggtcgatg gtctcatcgg ctggatctcg ggcaagtacc tggcaccgac tgatagctgg
       61 actgagcccg acgtgagcac agacgcatca accattgcgg aggatatctc ttcgaagatc
      121 gccgatttgt tcgaggcagg ctcctacgtc atggccagta cggacgatgg gtcaggagta
      181 aatattcgcg ccgacggcgc tcctgatgct gagcggatcg gtatcgtgcc ggaaggtgac
      241 gtcgtgcaag tcatggacgg tccctcctac gatcccattg gcaatccctg gtacctcgtc
      301 accgaaggcg acgtgactgg attcgtgagc ggctggtacc tctctcgcgc cgatcaaccc
      361 ggtgaggatc tcactcaggc gggtctgcca tcgaagctgt ccatacccgg tgtggccacg
      421 gggacgtttg agtacccgct ggcgtcatac acgatgacgc aaggcttcgg ctgcagcccc
      481 tactacttcg agccgtggaa cggtgcggtc ggatgtaact tccacaacgg ggttgacctg
      541 gccacggatg cgtactcccc catctatgcg gcagatggtg gcacagtcga gtacgccggc
      601 tggtgtgatt gcggcctcgg gtattacgtc aagatcgacc atggcaacgg cttcgagact
      661 ctttacggcc acatggcgga gccgccctac gtcacggccg gtcaggcggt ctcgaagggc
      721 gatgtcatcg gccccgtcgg gagcaccggt atgtcgaccg gtccccacgt ccatttcatc
      781 gtcgattacg acggcgtcga tcaggacccg ctcgcctact taccatactg atcgggtcta
      841 tctttcgccc ctcaccggtc gaccggcgag gggcgatttt gcgtgacagg caagtggtca
      901 ccatgagcgg gcggagagca gtccactcca ttgcgctatc atggccagac ggtccaacac
      961 ttatccatcg atcgcaaagt ccgaggcgaa gcccgtaggt cgatggtgag tgagttgccg
     1021 atccaatcaa ggcattgcac caggcccttt aagcgctaag gcggagttga ggatggattc
     1081 aggcgtgcag atcaaccgcc cagtcgaccc cgcgtaccag tcaatcctga gtcccgcggc
     1141 gttggcgttc gtcgccgatc tgcaccggcg attcaatccg cgccggcgtg aactgctgcg
     1201 agcgcgcgac gagcggcagg ctgagattga cgccggcgcg aatctcgatt ttctgcccga
     1261 gaccctggag gtgcgccaag ccgactggcg ggttggcccg gcgccggccg atctccaaaa
     1321 tcgccgcgtc gagatcacgg gaccatgcga tcggaagatg gtcatcaacg cgctcaactc
     1381 cggggccaaa gtcttcatgg ccgatctgga ggatgcttcc tcaccgacct ggaacaacgt
     1441 cgtcgagggg caacagaacc tctacgacgc ggtgcggcgc accatcacct tcgccaatcc
     1501 cgatgggcgg gaataccgac tgaatccgga gattgccacc ctcctggtga ggccacgtgg
     1561 ctggcatctc gacgagcggc atatgacggt cgacggtgag tcggtctcag gcagtctgtt
     1621 cgactttggt ctctacttct tccataacgc gaaagagctg attgagcgcg gcagcggtcc
     1681 ctacttctat ctgccgaagc tacaaagcca tcgcgaggcc cggctctgga acgatgtctt
     1741 caatgccgcg caggacgccc tcggcatccc gcgaggaacc attcgcgcga ccgtgctggt
     1801 cgagaccctg cccgcggcct tcgagatgga cgagattctc tacgagctgc gagaccatgc
     1861 ggcggggctg aacgccgggc gctgggatta catctttagc gccatcaaga cgctgcacaa
     1921 tcgggatgac cgcgtgctcc cggaccgggc acaggtgacg atggcggtgc cgttcatgcg
     1981 cgcctacacc cagctcctgg tgaagacgtg tcatcaccgc ggcgctcacg ccatcggcgg
     2041 catggccgcg ttcattcctt cccgccgcga cccagaggtc aatgagaccg ccttcgtcag
     2101 ggtgcgcgag gacaaggagc gagaagccgg cgatggtttc gacggcacct gggtggcaca
     2161 ccccggactg gtgccggtgg cgatggagat cttcgatcgg gtcctgggtg agcggccgaa
     2221 tcaaaaggaa cgtcagcgcg acgacgtggt ggccgacccg gccaaactcg tcgatctgac
     2281 ggttccgaac ggtcgggtca ccgaggccgg ggtgcgcaac aacgtcagcg tggcgctgca
     2341 gtacctgaat gcctggctaa tgggaaccgg cgccgcggcc atcaacaacc tcatggaaga
     2401 cgccgccacg gccgagatct ctcgcgctca gctctggcac tggatccaca acgacgccac
     2461 gaccgaagat ggagagccga taacgcgcca gcgctatgca cgtatccgtg cggaagagct
     2521 cgccaagctc ggtggcgcgg acaaagaacg gtacggggac gcagccgaga tcctcgacag
     2581 tttggtgctg gacaacgagt ttgcaccctt cctgaccatt ccagcgtacg cctacctcga
     2641 ctgacgattg gctacgcctg acccgccctg gcgtctcagc gagatcgcgt ccctaccacg
     2701 tgacatgttc gtggagcgac tcgggtttct gttcgaggac tcgccgtgga tcgttgccga
     2761 ggcctggcga agtcgcccgt ggcgaaccag agagcagctg cacgaggccc tgctgcaagt
     2821 cgtcacggtg gcgggagagg cgcggcaggt cgcgcttatc cgggctcatc cagatctggt
     2881 cggacgagcg gctctcgctg gttcgctgtc aagggaatcg accgcggagc agcgcgccgc
     2941 tgggcttgac cccgacgccc tatccgagga ggatgtccaa cgtttccaag aggccaacgc
     3001 cgcctaccag gcacgctttg gtttcccgtt cgtcatttgt gcgcgtgaaa acaagagggc
     3061 cagtattctg gccgggttcg caacccggct ggggaacgat cgcgccgccg agatcgacac
     3121 ggccctatac gagatcggcc gcatcgcctg gcaccgactc gccgatgttg tcgtagacga
     3181 cacgcaggag cagcgcatga gcgatcccta tcagtacgtg gtcacgtacg gcaagtacgc
     3241 cgtgccggtc taccgcgtct tcgcgacacc gctcgaggac gtcgtgccaa ttccggagtc
     3301 ctcattcacg gggcgccaga ataacctgct ggcctgc
//
LOCUS       DATAOZ010000250         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4579221, whole genome shotgun sequence.
ACCESSION   DATAOZ010000250 DATAOZ010000000
VERSION     DATAOZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4579221"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VHR64_06615"
     CDS             complement(<1..431)
                     /locus_tag="VHR64_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302539.1"
                     /translation="MRFGVLRRLLVVAALVALFAPMLAALSVPQIARADEDRPATYWD
                     ESQGVEIPGELGQSSIQVFVPQTKHTIRGYMLDYWRAQGAASVYGNPVSEPYGAKSGY
                     YSQAFEGAIFQFRPEYLWTETPSMTLEPIGQEVLQSRTGQIR"
     gene            complement(496..1350)
                     /gene="pyrF"
                     /locus_tag="VHR64_06620"
     CDS             complement(496..1350)
                     /gene="pyrF"
                     /locus_tag="VHR64_06620"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX3302540.1"
                     /translation="MAFAERLAAQIERANSLVCVGLDPDPTRFPQRFREMGRKQAIVE
                     FNRAIIEATSDLVCAYKPNLGFYAAQGLDGIEALLETRAMIPAEIPVILDCKVGDMGS
                     TATAYASAYFGEWKFEALTANPYQGEDSLAPFLAYPDRAVFILCRTSNPSSADIQDVA
                     LAESGEPLFMRVAGRANQWQRDYPAQVGLVVGATYPGELAMIRERSPKLPILLPGVGA
                     QAGDVEASVENGVTDQGDGLLVSSSRGIIYAGSGADFAERARAATASLRDQVNAVRAQ
                     RTGAAPAS"
     gene            complement(1362..3011)
                     /locus_tag="VHR64_06625"
     CDS             complement(1362..3011)
                     /locus_tag="VHR64_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX3302541.1"
                     /translation="MIGFPGQRSDLPWRERRIAPVMSTGGMVASAHPLVSSAGLRALA
                     AGGNAVDAAVSAALTASVCLPDMCGLGGDIFAIVHDPRQPRPVSYLGSGIAPRGLTYE
                     MAAMASPSGTLMPEQGPLSIGVPGMVRGYRDLLKNHGSKSFEELVQPALGYAEWGHPM
                     GRGEADHLGPAVGLLEKLESTRAVFLPEGKPIRTAELFIQSGLARTFRRLIEAGLDDF
                     YEGELARQIASSIQACGGVLSIEDLADHKTTVTEPISVEYRGYRINQTGLPSQGMIHL
                     EALRIADHALGAEPFWTDTAIHRQIESIKLAFADRLGYAQDSKTGDTPLDVLLSDEWA
                     RKRACEIGDCAATSVSAGKFQDGDTTYLCVVDGDGMMVSLIQSVSNAFGSGVIAGETG
                     IVMNNRVGRGFTLDTSHPNVYAPGKRTMHTLNCFSIEQLDGTPVLVGGTPGGDGQPQW
                     NLAMVSGLIDGKLDVQQVTEMPRWTVWPGTDPTGIGNPFELRLEDAFGPAMYEALARR
                     GHKIKAVAGWHGAAQIIARDPETGVIVGGSDPRVEGQAIGL"
     gene            complement(3060..>3438)
                     /locus_tag="VHR64_06630"
     CDS             complement(3060..>3438)
                     /locus_tag="VHR64_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302542.1"
                     /translation="APDAAGLGTVTASSTGSGSVAVRIPAEANEGSHDLWLIGNQGTT
                     VRLPLTIEAAVVPTPTTSATSTVEPTVTTPGGTPTIEPATETPVPTETATIAIPTETP
                     TTAAPTETQTPAPEGSAVDSGDT"
BASE COUNT          601 a         1131 c         1097 g          609 t
ORIGIN      
        1 cgaatctggc cggttcgcga ctgcagcacc tcctgaccga tcggttcgag cgtcatcgac
       61 ggcgtctcgg tccagagata ctcgggccgg aactggaaga tcgcgccctc gaacgcctgc
      121 gagtagtacc cgctcttggc gccatacggc tcggataccg gattgccgta gaccgatgcc
      181 gcgccttggg cgcgccaata gtcaagcatg tagccgcgga ttgtgtgttt cgtctgggga
      241 acgaaaacct ggatcgatga ttggccgagc tcgccgggga tctcgactcc ctgcgattcg
      301 tcccagtagg ttgcgggtcg atcctcatct gcgcgggcga tctgcgggac cgagagtgcc
      361 gccagcatcg gtgcgaacag ggcaacgagt gcggcaacca ccagcaaccg tcgaagaacg
      421 ccaaatctca tcgtgccaga ccaccccacc gctactgaaa actcacccgg acgcgcggaa
      481 cgcgtctctc tcctgctacg acgccggggc cgctccggtt ctctgtgccc ggaccgcgtt
      541 gacctgatcg cgcagtgaag cggtcgccgc acgagcccgc tcggcaaagt cggcgccgct
      601 tccggcataa ataatccccc gggacgacga tacaagcaac ccatcgccct ggtcggtcac
      661 tccattttcg acggaggcct cgacatcgcc cgcctgcgcg ccgacgcccg gcagcaaaat
      721 cggcaacttc ggggatcgct cccggatcat cgccagctcg cccggatagg tcgcgccgac
      781 caccaaaccg acttgcgccg ggtagtcccg ttgccactgg ttcgccctgc cggcgacgcg
      841 catgaagagg ggctcgcctg actccgcgag cgcgacatcc tgaatatcgg cgctgctcgg
      901 gttgctggtc cggcagagaa tgaagaccgc ccgatcggga taggcgagga acggggcgag
      961 gctgtcctcg ccctggtacg gattcgctgt cagagcctca aacttccact cgccgaagta
     1021 ggccgaggcg taggcggtcg cggtactgcc catgtcgcca accttgcagt cgaggatgac
     1081 cgggatctcg gccgggatca tcgcgcgggt ctcgagcagc gcctcgatcc cgtccaggcc
     1141 ctgcgcggcg tagaagccga gattcggctt gtaggcacac acgaggtcgg atgtcgcctc
     1201 gatgatggcg cgattgaact cgacgatcgc ctgcttgcgt cccatctccc ggaatcgctg
     1261 cgggaaccgg gtgggatccg ggtccagccc gacgcagacg aggctgttgg cgcgctcgat
     1321 ctgcgccgcg agtcgttcag cgaatgccac gccttccctc cctaaagtcc gatcgcctga
     1381 ccttcgacac gcggatcgct gccaccgacg atgacgccgg tctccggatc gcgcgcgatg
     1441 atctgcgctg cgccatgcca accggcaacc gccttgatct tgtgaccgcg ccgggcgagc
     1501 gcttcgtaca tcgccgggcc gaacgcatcc tcgagtcgga gctcgaacgg attaccgatg
     1561 ccggtcggat cggtgccggg ccagacggtc caacgtggca tctcggtgac ctgctggaca
     1621 tcgagcttgc catcgatcag cccactgacc atcgccaggt tccactgtgg ctggccgtcg
     1681 cctccggggg taccaccgac cagcacgggg gtgccgtcga gctgctcgat tgagaagcaa
     1741 ttgagcgtgt gcatggtccg tttgccgggt gcgtagacat tggggtgcga cgtatcgagt
     1801 gtgaagccgc ggccgacccg gttgttcatg acaatgccgg tctcgccggc gatcacgccg
     1861 cttccgaagg cattcgagac ggactggatc agcgaaacca tcatcccgtc gccatcgacg
     1921 acgcagaggt aggtcgtatc gccatcctgg aatttgccgg ccgagacgga ggttgccgcg
     1981 caatcgccga tctcgcatgc ccgcttcctg gcccactcat cgctgagcaa cacgtcgagc
     2041 ggggtgtcac cagtctttga gtcttgcgcg taaccgaggc ggtcggcgaa ggcgagcttg
     2101 attgattcga tctggcggtg aatcgccgtg tcggtccaga acggctcggc ccctagtgca
     2161 tgatctgcaa tccggagcgc ctcgagatgg atcatgccct gcgaaggcag gccggtctgg
     2221 ttgatccggt atccgcggta ctcgactgaa atcggctcgg tgacagtcgt cttgtgatcg
     2281 gccaggtcct cgatagacag gacaccgccg caggcctgaa ttgaggaggc aatctgccgg
     2341 gccagctcgc cctcatagaa gtcgtcgagc ccggcctcga tcaaacggcg gaaggtcctg
     2401 gccaaaccgc tctgaatgaa caattcggct gtgcggatcg gctttccttc aggcaagaag
     2461 acggccctgg tcgactccag cttctcgagc agtccgaccg ccggaccgag gtgatcggcc
     2521 tcgccccgtc ccatcggatg accccactcg gcatacccga gtgccggttg gaccagctcc
     2581 tcgaacgact tcgagccgtg gttcttcagg agatcacggt atccgcgaac catgcccggc
     2641 acgccgatcg agagcggacc ctgctcgggc atcaatgtgc cgctcgggct cgccatggcc
     2701 gccatctcat acgtcaggcc gcgcggggcg atgccactgc cgaggtatga gacggggcgg
     2761 ggctggcgcg ggtcgtgaac gatcgcgaag atgtcgccgc cgaggccgca catgtccggc
     2821 aggcagaccg aggcggtcag ggccgcgctg acggccgcat cgaccgcatt gccgcccgct
     2881 gccagagccc gcaggccggc gctcgaaacc aggggatggg ccgaggcaac catcccgccg
     2941 gtgctcatga ccggggcaat acggcgctcg cgccacggca gatcagatcg ttgtccggga
     3001 aatccaatca tcgtcatcca tcaatctgtc acgcaattcg atacattcgc gctagcgtac
     3061 tacgtgtcgc ccgagtcgac cgccgacccc tcaggtgcag gcgtctgcgt ctccgtcggc
     3121 gcggctgtcg tcggggtttc ggtcggaatc gcaatcgtcg ccgtttcggt cggaaccgga
     3181 gtttcagtcg ctggctcgat cgtcggtgtt ccgcccggtg tggtcacagt tggctcaact
     3241 gtgctcgtag cagaggtggt tggagtcggg acaaccgcgg cctcaatggt caggggcagc
     3301 cggacggtcg tgccctgatt accgatcagc cagaggtcgt gagacccctc gttcgcttcg
     3361 gctggaatcc gaaccgcgac actgccgctt ccggtcgatg aggcagtcac cgtgccaaga
     3421 ccggccgcat ccggtgcg
//
LOCUS       DATAOZ010000251         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7209844, whole genome shotgun sequence.
ACCESSION   DATAOZ010000251 DATAOZ010000000
VERSION     DATAOZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7209844"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VHR64_06635"
     CDS             complement(<1..298)
                     /locus_tag="VHR64_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3302543.1"
                     /translation="MSHARRFDHVGITVADLDTVTAFFVGLGLEVEGRTFVEGEFIDT
                     VIGIPDSRTEIVMLRPPDGGTRLELSSFVRPDHEPGSPAAMSNELGLRNVAFEVD"
     gene            complement(592..1146)
                     /locus_tag="VHR64_06640"
     CDS             complement(592..1146)
                     /locus_tag="VHR64_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX3302544.1"
                     /translation="MLIYSMSVSVDGFIADREGAFGWSAPSEEQFGFHLAQVRELGGY
                     LCGRGLYETMLVWETDPSLRGNELGAAFADVWCAIPKVVFSRTLGSVQGNARLAEASV
                     AEEAAAALDATDKDVSIGGTGLAAAAIELGLVDELRMFRYPVVVGGGTPFLPPVTEAV
                     PLDLIETRTFGSRVIYERYGRAPR"
BASE COUNT          325 a          574 c          530 g          275 t
ORIGIN      
        1 cgtcgacctc gaaggccacg ttgcgcagcc ccagctcgtt ggacatcgcg gccggtgacc
       61 ccggctcgtg gtcgggccgg acgaagctcg acagctccag ccgggtacct ccgtcgggcg
      121 gccgcagcat gacgatctcg gtgcgggagt cggggatgcc gatgacagtg tctataaact
      181 ccccctccac gaacgtccgc ccctcgacct cgagaccgag cccgacgaag aacgcggtca
      241 ccgtgtcgag gtccgcgacg gtgatgccga catggtcgaa gcgtcgtgca tgtgacatgg
      301 gatccatcct ccagtattcg acttgtcgat ccactccgag gacgtggccg atgggggtgg
      361 tctcgacatc ggccacgatg atttcgagac gcgtcacacc tcgtcgatcc gcgtctagcg
      421 tgccaacgat ttctcaatct cgctgggttg gatccggcga cgtatcgaag tgctcgaagg
      481 ccggttatcg tccggcggtt agccacatgg caagcgcgtc agagttggtc agcgcggcga
      541 atacccggtc cggcgaggcg ctgatgactc cgacggcagt gtcagtgcga ctcatcgcgg
      601 ggcgcgcccg tagcgctcgt agatcacgcg cgagccgaat gtcctggtct cgatcaggtc
      661 cagcgggacg gcttcagtga ccggcgggag gaacggcgtg ccgccaccga caacgaccgg
      721 atagcggaac atgcgcaact cgtcgacgag gccgagctcg atcgccgccg cggccaggcc
      781 ggtgccgccg atcgagacgt ccttatcggt cgcatcgagc gccgcggcgg cctcctcggc
      841 caccgacgcc tcggcgagcc gggcgttgcc ctggacgctg ccgagcgtgc ggctgaagac
      901 aaccttcggg atcgcgcacc agacgtcggc gaacgcggcc ccgagctcgt tgccgcgcag
      961 cgacggatcc gtctcccaca ccagcatcgt ctcgtaaagc ccgcggccgc acagatagcc
     1021 gccgagctcg cggacctgcg cgaggtggaa cccaaactgc tcctcgctag gggctgacca
     1081 cccgaacgcg ccctcccggt cagcgataaa gccgtccacc gagacgctca tcgaatagat
     1141 cagcatggct tcaagctcat cgcaccgaag cgacggacga tgatcgtgac gacgttcacc
     1201 attgagcttg acaccgggct ctcccacctt ctgcagtctg ctctgccact agcagtgacc
     1261 gatccaaaga caacactcat cgtcgtgagc ggtccaagcg atcgtacggc cagtgccggc
     1321 gccgcactga gatcccggaa gcggaacggg gggggggagc gcctcctgtc tactgcaaac
     1381 atccgaccag cgcgacgctt tcccgatctg tgcctcgcct catgagcggc cccagacctt
     1441 cggttggtcc acctccaggg ccggttgccc actttcgggc atcggtctct tattcgcgat
     1501 ccaggagctg gtcaagcctc agatgcgcct tgaatgcagc gtcaaaaaaa acaacatcgc
     1561 ggcccagcgt gtgccccacc gaaattgccg cggagattcc cgcaaaacgc aaatgacctg
     1621 gtcagagtgg gccccgtggg actcgaaccc acaacctacg gattaaaaag gcgaccgggc
     1681 tgagcgctgc gctgacgagg gaaa
//
LOCUS       DATAOZ010000252         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6155360, whole genome shotgun sequence.
ACCESSION   DATAOZ010000252 DATAOZ010000000
VERSION     DATAOZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6155360"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VHR64_06645"
     CDS             <1..315
                     /locus_tag="VHR64_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX3302545.1"
                     /translation="EDWMDSRPSLLAHPADQDSRTSTTLVAVIVDDDADHRMLRRIAV
                     SAHDAMARAIVPCHTLWDGDVVFVAQTGTTSETSQSDALRLSIATELAVESAIRSAIP
                     AS"
     gene            complement(395..796)
                     /gene="rpsI"
                     /locus_tag="VHR64_06650"
     CDS             complement(395..796)
                     /gene="rpsI"
                     /locus_tag="VHR64_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX3302546.1"
                     /translation="MTEKARYHYGTGRRKEAIARVRMYPGKGSITVNGKTVPEYFGGR
                     TNLHSALESPLRLTNTTGRYDIIVKVVGGGVAGQVGAVRHGIARALVRSDDELRPILK
                     RAKLLTRDARVKERKKVGLKRARKAPQYTKR"
     gene            complement(816..1253)
                     /gene="rplM"
                     /locus_tag="VHR64_06655"
     CDS             complement(816..1253)
                     /gene="rplM"
                     /locus_tag="VHR64_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX3302547.1"
                     /translation="MVDRRTFSPKAGDIQRDWFIVDADGLTLGRLASVIASTLRGKNK
                     PTYAPHMDMGDFVIVINAEKIKVTGNKETEKFYYRHSQYPGGLKATMLRDVRAKHPDR
                     IIKSAVRGMLPRTTLGERQLSKLKVYAGADHPHAAQQPKPLPL"
     gene            complement(1247..2104)
                     /gene="truA"
                     /locus_tag="VHR64_06660"
     CDS             complement(1247..2104)
                     /gene="truA"
                     /locus_tag="VHR64_06660"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX3302548.1"
                     /translation="MIDESARESVQSRLKLILAYDGTQFSGSQRQSGNRTVQGVLEEA
                     IASLINGPVKVAMAGRTDAGVHALGQVASCEDIRCDLEPTRLRLALNAQLPADVSVLE
                     VARAASTFDPRRDARWRRYRYRIWVGPRQPLMISQSLQMNGPLNVMRMLHGAVRFNGE
                     HDFASFAGLGMGVPGARGLGPRGTVRTIYRAHVRSRTSQEYRGQVIEIDIVGDAFLPG
                     QVRSMVGALLDVGRGKVQPDWIEELISAADRRRGPKAAPPHGLTLVEVGYEDWDPERI
                     SKRETGTLW"
     gene            complement(2106..2459)
                     /gene="rplQ"
                     /locus_tag="VHR64_06665"
     CDS             complement(2106..2459)
                     /gene="rplQ"
                     /locus_tag="VHR64_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX3302549.1"
                     /translation="MRHRKAGRKLGRNPAQRRALFRQLAVSMILHERITTTEAKAKTI
                     RPIVEKLVTIAREDTQAHRKLIMSKIDHPLATAKLFEVIAPRFTETPGGYTRIAKLEP
                     RRGDAAPMALIEFIA"
BASE COUNT          456 a          850 c          755 g          537 t
ORIGIN      
        1 gaagattgga tggactcgcg accgtcgctc cttgcccacc ctgcagatca ggattcgcga
       61 acgtcgacga cgcttgtggc agtcatcgtc gatgatgacg ccgaccatcg gatgctgcgt
      121 cgaatcgccg tgtcagcgca cgatgcgatg gcccgggcga tcgtgccatg ccacacgctc
      181 tgggacggcg acgtcgtttt cgttgcccag accgggacca cgagcgaaac gagtcagtcg
      241 gacgcgctac gtctctcaat cgcaactgaa ttggctgttg agtctgctat caggtccgcg
      301 atccccgctt cgtaacaagg gaagcgagag acgaaaaaga gccggcgcgt attgcgccgg
      361 ctctttcatg ttcataacgt ggcgaatcgg cagtttaccg cttcgtgtac tgaggcgcct
      421 tgcgggctcg cttgagaccg accttcttcc gctctttgac gcgagcgtcg cgggtcagca
      481 gcttggcgcg cttcaggatc ggccgcaact cgtcgtcaga gcggacgagc gcccgggcaa
      541 tgccgtgccg aacggcgccg acctggccag caaccccgcc accaacgacc ttgacgatga
      601 tgtcatagcg tccggtcgta ttggtcagcc gaagtgggct ctcgagggct gaatgcaggt
      661 tcgtgcgacc gccgaaatac tcggggaccg tcttgccatt aaccgtgatc gagcccttgc
      721 cggggtacat gcgaacccgg gcaattgcct ctttgcgccg gcccgtgccg tagtgatatc
      781 gcgccttctc tgtcatcgtt tgtcctcagt ctcccttaca gcggcagcgg cttcggctgc
      841 tgagccgcgt gcggatgatc cgctcctgcg tacaccttca gtttgctcag ctgccgctcg
      901 ccgagtgttg tgcggggcag cattcctcgc accgccgatt tgatgatgcg atccgggtgc
      961 ttggcacgca catcgcggag catggtcgcc ttcaggcctc ccggatactg agaatgccga
     1021 tagtaaaact tctcggtctc cttgttgccg gtaaccttga tcttctcggc attgataacg
     1081 atcacgaagt cgcccatgtc catgtgtggc gcataggtcg gcttgttctt gccgcgaagg
     1141 gtcgaagcga taacgctcgc cagccggccg agggtcaggc catcggcatc aacgatgaac
     1201 caatctcgtt gaatatcgcc agccttcgga gaaaaggttc gtcgatctac catagtgttc
     1261 ctgtctctcg cttgctgatc cgttccggat cccaatcttc gtatccgact tccacgagcg
     1321 tcagcccgtg tggtggcgcc gctttgggac ctcgtcggcg gtccgctgcc gatatcaact
     1381 cttctatcca gtcgggctgg accttacccc tcccgacatc gagcagtgct ccgaccatac
     1441 tgcggacctg tcctggcaga aacgcgtcac cgacgatatc gatctcgatg acctgcccgc
     1501 gatactcctg gctcgttcga ctccgaacgt gcgcccggta aatcgtccgg acggtgccgc
     1561 gcggtccgag accacgagcg cccggaacgc ccatgccgag ccccgcgaaa ctcgcaaagt
     1621 cgtgctcgcc gttaaaacga accgccccgt gcagcatgcg catgacattg agcggcccat
     1681 tcatctgcag gctctgcgag atcatgagcg gctgtcgagg cccgacccag attcgatacc
     1741 ggtagcggcg ccacctggca tctcgtcgcg gatcgaacgt cgacgctgct cgcgcaacct
     1801 ccagaaccga aacatcggcc ggcagctgag cattcagcgc cagccggagc ctcgtcggct
     1861 ccaaatcgca tcggatgtcc tcgcacgagg caacttgccc cagtgcgtgt acccctgcgt
     1921 ccgtgcgacc ggccatcgca accttgaccg gtccgttaat caagctggca atcgcctctt
     1981 ccaggacccc ctggacggtt cgattgccgc tctggcgttg cgagccagag aactgcgtgc
     2041 cgtcgtacgc gagaatcaac ttcagcctgg actggaccga ttcccgcgcc gactcgtcaa
     2101 tcaactcagg cgatgaactc gatcaatgcc atcggcgcgg catcgccacg gcgcggctcg
     2161 agcttcgcga tccgggtgta accgcccggc gtctcagtga agcgcggcgc gatcacctcg
     2221 aacagcttgg cggtcgcaag cgggtggtcg atcttcgaca tgatcagctt gcgatgcgct
     2281 tgggtatcct cacgcgcgat cgtgacgagc ttctcgacga tcggccggat ggtcttggcc
     2341 ttggcctcgg tcgtggtgat ccgctcgtgg aggatcatcg acaccgccaa ctgccgaaag
     2401 agggcccgtc gttgggctgg gtttcggcct aatttgcggc ccgccttacg gtgtcgcatc
     2461 gctacgcttc ctcgccattc gtctcgttca taccgagctc atcaccgcca agggccgcga
     2521 ttgcctcggc aacatcacgg ctctcgtcgt cctctccctc gtccatggcc gactcgagcg
     2581 catcgaagta gccgtgat
//
LOCUS       DATAOZ010000253         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_632295, whole genome shotgun sequence.
ACCESSION   DATAOZ010000253 DATAOZ010000000
VERSION     DATAOZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632295"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(787..862)
                     /locus_tag="VHR64_06670"
     tRNA            complement(787..862)
                     /locus_tag="VHR64_06670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(827..829),aa:Arg,seq:acg)
     gene            complement(1053..1142)
                     /locus_tag="VHR64_06675"
     tRNA            complement(1053..1142)
                     /locus_tag="VHR64_06675"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1104..1106),aa:Ser,seq:gct)
     gene            1320..>2864
                     /locus_tag="VHR64_06680"
     CDS             1320..>2864
                     /locus_tag="VHR64_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX3302550.1"
                     /translation="MTQRPARGAARLAAILDSLPDALLLVDMRGLVVNANARALEMFE
                     TQAGPLVGRALTELVPGFDRTKAAGRSRRRKERRGERGKPERYSARQTDGSSFACDVS
                     TSLLSGTAEDELLVVVVRRLDDQIESSAEMTRQRKQTETILRATSEAIIGVDNNGKII
                     LANPAAARMLRTKATELAGKDLHDLAHHSRPDGSPYPRSSSPLIETLRTGKRTKSREE
                     ILWRRDGSQILVEMSTSPVMENETLTGAIVAYVDRGDLLVAKRRREELVHVLRRELGR
                     PLGRARNDIARVAASEYGPVSPEAAGALDEIAAGFDRLIRVISQALDQDRNEPSRAAF
                     TQRADALKLVQLAVDAVTPAVDDAGVFVSVDADPIEIDIDEERLVQALATVLSSAARS
                     APPQSTVTITAKQRGDRLRIVVRGTGTGMPPRIVERPAGSSTAIDVGMPVARRLVDQL
                     GGSMTVYSSPADGTSCTIELPISVRSVEPQSGGPAPPAIIPPQDLPESEADLTEPAGD
                     VDLPDLP"
BASE COUNT          532 a          940 c          942 g          450 t
ORIGIN      
        1 gcctccggct gatcgtttcg cggagacaac ccagtgatag acgctattcc tgtggtttag
       61 cggtgtaatg gcagatcctg catattttgc caccctcggg gccggatgtc cgacctcagt
      121 gcgatctgct gtgccctgcc caaatcggag gaaggtttct gacgcctcgc cgataaatgg
      181 cgtgctgcgc accgtggctg cggacgggct acgagaggcg gtcgacaggc ccgggcgagt
      241 tctcagcggt acacgcagcg ccccggcata caggaccggt gccccgttcg tcgatgtcgt
      301 tggtcccgcg atcggcgacc agggccagca cgtcgatgcg cggctcgatc cgcaagcctc
      361 ggtggtcgac tcggactggc acccatcgtc aagagccgga cggtccaggt cggcgatcgt
      421 ctgtggtacg cggccgtgcg gccgtcctcg atcagttcac gatagttgcg ttcgagcagc
      481 gtcggcgggt cccgcaacac ctcggcatcg cgcaggcttt cgcatcgaga tcgtccgtag
      541 ttgggacgcg ctggagccaa tccgcgtccg ttggagttga gtgagaggag gtgatcaaca
      601 tgggattcgt cgaggagccg acgcacgtca tcgacgtgcc ggagccgctg gagatcccgg
      661 acccagcagc gcccgcggag gaaccggagg aggtgccagc gtgaccacaa acgaatcgca
      721 aagtgagacg ttgtgagatc tgatcttgcg tggccgatca ctcgatcaag ggactgcctg
      781 gtcaggtggc gcgctcggag ggattcgaac ccccaacctt ctgatccgta gtcagatgct
      841 ctatccgttg agctacgagc gcacgtcgga aaaccgacgg ttgagtctac aggccagaag
      901 gcgaccgccg gaaaccgaaa gctctgcccg agcaaccggt gcggcggcct tattccccca
      961 gctcagttgg ctcggcgcga tccagaggtg cttgacacag ccaccgggcc ggccgcgccg
     1021 tgggcacggc cggcatcgcg acgagtgagg ggcggaggct ccgggatttg aacccgggag
     1081 gggctttgaa ccccaaccgc attagcagtg cggcgccata gaccagacta ggcgaagcct
     1141 cccgacctgc ccgataaaca tgatcgccca ccggcccgcg gcaggctacc aatcgtcaac
     1201 cgggcgtgct caggttaccg ggccgctcaa catcccggca aagtcgtggg acactctctc
     1261 actcagagag catcgtcagc ccgactgcgg ccccgaccag gagggagtgt gtgacgcccg
     1321 tgacccaacg cccggcccga ggggctgcca ggttggcggc gatcctggac tcgctacccg
     1381 acgcactgct cctggtcgat atgcgcggcc tggtcgtcaa tgcgaatgca cgcgcgctgg
     1441 agatgttcga aacgcaggcc gggccgctcg ttggccgagc gctgacggag ctggtgcccg
     1501 gttttgaccg gacgaaggcg gcaggccgct cgcggcgccg caaggagcgg cgaggcgagc
     1561 gcggcaagcc cgagcgctac tcggccaggc agaccgatgg cagcagcttc gcgtgcgatg
     1621 tctcgacttc gctcctctcc ggtacggcag aagacgagct cctggtggtc gtcgtccgcc
     1681 ggctcgacga tcagatcgag tcaagtgccg agatgacgag gcagcgcaag cagacggaga
     1741 cgattctgcg ggcgacctcc gaagcgatca tcggcgtcga caacaacggc aagatcatcc
     1801 tcgccaatcc ggcggccgca cggatgctca gaaccaaggc caccgagctc gctggaaaag
     1861 acctccacga cctcgctcac cacagccgcc cggacggatc gccctaccca cgaagctcat
     1921 cgccgctgat cgagacgctg cggaccggca agcgaaccaa atcccgcgag gagattctgt
     1981 ggcggcggga cggttcacag attctggtgg agatgtcgac ctcgccggtg atggaaaacg
     2041 agacgctgac cggcgccatc gtcgcctacg tcgaccgggg cgatcttctc gtcgccaagc
     2101 gacgtcgaga ggaactcgtc cacgtcctgc gccgcgaatt agggcgtcca ctcggtcggg
     2161 ctcgcaacga catcgcgcgt gtcgccgcat ccgagtacgg gccggtctca cccgaagcag
     2221 ctggagcgct ggacgagatc gcagccggct ttgaccgact gattcgcgtc atcagccaag
     2281 cgctcgacca ggaccgcaac gagccaagcc gcgccgcctt cacgcaacgc gccgatgcac
     2341 tgaagctcgt ccagttggcc gtggacgcgg tcacaccggc cgtcgacgac gcgggcgtct
     2401 tcgtgtcggt tgacgccgac cccatcgaga tcgatatcga cgaggaacgg ctcgtccagg
     2461 cactcgcaac cgtgctcagc tcggccgctc gatcggcacc gccacagtcg acggtgacca
     2521 taaccgccaa gcagcgcggc gaccggttgc gcatcgtggt acgcggcacc ggcacgggaa
     2581 tgccaccacg gatcgtcgaa cgtccggcgg gctcgagcac cgcgatcgat gtgggtatgc
     2641 cggtcgcacg gcggctcgtc gaccagcttg gtggttccat gacggtctat agctcgccgg
     2701 ccgacggtac gagctgcacg atcgagctgc cgatttctgt gcggtcggtc gaaccgcaga
     2761 gcggtgggcc ggcacctcca gcgatcatcc caccgcagga tctgccggag agcgaagcag
     2821 atctgaccga gcccgcgggt gacgtggacc tccccgacct tccc
//
LOCUS       DATAOZ010000254         7035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2213190, whole genome shotgun sequence.
ACCESSION   DATAOZ010000254 DATAOZ010000000
VERSION     DATAOZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213190"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VHR64_06685"
     CDS             complement(<1..908)
                     /locus_tag="VHR64_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX3302551.1"
                     /translation="MPWHDVTFDLAFANGAVLLAGIVLYALLGGADFGGGIWDLLARG
                     PRADQQRQEIARAIGPIWEANHVWLIFSLVTAFTVFPKVFAIVCIALYVPLSLTLLGI
                     VLRGAAFVFRAYAHDVTLAQQGWGGVFAISSTVTPLLLGMCAGAVASGEIRVVDGNFE
                     GSYWTTWLSPFPMVIGLLSLTTCAYLAAVYLTIEARGKGDLEEDFRRRALGAGTVFTV
                     LSLIALPLARSDAPQIWHGMADREVWTVVPVGIALAAVSGWAVWTRRYGLARAAAVVE
                     VAVLIVGWALAQYPYLVVPDLTYENTA"
     gene            complement(895..2235)
                     /locus_tag="VHR64_06690"
     CDS             complement(895..2235)
                     /locus_tag="VHR64_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX3302552.1"
                     /translation="MDNLLAARLQMASSLAFHIIFAGLGIGLPLLMVLAEGLWLRTGN
                     IAYLALARRWSKAFAMIFAVGAVSGTILSFELGLLWPRFMEFAGGIIGMPFSAEGFAF
                     FIEAIFLGIYLYGWDRLSPRAHWLTGIPVAVSGLLSGIFVVTANAWMNSPAGFRIVNG
                     QVVDVDPIAAMFNDAWFQQSLHMSLAAYHATAFAVAGVYAWGMLRGRNDAYHRAGLTV
                     ALSVATLFAVLQPISGDISARWVAENQPVKFAALEGQFQTERHAGLRIGGIPDPKARE
                     TRYALEIPGLLSFLAHGNTDAEVKGLNEWPLADQPDPRIVHLGFQTMVGIGFSVIALC
                     AWFWLTVWRRKQIAPGRWLLRALVLATPAGFIAIEAGWTVTEVGRQPWVIQGVMRTEN
                     AVTTVPNQFVALGGFTFVYIILATALVWLLLRLSRTPPAMEEPRTRREVRRAVA"
     gene            2425..3369
                     /locus_tag="VHR64_06695"
     CDS             2425..3369
                     /locus_tag="VHR64_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302553.1"
                     /translation="MVAALHLATMSVEQPAAIATYNLTKSYGANRGIRDINLRVERGE
                     IFGFLGPNGAGKSTTLRTILGFLRPTSGRAEILGLDAQHQTVEIHRNLGNLPSEFSLW
                     DRMTGAEMLRLFADLRGGDIPLDYAHELAERLDADLTRPMRRLSRGNKQKIGIIQALY
                     HQPPVVVLDEPTTGLDPLMQDVFLDILRETRDRGQTVFFSSHILPEVEQVADRVGVIR
                     EGELIAIEDPQALTSKGFRHVRIQFAEPLGAEELAQLAAMPGVDRFKVSGATVTFTAH
                     GSMDPIIKLASRHRIELFDAERPPLEEVFLSFYGESAA"
     gene            3366..4151
                     /locus_tag="VHR64_06700"
     CDS             3366..4151
                     /locus_tag="VHR64_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3302554.1"
                     /translation="MKSTLATYLAFELRSNLRSTIYWTIGLSIYVGTVVSLYPSVKSA
                     LNISAIPPNLRAAFNITDFTYLAGFLSSELFGVILPLVLPFYGIVILSNVVAGNEERG
                     RLDVLLGNPIPRWQLVAGSFIVVASYMLAMVMVFGSVIWGVASALDLALSAREAYRAT
                     FALWPPALAFGTLALAVSTRARQRAVAIGIPGAVVLLSYLILVIGRLADALSFVKYIS
                     AYYYYGFAILQGIWWGGVTVLLSATTILLAIAIITFNRRDIYA"
     gene            complement(4427..6130)
                     /locus_tag="VHR64_06705"
     CDS             complement(4427..6130)
                     /locus_tag="VHR64_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX3302555.1"
                     /translation="MSVLRPSSVRYLNDQSVPMRDGVRLSADIYLPDVGEGPWPVLLA
                     RSPYDNNLLWEVGCFWAQNGYVYVAQDVRGRYDSDGAFVPWVNETDDGFDTIEWIGRQ
                     SWCNGNVGMTGASYLGQVQWQAAFTQHPLLKAICPRVMGNNLWDSPHYQGGAFGLGVN
                     AVWGWRTMARTTQRIDKFDWPAILRTLPLRLMDKITGKQHPAFGTWLDHEAYDDYWRA
                     TAVDEHFERFTIPVLQVCGWYDLYAGGMMANFVGLREHAGSELARENQQIIMGPWTHP
                     QAGLHFPPGTTNAGDRDFGFGSLLDTKTIELAWFDHWLKGVENGAEKRPPVTIFVIGA
                     DTWRDEQEWPLARTEWTPYYLHSGGAANTLYGDGTLSIDLAAAEPPDTYAYDPEHPVP
                     TLGGCNCCNPEVIPWGVYDQRPVEVRGDVLVYTTPPLEQDLEVTGPIVVHLFAATDGP
                     DTDFTAKLVDVYPDGKAWNLCDGIVRGRYRKGRESAAELLTPGELYAFEIDCWVTSNL
                     FKAGHRIRLEISSSNFPRFDRNLNTGNPIGDDNQPRIATNRIYHDADHPSHILLPVIP
                     G"
     gene            6355..>7035
                     /locus_tag="VHR64_06710"
     CDS             6355..>7035
                     /locus_tag="VHR64_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX3302556.1"
                     /translation="MNGKKADGIRVSRRGVVKGGAAAAAVGAVAAAPHLGGNQHAQAQ
                     SAEDIANARGLTPQDMTAALKTYVPSGKLDEYYIFASGGHGGQVLVIGVPSMRLLKVI
                     GVFTPEPWQGYGFDDGSKSVLANGGMNGKTLTWADTHHPGLSETNGEYDGQFLFINDK
                     ANSRLAVIDLRDFETKQIVKNPISIDDHGFCVTPDTEYVLEGAQYATPLGWKYAPIED
                     YQKSYGGTL"
BASE COUNT         1260 a         2280 c         2317 g         1178 t
ORIGIN      
        1 gccgtgttct cgtaggtcag gtcgggcacg accaggtatg ggtactgggc cagggcccag
       61 ccgacgatca gcaccgcgac ctcgacgacc gccgccgccc gggcgaggcc gtaccgccgc
      121 gtccagaccg cccagccgct gactgctgcc agcgcgatgc cgactggcac gacggtccag
      181 acctcgcgat cggccatgcc gtgccagatt tgcggtgcat cgctccgggc cagtggcaag
      241 gcgatcagtg acaggaccgt gaagaccgtc ccggcgccga gcgcgcgacg gcggaaatcc
      301 tcctccaggt cgcccttgcc tcgtgcttcg atcgtcaaat agaccgcagc caggtaggcg
      361 caggtcgtga gggagagcag cccgatcacc atcgggaagg gcgagagcca ggtcgtccag
      421 tagctgccct cgaagttgcc atcgacgacg cgaatctctc cgcttgcgac cgccccggcg
      481 cacatcccga gcagcagcgg cgtcaccgtg ctggagatcg cgaagactcc gccccaaccc
      541 tgctgggcga gcgtgacatc atgcgcgtag gcccggaaga cgaaggcggc gccgcgcagg
      601 acgatgccga gcagcgtcag ggagagggga acgtacagtg cgatgcagac gatggcgaag
      661 accttgggga agaccgtgaa ggccgtgacg agcgagaaga tcagccagac atggttggcc
      721 tcccagatcg gcccgatcgc gcgcgcgatc tcttgccgtt gctgatcggc ccggggaccg
      781 cgcgcgagca aatcccagat cccgccgccg aaatcggccc cacccagcag ggcatagagc
      841 acgatcccgg ccagcagaac cgcgccgttc gcgaaggcga gatcgaaggt gacgtcatgc
      901 cacggcacgg cgcacctccc gtcgcgttcg cggctcctcc atcgccggcg gcgtgcgcga
      961 gagccggagc aggagccaga ccagcgccgt tgccaggatg atgtagacga aggtaaaccc
     1021 gccgagcgcc acgaactggt tcggcacggt cgtgaccgca ttctcggtcc gcatcacgcc
     1081 ctggataacc cacggttgcc ggccgacctc ggtcacggtc caaccggcct cgatcgcgat
     1141 aaacccggcc ggtgtggcga ggacgagcgc gcgcaacagc cagcggccgg gcgcgatttg
     1201 cttccggcgc cagaccgtga gccagaacca ggcgcagagg gcaatcacgc tgaagccgat
     1261 gccgaccatc gtctggaagc cgaggtggac gatgcgcggg tcgggctgat cggccagcgg
     1321 ccattcgttc agccctttga cctcggcatc ggtattgccg tgggccagga agctgagcag
     1381 gcccggaatc tcgagcgcat atcgggtctc tcgcgccttg ggatcgggga tgccgccgat
     1441 gcgcaacccg gcgtgtcgct cggtctggaa ctggccctcg agcgcggcga atttgaccgg
     1501 ctgattttcg gccacccagc gcgcgctgat gtcgccgctg atcggctgga gcacggcgaa
     1561 gagagtcgcg accgaaagcg cgaccgtgag ccccgcccgg tgataggcat cgttgcggcc
     1621 gcggagcatg ccccaggcgt agaccccggc cacggcgaag gcggtcgcat gataggcagc
     1681 gagcgacata tgcagcgatt gctggaacca ggcgtcgttg aacatggccg caatcgggtc
     1741 gacgtcgacc acttgcccat tgacgatccg gaagccggcc gggctgttca tccaggcgtt
     1801 ggccgtgacc acgaagatgc cggaaagcag accgctgacc gcgacgggga tgccggtcag
     1861 ccagtgtgcc cggggcgaga gacgatccca gccgtagagg tatatgccga ggaagatcgc
     1921 ttcgatgaag aaggcgaatc cttccgccga gaagggcatg ccgatgatcc cgccggcgaa
     1981 ctccatgaag cggggccaga gcagaccgag ttcgaaggag agaatcgtgc cggagacagc
     2041 gccgaccgcg aagatcatcg cgaacgcctt ggaccagcgc cgggccagcg cgaggtaggc
     2101 gatgttgccg gttcgcagcc agagtccctc ggccagcacc atcaggagcg ggagaccgat
     2161 cccgaggccg gcgaagatga tgtggaatgc cagcgacgat gccatctgga gtcgcgccgc
     2221 cagcagattg tccaccgcac gcctcctgtc tccggcagga atcctgccag gtcccggaac
     2281 agggcgtggt gcggcgttga atcggtgaag tgtccccgcc cgggcatacc caagcgttca
     2341 tgatcagcat aggccgatcg ccagtccttt gtctgtcggg gaattgccga cgatgccgga
     2401 tggaaatgcc tgacgcgcgc cgggatggtt gccgcactac acttggcgac aatgtcggtc
     2461 gagcaacccg cggcgattgc cacctacaac ctgaccaagt cgtatggcgc gaatcgcggc
     2521 attcgcgata tcaacctgcg cgtcgagcgg ggcgagatct tcggcttcct cggtcccaac
     2581 ggcgccggca aatcgaccac cctgcgcacg atcctcggtt tcctccgccc gaccagcggc
     2641 cgcgccgaga tcctgggcct ggacgcgcag caccagacgg tcgagatcca ccgcaacctc
     2701 ggcaacctgc cgagcgagtt cagcctctgg gaccggatga ccggcgcgga gatgctgcgc
     2761 ctcttcgccg atctgcgcgg cggcgatatc ccactcgact acgcccacga actcgccgag
     2821 cgactcgatg ccgacctgac ccggcccatg cgtcgcctct cgcgcggcaa caagcagaag
     2881 atcggcatca tccaggcgct ctatcaccag ccaccggtgg tcgtgctgga tgagccgacg
     2941 accgggctcg atccgctgat gcaggacgtc ttccttgata tcctgcgcga gacgcgggac
     3001 cggggtcaga cggtcttctt ctcctcgcac atcctgcccg aggtcgagca agtggccgat
     3061 cgggtcgggg tgatccggga gggtgagcta atcgcgatcg aggatccaca ggcgctgacc
     3121 agcaaggggt tccggcacgt ccggatccag ttcgccgagc cactcggtgc cgaggagttg
     3181 gcccagcttg cggcgatgcc cggtgtcgac cggttcaagg tcagtggcgc gaccgtcacc
     3241 ttcaccgcgc acggctcgat ggacccgatc atcaagctgg ccagccggca ccggatcgag
     3301 ctcttcgatg ccgagcggcc gccgctggaa gaggtcttcc tcagctttta cggcgagtcg
     3361 gccgcgtgaa atcgaccctc gccacctatc tcgccttcga gctccgctcg aatcttcgct
     3421 cgactatcta ctggacgatc gggctgagca tctacgtcgg cacggtcgtc tcgctctacc
     3481 cgtcggtcaa gtcggccctg aatatcagtg cgatcccgcc gaacctgcgg gcggcgttca
     3541 acatcaccga cttcacgtat ttggccgggt ttctgtccag cgagttgttc ggcgtgatcc
     3601 taccgctggt gctgcccttc tacggaatcg tcatcctctc gaatgtggtc gccgggaatg
     3661 aggagcgggg ccggcttgat gtcctgctcg gtaacccgat cccgcgctgg cagcttgttg
     3721 ccgggagttt catcgtggtc gcgagctata tgctggcgat ggtgatggtc tttgggagcg
     3781 tgatctgggg cgtggcgtcg gcgctggatc tcgccctttc ggcgcgggag gcctaccggg
     3841 cgaccttcgc gctctggccg ccggcgctcg ccttcgggac gctggcgctg gccgtctcga
     3901 cccgggcccg gcagcgagcg gtcgcgatcg gcatcccggg cgcggtggtg ctgctcagct
     3961 acctgattct cgtgatcggg cggctggcgg acgcgctctc gttcgtgaag tacatctcgg
     4021 cctattacta ctacggcttc gcgatcctcc aaggcatctg gtggggcggc gtcaccgtgt
     4081 tgttgtccgc gacgacaata ctcctggcca ttgccatcat caccttcaac cgccgcgaca
     4141 tctacgcgta gggagtcgag aagtcgagca gttagggcgc ggagacggga aggcgaatgt
     4201 agggatttgc cgtggcaaat ccgcccgcgt ccgcaccgaa ctcgccctgc gcggaatacc
     4261 tgactggtac tggtcacagg atcggaagcc aggcgcggat ttgccacggc aaatccctac
     4321 agagatcgtg ttgatgcatc tgcctgaccg gtacgcacgt tcccgaaccc cgaagcccga
     4381 actccgaacc ccgacagacc aaaacccaag acccaaaacc caaaacctac cccgggatca
     4441 ccggcagcag gatgtgcgac gggtgatcgg cgtcgtggta gatgcggttg gtggcgatgc
     4501 gcggctggtt gtcgtcgccg atcgggttgc cggtgttgag gttgcggtcg aagcgcggga
     4561 agttgctcga tgagatctcg agccggatcc ggtgcccggc cttgaacagg ttcgacgtca
     4621 cccagcagtc gatctcgaac gcgtacaact cgcccggagt cagcagctcg gccgctgatt
     4681 cccggccctt ccggtacctc ccgcgcacga tgccgtcgca gagattccag gccttgccat
     4741 ccgggtagac atcgaccagc ttggcggtga aatcggtgtc cggaccgtcg gtcgcagcga
     4801 agaggtggac gacgatcgga ccggtcacct ccagatcctg ctccagcggc ggcgtggtgt
     4861 agacgaggac atcgccccgg acctcgaccg gccgctgatc gtagacgccc cacggaatca
     4921 cctccgggtt gcagcagttg cagccgccga gcgtcggaac cgggtgctcc gggtcgtagg
     4981 cgtaagtatc gggcggctcg gcggccgcga ggtcgatgct gagcgtgccg tcgccgtaga
     5041 gcgtattggc cgcgccgccg gagtgcaggt agtacggcgt ccactcggtg cgggccaggg
     5101 gccactcttg ctcgtcgcgc caggtatcgg cgccaatcac gaagatcgtg accggcggcc
     5161 gcttctccgc cccgttctcg acgcccttta gccagtggtc gaaccaggcc agctcgatcg
     5221 ttttggtgtc gagcagcgag ccgaaaccga aatcgcgatc gccggcgttg gtcgtgcccg
     5281 gcgggaagtg caatccggcc tgggggtggg tccacgggcc catgatgatc tgctggttct
     5341 cgcgggccag ctcagacccc gcgtgctccc ggagtccgac gaagttggcc atcatcccgc
     5401 cggcatagag gtcgtaccag ccgcagacct gcaggaccgg gatcgtgaat cgctcgaagt
     5461 gctcgtcgac ggcggtcgcg cgccagtagt cgtcgtatgc ctcgtggtcg agccaggtcc
     5521 cgaaggcggg gtgctgcttg ccggtgatct tgtccatcag ccgcaacggc agcgtgcgga
     5581 gaatggccgg ccagtcgaac ttgtcgattc gctgggtggt ccgggccatg gtccgccagc
     5641 cccagaccgc gttgacaccg aggccgaacg cgccgccctg gtaatgcggg ctgtcccaga
     5701 ggttgttgcc catcacccgc ggacagatcg ccttgagcag tgggtgctgc gtgaaagctg
     5761 cctgccactg gacctgcccg aggtaggagg cgccggtcat cccgacattg ccgttgcacc
     5821 aggactgtcg gccgatccac tcgatcgtgt cgaagccgtc gtcggtctcg ttgacccagg
     5881 gcacgaaggc gccgtcggag tcgtatcggc cgcggacatc ctgcgcgaca tagacgtagc
     5941 cgttctgcgc ccagaagcat ccgacctccc agagcaggtt gttgtcgtag ggcgatcgcg
     6001 ccagcaggac cggccacggc ccttcgccca cgtccggcag gtagatatcg gccgagaggc
     6061 gtacgccatc tcgcatcggc acggactggt cgttgagata gcggacgctc gacggtcgca
     6121 gcacggacat gcagtcatct ccccgggtgg ctcggtggtc ggtcgccgca ggataccgaa
     6181 ttccgcaact cctcgttcgg ccatcccagc ttcagaatcc tgcgtacgaa cgccgattgt
     6241 cacgattgtg taatgcccct gaccgggaat tgacagcttt ggaattgcca gtgatctagt
     6301 ctgatttcgt cgcgtgttgc gaacgttgtt gcttacttgg aggtcatttg cgacatgaac
     6361 gggaagaagg ctgatggcat ccgtgtctca agacgcggcg tcgtcaaggg aggggcagcc
     6421 gccgcggccg ttggcgcggt tgccgcggca ccccatctgg gcggcaacca gcatgcgcag
     6481 gcgcaatccg ctgaggatat cgccaatgcg cgtgggctca ccccccagga catgacggcc
     6541 gcgctcaaga catacgtgcc cagcggaaaa ctggacgagt actacatctt cgcctcgggc
     6601 ggacatggcg ggcaggtgct cgtcattggc gtaccctcaa tgcggctgct caaggtaatc
     6661 ggtgtcttca cgcctgagcc gtggcaggga tatggcttcg acgacggttc gaaatcggtg
     6721 ctcgccaacg gcggcatgaa tggcaagacc ctgacctggg cagacaccca tcaccccggt
     6781 ctaagcgaga ccaacggcga gtacgacggg cagttcctct tcatcaacga caaggccaac
     6841 tcgcgcctgg cggtcatcga cctccgcgac ttcgagacca agcagatcgt caagaacccc
     6901 atcagcatcg acgaccacgg cttctgcgtc acgccggaca ccgagtatgt cctggagggc
     6961 gcgcagtacg cgacgccgct ggggtggaag tacgccccca ttgaggacta tcagaagtcc
     7021 tatggcggga cgctg
//
LOCUS       DATAOZ010000255         5954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3790541, whole genome shotgun sequence.
ACCESSION   DATAOZ010000255 DATAOZ010000000
VERSION     DATAOZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3790541"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VHR64_06715"
     CDS             complement(<1..408)
                     /locus_tag="VHR64_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302557.1"
                     /translation="MHTSVHTPSTELPLRGERLRLSSNTSRQERVSSLIVSAMLAIVL
                     MAIVLIPRFADLDALVTPDEPIWIARSANFYVALADGDLKETYQFSHPGVTVMWLGAI
                     GYRIVDPDLPDRINTHVETADVRDMVIPAGERPV"
     gene            544..3087
                     /locus_tag="VHR64_06720"
     CDS             544..3087
                     /locus_tag="VHR64_06720"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX3302558.1"
                     /translation="MPGSDTWPTAHLDVNVPERVLAFPRADLLPVLVLLIMTGLTAWS
                     HFSLQHRLYDLDTLTFYIPWYSFMGERLRAFDVPGWMPYQFSGAPFAADPQSGWMYMP
                     AMVFFTLFSGAAAQTAFLIFHWLLAGLSMYALARVLGMRPSGALVAAVALEFGTYLEH
                     THTSLLRAQLGAWLPLTLLGIELALRSRRWEGRILAWSLTGFAVTQVLAAWVGQGAYL
                     ALLVAGSYLAYRTLSASTPSLVTWRDRILQLMAGGGTILLVTFGLNAAALIPRLEWNG
                     RSNLAGGDYSDLYQTGGRGWSFARIVDRLTSYDNGLLYWYVGVATITLALVGAVVAFR
                     RFAVPYFVALTGIAIILTLDDTPVHRLFYLLPRFETLHQHQPNRTLVLLTLSLPILAG
                     AAIDRLSTWTRLRVALIVPLVPLILYGLIAVTLPDDLSGIGAPTRNAVIATTLLLFGA
                     VLLGRRRIAWPAFQPATIVTCAAVFLVWWDPTGSAIAHSLRTGQSDPVADGLVTAYTA
                     TTNPGGAGGFLQAQMASQPPFRYFGIDELHWTKKTTRSAWTYSARFNDPAVVEILPGP
                     RAMRLELDGIQGYNPVHLRRYDEYFEAMNGQSQNYHVSEVLRNGASSPLINLLNVRYI
                     VVPNGRDGGWDGAGLVEAGIADREVYADEHVRVLENDDALPRAWIVHEARTVPEGGAL
                     PVLADGSVDPRTVALIEEHVPDLALPRAGANEMVQFLASDSPDRLRFAANLAAPGLVV
                     LSEIYDPGWHAYVDGKRVPVLVTDHILRSVPVSAGSHTIELRYEPATLKIGLAITTAT
                     TITILIVAVIYLLRPRPIRRRTAARMRNMPPSPMASRSPFIPPNHRPTA"
     gene            3138..5579
                     /locus_tag="VHR64_06725"
     CDS             3138..5579
                     /locus_tag="VHR64_06725"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX3302559.1"
                     /translation="MQDSPAAPIAPTASFAAPPAEQLVERATSRQRDLLGLGALLVVV
                     LAVLAVNFWSRYNLYRLDIVTFYIPWYEQLGQRLRDFDIPGWMPYAMSGSPFAGDPQS
                     GWGYLPAMVIFTIAPSLSGYIAFIVFHVILAAAGTYAYARVIGMNPVGAFAAGATFTL
                     GNFMERTACCTIHMQVAVWIPAIFLCIELSRRARTLAGRFGWLVVAGIGTGQMIAGWV
                     GQGAYYGGLAVGVYLLFRTLLSRDEHAGIGERIRALLYSGAVIAIVGGATAAPAVLPR
                     IETVGRSNLADLYEGVVGPDPGWPIRSVPYRIFTDTTSTGRWYLGAVALIAAIIGACL
                     AIRRPRALFFVVYGIGMLSLAVRHSPLVDLANLLPRFHDLHSHSPARIYIVLFLAPAI
                     LAGWLVHTLTDRTWQVKHHVPASVVAIVLGVAALIGTVVSVEHDQGFRVSQDQIVKSA
                     LTIAAVMLALLIRRPWARRLAAVGIILLLLWDPAGELVRYRFDRVHRREQLTAIVDNT
                     LHPNGAAQWLQARAQSGEIFRYFGYDQVLLMNRGQIRTYHVSHDRPEAWAILVNNRGI
                     QFQLDDIQGYNPVQQSIYVELMDSVNGIEQSYHAANVLASGLNSPLLNQLNVQYIVVP
                     RSIPPGRPDLLHLVQSYPTVYEDANSRILENPDALPRAWIVHRADHEDEDEDILTKFS
                     LKLADPSQVVLLTGEPPELERSPDPSAESVEITRYEADEIHLRVTANSTGMVVLSEIW
                     DPGWTATIDGKKAKIYHANAVFRGIVVTPGTHDIVLTYPATNVKRSLLFYIVPLAGLA
                     AIPVLQRRRKGFPAR"
BASE COUNT          977 a         1900 c         1903 g         1174 t
ORIGIN      
        1 caccggtcgc tccccggccg ggatcaccat gtcccggaca tcggcagttt cgacatgcgt
       61 gttgatgcga tccgggagat caggatcgac gatgcgatac ccgatcgcac cgagccacat
      121 gaccgtgacg ccgggatgcg agaactggta ggtctccttc aggtcgccat cggccagcgc
      181 gacataaaaa ttcgcggagc gagcgatcca gatcggctcg tcgggcgtga ccagggcgtc
      241 cagatcggcg aagcggggga tcaacacgat cgccatcagg acgattgcca gcattgcgct
      301 gacgatcagc gatgaaaccc gctcctggcg gctggtattg ctgctgaggc gaaggcgttc
      361 gccacggagc gggagttcag tgctcggagt gtgtacgctc gtgtgcatcg cacaaagttt
      421 cgtcgattga gggccgattt ggcatcccct agactttgcg ggatggaagc agagcacccg
      481 agaggtcaca ccgagatata tcgccctgcg tcgcggccgg ccgtagtccg ggattccacg
      541 gtcgtgcccg ggtccgatac gtggcccact gcccatctgg acgtgaacgt tcccgagcgg
      601 gtgttggcat tcccgcgagc cgatctgctg ccggtgctgg tgcttcttat catgaccggg
      661 ctcacggcct ggagccattt ctcgctgcag caccggctct acgatctcga cacgctgacc
      721 ttctacatcc cctggtacag cttcatgggc gagcgtttac gcgcgttcga cgtgcccggc
      781 tggatgccgt atcagttcag cggtgctccc tttgccgctg acccgcagtc cggctggatg
      841 tacatgccgg cgatggtgtt cttcacgctg ttttccggcg cggcggcgca aactgccttc
      901 ctgatcttcc actggctgct ggccgggctg tcgatgtacg cgctggcgcg ggtgctgggg
      961 atgcgaccgt ccggcgcgct ggtcgcggcg gttgcgctcg agttcgggac ctacctggag
     1021 cacacccaca ccagcttgct ccgcgcccag ctcggcgcct ggcttccgct gaccttgttg
     1081 gggatcgagt tggcactccg atcacggcgc tgggaaggcc ggatactggc ctggtcgctc
     1141 accggatttg ctgtcacgca ggtgcttgca gcctgggtgg ggcaaggtgc gtaccttgcc
     1201 ctgctggtcg ccggctcgta tctggcatac cggaccttgt ccgcatcgac tccgagtctg
     1261 gtgacatggc gggaccggat cctgcagctc atggccggcg gcggcacgat tctgctggtc
     1321 acgttcggcc tcaacgcggc cgcgctgatc cccaggctgg agtggaacgg gcgatcgaac
     1381 cttgccggcg gcgattacag cgacctgtat caaaccggcg gtcgcggctg gagtttcgcg
     1441 cggatcgtcg atcgcctcac gagctacgac aacggcctgc tctactggta tgtcggcgtg
     1501 gcaacgatca cgctcgcgct tgtcggcgcg gtcgtcgcct ttcgccgatt cgcggtcccg
     1561 tacttcgtcg cgctgacggg gatcgcgatc atcctgacgc tcgatgacac gccggtgcat
     1621 cgcctgttct acctgttgcc gaggttcgag acgctccacc agcaccagcc gaaccgcacc
     1681 ctggtcctcc tgaccctgag tttgccgatc ctggccggtg cggcgatcga tcggctctcg
     1741 acctggaccc ggctgcgcgt agctttgatc gtgccgctcg ttccgctgat cctctacggg
     1801 ctgatcgcgg tcacattgcc ggatgacttg agcgggattg gcgcgccgac ccgcaacgcg
     1861 gtgatcgcga ccaccctgct gcttttcggc gcggtattgc ttggcaggag gcgaatcgcc
     1921 tggcccgcat ttcagccggc cacgattgtg acgtgcgccg cggtgttcct ggtctggtgg
     1981 gatccgacgg gatcagccat cgcgcactcg ctccgcacgg ggcaatcgga cccggtcgct
     2041 gacgggctgg tgaccgcgta caccgcgact accaatccgg gcggcgcagg cgggttcctg
     2101 caggcgcaga tggcgagcca gccgccgttc cggtacttcg gtatcgatga gctgcactgg
     2161 accaagaaga cgacgcgctc ggcctggacc tactccgcca ggtttaacga cccggctgtc
     2221 gtcgagatcc tgcccggtcc gcgggcgatg cgactggagc tcgatggcat acaggggtac
     2281 aacccggttc accttcgccg ctacgacgag tatttcgaag cgatgaacgg ccagagccag
     2341 aactatcacg tctcggaggt gctccggaac ggcgcctcat cgccgctgat caacctcctg
     2401 aacgttcgct acatcgtggt gccgaacggc cgtgatggag gatgggacgg cgcagggctg
     2461 gttgaggccg ggatcgccga ccgcgaggtg tatgcggacg agcacgtgcg cgtgctggag
     2521 aatgacgacg ccttgccgcg cgcgtggatc gtccacgaag cgcgcaccgt gccggagggt
     2581 ggcgcgctgc cggtgctggc ggatggatcg gtcgacccgc gcacggttgc gctgatcgag
     2641 gagcacgttc cggatctggc tctacctcgg gccggcgcaa acgagatggt ccagttcctc
     2701 gcctccgata gcccggaccg gcttaggttt gcggccaacc tggctgcgcc cggcctggtc
     2761 gtgctgagcg agatctacga tccgggttgg catgcctatg tcgatggaaa gcgtgtcccg
     2821 gtcctggtca ccgaccacat tctgcggtcg gtaccggtat cagccggcag tcacacgatc
     2881 gagctgcgct acgagccggc aacgctcaag atcggcctcg ccatcacgac cgcaaccaca
     2941 atcacaatcc tgatcgtcgc cgtcatctac ctgctacgcc cacggccgat ccgccgccgt
     3001 acggccgcac gaatgcgaaa catgccgccg tcacccatgg cgtcgcgctc accgttcatc
     3061 ccgcccaatc accggcccac cgcgtgacgt gacgctttgc gagactgctg cgagattttg
     3121 ggcgtatcgt tagcaacatg caggacagtc ccgccgcgcc gatcgctccg actgcatcgt
     3181 ttgccgctcc cccggccgag cagttggttg agcgcgcgac gtcacgtcag cgggatctac
     3241 ttgggcttgg cgcactgttg gttgtcgtgc tggcggtgct tgcggtcaac ttctggagcc
     3301 ggtacaacct ctaccgcctc gatatcgtca ccttctacat cccctggtac gagcagcttg
     3361 ggcagcgtct gcgcgatttc gatatcccgg gctggatgcc ctatgcgatg tccggctcgc
     3421 cattcgccgg cgatccccaa tccggttggg gctacttgcc ggcgatggtc atcttcacga
     3481 tcgcgccatc actgagcggg tacatcgcgt ttatcgtttt ccacgtcatt ctcgcggcgg
     3541 caggtaccta cgcctatgcg cgcgtgatcg ggatgaaccc ggtcggggcc tttgcggccg
     3601 gagcgacctt tacgctcggc aacttcatgg agcgaaccgc ctgctgcacg atccacatgc
     3661 aggtggcggt ctggatcccg gcgatcttcc tctgcatcga gctaagccgc cgcgcgcgga
     3721 ccctggctgg tcgatttggt tggctggtcg tggccgggat cgggaccggg cagatgatcg
     3781 ccggctgggt tgggcagggc gcctactacg gcggactcgc tgtcggcgtg tacctgctct
     3841 tccgaacact cctcagtcgg gatgagcacg ccggaatcgg cgaacgaatc cgggcgttgc
     3901 tgtattcggg tgccgtcatc gcaattgtcg gaggagcgac cgcggcgccg gcggtcctgc
     3961 cgcgaattga gacggtcggc cggtcgaacc tcgccgacct ctatgaaggt gtggttggac
     4021 cggatcccgg ttggcccatc cggtccgttc cgtaccgaat attcaccgac acgacatcga
     4081 cgggtcggtg gtatctcgga gccgtggcgc tgatcgcggc gatcatcggc gcatgcctcg
     4141 cgatccgccg gccgcgcgcg cttttcttcg ttgtctacgg aatcggcatg ctgagtctgg
     4201 ctgtccggca ctcgccgttg gtggacctcg cgaacctgct gccgagattc catgatctgc
     4261 acagtcactc gcccgcccgg atctacatcg tgctcttcct ggcgccggca atcctggccg
     4321 gctggctggt ccacacgctt acggatcgaa cctggcaggt gaagcaccac gtgcccgcgt
     4381 cggtggtggc gatcgtcctt ggcgtggctg cgctcattgg cactgtggtt tcagtcgagc
     4441 acgatcaggg gttccgggtc agccaggacc agatcgtcaa atcggccctc acgattgccg
     4501 cagtcatgct ggcgttgctg atccgccgac cgtgggcgcg gcgactggcg gcggtaggaa
     4561 tcatcctgct tctgctctgg gatccggcgg gcgagttggt ccgttaccgg ttcgaccgtg
     4621 ttcaccggag ggagcagctg acggcaatcg tcgacaacac cttgcatcca aacggcgccg
     4681 cccagtggct gcaggccagg gctcagtcgg gcgagatatt ccgctacttt gggtacgacc
     4741 aggtcctgct gatgaatcgc ggccaaattc ggacttatca cgtctcgcac gatcggccgg
     4801 aagcgtgggc gatcctcgtc aacaaccgcg gcatccagtt ccagctcgac gatatccagg
     4861 gctacaaccc ggtccagcag agcatctatg tggagctgat ggattcagtt aacgggatcg
     4921 agcagagcta ccacgctgcc aatgtgctgg cgagcggtct caattccccg ttgttgaacc
     4981 agctcaatgt gcagtacatc gtagttccgc gctcaatccc gcccggccgg ccggatctgc
     5041 tgcacctcgt ccagtcatat ccgacagtct acgaagatgc caactcgcga atcctcgaga
     5101 atccggacgc attgccacgg gcgtggatcg tccaccgggc cgaccatgaa gacgaggacg
     5161 aggacatcct gacgaagttc tcgctcaagc tggcagaccc gtcgcaggtc gtcttgctca
     5221 cgggcgagcc gccggagctg gagcgatcgc ctgacccgtc agcggagtcg gtcgagatca
     5281 cgcggtatga ggcggacgag atccacctgc gcgtcacggc caactcgacc ggcatggtcg
     5341 tgctgagtga gatctgggac ccgggctgga ccgcaacgat cgatggcaag aaggcgaaga
     5401 tctaccacgc aaatgccgtc ttccgcggca tcgtcgtcac tcccggcacc cacgacatcg
     5461 ttctcacata ccctgccacc aacgtaaaac gcagcctgct gttttacatc gtacctttgg
     5521 cgggcctcgc tgccattccg gtattgcaac ggcgccggaa gggcttcccc gccagatagg
     5581 ccgcacaggc cagacggtcc ctgaccgtca cagggactcc catgtgcggg cgtgccaaat
     5641 cgatggccgg tcgagtacag ccgtatttcg gggatgccgc ccgcaaggtg attctttcgg
     5701 atggaaagtg acagcagact gcgagcattc gcccgctcag atacccggat ggctcgaacc
     5761 agtggggtat caccgagcgg acggccgtta aaggcggcaa tgttcgttcg ctgggcagcc
     5821 ggaggaaatc gcaatggttc cgtacgtcat tgcttgggtg ttagatcaga tcgagcgtga
     5881 ggaagagggc cagggtttgg tcgagtacgc actcatcctg gtgctggtct cgatcgccgc
     5941 gatcgtcgcg ctga
//
LOCUS       DATAOZ010000256         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4053696, whole genome shotgun sequence.
ACCESSION   DATAOZ010000256 DATAOZ010000000
VERSION     DATAOZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4053696"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VHR64_06730"
     CDS             complement(<1..522)
                     /locus_tag="VHR64_06730"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3302560.1"
                     /translation="MSNRPPGVRLLLFAAFALLATVSLGNAVSAQKSGFDPAKYTLQT
                     TKVVDGLDAPLDMVDPNDGSGRMFIVEKGGRVLILKDGQVQPDPFLDISAQVSTGSEQ
                     GLLSIALHPDFKTNGTLFIDYTDTDGNSQIERWNVSKDNPDLADPASAETILSVEQPY
                     ANHNGGLLLFGPDG"
     gene            767..1444
                     /gene="lepB"
                     /locus_tag="VHR64_06735"
     CDS             767..1444
                     /gene="lepB"
                     /locus_tag="VHR64_06735"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX3302561.1"
                     /translation="MVEPIEQQDETPQATDAAEKVSLWPELFKTLMMALIIFLTARLF
                     VLPYQVDGRSMAPNLENRDRVLVNRAVYMHLNLDLLIGWIPGVDTDDTDYYPFHSPER
                     GDIVVLYPPDSSPEPYIKRTIAVAGDRVDIHDGLVFVNGERLVEPYIEGAVTECDQTT
                     YCDGFVVPPGMIYVLGDNRQHSFDSRAFGPVALDNVIGKAWFANWPADRIGPLPHYSY
                     DDATPKQ"
     gene            complement(1486..2247)
                     /locus_tag="VHR64_06740"
     CDS             complement(1486..2247)
                     /locus_tag="VHR64_06740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3302562.1"
                     /translation="MLAGKVAIVTGAGSGLGQATAIALAGEGAEVFITELPDRLDRAG
                     ETLSTIVHAGGTGHVRTLDVRSLDSISRCVESVVEQSGRIDILVNNAGVNIRKPALEV
                     TESDWDTVLDVNLKGAFFMAQAAGRQMHAQNPSGGSIINIASIMGLGGYWDRVAYCAS
                     KAAMINMGRVLALEWGPQNIRVNAVCPNFVITPLTEPLFAANPEFTEDVMRRTPIGRL
                     PTPEEIAAAVVYLASPGAAAVTGQTLTVDGGWTAH"
     gene            2625..3716
                     /locus_tag="VHR64_06745"
     CDS             2625..3716
                     /locus_tag="VHR64_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3302563.1"
                     /translation="MGQLGKGLNRKEVRSLVNNVTRRGFLGGGAAFTALAAMGGLRPG
                     VTAAEAIHNLGILPDDLPATKYKAATIEVGAASTWVAHGIETSKFFGKLLGVEVEPFD
                     GQFDPANQLSAFQTVSQGTYDFVCVHPAASDALIDGSDAVIAKGWPLVVMDTRLVQDP
                     EAFKNYGHLTFIEPDNIYMGFTVANELFKAIGGEGEVIHTQGQLAHTGAQGRAQGFKL
                     ALEAYPNIKVVDEQPGNWQVEVVASLWQDLLQRFPNVKGGFFHSDDMALAARAVIDAA
                     GKRDQVKIVGVDGLKNACEAIIAGDMTASVINPSGRIHGGSIWAGYLTVSKTDMSTAP
                     IPKFIRADGGPIVVDNAPGYIWLGDNLQY"
BASE COUNT          696 a         1174 c         1259 g          715 t
ORIGIN      
        1 gccgtccggg ccgaagagga gcagaccccc gttgtggttc gcgtagggct gctcgaccga
       61 caggatcgtt tcggcgctgg ccggatcggc gagatccggg ttgtccttgc tgacgttcca
      121 gcgctcgatc tgggagttcc cgtcggtgtc ggtgtaatcg atgaagaggg tgccgttcgt
      181 cttgaagtcc gggtgcagcg cgatgctgag caatccttgc tccgaaccag tactgacctg
      241 cgccgagatg tcgaggaatg gatccggctg gacctgcccg tccttcagga tcagcacacg
      301 gccgcccttc tctacgatga acatccgccc gctgccgtcg ttcgggtcga ccatgtcgag
      361 cggcgcgtcc agaccgtcga cgacctttgt cgtctgcagg gtgtatttgg cgggatcgaa
      421 gccgctcttt tgcgcggaga cagcattgcc gagcgacacg gttgccaaca gcgcgaaggc
      481 ggcgaagagg agtaatcgga cacctggcgg gcgattcgac atcgtgatca gcgctccttt
      541 gcagaaagca gtcggaccct tgcggtgtgg cgggaccttg ccggagacgg gacagatggg
      601 caagataggg ggatgaagtg gctgtccaat gctgtcattc tgcccatcgg gccgattcct
      661 gccaagttcc ggcgcgacgt accggcaatc cggtggctgt tgtccgtcgt tgagttgagg
      721 caggatggtg ggctgaaacc ggtccaaacg aagcgagagt cagtgcgtgg ttgagccgat
      781 cgaacagcag gatgagacac cgcaagcgac tgatgccgcg gagaaggttt cgctctggcc
      841 agagttgttc aagacgctga tgatggcgct gatcatcttc ctgacggcgc ggctcttcgt
      901 cctgccgtac caggtggatg gccgcagcat ggcgcccaat ctcgagaacc gggatcgggt
      961 actggtcaac cgcgccgtct acatgcacct caacctcgac ctgctcatcg gctggatacc
     1021 cggtgtcgac accgatgaca cggactacta cccgttccac tcgccggagc ggggcgacat
     1081 cgtcgtgctc tacccgccgg actcttcgcc ggagccatac atcaagcgga cgatcgcggt
     1141 cgcgggcgac agagtcgata tccacgacgg gctggtgttc gtcaacggcg aacggcttgt
     1201 tgagccgtac atcgagggcg cggtcaccga atgcgatcag acgacctact gcgatgggtt
     1261 cgtcgtgccg ccgggcatga tttacgtgct cggggacaat cggcagcaca gtttcgattc
     1321 gcgcgcattc ggcccggttg ccttggacaa cgtgatcggg aaggcatggt tcgcgaactg
     1381 gccggcggac cgaatcggcc ccctcccgca ctacagctac gacgacgcca ccccaaagca
     1441 gtgatcccga tccccatccc gcactacgtt ggctgatgta tcggcttagt gcgcggtcca
     1501 accgccgtcg acggtcaggg tttggccggt gactgcggcg gcgcctgggc ttgcgaggta
     1561 gacgacggcc gcggcgattt cctccggcgt cggcagcctg ccgatcggcg tccgccgcat
     1621 cacgtcctcg gtgaactccg gattggcggc gaagagcggc tcggttagcg gggtgatcac
     1681 gaagttcggg cagacggcat tgacccggat gttctgcggt ccccactcga gcgcgaggac
     1741 gcggcccatg ttgatcatcg ccgccttgct ggcgcagtag gcgacgcgat cccagtagcc
     1801 gccgaggccc atgatgcttg caatgttgat gatcgagccg ccggacgggt tctgcgcatg
     1861 catctggcga ccggccgcct gggccatgaa gaaggcgccc ttcaggttga cgtcgaggac
     1921 cgtgtcccag tcagattccg tcacctcgag ggccggcttg cggatgttga cgccggcgtt
     1981 gttcaccagg atatcgatcc ggccggactg ctcgacgacg ctctccacgc agcgggaaat
     2041 cgagtcgagc gagcgcacat cgagtgtgcg gacgtgtccg gtgccgccgg cgtggacgat
     2101 agtggaaagg gtctcgccgg cgcgatcgag gcgatcgggc aactcagtga taaagacctc
     2161 agccccttcg ccggcgagcg cgatcgccgt cgcctgcccc aacccggagc ccgccccggt
     2221 cacgatcgcc actttccccg ccagcatggt tcccaatgtg gtttctcctc tgtccggtcg
     2281 ggattgtaga gggagtcgag gaggcgagca gtgaggcgcg gagacgggaa ggcgcggagg
     2341 cgcgatgttc gggcgagcag tcggaaaacc gaaaaccaga gacgaaagac cagagacgaa
     2401 aaacgcattt ccgccgtatg ccgttgaccg ctctccgggg ttgtgctagt ttgccttacg
     2461 tttcgtacac aagagcgcgg gagattcggg gcggttcgcc cggtactcgt tccacctacc
     2521 ttgggcctcg cgaacgcctc cctcatacgg cgttccctgg ttatagcggc agtcgctacg
     2581 ccttgttgcg gctgtggacc gacaaccgac ggaggaagat cgcgatgggt cagcttggga
     2641 agggattgaa ccggaaagag gttcgctcgc tcgtaaacaa tgtcacgcgt cgcggctttc
     2701 ttggtggtgg cgcggcattc accgcactcg cggccatggg tggactgcgt ccgggagtga
     2761 cggcagccga ggctatccac aacctcggta tcctgcccga cgatcttccg gccacgaagt
     2821 acaaggccgc cacgatcgaa gtcggtgcgg cgtcgacctg ggttgcgcac ggaatcgaga
     2881 catcgaagtt cttcggcaag ctgctcggtg tcgaggttga gccgttcgac ggccagttcg
     2941 acccggcaaa ccagctctct gccttccaga cggtctcgca gggcacgtac gacttcgtct
     3001 gcgtccaccc ggccgcgtcc gacgcgctga tcgacggctc agacgccgtc atcgccaaag
     3061 gttggccatt ggtcgtcatg gacacgcgcc tggtgcagga tccggaagcg ttcaagaact
     3121 acggccatct gaccttcatt gagccggaca acatctacat gggcttcacg gtcgccaatg
     3181 agctcttcaa ggcgatcggc ggcgagggcg aggtcatcca cacccagggt caacttgccc
     3241 acaccggcgc gcagggtcgc gcccagggtt tcaagcttgc gcttgaggcg tacccgaaca
     3301 tcaaagtcgt agacgagcag ccgggcaatt ggcaggtcga ggtcgttgcc tcgctctggc
     3361 aggaccttct gcagcgcttc ccgaacgtca agggtggctt cttccactcg gacgacatgg
     3421 cacttgcggc tcgcgccgtg atcgatgcgg ccggcaagcg ggatcaggtc aagatcgtcg
     3481 gcgtggacgg tctcaagaat gcctgcgaag cgatcattgc cggtgacatg acggcatcgg
     3541 tgatcaaccc atctggccgc atccacggcg gttcgatctg ggccgggtac ctgaccgtct
     3601 ccaagacgga catgtcgacc gcgccgattc cgaagttcat ccgcgctgac ggcggaccga
     3661 tcgttgtcga taacgcgccc ggttacatct ggctcggcga caacctgcag tactagaggg
     3721 cccgaccgga gccggcggga acgaggggtc ggatcgatgg cggaaacacc cggaacgctc
     3781 aacgtggagc aggatgtgtc atcgtcatcc ctctcttctc cggccaccgg ctacctaccg
     3841 gtcg
//
LOCUS       DATAOZ010000257         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4843521, whole genome shotgun sequence.
ACCESSION   DATAOZ010000257 DATAOZ010000000
VERSION     DATAOZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4843521"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..882)
                     /locus_tag="VHR64_06750"
     CDS             complement(184..882)
                     /locus_tag="VHR64_06750"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX3302564.1"
                     /translation="MSQAPMQAGAQATPVAQTTSPGTISVTGVGEVIVTPDTANIQIG
                     VQVFAEELSAAQQKSNQQTQAIIDTLKEAGIKEKDIQTSNYSVSIRQQYDNQGIATAV
                     LGYDIYNTLSVTVRDLDKLGEILDKVVAAGANQIYGIYFYTQDLTAATKQARAAAVKD
                     AQERAGQLADAAGVSVGRIINISEGYSGAPQPLQYGQGQAAADMKGGAGAVPIQTGSQ
                     SVQISVSMTFEIAP"
     gene            complement(1055..2053)
                     /locus_tag="VHR64_06755"
     CDS             complement(1055..2053)
                     /locus_tag="VHR64_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX3302565.1"
                     /translation="MGASDRPRSEDHRPRDDRHLSEDVLNRYIDGDLDSTERAQAEVH
                     LSQCAECRSELADVRMLASLLHELPQVEVPRSFQLGPEHARPASWWDRLASLLLPMLP
                     AMRATTVALVLVLGGLTAYRVVEDQPSSGPVSELAAPVATTTAVGGGQFAGTSTPANT
                     ETPVQIAAQKAPPPTDSASRNSGNNAAADESGAGGEAPAQSEPSDAANSAGSQPSNST
                     ELQLPGQAQPAPTESTEESAASSASDTTIMVAAEPASPTASPTATASPSPSPTASPTA
                     TPSPTVTPSPTPTIAPASTGTSDRAWLGWVQAILAAGILILGGLMIGLTRFRKPVP"
     gene            complement(2087..2785)
                     /locus_tag="VHR64_06760"
     CDS             complement(2087..2785)
                     /locus_tag="VHR64_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3302566.1"
                     /translation="MFRRRTQPCATTTEEPEPPELDLEARLDERLIQNAQQGDLPSFN
                     TLVTRHQRSVYNVCLRMLRDSALAEDAAQDTFIKAWTSIGTFRGGLVRPWLLRIATNR
                     CYDILRSQGRRPASSLDAELVESEPQWTSQTAIAEHPEGHATRRELSAFLERALGELP
                     DDQRLAIILSDVQGHSYDEIAQITEVAVGTVKSRIFRARARLRELVLSETNAREHLER
                     YQRLVNSANQESAG"
     gene            complement(2854..>3173)
                     /locus_tag="VHR64_06765"
     CDS             complement(2854..>3173)
                     /locus_tag="VHR64_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3302567.1"
                     /translation="GVLLVNTARGPIVNLADLNDALESGHVAGAALDVFDPEPYPLDQ
                     ALAAQPNLIMTPHAAYYSEESVEIRRRETLIAVLDVLAGREPVVVANPAVLPNLDLKP
                     RCA"
BASE COUNT          484 a         1035 c         1082 g          572 t
ORIGIN      
        1 ggaccgatgc cggccgggcg cgaatcacga acggcgcggc gaccaacccg gcgccgacga
       61 gcatgccgcg ccgcgtgatg ccggattgcg gcatggttcg tggtcgcatg gcgcgagttt
      121 acccgaaacg aaacagggga ccggctgccc ggtcccctgt cgcaggttcg attcgtggtg
      181 ctgttatggg gcgatctcga aggtcatcga gaccgagatc tgaaccgact gcgagcccgt
      241 ctggatcggc accgcaccgg cgccgccctt catgtccgcc gcggcctgcc cctggccata
      301 ctggagcggc tgcggtgctc cgctgtagcc ctcgctgatg ttgatgatcc ggccgaccga
      361 gactccggcg gcatccgcga gctgacccgc ccgctcctgc gcatccttga ccgccgccgc
      421 gcgcgcctgc ttcgtggccg ccgtcaggtc ctgcgtatag aagtagatgc cgtagatctg
      481 gttcgcgccg gccgcgacca ccttgtcgag gatttcgccg agcttgtcga ggtcgcgcac
      541 ggtcacgctc agcgtgttgt agatgtcata cccaaggacc gcggtcgcga tgccctggtt
      601 gtcgtactgc tggcggatcg agaccgagta gttgctggtc tggatatcct tttccttgat
      661 cccggcttcc ttgagcgtgt cgatgatggc ctgggtctgc tggttcgact tctgctgtgc
      721 cgccgagagc tcttcggcga agacctggac gccgatctgg atgttggcgg tatccggcgt
      781 gacgattacc tcgccgacac cggtgaccga gatcgtgccg gggctggtcg tctgcgcgac
      841 cggcgtagcc tgcgcgccgg cctgcatcgg ggcctgggac atgaccatca cccccaacag
      901 cgcgagtgga acgaaaaact gaccgaacct gcgtttgacc gacgatcttt ccactggatt
      961 ctcccttctg tcgcaacctc catagttgcc ttcaccacca caacgccggg tcgatctgga
     1021 aagttccacg cggtcgagtc agggccgtcc ggctctatgg taccggcttg cggaagcgcg
     1081 tgagtccgat catgaggcca cccagaatca gaattccggc ggcaagaatc gcctgaaccc
     1141 aaccaagcca ggcccggtcg ctggtcccgg tcgatgccgg ggcgatggtc ggcgtcgggc
     1201 tcggcgtcac cgtcgggctc ggggtcgccg tggggctggc ggtcggcgac ggagaaggtg
     1261 atgccgtggc ggtcggactt gccgtcggcg acgcgggctc cgccgcaacc atgatcgtgg
     1321 tatccgatgc ggagctggcc gcggactctt cggtcgactc cgtcggagcc ggctgcgcct
     1381 gccccggcag ttgcagctcg gtggagttgc tcggttggct tccggcggag ttcgctgcgt
     1441 cactcggctc ggattgggcc ggcgcctccc ctcccgcccc cgattcatcg gctgccgcat
     1501 tgttgccgga attgcgcgac gccgaatcgg tcggcggcgg cgccttctgg gccgcaatct
     1561 gaaccggcgt ctcagtattg gcaggggtgc ttgtcccggc gaattgcccg ccgccaactg
     1621 cagtggtcgt cgccaccggc gccgccagct ctgagactgg tccggacgac ggctgatctt
     1681 cgacgacccg gtacgcggtg aggccgccga gcaccagcac gagcgcaacg gtcgtggccc
     1741 gcatcgccgg aagcatcggc aggaggagag acgcgagacg atcccaccac gaggccggcc
     1801 gcgcatgttc cggaccgagc tggaacgagc gggggacctc gacttgcggc agctcgtgga
     1861 gaagcgaggc gagcatccgg acgtcggcca gctcgcttcg acattccgcg cactgcgaga
     1921 ggtgcacctc ggcctgcgct cgctcggtcg agtcgaggtc gccgtcgatg tatcgattga
     1981 gcacgtcctc gctcagatgg cgatcatcgc gcgggcgatg gtcctccgag cgcggacggt
     2041 cgctagcgcc catggccgca tccctggatc gcgaacagtg cccggctcat ccggccgact
     2101 cctggttcgc gctgttaaca agacgttgat atcgctcgag atgttcccgg gcgttggttt
     2161 cggacaacac cagctcgcgc agcctggccc gcgcccgaaa gatgcgcgat ttgaccgtgc
     2221 cgaccgcgac ctcggttatc tgggcaatct cgtcgtagga gtgaccttgc acgtcggaca
     2281 ggatgatcgc caggcgctgg tcatcgggca gctcgccgag ggcacgctcg agaaaggccg
     2341 aaagctcgcg ccgggtggca tgaccttccg ggtgctcggc gattgcggtc tggctggtcc
     2401 attgcggttc cgactcaacg agctcggcat cgagcgagct cgccggccgg cgaccctggc
     2461 tgcgaaggat gtcgtagcag cggttggtcg cgattcggag cagccagggc cggaccagtc
     2521 cgccccggaa cgtgccgatc gaggtccacg ccttgatgaa ggtgtcctgc gcggcgtcct
     2581 cggccagcgc gctgtcacgc agcatccgca ggcagacgtt gtaaaccgat cgttgatgcc
     2641 gggtaaccag cgtgttgaac gacggcaggt cgccctgctg cgcgttctgg atcagacgct
     2701 cgtccagccg ggcctcgagg tcgagctcgg gcggttcggg ctcttcggtt gtcgtcgcgc
     2761 agggctgcgt tcggcgtcgg aacatgtccg gtgtcgatcc tgtctggtcg cggttctacc
     2821 tatctgagac gcgcgagccc ggcaatcggt gtgtcacgcg cagcgcggct tcaggtcaag
     2881 attcggcagc acagccggat tggccacgac gaccggctcg cgaccggcca ggacgtccag
     2941 cacggcgatc agggtctcgc gccggcgaat ctcgaccgac tcctcggagt agtaggcggc
     3001 gtgcggcgtc atgatcaggt tcggctgagc agcgagcgcc tgatcgagtg gatacggctc
     3061 cggatcgaag acatcgagcg cggccccagc gacatgcccg ctctcgagcg cgtcgttcag
     3121 gtcggcgaga ttcacgatcg ggccacgggc cgtattgacc agcagcacac ccg
//
LOCUS       DATAOZ010000258         5193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1423459, whole genome shotgun sequence.
ACCESSION   DATAOZ010000258 DATAOZ010000000
VERSION     DATAOZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423459"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1616
                     /locus_tag="VHR64_06770"
     CDS             <1..1616
                     /locus_tag="VHR64_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX3302568.1"
                     /translation="LRCRQQLERGTGMKLAEIRDLDGPNLFLLRPAIKVELILEDGET
                     ESKLADRLASGSVDLDAATRQFFTDVYDQHEQPTPQFVWRHMDEPGHVSLAFAWVRRR
                     FAMAVAQLIVDTINGVVDDNIDNHLNHLLNSDARDEFPECVLDADRSAIAISVTGTNG
                     KTTTTRLIAHLMQTAGYRTGWNSSSGIYIDGEEIEAGDYSGPSGARRVLEDPSVQAAV
                     LETARGGILLRGLGYEHNDVSVFTNVSADHLDLQGVRTLETLAEVKAVVCKVTRPQGV
                     AVVNADDPLVMVATADIVAQRTLFTQRSDNDAVASHLASEGRAILADRDQIVVRKGDQ
                     IVGTFPLAEIPMTFGGRARHMVENAMAGIGAALGAGLGVAQIRAGLLSFRNDSSGNLG
                     RMNIYTCNGITIVLDFAHNEAGLAFLVDFGRSEIGSDGRLITIIGTAGDRTEHSLREI
                     GHIAAERSDIVIAKGTHKYLRGRTLDDLMALYRAGANQCPDTEYHESESEVSALEDAL
                     QIARSGDVIVLMAQEYLPELTARLKAIST"
     gene            complement(1676..4324)
                     /gene="cphA"
                     /locus_tag="VHR64_06775"
     CDS             complement(1676..4324)
                     /gene="cphA"
                     /locus_tag="VHR64_06775"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX3302569.1"
                     /translation="MIEVRKTTVYRGPNVWARMPVILMILDIGELEEQPTNKIPGFYE
                     RLTEYMPSLYDHGCSLGRPGGFLQRMREGTWMGHVLEHVALEFQNLSGAEVSRGKTRS
                     TGERGVYNVVYEHVQEDVGLAAGRLGVRLLNHLIYNAEPDFNFQAELEENVIRVAERL
                     AYGPSTGSIVSEAERRNIPVLRLDPKRSLVQLGHGIHQKRVWATVTSRTSNISVDVAA
                     NKELTNRLLQEVGIPVPRGQVVRNVDDAVKAANRIGYPVVIKPLDGNHGRGVCINLTD
                     DEEVREFYRIAEAESRAGIVQVESFITGRDYRILVVNGQIVAVAERVPAHVVGDGVHT
                     VEQLIDITNSDPRRGVGHEKILTRISIDSQTMETLERQGLTLTDVPALDQVVKLKQTG
                     NISTGGTSIDRTDDIHPDNIEIARQAAMVVGLDIAGIDFVTSDIAESVRQSGGAIVEV
                     NAGPGFRMHTNPTEGHPRHVGRAVVDMLFPPGATSRVPIVAVTGTNGKTTTVRMISHI
                     MKTAGRRVGMTTTDGIYIDGTQILAGDMSGPTSAQMVLKNPTIDYAVLETARGGILRS
                     GLGFDRCDIAVVTNIASDHLGLGGIESIDDLARVKAVVPQAVFRDGVSVLNADNEWTV
                     DMARTARGEIIFFSMNEDSPVLREHLRQRGRAVVLRPTRHGDMITIIEHRRETSLILA
                     SEIPATFGGRLRVNIANAMAAASAAVGGDVQFEHIRQGLRSFSSSYFQTPGRFNFLDV
                     DGRRVVMDYCHNVAGLESMADFVKRLGAPHAVGMIAMPGDRLDTDIEAFGDLAARTFD
                     RIVIREDVNRRGRADGEIAEMLRQSARNAGMNDDRICIVLEEIEAVNAAIEMAERDDL
                     VVLLVDKPAAAWRELEKRVSLRGELV"
     gene            complement(4356..5168)
                     /locus_tag="VHR64_06780"
     CDS             complement(4356..5168)
                     /locus_tag="VHR64_06780"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX3302570.1"
                     /translation="MRYNGADGHPGPIMAIGGAEDKFRERTILSTFMALSGMADARIV
                     IIPTASSIETAGERYKAIFLEFGARSAHIAFVHDRAHANEPEWNTVFANATGIFITGG
                     NQLRLSTRLGGTLVEQEIKRAWNGGATVAGTSAGASILSSHMVAFGASGPTPRQRMAQ
                     MVGGFGLVDQAVIDQHFRQRDRTGRLMSLVAGSPGLLGVGVDEDTAAVFGSEGTIDVV
                     GRHSVTIIDGSRIYTDYPEVRGFGEITVSGAVIHSLTAGRRFDTRNRRMMQL"
BASE COUNT          955 a         1650 c         1616 g          972 t
ORIGIN      
        1 gtttgcgctg caggcagcaa ttggagcgcg ggacagggat gaagctagcc gaaatccgcg
       61 acctcgacgg tcccaatctg ttcctgctcc ggcccgctat caaggtcgag ctcatactcg
      121 aggacggtga gaccgaatcg aagctcgccg atcgattggc gtccggatcc gtcgacctgg
      181 atgcggccac gcggcaattc ttcaccgacg tatatgacca acatgagcag ccaaccccgc
      241 agttcgtctg gcggcatatg gacgagcctg gtcacgtttc gcttgcgttc gcgtgggttc
      301 ggcgccgctt cgcgatggca gttgcgcagc ttatcgtcga cacgatcaat ggcgttgttg
      361 acgacaacat cgacaaccat ttgaatcatc tgttgaactc ggatgcccgg gacgaatttc
      421 cggagtgcgt tcttgacgca gacaggtcgg caatagcgat ctcggtcacc ggaaccaacg
      481 gcaagacgac gaccacccgc ctgatcgccc acctgatgca gacggccggc tatcgcaccg
      541 ggtggaactc gtcgagcggc atctacatcg acggcgagga gatcgaggca ggcgactact
      601 cagggccatc cggagctcga cgagtcctcg aggatccctc tgtgcaggcg gccgttctcg
      661 agacggcgcg gggcggcatc ctgctacgcg gcctgggata cgagcacaac gatgtttctg
      721 tctttaccaa cgtcagcgcg gaccacctcg acctgcaggg cgtacgaacg ttggagacac
      781 tcgctgaggt gaaagccgtc gtctgtaagg tgacgcggcc gcaaggcgtg gccgtggtga
      841 atgccgacga tccccttgtc atggtcgcca ctgccgatat cgtggcgcag cgcaccctgt
      901 tcacccagag gtccgacaac gacgccgtcg catctcacct ggcgagcgag ggtcgagcga
      961 tcctggccga cagggatcag atcgtggttc gaaaaggcga tcagatcgtc ggcacctttc
     1021 cgctggccga gattccgatg acgttcggtg gccgggcccg acacatggtc gagaatgcga
     1081 tggctggtat cggggcggcg ctcggtgcgg gtcttggggt cgcgcagata cgcgccgggc
     1141 ttctttcgtt ccggaacgac tcgagtggaa atctcggccg gatgaacatc tacacgtgca
     1201 acggcatcac gatcgtgctg gacttcgccc acaacgaggc gggtctggcg ttcctggtgg
     1261 atttcggtcg atcggagatc ggttctgatg gccggctgat cacgatcatc ggcacggccg
     1321 gcgatcgcac cgagcactcg ctgcgagaga ttggccatat cgccgccgag cgatcggata
     1381 tcgtgatcgc caagggcacg cacaagtacc tgcgcggcag gacgctggac gatctgatgg
     1441 cgctctaccg cgccggcgcg aaccagtgcc cggacaccga gtaccacgag tccgaaagcg
     1501 aagtcagcgc gctcgaagat gcgcttcaga tcgctcgatc aggcgacgtc atcgtcctga
     1561 tggcgcagga atacctgccg gagcttaccg ctcgcctgaa agcgatctcg acctagttcg
     1621 acgcacgtac ttccgggcgg gtcgctcgga actagtgtga acaatcggac tcgctctaca
     1681 ccagctctcc gcgaagcgag acgcgcttct cgagctcgcg ccatgctgcg gccggtttgt
     1741 cgaccagcaa tacgaccagg tcgtcgcgct cggccatctc gatcgccgcg ttcactgcct
     1801 cgatctcttc gagcacgatg cagatccggt cgtcgttcat gccggcgttg cgcgcggact
     1861 ggcgaagcat ctcggcgatc tcgccatcgg cccggccgcg acggttgaca tcctcccgga
     1921 tgacgatccg gtcgaacgtc cgcgccgcaa ggtcgccaaa cgcttcaata tcggtatcga
     1981 gccgatctcc cggcatcgcg atcatgccga ctgcatgcgg cgcgccgagt cgtttcacga
     2041 agtcggccat cgattcaagc ccggccacgt tgtggcagta gtccatcacc acgcgccggc
     2101 catcgacatc gaggaaattg aagcggccgg gcgtctggaa gtagctggac gagaagctgc
     2161 gcaacccctg ccgaatgtgc tcgaactgga catcgccgcc gaccgccgcc gaagccgccg
     2221 ccatcgcgtt cgcgatgttg accctcaatc gcccgccgaa tgtggccgga atctcgctgg
     2281 ccaggatcag gctcgtctcg cggcgatgct cgatgatcgt gatcatgtcg ccgtgccgtg
     2341 tcggtcgcaa cacgacagcc cgaccgcgtt gccgcaagtg ctcgcgcaga actggcgagt
     2401 cttcattcat gctgaagaag atgatctcgc cccgcgctgt gcgcgccatg tcgacggtcc
     2461 actcgttgtc ggcatttagc accgagacgc catcgcggaa gacggcctgc ggcacgaccg
     2521 ccttgacccg ggccaggtca tcaatcgact cgatgccgcc aagaccgagg tggtccgagg
     2581 cgatattcgt gaccacggcg atatcgcagc gatcgaaacc gaggccggag cgaaggattc
     2641 cgccacgggc ggtctcgagg accgcgtagt cgattgtcgg gttcttgagg accatctggg
     2701 cgctggtcgg cccgctcata tcgccggcaa ggatctgcgt gccgtcgata tagattccgt
     2761 cggtcgtcgt catgccgacg cgccgaccgg ctgtcttcat gatgtgcgag atcattcgca
     2821 ccgtcgtagt cttgccattg gtgccggtga ccgcaacgat cggcacccgg gatgtcgcgc
     2881 cgggcgggaa aagcatgtcg acgaccgccc ggccgacatg gcgcgggtga ccctcggtcg
     2941 ggttcgtgtg catccggaac ccgggtccgg cgttgacctc gacgatcgcg ccgccactct
     3001 ggcgcaccga ctcggcgatg tcggaggtaa cgaagtcgat accggcgata tcgagcccga
     3061 cgaccatggc agcctgccga gcgatctcga tgttgtccgg gtggatgtcg tcagtccggt
     3121 caatcgacgt accaccagtg cttatgttgc cggtttgttt aagcttgacg acttgatcga
     3181 gggccggcac gtctgtcagc gtcaggcctt ggcgttccaa cgtttccatc gtctggctat
     3241 cgatcgagat ccgcgtcagg atcttctcgt gaccaacgcc acgacgcgga tccgagttag
     3301 tgatgtcgat cagctgctcc accgtatgaa caccgtcgcc gacgacgtgc gcagggacgc
     3361 gctcggccac cgctacgatc tgcccattta cgaccaggat ccggtaatcg cgaccggtga
     3421 taaagctctc aacctggacg atgccggccc ggctctcggc ctctgcgatg cggtagaact
     3481 cgcgaacttc ttcgtcatcc gtgagattga tgcagacacc gcgcccgtgg ttcccatcga
     3541 gcggcttgat cacaaccggg taaccgatcc ggttcgccgc cttgaccgcg tcgtcgacgt
     3601 ttctcacgac ctgcccgcgc ggaaccggga tcccgacctc ctggagcagg cggttcgtca
     3661 gctccttgtt ggccgcgaca tcgaccgaga tgttcgaggt ccgtgacgtc acagtcgccc
     3721 agacgcgctt ctggtgaatg ccgtggccaa gttgcaccag cgagcgcttc gggtcgagtc
     3781 gaagcaccgg gatattgcgt cgctcggcct cggagacgat ggaaccggtc gacggcccat
     3841 aggcgagccg ctccgcgacc cggatcacgt tctcttcgag ctcggcctgg aagttgaagt
     3901 ccggctcggc gttgtagatc aggtgattaa gaaggcgtac gccgaggcga ccggccgcca
     3961 ggccgacgtc ttcctgcacg tgctcgtaga cgacgttgta gacgccgcgc tcgccggtac
     4021 tgcgcgtctt gccgcgcgaa acctcggcgc cggacagatt ctggaactcg agcgcgacat
     4081 gctcgagcac atgacccatc caagtgcctt cgcgcatgcg ctggaggaat ccgcctggtc
     4141 ggcccagcga gcagccatgg tcgtaaagcg acggcatgta ctcggtcaac cgctcgtaga
     4201 agccgggaat cttgttggtg ggctgctcct cgagctctcc gatgtcgagg atcatcagga
     4261 tcacgggcat gcgcgcccag acattgggac ctcggtaaac agtggtcttc cgaacttcga
     4321 tcattggtcc cttgcccctg aatgtcgcct gatcgctaca actgcatcat ccgccgattt
     4381 ctggtgtcga aacggcgccc cgcggtcagc gaatggatca ccgccccact gacggtgatt
     4441 tcgccgaagc ctctgacctc cggatagtcg gtatagatgc gtgacccatc gataatcgta
     4501 acggaatgac gaccgactac gtcaatagtc ccttcagacc cgaagaccgc cgccgtgtcc
     4561 tcatcaacgc cgacgccgag gagcccgggg ctgccggcaa ccagcgacat caaacgcccg
     4621 gtgcgatcgc gctggcggaa gtgctggtcg atgaccgcct gatcgacgag gccaaaaccg
     4681 ccgaccatct gggccatgcg ctggcgcggg gtcggaccgc tcgcgccgaa ggcgaccatg
     4741 tgcgagctga ggatcgacgc gccggcgctg gttccagcca ccgtcgcgcc gccattccac
     4801 gctcgcttga tctcctgctc gacaagcgtg ccgccgagtc gggtcgagag cctgagctga
     4861 ttgccgccgg tgatgaagat acccgttgcg ttggcaaaga ccgtgttcca ttccggctcg
     4921 ttggcatggg cgcgatcgtg cacgaacgcg atgtgagccg agcgggcacc gaattcgaga
     4981 aaaatcgcct tgtaccgctc gccagccgtc tcgatcgacg aggcggtcgg gatgatcacg
     5041 atgcgggcgt cggccatgcc ggagagcgcc atgaaggtgg aaaggatcgt gcgctcgcgg
     5101 aacttgtcct ctgctccgcc aatagccatg atcgggccgg gatgtccatc ggctccgttg
     5161 taccgcacgc taccttcctg tcttcaatga tct
//
LOCUS       DATAOZ010000259         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2475383, whole genome shotgun sequence.
ACCESSION   DATAOZ010000259 DATAOZ010000000
VERSION     DATAOZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2475383"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..2030
                     /locus_tag="VHR64_06785"
     CDS             168..2030
                     /locus_tag="VHR64_06785"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX3302571.1"
                     /translation="MTHPLIDRLTSRTLLADGAMGTLIHEAGVPMGTCFDHLSLTNPD
                     LIGSIHRAYVSAGADIIETNTYGANQLKLAEFGLEHKVRDINFKAVKIAREAREVVGR
                     EVFVVGSVGPTRSAMDPLMPEEIKLLEPIYTEQIEALLEGGVDAICIETIADIREMAL
                     AVKAVRKASDLPIIASLAFGEDGTTPGGNAVNEVVATLLALDVDVIGANCSVGPNQML
                     PIIQAMTSLVSGTYDRQIPVSTMPNAGWPARVDGRYIFSSSPDYLGKFAAEAAVAGAK
                     LIGGCCGTTPDHTHAMRRTLDEQAGHSTLVIHEVRLPSTYEYEENGNDSPTELKRKLG
                     KDFVICVEIDPPKGMNPTKALDGARLLKDAGVTAINVADSPMARVRMSALMMSHLIQA
                     EVGVEAIVHFTTRDRSLMAIQSELLGAHAAGVRNILALTGDPPSLGDYPDSSAVYDID
                     SIGLIKVLSKMNEGVDWSGAPLGRNASFTIACAVDPTRNDLAREASRLHEKLDAGAHF
                     VMTQPIYDVEVWLRFLEHFGGKIPVPVMIGILPLQSARHAEFLHNEVPGITLTEQALE
                     RMRAAGNHGRQVGVEMARELLLDLKPHAQGVYLMPSFGRYEVAAEVLAGVVTPA"
BASE COUNT          386 a          666 c          665 g          377 t
ORIGIN      
        1 cactgatctc gacgtaacat tcattggtgg aatctaccgc gtggcgaaaa accggtcaaa
       61 ccgggtcagt tgccgcacgg tgtattcttt gcagaccatg tcggcgaatt cgccgcgttc
      121 actctggtac gagacgccag ccgggttttc gccaggagcc aactcccttg acacatcctt
      181 tgatcgaccg cctgacctcc cgcacgttgc tggcggatgg tgccatgggc acgctcatcc
      241 atgaggcggg cgtccctatg ggcacgtgct tcgatcacct gagcctgacc aatcccgacc
      301 tgattgggtc gattcaccgc gcctacgtct cggcgggcgc cgacatcatc gagaccaaca
      361 cctacggcgc gaaccagctc aaactggccg aatttggcct cgagcacaag gtccgggaca
      421 tcaacttcaa ggcggtcaag atcgcccggg aggctcgcga ggtcgtgggc cgtgaggtgt
      481 tcgtggtcgg ctcggtcggc ccgacccgct cggcgatgga cccgttgatg ccggaagaga
      541 tcaaactgct cgagccaatc tacacggagc agatcgaggc gctgcttgag ggtggcgtcg
      601 acgcgatctg cattgagaca atagccgaca tccgcgaaat ggcgctggcg gtcaaggctg
      661 tccgcaaggc ctcggacctg ccgatcatcg cctcgctcgc gtttggcgag gacggaacga
      721 caccaggcgg caacgcggtt aacgaagtcg tcgcgacgct gctcgcactc gacgtcgatg
      781 tgatcggcgc gaactgctcc gtcggtccga accagatgct gccgatcatc caggcgatga
      841 cgtcgctggt ctccggcacg tacgaccgcc agatccccgt ttcgacgatg ccaaacgcgg
      901 gttggccggc gcgggtcgat ggccggtaca tcttctcgag ctcaccggat tacctgggca
      961 agttcgcggc cgaggcagca gttgccggcg cgaaactgat cggcggctgc tgcgggacga
     1021 ctcccgatca cacccacgcg atgcgacgca ccctcgacga gcaggcgggg cactcgacgc
     1081 tcgtcatcca cgaggttcgc ctgccgtcga cttacgagta cgaggagaac ggcaacgatt
     1141 cgccgaccga gttgaagcgc aagctcggca aggacttcgt gatctgcgtc gagatcgacc
     1201 cgcccaaggg catgaacccg accaaggcgc tcgacggcgc gcggctcctc aaagacgccg
     1261 gagtgaccgc aatcaacgtc gccgactcgc cgatggcgcg agtccggatg agcgcgttga
     1321 tgatgtcgca cttgattcag gccgaagtgg gtgtcgaggc gatcgtccac ttcacgaccc
     1381 gggatcggag tctgatggct attcagagtg agctgctcgg cgcccatgcc gccggtgtcc
     1441 gcaacatcct ggccctgacc ggcgacccgc cgagcctggg cgattacccg gattcttccg
     1501 ctgtctacga catcgactcg atcggcctga tcaaggtcct gagcaagatg aacgagggcg
     1561 tggattggtc cggcgcgccg cttggccgca atgcgtcgtt cacgatcgcc tgcgccgtcg
     1621 atccgacacg caacgacctc gcgcgcgagg ccagccgact gcatgagaag ctggacgccg
     1681 gggctcattt cgtgatgacg cagccgatct acgacgtcga ggtctggctc cgcttcctcg
     1741 agcactttgg cggcaagatc ccggtaccgg tcatgatcgg catcctgccg ctgcaaagcg
     1801 cgcgccatgc cgagttcctg cacaacgagg tgccgggcat cacgctgacc gagcaggcgc
     1861 tggagcgaat gcgagctgcc gggaatcatg gccggcaggt cggcgtcgag atggcgcgcg
     1921 agttgctgct cgacctcaag ccgcatgcgc agggcgtcta cctcatgccg tccttcggac
     1981 ggtatgaggt cgctgcggag gtattggccg gggtggttac tcccgcctga tcataatgtg
     2041 gtagcattcg cagtcggctc gctcaactgc gggccgattc cgtgtatcta tcga
//
LOCUS       DATAOZ010000260        22192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9315589, whole genome shotgun sequence.
ACCESSION   DATAOZ010000260 DATAOZ010000000
VERSION     DATAOZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 22192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22192
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9315589"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VHR64_06790"
     CDS             complement(<1..1317)
                     /locus_tag="VHR64_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3302572.1"
                     /translation="MSLARANTSVFGYIGRRMALVNAAVVLLLIAVVGFGMVAVARYV
                     MIQQADQALKDQANVAALDWGPNLATGQPIPVADRPIDGDGDGHDGDDELEAEELLDS
                     GDLIAFGFDAQGTLVANPRGMRMTELPEQQAVAGALTGHDGYQTVSLHDEPDVRVYTM
                     PIEVDHQVVGAVQIVRNQKDLRSAIRAIELAALVAAAIGALGAVPTGLYLAKRAMRPI
                     DRAFRRQQAFIADASHELRTPLAVVRANTELVRRMPDATPQERDVELAAILEETDRMA
                     RLVDELLVLARSDAGRLELKSEVLDLSALVREAAEPMRALAAEAGQTLEIDAPDELLI
                     SGDEDRLAQILRILIGNAIAYTPAGGTITVKSSDVRHQTPVETAKKLTIHDSRLTTAT
                     LTVADTGIGMSAEERARVFDRFYRGEQSRSRGTGGVGLGLSIAQTIV"
     gene            complement(1304..2011)
                     /locus_tag="VHR64_06795"
     CDS             complement(1304..2011)
                     /locus_tag="VHR64_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3302573.1"
                     /translation="MLAHGSSIPETFYMRILIIEDEVRLAGIIARILTQERFTVDVIS
                     DGEEGLHAALTDAYDAIILDRMLPRLDGASIVRELRAEGNATPVLMLTALGDLPDRVE
                     GLNAGADDYLGKPFAFEELLARLRALVRRTDRPLAPDRVEVGPIAIDLRGRTVERNGK
                     PVDLSAREYALLELMARNRGQVLSKDTILERVWGIDAEPQGNVVELYVFYLRRKLGHT
                     AAHPVIRTVRGAGYVLG"
     gene            complement(2078..2470)
                     /locus_tag="VHR64_06800"
     CDS             complement(2078..2470)
                     /locus_tag="VHR64_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302574.1"
                     /translation="MNPTLKMAGMVSVALVGLLHLIEAPEYYGEVKYIGALFVLCAVG
                     AAVSVFGIWRDLRAGWLLGAVLAGSNFIAYLLSRTVGLPSFRENSWSQFAEPMGLLSL
                     AVEAVAFIICIRALRAAGAENNGRIALA"
     gene            complement(2735..3181)
                     /gene="tnpA"
                     /locus_tag="VHR64_06805"
     CDS             complement(2735..3181)
                     /gene="tnpA"
                     /locus_tag="VHR64_06805"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX3302575.1"
                     /translation="MSYWRLHYHLIWATKQRLPLIGSEEEAVIRESLEMTMARMKLIG
                     HAIGLVADHVHIALSIPPTVSVADAMGRLKGGSSHRVGQRLPKATGFAWQAEYGALSL
                     TERALPTVIEYVQNQKQHHAANTLIYGFERIESQAPRSRAIPQGAD"
     gene            complement(3358..4512)
                     /locus_tag="VHR64_06810"
     CDS             complement(3358..4512)
                     /locus_tag="VHR64_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302576.1"
                     /translation="MGITRRLFLGGGASLAAAMASGTRLKRRSARAQASAWEVVDLFA
                     LTEALSYRGNEYVGSFLDMNAAGEVGGSFVVSDVKISPAIWSPDGKATRLKSGKYGGE
                     VWALNNNGLAVGAGFLGVRGFESEESFYPGYLTPRAWKDGQPVELEVLGGDIKVSTGI
                     VQDLNDDGIMVGYLVDPALNVEVPVKWQDGAATVLAGGSPGGGFANQINSAGDIGGYA
                     RIDGTSTAVMWRGDKMDVLAPPDDPDLGENSVFLLGDLGDNGQILAVMNVPYEIDAST
                     IGYSNASYVLDKGKWTKLASPDETRQSVYGRSMNASGVVVGSINSTEEDVQALSAIWE
                     GGEGRILDEEVELPSGLKMAGVVKINDEGQILADARDADGTRHAVILRPA"
     gene            complement(4563..5678)
                     /locus_tag="VHR64_06815"
     CDS             complement(4563..5678)
                     /locus_tag="VHR64_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302577.1"
                     /translation="MRRLVLGILLVLPLLAGVLPASAQDARYEIKDLIKAHDIGNEAA
                     GLGAYDINDDGMIVGFTAQSATKSSPFYIVNGKTTRIKTGKFGATYGHVNADGVIVGR
                     DVTGRDENGGPIGLPAFWADGKTTRLELPLDPLGQTVVTGVAQEINDAGQIVGEVQYP
                     GDDFNQYAVLWQDGVPTLLPDAFGYPVCSAWGLTASGLVAGSCWEPNLGMFLPVLWQS
                     LVPISLDSQATANTDVTAATDTAAGGYAVVGYQYDDVDNPSYLEATVIIDGVIQSLPG
                     PAGYTACRAMTVSQTEAGLLVGGSCAPDVATGQKSWAGSVAVAWLDGELIELNDAIPQ
                     DQGWTLILIRGVNGKGQMVGLGERKGERRSFVLTPTE"
     gene            complement(5909..6853)
                     /locus_tag="VHR64_06820"
     CDS             complement(5909..6853)
                     /locus_tag="VHR64_06820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302578.1"
                     /translation="MTINGVSLVDFAAVLLATGLLRAVPLVVAGIGEAVAERAGLLNL
                     GIEGMMLSGCFFGFWAAYETDSLLIGMLAAVGSGLVLALLFGWLAITLKIDQILIGLA
                     ITIAGSGLTGYLFRDQFGGRNVAVDVDTSKIHVPWLKEIPVLGPALFEQQPLFYVSWA
                     LVPIVAWVLFRTRFGLQVRGAGETPVAVDAAGVSVERVRYVAIAIGGALAGFAGGFLC
                     VADVGIFTTNMTVGQGFIALALAMVGNWSPWRIAVGAIVFGVLRSMGDGLQILGVNTR
                     PEFLAMLPYLGVIVAMVLLAGRTRLPSALATPYRRGMR"
     gene            complement(6850..7890)
                     /locus_tag="VHR64_06825"
     CDS             complement(6850..7890)
                     /locus_tag="VHR64_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302579.1"
                     /translation="MISRITLQTQRSAATSSVLAVVGACLAGAILLLLTGHNPWTAYR
                     LIVERGLLDWDGLSESLKKMAPLLIITAGLLTCLRAGIWNIGIDGQVVIGAIACGVVA
                     GELVGDLPRALLLITGAVVGGLAGGLWALGPAVLTARFGLNEIITTIMMNYLAFNLVS
                     WLVKGPVKAPDIVTAQTKQIPLEDRLPDLPYTDIHIGLIVGVIAIVIVWLLFRETVPG
                     TMLDVLGRNRRAAKHAGLPVTRLIVGAFLVSGAAAGLAGAVDVLGVFGLFKATYQPGY
                     GFIAFALAYLARLRSIAVIPFAAFLSVLVIGGDAMSRRADVPSDFVPVLEGLMLLFFA
                     LAVWFEGRGSAA"
     gene            complement(7887..9419)
                     /locus_tag="VHR64_06830"
     CDS             complement(7887..9419)
                     /locus_tag="VHR64_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302580.1"
                     /translation="MTVVALSDIAKSFGEVQALHGVSLTLQRGEVHALLGENGAGKST
                     LLGILSGLVQPDAGTIALDSFPIRFETPADALTAGIGTVYQHFTLVPPMTVIENLRLG
                     LSRSGWMSSRELAKRLATIGIDLPLAARVEEIPVGQRQLVEIAKALLRRPRVLLLDEP
                     TSVLAGTEIAHLLELIRSIADHGTTVVLVTHKLDEALAIADRVTVLRRGRVSGEINLR
                     AATELRPSLTNRLIAMMFGGREPVAASVPHYAGPVDESRIVARLREVSARDNRGAPSL
                     RDFSLELCAGEVLGIAGVEGNGQQELAEILSGERHIDDGLLEINGRPMTNAGARALID
                     AGVGVTTDDRIAVGCVPGTSLAINLALKRIASPPFAEGVRLHKFEIRQFAQNLITRFD
                     IRPGNPDQPILELSGGNIQRALLARELAFRPDLLVCNQPTAGLDLLTAEQMLARIHKA
                     AGEGAAVLLISSDLDELLRHSDRIAVLYRGEIAGVVTRAEFDPDRIARLMVTGTEREP
                     AA"
     gene            9713..10390
                     /locus_tag="VHR64_06835"
     CDS             9713..10390
                     /locus_tag="VHR64_06835"
                     /inference="COORDINATES: protein motif:HMM:NF017530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HEX3302581.1"
                     /translation="MQTLAGDLLLLALDDDRGTVSWQRSTGLRYGLGGALLMDLAQQE
                     RIESLDDKIVLVDPSPTGDDVLDAALNAIATSNKPRDARYWVESLGGRSGLKEQLSRQ
                     LVARGILREQEREFLRVFHDHRFPTDDPGPESSLRVRLRDAALGATEPDTRTLLLLSL
                     VNACKLAGDLFSREERSQALSRIKELVEGEQFGKAVSKAIAAAAAATAAAASAAFTAT
                     VAPGASH"
     gene            complement(10456..10968)
                     /locus_tag="VHR64_06840"
     CDS             complement(10456..10968)
                     /locus_tag="VHR64_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX3302582.1"
                     /translation="MSIVPELMAANQRYAAQFANGDLPMPPRHKLVVLTCMDARIDPL
                     RALGLEEGDAHVLRNSGGRPTTDVIRSLAVSQEMLGTREVVVIHHTECGSIRITNDSI
                     RQRLTDSLGSDAARAAGEIDFLPFTDLEQSVRDDVATLQSSRLLRPETKISGFVFDVK
                     TGCLQQVDPT"
     gene            11225..11737
                     /locus_tag="VHR64_06845"
     CDS             11225..11737
                     /locus_tag="VHR64_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX3302583.1"
                     /translation="MSIVPELMAANQRYAAQFANGDLPMPPRHKLVVLTCMDARIDPL
                     RALGLEEGDAHVLRNSGGRPTTDVIRSLAVSQEMLGTREVVVIHHTECGSIRITNDSI
                     RQRLTDSLGSDAARAAGEIDFLPFTDLEQSVRDDVATLQSSRLLRPETKISGFVFDVK
                     TGCLQQVDPT"
     gene            complement(11803..12480)
                     /locus_tag="VHR64_06850"
     CDS             complement(11803..12480)
                     /locus_tag="VHR64_06850"
                     /inference="COORDINATES: protein motif:HMM:NF017530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HEX3302584.1"
                     /translation="MQTLAGDLLLLALDDDRGTVSWQRSTGLRYGLGGALLMDLAQQE
                     RIESLDDKIVLVDPSPTGDDVLDAALNAIATSNKPRDARYWVESLGGRSGLKEQLSRQ
                     LVARGILREQEREFLRVFHDHRFPTDDPGPESSLRVRLRDAALGATEPDTRTLLLLSL
                     VNACKLAGDLFSREERSQALSRIKELVEGEQFGKAVSKAIAAAAAATAAAASAAFTAT
                     VAPGASH"
     gene            12774..14306
                     /locus_tag="VHR64_06855"
     CDS             12774..14306
                     /locus_tag="VHR64_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302585.1"
                     /translation="MTVVALSDIAKSFGEVQALHGVSLTLQRGEVHALLGENGAGKST
                     LLGILSGLVQPDAGTIALDSFPIRFETPADALTAGIGTVYQHFTLVPPMTVIENLRLG
                     LSRSGWMSSRELAKRLATIGIDLPLAARVEEIPVGQRQLVEIAKALLRRPRVLLLDEP
                     TSVLAGTEIAHLLELIRSIADHGTTVVLVTHKLDEALAIADRVTVLRRGRVSGEINLR
                     AATELRPSLTNRLIAMMFGGREPVAASVPHYAGPVDESRIVARLREVSARDNRGAPSL
                     RDFSLELCAGEVLGIAGVEGNGQQELAEILSGERHIDDGLLEINGRPMTNAGARALID
                     AGVGVTTDDRIAVGCVPGTSLAINLALKRIASPPFAEGVRLHKFEIRQFAQNLITRFD
                     IRPGNPDQPILELSGGNIQRALLARELAFRPDLLVCNQPTAGLDLLTAEQMLARIHKA
                     AGEGAAVLLISSDLDELLRHSDRIAVLYRGEIAGVVTRAEFDPDRIARLMVTGTEREP
                     AA"
     gene            14303..15343
                     /locus_tag="VHR64_06860"
     CDS             14303..15343
                     /locus_tag="VHR64_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302586.1"
                     /translation="MISRITLQTQRSAATSSVLAVVGACLAGAILLLLTGHNPWTAYR
                     LIVERGLLDWDGLSESLKKMAPLLIITAGLLTCLRAGIWNIGIDGQVVIGAIACGVVA
                     GELVGDLPRALLLITGAVVGGLAGGLWALGPAVLTARFGLNEIITTIMMNYLAFNLVS
                     WLVKGPVKAPDIVTAQTKQIPLEDRLPDLPYTDIHIGLIVGVIAIVIVWLLFRETVPG
                     TMLDVLGRNRRAAKHAGLPVTRLIVGAFLVSGAAAGLAGAVDVLGVFGLFKATYQPGY
                     GFIAFALAYLARLRSIAVIPFAAFLSVLVIGGDAMSRRADVPSDFVPVLEGLMLLFFA
                     LAVWFEGRGSAA"
     gene            15340..16284
                     /locus_tag="VHR64_06865"
     CDS             15340..16284
                     /locus_tag="VHR64_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302587.1"
                     /translation="MTINGVSLVDFAAVLLATGLLRAVPLVVAGIGEAVAERAGLLNL
                     GIEGMMLSGCFFGFWAAYETDSLLIGMLAAVGSGLVLALLFGWLAITLKIDQILIGLA
                     ITIAGSGLTGYLFRDQFGGRNVAVDVDTSKIHVPWLKEIPVLGPALFEQQPLFYVSWA
                     LVPIVAWVLFRTRFGLQVRGAGETPVAVDAAGVSVERVRYVAIAIGGALAGFAGGFLC
                     VADVGIFTTNMTVGQGFIALALAMVGNWSPWRIAVGAIVFGVLRSMGDGLQILGVNTR
                     PEFLAMLPYLGVIVAMVLLAGRTRLPSALATPYRRGMR"
     gene            16515..17630
                     /locus_tag="VHR64_06870"
     CDS             16515..17630
                     /locus_tag="VHR64_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302588.1"
                     /translation="MRRLVLGILLVLPLLAGVLPASAQDARYEIKDLIKAHDIGNEAA
                     GLGAYDINDDGMIVGFTAQSATKSSPFYIVNGKTTRIKTGKFGATYGHVNADGVIVGR
                     DVTGRDENGGPIGLPAFWADGKTTRLELPLDPLGQTVVTGVAQEINDAGQIVGEVQYP
                     GDDFNQYAVLWQDGVPTLLPDAFGYPVCSAWGLTASGLVAGSCWEPNLGMFLPVLWQS
                     LVPISLDSQATANTDVTAATDTAAGGYAVVGYQYDDVDNPSYLEATVIIDGVIQSLPG
                     PAGYTACRAMTVSQTEAGLLVGGSCAPDVATGQKSWAGSVAVAWLDGELIELNDAIPQ
                     DQGWTLILIRGVNGKGQMVGLGERKGERRSFVLTPTE"
     gene            17681..18835
                     /locus_tag="VHR64_06875"
     CDS             17681..18835
                     /locus_tag="VHR64_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302589.1"
                     /translation="MGITRRLFLGGGASLAAAMASGTRLKRRSARAQASAWEVVDLFA
                     LTEALSYRGNEYVGSFLDMNAAGEVGGSFVVSDVKISPAIWSPDGKATRLKSGKYGGE
                     VWALNNNGLAVGAGFLGVRGFESEESFYPGYLTPRAWKDGQPVELEVLGGDIKVSTGI
                     VQDLNDDGIMVGYLVDPALNVEVPVKWQDGAATVLAGGSPGGGFANQINSAGDIGGYA
                     RIDGTSTAVMWRGDKMDVLAPPDDPDLGENSVFLLGDLGDNGQILAVMNVPYEIDAST
                     IGYSNASYVLDKGKWTKLASPDETRQSVYGRSMNASGVVVGSINSTEEDVQALSAIWE
                     GGEGRILDEEVELPSGLKMAGVVKINDEGQILADARDADGTRHAVILRPA"
     gene            19012..19458
                     /gene="tnpA"
                     /locus_tag="VHR64_06880"
     CDS             19012..19458
                     /gene="tnpA"
                     /locus_tag="VHR64_06880"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEX3302590.1"
                     /translation="MSYWRLHYHLIWATKQRLPLIGSEEEAVIRESLEMTMARMKLIG
                     HAIGLVADHVHIALSIPPTVSVADAMGRLKGGSSHRVGQRLPKATGFAWQAEYGALSL
                     TERALPTVIEYVQNQKQHHAANTLIYGFERIESQAPRSRAIPQGAD"
     gene            19723..20115
                     /locus_tag="VHR64_06885"
     CDS             19723..20115
                     /locus_tag="VHR64_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302591.1"
                     /translation="MNPTLKMAGMVSVALVGLLHLIEAPEYYGEVKYIGALFVLCAVG
                     AAVSVFGIWRDLRAGWLLGAVLAGSNFIAYLLSRTVGLPSFRENSWSQFAEPMGLLSL
                     AVEAVAFIICIRALRAAGAENNGRIALA"
     gene            20182..20889
                     /locus_tag="VHR64_06890"
     CDS             20182..20889
                     /locus_tag="VHR64_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3302592.1"
                     /translation="MLAHGSSIPETFYMRILIIEDEVRLAGIIARILTQERFTVDVIS
                     DGEEGLHAALTDAYDAIILDRMLPRLDGASIVRELRAEGNATPVLMLTALGDLPDRVE
                     GLNAGADDYLGKPFAFEELLARLRALVRRTDRPLAPDRVEVGPIAIDLRGRTVERNGK
                     PVDLSAREYALLELMARNRGQVLSKDTILERVWGIDAEPQGNVVELYVFYLRRKLGHT
                     AAHPVIRTVRGAGYVLG"
     gene            20876..>22192
                     /locus_tag="VHR64_06895"
     CDS             20876..>22192
                     /locus_tag="VHR64_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3302593.1"
                     /translation="MSLARANTSVFGYIGRRMALVNAAVVLLLIAVVGFGMVAVARYV
                     MIQQADQALKDQANVAALDWGPNLATGQPIPVADRPIDGDGDGHDGDDELEAEELLDS
                     GDLIAFGFDAQGTLVANPRGMRMTELPEQQAVAGALTGHDGYQTVSLHDEPDVRVYTM
                     PIEVDHQVVGAVQIVRNQKDLRSAIRAIELAALVAAAIGALGAVPTGLYLAKRAMRPI
                     DRAFRRQQAFIADASHELRTPLAVVRANTELVRRMPDATPQERDVELAAILEETDRMA
                     RLVDELLVLARSDAGRLELKSEVLDLSALVREAAEPMRALAAEAGQTLEIDAPDELLI
                     SGDEDRLAQILRILIGNAIAYTPAGGTITVKSSDVRHQTPVETAKKLTIHDSRLTTAT
                     LTVADTGIGMSAEERARVFDRFYRGEQSRSRGTGGVGLGLSIAQTIV"
BASE COUNT         3831 a         7265 c         7265 g         3831 t
ORIGIN      
        1 gacgatggtc tgcgcgatcg agaggccgag cccgacgccg cccgtgcccc ggctgcgcga
       61 ttgctcgcca cgatagaacc ggtcgaagac ccgcgcccgc tcctccgcgc tcattccgat
      121 gccggtatcg gcgacggtga gggtcgcagt cgtcagtcgt gagtcgtgaa tcgtgagttt
      181 ctttgccgtc tcaactggcg tctgatgtct gacgtctgac gacttcacag tgatcgttcc
      241 gcccgccggc gtgtaggcga tcgcgttgcc gatgaggatg cgcaggatct gggccaggcg
      301 gtcttcgtcg ccgctgatca ggagctcgtc cggcgcgtcg atctcgagcg tttggccggc
      361 ctcggccgcc agcgcgcgca tcggctctgc cgcctcccgc accagcgccg agagatcgag
      421 cacctccgac ttcagctcca gccggccggc atcggaccgc gccagcacca gcagctcatc
      481 gaccagccgg gccatccggt ccgtctcctc gaggatcgcg gccaactcga cgtcccgctc
      541 ctgcggcgtc gcgtcgggca tccgccggac cagctcggtg ttggcccgga cgaccgccag
      601 cggcgtgcgc aactcgtgcg aagcgtcggc gatgaaggcc tgctggcgac ggaaggcccg
      661 gtcgatcggt cgcatcgccc gcttcgccag gtacagcccg gtcggaaccg ccccgagtgc
      721 gccgatcgcc gccgcgacca gtgccgccaa ctcgatcgcc cggatcgctg agcgcagatc
      781 cttctggttg cgcacgatct ggaccgcgcc gaccacctgg tgatcgacct cgatcggcat
      841 cgtgtagacg cggacatccg gctcatcgtg caggctgacg gtctggtacc cgtcgtggcc
      901 ggtcagggct cccgcaactg cctgctgctc gggcaactcg gtcatccgca tcccgcgcgg
      961 gttggcgacc agcgtgccct gggcgtcgaa gccgaacgcg atcagatcgc cgctatcgag
     1021 cagctcctcg gcctccagct catcgtcgcc atcgtgccca tccccgtcac cgtcgatcgg
     1081 ccggtccgca accggaatcg gctggccggt ggcgagattc gggccccaat cgagggcggc
     1141 gacattggcc tgatccttga gtgcctgatc cgcctgctga atcatcacgt agcgtgccac
     1201 cgccaccatc ccgaagccga cgaccgcgat caggagcagg acgacggccg cgttgaccag
     1261 tgccatccgc cggccgatgt agccgaagac gctcgtattg gccctagcca aggacatagc
     1321 cggctcccct gaccgtgcgg atgacaggat gcgcggcggt gtggcccagc ttgcggcgca
     1381 ggtagaagac gtagagctcg accacgttgc cctgcggctc ggcatcgatt ccccagacgc
     1441 gctcgaggat cgtgtccttg ctcaggactt ggcccctgtt acgggccatc agctcgagca
     1501 gggcgtactc ccgcgccgag agatcgaccg gcttcccgtt ccgctccacg gtccggccgc
     1561 gcaggtcgat cgcgatcggg ccgacctcga cccggtcggg cgcgagcggg cgatcggtcc
     1621 ggcggaccag agcgcgcagg cgggcgagca gctcctcaaa ggcgaacggc ttgccgaggt
     1681 aatcgtcggc gccggcgttc agcccttcga cccggtccgg caggtcgccg agcgcagtca
     1741 gcatcaggac gggcgtggcg ttgccctcgg cgcggagctc tcggacgatg ctggcaccgt
     1801 cgagccgggg cagcatgcgg tcgaggatga tcgcgtcgta ggcgtcggtc agggcggcgt
     1861 gcaggccctc ttcgccatcg gagatgacat cgaccgtgaa tcgctcctgg gtcaggatcc
     1921 gggcgatgat cccggccagc cgcacttcgt cttcgatgat caggatccgc atgtagaacg
     1981 tctcagggat agagctgcca tgggcaagca tacgccgatg ccgcgcggat cgggcgcggc
     2041 atcggtgtgc gttgggaagg ggaaggaagg gcttggctca ggcaagggcg atccggccgt
     2101 tgttctccgc gccggcggca cggagggcac ggatgcagat gatgaaggcg acagcctcga
     2161 cggcgaggga gagcagcccc atcggctcgg cgaactgaga ccacgagttc tcgcggaagc
     2221 tcggcaggcc gacggtgcgg ctgaggagat aagcgatgaa gttgctgccg gcgaggaccg
     2281 cgccgagcag ccagcccgcg cgcaggtccc tccagatgcc gaacacggag accgccgcgc
     2341 caaccgcgca gagcacgaac agcgcgccga tgtacttgac ctcgccgtaa tactccggcg
     2401 cctcaatcag gtgcagcagc ccgaccaggg ccaccgacac catgccagcc atcttgaggg
     2461 tcggattcac gcctctcatt tccatacgac aaccactcca aacacgcgga acgggtcgcg
     2521 ccagccaggc ggaggacccg ttcgaaaccg ggcgtccggc tactcatttg ccggactgaa
     2581 agtcactctg gcagccccgg attagagcga gatttgagcg agtcatggga cgtgagtcgt
     2641 gagcagggaa tatgcgtgag tcgagtggag attggcggga ttgcccgggc ctgaagaccc
     2701 gggctcaaac ctgaacccgg ctaaagccgg ctcctcagtc ggcgccttgc ggtatggctc
     2761 tagatcttgg agcttggctt tcgatgcgct cgaaaccata gatcaatgtg ttcgcggcgt
     2821 ggtgctgttt ctggttctgc acgtactcga tcacggtcgg caaggcacgt tcggtgaggc
     2881 tgagggcgcc gtattccgct tgccaggcaa atccagttgc tttaggcagg cgctggccaa
     2941 cacgatgtga tgatccgcct tttagccgcc ccatggcgtc ggcgacggaa acagtgggtg
     3001 gtatggaaag cgcgatatgc acgtggtctg ccacgagtcc tatcgcatga ccgatcaact
     3061 tcatgcgtgc catggtcatt tccagggact cgcggatgac cgcctcttcc tccgaaccaa
     3121 tgaggggaag gcgctgtttg gtcgcccaaa tgaggtgata gtgcaagcgc caatacgaca
     3181 taccggcatt atctgaaatc tcattgatta ttgcccgccg tcccagccgg cttcagccgg
     3241 gttcatggtt cagcccgggc ctgaagaccc gggctgcgtt actgtctgtc ccgttccgtc
     3301 gactctccac ctgcttccct tgcaatgcag agtggcgcgg tccgaacaat gcttggtcta
     3361 cgctggccgc aagatgacgg cgtggcgtgt cccatcggcg tccctggcgt ccgcaagaat
     3421 ctggccctcg tcgttgatct tgacgacgcc cgccatcttc agcccgctcg gcagttccac
     3481 ttcttcgtcg agaatgcgcc cctcgccgcc ctcccagatc gcgctgaggg cctggacgtc
     3541 ctcctcggtc gagttgatcg accccacaac gacgccactc gcattcatcg agcggccgta
     3601 cacgctctgg cgtgtctcgt ccgggctggc cagcttggtc cacttaccct tgtcgagcac
     3661 gtaggacgca ttggagtacc cgatggtgga cgcatcgatc tcatacggga cgttcataac
     3721 cgcaaggatc tggccattgt cgccgaggtc gcccaggagg aagacggagt tctcccccag
     3781 gtcggggtcg tccgggggcg ccagcacgtc catcttgtcg ccccgccaca tgacggccgt
     3841 gctggtgccg tcgattctgg cgtacccgcc gatgtcaccg gcgctgttga tctggttggc
     3901 aaagccaccg cccggactcc caccggccag caccgtcgcc gcgccatcct gccatttaac
     3961 cggcacttcc acgttaagcg caggatcgac gagatacccg accatgatcc cgtcgtcgtt
     4021 gaggtcctga acgatgccgg tcgatacctt tatgtcacct cccaggacct ccagctcgac
     4081 cggttgacca tctttccagg cccgtggggt caagtaacca gggtaaaacg attcctctga
     4141 ctcgaagccg cgtacgccaa gaaagccggc gccgactgcg agtccattgt tgttaagcgc
     4201 ccacacttcg ccgccgtact taccgctctt gaggcgcgtc gccttgccat caggagacca
     4261 gattgccggc gaaatcttga cgtcggagac gacgaaggag ccaccgacct cccccgcggc
     4321 gttcatatcc aggaaggagc ccacatactc gttaccgcga taagaaagcg cctcggtcaa
     4381 cgcaaaaaga tcgacaacct cccaggcact cgcctgtgcc cttgctgagc gccgtttcaa
     4441 tctcgtgccc gaagccatcg ccgccgcaag cgacgcacca ccaccgagga agagtcgccg
     4501 tgtaattccc atctgaatcg tttctccaag tccaattgat catttcaaga acgacggtgc
     4561 tgctactcag ttggtgtcag cacgaatgac ctccgctctc ccttccgctc acccagtcca
     4621 accatttgac ccttgccgtt gacaccgcgg atcaggatca gggtccagcc ctgatcctgc
     4681 ggaatggcgt cgttaagctc gatcagttca ccgtccagcc acgccaccgc cacactgccg
     4741 gcccaactct tctggccagt ggcgacgtcc ggcgcgcagg atccgcccac cagcaatccg
     4801 gcctctgtct ggctgaccgt catcgcgcgg caggcggtat atccggccgg gccgggcagg
     4861 gactggataa cgccatcgat gatgacggtg gcctcgaggt aggaggggtt atccacgtca
     4921 tcgtattggt acccgacgac ggcataaccc cccgccgcgg tgtccgtggc ggccgtcaca
     4981 tccgtgttgg cggtcgcctg ggaatcgagc gagattggca ccagtgattg ccagaggacc
     5041 ggcagaaaca tgcccaggtt cggctcccag cacgacccgg cgaccagccc ggatgcggtc
     5101 aggccccagg cggagcagac cgggtaaccg aaggcatcgg gcagcagggt cggcacaccg
     5161 tcctgccaga gcaccgcgta ctggttgaag tcgtcgcccg gatactggac ttcgcccacg
     5221 atttgcccgg cgtcgttgat ctcctgggcg acaccggtga cgactgtttg cccgagcgga
     5281 tcgagcggca gctctagccg ggtcgttttg ccatcggccc agaatgccgg taagccgatc
     5341 ggtccgccgt tctcatcgcg accggtgacg tcgcgaccga cgatcacccc gtcggcgttg
     5401 acatgtccat aggtggctcc gaacttaccg gtcttgatcc gggttgtctt gccgttgacg
     5461 atgtagaagg gactggattt ggtggcggat tgagcagtga aaccgacgat cataccgtcg
     5521 tcgttgatgt cgtacgcgcc gaggccggct gcctcgttcc cgatgtcgtg ggccttgatc
     5581 agatccttga tctcgtagcg cgcatcctgg gccgatgcgg gcagaacacc ggcaagcaga
     5641 ggcaaaacaa gcaggatccc aagaacgagt ctccgcacga tcatctccct caagtcgaca
     5701 cccggcgtcg gcgatacacc agatacgcag ataccgaggc gactttcgcc ccggtatgtc
     5761 cgaccgctcc gcgagccccg tcgttttgca gggccgaatt tcacttggac ggtacttttg
     5821 gcgatcctat catgcggaat acaaagcgac catatagcag tttggtccta ggagctgagc
     5881 cgttagcgga cgctacattt cctcgccatc accgcatgcc tctccggtac ggcgtggcta
     5941 acgcggaggg gagtcttgtg cggccggcga ggaggaccat ggcgacgatc acgccgaggt
     6001 aaggcagcat cgccaggaac tcgggtcgcg tgttcacccc caggatctgc aacccgtcgc
     6061 ccatcgagcg cagcacgccg aagacgatcg ccccaaccgc gatccgccac gggctccagt
     6121 tcccgaccat cgccagggcc agcgcgatga agccctgccc gacggtcatg ttggtggtga
     6181 agattccgac atcggccacg cacaggaagc cacccgcgaa tccggctagc gccccgccga
     6241 tcgcgatcgc cacgtaccgc acccgctcga ccgagacccc ggccgcatcg accgccaccg
     6301 gcgtctcgcc tgcgccgcgc acctgcagac cgaagcgagt ccggaacagc acccaggcca
     6361 cgatcgggac cagcgcccag ctcacgtaga agagcggctg ctgctcgaac agggccgggc
     6421 cgagcaccgg gatctccttc agccagggca cgtggatctt gctggtgtcg acatcgaccg
     6481 ccacatttcg accgccgaac tggtcccgga agagatagcc ggtcagccca ctcccggcga
     6541 tcgtgatggc gagaccgatc aggatttggt cgatcttgag cgtgatcgcc agccagccga
     6601 agagcagcgc cagcacgagc cctgacccga ccgccgccag catcccgatc agcaggctgt
     6661 cggtctcgta cgccgcccag aagccgaaga agcagccgct cagcatcatc ccctcgatgc
     6721 cgaggttgag cagtccggcc cgctcggcga ccgcttcgcc gatcccggcc acgaccagcg
     6781 gcaccgcccg gagcaggcca gtcgcgagca ggaccgccgc gaaatcgacg agagagacgc
     6841 cgttgatggt catgccgcgc tccctcgtcc ctcgaaccag accgccagcg cgaagaagag
     6901 cagcatcagt ccctcgagca cggggacaaa gtcggacggc acatcggccc ggcggctcat
     6961 cgcatcgccg ccgatcacaa gcaccgacag gaaggcggcg aaggggatca ccgcgatcga
     7021 tcgcagccgg gcaaggtagg ccagcgcgaa ggcgatgaag ccgtagcccg gctggtaggt
     7081 cgccttgaag agaccgaaaa ccccgagcac gtcgactgcc ccggccaggc ccgccgccgc
     7141 cccggagacg aggaacgcgc cgacaatcag ccgggttacg ggcaaaccgg catgcttcgc
     7201 ggcccgccgg ttccggccga ggacatccag catcgtcccc ggcacggtct cacggaagag
     7261 cagccagacg atcacgatcg cgatcacccc gacgatcagg ccgatgtgga tgtcggtgta
     7321 gggcaaatca ggcagccggt cctcgagcgg gatctgcttc gtctgcgcgg tcacgatatc
     7381 cggcgccttg accggcccct tgaccagcca gctgacgagg ttgaaggcga ggtagttcat
     7441 catgatcgtg gtgatgatct cgttcaggcc gaagcgcgcg gtcagcaccg ccggcccgag
     7501 cgcccagagc ccaccggcca gaccgccgac caccgcaccg gtgatcagca ggagcgcccg
     7561 cggcaaatcc ccgaccaact cccccgcgac gaccccgcag gcgatcgccc cgatcacgac
     7621 ctggccgtcg atgccgatgt tccagatccc ggcgcgcagg caggtcagca ggccggcggt
     7681 tatgatcagg aggggtgcca tcttcttgag cgactcgctc agcccgtccc agtcgagcag
     7741 gcctcgctcg acgatcagcc gataggccgt ccatgggttg tggccggtca ggagcaggag
     7801 gatcgccccc gccaggcagg cgccgacgac ggccaggacc gagctggtcg cggccgagcg
     7861 ctgggtttgc agcgtgatcc ggctgatcat gcggccggct cccgctcggt cccggtcacc
     7921 atcagtcgcg cgatccggtc cggatcgaac tcagcgcgag tcacgacgcc ggcgatctcg
     7981 ccccggtaga gcaccgcgat ccggtcggag tgacggagca gctcatcgag gtcggaggag
     8041 atcagcagca ccgccgcgcc ctctcccgcc gccttatgga tccgggccag catctgctcg
     8101 gcggtcagca aatccagccc ggcggtcggc tggttgcaga cgagcagatc gggccggaag
     8161 gcgagctccc gggccagcag cgcgcgctgg atgttgccac cggagagctc gaggatcggc
     8221 tgatccgggt tgcccggccg gatatcgaaa cgggtgatca ggttctgggc aaactggcgg
     8281 atctcgaact tgtgcagccg cactccctcg gcgaatggcg gcgaggcgat ccgcttcagc
     8341 gcgaggttga tcgccaggct cgtccccggc acgcagccga ccgcgatccg gtcgtcggtc
     8401 gtcacgccga cgccggcgtc gatcagggcg cgggctccgg cgttcgtcat cggccggccg
     8461 ttgatctcga gcaggccgtc atcgatgtgg cgctcgccgg agaggatttc ggccagctcc
     8521 tgctgcccgt tgccctcgac tccggcgata ccgagcacct cgccggcgca gagctccagc
     8581 gagaagtcgc gcaaggacgg cgcgccgcga ttgtcccgcg ccgagacctc ccgcagacgc
     8641 gccacgatgc gggactcgtc aaccgggccg gcataatgcg gaaccgatgc cgcgaccggc
     8701 tcccgtccgc cgaacatcat cgcgatcagc cgattggtca ggctcggccg gagctcggtc
     8761 gcggcccgca aattgatctc gccgctgacc cgtccccgcc gcaggaccgt cactcggtcg
     8821 gcaatggcga gggcctcgtc gagcttgtgc gtgaccagga cgacggtggt gccgtggtcg
     8881 gcgatcgagc ggattagctc gagcaggtgc gcaatctccg tcccggccag gaccgaggtc
     8941 ggctcgtcga ggaggaggac gcgcggccgc cgcagcagcg ccttagcgat ctcgaccagc
     9001 tgccgttgcc cgaccgggat ctcctcgacc cgcgcggcca gtggcaggtc gatcccgatc
     9061 gttgcgagcc gcttcgccag ctcgcgcgag ctcatccagc cggaccggga gaggccgagg
     9121 cgcagattct cgatgaccgt catcggcggg acgagggtga agtgctggta gaccgtgccg
     9181 atcccagccg tgagggcatc ggccggcgtc tcgaaccgga tcgggaagct gtcgagggcg
     9241 atcgtgccgg catcgggttg gacgaggccg gagaggatac cgagcagggt cgatttgccg
     9301 gctccgttct cgccgagcag agcatggacc tcaccgcgct ggagggtgag cgagacccca
     9361 tgcagcgcct gcacctcgcc gaatgatttc gcgatgtcgg agagcgcaac gacggtcatg
     9421 cggggatgat aaggcgggtt gagggtggag agtggagggt tgaggaaaaa ggacgacggg
     9481 tagcgtcgca tagcaacgcg actcgggttt ctccacggcc agttcggtgc tcacgccacc
     9541 ggactgaggc gcatttgcca cgtccatcga ggtaatgagg tcgcgcctgg gatccgttcc
     9601 tggatagcgt gcgatcgacg gatcgcggac caccgtgccc gcccagctcc tgaaaattgc
     9661 gcgccgtcag ccggtttgga gccgatggag tcacaagcct tggaggctta ccatgcaaac
     9721 gttagcagga gatttgttgc tactcgcact cgacgatgac agggggacgg tctcctggca
     9781 gcgctcgact ggcctccgat acgggcttgg cggggcgctc ctgatggatc tggcgcagca
     9841 ggagcgcatc gaaagtctcg acgacaagat cgtgctcgtc gatccgtctc cgacgggaga
     9901 cgacgtactg gatgccgccc tgaatgccat cgccacctca aacaaacccc gtgatgccag
     9961 gtattgggtg gaatcgctcg gcggccggtc gggactgaag gaacaacttt cccgtcagtt
    10021 ggtggcacgc ggcatcttgc gcgagcagga acgtgaattc cttagggtct ttcacgatca
    10081 tcgcttcccc actgatgatc ctggcccgga atcatcactc cgggtccggc tccgcgatgc
    10141 ggctcttggt gccaccgagc cggatacacg caccctgctg ctgttgagcc tggtcaatgc
    10201 ctgtaagctc gcgggcgacc tgttctcacg ggaagagcgc tcgcaggcct tgtcacggat
    10261 caaggagctg gtcgagggcg agcagttcgg caaggccgtc agtaaagcaa tcgccgcagc
    10321 cgcagcagca acggcggccg cagcgtctgc tgcgttcacg gcgaccgtgg cccctggtgc
    10381 atcgcactga cggctgaagc cggaatacgt cacggttgag agaacggaac cgacgtcggg
    10441 atccgccgca tgctcctacg taggatcaac ctgctgcagg cagcctgtct tgacgtcaaa
    10501 gacgaagccg ctgattttgg tttcggggcg gaggagacga gaggattgca gggtggccac
    10561 gtcgtcgcgg acgctttgct cgagatcggt gaagggcagg aagtcgatct cgccggccgc
    10621 gcgagcagcg tccgagccga gactgtcggt caggcgctgg cggatcgagt cgttggtgat
    10681 ccgaatcgag ccgcactcgg tgtgatgaat gaccacgacc tcgcgcgtgc ccagcatctc
    10741 ctgcgagacc gcgagcgagc gaatcacgtc ggtcgtcggg cggccaccgg agttgcgcag
    10801 gacgtgcgcg tcgccctcct ccaggccgag tgcccggagc ggatcgattc gcgcgtccat
    10861 gcaggtcagc accacgagct tgtggcgcgg cggcatcggc aagtcaccat tggcaaactg
    10921 cgcggcatac cgctggttgg cggccatcaa ctcgggcacg atcgacatgg gcggttttct
    10981 ccggcaagtc aggcgcaggc cgggaccacc cccgaccgcg cctgagtgta ccgatccgaa
    11041 gccttatcgc gcgtttcccg gcgcaccaaa gccgcaggcc atgcagggtc gcatggccat
    11101 gcgaccctgc atggcctgcg gctttggtgc gccgggaaac gcgcgataag gcttcggatc
    11161 ggtacactca ggcgcggtcg ggggtggtcc cggcctgcgc ctgacttgcc ggagaaaacc
    11221 gcccatgtcg atcgtgcccg agttgatggc cgccaaccag cggtatgccg cgcagtttgc
    11281 caatggtgac ttgccgatgc cgccgcgcca caagctcgtg gtgctgacct gcatggacgc
    11341 gcgaatcgat ccgctccggg cactcggcct ggaggagggc gacgcgcacg tcctgcgcaa
    11401 ctccggtggc cgcccgacga ccgacgtgat tcgctcgctc gcggtctcgc aggagatgct
    11461 gggcacgcgc gaggtcgtgg tcattcatca caccgagtgc ggctcgattc ggatcaccaa
    11521 cgactcgatc cgccagcgcc tgaccgacag tctcggctcg gacgctgctc gcgcggccgg
    11581 cgagatcgac ttcctgccct tcaccgatct cgagcaaagc gtccgcgacg acgtggccac
    11641 cctgcaatcc tctcgtctcc tccgccccga aaccaaaatc agcggcttcg tctttgacgt
    11701 caagacaggc tgcctgcagc aggttgatcc tacgtaggag catgcggcgg atcccgacgt
    11761 cggttccgtt ctctcaaccg tgacgtattc cggcttcagc cgtcagtgcg atgcaccagg
    11821 ggccacggtc gccgtgaacg cagcagacgc tgcggccgcc gttgctgctg cggctgcggc
    11881 gattgcttta ctgacggcct tgccgaactg ctcgccctcg accagctcct tgatccgtga
    11941 caaggcctgc gagcgctctt cccgtgagaa caggtcgccc gcgagcttac aggcattgac
    12001 caggctcaac agcagcaggg tgcgtgtatc cggctcggtg gcaccaagag ccgcatcgcg
    12061 gagccggacc cggagtgatg attccgggcc aggatcatca gtggggaagc gatgatcgtg
    12121 aaagacccta aggaattcac gttcctgctc gcgcaagatg ccgcgtgcca ccaactgacg
    12181 ggaaagttgt tccttcagtc ccgaccggcc gccgagcgat tccacccaat acctggcatc
    12241 acggggtttg tttgaggtgg cgatggcatt cagggcggca tccagtacgt cgtctcccgt
    12301 cggagacgga tcgacgagca cgatcttgtc gtcgagactt tcgatgcgct cctgctgcgc
    12361 cagatccatc aggagcgccc cgccaagccc gtatcggagg ccagtcgagc gctgccagga
    12421 gaccgtcccc ctgtcatcgt cgagtgcgag tagcaacaaa tctcctgcta acgtttgcat
    12481 ggtaagcctc caaggcttgt gactccatcg gctccaaacc ggctgacggc gcgcaatttt
    12541 caggagctgg gcgggcacgg tggtccgcga tccgtcgatc gcacgctatc caggaacgga
    12601 tcccaggcgc gacctcatta cctcgatgga cgtggcaaat gcgcctcagt ccggtggcgt
    12661 gagcaccgaa ctggccgtgg agaaacccga gtcgcgttgc tatgcgacgc tacccgtcgt
    12721 cctttttcct caaccctcca ctctccaccc tcaacccgcc ttatcatccc cgcatgaccg
    12781 tcgttgcgct ctccgacatc gcgaaatcat tcggcgaggt gcaggcgctg catggggtct
    12841 cgctcaccct ccagcgcggt gaggtccatg ctctgctcgg cgagaacgga gccggcaaat
    12901 cgaccctgct cggtatcctc tccggcctcg tccaacccga tgccggcacg atcgccctcg
    12961 acagcttccc gatccggttc gagacgccgg ccgatgccct cacggctggg atcggcacgg
    13021 tctaccagca cttcaccctc gtcccgccga tgacggtcat cgagaatctg cgcctcggcc
    13081 tctcccggtc cggctggatg agctcgcgcg agctggcgaa gcggctcgca acgatcggga
    13141 tcgacctgcc actggccgcg cgggtcgagg agatcccggt cgggcaacgg cagctggtcg
    13201 agatcgctaa ggcgctgctg cggcggccgc gcgtcctcct cctcgacgag ccgacctcgg
    13261 tcctggccgg gacggagatt gcgcacctgc tcgagctaat ccgctcgatc gccgaccacg
    13321 gcaccaccgt cgtcctggtc acgcacaagc tcgacgaggc cctcgccatt gccgaccgag
    13381 tgacggtcct gcggcgggga cgggtcagcg gcgagatcaa tttgcgggcc gcgaccgagc
    13441 tccggccgag cctgaccaat cggctgatcg cgatgatgtt cggcggacgg gagccggtcg
    13501 cggcatcggt tccgcattat gccggcccgg ttgacgagtc ccgcatcgtg gcgcgtctgc
    13561 gggaggtctc ggcgcgggac aatcgcggcg cgccgtcctt gcgcgacttc tcgctggagc
    13621 tctgcgccgg cgaggtgctc ggtatcgccg gagtcgaggg caacgggcag caggagctgg
    13681 ccgaaatcct ctccggcgag cgccacatcg atgacggcct gctcgagatc aacggccggc
    13741 cgatgacgaa cgccggagcc cgcgccctga tcgacgccgg cgtcggcgtg acgaccgacg
    13801 accggatcgc ggtcggctgc gtgccgggga cgagcctggc gatcaacctc gcgctgaagc
    13861 ggatcgcctc gccgccattc gccgagggag tgcggctgca caagttcgag atccgccagt
    13921 ttgcccagaa cctgatcacc cgtttcgata tccggccggg caacccggat cagccgatcc
    13981 tcgagctctc cggtggcaac atccagcgcg cgctgctggc ccgggagctc gccttccggc
    14041 ccgatctgct cgtctgcaac cagccgaccg ccgggctgga tttgctgacc gccgagcaga
    14101 tgctggcccg gatccataag gcggcgggag agggcgcggc ggtgctgctg atctcctccg
    14161 acctcgatga gctgctccgt cactccgacc ggatcgcggt gctctaccgg ggcgagatcg
    14221 ccggcgtcgt gactcgcgct gagttcgatc cggaccggat cgcgcgactg atggtgaccg
    14281 ggaccgagcg ggagccggcc gcatgatcag ccggatcacg ctgcaaaccc agcgctcggc
    14341 cgcgaccagc tcggtcctgg ccgtcgtcgg cgcctgcctg gcgggggcga tcctcctgct
    14401 cctgaccggc cacaacccat ggacggccta tcggctgatc gtcgagcgag gcctgctcga
    14461 ctgggacggg ctgagcgagt cgctcaagaa gatggcaccc ctcctgatca taaccgccgg
    14521 cctgctgacc tgcctgcgcg ccgggatctg gaacatcggc atcgacggcc aggtcgtgat
    14581 cggggcgatc gcctgcgggg tcgtcgcggg ggagttggtc ggggatttgc cgcgggcgct
    14641 cctgctgatc accggtgcgg tggtcggcgg tctggccggt gggctctggg cgctcgggcc
    14701 ggcggtgctg accgcgcgct tcggcctgaa cgagatcatc accacgatca tgatgaacta
    14761 cctcgccttc aacctcgtca gctggctggt caaggggccg gtcaaggcgc cggatatcgt
    14821 gaccgcgcag acgaagcaga tcccgctcga ggaccggctg cctgatttgc cctacaccga
    14881 catccacatc ggcctgatcg tcggggtgat cgcgatcgtg atcgtctggc tgctcttccg
    14941 tgagaccgtg ccggggacga tgctggatgt cctcggccgg aaccggcggg ccgcgaagca
    15001 tgccggtttg cccgtaaccc ggctgattgt cggcgcgttc ctcgtctccg gggcggcggc
    15061 gggcctggcc ggggcagtcg acgtgctcgg ggttttcggt ctcttcaagg cgacctacca
    15121 gccgggctac ggcttcatcg ccttcgcgct ggcctacctt gcccggctgc gatcgatcgc
    15181 ggtgatcccc ttcgccgcct tcctgtcggt gcttgtgatc ggcggcgatg cgatgagccg
    15241 ccgggccgat gtgccgtccg actttgtccc cgtgctcgag ggactgatgc tgctcttctt
    15301 cgcgctggcg gtctggttcg agggacgagg gagcgcggca tgaccatcaa cggcgtctct
    15361 ctcgtcgatt tcgcggcggt cctgctcgcg actggcctgc tccgggcggt gccgctggtc
    15421 gtggccggga tcggcgaagc ggtcgccgag cgggccggac tgctcaacct cggcatcgag
    15481 gggatgatgc tgagcggctg cttcttcggc ttctgggcgg cgtacgagac cgacagcctg
    15541 ctgatcggga tgctggcggc ggtcgggtca gggctcgtgc tggcgctgct cttcggctgg
    15601 ctggcgatca cgctcaagat cgaccaaatc ctgatcggtc tcgccatcac gatcgccggg
    15661 agtgggctga ccggctatct cttccgggac cagttcggcg gtcgaaatgt ggcggtcgat
    15721 gtcgacacca gcaagatcca cgtgccctgg ctgaaggaga tcccggtgct cggcccggcc
    15781 ctgttcgagc agcagccgct cttctacgtg agctgggcgc tggtcccgat cgtggcctgg
    15841 gtgctgttcc ggactcgctt cggtctgcag gtgcgcggcg caggcgagac gccggtggcg
    15901 gtcgatgcgg ccggggtctc ggtcgagcgg gtgcggtacg tggcgatcgc gatcggcggg
    15961 gcgctagccg gattcgcggg tggcttcctg tgcgtggccg atgtcggaat cttcaccacc
    16021 aacatgaccg tcgggcaggg cttcatcgcg ctggccctgg cgatggtcgg gaactggagc
    16081 ccgtggcgga tcgcggttgg ggcgatcgtc ttcggcgtgc tgcgctcgat gggcgacggg
    16141 ttgcagatcc tgggggtgaa cacgcgaccc gagttcctgg cgatgctgcc ttacctcggc
    16201 gtgatcgtcg ccatggtcct cctcgccggc cgcacaagac tcccctccgc gttagccacg
    16261 ccgtaccgga gaggcatgcg gtgatggcga ggaaatgtag cgtccgctaa cggctcagct
    16321 cctaggacca aactgctata tggtcgcttt gtattccgca tgataggatc gccaaaagta
    16381 ccgtccaagt gaaattcggc cctgcaaaac gacggggctc gcggagcggt cggacatacc
    16441 ggggcgaaag tcgcctcggt atctgcgtat ctggtgtatc gccgacgccg ggtgtcgact
    16501 tgagggagat gatcgtgcgg agactcgttc ttgggatcct gcttgttttg cctctgcttg
    16561 ccggtgttct gcccgcatcg gcccaggatg cgcgctacga gatcaaggat ctgatcaagg
    16621 cccacgacat cgggaacgag gcagccggcc tcggcgcgta cgacatcaac gacgacggta
    16681 tgatcgtcgg tttcactgct caatccgcca ccaaatccag tcccttctac atcgtcaacg
    16741 gcaagacaac ccggatcaag accggtaagt tcggagccac ctatggacat gtcaacgccg
    16801 acggggtgat cgtcggtcgc gacgtcaccg gtcgcgatga gaacggcgga ccgatcggct
    16861 taccggcatt ctgggccgat ggcaaaacga cccggctaga gctgccgctc gatccgctcg
    16921 ggcaaacagt cgtcaccggt gtcgcccagg agatcaacga cgccgggcaa atcgtgggcg
    16981 aagtccagta tccgggcgac gacttcaacc agtacgcggt gctctggcag gacggtgtgc
    17041 cgaccctgct gcccgatgcc ttcggttacc cggtctgctc cgcctggggc ctgaccgcat
    17101 ccgggctggt cgccgggtcg tgctgggagc cgaacctggg catgtttctg ccggtcctct
    17161 ggcaatcact ggtgccaatc tcgctcgatt cccaggcgac cgccaacacg gatgtgacgg
    17221 ccgccacgga caccgcggcg gggggttatg ccgtcgtcgg gtaccaatac gatgacgtgg
    17281 ataacccctc ctacctcgag gccaccgtca tcatcgatgg cgttatccag tccctgcccg
    17341 gcccggccgg atataccgcc tgccgcgcga tgacggtcag ccagacagag gccggattgc
    17401 tggtgggcgg atcctgcgcg ccggacgtcg ccactggcca gaagagttgg gccggcagtg
    17461 tggcggtggc gtggctggac ggtgaactga tcgagcttaa cgacgccatt ccgcaggatc
    17521 agggctggac cctgatcctg atccgcggtg tcaacggcaa gggtcaaatg gttggactgg
    17581 gtgagcggaa gggagagcgg aggtcattcg tgctgacacc aactgagtag cagcaccgtc
    17641 gttcttgaaa tgatcaattg gacttggaga aacgattcag atgggaatta cacggcgact
    17701 cttcctcggt ggtggtgcgt cgcttgcggc ggcgatggct tcgggcacga gattgaaacg
    17761 gcgctcagca agggcacagg cgagtgcctg ggaggttgtc gatctttttg cgttgaccga
    17821 ggcgctttct tatcgcggta acgagtatgt gggctccttc ctggatatga acgccgcggg
    17881 ggaggtcggt ggctccttcg tcgtctccga cgtcaagatt tcgccggcaa tctggtctcc
    17941 tgatggcaag gcgacgcgcc tcaagagcgg taagtacggc ggcgaagtgt gggcgcttaa
    18001 caacaatgga ctcgcagtcg gcgccggctt tcttggcgta cgcggcttcg agtcagagga
    18061 atcgttttac cctggttact tgaccccacg ggcctggaaa gatggtcaac cggtcgagct
    18121 ggaggtcctg ggaggtgaca taaaggtatc gaccggcatc gttcaggacc tcaacgacga
    18181 cgggatcatg gtcgggtatc tcgtcgatcc tgcgcttaac gtggaagtgc cggttaaatg
    18241 gcaggatggc gcggcgacgg tgctggccgg tgggagtccg ggcggtggct ttgccaacca
    18301 gatcaacagc gccggtgaca tcggcgggta cgccagaatc gacggcacca gcacggccgt
    18361 catgtggcgg ggcgacaaga tggacgtgct ggcgcccccg gacgaccccg acctggggga
    18421 gaactccgtc ttcctcctgg gcgacctcgg cgacaatggc cagatccttg cggttatgaa
    18481 cgtcccgtat gagatcgatg cgtccaccat cgggtactcc aatgcgtcct acgtgctcga
    18541 caagggtaag tggaccaagc tggccagccc ggacgagaca cgccagagcg tgtacggccg
    18601 ctcgatgaat gcgagtggcg tcgttgtggg gtcgatcaac tcgaccgagg aggacgtcca
    18661 ggccctcagc gcgatctggg agggcggcga ggggcgcatt ctcgacgaag aagtggaact
    18721 gccgagcggg ctgaagatgg cgggcgtcgt caagatcaac gacgagggcc agattcttgc
    18781 ggacgccagg gacgccgatg ggacacgcca cgccgtcatc ttgcggccag cgtagaccaa
    18841 gcattgttcg gaccgcgcca ctctgcattg caagggaagc aggtggagag tcgacggaac
    18901 gggacagaca gtaacgcagc ccgggtcttc aggcccgggc tgaaccatga acccggctga
    18961 agccggctgg gacggcgggc aataatcaat gagatttcag ataatgccgg tatgtcgtat
    19021 tggcgcttgc actatcacct catttgggcg accaaacagc gccttcccct cattggttcg
    19081 gaggaagagg cggtcatccg cgagtccctg gaaatgacca tggcacgcat gaagttgatc
    19141 ggtcatgcga taggactcgt ggcagaccac gtgcatatcg cgctttccat accacccact
    19201 gtttccgtcg ccgacgccat ggggcggcta aaaggcggat catcacatcg tgttggccag
    19261 cgcctgccta aagcaactgg atttgcctgg caagcggaat acggcgccct cagcctcacc
    19321 gaacgtgcct tgccgaccgt gatcgagtac gtgcagaacc agaaacagca ccacgccgcg
    19381 aacacattga tctatggttt cgagcgcatc gaaagccaag ctccaagatc tagagccata
    19441 ccgcaaggcg ccgactgagg agccggcttt agccgggttc aggtttgagc ccgggtcttc
    19501 aggcccgggc aatcccgcca atctccactc gactcacgca tattccctgc tcacgactca
    19561 cgtcccatga ctcgctcaaa tctcgctcta atccggggct gccagagtga ctttcagtcc
    19621 ggcaaatgag tagccggacg cccggtttcg aacgggtcct ccgcctggct ggcgcgaccc
    19681 gttccgcgtg tttggagtgg ttgtcgtatg gaaatgagag gcgtgaatcc gaccctcaag
    19741 atggctggca tggtgtcggt ggccctggtc gggctgctgc acctgattga ggcgccggag
    19801 tattacggcg aggtcaagta catcggcgcg ctgttcgtgc tctgcgcggt tggcgcggcg
    19861 gtctccgtgt tcggcatctg gagggacctg cgcgcgggct ggctgctcgg cgcggtcctc
    19921 gccggcagca acttcatcgc ttatctcctc agccgcaccg tcggcctgcc gagcttccgc
    19981 gagaactcgt ggtctcagtt cgccgagccg atggggctgc tctccctcgc cgtcgaggct
    20041 gtcgccttca tcatctgcat ccgtgccctc cgtgccgccg gcgcggagaa caacggccgg
    20101 atcgcccttg cctgagccaa gcccttcctt ccccttccca acgcacaccg atgccgcgcc
    20161 cgatccgcgc ggcatcggcg tatgcttgcc catggcagct ctatccctga gacgttctac
    20221 atgcggatcc tgatcatcga agacgaagtg cggctggccg ggatcatcgc ccggatcctg
    20281 acccaggagc gattcacggt cgatgtcatc tccgatggcg aagagggcct gcacgccgcc
    20341 ctgaccgacg cctacgacgc gatcatcctc gaccgcatgc tgccccggct cgacggtgcc
    20401 agcatcgtcc gagagctccg cgccgagggc aacgccacgc ccgtcctgat gctgactgcg
    20461 ctcggcgacc tgccggaccg ggtcgaaggg ctgaacgccg gcgccgacga ttacctcggc
    20521 aagccgttcg cctttgagga gctgctcgcc cgcctgcgcg ctctggtccg ccggaccgat
    20581 cgcccgctcg cgcccgaccg ggtcgaggtc ggcccgatcg cgatcgacct gcgcggccgg
    20641 accgtggagc ggaacgggaa gccggtcgat ctctcggcgc gggagtacgc cctgctcgag
    20701 ctgatggccc gtaacagggg ccaagtcctg agcaaggaca cgatcctcga gcgcgtctgg
    20761 ggaatcgatg ccgagccgca gggcaacgtg gtcgagctct acgtcttcta cctgcgccgc
    20821 aagctgggcc acaccgccgc gcatcctgtc atccgcacgg tcaggggagc cggctatgtc
    20881 cttggctagg gccaatacga gcgtcttcgg ctacatcggc cggcggatgg cactggtcaa
    20941 cgcggccgtc gtcctgctcc tgatcgcggt cgtcggcttc gggatggtgg cggtggcacg
    21001 ctacgtgatg attcagcagg cggatcaggc actcaaggat caggccaatg tcgccgccct
    21061 cgattggggc ccgaatctcg ccaccggcca gccgattccg gttgcggacc ggccgatcga
    21121 cggtgacggg gatgggcacg atggcgacga tgagctggag gccgaggagc tgctcgatag
    21181 cggcgatctg atcgcgttcg gcttcgacgc ccagggcacg ctggtcgcca acccgcgcgg
    21241 gatgcggatg accgagttgc ccgagcagca ggcagttgcg ggagccctga ccggccacga
    21301 cgggtaccag accgtcagcc tgcacgatga gccggatgtc cgcgtctaca cgatgccgat
    21361 cgaggtcgat caccaggtgg tcggcgcggt ccagatcgtg cgcaaccaga aggatctgcg
    21421 ctcagcgatc cgggcgatcg agttggcggc actggtcgcg gcggcgatcg gcgcactcgg
    21481 ggcggttccg accgggctgt acctggcgaa gcgggcgatg cgaccgatcg accgggcctt
    21541 ccgtcgccag caggccttca tcgccgacgc ttcgcacgag ttgcgcacgc cgctggcggt
    21601 cgtccgggcc aacaccgagc tggtccggcg gatgcccgac gcgacgccgc aggagcggga
    21661 cgtcgagttg gccgcgatcc tcgaggagac ggaccggatg gcccggctgg tcgatgagct
    21721 gctggtgctg gcgcggtccg atgccggccg gctggagctg aagtcggagg tgctcgatct
    21781 ctcggcgctg gtgcgggagg cggcagagcc gatgcgcgcg ctggcggccg aggccggcca
    21841 aacgctcgag atcgacgcgc cggacgagct cctgatcagc ggcgacgaag accgcctggc
    21901 ccagatcctg cgcatcctca tcggcaacgc gatcgcctac acgccggcgg gcggaacgat
    21961 cactgtgaag tcgtcagacg tcagacatca gacgccagtt gagacggcaa agaaactcac
    22021 gattcacgac tcacgactga cgactgcgac cctcaccgtc gccgataccg gcatcggaat
    22081 gagcgcggag gagcgggcgc gggtcttcga ccggttctat cgtggcgagc aatcgcgcag
    22141 ccggggcacg ggcggcgtcg ggctcggcct ctcgatcgcg cagaccatcg tc
//
LOCUS       DATAOZ010000261         9895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_112356, whole genome shotgun sequence.
ACCESSION   DATAOZ010000261 DATAOZ010000000
VERSION     DATAOZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112356"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..686)
                     /locus_tag="VHR64_06900"
     CDS             complement(282..686)
                     /locus_tag="VHR64_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3302594.1"
                     /translation="MQITRSSVATMAGPADWFTGTVFVDSVASPTGIARVSASNVHFA
                     PGARTAWHTHPNGQTIFVTEGVGRCQRRGGPVEVIRPGDRVFFEPGEDHWHGAAPDRF
                     MTHLAMQEADDSGNVVTWGEHVTDDEYSRPAD"
     gene            complement(768..1139)
                     /locus_tag="VHR64_06905"
     CDS             complement(768..1139)
                     /locus_tag="VHR64_06905"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HEX3302595.1"
                     /translation="MNTWTGNEFDKIASAEELQIASVRRDGTLRSPVTIWVVRHGDDL
                     YVRAVNGRSKGWFRGTQDRHEAHVRAGGVDKDVQLVETDDLIDEIDAAYRTKYNRYPA
                     NIIGGVLTPQARAATLRLVPR"
     gene            complement(1294..3057)
                     /gene="ilvD"
                     /locus_tag="VHR64_06910"
     CDS             complement(1294..3057)
                     /gene="ilvD"
                     /locus_tag="VHR64_06910"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX3302596.1"
                     /translation="MATTNGKGANGNGAFDVRHMSRNLLDGVERAGARAMLKGIGLTD
                     EDLQKPMILIANTWIETMPCNLGLRTLAHAVKEGVKAAGGTPMEMNTIAISDGVTMGT
                     EGMKTSLISRELIADTVELVGRGHMFDAVIGLGACDKTLPGLSLALARLNIPGFLLYG
                     GTILPGEVNGQEATVRSVYEKIGAYKAGKISEEELKLFEDHACPTHGACGGQYTANTM
                     AMAMEFIGLSPLGTASPPAVSDEQWHEGIGTRGDVVNPRKMEIGRQAGRLIMDMLKQG
                     IRPLDILTREAFENAIAGVAATGGSTNSVLHLLALAREAKVELSIDDFDAISRRTPLI
                     ADMMPGGKYAAADLDKAGGTPLIAKRMVEGGYLSGDQISPSGQTIATEAAVAVETPGQ
                     DVVHTLDNPINEHGGLIILKGNIAPDGGVIKLFGYERHYHKGPARVFDSEHEALEAVY
                     ANKINPGDVVVIRYEGPKGGPGMQEMLAITAALIGQGLGGECALVTDGRFSGATKGLM
                     CGHVAPEAAVGGPIAAVHEGDTVVMDIDNRRLDIEVPDDEITRRLQSWTPPAPRYTSG
                     VMAKYAAMVRQANEGAITLPN"
     gene            3167..4045
                     /locus_tag="VHR64_06915"
     CDS             3167..4045
                     /locus_tag="VHR64_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3302597.1"
                     /translation="MAGLTFGIQTPPFHDWPRMIADWQRFEALGWDSLWLPDHFVPTF
                     RRDLPFFEAWTLLSALAMTTDRVRIGVLVSCNTFRHPALLAKQAATVDHISHGRLEVG
                     LGTGWVEFEHTMFGIEYPDNAERVDRFGEAVQVLDLLLRNEVSSFVGEHYRLDGATFR
                     PAPIQKPRPPLTLAAHGPKMLRLIAPYVDRWNSMGSPAEMGERGQRLDEALVAAGRDP
                     ESVVKSVLYVPSVTPDEDPWASVDAFEDFVSRYRKQGVRDFIFQPAHAPLDLIERVSH
                     EVLPSLRKHLDILPPQ"
     gene            4079..4339
                     /locus_tag="VHR64_06920"
     CDS             4079..4339
                     /locus_tag="VHR64_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX3302598.1"
                     /translation="MTDLASILNLLFTVLSLAVIARALLSWFDPTMRYPISRMIVDLT
                     EPIIAPIRRVVPPVGGMIDLSPLIALILLQVIERVIINSLHT"
     gene            4436..4558
                     /locus_tag="VHR64_06925"
     CDS             4436..4558
                     /locus_tag="VHR64_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302599.1"
                     /translation="MGNVIRFVSMAGFAILILLIILTIVSIFGGFEHPLSAPPT"
     gene            complement(4555..6375)
                     /locus_tag="VHR64_06930"
     CDS             complement(4555..6375)
                     /locus_tag="VHR64_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302600.1"
                     /translation="MTNSPETQTALEARLHQRIQAAAATRDAITVRWNQVSNLRLILA
                     ALVIGFGFWWLRTGEGTWGLIAGGLALVFVAALIWHRRLGAMRADAATRVRLRQQAIA
                     RIHRDWRSLPEPLAQEVALDHPYAHDLDLVGHGSLQQLIDTTQTPAGRRTLLHWLTNG
                     APEGEINARQHIATDFARDEQWRENLATSGSRTGEPGGDVELLLAWLETAATERIGAR
                     HLIALLLAIGTVAVGVLYVLGVASSAWLLPFIVANAILTFVAPNAGQLSQINSHQRSL
                     SQYRAVLPVAEAAPGSSAETDRVRGRLTAPGGSASHQLAALDRALAFVIPAGTIIWFP
                     LQLALNWDLLVEARLRRLAASIGPHLRGWIDAIGEIEALSSLGNASALNPDWTWPSVK
                     ADANAFAGSALGHPLIPATRRVANDVTVGPGGSVLIVTGSNMAGKSTLLRTVGLNAVL
                     ARAGGPVCAAGLRMPNYPIWSSVRIQDSLEEGVSLYMAELLRLKQIVDAARSGPILYL
                     LDEILHGTNTTERRIAARSVIRQLVRTGSIGAVSTHDLELIDTGLADASILVHLVDQV
                     VDGPNGPEMMFDYRLRPGLAPSSNALRLLNLVGLGEESPV"
     gene            complement(6455..7291)
                     /locus_tag="VHR64_06935"
     CDS             complement(6455..7291)
                     /locus_tag="VHR64_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX3302601.1"
                     /translation="MSTNSLAANRDEVAVFVDLENIRYSTINSFGREPDPLVWRDKAL
                     QYGLMAVARAYADFDQHPAAIRTRLEVAGFEAQHFAAKRATDSQGRERVVSRSDLNFA
                     VDIINTALARPDIETFLLFTGDKDFIRLVTTLRNRLGRRVVICGIPGSISPDLVTAAG
                     EEDQIILEPCIDNDIAVIRAIHTYIGQLHEGFVPTQSHMSRTLWRFLDKSALPSEHIE
                     AKIMEFVRKGILSKRQTVNGQNQELVTTELNPTNALVVEALTDWDPEPANGRCDDLDE
                     TE"
     gene            complement(7484..8476)
                     /locus_tag="VHR64_06940"
     CDS             complement(7484..8476)
                     /locus_tag="VHR64_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX3302602.1"
                     /translation="MSIALGIDFGGTKVLAGVVNLDTGEVLGTAKKKTDARDSADQMM
                     SRLYDVVESASQKAGVPIKDLAGIGVGLAGQVDTERGILLGAPNLSQSTVDLPMADLL
                     TKRFKIPARLRNDVQVAAIGEAAFGAGRDQPDFVCIFVGTGIGGAIVRGGRLVTGAAG
                     AAGEIGHIVIHANGRICGCGGRGHLEAYASRTAMTQALLGEMKRGRPSMLIDAVNESD
                     DAPGGTAIRSGQLATAVDAGDELALDIVNEAGRYLGYGLASAINLLNPSRIILGGGVI
                     EAVGLLFHVAERTARREALATSAQSVGIVPAALGDNAGVVGAAMLHAPNSIVTV"
     gene            8556..9419
                     /gene="dapF"
                     /locus_tag="VHR64_06945"
     CDS             8556..9419
                     /gene="dapF"
                     /locus_tag="VHR64_06945"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX3302603.1"
                     /translation="MTGRTIPFTKMSGSGNDFVVIDNRSGVLDRLDIPAFTRAVSRHR
                     ISVGADGVVMLSEPPEGDRTLSYAWRYVNADGSDGEMCGNGAMCAARFAVRTGIAEPH
                     HWFLTPSGIVEAWAEAASPSAAIAIADPERIEEPVELSVLGRTLCCVRVVVGVPHVVI
                     RVDDADAFADAGTFHAIGSALRLHDVFAPAGTNANVVSRIDDETWRMRTYERGVEAET
                     LACGTGAVASAIVLGHQGLAQSPTRIRTSSGRDLTVSYDLNDSQATHVRLAGHAAIIY
                     DGTLTEEAFQG"
     gene            complement(9607..>9895)
                     /locus_tag="VHR64_06950"
     CDS             complement(9607..>9895)
                     /locus_tag="VHR64_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3302604.1"
                     /translation="RALPHSQPIRPANDIVAYFGKVVSSYPSAHWEVSDVIASGDRAA
                     VQFVVREFSTRLGRDLLSEQVVVMRAAQGRIVNIVGYYDSNEFRRVFWEES"
BASE COUNT         1681 a         3214 c         3195 g         1805 t
ORIGIN      
        1 cgataggccg ctggcggcga caaataatcc gcgctgaggc cggggccaga gcacgtttgg
       61 aagagccgcc tcgtatctaa cgcagcgcgg gggtgccctc tttccttgag gagagagccg
      121 gagagaggag catgcgccgc ccggccccga cgacgttcga atcgttgcga gaaccgccct
      181 tgtgccggtt cggctccatg aattgcgcgt ggggatgcat gaagccgggc gtcgtaaccc
      241 cgcgcttcgt caacgcccgg gcaacgcttc tgagctccgt ttcagtcggc gggccggctg
      301 tactcgtcgt ctgtgacgtg ctcgccccag gtcacgacgt tgccggagtc atcagcctct
      361 tgcatcgcga gatgggtcat gaagcggtcc ggggcagcgc cgtgccagtg gtcttcccct
      421 ggctcgaaga agacgcggtc gccgggtcgt attacttcga ccggtccgcc gcgacgttga
      481 cagcggccga cgccctcggt gacgaagatc gtctgaccgt tgggatgggt gtgccaagcc
      541 gtgcgggcgc ccggagcgaa atggacgttg cttgcactca cccgcgcgat gccggtcggc
      601 gacgccaccg agtcaacaaa gaccgtgccg gtgaaccaat cggccggccc ggccatggtc
      661 gcgacagagc tcctggtgat ctgcatgaga tgatgcctcc ggttgacgtt ctctgggcgc
      721 gcgtatccca atctgcaacc gtccgggtgc aattggacgc aggttcgtca gcgcggtacg
      781 agcctaaggg tcgctgctcg tgcttgcgga gtcaggaccc cgccaatgat gttggcaggg
      841 taccggttgt acttcgtacg gtatgcggcg tcgatctcat cgatcaggtc gtccgtctcg
      901 acaagctgga cgtccttgtc gacaccgccg gcgcggacgt gcgcctcgtg ccgatcctgg
      961 gtgcctcgga accagccttt cgaccgaccg ttcacagccc ggacgtagag atcatcgccg
     1021 tggcggacaa cccatatcgt caccgggctg cgcagcgttc cgtcccgtct gacggatgca
     1081 atctgaagct cctcagcaga cgcaatcttg tcgaactcgt tgcccgtcca ggtgttcatt
     1141 cgggtacctc cttcgctgct ggaggtgagt ctgaactgga ctacaaaccg agcaccgagc
     1201 ggatgctgca catatcggtc cgcggtgctc aatccgtgcg agcaatggaa ccggcgcccg
     1261 gttcaatacc cgggcgccgg tcgcgactca tcgctagttg gggagggtga tggcgccttc
     1321 gttggcctgg cggaccatcg cggcgtactt ggccatcaca ccgctggtgt agcgcggcgc
     1381 cgggggcgtc caggactgca gtcgtctggt gatctcatcg tccggaacct cgatgtcgag
     1441 ccgacggttg tcgatgtcca tgacgaccgt gtcgccctcg tgaactgcgg cgatcgggcc
     1501 accgacggcc gcctccggtg cgacgtgccc gcacatcagc cccttggtcg cgccgctgaa
     1561 ccggccgtcc gtgaccagcg cgcactcgcc gccgaggccc tggccgatca gcgcggcggt
     1621 gatcgccagc atctcctgca tgccggggcc gcccttcggt ccctcgtacc ggatgacgac
     1681 gacatcgccc gggttgatct tgttggcata gaccgcttcg agcgcctcat gctcggagtc
     1741 gaaaacccgg gccggaccct tgtggtaatg gcgctcgtag ccgaagagct tgatcacacc
     1801 gccgtccggc gcaatgttgc ccttgaggat aatcaggccg ccatgctcgt tgatcgggtt
     1861 gtcgagcgtg tgcacgacat cctgacccgg cgtttcgacc gcgacggccg cttcggttgc
     1921 gatcgtctgg ccgctcgggc ttatctggtc gccgctcagg tagccgccct cgaccatccg
     1981 cttggcgatc agcggggtgc cgccggcctt gtcgagatcg gcggccgcat acttgccgcc
     2041 cggcatcatg tcggcgatca gcggggtgcg ccgggagatc gcgtcgaagt cgtcgatcga
     2101 gagctcgacc ttcgcctcgc gggccagcgc cagcagatgc aagaccgagt tggtcgatcc
     2161 acccgtcgcg gcgacgccgg cgatcgcatt ctcgaaggcc tcgcgggtga ggatgtcgag
     2221 cgggcgaatg ccctgcttga gcatgtccat gatcaggcga ccggcctgcc ggccgatctc
     2281 catcttgcgc gggttgacca cgtcgccccg ggtgccgatg ccctcgtgcc actgctcgtc
     2341 gctgacggcc ggcgggctgg ccgtgccgag gggcgagagg ccgatgaact ccatcgccat
     2401 cgccatggtg ttggccgtgt actgaccgcc gcaggcgccg tgggtcgggc aggcgtgatc
     2461 ctcgaagagc ttgagctcct cttcgctgat cttgccggcc ttgtacgcgc cgatcttctc
     2521 gtagaccgag cggaccgtcg cttcctgccc gttgacctcg cccggcagga tcgtgccgcc
     2581 gtagagcagg aacccgggga tattcaggcg agccagcgcg agagagagac cgggcagcgt
     2641 cttgtcgcag gcgccgagac cgatcaccgc gtcgaacatg tgaccgcgac cgaccagctc
     2701 gaccgtgtcg gcgatcagct cgcgggagat gagcgaggtc ttcataccct cggtgcccat
     2761 cgtcacgccg tctgagatgg cgatcgtgtt catctccatc ggagtgccgc cggccgcctt
     2821 gacaccctcc tttacagcgt gcgcgagcgt gcgcagtccg aggttgcagg gcatcgtctc
     2881 gatccacgtg ttggcgatca ggatcatcgg cttctggagg tcttcgtcgg tcaggccgat
     2941 acccttcagc atcgcccggg cgcctgctcg ctcgacgccg tcgagcaggt tccggctcat
     3001 gtgccggaca tcgaatgcgc cgttgccgtt tgcgcccttg ccgttcgtgg ttgccatggc
     3061 ggtgcctcgt gtctcccctg aattggcgca caatgccggt gcgcgctgga atgcgttggc
     3121 gctagtttcg catagcaggc agaaccgcag gaaaggacca cccgggatgg cagggctcac
     3181 gttcggcatc cagactccgc cattccacga ttggccgcgc atgattgccg attggcaacg
     3241 gttcgaagcg ctcggctggg actcgctctg gctgccggat cacttcgtgc cgacatttcg
     3301 ccgcgatctc ccgttcttcg aggcatggac gctgctctct gctctggcga tgaccacgga
     3361 ccgcgtgcgg atcggcgtgc tggtgagctg caacaccttc cggcaccccg ccctcctggc
     3421 caagcaggcg gcgaccgttg accacatcag tcacggccgg ctcgaggttg ggctcggtac
     3481 cggctgggtc gagttcgagc acacgatgtt cgggatcgag tacccggaca acgccgagcg
     3541 cgtcgaccga ttcggcgagg cggtgcaagt gctcgatctc ctgctgcgga acgaagtcag
     3601 cagcttcgtg ggcgagcatt accggttgga cggcgcgaca tttcgcccgg cgccgattca
     3661 gaaaccgcgc ccgcccctga ctctggccgc gcacggaccg aagatgctgc gcttgattgc
     3721 tccgtacgtc gatcgctgga actcgatggg ctcaccggcc gagatgggcg aacgcggcca
     3781 gcggctcgat gaagcgctgg tcgcggcggg acgcgatcct gaatcggttg tcaaatcggt
     3841 cctctacgtg ccgagcgtga caccagacga ggatccgtgg gcatcggtcg acgccttcga
     3901 ggacttcgtc agtcggtacc ggaagcaggg cgtccgcgat ttcatcttcc agccggccca
     3961 tgccccgctg gatctaatcg agcgcgtatc tcatgaggtc ctgcccagcc tgcgaaagca
     4021 cttggatata ctgcctcccc agtgatgcgg ccgtgggcgc cgcactggag cgcaatctgt
     4081 gaccgatctt gccagcatcc tcaaccttct cttcactgtc ctctcgctgg ccgtgatcgc
     4141 ccgcgcgttg ctctcctggt tcgacccgac gatgcggtat ccgatcagcc ggatgatcgt
     4201 ggacctgacc gagccgatca tcgcgccgat ccgccgggtc gtgcctccgg tgggcgggat
     4261 gatcgatctc tcgcccctga tcgcgcttat cctgctgcag gtgattgagc gcgtgatcat
     4321 caattcgctg catacgtagc gttgtgtacg ggacgggctg ctgcctggtc ccgtagaatg
     4381 gcgccagtgg gagcctccgg cactcggccg gaatcagaca gatggagcgg cagcaatggg
     4441 taacgtgatc agatttgtca gtatggcggg gttcgcaata ctgattttgc tgattattct
     4501 gaccatcgtc tcgatcttcg gcggcttcga gcacccgctt tccgccccgc cgacctagac
     4561 cggcgactct tctcccaggc caaccagatt gagcagcctg agcgcgttcg acgacggcgc
     4621 caggcccggc cgtagtcgat agtcaaacat catctccgga ccattcggac cgtccaccac
     4681 ctgatcaacg aggtggacca ggatcgacgc gtccgccagg ccggtatcga tcagctccag
     4741 gtcatgcgtc gagaccgcgc cgatcgagcc ggttcggaca agctgtcgaa tgactgaccg
     4801 ggcggcgatc cggcgctcgg tcgtgttcgt gccgtgcagg atttcgtcga gcaggtagag
     4861 gatcggcccc gaccgcgcgg cgtcgacgat ctgcttcagc cgcagcagct ccgccatgta
     4921 gagcgagacg ccctcttcca gcgagtcctg tatccgcacg ctcgaccaga tcgggtagtt
     4981 cggcatccgc agaccggccg cgcagaccgg tccaccggcg cgcgccagca ccgcattgag
     5041 accgaccgtc cggaggagcg tgcttttgcc ggccatgttc gagccggtca cgatcagcac
     5101 cgacccgccc ggaccaacgg ttacgtcgtt ggcgacacgg cgcgttgcgg ggatcagcgg
     5161 atggccgagc gcactccccg cgaacgcatt ggcgtcggct ttcacggacg gccacgtcca
     5221 gtccgggttg agggcggacg cgttgcccag ggacgaaagc gcctctattt cgccaattgc
     5281 atcgatccag ccgcgcaggt gcgggccgat cgaagcggcc agtcgacgca ggcgcgcctc
     5341 gaccagcaga tcccagttaa gggcgagttg aagcgggaac cagatgatcg ttccggccgg
     5401 tatcacgaac gcaagcgccc gatccaatgc agccaactgg tgcgaggccg agccaccggg
     5461 agcggtcagt cgaccccgca ctcggtcggt ctccgctgaa gagccgggcg cggcttcagc
     5521 gaccggcagg acggcccggt actgggagag cgagcgctga tgactgttga tctgcgaaag
     5581 ctgccccgcg tttggcgcga cgaatgtcag tatcgcgttg gcgacgatga acggcagcag
     5641 ccaggcagat gaagcgacac ccagcacgta aagcacgccg actgccaccg tgccaattgc
     5701 gagcaggagg gcgatcaggt gccgcgcgcc gatccgctca gttgccgctg tctcgagcca
     5761 ggccaggagc agctcgacgt cgccgcctgg ctcgccggtg cgtgatccgg acgtcgccag
     5821 attctcgcgc cactgctcgt cgcgggcgaa atcggtcgcg atgtgttggc gcgcgttgat
     5881 ctctccctcg ggcgcgccat tcgtcaacca gtggagcagc gttctcctcc cggctggggt
     5941 ctgcgtggtg tcgatgagtt gttggagcga gccgtgaccg accaggtcca ggtcgtgcgc
     6001 gtagggatgg tcgagcgcga cctcctgcgc caacggctcg ggcagcgacc gccaatcccg
     6061 gtgaatccgc gcgatcgcct gctgccggag ccgaacccgg gttgccgcat ccgcgcgcat
     6121 cgcgccaagc cgtcgatgcc agatcagggc tgcaacgaag accagcgcga gcccacccgc
     6181 gatcaggccc caggtgcctt cgccggtgcg tagccaccag aagccaaagc cgatcaccag
     6241 tgcggccagg atcagccgca gattggagac ctggttccag cgaacggtga tggcgtcacg
     6301 cgtcgccgcg gccgcctgga tgcgctggtg caggcgcgcc tcgagcgcag tctgggtttc
     6361 gggggaattc gtcaatcgtc cgccgtccgg ttcgggccaa tgttggctcg attctgacac
     6421 accggttcgg ctcgatggtg tgagtggggc agggttactc agtctcgtcg aggtcgtcgc
     6481 accggccgtt ggccggttcg ggatcccaat cagtgagcgc ctcgacgacg agcgcgttgg
     6541 tcgggttcag ctcggtcgtg accagctcct gattctggcc gttgaccgtt tgccgcttgc
     6601 tgaggatgcc cttgcgcacg aactccatga tcttggcctc gatgtgctcc gaaggcagag
     6661 cgctcttgtc gagaaaccgc cagagggtgc ggctcatgtg gctctgcgtc gggacgaagc
     6721 cctcatggag ctggccgatg tacgtgtgga tcgcgcggat gaccgcgatg tcgttatcga
     6781 tgcacggctc gaggatgatc tggtcctctt cgcccgccgc cgtgacgagg tcagggctga
     6841 tcgagcccgg gataccgcaa atgacgactc gccggccgag ccggttgcga agcgtcgtca
     6901 cgaggcggat gaagtccttg tcgccggtga agagcagaaa cgtctcgata tcgggacggg
     6961 ccagcgcggt gttgatgatg tcgaccgcga aattgagatc ggaccgggag accacgcgct
     7021 cgcggccctg gctgtcggtc gcgcgcttgg cggcgaagtg ctgcgcctcg aacccggcaa
     7081 cttcgagacg ggttcggatc gcggccgggt gttgatcgaa gtccgcatac gctcgggcga
     7141 cggccatcaa gccgtactgg agtgccttgt cgcgccaaac cagcgggtcg ggctcgcgtc
     7201 cgaatgagtt gatggtggag tatcgaatat tctcgagatc tacgaaaacc gcgacctcgt
     7261 cgcgattcgc cgcgagacta ttagtgctca tcgatagttc cgatctgact ctcttacgtc
     7321 aatcgatcgc gtctcatcca aagcgattac gaggatgacg agatgtatac ccagcgggat
     7381 cccagacaat atcgggcacc cgaattgcac accaagagta gcgcagccga taacgtggcg
     7441 tgcgcaacat tactgttcga tagtatcacg tccaccccgg accctaaaca gtgacgatgg
     7501 agttgggcgc gtggagcatt gcggcgccga cgactcccgc attgtccccg agggcagcgg
     7561 gtacaatccc aactgattgg gccgacgtcg cgagcgcctc tcgtctcgca gtacgctcgg
     7621 caacatggaa aagcaacccg actgcctcga tcacaccacc cccgaggatg atccgggacg
     7681 ggttgagcag attgatcgcc gaggcgaggc catagccgag atatcgcccg gcctcgttga
     7741 cgatatcgag cgccaactcg tccccggcgt cgaccgcagt tgccaactgg cccgaccgaa
     7801 ttgccgtacc gccgggggca tcatccgatt cattgacggc gtctatcagc atgcttggac
     7861 gcccgcgttt catctcgccg agcagcgcct gggtcatcgc cgtgcgcgat gcatacgcct
     7921 caaggtgacc gcgaccgcca cagccgcata tccgcccgtt ggcgtggatc acgatgtggc
     7981 cgatctcacc ggctgcgccc gccgcaccgg tcaccagccg tccgccccgc acgatggcgc
     8041 caccaatccc ggtgccgaca aagatgcaaa cgaaatccgg ttgatcgcgg ccagctccga
     8101 acgccgcttc gccaatcgcc gcgacctgaa catcgttccg gagcctggcc ggtatcttga
     8161 accgtttggt cagtaagtcg gccattggca aatcgaccgt cgactggctc agattcggcg
     8221 ctcccagcaa gatcccgcgc tcggtatcga cctgaccggc gagtccgacg ccgatgccgg
     8281 ccagatcctt gatgggaact cctgccttct ggctcgctga ttccacgaca tcgtagagcc
     8341 ggctcatcat ttggtcagcc gaatcccgcg cgtcggtctt cttcttggcc gtgccgagaa
     8401 cctcgccggt gtcgagatta accacgccgg cgagcacctt cgttccgccg aaatcgatcc
     8461 ccagtgcgat gctcattcgc tcaccctctc gcctgcctgc cgtgaatatc gctacagtac
     8521 gccagacagt ggcacacatg cggcagaagg gcgacatgac cggcagaacc atcccgttca
     8581 ccaagatgag cggcagcggc aacgatttcg tggtgatcga caatcggtcc ggcgtgctgg
     8641 accggttgga tattccggcc ttcacgcgcg cggttagccg ccaccgaatt tcggtcggcg
     8701 ccgatggcgt ggtgatgctc tcggagccac ctgagggcga tcgtacgctt tcctacgcct
     8761 ggcgctacgt caatgccgac ggatcggacg gcgagatgtg cggcaacggg gcgatgtgcg
     8821 cagcccggtt cgcggtccgg accggcatcg ctgagccgca tcactggttt ctgacaccga
     8881 gcggaatcgt cgaggcgtgg gcagaggcag cgagcccgtc ggcagcgatc gcgatcgccg
     8941 atccagagcg gatcgaggag ccggtcgagc tgtcggtgct cggtcggacg ctctgctgcg
     9001 ttcgagtcgt ggtcggcgtg ccgcacgtcg tgatccgggt cgacgatgcc gatgcatttg
     9061 ccgacgccgg tacattccac gccataggca gcgcgctccg cctgcacgac gtcttcgcgc
     9121 cggccggtac gaacgcaaac gtggtctccc gaatcgacga cgagacctgg cgaatgcgaa
     9181 cctacgagcg gggcgtcgag gccgagaccc ttgcctgcgg aacgggagcg gtggcgagtg
     9241 cgatcgtgct cggacatcag ggactcgcgc aatcgccaac ccggatcagg acctcgagcg
     9301 gccgcgatct gaccgtgagc tatgacctga acgacagtca ggccacgcac gtgcgcctcg
     9361 ccggtcacgc cgccatcatc tacgacggca ccctcaccga ggaagcattt cagggataga
     9421 gtcgatttcg aagagccagg caacgtcgcg cgggcttacc atgccccgcc cggatctgag
     9481 gatggctcat tcgctggggg aaccaacccc ggatgcggtc cggcttcatt ccccgtgcgt
     9541 gggaatgcat caagcggggc gtggtaaccc cgcgctacgt ccaataccag aattcctggt
     9601 ctgagatcag gactcttccc agaagacgcg acggaattcg ttggagtcgt aataaccgac
     9661 gatgttgacg atacgaccct gcgcggcccg catcacgacc acctgctccg agagcagatc
     9721 acgcccgagg cgggtcgaga actcccgcac cacgaactgg accgccgccc gatccccgct
     9781 cgcgataacg tcgctgacct cccagtgcgc cgaggggtat gaggagacaa ccttgccgaa
     9841 gtaggcgacg atgtcgttgg ccggccggat cggctggctg tgcggcaacg cccgg
//
LOCUS       DATAOZ010000262         6619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8266755, whole genome shotgun sequence.
ACCESSION   DATAOZ010000262 DATAOZ010000000
VERSION     DATAOZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8266755"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VHR64_06955"
     CDS             <1..572
                     /locus_tag="VHR64_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX3302605.1"
                     /translation="GTLVTLGSDGLTANHIPFLLNANDGPHGTLIGHVARNNALWRDH
                     AGTVEALVIFQGASAYISPNWYVTKQVTHEVVPTYNYAVVHAHGPLVIHDDPKWLRGV
                     VGKLTKAMEASQAVPWKMADAPSGFINQQLENIVGIEIPISRLTGKWKTSQNRPVDDR
                     VGAAAGLRVSGTPGDTEMAELIERAIKLS"
     gene            803..1009
                     /locus_tag="VHR64_06960"
     CDS             803..1009
                     /locus_tag="VHR64_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302606.1"
                     /translation="MELDPMVIPIGAEVRSQTGDLIGRAREVHMRYLLLEMSDGDEVE
                     IPVTSILHIEGKRVVVDESVMETE"
     gene            1038..2099
                     /locus_tag="VHR64_06965"
     CDS             1038..2099
                     /locus_tag="VHR64_06965"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX3302607.1"
                     /translation="MTHETETFALIRTLTELPGPTGHEDAVQDWIFDRWRQSAQEICR
                     SRVNNVLARIGGRGRRLALMAHADEICFMVKSITDEGFLHIWPYYQDLRGHPPRWVLP
                     LNQHALVMTSGEPVEGYFATASGHVVTNREPAAKWSWNDWFIDIGVSSRQEAEALGIG
                     PGCHAIWNPPTRRLRNGRITGKAMDDRAALAIATLAGERLAGRTDLAYEVWLVSTIQE
                     ENGLIGAQSIVDEIELDLAINLDVGLTGDVPGVDARDFPAKLGRGPIVVYQDGSAHYS
                     RKLSDRLLSIAQQEEIPVQRAVSQQYGSDAAALLRRGVDVSLLTYPTRYTHSPIETID
                     EADIERCVDLLVAFATTPE"
     gene            2198..3652
                     /locus_tag="VHR64_06970"
     CDS             2198..3652
                     /locus_tag="VHR64_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX3302608.1"
                     /translation="MKAWLSNLRLRFLLLVLLAVLPAVVLLLLTVSEQRDEAVKKGQA
                     NAQQLAGLATADQGRLIESTRQLLTLLALLPEVQEGGTACNELFASLLKQFPLYANLG
                     VIGADGMVTCSGIPISEPINLGDRTYFQHAVESKSFVVGEYQTGRITGKPALNCGYPV
                     IDANGNVTEVVYAAIDLNSIAQQFASEAQLPEGAILTVLDRTGHVLARVPEQPELIGA
                     SLVGTPVVDEILAQGSGRAEESLDGTTYVIAYQSLGSSDQVSAYLSVALPKGDITASA
                     EKQFGDNLTRLALAVMVIVIAAWVGTDLLVRQSPEIYKSVVRRYYDAFSTGGTDLLDD
                     VVADDFVDHDPMPGQPPGLVGIKLAVDSFRAAFPDGEMVVDSLIAEGDRVVARVTMSG
                     THRGEYAGMPGTGKAVRAEGMEIFRLADGKLAEGWSRFVLPLAMIERDSSSQIPEDGD
                     ERADIDLDGAVRKSTGPVRSLIRSAGRLIGLGGQ"
     gene            3655..4650
                     /locus_tag="VHR64_06975"
     CDS             3655..4650
                     /locus_tag="VHR64_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX3302609.1"
                     /translation="MVINLNGVYGGRGAAPVTPATVLRRQTQGAIKAQHPGVIKRIER
                     VLRWMHRGYVQANAGDLAAAVAYSALIALIPTMLLTLSVAGLFFRIDSIFKQTIYTSF
                     WGLPSSAAGDAIQTVLTARRTSSWLGVISLAWFAWSGTGFVGCLSRSMNRIYGVTGCG
                     YMCEKRRGFFVVLAFALLFMLALLASTIPTLFVRQDLPSYFQSWALAAGWNQVLGYLI
                     AFLATGAMFLMLYRVVPNAGQEIADVWPGTLNGAFLFVIIAQVFPIYIRLLGGFNRYG
                     AAFGLLSLLVVWFWVLAHLLLFGAYINSTYQRHRRSEERRARRRAQRRQRLSHQS"
     gene            complement(4617..5345)
                     /locus_tag="VHR64_06980"
     CDS             complement(4617..5345)
                     /locus_tag="VHR64_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX3302610.1"
                     /translation="MRIGLISDIHGNLVALDTVLAELDQAGVEQIVCLGDLAVLGPQP
                     AGVVDRIRERGIPTVRGNVDDWLAPNQGPAAEPPESTQSIELSAWTLAQLDASQIDFL
                     RRLPLTRHVPLGDGRSMLCFHATADSVDDITHAGEPGLVGEWPDEPMMACGHTHIQAM
                     WRVGDQIWINPGSVGLPGIGPGTPGLPRNHNVSWAEFAIIETDHRGMSVDMRRTELDI
                     GAIWAAARATDMPHQDWWLNLWRL"
     gene            complement(5358..6116)
                     /locus_tag="VHR64_06985"
     CDS             complement(5358..6116)
                     /locus_tag="VHR64_06985"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3302611.1"
                     /translation="MSDKSRQRVVIVTGAARGIGRAIAERFHRDGAIVVGLDIEPVAA
                     TNDGRLTGVVCDVSDSAAVDKAVADVVKEHGRLDVMVCNAGIGGGAPVAELSDELFRR
                     IIDVNLFGVFACCRAAARAMIPKRSGVIVTIGSVFGQDPPAGSAAYGAAKAGVAALTR
                     SLARELGPHGIRVNCVSPGHIQTEMYAAALERRAAANGITLDEATMRERNPIPLGQFG
                     TPDDVAKVVAFLASDDGAYVTGQRINVDGGLQPL"
     gene            complement(6113..>6619)
                     /locus_tag="VHR64_06990"
     CDS             complement(6113..>6619)
                     /locus_tag="VHR64_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302612.1"
                     /translation="IPRLDRGLWDTVVNVMAEKSSTATYDGGTGNIDVTLFMCPDSAT
                     PKTADDAARTCTEPAAGSEVQAVGKDGTMYASATTDNQGKAQIALSGQTVPEFWIVFE
                     GHSTPGSPISCVANRMDPQTGKPLPPLYQATTDDPNTGWTIPGLGDDMNGISCDWYLV
                     PGGWKQSS"
BASE COUNT         1170 a         2057 c         2147 g         1245 t
ORIGIN      
        1 tcggcacgct cgtgacgctc ggatcggacg ggctgaccgc caaccacatt ccgtttctgc
       61 tcaatgcaaa cgacgggcca catggcacgc ttatcggtca cgtggcccgg aacaacgcac
      121 tctggcgtga ccatgccggg acggtcgagg cgctggtgat cttccaggga gcgtcggcgt
      181 acatatcgcc gaactggtac gtgaccaagc aggtcacgca tgaggtcgtg ccgacctaca
      241 actatgcggt tgtccacgcg catggaccgc tcgtcatcca cgacgatccg aaatggctgc
      301 gcggcgtggt tggcaagctg accaaggcga tggaggcttc gcaggccgtg ccgtggaaga
      361 tggccgacgc gccgtccggc ttcatcaatc agcaactcga gaatatcgtc ggcatcgaga
      421 ttccgatcag ccggctcacc ggcaaatgga agaccagcca gaatcgccct gtcgacgacc
      481 gcgtcggcgc ggccgcgggt ctgcgcgtgt ccggaactcc aggggacacc gaaatggcgg
      541 agctgattga gcgggccatc aaactgtcgt agccgatggc aagaatcccg ctgggggagc
      601 ggtttcgggt tcgtcgattt cccttttgcg gataagatgc gttgtgcgaa gtacacaatt
      661 tgagcctgct gcgcgacata atggtgacac ttatgtcccg ccggagggaa gtgattcact
      721 gcaagcatcg cgcaccctct tgcctgactt ggtacgctga ccagtgagat atgggttggc
      781 tgttgacgag gaggccaacg gcatggagtt ggatccaatg gtcataccga taggcgcaga
      841 agtccgatcc caaaccggag atctcatcgg ccgggcccgc gaggtccata tgcgctatct
      901 gttgcttgaa atgtccgacg gtgacgaggt cgagatcccg gtcacgtcaa tcctgcatat
      961 cgaaggcaag cgcgtggttg tcgacgagtc cgtcatggaa accgaatagc tcaacgaaag
     1021 gcagttccgg gactcgaatg acccacgaaa ccgagacgtt tgcgctgatc cggaccctca
     1081 ccgaactccc gggtccgacc ggccatgaag acgctgtcca ggattggatc ttcgaccgct
     1141 ggcgacaatc tgcccaggag atttgccgtt cccgcgtcaa taacgttctt gcccgcatcg
     1201 gtggccgcgg ccggcgcctc gcgctgatgg cgcacgccga tgaaatctgc ttcatggtca
     1261 agagcatcac cgacgagggt tttctgcata tctggcccta ttatcaggat ctccgcggtc
     1321 atcccccacg ctgggttttg ccgctcaatc agcatgcact tgttatgaca agcggcgagc
     1381 ccgtcgaggg ctacttcgcg accgccagtg gtcatgtggt cacgaaccgc gagccggcgg
     1441 ccaagtggag ctggaacgac tggtttatcg acatcggcgt ttccagccgg caggaagcag
     1501 aagctctcgg aatcggtccc ggctgccatg cgatctggaa tccgccgacg cgtcgcctgc
     1561 gcaacgggcg gatcaccggc aaggccatgg atgaccgggc tgcgctggcg atagcgacgc
     1621 tggccggcga gcgcctggcc ggccgaaccg acctggccta cgaggtctgg ctcgtctcga
     1681 cgatccagga agagaacggg ctgatcggcg cccagagcat cgtcgacgag atcgagctgg
     1741 atctggcgat caacctcgac gtcggcctca ccggcgatgt gcccggcgtc gacgcgcggg
     1801 attttccggc aaagttgggc aggggtccga tcgtcgtcta ccaggacggc tctgcccatt
     1861 attcgcgcaa gctctccgac cggctcttgt caatcgcgca gcaggaggag atcccggtcc
     1921 agcgcgcggt gtcccagcag tacgggagcg atgccgccgc cttgctcagg cgtggcgtcg
     1981 acgtatcgct tctgacgtat ccgacgcggt acacccattc gccaatcgag acgatcgacg
     2041 aagcggacat cgagcgctgc gttgatttgc tggtggcgtt cgcgacgaca cccgaatagg
     2101 cagctccccg ctatagactc agagaagatt cgcgcagtgg cgatccgacg cccttgtgcg
     2161 caggtcgacg caggcgccag gagggccgat cagtaaggtg aaggcgtggc tctcgaacct
     2221 gcgattgcgt ttcctgctgc tggtcctgct tgcggttctg ccggctgtcg tgttgctatt
     2281 gctcaccgtc tccgagcaac gcgatgaggc ggtgaaaaag ggccaagcga atgcccagca
     2341 actggcggga ctagccacgg ccgaccaggg ccgcctgatc gagagcactc ggcagctcct
     2401 gaccctgctg gccctgctcc ccgaggtcca ggagggcggt acggcctgca acgagctctt
     2461 cgccagcctg ctgaagcagt ttcccctcta cgcgaatctc ggcgtgatcg gcgcggacgg
     2521 aatggtcacg tgcagtggca tcccgatctc tgaacccatc aacctgggcg accggaccta
     2581 tttccagcac gcagtcgagt cgaagtcgtt cgtcgtgggt gaataccaga cgggtcgcat
     2641 taccgggaaa ccggccctga actgcggcta tccggtgatt gatgcgaacg gaaatgtgac
     2701 cgaggtggtc tacgcggcga tcgacctcaa ctcgattgcg cagcagttcg cgtcagaagc
     2761 gcaactgccg gagggcgcaa tcttaaccgt gctcgaccgc accgggcatg tgcttgccag
     2821 ggttccggag caacccgagc tgatcggcgc gtcgctggtc gggacgccgg tggtcgacga
     2881 gatcctggcc caggggagcg gccgcgccga ggagtcactg gacggaacga cgtatgtaat
     2941 tgcctaccag tcgctgggta gctccgatca ggtcagcgcc tacctgagcg tggccctgcc
     3001 gaaaggcgac atcacggcgt cggcggagaa gcagttcggc gacaacctga cgcggctcgc
     3061 cctggccgtc atggtgatcg taatcgcggc ttgggtcggg accgacctgt tggtccggca
     3121 gagccccgag atctacaagt cggttgtgcg ccggtactac gatgctttct cgactggcgg
     3181 aaccgatctc ctggacgacg tcgtcgcgga cgacttcgta gaccacgacc cgatgcctgg
     3241 tcagccgccg gggttggtcg gcatcaagtt ggcggtggat tccttccggg ccgccttccc
     3301 ggacggcgag atggtggtcg attcgttgat cgctgaagga gaccgggtgg tagcccgggt
     3361 cacgatgagc ggcacgcatc gcggcgaata tgccggtatg ccaggtacgg gcaaggccgt
     3421 ccgggcggaa ggaatggaga tcttccgctt ggccgacggc aagctggccg aaggatggag
     3481 ccggttcgtc ctgccgctcg ccatgatcga gcgggacagc tcgagtcaga ttccggagga
     3541 cggcgacgag cgcgcggaca ttgatttgga tggggcagtg cgcaagtcga ccggtcccgt
     3601 ccgatccctg attcgctcgg cgggtcggtt gatcggcctc ggaggtcagt agcgatggtg
     3661 atcaacctga atggggtcta cggcgggcgc ggagctgcgc cggtcactcc ggcgactgtc
     3721 ctgcgccggc agacccaggg agcgatcaag gctcagcacc cgggggtgat caagcgaatc
     3781 gagcgagtac tgcgctggat gcatcgcggg tacgtccagg cgaatgctgg ggatttggcg
     3841 gccgcggttg cctacagcgc cctgatcgcg ctgatcccga ccatgctcct gacgctctcc
     3901 gtggcgggtc tcttcttccg gatcgacagc attttcaagc agacgatcta caccagcttc
     3961 tggggcttgc cgagcagcgc cgcgggcgac gcgatccaga ccgtgttgac ggcgcggcgt
     4021 acaagtagct ggctcggcgt gatcagcctg gcctggtttg cctggagtgg gaccggcttc
     4081 gtgggctgcc tgtcgcggag catgaatcgc atctacggcg tgacaggctg cggatacatg
     4141 tgtgagaagc ggcgcggatt cttcgtcgtc ctggcgtttg cgctgctctt catgctcgcc
     4201 ctgctggcgt cgaccattcc gacacttttc gttcggcaag acctaccgag ttacttccag
     4261 tcctgggcgt tggccgcggg atggaaccag gtgctcggct atctgatcgc gttcctcgcg
     4321 accggcgcga tgttcctgat gctttaccgg gtcgtgccga atgccggaca ggagattgcc
     4381 gatgtctggc ccgggacgct gaacggcgca ttccttttcg tgatcatcgc gcaggtcttc
     4441 ccgatctaca ttcgcctact gggcgggttc aaccggtatg gcgccgcgtt cggattgctt
     4501 tcgctgctcg tcgtctggtt ctgggtattg gcgcacttgc tgctgtttgg ggcgtacatc
     4561 aactcgacgt accagcggca ccgacgatca gaggaacgcc gggccaggcg acgcgctcaa
     4621 aggcgccaga gattgagcca ccaatcttga tgcggcatgt ccgtggctcg tgcagcggcc
     4681 cagatagcgc ctatatcgag ctccgttcgc cgcatgtcca cagacattcc acggtggtca
     4741 gtttcgatga tcgcgaattc ggcccacgac acgttgtggt tccgtggtag gcccggcgtg
     4801 ccgggaccga tcccgggcaa gccgacactg cccgggttga tccaaatctg atcgccgacg
     4861 cgccacattg cctgaatgtg cgtgtgaccg cacgccatca tcggctcgtc gggccactcc
     4921 cctactagtc ctggctcgcc ggcatgcgtt atgtcatcaa ccgaatccgc tgtggcatgg
     4981 aaacagagca tcgaacggcc atcgccgagc ggcacgtgac gcgtcaaggg cagccggcgc
     5041 agaaagtcga tctggctcgc atcgagctgg gccagtgtcc aggcagacaa ctcgatcgac
     5101 tgggtcgatt ccggtggctc ggcagctggt ccctgattgg gcgcgagcca gtcgtcgacg
     5161 ttgccgcgga ccgtcgggat cccccgctcc ctgatccgat caacgacgcc cgccggctga
     5221 ggaccgagca cggccaaatc accgaggcag acgatctgct cgacaccggc ctggtcaagc
     5281 tcggccagca ccgtgtcgag cgcgaccagg ttcccgtgaa tatccgaaat cagtccgatg
     5341 cgcatggtcc tcggccgcta gagtggctgg agcccgccgt cgacgtttat ccgctgaccg
     5401 gtgacgtacg ccccatcgtc ggaggcgagg aatgcgacca ctttcgcgac atcatctggc
     5461 gtaccgaact gaccgagcgg aatcggattg cgctcgcgca tggtcgcctc gtcgagcgtg
     5521 atcccgttcg ccgcggcccg acgttcgaga gccgccgcgt acatctcggt ctgaatgtgg
     5581 cctgggctga cgcaattgac cctgatgccg tgcggcccaa gctcccgcgc tagcgacctg
     5641 gtgagggccg cgactcctgc cttggccgcg ccatatgcag cggagccagc cggcgggtcc
     5701 tgtccgaaga ccgagccgat cgtgacgatg acgccggatc ttttcgggat cattgccctg
     5761 gcggctgccc ggcagcaggc gaagaccccg aacaagttga cgtcgatgat gcggcggaag
     5821 agctcgtcgc tcagctccgc gaccggtgct cccccgccga tcccggcgtt gcagaccatg
     5881 acatcgagcc gaccatgctc cttgacgacg tccgcgaccg ccttgtcgac cgcagccgaa
     5941 tcgctgacat cacagacgac gccggtcaac ctgccgtcat tggtcgccgc caccggctcg
     6001 atgtcgaggc cgacgacgat cgcgccatcg cggtggaatc gctccgcgat ggcccggccg
     6061 attccgcgcg ccgcaccggt cacgatgacg acgcgctgtc gggatttatc gctcatgaag
     6121 actgcttcca gccgccgggg accagatacc agtcgcagct gatcccgttc atgtcgtcac
     6181 ccagtccggg gatcgtccaa cccgtgttgg gatcatctgt cgtcgcctgg tagagcggag
     6241 ggagcggctt gccggtttgc gggtccatgc ggttggcgac gcaggagatc gggctgcccg
     6301 gcgtcgaatg tccctcgaag acaatccaga attcgggtac ggtctgcccg gagagggcga
     6361 tctgtgcctt gccctggttg tcagtcgttg ccgaggcgta catcgtgccg tcctttccga
     6421 cggcctgaac ctcgctgccg gctgccggct cggtgcacgt cctggcagcg tcgtcggccg
     6481 tcttcggcgt ggcactatcc gggcacatga agagcgtgac atcgatgttg ccagtcccgc
     6541 cgtcgtaggt cgcggtcgag ctcttctccg ccatgacgtt gacaaccgtg tcccagagac
     6601 cacgatcgag gcgcgggat
//
LOCUS       DATAOZ010000263         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_372364, whole genome shotgun sequence.
ACCESSION   DATAOZ010000263 DATAOZ010000000
VERSION     DATAOZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372364"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /gene="dxr"
                     /locus_tag="VHR64_06995"
     CDS             <1..1182
                     /gene="dxr"
                     /locus_tag="VHR64_06995"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX3302613.1"
                     /translation="ALEVVAEHPDEFRVVGLAAGGGQIELLARQALDTGARTVAVSRA
                     TTVQDLQLALYGEASRRGWAEGKVQLPRILAGPEAAAELAATACDVVLNGITGSTGLA
                     ATLAALEAGRILALANKESLVVGGPLVNRAAQPGQIVAVDSEHSALAQCLRGGAASEV
                     DRLILTASGGPFRGMTREQMGDVTPDQALAHPTWNMGRVVTTNSATLVNKGLELLEAH
                     LLYEVELDRIDVVVHPQSIVHSMVQFVDGSTLAQCSPPDMKLPIALGLSWPDRLHGVA
                     QACDWSTATSWTFEPLDQTAFPAVELARQAGRLGRTAPAVYNAANEVCVDAFHDGRIR
                     FTEILDIVATVLRQHINSDADVGSTMVASDQLTLEMVLAADAWARARATDLTHATEPT
                     R"
     gene            1183..>2124
                     /locus_tag="VHR64_07000"
     CDS             1183..>2124
                     /locus_tag="VHR64_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3302614.1"
                     /translation="MELVIYIAGALVFFGLVMASIALHEIGHLVPGKLFNVKTTEYFI
                     GFGRKLWSRRRGETEYGVKAVPVGGYVRLVGMFPPSKDHPGQVRTYSTGPFRAIADNA
                     RAAEWSAIRPEDEGRLFYEKPFWQKLIIMASGPTMNILLAFLILLGVSLSYGVYRSQL
                     TISTVQECIVVANATDKSCAGKPPTPAAQSGIQPGDKIVAFNGTPVSSWDDVSRLIRS
                     NLDHPALLTVERAGQRIELKPVNTVITGVPDRYDPSRRVAAGFFGVEPEIIRERGGPV
                     VVIGDMWQMTKQTAVALAYFPVKVYNTAYNLATGKPRD"
BASE COUNT          389 a          638 c          699 g          398 t
ORIGIN      
        1 gctctggaag ttgtcgccga acatccagac gagtttcggg tggtaggtct tgctgccggg
       61 ggcggccaga tcgaactact cgcccggcag gcactagaca ccggtgcacg gacggttgcc
      121 gtcagccggg cgacaaccgt gcaagacctg caactggctc tttacggcga agccagccga
      181 cgcggctggg cagagggaaa agtgcaactg ccgcgcatcc tcgcgggccc ggaagcggcc
      241 gcagagctgg cggcaacggc ctgcgatgtc gttttgaacg ggatcacggg ctcgaccggg
      301 cttgcggcaa cgctggccgc gctcgaagcc gggcggatcc tcgcgctggc gaacaaggag
      361 tcgcttgttg tcggcggccc gctggtcaat cgggccgcac agcccggtca gatcgttgct
      421 gtcgactccg aacactccgc attggcgcaa tgtctccgcg gaggtgccgc gagcgaggtg
      481 gaccggttga tcctgaccgc aagcggtggg ccgtttcgcg gaatgactcg cgaacagatg
      541 ggcgatgtca cgccagatca ggctcttgcg cacccgacgt ggaacatggg ccgggtagtg
      601 acaacgaact cagcgactct ggtgaacaaa gggctggagt tgctagaggc acatctgctg
      661 tacgaggtgg agctggaccg catcgatgtc gtcgtccatc cgcagtcgat cgtgcactcg
      721 atggtgcagt tcgttgatgg atcgaccctc gcgcaatgct caccgccgga catgaagctt
      781 ccgatcgcgc tcggtttgag ctggccggac cggctccacg gagtagctca ggcgtgtgac
      841 tggagcacgg caacgagctg gaccttcgag ccgctggacc agacggcatt cccggcggtc
      901 gagctggctc ggcaggccgg gcgactgggt cgtaccgctc cagccgtcta caacgccgcc
      961 aatgaagtct gcgtggacgc ttttcacgac ggtcgcattc ggttcaccga aatcttggac
     1021 attgtggcca ccgtgttgcg ccagcacatc aattcggatg ccgacgtagg ctcgacgatg
     1081 gtcgcaagtg atcagctcac cctcgagatg gtgctggcgg ctgatgcctg ggcgcgcgct
     1141 cgggcgaccg acctcaccca cgcaacggag cccacgcgct agatggaact tgtgatctac
     1201 atcgccggtg ccctcgtctt ctttggcttg gtgatggcct cgattgcctt gcacgaaata
     1261 ggacatctcg ttccgggcaa gctgttcaac gtcaagacga ccgagtactt catcggcttc
     1321 ggccgcaaac tgtggtctcg ccgtcgaggc gaaactgagt acggcgtcaa ggccgttccg
     1381 gtgggcggct atgtccggct ggttggcatg tttccgccgt ccaaggatca tcctggtcag
     1441 gttcggacct acagcacagg gccatttcgg gcgattgccg acaacgccag ggctgcggag
     1501 tggtcagcaa tccgcccgga ggacgaggga cggctcttct acgagaagcc gttctggcag
     1561 aagttgatca ttatggccag cggcccgacg atgaacatcc tgcttgcctt cttgatcttg
     1621 cttggggtgt cgctgagcta cggcgtctat cgctctcagc tgacgatcag cacggtccag
     1681 gagtgcattg tcgtcgcgaa tgcgaccgac aagagttgcg ctgggaaacc gccgacgccg
     1741 gccgcgcagt ccggaataca gccgggggac aagatcgtcg cgttcaacgg aaccccggtg
     1801 tcctcctggg acgatgtgtc tcggctgatc cgatcgaatc tcgatcatcc ggccctgctg
     1861 accgtcgagc gggccggtca gcgcatcgag ctgaagccgg tcaacacggt cattaccggc
     1921 gtaccggaca ggtatgaccc gtcgaggcgt gtcgccgctg ggttcttcgg cgttgaacct
     1981 gagataatcc gggaacgcgg tgggccggtt gttgtcatcg gcgacatgtg gcagatgacc
     2041 aagcagaccg ctgtggcgct ggcctacttc ccggtaaagg tctacaacac cgcgtacaac
     2101 ctggcgaccg gcaagccgag agac
//
LOCUS       DATAOZ010000264         3248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8792524, whole genome shotgun sequence.
ACCESSION   DATAOZ010000264 DATAOZ010000000
VERSION     DATAOZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8792524"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            566..1468
                     /locus_tag="VHR64_07005"
     CDS             566..1468
                     /locus_tag="VHR64_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3302615.1"
                     /translation="MAYRFLLEVPESLVDEANIVVGSVGDAQVVVVRNSHGLGFDDPY
                     QDLTIAAHSLLVINAIYDWLESLGQSRPAMSLVLHSGQRLDLADYDRGRMIAAIRYDQ
                     PWVERTLPKIGEHYREILPPGDTRTEGIAPQPMTHLAAVVEETHPIIAATKQLTYRSP
                     VHVLVKVPDLDRAERFYVDFVGVHLLGRERVDAAGELQLVERDYHGPRALTLGTEADF
                     SYLANEPLRITLQRVGRGARLPQDNPEVTAVRVDHDSFVRLRGDALMRGSVILQDSPE
                     AFAVRDIYGLIWQFVDTADVPNPA"
     gene            1474..1887
                     /locus_tag="VHR64_07010"
     CDS             1474..1887
                     /locus_tag="VHR64_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302616.1"
                     /translation="MLDAFRELIDELLSAPAALRDAGLDSTTDEQALAALALITERDA
                     ALLDRIQKTIRTNSPVLKAPPAVPSEESLVGSPADLMARFDTGRGELVSLLMNLTLKD
                     WERTAIDWNGEEISVADDVEQHVEYDEQVQDELAG"
     gene            1916..2212
                     /locus_tag="VHR64_07015"
     CDS             1916..2212
                     /locus_tag="VHR64_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HEX3302617.1"
                     /translation="MHGAFKTPGGKLVQVDFEIADERLRNVEVSGDFFLYPEEALTKI
                     TAAVEGSPADMTMTDRAATIAAAIDHGVEWLGSSPEALATAIDRALLASMDTYE"
     gene            2205..2984
                     /locus_tag="VHR64_07020"
     CDS             2205..2984
                     /locus_tag="VHR64_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEX3302618.1"
                     /translation="MSDLSRWRYDPWVLLPIEPATPAMNLALDEELTLRVGADERPPT
                     LRFWSWTASCIVLGRFQSVRNEVQEENARARGVEIVRRISGGGAMFIEPEGAITYSLY
                     APAEMAAGMSFAESYAFFDSWVISALRSLGIDAWYAPLNDITSAGGKIGGAAQARRGG
                     AILHHTTMAYQMNIPAMMEVLRIGQEKLSDKGIRSADKRVGPLRQQTDLPRQQIIDHL
                     IAEFGNLTDLRESTLSDDERAGAETRVRERFGTDAWRYILP"
BASE COUNT          574 a         1132 c         1006 g          536 t
ORIGIN      
        1 acggaaccat tgttcgcgga cgcctggtcc gcgaaaacag gagtgctccc agccagagga
       61 gccaacaggg ttatggcgcc cgccatgaac ccgcgccgtg taagcgtgcg cattggtgtg
      121 tttccctcga gtcgttcctg cttctggcgg cgagggccaa gccacttcgc cgcgcgcatc
      181 ccgcagccac gggatgggat atgtcgtgct tctctccgaa ctcctgcttc actccgccgg
      241 cccgcagcca gtagaccggc tcacctgctc ttttgctccg cgccctctgc tccgtccatc
      301 cccgacagac acagctgcgc cccggcccat cgcccatcct cgcgataacc tgtgcaaaac
      361 tccatcttca ccgatgatgc ggcacaaact accatgaatt accaagattc gaccacccgg
      421 gatcgggcac gatgagccag gtacctgggt cctgaggtcc cgtaccgcgc ggtcgcgatg
      481 gtcaccgacc ggctgataca atcggcacga aatggcggga gacgcattcc gcccaccgta
      541 cgcgctgcac tgggagagat cgctcgtggc ctaccggttc ctgctggaag tgcctgaatc
      601 gcttgtcgat gaggccaaca tcgtggtcgg ctccgtcggc gatgcgcagg ttgtcgtagt
      661 ccggaactcg catgggctcg ggttcgacga tccctaccag gacctgacga tcgccgctca
      721 ttccctgctc gtgatcaacg cgatctacga ctggctcgaa agtctcgggc agtcccgccc
      781 ggccatgagc ctggtgctcc acagcggcca gcgactcgat ctcgccgatt acgaccgcgg
      841 ccgcatgatc gccgccatcc ggtacgacca gccctgggtg gaacgcaccc tgcccaagat
      901 cggcgagcac tatcgcgaga tcctgccgcc cggcgatacc cgcaccgagg gcatcgcgcc
      961 gcagccgatg actcacctgg ccgcggtcgt cgaagagacg cacccgatca tcgccgcgac
     1021 caagcaactc acctaccgct caccggtcca cgtgctggtc aaggtgccgg acctcgatcg
     1081 ggccgagcgc ttttacgtcg acttcgtcgg tgttcacctg ctcggtcgag agcgggtgga
     1141 tgccgccggc gagcttcaac tcgtcgagcg cgactaccac ggcccgcgcg cgctgaccct
     1201 cggcacggaa gccgatttca gctacctcgc caacgagccg cttcggatca cgctccagcg
     1261 ggtcggtcgc ggcgcccgcc ttccgcagga caacccggag gtgacggcgg tccgggtcga
     1321 tcacgacagc ttcgtccgat tgcggggcga tgccctgatg cgtggctccg tgatcctgca
     1381 ggactcaccg gaagcgttcg ccgtgcgcga tatttacggc ctgatctggc aattcgtgga
     1441 tacggccgat gtgccgaacc cagcctgacc gccgtgcttg acgccttccg cgaactgatc
     1501 gacgagctgc tctctgcccc ggccgcgctc cgcgatgccg gtctcgactc gaccaccgac
     1561 gaacaggccc tcgccgccct cgccctgatc acagagcgag acgccgcact tctcgaccgc
     1621 attcagaaga cgatccggac caattcgccc gtcctcaagg cgccgccagc ggtaccctcg
     1681 gaggaatcgc tggtcggctc acccgccgat ctgatggccc ggttcgacac cggccgcggc
     1741 gagctggtct ccctgctcat gaacctcaca ctcaaggact gggagcggac cgcgatcgac
     1801 tggaacggtg aggagatcag cgtcgccgat gatgtcgagc agcatgtcga atacgacgag
     1861 caggttcagg acgagctggc aggctgatta ccgcaatccc gtaggacgat ttccgatgca
     1921 cggcgccttc aagacacccg gtggcaagct ggtccaggtt gatttcgaga tcgcggacga
     1981 gcgtctgcgc aatgtcgagg tcagcggcga cttcttcctc tacccggaag aggcgctgac
     2041 caagatcacg gccgcggtcg agggctcgcc ggccgatatg acaatgaccg accgggcggc
     2101 gaccattgcg gccgcgatcg accacggcgt cgaatggctc ggctcctcgc cggaagcgct
     2161 ggccacggcg atcgatcggg cactgctcgc ctccatggat acctatgagt gacctcagcc
     2221 gctggcggta tgatccgtgg gttctgctgc cgatcgagcc ggccacgcca gccatgaacc
     2281 tcgccctcga tgaggagctg accctccgcg tcggggccga cgagcggccg ccgactctcc
     2341 gcttctggag ctggaccgcg agctgtatcg tgctcggccg cttccagtcg gtccgcaacg
     2401 aggtccagga ggagaacgcg cgggcgcggg gtgtcgagat cgtgcggcgg atcagcggcg
     2461 gcggcgcgat gttcatcgag ccggaaggag cgatcaccta ctcgctctac gcgccggctg
     2521 agatggccgc ggggatgagc ttcgccgagt cctatgcgtt cttcgactcg tgggtgatct
     2581 cggcgctgcg ctcactgggc atcgatgcct ggtacgcgcc gctgaacgac atcacctcgg
     2641 ccggcggcaa gatcggcggc gcggcccagg cccgtcgcgg cggcgcgatc ctccaccaca
     2701 cgaccatggc ctaccagatg aatatcccgg ccatgatgga agtacttcgg atcggccagg
     2761 agaagctgag cgacaagggc atccgctcgg ccgacaagcg cgtcggtccc ctccgccagc
     2821 agaccgatct accgcgccaa cagatcatcg accacctgat cgccgagttc ggcaacctga
     2881 ccgacctgcg cgagagcacg ctctccgacg acgagcgcgc cggcgccgag acgagggtcc
     2941 gcgaacgctt cggcaccgac gcctggcgct acatcctgcc gtaatacggc gaacggggat
     3001 ggcgcctcga accgatctcc ctgcgtcgcc cgcggctaaa ggcgcgggct gaggaacgat
     3061 aagcccactg aaggggctgg aaaagcagtc ggcttcagcc gccttatcca ggctcagccc
     3121 cggccttcag cccggggcgg taacgggtga acgctcccat tgatcacacc gtcatttcga
     3181 ccgcgccgga ctgtaaccag agcgcgaggc cgatcgtttg ggactccgta accccggcga
     3241 cagccggc
//
LOCUS       DATAOZ010000265         3187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8267944, whole genome shotgun sequence.
ACCESSION   DATAOZ010000265 DATAOZ010000000
VERSION     DATAOZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8267944"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VHR64_07025"
     CDS             <1..852
                     /locus_tag="VHR64_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302619.1"
                     /translation="DAALPGDGPVRERNIAYRQQFVVDQPTAARLIDLILTETRLRTQ
                     QIIDLPPGDAVEIAFVSNQPWSGYNWYLGNARSRVDINTDLPIHAHALPGLVAHEAYP
                     GHHTEHALKDMRLYHGRGYAEQAIQLINTPECVISEGIAVLAEDIAFGDGQAEWMANV
                     LMPEAGLRFDPDVHRRVQRAGRQLRAVPSNAALLYHRDGRSEREVIQYLEHYGMMTEA
                     EAAKRFSFISDPLWRPYIFTYSVGRQLLSAWLDRAEGEDRIERFRRLLTEQITPSQVA
                     GQITARA"
     gene            857..1531
                     /locus_tag="VHR64_07030"
     CDS             857..1531
                     /locus_tag="VHR64_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302620.1"
                     /translation="MHRYDQPWPKVASPRDLLRRFNRWLSLTLIQQAIWPAIVLVAGA
                     VMHRPGESSIGDVLGMMAGPIVASLSAYMQIRDRLAEFPAPPVRSFVAEQARLLLLGL
                     PVMVLIGRLITGDFDATVKIAAVGLANVAAYHLIHFGVARSLFPSPYVVTVLFGLSWA
                     IHQVADALARDIGGSFIYHAFAGFTVGVLVAVASQILHRWPGGRLTAPSAQWLVLTLI
                     FGFSAS"
     gene            1843..>3187
                     /locus_tag="VHR64_07035"
     CDS             1843..>3187
                     /locus_tag="VHR64_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3302621.1"
                     /translation="MKNTSAGRRAAGAGLIAAMVMVAVQLLWRVMDSKDNVVQAFPEF
                     IAAAIARLTPLSFFGSVTENYGSLAKKSLLTVCIIGVIAIGPAAGRWAFTLTRERSPS
                     FWRRLGAGLVVAAGLLAVVLLVVMPIANLGIAARDSSYTSDILTQLFVTFALWAVVFA
                     VLSAEPQVREAAAGEQVTRRTALQSIGWSAVSLTALIGIIAALADMFKGTKLSDAEVA
                     AQEQAVDQIVQTERPAAAAATPGAATSASTPGAAFAQFDQLQADGHLTPVLTATKDFY
                     HVSKNFTDPTVSSAGWSLDIGGLVTTPKSYSYDELVGRSTVKNITTLCCISNELNGDL
                     ISTAEWTGVRLVDLLTEVGVAQNTVDLKLTAADDYTESIPLAIAQDPAVMIVTGMNGE
                     PLPDDHGFPARLIVPPIYGMKNVKWLKKIEAVDEDYKGYWQERGWSDTARYQIWGR"
BASE COUNT          532 a         1017 c         1046 g          592 t
ORIGIN      
        1 gacgctgccc tgcccggcga tggtccggtc cgggagcgga atatcgccta ccggcaacag
       61 ttcgtcgtcg atcagccgac tgccgctcga ctaatcgacc tgatcctgac cgagacgcgc
      121 ttacggacgc agcagatcat cgatctgccg cccggcgacg ccgtcgagat cgcgtttgtc
      181 tcgaatcagc cctggagcgg ctataactgg tacctcggca atgcgagatc gcgggtcgac
      241 atcaacaccg atctgccgat tcacgctcac gccctgccgg gtctggtcgc gcacgaggcc
      301 tatcctgggc atcacaccga gcacgcgctc aaggacatgc ggctctacca tggtcgcggc
      361 tatgccgagc aggcgatcca gctgatcaac acgccggaat gcgtcatttc cgaggggatc
      421 gctgtcctgg ccgaggacat cgccttcggg gatggtcagg ccgagtggat ggcgaacgtg
      481 ctgatgccgg aggcggggct gcggttcgac ccggatgtcc atcgccgggt ccagcgggca
      541 ggtcggcagc tgcgggcggt cccctcgaat gcggccctgc tctaccaccg ggacggccga
      601 tctgagcgcg aggtgatcca gtacctcgaa cactacggca tgatgaccga ggctgaggcg
      661 gccaagcgct tctcattcat cagcgatccg ctctggcgcc cgtatatctt cacctacagc
      721 gtcggccggc aactgctctc ggcgtggctg gaccgggccg agggcgaaga tcggatcgag
      781 cgcttccgcc ggctcctgac cgagcaaatt accccgtcgc aggtcgcggg tcagatcacc
      841 gcgcgggcct gaggcggtgc accggtacga ccagccgtgg ccgaaggtgg ccagcccgcg
      901 cgatcttctg cgccggttca accgctggct gagcctgacc ctgatccagc aggcgatctg
      961 gccggcgatc gtgctggtcg ccggtgcggt catgcatcgg ccgggcgaat cgtcgattgg
     1021 cgatgtgctc gggatgatgg ccggaccgat cgtcgccagc ctgagcgcct acatgcagat
     1081 ccgcgaccgg ttggcggagt ttccggcccc accggtgcga tcgttcgtgg ccgagcaagc
     1141 ccgactcttg ctgctggggc tgccggtgat ggtgctgatc gggcggctga tcacaggcga
     1201 tttcgatgcg acggtcaaga tcgcggcggt gggactggct aatgtagctg cctatcacct
     1261 gatccacttc ggtgtggccc gcagcctctt cccgagccca tatgtcgtga ctgtgctgtt
     1321 cggcctgtcg tgggcaatcc accaggttgc cgatgccctc gcccgggaca tcggcggctc
     1381 gttcatctac cacgcctttg ccgggttcac ggtcggcgtg ctggtggcgg tcgcgtcgca
     1441 gatcctgcac cgctggccgg gcggtcgcct gaccgcaccc tcggcccaat ggctcgtgct
     1501 caccctaatc ttcggcttct ccgcgtcgta gccacactcg tcgagaatta tgtaacgtag
     1561 cgcggggtta ccacgccccg catcattgct ccctatctac ggtcgatgca tctagaccgg
     1621 gaccgggatt cgctttccca gcccggtggc cgtcaatgga ctcgggcggg gcgtgatccc
     1681 ctctccaact ctcccctcaa gggaagagag catccccgcg ctacgtttcg gggcgatcgg
     1741 ttggggttta tgaagttatg ggccgcgtgg tagccggcac tcatccaggc tggggccgga
     1801 tacgtaaaag tgtcgttaca cgctctcgga agaaggcacg ttatgaaaaa tacaagtgca
     1861 ggacgacgag ccgcgggcgc cggactgatt gccgcgatgg tgatggttgc tgtgcagctc
     1921 ctctggcgcg tgatggactc gaaggacaac gtcgtgcagg cgtttccgga gttcatcgcg
     1981 gccgcgatcg cccggttgac tcccctctca ttctttggct cggtgaccga aaactacggc
     2041 agcctcgcca agaaaagcct gctgacggtc tgcatcatcg gcgtgattgc gatcggccct
     2101 gccgccggac gctgggcatt cacgctcacc cgtgaacgat ctccctcctt ctggcgaagg
     2161 ctgggggccg gcctggtcgt cgcggctggt ctgctggcgg tagtgttgct ggtggtcatg
     2221 ccgatcgcga atctcggcat cgccgcgcgg gacagtagct acaccagtga catcctgact
     2281 cagctcttcg tcaccttcgc gctctgggcg gtggtcttcg cggtgctctc ggccgagcca
     2341 caggtgcgag aggcggctgc gggcgagcag gttactcggc gcacggccct gcaatcgatt
     2401 ggctggagcg cggttagcct gaccgcgctg attgggatca ttgccgcgct ggccgacatg
     2461 ttcaagggca cgaagctgtc cgatgccgag gttgcggcgc aggagcaggc ggtcgaccag
     2521 atcgtgcaga cggagcgccc ggccgcggca gccgcgactc ccggggcggc gacaagcgca
     2581 tcgacgccgg gagccgcctt tgcccagttc gatcagcttc aagccgatgg acacctgacg
     2641 ccggtgctga ccgcgaccaa ggacttctat cacgtctcca agaacttcac ggatccgacc
     2701 gtgagcagcg ctggctggtc gctcgatatc ggcgggttgg ttacgacgcc gaagtcatac
     2761 agctacgacg agctggtcgg ccgctcgacc gtcaagaaca tcactaccct ctgctgcatt
     2821 tcgaacgagc tcaacggcga cttgatcagc acggctgagt ggaccggcgt gcggctggtc
     2881 gatctcctga ccgaggtcgg tgtggcccag aacaccgtcg acctcaagtt gaccgccgcc
     2941 gacgactaca ccgagtcgat cccgctcgcg atcgcgcagg atccggcggt gatgatcgtg
     3001 actggcatga acggcgagcc gctgccggat gatcacggtt tcccggcccg gctgatcgtg
     3061 ccgccgattt acggcatgaa gaacgtcaaa tggctcaaga agatcgaggc ggtcgacgag
     3121 gactacaagg gctactggca ggagcggggc tggagcgaca ccgcgcgcta ccagatctgg
     3181 ggccgga
//
LOCUS       DATAOZ010000266         5744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8793008, whole genome shotgun sequence.
ACCESSION   DATAOZ010000266 DATAOZ010000000
VERSION     DATAOZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8793008"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VHR64_07040"
     CDS             complement(<1..587)
                     /locus_tag="VHR64_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302622.1"
                     /translation="MSTTSPSSPFTISVAIDLDGVLTEHPRPLAIAANAKFGMDLPES
                     AFIDSAGLNVPMAVRDWVYGENGPASELFPSPDAQEFIARIIELLGMDHVRILTARPE
                     SSVDMTRAWLRRHGFPEINILFSDNKAEVAAAEGCTFAVEDSVRHAVNYARSGITSFL
                     IMTESTPAYESHQRIWPVNSLMSIIYRLDELQRENA"
     gene            807..1808
                     /locus_tag="VHR64_07045"
     CDS             807..1808
                     /locus_tag="VHR64_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302623.1"
                     /translation="MAGNWPGFEGAFLHGSITWLPDDAILPETSDVDLIVVLSDDGLP
                     PNPGKFRYDGVLLDASILPADQARSAEQILGISHLAGSLRGASILADPTGHLSELHEI
                     VARDFAKREWVIRRCEHARSKVLNFLDQIEPSAPLHDNVMSWLFGTGCTTHVLLVAGL
                     RNPTVRSRYIAVHDLLAEHGRLDIHEELLELLGAANLSRKQVEQHFAAMTEAFDATKQ
                     VVRTPVFFASDMSDVARPIAIDGTRDMIDRGYHREAMFWIAATQTRCQTILTAEAPQE
                     LHDRFTPGYRAMLSDLGIVAYDNLSQRSEAVRSYLRSLWTTAETIMEAPPAPADPQT"
     gene            complement(1827..2249)
                     /locus_tag="VHR64_07050"
     CDS             complement(1827..2249)
                     /locus_tag="VHR64_07050"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3302624.1"
                     /translation="MKVSAQVQNAYGEHQAVISTNGNTRSISIPPKMTGLGSSASGGE
                     LLFLALATCYCNDIYREADKRGISVERVEVEVNGDFGGPGDGASNVIFRAKVTADAPE
                     AEIDDLMRYTDTVAEIQNTLRAMTPVTLTSIEAVSKTR"
     gene            complement(2295..3560)
                     /locus_tag="VHR64_07055"
     CDS             complement(2295..3560)
                     /locus_tag="VHR64_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX3302625.1"
                     /translation="MPPVRITNVRAICTAPEGIRLVVVKVETSEPGLYGLGCATFMQR
                     PLAVVEALETYLRPQLIGRDPNDIEDIYQTLYLSSYWRTGPIMNNALSGVDQALWDIK
                     GKRAGMPVYQLLGGKVRTAATVYTHASGRDVQEVEDHARMLMGLGFTHIRAQVEIAGY
                     STYGAQGGSEPADVGTPAQAHVGNRPAHLEPVQRPWHESSYLRAVPKLFEHLRMTLGD
                     EIELLHDIHERVSPIQAIGLAKELEQFRLFFLEDPLAPEDIGWFPRLREKTSIPIAMG
                     ELFVNQTEWEMLIKDRLIDYIRVHISAIGGLSIARKLAAFAEFFGVRTAWHGPGDVSP
                     VGHAANLHLDISSTNFGIQELHLFNDTAREVFPGTPEVRDGMLWCNDQPGLGVDIDEA
                     LAAKYPFPEHELHGAFPAVRRIDGSVIRW"
     gene            complement(3626..5011)
                     /locus_tag="VHR64_07060"
     CDS             complement(3626..5011)
                     /locus_tag="VHR64_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX3302626.1"
                     /translation="MSTAAVMSITSINPATEEVNATYPIDSPEEVDRKIQHARDAFEI
                     WRDVPIEERAEGVRRLGAALRANKDKLAGLITREMGKTLPEAEAEVEKSAGNCDFYAD
                     NGPKMLEQEIVETTAREAYVSFEPLGVILAVMPWNYPIWQLIRHAAPALVAGNTIVLK
                     HASNVIGSALAMQDIAREAGLPDGAITALVIPGDRVEDVIADPRIAAVTLTGSDAVGS
                     KVAEAAGRHIKKTVLELGGSDAFVVLEDADLEKAADYAARSRFQNAGQVCISAKRFIV
                     VDKVADKFVELLKQKAEAIQYGDPTDRTVKMGPMSRSDLRDTIERQLRESMDAGADLV
                     TGGERPDGKGYYYPPTVVDNVTPDMTLFREESFGPVAAITRVPDEDAAIAAANDSPYG
                     LGSAIWTQDIERAKKLARKIEAGGVFINGMTTSNWKVPFGGVKRSGYGRELSSYGMRE
                     FVNIQTVWVEQ"
     gene            complement(5181..>5744)
                     /locus_tag="VHR64_07065"
     CDS             complement(5181..>5744)
                     /locus_tag="VHR64_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3302627.1"
                     /translation="LAGKQMTIVGPGRIGRQIARVAHAFDMRVTAVGRTGGSERAEAL
                     GVDHYLPIGRLHEALSTADVVVVVTPHTGGTERLIGAAEFDAIKPGVWFINIGRGAVV
                     DEQEMIARIRDGRIGFAALDVFQQEPLPPNSQLWDLPNTLISPHCSANAPRENERITD
                     IFIRNLPLFLAGRYDEMSPVLDKTQLY"
BASE COUNT         1006 a         1869 c         1799 g         1070 t
ORIGIN      
        1 gcattctcgc gctggagctc atcgagccgg tagatgattg acatcaggct gttgacgggc
       61 caaatccgct ggtgtgattc gtaggccggc gtgctctccg tcatgataag gaacgacgtg
      121 atcccgctgc gggcgtagtt gaccgcgtgg cgaacgctgt cctcgaccgc gaaggtacag
      181 ccctcggcag cggcgacctc ggccttgtta tccgagaaga ggatattgat ctccgggaag
      241 ccgtgccgcc ggagccaggc gcgagtcatg tcgaccgacg actccgggcg ggcggtcagg
      301 atacggacgt ggtccatccc caggagctcg atgatccggg cgatgaactc ctgcgcgtcg
      361 ggtgacggga atagctccga agcgggaccg ttctcgccat agacccagtc gcgcacggcc
      421 atcggcacat tgaggccggc cgaatcgatg aaggcagatt ccgggagatc catgccgaac
      481 ttcgcgttgg ccgcgatcgc gagcgggcgg ggatgctcgg tcagcacgcc gtctaggtcg
      541 atcgcgacac tgatcgtaaa cggcgacgag ggggaggtcg tcgacatgga agttcaattc
      601 tccgggtgac acgtgcatgg cggcgccggc gcaaccaaaa accgcgccgc gttgcgctca
      661 agagagccta gcaccctgtt gtgccgatca caatctgggc cgatcggtga caatggggcc
      721 aacgggtaca gggatcagtg ggtcagaggg cagtgaggct caccgtcgaa caggcgaaaa
      781 atgcggcgac gcggtgggtg tccgaagtgg ctgggaattg gcccggcttc gagggcgcat
      841 tcctgcacgg ttcgatcacc tggctgccgg acgacgccat cctgccggaa acgtccgatg
      901 tcgatctgat cgtcgtcctg tcggacgatg ggttgccgcc gaatccgggc aagttcaggt
      961 acgacggcgt cctgctcgat gcttcgattc tccccgctga tcaggcccga tcagccgagc
     1021 aaatcctcgg gatctcgcac ttggccggga gcttgcgagg agcaagcatc ctcgccgatc
     1081 caaccggcca cctgtccgaa ctccacgaga tcgtcgcgcg ggacttcgcc aagcgggaat
     1141 gggtgatccg gcgctgcgag cacgcccgat ccaaggtcct gaacttcctt gatcagatcg
     1201 agccgagcgc accgcttcac gacaatgtca tgtcctggct cttcggcacc ggctgcacga
     1261 cgcatgtcct gctcgtcgcc ggtctccgta acccgacagt ccgcagccga tacatcgccg
     1321 tccacgacct gctggccgaa catggccggc tcgacattca tgaggagttg cttgaactgt
     1381 tgggcgcggc caacctctct cgcaaacagg tcgagcagca ctttgccgcc atgaccgaag
     1441 cgttcgatgc caccaaacaa gtggtcagga cgcctgtctt cttcgcttcg gacatgagcg
     1501 acgtcgcccg gccgatcgcc atcgacggca cccgcgacat gatcgaccgc ggctaccacc
     1561 gcgaagccat gttctggata gcggcaacgc aaacccgctg ccagacaatc ctcaccgccg
     1621 aagcccctca ggaacttcac gatcgattca cgcccggcta tcgagcaatg ctctccgacc
     1681 tcggtatcgt tgcgtacgac aacctcagcc agcgctcaga agcggtccgc tcctacctta
     1741 ggtcgttgtg gacaaccgcc gaaacgatca tggaagcacc tccggcacct gcggacccac
     1801 aaacgtagcg cggggttacc ggcgctctac cttgttttcg agaccgcttc gatcgacgtt
     1861 aacgtgaccg gggtcatggc gcggagcgtg ttctggattt cggcgacggt gtctgtgtac
     1921 cgcatcagat cgtcgatctc ggcctcgggt gcatcggccg tgactttggc ccggaagatt
     1981 acgttcgagg cgccgtcacc cggtccgccg aaatccccgt tgacttcgac ctcgacccgc
     2041 tcgacgctga tgccccgctt gtcggcttcg cgatagatgt cgttgcagta gcaggtggcg
     2101 agggcaagga agagcaactc gccgccactc gcgctggagc cgagacccgt catcttcggc
     2161 ggaatgctga tcgagcgcgt gttgccgttc gtcgagatga cggcctggtg ctcgccgtat
     2221 gcattctgga cctgcgcact gaccttcacg tcgctccccc agccacaacc agatctaccg
     2281 atgaagcgaa ccaattacca gcggatgacc gagccgtcga tgcggcggac agccggaaag
     2341 gcaccgtgca gctcgtgctc cgggaacgga tacttcgccg ccaaggcctc gtcgatgtcg
     2401 accccgagac ccggctggtc gttgcaccag agcatgccgt cccgcacctc cggcgtgcca
     2461 ggaaacacct ctcgcgccgt gtcgttgaac aggtgcagct cctggatgcc gaagttggtg
     2521 ctcgagatgt cgagatgcag gttcgcggca tgcccgaccg gcgagacatc gcccggaccg
     2581 tgccaggccg tcctgacgcc gaagaactcc gcaaaggccg ccagcttccg ggctatcgag
     2641 aggccgccaa tcgcggagat gtggacccgg atgtagtcga tcagccgatc cttgatcagc
     2701 atctcccact cggtctggtt gacgaagagc tcgcccatcg cgatcggaat cgacgtcttc
     2761 tcccgcaacc gcgggaacca gccgatgtcc tccggcgcaa gcggatcctc gaggaagaag
     2821 agccggaact gctccagctc cttggccagc ccgatcgcct ggatcggcga gacgcgctca
     2881 tgaatgtcgt gcagcaactc aatctcatcg ccgagggtca tccgcaggtg ctcgaacagt
     2941 ttcggcaccg cccgcaggta cgacgactcg tgccacggcc gctggaccgg ctccaggtgc
     3001 gcgggccggt ttccgacgtg cgcctgcgcc ggcgtgccga cgtcggccgg ctcggagccg
     3061 ccttgcgcgc cataggtcga gtaaccggcg atctcaacct gcgccctgat gtgcgtgaag
     3121 ccgagcccca tcagcatccg ggcgtgatcc tcgacctcct gcacatcccg gccggaggcg
     3181 tgggtgtaga cggtcgctgc cgtgcggacc ttgccgccga gtaattggta gaccggcatc
     3241 cctgcccgct tgcccttgat gtcccagagc gcctggtcca ccccggagag ggcgttgttc
     3301 atgatcggcc cggtccgcca gtacgagctc aggtaaaggg tctggtagat gtcctcgatg
     3361 tcgttcgggt ctcgtccgat cagttgcggc ctgaggtagg tctcgagcgc ctcgacgacc
     3421 gccagcggcc gttgcataaa cgtcgcgcag ccgagcccgt agagccccgg ctcgctcgtc
     3481 tcgaccttga cgacgaccag ccggatcccc tccggcgcgg tacagatcgc gcggacgttg
     3541 gtgatgcgca ccggcggcat ggaagcgcac ggctcccacg ccgcgggcag tatcggttcg
     3601 ggaaggtcga ttccccgcat cgggtctact gctcgaccca gacggtctgg atgttgacga
     3661 attcgcgcat gccgtagctc gagagctcgc ggccataccc gctgcgcttg acgccgccga
     3721 atggcacctt ccagttcgag gtggtcatgc cgttgatgaa gacgccgccg gcttcgatct
     3781 tgcgggccag tttcttggcg cgctcgatat cctgcgtcca gatcgcgctg ccgagtccgt
     3841 acggcgagtc gttcgccgcc gcaatcgccg catcctcatc cggcacacgg gtgatcgcgg
     3901 cgaccgggcc gaagctctct tcgcggaaga gcgtcatgtc gggcgtgacg ttatcgacga
     3961 ccgtcggcgg gtagtagtaa cccttgccat ccggccgctc gccaccggtg accagatcgg
     4021 cgccggcatc catcgattcc ctgagctgcc gctcgattgt gtcgcgcagg tcggagcgcg
     4081 acatcggccc catcttgacc gttcggtcgg tcggatcgcc gtactgaatt gcctcggcct
     4141 tctgcttaag cagctcgacg aacttgtcgg ccaccttgtc gaccacaatg aagcgcttgg
     4201 ccgagatgca gacctgcccc gcgttctgga atcgggagcg ggcggcgtag tcggcggcct
     4261 tctcgagatc agcgtcttcc agcaccacga aggcgtcgga cccaccgagc tcgagcacgg
     4321 tcttcttgat gtgccggccg gccgcttcgg ccaccttgct gccgaccgca tcgctgccgg
     4381 tcagcgtcac cgccgcgatc cgggggtcgg cgatgacatc ctcgacccgg tcgcccggaa
     4441 taaccagggc ggtgatcgcg ccgtccggca gaccggcctc gcgggcgatg tcctgcatgg
     4501 cgagcgcgct gccgatgaca ttgctggcgt gcttgagaac gatcgtattg ccggcaacca
     4561 gtgccggcgc cgcgtgccgg atcagctgcc agatcgggta attccagggc atcactgcca
     4621 ggatcacgcc gagcggctcg aaggagacat aggcctcgcg agcggtggtc tcgacgattt
     4681 cctgctcgag catcttcggc ccgttatcgg cgtagaaatc gcagttgccg gcgctttttt
     4741 cgacctcggc ctcggcttca gggagcgtct tgcccatctc cctggtaatc agcccggcca
     4801 gcttgtcctt gttcgcgcgc aaggcggcgc caagccggcg gacaccctcc gctcgctcct
     4861 cgatcggcac atcccgccaa atttcgaacg cgtcgcgagc gtgctgaatc ttgcggtcga
     4921 cctcttctgg cgaatcaatc ggataagtcg cgttcacctc ttcggtggcg ggattaattg
     4981 acgtgatgct catgactgct gcggtgctca ttaccttgtc ctccggttgc tgctcgctac
     5041 cagttatgcg tctagcatat tgcaggatga ggtttttggt gttgggtttt gggttttggg
     5101 ttttgggtct tgacgagaat cgctccggac cccgagagga ccgacgaaga ggcggaaacc
     5161 cggactccaa aacctaaaac ctaatagagc tgcgtcttgt ccaggactgg gctcatctcg
     5221 tcgtagcggc ctgcgaggaa gagcggcagg ttgcggatga agatgtcggt gatgcgctcg
     5281 ttctctcgcg gggcgttggc cgagcagtgc ggcgagatga gggtgttcgg caggtcccag
     5341 agctgggagt tcggcggtag cggctcctgc tgaaacacat cgagcgccgc aaagccaatc
     5401 cgtccgtcgc gaatccgggc gatcatctcc tgctcatcaa cgacggcacc acgcccgatg
     5461 ttgatgaacc agacgcccgg cttaattgca tcgaattcag ccgcgccgat caagcgctcg
     5521 gtcccgccgg tgtgcggcgt gacgaccacc accacgtcag ccgtgctcag cgcctcgtgc
     5581 aggcgaccga tcggcaggta gtgatcgacg ccaagagctt cggctcgctc cgagccacct
     5641 gtccgcccga ccgctgtcac acgcatgtcg aaggcatggg ctacccgggc gatctgccgg
     5701 ccaatccggc ccggaccgac aatggtcatc tgctttccgg ccag
//
LOCUS       DATAOZ010000267         8668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3267471, whole genome shotgun sequence.
ACCESSION   DATAOZ010000267 DATAOZ010000000
VERSION     DATAOZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267471"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VHR64_07070"
     CDS             complement(<1..653)
                     /locus_tag="VHR64_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3302628.1"
                     /translation="MSILIQAASVAYAHGGNQIFTDVNFELQEGERVALIGENGSGKS
                     TLFRLLSRDISPDRGAVTHRRGLTIGFLTQDPSLDPGKTVDELLAAAVGDPEQMERGL
                     DTLAQRLVEPLDDDEMAAVLDEYNAGLARLDEFLAADMSSDVDEIIAALGIDSVRQQR
                     FGRLSGGEKKLVALARFSLLKPDVLLLDEPDNHLDADAKVWLEAHLASYRGAVGLIS"
     gene            867..1949
                     /locus_tag="VHR64_07075"
     CDS             867..1949
                     /locus_tag="VHR64_07075"
                     /inference="COORDINATES: protein motif:HMM:NF000689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX3302629.1"
                     /translation="MSIFGAQSIADPRFDDQAREYDALLVLSFGGPEGEADVIPFLEN
                     VTRGRNVPRERLLEVAKHYLHFGGVSPINAQNRALIAALETELKAHGPDLPIYFGNRN
                     WHPFVTDTIAQMRTDGVRRALVFVTSAFSTWSGCRQYREDVIRALEKLGPVGPDFDKI
                     RVFYNHPGFIEPMIEMTRQALDQVSPERRAGAEVIFTAHSVPTEMARHSAYVLQLNEA
                     ARLIAQGVGTARFRLAYQSRSGSPRIPWLEPDILDELSALHQQGVTDVVVVPLGFISD
                     HMEVLFDLDQEAVEHAGKLGMTMIRSATVGTHPTFVRMIRELIVERMSEHPDRRALGR
                     RGPNHDICPVSCCLLSSPATHSVSTR"
     gene            2203..2697
                     /locus_tag="VHR64_07080"
     CDS             2203..2697
                     /locus_tag="VHR64_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302630.1"
                     /translation="MDRRTVARGAGFAAALALLQQGGARAAGTPSAEGTPEPTLYDRL
                     GGIFAISAVVNRFSDQIIINPKLNVNPNLVAWNKNEAETRLPGLKFMRTLWVAALAGG
                     PFTYTGLPLHDAHTRFHLTSDEFDEVGAEIVRALQYYKVPEAETKLLVDAYMLSKPDV
                     VASE"
     gene            2975..3913
                     /gene="hemC"
                     /locus_tag="VHR64_07085"
     CDS             2975..3913
                     /gene="hemC"
                     /locus_tag="VHR64_07085"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX3302631.1"
                     /translation="MGSTAVVRIGARGSRLSRAQVEEVRLLLLGAWPGITVEVEIFVT
                     TGDIQLETPLPLMGGKGVFTSEIETALREGRIDFAVHSLKDLPVANPDGIVIGAVPER
                     ASVADVLVSRSGRGFHELSPNPVIGTSSYRRAAQLQSARSDSRTESIRGNVETRLRKA
                     MDPDGPYDAIVLAKAGLERLGLLDVITEELPLDLMLPAPGQAALAVQCRDDADSIVLA
                     AALDHLPTRLAVEAERAFLSGLGGGCSAPVASYGELHNGQLTLRGRVLALDGQHVIDV
                     QSGTGCGDVDSAQEAGRSLAATALQQGAGVLVEVAR"
     gene            3910..4707
                     /locus_tag="VHR64_07090"
     CDS             3910..4707
                     /locus_tag="VHR64_07090"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX3302632.1"
                     /translation="MTSPLVGRKIVNTRALEQAGELDDLLREQGAIPVSYPCIAIAPP
                     DDPAGLDAALDDLRAGKFDWLVLTSTNTVRSLAARRFHALEAPAWRTAVVGPATRDVA
                     LHELGLQADVMPEVYQGSDLAESLSVKPGDRVLLPQSAIAPPDLAGALTAKGATVRTV
                     TAYQTTIGNGGVRFAEILALGLVDAVVFASSSAVVGFVSRLTREGLSVAVLCNVPVVC
                     IGQSTASTARQRGFPSPVVPDEQTLGGVVGALESVFAHQPVSKGMIT"
     gene            4704..5756
                     /gene="hemB"
                     /locus_tag="VHR64_07095"
     CDS             4704..5756
                     /gene="hemB"
                     /locus_tag="VHR64_07095"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX3302633.1"
                     /translation="MISDLITPATPATGTAVFPAIRPRRLRRTPALRQLVRETRLTPS
                     DVIYPMFVRPGRDLHIPISSMPGQAQWSVDRLVGEVKAVAALGIPGVMLFGIPEHKDE
                     IGSDNWDPEGIVPQAIRAIKESVPEMVVISDMCLCEYTDHGHCGMINHPGHEHFNPAL
                     ENGYLLNDPTLELLARSSVAHAAAGADVIAPSGMIDGMVGAIRAALDQASFEHVAIMS
                     YAAKFASGFYGPFREAAESPPQFGDRSQYQMDPGNRREALKEAALDVAEGADVIMVKP
                     ALPYLDVLSDVRQSFNLPIAAYQVSGEYAMLQAAAANGWIDLDRCALESLTAIKRAGA
                     DMILTYFAKDVARWSQ"
     gene            5744..6820
                     /gene="hemE"
                     /locus_tag="VHR64_07100"
     CDS             5744..6820
                     /gene="hemE"
                     /locus_tag="VHR64_07100"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX3302634.1"
                     /translation="MESVATVPPEHPDSVHDSRFLRSCRREPVDATPIWLMRQAGRYM
                     AEYRTLRERYGILDLIKHPELACEVTLQPINAFDLDAAIIFADILPPLEGMGLSLEFV
                     RGEGPLIHNPVRAAADVERLRTPSPEDALPFTLEAIRLSRAELDPRGIPLIGFSGAPF
                     TLACYAVEGGASRHQAKVKQLMMSEPAAWDALMTKLSTVAGEYLLAQARAGAQALQLF
                     DSWCGELSPADYANYVLPHTRRTIEIAKSGDVPIILFGTGTGGLLELMATAGPDVIGV
                     DWRVCLGTAFDRLGPEIAVQGNLDPIALLATWDATRERAQIVLDQADGRPGHIFNLGH
                     GILPQTPVDQVRRLVDFVHQATRT"
     gene            6820..8247
                     /gene="hemG"
                     /locus_tag="VHR64_07105"
     CDS             6820..8247
                     /gene="hemG"
                     /locus_tag="VHR64_07105"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX3302635.1"
                     /translation="MERVDVAIIGAGIAGLALADRLHIQAPELTVRLFEASDRPGGKI
                     RTERIAFDDGRFIVESGPDAILAQKPWALDLIAELGLSDQLIPINRFPRSTMILKGGR
                     LVDLPDGVALIAPTKIWPFVRTDLLSWSGKIRAARDFVTGPRSGDGDESLGSFVTRRF
                     GREVLDWLAEPLAAGIYNADPSQLSLHATFPQLAQAEQKSGSVIRGLRASRKTAPAKP
                     STPTFLTLRDGMQTLTDALTERVGPILRTNAPATSILPLADEGYVVRVGTRDVRATNV
                     VLTVPAAQAAALVGVVAPEASRELSALRANHAGTISLAYRTTDIPRPLTGYGTVVPKR
                     EGRPINAITIASRKFDCRAPEGWTLIRVFFGGYRSPDTMRLSDGALRDLIAAELRSVM
                     GITGQPALDHIQRWPQGSPQYDVGHLDRIARIEAALPDGVALTGSPYRGIGIPDIVHQ
                     SWQLAGQLRKTAATAPVLAELKLFR"
     gene            8267..>8668
                     /locus_tag="VHR64_07110"
     CDS             8267..>8668
                     /locus_tag="VHR64_07110"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX3302636.1"
                     /translation="MTIADQTRPLTTTQPANAAKPATTFTVYWLFQADPNRRVATDTE
                     REHAALSMLDAIARHGDSVQVRGAYSTTGLSAGVDLILWLVADDCDAFQSLASEIHHS
                     SAGRALTLRHVYLGVGSMSQYDPTHSPAFLRG"
BASE COUNT         1530 a         2890 c         2707 g         1541 t
ORIGIN      
        1 tgcgagataa gtcccaccgc accacgataa ctagccaggt gggcctcgag ccaaaccttg
       61 gcatcggcat cgagatggtt gtccggctca tcgagcagca gcacgtcagg cttgagcaag
      121 gagaaccgcg ccagagcaac cagcttcttc tcgccgccgc tcagcctgcc gaatcgttgt
      181 tgtcggacgc tgtcaatgcc gagcgcggcg atgatctcgt cgacatcgga cgacatgtcc
      241 gccgccagga actcgtcgag tcgggcaagt ccggcgttgt actcgtcgag cacggccgcc
      301 atctcatcgt cgtcgagcgg ttcgaccagg cgctgggcga gcgtgtcgag cccgcgctcc
      361 atctgctcag gatcgccgac cgccgccgcc agcaactcat cgaccgtctt gcccggatcc
      421 aggctcggat cctgggtcag gaagccgatg gtcaatccgc gccggtgcgt aaccgcgccc
      481 cgatccgggg atatgtcgcg agagagcagc cggaacagcg tgctcttgcc cgagccgttc
      541 tcgccgatca aagcaacccg ctcgccctcc tgcaactcga agttgacgtc agtgaaaatc
      601 tggttgccgc catgcgcgta cgcgaccgag gcagcctgaa tcaggatgga catggcggtt
      661 cctgtcgaga agtcgagaag tcgagaagtc gagaagtcga gaagtcgaga agagattgta
      721 cgcggttggg atcgttgccc ggttcggggc cggcgggctc ggcgagatgc gtaatggtag
      781 tccacaatgg ttcttcgacg attcggcgct gggtggtctg accgattcgc cttgagctat
      841 gacaactccg gtgagaggta ttccagttga gcatttttgg tgcccagagc attgctgatc
      901 cgcggtttga tgaccaggcg cgcgagtacg atgcgctgct tgtgctttcg ttcggcggac
      961 ccgagggcga ggcggatgtc attcccttcc tcgagaacgt cacacgcggc cggaacgtgc
     1021 cgcgcgagcg gttgctggag gtcgcgaagc actatctgca ctttggtggc gtcagcccca
     1081 tcaacgccca gaatcgtgcc ctgatcgccg cgctggagac cgagttgaag gcacacggcc
     1141 cggacctgcc aatctacttc ggcaaccgca attggcaccc gttcgtgacc gacaccatcg
     1201 cccagatgcg gacggatggg gttcggcgcg cactggtctt cgtcacctca gccttcagca
     1261 cctggtcggg ctgccggcag tatcgcgagg atgtgatccg ggcgctggag aaactcggac
     1321 cggtcgggcc ggatttcgac aagatccgcg tcttctacaa ccaccctggc ttcatcgagc
     1381 cgatgatcga aatgacccgc caggcgctcg accaggtttc gcccgagcgc cgcgccggag
     1441 cggaggtcat cttcacggcc cacagcgtgc cgacggagat ggcgcggcac agcgcctacg
     1501 ttcttcaact caacgaagcg gctcgactca tcgcccaggg cgtcgggacc gcgcgctttc
     1561 ggctcgccta ccaaagccgg agcggatcgc cgcgaatccc ctggctcgag ccggacatcc
     1621 tcgacgagct gagtgctctg caccagcagg gcgtgaccga tgtcgtggtc gttccgctgg
     1681 gcttcatctc cgaccatatg gaggtcctct tcgatctgga ccaggaggcc gtcgagcacg
     1741 ctggcaagct gggcatgacg atgatcaggt cagccacagt cggcacgcat ccgacattcg
     1801 tgcggatgat ccgtgagctg atcgtcgagc gaatgtccga gcaccccgat cggcgcgccc
     1861 tcggccgccg cgggccgaac cacgatatct gcccggtcag ctgctgcctt ctcagctcgc
     1921 cagcaacgca ctcggtctca acccgatagc gccacccaag gtccaccgcg ctttacccgc
     1981 accaaatgcg ccgcgcgaac gagcgcttta cccataatgt caaataacat cttgacaaat
     2041 tggaaatacc tgctactgtg tactcggtct cagacctgga tctcaagatc tcggacggtc
     2101 accgctcctg agcgaagcgg tggtgagaga ccgttgtcgc cgaaatcccg ggcatcggag
     2161 cgccgggcca actgatcggg gcagatcgcg atggaaaatc ggatggatcg acggaccgtg
     2221 gcacgcggag caggattcgc cgcggcactc gcgctactac aacagggagg cgcacgggca
     2281 gccggtacgc cgtcagcaga ggggactccc gagccgacgc tttacgatcg actcggcgga
     2341 atttttgcca tttcagcggt ggtcaaccgc ttcagcgacc agatcatcat caaccccaag
     2401 ctcaacgtga accccaacct cgtggcatgg aacaaaaacg aagccgagac ccgcctgccc
     2461 ggcctgaagt tcatgcgcac gctctgggtc gcggcactgg ccggcggtcc gttcacgtat
     2521 accggattgc ccctgcacga cgcccacacg cgcttccacc tgacttcgga tgagtttgac
     2581 gaggtcgggg ccgagatcgt ccgggcactc cagtattaca aggtgccgga agccgagacc
     2641 aagttgctcg ttgacgcgta catgctcagc aaacctgacg tcgtggcatc cgagtagatc
     2701 gagcgtaccc cgggtcggtc gccgactggt cgccgccatg aacgatccag gcgcttctcc
     2761 atcaccttcc ccccttcctc aaggtcgtct cctggttctc cgggaccggg gacgacccca
     2821 tcgctccgga tgtagcagcc ctacaccaca tcccgcaccg attccggttt ctccatgcag
     2881 cgggtgctat tttgcggttg ggtgcactga tgtacccaga tttgcccaga attctcggca
     2941 aatctcgcac cgcgcgggtg tcgacaggga accaatgggc agtacagcag ttgttcggat
     3001 aggcgcgcgg ggctccaggc tgtcgcgtgc gcaggtcgaa gaggtccggc tattgctcct
     3061 cggggcctgg cccggcatca cagtcgaggt tgagatcttc gttacgaccg gggatatcca
     3121 gctcgagaca ccgctgccgt tgatgggcgg taaaggcgtc ttcacgagcg agatcgaaac
     3181 cgcgttgcgc gagggccgga tcgatttcgc cgttcactcg cttaaggact taccggttgc
     3241 caatccggac gggatcgtga tcggcgcggt gcccgagcgg gcgtcagtgg ccgatgtcct
     3301 ggtttcccgc agcggccgtg gctttcatga actgtcgccg aacccggtga tcggcacgag
     3361 cagctaccgg cgtgccgccc agctccaatc ggcccgatcc gactcccgaa ccgaatcgat
     3421 ccgcggtaac gtcgagacgc gcctccgcaa ggcgatggat cccgacggcc catacgatgc
     3481 gatcgtgctg gcgaaggccg gtctcgagcg gctcggcttg ctcgatgtca tcaccgagga
     3541 gttgccgctc gacctgatgt tgcccgcgcc gggacaggct gcgctcgcgg tccagtgccg
     3601 ggacgacgct gattcgattg tgctcgccgc agcgctcgat cacctgccga cccgactggc
     3661 ggtcgaggcc gagcgtgcat tcctttccgg tctcggcggc ggctgctcgg cgccagtcgc
     3721 gtcctacggt gagctgcaca acggtcaact cacgttgcgc ggacgagtgc tcgcgctcga
     3781 tggtcagcac gtgatcgatg tccaatccgg gaccgggtgt ggggacgtcg attcggcgca
     3841 agaggcaggt cgatcactgg ccgctactgc gctccagcag ggcgccggcg tgctcgtgga
     3901 ggttgcgcgg tgaccagccc gctggtcgga cgaaagatcg tcaacacccg tgccctggaa
     3961 caggccggtg agctcgacga tttactgcgc gagcagggcg cgattccggt ctcgtatccg
     4021 tgcatcgcga tagcgccgcc ggacgatccg gccgggctcg atgccgcgct ggacgacctc
     4081 cgggctggca aattcgactg gctggtcctg acaagcacca acaccgttcg ctcacttgcc
     4141 gctcgacgct tccacgccct cgaagcgccg gcgtggcgaa cagcagtcgt gggaccagca
     4201 acccgcgatg tcgcactgca cgaactggga ttgcaggctg atgtcatgcc cgaggtctac
     4261 caagggtctg accttgcgga gtcgttatcg gtcaaaccgg gagaccgcgt gctcctgccc
     4321 caatcggcca tcgcgccgcc ggatctggcc ggggccttga cggcgaaggg cgcgacagtc
     4381 cgcaccgtga ccgcttacca gacgaccatc ggcaatggtg gagtccgctt tgccgagatc
     4441 ctggcgctcg gattagtcga tgcggtggtc ttcgccagct cgtctgccgt cgttggattc
     4501 gtatcccgac tgacacgaga gggtctcagc gtcgccgttc tatgcaacgt tcccgtggtc
     4561 tgcattggcc agagtacggc cagtaccgct cgccagcgtg gcttcccgtc gccagttgtc
     4621 cctgacgagc agacgctggg tggggtcgtc ggcgcgcttg aatctgtatt tgctcatcag
     4681 ccagtcagca aaggaatgat cacgtgatca gtgacctcat cacacctgcg acaccggcca
     4741 ccggcacagc cgtgttcccg gcgatccgcc cccgccggct ccgccggacc cctgcgctcc
     4801 gccaactcgt ccgcgagacc cgcttgacgc catccgatgt gatctacccg atgttcgtcc
     4861 ggccgggccg tgatctccac attccgatct cgtcaatgcc gggacaggcg cagtggtcgg
     4921 tcgatcgtct cgtgggcgag gtcaaggcgg tcgctgccct cggtattccg ggggtcatgc
     4981 tgttcggcat tcccgagcac aaggacgaga tcggcagcga caactgggat ccggaaggga
     5041 tagtgccgca ggccatccgc gcaatcaagg agagcgtgcc cgagatggtc gtcatctccg
     5101 acatgtgcct ctgcgagtac accgaccacg gccattgcgg catgatcaat cacccggggc
     5161 acgagcactt caacccggcc cttgagaacg gttacctgct caatgacccg accctcgagt
     5221 tgctggcccg ctcgtcggtc gcccatgccg cagccggggc ggatgtaatc gccccgtccg
     5281 ggatgatcga tggcatggtc ggcgcgatcc gggccgccct ggatcaggcc agtttcgagc
     5341 acgtcgcgat catgtcgtac gccgccaagt tcgcaagcgg cttctacggt ccattccgtg
     5401 aggcggccga gagcccgccg cagttcggcg atcgcagcca gtaccagatg gatcccggca
     5461 atcgccgcga ggcgctcaag gaagcggcgc tggacgtggc agagggcgcg gacgtgatca
     5521 tggtcaagcc cgccttgccc tacctcgatg tccttagcga tgtccggcag tcgttcaacc
     5581 ttccgatcgc cgcctaccag gtcagcggcg agtacgcgat gctccaggcc gcggccgcga
     5641 acggttggat cgacctcgat cgctgcgcgc tggaatcgct gaccgccatc aagcgggccg
     5701 gcgccgacat gatcctgacc tacttcgcca aggatgtcgc gcgatggagt cagtagcgac
     5761 cgttccgcca gagcaccccg attcagtcca tgacagccgg tttctgcgct cctgccgccg
     5821 ggagccggtc gacgcgacgc cgatctggct gatgcgccag gccggtcgct acatggccga
     5881 gtaccgcacg ctgcgggaac gctacggcat cctcgatctg atcaagcatc ccgaattggc
     5941 ctgcgaggtc acgctccagc cgattaacgc cttcgacctc gacgcggcga tcatcttcgc
     6001 cgacatcctg ccgccgctcg aagggatggg actctcgctc gaattcgtgc gcggcgaggg
     6061 cccgctcatc cacaacccgg tccgggccgc cgccgacgtc gagcggctgc ggacgccttc
     6121 gcccgaagat gcgctgccct tcacgctcga ggcgatccga ctgtcccgcg ccgagctcga
     6181 tccccgcggg attccgctga tcggtttcag cggcgcacct tttacgcttg cctgttacgc
     6241 cgtcgagggc ggcgccagtc gacaccaggc caaagtcaag cagctcatga tgagcgagcc
     6301 ggctgcctgg gacgcgctga tgacgaagct gagcaccgtg gccggcgaat acctgctcgc
     6361 ccaggcccgg gccggcgccc aggcacttca gctcttcgat agctggtgcg gcgagctcag
     6421 cccggccgat tacgccaact acgtcctgcc ccacactcgc cggacaatcg agatcgccaa
     6481 gagcggcgac gtaccgatca tcctcttcgg caccggcacc ggcgggctgc tcgagctgat
     6541 ggcaaccgct gggccggatg tgatcggcgt cgactggcgg gtctgcctcg gcaccgcctt
     6601 cgatcgtctc ggtccggaaa ttgcggtgca gggcaacctc gatccaatcg ctctcctggc
     6661 gacgtgggac gcaacccgcg agcgcgccca gatcgtgctc gatcaggccg atgggcgtcc
     6721 cgggcacatc ttcaatctgg gccacggcat cctcccgcaa acgccggtcg accaagtgcg
     6781 ccgactggtc gactttgtgc atcaggcgac gaggacatga tggaacgggt cgacgtcgcg
     6841 atcatcggcg cgggtatcgc cggtctggcg ctggccgacc gattgcacat ccaggcgccg
     6901 gagttgacgg tgcggctgtt cgaggcatcg gatcggcccg ggggcaagat tcggaccgag
     6961 cggattgcgt tcgacgacgg ccgctttatc gtcgagtccg gtccagacgc gattctggcc
     7021 cagaagccgt gggcgctcga tctgatagct gagctcgggc tgagcgatca gttgatcccg
     7081 atcaaccgat ttccgcgctc aaccatgatt ctgaaaggcg gtcgcctggt tgacctgccg
     7141 gacggcgttg cgctgatcgc cccgacgaag atctggccct tcgtgcggac cgatctgctc
     7201 tcatggtcgg gcaagatccg ggccgcgcgt gacttcgtga ccggtcctcg atcgggcgat
     7261 ggcgacgagt cactgggttc gttcgtgacg cggcgatttg ggcgcgaagt gctggattgg
     7321 ctggctgagc cgttggccgc cggcatctac aacgcggatc caagccaact cagcctgcat
     7381 gcgacctttc cacagcttgc gcaggccgag cagaaatcgg gaagcgtgat ccgcgggctc
     7441 cgcgcatcga ggaagactgc accggccaag ccatccacac ccacgttcct gacgctccgc
     7501 gatggcatgc agaccctgac ggacgccctc accgaacgtg tcggccccat tctgcgaacg
     7561 aacgcgccgg ccacctcgat cctgcccctc gccgacgagg gatatgtcgt tcgggtcggg
     7621 acgcgcgatg ttcgcgcaac caacgtcgtg ctgaccgtgc cggccgcaca ggctgccgcg
     7681 ctcgttggcg tagttgctcc cgaagcgtcg cgcgagctct cggcgctgcg ggcaaatcac
     7741 gccggaacga tctccctcgc ctatcggacg acagacattc cccgtccctt aaccggatat
     7801 ggaacggtgg tccccaaacg ggaaggacgg ccgatcaatg ccatcacgat cgcttcgcgc
     7861 aaattcgatt gccgcgcgcc tgaaggctgg accctgatcc gcgtcttctt cggcggctac
     7921 cgcagcccgg ataccatgcg cctgagcgac ggcgctctgc gcgacctcat cgccgcggag
     7981 ctacgatcag taatgggcat caccggccag ccggccctcg accacatcca gcgctggcca
     8041 caaggcagcc cgcaatacga tgtcggtcac ctcgaccgga tcgcgcggat cgaggcggca
     8101 ttgcccgacg gcgtcgcact gaccggcagc ccataccggg gcatcggaat tccggacatc
     8161 gtccaccagt cgtggcagct cgccggccag cttaggaaga cggcggctac ggcaccagtt
     8221 ttggccgaat tgaaactgtt tcgttgatag atacggaggc agcgatatga cgattgcaga
     8281 ccagacacgt cccctgacca cgacccagcc ggcgaacgcg gccaagccgg cgaccacctt
     8341 caccgtctac tggctatttc aggccgatcc aaaccggcga gtcgcaaccg acacagagcg
     8401 cgaacacgcc gctctgtcga tgctcgacgc gatcgcccgc catggcgact cggtccaggt
     8461 gcgcggcgcc tactcgacga ccgggctctc tgccggcgtc gacctgatcc tctggctggt
     8521 tgccgacgac tgcgacgcct tccagagcct ggccagcgag attcaccact cgtcggccgg
     8581 tcgggcgctc actctgcggc acgtctatct cggcgtaggt tcaatgtcgc agtacgaccc
     8641 aacccactcg cccgccttcc tgcgcggc
//
LOCUS       DATAOZ010000268         6715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4847634, whole genome shotgun sequence.
ACCESSION   DATAOZ010000268 DATAOZ010000000
VERSION     DATAOZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4847634"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2264
                     /locus_tag="VHR64_07115"
     CDS             <1..2264
                     /locus_tag="VHR64_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302637.1"
                     /translation="TDDAAASLIGRRLTRRQTLALLGGGLTGGAMLGAVTGDPGRSVD
                     AAQITIEAGPSEAQSTGRPTIADIAAALQWDTEQIFRYVADQVAYEPYVGALRGAEGT
                     LRAMAGNAVDQSLLLAALLTEAAVPYRFAIGQISAEGQTALEAQLAPTVEELSDRKLR
                     TFRNPTDPPDTIATPPALSDDDVATLERLKTTATGINATAGNRADALVSMLSDVIAKA
                     GIDLPEVTATPLPDREQREHVWIQVADGPFWIDLDPSVPTAAQKQTFAEVSTTVDALG
                     PELDHLVRVTIVVEEASGDAAVPRDVVSIERPSRELIDAPIGIGITTPSAFESLGITI
                     TNLFEGGETYVPLLVVGDEAVSADSPIGFGGNGGGILGGLSGGDDSIDGEALRLGYRL
                     EIVSPDADPVAVERTIFDRIDPVQRAAGSIDLTTIESMDGPAGDRVRAAINSFTMIDV
                     ACADVPPPFPLSRRQELFFGGLTYFSASFYSLRNSLGFDWGSRLGWHPVLTAPNAVAL
                     RFQPEDPEQADAPVLVEADLLHQARGWRQTETLAPAGVSPWIARGVVDQVAERLVLDP
                     GLWMTDAAASGIVPGMSAGLIFETAADKKVPIVALDPSAAQPADLAAYPDAAQVRITA
                     ALDAGMWVIVPGKPVAIDGQDALAWWLVDAATGRITDQLPDGRSGASATIREPRVFYS
                     DLTEYVEMLGNFIVKWRNAISCFGNVTGFLVGIAAWLVYAFGSSATATGTWLSYAGLW
                     AFYFRMIGSCAGV"
     gene            complement(2314..>6715)
                     /locus_tag="VHR64_07120"
     CDS             complement(2314..>6715)
                     /locus_tag="VHR64_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3302638.1"
                     /translation="PANYESEPNDQFTAASTMGAADTGFQGSGALIPSDHDYFRVSIT
                     GEPQLWQVIVEGSGFSRIEAIDASQRTIAYADRDQTSNTATIANLLLRPGDHWFAVSG
                     DSGDYSVRLVPLGPPPPNLEQEPNSTVDGAQRMLMGDERTGQLLDMIDEDVYRFSLLA
                     EEHVILRLDPPADGSVNMNLTWGVTQLVHLGGAEPGTPLTYDALLKPGDYIVTLDADT
                     PSSGIYKLALTRADPFDLPADLEPNDAPPEASPLQPNQTISGTAAHSTSRDDADWYVL
                     PAGRARPVVIELKTDPANYLSLDVTALGTASVPEAKLSMTPPPDGGPGGTVELPAGQA
                     IAIGIYGDGDYTVTVKDALSKRYAEPVALPVTVGLTVPETPVAAYWADAQQIDGTITL
                     TNTGTAPVDLTLDAMLSDYRWSVALGSDPITLAAGESRTIPAPITVGADAWADLPVRV
                     TVRARDAGGNFATAFAEITPNREAPPLNPQPGWTLAPELRGGLDVAWSGFGAVPMQPD
                     QRAYGYEVMLYDGISTNGSGWWEYRSRIGAEPLALTVDLAGDSPVPVAGFILNPFGDS
                     GDPSGGLSRFDVQLSTDGQTFETVSSGELSALPVDQAFALDAPVDARYARVLVYSNQL
                     SFGRDQVVIGEFKVIAEPGWSFPDDRAAIQPLPAPNSAPPTPVATSPDVPQASGLEIS
                     APERGGHVVFTQPQFGSPTEAEWLLQPGGGESIRFPSDDPQIVWVVGFQHERAAQVSS
                     IVWDGPEQVDPNWLPIGSVEISVSTESPNGPWQSIGTWDLGSADADLAISLDSPVWAR
                     YVRFTAMPTLPAEFQEGQSFSAFGPGATHIYERPTDDTYRSILGEWGQDSRMAIYEQL
                     VPAVAAETDADAGNSAAEATNLPIDQTHTDTVQTNKDEDWYAVQAPADSGILKFTLTG
                     APTVDVAVTLFDLDGNEVPLEADDGQRERTYQASVMPGATYRLRVVKPPTALVVAFDM
                     SGSMGAYLDMIFNGVGVYSEAVVPGLEVVNFLPFGEGPLLPEMSGDPLELQTAITNYP
                     RTASSSDSESSMLDALKALDGQPGTTAILLLTDAESGSYGSTTELWSALNQVQPRIFT
                     VQIGAGQDISSQQDLMQDWADANQGIYHYVGAQSDVDVAFDRTATVLRRPSLYSVSVE
                     AIALPPTPTPMPTNTPEPTATPSPSPTPSPTVPAEPGTIRVVPPKQQAEGPAPVAPDV
                     SVALILDTSGSMLQGLEGSTRAQVAKAALTNLVTYTLPPGTNVSLRSFGNTPDSCETR
                     LVVPRGPLDPGSMTAQIDALEVVNLVRTPIGASLEAVAGDLGAQPGPKIVVLVTDGEE
                     TCGGDPEAAIRALIAQGIDVHVNIVGFALDDQALKDQFKRWAKIGNGQYIDAANSAEL
                     SAAVTKVVQPLYRVIDANGDVVATGQVGGPSQRVPAGTYTIEVLTDPVQRYKNVEVKP
                     GERTSVRMKTPKRTSTTTLAATSPRRRQQI"
BASE COUNT         1028 a         2184 c         2367 g         1136 t
ORIGIN      
        1 cgaccgatga cgccgctgct tcactcatcg gtcggcgctt gacccggcgg cagaccctcg
       61 cactgctcgg cggcggactg accggcgggg ccatgctcgg cgcggtcacc ggcgatcccg
      121 gccgttctgt tgatgccgcc cagatcacga tcgaagccgg cccgtcggag gcgcagtcaa
      181 ccgggcgacc gaccattgcc gacattgccg ccgcgctcca gtgggacacc gagcagatct
      241 tccgctacgt ggcagaccag gtcgcctacg agccgtacgt cggggcgctg cgcggggccg
      301 agggcacact gcgggccatg gccgggaacg cggtcgacca gtcgctgctt ctggccgcgc
      361 tgctgacgga ggctgccgtg ccctatcggt tcgcgatcgg gcagatctcg gccgaggggc
      421 aaaccgcgct cgaagcgcag ctcgcgccga cggtcgaaga gctgagtgat cgaaaactgc
      481 gcacgttccg caacccgacc gacccgcccg acaccatcgc gacaccacct gcgctctcgg
      541 atgacgatgt cgccacgctg gagcggctga aaacgacggc caccgggatc aacgcaaccg
      601 caggcaaccg cgccgatgcg ctggtctcca tgttgagcga tgtcatcgcc aaggccggca
      661 tcgatcttcc cgaggtcacg gctaccccgc tgccggaccg cgagcagcga gagcacgtct
      721 ggattcaggt ggccgatggc ccattctgga tcgacctcga tccgagcgtg cccacggccg
      781 cacagaagca gacgtttgcc gaggtctcga caaccgtcga cgctctcggc cctgagctgg
      841 accacctcgt tcgcgtcacg atcgtcgtgg aagaggcatc cggtgatgcg gcggtcccgc
      901 gggacgtggt ctcgatcgag cggccgagcc gcgagctgat cgatgcgccg atcgggatcg
      961 gcatcacgac accctcggcc ttcgagtcgt tgggcatcac gattaccaac ctgttcgagg
     1021 gcggtgagac ctatgtgccc ctgctcgttg tcggcgatga ggccgtctct gccgactcgc
     1081 cgatcgggtt cggcggcaac ggcggcggca tcctcggcgg cctctccggc ggtgatgact
     1141 cgatcgatgg cgaagccctc agactcggct accggctcga gatcgtctcg cccgatgccg
     1201 atccggtcgc ggtcgagcga acgatcttcg accgaatcga cccggtccag cgtgcggcag
     1261 gctcaatcga cctgaccacg atcgaatcga tggatggacc cgccggggac cgggtacgag
     1321 ccgcgatcaa ttccttcacg atgatcgatg tggcatgcgc cgacgtgccg ccaccgtttc
     1381 cgctcagccg ccggcaggag ctcttctttg gcggcctgac ctatttcagc gcctcgttct
     1441 attccctccg gaacagcctc ggattcgact ggggctcgcg gcttggctgg cacccggtcc
     1501 tgaccgcgcc gaatgccgtt gcccttcgat tccagcccga agaccctgag caggcggatg
     1561 ctccggtcct ggtcgaagcg gacctgctcc accaggcgcg cggttggcgg cagaccgaga
     1621 ctctggcgcc ggccggggtc agcccgtgga tcgcacgggg cgtggtcgat caggtggccg
     1681 agcggttggt gctcgatccc ggcctgtgga tgacggacgc ggcggcatcg ggcatcgtgc
     1741 cgggcatgag cgccggcctg atcttcgaga cggcggcaga caagaaggtg ccgatcgtgg
     1801 ccctcgatcc atctgccgcg cagccggcgg acctggccgc ctatccggac gccgcccagg
     1861 ttcggatcac ggcggcgctg gatgccggca tgtgggtgat cgtgcccggg aaaccggtcg
     1921 cgatcgacgg gcaggatgcg ctggcctggt ggctggtcga tgcggcgacg ggccggatca
     1981 ccgaccagct cccggatggg cgatccgggg cgtcggcaac gatccgggag ccgcgcgtct
     2041 tctactcgga cctgaccgaa tacgtggaga tgctggggaa tttcatcgtc aagtggcgca
     2101 acgcgatcag ctgcttcggc aacgtgactg gattcctggt cggaattgcg gcgtggctcg
     2161 tctacgcgtt tggcagcagc gcgaccgcga cgggtacgtg gcttagctat gccggcctct
     2221 gggccttcta tttccgcatg atcggctcct gcgccggcgt ttgacgccgg gccagccatt
     2281 gcggatgccg gccacggggg gccggcgcta cgtctaaatt tgttgccgcc gccgtggcga
     2341 ggtggcggct aatgtcgtcg ttgaggtgcg cttcggagtc ttcatgcgga cgctggtgcg
     2401 ctcgcccggc ttgacctcga cgttcttgta gcgctggacc ggatcggtca acacctcgat
     2461 cgtgtaggtg ccggccggga cgcgctggct ggggccgccg acctggccgg tggcaacgac
     2521 gtcgccgttg gcatcgatga cgcggtagag cggctggacg accttggtga ccgcggccga
     2581 gagctccgcg ctgttggccg cgtcgatgta ctggccgttg ccgatcttcg cccagcgctt
     2641 gaactggtcc ttgagcgcct gatcgtcgag cgcgaagccc acgatgttga cgtgcacgtc
     2701 gatcccctgc gcgatcaggg cccggattgc cgcctcggga tcgccgccgc aggtctcctc
     2761 gccgtccgtg accagcacca cgatcttcgg ccccggctgc gcgccgagat caccggcgac
     2821 tgcttcgagc gacgcgccga tgggcgtgcg gaccaggttg acgacctcga gcgcgtcgat
     2881 ctgcgccgtc atcgatcccg ggtcgagcgg gccacggggg acgaccagcc gcgtctcgca
     2941 ggaatcgggc gtgttgccga aggagcgcag cgagacgttc gtgcccggcg gcagcgtgta
     3001 ggtgaccagg ttggtcagcg cggccttggc cacctgcgcc cgggtactgc cttcgagccc
     3061 ctgcagcatc gagccggagg tatcgaggat cagcgcgacc gagacgtccg gggcgaccgg
     3121 cgccggtccc tccgcctgct gcttcggcgg gacgacgcgg atcgtgcccg gctccgccgg
     3181 aaccgtcggc gacggagtgg gcgagggcga tggagtcgcg gtcggctccg gcgtgttcgt
     3241 cggcataggc gtcggtgtcg gcgggagtgc gatcgcctcg acgctgacgc tatagagcga
     3301 cggacggcgc aggaccgtgg cggtgcgatc gaaggcgaca tcgacatcgc tctgggcgcc
     3361 gacgtagtgg tagatgccct ggttggcgtc ggcccaatcc tgcatcaggt cctgctggga
     3421 cgagatgtcc tggccggcgc cgatctggac ggtgaagatg cgcggctgaa cctggttgag
     3481 cgcggaccag agctcagttg tggagccgta gctgccgctc tcggcatcgg tcaggagcag
     3541 gatcgccgtc gttcccggct ggccgtccag cgctttcagg gcgtccagca tgctcgattc
     3601 cgagtcgctg gaggaggctg tgcgcgggta gttcgtgatc gcggtctgga gctcgagcgg
     3661 atctccgctc atctccggca gcagcggtcc ctcgccgaag ggcaggaagt tgaccacctc
     3721 gagcccgggc acgaccgcct cggagtagac accgacgccg ttgaagatca tgtcgaggta
     3781 ggcgcccatg ctgccgctca tgtcgaaggc gacgaccagc gcggtcggtg gcttgaccac
     3841 gcgcaggcga taggttgcgc cgggcatcac cgatgcctgg taggtccgct cgcgctggcc
     3901 gtcatccgcc tcgagcggaa cctcgttgcc atccaggtcg aacagggtaa cggcaacgtc
     3961 caccgtcggc gcgccggtca gggtgaactt caggattccg gaatcggccg gcgcctgcac
     4021 cgcgtaccaa tcctcatcct tgttggtctg gacggtatcg gtgtgggtct ggtcgatcgg
     4081 cagattcgtg gcctcggcgg cgctgttccc ggcatcggca tcggtctctg ccgcgaccgc
     4141 cgggacgagt tgctcgtaga tcgccatgcg cgagtcctga ccccattcgc ccaggattga
     4201 gcgataggtg tcatcggtcg gccgctcgta gatatgggtt gcgccgggac cgaaggccga
     4261 gaacgactgg ccttcctgaa attcggcggg caatgtgggc atcgccgtga accggacgta
     4321 ccgcgcccag accggggaat cgagcgagat cgcgaggtcg gcatccgcgg aacccagatc
     4381 ccacgtgccg atgctctgcc acggaccatt cgggctctcg gtactgaccg aaatctccac
     4441 cgagccgatc ggcagccagt tgggatcgac ctgctccgga ccgtcccaga cgatcgagct
     4501 cacctgggcc gctcgctcat gctggaagcc gacgacccag acgatctgcg ggtcatcgga
     4561 cgggaaccgg atcgactcgc caccacccgg ctgaagaagc cactcagcct cggtcggaga
     4621 gccgaactgc ggctgcgtga agacgacgtg accgccgcgt tcgggcgccg agatttcgag
     4681 cccggacgcc tgcggcacgt ccggcgaggt cgcgaccggt gtcggtgggg cggaattggg
     4741 cgcgggtaac ggctggatcg cggcacggtc gtccgggaag ctccagcccg gctcggcgat
     4801 caccttgaac tcgccgatca cgacctgatc ccgcccaaat gagagctgat tggaatagac
     4861 cagcacgcgg gcgtacctgg catcgaccgg cgcatccagc gcgaacgcct gatcgaccgg
     4921 cagggccgag agctcgcccg aagagaccgt ctcgaatgtc tggccatcgg tcgagagctg
     4981 cacatcgaac cggctcaatc caccagaggg atcgccggaa tcgccgaacg ggttgaggat
     5041 gaagccggca accgggaccg ggctatcccc ggccaggtcg accgtgagtg ccagcggctc
     5101 ggcgccgatc ctcgatcggt actcccacca gccgctgcca ttggtcgaga tgccgtcgta
     5161 gagcatgacc tcgtagccgt atgcgcgctg atccggctgc atcggaaccg cgccgaagcc
     5221 actccaggca acgtccagcc cgccgcggag ctcgggtgcc agggtccagc ccggttgcgg
     5281 gttgaggggc ggcgcctcgc gattcggcgt gatctcggca aacgcagttg cgaaattccc
     5341 gccggcatcc cgcgcgcgga cggtcacgcg cacgggcagg tcggcccagg catcggcgcc
     5401 gaccgtgatc ggggcaggga tggtccgcga ctcgccggcg gcgagcgtga tcgggtccga
     5461 tccgagcgcc acggaccatc ggtagtcgct cagcatggcg tccagcgtca ggtcgaccgg
     5521 cgctgtcccg gtattggtga gtgtgatcgt gccatcgatt tgctgggcgt cggcccaata
     5581 ggccgcgacg ggtgtttcgg gaaccgtgag cccgacggtg accgggagcg cgacgggttc
     5641 cgcgtagcgc ttcgacaggg cgtccttcac ggtcacggtg tagtcgccgt ccccgtagat
     5701 tccgatcgcg atcgcttgcc cggcggggag ttcgaccgtg ccgcccgggc cgccatcggg
     5761 gggcggcgtc atgctcagct tcgcttccgg cacgctggcg gtgccaagtg ccgtcacgtc
     5821 gaggctcagg taattggcgg ggtccgtctt gagctcgatc acgaccggtc gggcccggcc
     5881 ggcgggcaac acgtaccagt cggcgtcatc gcgggatgtg ctgtgcgcgg cggttcccga
     5941 gatggtctgg ttcggctgca gcggcgaggc ctcgggcggg gcgtcgttcg gctcgagatc
     6001 ggcggggaga tcgaacggat cggcccgggt cagggcgagt ttgtaaatgc cgctgctcgg
     6061 cgtgtcggca tcgagcgtga cgatgtaatc gcccggcttg aggagcgcat cgtaggtgag
     6121 gggcgtgccc ggttcggctc cgccgaggtg aacgagctgg gtcacacccc aggtcaggtt
     6181 catgttgacc gaaccgtccg ccggcgggtc gagccgcagg atcacgtgct cttcggccag
     6241 cagcgagaac cggtagacgt cctcatcgat catgtcgagg agttggccgg tgcgttcatc
     6301 gcccatcagc atccgctgag cgccgtcgac cgtactattc ggctcttgct ccaggttggg
     6361 cggcggcgga ccgagcggga cgagccggac cgagtagtcc ccgctgtcac cgctgaccgc
     6421 gaaccagtgg tcgccggggc ggagcagcag gttggcgatc gttgccgtgt tggaggtctg
     6481 gtcgcggtcc gcgtaggcga ttgttcgttg cgaagcatcg atggcctcga tgcggctgaa
     6541 tccgctaccc tcgacgatca cttgccagag ctggggctcg ccggtgatcg agacccggaa
     6601 gtagtcgtga tcgctgggaa tgagcgcgcc cgagccctgg aacccggtgt cggcggcgcc
     6661 catggtcgac gcagcggtga actggtcgtt cggctcgctt tcgtaattgg ccggc
//
LOCUS       DATAOZ010000269         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8530796, whole genome shotgun sequence.
ACCESSION   DATAOZ010000269 DATAOZ010000000
VERSION     DATAOZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8530796"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VHR64_07125"
     CDS             <1..377
                     /locus_tag="VHR64_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3302639.1"
                     /translation="TFVDEGPSMADLLKAATRRGIAPGYAHKLLAAFGSTGERVPVMQ
                     DMLDPLSERELDVLRLLGTDLTGPEIANELMVSLNTMRTHTKNIYAKLGVTSRRAAVR
                     LAEERGLVRRAGTNESGPVPDA"
     gene            531..806
                     /locus_tag="VHR64_07130"
     CDS             531..806
                     /locus_tag="VHR64_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302640.1"
                     /translation="MTTEHVSTNGPDTPGRYEIRLKGRLDARWAAWFDGLSLSHESDG
                     TTVLSGPVTDQAALHGVLGKVRDLGLPLISITQVESDHLDVSTDQPR"
     gene            837..1613
                     /locus_tag="VHR64_07135"
     CDS             837..1613
                     /locus_tag="VHR64_07135"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX3302641.1"
                     /translation="MTTAIAATTTKGIDMESANSAARWTGILYIITFITSIPALLLFG
                     PVLNDADYVVRGGSDNRIFLGAFLELILIIANIGSALAPYVILKRFSHRIALGYVGAR
                     IMESVFIGVGILAVLSVVTLRQDFAGAGNVDSATLIPVADALVAVKDWTFLFGPGFCG
                     IGNGIMLGYMMYRSRLMPRSLTWFGLVGGPLMVTAGVLALFGVLEPSGVAQFIMTIPE
                     IIWELSIGIFLTVKGASALKAASRDAHDTGEGAALELAAA"
     gene            complement(1681..2130)
                     /locus_tag="VHR64_07140"
     CDS             complement(1681..2130)
                     /locus_tag="VHR64_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3302642.1"
                     /translation="MLRFDHVGVVFDDLDAAISFFLDLGFEQEGRTTVEGEAVDTVNG
                     LAGVRAELAMVRTPDGTGRLELIKYHAPADTESSHPWPPNRPGLRHICIEVNDLTTLV
                     DRLRAKGFDTVGEVYDYQEYAYRLCYLRGPEGLIIELAEKITSGEAS"
     gene            complement(2220..>2951)
                     /locus_tag="VHR64_07145"
     CDS             complement(2220..>2951)
                     /locus_tag="VHR64_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302643.1"
                     /translation="PVTITAKRGGTLCQPTVVRNFNTSTPTPTNTPTDNPTNTPTNTP
                     TDTPTSTPTNTPTNTPTATNTPTATATPAVCGSGGPCTVFVTSSLYDGNLGGLAGGDA
                     KCQAHAVAAGLPGTYRAWLSGNALHASSRFDPSAGPYRLVNGTTIATNLSDLTDGALA
                     APIHVTESGDFIDGSAQTWVWTATQSDGTTFGQTCSNSASPADWSTTSARTIAGVSTA
                     ANASWTSTAIEPCTALLHLYCFQVA"
BASE COUNT          515 a          934 c          948 g          554 t
ORIGIN      
        1 ggactttcgt ggacgaaggt ccgtccatgg cggacctgct gaaggcggcc accagacggg
       61 ggatcgcgcc cggctacgcc cacaaactcc tggctgcgtt tggctcgacc ggggagagag
      121 taccggtcat gcaggacatg cttgacccgt tgagcgaacg cgagctcgac gtgctccggt
      181 tactcgggac cgatctgact ggtccggaaa ttgccaacga gctgatggta tcgctgaaca
      241 ccatgcggac ccacacgaaa aacatctacg ccaagctcgg cgtgaccagt cgacgggccg
      301 cggtccgcct ggccgaggag cgtggccttg tgcggagagc cggcacgaac gaatccgggc
      361 ccgttcccga cgcgtaaatc cccatccacc cgttcaaaaa atcaccacat caatcaccac
      421 ccgtggtgat gcgtgctcac cagatcggct cttaccttca gatcagacca acggacgatc
      481 gctacgccgg tgggaatcga tccgaagaag ccagtaaggg aaggagcgcc atgaccacag
      541 aacacgtttc gaccaacggt cctgacacgc cgggcaggta cgagatccgc cttaagggcc
      601 gtctcgacgc ccgctgggcc gcctggttcg acgggctgag cctcagccac gagagcgacg
      661 gcaccaccgt cctctccggc ccggtcaccg accaggccgc cctgcacgga gttctcggca
      721 aagtgcgcga cctcggcctg ccactgatct cgatcacgca ggtcgaatca gatcacctgg
      781 atgtatcgac cgaccaacct cgctagggac gcataacgtc acgaaaggac accagtatga
      841 cgaccgcgat tgcagcgaca acgacgaagg gaatcgacat ggaatcagcc aattcggccg
      901 cgcgatggac cggcattctc tacatcatca ccttcatcac ttcgatcccc gcgctcctgc
      961 tctttgggcc ggtgctcaac gacgctgact acgtcgtgcg cggcggctcc gacaaccgta
     1021 tcttcctcgg tgcgttcctc gaattgatcc tcatcatcgc caatatcggc agtgccctcg
     1081 caccgtacgt gatcctcaag cggttcagtc atcgcatcgc gctcggctac gttggggccc
     1141 ggatcatgga gtccgtcttc atcggcgtcg gcatcctcgc cgtcctctcg gtcgtgacgt
     1201 tgcgccagga cttcgcggga gccggcaatg tggattccgc aacgctgatc cctgtggctg
     1261 atgcactggt cgccgtcaag gattggacgt tcctgtttgg ccccggcttt tgcggcatcg
     1321 gcaacggcat catgcttggg tacatgatgt accggtctcg cctgatgccg cggtcactga
     1381 cctggttcgg actcgttggc ggtccgctga tggtcaccgc gggggtcctg gcgctgttcg
     1441 gtgtcctgga gccgagcgga gtggcccagt tcatcatgac catcccggag atcatctggg
     1501 agctgtcgat cggcatcttc ctgaccgtca agggcgccag cgcactcaag gctgcctcga
     1561 gggacgcgca tgacaccggt gaaggcgccg ccctggagct cgccgctgcc tgacatgcct
     1621 cgacagccat agtcccgaat gccaaggggg tgggtcggaa tgacccgccc cctcttcgtg
     1681 ctagctcgct tccccggagg tgatcttctc ggcgagctcg atgatgaggc cctcgggacc
     1741 gcgcagatag cagagccggt aggcgtactc ctggtagtcg tagacctcgc cgacggtgtc
     1801 gaagcccttg gcgcggagcc tgtcgacgag cgtggtgaga tcgttgacct cgatgcagat
     1861 atgccgcaag cccggccggt tcggcggcca gggatgcgag ctttcggtgt cggccggcgc
     1921 gtggtatttg atgagttcca gccggccggt gccgtccggc gtccgcacca tcgccaactc
     1981 cgcccgcacc ccggcgagcc cgttgaccgt gtcgaccgcc tcgccctcga cggtcgttct
     2041 gccctcttgc tcgaatccaa ggtcgaggaa gaacgagatc gcggcgtcaa ggtcatcgaa
     2101 gacgacaccc acatgatcga atcgcaacat gttcacacct caccggctga tcgctgtcgg
     2161 tcgggatggt cgcacgagac tgctgtggcg cgtcacaacg acacctcccg accgcgtccc
     2221 taggctacct ggaagcagta gagatggaga agagccgtgc atggttcgat cgccgtggat
     2281 gtccagctgg cattcgctgc tgtgctgact ccggcgatgg tccgcgccga ggtggtggac
     2341 cagtcggctg gagacgcgga attcgaacac gtctgcccaa aagtcgtccc gtccgactgt
     2401 gttgccgtcc agacccaggt ctgggccgac ccgtcgatga agtcgcccga ctccgtgacg
     2461 tggatcggcg cggcgagggc gccgtcggtg agatcgctca agttggtcgc gatcgtggtg
     2521 ccattcacga gacgataggg accggcggac ggatcgaaac ggctgctcgc atgaagcgcg
     2581 ttcccggaga gccatgctcg gtaggtgccg ggcagacccg ccgcgaccgc gtgggcctgg
     2641 catttggcat cgccacccgc gaggccgccg agattgccat cgtagaggga gctggtgacg
     2701 aagacggtgc acggcccacc ggacccgcac acggcaggag tggcggtggc cgtcggcgtg
     2761 ttcgtcgctg tcggtgtgtt cgtgggtgta ttcgtcggcg tgctggttgg cgtatccgtt
     2821 ggtgtgttcg tcggcgtatt ggttgggttg tcggtggggg tgttggtcgg ggtcggcgtc
     2881 gaggtattga agttccggac caccgtcggt tggcacagtg tcccgccccg cttggccgta
     2941 atggtgaccg g
//
LOCUS       DATAOZ010000270         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4319800, whole genome shotgun sequence.
ACCESSION   DATAOZ010000270 DATAOZ010000000
VERSION     DATAOZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4319800"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..701)
                     /locus_tag="VHR64_07150"
     CDS             complement(189..701)
                     /locus_tag="VHR64_07150"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX3302644.1"
                     /translation="MAEKSHSRPVRVVFVCLGNICRSPIAERVAERAAAEAGMTGVEF
                     SSAGTISEEYDDPMDRRAAAVLREHGYRYADHRARRITAAQIESADLVIAMEDSHVRS
                     VLALATDPSKVSLLTDFDPAAEPGSGVPDPWFGSHAGFYDTLTAIESAMPGLLDRVRE
                     LQRSELPGSH"
     gene            complement(694..1071)
                     /locus_tag="VHR64_07155"
     CDS             complement(694..1071)
                     /locus_tag="VHR64_07155"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX3302645.1"
                     /translation="MRLVVNAAALAVATGILSGITLTGSSTVSKIITLLIVALIFGVL
                     NAIIKPLFALFTAPLILLTLGLFLIVINACMLLLTSWLAGLFDLGWEVDGFWTAVLGA
                     IIISIVSFILNVFLPDPDDKRRG"
     gene            complement(1191..1958)
                     /locus_tag="VHR64_07160"
     CDS             complement(1191..1958)
                     /locus_tag="VHR64_07160"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF014613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HEX3302646.1"
                     /translation="MTILILGGTAEARELAAALVEDGLDVVSSLAGRVNSPSLPSGRV
                     RIGGFGGVDGLAEYLRRQQVSAVIDATHPFAATIGANALRAANRTSTPLVRLERPGWG
                     GHSRSGSWTWVADGAAARAAADVACRPFLTTGRKLLPDFRPWADREVLVRLVEPPTAP
                     LPQRWILIMSRGPYRYLGERQILTEHGIDVLITKDSGGTHTVAKLDAAGDLGIPVVII
                     ARPQRPQVTHLETVAEAVGWCRSVSLLRRSASRGSRL"
     gene            complement(1955..>2796)
                     /gene="cbiB"
                     /locus_tag="VHR64_07165"
     CDS             complement(1955..>2796)
                     /gene="cbiB"
                     /locus_tag="VHR64_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEX3302647.1"
                     /translation="GAAASWLEARCYADRQTAGLLHTSALVGAAIGLGAALERLISHR
                     WWATLLTTAAATWAVLGGRSLSREAATVAEQLDGGRLVAARDQVRHLVGRDTAELSPE
                     EVARATVESVAENASDAIVAPLVWGSVAGLPGLLGYRAVNTLDAMIGHRSPRYLRFGW
                     AAARLDDLANWVPARLAALAAAIWAPLVGGTAKSALAVVRRDAGRHPSPNAGVVEAAF
                     AGALDVRLGGRNAYRGSVEDRGMLGAGRPVAVADIARASRLATAASLSALAVAVLLRG
                     LRS"
BASE COUNT          513 a          937 c          913 g          433 t
ORIGIN      
        1 tgtagtgctt tcatgagttc ctccccgagt tcccctctgg cgagcttttc agcgggccga
       61 tgagaaaccg gtgagaagct cgacatagga atttgtcgac ttgtcaatac ccagttgccc
      121 gaagttttga caccgcccgc ggaaaatcgg gctgaggagc gcgactttgc cgacgcccgc
      181 gatctggtct agtgggatcc gggcagctca gatctttgga gttcgcgtac gcggtcaagc
      241 aggccgggca tggcggattc gattgcggtg agggtgtcgt agaagccggc gtgcgatccg
      301 aaccaggggt ccggcacacc agagccgggc tcggccgccg gatcgaagtc ggtcagcagg
      361 ctcaccttgc tcggatcggt cgcgagcgcc aagacgctgc gtacatggct gtcctccatc
      421 gcgatcacga ggtcggccga ttcgatctgg gctgcagtga tcctgcgcgc cctatggtcg
      481 gcgtatcggt atccgtgctc cctgagtacc gctgccgcgc gacggtccat cggatcgtca
      541 tactcctcag agatcgtgcc ggcgctggag aactcgactc cggtcatgcc tgcctcagca
      601 gcggcgcgct ccgccacccg ttccgctata ggcgaacggc agatgtttcc caggcagacg
      661 aagacaacgc gtacgggacg ggaatgcgat ttctcagcca cgtctcttgt cgtccggatc
      721 gggcaagaag acgttcagga taaagctcac gatcgagatg atgatggcgc cgagcaccgc
      781 cgtccagaat ccgtcgacct cccagccgag atcgaacaag ccggccagcc atgaggtgag
      841 caggagcatg caggcattga tcacgatcaa gaacaggccc agggtcagca gtatgagtgg
      901 tgcggtgaag agcgcgaaga gcggcttgat gatcgcgttc agcacaccga agatcaacgc
      961 cacaatcaac aacgtgatga tcttgctaac ggtgctcgag ccggtcaggg tgatgccgct
     1021 caagatccca gttgcgaccg ccagcgctgc ggcgttcacc accagtctca cgatgaacct
     1081 catgggaccg atcgtcccac gaaacgcaaa tcctcggatc gcgccccgct gctcggagga
     1141 cgagcgagga gacacgctct tcgtctccga gcgaggacga cgtcagtggg tcacaagcgc
     1201 gatccgcgcg acgccgagcg tcgcaacagg ctcacggagc gacaccatcc gacggcctcg
     1261 gcgaccgtct ccagatgagt gacttgtggc cgctgcggcc gggcgatgat caccaccggg
     1321 atgccgagat cgcccgcggc atcgagtttg gccacggtgt gggtcccgcc cgagtccttg
     1381 gtgataagca cgtcgatgcc atgctctgtg aggatctgac gctcgcctag atagcgatac
     1441 ggcccacgcg acatgatcag gatccagcgc tgcggcagtg gggcagtggg cggctcgacc
     1501 agccgaacca gcacctcccg atcggcccag ggccgaaaat ctggcagcaa tttccgtcct
     1561 gtggtgagga acgggcggca ggctacgtcg gcggcggctc gcgcggcagc gccatcagcc
     1621 acccaggtcc aggagccaga gcgcgagtgt ccgccccagc ccgggcgctc cagccggacc
     1681 agcggcgtgc tggtgcgatt cgccgcccgc agcgcgttcg cgccgatcgt cgccgcgaac
     1741 ggatgggtcg cgtcaatcac cgcggagacc tgctggcgtc gcaggtactc ggcgagacca
     1801 tcgacaccgc cgaagccgcc aatccggacg cgtcccgatg gcaggctcgg gctgttgacc
     1861 cgaccagcga gcgacgagac cacatcaagg ccgtcctcga ccagagccgc tgccagctct
     1921 cgggcctcgg cggtaccgcc caagatcagg atcgtcatga tcgcagcccg cgaagcagga
     1981 ccgccacggc caatgcgctc agcgatgcgg ccgtcgcaag gcggctggcc cgggcgatgt
     2041 cggcgaccgc aacgggccgg ccggcgccga gcatgccgcg atcttcgaca ctcccgcggt
     2101 acgcgttccg tccgccgagc cggacgtcca gcgcgccggc aaatgccgcc tcgacaacgc
     2161 cggcattcgg gctgggatgg cggccggcgt cgcggcgtac gacggccagc gcacttttcg
     2221 cggtgccgcc gaccagcggc gcccagattg cggccgccag cgcggccagt cgggccggga
     2281 cccagttggc cagatcgtcg agccgtgcgg ccgcccagcc gaaccgcagg tagcgcggcg
     2341 agcggtgccc gatcatggca tccaaggtgt tgacggcccg atatcccagt agtccgggca
     2401 gacccgcgac tgatccccac accagcggag caacgatcgc gtccgaagcg ttctctgcaa
     2461 ccgactccac ggtcgctctg gccacctcct cgggtgacag ttcggcagta tcgcggccca
     2521 ccagatggcg cacttgatct ctggcagcga ccagccttcc gccgtccaac tgctcggcaa
     2581 ctgtggcggc ctcacgactc agtgaacggc cgccgaggac ggcccaggtg gccgctgcgg
     2641 tcgtcaggag ggtggcccac cagcgatgtg agatcagccg ctccagtgct gcgcctaacc
     2701 cgatcgcggc tccgaccaat gcgctcgtgt gcagcaaacc agcagtctgc cgatcggcgt
     2761 agcagcgggc ctccagccag gatgcggctg cgccga
//
LOCUS       DATAOZ010000271        10911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_115677, whole genome shotgun sequence.
ACCESSION   DATAOZ010000271 DATAOZ010000000
VERSION     DATAOZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10911
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115677"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VHR64_07170"
     CDS             <1..1209
                     /locus_tag="VHR64_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302648.1"
                     /translation="LLWWASVLATIACLERPHGAFIPIGVATWYLLTGRLRIDRQPIR
                     SLAAIGLIPAVAMLLLYTVINGLPSQQGLFLSEMEAAGPTESWLLIKRLAVIEIAYTG
                     FFVLPLIIGVGIGVWRALGNLPTYAIRLVIGAVAVTAAGIAWFWNQGRLMPYIPHFFG
                     RGGPGSGDLQHSRPPLFGPTVFEWLTIACGVAAICALILFLRSVAIPKRFGRSGIGLL
                     TCLLGWQAIGVLPQSFLFRNWIISLDRYLLPMLPFVVVIAVWAVDDREIMPVPAWPAM
                     AVVALFSIAGTRDVLVFQSDVWQIAHALNTAGVPNTRLDAGYAWDAYHLWEFSYQNHL
                     EPRTANGPWWVMSYAQATDSTYVIAGAPIQGYVVISAHEYSSWLHERPMYLYVMRRLD
                     APPDGVTWPP"
     gene            complement(1210..3363)
                     /gene="glgP"
                     /locus_tag="VHR64_07175"
     CDS             complement(1210..3363)
                     /gene="glgP"
                     /locus_tag="VHR64_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX3302649.1"
                     /translation="MDVTTDNDLNLPERIKGLEQLAYNLWWSWNPEARYLFRRIDRFV
                     WERVGENAVLFLYRVDPERLQWASFDAEFLKAYDSVMARFETYLDSEDQTWVAKNAPD
                     LRGKIAAYFSAEFGLYRSLPIYSGGLGVLAGDHIKEASDLGLAVCAVSLLYRQGYLSQ
                     RLTPDGWQQDVTPNLEPHSEPTTQMLDEDGKPILIDLALEDPDHPIKLAIWKVRVGRV
                     QLFLLDSDVEGNPEWTRTVSSRLYGGDREHRLRQEMILGIGGVRALRALGCEPAYWHA
                     NEGHAAFSLLERLREHVVAGETFEHAKKLVAATTVFTTHTPVPAGHDHFSPELMDRYF
                     GHFWPQLGISRDAFYALGSFDQTGNDFNMTAVSMRLADYRNGVSKKHGEVTRQMWHGI
                     WPERPIEEVPIRHITNGVHLQTWIAPRIARMLDAHLGPEWRDYQADADLWRGVRGIPD
                     DVFWQAHSGRKRRLLDVLSERLRQRWVNGGDSGQVLSGGPFLEEKVLTLGFARRFATY
                     KRATLLFHDPDRLAAILNNPDRPVQIVFSGKAHPADDGGKHLIRELIWRSRDPKFGGR
                     IAFAEDYDMGVASLLVSGVDVWLNNPQSPMEASGTSGMKASANGVPNLSILDGWWIEG
                     WRPDNSNGWGIVGSSATGWEQDNEDALAIYDALENEVAPLYYSRDERGLPRDWIRVCK
                     NAMMTIAPEFSSRRMVFDYIEHLYTPAAQGSLVTV"
     gene            3560..5293
                     /locus_tag="VHR64_07180"
     CDS             3560..5293
                     /locus_tag="VHR64_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX3302650.1"
                     /translation="MAVEVRTRNAWDLVVAGLAAEGVKYVFGLPGDPRHLYDALETTD
                     VPDAPKAIGVRYETSGAFMAMAYARATGHLAACFGCPGPGIANLVPGILEAYSGCTPM
                     LVLGVRASRQTDGMGAFQETDHIGMLRPITKWATTVETPEKVAWTIRRAVHLARTGKP
                     GPVYVELPADVAFSDLDGVEYQAAPTVAPPAPNAEAIRAAADLIEKAAKPLIIVGGGA
                     YLSGAGSAIGAFASTYGIAIQTTPAGRSTVEETHPLFVGLTGLYRTTVPREIFEESDL
                     IITAGSRMEEFQGGFLPRPTEPKIIQIDIDSYEIGRNWLPDVAIESDCRLAIEALHAE
                     LARRNHSPLVDRIAEVHSRRNAAIADAAADATAPVDNGEMPLAGKSVVHMINEVFGDK
                     TILVKENGGQDLWVYYWPYYQVLEQNCCVAPAEQTAMGYGVIGAMAVKLGRPDHYVVS
                     TAGDGAFQMSIHELGTAAQISTPVTWVVLDDGAFGWVQWIQRRDLDNRIVATDFEPHV
                     DLVKIAEASGCSAERLTDSRDLRAALERAKAANDAGKPHVLVVPIDQSQHHAEFDRFH
                     DFEPAPNSATA"
     gene            complement(5279..5929)
                     /locus_tag="VHR64_07185"
     CDS             complement(5279..5929)
                     /locus_tag="VHR64_07185"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX3302651.1"
                     /translation="MTEYRHFTPDELGQPATYHLLNAVIAPRPISWISTVSKDGVFNL
                     APHSYTTVASQDPPIVLFVSIGRKDSIRNIEETGCFVYNVGSHALVEHINRTSADFPP
                     EISEFEWAGVTPIPSTRIAAPRVAEAPVQMETTLFDIYRVGTTSNAIVMGEVVALHIT
                     DELFDGDRVETARLDPVGRMAGSLYAAMGEVFSLKRPTWRGLLEAGESPMEPSSGR"
     gene            complement(5926..6993)
                     /locus_tag="VHR64_07190"
     CDS             complement(5926..6993)
                     /locus_tag="VHR64_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3302652.1"
                     /translation="MDFVTEGFHHVTMVSRDAQRTVSFYRDLLGLRLIKKTVNFDLPT
                     TYHLYFGDATGSPGTILTFFEWPDAKRGSYGVGGIHHIALKTADDDTLLKWKRRLTDH
                     GVPVSGPYNRGWFHSIYFRDPDGQVLEIATLGPGYDLDEPIASLGQQLKMPPEGQLRG
                     HRDEAGIAEQTWEEPVPEITPDMDLRMIHHVTGMTDNLVRADEFYHQALGMRLIKQSV
                     NQDDPDTLHYFWANYDGTRVAPASSMTLFGWPETARRASEGVGQTHHIAFRAKDSEQQ
                     EAWREHLLGLGFEVTPVMDRNYFQSIYFRTHDGLLVEIATDPPGFSVDEDTDALGSEV
                     KLPPWLESQRSEILAGLRPLT"
     gene            complement(7023..8021)
                     /locus_tag="VHR64_07195"
     CDS             complement(7023..8021)
                     /locus_tag="VHR64_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEX3302653.1"
                     /translation="MAAVSRPVPNLHLPSVTKRAPWQDRPETRVSNWISLWDFDEPID
                     AGLIGAPYSAASISASGAAGGPEAIRMAFRYNTTYTPDWDTDIKDLTVRDLGDIGGHL
                     TDVVTAHGNIEQAVRDALTISEPFIPVIVGGDHSITAPAVRGFAAANEGKKIGIINID
                     AHYDVRVMDSGPHNGTPFRQLVEGGVASGKNVVELGIHGFMNASYYRQWANEQGITTI
                     TGRDVHKRGMEACILEALGIAGDGTDVIYVSVDIDCLAYPWAAGTSAASPEGLSAWDL
                     LEAMFICGQHPNVAALDVVEIDPTRDVRDQTARSGCSVILTFLAGLYRRIYGDRGY"
     gene            complement(8022..8693)
                     /locus_tag="VHR64_07200"
     CDS             complement(8022..8693)
                     /locus_tag="VHR64_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX3302654.1"
                     /translation="MCQGLTGPTHDGQDEWFLDNSAIRSFIGEVQRLRTEETDPARVV
                     DLIEPAFKSLLADPTWLPDHFAEPSAESGMGSGIGMWLLYRAGDGNLAFSSLVVPPGA
                     STPVHDHLAWGLVGLYRGTQDEVVFTRTDDESIDGAATLETQQHNSLTPGDLYQLLPE
                     TDIHQVRTTSDVTSVSLHLLGNDNGCIVRHRFHPDQGRVEPFKSGWLNVPCADQASGA
                     SQGME"
     gene            complement(8699..9400)
                     /locus_tag="VHR64_07205"
     CDS             complement(8699..9400)
                     /locus_tag="VHR64_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302655.1"
                     /translation="MLDITDVHTHYGHIHALKGITIEVQQGEVVTLIGSNGAGKSTTL
                     KTISGLLHPTTGQITLNGNRISGMPAHKIVELGVVQSPEGRRVFPRMTILENLEMGAF
                     SKRKVDPADLDRVFQLFPRLKERIAQKAGTLSGGEQQMLAIGRALMAHPSLLLLDEPS
                     MGLSPILVEAIFAIIKEINEQGTTVLLVEQNALMALGVAHRGYVLQTGSIVLSDNAAS
                     LLQNDMVRKAYLGEE"
     gene            complement(9406..10323)
                     /locus_tag="VHR64_07210"
     CDS             complement(9406..10323)
                     /locus_tag="VHR64_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302656.1"
                     /translation="MALQVPEQAPAPQIEGPIALETRGVTKRFGGLTAVDHVDLKIPT
                     GAIQGLIGPNGAGKTTYFNIIAGIYELSDGEVIFEGAPIHSAGGNGSRRTNLRPDQIA
                     NRGISRTFQNIRLFANMTALENVLIGMHTRLHSSPLGALLKLRSVKIEERNARQKARE
                     LLQYVGLAGKEEATARNLSYGDQRRLEIARALASDPRLLLLDEPTAGMNPNETAQLTR
                     FIRQIRTDFNLTILLIEHDMKVVMGISDTVAVLDRGQKIADGPPIEVQRDPRVIEAYL
                     GTAGSAAAAAGIKERAEGNGAGVEGSSQV"
     gene            complement(10323..>10911)
                     /locus_tag="VHR64_07215"
     CDS             complement(10323..>10911)
                     /locus_tag="VHR64_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX3302657.1"
                     /translation="LLSLLMIRRLYNSRLGRTWAAMREDEIAAASMGVNLVKTKLWAF
                     ALGASFAGFAGSIYASAFTIVSPDQFEFSVSITILAMVILGGLGNVYGVIVGAILIGS
                     FDRILAEELTKPLNSFGDAIGVSWLANHSVTSDRFLVFGLALVLMMLFRPAGLFPSAR
                     RKAEMQPEDEATLRHENIQLYDTLESDEPAAGSRV"
BASE COUNT         1950 a         3482 c         3415 g         2064 t
ORIGIN      
        1 ctgctctggt gggcgtcggt gttggcgacg attgcctgcc tggaacgacc acacggcgcg
       61 ttcattccga tcggcgtcgc gacctggtat ctgctgaccg gtcgcctacg gatcgaccgt
      121 caaccgatac gctccctggc ggcaattgga ctcattccgg ctgtcgccat gctgctgctt
      181 tacaccgtca tcaacgggtt gccatcccag caagggctgt tcctgagcga gatggaggca
      241 gcaggtccga ccgagagctg gctgctgatc aagcggctgg ccgtgatcga gatcgcgtac
      301 acaggtttct tcgtcctgcc cctcatcatc ggcgtcggaa tcggcgtttg gcgggcactc
      361 ggtaaccttc cgacctatgc catccggctg gtgatcgggg cagtcgccgt gacggcggcc
      421 ggcatcgcct ggttctggaa ccagggccgg ttgatgccgt atatcccaca tttcttcggc
      481 cgcggcggcc ctggttcggg cgatctgcag cacagccggc cgccgctatt cggcccgacc
      541 gtctttgagt ggttaaccat cgcgtgcgga gtcgcggcga tctgcgccct gattctcttc
      601 ctgcgctcgg tcgcgatccc gaaacggttc ggtcgatcgg gtatcggatt gctgacctgc
      661 ctcctcggat ggcaggcaat tggcgtgctg ccacagtcgt tcctgttccg gaactggatc
      721 atctcgctgg accgttatct gcttccaatg ctgcccttcg tggtcgtgat cgcggtctgg
      781 gcggtcgacg atcgggagat catgccggtg ccggcgtggc cggcgatggc agtggtcgcc
      841 ctgttctcga ttgccggcac gcgcgatgtg ctggtgttcc agagcgatgt ctggcagatc
      901 gcccatgccc tgaacaccgc cggcgtaccg aacacacggc tcgatgcggg ttatgcctgg
      961 gatgcctacc acctctggga gttcagctac caaaaccatc tcgagccgcg aacagcaaac
     1021 gggccatggt gggtcatgtc ctatgcgcag gcgaccgatt caacatatgt gatcgccggc
     1081 gccccgatcc agggttacgt cgtcatctca gcgcacgagt attcctcgtg gctgcatgag
     1141 cgcccgatgt acctgtacgt gatgcgccgg ctcgacgcgc cacccgacgg cgtgacctgg
     1201 ccgccctgat cagaccgtga ccagcgatcc ttgggcggcc ggggtatata gatgctcgat
     1261 gtagtcgaac accatccggc gggagctgaa ctcgggcgcg atcgtcatca ttgcgttctt
     1321 gcaaacgcgg atccagtcgc gtggcaggcc acgctcgtcc cggctgtagt agagcggcgc
     1381 gacctcgttc tcgagcgcgt catagatcgc cagtgcgtct tcgttgtcct gctcccaacc
     1441 ggtcgctgag gaccccacga taccccagcc gttgctgttg tccgggcgcc agccttcgat
     1501 ccaccaaccg tcgaggatac tcaggttcgg cactccgttg gccgaggctt tcataccgct
     1561 cgtgccgctg gcttccatag gtgattgcgg gttgttcagc cagacatcga cgcccgaaac
     1621 caggagcgag gccacgccca tgtcgtagtc ctcggcgaag gcaatccggc caccgaactt
     1681 tggatcccgc gacctccaga tcagctcgcg gatcaagtgc ttgccgccgt catcggccgg
     1741 atgcgccttg cccgaaaaga cgatctgcac ggggcgatct ggattgttga ggatcgcggc
     1801 gaggcgatca gggtcatgga acaacaaggt cgcccgcttg taggtcgcga atcggcgcgc
     1861 gaagccgagc gtcagcacct tttcctcgag gaacgggcca ccggagagga cctggccgga
     1921 atcgccgcca ttgacccagc gctgccgaag gcgttcgctg agcacatcca gcaaccgtcg
     1981 cttccggcct gagtgtgcct gccaaaagac atcgtccggg atgccgcgca cgccgcgcca
     2041 gaggtcggca tctgcctggt aatcccgcca ttcggggccg aggtgcgcat cgagcatccg
     2101 ggcgatccgg ggcgcgatcc acgtctgcaa gtggacaccg ttcgtgatgt ggcggatcgg
     2161 cacctcctcg atcgggcgtt ccggccagat cccgtgccac atctggcgag tcacctcgcc
     2221 gtgcttcttg ctcacgccgt tccggtaatc ggcgaggcgc atcgagaccg cggtcatgtt
     2281 gaagtcattg ccggtctgat cgaagcttcc gagcgcgtag aaagcatccc ggctgatccc
     2341 gagctgcggc cagaaatggc cgaaataccg atccatcagc tccgggctga aatggtcgtg
     2401 gccagccggc accggcgtgt gcgtcgtgaa gacggtcgtc gccgcgacga gcttcttggc
     2461 gtgctcgaac gtttcgccgg cgacgacgtg ctcccgcagt cgctccaaca ggctgaaggc
     2521 cgcgtgaccc tcattcgcgt gccagtaggc cggctcgcag cccagcgcgc ggagcgcccg
     2581 aacgccgccg atgccgagga tcatctcctg ccgcagccga tgctcgcggt cgccaccgta
     2641 gagccgggac gataccgtgc gcgtccattc agggttgccc tcgacgtccg agtcgagcag
     2701 gaagagctgg acacgtccga cccgcacctt ccagatcgcg agcttgatgg ggtgatccgg
     2761 atcctccagc gcgagatcga tcaggatcgg cttgccgtcc tcatcgagca tttgcgtggt
     2821 tggttcggaa tgcggctcga ggttcggcgt cacgtcctgc tgccagccgt ccggagtcag
     2881 gcgctgactc aggtagcctt ggcgatagag cagggaaacg gcgcagactg ccaaccctag
     2941 gtcgctcgct tccttgatat ggtcgccagc gaggacgccg aggccgcccg aatagatcgg
     3001 gagcgagcgg tagagtccga actcggccga gaaataggct gcgattttgc cgcgcaaatc
     3061 gggagcgttt ttcgccaccc aggtctggtc ctcggagtcg agataggtct cgaaacgggc
     3121 cataaccgag tcgtaagcct tcaggaactc cgcatcaaat gacgcccact gcagccgctc
     3181 gggatcgacg cgatacagaa agagcaccgc attctcgccg acccgctccc agacgaatcg
     3241 atcgatccgg cgaaacaggt agcgcgcctc ggggttccag ctccaccaga ggttgtaggc
     3301 gagctgctcc agacccttga tccgctctgg caggttcagg tcattatccg ttgtgacgtc
     3361 caaactgagc tcctccggca ccgagcacgc ggcgcacgcc gatgcaccga gccttcttcg
     3421 ttgaagttcc ttccgggacc ttaggctaac acaccatttg tgcaattgaa cggcaattgc
     3481 cccattgaca ggagacgcct cccagcgtag gttcccatgg tctgatcagg ctcacgaaca
     3541 ctatccagga gctccgccaa tggctgtcga agttcgtact cgcaacgcgt gggatctcgt
     3601 cgtggccggc ctggccgccg agggcgtcaa atacgttttc ggcctgcccg gcgacccccg
     3661 tcacctctac gacgcgcttg agacgaccga cgttccggat gcaccgaaag cgatcggcgt
     3721 ccgctatgag acctccgggg ccttcatggc catggcctat gcccgcgcta ccggtcacct
     3781 cgcggcctgc ttcggctgtc cggggccagg catcgccaat ctcgtgcccg gtattctcga
     3841 ggcctactcc ggctgcacgc cgatgctggt gctcggcgtc cgcgccagtc gccagaccga
     3901 tgggatgggc gcattccagg aaaccgacca catcggcatg ctccgcccga tcaccaaatg
     3961 ggccaccacc gtcgagaccc cggagaaggt cgcctggacg atccggcgcg ccgtgcacct
     4021 cgcccgtacc ggtaagcccg gcccggtcta cgtcgagctg cccgccgacg tggcgttttc
     4081 ggatcttgac ggcgtcgagt accaggcggc gccgaccgtc gctccaccag cacccaacgc
     4141 ggaggcgatc cgggcggcgg ccgacctgat cgagaaggcg gccaaaccgc tgatcattgt
     4201 cggcggcggt gcctatctct ccggcgcagg cagcgcaatt ggcgcatttg cctccaccta
     4261 cggcatcgcg atccaaacga caccagccgg ccggagcacg gttgaggaga cgcatccgct
     4321 cttcgtggga ctgaccggtc tttaccggac gacagttccg cgcgagatct tcgaggaatc
     4381 agacttgatc atcaccgccg gctcgcgcat ggaggagttc cagggcggct tcctgccgcg
     4441 accgactgaa ccgaagatca ttcagatcga catcgactcg tacgagatcg gccgcaactg
     4501 gctgccggat gtcgcgatcg agtcagactg ccgtctggcg atcgaggcgc tccatgccga
     4561 gctggcccgg cgcaatcact cgccgctggt tgaccgaatc gccgaggttc actcgcgccg
     4621 caatgccgcc attgccgatg ccgcggccga tgcgaccgct ccggtcgaca acggcgagat
     4681 gccgctggcc ggcaaatcgg tcgtccacat gatcaacgag gtcttcggcg acaagacgat
     4741 tctggttaag gaaaacggcg gtcaggatct ctgggtctac tactggccct attaccaggt
     4801 gctggagcag aactgctgcg tcgccccggc cgagcagacc gcgatgggat acggcgtgat
     4861 cggcgcgatg gccgtcaagc tcggccgccc ggaccactat gtcgtcagca ccgccggcga
     4921 cggcgcattc caaatgtcga tccatgagct cggcacggcc gcgcagatca gcacgccggt
     4981 gacatgggtg gtcctcgacg acggcgcgtt cggatgggtg cagtggatcc agcgacgcga
     5041 tcttgataat cggatcgtgg cgaccgattt cgagccgcat gtcgacctgg tcaagatcgc
     5101 ggaggcgtcg ggttgcagcg cggaacgtct gaccgattcg cgggacctgc gcgcggcgtt
     5161 ggagcgggcc aaagcggcga acgatgccgg caagccgcac gtccttgtcg tgccgatcga
     5221 ccagtcccag caccatgccg agttcgatcg gttccacgac ttcgagcctg cgccgaactc
     5281 agcgaccgct tgacggctcc atcggtgact cgccggcctc gagcagaccg cgccaggtgg
     5341 gccgtttgag gctgaatacc tcgcccatcg cggcgtagag cgagccagcc atgcgcccaa
     5401 ccgggtcgag ccgggccgtc tcgacccggt cgccgtcaaa gagctcgtcg gtaatgtgga
     5461 gggcgacgac ctcgcccatc acgatcgcgt tgctcgtcgt gccgactcgg tagatgtcga
     5521 agagcgtcgt ctccatctgc accggggcct cagccacacg cggcgcggcg atacgcgtgc
     5581 tcgggatcgg cgtcacaccg gcccactcga actcgctgat ttccggcgga aagtcggccg
     5641 aggttcggtt gatgtgctcg accagcgcat ggctgccgac gttgtagacg aagcagccgg
     5701 tctcctcgat gttccggatc gagtcctttc ggccaatgct gacgaagagc acgatcggcg
     5761 gatcctgcga ggcgaccgtc gtataggagt gcggcgcgag gttgaagacg ccgtccttcg
     5821 acaccgtcga gatccaggag atcggtcggg gagcgatcac cgcgttcaac agatggtacg
     5881 tcgcgggctg cccaagctcg tcgggggtga agtggcgata ctcggtcacg tcagcggtcg
     5941 caggcccgcg agaatctcgg agcgttgcga ttccagccac ggcggcagct ttacttcgct
     6001 gccgagggca tccgtatcct catcgacgct gaacccggga ggatcggtcg cgatctcgac
     6061 caacaggccg tcgtgggtgc ggaagtagat gctctggaag taattccggt ccatcaccgg
     6121 cgtgacctcg aacccaagcc cgagcaaatg ctcgcgccag gcttcctgct gctctgagtc
     6181 tttcgcgcgg aacgcgatgt gatgggtctg gccgacgcct tcgctggcac gccgggccgt
     6241 ctctggccag ccaaacagcg tcatcgagct ggccggcgcg acccgcgtgc cgtcgtagtt
     6301 cgcccagaag tagtgcaggg tatcgggatc gtcctggttg accgattgct tgatcaaccg
     6361 cataccgagc gcctggtggt agaactcgtc cgccctgacg agattgtctg tcataccggt
     6421 cacgtggtgg atcatgcgca ggtccatatc cggcgtgatc tccgggaccg gctcctccca
     6481 cgtttgctcg gcgatgcccg cctcgtcgcg atgcccccgc aactggccct ccggcggcat
     6541 cttgagctgc tgaccgagcg acgcgattgg ctcgtcgagg tcgtagccgg ggccaagggt
     6601 tgcgatctcg aggacctgac cgtccgggtc gcggaagtaa atgctgtgga accatccccg
     6661 attatagggg cctgacaccg gcacgccgtg atcggtcaga cgccgcttcc acttgagcag
     6721 ggtgtcgtcg tcggccgtct tgagggcgat gtggtggatg ccgccgacgc catatgagcc
     6781 ccgcttcgca tcgggccact caaagaaggt caggatcgtg ccaggcgagc cggtcgcgtc
     6841 gccaaagtag aggtggtagg tcgtcggcag gtcgaagttg accgtctttt tgatcaatcg
     6901 tagaccgagc agatcgcggt agaacgaaac ggtgcgctgg gcatcgcgcg agaccatcgt
     6961 gacgtggtga aagccctcag tgacgaagtc catgcgcacc tccgtgctca acgcgctccc
     7021 ggctagtacc cccggtctcc gtagatgcgg cgatagagcc cggcaaggaa cgtcaggatg
     7081 accgagcaac cagagcgcgc ggtttgatcc cgcacatccc gggtcgggtc gatctcgaca
     7141 acatcgagcg cggcgacgtt cggatgctgg ccgcagatga acatcgcctc cagcagatcc
     7201 catgcgctca acccctcagg gctggcggcg ctagtcccgg cggcccatgg ataggcaaga
     7261 caatcgatat cgacgctgac gtagatcaca tccgtgccat cgccggcgat gccgagcgct
     7321 tcgagaatgc acgcctccat tccacgcttg tggacgtcgc ggccggtgat cgtggtgatc
     7381 ccctgctcgt ttgcccactg acggtagtag gaggcgttca tgaaaccatg gatgccgagt
     7441 tcgacaacgt tcttgccgct cgcgactccg ccctcgacga gttggcggaa gggcgtgccg
     7501 ttgtggggac ccgaatccat cacgcggaca tcgtaatggg catcaatatt gatgatgccg
     7561 atcttcttgc cttcgttcgc cgccgcaaag ccgcggactg ctggcgcggt gatcgagtgg
     7621 tcgccgccga cgatgaccgg gatgaatggc tcactgatcg tcagcgcatc ccggaccgcc
     7681 tgctcgatgt tcccgtgggc ggtaacgacg tcggtcaggt gaccgccgat gtcgccgagg
     7741 tcgcggacgg tcaggtcctt gatatcggtg tcccagtcag gtgtgtaggt cgtgttgtac
     7801 cggaacgcca tccgaattgc ttccggaccg ccggccgcgc ccgaggcgga gatcgacgcc
     7861 gcggaatagg gcgcgccgat caggccggca tcgatcggct cgtcgaaatc ccagagcgag
     7921 atccagttgc tgacgcgcgt ctcgggtcga tcctgccatg gcgcccgctt ggtaaccgac
     7981 ggcaggtgaa gattcggtac gggtcgggat acagctgcca tttactccat tccctgcgac
     8041 gcgccagatg cctgatccgc gcacggcaca ttcagccaac cggatttgaa cggctcgacg
     8101 cgcccttgat cgggatggaa tcggtgccga acgatgcagc cgttgtcgtt cccgagcaag
     8161 tgcagtgaga cggacgtcac gtcggacgtc gtccgcacct ggtgaatgtc ggtctcaggg
     8221 agcagctggt aaagatcgcc gggcgtcagc gagttgtgct gttgcgtctc gagcgtggca
     8281 gctccgtcaa tggactcatc gtccgtgcgc gtgaagacga cctcgtcctg cgtgccacga
     8341 tagagaccga caaggcccca ggcgaggtgg tcatggaccg gggtggacgc gcctggcggc
     8401 acgaccaggc tggagaaggc gaggttgcca tcgcctgcgc gatagagcag ccacatgcca
     8461 atcccgctgc ccataccgct ctcggccgat ggctcggcaa agtgatcggg cagccaggtc
     8521 gggtcggcca gcagcgactt gaacgcaggc tcgatcaaat cgacgacacg cgccggatcg
     8581 gtctcctccg tcctgagccg ctggacttcg ccgataaatg aacggatagc cgaattatcg
     8641 agaaaccact cgtcctgccc gtcgtgggtc ggccctgtga gtccctggca cacgttgctt
     8701 actcctcacc gagatacgcc ttccgaacca tgtcattctg caacaacgac gcggcattgt
     8761 cgctgagcac aattgacccg gtctgcagga catacccgcg gtgagccaca ccaagcgcca
     8821 tcagcgcatt ctgctcgacg agcagaacgg tcgttccctg ttcgttgatt tccttgatga
     8881 tcgcgaagat cgcctcgacc aaaatcgggg aaaggcccat cgacggctca tccagcaaca
     8941 gcagcgacgg gtgagccatg agcgcccgcc cgatcgcaag catctgctgt tcacctccgg
     9001 agagcgttcc ggccttctgg gcgattcgct ctttcaggcg aggaaacagt tggaagacgc
     9061 gatccaggtc cgcaggatcc accttgcgct tggagaatgc gcccatctcc aggttctcga
     9121 ggatcgtcat cctggggaaa acgcgacgcc cttccggtga ttgaacaacg cccagttcca
     9181 cgatcttgtg ggccggcatg ccgctgatcc ggtttccgtt cagcgtgatc tgaccagtgg
     9241 tggggtgaag caagccggag atcgtcttga gtgtcgtgct tttgccagca ccattcgagc
     9301 caatcagggt cacgacctcg ccctgctgga cctcgatcgt gatgcctttc aacgcgtgaa
     9361 tgtgcccgta atgagtatgg acatccgtga tgtcgagcat cgccgtcata cctggctgct
     9421 gccttccaca ccagctccgt tgccctctgc ccgctccttg atcccggcgg cggcggcggc
     9481 cgagccggcc gtgccgagat acgcctcaat gactcgtgga tcgcgctgaa cctcgatcgg
     9541 tggcccgtcg gcaatctttt ggccacgatc aagtacggca accgtgtcag aaatacccat
     9601 gacaactttc atgtcgtgtt cgatgagcag gatggtcaga ttgaagtcgg ttcggatctg
     9661 gcggatgaag cgcgtcagtt gcgcggtctc gttcgggttc atgccggccg tcggctcgtc
     9721 gagcagcagt agccggggat cgctggccag ggcgcgcgcg atttcgaggc ggcgctggtc
     9781 gccgtatgaa aggttcctgg cggttgcttc ctcttttccg gcgagcccaa cgtactggag
     9841 caactcccgc gccttttgac gcgcgtttcg ctcctcgatc ttcaccgagc gcagcttgag
     9901 cagcgctccc aacggcgacg aatgcagccg ggtatgcatc ccgatcagca cgttctccag
     9961 cgcagtcatg ttcgcaaaca ggcggatgtt ctgaaacgtt cgtgatatgc cccgattcgc
    10021 gatttggtcg ggacgcaggt tagttcggcg actgccattc cctcccgcgg agtgaatagg
    10081 agctccctcg aagatgacct cgccgtcgct cagttcatag attccggcga tgatgttgaa
    10141 gtaggtggtc ttgcccgctc cattggggcc gatcagcccc tggatcgcgc cggtcgggat
    10201 cttgagatcg acgtggtcga ctgctgtgag gccgccaaac cgcttggtga cgcctcgcgt
    10261 ttcaagcgca atgggcccct cgatctgggg agccggcgct tgctcaggta cctggagtgc
    10321 catcaaaccc tgctcccggc ggctggctca tcagactcca gggtgtcgta gagctggata
    10381 ttctcgtgtc tcagtgttgc ttcatcctct ggttgcatct ctgccttgcg ccgggcgctt
    10441 gggaatagtc cggccggccg gaacaacatc atcaggacca gcgcgagccc gaacaccagg
    10501 aaccggtccg aagtcacact gtggtttgcc agccagctaa caccaattgc atcgccaaag
    10561 ctgttcaacg gcttggtcaa ctcttcggcc agaatccggt caaacgatcc gatcaggata
    10621 gcgccgacga tcacgccgta cacgttcccg agcccgccga ggataaccat cgcgaggatc
    10681 gtgatcgaca cggagaattc aaattgatcc ggagagacga tggtaaacgc ggacgcatag
    10741 atcgatccgg caaacccggc gaacgacgcg ccaagggcaa acgcccacaa cttggtcttg
    10801 accaggttca cgcccatgct cgccgccgcg atttcgtctt cgcgcatggc ggcccaggtc
    10861 cggcccagtc gcgagttgta caggcggcga atcatcagga gcgacagcaa c
//
LOCUS       DATAOZ010000272         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3008510, whole genome shotgun sequence.
ACCESSION   DATAOZ010000272 DATAOZ010000000
VERSION     DATAOZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3008510"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VHR64_07220"
     CDS             <1..766
                     /locus_tag="VHR64_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX3302658.1"
                     /translation="PQTGPFYVEGAEPGDTLVVHLDSVEPNRDWGWGASIPGFGALAP
                     EYKTAMVTPPAPDRLFVWHIDKTRKTATLDMPNSKIGKVEVPIRPFFGTIGTAPGGKE
                     CISSLYPGPYGANMDFNEVVAGVTMHFPVFEPGALFMLGDGHSAQGDGEIDGAAIETS
                     FNVKFTVNVIKGKKINWPHLINDNEIMSIGSTRPLMDALRLACVDMVDWLVSDYGFDK
                     YDAVQLLGQTAHLYIANVVDPQFSVACALNKKYLPK"
     gene            848..>2076
                     /locus_tag="VHR64_07225"
     CDS             848..>2076
                     /locus_tag="VHR64_07225"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HEX3302659.1"
                     /translation="MINFLFGVLRQTWEILEEASVFLLFGFLLAGMLGILAPGRLLTR
                     LVGTGKFKSVLWGSVVGAPIPLCSCGVLPTALGLQKQGATPGATIAFLVATPETGVDN
                     ISLTYALTDPVMTVFRPIAGVATAIAAGLATNLFGAARSKATEPPLEVANTSAAGDER
                     CLHAGDPKGHDHAHSHDHHDDHVQGADSLPDRGASRTVVDTTRRIIRYGFVELLDDVS
                     WWLVLGIVLSAVAVVAIPAQLFDGVWGGGIASMVLMLVLSIPLYTCASCSTPMAAALA
                     LKGLSPGAVLVFLLAGPATNIGSLVVLLKMLGRHAVAAYLAAVVIMTLAAGLALDAIY
                     HAWGLDPRATFGTAAGFVPDLVKEAAAVLLTGLLILSMCRTHVPNEWLWLRDRAAQLT
                     GIAVTPRGLGFAAAAGAA"
BASE COUNT          354 a          679 c          650 g          393 t
ORIGIN      
        1 cccgcagacc ggcccgttct atgttgaagg cgctgaaccg ggcgacacgc tggtggtcca
       61 ccttgacagc gtcgagccca accgcgattg gggctggggc gcctccattc cgggtttcgg
      121 ggctcttgcg ccagagtaca aaactgcgat ggtgacaccg cctgctcccg accgtctgtt
      181 cgtttggcat atcgacaaga cccggaagac cgccacactc gacatgccga acagcaagat
      241 cggcaaggtc gaagttccga tccggccgtt cttcggcacg atcggcaccg ctccgggcgg
      301 taaggagtgc atcagctcgc tctaccctgg gccgtacggc gcgaacatgg acttcaacga
      361 ggtcgtcgcg ggcgtcacca tgcacttccc ggtgtttgag ccgggcgcgc tgttcatgct
      421 gggcgacggg cattccgccc agggcgacgg cgagatcgac ggtgcagcaa tcgagacgtc
      481 gttcaatgtc aagttcacgg tgaacgtgat caagggcaag aagataaact ggccgcatct
      541 gatcaacgac aatgaaatta tgtcgatcgg cagcacacgg cctctgatgg acgccctgcg
      601 gctcgcctgt gtggacatgg tcgactggct ggtcagcgac tatggctttg acaagtacga
      661 tgccgtccaa ttgcttggtc agacggctca cctctacatc gccaatgtgg tcgacccgca
      721 gttctctgtg gcctgcgcct tgaacaagaa atatctgccg aagtaacgca gccgcgccac
      781 cggcggcgtc cgcacaaggc gccgcgggac ccattcggcc tcggatgacc gcgaagacgg
      841 gaccataatg atcaactttc tgttcggtgt ccttcggcaa acctgggaaa tcctggaaga
      901 ggcttcggtc ttcctcctct tcggcttcct gctcgccggg atgctgggga tcctcgcgcc
      961 ggggcggctg ctgacccggc tggtcgggac tggaaagttc aagtccgtgc tctggggatc
     1021 ggtcgtcggc gcgcccatac ccttgtgctc ctgcggcgtc ctgccaacag cgctcgggct
     1081 gcaaaagcag ggagcgacgc caggcgccac catcgcgttc ctcgtcgcga cgccggaaac
     1141 cggggtcgac aacatcagcc tgacctacgc gctgaccgac ccggtcatga ccgttttccg
     1201 ccccatcgcc ggggtggcaa cggcgattgc cgccggactc gccacgaatc tgttcggcgc
     1261 cgcgcgctcg aaagcgaccg agccgccgct ggaggtcgcc aacacttcgg ccgcaggcga
     1321 tgagcgatgt ctgcatgcag gcgaccccaa aggacatgat cacgcgcaca gccacgatca
     1381 ccacgacgac catgtccagg gggcagattc gctgccggac cgtggcgcca gcaggaccgt
     1441 agttgatacg acaaggcgca tcatccgtta cggtttcgtc gagctgctcg acgacgtgag
     1501 ctggtggctc gttctgggta tcgttttgtc cgctgtcgcc gtcgtcgcaa ttccagcgca
     1561 gctgtttgac ggggtgtggg gcggcggaat cgcctcgatg gtgctgatgc tcgtcctgag
     1621 catccctctt tacacttgcg cttcctgctc gacgcccatg gcggccgcgc tggctctcaa
     1681 aggcttgagc ccaggcgccg ttctcgtttt ccttctggcc gggccggcca cgaacatcgg
     1741 cagcctcgtc gtgctgctga agatgctggg ccgccacgca gtggctgcct atctggccgc
     1801 ggtcgtcatt atgacgctcg ccgcgggtct cgcactcgac gcgatttacc acgcctgggg
     1861 cctcgacccg cgcgcgacat tcgggacggc cgccggattc gtgccggacc ttgtcaagga
     1921 agctgccgca gtgctgctta ccggcctgct gatcctcagc atgtgccgga cgcacgttcc
     1981 aaacgaatgg ctctggctcc gcgaccgagc cgcgcagctg actggaatcg ccgttacccc
     2041 acgcggtctg gggttcgcag ccgccgccgg cgcggc
//
LOCUS       DATAOZ010000273         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1168209, whole genome shotgun sequence.
ACCESSION   DATAOZ010000273 DATAOZ010000000
VERSION     DATAOZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168209"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VHR64_07230"
     CDS             <1..879
                     /locus_tag="VHR64_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX3302660.1"
                     /translation="DFMKPDRIVIGTDTDRGAEVMRELYAPFNRNHQRMLVMDVRSAE
                     FTKYAANAMLATKISFMNELANIAERVGVDIESVRIGIGSDPRIGYAFIYPGCGYGGS
                     CFPKDVRSLERAAAEVGYDAQLLRAVEDVNDRQKQVLFSKISDYFDGNLAGKQIAVWG
                     LAFKPNTDDMREAPSRTLIEALWEAGATVVAFDPEAMHEAHRLYPIEISDGRLRLVEC
                     EDDVLDGSDCLVICTEWRSFRAPDFQTLRERLKNPVIFDGRNIHDPERVEREGFTYFG
                     IGLGQLPKPAMVGAAG"
     gene            882..1889
                     /locus_tag="VHR64_07235"
     CDS             882..1889
                     /locus_tag="VHR64_07235"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEX3302661.1"
                     /translation="MRILVTGAAGFIGSFVAERLLADGHSVLGIDDLNPYYSPSLKQD
                     RLARLEGREGFEFRQIAVQDRDAMRALFDGDRFDRVIHLAAQAGVRYSLENPEAYVDS
                     NLVGFLTILEGCRHHQVPHLVYASTSSVYGANTRQPFSPHDGVDHPVSLYAATKRANE
                     LMAHTYSHLYRLPTTGLRFFTVYGPWGRPDMAAWLFTKAILAGEPIQVFNHGKMRRDF
                     TYVTDVAEAVVRIADVVPEPNPAWDSDCPDPATSNAPFRIYNVGNSEPVELNHFIEVL
                     ERLLGKTAIRENLPLQPGDVLSTSADTSDLEAAINFRPQTSIEDGLAQFVAWYREYWQ
                     V"
     gene            complement(1918..2472)
                     /locus_tag="VHR64_07240"
     CDS             complement(1918..2472)
                     /locus_tag="VHR64_07240"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX3302662.1"
                     /translation="MTPQPAAWGRIELIVADITTLHVDAIVNAASSSLLGGGGVDGAI
                     HRSAGPELLRACREAGGCPTGEARITPGFRLPARWVIHAVGPVWRGGKQGESDQLASC
                     YREALRLASNPDFKIRSIAFPSISTGVYGYPLSNAAEIAITEIDRFLAQSDYLQRVIL
                     CAYSNSAYETYLDVAGSILGRHHE"
BASE COUNT          469 a          819 c          843 g          456 t
ORIGIN      
        1 gacttcatga agccggaccg gatcgtcatc gggactgaca ctgatcgcgg cgccgaggtg
       61 atgcgggagc tctacgcgcc gttcaaccgc aaccaccagc ggatgctggt catggacgta
      121 cggtcggccg agttcacgaa gtacgccgcg aatgcgatgc tggcgaccaa gatctcgttc
      181 atgaacgagc tggccaacat tgccgagcgg gtcggcgttg atatcgagag cgtgcggatc
      241 ggaatcggtt cagatccgcg catcggttac gccttcattt acccgggctg cggctatggc
      301 ggctcctgtt tccccaagga tgtgcgctcg ctggagcgag ccgccgccga ggtcggctac
      361 gacgcgcagc tcctgcgcgc ggtcgaggac gtcaacgatc gccagaagca ggtgctgttt
      421 agcaagatca gcgactactt cgacggcaat ctggccggaa agcagatcgc ggtctggggc
      481 ctggccttca agccgaatac cgacgacatg cgcgaggcgc cgagccggac cttgatcgag
      541 gcgctctggg aggccggcgc gaccgtggtc gcgttcgatc cggaggcgat gcacgaggcg
      601 caccggttgt acccgatcga gatcagcgat ggccggctgc ggctggtgga gtgcgaagac
      661 gacgtgctcg acggcagcga ttgtctggtg atctgcaccg aatggcgatc cttccgcgcg
      721 cccgatttcc agaccctgcg cgagcggctc aagaatccgg tcatcttcga cggccgcaat
      781 atccacgatc cggaacgggt cgagcgcgag ggcttcacct acttcgggat cggactcggg
      841 cagctgccga agccggcgat ggtcggcgcg gccggctagc gatgcggatc ctcgtcaccg
      901 gagcagccgg ctttatcggc tcgttcgtgg ccgagcgact cctcgccgac gggcattcgg
      961 tgctggggat cgacgatctc aacccgtact actcgccatc gctgaagcag gatcgcttgg
     1021 cgcggctcga gggtcgggaa ggattcgagt tccggcagat cgccgtccag gatcgggacg
     1081 cgatgcgggc gctgttcgat ggggaccggt tcgaccgtgt catccacctc gccgcccagg
     1141 ccggggtgcg ctactcgctc gagaacccgg aggcgtatgt cgacagcaac ctggtcggtt
     1201 tcctgacgat ccttgagggc tgccggcacc accaggtccc gcaccttgtc tacgcctcga
     1261 ccagctcggt ctacggcgcc aacacccggc agcccttttc gccccacgac ggggtcgatc
     1321 acccggtcag cctctacgcc gcgaccaagc gggcgaacga gctgatggcc cacacctaca
     1381 gccacctcta ccggctgcca acgaccgggc tgcgcttctt cacggtctac gggccgtggg
     1441 gccggccaga tatggcggcg tggctcttca cgaaggcgat cctggccggc gaaccgatcc
     1501 aggtcttcaa ccatggcaag atgcggcggg actttaccta tgtcacggac gtggccgagg
     1561 cagtcgtgcg aatcgcggat gtggtcccgg agccgaatcc ggcatgggac agcgactgcc
     1621 cggatccggc gaccagcaac gcgccgttcc ggatctacaa cgttggcaac agcgagccgg
     1681 tcgagctcaa ccacttcatc gaggtgctgg agcggctgct cggcaagact gcaatccgcg
     1741 aaaacctgcc gctgcaaccg ggcgacgtgc tctcaacgtc ggccgatacc agcgacctcg
     1801 aagccgcgat caacttccgc ccgcaaacca gcatcgaaga cggcctcgcc cagttcgtcg
     1861 cgtggtatcg cgagtattgg caagtctgat actgccgcgt ccgcttcgtc actcgcgtca
     1921 ttcgtgatgc cgtccaagga tgcttccagc tacatcgaga taggtctcgt acgcgctgtt
     1981 gctgtaggca cagaggatga cgcgctgcag gtaatcggat tgcgcgagaa atcgatcgat
     2041 ctccgtgatc gcgatctcgg cggcattgct gagcgggtat ccgtatacgc cggtgctgat
     2101 cgaggggaaa gcgatggagc gaatcttgaa gtcggggttc gacgcgaggc gaagagcctc
     2161 gcgatagcat gaggcaagct gatcgctttc cccctgtttg ccgcctcgcc aaaccggccc
     2221 gaccgcatgg atcacccatc gggcaggtaa tcgaaagccg ggtgtgatgc gcgcctctcc
     2281 ggtcgggcag ccaccagcct cacgacaggc ccgaagaagc tccggtccgg cggagcgatg
     2341 gatagcacca tcgacaccac ccccgcccaa caggctgcta ctagcggcat taacgatggc
     2401 gtcgacatgg agagtcgtga tgtctgcgac gatcagctcg atccggcccc aggctgcagg
     2461 ttgcggggtc atgcctcgct cgatcccgtt tcgctctctt gcaggctgat ctcgaggcga
     2521 agggtgtcgg attcgccggg gttgaggacg cggaggccgc tgtcgtagcc acgctcttcg
     2581 acgtcga
//
LOCUS       DATAOZ010000274         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_640491, whole genome shotgun sequence.
ACCESSION   DATAOZ010000274 DATAOZ010000000
VERSION     DATAOZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640491"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VHR64_07245"
     rRNA            complement(<1..133)
                     /locus_tag="VHR64_07245"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(732..1160)
                     /locus_tag="VHR64_07250"
     CDS             complement(732..1160)
                     /locus_tag="VHR64_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX3302663.1"
                     /translation="MNVQTILGDKGTDVATITGNATVADAVRVLGERRIGALPVLEGD
                     EVAGIMSERDVIYCLREHGAEALDWPVSRVMSAPAVTVEPATPVLAALAMITQRRIRH
                     LPVVSGGRLVGIVSIGDLVKHRIERIEEEAAAMREYIQTA"
     gene            1229..1513
                     /locus_tag="VHR64_07255"
     CDS             1229..1513
                     /locus_tag="VHR64_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302664.1"
                     /translation="MPSPDPLPSPRLSLLRRFRRIMRWMALFSIVVASIAVLLVARGD
                     THVPVHMLIATALGAGLSVLLAGALMSLVFLSNDSGHDEKANRFEEEDRP"
     gene            1510..1905
                     /locus_tag="VHR64_07260"
     CDS             1510..1905
                     /locus_tag="VHR64_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX3302665.1"
                     /translation="MTVTNRDPVLRVVPGPSDINANGHIFGGWVLSQMDIAGGITASR
                     RANGPVATVAIERMEFITPIHLRDLISVYAEVERVGRTSMGVRIEVIASRDRGATELK
                     VTEGLFTFVALDENHRPRAVEPQPSASSE"
     gene            complement(1881..2573)
                     /gene="phoB"
                     /locus_tag="VHR64_07265"
     CDS             complement(1881..2573)
                     /gene="phoB"
                     /locus_tag="VHR64_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX3302666.1"
                     /translation="MTRKKLLLVEDDRALAELLIWHFEREDFDVRRTGDGEEALLLAS
                     EDAPDVIILDWMIEGILGLEVCRRLRRRSETANVPIIMLTARGEEGDRIRGLETGADD
                     YVTKPFSPRELVARVGAVLRRVRPALAAEQLTYDDVEMDVGAHRVRRDGHPISLGPTE
                     FRLLKHFLENPGRVFSRERLLDSVWSHDPDIDLRTVDVHVRRLRKALNDGGKRDIIRT
                     VRSAGYSLDAEG"
BASE COUNT          495 a          893 c          803 g          502 t
ORIGIN      
        1 tattccgaac cagagggcag attcccacgc gttacgcacc cgtgcgccac tagacccgaa
       61 ggtctcgttc gacttgcatg tgttaggcat gccgccagcg ttcgttctga gccagaatca
      121 aactctcaag ttgatgtacg acaagcccgg gcggaatagg cccgggcaag ccggcatctc
      181 tgggagccgt tcctgcacaa aatcacaatt ggtgtattgc gttttgagac atacgaacgg
      241 cttggctttt tccgacctac ccgaacccag aggttccggg agaccgggcc gccgcccaca
      301 tgtcccttca tctaaaccga caatgtcaaa gagccgacgc gtatcctccc ccggttcgtt
      361 tggttccggg catcggttgt accgactaag aagagacgcg aaacggtaac ggacctcgtt
      421 gaggccgccg ccgttgctgg aggcgccata tatgggtggc tctccagctc gtcaacggcc
      481 tttttgcaat ttcttgcagg ccgctttcgg ttcgcatgaa cctcgcgaat tgaggatggt
      541 cgatccgaag accggaccgc cccgccgcct aaaacctaga tagtccttcg aaagccgcgt
      601 tcaaggcccg taagagcctg gaacataatc agatttcgaa gaacgaaagc gccaagcgat
      661 tcgctgaagc gggagataga cgctttgcca gtccgatcaa gaggtccgtt gcgaattgcg
      721 acgaatcgag ttcaggcggt ctggatgtac tcgcgcatcg ccgccgcttc ctcttcgatt
      781 cgctcgatgc ggtgcttcac caggtcgccg atcgacacga tccccaccag acggccaccc
      841 gagaccaccg gcaaatggcg aatccggcgc tgagtaatca tcgccaacgc cgccagcacg
      901 ggcgtggccg gctcgacggt caccgcaggc gcgctcatca cccggctcac aggccagtcg
      961 agcgcctcgg ccccgtgctc ccgcaggcaa tagatgacgt cgcgttccga catgatcccg
     1021 gcgacctcgt caccttccag gaccgggagt gctccgatcc tgcgctcgcc gagcacccgg
     1081 accgcatcgg caaccgtcgc gttgccggtg atggtcgcca cgtcggtccc cttgtctcca
     1141 aggatcgtct ggacgttcat ctgcgcctcc ggaattcgtg ggactcgacg ccttgatagt
     1201 gcgactccgc gcggccaaag ggaaggccat gcctagcccg gatccgctcc caagcccgcg
     1261 cctgtcgctg ctccggcgat tccggcgcat catgagatgg atggcgctgt tctcgatcgt
     1321 cgtcgcgtcg attgcggtgc ttctcgttgc ccgtggagac acgcacgtac ctgtgcacat
     1381 gctgatcgcg accgctctcg gcgccggcct gtccgtactc cttgccggag cactgatgag
     1441 cctcgtgttc ctgagcaacg acagcgggca cgacgagaaa gcgaaccgat tcgaagagga
     1501 agaccgaccg tgaccgtcac caaccgcgat cccgtgctgc gcgtcgtccc cggccccagc
     1561 gacatcaacg ccaatggcca catcttcggt ggctgggtac tttctcaaat ggacatcgcc
     1621 gggggcatta ccgcctcgcg ccgcgcgaac ggcccggttg caaccgtcgc gatcgaacgg
     1681 atggagttca tcactcccat ccacctgcgc gacctgatct cggtttatgc cgaggtggag
     1741 cgggtcggcc gtacgtcaat gggcgtgcgg atcgaggtca tcgccagccg cgaccgcggg
     1801 gcgaccgaac tcaaggtgac cgaaggtctg ttcaccttcg tagcgctcga cgagaaccat
     1861 cgccctcggg cggtcgagcc tcagccctcc gcgtccagcg agtagccagc cgaccgcacg
     1921 gtccggatga tgtcccgctt tccgccgtcg ttaagcgcct tgcgcaggcg tcgcacgtga
     1981 acgtcgaccg ttctcaggtc gatgtcgggg tcgtgagacc agactgaatc gagcagccgc
     2041 tcgcgcgaga acacgcgccc ggggttctcg aggaagtgct tcagcaggcg gaactcggtc
     2101 gggccgagcg aaatcggatg cccgtcgcgg cgcacgcgat gagcgcccac gtccatctcc
     2161 acatcgtcat aggtcagctg ctccgccgcg agcgccggcc gcacgcgccg gagaaccgcg
     2221 ccgacgcgcg caaccagttc gcgcgggctg aacggcttgg tgacgtaatc gtcggcgccg
     2281 gtctcgagac cgcgaatccg atccccctcc tccccgcgcg cggtgagcat gatgatcgga
     2341 acattggcgg tttcggacct gcggcggagg cgccggcaaa cttcgaggcc aaggattccc
     2401 tcgatcatcc agtccaatat gatgacgtcc ggcgcgtcct cggacgccag gagcagcgct
     2461 tcctcgccgt cgccggtgcg gcgaacgtcg aaatcctcgc gctcgaaatg ccagatcaga
     2521 agctcagcga gagctcgatc gtcctcgacc aggagtagct ttttgcgggt catgtcgttt
     2581 tccgccgccg tttcttggtg ggttcaacca tgtgatcgcc ggttgccgca taatagacca
     2641 tctctgcgat gttcgtcgcg tgatcgccga cccgctcgag gttcttggcc acg
//
LOCUS       DATAOZ010000275         3474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7219973, whole genome shotgun sequence.
ACCESSION   DATAOZ010000275 DATAOZ010000000
VERSION     DATAOZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7219973"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            621..1610
                     /locus_tag="VHR64_07270"
     CDS             621..1610
                     /locus_tag="VHR64_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3302667.1"
                     /translation="MALFLLAPLPSSWRFGLLLGALLLAIAAAIAAVLLLRSWRELNA
                     AQQLLEMATGNLGEAYTSLVDANQSLRETAIARDDALHRLRTAIRDRDAFLAAISHDL
                     KTPLTVIKGNAELLTGQFKDGVTPDRARVVKGLERITANTGRLTTLVEQLLWLAHLEM
                     DQPVDLNRTCVDLVALTRRVLHEYEATTGLHRLILTPNVAKLEGWWDEGRIGSVLSNL
                     ISNAIKYSPEGGAIDIVIDGDEERQVAILTVRDGGLGIPAVDLPYVFDRFYRAGNVGE
                     LIVGTGMGLAGVRYAVEAHGGRVNVESAEGVGSSFIVELPLSCPSPAQGAPHA"
     gene            complement(1629..2846)
                     /locus_tag="VHR64_07275"
     CDS             complement(1629..2846)
                     /locus_tag="VHR64_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX3302668.1"
                     /translation="MNSAVRWYIGLYSVATAALLLLVLTSSEWSATRGQWIFAALLVV
                     AIAVAQQFPVHLTSKTKVYVDTALITAAALALPPVLAVATVGLPAAIHELRQRISLEQ
                     GVFNVAQTASYVLAGSWIVHALSGTDQMPELSGFRNCAAVAIGLVVMHMINTAAVATV
                     VALQLGRKPLAVWREGLWVDMPEHLVLVTNGVLFAAVGASYPWLLPVFAGPLILVYLS
                     LVRGANLRQVSQATIEAIADLTDLLSGESPGHARRVADLTHRLALELGVTPDEADIAA
                     RAAQLHEVGILRSDPDQPRAIVGRPIHVNSSHAELLDRLRIADSIRHQQERWDGSGAP
                     DGQVREEIPLAARLMAVADAFDQLTSPNDSNTGLSRSVATGMMEMGSGREWDPTVVEA
                     LTRVLSDESPHSV"
     gene            complement(2917..>3474)
                     /locus_tag="VHR64_07280"
     CDS             complement(2917..>3474)
                     /locus_tag="VHR64_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302669.1"
                     /translation="WIFPSAPAGRFALRPESATSNSLSPNLAEVPTMSRSSRLVPILL
                     AALAVLVVAAPVDAGRRWCQKDPIFSIAGTSVPLYVSVYEDQQSHVTGPIAVTLSVPV
                     GVSVTVTYVDGGFNAYGETIKVVTSSRLKVTSKGVQTQFQVSVPADISMPVLVTVAPV
                     SGRSTSITGKTNTVIAVNATITPAT"
BASE COUNT          683 a         1064 c         1088 g          639 t
ORIGIN      
        1 gcctttccag agagttggaa ttattcgaaa catcgaactg tccccttccg ccgcggcact
       61 gaagtcccgc ggccgattcc gggtcacccg ccggccggtc cgattgccgg tcacgtccga
      121 gttacccaac ctcaggttag ggacggggac agctcagaac atcacgaaga acatgtacag
      181 ggacatgcaa ttgcaggcat tgaggtgaat gtccgcgata cgccgcaagg aggcggtaac
      241 gctcgtaccc cgacctcctt agatgccgtt gcgtggaatc gaaggaccca cagtgatgat
      301 cctgagcgca aatggaatcg cgttatccgc acatcccaat gcatattggg tggccttgcg
      361 tcataccttt gggaaaggcg gatgggtggc caacgtagct ttacctcgag aaacacctta
      421 cgatatgcac cgtccccaat acgttggcca tcaggtcagt ttgccgccaa cgtcttcccg
      481 cgagacgagc cgtgctatta ccgccagatt cgaccgcgtc gcatcaatct gactccgact
      541 ccccgccgct acccacctgg gtcgagcggt gcaaactccc ggcgctcgtt ggggtcgcca
      601 ttctcattgt cgtgctcgct atggcgttgt tcttgcttgc cccgcttccg tcgagctggc
      661 gatttgggct cctgcttggc gccctgctcc tggcgatcgc cgccgctatc gctgccgttc
      721 tccttctgcg atcctggcgc gagctgaacg cggcccaaca actgctcgaa atggcgactg
      781 ggaacctcgg cgaagcgtac acgtcgctcg tcgatgccaa tcagtccttg cgcgagactg
      841 cgatagcccg cgacgacgct cttcaccgac tccggactgc catcagggat cgggatgcat
      901 tcctggcggc gatctcgcat gacctgaaga cgccattgac cgtaatcaag ggcaatgcgg
      961 agctgctgac gggccagttc aaggatgggg tcaccccgga ccgggcccgc gtcgtaaagg
     1021 ggctggagcg gatcacagcg aacaccggtc ggttgacgac gctggtcgag cagttgcttt
     1081 ggctcgctca cttggaaatg gatcagccgg tcgacctcaa tcgaacgtgt gtggatttgg
     1141 ttgccctgac ccgacgcgtg ttgcatgaat atgaggcaac gacggggttg caccgcttga
     1201 tcctgacgcc gaacgtggcc aagctggagg gatggtggga cgaagggcga attggaagcg
     1261 tcctgagtaa cctgatctcc aatgcgatca agtacagccc cgagggcggc gcgatcgaca
     1321 tcgtgatcga cggcgacgag gagcgccagg tcgcgattct caccgtgcgc gacggcggtc
     1381 tcggcatccc tgccgtcgac ttgccatatg ttttcgaccg cttctaccgg gccggaaacg
     1441 tgggcgagtt gatcgtcgga accggaatgg gattggccgg agttcgctac gcagtcgagg
     1501 cgcacggggg acgggtgaac gtcgagagtg ccgaaggagt cggcagctcc ttcatcgtcg
     1561 agttgccgct ttcgtgcccc agtccggccc agggtgcccc gcacgcgtga tcaattccga
     1621 gcgtgggttc agaccgagtg gggcgactcg tcactgagaa ccctggtcaa cgcttcgacc
     1681 acggttggat cccactcccg cccggagccc atttccatca ttccggtcgc gaccgaacgg
     1741 ctgagaccgg tgttggaatc gttcggagac gtcaactgat cgaacgcgtc cgcgaccgcc
     1801 atcagccggg cggcgagcgg gatttcctcg cgcacttgcc catccggcgc gccgctccca
     1861 tcccagcgct cctgctgatg gcgaatcgag tcggcaatgc gcaggcggtc cagcagctca
     1921 gcgtgggagc tgttgacgtg aattggccgg ccaacgattg cgcgcggttg gtccggatcc
     1981 gagcgaagta tcccgacttc atggagctgt gccgcccgcg ccgcgatatc cgcttcgtca
     2041 ggcgtaacac cgagctcgag tgcaagccga tgggtgagat ccgccacgcg gcgcgcatgg
     2101 cccgggcttt ccccggatag cagatcggtg aggtcggcaa tggcttcaat tgtggcttgc
     2161 gaaacctgac gaaggtttgc accgcggaca agagagagat acaccaatat caggggtccg
     2221 gcaaaaacgg gcaagagcca cggataggac gcgcccaccg cggcgaacag gactccgttc
     2281 gtgacaagca cgagatgctc gggcatgtcc acccaaagac cttcgcgcca gacagcaagg
     2341 ggcttcctgc ccaattgcag ggcaacgacg gtcgccacag cggcagtgtt gatcatgtgc
     2401 atgacaacca ggccgatcgc gactgccgcg cagttgcgga atcccgacaa ctcaggcatt
     2461 tggtccgtgc cagacagcgc atgtacgatc cagctccctg ccaggacata cgaggcagtc
     2521 tgcgcgacat tgaacacgcc ctgctccagc gagatccgct gccggagctc gtgaatggcc
     2581 gctgggagcc cgacggtcgc caccgccaga accggcggca acgcgagagc cgcggcggtg
     2641 atgagcgcag tgtcgacata caccttcgtc ttactggtca ggtggactgg gaattgctgc
     2701 gccacagcga ttgcgacgac cagcagtgcg gcgaagatcc actgcccccg tgtcgcgctc
     2761 cattcgctcg aagtcagtac cagcagaagc agcgcagccg tcgccacgct atagaggccg
     2821 atgtaccagc gaactgccga attcaattga ttaccgtcgt tcatagcacc ccaaggactt
     2881 gtcggggctc atcgtcgagc gctcccgatc cagtgctcaa gtggcaggag tgattgtcgc
     2941 gttaactgca ataacggtgt tggttttacc ggtgatggat gtcgagcgtc cggaaacggg
     3001 agcgaccgtt acgagcaccg gcatgctgat atcggcgggg actgagacct ggaactgggt
     3061 ttggaccccc ttgctcgtga ctttcaaccg cgaactggtg acgactttga tggtctcgcc
     3121 gtaagcgttg aagccgccgt ccacgtaggt gacggtgacc gagacgccca ccggcacgct
     3181 cagagtcaca gcgatcgggc cagtgacgtg cgattgttga tcctcgtaga cggatacgta
     3241 gagcggcacc gacgtgccag cgatgctgaa gatcgggtcc ttctggcacc accgtcgtcc
     3301 cgcatcgacg ggtgcggcca ccaccaggac ggcaagcgcg gcgagcagga tgggcacgag
     3361 acggctacta cgtgacatgg tcggaacctc cgccagattg ggcgacaagg agttgctcgt
     3421 cgcgctctcc ggccgaaggg cgaatcgccc ggcgggggct gacgggaaaa tcca
//
LOCUS       DATAOZ010000276         6792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_378429, whole genome shotgun sequence.
ACCESSION   DATAOZ010000276 DATAOZ010000000
VERSION     DATAOZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378429"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1026
                     /locus_tag="VHR64_07285"
     CDS             196..1026
                     /locus_tag="VHR64_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3302670.1"
                     /translation="MLDSSTEPEHTGQVNLYGTQYGNFATDLYAEIRRAAVGEDIGQN
                     GWNTAAEQDQFIQWLGLGPSDYLLDVACGAGGPALRLTRLTGCRVTGIDIHEQGIAQA
                     RDRAALDGLGDWATFEVVDASGSLPFGDATFDVVTCIDAINHFPDRLAVLREWRRVLK
                     PGGRLLIADPITVTGPLTNQEIAIRSSIAFFLFVPPGFDEQVITDAGFALNLREDCTP
                     NTATVAGRWRDAREARAGALRAIEGDETFEGQQRFLDVTARLAEERRLSRFVFVATVS
                     "
     gene            complement(1165..5613)
                     /gene="gltB"
                     /locus_tag="VHR64_07290"
     CDS             complement(1165..5613)
                     /gene="gltB"
                     /locus_tag="VHR64_07290"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX3302671.1"
                     /translation="MPTTADQRYAPLYDAAFEHDACGVGLVVDTHGRPSRDIVTRALA
                     GLVNLTHRGGVGADERTGDGAGLLTQIPVALLAPDLTWRELGVAMTFLPRDPELAEQA
                     KSLLAEAMTDRGLIVRGWRDVPVDPSVLGRIAAETQPAIAQLLVERPAGRTEDEFERD
                     LMLARKAAERTTTAAGIDDFFVVSCSCRTIIYKGFCLPEDLAAFYLDLQDPEFTSAIA
                     LFHQRYSTNTLPTWAMAQPFRYIAHNGEINTIQGNRLWMQARKPLLEYPGVASADELE
                     PLVSMTGSDSLSLDNVVELLYHGGRSLPHAMMMLVPEPWERMADMEPTRRAFYDYHAG
                     LNEQWDGPAALAFSDGILAGATLDRNGLRPLRYAITSDGLLIAGSEAGTVAVDQSTVI
                     EKGRLGPGQMIVVDTRRGVVLRNPEVKAEIAGRQPYAEWLNRQRVNVDRDTSTPGRTP
                     VDNLTAVQASFGYSAEDARLIIQPMAAEAKEPTWSMGDDAPLAVLSERPRPLSAYFRQ
                     RFAQVTNPPIDSLRERSVLSLDTFIGPRGNLLVETEEKAELIRLQSVVITESQLKRIS
                     KLNSDHLRTAKISTLFALNQTDLPTALDVLLASVREQVLSGATVLVLSDRGVDQEHVA
                     IPMLLAVGAIHNDLIRAGMRMAVDLVCETGEVWDVHHLACLIGYSAAAVHPYLALQAA
                     GSLAGTRGHELLSVDELQRNYITALEKGLLKITSRMGISTVMGYLGAQIFETIGLGPE
                     LVGFAFAGTPARLGGIGLAEIEADIRSRHEAAWSDPAAKLSDPGFVRFRKDGEHHGYS
                     PTVAKALQAAATSGNLIDFKAYHDMVREHPPTAVRDLIEIKPLGEAIPLDEVEPAEEI
                     VKRFVVTAMSLGALSPEAFQTLAVAMNRIGARSNSGEGGEDPAWYEPAGDDVGHSKVK
                     QVASGRFGVTARYLAMAEELEIKMAQGSKPGEGGQLPGHKVTDFIARMRHAVPGLPLI
                     SPPPHHDIYSIEDLAQLIYDLRQVNPSAKVGVKLVSVAGVGTIAAGVAKARADYILIS
                     GHSGGTGASPLASIKYAGSPWELGLAEAQQVLVMNGLRSRVRLRTDGGIKTPEDVVIA
                     TLLGAEEYGFGTSVLISIGCDMARQCHLNSCPTGIATQKEELRKRFAGTPEMVINYMM
                     MLAEGVREVMASLGIRKLDDIVGRTDLLTQRSMDGRARMLDLGALFAEPAPENQRRKT
                     IDLEPQSETLDQRILATVGAAIEVGGSVDISDRIETKDRTAGARIAGRIALTHGIAAL
                     KPGTVMARFTGSAGQSFGAFSVGGMRLVLEGEANDYVGKGMAGGEIAILPPRTAPFTT
                     PQVIAGNTILYGATGGSLFVAGKAGERFAVRNSGASAVVEGVGDHGCEYMTGGTVVVL
                     GPTGRNFGAGMTNGLAFVVDELEQFWGRANEDVVLERASINDPELAEMRALIEQHIAL
                     TQSAHAKRLLATWSETVAQTWKVIPAARIELQRQQQAAETEATAAD"
     gene            5974..>6792
                     /locus_tag="VHR64_07295"
     CDS             5974..>6792
                     /locus_tag="VHR64_07295"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX3302672.1"
                     /translation="MLRQNAARIVLGIETWTEGTKIRESERSWTDRARREQVLGRVAE
                     ADVRLIDLQFSDIVGGAKALTIPIELLAVVLEQGYRFDGAALTGGHRKVELDLFLMPD
                     PETLAIFPFEADGQRRARLCCSVVRRDGQPFAGDPRSVLERNLAQARELGYEYRVAVE
                     MEYYLLPGDGTLPDRESGAGYFSVADDQIATTRDAVLTSLQGMGITVGGSHHETGPGQ
                     EELDLPDVGALRMADQLITVRQVIRSVARRHGLRATFMPKPMEEAPGSGMHVFQS"
BASE COUNT         1087 a         2099 c         2428 g         1178 t
ORIGIN      
        1 tcaaaaatct gccgccgggc ttgcggccgg aggacaatga actcggtgcc cgccagtcgc
       61 tggatcaact ggctcgccat ctcgaatgag ccggccgaac cgtgggccgg gcaagattcc
      121 cgcgggcgta tgatcggatc acacaacagc tgacgcgttt ccgtcattcc ttcgaatcgg
      181 cgtaaggagc ggtccatgct tgattcgagc acggagcctg aacacaccgg gcaggtcaat
      241 ctctatggca cccagtacgg gaacttcgcg accgaccttt acgcagaaat tcggagagcg
      301 gcagtcggcg aggatatcgg gcaaaacggc tggaacacag ccgcggagca ggaccagttc
      361 atccagtggc tcggtctcgg tccgtcggat tacctgctcg atgtcgcgtg tggtgctggt
      421 gggccggccc tgcggctgac caggctgacc gggtgtcgcg ttaccgggat cgatattcac
      481 gaacagggca ttgcgcaggc acgcgaccgc gccgcgctgg atgggctcgg cgactgggca
      541 acgttcgaag tcgtcgatgc ctccgggagt ttgccgtttg gcgacgccac cttcgacgtc
      601 gtgacctgta tcgacgcgat caaccacttc ccggatcgtc tggccgttct gcgggagtgg
      661 cgacgggtcc tgaagccggg aggtcgcctt ctcatcgccg acccgattac cgtcacgggt
      721 ccgctcacga accaggagat cgcgatccgc agctcgatcg ccttcttcct cttcgtgccg
      781 cccggcttcg acgagcaggt gataacggac gctgggtttg cactgaacct tcgggaggac
      841 tgcaccccca acaccgcgac cgttgccgga agatggcgcg atgcccggga agctcgagct
      901 ggggcgctgc gcgctatcga aggagacgag accttcgagg gccagcagcg cttcctcgac
      961 gtcaccgccc gcctcgccga agagcgccgc ctctcccgct tcgtcttcgt ggcgaccgtg
     1021 agctagccgg tactgagtcg cgatggtccg ctccgccggg gacttgagtc cccggctcca
     1081 aaccgtcagt cggttgaaac cggctgacgg tattccggca gacgccgttg ctggacgttt
     1141 tggtgagacc gtactgagcc tttctcagtc ggcggcggtg gcttcggtct ccgcggcttg
     1201 ttgttggcgt tggagctcga ttctggcggc ggggatgacc ttccaggttt gggccacggt
     1261 ttccgaccag gtggcgagta ggcgcttggc gtgggctgat tgggtgagcg cgatgtgttg
     1321 ctcgatgagg gcgcgcattt cggcgagctc ggggtcgttg atgctggctc gctcgaggac
     1381 aacgtcctcg ttggcgcggc cccagaactg ctccagttcg tcgacgacga aagcgaggcc
     1441 gttggtcatg ccggcgccga agttgcggcc ggtcgggccg agcacgacga cggtgccgcc
     1501 ggtcatgtac tcgcagccgt gatccccgac accctcgacc accgccgagg cgccactgtt
     1561 gcggaccgcg aaccgctcgc cggccttccc ggccacgaag agcgagccgc cggtcgcgcc
     1621 atagaggatc gtgttgccgg cgatgacttg cggcgtcgtg aacggggcgg tccgcggcgg
     1681 caggatcgcg atctcgccgc cggccatgcc cttgccgacg tagtcgttcg cctcgccctc
     1741 gagcaccagt cgcatgccgc cgaccgagaa cgcgccgaat gattggccgg cgcttccggt
     1801 gaatcgagcc attaccgtgc cgggtttgag cgcagcgatt ccgtgagtaa gagcgatccg
     1861 gccagcgatt cgcgcgccgg cggtgcggtc ctttgtctcg atccgatccg agatgtccac
     1921 tgatccgccc acctcgatgg cagcaccgac cgtcgccagg atgcgctgat cgagcgtctc
     1981 tgactgcggc tccaggtcga tggtcttgcg gcgctgattc tccggtgcgg gctcggcgaa
     2041 gagggcaccc aggtccagca tcctggcccg accatccatc gagcgctgcg tcaggaggtc
     2101 ggtgcggccg acaatgtcgt cgagcttgcg gatgccgagc gaggccatca cctctcggac
     2161 gccttcggcc agcatcatca tgtagttgat gaccatctcg ggcgtgccgg cgaagcgctt
     2221 gcgcagctcc tccttttggg ttgcgatgcc ggtcgggcag ctgttgaggt ggcattggcg
     2281 agccatgtcg cagccgatcg agatcaggac cgacgtgccg aagccgtact cctcggcccc
     2341 gagcaaggtc gcgatgacaa cgtcctcggg cgtcttgatt ccgccgtcgg tgcgcagccg
     2401 gacgcgcgag cggagaccgt tcatgaccag cacttgctgc gcctcggcca agccaagctc
     2461 ccacgggctg ccggcgtact tgatcgaggc gagtggggat gcgccggtgc cgccggagtg
     2521 gccggagatc aggatgtaat cggcccgggc cttggcgact ccggccgcga tcgtcccgac
     2581 cccggcgacg gagaccagct tgacgccgac cttcgcgctt gggttgacct ggcggaggtc
     2641 gtaaatgagc tgggcgaggt cctcgatcga gtagatgtcg tggtgcggcg gcggcgagat
     2701 caggggcaga cccgggaccg cgtggcgcat gcgggcgatg aagtcggtca ccttgtggcc
     2761 ggggagctgg ccgccttcgc cgggcttcga gccctgggcc atcttgatct cgagctcctc
     2821 ggccattgcg aggtagcgag cggtcacgcc gaagcggccg gaggcgacct gcttgacctt
     2881 gctgtggccg acatcgtcgc cggcaggctc gtaccaggcc gggtcctcgc cgccctcgcc
     2941 ggagttggac cgagcaccga tccggttcat ggcgacggcg agggtctgaa aggcctcggg
     3001 cgagagcgcg ccgagcgaca tcgcggtgac cacgaagcgc ttgacgatct cttcggcagg
     3061 ctcgacctca tcgagcggga tcgcttcgcc gagcggcttg atctcgatca ggtcgcggac
     3121 cgcagtcggc gggtgctccc tgaccatgtc gtggtaggcc ttgaagtcaa tcagattgcc
     3181 ggaggtcgcg gcggcctgga gcgctttggc gacggtcggg ctatagccgt gatgctcgcc
     3241 gtccttgcgg aagcggacga agcccggatc ggagagcttg gcggccggat cggaccaggc
     3301 ggcctcgtgg cggctccgga tgtcggcttc gatctcggcc aggccgatcc cgccgagtcg
     3361 ggccggggtg ccggcaaagg cgaacccgac cagctccggg ccgaggccga tcgtctcgaa
     3421 gatctgggcg ccgaggtagc ccatcacggt cgagatgccc atccgcgacg tgatcttgag
     3481 cagccccttc tcgagcgcgg tgatgtagtt gcgctggagc tcgtcgaccg agagcagctc
     3541 atggccgcgc gttccggcca gggatccggc ggcttgcagg gcgaggtatg ggtggacggc
     3601 tgccgcgctg tagccgatca ggcaggcgag gtggtggacg tcccagacct cgccggtctc
     3661 gcagacgagg tcgactgcca tgcgcatgcc cgcccggatc aggtcgttgt ggatcgcgcc
     3721 gactgcgagg agcatcggga tggccacgtg ctcctgatct acgccgcgat ccgacagcac
     3781 cagcacggtc gcgccggaca acacctgctc ccggaccgaa gccagcaaca catcgagagc
     3841 ggtcggcagg tcggtctggt tcagggcgaa cagcgtcgag attttggccg tgcgcagatg
     3901 gtcggagttg agcttgctga tacgtttcag ctgcgactcg gtgatgacga cgctctggag
     3961 gcggatcagc tcggccttct cttcagtttc gaccagaaga ttgccgcgtg gaccgatgaa
     4021 ggtgtcgagc gagaggacag agcgctcgcg cagggagtcg atcggcgggt tggtgacctg
     4081 ggcgaagcgc tgccggaaat aagccgaaag cgggcgcggg cgctcggaga ggacggccag
     4141 cggcgcgtcg tcgcccatcg accaggtcgg ctccttcgcc tcggcggcca tcggctggat
     4201 gatcaggcgg gcgtcctcgg ccgagtagcc gaatgaagcc tggacggcgg tcagattgtc
     4261 gaccggtgtg cggcccgggg tcgacgtgtc gcggtcgaca ttgacccgct ggcggttgag
     4321 ccactcggcg tagggctgcc ggccggcgat ctcggcctta acctccgggt tgcgcagaac
     4381 cacgccgcgg cgggtgtcga cgacgatcat ctggcccgga ccgagccggc ccttctcgat
     4441 cacggtcgac tggtcgacgg cgacggtgcc ggcctcggaa ccagcgatca ggagaccgtc
     4501 ggaggtgatg gcgtagcgga gcgggcgcag gccattgcgg tcgagggtcg ctccggccag
     4561 tatgccgtcg ctgaaggcga gcgcggcggg tccgtcccac tgctcattga gaccggcgtg
     4621 gtagtcgtag aaggcgcggc gggtcggctc catgtcggcc atccgctccc acggctccgg
     4681 cacgagcatc atcatggcgt gcggcaggga tcggccgccg tggtagagca gctccaccac
     4741 gttgtcgagg ctgagcgagt cggagccggt catgctgacc agcggctcga gctcgtcggc
     4801 ggaggcgacg cccgggtatt cgagaagtgg cttgcgggcc tgcatccaga gccggtttcc
     4861 ctggatcgtg ttgatttcgc cgttgtgggc gatgtagcgg aacggctgag ccatggccca
     4921 ggtcggcagg gtgttggtgc tgtagcgctg gtggaagaga gcgatcgcgc tcgtgaactc
     4981 cggatcctgg agatcgagat aaaaggcggc gaggtcctcc ggcaggcaga agcccttgta
     5041 gatgatggtg cggcaggagc aggagacgac gaagaagtcg tcgatcccgg ccgcggtggt
     5101 tgtccgctca gccgccttgc gggccagcat caggtcgcgc tcgaactcgt cttcagtgcg
     5161 cccggcgggt cgctcgacca gcaattgggc gattgccggc tgggtctcgg cggcgatgcg
     5221 gccaaggacc gacggatcga cgggtacgtc tcgccagccg cgcacgatca agccacgatc
     5281 ggtcattgcc tctgcgagaa gcgacttcgc ctgctctgcc agttccgggt cgcggggcag
     5341 gaaggtcatg gcgacgccga gctcgcgcca tgtcaggtcg ggagcgagca gggcgaccgg
     5401 gatctgggtc aggaggcccg cgccgtcgcc ggtgcgctcg tcggcgccga ctccgccacg
     5461 atgggtgagg ttgaccagac cggccagcgc gcgcgtgacg atgtcacggg atggacgccc
     5521 gtgggtgtcg acgacgagcc cgacgccgca ggcatcgtgc tcgaaggcag cgtcgtaaag
     5581 cggcgcatat cgctggtcgg cggtcgtcgg catggtccat cccccgatgc ggctgagcgg
     5641 ggagatcaga tgcgcgctgc aggggccagc catcggcgcg caccccggct cagggagtgt
     5701 tcggatgctc agccgagacg accagccaca ttgccgccga cgccgaactg accagcaggg
     5761 cgaatcgctg cctcagaagc tctgagtcta ggaattgtgc tcgcgtcggc caagggacgc
     5821 atcgcaccac acggtgaatc gatatttgta ctctgggcac aatgcgttga cgacggcacg
     5881 ttccaaagat gccaaaggtg tccaagatgt ccacgtgtcc atttcacgat caatgcgggc
     5941 atctttggaa cagattgtgc tcggggtata gtcatgctgc ggcaaaacgc ggcaaggatc
     6001 gtgctgggga tcgagacatg gacggaaggc acaaagatca gagaatctga gcggtcgtgg
     6061 acggaccggg cgcggcggga gcaggtcctc ggccgggtgg ctgaggcgga cgtccggctg
     6121 atcgatctgc agttcagcga catcgtcggc ggggccaagg cgctgacgat tccgatcgag
     6181 ctgctggcgg tcgtgctgga gcagggctac cggttcgacg gcgcggctct gaccggcggc
     6241 caccgcaagg tcgagcttga tctcttcctg atgcccgatc ccgagacgct ggcgatcttc
     6301 ccgttcgaag cggatggaca gcggcgggcg cggctctgct gctcggtcgt gcggcgggat
     6361 ggacagccgt ttgccggcga tccccgctcg gtgctcgagc gcaatttggc tcaggcgcgg
     6421 gagctcggtt acgagtaccg ggtcgcggtc gagatggagt actacctgct cccgggcgat
     6481 ggcacgctgc cggatcggga gtcgggcgcg ggctatttca gcgtcgcgga cgaccagatc
     6541 gcgacgaccc gcgacgccgt cctgacctcg ctgcaaggca tggggattac cgtcggcgga
     6601 tcgcaccacg agactggccc gggtcaagag gagctcgacc tgccggatgt cggggcgctg
     6661 cggatggccg accagttgat cacagtccgg caggtgatcc gctctgtcgc ccggcgtcat
     6721 gggctgcgcg cgaccttcat gccgaagccg atggaggagg cgcctggctc cgggatgcat
     6781 gttttccaga gt
//
LOCUS       DATAOZ010000277         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3534046, whole genome shotgun sequence.
ACCESSION   DATAOZ010000277 DATAOZ010000000
VERSION     DATAOZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3534046"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..2421
                     /locus_tag="VHR64_07300"
     CDS             157..2421
                     /locus_tag="VHR64_07300"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX3302673.1"
                     /translation="MIRQLLMPFGLGLLVFTFLIMLRPLQEYAEDLVAKNIAPPIIGW
                     LMVLLVPQALALTIPMALLIGLLVGLGRLSADREFVVMMACGISLKRLLIPVGLVSTL
                     SLVATAYIWFYGYPNANANFLDLMVQVLAERAEGNVRPHVFYREIPNLMLYVRDVPPS
                     GDGWDGVFMSDTRPGQPEAVYMARHGRIAINRTKRTVDIVLEDTIRRTLDANGNYDVA
                     NIAQMTVSVDPASVIPVRSGATKGLPEMTVPELKAAAAARERQGLSSHNEWVAIHQKF
                     AIPFACVVFGVIGLALGATHRRDGALGSFVLGLIVVFAYYIPLYLGPQLVKGDIIPPW
                     LAAWLPNIVLGGLAAALFVWRERVADQPIRLPAAIQRRFSKQAGVRRSRSLGILRILD
                     RYVAGLYVRIMLLSAASLMGVFYISTFIDRADKLFKGTATPRMLVDYFWYVTPQYVYY
                     VIPLAVLLATLIAIAVLTRNSELIVMKACGVSLYRLAAPMFVCAILAAGAIYVLDRTV
                     VGPYFEQAERLRLAMNGQATAPLNPLSYGWVQGRSDDLYFFRMYNPSEHKLTGVTRYE
                     FTRGMTRLVRQTYAEEARFVGAAPDAHGEWRVTKGWSRDFDDSGQVRKDSFTPIVNET
                     RVFEPATYYGLEQPDPKFMRLGDLQQYTARMIASGYDMFPQQVWVARRYAFPFVTLIM
                     TLIAVPFAVTIGRGGAMAGIAVGIALALVYWVSISIFAALGGGGLIAPTLAAWAPNML
                     FGAGALYLLLTVRT"
BASE COUNT          446 a          830 c          852 g          556 t
ORIGIN      
        1 gcacccacgg gaggatatgt tcgccggtca ccaggtcctg gtagaacggc tggtgcatgt
       61 gccacagcag tgcgacgcgc gtcataatcg ggtatccggt tgcggggcgc gactcgccgc
      121 gccgtctgtt ccttcatgaa aattatcgat cggtacgtga tccggcaact gctgatgccc
      181 ttcggcctcg gcctgctcgt gttcacgttc ctcatcatgc ttcgtccact gcaggagtac
      241 gccgaggacc tggtggcgaa gaacatcgcg ccgccaatca tcggctggct gatggtactg
      301 ctcgtcccgc aggcgctggc gctgacgatt ccgatggcgc tgctgatcgg cctgctggtc
      361 gggctcggcc gtctgtccgc cgatcgcgaa ttcgtggtga tgatggcgtg cggcatcagc
      421 ctcaagcggt tgctgattcc tgtcggcctg gtctcgacgc tctcgttggt cgccaccgcg
      481 tacatctggt tctacggcta ccccaacgcg aacgcgaact ttctcgattt gatggtccag
      541 gtgcttgccg agcgtgccga aggtaacgtc cggccgcacg tcttctatcg cgagattccg
      601 aatctgatgc tctacgttcg tgacgttccg ccatcaggcg acggctggga cggtgtgttc
      661 atgagcgaca cgcggcccgg acagcctgaa gcggtctaca tggcgcgtca cgggcgcatc
      721 gccatcaacc gcacgaagcg gacggtcgat atcgtcctcg aagacacgat ccgccggacg
      781 ctggatgcga acgggaatta cgacgtggct aacatcgcgc agatgaccgt cagcgtcgat
      841 cctgcatccg tgattccggt ccggagcggc gcgaccaagg gcttgccgga aatgacggta
      901 ccggagctga aggcagcagc cgccgcgcgc gagcggcaag gcctgtccag ccacaacgag
      961 tgggtggcca tccaccagaa gttcgcgatt ccgttcgcgt gcgtcgtgtt cggcgtgatc
     1021 gggttggcgc tgggagcgac acatcgccgc gacggtgcac ttggaagctt cgttctgggg
     1081 ctgatcgtgg tgttcgctta ctacattccg ctctacctgg ggccgcaact cgtcaagggg
     1141 gacatcatcc cgccatggct cgccgcgtgg ctgcccaaca tcgtcctggg cggtctcgcc
     1201 gctgccctgt tcgtctggcg cgagcgcgtc gcagaccagc cgatccgtct gccggccgca
     1261 atccagcgac ggttctcgaa gcaggccggc gtccggcggt cccggtcgct cggcatcttg
     1321 cggatcctgg atcgatacgt cgccggtttg tacgtccgga tcatgctctt gtcggcagcg
     1381 tccctgatgg gggttttcta tatctcgacg ttcatcgatc gagcagacaa actcttcaaa
     1441 ggcacggcca cgccgcggat gctcgtcgat tacttctggt acgtgacgcc gcagtacgtg
     1501 tactacgtca ttccgcttgc ggttctcctc gccacgctga ttgcgattgc ggtcctgaca
     1561 cggaacagcg aactgatcgt catgaaggca tgcggcgtca gcctgtatcg gctggcggcc
     1621 ccgatgttcg tctgcgccat cctcgcggcc ggggcaatct acgtactgga ccggaccgtc
     1681 gtcggcccct atttcgaaca ggctgagcgg ctgcgcctgg cgatgaatgg tcaggcaacc
     1741 gcacccctca acccgttgag ttatgggtgg gtgcagggcc gcagcgacga cctgtacttc
     1801 ttccggatgt acaacccgtc ggaacacaag ctcacgggag tgaccaggta tgaattcacg
     1861 cggggaatga cgcgtcttgt gcgccagacg tacgccgagg aggcgaggtt cgtgggtgcc
     1921 gccccagacg cccatggcga gtggcgagtc acgaagggat ggagccgtga tttcgacgac
     1981 agtggccagg tccggaaaga cagcttcacg ccaatcgtca acgagactcg cgtcttcgag
     2041 ccggcgactt attacgggct cgagcaaccg gatcccaagt tcatgcggct tggcgatctt
     2101 cagcagtaca ccgcacggat gatcgccagt ggttacgaca tgtttccgca gcaggtgtgg
     2161 gtggcgcggc ggtatgcgtt tcccttcgta acgctgatca tgacgctcat cgcggtgccg
     2221 ttcgccgtga ccatcggtcg cgggggcgcg atggccggca ttgcggtcgg catcgccctc
     2281 gccctcgtgt actgggtgtc gatcagcatc ttcgcggcgc ttggcggtgg cgggttgatt
     2341 gcgcccaccc tcgctgcatg ggcgccgaac atgcttttcg gtgccggtgc actctatctg
     2401 ctgctgaccg tcaggacctg accgacaacc ttcagaagcg actggcaggc accgtgagcc
     2461 agctgttgga attctcagac gggcaaacga cccgcgagct gcggcttctt gtctctaggg
     2521 tttcccgtac cgaatgttat cgagccagac ttgtccgctg gtgccaagcg gagtattcgg
     2581 tggctcgact acccacagga gtgcctgaat gtccgagacg gcgggcagct tcgttgtcgc
     2641 ctcgccccgc gatcgcatgt cgtcgaagaa caccgtgatc tcgc
//
LOCUS       DATAOZ010000278         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2747658, whole genome shotgun sequence.
ACCESSION   DATAOZ010000278 DATAOZ010000000
VERSION     DATAOZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2747658"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /gene="trpE"
                     /locus_tag="VHR64_07305"
     CDS             <1..1286
                     /gene="trpE"
                     /locus_tag="VHR64_07305"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX3302674.1"
                     /translation="VATVELNGDTTSHEVADPLAFLERTLARYQSQSVPGVHLPRFAG
                     GAVGYLSYEAVRAFESRVGLADGPGLGYPDSHFMLVDSLLVFDNVSRTIKVVAHLNLD
                     AHPDVETAYAVAATRIDELVAQLRRPMPALPLGGDPIESSIEDRRRVNTDPLVYRKMV
                     EKAREYIAAGDIFQVVLSQRVDVPTPAHPFTIYRALRTINPSPYMFYLDIGDHCIVGA
                     SPELLVRVEDGVVTNHPIAGTRPRGGDPKQDAELAAELLADEKERAEHIMLVDLGRND
                     VGRVSKPGTVRVPRLMEIERFSHVMHIVSNVEGDISDEYSPLDALRACFPAGTVSGAP
                     KVRAMEIIAELEVDRRGVYSGAVGYVSFSGAMDTCIALRTLVYKDGVASLQAGGGIVL
                     DSTPDGEYAECFHKMRALVRAIELAESIESAEGAR"
     gene            1283..1894
                     /locus_tag="VHR64_07310"
     CDS             1283..1894
                     /locus_tag="VHR64_07310"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX3302675.1"
                     /translation="MILLIDNYDSFTYNLYQFLAELGAELQVVRNDMITVDEIKARRS
                     DFDRIVISPGPCTPAEAGICVPLIQQLAGEIPILGVCLGHQSIGAAFGATVVRAPMVL
                     HGKTSEVYHSGQGVFAGLPSPFTATRYHSLIVERDSLPPALEITAQTADGVIMGLRHR
                     ELPVEGVQFHPESILTPVGKQLLGNFVGAQVASREPAPALIGY"
     gene            1896..>2392
                     /gene="trpD"
                     /locus_tag="VHR64_07315"
     CDS             1896..>2392
                     /gene="trpD"
                     /locus_tag="VHR64_07315"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX3302676.1"
                     /translation="MATAIDVNLAEILNRLARGTSLTRAEAADALRAVMTGSVSEIQT
                     AALLTALRTKGETVDEVAGFAEAMRAHATQVTLAPDERPVVDTCGTGGDGYHTFNVST
                     TAAFVVAGAGVLVAKHGNRAASSKTGSADLLEALVASIVASPERVAESIARTGFGYMH
                     APEYHP"
BASE COUNT          397 a          756 c          801 g          438 t
ORIGIN      
        1 gcgtcgcgac cgtcgagctc aacggcgaca cgacatcgca cgaggtggcc gaccctctcg
       61 cgttcctcga gcgaacgctc gcgcggtacc agtcgcaatc ggttcccggc gtgcatctgc
      121 cgcggtttgc cggcggcgcg gtcggatatc tgtcctacga ggcagtgcgg gcgttcgagt
      181 cgcgggttgg tctggcggac ggtccagggc tcggctatcc cgattcgcac ttcatgctgg
      241 ttgactcgct gcttgttttc gacaacgtca gccgcacgat caaggtcgtc gcgcatctca
      301 acctggacgc gcatcccgat gtcgaaactg cctatgcggt cgcagcgacc cggatcgacg
      361 agctcgtcgc ccaactccgc aggccaatgc cggcacttcc cttgggcggc gatccgattg
      421 aatcgagcat cgaggatcgc cgtcgggtga ataccgatcc cctggtctac cggaagatgg
      481 tcgagaaggc acgggagtac atcgcggccg gcgacatctt ccaggtcgtc ctgtcgcaac
      541 gggtcgatgt cccgactccg gcgcacccat tcacaatcta ccgggccctg cgcacgatca
      601 acccgagccc gtacatgttc tatctggata tcggcgatca ctgcatcgtc ggcgcatccc
      661 cggagcttct ggtccgggtc gaggacggcg tcgtcaccaa tcacccgatc gccggcacgc
      721 gaccgcgtgg cggcgacccg aagcaggatg ccgagttggc ggcggaattg ttggctgacg
      781 agaaggagcg ggcggagcat attatgctcg tcgatctcgg acgcaacgat gtcggccgcg
      841 tctcgaagcc cggcaccgtg cgggtgccgc ggctgatgga gatcgagcgc ttctcgcacg
      901 tgatgcacat cgtcagcaat gtcgaaggcg atatcagcga cgagtacagc ccgctcgacg
      961 cgctccgggc gtgcttcccg gcaggaaccg tctcgggcgc gccgaaggtg cgggcgatgg
     1021 agatcatcgc cgagttggag gtcgatcgcc gcggcgtcta ttcgggcgcg gtcgggtacg
     1081 tcagcttcag cggagcgatg gacacctgca tcgcgctccg gacgctcgtc tacaaggacg
     1141 gcgttgcgtc gctccaggct ggcggcggca tcgttctcga tagcacgccg gacggcgaat
     1201 atgcggagtg cttccacaag atgcgggcgc tggttcgggc gatcgagctg gccgagtcga
     1261 ttgagagcgc ggagggtgcg cgatgatcct gctgatcgac aactacgact catttaccta
     1321 caacctgtat cagttcctcg ccgagcttgg cgccgagctg caggttgtcc ggaacgacat
     1381 gatcacggtc gacgagatca aggcgcggcg atcggacttc gaccggatcg tgatctcccc
     1441 gggaccctgc acgccggccg aggcgggaat ctgcgtgccg ctgatccagc agttggccgg
     1501 tgagattccg atcctcggcg tgtgcctcgg tcatcagtcg atcggcgcgg cctttggcgc
     1561 aactgtggtg cgggcgccga tggtgttgca cggcaagacg tccgaggtct accactccgg
     1621 gcagggtgtc tttgccggac tgcccagtcc cttcacggcg actcgctatc actcgctgat
     1681 cgtcgagcgg gattcgcttc cgccggcgct cgagatcacc gcgcaaacgg cggacggtgt
     1741 gattatgggg ttgcgccacc gtgagctgcc ggtcgagggc gtgcagtttc acccggaatc
     1801 gatcctgacc ccggtcggga agcagctact tggtaacttc gtcggcgctc aggtggcttc
     1861 ccgcgagcca gcgcctgcgt tgatcggata ctgagatggc gactgcaatc gacgtcaatc
     1921 tggcggaaat tctgaatcgt ctggctcggg gcacatcact gactcgtgcg gaggcggccg
     1981 atgcccttcg cgccgtcatg accggatctg tgtccgagat ccagactgcg gcgctcctga
     2041 ccgcattgcg gaccaagggc gagaccgtcg atgaggtggc tggcttcgca gaggcgatgc
     2101 gggcgcatgc cacgcaggtc acgctggctc cagatgagcg gccggttgtc gatacatgcg
     2161 gcaccggcgg cgacggctac cacacgttca acgtctcgac gacggctgca ttcgtcgtgg
     2221 ctggcgccgg cgtgctggtc gcgaagcacg gcaatcgggc ggcatcgagc aagaccggat
     2281 cggccgacct gctcgaagcg ctggtcgcgt cgatcgtcgc gtcgcccgag cgcgtcgccg
     2341 aatcgatcgc gcggaccggg ttcggctaca tgcatgcgcc cgaataccac cc
//
LOCUS       DATAOZ010000279         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5113819, whole genome shotgun sequence.
ACCESSION   DATAOZ010000279 DATAOZ010000000
VERSION     DATAOZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5113819"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VHR64_07320"
     CDS             complement(<1..249)
                     /locus_tag="VHR64_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302677.1"
                     /translation="MDQVIQLIGALLILSGFAGSQFGYLDQRSWTYLIVNAVGSAILA
                     VLGWYERQWGFFLLEFVWALVSLWSMYGKWRGHEPSPAA"
     regulatory      complement(261..330)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01763"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-III (ykkC-III) riboswitch; guanidine
                     riboswitches control genes whose products are involved in
                     modification or pumping out guanidine as a toxic compound
                     from bacteria; this is a third class of guanidine
                     riboswitches based on ykkC-III RNA motif; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF01763"
     gene            417..1334
                     /locus_tag="VHR64_07325"
     CDS             417..1334
                     /locus_tag="VHR64_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX3302678.1"
                     /translation="MEITAPHLHRFAWISIVAALITILLKFSAWWVTDSVGLLSDALE
                     SFVNLVGAIAALIALWYAAREPDQEHAYGHAKAEYFSSGLEGSLILLAAVSIIYTSIE
                     RLINPSEVEKAGLGVAIAAAAAVVNLVAARWIQRAGQHHNSIALEAGARHLMTDVLTT
                     VGVILGIVLIHFTGWSRLDPIIALVVAANIIRVGIDLMKRSMLGLLDTALPPAELTRI
                     EGVLDRFRQADHIEAHALRTRQAGQRRFGSVHILVPGDWTVDHGHQLTEQIERDIREA
                     VPGIMMVTHLESLDDPRSWDDTELPVPGH"
     gene            1395..1466
                     /locus_tag="VHR64_07330"
     tRNA            1395..1466
                     /locus_tag="VHR64_07330"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1427..1429,aa:Ile,seq:cat)
     gene            1508..1900
                     /gene="rsfS"
                     /locus_tag="VHR64_07335"
     CDS             1508..1900
                     /gene="rsfS"
                     /locus_tag="VHR64_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX3302679.1"
                     /translation="MEPQTDQNPVSQTERVAEARALALHVADLLTDTPASNTVVLDIS
                     ERSSFADFFVICSGENERQLRAIANSVREELSDEGRYPRRSEGEFISGWIVLDYGDVV
                     VHVFDVDQRDFYRLEELWSDAVTVLAIQ"
     gene            1922..2113
                     /locus_tag="VHR64_07340"
     CDS             1922..2113
                     /locus_tag="VHR64_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302680.1"
                     /translation="MNDQNKSNSSIAVFLAGVAIGGVGGGIAGWLLGGHVAPLLTSLL
                     NLIDRDSNKRTVNFEVLQQ"
     gene            complement(2118..>2949)
                     /locus_tag="VHR64_07345"
     CDS             complement(2118..>2949)
                     /locus_tag="VHR64_07345"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX3302681.1"
                     /translation="SCLLVGALVMCAVGALDDTRRVRALFKLYAQIGVAVLAWCGGFR
                     ILAIEVPFLGVLSMGVFALPVTVAWIVGITNAINLIDGLDGLAGSLTLVACAILFLHT
                     YFWPRDDPQFTISLLPAALGGAVLGFLIYNWHPARIILGDAGAYFLGLALAVAAIIGG
                     AKIATTLLALGLPILDVAWVAISRAQRGSSVVQADRGHLHHRLLDLGWSQARIVASVA
                     GASLAFGIASLLLPNRELKLAAIIIVGLVLIGTVVIVATVDEDGNRKLGTRLPGARSR
                     "
BASE COUNT          563 a          981 c          912 g          493 t
ORIGIN      
        1 cgcggcggga ctgggctcgt gaccgcgcca cttaccatac atgctccaga gcgagaccaa
       61 tgcccagacg aactcgagca ggaagaagcc ccactgccgc tcgtaccagc ccaggacggc
      121 caaaatagcg gaacccaccg cgttgacgat caggtaggtc caggatcgct ggtcgagata
      181 gccgaactgg gagccggcaa atccgctcaa gatcaggagc gcgccgatca actggataac
      241 ctggtccatg gcaagactcc gctgcgtcgt cttggcagac cgggtacggc gcacgtctcg
      301 tccgaggctg gcccacacat ggaccgccgc ccgagaaggt accagatcgg gcgtgaatgc
      361 tgcccgaatg gcggcacata tcgttgatag cccggccaat ttggtaccgt tcgagcatgg
      421 agatcaccgc accgcacctt catcgcttcg cctggatctc gatcgtcgcc gccctgatca
      481 cgatcctgct caaattctcc gcctggtggg tcaccgattc agtcggtttg ctctcggatg
      541 ccctggagtc gttcgtcaat ctcgtcggcg cgatcgccgc gctcatcgcg ctctggtacg
      601 cagcgcggga gcccgaccag gagcacgcct acggccacgc caaggccgaa tacttcagca
      661 gcgggctgga ggggtcgctg attctgctgg ccgcagtcag catcatctac acctcgatcg
      721 agcgcttgat caatccgtcc gaagtcgaga aggccggtct gggtgtcgcg atcgccgccg
      781 cggccgcagt cgtcaaccta gtcgcggcca ggtggatcca gcgcgccggc cagcaccaca
      841 actcgatcgc gctcgaagcc ggcgcgcgcc atctgatgac cgacgtgctg acgaccgtcg
      901 gcgtgatcct cggcatcgtg ctcatccatt tcaccggttg gtcgcgtctc gacccgatca
      961 tcgcgctcgt cgtggccgcc aacatcatcc gggtcggaat cgatctgatg aagcgctcga
     1021 tgctcggtct gctcgacacc gcccttccgc ccgcggagct gacccggatc gaaggcgtgc
     1081 tcgatcgttt ccggcaggcg gatcacatcg aggcccacgc cctgcggacg cgccaggcgg
     1141 gccagcggcg tttcggctcg gtccacatcc tggtcccggg agactggacc gtcgaccacg
     1201 ggcaccagct cacggagcag atcgagcggg atatccgcga agcggtgccc gggatcatga
     1261 tggtcacgca cctcgagtcg ctcgacgatc cccgctcctg ggacgacacg gagttgccgg
     1321 tacccggcca ttgatcgtgc aagcgcgtcg tgccctttcg atcacgcgtg ctaatatcct
     1381 tgttaccggt ccatgggcct gtagctcagt ggcagagcac ggggctcata accccagggt
     1441 cgcaggttcg aatcctgccg ggcctaccgc agttgcccaa tcacgcatgt cagatattgg
     1501 aggctatttg gagccgcaaa ccgatcaaaa cccagtctcg caaaccgagc gcgtggccga
     1561 ggccagagcg ctcgcgttgc atgttgccga tctcctgacc gacacgccgg catccaatac
     1621 ggtcgtcctg gatatcagcg agcgttcatc cttcgccgac tttttcgtga tatgttccgg
     1681 ggagaacgag cgacaactcc gcgcgatcgc caactcggtt cgcgaggagc tctccgatga
     1741 gggccggtac ccgcgccgga gtgagggcga gttcatctcg ggatggatcg tgctcgacta
     1801 cggcgacgtt gtcgtccacg ttttcgatgt cgaccagcgc gacttctacc ggctggaaga
     1861 gctttggtcg gatgcggtta ccgtactagc gatccagtaa tcatcagcgg agtcaggccg
     1921 gatgaacgat cagaacaagt ccaattcctc aatcgcggtc ttccttgccg gcgtggcgat
     1981 cggcggtgtc ggcggcggaa tcgctggctg gctgctcggc ggtcatgtgg cccctctcct
     2041 gaccagcctg ctcaacctca tcgatcgcga tagcaacaag cggacggtca acttcgaggt
     2101 gcttcagcag tagccgatca gcggctgcgc gcgcccggca atcgcgtgcc aagcttgcgg
     2161 ttgccatctt cgtcgaccgt cgcgacgatc acaaccgtcc cgatcaagac cagcccgacg
     2221 atgatgattg cggccagctt taactcccgg ttcggcaaca gcagcgacgc gatcccgaac
     2281 gcgagcgatg ccccggcgac cgatgccacg atccgcgcct gactccagcc cagatccagc
     2341 aatcgatgat gcaggtggcc acgatcggcc tgcacgaccg acgagccgcg ctgggcccgg
     2401 ctgatcgcaa cccaggcgac gtccaggatc ggcagaccga gcgcgagcag cgtggtcgcg
     2461 atcttggccc cgccgatgat cgccgcgacc gccagcgcaa gaccgaggaa gtaggcgccg
     2521 gcgtcgccga ggatgatcct ggccgggtgc cagttgtaga tcaggaagcc gagcaccgcc
     2581 ccaccgagcg cggccggcag gagcgagatc gtgaactgcg gatcatcgcg cggccagaaa
     2641 taggtgtgca ggaacaggat cgcgcaggcc acgagcgtca gcgatcccgc cagcccgtcg
     2701 agtccgtcga tcaggttgat cgcgttcgta atgccgacaa tccaggccac ggtcacgggc
     2761 agcgcgaaca cgcccatcga gagaacgccc aggaacggca cctcgatcgc gaggattcgg
     2821 aaaccgccgc accacgcgag gacggcgact ccgatttgcg cgtacaactt gaagagcgcg
     2881 cgcacgcggc gcgtatcgtc gagcgctccc accgcgcaca tgaccaaggc gccgaccagc
     2941 aggcaggag
//
LOCUS       DATAOZ010000280         6124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8799809, whole genome shotgun sequence.
ACCESSION   DATAOZ010000280 DATAOZ010000000
VERSION     DATAOZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8799809"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..505
                     /locus_tag="VHR64_07350"
     CDS             26..505
                     /locus_tag="VHR64_07350"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3302682.1"
                     /translation="MTQKIRPFLWFDGNAEEAVNFYLSVFPDARLGEVSRYGEGMPMP
                     AGTVLVANFTLFGQEFGAINAGPEFKFTEAISFMIDCKDQAEVDYYWDKLTADGGEPS
                     QCGWLKDKYGLSWQVVPSALGELMTDPDPAKANRVTQALMQMSKIDVAKLEAARDGA"
     gene            complement(576..1322)
                     /locus_tag="VHR64_07355"
     CDS             complement(576..1322)
                     /locus_tag="VHR64_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302683.1"
                     /translation="MTEVIAFEAGGYRYIKGVFQYSAGVAAEPGYAIVRARLSRPMPL
                     LDGFALVESHLESIGRPTTAFCACELRSPEPFSEQGFVEFNRQYVQTLERWGLYRDGE
                     NPVARTNVCPEFDKPAGPSLHAFSYAIPSETTRGSFIIAGGGEAREGAGDYRDSIVRL
                     GDISVDGLREKVRYVMTAMESRLIALGFGWGDAISTQAYTVHDIGSLVREEIVERGVT
                     GGLTWHYCRPPVVDIEYEMDVRGSAMEIVL"
     gene            complement(1355..2431)
                     /locus_tag="VHR64_07360"
     CDS             complement(1355..2431)
                     /locus_tag="VHR64_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HEX3302684.1"
                     /translation="MSREWTASAVRAIDRDFARSADTHLVPLDLPGVPAVKVYIKDES
                     THLTGSLKHRLARSLFLYGLCNGSIKEGTTIVEASSGSTAVSEAYFARLLGLPFIAVM
                     PAGTSAAKVEQIEFYGGQCHFVDDPTDVYRVAAELADSCGGHYIDQFTNAALATDWRG
                     NNNIAEAIFEQMRQEPFPAPRWIVVGAGTGGTSATIGRFIRYRGYDTQLCVVDPTDSV
                     FYGYFMDRHREALDYAHSSGIEGIGRPRVEPSFIPDVIDRMIQVHNTASFAAMHFLAE
                     VTGRKYGGSTGTNLYGVFLLAAEMAEAGESGSIVTLACDPGDRYLATYYDRNWLTRNG
                     RDIEPHLRQFRAFFARGVWEPVVV"
     gene            complement(2463..2678)
                     /locus_tag="VHR64_07365"
     CDS             complement(2463..2678)
                     /locus_tag="VHR64_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3302685.1"
                     /translation="MDSAPDINPGELEDILKKDHYTPEELSVLLGINKHAIEAAAHSG
                     DLRATLLEHHIISIRREDVVRWIESNR"
     gene            2844..3095
                     /locus_tag="VHR64_07370"
     CDS             2844..3095
                     /locus_tag="VHR64_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302686.1"
                     /translation="MTERRPDPFESDDNTPQPGEGRLPGIDLHVGGPPQTSGDDNLAA
                     YDEENMYGNDVIVEEDIYDDPDLTDEPMEPNTRVVDEPL"
     gene            3124..3354
                     /locus_tag="VHR64_07375"
     CDS             3124..3354
                     /locus_tag="VHR64_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302687.1"
                     /translation="MAFRTPDTVPNERTSKGGQPYKDEREEMAQKESDLRTKAEDLGE
                     SQKNEPAGDRLARMESDAEERFEEIQEDRSNR"
     gene            complement(3426..3647)
                     /locus_tag="VHR64_07380"
     CDS             complement(3426..3647)
                     /locus_tag="VHR64_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3302688.1"
                     /translation="MTTRTVTSHEPIDELLRQDHYTPEELAELLGMDIWSVRRAARIG
                     ELPAFIVDHHVLCIRREDAIRWLANRQVQ"
     gene            3790..5673
                     /locus_tag="VHR64_07385"
     CDS             3790..5673
                     /locus_tag="VHR64_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX3302689.1"
                     /translation="MPIGVSPDRGVSVMAEQPLGDFTATRRIVMLSAIAIVLGGVSAF
                     LALILLRMIAFVTNLFFFGRLSTATVSPADNNLGAFVILVPVIGALIIGMMARFGSER
                     IRGHGIPEAIEAILIGGSRIQPRVAVLKPLSSGISIGSGGPFGAEGPIIMTGGAFGSL
                     VAQFFNLTSAERKTLLVAGAAAGMSATFASPVAAVLLAVELLLFEWKPRSLVPVALAS
                     ATAMILRRYLLGLGPLFPTPIHEAYIGPWGLALCVVAGLLAGGLSALLTAMVYAAEDA
                     FHRLPIHWMWWPAIGGLVVGVGGLIFPEALGVGYDVIETLIRGDATTTLILGVLLVKS
                     TIWAVSLGSGTSGGVLAPLLMRGGALGGLEAMVFPDHGPGFWPIIGMAAILGGTMRAP
                     LTGIVFTLELTHDINLLLPLLCATAVAHGCTVLIMRRSILTEKIDRRGFHVSREYAVD
                     PLEVLFVRDVMRTEIVALPAEMPLDAVLEPQRIRDDRGGFGQGLYPVLDDNERLVGVV
                     TRSDLRALVPNAPGNGHVRTLREIAYEHPLVAFPNESLRRVMERMATTGLTRFPVVLR
                     DQPNKLVGMITLADFLRARQRSLEEERTRERVLTIRFPIRGDDRTLSPNGTNKAVAKS
                     RDR"
     gene            complement(5698..>6124)
                     /locus_tag="VHR64_07390"
     CDS             complement(5698..>6124)
                     /locus_tag="VHR64_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3302690.1"
                     /translation="NRTVTEKPLTDQDFQALAAFRAALRQFVRFSEEAAREAGLTPQQ
                     HQALVAIRGHIGNEPPTVGELAEALQVKHHSAVGLVDRIVQRGFARREPSTVDNRRVH
                     VVITPAGDEILRSLTSAHREEHRQLADVLRRLTEEYESS"
BASE COUNT         1127 a         1965 c         1894 g         1138 t
ORIGIN      
        1 gtgagttgac atcgaggggt ttcagatgac acagaagatc aggccatttc tttggttcga
       61 cggcaacgct gaggaagccg tgaactttta cctgtccgtc ttcccggatg ccaggcttgg
      121 cgaagtgtcg cgctatggcg agggcatgcc gatgcccgcc ggaaccgtgc tggtcgcgaa
      181 cttcaccctc ttcgggcagg agttcggcgc gatcaacgcc ggaccggagt tcaagttcac
      241 cgaggccatc tccttcatga tcgattgcaa ggatcaggcc gaggtcgact actactggga
      301 caagctcacg gccgatggcg gagagccaag ccagtgcggt tggctgaagg acaagtacgg
      361 cctgtcgtgg caggtggtgc cgtcggccct cggcgaactg atgactgatc ccgatccagc
      421 gaaggcaaat cgagtcaccc aggcgctgat gcagatgagc aagatcgatg tcgccaagct
      481 cgaagccgca cgcgacggcg cctaaccatc tcgcgaagac ctaagccacc gcccccggat
      541 tccgatgaat ggccgggggc ggtggtcttt tcgcgctaaa ggacgatctc cattgcggag
      601 ccgcggacgt ccatctcgta ttcaatgtcg accacaggtg ggcggcaata gtgccaggtc
      661 agcccgccgg tcacgccgcg ctcgacgatc tcttcgcgga ccagcgaccc gatgtcgtgg
      721 accgtgtacg cctgggttga gatcgcgtcg ccccatccga acccgagtgc tatcagccgc
      781 gactccatcg ctgtcatgac gtagcggacc ttctcgcgca aaccatcgac ggatatgtcg
      841 ccgagccgaa cgatgctgtc gcgataatcg ccggcgccct ccctggcttc gccaccaccc
      901 gcgatgatga aactcccgcg tgtcgtctcc gaaggtatgg catatgagaa ggcgtgcagc
      961 gagggaccag ccggcttgtc gaactcagga cagacgttgg tccgggccac aggattctcg
     1021 ccgtcccggt agagtcccca ccgctcgagc gtctgcacgt actggcggtt gaactcgaca
     1081 aacccttgtt cgctgaacgg ctccggcgat cgcaactcgc aggcgcagaa cgctgtggtc
     1141 ggtcgcccaa tgctctccag atgcgattcg accagggcga acccatccag gagcggcatc
     1201 ggccggctca ggcgagcccg cacgatcgca tatccgggct cggccgcaac tcctgccgaa
     1261 tactgaaaaa cgcccttgat gtaccggtat ccacccgcct caaacgcaat gacctctgtc
     1321 aatccagact cctcgcccaa tcccgaaatc aaccctatac aaccaccggc tcccagactc
     1381 cgcgcgcaaa gaatgcccgg aattggcgca ggtgcggctc gatatcgcgt ccgttcctcg
     1441 tcagccaatt gcggtcgtaa taggtcgcca ggtaccgatc acctggatcg caggccagtg
     1501 tcacgatcga gccggactcg cccgcctcag ccatctctgc cgcgagtaga aacacgccgt
     1561 acaggttcgt gccggtggat ccgccgtact tgcgaccggt tacttctgcc aggaagtgca
     1621 tcgcggcgaa cgacgcggtg ttgtggacct ggatcatccg gtcgatgaca tccgggatga
     1681 acgagggctc gacccgcggc cggccaatcc cctcaatgcc cgagctgtgc gcgtaatcca
     1741 gcgcctcgcg atgccgatcc atgaagtagc catagaacac ggaatcggtc ggatcgacca
     1801 cacacagctg ggtatcgtac ccgcgatacc ggatgaatcg cccgatcgtg gcagatgttc
     1861 cgccggttcc tgcgccgacc acgatccagc gcggcgccgg gaacggctcc tgccgcatct
     1921 gctcaaagat cgcctcggcg atgttgttgt tgccccgcca gtcggtcgcc aacgcggcgt
     1981 tcgtaaactg gtcgatgtaa tgcccgccac aggagtccgc caactcggcc gccacacgat
     2041 agacatcggt cgggtcgtcg acgaaatggc actgcccgcc atagaactcg atctgctcga
     2101 ctttggctgc cgatgtcccg gccggcatca cggcgatgaa cggcaacccg agcaatcggg
     2161 caaagtacgc ctccgaaact gccgtactcc ctgacgacgc ctcgacgatc gtcgtgccct
     2221 ctttgatcga cccgttgcag aggccataaa ggaagagcga ccgcgcgagg cggtgcttga
     2281 ggctgccggt cagatgcgtc gactcatcct tgatatagac cttgaccgcc ggcacgcccg
     2341 gaagatcgag cggcaccaga tgcgtgtccg ccgagcgggc aaagtcccgg tcgatcgccc
     2401 gaaccgcgct ggccgtccat tccctgctca tcggaccgag agatccctta tctccaccgg
     2461 agtcaacgat tgctctcgat ccaccggacc acgtcctctc gccggatgct gatgatgtga
     2521 tgctcgagga gggttgcccg cagatcgccg gaatgcgcgg ctgcttcgat cgcgtgcttg
     2581 ttgatgccca gcagcaccga aagctcctcg ggcgtgtagt ggtccttctt cagaatgtcc
     2641 tcgagttcgc cggggttgat gtcaggcgcc gaatccatgc tggcctccca tcacgctgaa
     2701 ccgatctctc ctattctggc cgattctcgc ccgtttggta acagggaatg tcggtaggat
     2761 gacgcaccag tcgcatcgcg ctcgccgact agcatagtgg accatgcgcg gccaccggcg
     2821 gcgcgcagaa ggagcgattg accatgaccg agcgcaggcc cgatccattc gagagcgacg
     2881 acaacacacc gcagccgggc gaagggcgac tccccggcat cgacctccac gtcggcggac
     2941 cgccgcaaac atccggcgac gacaatctcg ccgcctacga cgaggaaaac atgtacggca
     3001 acgacgtgat cgtcgaagag gatatctacg acgatcctga cctcaccgat gagccgatgg
     3061 agccgaacac gcgtgttgtg gacgagcccc tgtagcgaag atcaattcag gagggtggat
     3121 gcaatggcat ttcgaacacc cgataccgtg cccaacgagc gcacgtccaa gggcgggcaa
     3181 ccatataagg acgagcgcga ggagatggcg cagaaggaat ccgacttgcg gacgaaagcc
     3241 gaggatctcg gcgagtccca aaagaatgag cccgccggcg atcggctggc gcggatggag
     3301 tccgacgcgg aggaacgctt cgaggaaatc caggaagatc gctcgaatcg ctgaacgtcc
     3361 aatccgatcg tcagaacagg cagaggggcg ctcgggcatg ggcgcccctc attcgatact
     3421 ccgcgctatt gcacctggcg attcgcaagc cagcgaatcg cgtcctcgcg gcgaatgcag
     3481 agcacatgat ggtcgacaat gaacgccggc aactccccga tccgcgccgc ccggcgaacg
     3541 gaccagatat ccatccccag aagctctgcc agctcctccg gcgtgtagtg atcttgccgc
     3601 aaaagctcat cgatcggttc gtggctggtc acggttcgcg tcgtcatcgg attctccctt
     3661 gcccgtagca cgcgactcgc gatcggcggt cgtccactgg caataaagca tcgttttacg
     3721 atactattac gccattgtgg cgatccacgc cactctctcc gttacagacg atccaaggag
     3781 tcctggtaca tgccgatcgg ggttagccct gaccgtggcg tgagcgtgat ggccgagcag
     3841 ccgctcggcg acttcacggc gacgcggcga atcgtgatgt tgtccgccat tgccattgtc
     3901 ttgggcggag tcagcgcatt cctggcgctg atcctgctcc gcatgatcgc gttcgtcacg
     3961 aatctcttct tctttgggcg gctcagcacg gcaaccgtgt cgcccgccga taacaatctc
     4021 ggcgcgttcg tgatccttgt cccggtgatc ggcgcgttga tcatcggcat gatggcccgc
     4081 ttcggctcgg agcgaatccg cggacacggt atcccggagg cgatcgaggc gatcctgatc
     4141 ggcgggagcc gcatccagcc gagggtggca gtgctcaagc cattgtcctc ggggatctcg
     4201 atcggttccg gcggcccgtt cggcgccgag ggtccgatca tcatgaccgg cggcgcgttc
     4261 ggctcgctgg ttgcgcagtt cttcaacctg accagcgccg agcggaaaac gctcctggtg
     4321 gccggagcag cggccggtat gtccgcgacg tttgcttcgc ccgtggccgc tgtcctcctg
     4381 gcggtcgagt tgctgctgtt tgagtggaag ccgcgcagcc tggtaccggt cgccctcgcc
     4441 agcgcaacgg ccatgatctt gcggcgctac ctgctcggct tggggccgct cttcccgacc
     4501 ccgatccatg aggcctacat tggcccgtgg ggactcgccc tctgcgtcgt cgccggtctc
     4561 cttgcgggcg ggctttctgc gttgctcacg gcaatggtct acgccgccga ggatgccttt
     4621 caccgtcttc ccatccattg gatgtggtgg ccggcgatcg gtggactggt ggtcggtgtc
     4681 ggtgggctga tcttccccga agcgctcggc gttgggtacg acgtgatcga gacattgatc
     4741 cgcggcgatg cgaccacaac cctcatcctc ggcgtgctcc tggtcaagtc gaccatctgg
     4801 gcggtctcgc tcggatcggg gacttctggc ggcgtattgg cgccgctcct gatgaggggc
     4861 ggcgcgctcg gcggactcga agcgatggtc ttccccgatc acgggccggg attctggccg
     4921 atcatcggca tggctgcgat cctcggcgga acgatgcgcg ctccgcttac cggcatcgtc
     4981 tttacgctcg agttgaccca cgacatcaac ttgctcctgc cgttgctctg cgcgacggcg
     5041 gtcgcccatg gctgcacggt gctgatcatg cgtcgctcga tcctgaccga gaagatcgat
     5101 cggcgcggct tccacgtcag ccgggagtac gcggtcgacc cgctcgaggt gctctttgtg
     5161 cgcgatgtga tgcggacgga aatcgtcgca ttgccggctg agatgccgct ggacgccgtg
     5221 ctggaaccgc aacggatccg cgacgatcgg ggcggctttg gacaggggct ttacccggtg
     5281 ctcgacgaca atgagcgcct ggtcggagtc gtgacccgat cggacctgcg cgcgctcgtc
     5341 ccgaatgcgc ccggcaatgg ccatgtgcgc acgctcaggg agatcgcata tgagcatccg
     5401 ctggtggcgt ttccaaacga gtcgttgcgc cgggtgatgg agcggatggc gacaacgggt
     5461 ctgacccgat tccccgtcgt cctccgggat caaccgaaca agttggtcgg gatgatcacg
     5521 ctggccgatt tcctgcgcgc gcggcaacgg agcctggagg aggagcgtac ccgcgagcga
     5581 gttctgacaa tccgtttccc gatccggggc gacgaccgga cgctttcacc caatgggacg
     5641 aacaaggcgg ttgcgaagtc tcgcgatcgc tagccaacga aatgcgaatg tgccctgtta
     5701 actggactcg tactcctcgg tcagccgccg caggacgtcg gcaagttgcc gatgttcctc
     5761 ccggtgggct gaggtgagcg aacggaggat ctcgtcaccg gccggagtga tgacgacgtg
     5821 cacgcgccgg ttatccacgg tcgagggctc gcgtcgggca aagccccgct ggacgatgcg
     5881 gtccaccagc ccgaccgccg agtgatgctt gacctgaagt gcctcggcaa gctcaccgac
     5941 cgtgggcggc tcgttgccaa tatgacccct gattgcaacc agggcctgat gctgctgagg
     6001 cgtcaggcct gcctctcgcg ccgcttcctc actgaacctg acgaactgcc ggagcgctgc
     6061 ccgaaacgcg gccagtgcct ggaaatcctg atcggtaagc ggcttctcag ttactgtgcg
     6121 gttc
//
LOCUS       DATAOZ010000281         4456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_642316, whole genome shotgun sequence.
ACCESSION   DATAOZ010000281 DATAOZ010000000
VERSION     DATAOZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642316"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..962
                     /locus_tag="VHR64_07395"
     CDS             111..962
                     /locus_tag="VHR64_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX3302691.1"
                     /translation="MSNDTVTSRWKCLFFGMPCAFSAPILSALSRPDIELVGIVSPQS
                     TPDSGPFRLIRGLRSRATVTLSGQAAHFTTPRYLVRDIGHPRLHSELAALQPDLIIVA
                     CFPRLLPAEIVTTARIAAINVHPSLLPRHRGPDPLFWTFHAGDDQSGATLHLLTDQYD
                     AGDILTQATTSIGTGESLPALERRLAQLGAELIARLIDELPHLPGPTPQDASSASWEP
                     WPRAADRVIDRGWTRERVRRFIAGVASSHGPLTYRDGTQLLSIRSLTDPGMGHEIRLQ
                     DGTLFVA"
     gene            1297..2349
                     /locus_tag="VHR64_07400"
     CDS             1297..2349
                     /locus_tag="VHR64_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3302692.1"
                     /translation="MSLLRVPMGPGISRPVKVLLTALIVAAMLLPLVSAPAPVRAASA
                     YTTDYLNLRAGPSSSNYIKLVMPPGSSVDLVSDLGRSGYYKVSYQGELGYAHSDYLSV
                     GGGSNAVDAGWDNAGSAFTTDYLNLRAGPSTGHDVVTVMPVGATVQLTGAAQSGFSGV
                     VYGGMNGWASTDYLSSDEGGGGTDDPGSGNAGTAYTTSSLNLRTGPSLNRAVIMVMPS
                     GAAVTLTGEARNGFAGVDYNGTTGWASQDYLTTDPAPADDGYYTGDEIVQIIYTAADT
                     YGQSRSAMLAVAQCESLLDPYATNAWSGAAGLFQFLPGTWATTPWAGYDIYDPVANAN
                     AAAWMWSVGRRGEWVC"
     gene            complement(2483..3340)
                     /locus_tag="VHR64_07405"
     CDS             complement(2483..3340)
                     /locus_tag="VHR64_07405"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3302693.1"
                     /translation="MIEQQQDYVLAVTGATLRGRTWPVANPRAVVALAHGINEHVGRY
                     EHVAAALNEAGYSVVGVDHRGHGRSGEDGKRTSNIRRFDTFVDDYIALVDRLRAEQDR
                     PVVALGHSMGGLIVARAALRAQDRMAAVVLSGPALKLPMPLTPRKLRMLLAVSRIAPF
                     LSLPKGDLDGLSRDPEVRTKTLEDALCINAPIRLGIARQLYLLSEETRSRAREIRTPL
                     LVMHGGADPITDPDGSLEFVANASSTDKEFVLWPDDLHEIFNELDQDAVIKKMIDWLN
                     ARFPRLAST"
     gene            3395..>4456
                     /locus_tag="VHR64_07410"
     CDS             3395..>4456
                     /locus_tag="VHR64_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3302694.1"
                     /translation="MHNQHDRTITQIELPARTIIRVAAVFVLLWLLHQLWHILLLGMI
                     ALMLTAALDPPVRRLQARGLSRSWSVAIIMTTVVVGLLGTVLIILSPVITEGQAFLRA
                     LPEQVDRLQGVLKDNPQLYERLRQAAENATGGNSDAVTGNVKQVTFSIISFITDTLVV
                     VTFTIYFLLDGKRIYRWTVRYVPSRYRRKFDRTIPEVSRVVSGYVSGQLLTSLAFGIF
                     SFAVLTAVGVPQPLFLALLAAVGDAIPIVGVTAVTIPTVLLALTISPSAAIIVLLAYL
                     IYQQIENYFIVPRVYQSTLQISSFAVLLAVLIGSALLGIVGALLALPIAAAIPVVEEI
                     WLEDSPLRTNIPPPPPEPME"
BASE COUNT          747 a         1565 c         1331 g          813 t
ORIGIN      
        1 cttgacgcat tggaccattc gcggggtagg ctcactcatc gattcgacct cagtgcggta
       61 gggccgtcaa ctcgacccga tcacgcaatc gtccggcaga tcactacagg atgtcaaacg
      121 acaccgtcac atcgcgttgg aaatgcctct tcttcgggat gccgtgtgcg ttctcggccc
      181 cgatcctgtc cgcgctgtcc cgcccggaca tcgagctggt cggcatcgtc tcaccgcagt
      241 ccactcccga ctcggggccg tttcgtctga ttcgcggtct ccgctcccgg gcgacggtga
      301 cgctctccgg ccaggcagcg cactttacga cgccacgcta tctcgtccgc gatatcggcc
      361 acccgcgact ccattcggag ttggctgcgc tccagcccga tctgatcatc gtcgcctgct
      421 ttccacgcct gctcccggcg gagatcgtca ccaccgcccg gatcgcggcg atcaatgtcc
      481 atccatcgct cttgccccgg caccgcggac ctgatcccct tttctggacc ttccacgcag
      541 gcgacgatca atccggcgcc acgctccact tgctcacgga tcagtacgac gcgggcgaca
      601 tcctcacaca ggcaaccacg tcaatcggaa ccggcgagag cctgccggcg ctcgagcgcc
      661 gcctggccca gctcggcgct gagcttattg ctcgcctgat cgatgagttg ccccacctcc
      721 ccggccctac cccgcaggac gcctcgtccg cgagttggga gccctggccg cgcgcggccg
      781 accgggtgat tgatcggggc tggacacggg aacgagtccg ccgtttcatc gccggcgtgg
      841 catcgtcgca cgggccgctt acctaccggg acggtacgca gctactgtcg attcgcagtc
      901 tcactgaccc aggcatggga catgagatca ggctgcagga cggaacgctc tttgtagcat
      961 agcgttcgtt gccgggaccc attcgggcca gcccgttttg acagcccgcc aatcctgtga
     1021 gataacccgg gtcaatcgct cattgaaacg gcattctgag gcgttttgcg ctgatcgctg
     1081 gctacgatca atgccgatcg aaagcgatgt tcaccccggc ctcatcgagg gatgggccgg
     1141 gcgacccgca ccgcatgcgc gtgtgttggc tggtcgaata tcgaacgcgc gattcgcacg
     1201 cagcgtgaat cgccaccttg cgtcagtcgg atcaggaccg gtcggcccgc ctccgacccc
     1261 cgccctgatc gggatgaccc gaaggagcgg cccatcatgt cgctactgcg tgttcccatg
     1321 ggcccgggga tctcccggcc tgtcaaggta ctgttgaccg cccttatcgt ggcagccatg
     1381 ctcctgccgc tggtctccgc gccggcgccg gtccgcgccg ccagcgccta caccaccgat
     1441 taccttaacc tccgcgccgg acccagcagc agcaactaca tcaagctcgt gatgccgccc
     1501 ggctcctccg tcgatttggt cagcgacctc ggccgctccg gctactacaa ggtcagctac
     1561 cagggcgagc tcgggtacgc ccattccgac tacctgagtg tcggcggcgg gagcaatgcc
     1621 gtcgatgccg gctgggacaa tgccggctcc gccttcacga ccgactacct gaacctgcgg
     1681 gccggtccga gcacgggaca tgacgtcgtg acggtcatgc cagtaggcgc gaccgtccag
     1741 ctcaccggcg cggcgcaaag cggattctcg ggcgtggtct acggcggcat gaatggctgg
     1801 gcctcaaccg attacctgag cagcgacgag ggtggtggcg gtaccgatga tccgggcagc
     1861 ggcaatgctg gcaccgcgta cacgacatcc agcctcaatc tgcgcaccgg gccgagcctg
     1921 aaccgggcag tgatcatggt catgccctcc ggtgcggccg tgacgctgac cggcgaggcg
     1981 aggaacggtt tcgccggcgt cgattacaac ggcacgacgg gctgggcctc gcaggattac
     2041 ctgacgaccg atccggctcc cgccgatgac ggctactaca ccggcgacga gatcgtgcag
     2101 atcatctaca cggctgccga tacctacggc cagtcccgct cggccatgct cgccgtcgcg
     2161 cagtgcgaat cactgcttga cccgtacgcg accaatgcct ggtcgggcgc ggccggtctg
     2221 ttccagttcc tgccgggcac ctgggccacc acgccgtggg ccgggtacga catctatgac
     2281 ccagtcgcca acgccaacgc cgccgcctgg atgtggtccg tcggtcgccg cggcgaatgg
     2341 gtctgctagg gaatcactta ctcgatgtgg cgccgggccc ctgcgcccgg cgatcgctca
     2401 taagctaatg gccaccttgc cggacaaacg attcgcacgc atgacgtagc gcgggttacc
     2461 ccgcgctacg tttgattcgg tcctaagtag aggctaggcg cgggaagcgg gcgttgagcc
     2521 agtcgatcat cttcttgatg accgcatcct gatccagctc gttgaagatc tcgtgcaggt
     2581 cgtcaggcca aagcacgaac tccttgtcgg tcgatgacgc attggccacg aactcgagcg
     2641 acccatccgg gtcggtgatc gggtccgctc cgccatgcat gaccagcaac ggcgtccgga
     2701 tctcccgcgc ccgggatcgc gtctcctccg agagcaggta gagttgccgc gcgatcccga
     2761 gccggatcgg cgcgttgatg cagagcgcgt cctcgagcgt cttcgtgcgc acctccggat
     2821 cacggctcag cccatccagg tcacccttcg gcagggaaag aaacggcgcg atccgggaaa
     2881 cggccagcag catccggagc ttgcggggag tcagcggcat cggcaacttc agcgccggac
     2941 cgctcagcac cacggccgcc atccgatcct gcgcccgcaa tgccgctcgt gccacgatca
     3001 ggccgcccat cgagtgcccg agcgcaacca ccggccgatc ctgctcagcg cgcaatcggt
     3061 cgacgagcgc gatgtagtcg tcgacaaagg tatcgaaccg gcggatgttc gacgtccgct
     3121 tgccgtcctc cccggatcgc ccatgcccgc gatgatcgac cccgacgacc gagtagcccg
     3181 cctcattcag cgccgccgcg acgtgctcat aacgcccgac gtgctcattg atcccgtgcg
     3241 ccagcgccac cacggcccgc ggattcgcca ccggccatgt gcggccgcgc aatgtggctc
     3301 cagtcaccgc gaggacgtag tcctgttgct gctcgatcaa cgcgctggac ctcctcccct
     3361 atcatgtgct cgaacagcag gagttgagct accggtgcat aaccaacacg accgcaccat
     3421 cacccagatc gagcttccgg cgcggaccat catccgggtc gccgccgtgt tcgtgctgct
     3481 ttggctcctg catcagctct ggcacatcct gctgcttggc atgatcgcac tgatgctgac
     3541 cgcggcgctc gatccgccgg tgcggcgctt gcaggcgcgg ggactgagcc gctcatggtc
     3601 ggtcgcgatc atcatgacga cagtcgtcgt cggattgctt gggacggtgc tgatcatcct
     3661 ctcgccggtg atcaccgaag ggcaggcatt cctcagggcg ctgccggagc aggtcgatcg
     3721 cctgcagggc gtcctcaagg acaacccgca gctctacgag cggctgcgtc aggcggccga
     3781 gaatgcaacc ggcggaaatt ccgatgcggt gaccggcaat gtcaagcagg tcacgttcag
     3841 catcatcagc ttcatcaccg acaccctggt cgtcgtcacc ttcacgatct actttctgct
     3901 cgacggcaag cggatctacc gctggacagt ccggtacgtg ccatcgcgct atcgccggaa
     3961 gttcgaccgc actatcccgg aggtcagccg ggtcgtcagc ggctacgtga gcggccaatt
     4021 gctgacctcg ctcgcgttcg gcatcttcag cttcgcggtg ttgacggccg tcggcgtacc
     4081 gcagccgctc ttcctggcgc tactggcggc ggttggcgat gccatcccga tcgtcggcgt
     4141 cactgccgtc acgatcccga ccgtgctgct ggcgctgacc atctcgccct cggcggcgat
     4201 catcgtcctc ctcgcctacc tcatctatca gcaaatcgag aactacttca tcgtgccacg
     4261 ggtctatcaa tcgaccttgc agatctcatc atttgcggtc ctgctggcag tgctgatcgg
     4321 atcggcgctg ctcggcatcg tgggtgcact gctggcactg ccaatcgcgg cggcgatccc
     4381 ggtcgtcgag gagatttggc tcgaggacag cccgctccgt accaacatcc cgccaccccc
     4441 acccgaaccc atggaa
//
LOCUS       DATAOZ010000282         7354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1698623, whole genome shotgun sequence.
ACCESSION   DATAOZ010000282 DATAOZ010000000
VERSION     DATAOZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698623"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VHR64_07415"
     CDS             <1..957
                     /locus_tag="VHR64_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302695.1"
                     /translation="GVSADGGTESPYDRSTAVDETNLSRVVLIREAIDSTGATDMPIW
                     FTHLGWTGSSDSPVSPDDQARFVESAIRRARSEWPWVGLMFNWTFGVEPDVPNSANLA
                     LIVNGNPTPLYDAMSGFAKSSFGASITNGFVPATASACAYSGNWQDQHLAEGIYRTVR
                     DPSSVVTCRFWGTGISVFFRFSPDAGTARYAIDSTDLDSASDRNAGSVILQYRVQDAF
                     EAPVALASGLPEGQHTVTIALDDSGGELVIGGFLVSRERPMIWPIAVLVAAGLVALFL
                     GLRSLAFLAAEHVGLVQPKSDAPAQTPLPILPDWKPAPRFQR"
     gene            954..2291
                     /locus_tag="VHR64_07420"
     CDS             954..2291
                     /locus_tag="VHR64_07420"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX3302696.1"
                     /translation="MTIRRFPPVFGRPDIACILAILISAPVAFGIPTVVKLGLLLLAT
                     GVVVLGGPVAGVSLVCAALSVSAFVFTIGSTEWSPLELALLSCGAATGFVVVLEFARN
                     RRLSVIWKWIGPRDVIFIAVAVLVVGALSILWVADADLRSDSLRSLRRVIIEPLLVIP
                     AMTAVVRARQQAVVARWIAGPAVLVAVLALAQLAAQRSTVDIGGIARPIGTFTHPNNL
                     SFYLERAIWFSPFLAVPFARRGDRWSWAAGGVILLATIATLSRGAAIALAAGGLVMFW
                     EVIRARWRLFAGLATAGVAAIFASRYLAETGDSIDTRTTIWRSSVDMLRDHPVTGVGL
                     DQFLGQYGRRYVRIEGWPERYTSHPHNLFLDFWLSLGLAGLATLWMILEATWSRIRST
                     LGTSGPTVHRAGVALLVAGFAHGLIDNSFFLPYLATMTWIGLTISVLPGRDHG"
     gene            2284..3612
                     /locus_tag="VHR64_07425"
     CDS             2284..3612
                     /locus_tag="VHR64_07425"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX3302697.1"
                     /translation="MADLGRLLVDNIWIWTSLAGVILAAFAFLLSVDGSDWAPILVMA
                     TVPIQRTLLIGTDGHAATWTQAAVAGFFAGAAIRFGAGTLRIRLDGPAILMAAVVSLY
                     GLSIAVADDRGLWAAETYRWAIAGLFLIVARSYFDDRSDSRVALALAAGAVVAFAWAA
                     LQVASEEGPASFVRSGVMRARGGFGEPNPYAAFIWAVTLPLLAFAVAGKGKSRWIRWT
                     SAAVGSLGLAALVMTQSRGGMLGIGAGLCVIGGLLLLRLNPRLRLIGLGSVATIAAAA
                     IVLVAIASPWREIDAATTPGNWADQERTAHWVAAVHMVESHPVTGVGSGEFSANYRAD
                     TGYWRFRISRGHAHNAYLQVAAEVGLPGMLAYACLLAAILGSLIRRLRTTDGSWLALG
                     VAAMTMAMIVHQLVDYLHVLSLGLLFAGLWAAALPSDSRDISSREHNIAA"
     gene            3590..4669
                     /locus_tag="VHR64_07430"
     CDS             3590..4669
                     /locus_tag="VHR64_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX3302698.1"
                     /translation="MNTTLQPEPLIEQTEPVIEQPVTSVVVEAPEPIQRRLFRPHTIA
                     SFAIAIAIIILLVKRLNINLSAVWDNISRANPWYFSAAVILYYLTFIARTYRWRYMLK
                     QAGITRENGYAVPGIPGLFEIMMLSWFANCVVPAKLGDGYRCYLLKRDAGAPASATLG
                     TILAERITDLTVLFVAMSAAGVLTFHGNLPSRVQQTVMIGGILLVFAIVVLVALFFAR
                     DFVQRFVPDRFVRHYHDFHDAVFACLRQPAIPVMVSVGIWIADGFRLFLVARSLGADL
                     TITLTVFVALMSALLTTLPITPAGLGVVEAAVVVVLKLVDVHADLASSIAVLDRVIGY
                     WSLIAFGLLLYLHRMRRDVRVARAA"
     gene            4666..5817
                     /locus_tag="VHR64_07435"
     CDS             4666..5817
                     /locus_tag="VHR64_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3302699.1"
                     /translation="MSQPRLLIDSSAGFNQGAGIGRYSRSVLRAALPSLQSDFDIRLW
                     YASDTRHGARFEREAMDAIPPSLQGHVKRSRWSRRQVDQFARLPIDVPTRLIAGAADV
                     AYSPDFTLPGQFGGAGMITVHDVAFELIPEAYPAGLLQYLRTVVPRNVRRADVVAVVS
                     RTTRIDVVERYNVDSERVVVIPNAADDRFFGASSLGSARRQELGIPDDFLLTVGTIEP
                     RKNYRTLLAAQKIAFLKTGRPLVVVGRSGWGNRDEMQMLNELADEGAVIPLLNADDAD
                     LPGLYCDARAVVYVPLYEGFGLPVLEAMASGAPVITSDIPSVKEVAAGHATIVPPLDV
                     EQMAESMASPVARSAEFRASLRDAARAYSWDASGTILREALTGLAGRAT"
     gene            5817..5987
                     /locus_tag="VHR64_07440"
     CDS             5817..5987
                     /locus_tag="VHR64_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302700.1"
                     /translation="MADEGRYIPLEELDEETRREVIKTRRVIKLVGIVSIGLIVLMAV
                     FTIIAVLLVYGD"
     gene            6007..7011
                     /locus_tag="VHR64_07445"
     CDS             6007..7011
                     /locus_tag="VHR64_07445"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3302701.1"
                     /translation="MEHDQSASIDIAHFRETAHQIEAEVSRVMVAQEDIVRHVLICIL
                     AGGHALIEGVPGLGKTRLVKTLANVLDLKFNRIQFTPDLMPADITGTNILVEDERGRR
                     TFQFQEGPIFANFILADEIIRATPKTQAALLEAMQDRTVTVASQEYVLGPPFFVLATQ
                     NPLEMEGTYPLPEAQLDRFLFKLIVPYPTADSMTEILRRTTTSYDPVANPVTDAKTIN
                     AMARLARETPIAKHLVDFVVRLTLATHPANEIGIREVRDYVQVGVSPRGAQAIVLGAK
                     IRALLNDRLNVAMEDVLDIAAPSLRHRLILNFNAIADRVAPDEIIQRLLAELPESAVV
                     "
     gene            7073..7146
                     /locus_tag="VHR64_07450"
     tRNA            7073..7146
                     /locus_tag="VHR64_07450"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7107..7109,aa:Asp,seq:gtc)
     gene            complement(7150..>7354)
                     /locus_tag="VHR64_07455"
     CDS             complement(7150..>7354)
                     /locus_tag="VHR64_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302702.1"
                     /translation="DTIAATIRPHYIDAAGPRDVTTAHRMRSYVNAPSNCDIKQNGRE
                     VNLTAVSVVIIATTTNLSSIAMT"
BASE COUNT         1210 a         2224 c         2344 g         1576 t
ORIGIN      
        1 ggagtctcgg cggatggtgg caccgaatcg ccctacgatc gaagtaccgc ggtcgatgag
       61 accaacctgt ctcgcgttgt cctgatcaga gaggcaatcg acagcaccgg cgctaccgac
      121 atgccgatct ggttcacgca cctcggttgg accggcagca gcgactcgcc ggtctctccg
      181 gatgaccagg cgcgtttcgt ggagtctgca attcggcggg cgcggtcgga atggccgtgg
      241 gtaggcttga tgttcaactg gacgtttggt gtcgagcccg atgtcccgaa ctcagcgaat
      301 ctcgccctga tcgtgaacgg taacccgacg ccgctctatg acgcgatgtc cggctttgcg
      361 aagtcaagct tcggggccag catcacgaat ggtttcgtgc ccgcaacggc ctcggcctgc
      421 gcgtattcgg gaaactggca ggatcagcac ctggccgaag gcatctaccg caccgtgcga
      481 gacccgagtt cggtcgtgac ctgccggttt tggggcaccg ggatctcggt cttcttccgg
      541 ttcagccctg atgccggcac ggcaaggtac gcaatcgaca gcaccgatct cgattcagcg
      601 agcgatcgca acgccggtag cgtgatcctg caataccggg tgcaggatgc gttcgaggca
      661 cccgtggcgc tagcgtccgg tctgccagag ggtcagcaca ccgttacgat cgcgctcgac
      721 gattcgggcg gcgagttggt gattggcggg tttctcgtgt ctcgggagcg tccgatgatc
      781 tggccgatcg ccgtgctggt tgcggccgga ttggttgccc tgttcctggg tttgcggagc
      841 ctggcattcc tggccgccga gcacgtcgga cttgtgcagc caaaaagcga tgcgccggcc
      901 cagacgccgc tgcccatact gccggactgg aaaccggcgc cgcgattcca gcgatgacga
      961 tacgccgatt cccgcctgtt ttcgggcggc cagacatcgc gtgcatcctc gccatcctca
     1021 tttctgctcc agtcgcattc ggtatcccga ccgtcgtaaa gctcgggttg ctcctgctgg
     1081 cgacgggagt cgtggttctg ggtggaccag tggccggtgt ctcgctggtc tgcgccgcgc
     1141 tctcggtatc ggcgttcgta ttcacgatcg gctcgaccga gtggagcccg ctcgaattgg
     1201 cgctgctttc ttgcggagcg gccacgggct tcgtggtcgt gctcgagttt gcgcggaacc
     1261 ggcgattgtc ggtcatttgg aagtggatcg gaccccggga tgtcatattc atcgccgtcg
     1321 cggttctggt cgtcggggcg ctctcgatac tttgggttgc cgatgctgat cttcggtcgg
     1381 acagtctccg atcgctgcgc cgggtgatta tcgagccgct gttggtcatc ccggcgatga
     1441 ccgccgtggt cagggcacgg cagcaagcgg tcgtagcgcg ctggattgcg ggacctgccg
     1501 tgctcgttgc ggtactggcg ttagcgcaac tggcagccca gcgttcaacc gtagacatcg
     1561 gcggaatcgc ccgcccgatc ggcaccttca cgcatccgaa caatctgtcg ttttacctcg
     1621 agcgcgcgat ttggttcagc ccattcctgg cagtcccatt cgcacgtcgc ggggatcgct
     1681 ggtcatgggc agcaggggga gtgatcctgc tggcgacgat cgccacgttg tcccgaggcg
     1741 cggccatcgc gctggcagcg ggcggactgg tcatgttctg ggaggttatt cgcgcccggt
     1801 ggcggctctt cgccgggctg gccactgccg gagtggctgc catattcgca tcgcggtacc
     1861 tggccgaaac cggcgactcg atcgataccc gaaccacgat ttggcgatcg tcggtcgata
     1921 tgctgcgtga tcatccggtg accggtgtcg ggctggacca attcctgggt cagtatgggc
     1981 ggcgatatgt gcggatcgag ggctggcccg agcggtacac gagtcatccg cacaatctct
     2041 tcctcgattt ctggctgagt ctcggcctgg cgggtctggc gactctgtgg atgatcctgg
     2101 aagcaacatg gagccgcatc aggagcacgt tgggcacgag cggtccgacg gtgcatcgcg
     2161 cgggtgttgc cttgctggtc gcaggattcg cgcacgggct gatcgataac agcttcttcc
     2221 tgccgtacct cgccactatg acctggattg gcctgaccat cagcgttctt cccgggcgag
     2281 atcatggctg atctgggtcg gcttctggtc gacaacatct ggatctggac atccctggcc
     2341 ggagtgatcc ttgcagcgtt cgcatttctg ctttccgtcg acggaagcga ctgggcgccg
     2401 atcctcgtga tggcaacggt gccaattcag cggaccctgt tgatcgggac cgacggtcac
     2461 gcggcgacct ggacgcaggc cgcggtagct ggattctttg ccggtgccgc gattcgattt
     2521 ggcgccggta cattgcggat tcggctcgat gggcccgcga tcctcatggc agccgtcgtt
     2581 tcgctgtacg gcctctcgat tgcggtggcc gatgaccgtg gcctctgggc ggcggaaacc
     2641 tatcgctggg ccatcgccgg gctgtttctg atcgtcgccc gaagctactt cgatgaccga
     2701 tctgactcgc gggtcgcgtt ggcacttgcg gccggtgcgg ttgtggcatt cgcgtgggcc
     2761 gcattgcagg tggcgtccga ggaaggtcca gcgagctttg tccgcagcgg agtaatgcgt
     2821 gcgcgtggcg gattcggaga gccgaatccc tacgcggcat tcatttgggc tgtcactctc
     2881 ccgctgcttg cctttgcggt cgccggcaaa gggaagtcgc gctggattcg gtggacgagt
     2941 gcggcagtcg gctctctggg gctggcggcc ctggtcatga ctcaatcccg cggcggcatg
     3001 ctgggtattg gcgccggatt gtgcgtcatt ggcggcctgt tgttattacg gctgaaccca
     3061 cggctgaggt tgatcggcct gggcagcgta gcaactatcg cggcagcggc tatcgtgttg
     3121 gtggccatcg catcgccatg gcgcgagatc gatgcggcaa cgacgccggg aaattgggcc
     3181 gaccaggagc gaaccgcgca ctgggttgct gccgtacaca tggtcgagtc gcatccggta
     3241 accggagtcg gctcaggcga gttctccgcg aattaccggg ccgataccgg ttactggcgt
     3301 ttccggatat cacgtggaca cgcccacaat gcatacttgc aggtggccgc cgaggttgga
     3361 ttgccgggaa tgctcgcgta cgcttgtctg ctggctgcta tcctcggatc gctgatccgg
     3421 cggctgcgga cgactgatgg gagctggctg gcactcggcg tcgcggcgat gaccatggcg
     3481 atgatcgtcc atcagttggt ggactatctc cacgttttga gtttgggatt actcttcgca
     3541 ggtttgtggg cggcagccct tccttccgat tccagggaca tttcatcgcg tgaacacaac
     3601 attgcagcct gagcccctga tcgaacagac agagcctgtt atcgagcagc cggtgacatc
     3661 ggttgtcgtt gaggccccgg agccgatcca gcgacgcctc ttccgaccgc acacgattgc
     3721 gtccttcgca atcgcgattg caatcatcat cctgctggtc aaacgactga acatcaacct
     3781 cagcgcggtc tgggacaaca tcagccgggc gaacccgtgg tatttcagtg ccgccgtcat
     3841 cctctattac ctgacattca tcgcccgcac ttatcggtgg cgatacatgc tcaagcaggc
     3901 cggaatcacc cgcgaaaacg ggtatgcggt tcccggtatt cccggcttgt tcgagatcat
     3961 gatgctctcg tggtttgcca attgcgtggt gccggccaag cttggcgacg gctatcggtg
     4021 ctatctcctg aagcgcgatg ccggtgctcc cgccagcgcc acacttggca caatcctggc
     4081 cgagcgcata accgatctca ctgtcctgtt tgtcgcaatg tctgccgccg gcgtgctgac
     4141 attccatggc aacctgccgt ctcgcgttca acagacggtc atgatcggcg gcatattgct
     4201 tgtgttcgca atcgtggtac tggtcgctct gttttttgct cgcgattttg tccagcgatt
     4261 cgttcccgat cgattcgtgc gccactacca tgattttcac gacgcggtct tcgcgtgtct
     4321 gcgccaaccc gcgattccgg tcatggtcag cgttggcatc tggattgcgg acggcttccg
     4381 cctgttcctg gttgcgcgct cgctcggtgc cgatctgacc atcactctca cggtcttcgt
     4441 tgccctgatg agcgcactcc tgaccacatt gccgatcacg ccggccggat tgggcgtcgt
     4501 cgaggcggcc gtggtcgtcg tcttgaagct ggtcgatgtc catgccgatc ttgcgagctc
     4561 gatcgcggtg ctcgaccggg tcatcggcta ctggagcctg atcgcgttcg gtctgctcct
     4621 ctacctgcat cgcatgcggc gcgacgtgcg cgtggcccga gcggcatgag tcaacctcgc
     4681 ctcctgatcg attccagcgc cgggttcaat cagggagcgg gaatcgggcg atacagccgg
     4741 tccgtgctgc gggccgcgtt gccatcgctg cagtccgact tcgatatccg gctctggtat
     4801 gcatcggaca cgcgccacgg cgcgcgcttc gagcgcgagg cgatggacgc gattcctccg
     4861 tcacttcagg ggcacgtcaa gcggtcgcgc tggagccgcc ggcaggtcga ccagtttgcg
     4921 cgcttgccaa tcgatgtccc gacgcgcctg attgccggag cggccgatgt cgcctactcg
     4981 ccggacttca ccctgccagg acagttcggc ggcgcgggga tgatcactgt ccacgatgtg
     5041 gctttcgagc tgatacccga agcgtatccc gcgggccttc tccaatacct gcgaacggtg
     5101 gtgccgcgga atgtccgtcg cgccgacgtc gtcgctgtcg tctcgaggac aacgagaatc
     5161 gacgttgtcg agcgctacaa cgtcgattcc gaacgggtag tggtgattcc aaacgcggcg
     5221 gacgatcgtt tcttcggcgc gtcgtcgctt gggtccgccc gtcgtcagga gctcggcatt
     5281 cccgacgact tcttgttgac ggtcgggacg atcgagcccc gcaaaaatta ccggaccctt
     5341 cttgccgcgc agaagatcgc attccttaag accggacgcc cgctggttgt ggtcgggcgg
     5401 tcgggatggg gtaatcggga cgaaatgcag atgctaaatg agcttgcgga cgagggcgct
     5461 gtcattccgc ttctgaacgc cgatgatgcc gatcttcccg gactctattg cgatgcccga
     5521 gccgtggtct acgtgccgct ctacgaggga ttcggcctgc cggtgctcga ggcaatggcg
     5581 tctggcgccc cggtgattac cagcgatatc ccctcggtca aggaagttgc ggccggacat
     5641 gccacgatcg tcccaccgct cgacgtcgaa cagatggcgg agtcgatggc atcgccggtt
     5701 gcgagaagtg ccgaattccg cgcatcgctc agggatgccg ctcgggcgta ttcatgggac
     5761 gcatcgggta ctatcttgcg cgaagcacta accgggttgg cagggcgagc gacgtgatgg
     5821 cagacgaagg gcggtacatt ccgctcgagg aactggacga agaaacccgg cgcgaggtga
     5881 tcaagacccg gcgcgtgatc aagctcgtcg gaatcgtctc aatcggactg atcgtcctga
     5941 tggcggtctt caccatcatt gccgtcctgc tcgtctacgg tgactgatcg cgagagggtc
     6001 agatccatgg aacacgacca gtccgcatcg atcgatatcg cccactttcg cgaaacggcg
     6061 caccagatcg aagccgaggt ctcgcgggtc atggtcgcgc aggaggacat cgtccggcat
     6121 gtcctgatct gtatcctggc aggcggccac gcgttgatcg aaggcgtgcc ggggctcggc
     6181 aagacccgtc tggtcaagac actggccaac gtgctggatc tcaagttcaa ccgtatccag
     6241 ttcacacctg acctgatgcc ggccgacatc accggcacaa atatcctggt cgaagatgag
     6301 cgcggccggc ggacgttcca gttccaggaa ggcccgattt tcgccaactt catcctggcc
     6361 gacgagatca tccgcgcgac gccgaagacc caggcggcgc tcctcgaggc gatgcaggat
     6421 cggacggtca ccgttgcgtc ccaggagtac gtcctgggac cgccattttt cgtgctggcc
     6481 acccagaatc cgctcgaaat ggaagggacc tatcccttgc cggaagcgca gttggaccgg
     6541 ttcctgttca agctgatcgt gccatatccg actgccgaca gcatgaccga gattctgcgc
     6601 cgcacgacca cgagctacga cccggttgcg aacccggtca ccgatgcgaa gacgatcaac
     6661 gcgatggccc gactggcgcg cgagacgccg atcgcaaagc acctggtcga cttcgtggtc
     6721 cgtttgaccc tggctacgca tcccgccaac gaaattggca ttcgcgaggt gcgcgattac
     6781 gttcaggttg gcgtcagtcc acgtggcgcg caggcgatcg tgcttggggc gaagatccgg
     6841 gcgctgctaa atgatcgctt gaatgtcgcc atggaagacg ttctcgacat tgcggcgcca
     6901 tcgctccggc accggttgat cctgaacttc aacgctatcg ccgaccgcgt cgcgccggat
     6961 gagatcatcc agcgactcct cgcagagttg cctgaatctg cggtggtcta ggcgggttta
     7021 ccggcaagtt ccttgacccg tcctcgccaa aatggtagat tctaatgccg ctggaggcgt
     7081 ggtgtagtgg cctaacatgc agccctgtca aggctgagac cgcgggttcg aatcccgtcg
     7141 cttccgctct tacgtcatgg cgatagacga caggttcgta gtcgtcgcaa taatgacaac
     7201 tgaaacggcc gtgaggttca cctcacggcc gttctgtttt atgtcgcagt ttgatggagc
     7261 gttgacatac gagcgcattc gatgtgcagt cgtcacgtcg cgcgggccgg ccgcgtcaat
     7321 gtagtggggc ctgatcgttg ccgcgatcgt gtca
//
LOCUS       DATAOZ010000283         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7484964, whole genome shotgun sequence.
ACCESSION   DATAOZ010000283 DATAOZ010000000
VERSION     DATAOZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7484964"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2157
                     /locus_tag="VHR64_07460"
     CDS             <1..2157
                     /locus_tag="VHR64_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX3302703.1"
                     /translation="GSIASAGGAGLAAAQGTPGPSVPKIEFGYRKPGGPSVEQLAFQF
                     EGDVQRIFEFVRDQVRYDAYPGALRGALGTLWGLAGNSVDQAQLLAAMLNHVKIKTRF
                     VSGTLQDATASSLIDSVHVTVTAAQEHAAKVHAKQSTGDATSVAQELTPEQQKALDDL
                     ITRAQALTEDTKRQLSEGVDIIMTALSNAGVTLPTTGVALPEGEQSRHVWVQYAAGTE
                     WVDLDPSISNAAPGTAYADSVETFDDLPQEWMHAISFTLSAEKIQGGKATKSGLLTHS
                     EYAQDLVGKSLVLVHLQPDSLKGLGAVIESALEGTVQYIPHLALGTNDVVPGTDRVTL
                     VTDGGTTDAFGGERTNDGDTVSEWLEITITSPEMPDRHITREVFDRLGPELRNQPDAD
                     LSTFPTATLTDLGSQGGTVFLPLLALWSIVPTVGAVPVSYFDQDYSVVDLYGNLSLPG
                     YSYHVARDGLSIAFADTGCPRIYANRPNLTAFLLTPTNFSEDRTEGEATIDLMFQSLA
                     DIPWSGIPAPNAPAAVVAGVLGPVAERLTLESTTGNEVVKPSIASVSALFEEVARQSI
                     PVVALLPGKPVPSTVSLSNRARVLVTEALDSGYIVVVPEQPVSLGGEALTGWWQIDAA
                     TAETFDRMETGLGMTTLGYADTVKVSVQATPAMRLLSICLFAAGFLASMMLGAIFSGT
                     ALAAGAQGGLAAFAIGGGVASLVGGGASGALVAAAC"
     gene            2256..>2548
                     /locus_tag="VHR64_07465"
     CDS             2256..>2548
                     /locus_tag="VHR64_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302704.1"
                     /translation="MNGDRFDQLARLVARLADCRQSRRSILRTAAAGAGATVLAASHG
                     SAAPDRRSAIRARNQEAGTDPLIDETAFNLEYDLGKIFQFVRDEVGYDPYAGV"
BASE COUNT          494 a          788 c          783 g          483 t
ORIGIN      
        1 gggtcaattg catccgctgg cggcgctgga ttggcagcag cccagggtac accgggcccg
       61 tctgttccga aaatcgagtt cgggtatcgc aagcccggcg gaccgtcggt cgagcagctc
      121 gccttccagt tcgagggcga tgtccagcga atcttcgagt tcgttcgcga tcaggttcgc
      181 tacgacgctt acccgggtgc gctccgtggc gcgctcggca cactttgggg cctcgccggc
      241 aactccgtcg atcaggcaca actcctggcc gcgatgttga atcacgtgaa gatcaagacc
      301 cggtttgtgt cgggaacact tcaggacgcg acagcatcct ctctgatcga cagcgtacat
      361 gtcacggtga cggctgccca ggagcacgcc gcaaaggttc acgccaagca gtcgaccggc
      421 gacgcaacgt cggttgcaca ggaactgaca cccgagcagc agaaggcgct cgacgacctg
      481 atcacacgcg cccaggccct cactgaagac acgaaacggc aactgtcgga gggtgtggac
      541 atcatcatga ctgcgctttc caacgcaggt gtaacccttc cgactaccgg agttgctctg
      601 cctgagggag agcagtcccg tcacgtttgg gtgcagtacg ccgccggcac cgaatgggtc
      661 gatctggatc cgtcaatctc gaatgcagcg ccgggaaccg cttacgccga ctcggtcgag
      721 acgttcgacg atttgccgca ggagtggatg catgcgatct cattcacact ctcggccgag
      781 aagatccagg gtggaaaggc gacgaagtcc gggctgctga cgcattccga atacgctcag
      841 gatctcgtcg ggaagtccct ggtgctcgtg cacctgcaac cggattcgct caagggattg
      901 ggtgcggtga tcgagtccgc acttgaaggc accgttcaat acattcccca cctcgccttg
      961 gggacaaatg acgtcgtccc cggaacggac cgcgtgaccc tggtcacgga tggcgggacg
     1021 acggatgcat ttggcggcga gagaaccaat gacggggaca cagtcagcga gtggctggag
     1081 atcacgatca cttcgccgga gatgcccgat cgccacatta cccgtgaagt cttcgaccgt
     1141 cttggccccg agctccgaaa ccagcccgat gccgacctga gcacgttccc gacagcgacc
     1201 ctgacagatc tgggaagcca gggtggtacg gtgttcctgc cactgcttgc actctggtcg
     1261 atcgtgccga cagtcggcgc ggtaccggta agctacttcg accaggatta ttcagtcgta
     1321 gacctgtacg gcaatctcag cctgccggga tatagctacc acgttgcccg cgatggacta
     1381 tcgattgcgt ttgctgacac aggctgccca cgtatctatg ccaaccgccc gaacctgacg
     1441 gcatttctgc taactccgac caatttttcg gaggaccgta ccgagggcga ggcgaccatc
     1501 gatctcatgt tccagagcct cgcggacatt ccatggtcgg ggattccggc tccaaatgca
     1561 ccggccgccg tcgtcgctgg cgtgctcggc ccggtcgcgg agcgcctgac actggagagc
     1621 actactggta acgaagtggt caagccgtcg atagcaagcg tcagcgcgct cttcgaagag
     1681 gtagcccggc aatcgatccc agtcgtcgcc ctccttcccg gcaaaccggt gccatcgaca
     1741 gtgtcgctgt cgaatcgggc aagggtgtta gtcaccgagg cgctggacag cggatacatc
     1801 gtggtcgtgc ccgagcaacc cgtctcgctc ggcggagaag ccttgaccgg ttggtggcaa
     1861 atcgacgctg caaccgcgga gacctttgac cgcatggaga ccggcctggg aatgaccacg
     1921 ctgggatatg ccgatacggt caaagtaagc gtgcaggcaa cgcctgctat gcgactgttg
     1981 agcatttgcc tcttcgcagc aggattcctc gcttcgatga tgctcggcgc aatattttcc
     2041 ggcaccgcac tggctgcagg ggcacaaggg ggcctcgctg cgttcgccat aggtggagga
     2101 gtagcctcgc ttgtcggcgg aggggcctcc ggcgcgcttg tcgcagcggc ctgttgacgt
     2161 tcgtcgacac aacctgtcag ttccatcgga gctgacgaca cagtctcgta gttgccgtag
     2221 agaaggcatt tcgcacgagt cgtcaggagc aacggatgaa cggcgaccgg ttcgatcaac
     2281 ttgcgcgtct ggttgcccgg ctggccgatt gccggcaatc acggcggtcc atcctgcgaa
     2341 ctgccgctgc cggcgctggc gcgaccgtcc tggccgcttc acatggctct gctgcccccg
     2401 atcgcagatc cgcgatccgc gcccgaaacc aggaagcggg aaccgacccg ctgatcgacg
     2461 agaccgcgtt caatcttgag tacgatctgg gcaagatctt ccagttcgtt cgggacgagg
     2521 ttggctacga cccatacgcg ggcgtctt
//
LOCUS       DATAOZ010000284        15034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5642128, whole genome shotgun sequence.
ACCESSION   DATAOZ010000284 DATAOZ010000000
VERSION     DATAOZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15034
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5642128"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /gene="secF"
                     /locus_tag="VHR64_07470"
     CDS             <1..578
                     /gene="secF"
                     /locus_tag="VHR64_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX3302705.1"
                     /translation="RRSFFAVGLASIGILLYIAYAFRNTQNPVLYGIAAIVGMLHDVL
                     VVLGIFSIMGKVAGTEIDSLFVTALLTIIGFSVHDTIVVFDRIRENLARRVSPSFEEV
                     VNYSLAQTIVRSLNTSMTVVFTLLALYLFGGESTKEFVLALLIGVVSGTFSSIFNASQ
                     LLVSWETGEIQRLWERIKNFGRAPAPAPSGR"
     gene            821..2971
                     /locus_tag="VHR64_07475"
                     /pseudo
     CDS             821..2971
                     /locus_tag="VHR64_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
     gene            3059..4393
                     /locus_tag="VHR64_07480"
     CDS             3059..4393
                     /locus_tag="VHR64_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3302706.1"
                     /translation="MSETPGQKQIGIVGGGYAGITAALRLAQRGHKVTLWERAPQFGG
                     QAIAFPVPGNYLEHFYHHLFQSDKEITALIHELGIGDKLMWLPSNVGYFADGKTYPLN
                     GALDLLKLGIIPIHDRVRLGLVTAYLQRVKDWHKFEKVTAETWLKRALGERAYKRTLG
                     AQLDAKFGKYQHDVAMVWFWGKIWLRTTSRRSPLDQEKLGYIKGSFNVVTDAIVDGAR
                     KAGAELNSGAALTGLTPLPDDRWQVQFEDRPAVEVDSVIVTTPSPIMLRLAPMLPDWY
                     KQILSGLTYEAAAVALLQLDRKLSDIYWLNIADPDMPFTGVIEHTNFMPPEDYDGKHY
                     VYLSKYMEPDHPYFSMPEEELIKTYLPYLKRINPDFDESWITKWWVFRERAAQPIVTL
                     NYREKLPDYRTPLKSLYLANTSQIYPEDRGTNYSVRLGNQIAELVNEDLTGP"
     gene            complement(4390..5283)
                     /locus_tag="VHR64_07485"
     CDS             complement(4390..5283)
                     /locus_tag="VHR64_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3302707.1"
                     /translation="MALVQLSDQVSVLPGGVNIGVVRIDDRQAALIDSGLNDTSAKKA
                     IKAAREELGAEVVAVITTHGHADHFGGNATVVKRTGARVFAPALDEVILRYPILQPAF
                     LYGGADPIDSMRTNFMLADASPVDVVYEAGPLEVDGVSLQAVSLAGHSGNQMGIMVDG
                     IFFAADVVLPESILEKYRIPYLYSVTDHLKALDACADVPARHVVPGHGQMLDDISNLR
                     DRNRAIVTETAEVIVDFCNLPRTSGEIMKYVLDARGANVVDSPGYYLLQPTINAYLTH
                     LIRTGELSHEVAANSSTWRRA"
     gene            5352..6263
                     /gene="pyrB"
                     /locus_tag="VHR64_07490"
     CDS             5352..6263
                     /gene="pyrB"
                     /locus_tag="VHR64_07490"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX3302708.1"
                     /translation="MKPQHIVEVSQFDRDFVESVFASADRMRDLPRPSTLQAGKILAT
                     IFYEPSTRTRLSFESGMLRLGGQVISTENAREFSSAIKGETVEDTVMMASGYADAIVI
                     RHYEQGAAARAAAVSPVPILNAGDGPGEHPTQALLDLYTIQHELGRIDNLRIALVGDL
                     RYGRTARSLALLSRLTTGVELVFVSPAQVPMGDDIRAALDKAGVPYHDEPDLAKVLPD
                     VDVVYQTRVQKERFGSEEEYRAVKGTYILDRAMYDRLNPTAVVMHPLPRVDEITTDVD
                     SDPRSAYFRQARNGVYVRMALLDLVLG"
     gene            complement(6260..7738)
                     /locus_tag="VHR64_07495"
     CDS             complement(6260..7738)
                     /locus_tag="VHR64_07495"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3302709.1"
                     /translation="MSATIDQETDTLPEPDGAGAIAVEDALSDVELTPSRTNGSLFRN
                     FPFLRLWGAQALSMTASNMVNFALLLRVREIIEHHGLKQANTLTSLEILAFSLPSVFI
                     GPLAGVVADRFDRRLVMATTNGVRAVAMALFLIIDPSWHVQSILIATYLVTVVFGTAG
                     QFFAPAEAATIPDLVPRTQLISANGMFNLTNTGSQLAGFALLGTLLVKLFGVNAVFWT
                     ALVIFLACACIIMTLPKSKPSEARARKATNEQPMRRLWLDIREGLVFILRDPYLIKAI
                     LHLSIAAMTLQMLAALGPEFVTSILGLAPEDLLFIVGPAATGVFVGVLLVGPTTRRYD
                     RGSVIDWALTAAGVAMFLLVAAEPVLSWIGNPSHNTLTLITAFFAACLGVCNAYVLIP
                     AQTMLQERSNENIRARVYATFFTISNTLAFVPIVFAAALADVFGVVQILICIAILIGL
                     IGGQSVARRRLDEKRRWMRVRRRHREGPESISPGSGRRGMPL"
     gene            complement(7735..8010)
                     /locus_tag="VHR64_07500"
     CDS             complement(7735..8010)
                     /locus_tag="VHR64_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEX3302710.1"
                     /translation="MKRLQNEPLQLYLGDVIRLKKPHACGGFDWAVTRLGVDIGLRCT
                     TCSRRIMLPRLETERRFKQFVERGDPSTALDPTASTPSHAPDVESGA"
     gene            complement(8043..8375)
                     /locus_tag="VHR64_07505"
     CDS             complement(8043..8375)
                     /locus_tag="VHR64_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX3302711.1"
                     /translation="MFNYAKGTNLAMGINMKAIQQMQMRLEKVQKELGETIVEGSAGG
                     GVVVAKVTGQKDFQSIKIDPSAVDPDDVEMLEDMVNAAITDAMTKAGALMEEKMGALT
                     GGLKIPGL"
     gene            complement(8445..10142)
                     /gene="recN"
                     /locus_tag="VHR64_07510"
     CDS             complement(8445..10142)
                     /gene="recN"
                     /locus_tag="VHR64_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX3302712.1"
                     /translation="MLAELAIRNFAIIDELHLQFDDGMNALTGETGAGKSIIIDALGA
                     VLGERVGSEVVRTGASAATTEAVFDLADRDASEITALLDELGIEDEDGTLILSRQIAA
                     GGRSSARINGRPVTVGALNQLGALLIDIHGQSDHLKLLQAGAQLDALDHYAGTMGDRE
                     HVRELVRELHAVRSARNRLRTGEREREQRVDLLKYQVDEISSAALEPDEDERIANERA
                     VLANAERLIGDAAAALQALEPDDDDAGAASLLRSALQHVQRIAEVDASTAELSERINE
                     VVVLADDVASDLRRYVESVDADPARLASVEERFDLIQKLKRKYGATIPEIQAHLEAAQ
                     AELETLTGAGFDEAALAEQETELEATLAPVLDALSTARRGGADRLETAIDAAIAELGL
                     GSAHVSIAISTLPDPTETGADAVEFLFAPNKGEALKPLAKIASGGETARLMLALKSVL
                     AEGDRTPTLVFDEVDVGVGARSGQSVGEKLWSLANRHQVIVISHLAQIAAFAGAHFMI
                     EKDVAGDRTVSTVRRLEGGTRLDELAEMIDGKPVSPESRAAAEQLMDRISSIKRTGG"
     gene            complement(10146..11117)
                     /locus_tag="VHR64_07515"
     CDS             complement(10146..11117)
                     /locus_tag="VHR64_07515"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX3302713.1"
                     /translation="MQTATTSVQTSLSGAYGGEKWSPRTTTFREDIVDAWGDWGSGSE
                     CGTLRAVLLRRPGPELDQIEDFDAVQMRADLDPERARAQHDAMAAAYESHGVTVYQVE
                     NGRMDKPNSFFLRDLMLMTPEGAIITRPASTVRAGEERLVAEALGRIGVPILMTVHGS
                     GTFEGADVSWVNEDLCFLAEGLRTNEEGANQVERMLREIGVKDVIRVGLPYGAMHLDG
                     LLNFPDRDCAVVWARRTPFKIVDTLKQRGFRILEVPDEVEAQTCLPMNFVALKPGEIL
                     MPTGGDALRRIYEAAGIVCHTVEIDELIKAGGGIHCMTAFLKREPLI"
     gene            complement(11117..13393)
                     /locus_tag="VHR64_07520"
     CDS             complement(11117..13393)
                     /locus_tag="VHR64_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3302714.1"
                     /translation="MIDSTMTGHRLLGRSSKRIDGERKITGAEKYTADLRLRGMLFAR
                     PVTSPLPHARVRSIDKDAALAIPGVVAVLTAEDLPIRRPLSSLPGKSPLAFGEIAFAG
                     QFVAIVLAESDQAARDAVELVEVDYEELAAVPDFETGVDESSPLARVDATDSNSDEAA
                     AHNADAATQTAEAERPSSANVSSTLKFERGDVNAGFAQAVKTAEVTIRSESVHQGYIE
                     PQVAMASIDPIGEITIYTSTQGAFLARQRAADWLGRPVGEINVKTMAVGGGFGGKFIL
                     LEPLTAALAVAVNRPVLLEFGRTDDLAASNPAPACEITVKVGADADGRLTALQGDLTF
                     DTGSQAGSPLQIAAILLGGYYKVENLQITGREVVTNRAPAGAYRAPGAQQATYAIESA
                     IDDLAEALGDDPLEFRLRNCVEEGDVRPNGGAWPRIGLRATLEALRDHPAWKDRQAGT
                     GIAIGGWPGGVEPATSICRLDHDGKFTVVVGSADISGVNTGFQKIAAEVLGLNEEDVV
                     VTGADTSAAPYAGASGGSKVTYTVGAAVMKAAQEARAQVLQIAAQHLEASADDLEIVD
                     GKVQVRGVPSAGVSLKEIASMSMSFAGKYEPVYGRGQTAITESAPGFAAHLANVTVDE
                     ETGEVHLNRYVAAQDVGFAINPALVEGQMMGGVAQGIGWALHEAIEFDDSGQLVTGTL
                     MDYVLPRSNDIPPIETILVEVASQHGPFGAKGVGEPPAIPGPATIRNAIKAASGARLS
                     SLPMRPERVLEALQERGQ"
     gene            complement(13471..13773)
                     /locus_tag="VHR64_07525"
     CDS             complement(13471..13773)
                     /locus_tag="VHR64_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302715.1"
                     /translation="MPLYLVEHIPYDEDVESISPPTDLGGLARYSVDATHGARWLTTF
                     SPDLHDDRHFSIWDAPDAESIHAVMARFGFLSDGIVKAFHVRQWGPEDVLSEHAPQ"
     gene            complement(13776..14888)
                     /locus_tag="VHR64_07530"
     CDS             complement(13776..14888)
                     /locus_tag="VHR64_07530"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEX3302716.1"
                     /translation="MADPVTDTTPIQPSGTDREALIDNARKQARRRFKERLDSGLSVA
                     GCECESDNGDSDGPTSRVAGVRFLDSGQTYFFDPGSFEIENGDWVVVETNRGLEAGKV
                     VIAPSQMLQASLSGNLKPINRILGEPEIERMQALKREAAKAVKTFGAKIREHNLPMKP
                     ISAEYNFDGSHLTLNFTAPERVDFRELARDLGSSFRCRVELRQVGPRDEARLLGGVGR
                     CGRTLCCASWLPVFPEVSMNMAKTQDLPLNPSKVSGVCGRLLCCLSYENDQYKQQKNL
                     LPKLGQPVQTPMGDGMVVSLQVLRELVTVRYQDETPDQTFTASDLGFQAKPERPKNEG
                     PRGGAEGSAEGPARRRRRRGRGRGQSAQSDQTEAQT"
BASE COUNT         2687 a         4915 c         4648 g         2784 t
ORIGIN      
        1 cgcgccgatc cttcttcgcg gtcggtctcg catcgatcgg catcctgctc tacatcgcct
       61 acgcattccg gaatacgcaa aacccggtcc tgtacggcat agccgcgatc gtaggcatgc
      121 tccacgacgt gctggttgtg ctcggcattt tttcgattat gggcaaggtt gccggaacgg
      181 agattgactc cctgttcgtg accgcgttgc tcacgattat tggtttctcg gttcacgaca
      241 ccatcgtcgt cttcgaccga atccgcgaga acctggcccg ccgcgtgtcg ccgtcgttcg
      301 aagaggtcgt caattacagt cttgcacaga cgatcgtgcg gtccctcaac acatcgatga
      361 cggtcgtctt cacgttgctg gccctctatc tcttcggtgg cgaatcgacc aaggagttcg
      421 tgcttgccct tctgatcggc gtcgtcagcg gcaccttttc ttcgatcttc aacgcgagcc
      481 agttgctggt gtcgtgggag acgggcgaaa tccagcgcct ctgggagcgg atcaagaatt
      541 tcggtcgcgc gccggcgccg gctccaagtg gccgataacg ccatagcccg cgttattgct
      601 atctgataaa cttcggtact gcgcgtcggg cgtacgatcg ctcttcgcgc agttttgtgt
      661 gcgatccccg ttcacgaatc cggatcagac ctggcatgca ctcagtgatg aatggtgcct
      721 gatcgggacg cacaggtaag gaaccagcca ccgctgcagc ggcacgaatc aatgcagcac
      781 accttcaggg gagggtcagt tggacgagca agcgctctat atggtgccgg tcgtgctttt
      841 cggcatcatt gcgatcctgt atgccgtttg gttggcgaag gacgtcatgt cacgcgacca
      901 gggcactccg gatatgcaaa aggtctccgg agccattttc cagggcgcca aggcttacct
      961 gaaccgtcaa taccgcacga tcggccaatt ggcgatcgtc gtcgcggtcg tgatgggaat
     1021 tctcgtcgcg atatttgaga aagaccatca ggccgagcgc ggcgtcatca cagcactcgc
     1081 cttcctgatc ggcgcgtccc tctctggcat ctccgggtac atcggcatgt acgtggcagt
     1141 ccgcagcaac atccggaccg ccgcggctgc gcgcaagggc atgggcgagg cgctcgtcgt
     1201 cgcccttcgc ggtggcgcgg tctcgggctt cctggtcgtc gcgctcggcc tgctcggcat
     1261 cagcggtgtc tactggctgg tctcactctt cgccgaaggt gagaacgccg cgatcgccga
     1321 gaccccattc tggatcgtcg gcttcgcgtt cggcgcgagc ttcgttgctc tcttcgccca
     1381 gctcggcggc ggcatctata ccaaggcggc tgatgtcggc gcggacctgg tcggaaaggt
     1441 cgaggccggt atccctgagg acgatccacg caatccggcc gtgatcgctg accttgtggg
     1501 cgacaacgtc ggtgactgcg ccggccgtgg cgccgacctc ttcgagtcat cggccgcgga
     1561 gatcatcggc gcgatgatcc tgggcgtagc cctgttcaag gcatccggtg ccgcgaccgg
     1621 caccagcgat gtcgcctgga tcttcttccc gctcgttctc gcggccttcg gcgcaatctg
     1681 ctcgatcgtc ggcgtctcgg tagtgcggcc gagcggctac ctggcgaatc cgatgggcga
     1741 gctcaacaag ggcttcttcc tgacgattgc gctcgcggcc gtcgctatct ttgtggtctg
     1801 cttctggatg ctgccgtacg gagcattcga gtacgcgatc tgcggcatca tcggtctcgt
     1861 gacctcgctc gcattcctgt tgatcacgca gtactacacg gcgggcacct atcgtccggt
     1921 tcgcgacatc tcgaacgcat caaagcaggg ctccgcgacc aatatcatct ccggcatcgc
     1981 ggtcggtatg gagacgacag cccttccggc cctgactgtt ggcgcggcgc ttctttcctc
     2041 ctattacatc ggcaagacgg tcgactttga tacgactgtc cagttttcac tgttcaactc
     2101 gggtggcatc tacggcaccg cggtcgcgac gatgggcatg ctgatgaccg ccgcctatat
     2161 cctcgccatg gactgcttcg gcccgatcac ggacaacgcc ggcggcattg tcgaaatgag
     2221 caaccagccc gaggaggtcc gcgacgtcac cgatgcgctt gactccgtcg gcaacacgac
     2281 caaggcgctg accaagggct atgccgtcgg gactgccggc ctggccgcat tcctgctcct
     2341 ctccgcctac ctggatgagg tacggcggtt cagtgggaac gacaccctcg gtcgaattgt
     2401 gaatcttgcc gagccgaagg tcttcgtggg cgggttgatc ggcgctgccc tgacgttcct
     2461 ctttgcctcg tttgcgatcc gcgccgtcgg ccgggccgcg cagggcatga tcgatgaggt
     2521 ccgccgccag ttccgggcag atccgggcat catggccggc acatctgagc cggactacgg
     2581 ccgctgagtc gacatctcgg ttcagagtgc gctccgcgag atgatcctgc cgggcgccct
     2641 ggcggttgcc gttccgatcg gcgtcggcgt gatcctccgc tgggaggccg cggccggcat
     2701 gctgatggtc ggcacgatcg ccggcctcct gctcgccgtc atgctcaata atggtggtgg
     2761 cgcgtgggac aacgccaaga agttcatcga agccggacat ctcaaggatg acaatggcaa
     2821 tgtcctgggc aagggcagcg acgcccatgc cgccgcggtg gtcggcgaca ccgtcggcga
     2881 tccctggaag gacacagcag gtccgtcgct ccacgttctg atcaagctcc tggcgacgat
     2941 caccctggtg ctggccccgc tgttcatcta ggacctaccc acccaatttg atactggtga
     3001 tagccgagcg gtcttgctac agtccgctcg gttatcgttt aggcgtgatt gaggggacat
     3061 gagcgaaaca ccgggacaga agcagatcgg catcgtcggc ggcggctatg ccgggatcac
     3121 ggcagcgctc aggctggccc agcgcggtca caaggtgacg ctctgggagc gggcaccgca
     3181 gttcggcggc caggccatcg cgttcccggt gccgggcaac tacctcgagc acttctatca
     3241 ccacctcttc cagagcgaca aggagatcac ggcgctgatc cacgagcttg ggatcggtga
     3301 caagttgatg tggctgccgt ccaatgtcgg ttacttcgcg gacggcaaga cgtacccgct
     3361 caacggcgcg ctcgacctgc tcaagctcgg catcatcccg atacacgatc gcgtacggct
     3421 gggactcgtc actgcctacc tgcagcgcgt caaggactgg cacaagtttg agaaagtgac
     3481 ggccgagacg tggctcaagc gggcgcttgg cgagcgagcc tacaagcgga ccctcggcgc
     3541 ccagctcgat gccaagttcg gcaaatacca gcacgatgtc gcgatggtct ggttctgggg
     3601 caagatctgg cttcgcacga cgagccggcg atcgccgctc gaccaggaga agctcggcta
     3661 catcaaaggc agcttcaacg tcgtgactga cgcgattgtc gacggcgcgc gcaaggcggg
     3721 cgccgagctg aactcaggcg ccgctctgac cggtctgaca ccgctgcccg acgatcgctg
     3781 gcaggtccag ttcgaggatc ggccggctgt ggaggtcgac tcagtgatcg tgacgacgcc
     3841 ctcgccgatc atgctccggc tcgcgccgat gctgcccgac tggtacaagc agatcctgtc
     3901 cggactaacc tacgaagcgg ctgccgtcgc gctgctgcaa ctcgatcgca agctgagcga
     3961 tatctactgg ctcaacatcg ccgacccgga catgcccttc accggcgtga tcgagcacac
     4021 caacttcatg ccacccgagg actacgacgg caagcattac gtctacctca gcaagtacat
     4081 ggagccggat cacccctatt tctcaatgcc ggaagaggag ttgatcaaga cctatctacc
     4141 gtacctgaag cggatcaatc ctgacttcga cgagtcatgg atcacgaagt ggtgggtttt
     4201 ccgcgagagg gccgcgcagc cgatcgtcac gctcaactac cgcgaaaaac tgccggacta
     4261 ccgcacaccg ctcaagagtc tctacctggc caataccagc cagatctacc ccgaggaccg
     4321 cggcacgaac tacagcgtcc gcctcggcaa ccaaatcgcc gagttggtca acgaggatct
     4381 gaccggtccc taggcacgtc gccaggtcga actatttgcg gctacttcat gcgacagttc
     4441 accggtccgg atcaaatgtg tcaggtacgc gttgatggtc ggctgaagca ggtaatagcc
     4501 cggcgagtcg accacattgg ctccgcgcgc gtcgagcacg tacttcatga tctccccgct
     4561 ggtgcgcggc agattgcaaa aatcgacgat gacctcagca gtctcggtca cgatggcccg
     4621 gttgcgatcg cgaaggttgg agatgtcgtc cagcatctgc ccatgccccg ggacgacgtg
     4681 tcgcgccggg acgtccgcgc atgcgtccaa cgctttcagg tggtcggtga ccgaatagag
     4741 atacgggatt cgatacttct ccaggatcga ctcaggcagc acgacatctg ccgcgaagaa
     4801 gatcccgtcg accatgatcc ccatctggtt gcccgagtgc ccagccagtg acaccgcctg
     4861 caacgacacg ccgtcgacct ccagcggtcc ggcttcgtag accacatcga ccgggctggc
     4921 gtcggccagc atgaagttgg tcctcatcga atcgatcgga tcggctccgc catagaggaa
     4981 cgcgggctgg agaatcggat agcgcagtat tacctcgtcg agcgccggtg caaaaacgcg
     5041 cgcgccggtg cgcttgacga cggtcgcgtt cccgccgaag tggtccgcat ggccgtgggt
     5101 cgtgatgact gcgacgacct cggcaccgag ctcctcccgg gcggccttga tcgccttctt
     5161 cgccgatgtg tcgttgaggc cggaatcaat caacgcggcc tgccggtcat cgatccggac
     5221 aacgccgata tttacgccac cgggaagcac agagacctga tccgacaact gaacgagtgc
     5281 caagtttcac ctcctgcgcc actatacttt ccggccggag aggtccgcgt acatcccgga
     5341 ggcacaacca attgaagccc cagcacatcg tcgaggtgag ccagttcgat cgtgacttcg
     5401 tcgagtcggt ctttgcctcg gcggaccgga tgcgcgatct gcctcgcccg tccacgctcc
     5461 aggcgggcaa gattctggct acgatctttt acgagccatc gacgcggacc aggctcagtt
     5521 tcgagtcggg catgctccgg ctcggcggtc aagttatctc gaccgagaac gcgcgcgagt
     5581 tctcctcggc catcaagggc gaaacagtag aagacactgt catgatggcg agcggctatg
     5641 ccgacgcgat cgtgattcgc cattacgaac agggcgcggc cgcgagggcc gccgccgtct
     5701 cgccggtgcc gatcctgaat gcgggcgacg gacccggcga gcatccgact caggcgctgc
     5761 tcgacctcta cacaatccag cacgagctcg gcaggatcga caacctgcgg atcgcgctgg
     5821 tcggcgatct ccgctatgga cggacggcgc gctcgcttgc gctcctctcg cggctgacga
     5881 ccggcgtcga gctcgtcttc gtcagcccgg cccaggtgcc gatgggcgac gatatccggg
     5941 ccgcgctcga caaggccggc gtcccctacc acgacgagcc ggacctcgcg aaggtgctcc
     6001 cggacgtcga cgtggtctac cagacacgcg tccagaagga gcgcttcggg agcgaggagg
     6061 aatatcgcgc ggtcaagggg acgtacattc tcgatcgcgc gatgtacgac cggctcaacc
     6121 ctacggcggt cgtcatgcat ccgctgccgc gcgtggacga gatcacgacc gacgtcgatt
     6181 ctgatccgcg ctccgcctac tttcggcagg cgcggaacgg tgtctatgtg cggatggcct
     6241 tgctggattt ggtgctcggt tagagcggca tgcctcgccg gccggagcca ggagagatgc
     6301 tctctggtcc ctcccggtgg cgtcgacgga cgcgcatcca gcgccgcttc tcgtcgagcc
     6361 ggcgacgagc gacactttgc cctccgatca gcccgatgag gatcgcaatg cagatcagga
     6421 tctggaccac gccgaagacg tcggccagtg ctgcggcgaa gacgatcggc acaaacgcga
     6481 gggtgtttga gatcgtaaag aacgtcgcat agacccgggc ccgaatgttc tcgttcgagc
     6541 gctcctggag catcgtctgc gccgggatca ggacgtacgc gttgcaaacg cccaggcacg
     6601 ccgcaaagaa ggcggtgatc agcgtcaggg tattgtgcga agggttgcca atccagctta
     6661 gaacgggttc ggctgcaacc agcagaaaca tcgcgacgcc ggccgccgtc aatgcccagt
     6721 caattactga accacgatcg tacctgcgcg tggtcggacc gacgaggagc acaccgacaa
     6781 acacaccggt cgcggccggt ccgacgatga agagcaggtc ctccggtgct agcccgagaa
     6841 tgctcgtcac gaattctgga cccagggcgg ccagcatctg gagcgtcatc gcggcgatgc
     6901 ttaggtgcaa gatagcctta atcagatagg ggtcgcgcag gatgaagacg agcccctcgc
     6961 ggatatccag ccagagccgc cgcatcggtt gttcgttcgt cgccttgcgg gcgcgcgctt
     7021 cgctcggctt cgatttcggc agcgtcatga tgatgcatgc gcatgccagg aagatcacca
     7081 atgcggtcca aaagaccgca ttcaccccga acagcttgac caacaacgtg ccaagcagcg
     7141 caaacccggc aagctgcgag ccggtgttgg tcagattgaa cattccgttc gcggagataa
     7201 gctgggtgcg cggaaccaga tccggaatgg tcgcggcctc cgccggcgcg aagaactggc
     7261 cggccgttcc gaacacgacg gtcaccaggt aggttgcgat caggatcgac tgcacatgcc
     7321 aggacggatc gatgatcagg aagagcgcca tggccacagc acgaacgcca ttcgttgtgg
     7381 ccatcaccag tcggcgatcg aaccggtcgg ccacgactcc ggccaacggc ccgatgaaaa
     7441 ctgacgggag cgagaatgcc aggatttcga ggcttgttag cgtgttcgcc tgcttgagcc
     7501 catgatgctc gatgatctcg cgcacccgga gcaggagggc gaagttgacc atgttgctgg
     7561 cggtcatgga aagcgcctgc gctccccaca aacgcaagaa cgggaaattg cggaagagcg
     7621 acccgttcgt ccggctcggt gtcagctcga catccgagag cgcatcttct accgcaatgg
     7681 cgccggctcc atccggttcc ggcagggtat cggtttcctg gtcaatggtc gcactcatgc
     7741 cccggactcc acgtccggag catgcgaggg cgttgatgcg gtgggatcga gcgcggtcga
     7801 cgggtcgccg cgctcgacga actgcttgaa ccgccgctcg gtctcgagtc gtgggagcat
     7861 gatccgacgg gagcaggttg tacagcgcag accaatgtcc acacccaggc gggtcaccgc
     7921 ccagtcaaag ccaccgcagg catgcggctt cttgaggcgg atcacgtcgc ccaggtagag
     7981 ctggagtggc tcgttctgca accgcttcat gccggcccgc tccggaggat gcgaactggt
     8041 agctagagac cgggaatctt caggccgccg gtcagagcgc ccatcttctc ttccatcaac
     8101 gcgccggcct tggtcatggc gtccgtgatg gcggcgttga ccatgtcctc gagcatctcg
     8161 acatcgtccg gatcgaccgc ggacggatcg atcttgatag actggaaatc cttctggccg
     8221 gtgactttgg caacgacgac accgccgcca gcgctgccct caacgatcgt ctcgcccagc
     8281 tctttctgga ccttctccag ccgcatctgc atttgctgga ttgccttcat gttgatgccc
     8341 atggcgagat tcgttccttt cgcgtaattg aacatgcgtt gaatgcctgt tgaacaacga
     8401 acctgagtat aggcgagcga tgcgcgcggc cgggcatccc aatctcaccc gccggtccgc
     8461 tttatcgatg agatccggtc catgagctgc tcggcggcgg cgcgcgattc cggagagacc
     8521 ggtttgccat cgatcatctc ggccaactcg tcgagccgag tcccgccctc cagccgccgc
     8581 accgtcgaga ccgtccgatc gccggcgacg tccttctcaa tcatgaagtg cgccccggca
     8641 aaggccgcga tctgcgccag gtggctgatc acgatcacct ggtggcgatt ggcgagcgac
     8701 cagagcttct cgccaacgga ctggcccgac cgcgcgccga caccgacatc gacctcatcg
     8761 aagaccaggg tcggagtccg atcgccctcc gccaggaccg atttcagggc aagcatcagg
     8821 cgagccgtct cgccgccgga ggcgatcttc gcaagcggct tgagcgcttc gcccttgttc
     8881 ggcgcaaaca ggaactcgac cgcgtcggct ccggtctctg tcggatcagg caatgtcgaa
     8941 atagcgatgc tgacgtgggc agaaccgagc ccgagctcgg cgatcgcggc gtcgatcgcg
     9001 gtctccagac ggtctgcccc gccgcgccgc gccgtcgaca gcgcatcgag cacgggcgcg
     9061 agtgtcgcct ccagctctgt ctcctgctcc gccagcgccg cctcgtcgaa gccggcgccg
     9121 gtcagcgtct ccaattccgc ctgtgccgct tccaggtggg cctggatctc ggggatcgtc
     9181 gccccatact tgcgcttgag cttctggatc agatcgaagc gctcttcgac cgaagcgagt
     9241 cgggccgggt cggcatccac cgactcgacg tatcggcgca ggtccgaggc gacatcgtcg
     9301 gccaggacca cgacctcgtt gatgcgctca ctcagctccg ccgtcgacgc atcgacctca
     9361 gcgatccgct ggacatgctg gagtgcggag cgaagcaggc tcgccgcgcc ggcgtcgtca
     9421 tcgtcgggct cgagcgcctg caatgccgcc gccgcgtccc ctatgagccg ttcggcattc
     9481 gcgaggaccg cgcgctcgtt ggcgatgcgc tcgtcttcgt caggctcgag tgccgccgac
     9541 gagatctcat cgacctggta cttgagcaag tcgacacgct gctcccgctc ccgctcgccc
     9601 gtgcgcaacc gattccgagc agagcggacc gcgtgcagct cgcggacgag ctcacgcacg
     9661 tgttcgcgat ctcccatcgt gcccgcgtaa tgatcaaggg cgtctagctg tgcgccggcc
     9721 tggaggagct tgagatggtc ggactggcca tggatgtcga taaggagcgc gccaagctgg
     9781 ttgagcgctc caaccgtcac cggccggccg ttgatccggg ccgaagatcg gccaccggcc
     9841 gcgatctggc gagagagtat cagcgtgccg tcctcatcct cgatccccaa ctcgtccagc
     9901 aaggcggtaa tctcgctcgc atcccgatcg gcaagatcga agacggcctc ggtcgtcgcg
     9961 gccgaggcgc cggttcgcac gacctctgac ccgacgcgct caccgagcac agcccctagc
    10021 gcgtcaatga tgatcgattt gccggcgccg gtctcgcccg tcaatgcgtt catgccgtca
    10081 tcgaactgca aatgcagctc atcgatgatt gcgaagttgc ggatggcgag ttcggccagc
    10141 atgctctaga tgagcggctc ccgtttcaga aacgcagtca tgcaatggat gccgccaccg
    10201 gccttgatca gctcatcgat ctcgacggtg tgacagacaa ttccggccgc ctcatagatt
    10261 cgccgcagcg cgtcaccgcc ggtcggcatc agaatctcgc ccggcttcag cgcgacgaaa
    10321 ttcatcggca ggcatgtctg cgcctcgact tcgtccggaa cctcgaggat ccggaacccg
    10381 cgctgcttga gcgtatcgac gattttgaac ggcgtccggc gtgcccagac gaccgcgcag
    10441 tcccgatccg ggaagttgag caggccatca aggtgcatcg cgccatacgg aagcccgacc
    10501 cggatcacgt ccttgacacc gatctcgcga agcatccgct cgacctggtt cgcgccttcc
    10561 tcattggtgc ggagtccctc ggccaggaag caaaggtcct cgttcaccca cgagacatca
    10621 gcgccttcga aggtgccgct gccgtggacg gtcatcagga taggtacgcc gatccggccg
    10681 agcgcctcag cgaccaaccg ctcctccccc gcccgaacgg tcgacgccgg ccgggtgatg
    10741 atcgctccct ccggcgtcat cagcatcagg tcgcgcagga agaacgagtt cggcttgtcc
    10801 atcctgccgt tctcgacctg gtagacagtc acaccgtgtg actcatatgc agccgccatg
    10861 gcgtcgtgct gggctcgcgc ccgctcgggg tcaagatcgg ctcgcatctg gaccgcgtcg
    10921 aagtcctcga tctgatccag ctcggggccc ggtcggcgca ggagcaccgc acgcaacgtg
    10981 ccgcactccg agcccgagcc ccagtcgccc catgcgtcga ctatgtcctc gcggaatgtt
    11041 gtcgtacgcg gcgaccactt ctcgccaccg taggctccgc taagagacgt ctggactgat
    11101 gttgttgcgg tctgcattac tgtccgcgct cctgcagggc ttcgagaacg cgctccgggc
    11161 gcatcggcag ggacgagagt ctcgcgccac tggcagcctt gattgcattc cggatcgtcg
    11221 ccggtcccgg gatcgccggt ggctcgccaa cgcccttggc cccgaacgga ccgtgctggc
    11281 tcgcgacctc gaccaggatc gtctcgatcg gcgggatgtc gttgctgcgc ggcaggacgt
    11341 agtccatgag cgtgccggta acgagctggc ccgagtcgtc gaattcgatc gcctcatgca
    11401 gcgcccagcc tatgccctgt gcaacaccgc ccatcatctg cccttcgacc agggcggggt
    11461 tgatcgcgaa gccgacatcc tgggcagcga cgtaccggtt gagatggacc tcgccggtct
    11521 cctcatcaac ggtcacgttg gcgaggtgcg ccgcgaaacc gggcgcgctt tcggtaatgg
    11581 ccgtttggcc gcgcccgtac accggctcgt acttgccggc gaaggacatc gacatgctcg
    11641 cgatctcctt gagcgacacg ccggcactgg gcacgcccct aacctgaacc ttgccgtcga
    11701 cgatctcgag atcgtcagcg ctggcttcga ggtgctgggc agctatctgc aggacttgcg
    11761 ccctcgcctc ctgagccgcc ttcatgacgg cggcgccgac cgtgtatgta accttcgacc
    11821 cgccgctcgc gcccgcgtat ggcgcggcgg aggtgtcagc accggtcacg acgacgtcct
    11881 cctcgttcag gccgagaact tccgccgcaa ttttctggaa gccggtgttg acgccgctga
    11941 tgtcggccga cccaaccacg accgtgaact tgccatcgtg gtccaggcga caaatcgacg
    12001 tagccggctc gaccccaccc ggccagccgc cgatcgcgat gccggtcccc gcttggcgat
    12061 ccttccaggc cggatggtcg cgcagcgcct cgagcgtggc cctgagtccg atccgcggcc
    12121 aggcgccgcc gttcggccgg acatcgccct cttcgacgca gttgcggagc cggaactcga
    12181 gcggatcgtc gccgagcgcc tcggccaggt catcgatcgc cgactcgatt gcataggtcg
    12241 cctgctgagc gcccggggcc cggtacgcgc cggccggagc ccggttcgtg acgacttcgc
    12301 gcccggtaat ctggaggttc tcgaccttgt agtagccacc gagcagaatc gccgcaatct
    12361 ggagtggtga gccggcctgc gatcccgtgt caaaggtcaa atcgccctgc agtgccgtca
    12421 ggcggccgtc ggcatccgcg ccgaccttga cggtgatctc gcaggcaggc gccgggttcg
    12481 aggcagccag gtcatcggtg cgtccgaact ccagcaagac gggacggttc accgcaaccg
    12541 ccaatgcggc cgtcaggggc tcgagcagaa tgaattttcc accgaatccg ccgcccacgg
    12601 ccatcgtctt gacgttgatc tcgccaaccg gccggcccag ccagtcagcg gcgcgctggc
    12661 gagccaggaa cgcgccctgc gtgctggtgt aaatcgtgat ctcaccgatc ggatcgatcg
    12721 acgccatcgc cacctgcggc tcgatatagc cctggtggac cgactcggac cggatcgtca
    12781 cctcggccgt cttgactgcc tgagcgaagc cggcattgac atcgccccgc tcgaacttga
    12841 gcgtcgagga gacattcgcc gacgatggcc gctcagcctc ggccgtttgc gtcgccgcat
    12901 ctgcgttgtg agccgcggct tcgtcgctat tcgaatcggt cgcgtcgacg cgtgcgagag
    12961 gtgacgattc gtcgacgccg gtctcgaagt ccgggaccgc cgccaactcc tcgtagtcga
    13021 cctcgaccaa ctcgaccgca tcacgcgcgg cctggtcgga ctcggcgagc acgatcgcga
    13081 cgaattgccc ggcgaaggca atctcgccga aggcgagcgg cgacttgccc ggcaaagagg
    13141 agagcgggcg ccgaatgggc agatcctcgg ccgtcagcac ggcgaccacg ccgggaatgg
    13201 caagcgccgc gtccttgtcg atcgagcgga cccgggcgtg cggaagcggg ctggtcaccg
    13261 gccgtgcgaa aagcatgccc cgcaagcgca gatccgccgt gtatttctca gccccggtga
    13321 tctttcgctc cccatcgatg cgcttcgatg agcgacccaa gagccggtgg ccggtcatag
    13381 tcgagtcaat cattcagttg ctccggaaag tacggcgggt gcagatgtcg cccctgccac
    13441 gaacagcgta gggggattct accaacaggg ttactgcggc gcgtgctcgg aaagtacgtc
    13501 ctctgggccc cactgtcgga catggaacgc cttgacgatc ccatcactga ggaatccgaa
    13561 gcgggccatg accgcgtgga tcgactcagc atcgggagca tcccagatgc tgaagtggcg
    13621 atcatcgtgc aaatcaggcg aaaaggtcgt cagccaacgt gccccgtggg tcgcgtcgac
    13681 cgagtagcgg gcgagcccgc cgagatccgt cggtggcgag atcgactcca catcttcgtc
    13741 gtatgggatg tgctcgacca ggtagagggg cattgctatg tctgcgcttc tgtctggtcc
    13801 gactgcgccg attgcccgcg accgcgaccc cgccggcgcc ggcgacgcgc cggtccttcg
    13861 gctgagccct cggctcctcc acgcggtcct tcgttcttcg gccgctctgg cttggcctgg
    13921 aaaccgaggt cggatgcggt gaaggtttgg tccggcgtct catcctggta gcgtacggtc
    13981 accagctcgc gaagaacctg gagcgagacg accatgccgt cccccatcgg cgtctggacc
    14041 ggttggccga gctttggcag gagattcttc tgctgcttgt actggtcgtt ctcgtacgac
    14101 aggcagcaaa gcaatcgacc gcagacgccg gaaaccttgc tcgggttgag cggcaggtcc
    14161 tgcgtcttgg ccatgttcat cgagacctcc gggaagaccg gaagccacga cgcgcagcag
    14221 agcgtccgcc cacagcggcc cacaccgccc aacagcctgg cctcgtcgcg cggaccgacc
    14281 tgacgcaact caacgcgaca gcgaaagctc gatccgaggt cccgggccaa ctcgcggaag
    14341 tcgacgcgct ccggcgccgt aaagttgagc gtcagatgcg agccgtcgaa gttgtactcg
    14401 gccgaaattg gcttcatcgg caggttgtgt tcgcggatct tcgccccaaa tgtcttgact
    14461 gccttggcgg cttcccgctt gagcgcctgc atccgctcga tctcaggctc gccgagaatg
    14521 cggttgatcg gcttgagatt gcccgacagc gacgcctgca acatctgtga cggcgcgatg
    14581 accaccttgc ccgcctcgag gccccggttg gtctcgacga caacccagtc gccgttctcg
    14641 atctcgaacg agcccggatc gaagaagtag gtctggccgg aatcgaggaa gcgcacgccg
    14701 gccacccgcg aggtcgggcc gtccgagtcg ccattatctg attcgcattc gcagccggcc
    14761 acggacagcc cggaatcgag ccgctccttg aagcgacgac gcgcctgttt gcgggcgtta
    14821 tcgatcaatg cctcgcggtc ggtacctgac ggctgaatgg gagttgtatc ggttacagga
    14881 tcggccattg attcaccata gcttgcattg cgagtctggg tcgaatgttt ccggtcaagt
    14941 cctgaatgca ctgccgggta gcggataacg cgcgatgcca ctcgctgata tcggctgacg
    15001 accgcgagat caacgatcgc gcgcgaatgg catc
//
LOCUS       DATAOZ010000285         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5906368, whole genome shotgun sequence.
ACCESSION   DATAOZ010000285 DATAOZ010000000
VERSION     DATAOZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5906368"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VHR64_07535"
     CDS             complement(<1..372)
                     /locus_tag="VHR64_07535"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302717.1"
                     /translation="MPSPVPVDVEYMVQATAELCRIPSPTGYTHLVTERLEQMLGELG
                     LTATTTVKGSLVVALPGPAEGDGRLLSAHVDTLGAMVKEIKPSGRLKLTMIGGYDWST
                     IEGEFCLVHRADGNPVSGTIVT"
     gene            complement(394..1962)
                     /locus_tag="VHR64_07540"
     CDS             complement(394..1962)
                     /locus_tag="VHR64_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3302718.1"
                     /translation="MAQTVEKITYTATAEQVEQMHAAFDDALVEARELLGKTYPMIIN
                     GEERTGGKTFTATSPIDRSIVIGHFQSGTAKDVDDAVAAAKAAYPAWSGRPWQERVAV
                     MRKAASLVRERKFLLSALLIIECGKIRAEAIGEVEEGADLIDEYANQMEANNGFARTM
                     ASLDPCEQNQSMLRPFGVWAVLAPFNFPHALSAGMSSGALVAGNTVVFKPASATPLAG
                     YELARCYVDAGVPAGVFNFITGSGSEVGERLAMHEDVDGVIFTGSKDVGLDLFKRFST
                     DFPKPCITEMGGKNPTIVTAKADIDKAVDGVARSAFGFSGQKCSACSRVYVEQPVYDE
                     FVTKLTDRAKMLKVGNPEERDVYVGPVIDDKAVDRFEQAVSKAKGGTIRAGGSRLTEG
                     EYGKGTFVQPTVVDGLPANHELFRKELFLPFVVVAPVKSLDEALTLSNDTEYGLTAGI
                     FTEDEGEIDQFFDGIEAGVVYANRRGGATTGAWPGCQSFCGWKASGSTGKGGLGPYYV
                     QQFLREQSRTRVIE"
     gene            complement(1966..>2345)
                     /locus_tag="VHR64_07545"
     CDS             complement(1966..>2345)
                     /locus_tag="VHR64_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3302719.1"
                     /translation="VQHVSIPMPPGGAEEARAFFGGALGLVEKQVPASLDASALTWFS
                     VGDDEHELHCYVDPDYENRSSAAHFCFEVEDIEALRTRVREAGIVFDHDPAPIHNRPR
                     SFVRDPFGNLIEFTQINGRYEEG"
BASE COUNT          423 a          809 c          683 g          430 t
ORIGIN      
        1 agtcacgatg gtgccggata cgggattccc gtcggctcga tgcacgagac aaaactcgcc
       61 ttcgatcgtc gaccagtcgt acccgccgat catcgtcagc ttcagccgcc cgctcggctt
      121 gatctccttg accatcgctc caagtgtgtc gacgtgggct gacaacagtc gcccatctcc
      181 ctcggccgga cccggcaacg ccacaaccag cgagcccttg acggtagtcg tcgcggtaag
      241 gccaagctct ccgagcatct gctcgagacg ctcggtgaca aggtgcgtgt atcccgtcgg
      301 gctggggatg cgacagagct ctgccgtcgc ctgaaccatg tattcgacat caaccggaac
      361 tggcgacggc atgaatgctc ctgcttgtga gtgctattcg atgactctgg tgcggctctg
      421 ctcgcgcagg aattgctgga catagtaggg cccgagccca cccttaccgg tgctcccgct
      481 cgccttccag ccgcagaacg actggcatcc cggccacgcg ccggtagtcg ccccgccgcg
      541 ccggttcgca tagaccacgc cggcttcgat cccatcgaag aactggtcga tctcgccctc
      601 gtcctcggtg aagatgccag cggtcaggcc gtactcggtg tcgttcgaga gcgtgagcgc
      661 ctcgtcgagc gacttcaccg gcgccacgac cacaaacggc aggaagagct ccttccggaa
      721 cagctcgtga ttggccggca acccatcgac gacggtcggc tggacgaagg tgcccttgcc
      781 atactcgccc tcggtcagcc gcgagccacc ggcgcggatc gtgccgcctt tggccttgga
      841 caccgcctgc tcgaagcggt cgaccgcctt gtcgtcgata actggtccta cgtagacatc
      901 gcgctcttcc gggttgccga ccttgagcat tttggcccgg tcggtcaact tggtcacgaa
      961 ctcgtcatag accggctgct cgacatagac ccgcgagcag gccgagcact tctggccgga
     1021 gaagccgaag gcagaccgcg cgacgccgtc caccgccttg tcgatgtcgg cctttgcggt
     1081 cacgatcgtc gggttcttgc cgcccatctc ggtgatgcac ggcttcggga agtcggtgct
     1141 gaaccgctta aacaggtcga gcccgacatc cttcgaccca gtgaagatca caccgtcgac
     1201 gtcctcatgc atcgccagcc gctcgccgac ctcggacccg gacccggtga tgaagttgaa
     1261 cacgccggcc ggaaccccgg catccacata gcagcgcgcc agctcgtatc cggccagcgg
     1321 cgtggcagac gccggcttga agacgacggt attcccggcc acaagcgcgc cggacgacat
     1381 cccggccgag agcgcgtgcg ggaaattgaa cggcgccagc accgcccaga cgccgaacgg
     1441 ccgcaacatc gactggttct gctcacacgg atcgagcgat gccatggtcc gggcgaaccc
     1501 attgttcgcc tccatctggt tcgcatactc gtcgatcagg tcagcgccct cttcgacttc
     1561 accgattgcc tcggcccgga tcttcccgca ttcgatgatc aacagagcgg agagcaggaa
     1621 cttgcgctcg cgaaccagac tggccgcctt gcgcatgacc gcgacccgct cctgccatgg
     1681 ccggccggac cacgccggat aggccgcctt cgcagccgcg acggcgtcat cgacatcctt
     1741 cgccgtgccc gactggaaat ggccaatcac gatgctccgg tcgatcggcg aggtagcggt
     1801 gaatgtcttg ccgccggtcc gctcctcgcc attgatgatc atcggatatg tcttgccgag
     1861 cagttcgcgt gcttcaacga gcgcgtcgtc gaatgcggcg tgcatctgct cgacctgctc
     1921 ggcggtagcg gtataggtga tcttctcaac ggtctgggcc atgggtcatc cctcctcgta
     1981 tcgcccgttg atttgggtga actcgatcaa attgccgaat ggatcccgca cgaagctgcg
     2041 cgggcgattg tggatcggcg ccggatcatg atcgaacaca atcccggcct cacgtactcg
     2101 cgtccgcagc gcctcgatat cctccacttc gaaacagaag tgcgccgcgg acgaccggtt
     2161 ctcgtaatcc gggtcgacgt agcaatgcaa ctcatgctcg tcatccccaa ccgaaaacca
     2221 ggtcagcgct gaggcatcga gcgacgccgg aacctgcttc tcaaccaacc ccagcgctcc
     2281 cccgaagaag gctctcgcct cctccgctcc gccgggcggc atcggaatgc tgacgtgctg
     2341 gacgc
//
LOCUS       DATAOZ010000286         5608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3273847, whole genome shotgun sequence.
ACCESSION   DATAOZ010000286 DATAOZ010000000
VERSION     DATAOZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3273847"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VHR64_07550"
     CDS             <1..969
                     /locus_tag="VHR64_07550"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX3302720.1"
                     /translation="VILAAVMLKMGGYGMLRFNLPLFPEGSEDWAIWIVLLSVIAIIY
                     GSLVALVQPDLKKLIAYSSVSHMGFVTLGIFVPLLILKADRASGNVADVTHYLNGFNG
                     SMMVMLAHGFNTGGLFLCVGVIYERAHTRLIARFGGLASRMPVYSALFGIFMFASIGL
                     PGLSGFVGEFLVTLATLQYSKWVAFITFSVVIFAAWYMMWMFQRVIFGRQPEDMPDPH
                     DTELMPDEVTRLERAGLLGGHGHGEPLPVSGASHDAVSPDASSASHHSEPAAQSHDIE
                     WPDLNRIELWCLLPLAVLTIVFGVYPKPILDIVGPAFERILLPFMS"
     gene            1014..2564
                     /locus_tag="VHR64_07555"
     CDS             1014..2564
                     /locus_tag="VHR64_07555"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX3302721.1"
                     /translation="MDLLNLDLSSPEFSLMLPQLIVFGLAMVLILGDAFFPRRSHYTV
                     LTGISLVGYAAALISLYWQRDNSESTFRGMFRADGLTLFLSLTILSAAILSVMVSASY
                     VEFLEGRMPLGEFYVLLSFAVLGALMTAAAGDLVMIFVGIELSSLATYVLTAFAKRRE
                     TSVEGALKYFLLGIFASAILVYGMAWTYGLTGSTNLDAIASRMAEVKADGGRLETSLL
                     LALLLLVVGLGFKIAAVPFHMWTPDAYDGAPTPVTAYMSVIPKVAGFAAIIRILIQGL
                     GPLRDDWYLLLAILAVITMAFGNIVAVSQRNIKRMLAYSSIAHTGYAMVGLAAYEVSK
                     TGFGTSGSTTAADTAVSALLYYMLAYTFMNIGAFAVVAWIQHRGSGMMLEDFAGLGSR
                     QPLAAVAMAIFMVSLMGIPPLLGFYAKYYIILAAIQSDMLWLAMAVVLASAVSAYFYL
                     RVVAVMYFNPAPDTELRPFKTSLLNFGIGAMVVANIVLGVFSERVVNLADKWSNALTV
                     ASQVANLR"
     gene            2655..3311
                     /locus_tag="VHR64_07560"
     CDS             2655..3311
                     /locus_tag="VHR64_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX3302722.1"
                     /translation="MKIAQDPNELFDIVTWRGEPTGARKRRQHVHRDGDWHPAIHIWV
                     VGERDGLGFMLLQRRSLGKDTQPGALDVTVGGHLGAGETWREGLREADEEIGLRVAEQ
                     DVVFAGVRRGIFETDSGIRDHEIQHVFFARNDAPLTDYRPNPAELAGLIAISIDDTLE
                     LASGAIESASVEVLDSESRTVEPGQINRSELAQSTDRYFYRVAIAARLFLAGEKHFSI
                     "
     gene            complement(3408..3842)
                     /locus_tag="VHR64_07565"
     CDS             complement(3408..3842)
                     /locus_tag="VHR64_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX3302723.1"
                     /translation="MESNKAVVRRFIAEIWNAGNLAAANDLVHPEYTISGVGTGPDAV
                     RHNVSVYRTAFPDLEWVVEQLIAEGDWVAVRLTLRGTHLGPLGDIPASGKPVTMKEMV
                     FWRVDSGQLREIWSQGDALGLRIQIGAVPASAWDTPIRSFDD"
     gene            complement(3892..4686)
                     /locus_tag="VHR64_07570"
     CDS             complement(3892..4686)
                     /locus_tag="VHR64_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX3302724.1"
                     /translation="MLDTGARQFTRRRLLSVAAAGASTLALGRSAKASSGIMIKAPYM
                     HQGGSGANEAVNCGPTTVAMAINYSGAAAPSPYSVRATLGIDGPTDIDQWAWLLDVYG
                     VPWYPTWSQEQMAASLRKGHPIVIATWMGDLSAAGDYMVAWAQNAGWQGRYDSFAKGH
                     AMLLVGLSDDGYSYIVHDPNVFPGDATGFYSDGTPKGAYRRYSAWELWSTVANYANGM
                     GLAVVPYSQSFAPAKRIKRVKPEQDGIFTGPGGGNAPRRGEQQIDE"
     gene            complement(4819..>5608)
                     /locus_tag="VHR64_07575"
     CDS             complement(4819..>5608)
                     /locus_tag="VHR64_07575"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX3302725.1"
                     /translation="GGTYNSPTILMRSGVGPEDNLSALEIPVKVDLPGVGRNLHDHPA
                     VYVTHTGSEALKEQMSDWAEANFLPEEQTIAKLRSRYCTEGFDLHIYPESGPYAEGRT
                     TWDFTIPVACMTPEARGSLRLRSTDPTVAPVIDHNYLGDSDGHDRAVLVDGIGIAREF
                     ARHANQYGLLGEELEPGPSIAGDDALSEWVTDNVHHYFHAAGTCAMGPAQSPLAVTDA
                     RGAVHGVGGLYVADCSVMPTVPRANTNIPAVVVGMRIGRWLAGT"
BASE COUNT          915 a         1800 c         1776 g         1117 t
ORIGIN      
        1 gtcatcctgg cggccgtcat gttgaagatg ggcggctacg gcatgctccg cttcaacctg
       61 ccgcttttcc cggagggctc cgaggattgg gcgatctgga tcgtgctgct gtccgtgatt
      121 gcgatcatct acggctcgct ggtggcgcta gtgcagccgg acctgaagaa gctgatcgcc
      181 tactcgtcgg tcagccacat gggcttcgtc acactcggca ttttcgtgcc gctgctcatc
      241 ctgaaggctg atcgcgcgtc cggaaacgtc gccgatgtca cgcattacct gaacggcttc
      301 aacggctcga tgatggtcat gctggcgcat ggcttcaata cgggcggact cttcctctgc
      361 gtcggtgtga tctacgagcg cgcccacacg cggctgattg cccggttcgg cggtctcgcc
      421 tcacggatgc cggtctactc ggcgctattc ggcatcttca tgtttgcgag catcggtctg
      481 ccgggccttt cggggttcgt cggcgagttc ctggtaacgc tcgcaacact ccagtacagc
      541 aagtgggtcg ccttcatcac gttctcggtc gtgatcttcg cggcctggta catgatgtgg
      601 atgttccagc gcgtgatctt cggccgtcag ccggaagaca tgccggatcc gcatgacacc
      661 gagctgatgc cggacgaagt gacccggctc gagcgagccg gcttgctcgg tggtcatggc
      721 cacggcgagc ccctgccggt ttcaggcgca tcgcatgacg cggtgtcgcc cgacgcctcg
      781 tccgcatctc atcacagcga accagcggcg caatcgcacg acatcgagtg gccggatctg
      841 aaccggatcg agctgtggtg cttgctgcca ctggcggtgc tgaccatcgt cttcggcgtt
      901 tatccgaagc cgatcctgga tatcgtcggc ccggcatttg agcgcattct gctgccgttc
      961 atgagctagt tggagcacag aaagacccgc acgcgcggag tggagtgaac tgagtggatc
     1021 tgctcaatct cgatctgtca tcgccagagt ttagcttgat gctgccccag ctgatcgtct
     1081 tcggcctggc gatggtcctg atcctcggcg atgcgttctt cccgcgccgc agccattaca
     1141 ccgtcttgac cgggatctcg ctggttggct acgccgctgc cctgatctcg ctctactggc
     1201 agcgtgacaa ttcagaatcg accttccggg gcatgttccg ggccgatggg ctgacgctct
     1261 tcctgtcgtt gacgatcctc tcggccgcga tcctgagcgt gatggtctcg gccagctacg
     1321 tcgagttcct cgagggacgt atgccgctcg gcgagttcta cgtcctgctc tccttcgcgg
     1381 tacttggtgc gttgatgacg gctgctgccg gcgacctcgt catgatcttt gtcggcatcg
     1441 agctctcctc gcttgcgaca tacgtgctga ctgcattcgc caagcggcgc gagacctcgg
     1501 tcgagggtgc gctcaagtat ttcctgctcg gcatcttcgc cagcgcgatc ctcgtttatg
     1561 gcatggcgtg gacctatggc ctgaccggat cgaccaacct cgatgcgatc gcgtcgcgga
     1621 tggccgaggt caaggccgat ggcggccgcc tcgagacgtc gctgttgctg gccctgctcc
     1681 tgctggtcgt cggtctcggt ttcaagatcg cggccgttcc gtttcacatg tggacaccgg
     1741 atgcctacga cggcgcaccg accccggtga ccgcctacat gtcggtcatc cccaaggttg
     1801 ccggcttcgc ggcgatcatc cggatcctga tccagggcct gggcccgctc cgggacgact
     1861 ggtatctcct gctggccatc ctcgccgtga tcaccatggc ctttggcaac atcgtcgcgg
     1921 tctcccagcg caacatcaag cggatgctcg cctactcgtc gattgcccat accggctatg
     1981 cgatggtcgg cttggcggcg tacgaggtga gcaagaccgg gttcggcaca tccggatcta
     2041 cgaccgctgc cgacaccgcc gtcagcgcgc tgctctatta catgctcgcg tacacattca
     2101 tgaacatcgg cgccttcgcc gtcgtcgcct ggatccagca tcggggcagc ggcatgatgc
     2161 tggaggactt cgccggtctc ggcagccggc aacccctggc cgcggtggca atggccattt
     2221 tcatggtttc gctgatgggc atcccgccgc tgctgggctt ctacgcgaag tactacatca
     2281 tcctggcggc cattcagtcg gacatgctct ggctggccat ggcggtcgtt ctggcctcgg
     2341 ccgtcagcgc ctacttctac ctgcgcgtcg tggcggtcat gtacttcaac ccggcgcctg
     2401 acacggagct ccgcccgttc aagaccagcc tgctcaattt cggtattggg gcaatggtcg
     2461 tcgccaacat cgtgctcggt gtcttctccg aacgggtcgt caacctggcc gacaaatgga
     2521 gcaatgcgct gactgtggcc agccaggtcg caaaccttag ataacgcgta ggtcattgct
     2581 ctgacatcat cccgcgggcg ttcgagcaat cgaacgcccg tttcttatgt acccgttgct
     2641 gatggagtag cccgatgaag attgcgcagg acccgaacga gctgttcgac atcgtcacct
     2701 ggcgcggcga gccgaccggt gctcgcaagc ggcggcagca tgtccaccgc gacggggatt
     2761 ggcaccccgc gatccatatc tgggtagtcg gcgagcgcga tggcctcgga ttcatgcttc
     2821 tccagcgccg cagcctgggc aaggacacgc aaccgggcgc gctcgacgtg accgtcggcg
     2881 gacatctcgg tgccggtgag acgtggcggg aagggctgcg cgaggcggat gaggagatcg
     2941 ggctcagagt cgcggagcag gatgtcgtct tcgccggtgt gcggcgcggc atcttcgaga
     3001 ccgactcggg catccgcgac cacgagattc agcacgtctt cttcgcccgg aacgacgcac
     3061 cgctgactga ctatcgaccg aacccggccg agttggccgg cctgatcgcg atctcgatcg
     3121 atgacacgct cgagcttgcc tcaggcgcga tcgagtcggc gtcggtggag gtcctggact
     3181 ccgagtcgcg aacagtggag cccgggcaga tcaatcgatc ggagttggcg cagtcgactg
     3241 accgctattt ctaccgcgtc gcaatcgccg cccgcctctt cctcgccggc gaaaagcact
     3301 tcagcatctg aaactcgttc tggctcgacc gtggataagt ggagacgaag cgcggggtta
     3361 ccacgccccg cttgatgcat ccccgcgcta cctttgggtg catgcgatca atcgtcgaag
     3421 gagcggatgg gcgtgtccca ggcgctggcc gggaccgccc cgatctggat gcgcaggccg
     3481 agcgcatccc cctgcgacca gatttcgcgg agctgccccg aatcaacgcg ccagaagacc
     3541 atctctttca tggtcaccgg cttgccgctc gccggaatat cgccgagtgg accgagatgc
     3601 gtaccgcgca acgttagccg caccgcgacc cagtcgccct ccgcgatgag ttgctcgacc
     3661 acccattcga gatcgggaaa tgccgtccgg tagaccgaga cattgtgccg aacggcatca
     3721 ggacccgtgc cgacgcccga gatggtgtat tccgggtgga ccagatcatt ggcggcagcc
     3781 aggtttcccg cgttccagat ctcggcgatg aagcggcgca cgaccgcctt gttggactcc
     3841 atggacaccg gacgtttccc tccggctcaa tcgagctgtg ccggcagccg actactcgtc
     3901 gatttgctgc tctccgcgac gcggagcatt gccaccgccc ggaccggtga agatgccatc
     3961 ctgctccggc ttgacccgct tgatccgctt ggccggcgcg aacgactgcg agtagggtac
     4021 gacggcgagt cccatcccgt tcgcatagtt ggcaaccgtg gaccacagct cccaggcgct
     4081 ataccggcga taggcgcctt tcggtgtccc gtcgctgtag aagccggtcg cgtcgccggg
     4141 gaagacattg gggtcatgga cgatgtagct gtacccgtcg tcgctgagcc cgacgagcag
     4201 catcgcgtgg cctttggcaa agctgtcgta tcggccctgc cagccggcat tctgggccca
     4261 cgccaccatg tagtcacccg ccgccgacaa atcgcccatc cacgtcgcga tcacgatcgg
     4321 gtggcccttg cggagggacg ccgccatctg ctcctgcgac caggtcggat accacggcac
     4381 gccatagaca tcgagcagcc acgcccactg gtcgatatcg gttgggccgt cgatgccgag
     4441 cgtggcccgc acgctatatg gcgacggagc ggccgccccg ctgtaattga tcgccatcgc
     4501 caccgtggtc gggccgcaat tgacggcctc gttggctccg ctgccgccct ggtgcatgta
     4561 gggcgccttg atcatgatgc cgctcgacgc cttggctgac ctgccgaggg cgagcgtgga
     4621 ggcgccggct gctgccacag acagcaagcg acggcgcgtg aattgacgtg cgccggtatc
     4681 caacaccgtg gaacctccat gcagatgcaa aggtttccgc agattcagtc tctcacaacg
     4741 agcgtgaaat ctgaatcccc ggtcggtcta cgggttgcgg ggaagatggg cgcgcgtcag
     4801 tatgacctat tggggagatc aggtgccagc aagccagcgg ccgatccgca tgccgacgac
     4861 aaccgcgggg atgttcgtgt tggcgcgggg gaccgttggc atgaccgagc aatcggcaac
     4921 gtacaacccg ccaaccccgt ggacggcgcc acgcgcatcg gtcacggcga gcgggctctg
     4981 cgccggtccc atcgcgcagg tgccggcggc gtggaagtag tggtggacat tgtccgtgac
     5041 ccattcggac agagcgtcat cgcccgcaat cgacgggccg ggctccagtt cctcgccgag
     5101 gagtccgtac tggttggcgt gacgggcgaa ctcccgcgcg atcccaatcc cgtccaccag
     5161 cacggcgcgg tcgtgtccat ccgaatcgcc gaggtagttg tggtcgatga cgggggcgac
     5221 ggtcgggtcg gttgatcgca gacgaagcga tccgcgcgct tctggcgtca tgcaggcgac
     5281 cgggatggtg aagtcccagg tcgttcgccc ctcggcatag gggccagatt ccgggtagat
     5341 gtgaaggtcg aagccctcgg tgcagtaccg ggagcggagt ttggcgatcg tctgctcctc
     5401 gggcaggaag ttcgcctccg cccagtcgga catttgctcc ttgagcgcct ccgagccggt
     5461 gtgcgtgaca tagacggcag ggtgatcatg caggttccgg ccgacgcccg ggaggtcgac
     5521 cttgaccggt atttcgagcg cgctcaggtt gtcctccggg ccgacgccgg agcgcatcag
     5581 gatcgtgggc gagttatacg tgccaccg
//
LOCUS       DATAOZ010000287         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1432605, whole genome shotgun sequence.
ACCESSION   DATAOZ010000287 DATAOZ010000000
VERSION     DATAOZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432605"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VHR64_07580"
     CDS             complement(<1..1111)
                     /locus_tag="VHR64_07580"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX3302726.1"
                     /translation="MPVNAAMTGAGGFDFSWIPIHSKSPQGVQAKLAIHSPGDAAERE
                     ADHVAEQLMREPQPPATPAHDGTGRPSPFASPTLTRVQSRDPIGVDVPPSVHDVLRAS
                     SAPLDRAIGSAFASRLGHDFSHVRVHTDAAAAQSARQINARAYTVGNHLVFGEAQFSP
                     GTPSGQRLLAHELTHVVQQNASAAGVQRAPGPNAKDKEDVTAARQRAKALADRIKREG
                     TLTAAAKATLIADLKFFEGSAWEAYNEVIRPALQAVTTTDASESQKTPYWLREAAPGM
                     AALADAVTDTFFPGADLQDARFVLSVVHDAVKASQTPDAIRTDKARAAFRRRHSSHSA
                     EVLQNIDRALKRVTRDNPDLLIAYYEYYAKHELTDS"
     gene            complement(1312..1944)
                     /locus_tag="VHR64_07585"
     CDS             complement(1312..1944)
                     /locus_tag="VHR64_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3302727.1"
                     /translation="MLPLFKLGRRPGLATYLGLGLGAASVIVLFRPDVTAGQGMTAGL
                     LAVLLGSVSWAVGIVLSRKLQAPRDGAPDTSTLNAALPLICGAVMLLGGAVLRGELHD
                     FHWTAVSRESFGGLMFLVFFGSLVAFTAYAWLLKHYPPTLVATHTYVNPIVAVLLGWL
                     FAGERISPSIVASTALAIAAIGLVQQGEQQNEAYRQGHAPSRVIPNRRVR"
BASE COUNT          346 a          603 c          658 g          361 t
ORIGIN      
        1 aactgtcggt gagttcgtgc ttcgcgtaat attcataata cgcgatcagc agatccggat
       61 tgtctctcgt gactcgtttc agggcacgat cgatgttctg cagtacctcc gcagagtgac
      121 tgctgtgtct tcttcgaaac gcggcgcgag ccttgtccgt tcggatggcg tcgggggttt
      181 ggctggcttt aaccgcatcg tggaccacac tcaggacgaa ccgggcatcc tgcagatcag
      241 cgccggggaa gaacgtgtcg gtaaccgcgt ccgccagcgc ggccatgccg ggagcagcct
      301 cacgcagcca gtacggcgtc ttttgggatt cgctggcgtc ggttgtggtg acggcctgca
      361 acgcgggacg gatgacttcg ttgtaggctt cccaggccga gccctcaaag aatttcaggt
      421 ccgcgatcag cgtggctttc gcggccgcgg tgagggtgcc ttctcttttt atgcgatctg
      481 ccagcgcctt cgcccgttga cgggccgcgg tcacatcttc cttgtccttg gcgttcggtc
      541 ctggtgcgcg ctgcacgcct gcggcgctcg cattctgctg tacgacgtgg gtcagttcgt
      601 gggccagcaa acgctgcccc gagggcgtgc ccggcgagaa ctgggcctcg ccgaagacca
      661 ggtgattgcc cacggtgtag gcccgggcgt tgatttgccg ggccgactgc gccgcggcgg
      721 catccgtgtg gacgcgaaca tgtgagaaat cgtgaccgag acgcgaggcg aaggcggagc
      781 cgatggcgcg gtcgagcggt gcgctcgagg cgcgcagaac gtcatgcacc gaaggcggaa
      841 catcgactcc aattggatca cgtgattgga cccgggtcag agtgggcgac gcgaagggac
      901 tcggccgccc cgttccatcg tgagcgggcg tggcgggagg ctgcggctca cgcatgagtt
      961 gctcggcgac gtggtccgcc tcgcgttcgg cggcatcgcc gggcgaatga atcgccagct
     1021 tcgcctggac gccctgcggg gacttcgagt ggatggggat ccagctgaaa tcaaatccgc
     1081 cggcgccagt cattgccgcg ttgacgggca ttcccgcgta tgcccgggct ggagtgagat
     1141 cgggcgacgc gtgcgatggg cgaacagatg tggcctgggc ctgatcgcgc tcgggcgaag
     1201 caacggatgt cggacgctgg gttgtccttg gttgatggca gagataaccc atccctgatc
     1261 tcccttcttt ccggcggcag aggggggcag cgaggtacct tctcgatctc gctatcgaac
     1321 ccggcgattg ggaatcaccc ttgatggtgc gtggccttgc cggtacgctt cgttctgctg
     1381 ctcgccctgt tgcactaacc cgatcgccgc gatagccaat gcggtcgacg caacgatcga
     1441 cgggctgatc cgctcaccgg caaacaacca tccgagcagc accgccacga tcggattcac
     1501 ataggtgtgg gtcgctacca gcgtcggcgg gtagtgcttc agcagccacg cgtaggcggt
     1561 gaacgccacc aatgagccga agaagaccag gaacatcagt ccgccgaagg attcacgcga
     1621 caccgccgtc cagtggaagt cgtgaagctc gccgcgcagg actgcgccac ccaggagcat
     1681 cacggcaccg caaatcagtg gtagcgcggc attcaacgtc gaggtgtccg gcgcgccatc
     1741 gcggggtgcc tgcagcttgc gggaaaggac gattcccacc gcccatgaca cggagcccag
     1801 caggaccgcg agcagtcccg ccgtcatccc ctgtccagcg gtaacgtcgg gccggaagag
     1861 aacgatcacg cttgccgctc caagccccag gccgagatag gtcgcgaggc ccggccgacg
     1921 tcccaatttg aacaatggca gcatcagcgc cacgaagatc ggttcggt
//
LOCUS       DATAOZ010000288         5309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9063138, whole genome shotgun sequence.
ACCESSION   DATAOZ010000288 DATAOZ010000000
VERSION     DATAOZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9063138"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VHR64_07590"
     CDS             <1..618
                     /locus_tag="VHR64_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3302728.1"
                     /translation="IGAGVNAAAQHSGVHYSVFTHMPGIKCVVPSTPADARGLLLSAI
                     RDDDLVMFFENKVLYATKGEVPGGDEGIPLGVADIKREGSDVTIVAISRMVHQSLAAA
                     EALSAGGIETEIVDPRTISPLDEETILESVRKTHRLVVVDEDNPRCSVATDIAAMVAD
                     KGFDYLDAPIKIVTAPHTPVPFSPPLEQAYVPSPEKIAETVRQLF"
     gene            641..1966
                     /locus_tag="VHR64_07595"
     CDS             641..1966
                     /locus_tag="VHR64_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3302729.1"
                     /translation="MATVVKMPKWGLTMTAGTVTGWLYDEGAEISEGDPIFTVETEKA
                     VNDVEAPADGVLVKIVASQGDEVPVSGPVAIIAAPGEAISEAELAALIAEAAPKKSSA
                     ATTAAAAGGARAARVASRDESGRVNASPAARRRAQELGVDLATIEATGPDGRITSDDV
                     ERAAAEASADPTPREERIRLDDGRELNVLLAGTGDAPPLVFLHGLGGSQSTWQVVLGD
                     LVEQHRVAAIDLPGHGTSDRSAEADYSITGIAHAIAEAVGKLGLSRPIVVGHSLGGAV
                     ALRIAIEQPKLIQGVVALDSAGLGEEIGSELTDLMAGEAGTDTARGLLQLFYQDQKLV
                     NERGVAEMAQMQLADGAWAAQQSVANAAFASGRQLQAAHLDPAAVSVPVLLIWGEHDR
                     VIPARHAIEALTRFPDASLAIVPNVGHVPQVENPNRTATLITRFTRSLG"
     gene            2119..3705
                     /locus_tag="VHR64_07600"
     CDS             2119..3705
                     /locus_tag="VHR64_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302730.1"
                     /translation="MGASGFGQSRWRSRFLLFGLLFLLLSPVAFVAARSFVTLTDPSP
                     AVGRAQVVTQGISELPGTEYVMRVVRRTAPALGDAKVGRRVLGFALATDEPILITDVD
                     GDGTFKDRARLAPGEAYMTLDGQRQIRASLSGKPTQYLSVEFVPPDRSSDIGTGELLF
                     VSDTITTPPGQRDVDLVRNVLAQQDIAKVPDTGGQIMVLATDGAIDILPTRTRMRRLE
                     SGQSAVFNAEEIEIRPSETSAYGVPTNQLGALTDMLQTDNPVAGYVVVVIGPEVPKTG
                     EPTPSPTQPAASATIAAPTITAVAERGSIGVTGRLCNPGVTIETISDDNCPVIPNGYD
                     LALSGATLNRTLADASQVDSSWYWSDLPLGTYALTATGYPGRATDYFIPGSAAVGGSS
                     ASGYTVTLDQSSPNIVLPVYFLQGRAQPTSSAVTFTIQACQQGLVNCASPSQYNVDPQ
                     PYLVGPNGQTLTAADAQRSGDAYTWTLPAGTWSFYQQGWQSYYYVDGQTIDGGKPYVF
                     TTDGSTAASHYVQDEIIVIQ"
     gene            complement(3706..5115)
                     /locus_tag="VHR64_07605"
     CDS             complement(3706..5115)
                     /locus_tag="VHR64_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX3302731.1"
                     /translation="MAGDDFAYLSAGELIGGYSSGAISPVEVSEAMLARIDRLNPQLN
                     AFTDFTGDIARAAAKKAEADYRAGAAGPLSGVPVTIKDITFIEGLRCRRGSKIYADDA
                     PASFSSPFVQRVLDAGAVLLGQTTTPELGWKGETTSPVSGTTRNPWNLERTPGGSSGG
                     AGACIAAGIGTMAHGTDGAGSIRIPSGFSGIFGLKPSIGLVPVFPASGVADLAYHGPM
                     TWWVRDAALMLNVVAGADPRDRTSWDSNVDYVDALADLDLRGLRVAWSQDLGYAAIEP
                     EIAALAENAAKVFADLGAEVVDDHPNLPDPWPIEHVLWVAAMAGGRRDDFDQVRDIMD
                     PGLADLVEQGQKFTAADVARARTDQGIYAAAWADWLRDYDLLLTPTLPCPAFPVGENY
                     PATINGRETSYLSWTAFTYPFNLNGLPAATVPCGFASDGLPAGLQIVGRLKDDALVLR
                     AAAAFEQARPWQGTRPPLA"
BASE COUNT          970 a         1798 c         1681 g          860 t
ORIGIN      
        1 atcggcgccg gcgtcaatgc ggccgcccag cactctggcg ttcactactc cgtcttcacg
       61 cacatgcccg gcatcaagtg cgtcgtgccc tcgaccccgg ccgatgcccg gggcctgctc
      121 ctgagcgcga tccgggacga cgacctggtc atgttcttcg agaacaaggt gctctacgcg
      181 acgaagggcg aggtcccggg gggtgacgag gggattccgc tcggcgtggc ggatatcaag
      241 cgggagggat cggacgtcac gatcgtcgcg atttcccgga tggtccacca gtcgcttgcc
      301 gcggccgaag cgctctccgc cggggggatc gaaaccgaga tcgtcgatcc acgcaccatc
      361 tccccgctcg acgaggaaac gatcctggaa tcggtccgca agacgcaccg acttgttgtt
      421 gttgatgaag acaacccccg atgtagcgtt gcgaccgata tcgccgcgat ggtcgccgac
      481 aagggcttcg actatctgga cgcgccgatc aagatcgtga ccgcgccgca cacgccggtc
      541 ccgttcagcc cgccgctgga gcaggcctac gtgccgagtc ccgagaagat tgccgaaacc
      601 gttcggcagc tcttctagtt cgacaccggg aggagcgcac atggctactg tcgtcaagat
      661 gccgaaatgg ggcctgacga tgaccgcagg cacggtcacg ggatggctct acgatgaggg
      721 cgccgagatc tccgaaggcg atcccatctt cactgtcgaa accgagaagg ccgtcaacga
      781 cgtcgaagcg ccggccgacg gcgtgcttgt caagatcgtc gcctcgcagg gcgacgaagt
      841 gccggtcagc ggtccggtcg ccatcatcgc ggcgcccggc gaagcgatca gcgaggctga
      901 gctagccgcg ctgatcgcgg aggcagcacc gaagaagtcg tcggccgcga ctactgccgc
      961 agcggccgga ggagcacgag ccgcgcgagt ggccagccgg gatgagtccg gtcgggtcaa
     1021 tgcgtcgccc gccgcgcgcc ggcgcgcgca ggagctcggt gtcgacctcg ccacgattga
     1081 ggcgacggga cccgacggcc gtattaccag cgacgatgtc gaacgcgccg cggccgaggc
     1141 aagcgccgat cctactccgc gcgaggaacg aatcagactc gatgacggtc gcgagctcaa
     1201 tgtcctgctc gcgggcacag gcgacgcccc accgctcgta ttcctgcacg gactcggtgg
     1261 atcgcaaagc acgtggcagg tggtcctcgg cgacctggtc gagcagcacc gggtcgccgc
     1321 gatcgacctg cccggccacg gcaccagcga cagatcagcc gaagccgatt actcgattac
     1381 cgggatcgcc catgcgatcg ccgaggcggt cggcaagctc gggctctcga ggccgatcgt
     1441 ggtcggccat tcgctcggcg gcgcagtcgc gctcaggatc gcgattgagc aaccgaagct
     1501 gattcagggt gttgttgccc tcgacagcgc cggacttggc gaggagatcg gcagcgagct
     1561 gaccgatctg atggctggtg aggcgggaac ggacaccgca cggggtctcc tccagctctt
     1621 ctaccaggat cagaagctcg tcaacgaacg aggagtcgcg gagatggccc agatgcagtt
     1681 ggcagacggc gcgtgggccg cgcagcagtc ggtcgcaaac gccgcgttcg ccagcggacg
     1741 ccagctgcag gctgcccacc tcgatccggc cgcggtctca gttccggttc tgctgatctg
     1801 gggggagcat gaccgagtga tccccgccag gcacgcgatc gaagcactca cgcgattccc
     1861 ggacgcgtcg ctcgcgatcg tgcctaacgt cggccacgtc ccgcaggtcg aaaacccgaa
     1921 ccgcacggca acactaatca ctcgcttcac aagatcactg ggctaagcgc gctcgttcta
     1981 gatcaaaagt ctggaaacga gaaactggaa acgagaaacc ggccaagggc cactggcgtt
     2041 ttggaggaaa aggcgcgaca atagcgccca taatcccccg aatacattcg aatctgcgta
     2101 tcagggtggg tggacgctat gggtgcctcc ggtttcgggc aatctcggtg gagatcgcgg
     2161 ttcctgctgt tcgggctgct gttccttttg ctgtcgccgg tcgcgttcgt ggccgcgcgc
     2221 tctttcgtta cactgaccga tccatcgccg gcggtcggtc gcgcccaggt cgtgacgcag
     2281 ggcatctcgg agctgccggg taccgagtac gtgatgcggg tcgtgcgccg gaccgcgccc
     2341 gcgctcggcg atgccaaggt cggccggcgg gtgctcggct tcgccctcgc caccgacgaa
     2401 ccgatcctaa tcaccgatgt cgatggcgac ggaacgttca aggatcgtgc acggctcgcg
     2461 ccgggtgagg cgtatatgac cctcgacggc caacgccaga ttcgcgccag cctgagcggc
     2521 aagccgacgc aataccttag tgtcgagttc gtcccgccgg accgcagcag cgacatcggc
     2581 acgggcgaat tgctcttcgt cagcgacacg atcactactc cgcccggcca gcgggacgtc
     2641 gacctggtgc gaaacgtcct tgcccagcag gacatcgcga aggtgccgga caccggcggc
     2701 cagatcatgg tgctcgccac cgacggcgcg atcgacatcc tgccgacgcg gacacgcatg
     2761 aggcgtctcg agtccggtca gagcgcagtc ttcaacgcgg aggagatcga gattcggccg
     2821 tccgagactt cggcgtatgg tgtcccgacc aaccagctcg gcgcgctgac cgacatgctg
     2881 cagaccgaca acccggtcgc cgggtacgtg gtagtcgtga tcgggccgga agtgccgaag
     2941 accggcgagc cgaccccgtc accgacacaa cctgcggcat ccgccacaat tgcggcgccc
     3001 acgatcaccg ctgtggctga gcgcggttcg atcggcgtga ccggccggct ttgcaatccc
     3061 ggcgtcacga tcgagacgat cagcgacgac aactgcccgg tcatcccgaa tggctacgac
     3121 ctggccctga gcggagccac gctcaatcgc actctcgccg acgcgtcgca ggtcgattcg
     3181 agctggtact ggtcggacct gccgcttggc acatatgcgc tgaccgcgac cggctatcct
     3241 ggccgcgcca ccgactactt catcccgggg tccgcggcgg tcggtggatc atccgcatcc
     3301 ggctacacgg tcacgctgga ccagtccagt ccaaacatcg tcctgccggt ttacttcctg
     3361 cagggccgcg cgcagccgac atcatccgcc gtcaccttca cgatccaggc gtgccagcag
     3421 gggctggtca attgcgcctc accgagccag tacaacgtcg atccgcagcc gtacctcgtc
     3481 ggcccgaacg gccagacact gaccgcggcc gatgcacagc gatcaggtga tgcctacacg
     3541 tggaccctgc cggccggaac ctggtccttc taccaacaag gctggcagag ctactactat
     3601 gtcgatggcc agacaatcga tggcggcaag ccgtatgtct tcacgacgga cggctcaacc
     3661 gctgccagtc attatgtcca ggacgagatc atcgtcatcc agtaatcacg ccagcggcgg
     3721 tcgcgttcct tgccagggtc gagcctgctc gaacgcggcc gccgcgcgca gcaccagcgc
     3781 atcatccttg agccggccga caatctggag cccggccggc agcccgtcgc tcgcgaatcc
     3841 gcatgggact gtcgccgccg gcaggccgtt gaggttgaac gggtacgtga acgcggtcca
     3901 gctcagatag gacgtctccc ggccgttgat cgtggcggga tagttctcgc ctaccggaaa
     3961 cgccgggcat ggcagggtcg gcgtcaagag caggtcgtaa tcgcgcagcc agtcggccca
     4021 cgccgccgcg tagataccct gatcggtccg tgcccgcgcc acatcggccg ccgtgaactt
     4081 ctggccctgc tcgaccagat cggccagccc gggatccatg atgtcgcgaa cctggtcgaa
     4141 gtcgtcgcga cggcccccag ccattgccgc cacccagagc acatgctcga tcggccacgg
     4201 atcggggaga ttcggatgat cgtcgacaac ctccgcgccg agatcagcga agaccttggc
     4261 cgcattctca gcgagcgccg cgatctctgg ctcgatcgcc gcgtagccga gatcctgact
     4321 ccaggcgaca cgcagacctc gcaagtccag gtccgcgagt gcatccacgt agtcgacgtt
     4381 ggaatcccac gaggtccggt cgcgcgggtc ggcaccggcc acgacgttca gcatcagcgc
     4441 cgcatcgcgc acccaccaag tcatcgggcc gtgataggcg agatcggcaa ccccgctcgc
     4501 cgggaacacc gggaccagcc cgatgctcgg cttcagcccg aagatgccgc taaatccgga
     4561 cgggatccgg atggatccgg cgccgtcggt cccgtgcgcc atcgtgccga ttccagccgc
     4621 gatgcacgcg cccgctccgc cgctggatcc gcccggcgtt cgctcgaggt tccaggggtt
     4681 gcgcgtcgtg ccgctgaccg ggctggttgt ctcgcccttc cagcccagtt ccggcgtcgt
     4741 cgtctggccc agcaagaccg ctccagcatc gagcacgcgc tggacgaaag gcgagctgaa
     4801 cgaggccggt gcgtcgtcgg cgtagatctt cgagccgcgc cggcagcgca acccctcgat
     4861 gaatgtgatg tctttgatcg tgaccggcac gcccgacagc ggcccggccg cccccgcccg
     4921 gtagtcggcc tcggccttct tggccgccgc ccgggcaata tcgccggtga aatcggtaaa
     4981 cgcgttgagc tgcggattga gccggtcaat tcgagccagc atcgcctccg acacctcaac
     5041 cggcgaaatc gccccactcg agtacccgcc gatcaactcc cccgccgaca aatacgcaaa
     5101 atcgtcccca gccactttta gcccgctccc gatgatccac aactaactga gcggcatcgt
     5161 acccgacagc agaaagtctg attgtggcta cggtccgaat tttggaggct cgatgaacaa
     5221 cacgggttcg tcgaagttgg ttagtacgac ggtattttcc ccgaccgaga tctgccctgc
     5281 tgggtgaacc gtagcggcgg tataagtcc
//
LOCUS       DATAOZ010000289         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2750037, whole genome shotgun sequence.
ACCESSION   DATAOZ010000289 DATAOZ010000000
VERSION     DATAOZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2750037"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..572)
                     /locus_tag="VHR64_07610"
     CDS             complement(36..572)
                     /locus_tag="VHR64_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302732.1"
                     /translation="MSFNVKKRGKLTYYYTGDRPVLSALVTEEQSDGDLKIHFAGLTG
                     GYSAKSVLGLEELVTMDPHREIPLVFQCWEKWLRDAGIAQSLREVDFIEMHAFGCQPK
                     SPNPLVDPVGFAAEQDRLRAAYAEAYAGFFADNLPENGVPCRFTVHLVDVPDKAASYE
                     FYSTALLQRSLQSSNSTS"
     gene            complement(643..1755)
                     /locus_tag="VHR64_07615"
     CDS             complement(643..1755)
                     /locus_tag="VHR64_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3302733.1"
                     /translation="MDTLYKDLGLKPVINASATLTRLGGSRMPQEVLQAMTAAAESFI
                     DLNALQERVGARIAELTNNEACYVSSGAAAGIMLSVASCIAGTDPAHTFVFPHLDGLP
                     KTEVIVQKVQRNGYDYAIAQTGATVVEIGNTAEELEAAISDKTACIVWFAGGPLAKDA
                     LPVEEVIRIAKQRNVPVIVDAAAQIPPVESLWRYTRDLGATGVVFSGGKGIRGPQSSG
                     LVLGTKELIAGCRANGNPNHSLGRPMKVGKEELAGILAAVEYTLEQDETALLEQYENI
                     VHGWIEDLSGLPGVTAERRFPSEAGQPHGRAFIHFNAPCPLSRDEIVKRLWDGDPCIA
                     VTAVQDEPDAIALNPQTIDPAEADIIVQRIRQLVVG"
     gene            1880..2587
                     /locus_tag="VHR64_07620"
     CDS             1880..2587
                     /locus_tag="VHR64_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302734.1"
                     /translation="MPFLIEDTGKPVGGVVDAGRDASNDVIGGAHHWRLRWPGLIALV
                     VAAGIYMILPGSLVVGPDWALPVALAVLVVGHEIARRGGRHELSRKIGIWIAALLTAG
                     VTASVVLLVARLPGKGTAPSSLLRDAALIWSINVLAFATWYWEIDGGGPVCRARGGYR
                     STDLVFPQMSDGPAASHGWSPAYLDYLFLAFNTSTAFSPTDTLALSHRVKVLMMAQSL
                     ISVVIVGALIARAINTL"
     gene            complement(2671..>2955)
                     /locus_tag="VHR64_07625"
     CDS             complement(2671..>2955)
                     /locus_tag="VHR64_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3302735.1"
                     /translation="TARIITGAALIMVIVFGGFATGELVAFQQMGFGMAVAVFLDATV
                     VRTILVPAAMALLGDRNWYLPKWLSWLPNVSVEGAPVLAPAPALTASAAD"
BASE COUNT          473 a          902 c         1043 g          537 t
ORIGIN      
        1 gtcaagtcaa tatgcgcgcc ccggagccgg ttccgctaag acgtgctgtt gctgctctgc
       61 aacgagcgct ggaggagcgc ggttgagtag aactcgtagg aggcggcctt gtccggcacg
      121 tcgacgaggt ggacggtgaa gcggcagggg acgccgttct cgggcaggtt gtccgcgaag
      181 aagccggcgt aggcctcggc gtaggcggct ctcaggcgat cctgctcggc cgcaaagccg
      241 accggatcga caagcggatt tggactcttc ggctggcagc cgaaggcgtg catctcgatg
      301 aaatcgacct cgcgcaggga ctgggcgatg ccggcgtccc gcagccactt ctcccagcac
      361 tggaagacga gcgggatctc gcggtgcgga tccatcgtaa ccagctcctc gagcccaagc
      421 acgctcttgg ccgagtagcc gccggtcagc ccggcgaagt ggatcttgag gtcgccgtcc
      481 gattgctcct cggtgaccag cgccgagagc accggccgat ccccggtgta gtagtaggtc
      541 agcttgccgc gctttttgac gttgaaggac atgaagtctc ctttcgggtt gatggtggtt
      601 ggttgatggt tgatggtgga tgggacgaag aaccaaccac catcaaccaa ccaccagttg
      661 ccggattcgc tgcacgatga tgtccgcttc ggcggggtcg atggtttgcg ggttgagggc
      721 gatggcgtcc ggctcgtcct gaactgcggt gaccgcgatg caggggtcgc cgtcccagag
      781 gcgcttgacg atctcgtcgc ggctgagcgg gcagggcgcg ttgaagtgga tgaaggcgcg
      841 gccgtgcggc tgcccggcct cgctcgggaa gcggcgctca gccgtgacgc cgggcagacc
      901 ggacagatcc tcaatccagc cgtggacgat gttctcgtac tgctcgagga gggcggtctc
      961 gtcctgctcg agggtgtact cgacggcggc gaggatgccg gccagctcct ccttgccgac
     1021 cttcatcggg cggccgagac tgtggttcgg gttgccgttg gcgcggcagc cggcgatcag
     1081 ctccttcgtg ccgagcacga gaccgctcga ctgcggacca cggatgccct tgccaccgct
     1141 gaagacgacg ccggtcgcgc cgaggtcgcg ggtgtagcgc cagaggcttt cgactggcgg
     1201 gatctgggca gcggcgtcga cgatgaccgg gacatttcgc tgtttcgcga tccggatcac
     1261 ctcctcgacc ggcagcgcgt ctttggccag cggtccgccg gcgaaccaga cgatgcaggc
     1321 ggtcttgtcg gagatggcgg cctcgagctc ctcggcggtg ttgccgatct cgacgaccgt
     1381 ggcgccggtc tgggcgatcg cgtagtcgta gccgttgcgc tggaccttct ggacgatgac
     1441 ctcggtcttc ggcaggccgt cgaggtgcgg gaagacgaag gtgtgggcgg ggtcggtccc
     1501 ggcgatgcag gaggcgaccg agagcatgat cccggcggcc gccccggacg agacataaca
     1561 ggcctcgtta ttggtgagct cggcgatgcg cgcgccgacc cgttcctgca gggcgttgag
     1621 atcgatgaac gattcggcgg cggccgtcat ggcctgcagc acttcctgag gcatccgtga
     1681 tccgccgagg cgggtcaggg ttgccgaggc gttgatgacg ggcttgaggc cgagatcctt
     1741 ataaagcgtg tccacatttc ctcctgtccc gggattggcg ggactccgtt cgtcagccta
     1801 ccgaaaatcg ccagcggatg tgcgaatggg tgccggacgg cacaccgtcg ccagagtgga
     1861 cactatgatt gggtgacgat tgccgtttct aatcgaagac accgggaaac cggtcggggg
     1921 cgtggtggac gcgggacgag atgcatcaaa cgatgtgatc ggcggcgccc atcactggag
     1981 attgcgctgg cccgggttga tcgcgctcgt ggtggctgcc ggcatctaca tgatattgcc
     2041 cggaagcctc gtcgtcggac ccgattgggc gctcccggtt gcgttggccg tgctggtggt
     2101 cgggcacgag atcgcgcgac gcggcgggcg acacgagctg tcccgcaaga tcggtatctg
     2161 gatcgccgca ctgttgaccg ccggggtcac ggccagcgtg gtcttgctgg tcgcgcgttt
     2221 gccaggaaag gggaccgctc catccagcct gcttcgggac gccgcgctga tctggtccat
     2281 caacgtgctc gccttcgcca cgtggtactg ggagatcgat ggcggtggac cagtctgtcg
     2341 cgccaggggt ggctatcgca gcacggacct cgtgttcccg cagatgtcgg atggtccggc
     2401 ggcgagccac ggctggtcgc cggcgtacct ggactacctc ttcctcgcct tcaatacaag
     2461 caccgcgttc agtcccaccg atacgctggc gctgtcgcac cgcgtcaagg tgctgatgat
     2521 ggcccaatcg ctgatttccg tcgtgatcgt cggagccctg attgcccgtg cgatcaacac
     2581 cctctgagcg aatgtctcga gttccgggca aaccaacgtg ccggacgggt ttccgtccgg
     2641 cacgttggtg agagaggagt gggtggcgtg ttagtcggcg gctgaggcgg tcagggccgg
     2701 agcgggggcg agcaccgggg cgccctccac gctcacgttc ggcagccagc tcagccactt
     2761 cggcaggtac cagttgcgat caccgagcag ggccatcgcc gccggcacca ggatcgtccg
     2821 gaccaccgtc gcatccagga agaccgcgac cgccatcccg aagcccatct gctggaaggc
     2881 gaccagctcg cccgtcgcga agccaccgaa cacgatcacc atgatcaggg cggcgccggt
     2941 gatgatccgc gcggt
//
LOCUS       DATAOZ010000290         6846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3274997, whole genome shotgun sequence.
ACCESSION   DATAOZ010000290 DATAOZ010000000
VERSION     DATAOZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3274997"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VHR64_07630"
     CDS             <1..657
                     /locus_tag="VHR64_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302736.1"
                     /translation="GDHPADRGRVVWLRRRCSRWFPATLLVLGIVLAVGIMTADNELI
                     THDRLMDDIGMNLSALKHFRFWAIPAATFVQASPGVAWTMLLLVGLPMIALEFLAGSW
                     RALVTFLLCDWLTAPVTVVILWILSNLGDANAGRFLMDPDTGSSAAAHGVIAAAIMML
                     PAKLAAIGMTILVGITIIALSFFRLDTSLAHFLGIVVGAVFGAIWKRQIRREPAYHVT
                     "
     gene            688..1545
                     /locus_tag="VHR64_07635"
     CDS             688..1545
                     /locus_tag="VHR64_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3302737.1"
                     /translation="MTQRLSFGVVIEQQHWDWVSNVELWKQAEELGFESIWINDHFYS
                     LGDSIDREGFEASTTLAALAMTTTKPKIGVLTYGNAHRNPAILFKEVVTVDHMSGGRV
                     IFGIGAGWNEPEHRAFGIPFPGAGDRVAMLEEALELFDELESNDRTTFHGRYYQLEDA
                     PFVPKPVNGHIPRLIGGRRPKMLRVIAKHADIWDSGLEADEYTVALRTIREHAAEFGR
                     DPDAIVASTPVWRQKMSEKEFADRVRAFYKAGVRQMLIQHPADRVGIDMIPGLVERVI
                     PELRAELEG"
     gene            1555..2412
                     /locus_tag="VHR64_07640"
     CDS             1555..2412
                     /locus_tag="VHR64_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3302738.1"
                     /translation="MGQKLTFGVVIRPQHWPWADQISEWPKAESIGFESIWLNDHFHS
                     LGHTIDNPAFEASTTLAAVALSTKTAKIGVLTYGNTHRNPAILFKQIVTVDQMSGSRV
                     IFGIGTGWHESEHAAYGVTLPSAGDRVRMLEETLAMFRQSETNDRTTFHGRYYSLNDA
                     PFWPKPVNGRIPILIGGTKPKMLRVIGRNADIWDSSMAVDDYAAALTTIRGHAREAGR
                     DPEAVMGSANVWEGEISDKEFADRVRAYHKAGARQMLLKYTPDRTGVETFPRLMTEIV
                     PELRAELDR"
     gene            complement(2475..3470)
                     /locus_tag="VHR64_07645"
     CDS             complement(2475..3470)
                     /locus_tag="VHR64_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3302739.1"
                     /translation="MTQTMDLLQPVPSRPNGHFPSTRARSLTPIRKRSSSHQWIAALL
                     TAALILLTFAAAFEAIHSGEGNPNDGAKIPAGFAPATPSPSSSPTTEGLFDVGGHKLY
                     IKCTGTGSPTLVFLNRLDETNLYWGDIPSSFAQDTRVCVYDRANQGRSGHAPMGTASD
                     SVADLHSLLTAARVPGPYLLVGDEFGGLVSTMYAGTYPDDVAGLVLIGPLHPWQAEVE
                     SLIPSAYRTGLRDEHASNREQVAYYQSLDQELALLPKVGDINVVVLVGDNIAIPAEWP
                     ADLITPKIYEDYQRFADAFSQGKMIRLHSTAYIVQTVPDQVITELRKVLTGLQKP"
     gene            complement(3688..4299)
                     /locus_tag="VHR64_07650"
     CDS             complement(3688..4299)
                     /locus_tag="VHR64_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3302740.1"
                     /translation="MRSTAPHEVLEDRSASAIPDALLVLDAQDDRAAFGALYDRYFDD
                     IFRFCYYRLGNWQEAEDAAGDIFANALAGLSRYRSGDLPGSFRSWLFAIARNGVSDRH
                     RISFRHPSDSLDAAAEILDGAPTPEEAAIESDNHEYMLRLLAQLKPAQRDLLQLRLAG
                     LRNAEIARILGRSHDAVRKEQSRIVQTLRHTLECDPDQEVPHG"
     gene            4636..5061
                     /locus_tag="VHR64_07655"
     CDS             4636..5061
                     /locus_tag="VHR64_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3302741.1"
                     /translation="MPFAEAVADFPEGKINLRPRNVPYSFWHLLEHLRLTQLDIVEYV
                     TSADYQEKEWPRDYWPAPDAEATKQQWIETIALFNRDLEQLIGIVSDEATDLFASVPS
                     NPEHDILREMLIVADHNAYHTGELGILRQVEGAWGPAHR"
     gene            5253..6038
                     /locus_tag="VHR64_07660"
     CDS             5253..6038
                     /locus_tag="VHR64_07660"
                     /inference="COORDINATES: protein motif:HMM:NF009093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX3302742.1"
                     /translation="MTESLADRTAIVTGAGSGIGAAIASELARAGAHVVVQDLRLDAA
                     RSTVEAIRSAGGSADASEGDVTQPDQIRRTVDETVASRGRIDILVNNAGLQFVSPIDD
                     YPLEKWNQLIAVLLTGPFLFIQAVLPAMRAQKHGRIINISSINGKRGEPGKAAYCSAK
                     HGVIGLTRTAAIETARDGITVNAICPGLVMTPLIEGQIADLSRVHNMPPDEVIERHFL
                     AKVPTGRATETTEIGSLACYLASDEAASITGQAINVDGGMIMY"
     gene            6217..6693
                     /locus_tag="VHR64_07665"
     CDS             6217..6693
                     /locus_tag="VHR64_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302743.1"
                     /translation="MHRHYLGSIQADAWPVIAHDQRASLEQIIVESRHPAGNGQIHLS
                     PQQALYVQSRLALIAIGCLHHEDQHFAGVVTDHAPNPAEHTMRECGARVWPVGLVTAS
                     QPPAVDEVIVESRHPDGDGEIHLSPDQADFVAKQLTHIALQYLHDDVWGGEDARQQ"
BASE COUNT         1269 a         1981 c         2342 g         1254 t
ORIGIN      
        1 ggcgatcatc cagctgaccg agggcgggtc gtctggctga ggcgccgatg cagccgctgg
       61 ttcccggcga cgttgctcgt gctcgggatc gtcctggccg tcgggatcat gaccgcggac
      121 aacgagctga tcacgcatga ccggctgatg gacgacatcg gcatgaactt gtcggccctc
      181 aagcacttcc ggttctgggc catcccggcc gcgacgttcg tgcaggcgtc gcctggagtg
      241 gcctggacga tgttgctgct ggtcgggctt ccgatgatcg cgctggagtt cctggccggg
      301 agctggcgcg cgctggtcac cttcctgctc tgcgattggc tgaccgcgcc ggtaaccgta
      361 gtcatccttt ggattctttc caatcttggc gatgcgaacg cgggccggtt tctgatggac
      421 ccggataccg gctcatcggc ggccgcgcac ggggtgatcg ccgcggcgat catgatgctc
      481 ccggcgaagc tggccgcgat cgggatgacg atcctggtcg ggatcacgat catcgcgctc
      541 tccttcttcc ggctcgacac gtcgctcgcg catttcctgg ggatcgtggt cggggcggtc
      601 ttcggcgcga tctggaagcg gcaaatccgg cgcgaacccg cgtaccatgt cacctgactg
      661 gattcgggcg agtggctgga ggcgcaggtg acgcagcgat tgagcttcgg cgtagtgatc
      721 gagcagcagc actgggactg ggtatcgaac gtcgagctgt ggaagcaggc cgaggagctg
      781 ggcttcgaat cgatctggat caacgaccac ttctattcgc tgggggacag catcgaccgc
      841 gagggattcg aggcctcgac gacgctggcc gcgctggcga tgacgaccac caagccgaag
      901 atcggcgtcc tgacgtacgg caacgcgcat cgcaaccccg ccatcctgtt caaggaggtg
      961 gtgacggtcg accacatgag tggcggccgg gtcatcttcg ggatcggcgc gggctggaat
     1021 gagccggaac accgcgcctt cggtatcccc ttcccgggcg cgggggaccg ggtcgccatg
     1081 ctcgaggagg cgctcgagct gttcgacgag ctcgagtcca atgaccgcac gaccttccat
     1141 ggccggtact accagctcga ggacgcgcca ttcgtgccga agccggtcaa cgggcacatt
     1201 ccgcgactga tcggcggcag gaggccgaag atgctgcgcg tgatcgcgaa gcacgccgac
     1261 atctgggaca gcggtttgga ggcggatgag tacaccgttg cgctgcggac gatccgggaa
     1321 cacgcggcgg agttcgggcg ggatcccgat gcgatcgtgg cgtcgacacc ggtctggcgt
     1381 cagaagatgt cggagaagga gttcgccgac cgggtgcggg ccttctacaa ggcgggcgtg
     1441 cgccagatgc tgatccagca tccggcggac cgggttggca tcgacatgat tcccgggctg
     1501 gtggagcggg tgatccccga gctgcgggct gagcttgagg gataggggaa cggcgtgggg
     1561 cagaaactga cgttcggggt tgtgatccgg ccccagcact ggccgtgggc ggaccagatt
     1621 tcggaatggc cgaaggcgga gtcgattggc ttcgagtcga tctggctgaa cgaccatttt
     1681 cactcgctcg ggcacacgat cgacaacccg gcgtttgagg catcgaccac gctggccgcg
     1741 gtcgcgctgt cgacgaagac ggccaagatc ggcgtgctga cctatggcaa cacgcaccgg
     1801 aacccggcga tcctgttcaa gcagatcgtg acggtcgatc agatgagcgg cagccgggtg
     1861 atcttcggga tcgggaccgg gtggcacgaa tcggagcacg cggcgtacgg ggtgacgttg
     1921 ccatcagccg gggaccgggt gcggatgctc gaggagacgc tggcgatgtt ccggcaatcc
     1981 gagacgaacg accgcacgac gtttcacggc cggtattact cgctcaatga tgcgcccttc
     2041 tggccgaagc cggtcaacgg gcggatcccg atcctgatcg gcgggaccaa gccgaagatg
     2101 ctgcgggtga tcgggcggaa cgccgacatc tgggactcga gcatggcggt cgacgactac
     2161 gcggcggcgc tgaccacgat ccgaggtcat gcgcgggagg ccgggcggga tccggaggcg
     2221 gtcatgggct cagcgaacgt ctgggagggg gagatcagcg acaaggagtt cgcggatcgg
     2281 gtccgggcgt atcacaaggc cggtgcgcgc cagatgctac tcaagtacac accggaccgc
     2341 acgggcgtcg agaccttccc gcgcctgatg accgaaatcg tcccggagtt acgggcggag
     2401 ctggaccgct gaaacggcag agagaccctg gatttctccg ggtctctcta ttcgactcgt
     2461 gtcgccgcag caagttacgg tttctgcaac ccggtgagga cctttcgtaa ttcggtgatc
     2521 acctgatccg gcaccgtctg gacgatatag gcagtggagt gcaggcggat catcttacct
     2581 tgcgaaaagg catcggcgaa gcgctggtaa tcctcataga ttttgggtgt gatcagatcg
     2641 gccggccatt ctgcggggat ggcgatgtta tcgccgacga gcacgaccac gttgatatcg
     2701 ccaaccttgg gcaacagggc cagttcctga tcgagggatt ggtaataggc aacttgctcc
     2761 cgattgcttg catgctcgtc cctgagtcct gtccggtacg cagacgggat cagtgactcc
     2821 acctcagctt gccacggatg gagcgggccg atcagcacga gaccggcaac gtcgtccggg
     2881 taggttcctg cgtacatggt ggagacgagg ccaccgaact catcgcccac cagcaggtag
     2941 ggcccgggca cccttgcagc cgtcaagaga ctgtggagat ccgccacact atctgaggcc
     3001 gtgcccatgg gcgcatgccc gctcctccct tggttggccc gatcgtagac gcagacgcga
     3061 gtgtcttgcg cgaaggagct cgggatatcg ccccagtaca gatttgtctc gtcaagtcgg
     3121 ttgagaaaga cgagggtggg tgaaccggtc ccggtgcact tgatgtagag cttgtgtccc
     3181 ccgacgtcga acaggccctc agtcgtcggc gaggagctcg gagacggagt tgcgggcgcg
     3241 aatcccgccg gaatctttgc gccgtcattt gggttgcctt ccccggaatg gatggcttcg
     3301 aacgcggctg caaaagtgag caggatcaat gccgccgtaa ggagagcggc aatccactgg
     3361 tggctcgatg aacgcttgcg aatcggtgtc aggctgcgcg cccgggtaga tgggaaatgt
     3421 ccatttgggc gacttggtac tggctgaagg aggtccattg tctgtgtcat cggtgactcc
     3481 tgttgctgtg gtgaattgct ggtaggtagg tttagtcgga gtcggactcg ctcgcgcgcg
     3541 gcggaggttg gaggtggcgt agcggcaagc gcttgcatgc gccggacgag cgctgcggcc
     3601 cggggatcga gcttccctgg gttggcgtcg tctgagacga ccaggtcgtc ccaaaatcga
     3661 tccaggaact ggccgtcacg atcgagctca accatgcggc acctcctggt ctggatcgca
     3721 ctcaagcgtg tggcggagcg tctgaacaat ccgcgattgc tctttgcgga ccgcgtcgtg
     3781 gctccggccg agaatgcggg cgatctccgc atttctgaga ccggccaagc gcagctgcag
     3841 cagatcgcgc tgggctggct tgagctgagc gagcagacgg agcatgtact cgtgattgtc
     3901 ggactctatg gccgcctcct ctggagtcgg cgcgccatcg agaatttccg cggccgcatc
     3961 cagtgagtcg gacgggtgac ggaagctgat ccgatggcga tctgagaccc cgttgcgggc
     4021 gatggcaaag agccagcttc ggaaactccc ggggaggtcg ccggagcgat aacggctgag
     4081 gccggcgagc gcattggcaa agatatcgcc ggcggcatcc tccgcctctt gccagttacc
     4141 gaggcggtaa tagcagaagc ggaaaatgtc atcgaagtag cgatcataga gcgccccgaa
     4201 ggccgcgcga tcgtcttggg cgtcaaggac gagcagcgcg tcagggatgg ccgaagccga
     4261 ccgatcctcc aagacttcat gaggtgctgt gctcctcatc cgcggctctc ctgcgtgatg
     4321 acgctcgtgt tcaacagggg taacgtgact ggtgaacttt tgtgaccgca cggctgcgaa
     4381 cggggcaggg ggtgaagctg gcaagcggat ctcaggtccg ttgtccagat taggcggccc
     4441 aggaggagtg cgaccggtca ctccggaggt cagatgatga gtgattagtg ttaggtatca
     4501 gcgtacctac ctggtcaatc ggaggtcggg accagaatcg gttggtggtc tgtatgaaga
     4561 aaggagcacg ttttgacggc gagagatctg ctgcgagcac aattagtggc gttgttgcgg
     4621 ggtgggaatg cgcatatgcc gtttgcggag gcggtggcgg attttccgga ggggaagatc
     4681 aatctgcggc cgcggaatgt gccgtactcc ttctggcacc tgcttgagca tttgcggctg
     4741 acccagctcg acatcgtcga gtatgtgacg agcgcggatt accaggagaa ggagtggccg
     4801 cgggactatt ggccggcgcc agatgccgag gcgacgaagc aacagtggat cgagacgatc
     4861 gcgctgttca atcgtgacct ggagcaattg atcggcattg tgtccgatga agcgaccgat
     4921 ctgttcgcct ccgtgccgag taatcccgaa cacgacatcc tgcgcgagat gctgatcgtg
     4981 gccgatcaca atgcctacca caccggcgag ctgggcatcc tgcgccaggt cgagggcgcg
     5041 tggggtccgg cgcaccggta gaccaggtac gtagcgcggc ccgcctctgg gccgcttgga
     5101 tatcgaccgc ggcttgttgc catgccagtc cggcttcacg gcccgtgcgt gccgtcagcg
     5161 taaggccggc cacggggggc cggcgctaca gttcatgcat cgagtcaacc gatgtgcggc
     5221 tcttcaatct caaagaccaa ggagaatgtc tgatgaccga aagccttgcc gatcggaccg
     5281 caatcgtgac cggcgccggg agtgggatcg gcgcggcgat cgcgtcggag ttggcccgcg
     5341 cgggagcgca cgtcgtcgtc caggacctta ggctcgatgc ggccaggtcc acagtcgagg
     5401 caatccggag cgcaggcggc tcggcggatg catccgaggg cgatgtcacg cagcccgacc
     5461 agatccggcg cacggtggac gagaccgtgg caagccgggg ccggatcgac atcctggtca
     5521 acaacgcggg attgcaattc gtcagcccga tcgacgacta cccgctggag aagtggaatc
     5581 agctcatcgc cgtgctgctg accgggccgt tcctcttcat ccaggcggtg ctgccggcga
     5641 tgcgtgccca gaagcatggc cgcatcatca atatctcgtc gatcaacggc aagcggggcg
     5701 agccggggaa ggcggcctat tgctcagcca agcacggtgt gatcggactg acgcgaacgg
     5761 cggcgataga gacggcgcgg gacggcatta cggtgaatgc gatctgccca gggttggtca
     5821 tgacgccttt gatcgagggc cagatcgccg atctctccag ggtgcacaac atgccgccgg
     5881 acgaggtgat cgagcgccat tttctcgcga aggtcccgac cggccgggcc acggagacga
     5941 ccgagatcgg gtcgctcgcg tgctacttgg cgtccgacga ggcggccagc atcaccggcc
     6001 aggccatcaa cgtggacggt ggcatgatca tgtactgagc cggcatcgta acccggcttg
     6061 atgttgcgaa actgatacac ccttcaggct cgtgttgcgc atcattgatc agtccggtga
     6121 tagcgtggca atgaccgctc gcctgagcgg ctgcggtgcc ggagccgatg gtcggtcgta
     6181 cacgccagga caccgctcga gattgggagg ggcgccatgc accgccacta tctcggatca
     6241 atccaggccg atgcgtggcc agtcatcgcg catgaccaac gtgcatccct ggagcagatc
     6301 atcgtcgaga gtcgacatcc ggccggcaac ggccaaatcc acctctcgcc ccagcaagca
     6361 ctctacgtcc agagtcgtct cgccctcatt gcgatcgggt gtcttcatca tgaagaccag
     6421 cactttgccg gagtcgtgac ggatcacgcg ccgaatccgg cggaacacac gatgcgggaa
     6481 tgcggagcgc gggtctggcc ggttggcctt gtcacggcca gtcagccgcc ggcggttgac
     6541 gaagtgatcg tcgagagtcg gcatccagac ggcgacgggg agatccacct gtcccccgat
     6601 caggctgact tcgtggcgaa gcaactgacc catattgccc tgcaatatct gcatgacgac
     6661 gtttggggcg gcgaagacgc gcgacagcaa tagcccctgg gtatccggga caagttatgg
     6721 tgcggttcta ccgcaccata acttgtcccg gatacccagg ggctattgct gtcgcgcgtc
     6781 ttcgccgccc caaacgtcgt catgcagata ttgcagggca atatgggtca gttgcttcgc
     6841 cacgaa
//
LOCUS       DATAOZ010000291         4989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_122687, whole genome shotgun sequence.
ACCESSION   DATAOZ010000291 DATAOZ010000000
VERSION     DATAOZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122687"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VHR64_07670"
     CDS             <1..514
                     /locus_tag="VHR64_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3302744.1"
                     /translation="PLGAAMARKEIANTIDEGSVLNHGITFGGHAVACAAGIANLDIM
                     LREDLPGKAKETGQYLRTQLEERLGNHPNVGDIRGAGLFLGVEMVKDRATKETLPYDA
                     DLLSGMTDEMRRRGLIIRNDSRGDPTTQLCPPLVITHEECDTVVDVLEQCFNQLGQAL
                     GTVGTTFAAG"
     gene            complement(580..2643)
                     /gene="ftsH"
                     /locus_tag="VHR64_07675"
     CDS             complement(580..2643)
                     /gene="ftsH"
                     /locus_tag="VHR64_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX3302745.1"
                     /translation="MQDPNKNRLPRNPFSGGGDKNDKNEKGTGPRRPKVPTWFVGLLL
                     VCIAVWYAYQFMSPSSDQSRVTVPYSAAVEQISAGNTAKAVIGTSNLELDLKSAVRWN
                     KDDEVFVANDAANASDYPQTSKLKAKIPPLIRQDNQELITLLEQQNVTYEGKDESGSI
                     LTSLLINFLPIIFIIALIVFMSRQMTRGQQNVFGFGRSRARLNDPERPQVTFADVAGE
                     EEAKKELTEVVDFLRNPAKYHQLGARLPRGVLLVGPPGTGKTLTARAVAGEAGVPFFS
                     ISASEFVEMFVGVGASRVRDLFDRAKTMHPSIIFIDELDAVGRQRFAGLGGSNDEREQ
                     TLNQLLVEMDGFDTDQEVIVMAATNRPDVLDPALLRPGRFDRQVTVGLPDRKGREAIL
                     GIHTRGIPVAKEVDLPGIARATTGFSGADLSNLVNEAALMAARKNRKEILNSDFDEAL
                     DKILLGTTRTNLMSPEERKVVAYHEGGHALVAHLTPGADPLRKVTIVPRGRALGVAIQ
                     TPEEDRYNVSRLYLEGRLSVALGGRAAEIATFDEVTTGAESDLKQATQLARRMVGLWG
                     MSDAVGPVYLGTGEEHVFLGREIVQDKAFSDATSERVDTAVRQIIEEALETAQTLIGA
                     NKPKLEKLVTALLEKETLDAVEVTEILGPRPQLSPREAEVMVPPPVAEPPLIGEPIST
                     GTRQD"
     gene            complement(2845..3195)
                     /locus_tag="VHR64_07680"
     CDS             complement(2845..3195)
                     /locus_tag="VHR64_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302746.1"
                     /translation="MRDSLAHGDIIQPDRVDELMDFLAPFIQTLLTQVEPGEFTTPMF
                     IEFMRSIPEGDALYEEAIRRWGEQDEHMSRMVIHGQVIPGILRRSSGVEWAGFAHGEP
                     DPYGVPAWWRIRES"
     gene            3289..4536
                     /locus_tag="VHR64_07685"
     CDS             3289..4536
                     /locus_tag="VHR64_07685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3302747.1"
                     /translation="MTTTTNGHKVNGTGQVLPKTADVVVIGAGVNGLATAFELTRRGA
                     GKVVILERKHVGAGATGKSGALVRQHYTNVPEGLLTLHSLDTFLNWGDVVGHGDPGFI
                     QPGFLRVVAPADEAKIRANVDNLHAAGINTSIVTPEEIAEIEPLLFTGDISVAAFEPD
                     AGYADPNATAYGFAQAATANGATLVLDCEATGIDLRGDRVVAVETNRGRIETRQVLIA
                     AGPWADRLLDPLGYAIGLTPYKSQVVIFRWPMAMDQGRKHRVVIDTVYESWLRPEGDA
                     GTLIGIEFPDRKGDPDSFPQFPPVDYIEQTRAALAARFPVFANSTMRGGWTGMYMMSP
                     DQHPIIGKMDQVEGLYVMTGDSGSCFKTAPATGVCLAELMTEGEAKLVDLTPFRPSRF
                     AEGKPWVDEHRYGHAGETLSISR"
     gene            4533..>4989
                     /locus_tag="VHR64_07690"
     CDS             4533..>4989
                     /locus_tag="VHR64_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3302748.1"
                     /translation="MSRGHTLYLLHCGTLIEPDGTGGTYPIQVPCYLIVAASGKRYLI
                     DTGNPAGLIGATNCQPWYPARCEINPADDPIARLAELDVTPNAIDAVIATHFDFDHAG
                     RYDAFGPLGTDVWVQRAHIASALMDADRYDPNLWNVPGLRWQQVDGDHEI"
BASE COUNT          884 a         1633 c         1586 g          886 t
ORIGIN      
        1 gccgcttggc gcggcgatgg cgcgcaagga aatcgccaac accatcgacg agggaagcgt
       61 cctcaatcac ggcattacgt tcggtggcca cgcggtcgcc tgcgcggccg gaatcgcaaa
      121 cctcgacatc atgctccgcg aagatctccc gggcaaggcg aaggagaccg gccaatatct
      181 ccgcacacag ctcgaagagc ggctcggcaa ccacccgaat gtcggcgaca ttcgcggcgc
      241 cggcctgttc ctcggcgtcg agatggtcaa ggaccgcgcg acgaaggaga ccctgcccta
      301 cgacgcggac ctgcttagtg gcatgaccga cgagatgcgg cggcgcggct tgatcatccg
      361 caacgacagc cggggcgacc cgaccacgca gctctgcccg ccgctggtga tcacgcatga
      421 ggagtgcgac accgtggtcg acgtcctcga gcagtgcttc aatcagctcg gccaggccct
      481 cggcacggtc ggcacaacct tcgccgccgg ctgatcatcg cgattcgaca cgaaaaagga
      541 cccgggagcc aatcggctcc cgggttcttc gcgtttgcgt tagtcctggc gggttccggt
      601 cgagattggc tcgccgatca gcggcggctc cgcaaccggc ggcgggacca ttacttcggc
      661 ctcgcgcggg ctgagctgcg gtcgcggccc aagaatctcg gtcacctcga cggcgtccag
      721 cgtctccttt tcgaggagcg cggtcaccag cttctccagc ttgggtttgt tcgccccaat
      781 cagcgtctgc gccgtctcca gcgcctcctc gatgatttgg cggaccgcgg tgtcgacgcg
      841 ctcggacgtc gcatccgaga aggccttgtc ctggacgatc tcacggccga ggaagacatg
      901 ctcctcaccc gtgccgagat agaccgggcc gacggcgtcg ctcatgcccc agagaccgac
      961 catccgccgg gcgagctgtg tcgcctgctt gaggtcgctt tccgcgccgg tcgtgacctc
     1021 atcgaaggtc gcaatctcgg cagctcggcc accgagggca acggacaacc ggccctcgag
     1081 gtagaggcgg gagacgttat agcggtcctc ttccggtgtc tggatcgcga cgcccagcgc
     1141 ccggccgcgc ggcacgatgg tcacctttcg gagtggatcg gcacctgggg tcaggtgcgc
     1201 gaccagcgcg tgtccacctt cgtggtaggc aacgaccttg cgctcctcag ggctcatcag
     1261 gttcgtccgg gtcgtgccga gcaggatctt gtcgagcgct tcgtcgaagt cggagttcag
     1321 gatctccttg cgattcttgc gtgccgccat cagcgcggcc tcattgacca ggttcgagag
     1381 gtcggcgccg gagaagccgg tcgtcgcccg ggcaataccc ggcagatcga cttccttggc
     1441 caccgggatg ccgcgcgtgt ggataccgag gatcgcctcg cgtcccttgc ggtccggcaa
     1501 accgaccgtg acctgccgat cgaatcggcc cggccgcaga agcgcgggat cgagcacgtc
     1561 cggccggttg gtggccgcca tcacgatcac ttcctgatcg gtgtcgaagc cgtccatctc
     1621 gacgaggagc tggttcaatg tctgctcgcg ctcgtcgttc gacccaccga gcccagcgaa
     1681 ccgctgccgg ccgacagcat cgagctcgtc gatgaagatg attgacgggt gcatcgtctt
     1741 ggcgcggtcg aagaggtcgc ggacgcgact cgcgccgacg ccgacgaaca tctcgacgaa
     1801 ttccgacgcg gagatgctga agaacggcac accggcctct ccggcgacag ctcgcgcggt
     1861 cagcgtcttg ccggtaccgg gaggtccgac caggagcacg ccgcgcggaa ggcgggcgcc
     1921 aagctggtgg tacttggccg ggttacgcag gaagtcgacg acttcggtga gctccttctt
     1981 cgcctcttcc tcaccggcca cgtcggcaaa cgtcacctgg ggccgctcgg gatcattgag
     2041 gcgagcgcgg ctccggccga agccgaagac attctgctgc ccgcgggtca tctggcggct
     2101 catgaagacg atcagggcaa tgatgaagat aatcggcagg aagttgatca ggaggctggt
     2161 caggatcgag ccactctcgt ccttgccctc ataggtcacg ttctgctgtt cgagcagggt
     2221 gatcagctcc tggttgtcct gccggatcag cggcggaatc ttcgccttca gcttcgacgt
     2281 ctgcggatag tcggacgcgt tggccgcatc gttggcgacg aagacctcat cgtccttgtt
     2341 ccagcggacg gccgacttga gatcgagctc gaggttactg gtgccaatca ccgccttggc
     2401 ggtattgccg gcgctgatct gctcgaccgc cgccgagtac ggcacggtta cgcggctctg
     2461 gtccgagctt gggctcatga actggtatgc gtaccagacc gcgatacaaa caagcagcaa
     2521 gccgacaaac caggtcggca ccttgggtcg ccgagggccg gtgccctttt cattcttgtc
     2581 gttcttgtct ccaccgcccg aaaacgggtt gcgcggcaga cgattcttgt tagggtcctg
     2641 catcaagtgt ccttacggtc gggcatggag accgtttcca gaaggtcggc ccagtgggac
     2701 tggccgcgtc gttcgttggt tgacggtgta tccgtgggga gtcgaccgta cagaatcaag
     2761 cgcaatatgt tgacctaatc ctaccgccga ttgccggcgg atttccatta tcgccggtcc
     2821 ggcaacaccc ggtcgaccag ggaatcagga ctcccgtatt cgccaccatg caggcacgcc
     2881 atatgggtcc ggctcgccat gcgcaaatcc cgcccattca acgcccgacg agcgccggag
     2941 gatgcccggg atcacttggc catgaatgac catccggctc atgtgctcat cctgctcgcc
     3001 ccatcgtcgg attgcctctt cgtagagtgc atccccctct ggtatcgaac gcatgaattc
     3061 gatgaacatc ggcgtcgtga actcgcccgg ctcgacctgc gtgaggagcg tctggataaa
     3121 gggagcgagg aagtccatca gctcatcgac ccgatcgggt tggatgatgt cgccgtgggc
     3181 cagtgaatcg cgcatgtcga ggttccgggt tccagtcacg aagcgggagt aatctgccgc
     3241 atacttcccg cgcggaaatg cggcattgcc tacgggagcg tatcaggtat gacgaccacc
     3301 acgaacggcc acaaagtgaa cggaaccgga caagttcttc cgaagacggc cgatgtcgtc
     3361 gtgatcggcg cgggagtcaa tggccttgcg actgcgttcg agctgacccg gcgaggcgcc
     3421 ggcaaggtcg tcatcctcga gcgcaagcac gtcggagccg gcgcgaccgg caaatccggc
     3481 gcgttggtcc gccagcatta cacgaacgtg cccgaaggcc tgctgacgct gcacagtctc
     3541 gacacgttcc tcaactgggg cgatgtcgtc ggacacggcg atcccggatt catccagccc
     3601 ggcttcctgc gggtcgtcgc gccggcggac gaggcgaaga tccgggccaa cgtcgacaac
     3661 ctgcacgccg ctggcatcaa tacctcgatc gtgacgccgg aggagatcgc cgagatcgag
     3721 ccgctcctct tcaccggcga catctcggtc gccgcctttg agccggacgc cggatacgcc
     3781 gatccgaacg cgaccgccta cggtttcgcc caggcggcaa ccgcgaacgg cgcgacgctc
     3841 gtgctcgact gcgaggccac tggcatcgat ctccggggcg accgcgtggt ggctgtcgag
     3901 accaatcgcg gccgaatcga aacccgccag gtgctgatcg ccgccggacc gtgggcggat
     3961 cgcctgctcg acccgctcgg gtatgcgatc ggtctgacgc cgtacaagtc gcaggtcgtc
     4021 atcttccgct ggcccatggc gatggaccag gggcggaagc accgggtcgt catcgacacc
     4081 gtctacgagt cgtggctgcg gccggaaggc gacgccggca cgctgatcgg catcgagttc
     4141 ccggaccgga agggcgatcc cgacagcttt ccgcagttcc cgccggtcga ttacatcgag
     4201 cagacccggg ccgcgctggc cgcccgtttc cccgtcttcg ccaactcaac gatgcgcggc
     4261 ggctggaccg ggatgtacat gatgagcccg gatcagcatc cgatcatcgg caagatggat
     4321 caggtcgagg ggctctacgt gatgaccggt gacagcggct cgtgcttcaa gacggcgccg
     4381 gccactggtg tctgcctcgc ggaattgatg accgaaggcg aggcaaaact ggtcgacctc
     4441 accccgtttc gccccagccg ctttgccgag ggcaagccct gggtggatga gcatcgatat
     4501 ggacacgccg gcgagacgct cagcatctcc cgatgagcag gggccacacg ctctacctgc
     4561 tgcactgcgg aacattgatc gaaccagatg ggacaggcgg cacctacccc atccaggtac
     4621 cgtgctatct gatcgtcgcg gccagcggca agcggtacct gatcgacacc ggaaatccgg
     4681 ccgggttgat cggcgccacc aattgccagc cctggtatcc cgcccgctgc gagatcaatc
     4741 cggccgacga tccgatcgcc cggttggccg agctcgatgt cacgccgaac gcgatcgatg
     4801 ccgtcatcgc tacgcacttc gatttcgatc atgccggccg gtatgacgcc ttcggaccgc
     4861 tcggaaccga cgtctgggtc cagcgcgcgc acatcgcttc cgctcttatg gatgcggatc
     4921 gttacgaccc gaacctctgg aacgtgcccg gacttcgctg gcagcaggtc gacggcgacc
     4981 acgagatcg
//
LOCUS       DATAOZ010000292         4602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8277223, whole genome shotgun sequence.
ACCESSION   DATAOZ010000292 DATAOZ010000000
VERSION     DATAOZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8277223"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VHR64_07695"
     CDS             <1..796
                     /locus_tag="VHR64_07695"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX3302749.1"
                     /translation="SVDPARQQFFQGARRELLQTYLRRDAEGLLNWVLTLYPTDAYAQ
                     DADMSTDEFTEFVFRAGKLHTDDPISAWKAETDFAQRIVNWLDGKQEIHLTGPGTDLH
                     VSVANRIWINSGDATKNFPDGEVFTGPVEDGVNGVVSFSFPVVTAGREIEGIVLRFEH
                     GKVAQATATRGQEYLEAVLDTDPGARILGEFAIGTNYDIQRFSKNILFDEKIGGTVHM
                     AIGAGYPETGNNNTSAVHWDMICDLRQGGEVRADGELLLKDGQFVV"
     gene            789..2090
                     /gene="aroA"
                     /locus_tag="VHR64_07700"
     CDS             789..2090
                     /gene="aroA"
                     /locus_tag="VHR64_07700"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX3302750.1"
                     /translation="MSDSLRIVPLARPIDAEVRVPGSKSITNRALLIAGLAAGESTLI
                     GMLFSDDTRYMAEALSGLGLAVETDQATETARVVGGNGDFPNGRADLYVGNSGTTMRF
                     LTAAIAVGRGEYRVDGNERMRSRPIGPLLHALHDLGSSAWSEKNNDCPPVIVNANGLV
                     GGACSMEGGLSSQYFSAVLMAAPYSVQDVKIEVIGDLVSKPYMEITAAVMRDFGVEAE
                     LDRAQWRTFRVAAGQRYHGRTYQIEPDASNASYFFAAAAVTGGRVRVPGLGSRSTQGD
                     LGFVRALEQMGARVVMTDDATEVTGPADGKLRGIEIDMNAMSDTAQTLAAIAPYADGP
                     TTIRGIAHNRHKETDRIADLATELRRIGQEVVEFEDGLTVTPHPTRPAQITTYDDHRM
                     AMSFAVTGLRADGIEILDPGCTAKTFPDYFDRLAVATRSAR"
     gene            2126..3541
                     /locus_tag="VHR64_07705"
     CDS             2126..3541
                     /locus_tag="VHR64_07705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3302751.1"
                     /translation="MFEPATVVAAQENDSQSRGERLHRWLVVGGAIFVVLVSLWQSNA
                     DLRVSRYHPDESRWINRAPYLSELRHPMSSFWSDGYLIRGQPPMGSYITGLGLAIQGY
                     DLTSNGPWNFSFGNDGDINWNVTYGNMPPADYILAARHTSMVIGALTCLCVYLIVTLL
                     SNWIGGVIAGLFMGVHPLSVYLFTLGVSDAAFTLLVALSVLAAIWLARRPSWGRAILF
                     GVVLGIGTSLKLSPIFVAFGLAAIGVGILVGPHLARFRPVGWVWRRSGADDWSIHRLG
                     WMLIALPVIAGAFFVASYPYLWADPVGRTNDLFEFRRAEMENQSRIWGDAAIKSRAEA
                     LTRTWDMLEVRYSATGKFLVKAGILEPRTGNPNGEEPGYDLPFALTGLAIFAATALWR
                     GFRSPQFLAFLTLMGQAGIILLGLNVDFDRYYLPLVLTFAIGLGVGIGEVTGWLLRVR
                     ERTPREARVPHSVEADPIRAS"
     gene            complement(3538..4188)
                     /locus_tag="VHR64_07710"
     CDS             complement(3538..4188)
                     /locus_tag="VHR64_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302752.1"
                     /translation="MTNERMMISRRMFVQHSALIATGVAIGVVARGQFALGTSVVAAT
                     PDATATREAELEELHALQTQVAQPLVCTPAPTSTPSPTATPVPPSQTGMPVAYGAIWT
                     ITVLGIFPAMTPPDSKVTGKLMQVNLAASHNAPVGQLPPLTELRLVDSSGRFSVANTA
                     LNQAAFGVGWGLKISPGVTENLSVVFDVAADAGDSFILESNADPTFRVAMTVEQRG"
     gene            complement(4359..4541)
                     /locus_tag="VHR64_07715"
     CDS             complement(4359..4541)
                     /locus_tag="VHR64_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX3302753.1"
                     /translation="MVPYVIAWVKDQIEREEEGQGLVEYALILVLVSIAAIVALTTLG
                     SKLSPIFDKVSSALTF"
BASE COUNT          814 a         1455 c         1483 g          850 t
ORIGIN      
        1 ctcggtcgac ccggcccgcc agcagttctt ccagggcgcc cgtcgcgagc tcctccagac
       61 ctatttgcgc cgcgatgccg aaggcttgtt gaactgggtc ctcacccttt atccgaccga
      121 cgcctatgcg caggatgcgg atatgtcgac ggatgagttc accgagttcg tcttccgggc
      181 cggcaagctc cacaccgacg acccgatttc ggcctggaag gcggagactg atttcgccca
      241 gcggattgtc aactggctag atggcaagca ggagatccac ctgaccggcc cgggcaccga
      301 cctgcacgtc agcgtcgcga accggatctg gatcaactcg ggcgatgcga ccaagaactt
      361 cccggacggc gaggtcttta ccggtccggt cgaggacggc gtcaacggcg tggtgagctt
      421 cagcttcccg gtcgtgaccg ccggtcgcga gatcgaggga atcgtccttc gcttcgagca
      481 tggcaaggtg gcgcaggcga ccgcgacccg gggtcaggag tacctcgagg cggtgctcga
      541 tacggatccc ggcgcgcgca tcctcggcga gttcgcgatc ggcacgaact acgacatcca
      601 gcgcttctcg aagaatatcc tcttcgacga gaagatcggc ggcacggtcc acatggcgat
      661 cggcgccggc tatccggaga ccggcaacaa caacacatcc gccgtgcact gggacatgat
      721 ctgcgaccta cggcaaggcg gcgaggtccg ggcggacggc gagctgcttt tgaaggacgg
      781 ccagttcgtt gtctgattcg ctgcgcatcg ttcctctcgc gcgtcccatc gacgccgaag
      841 ttcgcgtgcc cggttcgaag agcatcacga accgggcgct gctgatcgcc ggtctcgcgg
      901 ccggcgagag cacgctgatc ggcatgctct tcagcgacga cacgcggtac atggccgagg
      961 cactcagcgg cctcggcttg gccgtcgaga ccgatcaggc gaccgaaaca gcgcgggtgg
     1021 tcggcgggaa cggcgacttc ccgaacggca gggccgatct ctatgtcgga aattccggca
     1081 cgacaatgcg ctttctgact gccgcaatcg cggtcggccg gggcgagtac cgggttgatg
     1141 gcaacgagcg gatgcgctcc cggccgattg gcccgctgct gcatgcgctc cacgatctcg
     1201 gctcatccgc atggagcgag aagaacaacg actgcccgcc ggtcatcgtc aacgccaatg
     1261 ggctggtcgg cggcgcgtgc agcatggagg gcgggctgag cagccagtac ttcagcgccg
     1321 tcctgatggc cgcgccttac tccgtgcagg acgtcaagat tgaagtgatc ggcgatctcg
     1381 tctcgaagcc gtacatggag atcaccgccg ccgtgatgcg cgatttcggc gtcgaggcgg
     1441 agctggaccg cgcgcagtgg cgaacatttc gcgtcgcggc gggtcagcgc tatcacggtc
     1501 ggacgtacca gatcgaaccg gatgcctcca acgcttcgta tttctttgca gcggcggcag
     1561 tgaccggcgg acgggtccgg gtgccgggtc tcggcagccg ctcgacgcag ggtgacctcg
     1621 gtttcgtccg cgcgctggag caaatgggtg cgcgcgtcgt catgacggac gacgcgaccg
     1681 aggtgaccgg tccggccgac ggcaaattgc gggggatcga gatcgacatg aatgcgatgt
     1741 ccgacacggc ccagacgctg gccgcgattg cgccgtatgc cgacggcccg accacgatcc
     1801 gggggatcgc gcacaaccgg cacaaggaga ccgaccggat cgccgacctc gccaccgagc
     1861 tccgccggat tgggcaagag gtcgtcgagt tcgaggacgg gctgacggtc acgccgcatc
     1921 cgacccgacc ggcgcagatc acaacctacg acgatcaccg catggcgatg agcttcgcgg
     1981 tgactggatt acgggccgac ggaatcgaga tcctcgatcc cggttgcacg gcaaagacct
     2041 tcccggacta cttcgatcgg ctggcggtgg cgacgcgctc ggcacgctga ggacgccgac
     2101 tccgccggct gatagactcg ccaccatgtt tgagcccgcc acagtagtcg ccgctcaaga
     2161 gaacgactcg caatcccgcg gcgaacgcct gcaccgctgg ctcgtggtgg gcggcgccat
     2221 tttcgtcgtg ctggtcagtc tctggcagag caacgccgat ctgcgcgtca gccgctatca
     2281 cccggacgaa tcgcgctgga tcaaccgcgc gccgtatctc agcgagctgc gccatccgat
     2341 gagctcgttc tggtcggatg gctacctgat caggggccag ccaccgatgg gctcgtatat
     2401 caccgggctg ggtctggcga tccagggcta cgacctcacg tcgaatgggc catggaactt
     2461 cagctttggg aatgacggcg acatcaactg gaacgtcacc tacggcaaca tgccgccggc
     2521 cgactacatc ctcgctgcgc gccacacctc gatggtgatc ggcgcgctga cgtgcctgtg
     2581 cgtctacctg atcgtgacgc tgctctcgaa ctggatcggc ggcgtgatcg ctggcttgtt
     2641 catgggcgtg cacccgttgt ccgtgtactt gttcacgctc ggggtctcgg acgcggcctt
     2701 cacgttgctc gtcgccttgt cggtgctggc cgcgatctgg ctggcgcggc gaccgtcgtg
     2761 gggccgggca atcctgttcg gcgtagtcct tgggatcggg acctcgttga agctgagccc
     2821 gatcttcgtt gccttcggtc tggcggcaat cggcgtcggc atcctcgtcg ggccgcatct
     2881 ggcccggttc cggccggtcg gttgggtttg gcgaaggtcg ggtgcggacg actggtcgat
     2941 ccaccgcctg ggctggatgc tgatcgcgct accggtgatt gcgggcgcct tcttcgtggc
     3001 gtcatacccg tatctctggg cggatccggt gggtcgtacc aatgaccttt tcgaattccg
     3061 gcgcgcggag atggagaacc agagccgaat ttggggcgat gccgcgatca agagccgggc
     3121 cgaggcgctg acccggacct gggacatgct cgaggtgcgc tacagcgcga ccggcaagtt
     3181 cctggtcaag gccgggattc tcgaaccgcg aactggcaat ccgaacgggg aagagccggg
     3241 ttacgatctg ccgttcgcgc tgactgggct ggcgatcttt gcggcgaccg cgctctggcg
     3301 cggcttccgg agcccgcaat tcctcgcatt cctgacgctg atgggccagg cgggcatcat
     3361 cctgctcggg ttgaacgtcg acttcgaccg ctactacctg ccgctggtgc tgaccttcgc
     3421 gatcggcctg ggcgtcggca tcggcgaggt caccggctgg ctcctgcgcg tgcgtgagcg
     3481 cacgccccgc gaagcgcgcg tcccccactc agtcgaagcc gaccccatcc gcgccagcta
     3541 accccgctgc tccaccgtca tcgccacccg gaacgtcgga tcggcattgc tttccaggat
     3601 gaatgagtcg ccggcatcgg cggcaacgtc gaacacaact gaaagatttt cggtaacccc
     3661 cggagaaatt ttcagacccc acccaacacc gaaggcagcc tgatttaggg ccgtattcgc
     3721 aacagaaaac cgtccactgc tatccactaa gcggagttca gtcagcggtg gaagctggcc
     3781 gacaggggca ttgtgggacg ccgctagatt tacttgcatg agcttccctg tgaccttgga
     3841 gtccggaggg gtcattgctg ggaaaatacc caggaccgtg atcgtccaga tcgcgccata
     3901 agccacgggc ataccagttt gcgatggtgg cactggagtt gccgtcggtg aaggcgtgga
     3961 agtcggtgcg ggggtgcata cgagaggttg ggcaacctgc gtctggagcg cgtgcaattc
     4021 ttcgagctcc gcctctcggg tcgcagtcgc gtccggtgtg gccgcaacta ccgacgtgcc
     4081 gagggcgaac tgtcctcgcg ccacaacccc gatagcgaca ccagttgcga tcagggcgga
     4141 atgctgcaca aacattcgac ggctgatcat cattctctcg ttggtcattg ggtttggtcc
     4201 tgcttgtatt gctcgtacga cacgatctgg attctgctca cgattcctgc cgccgaaaat
     4261 cacccgaaag tgcctttgcc gcgaaatgca aagaccccgg ccaaacggcc ggggtctttg
     4321 gtttgagact ggaccagggt ccggccccgt tttcctgatt agaaggtcag ggccgaggag
     4381 accttgtcga agatcggcga aagcttgctg ccgagggtcg tcagcgcgac gatcgcggcg
     4441 atcgagacca gcaccaggat gagtgcatac tcgaccaaac cctggccctc ttcctcacgc
     4501 tcgatctgat ccttaaccca ggcaatcacg tacggaacca ttgcagtttt cctccctgca
     4561 taccaacgaa cgaaaagtgc tgataaccat gtcgcccgtt tt
//
LOCUS       DATAOZ010000293         9691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6958630, whole genome shotgun sequence.
ACCESSION   DATAOZ010000293 DATAOZ010000000
VERSION     DATAOZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6958630"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..780)
                     /gene="hpt"
                     /locus_tag="VHR64_07720"
     CDS             complement(229..780)
                     /gene="hpt"
                     /locus_tag="VHR64_07720"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX3302754.1"
                     /translation="MGRKEAGVERVLVEPAELAARVRDLASQVDSWYPAESPILIGVL
                     NGAVVFMTELMQAMTVPVSIDFMAVSSYGSSTQSSGVVQILKDLSVSIEGRDVLVVED
                     IVDSGLTLQYLLDYLQRRNPKSLRVVALLRKVKSSALAVPVDLVGFEIPDEFVIGYGL
                     DYAGLYRNLPYIGVLSPAAIMGN"
     gene            complement(804..1787)
                     /gene="tilS"
                     /locus_tag="VHR64_07725"
     CDS             complement(804..1787)
                     /gene="tilS"
                     /locus_tag="VHR64_07725"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX3302755.1"
                     /translation="MQRGAVSGVVQRFLAFLDDQELTGRSVGVAFSGGRDSSALAISA
                     IEARNAGRLSPVLVHVDHGVRPDSKEDRAVVREAADRLGAELMSVDLDSLGTHSTEAE
                     MREARYLSLHQALAPLGVDTVLTGHHARDQAETVLLHLARGQGLEGATGISPREPLRF
                     GDIAIHLVRPFLHEDPVALADLVATYGLPFVEDPTNLLVDRARNLVRHQVLPVLCDIN
                     SGAVINIARSTDILRDEDEFLAAIADSATTAVTHASVLDGVALNHETVAIQRRVIRRW
                     VTRETAMELTFDRTEAIRELAQAGTGNVMLEIGEGWIARQIQRQITLHRSK"
     gene            complement(1818..1889)
                     /locus_tag="VHR64_07730"
     tRNA            complement(1818..1889)
                     /locus_tag="VHR64_07730"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1855..1857),aa:Met,seq:cat)
     gene            2033..4294
                     /locus_tag="VHR64_07735"
     CDS             2033..4294
                     /locus_tag="VHR64_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX3302756.1"
                     /translation="MTSPLLEGLNAEQQAAVLATDGPVLVVAGPGSGKTRVLTHRIAY
                     VIQELGASPDEILAVTFTNKAAREMRERIDHLLGSSGATTGLWMGTFHSMGVRILRAH
                     PGVVADRLGILPNFLIYDDADQIGVAKQAITAIGQDPKKVAPRRMLSRISAAKSQLFT
                     PHEFRESEIQTYDDEIVARVFEEYERLLRRNNALDFDDLLSQPIRLFDAAANVLDHYQ
                     SKFRYILVDEYQDTNRVQYVMVSALAGKHRNLFVVGDPDQSIYAWRQADIRNILDFER
                     DYPDARRIDLEINYRSTARIVAAADNVIKANTQRLDRKLRTINDEGAPISVRGLTDQN
                     HEARFIVDEIRRLVVSGYRVGDIAVMYRTTAQSRVLEEAFRQSDVRYRIVGGVRFYER
                     AEVKNALAAMRLLYNPADQVSLQRVLDTMPIGRGLGPKALEAIEEWARPLGLPLLDGF
                     MALNTPDSEYGSPPLSGATRSAAARFGEAFTNLRKQMMELPLLDLFDAVMEETGYASS
                     FRQDIEEDMDRQANVLELRSDIARYNELAPGAGLAAYLEQVALIADVDSMDRDRSEAC
                     TLITLHSAKGLEYPVVFIAGVEEGLLPISRAIEVEYDDPSQVEEERRLFYVGITRAET
                     LLYLTYTGMRMSYGRLDSSVPSRFLDALPQEHVKVLAARHTLAASGTGRLLDRARTTT
                     RSFSSPVAAPVRVNPIVPTYTVGQRVFHAQFGEGTVSEVVARNSDQEVAVEFARHGRK
                     RLLASIAPLDLVD"
     gene            4338..6215
                     /gene="dnaG"
                     /locus_tag="VHR64_07740"
     CDS             4338..6215
                     /gene="dnaG"
                     /locus_tag="VHR64_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX3302757.1"
                     /translation="MASDAVADVRDRTDIVNLVSDYVQLKKAGRNFKGLCPFHTEKTP
                     SFIVFPDSGNFKCFGCGKGGDVFTFYKEVERVDFREALEELARRAGIELKSAPKPSPE
                     RDAHRQKLTELNELAATFYATQLRSAAAGERGRRTVEQRGLSAEVVEAFQIGVAPEGW
                     DTLAQFFAARGVDPDRAVDAGLLTRRETGGYYDRFRNRLMFPIRDRDGNVVGFGGRAF
                     GDEQPKYLNTPQTELFDKSHLLYGLDLARDEIRRQDKAVVVEGYMDVIAAHQFGYRNV
                     VASMGTAITESQLGLLKRFSKNVVLALDADAAGQAAMTRALDALPDGESDAHPVPSPR
                     GLIHYEKRLSVNINVLALPSGKDPDEMIRADPDQWPSVVNEARPFLAFYIDRIAEGVD
                     LTDIREKTAAVHRAAVLLSLVPDGVEQRHYVEVLANRLRMSERKSIEEAIRQARKQNS
                     ISAPPLPSPPVPRKSSVEDHLAALILAYPAILADFAAELNPNELTDARNRELVIQVAA
                     SHGEPTADPADHLQTHRDYLASLLSNRITMNRMDVMREAQRTLEKLRQDQITNSITEL
                     QFDIAVAEREKDEPTIRLLLTVLTGLAAEKRTTAPRTSPVFVDTRTPRVVRKSRFKVV
                     E"
     gene            complement(6247..6324)
                     /locus_tag="VHR64_07745"
     tRNA            complement(6247..6324)
                     /locus_tag="VHR64_07745"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6287..6289),aa:Pro,seq:cgg)
     gene            complement(6493..6654)
                     /locus_tag="VHR64_07750"
     CDS             complement(6493..6654)
                     /locus_tag="VHR64_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302758.1"
                     /translation="MRSLLDASLQVAHGASADTGTLGQCLLGQGSGDPMLPEEVCKLN
                     VARLPVKLQ"
     gene            6670..8979
                     /locus_tag="VHR64_07755"
     CDS             6670..8979
                     /locus_tag="VHR64_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3302759.1"
                     /translation="MLADALDLTGSDRTALAVASRPAHVSPTESELQAPVRAAVPVGL
                     PVPSTPLVGRADIAKLAASLIRDDRARLLTLTGPGGVGKTRLAVAIATELGDAFPDGI
                     AWIELAPVSDPLLIPGTILRSLGEVQSRDSSLREVLSQRQMLLVLDNCEHVREGAAQL
                     VAELLPYCHDLFVLATSRSSLRLTAERLLPVEPLSLPEPEPSQTVESVSQSDAVRLFV
                     LRARAMLPGFSLTPDNAEDVAAICRRLDGLPLAIELAAARVRVLSPAALLGQLDTRFR
                     ILTGGPADLPARHQSLQATLDWSYDLLNASLQASFRALAVFAGGFTLDAACAVIGEED
                     PLATLEDLEQLVDQNLVKLMPAAGDDLRFTMLETVGEYGREQCFVSGDEPEVRDRHVA
                     WFMQLAERAEPELAGPHQGAWLRLLDADRDNLREALSWLHVNERRDDLLRLGTALWPY
                     WARRGSDDEVRGWLDRALDPNNGDPATLAKSYHRVGNLAVDLGDYPRARDMFTSALGI
                     AKSIPDQQGIANALNGLGVVASDTGDLDEARSLHGRSLAIRRGTGDQVGVARSLYNLG
                     LIDAANGNNASARLHLSEALEIKESLGDAAGIAFSQWALAAIDIRDGNLARAHDLLYS
                     SLNVFRELGDGYGQAFVLAELGALAHAQNDDRSSLTHLLEALEIQEDLRDDWGILATL
                     EGLAMAASGLNQAATAAKLLGATEVIRRSIRVPRAPVKEVQISQLRLDCLDVLGLEDF
                     EHEFARGGVMSLDQAITAARTGFSLDLGT"
     gene            9059..9442
                     /locus_tag="VHR64_07760"
     CDS             9059..9442
                     /locus_tag="VHR64_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302760.1"
                     /translation="MTLLQQKGKTMPVGPKDSGEEILELASGNSVHYGRQVGFTWTVT
                     FTVNAGTVLIDGHGKRDETASPLSCANRCKIKLTPASFDSGTDPVEVDQLLTQGDTFT
                     YEGGLVLAVTALNGPATIHRSWTTA"
BASE COUNT         1909 a         2968 c         2971 g         1843 t
ORIGIN      
        1 ctgctttcgc gtcgttgacg acctgggaga tcgtcgtcgg cgtcgtattt tcgccgctac
       61 gggcaaacgc aaaccagacc agtgaaagcg ccaccagcag gccaagccag atgactccgg
      121 ggcgatagcg gcgaaggttc atcatgcggg gtagtcctca gcatgcagat tgacctgcac
      181 gttcaggata gcgttcaaac aagctctttc gcgcagtata gcaaccgctc agttgcccat
      241 tattgcggcg ggtgacaaaa cgccgatata gggcagattc cgatacaggc cagcataatc
      301 cagcccgtat ccgatcacga attcgtcggg aatctcgaac ccgaccagat cgaccgggac
      361 ggccagagcg ctgctcttga ccttcctcaa caacgcaacg actcgcaagc tcttcgggtt
      421 tcggcgctgc agataatcga gcaagtattg gagcgtcagg ccggagtcga cgatgtcctc
      481 gacgaccagc acatccctgc cctcgatcga cacgctcagg tccttcaaaa tctggacgac
      541 gccggacgat tgcgtgcttg acccgtagct ggagacggcc atgaagtcga tgctgaccgg
      601 cacggtcatt gcctgcatca actcggtcat gaagacaacc gcgccattga gtacaccgat
      661 cagaatcggc gactcggcgg gataccaact gtcgacctgg ctcgccagat ccctgacccg
      721 cgccgccagc tccgcgggct cgaccagaac ccgctcgacg cccgcttcct ttcgccccaa
      781 gattgtgctt cccttcgata tcatcatttg ctccggtgaa gcgtaatctg tcgctggatc
      841 tgacgcgcaa tccagccctc gccgatctcg agcatcacgt ttccagttcc agcctgggcc
      901 agttcccgaa tggcctcggt ccggtcgaat gtaagctcca ttgcggtctc gcgagtcacc
      961 caccggcgaa tgacacgacg ttgaattgcg acagtctcgt ggttcagcgc cacgccatcg
     1021 aggaccgagg catgtgtgac ggcggttgtg gccgaatcgg caatcgcggc aagaaactcg
     1081 tcttcgtcgc gcaggatgtc tgtcgagcgt gcgatgttaa tgacggcgcc ggaattgatg
     1141 tcacacagca cgggcaggac ctggtggcgg accaggttgc gcgctcgatc gacgagcagg
     1201 ttggtcgggt cctccacgaa cggaagacca taagttgcga cgagatcagc gagtgctacc
     1261 ggatcttcgt gcaggaacgg ccgcacgagg tggatcgcga tatcaccgaa cctgagcggt
     1321 tcgcgcgggc tgatgccggt cgcgccctcg aggccttgcc ctctggcgag atgcaggagc
     1381 acggtctcgg cctgatcgcg cgcgtggtga ccggtcagga ccgtatcgac gccgagcggc
     1441 gcgagtgcct gatggagaga cagatatcgc gcctcccgca tctctgcctc agttgaatgg
     1501 gtaccaagcg agtccagatc gaccgacatc aattcggcgc ccaaccgatc ggcggcctcc
     1561 cggacgactg cgcgatcttc tttgctgtcc ggccgaacac cgtggtcgac gtggacaaga
     1621 accggcgaga ggcggccggc attgcgagcc tcgatggcgg agatggcgag cgcgcttgaa
     1681 tcccggccgc ccgagaaggc gacaccgacg ctccggccgg tcaattcctg gtcatcaagg
     1741 aaggccagaa accgctggac gacccctgaa acggctccgc gctgcatatc aaatccaggg
     1801 agttgatgag gggaaagtgc cgacgggtgg attcgaacca ccggccaaag gcttatgagt
     1861 cctttgctct gccactgagc tacgtcggcc aaaacgcacc gggtaggcta ccaattgtga
     1921 cccccctgcg tcaacgttcg gagcaggtct gccgggtgcg aagccgggcc agcgaccgat
     1981 ggtagaatcg tcagtcgaaa cacgtgttcg caccgcaaag agcatccact gcatgacatc
     2041 tcccctccta gaaggtctca acgccgagca gcaagctgcc gtccttgcga ccgacggtcc
     2101 agtgctggtc gtggccggac cgggctccgg caagacccgg gttctgacgc accggatcgc
     2161 ctacgtgatc caggagctcg gcgcgtcgcc ggacgagatc ctggcggtga ccttcaccaa
     2221 caaggccgcg cgggagatgc gggagcgaat tgatcacctg cttggctcat cgggcgcgac
     2281 caccggcctc tggatgggca catttcactc gatgggtgta cggatcctcc gcgcgcaccc
     2341 gggcgtggtc gccgatcgcc tcggaatcct gccgaatttc ctgatctatg acgatgccga
     2401 tcaaatcggc gtggccaagc aggcgattac cgcgatcggc caggacccga agaaggtcgc
     2461 gccgcgccgg atgctctcgc gcatctcagc cgccaagagc cagctcttca cgccgcacga
     2521 gttccgcgaa agtgaaatcc agacttatga cgacgagatc gtggcgcgcg tcttcgagga
     2581 gtacgagcga ctcctgcgcc ggaataatgc gctcgacttc gacgacctgc tgagccagcc
     2641 gatccgcctg ttcgatgcag cggctaatgt gctcgatcac tatcagtcga aattccgcta
     2701 catcctcgtg gacgagtacc aggacacgaa tcgagttcag tacgtgatgg tttcggcgct
     2761 ggcagggaag catcgcaacc tgttcgtcgt cggtgatcct gaccagtcga tctacgcctg
     2821 gcggcaggcc gacattcgca acatcctcga cttcgagcgt gattaccctg atgcgcgccg
     2881 catcgacctg gagatcaact accgatcgac ggcgcgaatc gtggccgcgg ccgacaacgt
     2941 gatcaaggcg aatacgcagc ggctcgatcg caagctccgc acgatcaatg acgagggcgc
     3001 gccgatctcc gtccgcgggt tgactgatca gaatcacgag gcgcgtttca tcgtcgatga
     3061 aatccggcgc ttggtcgtct ccggctatcg cgtcggcgac attgccgtca tgtaccggac
     3121 aacggcccaa agccgcgtgc tggaggaggc attccgccaa agcgacgtcc gataccgaat
     3181 cgtcggcggg gtccgattct acgagcgtgc ggaagtcaag aacgcgctcg cggccatgcg
     3241 gcttctctac aacccggccg accaggtcag tctccagcgc gtcctcgaca cgatgccgat
     3301 cggacgcggg ctcggaccaa aggcgctcga ggcgatcgaa gaatgggcac gtccgcttgg
     3361 gttgccactg cttgacggct ttatggcgct caacaccccg gattctgaat acggttcgcc
     3421 gccactttcc ggagccaccc gatcggccgc ggcccgattc ggcgaggcat tcactaacct
     3481 ccgcaagcag atgatggagt taccgctcct ggacctcttt gacgcggtga tggaggaaac
     3541 cgggtatgcc tcctcgttcc gtcaggacat cgaggaggac atggatcgcc aggcgaatgt
     3601 gctcgagctc cggtcggata tcgcgcggta caacgagctc gcgcccggcg ccggcctggc
     3661 cgcctacctc gagcaggtcg cgctcatcgc cgatgtcgat tcgatggacc gggaccgatc
     3721 cgaagcatgc acgctgatta cgctgcattc ggcgaagggc ctggaatatc cggtcgtgtt
     3781 catcgccggt gtcgaggagg gcctgctccc aatctcgcgg gcgattgagg tcgagtacga
     3841 cgacccgagc caggtcgagg aggaacgacg cctcttctat gtcggcatca cccgagccga
     3901 gacgcttctc tatctcacgt acacgggcat gcgcatgagt tacggtcggc tcgactcaag
     3961 tgtgccgtct cgtttcctgg atgccctgcc gcaggagcac gtcaaggtgc tggcggctcg
     4021 ccacacgctg gccgcgtcgg gaaccgggcg cctacttgat cgggcgcgaa cgacgacgcg
     4081 ctcgttctca tcgcctgtcg ccgcaccggt gcgggtcaac ccgatcgtgc ccacctatac
     4141 agttggccag cgcgtctttc acgctcaatt cggcgagggc acggtatccg aggtcgtcgc
     4201 gcggaacagc gaccaggaag ttgccgtcga gtttgcccgt catggacgaa agcgcctcct
     4261 cgcgagtatt gcgccactcg acctggtgga ctagaacaaa tacagatccc gcagcgcccc
     4321 gctgaaggac acgatcaatg gcatccgacg cggtagcaga cgtcagggac cggactgaca
     4381 tcgtcaatct ggtatctgat tatgttcagc tcaagaaagc ggggcggaac ttcaaggggc
     4441 tttgcccctt ccacacggag aagacgccct ctttcatcgt cttccccgat tccggcaact
     4501 tcaaatgctt cggatgcggc aaggggggcg atgtctttac cttctacaag gaggtcgagc
     4561 gcgtcgattt ccgcgaagcg ctggaggaac tggctcgccg ggccggcatc gagctcaaat
     4621 ccgcaccgaa gccatcgccc gagcgggacg cccatcgcca gaagctgacc gagctgaatg
     4681 agctggccgc taccttctac gccacgcaat tgcgctcagc ggcagccggg gagcgcggtc
     4741 gccggacggt cgagcagcgc ggactgtcgg ccgaggttgt tgaggcgttc cagatcgggg
     4801 ttgcgccgga gggttgggac acgctcgccc agttctttgc cgcgagaggc gtcgatcccg
     4861 accgcgccgt cgatgccggt ctgctgactc gacgggaaac tggcggctac tacgatcggt
     4921 tccggaaccg gctcatgttt ccgatccgcg atcgtgatgg aaatgtagtc ggattcggag
     4981 ggcgcgcgtt cggcgatgag cagccgaagt acctgaacac gccgcagacc gagctcttcg
     5041 acaagagcca cctgctctac gggcttgacc tcgcccgcga cgagatccgc cgccaggaca
     5101 aggcggttgt cgtcgagggg tacatggacg tcattgccgc gcaccagttc ggctaccgga
     5161 atgtggtggc gtcgatgggc acggccatca ccgagtcgca gctcggcttg ctcaagcggt
     5221 tcagcaagaa cgttgtcctg gccctcgacg ccgatgcagc cggccaggca gccatgacgc
     5281 gcgccctcga cgcgctgccg gacggagaat ctgacgccca cccggtgccg tcaccacgcg
     5341 gcctgatcca ttacgagaag cgactcagcg tcaatatcaa tgttcttgcc ctcccgagcg
     5401 gcaaggaccc ggacgagatg atccgcgctg atcctgacca gtggccgtcg gtagtgaacg
     5461 aggcaagacc attcctcgcc ttctacatcg accgcattgc cgagggtgtc gacctcacgg
     5521 acatccggga aaagaccgcg gcggtgcatc gcgcggccgt gctcctctcc ctggttccgg
     5581 atggagtcga gcagcgccat tatgtcgagg tgctggcgaa tcgattgcgc atgtccgagc
     5641 gcaagtcgat cgaggaagcc attcgccagg ctcggaagca gaattcgatc tccgcgccac
     5701 cgctgccgtc gccacctgtt ccacgaaaga gcagcgtgga agaccacctg gcggccttga
     5761 tcctcgccta cccggcgatc cttgccgact tcgccgccga gctcaacccg aacgagctga
     5821 ctgacgcacg taatcgcgaa ttggtgatcc aggtggccgc gagccatggc gagccgactg
     5881 cggaccccgc tgatcacctg caaacgcacc gggattacct ggcgtcgctt ctctcaaatc
     5941 ggatcaccat gaaccggatg gacgtgatgc gagaggcaca gcgcacactc gaaaagttgc
     6001 gtcaggacca gatcaccaat tcgatcacgg agctgcagtt cgatatcgct gtcgctgaac
     6061 gcgaaaagga tgagccgact attcggctcc tgttaactgt tttgactggc ctggctgcgg
     6121 aaaaacgaac gaccgcaccg cggacaagcc ccgtgttcgt ggataccaga acgccgaggg
     6181 tggtacgcaa aagcaggttc aaggtcgtgg agtagctgca gaagggggcg agtgtctcgg
     6241 gtgaggtggt cggggagaga ggatttgaac ctccgacctc agcgacccga acgctgcgcg
     6301 ctagccggac tgcgccactc cccgccgacg gcaaattcta catgaccaga cttgctccgg
     6361 caatataagt gctgcccttc gcgaccctcg ttctactcga accaattacc tttgacgttg
     6421 tgatgaaact agcaacgtcg taggatgcgg gcattcaccg gcagtctatg agctctggaa
     6481 ccgccagcca tgtcactgga gctttacggg caaccgcgca acgttcaatt tgcagacctc
     6541 ctccggcagc atcggatcgc cgctgccttg tcccaggagg cattggccga gcgtgccggt
     6601 atcagcgctc gcgccgtgag cgacctggag cgaggcgtca agcagcgacc gcatcttgag
     6661 acagttcgaa tgcttgccga tgcgctcgac ctcacgggat ccgatcgaac ggcactcgcg
     6721 gtggcatcac gccccgcgca cgtctcgcct acggaatcgg aacttcaagc tcctgtcagg
     6781 gctgcggtac ctgttgggct tccagttccg tcaacgccgc tcgtcggacg agccgatatt
     6841 gccaagttgg cggccagcct gatccgggac gatcgggctc gcttgctgac actcactggt
     6901 cccggcggcg tcggcaagac acgcctcgcc gtggcaatcg cgactgaatt aggtgacgcg
     6961 tttccggacg gaatcgcctg gatcgagttg gccccggtct ccgatccgct gctgataccg
     7021 ggcactattc tcagatcgct cggcgaagtt caatcgcgcg atagctcgct ccgtgaagtc
     7081 ctctcgcagc gtcagatgct gctcgtgctc gataactgcg agcatgtccg ggaaggcgcc
     7141 gcgcaacttg tcgcggagct cctcccctac tgccatgacc tgttcgttct tgcgacgagc
     7201 cgatcgagcc tgcgcctgac cgcggaacgc ttgcttccgg ttgagcctct ctcgttgccg
     7261 gagcctgaac ccagccagac ggtcgagtct gtcagtcaaa gcgatgcggt ccggttattt
     7321 gtattgcgtg ccagggccat gctgccaggt ttttcattga ctccggataa cgccgaggac
     7381 gttgccgcca tttgtcggcg acttgacggg cttccgctgg caatcgagct tgcggcagcc
     7441 cgggttcgcg ttctttcacc ggcggcgctt ttggggcaac ttgacacgcg attccgcatc
     7501 ttgacaggcg gaccggccga tctcccggca cgccatcagt cactgcaagc gacactcgac
     7561 tggagttacg acctcctgaa tgcgtcactt caagcgagct ttcgtgcatt ggcggtcttc
     7621 gccggcggat tcacccttga tgcggcatgc gctgtgatag gcgaggagga tccgctggcg
     7681 acacttgagg atctcgagca gctcgtcgac caaaatctgg tcaagctgat gccagctgcg
     7741 ggcgacgacc ttcgtttcac gatgctggag acggtgggcg agtacggacg cgagcagtgt
     7801 tttgtgagtg gagacgagcc ggaggtgcgc gatcggcacg tggcctggtt catgcagctt
     7861 gcggagcgcg ccgagcctga gctcgcgggg ccacatcagg gcgcgtggct tcgcctgctg
     7921 gatgcggacc gtgacaatct ccgtgaggca ttgtcctggc tgcatgtcaa cgagcggcgg
     7981 gatgatctgc tccggctcgg caccgcgctc tggccgtact gggcccgtcg gggcagcgac
     8041 gatgaggtcc gaggatggct agatcgggca ctcgatccaa acaacgggga tcctgccaca
     8101 ctggcgaagt cttaccaccg ggtgggcaac cttgcggtgg accttggcga ttacccgcgc
     8161 gcccgcgaca tgtttacctc cgcgcttgga atcgcgaagt cgattccgga tcagcaaggc
     8221 atagcaaatg cgctcaacgg gctgggggtc gtcgcgtcgg atactggcga tctggacgaa
     8281 gcgagatcac ttcatggccg ttcactcgca atacgaaggg gaacggggga tcaagtaggc
     8341 gttgcgcgat ccctctacaa cctcgggctc atcgatgctg ccaacggaaa caatgcgagt
     8401 gctcggcttc acctttcgga ggctcttgaa atcaaggaat ctttgggcga tgccgccggt
     8461 attgcatttt ctcaatgggc gctggcggca atagatattc gcgatggaaa tcttgcgaga
     8521 gcgcatgacc tcctctacag cagcttgaat gtctttcgag agcttggaga tgggtacgga
     8581 caggcattcg tgttagcaga gcttggtgcg cttgcccacg ctcaaaacga tgatcgatcg
     8641 tcactcacgc acctgctgga agcactcgag atacaggagg atctgcgcga tgactggggc
     8701 atattggcga ctctcgaagg cctcgcgatg gcagcgtcgg gactgaatca ggcggcgacg
     8761 gctgcaaagt tgctcggtgc aaccgaagtg atccgccgtt cgattagagt gccccgcgcg
     8821 ccggtcaaag aggttcagat ctcacaactc aggttggact gcctggacgt gcttggactt
     8881 gaagattttg agcacgagtt cgctcgcggc ggagtcatgt cgctggatca ggcgatcacc
     8941 gccgcacgaa ctggattttc gcttgatttg ggcacctagt ttgcgtcttc gcgccgctac
     9001 gtctttcgtt ttgacgcaaa tctgataaag tgcgaatagt tctcgacagg tcaggtcttt
     9061 gacgctgctc cagcagaaag ggaaaaccat gcccgtcgga ccaaaggaca gtggtgaaga
     9121 gattttggaa ctagcttcgg gaaattcggt gcactatgga cgccaggtgg gcttcacctg
     9181 gacggtcacg tttacggtga atgccggcac cgtgcttatc gatggccacg gtaagcgtga
     9241 cgaaactgca tcacctttgt cgtgtgcgaa cagatgcaag atcaagttga cgccggcgag
     9301 ctttgattct ggcaccgatc cggtagaggt cgaccaattg ctcacgcagg gtgatacctt
     9361 cacgtatgag ggtggactcg ttctggctgt cacagcgctc aacggaccgg cgactatcca
     9421 ccgaagctgg accaccgcct aagcgtgacg gtcgggccaa cctctaacaa tatgccgagc
     9481 tttcggaaga gggcatgcgt gctcaactgc caccggcaaa cttggggttg cgtgaatcgc
     9541 aaccgcacgg gctggcatga ataggcgcac ttcgcacaga gaacagcgcg ccactgtctc
     9601 ggaaagaacg ccgggggttc gaacccctcc gcggtcgttc tgccctattg ctgacattcg
     9661 cacgtcaggc ctttaaggat ttggccacac t
//
LOCUS       DATAOZ010000294         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7488443, whole genome shotgun sequence.
ACCESSION   DATAOZ010000294 DATAOZ010000000
VERSION     DATAOZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7488443"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            648..>1729
                     /locus_tag="VHR64_07765"
     CDS             648..>1729
                     /locus_tag="VHR64_07765"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX3302761.1"
                     /translation="MEKQTAVAPTAVAGEVVRIFLALELSRSSWLVASHTPAADKISR
                     HKLAAGDIDGLLALIERLRTRVEQKTGQPVQVISCYEAGYDGFWLDRKLKAEGVINHV
                     MDPASIQVDRRARRVKTDAVDADALLRALMAFCRGERKVCSMVRVPTRDEEDAKRLSR
                     EREQLIKERVRHVNRIKGLCATQGIYDYQPLKANRLERLDELRTGDGNPLPERLRNEI
                     GRQLERLKLLLELIGTVEAERDAVVKADKPASEADRKICALARLKGIGPEIATVLYNE
                     VFYRQFANRRDVAAYVGLTPSLFASGDLRHDQGISKAGNPRARATMVELAWLWVRHQS
                     GSDLTGWFLTRVSGIKGRIKRIAIVAL"
BASE COUNT          311 a          538 c          592 g          288 t
ORIGIN      
        1 ggcctacgcc ggcgtgttcg tctacggccg ccggcggcgc gaagcggttc ccggggatcc
       61 gccggagatg gtcgaccggc ggatcgcctt aacggagtgg gacatcgtcg tgcccgatgt
      121 ctaccctgga tacctgagct atgacgctta cctggccaac cgtcgggcgc tgaaagacaa
      181 cctctacaac ttcgcaaaca aggggcgcgg cgctccgcgc gaggggcttg ccctgctgac
      241 cggactggtc gtctgtggcc ggtgcggccg tcggatggcg gtgagctacg gctccgcgta
      301 tcacagctat cattgccgtc gtgcccaggt cgattatggg gaggcgctgt gccagtcctg
      361 cccggtcggc acgctggacg ccatcgtgac cgccgccttt ctggaggcga tggaaccggt
      421 cgggatcgag gccaccctgg ccgctctgga agcgctggaa cgtgagcagg aggctgtcgc
      481 ccggcattgg cagctgcgcc tggagcgtgc ccgctatgag gtggcgctgg cgcaacgcca
      541 gtatgatgcc gttgacccgg ggcctttaga tcctatggga cgtgctcagg ccgggacaat
      601 tagactcacg gcatcgatcg ctgaccaaac gctctggaga tcttgtcatg gaaaaacaga
      661 ccgctgttgc accgaccgcc gttgccggag aggtcgtccg gatttttctg gccctggaac
      721 tgagccgctc gagctggctt gtcgccagcc acacgcctgc cgccgacaag atcagccggc
      781 acaagcttgc cgccggggat atcgatggcc tcctggccct gatcgagcgt ctgcggacac
      841 gggtcgagca gaaaaccggg cagccggtcc aggtcatctc ctgctacgag gccggatacg
      901 acgggttctg gctggatcgg aaactgaaag cggaaggtgt catcaaccat gtgatggatc
      961 cggcaagcat ccaggttgat cgtcgagccc ggcgggtcaa gaccgacgcc gttgatgccg
     1021 acgcgctgct gcgcgccctg atggctttct gtcgcggcga gaggaaggtg tgcagcatgg
     1081 tgcgggtgcc gacccgggat gaggaagacg caaagcgcct cagtcgggag cgcgagcagc
     1141 tgatcaagga gcgcgtccgg catgtcaacc ggatcaaggg cctgtgcgcc acccagggga
     1201 tctacgatta tcaacccttg aaggcgaacc gcctggaacg gctggacgag ctgcggaccg
     1261 gcgacggcaa ccctctgccc gagcgcctca ggaacgagat cggccgacag ctcgagcgcc
     1321 tgaagcttct gctcgagttg atcgggaccg tcgaggcgga gcgcgacgcc gtcgtcaagg
     1381 ccgacaaacc ggccagcgag gcggaccgca agatctgcgc tctggccagg ctcaagggca
     1441 tcggaccaga gatcgcaacg gtgctgtaca acgaggtgtt ctaccggcag ttcgccaacc
     1501 ggcgcgacgt cgcggcctat gtcggattga cgccgagcct gttcgccagc ggcgatctgc
     1561 gccacgatca gggcatcagc aaagccggca acccgcgggc gcgggcgacc atggtcgaac
     1621 tggcctggct gtgggtgcgc catcagtccg gcagtgacct gaccggttgg ttcctgaccc
     1681 gcgtgagcgg catcaagggc cgcatcaagc gcatcgccat tgtcgctct
//
LOCUS       DATAOZ010000295         5431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8012311, whole genome shotgun sequence.
ACCESSION   DATAOZ010000295 DATAOZ010000000
VERSION     DATAOZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8012311"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VHR64_07770"
     CDS             <1..603
                     /locus_tag="VHR64_07770"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEX3302762.1"
                     /translation="RLGADVDIRHVDSNLDGYDVIVAPAIQIIDHRRAEHLASATRNA
                     SLVVGPRTGFRQPTGQVHPDGQPGPLRELLGCSLLNFDALRPKMTVTVDDEEVTLWAE
                     SYGSEQAEVMFRYDNGPLKGQAAVVRNGRAFTIGAFSPILIGRVFAHVLTEAGLEVTP
                     LDDGVRVARRGDRTVWLNFAEDDHRLPDGRTIDPVSFLID"
     gene            complement(614..1402)
                     /locus_tag="VHR64_07775"
     CDS             complement(614..1402)
                     /locus_tag="VHR64_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX3302763.1"
                     /translation="MEPSRTLERLGYKLYPQSINAYYRCPRKFHFRYIRNVRMPFEFK
                     PPLVIGGVTHKVLAAIFRDRRDHRPSSDIETYVTKYISFEKYPEGATDPMRDEHAPVV
                     TGHVERALQALPANAEVLHVEEEFAYSVRSRAIGAAVTVGAKVDVVIRHDDGLVDHID
                     FKTGKLSGDPIQNYLSRLTVGKQLSLTPDRLRTVNVLTGSGAYEVVPNSPAANEHIWN
                     IVQTQIHALSVDQEWHARPEPAICRFCDFRSKCDFADLDDEYGG"
     gene            1507..2436
                     /locus_tag="VHR64_07780"
     CDS             1507..2436
                     /locus_tag="VHR64_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX3302764.1"
                     /translation="MPNPPSRTDRARGVIVGLACGDALGAPVEFESRQSIAAKYPDGL
                     RDFTSGGWMNVVPGELTDDSRMMIDLAETLVRPGAVDLDHLGQRFVAWMAEGPKDIGN
                     TTRIAIEALRDGVHWSEAGELALTQRGPHGAASNGSVMRCAPVAIRFANNPDQLRAIS
                     IDSARITHAEPRCTWAAVALNQAIGHALNGGAIDELVDAATSGIEQPDVVRSIQNAPH
                     RSARDLKGSGYVLNAVEIAFWATITRPTFEEAVVSAVMIGDDTDTNAAVTGALAGAVH
                     GYGSIPERWRSTIYQHDRLVTLADQLLSASSSW"
     gene            complement(2406..3302)
                     /locus_tag="VHR64_07785"
     CDS             complement(2406..3302)
                     /locus_tag="VHR64_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX3302765.1"
                     /translation="MAQLDFSGQETAQRLRPGMSLPAAAAAFRADLERKRRPRNTIES
                     YIFDLTVLAHQIPTKAINQISADDIMRFLGEANTVSTRKRRLTSVRRFFRFLVDDAMV
                     LSIDPTEDFYPNRVELKIPEPLSSDEQEALLAAAATDEPWSPTAILLMMDAGLSRGEV
                     LKLERGNIDRYDPHAVVIHIVTDDPRKSNQNRTLRASERLAEAYGALLEARDPQGRLF
                     PVGFQAINGMVDRVRKRAGIGRSVTPRTLRDTFAAGRAVNGATEEELIHELGLADDVR
                     NRQSIRRFLALAAHQLELAESN"
     gene            3467..4219
                     /locus_tag="VHR64_07790"
     CDS             3467..4219
                     /locus_tag="VHR64_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3302766.1"
                     /translation="MPVQLTATYRDALEYAIELHAGQTRKGTDIPYISHVLAVSATVL
                     EFGGTEEEAIAALLHDAVEDAGGQATRAQIAERFGEAVAQIVDGCSDDSPEIGGQKKP
                     WRIRKNAHIAHLKKASSSVSLVTAADKLHNARALASDFRYLGDALWDRFNASREDILW
                     YYGAVVDVLDAGDMRLHDRMPDGTTTLTDLDRLIDELEVAVEDLIVDVGLMTMQSAFE
                     DALQENALDFFGPIDMQEQLEQSRGSNQPPAH"
     gene            4279..4665
                     /locus_tag="VHR64_07795"
     CDS             4279..4665
                     /locus_tag="VHR64_07795"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3302767.1"
                     /translation="MAESSQIAVVWDRIEDVPPFSPAPHIWMRTIAGNQVMMNLVTIE
                     PGGIVPEHSHANEQAGYVLRGTLILTIEGETRHLKTGDCYLAPANVVHSGATTEDGCD
                     VLDVFAPPRPDYVEAAAEARRSSDRH"
     gene            complement(4662..>5431)
                     /locus_tag="VHR64_07800"
     CDS             complement(4662..>5431)
                     /locus_tag="VHR64_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX3302768.1"
                     /translation="PTLTAAARHHAESMATFNYFPADYSVRHEGENQDQTITWQENIA
                     NFGYPDNTHTARSAIIGAGTDSVSTIYRSLTDLPVYREVLTDRRYHAIGIGFGSNSAS
                     EEGSYWAITFGSISDGALAPCDGVAIPVPIVASGRTDNSSDSAVAYDGVYSTYWATTT
                     KKAPPSAWIWFDLGSPQAISEIDWMFAKAGAADTFAIDVSLDRETWSQVTMKSNGAVD
                     EWRSVAWQGTARYIRFYFANPNKDRVLGYLSEVRVLR"
BASE COUNT         1024 a         1759 c         1667 g          981 t
ORIGIN      
        1 cgcctcggcg cggatgtcga catccgccac gtcgacagca atctcgacgg ctacgacgtt
       61 atcgtcgcgc cggcgatcca gatcatcgat caccgccggg ccgagcattt ggcgagcgca
      121 acgcgcaacg cgagcctcgt cgtcggaccc cgcaccggat tccggcagcc gaccgggcag
      181 gtgcatcccg acggccagcc gggaccactg cgtgaactgc tcggctgctc tctgctcaac
      241 ttcgacgcgc tacggccaaa gatgacggtc acggtcgacg acgaggaggt cacgctctgg
      301 gcggagtctt acggctctga gcaggccgag gttatgttcc gatacgacaa cgggccgctc
      361 aagggacagg cagccgtggt gcggaacggc cgcgccttca cgatcggcgc cttcagcccg
      421 atcctgatcg gccgcgtctt cgcccatgtc ctgaccgagg ccggtctcga agtcacacca
      481 ctggatgatg gagtgcgggt cgcccggcgg ggagaccgca cggtctggct caacttcgcc
      541 gaggacgacc accgtctgcc agacggccgc acgatcgacc cagtctcatt cctgatcgat
      601 tgagcggagg gcgttagccg ccgtattcgt cgtcgaggtc ggcgaaatcg cactttgatc
      661 ggaaatcgca aaaccggcaa atcgccggtt cgggtctggc gtgccactcc tgatcgacgg
      721 acagggcgtg aatctgggtc tgtacgatgt tccagatgtg ctcgtttgct gccggcgaat
      781 tgggcacgac ctcgtaggcg cctgagcctg tgagcacgtt gactgttcgc aggcgatcgg
      841 gagtcagact caactgcttg ccgaccgtca accgtgatag atagttctgg atcggatcgc
      901 cgctcagctt tccggtcttg aaatcgatgt ggtcgaccag gccgtcgtca tgccgaatca
      961 cgacatcgac tttggcgcct accgtgacag ctgctccaat cgctctggaa cgaacggaat
     1021 aggcgaactc ttcctcaaca tgcagcactt cggcgttcgc gggaagagcc tgaagcgccc
     1081 gctcgacgtg acccgtgacc actggcgcgt gctcatccct catcggatcg gtcgcaccct
     1141 cggggtactt ctcgaacgag atgtacttcg tcacataagt ctcgatatct gacgatggac
     1201 gatggtcccg acgatcgcga aagatggctg cgagaacctt gtgagtgact cctccgatca
     1261 caagcggcgg cttgaactca aacggcattc gaacgttgcg gatgtagcga aagtggaact
     1321 tgcggggaca tcgatagtac gcgttaatcg actgtgggta gagcttgtac ccgagtcgct
     1381 cgagcgttct ggacggctcc aatctgcgca ctccgccatt ggatcggtta tcaacgattc
     1441 ctaactacaa tctacgccat aaattgaccg gtgcaatact gctttcgtcc tcatggagga
     1501 gatcccatgc cgaatccgcc atcccgtact gatcgcgcgc ggggggtgat cgtcggactt
     1561 gcctgcggcg acgccctcgg cgcgccggtc gaattcgaat cccggcaatc gatcgcggca
     1621 aaatatccgg acggtctgcg cgatttcacg agcggcggct ggatgaacgt cgtccccggc
     1681 gagttgaccg acgacagccg gatgatgatc gacctcgccg agacactggt tcggccgggc
     1741 gcggtcgacc tcgaccacct cgggcagcga ttcgtggcct ggatggcaga aggccccaag
     1801 gacatcggca acaccacccg gatcgcgatc gaggcgctgc gagatggcgt gcactggagc
     1861 gaggcgggcg agctggccct cacacagcgc ggcccgcatg gcgccgccag caatggatcg
     1921 gtcatgcgct gcgccccggt cgcgatccgc ttcgccaaca acccggacca gctccgcgcg
     1981 atctcgatcg attccgcccg gatcacacac gccgagccgc gttgcacctg ggcagcagtc
     2041 gccctgaacc aggcgattgg ccacgcgctc aacggcggag cgatcgacga gcttgtcgac
     2101 gctgcgactt ccggcatcga gcagcccgat gtcgttcgct cgatccagaa tgctccgcac
     2161 cgctcagccc gagatctcaa ggggtcaggg tatgtcctga acgctgtcga gatcgcgttc
     2221 tgggcgacga tcacccgacc gaccttcgaa gaagcagtcg tcagcgcagt catgatcggc
     2281 gacgacaccg ataccaacgc ggccgtgacc ggcgccctcg ccggcgcggt ccatggctac
     2341 ggatcgatac ccgagcgctg gcgatcgacg atctaccaac acgacaggct ggtcactctc
     2401 gccgatcagt tgctctcagc cagctcaagt tggtgagcag caagtgccag gaagcgccgg
     2461 atgctctgac gattgcggac atcgtcggcc aatcccagct catggattag ctcttcctcg
     2521 gtcgcgccgt tgaccgcgcg gccggccgcg aacgtgtctc gcagcgtccg gggcgtgact
     2581 gaacggccga tgcctgcccg cttgcgtacg cgatcgacca taccgttgat cgcctgaaag
     2641 ccgaccggaa aaagccgccc ctgcggatcg cgcgcttcga gcaatgcgcc gtaggcttcg
     2701 gccaatcgct cgctggcgcg gagtgttcgg ttctggttgg acttgcgcgg atcgtccgtg
     2761 acgatgtgaa tgacgaccgc atgcggatcg taccggtcga tgtttccacg ctcgagtttc
     2821 agcacctcgc cgcgggagag tccggcgtcc atcatcagca ggatcgctgt cgggctccag
     2881 ggctcatcgg tcgccgccgc agccaggagc gcctcctgct cgtccgatga gagcggttcg
     2941 gggatcttca gctcgacccg gttcgggtag aagtcctcgg tcgggtcgat cgacagcacc
     3001 atcgcgtcgt cgacaaggaa gcgaaagaag cggcgcacgg aggtcaggcg tcgcttgcgg
     3061 gtcgacacag tattggcctc tccgaggaag cgcatgatgt cgtcggccga aatctggttg
     3121 attgccttgg tcggaatctg gtgcgcgagg acggtcagat cgaagatgta ggattcgatc
     3181 gtgttccgcg gtcgccgctt ccgctcgaga tcagcccgga acgccgccgc ggcggcaggc
     3241 agactcatcc ccggccgcaa tcgctgggcc gtctcctgcc cggaaaaatc gagctgtgcc
     3301 atctgctccc ctccggtcca tgcctaaccc aagggatatg tcgctcacct tatgcgctcg
     3361 ggaccggcaa cgcaagtttc tccgcaaccg tccaccgccg aactcaattg cgctcgccgc
     3421 tatcatgaag atgccgccct aaccagacga caaaggagac gccgcaatgc ctgttcagct
     3481 caccgctacc tatcgggacg cgctcgaata cgcgatcgaa ctccatgccg gccaaacccg
     3541 caagggtacc gacatcccct acatctcgca cgtactcgcc gtcagcgcga ccgtgctgga
     3601 gttcggcggg accgaggagg aagcaatcgc cgccctcctc cacgatgcgg tcgaggacgc
     3661 cggcggtcag gccacccgcg cgcagatcgc cgagcggttc ggcgaagcgg tcgcgcagat
     3721 cgtcgatggc tgctctgacg acagcccgga gatcggcggc cagaagaaac cctggcgtat
     3781 ccgcaagaat gcgcacatcg ctcacctcaa gaaggcgtcg tcatccgtgt cgctggtaac
     3841 cgcggcggac aagctccaca atgcccgcgc gcttgcgagc gacttccgtt acctgggcga
     3901 cgccctctgg gatcggttca acgcttcccg tgaggacatt ctctggtact acggtgcggt
     3961 ggttgacgtc ctcgatgccg gcgatatgcg gctgcatgac cggatgcccg acggaacgac
     4021 gactctcacc gacctcgatc gactgatcga tgaactggaa gttgccgtgg aagacctcat
     4081 cgtcgacgtc gggctcatga cgatgcagtc ggccttcgag gacgcattgc aggaaaacgc
     4141 gctcgacttc ttcggcccaa tcgacatgca ggagcaactc gaacaatcac ggggcagcaa
     4201 ccaaccaccg gcccattgag ccaggaacgc ggtaggcttc ctcatcgata tcttcgactt
     4261 gtggaaggga tgcaggccgt ggctgagtcg tcgcaaatcg cggttgtctg ggatcgaatt
     4321 gaggatgtac cgccgttctc ccctgctccg cacatctgga tgcgaaccat cgccggcaac
     4381 caggtcatga tgaacctggt cacgatcgag cccggcggga tcgttcccga gcatagccac
     4441 gcgaatgagc aagcgggata tgtcctgcgc ggaacactga tcctcaccat cgagggcgag
     4501 acccgccacc tgaagaccgg cgactgctat ctcgctcccg cgaacgtggt ccattccggg
     4561 gcgacaaccg aggacggctg cgacgtgctc gatgtcttcg ccccgccacg ccccgactac
     4621 gtcgaagcgg ccgccgaggc tcgccgctcg tccgaccggc actagcgcag aacgcgcacc
     4681 tcggagagat acccgagcac gcgatccttg ttcgggttcg cgaagtagaa gcggatgtag
     4741 cgcgcggtcc cctgccaggc gacgctgcgc cattcgtcga ccgcgccatt cgacttcatc
     4801 gttacctgtg accacgtttc ccggtcgagc gatacgtcaa tggcgaacgt gtcagccgcg
     4861 cctgccttcg cgaacatcca gtcgatctcg ctgatcgcct gcggcgagcc aagatcgaac
     4921 cagatccagg cactcggcgg cgccttcttc gttgttgtcg cccaatacgt cgaatagaca
     4981 ccgtcgtacg cgacagccga gtcggatgag ttatcggtcc gcccgctggc aacgatcggt
     5041 accggaattg caacgccatc acacggcgcc agcgcgccat cgctgatcga gccgaacgtg
     5101 atcgcccagt aactcccctc ctccgaggcc gagttgctcc cgaatccgat cccgatcgcg
     5161 tgatagcgcc ggtcggtcag cacctcccgg tagacaggca agtcggtcaa tgagcgatag
     5221 atcgtggaca cggaatcggt cccggccccg atgatcgcgc tccgggcggt gtgggtgttg
     5281 tctgggtacc cgaagttggc gatattctcc tgccaggtga tggtctggtc ctggttctcg
     5341 ccctcatggc ggaccgagta atcggccggg aagtagttga aggtggccat gctctcggcg
     5401 tggtggcgcg cagccgcggt caatgtcgga c
//
LOCUS       DATAOZ010000296         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3015165, whole genome shotgun sequence.
ACCESSION   DATAOZ010000296 DATAOZ010000000
VERSION     DATAOZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3015165"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1170
                     /locus_tag="VHR64_07805"
     CDS             16..1170
                     /locus_tag="VHR64_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HEX3302769.1"
                     /translation="MAAAAIRDTQRAIRASRVPRRVLCWEGNSSGFADGFEVLPQRSG
                     TLNDRLAGAFDDLGRDGSARVLLIGMDTPQISAALLETVWDDCDAILGLSEDGGFWAI
                     GLRTPDPARVFAGIEMSTARTGSAQLARLLSLGRSVKLLPPLRDIDEPADAAYVADHF
                     PDLEFSRRHAALIAVPGPDLIFDQLYAGSLVHCDASEERVFRMEAARWSARADDIDQL
                     VVSRCRRPVLDLGCGPGRMVQALNESGVPALGVDTSQVAVQRARSRGALAIKARLAER
                     LPAEGRWGTVLLMDGNVGIDGDVGAVLQRCRALLAPGGTVIVEVDPRPAWHETRRVRL
                     TADAGGYSADLTWTRTGAAAVRRLAGQLDLLVVEERTADGRAFLSLQSAT"
BASE COUNT          251 a          474 c          515 g          275 t
ORIGIN      
        1 ggagaagcag ctgatttggc ggccgcggcc atccgcgaca cccaacgcgc gatccgggcc
       61 tcccgagtcc cccgacgagt tctttgctgg gagggcaatt catcgggctt cgcggacgga
      121 ttcgaagtct tgccccagcg cagcggcacc ctcaacgacc ggctggcggg cgccttcgat
      181 gatcttggtc gagacggctc agcgagggtg ctgttgatcg gcatggatac accgcagatc
      241 agcgctgcgc tgctcgaaac ggtctgggac gactgtgacg cgatcctcgg actgagcgag
      301 gatggcggat tctgggccat cgggctgcgt acccccgacc cggcccgggt tttcgcgggg
      361 atcgagatgt cgactgcacg aaccggctcg gcccagctag cccggctgct gagcttgggt
      421 cgatcggtga agctgctgcc accgttgcgt gacatcgacg agcccgcaga cgccgcatac
      481 gtcgccgatc acttcccaga cttggaattc tcgcggcgcc atgccgcgct gatagccgtg
      541 cctggacccg acttgatctt cgaccagctg tatgccggaa gcctggtgca ttgcgacgct
      601 tccgaggaac gggtgttccg gatggaggct gcgcgctggt ccgcccgcgc cgacgacatc
      661 gatcagttgg tcgtgtcgcg atgccgtcgt ccggtgctgg atctgggctg tggtcctgga
      721 cggatggtgc aggccttgaa cgagtccggc gtaccggctc tcggggtcga cacgtcccag
      781 gtggcggtgc aacgggccag atcccgcggc gctctggcga taaaagcgag gttggccgag
      841 cgattgccgg cagaagggcg ttggggaacc gtcctgctca tggacggcaa tgtcgggatc
      901 gatggcgatg tcggtgccgt gctgcaacgc tgccgagcgc tgctggcccc cggcggcacg
      961 gtcatcgtgg aggttgatcc ccgcccagcc tggcacgaaa ctcggcgcgt ccggttgacc
     1021 gccgacgctg gcgggtacag cgccgatctg acatggacgc gtaccggcgc cgcggcggtg
     1081 cgacgactgg ctgggcaact agacctgctc gtcgtcgagg agcggaccgc tgacgggcgg
     1141 gccttcctct ccctccaatc ggcgacctga gtggccgccg atgtccaagc tctcgcctcg
     1201 aaaaggtggc ccggatatgt tcagcctccc aattccggat gccgtcttgc ggtgacagct
     1261 tggtcgtagc agtagttcga cgaaagggcc tggccatgat cgtttgggac atccccctgt
     1321 tgaagcgcgc cgaaaggagc cccgatggac cagcaagttg agcgattgaa cagcctaaag
     1381 aatctagcga agtacgagga ccagatactc gacgcgattg ccttcacacc ccacggcgcg
     1441 ctgctgtttc tttccgatcc gacccgcttc ctcagcgaag cgggcttcgc ggtcggggaa
     1501 ggtctacagg cagag
//
LOCUS       DATAOZ010000297         6614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9330013, whole genome shotgun sequence.
ACCESSION   DATAOZ010000297 DATAOZ010000000
VERSION     DATAOZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9330013"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1069)
                     /locus_tag="VHR64_07810"
     CDS             complement(77..1069)
                     /locus_tag="VHR64_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302770.1"
                     /translation="MSRKTEAPVMMPSPNGYQHLPGVIPGREPTIILDRVSKWYGDIV
                     AVSDVTFGVTPGVTALLGPNGAGKSTTLKMITGLLSTSTGRISILGKPARGNPGIYRE
                     VGLVHEAEHIYPYLTGREFVRMNAILQRIPGIEQAVDRALEMVEMDYAQDRRIGGYSK
                     GMRQRIKVAAALVHDPDVILMDEPLNGMDPAQRLHMISLIRELGRSKTVMVSSHILAE
                     VERFAENIIVIVNGKLAAAGDYRAIRDRIDTHDHAIRIRSSDPRFLAMTLIGHPSTRA
                     VRVDREDRLVVETQDVRGFYIALPQLAKKADVRLFDVSAADESLASVFSYLVEG"
     gene            complement(1053..1946)
                     /locus_tag="VHR64_07815"
     CDS             complement(1053..1946)
                     /locus_tag="VHR64_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HEX3302771.1"
                     /translation="MAEIDIQGRYGEIYDRGYQHYIGKRLGRRHAIWALITYSMKRAM
                     GIKKSWTAKVIPFILYLAVVGTVVVVIGIEAFLKSAGTVAMSYPDYFSFIYLIEGAFV
                     ATIAPEMLCGDRRENVLALYFSRAISRLDYVLSKLSATAILTMTMSVVPVVFLWLFRQ
                     LLADKPLHALTANIDDLGRVVLIGSLIALFLGCGGLVISSFTGRKSIATAIIFIGYAV
                     LEGFVNALIEAVSSERAKDVLALLSPIRLISELSEKLFGVYDPVASGHGGYPPAVYVG
                     AALGLILLGVAIMVWRYVPED"
     gene            complement(1939..2952)
                     /locus_tag="VHR64_07820"
     CDS             complement(1939..2952)
                     /locus_tag="VHR64_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302772.1"
                     /translation="MVHAQYAPARAGELHPALMPIEGQETGPIISVTNLTKRFGRHTA
                     LSNLTFDVPRACIGLLGANGAGKTTLVRALLGLTNPTNGEATVLGLNTRTNGIEIRKR
                     VGYMPESDCLPPGATAADFVAHMGEMSGLPTQAARQRAADVLYQVGLREERYRLIKGF
                     STGMKQRVKLAQSLVHDPALVFLDEPTAGMDPQGRDDMLELIQRLYRMLGIAVVVSSH
                     ILEDIERVCDHVVIIDQGRLVIDQPLTGMGTPQGDLLVRIDGNQEAFIAQMAARGITV
                     RRPSDDDLQTSDELIVASNAPEVYDAVRDVSAEMGVGLRSLRMKGRSLEDLYLGNVGG
                     GNG"
     gene            complement(3008..4162)
                     /locus_tag="VHR64_07825"
     CDS             complement(3008..4162)
                     /locus_tag="VHR64_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3302773.1"
                     /translation="MSTFALERWMSRWEIEVEFDIAESGVYPLTTRDVINLLPENARS
                     GALDALLDLRLGYTEARGTKALRETLANVYENTTADNILVTTGAIEANYLLFNTLLEP
                     GDHVVAVYPAYQQLYAVAEAIGCEVSRWELRPENNFRYDLDELRRLVRADTKMIVVNT
                     PHNPTGAILSPAELDEVYDLAGSVGAWLLCDEAYRWLDLPGQPPMAPPIRNKGGHGIS
                     VGTMSKPFGLPGLRIGWMAVPAEVAAACWASRDYVSLSPARLSDALAMIAIQHRDAIV
                     IRNQAIAAENLVTATEWFAEHDDLASWTPPRAGLLALLKYNLDVPSQELSDRLAGEYS
                     VMLAPGSAFGFEGYLRIGIGQRPDLFREGLVRTAACLQDLQSSGRRPTEW"
     gene            4539..5888
                     /gene="dnaA"
                     /locus_tag="VHR64_07830"
     CDS             4539..5888
                     /gene="dnaA"
                     /locus_tag="VHR64_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX3302774.1"
                     /translation="MDLPAHQLWQAALSELQGSLSRSSFDNWIRPARLVEVGPDAVTL
                     RADNPQVASTLRTRFADDIGRVLSNLVGRPLEVVVTIDEPDGVAPVVPATRMPEPVAR
                     QLALTANSGLNPRYIFDTYIVGSSNRFAHAASLAVADQPGAQYNPLYIWGGVGLGKTH
                     LLHAIGHRALENNPDLVLTYVSSETFTNEVIASIRNQRMEEFREKYRGVDLLMIDDIQ
                     FIAGKESTQEEFFHTFNALHQSGKQVVISSDKPPKAISNLVERLRSRFEGGLIADVQL
                     PDYEMRIAILSAKAEELGRTLPSDVVEYIAQRDQTNIRELEGALNKVLAYSQIAAKPL
                     NIETAIEALTDATRTARRDSLTPADIAEGVATHFRIALADLRGSSRSQKFVVPRQIAM
                     YLIRELTKASLVDVGEVLGGRDHTTIMHGIEKINREIETDAALRTHITQIREALLTG"
     gene            6144..>6614
                     /locus_tag="VHR64_07835"
     CDS             6144..>6614
                     /locus_tag="VHR64_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302775.1"
                     /translation="MPPLDALGTEYLTLSLEIERIFPGFVDAYMGPDTLKARANSGDL
                     PDPKALLDRAQRLLRDVRISEYDDNRKEFLRAQITGIITSCRKLAGEEIPYVNEVRDS
                     FDISVASVPDADLDASLAELDAALPGDGPVRERNIAYRQQFVVDQPTAARLIDLI"
BASE COUNT         1172 a         2140 c         2168 g         1134 t
ORIGIN      
        1 cggacggcct tgccgccgat gaactcgcgg acgctcagga agaagatcgg cgccatgcgc
       61 cagccgagcg tgcccatcag ccctccacca ggtacgagaa gaccgaggcc agggactcat
      121 cggccgcgct gacatcgaag aggcggacat ccgccttctt tgcgagctgc ggcagcgcga
      181 tgtagaaacc gcgcacatcc tgcgtctcga cgaccagccg atcctcgcgg tcgacccgga
      241 cggctctggt gctcgggtgg ccgatcagcg tcatcgccag gaagcgcgga tcgctggacc
      301 ggatccggat cgcgtggtcg tgcgtgtcga tgcggtcgcg gatggcccgg taatctccgg
      361 ccgcggccag cttgccattg acgatcacga tgatgttctc ggcgaagcgc tcgacctcgg
      421 ccaggatgtg ggacgagacc atcaccgtct tggagcggcc cagctcgcgg atgaggctga
      481 tcatgtgcag ccgctgggcc ggatccatcc cgttcagcgg ctcgtccatc agaatcacgt
      541 cggggtcgtg gaccagcgcc gccgctacct tgatccgctg gcgcatgccc ttggagtagc
      601 cgccgatccg ccggtcctgc gcgtagtcca tctcgaccat ctcgagcgcg cggtcgaccg
      661 cctgctcgat cccgggaatg cgctgcagga tcgcgttcat gcggacgaat tcgcgcccgg
      721 tcaggtacgg gtagatgtgc tccgcctcgt ggaccagacc gacttcgcgg tagatgcccg
      781 ggttgccgcg ggccggtttg ccgaggattg agatccggcc ggtcgaggtc gagagcaggc
      841 cggtgatcat cttgagcgtg gtcgatttgc cggcgccgtt cgggcccagc agcgcggtca
      901 cgcccggcgt cacgccgaag gtcacgtcgg agacggccac gatatcgccg taccacttcg
      961 agacgcggtc gaggatgatc gtcggctccc ggccggggat cacgccgggc aggtgctgat
     1021 atccgttggg tgatggcatc atgaccggcg cctcagtctt ccgggacata tcgccacacc
     1081 atgatcgcga cgccgagcag tatcaaaccg agcgcggcgc cgacgtaaac ggctggggga
     1141 tatccgccgt gcccggacgc caccggatcg tagacgccaa acaacttctc ggagagctcg
     1201 ctgatcagcc ggatcggact cagcagggca agcacgtcct tggctcgctc cgagctgacg
     1261 gcctcgatga gggcgttcac gaatccttcg agcaccgcat agccgatgaa gatgatcgcg
     1321 gtggcgatgc tcttgcgacc cgtgaatgac gagatcacga gaccgccgca gccgaggaag
     1381 agggcgatca gcgagccgat cagcacgacc cggccgagat cgtcgatgtt ggcggtcagg
     1441 gcatggagcg gcttgtccgc cagcagctgc cggaagagcc acaggaacac caccggcacg
     1501 accgacatcg tcatggtcag gatcgccgtc gccgagagct tgctcagcac gtaatccagt
     1561 cgagagattg cccgcgagaa gtagagcgcg aggacatttt cccgccggtc gccgcagagc
     1621 atctccggag cgatcgtcgc gacgaaggcg ccttcgatca ggtagatgaa ggagaagtag
     1681 tccgggtaac tcatcgcgac cgtaccggcg ctcttcagga acgcctcgat accgatcacg
     1741 accacgacgg tgccgaccac cgcaaggtag aggatgaagg gaatgacctt ggcggtccag
     1801 ctcttcttga tccccatcgc ccgcttcatc gagtaggtga tcagggccca gatcgcatgg
     1861 cgccggccga gccgcttgcc gatgtagtgc tgatagccgc ggtcgtagat ctcgccgtag
     1921 cggccctgga tgtcgatctc agccattgcc gcctccgacg ttgcccaggt agaggtcttc
     1981 gagcgagcga cccttcatcc gcagcgagcg caatccgacg cccatttcgg cggagacgtc
     2041 gcggacggcg tcgtagacct ctggcgcgtt gctggcgacg atcaactcgt cactcgtctg
     2101 cagatcgtcg tcggacggcc ggcggaccgt gatgccgcgc gccgccatct gcgcaatgaa
     2161 ggcttcctgg tttccgtcga tccggaccag caggtcgccc tgcggcgtgc ccatcccggt
     2221 cagcggctga tcgatcacga ggcggccctg gtcgatgatc acgacgtggt cgcagacgcg
     2281 ctcgatgtct tcgaggatgt gcgaggagac gacgacggcg atcccgagca tccggtagag
     2341 gcgctggatc agctcgagca tatcgtcccg cccttgcggg tccatcccgg cggtcggctc
     2401 atcgaggaag accagggcag gatcgtggac gagggactga gccagcttga cccgctgctt
     2461 catgccggtt gagaagccct tgatcaggcg gtagcgctcc tcgcgcagcc cgacctggta
     2521 gaggacgtcg gccgcgcgct ggcgagccgc ctgggtcggg agcccgctca tctcgcccat
     2581 atgcgcgacg aagtcggccg cggtcgcgcc gggcgggagg caatcggact ccggcatata
     2641 ccctactcgc ttgcggatct cgatgccgtt ggtgcgggtg ttgagcccga gcacggtcgc
     2701 ttcgccgttg gtcggattgg tcaggccgag cagcgcccgg accagcgtcg tcttgccagc
     2761 gccgttcgcg ccgagcaggc cgatgcaggc gcggggaacg tcgaacgtga ggttgctgag
     2821 ggccgtatga cgcccgaagc gtttggtcag gttggtgacc gagatgatcg ggccggtctc
     2881 ctgaccctcg atgggcatca gcgccgggtg gagctcgccg gcccgcgctg gagcatattg
     2941 ggcgtgaacc acgcgtgaaa tcctgtctcc tggccgggct cgctcaatgc gtcccggtcg
     3001 gaagagtcta ccactcggtc ggtcgtcgcc cgctcgattg caggtcctga agacaggcag
     3061 ccgtgcgaac cagcccctcg cggaacagat ccggtcgttg cccgatgccg atccgcagat
     3121 agccctcgaa gccgaaggcc gagccaggcg cgagcatcac gctgtactcg ccggcgagcc
     3181 ggtccgagag ctcctgcgac gggacatcga ggttgtactt caacaaggcg agcagcccgg
     3241 cgcgcggcgg cgtccacgag gcgaggtcgt catgctcggc gaaccactcg gttgcggtca
     3301 ccaggttctc ggcggcgatt gcctggttgc ggatcacgat cgcatcgcgg tgctggatcg
     3361 cgatcatggc cagcgcgtcg ctgaggcggg ccggcgagag ggagacgtaa tcccgtgagg
     3421 cccagcaggc ggcggcaacc tcggctggga ccgccatcca gccgatgcgg aggcccggaa
     3481 ggccgaacgg cttcgacatc gtgccgacgc tgatcccatg cccgcccttg ttgcggatcg
     3541 ggggagccat cggcggctgc ccgggtagat ccagccagcg gtaggcctcg tcgcagagca
     3601 gccacgctcc gacagagccg gccaggtcgt agacctcgtc caactccgcc gggctgagga
     3661 tcgcgccggt cggattatgc ggcgtgttga ccacaatcat cttcgtgtcc gcccgcacca
     3721 gccggcgtag ctcgtcgagg tcgtagcgga agttgttttc gggcctcagc tcccagcgcg
     3781 agacctcgca gccgatcgcc tcggccacgg catagagctg ctggtaggct ggataaacgg
     3841 cgacgacgtg atcgcccggc tcgagcaggg tgttgaagag caggtagttg gcctcgatcg
     3901 cgccggtcgt aacgaggatg ttgtcggcgg tcgtgttctc gtagacgttg gcgagcgtct
     3961 cacggagggc cttcgtgccg cgggcctcgg tgtagccgag tcgcaggtcg agcagggcgt
     4021 cgagcgcgcc ggagcgggca ttctcgggca ggaggttgat cacgtcgcgc gtggtcagcg
     4081 ggtagacgcc gctctcggcg atgtcgaact cgacctctat ctcccagcgg gacatccagc
     4141 gctcgagcgc aaacgtggac accttcatct cgaggaaccc aatccgatcc gtgaaaccat
     4201 tccgccggtc gctacgtatg aagggtacag aacacagcga tccaaccatc gcacgtgttc
     4261 tcccaagccc aagtggagcg ccgcacacgc tccacctccc cttcccgcat agacccggcc
     4321 agttcggccg ggtctatgta ttgtaggcgc gaacgagacg tcggacttca gacttcagtc
     4381 gtcagattct tgtggatagt ggatagtgat tagtggatag agcggagtcg actactcgcc
     4441 actatgcact atccactaat cacctcaacc tgaagtctga cgtctgacga ctgataatct
     4501 agtcgtaggt atcggcttgg gcttcgaggt acgtgctctt ggatcttcct gcgcatcagc
     4561 tttggcaggc ggcgttgagt gagctgcagg gctcgctctc gcgctcgtcg ttcgataact
     4621 ggattcggcc agcccgcctg gttgaggtcg gaccggatgc ggtcacgttg cgtgcggaca
     4681 atccgcaggt cgcctcgacg ctgcgcactc gctttgccga cgacatcggc cgcgtgctct
     4741 cgaatctcgt cgggcggcca ctcgaggtgg tcgtcacgat cgatgagccg gacggcgtcg
     4801 cgccggtggt gccggccacg cggatgccgg aacctgtggc gcggcaactc gcgctgacgg
     4861 cgaacagcgg cctcaacccg cgctacatct tcgacacgta catcgtcggc tcgtcgaacc
     4921 gcttcgccca tgcggcctcg ctggcggtcg ccgatcagcc cggcgcgcaa tacaacccgc
     4981 tctacatctg gggcggcgtc ggcctcggca agacgcacct gctccacgca atcggtcatc
     5041 gcgcgctcga gaacaacccg gacctcgtcc tgacctacgt gtcgtcggag acattcacga
     5101 acgaggtgat cgcctcgatc cgcaaccagc ggatggagga gttccgcgag aagtaccgcg
     5161 gtgtcgacct gctgatgatc gacgatatcc agttcatcgc cggcaaggag agcacccagg
     5221 aggagttctt ccacaccttc aacgcgctcc accagagcgg caagcaggtc gtcatctcga
     5281 gcgacaagcc gccgaaggcg atctcgaacc tggtcgagcg gttgcgatcg cgcttcgaag
     5341 gcgggttgat cgccgatgtc cagctccccg attacgagat gcggatcgcg atcctgagcg
     5401 ccaaagccga ggaactgggc cggacgctgc ccagcgatgt ggttgagtac atcgcccagc
     5461 gcgaccaaac caacatccgc gagctggagg gagcgctcaa caaggtgctc gcctactccc
     5521 agatcgccgc caagccgctc aacatcgaga ccgcgatcga ggcgctgacc gatgccaccc
     5581 gcacggcccg gcgcgactcg ctgacaccgg ccgacatcgc cgagggcgtc gcgacccatt
     5641 tccggatcgc gctggccgac ctgcgcggct catcgcgctc gcagaagttc gtggtgccgc
     5701 gccagatcgc gatgtacctg atccgcgagc tgaccaaggc gtcgctggtc gacgttggcg
     5761 aggtgctcgg gggacgggat cacacgacga tcatgcacgg cattgagaag atcaaccgcg
     5821 agatcgagac cgatgccgcc ctgagaacgc acatcactca aattcgcgaa gcgttgttga
     5881 ctggttgacc aacccgtagt ggcggggctc cgtacccgcc ttgacctgcc cgaggacccg
     5941 gtcattggag ccggccgcgc catcacggat gtggaacgta ccgtggcatg ccgtcccatg
     6001 tgacccgtgc gatgcctgtg aacggtgcga ttggtgagtc caggcgggta cggagccccg
     6061 ccactacgat tagacggcga ggctggccgg cccgcgctac atttgcggtc gtatctgttg
     6121 cccaggagtt acggagaatc gatttgccgc cgcttgatgc ccttggaacc gagtacctga
     6181 cgctctcgct cgagatcgag cggatctttc ccgggttcgt cgacgcctac atggggccgg
     6241 acacgctcaa ggcccgggcc aactccggcg acctgcccga cccgaaggca ctcctcgacc
     6301 gagcccagcg tctgctgcgg gacgtccgca tctcggaata cgacgacaac cgcaaggagt
     6361 tcttgcgcgc gcaaatcacc gggatcatca cgagctgccg gaagcttgcc ggcgaggaga
     6421 tcccctacgt caacgaggtg cgcgacagtt tcgatatttc cgtagcatcg gtcccggacg
     6481 ccgatctgga cgcctcgctg gccgagctcg acgctgccct gcccggcgat ggtccggtcc
     6541 gggagcgcaa tatcgcctac cggcaacagt tcgtcgtcga tcagccgact gccgctcgac
     6601 taatcgacct gatc
//
LOCUS       DATAOZ010000298         4129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5120173, whole genome shotgun sequence.
ACCESSION   DATAOZ010000298 DATAOZ010000000
VERSION     DATAOZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5120173"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VHR64_07840"
     CDS             <1..437
                     /locus_tag="VHR64_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3302776.1"
                     /translation="HRLSSQMAAEDATSEIFMKAFTGIRGFRNERSFRSWLFAIAHNT
                     VTDNYRAHRPQEPIEAADWLLDPDPSPEERAVLAEGECSVQALLSRVTPQQARILELR
                     LAGLTGPEIAEVLDCSLAAVKIGQVRGYGRLREILAKEGTRS"
     gene            434..1573
                     /locus_tag="VHR64_07845"
     CDS             434..1573
                     /locus_tag="VHR64_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302777.1"
                     /translation="MTEYHDALGHYLDDLLTGAAPITSQLDPELVATIRRLGEIGQTP
                     TPRPDFVDQLEERLMNAVSNVAMVPSPSIRIGPADRASVSPIEPARSVSGARRWLPIL
                     VSAALILVLLGLAWRQLGPGESDRNPQVGAPAIQAPATPSPAEAGDQTLVDVILPADV
                     VPVGEEVNVLLARDTIPANSETTHAPMDCCPGTKTYSILAGSVSVTSDGPMQFIRAGT
                     SGTGEVIAAGDTADLTPGDTLVVRNEHHQTWTSGAGPVDIITTAIASGFWMTSFDPIE
                     WVGNGAASNSQTIPLPAGPYRLQVLGKSISADETVPPTAETIIQLGISSDGTSVGQSS
                     DQTLSVTGVRTPLTVFILRMETTPEGAGTPVAGVSSIQPAASPTP"
     gene            1711..2922
                     /locus_tag="VHR64_07850"
     CDS             1711..2922
                     /locus_tag="VHR64_07850"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX3302778.1"
                     /translation="MSRFIVPQVSLSRRQLLGSAGAVTIAAGMRIPLGRIAAQEASPA
                     ASPPALPAPSSNVSTFAEGLENPRGFRFAADGTLYLAEGGEGGTTSTEGQCEQVVPPV
                     GPYTGGDTARISTIDASGKVTTLADGLASNQTSADLGNLVSGVADVDFVGDHLYALIA
                     GGGCSHGHPDKPNGILRVLADGTTEQVADLSAWVHSNPVATPNPADFEPDEGAFAMAA
                     LDTALYVAESNHGAIDRVDTATGNVTRLIDMTETEGHLVPTALIVGADGNLYVGNLTT
                     FPMVAGAARIWMITPDGQMSRFAEGLTGITGLAFDDQNRLYVLETSGPAAGGAPIVPG
                     TGRVVRLTDSGDLEEIATGLVFPTAMRFGSDGMLYVSNYGFGFPPGAGQIVKIDVSGG
                     PMPSASPSPMS"
     gene            3001..4035
                     /locus_tag="VHR64_07855"
     CDS             3001..4035
                     /locus_tag="VHR64_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302779.1"
                     /translation="MYRLRVLPFAVAFLVVFAAAVPASGGAQEATPVPPRQPAQPTTG
                     PGSSETRFGGMTTIEQKPPDALVADYLLFVPSAPLPGTAQEGEPLPVVIFLHGSGATN
                     ADAYLAWIEHLVWRGAVVLFPLYERPTAAGTLDYQQTVQDDVREGLERLEHDGVPVDL
                     TRVAVVGHSLGAEQALVYAASAATADLPVPTAVMSVAPGCFPMDSEGPCFQVGLGTIP
                     TMTRLLLVSGEDDPGTSGIAQIWAELASVPLENRDVVIPVTDTHGKPRLSADHFQALA
                     GHGAVSGYSSPDAFDWYGTWKWLDALMGCSFDGEWCEYALGNTPEQRYMGAWSDGVPV
                     AQPIVTDEPT"
BASE COUNT          694 a         1417 c         1315 g          703 t
ORIGIN      
        1 accaccgtct ctcctcgcag atggcggccg aagatgcgac tagcgagatc ttcatgaagg
       61 cattcaccgg catccgtggc ttccgaaacg agcgctcgtt ccgaagctgg ctcttcgcaa
      121 tcgcccacaa caccgtcacc gacaactacc gtgcccaccg cccacaggag ccgatcgaag
      181 ccgccgactg gctgctcgat cccgatccct cccccgagga gcgggccgtc ctggccgagg
      241 gcgagtgctc ggtccaggcg ctcctgagcc gggtcacgcc gcagcaggcg cggatcctcg
      301 agcttcgcct ggccgggctg accggccccg agatcgcgga ggtgctcgat tgcagcctgg
      361 ccgcggtcaa gatcggccag gtgcgcggct acggccggct ccgggaaatc ctggcgaagg
      421 agggaacgcg gtcatgaccg aataccacga cgccctcggc cactatctcg atgacctcct
      481 caccggtgcg gcgccgatca caagccaact tgatcccgag ctcgtcgcga cgatccgccg
      541 cttgggcgag atcggccaga cccctacgcc gcgccctgat ttcgtcgatc aactggagga
      601 aaggctcatg aacgccgtat cgaatgtcgc catggttccc tcgccatcga tccgcattgg
      661 tccagccgat agggccagcg tctccccgat cgaacccgcc cgctccgtat caggagcgcg
      721 acggtggctg ccgatcctgg tctcggccgc attgattctg gtgctgctcg gactcgcctg
      781 gcgccagttg ggtcctggcg aatcggatcg caatccgcag gtcggcgcgc cggccatcca
      841 ggctccggcg acgccgtctc ctgccgaagc tggcgatcag acgctcgtcg acgtcattct
      901 gccggccgat gtcgttccgg tgggcgaaga agtcaacgtg ctgctggcgc gggacacgat
      961 tccggccaat tccgaaacca cgcatgcgcc aatggattgc tgtcccggaa ccaagaccta
     1021 ctccatcctc gccggctcgg tctccgtcac gagtgatggg ccgatgcagt tcatccgggc
     1081 agggacctca ggcacaggtg aggtgattgc agccggcgac actgccgatc tcacccccgg
     1141 cgatacgctc gtggtccgta atgagcacca tcagacgtgg acatccggcg caggcccggt
     1201 cgacattatc acgacggcaa tcgccagcgg gttctggatg acatcgttcg acccgatcga
     1261 atgggttggg aatggcgcgg cctccaacag tcaaaccatc ccgctcccgg ccgggccgta
     1321 ccggttgcag gttctgggga agagcatatc ggcggatgag acggtcccac ctacggcaga
     1381 gacgatcatc cagctcggga taagcagcga tgggaccagc gtcggccagt ccagtgatca
     1441 gacgctctcg gtcactggag tgcgcactcc cttgacggtc ttcatcctga gaatggagac
     1501 aaccccagag ggcgccggca cacccgtcgc cggcgtcagc tcaatccaac cagccgcctc
     1561 acctacgccg tagcggccgg ctaggtctac cttgacatgt tgacaacgat cacattcggc
     1621 gtagagtaaa aagtcacgcc aatctcttac ctcttgctgt gctttcgatc ccgtcaggcg
     1681 acgggcgttc ttgtcacaag gaggttcacc gtgtcccgct ttatcgtccc gcaggtatcc
     1741 ctcagtcgtc gccagttgct cggtagtgcc ggcgcggtca cgatcgccgc agggatgcgg
     1801 atcccactcg gtcggatcgc cgcgcaagag gccagcccgg ccgcctcgcc gccggctctc
     1861 ccggcaccct cgagcaacgt ctcgaccttt gcggaggggc tggagaaccc gcgtgggttc
     1921 aggttcgcgg ccgatggcac gctctacctt gccgagggcg gcgaaggcgg gaccacctcc
     1981 accgagggcc agtgcgagca ggtcgtgccg ccggtcggcc cgtacaccgg tggcgacacg
     2041 gcccgcatct cgacgatcga tgccagcggc aaagtcacga cgctcgctga tggcctggcc
     2101 tcgaaccaga cctccgcgga tctcggcaac cttgtcagtg gcgtcgccga cgtggacttc
     2161 gtgggtgacc atctctatgc cctgatcgcc ggcggcggtt gctcccacgg gcacccggac
     2221 aagccgaacg gcatcctcag ggtccttgct gacggcacca cagagcaggt cgcagatctg
     2281 agcgcctggg ttcattccaa cccggtggcg acgcccaacc cggccgactt cgagccggat
     2341 gagggcgcct ttgcgatggc cgcgctcgac accgcactct acgttgccga gtcgaaccac
     2401 ggtgcgatcg accgggtcga caccgcgacc ggcaatgtca ctcgcctgat cgacatgacc
     2461 gaaaccgaag gccacctcgt cccgactgcg ctcatcgtcg gcgcggacgg caacctctac
     2521 gtcggcaacc tgacgacatt cccgatggtg gccggggctg cccgcatctg gatgatcacc
     2581 ccggacggcc aaatgtcgcg gttcgccgag ggactgaccg ggataaccgg cctcgccttc
     2641 gacgaccaga atcgcctcta cgtgctcgag acgtcgggtc ccgccgccgg tggcgccccg
     2701 atcgtgcccg ggaccggccg cgtcgtgcgg ctgaccgact cgggcgatct ggaggagatc
     2761 gcgaccggcc tggtcttccc gacggcgatg cggttcggct cggacggcat gctctacgtc
     2821 tcgaactacg gcttcggctt tccgcccggc gccggccaga tcgtcaagat tgacgtctca
     2881 ggaggcccga tgccatccgc atcgccgtcg ccgatgtcct gagcgatggg ataacgcggc
     2941 ctgctttgcc agtagaccgg tcgagttgaa gagccgttcc ttgtgcagga ggagtccggc
     3001 atgtaccggt tgcgcgtcct cccattcgcg gttgcgttcc tggtcgtgtt tgctgcggcg
     3061 gttcccgcat caggcggagc acaggaggct accccggtcc caccgcggca accggcgcag
     3121 ccgaccactg gccccggcag cagcgagaca cgctttggcg ggatgaccac tatcgagcag
     3181 aagccgcctg atgcgctcgt tgccgactat ttgctgttcg tgccctccgc cccgctgccg
     3241 gggacggcgc aggaggggga gccattaccc gtggtcatct tcctgcatgg ctcaggggcc
     3301 accaatgccg atgcgtacct ggcctggatc gagcatctgg tctggcgcgg tgcggtcgtg
     3361 ctctttccac tctacgagcg cccaactgct gcggggacac tcgattacca gcaaacggtc
     3421 caggatgacg tgcgcgaggg tctggaaagg ctggagcacg acggtgtccc ggttgacctg
     3481 acccgggtgg cggtcgtcgg ccactcgctc ggcgccgagc aagcgctggt ctacgcggcg
     3541 agcgccgcga ccgccgacct ccccgtcccg acggcggtga tgagcgttgc tcccggctgc
     3601 ttcccgatgg acagcgaagg cccctgcttc caggtaggcc tgggcaccat cccgacgatg
     3661 acgcgcctcc tgctggtgag cggggaagat gaccccggga cgtccggaat tgcgcagatc
     3721 tgggccgagc ttgcgagtgt gccgctggaa aaccgggatg tggtgatacc ggtcacggat
     3781 acgcatggga aaccccggtt gtctgccgat cacttccagg ccctcgcggg ccatggcgct
     3841 gtctcggggt acagctcgcc ggatgccttc gactggtacg gcacctggaa gtggctcgat
     3901 gccctcatgg gctgctcctt cgatggcgag tggtgcgagt acgccctcgg caacacgccg
     3961 gagcaacgct acatgggcgc gtggagcgac ggggtcccgg tcgcgcagcc aatcgtcacc
     4021 gacgagccga catagcgccc ggcgtttcgg ggccgggatg cgacccggca agcggcgacg
     4081 agaccgcgcc cgacacctgc tttgccccaa tagcatcgac caatcgctc
//
LOCUS       DATAOZ010000299         4315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3806392, whole genome shotgun sequence.
ACCESSION   DATAOZ010000299 DATAOZ010000000
VERSION     DATAOZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3806392"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VHR64_07860"
     CDS             <1..1002
                     /locus_tag="VHR64_07860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3302780.1"
                     /translation="ENGGLGLGVTEASIVLEEINRSGGSSAACHAQLYLLSALVRHGS
                     DEQKHRWLPPIAAGASRLQAFSVTEPEAGSDTTAIRTTAVRDGNSFVVNGHKNWTSRI
                     EQSDLLLLLARTTAREEVADRDRGISLFLVDLREVRLRQPEAIEVHPVRVMFNYETFQ
                     VTYRDLRIPADALIGQEGTGFRSVIDGWNAERILLAAECIGDGDWFVDKASRYAGERS
                     VFGRPIGQNQGVQFPISRAYAQIRAADLLRYQAAWLYDRGEPCGAEANMAKLLSSDAS
                     WAAANACLDTHGGNGFVAEHDVERKFRETRLFQVAPINNNLVLAYIAHHVLGMAKSY"
     gene            1216..1941
                     /locus_tag="VHR64_07865"
     CDS             1216..1941
                     /locus_tag="VHR64_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302781.1"
                     /translation="MSEDRTTLDASDPAAIAEHHPTPQIADYQHSPRSRIPSWANEAV
                     DVLLVVVLALAAVAAAWSGYQAARWSGVQASNYAQASAKRIESTRASTTANQETTVDV
                     ITFSNYVNAWRAGDTALADFYRKRFRLEFVPAFDAWMATDPVNNPDAPPSPFSMPEYA
                     PANLTLATQLQAESEQLYATGTDANQRADDYVLNTVFLATVLFFAGLAPRIQWLPAQL
                     VLVVLAVGLLGFGLFKVVTYPIE"
     gene            complement(1964..3121)
                     /locus_tag="VHR64_07870"
     CDS             complement(1964..3121)
                     /locus_tag="VHR64_07870"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX3302782.1"
                     /translation="MLADPSAFLRGAAVIMAYDLVTTPTIGLCGHICGDAHLNNFGAY
                     ATPERALVFDLNDFDETNVGPWEWDVKRLAASIVVAGRVNGLSDSDCRDAALRAAASY
                     RSRIRSFANMRYLDVWYCSIEATEITNHLNDDDIAGQFISTAQKNTHLGTLPKLVEVG
                     SSGPRIKNDPPLVEHRTYPLAQRISDVWNGYRSSLTEERRVILDRYQLVDVARKVVGV
                     GSVGLRCFVMLLLGRDDKDPLFLQVKEARPSALSPSIRESRFGNEGKRVVRGQRIMQA
                     ASDLFLGWTRCGRIDYYVRQLRDMKASVPTGELDKADLAVYAEFCGWALARAHACSMD
                     AAPIGGYLGSGDVFDHAISEFAVAYADQTERDYAALVKAVKSGQVAAETGI"
     gene            complement(3079..3264)
                     /locus_tag="VHR64_07875"
     CDS             complement(3079..3264)
                     /locus_tag="VHR64_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302783.1"
                     /translation="MASPSGSASVDPATLIGSHHLIAPIRLRSFARPTAADYRVSYQS
                     GTAECSPTRRRFFAALR"
     gene            complement(3566..>4315)
                     /locus_tag="VHR64_07880"
     CDS             complement(3566..>4315)
                     /locus_tag="VHR64_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302784.1"
                     /translation="LVPAAILLDWPRIRAWWTRWMSLPHALHQPSLVECTMRHTSALL
                     DVAAIGALLTFVFVGGLLHPDFTDYWGYMVAQMAGTILAVMWLTQQFSAEGGLSWLTH
                     VIIVAVTYADTLGTAAGFYDRYQVYDKITHFGGGAILAAVAYEIALALRLRGAITWDL
                     QRRMIVAIGVSLTLGAIWEFYEVFGDAIFDTGRHAGSRDTIYDLISDFSGAILAVALL
                     ARFEPNRVEVSPFKRASRSDGMTIGRTGEPA"
BASE COUNT          849 a         1399 c         1320 g          747 t
ORIGIN      
        1 gagaacggcg gactagggct cggcgtgacc gaagcgagca ttgtcctcga ggagatcaac
       61 cgctccggcg gttcgtcggc cgcctgtcac gcccagctct atctcctcag tgcgctcgtc
      121 cggcacggca gcgacgagca gaaacaccgc tggctgccgc ccattgcggc gggtgcatca
      181 cggctgcagg ctttttcggt caccgaacct gaggccggtt ccgataccac cgcgatccga
      241 accacggcgg tccgcgacgg caactccttc gtggtcaatg gccacaagaa ctggacgagc
      301 cgaatcgagc agtcggacct gctgctgctc ctggcccgga ccacagcgcg ggaggaggtc
      361 gccgaccgcg accgcggcat cagcctcttc ctggtcgacc tgcgcgaggt gcggttgcgc
      421 caaccggagg cgatcgaagt gcaccccgtc cgggtcatgt tcaactacga gaccttccag
      481 gtaacctacc gagacctgcg catcccggcc gacgcgctga ttggccagga gggaacggga
      541 tttcgctccg tgatcgatgg ctggaacgcg gagcggatcc tgctcgcggc cgaatgcatc
      601 ggcgacggcg actggttcgt cgacaaggcg tcccggtacg ccggcgagcg gagcgtcttc
      661 ggccgcccga tcggacagaa ccagggggtc cagttcccca tctcccgcgc ctacgcccag
      721 atccgtgctg ccgacctgct gcgctaccag gcggcctggc tctacgaccg cggcgaacca
      781 tgcggcgccg aggcgaatat ggccaagctg ctgtcgagcg acgcgagctg ggcggccgcc
      841 aacgcctgtc ttgacaccca cggcggcaac ggcttcgttg ccgagcacga tgtggagcgc
      901 aaattccggg agacgcgcct cttccaggtc gctccgatca acaacaactt ggtcctggcc
      961 tatattgccc accacgtcct cggcatggcg aagtcgtatt gatgttcggc gcgcaaccgc
     1021 gccctgcgga accgaggcat cgaaaacggt tgtcgaatca ggttcatgcg ccgaatacgc
     1081 ccttcggcgg atgacggtcc gcccagccgt cgctacggtg agtcggcacg ctaccgctga
     1141 atcggtggcc gggcgcacct cgtgatgctc gtggccgacc accgtcacca cgtcgcactg
     1201 aaggagggcg cggtaatgtc ggaggaccgg accactctgg atgcctcaga tccggctgcc
     1261 atcgccgagc atcacccgac tccccagatc gcggattacc aacactcccc aaggtctcgc
     1321 atcccgtcgt gggcgaacga ggccgtcgac gtgctcctcg tggtcgtgct ggcattggca
     1381 gctgttgccg cggcctggag tggctatcag gcggcgcgct ggagcggtgt tcaggcgtcg
     1441 aattacgcgc aagcctccgc taagcggatc gagtcgacgc gcgccagcac gaccgcaaac
     1501 caggagacga ccgttgatgt gatcacgttc tccaactatg tcaatgcctg gagagccggc
     1561 gacacggcgt tggcggactt ctaccgcaaa cggtttcggc tcgagtttgt gcccgccttc
     1621 gatgcctgga tggcgactga cccggtgaac aacccggacg cgccgccatc gccattctcg
     1681 atgccagaat acgcgcccgc caacttgacg ttggcgaccc agcttcaggc ggaatcggag
     1741 caactgtatg ccaccgggac ggatgcgaac caacgggcag acgactacgt actcaacacg
     1801 gtgttcctgg cgacggtgct attcttcgcg ggccttgccc cgcgcatcca gtggctcccg
     1861 gcccaattgg tgctcgttgt cttggctgtc ggcctgctcg gattcgggct cttcaaggtc
     1921 gtcacctacc caatcgaatg accaccgccc agccagaatg tcgtcagatg cccgtttcgg
     1981 cggcaacctg tcccgatttg accgccttca cgagtgcggc gtaatcgcgc tcggtctgat
     2041 cagcgtacgc aaccgcgaat tccgagatcg catggtcaaa aacatcgccc gagccgaggt
     2101 aaccgccgat cggagcggca tccatcgagc acgcgtgcgc gcgagcaagg gcccaaccgc
     2161 agaactcggc gtagacggcc agatctgcct tatccagctc accggtcggg accgaagcct
     2221 tcatgtcacg aagctgacgc acgtagtaat cgattctccc gcatcgggtc cagccgagaa
     2281 agagatcgct cgcggcctgc atgatgcgct gaccacgaac aacgcgcttc ccctcgttgc
     2341 cgaatcttga ttccctgatg gaaggcgata gagcagacgg ccgtgcttcc ttgacttgca
     2401 gaaagagtgg atccttgtcg tcacgcccga ggagcaacat cacgaagcaa cggagaccga
     2461 cgctcccgac cccaacgacc ttgcgcgcaa cgtcgacgag ctggtaacga tccaatatga
     2521 ctcgtcgctc ctctgtgagc gagctgcggt aaccattcca gacgtccgag atgcgctgcg
     2581 ccagcggata cgtgcgatgt tcgaccaggg gtggatcatt cttgatccgt gggcccgaag
     2641 acccgacctc gacgagcttg ggcagcgtgc caagatgcgt gtttttctga gcagtggaaa
     2701 taaactggcc cgcgatatcg tcatcgttga gatgattcgt gatctcggtt gcctcgatcg
     2761 agcaatacca gacgtcgagg tagcgcatgt ttgcgaacga ccggatgcgc gaccgatacg
     2821 aagccgcggc cctgagagcc gcatcccggc agtccgaatc agacagtcca ttgacccgcc
     2881 ccgcgacgac gatgctggcc gcgaggcgct tcacatccca ctcccacgga ccaacatttg
     2941 tctcgtcgaa gtcattgaga tcgaaaacta gtgcgcgttc cggcgtcgcg taggcgccga
     3001 agttgttgag gtgcgcgtcg ccgcagatgt gcccgcacag cccaatcgtg ggcgtcgtca
     3061 cgagatcata ggccatgatc accgcagcgc cgcgaagaaa cgccgacggg tcggcgagca
     3121 ttcggccgta ccggactggt acgagacgcg gtaatcggcc gcggtcggtc tggcgaagga
     3181 gcgcaatcgg atcggggcga tcaggtggtg gctgccaatc agcgtggctg gatcgaccga
     3241 cgcgctccct gaaggacttg ccatcgtcgc ggcctcgccc gccagtttgc cggttgacaa
     3301 tcatctctga aggatggact tgtgaagtca catcgattcc cttacggcgg ggtctgaccg
     3361 caaagctggt gacctcagtc tctggtgggc tgccggttgc gtcatctcgt gagccaacag
     3421 ggtccgggag gacgcctgaa gtagcgccga acccggaatc gcgccgacct attccgaccg
     3481 ttctaccgca tccgcacaaa ccggaaagtc gtcatgcaac aaacgattca cggcttcatg
     3541 attcaggccc cgcacatgcc taatttcagg cgggttcacc ggtcctcccg atcgtcatcc
     3601 catcgctcct ggacgcacgc ttgaacggag agacctcaac gcgattgggc tcaaaccgcg
     3661 cgagcagggc aaccgccagg atcgcacccg agaaatcgga gatgaggtcg tagatcgtgt
     3721 cccggctccc tgcatgccga ccggtatcga agattgcatc gccgaacacc tcgtagaact
     3781 cccagatcgc gcccagcgtc agcgagacac cgatcgcgac gatcattcgg cgctggaggt
     3841 cccacgtgat cgccccacgc agccgcaacg ccagtgcgat ttcataggcg actgcggcta
     3901 gaatggcgcc gccgccgaag tgggtgatct tgtcgtagac ctggtagcga tcgtagaagc
     3961 cggcggcagt gccaagcgtg tcggcatacg tcacggcaac gatgatcacg tgggtcaacc
     4021 aggacagccc gccctccgcg gagaattgct gggtcagcca catgaccgcc agaatggtcc
     4081 cggccatttg cgccaccatg tatccccagt agtcggtgaa atcgggatgg agcaaaccgc
     4141 ccacgaagac aaaggtgagc aaggccccga tcgccgccac atccagcagc gccgaggtgt
     4201 gtcgcatagt gcattcgacc aggcttggct ggtgcaacgc atggggaagg gacatccacc
     4261 gggtccacca ggcgcggatc cgtggccagt caagcaatat tgcggctggc acaag
//
LOCUS       DATAOZ010000300         6505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_386369, whole genome shotgun sequence.
ACCESSION   DATAOZ010000300 DATAOZ010000000
VERSION     DATAOZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386369"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VHR64_07885"
     CDS             complement(<1..510)
                     /locus_tag="VHR64_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302785.1"
                     /translation="MSTREPSNETRDGTGFSRRAALMRGGAIGTAAAVLGSLGLRQRT
                     LAQDATPMAAPAGWRTHHVEVTFAPHDPVSITLAGSGPPQRGDHFYIDAPIYAKGDEA
                     GSEIGIYRCFGAWVAAADATDAPAQRLTTVQYVLFNDGIIFGLINEGGANPDMHVGAV
                     QGGNGTYTGA"
     gene            complement(561..1262)
                     /locus_tag="VHR64_07890"
     CDS             complement(561..1262)
                     /locus_tag="VHR64_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302786.1"
                     /translation="MQAQIGRRSVFRRGALGLTGAALAVRVSAPAAHAKGELMTASGA
                     GVNLKLMPDPDGKPVVPMRESFAFDAIYAQCIVEDNAERFAMDTFGMGRVVIEPHQFF
                     MAMYADTVGLDRITQEANGARTATMVGSIFCSTFAGTATVAVGSREAQEHARFEITAT
                     DGGSGAGAPPDSFAFTVFFDPKDAPVNHGVFGPKFTFTGEMIAGKITIGPPIGVEPTE
                     ATPTANRSPGRGTYY"
     gene            complement(1299..1835)
                     /locus_tag="VHR64_07895"
     CDS             complement(1299..1835)
                     /locus_tag="VHR64_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3302787.1"
                     /translation="MDTKQSRFGAASHSDGITRREAVLGGTGGLALALFALGATQARA
                     QEATPSPAGGMPAGVGVLPGVDVNVEDMPAAPVKVSIYHLTLEAGTNIPPSSAPYPEI
                     AFAEKGTLICPGAAGRYLIKSDGTSMEVGDEDVTVNEGDALYTPANALDGARNDSSEQ
                     ITVLIIDFLAGGEATPTS"
     gene            2027..2503
                     /locus_tag="VHR64_07900"
     CDS             2027..2503
                     /locus_tag="VHR64_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3302788.1"
                     /translation="MASRAQMQFTIRPMSDDSARDISGWRYPPPYDFYDAIPDDPDLA
                     DLLSADFRRGRYFEVVDITGDLVGFFEFKHEVTPLEIGLGLRPDLTGHSLGLTFVQAG
                     MDFARQELGATTLSLAVATFNIRAKTVYERAGFRPVETYLHRINGIDHEFLRMRTD"
     gene            2583..3347
                     /locus_tag="VHR64_07905"
     CDS             2583..3347
                     /locus_tag="VHR64_07905"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3302789.1"
                     /translation="MALRDLTALRPDRLPFAPFTRCDEAELMDLPDQDEVELAENLRD
                     IRRVNRLLGGTAIILRHLPRLIASIPPTQAISVLDLATGSGDIPLAISRWAKRHGTAM
                     TIVASDLSEPILREARKQLANHPKITLARYDARAVPLPDKSVDIALCSLSLHHFRPDD
                     AVHVLREMDRIARHGFILNDLRRGRIGYAAAWLAAHITTRNRLTRNDAPLSIRRAYTP
                     DELADLLQRAGVAGARISTHLWFRMAAVKTTPNAHA"
     gene            3340..4548
                     /locus_tag="VHR64_07910"
     CDS             3340..4548
                     /locus_tag="VHR64_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX3302790.1"
                     /translation="MRETDAEAIVVGGGPAGSTIAAALATAGHRVLLLDKASFPRHKP
                     CSDYLNAGGRTILAGMGVLDEVMRAGAREINGMVVHAPNGNRFTADFAMAEPGQCALG
                     LSRRCLDQLLLDRARAAGVTVREHAHVRDLIRDGDRVAGVEATIDGVSEQIRAPLVIG
                     ADGRYSVVARTLDLTTPLRWPRKTGLAAHYRGVTGLDGYGEMHVGHGVYAGLAPIEHG
                     MANLTMVLPDRSVEDRAGSVDELLADALGRLPALAQRLDGVERVGTIRGVGSMGQRAR
                     RTTGDGFLLIGDAASFLDPFPGEGIYEALKAALLAAPVASAALRFGDTSAVALGPYRI
                     ARRHAFTAKRQVCWIVQGFVNAPPLMNYVTDRLARRDDLGMTLSGVLGNLRPATQALS
                     PLYLARLLRP"
     gene            4559..5041
                     /locus_tag="VHR64_07915"
     CDS             4559..5041
                     /locus_tag="VHR64_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3302791.1"
                     /translation="MRSANTIEMAGDLDRIVALAADIERWPVILPHYRWVTLLAGGGD
                     HKTVEMAARRGRIPVKWRAIQEIERDDNPPIIWFTHIGGVTKGMEVAWTFDCTPGTVT
                     VRIDHVFTPPWPLIGNGVAERIIGPHFVEAIAGKTLATIKAIVEDRDPRFDPDGRPRS
                     "
     gene            5038..6291
                     /locus_tag="VHR64_07920"
     CDS             5038..6291
                     /locus_tag="VHR64_07920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX3302792.1"
                     /translation="MKQRVAITGIGAITPIGTGVEELWAGVRCGRPAIRTIDRFDPAQ
                     TASKMAGQIEFDPLRYMTPKRARRLDRYSQFALVSAQMALDDAGLSAEEAGCAGAGIY
                     VGSALGGVAFAEEQHEGYVRNGPHGVNPLLALSVFGGAASSNVTIELGLTGPSLAYGN
                     SCAAGLIAIGEAARLIEDGRVPVMLAGGAEAPLSPLVVGAFAMIRVLSTRNDDPSTAS
                     RPFDKGRDGFVIAEGSAVLVLEEFDHAKTRGARIYAEIPGYATTSDAYHMTAPHPDGT
                     HAARAMCAAMRSAGLSPSAIDYVNAHGSSTVLNDPTECRAIRTALGRYAERVAVSGTK
                     GLHGHALGATGAIEAAISALALQRGHLPGTANLVDQDPACDLDIIPPGGRDQQIGHLL
                     CNAFGFGGVNATLAMSCSGRGSIEH"
BASE COUNT         1057 a         2230 c         2117 g         1101 t
ORIGIN      
        1 cgcgccagtg tacgtgccat tcccgccctg aacggctccg acgtgcatgt ctggattggc
       61 accgccctca ttgatcagac caaagatgat gccgtcgttg aaaaggacgt actgcaccgt
      121 ggtcagtcgc tgcgccgggg cgtccgtcgc atctgctgcg gcaacccacg cgccgaagca
      181 ccggtagatc ccgatctcgg atccggcctc gtcgcctttg gcataaatcg gggcatcgat
      241 gtaaaagtgg tctccccgtt gcggcggccc gctgccagcg agcgtgatgc ttacggggtc
      301 atgaggggcg aacgtgacct cgacatggtg ggtacgccat ccggcgggcg cggccatcgg
      361 tgtcgcatcc tgggccaacg tacgctggcg gagtccgagc gatcccagca cggctgcggc
      421 agtgccgatg gcgccgcccc gcatgagcgc ggccctgcgg gaaaatccgg tgccatctcg
      481 agtctcattt gacggttctc gggtgctcat ctcatttcct ccatcgctga caacatcgaa
      541 gatgcccgaa ctgagaaccc tcagtagtag gtgccgcggc cgggcgaccg atttgccgtc
      601 ggggtcgcct cggttggctc gacgccaatc ggcgggccga tcgtgatctt gccggcgatc
      661 atctcgccgg tgaaggtgaa cttcggtcca aagacgccgt gattgaccgg cgcatccttc
      721 ggatcgaaga aaacggtgaa agcgaaactg tccggtggcg cgcccgcgcc ggagccgccg
      781 tcggtcgccg tgatctcgaa gcgggcatgc tcctgcgcct cgcgcgatcc gaccgcgacg
      841 gtggcggtac cggcaaaggt cgagcagaag atcgagccga ccatcgtggc cgttcgcgcg
      901 ccgtttgctt cctgggtgat ccggtcgagg ccgaccgtat cggcgtacat cgccatgaag
      961 aactgatgcg gctcgatcac gacccgcccc atcccgaagg tatccatcgc gaagcgctcg
     1021 gcgttgtctt cgacgatgca ttgggcgtaa atggcgtcga acgcgaacga ctcgcgcatc
     1081 ggcacgaccg gcttcccgtc agggtccggc atcagcttga ggttgacgcc cgccccggag
     1141 gcggtcatca gctcgccttt ggcatgagcc gccggtgctg atacccggac ggcgagtgcc
     1201 gctccagtca gacccagggc tcctcgtcga aatactgagc gtcggccaat ctgcgcctgc
     1261 atccgcattt cctcccggaa attccatgag gctcgacgtc aggacgtggg tgtcgcctca
     1321 ccgcctgcca ggaaatcgat gatgagaacg gtgatctgtt ccgagctgtc attccgggcg
     1381 ccatccaggg cattcgccgg ggtgtagagc gcatcgccct cgttgactgt cacgtcctca
     1441 tccccgactt ccatcgaggt gccgtccgac ttgatcaggt atcgaccggc ggcgcccggg
     1501 cagatgagcg ttcccttctc ggcaaaggca atttccgggt acggagcgga cgagggcggg
     1561 atattcgtgc cggcttcgag ggtgaggtga tagatggaga ccttcaccgg tgcggctggc
     1621 atatcctcga cgttcacatc gacgccgggc agaacaccga cccccgcggg catgccgccg
     1681 gccggggaag gtgtcgcttc ctgggcccgg gcctgcgttg ctccaagcgc aaacagtgcc
     1741 agggccaggc ctcccgtccc tccgagcacc gcctcgcggc gggtgatccc atccgaatgc
     1801 gaggccgcgc cgaatctcga ctgcttcgtg tccatgccga ctccttcctt gaaagaggtt
     1861 cagtcggaac gccgcagaat gtgattgtgg ttcgctttgc aaaatatcac gcaatgacgc
     1921 catgtcaacc ccgttcagac gcctccgcca gccgaaaacc tgcatggcgg tggccatgct
     1981 gactgcgtac ccttgtgcag tagcgatgcg aacagaggga cttggcatgg cttcgagagc
     2041 gcagatgcag ttcaccatcc ggcccatgag cgacgattcg gcccgtgaca tctccggctg
     2101 gagatacccg ccgccctacg acttctacga cgccatcccg gacgatcccg acctcgccga
     2161 tctcctcagc gcggacttcc gtcgcggccg gtacttcgag gtcgtcgaca ttaccggcga
     2221 cctggtcggc ttcttcgagt tcaagcacga ggtcacgccg ctcgagatcg ggctcggcct
     2281 gcgaccagac ctgaccgggc acagtctcgg tctcaccttt gtgcaggctg ggatggactt
     2341 cgcgcggcag gagctcggcg caaccacgct ctcgctcgcc gtcgcgacct tcaacatccg
     2401 cgcgaagacg gtctacgagc gcgcaggctt ccgcccggtc gagacttacc tccaccgcat
     2461 caacggcatc gaccacgaat tcctgcgcat gcggaccgac tagacccggc gtgtcgcctg
     2521 cgttacgcaa ttagggcccg ctgacgggta cgctgactgc catccgaagg agcatgcacc
     2581 ggatggccct cagagacctg accgcgcttc gtcccgatcg acttccgttc gcaccgttca
     2641 cccggtgcga cgaagccgag ttgatggact tgcccgatca ggacgaggtg gagctggctg
     2701 agaacctgcg cgatatccgc cgggtcaatc gcctgctcgg cggcacggcc atcatcctgc
     2761 gccatttgcc acgcctcatc gcatcgatcc cgccgaccca ggcaatctcg gtgcttgatc
     2821 tcgcgaccgg atccggcgac attccgctgg cgatctcgcg ctgggccaag cggcacggta
     2881 cggcgatgac gatcgtggcc agcgacctct ccgagccgat cctgcgtgag gcccgcaaac
     2941 aactcgccaa tcatcccaag atcacgctcg ccagatacga cgctcgcgcc gttcccttac
     3001 cagacaagtc ggtcgatatc gcgctttgtt ccctctcgct tcaccacttc cgacccgatg
     3061 acgcggtgca cgtgctccgc gaaatggacc ggatcgcccg gcatggcttc atcctcaacg
     3121 acctccgccg gggccggatc gggtacgccg cggcctggct cgcggcccac atcacgacgc
     3181 gcaatcgcct gacccgcaac gacgcgccgc tttcgatccg ccgagcctac acaccggatg
     3241 agctggccga tctcctgcag cgggcgggtg tcgcgggagc ccggatctcg acgcacctct
     3301 ggttccggat ggcggcagtc aagaccacgc cgaatgctca tgcgtgagac ggacgccgag
     3361 gcgatcgtgg tcggcggcgg gccggccggc agcacgatag ccgcggcgct ggccacggcc
     3421 gggcaccgcg tgctgctgct cgacaaggcg agcttcccgc gccacaagcc ctgctccgat
     3481 tacctgaacg ccggcggccg cacgatcctg gccgggatgg gcgtgctcga cgaggtcatg
     3541 cgggccgggg cgcgcgagat caacgggatg gtcgtccatg cgccgaatgg caaccgcttc
     3601 acggcagatt tcgcaatggc tgagccaggg caatgcgcgc tcggtctctc ccgccgatgc
     3661 ctcgatcagc tcctgctcga ccgcgccagg gcagccggcg tgacggtccg cgaacatgca
     3721 cacgtccgcg acctgatccg cgatggcgac cgggtcgccg gggtcgaggc gacgatcgat
     3781 ggcgtctccg agcagatccg ggcgccgctg gtcataggcg cggacggtcg atactcggtc
     3841 gttgcgcgca cgctggacct gaccacgcca ctccgctggc cgcgcaagac cggactcgcc
     3901 gcccactacc gcggcgtgac cgggctcgat gggtacggcg agatgcacgt cgggcacggg
     3961 gtctacgcgg gcctggcgcc gatcgagcac ggcatggcga acctcacgat ggtgctgcct
     4021 gatcgctcgg ttgaagatcg ggccggctcc gtcgacgagc tcttggccga cgccctcggc
     4081 agattaccgg ccctggccca gagattggac ggagttgagc gagtcggcac gatccgcggc
     4141 gtcgggtcga tgggccagcg cgcgcggcgg acgaccggcg acgggttcct gctgatcggc
     4201 gacgctgctt cgttcctcga cccattcccg ggcgagggca tctacgaggc gctgaaggcc
     4261 gccctgctgg ccgctccggt cgccagtgcc gccctgcgtt tcggagacac gtcggcagtc
     4321 gcactcgggc cctaccggat cgcgcggcgc cacgcgttca cggctaagcg gcaggtctgc
     4381 tggatcgtcc agggcttcgt gaacgcgccg ccgctgatga actacgtgac cgaccgcctt
     4441 gcccgccggg acgatctcgg catgaccctg agtggcgtgc tcggcaatct tcggccggct
     4501 acgcaggcgc tttcgccgct ctaccttgcc cgcctgctcc gcccatgagg agtgcctgat
     4561 gcgcagcgcg aacaccatcg agatggccgg ggacctcgat cgcatcgtcg cactcgcggc
     4621 cgacatcgag cgctggccgg tcatcctgcc ccactaccgc tgggtgacgc tgctcgccgg
     4681 cggaggcgac cacaagacag tcgagatggc cgcacggcgg ggacgaatcc cggtcaagtg
     4741 gcgggcgatc caggagatcg agcgggacga caacccgccg atcatctggt ttacgcacat
     4801 cggcggcgtc acgaagggga tggaggttgc ctggaccttc gactgcacac ccggcacggt
     4861 caccgtccgc atcgaccacg ttttcacgcc gccgtggccg ctgatcggca acggcgttgc
     4921 ggagcgcatc atcgggccgc acttcgtcga agcaatcgcc ggcaagacgc tcgccacgat
     4981 caaggcgatc gtcgaggacc gggatccccg cttcgacccg gacgggcggc cgcgatcatg
     5041 aagcagcgag tcgccattac cggcatcggc gcgattaccc cgatcgggac cggggtcgag
     5101 gagctctggg ccggggtccg ttgcggccgg cccgcgatcc gcacgatcga tcggttcgat
     5161 cccgcccaga ccgcgagcaa gatggccggc cagatcgagt tcgatccgct gcgatacatg
     5221 acgccgaagc gcgcccgccg gcttgaccgc tattcgcagt tcgcgctggt ctcggcccag
     5281 atggcgctcg acgacgccgg tctctcggcc gaggaggctg ggtgcgccgg ggcggggatc
     5341 tacgtcggct cggcgttggg aggggtcgcc ttcgctgagg agcagcacga gggatatgtc
     5401 cgcaacgggc cgcacggggt caatcccttg cttgctctct cggtcttcgg cggcgcggcc
     5461 tcgtcgaacg tgacgatcga gcttggcctg accggtccgt ccctcgccta cggcaactcc
     5521 tgcgcggccg gcctgatcgc gatcggcgag gcggcacgac tcattgagga cggtcgggtg
     5581 ccggtcatgc tggccggtgg ggccgaggcg ccactctctc cgctcgtggt cggcgcgttc
     5641 gcgatgatcc gcgtcctcag cacccgcaac gacgatccct cgaccgcgag ccggccattc
     5701 gacaagggtc gcgacggctt cgtgatcgcc gaggggtccg ccgtcctcgt cctcgaggag
     5761 ttcgaccacg ccaagacccg cggcgcgcgg atctacgccg agattcccgg ctatgccacg
     5821 accagcgatg cttaccacat gaccgcaccc cacccggacg gtacccacgc cgcccgcgcg
     5881 atgtgcgccg cgatgcggag cgccgggctc tccccgagtg cgatcgatta cgtcaacgcg
     5941 cacggctcgt cgaccgtcct caacgacccg accgagtgcc gcgcgatccg caccgcgctc
     6001 ggtcgctacg cggagcgagt cgcggtcagc gggacgaagg ggctgcacgg tcatgccctc
     6061 ggcgcgaccg gtgcgatcga ggcggcgatc agtgcgctcg ctttgcagcg cggccacctt
     6121 cccggcaccg ccaacctggt cgatcaggac ccggcctgcg acctcgacat catcccgccc
     6181 ggcggccgcg atcagcaaat cgggcacctg ctctgcaacg ccttcggctt cggcggcgtc
     6241 aacgccaccc tcgccatgtc ctgctcagga agaggctcga tcgaacactg attgagcctg
     6301 gcgcttgcgc aactagcagc agatctgcga acccggcggg gcgccaattg aggtgcagca
     6361 caggctgcaa cacagatctg gtcttgggaa ggtgcacggt tggcctgacg gagtacagac
     6421 tgccgtcgcg gtcgcggtcg gtgttggcgt atccgttggc gtgcttgttg gcgtattggt
     6481 cggagtgtca gttggcgtgt ttgtg
//
LOCUS       DATAOZ010000301         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3018019, whole genome shotgun sequence.
ACCESSION   DATAOZ010000301 DATAOZ010000000
VERSION     DATAOZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3018019"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1493)
                     /locus_tag="VHR64_07925"
     CDS             complement(213..1493)
                     /locus_tag="VHR64_07925"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3302793.1"
                     /translation="MATSATKTGCCVVGGGPAGLVLGLILARAGVRTTVLEKHGDFLR
                     DFRGDTVHASTLTLLDELGLGEQFAAIPPRWIEKARVQLDSGTYSFADLTRLPGRHKH
                     IGLVPQWDFLNVVADASRREPSYELIMSAEVTDLLWDGQRVSGVSYVLDGQTQRLEAD
                     LVIGCDGRSSVVAERAGLRSRKFGVPMDVLWFRLPRLTTDPAGLVGRLSRSSLMGAID
                     RGEYLQLAVVIKKGSIDALRERGIELFRQRLHDGLPWLGDRIELLESFDDIRLLNVML
                     SRLRRWYRDGLLCIGDAAHAMSPVGGVGINLAVQDAVAAGRILAEPLLAGRVSTGSLR
                     KVQRRRWLPTVLTQAIQRLAHRVVIQASLAEDEVEPRSTTAPALLRLMQRFPVLQAIP
                     GYFVGIGLLPEHAPDFAKRASLGSAAHFSAKSPK"
     gene            complement(1567..2259)
                     /locus_tag="VHR64_07930"
     CDS             complement(1567..2259)
                     /locus_tag="VHR64_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302794.1"
                     /translation="MIWPKLFRRIVPYLAAGNTTFDGFDAWLRADPVHRDDRLLFANP
                     IESGNMHDVVAAAGNQIQLTRWPEDFVIEPDPPTPGTTTGRRRYTAGDVVIVAAVPDP
                     VGADLDHESASLLNVTPAEIDLQGWNLTDRARHPVSGFGVLAVLPGMEAKTAPPRFGH
                     AQIVLPAQRICQAGRIEAQYREDRQPWPPPRVELRQQLLDAVYAGRQFRLGRQRWTQW
                     IFWRRARSAAGD"
BASE COUNT          462 a          773 c          717 g          415 t
ORIGIN      
        1 cgaccttgtc gaaggttggg ctgtggtcca ggtggaggcc ggcgatgacg tcgcggctgc
       61 ggactaccga gtgatcaatg cgaccatgtt ctggtttccg gcgtaggcgg actccacggg
      121 gtgcacgccg tagatgaccg gtgtcttcaa ctcctcagcg ggccagtggc tccatcgccg
      181 cgaatgccgc ggaaaacggc ctgtgcacgg cctcatttgg ggctttttgc ggagaaatga
      241 gcagccgagc ccaacgaggc tcgctttgcg aagtcgggag catgttcggg tagcaggccg
      301 atccccacga agtagccagg aatcgcctgt aaaactggaa aacgctgcat aagcctgagc
      361 agggcggggg cagtcgtcga cctcggctcg acttcgtcct ctgcaaggct ggcctgtata
      421 acgacgcggt gcgcgagtcg ctgaattgcc tgcgtgagga ctgtcggtaa ccagcgtcgt
      481 cgctgaacct tgcggagact tccagtcgat acccgcccag cgagcagcgg ttcggcaaga
      541 atccggccgg ccgccacggc gtcctggaca gcgaggttga ttccgacccc tccgacgggt
      601 gacatcgcat gagcagcgtc gccgatgcaa agcaggccat ccctatacca gcgtcggagc
      661 cggctgagca tcacgttcag caaccttatg tcgtcaaatg attccagcag ctcgattcga
      721 tctcccaacc acggtaggcc gtcgtgcagc cgctggcgga acagctcgat accgcgctcg
      781 cggagcgcgt caatcgagcc cttcttgatc acgacggcga gctggaggta ctcccctcga
      841 tcaatcgcgc ccatcaaact tgaccgtgag agccgaccaa cgaggcccgc gggatcggtt
      901 gtcagccgcg gcagcctgaa ccacagcaca tccatcggaa caccaaactt ccgcgaccgc
      961 agtccggccc gctcagctac aacagaagat cggccgtcgc atccgatgac aagatcagct
     1021 tccagtcgtt gcgtctggcc gtccaggaca tagctcaccc cactgaccct ctgtccgtcc
     1081 caaagcagat cggtgacctc agcgctcatg atcagctcat agctcggctc gcgacgactc
     1141 gcatcggcga ccacattaag gaaatcccac tgcgggacga gaccgatgtg tttatgacgg
     1201 ccgggcaggc gcgttagatc ggcgaacgaa tacgtgccgg aatcgagttg gacgcgggct
     1261 ttttcaatcc agcgaggggg aatagcggcg aattgctccc ccagccccag ctcgtccagc
     1321 aaagtcagcg tagaggcatg cacggtgtcg cctcgaaagt cgcgcagaaa atcgccgtgc
     1381 ttctcgagca ccgtcgtgcg cacccccgcc cgggccagaa tgaggccgag cactaaccct
     1441 gccggtccac cgccgacaac acagcagccc gtcttagtcg cggacgtcgc catgtgacga
     1501 attatccgcg cgtcgatcta gaccagcgtt ggcggcagcg cggcttcgtt ctccagcagc
     1561 cagcgcttag tctccagctg ccgaccgggc tcgccgccag aatatccact gagtccatcg
     1621 ctggcgacca agccggaatt gccgaccggc gtagaccgcg tcgaggagtt gttgccgtag
     1681 ctctacgcgt ggcggcggcc aaggctgtct gtcttctcga tactgggcct caattcgtcc
     1741 cgcctgacag atcctctgag ctgggagcac aatttgcgcg tggccaaacc ttggaggtgc
     1801 cgtcttcgcc tccatgcctg ggagcaccgc gaggaccccg aaaccggaga ccgggtgcct
     1861 ggcgcgatcg gtaagattcc agccctgcag atcaatctcg gccggcgtta cgttcaacaa
     1921 cgacgccgac tcgtgatcga gatcggcacc caccggatcg ggaacggcgg cgacgatcac
     1981 cacatcgccc gctgtatagc gccgacgtcc cgttgtggtg ccgggtgtgg gcgggtccgg
     2041 ctcgatgacg aagtcttccg gccaacgcgt cagctgaatc tggttcccgg cggccgccac
     2101 aacatcgtgc atgttcccag actcgatcgg attagcgaac agcagacggt cgtcacggtg
     2161 aacgggatcg gcccgcaacc aggcgtcgaa accgtcgaac gtggtgtttc cagcggccag
     2221 atacggcacg atccggcgga acagtttcgg ccagatcacc agctcttcca gcgcggcaag
     2281 gtcgacaatc gtggccttac cgtttgggtc cgccgtcgag cgcgcccgga tcccagcctt
     2341 ctctgcccgc gcccgccgcg acgaatc
//
LOCUS       DATAOZ010000302         3974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7754366, whole genome shotgun sequence.
ACCESSION   DATAOZ010000302 DATAOZ010000000
VERSION     DATAOZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7754366"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1082)
                     /locus_tag="VHR64_07935"
     CDS             complement(162..1082)
                     /locus_tag="VHR64_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302795.1"
                     /translation="MPAIAQRFGTTLTRRQVTAGIAVAGAGLALARSVSFAQDASSTP
                     ATTSIPSGAAAWTRFNLNSANNDQFATIPGVGDRMLDEFNEYKPYSSILQFRKEIGKY
                     VDDSVVAAYEAYLFVPVDPNTADSDTLQQLPGVSSDIADQLLSAVPYADDAAFVAALG
                     SLVSADFAALASQYLSSTPSATATWLKFNLNTSSDDRFLMIPGVGDKMLREFNEYKPY
                     TSIEQFRQEIGKYVDDSQVAAWERYVYVPVDPASADAATLQQLPGVSEDDAKSLAESV
                     PYADAAAFSTALASVVSPEQAALAGSYIAQ"
     gene            1278..2267
                     /locus_tag="VHR64_07940"
     CDS             1278..2267
                     /locus_tag="VHR64_07940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX3302796.1"
                     /translation="MPFRVAVTRKLPELGEQMLTEAERRGEIQVVRWPNELPPFPQEL
                     AKLIHGADGVISLLTEPMTGEVFEREPQLRVVSNLAVGFDNIDVPAATRHKVAVCNTP
                     GVLTDATADMAFTLLMMTARRAKEAVDYVRDGKWETWSPTLLLGQEITGATLGVIGFG
                     RIGKEMARRGTGFGMRLLAHDEYQDDATAAQIGVTFTSLEELLRESDFVSLHCTLTPE
                     TRHLIGREQLAMMKPSAILINAARGPVVDTDALVDALRDGTIWAAGLDVTDPEPLPAD
                     HPLVSMPNCIVVPHIASATIATRNEMARLAVENCLAVLNGTTPPHCVNPEVLA"
     gene            2264..2473
                     /locus_tag="VHR64_07945"
     CDS             2264..2473
                     /locus_tag="VHR64_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302797.1"
                     /translation="MTGQESATAWPKGVSQPAIRALTAAGYSRLEELAGASESALLKL
                     HGMGPKAIRVIKESLAERGLAPMAL"
     gene            complement(2900..3793)
                     /locus_tag="VHR64_07950"
     CDS             complement(2900..3793)
                     /locus_tag="VHR64_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX3302798.1"
                     /translation="MSALLNVVLPVALIVAVGVIAGRRLPIDVASLTKLTLYVFAPAL
                     AADAMYRAQLGAEDAIRIFVAFSLTTFALYLFSRLVARISGLDLSARKSLIATTIFPN
                     SGNFGLSLTLLALGQEGLERGFVTFAASALFVFGLGPGLVSGQGVRQGIVTTARLPMI
                     WALIVGIGLRAGDITLPKGIADGLHLLAGATVPTLLITLGLQISRQKLAVLPSDVTAT
                     AMRLIGGPAAAYLAGRAVGLDHLALQVLVLQCATPTAVNALLITAEFGGDARKAARVV
                     VLSSLVCFATIPIVMALMGID"
BASE COUNT          745 a         1192 c         1352 g          685 t
ORIGIN      
        1 cacgcccagg aagccgattg cgcagagtgc gaacggcagc agttgcatcg tgccgtcgta
       61 gtgcttgagc atgatcagct cgccgctcgt gcccacgaag aggaacgcgg agatgccgag
      121 cagccagcgc cggatcgcgg cgaggcgatg atccgcgccc atcattgcgc gatgtaggag
      181 cccgccaacg ccgcctgctc cggcgagacg acggacgcga gcgcagtgga gaaggccgcg
      241 gcatcggcgt acggcacgct ttcggccagg gacttggcgt cgtcctcgga gacacctggc
      301 aattgctgga gcgtggccgc gtcggcgctg gctggatcaa ccgggacata gacgtaccgc
      361 tcccaggcag cgacctggga atcatcgacg tacttgccga tctcctgccg gaactgctcg
      421 atcgaggtgt acggcttgta ctcgttgaat tcccgaagca tcttgtcgcc gacgcccggg
      481 atcatcagga accggtcgtc cgaggaagtg ttgaggttga acttgagcca ggtcgcggtc
      541 gcagaggggg tcgaggacag atactgcgag gcaagtgccg cgaaatccgc cgagacaaga
      601 cttccgagcg ccgcgacgaa ggcagcatcg tcggcatagg gaacggctga gagcagttga
      661 tcggcaatgt cagacgagac gcccggcagc tgctggaggg tgtcggagtc ggccgtattc
      721 gggtcaaccg gcacgaagag gtaggcctca taggcggcga ccacggagtc gtcgacgtat
      781 ttgccgatct ccttgcggaa ctggaggatg ctcgagtagg gcttgtactc gttgaactcg
      841 tcgagcatac gatcgccgac gcctgggatg gtcgcgaact ggtcattgtt ggcactgttc
      901 aggttgaacc tggtccaggc ggccgcgccg cttgggatgg acgtcgtcgc aggcgtgctc
      961 gaggcgtcct gggcgaatga gaccgaccgg gccagcgcga gtccggcgcc cgcgacggcg
     1021 attccggccg tcacttgccg tctggtaagc gtggttccga agcgttgtgc aatggcaggc
     1081 acagcatgtt cctttcgtgt ccagatggac cgatatcgtc aattgggaaa tccggaattg
     1141 gaggtcccag ccaggtacga cggtacggaa ctgcgtatat ggtcagcgtt tgctcggtgc
     1201 gcctggtggt cacttgatgt gttccggtgc gatatgctga ccgggctggg aaatcactcg
     1261 atggggagga tccgaagatg ccgtttcgtg tcgccgtcac ccggaagctg cctgagcttg
     1321 gcgagcagat gctcaccgag gcggagcggc gcggcgagat ccaggtcgtc cgctggccga
     1381 acgagctgcc gccctttccg caagagctcg cgaagctgat tcacggggcc gacggcgtga
     1441 tttcgctcct gaccgagccg atgacgggcg aagtcttcga gcgcgagccg cagctccggg
     1501 tcgtcagcaa cctggcagtc ggcttcgaca acatcgatgt cccggcggcg acgcggcaca
     1561 aggtcgcggt ctgcaacacg ccaggcgttc tgaccgatgc caccgccgac atggccttca
     1621 cgctcctgat gatgactgcc cggcgcgcca aagaggcggt cgattatgtc cgcgacggaa
     1681 aatgggagac gtggtcgccg acgctgctgc tcggccagga gatcaccggc gcgacgctcg
     1741 gggtcatcgg cttcggccgg atcggcaagg agatggcgcg gcgcggcacc ggtttcggga
     1801 tgcggttgct ggcccatgat gaataccagg acgatgcgac cgccgcgcag atcggtgtca
     1861 ccttcacgag cctcgaggag ctgctgcggg agtcggattt cgtctcattg cactgcacgt
     1921 tgacccccga gacgcggcat ttgatcggcc gggagcagct ggcgatgatg aagccgtcgg
     1981 ccatcctgat caacgccgcg cggggaccgg tcgtcgacac cgatgcgctt gtcgatgcgc
     2041 tccgggacgg gacgatctgg gcggccgggt tggacgtgac cgatcccgag ccgctgccag
     2101 ctgatcatcc gctggtttcg atgccgaact gcatcgtcgt gccgcacatc gcttcggcga
     2161 cgatcgcgac gcggaacgag atggcgcggt tggcggtcga gaactgcctg gcggtgctga
     2221 acgggaccac gccaccgcac tgcgtcaacc ccgaagtcct ggcgtgaccg ggcaggaatc
     2281 ggcgacggcc tggccgaagg gcgtctcaca gccggcaatc cgggcactga ccgccgctgg
     2341 ctattcccgg ctggaagagc tcgccggcgc aagcgagtcg gcgctgctca agctgcacgg
     2401 catggggccg aaagcgatcc gcgtcatcaa ggaatcgctg gcagagcggg gtcttgcgcc
     2461 catggcactg tgaatcgaag acctgttctc tccatagatg ctcattttgg agtgaccgcg
     2521 ttgcaaaacg atcatctatg gcatctatgg catctatgga cctgggcagt tccattctgc
     2581 ctagcattgc gcagatatta tttccggtag gcggtttatg ttcaccatag atgtcatagg
     2641 cgcctaaggc gcgcgttttt ccacgcgtcc ttcgcgtcta atcatctatg gaggctacag
     2701 cgatttgcgc aatagttgac cgcctccttt acgtagcgcg gggttaccac gccccgcccg
     2761 gacccgatgc cgggatgtcc ataggagaaa accaaccccg ttcatgatcc ggctccatca
     2821 cccgtacgtg gggatgcgca agcggggcgt agtaaccccg cgctacgttt tcggcacatc
     2881 ggccccattc aacgcacgtt cagtcgatgc ccatcagggc catcacgatc gggatggtgg
     2941 cgaagcagac gaggctggag aggacgacga cccgggcagc cttgcgcgcg tcgccgccga
     3001 actcggccgt gatcagcagg gcgttgacgg cggtcggggt cgcgcattgg aggacgagca
     3061 cctgcagcgc gaggtgatcg agtccgactg ccctgccggc gaggtaggcc gctgccgggc
     3121 cgccgatcaa ccgcatcgcc gttgccgtga catctgatgg cagcacggcc aatttctggc
     3181 ggctgatctg gagaccgagc gtgatcagca gcgtcggcac ggtcgcgccg gccagcaggt
     3241 gcagtccgtc tgcgatcccc ttcggcaggg tgatgtctcc ggcacgaaga ccgatcccga
     3301 cgatcagcgc ccagatcatc ggcagccggg cggtcgtgac gatcccttgc cggacaccct
     3361 gcccgctgac caatccgggc ccgagcccga agacgaagag cgcgctggcg gcgaaggtca
     3421 cgaatccgcg ctccagcccc tcctgcccca gtgcgagcag ggtcagcgag aggccgaagt
     3481 tgccactatt cgggaagatc gtggtcgcga tcaggctctt gcgggcggag agatcgagtc
     3541 ccgagatccg cgcgaccagg cgggagaaga gatagagcgc aaaagtcgtc agcgaaaagg
     3601 cgacaaagat gcggatggcg tcctcggcgc cgagctgggc ccggtacatg gcatcggcgg
     3661 cgagggccgg cgcgaagacg tagagggtga gcttggtcag ggaagcgaca tcgatcggca
     3721 agcggcgccc ggcaatcact ccgactgcga cgatcagggc aaccggcagg acgacattga
     3781 ggagggcgct cacgcgggaa tagtacgggc tgtcatcttt ccccgttcgg cgtagcgaat
     3841 ttacgaccgg cccggtgcaa ggcagtccta cagtggtccc ggcatggact ccgctgtgat
     3901 gcggacatgc gaaaggagcc tgacatgccg tatgccaatc gaagcgaact tccggattcg
     3961 gtcaagaacc atct
//
LOCUS       DATAOZ010000303         3159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8282662, whole genome shotgun sequence.
ACCESSION   DATAOZ010000303 DATAOZ010000000
VERSION     DATAOZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8282662"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VHR64_07955"
     CDS             complement(<1..1071)
                     /locus_tag="VHR64_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3302799.1"
                     /translation="MPVVPDPANIEIKLPEPPERIGAIDPETQASRAAREAARVLEAE
                     LTARISGEVRFDRTSRMLYSTDASNYQIEPIGVVVPKTADDAIGAVELAASHGVPILP
                     RGGGSSLAGQTVGAALIIDFSKYCNRILDLNPEAGTVTVETGSSLDLLNRQLKANGLM
                     YGPDPSSSNRATIGGVIGNNSTGAHSILYGMTGDNVISTRITLSGGGTFELGAGTSDE
                     LTALAAHGDTRGRLLTQLLDYQERHSDLIARDFPPHWRRSTGYSLNEFIKPDFNPGRL
                     VVSSEGTLATVLSATMKVVPVPRKTALVVLQFADLVASMEATPTILETGPSAIELMDK
                     MLIDLTRAQPAYAPQIAFIEGDP"
     gene            1247..2080
                     /locus_tag="VHR64_07960"
     CDS             1247..2080
                     /locus_tag="VHR64_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302800.1"
                     /translation="MTVWMCATCGLEHADTPEPPDMCAICSDDRQYVPRTGQRWYTLP
                     QVQEGRSAEIVEMEADLYGITVTPKVGIGHRPLLVRTPTGNVLWDAPGFVDDALIGEI
                     AQLGGLAAIASSHPHLTGLSITISHAFGGVPVWYGANDQRWIRRPDDMIRLWRHRQEV
                     LPGVTLVQCGGHFPGSAVLHWAAAADGGGAILTGDTIRVNSDWHTISFMRSFPNLIPL
                     PPRSVQKIVDAVAPLAFDRIYSGFADELIETGGKEAVRFSAQRYIDWVTDKARDPDER
                     I"
     gene            complement(2277..>3159)
                     /locus_tag="VHR64_07965"
     CDS             complement(2277..>3159)
                     /locus_tag="VHR64_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3302801.1"
                     /translation="VPFAIGTETWGSIHCPSSFNGITGLRPTAGRVSRGGAMALSWTM
                     DKIGPMARSAADCALVLREIAGVMPADGTTLGQPETSADDRRNGFRIGLLKGSIAASA
                     PGVAANTAAAVEALREIGTVEEIELPDLPWDDAASIIIMCEAASAFESFLERGDGLKL
                     TAPEDRTGLYHALVVPAIDYLKAMRLRSHGAAEMAKLLAGYDALIAPAYPVTPPPAEG
                     NFEAYFDKHPGVSLSAMGNLLGLPSIAVPTGLDSDGLPSSLEILTTWWAEPTAVAIAS
                     AYQSKTTWHTAKPPLFA"
BASE COUNT          542 a         1006 c         1080 g          531 t
ORIGIN      
        1 cggatcaccc tcgatgaagg cgatctgcgg cgcgtaggcc ggctgggcac gggtcaggtc
       61 gatcagcatc ttgtccatca gctcaattgc cgagggaccg gtctcgagga tggtcggggt
      121 cgcctccatc gacgcgacaa ggtctgcgaa ctggagaacg accagcgcgg tcttgcgcgg
      181 gaccggaacg accttcatcg tcgccgaaag aaccgtcgcg agcgtgcctt cggacgaaac
      241 gaccagccgg ccggggttga aatcgggctt gatgaactcg ttgagcgagt agccggtcga
      301 gcggcgccag tgcggcggga aatcgcgcgc aatcaggtcc gagtgccgct cctggtaatc
      361 gaggagctgg gtcaggagcc ggccgcgggt atcgccatgt gccgccaggg cggtcaattc
      421 gtcggaagtg cccgccccaa gctcgaaggt gccgccgccg gagagcgtga tccgggtgct
      481 gatgacgtta tcgcccgtca tcccgtacag gattgagtga gcgccggtcg agttgttacc
      541 gatcacgccg ccgatcgtgg cccggttact ggacgacgga tccggcccgt acatcaggcc
      601 gttcgccttg agctggcggt tgagcagatc gagcgaggag ccggtctcga ccgtcactgt
      661 gcctgcttcg ggattcaggt cgaggatgcg attgcagtat ttcgagaagt cgatgatcag
      721 cgccgcacca accgtttgcc cagcgaggga ggacccaccg ccgcgcggca ggatcggcac
      781 cccgtggctg gcggccagct cgaccgcgcc gatcgcgtcg tcggccgtct tcgggacgac
      841 gacaccgatc ggctcaatct ggtagttcga ggcatcggtc gagtagagca tccgcgacgt
      901 gcggtcgaag cggacctcgc cgctaatacg ggcggtcaac tcggcctcga ggacccgcgc
      961 agcctcgcgc gcggcgcgac tggcctgcgt ttcggggtcg atcgctccga tccgctcggg
     1021 tggttccggc aacttgatct cgatgttagc ggggtctggt acaaccggca tggagcgccg
     1081 tcttcctcta cgctgcgccg aaacgcgctc ggtggactca cgtgaaggaa ttgtaggcgc
     1141 ggagtcgcaa aggcgcggag gcgcggagtt tggtagaggg aacgaaaacc caaaacctga
     1201 aacctggaac agtttgtatg tccacggaac aagagaggtg ccatcgatga ccgtttggat
     1261 gtgcgcgacg tgcgggctgg agcatgcgga tacaccggag ccgccggata tgtgcgcgat
     1321 ttgctctgat gatcggcagt acgtgccgcg gaccggccag cgctggtaca cgctgccgca
     1381 ggtgcaggag ggccggtccg cggagattgt cgagatggag gccgatctct acggcatcac
     1441 ggtcacgccg aaagtcggga tcgggcatcg cccactgctt gtccgcacgc cgaccgggaa
     1501 tgtgctctgg gatgcgccgg gtttcgtcga cgacgccctg attggagaga tcgcccagct
     1561 cggcggtttg gccgcgatcg cgtccagtca tccacacctt accggtctct cgatcacgat
     1621 cagccacgcg ttcggcggag tgcccgtctg gtatggcgcc aacgaccagc gctggatccg
     1681 ccgcccggac gacatgatcc ggctctggcg gcatcggcaa gaggtcctgc ccggcgtgac
     1741 cctcgtccaa tgcggcggac acttccccgg tagcgcggtc ctgcattggg cggcagccgc
     1801 cgatggggga ggcgcgatcc tgaccggcga cacgatccgg gtcaactccg actggcacac
     1861 catctccttc atgcgcagct tcccgaacct gatcccactg ccgccgcgct cggtccagaa
     1921 gatcgtcgat gcggtcgcgc cgctggcctt cgaccggatc tacagcggct tcgcggatga
     1981 gctgatcgag accggcggga aagaggccgt gcgcttctcc gcccagcgct acatcgactg
     2041 ggtgacggac aaggcacgcg atccggacga acgaatctga ggcgagcagg cgagcagacg
     2101 gatgatagtt tctccctctc ccgaaatcgg gagagggcgg gtgagggtcg ctgccggaat
     2161 gatcgtttct gaggtacaac cggttgatcc ccgttaccat cccggctctg gtgacgctac
     2221 gcgcggattc gcagccctca tcccctgccc ttctcccgtg aacgggagaa gggagatcag
     2281 gcaaacaacg gcggcttggc ggtgtgccag gtggttttgg actggtaggc ggaggcgatc
     2341 gccacggcgg ttggctccgc ccaccaggtc gtcaggatct cgaggctgct gggcaatccg
     2401 tcgctgtcga ggccggtcgg gactgcgatt gatggcagcc cgagcaggtt gcccatcgcg
     2461 ctcaggctga cccccgggtg cttgtcgaag taggcctcga agttgccctc ggccggcggc
     2521 ggcgtgactg gataggccgg cgcgatcaag gcgtcgtacc cggccagcaa cttcgccatc
     2581 tcggccgcgc cgtggctgcg caaccgcatc gccttcaggt agtcgatcgc cgggacgacc
     2641 agcgcgtgat agagcccggt acggtcctcg ggcgcggtca gcttcaagcc atcaccccgc
     2701 tcgaggaacg attcgaaggc cgaggccgcc tcgcacatga tgatgatcga cgcggcgtca
     2761 tcccacggca gatcgggcag ctcgatctcc tcgaccgtgc cgatctcgcg gagcgcttcg
     2821 accgccgccg ccgtattggc cgcaacaccc ggagcacttg cggcgatcga gcccttgagc
     2881 aacccgattc ggaagccgtt ccggcgatca tcggccgacg tctccggttg gccgagcgtc
     2941 gtgccgtcgg ccggcatcac gccagcgatc tcccgcagca ccagcgcgca gtcggcggcc
     3001 gagcgggcca tggggccgat cttgtccatg gtccacgaga gcgccatcgc cccgccacgg
     3061 ctgacccggc cggcggtggg gcgcagaccg gtgataccgt tgaaggacga cggacaatgg
     3121 atcgagcccc acgtctcggt gccgatcgcg aacgggacc
//
LOCUS       DATAOZ010000304         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_650324, whole genome shotgun sequence.
ACCESSION   DATAOZ010000304 DATAOZ010000000
VERSION     DATAOZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650324"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VHR64_07970"
     CDS             <1..334
                     /locus_tag="VHR64_07970"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3302802.1"
                     /translation="PAREHQGYEGIVHGGLISTVLDEVMGWAIYVRKLWAVTGTMNVR
                     FRKPVRVGDALTARAWVESVTGRKVDVRAQLVRNSDGQVLADATAIFIRVSEAQASEW
                     QGRYFDQP"
     gene            371..835
                     /locus_tag="VHR64_07975"
     CDS             371..835
                     /locus_tag="VHR64_07975"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3302803.1"
                     /translation="MLEHDRWATSTMLDVSRDLSDEQLDRSYDIGLGSLRATFGHIIV
                     NVDFWIRLMTGDPIEYEELTRASIADLIQHHDRTYAAFAALARRLRDEQCLDDIFLDH
                     YEVNKSFGGTILHVVLHNAEHRTEAVHILQRLGLPNVPEVDHGLWDYEMHNT"
     gene            complement(843..1472)
                     /locus_tag="VHR64_07980"
     CDS             complement(843..1472)
                     /locus_tag="VHR64_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302804.1"
                     /translation="MSDAWSSRIAAFVVQALDLEIEPQDLIPEIVALKRDDASGISAV
                     ELQSSVGPTPFLVYHYQVGAGDQSQFNADIATLEKAATLDTPGPRIVAHATSGDEAFI
                     LATTPSVQRSLTGRAEALPTRPPMPVERARTRVPDRLIDKLREANRLAGDWLIAIQTA
                     GESGDDLSLTDQEAALALFLLDDRSIQDLLRALNVLISTARNQAGEALG"
     gene            1505..>1822
                     /locus_tag="VHR64_07985"
     CDS             1505..>1822
                     /locus_tag="VHR64_07985"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX3302805.1"
                     /translation="MDLEQAQMLMDYARAATASAYVPYSKFPVGAAVMTESGAIFTGV
                     NIENCSSGATVCAERVAIFKAVSEGYRRLRAIAIYAPNAPKRGSSCGCGICRQVMAEF
                     GLDA"
BASE COUNT          350 a          572 c          560 g          340 t
ORIGIN      
        1 tcccgcgcgg gagcaccagg ggtatgaagg gatcgttcac ggcggtctga tttcaaccgt
       61 gctcgacgag gtgatgggat gggcgatcta cgtccgcaag ctctgggcgg tcaccggcac
      121 catgaatgtc cgtttccgca aaccggtccg cgtcggcgac gcgctcaccg cccgggcatg
      181 ggtcgaatcg gtcactggtc gcaaggtcga tgtccgcgcc caactagtcc gtaacagcga
      241 cggtcaagtg ctggccgatg caaccgccat cttcattcgc gtctccgaag cgcaagccag
      301 cgaatggcag ggccgctact tcgaccagcc atagcgcaca ttgggggtat gaatggactt
      361 gttggatcgt atgctggaac acgatcgctg ggccacatcg acgatgctcg acgtatcacg
      421 tgacctcagc gatgaacaac tcgacaggtc gtacgacatc gggcttgggt ctctccgtgc
      481 cacgttcggc cacatcatcg tcaacgtcga cttctggatc aggctcatga ccggggatcc
      541 gatcgaatac gaagaactga cgcgtgcttc gatcgcggat ctcattcagc accatgaccg
      601 aacctacgcg gccttcgcag cgctcgcccg ccgcttgcgc gacgagcagt gcctggacga
      661 catctttctg gaccactatg aagtgaacaa gtcgttcggg ggcacgattc tgcacgtcgt
      721 cctgcataac gccgagcacc gcaccgaagc ggtccacatt ctccagcgtc tcggactgcc
      781 gaacgttccc gaagtcgatc atgggctctg ggactacgaa atgcacaaca cgtaagcgct
      841 tttcagccga gagcctcgcc ggcctgattg cgcgcagttg agatcaggac gttgagagcc
      901 cgcaacagat cctggatcga gcgatcgtcg agcagaaaga gagcgagcgc ggcctcctga
      961 tcggtgaggc tcagatcatc tccgctctct ccggcggttt ggatcgcgat cagccaatcc
     1021 ccggcgagcc gattcgcctc acgtagcttg tcgatcaacc ggtccgggac ccgcgtcctc
     1081 gcccgctcga ccggcatcgg cggccgcgtc ggcaaagctt cggcccgacc ggtcaacgag
     1141 cgctgaactg acggtgtggt cgcgaggatg aacgcctcgt cgccgctggt cgcgtgcgcc
     1201 acgatgcgcg gtccgggcgt atcaagggta gcggcctttt cgagcgtcgc gatgtccgcg
     1261 ttgaactgcg attggtcccc agccccgacc tggtagtggt agaccaggaa tggagtgggc
     1321 ccaacgctcg actgcagctc cacggcactg atgccggagg cgtcgtcgcg tttcagtgcg
     1381 acaatctccg gaatcaaatc ctgcggctcg atctcgagat cgagcgcctg gacaacgaat
     1441 gcggcgattc ttgacgacca tgcatctgac atatcgcgat gataccggga aaggctgacg
     1501 actgatggac ctcgagcagg cgcagatgct catggactac gcccgcgctg cgaccgcttc
     1561 ggcctatgtg ccctactcca aatttccggt cggcgcggcg gttatgaccg agagcggcgc
     1621 gatcttcacc ggcgtcaaca tcgagaactg ctcgtccggc gccacggtct gtgccgaacg
     1681 ggtcgcgatc ttcaaagccg tttcggaagg ctaccgccgg ctgcgagcga tcgccatcta
     1741 cgcacccaac gctcccaagc gcggatcgag ctgtggctgc ggcatttgcc ggcaggtgat
     1801 ggcggaattc ggattggacg cg
//
LOCUS       DATAOZ010000305         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3282313, whole genome shotgun sequence.
ACCESSION   DATAOZ010000305 DATAOZ010000000
VERSION     DATAOZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3282313"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..187)
                     /locus_tag="VHR64_07990"
     CDS             complement(20..187)
                     /locus_tag="VHR64_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302806.1"
                     /translation="MMVQTVDPVVGTSGAVIPAVTIPGLKPRADRESLSETGRVFRGI
                     TDPDIDGFLGA"
     gene            645..3248
                     /locus_tag="VHR64_07995"
     CDS             645..3248
                     /locus_tag="VHR64_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3302807.1"
                     /translation="MATTSETPTKRRKTTAAADADTAQEQSITEAWNAGDDFASAPAI
                     EEEAAAPKRTRKKKTDEPVAETAPTTEEDAVEAVAVEAKPKRTRKKATEETPEAVEVT
                     AEEPVEAAAEPVAEAAPVVEEAEKPKRSRKKAETDEAPKSLIEIASEKPSTQAADAAP
                     QVAQVEEIQEETPVTFADLGMSDSLMKSITAVGYEEPTPIQLSTIPLLLEGHDVIAQA
                     QTGSGKTAAFGLPIIEAVNPKQRNVQALILAPTRELAIQVSEALHKYGRHKEVETLPI
                     YGGQPYERQFRGLQRGPQIVVGTPGRVMDHMRRQTLDLSHIKFFILDEADEMLDMGFI
                     EDIEWILEQVPTERQTALFSATMPPRIVQLASRYLNNPERISVASKQMTVPEVTQAFY
                     EVPRNRKIDALTRILDAEVPSLAMIFCRTKMMVDELGEALMARGYSAETLHGDLSQAQ
                     RDRVMRRFRTGQADILIATDVAARGLDIPDVSHVINYDIPESPETYVHRIGRTGRAGK
                     TGVAITLVTAREVRWIRQIERAVKARIEPRRLPTVADVAERRVEQLKNQITSALGDEV
                     APALAWYVSAVEAMAEEYELSTIAAAALRLYAEETGRGQIEEQVDDVAVIAIQSGAPR
                     DRGDRFDRGQGRDDRRGPRSFEPSEGKVRLFMNVGHNLGIRPQDIVGAIANEANIPGR
                     TIGSIDIFDGYSFVEVPSDDSQRIIDALSQSGIKGKYVNVEVARPDAGPGQRRDRFDR
                     GDRGDRFDRGGGGYRGDRGGERRFDRPEPGNWRGRDNDRDRGPRPPFDRDRAPARRDF
                     EDRAPRFDRDDRAPRFDRDERAPRFDQDRAPRFERDNDRGNRFDRPDRGNRFERSDRP
                     RNPGRPDRDRF"
     gene            3257..3769
                     /locus_tag="VHR64_08000"
     CDS             3257..3769
                     /locus_tag="VHR64_08000"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX3302808.1"
                     /translation="MRRSTHLIMGAAAAAPLAALMPPVGAVGCLWLGVTGGAFPDYVD
                     LRSGMKRHLKHRGFSHSYLMLALSTAFVYVVLRALNQQEVSDTLRVSLDYVRAWSAAF
                     AFGVMSHLLGDACTRGGIQPALPFSQRRVWVLPKIFRGKSVGWQNGVAQVIAIMVLGV
                     SLATFLTLQR"
BASE COUNT          698 a         1337 c         1252 g          605 t
ORIGIN      
        1 aaaatcagct ccgcggtggt cacgcgccga gaaaaccgtc gatgtcagga tcggtgatcc
       61 cacggaacac gcgtccagtc tcggagaggc tttccctgtc agcccggggt ttcaaccccg
      121 ggatcgtcac cgccgggatc accgcaccgc tggtccccac aaccgggtcc accgtctgca
      181 ccatcatgcc gcttcgcgtc atcgcaggga tgccgtgccg cgctccgtga tgggaatacg
      241 gccccgtcag cctgctttag ctggcttatt ggattcagcc cggtgattta tcgccgggcg
      301 gtgtcgatcg aaacgccacc agcaccaaga ggcaaactgg caggtgagac aaggcgagta
      361 cggagcctcg ccactacatc taactccgcg cctccgcgcc tcctatcttc gcgcctccgc
      421 gcctgctata cttaccagcc gaacgccgca gtgtggccgt tctcgggatc cccaaagttc
      481 cttccgagtg tgtgtgctgg gtgttgtgag tagtgtgtgg ccgcgcccgc ggcccaatgg
      541 gcgccgagcg ctcttccgtg gtccaaccac gtctgtggtt attgtgggga atagggtttg
      601 gaccccgata ttgcgcaagg catcgtgtaa agaggatgcc atccatggca acaacgagcg
      661 agacgccgac caaacggcgt aagacaacgg ctgccgccga cgccgatacg gcgcaggagc
      721 agtcgattac cgaggcgtgg aatgccggcg acgatttcgc cagtgcgccc gcaattgaag
      781 aggaagcggc cgctcccaag cggacccgca agaagaagac cgatgagccg gttgccgaaa
      841 cggccccaac cacggaagag gatgccgtcg aggccgtcgc cgtagaggcc aagcccaagc
      901 gcacccggaa gaaggcaacg gaggagaccc ccgaggccgt cgaagtgacc gcggaggagc
      961 cggtcgaggc tgctgctgag ccggtggctg aagccgcacc ggtcgtcgag gaggcggaga
     1021 agccgaagcg ttcccgcaag aaggcggaga ctgacgaggc gcccaagagc ctgatcgaga
     1081 tcgcgagcga gaagccgtcg acacaggccg ccgacgccgc gccgcaggtc gcgcaggtgg
     1141 aagaaatcca ggaggagact ccggtcacct ttgccgatct cggcatgagc gattcgctca
     1201 tgaagagcat cacggccgtc ggctacgaag agccgactcc gatccagctc tccacgatcc
     1261 cgctgctgct cgagggtcac gatgtgatcg cccaggcgca gaccggctcc ggaaagacgg
     1321 ccgccttcgg tctgccgatc atcgaggcgg tcaacccgaa gcagcgcaat gtgcaggccc
     1381 tgatcctggc cccgacccgg gagctggcga tccaggtctc cgaggcgctt cacaagtacg
     1441 ggcgccacaa ggaagtcgag acgctcccga tctacggcgg tcagccctac gagcgccagt
     1501 tccgcggtct ccagcgcggt ccccagatcg tggtcggcac gcccggccgc gtcatggacc
     1561 acatgcgccg ccagacgctc gatctgtccc acatcaagtt cttcatcctc gatgaggcgg
     1621 acgagatgct ggacatgggc ttcatcgaag acatcgagtg gatcctcgaa caagtcccga
     1681 ctgagcgcca gaccgcgctc ttctcggcca ccatgccgcc ccggatcgtg cagctggcct
     1741 cccgctacct gaacaatccc gagcgaatct cagtcgccag caagcaaatg acggtcccgg
     1801 aagtcacgca ggccttctac gaagtcccgc gcaaccggaa gatcgacgcg ctgacccgaa
     1861 tcctggacgc ggaagtgcca tcgctggcga tgatcttctg ccgcaccaag atgatggtcg
     1921 acgagctcgg cgaggcgctg atggcccggg gctactcggc cgagacgctg cacggcgatc
     1981 tctcgcaagc ccagcgcgac cgcgttatgc gccgcttccg cactggccag gccgatatct
     2041 tgattgcgac cgacgtcgcg gcccggggtc tcgatatccc ggacgtcagc cacgtcatca
     2101 actacgacat cccggagagc cccgagacct acgtccaccg catcggccgc actggccgcg
     2161 ccggcaaaac cggcgtcgcg atcacgctcg tgactgcccg tgaggtccgc tggatccgcc
     2221 agatcgagcg tgccgtcaag gcccgtatcg agccgcgccg cctgcccacc gtggcggacg
     2281 tcgccgagcg ccgggtcgag cagctcaaga accagatcac gtctgcgctg ggcgatgagg
     2341 tcgcgccggc cctggcctgg tacgtctctg cagtcgaggc gatggctgag gagtacgagc
     2401 tgtccacgat cgccgccgcc gccctgcgcc tctatgcgga ggagacgggt cgcggtcaga
     2461 tcgaggagca ggtggacgat gtcgccgtca tcgccatcca gagcggtgcg ccgcgtgacc
     2521 gcggcgatcg gttcgaccgc ggtcaaggcc gcgacgaccg ccgtggcccg cgcagctttg
     2581 agccgtccga aggcaaggtc cggctgttca tgaacgtcgg ccacaacctc ggcatccgtc
     2641 cgcaggacat cgtcggcgca atcgcgaacg aggccaacat ccccggtcgc acgatcggct
     2701 cgatcgacat cttcgacggc tacagcttcg tcgaagtgcc gtccgatgac tcccagcgga
     2761 tcatcgacgc cctctcgcag agcggcatca agggcaagta cgtcaacgtc gaggttgctc
     2821 gaccggacgc cggccccggc cagcgccggg atcgcttcga tcgtggagat cgtggcgatc
     2881 ggttcgaccg cggcggcggt ggctaccgtg gcgatcgcgg cggcgagcgc cgcttcgatc
     2941 ggccggagcc gggcaactgg cgcggccggg acaacgatcg cgatcgtggc ccgcgcccgc
     3001 cgttcgaccg ggaccgcgcg ccagcccgcc gcgacttcga ggatcgggct ccgcgcttcg
     3061 accgcgatga tcgcgcgccc cggttcgacc gggacgagcg tgccccgcgc tttgatcagg
     3121 accgggcacc acgcttcgag cgggacaacg accggggcaa ccggttcgac cgcccggacc
     3181 gcggcaatcg gttcgagcgg agcgaccggc cccggaatcc gggtcgccca gaccgcgatc
     3241 gcttctagac caacgcatgc gacgatcgac ccacctgatc atgggtgcgg ccgcagcggc
     3301 gccactggcg gctctcatgc cgccggtcgg cgccgttggt tgtctctggc tgggcgtgac
     3361 cggcggcgcc tttccggact acgtcgatct gcgcagcggc atgaagcgcc acctcaagca
     3421 tcgcggcttc tcgcacagct acctgatgct tgccctcagc acggcgttcg tctatgtcgt
     3481 tctgcgcgcc ctcaaccagc aggaggtcag cgacacgctg cgggtgtcgc tcgactacgt
     3541 ccgggcctgg agcgccgcct tcgccttcgg agtaatgtcg catcttctgg gcgatgcctg
     3601 cacccgcggc ggcatccagc cggccctgcc cttctcgcag cgtcgcgtct gggtgctgcc
     3661 gaagatcttc cggggcaagt ccgtcggctg gcaaaacggc gtcgcccagg tcatcgccat
     3721 catggttctg ggcgtctccc tcgccacctt cctcaccctc cagcgctgac gagagagcgt
     3781 cccccgacta tggaggttgc ggccacgtcg ccccggactg caggccgggg ctgagtcaaa
     3841 ataaagcggc taaagccgac tggtggattg gcaaacgtag cgccggctcc ct
//
LOCUS       DATAOZ010000306         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1178266, whole genome shotgun sequence.
ACCESSION   DATAOZ010000306 DATAOZ010000000
VERSION     DATAOZ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178266"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..363
                     /locus_tag="VHR64_08005"
     CDS             112..363
                     /locus_tag="VHR64_08005"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3302809.1"
                     /translation="MIAAAGTRLEHDRVQAGVRKDIAGIAAATVDEYVQIDWDGKVLD
                     KTATLARIRSSNIQVQSNTLDEVSLRIYGEIAVVTGFRA"
     gene            complement(502..1227)
                     /locus_tag="VHR64_08010"
     CDS             complement(502..1227)
                     /locus_tag="VHR64_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX3302810.1"
                     /translation="MTTKQTWVAVIMAALCQLFVACGDHPTPIPTAPSPIPVVAAPVP
                     SPQPAPPAGTPIRPSIAAVQPSVGSTGGGAWGAITGRDFQPGATVLIGGQRAAVAAMQ
                     NNTTMLFWTATHDAGSVDITITNPGGLSDTLPGAYTYASPATFDPDAEWIGHAGPDFD
                     TEFHFSIRQGRLVDLTCVSTRLTPAAPTAVENGEFAYRSDTGASLSGRLVSPVNAVGT
                     IGLADCATGAWWAEKGPTQRVAK"
     gene            1310..>1852
                     /locus_tag="VHR64_08015"
     CDS             1310..>1852
                     /locus_tag="VHR64_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3302811.1"
                     /translation="MTPERWKQIEELFHEAEVLPATERIGFVRASPVDEGIRREVESL
                     LDEADADGDLLNFPLPRPAPDVRSDPVPIALTGKTIGNYQIQTLLGAGGMGEVYRARD
                     RRLGRDVAVKVLPAAFMNDPDRLARFAREARMLALLNHPGICAIYGIDEADGIRLLVL
                     ELVDGQTLAEKLATRAESGDA"
BASE COUNT          336 a          556 c          660 g          300 t
ORIGIN      
        1 cgaggtgcag ttggcgctgc attgacagaa cgaagtggta cgtccacaac atgaacaccg
       61 gcgcgatgca acaggggctc atgatgaaac gcgactactt ggctgttgat tgtgatcgcc
      121 gctgccggga cccggctcga acacgatcgt gtccaggccg gagttcgtaa agacatcgcg
      181 ggcatcgccg cggcgaccgt cgacgaatac gtgcagatcg actgggacgg caaggtgctg
      241 gacaagacgg cgacgctggc gcggatcagg tcgagcaaca ttcaggtcca gtcgaatacg
      301 cttgacgagg tcagcttgag gatctacgga gagatcgcgg tcgtcaccgg ctttcgcgcg
      361 tagaggcgtc atggatggca aggacatcag cgccgctatc cgctataccc gtatgtatgt
      421 cagaagagag ccgcggtggc aggttgtgcc gttcagcaga cgcggtcacg ccgagcgccc
      481 ggctgaggac gcaatcgctg ctcacttcgc gaccctctgc gtggggccct tctcggccca
      541 ccaggctccg gtcgcgcagt cggccaggcc gatggtgccg acggcattga cgggcgacac
      601 cagcctgccg gacaacgacg cgccggtgtc gcttcgataa gcaaactcgc cgttctcgac
      661 cgcggtcggc gccgcgggcg tgagacgggt tgacacacag gtgagatcga ccaggcgccc
      721 ctggcggatg ctgaaatgga attcggtgtc gaagtccggg ccggcgtggc cgatccactc
      781 ggcgtcggga tcgaaggtcg cgggtgacgc gtacgtgtat gcgccaggga gtgtgtcgct
      841 gaggccgccg ggattcgtaa tcgtgatatc gacggagccg gcatcgtggg tggcggtcca
      901 gaacagcatc gtggtgttgt tctgcatcgc cgccacggct gcccgctgac caccgatcag
      961 caccgtggcg ccgggctgaa aatcgcggcc cgtgatggcg ccccaggcgc cgccgccggt
     1021 cgagccgacg ctgggttgga ccgcggcgat cgatggccgg atcggcgtcc cggccggcgg
     1081 cgcaggctgt ggcgaaggaa ctggtgcagc gaccaccggg attggcgatg gcgccgtcgg
     1141 gatcggggtc ggatggtcgc cgcaggccac aaacaactgg cacagggctg ccatgatgac
     1201 ggccacccac gtctgtttcg ttgtcacagc tctacctcct acctgggaag cggaaaggaa
     1261 ggctggaaac atgaagccgg tgggagcgta gaatgctgac accgcgcaga tgacccccga
     1321 acggtggaaa cagatcgagg agctctttca cgaggccgag gtgttgccgg cgacggagcg
     1381 gatcgggttc gtgcgggcgt cgcccgtgga tgaaggtatt cggcgcgaag tggagtcgct
     1441 gctcgacgag gcggacgcgg acggggacct cctcaacttc cccttgccgc gccccgcacc
     1501 ggacgtccgg tcggatcccg tcccgatcgc gctgaccggc aagaccatcg gcaactacca
     1561 gatacaaacg ctgctcggtg ccggcggcat gggcgaggtg taccgggccc gtgaccggag
     1621 gctgggccga gatgttgcgg tgaaggtcct gcccgcggcc ttcatgaacg atcccgatcg
     1681 gctggcccgg ttcgcgcggg aggcgcggat gctcgcgctc ctgaatcacc caggcatttg
     1741 cgcgatctac ggcatcgacg aggcggacgg tatccgcttg ctggtgctcg aactggttga
     1801 cgggcagaca ctggcggaga aactggcgac acgtgccgaa tcgggtgacg cc
//
LOCUS       DATAOZ010000307         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3283294, whole genome shotgun sequence.
ACCESSION   DATAOZ010000307 DATAOZ010000000
VERSION     DATAOZ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3283294"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VHR64_08020"
     CDS             <1..588
                     /locus_tag="VHR64_08020"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3302812.1"
                     /translation="GDQVRYFLEEIAAMGPEIKGIRRIVQDEPDPEFCLQPRYIEGTR
                     ALPDYGLSADLCCNFRQLGPTVELVRQCPNTQFILDHIAKPNIRGGEMEPWASQMRDL
                     ASLPNVMCKISGVVTEANHDSWTIDEIRPYVMHALEVFGEDRVAFGSDWPVCTLATPY
                     RRWVETLDELTADWSDAAKSKLWRENAIRFYRLDA"
     gene            627..2072
                     /locus_tag="VHR64_08025"
     CDS             627..2072
                     /locus_tag="VHR64_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3302813.1"
                     /translation="MPIVEGMRWYHEHDFVSFATPGHRLGKGADPALMDLFGPQALEL
                     DMPVSGGATDVHFQHDVIREAEILGADAWGSDVCHYLVNGSSAGNLAYFLAHLAPGDR
                     VIIARDLHKSLMTALIQTGVQPIYIAPKLHPELDFGIGYDPDQVERAILANPDAKLVA
                     LVSPSYSGVASDLRAIAAVAHRHNVPVYVDEAWGPHFHFHPDLPESAMASGIDGAVAS
                     THKVLAAFTQSAVLNAQGPRVDYARLNTTVGMSQTTSPAAFIYASIDAARSQMALRGH
                     ELLDKALELAHEARERLRAIPGLTVVDGETLGVTRYDPTKFVLDVAGMGITGFEAEEE
                     LRTRFFINPEASDLVSVVCFVTIGDTRTSIDRLVTAFETLSNENRGRPRIDTHLRSSG
                     SVIRPGIQAMTPRDAFFARSRAVPLREAAGEISADLIIPYPPGIPVIAPGDVIEAHKL
                     DYLEHGVQAGFYISGAADQTLATIRIVSAHP"
     gene            complement(2069..3034)
                     /locus_tag="VHR64_08030"
                     /pseudo
     CDS             complement(2069..3034)
                     /locus_tag="VHR64_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(3047..3235)
                     /locus_tag="VHR64_08035"
     CDS             complement(3047..3235)
                     /locus_tag="VHR64_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302814.1"
                     /translation="MDRYAAEKVRSFGTSVFAEMSRLAVQHRAVNLGQGFPDFAGPDF
                     VKEAAKRAIDADLNQYAV"
     gene            complement(3242..>4216)
                     /locus_tag="VHR64_08040"
     CDS             complement(3242..>4216)
                     /locus_tag="VHR64_08040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX3302815.1"
                     /translation="GRYLELRRRLLGVDQLELYDTWVPLSTLPVVHYSWQEAVEIVCE
                     GLAALGDEYVSIMREGLTSGRWVDVHETKGKRSGAYSWGAYGSHPVILMNWNGTLSDV
                     FTLAHEAGHAMHTYLADRSQTYQDAQYPIFLAEIASTLNEVLLTWHLLSKIPAEKAME
                     RFAILNRFADQIMGTHVRQTMFAEFERETHRIVEAQQPLTLQQLNVLYGDLYRTYSPG
                     MNVDERGEISWSRVPHFYMSFYVYQYATGISAGISLAKQVRDEGAAAVERVMRLFESG
                     GKDYPLPLLKAAGVDLTTPDPIEACMAVFNETVSELEAIAASGAFDAA"
BASE COUNT          761 a         1397 c         1329 g          729 t
ORIGIN      
        1 ggcgatcagg tccgctactt cctcgaggag atcgcggcga tgggccccga gatcaagggc
       61 atccgccgga tcgtccagga tgagcccgat ccggagttct gccttcagcc gcgctacatc
      121 gaagggacgc gcgcgctgcc cgattacggc ctgtcagcag acctctgctg caacttccgc
      181 caactcggcc cgactgtcga gctggtccgg caatgcccga atacgcagtt catcctcgac
      241 catatcgcca agccaaacat ccgcggcggc gagatggagc cgtgggccag ccagatgcgc
      301 gacctcgcct cgctcccgaa cgtgatgtgc aagatcagcg gcgtcgtcac cgaggccaac
      361 cacgactcgt ggacgatcga cgagatccgg ccgtatgtca tgcacgcgct cgaagtcttc
      421 ggcgaggatc gcgtcgcctt cggcagcgac tggccggtct gcacgcttgc gacgccctat
      481 cgacgctggg tcgagacgct cgacgagttg acggccgact ggtccgacgc ggccaagtcc
      541 aagctctggc gcgagaacgc gatccgcttc tatcgcctcg acgcctgaca ccaaacatga
      601 tcaagagcgc cgttgaccat acccgcatgc caatcgttga agggatgcgc tggtaccacg
      661 agcacgactt cgtctcgttc gcgacgcccg gtcaccgcct cggcaaggga gcagaccctg
      721 ccctgatgga tctcttcggt ccgcaggccc tcgagctgga catgccggtc agcggcggcg
      781 cgaccgacgt ccatttccag cacgacgtca tccgcgaggc cgagatcctc ggcgcggacg
      841 cgtggggctc agacgtctgc cactacctgg tcaatggttc gtcggccggc aacctggcct
      901 acttcctcgc ccacctcgcg cccggcgaca gggtgatcat cgcccgtgac ctgcacaagt
      961 cgctgatgac cgcgctgatc cagaccggcg tgcagccgat ctacatcgcg ccgaagctgc
     1021 acccggaact cgacttcggc attggttacg accccgatca ggtcgagcgt gcgatattgg
     1081 ccaacccgga cgcaaagctg gtcgcacttg tcagcccgtc atacagcggc gtcgcatccg
     1141 acttgcgggc aatcgccgcg gtggcgcatc ggcataacgt gccggtctac gtcgacgagg
     1201 cgtggggacc gcacttccac ttccacccgg atctgccgga atcggcgatg gccagtggaa
     1261 tcgacggcgc ggtcgccagc acgcataagg tcctggccgc gtttacgcag tcggctgtcc
     1321 tcaacgcgca gggtccgcga gtcgattacg cgcgcctgaa cacgaccgtg gggatgtctc
     1381 agacaacgag cccggccgcg ttcatctacg cctcgatcga cgccgcgcgc tcgcagatgg
     1441 ccctccgtgg ccacgagctg ctcgacaagg cgctcgaact cgcccacgag gcacgggagc
     1501 gacttcgggc gattcccggg ctgaccgtcg tcgatggcga gacgctgggc gtgactcgat
     1561 atgacccgac caagttcgtg ctcgatgtcg cggggatggg tatcactggc ttcgaagccg
     1621 aggaggaact gcggacgcgc ttcttcatca acccggaggc gagcgatctc gtctcagtcg
     1681 tctgcttcgt gacgatcggt gacacgcgga cgtcgatcga ccggctggtg acagccttcg
     1741 agacgctgag caacgagaac cggggcagac ctagaatcga tacccatctc cggtcatcgg
     1801 gctcggtcat tcggcccggc atccaggcaa tgaccccgcg cgacgccttt ttcgcgagga
     1861 gcagggcggt cccgttgcgg gaagcggccg gcgagatcag cgcggacctc atcattccct
     1921 acccgccggg catcccggtc atcgcgcccg gtgacgtcat cgaagcacac aaactcgact
     1981 acctggagca cggtgtccag gccgggttct acatctcggg cgccgccgac cagaccctcg
     2041 ccacaatcag aatcgtttca gcccaccctt aaccgaatgc cttttggagg cgggagcggg
     2101 cttcggcgat cgtttccagg cgcttggcga agcagaagcg ggcgagcagt ggagcagttt
     2161 cggcgtccag atagaaggcg gatggcggca ccgctgccac gccgtggttc gagaccagcc
     2221 accggcagaa ctcgacatcc gactgaaacc cgaggtccga gatgtcggcc atcaggaagt
     2281 aactgccctt gatcggcagc gccgggattc cagcctcgga gagcgcgtcg tccagcgcct
     2341 gccgccgcgc gtcgtactcg gccgcgagct gcgggtagaa atcgcgctcc tcggcctgct
     2401 cgatcgcctc ggccatcgcc tcctggaagg gagtcgcgct ggcaaaggtc acgaactgat
     2461 ggaccgcccg gatcgccgcg ttcagcggtg cggggccgat cgcgtatccg atcttccagc
     2521 cggtcatgct gaaggtcttg ccggtcgagt tgatcgtcag cgtccgctcc cacatcccgg
     2581 gcaacgtcgc gatcgggata tggcaggcgc cgtggtagag gatacggtca tagacctcat
     2641 cgctcagcac cacgaggtcg tgctcccgcg ccagatcggc gatgaagtcg atctcctcgc
     2701 gggtgaagac cttgccggtc gggttgtgcg gcgtgttcag gagcagcact cgcgtcctgt
     2761 cggtgatcgc tgccttcaac tcgtcgcgat cgaacgacca atcgggcgga ttcagccgaa
     2821 cgggacggaa tgtgcctccg gccatcttcg tcgaagtcgg gtaggagtcg tagaacggct
     2881 cgaacgcgac cagctcatcg ccgggtccga tgaacgcctg gattgcgtcg aagatgactt
     2941 cggtcgctcc cgatgcgacc gtgacatggg catccggatc gatgtcgagg tcgaacctgc
     3001 gcccgaactc gttggcgatc gccttgcgca gacgcggatg accgtgctag acggcgtact
     3061 ggttgagatc agcgtcgatt gcccgcttgg ccgcctcctt gacgaagtcc ggcccggcga
     3121 aatcgggaaa gccctgtccg aggttgacgg cccgatgctg gaccgccaac cggctcatct
     3181 cggcaaagac agaggttccg aaagagcgga ccttctccgc tgcgtatcga tccatttcgg
     3241 ctcaggccgc gtcgaaggcg cccgaggcgg cgatcgcctc cagctcggac accgtctcat
     3301 tgaagaccgc catgcacgcc tcgattgggt ccggcgtggt tagatcgaca cccgccgcct
     3361 tgagcaacgg cagcgggtag tccttgccgc cgctctcgaa caggcgcatc acccgctcga
     3421 ccgcagccgc accctcgtcc ctgacctgct tggcgagcga gatcccggca gaaatgccgg
     3481 tcgcgtactg gtagacgtag aagctcatgt agaagtgcgg cacgcgggac caggagatct
     3541 cgccgcgctc atcgacattc atgcccggcg agtaggttcg gtagaggtcg ccatagagca
     3601 cgttgagctg ctggagtgtc aacggctgct gcgcctcgac gatccggtgc gtctcgcgct
     3661 cgaactcggc gaacattgtc tggcggacgt gcgtgcccat gatctggtcg gcgaaccgat
     3721 tcaggatcgc aaagcgctcc atcgcctttt cggccgggat tttgctcagc agatgccagg
     3781 tcaacaacac ctcgttcagg gtcgacgcta tctcggccag gaagatcggg tactgcgcgt
     3841 cctggtacgt ctgcgatcga tccgccaggt aggtgtgcat ggcgtgaccg gcctcatgcg
     3901 cgagcgtgaa gacgtccgag agcgtgccat tccagttcat caggatcaca ggatgcgacc
     3961 cgtacgcgcc ccacgagtag gcgccagacc gcttgccctt cgtctcgtgg acgtcgaccc
     4021 agcggccgga cgtcaacccc tcgcgcatga tcgagacgta ctcgtcaccg agcgcggcca
     4081 ggccttcgca gacgatctcg accgcctcct gccaggagta gtggacgacc gggagcgtcg
     4141 agagcggcac ccaggtgtcg tagagctcga gctgatcgac accgagcagt cttcgccgca
     4201 gctcgagata ccgtcc
//
LOCUS       DATAOZ010000308         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9071496, whole genome shotgun sequence.
ACCESSION   DATAOZ010000308 DATAOZ010000000
VERSION     DATAOZ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9071496"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VHR64_08045"
     CDS             <1..238
                     /locus_tag="VHR64_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3302816.1"
                     /translation="VPTGAQIMTGPTIVGKIVVVASGQTLYGLDLATGAQVFTYATGY
                     TIQIAPTIVNGVIYIGGRDGYLDAIVGDATKPSG"
     gene            complement(235..>2887)
                     /gene="ppc"
                     /locus_tag="VHR64_08050"
     CDS             complement(235..>2887)
                     /gene="ppc"
                     /locus_tag="VHR64_08050"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX3302817.1"
                     /translation="AGQDAFALEEEVRALGKEFRSGHEDVATQLAAVIRDVSIDDAEM
                     LIRAFTNYFQLVNLCEDNERIRRLKRRERENAPKPRRGSIREAVHILRDRGVTAGQLQ
                     ETLDQAQVKLVLTAHPTEARRRTTIDKQARIFRVLRDFDERGALPAELSRARTRLGST
                     IAELWGSNEIRAVQPTVLDELHAGLIHFRSTIVQTIPLIYRDLEEAIEEAYPGAGIAV
                     PSFLTFGTWVGGDRDGNPNVTPELTREALRILHTSALDALDGRMLELAGRLSLSTHVA
                     GDAPLLRGILDEYSDMFPDLAADLRHRNADELYRQAVTLMRERVRAARRDQPNGYQRA
                     SELLADLRTVAESLQNQGERHVLGGDLHDVIRHVETFGFHLAAMDIRDHASRHEATIA
                     ELFRMWGIAENYASLPEEQKCALLEREINDPRPIIPRLTDRFQAITQEVIATFLLIRD
                     QIREGDPAALGSYVISAAETPSDVLEVLLLMKESDLSEAGGSGAVMPIAPLFESLSSL
                     SGATGIMERLIDSGPYRAALASWGDEQEVMIGYSDSNKEIGYLASTWALFEAQTKLTE
                     LFDQQGIHGMFFHGRGGSIGRGGGPTNEAILAQPQGTIQGRIKLTEQGEVISARYSVP
                     EIAIREFELVVGAVLVASFGGLSTVAAVDSAPFRDAMRTLADVSRETYRDLVYGDPDF
                     VDFFQQATPIREIAELKLGSRPARRTSSTRIEDLRAIPWVFSWTQARMVLPGWYGMGA
                     ALEQGCADFGLDLLQHMYRDWPFFATTIGNAEMALSKADMPIAERYVAMVEPEPVRDR
                     IWGRIRAEYDRTERMILLISGQSHLLEHDPVLRRSIERRNPYVDPLSFLQLELIQRLR
                     STEDAESLIRPVLLTINGIAGALKNTG"
BASE COUNT          525 a          946 c          922 g          494 t
ORIGIN      
        1 tgtgccgacc ggcgcccaga tcatgaccgg gccgacgatc gtcggcaaaa tcgtggtggt
       61 ggccagcggc cagacgctct acggcctcga cctcgcgact ggcgcgcagg tcttcaccta
      121 cgcgaccggg tacaccatcc agatcgcgcc aaccatcgtc aacggcgtca tctacatcgg
      181 cgggcgcgac ggctaccttg acgcaatcgt cggagacgcc accaaaccct ccggttaacc
      241 cgtgttcttg agggccccgg cgatgccgtt gatggtcaga aggaccggcc ggatcagcga
      301 ctccgcgtcc tccgtggacc gcaggcgctg aatcaattcg agctgcagga acgacaaggg
      361 atccacgtac ggattgcgcc gctcgatcga gcgccgcaac accggatcgt gctcaagcag
      421 gtgcgattgc cccgagatca gcaggatcat gcgctcggtg cggtcgtact ccgcccggat
      481 cctgccccag atgcggtctc gcaccggctc cggctcgacc atcgcgacgt agcgttcggc
      541 aataggcatg tcggccttgc tgagcgccat ctcggcgttg ccgatcgtgg tcgcgaaaaa
      601 cggccaatcc cggtacatgt gctggagcag atcgagtccg aaatcggcgc agccctgctc
      661 gagcgcggcg cccatcccat accagccggg cagaaccatc ctcgcctgcg tccaggaaaa
      721 gacccacggg atcgcgcgca agtcctcgat ccgggtcgac gatgtgcgcc gagccggtcg
      781 cgagccgagc ttcagctcgg cgatctcgcg gatgggcgtg gcctgctgga agaaatcaac
      841 gaagtccggg tcgccataga ccaagtcgcg gtacgtttcc cgggagacat cggccagcgt
      901 ccgcatcgca tcgcggaacg gggccgaatc gaccgcagcc acggtactga gaccaccgaa
      961 actggcgacc agcacggcgc cgacgaccag ctcgaactca cggatcgcga tctccggcac
     1021 ggagtaacgg gcggagatta cctcgccttg ctcggtcaac ttgatgcggc cctggatagt
     1081 tccctgtggc tgggcgagga tggcctcatt agtcggtcca ccaccgcgac cgatcgagcc
     1141 accgcgaccg tgaaagaaca tcccgtggat accctgttgg tcgaacaact cggtcagctt
     1201 cgtctgtgct tcgaaaagtg cccacgtcga ggcgaggtaa ccgatctcct tgttcgagtc
     1261 cgagtagccg atcatcacct cttgctcgtc gccccacgac gcgagtgccg cgcggtaggg
     1321 accggagtcg atcaggcgtt ccatgatccc ggtcgctccg gagagggacg agaggctctc
     1381 gaaaagcgga gcgattggca tcacggctcc gctgccgccc gcctcggaca ggtccgactc
     1441 cttcatcagg agcaggactt cgagcacatc ggatggcgtc tcggccgccg agatgacata
     1501 gctgccgagc gcggccggat cgccctcgcg gatctggtcg cgaatcagca ggaatgtcgc
     1561 gatcacctcc tgcgtgatcg cctggaaccg gtcggtgaga cgcgggatga tcgggcgcgg
     1621 atcgttgatc tcccgctcga gtagcgcgca tttctgctcc tcggggagac tggcgtagtt
     1681 ctcggcgatc ccccacatcc ggaagagctc ggcaatcgtc gcttcgtgcc tcgaagcgtg
     1741 atcgcgaatg tccatcgccg cgaggtgaaa gccgaacgtt tcgacgtggc ggatgacatc
     1801 atgcagatcg ccgccgagca cgtgccgttc gccctggttt tgcagcgact cggcgaccgt
     1861 gcgcaggtcg gcgagcaact cggatgcccg ctgatatccg ttcggctggt cacgtcgtgc
     1921 cgcccggaca cgctcgcgca tcagcgtgac tgcctgccga tagagttcgt cggcattgcg
     1981 gtggcggaga tcggcggcga ggtcgggaaa catgtcgctg tattcgtcca gaatcccgcg
     2041 cagaagcggc gcatccccgg ccacgtgagt cgagagcgag agacgacccg cgagctcgag
     2101 cattcgccca tccagggcat ccagcgcgga cgtatgcagg atccgaagcg cctcgcgagt
     2161 caactccggc gtgacgttcg gattgccatc gcggtcgcca ccgacccacg tcccgaaggt
     2221 caggaacgac gggaccgcga tcccggcgcc ggggtacgcc tcttcgatcg cctcttccag
     2281 atcgcggtag atgagcggaa tcgtctggac aatcgtcgag cggaagtgga tcaacccggc
     2341 atgcagctcg tcgagcacgg tcggctgaac cgcccggatc tcgttgctgc cccaaagctc
     2401 ggcgatggtc gacccgagtc gcgtgcgggc acgggatagc tcggccggga gggcgccgcg
     2461 ctcatcgaaa tcgcgcagaa cacggaagat gcgcgcctgc ttgtcgatcg tggtgcggcg
     2521 acgcgcctcg gtcgggtgcg ccgtcagcac cagcttgacc tgcgcctggt cgagtgtctc
     2581 ctgcaattga ccggcggtta cgcctcgatc gcggaggatg tgaacggcct cgcggatcga
     2641 gccccgtcgg ggcttgggcg cgttttcacg ttcccggcgc ttgagccgcc ggatccgctc
     2701 gttgtcctcg cagagattga cgagctgaaa gtaattggtg aaggcgcgga tcagcatctc
     2761 ggcgtcatcg atcgaaacat cacggatgac tgccgctaac tgagtcgcaa cgtcctcatg
     2821 cccggaccgg aactccttgc caagtgcccg tacctcttcc tcgagcgcga acgcatcttg
     2881 accggcc
//
LOCUS       DATAOZ010000309         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9334639, whole genome shotgun sequence.
ACCESSION   DATAOZ010000309 DATAOZ010000000
VERSION     DATAOZ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9334639"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VHR64_08055"
     CDS             complement(<1..509)
                     /locus_tag="VHR64_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX3302818.1"
                     /translation="MEHSLSNPALRRQYDLAGFGGRVGWGSRPALLVIDMAGAWTSPG
                     EQLGSDLSGVMRSILELLPIARRQRIPIYFTTMAYGTPEEAGDVVCRKLPHTRNMLRG
                     SKRVELAPELHRQPYEPLIEKPRASAFFATNLLALLIAARVDTVIVTGCSTSGCIRGT
                     CESAHNYNF"
     gene            764..2068
                     /locus_tag="VHR64_08060"
     CDS             764..2068
                     /locus_tag="VHR64_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302819.1"
                     /translation="MTKRHRPSLPVVLAAVLVVTASATAMAASEYTPAIPEAGTDIPQ
                     VSVTFGMRPYADNTFYVIGIEKGWFKDVGITIEPEPYGLKTTENQWISILLNQQVDIN
                     SATCSIMLPSYKTTDQLKCTGFAVTFFGSVMLANPKLNLRTLRDYMKGGVALKDGLHQ
                     ALTPLVGKTVYVPAAVSEKEFNEVPFKLAGLDLPNYVTMDDSQMLLLAKSGRLDFMHP
                     GGAPIAQTLLDAGWTPVYDTGQLLEFGPGGVDSPLESLVFNNGWGANADYINHNQTTM
                     MRFASVVFRIFGELKKDPSLYGVYAPYLNSVAGTSLDANGVRRTVENLDPFVPFEQQT
                     KYFIDKNGPEYYGNSMGALIKALETSGTIPAGITPDQVVWAAPMYHELVDYQKKSDAL
                     FERAKTAQLSEPKQSLLAKARQYYGWYDFLDAYRLATAALAD"
     gene            2192..>2965
                     /locus_tag="VHR64_08065"
     CDS             2192..>2965
                     /locus_tag="VHR64_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3302820.1"
                     /translation="MDPFGILGLLVLIAMWWAISQLRLVSPLFLPPPGGVALTIAENF
                     FSSPYLANYHLGDGGLLASLSYTVSNVIIALAISCLVGIALGLASARIGLLRAVLDPV
                     VLTAGTIPILVTAPFFLIWFGTSRTAQIALLILYDVTILYLFAQRAAANLDPIYVAAS
                     RTLGATPGRIILDVYLAGTLPEVFGGIRIAMAGAWGLEAFSELLGAPHGIGRVIQAMG
                     SGMDTQTMVAAILTLAIVAVGFDALIAGVFGFITRWRRPA"
BASE COUNT          514 a          987 c          904 g          560 t
ORIGIN      
        1 tgaaagttat agttatgcgc gctctcgcag gtgcctcgga tgcagccgct ggtgctgcag
       61 ccggtcacga ttaccgtatc gacgcgcgcg gcgatcagca gcgcaagcag gttggtggcg
      121 aagaacgccg aagcccgcgg cttctcgatc aacggttcat aaggctgccg atggagctcc
      181 ggcgcgagct cgacacgttt gctcccccgg agcatgttcc tggtgtgcgg cagcttccgg
      241 cagaccacgt cgccggcttc ctcgggcgtc ccgtacgcca ttgttgtgaa gtatatggga
      301 atccgctgcc ggcgcgcgat cggcagcagc tcgaggatcg atcgcatgac cccgctgaga
      361 tcggagccga gctgctcgcc ggggctggtc caggcgccgg ccatgtcgat cacgagcaga
      421 gcgggccggc tgccccagcc cacccggcct ccgaaaccgg cgaggtcgta ctggcggcgg
      481 agcgcggggt tgcttagact gtgctccatc gtcttaccct cccccttcag tcagagatcc
      541 gtcgggaaca caaaccagct cgacccttag gcgtcaacac gtccggcagg ttcgacgaac
      601 ggccgcgcat gcttgacctc gtcgatcagc cctgttagcg cttttcgcgc agcccaagcc
      661 atcccatgct ttcggcatat ccaaagccat cccatcatgg ctatcggctc cggcggcagc
      721 cgccaaaatg tgaaaagtca cgaaagcgcg gagggggagt gcgatgacga agcgacaccg
      781 accgtccttg cccgtcgttc tggcggccgt gctggtggtc acggcctctg cgaccgccat
      841 ggccgccagc gagtacaccc cggcgatccc cgaggccggg acggacatcc cacaggtcag
      901 cgtgaccttc ggcatgcgcc cctatgccga caacacgttc tatgtgatcg gcatcgagaa
      961 gggttggttc aaggacgtcg gcatcacgat cgagcccgag ccctatggtc tcaagaccac
     1021 cgagaaccag tggatctcga tcctgctgaa tcagcaggtc gacatcaact cggcgacctg
     1081 cagcatcatg ctgccgtcgt acaagacaac ggatcaactg aagtgtacgg gtttcgctgt
     1141 gaccttcttc ggttcggtga tgctcgccaa cccgaagctc aacctgagaa cgctccgcga
     1201 ctacatgaag ggaggggtcg ccctcaagga cggcctgcac caggcgctta cgccgctggt
     1261 cggcaagacg gtctatgttc ccgccgccgt ttcggagaag gagttcaacg aggtcccgtt
     1321 caagctcgcc gggctcgatc tgcccaacta cgtgacgatg gacgactcgc agatgctgct
     1381 gctcgccaag agcggccgcc tcgatttcat gcatcccggc ggcgcgccga tcgcgcagac
     1441 gctcctcgat gccgggtgga ccccggtcta cgacaccggc caattgctgg agttcggtcc
     1501 tggcggcgtc gattccccgc tcgaatcgct ggttttcaac aacggctggg gcgccaacgc
     1561 cgactacatc aaccacaacc agaccacgat gatgcgcttc gcctccgtgg tcttccggat
     1621 cttcggcgag ctgaagaagg atccgtccct ctacggcgtc tacgcgcctt atctgaacag
     1681 cgtcgccggc acctcgctgg atgcaaacgg cgtccggcga accgtggaga acctggatcc
     1741 gttcgtgccc ttcgaacagc agaccaagta cttcatcgac aaaaacggcc cagaatacta
     1801 cggcaactcg atgggtgctc tgatcaaggc gctggaaacc tccggcacga ttccggccgg
     1861 catcacaccg gaccaggtcg tctgggcggc tccaatgtat catgagctgg tcgactacca
     1921 gaagaaatcc gacgccttgt tcgagcgcgc caagaccgct cagctttccg aaccgaagca
     1981 gtctctgctt gccaaggcgc gccagtacta cggctggtac gatttcctgg acgcctaccg
     2041 gttggcgacc gcagcgctcg cggattaggt ctgggtcgtc acgggggcga tggtgatctg
     2101 aaactggcaa gcgctgcggc ggcgcgcgcc ggctagaatg gtggaaaacc ggatcacggt
     2161 ccgaccggca tcggcagccc ggcgtctcgc cgtggacccg ttcgggatcc tcgggctgct
     2221 ggtcctgatc gccatgtggt gggcgatctc ccaactgcgg ctcgtgtcgc ccctgttcct
     2281 gccgccaccc ggcggtgtcg cgcttacgat cgccgagaac ttcttctcgt cgccctacct
     2341 tgctaactat cacctcggcg atggcgggct tctggcgagc ctctcgtata ctgtgtcaaa
     2401 cgtgatcatc gcgctcgcga tctcctgcct ggtcggtatc gccttggggc tcgcgagcgc
     2461 ccgtatcggt ctcctgcgcg cggtcctcga ccccgtcgtg ctcaccgccg ggacgattcc
     2521 gatcctagtg accgccccgt tcttcctgat ctggttcggt accagccgaa ctgcgcagat
     2581 cgccttactg atcctctacg acgtcacgat cttgtatctg ttcgctcagc gcgccgccgc
     2641 caatctggac ccgatctacg tcgccgccag ccgaacgctc ggagcgaccc cggggcggat
     2701 catcctcgac gtctacctcg cgggtacgtt gcccgaagtg ttcggcggga tccgcattgc
     2761 gatggcgggg gcatggggcc tcgaggcctt ctccgagctg ctcggcgcgc cgcacggcat
     2821 cggccgagtc attcaggcca tggggtcggg gatggacacc cagacgatgg tggcagcgat
     2881 cctgacgctc gcgatcgtcg cggtcggctt cgacgcgctt atcgccgggg tattcggttt
     2941 catcacccgc tggcggcggc cggct
//
LOCUS       DATAOZ010000310         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3022276, whole genome shotgun sequence.
ACCESSION   DATAOZ010000310 DATAOZ010000000
VERSION     DATAOZ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022276"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VHR64_08070"
     CDS             complement(<1..503)
                     /locus_tag="VHR64_08070"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEX3302821.1"
                     /translation="MKRLVDTAKIGIVTDRHVARHYLKPTVDSLKHSGLDPVPIILPP
                     GERTKTLTTVGRILDILAAEKFERRSVVLALGGGVIGDVAGFAASIYQRGIPFVQVPT
                     TLVAQVDSSVGGKTGVDHRLGKNLIGAFHQPRAVIIDPFMLHTLPRREWIAGLAEVIK
                     YGIIADEA"
     gene            408..629
                     /locus_tag="VHR64_08075"
     CDS             408..629
                     /locus_tag="VHR64_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302822.1"
                     /translation="MLQRVHRRLQIVPGDMPVGDDPDFRRVDESLQAVTERSARARLK
                     DDVVAPLAEMDGNPLAGHRCHRHGTGDPL"
     gene            complement(771..>1782)
                     /gene="pilQ"
                     /locus_tag="VHR64_08080"
     CDS             complement(771..>1782)
                     /gene="pilQ"
                     /locus_tag="VHR64_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX3302823.1"
                     /translation="DEEARAKDAKIKAEDLVDRVFYVRNLQAQELMTALRQNLSPRGL
                     MQISQGTNALVVRDTETKMAVLKQLIEGLDLEVPQVQIEARIVQADTVYARGLGVQWG
                     IQNSDFGPNTFSAVGNLTGPFAPVAGTGGTTIPRDFLVNLPAQVAGLPAVPGIGYTFG
                     KLAPGFALDLRLSAGELLGLAKVIAAPKITTLDKREAKISQGESIPFQTTSLQGTQTT
                     FVDANLELNVTPQITSRDPREVGKQIMMKVRATRNAVGARSNPAGPSIDRREANTQVI
                     VRDGETMVIGGVFIDTQNNNVQGVPYLSRIPVLGWLFKNKSESVSKQELLIFLTPSIV
                     KA"
BASE COUNT          336 a          545 c          544 g          357 t
ORIGIN      
        1 gcttcgtcgg cgatgatgcc gtatttgatc acctccgcca gacccgcaat ccattcacgg
       61 cgcggcaggg tgtgcagcat gaacggatca atgatgaccg cccggggctg atgaaacgcc
      121 ccgatcaaat ttttgcccag acgatgatcg acgcctgttt ttcctccgac gctggagtcc
      181 acctgcgcga ccagcgttgt cggcacctgc acaaagggga tgccccgctg gtagatcgag
      241 gcggcgaacc cggcgacatc cccgattacg ccgcctccga gcgccagcac caccgatcgg
      301 cgttcaaact tctcggcggc cagaatgtcg agaattcgtc cgacggtcgt aagcgtcttc
      361 gtgcgctccc ccggcggcaa gatgatcggc acaggatcga gtccggagtg cttcagcgag
      421 tccaccgtcg gcttcaaata gtgccgggcg acatgccggt cggtgacgat cccgattttc
      481 gccgtgtcga cgagtcgctt caggcggtca ccgagcgttc ggctcgagcc aggctcaagg
      541 acgatgtcgt agctccgctc gccgagatgg acgggaatcc gttggccggt catcggtgtc
      601 atcgacatgg aacgggtgac ccactttaag ggagcgccgt ggccgggggc cgattggcgg
      661 caaatcgtgg cgatggtagc atactcgcca tgaaaagagc atagcggccg gaaggacgaa
      721 cgcgacgtcg aagccggccg gcaacccgca cggaggctgc cggcaatcct ctaggctttg
      781 acgatggagg gagtcaggaa gatgagcaac tcttgcttcg agacgctttc ggacttgttc
      841 ttgaacaacc acccgaggac cggaattcgc gagagatagg gcacgccctg cacgttgttg
      901 ttctgcgtgt cgataaagac accgccgatc accatggtct ctccgtcgcg aacgatgacc
      961 tgcgtgttgg cttcccggcg atcgatactg ggacctgccg ggttgctcct cgcgccgacc
     1021 gcgttgcgcg tcgcgcgtac cttcatcatg atttgtttgc ccacttctct cgggtcacgc
     1081 gaggtaattt gcggcgtcac gttcagttcg agattcgcgt cgacgaacgt cgtctgggtg
     1141 ccttgcagcg aggtggtctg gaaaggaatg gattccccct gcgagatctt ggcttcgcgc
     1201 ttatccagcg tcgtgatctt gggcgcggca atgaccttgg ccaagcccag taattctccg
     1261 gccgacaacc gtaagtccaa tgcgaagccg ggagcgagct tgccaaacgt gtacccgatg
     1321 ccgggaaccg ccggaagacc cgcaacctgg gccggcaaat tcacgaggaa gtctcgcgga
     1381 atcgtggtcc cgccagtccc ggcgaccgga gcgaagggac cggtcaggtt gcccactgcc
     1441 gaaaatgtgt tgggaccgaa atcggagttt tggatgcccc actgcacccc taacccgcgg
     1501 gcatacacgg tgtccgcctg cacgatgcgc gcctcgatct gtacttgagg cacctcgaga
     1561 tcgagccctt cgatcaactg cttgaggacc gccatcttgg tttccgtgtc cctgacgacc
     1621 aaggcgttgg tgccctggct gatctgcatc aagcctcgcg ggctcaaatt ctggcgcagt
     1681 gcggtcatca actcctgggc ctgcagattg cgaacgtaga agacgcggtc gaccaaatct
     1741 tcggccttga tcttggcgtc cttggcccgt gcttcttcat ct
//
LOCUS       DATAOZ010000311         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8285618, whole genome shotgun sequence.
ACCESSION   DATAOZ010000311 DATAOZ010000000
VERSION     DATAOZ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8285618"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..649
                     /locus_tag="VHR64_08085"
     CDS             134..649
                     /locus_tag="VHR64_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX3302824.1"
                     /translation="MVNPRVLEYETLIALSQLKLHETATVTMALKNVAMSYPAADYYG
                     HPRSTRKHENCFFADMHSFIAAMARRFPIDLAITVGHPVMIATGPLGGHAVETGLVIA
                     STDPVAADVVGARLLGFTPQAVRHLWEAGRLGLGETDVDKLEFPVLSLRQAIEAFTEA
                     AYGERLTFDHA"
     gene            773..1312
                     /locus_tag="VHR64_08090"
     CDS             773..1312
                     /locus_tag="VHR64_08090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3302825.1"
                     /translation="MLTVYAFGDSILDCGHYNPYGANSGQLLVRNDDRLFPDFRGQDL
                     MPRGPTCLEHRAQDGATVRSLPLQAVDIRVHGPVLALVTIGGNDLLGGLIADRGPGVE
                     AFAQALDDFLVSLPIRPILIGNVYDPTFGDDHRNFLSIEPAVARANLGRINAMLAQPA
                     THYGQLVDLHAHFLTGDPS"
BASE COUNT          262 a          554 c          491 g          280 t
ORIGIN      
        1 cgggtggccg gattgatggc ggtggtcgag caggacgggg cggaccttct tcgaccacaa
       61 ccgtccacca ttcgtctcgg ttggtcttga ctatgcacct gaccgggacg tcggtgggcc
      121 gcagcaggcg atcatggtca acccgcgggt gctcgagtac gagacgctca tcgcgctgag
      181 ccaactgaag ctgcacgaga cggcgactgt cacgatggcg ctcaagaacg tcgcgatgtc
      241 ctacccggcc gccgactact acggccaccc gcgctcgacc cggaagcacg agaactgctt
      301 cttcgccgat atgcactcct tcattgccgc gatggcccgg cgttttccga tcgacctggc
      361 gatcacggtc ggccaccccg tcatgattgc gaccggcccg cttggcgggc acgccgtcga
      421 gaccgggctg gtgatcgcca gcaccgaccc cgtggcggct gacgtggtcg gcgcccgcct
      481 acttggcttc accccgcaag ctgtgcgcca cctttgggaa gccggccgcc tcgggctcgg
      541 cgagaccgat gtcgataagc tcgagttccc ggtcttgagc ctgcgccagg ccatcgaagc
      601 cttcaccgaa gcggcctacg gcgagcgcct gacattcgac cacgcgtaat cctgggctgg
      661 cgtaggcatc caatgccagg ctctcgcgac gcgcggccct cgccgccggc gccgcgctcg
      721 ccattagtgg cactgcctgc gatcggctgc catggctggt gaaagagggg ggatgctgac
      781 ggtatatgcc ttcggcgact cgatcctcga ctgtggtcac tacaatccgt acggggcaaa
      841 ctccggccag ctgctggtcc gcaacgacga ccggctcttc ccggacttcc gcggtcaaga
      901 tctcatgccg cgaggcccga cttgcctgga gcaccgcgcc caggatggcg ccaccgtccg
      961 gagcctgccg ctgcaggccg tggacattcg cgtccatggc ccggtcctgg cgctcgtcac
     1021 catcgggggg aatgatctgc ttggcggcct gatcgccgac cgcggcccgg gtgtcgaggc
     1081 cttcgcgcag gcactggacg atttcctggt cagcttgccg attcgcccga tcctaatcgg
     1141 caacgtctac gacccgacct tcggtgatga ccatcgcaat ttcctgtcga tcgagccggc
     1201 tgtcgcacgc gccaatctag ggcggatcaa cgcaatgctg gcacagcccg ccacccacta
     1261 tggccaactg gtcgatctgc acgcccactt cctgaccggc gatccatcct gaccattgat
     1321 tccatacggc atcaccacgc agccccatgt ccgccaacac tgcaccgagg cgattcggtc
     1381 gaaccggcca gcctcggatt tttgctcgac ggattgcgca tcgctagcct ccaggttgag
     1441 ccgaagcgca acgtgacggg gaggctccgc cctcggcacg ggtcttgatc caacagccgg
     1501 agacatcgaa cgcctcggtc ggtgacccgc tctccggccg gacgcccggc gtagcacggt
     1561 ggaggagctg aggtgccatc tagatcg
//
LOCUS       DATAOZ010000312         4550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5916753, whole genome shotgun sequence.
ACCESSION   DATAOZ010000312 DATAOZ010000000
VERSION     DATAOZ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5916753"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..819
                     /locus_tag="VHR64_08095"
     CDS             136..819
                     /locus_tag="VHR64_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3302826.1"
                     /translation="MKILLIEDEPAIARMIERGLSPHGFDIIAVESGDDPEVEFSVDL
                     VDLVLLDIHLPGRDGISVLADIRRLRRDLPVIMLTALDDLDTKVSALNGGADDYVTKP
                     FAFEELIARMRAITRRVDQRAAGAIEFGDLRIDLLSHRVSRLNQIVELSRREFALLEY
                     FARNPGRVLSRQQILTAVWDYDFEGESNVVDVYVRYLRQKLEQLGVARVFTTIRGSGY
                     RFDPPPARA"
     gene            1065..1874
                     /locus_tag="VHR64_08100"
     CDS             1065..1874
                     /locus_tag="VHR64_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302827.1"
                     /translation="MTKRLVAYSRILLPLGLALMLAVVAVTTTLARSRVQSGSETKSA
                     QDADGTIPPVPRPVRVYAGACGTLSDVVWPLNDLISPDGQDGGSKDADRTEYSFTANL
                     PMTIAAMLSGSYAINVHESQDALDHSLTCGNVGGVPDAVGTLVIGLRNQDGTDVTGIA
                     VLSPSPSDPGMTFVSVFISGGGLGDAVGTIGANPPVANNPPVDDTPPVVDNPPVDGNP
                     PPVDNGDDGDDHEDDGVDDGHDGSDDYRGDTKEDSGDDHSGEDNSGPGSGD"
     gene            2038..2649
                     /locus_tag="VHR64_08105"
     CDS             2038..2649
                     /locus_tag="VHR64_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302828.1"
                     /translation="MQGDDMLQRLMMLTRMGVTFSWALALAGGWGMISHSDAAAGGSR
                     VSTQISARFDDGEDDSEHEVDDHSGHDDSEDVSDDHGDDGEQVNGDHGGDNEVDDAND
                     DHGDAAEDDQGDANNDDQGEDVNDDHGGDSVDDSTGDSTDDSVNDNADDSADDSADDE
                     SDDDADDVNVEIDQNGNGTIDFDLDGDGVADFTVVIGKDGGFR"
     gene            complement(2738..3127)
                     /locus_tag="VHR64_08110"
     CDS             complement(2738..3127)
                     /locus_tag="VHR64_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3302829.1"
                     /translation="MHKERQRIASGSPFEKTIGFSRAMRVGNRVIVAGTAPIWPDGSC
                     DPDPEVQARRCLEIITTALAEAGAGPEHVIRTRQYLTDPGDADAVGRAHGAMFAEVRP
                     ASTMIVVAALLDPRWKVEIEAEAIIDD"
     gene            3841..>4550
                     /locus_tag="VHR64_08115"
     CDS             3841..>4550
                     /locus_tag="VHR64_08115"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEX3302830.1"
                     /translation="MRSVFSALRTAMAVMAVALVLSPFVGSILPARAASPQLKVDPGH
                     GLPGSKVTVSGTGFTAKKSGSAWWDENGDVEVDFTTDANGAFQAQIDVPNLDAGNQDI
                     GVSIGDYDGSVTFVVDSGSAPTQPTQAPTQAPAPGDQTTPEATPTKTATAEPTKTTTP
                     APNESTGSTIWVSTSGSDSASGSSSAPFKTLQKALDVVKPGMTINLKSGKYSLSSTAN
                     SKTNGSSSAPITIQSAPN"
BASE COUNT          842 a         1475 c         1428 g          805 t
ORIGIN      
        1 ggcggcgaca agaccccacg gtcttgtccc gctcccgcga acgcgtcgca tcgctgccgt
       61 attcctgccg gttccgtgac tcattggcca gccaactacc gtcgcctctc aagcgcgcgg
      121 tctatcctgt gtcgcatgaa gatcttgctc atcgaagacg aaccagccat cgcgcggatg
      181 atagagcgtg gcttgtcgcc gcacggcttc gacatcatcg ccgtcgagtc cggcgacgat
      241 cccgaggtcg agttctcggt cgacctcgtc gatctcgtgc tcctcgacat ccacctccct
      301 ggtcgggatg gtatcagcgt gctggccgat attcgccgtc tgcgacggga ccttccggtg
      361 atcatgctta ccgcgctcga tgacctcgac acgaaagtct cggccctgaa cgggggtgcg
      421 gatgactacg tcaccaagcc gttcgcgttc gaggagctga tcgcgcggat gcgcgcaatc
      481 acgcggcgag tcgatcagcg ggccgcgggg gcgatcgagt tcggggatct ccgcatcgat
      541 ctgctctcgc accgggtctc gcggctcaat cagatcgttg agctctcccg gcgcgagttt
      601 gccctgctcg agtacttcgc gcgcaatccc ggccgggtgc tttcgcgcca gcagatcctg
      661 acggccgtct gggattacga cttcgagggc gagtcgaacg tggtcgatgt ttacgtgcgc
      721 tatctgcggc aaaaactgga gcaactgggc gtggcgcgag tcttcaccac gattcgcggc
      781 agcggctacc ggttcgatcc gccgccggcg agggcctgat cgcacccgac cccgccggcg
      841 acccagcccg accgggggat gagaaagttc tcatccaggt ctcatatccg cctcacgatc
      901 cccgcatatc gtggctgaaa ggcaagccgg acgcatccgg tctcgcgctg tggccgaaat
      961 ctcggtgccc ggcgcgagcg gtatcgcaca ggtttgcgga atctggtcaa tctgttcgtc
     1021 cgtcgtcgtg gtcagccaca gcggaccttg cgcgagtgaa cgcaatgacg aaacgccttg
     1081 tcgcctattc ccggatcctg ctcccgctcg gcctcgccct gatgctggcc gtcgtggcag
     1141 tcacgaccac gcttgcgcgt agtcgggtcc agtccggctc ggaaacgaag agcgcacagg
     1201 acgccgacgg caccattccg ccggtcccgc gcccggtccg cgtctacgcg ggcgcttgcg
     1261 gcacgttgag cgacgtcgtc tggcccctga acgacctgat ttcgcccgat ggccaggacg
     1321 gcggatcgaa ggacgcggat cgaaccgagt actccttcac cgccaacctg ccgatgacga
     1381 tcgccgccat gttgagcggc agttatgcga tcaatgtcca tgagagccag gacgccctcg
     1441 accattcgct gacctgtggc aacgtgggtg gcgtgcccga tgcggtcggc acactcgtga
     1501 tcggcttgcg gaaccaggac gggaccgacg tcaccggcat cgccgtgctc tccccgagcc
     1561 catcggatcc cggcatgacc ttcgtctcgg tcttcatttc cggcggcggc ctgggcgacg
     1621 cagtcggcac gatcggcgcc aatcctccgg ttgcgaacaa tccgccggtc gacgacaccc
     1681 cgcccgtggt cgacaacccg ccagtcgatg gcaatccgcc cccggtcgac aacggggatg
     1741 atggcgacga tcacgaggac gacggcgtcg atgacggcca cgatggtagc gacgactacc
     1801 gcggcgacac caaagaggac agcggcgacg accacagcgg ggaagacaac agcggccccg
     1861 gcagtggcga ttgagcgccg cggctgatga gaactttcac atccgggtct cacagttctc
     1921 tcatcgtgcc ctcatagagt gcggtcagtg aacgacctgg cactgcccgc cgggtgacga
     1981 tctgaaccgc ccatgggtgg cggtggctgg tataagtggc ggcgcggatg tccgcgaatg
     2041 cagggggacg atatgttgca gcggttgatg atgttgaccc ggatgggcgt taccttttcg
     2101 tgggcgctgg cgctcgccgg tggctgggga atgatctcgc acagcgatgc ggcggcgggt
     2161 ggctcccgcg tctcgaccca aatttcagct cgattcgacg acggcgagga tgacagcgag
     2221 cacgaggtcg acgaccacag tggccacgat gactccgagg atgtcagcga cgatcacggc
     2281 gacgacggcg aacaggtgaa cggcgaccac ggcggggaca acgaggtcga cgacgcgaac
     2341 gacgatcatg gcgatgccgc cgaagacgat cagggcgatg cgaacaacga cgaccagggc
     2401 gaggacgtca acgacgatca cggcggcgac tcggtcgacg actcaaccgg cgactcaact
     2461 gacgactccg tgaatgacaa cgccgatgac agcgccgacg attcggcgga cgacgagtcc
     2521 gatgatgatg ccgatgacgt gaacgttgag atcgaccaga acggcaacgg tacgatcgat
     2581 ttcgaccttg atggcgatgg cgtggccgac ttcacggtcg tcatcggcaa ggacggcggg
     2641 ttccgctaat cccatttctc tcccctaccc aagtgaatgt gcccgggctc ggctgggagc
     2701 ccgggcacat tcgcgtttgc ggcataacca gccaacgtta gtcgtcgatg atcgcctcgg
     2761 cttcgatctc gaccttccag cggggatcga gcagcgccgc gactacgatc atcgtgctgg
     2821 ccgggcgcac ttccgcaaac atggcgccgt gcgcccgtcc gacggcatcg gcgtcgcccg
     2881 gatcggtcag atactgccgg gtgcgaatga cgtgctcggg cccggcgccg gcttcagcga
     2941 gcgcggtcgt gatgatctcc aggcagcgtc gtgcctgcac ctcgggatcg gggtcgcacg
     3001 atccatccgg ccagatcggc gcggtcccgg cgacgatcac gcggttgccg acgcgcatcg
     3061 cccggctaaa cccgatcgtc ttttcgaagg gcgaacccga cgctatccgc tgacgctctt
     3121 tgtgcattgt gcagtccttc ccgaatcacg aatttccgga atcgtagcgg agccgcttcc
     3181 gtaatgaaga aaaccgcctt cttgcgggcg tattccggcc agtccaagac gagcgccagc
     3241 cacagatgat ttgcgtgagc agatatacgc ctggatgacc ttgacgcacg gccaatgagt
     3301 tcgtacagtc ttgcggtcgc agagggatgt gttgaggtcg ggtggctccc gaccgggcca
     3361 caactggcca cgcatcctga tttcaagagc gacctttgat cacgaaaggg ggacgttgct
     3421 gtggctggcg aacactccac atagttggta catctttgca tcgagctggc tgggcggttt
     3481 ccccgcacac cccgcgtgcc caaacccccg cgaactgccg gcccgatgca cgagaatcag
     3541 cgctctagaa tgctggctgg acttcgacga gcgcttaccc ggcaagaatc caccacataa
     3601 ctgatagcca cgattgcgca ccggtcattt gccaatgtgc tccttcgacg cacgcgcgcc
     3661 ggaacgagtt cgcgcggccc ggataccggc cgaagcatgt caatgcgtca atcgtcccgg
     3721 cgcagtctgg tccgattcac ctgccggcaa acgtatcccc agcctagtgt cacggctcgc
     3781 ttcgccgcgc accatcgcgg acagagatgt gcgccctgac agttggaagg caagatccgg
     3841 atgagatctg tattttcggc actgcgcact gccatggctg tgatggcggt ggcactggtg
     3901 ctttcgccct tcgtgggcag cattttgccc gcacgggcag cttcacctca gttgaaagtc
     3961 gatccggggc atggactccc gggcagcaag gttacggtta gcgggaccgg gttcacggcc
     4021 aagaagagcg gctcggcctg gtgggatgag aacggcgatg tcgaggtcga tttcacgacc
     4081 gatgccaatg gcgcgttcca ggcccagatc gatgtgccga acctcgatgc cggcaaccag
     4141 gatatcggcg tctccatcgg cgactatgac ggctcggtga cctttgtcgt cgactcgggt
     4201 agcgcgccga ctcagccgac ccaggcgccc acgcaggcac cagctccggg cgaccagacg
     4261 acccctgagg cgaccccgac caagacggcc acggctgagc cgaccaagac gaccacgccg
     4321 gctccgaacg agtcgaccgg ctcgaccatt tgggtctcga cctcgggctc cgattcggcg
     4381 tccggatcgt cgagcgctcc gttcaagacc ctgcagaagg cgctcgatgt cgtcaagccg
     4441 ggcatgacca tcaacctcaa gtctggcaag tactcgctct cgagcaccgc caacagcaag
     4501 acgaacggct cgtcctcggc tccgatcacc attcagtcgg ccccgaatga
//
LOCUS       DATAOZ010000313         4723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_914939, whole genome shotgun sequence.
ACCESSION   DATAOZ010000313 DATAOZ010000000
VERSION     DATAOZ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914939"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(496..1596)
                     /locus_tag="VHR64_08120"
     CDS             complement(496..1596)
                     /locus_tag="VHR64_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX3302831.1"
                     /translation="MRQRFLAILMLALVLVGGSMPNTATSAAQAGTPSPFVETEAGRN
                     DAPIPVVLPRDDAPHDVGIEWWYYTGHLFTDSGDRYGFEYVIFKGHRGDVLGYAAHFA
                     ITDNAIGRFSYDQRLGLVPETPPSASGFDLRVGDWQMRGAGGNDHLVASMAEYGIDLT
                     VQTTKQPALHDGDGYYDYGTGEGSYYYSRTRMAISGTLTVDGRPLKVIGEAWFDHQWG
                     NFSTYKEGWDWYAIQLDNDTELMIYIIHAPAGGPLIVDGSFIAADGSLTVLDRGDFTV
                     TATGSWTSPHSGAIYPSGWRIEYPAEQLTLELTPSLPDQELDTTASTDVIYWEGEVAA
                     FGTHAGRPVAGLGYVELTGYAGTDRKATPPKP"
     gene            complement(1742..3187)
                     /locus_tag="VHR64_08125"
     CDS             complement(1742..3187)
                     /locus_tag="VHR64_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302832.1"
                     /translation="MSETIRTSTRHAAAHRWHWRNFVRVLLLVTFCLSTLLSVISIWM
                     HNQIADTDRYVTTVAPLASDPDIQNAIADSVSTRLEIWLNEHVSTRDLLIDRQQVLAA
                     PLSAALVDYVDKTVREYVASDQFRQQWEQINRAVHPAVSAVLTGGRTEFVVADTGTIS
                     LDLAPVVNAVIERLNENGVTVFDQLQVDQLDTTFVIFESDELAQVQGLVDRLESLAIA
                     FPILALLTLCGFVWLTPRRRQAIILAGVSLATTMAIALLLFAVARWRLLDQFDSDSSR
                     RAAEAYLDIIARVPRQAARVLAAIGLIVAGVAAATRPESRIRRAPVAARGWLTSTWRG
                     IGTKLPWLQHGLHTATSHRRSLLIALFALFSLILITGDRVTPTPATLAVLVLAVGIAA
                     ILFMHTTEPQPVSAAAVAMTPAIVSVPLAPDGTGRSAPEREDASPPPPENQPVAPSDG
                     MRSPSITISLELPDEDVKILRRLAVLLRESD"
     gene            complement(3404..3640)
                     /locus_tag="VHR64_08130"
     CDS             complement(3404..3640)
                     /locus_tag="VHR64_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302833.1"
                     /translation="MKLFTGLSRTDYQDLFRAIGAIVDAQGLRNVRMWEHQDGIVLQG
                     QTAGSMTGDGRYQTTLLTDADLEQMLSDAYERRQ"
     gene            complement(3637..4149)
                     /locus_tag="VHR64_08135"
     CDS             complement(3637..4149)
                     /locus_tag="VHR64_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302834.1"
                     /translation="MPLLFRKSKSSAFQRARDWATSLGVIGRVLDKSGLDLRDLALSL
                     VGEEVWVCGAVWWSERVQSGWTTVTLRLDGDCLSPVGSSAMPKRSPSGHTGRAGWEAK
                     LTALGILLDRLGYVVQFPLILQVGDGFAVTIQTIGTPTVPNPGLISHEFTAAQLAAAH
                     PTAIRLGGLQ"
     gene            complement(4292..>4723)
                     /locus_tag="VHR64_08140"
     CDS             complement(4292..>4723)
                     /locus_tag="VHR64_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEX3302835.1"
                     /translation="GVVVPHRAQRADLRTALNERTGNLRLIDSVDTVERFQGDERQVI
                     IYSATESDPAYLRDTGAFLFDPRRLTVAISRAKTKLVVVASESVFDYLPTDEESFKNS
                     AIWRNLREQACTIPVWIGTVQGHRVEVRANPPLGDDRPLPM"
BASE COUNT          920 a         1460 c         1491 g          852 t
ORIGIN      
        1 aggttgcggg ggccaggctc ggatcgacgg aatgcagcgc cgccagaacg agccgaaaga
       61 actgctgtgg gcggccatcg cccgcctcga gcgtcaccca tgccgccgga ctccccgctc
      121 gcgccaacca ctgagcggcg agtgtggatt tcccggcgcc ggctggagca tggaccagtg
      181 ccacccgtgc ggaaggattg ccgccaaacc aggcatccag gcgtggccgg gcgaagtgag
      241 atgtccgcag gcgcggcgga tacagctttg tcgcaagatc cggcggcgtg ttcgcaaaga
      301 ctactggcgc cggtgaggtt ccagagttac tcattgattg gcggtcttca agccttacga
      361 gcacgtaccg caggatttac acaatgagat gctttaaccc catcgtagca cagtgatcat
      421 tacggcagtc gtgttccgag gcgggagtgc gctcggtcga attggtttgt ggccctactc
      481 gaactgaaca ctcgcctacg gcttcggcgg cgtcgccttc cggtcagtcc ctgcgtaacc
      541 tgtcaattcc acgtagccga gcccggcaac gggccgcccg gcatgggtcc cgaaggcggc
      601 gacttcacct tcccagtaga tgacgtcggt cgaggcggtc gtatccagct cctgatctgg
      661 cagcgacggc gtcaactcga gcgtgagttg ctcggccgga tattcgattc gccaacctga
      721 cggatagatt gcgccggaat gcgggctggt ccatgagccg gtagccgtga cggtgaaatc
      781 gcccctgtcg agtacggtta atgagccatc agccgcaatg aacgaaccat caacgatcag
      841 cggtccgccc gccggcgcgt ggatgatgta gatcatgagc tcggtgtcgt tgtcgagctg
      901 gatcgcgtac caatcccatc cttccttgta ggtcgagaag ttgccccatt ggtggtcgaa
      961 ccacgcctca ccgatcacct tcagaggacg gccatcgaca gtcagtgttc ccgagattgc
     1021 catgcgggtc cgcgagtaat agtacgagcc ctcgccggtg ccatagtcgt aatagccgtc
     1081 gccatcatgc aaggcgggct gtttcgtggt ctgcactgtg agatcgattc cgtactcggc
     1141 catgctggcg accaggtgat cgttgcctcc ggcgccgcgc atctgccaat cgccgacgcg
     1201 caagtcaaat ccgctggcgc tcggcggtgt ctccggcacc aggccgagcc gctgatcgta
     1261 tgagaaccgc ccgatcgcgt tgtccgtgat cgcgaaatgc gccgcatagc cgagaacgtc
     1321 tccacggtga cccttgaaga tgacgtactc gaaaccgtag cgatccccgc tgtcagtgaa
     1381 caggtggccg gtgtaatacc accactcgat tccgacgtcg tgcggcgcat cgtcccgcgg
     1441 caatacgaca gggatcgggg catcgttgcg tccagcttcc gtctcgacaa acggcgacgg
     1501 agtcccagcc tgggccgccg acgtcgcagt gttcggcatt gacccgccga cgagcacaag
     1561 tgccaacatg agaatcgcca ggaaccgctg tcgcatgacc ctgccttcac catgccatgg
     1621 ggtagctggc gcgcgtggcc gaaccggatg atcgggcacc cgcgtgcata cctggcagaa
     1681 tagaccgcgg ttatgaccgg agccaagcca tcatcgtaat cgcggctggt gaccgggccc
     1741 gctaatccga ttcacgcagg agtacggcca gccgtcgcag gatctttacg tcctcgtcgg
     1801 ggagctcgag tgagatcgtg atcgatgggg aacgcatacc gtcgcttggg gctacgggct
     1861 gattctcggg gggaggtggt gacgcgtcct ctcgttccgg cgctgagcga ccggtaccgt
     1921 caggcgcaag aggaacgctg acgatcgcgg gagtcatggc gaccgcagcc gccgagacag
     1981 gctgcggctc ggtcgtgtgc ataaacaaaa tggctgcaat gccaacggcc agcaccaaca
     2041 ccgcgagcgt cgccggagtt ggtgtcacac gatcaccggt gatcagaatc aggctgaaca
     2101 atgcgaagag cgcgatcagc agtgatctcc ggtgactcgt cgccgtgtgg agtccgtgtt
     2161 gcagccacgg caactttgtt ccgatccccc gccaagtgga cgtcagccag cctcgggcgg
     2221 cgactggagc tcgtcgaatc ctgctctccg gccgggttgc ggcggctacg ccggcaacga
     2281 tcaacccgat agcagcgagg accctggcgg cttgacgcgg cacgcgagcg atgatgtcga
     2341 ggtatgcctc cgcagccctc ctgcttgagt cggagtcgaa ttgatcaagt aaccgccatc
     2401 gcgcgaccgc gaagagcagg agtgcgatcg ccatcgttgt agccaggctg acaccagcca
     2461 ggatgatggc ttggcggcga cgtggcgtca gccagacgaa accgcacagt gtgagcaggg
     2521 caaggatcgg gaaggcgatt gccagcgatt cgagacggtc gaccaggccc tggacctggg
     2581 caagctcatc cgactcgaag atgacgaacg tggtgtcgag ctgatcgacc tggagctggt
     2641 cgaagacagt gacaccgttc tcgttgagcc gctctatgac cgcgttcacg accggcgcga
     2701 gatcgagaga gatcgtcccc gtgtcggcta caacgaattc ggttcgtccg ccggtcagga
     2761 cggccgaaac cgccgggtgt acggcgcgat tgatttgttc ccactgttgc cggaactggt
     2821 cggaggcaac gtactcccgc acggtcttgt cgacgtagtc cacgagcgcc gccgacaacg
     2881 gcgcggccag gacctgctgt cggtcgatca gcaggtctcg cgtggacacg tgctcattga
     2941 gccagatctc aagcctggtc gagaccgaat cggcaatcgc gttctgaatg tccggatcgg
     3001 aggcaagcgg cgcaactgtc gtgacgtacc gatcggtgtc cgcaatctgg ttgtgcatcc
     3061 agatcgaaat gaccgagagc agcgtcgaca ggcagaacgt gacgagcagc agcacacgaa
     3121 cgaagttgcg ccaatgccag cggtgtgcgg ccgcatgacg cgtggaggtc ctgatcgttt
     3181 cggacatcgg ttcgctgcct tcactcgggc tggggcagat cccggcagtc aactggcctt
     3241 ccgcaggggc ggcgaccagg atcaacaccc gtgaatcagg caggcgtctg ccgaatcagc
     3301 cccgtcgaag ccgattttgt ccgcactcta gagcgaaaag ggttgtatca gcatcgttcc
     3361 aatggcgcat tctgcgacgg ggaccgaacg ctgcttcgtg acgctactgg cgtcgctcgt
     3421 aggcatcgct cagcatctgc tcgaggtcag cgtcggtcag caatgtggtc tgatatcgac
     3481 cgtcgccggt catgcttccg gcggtttggc cctgcaggac gatgccatcc tggtgctccc
     3541 acatgcgcac attccgcagg ccctgagcgt cgacgatggc tccgatcgcg cgaaagagat
     3601 cctggtagtc ggtccgggaa agtccggtaa aaagtttcac tgcagtcctc cgagtcgtat
     3661 cgccgtcggg tgcgcggcag cgagttgcgc cgcggtgaat tcatgactga tcagcccggg
     3721 attaggaacg gtcggagtac cgatcgtctg tatcgtgacg gcgaacccat caccaacctg
     3781 gaggatcaac gggaactgaa caacgtatcc caaccgatcg agcaggatgc cgagggccgt
     3841 caacttcgct tcccagccgg cgcggccggt atgcccgctt ggcgaccgct tcggcatcgc
     3901 ggaagatcca accggcgaga gacagtcgcc atccagccgc agcgtcacgg tcgtccagcc
     3961 gctttggacg cgctccgacc accagaccgc accgcagacc caaacctctt ccccgaccag
     4021 ggacaatgcc agatcgcgca gatcgaggcc ggacttgtcg agcacgcggc cgataacgcc
     4081 caacgacgtc gcccagtccc gggcccgttg aaatgcactc gacttcgatt tccggaacaa
     4141 cagcggcatg ctgaatcgac ctcatgaaac caacgtttcc ggctgcgaac ggcgcggccg
     4201 ttacgccaac gctaccggcc ggcagttatg gggaacaccc ccaattcctc ccaacagagc
     4261 atccggcagt tgactgagct gttccgaccg cttacatggg gagtggacga tcgtctccaa
     4321 gcgggggatt tgcgcggacc tcgacgcggt ggccctggac ggtgccgatc cagaccggaa
     4381 tggtgcaggc ttgctcgcgg agattccgcc agatcgcact gttcttgaag ctctcctcat
     4441 cggtcgggag ataatcgaat acgctctcgg aggcgaccac gaccaatttg gtcttggccc
     4501 ggctgatcgc gaccgttagg cggcgcggat cgaacaggaa cgcgccggta tcgcgaaggt
     4561 aggccgggtc actttcggtc gcgctgtaaa tgatcacctg ccgctcatct ccctggaagc
     4621 gctcgaccgt gtcgacgcta tcgatgaggc gtagatttcc ggtgcgctcg ttgagcgcgg
     4681 tgcgcagatc ggcacgctgc gcgcggtgcg gcaccacgac acc
//
LOCUS       DATAOZ010000314         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3285080, whole genome shotgun sequence.
ACCESSION   DATAOZ010000314 DATAOZ010000000
VERSION     DATAOZ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3285080"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VHR64_08145"
     CDS             <1..656
                     /locus_tag="VHR64_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX3302836.1"
                     /translation="ELSPALGRYLEAIFYLLAEGENPRPSRIAEWLGLSKPTVTGGLR
                     RLADAGLLAPDTKSPSLTSAGRSRAERIVRTHRILERWLTDELRLDWLQADIEAGRLE
                     HAVSDDVAERIYERMNRPTTCPHGNAIPGADFHARSERRLVALRAGERSLVRRISEAT
                     EHETPTLLRFLSDHGIGLDVEVAMIDRDSGSGVVTVGVGGDRIAFAEAMAEKVWVDS"
     gene            702..1175
                     /locus_tag="VHR64_08150"
     CDS             702..1175
                     /locus_tag="VHR64_08150"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX3302837.1"
                     /translation="MTETDGNDEDHDDRINRELIELLNELRVALPGVQILFAFMLILP
                     FSQGFAKVTTAERWVYFVAFIAAALGTALLIAPSSYHRLRFRHGDKERLLIASNRMAI
                     GGMALIAIAMACVVGLITDVIFGTIPAAIAAGLVGGWFAWFWYGLPLTRRFGKDD"
BASE COUNT          297 a          578 c          593 g          270 t
ORIGIN      
        1 aagaactctc cccggccctc ggccgctacc ttgaagcgat cttctatctg ctcgccgaag
       61 gagagaaccc gcggccgagt cggatcgcgg aatggctcgg gttgtcgaag ccgaccgtca
      121 cggggggcct tcggcggctt gccgacgcgg ggctcctcgc acccgacacc aaatccccga
      181 gcttgaccag tgccggacgc agtcgcgccg agcgaatcgt tcggacgcac cgcatcctgg
      241 agcgctggct gacggatgag ctccgcctcg actggctcca ggccgacatc gaagcgggcc
      301 ggctcgagca cgcggtatcc gacgacgtcg ccgaacgcat ctacgaacgg atgaatcgtc
      361 ccaccacgtg cccccacgga aacgcgattc cgggtgccga tttccacgcg aggagcgagc
      421 gccggctcgt ggctcttcga gcgggcgagc gctcgctggt gagacgcatc tcggaggcga
      481 ccgagcacga gacgccgacg ctcctccgct ttctctcgga tcatgggatc gggctggacg
      541 tcgaggtggc gatgatcgac cgggattcgg gctccggggt cgtgaccgtt ggggtgggcg
      601 gagaccggat cgccttcgcg gaggcgatgg cggagaaggt ctgggtcgac tcgtaggcag
      661 cctcactgcc gggtaaggga accaccggaa cgggaggccc aatgaccgag acggacggga
      721 acgacgagga ccacgacgat cgaatcaacc gcgagctcat cgagctgctg aacgagctcc
      781 gcgtggcgtt gccgggcgtg cagatcctct tcgccttcat gctcatcctg ccgttcagcc
      841 agggcttcgc caaggtcacg accgccgagc ggtgggtcta cttcgtcgcg ttcatcgccg
      901 cggcgctcgg gacggcgctc ctgatcgcac cgtcctccta ccaccggctc cggtttcgcc
      961 acggagacaa agagcgcctg ctgatcgcct ccaaccggat ggcgatcggc ggcatggccc
     1021 tcatcgcgat cgccatggcg tgcgtggtgg gattgatcac cgacgtcatc ttcgggacga
     1081 tccccgcggc gatcgctgcc gggctggtcg gcggatggtt cgcctggttc tggtacgggc
     1141 ttccgctcac ccggcgattc gggaaggacg actgagtccg tccgaggcac cgcttccttc
     1201 ggggcatgcc ggtggggccg gttggaggac cggcatgccc cgaaaggtac gcacaaggtc
     1261 cgtctgcccc cgggcgtcga atgccgacca tccgatgcaa cgggaaagga gtgcccgtga
     1321 gacggacctc ggaattgccg tgctcgtcgg cgccatgctg acgtgctcgg cacgaccgga
     1381 gtccaagacg ttctgggtcg cgggcgactg cgggggagac acgctccccg gctagacctc
     1441 cgaatcggag ccccggcgtg attggacaag cgccatggcc ggaggccatg atcgctgctc
     1501 cgatcaggag gaccgcagac gagacgcttc gaggagacga ccacgatcgc ggcccctgcc
     1561 ggacgcgtct tcgactacgt gagtgacttc ccgcgccacg ccgagtggtc gggccacgag
     1621 ctccaggtca cgaagacgag cgagggcccg accgcggtcg gcagcaccta ttccaccgtc
     1681 gccaagctgt tcggcaccca gcgtgagacg agcaccatca ccgagatgac cccgggga
//
LOCUS       DATAOZ010000315         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6178100, whole genome shotgun sequence.
ACCESSION   DATAOZ010000315 DATAOZ010000000
VERSION     DATAOZ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6178100"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..233)
                     /locus_tag="VHR64_08155"
                     /pseudo
     CDS             complement(78..233)
                     /locus_tag="VHR64_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084489.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(175..1935)
                     /locus_tag="VHR64_08160"
     CDS             complement(175..1935)
                     /locus_tag="VHR64_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3302838.1"
                     /translation="MPRCQTPDTAGLRSAPTVPVEAADPRRSHTSANSGGEAAPGPLM
                     LVAAYARVSTDRQDREQTIDSQVDALRRAAEQRGWHLAADLICRDDGRTGTTLARPGL
                     DRLRDLVAEGTCAAVLVCSPDRLARNFAHQVLVIDEFRRAGCEVMFLNHAFGDSPEQQ
                     RLLQMQGVFAEYERALMLERTRRGRLFWARQGRVNWGGTPTYGYRLSPRTESLPRQLV
                     VEESEAAVVRQMYRWLVEEQLSSYAISERLRERGVPTSRQSGRGWARSSVIRVLSNST
                     YKGQGWYNRRRAVDRVQPHGKTGFKDLRPGDGHSHAFRPVAEWIAVPVPAILDADLWH
                     LAQEQLARNSEQATRNNTRHRYLLSALLICGRCGRRMIGVTDGGGRRRYLCSARYPRH
                     APGACDGRSVTAEPLEDQVWRWIAQLLSDPQLLRAHFEESRGDPAVDGADERESTRLE
                     RQLKALDREVARLIDAYQAAAIELDELKERRRQIDDHARHLRDRLGELRRQRGEREQA
                     IRLLEGLEAFCASIRQSLVDPSFEVKQKVLRLVVDRVIFDESRVTIRHIVPMTPIGLQ
                     PRSGQAEILWRGEEGDHAGG"
     gene            complement(2189..2611)
                     /locus_tag="VHR64_08165"
                     /pseudo
     CDS             complement(2189..2611)
                     /locus_tag="VHR64_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084489.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          426 a          951 c          883 g          484 t
ORIGIN      
        1 tgatcgctca aaacgggctc gggaacctac ccgctggtgg cgctttaggc aagctgcccc
       61 gagatctgac agaaccgtcg ggccgacttg aaccgcttca tcggccgttc tcgtcgcctt
      121 gttggctggt gtgagttttc cgcccggttg ttgagcccct tgtgcgggcg gtgctcaacc
      181 cccggcatga tctccttctt cgccgcgcca taggatttca gcttgtccag aacgtggttg
      241 caatccaatc ggtgtcatcg ggacgatgtg gcggatggtc actcggctct cgtcgaagat
      301 cacccggtca accaccagcc gcagaacctt ctgcttgacc tcaaaggatg gatcgacgag
      361 ggattgccgg atgcttgcgc agaatgcctc aagtccttcc aggagccgga ttgcttgctc
      421 acgttcgccg cgttgtcggc gcagttcacc gagccggtcg cgcaggtgcc gggcgtgatc
      481 gtcaatctgc cgacggcgtt ccttcagctc atccagctcg atcgcggccg cttggtaggc
      541 atcaatcagc cgcgcgactt cgcggtccag ggccttgagc tggcgctcca ggcgcgtgct
      601 ttcgcgctca tcagcgccat caacggcggg gtcgccccgg ctctcctcaa agtgcgcccg
      661 cagcagctgg ggatccgaca ggagctgggc gatccagcgc cagacctgat cctcgagcgg
      721 ctccgccgtc acgcttcggc cgtcgcaggc gccgggtgca tggcgtggat agcgggccga
      781 gcagagatag cggcgtcggc ccccgccgtc cgtgacgccg atcatgcgcc ggccgcaccg
      841 gccgcagatc agcagcgcgc tcagcagata ccgatgccgg gtgttgtttc gcgtagcctg
      901 ctcgctgttg cgggccagct gctcctgggc caggtgccag aggtcggcat caaggatggc
      961 gggcaccggc accgcgatcc attccgcgac gggtcggaaa gcgtggctgt gaccgtcgcc
     1021 cgggcgcaga tccttgaacc cggtcttgcc gtgcggctgg acccgatcga cggcccggcg
     1081 gcggttgtac cagccctgac ccttgtaggt cgagttggac aggacccgga tcacgctgct
     1141 ccgcgcccag ccgcggccgc tctgtcggct ggtcgggacg ccgcgctcgc gcaggcgctc
     1201 gctgatcgcg taggaactca gctgttcctc gaccagccag cggtacatct ggcgcaccac
     1261 cgccgcctcg ctctcctcga cgaccagttg ccggggcagg ctctcggtgc gcggcgacag
     1321 acgataaccg taggtcggcg tgccacccca gttcacccgc ccctggcgcg cccagaacag
     1381 gcgcccgcgc cgggtccgct ccagcatcag ggcgcgctcg tattcggcga acacgccctg
     1441 catctggagc agcctctgct gctcggggct gtcgccgaag gcatggttga ggaacatgac
     1501 ctcgcagccg gcccgcctga actcgtcgat caccagcacc tgatgggcga agttgcgggc
     1561 cagacgatcg ggggagcaga ccagcacggc cgcgcaggtc ccctcggcga cgaggtcgcg
     1621 cagccggtcg agccccggcc gggccagggt ggtgccggtc cggccgtcgt cgcggcagat
     1681 caggtcggcc gccaagtgcc agccacgctg ttcggcggcc cggcgcaggg catcgacctg
     1741 gctgtcgatc gtctgctccc ggtcctgccg gtccgtcgac acgcgggcat aggccgccac
     1801 cagcatcagc ggacccggtg ccgcttcgcc accgctgttt gcgctggtgt gacttctccg
     1861 cggatcggca gcttcgacgg gtaccgttgg agcagatcga aggcctgccg tatccggtgt
     1921 ctggcatctc ggcatgggat cggttctccg acaatatcga ccggccggct gtcgctctcc
     1981 gcggtgccgg catcggcgga tctgttcctg gatggcatgg gccacctccc cgcgcgtcca
     2041 tgtgcgcaca ggaacaaccg cctggcgatc agctcgatgc gcagggatca caagcgctgt
     2101 tgtccaccgg agaacctctt gccctgctcg ggcgtccgac gccacgccag aggctggatc
     2161 acctcgaagc tcaagcggct ttcgattgtg accatatcag ctccgtgatc atgacgcggg
     2221 gagcccggca ctgccgcttc agcagcttgc gcagcagccg cttggccgcc ttcttgttgc
     2281 gccggctctg gaccagcacg tccagcacga tgccgtcctg gtcgacggcg cgccagagat
     2341 aatgggtctt gccggcgatc ttgagcacaa cttcgtccat gtgccacttg tcgccgggcc
     2401 ggggcagccg ccgacggatg atgttggcga actcctgacc aaacttcagg ccccactggc
     2461 ggatcgtctc gtggctgacc tcgatgccgc ggaaggccag cctctcctcg accatccgaa
     2521 ggctcaaggg aaagcgaaag tacagccaga ccgtgtagct gatcagttcc gcaggaaagc
     2581 ggtggccggc gtagagggac gagctcgtca tgccggcctc atacccgacc cgcacccctc
     2641 agacaaccca gcccgccgcc aacttgacag tgcctgcaat acgggtgcgc gacccccacc
     2701 gagtgctcgc ccttcttcat caacggcgtg tcatcgatca ccag
//
LOCUS       DATAOZ010000316         9383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2235244, whole genome shotgun sequence.
ACCESSION   DATAOZ010000316 DATAOZ010000000
VERSION     DATAOZ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235244"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2848)
                     /locus_tag="VHR64_08170"
     CDS             complement(<1..2848)
                     /locus_tag="VHR64_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3302839.1"
                     /translation="MNAPSAASTNDATETPDSGSTLKSARSDAAEQVRIEQALDQFAV
                     TLPFDLDLFQQDAIRTLLAGDSVMVAAPTGSGKTVVAEFGVYYAFRGVGRVFYTTPIK
                     ALSNQKYRDLREVYGPEVGLLTGDVSENRDARILVMTTEILRNMLLQAPWEMDDVDAV
                     IFDEIHYLADQERGTTWEESIILCPEHVQLICLSATVTNAEEIAQWIGRTHRPIRLIT
                     HDQRPVPLALYYFIDKKLHEVIDHTGLLITDFPHTGGEARRRTNRNGAGRRNRDERND
                     PSADEPQPHEIIDALAADDMLPAIYFLFSRADCQSYAERLAQMRPNLVGRAEQRRIRD
                     IIKRYMDALRPEDRELDQVQLITRLAQVGIGFHHAGLLPILKQMVESLFGSGLMQVVF
                     ATDTLALGVNMPARTVVIGRMTKWDGRRRRPLIPNEFQQMAGRAGRRGMDAFGHVVVP
                     YSAWMPFREMLTVATGDLEPVRSSFAVRYNTVLHLWDPPHGQRVRQMLRQSLSQFQAS
                     QRIRHLEHDIIEIGGDIAGTPKGCLIGLDAGDELLEDYRRVIALHTTTEQSRRRLDKQ
                     LTELQRSTTTPWGEVNRQALRKLFRHAKPGVVLYHRTRGWGVFVARTEGEGGVGLVIF
                     GDELVHVSNYRHVVFVSEHVRAGVPEDLVADILAGNQPQEQELAEVSAQLVGAPLPDV
                     AAQMAEHEARQKALIEDDERRVQTELAQSTSILEALTEERDLHPCHPCPRRKEHRDYL
                     IRIERLDQERAALEELLNRETEAEEQRIQSVLWGIREVLHRFGYMRRGYATEKADMLA
                     GVFDTDGLILCELIDRGLLNDHAPEDLAEVFSWYSFDRDFRYSNQFVLPPHFSPIRRR
                     IEEIERALISEEREHSLFISEGHNPSFYGPARAWCFGKTMVEITETIQLSEGDLVLTF
                     NKTIDLVKQVREMLEDVKPEHGLIGPLRQAEKLLC"
     gene            2970..4088
                     /locus_tag="VHR64_08175"
     CDS             2970..4088
                     /locus_tag="VHR64_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3302840.1"
                     /translation="MRIGLNALLCSSGRNYRRTGVSRYIDELVRHLAQTEGDDELIAY
                     VSKGFEPQHWDGVQLRRAAVRAEKPPVRIAWEMANLPVASRRDRLDVFHGTVNTLPIG
                     IRGAAAVTVHDLAFLRFPDQVTAKRYQYLSRMIRSSVRRADVVLVPSQATRADVIEAF
                     NIDPDRVVVTPLGVDPRFRPASPAAISVARASYGLQNPYILTVGTLEPRKNLPALVRA
                     FATLRDDVPHDLVLAGPDGWLMDEIEATIRESGIQERVRRIGFADDAALVALYSGADA
                     VAMPSLYEGFGLPVLEAMATGAPVLTSNLSSLPEVAGDAAQLVDPTRIDAIAGGLRHL
                     LESDTLRNQLRAAGPRQAAKFTWEQTAKLTRGAYQSIA"
     gene            4088..5125
                     /gene="thiL"
                     /locus_tag="VHR64_08180"
     CDS             4088..5125
                     /gene="thiL"
                     /locus_tag="VHR64_08180"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX3302841.1"
                     /translation="MVGERVRDISEFALIERLEKALPERAWASPSLHLAIGDDSAVAE
                     VTPGEMIVVTTDTLNDGVHFRLEWTTWENLGHKAIAVNLSDLAAMGAVPHMLTVSLAL
                     TGDELVHDVEQMYVGMGKLAASHQTVVAGGDITRSNGSLSITVNAIGETRRKRLLRRD
                     EAQPNDLIWVTGTIGAAAAGFELEMLPDGDPRKRAATATGLRNALHCPAPRVKAGRVL
                     ASLGTRCAMDLSDGLAGDLYKILVASNVDAEISLPDLPVAAAVQALFRDQARDLALHG
                     GEDYELLFTAPSSFTQQIREGFELIGVRASVIGQIRPRSGDRPRLVGSESHGIRHEIE
                     PRGYDHFRPEP"
     gene            5207..5782
                     /locus_tag="VHR64_08185"
     CDS             5207..5782
                     /locus_tag="VHR64_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX3302842.1"
                     /translation="MDVLEAIRTRRSIGKVTDQRPTREQIDVMLDAATWAPNHHLTHP
                     WRFVVVAGNERIRFGEVMAQSKIDRMEREGRSTEGEAERLIAKALRAPVIIAVGVEPS
                     DQPKAIEIEEIESGAAAVQNMLLAAHALGLATMWRTGDPAYDPAVARYLGFSERGRLL
                     GFVYVGYAAVEKEPSKHVPFDEITTWRGWVD"
     gene            5793..6224
                     /gene="tsaE"
                     /locus_tag="VHR64_08190"
     CDS             5793..6224
                     /gene="tsaE"
                     /locus_tag="VHR64_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX3302843.1"
                     /translation="MAGSADETRAIGADLAGRLRAGDIVLLRGDLGSGKTTLTQGIAR
                     GLGVEGAVQSPTFTLVAEYDAPRVGSDGQLIHIDLYRLEGTADLDSVGLDEYLERQES
                     VVVIEWPDRADVATFPPHWSIRIEMTGDERRRIVIMEPTSE"
     gene            6217..6876
                     /gene="tsaB"
                     /locus_tag="VHR64_08195"
     CDS             6217..6876
                     /gene="tsaB"
                     /locus_tag="VHR64_08195"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX3302844.1"
                     /translation="MSDTRWLLALDTSTDWAGVALTDGEALAELNWTAGRKQTTHVMP
                     EVERLLGTMGLKPEHLGAVAVATGPGSFSGLRVGLAIANGLAIATEIPVLGVSTIELT
                     VHSWRDVPGLAIGVIKAGRARYGWASADEVDAPLTGTIGELVEFVRFRNASIVLGELS
                     EEDTIRVRELTGAAVPPLPGRLRHAGTLARIGWERWRSGDFDPMSIPEPVYLHRSEQH
                     P"
     gene            6953..7657
                     /gene="rimI"
                     /locus_tag="VHR64_08200"
     CDS             6953..7657
                     /gene="rimI"
                     /locus_tag="VHR64_08200"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX3302845.1"
                     /translation="MIQAQPDVALSIDHMQMSDIDEVSRIERRSFTNPWPMSAYRREL
                     RRPEHNSYYVLRAKPMQPADPHELRVPRGRFLETILPARRGDPQRDLPHLAGYVGMWQ
                     MYDETHITTIAVDPVYRGRGYGELLLVTAFADALARGSAWVSLEVRVSNDSAQALYRK
                     YGMSVYGRRAAYYTDNHEDALVMWSRALKDADYVEQIGELRDRLVLRVPEVDVPDFLI
                     PRNGEPFDTDREPPIL"
     gene            7654..8745
                     /gene="tsaD"
                     /locus_tag="VHR64_08205"
     CDS             7654..8745
                     /gene="tsaD"
                     /locus_tag="VHR64_08205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX3302846.1"
                     /translation="MNILAIETSCDETAAAVIGDGRRVRSNVVWSQLAAHQRYGGVVP
                     ELAARAHLTAIGPVVRDALSGADAGWDGIDAVAVTNRPGLSGALLVGVNAAKGIALAR
                     NLPLVGVHHIEGHIAANWLIDELQDYGEIAMPAVCLIVSGGHTDLIRMTAPGHYDSLG
                     TTLDDAAGEAFDKGARLLGLGYPGGPAIQHAAETGDPGAIPFPRAWLNDSLDFSFSGL
                     KTALLRLVEPYKVEAPESPAPIREPFAAHVPPTFREDMPVADLAAGFQEAIVDVLVAK
                     TMSAAGACEARTVILAGGVAANARLRSAMERAVAEQARPPRLISPRLTYCTDNAAMIG
                     AAAHWAIQRGDLAGYDLDVIAREPLTRKG"
     gene            8755..>9383
                     /locus_tag="VHR64_08210"
     CDS             8755..>9383
                     /locus_tag="VHR64_08210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX3302847.1"
                     /translation="MHDETDAVFETAIRLAYDAGRLLRERVGRQIEISYKGAIDLVTE
                     VDRAAEALIGKGISAAHPTHAMLGEEGTSTGVPIAEAEWVWVVDPIDGTTNFAHGYPS
                     FAVSIAVVHQGVGEIGVVFDPSRDELFAARRGGRATLNGRPIQVSTVDVMSRSLIGTG
                     FSYEQNLQVEQHEIWRHLQGRCQGIRRDGSAALGVAWVAAGRTDGFYERP"
BASE COUNT         1643 a         2896 c         3136 g         1708 t
ORIGIN      
        1 ggcagagcag cttctcggcc tgccggagtg gcccgatcag cccatgctcc ggtttgacat
       61 cctcgagcat ctcccggact tgcttgacca ggtcgatggt cttgttaaag gtcaggacca
      121 gatcgccctc cgagagctga atcgtttcgg taatttcgac catcgtcttg ccaaaacacc
      181 aggcacgggc cgggccatag aacgacgggt tgtgcccctc cgatatgaac aggctgtgct
      241 cccgctcctc cgagatcaac gctcgctcga tctcctcgat ccggcgccgg atagggctga
      301 aatgcggcgg tagcacgaac tggttgctgt agcggaaatc gcgatcaaag ctgtaccacg
      361 agaaaacctc ggccagatct tcgggcgcgt ggtcattgag caggccgcgg tcgatcagct
      421 cgcagaggat caacccgtcg gtatcgaaga cgccggccag catgtccgcc ttctcggtcg
      481 catagccccg gcgcatgtac ccgaaccggt gcaggacctc ccggatgccc cagaggaccg
      541 actggatccg ctgctcctcc gcttccgtct ctcgatttag caactcttcg agagcggcgc
      601 gctcctggtc cagccgctca attcggatca ggtagtcgcg atgctccttg cggcgtggac
      661 agggatggca ggggtgcaga tcgcgctcct cggtcagcgc ctcgaggatc gaggtcgact
      721 gggcgagctc ggtctgaact cgacgttcat cgtcctcgat cagcgccttc tgacgcgcct
      781 cgtgctcggc catctgcgcg gcgacatcag gcagcggcgc tcccacaagt tgggcgctga
      841 cctcggcaag ctcttgctcc tgcggctgat tgccggccag gatgtccgcc accagatctt
      901 cgggcacgcc ggcccggaca tgctcggaga caaagacgac gtggcggtag ttgctgacgt
      961 ggaccaattc gtcgccgaag atgacgagtc caacgccgcc ttcgccctcg gtgcgcgcca
     1021 caaagacgcc ccagcccctg gtgcgatggt aaagaacgac gcccggtttg gcatgccgaa
     1081 agagcttgcg cagcgcctgt cggttgacct caccccaggg cgtggtcgtc gaccgttgca
     1141 attcggtgag ctgcttgtcg aggcgccgcc gactttgctc ggtggtcgtg tgcagcgcga
     1201 tcacgcggcg gtagtcctcg agcagctcgt cgccggcgtc gagcccgatc aggcagccct
     1261 tgggcgtgcc ggcaatatcg ccgccgatct cgatgatgtc gtgctccagg tgccggatgc
     1321 gctggcttgc ctggaattgc gagaggctct gccgcagcat ctggcgcacg cgctgtccgt
     1381 gtggcggatc ccagagatgc aaaacggtgt tgtaacggac cgcgaacgac gagcgaaccg
     1441 gctcaagatc gccggtcgcc acggtcagca tctcgcggaa cggcatccag gctgagtagg
     1501 gaaccacgac gtggccgaat gcgtccatac cgcggcgacc ggcgcgtccc gccatctgct
     1561 ggaactcgtt cgggatcagc gggcggcggc gacgaccatc ccacttggtc atccggccga
     1621 tcacgacggt gcgggccggc atgttcacgc ccagggccag cgtgtcggtc gcaaagacga
     1681 cttgcatcag tccgctaccg aagagcgact cgaccatctg cttcagaatc gggagcaggc
     1741 cggcatggtg aaagccgatc ccgacctggg ccagtcgcgt gatcaactga acctgatcga
     1801 gctcgcgatc ctcgggtcgc agtgcgtcca tgtaccgctt gatgatgtcc cgaatccggc
     1861 gttgctcggc gcggccgacc aggttggggc gcatttgcgc gaggcgctcg gcataggact
     1921 ggcaatcggc ccggctgaac aggaagtaga tcgccggcag catgtcatcc gcggccagcg
     1981 cgtcgatgat ctcgtgtggt tgcggttcgt ctgcgctcgg gtcattgcgc tcatcccggt
     2041 tgcggcggcc cgcaccgttc cggttggtgc ggcgtcgtgc ctcaccgccg gtgtgcggga
     2101 agtccgtgat gagcaaccct gtgtggtcga taacctcgtg gagcttcttg tcgatgaagt
     2161 aatagagcgc caggggcacc gggcgctggt cgtgcgtgat caggcggatc gggcggtgcg
     2221 tgcggccgat ccactgggcg atctcctcgg cattggtgac cgttgccgag agacaaatga
     2281 gctggacgtg ctccgggcag aggatgatcg actcttccca ggtcgtgccg cgctcctgat
     2341 cggcgaggta atggatctcg tcgaagatga cggcatcgac gtcatccatc tcccacggcg
     2401 cttgcaggag catgttgcgc aggatctcgg ttgtcatgac caggatccgg gcgtcgcgat
     2461 tttcggagac atcgccggtc aggagcccga cctccgggcc gtagacctcc cgcagatccc
     2521 ggtatttctg gttcgagagc gccttgatcg gcgtggtgta gaagacgcgg ccgacgccac
     2581 ggaaggcgta gtagacgccg aattccgcaa cgacggtctt gccgcttccg gtcggcgcgg
     2641 ccaccatcac cgagtcgccg gcgagcaagg tgcggatcgc gtcctgctga aataggtcaa
     2701 gatcgaacgg caacgtgacc gcgaactgat ccagcgcctg ctcgatccgg acctgttcgg
     2761 cggcgtcact gcgcgcggac ttgagcgtcg atccgctgtc gggagtttcg gttgcgtcgt
     2821 tcgtggacgc ggcggaaggg gcgttcacat gccaaccagc gttgatgagt gtagggacga
     2881 ggcgggattt gccatccgcc tgctttgatt gcagcatagc atctgcggat tgacctaacg
     2941 acgatgccga gtacttgaga ttgccccaga tgcgcattgg attgaacgca ctgctctgct
     3001 ccagtggtcg aaactaccgc cggacgggcg ttagccgcta tatcgacgag ctggttcggc
     3061 atctcgcgca aaccgaaggc gacgacgagc tcatcgcata cgtcagcaag ggctttgaac
     3121 cccagcactg ggatggagtt cagttgcggc gagcagccgt gagggccgag aaaccgccgg
     3181 tccggatcgc ctgggaaatg gcgaaccttc cggttgccag tcgccgcgat cgattggacg
     3241 tgtttcacgg gaccgtcaac acgttgccca tcgggatccg tggcgcggcg gcggtgacgg
     3301 ttcatgacct cgcgttcctc aggtttccgg accaggttac ggcaaagcgg taccagtatt
     3361 taagtcgcat gatccgatca tcggtgcggc gcgccgacgt ggttctggtg ccgtcccagg
     3421 caacccgggc tgacgtgatc gaggcgttca acatcgaccc ggaccgcgtg gtggtaacgc
     3481 cgctcggagt cgatccccgg ttcagaccgg cttcgccggc ggctatctcg gtagcaagag
     3541 cgtcgtatgg gctacagaat ccgtatatat tgacggtagg cacacttgaa cctcgcaaga
     3601 atctgccggc gctggtgcga gcgtttgcca ctttgcgaga cgacgtgcca catgacctgg
     3661 tgctggccgg acctgacggc tggctgatgg acgagatcga agcaacgatt cgggaatcgg
     3721 ggattcagga gcgggtacgg agaatcggat ttgccgatga cgcggcgttg gttgcgctct
     3781 acagcggagc tgacgccgtc gcgatgccat cgctctacga gggtttcggg cttcccgtcc
     3841 tcgaggcaat ggcgactggc gcacccgtcc tgacctcgaa tctctcgtcg ttgccggagg
     3901 tagcaggcga cgcggcgcaa ctggtcgacc cgacccggat cgacgccatt gccggtggcc
     3961 ttcgacacct tctcgagtcc gacacgctgc ggaatcagtt gcgagcggct ggtccacggc
     4021 aggcggcgaa attcacgtgg gagcagacag ccaaactgac gcggggcgca tatcagagca
     4081 tcgcctgatg gtgggtgagc gcgtccggga tatcagcgag ttcgcgctca tcgagcgatt
     4141 ggaaaaggcg ctgcccgagc gggcctgggc atctccaagt cttcacctcg cgatcggcga
     4201 cgactccgcg gtcgccgaag tcacgccggg tgagatgatc gtcgtcacga cggataccct
     4261 gaacgacggc gtccatttcc gcctggagtg gacaacctgg gagaacctgg gacacaaggc
     4321 aattgcggtc aatctgtctg acctcgcagc gatgggtgct gtcccgcaca tgctgacggt
     4381 gtcgctggcg ctgacgggcg atgagcttgt ccacgatgtc gagcagatgt acgtcggaat
     4441 gggcaaactc gccgcctcgc accaaacggt cgttgcgggc ggtgacatca cccgttcgaa
     4501 cggctcactt tcgatcacgg tcaacgcaat cggcgaaacg cgacgtaaac gtctgcttcg
     4561 ccgggatgag gcgcagccaa acgacctgat ctgggtcacc gggacgatcg gtgccgcggc
     4621 agccggattc gagcttgaaa tgctgcccga tggggacccg aggaagcgag ccgcgactgc
     4681 cacgggactc aggaacgcgc tgcattgccc ggctccccgc gtcaaggccg gccgcgttct
     4741 cgcatcgctc gggacacgct gcgcgatgga cctgagcgat ggcctggcgg gggatctcta
     4801 caagatcctg gtcgcgagca acgtcgatgc ggaaatctcg ctgcccgact tgccagttgc
     4861 cgcagcggtt caggcactct tccgggacca ggcccgcgat ctggccttgc atggcggcga
     4921 ggactacgag ctgctcttta ccgcgccgtc atccttcacc cagcagattc gcgagggctt
     4981 cgagctgatc ggcgttcgtg ccagcgtcat cggccagatt cgccccaggt cgggcgaccg
     5041 gccgcgcctc gtcggatccg agtcacatgg tattcgccac gagatcgagc cgcgcgggta
     5101 cgaccacttt cggccggaac cctgacgctc cggactgccg ggaaacgcgc ggttcggtag
     5161 gattcgtggc ggcagattcg gccacgcggt ttcagggcag ggaacaatgg acgtacttga
     5221 ggcaatccga acgcgacgca gcatcggcaa ggtcaccgat cagcgcccga cgcgggagca
     5281 gatcgatgtg atgctggacg cggcgacgtg ggcgccgaac catcacctga cgcatccgtg
     5341 gcggttcgtg gtagttgcgg gaaatgagcg gattcgcttc ggtgaggtga tggcccaatc
     5401 caaaatcgat cgcatggagc gggaaggacg gtccacggag ggagaagccg agcggttgat
     5461 cgcgaaggcc ctgcgcgcgc cggtgattat cgcggtcggg gtcgagccct cggaccagcc
     5521 gaaagcgatc gagatcgagg agatcgaatc cggtgcggcc gctgtccaga acatgctgct
     5581 ggccgcccat gcgctgggac tggccacgat gtggcgcacc ggggatccgg cctatgaccc
     5641 ggcagtcgct cggtatctcg gtttttcgga gcggggcagg ctcctcggat tcgtctatgt
     5701 cggatacgcg gccgtggaga aggagccgag caagcacgtt ccctttgatg agatcacgac
     5761 atggcggggc tgggttgact gatcgaccgc tcatggctgg ctcagcggac gagacccgtg
     5821 cgatcggcgc cgacctggcg ggccggctgc gtgccggcga catcgtcctg ctgcggggcg
     5881 acctcggctc ggggaagacg acgctgaccc agggcatcgc acggggactc ggagtcgaag
     5941 gtgccgtcca gagcccgacc ttcaccctgg ttgccgaata cgacgcgccg cgagtcggtt
     6001 cggatggaca gcttatccac atcgatctct accgcctcga gggcacggcc gatctcgatt
     6061 cggtcggact cgacgaatat ctggagcggc aagagtcggt cgtggtgatc gagtggccgg
     6121 atcgggccga cgtcgcgact tttccgcccc actggtcgat ccgaattgag atgaccgggg
     6181 acgagcgacg ccgaatagtg atcatggagc cgaccagtga gtgacacccg ctggctgttg
     6241 gccctcgaca catcgaccga ctgggcagga gtcgcgttga ccgacggcga agcactggcc
     6301 gagctgaact ggaccgccgg gcgaaagcaa accactcacg tgatgcctga ggtggagcgg
     6361 ttgcttggca cgatgggcct gaagccggag catctcggcg ccgtcgccgt tgcaaccgga
     6421 ccagggtcgt tcagcggatt gcgggtcggc ctggcgattg ccaacgggtt ggcgatcgcg
     6481 accgaaattc cggtgcttgg cgtctcgacg attgagctga cggttcatag ctggcgcgac
     6541 gtacccggtc tggccatcgg cgtgatcaaa gccgggcgcg ctcgctatgg atgggcatcg
     6601 gccgacgaag tcgacgcgcc attgaccgga acgatcggtg agcttgtcga gttcgtgcgg
     6661 ttccgcaatg cgtcgatcgt gctcggcgag ctttcggaag aggacacgat ccgggttcgg
     6721 gagctgaccg gcgccgccgt cccgccactt ccaggtcgat tgcgtcacgc cgggacactc
     6781 gcccggattg gatgggagcg ttggcggtcg ggtgatttcg atccgatgtc gatacccgag
     6841 ccagtctatc tgcaccgctc cgagcagcat ccatgattgt atcgatgtcg ggcacgtggt
     6901 aggttcagat gtggcgggct gttccccgcc gatgaccgaa gtcggaccca tcatgattca
     6961 ggcacaacca gacgtcgccc tctcgatcga tcacatgcag atgtcggaca tcgatgaggt
     7021 gtcccggatc gagcggcgct cctttaccaa tccctggcca atgtccgcct accggcgcga
     7081 gctgcgccgg ccggagcaca actcgtacta cgtactgcga gccaagccga tgcagccggc
     7141 ggaccctcac gagctgcggg tcccgcgcgg gcgattcctc gagacgatcc ttccggcccg
     7201 ccgtggcgat ccccagcgcg atttgccgca ccttgccggg tacgtcggca tgtggcagat
     7261 gtacgacgag acgcatatca ccactatcgc cgtcgacccg gtttaccgcg gtcgcgggta
     7321 tggcgagctg ctgctggtta ccgccttcgc ggacgccctg gctcgtggct ccgcctgggt
     7381 gtcgcttgaa gtgcgggtca gcaatgactc ggcccaggct ctctaccgga aatacgggat
     7441 gagcgtatac gggcgcagag ctgcgtatta cacggacaac cacgaggatg cgctggtgat
     7501 gtggtcccgc gctctcaagg acgccgacta cgtggaacag atcggcgagt tgcgggatcg
     7561 gctcgtgctg cgggttcccg aggtcgatgt cccggatttc cttatccccc ggaacggcga
     7621 gccgttcgac accgatcgcg agccgccgat cttgtgaata tcctcgccat cgaaacttcg
     7681 tgcgatgaga cagccgccgc cgtcattggt gatggacgcc gcgtgcgctc gaatgtcgtc
     7741 tggtcgcagc ttgccgccca tcagcgctac ggcggtgttg tacctgaatt ggcggcacgg
     7801 gcgcacctga ccgcaatcgg gccggtcgtg cgcgacgcac tgagcggcgc ggatgccggc
     7861 tgggacggca tcgacgctgt cgcagtcacg aaccggcctg gattgagtgg agcactgctc
     7921 gtcggcgtca atgcggccaa ggggattgcc ctggctcgca atttgccgct ggtcggggta
     7981 caccacatcg aggggcacat cgcggccaat tggctgattg acgagctgca ggactacggc
     8041 gagatcgcga tgccggccgt ctgcctgatt gtctcaggcg gtcacaccga tctgatccgc
     8101 atgaccgcac ccggtcatta cgattcgctc ggcaccacgc ttgacgacgc ggccggcgag
     8161 gcattcgaca agggcgcgcg actccttggc ttggggtatc ccggcggccc ggccattcag
     8221 cacgcggccg aaaccggtga ccccggcgcg atcccgttcc cccgggcctg gctgaacgat
     8281 tccctcgact tcagtttttc gggcctcaag accgccctgc tgcggctggt cgagccgtat
     8341 aaggtcgagg cacccgagtc gccggcgccg atcagggagc cattcgcggc gcatgttcct
     8401 ccaacattcc gcgaggacat gccggtggca gatctggccg cagggttcca ggaggcgatc
     8461 gtcgatgtcc tcgtcgccaa gacaatgtcc gccgccggcg cgtgcgaggc ccgaacggtg
     8521 atcctcgccg gcggcgttgc ggccaacgcc cggctgcgtt cggcgatgga gcgagccgtt
     8581 gcggagcagg cacggccacc gcgcctgatc agcccgcgac tgacctattg cactgataat
     8641 gcggcgatga tcggcgcggc cgcgcactgg gcgatccagc gcggcgatct cgccggctac
     8701 gacctcgatg tgatcgcccg tgagccactg accaggaagg gataaccgtt gagcatgcac
     8761 gacgagaccg atgccgtatt cgagaccgcg atccgcctgg cctacgatgc gggccgcttg
     8821 ctgcgcgaac gtgtcggccg gcagatcgag atctcgtaca agggggcgat tgacctggtc
     8881 acagaggtcg atcgcgcggc tgaggcgctg atcggcaagg gcatctccgc ggcgcatcca
     8941 acacacgcca tgctcggcga agaggggacc agcaccggcg tgccgatcgc ggaagcagaa
     9001 tgggtctggg tggtcgaccc gatcgacggc acgaccaact ttgcgcacgg ctatccgagc
     9061 tttgctgtct cgatcgccgt cgtgcaccag ggagtcggcg agatcggcgt tgtcttcgat
     9121 ccgtcccgcg acgagttgtt cgccgcacgc cggggcggac gggcgacact caacggtcga
     9181 ccgatccaag tctcgactgt tgatgtcatg tctcgatcgt tgattggtac gggattttca
     9241 tatgagcaga atctccaggt cgaacagcat gagatctggc ggcatctgca ggggaggtgc
     9301 cagggcatcc ggcgggatgg ctcggccgcg ctcggagtgg cctgggtcgc cgccggccgg
     9361 accgacggct tttatgagcg acc
//
LOCUS       DATAOZ010000317         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5126709, whole genome shotgun sequence.
ACCESSION   DATAOZ010000317 DATAOZ010000000
VERSION     DATAOZ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5126709"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VHR64_08215"
     CDS             complement(<1..854)
                     /locus_tag="VHR64_08215"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3302848.1"
                     /translation="MTRFIGLALIPTIAAAQSTGQYRGPIIDVHLHAHTTESLAQPVP
                     NPATGQMSPKTAEDHMTRSLQIMKERNVVLGIVSGPSVAAAESWKAAAPDRIMKGIGL
                     DDPSQFMKPEELDRLFREKRLDALGEVAPQYSGYSPSDPVFDGYWAVAEQHGVPVGIH
                     TGGGPPRTPYESCCSRFRLRLNDPLLLEDLLVKYPKLRVYVMHAGGFFRENALMLMTM
                     YPQIYVDIGALSWTPVAGDMLEPFLREAKRRRLLDRVMFGSDQMRWPEAIALAIDRVN
                     GLDFLTVD"
     gene            955..>2143
                     /locus_tag="VHR64_08220"
     CDS             955..>2143
                     /locus_tag="VHR64_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3302849.1"
                     /translation="MQRRIFILVALMLGASAQALAQRPPNVVLIMMDDLGYGDLGSYG
                     ASDVRTPNIDRLAREGVRLTDAYANGAVCTPTRAALISGRYQQRVGLEWVLTVSPTDR
                     ERGLPALETSLPALLKTNGYATGLLGKWHLGSKPEFGPNAHGFDEFYGFLGGAHSYYT
                     NQAELAGVGPGAPDLFENTTPVEATGYLTDEITRRAVGFITRHADQPFFLEVAYNAVH
                     WPFQPPDRPPTEAERHAARPLRQMPDDSVAATRQDYVRMLERADQGVGEILAALDRRG
                     LTSNTLVVFTNDNGGEWLSRNAPLFHRKGTLWEGGIRVPLILRWPGELPAGKVSGQVA
                     ITMDVTASILAATGAHPPASYHPDGVNILPILRGKAPLLERRLFWRWARPDRQQRAAR
                     VGHW"
BASE COUNT          395 a          706 c          645 g          397 t
ORIGIN      
        1 tcgtcgaccg ttagaaaatc gaggccattg acgcgatcga tggccagcgc aatcgcttcg
       61 ggccatcgca tctgatcgct tccgaacata acgcgatcca gcaaccgtct tctcttcgcc
      121 tccctgagaa agggttcgag catgtccccg gcgaccgggg tccaactcag cgcaccgata
      181 tccacgtaga tctgtgggta catggtcatg agcatgagtg cattctcgcg aaagaaaccg
      241 ccggcatgca tcacatacac cctcagtttt ggatatttga ccagcaggtc ttcgaggagg
      301 agaggatcat tgagcctcag tcgaaatctt gaacagcagg actcatacgg tgtgcggggc
      361 ggaccgccgc cggtgtggat gccaactggt acaccgtgct gctccgcaac tgcccagtac
      421 ccatcgaaca ccggatccga cggagaatag cctgagtact ggggtgctac ctctccgagc
      481 gcatccaatc tcttttcgcg aaacaggcga tccagctcct cgggcttcat gaactgcgaa
      541 gggtcgtcga gcccgattcc cttcatgatt cggtctggtg ccgccgcctt ccacgactcg
      601 gcggcagcga ccgacggtcc ggagacaatg ccgagcacga cgttccgttc cttcatgatc
      661 tggagcgacc gcgtcatgtg gtcttcggcg gtcttcggtg acatctgacc ggtcgctgga
      721 ttcggcaccg gctgcgcaag actctcagtc gtgtgggcgt gcaggtgcac gtcgataatc
      781 ggcccgcggt actgcccagt gctctgagcg gccgcaatcg tcggaatcaa tgcgagcccg
      841 atgaaacgcg tcatgtctac tcctcgaata gccgccggtc ttcaccttcg cgtagctgat
      901 cactttcaca ttctcatgcg tgctgcatct tgccagcagc gcaaggagcg cgcgatgcaa
      961 cgtcgaatct tcatcctggt ggcgctgatg ctaggcgcga gcgctcaggc gctggcccag
     1021 cgcccaccga acgtcgtcct catcatgatg gacgatctgg gctacggcga tctcggcagc
     1081 tacggcgcgt cggacgtccg aacacccaac atcgatcgcc tcgcgcgcga aggcgtgcgc
     1141 ctgaccgatg cctatgccaa cggagccgtg tgcacaccca cgcgcgcggc gctcatcagc
     1201 ggccgctatc agcaacgcgt cggcctcgaa tgggtgctca cagtctcgcc gacggatcga
     1261 gagcgtggcc tgcccgctct ggaaacgtcg ttaccggcgt tgctcaagac gaacggatat
     1321 gccactgggc tcctcggcaa gtggcacctc ggctcgaagc ccgagttcgg gccgaacgcc
     1381 cacggattcg acgagttcta cgggttcctc ggcggcgccc actcgtacta caccaatcag
     1441 gctgagctcg cgggagtcgg accaggcgca ccggatctct tcgagaacac gacgccggtc
     1501 gaggccaccg gatacctgac ggatgaaatc acacgtcgcg ccgtcggttt catcacgcgc
     1561 catgccgacc agcccttctt tctcgaggtg gcctacaacg ctgtccattg gcccttccag
     1621 cctccggacc ggccccccac cgaggccgag cgacatgccg cgcggcccct gcgccagatg
     1681 cctgacgatt ccgtcgcggc tacccgccag gactacgtca ggatgctcga gcgggcggat
     1741 cagggtgtgg gagaaatcct ggcggcgctc gaccggcggg gcctaacgag caataccctg
     1801 gtggttttta cgaatgacaa cgggggtgaa tggttgtcgc gcaacgcccc gctgttccat
     1861 cgtaaaggca cgctatggga aggtggcatc cgggtaccgc tgattctgcg ctggccgggg
     1921 gaactcccgg ccgggaaggt ctccggtcag gtggccatca cgatggacgt cacggcgtcg
     1981 atccttgccg ccaccggcgc gcaccctccc gcgagctacc atccggatgg cgtcaacatc
     2041 cttccgatcc ttcgcgggaa ggcaccgctc ctcgagcgcc gcctgttctg gcgctgggcc
     2101 cggcccgatc gccagcagcg cgcggcaagg gttggacact gga
//
LOCUS       DATAOZ010000318         3358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4337237, whole genome shotgun sequence.
ACCESSION   DATAOZ010000318 DATAOZ010000000
VERSION     DATAOZ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4337237"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="VHR64_08225"
     CDS             complement(<1..1321)
                     /locus_tag="VHR64_08225"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3302850.1"
                     /translation="MFSTAGLARISARHPWKIVAIWVVLIILAVGLASGLGDALTTEG
                     DFTNKPESVRADDLLKERLRGGQDQPVTETVIVRSESATVDDPAFQQVVAQTAAGLRA
                     IPEIVATVTTYDEAVAAGAPEAAQMVSADRHTALIAVTLVGDIDTAADNANAYLDAVT
                     AQRRDGYQVLTVGDISVGERFSKLAESDLVKGEGYGLLAALIVLVIVFGALVVAGVPI
                     ALAIVSIIVAVGMTAVVGRFMDLSFFVLNMITMIGLAVGIDYALFIVSRYREERRRGH
                     AKQLAIEIAGGTATKAVVFSGGTVVLALMGMFLLPLVVFRSLGAGAILVVIAAVFATM
                     TLVPAMLSLLGDKIEWPRKRRYDDIATVAAQQAYDHETIHAGFWGKVTRIVMGRPIVS
                     VVLAGGLLVALALPYFDLNRGQAGVESLPPSDVRTAYTLLGRDFPEGR"
     gene            complement(1380..2561)
                     /locus_tag="VHR64_08230"
     CDS             complement(1380..2561)
                     /locus_tag="VHR64_08230"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3302851.1"
                     /translation="MKCTRRRFMLIPAPAALALIGLGPVSLRPAHAGSATPSPVATPT
                     TWDEVLQAGLDRGLPSVALRVEQGDQVAFEGAAGYASTEKQTPVTPTDQFRIASTTKT
                     FTSVLILQQVDDGVLTLEDTVSHWLDDPVVAKIPYVDEITVRQLLNHSSGVYDWFDEK
                     SPFWQDAYFGEGADWTKVWTPQELLAYLDGDKQDPYFAPGKGVHYSNAGYFLLGMILE
                     AATGKSYADLLHARITKPLGLTGTIYGATEPMPADVADAYHLIEGALVNVSTINLSAY
                     GAAAAIISTTQDLNRFIDALLGGKLLMPATLDEMLTFGPSDHPGFEAGLGIVRWTTPD
                     GKAIGHSGDGPGCSARMYQLEQSDLTVVLLANIGGNEDPVDATYVDAIQMALTAAAPG
                     S"
     gene            complement(2597..3049)
                     /locus_tag="VHR64_08235"
     CDS             complement(2597..3049)
                     /locus_tag="VHR64_08235"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX3302852.1"
                     /translation="MPEGNESVVRRLLEEVVNHGSTDLLTRLVAADHIGHDPLGDHYG
                     PEGLRISIAEYRAAFPDLELTVDDLVTAEDKVVHRFTLRGTHAGPFMGIPPTGRVVTT
                     SGIAIDRLADGKVIESWVNLDALNLLRQLGASPTLGHPPGDAPDEPDG"
BASE COUNT          580 a         1075 c         1118 g          585 t
ORIGIN      
        1 gccggccctc cgggaagtcg cgaccgagca gcgtataggc ggtacgcaca tcgctcggcg
       61 gcagggattc gactcccgcc tggccgcggt tcagatcgaa gtagggcaac gccaaggcga
      121 ccagcagacc cccggcgagg acgacgctaa cgatcggcct gcccatcacg atccgggtga
      181 cctttcccca gaagccagcg tggatcgtct cgtgatcgta ggcctgctgt gcggcgaccg
      241 tcgcaatatc gtcataacga cgcttgcgcg gccactcgat cttgtcgccc aggaggctca
      301 acatcgccgg gaccagcgtc atcgtcgcga agactgcagc gattacgacc aggattgctc
      361 ccgcgccgag gctgcggaaa accaccagcg ggagcaggaa catccccatc agcgccagca
      421 ccaccgtgcc gcccgagaag acgacggctt tggtggcggt gccgccggca atctcgatcg
      481 ccagctgctt ggcatgtcca cggcgccgct cctcgcgata ccgggagacg atgaagagcg
      541 cgtagtcgat cccgaccgcc aggccgatca tcgtgatcat gttcaggacg aagaaggaga
      601 ggtccatgaa ccgaccgacc accgccgtca tgccgacggc gacgatgatc gagacgatgg
      661 ccagcgcgat cggtacgccg gcaacgacca gggcgccgaa aacgatgacc aggacgatca
      721 gcgcagccag caagccgtag ccttcaccct tgaccaggtc gctctccgcg agcttggaga
      781 accgttcccc gacagagatg tcgccgaccg tcaggacttg atagccgtcc cggcgctggg
      841 cagtgacagc gtccaggtag gcattggcgt tatcagccgc ggtgtcaatg tcgccgacca
      901 gggtaactgc gatcaacgcc gtgtggcgat ctgccgagac catctgcgca gcctccggag
      961 cgcctgccgc gacggcttcg tcgtatgtcg tgacggtggc gacgatctcc ggaatcgccc
     1021 gcaagccggc ggccgtctgc gcgacgacct gctggaacgc cgggtcgtct acggtggccg
     1081 attcggatcg gacgatgacc gtctcggtca ccggctgatc ctgtccgccg cgcagccgct
     1141 ccttgagcag gtcgtcggcg cggaccgatt ccggtttatt ggtgaagtcg ccctcggtgg
     1201 tcagggcgtc gccaaggccc gaggcgaggc caacggcgag aatgatcaga acgacccata
     1261 tcgcaacgat tttccaggga tgacgggcac ttatccgtgc cagtccagca gtagaaaaca
     1321 tggctcaatg ctcctttgga atgaccggga gcggcgaacc cgctcccggt gatcggcaat
     1381 tagctgcctg gcgctgctgc tgtgagcgcc atctgaatcg catcgacgta ggtcgcgtcg
     1441 acggggtcct cgttgccacc gatgttggcg agcaacacga ccgtgaggtc tgactgctcg
     1501 agttggtaca tgcgagcaga acagcccgga ccgtccccgc tgtgaccgat ggccttgccg
     1561 tcgggtgtcg tccaccggac gatgcccaga cccgcctcga agcccggatg gtccgatggg
     1621 ccaaaagtca acatctcgtc gagcgttgcc ggcatcagca acttgccgcc gaggagcgca
     1681 tcgatgaagc ggttgaggtc ctgcgtcgtc gagatgatcg cggctgccgc cccgtacgcc
     1741 gacaggttga tggtgctgac attgaccagc gcgccctcga tcaggtggta cgcgtccgcg
     1801 acgtcggcag gcatcggctc cgtggcgccg tagatcgtgc cggtcaagcc cagcggcttg
     1861 gtgatccggg catgcaacag gtccgcgtag ctcttgccag tcgccgcttc gaggatcatg
     1921 ccgagcaaga agtagccggc gttggagtag tggacgccct tgccgggggc gaagtaggga
     1981 tcctgcttgt ctccgtccag gtaggcaagc agctcctgcg gcgtccagac cttggtccag
     2041 tccgctcctt cgccgaagta ggcgtcctgc cagaacgggc tcttttcgtc gaaccagtcg
     2101 tagacgccgc tcgaatggtt caggagttgg cgcaccgtga tctcgtcgac atacgggatc
     2161 ttcgcgacga ccggatcgtc cagccagtgg ctgacggtgt cctccagcgt caggacgccg
     2221 tcgtcgacct gctgcaggat gagaacggag gtaaaggtct tggtagtgct ggcgatgcgg
     2281 aactggtcgg tcggggtgac cggagtctgc ttctcggtgc tggcgtatcc cgccgcacct
     2341 tcgaaggcga cctggtcgcc ttgctcgacg cggagggcga cgctgggcag cccgcggtcg
     2401 agcccggcct ggagcacctc gtcccaggtg gtgggcgtcg cgaccggcga cggcgtcgcc
     2461 gatccggcgt gcgccggcct gagcgacacg gggccgagtc cgatcagtgc cagtgctgct
     2521 ggcgcgggga tgagcatgaa acgtcgtcgc gtgcacttca tgggagtggt ctccttttcc
     2581 ggctgatcgc cggcattcat ccatcaggtt cgtctggcgc gtccccgggc ggatgtccca
     2641 gcgtcgggga tgcgccgagt tgccgcagca gattcagcgc gtcgaggttg acccagctct
     2701 cgatcacctt gccgtcggcg aggcgatcga ttgcgatgcc ggaggtcgtc accacccggc
     2761 cggtcggagg gatgcccatg aacgggccgg cgtgcgtgcc ccggagggtg aagcggtgca
     2821 cgaccttgtc ctccgccgtc acgaggtcgt cgactgtcag ctcgaggtca gggaaggcgg
     2881 cgcggtactc cgcgatggag atgcgcagac cttccggccc gtagtggtcc ccgagcgggt
     2941 cgtggccgat gtggtcggcc gcgacgagcc tcgtcagcag gtcagtgctg ccgtggttga
     3001 cgacttcttc aagcagccgc cgcaccacgg actcatttcc ctcgggcatc acggcgtcct
     3061 cgactgatcg cgctgaacct cggccgcccc ggatgttccc cgaggctgcg gcgcctgccg
     3121 ctgatgtcac ggtagggagt gcagggaacc ggcacattcg ccaaatggcc taatcgcatt
     3181 gcctagatcg cggtggcacc caacgagttt cgatgttgcg ggtgatcgct atgccgcaat
     3241 atcgtccgta gtggcggggc tccgtacccg ccttgaggtg cgcaaacgga tctctcacgg
     3301 tgctcgaccg gtcatccaaa cgagtccagg gccggccggt gcatgcgaag caccactt
//
LOCUS       DATAOZ010000319         4830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5395362, whole genome shotgun sequence.
ACCESSION   DATAOZ010000319 DATAOZ010000000
VERSION     DATAOZ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5395362"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VHR64_08240"
     CDS             complement(<1..828)
                     /locus_tag="VHR64_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302853.1"
                     /translation="MKSTRAHPSLVWKSVLMIVAMFGISASGLLARPARAQGEGAYFP
                     VFVLACDAYVTMKGSASGEGYPPECRGLGDVTVTAYDTEGTRLDACTTDADGTCRLAI
                     DYNGTRIFQQDTKNVPEGYRAEENVQRVFTYTEFAEIAFHNYREDVFPQRDAPRATVR
                     VNTRICPDQYSGDSFFEDCNSGVPKTDQWIFGSATYARAGKDGNALLKGIAAGNNTEI
                     IGGQGPDTGDIFFYCSLTNDPQTRIPTTVEVTAQYDGITRDFVGKMSLDPQADVTCDW
                     "
     gene            complement(896..1756)
                     /locus_tag="VHR64_08245"
     CDS             complement(896..1756)
                     /locus_tag="VHR64_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3302854.1"
                     /translation="MPAGAVGLVAIAAVCHASWNLITKRTGGGIAFLWLFGVAATILM
                     LPIAWLEVARFNRPLERQDVIAVLGSGFIHIFYFSLLQNGYRTGDLSVVYPVARGSGP
                     LLAAIVSIAVLGDRPGALAITGGLALLAGVVGMSGLSPGQVKLDLSVTLGLLTGISIA
                     CYTLWDRRSVATLDLPPVFYYWASTAAYTAFVTPAVLRTNGPVLSRSFVQDHWRPAAA
                     VALLSAASYSLVLTAMTMAPLSYVATLRESSILVATIFGIWLLGESITRRKVIAAACI
                     VLGVVGLAVG"
     gene            1830..2030
                     /locus_tag="VHR64_08250"
     CDS             1830..2030
                     /locus_tag="VHR64_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302855.1"
                     /translation="MGKQVTPEVIRATAQMIGLELGDDEIAPVSERLQLLLDAADQFN
                     HLTENTAELDVRFNAAWEVEQV"
     gene            2027..3442
                     /locus_tag="VHR64_08255"
     CDS             2027..3442
                     /locus_tag="VHR64_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3302856.1"
                     /translation="MTKPCDLSMTEAQHRMADGSLSAVELMESVLDRMPAIPAEHHPY
                     VVVRDRSDLLAEAAEADRVRATGNVGPLHGIPIGIKDIVDVCELPTKCGSRSMEDCAP
                     AIEDAPLVRGWKAAGGIILGKTVTQEFAAGTISAPSRNPWDLDRIPGGSSGGSAAAIA
                     AGMALLTIGTDTGGSIRCPGSVCGVTGLKPTYGTVSRRGIYPLAWSLDTAGPIARTVE
                     DCAVALDVISGHDPLDPGSAPVRHGSAAAEIGQDIRGLRIGLPRTFFYDKLEPELAEI
                     FDAAAECLRELGAVLVEADWDLAAEARAVSMVINRAETSAVHDRRVRTHPELVGAEWR
                     LRVKAGLLLPATTYLNAHQARIVVRQSVANYFNQHRLDAMMMPATTGVAARSDDPYLT
                     YTDGTREHVLSGYTRMTMPTNATGQPAISVPAGFTSAGLPIGMQIVGRPFAEARICRI
                     GHAYEQAARWIDRRPALTQGV"
     gene            3444..3659
                     /locus_tag="VHR64_08260"
     CDS             3444..3659
                     /locus_tag="VHR64_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302857.1"
                     /translation="MDQPDSAGIARRGAQARANLVSALRECGELADAVETFQGDELLE
                     VLAHLDSLRFVMAESGQILQGVVRGFD"
     gene            complement(3656..4228)
                     /locus_tag="VHR64_08265"
     CDS             complement(3656..4228)
                     /locus_tag="VHR64_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3302858.1"
                     /translation="MPDARPYIEDIHLTAPDREIRTPRLRLVPWTLELIDAFIANDQA
                     AAEQALDVRFPEPFGPPPETGDVLQFFHDSIAYDASNGAFVVRMMVRTEDRMAVGNIG
                     LVAPDEAGRAMYGYSVYPEFEGNGYASEAAKAMVEFGLALDSVQTVYATIPVGHTASE
                     IVSSRAGLTLTPHQIEDEGMILNIWEKSRS"
     gene            complement(4221..>4830)
                     /locus_tag="VHR64_08270"
     CDS             complement(4221..>4830)
                     /locus_tag="VHR64_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX3302859.1"
                     /translation="QAIQTALSFVSRRDRALVPDTVLANASLAAYQQRQLWMDYAGTR
                     GEPTQRVVDLYGVTFYGGSWYAVGFCNLRHDFRTFRLDRIIHHRLLEDTFVRVPGFNA
                     QEFLWQSLSIAPGMWTAVILVDGEEQAIRARIPPELGQVEPDGQRFRWECRVSDLEWM
                     ARVLMSVDFPMQVVGPPELHETLSTMAGRITSMISRAPDAIA"
BASE COUNT          878 a         1523 c         1610 g          819 t
ORIGIN      
        1 ccagtcgcag gtcacgtcgg cttggggatc gaggctcatt ttgccgacga aatcgcgcgt
       61 gattccgtcg tattgcgccg tgacctcgac cgtggtcgga atccgcgtct gggggtcgtt
      121 ggtcagcgag cagtagaaga agatgtcgcc ggtatcggga ccctgcccgc cgatgatctc
      181 ggtgttgtta ccggccgcga tgcccttcag caacgcattg ccatccttgc cggcacgggc
      241 ataggtcgcg ctgccgaaga tccactggtc ggtcttcggc acgccgctat tgcagtcctc
      301 gaagaaagag tcgccgctgt actggtcggg acatatccgc gtgttcaccc ggacggttgc
      361 ccggggcgcg tcgcgctgcg ggaagacgtc ttcccggtag ttgtggaacg cgatctcggc
      421 gaactccgtg tacgtgaaga cccgctggac gttttcctcg gcccggtagc cttccgggac
      481 gttcttcgtg tcctgctgga agatccgcgt gccgttgtag tcgatggcga gccggcaggt
      541 gccatcggcg tctgtcgtgc aggcgtcgag gcgcgtccct tcggtgtcgt aggcagtgac
      601 cgtcacatcg ccgagtccgc ggcattcggg cgggtaacct tcgcccgagg cggagccctt
      661 cattgtgacg taggcgtcgc aggcgagcac gaagaccggg aaataggctc cctcgccttg
      721 ggcccgggcg ggacgtgcga gaagtcccga cgcgctgatt ccgaacattg cgacgatcat
      781 caggaccgac ttccagacca acgacgggtg tgcgcgcgtg ctcttcatgc tcgtattgcc
      841 tcctttgcca ccacactgac ccaataactc gcagcgtgat gcgagggttt cgcaatcatc
      901 ccaccgccag gccaacgacg cccagcacaa tgcaagccgc ggctatgacc ttgcgccgcg
      961 tgatcgattc cccgagtagc cagatgccga agatcgtcgc gaccaggatg ctcgactccc
     1021 gcagggtagc gacataggag agcggggcca tggtcatcgc ggtcagcacc agggagtatg
     1081 aggccgcact cagcagggcg accgccgcag ccggacgcca gtgatcctgc acgaacgatc
     1141 tgctcagcac tggtccattc gtgcggagaa cggccggcgt gacgaacgcg gtgtaggctg
     1201 ccgtgctggc ccagtaatag aagaccggcg gcaggtcgag cgtggcgacg gatcgccggt
     1261 cccagagcgt gtagcaggca atggagatcc cggtcagcag gccgagcgtc acagacaggt
     1321 cgagtttgac ctggcccggg ctcaacccgg acatcccgac cactccagcc aacagggcga
     1381 ggccgccggt gatggcgagc gctccgggtc gatcgccgag cacggcgatg ctgacgatcg
     1441 cagccaggag cggaccggat ccccgggcga ccgggtagac gacggagagg tcgccggtcc
     1501 ggtagccgtt ttggagcagc gaaaagtaga agatgtggat gaagccgctg ccgaggaccg
     1561 cgatcacgtc ctgtcgctcg agcgggcggt tgaaccgcgc gacttcgagc caggcgatcg
     1621 gcagcatcag gatggtcgcg gcaaccccga agagccagag gaaagcaatt cccccaccgg
     1681 tgcgcttggt gatcaggttc caactcgcgt gacagaccgc ggcgatcgca accagtccaa
     1741 ctgcgccggc cggcatcgcc cttccttcac gcgattgcaa tagactgccg tagcgggcat
     1801 cgtccgggca gacaggagag acgtcaagta tgggcaagca agtcacgccc gaagtcatca
     1861 gggcaacggc ccagatgatc gggctcgagt taggggacga cgagattgcg ccggtttcgg
     1921 agcggttgca attgctgctc gatgccgcag atcagttcaa ccacctgacc gagaacacgg
     1981 ccgagctgga cgtgcgattc aacgctgcgt gggaggtcga gcaggtatga ccaagccatg
     2041 cgacctctcg atgacggaag cccagcaccg gatggcggat ggatcgctct cggcggtcga
     2101 gctgatggag tcagtcctcg atcgcatgcc ggcaatcccg gcagaacacc acccgtacgt
     2161 ggtcgtgcgc gatcgatccg atctgctggc cgaagcggcc gaggcggacc gtgtccgggc
     2221 caccggtaat gtcggtccgc tgcacggcat cccgatcggg ataaaggaca tcgttgacgt
     2281 ctgcgaattg ccgaccaaat gcggctcgcg ttcgatggag gactgcgcgc cggcgattga
     2341 ggatgcgccg ctggtgcgcg gttggaaggc agccggtggg atcatcctcg gcaagaccgt
     2401 gacccaggag ttcgcggcgg ggacgatcag cgcgccatcg cggaacccgt gggacctcga
     2461 tcgcatcccg ggcggcagct ccgggggctc ggcggcggcg atcgcggcag ggatggcgct
     2521 gctcacgatc ggcaccgaca cgggaggcag catccgctgt cccggctcgg tctgcggcgt
     2581 gacaggtctc aaaccgacct acggcaccgt cagccggcgc ggaatctatc ccctcgcctg
     2641 gtcactcgat acggccggac cgattgcgcg gaccgtcgag gactgtgcgg tcgccctcga
     2701 tgtgatctcc ggtcacgacc cactcgatcc cggctccgca ccggtgcgac acggcagcgc
     2761 ggccgctgag atcggtcagg acatccgcgg cctgcgcatt ggtctaccgc gcaccttctt
     2821 ttatgacaag cttgagcctg agctggccga gatcttcgac gcggcagccg aatgtctgcg
     2881 tgagctcggt gcggtgctcg tcgaggctga ctgggacctg gccgctgagg cccgcgcggt
     2941 ctcgatggtc atcaaccggg ccgagacctc ggccgttcat gatcggcgag tgcggacgca
     3001 cccggagctg gtcggcgccg agtggagatt gcgggtcaag gcaggcctgc ttctgccggc
     3061 aaccacgtat ctgaacgccc atcaagcgcg catcgtagtc cggcaatcgg ttgccaacta
     3121 cttcaatcag caccggctcg atgccatgat gatgccggcg acgacaggtg tcgcggcgcg
     3181 atccgacgac ccatacctga cctataccga cggaacgcgc gagcacgtcc tgtccggtta
     3241 cacgcggatg acgatgccga ccaacgcgac cggtcagccg gcgatctcgg tcccggccgg
     3301 cttcacaagc gcggggttgc cgattggcat gcagatcgtc ggccggccgt ttgccgaggc
     3361 gcggatctgc cggatcggcc acgcctacga gcaggcggcg cggtggatcg accgccgccc
     3421 ggcgctgacg cagggagttt gagatggacc aacctgatag tgcgggaatc gcgcggcgcg
     3481 gtgcgcaagc acgggccaat ctggtctccg ccttgcgcga atgcggcgag ttggccgatg
     3541 ccgtcgagac gttccagggc gacgagctgc tcgaggtgct tgctcacctc gacagcctcc
     3601 gcttcgtgat ggcggaatcc ggccagatcc tgcaaggagt cgtccgcggt ttcgactagc
     3661 tgcgcgactt ctcccagatg ttgaggatca tcccctcatc ctcgatctgg tgcggcgtaa
     3721 gcgtcaggcc ggcgcgggag gagacgattt ccgatgcggt gtgaccgacc gggattgtgg
     3781 cgtaaacggt ctggaccgaa tccagcgcaa gtccgaactc gaccatcgcc ttcgcggcct
     3841 cggatgcgta gccgttgccc tcgaattccg ggtagacgct gtagccgtac atggcccggc
     3901 cggcttcgtc cggcgcgacc aggccgatgt tgcccaccgc catccggtcc tcggtccgca
     3961 ccatcattcg caccacgaat gcgccgttgg aggcgtcgta tgcgatcgaa tcgtgaaaga
     4021 actgcaggac atcgccggtt tccggcggcg gtccgaatgg ctccgggaat cgaacgtcca
     4081 gcgcttgctc ggcggctgcc tggtcgttcg cgatgaaggc gtcgatcaac tcaagcgtcc
     4141 agggaacgag tctcagacgc ggagttcgga tctctcgatc gggtgcggtc aaatggatgt
     4201 cctcaatgta cggtcgggcg tcaggcaatt gcgtccggcg cgcgcgaaat catactcgtg
     4261 atgcgacccg ccattgtgct cagcgtctcg tggagctcgg gcggtccaac gacctgcatc
     4321 gggaagtcga ccgacatcag gacgcgcgcc atccactcga ggtccgaaac ccggcattcc
     4381 cagcggaaac gctgaccgtc cggctcgacc tggccgagct ccggaggaat gcgggcgcgg
     4441 atcgcctgct cctcgccgtc gaccagaatc acggccgtcc acattcccgg agcgatcgac
     4501 agggactgcc agagaaactc ctgcgcgttg aaaccgggaa cgcgcacgaa ggtgtcttcc
     4561 aatagccgat gatggatgat ccgatcgagc cgaaatgtgc ggaaatcgtg gcgcaagttg
     4621 caaaagccga ctgcatacca gcttccacca taaaaggtca cgccgtagag gtccacgacg
     4681 cgctgggttg gctcgccgcg cgtgccggca taatccatcc agagctggcg ttgctgatag
     4741 gcggccaagc tggcgttggc gagcaccgtg tccgggacca acgcccgatc ccgccgcgag
     4801 acgaacgaga gcgcggtctg aatcgcctgg
//
LOCUS       DATAOZ010000320         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7761778, whole genome shotgun sequence.
ACCESSION   DATAOZ010000320 DATAOZ010000000
VERSION     DATAOZ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7761778"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..357
                     /locus_tag="VHR64_08275"
     CDS             115..357
                     /locus_tag="VHR64_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302860.1"
                     /translation="MVELSESGAAVARARDEILGVLTRSASTTFQPQDLIRQIAGNND
                     RDAYRAALMSLLASGVIERADGWTVRLSSDHAPARS"
     gene            362..>1664
                     /gene="dgt"
                     /locus_tag="VHR64_08280"
     CDS             362..>1664
                     /gene="dgt"
                     /locus_tag="VHR64_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEX3302861.1"
                     /translation="MRYRRRHDSRIKPPSIDSRSEARRDRDRIIYSIEFQRLVGVTQV
                     VSPTERFPVHNRLTHSLKVAQVGRSIAEQLLYRYRESDIGQHLDPDVVDAACLAHDLG
                     HPPFGHNTERELDSLLREPGSPPGVVDDGFEGNAQSFRVVTTTAIRYQEVEQKPGLNL
                     TAATLNALLKYPWGQRENRDRRRKWGYYQSERADFNFARTECGFPLDSERRSLEAAIM
                     NLADDISYAVHDVEDFVRAGLIPTAVFTRESNEQAWFQAKSAEIAQGLSTDDVAQVLG
                     FVNLFRYKGRHTDQAMLSEFRSESVNHFITSLRIKLTEHGPDVDIPADVRTEIDVLQL
                     LTRIYVIDSPAVQSQRYGQRRMIREVFNVFLAEATTPDSPSHQRSQLLRIFPPLWQER
                     IEVVPPDEVVVIKRLIADLIASMTEQQVCDTFEKLTGRGQAS"
BASE COUNT          321 a          528 c          494 g          321 t
ORIGIN      
        1 gtgggatcag gtcgtcaccg gcatccgccg ttaccgggcc gcgctgccct gacagaggat
       61 gtaagaacat cagttctact gctatgatga ctctggagtg gaggagaggg atcagtggtt
      121 gaattgagcg agtcaggtgc agccgtcgcg cgggcgcggg acgaaatcct tggcgttctc
      181 acgcggtccg catcaaccac gtttcagccc caggatctca ttcgccaaat tgccggcaac
      241 aacgatcgcg atgcctatcg agccgcgctg atgtcgctcc ttgcgagcgg cgttatcgag
      301 cgtgcggacg gctggaccgt tcgcctcagc agcgatcacg cgcccgcccg ctcgtagggt
      361 catgcggtat cgccgccggc acgactcgag gatcaagcct ccctcgattg attcccggtc
      421 agaggctcgc cgggatcgcg accgcatcat ctattcgatc gaattccagc ggctggtcgg
      481 cgtcacccag gttgtatcgc cgactgagcg gtttcctgtc cacaatcgcc tgacacactc
      541 actgaaggtc gcccaggtcg gtcgctcgat cgccgaacag ctcctgtatc gttaccggga
      601 gagcgatatt ggacagcacc ttgacccgga tgttgtcgac gctgcgtgcc tggcgcacga
      661 cctcggtcat cctccgtttg gccacaatac tgagcgcgag ctggactccc ttctgcgcga
      721 acccggttcg ccgccaggcg tcgttgacga tggtttcgag gggaacgcgc agagctttcg
      781 agtcgtcacc acgacggcga tccgatacca ggaagtcgag caaaagcccg gtttgaatct
      841 gaccgccgca accctcaatg cgcttctcaa atatccgtgg ggtcagcgcg agaatcggga
      901 ccgccggcgg aagtgggggt actaccagtc ggagcgcgcc gatttcaact tcgcccgaac
      961 ggagtgcggc ttccccctcg atagcgagcg tcgctcgctt gaggcggcca tcatgaatct
     1021 cgcggacgat atctcgtatg cggtccatga cgtcgaggac ttcgtgcgcg cgggcctgat
     1081 tccgacggcg gtcttcaccc gggagtcgaa cgagcaagca tggttccagg ccaaaagtgc
     1141 cgagatcgcg cagggtcttt cgacagacga cgtcgcccag gtgctcgggt tcgtcaacct
     1201 cttcagatat aagggtcggc acaccgacca ggcgatgctc tccgagttcc ggtcggaatc
     1261 agtcaatcac ttcatcacct cgctgcggat caaactgaca gagcatggcc cggacgtcga
     1321 cattccggcc gatgtccgca cggagatcga tgtgctccag ctcctcaccc gcatctacgt
     1381 catcgacagc ccggccgttc aaagccagcg ctacggccaa cgccgcatga tccgcgaggt
     1441 cttcaacgtg tttttagccg aggcgacaac gccggattcg ccatctcacc agcgatcgca
     1501 gctgttacga atcttcccgc cgctctggca agagcggatc gaggtcgttc cacctgacga
     1561 agtcgtcgtg atcaagcggc tgattgccga tctgatcgca tcgatgacgg agcaacaggt
     1621 ctgcgacacc ttcgaaaagc tgaccggccg cggccaagcc tccg
//
LOCUS       DATAOZ010000321         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7235868, whole genome shotgun sequence.
ACCESSION   DATAOZ010000321 DATAOZ010000000
VERSION     DATAOZ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7235868"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VHR64_08285"
     CDS             <1..196
                     /locus_tag="VHR64_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX3302862.1"
                     /translation="GARQIETNILDFEGDLYGQVITVQLLQRLRADEHFPTVEAMVAQ
                     LRQDEIVARTFFAELTEAID"
     gene            293..865
                     /gene="infC"
                     /locus_tag="VHR64_08290"
     CDS             293..865
                     /gene="infC"
                     /locus_tag="VHR64_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX3302863.1"
                     /translation="MARPVQTRVNERIRIREVRLIDDMGNQVGIIPTRDALEMARERG
                     LDLVEVAPNAVPPVCRLMDYGKFRYEQSRKEREARKNQHVIELKEVRIRPKIDDHDLE
                     TKGRRAAKFLDDGDKVKLTVLFRGRENAHPEIGRALLDQLADLLRSHGTIEQSPRLEG
                     KTMTMLLGPLKQKPGAQEREGGTHREEAKA"
     gene            846..1052
                     /gene="rpmI"
                     /locus_tag="VHR64_08295"
     CDS             846..1052
                     /gene="rpmI"
                     /locus_tag="VHR64_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX3302864.1"
                     /translation="MKKQKLKTHKGAKRRFKVTATGKIMRTKGMKSHFRRRKSARVKR
                     QFDRMLELDQTNEKQVKRLLPYGS"
     gene            1083..1448
                     /gene="rplT"
                     /locus_tag="VHR64_08300"
     CDS             1083..1448
                     /gene="rplT"
                     /locus_tag="VHR64_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX3302865.1"
                     /translation="MARTKRGIVAHRRHKRVLAQVKGHYGTNNRLYKRAHESLMHALA
                     YAYRDRKNRKRAFRQLWIIRINAAARINGVPYSRMMQGLRLADVAIDRKMLADLAVRD
                     PEAFAAVADVAKKSLPAQA"
     gene            1455..2216
                     /locus_tag="VHR64_08305"
     CDS             1455..2216
                     /locus_tag="VHR64_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX3302866.1"
                     /translation="MRTRFVSSEQNPDIKRIKALRDRRSVRHAERLCIVEGPRFVADA
                     ANVDQPWLLVVSESRADDDLPSSGDLLIVPDRLFAQLSDTSAPQGILGVFPFPEPGLS
                     SATPMILIADGIQDPGNLGTMIRSAAALGASRVICAAGTVDPFAPKVIRSAAAAHWHV
                     PVLPAESLQDELRDVALIVTDGEARVSIDEVDLDEPIAIAIGSEALGVSESIRSLPHV
                     AAAIPMARAVESLNAGIAASIVLYEAQRQRRFGRP"
     gene            2310..2588
                     /locus_tag="VHR64_08310"
     CDS             2310..2588
                     /locus_tag="VHR64_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX3302867.1"
                     /translation="MRKPELIAAVAKKTGMPESKATEAVNSVFGTISDVLAQGDEVAI
                     SGFGTFRVAARPAREGRNPQTGEPMKIRAGKTPRFTPGASLKRTVDVS"
     gene            complement(2585..3613)
                     /gene="plsX"
                     /locus_tag="VHR64_08315"
     CDS             complement(2585..3613)
                     /gene="plsX"
                     /locus_tag="VHR64_08315"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX3302868.1"
                     /translation="MRVAVDAMGGDFGPAVVVPSSLIALNDPDYPDLSIIFVGQTDKI
                     EPLLTTLPDSQRQRVKVIDAPDEVAMSDSAARAVRSKPKSSMALGLHLLKRGEADAFM
                     SAGHSGAVMAGALGIIGRVSGVARPALVTVLPTLRGRSMLLDLGAVTDPKPPYLVQYA
                     IMATTYATHVLGIPNPKVALLSNGEEPSKGNQLVQEVFPLLAATPGINFVGNAEGKEL
                     LKGDIEIFVTDGFTGNVALKSMEGAVSALTQVLREEVTKGIVRKALALMLKPALREVR
                     LRLDYEEIGGAPLLGVNGAVIVAHGRSHERAIANAVRVASRTASQNIPAEIARSIQAG
                     GANAEVSA"
     gene            complement(3689..>4145)
                     /locus_tag="VHR64_08320"
     CDS             complement(3689..>4145)
                     /locus_tag="VHR64_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX3302869.1"
                     /translation="FDISKLRYHRVIIMTDADVDGAHIRTLLLTFFYRHLEQLIHDGY
                     LYIAQPPLYRVKSGKEEIWTYTEEEREAAIKKLGERSEVQRYKGLGEMNPEQLWDTTM
                     DPARRTLMQVKIEDGVSVDETFDMLMGSAVPPRKKFIQTHAKEVVNLDI"
BASE COUNT          822 a         1237 c         1310 g          776 t
ORIGIN      
        1 gggcgcacgg caaatcgaga ccaacattct cgattttgag ggcgacttgt atgggcaggt
       61 gatcacggtc cagctcctgc aacgattgcg ggcagacgag cactttccga ccgttgaggc
      121 gatggttgcc caactgcgcc aggacgagat tgtcgcgcgt acctttttcg ccgagttgac
      181 cgaggcgatc gattgactga gggaggttgt gcgtgctaga ctcccttggt tgccgcccgt
      241 ttgggtggca ttttgttgct gcactcatta gatgagagcc agggggtagg ccatcgcacg
      301 gccagttcaa acacgggtca acgagcgaat tcgaatccgt gaagttcgcc tgattgacga
      361 catgggcaac caggtcggca ttattccgac ccgagacgcg ctcgaaatgg cacgggagcg
      421 cgggctcgat ctggtggaag ttgcgcccaa cgcagttccg ccggtgtgcc ggctcatgga
      481 ttacggcaag ttccgctacg agcaaagccg caaggagcgc gaagctcgca agaatcagca
      541 cgtaatcgaa ttgaaagagg tacgaattcg tccgaagatc gacgatcatg atctggagac
      601 gaagggacgt cgggccgcga agttcctcga tgacggcgac aaggtcaagc tgaccgtgct
      661 gttccggggc cgcgagaatg ctcacccgga aatcgggcga gcgctgctgg accagctggc
      721 ggatttgctc cgctcgcacg gcacgattga gcagagccca cgtcttgaag gcaaaacgat
      781 gacgatgttg ctgggtccat tgaagcaaaa gcctggcgca caggagcgag agggcggtac
      841 tcaccgtgaa gaagcaaaag cttaaaacgc acaagggcgc caagcgacga ttcaaggtca
      901 cggcaacggg caagatcatg cgcaccaagg gcatgaagag ccacttccgg cgccgcaagt
      961 cggcccgcgt caagcgccag ttcgatcgga tgctggagct ggatcagacg aacgagaagc
     1021 aggtcaagcg actgcttcca tacggcagct aacccgctcc gggatttgag gattagaaag
     1081 caatggctcg tacaaagcgc ggtatcgtag cgcaccgccg gcacaagcgg gttctggccc
     1141 aggtgaaggg ccattacgga acgaacaatc gcctgtacaa gcgggcgcat gagtcgctca
     1201 tgcatgcgct ggcgtatgcg taccgggacc gcaagaaccg caagcgggca ttccggcagc
     1261 tctggatcat ccgcatcaac gcggcggcgc gcatcaacgg cgtgccctac agccggatga
     1321 tgcagggcct gcgcctcgcc gatgtcgcga tcgaccgcaa gatgctcgct gacctggcgg
     1381 ttcgcgatcc ggaggccttt gccgccgtgg ccgatgtcgc caagaagagc cttcctgccc
     1441 aggcgtaaac ccgggtgcga acccgattcg tctcaagcga gcagaatcca gacatcaagc
     1501 gcatcaaggc gctccgcgat cgacggtccg tccgacatgc ggagcgcctc tgcattgtcg
     1561 agggaccgcg cttcgtcgcc gacgcggcta acgtcgacca gccgtggctc ctcgtggtca
     1621 gcgagtcgag agcggacgac gatctgccat cttccggcga tctcctgatc gtccctgacc
     1681 ggctatttgc ccaactctcc gatacctcgg cgccacaagg catcctcggc gtgttcccat
     1741 tcccggaacc cgggttgtcg tccgcaacac cgatgattct gattgccgac ggcatccagg
     1801 atccagggaa cctgggcacg atgatccgga gcgccgccgc actcggagca agtcgagtga
     1861 tctgcgccgc gggaacggtc gatccgttcg ctccgaaggt cattcggtct gcagctgcgg
     1921 cgcattggca tgtccctgtc ttgccagccg aatccctgca ggacgagttg cgtgacgtcg
     1981 cgctgatcgt gactgacgga gaagcgaggg tctcaattga cgaggtagat ctggacgaac
     2041 cgatcgcaat cgctatcgga agcgaggcgc tcggcgtcag cgaatcgatc agatcgttgc
     2101 cacatgttgc tgcggcgatc ccgatggcgc gcgcggtcga gtctcttaac gccgggatcg
     2161 ccgcaagcat cgtcttgtat gaagcgcaac gacaacgccg gtttggccgt ccctgataag
     2221 gttggatgcg gtgatagact gcggttctgg acattcttcc cggaaatccg ggaagtttcc
     2281 gattcgagcg acacctccag gaggtagaga tgcgcaagcc agaattgatc gctgccgtcg
     2341 cgaagaagac tggaatgccg gagagtaaag cgacggaggc tgtcaactcc gtttttggaa
     2401 cgatttccga cgtcctcgcc cagggcgacg aagtggcgat cagcggcttc ggcaccttcc
     2461 gcgttgctgc tcgtccggct cgagaaggcc gaaatccgca gaccggcgag ccgatgaaga
     2521 tccgggccgg aaagacgccc cggttcaccc cgggcgcctc gctcaagcgg acggtcgacg
     2581 tcagctaggc cgagacttcc gcgttggctc cgccggcctg aatcgagcgg gctatctcag
     2641 caggaatgtt ttgcgatgcg gttcgggacg ccacccggac cgcatttgct atcgcgcgct
     2701 cgtgtgatcg gccgtgggca acgatgactg cgccattgac ccccagcagt ggagcccctc
     2761 cgatctcctc gtagtcgagg cgaagacgca cttcgcgcag ggccggtttc agcatgaggg
     2821 ccaatgcctt ccgaacgatg cctttggtca cttcctcccg gagaacctgc gtcaaggcgc
     2881 tgacggcgcc ctccatcgac ttcagggcga cgttgcccgt aaagccgtcg gtcacgaaaa
     2941 tctcgatgtc gcctttgagc agctccttgc cctccgcatt gccgacgaag ttgatccccg
     3001 gcgtggcggc gaggagcgga aagacctcct gcacaagctg gttgcccttt gacggctctt
     3061 cgccgttcga gagcagcgca actttggggt tcgggatacc caaaacatgg gtcgcgtagg
     3121 tcgtggccat gatcgcgtat tggactaggt aagggggctt gggatcggtc acagcgccga
     3181 gatcgaggag catgctcctg ccgcgcaggg tcggaaggac cgtgaccagg gccggtcgcg
     3241 ccaccccaga aactcgtcca atgatgccca gcgcgccggc catgacggct ccgctgtgtc
     3301 ctgccgacat gaacgcatcc gcttcgccgc gcttgagcaa gtgcaggccg agggccatgc
     3361 tcgatttcgg cttgctccgg acagcgcgcg cggcggaatc ggacatggcg acttcgtcgg
     3421 gcgcgtcgat aaccttgact cgctgccgct gcgaatcggg gagcgtagtc aggagcggtt
     3481 cgatcttgtc agtctgaccg acgaagatga tcgagaggtc cggatagtct ggatcattga
     3541 gtgcgatcaa ggaagacggc acgacgacgg ccggcccgaa atctccgccc atcgcatcga
     3601 ccgcaacgcg catagctgat ctcccactca atctggcacg aaaacgcccg gctgattggc
     3661 cgggcgtttc attcacgcaa tgctggagct agatgtcgag gttcacgact tccttggcgt
     3721 gggtctggat gaacttcttg cgcggcggca cagcgctacc catcagcata tcgaaggttt
     3781 cgtcgacgct gacgccgtcc tcgatcttga cctgcatcaa cgttcgccgg gccggatcca
     3841 tcgtcgtgtc ccagagctgc tccgggttca tctcgccgag acccttatag cgctggactt
     3901 cggaccgctc gccgagcttc ttgatggccg cttcgcgctc ctcttcggtg taggtccaga
     3961 tctcttcctt gccgctcttc acgcgataca gcggtggctg cgcgatgtag aggtagccgt
     4021 catggatgag ctgctccagg tgccggtaga agaaggtcag caggagtgtc cggatgtgcg
     4081 ccccgtcgac gtcggcatcg gtcatgatga tgacccggtg gtagcgcagc ttgctgatgt
     4141 cgaac
//
LOCUS       DATAOZ010000322         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6710028, whole genome shotgun sequence.
ACCESSION   DATAOZ010000322 DATAOZ010000000
VERSION     DATAOZ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6710028"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..355
                     /locus_tag="VHR64_08325"
     CDS             146..355
                     /locus_tag="VHR64_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302870.1"
                     /translation="MFRLNDLELQVHMQRHQENVHKANRASRMTWTREIPTESTGSAA
                     GWVAGLIDRFAELVRQPARSMPANT"
     gene            417..647
                     /locus_tag="VHR64_08330"
     CDS             417..647
                     /locus_tag="VHR64_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302871.1"
                     /translation="MIRISEADYGAAMSDHNTYIREAERLGPIWYDLRKRRAEANADK
                     PHGFRAFVATQFDAISSFVSASLHVKSVAEAQ"
     gene            complement(736..2160)
                     /gene="lpdA"
                     /locus_tag="VHR64_08335"
     CDS             complement(736..2160)
                     /gene="lpdA"
                     /locus_tag="VHR64_08335"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX3302872.1"
                     /translation="MAEVAAPTHQFDLVLLGGGTGGYVAAIRAKQLGLNVAVVEELIL
                     GGCCLHRGCIPTKAFLKSADLYDDVKRAAEFGVDIAGDISFNYPNALKRSMAVVDSQY
                     KGLMYLFDKKHKIPVFMGRGKLLNKTTVGVTPKDGSAPFSIQAKNIIINTGSRPRAIK
                     GIPFDGKTVINSDHAVVLEKAPASFIVRGGGATGVEWASAYHRYGIPTTLVGNVVPQE
                     DEEIQQAMERALRRQKMEVVIGARPTANDIDITKDGVTMRLKDAKGKEKTVQAEALMV
                     GIGREPVVEDIGLEELGIKKDEGGFIIADSMLRTNVEGIYAIGDVNGQQMLAHTAQHH
                     GIIAVEHICGLNPFPLDVLNSPSCTYAEPEIGSVGLTEKQARDKGINVKVGKFPMKPN
                     AKATIEGHTDGFTKIVGDADTNDLLGVHIIGPHATEMIAEAGLARLLESTPYELSISV
                     HPHPTVSEVIGEAAHDLLGHAIHI"
     gene            2329..>2801
                     /locus_tag="VHR64_08340"
     CDS             2329..>2801
                     /locus_tag="VHR64_08340"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3302873.1"
                     /translation="MTSTRETESINRKLAVREDHWCFGCGRQNPIGLKLSFYEDGEQV
                     WAPWTPAREHQGYEGIVHGGLISTVLDEVMGWAIYVRKLWAVTGAMNVRFRKPVRVGE
                     PLTARAWVESVTGRKVDVRAQLIRDSDGQVLADATALFIRVSEAQASEWQGRYFD"
BASE COUNT          518 a          894 c          874 g          515 t
ORIGIN      
        1 cacgccgcag ggttcccgct ccgtcaaaga ccggactgcg cctccgccca atggcggatg
       61 tggttagtca gggaaagatt caggatagtc ccggttagac aacgaagcgg ttgactcgat
      121 gaatttcgac ggcgagggat aggcaatgtt tcggctcaac gatctggaac ttcaggtgca
      181 catgcagagg caccaggaaa acgtccacaa ggcaaatcgc gcgtcccgca tgacctggac
      241 gagggagatt ccgactgaat cgacgggcag cgcggcggga tgggtagcgg gtctgatcga
      301 ccggttcgcc gagctggttc gccagcccgc ccgctcgatg ccggccaaca catagcgcgc
      361 atcgcggctc cggctcagcc ggccgaaccg cgcgcaggta gcgggaagga acagccatga
      421 tcaggatttc agaagcggac tacggcgccg ccatgagtga ccacaacacc tacattcgcg
      481 aggccgagcg cctcggtccg atctggtatg acctgcgcaa gcggcgggcc gaggccaacg
      541 ccgacaagcc gcacggattc cgcgcattcg tcgccacgca gttcgacgcg atcagttcgt
      601 tcgtatcggc ctcactccac gtcaagagcg tcgccgaggc gcaataagcg ggaacccgaa
      661 accatacagg caaggaaagc gggcgaggca taagcctcgc ccgctcctgt cgtcaactca
      721 cgactcacga cggacctaga tatggatcgc gtgacccagc aggtcgtgcg ctgcctcacc
      781 gatgacctcg gagaccgtcg gatgcggatg taccgagatc gacagctcat acggcgtgct
      841 ctcgagcaac cgggccaaac cggcctcggc gatcatctcg gtcgcgtgcg ggccgatgat
      901 gtggacgccg agcaggtcgt tcgtgtcggc atcgcccaca attttggtga agccgtcggt
      961 gtgcccttcg atcgtcgcct tcgcattggg tttcatcggg aacttgccga ccttgacatt
     1021 gattcccttg tcccgcgcct gcttctcggt cagcccgacg gatccgatct cgggctctgc
     1081 gtaggtgcat gagggcgagt tgagcacgtc cagcgggaat ggattcagcc cgcagatatg
     1141 ctcgacggcg ataatgccgt ggtgctgggc ggtgtgggcc agcatctgct gtccattcac
     1201 gtcgccgatc gcgtagatgc cctcgacgtt ggtgcgcagc atcgagtcgg cgatgatgaa
     1261 cccgccctcg tctttcttga tgccgagctc ctcgagaccg atatcctcga cgacaggctc
     1321 gcgaccgatg ccgaccatca gcgcctcggc ctggaccgtc ttctccttgc ccttggcgtc
     1381 cttgaggcgc atggtcacgc cgtccttggt gatgtcaatg tcattggcgg tcgggcgggc
     1441 accgataacg acctccatct tctggcgacg gagcgcgcgc tccatcgcct gctggatctc
     1501 ctcatcctcc tgcgggacga cgttgccaac gagcgtggtc ggaatcccgt accggtggta
     1561 cgccgacgcc cactcgactc cggtcgcgcc tccaccacga acgatgaagc tcgccggcgc
     1621 cttctccagc acgacagcgt gatccgagtt gatcacggtc ttgccatcaa acgggatgcc
     1681 cttgatcgcg cgcggccggg agcccgtgtt gatgatgatg ttcttcgcct gaatcgagaa
     1741 cggcgcgctg ccatccttgg gcgtcacgcc gacggtggtc ttgttgagca gcttgccgcg
     1801 acccatgaag acggggatct tgtgcttctt gtcgaagagg tacatcagcc ccttgtactg
     1861 gctgtcgacg acggccatcg atcgcttcag cgcgttggga tagttgaacg agatgtctcc
     1921 ggcaatatcg acgccgaact cggccgcgcg cttgacgtcg tcgtagaggt cggcggactt
     1981 cagaaacgcc ttcgtcggga tgcacccacg atgcaggcag catccgccaa ggatcagttc
     2041 ctcgacgacg gccacgttca ggccgagctg cttggcgcgg atcgccgcca catagccgcc
     2101 ggtgccgcca ccgagcagga cgagatcgaa ttggtgggtg ggtgccgcaa cttccgccat
     2161 taccgggctg gactcctcgt gatcaatgca ggcgcaacgc ccgccgtcca ccgggtccgt
     2221 ggcccggtac tccgtcccgg aagtatacgg gcacgaccca cgcagcccgg catctgcgca
     2281 tccggcggta gactcaatcg gaatctacct gcctccggag gcaccatcgt gacgagcaca
     2341 cgcgaaaccg aatcgatcaa tcgaaagctg gcggtcaggg aggatcactg gtgctttggc
     2401 tgcggccgcc agaacccgat cggcctcaag ctctccttct atgaggacgg cgagcaggtc
     2461 tgggcgccct ggactcctgc gcgggagcac caggggtatg aagggatcgt tcacggcggg
     2521 ctgatttcaa ccgtgctcga cgaggtgatg ggatgggcga tctacgtccg caagctctgg
     2581 gccgtcaccg gcgccatgaa tgtccgtttc cgcaaaccgg tccgtgtcgg cgagccgctg
     2641 acagcccggg catgggtcga gtccgtaacc ggtcgcaagg tcgatgtccg tgcccagctc
     2701 atccgtgata gcgacggcca agttcttgcc gatgcaaccg ccctcttcat tcgcgtctcc
     2761 gaagcgcagg ccagcgaatg gcagggccgc tacttcgacc a
//
LOCUS       DATAOZ010000323         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5131239, whole genome shotgun sequence.
ACCESSION   DATAOZ010000323 DATAOZ010000000
VERSION     DATAOZ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5131239"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VHR64_08345"
     CDS             <1..193
                     /locus_tag="VHR64_08345"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX3302874.1"
                     /translation="SHLLEHDPVLRRSIERRNPYVDPLSFLQLELIQRLRTTEDAESL
                     IRPVLLTINGIAGALKNTG"
     gene            complement(190..1734)
                     /locus_tag="VHR64_08350"
     CDS             complement(190..1734)
                     /locus_tag="VHR64_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3302875.1"
                     /translation="MTYTARRCPNCGQYNYGKAEFCQACGEDISKESPAFIPWAQHHL
                     LPPIASAVKEESRRFARVDPDAAGSGLVWTGLVVIAAGLLIDVPTQVLLLMVLGGAGI
                     VVAGLWQLRIDYGALSRLGFWLVGVAMLALVVVAWRVLEPSTPNPVHLIQAKPTETPA
                     AQAILVGDSTVPMQRGGPAHLGVNPGPPPTGTLFRSWRFDTGGELYSSPAVTRDMLVI
                     GSKSGFLYGLNPVTGEQVWSRDLGQYIVRSTPAIQDDVVYVNNGYQTLALSLKDGATI
                     WTVDISFAGNTSPTVDNTLVYVASQSGAIQALDAQTGKQRWRVQLEGLIFGSPTVDDG
                     RVIVGNDSGTVYGLDVDNGQVDWRFKADGGVFAPLMSDDGVIYFTTNAGTTYAISATT
                     GDQVWQYDAGGSTGGSVTLNEVIVGADSGGVSAIDRESGKIIWLVPTGAQIMTGPTIV
                     GKLVVVASGQTLYGLDLATGNQVFTYATGYTIQIAPTVVNGVIYIGGRDGYLDAIVGD
                     ATKPTS"
     gene            complement(1818..2447)
                     /locus_tag="VHR64_08355"
     CDS             complement(1818..2447)
                     /locus_tag="VHR64_08355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3302876.1"
                     /translation="MSTAVHLNAEDTSNYIKHTHGNPIQRKLIDRFHQVVLQKIEELK
                     PTSFLDAGCGEGFVAGLITERVPDVQLTGFDFNPSSVELARKLNPTCNFTVASIFEIP
                     FGPQEFDVTGCFEVLEHQTDPAGALREMARVTSRALVLSVPHEPYFCLANAVRGKNLD
                     IRPRGSDPDHKQFWTRKAFGEFVTSTCPDFRIQWLGGSLPWTICVATRK"
     gene            complement(2444..>2783)
                     /locus_tag="VHR64_08360"
     CDS             complement(2444..>2783)
                     /locus_tag="VHR64_08360"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX3302877.1"
                     /translation="TLGITTVYWVIQGFVLAIMLNALDVEPKPLLVLGITSLPILIGM
                     LSPLPGGAGVRETLMGGIARIHGAAVSPTIFAAVAYRLALFASSPVLYLLFRGGLSFG
                     KQPGVEMEDQ"
BASE COUNT          502 a          869 c          900 g          512 t
ORIGIN      
        1 atctcacctg ctcgagcacg atccggtctt gcgccggtca atcgagcggc gcaacccgta
       61 cgtggaccct ctctcgttcc tgcagctcga gctgattcag cgcctgcgga ctaccgagga
      121 tgccgagtcg ctgatccggc cggttctcct gacgatcaac ggtatcgccg gggccctgaa
      181 gaacacgggt tagctggttg gcttggtcgc gtctccgaca atcgcgtcga ggtagccatc
      241 tcgcccgccg atgtagatca cgccgttgac gacggtcggc gcaatctgaa tcgtataccc
      301 ggtcgcgtac gtgaacacct ggttgcctgt cgcgaggtcg aggccgtaga gtgtctggcc
      361 gctggctacc accacgagct tgccgacgat tgttggtccg gtcatgatct gcgcgccggt
      421 cggcacgagc cagatgatct tgccgctctc gcgatcgatc gcggagacgc cgccgctatc
      481 cgcgccgacg atcacttcgt tgagcgtgac cgacccgccg gtcgaaccgc ccgcgtcgta
      541 ttgccagacc tggtcgcccg tcgtggccga gattgcgtag gttgtgccgg cgtttgtcgt
      601 gaagtagatc acgccgtcat cgctcatcaa cggcgcgaaa acaccgccat ccgccttgaa
      661 gcgccaatcc acttgcccgt tgtcgacatc cagcccatag acggtaccgc tgtcgttgcc
      721 aacgatgacc cggccatcat cgaccgtggg tgacccgaag atcagtcctt cgagctggac
      781 gcgccatcgt tgcttgccgg tttgggcgtc gagcgcctga atggcgccgc tctggctcgc
      841 gacatagacc agcgtgttgt cgacggtcgg cgaggtgttg ccggcgaacg agatgtcgac
      901 cgtccagatc gtcgcgccgt ccttgagcga gagcgcgaga gtctggtagc cgttgttcac
      961 gtagacaacg tcgtcctgaa ttgcaggtgt ggagcggacg atgtactggc cgagatcgcg
     1021 cgaccagacc tgttcacccg taacggggtt gaggccgtag aggaatccgc tcttgctgcc
     1081 gatgaccagc atgtccctgg tcacggcagg cgaggagtag agctcgccac cggtgtcgaa
     1141 gcgccaggag cggaagagcg ttccggtcgg cggagggccg ggattaacgc cgagatgggc
     1201 cgggccgccg cgctgcatcg gtaccgtgct gtcgccaacc aggatggctt gggcagccgg
     1261 cgtttcggtc ggcttcgcct gtatcagatg gaccgggtta ggcgtgctcg gctccagcac
     1321 ccgccatgcg accacgacca gtgccagcat cgcgacaccg accagccaga acccgagccg
     1381 gctcagggcc ccgtaatcga tccgcagttg ccagagtccg gcgacgacaa ttccggcgcc
     1441 acccaggacc atcaggagga gaacttgcgt gggaacgtcg atcagcaagc cggccgcgat
     1501 gaccaccaga ccggtccaga ccagaccgct gccggccgca tcgggatcga cgcgggcgaa
     1561 tctccggctc tcttccttga ccgctgaggc aatcggcgga agcaggtggt gttgcgccca
     1621 gggaatgaac gcaggcgatt ccttcgagat gtcctcaccg caggcctggc agaactcggc
     1681 cttgccgtag ttgtactggc cgcaattcgg gcagcgccgc gctgtgtatg tcacgccggt
     1741 actccgttcc gcacactcat gatacgcgcg gcggaaagca ttgaagcgct caggctagca
     1801 catggctggc ggtgcgatta cttccgggtg gcgacgcaga tcgtccatgg caacgagccg
     1861 ccgagccact ggatccggaa gtccgggcat gtcgaggtca cgaactcgcc gaatgccttg
     1921 cgggtccaga actgcttgtg gtcggggtcg ctcccgcgcg gccggatatc gaggtttttg
     1981 ccgcgcacgg cgttggccag gcagaagtac ggctcatgcg ggacgcttag gaccagtgcc
     2041 cgggaggtca cgcgggccat ctcgcgcagc gcaccggccg gatcggtctg atgctcgagc
     2101 acctcgaagc agccggtcac atcgaattcc tgtggaccga acggaatctc gaagatgctt
     2161 gccaccgtga agttgcacgt cgggttcaat ttgcgggcca gctcgaccga tgacgggttg
     2221 aaatcgaagc cggtcaactg gacgtctggc acccgctccg tgatcaggcc ggccacgaag
     2281 ccctctccgc aaccggcatc caggaacgag gttggcttca gctcttcgat cttctggagg
     2341 acgacctggt ggaagcggtc gatgagcttc cgctggatcg ggtttccgtg cgtgtgcttg
     2401 atgtaattgc tggtgtcctc ggcattcagg tgaacggcgg tgctcattga tcctccatct
     2461 cgacgcccgg ttgctttccg aacgacagcc caccgcggaa taaaaggtac aggaccggac
     2521 tcgatgcaaa cagggccagg cgataggcca ccgcggcgaa gatcgtcggg gaaaccgccg
     2581 caccgtggat gcgggcgatg ccgcccatca gcgtctcgcg gactccggcg cctccgggca
     2641 gcggactgag catgccgatc aggatcggca gcgatgtgat tcccaatacc agaaggggtt
     2701 tcggctcgac atcgagcgcg ttgagcatga tcgcaagcac gaagccctgg attacccagt
     2761 agacggtggt gattccgagt gtc
//
LOCUS       DATAOZ010000324         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5922966, whole genome shotgun sequence.
ACCESSION   DATAOZ010000324 DATAOZ010000000
VERSION     DATAOZ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5922966"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..708
                     /locus_tag="VHR64_08365"
     CDS             319..708
                     /locus_tag="VHR64_08365"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3302878.1"
                     /translation="MTGYSVDIERTTEENGYFRHVLHTAGHMQLVVMSLLPREDIGQE
                     MHPDVDQFIRIEEGHGEAILNGTSYKLSEGMAIIIPAGTEHNVINTSHDKPMRLYTIY
                     SPPNHADGTIHRTKADAEAYELAAHAR"
     gene            1106..1753
                     /locus_tag="VHR64_08370"
     CDS             1106..1753
                     /locus_tag="VHR64_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302879.1"
                     /translation="MGTSQLWMMHQVGEGPYLARWRVIAPLFVGGSLFDHETYRFSQS
                     DLAALVIAGATFPALGAIIGVAIRRHDATPSDVAVIGAMTFAGMVGFVMMATAFPIGS
                     ILLPLLAAPIAGAVVRRWPCVLGMPLAWAYGGLLSMALGLWTHGIAAEWHRSVIVGEG
                     EFLGPNRLTFGDGVRALATFAIVVILPAGLLGMIMARLTTGFVRGWGHPPDEIHA"
     gene            1898..>2661
                     /locus_tag="VHR64_08375"
     CDS             1898..>2661
                     /locus_tag="VHR64_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX3302880.1"
                     /translation="MLQHRVNQKVAVAVVYICAMLMNSLDSTVVNVALPTLSREFGVD
                     SAAIEAVIVGYLVSLAVFIPASGWLGDRFGHKRILLIALAIFVTASALCGASQTLNQL
                     ILFRVLQGAGGGLLTPVGMALLFRTFPPEERVGVARILMFATIIGPAAGPILGGFMIE
                     HFTWRWIFYINLPVGIAALIFGLLCLEDHHERQEDPFDIPGFLLAGSGFAFAMYALSE
                     GSRKGWTSPVIISTALIGLALLAIFVVVELRTERPMV"
BASE COUNT          454 a          872 c          789 g          546 t
ORIGIN      
        1 ctcgagcagg ctggctgcct catcctcgaa tccgtgccca tccccggcca cgaccgcttc
       61 gaaacccgcg accccttcgg caaccggatc gagctgatct ccggtcgcga aggcgagtag
      121 gcgcggaggc gcgaagttgg aggcgtaatg caatgtgccc gcttgcgtcc caagccgcac
      181 tgccgccctc gcgctatcaa tgaaattgca acacttgctc cagaacatct ggcgccttcg
      241 aggcgatgcg gacgccatgc tcaagctagc cgctggaata ccggcagccg accacaagtc
      301 atgcaaggag gcacgacgat gactggctac agtgtggaca tcgagcgcac gaccgaggag
      361 aacggatact tccggcacgt cctgcatacg gcaggacaca tgcagctggt cgttatgtcg
      421 ctcctgccgc gtgaggatat cggccaggag atgcacccgg acgtggatca gttcatccgg
      481 atcgaggagg gtcacggcga agcgatcctg aacggcacat cgtacaagtt gagtgagggc
      541 atggccatca tcatcccggc cggcaccgag cacaacgtga tcaacacatc gcacgacaag
      601 ccgatgcggc tgtacacgat ctactcaccg ccgaatcatg cggacggcac gatccaccgc
      661 accaaggccg acgccgaagc ctacgagctc gccgcacacg cccgctaagc tggatcacat
      721 ctgcatgtct cgggccggtc acgagctaac cggccaggcg cgaaaaaaag gtggtgactc
      781 gatgagccat gagatcgaaa cctctgctcg cgattcgccg aaaggatcaa ctgcggtacc
      841 gggacatcac ctgccgcgat ggctgcctgg cgaagtgtgt agaccccggg ccggcatcga
      901 ttgcaatctg ctcatttggc tcgcgatctg gtctctcgtt ctggtcgcgt ggatctcccg
      961 ggtctgggat gaaggctttg gtgcgcttcc gctggttgag gatcgcgtcg cggtcctctc
     1021 gctagtgctc ctggcagcca tagctggtgt gctcgtgcgg agttggtact ggccacctgt
     1081 aacagcggca tcctggattg ctgcagtggg cacgtcacaa ctatggatga tgcatcaagt
     1141 tggcgaggga ccgtatctcg ctcgatggcg cgtaatcgcc ccgctcttcg tcggcggaag
     1201 tctctttgac catgaaacgt atcgcttcag tcaatccgac ctcgcggcac tcgtgattgc
     1261 cggcgctacg ttccccgcgc ttggcgccat cattggcgtc gctattcgtc ggcatgacgc
     1321 gactccatcg gacgtagcgg tcattggtgc gatgaccttc gccggcatgg ttggattcgt
     1381 gatgatggct acggcctttc cgatcggctc gattctgctg ccgcttttgg ccgctcccat
     1441 tgctggggca gtcgtccgga ggtggccatg cgtccttgga atgccactcg cctgggccta
     1501 tgggggacta ctgtcgatgg cactcggcct ctggacgcat gggattgccg ccgagtggca
     1561 tcgctccgtg atcgtcggcg agggcgagtt cctcgggccg aatcgcctca cgttcgggga
     1621 tggcgtccgt gccctggcga cgtttgccat cgtcgtcatc ttgccggccg gactcctcgg
     1681 catgatcatg gcccggctca cgacgggatt cgtacgaggc tggggacatc cgcctgatga
     1741 aattcacgcg tagatgaccg ctctccgcgt ttatctgatg ctcgtgcgct tcacaaacct
     1801 ccaccattgg ccctgatctg tccaccgggc acagaccact gactactcct ccggtatcct
     1861 tcccgggcgc ggttcgcgcg aatgacggat agatagagtg cttcagcatc gggttaatca
     1921 gaaggtcgcg gtcgccgtcg tctacatctg cgccatgctg atgaactcgc tcgactccac
     1981 cgtcgtcaac gtcgccctcc ccaccctcag tcgcgagttc ggcgtcgact ccgccgcgat
     2041 cgaagcggtc atcgtcggct acctcgtctc gctcgccgtc ttcatccccg cctcaggctg
     2101 gctgggcgat cgcttcggac acaagcgcat cctgctgatc gcgctggcga tcttcgtaac
     2161 cgcatccgcc ctttgcggcg cctcgcagac cctcaaccag ctcatcctct tccgcgtact
     2221 gcagggcgcc ggcggcggat tgctgactcc ggtcgggatg gcgctcctct tccgcacctt
     2281 cccgcccgaa gagcgagtcg gcgtcgcccg gatcctgatg ttcgcgacga tcatcggtcc
     2341 tgcggccgga ccaatcctcg gcggcttcat gatcgagcac ttcacgtggc gctggatctt
     2401 ctacatcaac cttccggtcg gcatcgcggc cctcatcttt ggtctgctct gcctcgagga
     2461 tcatcacgag cgccaggagg acccattcga tatccccggc ttcctgcttg ccggcagcgg
     2521 cttcgccttc gccatgtacg ctctcagcga aggctcgcgc aaaggctgga cgtcgcctgt
     2581 gatcatcagc acggcactga tcggcctcgc gctgctggcg atcttcgtcg tcgtcgagct
     2641 gcgcaccgag cggccgatgg t
//
LOCUS       DATAOZ010000325         6380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1186532, whole genome shotgun sequence.
ACCESSION   DATAOZ010000325 DATAOZ010000000
VERSION     DATAOZ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186532"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2024
                     /gene="glgX"
                     /locus_tag="VHR64_08380"
     CDS             <1..2024
                     /gene="glgX"
                     /locus_tag="VHR64_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX3302881.1"
                     /translation="WDGERTQFHVWSPSADKVELCLFDRDDHSRETERVEMTNSDGTW
                     SAAVDHAPPGTPYGYRATGPYKPSAGLRFNPAKLLVDPYARSITSHLVWNPAVLSYPL
                     GKPDAKKPNSDDSAPFVLRSIVVDESFDWEGTKRPATPLEDTIIYETHVKGLTELHPD
                     VPVELRGTYAGAAHPAVIGYLRDLGITAIEFLPVHTHVDDGFLVERGLAIYWGYNTLG
                     FFSPHAAYAAASTPGAEVAEFKAMVREYHRAGIEVFLDVVYNHTAESSHQGPTLSFRG
                     LHNAEFYWLSPDDQSKYFDLTGTGNTVKVDHAYVTNFVLDSLRYWVNEMGVDGFRFDL
                     APVLGRTETDFDRHAPIFEQIEDDRSLRDIKMIAEPWDVGPGGYQVGGFPDNWSEWND
                     RYRDVVRSFWRSDDGKLNEMGFRLTGSADFYGARGFGPCASINFVAAHDGMTIWDLTA
                     YQDKRNYANGDNNQDGHDNDVNQYLGPDGPTSEPDVVAARMQRVRALLATLLVSRGVP
                     MILGGDEFGRTQLGNNNAYCQDNPISWIDWTLREENAGLVAFVRDLIALRKSEPALRI
                     ARFPDDAVEEEDPWIWFDEAGNLMTEDEWNNPKRRSFGIFLDGEGNGAQSPLVLLFNA
                     GGDELTFVLPKEIDAVDGSIELVLTTIEGERNPLVAPPGSLSAIRLKTA"
     gene            complement(2021..2623)
                     /locus_tag="VHR64_08385"
     CDS             complement(2021..2623)
                     /locus_tag="VHR64_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3302882.1"
                     /translation="MQTYSAHNGITTPMAPKAESTVHPGWLSIALDDAAVEAEDHSVE
                     ESARFAAFAQLMRTAGALDRAATDILSGLGITAGAFFALLELQTVGDSGIAPSELARR
                     LAVARRTATLYVDILVKHGWVSREAHPDDKRMILARLTGAGYDLLDGIARSYKAQLSR
                     LLDSLSLGESTRLHELLNEVPLESDTLTCGFLQSALEQAD"
     gene            complement(2832..3506)
                     /locus_tag="VHR64_08390"
     CDS             complement(2832..3506)
                     /locus_tag="VHR64_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3302883.1"
                     /translation="MTESLDRTITVVLADDHGIVRAGLRALLEMQPDMRVVGEAEDAE
                     AAVQLVLELRPTVLVTDLSMPGGGLESIREIAGQGVPTKVLVLTVHAEERYLLPVLDA
                     GGSGYVRKSSAHTDLLNAIRTVARGEAFLDPAATKTLLQGYLGRVRTGDEHGFQEILS
                     EREREVVRLTAEGHSAQQAADLLSLSVKTVETYRHRAMQKLGLTNRAELVQYALRAGL
                     LVPSER"
     gene            complement(3503..4621)
                     /locus_tag="VHR64_08395"
     CDS             complement(3503..4621)
                     /locus_tag="VHR64_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3302884.1"
                     /translation="MSSRSALTEAMRSPIEGGRRTWGMAQSATQNVSRRVPLYYRVLF
                     ANAAVVVLGAIAGTAITTGIGRNAPDHALVPLIVLFASIGIGLSLLVNVLVLRAAFRP
                     MAMLNRVAEAVQDGDRDARANVEPSSDPEMAQLARALNSTLDELAEDRNQLRDLASQV
                     ISAQEDERRRVSRELHDDTAQILFAQLLQVTALKSSADPNVQTVATKLEQSTASALEG
                     VRRLALELRPPALDDLGLAEALAELSQRFRDTTGCQTSLDVRGMRGRLSPDLELVLYR
                     VAQEALTNVAKHARAGNVWIDLERTETDVSLSVKDDGRGFDRTRFSPSDGVGLGLGLF
                     GMEERAMLLGGTVRIWSTPGAGTEVFAFIPLHRHGNQI"
     gene            4685..5176
                     /locus_tag="VHR64_08400"
     CDS             4685..5176
                     /locus_tag="VHR64_08400"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX3302885.1"
                     /translation="MASTFSFDVVSRFDFQELKNAIDQSEREIHQRYDLKDTGTEIAL
                     TEKDITINTASEYSLQAVRDTIESKMVRRGLSLKILDYQKEEQASGGRMRQTAKLREG
                     LPDEVAKKLTKQIRDEFKKVTPQIQGDLIRVQAKSKDDLQAVIESLKGQDLPVALQFV
                     NYR"
     gene            5198..>6380
                     /gene="rimO"
                     /locus_tag="VHR64_08405"
     CDS             5198..>6380
                     /gene="rimO"
                     /locus_tag="VHR64_08405"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX3302886.1"
                     /translation="MTMASPSRYHIITLGCSKNRVDSDGMDALLQQRGMSASDSPDQA
                     DVVIVNTCGFLGAARDESVGVISDVLAARPDGQKVIAAGCMPALRDYSNDIPQGVDHI
                     LTTQEWFKIGDVVSTLFDLPIAPEVAGCEGMLTTFHRHQTGPSAYVKVADGCDHNCAF
                     CTIPTIKGRQISKPPLHVLQEIVDLVAHGAKEVVLVAQDTIRYGADLGIKHGLPLLLE
                     SIAEQVPNLPWVRLLYIYPTVLTLRMVDVIAEHDALLPYLDMPLQHADRDVLRAMNRP
                     SDPDMTRRLIDHARNKLPDLTMRTTFIVGYPGETGEQFERLLAFVESEQFDHVGVFTY
                     SPEPGTVAANLGDHVPTEVAQERRGRIMEVQQKISLHKNQALVGSSVQILIEGSGELE
                     NE"
BASE COUNT         1263 a         1928 c         1985 g         1204 t
ORIGIN      
        1 gttgggacgg cgaacggacg caattccatg tctggtcgcc gagcgcggac aaggtggagc
       61 tgtgcctgtt tgatcgcgac gatcactctc gcgaaacaga acgggtcgag atgaccaact
      121 ccgacggcac atggtcggcg gcagttgacc atgcgccacc tgggacgccg tacggatacc
      181 gcgcgactgg gccgtacaag ccgtcggccg gtttgcgctt caacccggcc aaattgctcg
      241 tcgacccgta tgcccgatcc atcacgagcc accttgtctg gaacccggcg gtccttagct
      301 atccgctggg caagccggac gcaaagaagc cgaattctga tgactcggct ccgttcgtgc
      361 tccgctcgat cgtcgttgat gaatcgttcg actgggaggg cacgaagcgg ccggccactc
      421 cgctcgagga cacgatcatc tacgagaccc acgtcaaggg actcaccgag ctccatccgg
      481 acgtgccggt cgaactgcgc ggaacctacg ccggcgccgc ccatccggcg gtgatcgggt
      541 acctcaggga tctcgggatc acggcgattg agttcctgcc ggttcatacg catgtcgatg
      601 acgggttcct ggtcgagcgt gggctggcga tctattgggg gtacaacacg ctcggtttct
      661 tctcgccgca cgccgcgtat gccgcagcct caactcccgg cgccgaagtc gctgagttca
      721 aggcgatggt ccgcgaatat caccgggccg ggatcgaggt gttcctggac gttgtctaca
      781 accacacggc tgagtccagt caccagggac ctaccttgtc gtttcgtggc ctgcacaacg
      841 ccgagtttta ctggctgtcg ccggatgacc agtcaaagta cttcgacctg accgggaccg
      901 gcaacacagt caaagtcgat cacgcctacg tgacgaattt cgtgctcgac tcgctccgat
      961 actgggtcaa cgagatgggc gtcgatggtt ttcgcttcga tctggcgccg gtcctcggcc
     1021 gcaccgagac ggatttcgac cggcacgcgc cgatcttcga gcagatcgag gatgacagat
     1081 cgctccggga catcaagatg attgcggagc catgggatgt cggccccggt ggttaccagg
     1141 tcggagggtt cccggacaac tggtccgagt ggaatgaccg ataccgcgat gtcgtgcggt
     1201 cgttctggcg atccgacgac ggcaagttga atgagatggg attccgtctg accggctcgg
     1261 ctgacttcta cggtgcccgc gggttcggtc cgtgtgccag catcaacttc gtggctgccc
     1321 acgacgggat gacgatctgg gatctgacgg cctaccagga taagcgaaat tacgcgaacg
     1381 gcgacaacaa ccaggacggg cacgataacg acgtcaacca atatctaggg ccggacggtc
     1441 caacctccga accggacgtc gtcgcggccc gaatgcagcg tgtacgcgcg ttgctggcca
     1501 ccctgttggt atcccggggg gtgccgatga ttctcggcgg cgatgagttc ggccggacgc
     1561 aattgggcaa taacaacgcc tactgccagg acaacccgat cagttggatc gattggacgc
     1621 tgcgcgaaga gaacgctggt ctggttgcct tcgtgcggga cctcatcgcg ctccgaaagt
     1681 ccgagccggc gctgcggatt gcccggttcc cggatgatgc cgtcgaagag gaggatccct
     1741 ggatctggtt cgacgaggcc gggaatctga tgaccgaaga cgagtggaac aatccgaagc
     1801 gacgttcctt cggcatcttc ctcgacggcg aggggaacgg cgcccagagt ccgcttgtgt
     1861 tgctgtttaa cgcgggagga gatgagctga cgttcgtctt gccgaaagag atcgacgccg
     1921 tcgacggttc aatcgagttg gtcttgacca cgattgaggg ggagcgaaac ccactggtcg
     1981 ctccccctgg cagcttgtct gctatccggt tgaaaaccgc ttaatctgcc tgttccagcg
     2041 ccgactgcag gaaaccgcag gtgagtgtgt ccgactcgag cggaacctcg ttcaggagct
     2101 cgtgcaggcg agtcgactcg ccgagggaca gcgaatcgag gagacgggac aattgcgcct
     2161 tgtagctccg cgcgatgcca tccagcagat cgtaaccggc gccggtgagg cgggcgagga
     2221 tcatccgctt gtcgtcaggg tgcgcctctc gtgacaccca gccgtgcttg accaggatgt
     2281 cgacgtaaag ggtggccgta cggcgggcga ccgcgagtcg acgcgccaac tcggatggcg
     2341 cgattccgga atcgccgact gtctggagct cgagcagcgc gaagaatgcc ccggcggtga
     2401 tgccaagccc gctcaggata tcggtggccg cgcggtctaa cgctcccgca gtacgcatca
     2461 gttgcgcaaa tgcggcgaag cgggccgatt cttcaactga gtggtcttcg gcttcaactg
     2521 ccgcatcatc gagcgcaatt gagagccagc ctggatgcac ggtcgactcc gccttcggag
     2581 ccatgggtgt cgtaataccg ttgtgcgctg aatacgtttg catgtcgtcc ttgcgtgcct
     2641 gcccccaaca acgtctttgt gtgtcagttg aggtatcgct gccagaattc acggcgtccg
     2701 cgatggagtt cgcgttcgcc gcatgacaga atgctaccga gaatcagact tttacgttga
     2761 caggcaaatc gccacgttta cggtttgcaa tgtgctggga atccccgaca ccgtgtaggg
     2821 gattctttga ttcagcgttc cgatgggacg aggagtccgg ctcgcaacgc gtactgcacg
     2881 agctcggcac gattcgtcag gcccaatttc tgcatagccc ggtgtcgata cgtctcgacc
     2941 gtcttcactg acaacgagag cagatcagcc gcctgctggg ccgaatgtcc ctcggcggtt
     3001 agccgcacga cctcacgttc acgctcggac aggatctcct gaaacccgtg ctcatcgccg
     3061 gtgcggacgc ggcccaggta cccctgcaga agtgtcttgg ttgcggctgg gtcaagaaag
     3121 gcctcgccgc gcgcgaccgt ccgaatcgcg ttcagcagat cagtgtgcgc gctcgatttc
     3181 cgcacatatc cggaaccgcc ggcgtccagc accgggagca ggtagcgctc ctcggcgtga
     3241 acggtaagca ctagcacctt ggtcggaacc ccctgacccg cgatctctcg aatggactcg
     3301 agtccgccgc ccggcatcga gaggtcagtc acaagcacgg tcggccgcaa ctcgagcacg
     3361 agctggaccg ccgcctcggc gtcttccgcc tcaccgacga cgcgcatatc cggctgcatc
     3421 tccaggagcg cccgcaatcc agcgcgaaca atcccgtggt cgtcggccag gacaaccgtg
     3481 atggtccgat cgagtgactc cgtcatatct ggtttccatg gcggtgcaag gggatgaatg
     3541 cgaagacctc ggttccggcc ccgggagttg accagatccg gaccgttccg ccgagaagca
     3601 tcgcgcgctc ctccatgcca aacaagccga ggcccagtcc gacgccatcg ctcggcgaga
     3661 aacgggttcg atcgaagcct ctgccgtcgt ccttgacaga gagcgagaca tcggtctcag
     3721 tgcgctcgag gtcgatccag acgttgccgg cgcgcgcgtg cttggcgacg ttggtcagtg
     3781 cttcctgggc gactcgatag aggacaagtt cgagatccgg gctcaggcga ccgcgcatgc
     3841 cgcggacatc cagagacgtt tggcagccgg tggtgtcccg aaatcgctgg gacagctcgg
     3901 ccagcgcctc cgccagaccg agatcgtcga gcgccggcgg tcgcaattcg agtgccagtc
     3961 tccggacccc ctcaagtgca gaggcggtcg attgctcgag cttggtggcg acggtttgca
     4021 cgttcggatc ggccgatgac ttgagcgcgg tgacttgcaa aagctgggcg aacaggatct
     4081 gggcggtgtc gtcgtgcagc tcgcgcgaga cacgtcggcg ctcatcttcc tgggcgctga
     4141 tgacctggga agccagatcg cgaagctgat tccggtcctc ggccagttcg tcgagcgtcg
     4201 aattgagcgc ccgggccagc tgggccattt ccggatcgct ggagggctcg acgttcgcgc
     4261 gggcatcgcg atcgccatcc tgaaccgcct ccgcgacccg gttcagcatc gccatcggtc
     4321 ggaacgcggc ccgcaagacc agcacgttca caagcaggct cagcccgatc ccgatcgagg
     4381 caaagaggac gattaatggg accagggcat ggtcgggcgc gttgcgtccg atccctgtgg
     4441 tgatggctgt tccggcgatg gcgccgagga cgacgacggc ggcattcgcg aaaagcaccc
     4501 gataataaag tggaacgcgc cgggagacgt tttgcgtggc cgattgggcc atcccccagg
     4561 tgcgccgtcc accttcgatc ggtgagcgca ttgcttcagt caacgcggat cgagacgaca
     4621 tttcagtcat ttgccaaggt tgggtagtat tacccggttg caggttaagg atacggagag
     4681 tccaatggct tcaacgttct cgttcgatgt ggtttcgcgc ttcgacttcc aggaactgaa
     4741 aaacgcgatc gaccagtcag agcgcgagat ccaccagcgg tatgacctca aggataccgg
     4801 cactgagatc gctctgaccg aaaaagacat aacgatcaat accgcatctg agtatagcct
     4861 gcaggcagtg cgcgacacga tcgaatcaaa gatggtacgt cgcggattgt cgctgaagat
     4921 cctcgactat cagaaagaag agcaggcatc cggagggcgc atgcgacaga ctgccaaact
     4981 ccgcgaaggt ctgcccgacg aagtcgcaaa aaagctcacg aaacagattc gcgatgagtt
     5041 caagaaggtg acgcctcaga ttcagggcga cctgatccgg gtccaggcca agagcaagga
     5101 tgaccttcag gctgtcatcg agtcgctcaa gggccaggat cttccagtcg cgctccaatt
     5161 cgtcaactat cgctaagcgt caggattccc cgcacagatg acaatggcct ctccttcccg
     5221 gtatcacatc atcacactcg gctgctcgaa gaaccgcgtg gactccgatg gcatggacgc
     5281 gcttcttcaa cagcgcggca tgagtgcgtc cgattccccg gaccaggccg atgtcgtgat
     5341 cgtcaatacg tgcggattcc tcggcgcggc gcgtgacgag tcggtcggcg tgatctccga
     5401 tgtgctcgcc gcccggcccg acggacaaaa ggtgatcgcg gccggatgta tgccggcatt
     5461 gcgagactat tcgaacgata ttccgcaggg cgtcgaccac atcctgacaa cgcaggaatg
     5521 gttcaagatc ggcgatgtcg tcagcacgct cttcgatctc ccaatcgcgc cggaagtcgc
     5581 cggctgcgag ggaatgctga ccaccttcca ccgacatcag accgggccgt ccgcctatgt
     5641 caaggttgcg gacggatgcg accacaactg cgcgttttgc acgatcccga cgattaaggg
     5701 tcgccagatc agcaagccgc cgctgcacgt attgcaggag atcgtcgacc tggtcgcgca
     5761 cggggcgaaa gaggtcgtgc tcgttgccca ggacacgatc cggtatggcg cggatctcgg
     5821 gatcaaacat ggtctgccat tgttgctcga gtcgattgcc gaacaggtcc cgaatctgcc
     5881 atgggtccga ctcctttaca tctacccaac cgttctgacg ctccgtatgg tcgacgtgat
     5941 tgcggagcac gacgcattgc ttccctatct cgatatgccg ctccagcacg ccgatcgcga
     6001 tgtgctccgg gcgatgaacc ggccgagtga tccggacatg acgcgccggc tgatcgacca
     6061 tgcccggaac aagctgcccg acctgaccat gcggacgacc ttcatcgtcg ggtatcccgg
     6121 tgagaccggg gagcaattcg agcgattgct ggcgttcgtc gagtcggagc agttcgatca
     6181 cgtcggagtc ttcacctatt ccccagagcc tggcacggtc gcggcgaacc tgggcgacca
     6241 cgtgccgaca gaggtcgccc aagagcgacg aggtcggata atggaggtcc agcagaagat
     6301 ctcgctgcac aaaaatcagg cgttggtcgg ttcgtctgtt caaatcttga tcgaaggctc
     6361 aggcgaactt gagaacgagg
//
LOCUS       DATAOZ010000326         3378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2241287, whole genome shotgun sequence.
ACCESSION   DATAOZ010000326 DATAOZ010000000
VERSION     DATAOZ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241287"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VHR64_08410"
     CDS             complement(<1..439)
                     /locus_tag="VHR64_08410"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641524.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX3302887.1"
                     /translation="MIVVMRPTAGKEQISAVVERIESIGGLAAHLSQGEERTIIGVVG
                     SLPPTLEEMLEAFDGVEKIIRVSSKIKLANWEFHPHKTRIKILDFEIGGDEITVIAGP
                     CSVESEEQTMATARAVKAAGAKMLRGGAFKPRTSPYEFRGLGKR"
     gene            complement(436..867)
                     /gene="aroH"
                     /locus_tag="VHR64_08415"
     CDS             complement(436..867)
                     /gene="aroH"
                     /locus_tag="VHR64_08415"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX3302888.1"
                     /translation="MSDGSWACRGIRGATTAIANTAEDILEATDELMRTVIALNELEP
                     ADVASVIFTTTPDLTATFPALAARDIGWTEVPLMCAHEMAVPGSLQKCVRVMIHVNTT
                     KSAAEIRHVYLKGARELRPEWAYSDDDVAGILAKVPVEVGQ"
     gene            complement(892..1725)
                     /gene="trpA"
                     /locus_tag="VHR64_08420"
     CDS             complement(892..1725)
                     /gene="trpA"
                     /locus_tag="VHR64_08420"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX3302889.1"
                     /translation="MSTRIAVSTRIQETFDRARAENRAALMPFITAGYPSLEMSGKLL
                     DALVEGGADIIEIGVPFSDPLADGATVQATSQKSLENGTTLADCIELVRAFRERGGTI
                     PILLMGYVNPFYQYGIERLGRDAATAGVDGFLIPDLPSDESEEFQLPFRALGLDLIYF
                     LAPTSTDRRIADVAHRATGFIYCVALTGVTGARDQMAEELPEYIARIRAATDLPLSIG
                     FGISKPEHAAAVAEIADGVIVASALINHLDTLPTEDQPEGARSFVRQLADATHRTPRQ
                     V"
     gene            complement(1722..2954)
                     /gene="trpB"
                     /locus_tag="VHR64_08425"
     CDS             complement(1722..2954)
                     /gene="trpB"
                     /locus_tag="VHR64_08425"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX3302890.1"
                     /translation="MVSDIVNRSGAIDPAAVPDERGRFGRYGGQYAPETLMPALDELI
                     EAWKNARNDASFHEEIAELAKNYVGRPTPLYEAQRLSESAGGARIFLKREDLAHTGAH
                     KINNALGQALLAKRMGKHRIIAETGAGQHGVATATACALFGLECIIYMGEVDIARQAP
                     NVFRMKLLGAEVRSVSSGSKTLKDAINEAIRDWVTNVRTTFYVFGTAAGMHPYPVIVR
                     DLQSVIGREARRQMLERIGRLPNSVIACVGGGSNAIGIFHPFAGDEGVELIGVEAGGH
                     GLDSGAHASTLVAGREGILHGSRSYVLQDEWGQVIETHSISAGLDYPGVGPEHAWLKD
                     VGRARYVSITDEEALEGFHRLCRTEGIIPALESSHAIAYALKLAAERPKDEALLVNLS
                     GRGDKDLNTVAEAFGVTL"
     gene            complement(3168..>3378)
                     /locus_tag="VHR64_08430"
     CDS             complement(3168..>3378)
                     /locus_tag="VHR64_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3302891.1"
                     /translation="VFGFAVEYTALTRIRPSLYAVLISLEPAVGAILGFLILSETVGW
                     IGVAGIAAVTSASLGASRSNRGGGS"
BASE COUNT          558 a         1080 c         1150 g          590 t
ORIGIN      
        1 cacgcttgcc gagcccacgg aactcgtacg gcgaggtgcg cggtttgaag gcgccgcccc
       61 gcagcatctt tgccccggcc gccttgaccg cccgggccgt cgccatggtc tgctcctcgg
      121 actcgaccga gcagggaccg gcgatgaccg tgatctcgtc gccgccgatc tcaaagtcga
      181 ggatcttgat ccgcgtctta tgcgggtgga actcccagtt ggcgagcttg atcttgctcg
      241 agacgcgaat gatcttctcg acgccgtcga aggcttcgag catctcctca agcgtcggcg
      301 gcaagctgcc cactacgccg atgatggtcc gttcttcgcc ctgggagagg tgcgcggcga
      361 gaccgccgat tgattcgatc cgttcgacga cggcgctgat ctgctccttg ccggccgtcg
      421 gccgcatgac gacgatcatt gcccgacctc cactggcacc ttggcgagga taccggcgac
      481 atcgtcgtcg gagtaggccc actcgggtcg caattcgcgc gcgcccttca gatagacatg
      541 gcggatctcg gcggcggact tggtcgtgtt gacgtggatc atgacccgca cgcatttctg
      601 gagcgagccg ggcaccgcca tctcgtgcgc gcacatcagc ggcacttcgg tccagccgat
      661 atcgcgggcg gcgagggccg ggaaggtagc agtcaagtcc ggcgtggtcg tgaagatgac
      721 gctggccacg tcggccggct cgagttcgtt gagcgcgatg acggtgcgca tcagctcatc
      781 ggtggcctcg aggatgtcct cggccgtgtt ggcaatcgcc gtcgtggccc cgcggatccc
      841 gcggcacgcc cagcttccgt cgctcacact cacagttgct ctaacccctt gtcaaacctg
      901 gcgcggcgtt cgatgcgtcg cgtctgccaa ttgccgcaca aacgaccgcg cgccttcagg
      961 ctggtcttcg gtcggtagtg tatcgagatg gttgatcagc gcgctcgcca cgatcacgcc
     1021 atcggcgatc tcggcgaccg cggcggcgtg ctcgggtttc gagatgccga agccgatcga
     1081 gagcggcaga tcggtcgctg cccgaatccg cgcgatgtac tcgggcagct cctccgccat
     1141 ctggtcgcgc gcgccggtca cgccggtcag ggcgacgcag tagatgaagc cggtcgcgcg
     1201 gtgggcgacg tcggcgatgc ggcggtcggt gctggtcggc gcgaggaagt agatcaggtc
     1261 gagcccgagc gcgcgaaagg ggagctggaa ctcctcggat tcgtccgagg gcagatccgg
     1321 gatcaggaag ccatcgaccc ctgccgtggc cgcgtcccga cccagccgct cgatgccgta
     1381 ctggtagaag ggattgacat atcccatcag caggatcggg atcgtgccgc cgcgctcgcg
     1441 gaaggcccgg acaagctcga tgcagtcggc cagcgtcgtg ccgttctcga gcgacttttg
     1501 gctggtggcc tgaaccgtcg cgccgtcggc caacggatcg gagaagggga cgccgatctc
     1561 gatgatgtcg gctccgccct cgaccagcgc gtcgagcagc ttgccgctca tctcgagcga
     1621 tgggtagccg gccgtgatga agggcatcag cgcggcccga ttctcggccc gggcccggtc
     1681 gaatgtctcc tggatgcggg tcgaaacggc gatacgtgtg ctcacagtgt caccccgaaa
     1741 gcttcggcaa ccgtattcag gtccttgtcg ccacgaccgg agaggttgac cagcagcgcc
     1801 tcatcctttg gtcgctccgc cgccagcttg agcgcgtagg cgatcgcgtg gctcgactcg
     1861 agcgccggga tgatcccttc ggtccggcag aggcggtgga agccttcgag tgcttcctcg
     1921 tccgtgatcg agacgtatcg cgcccggccg acatccttga gccaggcatg ctccgggccg
     1981 acgccggggt aatcgagacc ggccgagatc gagtgcgtct cgatgacttg accccactca
     2041 tcctgcagga cgtaggagcg ggagccgtgg aggatgcctt ctcgcccggc cacaagcgtg
     2101 gaggcgtgcg ctccggaatc gagcccatgc ccgccggcct cgacgccgat caactcgacg
     2161 ccctcatcgc cggcgaaggg atggaagata ccgatcgcgt tcgagccacc gccgacgcag
     2221 gcgatcaccg agttcgggag ccgcccgatc cgctcgagca tctggcggcg cgcctcgcgg
     2281 ccgatcaccg attgcagatc gcgaacgatg accgggtacg ggtgcatgcc ggcggccgtg
     2341 ccgaaaacgt agaaggtggt gcggacgttg gtgacccagt cgcggatcgc ttcgttgatc
     2401 gcgtccttga gcgtcttcga gccggatgag accgagcgca cctcggcgcc gagcagcttc
     2461 atccggaaga cgttgggcgc ctgccgggcg atgtcgacct cgcccatata aatgatgcat
     2521 tcgagaccga agagggcgca ggccgtggcg gtcgcgacgc cgtgctggcc ggcgccggtt
     2581 tcggcgatga tccggtgctt gcccatccgc ttggcgagca gggcctggcc gagcgcgttg
     2641 ttgatcttat gcgcgccggt gtgggccagg tcttcgcgct tgagaaagat gcgcgcgccg
     2701 ccggccgatt cgctcagccg ctgcgcctcg tagagcggag ttgggcggcc gacgtagttc
     2761 ttggccagct ccgcgatctc ctcgtgaaag gaggcgtcgt tgcgggcgtt cttccaggcc
     2821 tcgatcagct cgtcgagggc gggcatcagc gtctcgggcg catattgccc tccgtaccgg
     2881 ccgaacctgc cgcgttcatc gggcacggcg gcggggtcga tagccccaga gcgattgaca
     2941 atgtccgaaa ccatgaccga ctccacaggt acgaaacgac acgcgggccg gagcctgcac
     3001 catgccccag ccacgtcgtt ggaggtgcgc ttgattcgct tggggaatga taccgctgat
     3061 cgggttgagg gttgagggag agtcgttgga cgttggtaga tggaacggaa cggatggcca
     3121 gtacgctcat tccaacgacg aacgaccaac gaccaacgaa cttctaccta cgaccctcca
     3181 cctctgttgc tccggctggc gccgagggag gcggatgtga cggcggcgat gccggccacg
     3241 ccgatccagc cgacggtctc gctgaggatc aggaagccga ggatcgcgcc gacggctggt
     3301 tcgagcgaga tcaggaccgc gtagagcgag gggcgaatcc gggtaagggc ggtgtactcg
     3361 accgcaaagc cgaaaacg
//
LOCUS       DATAOZ010000327         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4609703, whole genome shotgun sequence.
ACCESSION   DATAOZ010000327 DATAOZ010000000
VERSION     DATAOZ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4609703"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..679)
                     /gene="hisB"
                     /locus_tag="VHR64_08435"
     CDS             complement(74..679)
                     /gene="hisB"
                     /locus_tag="VHR64_08435"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX3302892.1"
                     /translation="MTAMPIRTRTIERKTRETEITVTVELDGAGSVDVETGVHFLDHM
                     LDALGRHGQLNLRVRCAGDVEIDPHHSVEDVGIVIGMAVREALGDRAGIARYGHAYAP
                     LDEALARTVIDVSGRPYFVFVGDLPEPVIGADFAASLVEEFWRAFATNAGFTMHIDLL
                     RSRNSHHAAEAIFKSAALAIHAATRVSRSTMAIPSTKGVLA"
     gene            complement(676..1782)
                     /gene="hisC"
                     /locus_tag="VHR64_08440"
     CDS             complement(676..1782)
                     /gene="hisC"
                     /locus_tag="VHR64_08440"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX3302893.1"
                     /translation="MTATPPTSLDLTSLVRREVRNLPSYVPATGAAKKPDRLIRLDMN
                     ESPFGPSPRTRAALADFVQTNRYPDFAQSALRSALSSYTGVPMEQIVCGAGLDDVFTS
                     LAHLLIEPGDEVVISEPTFGVYRPLFSMHGATAVNVPLTTGFSLQPDRVVEAVNARTK
                     LVIICSPNNPTGNLFPQEAIESICAQARCLVAIDEAYAEFAGVSQIPLMERFPNVMIL
                     RTMSKWAGLAGMRVGYGLVPTELVGAFSHVVPPFHNVALASSEAAIAAIDDRDFLMKQ
                     VAAICDYRDALISDIGQVAGLEPLPSVTNFILVRTRFPDARPLVKAIAERGVLIRGYG
                     DPLLQPYFRVTVGHPEENDLFLRALRESIEEMPA"
     gene            complement(1793..>2414)
                     /gene="hisG"
                     /locus_tag="VHR64_08445"
     CDS             complement(1793..>2414)
                     /gene="hisG"
                     /locus_tag="VHR64_08445"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF013773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX3302894.1"
                     /translation="EELVALGFGYCSLVVAVMRDSSYQSIEDVKNVKLATSYPNSARR
                     FFAEMGASPEIITLSGSVEMAPGLGLAEGIVELTATGSTLILNDLRQLGTILESEAVL
                     VANKASLEDPEKAQQIDRLLLRINAVRAARRFKYLMMNAPESALEDIKRIVPGLKSPT
                     VVPLQIPGWVAVHMAIQEDIFWESIEQLRAAGATEILVSSLDKLLL"
BASE COUNT          473 a          741 c          776 g          424 t
ORIGIN      
        1 acttcagccc cggctgcttc gagtccgcgg gtgatcgatc ggatattgcc ggcaccatag
       61 tcgatcacgc cgatcacgcg aggaccccct tcgtggacgg gattgccatc gttgagcggc
      121 tgacccgggt tgcggcatgg attgccaacg cggccgactt gaagatcgcc tccgcggcgt
      181 gatgcgagtt tcgcgagcgg agcagatcga tgtgcatcgt gaatccggca ttcgtggcga
      241 atgcgcgcca gaactcttcg accaggctgg cagcaaaatc ggccccgatg accggctcgg
      301 gcaaatcgcc aacaaatacg aaatagggcc gcccggaaac atcgatcacc gtccgtgcca
      361 gggcttcgtc gagcggcgcg taggcatgtc cataccgagc gataccggcc cggtcgccga
      421 gtgcctcgcg cacggccatg ccgatcacga tgccgacatc ctcgacggaa tggtgcgggt
      481 cgatctcgac atcgccggcg cagcgaaccc gcaagttgag ctgtccatgg cgaccgagcg
      541 cgtcgagcat gtggtcgaga aagtgcacgc cggtttcgac gtcgaccgag ccggcgccat
      601 caagctcgac tgtgaccgtg atctcggtct cgcgcgtctt gcgctcaatc gttcgtgtcc
      661 ggattggcat ggcggtcatg ctggcatctc ctcgatcgat tcgcgaaggg cacgtaggaa
      721 caggtcgttc tcttcgggat gaccaaccgt cacccggaag tagggctgca ggagcgggtc
      781 gccatagccg cggatcagga caccccgctc tgcgatcgcc ttgaccaacg gccgtgcatc
      841 cggaaatcgc gtgcggacca ggatgaagtt ggtcacggac ggcagcggct cgagaccggc
      901 gacctgtccg atgtcggaaa tgagtgcatc tcggtagtcg cagatcgcgg cgacctgctt
      961 catcaggaaa tcgcgatcgt cgatagcggc aatcgcggcc tcggacgacg ccagggcgac
     1021 attatggaag ggaggcacaa catggctgaa agcgccgact aactccgtgg gaaccagtcc
     1081 atacccaacg cgcatacccg ccaaccccgc ccacttgctc atcgtgcgca ggatcatgac
     1141 gttcggaaac cgctccatga gcgggatctg cgatacgccg gcgaactcgg cgtaggcctc
     1201 gtcgatcgcg accaggcagc gtgcctgtgc gcagatcgac tcgatcgcct cctgtgggaa
     1261 gaggttgccg gtcggattgt tcggcgaaca gatgatgacc agcttggtgc gcgcattgac
     1321 cgcctcgacg acgcgatcgg gctgcaagga gaaccctgtc gtcagcggca cgttgactgc
     1381 ggttgcgccg tgcatggaga aaagcggtcg atagacgccg aaggtcggct cgctgatcac
     1441 gacctcgtcg ccgggctcga tcagcaggtg cgcgagtgag gtgaagacgt catccaggcc
     1501 cgcgccgcag acgatctgct ccatcggcac gccggtatag ctcgagagag cggaccgaag
     1561 cgccgactgc gcgaaatcgg ggtagcggtt cgtctgtacg aaatcggcca gcgctgccct
     1621 ggtcctcgga gacggtccga atggcgactc gttcatatcg agccgaatca ggcggtccgg
     1681 cttcttggcc gctccggtgg cagggacata gctcggcaaa ttccgcactt cgcggcggac
     1741 gagcgatgtt agatcgaggc tggtcggcgg cgttgcagtc atcgatccgg cctcacagca
     1801 ataacttgtc gagggatgag acgaggattt cggtcgcccc ggccgcccgc aattgctcga
     1861 tcgactccca gaagatgtcc tcctggatcg ccatgtgaac tgcaacccaa cccgggatct
     1921 gaagcggaac aacggtcggc gacttcaagc cagggacgat ccgcttgatg tcctcgagcg
     1981 ccgattcagg cgcgttcatc atcaggtact tgaagcggcg agccgcgcgc acggcgttga
     2041 tccggaggag gaggcgatcg atctgctgcg ccttctcggg atcttcgagc gacgccttat
     2101 tcgcaacgag aaccgcctcc gattcgagga tcgtcccgag ctggcggaga tcattcagga
     2161 taagggtcga accggtcgcg gtcagctcca cgatgccctc ggccagaccg agacctgggg
     2221 ccatctcgac cgatccgcta agcgtgatga tctcggggct ggctcccatc tccgcgaaga
     2281 atcggcgggc cgagttggga taggacgtgg cgagtttgac attcttgacg tcctcgatcg
     2341 attggtacga cgagtcccgc atcacagcga cgaccagcga gcaatagccg aagccgagcg
     2401 cgaccagttc ctcg
//
LOCUS       DATAOZ010000328         9812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2767543, whole genome shotgun sequence.
ACCESSION   DATAOZ010000328 DATAOZ010000000
VERSION     DATAOZ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2767543"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1125
                     /locus_tag="VHR64_08450"
     CDS             175..1125
                     /locus_tag="VHR64_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302895.1"
                     /translation="MNAFIIRRLLGAIPLLLGISILSFIFMQLAPGGPDTLLAKNGRM
                     SQAQLAAIRENLGLDEPVQVQLWTWIKNLFQGDLGTSFSQQRPVRDVIFDVFPNTVYL
                     MAVALTISLLIAVIFGIGAAINQYGWFDNVTSFVAYFGLSMPVFWFGLMLQIMFAVKL
                     GWLPSAGMHSATGGGPFDLIKHMILPALTLAIGSVGSWSRYLRSSTLEVLGHDYVRTA
                     RAKGLREQAVLTKHVLRNAVIPFLTVVAIDIPLYLTGAVVTETVFSWPGMGRLFFDSL
                     GKRDYPVLMGILLLGAFLIVIGNLLADLLYALLDPRISLK"
     gene            1122..2033
                     /gene="opp4C"
                     /locus_tag="VHR64_08455"
     CDS             1122..2033
                     /gene="opp4C"
                     /locus_tag="VHR64_08455"
                     /inference="COORDINATES: protein motif:HMM:NF045476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HEX3302896.1"
                     /translation="MSTAESTLATVDRRSMQQVHTKPMSPWRIVWRRLKRSRIALTGS
                     IVFGLVLLACLLAPVIAPYGKNDIDLFNITAPASSDHWLGTDPLGRDVVTRLLYGGQL
                     SLLIGVGSAVIAVAIGIVVGAVAGYYGGWTDSILMRFVDLMLAFPSIFLLLIVAAILD
                     GITVANVIIFLAAFGWMWLARIIRGEFLSLKQREFVEAARMIGVPSRRIIMRHLLPNV
                     TGPIIVALTLDIALYILAEATLSFLGFGVKPENPTWGNMLNEARTYALTRPLLVIAPG
                     LTITLVVLAINFIGDGLRDAFDPTSGR"
     gene            2375..3016
                     /locus_tag="VHR64_08460"
     CDS             2375..3016
                     /locus_tag="VHR64_08460"
                     /inference="COORDINATES: protein motif:HMM:NF017697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HEX3302897.1"
                     /translation="MIDTYGSVAELLDRERHYVEIDTRVEGRSRIAVLAPHAGQIEPF
                     TGEIARAVAGKEHRYYGFAGTAPGENRRLHVTSTRFDEPALRAVLLGARTALSIHGTS
                     GDDVAATLIGGVNRTLANVIRRNLEQAGFVVMDAPAHLAGTDPRNVVNRVPEGGVQLE
                     LTGKLRQELRKGFLRRVRFDCYVQAIRAALAEHELCPGPRWETLRTQPASPAA"
     gene            complement(3151..4086)
                     /locus_tag="VHR64_08465"
     CDS             complement(3151..4086)
                     /locus_tag="VHR64_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302898.1"
                     /translation="MPKLTLPLIGAGGEPVDFVATINSHGVASLPPVKQNAELATELD
                     ITLRLDDGTIRTVHLAQGKPGMVAIDVYGDAPTDIGQVERAVRHILRLDQDLSSFYHA
                     AAQDPQLAWVTSGYGRMMRCQSVFEEVIKTILTTNCNWSATVKMTERLVTEFGEPDPN
                     LDWEPPFGRAFPTPAAMAERHEPFYRETIRAGYRAPHLVKLATAVASGELDLEPLGTA
                     TIDELSDDDLEKQLRALPGVGPYAAAHIMHMLGRCSRLVLDSWTRPTYARLIGVESIA
                     DPEITERFAAYGGDAGLAFWLTVTRPWFEDHEITP"
     gene            complement(4091..5140)
                     /locus_tag="VHR64_08470"
     CDS             complement(4091..5140)
                     /locus_tag="VHR64_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3302899.1"
                     /translation="MDTIDARQQATQTYQTIHNQPDDLRTMFARERGRIADAARVLSE
                     ASRVLTIGIGSSHHASQVSSWLLRAAGIDALPIHAFDFLHYPEQYPIRPGDAAIIFGH
                     SGTTTFTRQALEQLVVEGVPTIAVGATTAEHHGARVFLRTTEAETSTTYTSSHLCAMA
                     VIAQVAAVIGADFGPALADLPGQVELLLSRELEVWPIAEASAGKRIYAYGAGPNAVTA
                     TELMIKVREAAFHTIDGMAAEQFLHGPTVSFNAGDCAVVIHVPGAGQERVAAIAKVNA
                     AMGGAIWIVGSPIPGLTADHFDLPDAPEMISPLLAVVPMQLFASRLAAIQNTNPDNFR
                     RDDPVYAAAFHSVGF"
     gene            complement(5151..6203)
                     /locus_tag="VHR64_08475"
     CDS             complement(5151..6203)
                     /locus_tag="VHR64_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX3302900.1"
                     /translation="MDTPFPTTDSMMYQTIHRQPQDLSRILTDGWADAEQAAAIITGA
                     NRIWITGIGTSYHAALVGEWLLRSTGKDARALTSFDFATYPEQFPLGPTDAVIVQAHT
                     GVKSYSGLALQRAVAAGVPVISIGSDTAEHPGSRLILRTCEREKSAAYTSSHLCAMAR
                     LAQLAVSLGSEQLREPLLALPNQISGVIEREAEIEPIARDAADRHIYAIGAGPNEPTA
                     LEFVIKAREAALQPCDGLGLEQFFHGPIVAVNAGDFGLVVSMHGASAHRTAAIAKALG
                     MIGLAVWSTGDADPEVPGGTHFALPSTHELISPVLTVVPIQIFAYFLAATRGTHPDRF
                     RREDPVYADAFGLLTL"
     gene            complement(6329..8692)
                     /locus_tag="VHR64_08480"
     CDS             complement(6329..8692)
                     /locus_tag="VHR64_08480"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3302901.1"
                     /translation="MSSVIEHDASAGVRGLPHATTSLVGRTGEAAAILERISDSDARL
                     ITLVGPGGVGKTRLASYFALQALDKFPDGATFVNLAAISDPALVPTVIGATLGLPDRG
                     PSSCSALLINFVTPKRMLLVLDNVEHLLPGVALISDLLAAAPGLTVLATSRVLLRLSG
                     EHAYPVPPLSVPNVRAGMSLDQLTQSDAVRLFLDRARAVRPALALNDETAAAIARICQ
                     LLDGLPLAIELAAARSNVLSPQAMVSRLERRLPLLTGGARDRPLRQQTMQDAIAWSFD
                     LLTPDEQALFRRLSIFVSSFTLEAAEAVTGAFDRIGIDILEGAASLVDKSLLISIDQE
                     GESPRFAMLETVRAFGLEQLRQSGEADHAAEAHAAWFLHLASLAEADSYTSRHPAWIV
                     KLEPDRPNFRATLAWLREHDPGGGFVKLSGDLGRVWYKWERYTEGLRWLEEAAKIARS
                     QPPSRARALILDNLGKLNSILGKNSQSIEYFTESLEAWTELGDLLGIATQTIILGEGY
                     RLSERDDEAIRHYERGLELISEFPDMTQWRSTAMRGLATVELRRENIDRAEELLTEAF
                     AIAQESEMPWTVATAHHGLGQVASMRGRHGEAIAHFVESMRIMRDLNDQISLLFTIPA
                     LAEALVHAGQLERAARLFGATDTYLETIPEADGLADLMACHEPAIASVRMRLGGPAFT
                     EAWSIGRGQPASEVVAFAFDEADVAAAAVSIPGPPPGRATLPGGLSEREAEVLRLAAA
                     GLSNGEMAERLYLSPHTIRAHLQRIYAKLEVENRAAAVRFAVENGLV"
     gene            complement(8826..9302)
                     /locus_tag="VHR64_08485"
     CDS             complement(8826..9302)
                     /locus_tag="VHR64_08485"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX3302902.1"
                     /translation="MSSPIIPPADGSQERRNMNAQERTARNKEIVRRFVEEVVNRGRA
                     DLINDLVAADYVFHCPDGDLYGPLGARLNVEELWLAFPDLHMDLTDLVGEGNLVARRF
                     VLRGVHLGPFLGLRPTEDDVSIPGIALDRLANGRIAESWIAFNTLGLVHAGMSVPT"
     gene            complement(9318..>9812)
                     /locus_tag="VHR64_08490"
     CDS             complement(9318..>9812)
                     /locus_tag="VHR64_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX3302903.1"
                     /translation="DSDPLEGVTVTVAIPASEWGVSATTDADGFATFEELGEGEYMVI
                     LDVPGDAAEFKTVFGTVDGFEPRQHDGQNTNQPIVYVGPHEVLNGTFYVIPANAGAEP
                     EPTETPAQPAVTPKATMEPVTQFPNTGAGDTNGSDRSAPVLAMLAVIALVTAGLVVRG
                     RRSA"
BASE COUNT         1648 a         3097 c         3305 g         1762 t
ORIGIN      
        1 ggtcgtcttc ctctggtgga gtaaggattt ctcggccgtg acgcccaaca ttggcgggtt
       61 ctggccgagc ccattcaacc gtctcctgtg gaacgcgcag gagtggtact tcgccgggta
      121 gaaccttcgg tcggcagggc cggcgattca gccggccctg cattgtgagc ggtcatgaat
      181 gcgtttatca tccgccgctt gctcggcgcg attccgttat tgctggggat ctcgatcctc
      241 tcattcatct tcatgcagct cgctccgggc gggccggata ccttgctggc caagaatggg
      301 cggatgagcc aggcccagct cgcggcgatc cgcgagaacc tcgggctcga tgagccagtc
      361 caggtccagc tctggacctg gatcaagaac ctgttccagg gcgatctcgg caccagcttc
      421 tctcagcagc gacctgtacg ggatgtcatc tttgacgtat tcccgaacac ggtctacctg
      481 atggcggtcg cgctgacgat ctcgttgctg atcgcggtga tcttcggcat cggcgcagcc
      541 attaaccagt acggttggtt cgataacgtg acgtccttcg tcgcgtactt tggtctctca
      601 atgccggttt tctggttcgg tttgatgctc cagatcatgt tcgcagtcaa gctcggctgg
      661 cttccgtcgg ccgggatgca cagcgcgacg ggaggcggac cattcgacct gatcaagcac
      721 atgatcctgc cggcgttgac cctggcgatc ggcagcgtcg ggagctggag ccggtaccta
      781 cgctcctcga cccttgaggt gctcggccat gactatgtgc gcacggctcg ggcgaaggga
      841 ctccgggaac aggcagtcct gaccaagcat gtgctcagga acgcggtgat cccattcctc
      901 acggtcgtcg caatcgacat cccgctctac ctgacgggcg cggtagtgac agagaccgtc
      961 ttctcctggc cgggcatggg ccggctcttt ttcgattccc tgggcaagcg ggattacccg
     1021 gtcctgatgg gtattctgct gctgggcgcg ttcctgatcg tgattggcaa cttgcttgca
     1081 gacctgctct acgcgctgct cgacccacgg atctcgctga agtgagcacg gccgaatcca
     1141 cgttggcgac cgtcgatcgc cgcagcatgc agcaggtgca taccaagccg atgtcgccgt
     1201 ggcggattgt ctggcgacga ttgaagcggt cccggatcgc gctgaccggt tcgatcgtgt
     1261 tcgggttggt gctgttggcg tgcctcctgg cgccggtgat tgcgccttac ggcaagaacg
     1321 acatcgacct cttcaacatc actgcgccgg ccagttccga tcactggttg gggacggatc
     1381 cactcggtcg cgacgtggtc acccgcttgc tctacggcgg gcagctctcg ctgctgatcg
     1441 gcgtcgggtc ggcggtgatc gcggtggcga tcgggatcgt ggtcggagcg gtggccgggt
     1501 actacggtgg ctggacagac tcgattctga tgcggttcgt cgatctgatg ctggccttcc
     1561 cgagcatctt cctgctgctg atcgtagctg cgatcctgga cgggatcacg gttgccaatg
     1621 tgatcatctt tctggccgcg ttcggctgga tgtggctggc ccggatcatc cggggcgagt
     1681 tcctctcgct caagcagcgg gagttcgtcg aggcggcgcg gatgatcggc gtgccgagca
     1741 gaagaatcat catgcgtcac ctgttgccga atgtgaccgg tccgatcatc gtcgccctga
     1801 cgctcgatat cgcgctctac atcctggccg aggcgacgct ctccttcctc ggattcggcg
     1861 tcaagccgga gaacccgacc tggggcaaca tgctgaacga ggcgcggacc tacgccctga
     1921 cccgcccgct gctggtaatc gcgccgggtc tgacgatcac gctggtcgtg ctggcaatca
     1981 acttcatcgg cgacggcctc cgcgacgctt tcgacccaac gtctggtcga tagccttatc
     2041 aaacatcatg ggaatgcgta gtggcggggc tctgtacccg cctgggtccg ttcgacagat
     2101 gtgttggcac ccacgcaaga tgttctcccc ggttccacgc cggtgcgatg gtcggctcca
     2161 aaaatcgggt cggttggcaa gtccaagcgg gtacagagcc ccgccactac gtctcattgt
     2221 ctcggctttg tcacgggtgg attggccaaa tggtcttgac gggacatccg ttagtccggt
     2281 tgatggtttg gacgaagatg tgctttgcac tacatcttgg gctaggcaga atgggggaca
     2341 ggaacgccgt ctgcccgcga ggatgttcgc gtcaatgatc gatacctacg gttccgtcgc
     2401 tgagctgctc gatcgcgagc ggcactatgt tgaaatcgat acccgggttg agggccggag
     2461 ccggatcgcc gtgctcgctc cacacgccgg gcagatcgaa ccgttcactg gggagatcgc
     2521 gcgggcggtc gccggcaagg agcaccgcta ctacggcttt gccggcaccg caccgggcga
     2581 aaaccgccga ctccacgtaa cctcgaccag gttcgacgag ccggcgttgc gggcggtgct
     2641 gctcggggcc aggacagcgc tctcgatcca cggcacgagc ggcgatgacg tggccgcgac
     2701 attgatcggc ggcgtgaacc gaacattggc caacgtgatc cgccgtaacc tcgagcaagc
     2761 cggcttcgtc gtaatggacg cgccggcaca cctggccggg accgatcccc gcaacgtcgt
     2821 caaccgcgta ccggagggcg gcgtccaact cgagttgacc ggcaagctgc ggcaggagtt
     2881 gcgcaaggga ttcctgcggc gggttcgctt cgactgctac gtgcaggcga tccgggccgc
     2941 gctggccgag cacgagctct gcccgggtcc gcgttgggaa acgctgcgca cgcagcccgc
     3001 ttccccagcc gcctagcgaa ttcaccgagc cctttggcgt agcgcggctc gtccctgggc
     3061 cacttcggtg tcgagcaggt aggttgagga cgcaggtctg ctccatcaat cgcgcgtccc
     3121 gtcatcccaa ccggccacgg ggggccggcg ctacggcgtt atttcatgat cttcgaacca
     3181 cgggcgggtg acggtcaacc agaacgccaa gccggcgtcc ccgccgtagg cggcgaaccg
     3241 ctcggtgatc tccggatcgg caatcgattc gacgccgatc agccgggcgt acgtcgggcg
     3301 ggtccagcta tcgagcacaa ggcgggagca gcgaccgagc atgtgcatga tgtgcgcggc
     3361 ggcatacggc ccgacgcccg gcaacgcgcg gagttgcttc tcgaggtcgt cgtccgacag
     3421 ctcatcgatc gtcgccgttc cgagcggctc gaggtccagc tcgccgctgg ccacggcggt
     3481 cgccagtttg acgaggtgcg gcgcgcgata cccagcccgg atcgtctccc ggtagaacgg
     3541 ctcgtggcgc tcggccatgg cggccggagt cggaaaggca cggccgaacg gcggctccca
     3601 atcgaggttc ggatcaggct cgccgaactc ggtgaccaac cgctcggtca tcttcacggt
     3661 cgccgaccag ttgcagttcg tggtcaggat cgtcttgatc acttcctcga agaccgactg
     3721 gcaacgcatc attcgcccgt acccgctggt gacccaggcg agctgcgggt cctgcgccgc
     3781 agcgtgatag aagctgctca gatcctggtc gagccggaga atgtgccgga ccgctcgctc
     3841 aacctgaccg atatcggtcg gggcgtcccc atagacatcg atcgcgacca tgcccggctt
     3901 gccctgcgcg aggtggaccg tccggatcgt gccgtcgtca agccggagcg tgatgtccag
     3961 ctcggtggcg agctcggcgt tctgcttgac cggaggcagg gacgcaacgc cgtggctgtt
     4021 gatcgtcgcc acgaagtcga ccggctcccc gcccgcgcca atcagcggga gtgtcagctt
     4081 gggcacagtg ctagaacccg accgagtgga acgccgcggc gtagaccggg tcgtcgcgcc
     4141 ggaagttatc cgggttggtg ttctggatcg cggccaggcg gctggcgaag agctgcatcg
     4201 gaaccaccgc caacaggggc gagatcatct ccggcgcatc cggcaaatcg aagtgatcgg
     4261 cggtcaggcc cggaatcgga ctcccgacga tccagatcgc gccgcccatc gccgcgttga
     4321 ccttcgcaat cgccgcgacc cgttcctgac cggcaccggg gacatggatc acgaccgcgc
     4381 aatcgcccgc gttgaacgag accgtcgggc catgcaggaa ctgttcggcc gccatgccgt
     4441 cgatcgtgtg gaaggccgcc tcgcggacct tgatcatcaa ctcggtggcc gtgaccgcgt
     4501 tcggacccgc accgtaggca tagatccgct tgccggccga cgcctcggcg attggccaga
     4561 cctccagctc gcgcgagagg agcagctcga cctgaccggg cagatcggcg agcgcgggac
     4621 cgaagtccgc gccgatcacg gccgcgacct gggcgatgac cgccatcgcg cagaggtgcg
     4681 agctcgtgta ggtggtcgac gtctccgcct cggtcgtgcg caggaagacg cgggcgccgt
     4741 gatgctcggc cgtcgtcgcc ccgactgcga tcgttggcac gccctcgacc accagctgct
     4801 cgagggcctg gcgggtgaag gtcgtggtgc cggaatgacc gaagatgatt gccgcatcac
     4861 ccggccggat cgggtactgc tcggggtagt gcaggaagtc gaaggcgtgg atcgggagcg
     4921 catcgattcc cgccgctcgc agcagccagg agctgacctg cgaggcatgg tgactggagc
     4981 cgatgccgat cgtgaggacc cggctcgcct cgctgagcac gcgcgcggcg tcggcaatgc
     5041 ggccgcgctc gcgggcgaac atggtccgca gatcgtccgg ttgattgtgg atcgtctggt
     5101 aggtctgggt cgcctgctgg cgcgcgtcaa tcgtgtccat gggaatactc ctagagcgtc
     5161 agcaggccaa aggcatcggc gtagaccgga tcttcgcggc ggaagcgatc ggggtgggtg
     5221 ccccgggtcg cggccaggaa gtaggcgaag atctgaatcg ggacgacggt caggaccggc
     5281 gagatcagct catgcgtcga tggcagggcg aagtgcgtcc cgcccggcac ctcgggatcg
     5341 gcatcgccgg tcgaccagac ggcgagccca atcatgccga gcgccttggc aatcgccgcg
     5401 gtacggtggg cggaggcccc gtgcatcgag acgacaagcc cgaagtctcc cgcgttgacc
     5461 gcgacgatcg gaccgtggaa gaactgctcg aggccgagac cgtcgcacgg ctggagtgcg
     5521 gcctcccggg ccttgatcac gaactcgagc gccgtcggct cattcggtcc ggcgccgatt
     5581 gcgtagatat ggcggtcggc ggcatcccgc gcgatcggct cgatctcggc ctcgcgctcg
     5641 atcacgccgg aaatctgatt gggcagggcc aacagcggct cgcgcagctg ctccgagccg
     5701 agtgagacgg cgagctgcgc cagccgggcc attgcgcaga ggtgcgatga ggtgtaagcg
     5761 gccgattttt cgcgctcgca ggtgcgcagg atcagccgcg acccgggatg ctcggccgta
     5821 tcgctgccga tcgagatgac cggtacgccg gctgcgaccg ctcgctggag ggccagcccg
     5881 gagtagctct tgaccccggt gtgggcctgg acgatgacgg cgtcggtcgg gccgagcggg
     5941 aactgttccg ggtaggtcgc gaagtcgaac gaggtgagcg cccgggcatc cttgccggtc
     6001 gagcggagca gccattcgcc gacgagcgcc gcgtgatagc tcgtgccgat gccggtgatc
     6061 cagatccggt tcgcgccggt gatgatcgcg gcggcttgct cggcatcggc ccagccgtcg
     6121 gtcaggatcc ggctgagatc ctgcggctgg cgatgaatcg tctggtacat catcgagtcg
     6181 gtggtcggaa atggtgtatc catgggccct ccggtatatc tacgtttcgg cactctatca
     6241 gtcgagaagt cgaggaggcg agaagagggg gacgaaggca cacaggcgca gagcagggga
     6301 tagaccgctt tttgccccgt cccctggatc agacaaggcc gttttcgacg gcgaagcgga
     6361 cggcggcggc ccggttctcg acctccaact tggcgtaaat gcgctgcaga tgggcgcgaa
     6421 tcgtgtgcgg gctgagatac agccgctccg ccatctcgcc attcgagagg ccggcagcgg
     6481 caaggcgcag cacttcagcc tcccgctccg agagcccgcc cggcagcgtc gcccggcccg
     6541 ggggaggccc tggaatagac acggcagccg ctgccacatc cgcttcatcg aaggcaaagg
     6601 caaccacctc ggacgccggt tgccctcgcc cgatcgacca ggcctcggta aaggctggcc
     6661 cgccgagtcg catgcgaacc gaggcgatgg ctggttcgtg gcaagccatg agatccgcca
     6721 ggccatcggc ttcgggtatg gtctctagat acgtgtcggt cgctccgaag agccgggcag
     6781 cccgctccag ttggccggcg tggacgaggg cctcggccag tgccgggatc gtgaacagca
     6841 acgagatctg gtcgttcagg tctcgcatga tacgcatgct ctcaacgaaa tgcgcgattg
     6901 cctctccatg tcggccgcgc atgctcgcta cctggcccaa gccatggtgc gcggtggcga
     6961 ccgtccaggg catctcgctt tcttgcgcga tcgcgaaggc ctcggtcagg agctcttcgg
     7021 cccgatctat gttctcgcgt cgtagctcga ccgtggcaag gccgcgcatc gcagtcgacc
     7081 gccactgggt catgtccggg aactcgctga ttagctccag cccccgctca tagtggcgga
     7141 ttgcctcgtc gtcgcgctcc gagaggcgat acccctcgcc gaggatgatt gtctgagtgg
     7201 cgattccgag caggtcgcca agctcggtcc aggcctccag gctctcggtg aagtactcga
     7261 tggactgcga gttcttgcca aggatcgagt tgagcttgcc caggttgtcg aggatcaggg
     7321 ccctggctcg ggacgggggc tgcgaccgag cgatctttgc cgcctcctcg agccagcgca
     7381 gtccctcggt gtagcgttcc cacttgtacc agacgcggcc aagatccccg gaaagcttga
     7441 cgaagccgcc gccggggtcg tgctctcgta accaggcaag cgttgcccgg aaattaggac
     7501 ggtccggctc gagcttcacg atccaggccg gatggcgcga ggtatatgag tcggcttcgg
     7561 ccaggcttgc caggtggagg aaccaggcgg cgtgcgcctc ggccgcgtga tcggcctcac
     7621 cgctctgcct gagctgctcg agaccaaacg cgcggactgt ttccagcatt gcgaagcgag
     7681 gcgactcccc ttcctggtcg attgagatga gcagactctt gtcgacgagt gacgcagccc
     7741 cttccaggat gtcgattcca atgcgatcga acgcaccggt caccgcctcg gcggcttcga
     7801 gcgtgaaact gctgacgaag atcgacaacc ggcggaacag cgcctgctcg tcgggtgtga
     7861 gcaggtcgaa ggaccaggcg atcgcgtcct gcatcgtctg ctggcggagc ggccgatcac
     7921 gcgcaccgcc ggtcaatagc ggcaaccggc gttcgaggcg gctgaccatt gcctgcggcg
     7981 acaggacatt cgaacgggcg gcggccaact cgatcgcgag cggcaaccca tcgagcagtt
     8041 ggcagatgcg ggcgatcgcc gccgcggttt cgtcattcag cgcaagggca ggacggacag
     8101 ctctggcgcg gtccaggaac aggcggacgg cgtcgctctg ggtgagctgg tcgagcgaca
     8161 taccggcgcg gacgttcggt acgctcaacg gcgggacagg gtatgcgtgc tctcctgaca
     8221 agcgcaggag gacccggctc gtcgccagca cggtcaatcc cggggccgcg gcaagcaagt
     8281 ccgagatcag cgccacaccg ggcaagaggt gctcgacatt gtcgaggacg agcagcatcc
     8341 gtttcggagt gacgaagttg atcaacagag cgctgcatga gctcggaccc cggtccggca
     8401 agccgagcgt cgcgccgatc accgtcggaa ccagcgcggg atcggagatg gcggcaaggt
     8461 taacgaatgt cgcgccatca ggaaacttgt cgagtgcctg cagggcgaag tagctcgcca
     8521 ggcgtgtctt gccgacacca cccgggccga ccaacgtgat caaccgcgcg tctgagtcag
     8581 agatacgttc gaggatcgcc gcggcctcgc cggtccggcc gacgagtgag gttgtggcat
     8641 gcggcaaacc ccgaacgccg gcggacgcat cgtgctcaat gacgcttgac atagaggcga
     8701 ttctagcgcc cattgcggag tttcggaata gtcctgagaa atgggagcgc ctgcatcgtg
     8761 gctggtcgat gcaggcgcgg ggcaagggag gaaattcgag gcggctggct ggaaccgcct
     8821 cggatctatg tggggacgct catcccggcg tgaaccaggc cgagcgtgtt gaaggcgatc
     8881 cacgattcgg cgatccggcc gtttgcgagt cgatccaggg caatccccgg gattgagaca
     8941 tcgtcctcgg tgggcctcag cccaaggaat gggccgaggt ggacccctcg gagaacgaag
     9001 cgccgcgcaa ccaggttccc ttcgccgacg agatcagtca gatccatgtg caggtccggg
     9061 aaggcaagcc acaactcttc gacgttgagc cgggcgccga gcgggccgta gaggtcccca
     9121 tccgggcagt ggaagacgta gtcggcagca accaggtcat tgatcaggtc ggctcgacca
     9181 cggttgacga cctcttcgac gaaccggcgc acgatctcct tgttccgggc cgttcgttcc
     9241 tgcgcgttca tgttcctgcg ctcctgcgag ccgtcggccg gagggatgat gggtgagctc
     9301 atccctccga tccggcgtta cgcgctcctt cggccgcgga cgacgagtcc cgcggtgacc
     9361 agggcgatca cggccagcat ggccaggacc ggcgccgatc gatcgctgcc gttcgtgtca
     9421 cccgcgcccg tgttcggaaa ctgcgtgacc ggctccatcg tcgccttcgg cgtaacggcg
     9481 ggctgggccg gcgtctcggt tggctcgggc tccgcaccgg cgttggccgg gatcacgtag
     9541 aaggtgccgt tcagtacttc gtgcgggccg acgtagacga tcggctggtt cgtattctgg
     9601 ccgtcgtgct ggcgcggctc gaagccgtcc acagtgccga agaccgtctt gaactctgcc
     9661 gcgtcaccgg ggacatcgag gatgaccatg tactcgccct cacccaactc ctcgaatgtc
     9721 gcgaacccgt ccgcgtccgt cgtggcggag acgccccatt cgctcgccgg gatggcgacc
     9781 gttacggtga cgccctccaa cgggtcgctg tc
//
LOCUS       DATAOZ010000329         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3030502, whole genome shotgun sequence.
ACCESSION   DATAOZ010000329 DATAOZ010000000
VERSION     DATAOZ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3030502"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VHR64_08495"
     CDS             <1..880
                     /locus_tag="VHR64_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3302904.1"
                     /translation="VIGAGPAGLIATALLAKRGESVTLIDEQAEAGGHLRYDQYPVGS
                     DRHESDSWLTEIRGTVDASDATMLQSAIIWAAFRVGNGFELAVNHAGAEQTVRADHLV
                     VATGTTDLPLVIPGATLPGVMTSRAVRILLNRHGVLPGQRLVIAGTGSEADRLQGDLT
                     AAGADATIAAPDTIAAVSGVRGVESVRLTDGHTVEADCLIVARGEVPDVQLPGMLEAP
                     RTYDPAIEGWRLTPGADVPGLTVIGGALLGAAGPDQVVQSAVEAADRISPTGPGIGDI
                     RLDITDAILRWEAVQG"
     gene            877..1158
                     /locus_tag="VHR64_08500"
     CDS             877..1158
                     /locus_tag="VHR64_08500"
                     /inference="COORDINATES: protein motif:HMM:NF037640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3302905.1"
                     /translation="MSGGETYLCRCECISTGQVDAAIAEGNLTINDIKRRTRAGMGIC
                     QGIFCLGPMAQQLVAAGIEPDTIEPMTSRPPTRLVPVGLAAAHAPEQDA"
     gene            1155..1436
                     /locus_tag="VHR64_08505"
     CDS             1155..1436
                     /locus_tag="VHR64_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302906.1"
                     /translation="MSHEHGWMRPALDALFDSVSVEEAREAVLSDERRTELRSTLNQD
                     LGTAPIPDGQYADNLLQGLLALRMKFDRAEAIQKLELNLSMRTGRGAHR"
     gene            1433..2515
                     /locus_tag="VHR64_08510"
     CDS             1433..2515
                     /locus_tag="VHR64_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3302907.1"
                     /translation="MTPMSARQTAINTAVILLVVFVAWLLVQVRSTIVLLVIGILFAA
                     AIEPLVNRLRRRGFKRGQAILTIYATLLMTVVLALLLVVPAIARQTTSLIDNIPDILQ
                     SSRDRAYDINNDGIRNAVLRGINEVDDFYNDAKTNPDTGLASDAAIALVTTVGGALAS
                     IVTVLVVAFYWLTEKSTIKQVTLRKVSPRERDRAFTIWEEIERRIGGWARGQMTLCLI
                     IGVISTIGYFALGLNYWIALGIWAGITELIPFIGPILGGAAAFAVALTDSWQKALMVV
                     AFVVVLQQFESAVLVPRVMRSSVGMSPLTVLLAVLVGSTLGGPLGAVLAIPVGAAVQA
                     IVQEVVRDQVDQSEKEEQQVVLAGFD"
     gene            complement(2512..2991)
                     /locus_tag="VHR64_08515"
     CDS             complement(2512..2991)
                     /locus_tag="VHR64_08515"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX3302908.1"
                     /translation="MAMWSKLKKAKAANVGDAAPDFELTGTTGPGLKLSDLRGKKRAV
                     LFFYPQDCTSGCLAQLTEAGQVMDEFRALDTEPFGVNEAEAESHQVFLDQLDIPFDLL
                     VDRNFGVSRAYDALKEDQDRISRTVVVVGKNGKVIFRAAGAPPADEILREIANAKDS"
BASE COUNT          551 a          975 c         1110 g          594 t
ORIGIN      
        1 ggtcattggc gctggaccag cgggattgat tgcgacagcg ctgttggcga agcggggcga
       61 gtcggtcacg ctgatcgacg agcaggcgga ggcaggcggc cacctcagat acgaccagta
      121 tccggtcgga tcggatcggc acgagtcgga cagctggctc accgagatcc gcggtaccgt
      181 cgacgcatca gatgcgacca tgcttcaatc ggcaattatc tgggcggcgt tccgggtggg
      241 caatggcttc gagttggcgg tcaatcacgc cggcgccgag cagaccgtgc gtgctgacca
      301 cctggtcgtc gcgacaggca cgaccgatct tccactggtc atccccggcg caaccctgcc
      361 aggcgtcatg acgagccggg ccgttcgcat cctgctcaat cggcacggtg tgcttcccgg
      421 gcagcgactg gtcattgccg gtacggggtc tgaggctgat cgacttcagg gcgacctgac
      481 ggccgcaggg gccgatgcga ctattgccgc gccggacacg atcgcggcag tttccggagt
      541 tcgaggcgtg gagtcggtcc ggctcacgga tggccacacg gtcgaggccg actgtttgat
      601 cgtcgcccgg ggtgaggtgc cggacgtgca gctgccgggc atgctggagg cgccgcgaac
      661 ttacgatccc gcgatcgagg gctggcgtct gaccccgggc gcggacgtgc ccggattgac
      721 ggtaatcggc ggcgcgctgc tgggcgcggc gggaccggac caggtcgtgc agagcgcggt
      781 cgaggcagcg gatcggatct caccgaccgg acctggaatt ggcgatattc gattggacat
      841 caccgacgcg atccttcggt gggaggctgt acagggatga gcggcggcga aacttatttg
      901 tgccgatgcg agtgcatctc gaccgggcag gtcgatgcgg caattgccga aggcaacctg
      961 acgatcaacg acatcaagcg acgcacgcgg gcaggtatgg gcatctgcca gggcatcttc
     1021 tgcctcggcc cgatggcgca gcaactggtc gcggccggca tcgagcccga cacgatcgag
     1081 ccgatgacct cgcggccgcc aacccggctc gttccggtcg ggctcgcggc agcccatgcc
     1141 cctgagcagg acgcgtgagc cacgagcacg gctggatgcg accggccctc gatgcgctct
     1201 tcgactcggt cagtgtcgag gaggctcgcg aggcggtgct gagcgatgag cgccggaccg
     1261 agttgcggag cactttgaat caagatctcg gtactgcgcc aattcccgac gggcaatatg
     1321 cggacaatct gctgcagggg ttgctggcgc tgcggatgaa gttcgatcgt gccgaggcga
     1381 tccagaagct cgaactcaac ctatcgatgc gtactggccg aggtgctcat cgatgacccc
     1441 aatgtccgca cgccagacgg cgatcaacac ggccgtcatc ctgctggtcg tgttcgttgc
     1501 ctggctgctt gtgcaggtgc ggtcgacaat cgtcctgctg gtgatcggca tcctgttcgc
     1561 ggcggcgatt gagccgctcg tcaaccgctt gcgtcgcaga ggattcaagc gcggacaggc
     1621 gatcctgacg atctacgcga ccttgttgat gacagtcgtg ctggcgctgt tgctggtggt
     1681 cccggcgatt gcgcggcaga caacgtcgct gatcgacaac atccccgaca tcctccagtc
     1741 gtcccgcgat cgggcatatg acatcaacaa tgacggcatt cgcaacgcgg tgttgcgtgg
     1801 gatcaacgaa gtcgacgact tctacaatga cgcgaagacc aaccccgata cgggcctggc
     1861 cagcgacgcg gcgatcgccc tggtgacgac cgtcggcggc gcgctggcgt cgatcgtgac
     1921 ggtgctggtc gtggcgttct attggctaac cgaaaagtcg accatcaagc aggtcacgct
     1981 ccgcaaggta tcaccaaggg agcgggatcg ggcgttcacg atctgggaag agatcgaacg
     2041 caggatcggc ggttgggcgc gcgggcagat gacgctctgc ctgatcatcg gagtgatctc
     2101 gacgattggc tactttgcgc tcgggctgaa ctactggatc gccctcggca tctgggcggg
     2161 catcaccgag ctgatcccgt ttatcggacc gatcctgggc ggcgcggcgg cgtttgcggt
     2221 ggccctgacc gattcctggc agaaggcgtt gatggtggtc gcctttgtcg tggtgctcca
     2281 gcagttcgag agcgcggtgc tcgtgccccg ggtgatgcgc agctcggtcg gcatgtcgcc
     2341 gctgaccgtg ctcctggcgg tgctggtcgg gtcaacgctg ggtggaccgc tcggcgccgt
     2401 gctggcgatc ccggtcggcg cggcggtcca ggcgatcgtg caggaagtgg tccgggatca
     2461 ggtcgaccag tcggagaagg aagagcagca ggtcgttctg gccgggttcg attagctgtc
     2521 cttcgcgttc gcgatctcgc gcaggatctc gtccgccggc ggtgcaccgg cagcccggaa
     2581 gatgaccttg ccgttcttgc cgacgaccac cacggtccgc gagatgcgat cctggtcctc
     2641 tttcagcgcg tcgtaggccc gcgagacacc gaagttgcgg tcgaccagca gatcgaatgg
     2701 gatatcgagc tggtcgagga aaacctggtg gctctcggct tccgcttcgt tgacgccgaa
     2761 cggctcggta tcgagcgcgc ggaactcgtc catcacctga ccggcttcgg tgagctgggc
     2821 caggcagccg ctggtgcaat cctgcggata gaagaagagc acggcccgct tcttgccgcg
     2881 cagatcgctc agcttcagac cgggaccggt cgtcccggtc aactcgaagt caggcgcggc
     2941 atcgccgacg ttggcggctt tcgccttctt gagcttgctc cacatggcca tagaccgcct
     3001 ccttcagcgt tgaacgattc ctgtgttgga ttgtacgccg gctggtcacg acgctagggg
     3061 acggccagca tctggtcgag cgcgaccagg gcatcccgcc tggtctcgtc gtcgaccgaa
     3121 atctgattga cgacctcgcc ctcgaccagg tgctcgagca cccagaggat gtacgccgga
     3181 tggatccggt acatggttga gcaggggcaa atgactggat cgaggcagaa
//
LOCUS       DATAOZ010000330         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2767971, whole genome shotgun sequence.
ACCESSION   DATAOZ010000330 DATAOZ010000000
VERSION     DATAOZ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2767971"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VHR64_08520"
     CDS             <1..202
                     /locus_tag="VHR64_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302909.1"
                     /translation="KSADGVAPTPTGQTSASLVWRPRIIRSWWSVWALVKSESSLSDD
                     PTTDGEATAAYLALIVKPMGSR"
     gene            556..1554
                     /locus_tag="VHR64_08525"
     CDS             556..1554
                     /locus_tag="VHR64_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302910.1"
                     /translation="MVIQPEPMISTLPEQNPDTEGRGIEGRSLSRIAWTRLRQDRVAM
                     TGGAFVVFIILVAIFAPLIVKLLGHAPNEFHQDYAKLLDQNTGGMPKGTFGGISWQYL
                     FGIEPENGRDVFSRVVYGARISLTIGFGAAILSVAIGTALGLVSGFFGGWIDGAIGRA
                     MDLFLAFPLLVFALAIVGVLPDSAFGLSGNGLRVGIMIFVIGFFNWPYIARIIRGQVL
                     SLREREFIDAARSLGARSPHIIVREMLPNLIAPILIYTTLLIPTNVLFEAALTFLGVG
                     IVPPTASWGGMLSDASHYYQTDPAYMFFPGMAIFLTVLAFNLFGDGLRDAFDPKGR"
     gene            1641..>2149
                     /locus_tag="VHR64_08530"
     CDS             1641..>2149
                     /locus_tag="VHR64_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3302911.1"
                     /translation="MAAITVAVALGVTGACGSSNSSNGTSASQPGFNAALSSVFNPSN
                     SKGGTVTMAITEDWDSVDPGDTYYALSWNLVRLYGRSLVMFDPVPGPDGNKLVPDLAT
                     SLGQSSNGGKTWTYHIRQGVKLSDGTELTAADVKYGILRSLDKTVLVNGPTYFNDWLN
                     LPKGYEGPY"
BASE COUNT          359 a          696 c          692 g          402 t
ORIGIN      
        1 gaagtcagcg gacggcgtgg cgcccacccc gacaggccag acgtccgcgt ccctggtctg
       61 gcgcccgcgc attatccgga gctggtggtc cgtctgggca ctggtgaagt cagagagctc
      121 actctccgac gatccgacca ccgatggcga ggcgaccgcg gcctacctcg ccttgatcgt
      181 caagccaatg gggtcccggt gaccgggctc gcgagcggcc ttccggtcgg cggcaatctc
      241 gaacatgcgc accgcaatcg acacgcgctc gcaacgggcc cgcttatacc aaaccggcgg
      301 caaaaccgca ggataaaggt aatatttctg gcatctgcac agcagcgacg tttgtccacg
      361 tgttcggcga caggccgcgc acgactcggc gacccgagtg ccgcctgatc ttgcggtcgt
      421 tcttgcggtc gtacgacggt ggtctcggtt tccgagcttg atgtgagccc ttcgccaatg
      481 cgcgtctcgc gtcctcgctg gctgtagagc tgccgcctgg cgatgtcacg gtcgctggcg
      541 tagggggtgg tgccggtggt catccagccg gagccgatga tctccaccct gcccgagcag
      601 aacccggaca ccgagggccg cgggatagag ggccgctccc tcagccggat cgcctggacc
      661 cggctgcggc aggaccgggt cgcgatgacc ggcggcgcgt tcgtcgtctt catcatcctg
      721 gtcgcgatct tcgcgccgct gatcgtcaaa ctgctcgggc atgcgccgaa cgagttccac
      781 caggattacg cgaagcttct cgaccagaac accggcggta tgccgaaagg tacgttcggc
      841 ggcatcagct ggcagtacct gttcgggatc gagccggaga acggacggga cgtcttcagc
      901 cgcgtcgtgt acggcgcccg gatctcgctc accatcggat tcggtgccgc gatcctgtcg
      961 gtcgcgatcg ggaccgcgct cgggctcgtc tccggcttct tcggcggctg gatcgacggt
     1021 gcgatcggcc gggcgatgga cctgttcctc gcgttcccgt tgctcgtctt tgcgctcgcg
     1081 atcgttggcg tgctgccgga tagcgcgttc gggttatccg gtaacggtct ccgggtcggg
     1141 atcatgatct tcgtgatcgg gttcttcaac tggccgtaca tcgcccggat catccgaggt
     1201 caggtgctgt cattacgcga gcgcgagttc atcgacgcgg ctcgaagcct cggcgcgcgc
     1261 agcccgcaca tcatcgtgcg ggaaatgctg cccaacctga tcgcgccaat cctgatctac
     1321 acgacgctac tgatcccgac gaacgtgctg ttcgaggcgg cgttgacgtt cctcggcgtc
     1381 ggcatcgtgc cgccgacggc gagttggggc ggcatgcttt cggacgcctc gcactactac
     1441 cagaccgacc ccgcgtacat gttcttcccc ggtatggcga ttttcctgac tgtgcttgct
     1501 ttcaaccttt tcggagacgg gctgcgagac gcgttcgatc ccaagggccg ctagagctgc
     1561 atggatgagg gattagccac aggtccgtga cccggaccga accagaggag ggaatggacg
     1621 tgaaacgaag gatgagagcg ttggcggcga tcaccgtcgc tgtggcactc ggggtgaccg
     1681 gggcctgtgg ctcctcgaat tcatcgaacg gcacctcggc gtcgcaacca gggttcaacg
     1741 ccgcgctcag ttcggtgttc aacccgtcca acagcaaggg cggcaccgtc acgatggcga
     1801 tcaccgagga ctgggactcg gttgacccgg gcgacaccta ctatgcgctg tcctggaacc
     1861 tggtccggct ctacggccgc tcgctggtca tgttcgatcc ggtacccggc ccagacggca
     1921 acaagctggt accggatctg gccacgtcgc tcggccagtc gagtaacggt ggcaagacct
     1981 ggacgtacca catccgccag ggcgtcaagc tctccgacgg caccgagctc accgcggccg
     2041 atgtcaagta cgggatactg cggtctctgg acaagaccgt cctggtcaac gggccgacgt
     2101 acttcaacga ctggctgaac ctgcccaagg gctacgaggg cccgtacaa
//
LOCUS       DATAOZ010000331         4518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8819474, whole genome shotgun sequence.
ACCESSION   DATAOZ010000331 DATAOZ010000000
VERSION     DATAOZ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8819474"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VHR64_08535"
     CDS             <1..646
                     /locus_tag="VHR64_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3302912.1"
                     /translation="IIVPPDSYFRRVKEILDHHGILFICDEVQSGFGRCGSSFAIEQF
                     GVDPDILVAAKGIADGFPIAACIATPEIADSLKPGEHLSTYGGNPICCAAASANIDVM
                     LEEDLPGQAARKGERFMAGLHDLAGKHDLVGEVRGIGLMMGMELVTDRKTKAPAKAQA
                     ADIRRICRENGVLVGVGGQLANVVRIQPPLVISDEDLDRTLEVLDQALTQAAKA"
     gene            1054..3774
                     /gene="acnA"
                     /locus_tag="VHR64_08540"
     CDS             1054..3774
                     /gene="acnA"
                     /locus_tag="VHR64_08540"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX3302913.1"
                     /translation="MATRASNEVRDPFGVRGTVEAGGHAYSIYSLPKLTESGVANIDR
                     LPFVVRIMLENLLRHVGTEFATADDVTTFANWDPAKATPDIELAYLPARVILQDFTGV
                     PCVVDLAAMRSAVARMGGDVSKINPLVPVDLVIDHSVQVDRFGTMMAFGQNVAFEYER
                     NRERYMLLRWAQEAMQNFRVVPPGTGIVHQVNLEYLASVVASREIDGEMVAFPDTLVG
                     TDSHTTMISGLGVLGWGVGGIEAEAVLLGQPLYQLMPEVVGVKLSGQLEPGATATDLV
                     LRVTEMLRSFGVVGRFVEFFGAGLSSLPLADRATIANMSPEYGATTGFFPVDDVTLGY
                     LRKTGRSTEQVDLVERYCKQQGLFRTDATPDPVYNAVLELDLGSIRPSVAGPRRPQDR
                     IELDNVKSTFRAAYADRLPSANGEEAPLPGVPIKVGESIAHVRHGSVVIAAITSCTNT
                     SNPSVMLGAGLLAKKAVERGLDVAPYVKTSLAPGSQVVTRYLDQSGLTGYLEALGFHT
                     VGYGCTTCIGNSGPLPGAVAEAVDDNELVVAAVLSGNRNFEGRVHQQVRASFLASPPL
                     VVAYALAGTVDIDLARDPLGIDPNSQPVYLADIWPTRDEVEAVVAENVTSEMFQEEYA
                     TVFDGDDRWKTLPVPTGELYHWDPESTYVQEPPYFIDMSPEPQPVHDIAGARVLVLVG
                     DSVTTDHISPAGAIARTSPAAQYLVNNDVEPRDFNSYGSRRGNHEVMVRGTFANIRLR
                     NLLVPGKEGNLTVYLPTGEEMSIYDASYLYQRENIPLLAIAGKEYGSGSSRDWAAKGP
                     MLLGIKAIIAESYERIHRSNLIGMGVLPLQFKPGENLSTFGLNGTETFAISGIADNLT
                     PRKVMTVTATKSDGSTVSFEAVARIDSKVEVGYYKNGGILQTVLRRLMAS"
     gene            3781..>4518
                     /locus_tag="VHR64_08545"
     CDS             3781..>4518
                     /locus_tag="VHR64_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3302914.1"
                     /translation="MADVQLPRYADYVIVGAGPGGCAAAAELARSGSASVLVLEAGPD
                     YGPFNSGRWPNDLLEAFDLAETHGWGYDSESTFGSRVVGFSRAKVIGGCSSHNGCAAI
                     WGHRLDYDGWAEMGLDGWSTDELLPFFRAANEAMRVAIPGPSHVTPFHQLMLESAAAA
                     EIPLVADLNDLDEPIGMAPSPANIWNGIRWNAAFAFLDPIRDLPTVTIAGDVLVDTVR
                     IENSRATGVHVIHDGASHIIEAGTVVLS"
BASE COUNT          774 a         1529 c         1427 g          788 t
ORIGIN      
        1 gatcatcgtg ccgccagatt cctacttccg ccgggtcaag gagatcctcg accaccacgg
       61 catcctgttc atttgcgacg aagtgcaatc cggattcggc cgctgcggct cctccttcgc
      121 gatcgagcag ttcggcgtcg acccggacat cctggtcgcg gccaagggta tcgccgacgg
      181 cttcccgatc gcggcctgca tcgccacgcc ggagattgcc gacagcctca agccgggaga
      241 gcacctgagc acgtacggcg gcaatccgat ctgctgcgcg gccgcctcgg ccaatatcga
      301 cgtcatgctc gaggaggacc tgcccggtca ggcggcacgg aagggcgagc gcttcatggc
      361 cggcctgcac gacctggccg gcaagcacga cctggtcggc gaggtgcgcg gcatcggcct
      421 gatgatgggc atggagctgg tcactgaccg caagaccaag gccccggcca aggcccaggc
      481 agccgacatt cgccgcatct gccgcgagaa cggcgtcctc gtcggcgtcg gcggtcaact
      541 cgccaacgtc gtccgcatcc agcccccgct cgtgatcagc gacgaagacc tcgaccgcac
      601 cctcgaagtc ctcgaccagg cgttgaccca agcggccaag gcgtagcgcg gggtcgccga
      661 gccgcgccgg cgattaactc atgttctcaa ggaaattggc cgtccgtgcc gcccggggat
      721 aaatgcccgg gctgacccac aagaagccca ctgaaggggc tgccaatttt gtacagcccc
      781 ttcagtgggc ttctgcttgc ttaaccccga cctttagctc ggggcggcgt tggagacctg
      841 gttgccatgc gtcgggctag taatcggact ccaaggttgc actgctctgc gacgcttcaa
      901 ctccgcgcgg ccactgctcg cctgctcgcc tccatatgtc tcccacccac gcccggggtt
      961 cagtgtcctc ccctacaata ggggcataac tgactcgcgg tcggattccc cgctttccac
     1021 gcctccgacc ctattccggg agggctcctt accatggcta ctcgtgcgtc caatgaagta
     1081 cgcgatccgt tcggtgtgcg cggcaccgtc gaggcaggcg gacatgccta ctcgatctac
     1141 agcctgccga aactgactga gtccggcgtc gccaatatcg accggctccc cttcgtggtc
     1201 cggatcatgc tcgagaacct gctccgccac gtcggcaccg agttcgcgac ggccgacgat
     1261 gtcacgacct tcgccaactg ggaccccgcc aaagcgacgc cggacatcga gttggcgtac
     1321 ctgccggccc gggtcatcct ccaggacttc accggcgtcc cttgcgtggt tgacctcgcg
     1381 gcgatgcgat cagctgtggc gcggatgggt ggcgacgtca gcaagatcaa tccgctcgtg
     1441 ccggtcgacc tcgtcatcga ccactcggtc caggtcgatc gcttcggcac gatgatggcc
     1501 ttcggacaga acgtcgcctt cgagtacgag cgcaaccggg agcgctacat gctgctccgc
     1561 tgggcgcagg aggcgatgca gaacttccgc gtcgtcccgc ccggcaccgg catcgtccac
     1621 caggtcaacc tcgagtacct ggccagcgtg gtcgcatcgc gtgagatcga cggcgagatg
     1681 gtcgcattcc cggacacgct ggtcggcacc gactcgcaca cgacgatgat cagcggcctt
     1741 ggcgtgcttg gctggggcgt cggcgggatc gaggcggagg cggtgctgct cggtcagccg
     1801 ctttaccagc tcatgccgga ggtcgtgggt gtcaagctct cgggccaact ggaaccgggc
     1861 gcaacggcaa ccgacctcgt tctccgcgtg accgagatgc tgcgcagctt cggcgtcgtc
     1921 ggccgcttcg tcgagttctt tggcgcgggc ctctccagcc tgccactcgc cgaccgcgcc
     1981 acaatcgcta acatgtcgcc cgaatacggc gccacgaccg gcttcttccc ggtcgacgac
     2041 gtgacgcttg gatacttgcg caagaccggc cgctccacgg agcaggttga tctcgtcgag
     2101 cggtactgca agcagcaggg cctgttccgg accgacgcga cgccggatcc ggtctacaac
     2161 gcggtactgg agctcgatct tggctcgatc cggccgagtg tcgccggtcc gcgtcggccg
     2221 caggaccgca tcgagctcga caatgtgaag tccaccttcc gggccgccta cgccgatcgc
     2281 ctgccgagcg caaatggcga agaggccccc ttgcccggcg tgccgatcaa ggtgggcgag
     2341 tcgatcgcgc acgttcgcca tggctcggtc gtgatcgcgg cgatcacgag ctgcaccaac
     2401 acgtcgaacc cgtcggtcat gctgggggcc ggcctgctcg ccaagaaggc agtcgagcgc
     2461 ggtctcgacg tcgcgccgta tgtcaagacg tcgctcgcgc cgggctcgca ggtcgtcacc
     2521 cgctacctgg atcagtccgg tttgaccggc tacctggagg cgctcggctt ccatacggtc
     2581 ggctacggct gcacgacctg catcggcaac tcggggccat tgcctggcgc ggtagccgag
     2641 gcggtcgacg acaacgagct ggtcgtcgcg gccgtactct ccggtaaccg gaacttcgag
     2701 gggcgtgtcc accagcaggt ccgggcctcg ttcctggcct cgcctccgct cgtggtggcc
     2761 tatgcgctgg ccggtacggt cgatatcgac cttgccaggg atccacttgg aatcgatccg
     2821 aacagccagc cggtctacct ggccgatatc tggcccaccc gcgacgaggt cgaggcggtc
     2881 gtggccgaga atgtgacctc ggagatgttc caggaggaat acgccaccgt cttcgatggc
     2941 gacgaccgct ggaagacgct gccggtgcca actggcgagc tctatcactg ggatccggaa
     3001 tcgacctacg tgcaggagcc gccctacttc atcgacatgt cgccagagcc gcagccagtc
     3061 cacgatatcg cgggcgcgcg cgtcctcgtg ctggtcgggg attcggttac gaccgaccac
     3121 atctcgccgg ccggtgcgat cgcgcggacc agcccggccg cgcagtacct ggtcaacaac
     3181 gacgtcgagc cgagggactt caactcatac ggatcgcgcc gcggcaacca tgaggtaatg
     3241 gtgcgcggca ccttcgccaa catccggctc cggaacctgc tcgtgccggg caaggaaggc
     3301 aacctgacgg tctacctgcc aaccggcgag gagatgagca tctacgacgc ctcgtacctc
     3361 tatcagcgcg agaacatccc gctcctggcc atcgccggca aggaatacgg ttccggttca
     3421 tcgcgcgact gggcggccaa gggtccgatg ctgctcggga tcaaggcgat catcgccgag
     3481 agttacgagc gcattcaccg cagcaacctg atcgggatgg gcgttctgcc gctccaattc
     3541 aagcccgggg agaacctgag cacgttcggg ctgaacggca ccgagacctt cgcgatctcc
     3601 gggattgccg acaacctgac accacgcaag gtgatgacgg tgaccgcgac caagtctgac
     3661 ggcagcaccg tctcgttcga ggcagtcgcc cggatcgaca gcaaggtgga ggtcggctac
     3721 tacaaaaacg gcggcatcct gcagaccgtt ctccgccgcc tgatggcgag ctaggccggc
     3781 gtggccgacg tccaactgcc gcgatacgca gactatgtga tcgtcggcgc tggaccgggc
     3841 ggatgtgcgg ccgcggccga actcgcccgg tccggttctg cttcggtgct tgtcctcgaa
     3901 gccgggccgg attacgggcc gttcaattcc gggcgctggc caaatgatct actcgaggcg
     3961 ttcgatctcg ccgaaaccca cggctgggga tacgacagcg aatcgacgtt tggaagccgg
     4021 gtcgtcggtt tctcgcgtgc caaggtgatc ggcggctgct cttcgcacaa cggctgcgcg
     4081 gcgatctggg gtcatcgact cgattacgac ggctgggcgg agatgggcct cgacggctgg
     4141 tcgactgacg agttgttgcc cttctttcga gccgccaacg aggcaatgcg agtcgcgatt
     4201 ccgggaccgt cgcacgtcac gccattccat cagttgatgc tcgagtcggc tgccgccgcg
     4261 gaaatcccgc tggtggctga cctgaacgat cttgatgagc cgatcgggat ggcgcccagc
     4321 ccggccaaca tctggaacgg catccgctgg aacgccgcct tcgccttcct cgacccaatc
     4381 cgcgatttgc ccaccgtgac gatcgccggc gacgtcctcg tcgacacggt gcggatcgaa
     4441 aacagccggg cgaccggtgt tcacgtcatc cacgacggag cgagccacat catcgaagct
     4501 ggtacggtgg tgctctcc
//
LOCUS       DATAOZ010000332         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2243028, whole genome shotgun sequence.
ACCESSION   DATAOZ010000332 DATAOZ010000000
VERSION     DATAOZ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243028"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VHR64_08550"
     CDS             complement(<1..534)
                     /locus_tag="VHR64_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302915.1"
                     /translation="MIAALETNGLGKRYGRRWALQDCNLSIPRGRVVGLVGPNGAGKT
                     TLLHLAVGMLAPTAGTISVLGNRPADGPRQLARVGFVAQDTPVYARLTVAQHLQMAAW
                     LNPNWDAELAESRIGRLDLDLRQRAGSLSGGQRAQLALTLAIAKGPELLILDEPVASL
                     DPLARREFLQDLMEVVAV"
     gene            complement(531..923)
                     /locus_tag="VHR64_08555"
     CDS             complement(531..923)
                     /locus_tag="VHR64_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3302916.1"
                     /translation="MIEFQLDSGSGVATYLQLVQQVHQALQLGLLEPGDQLPTAQQVV
                     AKLAINPNTVLKAYRDLEREGLVRARPGLGTFVVASLPRTDPAAQSRFLASMTSWLNK
                     ARAAGLGPDDIEAIYRTAFRNCFAEGVA"
     gene            complement(975..1361)
                     /locus_tag="VHR64_08560"
     CDS             complement(975..1361)
                     /locus_tag="VHR64_08560"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX3302917.1"
                     /translation="MATIDDAAQMALELPDVTEGDNRGNRTWSVAGKAFAWERPFSKA
                     DIKRFGDTTPPAGPIVAVRVVDLEEKEAVIATHPRAFFTIPHFDGYSAVLIQLKAVTK
                     KALREAIVDGWLSCAPPALAEEYLHH"
     gene            1365..1550
                     /locus_tag="VHR64_08565"
     CDS             1365..1550
                     /locus_tag="VHR64_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302918.1"
                     /translation="MTVLLRREMDEIASAFSSAALPAGFHQAAAEVFSRLRLEEAEAS
                     LATLDTVIAALTKKLAI"
     gene            complement(1552..>2019)
                     /locus_tag="VHR64_08570"
     CDS             complement(1552..>2019)
                     /locus_tag="VHR64_08570"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX3302919.1"
                     /translation="KDRRAREESGELRAGPHSISPVSAMSYRADPRVDAYIDALPPWQ
                     QDICRQVRKLVHDADPQVEETIKRTVQPYFVLDGNICALLAARDHVNVFLYDGGMVPD
                     PDGIITGGHDNKTGRTVAIKEGERINARALRTMFKQIIANNRAGGWRKLKSER"
BASE COUNT          378 a          631 c          625 g          385 t
ORIGIN      
        1 cactgcgaca acttccatga ggtcctgaag aaattcacga cgggcaaggg ggtccaggct
       61 agcgacgggc tcgtccagga tcaatagctc cggccctttc gcaattgcca gagtgagggc
      121 gagttgcgct cgctgaccac cagacagcga ccccgcccgc tggcggagat cgaggtccag
      181 gcgtccgatg cggctctccg cgagctcggc gtcccaattg ggattgagcc atgcagccat
      241 ctggagatgc tgagcaaccg ttaagcgcgc gtatacgggc gtgtcctggg ctacgaaacc
      301 aaccctcgcg agctgtctcg gaccgtcggc aggacgattg ccgagcaccg agatcgtgcc
      361 cgccgtcggc gcgagcatcc ccaccgctag atgcaacaac gtagtcttgc ccgctccatt
      421 cggaccgacg agcccaacga ctcttccacg ggggatggac aggttgcagt cttgcagtgc
      481 ccagcgtcgc ccatagcgct tgccaagccc gttggtttca agagccgcga tcacgccact
      541 ccctccgcga aacagttacg gaacgccgtc cggtagatcg cctctatgtc gtccggcccg
      601 agaccggcgg cgcgtgcctt attcagccag ctggtcatgg aagcgaggaa ccgagactgt
      661 gcggcagggt cggtgcgcgg aagcgaggcc accacgaacg tccctagacc cggcctagct
      721 cgcaccaagc cttcccgctc gagatcgcgg taggccttga gaaccgtgtt cggattgatg
      781 gctagcttgg cgaccacctg ctgggccgtc ggcagctggt cgccgggctc gaggagaccc
      841 agctgcaggg cctgatggac ctgctgaacg agctgcagat aggtagcgac acccgagccg
      901 ctgtcgagtt gaaactcgat cattcttcta tcctaccact aagcagttag tggaagcgta
      961 catcagtcgg gaggttagtg gtgcaggtac tcctccgcca gcgcgggcgg cgcgcaggac
     1021 agccagccat cgacgatcgc ttctcgcagc gccttctttg tgaccgcctt caactggatc
     1081 aagactgccg aatacccatc gaagtgtgga atggtgaaga aagccctggg gtgagtggcg
     1141 atcacggcct ctttctcttc aaggtccacc acacggaccg cgacgatggg ccccgctggc
     1201 ggggtcgtgt cgccaaaccg cttgatgtct gccttgctga aaggccgctc ccaagcgaat
     1261 gccttgccgg cgacggacca ggtgcggttg ccgcggttat cgccttcggt aacgtcgggc
     1321 agctccagcg ccatctgcgc ggcgtcgtcg atggttgcca tcctgtgacc gtactgctcc
     1381 gccgtgagat ggacgagatc gcgtctgcct tcagctccgc cgcgttacct gcaggcttcc
     1441 atcaagcagc cgcagaggtc ttcagccggc tgcgactgga agaagcggag gcgagcctgg
     1501 caaccctcga caccgttatc gccgcgttga caaagaagct tgctatctga gtcagcgttc
     1561 gctcttgagc ttgcgccagc cgccggcgcg gttgttggcg atgatctgct tgaacatagt
     1621 ccgcaatgcg cgggcgttga tcctctcacc ttccttgatc gccaccgtgc gtcccgtttt
     1681 gttgtcgtgg ccaccagtga tgatgccgtc gggatcaggg accatgcccc cgtcatagag
     1741 gaacacattg acgtggtcac gcgcagccag aagcgcacag atgtttccgt cgagcacgaa
     1801 ataaggctga acggtgcgct tgatcgtttc ctcgacttgg ggatcggcat catggaccag
     1861 tttgcggacc tgacggcaga tgtcctgctg ccagggaggc agagcatcga tgtaagcatc
     1921 gacccgcggg tcggcacgat agctcattgc ggagactggg gagatcgagt gaggcccagc
     1981 cctcagctca ccgctttctt cacgagcgcg gcgatcttt
//
LOCUS       DATAOZ010000333         5241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6974980, whole genome shotgun sequence.
ACCESSION   DATAOZ010000333 DATAOZ010000000
VERSION     DATAOZ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6974980"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VHR64_08575"
     CDS             <1..632
                     /locus_tag="VHR64_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX3302920.1"
                     /translation="ANWRLDVARVAAVRAALGPKIGIKIDVNQGWMTPGIAIPAIRQI
                     LASNPDYIEQPVDQHDIAALAEVRRATGAPIMADEAVHGVREMQRVVELRAADLVNIK
                     LMKTGGLVNAMRVNAIAEAAGIAGQVGTMVESSIASAAGLHFAMAHANIKTVEMGGPL
                     MIADDIGNLRQYYNHNRITLSDAPGLGVELDVDAIESSTWHKITVPAQA"
     gene            complement(636..1082)
                     /locus_tag="VHR64_08580"
     CDS             complement(636..1082)
                     /locus_tag="VHR64_08580"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX3302921.1"
                     /translation="MPEPKIPSTSSQSTQSASTERAEQEDSGTSQKKKVSTSTVSNRY
                     EVDLTRHAEKDLKALRAWNAAIFRHLARLETNPEAGHLLKGALHGCRSLELNLKGSGA
                     YRAVYVIAPGERVCTVFLIGPHENIYREAERRVQALGKSGEIPDPD"
     gene            1478..2866
                     /locus_tag="VHR64_08585"
     CDS             1478..2866
                     /locus_tag="VHR64_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3302922.1"
                     /translation="MIIDRIFTPGLAQVAYLIADESSGDVAVIDPRRDVDAYIDWADV
                     RGYRITAILETHVHADFVSGSPELAARTGAPVYASWESEQTSPHTPLADAESVPVGSL
                     SLKALWTPGHTPEHLAFLLFDPAQGTQPVALFSGDVLFVGEVGRPDLLGADQIEELAT
                     KLYHTVMDRLFRLPDDLIVYPGHTAGSACGKKIGEAPYTTIRGERIGNYAFQARSRDS
                     FVQMVLDGMPLAPTYYPVLKQVNKVGATPVANLPEPQALDPAEVAAAAEDGAVIIDAR
                     PFEQFGEGHIPGAVAAGLGENFVAWMGWLAPYDRDIVLVLSSDNELDEAVTELHRIGL
                     DRIAGYLADGMRSWSSDGRDTRTLPQISAQELAHHREVRPNLIVLDVRSDEEWRDEHI
                     PNGIHLYAGKIAQGADPNLPKDAEIAIICGSGYRSSFVASLLMERGYRHLINVDGGMD
                     DWNKRKLPITRG"
     gene            2906..3247
                     /locus_tag="VHR64_08590"
     CDS             2906..3247
                     /locus_tag="VHR64_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3302923.1"
                     /translation="MSLRTMAGATDATPEVDIAETARVWADRSAQLVDVREPDEWDEA
                     HIPGSIHIPLGELGRRSDELIKEIPVVVICRSGQRSLAGTDELLARGFADVASLNGGI
                     LAWARAGHPVE"
     gene            complement(3618..4550)
                     /locus_tag="VHR64_08595"
     CDS             complement(3618..4550)
                     /locus_tag="VHR64_08595"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX3302924.1"
                     /translation="MNQSIPRRPFGKSGEEVSLLALGGAHIGLSSLSEDESIRLQHAA
                     FDAGVTFLDNAWEYNAGISEVRMGNAIKSWRSRDDLFVMTKDCAHDRKAGNSLVKLEQ
                     SLRRLQCDHLDLWQLHEVVWEDDPDWIFAPGGSAEALLKAKEQGKVRYIGFTGHKSPA
                     IHKRMLSQGFPWDAVQMPLNVLDAHWESFGKEIVPICQEQGIAIIAMKSFADGHLFKA
                     GSGVTPEEALRYTMSLPVATVVSGMESMEILNQNVEIARNFTPLTDAERETIIAKTAS
                     LADGGQFEPFKTTRDYEAEEGRHAHNYPQRFAAD"
     gene            complement(4968..>5241)
                     /locus_tag="VHR64_08600"
     CDS             complement(4968..>5241)
                     /locus_tag="VHR64_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEX3302925.1"
                     /translation="MNIYLGKHFEEFIRKQVESGRYANASEVVREALRLYEDNEIKLE
                     RLRAKIAEGMASIERGNVIEIDDLDAYFDDLIRRVTERVEQQRKSA"
BASE COUNT          968 a         1695 c         1564 g         1014 t
ORIGIN      
        1 cagccaactg gcggctcgat gtcgcccggg tcgcggccgt gcgcgcggcg ctcggcccga
       61 agatcgggat caagatcgac gtcaaccaag gctggatgac acccggcatc gccatcccgg
      121 cgatccgcca gatccttgcc agcaacccgg attacatcga gcagccggtc gaccagcacg
      181 acattgccgc cctggccgag gtccggagag cgaccggcgc gccgatcatg gccgacgagg
      241 cagttcacgg tgtgcgggag atgcagcggg ttgtcgagct acgggcggcc gacctggtca
      301 acatcaaact gatgaagacc ggcgggctgg tcaacgcgat gcgggtgaat gcgatcgccg
      361 aggcggccgg gatcgccggt caggtcggga cgatggtcga aagctcgatc gcctcggccg
      421 ccggactcca cttcgccatg gcccacgcca acatcaagac ggtcgagatg ggcggccccc
      481 tcatgatcgc cgacgacatc ggcaatctcc gccagtacta caaccacaat cgcatcactt
      541 tgtcggatgc tccggggctc ggcgtcgagt tggatgtcga cgcaatcgaa tcaagtacat
      601 ggcacaaaat cacagtcccg gcccaggctt gagcgctagt caggatctgg gatttcgcct
      661 gatttgccga gcgcctggac tcgccgttcc gcctcccggt agatgttctc gtgaggtcct
      721 atgaggaata cggtgcatac tcgctcaccc ggggcgatga catacacggc gcggtaagcg
      781 ccgcttccct ttagattgag ttcgagtgag cggcagccat ggagggcgcc tttgaggagg
      841 tgacccgcct caggatttgt ctcgagccgg gcgaggtgac gaaagatggc ggcattccaa
      901 gcgcggaggg ctttcagatc cttttcggca tggcgcgtaa ggtcgacctc gtagcgatta
      961 ctcaccgtcg aggtcgatac cttcttcttt tgcgatgtcc cggagtcctc ttgttcggcc
     1021 cgctcggtgc tcgcggattg cgtgctctgc gaggaggtcg aagggatttt cggctcgggc
     1081 atagcggaga actcctgagt cgccctccac ttgaacgaaa aagatgtctc cagcttcgat
     1141 ccccagttgc ttacggatag catctggaat aagaagccgg cccttctcgt ctaccgtgga
     1201 gtgcctaaca cttttcccat gacacagctc tccctcgaat aggtacagtt cagaaggacc
     1261 attccatctg agtgggcaaa tcccactata ggatcatacg gctatgcacc tgtcgcgtca
     1321 accaaccact aggatagggg ccaaagctga ccgccgtggg tgtaggtcgt gcctgcttga
     1381 catcggtggt ggccgtacta cgcttgcctt gttcccgtgc gctcgttgct gagcgctgtt
     1441 tacttgcgtt cgggccagtg tcgccccgag ggttgccgtg atcatcgatc gcatcttcac
     1501 ccccggcctc gcccaggtcg cgtacctcat cgccgatgag tcgtccggtg atgtcgccgt
     1561 gatcgatccg cgccgggatg tcgacgccta catcgattgg gccgacgtgc gcggctaccg
     1621 gatcaccgcc atcctcgaga cgcacgtcca cgccgatttc gtctccggat cacccgagct
     1681 ggccgcccgc accggcgccc ccgtctacgc gagctgggag agcgaacaga cttccccgca
     1741 cacgccgctt gccgacgccg aatcggtccc ggtcggctcg ctctcgctca aggctctttg
     1801 gacgcccggc cacacgccgg aacacctcgc ctttctgctg ttcgacccgg cccagggcac
     1861 gcaaccggtc gcgctcttct cgggcgacgt cctcttcgtc ggcgaggtcg gcaggcccga
     1921 tctgctcggc gcagaccaga tcgaggagct cgccacgaag ctctaccaca cagtcatgga
     1981 ccggctcttc cgcctgcccg atgacctgat cgtctacccc ggccacacgg cgggctcggc
     2041 ctgcggcaag aagatcggcg aggcgcccta caccaccatc cgtggcgagc ggatcggtaa
     2101 ctacgccttc caggcccgct cccgcgattc gttcgtccag atggtgctcg atggtatgcc
     2161 acttgccccg acctactacc cggtcctgaa gcaggtcaac aaggtcggcg ccactccggt
     2221 agccaatttg cccgaaccgc aggcactcga tcctgcggag gtggccgccg cggccgagga
     2281 cggcgcagtg atcatcgacg cgcgaccgtt cgagcagttt ggggaagggc acatccccgg
     2341 cgcggtcgcg gccggcctgg gcgaaaactt cgtcgcctgg atgggctggc ttgctccgta
     2401 cgaccgggac atcgtcctcg tgctgtcgtc cgacaatgag ctcgacgaag cggtcaccga
     2461 gctccatcgc atcgggctgg accggattgc cggatacctc gccgacggga tgcggtcgtg
     2521 gtcttcagac ggccgtgaca cccgtaccct tccgcaaatc agcgcgcagg agctcgccca
     2581 tcaccgcgag gtgcggccaa acctgatcgt gctcgatgtc cgcagcgacg aggaatggcg
     2641 ggacgagcat attcccaacg gtattcatct ctacgccggc aagatcgccc agggcgcgga
     2701 tcctaacctg cccaaggatg ccgaaatcgc catcatctgc ggcagtgggt accgctccag
     2761 cttcgtcgca agcctgctaa tggagcgtgg atatcggcat ctgatcaacg tcgatggtgg
     2821 aatggacgac tggaacaagc gcaaactacc tatcacgcgc ggatagccgt cactcccgcg
     2881 caacctgatc caacgaggag attcgatgag tctgagaacg atggcgggcg cgacagatgc
     2941 gacgcccgag gtcgatattg ccgaaaccgc ccgcgtctgg gccgatcgct ctgcccaatt
     3001 agtcgatgtc cgcgagccgg acgaatggga cgaagcgcac attcccggct caatccacat
     3061 cccgctcggc gagcttggtc gccgttccga tgagctgatc aaggagattc cggtggtcgt
     3121 catctgccgc tccggtcagc gcagcttggc ggggacggat gaactcctcg cccgtggctt
     3181 tgccgatgtc gcgagcctca acggcggcat cctcgcctgg gcccgcgccg gccacccggt
     3241 ggaatagcgg gttgagggtg gcgagttgag ggttgaggga acgaaaaacg gaagacccat
     3301 gatgactttc gcgcccatat tatcggtgac atcaacatga cagatccgcg gctgagtcat
     3361 gacccagagg ccaacgcact ctagctgcgt ctctctgaga acgacgtagc agaaacgctg
     3421 gagctttcac gctccgtcta tctcgacgtt gatgctgaag gcgcaccggt cggcttcgat
     3481 atcctcaatg ccgatccgag cctgcttgcg ggactcccgc acatgcccga gggcacgtca
     3541 ctcgtcgaga tcttgagacg ccacgcggcc tgacaccctg ctgctcgcct gctcgacttc
     3601 tcgtctactc gccttgatca atctgcggcg aaccgctgcg gatagttgtg agcgtggcgg
     3661 ccttcctcgg cttcgtagtc gcgggtggtc ttgaacggct cgaattggcc gccgtcggcg
     3721 agtgacgccg tcttggcgat gatcgtctcc cgctcggcat cggtcagcgg cgtgaagttc
     3781 cgggcaatct cgacgttctg gttcaggatc tccatcgatt ccatgcccga gaccactgtc
     3841 gccaccggca ggctcatcgt gtagcggagc gcctcctcag gcgtcacgcc actgccggcc
     3901 ttgaagagat gcccatcggc gaagctcttc atcgcgatga tcgcgatccc ctgctcctgg
     3961 cagatcggga cgatctcctt gccgaagctc tcccagtgcg catcgagcac gttcagcggc
     4021 atctggaccg catcccacgg gaagccctgc gagagcatcc gcttgtggat cgccggcgac
     4081 ttgtgcccgg tgaagccgat gtagcggacc ttgccctgct ccttggcctt gagtagcgcc
     4141 tcagccgaac cgcccggcgc gaagatccag tcgggatcgt cctcccagac gacctcgtgg
     4201 agctgccaga gatcgaggtg gtcacactgc agacggcgca ggctctgctc cagcttgacc
     4261 aacgagttgc cggccttgcg gtcgtgggcg cagtccttgg tcatcacgaa gaggtcgtcg
     4321 cgggagcgcc aagacttgat cgcattgccc atgcgtactt ctgagatgcc ggcgttgtac
     4381 tcccacgcgt tgtcgaggaa ggtcacgccc gcatcgaatg ccgcatgctg cagacgaatg
     4441 ctttcgtctt cgctcagcga ggaaagccca atgtgcgccc caccgagcgc caacaggctg
     4501 acctcctcgc cggacttgcc aaacggtcgt cggggaatgg attgattcat gcgcaactcc
     4561 agtctcaagt ggccaattgc cacgagccag ctattggacc gtaccttttc accccggaac
     4621 ttggtcgggc cttagttacg gatgcccaga actattccgg gtcaaccgca ccgagttccg
     4681 cttcagcgtc ctcacgctcg tggagcaccc gaactatgta gatgtgatcc tcgcgcacca
     4741 aatagaagat gcggtgctgt tgaactgcgg cacaccgcat ccccggaaac agatccgagc
     4801 ggtcgtgtcc cgagtgagga tactcacgca ggcgccggaa gcctacccgc agaccgtcca
     4861 ggtatcgcct ggcctgcgac atcccccaag acaggcctga atgttcaaga atgtctgcaa
     4921 gatcttcgtc agtctcccgt gaagtagcta atcgaaggag cggcatatta cgcgctcttc
     4981 ctttgctgct ccacacgctc agtcactcgg cgaatcagat cgtcgaaata ggcgtcaaga
     5041 tcgtcgatct cgatcacatt gccgcgctcg atggaagcca tgccttcagc gatctttgcg
     5101 cgcaaccgtt ccaacttgat ctcattgtct tcgtacaggc gcaatgcctc gcggacgact
     5161 tcgctggcgt tggcgtagcg gcccgactcg acctgcttgc ggatgaactc ttcgaaatgc
     5221 ttgcccaggt agatgttcat g
//
LOCUS       DATAOZ010000334         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4873504, whole genome shotgun sequence.
ACCESSION   DATAOZ010000334 DATAOZ010000000
VERSION     DATAOZ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4873504"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1729)
                     /locus_tag="VHR64_08605"
     CDS             complement(56..1729)
                     /locus_tag="VHR64_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302926.1"
                     /translation="MERAATVGRRGIGFGEVFRAVDFTDRQIAGLFVVLAVITVIPVL
                     LYPWPPLADYINHLSRMHIIATMGSDPDLSKFYDVNWQIIPNLMMDMIVPPLTRLMSV
                     YAAGQVYTIVSFVLIISGTLALNRQLFGRWSVLPLIAFPLLYNNVFLVGTMNYIFGMG
                     LALWAMTAWVRLREHNPVIRLTVSLLFVLALFFCHLFAVGVYGLGLAAFETSRLVEHY
                     RSRGNRADVSSAKLWPLVDFVATGLPFLPVLPLLMMSPTWGLRASFVWELNGKLDGLL
                     YVIEVYSHFAALLLTGIVAFAAGFGLRHRALQFHSTGWALLIIGSITYFAMPRIIFET
                     YMADQRLPISLAFMVIACAHLNLGHDYVRRGFATVLVLLLAIRVFEVQTVWSDASKTI
                     VAFRDSVRLIDRGSKILVAYADPDAGDDTKDLVLVHAACSAIIERSALVTTAFTVKGK
                     QILEVREPYRDRVDRLDGTPPSVAQLLQVAEEGPQQDGADNYWNKWTTDYDYVYVLFT
                     DSSGYENPDPTRLTALYTGERFALFRINPVDTQPGPKVADGRTDLLPAR"
BASE COUNT          421 a          558 c          591 g          298 t
ORIGIN      
        1 ggcaagacct ggattcacaa gacctggatt cacaagtctg gatttacaat ccggatcagc
       61 gggctggcag caaatccgtg cgaccgtcag cgactttcgg ccctggctga gtatcgaccg
      121 ggttgatccg gaacagggca aaacgctcgc cggtatagag tgcggtcagg cgcgtcggat
      181 cgggattctc atagccgctg ctgtcggtga acagtacgta gacatagtcg tagtcggtgg
      241 tccacttgtt ccagtagtta tcggcgccgt cctgctgcgg accctcctcg gcgacctgca
      301 ggagctgcgc caccgagggc ggcgtcccgt cgagccggtc aacgcggtcg cgatagggct
      361 cgcgcacctc caggatctgc ttgcccttaa cagtgaaggc ggtcgtcacc agcgccgagc
      421 gttcgatgat ggcagagcag gccgcgtgca cgaggacgag gtctttcgtg tcgtcgcccg
      481 catccgggtc ggcgtaggca acgaggatct tggagccacg atcgatcaga cgcacggagt
      541 cgcggaaggc gacgatcgtc ttagaggcat cggaccagac ggtctgcacc tcgaacaccc
      601 tgatcgccag cagcagcacc agcacggtcg cgaaaccgcg gcgcacatag tcatgcccga
      661 gattgagatg cgcgcaggcg attaccatga aggcgagcga gatcggcagg cgctgatccg
      721 ccatgtaggt ctcgaagatg atccgcggca tggcgaaata ggtgatggag ccgatgatca
      781 gcagagccca gccggtcgaa tggaactgca atgcccgatg gcgcaggcca aagccggccg
      841 caaaggccac gatccctgta agcagcaagg cagcgaaatg ggagtagacc tcgatgacat
      901 agagcaaacc atcgagcttg ccgttgagct cccacacgaa cgaggcgcgc agaccccagg
      961 tcgggctcat catcaggagc ggcaacaccg gcaggaacgg aagcccggtg gcgacgaaat
     1021 cgacgagcgg ccacagcttc gcgctcgaaa cgtccgcccg attgccgcgg ctcctgtaat
     1081 gctcgaccag gcggctggtc tcgaaggcgg cgagcccgag tccataaacc ccgaccgcga
     1141 acaagtggca gaagaaaagc gccagtacaa acagcagcga gactgtgagc cggatgaccg
     1201 gattgtgctc gcgcagccgg acccaggccg tcatggccca gagtgcgagg cccatcccga
     1261 agatgtagtt catggttccg acaaggaaca cgttgttgta gagcaacgga aacgcgatca
     1321 gcggcagaac cgaccagcgt ccgaacagct ggcgattgag cgcaagcgtg cccgagatga
     1381 tgagcacgaa gctcacgatg gtgtagacct gcccggcggc ataaacgctc atcagccgcg
     1441 tcagcggcgg aacgatcatg tccatcatca ggttgggaat gatctgccag ttgacgtcat
     1501 agaactttga cagatcaggg tcggagccca tcgtcgcgat aatatgcatg cgcgacaggt
     1561 ggttgatgta atcagcgagt ggtggccacg gatagagcag gaccgggatg accgtaatca
     1621 ccgcgagcac cacgaacagg ccggcgattt gcctatcagt gaaatcaacc gcgcgaaaga
     1681 cctcgccgaa gccgatcccc cgccgtccta ccgtagccgc tcgctccacg cttgcggtcc
     1741 gcacgcccac cggttcgctg atgtcaacca ttctgccaac aacagattgt gactcgctgc
     1801 ccggcgggat cgagcgagcg ggccgtgaag tgaattgctt gcgcccccgc gggtcccgtg
     1861 tgcctgaa
//
LOCUS       DATAOZ010000335         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8820456, whole genome shotgun sequence.
ACCESSION   DATAOZ010000335 DATAOZ010000000
VERSION     DATAOZ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8820456"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..666
                     /locus_tag="VHR64_08610"
     CDS             175..666
                     /locus_tag="VHR64_08610"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX3302927.1"
                     /translation="MQPTPSEPTRSPLGALSSDAITGHCLCGAVTITVAGEHDPRVGA
                     CHCRMCQRWSGGLFLCFTAAAAAVTVQGEVVRYRSSAFAERAFCPRCGSHLWFNDVQE
                     GGEPRSYELMPGLFDAARGWPLRSEIYVDRAMACVHLAGDHRRKTRAEYEAENPFVEG
                     DME"
     gene            complement(736..2022)
                     /locus_tag="VHR64_08615"
     CDS             complement(736..2022)
                     /locus_tag="VHR64_08615"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3302928.1"
                     /translation="MITRDLVIVGGGIAGVICLKYARDAGFDVELLEQADGVGGLWRK
                     LPAWQDIQIRRQDWTLGDIPIAGESQPDIVANIEAWVERYGLARYIRLSAPVRSAAWR
                     SDGWHVATDADVTRARCLVAASGGHSRPHVPHVLRSDSRVDEFHSSELRDPGLLSGRR
                     IVVVGGGASAYDLLDLCLERGARSVVWIYRSLKWMRPTLSPKVLGTNMRLLARHQMIR
                     RSTSALNKHLDRDLRARYERFGLQDIVPDESFDIGRHQLIPGRHRMIRQFDRIERHQA
                     EIVHVAGRRIGLSTGSEVEADTILWATGYDMDLSYLAVDALRSETSIERVAARCGSVC
                     RSLDASNLYIMGPGVLETSGSTPWAYAHLAKSIMSDIAGSRALASDAVAKRLNYFDLA
                     RHLARADRRNYPPLLWVLRYLWLAVLHPYERPMPVP"
     gene            2170..>2445
                     /locus_tag="VHR64_08620"
     CDS             2170..>2445
                     /locus_tag="VHR64_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3302929.1"
                     /translation="MDALSELLRALRLKGAVFIDAELSAPWAIRTPSPRSIANVMMPD
                     AQRIIPYHFVIEGKCWIRLPGEEAIPVRAGEVALFPHGDVHDLFSGPD"
BASE COUNT          417 a          829 c          774 g          425 t
ORIGIN      
        1 atcctacgct ctgtcaggct cgaaccgttt cgccttgaag actcgagtcg aagccaatat
       61 cgccaggctt ggcaccacgc agtatcttgt ccacctgtct cgcgcctcgg cgctgcccgg
      121 cgaggccggc ccaccgcttg ctcctgcccg acgtcgactt ccggagccac ccacatgcag
      181 ccaaccccat ccgagcccac ccgctctccc ctcggcgctc tctccagtga tgcgatcacc
      241 ggccactgcc tgtgcggcgc ggtgacgatc accgtcgcgg gagaacatga tccgcgcgtc
      301 ggcgcctgtc attgccggat gtgccagcga tggtcgggcg gactgttcct gtgcttcacg
      361 gccgccgcgg ccgccgtgac ggtccagggc gaggtcgtgc ggtaccgctc ctctgccttt
      421 gccgagcggg ccttctgccc gcgctgcggt tcgcacctgt ggttcaacga tgtccaggag
      481 ggtggcgagc ctcgcagcta cgagctcatg cccggcctct tcgacgcggc tcggggctgg
      541 ccgcttcgat ccgagatcta cgtggaccgt gcaatggcgt gcgtccacct cgccggcgac
      601 catcgccgca agactcgcgc cgagtacgag gcggagaacc cgttcgtcga gggagacatg
      661 gaatgacacc cggcgggtgc cttgccgctg gccgctgtcg cgcctgatcg aacctgaacg
      721 gccgcgatct tcgattcatg gtaccggcat tggccgctcg tacggatgca gcaccgcaag
      781 ccacaagtac cgaagtaccc agagcaacgg tggatagttg cggcggtcgg ctcgcgcgag
      841 atgacgcgcg aggtcgaagt agttgagacg cttcgcgacc gcatccgagg cgagagcgcg
      901 cgatcccgcg atgtcggaca tgatggactt cgccaaatgg gcgtatgccc agggcgtcga
      961 cccgctggtc tcgagcactc ccgggcccat gatgtagagg ttggatgcgt cgagcgagcg
     1021 gcagaccgag ccgcatcgag cagccacccg ttcgatgctg gtctcgctcc gcagcgcgtc
     1081 gacggcgagg tagctcaggt ccatgtcgta cccggtcgcc caaaggatcg tgtcggcctc
     1141 gacctcggag cctgtcgaca agcctattcg ccgccccgcg acgtgaacga tctcggcttg
     1201 atgccgctcg atccgatcaa actgtcgaat catccggtgc ctgccgggga tcaactgatg
     1261 tcggccgatg tcgaacgatt cgtcgggaac gatgtcctgc aatccaaacc gctcgtagcg
     1321 tgcgcgcaag tcgcggtcca ggtgcttgtt gagcgctgac gtggagcggc gaatcatctg
     1381 atgccgggcc aacagccgca tgttggtgcc gaggaccttc ggactcagcg tcgggcgcat
     1441 ccatttcagc gatcggtaga tccagacgac gctccgcgcg ccgcgctcca ggcacagatc
     1501 caacaggtcg taggccgacg cgccaccacc gacgaccacg atgcggcgac cgctcagcag
     1561 gcccgggtct ctcaactccg atgagtggaa ctcatccacg cgtgagtcgg agcgcagaac
     1621 atggggcaca tgggggcggc tgtgaccacc gctggcagcg accaggcagc gcgcccgagt
     1681 gacgtctgcg tctgtcgcga cgtgccatcc gtcggagcgc catgccgctg aacggacggg
     1741 ggcagacaac cggatgtatc gcgccagccc gtaacgctcg acccaggctt cgatgttggc
     1801 gacgatgtcc ggttgactct ctccagcgat cgggatgtcg cccaaggtcc aatcctgccg
     1861 ccggatctgg atgtcttgcc aagcaggcag cttgcgccac aacccgccga cgccgtctgc
     1921 ctgctcgagc agttcgacat cgaatccggc atcccgggcg tacttgaggc agatgactcc
     1981 cgcaatgccc ccgccgacga tgacgagatc ccgtgtgatc atgctgctcg acgcttcgtg
     2041 tggggaatat catcgtgctg ctggtgcatg gatggcgtcc atgccgggcc gccgccggga
     2101 ttgatcgaaa tgccacagat tgaaggcacg cgctcgacct gacgcgggca gtagctacac
     2161 gccgcatcca tggacgcgct gtcggaattg cttcgcgcgc tgcgcctcaa aggcgccgtg
     2221 ttcatcgacg cggagctgag cgcaccctgg gccatccgga cgccgtcgcc acgctcgatc
     2281 gccaacgtca tgatgccgga cgcgcagcgc atcatcccgt accacttcgt catcgaaggc
     2341 aagtgctgga tccggcttcc aggcgaagag gccatcccgg ttcgcgccgg agaagtcgcc
     2401 ctgtttccgc acggcgatgt ccacgacctc ttcagcgggc cggac
//
LOCUS       DATAOZ010000336        10436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3820670, whole genome shotgun sequence.
ACCESSION   DATAOZ010000336 DATAOZ010000000
VERSION     DATAOZ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10436
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3820670"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VHR64_08625"
     CDS             complement(<1..483)
                     /locus_tag="VHR64_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX3302930.1"
                     /translation="MSRLVSTTDRRLIRLLQQNARVSFAELSRVTGIPESTVRRRVER
                     MQERGIIRFAMIADADQLGYDISAMVGLRIDLSRLHEIGATLAAMPEVVFASFLTGSF
                     DILAQVVVESQDALVELLTRLAGTDGVRSTETFLMPRMLKPLTAWVIPEPEEDDATDR
                     D"
     gene            859..1896
                     /locus_tag="VHR64_08630"
     CDS             859..1896
                     /locus_tag="VHR64_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX3302931.1"
                     /translation="MTVLRKAMAGAFVLGLLVAQLGLSGTAQADSSGVDSYIELSATK
                     PASGCTIDGSFEVRSDGYPVAGVEVFIGLYVGTDLISSDSALTDDNGVTYLSIDPSAA
                     DGADAWIDVTLNDTYWWGTASYPGAGGTCYDGALNANKSGQIAGAPSSTDDASSAPSS
                     NAHVSSDAVIIPEVGFYVQQRNLSCEYAAVYIATAAWGNGISEYATEATVGWSDNPHE
                     GYRGNINGWWGNTYDYGVYAEPLSWALAQYGFVGEVAYTHGDPSWLMNEIAAGNPTIV
                     WLGMWGDQSVYEAGYRVVAGMHVMVAYGYDDGGVYLSDPATGGSRYYDWGTFEWMWGV
                     MDGMSLSVYPA"
     gene            2188..3072
                     /locus_tag="VHR64_08635"
     CDS             2188..3072
                     /locus_tag="VHR64_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3302932.1"
                     /translation="MAGLSRRALIGGALGMAASMTSRIIVGAETAGVPATVGGSTIKL
                     RVRARRDSDVTGSIPSNAPVNVIEGPTPDGWYRVEGETKHGSQRGWTHGDGLVFAQSA
                     VFLWDAGVFSGPTDATGWLATIRHGVVVTVAGPGSNGFTFIRFGGLQGYVAASALQLT
                     DQPATDPLGEWWADVNRSTLTVNLMIGSTIVDSFPAAMSTETGAGFYSTAPGTYWIYE
                     KVAGLQYTPYAKAYFMYWCGFDPNRYNGFHSWTMDGNGYVLNGGWGNTAGCVATEPKN
                     AAVIYNFLSLNSRIEIHW"
     gene            3296..4264
                     /locus_tag="VHR64_08640"
     CDS             3296..4264
                     /locus_tag="VHR64_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3302933.1"
                     /translation="MLHTGVGARGLSWHSLRVNGAFGWRVVLVSVVLFSLFSATIQPS
                     RAAIDSYVDTDVLYLRTDPGTDAGVIAEMYDGDYVAVIDGPTEDGWYFLDYEGSQGWA
                     YGAYLNVGGSPGWESAAADASVGGSGPTVWVATDALNIRAEPTRKSWVLGTVGQGESL
                     SIAGEKDNGFYPVWYGDNIGWVAGKYLSWEPVSGGEERWIDVDRSSSTIRLMVGIESV
                     GYWWAAMGFDDSDNGFYSTAVGTYYVYEKNAALTWTDWARGYIEYWVAFDPDRFNGFH
                     SWTMDKNGNVIEGGDGATGGCVAMEPGLAAALYEFATYGMRVEIHW"
     gene            4399..6432
                     /locus_tag="VHR64_08645"
     CDS             4399..6432
                     /locus_tag="VHR64_08645"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX3302934.1"
                     /translation="MQRRSLFVPRRAVIFLILIAAMLVAGLGVGSPQPAAAKSVTWRN
                     YDVTLTLNGDSTFHVAERQIISFEGGPFHFAYAGLSLAEVEDLTGIKVSEIRNGSTIA
                     YQQNLSGDPETYSLERSSSAVTVTWNFPTTSNQDRTFLVEYDVIGGLRVYDTDAGQRQ
                     QIWWNAVNSDVTDIAPVDSASFNVQLPQPVANLSDVVIDGSDGSRDPAAHTTDGRVYT
                     WTATNLGEGDTLWGRLEFPAMVNATAPAWQKSVDEQRLREQERSSRDALLRLLLAGAA
                     LLTLTAGGVGLFGFWYMRGRDPGVGAVASYLSEPPDDLPPGAAGALIDEEVNERDIVA
                     TLVDLGRRGVLNIKQEEVGSVFKRNEYTISLKQDAKDLRPFEQTFVSSIMSGTEAGKS
                     VTLGDAKERFSRKVGTIRSQMYDELVKRGYFPVSPQTTRTRYQSFASAAIVGLIIIAA
                     IFGGAILDISGWFIVPAIAILVILFSLYQTAKYMPRKTVAGAESAAKWRAFKSYLADL
                     DRQKIGDNAGEIFEKYLPYAVAFGLERTWVTRFAEAGAQAPDWYGGFGGYGGGWFDPQ
                     VGRRSGRRTVWVGGPGWGGMPTSTGAPRTGDGGGVDMPGLPDIQSASDKGAKSLQSMS
                     GGLFDLFDLAGSAFKGFSGSGKSGGFSGGGRSFGGGGGFGGGGGGGGGGRGFG"
     gene            6429..7175
                     /locus_tag="VHR64_08650"
     CDS             6429..7175
                     /locus_tag="VHR64_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX3302935.1"
                     /translation="MTRRRFVLGCPVDVLNPAAFKASLPALASGDCLTDIVTLNPEQI
                     MAARRDPEVARLIRKAQLCTVDGIGLELALRAQGVTDVQRITGVDLVTSVVEQRIPTF
                     LLGGAPGAAEEAGRRLTARFPLAQIAGSWSGGTPRAKDDPQSIARIALSGAKAVAVAY
                     GAPAQTAWIERNRAALEDAGIRIAIGVGGSLDFYAGYVRRAPEWAQRFGLEWLFRLIA
                     EPWRLRRQLVLPKFAVLALIEAGRGRIGHA"
     gene            7226..7780
                     /locus_tag="VHR64_08655"
     CDS             7226..7780
                     /locus_tag="VHR64_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302936.1"
                     /translation="MTVTETPVFTTEDVPCARHPKVQTRLRCSRCGTPICPQCAVRTP
                     VGLRCPDCAGVRGLPTYSTPGASMLKAAGAGVAVGLVFALLFAWIPQWNFYLSLALGF
                     GVAEAMARAAKNKRGADLQVLGIVIALGAMGLGRAILAWREDLTWAQVNAFSPYVENL
                     LRMSLTPDGLFAALTVLIVWYRFR"
     gene            7788..9362
                     /gene="murJ"
                     /locus_tag="VHR64_08660"
     CDS             7788..9362
                     /gene="murJ"
                     /locus_tag="VHR64_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX3302937.1"
                     /translation="MHAPSRSGVARAALLITAGGLASRILGLVREQLAAGYFGAGDKV
                     AAFQIADNVQTLLFDLVISGMLQAALVPVLVEFAGPGNRQILRRISGTIATVAVVLVG
                     LATVIGWLFTPQLVSVMTSLGAGDQSRSPETVAMTEHMVRIVLPGVLFLALGTVFSAV
                     LYSLGRASGPATALAARNLVVVASILLLANRLGVESMAYGVLIGGALVAVIQLPWLMQ
                     HDAVPIPNLHLRDPAVRHIGRLYIPVFLGLMVSTVQVVVDRNLAWRAESDALGAMRYA
                     TTLVQSVLGLVAAAISLAALPVLATHFSQRDEERFDQTLVQAVRMVTVLIVPAVFGLA
                     ALSRPVVGLLFQHGETGPAEAHSIAVVLLAYLPGTLFAAYDQILIYMFYSRRSTWIPV
                     MVGVLSVGAYFVAAALLANRYGAVGLATANSAQFVSHTLVLGFIARKRVLSVWGQFSG
                     LLALTIAGSVACAAVAYLSYRGIAGTTGGIVREIGAVLIPAALGGAVYLAALLAAHVP
                     EIHVIKNRVGARLPAR"
     gene            complement(9325..>10436)
                     /gene="larC"
                     /locus_tag="VHR64_08665"
     CDS             complement(9325..>10436)
                     /gene="larC"
                     /locus_tag="VHR64_08665"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX3302938.1"
                     /translation="TSNGITGTHASVPPEQGHHSRTWSEIRALIQRANLDAVVRDNAV
                     RIFARLAEAEASVHAVPVDKVHFHEVGALDTIVDVVGVVAGFRLLGVERISCGPLRVG
                     GGTVKTAHGLLPVPAPATSRLLAMSTALIATPMPGETEAGELLTPTAAAILGTLASFD
                     RPAMTIQAVGSGFGTKELPWANICRIVIGETEAGPSGGESLVVIETNIDDMNPQFVEI
                     LIERLFAAGALDAWTTAIGMKKGRPAVMISALARPSAADELTALLIQQSTTLGVRQTQ
                     VNRIAADRQMEAVLTRWGSVRVKLKIWHGRVIDLAPEYDDCAAIARAQDVAVRDVWNE
                     AYHLGQPYIGRRVGASGALARLDPIEPEDGLRRGS"
BASE COUNT         1708 a         3191 c         3529 g         2008 t
ORIGIN      
        1 atctcggtcg gtggcgtcgt cctcttccgg ctcggggatg acccacgcag tgagcggctt
       61 gagcatgcgc ggcatcagga acgtctcggt cgaacggacg ccgtcggtgc cggccagccg
      121 ggtcagcagc tcgaccaatg catcctgtga ttcgaccacg acctgcgcga ggatgtcgaa
      181 gctgcccgtc aggaacgacg cgaacacgac ctcaggcatg gcggccaggg tcgccccgat
      241 ctcatgcagc cgggagaggt cgatgcgaag accgaccatg gcgctgatgt cgtagccgag
      301 ctgatcggca tcggcgatca tcgcgaagcg gatgatgcca cgctcctgca tgcgctcgac
      361 cctgcgccgg acggtcgatt ccgggatgcc ggttacccgg ctgagttcgg caaaggacac
      421 ccgcgcattt tgctggagca ggcggatcag tctgcgatcg gtggtcgaaa ccagccggga
      481 catgatgatg tccttccttc gaagccaggc gatagatcac atcatatggc gcacaggcca
      541 aagcgcaaca ggctcaaagg cccagcgctt ccgtgccgcg tcaacctacc acgacgacat
      601 ttgccgcggt agggcccgct ggcgggatgg gaatggctgc cggatggggg agtccgccag
      661 gtgactgcgt aactttgccc agattcgtcg acatacaggc actgaaaggg ggtttgtatg
      721 ctacagttcg ggtggtcagc ccgccggcct ggaggcatgt gcacatctgt cgcgatgctg
      781 aatggcgtaa cctgaagctg agtggtggcg aatgctttct ggtggcggca cggcgaacaa
      841 aggagttggg ggaggttcat gaccgttttg cgtaaggcca tggctggtgc gtttgtcctg
      901 gggttgctgg ttgcgcaact cggactgtca ggcacagcgc aggcggattc atccggcgtc
      961 gattcctata tcgagctgag tgcgaccaaa cccgcgtcgg gctgcaccat cgatgggtcg
     1021 ttcgaggttc gcagcgacgg atatccggtg gcgggcgttg aggtctttat cggcctctat
     1081 gtcggcaccg atctgatcag ctcggattcg gcgctgacgg acgataacgg cgttacctac
     1141 ctctcgatcg atcccagcgc cgcggatggc gcggacgcct ggatcgatgt caccctcaac
     1201 gacacctatt ggtggggcac cgcgtcctat ccgggcgctg gcggcacctg ctatgacggt
     1261 gctctcaacg cgaacaagtc cggccagatc gccggcgcac caagctcgac ggatgatgcg
     1321 tcgagcgcgc cgagcagcaa cgctcatgtc tccagcgatg cggtgatcat ccctgaggtc
     1381 ggtttctacg tgcagcagcg caacctgagc tgcgagtatg cggcggtcta cattgctacg
     1441 gctgcctggg gcaacggcat ctccgaatac gccaccgagg ccactgtcgg ctggtccgac
     1501 aacccgcacg aggggtatcg cggcaacatc aacggctggt ggggcaacac ctacgactat
     1561 ggcgtctacg ccgagccgct gtcctgggcc ctcgcgcagt acggcttcgt cggcgaggtt
     1621 gcctacacgc acggtgaccc gagctggttg atgaacgaga tcgcggccgg caacccgacg
     1681 atcgtctggc tcggcatgtg gggcgaccaa agcgtctacg aggccggcta tcgcgtcgtt
     1741 gccggcatgc acgtcatggt tgcctacggc tacgacgacg gcggtgtcta cttgtccgac
     1801 ccagccaccg gcggatcccg gtattacgac tggggcacgt tcgagtggat gtggggcgtc
     1861 atggacggca tgtccctctc ggtctatccc gcctgatcaa cagcaccaca tcgcaatgag
     1921 aaacggccgg tgagcaatca ccggccgttt tcgtctctgc accgcgcggg ccattcggtt
     1981 tatcatggca aaagttttgg tccgcaatgg ctgggacctc cgaactgttc cgatatgaca
     2041 caggtggcca atccgacgcc gtcggcgtcg gaccattggg cgattcttgc gctccgcgcg
     2101 ggtcgataga gtggcggcag tccctgtgat ccaaggctgc cgccctattg caatgcacgt
     2161 cgggaatgat ctgtgacgaa acaaaacatg gctggattga gccgccgcgc gctgatcggc
     2221 ggcgctttgg gaatggccgc gtcgatgacg agccgcatca tcgtcggggc cgaaacggct
     2281 ggcgtaccgg cgactgtcgg cggatcgacc atcaagctcc gcgtcagggc caggcgtgac
     2341 agcgacgtga ccgggagtat cccgtctaac gcgccggtca atgtgatcga gggaccgacc
     2401 ccggacggct ggtaccgggt cgagggcgaa acgaaacacg gcagccagcg cggctggaca
     2461 cacggcgacg ggctggtctt tgcgcagtcc gcggttttcc tctgggatgc gggcgtcttt
     2521 tcgggaccga ccgacgccac cggctggctg gccacgattc gacacggcgt cgtcgtgacg
     2581 gtggcaggac caggcagcaa cgggttcacg tttattcggt ttggcggcct gcagggctac
     2641 gtggcggcct ctgcgctgca actcacggat cagccggcaa cggacccact cggcgagtgg
     2701 tgggccgacg tgaatcgctc gacgctcacg gtcaacctca tgatcggttc gaccatcgtc
     2761 gactccttcc cggcggcgat gagcaccgaa acgggtgctg gcttctattc gacggcgccg
     2821 ggtacctact ggatctacga gaaggtcgcg gggctccaat acacgccgta cgccaaggcc
     2881 tacttcatgt actggtgcgg attcgatccg aatcggtaca acggcttcca ctcctggacg
     2941 atggacggca acgggtatgt ccttaacggc ggatggggca atacggccgg ctgcgtggcc
     3001 accgaaccaa agaatgcggc agtcatctac aacttcctca gcctcaacag ccggatcgaa
     3061 atccactggt gacgaggcag ttctgcactg ctctatagtc aaatagtccc gtgccgtctt
     3121 gacacctccc ccgaggtggt ggctagactt ccgtacccgc agggcattct cccgtggagg
     3181 atgaagcggt acggtggtga ttggactcct ggcgagtccg gtttcccgca ccgcttggca
     3241 gaggctggct acctccgtcc cctctgcgac acgaattgaa tgagggaaag gtggcatgct
     3301 gcacacgggt gttggcgccc gcgggctgtc gtggcattcg ctcagagtga atggtgcgtt
     3361 tggctggcga gtggttctgg tttcggttgt tctcttttcg ttgttctccg caacaatcca
     3421 accgtcgcgg gcggcgatcg acagctatgt cgataccgac gtgctctatc tgcgcacgga
     3481 cccgggcacg gacgccggtg tcatcgcgga gatgtatgac ggcgattacg tcgctgttat
     3541 cgatgggccg accgaagacg gctggtactt cctcgactat gagggcagcc agggttgggc
     3601 gtacggcgca tatctgaacg ttggcggctc gcctggctgg gagtcggccg cagcggatgc
     3661 gtcggtaggc ggcagcggcc cgaccgtttg ggtcgcgacc gatgcactca atatccgggc
     3721 cgagccaacc cggaagtcgt gggtgcttgg aacggtcgga cagggtgagt cgctttcgat
     3781 cgccggtgag aaggacaacg gcttctaccc ggtctggtac ggcgacaaca ttggctgggt
     3841 cgccggcaag tacctgagct gggagccggt gtccggcggt gaggagcggt ggatcgacgt
     3901 cgaccgcagc agctcgacca ttcgccttat ggtcggcatc gaatccgtcg gctattggtg
     3961 ggccgcgatg ggtttcgacg actccgataa cggcttctat tcgacggcag tcggcacgta
     4021 ctacgtctac gagaagaacg ccgcgctgac ctggaccgat tgggccaggg gctacatcga
     4081 atactgggtc gcgttcgacc cggaccggtt caacggcttc cactcctgga cgatggacaa
     4141 gaacggcaat gtcatcgagg gcggcgacgg cgcgaccggc ggctgtgtgg ctatggagcc
     4201 gggactggcg gcggcgctct atgaatttgc tacctatggg atgcgcgtcg agatccactg
     4261 gtagccaccc tccacattga tcgaacttcg aaggccccgg cgaaagtcgg ggccttttgc
     4321 cgcgtctcga cgcccgattg tcaccctgca tgggataatg ccggggcgta ttcgagcacg
     4381 atgaagcagg tcgatttgat gcaacgcagg tctctgttcg ttccccgccg tgccgtgatc
     4441 ttcctgatcc tgatcgcagc catgctcgtc gcgggcctgg gtgtcggatc ccctcagccg
     4501 gcggccgcga agtccgtcac ctggcgcaat tacgatgtca cgctgacgct gaacggtgac
     4561 agcaccttcc atgtggccga gcgccagatc atctcgttcg aagggggccc gttccatttc
     4621 gcttacgccg gtctttccct tgccgaggta gaggacttga cgggcatcaa ggtcagcgaa
     4681 atacgcaatg gatcgacgat tgcgtaccaa cagaacctgt cgggcgatcc ggaaacctac
     4741 tcccttgaac gcagtagcag cgccgtaact gttacgtgga acttccccac gacctcgaat
     4801 caggaccgca cgttcctcgt cgagtacgac gtgatcggag gcctccgggt ctacgacacc
     4861 gatgccgggc agcgccagca gatctggtgg aacgccgtca actccgacgt tacggacatc
     4921 gcgccggtgg attccgcttc attcaacgtt cagctccccc agcctgtggc taacctctcc
     4981 gatgtcgtga tcgatggatc cgatggttcc cgcgatccgg ccgcacacac tacggatgga
     5041 cgggtgtaca cctggacggc cacaaacctg ggggagggtg acacgctctg gggccgattg
     5101 gagttcccgg cgatggtcaa tgcgacggca cccgcctggc agaaatcggt cgacgagcag
     5161 cggcttcggg aacaggagcg gtcgagccgg gacgcgctcc tccggttgtt gctggccgga
     5221 gccgcgttgc tgacgctgac cgcgggtggt gtcggcctgt tcggtttctg gtacatgcgg
     5281 ggtcgggatc cgggcgtcgg ggcggtcgcg tcgtacctgt ccgagccgcc ggatgatttg
     5341 ccgccgggtg cggctggcgc gctgatcgat gaggaggtca acgagcggga tatcgtggcg
     5401 acgctcgtcg atctcggtcg tcgcggtgtg ctgaatatca agcaggaaga ggttggctcg
     5461 gtcttcaagc gcaacgagta caccatttct ctcaagcaag atgcgaagga tttgcggccg
     5521 ttcgagcaga ccttcgtcag ctcgatcatg agtggtactg aggcgggcaa gtctgtgacg
     5581 ctcggcgatg cgaaggagcg gttctcccgc aaggtcggca cgatcaggag tcagatgtac
     5641 gacgagctgg tcaagcgagg atatttcccc gtgtcgcccc agacgacgcg gacgcgctac
     5701 cagagctttg cgagtgcggc gatcgtcgga ttgatcatca tcgccgcgat ctttggcggc
     5761 gcgatcctcg acatcagcgg ctggttcatc gtcccggcaa tcgcgatcct ggtgattctg
     5821 ttctcgctct atcagaccgc gaagtacatg ccgcgaaaga ccgttgccgg tgcggaatcg
     5881 gcggcgaagt ggcgcgcatt caagagttac ctggctgatc tcgatcgaca aaagattggc
     5941 gacaatgccg gcgagatctt cgagaagtac ctgccctatg cggtagcgtt tggacttgag
     6001 cgaacctggg tgaccaggtt tgcggaggcg ggcgcgcaag caccagattg gtacggcgga
     6061 ttcgggggat acgggggcgg ctggttcgac ccacaggttg gtcgacgatc gggccggcga
     6121 acggtctggg tcggtggacc aggatggggc ggcatgccga cctccactgg cgcaccgcga
     6181 acaggcgacg gtgggggagt cgatatgccg ggtctgccgg atatccagtc cgcgagcgac
     6241 aagggcgcga agtcgcttca gtcgatgagc ggcggactct tcgatctctt cgacctggcc
     6301 ggatccgcct tcaagggatt cagcggctcg ggtaagagcg gcgggttcag cgggggcggc
     6361 cgcagcttcg gtggtggcgg cggcttcggc ggcggtggcg gcggtggagg cggcggccgc
     6421 gggttcggat gacccggcgc cgttttgtgc tcggctgccc ggtggacgtg ctcaatccgg
     6481 cagccttcaa ggcgtcgctt ccggcacttg ccagcggtga ttgcctgacc gatatcgtca
     6541 ccctgaaccc cgagcagatc atggccgccc ggcgcgaccc cgaggttgcg aggctgatcc
     6601 gaaaggcgca gctttgtacg gtcgacggta tcggtctgga gctcgcgttg cgggcacagg
     6661 gcgtgaccga cgtccagcgc atcaccgggg tcgatctcgt gacgtccgtc gtcgagcagc
     6721 ggataccgac gtttctgctc ggcggcgcgc ccggagcggc agaggaggct gggcggcgtt
     6781 tgaccgcccg gtttccgctg gcgcagatcg ccgggagctg gtccgggggg acgccgcgag
     6841 cgaaggacga tccacaatcg attgcccgga tcgcgctgtc gggagcgaag gcagtggcag
     6901 tcgcgtacgg ggctccggcc cagacggcgt ggatcgaacg gaatcgcgct gccctggagg
     6961 atgcgggcat tcggatcgca atcggtgtcg gcggatcgct cgatttctac gccgggtacg
     7021 tccgccgcgc gcccgagtgg gcacagcggt ttgggctcga atggctgttc cgactcatcg
     7081 ctgagccatg gcggctgcgg cggcaactcg ttctaccgaa attcgccgtt ctcgccttga
     7141 tcgaggccgg acgcggccga attggacacg cttgacgtat gctcttcggt atgcaatttg
     7201 ccaacctcgt gagcatgctc tatcgatgac tgttaccgaa acccccgtat tcaccactga
     7261 ggacgtgccc tgcgcgcgcc atcccaaagt acagacgcgg ttgcgctgct cccggtgcgg
     7321 gacgccgatc tgcccccagt gcgcggtccg cactcccgtc gggttgcgtt gccccgactg
     7381 cgccggcgtg cgcggcctgc cgacctactc cacccccggg gcgtcgatgc tgaaggcggc
     7441 tggcgcgggc gtggcggtcg ggcttgtctt cgcgctgctc ttcgcctgga tcccgcagtg
     7501 gaacttctac ctgtcgctgg ccttggggtt cggcgtcgcg gaggcgatgg cgcgggcggc
     7561 gaagaacaag cgtggcgccg acctgcaggt cctcggcatt gtcatcgcgc ttggcgcgat
     7621 gggactcggg cgggcgatcc tggcctggcg cgaggatttg acgtgggcgc aagtcaacgc
     7681 cttctctcca tacgtcgaga atctgttgcg catgtcgctc acgccggatg ggttgtttgc
     7741 cgcgctgacg gtgttgatcg tctggtaccg gttccgctag ccacccgatg catgcgccaa
     7801 gtcgctccgg ggttgccagg gccgcgctgc tgatcacggc cggcggactt gccagccgca
     7861 tcctcggcct cgtccgcgag cagctcgccg ccggctattt tggtgccggc gataaggttg
     7921 cggccttcca gatcgccgac aacgtccaga ccttgctctt cgacctcgtc atcagcggga
     7981 tgctccaggc ggccctggtg cccgtgctgg tcgagttcgc cggaccgggc aatcgtcaga
     8041 tcctgcggcg catctccggc acgatcgcga cggtcgccgt cgtactcgtc ggtctcgcca
     8101 cggtgatcgg gtggctgttc acgccgcaac tcgtgagcgt gatgacgtcg ctcggggccg
     8161 gcgatcaaag ccgctcgcct gagacggtgg cgatgaccga gcacatggtc aggatcgtcc
     8221 tgccgggcgt gctcttcctg gcgctcggga ctgtcttctc ggccgtgctc tattcgctcg
     8281 ggcgggcatc cggaccagcg acggctctgg ccgcgagaaa cctggtcgtg gttgcctcga
     8341 tcctgctgct agcgaaccgg ctcggggtcg agagcatggc ctatggcgtg ctgattggcg
     8401 gcgcgctggt ggcggtgatc cagttgccgt ggctgatgca gcacgatgct gtcccaatcc
     8461 cgaacctgca tctcagggat ccagcggtcc ggcatatcgg gcggctctac attccggtct
     8521 ttctcggcct gatggtcagc accgtccagg tggtggtcga tcgcaacctt gcctggcgcg
     8581 cggaatcgga cgcgctcggg gcgatgcgct acgcgacgac attggtgcag tcggtactcg
     8641 ggcttgtcgc ggccgcgatc tcgctggccg ccttgccggt attggcgacg catttctcgc
     8701 agcgcgacga ggagcggttc gaccagacgc tcgttcaggc ggtgcgcatg gtgacggtcc
     8761 tgatcgtacc ggcggtattc gggttggccg ccctctcgcg accggtcgtc ggtttgctgt
     8821 tccagcatgg cgagaccggc cccgccgagg cccattcgat cgctgtcgtc ctgctcgctt
     8881 atctgccggg tacgctcttc gccgcgtatg accagatcct gatttacatg ttctactcgc
     8941 gccgctcgac ctggatcccg gtaatggtcg gcgtgctctc ggtcggtgcc tatttcgtgg
     9001 ccgccgcgct cctcgcgaac cggtatggcg ctgtcggtct ggcaaccgcg aacagcgcgc
     9061 agttcgtgag ccatactctc gtgcttggct tcatcgcgcg gaaacgggtg ttgagcgtct
     9121 ggggccaatt ctccggcctg ctggcactga caatcgccgg ttcagtggcc tgcgcagcgg
     9181 tcgcatatct cagctaccgt ggtattgccg ggacgacagg cggaatcgtc cgcgagatcg
     9241 gggcggtgct tattccggcc gcgttgggcg gcgcggttta cctggcggct ctgctggccg
     9301 cgcacgtgcc ggagatccac gtgatcaaga accgcgtcgg agcccgtctt ccggctcgat
     9361 aggatccaac cgggccagcg cgcccgaggc tccgacccgt cgtccgatgt acggctgccc
     9421 gaggtggtat gcctcgttcc agacgtcgcg cacggccaca tcctgggccc gggcgatggc
     9481 ggcgcagtcg tcgtattccg gggcgaggtc gatcacgcgg ccatgccaga tcttgagctt
     9541 gacccggacc gatccccagc gagtcagtac cgcctccatc tgccgatcgg ccgcgatgcg
     9601 gttcacctgc gtttggcgga cgccgagcgt ggtcgactgc tggatcagca acgcggtcag
     9661 ctcatcggcg gcgctcggtc gggcgagcgc gctgatcatg accgccgggc gccccttctt
     9721 catgccgatc gctgtcgtcc aggcatcgag ggcaccggcg gcgaagagtc gctcgatcag
     9781 aatctcgacg aactgcgggt tcatatcgtc gatgttggtc tcgatcacga ccagcgattc
     9841 gccgccggac ggccccgctt cggtctcgcc gatcacgatc cggcagatgt tggcccacgg
     9901 cagttccttc gtgccgaaac ctgagccgac tgcctggatc gtcatagccg gccggtcgaa
     9961 cgaggcgagc gtgcccagga tcgccgcggc ggtcggggtc agtagctcgc cggcctcagt
    10021 ctcgcccggc atcggcgtcg caattagcgc ggtagacatg gccagcagcc gggaggtggc
    10081 cggcgccgga accgggagca gcccgtgcgc ggtcttgacc gtgccgccgc cgacccggag
    10141 gggaccgcat gaaatgcgct cgacgccgag caagcggaag ccggctacca caccgacgac
    10201 atcgacgatc gtgtcgagtg cgccgacctc gtggaagtgg accttgtcga ccggcacggc
    10261 gtgtaccgat gcctcggcct cggcgaggcg cgcaaagatc cgcactgcgt tgtcacgcac
    10321 gactgcatcg agattcgcgc gctggatcag ggctcggatt tcgctccagg tacgcgagtg
    10381 gtgcccttgt tccggtggga cgctcgcgtg ggttccggtg atgccgttgc tggtga
//
LOCUS       DATAOZ010000337         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1981514, whole genome shotgun sequence.
ACCESSION   DATAOZ010000337 DATAOZ010000000
VERSION     DATAOZ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981514"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VHR64_08670"
     CDS             complement(<1..392)
                     /locus_tag="VHR64_08670"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3302939.1"
                     /translation="MIRTGDEYRESIRDGREVWMNGEKIEDVTTHPAFKPAVDARADI
                     YDMQHRPECQETMTYVDEGTGERCAIALKLPMTRQDWRDKRRAVDAVLDEVGGVVTRV
                     GDETIGEMWSLYDGQDVLNEVNPQFSEN"
     gene            complement(473..1063)
                     /locus_tag="VHR64_08675"
     CDS             complement(473..1063)
                     /locus_tag="VHR64_08675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX3302940.1"
                     /translation="MSEDRPVELRAAFIEAMSRAATGVTIVTTDAPDGRFGQTVSAMC
                     SVSADPPSLLVCIKTTSVIVNAIRQRRAFGVNLLRADQRRVADTFAGRRSWGEPYDFE
                     IATWDSLETGVPLLVGAVGRFDCRLDTVLEVGSHSVLFGDVAAADSAAGTPLLYARRS
                     YGEHMEIPRQVDEGMLDLDVRLTWEDLDKGPRREGE"
     gene            complement(1101..1970)
                     /locus_tag="VHR64_08680"
     CDS             complement(1101..1970)
                     /locus_tag="VHR64_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX3302941.1"
                     /translation="MYGKTVKPGSRVSIKQLKTDLPDAIEKDVGEAVFGEYSKEIGEL
                     QELLFAAGTHALLVIFQGMDTSGKDGATKGVFREVNPSGCRVVSFGVPSEEELAHDFL
                     WRIHQKTPARGQIVVFNRSQYEDVLVVRVHDLAPKEVWSKRYDQINAFEKLLTDTNTI
                     VLKFFLHISREEQEERLLEREQNPVKSWKLSVGDWKERTHWTDYQEAYQDALNKCSTE
                     HAPWHVIPADQKWFRNLAISEIVVTELRKYRDDWEKHLAELSKTQAAALAEARKEGSI
                     PKPTQRAVAAVDE"
     gene            complement(2154..3212)
                     /locus_tag="VHR64_08685"
     CDS             complement(2154..3212)
                     /locus_tag="VHR64_08685"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX3302942.1"
                     /translation="MTTSALDYADFDGLWTAFHRGADAPLGQLFMGVRTTGIYCRPGC
                     PARQPKRTNVRFFATASAAERSGFRACKRCHPNELQPNPKLAMVESACRYIDEHYDDD
                     VRLEALADALGVSTQTLQRTFREVIGISPRVYARERRLDQFRDELRGGSDVSQAIYGA
                     GFSSPSRIYERAGDDLGMTPAQYRRGAPEEAIRVALGQSPFGIVGVGRTEAGICSIRL
                     GDSPDDVLSAITSELPHAEIALVESDPDLDRIVAHVVAGTSITNLSLDLRGTAFQRKV
                     WTELRRIPRGEQRTYQQIAQQVGSPKSVRAVANACGANPTALAVPCHRVVRTDGTLGG
                     YRWGVERKQAILELEQAG"
BASE COUNT          558 a         1042 c         1088 g          635 t
ORIGIN      
        1 atgttctccg agaactgcgg gttgacttca ttgaggacat cctggccgtc gtacagcgac
       61 cacatttcgc cgatcgtctc gtcgccgacg cgtgtgacga cgccgccgac ctcgtcgagc
      121 acggcatcga ccgcccggcg cttgtcccgc cagtcctgcc gggtcatggg caacttgagg
      181 gcgatggcgc agcgctctcc ggtgccttcg tcgacgtagg tcatcgtctc ctggcactca
      241 gggcggtgct gcatatcgta gatgtcggcg cgggcatcaa cggcgggctt gaaggcagga
      301 tgcgtggtga catcctcgat cttctcgccg ttcatccaga cctcgcggcc gtccctgatc
      361 gattcgcggt actcgtcgcc ggttcgaatc acgcatgtac tccttcagtg gtcagtgttc
      421 agtggtcagt gacgagtttc ctgtttccag ccaaaacttc gcgccttcgc gactactcgc
      481 cttcgcgcct cggccccttg tccaggtctt cccaggtcaa tcgcacgtcc agatcgagca
      541 tgccctcgtc gacctggcgc ggtatctcca tatgttcgcc gtagctgcgg cgagcataaa
      601 ggagcggcgt gccggctgcc gagtcggccg ccgccacatc gccgaacaag acggaatgtg
      661 agccgacctc gagcacggta tcgagacggc agtcgaaccg gccgaccgcc ccgaccagga
      721 gcggaacacc ggtctcgagc gagtcccagg tcgcgatctc gaagtcgtac ggctcgcccc
      781 agctccggcg accggcaaag gtatccgcga cgcgacgctg atcggcccgg agcaggttga
      841 cgccgaacgc ccggcgctga cgaatcgcgt tgacgatcac gctggttgtt tttatgcaga
      901 cgagtagcga cgggggatcg gccgagaccg agcacatcgc gctcacggtc tgaccaaacc
      961 gcccgtccgg ggcgtcggtg gtgacgatgg tgacgccggt agcggcccgg ctcatcgctt
     1021 caatgaacgc ggcccgcagc tccaccggtc tgtcctcgct caagtgatga atccgggcgg
     1081 attgtcgcat ggagcagaag tcactcgtcg accgctgcga cggctctctg cgtcggcttc
     1141 gggatcgaac cctctttgcg ggcttcggcg agagcggcag cctgggtctt gctgagctca
     1201 gcgaggtgtt tctcccagtc atcccggtat ttgcgaagct cggttacgac aatttcggaa
     1261 atcgccagat tacggaacca tttctggtcg gcagggatca cgtgccacgg agcgtgttcg
     1321 gtcgagcact tgttgagtgc atcctggtag gcctcctggt agtcggtcca gtgcgtgcgc
     1381 tctttccagt cgccgaccga aagcttccag gatttgaccg ggttctgctc gcgctcgagg
     1441 aggcgttcct cctgctcctc cctgctgatg tggaggaaga acttgagcac gatcgtattg
     1501 gtgtcggtga gcagcttctc gaaggcgttg atctggtcgt agcgcttcga ccacacctcc
     1561 tttggggcga ggtcgtgcac gcggacgacg agcacatcct catactggga gcggttgaag
     1621 acgacgatct ggccgcgagc cggggtcttc tggtggatac gccagagaaa gtcgtgggca
     1681 agctcttcct ccgacgggac gccaaatgag acgacccggc agccggaggg gttgacctcg
     1741 cggaagacac ccttggtggc gccgtccttg ccgctggtgt ccatgccctg aaagatgacg
     1801 agcagggcgt gagtgccggc cgcaaagagc agctcctgaa gctcgccaat ctctttcgag
     1861 tactctccaa agacagcttc gccgacatcc ttctcgatcg cgtcgggcag gtcggtcttg
     1921 agctgtttga tcgagaccct gctgccgggc tttactgtct tgccgtacac gcgctctccc
     1981 tgaccttcac ctgcctatcc agattgatga caaatcgtag cgcgggactg ccgtgccgcg
     2041 ctgtggccgt aggtcacggt agtttcgcca gaagaccgat cccgcatagg taccggtctt
     2101 ccggatactc acccgcggca cggcagtccc gcgctacgtg tgaagtccag ccgctagccg
     2161 gcctgttcga gctcgaggat agcctgcttg cgctcgacgc cccagcggta gccgccgagg
     2221 gtgccatcgg tgcggaccac acggtggcag gggacggcaa gggcggtcgg gttcgcgccg
     2281 caggcgttgg ccacggcccg gaccgacttc ggactgccga cctgctgggc gatctgctgg
     2341 taggtgcgct gctcaccacg cgggatccgg cgcaactcgg tccagacctt ccgctggaac
     2401 gccgtgccgc gcaagtcgag cgacagattg gtgatcgatg tgccggcaac gacgtgcgcg
     2461 acgatccggt caaggtcagg atcggactcg acgagcgcga tctcggcatg cgggagttcg
     2521 ctcgtgatcg ccgagagcac gtcgtcaggc gagtctccga gacgaatcga gcaaatccca
     2581 gcctcggtcc gtccaactcc gacgatgcca aatgggctct ggccgagggc gacacggatc
     2641 gcctcctcag gcgcgccgcg gcggtactgg gcgggcgtca tgccgaggtc atcgccagca
     2701 cgctcgtaga tgcggctcgg cgagctgaag ccagcgccgt agatcgcctg gctgacatcg
     2761 gagccgccgc gcagctcgtc gcgaaactgg tcgagacggc gctcacgggc gtagacgcgc
     2821 ggtgagattc cgatcacttc gcggaaggtg cgctgcaacg tctgcgtgct gacgccgagc
     2881 gcatcggcca gagcctcgag ccgcacgtca tcgtcgtagt gctcatcgat gtagcggcag
     2941 gcagactcga ccattgccag cttcgggttg ggctggagct cgttcgggtg gcagcgcttg
     3001 caggcgcgga atccggatcg ctcggccgcc gaggccgtcg cgaagaagcg gacgttggtg
     3061 cgcttcggct ggcgggcggg gcagcccggt cggcaataga tgccggtcgt gcggacaccc
     3121 atgaagagct ggccgagcgg cgcatcggcg ccgcggtgga aggcggtcca gaggccgtcg
     3181 aaatcggcgt agtcgagcgc gctggtagtc atggtcacgg cattcggctc ctttcgaatc
     3241 gtctttgcat cgattctgga gggcataccg gacgaatact atccgctttg cgtcagcaca
     3301 tcaggaatcg cgttgattgc gct
//
LOCUS       DATAOZ010000338         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6976061, whole genome shotgun sequence.
ACCESSION   DATAOZ010000338 DATAOZ010000000
VERSION     DATAOZ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6976061"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /gene="iadA"
                     /locus_tag="VHR64_08690"
     CDS             complement(<1..1165)
                     /gene="iadA"
                     /locus_tag="VHR64_08690"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEX3302943.1"
                     /translation="MFTVITGAEVYAPQPLGVVDVLIVGERIAAVGKDLARNLGAIGA
                     GAEVIDLTGRILTPGLIDGHYHPLGGGDYEGPLGRVTDIELGDIVRAGITTAVGVLGS
                     DYDARNLNDLFMKSRELQMGGITAFFYTGSFFLPPVTLTGAVRRDIMVVESCIGVKFA
                     ISEEMAFNDEMDMAKVAVEGVHGGSLAGKPGIVHMHVGERASSLDPLFAMIERTGLPA
                     HRFYPTHINRSDPDHMKPAAKFVEMGGTIDLTAIMCPRGGSGTGVRIAEAFANLLNLG
                     VPVERITFTSDGNVSMPIRNERREQIGLFNAGVDFLLDEWQDTVRESGLPFEQMLLPV
                     TINPARVLKMTGRKGCIAVGADADLVAWDDALCPQTVIARGKTMLHHGELLAKG"
     gene            1332..1586
                     /locus_tag="VHR64_08695"
     CDS             1332..1586
                     /locus_tag="VHR64_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302944.1"
                     /translation="MKSKDVMLGSLRLNLNATRAGVAISYYDQDYTNHGGLMRVRIGY
                     LTADELRSGEADVAIDITPILPVAAEMFTAAGGKLNEDAA"
     gene            complement(1618..1842)
                     /locus_tag="VHR64_08700"
     CDS             complement(1618..1842)
                     /locus_tag="VHR64_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302945.1"
                     /translation="MFYPTFMVPMIISALIWRRWTIPFVAIGSALAFFPGREAIGIPL
                     YLTTLGAMTGLPLRWGIAELIDELTPHRHA"
     gene            2064..2714
                     /locus_tag="VHR64_08705"
     CDS             2064..2714
                     /locus_tag="VHR64_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302946.1"
                     /translation="MPTPTWDGSSVSPDHVRRAAAICHDTLAPALGADWSIPAGPLTW
                     TCRETLDHITDALAYYCGQIAMRAPGRRPSIRNGDPAASISDLLDSVVTSAGILAAVA
                     EATPGNERAYHRMGMADAEGFLAMACEEILVHTWDIAQGFGLSVAVPDDLAGAVLHRI
                     FPWAPTGCPAWEAQLWCSDRIELPGKGKIGQWGWHGAPLSEWDGQMRPAITFPVES"
BASE COUNT          537 a          949 c          960 g          469 t
ORIGIN      
        1 ggcctttcgc gagcagctcg ccgtggtgga gcatcgtttt gccgcgcgcg atgacggttt
       61 gcgggcagag ggcgtcgtcc caggcgacga ggtcggcgtc ggcgccgacg gcaatgcagc
      121 ccttgcggcc ggtcatcttc aggacgcggg ccgggttgat cgtgaccggc aagagcatct
      181 gctcgaaggg caggccgctc tcgcggaccg tgtcctgcca ctcgtcgagc aggaaatcga
      241 ccccggcatt gaagaggccg atctgctccc ggcgctcgtt gcggatcggc atcgagacgt
      301 tgccgtctga cgtgaaggta atccgctcga ccggcacgcc gaggttgagc aggttggcga
      361 acgcctcggc gatccgcacc ccggtgccag agccgccgcg cgggcacatg atcgccgtca
      421 ggtcgatcgt gccgcccatc tcgacgaact ttgccgccgg cttcatgtgg tcgggatcgg
      481 agcggttgat gtgggttggg tagaagcggt gcgccggcag gccggtccgc tcgatcatcg
      541 cgaagagcgg atcgaggctg gaggcgcgct cgccaacgtg catgtggacg atacccggct
      601 tgccggcaag gctgccaccg tgcacgccct cgaccgcgac ctttgccatg tccatctcgt
      661 cgttgaaggc catctcctcg gagatcgcga acttcacgcc gatgcaggac tcgacgacca
      721 tgatgtcgcg ccggaccgcc ccggtcagcg taaccggcgg caggaagaat gagccggtgt
      781 agaagaaggc cgtgatcccg cccatctgca gctcgcgact cttcatgaag aggtcgttga
      841 gattgcgggc atcgtaatcg gacccgagca cgccgacggc ggtcgtgatc ccggcccgga
      901 cgatatcgcc gagctcgata tcggtgacgc ggccgagcgg accctcgtaa tcgccgccgc
      961 cgagcgggtg gtaatggccg tcgatcaggc ccggcgtgag gatccggccg gtgagatcga
     1021 tcacctcggc cccggctccg atcgcaccga ggttgcgggc gagatccttc ccgacggcgg
     1081 cgatccgctc cccgacgatc aggacatcga cgacgccgag cggctgcggg gcatagacct
     1141 cggccccggt aatgacagtg aacatggaga acccccgagc gatgcgtgaa tgacggcggg
     1201 attgtacggg agtcgagcag tcgagcagcc ggaaaaccaa agaccagaga caaaacaacc
     1261 cgatgcacgt acagaaccga tgttctatac ttgaccggta gacacgcgac ctgcagagag
     1321 gacgacgaga tatgaaaagc aaggatgtga tgctggggtc gctgcggttg aatctcaacg
     1381 cgacccgggc cggggtggcg atcagctatt acgaccagga ttacacgaac cacggcggcc
     1441 tgatgcgggt ccggatcggc tacctgacgg ccgatgagct gcgctccggc gaggcggacg
     1501 tcgcgatcga catcacgccg atcctgccgg tcgcggccga gatgttcacc gccgccggcg
     1561 gcaaactcaa cgaagacgcc gcctgagctg agtttagggt tgagggaacg gaaaacccta
     1621 cgcgtgacgg tgcggcgtga gttcgtcgat cagttcggcg atgccccagc gcaggggtag
     1681 gccggtcatc gcgccgaggg tggtcagata gagggggata ccgattgctt cacgaccggg
     1741 gaagaacgca agggccgaac cgatcgcgac aaaaggaatc gtccatcgcc gccaaatcag
     1801 cgcggagatg atcatcggca ccatgaaggt cggatagaac atcacccctt ccgagcctcg
     1861 gattttattc taccggcacc cgaacaagag gccacccctc ccctgttcac gggagaagat
     1921 gggcaaccga ttcgcatacg tcgcaccggt cgtctttcgg cccgagtcgc attgccatgc
     1981 gacgctacgg ggcgccttcc ctttcccccc tttccctcaa ccctccaccc caccagtaca
     2041 atcccggcag gaggagccag cgcatgccga caccaacctg ggacgggtcg tccgtctcgc
     2101 cagatcatgt ccgccgggca gccgcgatct gtcacgacac cctcgcgccc gcactcgggg
     2161 ccgactggtc gatcccggcc ggtccgctca cctggacctg ccgcgagacg ctcgaccaca
     2221 tcaccgacgc gctcgcctac tactgcgggc aaatcgcgat gcgcgcgccg ggtcggcgac
     2281 cgtcgatccg caatggcgat cccgcggcat ccatttcgga tctgctcgac tcggtcgtga
     2341 ccagcgccgg cattttggcg gcggtggcgg aggccacacc agggaacgaa cgcgcctacc
     2401 atcggatggg gatggcggac gccgagggct tcctggcgat ggcctgcgag gagattctgg
     2461 tccacacctg ggatatcgca cagggcttcg ggctgtccgt cgcggtcccg gacgatctcg
     2521 ccggcgccgt tctccaccgc atcttcccgt gggcgccgac cggttgcccg gcgtgggagg
     2581 cgcagctctg gtgctcggac cggatcgagc tgcccggcaa gggcaagatc ggtcaatggg
     2641 gctggcacgg cgccccgctc tccgagtggg acggccagat gcgcccggcg atcacgttcc
     2701 cggtagagag ctaggcgacg ccaacctgta gggatttgcc gtggcaaatc tgcaatggac
     2761 cctgtgaccg gcgccaatca ggtatccagc aacagacagc gggcggttcg gccgcacccg
     2821 gatttgccac ggcaaatccc tacagcgttc acgcaacccg ctactccccc gtatacagcg
     2881 ggcggcgctt ttccttgaag gcggtcatgc cttcc
//
LOCUS       DATAOZ010000339         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_664416, whole genome shotgun sequence.
ACCESSION   DATAOZ010000339 DATAOZ010000000
VERSION     DATAOZ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664416"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1408
                     /locus_tag="VHR64_08710"
     CDS             326..1408
                     /locus_tag="VHR64_08710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3302947.1"
                     /translation="MITQSPAEVVTDRLFGAMLQMWDVASVFLGARLGYYEALAELGE
                     ANPCQLAERVSANERYTREWLEQQAATGLIEVATATDDDNARTYRLIPGTEPVFTDGT
                     GEQPILHFVRTTLASILATPDVITAFRTGKGLPFGHYGEDMMIGQGLGNRWGFMTDLA
                     TTWIPAMPDIDERLRTKPDARIADIGFGAGWSSIGFAQAYPNVRVDGLDLDEASARAA
                     QKNADEAGVGDRVTFMAKDAADPALSGRYDLAFAFECVHDMANPVAVLRAMRNLVGPG
                     GTVLIVDEKVGDTFTAPADDVERFMYGYSIFHCLPGAMDGENPAGTGTVMRQPIFRRY
                     AADAGYQSVDILPIENDFFRFYRLTA"
     gene            1650..2009
                     /locus_tag="VHR64_08715"
     CDS             1650..2009
                     /locus_tag="VHR64_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302948.1"
                     /translation="MTHRGALLASIALTIAVALVLGSQWDLITTRGSQADPTPTPAAP
                     AQDFIDPNSSGATDSTGEPGLAAQSGLTLDALNDTSTAPQTNSPAFDAQGSWNDDEGE
                     HDGDYVDEDHENDEHDD"
     gene            1990..2382
                     /locus_tag="VHR64_08720"
     CDS             1990..2382
                     /locus_tag="VHR64_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302949.1"
                     /translation="MTSTTTEYPSWPRRLPAHPNSLERKAVLVHGMSDPLGAKGFRQS
                     RDRIEREGKARRLAAVGAIATFVASLVLIVRDGSHSTPINDTSGIQQAPVGSQSQTTG
                     LGARAPGLEEQIQVQVPAQSHTRTRSSD"
     gene            2624..>2918
                     /locus_tag="VHR64_08725"
     CDS             2624..>2918
                     /locus_tag="VHR64_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX3302950.1"
                     /translation="MGSRIELIAVGVDNAVADRALATAREFAEEWEKLFSRFRPQSEL
                     SRLNESAGRPQAVSTEFLVVFDAARAAYERTGGLFNPSILPSLTALGYDRDFRE"
BASE COUNT          512 a         1009 c          919 g          478 t
ORIGIN      
        1 gtacccgatc cctgtctctt aatcccacac ccgaactgta ggggcagatg gcacctgccc
       61 gcgcctcgcc caaatccccg tggcatttac caaccctgtc accgatctgt gtttccgggg
      121 gtatgcgggc ggaaccacgc cgggcagatg ccatctgccc ctacggagct ccgctcctca
      181 actgctcgcc tgctcgcctc ccgactgctc gcctcattct cgtgacaaaa cgtcatcctc
      241 gttataggat cgatcgcgtt ctgcttgtca ttgcgatctg accggtccga gtcacgtcgt
      301 ccgcgggggc gaagcgaggg gttcgatgat cacgcagtca ccggctgagg tcgtcactga
      361 tcgtctgttc ggagcgatgt tacagatgtg ggacgtcgcg tcggtcttcc tcggcgcgcg
      421 cctgggctac tacgaggcgc tggccgaact gggcgaggcg aacccgtgcc aactggccga
      481 acgggtcagc gccaacgagc gctacacccg cgaatggctc gagcagcagg ccgcgaccgg
      541 tctgatcgag gtcgcgaccg ccacggacga cgacaacgcc cgcacctacc gcctgatccc
      601 gggaaccgag ccggtcttca ccgacggcac gggagagcag ccgatcctcc atttcgtccg
      661 tacgaccctg gccagcatcc tcgccacgcc ggacgtcatc acggcattcc ggaccggcaa
      721 gggcctcccc ttcggccact atggtgagga catgatgatc gggcagggac tgggcaaccg
      781 ctggggattc atgaccgatc tcgccacgac ctggatcccg gccatgcccg atatcgacga
      841 gcggttgcgg acaaagcccg atgcccggat cgcggatatc ggcttcggcg caggttggtc
      901 atcgatcggc ttcgcgcagg cgtatccgaa cgtgcgggtc gatgggctgg atctcgacga
      961 ggcttctgcg cgggcggcgc agaagaatgc cgatgaagcc ggcgtgggcg accgggtcac
     1021 gttcatggcg aaagacgcgg ccgacccggc gctctccgga cggtacgacc ttgccttcgc
     1081 cttcgagtgc gtccacgaca tggcgaaccc ggtcgccgta ctgcgggcga tgcggaacct
     1141 ggttggcccg ggtgggaccg tcctgatcgt cgacgagaag gtgggcgaca ccttcacggc
     1201 gccggccgac gatgtcgagc ggttcatgta cggctacagc atcttccact gcctgccggg
     1261 cgcgatggac ggcgagaacc cggccggcac cggcacggtc atgcgccagc cgatcttccg
     1321 gcggtacgcg gcggacgccg gctaccaatc ggtcgacatc ctgccgatcg aaaacgattt
     1381 cttccgcttc taccggctga cggcgtaacc gaggcgagaa gttggtcgtt ggtcgttggt
     1441 cgttggtcgt tggtcgttgg tcgttggtcg ttggaatgag cgtactggct tccttcctgt
     1501 tccatctacc aacgtccaac gtccatccac ccgactgctc gcctccccat caaattcccc
     1561 tctaatcctg ccgccataac ctgacctcac gatgcggcca ggcttgaatc gctccagcgc
     1621 accgcatcag caggaaacgg ggacacccca tgacgcaccg gggcgcattg cttgcctcca
     1681 ttgccctcac catcgcggtc gcgctcgtgc tcggctcaca atgggacctg atcaccaccc
     1741 gcggatcgca agccgatccg acaccgactc cggccgcacc ggcccaagac ttcatcgatc
     1801 ccaacagctc cggggccacc gactccaccg gcgagccagg tcttgccgcg caatcgggcc
     1861 tcacgctcga tgcgctcaac gacacctcca cggcgccgca aaccaactcg cccgcattcg
     1921 atgcccaggg aagctggaat gacgacgagg gcgagcacga cggtgactac gtcgacgagg
     1981 atcacgagaa tgacgagcac gacgactgag tacccctcct ggccgcgacg actgcccgcg
     2041 cacccgaact cgctcgagcg caaggcagta ctcgtccacg gcatgtcgga tccgctcggc
     2101 gcgaagggct tccgccagtc gcgcgatcgg atcgagcgcg agggcaaggc acgccggctg
     2161 gcggccgtcg gcgcgatcgc gaccttcgtc gccagcctgg tcctgatcgt tcgcgacggc
     2221 tcgcacagca cgccgatcaa tgacacctcc gggattcagc aggcgccggt cgggagccag
     2281 agccaaacca ccggtctcgg cgcccgtgcc ccaggcctgg aggagcagat ccaggtccag
     2341 gtcccggccc aatcccacac ccgcacccga tccagtgact agcgtgaagc gcggccgcgg
     2401 tgaccggcgc caacacgtag cgcggggatg ttttctcccc gatggggaga gtcggagagc
     2461 ggatcacgcc ccgcccgggc cagacgcgcg gcaatgcgtt tggggaacca accccgtcgc
     2521 cggttcggtt ccatcaaccg tgcttgggga tgaacaagcg gggcgtggta accccgcgct
     2581 acgaaccgaa aaagggacaa ggcgccgccg cgaatttcgg gcgatggggt cccggatcga
     2641 gctgatcgcg gtcggtgttg acaacgcggt ggcggatcgg gcgctggcga ccgctcgaga
     2701 gttcgccgag gagtgggaga agctgttcag ccgcttccgg ccgcagagcg agctgtcccg
     2761 cctcaacgag tccgccggac ggccgcaggc ggtctcgacg gagttcctgg tcgtattcga
     2821 cgcggcccgc gcggcgtatg agcggaccgg cggactcttc aacccctcga tcctgccgtc
     2881 actgaccgcc ctcggctacg accgcgactt ccgagagc
//
LOCUS       DATAOZ010000340         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_664491, whole genome shotgun sequence.
ACCESSION   DATAOZ010000340 DATAOZ010000000
VERSION     DATAOZ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664491"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1072)
                     /locus_tag="VHR64_08730"
     CDS             complement(38..1072)
                     /locus_tag="VHR64_08730"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX3302951.1"
                     /translation="MVDVAIARIVECHGSPAAMGEMHGEATRDLIAETVDAWRTLISG
                     RAPAEFDPIARLLAETNFLPAIDRHAPDLLEEVRGIARGANQPFETMLVYNLMDEEWS
                     FRTGRLGPGCTAIAVEGVGIAQTMDIPSVHDGSQVALRLRPDHGSEQIVFTGAGMLGL
                     NGANRDGVGVVVNNLAQLPASSTGLPVMFMLRSILGQKSAAEAGAFVKSVPHAIGQHY
                     LIGDPDQIISLEAAANGVYEVPVAERYVHANHPLANPASRSEAADMERRSNTHARSNA
                     AKSLVREGSDQRDIECALEDRTAPISCAKRDGSMTFGGTSIELTSEPRVRIAFGPPHE
                     APWTDIPWNS"
     gene            1239..>1577
                     /locus_tag="VHR64_08735"
     CDS             1239..>1577
                     /locus_tag="VHR64_08735"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX3302952.1"
                     /translation="MAAKRDKVIISCAVTGAVHVPSQSDYLPITPEQIAENAIGAAEA
                     GAAILHLHARDPQTGRPTPNPDMFMQFLPEIKERTDAVVNITTGGGQGMTLEQRLAAA
                     MRAKPEMCSLN"
BASE COUNT          307 a          522 c          468 g          280 t
ORIGIN      
        1 gatcacaccg acctgtctac ccgccacgga ccgcccgcta cgagttccac gggatgtcag
       61 tccagggcgc ttcgtgtggt ggtccgaatg caatacggac ccggggctcg gaggtcaatt
      121 cgatactggt gccgccgaaa gtcatcgaac cgtcgcgttt ggcgcagctg atgggcgcgg
      181 tcctgtcctc gagcgcgcac tcgatatcac gctgatccga tccctcccgc acaagcgatt
      241 tcgccgcgtt gctccgggca tgcgtattcg aacggcgctc catatcggcc gcctcgctcc
      301 gcgacgccgg gttggcaagc ggatgattcg cgtgcacata ccgctcggca acaggcacct
      361 cgtacacccc gttagcggcc gcctccaggc tgatgatctg atctggatca ccaatcagat
      421 aatgctgacc gatcgcgtgc gggactgatt taacgaatgc gccagcctca gcggccgatt
      481 tctggccaag gattgaccgc agcatgaaca tgaccggcag ccccgttgag gacgcaggca
      541 attgggcaag gttgttgaca acaacaccga cgccatcgcg gtttgcgcca ttcaacccaa
      601 gcatgcccgc gccggtgaag acgatctgct cagatccgtg gtcgggtcgc aagcgcaagg
      661 caacctgcga gccgtcgtgc accgagggga tatccatggt ctgggcaata ccgacaccct
      721 ccacggcgat cgcggtgcat cccgggccga gtcgacccgt ccgaaaactc cactcctcat
      781 ccatcaagtt gtagaccagc atggtttcga agggctggtt ggcgccccgg gcgatgccgc
      841 gcacctcttc gagcaaatcc ggagcatggc gatcgatggc gggcagaaaa ttggtctccg
      901 ccaatagacg agcgatcgga tcgaattccg ccggagcgcg ccccgaaatc agcgttcgcc
      961 acgcatcgac ggtctcggcg atgagatcgc gcgtcgcctc gccatgcatc tcgcccatcg
     1021 cggccggcga gccgtgacat tcaacaatcc gggcaattgc gacatcgacc attccgagtc
     1081 cctcatctcc tgcttacccg atcacgcgat agagtggtcc gcagatattg tctctcttca
     1141 ggcaaattgc cgcgctggca atttcccgtt ctggcgcgcc gattgggtgc ggtagagttt
     1201 cgccgtagtg gcggttctga caaaggggcg gacgttccat ggcggcgaag cgagacaagg
     1261 taatcatttc ctgcgcggtg accggcgccg tccacgttcc ctcccagagc gattatctcc
     1321 cgatcacccc ggaacagatc gccgaaaacg cgatcggcgc ggccgaagcc ggcgccgcca
     1381 tcctgcacct gcatgcgcgc gatccccaga ccggccggcc gacgccgaat ccggacatgt
     1441 ttatgcagtt cctccccgag atcaaggagc gaaccgacgc cgtcgtcaat atcacgaccg
     1501 gcggcgggca gggcatgacc ctggagcagc ggctggccgc ggccatgcgg gccaagccgg
     1561 agatgtgctc actcaac
//
LOCUS       DATAOZ010000341         9359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5664940, whole genome shotgun sequence.
ACCESSION   DATAOZ010000341 DATAOZ010000000
VERSION     DATAOZ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5664940"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VHR64_08740"
     CDS             complement(<1..291)
                     /locus_tag="VHR64_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3302953.1"
                     /translation="MRFHGRVAIVTGSARGIGRAIADRLAGEGAHVLIADIDEAAAKT
                     AAAELGDMGDAYPLNVTSADSWQAALLAANNRFGPVDILVNNAGIAGRSAPTW"
     gene            complement(369..932)
                     /locus_tag="VHR64_08745"
     CDS             complement(369..932)
                     /locus_tag="VHR64_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3302954.1"
                     /translation="MNRFHALARRMRPVHASWQEAVADLTLEQVNHHERAGVLPIAFS
                     LMHLVNTEDFRSCEFLGKRPSLWERECWADKVGNTVPAVFRGTPMEVAESLRFGDVDA
                     WRAYQSAVFAQTTELLMAMDDARWDDIVMPEIPESQRRGFMYLLVGDGPVTLGDRLEV
                     VLYHHGIRHLGELEHARALVGLHGVGG"
     gene            complement(982..3189)
                     /locus_tag="VHR64_08750"
     CDS             complement(982..3189)
                     /locus_tag="VHR64_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX3302955.1"
                     /translation="MRATRRQGGRSRWAAVSCCALMLLAAMIGPVAFGQFTPNAQAAV
                     LLSPTDPMLATPRGTPEKVVRFAQEKQSTRITDTRAYIYEVYRLAPLVGLDPALIIAQ
                     SALETANWTSYYWTNFLNPAGIGIGYDGAPSYTWATGEEAARSQVVRLYIYSAGAIDP
                     GNPLYQYRDVGPTWQRVVALGYNGQVHVLDDLTGKWATDPQYGQKVASRGNGVYAVDP
                     GDPTEPAVNPVSASSSAGGDPYRTRDGDLASSFAVSGIDAPPPGLFVDYDFGQPVAFQ
                     SVQWLFRSANYADSYQLQTSSDGLTWTTRSSYVNPIAYAWNIYGGAVTARYVRMLILN
                     PTGDMRLGYLSEIEFYGHYANSVPTATSTPFPIPTATNTPTATNTPTVTNTPTVTNTP
                     AATATPTSAATIEPTATPAMLTGQLLPIVGSGGSGSGNWSGYVRDGNVRTTWQTTSDT
                     PPSQAQVYVDLGKSGSLTGVEFMFRRISGARSYSIRVSDDKKSWTTVATFNYADPLVW
                     QRAEFSASGRYVQFLFINTNGTASLGYLAEVKVYGSAASFKSAGASSAANTPTVAPIA
                     TATATAAPAFTSTPMEIIDPTVTPTPVSFGAATPVVVDESLPIVELRRTANSTETSAL
                     VDQDPVTIWHSTGEAESMIYAVADLGETQDVGSVRWLPGPDGIAGHLHVEISADGVTW
                     EGVAEPPSGLPGEWQEVAIDRTASAVRIVVTNPDHAQSIGGIAELRIEPSAEP"
     gene            complement(3345..4586)
                     /gene="fabF"
                     /locus_tag="VHR64_08755"
     CDS             complement(3345..4586)
                     /gene="fabF"
                     /locus_tag="VHR64_08755"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX3302956.1"
                     /translation="MTKRIVVTGVGAVTPLGIGAKATWEGLLAGRSGIRKIQHWDPSS
                     LEVQIAGEVDGFVAKDHMDFKLAKRMDRFAQFGVAAAREAIEHAGLTIDESNADRVAV
                     VVNTGGGGIPTIERDVNLMHDRGQKYVSPLLIPLFAPNMASCQVSMAFGIHGPSITSA
                     AACASGIQAFIDAYHMFQRDEADVILTGGTESGITPVAVAGLANMQALSKRNDDPAGA
                     SRPFDAERDGFVFSEGSAVFVLETEEHALARGANIIAEVHGGAYTSDAYHITAPHPEG
                     KGAAAAMTRAIKRSGFEPTDVDYVAAHATATTIGDLAETSALKKVFGDHAYKLAISAN
                     KSMIGHLLGAAGAVSGLGCVMAIRDNVVHPTINLHTPDPRCDLDYVPNVKREMQVNVA
                     LANGFGFGGQNAVAVFSKYQG"
     gene            complement(4684..5637)
                     /gene="fabD"
                     /locus_tag="VHR64_08760"
     CDS             complement(4684..5637)
                     /gene="fabD"
                     /locus_tag="VHR64_08760"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX3302957.1"
                     /translation="MAEWQSQPYALVFPGQGAQFVGMGAALIEASPIAAQTVREADEI
                     LGVALSDLMANGPAETLEDTYNAQPAILTISVAALRAVKTALGEELHPVMVAGHSLGE
                     FSALVAAGVLDFPDSLRLVRERGRIMKAAGETNPGAMAAVLGLDDDVLAKICRDASNE
                     SGVVVVANRNCPGQTVISGEVGALERASELAKERGAKRVARLGVSIASHSPLMANAAA
                     EFGQRVSELTLNDPQVPVVANGSAQPMTGREDVTAEISAQMAAPVDWTGSVLTMQAAG
                     IRRFIELGPGAVIAGLIKRIDREAAILGISDLGLGLPSTAK"
     gene            5782..6900
                     /locus_tag="VHR64_08765"
     CDS             5782..6900
                     /locus_tag="VHR64_08765"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3302958.1"
                     /translation="MADIYDELGVRKIINCATTYTRLGGSTMAPEVAQAVADASQAFV
                     DILELVEAVGKRLAAVTNNEAAYVSNGAAAGLSLATAAAIAGDDPALIARLPTDMDGL
                     KSEVVVHRMHRIWYDQAVRNAGARLIEIGHSYETQTWELDAAINERTAAVLYCAGGHL
                     ARNTLPLEYVIERAHARGVPVIVDAAAQVPPVTNLWHFTRDLGADVAIFSGGKNLRGP
                     QNSGLVVGTEEMIRKIRFNGPPNQRFGRSLKVGKETMIGLLKAVERYVTLDHDALWQE
                     WSDVVDKWMAAWSDCGTVTRSNTNEAGEPIPRVIMRFPSLAARDEAVAGLFGGDPPIS
                     VVLNDPTSIAFSPHQLLPGEAEVVEQTVTRKLLKPVQV"
     gene            7032..7625
                     /locus_tag="VHR64_08770"
     CDS             7032..7625
                     /locus_tag="VHR64_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302959.1"
                     /translation="MRTRAMALIAGLTIVAFLAALLGSQPQASARLQTGGTPEASPVE
                     NGTPVAGETPTETPVDSGIVTIVMWYQQNETGEFLRLFPAASTDGVTYTRGKIENDTQ
                     GGRVVFEEARNEGYPRIRVGQTDYFDAYPIYPGDPSSVQRWYYFDDDSTVRPATMVMQ
                     IVGIRGTYEDWNGTATFISRGINQGGIMIIAIRPPKQ"
     gene            complement(7672..8625)
                     /locus_tag="VHR64_08775"
     CDS             complement(7672..8625)
                     /locus_tag="VHR64_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX3302960.1"
                     /translation="MPNGMADTRAFLRSMGLPEGDAHDLPSSGKRFPDGGQWRIEIPS
                     VEGPNALQAVYDTADALDVPIHRVSQGSGVMLLTDDELDRMAQLGRNRNIEISLFVGP
                     RAGWDTGAMATSSAGRVVAPKVRGMDQLVQAVEDVRRSCEHGINSVLITDEGLLWVVG
                     AMKKAGELPSDLIIKGSVMLGASNPASIRLLEERGMTTFNVPTDLSLAQLAAIRSVVD
                     MPLDIYIEVPDDIGGFIRHYEIAEIVRVCAPVYLKFGLRNAPNIYPSGTHLEATAIAL
                     TKERVRRAKLGVDLLHRLDPDTVMSPLPCGGEQASRRAGEQ"
     gene            complement(8692..9117)
                     /locus_tag="VHR64_08780"
     CDS             complement(8692..9117)
                     /locus_tag="VHR64_08780"
                     /inference="COORDINATES: protein motif:HMM:NF022279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2568 domain-containing protein"
                     /protein_id="HEX3302961.1"
                     /translation="MLINRTPRPRAVVPSGYPPIPVIHLAIRFALEIAMLAGIWMAAE
                     YHWGDVAAILAVLAAMALWGVFGTPGDGSRGAPVVPTPGQLRLLLEIALFGIAAYGYW
                     VEWSRAASETFLTISVLHYVITWERQWWLIRGGPLPESV"
     gene            complement(9107..>9359)
                     /locus_tag="VHR64_08785"
     CDS             complement(9107..>9359)
                     /locus_tag="VHR64_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEX3302962.1"
                     /translation="PVTSIKGAIGHCMGAAGSIESVIAILSMANQTIPPTLNYATPDA
                     EIGLDIVHGAPRKADFAVSTKHSFGLGGQNACLVFGRVD"
BASE COUNT         1669 a         3094 c         2958 g         1638 t
ORIGIN      
        1 ccaggttggc gcagaccgcc cagcgattcc ggcgttgttg acgaggatat cgaccggccc
       61 gaagcgattg ttggcggcca ggagggcggc ttgccaggaa tcggccgacg tgacattgag
      121 cggataggca tcgcccatat cgccaagctc ggccgctgcc gttttggccg ccgcctcgtc
      181 gatgtccgcg atcagcacat gggcgccctc gccggccagg cgatcggcaa tcgctcgccc
      241 gatgccacgc gccgatccag tgacgattgc cactcgtcca tgaaaccgca tcgagtcctg
      301 tcctttcgtc tcgcttgttt ccatggcgtc atcttgccat acgcgagaaa gaccggcctc
      361 gatcctgttt acccgccgac gccgtgcagc ccgaccaatg cgcgggcgtg ctcgagctcg
      421 ccgaggtgac ggatgccgtg gtgatagagg acaacctcga gccgatcgcc aagcgtgacc
      481 ggtccatccc caaccagcag gtacatgaag ccacggcgct gcgactccgg aatctcaggc
      541 atgacgatgt cgtcccagcg cgcatcgtcc atcgccatca acagttcggt cgtctgggca
      601 aagacggctg actggtaggc gcgccaggca tcgacatcac cgaaccgcag cgactcggca
      661 acctccatcg gcgtgccccg gaacaccgcc ggaaccgtgt tgccgacctt gtccgcccag
      721 cattcgcgct cccagagcga tggccgttta ccgaggaact cgcagctccg gaagtcctcg
      781 gtgttgacca ggtgcatgag ggagaaggcg atcggcagaa cgcctgcgcg ttcgtggtgg
      841 ttgacctgtt cgagagtcag atcggcgacc gcctcctgcc acgaggcatg aacgggtcgc
      901 attcggcggg ccaacgcgtg aaatcgattc atggcaaatc ctggggtact ctgcgagacc
      961 cgggactgcc cacgcagatc cctacggttc cgcactcggc tcgatacgca attctgcaat
     1021 gccgccgata gattgcgcat ggtcggggtt cgtgaccacg atccgaaccg cgctcgccgt
     1081 tcgatcgatg gcaacctcct gccattcgcc cggcaacccg gacggcggct cggccacgcc
     1141 ttcccaggtc acgccgtccg ccgaaatctc gacgtgcaga tggccggcga tgccgtccgg
     1201 gcccgggagc catcgaacgg agccgacgtc ttgcgtctca cccaggtcgg cgacggcata
     1261 gatcatcgac tccgcctccc cggttgaatg ccagatcgtg accggatcct gatcgaccag
     1321 ggccgatgtc tcggtcgagt tcgccgttcg tcgcagctcg acgatcggca gggactcgtc
     1381 gaccacgacc ggcgttgcgg ctccgaatga gaccggggtg ggcgttacag tcggatcgat
     1441 gatctccatc ggcgtggagg tgaatgccgg cgccgcggtc gcggtcgccg tcgcgattgg
     1501 agcgactgta ggcgtgttcg ctgccgacga agcacccgcg ctcttgaagc tcgccgccga
     1561 gccgtacacc ttgacttcgg ccaggtagcc gagcgaggcc gtgccattcg tgttgatgaa
     1621 caggaactgc acataccggc cggaggccga gaattcggcc cgctgccaaa cgagcggatc
     1681 agcatagttg aacgtggcga ccgtggtcca actcttcttg tcgtcggaga cccgtatcga
     1741 gtacgagcga gcgccgctga tgcgacggaa catgaactcg accccggtca acgagccgct
     1801 cttgccaagg tcgacgtaga cctgcgcctg cgacggcggc gtgtccgatg tcgtctgcca
     1861 cgtcgtacgc acattgccgt cgcggacgta accagaccag ttcccactgc ctgatccgcc
     1921 actgccgacg attggcagca attgaccggt cagcatcgcc ggggtcgcgg tcggctcgat
     1981 cgttgccgct gatgtcggag tcgcggtggc cgcgggagtg ttcgtgaccg tcggtgtgtt
     2041 tgtcacggtc ggagtgttgg ttgcggtcgg cgtattcgtg gcggtcggaa tcgggaatgg
     2101 cgtcgatgtc gcggtcggga cgctgttcgc gtaatgcccg tagaactcga tttccgagag
     2161 atacccgagc cgcatgtcgc cagtcggatt caagatcagc atccgaacat accgagctgt
     2221 tacggctccc ccgtagatat tccacgcgta cgcgatcggg ttgacatagg agctgcgcgt
     2281 agtccaggtg aggccatcgc tcgaggtctg gagctgatac gagtcggcgt agtttgccga
     2341 tcggaagagc cactggacgc tctggaacgc aaccggttgg ccgaagtcgt agtcgacgaa
     2401 gagaccgggt ggcggcgcgt cgatcccgct cacggcaaag ctggaggcca gatcgccatc
     2461 gcgggtgcgg tacggatcgc cgcctgccga tgagctcgca ctgaccgggt tgaccgccgg
     2521 ctcggtcgga tcgcctggat cgacggcata gacgccgttc ccccggctgg cgaccttctg
     2581 cccatattgt gggtcggtgg cccatttgcc ggtcagatcg tcgagtacgt gaacctggcc
     2641 gttgtatccg agagcaacga cgcgctgcca ggtcgggcca acatcccggt actggtacag
     2701 cgggttgccg ggatcgatcg ccccggccga gtagatgtag agccggacaa cctgggaacg
     2761 ggcggcctcc tcgccggtcg cccaggtgta ggatggcgcg ccatcgtatc cgatgccgat
     2821 cccggccgga ttcaggaagt tcgtccaata gtaggaggtc cagttcgctg tctcgagggc
     2881 cgactgcgcg atgatcagtg ccgggtcgag gccgaccagc ggcgccaggc ggtagacctc
     2941 atagatgtag gcgcgggtgt cggtgatccg ggtcgattgc ttctcctggg cgaagcgcac
     3001 caccttctca ggcgttccgc gcggggtcgc gagcattgga tcggtcgggg agagcagcac
     3061 ggccgcctga gcattgggtg tgaactggcc gaacgcgacc gggccgatca tcgcggccag
     3121 cagcatcagg gcgcaacatg acaccgccgc ccatcgtgat cgcccgccct gtcgtcgtgt
     3181 tgcccgcatg ttgcgtccct cccgcgcccc gcacatcgcg cggcacatat ggaatctctt
     3241 cagctgagcg gggacgtggg ggatttggga tcagacgctg ttccgcgcct gctggttgcg
     3301 aaatcagtat accggaggag ccgtcacgac ccctccggtg agcgctatcc ctggtacttc
     3361 gagaagacgg cgaccgcgtt ctggccgccg aagccgaagc cattcgccag cgcgacgttg
     3421 acctgcatct cgcgcttgac gttggggaca tagtccagat cgcacctcgg atccggggtg
     3481 tggagattga tcgtcgggtg cacgacgttg tcgcggatcg ccattacgca gccgagaccg
     3541 gagaccgcgc cggccgcgcc cagcagatgc ccgatcatcg acttgttggc cgagatcgcc
     3601 aacttgtacg cgtggtcgcc gaaaaccttc ttgagcgcgc tggtctcggc gaggtcgccg
     3661 atcgtcgtcg ccgtcgcatg cgcggccaca taatcgacgt cggtcggctc gaaaccggat
     3721 cgcttgatgg cccgagtcat tgctgcggcc gcgcccttgc cttccggatg cggcgcggta
     3781 atgtgatagg cgtcagacgt gtaggcaccg ccatggacct cggcgatgat gttggctccc
     3841 cgggcgagcg catgctcctc ggtttcgagc acgaagacgg ccgagccttc cgagaagacg
     3901 aacccgtcgc gctcggcgtc gaatggccgg ctcgcccccg ccggatcgtc gttccgcttc
     3961 gagagcgcct gcatgtttgc gagtccagcg accgcgaccg gcgtgatgcc cgattcggtg
     4021 ccgccggtca gaatgacgtc agcctcgtcg cgctggaaca tgtggtacgc gtcgatgaag
     4081 gcctgaatgc cggacgcaca ggccgcggcc gacgtgatcg acggtccgtg gatcccgaac
     4141 gccatcgaca cctggcagct cgccatattc ggagcgaaaa gcgggatcag gaggggactg
     4201 acatactttt gcccgcggtc atgcatcagg ttgacgtcgc gctcaatcgt cggaatccct
     4261 ccgccgccgg tgttgaccac gaccgcgaca cggtcggcat tggactcgtc gatcgtcagg
     4321 ccggcgtgct cgatcgcttc ccgcgcggcc gccacaccga actgcgcgaa gcgatccatg
     4381 cgcttcgcca gcttgaaatc catgtggtcc ttggcgacga agccatcgac ctcgcccgca
     4441 atctgaactt cgaggctgga ggggtcccag tgctgaatct tgcgtatgcc ggatcgccca
     4501 gccagcagcc cttcccacgt cgctttggcg cctatgccaa gcggggtgac cgcgccaacc
     4561 cccgtcacca caatacgctt tgtcacgaca atctcccgtc gtatatccaa acggcggtgt
     4621 tcatgccctg cgcgctggat cgtttgttgc gccgcatagt gtactcaagt gcgcgaaata
     4681 gcttcatttt gcggtggacg gcaggcccag tccgaggtcg gagatgccca gaatcgccgc
     4741 ctcccggtcg attcgcttga tcaacccggc gatcaccgct cccgggccaa gctcgatgaa
     4801 cctgcgaatc ccggccgcct gcatcgtcag cacgctcccg gtccaatcga ctggcgcggc
     4861 catctgcgcc gagatctcgg ccgtgacgtc ctcccggccg gtcatcggtt gcgccgagcc
     4921 gttcgccacc accgggacct ggggatcgtt gagtgtcagc tcagacacac gctgcccgaa
     4981 ctcggccgcc gcattggcca tgagcggcga atgagacgcg atgctcaccc cgagccgggc
     5041 aacccgcttc gcgccgcgct ccttggccag ctcgctggcc cgttcgagcg cgccgacctc
     5101 gccggagatg accgtctgcc cggggcagtt tcggttcgcc acgacgacca cgccgctttc
     5161 attggaagcg tcgcgacaaa tcttggccag cacgtcgtca tcaagtccga gcacggcggc
     5221 catcgcgccg ggattggtct cgccggccgc cttcatgatt cgcccgcgct cccgcacgag
     5281 ccgcagggag tccgggaaat cgagcacgcc ggccgcaacc agcgcactga actcgcccag
     5341 ggagtgaccg gcgaccatca ccgggtgcaa ctcctcgccg agcgcagtct tgacggccct
     5401 cagcgccgcc acgctgatcg taaggatcgc cggctgcgcg ttgtaggtgt cttccagcgt
     5461 ctcagccggg ccgtttgcca tgagatccga tagcgcgacg ccgagaatct cgtccgcctc
     5521 gcgcacggtc tgcgcggcga tcggcgatgc ctcgatcaac gccgcgccca ttccgacgaa
     5581 ctgcgcgccc tgtcccggaa agacgagcgc gtacggctgg ctttgccact cggccatgat
     5641 gatcccccaa ctccgccggc gcccgattag ttcgcgccgt aaacgcaacc gcgaaatata
     5701 gcagacgtcc cgctccgcca atctctgcga tctggttaga tacctgaaac ggaccagcat
     5761 ggcttcggga aggacacatt cgtggcggat atctacgacg agctcggcgt acgcaagatc
     5821 atcaactgcg ccaccaccta cacgcgactc ggcggctcga cgatggctcc cgaagtcgcc
     5881 caggccgtgg ccgatgcttc gcaagccttc gtcgacatcc tcgaactcgt cgaggcggtc
     5941 gggaagcggt tggcagccgt gaccaacaat gaagccgcct acgtcagcaa tggcgcggcg
     6001 gccgggctca gcctcgccac tgccgccgcg atcgccggcg acgatccggc cctgatcgcg
     6061 cgcctcccta ccgacatgga cggtctcaag tcggaggtcg tcgtccaccg catgcaccgg
     6121 atctggtacg accaggcggt gcgcaatgcc ggcgcgcgtt tgatcgagat agggcactcc
     6181 tacgagacgc agacgtggga gctggatgcc gccatcaatg agcggacggc agctgtcctc
     6241 tactgcgccg gcggtcacct cgcccggaac acgctgccgc tcgaatacgt catcgagcgg
     6301 gcccacgccc ggggcgtgcc ggtcattgtc gatgccgccg cacaggttcc gccggttacc
     6361 aatctctggc acttcacgcg cgatctcggc gccgatgtcg ccatcttcag cggtggcaag
     6421 aacctgcggg gaccgcagaa cagcggcctc gtggtcggaa ccgaggagat gatccggaag
     6481 atccgcttca acggaccgcc gaaccagcgc ttcggccgct ccctcaaggt cggcaaggag
     6541 acgatgatcg gcctgctcaa ggcagtcgag cgctatgtca cgctcgacca tgacgcgctc
     6601 tggcaggaat ggagcgacgt ggtcgacaag tggatggccg cctggagcga ttgcggcact
     6661 gtgacacgat ccaacaccaa tgaggccggc gagccgatcc cgcgggtgat catgcgcttc
     6721 ccgtcgctcg cggcgcggga cgaggccgtg gctgggcttt ttggcggcga tccaccgatt
     6781 tccgttgtgc tcaatgaccc caccagcatc gccttcagcc cgcaccagct tttgcccggc
     6841 gaagcggagg tcgttgaaca aactgtcacg cggaaactcc tcaaacctgt gcaagtctag
     6901 tcagcctctg gtaggatgcg acgaagtccc gcagaacctc agccggtact cccgcgttat
     6961 ggtggtgaaa catccggacc tgaacgtcag tggggcatac cttcagactg gcgagaatgt
     7021 gaggcgaggc aatgcgtacc cgagcaatgg cgctgatcgc gggcctgacc atcgtcgcgt
     7081 ttctggcggc gcttctcgga tcccaaccac aggccagcgc gcgactgcag accggcggta
     7141 cacccgaggc gtcgcccgtc gagaacggca cgccggtcgc cggggagacg ccgactgaga
     7201 cgccggttga ttccggcatc gttacgatcg tgatgtggta tcagcagaat gagaccggcg
     7261 agttcttgcg gctcttcccg gccgcgtcca cggacggcgt cacctatacc cgtggcaaga
     7321 ttgaaaacga tacgcaaggt ggccgagtcg tgtttgagga ggcgcgaaac gaggggtacc
     7381 cccgcattcg cgtcgggcag accgactatt ttgacgccta tccgatctat cccggcgacc
     7441 cgtcgagtgt ccagcgctgg tactacttcg atgatgattc gaccgtgcgc cccgcgacga
     7501 tggttatgca aatcgtcggc atccggggga cgtacgaaga ctggaacggc accgccacct
     7561 tcatctcgcg cggcatcaat cagggcggca tcatgatcat cgccatccgc ccacccaagc
     7621 aatagatcaa tttgaatcgc cctgacccgc aagcgggaaa gggcgattct ttcactgttc
     7681 gcctgctcgc ctgctcgcct gctcgcctcc acacgggagc ggcgacatca ccgtatccgg
     7741 gtccaggcga tggagcaaat cgacgcccag cttcgcccgc cgcactcgct ctttggtcag
     7801 cgcgatcgcg gtcgcttcga ggtgcgtgcc cgagggataa atattcggcg cgtttcggag
     7861 cccgaacttg aggtagaccg gcgcgcagac ccgcacgatc tcggcaatct cgtaatgacg
     7921 aatgaagccg ccgatgtcgt ccggcacctc gatgtagata tcgagtggca tgtcgacgac
     7981 cgaccgaatc gcggccagct gcgccagcga gagatcggtc gggacattga acgtcgtcat
     8041 gccacgctct tcgaggagcc tgatcgaagc ggggttcgat gctccaagca tgaccgaacc
     8101 cttgatgatc aggtcggacg gcaactcgcc cgccttcttc atcgcaccga ccacccagag
     8161 caacccttcg tccgtgatca gcacgctgtt gatcccgtgc tcgcacgatc gccgcacgtc
     8221 ctcgaccgcc tgcacgagct ggtccatccc gcgcaccttc ggcgcgacca cccggccggc
     8281 cgacgaggtc gccatcgccc cggtatccca gccggcgcgc ggtccgacaa agaggctgat
     8341 ctcgatgttt cggttccggc ccagttgggc catccggtcg agctcgtcgt ctgttagcag
     8401 catcacgccg ctgccctgac tgacccggtg gatcgggaca tcgagcgcgt cggccgtgtc
     8461 atagacggcc tgcaacgcgt tcggtccctc gaccgacgga atctcgattc gccactgccc
     8521 gccatccggg aagcgcttcc cggacgacgg tagatcgtgc gcgtcgccct cgggcaaacc
     8581 catcgaacgc aggaaggcgc gggtatcagc catgccgttc ggcatcggga acctccagtg
     8641 gctagggtcg atggacgacc ggaactcgtt ctggcgtcat cctagccgag gttagacgga
     8701 ctccgggagc ggtccgcccc ggatcaacca ccactgccgc tcccacgtga tcacgtagtg
     8761 cagcaccgag atggtcagga acgtttccga cgccgcccgg gaccactcga cccagtagcc
     8821 gtaggcggcg atcccaaaga gcgcgatttc gagcaggaga cggagctggc cgggagttgg
     8881 cacgaccgga gcgccccggg agccgtcacc cggagtgccg aagacgcccc agagggccat
     8941 cgcagccagg acggccaaga tcgccgcgac gtctccccag tggtattcgg cggccatcca
     9001 gatcccggcc agcatcgcga tctcgagggc aaatcgaatc gcgaggtgga tgacagggat
     9061 tggcgggtag cctgatggaa cgaccgcgcg tggtcggggg gtgcggttaa tcaacacgtc
     9121 caaacacgag acaggcgttc tgcccgccca gcccgaacga gtgcttggtc gaaaccgcga
     9181 aatcggcctt gcgcggcgcg ccatgcacga tgtcgagacc aatctccgcg tccggcgtcg
     9241 cgtaattgag cgtcggcgga atcgtctggt tcgccatcga aaggatcgcg atcaccgatt
     9301 cgatcgagcc ggccgcgccc atgcagtggc cgatcgcgcc cttgatcgaa gtcaccggg
//
LOCUS       DATAOZ010000342         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6454266, whole genome shotgun sequence.
ACCESSION   DATAOZ010000342 DATAOZ010000000
VERSION     DATAOZ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6454266"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..546)
                     /locus_tag="VHR64_08790"
     CDS             complement(82..546)
                     /locus_tag="VHR64_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3302963.1"
                     /translation="MDVNEDLARRVQVLEDIEAIKQLKARYADACDRQYDSDTLASLF
                     TEDAIWDGGTFGRYEGREAIRQFFAGVSSDIPFAMHYMINPIIEVNGDRATGLWHLFQ
                     ACTFAGDRPIWGAARYNEEYARVDGKWLFRRLNLNSSFWTPYELGWVERQFV"
     gene            complement(572..>2020)
                     /locus_tag="VHR64_08795"
     CDS             complement(572..>2020)
                     /locus_tag="VHR64_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX3302964.1"
                     /translation="RMLVSNMRLADERLGDLRAQAEATRIGEAYLRRLIDKYGVDTVV
                     TAMAECQNYVERLTRARIKELPQGTWETDDFLDIDPDKGEGLVRIHVKLSIEEDDLHY
                     DLSGSDPAVGCFLNASYGSGFSAVVAGTKTFFPDVPLNSGFYRVVKAHLPEGSVVNAP
                     WPYAVTGFCSGAYEKIMNATFELWSRVLPERALACSFNLEYLLVGGWDRRPGYNRQFM
                     WYDWMVGGWGGRNGKDGSTATAPVFGVGLMIQPLEGQERLTPVVTTHHAILRDSAGPG
                     KYRGGCGVMKGGILSQNERSVMSYSCDRERSVTFGIEGGLPGYPHGTWLNPRGPQAQF
                     LGAIFSGLPVKEGDEFDRPSSGGGGFGDPLQRDPAQVLEDVIDDYVSVERARKDYGVV
                     INAIDPEQLVFEIDAEATEREREHIRANRRAWLEEDPESVAARYRAGELDLLDTIRRY
                     GVIVDQRDGRVLLKTTSQFREMLQHRTAAHWV"
BASE COUNT          340 a          641 c          660 g          379 t
ORIGIN      
        1 cttgccgact cgccgtcttg ccgtctcact ggcaactcac gtcttacgac ttcgaactca
       61 agggccgata gccgatagcc gttacacgaa ttgacgttcg acccagccca gctcgtaggg
      121 agtccagaag gaggagttga ggttcaggcg gcggaacagc cacttgccat cgacgcgagc
      181 gtactcctcg ttgtacctcg ctgcgcccca tatcggtcga tctccggcga aggtgcaggc
      241 ctggaagagg tgccagagac cggtcgcccg gtcgccatta acctcgatga tggggttgat
      301 catgtagtgc atggcgaagg ggatgtcgct cgagacaccg gcgaagaact ggcggatggc
      361 ctccctcccc tcgtagcgcc cgaaggtccc gccgtcccag atggcgtcct cggtgaagag
      421 ggacgccagc gtgtcggagt cgtactgccg gtcgcaggca tcggcgtagc gcgccttgag
      481 ctgcttgatc gcttcgatat cctccaatac ctggactcgc cgtgccaaat cctcgttcac
      541 gtccatgggc tctcctcgct cgttagcgtc atcacaccca gtgtgccgcc gtgcggtgct
      601 gcagcatctc gcggaattgg ctggttgtct tcagtaagac ccgcccatcc cgctgatcga
      661 cgatcacccc gtagcgacgg atggtgtcga ggaggtcgag ctcgccggca cggtagcgtg
      721 ccgccaccga ttccgggtcc tcttctagcc aggcgcggcg attggcccgg atgtgttctc
      781 gctcgcgctc tgtcgcttcg gcgtcgatct cgaagaccag ttgctcgggg tcgatggcgt
      841 tgatgaccac cccgtagtct ttgcgggcgc gctcgaccga gacgtagtcg tcgatgacgt
      901 cctccagcac ctgcgccgga tcgcgctgca gcgggtcgcc gaagccgccg ccacccgacg
      961 aggggcgatc gaactcatca ccttctttga ccgggaggcc ggagaagatg gcgcccagga
     1021 attgcgcttg tggtccgcgt gggttgagcc aggtgccgtg cgggtacccg ggcagcccac
     1081 cctcgatgcc gaaggtgacg gatcgctcgc gatcgcacga gtaggacatc acggagcgct
     1141 cgttctggct gaggatgccg cctttcatga cgccgcagcc gccgcggtac ttgcccggtc
     1201 cagcggagtc gcgcaggatg gcatggtggg tggtgacgac tggcgtcagg cgctcctggc
     1261 cttcgagcgg ctgaatcatc agtccgacgc caaagacggg cgccgtggcg gtgctgccgt
     1321 ccttgccgtt gcggccgccc cagccgccga ccatccagtc gtaccacatg aattggcggt
     1381 tatagccggg gcggcggtcc cagccaccga cgaggaggta ctccagattg aaggagcagg
     1441 ccagagcccg ttccggcagc actcgcgacc agagctcgaa ggtcgcgttc atgatcttct
     1501 cgtaggctcc cgagcagaac ccggtcacgg cgtacggcca gggggcgttg acgacgctcc
     1561 cctcgggcag gtgggctttc acgacccgat agaaaccgga gttgaggggc acgtcgggga
     1621 agaacgtttt ggtgccggcc acgacggccg agaacccgga accataggag gcattgagga
     1681 agcaaccgac cgcgggatcg gagcccgaca ggtcgtagtg cagatcgtct tcctcgatcg
     1741 acagcttgac gtgaatgcga accagaccct cgcctttgtc ggggtcgatg tcgaggaaat
     1801 catccgtctc ccatgtcccc tgtggcagtt ccttgatgcg ggctcgggtc agtcgctcga
     1861 cgtagttctg gcactcggcc atcgccgtga cgacggtgtc gacgccgtac ttgtcgatca
     1921 ggcgacgaag ataggcctcg ccgatgcggg ttgcttcggc ttgcgctcgc aggtcgccca
     1981 ggcgctcgtc cgcgaggcgc atgttgctga ccagcatccg
//
LOCUS       DATAOZ010000343         4003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6978189, whole genome shotgun sequence.
ACCESSION   DATAOZ010000343 DATAOZ010000000
VERSION     DATAOZ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6978189"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VHR64_08800"
     CDS             <1..204
                     /locus_tag="VHR64_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX3302965.1"
                     /translation="HQHGSGTDLLDPKSRVLSDGDLFTLISKGVGDTDMPAYDIALPD
                     AERWNLVGFLRNMQANPAVTPTP"
     gene            450..1247
                     /locus_tag="VHR64_08805"
     CDS             450..1247
                     /locus_tag="VHR64_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3302966.1"
                     /translation="MSLPALEAHGIRKSFKKKRNQTVNALDDVSLTINEGEVVGLLGP
                     NGSGKSTLVRVFSTLVEPDQGTVSVFGVDALKNPRPAQRMMNRVSVEASFFKKLSAVE
                     NLLYGSKFYGVTSSEAKPRIRAILERTGFDTKRAGEPMEHLSRGMQQKIALARALLTS
                     PMLMLLDEPTTGLDPRSKKDVQDLINEIRAEHNASILICTHDMDEAEALCDRIGIIVN
                     GKIVAIDEPDKLKERFRSNGVLPSLEDVFMAATGKTIDDADQDEEDD"
     gene            1247..2086
                     /locus_tag="VHR64_08810"
     CDS             1247..2086
                     /locus_tag="VHR64_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3302967.1"
                     /translation="MQAIAAPQRKSQWMTELIGALGFVQRNYYLTKRYFLWELVWLIY
                     TTGNAMSIGFIGVAIDNQTDSNRLTTYLLIGALIWSYLSMLFDILAETVSWERWEGTI
                     EYTFMSPVSRATHLFGTSIYAIAYGIVRTMIIFLVFNIFFDLEITNANYAGALLILAI
                     CSISLIGFGMMTAVMPLISPEKGQQVVFIVGSVLLLVSGVYYPIDVLPGWMQQISRFS
                     PVTYALRGIRSTLLEGTPTSQLWSDIWPLLILGAVTVPAGLMIFSAGERFAKRTGRLK
                     RNG"
     gene            complement(2171..3241)
                     /locus_tag="VHR64_08815"
     CDS             complement(2171..3241)
                     /locus_tag="VHR64_08815"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEX3302968.1"
                     /translation="MKWYPIKLTAHVRTYTFGERLIPEMLGKTGVPDGIVAETWEISD
                     YQDTTGTVTNGELAGRTLHDITMSYPTELVGAGWSGPHFPLLAKFLDASHMLPVHLHA
                     DDETAKRVHSAPNGKSEAWHILWADENATILAGLKDDPSEQELFDAYKAQDYDRVMPR
                     YPIQAGDTIYVPGGIIHSFGPGTLIYEIQQTSDLGQNVMPADLYGKKLSEAAWDENIR
                     KTLAELKNDYLPKPNPGLAITVDQGRIRVGGVCKHFAIERWALDEAVNYDLEGRRCGL
                     LSNVGDPVRIEYGDGEVEVLSRAESCILPAALGLARIVPTGESGDLIYSYVPDRQRDI
                     IDPLRDAGYTDDEIATLGHVFD"
     gene            3361..>4003
                     /locus_tag="VHR64_08820"
     CDS             3361..>4003
                     /locus_tag="VHR64_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX3302969.1"
                     /translation="MIRSSEVVLGVDSSTQSTKVVAVDLETGAVVSEGRATHTGADTQ
                     DPTDWWSALETAVHVAVTDEMTVRGIAVAGQQHGFVTLDADGDPVRPAPLWNNISSAP
                     DAERLNAQADFAAAVGSRLVASFTITKLAHLARTSPDDLARVHSICLPHDYLNFRLTG
                     TLTTDRSEASGSGWWSPVDGVRRDLLSLAADEDFANRVKVPTVLGPQATAGTLS"
BASE COUNT          725 a         1262 c         1206 g          810 t
ORIGIN      
        1 caccagcacg gttccggcac ggaccttctc gatccgaagt cgcgcgtcct gagcgacggc
       61 gatctcttca cgctgatcag caagggtgtt ggcgacaccg acatgcccgc ctacgacatt
      121 gccctgcccg atgcggagcg ctggaatctg gtcgggttcc tccggaatat gcaggccaat
      181 ccggccgtta cgccgacacc ctagttgcgt tttgccgatc catcctgtag gatcgtccag
      241 cctgctttga ggcgccgacg catgtccgga aggaaaggaa acgtggtgag acgacgacct
      301 ggtttccccg gcggatggtc ggctgctgag cgtacggcct gggttctcct gacccattaa
      361 ctctgttccc tcaacaaccg atcacctccg cgcagccgcc tgacggttgc tggcccctgt
      421 ctgtctcatt ctcatgggag caagaagcta tgagtctgcc tgcgcttgaa gcgcatggaa
      481 ttcgcaaatc attcaagaag aagcgcaacc agaccgtcaa cgcgctcgac gacgtctcgt
      541 tgacgatcaa cgagggagaa gtcgttggcc tgcttggtcc caacggcagt ggcaagagca
      601 cgctggtccg cgtcttctcg acgctggtcg agccggacca gggcactgtg tcggttttcg
      661 gcgtcgacgc gctcaagaat ccgcgacctg cccagcgcat gatgaatcgc gtcagtgtcg
      721 aggcgagctt cttcaagaag ctttcggccg tcgagaacct gctgtacggc tcgaagttct
      781 atggcgtcac ctcgtccgag gccaagccgc ggattagagc gatcctggag cggaccggat
      841 tcgataccaa gcgcgcgggc gagccgatgg agcatctctc gcgcggcatg cagcagaaga
      901 tcgcgctggc ccgggcgctc ctgaccagcc cgatgctgat gctgctcgac gagccaacga
      961 ctggtctcga tccgcgcagc aagaaggatg tgcaggacct gatcaacgag atccgggccg
     1021 agcacaacgc ctcgatcctc atctgcacac atgacatgga cgaggccgaa gcgctctgcg
     1081 accgcatcgg catcatcgtc aacggcaaga tcgtggcgat cgatgaacca gacaaattga
     1141 aggagcgatt tcgctcgaac ggcgtgttgc cctcgctcga ggatgtcttc atggcggcca
     1201 ctggcaagac catcgacgac gccgaccagg atgaggagga cgactaatgc aggcgatcgc
     1261 ggcgccgcag cgcaaatccc agtggatgac cgagctcatc ggcgcactgg gtttcgtgca
     1321 gcgcaattac tacttgacca agcgttactt cctgtgggaa ctcgtttggc tcatctatac
     1381 gacgggtaac gcgatgagca tcggcttcat cggcgtcgcc atcgacaacc agaccgactc
     1441 caatcgtctg accacttatc tcctgatcgg cgcgctgatc tggtcgtacc tgtcgatgct
     1501 cttcgacatc ctggccgaga ccgtgagctg ggagcgatgg gaggggacga ttgaatacac
     1561 gttcatgtcg ccggtgagcc gggccacgca cctcttcggc acgagcatct acgcgattgc
     1621 gtacggaatc gtgcggacga tgatcatctt cctcgtcttc aacatcttct tcgatcttga
     1681 gatcaccaac gcgaactatg ctggcgcgtt gctgatcctg gcgatctgca gcatctcgct
     1741 gatcggcttc ggcatgatga cggcggtgat gcctctgatc tcaccggaga agggtcaaca
     1801 agtcgttttc atcgtcggtt ccgtgttgct gttggtctcc ggcgtctatt acccgatcga
     1861 tgtcttgccg ggctggatgc agcagatctc gcgcttctca ccagtcacct atgcgttgcg
     1921 cggcattcgc tcgacgctgc tcgaaggcac accgacgagc cagctttgga gcgacatctg
     1981 gccactgctg attctgggtg cggtgacggt gccggcaggt ctgatgatct tcagcgcggg
     2041 cgagcgcttc gccaagcgaa ccggccgact gaagcgaaat ggatgacgct taggttttgg
     2101 gttctaggtt ttgggttttg ggcgtggtca atgatgtctg ccccattgac ctctttccct
     2161 caaccctcaa ctaatcaaag acgtgcccga gggtggcgat ctcgtcatcg gtgtacccgg
     2221 catcgcgcag cggatcgatg atgtcgcgct gccggtcggg cacatatgag tagatgaggt
     2281 ctccgctctc gccggtcggg acaatcctgg ccagtccgag ggcggccggc aggatgcaac
     2341 tctccgcgcg agacagcacc tcaacttctc cgtcgccgta ctcgatgcgg actggatcgc
     2401 cgacgttcga gaggagcccg catcggcgac cttcgaggtc ataattgacc gcctcatcga
     2461 gcgcccaccg ctcgatcgca aaatgcttgc agaccccgcc gacccggatt cgaccctggt
     2521 cgaccgtgat cgccagtccg ggattcggct tcggcaggta gtcgttcttc agctcggcga
     2581 gcgtcttgcg aatgttctcg tcccacgccg cctcgctgag cttcttcccg tagagatcgg
     2641 ccggcatcac gttctggccg aggtcgctcg tctgctggat ctcatagatc agcgtgccgg
     2701 ggccgaacga atggattatc ccgcccggga catagatcgt gtcgccggcc tgaatcgggt
     2761 agcgtggcat gacccggtca tagtcctgcg ccttgtaggc gtcaaacagc tcctgctcgc
     2821 tcgggtcgtc cttgagtccg gccaggatcg tggcgttctc gtccgcccag aggatgtgcc
     2881 aggcctccga cttgccgttg ggcgcgctgt ggacccgctt cgccgtctca tcgtcggcat
     2941 gcaggtgcac cggcagcatg tgcgaagcat cgaggaactt cgcaagcagc gggaaatgcg
     3001 gcccgctcca ccctgccccg accagctccg tcggatagct catcgttatg tcatgcaatg
     3061 tccggccggc cagctcgccg ttcgtgacgg tgccggtcgt gtcctggtag tcgctgatct
     3121 cccaggtctc agcgacgatg ccatccggca cgccggtctt gccgagcatc tcgggaatca
     3181 gccgctcgcc gaaggtgtag gtacggacgt gcgccgtcag cttgatcggg taccacttca
     3241 tctcatttag ccttcccgcg aaccggatct cgtgcccgta ttatcccgga aatcggcgcc
     3301 gtgcggctct ggcagaatac ccgcattcgg atttgccgtg gaaccaggca gggcacgttg
     3361 atgatccgct cctccgaagt ggtgctgggc gtcgactcca gcacgcaatc gaccaaggtc
     3421 gttgccgtcg acctcgaaac cggcgccgtc gtttcggaag gacgcgcgac gcacaccggc
     3481 gccgatacgc aggatccaac cgactggtgg tccgcgctcg agacggcggt ccatgtcgcc
     3541 gtcaccgacg agatgactgt gcgtggcatc gcggttgccg gccagcagca cggcttcgtc
     3601 acgctggacg cagatggcga tccagtccga ccagccccgc tttggaacaa catttcctca
     3661 gcacccgatg ccgagcggct gaatgcacag gcggacttcg ccgcggcggt cggctcccgg
     3721 ctggtggcgt cgttcacgat caccaaactc gctcacctgg cccgcacttc gccggatgat
     3781 cttgcccgcg ttcattcgat ctgcctgccg cacgactacc tgaacttccg cttgaccggg
     3841 acgctgacga cagaccgcag cgaagcctcg ggaagtggct ggtggtcacc ggtcgacggg
     3901 gtgcggcgcg atttgctctc ccttgctgcc gatgaagatt tcgcgaaccg ggtcaaggtg
     3961 ccgactgttc tcggaccaca agcgacagcc ggcacactca gcg
//
LOCUS       DATAOZ010000344         5366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8823323, whole genome shotgun sequence.
ACCESSION   DATAOZ010000344 DATAOZ010000000
VERSION     DATAOZ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8823323"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..2300)
                     /locus_tag="VHR64_08825"
     CDS             complement(81..2300)
                     /locus_tag="VHR64_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX3302970.1"
                     /translation="MSNPDRDAVDHEPAQGSPAPASDGLPSQPGKRKPIIRNDARAAQ
                     DASVDADLTDPAHRKVKPRRPDAKVLTDLTGKQQVQGKHSGDRYVRVVRPQGEDFERA
                     GPGHLVATEEAIAARGSFSRGYTKIKRMVIGAPLSTASAAHERLTKVKALAVLSSDAL
                     SSVAYATEEILRVLLVAGGLAILDVSLPISAAIILLLIIVGISYRQTIKAYPHGGGSY
                     IVAKDNLGDGPALVAGASLLAAYTLTVAVSIAASVAALISAFPVLGTHRVSLGILFIV
                     IVTILNLRGIRESGSIFAVPTYLFLFGMFVMIAVGIARTAMNGFEPEPVPPEANELAG
                     AGALTLFVVLRAFSSGCAAMTGVEAISDGVPAFKAPEWKNARTTLTVMISILAVTFAG
                     IAFLAHQFGTYPMEASQHGYETIVSQIARHTFGGENPAYYYIQFATMAILVLGANTAY
                     SDFPRLSYFLARDRFMPRQFTFRGDRLAFTTGIVTLGVLASVVLALYAGEVEGLIPLY
                     AVGVFTSFTISQFAMFMRWQKRREPGWKAGRIINFTGATATALVTTIVAVSKFRAGAW
                     LTPVVIVLLMLMMRAIHNHYQNASRELATPTPLDPDQISHTVIVPISSVNRVARQTLA
                     YARSISDNVTAVHISDGEEEIERFREEWARLEVPIPLVIIESPYRQLVGPLLKYIDEI
                     DKQRPNDTLTIVLPEFIARHWWEHLLHNQTALRLKAALLFRPGTVVTSVPYHLERTV"
     gene            2695..5283
                     /locus_tag="VHR64_08830"
     CDS             2695..5283
                     /locus_tag="VHR64_08830"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEX3302971.1"
                     /translation="MRQQRSAQHGQRHRLVAARWLALLGLILALIPAWAPAVRAADQA
                     TINITAVADDGTTALPFARFQVIDNDGNLITTRETTPPNGTVSIDIDLTDPDLTYTVT
                     METPPACANKPDDQEVGPFSAGDSVDLTFETSFDDGCTLGGISFYAYTCPSGLDLTVD
                     DYAQYRDNCLQADNGESFTLTEKDNQQQTFHLTTGEYGIDGRAPIVGLVPGDFTASQD
                     GTDPNTTLVYCLTFDGTPNEAPAPSDTSLEKVNKDGEIKLTLKDRNRIACDFFTVSEA
                     LPGADSGNNGDTSDTSNDQQPSDNTNGSEGQVVEGNQNSPQGLAGTASIEFHVATCEA
                     GYSGTDYFNDCGGNGTDNVTFTVLGQNTATTDSATSNVPATPGFGIAVIDNLPADTYT
                     MSEDVPGDFVSTWIYCAGSPGGGQRIPTPENGTQQYDIDLADGQAVICDWFIIPDQQQ
                     VEPGILRLTKYTCAPGYGGRTFAELTADCTDATDGVAFNLSNGSGYSNDETTHNVGRI
                     RFADVVPGNGYVLTEDLPGDALDYRAAFCAPNGGDYIEYRVRSNGSIPLDPIAAGDQV
                     QCLWFTVPTNQNVGTGSVEIHKSECPAGTNSDFFATCHDNPVGGIGFDVDGPGSYANS
                     GTTGDDGHVVFTDLPSGKFTISEIAPAGYSVALYAVHCTRDGAAFPTTYDDATGLRIN
                     FTLPAGANIICDWYNVPKGAAPTPTPSGGTVTVIKRFCTDDPKDIKNFRDECALYGAG
                     AGFELKSLGTGATQTGTTGNNSQVTFTGLANGAYGLKETTGDWCKAEADHVDASGNVL
                     VVNGGNTNVYIYNCGPKNVHTLPSTGTGGAGSSLPLTGWAMALLAVALLAGGVTLKPV
                     VARRGR"
BASE COUNT         1004 a         1696 c         1782 g          884 t
ORIGIN      
        1 gagatggtcg atgcacaggg ccgatgtggt cgacgagtca aggcgggtac ggagccccgc
       61 cactacggcg tcccggccgc tcacacggtg cgttcgaggt ggtaggggac gctggtgacg
      121 acggtgccgg ggcggaagag caaggccgcc ttgagccgca acgcagtctg gttatggagc
      181 aagtgctccc accagtggcg agcgatgaat tccggcagaa cgatcgtcag cgtatcgttc
      241 gggcgctgtt tgtcgatctc gtcgatgtac ttgaggagcg gcccgaccaa ttgccggtag
      301 ggtgactcaa tgatgacgag cgggatcggc acctcgagcc gggcccactc ctcacggaac
      361 cgctcaatct cctcctcgcc gtcgctgatg tggaccgcgg tcacgttgtc ggagatggag
      421 cgggcgtagg cgagggtctg acgcgcgacg cggttgaccg acgagatcgg cacgatcacg
      481 gtgtgcgaga tctgatccgg gtcaagtggg gtcggcgtgg ccagctcgcg cgaggcattc
      541 tgatagtggt tgtggatcgc gcgcatcatc agcatgagca gcacgatcac gaccggggtt
      601 aaccaggcgc cggcccggaa cttgctgacc gcgacgatcg tggtgacgag ggcagttgcg
      661 gtcgctccgg tgaagttgat gatccggccc gccttccaac ccggctcgcg ccgcttctgc
      721 caccgcataa acatggcgaa ctggctgatc gtgaacgagg tgaagacgcc gaccgcgtag
      781 agcggaatca ggccctcgac ctcgcccgcg tagagcgcca ggacaacact ggcgagcacg
      841 ccgagcgtga cgatgcccgt cgtgaaggcc agccggtcgc cccggaaggt gaactggcgc
      901 ggcatgaagc ggtcgcgagc gaggaagtac gacaggcggg ggaagtccga ataggcggtg
      961 ttcgcgccga gcacgaggat tgccatggtc gcgaactgga tgtagtagta ggccggattc
     1021 tcaccgccga aggtatgccg ggcgatctgc gagacgatcg tctcgtagcc atgttggctg
     1081 gcttccatcg gataggtgcc gaactggtgc gcgaggaaag cgatcccggc gaatgtgacg
     1141 gccaggatcg agatcatcac cgtcagggtg gtgcgggcgt tcttccattc cggcgccttg
     1201 aaggctggta cgccatccga gatcgcctcg acaccggtca ttgccgcgca gccagagctg
     1261 aaagcgcgca ggacgacgaa gagggtcaac gcaccagctc cggccagctc gttcgcttcg
     1321 ggcgggaccg gttcgggctc gaagccgttc atcgccgtcc tggcaattcc gaccgcgatc
     1381 atgacgaaca tgccgaacag gaagaggtat gtcggcacgg cgaagatact gcccgattcg
     1441 cggataccgc gcaggttcag gatcgtcacg atcacgatga ataggatgcc caatgacacc
     1501 cggtgcgtcc ccaagaccgg gaatgcggag atcagcgcgg cgaccgaggc cgcgatcgag
     1561 accgccacgg tcagcgtgta ggccgccagc aacgacgcgc cggcgaccag tgcggggcca
     1621 tcgccgaggt tgtccttggc cacgatgtag ctgccgccgc cgtgcgggta ggccttgatc
     1681 gtctgtcggt acgagatacc gacgatgatc aacaacagga tgatcgccgc ggagatgggc
     1741 aggctgacat cgagaatcgc cagaccaccg gccacgagca aaacccgcag aatttcctca
     1801 gtcgcgtagg ccacggagga gagcgcgtcg gatgagagca cggccagcgc cttgaccttg
     1861 gtcagccgct cgtgcgcggc cgatgcggtc gagagcggcg cgccgatcac catgcgcttg
     1921 atcttcgtgt atccgcgtga gaaggatccg cgcgcggcga ttgcctcctc ggtcgcgacg
     1981 aggtgacccg ggccggcccg ctcgaagtcc tcgccctgcg gccggacgac gcggacgtaa
     2041 cgatcaccgg aatgcttgcc ctggacctgc tgcttgccag tcaggtcggt cagcactttg
     2101 gcatcgggac ggcgcggctt gaccttgcgg tgggctggat cggtcaggtc ggcgtcgact
     2161 gatgcatcct gggcggcgcg ggcgtcgttc cggatgatgg gtttgcgctt gcctggttgg
     2221 ctgggaagcc cgtcgctggc gggcgccggc gagccctggg caggctcgtg atcgaccgca
     2281 tcgcgatcag gattcgacat accgcgactc ctcgatcccg tcaggacatg attgtgcctg
     2341 gtccggaccg gcgccgaacc cggcgccgct accaatccga accaggagat gttcctggcg
     2401 gcatgacccg tggacttttc atcactaatc ccgtggccgg gggattgtag cagcctcgcc
     2461 acgcattgac acccaagttc gcctagaatt gtgcggcacc cccgaaaccc atttcccgcc
     2521 caagcgttac gtggggtgta tggctccaat gtgcgcgaac gatggccgcc acgttgggga
     2581 cggattgggg atcgcgacgg cgcggtggga cggaaatact tgtcaccgtg tgctgaagac
     2641 aggtgcatgg ccggccgggg gattcgagcc gtggtcatgg gagggtagga gatcgtgagg
     2701 cagcaacgtt cggcgcaaca tggacaacga caccggctcg tggcggcgcg ctggttagcg
     2761 ttgctcggac tgatcctggc gttgatcccg gcctgggcgc cggccgtgcg cgcggccgat
     2821 caggcgacga tcaacatcac tgcggtcgcg gatgacggta cgacggcgct ccccttcgcc
     2881 cgcttccagg tcatcgacaa tgacggcaac ctgatcacca ccagggagac cacgccgccc
     2941 aacggcacgg tctcgatcga tatcgatctg accgatcctg atctgaccta cacggtcaca
     3001 atggagacgc cgcccgcctg cgccaacaag ccggacgatc aggaggtggg cccgttctcg
     3061 gccggcgact cggtcgatct caccttcgag acctcgttcg acgacggttg caccctcggc
     3121 gggatctcct tctatgccta tacctgcccg tcaggtctcg acctgaccgt cgacgactac
     3181 gcgcagtacc gtgacaactg cctccaggcc gacaatggcg agtccttcac gctgaccgag
     3241 aaggacaacc agcagcagac atttcacctg accacgggcg agtacggcat tgatggccgg
     3301 gcgccgatcg tcggcctcgt gccgggcgac ttcacggcca gccaggacgg caccgacccg
     3361 aacacgacgc tcgtctactg cctgaccttc gacggcactc cgaacgaggc gccggctccg
     3421 tccgacacct ccctcgagaa ggtgaacaag gacggggaga tcaagctcac gctcaaggat
     3481 cgcaaccgga tcgcctgcga cttcttcacg gtctccgagg cgctgccggg tgctgactct
     3541 ggcaataacg gcgacacgtc cgacacctcg aacgatcagc agccgagcga caacaccaat
     3601 ggcagcgagg ggcaggtcgt cgagggcaac cagaatagcc cgcagggcct ggccggcacc
     3661 gccagcatcg agttccatgt cgcgacctgc gaggccggtt acagcggcac cgactatttc
     3721 aacgactgcg gcgggaacgg cacggacaat gtcaccttca ccgtgctcgg ccagaacacg
     3781 gctacgaccg attcggcgac atccaatgtt ccggccacgc ccggattcgg catcgcagtg
     3841 atcgacaacc tgccggccga cacctacacg atgagcgagg atgtgcccgg cgacttcgtc
     3901 tcgacctgga tctactgtgc cggcagcccg ggcggcggac agcgcatccc gacgccggag
     3961 aacggcacgc agcagtacga catcgatctt gccgacggcc aggcggtgat ctgcgactgg
     4021 ttcatcatcc cggaccagca gcaagtcgag ccgggcatcc tgcgcctgac caagtacacc
     4081 tgcgcgccgg gttacggcgg tcggaccttc gcggaactca cggccgactg cactgatgcg
     4141 accgacggcg tcgcattcaa cctcagcaat ggctccggct actccaacga cgagaccacg
     4201 cacaatgtcg gccgaattcg cttcgccgac gtggtcccgg gtaatggcta cgtgctgacc
     4261 gaggatttgc cgggcgacgc gctcgattac cgggcggcct tctgcgcccc gaacggcggc
     4321 gactacatcg agtaccgcgt gcgcagtaac ggctcgatcc cgctcgaccc aatcgccgcg
     4381 ggcgaccagg tccagtgcct ctggttcacc gtgccgacga accagaatgt cggcaccggc
     4441 tcggttgaga tccacaagtc ggaatgcccg gcggggacga acagcgactt cttcgcgacc
     4501 tgccacgaca acccggtcgg cgggatcggc ttcgacgtgg atggtccggg cagctacgcc
     4561 aacagcggca cgaccggcga tgacggccat gtggtcttca ccgacttgcc atccggcaag
     4621 ttcacgatct ccgagatcgc gccggcgggg tactcggttg ccctttatgc agtccactgc
     4681 acgcgggatg gcgcggcctt cccgacgacc tacgatgatg cgacgggcct gcggatcaac
     4741 ttcacgctgc cggccggcgc gaacatcatc tgcgactggt acaacgtgcc gaagggtgcg
     4801 gcgccgaccc cgacgccgag cggtggcacg gtcacggtca tcaagcggtt ctgcaccgat
     4861 gacccgaagg acatcaagaa cttccgcgac gagtgcgcgc tgtacggcgc cggcgcgggc
     4921 ttcgagctca agtcgctcgg cacgggcgcg acccagaccg gcacgaccgg caacaacagc
     4981 caggtgacct tcactggact cgccaatggc gcgtacgggt tgaaggagac gaccggcgac
     5041 tggtgcaagg ctgaggccga ccacgtcgat gccagcggca atgtgctggt cgtcaacggc
     5101 ggcaatacga acgtctacat ctacaactgt gggccgaaga acgtccacac gctgccgtcg
     5161 acgggtacgg gtggagcggg gtcatccttg ccgctaaccg gctgggcgat ggcgctgctg
     5221 gcggtcgcgc tgctggccgg tggcgtgaca ctgaagccgg tggtggcacg ccgcggacgg
     5281 taagtctctc gtctttggtc attggtcatc ggtctttggt ctttggtctt tggtctttcg
     5341 aagtttcggt aaggagagag gcgcct
//
LOCUS       DATAOZ010000345         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3034972, whole genome shotgun sequence.
ACCESSION   DATAOZ010000345 DATAOZ010000000
VERSION     DATAOZ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3034972"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VHR64_08835"
     CDS             <1..195
                     /locus_tag="VHR64_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302972.1"
                     /translation="GGGLGGVVSDVVGSLFGGDDKSKSTSGSGGGMGDLGAFMNSTTG
                     KMVLGGIAAYLTKELMDGRH"
     gene            complement(274..438)
                     /gene="rpmG"
                     /locus_tag="VHR64_08840"
     CDS             complement(274..438)
                     /gene="rpmG"
                     /locus_tag="VHR64_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX3302973.1"
                     /translation="MAKKKGDRIVIKLKSTESEYIYYSEKNKRNDTQRLELKKYDPLL
                     RRHVVFREAR"
     gene            656..1381
                     /locus_tag="VHR64_08845"
     CDS             656..1381
                     /locus_tag="VHR64_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3302974.1"
                     /translation="MPNAADLNSFLSLPANRGLGVDIAERLRSAILTGYFGPGERLPE
                     EQLAKTMGVSRGPVREALVTLEREGLIVIRRNRGAFVAQLSREDLDEVYSLRVAIERL
                     AVERCVANATEEDIAELQSVVDRIAELMEDGITEQDAAELDLKFHDIMYRSGRHRRLY
                     DTWATLRPQIHVLLLNRNVAHEDFRDLVVPAHQAIIDAVASRDTDLAVTITMAHLRES
                     YDRVRDARQYLPARRPAATDTGA"
     gene            1489..2286
                     /locus_tag="VHR64_08850"
     CDS             1489..2286
                     /locus_tag="VHR64_08850"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX3302975.1"
                     /translation="MKVNAAKAKMLAGGTAFGFELGFGSPLVAEALSRTGIDYLQIDT
                     QHGDWGPGSTLAALVAMEGGTATPMARVARNDYTQIGRLLDEGMMGIVVPMVHTADDA
                     KAAADACRFPPLGTRSCGWGRIGRTDPDYWDWIDQQVLLMVQIESITAVENAEAILST
                     PGVDGCWTGPADLAYSMGIHPRDQKSDDRHARALERILEACANTGKIPGIAAMNPEDA
                     ADRAAQGWRFINAGADYQLLMDAAETGLGNVGLEWLPTSARVRTATY"
BASE COUNT          427 a          791 c          727 g          402 t
ORIGIN      
        1 ggaggcggct tgggcggcgt ggtgtctgac gttgtgggca gcctgttcgg tggcgacgac
       61 aaatccaagt cgacatccgg ctcgggcggc ggaatgggcg atctcggcgc cttcatgaac
      121 agcaccaccg gcaagatggt cttgggcggg atcgccgcgt acctgaccaa ggagctgatg
      181 gacggccgac actaggtcgc ctcctccgca ttcccgaaaa gcgacgaggg ccggtcatac
      241 gaccggccct cgcttcgttc ggaagagatt cgcctagcgg gcttcgcgga agaccacgtg
      301 gcggcgcagg agcgggtcgt acttcttgag ctcgagccgc tgcgtatcat tgcgcttgtt
      361 cttctcggag taatagatgt attcgctctc ggtgctcttg agcttgatga cgatgcgatc
      421 gcccttcttc tttgccatcc cgactcagcg ctccttgcgc acacaaaaac aaaagggcgt
      481 ccaggccctc gtcaaccggt ccactatagc cgacgcccgg ccatctgacc acccggccca
      541 gagcgccccg atccgaaaat cgggccttga caggctccgg agtatttgcg agattgtcga
      601 caatcgacaa cctgcatgaa catatctcgc cctcttgtcg tcgaggacgc cgaccatgcc
      661 caacgcggcc gacctgaaca gttttctctc actgcctgcc aatcgcggtc tcggcgtcga
      721 catcgccgaa cgattgcgca gcgccattct taccgggtac ttcggcccgg gcgaacgcct
      781 cccggaagaa cagctcgcca agaccatggg cgtcagtcgc ggtcccgtcc gcgaggccct
      841 ggtcacgctc gagcgcgagg gcctgatcgt catccgccgc aaccggggcg cattcgtcgc
      901 ccagctctcc cgcgaggacc tcgacgaggt ctattcgctg cgggttgcga tcgagcggct
      961 ggccgtcgag cgctgcgttg ccaacgcgac ggaagaggac atcgccgagc tgcaaagcgt
     1021 ggtcgatcgc atcgcagaac tgatggaaga cggcatcacc gagcaggatg cggctgagct
     1081 cgatctcaag ttccatgaca tcatgtaccg ctccggacgt caccgccgtc tctacgacac
     1141 ctgggctacg ctccggccgc agatccacgt cctcctcctc aaccgaaacg tcgcccacga
     1201 ggacttccgc gacctggtcg tgcccgctca tcaggccatc atcgatgccg tcgccagccg
     1261 ggataccgat ctcgcggtca ccatcacgat ggcgcacctg agggagtcgt acgaccgtgt
     1321 tcgcgatgcc cggcaatatc tccccgcgcg gagacccgca gcaaccgaca ccggcgcttg
     1381 atcttcggct atcagcgcgc gatccagagt tcctgcacaa tgccggtcga aagatcgagc
     1441 gatccgttct ctggttgacg cggtaaccgc gcagaagacg aggattccat gaaagtcaac
     1501 gccgcgaagg ccaagatgct cgccggtggc acggcgttcg gattcgagtt gggcttcggc
     1561 tcgcccctgg tcgccgaagc gctctcccgc accggcatcg actacctcca gatcgacacc
     1621 cagcacggcg actggggtcc gggcagcacg ctcgccgcgc tggtcgcgat ggagggcggt
     1681 accgccacgc cgatggcccg ggtcgcccgc aacgactaca cgcagatcgg ccggctgctc
     1741 gacgagggca tgatgggcat cgtcgtgccc atggtccaca ctgccgatga cgcgaaggcc
     1801 gcagccgatg cctgccgctt cccgccgctc ggcacgcgct catgcggctg gggccgaatc
     1861 gggcggaccg atcccgacta ctgggactgg atcgatcagc aggtgttgct gatggtccag
     1921 atcgagagca tcaccgccgt cgagaatgcc gaggcaatcc tgtcgacccc gggcgtggac
     1981 ggctgctgga ccggcccggc cgacctcgcc tactcgatgg gcatccaccc gcgcgatcag
     2041 aagtcggacg atcgccacgc tcgcgcgctc gagcgaatcc tggaggcctg cgcgaatacc
     2101 ggcaagatcc cgggcatcgc cgcgatgaac ccggaggacg cggctgaccg cgcagcgcag
     2161 ggctggcgct tcatcaacgc cggcgccgat taccagctcc tgatggacgc cgcagagaca
     2221 ggtttaggca acgtcgggct cgagtggctg ccaacctccg cgcgagttcg cactgcaacc
     2281 tactagggat ggttcgcatt ggaaaccggc cgtagattcg gccggtttcg tcgttgccgg
     2341 acatgac
//
LOCUS       DATAOZ010000346         5803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8298537, whole genome shotgun sequence.
ACCESSION   DATAOZ010000346 DATAOZ010000000
VERSION     DATAOZ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8298537"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VHR64_08855"
     CDS             <1..869
                     /locus_tag="VHR64_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX3302976.1"
                     /translation="LAPQVLREVGLVEALSRGSDVVDYGDVEFDAAKSASRDKESGII
                     APKSLMSMIAAVRGAVARAYDEGRMPVVIGGDCALLLGGLLAARDQLGYGPALLFVDG
                     HEDSYEPRKSMNGEAADMELGLATGFTTVKGLPELADELPIVDPHQVVLIGARDSDEV
                     ETEGHKSIGTRVIVLDDTELRAAGVESTILRWLEQFQHKPGRFWFHLDWDVLSSDEMP
                     AVSYPQPGGLVWAEVETIARAALHADHLIGVDTTLYNPRLDPKRTAARRIVDFLAEVT
                     AAPEREPRLGRI"
     gene            906..1682
                     /locus_tag="VHR64_08860"
     CDS             906..1682
                     /locus_tag="VHR64_08860"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEX3302977.1"
                     /translation="MTNRSTRRMFLRMSAAGLSILAIRPLGATAADGDAAQLIQAAAK
                     AMAALKTFHFEMETIEGQSTILENLELQKVEGDVLRPDSFRATLTAKVAVVKVSVDVV
                     SVGGAVWVTDPLQAGTVWRQVAGDGEQNASAAFTDLINPDRMFLAAISLIDDPKIEGT
                     EKINGEECTVVTGQFDPKRLQELASPVAGELGTPGTAEVNTPSMLTGEPVYLSAWIAG
                     DGRVLRIEEEGPLTTSESNDVVRAITFSKFDEPIEITAPQ"
     gene            complement(1750..2385)
                     /locus_tag="VHR64_08865"
     CDS             complement(1750..2385)
                     /locus_tag="VHR64_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX3302978.1"
                     /translation="MSTNGKPVSVDFYFDFLCPWAFRASEWIRDVREQLGPDHLEINW
                     RYFPLEQVNSEEGPDWKLWEQPDEQTTRGLEMFRGALAAWQQDQGEKFDEFHRLVFRT
                     RHGEYTVDGERPSVHEIAESLGFDMDRFHKDFADRSLLSRIGEDYEFARNQLGVFGVP
                     TLVFEGDQAAYIKLMPKPEQGDAVKVWDEVSTVIADRPLIREIKRPTPPSS"
     gene            complement(2442..3044)
                     /gene="coaE"
                     /locus_tag="VHR64_08870"
     CDS             complement(2442..3044)
                     /gene="coaE"
                     /locus_tag="VHR64_08870"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX3302979.1"
                     /translation="MSRPIVIGVTGGIATGKSSVLAILREHGVETIDADTVYHQLIAP
                     GGSLVVPIANRFGRDMIAEDGSIDRRALAGIVFADREALADLDALTHPAVIAAVNERI
                     EQSDAAFVAVDAVKLIESGMADDCDSVWLVVCDPAVQRARLMARNGLTAEEADLRLAA
                     QPNEAERRAKADIVIDNSGDLPALERQVAASMHVVKQLPN"
     gene            complement(3041..4960)
                     /locus_tag="VHR64_08875"
     CDS             complement(3041..4960)
                     /locus_tag="VHR64_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX3302980.1"
                     /translation="MPATLLTVSNLTKAYGAEQIFSGLSFQLVEKEHVALVGVNGAGK
                     STVLKIIAGIEEPTSGDVAPVSGLRITYLPQEARFDSDQSIREVAQEAFADALKAAEH
                     MREIEQALGSADAEEFERLMAVYERLHAHFEVGGGYDIEHRTEEVLVGLGFEESQFDT
                     PVHLLSGGQKTRVALARALLADPDLLLLDEPTNHLDLTMLEWLEGFLRSWGGACLIVS
                     HDRYFLDRVTTRTLDLAFGRIEDYPASYARYLVLRTERRGRHQKEYQEQQAYIARTEE
                     FIRKYKAGQRSREARGRQTRLERLERIERPQDHAELAIRIQSTLRSGTQVLTSRPLTV
                     GYRSGSQDTVLVRTKDLLIERGDKVGLVGPNGAGKTTLLKTLVGEIPTLKGRFETGVN
                     VKVGYYAQAHEQLPAGGTPLSVVLDAQPMGEESARNYLGRFLFSEDDVFKPVTGLSGG
                     ERSRLALAVLLLQNANFLILDEPTNHLDIAARETLEELLGQFDGTVLFVSHDRFFTDR
                     IATKIWAVGDGQLTEYLGNYTDYQRALGRRQDPVPVKAPEPVKVEAPTPVERRPHQSA
                     GRIQKTLTGVERDIARLEGKLNELSDALAIASIEADVDALARLGTEYERVQTDLDAAY
                     ARWEELSADLEAVAQ"
     gene            5090..>5803
                     /locus_tag="VHR64_08880"
     CDS             5090..>5803
                     /locus_tag="VHR64_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX3302981.1"
                     /translation="MLWPNTTTRSERGDLVIGGCSVNDLAEKFGTPLYIFDEATLHDR
                     AERVVRAFSERNPDAGVVYGGKAYLSPAIVRILHSHGVGLDVVSGGEIYGGLRAGIPA
                     ADMVFHGNNKTADELRYALAEGVGVIVLDNEDEISRLDEIAGQVAVGLNQTGTKVPVM
                     LRLNPGVDVHTHAKISTGIVDSKFGFPVWEDNARNAVALLASCRNLALVGFHMHLGSQ
                     VSTHEAMPIAIERQVAFAAE"
BASE COUNT         1002 a         1799 c         1938 g         1064 t
ORIGIN      
        1 cgctcgcgcc ccaggtcctg cgagaggtgg gcctggttga ggcactgtcc cggggcagcg
       61 atgtcgtcga ttacggggac gtcgagttcg atgctgcaaa gtcggcctcc cgcgacaagg
      121 aatccgggat catcgcgccg aagtcgctga tgagcatgat tgcggccgtg cgaggcgccg
      181 tcgcccgcgc ctacgacgag ggtcggatgc cggttgtgat cggcggggat tgcgcgctcc
      241 tgctcggcgg gttgcttgct gctcgggatc agctcggcta cggtccggcc ctgctcttcg
      301 tcgatggcca cgaggatagc tacgagccgc gcaagtcgat gaatggcgag gcggccgata
      361 tggagctggg tctggcgact ggcttcacga cggtcaaggg cctgcccgag ctcgcggacg
      421 agctgccgat cgtcgatccc caccaggtcg tgttgatcgg cgcgcgggac agcgacgagg
      481 tcgagaccga gggccacaag agcatcggca cgcgcgtgat cgtcctcgac gacacggagc
      541 tgcgcgcggc tggcgtcgaa tcgacgatcc tgcgctggct ggagcagttt cagcataagc
      601 cgggccgatt ctggttccat ctcgactggg acgtgctgtc gtccgatgaa atgccggccg
      661 tctcctaccc gcagccaggt ggtctggttt gggccgaggt cgagacgatc gctcgcgcgg
      721 ctctccatgc cgatcacctg atcggtgtcg atacgacgct ctacaacccg cggctcgatc
      781 cgaagcgcac ggcggcgcgc aggatcgttg actttctggc cgaagtcacc gccgctccgg
      841 aacgggagcc gcgcctcgga cgtatctaga acgggaatgc agttttctgt tcaaggatgg
      901 ctctgatgac gaatcgatca acccggcgca tgtttcttcg aatgagcgcc gctggtctct
      961 caatattggc aatccgacca ctcggcgcga ccgcggcaga tggcgatgcg gcgcagctca
     1021 tacaggctgc cgcaaaggcc atggccgcgc tcaagacctt ccacttcgag atggagacca
     1081 tcgaggggca gtcgaccatc ctcgaaaacc tcgagctgca gaaagtggag ggagatgtac
     1141 ttcgccccga ttcgttccgg gccacgctga cggcgaaggt tgcggtggtg aaggtgtcgg
     1201 ttgacgtcgt gagcgtcggc ggcgccgttt gggtgaccga tccgctgcag gcaggcacgg
     1261 tctggcggca ggtggccggt gatggcgagc agaatgccag tgcggcgttt accgacctga
     1321 tcaacccgga tcggatgttc ctggccgcga tatcgctgat tgacgacccg aagatcgaag
     1381 gtaccgagaa gatcaacggc gaggagtgca ccgtcgtgac cggccaattt gatccgaagc
     1441 ggctccagga gttggccagc ccggtggcag gcgagctcgg aacgccgggc acagcagagg
     1501 tcaacacgcc atcgatgctg acgggcgaac cggtctacct ttcggcctgg attgccggtg
     1561 acgggcgggt gctgaggatc gaggaagagg gaccgctgac cacgagcgag tcgaacgacg
     1621 tggtgcgggc gatcaccttc tcgaaattcg acgaaccgat cgagatcacg gcgcctcaat
     1681 gagttgaggg ttgagagtta agagttaagg gaaattggcg cgcggccatc gagccgcgcg
     1741 ccagtctgtt tagctgctgg gcggggttgg gcgcttgatc tcgcggatca gcgggcgatc
     1801 ggcgatgacg gtcgagacct cgtcccagac cttgacagca tcgccctgct ccggcttcgg
     1861 catcagcttg atgtaggccg cctggtcgcc ctcgaagacg agtgtcggca cgccgaagac
     1921 tccgagttga ttgcgcgcga actcatagtc ctcgccgatg cgggagagaa gtgagcgatc
     1981 ggcgaagtcc ttgtggaacc gatccatgtc gaagccgagg ctctcggcaa tctcatgcac
     2041 agaaggtcgc tcgccgtcga cggtatattc gccgtggcgg gtgcggaaga cgagccggtg
     2101 gaactcgtcg aacttctccc cctgatcctg ctgccaggcg gccagcgcac cccggaacat
     2161 ctcgaggccg cgggtcgtct gctcgtccgg ctgctcccag agcttccagt ccggaccttc
     2221 ctcggagttc acctgctcga gcgggaaata gcgccaattg atctcgaggt ggtcgggacc
     2281 gagctgctct cgcacatcgc ggatccactc ggacgcgcgg aatgcccacg ggcagaggaa
     2341 atcaaaatag aagtcaaccg ataccggctt cccatttgtc gacacgaact gaaccctcca
     2401 aagcactcaa agcaactctg cttacgatgc attcaatact atcaattcgg tagttgcttt
     2461 acaacgtgca ttgatgccgc aacctgccgc tccagtgccg gaaggtctcc gctgttgtcg
     2521 atcacgatgt ccgccttggc ccggcgctcc gcctcgttgg gttgcgcggc caggcgcagg
     2581 tcggcctcct cagccgtcag gccgtttcgc gccatcaatc gcgcccgctg cacggccgga
     2641 tcgcagacga cgagccagac cgaatcgcag tcgtcggcca tgccgctctc gatcaacttg
     2701 acggcatcga ccgccacgaa cgcggcgtcg gattgctcga tgcgctcgtt gacggcggcg
     2761 atgactgccg gatgggtcag ggcgtcgaga tcggccagcg cctctcgatc ggcgaagacg
     2821 atcccggcaa gggcgcgccg gtcaatcgag ccatcctcgg cgatcatgtc acggccgaac
     2881 cggttggcga tcggcacgac gagcgagccg cccggcgcga tgagctggtg gtagacggtg
     2941 tcggcgtcga tggtttcgac accatgttcc ctgaggatgg cgagcacgct cgatttgccg
     3001 gtcgcgattc cgccggtgac gccgatgacg atcggccggc tcactgcgcg acggcctcca
     3061 gatcggccga gagttcttcc cagcgagcgt atgcggcgtc gagatcggtc tggacccgct
     3121 cgtactcggt tccgagccgg gccagcgcat cgacgtcggc ctcgatgctg gcgatcgcga
     3181 gcgcgtcgct cagctcgttg agcttgcctt cgagcctcgc gatgtctcgc tcgaccccgg
     3241 tcagcgtctt ctgaatccgc ccggccgact gatgcggtcg tcgctcgacc ggagttgggg
     3301 cctccacctt gaccggttcc ggcgccttga ccgggaccgg gtcctgccgg cggccgagcg
     3361 cgcgctggta gtcggtgtaa ttgccgaggt actcggtcag ctggccatcg ccgaccgccc
     3421 agatcttggt cgcgatccgg tcggtgaaga agcggtcatg cgagacgaac aggacggtgc
     3481 cgtcgaactg gccgagcagc tcttcgagtg tctcgcgcgc ggcgatgtcg aggtggttag
     3541 tcggctcgtc gaggatgagg aagttcgcgt tctggagcag caggacggcc agggccagcc
     3601 gcgatcgctc gccgccggag aggccggtga ccggcttgaa gacatcgtcc tcgctgaaga
     3661 ggaagcggcc gaggtagttc ctggccgact cctcccccat cggctgggca tcgagcacga
     3721 ccgaaagcgg cgtgccgccg gccgggagct gctcgtgcgc ctgcgcgtag tagccgacct
     3781 tgacgttgac gccggtctcg aagcgaccct tgagcgttgg aatctcgccg accagcgttt
     3841 tgagcagggt ggtcttgccg gcgccgttcg gaccgaccag gccgaccttg tcgccacgct
     3901 cgatcagcag gtcctttgtc cggacgagca ccgtgtcctg actgccggag cggtatccga
     3961 ccgtcaatgg ccgggaggtc agcacctggg tgccgctgcg cagggtggac tgaatgcgga
     4021 tggcgagctc tgcgtgatcc tgcggtcgct cgatccgctc caaccgctcg aggcgtgtct
     4081 gccggccccg ggcctcccgc gagcgctggc cggccttgta cttgcggatg aactcctcgg
     4141 tccgggcgat gtaggcctgt tgctcctggt attccttctg atgccgcccg cggcgctcgg
     4201 tccgaagcac caggtagcgc gcgtacgaag ccgggtagtc ctcgattcga ccgaaggcga
     4261 ggtccagcgt ccgggtcgtg acccgatcga ggaaatagcg gtcgtgcgag acgatcaggc
     4321 aggcgccgcc ccaactgcgc aggaaacctt cgagccattc gagcatcgtc agatcgagat
     4381 ggttggtcgg ctcatcgagc agcagcagat cgggatcagc cagcagtgcc cgggccagcg
     4441 cgacccgcgt cttctgacct ccgctgagca gatgcaccgg cgtgtcgaac tgcgactcct
     4501 cgaaaccgag accgaccagc acctcttcgg tccggtgctc gatgtcgtac ccgccgccga
     4561 cctcgaagtg ggcgtgaagc cgctcgtata cggccatcag ccgctcgaac tcttcggcat
     4621 cggccgagcc aagtgcctgt tcgatctcgc gcatgtgctc ggctgccttg agggcgtccg
     4681 caaatgcctc ctgcgcaacc tcacggatcg actggtcgct gtcgaagcga gcctcttgcg
     4741 gcaggtaggt gattcgcagg ccgctgaccg gtgcgacgtc accgctggtc ggctcctcaa
     4801 tcccggcgat gatcttgagc acggtcgact tgccggcgcc gttgacgccg acgagcgcga
     4861 cgtgctcttt ctcgacgagc tggaacgaca ggccgctgaa gatctgttca gcgccgtagg
     4921 ccttggtcag attcgagaca gtcagaagcg tagcgggcat gattggtgca ttatccggag
     4981 cagtcgcaat ggcgcggcag atgccgactg gaaagtatat ctgccggctg ttcatgccat
     5041 gtgccggttc acgattagac tgtgtacgta cacaacggag gatgattcgg tgctttggcc
     5101 caatacgacg acccgatctg agcgcggcga cctggtgatt ggcggctgct ccgtcaacga
     5161 tctggcggag aagttcggca cgccgctcta catattcgat gaagccacgc ttcatgaccg
     5221 ggctgaacgg gtggttcgcg ccttttccga gcgcaacccg gacgcagggg tcgtctacgg
     5281 cggcaaggca tacctctcgc cggcgatcgt ccgaattctg cacagccatg gagtcgggct
     5341 tgatgtcgtc tcgggcggcg agatctacgg cgggttgcgc gccggtatcc cggcggccga
     5401 catggtcttc cacggcaaca acaagacggc cgacgagctg aggtatgcgc tggcggaggg
     5461 cgtcggcgtg atcgtgctcg acaacgagga cgagatcagc cgcctcgacg agatcgccgg
     5521 tcaagtcgcc gtcggtctga accagaccgg taccaaggta cccgtgatgt tgcggttgaa
     5581 ccccggggtc gacgttcata ctcacgccaa gatcagcacc ggcatcgtcg actccaagtt
     5641 cggcttcccc gtctgggaag acaacgcgcg gaacgcggtt gccttgctgg cctcgtgccg
     5701 gaatctcgcg ctcgtcgggt ttcacatgca cctggggtcg caagtcagca cgcatgaggc
     5761 gatgccgatt gctatcgagc ggcaggttgc cttcgcggcc gag
//
LOCUS       DATAOZ010000347         7978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8033542, whole genome shotgun sequence.
ACCESSION   DATAOZ010000347 DATAOZ010000000
VERSION     DATAOZ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8033542"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1025)
                     /locus_tag="VHR64_08885"
     CDS             complement(219..1025)
                     /locus_tag="VHR64_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX3302982.1"
                     /translation="MRPVTEADHRDRIQRVITQVRTEPERTLSVGDMADIAALSPFHV
                     IRVFREITGYTPAAIQTALRVQEAKRLLAAEGSSVTDACYAVGFSSLGSFSQRFTTLA
                     GVKPSEYRNAVEFADSLADDLSRLQREPLMRMHERHTVSGRIIGDASRPAYYFVGLFP
                     PGPPRGRPLLGDVLEEPGNFVIRHVPDGVYAIYCAAVAHPEKSIDWVMPDTEWQTGGG
                     QSVQMICGMPVHSLEIELRPRIPVMVPLMTTVMAIPNLADDARMNHTLKI"
     gene            complement(1037..2323)
                     /locus_tag="VHR64_08890"
     CDS             complement(1037..2323)
                     /locus_tag="VHR64_08890"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HEX3302983.1"
                     /translation="MTASTPESVFTMDTSSIKYGPGATREVGDDARDRGLKRVMLFSD
                     QNLTATDGYATARDSLKAAGLDVIEYSDVRVEPTDASFSKAIEAATEAKPDGFVAFGG
                     GSVIDTAKAANLYYSYPADLLTYVNAPIGQATPVPGPLKPLIAIPTTAGTGSETTGVA
                     IFDLVEMHAKTGIAHRALRPVLGIVDPLNTLSMPAMVTASSALDVLSHAIESYTALPF
                     DQRPAPDAPKLRPAYQGANPISDIWASKAMEMVAQCLPVVLERPDDVHARGQMMLAAT
                     YAGIGFGNAGVHLPHGMSYPVAGMVRDWRPAGYAVDHPIVPHGISVILNAPAVFRFTA
                     QASPHRHLCAAQLLGADIQDNSVHDAGDILANRLIEIMRMTGMPNGLAAVGYTEADLD
                     RLVEGTLPQHRVTKLSPRPALAEDLRTIFQNALRYW"
     gene            complement(2483..3388)
                     /locus_tag="VHR64_08895"
     CDS             complement(2483..3388)
                     /locus_tag="VHR64_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3302984.1"
                     /translation="MAELTQAIPTRTQVTAYTAARRKPLTYYAGRVGFWLLIIAILLY
                     TLFPFYWAIATSLKTSAELNQAPPLYWPKSLDFSHYDAVLHNGTFTAALKNSAIVAVL
                     SVLVALIIGSLAAYAIGRFNFRGKSAAMYLILSMTMFPAIAILGSLYDMIRWAGQFNT
                     KQALIFSYTTFTLPFMVWVLANFFRAMPAELEQAAMVDGASPMRAFYQILLPLAMPGM
                     ATTGLLAFIAAWNEYLFASTFTITPQQRTVPVAIANFSGNTSFEIPWGNIMAASVLVT
                     VPLIVLVMLLQRKIIAGLTAGAVKG"
     gene            complement(3392..4348)
                     /locus_tag="VHR64_08900"
     CDS             complement(3392..4348)
                     /locus_tag="VHR64_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3302985.1"
                     /translation="MASERLGITASATAPAGGRLRETAPVSEWAKARERLAWLFVAPA
                     ILVVAIVAIYPLIKTFQLSFTNARLGLGKKTEWVGLDNYRYLYHDQKFFDSLKHTIQF
                     TVASVGLETALGMIVALTIHSNFKGRGIVRTSMLVPWAIPTVVSAQMWKWMYIDVYGV
                     IPDFFGKKLHLMSPSTALLANPATSLWAIVAVDVWKTTPFMALLLLAGLQVIPGDIYE
                     SANIDGASKFRQFFDLTLPLLKPAILVALIFRTMDAFRVYDVVAVMKGQSPDTMTVAV
                     YTQNTLVGSQRLGRGSAASVVIFVIIGIFAFIYTRMIKVEEG"
     gene            complement(4452..5885)
                     /locus_tag="VHR64_08905"
     CDS             complement(4452..5885)
                     /locus_tag="VHR64_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3302986.1"
                     /translation="MTDSNRQIGDLFKEFRRGDINRREFLTRGTALGISAVFLGRMVT
                     DKAFAQDASPAAAAPGYSLKTPAGFDQSLAGQKITVVMGSDGPGTPFEQACVKLFTET
                     TGVEVNYIAGAQSTTDRLSNYLQILNAQASDIDAMMIDVIHPGILAIHALDLTDAMKA
                     NNVEYFKAIVDNNTVDGKLVGIPFYTDAGLLYYRKDLLEKYSISAPPATWTDLETSAK
                     TIMDGERGGGAGEFWGFVWQGNAYEGLTCDALEWQVSNGGGNIIEPDGTITVNNPQAI
                     ASFERAKAWIGTITPEGVTTYQEEDARNVWQGGNSAFMRNWPYAFSAGQSADSVIKDK
                     FAVTLLPKGDGEGARNAATLGGWQMMASKYSKSPDAATLFCRFMTSTEVERSSAIERA
                     LLPTIAAVYDDPDVLKANPYYADLKPVFQGGAVPRPSTVSSSSYNDVSTSYFTHVHNI
                     LTGSGDAASEMKACADELKTIMDELNA"
     gene            complement(6102..6905)
                     /locus_tag="VHR64_08910"
     CDS             complement(6102..6905)
                     /locus_tag="VHR64_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HEX3302987.1"
                     /translation="MTELTGVTKPIIGMVHLPALPGTPLFDANGGMKAIREWVKEDLD
                     ALQAGGIDAVMFCNENDRPYRLDSDFASVAAMADVVGTMRESLSVPFGVNVLWDPRAT
                     LAVAKATGAAFCREIFTGAFAGDFGLWVRCAGDSARYRREISAENVRMLYNINAEFAA
                     PIAPRPVADLARSVAFSSSPDAICVSGAITSQAANASDLELVANAVKGTGVPVLINTG
                     FKASNAETLLTFADGAIVGSSLKVDGVTWNKVDSTRVKELMAEVRRIRA"
     gene            complement(6945..7355)
                     /locus_tag="VHR64_08915"
     CDS             complement(6945..7355)
                     /locus_tag="VHR64_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX3302988.1"
                     /translation="MGIIRDLNEAQIEHLLRTAIVGRIACSHPDEPRPYLVPLAYGYD
                     GEAIYAHSGPGRKLINMRANPLVTIEVDAAEASDRWQSVIAEGRFEELNGTDRDRALR
                     LIYPEPAVIPELGSHTVVYRIVLTSKSGRYEEPD"
     gene            complement(7381..>7978)
                     /locus_tag="VHR64_08920"
     CDS             complement(7381..>7978)
                     /locus_tag="VHR64_08920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX3302989.1"
                     /translation="RDFIVTPAVLTPRPETEMLVEWAVKWLAHRTDARVVDVGTGSGA
                     IAIGVALGTPETVSITAIDISDSALAIARKNAERLCPGRITFRHGDLLTGFDEPVDLI
                     LANLPYLRPDQIDGNFDLVAEPRIALDGGRAGLALIKRLMKQLPGHLAEGGAVALEID
                     PSQAEPVGLGLSDTLPGANVRVHADLAGLDRFVSAVRL"
BASE COUNT         1483 a         2417 c         2595 g         1483 t
ORIGIN      
        1 gggcggctcg cccggcttcc cggggtcgat ggaccgggcg agccgcccgg cactacgttt
       61 tcttattcca agcaccgctc caagcgggac agggtaatcc cgcgctacgt ctagtgactc
      121 cacgatcccg ctgaactcca cggtagtgcg gctccccgtg gccggaacta cgtttcctgg
      181 atcggcacat cttcggagca ccgctgctct tgagtgagtc agatcttcag ggtgtggttc
      241 atgcgggcgt cgtcggccag gtttggaatg gccatcacgg tggtcatcag gggcaccatg
      301 accgggatgc gtggcctcag ctcgatctcg agcgagtgga ccggcatccc gcaaatcatc
      361 tgcacgctct gtccgccgcc ggtctgccac tcggtgtccg gcatcaccca gtcgattgac
      421 ttctcgggat gggccaccgc cgcgcagtag atggcgtaga ccccgtccgg gacgtgccgg
      481 attacgaagt tgcccggttc ctcgaggaca tcgccgagca agggccggcc ccggggcgga
      541 cctggtggga acagcccgac aaagtagtag gccggtcgac tcgcgtcccc gatgatccgg
      601 ccgctgacgg tgtgccgctc gtgcattcgc attaacggct cgcgctgaag ccggctgagg
      661 tcgtcggcca ggctgtcagc gaattcgacc gcgttccggt attcgctcgg tttcacgccg
      721 gcgagggtgg tgaagcgttg gctgaagctg ccgagactgc tgaacccgac cgcataacag
      781 gcgtccgtta ccgagctgcc ctcagcggcc agcagccgct tcgcttcctg gacccgcagc
      841 gcggtctgga tcgcggcggg cgtgtatccc gtgatctccc ggaaaacccg aatcacgtgg
      901 aacgggctga gcgccgcgat atcggccatg tccccgaccg acaacgtgcg ctctggttcc
      961 gtccgaacct gggtaatcac tcgctgtatt cgatcgcgat gatccgcctc agtaaccggg
     1021 cgcataggct cctcccttac cagtagcgca gggcgttctg gaagatcgtc ctgaggtctt
     1081 cggccaaagc cgggcgggga gagagtttgg tgacgcggtg ctgcggcagc gtgccttcga
     1141 ccagtcgatc gaggtccgct tcggtgtatc cgaccgcggc gagaccgttg ggcatgccgg
     1201 tcatgcgcat gatctcgatc aggcgattgg ccagaatgtc gccggcatcg tggacactgt
     1261 tgtcctgaat gtctgcgcca aggagctggg cagcgcataa atggcgatgc ggcgatgcct
     1321 gggccgtgaa tcggaagacg gccggcgcat tcaggatcac tgagatgcca tgcggcacga
     1381 tcgggtgatc gaccgcatac ccggccggtc gccaatcccg gaccataccg gcgaccgggt
     1441 aggacatgcc gtgtggcagg tggacgcccg cattgccaaa gccgatgccg gcgtaggtgg
     1501 cggcgagcat catctggccg cgcgcgtgga catcatcggg ccgctcgagc acgaccggta
     1561 ggcactgcgc gaccatctcc atcgccttcg acgcccaaat gtcgctgatc gggttcgcgc
     1621 cctggtaggc cggacgcagc ttcggcgcgt ccggagccgg ccgctgatcg aacgggaggg
     1681 cggtgtacga ctcgatagcg tggctgagga catcgagcgc cgatgaggcc gtcaccatcg
     1741 ccggcatcga gagcgtgttg agtggatcga cgatacccag cacagggcgc agagcacggt
     1801 gcgcgatacc ggtcttggcg tgcatctcga cgaggtcgaa gatcgcgacg ccggtcgtct
     1861 cgctcccggt gccggcggtc gtcgggatcg cgatcagcgg cttgaggggt cccgggacgg
     1921 gcgtggcttg gccgattggc gcgttaacgt aggtcagcag gtcggccggg tagctgtagt
     1981 agagattggc ggccttggcg gtgtcgatga ccgagccgcc gccaaatgcg acgaacccgt
     2041 ccggcttcgc ctcggtggca gcctcgatcg ccttgctgaa cgatgcgtcg gtcggctcga
     2101 cgcggacgtc gctgtactcg atgacatcga ggccggcagc tttcaggcta tcgcgcgcgg
     2161 ttgcatagcc atcggtcgcg gtcaggttct ggtcggagaa aagcatgacg cgcttgaggc
     2221 cgcgatcccg cgcatcatcg ccgacttccc gggtagcgcc cgggccgtat ttgatcgacg
     2281 aggtgtccat ggtgaagaca gattcgggcg tcgaagcagt catggcaatc ctccggaata
     2341 tcgaacatgt gtactataca gcagatgtcc gtacatgcaa gccctgatca tatctgacac
     2401 acggcgcggc atgaaagttc gccacaaaaa agccgtccgg ctaaaaatag agccggacgc
     2461 ctgagggatt gtgaatttgc ggttagccct tgacggcgcc cgccgtcagg ccggcgatga
     2521 tcttgcgctg gagcagcatc accagcacga tcagtggcac cgtgacgagt accgaggcgg
     2581 ccatgatatt gccccacggg atctcgaacg aggtgttgcc cgagaaattc gcgatcgcga
     2641 ccggcacggt gcgctgctgc ggtgtgatcg tgaaggtgct ggcgaacagg tattcgttcc
     2701 acgcggcaat gaatgccagc aggccagtcg tcgccatgcc cggcattgcg agcggcagca
     2761 ggatctggta gaaggcgcgc atcggactgg cgccgtcgac catagcggct tgctcgagct
     2821 cggccggcat cgcccggaag aagttggcga gcacccagac catgaacggc agcgtaaatg
     2881 tcgtgtacga gaagatcagg gcctgcttgg tgttgaactg gccggcccac cggatcatgt
     2941 cgtagagcga gccgaggatt gcgatcgccg ggaacatcgt catcgacagg atcaggtaca
     3001 tcgcggccga cttgccgcgg aagttaaagc gaccgatggc gtatgcggca agggaaccga
     3061 tgatcagcgc gaccagcacg ctcagaaccg cgacgattgc cgagttcttg agcgcggcgg
     3121 tgaacgtgcc gttgtgcagc accgcatcgt agtgcgagaa gtcgagcgac ttgggccagt
     3181 agagaggagg cgcctgattc aactcagccg aggtcttgag cgaggtcgca atagcccaat
     3241 agaacgggaa aagcgtgtag agaaggatgg cgatgatgag gagccagaac cccacccgcc
     3301 ccgcgtaata ggtcaggggt ttgcgacgcg ctgcggtgta ggcggtgacc tgggttcgtg
     3361 ttggaatggc ttgcgtcagc tcggccatgg gttaaccctc ctccaccttg atcatgcgtg
     3421 tgtagatgaa agcgaagatg ccgatgatca cgaagatgac cacggatgcg gcggaacctc
     3481 tcccgaggcg ttgactgccg acgagtgtgt tttgcgtata gacggccacc gtcatggtgt
     3541 cgggcgattg ccctttcatc acggccacca cgtcgtacac acggaaggcg tccatggtgc
     3601 ggaagatcaa cgctaccagg atcgcaggtt tcaggagtgg cagtgtcaga tcgaaaaatt
     3661 ggcggaattt ggacgcgcca tcgatattgg ccgattcgta aatgtcccct ggtatgacct
     3721 gaagacccgc tagaagcagc agagccatga acggagtcgt cttccagaca tcgacggcga
     3781 caattgccca gagcgatgtc gccgggtttg caagcagcgc cgtgcttggc gacatgagat
     3841 gcaacttctt gccgaagaag tcggggatga cgccgtagac gtcgatgtac atccacttcc
     3901 acatctgcgc agagacgacg gtgggaattg cccacggcac cagcatcgat gtgcggacaa
     3961 tgcctcggcc cttgaaattc gagtggatcg tcagcgccac gatcatgcca agtgcggtct
     4021 caagacccac cgacgcgacc gtgaattgga tcgtatgctt gagcgaatcg aagaactttt
     4081 ggtcgtggta aagatatctg taattatcga gacccaccca ctcggtcttc ttgccgagtc
     4141 caagccgggc attagtgaac gaaagctgaa atgtcttgat caggggataa atcgcgacga
     4201 ttgcgacgac gagaatcgcg ggagcgacga agagccatgc caaccgttcc cgcgccttgg
     4261 cccattcact gaccggcgcc gtctctctca ggcgcccacc ggccggggca gtggccgacg
     4321 cggtaatgcc taaacgctcg cttgccatag cctcaccctc cctcgttgga ggtacaggac
     4381 cgggcggagg caccgcgccc ccgcccggtc tgctaacacc ctatcacccc aaggcaatga
     4441 gcccggaatg cctaggcgtt cagctcgtcc atgatggtct tgagctcgtc cgcacaggcc
     4501 ttcatctcgg atgcggcgtc gccggatccg gtcaggatgt tgtgaacgtg cgtgaagtac
     4561 gaggtcgaga catcgttgta ggagctgctg gagaccgtcg acggccgcgg gaccgcgcca
     4621 ccctggaaga ccggcttcag gtcggcgtag tacgggttgg ccttgagcac gtccggatcg
     4681 tcgtagaccg ccgcaattgt cggcaggagt gctcgctcaa tcgcagaaga ccgctcgact
     4741 tccgttgagg tcatgaaccg gcagaacagc gtggcagcgt ccgggctctt ggaatacttg
     4801 gacgccatca tctgccaccc accaagggtc gcggcgtttc gagcgccttc gccgtcgccc
     4861 ttcggcagca gcgtaacagc gaatttgtcc ttgatgaccg agtccgcact ctggcctgcc
     4921 gaaaaagcgt acggccagtt gcgcatgaag gcggagttgc cgccctgcca gacgttccgc
     4981 gcgtcctctt cctgataggt tgtcacacct tcgggcgtga tcgtgccgat ccaggccttg
     5041 gcgcgctcga acgaggcgat cgcctgcggg ttgttgacgg tgatcgtgcc gtccggctcg
     5101 atgatgtttc caccaccgtt cgagacctgc cactcgaggg cgtcgcaggt caggccctcg
     5161 taggcattgc cttgccagac aaagccccag aactcgccag cgccgccgcc acgctcgcca
     5221 tccatgatgg tcttggcgga cgtctcgaga tcagtccagg tcgccggcgg tgccgagatg
     5281 ctgtacttct cgagcaagtc cttgcggtag tagaggagac cggcgtcggt atagaacgga
     5341 atgccgacca gcttgccatc gaccgtgttg ttgtcgacga tcgccttgaa gtactcgaca
     5401 ttattggcct tcatcgcatc ggtcaggtcg agtgcgtgga tcgccaaaat acccgggtgg
     5461 atgacgtcga tcatcatcgc gtcgatgtca cttgcctggg cattcaggat ctggaggtaa
     5521 ttcgagaggc ggtcggtcgt tgactgcgca ccggcaatgt agttcacctc gacgccggta
     5581 gtttccgtga agagcttgac acacgcctgc tcaaacggag tgcctgggcc gtcggagccc
     5641 atcacgaccg tgatcttttg gccggccagg ctctggtcga atccggctgg ggtcttgagc
     5701 gagtagccgg gagcggctgc cgccggggaa gcgtcctgcg cgaaggcctt gtcggtcacc
     5761 atgcggccga ggaagacggc gctgatgcca agggcagttc cgcgagtcag gaattcacgg
     5821 cggttgatat caccgcggcg gaattccttg aagaggtcgc cgatctgacg attactgtcg
     5881 gtcactgctc aactccgttc actgagtcgt gcgcggcgca catcgccgcc ctttggcacc
     5941 cgctcaccct cccggtgttt ggaagagcgc tggcgagaca gttgccggag ttcttgtagt
     6001 accacgcaca ttcgagcaag gtcaaggcaa tgcgcgacac tcgcccctaa atcgtattgc
     6061 tacgttttgc aaaggttatg cgctgagttc attagccatg ctcaagcgcg gatgcggcgg
     6121 acctccgcca tcaactcctt gacccgcgtc gagtcaacct tgttccaggt aacgccgtcg
     6181 actttgaggc tggagccaac gatcgcgcca tcggcaaagg tcagcaatgt ctcggcgttg
     6241 ctggccttga acccggtgtt gatcaggacc ggcacgccgg tgcctttgac cgcattggcg
     6301 accagctcga ggtcggacgc gttggcggcc tggctggtaa ttgcgccgga aacgcagatc
     6361 gcgtccgggc tggaggagaa ggcgaccgag cgggccagat cggccaccgg gcggggcgcg
     6421 atcggcgcgg cgaactcggc attgatgttg tagagcatgc gcacattctc ggcgctgatc
     6481 tcgcggcgat accgggcgga atccccggcg cagcgaaccc agagcccgaa gtcgccggcg
     6541 aatgcgccgg tgaagatctc gcgacagaag gcggcaccgg tcgccttggc cacggccagg
     6601 gtcgcgcgcg gatcccacag gacgttgacg ccgaacggta ccgagagact ctcgcgcatc
     6661 gtaccgacca cgtcggccat ggccgcgacc gacgcgaagt cggagtcgag tcgatatggc
     6721 cggtcgttct cgttgcagaa catgacggcg tcgatgccgc cggcctgcag cgcgtcgagg
     6781 tcttccttga cccactcgcg gatggccttc atgccaccgt tggcgtcgaa gagtggcgtc
     6841 cccggcaagg ccggcaggtg aaccatgccg atgatcggct tggttacgcc cgtcagttca
     6901 gtcaaccagt tcccgtacac gattcctccg atgcttcccg gccctcagtc cggctcctcg
     6961 tatcgacccg atttcgatgt cagcacgatc cggtagacga cagtgtgcga ccccagttcc
     7021 ggaatgactg ccggctcggg gtagatcagc cgaagcgcgc ggtctcgatc agtcccgttc
     7081 aactcctcga atcgaccctc ggcgatcacg ctctgccagc gatccgaggc ctcggccgca
     7141 tctacctcga tcgtgaccag agggttggcc cgcatgttga tcagcttgcg gccgggacca
     7201 ctgtgggcat aaatcgcctc gccgtcgtac ccgtacgcaa gcggaaccag gtacggccgt
     7261 ggctcatcgg ggtgactgca cgcgatgcgg ccgacgatcg ccgtccgcaa caggtgctcg
     7321 atttgcgcct cgttcaggtc ccggatgatg cccacggtct cgtgcctccc ccgcgcgtca
     7381 tcatagccgg acggcggaga cgaatcggtc cagccctgcg aggtcggcgt gcacgcgaac
     7441 gtttgcgccg ggcagtgtgt ccgaaagacc caggccgacg ggctccgcct gggatggatc
     7501 aatctcgagc gcgaccgctc cgccctccgc gaggtggccg ggcaattgct tcatgaggcg
     7561 cttgatcagc gcaagtcccg ctcggccgcc atcgagtgcg atccggggct cagccacgag
     7621 gtcgaagttg ccgtcgatct ggtcgggccg aagatagggg agattggcaa ggatgagatc
     7681 gaccggctca tcgaatccgg tcagaaggtc gccgtgacgg aacgtgatcc ggcccggaca
     7741 caatcgctcg gcgtttttcc gggcaatggc aagagccgag tcggagatgt cgattgcggt
     7801 gattgagacc gtctcaggcg tgccgagggc gactccgatc gcgatcgccc cgctgccggt
     7861 tccgacatcg accacgcgcg cgtcggtccg atgggcgagc cacttgacgg cccattcgac
     7921 gagcatctcg gtttccggcc gcggcgtcag gactgccggg gtgacgatga agtcgcga
//
LOCUS       DATAOZ010000348         3971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2248835, whole genome shotgun sequence.
ACCESSION   DATAOZ010000348 DATAOZ010000000
VERSION     DATAOZ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2248835"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1317
                     /locus_tag="VHR64_08925"
     CDS             259..1317
                     /locus_tag="VHR64_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX3302990.1"
                     /translation="MSQQDDGEQVLDVLGKDPRLIPQEATNYESPLALLDTFLTPIER
                     FFIRSNGSVSVDVDREAWRLNVTGLVERELSLSLADLTAMPIRTITAFLECSGNSRSR
                     FPADPAKVEGTNWGNGAVGNAEWTGVPLRHVLEQAGVGEGVVEVVSQGGDFARMRRGL
                     PIETALSPDVLLAWKMNGQDLPAAHGGPVRLLVPGWGAIASTKWIIGLELIDSAFDGY
                     YNADAYVLYDQTGTPTGRVTQMPVKSIITSPAPGSNLSAGPQTIAGFAWSGHGGIARV
                     EVSTDGGATWTEARIVQAAGPLSWVRFEHRWRAEPGESRLQSRATDAQGNIQPQHAAW
                     NAKGYQMNAIDEVTVTVR"
     gene            complement(1333..2730)
                     /locus_tag="VHR64_08930"
     CDS             complement(1333..2730)
                     /locus_tag="VHR64_08930"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX3302991.1"
                     /translation="MWSADQLRSGLLTDLYHVDSAYVSWRSGRNGLTTFDLYSRSNPF
                     GSGYMLAAGLELAVTFAREFRYGEEEIAYLKRVKPYEDSFYDELRRLRFTGEILGMPE
                     GEIAFAPEPLLRVTAPFREALLLEAGLLHVISLSTLIATKAARIVQSARGKGVAEFGF
                     RRAQEPYIAARSGYIAGCASTSFLAAACAFGIPTSGTIPHALIQSFPTEEEAFRTVAE
                     ALPRYTLLLDTYDVRQAIQTAVEVGREARDKYGHRLAGVRLDSGDILADSLHCRQVLD
                     EAGMADVQIVASGDMDEFRIAELEGAGAPIDAYGVGTALAVGAGSIEHGISGGALGGV
                     YKLVWYDADADQTADSTEAPIKVAGPKSTWPGKKQVSRIGVFREDVIHLEDEAPLPEG
                     RSLLEPVLRGGELVPGVLPSLNEILDRAARNLAALPPEYRAIEHPAGYPVRNSDRLRD
                     MRKRAMEQHGNGVES"
     gene            complement(2752..3468)
                     /locus_tag="VHR64_08935"
     CDS             complement(2752..3468)
                     /locus_tag="VHR64_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX3302992.1"
                     /translation="MTASLSLESVGTAIPAWVAEWFAALQPLPLAEIVVDPTSVGLFS
                     ADMIVGFCDSGNLASPRIDELTGPVVDLFTRAHGLGVREFVLMQDTHDPNTPEFGAWP
                     VHCVAGTDESETIPELKELPFSDPFTVIPKNALGPGYGTNFDDWLDAHPRITTAIVVG
                     DCTDLCTYNLAMHLRMRANARNIQGVEVIVPANAVDTYDLPPEQARAISAFAHPGDFF
                     HQVFLYHMALNGIRVVSELS"
     gene            3729..>3971
                     /locus_tag="VHR64_08940"
     CDS             3729..>3971
                     /locus_tag="VHR64_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEX3302993.1"
                     /translation="MGTYALLLVAIAIEVVATTALKASDGMSKLIPSVIVIIGYVVSF
                     YMVAVILKRMDLGVVYATWSALGTAAIAIIGVVIYDD"
BASE COUNT          681 a         1288 c         1340 g          662 t
ORIGIN      
        1 gtgttctcga agacgacatc ctcggtcatc aacgccgaaa tcgcggcgac gtcgtggcga
       61 tcaaacgcct cgttgaagcg aatgatgacg gtccgggtat ccgtagcgac atctgttgtg
      121 ctcacgtgct cctcctcgcg cagggtggcc gacaacgtaa cggtgtgcga tgcaaagatt
      181 gtatctgtcc tcgcaagtag gcccattggc ggtgatagag tgacgacttg gagatgaaac
      241 gccggagaag gagcgagcgt gagccagcag gatgacggtg agcaggttct cgatgtcttg
      301 ggcaaggacc cgcgcctgat cccgcaggag gcgaccaatt acgagtcacc cctggccctg
      361 ctcgacacgt tcctgacacc gatcgaacgc ttcttcatcc gctccaacgg ctcggtttcg
      421 gtcgacgtcg accgggaggc atggcggctc aacgtgactg gactggtcga gcgggagctg
      481 tcgctgtcgc tggccgacct cacggcgatg ccgatccgga cgatcaccgc gttcctcgag
      541 tgctccggca atagccgcag ccgcttcccg gccgacccgg cgaaggtcga gggcaccaac
      601 tggggcaacg gcgcggtcgg caacgcggag tggaccggcg tcccgctccg ccacgtcctc
      661 gaacaggcgg gcgtgggaga gggtgtggtc gaggtcgtct cgcagggcgg cgatttcgcc
      721 cggatgcgtc gcggcctgcc gatcgagacc gcattgagcc cggatgtcct gctcgcctgg
      781 aagatgaacg ggcaggatct gccggcggcg cacggcgggc cagtccggtt actcgtcccg
      841 ggctggggtg cgatcgcctc gaccaagtgg atcattgggc tggagctgat cgactccgcc
      901 ttcgatggct actacaacgc cgatgcctac gtgctctacg accagaccgg cacgccgacc
      961 ggccgggtga cgcagatgcc ggtcaagtcg atcatcacct cgcccgcgcc tggatcgaat
     1021 ctatccgccg ggccgcagac aatcgccggc ttcgcgtggt ccggccatgg cggaatcgcg
     1081 cgggtcgagg tcagcaccga cggcggcgca acctggacgg aagcgcggat cgtgcaggcg
     1141 gccggaccgc tctcctgggt ccgcttcgag catcggtggc gggccgagcc cggcgaatcc
     1201 cgcctccaat cccgcgccac cgacgcccaa ggcaacatcc agccgcagca cgccgcctgg
     1261 aacgccaagg gctaccagat gaacgcaatc gacgaagtta ccgtaaccgt tcggtaagaa
     1321 agcgctttcc cctcaactct ccaccccgtt gccgtgctgc tccatggcgc gcttgcgcat
     1381 gtcgcggagg cggtcggagt tgcggaccgg gtacccggcc ggatgctcga tcgcccggta
     1441 ctcgggcgga agggcggcga ggttgcgggc ggcgcggtcc aggatctcgt tgagggaggg
     1501 cagtacgccg gggaccagct cgccgccacg gaggaccggt tccagcagcg agcggccctc
     1561 gggcagcggg gcctcgtcct cgagatggat cacgtcctcc cggaagacgc cgatccgcga
     1621 gacctgcttc ttgcccggcc aggtgctctt cggcccggcc accttgatcg gcgcctcggt
     1681 cgaatccgcg gtctggtccg catcggcgtc gtaccagacc agcttgtaga caccgcccag
     1741 cgcaccgccc gaaatcccgt gctcgatcga tccggccccg accgccaacg cggtcccgac
     1801 cccgtaggca tcgatcggcg caccggcacc ctccagctcg gcgatccgga actcgtccat
     1861 atccccgctg gccacgatct ggacgtcggc catcccggcc tcatcgagca cctgccggca
     1921 gtggaggctg tcagccagga tgtcgccgct atcgaggcgg acgccggcca gccggtggcc
     1981 gtacttgtcc cgcgcctcgc ggccgacctc gaccgcggtc tggatcgcct ggcggacatc
     2041 atacgtgtcg agcagcaggg tgtagcgggg caacgcctcg gccacggtcc gaaacgcttc
     2101 ctcctcggtc ggaaagctct ggatcagcgc atgcgggatc gtgccgctgg ttgggatccc
     2161 gaaggcacag gcggcggcca ggaacgaggt gctcgcgcag ccggcgatgt agccggagcg
     2221 ggccgcgatg tacggctcct gcgcgcgccg gaaaccgaac tcggccacgc ccttgccccg
     2281 tgccgactgc acgatccggg ccgccttggt cgcgatcaag gtcgagagac tgattacgtg
     2341 gagcaggccg gcctcgagca gcagcgcttc gcggaacgga gcggtcacgc gcagcagcgg
     2401 ctcgggtgca aaggcaatct caccctccgg catgcccagg atctcgccgg tgaagcggag
     2461 ccggcgaagc tcgtcgtaga aggaatcctc gtacggcttg acccgcttca ggtaggcgat
     2521 ctcctcctcg ccgtagcgga actcccgtgc gaaggtgacg gccagctcca gcccggccgc
     2581 gagcatgtag ccggagccga aggggttgga gcgcgagtag aggtcgaacg tggtcagccc
     2641 gttgcgcccg gatcgccagc tcacgtaggc ggaatcgacg tggtagaggt cggtcagcaa
     2701 tccgctccgt agctggtcag cgctccacat tgggtggcgt actccgtttc atcaggacag
     2761 ttcggacacg acccggatac cgttcagggc catgtggtag aggaaaacct ggtggaagaa
     2821 atcacccggg tgcgcaaagg cgctgatcgc ccgggcctgc tccggcggca ggtcgtaggt
     2881 atcaaccgcg ttggccggca cgatcacctc gactccctgg atattgcgcg cgttggcccg
     2941 cattcgcagg tgcatcgcca ggttgtaggt gcagagatcg gtgcaatccc cgacgacgat
     3001 cgcggtcgtg atccgcgggt gcgcgtcgag ccaatcgtcg aagttcgtgc cgtagccggg
     3061 gccaagcgca ttcttcggga taaccgtaaa cgggtcgctg aacggcaatt ccttcagctc
     3121 cgggatcgtc tcgctctcgt cggtgccggc gacgcagtgg accggccaag cgccgaactc
     3181 gggcgtgttc gggtcgtgcg tatcttgcat caggacgaac tcgcgcacgc cgagaccgtg
     3241 ggcgcgggtg aagagatcga cgaccgggcc ggtcagctcg tcgattcggg gcgaggcgag
     3301 gttcccggag tcgcagaagc cgacgatcat gtcggccgag aagagtccga ccgacgtcgg
     3361 gtcaacgacg atctcggcaa gcggcagcgg ctgaagcgcg gcgaaccact cggcaaccca
     3421 ggccgggatc gcggttccga ccgattccag cgagagcgac gcagtcatcc agccacctcc
     3481 tcgtggtcga caccatacac gcccaagtgt atcgatctct gagctgccct gattctctcc
     3541 gtagtggcgg ggctctgtac ccgccttgac ctcacgacgg acccggtatt tgcaccgacc
     3601 atcttccccg gttggaatcg atgagcgcgc ggtgcaaatg aacgccttgg tcggcggcac
     3661 ctctgggcgg gtacggagcc ccgccactac gattcggtgt gcattgttgc gacgccgggg
     3721 agagactgat ggggacgtat gcgctgttgc tggtggcgat cgcgatcgag gtggtagcga
     3781 cgaccgcgct caaggcctcg gatgggatgt cgaagctgat cccgagcgta atcgtcatca
     3841 tcggctacgt cgtttccttt tatatggtgg cggtgatcct gaagcgcatg gacctcggtg
     3901 tggtctacgc cacctggtcg gcgctcggca cagcggcgat cgcgatcatc ggggtcgtga
     3961 tctacgacga t
//
LOCUS       DATAOZ010000349         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_405398, whole genome shotgun sequence.
ACCESSION   DATAOZ010000349 DATAOZ010000000
VERSION     DATAOZ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405398"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VHR64_08945"
     CDS             <1..239
                     /locus_tag="VHR64_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302994.1"
                     /translation="GLGAAVVAVLRLPLSAVVLATLFTVHTGLGSGPLIIVGVVVAYL
                     TVQALRGRAESSGGSPADGTGANGRSEPAAMPSG"
     gene            complement(310..975)
                     /locus_tag="VHR64_08950"
     CDS             complement(310..975)
                     /locus_tag="VHR64_08950"
                     /inference="COORDINATES: protein motif:HMM:NF022589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HEX3302995.1"
                     /translation="MAEVWVELGLVALAAMVSPTTLAFSIFALMLGDRPLRTGFWFYV
                     GALGITLIIGVAGAFIIGDVAASHSSNPKTWVAVVDIMAGVLVLGYVVHALRRPRDPE
                     RMASMMERMSKLASSPVIAIVGAGAILANPGGFIPLALKDISQLNPDTGQYIVAWLFF
                     SLISLLPMAIALLMLILAREHAMRILGAARAWLERHGRTVAAVILVLLAAVLLRNGIA
                     GLA"
     gene            1252..1692
                     /locus_tag="VHR64_08955"
     CDS             1252..1692
                     /locus_tag="VHR64_08955"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX3302996.1"
                     /translation="MKAGDLIGTWRLRSWRNVANDGSAVDPLGESPIGYIFYNHDGYM
                     SVEIMAAHRAPYHDPDAFGGTPEERSEAISTYLSYSGPYEVLPDRETVIHHIEVCSYP
                     NWIGNAQVRFAKLHDDLLELSTKPMTFQGVERRAELVWERVGDH"
BASE COUNT          387 a          668 c          666 g          350 t
ORIGIN      
        1 tgggcctggg cgccgcggtc gtcgccgtgc tgagattgcc cctgtccgcg gtcgtgctcg
       61 ccacgctctt cacggtgcat acggggttgg gctcgggacc cctgatcatc gtgggcgtgg
      121 tggtggcata tctgaccgtg caggcactcc gcggccgtgc cgagtcctcc gggggatctc
      181 cggcggatgg aaccggtgcc aatgggaggt ctgagccggc ggcgatgcca agcggctgac
      241 cgcgaccgct cctccctaag gctctgcccc tggtttttcg agcctctgtc ctacctgctt
      301 ttgccgccat caggcgagac cggcgatgcc attcctcagc agcaccgctg ccaacagaac
      361 caggatcacg gccgcgacgg tccgcccgtg tcgctcgagc caggctctcg cggcgccgag
      421 gatccgcatc gcatgctcac gagcgagtat caacatcagc agcgcgattg ccatggggag
      481 cagtgagatc agggaaaaga acagccaggc gactatgtac tgcccggtat ctggattgag
      541 ctgagagatg tccttcagcg ccagcgggat gaaaccgccg ggattcgcga ggatggcgcc
      601 ggcgcccacg atcgcgatca ccggagacga ggccaacttg ctcattcgtt ccatcattga
      661 tgccattcgc tccgggtcgc gcggccgcct caatgcatgc accacgtagc cgagcacgag
      721 gacaccggcc atgatgtcga ccaccgccac ccacgtcttc gggttggagg aatgtgacgc
      781 cgccacgtca ccgatgatga aagccccggc gaccccgatt atgagcgtga tgccgagcgc
      841 cccaacatag aaccagaacc ccgtgcggag cggccgatcg ccgagcatga gggcgaagat
      901 cgaaaacgca agcgtcgtgg gcgacaccat cgccgccagg gcgaccaaac cgagctccac
      961 ccaaacctcg gccatcgctg caccctagtt cacgtcttca tgttcgattc gtgtgccgat
     1021 ttcaccgctg gcgccgacgc caacaagtcg cccctgccgc ggtcgtcgcc gtactgagat
     1081 tgcctctgtc cgcggtgtcc gaccgcgccg cgcaaaaacg aagtgacgcc tgacgtgcac
     1141 ccaggacacc ttgcccgcaa cgccgtcgaa ctggcgggag ccgccgagct tctcgggtta
     1201 tgctgaaggc gcgctggagt caggactgtg ggtccttcga ggaggttgaa gttgaaggct
     1261 ggcgatctga tcgggacctg gcgactccgg tcgtggagga acgtcgcgaa cgacggatcg
     1321 gcggtcgatc cgctgggcga gagcccgatc ggctacatct tctacaacca cgacggctac
     1381 atgtcggtgg agatcatggc tgcgcaccga gccccgtatc acgacccgga cgccttcggc
     1441 ggcactccgg aggagcgatc ggaggcgatc agcacctacc tgtcctactc ggggccctat
     1501 gaggtgctgc cggaccggga gacggtcatt caccacatcg aggtctgctc atatccgaac
     1561 tggatcggaa acgcccaggt ccgttttgca aagctgcacg acgatctgct cgagctcagc
     1621 accaagccga tgacgtttca aggcgtggag cgtcgggcgg agttggtctg ggagcgagtc
     1681 ggtgaccact gacgcgggcg cttcgctgat ggcctctggg cgaagcatcg acttgcaagc
     1741 ccttcgaagg cctcaggttc ttccacgagc cgccgttcag cgactaaccg acaccctccc
     1801 ctcttcgacc aggaagcaat cggccacagc cgtaggtggg agcgaagagg cccgtgccgc
     1861 agtcgctact cgtagggatc ggcgctggag cactcagcgg ctagtctcac ccgatgagcg
     1921 agccagctcc cggattcaag caaggccagg cggtctgggt cgaggacggc gacgggaaaa
     1981 tgcatcccgg gaaattcgtc ggcgagaccg agagtgtgag ttggctgggc ggcgttccga
     2041 gcgctcgcgt cgtggacccc gagactaacc a
//
LOCUS       DATAOZ010000350         4186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1194900, whole genome shotgun sequence.
ACCESSION   DATAOZ010000350 DATAOZ010000000
VERSION     DATAOZ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194900"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VHR64_08960"
     CDS             complement(<1..441)
                     /locus_tag="VHR64_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3302997.1"
                     /translation="MDTAHRLWRQAIQDRLPALVVAAIVVGIAIGMDGDGRRFVNGIG
                     GILWLIGAFLIFTRSLASGVSWRQIGLVIAVILVLSWLIRPTDPVWAVIGFGWGGVVV
                     GFSGRDLGSQLGAMLGALWLPAHLLIAVVRVGIREVRDQPAALRT"
     gene            568..1506
                     /locus_tag="VHR64_08965"
     CDS             568..1506
                     /locus_tag="VHR64_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX3302998.1"
                     /translation="MGPHSTRPFRFAIQSQRADSAQEWRAKAKKIEALGYDVLLMADH
                     FGPQFGIAPALAVAAEATTTLRLGQLVLQNDLRHPALLAKDVATLDLLSDGRFELGIG
                     AGGSYPPDFDWTGIPFDPAPVRVDRLRESIAVLKGLFSDGPFTYEGRHFRICEYDGMP
                     KPTQKPWPPILIGAGGRRMMELAAREADIIGLLPAMGPTGGDFGLDEMTVPGLAQKVE
                     YIRSIAPDRFDAIEFNSLTQVFEVTDSRQAVIDRLSSEWEQDPADWKDSPFLLIGSEE
                     SIADDIRSYRETLGFTYFVLRDHMMDSFAPILARLK"
     gene            1565..1640
                     /locus_tag="VHR64_08970"
     tRNA            1565..1640
                     /locus_tag="VHR64_08970"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1598..1600,aa:Arg,seq:tcg)
     gene            1689..2852
                     /locus_tag="VHR64_08975"
     CDS             1689..2852
                     /locus_tag="VHR64_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX3302999.1"
                     /translation="MRAQILSIGEELLGGYITDTNSTFLEQQLALLNIPVTLVTQVGD
                     NRDRIAEVITRAMRDADIVVCTGGVGPTEDDLTREAIAYVLGEEPVIDHGLLEVIRNF
                     FANRGLEMPERNQKQAWVIPSCQPLPNPVGTAPGWYVRTGKKIIVAMPGVPREMYRMW
                     SEQVIPRISTLRFDQIVRSTTLKTIGIGESMVEQTLDTLVKRADPVIATYAKDDGVHV
                     RVTAIAETEQEASAKRDTCVAEIENLIGTYIYGRDDDSLAGALLGLLQSLDLTISICD
                     AGGGGRFASLLAENPGADPLLITSTMQPIGSAGLAQQFAEFATESEATLGLGISVSYE
                     PTGSGTWAALVEVAVAGARTGAKSFPIRTSYEEVQRRSALFAAEVLRSALISD"
     gene            complement(2842..3294)
                     /gene="ybeY"
                     /locus_tag="VHR64_08980"
     CDS             complement(2842..3294)
                     /gene="ybeY"
                     /locus_tag="VHR64_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX3303000.1"
                     /translation="MSAHGITADVTVIRKTPVPDAVTEERISDLVTFCLSEEHASGPW
                     QVAIAFVDEREIGLLHDQFMGDPSPTDIITFPYDDPDFSGGDIAICVTVASDNAHDQG
                     NSLSKELGFLVLHGMLHLLDYDDTADADRAAMLARQQVILDKWIALNR"
     gene            complement(3294..3752)
                     /locus_tag="VHR64_08985"
     CDS             complement(3294..3752)
                     /locus_tag="VHR64_08985"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX3303001.1"
                     /translation="MTDSVKARMEADLKDAMKAGDVTARETLRFTLAALKNAEIDKRS
                     ALSPSEEMDVLKTQAKRRGDSIDQYRAAGRADLVDRESAQLEVLKRYMPTEMSDDELA
                     ALVRDAIARVGASSPKEMGKVMPVALKAAGDRASGKRVSDAVRQALAGGA"
     gene            complement(3756..>4186)
                     /locus_tag="VHR64_08990"
     CDS             complement(3756..>4186)
                     /locus_tag="VHR64_08990"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX3303002.1"
                     /translation="LQGVRAWWLVGLPVSPAAGEFGRSWTGQAMDDCIFCKIVRGEIP
                     SSRVAESDRAIAFEDIAPKAPVHTLVIPKEHIAALHEATGADREMLADCLLLCNEVAR
                     IKDIDLSGFRVVTSDGPDGGQEVAHLHFHVLGGRKLQFGV"
BASE COUNT          791 a         1376 c         1286 g          733 t
ORIGIN      
        1 ggtccgcagc gcagccggtt gatcccgcac ctcacggatc ccgacccgga ccaccgcgat
       61 caggagatgg gcaggcagcc aaagcgcacc gagcatcgcg ccgagttgcg aaccaagatc
      121 ccggccagag aatccgacga ccacgccgcc ccacccgaat ccgattaccg cccaaaccgg
      181 atcggtgggc cgaatcagcc acgagagcac gaggatcacg gcgatgacca ggccgatctg
      241 ccgccagctt acgcccgatg ccagcgagcg cgtgaagatc agaaaggcgc cgatcagcca
      301 gaggatgccg ccgattccgt tcacgaatcg gcgtccatcg ccgtccatgc caatggcgat
      361 cccgacaaca atggccgcca cgacgagtgc cgggagtcga tcctgaattg cctgtcgcca
      421 gagccgatgc gccgtatcca tgaccgcaat ggtgcgcagc agctagtcga agcgtcaatc
      481 gagcgcggca gccatgcaac gactgggcaa gccgcatgcg ggtatgatca gcagccgcgg
      541 attgctatcg gacagcaagg agcagctttg ggaccgcatt cgacacggcc atttcggttt
      601 gcgattcaga gccagcgggc ggactccgcg caggaatggc gcgcgaaggc gaagaagatc
      661 gaggcgctcg ggtacgacgt gctgctaatg gctgatcact tcggcccgca attcggcatc
      721 gcgccggcgc ttgcggtcgc ggccgaggcg acgacaaccc ttcgactagg ccagctcgtc
      781 ctgcaaaacg atcttcgcca cccagcgctg ctcgccaagg acgtcgccac gctcgatctc
      841 ctttcggacg gtcggttcga gctcggaatc ggcgcgggtg gatcgtatcc gcctgacttc
      901 gactggaccg gcattccctt cgacccggcg ccggttcggg tcgatcgtct tcgcgagagc
      961 atcgcggtgc tcaaaggctt gttctcggat ggcccattca catacgaagg ccggcacttc
     1021 cggatctgcg agtacgacgg catgcccaag ccgacccaga agccgtggcc gccgatcctg
     1081 attggtgcgg gtggacgacg catgatggag ctcgccgccc gggaggccga tatcatcggc
     1141 ttgctgcccg cgatgggccc gaccggcggg gacttcgggc tcgacgagat gaccgtgccg
     1201 ggtctggcgc agaaggtcga gtacatccgc tctatcgccc cggaccgttt cgatgcgatc
     1261 gagttcaaca gcctgacgca ggttttcgag gtcaccgaca gcaggcaggc ggttatcgat
     1321 cgcctgagct cggaatggga gcaggacccg gctgactgga aagattcgcc ctttctgctg
     1381 atcggcagcg aggagagcat cgccgatgac atccgcagtt accgggaaac gctcggattc
     1441 acctacttcg tgctccgcga tcacatgatg gacagcttcg cgccgatcct ggcccgactc
     1501 aagtaacact ggcggaagat ccagcgattt cagagagcag cgtgctagaa ttccgccgac
     1561 gcctgcccct gtagctcaac ggatagagcg tctgacttcg aatcagaagg ctgggggttc
     1621 gagtccctcc gggggtacca gacccgctcc gatttccggg cgggtctttt cgttggaggc
     1681 agcaccggat gcgcgcgcaa atcctctcga tcggcgaaga gctcctcggc ggctacatca
     1741 ccgacaccaa cagcaccttc ctcgagcagc agctcgccct cctcaatatc ccggtcacgc
     1801 tcgtcacgca ggtcggcgac aaccgggacc ggatcgctga ggtcatcacc cgggccatga
     1861 gggacgccga tatcgtcgtt tgcacgggcg gggtcggacc gaccgaagac gacctgaccc
     1921 gggaagcgat cgcctacgtc ctcggcgagg aacccgtcat cgaccacggg ttgttggagg
     1981 taatccgcaa cttcttcgcc aatcgcggcc tcgagatgcc ggagcgcaac cagaagcagg
     2041 cctgggtaat cccgtcctgc caaccactgc cgaatccggt cgggaccgcg ccaggctggt
     2101 acgtccggac cggcaagaag atcatcgtcg cgatgcctgg cgtgccgcgc gagatgtacc
     2161 gaatgtggtc ggagcaggtc atcccccgca tctcgacgct tcgtttcgac caaatcgtgc
     2221 gctcgacgac cctgaagacg atcggcattg gcgagtcaat ggtcgagcag acactggaca
     2281 cgctggtcaa gcgcgcggac cctgtcatcg cgacgtacgc caaggacgac ggggtgcacg
     2341 tgcgcgtgac tgcaatcgcc gagaccgaac aggaggcatc cgccaaacga gatacgtgcg
     2401 tggccgagat cgagaatctg atcggcacgt atatctacgg tcgcgacgac gattcgttgg
     2461 ctggcgcgct gctggggctg ctgcaatcgc tcgacctcac gatttcgatt tgtgacgccg
     2521 gcggcggtgg gagattcgcc tcgcttctcg ccgagaaccc gggcgcagat ccgctgttga
     2581 tcacgagcac gatgcaaccg atcggttcgg ccggccttgc ccagcagttc gcagagtttg
     2641 cgaccgagag cgaagccacg cttgggctcg ggatctcggt ctcgtacgag ccgacgggga
     2701 gcgggacctg ggccgcgctc gtcgaagtcg cggtcgccgg agcgcgcacg ggtgccaaat
     2761 cgtttccgat tcgaacgtca tacgaggaag tacagcgccg ctcggcactc ttcgcggccg
     2821 aggtcctgcg atccgcgttg atcagcgatt gagcgcgatc cacttgtcga ggatgacctg
     2881 ctggcgcgcc agcatcgctg cccggtcggc atccgccgtg tcgtcgtaat cgagcaagtg
     2941 cagcatcccg tgcagaacca ggaaacccag ttccttgctc agcgagttgc cctgatcgtg
     3001 cgcgttgtcc gaagcaaccg tgacgcaaat cgcgatgtcg ccgcctgaaa agtccgggtc
     3061 gtcgtatggg aacgtgatga tgtcggtcgg cgatgggtcg cccatgaact ggtcatgcag
     3121 cagcccgatc tcccgctcat ccacgaacgc gatagcgact tgccacggcc cggacgcgtg
     3181 ctcctccgac aggcagaagg taacgagatc ggagatgcgt tcctcggtga cggcatccgg
     3241 caccggagtt ttacggatga cggtgacgtc ggccgtgatt ccgtgtgcag acactacgca
     3301 ccgccggcga gcgcttgccg gaccgcgtcg ctgacccgct tgccgctggc gcgatcccca
     3361 gccgccttca gcgcgaccgg catcaccttg cccatctctt tcggactgct tgccccgacc
     3421 cgagctattg cgtcccggac cagtgcggca agctcgtcat cgctcatctc ggtcggcata
     3481 tagcgcttga gcacctcgag ctgggcagac tcgcggtcga ccagatcggc ccgtcctgcc
     3541 gcgcgatatt gatcgatcga atcgccgcgc cgcttggcct gcgtcttgag gacatccatc
     3601 tcctcggacg gcgacaatgc cgatcgcttg tcgatctccg cgttcttgag cgcggcgagc
     3661 gtgaatcgca acgtctcccg ggcggtgacg tccccggcct tcatcgcgtc cttgagatcg
     3721 gcctccatgc gggccttgac cgaatccgtc atcgattaca ccccaaattg cagcttgcgg
     3781 ccgccgagaa cgtggaagtg caaatgcgcc acttcctggc caccatccgg tccatcactg
     3841 gtcacgaccc gaaatccgct caggtcgata tctttgatcc gcgccacttc gttgcaaaga
     3901 aggaggcaat cggccagcat ctcgcggtcc gcacccgtcg cctcatgcag cgcggcgatg
     3961 tgctccttcg gaatgacaag ggtgtggacc ggcgccttcg gggcgatgtc ctcgaacgcg
     4021 atcgcccgat cgctttcggc cacccgcgat gacggaatct caccgcgtac aattttgcag
     4081 aagatgcagt cgtccattgc ctgccctgtc cacgatctcc cgaattcgcc cgctgccggc
     4141 gacaccggca atcctaccag ccaccatgcc cgaactccct gaagtc
//
LOCUS       DATAOZ010000351         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3300557, whole genome shotgun sequence.
ACCESSION   DATAOZ010000351 DATAOZ010000000
VERSION     DATAOZ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3300557"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1658)
                     /locus_tag="VHR64_08995"
     CDS             complement(156..1658)
                     /locus_tag="VHR64_08995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX3303003.1"
                     /translation="MFIPRSESKYGAALPIVGLCILAITLRLCSTLTLSHFYDAVSHE
                     TDRITIQESLIRQIERYQRVEESMIAARSLPVVPLDAVIAGTNQSISDLAVREPDDAD
                     QVRPLFEQYAADLRAEADLIANGQIDAAIALRQSGSLASGSAVHERLTALHESASARK
                     AEAQRKVKVMLIVPTALGSVFVCTVFITYDQRRKRAYSLLAAQETRFRSLIQQSTDIV
                     LVADIHGAITYATPAAEHAFGRSAAQLRNTELADFVLPDDRGAVTDLILEAIARDGQP
                     SRGEVRFLLSNRVPRTMEVTAVNLIENEAVQGIVITAHDITERKMLLESLRHQAHHDS
                     LTGLPNRLVLEDRLDSAIRQAQSTGVPTSVLFIDLNDFKMINDRDGHAMGDELLVAAA
                     DRLRETLRIDDTLARIGGDEFVVLLDGMDRDYAVRVAERLTEALAELPVRERILGQGA
                     SIGITVIRDNELPADIVLKHADSAMYAVKATGKTGWHVFGEPVPGLISVA"
     gene            1862..>2106
                     /locus_tag="VHR64_09000"
     CDS             1862..>2106
                     /locus_tag="VHR64_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX3303004.1"
                     /translation="MPVVAAADLRVLMRDLLIGIGTPGDVAGMVGDSLVDANLAGHDS
                     HGVIRILHYLEMCEAGEVFPAAEPEIIREHGATVTIDG"
BASE COUNT          367 a          683 c          677 g          379 t
ORIGIN      
        1 ccggtcacgc gagaggagct ggagctggcc ctgaccgcgc tcggcaaccg gatcaccgcg
       61 caccttggcg gcggtcatcg catcctcatc cttgaccgtg aaaatcggga agcagaactg
      121 atcgagctga gcccggagct cctgtagcgc cggcgtcagg cgacggagat cagccccggg
      181 accggctcgc cgaacacgtg ccatcccgtc ttgccggttg ccttgaccgc gtacatcgcg
      241 ctgtcggcgt gcttcaggac gatgtcggcc ggaagctcgt tgtcccgaat cacggtgatg
      301 ccgatgctgg cgccctggcc aagaatccgt tcacgcaccg gcagctcggc gagtgcctcg
      361 gtgagccgct cggccacgcg cacggcgtag tcccggtcca tgccgtcgag cagcaccacg
      421 aactcgtcgc caccgatccg ggccagggtg tcgtcgatcc gcagcgtctc gcgcagccgg
      481 tccgccgcgg ccaccaggag ctcgtctccc atcgcgtgac cgtcgcggtc gttgatcatc
      541 ttgaagtcgt tgagatcgat gaacagcacg gaggtcggca cgccggtcga ttgcgcctgc
      601 cgaatcgcgc tatccagccg gtcctcgagc accagccgat tcggcaatcc ggtcaggctg
      661 tcatggtgcg cctgatgccg cagtgattcg agcagcatct tgcgctcggt gatgtcgtgc
      721 gcggtaatga cgatgccctg aaccgcctcg ttctcgatca ggttgaccgc cgtaacttcc
      781 atcgtgcgcg ggacacggtt gctgaggagg aagcgcacct cgccgcggga aggctgtcca
      841 tctcgcgcga tcgcctcgag gatcagatcg gtcaccgcgc cgcgatcgtc cggcaggacg
      901 aagtcagcca gctcggtatt gcgcaattgg gcggccgacc gtccgaacgc gtgctcggcc
      961 gccggggtgg cataggtgat cgcgccgtga atgtcggcca cgaggacaat atccgtgctc
     1021 tgctgaatca gtgagcggaa gcgcgtctcc tgcgcggcaa ggagagagta ggcccgcttg
     1081 cgccgctggt cataggtgat aaagaccgta cagacgaaga ctgagcccag ggcagtcgga
     1141 acgatcaaca tgaccttgac cttccgctgc gcttcggctt ttcgcgcact cgccgactcg
     1201 tgcagggcgg tcagccgttc gtggacagcc gatcctgacg caagcgagcc ggattggcgg
     1261 agagcgatgg ccgcgtcgat ctgcccattc gcgatcaggt ctgcctccgc gcgcagatcg
     1321 gccgcgtact gctcaaacag cggccgcact tgatcggcgt cgtcgggttc ccgcacggcc
     1381 agatcggaga tcgattgatt cgttcccgcg atcacggcgt cgagcggaac aaccggaagg
     1441 gagcgggcgg cgatcatgct ctcttcgacg cgctggtacc gctcgatctg ccggatgagc
     1501 gactcctgga tcgtgatccg gtcggtttca tggctgacgg catcgtaaaa gtggctcagc
     1561 gtcagggtgg aacagagccg gagggtaatc gcgaggatgc acaacccgac gatgggcagc
     1621 gctgctccgt atttcgactc cgagcgcgga atgaacatca agaccacttc tgaatgacat
     1681 gcccgccaca gcaaaccggt gactctcgca caatatcgtc agcctcggtt gcaaaacagg
     1741 gatcgacgtg gttctccagt accgctttcc cggccccgtc gggactattg atcgccggct
     1801 caccgggacc ctcccgtttc gtatgcttcc ggcaggaacc gccaccgttg ggagatcacc
     1861 gatgcctgtt gtcgccgctg ctgaccttcg cgtcctgatg agggatctct tgattggcat
     1921 cggaacgccc ggggacgtgg ccgggatggt cggcgactcg ctggtcgacg ccaatctcgc
     1981 cgggcacgac tcccacggcg tcatccgcat cctccattac ctggagatgt gcgaggcggg
     2041 cgaggtcttc cctgccgccg agcccgagat catccgcgag cacggcgcga ccgtcacgat
     2101 cgatgg
//
LOCUS       DATAOZ010000352         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5931843, whole genome shotgun sequence.
ACCESSION   DATAOZ010000352 DATAOZ010000000
VERSION     DATAOZ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5931843"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHR64_09005"
     CDS             <1..198
                     /locus_tag="VHR64_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303005.1"
                     /translation="SSAPIVVLQTSDRGAGSVHIDFTAPTNLDRTAFDVMFRLVDSLT
                     TPCSDLFSHDPHPGTAGAHVR"
     gene            404..1699
                     /locus_tag="VHR64_09010"
     CDS             404..1699
                     /locus_tag="VHR64_09010"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3303006.1"
                     /translation="MPTPIARLVPVLLVMGASVATTQLAPRGDPMPMLNAAAQSTPLA
                     PRRVIAPPGPKPVGPYSPGIMVGDFLYVSGQGARDRDGRLPDTVEGQVKQTLNNVKGI
                     VEAAGLTMEHVVYAQVYLINMSNSDTMDRVWREFFPKQPPARAVLGVHRMPTDTPVEI
                     NVVAVRDLSGKKSIVPAGYPANSPATAGVMAGDRLYLSGSFGADLGTGSVPTDPAAQV
                     QLALNNMKRTLDAAGLDFRHVVFVNPYLTDQASGQMNAIYAKHFEFGNTPARATIRVA
                     SLPGGNTIEFTGVAVGDLSKRLAVRPKNMRPSATASPCVFAGDTYFCSAKGPFTPGPD
                     KVQGIYAATVEAQVRQTMRNLLDGLEEAGLTLANVVATNVYLDDIEDFSRMNRVYAQY
                     FPELFPTRTTVAPLPPIERKPVNEDIFPGLEQISVVAVK"
BASE COUNT          353 a          619 c          569 g          346 t
ORIGIN      
        1 agttccgcgc caatcgttgt gctgcagacc tccgaccgag gagctggatc agtccacatc
       61 gacttcaccg cgccgacgaa tctcgaccga acagcgttcg acgtcatgtt tcgacttgtc
      121 gacagtctga ccactccgtg cagtgacctc ttctcgcatg atccccatcc tggaaccgcg
      181 ggagcccacg tccgctgaac accgcgaccc ggtttttagg aatcgtgtgc gcttcacctt
      241 ctgcggcacc aaccggtaac gcatttgatc tgattggctg ctcgtcgtct cgttggcggc
      301 cgcaccgggc gcaaccggga gcaacctctc cgatctcgca ccctcgggta acgccggcga
      361 gagcgcttat aattcccacc gacgttagcg tatggatacg atgatgccaa cgccgattgc
      421 acgcctcgtt ccggtactgc tggtgatggg cgcctcggtc gccacaaccc agctggcgcc
      481 ccgtggcgat ccgatgccga tgctcaatgc cgccgcgcag tcaactcctc tcgcaccgag
      541 acgggtcatc gcaccccctg gaccaaagcc ggttggcccc tatagtcccg gcatcatggt
      601 cggcgacttc ctctatgtct ctggccaggg cgcacgcgac cgcgacggaa ggctgcctga
      661 cacagtcgaa gggcaggtga aacagaccct aaacaacgtc aagggcatcg tcgaggccgc
      721 cggcttgacg atggagcacg tcgtttatgc ccaggtgtat ctcatcaaca tgtccaactc
      781 cgacacgatg gaccgggtct ggcgcgaatt ctttcccaaa cagccgccgg cacgtgccgt
      841 gctcggggtg caccggatgc ctaccgatac tccggtcgag atcaatgtcg tcgccgtccg
      901 cgatctctcc gggaaaaagt cgatcgtccc tgccggctat ccggcgaact cgccggcgac
      961 ggccggcgtg atggcgggcg accgtctcta cctgtccggc tcctttggcg ccgatctcgg
     1021 aaccggcagc gtgcccacgg atccggcagc gcaggtgcag cttgcgctga acaatatgaa
     1081 acggacgctg gatgcggcgg gcctggattt ccgtcacgtg gtgttcgtca acccttacct
     1141 gaccgaccag gcatcgggac agatgaacgc catctacgcc aagcacttcg aattcgggaa
     1201 cacgccggcg cgcgccacga tccgtgttgc cagcctgccg gggggcaaca ccatcgaatt
     1261 caccggcgtg gcggtcggcg atctctcgaa gcggctcgcg gtccgaccga agaacatgcg
     1321 gccgagcgcc actgccagcc cgtgcgtctt tgccggcgac acctacttct gttcggcgaa
     1381 ggggccgttc acgccgggac cagacaaggt gcagggcatt tatgccgcga ccgtcgaggc
     1441 gcaggtccga cagacaatgc ggaacctgct tgatggcctc gaagaggccg ggctgacgct
     1501 cgccaatgtc gtcgccacca acgtgtacct cgacgacatc gaggactttt cgcgaatgaa
     1561 ccgcgtctac gcgcaatact tcccggagct gttcccgacg cggacgacgg ttgccccgct
     1621 tccgcctatc gagcggaagc cggtcaacga ggatatcttt cctggcctcg agcagatctc
     1681 ggttgtcgct gtgaagtaaa agtaggaatc ggtgaacaag gaggacgaag tggacctcaa
     1741 cggtctgaca cggcgggaat tgttcctgat gggcaatgcc ctggcggtgc cgacgctgct
     1801 cgggcgactt gacgcgccgc tcgaggccgc gccggaggcc ggaccactgg gggccggacc
     1861 acagatctat gagtcgattg gtgtcga
//
LOCUS       DATAOZ010000353         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7512047, whole genome shotgun sequence.
ACCESSION   DATAOZ010000353 DATAOZ010000000
VERSION     DATAOZ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7512047"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1640)
                     /locus_tag="VHR64_09015"
     CDS             complement(42..1640)
                     /locus_tag="VHR64_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX3303007.1"
                     /translation="MTDQTRPSTLCATAIAMCRRRLRATKISDTTGASLTGGELLTRA
                     LVLRRLLRRSVLAPDEQNVAVLLPPTAAAVVTNFALTLDNRVVVNLNYTLSSDLLNSS
                     IAQAGVRHVLTSRAFLERVPLQLDAELIFLEDFRTAATRTDKIVSAALAFGMPARLLT
                     RLLGVHKHQSDDVISIMFTSGTTGDPKGAVLTHGNIETNVRGVDEVIHIDPHDVLIGV
                     LPFFHSFGYAITLWSVLCLDLAAAYHVNPLEAKLIGRLCRERKGTILLATPMFLRTYI
                     RRCEPEDFATLEVVITGAERLPAQVADDFEAKFGLRPVEGYGTTETSPLISANIPPNR
                     APGPPEHSARAGTVGKPPPGVQVKVVDPESGADLGPDDQGMLLVKGPNVMRGYLNRPD
                     ATVAALRDGWYVTGDICVIDEDGFIRIVGRESRFAKIGGEMVPHGLVEEALTAIVGVD
                     DEGMQRVVVVSVPDSERGERLVVVHTPLEQTPAELRAALNAAGLPNLFIPDGNSFLCV
                     DRLPALGTGKLDIRAIKRLAEGRV"
     gene            complement(1637..>2068)
                     /locus_tag="VHR64_09020"
     CDS             complement(1637..>2068)
                     /locus_tag="VHR64_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303008.1"
                     /translation="IPLLDRIYLSKQIPAGETLRAVHLGHHHSLDGYYFMFAALILSR
                     IVRRDLTSRLASILRGYFGLMFSYGLLNMAEDFWLEQIVKRGWTDQELPDFLMPALSV
                     GWALIIVGAIISWALLFRPIVADQRPAARRTRLAAGAHTPA"
BASE COUNT          364 a          665 c          720 g          319 t
ORIGIN      
        1 aaccccgaac cccgacgccc ccaaaaccca aaacccaaaa cctaaaccct accctcggcc
       61 agtcgcttga tcgcgcggat gtcgagcttg ccggtgccga gggcggggag ccggtcgacg
      121 cagaggaagc tgttgccatc ggggatgaag aggttcggca ggccggctgc attcagggcg
      181 gctcgcagct cggccggggt ctgctccagc ggcgtgtgga ccacgaccag ccgctcgccc
      241 cgctccgaat ccgggaccga gacgacgacc acccgctgca tgccctcgtc gtcgaccccg
      301 acgatcgcgg tcaatgcctc ctcgaccaga ccgtgcggga ccatctcgcc gccgatcttg
      361 gcgaagcggc tctcccggcc gacaatccgg atgaatccgt cctcgtcgat tacgcagatg
      421 tcgccggtca cgtaccagcc gtcgcgcagg gcggccacag tcgcatccgg gcggttcagg
      481 tagccacgca tcacgttcgg ccccttgacc agcagcatcc cctggtcgtc cggcccaaga
      541 tccgcgccgc tctcgggatc gaccaccttg acctgcacac ccggtggcgg cttgccgacg
      601 gtgccggccc gggctgagtg ctccggcggt ccgggcgcgc ggttgggcgg aatattggcc
      661 gagatcagcg gcgaggtttc ggtcgtaccg tagccctcga ccgggcggag accgaatttc
      721 gcctcaaagt cgtcggccac ctgtgcgggc agccgctcgg cgccggtgat gaccacctcg
      781 agcgtggcga aatcctccgg ctcgcagcgc cggatgtagg tgcgcaggaa catcggcgtg
      841 gcgagcagga tcgtgccctt gcgctcccgg cagaggcggc cgatcagttt cgcctcgagc
      901 gggttgacgt ggtaggcggc ggcgagatcg aggcagagca cgctccagag cgtgatcgcg
      961 taaccgaatg aatggaagaa gggcagcacg ccgatcagga cgtcgtgcgg gtcgatgtgg
     1021 atcacctcgt cgaccccgcg gacgttggtc tcgatgttgc cgtgcgtgag gaccgcgccc
     1081 ttgggatcgc cggtcgtgcc ggaggtgaac atgatgctga tcacgtcgtc ggactggtgc
     1141 ttgtgtacgc cgaggaggcg agtcaggagc ctggccggca tcccgaaggc gagcgcggcg
     1201 gaaacgattt tgtcggtgcg ggtggcggcg gtccggaagt cttcgaggaa gatgagctcg
     1261 gcgtcgagct ggagcggcac gcgctcgagg aaggcccggc tggtcaggac gtggcgcacg
     1321 ccggcctgcg cgattgacga gttgagcagg tcggagctga gcgtgtagtt gaggttgacg
     1381 accacccggt tgtcgagcgt cagggcgaag ttggtcacga ccgccgcagc cgtgggcggc
     1441 agcagcacgg ccacgttttg ctcgtcgggc gcgagcaccg agcggcggag caagcggcgc
     1501 aggaccagcg cgcgggtaag caactcccca ccggtcaggg aggccccggt cgtatcggag
     1561 atcttggtcg cgcgcaaccg ccggcggcac atcgcgatcg cggtcgcgca gagcgtggac
     1621 ggtctggttt ggtcggtcat gccggcgtgt gcgctcccgc tgcgagcctg gtcctgcgcg
     1681 cagccggccg ttgatcggct acgatcggcc ggaacaacaa cgcccaggag atgattgctc
     1741 cgacaattat caacgcccag ccaaccgaaa gcgccggcat gaggaaatcg ggcagctcct
     1801 ggtcagtcca gccccgcttg acgatctgct ccagccagaa atcctcggcc atattgagga
     1861 gcccgtacga gaacatcagg ccgaagtagc cgcgcaggat cgaggccagc cggctagtca
     1921 ggtcgcgccg cacgatccgg ctcaaaatca gcgccgcaaa catgaagtag tagccatcga
     1981 ggctgtggtg atgaccgagg tggacggccc gcaacgtctc gccggccggg atctgcttcg
     2041 acaggtaaat ccggtccagc aacgggat
//
LOCUS       DATAOZ010000354         9690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6721139, whole genome shotgun sequence.
ACCESSION   DATAOZ010000354 DATAOZ010000000
VERSION     DATAOZ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6721139"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..728
                     /locus_tag="VHR64_09025"
     CDS             135..728
                     /locus_tag="VHR64_09025"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEX3303009.1"
                     /translation="MSRHTVEARKGAGYKLEAGQRFKVINTHGHQVVDFWAISTEDPT
                     EFMSMVHTRSTLTKLVPQTGDILYSNVRAPIVRFVEDTSPGVHDTVVAPCDLPRYHLI
                     GVEGFHDSCSNNFMTVMKQYDISEYRQAPESFNLFMNIPWTEDGKLSFEPTVSKPGDY
                     VVFEAIRPVLMVVSSCPQDILPINSGNPVEFDVEISD"
     gene            complement(718..2163)
                     /locus_tag="VHR64_09030"
     CDS             complement(718..2163)
                     /locus_tag="VHR64_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX3303010.1"
                     /translation="MANAYAAVDLGNSSGRVMLGRISDDRIETTEVRRIPNAPIRHQD
                     GWHWDVHRLFDEALLGLRQAAEQTPLDGIAIDTWGVDYGLLGADGTLLDDPYSYRDER
                     TLPMVALGEDRLAAERLYALTGCQAGPINTLYQLLADQHTGRLDRAQQFLLTPDLLAY
                     WLTGEIGADETIASTTQLFDPNTRDWSWDAIDAMQLPRRLFAPVRRTGTVLGTVRPVF
                     GLPPGIPVITAAGHDTAAAFVAATGGTDQLVISIGTWSLVGVELTGPLITEAGWEANF
                     TNELGVLGTIRFLRNAAGLWIIQECLREWQGKGENVDWAYLIDLAFESQSFASLFNPD
                     DRRFMEPGPMADRVMDAGDEPLDGSHGPIVRSVLESLSMNHRWLLETVEGVIGRTLSR
                     IRIVGGGSQNWLLCQMIADVTGREVVAGPAEASAIGNIAMQAIATGQIDDLAIARDLI
                     DRSFPARTYEPNPDHAMWDAEYERWRALANR"
     gene            complement(2385..2672)
                     /locus_tag="VHR64_09035"
     CDS             complement(2385..2672)
                     /locus_tag="VHR64_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303011.1"
                     /translation="MEQDPWRRDEPPGDAGEPPLTGEVIDAGSRMETPGWESGYPFRN
                     REGVGGWLFGPRSFRGGRIQVYGCSPGCIILSCIVSLVASILLTLLLNWIF"
     gene            complement(2697..3707)
                     /locus_tag="VHR64_09040"
     CDS             complement(2697..3707)
                     /locus_tag="VHR64_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX3303012.1"
                     /translation="MRGRIDERVVTIGGGTGSYTMLSELKRVVNRLWAVVAMTDSGGS
                     SRRLMDQFGHPLPLGDLRQALVALSRSSRLWSDLFRYRFPDAPDGGISGHSLGNLILH
                     ALEDLNDGELLGALEDAQDLLDTAGQVLPVTLQQSTLCAYLSDGSVVRGETEIDRPRD
                     RSVAPIERVCLDPPVKVLPEVRKALTRADKILIGPGDLYTSIVPCLLVAGVGDAIQSC
                     DGEVIYICNVMTKRGETDGFRASDFVRVIHHYLGRRVDTVVINTATPSAELAARYADE
                     GAELVVPDLDVLRTMVPQVRADRFAITDPLMRHDAERVVLGIWPELMADPASGAGRTS
                     AD"
     gene            3808..5214
                     /locus_tag="VHR64_09045"
     CDS             3808..5214
                     /locus_tag="VHR64_09045"
                     /inference="COORDINATES: protein motif:HMM:NF005686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3303013.1"
                     /translation="MSREEIVFSSAKALARRIRDREISAREVMIAHIEQIERVNPTVN
                     AIVSMLNPEVAIERANEADRQPCEGPLHGLPIAFKDLEDAVGFPNTQGSPIFADFYPE
                     QDSVIVERMKKAGAIPIGKTNVPEFGLGSHTYNNVYGPTRNPWNLDRTAGGSSGGAAV
                     ALATGMLPIADGSDMGGSLRNPGNFNNVVGFRVSAGLVPKSPSSMPWLPLSVKGPLAR
                     SVEDVGFFLSAMAGFDPRDQLSYPVDASAFADDLTIDPKHLRIAWSTDLGGLPVDPAV
                     RSVVDGARAMFEAIGCQVEDACPDFSDADEIFKNLRAFSLTASAELMRSHGEQMKPEA
                     IWNIESGLKLSAEDVGRAMVKQGALFHRTSAFMEHYDAFVCVVNQVPPFPIDLDWPHE
                     IDGVQMETYIDWMKSAYFVTATRMPAISVPAGFTPDGLPVGIQIVGKYRGDLALLKIA
                     NAFEQATNIGSRRPPIVQ"
     gene            complement(5577..6671)
                     /locus_tag="VHR64_09050"
     CDS             complement(5577..6671)
                     /locus_tag="VHR64_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX3303014.1"
                     /translation="MYTSLVGHPLSDVDTPALVIDLDALDHNIATMAADIAARGAQWR
                     PHSKANKTPAIVHKEIAAGAIGVTCAKVSEAEVMAAAGIRDILIANQVVGPIKTRRLA
                     WLAREADVIVAVDNPDNVRELDCAAGQAGTKPRILIEVNSGMNRCGTLPGGPTVELAK
                     LIDSMPNLRFAGVMAWEGHSMAINDPAEREAEIRRACGSLVDTANACRAAGLDVQIVS
                     AGGTGTYLVSAGVEGITEVQAGGGIFGDSTYRDLGANVIPALSIMAQVTSRPTPEKVM
                     IDAGRKSIDPSARQPSVEGTETTGDMSFSAEHGRFTLATPSDCPRIGDRVFLQSGYSD
                     QMCHLHEYFIGVRNGIVAAVWPIQGRGRLQ"
     gene            complement(6752..7711)
                     /locus_tag="VHR64_09055"
     CDS             complement(6752..7711)
                     /locus_tag="VHR64_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303015.1"
                     /translation="MIAAGWPLGQAANADTATDGWRLVGLIAITLATIVVLALIFRPW
                     TSTEGAPGGQPAPDGGEREPGVPDTPTGIHPVPTAPTIQTDAELAAERLRQIVQQGRI
                     DRRRPSGTGIPQGGRPTGEEASKEGAMETGEQGTIPGMEAEQGGLDAEALEEQLRRLF
                     GPQGEPGKIVPFTPRRPPTPDDQERPRVPGAEPRITRFPVDVATRDGITASIQELLFC
                     SNVGELLHGFALYTDRYLFQFMDESRMSEDEFREAFSDVAAKDPSNWTRIDSISDFTR
                     LDDGRVTVRVRYIDGSMIDGTEQFTMRYDPRLERWLIDDIQAI"
     gene            complement(7708..8091)
                     /locus_tag="VHR64_09060"
     CDS             complement(7708..8091)
                     /locus_tag="VHR64_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303016.1"
                     /translation="MGRFFLTLAFLMLVLLLIDSGMNDPDVQPVVDGQVRRVRHQIEA
                     ISSWVHTSPIFPWGDDPDSASASAAALSGLHDGHSWSWYWSTMLAMAVAALSLAKSVS
                     KEISRTRAAATLPPRVGVRPAGRRP"
     gene            complement(8538..>9690)
                     /locus_tag="VHR64_09065"
     CDS             complement(8538..>9690)
                     /locus_tag="VHR64_09065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX3303017.1"
                     /translation="VTFAERLVVENVLSAVEHGAQVVTHATVTGIVSEGAVVRGVEVR
                     DTLTGAQHRIAGRVVVNVAGPWVDQVLAGAPKGRVHDTFIGGTKGSHIIVDRFEGAPT
                     DAIYYESRSDHRPILVIPFNGMIMLGSTDIRYDGALDRVEASDAEVDYLLSEANALFR
                     GVNLTPDDVRYSYAGIRPLPRTAKGSAGAITRRHMIKDHAPSLRGLWSVIGGKLTTHR
                     SLAEEFVDKALRALGRDSKSRTASVPLPGAAGVAFGTYRQCIVRPAAQLGVGDRSAYR
                     LVDLYGTRAEEILALIPSAPALATVVDSYSGAIAAEAAFAVQEEMALSLADILLRRTM
                     IAYGPHAGIGPDERVLEVAGKELGWTKPRQLKELAAFREWIQRYRPRAG"
BASE COUNT         1687 a         3314 c         3057 g         1632 t
ORIGIN      
        1 cccgatggac ctctcgccgg tcaacagcgg gaacccggtc gacttcgcag tcgagatcag
       61 cggctgaccc ggcgtccggc gtaccatgtc gccatcgtca aattcggcgc gcaggcagac
      121 aaaggagagc gctggtgagc aggcacacgg ttgaggcgcg gaagggcgcg ggatacaagc
      181 tcgaggccgg tcaaaggttc aaggtcatca acacccacgg ccatcaggtc gtcgatttct
      241 gggcaatctc gaccgaggac ccgaccgagt tcatgtcgat ggtccacacc cgctcgaccc
      301 tgacaaagct cgttccgcag accggcgaca tcctctactc gaacgtccgc gcgccgatcg
      361 tgcgtttcgt cgaggacacc agcccgggtg tgcatgacac tgttgtcgcc ccgtgcgatc
      421 tgccgcgcta ccacctgatc ggtgtcgagg gattccacga ctcctgctcg aacaatttca
      481 tgaccgtcat gaagcagtac gacatcagcg aataccggca ggcgccggag tcgttcaacc
      541 tcttcatgaa catcccgtgg accgaggacg gcaagctcag cttcgagccg accgtctcca
      601 agccgggcga ttatgtcgtg ttcgaggcga tccggccggt gctgatggtc gtgtcgtcct
      661 gcccgcagga catcctgccg atcaacagcg gcaacccggt cgagttcgac gtcgagatca
      721 gcgattagcg agcgcccgcc agcgctcata ctccgcatcc cacatcgcgt ggtccgggtt
      781 cggctcatac gtccgagccg gaaatgagcg atcgatcaga tcccgggcga tcgccagatc
      841 gtcgatctgc ccggtcgcga tcgcctgcat cgcgatgttg ccgatcgccg atgcttcggc
      901 cgggccagcc acgacctccc gccccgtcac atcggcgatc atctggcaga gcagccaatt
      961 ctgcgaaccg ccgccgacga tccggatccg gctcagcgtc cgcccgataa caccctcgac
     1021 cgtctccagc agccagcggt ggttcatcga gaggctctcg agcacgctcc gcacgatcgg
     1081 cccgtggctg ccgtcgagcg gctcgtcgcc ggcatccatc acccgatcgg ccatcggtcc
     1141 cggctccatg aaacggcggt cgtccggatt gaacagcgac gcgaatgact gcgactcgaa
     1201 ggccagatcg atcaaataag cccagtcgac gttctcgccc tttccctgcc actcccgcaa
     1261 gcactcctgg atgatccaga gaccggcggc gttgcgcagg aagcggatcg tgccgagcac
     1321 gccgagctcg ttggtgaagt tggcctccca ccctgcctcc gtgatcaatg gcccggtcag
     1381 ctcgaccccg accagcgacc aggtcccgat cgagatcacg agctggtcgg tgccgccggt
     1441 tgcggccacg aacgccgccg cggtgtcatg tccggccgcg gtgatgaccg ggatgcccgg
     1501 cggcagtccg aaaacggggc ggacggtgcc gaggactgtt ccggtgcggc gcaccggcgc
     1561 gaacaggcgg cgcggcagct gcatcgcgtc gatcgcgtcc cagctccaat ctcgcgtgtt
     1621 cggatcgaag agctgggtcg tcgaggcgat cgtctcatca gcgccgatct cgccggtaag
     1681 ccaatacgcg agcagatcgg gagtaagcag gaattgctgg gcacggtcta accggccggt
     1741 gtgttgatcg gccaggagtt gatagagcgt gttgatcggc cccgcctggc atccagtaag
     1801 cgcgtaaagc cgctcagccg ccagtcggtc ctcgccaagc gcgaccatcg gcaaggttcg
     1861 ctcgtcgcgg tacgaatacg gatcatcaag cagcgtgccg tcagctccaa ggaggccgta
     1921 atcgacgccc cacgtatcga tggcaatccc atccagcggc gtctgctccg ccgcctgccg
     1981 caatccgagc agcgcctcgt cgaagagcct atggacgtcc cagtgccagc cgtcttggtg
     2041 ccggattggc gcgttcggaa tccggcggac ttccgtggtc tcaatccggt cgtcgctgat
     2101 gcggccgagc atgacccggc cgctcgagtt cccgagatcc accgccgcgt atgcattagc
     2161 catcgccccg tcccggtcgc tcaaatgccg tcgatatgcg agatggtagg gcctacaagc
     2221 gatcccgcgc acgtaatttc gacgtagcgc cggccccccg tggccggctt tgggtcgacg
     2281 gacggtgccg tcgatcgagc acatccggcg tcaggtaacg atgcgctcgg caccaaggcg
     2341 gcccagaggc gggccgcgca gcgccattca cgggcgaaaa cgggtcagaa aatccagttc
     2401 agcaggagcg tcaacaggat cgaggcaacc agcgagacga tgcacgacag gatgatgcag
     2461 ccgggggagc agccgtagac ctgtatccgg ccaccgcgaa atgagcgcgg cccgaaaagc
     2521 cacccgccga ccccctcccg gttgcgaaat ggatacccgg attcccatcc aggggtctcc
     2581 atccggctcc ccgcatcgat cacttcgccg gtcagcggtg gctcgcccgc gtctcccgga
     2641 ggctcatcgc gtcgccacgg atcttgctcc atcgcactaa cctcgatccg ctcaggtcaa
     2701 tcggccgagg tgcgaccggc cccagaggcg ggatcagcca tgagctcggg ccagatcccg
     2761 agcaccaccc gctccgcgtc gtgccgcatc agcgggtccg tgatggcgaa ccgatcggcc
     2821 cgcacttgtg gaaccatcgt ccgcaacacg tcgagatcgg gcacaaccaa ctccgctccc
     2881 tcgtccgcat atcgcgcggc cagttccgct gatggcgtgg cggtgttgat cacgaccgta
     2941 tcgacgcgcc ggccgagata gtggtgaatc acccgcacga agtccgaggc ccggaagccg
     3001 tcggtctcgc cgcgcttggt catcacgttg cagatgtaga tcacctcgcc gtcgcacgac
     3061 tggatcgcat cgccgactcc cgcgaccagg aggcatggca cgatgctggt gtagaggtcg
     3121 cccggcccga tcaggatctt gtcggcccgg gtcaacgcct tgcgcacttc cggcagaacc
     3181 ttgaccggcg gatcgaggca aacccgctcg atcggcgcga ccgagcgatc tcggggtcga
     3241 tcgatctcgg tctccccgcg cacgacggag ccgtcgctga gataggcgca gagcgtcgat
     3301 tgctggagcg ttaccgggag gacctgaccc gcagtgtcga ggagatcctg ggcgtcctcc
     3361 agcgcgccga gcagctcgcc gtcgttgaga tcttcgaggg cgtgcaggat caggttgccg
     3421 agcgagtgcc cactgatccc gccgtccggc gcatcgggaa accggtagcg aaagaggtcg
     3481 ctccagagcc ggctcgaccg cgacagtgcg accaacgcct gccgcagatc gcccagcggc
     3541 aacgggtggc caaactggtc catcagccgg cgcgacgacc cgccgctgtc ggtcatcgcc
     3601 acaaccgccc agagccggtt cacgacccgc ttcagctcgg acagcatcgt gtacgagccg
     3661 gtgccgccgc caattgtcac gacgcgctcg tcgattcggc ctcgcatgcc aggtccctgt
     3721 gtccgcagcc gcaaatcccg cccgggcaga atgcgcggat cggcttaagc taccgcagat
     3781 actagcgcgt catcagaaag gcagatcgtg tccagggaag agatcgtatt cagctcagcc
     3841 aaggccctcg ctcgccgcat ccgggatcgg gagatctccg cccgcgaggt gatgatcgcg
     3901 cacatcgagc agatcgagcg agtcaaccca acggtcaatg cgatcgtctc gatgctcaat
     3961 ccggaggttg cgatcgaacg cgcgaacgag gccgatcgcc agccgtgcga aggtccgctt
     4021 cacggcctgc cgattgcctt caaggatctc gaggacgccg tcggcttccc gaacacgcag
     4081 ggctcgccga tcttcgccga tttctatccg gagcaggact cggtcatcgt cgagcggatg
     4141 aagaaggccg gagcgatccc gatcggcaag acgaacgtgc ccgagttcgg cctcggctcg
     4201 catacgtata acaatgtcta tggcccgacc cgcaacccct ggaacctcga ccgcacggcc
     4261 ggcggcagct cgggcggcgc ggcggtcgcg ctggcaaccg gcatgttgcc gatcgccgac
     4321 ggcagcgaca tgggcggctc gcttcgcaac ccgggcaact tcaacaacgt ggtcggcttc
     4381 cgggtcagcg ccggcctggt gccgaagtcg ccctcgtcga tgccctggct gcccctcagc
     4441 gtcaaaggac cgctggcccg ctcggtcgag gacgtcggct tctttctctc ggcgatggcc
     4501 gggttcgacc cacgagacca gctctcctac ccggtcgatg cgtcagcgtt cgcggacgac
     4561 ctgacgatcg atccgaagca cctccgaatt gcctggagca cggatctcgg tggcctgccg
     4621 gtcgatcccg ccgtccgaag cgtggtcgat ggcgcccggg cgatgttcga ggcgatcggc
     4681 tgccaggtcg aggatgcctg ccccgacttc tcggacgccg atgagatctt caagaacctg
     4741 cgcgccttct cgctgaccgc ctccgccgag ctgatgcgaa gtcacggcga gcagatgaag
     4801 ccggaagcga tctggaacat cgaatccggc ctcaagctct cggccgagga cgtgggcaga
     4861 gcgatggtca agcagggcgc gctcttccac cgcacgagcg ccttcatgga gcattacgac
     4921 gccttcgtct gtgtcgtcaa ccaggtgccg cccttcccga tcgacctcga ctggccacat
     4981 gagatcgacg gcgtgcagat ggagacctac atcgactgga tgaagtcagc ctactttgtc
     5041 acggcaaccc ggatgccggc gatctcggtc ccggcgggct tcacccccga tggcctgccg
     5101 gtcggcatcc agattgtcgg caaataccgc ggcgacctcg cgctgctcaa gatcgccaac
     5161 gccttcgagc aagcaacgaa catcggctca aggcgcccac cgattgttca ataggcggga
     5221 ggcgagcagt tggggcacgg aggcgaaacg tagggatttg ccgtggcaaa cccagcgccc
     5281 atcgctcgga gcgtcgcatg gcaatgcgac ctcatgatgt ggtccgacga tcctcggatg
     5341 tggggtagcg ccggcccccg tggccggaat ggggtcaacg gacggtaggg tcgctggcgc
     5401 caatccggca ccatcaaccg tcccccggcg ccaagcggcc cagagtcggc ccgcgctacg
     5461 tcttggttcg gcgcgatcgg caggcgcagg tcgcattgcg aagggacgct acaactccgc
     5521 gcccccgcgc cttcccgtct ccgcgcccaa actgctcgcc tcccgactgc tcgcctctat
     5581 tgcaatcgcc cccggccctg gatcggccaa acagcggcga cgatgccgtt gcggacgccg
     5641 atgaagtact cgtgcaggtg gcacatctgg tcgctgtaac ccgactgcag gaagactcga
     5701 tctccgattc gcggacagtc cgacggtgtc gccagcgtga accggccgtg ctcggccgag
     5761 aaactcatgt cgccggtcgt ctcggttcct tccaccgacg gctgacgtgc cgacgggtcg
     5821 atcgacttcc gcccggcgtc gatcatcacc ttttcgggtg tcgggcgact tgtcacctgc
     5881 gccatgatgc tcagggccgg gatcacgttc gcgccgaggt cgcggtacgt gctatcgcca
     5941 aagatgccgc cgccggcctg aacctcggta atgccctcga caccggccga gaccaggtag
     6001 gttccggtgc cgccggctga gacgatctgg acgtcgagtc cggccgcgcg acaggcattg
     6061 gccgtatcga ccagcgaccc gcaggcgcgc cgaatctccg cctcgcgctc ggccgggtcg
     6121 ttgattgcca tcgagtgacc ttcccaggcc atgacgccgg cgaaacgcag gttcggcatc
     6181 gagtcgatca acttggccaa ctcaacggtc ggcccacccg gaagcgttcc gcagcggttc
     6241 atcccggaat tgacctcgat caggatgcgc ggcttcgtcc ccgcctgacc ggcggcgcaa
     6301 tcgagctcgc ggacattgtc cggattatcg accgcgacga ttacgtcggc ttcgcgcgcc
     6361 aaccaggcca accggcgggt cttgatcggg ccgacgacct ggttggcaat cagaatgtcc
     6421 cggatcccgg ccgcggccat cacctcggcc tcgctcacct tggcgcaggt cacgccgatc
     6481 gcgccggcgg caatctcctt gtggacgatg gccggggtct tgttcgcctt gctgtgcggc
     6541 cgccattggg ctccgcgtgc ggcgatatcg gccgccattg tcgcgatgtt gtggtccagg
     6601 gcatccagat cgatgacgag tgccggcgta tcgacgtcgc tcaggggatg gcctacgaga
     6661 gaggtgtaca tcttccggat gcctcgggtt tcactcaaac aaagaagccc cggtgcgctc
     6721 ccgaggcttc gtcatcatac ccggttcgga atcagatcgc ctgaatgtcg tcgatcaacc
     6781 agcgctcaag tcgcgggtcg tagcgcatcg tgaactgctc ggtaccgtcg atcatcgagc
     6841 catcgatgta gcgcacccgg accgtgaccc gcccgtcgtc gaggcgcgtg aagtccgaga
     6901 ttgaatcgat ccgcgtccag ttggaggggt ccttggccgc gacgtcgctg aaggcctccc
     6961 ggaactcatc ctcggacatg cgcgactcgt ccatgaactg gaagaggtag cggtcggtgt
     7021 agagggcgaa cccatgcagc agttcgccga cattcgagca gaagagcagt tcctggatcg
     7081 acgcggtaat tccgtcccgt gtggcgacgt cgaccgggaa tcgggtgatc ctcggctccg
     7141 cgcccgggac gcgcggccgc tcttgatcgt ccggtgtcgg cggcctgcgt ggagtgaagg
     7201 gcacgatctt cccgggctct ccctgcggac caaacagccg cctgagctgc tcctcgagcg
     7261 cctcggcgtc gagaccgcct tgctcggcct ccatccccgg gatcgtacct tgttcgccag
     7321 tttccatggc gccttcctta ctcgcctctt ccccggtcgg tcggccaccc tgcgggatgc
     7381 cggtgccgct cgggcgccgc cgatcgatcc gtccctgctg gacgatctgc cgcagtcgct
     7441 cggccgcaag ctcggcatcg gtctggatcg tcggagcggt cgggaccggg tggatcccgg
     7501 tcggtgtgtc gggcaccccg ggctcccgct cgccgccatc cggcgccggt tgcccacccg
     7561 gcgcgccctc ggtgctcgtc cagggccgga agatcagcgc cagcacgacg attgtcgcca
     7621 gcgtgatcgc gatcaggcca acgagacgcc agccgtccgt tgccgtatca gcatttgcgg
     7681 cttggccgag tggccaccct gcggcgatca tgggcgccgg cccgccggac ggactccgac
     7741 tcgcggtggg agggtagcgg ccgcgcgcgt gcgactgatt tctttcgata ccgactttgc
     7801 cagcgaaagc gccgccaccg ccatggccag catagtactc cagtaccagc tccaactatg
     7861 accatcatgc agtccggata gtgccgctgc cgatgcgctg gcggaatcgg gatcgtcgcc
     7921 ccagggaaag atcggcgacg tgtggaccca gctcgaaata gcctcaatct gatggcggac
     7981 gcgcctgacc tggccgtcga caaccggctg cacgtctggg tcgttcatgc cgctgtcgat
     8041 gagcagtagc accagcatca gaaatgcgag cgtgagaaag aaacgtccca cgtcggggcc
     8101 tttcgggtac ctaccgccgg caatcggcct atatcaaccg cgctcggtgt agtactagtt
     8161 tcctagcccg aaaggtacgt ttcaatggga tgcggaatca atcagtgccg gcaagagagg
     8221 gtcctgttcc cgccaatacc aacgtaccgg caccgggcgg ctgcggcgcc aatgtcgtag
     8281 cgccggtccc cttgtggccg gcatggccat caccacaaaa cggtgtagcg ccggccccat
     8341 tttggccggc tgggtcgacg gtacgcacgg ttgacagagc agacatgctt cctcaaccga
     8401 cccgcccgat atcgaagcga cccagaggct ggccgcgcta cttgtcggta tggtgcttgt
     8461 gacagtgcca tcgggaagcg gaggtcgcat tgccatacga cgctaccaag cgccctccct
     8521 ttccgttccc tccaccctta acctgcccgc ggccggtacc gctgaatcca ctcccggaat
     8581 gccgcgagct ccttcagctg ccgcggcttg gtccagccga gctccttgcc cgcgacttcg
     8641 aggacgcgct catccggccc gatccccgcg tgcggcccgt aggcgatcat ggtccgccgg
     8701 agcaggatgt ccgccagcga caacgccatc tcttcctgga ccgcgaaggc cgcttcggcc
     8761 gcaatcgcgc cggagtagga atcaacgacg gtcgccaacg cgggcgccga cggaatcagc
     8821 gccaggatct cctccgcccg cgtgccgtag agatccacca gccggtaggc cgagcgatcc
     8881 ccgacgccga gttgagcggc tgggcgcacg atgcattgcc ggtaggttcc gaacgccaca
     8941 ccggccgcgc cgggcagggg aaccgaggcg gtccggcttt tggagtcgcg accgagcgcc
     9001 cggagcgcct tgtcgacgaa ctcctcggcc aggctccgat gcgtcgtgag cttgccgccg
     9061 atgaccgacc acaacccccg caggctcggc gcatggtcct tgatcatgtg ccgccgcgtg
     9121 atcgccccgg cgctgccctt cgcggtgcgc ggcaatggcc ggatcccggc gtaggaatag
     9181 cgcacgtcgt ccggcgtcag gttcacgccc cggaagagcg cgtttgcctc gctaagcagg
     9241 taatcgacct cggcgtcgga cgcttcgacc cggtccaaag cgccgtcgta gcggatgtcg
     9301 gtgctgccga gcatgatcat gccgttgaag gggatgacca gaatcgggcg gtgatcggag
     9361 cgcgactcgt agtagatcgc gtcggttggc gcgccctcga atcggtcgac gatgatgtgg
     9421 ctacccttgg tgccgccgat gaaggtgtcg tgaacacgtc ccttcggtgc gccggcgagg
     9481 acctgatcga cccacggacc ggccacgttg accacgaccc gaccggcaat ccggtgctga
     9541 gcgccggtca gggtgtcgcg tacctcgacc ccgcgcacga ccgcgccctc ggagacgatc
     9601 ccggtcacgg tcgcatgggt cacgacctgc gcgccgtgct cgacggcgga gagtacgttc
     9661 tcgaccacca gccgctcggc gaaggtcacc
//
LOCUS       DATAOZ010000355         6124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1988944, whole genome shotgun sequence.
ACCESSION   DATAOZ010000355 DATAOZ010000000
VERSION     DATAOZ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988944"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /gene="nusA"
                     /locus_tag="VHR64_09070"
     CDS             <1..845
                     /gene="nusA"
                     /locus_tag="VHR64_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX3303018.1"
                     /translation="GKAEAVMPAREQVPTERYRPGPRIKVLLLEVNKDPKGPQLIVSR
                     SHPNLIRRLFEIEVPEIYSGAVEIMAIAREPGLRSKVAVAARQEKVDPVGSCVGVRGV
                     RIQNIVNELYGEKIDVIEWSPDTATFIANALSPAKPTNVSLSEAENIATVIVPSDQMS
                     LAIGKEGQNARLAYKLTNWRIDIKDPESLKDEDLEMLRQARADYQQEAAGGLVWQGRQ
                     PRLVRADGMVAVREQEYGPLPEDLIGMSVDVDMVGEVLEVYYNRALRARFNVASGEQL
                     PLDE"
     gene            865..1215
                     /locus_tag="VHR64_09075"
     CDS             865..1215
                     /locus_tag="VHR64_09075"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX3303019.1"
                     /translation="MVKQTSAPESKGKSRKRPRPKHVPIRTCAICREKDTKRGLTRVV
                     RQPDGTVVIDPSGRLNGRGAYICDKQSCWDRAASTDVLAKALNVELSQEFRDELRQFG
                     RAVGDNTATDPTEM"
     gene            1324..3129
                     /gene="infB"
                     /locus_tag="VHR64_09080"
     CDS             1324..3129
                     /gene="infB"
                     /locus_tag="VHR64_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX3303020.1"
                     /translation="MRRTAPVVREPLSLPPVMTVAELAEKTQSTPIEVIKELMKIGVM
                     ANINQQIDYDTAHRVATALGWETNEDIPEVVQRANADFESRLLEAMSDPSATTRPPVV
                     TIMGHVDHGKTKLLDAIRQTKVAEGEAGGITQHIGAYQVETQGRKVTFLDTPGHEAFT
                     AMRARGAQVTDVAVLVVAADDGVMPTTREAVAHIKSASVPMIVAINKIDLPAANPDRV
                     KQQLAEIEVVPEEWGGDVPFVEVSAREKLGLDDLLDVILLVSDVHELKANPNRLANGT
                     VIEAKVDRNRGPVATILIQSGTLNMRDIVVAGATWGRVKAMQDDHGRRVRKAGPSTPV
                     EILGLLDVPQAGDQVQVFEDEREARELVESRVQQRRIEQLTADRAMKLTDIASQVSSG
                     ATATAELRVILKADVQGSLGAIQAALLKLNDETDAGVRLSITYAGTGAISESDVSLAV
                     ATKSVIVGFNVRPDVAAKRAADASGVDIRFYNIIYNLIDEVKQAMTGLLAPVFQDVTD
                     GYAEVRETFKLPSGDVVAGCYVLDGKLVRNSRVRVLRSGTVMFEGGIKSLRRFKDDVR
                     EVASGYECGLGLEGFNDLQVKDQVECFHRQEVARS"
     gene            3159..3539
                     /gene="rbfA"
                     /locus_tag="VHR64_09085"
     CDS             3159..3539
                     /gene="rbfA"
                     /locus_tag="VHR64_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX3303021.1"
                     /translation="MTRRTRQVGELLKEELTDIIRKEMDDPRLAFWSITRVDVPTDLR
                     SARVYVSVLGTDEERAATLSAMRSGAGFIRRHLKPRLHMRQIPELEFRDDRSLEHAAA
                     ISKTLHDLGASGSAADDRTETSDH"
     gene            3511..4515
                     /locus_tag="VHR64_09090"
     CDS             3511..4515
                     /locus_tag="VHR64_09090"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX3303022.1"
                     /translation="MTEPKHQIIDPAAADAALSMLSAARRILVPTHQNVDADGLASPL
                     ALMHALKPQGIEIVPVVSDGKLPKSLSFLPGVKSALLYGHDDLPDFDLICMLDCSDRR
                     RLAGFYQDDPGRIEGNYPIVNIDHHVTNDHYGVVNIVVPQAAATAEIVAELIKYWGIR
                     LTKPIAQCLLAGIYGDTLGLRTDSTSPWTMRTSAELVDAGANPAKITDALFRLKPKST
                     VCLWEHALRKVAWEGKLIWTEVDHRDLSACNADPSEAEGIVNFLLGTDGSRAAAMLYQ
                     TDSGYRVSMRSLSDEVDVAAIASVFGGGGHPRAAGCTVTGGLHERDEFLSKVAELLEH
                     "
     gene            4532..5425
                     /gene="truB"
                     /locus_tag="VHR64_09095"
     CDS             4532..5425
                     /gene="truB"
                     /locus_tag="VHR64_09095"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX3303023.1"
                     /translation="MTPALHGILMIDKPAGWTSHDVVGWVRRWARERKVGHAGTLDPA
                     ATGVLPVAINDGTRVLEFLSDASKAYRAEITFGVETDSADGDGTVVAISDATFTETDL
                     KRALEAFRGPITQRPPAHSAIKIGGKRAYELARAGTDVDIPERAVTIFSLDVVEWDGV
                     SLTVDLDCSKGTYIRSIARDLGAALGCGAYLSNLVRTRSGPFNLCDAWTIDELEAIDP
                     REQWSSIAEHPDSALVDWPAIILGEIASEDWRHGRPVPVRESAGQSRARVYDNSGHWR
                     GLAEHDRAADVWKALRVINPQ"
     gene            5422..>6124
                     /locus_tag="VHR64_09100"
     CDS             5422..>6124
                     /locus_tag="VHR64_09100"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX3303024.1"
                     /translation="MSTVLVNGLRDVQIPASVVTIGTFDGVHVGHQYLLRRAVERGEE
                     LGLPVVVITFEPIPASVLRPDGFAGRICAAEEKRSLITEHCADVLVTMTFDLELAQWS
                     PEQFMDAVVAATGVKELWIGEAFALGKGRAGGVEQLTEIGLAQGYAVCALERREDIDG
                     VISSSRIRHAIQLGDVSLANRLLGRPFTVTGEVIHGAQFGRTIGFPTANVVPPFEQVA
                     LADGIYASRALLPGEK"
BASE COUNT         1179 a         1843 c         2036 g         1066 t
ORIGIN      
        1 tcggcaaggc cgaggcggtg atgcccgcgc gcgagcaagt gccgactgag cggtatcggc
       61 cgggaccgcg gatcaaggtc ttgctgctcg aggtcaataa ggacccgaaa ggtccgcagc
      121 tgatcgtttc ccggtcgcac ccgaacctga ttcgacgcct cttcgagatc gaagtgccgg
      181 agatctacag cggtgcggtg gagatcatgg cgatcgcgcg ggagcccggt ctgcgctcca
      241 aggtcgcagt cgccgcccgg caggagaagg tagatccggt cggctcttgc gttggcgttc
      301 gcggtgtccg tatccagaac atcgtcaacg agctctatgg cgaaaagatc gacgtgatcg
      361 agtggtcgcc ggacacggcg actttcattg ccaatgcgct cagcccggcc aagccgacca
      421 atgtctcgct ctccgaggct gagaatattg cgacggtcat cgtgccgtcc gaccagatgt
      481 cgctcgcgat cggcaaggag ggccagaacg cccgtttggc ttacaagctg accaactggc
      541 ggatcgatat caaggatccc gagtccctca aggatgagga tcttgagatg ctgcgacagg
      601 cccgtgcaga ctaccagcag gaagcggccg gtggcctcgt ctggcaggga cggcagccac
      661 ggctggtccg ggccgacggt atggttgccg tccgggagca ggaatatggt ccgctccccg
      721 aggacctcat cggcatgagt gtcgacgtgg atatggtggg cgaggtgctc gaggtctatt
      781 acaaccgggc attgcgcgcc cgcttcaacg tagccagcgg cgagcaattg ccactggatg
      841 agtagcccgg ggagtcaggc ggtgatggtg aaacagacca gcgcgccgga atcgaagggc
      901 aagtcgcgca aaagaccgcg gccgaagcat gttcccatcc gaacgtgcgc catttgccgg
      961 gagaaggaca caaagcgcgg gctgacccgc gtcgtccgtc aaccggacgg aaccgtggtc
     1021 atcgatccga gcggccggct caacggccgc ggcgcgtata tatgtgacaa gcaaagctgc
     1081 tgggaccgag cagcgtccac cgatgtgctg gcgaaggcgc tcaatgtgga gttgtcgcag
     1141 gaatttcgcg acgagcttcg ccaatttggt cgggccgtgg gagacaacac cgcaaccgac
     1201 ccgacagaaa tgtgaggaac ctatatggca caggaattta gcaacggcgg tccacgcggt
     1261 ggccgacgat caggcgggaa gcggaataac cgcggacgct ccggtggcgc tcgcacagtc
     1321 acgctgcgac gtaccgctcc ggttgtccgc gagccgcttt ctctgccccc ggtaatgaca
     1381 gtcgctgaac ttgccgagaa aacgcaatca acgccgatcg aggtcatcaa ggagctgatg
     1441 aagatcggtg tgatggccaa tatcaaccaa cagatcgact acgacacagc tcaccgggtc
     1501 gcgaccgcgc tcgggtggga gacgaatgag gatattcccg aggtcgtcca gcgggcgaac
     1561 gcagacttcg agagccgtct gctcgaggcg atgagcgatc cgagcgcgac cactcgaccg
     1621 ccggttgtga ccatcatggg tcacgtcgac cacggcaaga ccaaactgct cgacgctatt
     1681 cggcagacga aggttgccga gggtgaggcc ggcggcatta cccagcacat cggcgcgtac
     1741 caggtcgaga cccagggtcg gaaggtcact ttcctcgata ccccgggtca cgaggcgttt
     1801 accgccatgc gtgcccgcgg cgcccaggtc accgacgtgg ccgtgttggt cgttgcggcg
     1861 gatgacggcg tgatgccgac aacgcgcgag gccgttgccc acatcaagtc ggccagcgtg
     1921 ccgatgatcg tggcaatcaa caagatcgac ctgcccgctg cgaacccgga tcgggtcaag
     1981 cagcagctcg ccgagatcga ggtcgtccct gaggaatggg gtggcgacgt cccgtttgtc
     2041 gaagtctcgg cgcgcgagaa gctgggcctg gacgatctgc tcgacgtgat cctgctcgtc
     2101 tccgacgtgc atgagttgaa ggcgaacccg aaccggcttg ccaacggcac ggtgatcgag
     2161 gcgaaggtcg accgcaaccg aggcccggtc gcgacgatcc tgatccaaag cggcacactc
     2221 aacatgcgcg atatcgtcgt cgccggtgcg acctggggcc gggtcaaggc gatgcaggac
     2281 gatcacggcc gccgcgtgcg caaggctgga ccgagcacgc cggtcgagat ccttggtctg
     2341 ctcgacgtgc cacaagccgg cgaccaggtc caggtattcg aagatgagcg cgaggcccgc
     2401 gagctggtcg agtctcgcgt gcagcagcgc cggatcgagc agctcactgc cgaccgggcc
     2461 atgaagctga cagacatcgc ctcgcaggtc agcagcggtg ccacggccac ggccgagctg
     2521 cgcgtgatcc tgaaggcgga tgtgcagggg tcgctcggcg cgatccaggc cgcattgctc
     2581 aaactgaatg atgagacgga tgcgggtgtc cggttgagca tcacgtacgc cggtacgggc
     2641 gcaatctccg agtccgatgt cagcctcgcg gtcgcgacca agagcgtcat cgtcggcttc
     2701 aacgtccggc cggacgttgc ggccaagcgg gcggccgatg ccagcggcgt cgacattcgc
     2761 ttctacaaca tcatctacaa cctgatcgac gaagtgaagc aggcgatgac gggtctgctc
     2821 gcgccggtct tccaggacgt caccgacggc tacgcggagg tccgcgagac attcaagctg
     2881 ccgagcggcg atgtcgtggc cggctgctac gtcctggacg gcaaactggt ccgcaactcg
     2941 cgggtgcgcg tgctgcgcag cggtacggtc atgttcgagg gcggcatcaa gtcgctccgc
     3001 cgcttcaagg acgacgtgcg cgaagtcgcg tccggctacg aatgcggact tggcctcgag
     3061 ggattcaacg atctccaggt caaggaccag gttgagtgct tccaccgcca ggaggtcgct
     3121 cgttcctagg gcatgcgacg ggaaggggag gcgcgatcat gacacgacga acacgacaag
     3181 tgggtgagct gctcaaggag gagctcaccg acatcattcg caaggaaatg gacgatccgc
     3241 gcctggcgtt ctggagcatc acccgcgtcg atgtgccgac ggatctccgc tctgcgcgcg
     3301 tctatgtgag cgtattgggg acggacgagg agcgcgcggc gacgctttcg gcaatgcgct
     3361 ccggagccgg cttcatccgt cggcacctca agccgcgcct acacatgcgg caaatccccg
     3421 aactagaatt tcgggacgat cgctctctgg aacacgcggc ggcgatctcc aagaccttgc
     3481 acgaccttgg agcgagtgga tctgcggctg atgaccgaac cgaaacatca gatcattgat
     3541 cccgccgccg cggatgccgc gctcagcatg ctcagcgcgg cgcggcgaat cctggtcccg
     3601 acccaccaga acgtcgatgc cgatggtttg gcatcaccgc tcgccctgat gcacgcgctc
     3661 aagccccagg gtattgagat cgtgccggtc gttagcgacg gcaagttgcc gaagtcgctc
     3721 tcctttctgc ccggcgtcaa gagtgccttg ctgtacgggc acgacgacct cccggacttc
     3781 gacctcatct gcatgctgga ttgctcggat cgacgaaggc tggcggggtt ctatcaggac
     3841 gatccgggcc ggatcgaggg caactatccg atcgtcaaca tcgatcatca cgtgaccaac
     3901 gaccactacg gagtggtcaa catcgttgtg ccgcaggctg ctgccacggc ggagatcgtc
     3961 gccgaattga tcaagtactg gggaatccgc ctcaccaagc cgatcgccca gtgcctgctc
     4021 gccggaatat atggcgatac gctgggcttg cggacagatt cgaccagtcc gtggacgatg
     4081 cgcacgtcgg ccgagttggt cgatgccggc gccaatccgg ccaagatcac cgacgcactc
     4141 ttcagactga agccaaagtc gaccgtctgc ctgtgggagc acgccctgcg caaggttgcc
     4201 tgggaaggca agttgatctg gaccgaggtc gatcaccgcg atctttcggc ctgcaatgcg
     4261 gacccatccg aagcagaagg catcgtcaat ttcctgctcg gtaccgacgg ctcgcgggcg
     4321 gcggcgatgc tttaccagac cgattccggc taccgggtca gcatgcgcag tctctcggac
     4381 gaagtcgatg tcgcggcaat cgcctcggtg ttcggcggtg gtgggcatcc ccgggcagcc
     4441 ggttgcaccg taacgggcgg ccttcacgag cgcgacgagt tcctcagcaa ggtcgccgag
     4501 cttctagagc attgacccgg ggtcgaccgg aatgactccc gcactccacg gcatcctgat
     4561 gatcgacaag ccagcaggtt ggaccagtca cgatgtggtt ggttgggttc gcaggtgggc
     4621 ccgggagcga aaggtcgggc atgccggaac gcttgatccg gcggcaaccg gtgtcttgcc
     4681 agtcgcgatc aacgacggca cgcgcgtgct ggagttcctg agtgacgcga gcaaggccta
     4741 tcgtgcggag attacgtttg gggtcgagac cgacagtgcc gacggcgacg gcaccgtcgt
     4801 cgcgatcagc gacgccacct tcacggaaac cgatctcaaa cgcgccctcg aggcgttccg
     4861 tggaccgatc acgcagcggc caccggctca ctctgccatc aagattggcg gcaagcgcgc
     4921 ctacgaactg gcccgggcgg ggacggacgt cgacatcccg gagagagcgg taacgatctt
     4981 ctcgctggat gtcgtcgagt gggatggtgt gtcgctgacc gtcgacctcg attgctcgaa
     5041 ggggacgtat attcgttcga tcgcgcgcga tctgggtgcg gcgctcgggt gcggcgcgta
     5101 tctttcgaat ctggtgcgga ctcgaagcgg cccgttcaac ctttgcgatg cgtggacgat
     5161 cgatgagctc gaggcgatcg atccccggga gcagtggtcg tcgatcgctg agcatcccga
     5221 ttcggcgctg gtcgattggc cggccatcat tttgggcgag atcgcgtcgg aagactggcg
     5281 ccatggacgc cctgtgcctg tgcgcgaatc agccgggcag tcgagggctc gcgtgtatga
     5341 caattcgggc cattggcgcg gcctggctga gcatgatcgg gccgcggacg tttggaaagc
     5401 attgagagtg atcaacccac agtgagcacg gtactcgtca acggactccg tgacgttcag
     5461 atcccggcat cggtcgtaac gatcggcacc tttgatggcg tgcacgttgg ccatcagtac
     5521 ctgttaaggc gcgcggtcga gcggggcgag gagctcggac tgccggtcgt cgttatcacg
     5581 ttcgaaccga taccggcgag cgtgcttcgg ccggacggtt ttgccggtcg gatctgcgcg
     5641 gcggaggaga agcgttcgct gatcacggaa cactgcgccg atgtgctggt gaccatgacg
     5701 ttcgatctcg agctggcgca atggtcgccg gagcaattca tggacgcggt cgttgccgca
     5761 accggcgtca aggagctttg gattggcgag gcattcgcgc tcggaaaggg tcgcgccggc
     5821 ggcgtcgaac aacttacgga gatcggcctg gcacagggtt atgccgtttg tgcacttgag
     5881 cgacgcgaag atatcgacgg cgtgattagc agcagccgga tccgccacgc gattcaactc
     5941 ggcgatgtct cgctggccaa tcggttgctc ggccggccat tcacggtcac cggcgaggta
     6001 atccacggcg cgcaattcgg ccgcacgatt ggcttcccga cagcgaacgt cgtaccgcca
     6061 ttcgagcaag tggcgttggc cgacggtatc tatgcgtcgc gcgccctcct tccgggagag
     6121 aaaa
//
LOCUS       DATAOZ010000356         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7513937, whole genome shotgun sequence.
ACCESSION   DATAOZ010000356 DATAOZ010000000
VERSION     DATAOZ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7513937"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VHR64_09105"
     CDS             complement(<1..289)
                     /locus_tag="VHR64_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303025.1"
                     /translation="MAKRGAFTFVLHSHLPYARNAGMWPHGEEWIHEAIADTYIPILD
                     ALNELVDEGVDFKLTIGITPILAEQLADESIINNFIQYAAERAAWASNDVTR"
     gene            complement(341..1924)
                     /gene="malQ"
                     /locus_tag="VHR64_09110"
     CDS             complement(341..1924)
                     /gene="malQ"
                     /locus_tag="VHR64_09110"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX3303026.1"
                     /translation="MGPETLRRGSGILLHPTSLPGPDGIGDLGDGAYRFVDWLAQAQQ
                     RYWQVMPLVPTGYGDSPYAAPSAFAGNPLLISLDILIGDGLLDPHAFRNTPFNENVVN
                     YGGARTQKERALRAAFQRFEEREPGDLDREFKAFRKAEAWWLDDFCLFIALKDEHGDC
                     PWSDWPDEIKRRKPSAMKAARERLSAEIRYHAFCQWLFRRQWYALRVYANERNVRIIG
                     DIPIFVAFDSADVWSHPGLFRLDEQLNPVVVAGVPPDYFSLDGQRWGNPLYDWDAMSA
                     TGYAWWISRFRALLALVDVVRIDHFRGFAANWSVPASAPTAAEGRWERGPGRELFDAV
                     VGKLGTVPIIVEDLGLITPDVVALREELGLPGMAVLQFAFDGDPHNAYLPHSLERNSV
                     VYTGTHDNQTTVGWFQSMPEHVRTEVLAYLGRDGSDIAWDFIRAALNSPAIMAIIPMQ
                     DVLRRGDDARMNTPGRPDGNWTWRMRWTDLNAGLAEGLGLLTHLSGRATAEKQPSGAD
                     PFDYTEPGTAHPLHDPAVI"
     gene            complement(1933..>2559)
                     /locus_tag="VHR64_09115"
     CDS             complement(1933..>2559)
                     /locus_tag="VHR64_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX3303027.1"
                     /translation="YLISEPLAAAIGAQIPVADPSGNMVIDIGGGTTEVAVISLNGIV
                     VARSLRVGGNKFDEAISNYIKRKYNLRVGDRTSEDIKIQIGSAMPLEEDEWMEVRGRD
                     EVAGLPRTIQIHSGEVTDAITEPLEAIIGAVRAVLEDTPPELSSDIIDKGMILTGGGS
                     MLRHLPDLLTEVTGVPCYVADDPLSCVAVGTGLALEHMDILRDSLEEP"
BASE COUNT          484 a          793 c          813 g          469 t
ORIGIN      
        1 agcgcgtgac gtcgttcgag gcccaggcgg ctcgctccgc cgcatactgg atgaaattat
       61 tgatgatcga ttcgtcggca agctgttcgg cgaggatcgg ggtgatcccg atcgtcagct
      121 tgaaatcgac gccctcgtcg acgagctcat tgagcgcgtc gagaatcggg atgtaggtgt
      181 cggcgatggc ctcgtggatc cattcttcgc cgtgcggcca catgcccgcg tttcgcgcgt
      241 acggcaggtg actgtggagg acaaaggtaa aggctccgcg ttttgccatt cagcaggtgt
      301 cctttcaacg cgtgtcggat ccgaggccct gccggcaagt ctaaatgacg gcgggatcgt
      361 gcagtggatg tgcggtacct ggctctgtgt aatcgaatgg atcggctccc gacggctgct
      421 tttcggccgt cgcgcgaccg gagaggtgcg taagcagacc gaggccttca gccagacccg
      481 cgttgaggtc cgtccagcgc atgcgccagg tccaattccc atccggacga ccgggcgtat
      541 tcatgcgagc gtcatcgcca cggcgcagga cgtcctgcat cgggatgatg gccatgatcg
      601 caggcgagtt cagggcggcg cggatgaaat cccacgcgat gtcgctgcca tcacgaccca
      661 aataggcgag gacctcggtg cggacgtgct ccggcatgga ctgaaaccag cctaccgtcg
      721 tctggttgtc atgcgttccc gtatagacga ctgaattgcg ctcgaggctg tgcggcaaat
      781 aggcgttgtg gggatcgccg tcgaaggcga actgcagcac tgccataccc ggcagtccaa
      841 gctcctcgcg gagggcgacg acgtccggcg tgatcaagcc gaggtcctcg acgatgatcg
      901 gaaccgttcc gagcttcccg acgaccgcat cgaacaactc gcgacccggt ccgcgctccc
      961 accgtccctc agcggccgtg ggcgcggacg cggggacaga ccagttggcc gcaaatccgc
     1021 ggaaatggtc gatccggacg acgtcgacga gggcgaggag cgcccggaac cggctgatcc
     1081 accacgcgta gccagtggcc gacatggcat cccagtcgta gagcgggttt ccccagcgct
     1141 gtccatcgag tgaaaagtaa tccggcggca cgccagcgac gaccaccgga ttcaattgct
     1201 catcgaggcg aaacaggcct gggtgagacc agacatcggc gctgtcgaac gccacaaaga
     1261 tcgggatgtc gccgatgatc cgcacgttgc gctcattcgc atacacgcgc agtgcatacc
     1321 actgccggcg gaaaagccac tgacaaaacg cgtggtagcg aatctcggct gaaaggcgct
     1381 ccctggcggc tttcatggcg gaaggtttgc gccgcttgat ctcgtccggc caatcggacc
     1441 agggacagtc gccgtgctca tccttgagcg cgatgaacag gcaaaagtcg tccagccacc
     1501 atgcttccgc tttgcggaag gccttgaact cgcgatcgag gtcgccgggt tcccgctcct
     1561 cgaatcgctg gaatgctgcc cggagcgccc gctccttctg ggtgcgcgca ccgccgtaat
     1621 tgacgacgtt ctcgttgaag ggcgtgtttc gaaaggcgtg cggatcgagt agcccgtcgc
     1681 cgatcaggat gtcgagcgag atcagcaggg ggttgccagc aaacgcagaa ggtgccgcat
     1741 acgggctgtc gccatatccg gtcgggacca gcggcatgac ctgccaatac cgctgctggg
     1801 cctgtgcgag ccaatcgaca aatcgatatg ccccgtcgcc gagatcgccg atcccgtcag
     1861 gtcctggcag cgaggtcggg tgaagaagaa tgccgctgcc ccggcgcaga gtttccggtc
     1921 ccatcacgtg cgctatggct cctccagact atcgcggaga atatccatgt gctcgagcgc
     1981 tagtccggtt ccgaccgcca cacaagagag cggatcatca gccacgtagc acggcacgcc
     2041 ggtaacctcg gtcagcaggt cgggcaaatg gcgcagcatc gagccgccgc cggtcaagat
     2101 cattcccttg tctatgatgt ccgacgacaa ctcgggaggc gtgtcctcga gtaccgcccg
     2161 cacagcgccg atgatcgcct cgagtggctc ggtgatcgcg tcggtcactt cgcccgagtg
     2221 aatctggatc gtgcgcggca ggccggcgac ctcatcgcgg ccgcggacct ccatccactc
     2281 gtcttcttcg agcggcatgg cgctgccgat ctggatcttg atgtcctcgg acgtgcgatc
     2341 gccgacgcgc aggttgtact tgcgcttgat gtaattgctg atcgcctcgt cgaatttgtt
     2401 gccgccgacc cggagtgacc gcgcgacgac gatgccattc agcgagatga ccgcaacttc
     2461 agtcgttccg ccaccgatgt cgatcaccat gttgccagag ggatcggcga ccggaatctg
     2521 ggcgccgatt gcggcggcca aaggctcgct gatcagata
//
LOCUS       DATAOZ010000357         5266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1727649, whole genome shotgun sequence.
ACCESSION   DATAOZ010000357 DATAOZ010000000
VERSION     DATAOZ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727649"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VHR64_09120"
     CDS             complement(<1..192)
                     /locus_tag="VHR64_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303028.1"
                     /translation="MISSRIFVTCLLWAMICGGLLAILDSPIAGLVLGAVLAIGAAAL
                     LPCSCFVDWDAKLAMRAHDS"
     gene            complement(189..2282)
                     /locus_tag="VHR64_09125"
     CDS             complement(189..2282)
                     /locus_tag="VHR64_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX3303029.1"
                     /translation="MTIGAPTETSPAERESSGLPEDQDHLTVIHGRLPGDIYVRRPTH
                     LPEPEHDGHEDGYELRPDDGPGVASANVVGRVRRKLIGTPIPSDQEHVERLGVATGLP
                     VFASDNISSSAYATEEIMRVLVLAGVGALWLTLPITFGIVAVLAIVVISYRQTIAAYP
                     SGGGSYIVARDNLGDKAALTAAGALLVDYVLTVAVSIAAGVAALTSLFPALYPHRVLA
                     GVVFVLLIMLGNLRGIRESGAIFAAPAYVYLASMFGLLGYGLFRYLSGTMPHYTAPAG
                     WAAAAGTQGLGLLLILRAFASGSVALTGTEAVSNGVPAFKAPEPRRAQTVLVLMGSSF
                     ATIFIGISFLAGHLGILPDPSEEQTIISQLTRTLVGNGPYLYLVQLATALLLVLAANT
                     AFADFPRLLSILARDRFVPRLFSFRGDRLAFTSGIVLLSVLSAILIVVFSGSVTQLIP
                     LYTVGVFVAFTLSQSGMVKHWWKLRGTERKWRRRAVINGIGAVTTGIVAIEVGISKFL
                     LGAWMVLILVPLLIAGMWGIHRHYRRLASAQLPETPLDYTRLRIRPIVPIADLNVPAK
                     QALAFARAVADDDTITLVHVTDKIDDANRLRAEWDQWPHGATHFVVVESPYRSLGGPL
                     VRFLRDEAREHPGATLLVVLPEYVPGRWWEHLLHNQTALRLKAKLLFQPGVFVVSVPY
                     HIAHASEPGSMGARP"
     gene            complement(2418..2876)
                     /locus_tag="VHR64_09130"
     CDS             complement(2418..2876)
                     /locus_tag="VHR64_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303030.1"
                     /translation="MSDTQELVYTDTEINQQALANASAMPYLMVAFAQATGRTPEEAA
                     EFVGNIFAPGWSRFTGMGALFIVRQMALSIVCCGGEVQELTGDEQSAEAWVTGVPVQE
                     EADFFGISCDEADRFCNVFAPIATSLGFDFEWRRDDETLILSVRRGDAEV"
     gene            3234..4409
                     /locus_tag="VHR64_09135"
     CDS             3234..4409
                     /locus_tag="VHR64_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX3303031.1"
                     /translation="MLGQNLKYGRLTRRQALVVGGGALAGAAIAGPILAPERYHAGAS
                     NGGQTREYFIAAEEVDWDYAPSGKDLITGEPFSEAAQVFTEPGPNRIGHVYRKALYHA
                     FTDDTFATRAAADPRWEHLGILGPVIHAEVGDTIVVHFKNNTTRPTTMHPHGVFYDKD
                     SEGAGYADGSGDSDHADDAVESGATYTYTWKVPERAGPAMHDPSSIVWMYHSHVDEPA
                     DTYAGLSGPIIITRAGMAKPDGSPTDVDREFVLLFEVMDENASRYITDNVAYHIHPEG
                     DSDEHQDFDDEFIESNLMHGINGYVYGNLPGLTMRVGERVRWYVMGMGTEVDLHTPHW
                     HGNTLTMMGMRTDMVELLPMSMKTLDMTPDAVGTWLLHCHVNDHIVAGMQALYTVTE"
     gene            4533..4751
                     /locus_tag="VHR64_09140"
     CDS             4533..4751
                     /locus_tag="VHR64_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303032.1"
                     /translation="MAEEPFSYRRTKAGDVFISWNGKSVTTLKGKAAATFLAKIADLD
                     DEKAQLLMAKATGHFKHGNERPSGPDRV"
     gene            4855..5058
                     /locus_tag="VHR64_09145"
     CDS             4855..5058
                     /locus_tag="VHR64_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303033.1"
                     /translation="MTHETAEGSNPEPFEPVLLKWEPWRPQEVAHLLAQVEAPWYVAA
                     GWAIDLFLGCPRRAHEDIEIGVP"
BASE COUNT         1032 a         1697 c         1698 g          839 t
ORIGIN      
        1 ggagtcgtgc gcgcgcatcg ccagcttggc gtcccagtcc acgaagcagc tgcagggcag
       61 caaggccgcg gccccgatcg ccagcaccgc gccaagcacc aacccagcga tcgggctgtc
      121 caggatggca aggagtccgc cacagatcat ggcccagagc aggcacgtca cgaagatccg
      181 cgaggagatc atggtctcgc tcccatgctg cccggctcgc tggcgtgcgc gatatgatac
      241 gggacgctga cgacgaacac tcccggctgg aagagcagct tggccttgag ccggagtgcg
      301 gtctggttat gcagcaggtg ttcccaccat cgcccgggca cgtattccgg cagaaccacg
      361 agcaaggtcg cgccgggatg ctcgcgcgcc tcgtcgcgca ggaagcggac aagcggtcca
      421 ccgagactgc gatagggcga ttcgaccacc acgaaatgcg tcgcaccatg cggccactgg
      481 tcccactcgg cacggaggcg attggcgtcg tcgatcttgt cggtgacgtg cacgagcgtg
      541 atcgtatcgt cgtcggccac ggcccgggca aaggcgagcg cttgcttggc cggcacgttc
      601 agatcggcaa tcggcacgat cggccgaatc cgcagccgcg tgtaatccag cggagtctcg
      661 ggaagctgcg cgctggccaa tcgtcggtaa tggcggtgga tgccccacat cccggcgatc
      721 agcaagggca cgaggatgag caccatccag gcgccgagca ggaacttgga gatgccgact
      781 tcgatcgcga cgataccggt cgtcaccgcg ccaatgccgt tgatgaccgc gcgccgtcgc
      841 cacttccgct ccgtcccgcg tagtttccac cagtgcttga ccatcccgct ctggctgagg
      901 gtgaaggcca cgaagacgcc gaccgtgtag agcggaatca gctgggtgac gctgcccgag
      961 aagaccacga tcaggatcgc cgacaagacg gaaagcagca cgatcccgct cgtgaaggcc
     1021 aatcgatcgc cccggaaact gaagagtcgg ggaacgaacc ggtcgcgcgc caggatgctc
     1081 agcaagcgcg ggaagtcggc gaaggccgtg ttggcggcca gcaccagcag gagtgccgtg
     1141 gcaagttgga ccaggtagag gtacggcccg ttgccgacca gcgtccgtgt gagctggctg
     1201 atgatggtct gctcctcgct cggatcgggc aggatgccaa ggtgaccagc caggaagctg
     1261 atcccgatga agattgtggc gaacgagctg cccattagca ccagcacggt ctgcgcccgg
     1321 cgcggctccg gcgccttgaa cgccggcact ccattcgaga ccgcctcggt cccggtcagc
     1381 gcgaccgatc ctgaggcgaa cgcacgcagg atcagcagca atcccagccc ctgtgtcccg
     1441 gccgctgccg cccaaccggc cggtgccgtg tagtgcggca tcgtgccgga taagtaccgg
     1501 aaaagcccat atccgagcag gccgaacatg gaggcgaggt agacataggc cggcgcggcg
     1561 aagatcgctc cgctttcccg gatgccccgg agattgccga gcatgatcag caagacgaag
     1621 acgaccccgg ccagcacccg atgcgggtag agcgcgggga agagcgatgt cagcgccgca
     1681 accccggccg cgatcgaaac cgcgaccgtc aggacataat cgaccagcag cgcgcccgcc
     1741 gcggtcagcg cagccttgtc cccaaggttg tccctggcca cgatgtacga gccgccgccc
     1801 gaggggtagg ccgcgatcgt ctgccggtaa ctgatgacca cgatcgcgag gacggcgacg
     1861 atcccgaacg tgatcggcag cgtcagccag agcgcgccga ccccggccag aaccagcacg
     1921 cgcatgatct cctcggtcgc gtaggccgat gaggagatgt tgtcagacgc aaagaccggc
     1981 aggccggtcg ccacgccgag ccgctcgacg tgctcctgat cgctcgggat cggggtgccg
     2041 atcagcttcc gccggaccct gccgaccacg tttgcggatg cgacacccgg cccgtcgtcc
     2101 ggccggagct cgtagccgtc ctcatgtccg tcgtgctccg gttccggcaa atgggtcggc
     2161 cggcgaacat agatgtctcc cggcaaccgg ccgtggatca cggtgaggtg atcctggtcc
     2221 tcggggagac ccgagctctc ccgttcggca ggcgatgttt cggtaggcgc tccgatggtc
     2281 acgagtcgcg cgctcctgcg atccggcgac cgtcaatcgg tcgccatcaa acgtcaggag
     2341 cctacgcctg tgcgcattaa tgcggagtta cgggaagccc aaaacccata cgaatcctgt
     2401 tagaaagatt cgcgtcgtca cacttccgcg tcgccgcgcc gcacggagag gatgagcgtc
     2461 tcgtcgtccc gccgccattc gaagtcgaat ccgagcgacg tggcgatcgg agcaaaaacg
     2521 ttgcagaagc ggtccgcctc gtcgcagcta atgccgaaga agtcggcctc ctcctgtacc
     2581 ggcacgccgg tgacccaggc ctccgccgac tgctcgtctc cggtcaattc ctgcacttcg
     2641 ccgccgcagc agacgatgct gagcgccatc tggcgaacga tgaagagggc gcccatcccg
     2701 gtaaagcgcg accagcccgg cgcaaagatg ttcccgacga attccgcggc ctcctcaggt
     2761 gtgcgcccgg ttgcttgagc gaacgcgacc atcaggtacg gcattgcgga ggcgttggcc
     2821 aacgcctgct gattgatctc ggtgtcggtg tacaccaatt cctgggtgtc ggacacggga
     2881 catccttctg cgccaactcg agttcacgcc tctgcgccaa ttctggccga ttcgtttgtg
     2941 ctgtcaaggt gactctcggc atgggttgac cgttccgttc gccgcgatgc gcgagtactt
     3001 ccccgtgtgc ggcgaacaga ccaacgagcc aggtgcagga tcgtacagcg gcgtgccggg
     3061 cgcttctact aatgacacgc gctgtacagg aaggcgaggc tccggcgtca agagccaagc
     3121 gtacaaacat cttgactcga cccgccacgg ggcctagcat cggcacagtt ccaacaatcg
     3181 agtggctggg tttgtccaac ggtgcgttcg cgtctcctcg ggaggaggtc gtcatgcttg
     3241 ggcagaacct gaagtacggt cgtctcacgc gccgccaggc gctcgtcgtc ggcgggggcg
     3301 ccctggccgg cgccgccatc gccgggccaa tcctcgcgcc ggagcgatat cacgccggag
     3361 cgtcgaacgg tgggcaaacg cgggagtatt tcatcgccgc ggaggaggtt gattgggatt
     3421 acgcgccctc cggcaaggac ctcatcacgg gggaaccgtt tagcgaagca gcgcaggtct
     3481 ttaccgagcc gggaccgaac cggatcggtc acgtctaccg gaaggccctg taccatgcgt
     3541 tcacggacga cacgttcgcg acgcgggccg ccgctgaccc acgctgggag cacctcggca
     3601 tcctcgggcc agtgatccac gccgaggtcg gcgacacgat cgtcgtccac ttcaagaaca
     3661 acacaaccag accgacaacc atgcacccgc acggtgtctt ctacgacaag gacagcgaag
     3721 gcgcaggcta tgcggacggg agtggtgaca gcgatcatgc tgacgatgcg gtcgagtctg
     3781 gcgcgaccta cacctacacc tggaaggtgc cggagcgggc cggcccggcc atgcacgacc
     3841 cgagctcgat cgtctggatg taccactccc acgtcgatga gccggccgac acctacgccg
     3901 ggctgagcgg tccgatcatc atcactcgcg ccggtatggc gaaaccggat gggtcaccaa
     3961 ccgacgtcga tcgcgagttc gtgctcctct tcgaggtgat ggatgagaac gcgagccggt
     4021 acatcacgga caacgtcgcg tatcacatcc atcccgaagg agattcggac gagcaccagg
     4081 atttcgacga cgagttcatc gagagcaacc tgatgcacgg gatcaatggc tatgtctacg
     4141 gcaacctgcc gggcctgacc atgcgcgtcg gggagcgagt ccgctggtac gtgatgggca
     4201 tgggcacgga ggtcgatctc catacgcccc actggcatgg caacacgctg accatgatgg
     4261 gcatgcggac cgacatggtc gagctgttgc cgatgagcat gaaaacgctc gatatgacgc
     4321 cggacgccgt cgggacctgg ctcctccact gccacgtcaa cgaccacatc gtcgccggca
     4381 tgcaagcgct ctacaccgtc acggagtaac gaccctcgaa tcaggccggg accgccagac
     4441 ggccccggcc gggcgtccca tgcctacaat ggcatccgac cgaacggtag gagggatagc
     4501 aacgcgtggg gcagatcgat caacgaggga agttggctga ggagccattt tcgtatcgcc
     4561 gcaccaaggc cggcgatgtg ttcatcagct ggaacgggaa gtcagtaaca acgctcaagg
     4621 gcaaggcagc cgccacattt ctggcaaaga tcgccgacct cgacgacgag aaggcacaac
     4681 tcctgatggc caaagccacc ggccacttca aacacggcaa cgagcgcccc tccggtccgg
     4741 accgcgtctg aacccttcca tgcacgagaa gtgtcgcctc ttggagagag ttcgacgacc
     4801 gtgcgaatcc tgattacgtg cgaggttgcg tttactgatc ggtgaggaaa aggcgtgaca
     4861 cacgaaactg cggaggggtc gaacccggag ccgtttgagc cagttctctt gaagtgggag
     4921 ccttggcgtc cgcaagaggt cgcgcacttg ctcgcccagg ttgaggcacc gtggtacgtc
     4981 gctgccggtt gggcaataga cctcttcctc ggatgtccgc gccgtgcgca cgaggacatc
     5041 gagatcggag tgccgtagga ccggttcggc gagttcgccg cggcgctggc cggccacgaa
     5101 ctcttcgtcg ttggcggtgg tagcgcatgg ccgctggcac acgctggcga tgcgtacatt
     5161 gccaaccatc aaacgtgggt gcgcgagccc gccacaggac tgtggcggct cgacatcttc
     5221 cgcgagccgt cagacggggg ctcgtgggtc taccgccgta acacgt
//
LOCUS       DATAOZ010000358         5392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_933135, whole genome shotgun sequence.
ACCESSION   DATAOZ010000358 DATAOZ010000000
VERSION     DATAOZ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933135"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="VHR64_09150"
     CDS             <1..1598
                     /locus_tag="VHR64_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX3303034.1"
                     /translation="LLVFIGANPANNQPVTTKYMHYAKAAGTRMVTINTMRESGMERY
                     WIPSIPESAIFGTKLADDNFLISTGGDVAFLSGALKHMLERDLIDHAFVDGHTHGYDE
                     LVAQIDSLSWADLEQASGATTAQMRTFGEMVGRARTAVFVWSMGVTQHTYGEATVRAI
                     VNLALTRGFVGRDKCGLMPIRGHSGVQGGAEMGAYATAFPGGSPVNKETAARLSEQWG
                     FDVPSTPGLTAPDMIDAAAKGELDVLVAMGGNFREAMPDPRYVDKALGNIPLRVHIDI
                     MLSNQMLVDPADTVVLLPAMTRYEIPGGITQTSTERRIMFSPEIEGPRIGEARPEWQI
                     FTELAARIRPELADKIAFSATQAIRDEIARVVPAYEGIENLRETGDQVQYGGPHLGAG
                     WTFPTSDGRGSFSVVDLPNAAIPEGMFALATRRGKQFNTMVHETSDAMTGAGARDAVF
                     MHKGDATALGLANGDPVVLRNQFGELRGRIFLARIQPRNVQVYWPEGNVLLDHRQRSP
                     LSHVPDYNAFVTIEPVAVANAAD"
     gene            1600..2454
                     /gene="fdhD"
                     /locus_tag="VHR64_09155"
     CDS             1600..2454
                     /gene="fdhD"
                     /locus_tag="VHR64_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX3303035.1"
                     /translation="MDRRKGSKSRVRVTAVSGARSRQRSDWLATEEPLEIRVSDAGTL
                     DPLLTHVTMRTPGADFELAAGFLLAEGIIRRPEQIRQMEFCVGPAPTAIQQYNIISVE
                     VVDPDMRGRTDAYFRTAVSACGVCGKASLDELSLRGVEPVMTQVEVDQSVIERLPVLL
                     RERQRLFEATGGLHGAALITASGEVLAVREDVGRHNAVDKLLGWSLLNPGQATERAIL
                     MVSGRLSYELAQKAAVGRIPVLAGVSAPSSLAVELAREFGLTLIGFLRGDSFNIYAGQ
                     QRIRLLRA"
     gene            complement(2435..3526)
                     /locus_tag="VHR64_09160"
     CDS             complement(2435..3526)
                     /locus_tag="VHR64_09160"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX3303036.1"
                     /translation="MIDLAYRAAKPLLFRADPERVHDRVISALGLISRSPRALRVLDR
                     IGDEADPRLAVEFAGMRLPGPVGIAAGLDKNGVAFPALRGLGWDFVEIGTITPKPQPG
                     NSRPRVFRLLEDQALINRMGFPGAGADAIAMNVVQRRQRQVPIGCNVGPNKASVEAGL
                     DAVISDCRLLVSRFASLATYLVVNISSPNTAKLRDLQGKEALRELLTEVKAAIPADRP
                     KPLFVKIAPDLTDAEIDDLVGVVLDVGLSGIVATNTTIARPETLRSPRRPEIGGLSGA
                     PVRARSIEVIDRIARESSGSLPIMAVGGIASGADAIAALRTGASAVQVYTGMIYEGPG
                     LARQIKREILAEMDRTGAASLADLRRGAT"
     gene            complement(3557..4756)
                     /locus_tag="VHR64_09165"
     CDS             complement(3557..4756)
                     /locus_tag="VHR64_09165"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX3303037.1"
                     /translation="MSRLGLGAVLERLLAAARAALPTMAIAAALLAVMSSVLAPIASA
                     EDDGWTPPERVWVEETGQTVDKTFLTEWRDNMGLIGDPISQELKERVELDGVKTKERT
                     VQYYTNLALVSTYDDKRGDDWNVRALPLGIDALARDTTRLKNVKLPTSDKCKGFEDDE
                     CLYFKDTKHAVKLGFKSYWDAHQGEQLLGSALTEEFVAPDGWTTQYFENGVLLWKKDK
                     GVVPRALGKEAAARARISTRAVAQPNDVPVYAEALFSPPQPVAEVTLPQGPGPQQGTY
                     KEVVVSISQQYMWAYEDGVEVIESWVSTGTAETPEVTTPVGNWSILTKYDSQTMEGTI
                     SGQYYRVEDVPYVMYFDNLGNALHGTYWHNNFGHPMSHGCVNLPMDVAAFLYGWADVG
                     TAVTILP"
     gene            4909..>5392
                     /locus_tag="VHR64_09170"
     CDS             4909..>5392
                     /locus_tag="VHR64_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX3303038.1"
                     /translation="MSEPRRWRDPLTLESPPLSITGNRIVDEILVRRGFTSAKETREW
                     LTADLPRHCASAELSGMNLVVGRIARAIYAGEKFRVFGDYDCDGVTSSAIISGAVGCA
                     LAGRGSVDVELPSREQGYGLRPDVLDRAVADGVTLLVAVDCGSNDLELIGDAKGRGLD
                     V"
BASE COUNT          899 a         1708 c         1819 g          966 t
ORIGIN      
        1 acctgctggt cttcatcggc gcgaatccgg ccaacaacca gccggtcacg accaagtaca
       61 tgcactacgc caaggccgcc ggcacgcgaa tggtcacgat caacacgatg cgcgagtcgg
      121 gcatggagcg gtactggatc ccatcgatcc cggagagcgc gatcttcggc accaaactgg
      181 ccgatgacaa cttcctgatc tcgaccggag gagatgtcgc cttcctgtcc ggcgcgctca
      241 agcacatgct ggagcgggat ctgatcgatc acgcctttgt tgacggacac acgcacggct
      301 acgacgagct ggtcgcgcag attgactcgt tgagctgggc cgacctcgag caggcgtccg
      361 gcgccacaac ggcccagatg cgcacgttcg gcgaaatggt cgggagggca cggacggccg
      421 tcttcgtctg gagcatgggc gtcacccagc acacctatgg cgaggcgacg gtccgcgcga
      481 tcgtcaatct ggccctgacc aggggcttcg tgggtcgcga caaatgcggc ctgatgccga
      541 tccgcggtca ctcgggcgtg cagggcgggg ccgagatggg cgcctatgcc acggccttcc
      601 cgggcggttc gccagtcaac aaggagaccg ccgcacgtct cagcgagcag tggggcttcg
      661 acgtcccgtc gacgccgggt ctgaccgcgc cggacatgat cgatgccgcg gcgaagggcg
      721 aactggacgt gctggtcgcg atgggcggca acttccgcga ggcgatgccg gatccccggt
      781 atgtggacaa ggcgctcggc aacattccgc tgcgggtcca catcgacatc atgctctcga
      841 atcagatgct ggtcgatccg gccgacactg tcgtcctgct gccggcgatg acccggtacg
      901 agattccggg cgggatcacg cagacctcaa ccgagcggcg gatcatgttc agtcccgaga
      961 tcgagggacc gaggatcggc gaagcgcgtc cggagtggca gatttttacc gagctcgcgg
     1021 ctcgcattcg cccggagctg gccgacaaga tcgcgttctc ggccacgcag gcgatccgcg
     1081 atgagatcgc ccgggttgtc cctgcctatg agggcatcga gaacctgcgg gagaccggcg
     1141 accaggtcca gtacggagga ccgcacctcg gtgccggctg gaccttcccg acgtccgatg
     1201 gtcgcggttc attcagcgtt gtcgatctgc cgaatgcggc gatcccggaa ggcatgtttg
     1261 cgctggcgac ccggcgcggc aagcagttca acacgatggt tcacgagaca tcggacgcga
     1321 tgaccggagc cggcgcgcgc gatgccgtct tcatgcacaa aggcgacgcg acggccctcg
     1381 gactggccaa tggcgacccg gtcgtcttgc gcaaccagtt tggcgagctg cgtggccgca
     1441 tcttcctcgc ccggatccag ccgcgcaatg tgcaggtcta ctggccggag ggcaacgtgc
     1501 tgctcgatca ccggcagcgt tcgccgctct cgcacgtgcc ggattacaac gccttcgtga
     1561 ctatcgagcc tgttgcggtc gccaatgcgg ccgattgacg tggacaggcg caagggcagc
     1621 aagtcgaggg tccgggtcac ggcggtcagc ggagctcgga gccggcaacg atcggactgg
     1681 cttgcgaccg aggagccgct cgagatccgg gtcagcgatg ccgggacgct cgatccgctc
     1741 ctgacgcatg tgacgatgcg gacgcccggc gccgacttcg agctggcggc cgggttcctg
     1801 ctggccgagg gcatcatccg gcgaccggag cagatccggc agatggagtt ctgcgtcggt
     1861 ccggccccga cggcgatcca gcagtacaac atcatcagcg tcgaagtcgt cgaccccgat
     1921 atgcgcgggc ggaccgacgc gtattttcga accgccgtca gcgcatgcgg cgtctgcggc
     1981 aaggcgtcac tcgatgagct gtcgctgcgc ggcgtcgagc cagtgatgac tcaggtcgag
     2041 gtcgaccagt cggtcatcga acgtctgccg gtcctcctgc gcgagcggca gaggttgttc
     2101 gaggcgaccg gtgggctgca cggcgcggcg ctgatcaccg cgtcgggcga ggtgctggcg
     2161 gtgcgggagg acgttgggcg acataacgcc gtcgacaagc tcctcggctg gtcgctgctc
     2221 aatcccggtc aggcaaccga gcgggcgatc ctgatggtta gcggccggtt gagctatgag
     2281 ttggcgcaaa aagcggctgt cggccgcatt ccggtcctgg ctggagtctc ggcgcccagc
     2341 agcctggccg tagagctggc ccgcgaattc gggctgacgt tgatcggctt cctgcgcggc
     2401 gactcgttca acatttacgc gggccagcag cggatcaggt tgctccgcgc ctgagatcgg
     2461 ctaaagacgc agcgccggtg cgatccatct ccgcgaggat ttctcgcttg atttgccttg
     2521 ccaggcccgg accctcgtag atcatgcccg tgtagacctg cacggccgaa gctcccgtcc
     2581 tcagcgccgc gattgcgtcc gcaccggagg caattccgcc cacggccatg atcggcaggc
     2641 tgccgctgga ctctctggcg atccggtcga tgacctctat tgatcgggca cgaacgggcg
     2701 cgccactcag gccgccgatt tctggcctgc gcgggctgcg cagcgtctcg ggtcgcgcga
     2761 tggtcgtgtt ggtggcaacg attccgctca gccccacatc gagcacgacg ccgacgagat
     2821 cgtcgatttc ggcgtcggtc agatcgggcg cgatcttgac gaagagcggc ttcggccgat
     2881 cggctgggat cgctgccttg acctcggtca gcagctcccg cagtgcctct ttcccttgca
     2941 ggtcacgcag tttggcggtg ttcggcgacg agatgttgac caccaggtag gtggccagcg
     3001 acgcgaagcg gctgaccagc aaccgacaat cgctgatcac ggcatccagc ccggcctcga
     3061 cgctcgcctt gttcggtccg acattgcagc cgattggaac ctggcgttgg cggcgctgga
     3121 ccacgttcat cgcgatcgcg tcggcgccgg cgccggggaa gcccatccgg ttgatcagcg
     3181 cctggtcctc caggagccgg aagacgcgcg gtctcgagtt gccgggttgc ggtttgggcg
     3241 tgatcgtgcc gatctcgacg aagtcccagc cgagcccgcg aagtgctggg aaggcgaccc
     3301 cgttcttgtc gagcccggcg gcgataccaa ccgggccggg caatcgcatc cctgcaaatt
     3361 cgaccgccaa tcgcgggtcg gcttcgtcgc caattcggtc gaggacgcga agcgccctcg
     3421 gtgatcgact gatcaggccg agggcggaga tgacgcggtc gtgaactcgt tcggggtccg
     3481 cccggaagag cagtggcttg gcggcgcgat aggcaaggtc gatcacgccg tgacgatacc
     3541 acggcgtgat cgcgtcttac ggcaggatcg tgacggcggt gccgacgtcg gcccagccat
     3601 agaggaaggc ggcgacatcc atcggcaggt tgacgcagcc gtggctcatc gggtggccga
     3661 agttgttgtg ccagtaggtg ccatgcagcg cgttaccgag gttgtcgaag tacatgacgt
     3721 acggcacgtc ttcgacccgg taatactggc cgctgatcgt cccttccatc gtctgcgagt
     3781 cgtacttggt cagaatcgac cagttgccga ccggagtcgt cacctcgggc gtttcggcgg
     3841 tgccggtgct gacccaggac tcgatgacct cgactccgtc ctcataggcc cacatgtact
     3901 gctgcgagat cgagacgacg acctccttgt aggtgccttg ctgcggtccc gggccttgcg
     3961 gcagggtgac ctcggcaact ggctgtggcg gcgagaagag cgcctcggca tagaccggaa
     4021 catcgttcgg ctgcgcgacg gccctggtgc tgatcctggc ccgggcggcg gcttccttgc
     4081 cgagcgcgcg cgggacgact cccttgtcct tcttccaaag caggacgcca ttctcgaagt
     4141 actgcgtggt ccagccgtcc ggcgcgacga actcttccgt cagcgccgag ccaagcagct
     4201 gctcgccctg gtgcgcgtcc cagtagctct tgaagccgag tttgaccgcg tgctttgtgt
     4261 ccttgaagta gaggcactcg tcgtcctcga agcccttgca cttgtcgctc gtcggtagct
     4321 tgacgttctt caatcgggtc gtgtcacgag cgagcgcgtc gattccgagg ggcagcgcgc
     4381 ggacattcca gtcgtcacca cgcttgtcgt cgtaggtcga gaccagggca aggttcgtgt
     4441 agtactggac ggtgcgctct ttggtcttga cgccgtcgag ctcgacccgc tccttcaact
     4501 cctggctgat cggatcgccg atcaggccca tgttgtcgcg ccactcagtc aggaaggtct
     4561 tgtcgactgt ctggccggtc tcttcgaccc agacgcgttc gggcggcgtc cagccgtcgt
     4621 cctcggccga ggcaatcggc gccagcacac tggacatgac ggcgagcagc gcggcggcga
     4681 tcgccatcgt gggcagcgcg gcacgggccg cggcgagcag gcgctcgaga acagcgccca
     4741 atccgagacg cgacatcgat ctctccttgt gcgtgatgga cacgatgata acaagctcct
     4801 gatcgaggaa cctgggcggt aacaggaccc gaatagtttg gggtaggccg gtataatatc
     4861 acgagccgtg aacgtacagt tcagccgttg actagccgaa aaggtccaat gtccgagccg
     4921 cgccgctggc gagacccact cacgctcgaa tctcccccgt tgtccatcac tggcaatcgt
     4981 atcgtcgacg agatcctggt ccggcgcggc ttcacctccg ccaaagaaac acgcgagtgg
     5041 ctgacggcgg atctgccgcg gcattgcgcc tcagccgagc tcagcgggat gaatctggtc
     5101 gtcggccgga ttgcccgggc gatctatgca ggcgagaagt ttcgcgtctt tggcgactac
     5161 gactgcgatg gcgtgacctc ctccgccatc atctccgggg ccgttggatg cgcgctcgcc
     5221 gggcgtggat cggtcgatgt cgagctgccg agccgggagc agggatatgg tttgcggccg
     5281 gacgtgctcg accgcgcggt cgcggacgga gtgacgctcc tggttgcggt cgactgcggc
     5341 agcaatgacc tggaattgat cggcgacgcc aaagggcggg ggctcgacgt gg
//
LOCUS       DATAOZ010000359         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3040722, whole genome shotgun sequence.
ACCESSION   DATAOZ010000359 DATAOZ010000000
VERSION     DATAOZ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3040722"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1103)
                     /locus_tag="VHR64_09175"
     CDS             complement(105..1103)
                     /locus_tag="VHR64_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HEX3303039.1"
                     /translation="MSDAPPKQPSERLEKWVKRLSFAAMIGMFLVLMMGSTVTNTGSA
                     EGCGRHWPLCQGEFIPSYAVTTAIEFSHRVVTAVEGVLILAVSIGAILIRRGNRQVKV
                     LVAVLIATLILQSGMGAAAVMWPQSPVIMASHFGISMTCFAAIFLLTRYLYEPSSLDG
                     DRSVRKTPPPLWFRRGVWASLVLSVFVAYVGAYMRHSGNELGCHTWPLCNGQVFPGFT
                     GSEGISFSHRLLAGSLMLLIFGLAFWARSFKTDYPELASVLNVASVAIVGQALAGAFV
                     VNTRLDLWTTLLHAALMAILFVMICDACRIVWSARPRSTRAAVPDARQTSALPAGD"
     gene            1229..>1656
                     /locus_tag="VHR64_09180"
     CDS             1229..>1656
                     /locus_tag="VHR64_09180"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEX3303040.1"
                     /translation="MPIRTVEATRYVTPLREGGSVPAIVEADDDGLYVLKFHGAAQGP
                     RSLVAELISGEIGRTLGLPVPEIVLIELDPVLAKAEPDPEIQELIEKSGGLNLGLDFL
                     PGSLAFAPAAPPEINPELAAQVVWFDAYTLNVDRTPRNPNL"
BASE COUNT          355 a          518 c          509 g          274 t
ORIGIN      
        1 gacctacacc ctgaccattg atgaaccggg aacctacgag tttttctgtg aattccatcc
       61 ggcgatgaca gggacgatca tcgccagcgc atagccggca agcgttagtc gccggcaggg
      121 agagcgctgg tctgccgcgc atcggggacc gccgcgcggg tcgagcgcgg ccgagcgctc
      181 cagacaatgc ggcatgcatc gcagatcatg acgaagagaa tggccatcag cgccgcatgc
      241 agtaatgtgg tccagagatc cagccgggta ttcaccacga atgcaccggc cagcgcctgc
      301 ccgacgatcg ccaccgaggc gacattcaat accgaggcaa gctcgggata gtcggtcttg
      361 aatgaccgcg cccagaaggc cagaccgaag atcagcagca tcagcgatcc cgccagcagg
      421 cgatgggaaa acgagattcc ttccgatccg gtgaaaccgg gaaagacctg cccattgcac
      481 agcggccagg tgtggcagcc gagctcgttg cccgaatggc gcatataggc gccgacatag
      541 gcgacgaata ccgaaagcac gagtgaggcc cagacgccgc ggcgaaacca gaggggcggt
      601 ggagtcttgc ggaccgatcg gtcgccatcg agcgacgacg gctcatagag atatctggtc
      661 aaaaggaaga tcgcggcgaa acacgtcatg gagatgccga aatggctcgc catgatcacc
      721 ggcgactggg gccacatcac cgcggctgcg cccatgcccg attgcaagat cagcgtggcg
      781 atcaacaccg cgaccagcac cttgacctgg cgattgccac gccggatcag gatcgctcca
      841 atcgaaaccg cgagaatcaa caccccctcg accgcggtga cgacccgatg actgaactcg
      901 atggcggtcg tgacggcgta ggatggaatg aattcgccct ggcagagagg ccagtgacgc
      961 ccgcagcctt cggctgatcc ggtgttggtg acagtcgatc ccatcatcag cacgaggaac
     1021 atgccgatca tggccgcaaa cgacaggcgt ttgacccatt tctcgagtcg ttcgctcggt
     1081 tgtttgggag gtgcgtcact catcggctcg gttccttctc ggcatgaggg taccatccgg
     1141 ggccacccac tggcatccct gcatcgccgt acgcggcttg ccacctatac tgtcgcgctg
     1201 gcccgatttt gggagcacgg agacaccgat gcccatccgc accgtcgaag cgacgcgcta
     1261 tgtgacgcct ttgcgtgagg gtggatcagt acccgctatc gtcgaagcgg acgatgatgg
     1321 actctacgta ctcaagttcc atggcgcagc gcaaggacca cgatcgctgg tcgccgaatt
     1381 gatcagcggc gagatcggcc gaacgctcgg tttgccggtg ccggagatcg tgctaatcga
     1441 gctcgatccg gtgctggcca aggccgagcc ggatcccgaa atccaggagc tgatcgagaa
     1501 gagcggcggc ctcaatctgg gactcgattt tttgcccggc tcgctggcct tcgcgcccgc
     1561 cgctccgccg gagatcaacc cagagctggc cgcacaggtc gtctggttcg acgcgtacac
     1621 cctcaatgtc gaccggaccc cgcgcaaccc gaatct
//
LOCUS       DATAOZ010000360         6194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2513382, whole genome shotgun sequence.
ACCESSION   DATAOZ010000360 DATAOZ010000000
VERSION     DATAOZ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2513382"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VHR64_09185"
     CDS             complement(<1..468)
                     /locus_tag="VHR64_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3303041.1"
                     /translation="MTGTVLVGAEATDDQLVRAAQHALPAFEHLYRRYVNDVFRFCCR
                     RLNSEADAADATSVIFTRALANISGCQAQSFRPWLFTIARNVVTDHYRAARPVETLDE
                     SLEVQDQAVGPEELAISGDERRTLTAALGQLTEEQRSIIELRLAGLSANEIATA"
     gene            751..2376
                     /locus_tag="VHR64_09190"
     CDS             751..2376
                     /locus_tag="VHR64_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303042.1"
                     /translation="MSHPNGRPAPSRSEPEHNPWIILIVLCTAVFMLLLDTTIVNNAQ
                     VKIREGLNANLTQIQWVLDSYILTYAVLLLSLGRLGDIFGRKRLFILGMAIFTIASGL
                     CGISSWLGEQIGVSGINMLIAARVLQGVGGAMMMPQSLSLLTVNFPPEKRGTAMGIWG
                     SIVGLGAVAGPLIGGYLVTHYAWEWVFLINLPVGVVALIAAARIIPESTDPHASRKLD
                     WTGLILSGVGIFLFVYGIIEGNIKGWGNPQIYGSIILGLILIAVFVLWEIHVPDPMMK
                     VELFRIRNFFIGNVVALAVSFGMLGIFFPMTIYLQGILGYSAIKAGLAMSPMSITILF
                     SAPFSGRLSDRIGTRWILFGGLLTMVVGILAIVRASTLDATPWTLLPSLVLTGLGMGF
                     TFAPMTAAVMRDVPPRIAGSASGILNTMRNIGQVLGIAVLGSVLQHQVSVHVDNNLQP
                     IVTDSGTRNQIVEYAQQSQFERIAGLVPTEQLAAVFDAVRSGFTSSVHDTFTAGALIC
                     LVAAAVAFFLRDPVREQLPQPAEREVATPVAAD"
     gene            2636..3688
                     /locus_tag="VHR64_09195"
     CDS             2636..3688
                     /locus_tag="VHR64_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX3303043.1"
                     /translation="MPSIRIPVTPIMGTADRRSLLKMAAALGIAAPAAGFGADDMRAQ
                     EHDHATPVPGGIPQGGTGAATPIPGEISPFKRYDPILPPAQAGPHDIVVEAKDQTLYV
                     AKDVPYAAWTFDGTVPGKIFRVTEGDTVNFTLKNSAHMAHSMDFHSAKTPPNVNYKTI
                     NPGEEFSWSFTATTPGAYMYHCGTPPVLMHIGAGMYSAMIVDPKEGWSPAQELIFVQS
                     EYYLIDGENGIKVPDFKKMMTLNFMDYTVFNGHATQYVDEPIDVKVGELIRIFVVNAG
                     PNVWSSFHVVGAIFDKAYINAHPHNELRGLQSITIGPGDGACVEFTVEEPGHYVAVNH
                     AFGHATHGAVAVLNAT"
     gene            3704..4069
                     /locus_tag="VHR64_09200"
     CDS             3704..4069
                     /locus_tag="VHR64_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3303044.1"
                     /translation="MTGPMTDTYISLAEQLEKIPLHESGKRSLILTKTEQLRVILQRM
                     STGAELREHSAPGQITLQGLSGRFTVSVGGDTFEVTDDSLITLGPGIRHNVVCDEDGA
                     FLLTIAWPHGPDPVSSGEA"
     gene            4076..4543
                     /locus_tag="VHR64_09205"
     CDS             4076..4543
                     /locus_tag="VHR64_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303045.1"
                     /translation="MDRLTSIMLRLALVWLMVGFVIGGLMLTDRKVPGDWRLWLSPGH
                     GHMLFVGWFLQFAVGVAYWLLPRKRTPQQPIGYAERPAWVAVAALNVGLALRVGGEAA
                     ERSGHASDLTFVILAIAAVLQIVAAAIFVTQLWPRVAARPIRSGSSSTKGAPT"
     gene            4540..4761
                     /locus_tag="VHR64_09210"
     CDS             4540..4761
                     /locus_tag="VHR64_09210"
                     /inference="COORDINATES: protein motif:HMM:NF016303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HEX3303046.1"
                     /translation="MTETTQTPITDILIRDLVERYPNTMGVLAPLGIDLCCGGGHPLG
                     EALDLHEIDRASVMPQIEQIVATQDSTKG"
     gene            4766..6046
                     /locus_tag="VHR64_09215"
     CDS             4766..6046
                     /locus_tag="VHR64_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303047.1"
                     /translation="MGRHLVPATILAATLAFAVAALTAGLVPYRGWWWNPAVRLAVVG
                     GITPMIYAVNIRIVPVFSRRNWRSETLLRAQIILTVAGAWLLFFGGTERNRDVVTTGV
                     LLAWVGGVLFLVNTFALFRQEPLKNRPAPPLPYPEHRAIDKIATRFTSLSGLFILLGL
                     SIGVYVQLWPPRTGRWDLVWAHTMLVGFFLSMVSGVCYHVLARWTGHPWQSLRLIQIH
                     FYVTLIALPLMLIALATNDQTLFSYAGPIQGLALWLFLWNIAPMVWRMTGPTRIALVL
                     AIAALLAGVFLGAMFAGHPVVGVHYRIVHADLNLFGWTGLLISGVAYYLVPRFFGRPL
                     RWPRLAAIQVGLLAAGITASALAWTWRIEKGGASDLITASQSVVAIGFVLLGVIVSGT
                     LAFGKSKSGQATVSAVPMVKQFKRPQPPLQPTGN"
BASE COUNT         1019 a         2087 c         1899 g         1189 t
ORIGIN      
        1 cgcggtcgcg atctcgttcg cgctcagtcc ggccaggcgg agctctatga tcgagcgctg
       61 ctcctcggtc aactgaccga gcgcggcggt caacgtccgg cgctcgtcgc ccgagatggc
      121 cagctcttcc ggtccgaccg cctgatcctg cacctcgaga ctctcgtcca gcgtctcgac
      181 cggccgggcg gcacgatagt gatcggtgac tacgttcctg gcgatcgtga agagccacgg
      241 ccgaaaggac tgcgcctggc acccgctgat attcgccagc gcgcgcgtaa agatcacgct
      301 ggtggcgtcg gccgcgtcgg cttccgagtt gagccgccgg caacagaacc ggaatacgtc
      361 gttgacgtag cgccggtaga gatgctcgaa ggccggcaac gcgtgttgcg ccgcccggac
      421 gagctgatcg tccgttgcct ccgctccgac taacacagtt ccggtcatag cctccacgct
      481 gtcgccttca agcgatcatc aacctctccg gaataaggag acggcattcc cgccattctc
      541 ttacgggtcc cccgcgaagg cgaaaatcct gcccgaccag gtgggttact taccggtcgg
      601 aaagtgtgta tactgaacct cccgatgcgg ccggcattca ggttcggaat gtcccgaccg
      661 cccgggtcca ctcgatgacg agatccgttt cccttgttca tggcagcgcc ggcgaagaga
      721 gccgcgcgcc ggtcatggag gagttcgttt gtgagccacc cgaacggtcg tcccgcaccg
      781 tcacgatcag agcccgagca caacccctgg atcattctga tcgtcctctg tacggcagtc
      841 ttcatgctgc tgctggacac cacgatcgtc aacaatgccc aggtcaagat ccgcgagggg
      901 ctgaacgcaa acctgaccca gatccagtgg gtcctcgact cctacatcct gacctatgca
      961 gtactcctcc tctcgctcgg ccggctgggc gacatcttcg ggcgcaagcg actcttcatc
     1021 ctcggcatgg cgatcttcac gatcgcgtcg ggcctctgcg gcatttcctc ctggctcggc
     1081 gagcagatcg gtgtctccgg catcaacatg ctgatcgcgg ctcgcgtgct gcagggcgtc
     1141 ggcggcgcga tgatgatgcc gcagtctctc tccctcctga cggtcaactt cccgcccgag
     1201 aagcgcggca ccgcgatggg catctggggc agcatcgtcg gtctcggcgc ggtggcaggt
     1261 ccgctgatcg gcggctacct ggtcacgcac tacgcctggg aatgggtctt cctgatcaac
     1321 ctgccggtcg gcgtggtcgc cctgatcgcc gccgcccgga tcatccccga atcgactgac
     1381 ccgcacgcca gccgcaagct ggattggacc ggtctgatcc tctccggtgt cggcatcttc
     1441 ctcttcgtct acgggatcat cgaaggcaat atcaagggct ggggcaaccc gcagatctac
     1501 ggttcaatca tcctgggtct gatcctgatc gcggtcttcg tcctctggga gatccacgtt
     1561 cccgatccca tgatgaaggt cgagctcttc cggatccgca acttcttcat cggtaacgtg
     1621 gtcgcgctag cagtctcgtt cggcatgctc ggcatcttct tcccgatgac gatctacctc
     1681 cagggcatcc ttggttattc cgcgatcaag gcgggtctgg cgatgtcgcc gatgtcgatc
     1741 acgatcctct tctcggcgcc attctcggga cggctcagcg accgcatcgg cacgcgctgg
     1801 atcctcttcg gcggtctgct gaccatggtg gtcgggatcc tcgccatcgt ccgcgcctcc
     1861 acgctcgacg cgacgccgtg gacgctgctc ccttcgctgg tgctgaccgg cttgggcatg
     1921 ggcttcacct tcgcgccgat gaccgcggcc gtgatgcggg acgtcccgcc gcgcatcgcc
     1981 ggcagcgcct ccggcatcct caatacaatg cgcaacatcg gtcaggtcct gggaatcgcg
     2041 gtgctgggat cggtcctgca acatcaggtc tcggtccacg tcgacaacaa cctgcagccg
     2101 atcgtgaccg acagcggcac gcgcaaccag atcgtcgagt acgcgcagca gagccaattc
     2161 gagcgaatcg ccggcctggt gccgacagaa cagcttgcgg ccgtcttcga cgcggtccgg
     2221 tcgggcttca ccagcagtgt gcacgacacc ttcaccgccg gagctctgat ctgcctggtc
     2281 gccgcggcgg tcgccttctt cctccgcgac ccggttcggg aacaactgcc gcagccggcc
     2341 gagcgagagg tcgccacacc agtcgccgct gattgatcag cgaatacgta tcgtcctgcg
     2401 attgacacga atttgtaact ctgatcattc agatcagaca ttttcgcatt gcttctcgcg
     2461 tactctgtac tcatggatgc gggggctgct tttccttcct gccagcgctt cttcggagcg
     2521 gccctctgca tcccgttgtt tgtgcgtcag gcgcaggtgc cggccgacat tgggacggat
     2581 cggcgcgcca ggaggaaggc ggggtcgtga ccgattccgt caggaaggag acagcatgcc
     2641 gtcgatacgc attccagtaa ccccgatcat ggggaccgcc gatcgccgct cgctcttgaa
     2701 aatggccgcg gcgctcggta tcgcggcgcc ggccgccggc ttcggcgcgg acgacatgcg
     2761 cgcccaggag catgatcacg cgaccccggt tccgggaggg attccgcagg ggggaaccgg
     2821 cgcggcgacg ccaattcctg gcgaaatttc gccgttcaag cgctatgacc ctatcctccc
     2881 gccggcgcag gctggtccgc acgacatcgt ggtcgaggcg aaggatcaga ctctctacgt
     2941 cgcgaaagac gtgccgtacg cggcctggac cttcgatggc acggtccctg gcaagatctt
     3001 ccgcgtgacc gagggtgaca cggtcaactt cacgctcaag aactcggcgc acatggctca
     3061 ctcgatggac ttccactcgg ccaagacgcc gccgaacgtg aactacaaga ccatcaaccc
     3121 gggcgaggag ttctcgtggt cgttcacggc aacgaccccc ggcgcctata tgtaccactg
     3181 cgggaccccg ccggtcctga tgcacatcgg tgccggcatg tacagcgcaa tgatcgtcga
     3241 cccgaaagag ggctggtcac ctgcccagga gctcatcttc gtccaaagtg agtattacct
     3301 gatcgacggc gagaacggca tcaaggtacc cgacttcaag aagatgatga ccctgaactt
     3361 catggattac acggtcttca acgggcacgc gacgcagtat gtcgacgagc cgatcgacgt
     3421 gaaggtcggc gagctgatcc gcatcttcgt ggtcaatgcc ggtccgaacg tctggtccag
     3481 cttccatgtc gtcggcgcga tcttcgacaa ggcctacatc aacgcgcatc cgcacaacga
     3541 gctgcgtggc ctccagtcga ttacgatcgg ccctggtgac ggcgcctgcg tcgagttcac
     3601 ggtcgaagaa ccgggccatt acgttgcggt caaccacgcg ttcggccacg ccacgcatgg
     3661 agcggttgcc gttctgaacg cgacgtagcc cgggagaagc agcgtgacgg gtccgatgac
     3721 cgatacctat atctcgcttg ccgagcagct ggagaagatc ccgctgcacg agtccggtaa
     3781 gcggtccctg atcctgacca agaccgaaca gctccgcgtc atcctccaac gcatgagcac
     3841 cggcgccgag ctgcgcgagc actcggcgcc cgggcaaatc acgctgcagg gattgagcgg
     3901 ccggttcact gtctcggttg gcggcgacac gttcgaggtc accgacgact cgctgatcac
     3961 gctcggaccg ggcatccggc acaacgtggt ctgcgatgag gatggcgcgt tcctgctcac
     4021 catcgcctgg ccacacggac ctgacccggt ctcgtctgga gaggcgtagc ggcgagtgga
     4081 tcgtctgacg agcatcatgc tccggttggc gttggtctgg ctgatggtcg gattcgtgat
     4141 cggcgggctg atgctgaccg atcgcaaggt gcctggggac tggcggctct ggctgtcccc
     4201 cgggcacggg cacatgctgt tcgtcggctg gttcctgcag ttcgcggtcg gtgtcgccta
     4261 ctggctcctc ccccgcaagc gaacgccgca gcagccgatt ggatatgccg agcgtccggc
     4321 ctgggtggcc gtggccgcgc tcaatgtcgg gctggcgttg cgggttggtg gcgaggcggc
     4381 ggagcgatcc ggccatgcca gcgacctgac cttcgtcatc ctggcgatcg ccgccgtgct
     4441 gcagatcgtt gcggcggcga tcttcgtaac ccagctctgg ccgcgtgtgg cggccaggcc
     4501 gattcgttcc ggctcgtcgt ctacgaaagg agctccaaca tgaccgagac gacccagacc
     4561 ccgattaccg acattctcat ccgcgacctc gtcgagcgat accccaacac gatgggcgtg
     4621 ctcgcaccgc tcgggatcga cctctgctgc ggcggcggtc acccgctcgg cgaagcgctc
     4681 gacctgcacg agatcgaccg cgcgagcgtc atgccgcaga tcgagcagat cgtggccacg
     4741 caggacagca cgaagggata gcaccgtggg gcggcacctc gtaccagcga caatcctcgc
     4801 ggcgacgctg gcattcgcgg tcgccgccct gaccgccggc ctggttccct accgcggctg
     4861 gtggtggaac ccggccgtcc ggctcgcggt cgtcggcggg atcacgccga tgatctacgc
     4921 cgtcaacatc cggatcgtgc cggtcttctc ccggcgcaac tggcgctcgg agacgttgct
     4981 gcgggcgcag atcatcttga cggtcgccgg agcatggctg ctcttcttcg gcggcaccga
     5041 gcggaaccga gacgtcgtca cgacaggcgt tctcctcgcc tgggtcggcg gcgtcctgtt
     5101 cctcgtcaac accttcgcgc tcttccggca ggagccactc aagaaccggc ccgcgccgcc
     5161 cctgccctac cccgagcacc gcgccatcga caagatcgcc acccgcttca cctcgctctc
     5221 ggggctgttc atcctgctcg gcctgagcat cggtgtctat gttcagctct ggccgcctcg
     5281 aaccggccgc tgggacctcg tctgggcgca cacgatgctg gtcggcttct tcctctcgat
     5341 ggtctccggc gtctgctacc acgtcctggc ccgctggacc ggccacccgt ggcagtcgct
     5401 ccgactgatc cagattcact tttatgtcac gctgatcgcg ttgccgctaa tgctgatcgc
     5461 gctcgccacc aatgatcaaa cgctcttcag ctacgccggt ccgatccagg ggctcgcgct
     5521 ctggctcttc ctctggaaca tcgccccgat ggtctggcgc atgaccggcc cgacccggat
     5581 tgcgctcgtg ctggcgatcg ccgcgctcct cgccggcgtc tttctgggcg cgatgtttgc
     5641 cgggcatccg gtcgtcgggg tccattaccg catcgtgcac gccgatctca acctcttcgg
     5701 gtggacagga ttgctgatct ccggcgtcgc ctactatctg gtgccgcgct tcttcggccg
     5761 gcccctgcgc tggccccggc tggcggcgat ccaggtcggg ttgttggcgg ccggtattac
     5821 ggcgagcgcg ctggcctgga cctggcggat tgagaagggc ggcgcgagcg acctaatcac
     5881 cgcgagccag agcgtcgtcg cgatcggctt cgtcctgctc ggcgtgatcg tctccgggac
     5941 gctcgccttc ggtaaatcga agtcgggcca ggcgacggtc tcggcagtac ccatggtcaa
     6001 gcagttcaag cgcccacaac caccgctcca gccaaccggc aactgatctg cccattttgc
     6061 gactttggga cttccggcgg tcccgggcct ggtgtgcaat atgcaaagtc gtgtacgtac
     6121 tatatgattg cgcaagttca gccgggccag ggtggccagg agcacagccg gaatgacaga
     6181 gaaccaaccc gatc
//
LOCUS       DATAOZ010000361         4890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7515235, whole genome shotgun sequence.
ACCESSION   DATAOZ010000361 DATAOZ010000000
VERSION     DATAOZ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7515235"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VHR64_09220"
     CDS             complement(<1..583)
                     /locus_tag="VHR64_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303048.1"
                     /translation="MPSDTRADLVLDRFWDALVDDPMAGSDDLDLQAAASLRRLHAMA
                     SAPAPTVSRERVRHRLSGLAGADHEHAAPPADRPMPLLSLRRNAAAADGHPTTPTARS
                     LAAPQAIHGARRWTAILATAAIVLLALAASIRGLSPAFLGDDGKAPAVSAPNGQSGVT
                     ERMLLDIALTAEESAFSGQYGTQMGYFSSPPGSH"
     gene            645..1166
                     /locus_tag="VHR64_09225"
     CDS             645..1166
                     /locus_tag="VHR64_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303049.1"
                     /translation="MFLANGFMAAAEDARDFGIFQPGQAQLEQLALIRPQLRQQRMDE
                     LMIVRSNRRLFGRWGRIPHGHGRFEWIDRVPAAIAMTIGYHVAGNGEKPAPEPGVVPG
                     WLKLSEVGEHAHEYIARGVLGLFPVAKPVKAEAQDRIEIAIVKGPKCRVIALGSEDEQ
                     RVGTNRRWPLVRC"
     gene            complement(1312..1791)
                     /locus_tag="VHR64_09230"
     CDS             complement(1312..1791)
                     /locus_tag="VHR64_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX3303050.1"
                     /translation="MVNHTHQPAISDLAKRISVVAMIGIALGAGLMAEAAQNSSGTPS
                     PSPAASPSPAGSPSPISLAGHGSATVSHDVTIPLTDEGYVPNYVQSTSGHDLNVTLVN
                     TGSRRHGFTIAELGVDVMLDPGQTKTLVVHPVGLGDYTYVSTAPGDDGMTGTLTFYI"
     gene            complement(1851..3602)
                     /locus_tag="VHR64_09235"
     CDS             complement(1851..3602)
                     /locus_tag="VHR64_09235"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3303051.1"
                     /translation="MRTRTLVALVVILTLLIPGFGLLAPSSPTPARAQDQGQSLPDNP
                     DFQGVATPGGTIPNNPKIQLVKVAGGLADPINIASANDGSGRIFVVERIGRIRIVDKD
                     GKLLPDPFLDIQSLVKTDFLEQGLLGLAFSPDYKTNGLFYVYYSDYQTNGDVVLSEYK
                     VSSDDPNKADPKSARILFTQDKPYVNHNGGTLRFGPDKMLYISIGDGGLAGDPYDNAQ
                     RTDVLLGKLLRIDPNARDAGAYGIPKDNPFANTGVVLPSDQASDMAQDGSYHPAARRE
                     ICNWGLRNPWQFNFDPKTGELYLDDVGQNAWEEVDVFPANQPCNWNLGWDHNEGASCY
                     PPGSKCLQAGALPVATYNHKSGDCSITGIGVYRGSESTNLDGIYFNSDFCSGKIYGLQ
                     MDSSGAWQYQVLLQTGLSVTGSGQDENGELYVTSCSCKYSRDYNALDNPGGAVWRIVS
                     ADKVPQGAELAPSPSATAGTPEAAATPQAATPAASPVAGGGQASTQQSVDLVDIAFQP
                     NELTIPAGQDVTITLTNKGKLPHTFTIDQLGVDSGPVQPGDTATVKINAQAGDYQYYC
                     KTPGHKEAGMVGTLHVK"
     gene            4112..4810
                     /locus_tag="VHR64_09240"
     CDS             4112..4810
                     /locus_tag="VHR64_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3303052.1"
                     /translation="MPMTFLVAEESSDTAHGIEFSAQMSWPGCRVITAGTGAEAIARF
                     AEAPADAVLLDVQLEPSDGFEVCRQIREMSNVPIVMLSADDAIVDKIRAFDLGADDYL
                     VKPFDHLELMARLRALLRRAEMGSAHAIGEYACGDLAIDFGLHMVRCHGEEVRLTSTE
                     FRLLEVLVRNAGMTMPHHVLLDRVWGSEWGGRPGYLKVFVRRLRQKLDDDAERPRYIE
                     TVWGTGYRFLPYSV"
BASE COUNT          856 a         1451 c         1655 g          928 t
ORIGIN      
        1 cgtgagaacc cggcggcgag ctgaaatacc ccatttgcgt gccatattga ccgctgaatg
       61 ctgattcctc tgcagtcagc gcgatgtcca gcagcattcg ctccgtgacg ccggattggc
      121 catttggcgc cgaaaccgcc ggggcctttc cgtcgtcgcc gaggaaggcg ggactcaggc
      181 cgcggatcga tgctgccagg gcgagcagga cgattgcggc ggtcgccagg atggcggtcc
      241 aacggcgcgc tccgtggatc gcctgcggtg cggcgaggga gcgcgccgtt ggcgtggttg
      301 gatgaccatc cgcagccgct gcgttccgcc tcagggacaa caggggcatc ggacgatcgg
      361 cgggtggagc agcgtgttca tggtctgctc ccgccagccc gctcaggcga tggcgcaccc
      421 gctcccggct gaccgtgggt gcgggcgcgg atgccatggc atgtagccgc ctcagagatg
      481 cggccgcctg caggtcgagg tcgtctgaac cggccatggg gtcgtcgacg agggcgtccc
      541 agaaacgatc cagcaccagg tcggcgcggg tatcgctagg catgaagcac cccctgttcc
      601 gcctgctgcc cgacaaggac gcggagtgac tggacgatgc gggattgttc cttgcgaacg
      661 gcttcatggc tgcggccgag gatgcgcgcg atttcggcat ttttcagccc ggccaggcgc
      721 agctcgagca gctcgcgttg atccggcctc aactgcgtca gcaacgcatg gatgaattga
      781 tgatcgtccg aagcaatcgc cgtctcttcg ggcgttgggg tcgaatcccg catggtcatg
      841 gccgcttcga gtggatcgac cgggtgccgg cagcgatcgc gatgacaatc ggctaccacg
      901 ttgcgggcaa tggtgaaaag ccagcacctg aacccggcgt cgtgcccggc tggctgaaat
      961 tgagcgaggt tggcgagcac gcgcacgaat acatcgcccg tggcgtcctc ggcctgttcc
     1021 cagttgccaa gccggtaaaa gcagaagcgc aggaccgcat cgagatagcg atcgtaaagg
     1081 gccccaaatg cagagtgatc gcgctgggca gcgaggacga gcagcgcgtc ggaacaaacc
     1141 gacggtggcc actggtccgt tgctgattct ggcgtcgatg ccctcatcac tgcgtcccca
     1201 tttgccaatc gcgtacgcaa gtagaaacgc tgccgcagaa aatatgtgac caggcaaagg
     1261 aggtacgccc agaatacgac cagggcgcaa tggcagagct tgaacctggg ctcagatgta
     1321 gaaggtgagg gtgccggtca tgccgtcgtc acccggcgcg gtgctgacgt aggtgtagtc
     1381 gcccagaccc acgggatgaa ccacgagcgt cttcgtctgc ccgggatcga gcatgacgtc
     1441 gacaccgagc tcggcaatgg tgaagccgtg ccggcgggag cctgtattga cgagcgtcac
     1501 gttcaggtcg tgaccgctgg tgctctggac ataattgggg acgtagccct catccgttag
     1561 cgggatcgtg acgtcgtggc tgacggtcgc cgatccatgg cccgcgagcg agattggact
     1621 cggcgatccg gccggagatg ggctggctgc gggcgaaggg cttggggtgc cgctggagtt
     1681 ctgtgcggcc tccgccatga ggccggcacc gagggcgatc ccgatcatgg ctacgacgga
     1741 aatccgcttc gccaggtcac tgattgctgg ttggtgggtg tgattaacca tgagcgagga
     1801 ggccgctgcc cgggcccctt ggaccggggc ggcggctgtc cagatcggcc ctatttcacg
     1861 tggagggtgc cgaccatccc ggcctccttg tgaccggggg tcttgcagta gtactggtag
     1921 tcgccggcct gggcgttgat cttgaccgtc gcggtgtcgc ccggctgaac tggcccggag
     1981 tcgacgccca actggtcgat cgtgaacgtg tgcggcagct tccccttgtt ggtcagagtg
     2041 atcgtcacgt cctgaccagc cgggattgtc agttcattgg gctggaatgc aatgtcgacg
     2101 aggtcgaccg attgctgcgt gctggcctgc ccgccacctg cgaccgggct ggccgcgggc
     2161 gtggccgcct gcggagtcgc agccgcttcg ggcgttccgg ccgtggccga cggagacggc
     2221 gcaagttcgg cgccctgcgg caccttgtca gcgctgacga tccgccacac cgcgccaccc
     2281 ggattgtcga gcgcgttgta atcgcgcgag tacttgcacg agcatgatgt cacgtacagc
     2341 tcgccgttct cgtcctgacc tgatccggtc acgctcaggc cggtctggag caggacctgg
     2401 tattgccagg ctccgctact gtccatctgc aggccataga tcttgcctga acagaagtcc
     2461 gagttgaaat agatcccgtc caggttcgtc gattccgagc cacgatagac gccgatgccg
     2521 gtgatcgagc agtctccact cttgtggttg taggtcgcga ccggaagtgc gccggcctgc
     2581 aagcacttcg agcccggggg atagcaggac gcgccctcgt tgtggtccca gccgaggttc
     2641 cagttgcatg gctggttggc cgggaagacg tcgacttctt cccacgcgtt ctgtcccacg
     2701 tcatcgaggt agagctcgcc cgtcttcggg tcgaagttga actgccacgg gttgcgcagg
     2761 ccccagttgc agatctcgcg ccgcgcggcg ggatggtacg agccgtcctg ggccatgtcg
     2821 ctcgcctgat cgctcggcag cacaacgccg gtgttcgcga acggattgtc cttggggatg
     2881 ccgtaggccc cggcatcgcg cgcattcgga tcgatccgca ggagcttgcc aagcaacaca
     2941 tcggttcgct gggcattgtc gtacgggtcg ccagccaggc cgccgtcgcc gatcgagatg
     3001 tagagcatct tgtccggtcc gaaccgcagc gtgccgccgt tgtggttcac atacggcttg
     3061 tcctgcgtga acaggatgcg agcactcttc ggatcagcct tgttcgggtc atcggacgag
     3121 accttgtact cgctgaggac tacatcgccg ttggtctggt aatcgctgta gtagacgtag
     3181 aagagaccat tcgtcttgta gtcgggcgag aaggcaaggc cgagtagacc ctgctccagg
     3241 aagtcggtct tgacgaggct ctgaatgtcg aggaacggat cggggagcaa cttcccatcc
     3301 ttgtcgacga tccggatccg accgatccgc tcgacgacga aaatgcggcc gctgccgtcg
     3361 ttggcgctgg cgatattgat cgggtcggcc aagccgccgg cgactttcac aagctggatc
     3421 ttcgggttgt taggaatcgt gcctcctggc gtggcgacgc cctgaaagtc cggattgtcc
     3481 gggagtgact ggccctgatc ctgggcgcgt gccggggttg gactgctcgg ggcgagcaag
     3541 ccgaaccccg ggatcaatag cgtcaggatg acgacgagtg cgacaagggt gcgagttctc
     3601 acgcgagatc gacctttcgt ttgggcacgc acaggccgcg ttgcgcgatg cctcggctgg
     3661 ctgagcgcgc ggcggaaaac cggacccagc ggtcgaaggt ccacgcatcg cttcgggcga
     3721 ggcggcgtcg acgtggcggc gcggtcgaac ctgttgcgac ggatacctgc cgtgcgcggc
     3781 aattgctttc aaccgtaggg ctgagaagtc aacactggcg tatcgattgg tgaacggaat
     3841 tggaaacgat cggtgaatat gcgtgaacag atgcgcggcg gattctgtag ccgaggatca
     3901 aatccaacac gaacgctggg cagttctcgt ccgtaatctc agcgtgcgta tgcgcttttc
     3961 cgagccgttt gccaccggtg gttctcggcg aagccgcacc tcggagccgt caaagcgaac
     4021 tgcagcaata ttcaccccgg tcgaagacgg cagggacgcg agcagccgat gacttcggga
     4081 tcaaatgcgt cgccttcgag ggagaagtca gatgccgatg acgttcctcg tcgccgaaga
     4141 atccagcgac accgcccatg ggatcgagtt cagcgcgcag atgtcgtggc ccggctgccg
     4201 ggtcatcacg gccggaaccg gagcggaagc gatcgcccga tttgctgagg cgcccgccga
     4261 tgccgtactg ctcgatgtcc agttggagcc atctgacgga ttcgaagtct gccggcagat
     4321 tcgggagatg tcgaatgtcc cgatcgtgat gctctcagcc gatgatgcca ttgtcgacaa
     4381 gattcgcgcg ttcgacctcg gcgcggacga ttacctggtc aagcccttcg atcatctcga
     4441 actcatggcc aggctacggg cgctcctgcg ccgggccgag atgggctccg cgcatgcgat
     4501 tggcgagtat gcctgtggtg atctggcgat agatttcggg ttacacatgg tcagatgcca
     4561 tggtgaggag gtgcggttga cctcaaccga gttccggctg ctcgaggtgc tggtcagaaa
     4621 cgcgggcatg acgatgccgc atcatgtcct gctcgaccgg gtctggggat cagaatgggg
     4681 tggccgtccg ggctacctca aggtgttcgt ccgccgcctg cgccaaaagc tggacgacga
     4741 cgccgagcga ccccgctaca tcgaaaccgt ctggggcacc ggctaccgct tcctcccgta
     4801 ctcggtgtaa cgcctttcac tcggtcgagg tggagcttaa cggcgggtca ggcgctgttg
     4861 gcgggcgagg cgggcttgga gcaggacgac
//
LOCUS       DATAOZ010000362         6555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3304557, whole genome shotgun sequence.
ACCESSION   DATAOZ010000362 DATAOZ010000000
VERSION     DATAOZ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3304557"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VHR64_09245"
     CDS             <1..392
                     /locus_tag="VHR64_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3303053.1"
                     /translation="GIGQQPPAEQVLREHDYALAREQLPVGAFDATWSAGRVMPLDDV
                     IAEALSIAMAVASGDDAVAENPGGLTPREVQVLGLLVDGLSDREIAERLFVSRHTAAN
                     HVGSILSKLGVPSRAAAAAWAVRNGIA"
     gene            597..1094
                     /locus_tag="VHR64_09250"
     CDS             597..1094
                     /locus_tag="VHR64_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303054.1"
                     /translation="MSRKLLHSLSFLVALAVLGILVVGASADAPAPARSSGPVLTAVL
                     ASAAQPGSISVSGEGFTPGGDVYVAIYDTWGAELHETRWTTASIAAYGPSGSLDPALG
                     YHAGGRVSESFAGLCGNAIMVRAFDRQAAVWTNLLDVDSAAITAAFFGLNGSLDPAMG
                     YRAGC"
     gene            complement(1156..1908)
                     /locus_tag="VHR64_09255"
     CDS             complement(1156..1908)
                     /locus_tag="VHR64_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303055.1"
                     /translation="MLFLAIPLFALSWRAIAHLDDLQQAGRVTVVDALVLSATTSLTS
                     LLVIIGLGTPLAFLIARRSFRGKTVLETLIDMPMVMPPAVAGLALLMAFGRRGVLGSW
                     LDDAGIRIGFTTTAVIFAQIFVSSPFYVRSGRAAFSRVDRNMEDAAADLGASPWRVLR
                     SVTLPLAAPGLIAGAVLAWARAVGEFGATIMFAGNLPRETQTLPLAIYGRYEAGDLET
                     SLAMALILLVSSFLVLVSVRGLGGRLRLSAAD"
     gene            complement(1977..2873)
                     /gene="modA"
                     /locus_tag="VHR64_09260"
     CDS             complement(1977..2873)
                     /gene="modA"
                     /locus_tag="VHR64_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3303056.1"
                     /translation="MTGRIAMAPVQIMLAVMMLAGMAIWHQPGFARAQTWSCDVPASA
                     SPAASPRPAATPQPVAFPDEGGKLTIFAAASLTDAFTKMADDLKTAHPNLDITFNFAG
                     SQALVTQIAEGGAQADVLALASPAQMKSAVEEGIPAEGSATFVTNRLTVVVPADNPAG
                     IASVADLAKDGVRLVLANPDVPAGNYARQSLCNAAAAGGPSVDDYAANIVSEEDNVRS
                     VLTKVELGEGDAGIVYVTDALTSGDKVRSIEIPADQNVIASYPIATIGDNPLAAAFVG
                     YVFSPEGQATLATFGFNPVSAT"
     gene            3044..3352
                     /locus_tag="VHR64_09265"
     CDS             3044..3352
                     /locus_tag="VHR64_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEX3303057.1"
                     /translation="MPQTVYAPKIHSGLDPQVVLDRLRRDGWDPIPINDPAGHIYPPH
                     SHATTKLLAILFGSMEVRMAGENYQCLPGDQVVIPGGVEHAALVGPNGCQFFWSEQLR
                     "
     gene            3349..3621
                     /locus_tag="VHR64_09270"
     CDS             3349..3621
                     /locus_tag="VHR64_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX3303058.1"
                     /translation="MSERRVTIRVEGMVQGVNYRQAARREAERLGIAGFARNEDDGSV
                     TVEAEGESNTIAQFIEWCRHGPAPAHVTRVDVVDGPFVGYEGFARR"
     gene            3706..4248
                     /locus_tag="VHR64_09275"
     CDS             3706..4248
                     /locus_tag="VHR64_09275"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3303059.1"
                     /translation="MTQTPTLRERQAPLKAKYESDPESARLTITVRSCTRTADPTRCE
                     ISTEAGGGVSWKVGAHPYAGGVGDLACSGDVLLASLAACQEITLRMVAAAMGITLEEV
                     EVTVHGDMDFRGTMGVDPETPVGFQKIRTEIRVVADAPEDRLQRLAQRAEKYCVVAST
                     LRQSPEMETSFQAEQLASGG"
     gene            4353..5705
                     /locus_tag="VHR64_09280"
     CDS             4353..5705
                     /locus_tag="VHR64_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEX3303060.1"
                     /translation="MSVSSNGHGAESPDGQDHIFGFDTLTVHAGQRPDPTTGARAMPI
                     YQTTSFVFEDTDHAAELFALQRFGNIYSRIMNPTNGAFEERMATLEGGVGALAVASGH
                     AAQFIALATILNPGDQIVSSNALYGGSYTQLGITLRKWGIDVVFVDPDDPENFRRAIT
                     PKTRALYGETIGNPRINVLDIQALADIAHEAGVPLIIDNTFATPYLCRPIDFGADIVV
                     HSATKFIGGHGTSIGGVIVDAGRFPWDNGNFPGLTEPSPGYHGMRFYEQFNIYGFIMK
                     ARVETLRDIGPALSPFNSFLFLLGLETLHVRMDRHVQNAQKIAAYLADHPLVSWVNYP
                     GLTDSPYHALAQKYMPKGAGAIFCFGVKGGREAGKAFIESLDMISHLANVGDAKTLVI
                     HPASTTHQQMSDEEQIAAGLSPDFIRISIGLEDPADIIWDLNQALTKAARIASVSAGA
                     "
     gene            5709..6203
                     /locus_tag="VHR64_09285"
     CDS             5709..6203
                     /locus_tag="VHR64_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX3303061.1"
                     /translation="MAVLTDHVPSASASERLRILNDAKTIAMVGLSSDPMRPSHFAAI
                     YMQSEGYKIVPINPRYAGQKILGETVYASLSDIPFPVDIVDVFRKPEECPGLAEEAIK
                     IGARVFWIQLGIESDEAGRIAREGGLDFVQNRCVKIEHARFHGGMNLVGLATGVISGR
                     RKLA"
     gene            6200..>6555
                     /locus_tag="VHR64_09290"
     CDS             6200..>6555
                     /locus_tag="VHR64_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX3303062.1"
                     /translation="MIEPTDDEIRSLLVTARRIAVVGLSTDESRASYGVSAYMQREGY
                     EILPVNPKHVGQMILGQPVVAKVTDLEGPIDIVNIFRRSIDVPRPVDEAIAAAAGAIW
                     MQLGIRNDEAARKAIDA"
BASE COUNT         1204 a         2139 c         2097 g         1115 t
ORIGIN      
        1 acggcatcgg acagcagcct ccagcggaac aagtcctccg cgagcatgac tacgcattgg
       61 ctcgcgagca gttgcctgtt ggcgcatttg acgccacctg gtcagcgggc cgggtaatgc
      121 cactcgatga cgtcattgcc gaggcgctgt cgatagccat ggcggtcgca tcgggtgatg
      181 acgcagtagc ggagaatcca ggtggcctga cgccacgcga ggtgcaggtt ctgggtctgc
      241 tggtcgacgg gttgtccgac cgggagatcg ctgagcggct cttcgtcagc cggcataccg
      301 ccgccaacca cgtcggcagc atcctcagca agctcggcgt gccctcccgc gccgccgcgg
      361 ccgcctgggc ggttcgcaac ggcatcgcct gactgctccc cgccgcattg aacaaattac
      421 ctacgcctgt cccggcatga tcgaagttca aaatgacgat cctgcctcat gtgcagcgcg
      481 ccacctcgac ctacggtggt tgcggaggga aaggcggacc cgccgatccg gacggcgggc
      541 atcctccgat tccctcctcc gtcttcctca tccgcattca cgaagggaga aatcccatga
      601 gccgcaagct ccttcactcg ctttcgttcc tcgtcgctct ggcagttctc ggcatattgg
      661 tcgtcggtgc ctcggcggac gcacccgctc cggctcgatc gtccggtccg gtcctgaccg
      721 cggttctggc ctcggcagct caacccggct cgatctccgt ctcaggcgaa gggttcaccc
      781 cgggcggcga tgtctacgtc gcgatctacg acacctgggg cgctgagctc catgaaaccc
      841 gctggaccac cgcaagcatc gcggcctacg gaccgagtgg gagcctcgac cccgccctcg
      901 gttatcacgc tggtggccgg gtcagcgaat cgttcgccgg gctttgtggg aatgcgatca
      961 tggttcgggc cttcgatcgg caagcggccg tctggacgaa cctgctcgac gtcgactccg
     1021 cggcgatcac ggcggcattc ttcgggctga acggcagcct cgatccggca atgggatacc
     1081 gcgccggttg ctgacctgca tcccacaatt cacaggaacg gacccgccga agggtatccg
     1141 gcgggtccgt tcttgctaat cagcggcaga taaccgcagg cggccgccga ggccgcggac
     1201 gctgacgagc accaggaagg acgacacgag caggatcagg gccattgcca gcgacgtctc
     1261 gaggtcgccg gcttcgtacc ggccgtagat cgcgagcggc agcgtctggg tctcacgggg
     1321 cagattgccg gcgaacatga tcgtcgcccc gaactcgccg accgcccgcg cccaggccag
     1381 gactgccccc gctatcagcc cgggcgcggc caatggcaac gtgaccgacc gcaacacccg
     1441 ccacggtgac gcgccgagat cggcggccgc atcctccatg ttccggtcga cgcggctgaa
     1501 ggcggcacgc cctgaccgca cgtagaacgg cgacgagacg aagatctggg cgaagatgac
     1561 ggcagtcgtc gtgaagccga tccggatccc ggcatcgtcc aaccagctcc ccaaaacgcc
     1621 ccgccgcccg aacgccatca acagggcgag gcctgccacc gccggcggca tcaccatcgg
     1681 catgtcgatc agcgtctcga gcacagtctt cccccggaac gaccgccggg cgatcaggaa
     1741 ggcgagcggc gtgcccagcc cgatgatcac gagcaggctg gtcagcgagg tcgtcgccga
     1801 gagcacgagc gcgtcgacga ctgtcactcg cccggcctgc tgcaggtcat cgaggtgcgc
     1861 gatcgcccgc cacgagagcg cgaagagcgg gatcgccagg aacagcacca ggacgccgcc
     1921 caggatccat gggacggtgg agccccggtg cgatgggctg tcagcaggtg ccgatgtcag
     1981 gttgcgctca ccgggttgaa tccgaacgta gccagtgtcg cctgtccttc gggcgaaaag
     2041 acgtacccga cgaacgcggc agcaagagga ttgtcgccga ttgtcgcgat cgggtagctg
     2101 gcgatcacgt tttgatcggc cggaatctcg atcgaccgaa ccttgtcacc ggaggtcaga
     2161 gcgtcggtga cgtagacgat gccagcgtcc ccctcgccga gctcgacctt ggtcaggacc
     2221 gagcggacat tgtcctcctc ggaaacgatg ttcgcggcgt agtcatcgac cgacgggccg
     2281 ccggccgctg cggcattgca gagcgactgc cgcgcgtagt tgccggccgg gacatccggg
     2341 ttggcgagga cgagccggac accatccttc gcgagatcag cgaccgaggc gatgccagcc
     2401 ggattgtcgg cggggaccac gacggtcagg cgattcgtga cgaaggtcgc cgatccctca
     2461 gctggaatgc cttcctcgac agcgctcttc atctgcgcgg gactggcgag cgccagaaca
     2521 tctgcctggg cgccgccctc ggcgatctgg gtgacgagcg cctgcgagcc ggcgaagttg
     2581 aaggtgatat cgaggttcgg atgggcggtt ttcagatcgt cggccatctt cgtgaaagca
     2641 tcggtcaggg aggccgccgc gaagatggtc agcttgcctc cctcatccgg gaaggcgacc
     2701 ggttgcggtg tcgcggcagg tctgggggat gccgccgggg aggctgacgc cgggacgtcg
     2761 caactccacg tctgcgcccg ggcgaagccc ggctggtgcc agatcgccat gcccgccagc
     2821 atcatcacgg cgagcatgat ttgcaccgga gccatcgcga tgcgtcccgt catccgtcgc
     2881 tccctctcac ttccgacatc cggcccgata tagtcaatag ttgactattt tccggagtgc
     2941 agcctatggc agggccagaa tgctgtcaag caccggccgc cgcgaacatc atgctggtat
     3001 cgtcaccagt gacggtaatc gattgcagtc atggagccgg acgatgccgc agaccgtcta
     3061 cgcaccgaaa atccattccg gtctcgatcc ccaggtcgtg ctcgatcggc tgcggcgcga
     3121 tggttgggac ccgataccga tcaacgatcc ggcggggcac atctacccgc cgcactccca
     3181 cgctacaacc aagctgctgg cgatcctctt cggcagcatg gaggtgcgga tggccggcga
     3241 aaactaccag tgcctgcctg gcgatcaggt cgtcatcccg ggcggtgtcg agcatgcggc
     3301 gctggtcgga ccgaacggtt gccagttctt ctggagcgag cagctacgat gagcgagcga
     3361 cgggtgacga tccgggtcga gggtatggtc cagggggtca actatcggca agcagcgcgg
     3421 cgggaagcgg aacgcctggg cattgcggga ttcgcccgga atgaggatga cggcagcgtc
     3481 acggtcgagg cggaagggga atccaacacg atcgcgcagt tcatcgagtg gtgccgacac
     3541 ggacccgcgc cggctcacgt caccagggtc gacgtcgtcg atggcccgtt cgtcggctac
     3601 gagggttttg ctcgccgctg attgcgcctt gttttggctt tgccagtggg ccagaattga
     3661 attgttgagc aggaactcca gttttgcgac aggtggagca gtgacatgac tcagacgccg
     3721 acactgcggg aacgacaagc gccgttgaag gccaaatatg aatcagaccc cgaatccgcc
     3781 aggctgacca tcacggtcag gagctgtacc cggacggccg atccgacgcg ctgcgaaatc
     3841 tcgaccgaag caggcggcgg cgtatcgtgg aaggtcggcg cgcatccata tgccggtggc
     3901 gtgggcgatc tggcttgttc gggggacgtg ctcctcgcct cgctggcggc ttgccaggag
     3961 atcacgctcc gcatggtcgc cgcggcgatg ggcatcacgc tcgaagaggt cgaagtcacg
     4021 gtccacggcg acatggattt ccgcggcacg atgggcgtcg atccggagac tccggtcggc
     4081 ttccagaaga tccgaaccga gatccgggtt gtcgccgacg cgccggagga tcgcctccag
     4141 cgcctcgccc agcgtgctga gaagtactgc gtcgtcgcct cgaccctgcg ccaatcaccc
     4201 gaaatggaga cgtcgttcca ggccgagcag ctggccagcg gaggatgatg tcctgggtcg
     4261 gataccggca atacagcatt cgctcgacaa ggttgcagca gaaacgtagg ctgaccgcat
     4321 acggtcattg actgacaagg agccccgcag atatgagcgt gtcttcgaac ggccacggcg
     4381 ccgagtcgcc cgatggccag gaccacatct tcggttttga caccctgacg gtccacgccg
     4441 gtcaacgacc ggacccgacc accggcgcgc gagcgatgcc gatctaccag acgacctcgt
     4501 tcgtcttcga agacacggac cacgccgccg agctcttcgc acttcagcgt ttcggcaaca
     4561 tctactcccg gatcatgaac ccaaccaacg gcgccttcga ggagcggatg gctaccctgg
     4621 agggcggcgt cggtgcgctc gcggtcgcct caggtcacgc cgcgcaattc attgcgctgg
     4681 ccacgatcct caaccccggt gatcagatcg tctcgtcgaa cgcgctttac ggcggcagct
     4741 acacgcaact cggcatcacg ctccgcaagt ggggcatcga tgtcgtcttt gtcgatcccg
     4801 atgatccgga gaacttccgc cgcgcgatca cgccgaagac ccgcgccctc tacggcgaga
     4861 cgatcggaaa tccgcggatc aatgttctcg acatccaggc gctggccgac attgcccatg
     4921 aggcaggcgt gccgctgatc atcgacaaca cattcgccac gccctacctc tgccggccga
     4981 tcgatttcgg cgccgatatc gttgtccact ccgcgaccaa gttcatcggc ggacacggga
     5041 cctcgatcgg cggcgtgatc gtcgatgccg gccgcttccc gtgggacaac ggcaacttcc
     5101 cgggtctgac cgagccgtca cccggatacc acgggatgcg cttctacgaa cagttcaaca
     5161 tctacggctt catcatgaag gcgcgggtcg agaccctgcg tgacatcggt ccggccctca
     5221 gcccgttcaa ctccttcctc ttcttgctgg gtctggagac gctccatgtg cgcatggatc
     5281 gccatgtcca gaatgcgcag aagatcgccg cgtacctggc cgatcatccg ctggtgagct
     5341 gggtgaatta cccgggtctg accgattccc cgtaccacgc gctggcgcag aagtacatgc
     5401 ccaagggagc tggggccatc ttctgcttcg gggtcaaggg cggacgcgag gccggcaagg
     5461 catttatcga gagcctcgac atgatctcgc acctcgccaa tgtcggtgat gccaagacac
     5521 tggtcatcca ccctgccagc accacgcatc aacagatgtc cgacgaggaa cagatcgcag
     5581 ctgggctcag cccggatttc atccggatct cgatcggcct cgaggacccg gccgacatca
     5641 tctgggatct caatcaggcg cttacgaagg cggcgcgaat cgcctccgtc agcgcgggag
     5701 cttgaaccat ggccgtcttg actgatcatg tcccatcggc gtccgcgtcg gagcgcctgc
     5761 gcatcctgaa tgacgcaaag acgatcgcga tggtcgggct ctcgtccgac ccgatgcgac
     5821 cgagccactt cgccgcgatc tatatgcagt ccgagggcta caagatcgtc ccgatcaatc
     5881 cccgctacgc cgggcagaag atcctcggcg agacggttta cgcctcgctg agcgacattc
     5941 ccttcccggt cgatatcgtc gatgtcttcc gcaagccgga ggagtgcccg gggttggcgg
     6001 aggaagcgat caagatcggc gccagggtct tctggatcca gctcggcatc gagagcgacg
     6061 aggccgggcg gatcgctcgg gaaggcggac tcgacttcgt ccagaaccgc tgcgtcaaga
     6121 tcgagcacgc ccgcttccac ggcgggatga acctcgtcgg gctggccacc ggggtgatca
     6181 gcggtcggcg gaagctggcg tgatcgagcc gaccgacgac gagatccgct ccctgctcgt
     6241 gacagctcgg cgcattgccg tggtcgggct ctcgaccgac gaatcgcgcg cgagctacgg
     6301 cgtttcagcc tacatgcagc gcgaggggta tgagatcctg ccggtcaacc cgaagcatgt
     6361 gggccagatg atcctcggcc aaccggtggt ggccaaagtg accgatctcg aaggcccgat
     6421 cgacattgtc aacatcttcc ggcgctcaat cgatgtgccg cgtccggtcg atgaggcgat
     6481 cgcggccgcc gccggagcaa tctggatgca gctcgggatc cgcaatgacg aggcggcgcg
     6541 gaaggcgatc gatgc
//
LOCUS       DATAOZ010000363         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8305164, whole genome shotgun sequence.
ACCESSION   DATAOZ010000363 DATAOZ010000000
VERSION     DATAOZ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8305164"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VHR64_09295"
     CDS             <1..372
                     /locus_tag="VHR64_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303063.1"
                     /translation="GIRVGARTGRASVITALCFLPCLFIAPLAAAVPPYATGAVLVLV
                     GLSMFQMVGALDFRTLEDSLPAFVTIVLIPLTLSITQGILWGFVLHLVLYVVNGRHRE
                     LNPTLWILAIISIGLLGLENR"
     gene            369..602
                     /locus_tag="VHR64_09300"
     CDS             369..602
                     /locus_tag="VHR64_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303064.1"
                     /translation="MNVDLHQTDPTVLWRLTRGRSTAHATIFPGPVTTTVTWYFDGVM
                     DRAENYDSIDLGMARAEQIRGILLRDGWTEPGT"
     gene            658..>1614
                     /gene="ispG"
                     /locus_tag="VHR64_09305"
                     /gene_synonym="gcpE"
     CDS             658..>1614
                     /gene="ispG"
                     /locus_tag="VHR64_09305"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX3303065.1"
                     /translation="MPTVTRLHKTVGVRVGNVQVGGGAPVVVQSMTMTDTANAKATAE
                     QCIELAQAGSEMVRVTVNLPEAAAAVPEIKQRMLDAGCTAPLIGDFHYNGHLLLTRFP
                     DCARALDKYRINPGNVGTGRRRDEQFATICKIAIDQNKPVRIGVNGGSLNQELVTAKM
                     QENTDRTLGRTSEEILNECMVISAVQSTALALETGLRKDQIIISCKVSRPRDLISIYR
                     DLAKQSDQPLHLGLTEAGMGMKGLMWSASAMGILLNEGIGDTIRVSLTPRPGGDRREE
                     VYAACELLQALGIRSFAPSVTACPGCGRTTSSTFQELAERTQD"
BASE COUNT          293 a          538 c          498 g          285 t
ORIGIN      
        1 ggcatccgcg tcggcgcccg caccggtcgg gcgtcggtga tcaccgccct ctgcttcctt
       61 ccgtgcctgt tcatcgcccc gctcgccgct gccgtgccgc cgtatgcgac aggcgcggtg
      121 ctggtgctgg tcggcctctc catgtttcaa atggtcggcg cgctcgactt cagaacgctc
      181 gaagattccc tgccggcgtt cgtcacgatc gttctgattc cgctgaccct gtcaattacg
      241 cagggcatcc tctggggctt cgtcctgcac ctggtcctgt acgtggtgaa tggccgccac
      301 cgcgaactca atccaacgct gtggattctt gcgatcattt ccatcggcct gcttggcctc
      361 gagaatcgat gaacgtggac ctccatcaga ctgacccaac cgtgctctgg cgcctgacgc
      421 gtggccggtc taccgcgcac gcgacgatct tcccggggcc cgtcaccacc accgtcacct
      481 ggtacttcga tggcgtgatg gaccgcgccg agaactacga ctcgatcgac ctgggtatgg
      541 cgcgggccga acagatccgc ggaatcctct tacgcgatgg atggaccgaa cccggaacct
      601 gaacacgaac tgagaaccga gaacccggaa gcgtgaacgg ctgctatcct taagtcggtg
      661 cccaccgtca caagactgca caagaccgtt ggcgtccgcg tcggcaacgt ccaggtgggc
      721 gggggcgcgc ccgtcgttgt ccagtcgatg acgatgacgg atacggcgaa tgcgaaggca
      781 actgcggagc agtgcatcga actggcccag gccggttcgg agatggtgcg cgtcaccgtc
      841 aatctgccgg aagccgcggc cgcggtcccc gaaatcaagc agcgcatgct cgatgccggc
      901 tgcacggcgc cgctcatcgg cgacttccac tacaacggtc acctcctcct cacccgattt
      961 ccggactgtg cccgcgcgct cgacaagtac cggatcaatc ctgggaatgt gggcaccggc
     1021 cgccgacgcg acgagcagtt cgccacgatc tgcaagatcg cgatcgatca gaacaagccg
     1081 gtccgcatcg gcgtcaacgg cggttctctc aatcaggagc tcgtcaccgc caagatgcag
     1141 gagaacaccg accgcacgct cggcaggacc tcagaggaga tcctgaacga gtgcatggtg
     1201 atctcggccg tccagtcgac tgcactggcg ctcgaaacgg gccttcgcaa ggaccagatc
     1261 atcatctcgt gcaaagtgtc gcggccgcgc gacctgatct ccatctatcg cgacctggcg
     1321 aagcagtccg accagccgct gcatctcggc ttgaccgagg ccggcatggg catgaaggga
     1381 ctgatgtggt cggcgtcagc gatgggcatc ctgctcaacg aggggatcgg cgacaccatt
     1441 cgcgtatcgc tgacaccgcg ccccggcgga gatcgccgcg aagaggtcta tgcggcgtgt
     1501 gagctgctgc aggcacttgg gatccggtcg ttcgcgccga gcgtgacggc ctgtcccggc
     1561 tgcggacgca cgacgagcag tacgttccag gagctcgccg agcgcacgca ggat
//
LOCUS       DATAOZ010000364         6462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4355590, whole genome shotgun sequence.
ACCESSION   DATAOZ010000364 DATAOZ010000000
VERSION     DATAOZ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4355590"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VHR64_09310"
     CDS             <1..422
                     /locus_tag="VHR64_09310"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX3303066.1"
                     /translation="SDELGDLLIQVALHAQMANENGDFDAADVFDAISSKLIRRHPHV
                     FGDVVAKDPAAVLRTWDAVKQSEPGKEDLAQRAPIDKLPASMPASLKIAEIETPDGSS
                     ADADTLSREIAERMVALARAGVDIDRAVERAYRILER"
     gene            459..2300
                     /gene="aspS"
                     /locus_tag="VHR64_09315"
     CDS             459..2300
                     /gene="aspS"
                     /locus_tag="VHR64_09315"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX3303067.1"
                     /translation="MPVSYDVDPSALGRLTCGSLTVDDEGQSVELNGWVNRRRDLGGL
                     IFIDLRDRFGITQIVFNPEIDAPAHEIASTVRNEYVLKVSGIVRRRTPETINPRLKTG
                     TIEIEARGIEILNTSLTPPFYINEEVEVDESLRLEHRYLDLRRPSMQQNLILRHQIVK
                     HMRDYLTDRAFIEVETPMLIKSTPEGARDFLVPSSSFPGLMYALPQSPQQMKQLLMVA
                     GVDRYFQIARCFRDEAQRADRQPEFTQLDIEMSFVSPDDVTGLIENLFIESSERFSGK
                     TIVQKPFPRISYHDAIEKYGTDRPDLRFGLDFQNVTAKFGSTEFRAFADVVANGGQIK
                     ALVAPDSADYTRREIDELTEIAKRAGAKGLATVAMQEGGVKSPIAKFLSESEIAALTT
                     DIGAKTGDLVLIVADTPAVVAKALSTLRDELGQRHGLADPNALAYCWVVDFPLLEWDE
                     ENQRWDSPHNPFCGFVEEDRQKLDTDPGNIRSKQYDLVLNGNEVGGGSVRNHRREDQA
                     KIFTLMGHSPEAQQDRFGAILTALEYGAPPHGGIAMGVDRYVMLLADETNIRDVIAFP
                     KNQRGVDLMFDAPSTVDEQQLCDVGLELLPDVRDQVAAEAEIVKQAR"
     gene            2530..3090
                     /locus_tag="VHR64_09320"
     CDS             2530..3090
                     /locus_tag="VHR64_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3303068.1"
                     /translation="MFRIDPAEVDRFERRTRPATVVPQLAQTPVDPSPENRRSAEEWE
                     LFRRLVSALSAIRALTGLGFVYLAQDDLKRTLAVQWEVLNVVQRVCARSLVDSLVNDD
                     TTTDGGRWMSIVPSSTARIGEIALTRREVEVLHLMAMGESNRDIADSLYISIPTVKRH
                     VTNILGKLGVSSRYEAASFARSHGFA"
     gene            3270..4430
                     /locus_tag="VHR64_09325"
     CDS             3270..4430
                     /locus_tag="VHR64_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX3303069.1"
                     /translation="MTTPSISRRTMVKQGAAMAGLSMLRISVPAHAFQGDSGGEVIPW
                     LDKLEPNPVPEIVPVQLDWAKLDSWITPQDQFFVVKHFNLPELNEKDWSLAISGQVAN
                     PLTFTLEDIKARARREVEFTIECSGNTGLPFFNGGVGNAKWAGTALAPLLREAGVLES
                     GIEVVFWGADSGEHKYGEMTVTENMARSMSLADAMEGDNILAYEMNGEPLPPLHGYPL
                     RLIAPNWYGIANTKWLTRIEVLDHRFQGFFMAQEYVSISQQERDGKEVWVFTSVGRKR
                     LKSAPAKVTKSGDKYSIMGAAWGAQIAKVDVRIDDGDWQPATLSDGEGSEFAWVFWTF
                     DWKSPAAGEHAITSRATSITGEVQPTPDDPFLSSKTTYYETNGQITRKVKIT"
     gene            complement(4533..5534)
                     /locus_tag="VHR64_09330"
     CDS             complement(4533..5534)
                     /locus_tag="VHR64_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX3303070.1"
                     /translation="MSRRWSRRSVVQAAFGGTAVVAGLARRSAHAAIDPRQLPGKIAF
                     TRNGSIWVWSGGTANELVTADNISDPRWSPDGNMLLYVRMQNSYSDLYTYDLATGAET
                     QITANQPYDEVGSFDYTINSSWALDPDWSRSGLIGFISDTYGSAGYLSLWLLASPADN
                     PYLALQTVQEDDISGLALAPHDAIAAYTVRVRMGDGTFSTYVALRDLNNGFTGPVAQS
                     SGDIFDPAISPDSAWVAVTIRDDEGVTDIWLVERATGERRRATRGENAMAPRWSDDGN
                     WFAYLRMQDYQFEIWAGQFSRGRLRDQARIYQADGIDSQSGLSWWMPGEPATATPIP"
     gene            complement(5531..>6462)
                     /gene="murE"
                     /locus_tag="VHR64_09335"
     CDS             complement(5531..>6462)
                     /gene="murE"
                     /locus_tag="VHR64_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HEX3303071.1"
                     /translation="LDHTRFEIATVTNITQEHLEHHKSIEAYWRAKAKLFERASTIGG
                     DAVVNLDDAGARSVVRYCSGCRRILTYSLSEESADLLAEIVESGLSGSRGLLRTRKAT
                     YPFEIPLVGAYNVSNSLAALGTAMAAGVEPGDAVDALRTVPPVPGRMTAVDAGQPFGV
                     IVDYAHTPESLDKVLRLLRSLRPDGRLIVVFGSAGERDPIKRPIQGRVAAELADIAII
                     TNEDPRFEDAESIISEIAAGAIEGGAVEGEDVHCVVERRDAVALAMDIARPGDTILLA
                     GKGHERSIIWNGIKHPWDEAAVAYDELAKRGWA"
BASE COUNT         1199 a         2107 c         2011 g         1145 t
ORIGIN      
        1 tcagcgacga gctgggcgac ctgctgatcc aggtcgcgtt gcatgcccag atggccaatg
       61 aaaacggcga tttcgacgcc gcagatgtct tcgacgcgat ctcatcgaag ctgattcgcc
      121 gccacccgca cgtcttcgga gatgtcgtcg cgaaggaccc ggccgccgtg ctgcggacct
      181 gggatgccgt caagcagtcg gagcccggca aggaagatct ggcccagcgc gcgccgatcg
      241 ataagctccc ggcctccatg ccggcctcgc tcaagatcgc cgagatcgag acgccggacg
      301 gttcatccgc cgatgccgat acgctcagcc gggaaatagc cgagcggatg gtcgcccttg
      361 cccgggccgg cgtcgatatc gaccgcgcag tcgagcgggc atatcgcata cttgagcgtt
      421 gatcgtttct gatcgctgaa ccaaaggaaa agccacccgt gcctgtttcg tacgatgtcg
      481 atccgtctgc ccttggacgt ttgacctgcg gctccctcac cgtcgacgac gagggccagt
      541 ccgtcgagct caacggctgg gtgaatcgcc gccgcgacct cggtggcctg atcttcatcg
      601 atttgcgcga ccggtttggc atcacccaga tcgtcttcaa cccggagatc gacgcgcccg
      661 cgcacgagat cgccagcacc gtccgtaacg agtacgtgct gaaggtcagc ggcattgtcc
      721 gccgacgcac gcccgagacg atcaacccgc gcctcaagac cggcacgatc gagatcgagg
      781 cgcgcggcat cgagattctg aacacctcgc tgacgccccc cttctacatc aacgaggagg
      841 tcgaggtcga cgagagcctg cgtctcgagc accgctatct cgacctgcgc cgcccctcga
      901 tgcagcagaa cctgatcctg cgccatcaga tcgtcaagca catgcgcgac tacctgacgg
      961 accgcgcctt catcgaggtc gagacgccga tgctaatcaa gagcaccccg gaaggcgcgc
     1021 gcgatttcct ggtgccgtcc agcagcttcc cgggcctgat gtatgccctg ccgcagtcac
     1081 cgcagcagat gaagcagctc ctgatggtgg ccggcgtcga ccgctacttc cagatcgccc
     1141 gctgcttccg cgacgaggcc cagcgcgcgg accgccaacc ggagttcacc cagctcgaca
     1201 tcgagatgtc gttcgtctcg ccagacgacg tcacggggct gattgagaac ctgttcatcg
     1261 agtcgtccga gcgcttctcg gggaagacca tcgtccagaa gccgttcccg cgcatctcgt
     1321 accacgacgc catcgagaaa tacggcacag accggcccga tcttcgcttc ggcctcgact
     1381 tccagaacgt gaccgccaaa ttcggatcga ccgagttccg cgcgtttgcg gacgtcgtgg
     1441 cgaacggcgg gcagatcaag gccctggtcg ctccggattc ggccgattac acccgtcgcg
     1501 agatcgacga gctgaccgag atcgccaagc gcgcgggggc gaagggtctg gcgaccgtgg
     1561 cgatgcagga gggcggcgtc aagtcgccaa tcgccaagtt cctctccgag tcagaaatcg
     1621 ccgcgctgac gaccgacatc ggcgcgaaga ccggcgacct cgtccttatc gtggccgaca
     1681 cgccggctgt cgttgccaag gcgctctcga cattgcgcga cgaactcggc cagcgacacg
     1741 gcctggccga tccgaacgcg ctggcctatt gctgggtcgt cgatttcccg ctgctcgagt
     1801 gggacgagga aaaccagcgc tgggactcgc cgcacaaccc gttctgcggc ttcgtcgaag
     1861 aggaccggca gaagctcgac accgatcctg gcaacatccg ctcgaagcag tacgaccttg
     1921 tcctgaacgg caacgaggtc ggcggcggct cggtccggaa ccaccggcgc gaggaccagg
     1981 caaagatctt cacgttgatg ggccattcgc cggaagcgca gcaagaccgc ttcggcgcga
     2041 tcctgaccgc gctcgagtac ggtgcgccac cgcacggtgg catcgcgatg ggcgtcgatc
     2101 gctacgtgat gctgctggcc gacgagacca acatccgcga cgtcatcgcc ttcccgaaga
     2161 accagcgcgg cgtcgacctg atgttcgacg cgccctcaac ggtcgacgag cagcagctct
     2221 gcgacgtcgg gctcgagctc ctgcccgacg tccgcgacca ggtcgcagcc gaggcggaga
     2281 tcgtcaagca ggctcgctag gcagggcgag accacaatac gccggggtat tgcgctttct
     2341 accctgatcc cccattctgg ggcgaccatg gacggacaat ctgcccgcga ccctgcaccc
     2401 ggcgtgtccg gtgctgcgga cacctggccc ctttcagccc gagacgccgc cgctgtcctc
     2461 ggcatcagcg agcgcacggt ccggcgtgcc atcggccgcg gtgacctgtt ggctgcgaag
     2521 catggcggcg tgttccggat cgatcccgcc gaggtcgatc ggttcgagag acgaacccgg
     2581 ccggcgacag tcgtacccca gctcgcccag actccggttg atccatcccc ggagaaccgg
     2641 cgctctgctg aggaatggga actgttccgc cgattagtct cggccctgtc ggccatccgt
     2701 gcgttgaccg gtctcggatt cgtctacctg gcgcaagacg atctcaagcg aaccctggcg
     2761 gtgcagtggg aagtgctcaa tgtggtccaa cgggtatgcg cccgctcatt ggtcgacagt
     2821 ctcgtcaacg atgacaccac gaccgacggt ggccggtgga tgtcgatcgt cccgagctcg
     2881 accgcacgca ttggcgagat tgcgctgact cgacgcgagg tcgaagtgct tcacctgatg
     2941 gcaatgggcg aatccaaccg cgatattgcc gattccctgt acatcagcat ccccaccgtg
     3001 aagcgacacg tcacgaatat cctcggcaag ctcggggtct cgtcccggta cgaggccgca
     3061 tcgttcgcgc ggtcccacgg gttcgcatag ccaatttccg tcccgatcaa attacgaccc
     3121 ccggttcgat gtgcttcgca tgcgaaacga ttcacactgt tgctgcccat gtgggacatt
     3181 cgaagtgtct ggtagtcatc gaaggcgcgt gttgcgggtt ccgtgattcg tcgcccctga
     3241 aggcttccag cggagcaagg agcacggcaa tgacgacccc ctcaatctcc cgaaggacca
     3301 tggtgaagca gggcgcggca atggctggct tgagcatgct gcgaatctca gtaccagcgc
     3361 atgcctttca gggcgactct ggcggggaag tcattccctg gctggacaag ctggagccca
     3421 acccggttcc agagattgtc cccgtccaac ttgattgggc gaaactggat tcctggatca
     3481 caccacagga ccagttcttc gtcgtcaagc atttcaacct gccggagctg aatgagaagg
     3541 attggtcgtt ggcgatcagc gggcaagtag caaacccgtt gaccttcacg ctggaagaca
     3601 tcaaggcacg cgcgcgccgg gaggtcgagt tcaccatcga gtgttccggc aacacggggc
     3661 tgcctttttt caacggcgga gttggtaacg cgaagtgggc cggaacggcg ctggcgccgc
     3721 tgctcaggga agcgggggtg ctggagtccg gcatcgaggt cgtcttctgg ggagcagact
     3781 ccggcgagca caagtacggc gaaatgacgg tcaccgagaa catggcgcgc agcatgtccc
     3841 tggcagacgc aatggaaggc gacaatatcc ttgcctatga gatgaatggt gagccgctcc
     3901 ctccgctcca tggatatccg ctccggctga tcgcgccgaa ctggtacggc atagcgaaca
     3961 ccaagtggct gactcgcatt gaggtgctgg atcaccgctt ccagggcttc tttatggcgc
     4021 aggagtacgt ctcgatctcg cagcaagaac gcgacggcaa agaggtttgg gtcttcacct
     4081 ctgtgggccg caagcgactc aagtcggccc cggccaaggt gacgaagtcc ggcgacaagt
     4141 acagcatcat gggagcagcc tggggggcgc agatcgccaa ggtagacgtc cgcattgacg
     4201 atggagattg gcagccggca acgctctcag acggcgaagg ctccgagttc gcgtgggtct
     4261 tctggacctt cgactggaag tcgcccgcag ccggcgaaca cgccatcaca tcgagggcca
     4321 cgtcaatcac cggggaggtt cagccaacgc cggatgaccc gttcctgagc agcaaaacga
     4381 cctactacga gaccaacggc cagatcactc ggaaggtcaa gatcacgtaa cccaaggcgt
     4441 agcgcggggc cgccgtgccc cgctttggtc cccaatgcgc cagctggcgt gtggatacat
     4501 tcccattatg gtcagggcca ttgggggctg ctctacggaa tcggcgtcgc agtcgctggt
     4561 tcgccgggca tccaccagga gaggccgctc tgggagtcga tcccgtcggc ctggtagatc
     4621 ctggcctggt cccggaggcg gcctctcgaa aactggccgg cccagatctc gaactggtag
     4681 tcctgcatcc gcaggtacgc gaaccagttg ccgtcgtccg accagcgggg agccatggcg
     4741 ttctcgccac gggttgcccg tctgcgttcg cccgtcgctc gctccaccag ccagatatcg
     4801 gtgacgccct catcgtccct gatcgtgacc gcgacccacg cgctgtcggg actgatcgca
     4861 gggtcgaaga tatcgccgct ggattgggcg accggaccgg tgaaaccgtt gttgaggtcg
     4921 cgcagggcga catacgtgct gaatgtgccg tcgcccatcc ggacccgcac ggtgtacgcg
     4981 gcgatcgcgt cgtgcggcgc gagcgccagg cctgagatgt catcctcctg caccgtctgg
     5041 agcgcgaggt acggattgtc agctggggat gcgagcagcc agagcgaaag gtagccggcc
     5101 gatccgtagg tgtccgagat gaagccaatc agccctgatc gcgaccagtc tgggtcgagt
     5161 gcccacgagc tgttgatcgt gtaatcgaag ctaccgacct cgtcgtacgg ctgatttgcg
     5221 gtgatctgcg tctcggcgcc ggttgccaga tcgtaggtgt agaggtccga gtagctgttc
     5281 tgcatccgga cgtagagcag catgtttcca tcggggctcc agcgcgggtc gctgatgttg
     5341 tcagcggtga cgagctcgtt cgccgtcccg ccgctccaga cccagatgct tccgttgcgc
     5401 gtgaatgcga tcttgcccgg gagttgccgc ggatcgattg ccgcgtgcgc tgatcgccgc
     5461 gcaagtccgg cgacaacagc agtgccaccg aaggctgcct ggacgacgga gcggcgcgac
     5521 cagcggcgac tcatgcccaa ccccgcttgg ccaactcgtc ataggcgacg gccgcttcgt
     5581 cccacgggtg cttgatgccg ttccaaatga tgctccgctc gtgacccttg ccggccagca
     5641 gaatcgtgtc gcccggccga gcaatgtcca tcgcgagcgc aacggcatcc cgccgctcga
     5701 cgacacaatg gacatcttca ccttcgactg cgccgccttc gattgcgccc gcggcaattt
     5761 cgctgatgat cgactccgca tcctcgaagc gcgggtcctc gttcgtgatg atcgcgatgt
     5821 ccgcaagctc ggcagcgacc cgcccctgga tcggccgctt gatcggatcg cgctcgccgg
     5881 cgctcccgaa cacgacgatc agccgaccgt cgggccgcag ggagcgcagc aatcgcagca
     5941 ccttgtcgag cgattccggc gtatgcgcgt aatcgacgat cacaccgaag ggctgcccgg
     6001 catcgaccgc ggtcattcgg ccggggaccg gtggtaccgt cctgagcgcg tccacggcgt
     6061 cgccaggctc cactccggca gccatcgccg ttcctagtgc ggcgagcgag ttggacacgt
     6121 tgtaggcgcc gacaagtggg atttcaaagg gatatgtcgc tttacgggta cgcagcaacc
     6181 ctctgctgcc actcaacccg ctctccacga tctcggccag cagatcggcc gattcctccg
     6241 acagcgaata ggtcaggatc cgccggcagc cactgcagta ccgcacgacg gaccgcgcgc
     6301 cggcgtcatc gagattgacg accgcatctc caccgatcgt gctggcgcgc tcgaacagct
     6361 tcgccttggc acgccagtac gcctcgatcg acttgtgatg ctcaaggtgc tcctgggtga
     6421 tgttcgtcac tgtcgcaatc tcgaagcggg tgtgatcgag ac
//
LOCUS       DATAOZ010000365         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6198857, whole genome shotgun sequence.
ACCESSION   DATAOZ010000365 DATAOZ010000000
VERSION     DATAOZ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6198857"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1684)
                     /locus_tag="VHR64_09340"
     CDS             complement(41..1684)
                     /locus_tag="VHR64_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HEX3303072.1"
                     /translation="MTSIVPARFQPALPVDHFIRADAPSAEAVEMDVLFVGAGPAGLA
                     GAIELARLVEADDSIGHIEIGVIEKAGSLGEHNLSGAVVNPRVFRELFPDVPVDKLPF
                     REKVKGEAVYLLTEGKGIRIPTPPTMRNHGNYSASVCEIVRWMGERAEAAGINVFTGF
                     PVDSLLVEDQRVIGVRTTPSGLNRDGSESAEYQEPTDLTAKVTVIAEGTRGSLAQAWQ
                     EWQNVGSANPQIFALGVKEVWQVKQPLERVIHTLGWPLPTDAFGGSFIYPQGPNEVAI
                     GLVVGLDYQASNLDVHELLQRMKLHPLVKPILEGGSLLEWGAKTIPEGGWYALPERRH
                     SDGVMIVGDAAGFVDVPSLKGIHYAMQSGIYAARTAFEALKKGDTSAAALAGYDTLVN
                     ESYIAADLQRTRNVRLAFKSGFYLGGVKAGLATITGGRFPGGKITMHADAEQPREVEL
                     EAQFVPDNVMTFSKLDAVFKSGNATRDTIPNHLVVGQDISPELADFYSHVCPAGVYER
                     EGDELRINAPNCVDCKATDVLGPRWTPREGGSGPAYRGM"
     gene            complement(1786..>2127)
                     /locus_tag="VHR64_09345"
     CDS             complement(1786..>2127)
                     /locus_tag="VHR64_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX3303073.1"
                     /translation="QEAGNFALVEDLAAAFGNAAVGATRAVTDDGWRDPADQIGQTGR
                     VVSPSLYVGVGVSGAIQHLAGMRSSNVIVAINKDKDAPIFKVADYGIVGDLFEVVPKL
                     TEEVKAAKAKS"
BASE COUNT          362 a          753 c          643 g          369 t
ORIGIN      
        1 gggcagattg ccatctgccc ctacagctgt gggaccagcc ctacatcccc cggtaggccg
       61 gaccgctgcc gccctcgcgc ggggtccagc gcggccccag cacgtcggtc gccttgcaat
      121 cgacgcagtt cggcgcgttg atccgcaact cgtcgccctc ccgctcgtag acaccggccg
      181 ggcagacatg gctgtagaag tcggccaact cgggcgagat gtcctgcccg acgaccaggt
      241 ggttcgggat cgtgtcccgc gtcgcattgc cggacttgaa gacggcatcc agcttcgaga
      301 aggtcatgac gttgtccggc acgaactgcg cttccagctc gacctcgcgc ggctgctcgg
      361 catcggcatg catcgtgatc ttgccgccgg gaaaacgacc gccagtgatt gtcgccaacc
      421 cagccttgac accaccgagg tagaagccgc tcttgaacgc cagccgcaca ttgcgcgtcc
      481 gctgcaggtc cgcggcgatg tagctctcgt tgaccagcgt gtcgtacccg gccagcgccg
      541 ccgccgaggt gtcacccttc ttcagcgcct cgaaagcggt ccgcgccgcg taaatgcccg
      601 actgcatcgc gtaatggata cccttgagcg acggcacgtc cacgaacccg gccgcatcgc
      661 cgacgatcat tacgccgtca ctatggcgcc gctccggcag ggcataccag ccgccttcgg
      721 ggatcgtctt cgcgccccat tcgagcagtg agccgccctc gaggattggt ttgactagcg
      781 ggtgcagttt catccgctgg agtagttcgt gcacgtcgag attcgacgcc tgatagtcga
      841 gaccgaccac gagcccgatc gcaacctcgt tcggtccttg cggatagatg aagctgccac
      901 cgaaggcatc ggtcggcagc ggccagccga gcgtgtggat gacccgctcc agcggctgct
      961 tgacctgcca gacctccttg acgccgagcg cgaagatctg cggattggcc gagccgacgt
     1021 tctgccattc ctgccaggcc tgcgccaggc tgccgcgcgt cccttcggcg atgaccgtca
     1081 ccttcgcggt caagtcagtc ggctcctggt actcggcgct ctcggatccg tcgcgattca
     1141 ggcccgacgg cgtcgttcgt accccgatca cgcgctgatc ctcgaccagc aaggagtcca
     1201 ccgggaatcc cgtaaagacg ttgattccgg ccgcttccgc ccgctcgccc atccagcgca
     1261 cgatctcgca gaccgaggcc gagtagttgc cgtgattgcg catcgtcggc ggcgtgggaa
     1321 tgcggatgcc cttgccctcg gtcaggagat agaccgcttc gcccttgacc ttctcccgga
     1381 acggcagctt gtcgaccggc acgtccggga acagctcgcg gaagacccgg ggattgacca
     1441 ccgcgcccga gagattgtgc tcgccaaggc tgccggcctt ctcgatcacc ccgatctcga
     1501 tgtgcccgat cgagtcgtcc gcctctacca gacgcgccaa ctcgatcgcc ccggccaatc
     1561 cggcgggccc agccccgacg aagagcacgt ccatctcaac cgcctcggcc gacggcgcat
     1621 cggcgcgaat gaagtggtca acaggcagcg ctggctgaaa gcgcgcggga acaatggacg
     1681 tcacagcaac tcctcgtcga ggcggtcatc ggtcaacaac ggggaaaggt tggcagatca
     1741 agcccgattg ttgcaacgga acggcgcgta gtggcggggt gtcgtttaac tcttggcctt
     1801 ggccgccttg acctcttcgg tcaacttcgg cacgacctcg aacaggtcgc ccacgatccc
     1861 gtaatcggcc accttgaaga tcggcgcgtc cttgtccttg ttgatcgcca cgatcacgtt
     1921 cgaggagcgc atcccggcca ggtgctggat cgcccccgac acgccgacgc cgacatagag
     1981 cgacgggctc accacgcgac cggtctggcc gatctggtcg gccggatcgc gccagccgtc
     2041 atcggtcacc gcccgggtcg caccgaccgc tgcgttcccg aacgccgcgg ccaaatcctc
     2101 gaccagcgcg aaattgccgg cctcctg
//
LOCUS       DATAOZ010000366         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2780582, whole genome shotgun sequence.
ACCESSION   DATAOZ010000366 DATAOZ010000000
VERSION     DATAOZ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2780582"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VHR64_09350"
     CDS             complement(<1..370)
                     /locus_tag="VHR64_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3303074.1"
                     /translation="MTTEMQFGVFVPQGWKLDLTEIEDPVEQFEAMTNVAKAADSEPG
                     IDSIWVYDHFHTVPQPELETTFECWTITATLARDTSRVKVGQMVGCNGYRNPALYAKI
                     ASTVDVASHGRLYAGIGAGWY"
     gene            553..897
                     /locus_tag="VHR64_09355"
     CDS             553..897
                     /locus_tag="VHR64_09355"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX3303075.1"
                     /translation="MIAVIFEVWPESGKADDYLAIAADLRPLLDTIDGFISIERFTSI
                     SDPTKYLSLSFWRDEEAVAEWRSQWEHREAQRRGRGDVFADYRLRVAAVARDYGMSER
                     EQAPADSRAIHG"
     gene            complement(928..1413)
                     /locus_tag="VHR64_09360"
     CDS             complement(928..1413)
                     /locus_tag="VHR64_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX3303076.1"
                     /translation="MQTEHMSSVRKGWREYDELAPGVTEAIGAIGDVIKGSALDKELL
                     ELVKIRASQLNGCAYCLQLHINKARDLNMAPERIDLIPVWPEVDVYSEREKAALRWTE
                     LVNDVAHGGVSDESYEGIHEYFSEEEVAILTAAIIEITAYNKLGAIYRMKPPIRSRAV
                     E"
     gene            complement(1449..1784)
                     /locus_tag="VHR64_09365"
     CDS             complement(1449..1784)
                     /locus_tag="VHR64_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX3303077.1"
                     /translation="MAEQITKLNLPDNHEHHSHASYAEHKAKIQTRLRRLEGQVRGVQ
                     RMIDEDQYCVDVLTQLSAIIAAARGVGMLVLEDHIRGCVIDAPETEREDRLDELTEAI
                     ERFTRTVAN"
     gene            complement(1838..>2502)
                     /locus_tag="VHR64_09370"
     CDS             complement(1838..>2502)
                     /locus_tag="VHR64_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX3303078.1"
                     /translation="PVAMGALYPFTGTLLSPILAAAAMAMSSVSVVTNALRLRGFKRP
                     ASAQEILHPPLRRRIVEVGYLAGIGLVAIAIGALAIVYAPDESSAEMGMGGSADAAAL
                     AVPDRTIQIETTDQLRFSPDQITVRKGETVAFVITNSGDLPHEFVIGDEAVQAEHEAE
                     MATGEEDMNAMGDKPYAVDVPAGETVTLVYTFDEEGTTLIGCHVPGHYGAGMKGTIVV
                     SD"
BASE COUNT          425 a          836 c          756 g          485 t
ORIGIN      
        1 cataccagcc ggcgccgatg cccgcataaa ggcgaccatg actggcgaca tcgaccgtgg
       61 aggcgatttt ggcgtagagg gccggattgc gatagccgtt gcagccaacc atctgcccaa
      121 ccttgacccg gctggtatca cgcgccaggg tggcagtgat cgtccagcac tcgaaggtcg
      181 tctccagctc gggttgggga accgtatgga aatggtcgta gacccagatg gagtcgatcc
      241 cgggctcgga atcggctgcc ttcgcgacgt tggtcatcgc ctcgaactgc tcgaccgggt
      301 cctcgatctc ggtcaggtcg agcttccacc cctgcggcac aaagacccca aactgcatct
      361 ccgttgtcac gtcacacatc tcctggcatg gcaaacagcc ccataaccac gagcgtaccg
      421 cgatctcggg catcaaacgg gcaggactgc tacgggaaag cgcatcgcta tgcgacgcta
      481 ccaaggcatc gatacgcggg ttcaattgat cccacatgtc gcgaccggct ttggatcggg
      541 gaggagagag gcatgatcgc cgtcatattc gaggtttggc cagagagcgg gaaggcggac
      601 gactacctgg ctattgccgc cgatttgcgg ccgctgcttg acactatcga tggcttcatc
      661 tcgatcgagc gcttcaccag catttccgac cccaccaaat acctctcgct ctctttctgg
      721 cgggatgagg aggcggttgc cgagtggcgt tcacaatggg agcaccgcga ggctcagcgt
      781 cggggacgag gcgacgtttt tgccgactac cggctgcggg tcgcggccgt cgcccgagac
      841 tatggcatgt ccgagcgcga gcaagccccg gccgacagtc gcgcaatcca cggctgaccg
      901 aagcaagcct ccgcgtcttc tcctcgacta ctcgactgct cgactccgga tcggcggctt
      961 catccggtag atcgcgccga gcttgttgta ggcggtgatc tcgatgatcg ccgcggtcag
     1021 gatcgcgacc tcctcctcgc tgaagtactc gtggataccc tcgtaggact cgtccgagac
     1081 accgccgtgg gcgacgtcgt tgaccagctc ggtccagcgc agggccgcct tctcacgctc
     1141 gctgtagacg tcgacctccg gccagaccgg gatcaggtcg atccgctccg gcgccatatt
     1201 gagatcccgc gccttgttga tgtgcaactg gaggcaatag gcgcaaccgt tgagctgcga
     1261 ggcccggatc ttgaccagct cgagcaactc cttgtcgaga gccgagccct tgatgacatc
     1321 gccgatcgcg ccgatcgcct cggtcacgcc gggcgcgagc tcgtcatact cccgccaccc
     1381 cttccgcacg ctgctcatat gttccgtctg cattccttcg cgttcctgtc tatcgcgtca
     1441 atacgatctc aattagccac ggtccgggta aagcgctcaa ttgcctccgt cagctcatcg
     1501 aggcgatcct cccgctccgt ctccggggcg tcgatcacgc agccgcggat gtgatcctcg
     1561 agcaccagca tcccgacgcc tcgcgccgcg gcgatgatcg ccgagagctg ggtcaggaca
     1621 tcgacgcagt actggtcctc gtcgatcatc cgctgcacgc cgcgcacctg cccctccagc
     1681 cgccgcaacc gggtctggat cttcgccttg tgctcggcat acgaggcatg gctgtgatgc
     1741 tcgtgattgt ccggcaggtt cagtttggtg atttgctccg ccatgttcat tctccgttgc
     1801 tgagtgatta gtggatagtg attagtgaat agagccacta atcactaacc actatcgtcc
     1861 ccttcatgcc cgcgccgtaa tggcccggga cgtgacagcc gatcagcgtc gtgccctcct
     1921 catcgaaggt atagacgagc gtgacggtct cgccggccgg gacgtcgacg gcgtagggct
     1981 tgtcgcccat cgcgttcata tcctcctcgc cggtcgccat ctcggcctcg tgctcggcct
     2041 ggaccgcctc gtcaccgatc acgaactcgt gcggcaggtc gccgctgttg gtgatcacga
     2101 acgcgacggt ctcgcccttc ctgacggtga tctggtccgg gctgaagcgg agctgatcgg
     2161 tcgtctcgat ctggatcgtg cggtccggca cggccagcgc ggctgcatcg gcgctcccgc
     2221 ccatgcccat ctcggcgctg ctctcgtccg gcgcgtagac gatcgccagc gcgccgatcg
     2281 cgatcgcaac cagaccgatc ccggccaggt agccgacctc gacgatccgg cgtcggagcg
     2341 gcgggtgcag gatctcctgc gcgctggccg gtcgcttgaa gccgcgcagc cggagcgcgt
     2401 tcgtcaccac gctgacagag ctcatcgcca tcgcggccgc ggccaggatc gggctcagca
     2461 acgtgccggt gaatgggtag agcgcgccca tcgcgaccgg ga
//
LOCUS       DATAOZ010000367         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6726768, whole genome shotgun sequence.
ACCESSION   DATAOZ010000367 DATAOZ010000000
VERSION     DATAOZ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6726768"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VHR64_09375"
     CDS             complement(<1..395)
                     /locus_tag="VHR64_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3303079.1"
                     /translation="MRKHVVLVVDDEPAIVRLVRAKLQSDGRAVISANSGEEALKVLE
                     DERPDLIVLDVMMPGIDGFETLRRIRTGAFSNQVPVIMLTARASDADRLKGLNAGADD
                     YMSKPFNPDELAARVTAVLRRTAGAVTASG"
     gene            complement(392..2338)
                     /locus_tag="VHR64_09380"
     CDS             complement(392..2338)
                     /locus_tag="VHR64_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3303080.1"
                     /translation="MSSDTKAELQESIQPALLSSIIGSAMDAIIALDSEQRIVLFNPG
                     AEQVFRCPALHAIGDKLDRFLPERYRNTHRKFVEQFGITQTTSRSMGHLRPLAAMRAD
                     GEEFPIEATISNVEIDGRTYYAAIVRDISGRREAEIALQKQAALLDLAYDAIFTWDWG
                     GAITSWNRGAERLYGYSRDEAIGQISHLLLKTTHPEGVSAVHSSLYEHGVWEGELTHT
                     RKDGSEVVVESRHVIAHDGDDSYVLEANRDATESKRAEEERARVMAQEAAARAEAAAA
                     AAERDRLHEILSRLPSGVYIVQSLDRSLTFANSPFRDLIHMPGTSGDQSPGYGRDFSF
                     LRADGTPLPVNERPGLRAMQGERVYNQQLLLERKDGSLVPITAHAAPLGADIFGSTHA
                     IVVIQDVAQLRQAEQLKDDFLALVSHEFRTPLTAIHGGAHLLSNAGDSLDEETKRELL
                     ADVVAESERLDRMLGNMLSLANVMAGRLPVVTEPVLLAPLARRAAEAISNRSPLHAFF
                     VDIPANFPPVEADPDLLDQVIRNLYENAVKYAPAGGPIRTTASHDGESVTITITDTGI
                     GIAPEHVDTVFERFRRVGGDPRVRGMGLGLYLSRHLVEAQGGHIWVTSPGPGLGASFS
                     VTLGIARDWSDDDAPGDESAEGEG"
BASE COUNT          460 a          788 c          793 g          476 t
ORIGIN      
        1 ccggacgccg tcacggcgcc ggccgtgcga cgaagaaccg cggtgacgcg ggcagcgagc
       61 tcgtccggat tgaatggctt ggacatgtag tcgtcggcgc cggcattaag ccccttgagg
      121 cgatccgcgt cgctggctct cgcggtgagc atgatcactg gcacctggtt cgaaaaggcg
      181 ccggtgcgaa ttcgccggag cgtctcgaac ccgtcgattc cgggcatcat cacgtcgagc
      241 acgatgaggt cggggcgctc gtcttccaga accttgaggg cttcttcgcc actgttcgcg
      301 gaaatgaccg cgcggccatc cgattgcagc ttcgcccgca caagtcggac aatcgccggc
      361 tcgtcatcga caaccagaac gacgtgcttc ctcatccctc accctcggcg gactcatcac
      421 ccggtgcatc gtcatcggac caatcccgcg caatacccag cgtaacggaa aaactcgcgc
      481 caagtcccgg accgggcgat gtcacccaaa tatggccgcc ttgcgcctcg accagatgcc
      541 ggctcagata caggccaagg cccatcccgc gcacgcgcgg gtcgccaccg acgcggcgga
      601 accgctcgaa gaccgtgtcg acatgttccg gcgcgatgcc gatgcctgtg tccgtgatcg
      661 tgatcgtgac cgactcgccg tcgtgactcg ccgtcgtgcg gatcggtccg ccggccggcg
      721 cgtacttgac cgcattctcg tacaggttcc ggatgacctg atcgagcagg tccggatcgg
      781 cttcgacagg cggaaagttg gccgggatat cgacgaagaa tgcgtgaagc gggctgcggt
      841 tgctgatcgc ttcggccgcc cgcctcgcca agggtgcaag caggactggc tcggtgacga
      901 ccggcagtcg acccgccatg acgttggcaa gcgagagcat gttgccgagc atccgatcca
      961 gccgctcgct ctctgcaacg acatcagcca gcaactcgcg cttggtctct tcatccagcg
     1021 aatcgccggc gttggaaagc aggtgcgctc cgccgtggat tgccgtcaga ggcgtccgga
     1081 attcgtggga aacgagtgcc agaaagtcgt ccttgagctg ttcggcttga cgtaactgtg
     1141 cgacatcctg aatcacgacg atcgcgtggg tgcttccaaa aatgtccgca cctagcggtg
     1201 ccgcatgggc ggtaatcggg accagcgatc catcctttcg ctccaggagg agttgctggt
     1261 tatagacacg ttcgccctgc atggcgcgaa gtcccggccg ttcgttgacc ggaagcggcg
     1321 tgccgtccgc gcgcaggaac gaaaagtccc gtccataccc tgggctctga tcccccgagg
     1381 ttcctggcat gtgaatcaag tcgcggaagg gcgaattggc gaaggtgagg gaccggtcta
     1441 atgactgaac gatgtagaca ccactcggta gccgactcag gatctcgtgc aaacggtcgc
     1501 gctccgcagc cgcggctgcc gcttcggcgc gggctgccgc ctcctgggcc atgaccctcg
     1561 cccgttcctc ctcggcgcgc ttgctctcgg tggcgtcgcg gttggcttcg aggacgtacg
     1621 agtcatcacc gtcatgcgcg atcacgtgtc gactctcgac gacgacctca gagccatcct
     1681 tgcgcgtgtg ggtcagctca ccttcccaga caccatgctc ataaagtgac gagtgaaccg
     1741 ctgacactcc ctcagggtgg gtcgtcttga gcagcagatg gctgatttgg ccgattgcct
     1801 catcacgcga gtaaccataa agacgctccg cgccgcggtt ccacgaggtg atcgctccgc
     1861 cccaatccca ggtaaagatg gcgtcatagg cgaggtcgag gagggcggct tgcttctgaa
     1921 gagcgatttc cgcttctcgt cggccactga tgtcccggac gatcgcggcg tagtaggtcc
     1981 ggccgtcgat ttcgacattc gagatcgtgg cctcgatcgg gaactcctcg ccgtcagcgc
     2041 gcatcgcggc gagtgggcgc agatgaccca tcgagcgcga ggtggtctgg gtaatgccga
     2101 attgctcgac gaatttccgg tgcgtattgc gatagcgttc aggcagaaaa cggtcgagct
     2161 tgtcgccgat ggcatgtaat gccgggcagc ggaatacctg ctcggcaccc gggttgaaga
     2221 gcacgattcg ctgctcggaa tcaagcgcga tgatcgcgtc catcgccgac ccgatgatcg
     2281 agctgaggag ggcaggctga atcgattcct gcagttcggc tttggtgtca gagctcatgc
     2341 acccgtccag ggctcagcgc tgcgagtccg cgcatgcgat ggtcaagtgt cgcaatggcg
     2401 atccggcatg ccggatcgcc attggaatct tagcgcagca ggcatcggat cctggttctg
     2461 gtccgggcaa gggtgtccta cctggtgacg aaaccgccat cgacggcaat cgcctgg
//
LOCUS       DATAOZ010000368         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5674515, whole genome shotgun sequence.
ACCESSION   DATAOZ010000368 DATAOZ010000000
VERSION     DATAOZ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5674515"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VHR64_09385"
     CDS             complement(<1..618)
                     /locus_tag="VHR64_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3303081.1"
                     /translation="MAKKRVLITGVAGVVGQACVAHLSDRYELSGLDIKDPGNVPTLV
                     ADIADLNAILPAFEGMDAVIHLAGNPSVHSPWNDVFNINIQGTYNAYEACRIQGVTCL
                     VFASSNHTIGQYEEEGMPEIYDLDDPRVYDETVEVRPDSLYGVSKLFGEAVGRYYQEY
                     FGVRSYNLRIGSVRADDNPRDPSVPEGSFWLDLTPEQKYDRLRATW"
     gene            716..1609
                     /locus_tag="VHR64_09390"
     CDS             716..1609
                     /locus_tag="VHR64_09390"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX3303082.1"
                     /translation="MNNLPFDRPGQFWKTNLHSHTTRSDGKLTPTELTSIYREQGYHA
                     LAITDHFMERFGFPIVDTTGFRTDNFTTLLGAELHGPGLMLGDWHILAVGLPPDFAPS
                     TNGETGPEIANRASDAGAFVAIAHPAWYSLFYEKAKEIDFADAIEVFNTTCLYDNDRG
                     ESWYLADQFLMTGRRPFAIATDDAHCSIRPDTFGGWTMIKSESLDPESLLAALKAGHF
                     YASTGPDIHNIAIEGDKLIVESDPARSIYATGAGALHRDVTGNGVTRAEFDIEPFTHS
                     YARVTILTETGKAWSNPIFFD"
     gene            complement(1750..2772)
                     /locus_tag="VHR64_09395"
     CDS             complement(1750..2772)
                     /locus_tag="VHR64_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303083.1"
                     /translation="MSDLVDETWDFGLEEGESFEPLDEDEEFDLSQPHVMTVLGPIDP
                     GALGFTLHHEHVFNFINPLAANDPDQILDDPAASLTDLEVYFGAGGRAIVDMGPADYG
                     RSITDLLWIAQRAPVHIVQVTGHHKHLIAAPLVGESSVADIVAHSVAELTTGIEGTAA
                     RAGLIKAGTSLDEISEVERRVLIAAAEAQKATGAPISTHTDYGTMALAQIRVLTEAGA
                     DPRRVILCHLDFQLGNLPYLREVLATGAFVSFDHWSKVRHSSDGARAAVFFRLADEGY
                     LDQLLISGDLARKSNHTGYGGTPGFEYFLDHVPLMLMDAGFDAPSVRKIFVDNPARAL
                     TIIQPT"
     gene            complement(2769..3248)
                     /gene="moaC"
                     /locus_tag="VHR64_09400"
     CDS             complement(2769..3248)
                     /gene="moaC"
                     /locus_tag="VHR64_09400"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX3303084.1"
                     /translation="MSDERRLTHFDEAGRARMVDVGDKAVTARRAVATGRVDMQPETV
                     ELIRTNGVKKGDPLAVAQVAGIMAAKRTHELVPMCHPLLLTKVALELELDDAGVSIRA
                     TVETRGQTGVEMEALTAVSVAALTVYDMVKAADRGMMITGIGLAEKSGGKSGEWVRA"
     gene            3333..>3651
                     /locus_tag="VHR64_09405"
     CDS             3333..>3651
                     /locus_tag="VHR64_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303085.1"
                     /translation="MSSFFRAARRIAENPATRDMVKQQVDRVTRTTESSGAAERGAGA
                     ILGALANGKASLSALNELGRQLQSRGSWLNGLQQLVSQQSSDQLKSVANQAVSALPES
                     VRTD"
BASE COUNT          639 a         1308 c         1068 g          636 t
ORIGIN      
        1 ccaggttgcc cgcagccggt cgtacttctg ttccggcgtc agatcgagcc agaatgagcc
       61 ttcgggcacg ctcgggtcgc gcggattgtc gtccgcccgg accgagccga tccgcaggtt
      121 gtacgagcgg accccgaagt actcctggta gtagcgcccc actgcctcgc cgaagagctt
      181 gctgacgccg tacagcgagt ccggtcgaac ctcgacggtt tcatcgtaaa ctcgcggatc
      241 gtcgaggtcg tagatctccg gcatcccctc ttcctcatac tgcccgatcg tgtggttgga
      301 cgacgcgaag accaggcacg tgacgccctg gatccggcag gcctcgtacg cgttgtaggt
      361 tccctggatg ttgatattga agacgtcgtt ccacggcgag tggaccgatg ggttcccggc
      421 caggtgaatg accgcatcca tcccctcgaa cgccggcaag atcgcgttca ggtcggcgat
      481 gtccgcgacc agcgtcggca cgttgcccgg gtccttgatg tcgagcccgc tcagctcgta
      541 ccggtccgac aggtgcgcca cgcacgcctg ccccaccact ccggccacgc cggtaatcaa
      601 tacgcgcttc ttcgccaaga tcgactccct tgctccgccc aaaccagcca tcacgccggg
      661 tatcctacca accagcgaat tccggcatgt gacagcaaga acaggatcac gatcggtgaa
      721 caacctcccc tttgaccggc cagggcagtt ctggaagacc aacctccatt cgcacaccac
      781 ccgctcagac ggcaagctga cgccgaccga gctgacgagc atttaccggg agcagggcta
      841 ccacgccctc gcgatcaccg atcacttcat ggagcggttc ggcttcccga tcgtcgatac
      901 caccggattc cgcaccgaca acttcacgac cctgctcggc gctgagctgc atggccccgg
      961 cctgatgctt ggcgactggc acatcctcgc cgtcggtctc ccgcccgatt tcgccccgtc
     1021 gacgaacggc gagaccggtc cggagatcgc gaatcgggcc agcgatgccg gcgcgttcgt
     1081 cgcgatcgcc catcctgcct ggtattcgct tttctatgaa aaggcgaagg aaatcgactt
     1141 cgccgacgcg atcgaggtct tcaacacgac ctgcctctac gacaacgacc gcggcgaaag
     1201 ctggtacctg gcggaccagt tcctgatgac cggccgccgc cccttcgcaa ttgcgaccga
     1261 cgacgcccat tgctcgatcc ggcctgacac gttcggtggc tggaccatga tcaagagcga
     1321 gtcgctcgat cccgagtcct tgctggccgc gctcaaggct ggccacttct acgcctcgac
     1381 cggtcccgat atccacaaca tcgcgatcga gggcgacaag ctgatcgtcg aatcagaccc
     1441 ggcccgctcg atctacgcca ccggcgccgg cgccctccac cgcgatgtca ctggcaacgg
     1501 cgtgacccgc gccgagttcg acatcgagcc cttcacccac agctacgcgc gagtcaccat
     1561 cctcaccgaa accggcaaag cctggtccaa ccccatcttc ttcgactagg cgcacgcaaa
     1621 ccgtagtggc ggggctccgt acccgcctgg ggctgcctgt acccacggct cgagatgtcg
     1681 gaccgatcca cggtcagcct cggagaggct tcgtgttgtc agcccggggt ttcaactccg
     1741 gggtagtcgt catgtcggct ggatgatcgt cagcgcccgc gccgggttgt cgacgaagat
     1801 cttgcgaacc gacggggcgt cgaagccggc gtccatcagc atcagcggca cgtggtcgag
     1861 gaagtactcg aaaccgggcg tcccgccgta cccagtgtgg ttcgacttcc gggccagatc
     1921 gcccgagatc agcagctgat cgagataccc ctcgtccgcg agccggaaga agaccgccgc
     1981 ccgcgcccca tccgacgaat gccgcacttt cgaccagtga tcgaacgaca cgaacgcccc
     2041 ggtcgccagc acctcgcgca gatacggcaa attcccgagc tgaaagtcga gatgacagag
     2101 gatgacccgg cgcggatcag ccccggcctc ggtcagcacc cgaatctggg ccagcgccat
     2161 cgtgccgtag tcggtatggg tcgagatcgg cgccccggtc gccttctgcg cctcggccgc
     2221 cgcgatcaga acccgtcgct cgacctcgga gatctcgtcc aggctcgtcc cggccttgat
     2281 cagccccgcc cgtgccgccg tcccttcgat ccccgtcgtc aattccgcca cgctgtgcgc
     2341 gacgatgtcc gcgaccgagc tctcgccgac cagcggggcc gcgatcaggt gcttgtggtg
     2401 cccggtcacc tgcacgatgt ggaccggcgc ccgctgcgcg atccagagaa gatcggtgat
     2461 cgaccgccca taatcggccg gccccatgtc gacgatcgcc cggcccccgg ccccgaagta
     2521 gacctcgaga tcggtcagcg aggcggccgg atcgtcgagg atctgatccg ggtcgttcgc
     2581 ggctaacggg ttgatgaagt tgaagacgtg ctcgtggtgg agcgtgaagc cgagcgcgcc
     2641 cggatcgatc ggcccgagca cggtcatcac gtgcggctgg ctcagatcga actcctcgtc
     2701 ctcgtcgagc ggctcgaagc tctcgccctc ctcgaggccg aaatcccatg tctcgtcgac
     2761 caagtcgctc acgcccgtac ccactcgccc gacttgccgc ccgacttctc ggccagtccg
     2821 atccccgtga tcatcatccc gcggtcggcc gccttgacca tgtcgtagac ggtgagggct
     2881 gccacactca cggccgtgag cgcctccatc tcgacgccgg tctggccgcg cgtctcgacg
     2941 gtcgcccgga tcgacacacc agcatcgtcc agctcgagct ccagggcaac tttggtcagc
     3001 agcagcgggt ggcacatcgg caccagctca tgcgtccgct tggcagccat gatcccggcc
     3061 acttgcgcca ccgccagcgg gtcgcccttc ttgactccgt tggtccggat cagctcgacc
     3121 gtctcgggct gcatgtcgac ccgcccggtc gcgaccgcgc gccgggccgt aaccgccttg
     3181 tcgccgacat cgaccatccg cgcccggccg gcctcgtcga aatgagtcaa tctgcgttcg
     3241 tcgctcattg ccgtgccctc ctcaggcggt atgctggagt gcatgatacg ggcatgatcg
     3301 gttctgttcg tgcctcaccg aaaggtgtag ttgtgagctc attctttcgc gccgctcgtc
     3361 ggatcgccga aaatccggcc acgcgcgaca tggtcaagca acaagtcgac cgcgtgaccc
     3421 gcaccaccga aagttccggg gcagcggagc gcggcgccgg cgcgattctc ggtgcattgg
     3481 ccaatggcaa ggcctcgttg tccgccctca acgagctcgg acgacaactc cagtcccgcg
     3541 gatcatggct gaacggcctg caacaactcg tcagccaaca atcgtcggac cagctcaaat
     3601 ccgtcgcgaa ccaggcagtc tcggctctgc cggaatcggt gcgcaccgat c
//
LOCUS       DATAOZ010000369         3910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3568290, whole genome shotgun sequence.
ACCESSION   DATAOZ010000369 DATAOZ010000000
VERSION     DATAOZ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3568290"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VHR64_09410"
     CDS             complement(<1..279)
                     /locus_tag="VHR64_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303086.1"
                     /translation="MLGIVLAVGIMTASNELITHDRLMDDIGMNWPSLTHLRLWTIPA
                     ATFVQASPGVAWTMLLLVGLPMIALEFLAGSWRALVTFLLCDWLTAPVT"
     gene            complement(318..2927)
                     /locus_tag="VHR64_09415"
     CDS             complement(318..2927)
                     /locus_tag="VHR64_09415"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX3303087.1"
                     /translation="MEQFLRRVWRLIGAAPTLLWGSALVLLSGIFIWRARSELSNIGA
                     ELRTAKLQWLIALFLLAILTQALNGLKLQLLMRRLGCRVPYPEVTSAQLQRQMILTVV
                     PVGSAPSAVLFARRFARFGATTPIMLTALMLFSLLGHASFVAVMIPVFVWLAIGGSLS
                     TTVLIAGVALVAAVLVISGVGLITLQRGSIPGWAHRRLPHRVTDFIGEALATGVPPRG
                     LAAPFAIAISVDLVGVAMLWTALRALDAPLSLEIAAGGYVIGTLFLMIAPLFQGIGIV
                     EITMALALERLGVPTAQAFGATLLYRVGDVWVPLAVGIAIQIRAQEQLRRLPRNLPAL
                     MTGATGLLSVLSVMAPTIPARFNRLEDYAFFDPHDVSRTFSLVAGALLIALSLALLRR
                     RQVAWWCAVGLLSFLIVSHLFKRHDQIVAVIAGVNLAVLLITRRRFRVRSDVPTMRRG
                     VALFFGSLAFALAYGTLGFWLLDRREFNINFSIGRSLEETLSLFFNLGGTDLDPHTRY
                     ADWFIDSVSVVGVISLFAALVSLLRPVVWRRRTLPHERVEADALINRYGRSSLDEFKV
                     WPDKIYFFGSDRRSVICYGVRGAVAVALGDPVAPDDAAFERVVDEFLDFCDANAWRVA
                     FHQVGPEHLATYREADLDALKVGEDAIVDLATWTLSGHAMKPFRAVVNRLAREGFTVC
                     RHDPPLPESLLAELRAVSDAWLTLPGRRERGFTLGQWDDAYVRRSPVFTLSSSEGRIV
                     AFINLIRDGVPGEGTFDLMRHRPDAPNGSMDVLLVRLAEYLRDAGYSRMSLGMVPFAD
                     VGTGEADPVLERGLRLLVERMERFFSYRGLRMYKEKFHPIWEPRYLIFPTGVALPQIA
                     LAIIQLTEGGSSG"
     gene            complement(3053..3310)
                     /locus_tag="VHR64_09420"
     CDS             complement(3053..3310)
                     /locus_tag="VHR64_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303088.1"
                     /translation="MAPIEGRILVGAQQVVDTWFQADNRGPFTRAVTRAIERQWPGIG
                     ADDPRRLAFMRVAEAIRVGRGHAFADPAPLVRIAQKTLYRT"
     gene            complement(3352..3804)
                     /locus_tag="VHR64_09425"
     CDS             complement(3352..3804)
                     /locus_tag="VHR64_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303089.1"
                     /translation="MIRRAALTWAAMIPVAIFNGTFRELALKPVLGDQTARQASVATA
                     SVAFLVLVYLIMREHVVDETDRRLLEVGIAWLAATIVFEFGFGHFVDGKSWGDLLHDY
                     NIFAGRLWPVFLGIEMLAPLIVKRFMLWQGTDIGRGQRPHGPQAQGVH"
BASE COUNT          787 a         1418 c         1182 g          523 t
ORIGIN      
        1 ggttaccggc gcggtcagcc aatcgcagag caggaaggtg accagcgcgc gccagctccc
       61 ggccaggaac tccagcgcga tcatcggcaa tccgaccagc agcagcattg tccaggccac
      121 tccgggcgat gcctgtacga acgtcgcggc cgggatggtc cagagccgaa ggtgcgtgag
      181 agacggccag ttcatcccga tgtcgtccat caaccggtcg tgcgtgatca gctcgttgct
      241 cgcggtcatg atcccgacgg ccagaacgat tccgagcacg agcaacgtcg ctggaaacca
      301 gcggctgcac cggcgcctca gccagacgac ccgccctcgg tcagctggat gatcgccagc
      361 gcgatctgcg gcaaggccac gccggtcgga aagatcaggt atcgcggctc ccatatcggg
      421 tgaaacttct ccttgtacat ccgcaacccg cgatacgaga agaaccgctc catccgttcc
      481 accagcaatc gcaagccgcg ctccaggacg ggatccgcct cgccggtccc gacatcggcg
      541 aacgggacca tcccgagcga catccggctg tatcccgcgt cgcgcagata ctcggccaac
      601 cgcaccaaca gcacatccat gctgccgttc ggcgcatcag ggcggtgccg catcaggtcg
      661 aaggtcccct cacccggcac gccgtcccgg atcaggttaa taaacgccac gattcggccc
      721 tcggatgaac tcagcgtgaa tacggggcta cgccgcacgt aggcatcgtc ccactgcccg
      781 agggtgaatc cgcgctcccg ccgccccggc aacgtcagcc acgcgtccga gaccgcccgc
      841 agctcggcca gcagggattc cggcagcggc ggatcgtggc ggcaaaccgt gaacccctcc
      901 cgcgccaacc ggttgaccac ggcccggaac ggcttcatcg catggccgct caaggtccag
      961 gttgccaggt cgacgatcgc gtcctcaccg accttgagcg cgtcgagatc cgcttcccgg
     1021 taggtcgcca ggtgctccgg cccgacctgg tggaaggcca cccgccacgc gttcgcgtcg
     1081 cagaagtcga ggaactcatc caccacccgc tcgaaggccg catcgtccgg cgccaccgga
     1141 tcccccaacg ccactgccac cgcgccgcga acgccgtagc agatcaccga tcgccggtcc
     1201 gacccgaaga agtagatctt gtccggccag accttgaact cgtcgagcga cgagcgcccg
     1261 taccggttga tcagcgcgtc agcctcgact cgctcgtgcg gcagggtccg gcggcgccaa
     1321 accaccggcc gcagcagcga caccaacgcc gcaaacaggc tgatcacccc gacgaccgag
     1381 accgaatcga tgaaccagtc ggcatagcga gtgtgcggat cgagatcggt gccaccgaga
     1441 ttgaagaaga gcgagagcgt ctcctcgagg ctgcgaccga tcgagaagtt gatgttgaac
     1501 tcgcgccggt ccagcaacca gaagccgagc gtgccgtacg ccaacgcgaa ggccaggctg
     1561 ccgaagaaga gcgccacgcc gcgccgcatc gtcggcacat cgcttcgcac ccggaaccgc
     1621 cgccgcgtga tcaggagcac ggccaggttg acccccgcga tcaccgccac gatctggtca
     1681 tgccgcttga acaggtggct gacgatcagg aagctcagca gtccgaccgc gcaccaccac
     1741 gccacctgcc gccgccgtaa cagcgccagc gagagcgcga tcaggagcgc gccggccacc
     1801 agcgagaatg tccgcgagac atcgtgcgga tcgaagaagg cgtaatcctc gagccggttg
     1861 aagcgggccg ggatcgtcgg cgccatcacc gagagcacgc tcagcaaccc ggtcgcacca
     1921 gtcatcagag ccggcaagtt gcgcggcaac cggcgcaatt gctcctgcgc ccggatctgg
     1981 atcgcgatcc cgaccgccag cggtacccag acatcgccga cccggtagag cagagtcgca
     2041 ccgaacgcct gggctgttgg cacgcccagc cgttcgagcg cgagggccat cgtgatctcg
     2101 acgatcccga tcccctggaa gagcggcgcg atcatcagga agagcgtgcc gatcacgtac
     2161 ccaccggccg cgatctccag cgacaacggc gcatcgagcg cgcgcagggc cgtccaaagc
     2221 atcgcgaccc cgaccaggtc gacgctgatc gcgatcgcga acggcgccgc caatccccgt
     2281 ggcggcacac cggtcgcgag ggcctcgccg atgaagtccg tcacccggtg cggcaagcgc
     2341 cgatgggccc agcccggaat cgatccccgc tgcagcgtta tcagacccac gccggaaatc
     2401 accagcaccg ccgcgaccaa cgccaccccc gcgatgagca cggtggtcga cagcgagcca
     2461 ccaatcgcca accagacgaa cacggggatc atcacggcga cgaacgacgc atggccgagc
     2521 aagctgaaca gcatcagggc cgtcagcatg atcggggtcg tcgccccgaa ccgggcaaac
     2581 cggcgcgcga agagaaccgc ggacggagcg gacccgactg gcacgacggt caggatcatt
     2641 tgtcgctgta gctgcgcgga ggtaacctca gggtacggca ctctgcagcc gaggcggcgc
     2701 atgagcagct gcagcttcag gccgttcagc gcctgcgtca ggatggcgag gaggaaaagc
     2761 gcgatcagcc actggagctt ggcggtccgc agctccgcgc cgatattgga aagctcagat
     2821 cgcgcgcgcc agatgaagat gccgctcagc aggaccagcg ccgaacccca gagcagcgtc
     2881 ggcgccgcgc cgatcagccg ccaaactctc cgcaggaact gctccatgat tcaggccggt
     2941 tctcgctcat gaccgcttgt ggcccgtacg atttgaacca taacaccggg aaatggcgcg
     3001 ggccggaacg ttcgcacgtc ccggcccgcc cagccacacg atctggaaac agctatgttc
     3061 gatagagcgt cttctgggcg attcggacca gcggcgccgg gtccgcaaag gcatgcccgc
     3121 gacccacgcg gatcgcctca gccacccgca tgaaggcgag gcggcgcgga tcgtccgcgc
     3181 cgatgcccgg ccattgccgc tcgatcgccc gggtgaccgc ccgcgtgaac gggccgcgat
     3241 tgtccgcctg aaaccaggtg tcgaccacct gctgcgcccc gaccaggatc cgcccctcga
     3301 ttggcgccat cgctctcctg accgcactct gattcggcat cggcgtcaac atcaatgcac
     3361 cccctgcgcc tgtgggccat gcggtcgctg cccgcgccca atatccgtcc cctgccacaa
     3421 catgaaccgc ttcacgatca gcggcgcgag catctcgatg cccaggaaga ccggccacag
     3481 ccggccggcg aagatgttgt agtcatggag caagtcgccc cagctcttgc cgtcgacgaa
     3541 gtgcccgaaa ccgaactcga agacgatcgt cgccgccagc caggcaatgc ccacctccag
     3601 caaccggcga tcggtctcgt cgaccacgtg ctcgcgcatg atcagataga ccagcaccag
     3661 gaacgcgacc gatgcggtgg ccacactcgc ctgccgcgcc gtctggtcgc ccagtaccgg
     3721 cttcagcgcc agctcccgga atgtgccgtt gaaaatcgcg accgggatca tcgcggccca
     3781 ggtcaacgcc gccctgcgaa tcatcgcctc gctcctcttg aggggtgcac aaccacgcat
     3841 ccacctccac aaccgagcat aggtgcgccg ccgtccaact cgcgtatcgc cggcgatcaa
     3901 ggcatcacaa
//
LOCUS       DATAOZ010000370         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8041960, whole genome shotgun sequence.
ACCESSION   DATAOZ010000370 DATAOZ010000000
VERSION     DATAOZ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8041960"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VHR64_09430"
     CDS             complement(<1..228)
                     /locus_tag="VHR64_09430"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3303090.1"
                     /translation="MAFSNDSCPSRMIKASPERVYRAFVDPAELVAWLPPGEMTGQIH
                     AFEARVGGGYVMSLYYPESETGNRGKTSARED"
     gene            complement(249..668)
                     /locus_tag="VHR64_09435"
     CDS             complement(249..668)
                     /locus_tag="VHR64_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX3303091.1"
                     /translation="MRVVVDSNFVVRLLTQLRPNPYAVLWQGWLENDIEVHSTLLLWW
                     EATNAFHRMALAGDISHTAPALLMQLVSELDIRYSDVRDDHISALEVAQRFKAPAAYD
                     AHYVVLAQRLDCDLWTADKKLHRLVSGQLPWVRLADD"
     gene            689..922
                     /locus_tag="VHR64_09440"
     CDS             689..922
                     /locus_tag="VHR64_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303092.1"
                     /translation="MVDEFVNVRIWAPIEILLKFARNLFLPSPPIFGKRCLPLAPKVV
                     NRDDRRHFPTATLDYIWLPSMFDCVKHFAQMNA"
     gene            complement(1077..>2006)
                     /locus_tag="VHR64_09445"
     CDS             complement(1077..>2006)
                     /locus_tag="VHR64_09445"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX3303093.1"
                     /translation="HGARGITCAKLGEAEVMVEGGVEDVLMAYPVVTELKINRLLALM
                     ERARMIVALDSLDAAEALSAAMSKHDRSLDVYVEVNTGQNRAGQIAGDDAVKLALDIA
                     RMPSLDVIGIMTHEGQTHTLPADKIAEGARAAGEKLVATAEAIRRAGLPISEVSVGST
                     PSSMYTPSVGGITEMRPGTYVFRDTTGFLYGLYGPDRCAARVLATVVSHPAKDRMIVD
                     AGSKTLALDKSHGHPGHGYIIGHPGSIIDRLSEEHGVVVIPEDDPGFTIGQRVEIIPN
                     HICPSVNLLDELNIVRDGQVIDVWPIAARGKVR"
BASE COUNT          370 a          656 c          577 g          403 t
ORIGIN      
        1 atcctcccgc gcggatgtct tgccacgatt cccggtctcg ctctcggggt agtaaagcga
       61 catgacatat ccgccgccga cccgggcctc gaacgcatga atctgcccgg tcatctcccc
      121 cggcggcagc cacgccacca actccgccgg atcaacaaac gcccggtaca cccgctccgg
      181 cgatgccttg atcatcctgg atggacacga gtcattgctg aatgccacgg cgcgaatcct
      241 gcgaaaagct aatcgtcggc gagtcgcacc catggcaatt gaccactaac cagtcggtga
      301 agtttcttgt cggcggtcca gaggtcgcaa tccaggcgtt gcgctagcac gacataatgc
      361 gcgtcgtaag ccgccggtgc cttgaacctt tgtgcgactt caagtgccga gatgtgatcg
      421 tctcgaacat ctgaatagcg gatgtccagt tccgacacta gctgcatcaa gagtgcgggt
      481 gccgtatgtg aaatgtctcc tgcgagcgcc atgcggtgaa atgcgttcgt ggcttcccac
      541 caaagcagca gtgtcgaatg cacttcaatg tcattctcca gccaaccttg ccagagaact
      601 gcgtacggat tcggacgtaa ctgcgtaagt aaacgcacca cgaaattcga atcgactacg
      661 actcgcattt cgatggttat cgctctccgt ggtcgatgag ttcgtcaacg tccggatatg
      721 ggcgcccatt gagattcttc tcaagttcgc gcgcaacctg ttcctgccgt cgccaccaat
      781 cttcggaaag cgctgccttc cgctggcgcc gaaagtcgtc aatagggatg accgccgcca
      841 tttcccgacc gccacgctcg attacatatg gctcccttcg atgttcgatt gcgtcaagca
      901 cttcgcccag atgaacgcgt aactgcgttg ccgttactcg ctttggttcc atgagctttc
      961 ttgtcggttc aatatccatc actcaatcca tcgatggatc gatcatatca aatagaggcg
     1021 gcaaagcttc gcccggcaac ctgggatctg atgctgattc agcggaccgt cagtcgctac
     1081 cggaccttgc cgcgggccgc gatcggccag acgtcgatca cttggccgtc gcggacgatg
     1141 ttcagttcgt ccagcaggtt gaccgatggg cagatgtggt tcgggatgat ctcgacccgc
     1201 tggccgatcg tgaagccggg gtcgtcctcc gggatgacca cgacgccgtg ctcctcgctc
     1261 agccggtcga tgatgctgcc cgggtggccg atgatgtagc cgtgtccggg atgaccatgg
     1321 ctcttgtcga gcgccagggt cttcgagccg gcgtcgacga tcatgcggtc cttcgccgga
     1381 tgactgacga ccgtggccag cactcgcgcc gcgcaccgat ccggaccata cagcccgtac
     1441 aggaagccgg tcgtgtcacg gaagacgtac gtgccgggcc gcatctcggt gatgccgccc
     1501 accgagggcg tgtacatcga ggagggagtc gagccgacac tgacctcgct gatcggcaaa
     1561 ccggcccggc gaatcgcctc ggcggtcgcg accagcttct cgccggccgc acgcgcgccc
     1621 tccgcgatct tgtcggccgg cagcgtgtgc gtctggccct cgtgggtcat gatcccgatc
     1681 acgtcgaggc tcggcatccg ggcgatgtcg agcgcgagct tgaccgcatc gtcgccagcg
     1741 atctgaccgg cccgattttg gcccgtgttg acctcgacat agacgtcgag cgagcggtcg
     1801 tgcttcgaca tcgccgccga aagcgcctca gccgcatcga ggctgtccag cgccacgatc
     1861 atccgggccc gctccatcag cgccagcaac cggttgatct tgagctcggt cacgaccgga
     1921 taggccatca gcacatcctc gaccccgccc tcgaccatga cctcggcctc gccgagcttg
     1981 gcgcaggtga tcccgcgcgc tccgtg
//
LOCUS       DATAOZ010000371         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_938043, whole genome shotgun sequence.
ACCESSION   DATAOZ010000371 DATAOZ010000000
VERSION     DATAOZ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938043"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VHR64_09450"
     CDS             <1..803
                     /locus_tag="VHR64_09450"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX3303094.1"
                     /translation="LLLRNRINLPALSDQLGHGFCGNADYLGFIRTGKNDGSLDASRG
                     PTITSYVEKRRNGASAAGGASKVLLVEDGGYPVLGDWLGEAFRLQAARRIAAVALTTL
                     AARLTNKSRTRVSAKLSQIVGDSYPSRSLLPMLGMGQDMPRGVMGLRNGDLEIAWRES
                     YSQSAFDGIRQTMNEVATGLKGKFQDGPSTMLSRMITVHPLGGVPMGRTPEQGVVDSY
                     GEVFGYPRLFVADGSIMPGPVGVNPALTIAAVAERASCRVIEKAGDAR"
     gene            800..>1593
                     /locus_tag="VHR64_09455"
     CDS             800..>1593
                     /locus_tag="VHR64_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX3303095.1"
                     /translation="MITNNGFKNPAPRRALIMAGGALKVAFQAGVLQVWLDEARTPDG
                     RRLDFELADGASGGVFNLAMWCQGLTGRQIADNWRRTQPFRGISFNWRQWVPAPASLF
                     TYEGFRKHVFSGTWNLDWPAIRNTERVASFNLFNVNRQQHQVRRASQMDEAALVSAVT
                     LPLWFPPVRLEDGAGEASYMDAMFATDANIEQAIADGADEVWVIWTVSQRGTVRPGFT
                     PGYFQMIEAIANSRIRAVLDRIERNNAAIEAGQPGEFQRRIEVKWL"
BASE COUNT          289 a          492 c          519 g          293 t
ORIGIN      
        1 acttgttgct tcgcaaccgc atcaacctgc cagcgctcag cgaccaactt ggccacgggt
       61 tctgtggcaa cgccgactat ctgggcttta tccggaccgg gaagaacgac ggctcgctgg
      121 atgcatctcg cggtccgacc atcaccagtt acgtcgagaa gcggcgcaac ggcgcttcgg
      181 cggccggtgg agcctccaag gtcctgctcg tcgaggacgg cggctatccg gttcttggtg
      241 actggctggg cgaggcgttc cggctgcaag cagcacggcg aatcgccgcc gtagccctga
      301 ccacgctggc cgcgcgtctg acgaacaagt cccggacccg agtcagcgca aagttgtccc
      361 agattgtcgg cgactcctac ccatcgcgca gcctgctacc gatgctcggc atgggtcagg
      421 acatgccgcg aggtgtcatg ggacttcgca acggcgactt ggagatcgcc tggcgggagt
      481 cctattcgca gtccgcattc gatgggatcc gacagacgat gaatgaggtc gcaaccggtc
      541 tcaagggaaa gttccaggac ggacccagca cgatgttgag ccggatgatc acggtgcatc
      601 cgctgggcgg agttccgatg ggcaggacgc ccgagcaagg cgtggtcgac agctacggcg
      661 aggtcttcgg atacccgcgg cttttcgtcg ccgacggttc gatcatgccg gggccggtcg
      721 gcgtcaatcc agcgctgacc attgcggctg tcgcagagcg cgcctcttgt cgcgtgatcg
      781 agaaagccgg agatgcccga tgatcactaa taacggattc aagaacccgg ccccgcgccg
      841 agcgctgatc atggccggag gtgctctcaa ggtcgcgttt caggccggcg tgctgcaggt
      901 ctggctggac gaggcgcgta cgcccgacgg tcgtcgtctc gacttcgagc tggccgatgg
      961 agctagcggt ggggtgttca acctggccat gtggtgtcaa ggcctcaccg gccggcagat
     1021 cgccgacaac tggcgacgga cccagccgtt ccgtggcatc tcattcaact ggcggcagtg
     1081 ggtgcccgct ccggcttcgc tctttactta cgaaggcttc cgcaaacacg ttttctccgg
     1141 cacctggaac cttgactggc ccgcgatcag gaacaccgaa cgggtcgcct ccttcaactt
     1201 gttcaacgtc aaccgtcagc agcaccaagt ccggcgagcg tcgcagatgg acgaggcggc
     1261 gctggtttcg gcggtcacgc tgcccttgtg gtttccgccg gtacgcctgg aggacggtgc
     1321 cggtgaagcc agctacatgg atgccatgtt tgcgaccgat gcgaatatcg agcaggcgat
     1381 cgcagacggc gcggacgagg tgtgggtgat ctggaccgtt agtcagcgcg gcacagtccg
     1441 acccggcttt acccctgggt actttcaaat gatcgaggcg atcgccaact cccggatccg
     1501 cgcggtcctt gatcgcatcg agcgcaacaa cgccgcaatc gaggctggac aaccggggga
     1561 gtttcagcgc cggatcgagg tcaaatggct cag
//
LOCUS       DATAOZ010000372         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1202993, whole genome shotgun sequence.
ACCESSION   DATAOZ010000372 DATAOZ010000000
VERSION     DATAOZ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202993"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(459..1004)
                     /locus_tag="VHR64_09460"
     CDS             complement(459..1004)
                     /locus_tag="VHR64_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303096.1"
                     /translation="MDDDDRIDGKPVSHWHRPIGRRNVIAGIAGLGVAAGIGASQVLA
                     QDATPSATEEPSDTTIDEQPGKMAEIGAAYQDFVGKLATNLGESDTAKVDTAIRDALK
                     AMVDDQYTAGNISRNLADDLKSRIDSSDAPLAVAMLGGMGFRRVERRRDRRHDGQNDD
                     DTQPGDGDTTEQPSTESTPSI"
     gene            1197..1862
                     /locus_tag="VHR64_09465"
     CDS             1197..1862
                     /locus_tag="VHR64_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3303097.1"
                     /translation="MRILLVEDDEALADVTARGLRQAGLAVDLALDGEAAIENASVNA
                     YDVVVLDRQLPGIHGDDVCRALLRLETPPRILMLTAAGNLDDRVRGLQIGADDYLPKP
                     FAMAELVARIQALGRRPNAVRFPVIEVGDLQIDTNQRTARRGERDLNLTRKEFGVLEV
                     LAGKPGDVVSAEELLEKVWDEEADPFTNAVRITIMTLRRKLGEPQPIETVIGVGYRLL
                     EQP"
     gene            1859..3001
                     /locus_tag="VHR64_09470"
     CDS             1859..3001
                     /locus_tag="VHR64_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3303098.1"
                     /translation="MTPFRPSVRLKLTLLYGVLFVAAGLLLIAVTYGLVRRELAPNAL
                     QPGPRIERQHNQPANPDEREAGARREERRFALRQLWRQSLVALGLATVGALALGWLLA
                     GEVLHPIQRITAHAKSSSATTLDERINLDGPNDELKELADTFDAMLDRLQAAFRAQQG
                     FAAQASHELRTPLAIIRAEAEVAGAAPDATERERQSTGAILTAVGRSERLVDGLLALT
                     RSESTMLDAGPVDLADLTGDVVGEYVGEADEARVRLELTLDAAEVRGDPALLQRMVGN
                     LVQNAIRYNQPDGSVAVSVRPESGVARLTVENSGPVVSQEEIDQLFQPFVRGDWARRN
                     RSGFGLGLAIARSVATAHGGTIQAVPGPNGGLRITVDIPLLTAQSG"
     gene            complement(2961..>3194)
                     /locus_tag="VHR64_09475"
     CDS             complement(2961..>3194)
                     /locus_tag="VHR64_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303099.1"
                     /translation="HIEGNTGFEKELHPHATTTELVKAALTGRDPLMAPGILAVAALI
                     AIVASVAVSEERRPSHQPQVISRIAPSGAGYRR"
BASE COUNT          509 a         1063 c         1086 g          536 t
ORIGIN      
        1 agccgagcag gcgagcagct acggaaaata tagtgcgggc ttaccacgcc ccgcccggac
       61 cggatggcgg ccggatcgtt cggggaacca acaccggcca cgcttcggtt ccgtccatta
      121 cccgtgggga tgaacgatgc ggggcgtggt aaccccgcgc tacggttcgg tgcggttgct
      181 tgtgagccgg tgggttagca gaccaaggca tgtacggagc cacgccacta cggtacgaaa
      241 ggccggtcac gggggccggg gctacatccc tcataactcc gcgccttcgc gccttcgcgc
      301 cttcgcgccc caactgctcg cctgctcgcc tccagacaca gcgaaacgga ccggggctag
      361 atcacgtccg gtccgtttcg cggttgcgcc tttcggcgcc ggggaaaggg ggttgggcga
      421 tccgacccac gggaaggaga ggccggatca ggggcgggct agatggatgg ggtcgactcg
      481 gtgctgggtt gctcagtggt gtcgccgtcg cccggttgag tgtcatcgtc gttctggcca
      541 tcatgccggc ggtcccggcg acgctcgacg cggcggaaac ccatgccacc gagcatggca
      601 acggccagcg gggcatcgct cgagtcgatc cgcgacttca ggtcgtcggc cagattgcgc
      661 gagatgttgc cggcggtgta ctggtcatcg accatcgcct tgagcgcatc ccggatcgcc
      721 gtgtcgacct tggccgtatc gctctcgccg aggttcgtcg ccagcttgcc gacgaagtcc
      781 tggtaggccg cgccgatctc cgccatcttg cccggctgct catcgatggt ggtatcactc
      841 ggttcttcgg tggcggacgg cgtggcgtcc tgggccagca cctgcgatgc cccgatacca
      901 gccgcaacgc ccaggccggc gatgccggcg atcacgttgc gccgaccgat cgggcgatgc
      961 cagtgcgaga ccggcttgcc atcgatccgg tcgtcatcat ccacgcgtgg ttccatcatg
     1021 ctctccttgt cgcatccggc gcccggccgc gatgcaatcg actcgttcga ctatcagtcg
     1081 ttgatcacac cctatcgacc cggacgtatg gtccgcatat ggccgtgacc atatgaaaag
     1141 cacatctgac ttagatgaga atgaaccggg tttgaggcgg tatcgaggac acgacggtgc
     1201 gaatactcct tgtagaagat gacgaggccc tggctgatgt cactgcccgt ggtttgcggc
     1261 aggcgggcct cgccgtcgac ctcgcactgg acggcgaagc ggcgatcgag aacgcgtcgg
     1321 tcaatgccta cgacgtggtc gtgctcgatc ggcagcttcc cggtatccac ggtgacgacg
     1381 tctgccgcgc cctgctccgg ctcgagacac caccacgcat cctgatgctg acggctgcgg
     1441 gcaacctcga tgatcgcgtc cgcggtctcc agatcggtgc cgacgactac ctgccgaagc
     1501 ccttcgcgat ggccgagctg gtggcccgca tccaggcgct tggtcgccgg ccgaacgcgg
     1561 tccggtttcc ggtgatcgag gtcggtgact tgcagatcga caccaaccag cgcactgccc
     1621 ggcgcggcga gcgcgacctc aacctcactc gcaaggagtt cggcgtactg gaggtgttgg
     1681 ccgggaagcc gggagatgtg gtcagtgccg aggagctgct cgagaaggtc tgggacgagg
     1741 aggccgaccc cttcaccaac gcggtccgga tcacgatcat gacgcttcgc cgcaagctgg
     1801 gcgagccgca gccgatcgag acggtgatcg gcgtgggtta ccgcctgctg gagcagccgt
     1861 gaccccgttc cggccgtccg tgcggctcaa gctgacgctg ctatacggcg tgctgttcgt
     1921 ggctgccggt ctccttctga tcgccgtgac ctacgggctg gtccgccgcg agctggcgcc
     1981 gaacgcgctc cagccggggc cgcgcatcga gcggcagcac aatcagccgg ccaatccaga
     2041 cgagcgcgag gccggcgccc gtcgcgagga gcgccggttc gccctgcgcc agctttggcg
     2101 gcagtcgctg gtcgcgctcg ggctggcgac ggtcggcgca ctggcgttgg gctggctcct
     2161 ggccggtgag gtgctgcatc cgattcagcg gatcaccgcg catgccaagt cgtcgtccgc
     2221 gacaacactc gatgagcgga tcaatctcga tggtccgaac gatgagttga aggagctggc
     2281 cgataccttc gacgccatgc tcgaccggtt gcaggctgcc ttccgcgccc agcagggttt
     2341 cgccgcccaa gcctcccacg agttacggac accgcttgcc atcatccggg ccgaggcaga
     2401 agttgccggc gccgccccgg acgcaaccga gcgggagcgg caaagcactg gggcgatcct
     2461 gaccgcggtc gggcgcagcg agcggctggt cgacggcctg ctggccctga cccgtagcga
     2521 gagcacgatg ctcgacgccg gaccggttga cttggccgat ctgaccggcg atgtcgtcgg
     2581 cgagtacgtc ggagaggccg atgaggcccg ggtccggctc gaactcacgc tcgatgccgc
     2641 cgaggtccgc ggcgatccgg ctctgctgca gcggatggtc ggcaacttgg tccagaacgc
     2701 gatccgctac aaccagccgg acgggagcgt ggcggtatca gtccggccgg agagcggagt
     2761 ggcccggctg acggtcgaga acagcggccc ggtggtcagc caggaggaga tcgaccagct
     2821 cttccagcca ttcgtgcggg gcgactgggc gcgccgcaac cggagcggct tcggtctcgg
     2881 cctggcgatc gcccgctccg tcgccaccgc ccacggcggc acaatccagg ccgttcccgg
     2941 accgaacggc ggcctgcgga tcaccgtcga tatcccgctc ctgacggcgc aatccggctg
     3001 atcacctgcg gctggtgcga aggccgccgc tcctccgaaa ccgcaaccga cgccacgatc
     3061 gcgatcagcg ccgcgacggc gaggatgccg ggcgccatca atggatcccg accggtcagc
     3121 gccgccttga ccagctcggt ggtcgtggcg tgtgggtgca gctccttctc gaagccggta
     3181 ttgccctcaa tatg
//
LOCUS       DATAOZ010000373         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8042811, whole genome shotgun sequence.
ACCESSION   DATAOZ010000373 DATAOZ010000000
VERSION     DATAOZ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8042811"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VHR64_09480"
     CDS             <1..470
                     /locus_tag="VHR64_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX3303100.1"
                     /translation="QTNLLALNATIEAARAGDAGRGFAVVAQEVKSLASQTARATDDI
                     TAKITAIQQATRQTVESNASIQGTVEEVQTSADRIREAMELQAQTVTMITAAVDETAL
                     AADSMSSTIAAIRSDTENVAKDIDQVEQGFGRFSDQISNFKSSAKAFVSGMAA"
     gene            541..1245
                     /locus_tag="VHR64_09485"
     CDS             541..1245
                     /locus_tag="VHR64_09485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3303101.1"
                     /translation="MNILITGASRGIGAAAYTLLKKHAQNVVGHSTRGSDILIAGDLS
                     DPAAPRDIWDTAMDELGGHVDVLVNNAGVYEAVPDNAPDDEWHAAWARTMTINLQAAA
                     DLSRLAVSHFLDRGGGGRIINVASRAAYRGDSPQHWHYAASKAGMVGMTKSIARGYAS
                     EGVLCFAVAPGFTVSEMTAEYLTGRGGEQILADIPLGRVASTDEVAEVIRWLATEAPA
                     SATGTVVDVNGASYVR"
     gene            1235..>1620
                     /locus_tag="VHR64_09490"
     CDS             1235..>1620
                     /locus_tag="VHR64_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3303102.1"
                     /translation="MFASLLGPVMLAAQQIVIPFGHPKLIEQPRAPIQTSGPLWVARC
                     NGSDDWTKPAPPVRIHANTYLVGTCGIASILIVGDQGDILIDGGPEEAAPLIAANIDR
                     LGFRVEDIRYILSSHEHHDHIGGIAEL"
BASE COUNT          303 a          543 c          513 g          261 t
ORIGIN      
        1 gccagacgaa tctactagcc ctcaacgcga ccatcgaggc cgcgcgcgcg ggcgatgccg
       61 gtcgcggctt tgccgttgtc gcccaggaag tgaagagcct cgcctcgcag actgctcgcg
      121 ccacggacga catcacggcg aagattaccg ccatccagca ggcaacccgt caaacggtcg
      181 agtcgaacgc gtccatccag ggcacggtcg aagaagtcca aacctccgcc gaccggatcc
      241 gcgaagcaat ggaattgcag gcgcagacag tcaccatgat tactgccgct gtcgacgaga
      301 ccgcgcttgc agccgactcg atgagctcga ccattgccgc gatccgcagc gacacggaga
      361 atgtggccaa ggacatcgac caggtcgagc agggcttcgg tcgcttcagc gatcagatct
      421 ccaacttcaa gtcgagcgcc aaggcgttcg tcagcgggat ggccgcctaa gccttaccgg
      481 cgaagcagtc cggcgtcgcg ccggattgct tcgctgcgct cgcgatgaca gagcgggcgc
      541 atgaacatcc tcatcaccgg tgcaagccgc gggatcggcg ccgcggccta cacgctcctc
      601 aagaagcatg cccagaatgt ggtcggccac tcgactcggg gcagtgacat cctgattgcc
      661 ggtgatctct cggaccccgc cgccccgcgc gacatctggg acaccgcgat ggacgagctc
      721 ggcggccacg tcgacgtgtt ggtgaacaac gccggcgtct atgaggccgt gccggacaac
      781 gcgccggacg atgaatggca tgccgcctgg gcgcggacga tgaccatcaa cctccaggca
      841 gcggcggacc tgtcgcggct tgcggtgtcg cacttccttg atcgcggcgg cggtggccgg
      901 atcattaatg tcgcgagccg cgctgcctat cgtggcgaca gccctcagca ctggcattac
      961 gcggcgtcga aggcggggat ggtcggcatg accaagagca tcgcacgcgg ctacgcgagc
     1021 gaaggagtcc tgtgcttcgc cgtcgcgccg ggcttcaccg tgtccgaaat gaccgccgaa
     1081 tatctcactg gccggggcgg ggagcagatc ctggcggaca tcccccttgg ccgcgtcgcg
     1141 tcgaccgacg aagtggccga ggtcatccgc tggctggcga ccgaggcccc tgcatcggcc
     1201 accggaaccg tcgtggacgt gaacggcgcc agctatgttc gctagcctgc ttggccccgt
     1261 gatgctcgcg gcccagcaga tcgttattcc gttcggtcat cccaagctta tcgagcagcc
     1321 gagggcgccg atacagacgt cgggtccgct gtgggtcgcc aggtgcaacg ggtcggacga
     1381 ctggactaag cctgcaccgc ccgtccgcat tcatgccaac acgtatctgg tcggcacctg
     1441 tggcatcgca tcgatcctga ttgtcggcga ccagggcgac atcctgatcg atggtggacc
     1501 ggaggaagcc gctccgctca tcgccgccaa catcgaccga ctaggcttcc gcgtcgagga
     1561 tattcgctac atcctcagca gccacgagca ccacgatcac atcggcggca tcgccgagct
//
LOCUS       DATAOZ010000374         6182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7257100, whole genome shotgun sequence.
ACCESSION   DATAOZ010000374 DATAOZ010000000
VERSION     DATAOZ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7257100"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1109)
                     /locus_tag="VHR64_09495"
     CDS             complement(114..1109)
                     /locus_tag="VHR64_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3303103.1"
                     /translation="MARHPWVAAADEGIRWGVQLIVPNERNALANLITTAGNVEELGY
                     DFLSIFDHPLMHVDPWIALSGLATRTSKVRLGSTVNCAMYRHPAHLARLATDLDNLSN
                     GRHILGLGSGWLRPEFSALGREFGSAGHRLAALEDTLAIVHGVWSSPPFNYQGTVFST
                     EGMVAEPPPVQQPHPPIVVAGNGERVTLRKVAEWGDACNVREEISLTDDTIDDQTRAG
                     AVRKMLDALAGHCETLDRDPSEVLRTHFTLYLILGETMAEARAKADALDTSKSTSPGT
                     RRDGKTAMLIADPDRAIRYYQAMVDIGIQYFIVQLDARDQQTIELLKTKVAPRVG"
     gene            1271..1543
                     /locus_tag="VHR64_09500"
     CDS             1271..1543
                     /locus_tag="VHR64_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303104.1"
                     /translation="MEQSRTIRIDQYVEDVTSPVVREILTHLCEMENPSYGSIIEWCE
                     TRGDCAQAIVCPECGAQYLLDDEEMAELRVISARTGEILSCGVIWD"
     gene            1571..1984
                     /locus_tag="VHR64_09505"
     CDS             1571..1984
                     /locus_tag="VHR64_09505"
                     /inference="COORDINATES: protein motif:HMM:NF045421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303105.1"
                     /translation="MADDGGDGTRANPWRLKTPPGTSEYEMYRDESANPPTLVCVVGK
                     TTLGYQLRCIEDLHTMLREHGDWVPLGAADEQKSAAEGTVEAWGRSADNPVGGWYGTK
                     KGLRGRFGMYVPPLLERLGLAELEHNPRNNRMRAK"
     gene            complement(2199..3815)
                     /locus_tag="VHR64_09510"
     CDS             complement(2199..3815)
                     /locus_tag="VHR64_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX3303106.1"
                     /translation="MLSVSNLSRSFGGRTILDSISFVINPGDRIGLIGPNGAGKSTLL
                     RMIAGFDRPDRGSVSISPGERIGMLRQGFADLADGTLADLLDGPTNGLASASRNLEQT
                     LARSDGSDEWLVEYDAAQSHFDALGGYSAVAELETLLGAFGLENRAWDTPLTELSGGE
                     KTRAGLAALIVSRPDYLLLDEPTNHLDAEGQRFLAQLFSEFDGGVLIVSHDRHFLDEV
                     VTGLLVLDDQSEGIEQFTGTYSDYRDAKAREAELLAGAYRRQQEEIARIRGDIRAVGT
                     HALATENATTNDYLRGRSKKVARTAKVRERKLERMLESADLIDKPERRWTMAAEFAPA
                     AESGRDVALLTGVSVQYSDSSILREVDLLIRAGDRIAVIGPNGAGKTTLLKLIAGELE
                     PTHGTVRVGANVRVGWFAQEQETLPFALTPVQLVRLRSELSESEARTFLHRFLFTAAN
                     VTNPVATLSYGERSRLALALLVLDGCNLLLLDEPLNHLDITSREQFEVALSEFTGTLL
                     IVLHDEFAADRICNRRLAVKDGSVTEETYL"
     gene            4305..5366
                     /locus_tag="VHR64_09515"
     CDS             4305..5366
                     /locus_tag="VHR64_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3303107.1"
                     /translation="MSKHNVLKSAMTRRQALRGAAGIGLGAIAAPYFGGSGAIAQDVS
                     CPPPATPAANVGTPEAMSGKKIGVSVAYLSVPFYANFKTGLADGAKRFGFEYELRDGG
                     GGDLAKEVAVLQDFIAKKVDLILLTPSGEGIIPGIKQVNDAGIPVIEVNNRAGFQSGQ
                     ADVVTYVGADDVEFGRLQGKLLIDTLAGAPAKVGYEQGISGTSPQILRGQGFNEAIKD
                     HPEIEVVATVTNDFSSSKALAVTQDLLTRFPKGSLDFIVMQGPDGVPSAQFARENGRE
                     EVRFILGDYPADVRQAIIDGYIFGTINQDPYPQAFEAMHMAWLYFNGRESEIPKPYFL
                     DLPIITKENAEATPPAWGC"
     gene            5409..>6182
                     /locus_tag="VHR64_09520"
     CDS             5409..>6182
                     /locus_tag="VHR64_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303108.1"
                     /translation="MPTFAMKVYDLQWFTTFGMDVDQAAEALAEDGIDVVLTQNHIDP
                     LPTSGVDQHEYLARYQDRIRAYDDRAWVDALKRHGLTVLETSAVLFDPPALLRFPDAR
                     PVDANGEPHVGLDWYVGICPTHDAYLDEKVTKLARVVEELSPDGLFLQFIRYPGFWEN
                     WTWSPEYQFSDSDRYCFCDRCRSRFSEALHLSLPPGDAASQAHLILRHHGAEWTDWRC
                     RVIADIVRRSREIGMTNPDGTQLLLNTLPFPATDFGNQAV"
BASE COUNT         1178 a         1897 c         1966 g         1141 t
ORIGIN      
        1 tggtcatcag ggaccaacga ctatacggac cgcatcgccg attggttgtg ttgccgggca
       61 aagaagaatc tgcctccacc gcggcccaaa tccgctccgg tcgccatggg cgctcacccc
      121 acgcggggcg ccaccttcgt cttgagcagc tcgatcgtct gctgatcgcg ggcgtcgagc
      181 tgaacgatga agtactggat gccgatgtcg accattgcct gatagtagcg gatcgcgcgg
      241 tctggatcag cgatcaacat cgcggttttg ccgtcccgcc gggttcccgg tgaggtggat
      301 ttcgaggtgt cgagcgcatc ggccttcgct ctggcctcgg ccatggtctc gccgaggatc
      361 aggtagaggg tgaagtgggt gcgcaggacc tcggacggat cgcgatcgag cgtctcgcaa
      421 tgtccggcga gcgcgtcgag catcttgcgc acggcgccgg cgcgggtctg gtcgtcgatc
      481 gtgtcgtcgg tgagggagat ctcctctcgc acgttgcagg catcgcccca ttccgcgacc
      541 ttgcggaggg tgacccgctc gccattgccg gcgaccacga tcggcgggtg cggctgctgg
      601 accggcggtg gctcggcgac catgccctcg gtgctgaaga cggtgccctg atagttgaac
      661 ggcgggcttg accagacacc gtggacgatc gcgagcgtgt cctcgagcgc ggcaaggcga
      721 tggccagcgc tgccgaattc ccgcccaagc gcactgaact ccggccgcag ccagccagag
      781 ccgaggccga ggatgtggcg gccattgctg aggttgtcga gatcggttgc cagcctggcg
      841 aggtgggcgg gatgccgata catcgcgcag ttgacggttg aaccgagccg caccttcgat
      901 gtccgcgtcg cgaggccgga aagggcgatc caggggtcga cgtgcatcag cgggtgatcg
      961 aagatcgaga ggaagtcgta gccgagctcc tcgacattcc cggccgtcgt gatcaggttg
     1021 gcgagcgcat tccgctcatt cgggacgatc agctgcacgc cccatctgat cccctcgtcc
     1081 gccgccgcta cccatggatg tcgcgccaca gaacactcct gcctgctgaa atcgtcgctg
     1141 cggggcattg tcgcgtatgc ccaggacatt cgggccgggt agtcggaggg attgaattaa
     1201 ttctttgcgg cagataccat cgcaacttca cacagcggca gccgccacag attttggagg
     1261 gggaatcgca gtggaacaat ccagaacaat ccgaatcgac caatacgtgg aagatgtgac
     1321 cagcccggtc gtccgcgaga tcctgacgca tctctgcgag atggagaacc cgagctacgg
     1381 ttcgatcatc gagtggtgcg aaacccgggg agactgcgca caggcgatcg tctgccccga
     1441 atgcggcgcg caatacctgc tcgacgacga ggagatggcc gagctgcgcg ttatctcagc
     1501 ccgaaccggc gagatcctct cctgcggcgt catctgggat tgacatgcca gacaggttgg
     1561 gagcgttggg atggccgatg acggcggtga tggcacgcgc gcgaatccat ggcggttgaa
     1621 aaccccgcct gggacatccg agtacgagat gtaccgcgat gagagcgcca atccgccgac
     1681 gctggtttgc gtggttggca agaccacgct tggctatcaa ctccgatgta tcgaggacct
     1741 gcacaccatg ctgagggagc atggcgactg ggtaccgctc ggcgctgccg acgagcagaa
     1801 atcggcggcc gagggcacgg tcgaggcgtg gggacgatca gccgataacc cggtcggtgg
     1861 ctggtacggc acgaagaagg gcctgcgggg ccggttcggc atgtatgtgc cgccacttct
     1921 ggagcggctg ggcctggccg agctggagca caatccgcgc aacaatcgta tgcgggcgaa
     1981 gtaggcttcg gggacgagcg tgccggtcag gtgggtgcat cgccaatact gatgtagggg
     2041 tttgccgtgg caaacccata taacccgatc ccatgatcgg taccaacccc gggtttagcg
     2101 ccgatcccgc atcccctatc aggctgcgtt cgatacggca acgggcggat ttgccacggc
     2161 aaatccctac aatcttcttc gctccttaac tgctcgcctc acaaatacgt ctcctcggtc
     2221 acactgccgt ccttgaccgc cagccgccgg ttgcagatgc ggtcggccgc gaactcgtcg
     2281 tggaggacga tcaggagcgt gccggtgaac tcgctgagcg cgacctcgaa ctgctctcgc
     2341 gatgtgatgt cgaggtgatt gaggggctcg tcgagcagca gcaggttgca gccgtcgagc
     2401 acgagcaggg ccagggccag gcgggagcgc tctccatagg agagcgtcgc aaccgggttc
     2461 gtgacgttgg cagcagtgaa cagaaaacga tgcaggaagg tccgcgcctc cgactccgat
     2521 agctcggatc gcagccgcac gagctggacc ggcgtcaacg caaacgggag cgtctcctgc
     2581 tcttgcgcga accagccgac gcggacgttt gcgccgacgc ggaccgttcc gtgggttggc
     2641 tccagctcgc ccgcgatcag cttcaggagc gtcgtctttc cggcaccgtt cggcccaatt
     2701 acggcaatcc ggtcacctgc gcggatgagt agatcgacct cgcgcaggat cgacgagtca
     2761 ctgtattgca cggagacgcc agtcaacagc gcgacgtccc ggcccgattc ggcagccggc
     2821 gcgaactcgg cggccatcgt ccagcggcgc tccggcttgt cgatcagatc ggccgactcg
     2881 agcatccgct ccagcttgcg ctcgcggacc ttggccgtgc gggcgacttt cttcgagcga
     2941 ccgcgcaggt agtcgttggt ggtcgcattt tcagtggcaa gggcatgggt gccgaccgcg
     3001 cggatatcgc cacggatgcg agcgatttct tcttgctggc gtcgataagc acccgccaac
     3061 agctcggcct cgcgcgcttt cgcgtcccga tagtcggagt aggtgccggt gaattgctcg
     3121 atgccctcgc tctggtcatc gaggaccagc agaccggtca caacctcgtc gaggaagtgg
     3181 cgatcgtgcg agacgatgag cacgccgccg tcaaactcgc tgaagagctg agcgaggaag
     3241 cgctggccct cggcgtcgag gtggttggtc ggctcatcca gcaacaggta gtcgggccgg
     3301 gagacgatca acgcggccag accagcccgg gtcttctcgc cgcccgaaag ctcagtgagc
     3361 ggcgtatccc atgcccggtt ttcgagcccg aatgcgccga gtaatgtctc caactcggcc
     3421 acggccgagt acccgccgag cgcgtcgaag tgcgattgcg ccgcgtcgta ctcgaccagc
     3481 cactcgtcgg atccatcgct gcgggccagg gtctgctcca ggttgcggga ggcagaggcg
     3541 aggccgttcg tcgggccatc gaggaggtca gcaagggtgc cgtcggcgag gtcggcgaaa
     3601 ccctggcgga gcatgccgat ccgctcaccg ggcgagatgg agaccgagcc gcgatcgggc
     3661 cggtcgaacc cggcgatcat gcggagcagg gtcgacttac cggcgccgtt gggaccgatc
     3721 agtccgatcc ggtcgcccgg gttgatcacg aatgagatgg aatcgaggat ggtgcggccg
     3781 ccaaacgacc gtgacagatt actgacggaa agcatgctaa cccctgtgac ccggcgcgtt
     3841 gcgggtcgat caatcgaatt cccgattggg ggttagtccg tcattctgcc ctcctgaggc
     3901 atcgcggctc atcgcacaag ccgggtacgc agtcaactat accgaagcag gcagccgctg
     3961 tcttccgcct ttcgacggag ttgtttcgcg ttctcacgaa ctccggcgga aaagagggcg
     4021 tgagctccac cccctgtggg ccatcttgcc gatccgcatg gcgcacccgc aggcgccccg
     4081 agaacatgag cttcgccgga gtgagcgacg ggaggtaccg cagtggaccg tctctcgtcc
     4141 cttaccggaa aatgtgctcc ctggtcaccg caatttgttg acacgcataa atgtcagtgg
     4201 gagaatccgc cccagttgga caactcatac tctgctgcgc gacatgtatc gctctcggca
     4261 ctcggcatct tttgcgatct gatgtgacgg aagggactgc gaccatgagc aagcacaacg
     4321 ttttgaagtc ggcaatgacc cgtcggcaag cgctacgggg tgcggccggc atcggacttg
     4381 gtgcgatagc cgcgccatat ttcggaggca gtggcgcaat agcccaggat gtatcttgtc
     4441 cgcctcctgc cacgccggcc gcgaatgtcg gcacgccaga ggcgatgagc ggcaagaaga
     4501 tcggcgtctc ggtcgcttac ctctcagttc ccttctacgc caacttcaag actggcctcg
     4561 ctgacggcgc gaaacgattc ggcttcgaat atgagctgcg agacggcggc gggggagatc
     4621 tcgccaagga ggtagccgtc ctccaggact tcatcgcaaa gaaggtcgac ctgatcctcc
     4681 tcacgccatc cggagaaggg atcatcccgg ggatcaagca ggtgaacgat gccggcattc
     4741 cggtaatcga ggtcaacaac cgcgccggct ttcaaagtgg tcaggctgat gtcgtcacct
     4801 atgttggagc ggacgacgtg gagttcggcc gcctgcaggg caagctgctg atcgatacgc
     4861 tcgctggcgc accagcaaag gtgggctacg agcaggggat tagcggaacg tcgcctcaaa
     4921 tcctgcgcgg ccaagggttc aatgaggcaa tcaaggacca tccggaaatc gaagtggtcg
     4981 cgaccgtaac aaatgatttc agctcttcga aggccctggc ggtcacccag gacctactga
     5041 cccgcttccc gaagggcagc ctcgatttca tcgtgatgca gggccccgat ggcgtcccct
     5101 cagcccagtt cgcccgcgag aatgggcgcg aagaggtcag gttcattctt ggcgactacc
     5161 cggcggatgt ccgccaggcc attatcgacg gctacatctt cggcacgatc aaccaggatc
     5221 cgtacccgca ggcattcgag gcaatgcata tggcctggct gtatttcaac ggacgcgaga
     5281 gcgagatccc gaaaccctac ttcctcgatc tcccgatcat caccaaggag aatgctgaag
     5341 ccacgccacc tgcctggggc tgctagctcg agcgttcatt cgtcttcagt acagcgagcg
     5401 tcgagacaat gccgaccttc gcaatgaagg tctacgacct gcaatggttt acgacctttg
     5461 gcatggacgt tgaccaggcc gccgaagcac ttgcggagga tgggatcgat gtggttctca
     5521 ctcagaacca catcgatcca ctgccaacgt caggcgtgga tcaacacgag tacctggctc
     5581 gctatcaaga ccgtattcgt gcctatgacg atcgggcgtg ggtcgatgcc ctaaagcgtc
     5641 atggactcac cgttctcgag acatcggccg tcctcttcga tccaccagcg ctcctaaggt
     5701 tccccgacgc ccgcccggtc gatgccaacg gagagccgca cgtgggcctc gactggtacg
     5761 tcggcatctg tcccacgcac gatgcttatc tggacgaaaa ggtgacgaag ctcgctcgtg
     5821 tcgtcgagga gttgtcgccc gacggtctct ttctgcaatt catccgctac cccggatttt
     5881 gggagaactg gacgtggtca ccggaatacc agttctccga ttccgatcga tactgctttt
     5941 gcgatcgttg ccgaagccgc ttttccgaag ccttgcacct ctccctccca ccgggagacg
     6001 cagccagcca agcgcacctc atcctgcggc accacggcgc tgaatggacg gactggcgat
     6061 gcagggtcat tgcagatatc gtgcgtcgct ctcgggaaat cggcatgaca aatcccgatg
     6121 gcactcagct cctgttgaat acgttgcctt tcccggcgac cgattttggc aaccaggccg
     6181 tg
//
LOCUS       DATAOZ010000375         7281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6991707, whole genome shotgun sequence.
ACCESSION   DATAOZ010000375 DATAOZ010000000
VERSION     DATAOZ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6991707"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..248)
                     /locus_tag="VHR64_09525"
     CDS             complement(45..248)
                     /locus_tag="VHR64_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303109.1"
                     /translation="MTDAPFLAEPTEEDVAAIAAVLFSEQQKTIQESAAPSPWVIAAR
                     REGVDKWEQPTGLSRLRDLRVRE"
     gene            complement(245..1831)
                     /locus_tag="VHR64_09530"
     CDS             complement(245..1831)
                     /locus_tag="VHR64_09530"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX3303110.1"
                     /translation="MSIEQSDRTEVKPAPQHLAPAERYQALRDEADSQHAAADAKQRE
                     RGKRGARERVLALLDPDSFVELDPFVQHRADNFGMGEKRPYGDGVVTGLGTIDGRKVA
                     IFSQDFTVFGGSLGEAFAEKVVKIMDLAEHYGCPIIGINDSAGARIQEGVEGLNGYGK
                     VFVRNSRLSGVVPQISIIAGPCAGGAVYSPAMTDVTIVVAGVGQMFITGPDVIKTVTG
                     ETVTHEELGGADTHFRISGVAHMVADDEDALVDLVRDLLAHLPSNNLEEPPRIPNAEG
                     ELRCEALNTLLPDESTKAYDMHEVLAEVLDDGDFLELQGAHAGNILTGFGRIAGRTVG
                     IVANQPAVLAGTLDIDASVKGGRFVRLCDAFNVPLIVFEDVPGFLPGTEQEYRGIIRH
                     GSKLLYAFGEATVPKITVITRKAYGGAYVVMNSKAIGADFVYAWPTAEIAVMGSEAAV
                     PLLFRRDLAAADDPVELKRELIAEYQEMFASPYQAASRGYVDDIIEPAETRNHLIRAL
                     ALSETKRVSVPRRKHGNIPL"
     gene            complement(1812..3380)
                     /locus_tag="VHR64_09535"
     CDS             complement(1812..3380)
                     /locus_tag="VHR64_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX3303111.1"
                     /translation="MATTDKSIATDSDIEIKPVYRPEDGDDREDPGVFPYTRGIYPTM
                     YRGRRWTMRQYAGFSTAAESNCRYRLLLERGQTGLSVAFDLPTQLGMDSDDPRAEGEV
                     GRVGVPISTLDDMRALFDGIQQDKVTTSMTINAPASLLLLMYEIVAEEKGADPRKLGG
                     TIQNDILKEYAARGTFIFPPRPSMRLVTDTFAYCAEHLPNWNTISISGYHMREAGCSA
                     AQEIAFTLANAIAYLRAAEQTGLGVDQVAPRMSFFFACHSNFFEEVAKFRAARRLWAL
                     ITKEQFDATDPKSWTMRFHTQTGGVTLTAQQPLNNIVRVAYQALSAVLGGTQSLHTNS
                     FDEALGLPTEESATVALRTQQILADETGVPDTADPLAGSYFVERLTDELEAHAREWLD
                     QIEHEGGAVTAIERGFVQNAIADNAYRREQAIDRGEAIIVGVNRYADGREPDIPIQPL
                     DEDAIAEQRRRTAEYRARQDRGAVEAALADVKTAAEGDTNLLPVMRQALVGGATLGEI
                     CTVLRGVFGEHRAI"
     gene            complement(4361..4825)
                     /gene="mce"
                     /locus_tag="VHR64_09540"
     CDS             complement(4361..4825)
                     /gene="mce"
                     /locus_tag="VHR64_09540"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX3303112.1"
                     /translation="MTVSELLTHGRLHHVGIVVADLDAAIATYQALGFGEADRFDIPA
                     QGVRAAFFPLAQGSVELIQPTDSEGAIGRFMAKRGEGFHHVAYQVDDLATTLVELAAA
                     GIELIDFAPRVGGHGLWIAFIHPRACNGVLTELVQEPTSREVEESSSREAKS"
     gene            complement(4822..>7281)
                     /locus_tag="VHR64_09545"
     CDS             complement(4822..>7281)
                     /locus_tag="VHR64_09545"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX3303113.1"
                     /translation="KQANRRVDQLFLTRRALLMKSAVIGGFSILLTRLGYMQIGKGKD
                     YQAAATTNITDWRELKPSRGVIVDRKGRLMAKNRKSWSVSVIPADIRQLESTQLAYVR
                     EQLMTALRLPDVVMINPGSIPAEAKDQVYRRVGRLLGDTTEQDFENTKNWIKAQLRFN
                     DYAKFDDISADTAAQFNTYQADLPGVEIVNFFDFVIQNYRFIETPYLLKTDISREVAM
                     KLAANQLYLPGVVLSDEVLTRQYPGGPTMSHILGFAGPVNSEELNDDSNVSSRDEDGN
                     PQYRFYKPGDIIGKQGLEYFYEEQLRGGKGGYLYERDANGRELRRLTEGAQPAVPGRN
                     LQLTVDVELQAAVSRILQQELPLAMERRQEADAKANLPYKEHTTQGGAVVIFDVNTGE
                     VLAMASYPTYDNTLFVEGISERKYAELRDDPRGPLYDKTYMQKYPPGSVIKPFMALTG
                     MREKVIDANTQFFCGGGIKVPYDFDESKGNIYGCWAKAGHGAMAVEKAIEQSCDVFFY
                     NVGVSAGQRRQENGKVNHYFDFSLASQVQGEEHSFSGLGINKIHAALTKRFWFGRITG
                     IDLPVETAGLVGSVKWLSDNYEGAGWSIGDTVNASIGQGYIEFSPLQLAMNTAALCNG
                     GNVYRPRIVHSVVDADGNVLQEFAPVIKRKIKFNQDHLTTVLNGMHDVVHNLSGTAFM
                     NADGTRKFSHTNPEGEEEIQLGGKTGTAEFQKGVDAAGDPVYDAHAWFSMFAPFDKPE
                     IAIAVFIESGGEGSTNAVPIADKAARAYFELTGRRKRGMILREDGEPVGDKVVSPLDD
                     PNAGKLKSSTPAATPEANPGQ"
BASE COUNT         1207 a         2249 c         2453 g         1372 t
ORIGIN      
        1 cccgatcctg atccccagat cccgtgcccg cttccgctgc tctgtcattc tctcaccctt
       61 aagtcacgca atcgggagag cccggttggc tgctcccact tgtctactcc ctcgcgccgg
      121 gcggcaatca cccatggcga aggagcggct gattcctgaa tcgttttctg ctgctcgctg
      181 aagaggacgg ccgcgatggc tgccacatcc tcttcggtcg gttcagcgag gaacggggcg
      241 tcggtcacag cgggatattc ccgtgcttcc ggcgcgggac gctgacccgc ttggtctcag
      301 agagagccag cgcgcgaatc aggtggttgc gagtctcggc cggctcgatg atgtcgtcga
      361 cgtacccgcg cgaggccgcc tggtatgggc tggcgaacat ctcctggtac tcggcgatca
      421 gctcacgctt gagctcgacc gggtcgtcgg ccgcggccag atcgcgccgg aagagcaacg
      481 gaacggccgc ctcggaaccc atcacggcaa tctcggcggt cggccaggca tagacgaaat
      541 cggcgccgat cgccttcgag ttcatgacga cgtaggcgcc gccgtaggcc ttgcgcgtga
      601 tgacggtgat cttggggacg gtcgcctcgc caaaggcgta gagcagcttc gagccgtggc
      661 ggatgatgcc gcggtattcc tgttcggtgc caggaaggaa gccaggcacg tcctcgaaga
      721 cgatcagcgg gacgttgaag gcgtcgcaaa gtcgcacgaa gcggccgccc ttcacgctgg
      781 cgtcgatgtc gagcgtcccg gccagcaccg ctggctggtt ggcgacgatt ccaacggttc
      841 gcccggcgat gcggccgaac ccggtcagga tattgccggc gtgcgcgccc tgcaattcga
      901 ggaaatcgcc gtcgtcgagc acctcggcca ggacctcgtg catgtcgtag gccttcgtgc
      961 tctcgtcagg gaggagcgtg ttcaacgcct cgcaacggag ctcgccttcg gcattcggaa
     1021 tgcgtggtgg ctcctcgagg ttgtttgagg gcaggtgggc gagcaggtcg cggaccagat
     1081 cgaccagcgc gtcctcgtcg tcggcgacca tgtgggcgac gccgctgatc cggaaatggg
     1141 tatcggctcc gcccagctcc tcgtgcgtga ccgtctcgcc ggtgacggtc ttgatcacgt
     1201 ccgggccggt gatgaacatc tggccgacgc cggcgacgac gattgtgaca tcggtcatgg
     1261 ccggggaata gaccgcacct cccgcgcagg ggccggcaat gatcgagatc tgcgggacca
     1321 cgccggagag ccgggagttg cggacgaaga ccttgccgta gccgttcagc ccctcgacgc
     1381 cctcctggat gcgagcgccg gccgagtcgt tgatgccgat gatcgggcag ccgtagtgct
     1441 cggccagatc cataatcttg acgaccttct cggcgaaggc ctcacccagc gatccgccga
     1501 agaccgtgaa atcctgcgag aagatcgcga ccttgcggcc gtcgatggtg cccagaccgg
     1561 tcacgacgcc gtcgccatac ggccgcttct cgcccatccc gaaattgtcg gcccggtgct
     1621 ggacgaaggg atcgagctcg acgaatgagt ccgggtcgag cagagctagg acccgctcgc
     1681 gcgcgcctcg cttgccccgc tcgcgctgtt tggcatcggc ggcggcatgc tgggagtcgg
     1741 cctcatcgcg gagcgcttgg taccgctcgg ccggcgccag gtgctgggga gcgggcttta
     1801 cttccgtccg gtcagattgc tcgatgctca ccgaagactc cccgcagtac cgtgcaaatc
     1861 tcgccgagtg tcgccccacc aaccaatgct tgcctcatga ccggcagcag gttggtgtcg
     1921 ccctccgcgg ccgtcttgac atccgcaagc gccgcttcga ccgcgccccg gtcctgccgc
     1981 gcacgatact cggcggtccg gcggcgctgc tcggcgatcg cgtcttcgtc gagcggctgg
     2041 atcgggatat ccggctcgcg gccgtcggcg tagcggttga ccccgacgat gatcgcttcg
     2101 ccccgatcga tcgcctgttc gcgccggtaa gcattgtcgg cgatcgcatt ctggacaaag
     2161 ccgcgctcga tcgccgtgac cgcgcctccc tcgtgctcga tctggtcgag ccactcgcgg
     2221 gcgtgtgcct cgagctcgtc ggtcagccgc tcgacgaaat atgaaccggc gagcggatcg
     2281 gccgtgtcgg gcacaccagt ctcgtcggcc aggatctgct gcgtgcggag ggcaacggtc
     2341 gcgctctcct cggtcggcag gccgagcgcc tcatcgaagc tgttggtgtg caggctctgg
     2401 gtgccgccaa ggaccgccga gagcgcctgg taggcgacgc ggacgatgtt gttgagcggc
     2461 tgctgggcgg tcagggtgac gccaccggtc tgggtgtgga agcgcatcgt ccacgacttc
     2521 ggatcggtcg cgtcgaactg ctccttcgtg atcagagccc agagccgccg cgccgcccgg
     2581 aacttggcga cctcttcaaa gaagttgctg tggcaggcga agaagaagct cattcgcggc
     2641 gcgacctgat cgacgccgag ccccgtttgc tccgctgccc gcaggtaggc gatcgcattg
     2701 gcgagggtga aggcgatctc ctgcgcggcc gaacatcccg cctcgcgcat gtgatagccg
     2761 gagatcgaga tcgtgttcca gttcggaaga tgctcggcgc agtaggcgaa ggtgtcggtc
     2821 acgagccgca tcgatggccg cggcgggaag atgaaggtgc cgcgcgcggc gtactccttg
     2881 aggatgtcgt tctggatcgt gccgccgagt ttgcgcggat cggcaccctt ctcctcggcc
     2941 acgatctcgt acatcagcag gagcagcgag gccggcgcgt tgatcgtcat cgacgtcgtg
     3001 accttgtcct gctggatccc gtcgaagagc gcccgcatat cgtccagcgt gctgatcggc
     3061 acgccgacac gaccgacttc accctcggcg cggggatcgt cagagtccat gccgagctgg
     3121 gtcgggagat cgaaagcgac gcttaggccg gtctggccac gttcgagcag gaggcgataa
     3181 cggcagttgc tctcggccgc ggtcgagaat ccggcgtact ggcgcatcgt ccagcggcgg
     3241 ccccgataca tagtcggata gatgccgcgg gtgtagggga agacgccggg atcctcgcgg
     3301 tcgtcgccgt cttcgggccg gtagaccggt ttgatctcga tatcggaatc ggtcgcgatg
     3361 ctcttgtcgg tcgtagccaa tcccgtaccc tcgccgcgcg aaacggtcac gcggccccag
     3421 cggagatggt accagccaga gcgagtcatc gccgcggccg cgtggtggcg aggctccgca
     3481 ctcgccttgt ctcgccgtcg gttcgttcgt ggtgcaggcg agtggtcgtt cggcgccacc
     3541 cggggataaa tcccggggct gattcaataa gccagctgaa gcaggctgga atggtgggcg
     3601 taccgagccg aaacgcggtg cggtagcccc gcgatggccg gtacggccat gatggtgcgg
     3661 acggtggacc cggttcggat gatgtgccgg tcaaccatgg ttctccgggg ttggtgatcc
     3721 cgaggtgggt cagcccgagg ttgaaacccc gggctgagtg cacgaagccc ctgttcaggg
     3781 gctggggtga tgggcgcacg gggagctttc tttcgacggt ttgaaaattg agggttctgg
     3841 ctggttatgt tcaggaaaca caacgggcga atcgcaatgc gcccgtccaa cgagatcgaa
     3901 ttgtgcttga cggcatcagg ccccgaatct gtgctgagct ttggatgagg atgctttggc
     3961 actcagtcag taagagcagg gaggttacct gggatcaccg atcctgtcat cggcggtttt
     4021 ctcgacgcgt gaccaccgcg gagctgattt tgcgctgccc aggcccgccg tcaactcaac
     4081 ggtgccgctt gggaggcact atagatcaaa tgttcgcttt tcgtcaactg gtcgttcggc
     4141 tagtcaaggc gacgacatac cctctccgga tctccctggc agggagagcg cagcctctcc
     4201 actccgaacg aaaaagcgct gcgtggtaac acgctacaga cggaagaccg gccacagggg
     4261 gccggtgcta cggacgcaag ggtggggcac ctgcgatacg gacggaaggg tggggcatgg
     4321 taaccgcgat gtggacgaaa agcgtggcgt agtagctggg ctacgatttg gcttctcgac
     4381 tgctcgactc ctcgacttct cgactcgtcg gttcctggac cagctcggtc aggacgccgt
     4441 tgcaggcgcg ggggtgtatg aaggcgatcc agaggccgtg accgccgacg cggggcgcga
     4501 agtcgatcaa ctcgatgccg gccgcagcca gttcgacgag cgtggtggcg aggtcgtcga
     4561 cctggtaggc gacatggtgg aagccttcgc cgcgctttgc catgaagcga ccgatcgcgc
     4621 cctcggagtc ggtcggctgg atcaactcaa cagagccctg cgcgagtgga aagaaggcgg
     4681 cgcgaacgcc ctgcgcggga atatcgaagc gatccgcctc gccgaatccg agcgcctggt
     4741 acgtggcgat cgcggcatcc aggtcagcca cgacgatacc gacgtgatgc agccggccat
     4801 gggtgagcag ctcggaaacc gtcattggcc ggggttcgcc tcaggtgtcg cggccggtgt
     4861 ggagctcttg agcttgccgg cgttcggatc atcaagcggg ctgaccacct tgtcaccgac
     4921 cggctcgccg tcctcgcgca ggatcatgcc gcgcttgcgc cgaccagtca gctcgaagta
     4981 ggcccgggcc gccttgtccg cgatcggcac ggcattggtg gagccttcgc cgccggactc
     5041 gatgaagacg gcgatcgcga tctccggctt gtcgaacggc gcgaacatcg aaaaccaggc
     5101 gtgcgcgtcg tagaccggat cacccgcggc gtcaacgcct ttctggaact cggcagtgcc
     5161 ggtcttgccg ccgagctgga tctcctcctc gccctcggga ttggtatgcg agaacttgcg
     5221 cgtgccgtcg gcgttcataa acgcggtacc gctaaggttg tggacgacgt cgtgcatgcc
     5281 gttcaacacg gtagtcaagt ggtcctggtt gaacttgatc ttgcgcttga tgaccggcgc
     5341 aaactcctgg agcacgttgc cgtcggcatc gaccacactg tggacgatgc gcggccggta
     5401 gacattgccg ccgttgcaga gcgccgccgt gttcatcgcg agctggagcg gcgagaactc
     5461 gatgtagccc tggccgatcg aggcgttgac cgtgtcaccg atcgaccagc cggctccctc
     5521 gtagttgtcg ctcagccact tcacggagcc gacgagtccg gccgtctcga ccgggaggtc
     5581 gatgccggtg atgcggccga accagaagcg cttggtcaga gcggcgtgga ttttgttgat
     5641 gcccagaccg ctgaaactat gctcctcgcc ctgcacctgt gaggcgagac tgaagtcgaa
     5701 gtagtggttg accttgccgt tctcttgccg gcgctgccca gctgatacgc cgacgttgta
     5761 gaagaagacg tcgcacgact gctcgatggc cttctcgacg gccatcgccc cgtggcccgc
     5821 cttcgcccag cagccataga tattgccctt cgattcgtca aagtcatacg gcaccttgat
     5881 gccaccaccg cagaagaact gcgtattggc gtcgatcact ttctcccgca tgccggtcag
     5941 cgccatgaag ggcttgatga ccgagccggg cgggtacttc tgcatgtagg tcttgtcgta
     6001 gaggggacca cgcgggtcgt cgcgcagctc agcgtacttg cgctccgaga tgccctcgac
     6061 gaagagagtg ttgtcgtagg tcgggtagct ggccatggcc agcacctcgc cggtattgac
     6121 gtcgaagatg acgacggcgc cgccctgggt ggtgtgctcc ttgtacggca ggttggcctt
     6181 tgcgtctgcc tcctggcggc gctccatggc gagcggtagc tcctgctgga ggatgcgcga
     6241 gacggcagcc tgcagctcga cgtcaacggt cagctgcagg ttgcggccgg gcaccgccgg
     6301 ctgcgcgccc tcggtgagtc gccgcagctc gcgcccgtta gcgtcccgct cgtagaggta
     6361 gccgcccttg ccgccgcgga gctgctcctc gtagaagtac tccagccctt gcttgccgat
     6421 gatgtcaccc ggcttgtaga agcggtactg cggattgccg tcctcgtcgc gcgaggaaac
     6481 gttgctatcg tcgttcagct cctcgctgtt gaccgggccg gcaaaaccga ggatgtgcga
     6541 catggtcgga ccgcccggat actggcgagt cagcacttcg tcgctgagca cgacgccggg
     6601 cagataaagc tggttcgcgg ccagcttcat cgcgacttcg cgggagatat cggtcttgag
     6661 cagatagggc gtctcgataa acctgtagtt ctggatcaca aagtcgaaga aattgacgat
     6721 ctccacgccc ggcaggtccg cctgataggt gttgaactga gcggcggtgt cggcggagat
     6781 gtcatcgaac ttggcgtagt cgttgaaccg aagctgggcc ttgatccagt tcttcgtgtt
     6841 ctcgaagtcc tgctcggtcg tgtcgccgag gaggcggccg acgcggcggt agacctggtc
     6901 cttggcctcg gccgggatcg agcccgggtt gatcatgacg acgtccggca ggcggagtgc
     6961 ggtcatgagc tgctcgcgaa cgtaggcgag ctgggtcgac tcgagctggc ggatatcggc
     7021 cgggatgacg ctgaccgacc acgacttgcg gttcttggcc atcaacctgc ccttgcggtc
     7081 gacgataacg ccgcgggacg gcttcagctc gcgccagtca gtgatgttgg tggtcgcggc
     7141 ggcctggtaa tccttgccct tgccgatctg catgtagccg aggcgggtga gcaggatgct
     7201 gaagccgccg atgacggctg acttcatcaa caaagcacgg cgggtcagaa agagctggtc
     7261 gacgcggcgg ttggcctgct t
//
LOCUS       DATAOZ010000376         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8836980, whole genome shotgun sequence.
ACCESSION   DATAOZ010000376 DATAOZ010000000
VERSION     DATAOZ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8836980"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="VHR64_09550"
     CDS             <1..1757
                     /locus_tag="VHR64_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3303114.1"
                     /translation="IGFLGGLTGSLFREFAFTLAGSVIVSGVIALTLSPMMCSILLKN
                     TEEGRFAKLVNKVFGALTRWYGRRLDRSLDYKAITGLFAVTILGLVGFLYMHTSKELA
                     PEEDQGIVFAVTKAPKYANIDYVDFYGEKLDKEFQKFPETDLRFVLNGINGPQGGIAG
                     MLLKSWDERKRSSIQLKPLVQAELSKIEGVQAFAFNLPPLPGGPGGLPVQMVINSTAG
                     FQTVYEQMEKLKDAARKSGRFIVSDSDLAYNQPNVEVTIDRTKAQDLGVNMQNLGSTL
                     AVLLGGNYINRFNLEGRSYQVIPQVPRSSRLSPESLGGYYVTTNTGQQLPLSTVVSVR
                     TKTDPNSLTHYNQLNSATFSAVPMPGVTVGSAVDFLEGEAKKLPAGFSHDYLADSRQY
                     VQEGNQLAITFGFALIIIFLVLAAQFESLRDPFVIMISVPMAIVGALIPLFFGMATMN
                     IYTQVGLLTLVGLITKHGILMVEFANELQVNERLDRRSAIEMSARIRLRPILMTTAAM
                     VTGLIPLLTASGAGAASRFSIGLVVVAGMSIGTLFTLFVLPAVYVVLATDHRAAADSE
                     RNKEVNDLDLNSKALRPT"
     gene            1908..>2116
                     /locus_tag="VHR64_09555"
     CDS             1908..>2116
                     /locus_tag="VHR64_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303115.1"
                     /translation="MSISLHPDTPQARTTPSAAAAGVVPGEAGGRGIRTRLFTKYVAL
                     FVAVVAIALLANGLFEVFFYYREHKA"
BASE COUNT          360 a          677 c          667 g          412 t
ORIGIN      
        1 cgatcggctt cctcggcggc ctcaccggtt cgctgttccg cgaattcgcc ttcacgctcg
       61 cgggctcggt gatcgtgtca ggtgtgatcg cgctgacgct gtcgccgatg atgtgctcta
      121 ttctgctcaa gaataccgaa gagggccgct tcgccaagct ggtcaacaag gtgttcggcg
      181 ctttgacgcg ctggtatggg cgcaggctcg accgctcgct cgattacaag gcgatcaccg
      241 gtctgttcgc ggtgacgatc ctcgggctgg tcggcttcct ctacatgcac acctcgaagg
      301 agctggcgcc tgaggaggac cagggcatcg tgttcgcggt gaccaaggcg ccgaaatacg
      361 ccaacatcga ctatgtcgat ttctacggcg agaagctcga caaggaattc cagaagttcc
      421 ccgaaaccga tctgcgcttc gtgctgaacg gcatcaacgg cccgcagggc ggcatcgccg
      481 gcatgctgct gaagtcgtgg gacgagcgca agcgctcgtc gatccagttg aagccgctgg
      541 tgcaggccga gctttccaag atcgagggcg tgcaggcttt cgctttcaac ctgccgccgt
      601 tgccgggtgg ccccggcggt ctgccggtgc agatggtgat caattccacc gcgggcttcc
      661 agacggtcta tgagcagatg gagaagctga aggacgccgc gcgcaagagc ggcaggttca
      721 tcgtctccga cagcgacctc gcctacaacc agccgaacgt ggaggtcacg atcgatcgca
      781 ccaaggcgca ggatctcggc gttaacatgc agaacctcgg ctctacgctc gcggtgctgc
      841 tcggtggcaa ctacatcaac cgcttcaatc tggagggccg gtcctaccag gtgatcccac
      901 aggtgccgcg cagcagccgg ctctcgccgg aatcgctcgg cggctattat gtgacgacca
      961 acacgggcca gcagctcccg ctgtcgaccg tggtctcggt caggaccaag actgatccga
     1021 attcgctgac gcattacaat cagctcaatt cggcgacgtt ctcggctgtg ccgatgcccg
     1081 gcgtgacggt cggatcggcg gtcgacttcc tcgaaggcga ggccaagaag ctgccggccg
     1141 gcttcagcca cgattatctc gcggactcca ggcaatacgt gcaggagggc aatcagcttg
     1201 cgatcacctt cggttttgcg ttgatcatca tcttcctggt gctggcggcg cagttcgaga
     1261 gcctgcgtga cccgttcgtg atcatgatct cggtgccgat ggcgatcgtg ggtgcgctga
     1321 tcccgctgtt cttcggcatg gcgaccatga acatctacac ccaggtcggt ctgctgaccc
     1381 tggtcggcct gatcaccaag cacggcatcc tgatggtgga gttcgccaac gagctccagg
     1441 tcaacgagcg gctcgaccgc cgctcggcca tcgagatgtc ggcccgtatt cgtctgcggc
     1501 cgatcctgat gaccacggcc gcgatggtga ccggcctgat cccgctcctg actgcgtccg
     1561 gcgcgggcgc ggccagccgc ttctcgatcg gtctcgtggt ggtcgccggc atgtcgatcg
     1621 gcacgctgtt cacgctgttc gtgctgcccg cggtctacgt cgtgctggca accgaccacc
     1681 gcgcggcggc cgattccgag cggaacaagg aggtcaacga tctcgacctc aattccaagg
     1741 cgctgcgacc gacctgagct gcaggtcagc gaaccagaag ggcggcagcg gcatccgttg
     1801 ccgccctttt catttgcccc gcagcctgcg gacgccgtta ggcctagggg gagtcctttc
     1861 cgcgttggcg agagacaagt tccggccttc ttgctagatt cgccgggatg agcatctccc
     1921 tccaccccga caccccgcaa gccaggacaa cgccgagcgc ggccgcggct ggcgtcgtgc
     1981 ccggcgaggc gggggggcgc gggatcagga cccggctttt caccaaatat gtcgcgctgt
     2041 tcgtggccgt cgtcgccatc gcgctgctgg ccaacgggct gttcgaggtc ttcttctatt
     2101 atcgcgagca caaggc
//
LOCUS       DATAOZ010000377        10858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8572670, whole genome shotgun sequence.
ACCESSION   DATAOZ010000377 DATAOZ010000000
VERSION     DATAOZ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8572670"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VHR64_09560"
     CDS             <1..255
                     /locus_tag="VHR64_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX3303116.1"
                     /translation="LAIASSLKERPVDLRTVAIGEIGLSGELRAISQLERRVSEALRL
                     GYRRAIVPATLGRRSGDLPSGIEIVRAQTVREAISAGLSD"
     gene            complement(245..1393)
                     /gene="murG"
                     /locus_tag="VHR64_09565"
     CDS             complement(245..1393)
                     /gene="murG"
                     /locus_tag="VHR64_09565"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX3303117.1"
                     /translation="MAQETPLRLIIAAGGTGGHVLPAVAVIRELRSRGITLDAKWIGG
                     NEGIESDVAGDESIPFATIPAGKLRRYLDLKTIRDAVNIPRGVFAARRIVREFKPDVV
                     FSTGGFVSVPTVFAARRRAPILTHEQTTILGLANKINLRSADVLALSFDDTTARVGST
                     KAKVVVTGNPVRSELSAGSAERGRVRFSFSPELPIVFVTGGARGASPLNQRVKAILPQ
                     LLDCAQVIHQTGPASANNDFAELTALKESLPAQVATRYVLTEFVRDEIADVYAMADLV
                     VSRAGAGTIAELSALGKPAIMIPLPLSGGGEQIVNARALADHGAGVMMMQDDATPERL
                     LQEIEDLLRSPDKRATLATAAASLGRPDAAARLADELLALARRPTPSR"
     gene            1501..1929
                     /gene="lspA"
                     /locus_tag="VHR64_09570"
     CDS             1501..1929
                     /gene="lspA"
                     /locus_tag="VHR64_09570"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX3303118.1"
                     /translation="MAVILVDLATKAIVTRALDWGEYRWLIDGWVGLELTRNRGIAFG
                     LGNGSGVISLLVFAGFLMLVWFILRSHLADHPIGAVAFGLAVGGAIGNLVDRISDGAV
                     TDFFVLGPWPRFNVADSALTFGLIALAFIEFRMQGGNQGV"
     gene            1922..2920
                     /locus_tag="VHR64_09575"
     CDS             1922..2920
                     /locus_tag="VHR64_09575"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX3303119.1"
                     /translation="MSEEFLTDDVEWFDIETDAETAAAGEMLTLHPEREEKNTRLDKF
                     VASHAPGMSRSWLQRLIDDGNVLVDGQVRSRTFKVTPGQIIEVSIPPVEEDELIAEAI
                     PLDVVYEDEHIIAINKPAGMVVHPAPGHRTGTLVNALLHYAPDVSMAGSQRPGIVHRL
                     DRDTSGLIVVGRTDAGRLALLEQWAERSVVKEYEAIVRGVPTDPEFAVDAPIGRDPTQ
                     RKRMAVIQSGKDAQTFVTVREAAQIASFADVRIETGRTHQIRVHLAYAGFPIVGDRVY
                     NRFRGAAGGDSQVADRHMLHAGRLVFRNVAGEVVDLRAPLPDDMQAAWRELSEATP"
     gene            2917..4215
                     /locus_tag="VHR64_09580"
     CDS             2917..4215
                     /locus_tag="VHR64_09580"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX3303120.1"
                     /translation="MTDSRSAIIAAFQAALDAVQPDAAVQHVIRVSASGELEVSGRSL
                     GPASHVETIGIGKAAVAMMAGAVNALGDFLTRGYVITKDGHIDRELPARIEVHEAAHP
                     VLDERSVSATHRLLEWVVEIPRDAIVLCLISGGGSALLEEPVEGVSLADFQELTRLLL
                     RAGADIYQLNAVRSQVSRVKGGGLREAIPCDRVVSLLLSDVLGNDPTVIASGPTIPAK
                     ASRGEARDVLTNLQLQQKMPPSINRILAAESLKRSNAHPNDIVEVIADNAKALDAAGQ
                     HLTKASYRVEQDVAPRTGEARDDAREWVMRLRDVPQDIDVVIAGGELTVTVTGNGTGG
                     RNTEFALAAALELERLGVTSWTVASLATDGQDGPTDVAGAIVDHTHASRMRAMHIDPI
                     AALRDNDSCPPLEQTGAVVTTGPTGTNVNDLYFAVRNRPT"
     gene            4272..5192
                     /locus_tag="VHR64_09585"
     CDS             4272..5192
                     /locus_tag="VHR64_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX3303121.1"
                     /translation="MADATPLILDVDTGVDDALAIGLAVSRPDVDLLAVSTLAGNIDV
                     INATENSRRVLGLIGASAIPVHQGASRPLSRVHRNAAHYHGMNGLGDAQLPEVDAPLG
                     PDRGPAAIIRLARQRPGEIVLVATGPLTNIAIALNVFPELPSLLKRFVVMGGSYRNPG
                     NITPHAEFNIWADPEAAQQVFSTDFPEAIAVGLDVSHQTLLLKASWEESGGKSDALAR
                     LLTAVCRRSFVDRAQADFPLHDPLATAVALDPALVATERGVIDVVTDGERDGKTTFTA
                     DPSGSWQVALGVDSGRFVSEFLASYQLSAQ"
     gene            5329..5925
                     /locus_tag="VHR64_09590"
     CDS             5329..5925
                     /locus_tag="VHR64_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303122.1"
                     /translation="MIDAAAFIEQAPVQDQDLLLRLNLYFDVLDQRLRHGQGWFIFNA
                     AGSRLNRIAGFIQAKLASDHASVDSYLMPWRDFALSAYVNEVGLAEIGPAKDGSFSSE
                     AQKREFELARHVTEDTCARLMYSDMLVLIGIRPSAWHEASFLDRMIDERYRQRLATIL
                     LTADMPHTLESEFDEVDPSGMLWSRLFDRMYETSLVAI"
     gene            complement(5929..6885)
                     /locus_tag="VHR64_09595"
     CDS             complement(5929..6885)
                     /locus_tag="VHR64_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX3303123.1"
                     /translation="MGADLDRVSRRVIEVTELSYPVASSIIREIVLAGGKRLRPLLVL
                     LAGRAYDYNDRLDELVTAAAGVELLHIASLVHDDTIDRAALRRGKPTLNSVMSTGATI
                     LIGDFLFAQSAMLAARTGDVRVVSVFASTLGQLCDGQLLELFDSHKTDQEIETYTRRI
                     YGKTASLFAGAAEMGAVLGRAPETHVQHLRQFATEIGLAFQIQDDILDLTRESDELGK
                     PAGNDLRQGTVTLPTLLFAQRISPGSAEQRLLDRVITGDDVADDDIAELIERIRTSGA
                     VDDARAEAQRYQTQALERLAVVPHADTRDLLGNWAEQALASS"
     gene            7181..7654
                     /locus_tag="VHR64_09600"
     CDS             7181..7654
                     /locus_tag="VHR64_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX3303124.1"
                     /translation="MIIVRRSVRRSPVNVQREMERVFRSWVPQAPTFPVATGRAWRPA
                     LEVLETETDLLVTAELAGIDEGSLDVTVDGEVLSIVGTRSGHREEAVCSYWEAGINYG
                     AFQAEVFIPGALRIDDAEATYINGMLRIRLPRATPVPATPRRIPLSSGADKKDES"
     gene            7654..10203
                     /gene="lon"
                     /locus_tag="VHR64_09605"
     CDS             7654..10203
                     /gene="lon"
                     /locus_tag="VHR64_09605"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX3303125.1"
                     /translation="MTDKEKNTAVPEAPDSSPQDETIPMGETVETGPLVDSAPGDSQA
                     EESGESNIVTIPILPLRGTVVFPLTVVPLAAAQPRSLRLIDEVMSGDRGVGLILQNDA
                     EMEGAGPNDVKEIGTLGSILQMMRVPDGSVRLAVQGSERMRIIEWVTEEPFLVAKVEK
                     IPETIEDSVEVEALTRNTLELFQRLVSLVSHLPDELVTAALNVEDPKHLVYLVATNLR
                     MEAEERQALLEEDSVKEKLTRLNAFIQKELDVLELGKKLQTEVQEEMGKSQREYYLRE
                     QLKAIQKELGESSEGEAEIADLRAKLDEAKMPEEADKEARRELDRLSKLPPAAAEYGV
                     IKTYLDWLTSLPWNTSTEGEIDIQKARQILDEDHYGLEKIKDRILEYLAVKKLKQAKV
                     EEAEGPIQNREPILCFVGPPGVGKTSLAQSIARALGREMTRMSLGGVRDEAEIRGHRR
                     TYVGAMPGRIVQAIRRAGTNDPVFVLDEVDKLGSDWRGDPSSALLEVLDPEQNNSFRD
                     HYLDVAFDLSKVMFIATANMLDTIPAPLRDRMEIIELAGYTDEDKLQIARRYLVPKQT
                     DATGLDSVELSWDDDAIMQIIQHYTREAGVRNLERELGAVARKVATRVAEGKDVPTGI
                     GKEQIREYLGRPRFFYEELAARTSQPGVAIGVGVNAVGGDIMFIEATRMPGKGSWTIT
                     GQLGDVMRESAQAALSFVRARARDLGIDPEFLQTNDLHIHVPAGAVPKDGPSAGVAMT
                     IALVSLLTGIPVREDVAMTGEITLRGQVLPVGGIKEKSLAARRAGIKTFVMPRRNEVD
                     LDDLPKGLADQLTFVLADKIEDVLAVAMPEEFHNLRGSNPTILDREAVAAS"
     gene            complement(10269..>10858)
                     /locus_tag="VHR64_09610"
     CDS             complement(10269..>10858)
                     /locus_tag="VHR64_09610"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX3303126.1"
                     /translation="LDLIDGLILTGGADIDPARFGDDEVHPKTYDILPDRDEAEIELT
                     RGALARDLPILGICRGIQVLNVAMGGTLYQDVPDQFSAEIEHRQHEEAIPADEAGHTV
                     TVEPGSLLERTYGEGSIPVNSFHHQAVKGVAPGLVATGASEDGLIEAVESPDCAFVLG
                     VQWHPELMFARHERHVAPFAALVEAAAEAKATARI"
BASE COUNT         1983 a         3298 c         3609 g         1968 t
ORIGIN      
        1 ctggcgatcg cgtcgagcct caaggagcgg ccggtcgacc tgcgcaccgt ggcgatcggc
       61 gaaatcgggt tgagcggcga gctccgggcg atcagccagc tcgagcgccg cgtcagcgag
      121 gcgctgcgat tgggttatcg acgggcgatc gtgccggcga cgctcggccg tcgatccggt
      181 gacctgccgt ccggaattga gatcgtgcga gcccagacgg tccgcgaggc gatcagcgca
      241 ggtctcagcg actaggggtc ggtcgtcttg ccagcgcgag cagctcatcg gccagccgtg
      301 ccgcggcatc gggtcgccca agggacgcgg cggcggttgc cagcgtcgcg cgcttgtccg
      361 ggctccggag caggtcttcg atctcctgca agaggcgctc cggtgtggcg tcgtcttgca
      421 tcatcatgac tccggcgcca tgatccgcca acgcgcgggc gttgacgatc tgctcgccgc
      481 cgccgctcag gggcagcggg atcatgattg ccggcttacc cagggcactc agctcggcga
      541 tggtcccggc cccggcgcgc gaaacaacca ggtcggccat cgcgtaaacg tcggcaatct
      601 cgtcacgcac gaattcggtc agcacgtatc gggtggccac ctgggccggg agggactcct
      661 tgagcgcggt cagctcggcg aaatcgttgt tggccgaggc tggcccggtc tggtggataa
      721 cttgggcgca atcgaggagt tgcggcagga ttgccttgac ccgctgattg agcggagaag
      781 caccacgcgc gccaccggtc acgaagacaa tcggcaattc tgggctaaac gagaaccgca
      841 cgcggccgcg ctcggcactt ccggcgctca actcggagcg gaccgggtta cccgtgacga
      901 cgactttcgc cttcgtcgag ccgactcgtg ccgtcgtgtc gtcgaagctc agcgcgagga
      961 cgtcggccga tctcaggttg atcttgttcg cgagcccgag gatagtcgtt tgctcgtgcg
     1021 tcaggatcgg agcccggcgg cgagcggcga agaccgtcgg cacgctcacg aaacctccgg
     1081 tactgaagac gacatccggc ttgaactcgc gcacaatccg acgggccgcg aatacgccgc
     1141 gcggaatgtt gactgcgtcg cgaatcgtct tcagatcgag gtagcgccga agcttgccgg
     1201 ccgggatggt cgcgaacggg atgctctcgt cacctgcgac atcggactcg atcccttcgt
     1261 tcccgccgat ccacttcgca tcgagcgtga ttccacgcga gcgcagctcc cgaataaccg
     1321 cgacggccgg caggacgtga ccgcccgtac ccccggcggc gataatgagt cgaagaggag
     1381 tttcttgtgc catcccagcc tgctttgcga accgatagaa cccgaccggc aagtatagtg
     1441 gccgccccgg acgaatcccg tgcgcggaat cgtcgcggtg ccgtatccgg gttcgtagca
     1501 ttggccgtca ttctggtcga tctcgcaacc aaggcgatcg tcacgagggc gttggattgg
     1561 ggcgaatatc gttggctcat cgatggatgg gttggcctgg aattgacccg gaatcgcggc
     1621 attgcgttcg gccttggcaa cggatcgggg gtgatctcat tgctcgtgtt tgccggtttc
     1681 ctgatgctcg tctggttcat cctgcgatcg catttggctg atcacccgat tggagcggtg
     1741 gcctttggcc tggccgtggg cggggccatc ggaaacctgg tcgaccggat atcggatggc
     1801 gccgtgaccg atttcttcgt gctcggtcca tggccccggt tcaacgtcgc ggattcggcg
     1861 ctgacgtttg ggctgattgc cctcgccttc attgagtttc gcatgcaagg tggcaaccag
     1921 ggtgtctgaa gagttcctga ccgacgacgt cgaatggttc gacatcgaga ctgacgcgga
     1981 aacggccgcg gccggcgaga tgctgacgct ccacccggag cgcgaggaga agaacacgcg
     2041 gctggacaag tttgttgcaa gtcacgcgcc ggggatgagc cgctcgtggc tccagcggct
     2101 gatcgacgac ggcaacgtcc tggtcgatgg ccaggttcgc agtcggacgt tcaaggtgac
     2161 gcccgggcag atcatcgaag tctcgatccc gccggtcgaa gaggatgagc tgatcgcgga
     2221 ggcgattccg ctcgatgtcg tctacgagga cgagcacatc atcgcgatca acaagccggc
     2281 cggcatggtc gttcatcccg cgcccggtca ccgaaccggc acgctcgtca acgccttgct
     2341 gcactacgcg ccggatgtca gcatggccgg ctcgcagcgg ccaggtatcg tgcaccgact
     2401 ggatcgcgat acctccggac tgatcgtggt cggtcgcacc gacgccggcc gactcgccct
     2461 gctggagcag tgggccgagc gctccgtcgt caaggaatac gaggcaattg tccgcggagt
     2521 tccgaccgac cccgagttcg cggttgacgc gccgatcggc cgcgacccga cgcaacggaa
     2581 gcggatggcg gtcatccagt ccgggaagga cgcgcagacg ttcgtgaccg tccgcgaggc
     2641 ggcacagatc gcgagcttcg ccgatgtccg gatcgagacc ggccgcacgc atcagatccg
     2701 ggttcacctg gcgtacgccg gtttcccgat cgtcggcgac cgcgtctaca accggttccg
     2761 tggcgccgcc ggcggtgaca gccaggtcgc agatcgtcac atgctgcacg ccggacggct
     2821 cgtttttcgt aacgtcgctg gagaggtcgt cgatctgcga gctcccctgc cggatgacat
     2881 gcaggctgcg tggcgcgagc tcagcgaggc tacgccgtga ccgattccag atcggcgatc
     2941 atcgccgcgt ttcaggccgc cctggacgcg gtccagcccg atgccgcggt gcagcatgtg
     3001 attcgagtca gcgcatcggg cgagctcgaa gtcagcggtc gctcgctcgg ccctgcgtcg
     3061 cacgtcgaga ctatcgggat cggcaaggct gcggtcgcaa tgatggcggg cgcggtgaat
     3121 gcgctgggag acttcctgac ccgcggctat gtcatcacca aggacggcca tatcgatcgc
     3181 gaactgccgg cacgcatcga agttcatgaa gccgcgcacc cggtcctgga cgagcgatca
     3241 gtctccgcca cgcatcgcct gctggagtgg gtggtagaga tcccgcgtga tgcaatcgtg
     3301 ctttgcctga tatcgggcgg tggctcggcc ttgctggagg agccagtcga gggcgtttcg
     3361 ctggccgatt tccaggagtt gacgcgactc ctgcttcgag ccggcgccga catctaccag
     3421 ctcaatgccg ttcgatcaca agtcagcagg gtcaagggtg gtgggttgcg agaggcgatc
     3481 ccgtgcgatc gtgtggtttc acttttgctc tccgatgttc tcggtaacga tccgacagtg
     3541 atcgcaagcg gcccaacgat accggccaag gctagccgag gggaggccag agacgttctg
     3601 accaatctcc agctccagca gaagatgcca ccgagcatca atcgaattct tgcagccgag
     3661 agcctcaaac ggtcgaatgc tcatccgaac gatattgttg aggtcattgc cgataacgcg
     3721 aaggcattgg acgccgctgg acagcacctt accaaggcga gctatcgtgt cgagcaagat
     3781 gtcgctccac gtacaggcga ggcgcgtgat gatgcccgcg agtgggtcat gcgactcaga
     3841 gatgtgccgc aggacattga tgttgtcatc gccggcggcg agctgacggt taccgttacc
     3901 ggtaacggga ctgggggtcg caacaccgag ttcgcattgg cagcggcgct ggagctcgag
     3961 cgcctgggcg ttacgagctg gacagttgcg agcctggcta ctgacggaca ggacggtccc
     4021 acggacgttg ccggagcgat cgtcgatcat acccacgcgt cccggatgcg cgcgatgcac
     4081 atcgatccga ttgctgcgct gcgcgacaat gactcctgcc cgcccctcga gcagaccggc
     4141 gcggtcgtga caacgggtcc gaccggcacc aacgtcaacg atctctactt cgcggtccga
     4201 aatcgtccga cctgagccga tatactctcc cgaactcaac gaatcggccg ctcaagtcag
     4261 aaagcgatca cgtggcagac gcgactccgc tcatcctcga tgttgatacc ggtgtggatg
     4321 atgcccttgc gatcggactg gcagtcagcc ggccggatgt cgacctgctg gcggtctcga
     4381 ccctggccgg caatatcgac gtcatcaacg cgaccgagaa ttcgcggcgg gtcctcgggc
     4441 tgatcggcgc ctcggcgatc cccgtgcatc aaggcgcgtc ccgaccgttg tcccgcgtgc
     4501 atcgaaacgc ggcgcattat cacgggatga atggcctcgg cgatgcccaa ctcccggagg
     4561 tcgacgcacc gcttgggccg gaccgcggac cggcggcgat cattcggttg gctcgacagc
     4621 gtccgggcga gatcgtgctg gttgcgaccg ggcccctgac gaacatcgcg atcgccctca
     4681 atgtcttccc ggagctgccg tcgctgctca agcgattcgt ggtgatgggc ggctcatatc
     4741 gcaatcccgg caacatcacg ccgcatgccg agttcaacat ctgggcagac cccgaggcgg
     4801 cgcaacaggt cttttcgacc gatttcccgg aggcgatcgc ggtcgggctc gacgtatccc
     4861 atcagacatt gctcctgaag gcgtcatggg aggagtccgg cggcaaatcg gacgcgctcg
     4921 cccgactgtt gacggctgtg tgccggcgat cgttcgtgga ccgcgcccag gcagatttcc
     4981 ccttgcatga cccccttgcc accgccgtcg cgctcgatcc ggcgctggtc gcgaccgagc
     5041 ggggcgtgat cgatgtggtc acggatggcg agcgtgatgg caagaccacc ttcaccgcgg
     5101 atccgtctgg gtcgtggcag gttgcactag gtgtagattc gggtcgattt gtgtccgaat
     5161 ttcttgcgtc ctaccagctt tctgcgcaat gatgtgcgta cctgctggaa tcaatactcg
     5221 cctttcgggg gatggtcgct cgatagccgg cagggtatca ggaaccatcg ggcatttcgc
     5281 gcgatgccgg atggagtgac ataagaggga acgcaggagg tcgcacccat gattgatgcg
     5341 gctgctttca tcgagcaggc tccggtccag gatcaggatc tgctcttgcg cctaaatctc
     5401 tacttcgatg tgctggacca gcgtctgcgc catggccagg gctggttcat cttcaacgcc
     5461 gccggaagca ggctcaaccg gatcgccggc ttcattcagg ccaagctcgc gtcagaccac
     5521 gcgagcgtcg attcgtacct catgccctgg cgggatttcg ccctgagtgc gtacgtcaac
     5581 gaagtcgggc ttgctgagat cggacccgcc aaggatgggt ccttctcgtc cgaagcgcag
     5641 aagcgcgaat tcgagctcgc ccgccacgtt acggaggaca cctgcgcccg gctcatgtac
     5701 agcgacatgc tcgtgctaat cgggattcga ccgtccgcgt ggcatgaggc ctcgttcctc
     5761 gatcgcatga tcgacgagcg atatcggcag cggctggcga cgatcctgct gaccgccgac
     5821 atgccacaca cgctcgagtc ggagttcgac gaggtagatc cgagcggaat gctctggtcg
     5881 cggctcttcg accggatgta cgagacgagc ctggttgcga tctaggcgtc aggacgacgc
     5941 gagcgcctgt tccgcccagt ttcccaacag gtcgcgcgtg tcggcgtgcg gcacgacggc
     6001 caatcgctcc aaggcctggg tctggtagcg ctgtgcttcc gcgcgggcat cgtcaactgc
     6061 gcccgatgtt cggatccgct cgatcagttc ggcaatgtcg tcgtccgcaa catcatctcc
     6121 ggtgataact cgatcgagaa gtcgctgctc ggctgagccc ggcgagatgc gctgcgcgaa
     6181 gagcagcgtg ggtagcgtga ccgtgccctg gcgcaggtcg ttgccggctg gcttgcccag
     6241 ttcgtccgac tcgcgggtca ggtccaggat atcgtcctga atctggaacg cgagaccgat
     6301 ctcggtggcg aactggcgca aatgttggac gtgggtctcc ggcgcgcgcc cgaggacggc
     6361 gcccatttcg gccgcgccgg caaagagcga ggctgtcttc ccataaatgc ggcgagtgta
     6421 ggtctcgatc tcctggtcag tcttgtggga gtcgaagagc tcgagcagct ggccgtcaca
     6481 aagctggcca agcgtcgagg cgaaaacact aacgacccgc acgtcaccgg tgcgagcggc
     6541 cagcattgcg gactgggcga agaggaagtc gccgattagg atcgtggcac cggtcgacat
     6601 cacggaattg agtgtcggct ttccgcgccg aagggcagcg cgatcgatgg tgtcgtcatg
     6661 gaccagcgac gcaatatgaa gcaactcgac accggcggcg gctgtgacaa gctcgtcgag
     6721 gcggtcgttg tagtcgtagg ctcgaccggc cagcaggacc aggagcgggc gaagtcgctt
     6781 accgccggct aggacgatct cccggattat cgacgacgcg accggatacg acagctcggt
     6841 gacttcgata acgcgccggg aaacgcgatc cagatccgcg cccatgacga gttgaagtcg
     6901 tgctgactct tgaatgtctt cctgtaatcc cgcgccagcc gccattgatc ctgtgcgccc
     6961 tcacccgccc gagcaaaatg ccagcggtca gtatccgggc gatccggtag ccatgtcaaa
     7021 gactggtcaa aacttccaat ctgtaagcga agtcagcgcc gatgataaag attgatacgg
     7081 ggtatagcaa tgttccgatc tgagttgtat aatcatcacg tgcgcttccc ggtcggtacc
     7141 ccagtggttc gatcgggtgt tcgttaggca ggagttggcc atgattattg ttcgacgctc
     7201 ggttcgccgg agtccggtga acgtgcagcg cgagatggag cgcgtctttc ggtcctgggt
     7261 gccccaggcg ccgacgtttc cggtggcgac cggtcgggcc tggcggcccg cgcttgaggt
     7321 tctggagacc gaaacggatt tgctggttac cgctgagctt gccgggatcg acgaaggctc
     7381 gctcgacgtg actgtcgatg gcgaggtgct gagcattgtc ggcacgcgct ccggtcaccg
     7441 cgaagaggct gtttgctcct actgggaggc aggaatcaat tacggcgcgt tccaggccga
     7501 agtcttcatt cccggagctt tgcggatcga cgacgccgaa gcaacctaca tcaacggaat
     7561 gctgcggatc cggttgccca gggcaactcc ggtcccggca acgccgcgtc gaattccatt
     7621 gagcagcggc gctgataaga aggatgagtc ctaatgaccg ataaggaaaa gaacacggca
     7681 gtgcccgagg ccccggatag ctcgccccag gacgagacaa ttccgatggg cgaaactgtc
     7741 gagacaggcc ctctggtcga ctccgcaccg ggcgatagcc aggcggagga gagtggggag
     7801 tcgaacatcg ttacgatccc gatcctgccc ctgcgcggca cggtcgtctt tccgttgact
     7861 gtcgtcccgc tggcggcggc ccaaccccgt tcccttcgcc tgatcgatga ggtgatgtcc
     7921 ggtgatcgcg gcgttggttt gatcctgcag aacgatgcgg agatggaagg tgccggccca
     7981 aacgacgtca aggagatcgg cacgctcggt tcgatcctgc agatgatgcg ggtgccggac
     8041 ggaagtgtcc gcctcgcggt ccagggctcg gagcggatgc gcatcatcga gtgggtcacc
     8101 gaagagccgt tcctggtcgc caaggtcgag aagatcccgg agacaatcga ggattccgtc
     8161 gaggtcgagg cactgacccg gaacacgctg gagctgttcc agcggctggt ttcactggtc
     8221 tcgcacctgc ccgatgagtt ggttaccgcg gcgttgaacg tcgaggatcc gaagcacctg
     8281 gtgtacctgg ttgcgacgaa cttgcggatg gaagcggagg agcggcaggc gctgcttgaa
     8341 gaggacagcg tcaaggagaa gctgactcgc ctgaacgcct tcatccagaa ggagctcgat
     8401 gtcctcgagc tcggcaagaa gcttcagacc gaggtccagg aggagatggg caagtcgcag
     8461 cgggagtact acctgcgcga gcagctcaag gcgatccaga aggagctggg tgagtcgagc
     8521 gagggcgagg ccgagattgc ggacctgcgc gccaagctgg acgaggcgaa gatgccggaa
     8581 gaggccgaca aggaggcgcg gcgcgagctc gatcggctct cgaagctgcc gccggccgca
     8641 gccgagtatg gcgtgatcaa gacctatctc gactggctga cctcgctgcc gtggaacacc
     8701 agcacggaag gcgagatcga catccagaaa gcgcgccaaa tcctggacga ggatcactac
     8761 ggtctcgaga agatcaagga ccgcatcctc gagtacctgg cagtcaagaa gctgaagcag
     8821 gctaaggtcg aggaggcaga gggaccgatc cagaaccgcg aaccaatcct ctgctttgtc
     8881 ggccctccag gtgtcggtaa gaccagcctg gcgcagtcca tcgcccgggc gctcggccgt
     8941 gagatgacgc gcatgtcact tggtggtgtg cgggatgaag ccgagatccg tggtcaccgg
     9001 cggacctatg ttggcgcgat gccgggccgg atcgtccagg ccatccgccg ggcgggaacc
     9061 aatgatccgg tcttcgtact ggacgaggtt gacaagctcg gtagcgactg gcgcggcgac
     9121 ccatcgtcgg ccctgctcga agtgctcgat ccggagcaga acaattcatt ccgggaccac
     9181 tacctggatg tggcgttcga tctctcgaag gtgatgttca tcgcgactgc gaacatgctg
     9241 gacacgattc cggcaccgtt gcgggaccgg atggagatca tcgagctggc cggttacaca
     9301 gacgaggaca agctgcagat cgcgcggcgg tacctggtgc ccaagcagac tgacgcgacg
     9361 gggctggact cggttgagct cagctgggat gacgacgcca tcatgcagat catccagcac
     9421 tacacccgcg aagcgggcgt ccgaaacctg gagcgcgagc ttggcgccgt ggcccgcaag
     9481 gtcgcgaccc gggttgccga gggcaaggac gttccgaccg ggatcggcaa ggagcagatt
     9541 cgcgagtacc tgggtcggcc tcgcttcttc tatgaggagt tggcggcccg gacctcacag
     9601 ccgggtgttg cgatcggtgt cggagtcaat gctgtcggcg gcgacatcat gttcatcgaa
     9661 gcaaccagga tgcccggcaa ggggagctgg acgatcaccg gccagctcgg cgatgtgatg
     9721 cgggagtcgg cccaggcggc gctgtcgttc gtccgggcac gggctcggga cctcgggatc
     9781 gaccccgagt tcctgcagac caacgacctg cacatccacg tcccggccgg cgccgtgccg
     9841 aaggacggac cgtccgccgg tgtggcgatg acgatcgcgc tggtctctct tctgactgga
     9901 atcccggtcc gggaagacgt cgcgatgacc ggcgagatca cgctgcgcgg ccaggtgctg
     9961 ccggttggtg gcatcaagga gaagtcgctg gctgcgcggc gcgccggaat caagaccttc
    10021 gtgatgccac ggcgaaacga ggttgatctg gacgacttgc cgaagggcct ggccgatcaa
    10081 ctgaccttcg tcctggcgga caagattgag gacgtgctcg cggtggcgat gccagaggag
    10141 ttccacaacc ttcggggcag caatccgacc atcctcgatc gcgaagcggt tgccgcgagc
    10201 taagcggaac gcgacgtaaa tgaaacaggc ccggcggaca tccgccgggc ctgtgctcgt
    10261 tgcgatcact agatgcgagc agttgctttc gcctcggcgg cagcttcgac gagcgcggcg
    10321 aagggagcaa cgtgccgctc gtgccgcgcg aacatgagct ccgggtgcca ttgcacgcca
    10381 agcacgaagg cgcaatccgg cgactcgacc gcctcgatca acccatcctc tgaagcgccg
    10441 gtcgctacga gtccgggagc tacacccttg accgcctggt ggtggaacga gttcaccggg
    10501 atcgatcctt cgccgtaggt tcgctcgagg agcgagccgg gctcgacggt gactgtgtgg
    10561 ccggcctcgt cggccggaat cgcttcctca tgctggcggt gctcgatctc cgcgctgaat
    10621 tggtcgggca catcctgata gagcgtgccg cccattgcga cgttcagcac ctggataccc
    10681 cggcagatgc ccaggatcgg gaggtcgcgg gcgagggcgc cacgcgtgag ctcgatctcg
    10741 gcctcgtccc gatcgggcag gatatcgtag gtcttggggt gaacctcgtc atcgccgaag
    10801 cgggccggat caatatctgc gccgccggtg aggatcaggc cgtcgatcag gtctagca
//
LOCUS       DATAOZ010000378         5375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9099319, whole genome shotgun sequence.
ACCESSION   DATAOZ010000378 DATAOZ010000000
VERSION     DATAOZ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9099319"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VHR64_09615"
     CDS             <1..900
                     /locus_tag="VHR64_09615"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEX3303127.1"
                     /translation="PRGVLSARRLVRAFRPDVVFSTGGFVSVPTVIASRGRAPILTHE
                     QTTIIGLANKINLRFADELALSFADTATRVGATKAKTVVTGNPIRSELLSGDADRGRQ
                     QFSFRTNLPLVFVTGGARGASPLNQRIRAILPHLLEQAQVLHQTGPSSANADFADLTA
                     LKKSLPPELATRYVPTEFVREEIADVYAMADLIVCRAGAGTVAELSALGKAAIMIPLP
                     LSGGGEQIVNARALADGGAGVLLMQDDATPERLLREVTSLLTDPERRLKMARSAGELG
                     RRDAAARLADELLALARYPNATR"
     gene            complement(890..2266)
                     /gene="radA"
                     /locus_tag="VHR64_09620"
     CDS             complement(890..2266)
                     /gene="radA"
                     /locus_tag="VHR64_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX3303128.1"
                     /translation="MAKASAKSTFVCQQCGYVSPTMLGRCPNCRTWDSLVETIAPSQS
                     GGSRAASRTTGAAPVRLGDLQTSTIERLPVEIAELNRVLGGGFVPGSLVLLGGDPGIG
                     KSTLVLQAATDIARTGASVLYVSGEESAEQIKLRADRTALGHDTLLVLTETDISAVLE
                     AAEQVRPALLIVDSIQTMSTPELESAPGSVSQVRESAARVMSWAKSTGTPVVLIGHVT
                     KEGAIAGPRVLEHLVDAVLYLEGDRYHQFRILRGVKNRFGSTNEIGVFEMDNAGLVEV
                     RHPSEAFVGDRNANAAGSVVTVTLEGTRPILIELQALVAPSALENPRRVVSGVDLNRI
                     HLLAAVLQKRARLSLGSQDIFVNVVGGLRVDEPAIDLALALAIASSFKERPIDLRTVA
                     IGEIGLSGEIRAISQLERRLSEAQRLGYRRAIVPATLGRRSGDLPSGIEVVRAQTVRE
                     AINAGLSE"
     gene            complement(2398..4848)
                     /locus_tag="VHR64_09625"
     CDS             complement(2398..4848)
                     /locus_tag="VHR64_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX3303129.1"
                     /translation="MAQFDKFTERARKVLQLAQEEAQRFNHNYIGTEHLLLGLVREGD
                     GVAARVLNNMGVQLPKVRSAVEFIIGRGEGAVIGDIGLTPRAKKVLELAVDEGRRLNH
                     HYIGTEHLLLGLVREGEGIAAGVLESLGVNLEKVRAQVMQVVSQSSTQAQTKSQTKTP
                     YMDALGFDLTEAARMNKLGPLIGRENEIERVMQILSRRTKNNPALIGEPGVGKTAIVE
                     GLAQRIVTGDVPETLQNKRLVALDIGALVAGTKYRGEFEERLKKIVAEVKETGSILFV
                     DELHTLVGAGAAEGAVDAANILKPALSRGELQTIGATTLDEYRKYIERDAALERRFQP
                     IQVDEPNIDETYQILTGVRPLYEEHHRLKISDEALKAASTLAARYVTDRFMPDKAIDL
                     IDEASSRVRLRRSAAPPSYKEAMAGLKSIQRELDEAINNQEFELAAELRDREKKLKAR
                     MDQQEQAIRDEAAEEETYVTEEDIAQVVSMWTGIPVYRIAGEESERLLKMESAMHERI
                     IGQEEAITTLSKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTLVAKVLAEFMFGSEDH
                     LIKIDMSEFMERHAVARLVGAPPGYVGYEEGGQLTEAVRRKSYSVILLDEIEKAHPEA
                     FNMLLQIMEDGNLSDAKGRKVDFRNTIIIMTSNVGADQISREMNLGFAMKESSDKVRE
                     REYGQMSDKVMGQLKQMFRPEFLNRIDATIVFHSLSKEQIREIVELELKRVRHQLTEQ
                     GIKLDVSNEAMDLLGERGYDHTYGARPLRRIIQNLIEDPLAEGLLDSRFAENSTIRVE
                     VEDDLLKLVPVEELAAVS"
     gene            complement(5130..>5375)
                     /locus_tag="VHR64_09630"
     CDS             complement(5130..>5375)
                     /locus_tag="VHR64_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303130.1"
                     /translation="GAIADEPAEEPSKLEAAAESVESAKAELAAAEEAGASGAELSEA
                     ADAVDSAATELEAATAAIQESAEGETAAEDSGETAGE"
BASE COUNT          907 a         1799 c         1647 g         1022 t
ORIGIN      
        1 ccgaggggcg tgctgtcggc gcgccgcctg gtccgtgcct tccggccgga tgtagtcttc
       61 agcaccggcg gattcgtgag cgtgccgacg gtcatcgcca gccggggccg cgccccgatc
      121 ctgacccacg agcagacgac gatcatcggc ctcgcaaaca agatcaattt acggttcgcc
      181 gacgaactgg ccctgagctt cgcggacacc gcgacgaggg tcggcgccac gaaagcgaag
      241 acggtcgtga ccggtaatcc gatccgatcg gaactgctgt ccggcgacgc ggaccggggc
      301 cggcagcagt tctcgttccg aaccaaccta cccctggttt tcgtaacagg cggtgcgcgt
      361 ggagcatcgc cgctcaacca acgtatcagg gcaatcctgc cgcatctgct cgagcaggcg
      421 caggttttgc atcagaccgg tccaagctcg gcgaacgccg atttcgccga tctgaccgcg
      481 ctgaagaagt cgctcccgcc tgaattggca acccggtacg tcccaaccga attcgttcgt
      541 gaggagatcg ctgacgtcta cgccatggcc gatttgatcg tctgtcgcgc gggtgcgggc
      601 accgtggccg aactgagcgc gctcggcaag gcggcgataa tgatccccct gccactcagc
      661 ggcggtggag agcaaatcgt caacgcccgg gctctggcag atggtggcgc cggcgtcctg
      721 ctcatgcagg atgacgcgac acccgagcga ctcctcaggg aggtcacatc gcttctgacc
      781 gacccggaac ggcggctgaa gatggcccgt tccgcaggag agctgggccg gagggatgcg
      841 gccgcgcggc tggccgatga gctgctcgca ctggcccgat atccgaacgc tactcgctaa
      901 gtcccgcatt gattgcttcg cgcacggtct gcgcccggac aacctcgatt cccgatggca
      961 agtcgcctga tcgccggccg agggtcgccg ggacgatggc tcgccggtaa ccgaggcgtt
     1021 gtgcctcact caggcgccgc tcaagctggc tgattgcccg gatctccccg ctcagtccaa
     1081 tctcgccaat tgccacggtc cgcagatcga tgggccgctc cttgaagctc gacgcgatcg
     1141 ccagcgcaag cgccagatcg atcgccggct cgtccaccct gagcccaccg acgacgttca
     1201 cgaagatgtc ctggctgccg agcgaaagcc tggcccgctt ctggagcacc gccgcgagca
     1261 ggtgaattcg gttgagatcg acaccgctga ccacccggcg cggattctcg agcgcggatg
     1321 gcgcgaccag cgcctgcagc tcgatcagga taggacgagt gccttcgagc gtgaccgtca
     1381 cgaccgagcc ggccgcattg gcgttacgat cgccgacgaa cgcctccgac ggatgccgaa
     1441 cctcgacaag gccggcattg tccatctcaa agacgccgat ctcattcgtc gagccgaaac
     1501 gatttttgac accccgaagg atccggaact ggtggtagcg atcaccctcg aggtagagga
     1561 cggcatcgac cagatgctcg agcacccgcg gtccagcgat cgcgccctct ttggtgacgt
     1621 gtccgatcag cacgaccggg gttccggtcg acttggccca actcataacc cgtgcggccg
     1681 attcccgcac ctgcgacaca ctacccggcg ccgactcgag ttcgggtgtc gacatggtct
     1741 ggatcgagtc cacgatcagg agcgccggtc gaacctgctc cgccgcctcg agcacggctg
     1801 agatatcggt ctcggtcaat acgagcaggg tgtcgtggcc gagggcggtg cgatcggcac
     1861 ggagcttgat ttgctcggcc gactcctcgc cggagacgta gagaaccgac gcgccggtcc
     1921 gggcaatgtc ggtcgcggcc tggagcacca gcgtcgattt gccgatgccc gggtcgcctc
     1981 cgagcagcac cagtgaaccc ggcacgaagc ctcccccaag gacgcgattc agctcggcga
     2041 tctcgaccgg cagccgctcg atcgtgctcg tctgcaagtc gccaagtcgc acgggcgccg
     2101 ctccggtcgt ccggctcgcg gcccgggatc ctcccgactg ggacggtgca atcgtctcga
     2161 ccagcgaatc ccacgtccgg caattcggac agcggccgag catggtcggg ctgacatacc
     2221 cgcactgctg gcacacgaag gtcgattttg cggaagcctt ggccatgatc acccaatcat
     2281 cgctgaatcg cgtcgctggt cgattttagt acatgcgttc gctcgccgcg aatgccatgg
     2341 acacgaaaac ggggtcccgc aattgggacc ccgttttcgt gattgcatcg atttgcgcta
     2401 gctgaccgcc gcgagctcct cgactgggac gagcttgagc aggtcgtcct cgacctcgac
     2461 gcggatcgtc gagttctcgg cgaaccggct gtcgagcagg ccctcggcca gcggatcctc
     2521 gatcaggttc tggatgatcc gccgcagcgg tcgcgcgccg tacgtgtggt catagccgcg
     2581 ctcgcccagg agatccatcg cctcgttcga gacgtcgagc ttgatcccct gctcagtcaa
     2641 ctggtggcga acccgcttca gctcgagctc gacgatctcg cggatttgct ccttcgagag
     2701 cgagtggaag acgatggtcg cgtcgatccg gttcaggaac tccggccgga acatctgctt
     2761 gagctggccc atgaccttgt cgctcatctg accatactca cgctcgcgaa ccttgtcgct
     2821 cgattccttc attgcgaagc cgaggttcat ctcgcgcgag atctgatcag cgccgacgtt
     2881 cgaggtcatg atgatgatcg tgttgcggaa gtcgaccttg cgacccttgg cgtccgagag
     2941 gtttccgtcc tccatgatct ggaggagcat gttgaaggcc tccgggtgcg ccttttcgat
     3001 ctcgtcgagc aggatcaccg agtacgactt gcgccgcacg gcctcggtca gctgaccgcc
     3061 ttcctcgtag ccgacgtagc ccggaggcgc gccgacgagc cgggcgaccg catgccgctc
     3121 catgaactcg gacatgtcga tcttgatcag gtggtcttcc gagccgaaca tgaactcggc
     3181 cagcaccttc gcgacgagcg tcttgccgac accggtcggg ccgaggaaga tgaagctgcc
     3241 gatcggccgc ttcggatcct tgagtccggc gcgggctcgg cggacggcct tggagagcgt
     3301 ggtgatcgcc tcctcctggc cgatgatccg ctcgtgcatt gccgattcca tcttcagcag
     3361 ccgctcggac tcctcgcccg caatccggta gaccggaata ccggtccaca tcgagacgac
     3421 ctgggcgatg tcctcttcgg tgacgtaggt ctcctcttcg gcggcctcgt cacggatggc
     3481 ctgctcctgc tgatccatcc gggccttgag cttcttctcc cggtcccgca gttcggcagc
     3541 caactcgaac tcctggttgt tgatcgcctc gtcgagctcg cgctggatcg acttcagacc
     3601 cgccatcgcc tccttgtagg agggcggagc ggcggagcgc cgcagtcgca cgcgcgatga
     3661 cgcctcatcg atcaggtcga tcgccttgtc gggcatgaac cgatcagtca cgtaccgggc
     3721 tgccagcgtt gatgcggcct tcagggcctc gtcgctgatc ttgagccggt ggtgctcctc
     3781 gtagagcggg cgcacgccgg tcaggatctg gtatgtctca tcgatattcg gctcgtcgac
     3841 ctggatcggc tggaagcgcc gctcgagcgc cgcatcccgc tcgatgtact tccggtactc
     3901 atcaagcgtg gtcgcgccga tcgtctggag ctcgccgcgg gagagcgccg gcttcaagat
     3961 attggccgcg tcgaccgcgc cctcagccgc acccgcaccg acgagcgtgt ggagctcatc
     4021 gacaaagagg atcgagccgg tctccttgac ctcagccacg atcttcttga gccgctcctc
     4081 gaactcgcca cggtacttgg taccagcgac cagcgctccg atgtcgagcg caaccaaccg
     4141 cttgttctgc aatgtctcgg gaacgtcgcc ggtcacgatc cgctgggcca gaccctcgac
     4201 gatcgccgtc ttgccaacgc ccggctcccc aatcagggca gggttgttct tggtgcggcg
     4261 cgagaggatc tgcatgacgc gctcgatctc gttctcgcga ccgatcagcg gcccgagctt
     4321 gttcatgcgc gccgcctcgg tcaaatcgaa tccgagggca tccatatatg gagtcttggt
     4381 ctgggacttg gtctgcgcct gggtgctgga ctggctgacg acctgcatga cctgcgcgcg
     4441 caccttctcg aggttcacgc ccagcgattc gagcacgccg gcggcgatgc cctcgccctc
     4501 gcggaccagg ccgagcaaga gatgctctgt tccaatgtaa tgatgattga ggcgccggcc
     4561 ttcatcgacc gcgagctcga gcaccttctt ggcgcgtgga gtcaggccga tgtcgccgat
     4621 caccgctccc tcgccgcgac cgatgatgaa ctcaacggcg gagcggacct tggggagctg
     4681 gacgcccatg ttgttgagga cccgtgcggc gaccccatcc ccttcccgca ccaggccgag
     4741 caagaggtgc tccgtcccga tgtagttgtg gttgaatcgc tgggcttctt cctgggcgag
     4801 ctgcaacacc ttgcgtgcgc gttcggtgaa cttatcaaac tgtgccatta ctgcggctct
     4861 cccaatcgca aacgccatcg gatcggttac tggcgaggcg ttcttatctt ctagttaagc
     4921 atacgggatg agggagtacc cggacgtact ctctctgctc tatgaccaca ctacacgcga
     4981 atcgaacaat tccattctac tcccgaatca attacggtct actaccgaat caagtagggc
     5041 aggagcgttg cgctcctgcc ctatctaact gttcaaatta acagtacgtt ccggtcctgc
     5101 gccggtcaac tgcccactct ggcagttgat tactcgccgg ccgtctcccc ggagtcctcg
     5161 gcggcggttt caccctccgc cgactcctgg atcgcggcgg tggccgcctc gagctcggtg
     5221 gctgcggagt cgaccgcgtc agccgcctcg ctcaactcgg cgccggaggc cccagcctcc
     5281 tcggccgcgg cgagctcggc cttggcggat tcgacgctct cagccgccgc ctcgagcttc
     5341 gagggctctt cggcgggctc gtcagcaatc gcgcc
//
LOCUS       DATAOZ010000379        11084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5419182, whole genome shotgun sequence.
ACCESSION   DATAOZ010000379 DATAOZ010000000
VERSION     DATAOZ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5419182"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VHR64_09635"
     CDS             complement(<1..1135)
                     /locus_tag="VHR64_09635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303131.1"
                     /translation="MTAVSQSKQRRLHMPHIPTEWASRPVRASLLGDPVYLNFWLSRL
                     FSQTAQGAILYALLILITDQTDQSIYTSLFVICAIVPALVFGLPAGISVDSLPRVPLM
                     VVLNFMRVLFVMALVDANLSMVGIFAVALGLWTIHQFYAPAESALMASLVDRQLYTQA
                     QSLSNLALSLAQLLGLVVIAPVTLRLAGPPALFTLCGMLWIAAAILVGLLPAARHAGA
                     LNRKRRAGARESLLNGWHLVRTDHAIYEVFIDDMLVGIGGSALVVLMPLYLKGVLNTG
                     AENTVFVFAPAALGLLIGLRAAPILARAIGGRRTATMALMLFSGTVAMFGYVNYVLTF
                     LDDVLNLPVSEIARTLSLAPLTLMVMLLSVPSGMATAIGSVAAR"
     gene            complement(1212..1724)
                     /locus_tag="VHR64_09640"
     CDS             complement(1212..1724)
                     /locus_tag="VHR64_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303132.1"
                     /translation="MSSFDDLKDRLHTSDHQLWLLIGLCVALLVTVEAVQEAVEGAWP
                     HQRRLTRMDLSEQRSQPVWALVALLILPAALLAVFNVAMMVWKDVPRTDEMTTGGALL
                     GIGWVLFLLASVDAFRVRPLISRAGPVFPIALVVVLAAAIALLLSAFLDVRPSVDTLR
                     NAIPLLAESD"
     gene            complement(1749..2921)
                     /locus_tag="VHR64_09645"
     CDS             complement(1749..2921)
                     /locus_tag="VHR64_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX3303133.1"
                     /translation="MARRAEAFGRSPWTFIDQFFDHHPDLIYFGNGAPAAELYPVAAL
                     QKGAAAAWAEIGPTELDYGELQGYPPLRRMIADRMAAQGFDVDPEQILLTFGSQQGID
                     ISARLMLDPGDPLAAEGPTYIGALQAFDAYEPDYHLYPVDQSGLRVDELIADVEQTGR
                     APKLIYVIPNFQNPSGQTMSEERRATLVAFAEEIGALIVEDDPYGEFWYDAPPPPALR
                     IRSDRVAYLGTFSKTIAPGLRSGWIVVPEGMMGRALMAKESSEVCGVRPTTRVVYEAA
                     RDFLDEHVAEGRAFYRQRRDSIMSALTDAMPAGVTWSTPGGGFFVWVTLPEGLTASAA
                     LPVAAEHGVGFLPGRFFYPAADGDDQSLRLSFSSLPEARIQEGIARLGAALDRLLA"
     gene            3237..4505
                     /locus_tag="VHR64_09650"
     CDS             3237..4505
                     /locus_tag="VHR64_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX3303134.1"
                     /translation="MAGGDQQRRGLAGSDHYLDLVDLYAARATRAKTVVAHDAKPPVV
                     HSMGRSERLTVRAARSSAHGFRSRRIRIASAATAVLLIFSVLAGTALADQQYRVREGD
                     TITIVGERFGVDPVAILKASGLDNPEYLYPGQELTIPGIADGDLGTGWVLGTYEVVYG
                     DTIGSIAWNLHVSASDLLEINGLSADAIIYPGQVLVVPDTPATKEETLAQLDELSSEQ
                     VTGESEEAAAETVADQTASAQLPSQAWVSVPTYVQRRNLSCEYASTYIATAAFGPGID
                     ESTFWSSIPVTQNPHYGYRGDIDGWWGNYTDYGIYPEPLVPVLNAYGYGGEVFYGESD
                     PTYLMQQLAWGRPVVVWLAMWGDTGIRFDDKGSYTIFSGEHVMTAYGYDETGVYLSDP
                     ATGGTKYYDWATFLWMWGTSDGMSLAVYPL"
     gene            4792..6675
                     /locus_tag="VHR64_09655"
     CDS             4792..6675
                     /locus_tag="VHR64_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3303135.1"
                     /translation="MLRGGGGGRANRFFPTTDIDPKTLQRPDSETVRRVARFFTPYKS
                     RVAISIVAILITAAIGLVNPILLKLIIDDAIANDDLQKLYLYVSLMIILPIISGLIGV
                     GQTWLNTVIGQRVMSDLREQLYGHLQQMPLRFFTETRTGEIQSRLSNDVGGIQRVVTD
                     TATSIVSNLAVAISTTIAMWLLDWRLALLSLAMLPIFAVLTRKVGQARREVTSSTQRS
                     LADMTTILEETLSVSGVLLTKAFGRQAHEIDKFRAENKNLIRFQIREQMIGRWFFMII
                     GTFFSITPALVYLVAGRDIINGGTMTVGSIVAFTTLQSRLFFPLGQLLNIQVEIHGAF
                     ALFDRIFEYLDLPIALKDEPGAVSLSHDEVQGRVTFRDVSFVYDRPADPEADGAQPFT
                     LTNISFEAEPGQLVALVGPSGAGKSTISMLIPRLYDVDSGTVEIDGRDVRTITLESLG
                     QLIGMVTQETYLFHATVRANIAYGKLNATDAEIEAAARAAAIHDRITDLPERYETVVG
                     ERGYKLSGGEKQRIALARVILKDPRILILDEATSALDTHSERLIQAALERLMAGRTTI
                     AIAHRLSTVLAADQILVIERGRNVERGRHAELLSLDGLYARLYREQFIELEQPALVPA
                     RVR"
     gene            complement(6629..7903)
                     /locus_tag="VHR64_09660"
     CDS             complement(6629..7903)
                     /locus_tag="VHR64_09660"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303136.1"
                     /translation="MAEPGFGPPSGIASRRDKAGIIALLSANAISLTGNQVTMFALPW
                     FVLETTDSAAQTALVAATQMAAFLLAAVAGGVVVDRFGPKWLSVVSDLASGLSIAAIP
                     ILMHTIGLRLWQVLVLAFLGAILDSPGGNARYGIVTDLIDLTGWRPERVYSAFSSADG
                     VARIFGPILAGGLIAWIGAVNALWVDAASFAVSALLITVLVPLTTRPLRPESTFMGDV
                     QAGWEYLRSRALLIRFFSLIVCVNLTAVPLAAVVIPKIAHDHYGSSRIGGVMLAADGI
                     GVILGGLVFGIIGRRLTAYRSMLVALVLLFLSMLLLSGLFPVPISVAALALTGIGFGV
                     MVPNNQTVFRRITDVEFRGRLLGLRDAVVGISSPMMVVIVGLLLDLSPVRLVIMLLAL
                     INLGVVIWYLRESVFAQLNAHEPVQELVVQAR"
     gene            8098..9258
                     /locus_tag="VHR64_09665"
     CDS             8098..9258
                     /locus_tag="VHR64_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303137.1"
                     /translation="MPEFQVGDIVRVEMPRGYSTRGVLGISLLFSTSPEARFEGAIGT
                     VTEINPVGSHSVHQYLVDFRTHDNSRVGIPWQAQWFREEWLALKERPTATEAEAAKTG
                     PEATWPTRPEPAAESAAGGIAHPEAKIFTEGKKDFAPPGMDPSQATGTVPPAAYGVAS
                     PAATGQVAPNESDAEVQQRAADADSVMPWSADVRTQTDLPSSTASEQTDAVIVTSTEW
                     VATESHGDSVTVMHGQETVVTPVTSREPEISADAQTSSPAREAAGAEKSEGFTGPDRA
                     DDRSGADVPAFEPTADTMSGTIESTTPSSAPGSVQGQPSADATIVEQGDGFVRVHGMS
                     ACPEGFPIKGNANSGIYHVPADASYQRTIPEVCFASEDVAIANGYRAPKGRG"
     gene            complement(9504..10796)
                     /locus_tag="VHR64_09670"
     CDS             complement(9504..10796)
                     /locus_tag="VHR64_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEX3303138.1"
                     /translation="MSDTTEKPLGFETLALHAGQDPDPNTLSRAVPLYQTTSYVFKDP
                     EHAARLFGLQEFGNIYTRIMNPTTDAFEQRVAALEGGVAALAFASGQAAETAAILNIA
                     RAGDDIVSTTSLYGGTYSLFQYTLPKMGINVIFCEPTPEAVAAAITPKTKAVYSETVG
                     NPNLITLDIEAVAKVAHDNGIPLIIDNTIPSPYLVNPIKHGADIVIHSATKFLGGHGT
                     SIAGIVVDGGKFDWAASGKFPELTDPDPSYHGMKFWDTFGAITYAIKLRVSVLRDTGA
                     AISPFNSWLILQGIETLPVRMERHSENAVAVARFLTEHPKVSWVNYPGLPTHPAYAQA
                     SKYHYRGKFGAIMGFGVKGGLEAGRKLIESVKLFSHLANIGDAKSLIIHPASTTHSQL
                     TEEEHTLTGVTPDYVRLSVGIETVSDLIDDLDQALAKV"
     gene            complement(10901..>11084)
                     /gene="metX"
                     /locus_tag="VHR64_09675"
     CDS             complement(10901..>11084)
                     /gene="metX"
                     /locus_tag="VHR64_09675"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641775.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX3303139.1"
                     /translation="SDWLFPPDAICQFAARTAAAGKEVRYDELDSPHGHDAFLKEWDQ
                     LTAVLTPFLARFGGGA"
BASE COUNT         2157 a         3609 c         3514 g         1804 t
ORIGIN      
        1 agcgtgccgc cacgctgccg atcgcggtcg ccatccccga cggaacggag agcaacatga
       61 ccatcaacgt caaaggcgcc aggctgagcg tccgcgcaat ttcactcact ggcaggttca
      121 acacgtcatc gaggaaggtc aggacgtagt tgacgtaccc gaacatggcc acggtcccgg
      181 agaagagcat cagcgccatc gttgccgtcc gccgtccccc gattgcgcgc gccaggatcg
      241 gcgccgcgcg gagaccgatc aacaggccca acgctgccgg cgcgaagacg aagaccgtgt
      301 tctccgcgcc cgtgttgagt acgcccttca gatagagcgg catcaatacg accagcgcgc
      361 tcccgccgat accgaccagc atgtcgtcaa tgaagacctc gtagatcgcg tgatccgtcc
      421 gcacgaggtg ccagccgttg agcaggctct cccgcgcgcc ggcgcgccgc ttccgattga
      481 gcgcgccggc gtgccgcgcc gccgggagga gaccgaccag aatcgcggcc gcgatccaga
      541 gcatgccgca gagcgtaaag agcgccggcg gaccagccag tcgcaacgtc accggcgcga
      601 tcaccaccag accgaggagt tgggcgagcg acagggccaa attcgagagc gactgcgcct
      661 gggtgtagag ctggcgatcc accagcgagg ccatgagcgc gctttcagcc ggcgcataga
      721 actggtggat cgtccacaag ccgagcgcga ccgcgaagat accgaccatc gacagattcg
      781 cgtcgaccag cgccatcacg aacagcacgc gcatgaaatt cagcaccacc atcagcggca
      841 cacgtggcag cgagtcgacc gagatcccgg ccggcaaccc gaagaccagt gccggaacga
      901 tcgcgcagat gacgaagagc gaggtataga tcgactggtc agtctggtcc gtgatcagga
      961 tcagcagcgc gtacaggatg gcgccctggg cggtctgcga gaacagccgc gacagccaaa
     1021 aattgaggta taccggatca cccaggagcg aggcgcgcac ggggcggctg gcccactccg
     1081 tcgggatgtg cggcatatgc aatcgacgct gttttgactg agacacagcg gtcatcacgt
     1141 actccgagcc agcaatgctc agaccgagac taccgcatcg aacacggcag ggctatcccc
     1201 gattggaccg atcaatcgct ctccgcaagg agcgggatcg cgttccgcag tgtatcgacc
     1261 gacggccgga catcgagaaa cgcagacaac aaaagcgcaa ttgccgccgc cagcacgacg
     1321 accagagcga tcgggaagac gggcccagcc cgggagatca gcggtcgcac ccggaacgca
     1381 tcgacgctgg ccagcaggaa gagtacccat ccgatcccga gcaaggcgcc gccggtcgtc
     1441 atctcatcgg ttcgggggac gtccttccag accatcatcg cgacgttgaa gacggcgagg
     1501 agcgcggcgg gcaggatcag gagggcgacc aatgcccaga ccggctgcga gcgctgctcg
     1561 ctgaggtcca tccgggtcag gcggcgttga tgcggccagg caccctcgac ggcctcctgg
     1621 accgcctcca cggtcacgag caaggcaacg cacagcccga tcagcagcca gagctggtga
     1681 tcgctcgtgt ggagccgatc cttcagatca tcgaacgaac tcattggcat ccttcggtcg
     1741 gcacgggatc aggcgagcag gcgatcaagg gcggctccca gccgggcgat gccctcctga
     1801 atccgcgctt cgggcaggga tgaaaaactc agccgcaggg actggtcgtc gccatcggca
     1861 gccggataga agaagcggcc gggcagaaag cctacgccat gctcggccgc cacaggtaac
     1921 gcggcgctgg ccgtcagacc ttccggcagt gtcacccaca cgaagaagcc gccgcccggc
     1981 gtgctccagg tgacgccggc cggcatcgcg tcggtcaatg cggacatgat gctgtctcgc
     2041 cgctgccggt agaaggcccg cccctctgcc acgtgctcat ccaggaagtc ccgtgccgcc
     2101 tcgtagacga cacgcgtcgt cggccgcacg ccacagacct cggacgactc cttggccatc
     2161 agcgcgcggc ccatcatccc ctccggcacg actatccaac cggagcggag cccgggcgcg
     2221 atcgtcttgg aaaaggtgcc gaggtaggcc acgcggtcgg accgaatgcg cagggccgga
     2281 ggtggcggcg cgtcgtacca gaactcgccg tacgggtcgt cttcgacgat cagcgcaccg
     2341 atctcctcgg cgaaagccac cagcgttgcg cggcgctctt cagacatggt ctgaccggat
     2401 gggttctgga aattggggat gacgtagatc agcttcggcg cgcgacccgt ttgctcgaca
     2461 tcggcgatca gctcgtcaac gcgcaacccg gactggtcga cgggatagag atggtaatcg
     2521 ggctcgtagg cgtcgaaggc ctgcagagcg ccgatgtagg tcgggccttc ggctgcgagt
     2581 ggatcacccg ggtcgagcat cagccgtgcc gagatatcga tgccctgctg gctgccgaac
     2641 gtgagcagga tttgctcggg atcgacatcg aagccctgcg ccgccattcg gtccgcgatc
     2701 atgcgacgca atggcggata tccctgcagc tcaccgtaat cgagctccgt cggaccgatc
     2761 tcggcccagg cggccgcagc acccttctgc agggccgcga ccggatagag ttcggcagcc
     2821 ggggcgccgt tgccgaagta gatcagatcc gggtggtgat cgaagaactg gtcgatgaag
     2881 gtccatggcg agcgaccgaa cgcttcggcc cgccgcgcca ttcgttccag taactccgca
     2941 cgcacctgat gggccacacc tgtctccgga tccaacaccc gccaaaatcg tcgcaaaagt
     3001 atgacatctc gccatagaac gaatcgacgc cacgcctcgt tccgccctgg tgccgggctg
     3061 gcaaggatct ttctctcgcc gaagactgtg actcaaggaa acaaagcaac ggttcaccca
     3121 atcttgactc atcggcacaa aacctcttgc gtgtacgtac accctgtcgt aggatgggtt
     3181 tgcccgctcg tttccatccc ccgcgaaatc agcttcagtc agcggagggc agggagatgg
     3241 caggtggaga tcagcagaga cgtggactcg cggggagcga ccactatctg gacctggtcg
     3301 acctttacgc cgcccgcgcg acccgcgcaa agactgttgt tgcgcacgac gccaagccgc
     3361 cggtcgtcca ttctatgggc cgatccgagc gattgacagt ccgcgccgct cgatcatcgg
     3421 cgcacggctt tcgctcgcgc cggatccgaa tcgcttcggc agccacggcg gttctcctga
     3481 tcttctcagt cttggcgggg accgccctgg ccgatcagca ataccgcgtc cgcgaaggcg
     3541 acacgatcac cattgtcggc gagcggtttg gcgtcgaccc ggtcgcgatc ctcaaagcga
     3601 gtgggctgga caatcccgag tacctctacc ccggccagga gctgacgatc ccgggcattg
     3661 ccgatggcga cctcggcacc ggctgggtgc tcggcaccta tgaggtggtc tacggcgaca
     3721 cgatcggttc catcgcctgg aacctgcacg tcagcgcctc cgatctgctc gagatcaacg
     3781 ggctgagcgc cgacgccatc atctatcccg gccaggtgct ggtcgtaccc gacactcccg
     3841 cgacgaagga agagacgctg gcgcagctcg atgagctgag cagcgagcag gtcaccggcg
     3901 agagcgagga agcagccgca gaaaccgtgg ccgaccagac cgcgagcgcc cagttgccgt
     3961 cacaggcatg ggtcagcgtc ccgacatatg tccagcgccg caacctgagc tgcgagtatg
     4021 cctcgacgta catcgcgacg gccgccttcg gtccgggcat cgacgagtcc accttctgga
     4081 gcagcatccc ggtcacccag aacccgcact acgggtatcg cggcgatatc gacggctggt
     4141 ggggcaacta caccgactac ggcatctacc cggaaccact ggtgccggtc ctgaatgcgt
     4201 acgggtatgg cggtgaggtc ttttacggcg agtccgaccc cacgtacctg atgcaacagc
     4261 ttgcctgggg gcgaccagtt gtggtctggc tggcgatgtg gggcgatacc ggcattcgct
     4321 tcgacgataa agggagctac accatcttct ccggcgagca cgtcatgacc gcctacggct
     4381 acgacgaaac cggcgtctac ctctccgatc cggccaccgg cggcacgaag tattacgact
     4441 gggcgacctt cctctggatg tggggaacca gtgacggcat gagcctggcc gtctacccgc
     4501 tgtaggacat taccgagccg aaacgtaggg cgcatccgtc tccgagcaac tcgtccccgt
     4561 tcaggcatcg gttgcccgtt gagattgtcg gggcctacgg ccaaagcgcg gcacggcggc
     4621 cgcgcgctac gaaagatcgc gccagcggca cggcgccacg cgctacgaca aattggcgcc
     4681 accaaaatgc atttccctgt ctgctccggt ccctggtagc ctgcccgctt ggacaggctg
     4741 ccggcgcgcg ttccgccacg tatgcttcgg aattgaacaa ggcggacgcg catgctgcga
     4801 ggcggtggcg gtggccgggc aaaccggttc ttccccacga ccgatatcga ccccaaaacc
     4861 ctgcaacgac cggactcgga gaccgtccgg cgagtcgccc gtttcttcac gccgtacaag
     4921 agccgggtcg cgatctcgat cgtcgcgatc ctgattaccg ccgcgatcgg cctggtcaac
     4981 ccgatcctgc tcaagctgat catcgacgac gcgatcgcga acgatgatct gcaaaagctc
     5041 tatctgtacg tcagcctgat gatcatcttg ccgatcatct ccggcctgat aggcgtcggc
     5101 caaacctggc tcaacacggt catcggtcag agggtgatga gcgacctgcg cgagcagctc
     5161 tatggtcacc tccagcagat gccgctccgc ttcttcaccg agacccgcac cggcgagatc
     5221 cagtcccgcc tcagcaacga tgtcggcggc attcagcggg tcgtgacaga tacggcgacg
     5281 agcatcgtca gtaatctggc agtcgcgatc tcgacgacca tcgcgatgtg gctgctcgac
     5341 tggcgattgg ccctgctctc gctcgccatg ctgccgatct tcgcggtcct gacgcgcaag
     5401 gtcggtcagg cacggcgcga ggtcacctcc agcacgcagc gctcgctggc cgacatgacg
     5461 accatcctcg aggagacgct ctcggtcagt ggcgtcctcc tgaccaaggc cttcggccgg
     5521 caggcgcacg agatcgacaa gttccgggcc gagaacaaga acctgatccg gttccagatt
     5581 cgcgagcaaa tgatcggccg ctggttcttc atgatcatcg gcaccttctt ctcgatcaca
     5641 ccggcgctcg tctacctggt cgccgggcgc gacatcatca acggcggcac gatgacggtc
     5701 ggctcgatcg tggccttcac cacgctccag agccgcctat tcttcccgct cgggcaattg
     5761 ctgaacatcc aggtcgagat ccacggcgcc ttcgccctct tcgaccggat ctttgagtac
     5821 ctggatctgc caatcgcgct caaggacgag cccggcgccg tctcactcag ccacgatgaa
     5881 gtccagggac gggtcacctt ccgcgatgtc tcattcgtgt acgatcgccc ggcggatccc
     5941 gaagccgacg gcgcgcagcc attcaccctc acgaacatct cgttcgaagc tgagccgggc
     6001 cagctcgtgg cgctggtcgg acctagcggg gccggcaaat cgacgatctc gatgctgatc
     6061 ccgcgccttt acgacgtcga ctccggcacg gtcgagatcg atggccggga tgttcgcacg
     6121 atcacgctcg agtcgctcgg ccaattgatc ggcatggtta cccaggagac gtatctgttc
     6181 cacgccaccg tccgggccaa catcgcctac ggcaagttga acgcaaccga tgccgaaatc
     6241 gaggcggcgg cgcgcgcggc ggcgatccat gatcggatca cggacctacc cgagcggtac
     6301 gagacggtcg tcggcgagcg gggatacaag ctttcgggcg gagagaagca gcggatcgca
     6361 ctggcccggg tcattctcaa ggatccgcgc atcctgatcc tggacgaggc caccagcgcg
     6421 ctcgataccc attccgagcg gctgatccag gcggcgctcg agcgattgat ggccggccgg
     6481 accacgattg cgatcgccca ccgcctgagc acggtactgg cggcggatca gatcctggtc
     6541 atcgagcggg gacggaacgt cgagcgcggt cgtcacgccg agttgttgtc cctggacggc
     6601 ctgtatgcac ggctctaccg ggagcagttc atcgagcttg aacaaccagc tcttgtaccg
     6661 gctcgtgtgc gttgagctgc gcgaagaccg actcgcgcag gtaccagatc accacgccca
     6721 ggttgatcag cgcaagcagc atgatgacca ggcgcaccgg cgagagatcg agcaacagcc
     6781 cgacaatgac caccatcatc ggcgaagaga taccgacgac ggcatcccgc aggcccagca
     6841 accggccccg gaactcgacg tcagtgatgc gccggaagac ggtttgattg ttcggcacca
     6901 tgacgccgaa accgattccg gtcagggcca gcgccgcgac cgatatcggc accgggaaca
     6961 gcccggagag cagcagcatg ctcaggaaga gcagcaccag tgcgaccagc attgaccggt
     7021 aggcggtcaa gcggcgccca atgatcccga agaccaggcc gccgagaatc acgccgatcc
     7081 cgtccgcggc cagcattacc ccgccgatcc gggacgaacc gtaatggtcg tgcgcgatct
     7141 tggggatgac gacggcggcc agcgggacgg ccgtcaggtt cacgcagacg atcagcgaga
     7201 agaagcggat cagtagggca cgcgaacgca ggtactccca tccggcctgg acatcgccca
     7261 tgaaggtcga ctcgggacgc agcggccggg tggtgagtgg gaccagaacg gtgatcagga
     7321 gcgcgctgac cgcgaatgag gcggcgtcca cccacagcgc attgaccgca ccgatccagg
     7381 cgatcaaccc gcccgccagg atcggaccga agatccgggc cacgccatcc gccgaggaga
     7441 aggcggagta gacgcgttca ggtctccagc cggtcaggtc gatcaggtcg gtgacgatcc
     7501 cgtaccgggc attgccgcct ggcgagtcga ggatcgcgcc caggaaggcc agaaccagca
     7561 cctgccagag ccgcagcccg atcgtatgca taaggatcgg aatcgccgcg atcgagagcc
     7621 cgctggcgag gtcggaaaca acggagagcc acttgggccc gaaccgatcg acgactactc
     7681 cgccggcaac ggcggccagc aaaaacgccg ccatttgggt ggcggcgacc agggccgttt
     7741 gggcggcgct atcggttgtc tcgagcacga accagggcaa tgcgaacatc gtcacctggt
     7801 tgccggtcag ggagattgcg ttcgccgaga gcaaggcgat gatgcctgcc ttgtcgcgtc
     7861 gcgatgcaat ccctgaaggg ggtccgaatc ccggttcagc catgcaacat gaccgaaacg
     7921 gagacgttaa tcaccggtat tccgaaaatc ccccacatgc gacagatagt agcggtaatg
     7981 tcgccggaat gcatcttttt cgggcgagtc ggacggagtc tcgaaggggc ggatatatac
     8041 tctccgcaat cgcaatgcgc accgctgccg tggtggtgac gaatttggga gcgaccgatg
     8101 ccagagtttc aggtaggtga cattgtccgg gtcgagatgc cacgtgggta cagcacccgg
     8161 ggcgtactcg gcatcagcct gctgttcagc acctcgcctg aagcacgctt cgaaggcgcg
     8221 atcggcaccg tgaccgagat caacccggtc ggctcccatt ccgtgcacca gtacctggtc
     8281 gatttccgca cccacgacaa cagccgggtc ggcattccgt ggcaggcaca gtggttccgt
     8341 gaggaatggc tcgcgctcaa ggagcgaccc acggcaaccg aggcggaagc agcaaagaca
     8401 ggcccggagg caacctggcc gacgcgtcct gagccggccg ccgaatccgc tgccggtggt
     8461 attgcccacc cggaggcaaa gatcttcact gaaggcaaga aggactttgc gcctcccggc
     8521 atggacccgt cccaggcaac tggcacggta ccgcccgcgg cgtatggtgt cgcctccccg
     8581 gccgcgaccg gtcaggtcgc gccgaacgag tccgatgcgg aggtccagca gcgcgcagcc
     8641 gatgccgatt cggtgatgcc gtggtccgcg gatgtacgca cgcaaacgga tctgccctcg
     8701 tcgactgctt ccgagcagac cgatgcggtg atcgttacgt caactgagtg ggtcgccacg
     8761 gaatctcacg gtgattcggt caccgtcatg catggccagg agacggtcgt taccccggtg
     8821 acctcccgcg agcccgagat ctctgcggac gcgcagacgt cctcgccggc cagggaagcg
     8881 gccggtgctg agaagagtga aggctttacc gggcccgatc gggcagacga ccgctccggc
     8941 gcggacgttc ccgccttcga gcccaccgcc gataccatgt ctggcacgat cgaatcgacg
     9001 acaccttcga gtgcgcccgg atcagtccag ggtcagccca gcgccgatgc aacgatcgtc
     9061 gaacagggcg atggattcgt acgcgtgcac gggatgagtg cctgcccgga gggcttcccg
     9121 atcaagggca atgccaacag cggcatctac cacgtgccgg ccgacgcgtc gtatcagcgc
     9181 accattcccg aggtctgctt cgcctccgag gacgtcgcaa tcgccaacgg ctaccgagcc
     9241 ccgaaaggcc gcggataacg cgcgcgacgg atcacaccga ttgacgtagc gcgggattac
     9301 catgccccgc gtcgttcgtc cccacgcacg gctgttagag ccggaccgca tccggggttg
     9361 gttcccccca tgccttggcc gtcgtcgggt tcgggcgggg cgtggtaacc ccgcgctacg
     9421 gatattggct cgaccgtcaa ctccacgtgt gtacaagcaa acgcgggaag cccgaatggg
     9481 ctccccgcgt ttcgttgcgg cgatcagacc ttcgccaatg cctggtcgag gtcgtcgatc
     9541 aggtcggaga ccgtctcgat gccgaccgag aggcgaacgt agtccggcgt gacaccggtc
     9601 agagtgtgct cctcctcggt gagctgcgag tgggtcgtgc tggccggatg gatgatcagc
     9661 gacttcgcgt cgccgatgtt ggcgaggtgc gagaagagct tgaccgactc gatcagcttg
     9721 cgaccggcct ctagtccgcc cttgacacca aagcccatga tcgcgccgaa cttcccgcgg
     9781 tagtggtact tcgatgcctg ggcataggcc gggtgtgtcg gcagacccgg gtaattgacc
     9841 cagctcacct tcgggtgctc ggtcaggaag cgagcgaccg cgacggcatt ctcgctgtgc
     9901 cgctccatgc ggacgggcaa cgtttcgatc ccctgcagga tcagccagga gttgaacggc
     9961 gagatcgccg cgccggtatc gcgcaggacc gagacgcgca gcttgatcgc ataggtgatc
    10021 gcgccgaacg tgtcccagaa cttcatgccg tggtagctcg gatccggatc ggtgagctcc
    10081 gggaacttgc cggatgccgc ccagtcgaac ttgccaccgt caacgacgat accggcgatg
    10141 ctcgtgccgt gaccgcccag gaacttggtc gccgagtgga tgacgatgtc cgcgccgtgc
    10201 ttgatcgggt tgaccaggta cgggctcgga atcgtgttgt cgatgatcag cggaattccg
    10261 ttgtcatgtg cgaccttggc gaccgcttcg atgtcaagcg tgatcaggtt cgggttgccg
    10321 accgtctcgg aatagacggc cttggtcttc ggcgtgatcg cggcagcgac agcttccggc
    10381 gtcggctcgc agaagatgac gttgatgccc atcttcggca gcgtgtactg gaagaggctg
    10441 taggtgccac cgtagagact ggtcgtcgag acgatgtcgt cgccggcgcg ggcgatgttc
    10501 aggattgccg ctgtctcggc cgcctgcccg gaggcaaagg ccagcgccgc tacgccgccc
    10561 tccagcgccg ccacacgttg ctcgaaggca tcggtcgttg ggttcatgat ccgagtgtag
    10621 atgttgccga actcctgcaa accgaacagg cgggcggcgt gctccggatc cttgaagacg
    10681 tacgacgtag tctgatacag cggcacggcc cgcgaaagcg tgtttggatc aggatcctgg
    10741 ccggcatgca gtgccagcgt ctcaaacccc aacggcttct cggtcgtgtc tgacatggtg
    10801 cgtgtttccc tttccgaccc gccgcgatgc agcggatcat ccatcctgaa gacgtatctg
    10861 caggcgccgt ggaggccggc gctggtgcat tcagtgtgtg tcaggcccct ccgccgaacc
    10921 gcgccaggaa gggagtcaga accgcggtca gctggtccca ctccttcagg aaggcgtcgt
    10981 gcccgtgggg cgaatcgagc tcgtcgtagc ggacctcctt gcccgcggcg gcagtccgcg
    11041 ccgcgaattg gcagatcgcg tccggcggga agagccagtc gctg
//
LOCUS       DATAOZ010000380         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7784902, whole genome shotgun sequence.
ACCESSION   DATAOZ010000380 DATAOZ010000000
VERSION     DATAOZ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7784902"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VHR64_09680"
     CDS             complement(<1..392)
                     /locus_tag="VHR64_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX3303140.1"
                     /translation="MADDLTYASLVELGLLLRAGEISSVDLTGHFLDRLETHGPALNA
                     VVTVTRERALREARQADEERAEGLDRGPLHGIPYGAKDLLATIDYPTTWGAEPFRYQH
                     FDHDADVVTRLREAGAVLIGKLSMVELAG"
     gene            complement(385..633)
                     /locus_tag="VHR64_09685"
     CDS             complement(385..633)
                     /locus_tag="VHR64_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303141.1"
                     /translation="MTSDSSQVGDGDRPAESKEAKRAIKRRLKSISHRFPDRFSDEQV
                     DQIRSRVARSVTLGANLAKVDLPNGTSPDFDPRAMSNG"
     gene            complement(794..1402)
                     /locus_tag="VHR64_09690"
     CDS             complement(794..1402)
                     /locus_tag="VHR64_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303142.1"
                     /translation="MPLHPHRRLSVPGARTGIALFMAALLLSGLTGFALRSAHAAETP
                     ALSAGCTALNAPGYDNTGTSTFAGTATFLAGETVVVAATPATGAPTAFDIYVDWAVAQ
                     SAGVPGTATWVVPANGDYTLYWSANVDEAAWTVSCEAPGQDPTPTPEPTATPTATPAP
                     SSCVPRGNENAERASQNGKENSCLSKDKTFNATSGKKPGKSK"
     gene            complement(1528..2430)
                     /locus_tag="VHR64_09695"
     CDS             complement(1528..2430)
                     /locus_tag="VHR64_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303143.1"
                     /translation="MRSRRLSRRTLLGGAAAATLAPRTYEFVAAQDTPSYQEEPVRWP
                     NLILPHARLLSYYGFPGVPQMGILGEHSPEELYPILLDQALAYEEADPSKPVVMAYEV
                     IASVAQRDPQADGSYLARISDDVVQEYVDFCEKRKLHLLLDMQYGRLTTEQELEAIAK
                     WLEYPFVHPALDPEFSIEEGETPGVELGSIDGSDVTFAQNWVADFCATRGLPPKILIV
                     HQFNVYSISNKDVIQPVDGVQFCLEVDGFGTPDMKLETYGVLTQNPIEFHGFKLWYSG
                     EDDPMMSESDVVNLDPSPDVIIYQ"
     gene            2543..>3167
                     /locus_tag="VHR64_09700"
     CDS             2543..>3167
                     /locus_tag="VHR64_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303144.1"
                     /translation="MDVLLGVAALVVLISVPTLLGLIYLELRQRRMFGPRSIITNERA
                     NNDTAAIAMSLQAELERLRSDVHGILTAVSSNMEQVREEIASREPVSIPAPIAQPLSE
                     PPRIDSDRSAAIVDLYSALSRLDVAFLAVTRPVLLPGEAFDGGDDLPTEALRWESWND
                     VGAAAYHFAEVFSERRIRLDPPTRDHLTGTIGSIRRCLTERLYPLLSD"
BASE COUNT          539 a          984 c         1060 g          584 t
ORIGIN      
        1 ccggccagct cgaccatcga gagcttgccg atcaggaccg cgccggcctc gcgcagccgg
       61 gtgacgacgt cggcgtcgtg gtcgaaatgc tggtatcgga acggctcggc gccccaggtc
      121 gtcgggtagt cgatcgtggc aagcaaatcc ttggcgccgt aggggatgcc gtggagtgga
      181 ccccggtcga ggccctccgc ccgctcctca tcagcctgcc gtgcctcgcg cagcgcccgc
      241 tcgcgcgtaa cggttacgac cgcgttcagg gccggaccgt gcgtttcgag acggtcgagg
      301 aagtgcccgg tcaggtcgac tgagctgatc tcgccggcgc ggagcaggag cccgagctcg
      361 accagggagg cgtacgtgag gtcgtcagcc atttgacatc gctcgggggt cgaaatctgg
      421 actggtccca ttcggcagat cgaccttggc caggttcgct ccgagcgtga cgctccgagc
      481 gacacgactg cgaatctggt cgacttgctc gtcggaaaat cggtcgggga agcggtgcga
      541 gatcgatttc aggcggcgct tgatcgcgcg tttcgcttct ttggattccg ctggtcgatc
      601 gccgtcgcca acctggctcg agtcagacgt catagggact ccctgtcgcg ggtgacaatc
      661 acttccgaca gggtacatga tcggcctgat cggcttgaat gcgccttcat gaggaagcag
      721 tcgcggagta cgcgaaccgg gcgtccaacg aactggacgc ccggtggcag gtggtatcga
      781 gttctcgaac cggctacttc gacttgccgg gcttcttgcc agacgtcgca ttgaaggtct
      841 tgtccttcga caggcagctg ttctccttgc cgttctggct ggcccgttcg gcattctcgt
      901 tgccgcgcgg cacgcagcta gaaggcgcgg gcgtcgcggt cggagttgcg gtgggctccg
      961 gggtcggcgt cggatcctgg cccggcgcct cgcagctcac ggtccaggcc gcctcatcaa
     1021 cgttcgccga ccagtacagc gtgtagtcac cgttcgcggg cacaacccag gtcgccgtcc
     1081 ccggaacgcc ggcggactgg gcaacggccc agtcaacgta gatgtcgaat gcggttggag
     1141 ccccggtcgc aggcgtggca gccacaacga ccgtctcacc ggcgaggaat gtagctgtgc
     1201 cggcaaaggt gctcgttcca gtgttgtcgt accccggcgc gttcagggcg gtgcacccgg
     1261 cggagagcgc cggcgtctcc gccgcgtggg cagagcgcag ggcgaaaccg gtcagacccg
     1321 aaagcaggag ggccgccatg aagagggcga ttccggtgcg ggcgccggga accgacaagc
     1381 gtcgatgtgg atgtagaggc attagggact cctccaaaca aatgcacatg gtttgtcaat
     1441 ggggcagtga ggacagccta ccaacgaaac ggcccggaat acaggtgatt ccgggacgtt
     1501 ggggtgctga ttcggagcgg gagcttctta ctggtagatg atcacatcgg gggatggatc
     1561 gaggttgacc acgtccgact cggacatcat cgggtcgtcc tcgccgctgt accagagctt
     1621 gaatccgtgg aattcgatgg gattctgggt gagcacgccg tacgtctcga gcttcatgtc
     1681 tggtgtgccg aatccgtcca cctcgaggca gaactgcacc ccatcgaccg gctggatgac
     1741 atccttgttc gagatcgaat agacgttgaa ctggtggacg atcaggatct tgggcggcag
     1801 gccacgggtc gcgcagaagt cagcaaccca attctgcgcg aatgtgacgt ccgatccatc
     1861 gatcgagccg agctcgacgc cgggcgtctc gccctcttcg atcgagaact ctggatcgag
     1921 ggccgggtgg acgaaggggt actccagcca cttggcaatc gcttccagct cctgctccgt
     1981 cgtcaaccgg ccgtactgca tgtccagcag cagatgcagc ttccgcttct cgcagaaatc
     2041 gacgtactcc tggaccacgt catcggagat gcgggccagg tagctgccgt ccgcctgcgg
     2101 atcgcgctgg gcgaccgagg cgatcacctc ataggccatc acgaccggct tggacgggtc
     2161 ggcctcctca taggcaaggg cctgatcgag caggatcggg tagagctcct ccggcgagtg
     2221 ctcgccgagg atgcccatct gcggcacgcc tgggaagccg taataggaga gcaaccgggc
     2281 atgcggcagg atcaggttcg gccagcggac cggctcctcc tggtacgagg gcgtgtcctg
     2341 ggccgcgacg aactcgtagg tgcgcggcgc aagggtggct gctgccgctc cacccaacag
     2401 cgtgcggcgc gacaatcgac gagacctcat tgcggtgtac ccttcccggt gcgtgtgcgg
     2461 atgttcgata gtaccaattc aatgcgcagt cgcttataag cgatgacgct cgggcaatgc
     2521 cttcagtttc caggggagca ggatggacgt tttgcttggt gttgcggcgc tcgtcgtgct
     2581 catctccgtg ccgacactgc tcgggttgat ctacctcgag ctgcgccagc ggcggatgtt
     2641 cggcccccgc tcaattatca ccaatgagcg ggcgaataac gacaccgccg cgattgcgat
     2701 gtctctccag gcggaactgg agcggctgcg gtccgatgtc cacgggatcc tgaccgccgt
     2761 ctcctccaat atggagcagg tccgcgagga gatcgcgagt cgggagccgg tcagcatacc
     2821 cgcaccgatc gcgcagccgc tctccgagcc gccgcggatc gactccgacc gcagcgccgc
     2881 gatcgtcgac ctctattcgg cgctctcgcg gctggatgtc gcctttctgg ccgtgacacg
     2941 cccggtgctg ctgccgggtg aagcgttcga cggtggggac gatctcccga ccgaggcgct
     3001 gcggtgggag agctggaacg atgtcggcgc ggcagcgtac cacttcgccg aggtcttctc
     3061 ggagcggcgg atccggctgg atcccccgac gcgagatcac ctgaccggca cgatcggctc
     3121 gatccggcgc tgcctgaccg agcggctcta tcccctgctc tccgatt
//
LOCUS       DATAOZ010000381         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4891892, whole genome shotgun sequence.
ACCESSION   DATAOZ010000381 DATAOZ010000000
VERSION     DATAOZ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4891892"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1442)
                     /locus_tag="VHR64_09705"
     CDS             complement(93..1442)
                     /locus_tag="VHR64_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303145.1"
                     /translation="MLDWLRTDVPRLRWFADKTRPVADVDIAWTSTLPGTPEVRLTVA
                     RYRFDTGPDSLYFIPIVSDNGGDASAHPAFTAWLIDSLSDRAEAPGGWSWTRFPNART
                     ASLASREGRLLGAEQSNTSIRFGDDALIKLNRRLVVGPSPEAELAAVIAKAADASFAA
                     QSYGVLNLAGLADEPISVAIAAEYVPNVGDAWTWLLALLTDPDDPSGAVATEIERIAD
                     VTATMHIGLTSDPWRRAVAPEPIKPDDVAAWESAGLDALGMLEQTIANLESTLQPDAL
                     RLARLLPTAAAVLREQIHGFDALRGTYRIRIHGDYHLGQLLRKPDGAYVVVVFDGEPN
                     RPLHERRDKYSALRDVAGMLRSFAYARGTAERSDPIEGRSAAWLSWENRARDLYIRRY
                     LDRVAGQSTALVPGSLEDTRIALAALELEKAVYECGYELGNRPDWLWIPLSRLVRAG"
     gene            complement(1452..3098)
                     /gene="treS"
                     /locus_tag="VHR64_09710"
     CDS             complement(1452..3098)
                     /gene="treS"
                     /locus_tag="VHR64_09710"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX3303146.1"
                     /translation="MADQQQDPLWYKDAVFYELHARSFFDSDNNGKGDFAGMAMRLDY
                     VRDLGVDCIWIQPFYPSPLLDDGYDIADFTGIHPDFGTLEDFRRLVEAAHERGLKVLV
                     DFIMNHCSDLHAWFQEARHDRNSPKRDYFVWSDDPTRYGDARIIFIDTQPSNWTFDEV
                     AQQYYWHRFFEHQPDINYDNPAIWDEMFRIARFWLDLGIDGFRCDAIPYLYERENTNC
                     ENLAETHEFLRAFRAMIDREYPGKLLLAEANQWPGDVVHYFGTEHQPEFQMGFHFPVM
                     PRLFLSLRREEPSSVIQILRATPEIPADTQWCIFLRNHDELTLEMVTEDEREEMWFEY
                     APEPRMRCNLGIRRRLAPLVDNDRSQLELLNALLFSLPGSPIVYYGDEIGMGDNIWLR
                     DRNGVRTPMQWSADENAGFSSAHPGVLFAPVIDEGEFSYRTVNVANQDRNPTSLLNWM
                     RKIIAIRKQVKAFGRGSFTILDVDNPHIFAFERKHENERVLCVFNFSQAMQSVAVPLK
                     VTERRQVRDMIGGSTLHDARTRGKYQITLGPRGYAWLCVS"
     gene            complement(3114..>3707)
                     /locus_tag="VHR64_09715"
     CDS             complement(3114..>3707)
                     /locus_tag="VHR64_09715"
                     /inference="COORDINATES: protein motif:HMM:NF044853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX3303147.1"
                     /translation="SQYMRGNLFVNTHDINPFHLQTGGRPAFRTRLVLAATLSSVYGI
                     YSGFELCEATPVSGKEEYLNSEKYEYKVWDWDRPGNIKDLVRIVNNARKDHPALQEYD
                     NLRFFSADDERVMVYAKVSNDGSDRIVCIVNLDPHRTVSTWINLDLSQLGLTDGITYE
                     VTELITGNHWSWTGAHHHIQINPMIEPAWILSLSPLD"
BASE COUNT          698 a         1144 c         1159 g          706 t
ORIGIN      
        1 cgccctggtg cccacgagca gctgaccggg cgagccgccc ggcactacac cggcactaca
       61 tcagaagtaa cgctcttctt gcgaaccgcg cgctaccccg cccggaccag cctggagagc
      121 ggtatccaga gccagtccgg ccgattgccg agctcatacc cgcactcgta gaccgccttc
      181 tcgagctcga gcgcggccaa cgcgatcctc gtgtcttcga gactcccagg cacgagcgct
      241 gtcgactgcc ctgccacgcg atccaggtag cgccggatgt acaggtcccg agcccggttc
      301 tcccatgaca gccaggctgc actccggccc tcgatcggat cggaccgctc agcagtgccg
      361 cgcgcgtatg caaacgagcg cagcatgcca gcgacgtccc gaagcgccga gtacttgtct
      421 cgtcgctcat gcagcggccg gttcggttcg ccgtcgaaaa cgacgaccac gtatgcccca
      481 tcgggtttgc gcaggagttg ccccagatgg taatcgccgt ggatccggat ccgatacgtg
      541 ccgcgcagcg cgtcgaaacc gtgaatctgc tcgcgcagga ccgcggccgc agtcgggagc
      601 agccgggcga gccgcaatgc gtccggctgg agcgtcgatt ccaggttggc gatcgtctgc
      661 tccagcatcc cgagggcatc gagtcccgcg ctctcccacg cggcgacatc gtctggtttg
      721 atcggttccg gcgcgacggc acgccgccag ggatcgctgg tcaggccgat gtgcattgtt
      781 gccgtcacgt cggcgatccg ctcgatttcg gtcgcgaccg cgccggacgg atcatcagga
      841 tcagtcagga gcgcgaggag ccaggtccag gcgtctccaa cattcggcac gtattcggcg
      901 gcgattgcga ccgatattgg ctcgtcggcg agtcccgcca gattcaggac accgtatgac
      961 tgtgccgcaa acgacgcatc ggccgccttc gcgatcactg cggccaactc ggcctccggt
     1021 gatgggccga ctaccaggcg gcgattgagc ttgatcaacg cgtcatcccc gaatcggatc
     1081 gacgtgtttg attgctccgc cccgagcaag cgtccttctc gggatgcgag cgacgcggtc
     1141 ctggcgttcg ggaagcgggt ccacgaccag ccgcccggcg cctcggccct gtccgaaagc
     1201 gaatcgatca gccacgcggt gaaggcagga tgcgcggacg catccccacc gttgtcgctc
     1261 actatcggga tgaagtaaag gctgtccggt ccggtatcaa agcgataacg cgcgaccgtc
     1321 agccggactt ctggtgtgcc cggcaacgtt gacgtccacg cgatgtcgac atctgccacc
     1381 ggccgcgtct tgtcggcgaa ccaccgcagg cgcggaacat cggtgcgcag ccagtcgagc
     1441 atcagccgtc gtcagctcac gcacagccac gcgtacccgc gcgggccgag tgtgatctgg
     1501 tatttgccgc gcgtgcgggc gtcgtgaagc gtactcccgc cgatcatgtc gcggacctga
     1561 cggcgctcag tgaccttgag cggcacggcg acgctctgca tggcttgtga gaagttgaag
     1621 acacacagca cgcgctcgtt ctcgtgtttc cgctcgaagg cgaagatgtg aggattgtcg
     1681 acgtccagga tcgtgaagct gccgcggccg aacgctttga cctgcttgcg gatcgcgatg
     1741 atcttgcgca tccagttgag gagcgacgtc ggattccggt cctggttcgc gacattgacg
     1801 gtccgatagc tgaactcgcc ttcgtctatc accggcgcga agagaacgcc tggatgggcc
     1861 gatgagaagc cggcgttctc gtcggccgac cactgcatcg gcgtgcgaac cccattccgg
     1921 tcacgcagcc agatgttgtc gcccatgccg atctcgtcgc cgtaatagac gattggcgac
     1981 ccgggcaacg agaagagcag cgcattcagc agttcgagct gggatcggtc gttatccacc
     2041 agcggagcga ggcggcggcg aatgccaagg ttgcagcgca tgcgtggctc gggcgcgtac
     2101 tcgaaccaca tctcctcgcg ctcgtcttcg gtaaccatct cgagcgtcag ctcatcatgg
     2161 ttacgcagga agatgcacca ctgggtgtcg gccgggatct ccggcgtcgc tcgcagaatc
     2221 tggatgaccg atgacggctc ctcgcgcctg aggctcagga acagccgcgg catgaccgga
     2281 aagtggaatc ccatctggaa ttctggctgg tgctcggtgc cgaagtagtg gacgacgtcg
     2341 cctggccact gatttgcctc agccaggagc aacttgccgg ggtattcgcg atcaatcatc
     2401 gcccggaagg cgcgcaggaa ctcatgcgtc tcggcaaggt tttcgcagtt ggtgttctcg
     2461 cgctcataca ggtatgggat cgcgtcgcac cgaaacccgt cgatgccgag atcgagccag
     2521 aatcgcgcga tccggaacat ctcatcccag atcgctgggt tgtcataatt gatgtccggc
     2581 tgatgctcga agaagcggtg ccagtagtac tgctgcgcga cctcatcgaa cgtccagttc
     2641 gagggctggg tgtcgatgaa gatgatccgc gcatcgccgt acctggtcgg atcgtcggac
     2701 cagacgaagt agtcccgctt gggcgagttg cgatcgtgtc gggcttcctg aaaccacgca
     2761 tggaggtcag agcagtggtt catgatgaag tcgacgagca ccttcagccc gcgctcgtgc
     2821 gctgcttcta ccaagcggcg gaagtcttct agcgtgccaa agtccgggtg gattcccgtg
     2881 aagtcggcga tgtcgtagcc gtcgtcgagc aggggagagg gatagaacgg ctggatccag
     2941 atgcaatcga cgccgaggtc gcgcacgtag tcgaggcgca tcgccattcc ggcgaaatcg
     3001 cccttgccat tgttgtccga gtcgaagaat gatcgcgcgt ggagctcgta aaagaccgcg
     3061 tccttgtacc acaaggggtc ctgctgctgg tccgccaaac ccgattccct ctatcaatcc
     3121 aaaggtgaaa gcgacaagat ccacgctggc tcaatcatag ggttgatttg gatgtggtga
     3181 tgtgcgccgg tccacgacca gtgattgccg gtaataagct cagtaacttc ataggtgatg
     3241 ccatcggtaa gtccgagctg cgagagatcg agattgatcc aggtgctgac ggtgcggtgc
     3301 ggatccagat tcacgatgca gacgatccga tcggagccgt cgttcgacac cttcgcgtag
     3361 accatcacgc gctcgtcatc tgctgagaag aatcgcagat tgtcgtactc ctggagggcg
     3421 ggatggtcct tgcgcgcatt attgacgatg cgcaccagat ccttgatgtt gcccggccga
     3481 tcccaatccc agaccttgta ctcgtacttt tcggagttga ggtactcctc cttgcctgat
     3541 acaggagtcg cctcgcaaag ctcgaagccc gaatagatcc cgtacaccga tgaaagtgtc
     3601 gcggccagta ccagtcgcgt ccggaatgcg ggacgccccc cggtctgcag gtggaacggg
     3661 ttgatgtcgt gagtgttgac gaagagattg ccacgcatgt actggct
//
LOCUS       DATAOZ010000382         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3313301, whole genome shotgun sequence.
ACCESSION   DATAOZ010000382 DATAOZ010000000
VERSION     DATAOZ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313301"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1014
                     /locus_tag="VHR64_09720"
     CDS             67..1014
                     /locus_tag="VHR64_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3303148.1"
                     /translation="MASEQTIGGALQRPDLATARSGRRSGAPDGAFRYGMTLPTVLAL
                     LLVVAFPLAFALYVSTHEYDLTKGGIGAFTGIENYARTISDEQIGNAARNTVILSVSV
                     VAIELVVALGLSVLLNQPGLRFRNVYLAILLIPLLISPVAVGLIWRLLLHADLGAVNW
                     ALGLVGLPKQEWLSGQTTALPTIVGVDVWHETSLMIVIILAGLTALPRDPIEAAAVDG
                     ANRWQAFWTVTLPLLMPVLLVAVLIRMIAAMKTYDLIYILTRGGPGSATETISYSIWK
                     NAFTSLDMGRSAAASFLLLVVILALTVVLVRVMDVGRDV"
     gene            1097..1684
                     /locus_tag="VHR64_09725"
     CDS             1097..1684
                     /locus_tag="VHR64_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303149.1"
                     /translation="MAREDTGTEQNGDPDRELSSVQPSAGAAATTGSVASEVIVNAGP
                     ENHPTIATEILRAEAGDVQATTVTLDHAGAEQVTAERIVMINSGARTVGARSAQVDRS
                     GILVVRSDKAVFSNSTAIAIATEEARIVRSRVVMLKADRATIEAGAKVVIYAGPAGEN
                     MRPLADVRGAAAFGAGLGAVLLLLGPFLRRILRLR"
BASE COUNT          303 a          546 c          673 g          356 t
ORIGIN      
        1 ccgcagcgga gttgaaagag ctgttgtagg gggatgggtg acggatgatg ggtgacagct
       61 cagtccatgg ctagcgaaca gacaatcggg ggtgcgctgc aacggccgga tcttgcgaca
      121 gcgagatccg gccggcgaag cggcgccccc gacggcgcgt tccgctatgg gatgacgttg
      181 cccacggtgc tggcgctgct cctggtggtg gcgtttcccc tggcgtttgc gctctatgtc
      241 tcgacgcacg aatacgacct aactaagggt ggaatcggtg catttacggg cattgagaac
      301 tacgcgcgga ccattagcga tgagcagatc gggaatgcgg ccaggaatac ggtcatcctc
      361 tcggtcagcg tcgtcgcgat cgagttggtg gtcgcgctgg ggctttcggt gctactcaac
      421 caacccggcc tgcgcttccg taatgtctac ctggctatcc tgctcattcc gctattaatc
      481 agtccggtcg cggtcgggct gatctggcgg ctcctcctgc acgcagatct gggcgcggtg
      541 aactgggcgc tgggactggt tgggctgccg aagcaggagt ggctgtccgg gcaaacgacc
      601 gcgctgccga cgatcgtcgg agtcgacgtc tggcatgaga cgtcgctgat gatcgtcatc
      661 atcctggctg ggctgaccgc gttaccgcgt gatccgatcg aggcggcagc agtcgacggg
      721 gcgaaccgct ggcaggcgtt ctggacggta acgctgccgc tgctgatgcc ggtgctgctg
      781 gtggcggtgc tgatccgcat gatcgccgcc atgaaaacgt acgacctgat ctacatcctg
      841 acccgcggtg ggccgggtag cgccacggag acgatctcct attcgatctg gaaaaacgcg
      901 ttcacctcgc tcgatatggg ccgctccgcc gccgcgtcgt ttctgctgct ggttgtcatc
      961 ctcgcgctga ccgtcgtgct cgtgcgcgtg atggatgtcg gtcgcgacgt ctgatctgct
     1021 gccgcggcca gcaggggaag acccgatgct acactcgcct gcctgatcgg ggattgatgc
     1081 gagaagggcg gccagcgtgg cgcgggagga caccgggacg gagcagaatg gcgatcccga
     1141 tcgcgagctg agctcggtgc agccgtccgc tggggctgcg gcgaccactg ggtccgtggc
     1201 gtccgaggtc atcgtgaacg cgggtcccga gaaccatccg acaatcgcca ccgagattct
     1261 tcgggccgaa gcgggcgacg tccaggccac gacggtgaca ttggaccacg ctggagccga
     1321 gcaggtgacg gcggagcgga tcgtcatgat caactccggt gcgaggactg tcggggcccg
     1381 ttccgctcag gtcgaccgct ccggtatcct cgtcgtgcgc agcgataagg ccgtcttctc
     1441 caactcgacc gcgatcgcga tcgccactga agaggcgcgg atcgtgcgaa gtcgagtcgt
     1501 gatgctcaag gccgaccggg ccacgatcga agccggggca aaggttgtga tctacgccgg
     1561 gccggccggt gagaatatgc gtccgctggc cgatgtccgt ggagccgccg cattcggcgc
     1621 tgggttgggc gctgtgctgt tgttgcttgg cccttttttg cgccggatct tgcggctgag
     1681 atgagccgtc gctggagggc gtcatgggac tgataaagag actcgggtcg ttcatcgctg
     1741 ggggactgct cggcgccggg gtcggcgcgg cagtcgccat gatcgtggcc ccgcagagcg
     1801 gcgacgagtt cgtcgaaggg gtcgagcgta gggtcgatct ggcgaagcga gccgggttgg
     1861 aggcgcaggc ccgcacag
//
LOCUS       DATAOZ010000383         9766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6205879, whole genome shotgun sequence.
ACCESSION   DATAOZ010000383 DATAOZ010000000
VERSION     DATAOZ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6205879"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /gene="mreD"
                     /locus_tag="VHR64_09730"
     CDS             complement(<1..407)
                     /gene="mreD"
                     /locus_tag="VHR64_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX3303150.1"
                     /translation="MNRLFYALVMFATAIFQATVIGMYSPIEIQPDITLVLLLVWCAN
                     TGTAEGLTWAFGLGILIDVLALDALGTNPLALLPVALLAAASRKRLFQSKLLFPILLT
                     VAATMFHAVVLLLLRSGAVGDVPLATVFRLVFL"
     gene            complement(426..1154)
                     /locus_tag="VHR64_09735"
     CDS             complement(426..1154)
                     /locus_tag="VHR64_09735"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX3303151.1"
                     /translation="MSMSFTIGWLYPSKMNIYGDRGNVIALKQRAAWRGLCAKVVEIN
                     IGDPIPDDIDVFFFGGGQDEQQVAVSRDLQGAKGEAIKTAIENGAGMLAVCGGYQLLG
                     HEYRPHATDPLPGIGLFDVVSVAGPERFIGNVVVESQWGDLVGFENHSGLTNLGVGQQ
                     PMGRVKVGRGNNGSDGTEGAIYRNAIGCYLHGSLLPKNPAVTDWLIKQSLRRRDPDFT
                     LPPLSDAFEQAARSPAIERAVATR"
     gene            complement(1157..2569)
                     /locus_tag="VHR64_09740"
     CDS             complement(1157..2569)
                     /locus_tag="VHR64_09740"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX3303152.1"
                     /translation="MNKTIDMRLGLAAAAAKLTTAGLRRTGRGGGTAAPGLVADFVDP
                     GILRKIGLRLDRGVIVVAGTNGKTTTSRMIADMLEADGSRVVHNRSGSNLVRGVAAAF
                     AEQTTWRGEPNGDVGVVESDEAAFSAIVERLQPKVILLNNLFRDQLDRYGELDTIARK
                     WSATLSRLPASTTVVVNADDPTLAELTRDIPARRITFGMNERRYVLDHLPHAADSGVC
                     RRCGADLTYTALYVSHLGSWRCPRCTAARPPLDIIGSEIALKDVESLELTVFRQGAEP
                     IRIAAGVPGMYNAYNVLATTAVAAALGVQPPVVQRAMAAFKSAFGRIERVEIAGRTIT
                     LALVKNPVGFNEVLRMLTMATDGLSVPTMIVINDRHADGRDVSWLWDVDFELLADGDA
                     PLTTAGIRGTDMANRLKYAGVSDARIRSLGPDPGAAVDQFVTSIPEGGRGYILATYTA
                     MLDLRANLAERGAVERFWEQ"
     gene            complement(2574..3398)
                     /gene="mreC"
                     /locus_tag="VHR64_09745"
     CDS             complement(2574..3398)
                     /gene="mreC"
                     /locus_tag="VHR64_09745"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX3303153.1"
                     /translation="MGSLSARQTAMLILLFVVTSVAFIVLDNQSALNPLKTGLRDLVN
                     PITERAFDRGEPKKTTEGEWEAKYHDLEDQYAQLDAEYQRTKIVADQVQALQAMLNLQ
                     KTSPEVTFLPARVVSVDPTGVNKIIVIDRGSNDGIAVGMAVTDPNYYVGVVVQVDDTS
                     SQVLLAIDSTHTVGVELESSHATGVAYGMWQQGGRMEIRYVDRNVEVKPEEKVITTCA
                     PDIRTANMPCGLIIGKASGQPTIDSQGDTQTVQVIPAADFDNLSFVTVITSTGQGQ"
     gene            complement(3453..4511)
                     /locus_tag="VHR64_09750"
     CDS             complement(3453..4511)
                     /locus_tag="VHR64_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX3303154.1"
                     /translation="MIRPLSTLFGFFSRDLGIDLGTANTLVYVRGKGIVISEPSVVAI
                     DTKTKRALAVGVEGKKMVGKTPESIIAVRPLKDGVIADFDTVEMMLHYFIRKVHMQRM
                     MAPHPRVVVGIPSGVTEVEKRAAKDAALSAGAREAYTIEEPMAAAIGAGLPINEPVGS
                     MIVDIGGGTTEVAVISLGGIVINHSIRVAGDEIDDAIIQYARRDHNLVIGERTAENAK
                     IAAASAYPLEEEIRVVLRGRDLLTGLPKSVEISSVELREAISPPVNTIVELVKTAIEE
                     TPPELVADIMEQGITLAGGGALLHGLDRRLQNELKMPVIRAEDPLTCVARGTGRVAEA
                     LHLYERALAGGRELRRTS"
     gene            complement(4688..4945)
                     /locus_tag="VHR64_09755"
     CDS             complement(4688..4945)
                     /locus_tag="VHR64_09755"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX3303155.1"
                     /translation="MNPILWIIFGAITGWIAGRLYGDDRPEGCVTNTVLGIGGALLGG
                     AIYTLITGRDFTAGFNLTSLVVAILGALLLIFLLRRLGRFG"
     gene            5024..5695
                     /locus_tag="VHR64_09760"
     CDS             5024..5695
                     /locus_tag="VHR64_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303156.1"
                     /translation="MVFRYRFSVYRFPAARLLASRLLAFFSTSRLLDYTPAMDMNLRH
                     LAGGELTDSLPETPHELPFDRGKIDELIDRVRQGEPVDLLTEMLACVDWRGSFTEPDG
                     RPITIEDIARLIAYYKQKFADVGVIYIADLLSTEFMTEQRARGDVVFSDDLLRLFREQ
                     PEVWQEIRLFFRRKEFVTALLTQSHASRWASLNPDARRGTSDASQKTSDFSGQMSEMG
                     TADQG"
     gene            complement(5739..6182)
                     /locus_tag="VHR64_09765"
     CDS             complement(5739..6182)
                     /locus_tag="VHR64_09765"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX3303157.1"
                     /translation="MEVSGEHRFDVPREKVWQFLLDPDVLKASLPGCEKLEEVGPDEY
                     VATMKIGISFIRGTFTGKVKISDKNEPESYKMSVEGSGPQGQVSGVGNLELVEDNGGT
                     IVKYKGDANVRGSLARVGARMIQPAAKTIVGQFFDKMEKEAAARA"
     gene            6465..7352
                     /locus_tag="VHR64_09770"
     CDS             6465..7352
                     /locus_tag="VHR64_09770"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX3303158.1"
                     /translation="MVSSRPLAPTSTSPLRSLRRVSDRHDSFDLDTDPVFDPNAQRIA
                     TADLGRIAPDTRRRGSLKTSGVLPRHTGYLHGVSLSAILQMLHLERKTCVVEVSAHGL
                     LGSLILVNGELVDASAGDLSGEEAACEILGWIDPQTAILAGAETFRHTVQRPITQLIM
                     DAVRIVDESGMLNPASLPEFDRNPGARVSGDWQWLIDSLSMVGATNVQIVTPDSLPSE
                     RRQTGFLGGDPAADLARGIRTWATLLGPDVTEVVATRSDCIVVLAVLDADRSEFVYAE
                     APGLETAELVRRSLRSIRK"
     gene            7479..8912
                     /locus_tag="VHR64_09775"
     CDS             7479..8912
                     /locus_tag="VHR64_09775"
                     /inference="COORDINATES: protein motif:HMM:NF004801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase family protein"
                     /protein_id="HEX3303159.1"
                     /translation="MATRTRINAAHIIATQNNEHRHLRDGVVVYEGDRIVHVGKSFDG
                     NADQTIDLPHGVVTPGFINTHAHLAGSPLDKSFIEDVGPRQFYGSGLFEYLPVRGGAQ
                     DAEADIACLAFSMTELMRTGTTTIMEIGHRAPEAVEEADKVGMRLYMGLGYRSGKWLT
                     TDGKRVSWEWDEPAGYAGMERAIKFIEEYDGKSNGRIKGFLSPMQIDTCTEDLLRKSK
                     DAADSMGVPIALHAAQSVNEFNEMVARHGRSPIEWLEDINFLDSNVILGHAIILAGGT
                     WANYAGNDIEILARTHANVAHCVWVFARRGIAMESFARYTEAGVNMTLGTDTAPQSML
                     EALRWAAVVSKLMDRQTGVATAADVFKAATLNGAKALNRDDLGRIEVGAKADLLIWDG
                     DTIFMSPLRDPIKNIVYSAEAEDLNTVIIDGEIRMKDRQVLGVDTRKLALDLQAAGER
                     MWPEMAQYDWNGRDADKLSPQSFPEWK"
     gene            9035..9355
                     /locus_tag="VHR64_09780"
     CDS             9035..9355
                     /locus_tag="VHR64_09780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3303160.1"
                     /translation="MPDLGPVVDNVNEQQFELTVDGLTAFAAYQREGDVIVFTHTEVP
                     TEHQGKGVGGALARGALDAARAEGAEVIPLCPFIASYIRRHPDYLNLVSERNQRRLKL
                     QASQ"
     gene            complement(9377..>9766)
                     /locus_tag="VHR64_09785"
     CDS             complement(9377..>9766)
                     /locus_tag="VHR64_09785"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX3303161.1"
                     /translation="NGSGVLGTHAAAQGTDAVAAGNLLRDESIPNEMIAAFEANPEAE
                     LGDRLIAALQAAVACGGEMGPVHSAGMLIAGDVEWPITDLRVDWSDDPIAHLAALWRL
                     WRPQAQDYVNRAINPTLAPTYGVPGDE"
BASE COUNT         1716 a         3167 c         3137 g         1746 t
ORIGIN      
        1 tgcaagaaaa ccagccgaaa cacggtggcc aagggcacat cgcccaccgc accgctgcgg
       61 agcaagagca agactaccgc gtggaacatc gttgccgcca ccgtaagcag gatcgggaag
      121 agcaacttgc tctggaataa ccgcttgcgc gaggcagccg ccaggagcgc gaccgggagc
      181 agggccagcg gattcgtgcc gagggcatcg agcgcgagca catcgatcag gatgccgagg
      241 ccgaacgccc aggtgagacc ctcggcagta ccggtattgg cgcaccagac aaggagcaat
      301 acgagcgtga tatccggctg gatctctatc gggctgtaca tgccgatcac ggtcgcctgg
      361 aagatcgccg tggcgaacat gacaagcgcg tagaacagcc ggttcattcc cccagtcccc
      421 gccgcttacc gggttgcgac ggcgcgctcg atcgccgggg accgggccgc ctgctcgaat
      481 gcgtcggaga ggggcggaag cgtgaagtcg ggatcgcgcc ggcgcagcga ctgcttgatc
      541 agccagtcgg tcaccgccgg gttcttgggc agcagcgagc cgtgcaggta gcagccgatc
      601 gcgttccggt aaatcgcgcc ctcggtgccg tcggaaccgt tgttgccccg cccgaccttg
      661 accctgccca tcggctgctg gccaacgccg aggttggtca ggccggagtg gttctcgaag
      721 ccgaccagat cgccccactg gctctcgacc acgacgttgc cgatgaaccg ctccggtccg
      781 gcgacgctga cgacatcgaa caggccgatc ccgggcagcg gatcggtcgc gtgcgggcgg
      841 tactcatgac cgaggagttg atagccgccg cagacggcca gcatcccggc gccattctcg
      901 atggcggttt tgatcgcttc gcccttggca ccctggagat cgcgcgagac tgcgacctgc
      961 tgctcgtcct gaccgccgcc gaagaagaag acgtcgatat cgtcagggat cggatcgccg
     1021 atgttgatct cgaccacctt cgcgcagaga ccgcgccagg ccgcccgctg cttgagcgcg
     1081 atcacgttgc cgcggtcgcc gtagatgttc atcttgctcg ggtagagcca gccgatcgtg
     1141 aaagacatgc tcattgttac tgctcccaga agcgctcgac ggcgccccgc tcggcgagat
     1201 tggcgcggag atcgagcatg gctgtatagg tcgcgaggat gtagccacgc ccgccttcgg
     1261 ggatgctcgt gacgaactga tccaccgccg caccgggatc gggaccgagg gatcggatgc
     1321 gggcatctga cacgccggcg tacttgagcc ggttggccat gtccgtgccg cggatgccgg
     1381 cggtcgtcag cggcgcatcg ccatcggcca gcagctcgaa atcgacgtcc cacaaccacg
     1441 agacatcacg cccgtcggcg tgacggtcgt tgatcacgat catggtcggg acgctcaggc
     1501 cgtccgtcgc catggtcaac atgcggagca cttcgttgaa gccgaccggg ttcttgacca
     1561 gcgcgagggt gatcgtgcga ccggcgatct cgacccgctc gatgcgtccg aacgccgact
     1621 tgaatgcggc cattgcgcgc tggacgaccg gaggctgcac cccgagcgcg gcggcgacgg
     1681 cggtcgtggc aagcacgttg taggcgttgt acatgccggg cacgccggcg gcgatccgga
     1741 tcggctccgc gccttgacgg aacacggtca gctcgagcga ctcgacgtcc ttgagcgcga
     1801 tctcgctgcc gatgatgtcg agcggcggcc gcgcggccgt gcagcgcggg cagcgccaac
     1861 tgccgaggtg cgagacgtag agcgcggtgt acgtgagatc ggcgccgcag cggcggcaga
     1921 cgccggagtc ggccgcgtgc gggaggtgat cgaggacgta gcggcgctcg ttcatgccga
     1981 aggtgatgcg gcgggccggg atatcacgcg tcagctcggc gagagtcggg tcgtcggcgt
     2041 tgacgacgac ggtcgtcgaa gcgggcaggc gagacagggt tgccgaccac ttccgggcga
     2101 tcgtgtccag ctcgccgtag cgatcgagct gatcgcggaa gaggttgttg agcaggatca
     2161 ccttcggctg cagccgctcg acgatcgcgc tgaaggccgc ctcgtccgac tcgacgacgc
     2221 cgacatcgcc attcggctcg ccacgccagg tcgtctgctc ggcgaaggcc gccgccacgc
     2281 cgcgaaccag gttcgagccg gagcggttgt ggacgacccg cgagccgtcg gcctcgagca
     2341 tgtcggcgat catccgggag gtcgtggtct tgccgttggt cccggccacg acgatcacgc
     2401 cgcggtccaa ccggaggccg atcttgcgca ggatgccggg gtcgacgaaa tcggcgacca
     2461 gcccgggtgc ggcagttccc ccgccgcgac cggtccggcg cagtccggcg gtggtcaact
     2521 tggccgcggc ggcggccaac ccgaggcgca tgtcaatcgt cttgttcaat ctgctactgc
     2581 ccctgccccg tcgaggtgat cacggtcacg aacgacaagt tgtcgaagtc ggccgccggg
     2641 atcacctgca ccgtttgcgt atcgccctga ctatcgatcg ttggttgccc ggatgccttg
     2701 ccaatgatca gcccgcacgg catgttggcg gtgcggatat ctggcgcgca ggtcgtgatg
     2761 accttctcct cgggcttgac ctcgacattg cggtcgacgt agcggatctc catccggccg
     2821 ccctgctgcc acatgccgta ggccacgccg gtcgcatggc tgctctccag ctcgacgccg
     2881 acggtgtggg tcgagtcgat cgcgaggagt acctgtgatg aggtgtcgtc gacctggacg
     2941 accacgccga cgtagtagtt cggatcggtg acggccattc cgaccgcgat gccgtcgttc
     3001 gagccgcggt cgatcacgat gatcttgttg actccggtgg gatcgaccga gaccacgcgc
     3061 gcaggcagga aggtcacttc ggggctcgtc ttttgcaggt tcagcatcgc ctggagggcc
     3121 tgaacctggt ccgcgacgat tttggtgcgc tggtattcgg catcaagctg ggcgtactgg
     3181 tcctcgaggt cgtggtactt ggcttcccac tcgccctcgg tcgttttctt cggttcgccg
     3241 cgatcgaagg cgcgctcggt gatcgggttg acgagatcgc ggaggccggt cttgagcgga
     3301 ttgagggcgg attgattgtc gaggacgatg aaggcgacgc tggttacaac aaacagcaaa
     3361 atcaacatcg cagtctgtcg ggcactcaac gatcccatgc ttcagcctca accgcggggg
     3421 tatagctcga attggaccgc tcgggacggt ccctaactgg tgcggcgcag ctctcggccg
     3481 ccggccagcg cgcgctcata gagatggagc gcttcggcca cgcgtccggt tccgcgggcg
     3541 acacatgtca gcggatcctc agcacggatc accggcatct tgagttcgtt ctgcaagcgc
     3601 cgatccagac cgtgcaacag cgcgccaccg ccggcgagcg taatgccctg ctccatgatg
     3661 tccgccacca gctccggcgg cgtctcctcg atggccgtct tgaccagctc aacgatcgtg
     3721 ttgacgggcg gcgagatcgc ctctcgcagc tcgaccgagc tgatctcgac cgatttcggc
     3781 aagccggtca acaggtcccg accgcgcagg acgacccgga tctcttcttc gagggggtag
     3841 gccgaggcag ccgcgatctt cgcgttctcc gccgtgcgct cgccgatgac gaggttatgg
     3901 tcccgccggg cgtactggat gatcgcatcg tcgatctcat ccccggccac gcgaatcgag
     3961 tggttgataa cgataccgcc gagcgagatg acggcgacct cggtcgtgcc accgccgatg
     4021 tcgacgatca tgctgccaac cggttcgttg atcggcaagc cggcgccgat cgccgcggcc
     4081 atcggctctt cgatcgtata ggcctcgcgg gcgccggcgg aaagggcggc atccttggcc
     4141 gcgcgcttct cgacttcggt cacaccgctc gggatgccga ctacgacgcg cgggtgcggc
     4201 gccatcatcc gctgcatgtg caccttgcgg atgaagtagt gcagcatcat ctcgaccgtg
     4261 tcgaagtcgg cgattacgcc gtctttgagc ggtcgcaccg cgatgatgct ttccggcgtc
     4321 ttgccgacca tcttcttacc ctcaacgccg acggccaacg cgcgcttggt cttggtgtcg
     4381 atcgcgacaa ccgacggctc ggagatgacg atccccttgc cacggacata gaccagggtg
     4441 ttggcagtgc cgaggtcaat gccaagatcc cggctgaaga agccgaagag ggtcgagagt
     4501 gggcgaatca agagggggaa cctcccaggc gtcaagcgtc gggacgatta ccgacgagtc
     4561 tgccgcaagt tgcagggttc agtgactgtt cgatcgaggg gcagagaata cgccaccgtg
     4621 tgcgacataa cgcagccgga aaccgcatcg cagtatagca cagcgacctc tcccgaacgg
     4681 gtcccggtca cccgaatcga ccgagtcgcc ggagcaggaa aatcagcagc agtgcgccaa
     4741 gaattgcgac cacaagactc gtcaaattga agccggcggt gaagtcgcgc ccggttatca
     4801 gcgtgtagat cgccccgccc agcaaagcgc cgccgatacc gagcacggta ttagtcacgc
     4861 atccctccgg tcgatcatcc ccgtacaggc ggccggcgat ccaaccggtg atggcgccga
     4921 agatgatcca gaggatgggg ttcatgcgtt gctccagtgg tcagtggacg gagtttcttg
     4981 tttctggttt ccagtttcca gttgggtctt tggtctctcg tctttggtct ttcgatatag
     5041 gttttccgtt taccgttttc cggctgctcg cctgctcgcc tcccgactgc tcgccttctt
     5101 ctcgacttct cgactgctcg actacactcc cgccatggac atgaacctcc gccaccttgc
     5161 tggtggcgaa ttgactgatt ccctgccgga aacaccgcat gagctgccgt tcgatcgcgg
     5221 caagatcgac gaactgatcg accgggtgcg tcagggcgag ccggttgacc tgctgaccga
     5281 gatgctggcc tgcgtcgact ggcgcggctc gttcaccgag cccgacggcc gcccgatcac
     5341 gatcgaggac atcgcccggc tgatcgccta ctacaagcag aagttcgcgg atgtcggcgt
     5401 catctacatc gccgatctac tctcgaccga gttcatgacc gagcagcggg ctcggggcga
     5461 cgtcgtcttc tccgacgatc tcctccgcct cttccgggag caacccgagg tctggcagga
     5521 gatccgcctc ttcttccgcc gcaaggagtt cgtgaccgcg ctcctgaccc aatctcatgc
     5581 cagccggtgg gcctcgctga atccagacgc caggcggggg acgtcagacg ccagccagaa
     5641 gacgtcagac ttcagtggtc agatgtcaga aatggggacg gccgatcaag gctgagggac
     5701 catcgaccaa ctagcgtctg atgtctgatg tctgatgact acgcccgggc ggcggcttcc
     5761 ttttccatct tgtcgaagaa ctggccgacg attgtcttgg cggccggttg gatcatgcgc
     5821 gcgccgaccc tggccaacga gccgcgcacg ttggcgtcac ccttgtactt gacgatggtg
     5881 ccgccgttgt cctctaccag ctcgaggttg ccgacgccgg agacctggcc ctgcgggcca
     5941 ctcccctcga cggacatctt gtagctctcc ggctcgttct tgtcggagat cttgaccttg
     6001 ccggtgaagg tgccgcggat gaaggagatg ccgatcttca tcgtcgcgac atactcgtcc
     6061 ggcccgacct cttcgagctt ctcgcatccg ggcaggctcg ccttcagcac gtcgggatcg
     6121 agcaggaact gccagacctt ctcgcgcggc acgtcgaacc gatgctctcc cgacacttcc
     6181 atcgaaattt cccccggatt gaagccattt ccgcgctcgt gagatgaatc ggagcgcgtc
     6241 ctctcaggcc ggcagtctac cagagccaaa cgggcacgaa cgcgcctgga attcagcatc
     6301 acccggaacg gcatgctcca aacgggactt ctgacgcctt tgcgtcaggt gaccgtttcg
     6361 atagcctgca atgggaaccc cgtctgccgg tcgcgcgtca cctgcccggg gttacctcgt
     6421 ggctgacgcg gcgccgcccg ggcgcgttcg gtgagcactt cgcaatggtt tcctcgcgac
     6481 ccctcgcccc gaccagcacg tcgccacttc gctcgctccg ccgcgtttct gaccggcacg
     6541 attcgttcga cctcgacacc gatccagtct tcgaccccaa tgcccagcgg atcgcgaccg
     6601 cggacctcgg ccgaatcgcg ccagataccc gccgcagggg gtcgctcaag acatcaggag
     6661 tgctgccgcg ccacaccggt tatctgcacg gcgtttcgct ctcggccatc ctgcagatgc
     6721 tccacctcga gcggaagacc tgcgtggtcg aggtctcggc gcacggcctg ctgggctccc
     6781 tgatcctggt caacggcgag ctggtcgatg cctcggccgg cgatctttcg ggcgaagagg
     6841 ctgcctgcga aatcctgggc tggatcgatc ctcagaccgc gatcctcgcc ggagcggaaa
     6901 cgtttcggca caccgttcag cggccgatca cccaactgat catggacgcc gtccggatcg
     6961 tcgacgaatc cggcatgctc aacccggcca gcttgccgga gttcgaccga aaccccggcg
     7021 cgcgcgtgtc cggcgactgg caatggctga tcgactccct ctcgatggtc ggggcgacga
     7081 atgtccagat cgtgaccccg gattccctgc cgtccgagcg acgccagacc gggttcctcg
     7141 gcggcgaccc ggcggccgat ctcgcccgcg gcattcggac ctgggcgacc ctgcttggac
     7201 cggacgtgac cgaggtcgtc gcaacccgga gcgattgcat cgtcgtgctc gcggtgctcg
     7261 acgccgaccg ctcggagttc gtctatgcgg aggcgcccgg tctcgagacg gccgagctcg
     7321 tccgccgctc cctccgctca attcggaaat agtcgttagt agatggacgt tggtcgttgg
     7381 tagatggaag accatccaac gaccaacgtc catctaccat ccactgatct cccgtaccat
     7441 gacgcgagtc agttcagact catgtcgagg gagactccat ggcgaccaga acccggatca
     7501 acgcggcgca catcatcgcg acgcagaaca atgagcaccg tcacctgcgc gacggcgtgg
     7561 tggtctacga gggcgaccgc atcgtccacg tcggcaagtc gttcgacggc aacgcggacc
     7621 agacgatcga cctgccccac ggcgtcgtca cgcccggctt catcaatacg cacgcgcatc
     7681 tcgccgggtc gccgctcgac aagtcgttca tcgaggatgt cggcccgcgc cagttctacg
     7741 gctccggcct gttcgagtac ctgccggtcc gtggcggcgc gcaggatgcc gaagcggata
     7801 tcgcctgcct cgccttctcg atgaccgagc tgatgcgcac gggcacgacg acgatcatgg
     7861 agatcggtca tcgcgcgcct gaagcggtcg aggaagccga caaggtcggg atgcggctct
     7921 acatgggcct cggctaccgc tccggcaaat ggctgacgac cgacggcaag cgcgtctcgt
     7981 gggagtggga cgagccggcc gggtatgccg gaatggagcg ggcgatcaag ttcatcgagg
     8041 agtacgacgg caagtccaac ggccggatca agggcttcct ctcgccgatg cagatcgaca
     8101 cgtgcaccga ggatctgctg cggaagtcga aggatgcagc cgactcgatg ggcgtgccga
     8161 tcgcgctcca cgccgcgcag tcggtcaatg agttcaacga gatggtcgcc cggcacggcc
     8221 gcagtccgat cgagtggcta gaggacatca atttcctcga ctcgaacgtg atcctcggcc
     8281 acgccatcat cctggccgga gggacgtggg ccaactatgc cggcaacgac atcgagatcc
     8341 tggcccggac ccatgccaat gttgcccact gtgtctgggt cttcgcccgg cgcggaatcg
     8401 cgatggagtc attcgcgcgc tataccgagg ccggcgtcaa catgaccctc ggcaccgata
     8461 ccgcgccgca gtcgatgctc gaggcgctgc gctgggcagc ggtggtcagc aagttgatgg
     8521 accggcagac gggtgtcgcg acggcggccg atgtcttcaa ggcggccacg ctcaacggcg
     8581 cgaaggcact gaaccgggac gatctcggcc ggatcgaagt cggcgcgaag gcggatctac
     8641 tcatctggga cggcgatacg atcttcatgt cgccgctgcg tgatccgatc aagaacatcg
     8701 tctactcggc cgaggcggaa gatctgaaca cggtcatcat cgatggcgag atcaggatga
     8761 aggaccgcca ggtgctcggt gtcgacaccc gcaagcttgc cctcgatctc caggccgccg
     8821 gcgagcggat gtggccggag atggcccagt acgactggaa cggccgcgac gccgacaagc
     8881 tctcgccgca atcgttccct gagtggaagt gatcaacggt tgttggacgt tggacgttgg
     8941 acgttggacg ttggaatctt gtatctcatc catcgaccaa cgtccatcta cccgactccc
     9001 ggcagtgcgt gagcgcgcct cagaaaggaa gtcgatgcct gatctgggtc cagtggtcga
     9061 taacgtcaat gagcagcagt ttgagttgac cgttgacgga ctgaccgcat tcgccgccta
     9121 ccagcgtgag ggcgatgtca tcgtgttcac ccacactgag gtcccgacag agcatcaggg
     9181 caagggtgtc ggcggcgcgc tggctcgtgg agcgctcgat gccgcgcgcg ccgaaggcgc
     9241 tgaggtaatc cccctctgcc ccttcatcgc gtcctacatc cggaggcacc cggactacct
     9301 caacctcgtc agcgaacgaa accagcggcg actcaaattg caagcgagcc aatgatgtga
     9361 agcgacttga gcctggttat tcgtctcccg ggacgccgta cgtgggagcg agtgtgggat
     9421 tgatggcgcg gttgacgtag tcctgggcct gggggcgcca gagacgccag agcgcagcca
     9481 ggtgcgcgat cgggtcgtcc gaccagtcga cccgcagatc agtaatcggc cactcgacgt
     9541 cgccggcgat cagcatgccg gccgagtgga ccggtcccat ctcgcccccg caggccaccg
     9601 ctgcctggag cgctgcaatt agcctgtcgc ccagctcggc ctctggattc gcttcaaagg
     9661 cggcgatcat ctcgttcggg atgctctcgt cgcggagcaa gttgccggcc gcgaccgcat
     9721 cggtgccctg cgccgccgca tgggtgccga gcacgccgga gccgtt
//
LOCUS       DATAOZ010000384         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4892528, whole genome shotgun sequence.
ACCESSION   DATAOZ010000384 DATAOZ010000000
VERSION     DATAOZ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4892528"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VHR64_09790"
     CDS             complement(<1..215)
                     /locus_tag="VHR64_09790"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HEX3303162.1"
                     /translation="MFIQTEPTPNPASLKFLPGREVMTRGVLDFTDPDQTQSSPLARR
                     LFEVEGVSGVFLGTDFITVTKTEGEDW"
     gene            complement(377..1339)
                     /locus_tag="VHR64_09795"
     CDS             complement(377..1339)
                     /locus_tag="VHR64_09795"
                     /inference="COORDINATES: protein motif:HMM:NF021600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HEX3303163.1"
                     /translation="MIETTLSGSEPGRRGWSLRWLRIGGVALLIALALYFLGGALWLN
                     QIDDDPDFGLQSSVPHGGAKSVAVAADLIDREINTHRWVANDPWFMPGSLLDNMPNFQ
                     MGIIAALSRFGIELGDQIARTRGSSQIDPDLDTAAGLLKYPGTIWIFDPQTWFKPTAS
                     SESQYRRAMRALRNYNDRLAVNQAAFEIRADNLQATLDRIAADLGSASAALDHEVLTA
                     SGFWPDFAADDVFYANKGRLYAYFVLLRALGNDFDKVIRERDLGSAWTQMLDSFRSAA
                     LLQPWVVVNGPPDGQFLPSHLAAQGFFLLRARTQLREITNILQK"
     gene            complement(1685..>2529)
                     /locus_tag="VHR64_09800"
     CDS             complement(1685..>2529)
                     /locus_tag="VHR64_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303164.1"
                     /translation="AVLCINRVKSHTSFDRPVESGLTKLIAVGLGKQAGAQNVHRLGP
                     RGYTEVLPALAKIAIEHSPIAFGIALVENAHKQLCLVEGVEPDDFYATDERLLKLAKS
                     LIARLPFERLDGLVVEWIGKEISGSGMDPAVVGRTGIRSVPDPVRPFVNKLAVLGVTD
                     DSYGNAVGLGNADYTTRKVANHIDLMPMYMNSITAAGTEGARIPAVLKDDRAVLQAVV
                     ATCWRADFENARLCQIRSTLYLSEILVSPGLLPDLEGRPDAKRLSDPAPLQFSAEGEL
                     LTRV"
BASE COUNT          443 a          831 c          833 g          422 t
ORIGIN      
        1 taccagtcct ccccctcggt cttggtcacg gtgatgaagt cggtgcccag aaagaccccg
       61 ctgacgcctt cgacctcgaa cagccggcgc gccagcggcg agctctgggt ctggtccgga
      121 tcggtaaagt cgagcacgcc ccgggtcatc acttcgcgcc ctggtagaaa tttgaggcta
      181 gcgggattcg gggtcggctc ggtctggatg aacatcatgc gcctcctagt tgcgccgggc
      241 ggggcggagc gatccgcggg ctatcggtcc ggcaccccgg gcgcttcggc atcggcgatc
      301 tcccaagatg gcccaagccg taccgccgtt caagccggac gactgccgaa agtcacctcc
      361 gcccgccccc gagcccctac ttttgcagaa tattggtgat ctccctcagc tgcgtgcgtg
      421 cgcgcagcaa aaaaaagccc tgtgctgcca aatggctggg caggaactgg ccgtcaggcg
      481 gcccgtttac gacgacccac ggttggagca acgcggcgct ccgaaagctg tcgagcatct
      541 gcgtccaggc agaacccagg tcgcgctccc ggatcacctt gtcgaagtcg ttgccgagcg
      601 cccgcagcag gacgaagtag gcatagagcc ggcccttgtt ggcatagaac acgtcatcgg
      661 ccgcaaaatc cggccagaag ccggaagccg tgagcacctc gtgatcgagc gccgcggatg
      721 ccgagccgag atcggcggcg atccggtcca gggtcgcctg gagattgtcc gcacggatct
      781 cgaaagccgc ttgattgacc gcgagtcggt cgttgtagtt gcgcagcgca cgcatggccc
      841 gccggtactg agattccgag ctcgcggtcg gcttgaacca ggtctgcggg tcgaagatcc
      901 agatcgtgcc ggggtacttg agaagaccgg ccgcggtatc gaggtccggg tcgatctggc
      961 tcgagcctcg ggtgcgcgcg atctggtcgc cgagctcgat cccaaagcgg gacagcgcgg
     1021 caatgatgcc catctgaaag ttgggcatgt tgtcgagcag gcttcccggc atgaaccacg
     1081 gatcattggc tacccagcga tgagtgttga tctcgcgatc gatcagatcg gcggccacgg
     1141 cgacgctctt cgcgccacca tggggcaccg agctctgaag cccgaagtcc ggatcatcgt
     1201 cgatctgatt cagccagagc gcgccaccca ggaagtagag cgccagcgcg atcagcaatg
     1261 cgacgccgcc gatcctgagc cagcgcaggc tccagccgcg cctgccgggc tcgctgccgc
     1321 tcagcgtcgt ctcgatcatg tcgactccgg ttggcgagaa gcgggattca ggcaccatgt
     1381 tggtgcttgc gccactcagt caatcgcgat gccctccggg atcgcgatcg aactccatga
     1441 cctcagccgg atcggcttgg cgccggaccc cggcgagcgc tctgcctgac agatcgagcc
     1501 ccgtccttgc agatgcagtc gtgcggctcg cggcagcgcc ccgcgcatcc gggtcggcct
     1561 cgaggcgggt acatcgaaca gtgagcgcaa ccgacgccga ctggggcctg gctcgcccac
     1621 gtcagctgaa gctgctcaca tcatgcgaac ctcagagccc gtcatgagcg gagaacatgc
     1681 tggttcacac ccgcgtcagc agctcgccct cggcggagaa ctggaggggt gccggatcgg
     1741 aaaggcgctt ggcgtcaggc cggccttcga gatcgggcaa caggcccggg gagaccagga
     1801 tctcgctgag ataaagcgtg gagcggatct ggcagagccg ggcgttctcg aagtcggcgc
     1861 gccagcaggt cgcgacaacc gcctgcagca ccgcgcggtc gtccttaagc accgccggaa
     1921 tgcgggcgcc ttccgtaccc gcggcggtga tcgagttcat gtacatgggc ataaggtcga
     1981 tgtggttcgc gaccttgcgc gtggtgtagt cggcgttgcc gaggcccacc gcattgccgt
     2041 aggagtcgtc ggtcacgccc agcaccgcga gcttgttgac gaacggccgg acgggatcgg
     2101 ggacgctgcg gatgccggtg cggccgacca ccgccgggtc catgcctgag ccgctgatct
     2161 ccttgccgat ccactcgacc acgaggccgt cgagtcgctc gaacggcagc cgcgcgatca
     2221 gcgacttcgc gagcttcaac agacgctcgt cggtcgcata gaaatcgtcc ggctccacgc
     2281 cctcgaccag gcaaagctgc ttgtgcgcgt tctcgaccag cgcgatgccg aaggcgatcg
     2341 gcgagtgctc gatcgcgatc tttgcgagcg ccggcagcac ctcggtgtag ccgcgcgggc
     2401 cgagccggtg gacgttctga gcgccggcct gcttgcccaa cccgaccgca atgagcttgg
     2461 tgagcccgct ctcgaccggc cggtcaaagc tcgtgtgcga cttgacccgg ttgatgcaca
     2521 gcaccgcgt
//
LOCUS       DATAOZ010000385         5216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6734023, whole genome shotgun sequence.
ACCESSION   DATAOZ010000385 DATAOZ010000000
VERSION     DATAOZ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6734023"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VHR64_09805"
     CDS             complement(<1..578)
                     /locus_tag="VHR64_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303165.1"
                     /translation="MDTTPFPDRTLTSETLENDVTAHQVLAWAPFVITTIAIAITYSR
                     LPVSSFYHVSVDGLAGGLGRALVHLNFPVSIAAIALIGIAVLRMRRSGLVATPPARRR
                     LTIIAGLGTLLCLVTGVPGVVDNGDLDARPINAFPAVGVLIAVALTVASFRAPDRYQR
                     MPWTTRDRIGVGLVATLFLFALPWMLADWGVYV"
     gene            complement(789..3107)
                     /locus_tag="VHR64_09810"
     CDS             complement(789..3107)
                     /locus_tag="VHR64_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3303166.1"
                     /translation="MTTALSDARSDLPIPPTSLIGRDRERSAIRVALAQEGVRLVTLT
                     GPGGIGKTRLALDAATHLDPAIADQVTLVPLAAVRDSELVLPTIAQAIGVREGAGRPT
                     RAAIEEVLRDRRLLLILDNLEHLLDAVPDIAWLLATSPGIRILATSRSRLQIQGEHEF
                     AVLPLPLPEPDQVADFANPAVALFVARARAIDPAFEPTVANLPVIAEICGRLDGIPLA
                     IELAAARVKVLPPDALLNRLDRRLPLLTGGARDLPDRLRTMRDAIAWSYGLLDADQQS
                     RFRRLAAFTGGFTLHEAQALIGRPDNEDSLLDALAGLVDASLLRQSEREGEPRFVMLE
                     TVREFGLEQLHECGEYDQVQSRHADIFLTFAEAATTRLRGAERTTWLERMERAHNNLR
                     AALAWCVERRDTRRALRLAGALWQFWWWRAHLAEGRQWLERALALPDAGAWPTPRARV
                     LTGLGALAETQGDYTAAEQYHDAAERAWHESGDSRGLAISLLFRWLVAFNADDQERMT
                     TLSTESLHLFRELDDPWGIAMSLMEQGVMAMRRQDHADAERILSEGIERFRAINDQWG
                     VAICEGVLGNVATDQGDYARAASLLASSLSSLLLLNDLWGVATVLPAASRMAGEQGRF
                     EWATRISGAIARMHETMGAPLKVPFRIRYEQNLAAARAALGDEQFNAAFAAGHAMTPE
                     QAVAEALHAPDTGETAPTPPSHPAMPIPLSPREREVLRLVPERTAREIGEELFISEST
                     VRTHIENILNKLGLRNQKELIAYAYQHGLIQS"
     gene            3383..4075
                     /locus_tag="VHR64_09815"
     CDS             3383..4075
                     /locus_tag="VHR64_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303167.1"
                     /translation="MTEHEAVFGGHVPRGDERLGIVDWIKFGLREADTGDELAHGQIV
                     CVAARWILVGAGLVLALWNPAALGPLRLQVLVLLMIAVANFYLHAQILRKRAAFAGVA
                     YAASAADLAVITTLVLSQGGFDSNLWVFYFPAVLALSVAFPPALTALFTGATIGIYGL
                     ICLSDTSAWNSSDGVQVVFVRLLMIASVAVCGAIYRQVEAGRRASVAIGEPDLLPGLR
                     PTSISDRTTEAN"
     gene            4116..4700
                     /locus_tag="VHR64_09820"
     CDS             4116..4700
                     /locus_tag="VHR64_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303168.1"
                     /translation="MRLLNETARDAAEDIYFGQVVLIWARWFVILAAAVLALWSSETT
                     GDLATRSLLVVALMGVNFFLHGRTLMEKPANRQLLLLSSAIDLAIVGGIVAAWGVAGL
                     ESELYVLYYPLLFAFALVFVPRYTIIYAFAALGSYAAICLMADPGLLTNTGDAKTLAQ
                     RLITMGATAGLGAFYWRIQRARRAAATVVALPLS"
     gene            4731..>5216
                     /locus_tag="VHR64_09825"
     CDS             4731..>5216
                     /locus_tag="VHR64_09825"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX3303169.1"
                     /translation="MAGGRFGKLLKSSVGSLLTPAEDPRATYADPVQRQRALREQVRA
                     ATASAQAARTRLQLQRATAEAGLSELEQAARSALAAGREDLARIALKRRHVSTMSIAQ
                     LAQQIAAMDAELHRLAVVDQQVGAQLDAIAAREHLAAVRQSTAQAQIEIGEVLTGVTG
                     VA"
BASE COUNT          858 a         1663 c         1850 g          845 t
ORIGIN      
        1 acgtagacgc cccagtcggc cagcatccag ggcagggcga acaggaagag ggtcgcgacg
       61 aggccgacgc cgatccggtc gcgcgtggtc cacggcatgc gctggtagcg gtcgggcgcg
      121 cggaaggaag cgacggtcag agcgacggcg atcaggacac cgacggccgg gaaggcgtta
      181 atcggccggg cgtcgagatc gccgttgtcg accacacccg ggacgcccgt gaccaggcag
      241 agcagcgtgc cgagtccggc gatgatcgtc aaccgccgcc tggccggtgg cgttgccacc
      301 aagcccgagc gccgcatccg caggaccgcg atgccgatca gcgcgattgc cgcgatcgag
      361 accgggaagt tcaggtggac gagcgcgcgg ccgaggccac ccgccaatcc atccacgctc
      421 acgtggtaga agctcgacac aggcaaccgg gagtaggtga tcgcaatcgc gatcgtggtg
      481 atcacgaatg gcgcccaggc caggacctgg tgcgcggtga catcgttctc gagggtctcg
      541 gaagtcaggg ttcgatccgg gaatggagtt gtgtccatcg tggtgcctgc ctatgcgtga
      601 aggagtccgt cctgaccgag catcatgcgg cgatcgccag cgaacatccc cggaactggg
      661 gattcgccgt cgattccgtt atccgccccc gaggttccag gcgcgaccat tcaaacgcgg
      721 ccgacgttgc aaattcgcct cagtgcatca gtgtgcggga ggcgcccccc ctttatccgg
      781 ctcacgcctc acgactgaat cagcccatgc tggtaggcgt aggcgatcaa ctccttctgg
      841 ttgcggagac cgagcttgtt gaggatgttc tcgatgtgcg tgcggaccgt gctctcgctg
      901 atgaagagct cctcgccgat ctcgcgggcg gttcgctccg gcaccagacg cagcacctcc
      961 cgttcgcgcg gcgaaagcgg gataggcatg gccggatggg acgggggcgt gggcgcggtc
     1021 tcgccagtgt ccggcgcgtg taacgcctca gcgaccgcct gctccggggt catcgcatgg
     1081 ccggcggcga aggcggcgtt gaattgctcg tcgccgagcg cggcccgggc ggcagccagg
     1141 ttctgctcgt agcggatccg gaacgggacc ttgagcggcg cgcccatcgt ctcgtgcatc
     1201 cgggcgatcg cgcccgagat gcgggtcgcc cattcgaacc ggccctgctc gccggccatg
     1261 cgcgaggcag ccggcaggac cgtggccaca ccccagagat cgttgagcag gagcaggctg
     1321 ctcaggctcg acgcgagcag ggaggcagcc cgcgcatagt caccctggtc ggtcgcgacg
     1381 ttaccgagga caccctcgca gatcgcgacg ccccactggt cgttgatggc gcggaagcgc
     1441 tcgatgccct cggacaggat ccgctcggcg tctgcgtggt cttgccggcg catcgccatc
     1501 acgccctgct ccatcagcga catggcgatg ccccacgggt cgtcgagctc gcggaagagg
     1561 tgcaggctct cggtcgagag ggtggtcatc cgctcctggt cgtcggcgtt gaaggcgacg
     1621 agccagcgga agagcagcga gattgccagg ccgcggctat ccccgctctc gtgccaggcg
     1681 cgctcggccg catcgtgata ttgctcagcg gcggtgtaat cgccctgggt ctcggcgagg
     1741 gcgccgagac cagtcaagac ccgggcccgg ggagttggcc aggcaccggc atcggggagg
     1801 gccaatgcgc gttccagcca ttgccggccc tcggccagat gcgcgcgcca ccaccagaac
     1861 tgccagagcg ccccggccag acgcagggcc cggcgggtgt cacgccgctc gacgcaccag
     1921 gcgagggcgg cgcggaggtt gttgtgggcg cgctccatcc gctccagcca ggtcgtgcgc
     1981 tcggctcccc gcagacgagt cgtggcggct tcggcgaagg tcaggaagat gtccgcgtgc
     2041 ctgctctgga cctggtcata ttcgccgcat tcgtgaagtt gctcaaggcc gaactcgcgg
     2101 accgtctcaa gcatgacgaa gcgcggctcg ccctcgcgct cggattggcg aagcagactg
     2161 gcatcgacca ggcccgccag cgcatcgagc aggctgtcct cattgtccgg ccggccgatc
     2221 agtgcttggg cttcgtggag cgtgaagccg ccggtgaagg cggcaagccg gcggaagcgg
     2281 gattgctgat cggcgtcgag caggccgtag ctccaggcga tggcatcacg catggtgcgg
     2341 aggcgatcgg gtaaatcacg cgcgccgccg gtcaacaggg gcaggcggcg atcgaggcgg
     2401 ttgagcagag catcgggcgg caggaccttg acccgcgccg ccgccaactc gatcgcgagc
     2461 gggatgccgt cgagccggcc gcaaatctcg gcaatgaccg gcaggttcgc aactgtcggc
     2521 tcgaaagcgg gatcgatcgc gcgtgcccgc gctacgaaga gcgcgacggc cgggttggcg
     2581 aagtcggcga cctgatcagg ctcaggcagc ggcagcggga ggacggcgaa ctcgtgctcg
     2641 ccctggatct ggagacggga ccggctggtg gccaggatcc ggatgccggg actggtcgcg
     2701 agcagccagg cgatgtcagg aaccgcgtcg agcagatgct cgaggttgtc gaggatcagg
     2761 agcaggcggc gatcgcgcag cacctcctcg atcgcggcgc gggtcggccg tccggcgcct
     2821 tcgcgcacgc cgatcgcctg ggcaatggtc ggcagcacaa gctcagaatc gcgcacggcg
     2881 gccagcggga cgagcgtgac ctgatcggcg atggctggat cgaggtgggt ggccgcatcg
     2941 agggcgagcc gggtcttgcc gatgccgccg ggaccggtca gcgtgaccag gcggactccc
     3001 tcctgagcca gggccacgcg gatggcagac cgctcgcgat cgcgaccgat cagggatgtg
     3061 ggtggaatcg ggagatcgga ccgggcgtcg gagagcgctg tcgtcatgaa ggtccttatc
     3121 cgaatgtcat gtatccgtga tcttagccga gttcagggga caggggacag tcgacggcgg
     3181 acagtcgacg ggggacagtg ggcgagaacg ctctgtccaa aacccaaaac ccaaaaccca
     3241 aaaccgagcg cttgtcccct gaactgtgga tgccccgccc gctgtcggtt tccgcagaaa
     3301 tcgggatgtc ctctcgcact ggcccgggca tggtggaggc gtgaggcgat cggggaatcg
     3361 ccggacgcgg aggaggacca ccatgacgga gcacgaggcg gtatttggcg ggcacgttcc
     3421 acgcggcgac gagcgactgg gcattgtcga ctggatcaaa ttcggcttgc gggaggcaga
     3481 caccggagac gagcttgccc acggccagat cgtctgcgtg gcggcccgct ggatcctcgt
     3541 cggggctggt ctcgtgctgg cgctttggaa cccggcggcg ctgggaccgc tgcggctgca
     3601 ggtgctggtg ctgctgatga tcgcggtcgc caacttctat ctgcacgcgc aaatccttcg
     3661 gaagcgagcg gcctttgccg gcgttgccta tgccgccagc gcggccgacc tggccgtgat
     3721 cacgaccctg gtcctgagcc agggcggatt cgattccaat ctctgggtct tctacttccc
     3781 ggcggtgctg gccctctcgg tcgcattccc gccggccctg actgcactgt tcaccggcgc
     3841 gaccatcggg atctacgggc tgatttgcct gagcgacacc agcgcctgga actcaagcga
     3901 cggcgttcaa gtcgtcttcg tccgcctgct gatgatcgcc tcggtcgcgg tctgcggggc
     3961 gatttaccgg caggtcgaag ccgggcgccg cgcgtcggtc gcgatcggag aacccgatct
     4021 gctgcctggc ctgcgcccga cctccatatc cgaccgaacg acggaggcca actgagcgcg
     4081 agtcgctcgc gcatcaggat gaagggagat tcgccatgcg tctactgaat gagaccgccc
     4141 gggatgcggc ggaagacatc tacttcggac aagtcgtgct gatctgggcg cgctggtttg
     4201 tgatcctggc cgccgcggtg ctggcgcttt ggtcatcgga aacgactggc gatctcgcga
     4261 cgcggagcct gctggtcgtg gcgctgatgg gcgtcaactt cttcctgcat gggcggacac
     4321 tgatggagaa gccggccaac cggcaactcc tgctcctgag cagcgcaatc gatctggcaa
     4381 ttgttggcgg gatcgtggcg gcatggggcg tggccggtct ggagagcgag ctctatgttc
     4441 tctattaccc gctgctcttc gctttcgcgc tcgtcttcgt tccgcggtac acgatcatct
     4501 atgcatttgc ggcgctcggc tcgtacgcgg cgatctgctt gatggcagat ccagggctgc
     4561 tcaccaacac gggcgatgcc aagacccttg cgcagcggct gatcacgatg ggcgcgactg
     4621 ccggcctggg cgccttctac tggcgaatcc agcgggcccg gcgcgctgcc gcgacggtcg
     4681 ttgcgttgcc tctctcgtag ctgtcagatg acgacgaaag gggtagcgag atggcaggcg
     4741 ggcgattcgg gaagctgctc aagtcatcgg tcggctctct gctgacgccg gccgaggacc
     4801 cccgagcaac ctatgccgat cccgtccagc ggcaacgggc gctccgcgaa caggtacggg
     4861 cggctacggc cagtgcccag gcggcccgta cccggcttca gcttcagcgc gcaaccgccg
     4921 aagcgggcct ctccgagctg gaacaggcgg cccgctcggc tctcgcagcg ggccgggagg
     4981 atctggcccg gatcgcgctc aagcggagac acgtctccac catgagcatc gcccagctcg
     5041 cgcagcagat agcggccatg gatgccgagc tgcaccggct ggccgtggtt gatcagcagg
     5101 tcggtgccca gctcgacgcg attgccgctc gcgagcattt ggcggcggtg cggcaatcga
     5161 ccgctcaggc gcagatcgag atcggcgagg tgctcactgg cgtgactggt gtagct
//
LOCUS       DATAOZ010000386         6397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4101818, whole genome shotgun sequence.
ACCESSION   DATAOZ010000386 DATAOZ010000000
VERSION     DATAOZ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4101818"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1168
                     /locus_tag="VHR64_09830"
     CDS             134..1168
                     /locus_tag="VHR64_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303170.1"
                     /translation="MMANELDRKAAELSMAWDLLLAGDQVDRAATELELIQTVRQISD
                     IDSGASISADIEKMIWTNVIAGTSERWRPEIPTAGVETAEARPALRANVVSLWIVRLA
                     WMVAAGFAGGFFAGIISRLTMRLAGFLTVDQNRFMLTDNGNRVGKITVEGTLFLGTLT
                     GALGIVTILIYALLRNRLPFTGWQRSLGYGVMLLAVFGYVLMDPANEDYHLFGPAWLN
                     VATFSSLYIFLGFFTSQFHEWGMRRNLPARALAFRPVVRFPVLGMATVLAGIGFLVSV
                     SAMIVGAPGMIVIGLGAVAWLINRFALKDRLASLSAPRLVQTWGMLVVPGIIGFILTA
                     RGITEILLNR"
     gene            1197..2003
                     /locus_tag="VHR64_09835"
     CDS             1197..2003
                     /locus_tag="VHR64_09835"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX3303171.1"
                     /translation="MSGAEKEVVIMTVPSTRRAFLGFAAGTIGALVASRVPAIAAPVR
                     PATSARTLGQETLAGPFTLPLLDYATHALEPHIDQTTMEIHHGKHHQAYIDNLNKAVA
                     EHPEIAELPVWDVLTDLTIVPEAVRPTVRNNLGGHVNHTIYWELMTPKSLDPSQRLVD
                     AITRDFGSVEEMQAAVNEAGLKRFGSGWAWVVSNGGALSVVSTPNQDSPVMDGAGFPI
                     IGIDVWEHAYYLKYQNKRADYLAAWWNVINWRTVDELYAWSMEAEATPTA"
     gene            complement(2006..2800)
                     /locus_tag="VHR64_09840"
     CDS             complement(2006..2800)
                     /locus_tag="VHR64_09840"
                     /inference="COORDINATES: protein motif:HMM:NF018288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HEX3303172.1"
                     /translation="MIDLSSRVGMPIALDPESGTLAFGDGVAVEGTGERRFAELRDVL
                     ADPDALDDERAEQPAYLLYRGVHCPSDARLLADHGLRYDLTVTLPGEVGGELVKTAGH
                     IHNAAPDGVGYPEIYDVIHGHAAFILQEDEPLRVSIITCDPGERILIPPGASHLTVNI
                     GRQPLVVADLVAIASQNDYGEFRTRRGAAVHLFRDGDAWSERINRAYPTTPVWRVLNG
                     TRIGDFAPGDAPIYTDAIANPGDYHYLTAPAPREAEMQALWSSRDR"
     gene            complement(2804..3358)
                     /gene="hxlB"
                     /locus_tag="VHR64_09845"
     CDS             complement(2804..3358)
                     /gene="hxlB"
                     /locus_tag="VHR64_09845"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR03127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HEX3303173.1"
                     /translation="MSDQITDLARQICDEIATAVGSVPDTDRRRAIELVTAARRIFVF
                     GEGRSGLVLRMAAMRLVHLGLTANVVGDATTPAIGAGDLLIAASGSGETAVTLLIAEQ
                     ARRAGATLLAVTANVAGSLAASADATLLLRTPAKGDRGAGQSIQPGGSLFEQSMLVLF
                     DTLFLMIAGDTAAQQIEERHANLE"
     gene            complement(3355..3999)
                     /locus_tag="VHR64_09850"
     CDS             complement(3355..3999)
                     /locus_tag="VHR64_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX3303174.1"
                     /translation="MSHFRFSGAIFDLDGTLVQSEHLHRQSWIEPLEELGITVDEDAY
                     LRDFAGKPGMQIIRDHVGLEGDAAVSLYERVTNSYWQLAVEHARPTPGVVDFLDLIAA
                     TPKSVCTSAERDSALRMLDMLGLTWRFDAIVTATDVTQGKPDPEPFLLAASRIGIDPA
                     RCVAFEDSTNGLISARAAGMTCIGIGPGFGASAQLADGWIVDFTDPVLNQLVME"
     gene            complement(3996..4640)
                     /gene="hxlA"
                     /locus_tag="VHR64_09855"
     CDS             complement(3996..4640)
                     /gene="hxlA"
                     /locus_tag="VHR64_09855"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: protein motif:HMM:TIGR03128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043801 - hexulose-6-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019647 - formaldehyde assimilation via
                     ribulose monophosphate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HEX3303175.1"
                     /translation="MTKLQLAIDLVPLDELERVILPLLSHVDVVEAGTPLIKRHGLDA
                     VRTLRTLALDHLLVADMKTMDAGALEAEMAFRAGADLMTVLACASDATVSAAVDVARQ
                     QGTSVVADLIGVPDKPARARQLVELGIEWLGIHTGTDDQAAGANPLADLQSVRDAVDA
                     RLVVAGGINQRTLPSILACSPAIVIVGSGILGQPEPVAAAASLRAILDQSRAGS"
     gene            complement(4637..5827)
                     /locus_tag="VHR64_09860"
     CDS             complement(4637..5827)
                     /locus_tag="VHR64_09860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303176.1"
                     /translation="MVDARPYRRDTITLFLLATTGMHRSVFHSMGAVMPALRKDLDIS
                     ATLTNAHVSAIALGMMAMGLMADRVAGRIGRRPAVWLGMIGIVLGMVLLALAPVVWMT
                     LLIALLFIGALGSLVLALTPAALSDHQGENRAVALAESSLVSSVIGGIVPAVVGLSQR
                     LGFGWGGASIVPVGLLGLLAAKFRHTPIPDPGSHPDHPGRSEPLNRGFWSFWLTLVFT
                     DSFEFGLIFGAAVLLEYRGMSQAGAATALSLLFWGMIVGRFAGSRLVRRISPIRLLRI
                     TFALAGIGIVAHWLSPVLALSLVGLFLAGIGISNLYPLTTSIGLQAAEFNSSAAGSRL
                     SLASGISMLVAPLIFGVLTDLADIRVAFAVEPLFLLLAIASAGYGARWLARPNVNAAE
                     AYAI"
     gene            complement(5838..>6397)
                     /locus_tag="VHR64_09865"
     CDS             complement(5838..>6397)
                     /locus_tag="VHR64_09865"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX3303177.1"
                     /translation="PTIYYGEELGMENVEIPPHMEQDPAGLRVPGIGLGRDPERTPMQ
                     WDSSPNAGFAPEGVMTWLPLAPDYETRNVAVEKADPKSILNLTNELLELRKSHPALAV
                     GDYAAIDGVPDECYVYTRSTSGQRMLVALNLTNQEHQFTLPYTGTGQVVTSTGKDRTG
                     KISLTFMVLQPNEGILIDITGAKLA"
BASE COUNT         1149 a         1998 c         2203 g         1047 t
ORIGIN      
        1 ccggactgag cgcgaacgag atcgcgaccg cgctcggcaa gtcccgaaat gcgatcgatc
       61 agatgcaata tcgcgcggtg gctcgtctcc ggaaattgct gacagctccg gtcctgcaga
      121 tggaagaggc acgatgatgg cgaacgagct cgatcgcaag gccgcagagc tgagcatggc
      181 ctgggacttg ctgctggccg gcgaccaggt cgaccgggct gcgacagagc ttgagctgat
      241 ccagaccgtg cgccagatat cggatatcga ttccggtgca tcgatcagcg cggacatcga
      301 gaagatgatc tggacgaacg tcatcgccgg gacctccgag cggtggcggc cggagatccc
      361 gaccgcgggg gtggagacgg ccgaggcgcg gccggcgctg cgtgccaatg tggtctcgct
      421 ctggatcgtg cggttggcct ggatggtcgc ggcaggattt gcgggcgggt tcttcgcagg
      481 gatcatctcc cggttgacga tgcgcctggc cggatttctg accgtcgatc aaaaccggtt
      541 catgttgact gacaacggca accgggtcgg gaagatcacg gtggagggga cgctcttcct
      601 cgggacgctc acgggcgcgc tcggcatcgt cacgatcctg atctacgcgc tgctgcgcaa
      661 ccggcttccc ttcaccgggt ggcagcggtc gctcgggtat ggcgtgatgc tgctggcggt
      721 cttcggttac gtgctgatgg acccggccaa cgaggattac cacctgtttg gaccggcctg
      781 gctcaatgtc gccacattca gctcgctcta catcttcctg ggcttcttca cgagccagtt
      841 ccacgagtgg ggcatgcggc gcaatctgcc ggccagggcg ctcgctttcc gaccagtcgt
      901 gcgcttcccg gtgttgggta tggcgacggt gctggcgggc atcggtttcc tggtctccgt
      961 ctccgcgatg atcgtcggcg cgcccgggat gatcgtgatc gggctcggcg ccgttgcctg
     1021 gctgatcaac cggttcgcgc tcaaggaccg cctggcgtcg ctttcggcgc cccgacttgt
     1081 tcagacctgg ggcatgttgg tcgttcccgg aatcatcgga ttcatcctga ccgcgcgggg
     1141 gatcaccgag attctgttga atcgataaat cgtccgttca attgagacgc gccgcgatga
     1201 gcggcgcaga gaaagaggtt gtcatcatga ccgtaccttc gacacgtcgc gccttccttg
     1261 gcttcgctgc tggaacgatt ggggcgctgg ttgccagccg cgtgccggcc attgcggcgc
     1321 cggtccgccc ggcgacgagc gcgcgaacgc ttgggcagga gacgctggcc ggcccgttca
     1381 cgctcccgct gctcgactat gcgacccatg cgctcgagcc gcacatcgat cagacgacga
     1441 tggagatcca ccacggcaag caccaccagg cctacatcga caacttgaac aaggccgtcg
     1501 ctgaacaccc ggagattgcc gagctcccgg tctgggacgt cctgaccgac ctgacgatcg
     1561 ttcccgaggc ggtacgcccg acggtgcgca ataaccttgg cgggcacgtc aaccacacga
     1621 tctactggga gctgatgacg ccgaagagcc tcgatccgtc gcagcgcctg gtggatgcga
     1681 tcacgcgcga cttcggttcg gtcgaggaga tgcaggcagc cgtcaacgag gcgggactga
     1741 agcgtttcgg atcgggttgg gcctgggtcg tcagcaacgg cggcgccctg agcgtggtct
     1801 cgacgccgaa ccaggacagc ccggtcatgg acggcgccgg attcccgatc atcggcatcg
     1861 acgtctggga gcatgcctac tacctcaagt accagaacaa gcgggctgat tacctggcgg
     1921 cgtggtggaa cgtgatcaac tggcggaccg ttgacgagct ctacgcctgg tcaatggagg
     1981 cggaggcgac cccaacggcg taggttcagc gatcgcgact tgaccagagc gcctgcatct
     2041 cggcctcccg gggtgcgggc gcggtcaggt agtggtaatc gcccgggttc gcgatcgcgt
     2101 cggtgtagat cggggcgtcg cccggcgcga agtctccgat acgggtgccg ttgagaactc
     2161 gccagaccgg cgtggtcggg taggcccggt tgattcgctc ggaccaggcg tcgccgtcgc
     2221 ggaagaggtg gactgccgcg ccgcgacggg tgcggaactc gccgtagtcg ttctgggagg
     2281 cgatcgcgac gagatcggcc acgacaagag gctgccggcc gatgttgacg gtgagatggc
     2341 tcgccccggg cgggattaat atccgctcgc cgggatcaca ggtgatgatg gacacccgta
     2401 acggctcgtc ctcctggagg atgaacgccg catgaccatg gatgacgtcg taaatctcgg
     2461 ggtagccgac cccatccggc gccgcgttgt ggatgtgacc ggccgtcttg accaactcgc
     2521 cgccgacttc gcccgggagc gtgacggtca ggtcatatcg gaggccatgg tcggccagca
     2581 ggcgcgcgtc cgatgggcaa tggaccccgc gatagagcag ataggcaggc tgctcggcac
     2641 gctcgtcatc cagcgcgtcg ggatcggcca ggacgtcgcg cagctccgcg aatcgacgct
     2701 cgcccgtgcc ttcgacggcg accccatcgc cgaatgccag cgtgcccgac tccggatcaa
     2761 gggcgatcgg catgccgacc cgggaagaaa gatcaatcac gagctactcc aggtttgcgt
     2821 ggcgctcttc gatctgttgc gcggcggtat cgcccgcgat catcaggaag agcgtatcga
     2881 agagtaccaa catgctctgc tcgaagagcg aaccgccggg ttggatcgat tggccggcgc
     2941 cgcgatcgcc cttggccggc gttcggagca agagcgtggc atcggccgag gctgccagtg
     3001 agccggcgac attggcggtc accgcgagca gcgtcgcccc agcccggcga gcctgctcgg
     3061 cgatcagcag cgtgacggcg gtctcgccgg aaccggaggc tgcgatcagg agatcgcctg
     3121 caccgattgc gggcgtggtg gcatcgccga cgacgttcgc ggtgaggccg agatggacga
     3181 gccgcatcgc cgccatgcgc aggaccaggc cggagcgtcc ctcgccgaag acgaagatgc
     3241 ggcgggccgc ggtgaccagc tcgatcgcgc gccgtcgatc ggtgtcgggc acggaaccga
     3301 ccgccgtcgc gatctcgtcg cagatttgcc gggcgaggtc agttatctga tcgctcattc
     3361 catcaccagt tgattgagaa cgggatccgt gaagtcgaca atccagccgt cggccagttg
     3421 ggccgacgcg ccgaatcccg gcccgatgcc gatgcaggtc atgccggcgg ctcgagcgga
     3481 gatcaggccg ttggtcgagt cctcaaaggc gacgcagcgc gccgggtcga tgccgattcg
     3541 cgacgccgcc agcaggaagg gctccggatc tggtttgccc tgggtcacat cggtagcggt
     3601 cacgatcgcg tcgaagcgcc aggtcagacc gagcatgtcg agcatgcgca gcgcggagtc
     3661 gcgctcggct gatgtgcaga cggatttcgg tgtcgcggcg atcaggtcga ggaaatcgac
     3721 gacaccggga gtcggccgcg cgtgctcgac cgcaagttgc cagtagctgt tcgtgacccg
     3781 ttcgtaaagc gacacggcgg catcgccttc gaggccgacg tggtcgcgga tgatctgcat
     3841 ccccggtttg ccggcgaaat cgcggagata ggcgtcttcg tcgacggtga tgccgagttc
     3901 ctcgagcggc tcgatccagg attggcgatg caagtgctcg ctctgcacga gcgtgccgtc
     3961 caggtcgaag atggcgccgc tgaaccggaa atggctcacg acccggcccg ggattgatcg
     4021 aggattgcgc gtagtgacgc cgcggcggcg accggttctg gttggccgag aatgccggag
     4081 ccgacgatga cgatggccgg ggaacacgcc agaatcgatg gcaaggtgcg ctggttgatc
     4141 ccgccggcga ccacgagccg ggcatcgacc gcgtcgcgaa cggactggag atcggccagg
     4201 ggattcgcgc cggcggcttg atcgtccgtg ccggtatgga tgccgagcca ttcgataccg
     4261 agctcgacca actggcgcgc ccgcgcaggc ttgtcgggca cgccgatcaa gtcggcgacg
     4321 accgatgtgc cctgctgccg cgcgacatcg acggccgctg aaacggtggc atcgctggcg
     4381 caggcaagca cggtcatgag gtcggcaccg gctcggaagg ccatctccgc ttccaacgcg
     4441 ccggcatcca tcgtcttcat gtcggcgacc agcaggtgat ccagcgcgag tgtgcggagc
     4501 gtccgcacgg cgtcgagacc gtggcgcttg atcagcggcg tgccggcctc gaccacatcg
     4561 acgtgcgaca acagcgggag gattacccgc tccagttcat caagcggaac aagatcgatt
     4621 gcaagctgga gcttggtcat atcgcgtagg cctcggcggc gttgacattc ggacgggcga
     4681 gccagcgggc accatacccg gccgaagcaa tggccagcaa caggaacaac ggctcgacgg
     4741 cgaaggcaac ccggatatcg gccagatcgg tcaggacgcc gaagatcaac ggcgcgacca
     4801 gcattgagat tccgctggcc agcgacaggc gggaaccagc cgcgctcgag ttgaattcgg
     4861 cggcctgcaa accgatcgat gtcgtcagcg ggtagaggtt cgatatgccg atccccgcca
     4921 ggaacagacc gacgagcgaa agcgccagca ccggacttag ccagtgggcg acgatgccga
     4981 tgccggccag cgcgaacgtg atgcggagaa gcctgatggg cgagatgcgc cggaccagcc
     5041 ggctgccggc gaaacgcccg acgatcatgc cccagaacag gagcgagagc gcggtcgccg
     5101 ctccggcctg cgacatcccg cggtattcga ggagcacggc cgcgccaaag atcaggccga
     5161 actcgaagga gtccgtgaag acgagcgtca accagaacga ccagaagcca cgattcaatg
     5221 gctcggatcg tccgggatga tcaggatgcg atccgggatc aggaatgggc gtgtggcgga
     5281 acttcgccgc gagcagaccg agcaagccga ccgggacgat cgacgccccg ccccagccga
     5341 atccgaggcg ctggctgagc ccgaccacgg ccgggacgat cccgccgatc acgcttgaga
     5401 cgagcgatga ctcggccagc gcaaccgccc ggttctcgcc ctggtgatcg gagagcgcgg
     5461 ccggtgttaa tgccagtacg agtgagccaa gcgcgccgat gaagagcagc gcgatgagca
     5521 acgtcatcca gaccacgggc gccaatgcca gcaggaccat gccgaggacg atcccaatca
     5581 tgcccagcca aaccgcaggg cggcggccga tccgcccggc cacccgatcg gccatcaggc
     5641 ccatcgccat catgccgagc gcgatcgcgc tgacatgcgc gttggtcagg gtggccgaga
     5701 tgtcgaggtc cttgcgcagg gcgggcatga ccgcgcccat cgagtggaac accgaccggt
     5761 gcatgccggt ggtggccaac aggaagagcg tgatcgtgtc ccggcgatag ggacgggcgt
     5821 cgaccatcgg ccgctatcta ggcgagtttg gcgccggtga tatcgatgag gatgccctcg
     5881 ttcggctgga ggaccatgaa cgtcaggctg atcttgccgg tccggtcctt cccggtcgag
     5941 gtcacgacct ggccggtgcc ggtataaggc agcgtgaact ggtgctcctg gttcgtgaga
     6001 ttgagcgcga cgagcatccg ctgtccggac gtgctccgcg tgtagacgta gcactcgtcc
     6061 ggcacgccgt cgatcgcggc atagtcaccg accgccaggg ccggatgcga tttgcgtagc
     6121 tcgagcagct cattggtcag gttcaggatc gatttcgggt ccgccttctc gaccgcgacg
     6181 ttgcgcgtct cgtagtccgg cgccaacggc aaccaggtca tgacgccctc cggcgcaaaa
     6241 ccggcgttcg gggacgagtc ccactgcatg ggcgtgcgct ccggatcgcg accgagaccg
     6301 atcccgggga cgcgcaggcc ggcggggtcc tgctccatgt gcggcggaat ctcgacgttc
     6361 tccatgccga gctcctcgcc ataatagatc gtcggcg
//
LOCUS       DATAOZ010000387         7457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8840374, whole genome shotgun sequence.
ACCESSION   DATAOZ010000387 DATAOZ010000000
VERSION     DATAOZ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8840374"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="VHR64_09870"
     CDS             complement(<1..2002)
                     /locus_tag="VHR64_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX3303178.1"
                     /translation="MATRAAGRPLRSGAKSPKPKSKQGADPRQSALSVAQGRISELGG
                     FLPSSSIFNRDLLGAVSALIGLITAWTVLAGESNGVLVEVVDRFLAWLCGRAPYVIPV
                     LTMLLSYQCFRNRQGRFIQIRQVVAGVAFGLSVTGLTQAAATGTRSERGGLIGGTTYD
                     YSRALVGDYAAAIGLFLIGSAAVLVATNSNPLLLYRELERIGRAIYKPLPPIEDTDVF
                     DTIPAVIEPPVEAAKPESVPASAPSLVINSPKKESIVKKGKDAVAKVVGGPKPSDEIL
                     ALPDIAQWHLYDQVTLDKGDLTAKARRIEETLTSFKVEARVREINPGPAVTQFTLEPG
                     LGTKVRRITELQNDLALALAAPSIRIEAPVPGLARVGLEIPNAKVATVGLRETLESER
                     FAHGKFKLPIPLGRDVNGKYVIHDLTKMPHLLIAGATGSGKSVCLTSIISTFLAFKRP
                     NELKMLMMDPKMVELIGYNGVPHLQCPVVTEMDKVVGALRLVLQEMERRYTLFSKMGV
                     RNLDGYRMKVADEAGAEEIPYLVVIIDELADLMMTAPDDVETVLCRLAQMGRAAGIHL
                     IIATQRPSVDVLTGLIKANVPARVAFAVTSLTDSRVVLDMPGAERLLGRGDMLFLPPD
                     AAKPVRIQGAFIEDGDVQYLVDHWHKVSPVPSYAPEWLELPSS"
     gene            2268..3599
                     /locus_tag="VHR64_09875"
     CDS             2268..3599
                     /locus_tag="VHR64_09875"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX3303179.1"
                     /translation="MTSLLLRNGRLIDPALGLDQTGDLLIVDGRIASVSSGPNATTAD
                     REVDCSGSIVSPGWIDMHVHLRVPGQEHKETIETGTAAAAAGGFTAIAVMPNTEPSLD
                     SVKTIHWLLDECRDRAVVRVLPIGAITIGRAGQELVDFDALADLGVVGFSDDGISTAN
                     SALMAEALAASKRIGIPIMVHCEDPYLVGGVMHEGEVSARLGLPGIMAAAEESFIARD
                     CLLAHETGGWLHVLHVSTGIGLQIVKQARALGAHVTVEVMPHHLVMTDHWVAGDRALQ
                     NTSERGPNAPAAHSDTKVNPPLRTADDTRALLAGLANGDFDILATDHAPHAAHEKRDV
                     PITKAAFGMIGLELAVPTMLALVRAGHLTMTQVIERFSTQPARLWHLLSGRIEPGGIA
                     ELTVIDPNHRWTVTEDALKSKSKNTPLLGMEMTGRAVMTISEGKVVHDLCK"
     gene            3583..4728
                     /gene="carA"
                     /locus_tag="VHR64_09880"
     CDS             3583..4728
                     /gene="carA"
                     /locus_tag="VHR64_09880"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX3303180.1"
                     /translation="MTSANDSSPKRYRPSHDAALALADGRVFRGRGFGAETDAGGEVV
                     FTTTMTGYQEVCTDPSFKGQLVCMTYPLIGNYGVAADDDESRQPWISALIVREHSDHP
                     SNWRSTGTIDEYLERCGIPGISGVDTRALTRHIRSVGDTRAVLVRNAADLTDEELVAR
                     AKHAPLPGEQPVVEEVSNDHVMTEGPDDGPHVVVLDCGVKRNIVRSLLNRGARVTSVP
                     YGTPFADIAALQPDGVIVSPGPGDPANLDDGLDVVRSLLDQKLPYFGICLGHQLLARA
                     IGADTVKLKFGHRGGNHPVLDTRSDHVSITAQNHGYVVKTDSLPNGSGWKVALVNLND
                     GSVEGFSHESLPVLSVQFHPEASPGPMDNGYLFDQFLDMVKERATAH"
     gene            4741..>7457
                     /gene="carB"
                     /locus_tag="VHR64_09885"
     CDS             4741..>7457
                     /gene="carB"
                     /locus_tag="VHR64_09885"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX3303181.1"
                     /translation="MSGAIKSVLVLGSGPIVIGQAAEFDYAGTQACTALREEGIRTIL
                     INSNPATIMTDEDVADAVYIEPLTPEVVRRVIQREKPDGLLPSLGGQTGLNLAVQVAE
                     QGILDRYGVRLLGTQLESIKAAEDRKLFKELLESIGEPAIQGQIVTSLEEAHAFAELV
                     PFPLVIRPAFTLGGTGGGIATNAQELDTIVTSGLGASPIHQVLLERSLQGWKEIEYEV
                     MRDSNDTCITICNMENIDPMGVHTGDSIVVAPSQTLSDKEYQMLRTSALKIIRALKIE
                     GGCNIQYTLNPDSFEYAVIEVNPRVSRSSALASKATGYPIARMAAKIAIGKRLDEMVN
                     QVTGKTTAAFEPALDYCVVKIPRWPFDKFAKADRSIGTQMKATGEVMAIDRSFEGALM
                     KAIRSLETNANDLGWEDKNWTSLDKLEALIRRPNDVRLWAVAAAMRRGVCADDIHAWS
                     KIDLWFLDKIAHLIDLERQIAAGPLTDDLLWESKRSGFSDDQIGRLAGTSAADVRARR
                     EAVGMNPVYKMVDTCAGEFASNTPYFYSTYEEEDEAIPTGGKSAVVVGSGPIRIGQGI
                     EFDYCSVQAAKALQDAGYDSIMINSNPETVSTDFDASDRLYFEPLDVESVHEVLKHEG
                     QVEAGMPPVIVQFGGQTAINLAEPLSERGVTIFGSSQDTIDIAEDRERFEAFLRGLGI
                     PQPHGAAVSTLIDATDVAERIGYPVLVRPSYVLGGRAMEVVYNEDHLARYIRNATNMN
                     PDHPVLVDKYLLGKEVEVDAICDGEEVLIPGVMEHIERAGVHSGDSFAVYPGIRLYPA
                     EIDTIVEYTTRIALAIGARGLINIQYVIHAGRLYVLEVNPRSSRTVPFLSKVTGVPMV
                     KLATNIMLGKSLQEQGYEGGLWPRQPLIAVKAPVFSMAKLRGVEVHLGP"
BASE COUNT         1425 a         2409 c         2297 g         1326 t
ORIGIN      
        1 agctactcgg caactccaac cattcgggcg cgtagctcgg aaccggcgag accttgtgcc
       61 aatgatcgac caggtactgc acgtcgccgt cttcgatgaa cgcgccctgg atgcggaccg
      121 gcttggcagc atccggcggc aagaagagca tgtcgccacg acccaggagc cgctcggcac
      181 ccggcatgtc gaggacaacc cggctgtcag tcagcgacgt gactgcgaag gcgactctgg
      241 ccggtacatt tgccttgatc aggccggtca gaacgtcgac cgatggccgc tgcgtcgcga
      301 tgatcaggtg gattccggcc gcccgaccca tctgcgccag ccggcagagc acggtctcga
      361 catcgtccgg ggcagtcatc atcaggtcgg ccagctcgtc gatgatcacg acaaggtacg
      421 ggatctcctc ggcacccgcc tcatcggcga ccttcatgcg atagccgtcg aggttgcgca
      481 ctcccatctt cgagaagagc gtgtagcgtc gctccatctc ctgcaagacc agtcgaagcg
      541 caccgacgac cttgtccatc tcggtcacga ccgggcattg cagatgcgga acgccgttgt
      601 agccgatcag ctcgaccatc ttcgggtcca tcatcagcat cttgagttcg ttcggccgct
      661 tgaaggcaag gaaggtcgag atgatcgagg tcaggcagac cgatttgccc gacccggtcg
      721 cgccggcgat gagcaggtgc ggcatcttgg tcagatcgtg gataacatac ttgccgttga
      781 cgtcgcggcc gagcggaatc ggcagcttga acttgccgtg cgcgaaacgc tccgactcca
      841 gcgtctcgcg gagtccgacg gtcgcgacct tcgcattcgg aatctccaga ccgacccggg
      901 ccaggccggg cacgggcgcc tcgatccgga tgctcggagc ggcaagggcg agcgccaggt
      961 cattttgcag ctcggtgatg cgtcgcacct tggtgcccag acccggctcc agcgtgaatt
     1021 gggtcaccgc gggtccagga ttgatttcgc gaacacgagc ctcaaccttg aagctggtca
     1081 gcgtctcttc gatgcgcctg gccttggcgg tgaggtcgcc cttgtcgagc gtgacctgat
     1141 cgtagaggtg ccattgcgcg atgtccggca gggccaggat ctcgtcgctc ggcttgggac
     1201 cgcccacgac cttcgcgacc gcgtccttcc ccttcttgac gattgattct ttttttgggc
     1261 tattgatcac cagggagggc gccgatgcgg gcaccgactc cggcttggcc gcctcaaccg
     1321 gcggttcgat gacggctgga atcgtgtcga aaacgtccgt gtcctcgatt ggagggagcg
     1381 gcttgtagat tgcccggccg atgcgctcca gctcacgata gagcagaagc ggattcgagt
     1441 tggttgcgac cagcacagca gccgacccga tcaggaaaag gccgatcgcg gccgcgtaat
     1501 cgccgacgag tgctcggctg tagtcgtacg tcgtgccgcc gatcaaccca ccgcgctcgc
     1561 tacgcgttcc tgtcgctgcc gcctgcgtca acccagtgac cgacagccca aaggcgactc
     1621 cggcgacgac ctggcggatc tgaatgaatc gaccctgccg atttcgaaag cactggtagc
     1681 tgagcagcat cgtcagcaca ggaatgacgt atggcgcccg gccgcagagc caggcaagga
     1741 agcggtcgac gacttcgacc aggacgccgt tcgattcgcc ggccagcact gtccaggccg
     1801 tgatcaagcc gatcagcgct gataccgcgc cgagaagatc ccgattgaat atcgacgacg
     1861 aggggagaaa tccgccgagc tcagagatcc gcccctgtgc aacgctgagc gcgctctgcc
     1921 gcggatcggc gccctgcttc gacttgggtt tcggcgattt cgccccagac cggagcggcc
     1981 gaccggctgc acgcgttgcc attcatccgc catttttcgc agcgccggtt cttcggcgca
     2041 cccaatgcga ggtgtacgta cgggaatttc attgtacacc gtcataccca gaattacgca
     2101 agtcctacct ttgactgctc gacccccgga cttctatact gtgtttctgg agtgagagga
     2161 accccgcttt tgccgtcgcc ccagaccaca cgccctgaca cggagatagc gcctcgagat
     2221 cgatcgattt cggggtcttt ttttgcgcaa cgcctggaga agctgccatg accagcctcc
     2281 tgctgcgtaa tggccgacta atcgatccgg cacttggcct cgaccagacc ggtgatttgc
     2341 tcatcgtcga tggccggatt gcgagcgtct cgagcgggcc gaacgcgaca acggccgatc
     2401 gtgaagtcga ttgctcggga tccatcgtct cgccgggttg gatcgacatg catgtccacc
     2461 tccgcgtgcc cggccaggag cacaaggaga ccatcgagac cggcactgcc gcggctgcgg
     2521 ccggcggatt taccgcgatc gcggtcatgc caaacacaga gccgtctctg gactcagtca
     2581 agacgattca ttggctgctc gacgagtgcc gggaccgcgc tgttgtccgg gtcctgccca
     2641 tcggcgcgat caccatcggc cgggcgggcc aagagctcgt cgacttcgac gcgctggccg
     2701 acctcggcgt cgtcggcttc tcggacgacg ggatctcgac cgccaattcg gccctgatgg
     2761 ccgaagccct cgcggcaagc aagcgcatcg gtatccccat catggtccat tgcgaggacc
     2821 cctacctcgt cggcggcgtg atgcatgagg gcgaagtcag tgcgcgcctc ggcttgcccg
     2881 ggatcatggc cgcagccgaa gagtcgttca ttgcacggga ctgcctgctc gcccacgaga
     2941 ccggcggttg gctgcacgtt ttacacgtga gcaccgggat cggcctccag atcgtcaagc
     3001 aggccagagc gctcggcgcg cacgtgacgg ttgaagtgat gccgcatcac ctggtcatga
     3061 ccgaccattg ggttgccggt gaccgcgcgc tgcagaacac aagcgagcgc ggtccgaatg
     3121 ctccggcggc gcattccgat accaaggtca acccacccct gcgcaccgcg gacgataccc
     3181 gcgcccttct cgccgggctg gcgaacgggg atttcgacat cctggcaacg gatcatgccc
     3241 cgcacgccgc gcatgagaag cgagacgttc cgatcacgaa ggcggcgttc ggtatgatcg
     3301 gcctcgaatt ggctgtgccg acgatgctgg ccctggttcg cgccggccat ctgacgatga
     3361 cgcaggtgat cgaacgcttc tcaacgcagc cggcccggct gtggcacctg ctgagcggaa
     3421 gaatcgagcc cggcgggatt gcggagctga cagtcatcga cccgaatcac cgctggaccg
     3481 tcactgaaga cgcattgaag tcgaaatcga agaacacgcc cttgctcggc atggaaatga
     3541 ccggtcgagc ggttatgaca ataagcgaag gcaaggtagt gcatgacctc tgcaaatgat
     3601 tcgagcccga agcggtaccg accctcgcat gacgcggcgc tcgccctggc cgatggccgc
     3661 gtcttccgtg gccgcggatt cggcgccgaa accgacgccg gcggcgaagt cgtctttacg
     3721 accacgatga ctggctacca ggaggtctgc actgacccgt cgttcaaggg tcagctcgtc
     3781 tgcatgacct atcccctgat cggcaactac ggtgttgcag cggacgacga cgagtcccgc
     3841 cagccctgga tttcggccct gatcgtgcgg gaacactctg atcacccgag taattggcgc
     3901 tcgaccggca ccatcgacga atacctagag cgctgcggga ttcccggcat ctcaggagtc
     3961 gacacgcggg ccctcactcg ccatattcgt tccgtcggcg acacacgggc agtcctggtc
     4021 cggaatgcgg ccgatttgac cgacgaggag ttggtcgcgc gggccaaaca cgcgccgctg
     4081 ccgggcgaac agccggtagt tgaagaggtc tcgaacgatc atgtcatgac tgaaggacca
     4141 gacgatggac cgcacgtcgt cgtcctcgac tgcggcgtca agcggaacat cgtccgctcg
     4201 ctcttgaatc gcggtgctcg ggttacgagc gtgccatatg ggacgccttt tgccgacatc
     4261 gcggctctcc agcccgacgg tgtaatcgtc tcgcccggac ctggcgatcc ggcgaacctc
     4321 gacgacggac tggacgttgt ccgctcactt ctcgatcaaa agttgcccta cttcggcatt
     4381 tgccttggtc atcaattgct ggcccgcgct atcggcgcgg atacggtcaa gctcaagttc
     4441 ggtcaccgcg gcggcaatca tccggtgctc gacacacgca gcgatcacgt ctcgatcacg
     4501 gcgcagaacc atggctatgt cgtcaagacc gactcactgc cgaacgggtc cggctggaag
     4561 gttgcgctag tcaatctgaa tgacgggtcg gtcgaaggct tttcgcacga atcgctgccg
     4621 gtgctgtcgg ttcaattcca cccggaggcc agccccggcc cgatggacaa cggctatttg
     4681 tttgatcagt tcctcgatat ggtgaaagag cgagctacgg ctcactaggg gagatcgtgc
     4741 gtgtctggcg caatcaagag cgtattggtg ctgggctccg gcccgatcgt gatcgggcag
     4801 gctgccgaat ttgactatgc cggcacgcag gcctgcacag ccctgcggga agagggcatc
     4861 cgtacgatcc tgatcaactc caatcctgcc acgatcatga ccgacgagga cgtcgccgac
     4921 gcggtctaca tcgagccgct gacgccggaa gtcgtccgcc gcgtcatcca acgcgagaag
     4981 cccgacgggc tgctgccgtc gctcggcggc cagaccggcc tgaacctcgc cgtccaggtt
     5041 gcggagcagg gcatcctcga tcgctacgga gtgcgcttgc tcggcacaca acttgagtca
     5101 atcaaggccg ccgaggatcg caagctgttc aaggagctcc tcgagtcgat cggagagccg
     5161 gcgatccagg ggcaaatcgt gacgtcgctt gaggaagcac atgccttcgc cgagctcgtc
     5221 ccattcccgc tcgtgatccg accggccttc acgctcggag ggaccggcgg cggcattgcc
     5281 accaacgcgc aggaactgga cacgatcgtg accagcggcc tcggcgcgag cccgatccac
     5341 caggtgctgc tcgagcgctc cctccagggt tggaaggaga tcgagtacga ggtgatgcgg
     5401 gacagcaacg acacctgcat cacgatctgc aacatggaga acatcgaccc gatgggcgtc
     5461 cacacgggcg acagcatcgt ggtggcgcca tctcagaccc tctcagacaa ggagtaccag
     5521 atgctccgca cctcggcgct caagatcatc cgggcgctca agatcgaggg cggctgcaat
     5581 atccagtaca cgctcaaccc ggattcgttc gaatacgcgg tgatcgaggt caatccgcgc
     5641 gtcagccgat cgtcggcact cgcgtccaag gcaacgggct acccgatcgc ccgcatggcc
     5701 gccaagatcg cgatcggcaa gcgcctcgac gaaatggtca accaggtgac cggcaaaacg
     5761 accgccgcct tcgagccggc cctcgactat tgcgtggtca agatcccgcg ctggcctttc
     5821 gacaagttcg ccaaggccga ccgctccatc ggcacgcaga tgaaggcgac cggcgaagtc
     5881 atggccatcg accggtcctt cgaaggcgcg ttgatgaagg cgattcggtc gctcgagacc
     5941 aatgccaatg acctcggctg ggaggacaag aattggacat cactcgacaa gctcgaagcg
     6001 ctgattcgcc ggccgaatga cgtccggttg tgggcggtgg cggcggccat gcgtcggggc
     6061 gtctgcgccg acgacatcca tgcctggagc aagatcgacc tctggttcct ggacaaaatc
     6121 gcccacctga tcgatctgga gcgccaaatt gcggccggac cgctcaccga cgacctgctc
     6181 tgggaatcga agcgatcggg attctcggac gaccaaattg gccgcttggc cggaacatct
     6241 gccgccgacg tacgcgcgcg gcgcgaagcg gtcggcatga acccggttta caagatggtc
     6301 gacacctgcg ccggcgagtt cgcctccaac acgccgtact tctactcaac ctacgaggaa
     6361 gaggacgagg cgatcccgac cggcggcaag tcagcagtgg tcgtcggttc cgggccgatc
     6421 cgaattggtc agggcatcga gttcgactac tgctcggtcc aggcggccaa agcgctccag
     6481 gacgccggct acgactcgat catgatcaac tcgaatccgg agacggtctc gactgacttc
     6541 gacgcatccg accgactcta cttcgaaccg ctcgatgtcg agagtgtcca cgaggtgctc
     6601 aagcacgagg gccaggtcga ggcaggaatg ccgccggtta tcgtgcagtt cggcggacag
     6661 accgcgatca atcttgccga gccactttcc gaacggggcg tgacgatctt cggcagctcg
     6721 caggacacga tcgacatcgc cgaggaccgc gagcgtttcg aggccttcct gcgcggactc
     6781 ggcattcccc aaccccacgg cgcggcggtc agcacactta ttgacgccac cgatgtcgcc
     6841 gagcgaatcg gataccctgt gctcgtccgg ccgtcgtacg tcctcggcgg ccgtgcgatg
     6901 gaggtcgtct acaacgagga ccacctcgcc cgctacatcc gcaacgccac gaacatgaac
     6961 ccggatcacc cggtgctggt cgacaagtac ctgcttggca aggaggtcga ggtcgacgcg
     7021 atctgcgacg gcgaggaggt actgatcccg ggcgtcatgg agcatatcga gcgcgccggc
     7081 gtccactccg gcgactcatt cgcggtctac cccggcatcc ggctttatcc ggccgagatc
     7141 gacacgatcg tcgagtacac gacccgaatt gcgttggcga tcggcgcgcg cggcctgatc
     7201 aacatccagt acgtgatcca cgccggccgg ctctacgtgc tcgaggtcaa cccgcgctca
     7261 tcgcgcacag ttcccttcct gagcaaggta acgggcgttc cgatggtcaa gctcgcgacc
     7321 aacatcatgc tcggcaaatc gctgcaggaa cagggttacg aaggcggact ctggccccgg
     7381 cagccattga tcgcggtcaa ggccccagtc ttctcgatgg ccaagctgcg gggtgtcgag
     7441 gttcacctcg gacccga
//
LOCUS       DATAOZ010000388         4146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7527326, whole genome shotgun sequence.
ACCESSION   DATAOZ010000388 DATAOZ010000000
VERSION     DATAOZ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7527326"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VHR64_09890"
     CDS             <1..516
                     /locus_tag="VHR64_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303182.1"
                     /translation="LKPMGAWEQMEADYAVLGLSARYHPLGLLRSRLPSHYVTTVDIE
                     TLPSGRVIQIAGLIVCRQRPGTAKGVQFLLLEDEVGLVNVVVQPWLYEKRRLTVRGEP
                     FLVITGEIQHQGGAINVVARDVVPLDQARRHYDDISFETIAMPSARDLEDPVATSDNT
                     SSISPASHNYY"
     gene            complement(513..2846)
                     /locus_tag="VHR64_09895"
     CDS             complement(513..2846)
                     /locus_tag="VHR64_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX3303183.1"
                     /translation="MTASSIERSRLDIGGLDCADCARTLERRLGETPGVSSCAVRFET
                     GTADVAFDPGVVSDGVLRSRIRELGYSVGRPTDRRSEREFMVSGMDCADCARTLQASI
                     AALPGVSSAEVVFGAGTLRLEADPSLVPDAQIRAAAQRLGYRAEPVESRSSDETKTRS
                     IPRRTWEIAIAAALVLVALGLSAFTDLVVLPASLHVIAAVVAGYPVARAALISIKVRR
                     ADMNLLMTIAAIGAMALQDWAEASTLMVLFAIGLALQSATIDRTRGAIRELMSIAPQT
                     ARIARKGRIETVPVEEVATDDIVDIMAGERVPIDGVVLSGTSALDQSAITGESLPRPV
                     EVGSEVISGSVNGDGHLTVRATAPGTDTTVSRIIHLVETAHQSKAPVQALVDRFAAIY
                     TPAVVIGAIALAVGGLLFSSGDTDWIYRALILLVVACPCALVISTPVAIVSALGRASR
                     MGVLFKGGSALEALAGVKQIAFDKTGTLTHGRPEVVAVNSVNGLDRDEVVALAAALEH
                     GTTHPIGRGIVRAADGLPRPAVADLQVRAGRGIVGQVGTDAVQVGSSALFEDIPTELG
                     RRIEDGRALGQSVVLVGRNGVAEAAITVVDSIRPISPAVISSIESLGLHPMLLSGDVA
                     AVANRIGAETGISDVRADLLPADKASFIAEANRTTPTAMVGDGINDAPALAAARVGIA
                     MGAGGSAAAIEAADVALMGDDLTALPRSITLARSTRAIILQNIAFALAMKLGFLVLTV
                     SGFTSLWLAVVADVGASLLVTLNALRLMRGQDKTHSI"
     gene            2912..3118
                     /locus_tag="VHR64_09900"
     CDS             2912..3118
                     /locus_tag="VHR64_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303184.1"
                     /translation="MLVSQGVKDTPAIAPGADDIELSHLPELMGNGGLADADRGAEIA
                     NAKFNGIERGHDSKPGRIGHQTKK"
     gene            3433..3505
                     /locus_tag="VHR64_09905"
     tRNA            3433..3505
                     /locus_tag="VHR64_09905"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3466..3468,aa:Thr,seq:tgt)
     gene            3536..3616
                     /locus_tag="VHR64_09910"
     tRNA            3536..3616
                     /locus_tag="VHR64_09910"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3570..3572,aa:Tyr,seq:gta)
     gene            3658..3733
                     /locus_tag="VHR64_09915"
     tRNA            3658..3733
                     /locus_tag="VHR64_09915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3691..3693,aa:Thr,seq:ggt)
     gene            3847..>4146
                     /locus_tag="VHR64_09920"
     CDS             3847..>4146
                     /locus_tag="VHR64_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX3303185.1"
                     /translation="MGKAKFERTKPHLNVGTIGHVDHGKTSLTAAITKTLSLKGEAAF
                     RSFDSIDNAPEERERGITIAISHVEYETDKRHYAHVDCPGHADYIKNMITGAAQMD"
BASE COUNT          808 a         1298 c         1306 g          734 t
ORIGIN      
        1 ctcaagccga tgggcgcgtg ggagcagatg gaggcggact acgcggtgct tggcctctcc
       61 gcccgctatc acccgctcgg cctgctccgg agccggctgc cgtcccatta cgtgaccacg
      121 gtcgatatcg agacgttgcc cagtggccgg gtgatccaga tcgctgggct gattgtttgc
      181 cggcagcgac cgggcacggc caagggcgtc cagttcctgt tattggagga cgaggtcggg
      241 ctggtcaatg tcgtggtgca gccgtggctc tacgagaaga ggcggctgac cgtgcggggc
      301 gagccattcc tggtgattac cggcgagatc cagcaccagg gcggcgcaat caatgttgtt
      361 gcacgcgacg tcgtcccgct cgatcaggcg cggcgacact acgacgacat ctcattcgag
      421 acaatcgcca tgccatccgc gcgcgacctg gaagatcccg tcgccacgag cgacaacacg
      481 agctcaatca gcccggcatc gcacaactac tactaaatgg agtgcgtctt gtcctggcca
      541 cgcatcagtc gtaacgcgtt gagggtcacg aggagcgatg cgccgacatc ggcgacgacc
      601 gcgagccaga gactggtgaa gccgctgacg gtcagcacca gaaagccaag cttcatcgcg
      661 agcgcgaagg cgatgttctg caggatgatc gcgcgggtcg agcgggcgag tgtgatcgaa
      721 cgcggcagag cggtcaggtc atcgcccatc agcgcgacgt cggctgcttc aatggctgcc
      781 gcggagccac ccgcgcccat cgcgattccg acccgtgctg ccgccaatgc cggcgcgtcg
      841 ttgatgccgt cacccaccat cgccgtcggc gtagtccggt tcgcctcggc gatgaaggat
      901 gccttgtccg ccggcaggag gtctgcgcga acatcagaaa tcccggtttc ggcgccgatg
      961 cgattggcaa ctgccgcgac atctcccgac agcagcatcg gatggaggcc aagcgactcg
     1021 atcgatgaga tcaccgctgg actgatcgga cggatcgagt cgaccaccgt gatcgccgcc
     1081 tcggctacgc cattgcgccc aaccagcacc acagactgac cgagtgcccg gccatcttcg
     1141 atgcgtctgc caagctctgt cgggatgtcc tcgaaaagtg ccgacgagcc gacctgaacg
     1201 gcatcggtgc cgacctggcc gacgattcca cggccggctc gaacctgcag atctgcaacc
     1261 gccggacgtg gcaagccatc ggccgcgcga acgatgccgc gaccgatcgg gtgcgtcgtg
     1321 ccgtgctcga gcgcggcagc cagtgccaca acctcgtcgc ggtccaggcc attgaccgaa
     1381 ttgaccgcga ctacttccgg gcggccatgg gtcagcgtgc cggtcttgtc aaacgcaatc
     1441 tgcttgacgc cggccaacgc ctccagcgcg ctgccaccct tgaaaagcac gcccatccgc
     1501 gacgcgcggc caagagccga cacaatcgcg accggtgtcg agatcaccag cgcacaggga
     1561 cacgcgacga cgagcaggat cagcgcacgg taaatccagt cggtgtcccc cgagctaaac
     1621 agcagccctc cgaccgccag cgcgatcgct ccgatgacga cggccggcgt gtagattgcg
     1681 gcgaatctgt cgaccagcgc ttgtaccggc gccttcgact ggtgcgcggt ttccaccaaa
     1741 tgaatgatcc ggctgaccgt cgtgtcggtg ccgggagctg tcgcccgtac agtcagatgc
     1801 ccatcgccgt tgacgctgcc cgagatgacc tcgctcccca cctcgaccgg cctgggaagc
     1861 gattcgccgg tgatggccga ctggtcgagc gccgatgttc cgctcaggac gactccgtcg
     1921 atcggcactc gctcgcccgc catgatgtcg acgatgtcat ctgtcgcaac ttcctcgact
     1981 ggaaccgttt cgattcgccc tttccgggct atgcgggcag tttgcggtgc gatcgacatc
     2041 aattcccgga ttgccccgcg tgtccggtcg atcgtggccg actggagtgc gagcccgatc
     2101 gcgaagagga ccatcagcgt gctggcctcg gcccaatcct gcagtgccat ggcgccgatg
     2161 gcggcgatcg tcatcagcag attcatgtct gcgcgacgca ccttgatcga aatcagtgcc
     2221 gcgcgggcaa ctgggtaccc cgcaaccact gccgcaatta catgaaggga agctggcaaa
     2281 acgacgagat cggtgaatgc gctcaggccg agcgcaacca gtacaagggc cgccgcaatc
     2341 gcaatttccc atgtgcgtcg cgggatcgac cgtgttttgg tttcgtcgga cgacctcgat
     2401 tcgaccggct cggcgcgata tccgagtcgt tgggcggccg cccggatctg cgcatcgggg
     2461 accaggctcg gatcagcctc gagtcgtagt gttcccgccc cgaacacgac ttctgccgag
     2521 ctcacgcccg gcagcgcggc gatgctcgcc tggagcgtcc gggcgcagtc ggcgcagtcc
     2581 atcccggaga ccatgaactc gcgctcggac cggcggtcag tcggtcgccc gaccgaatat
     2641 ccgagctcgc ggatgcgcga tcgcaggaca ccgtccgata cgacgcccgg gtcaaaagcg
     2701 acatccgccg tgccggtttc aaatcgaaca gcacacgacg agacgcccgg cgtctcaccg
     2761 agccgccgtt cgagtgtccg ggcgcagtcg gcgcagtcga gcccgccgat atccaggcgg
     2821 gatcgctcta tagatgatgc tgtcatgtgc gcgtcaccgt attcagtcga tggcccacgt
     2881 gttcacgtgc ctggcgataa atggacataa catgctcgtc agtcagggag taaaagacac
     2941 gccggccatc gcgccgggcg cggacgacat cgagctctcg catttgccgg agctgatggg
     3001 aaacggcgga ctcgctgatg ccgatcgtgg cgctgagatc gcaaacgcaa agttcaacgg
     3061 tatcgagcgc ggccacgatt cgaagccggg tcggatcggc catcagacca agaagtgagg
     3121 acaaccggga gaggtcatcg ggatcaggtt gggtcgcccg tgcctggtgg acggcttcaa
     3181 gatgaacgat gcgctcgtcg caatcgttgt cgggagtggc ggcaaattga tcctgttttc
     3241 gtggccgagc catgcttaaa cctcgccacc attatacatg aatagttatt cagttgtata
     3301 cttaaacctt gcgggaagac cggcaagttg gcatctcttg agacaacgca gaaatccccc
     3361 gatttcgcgt tgacgctcat cgaagccgtt cgtatactcc ccgttggcgc agcttttggt
     3421 cgcgtccggt ttgccgacgt agctcaattg gcagagcagc tgttttgtaa acagcaggtt
     3481 gcgggttcga gtcccatcgt cggctcttct cgcgcctcgc gaggtaccgg tacacgggca
     3541 gttgccagag tggacaattg ggcctgactg taaatcaggt gcgtaagctt cggtggttcg
     3601 aatccgccac tgcccactcc aaacgcgtgt gccgcgctgc gcgagcagcg ggatcgagcc
     3661 cacatagctc agtcggtaga gcactttctt ggtaagaaag aggtcatcgg ttcaagtccg
     3721 attgtgggct ccagggtctt tgacggggag actccgcgag gagtctctcg ttgcgcagga
     3781 gagccgggag ttccggcgag acactgacca ggcaccggcc ggtcagtaaa ggagattaac
     3841 caagcaatgg gcaaggcgaa attcgagcgg accaagccgc atttgaacgt gggcacgatt
     3901 gggcatgtcg atcatggcaa gaccagtttg acggcggcga tcacgaagac gttgtcgttg
     3961 aagggggagg ccgcgtttcg cagcttcgat tcgatcgaca atgccccgga ggagcgggag
     4021 cgggggatca cgatcgcgat cagccacgtc gagtacgaga ccgacaagcg ccactacgcc
     4081 catgtcgatt gtcccgggca tgccgactac atcaagaaca tgatcaccgg ggcggcccag
     4141 atggat
//
LOCUS       DATAOZ010000389         8825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8317016, whole genome shotgun sequence.
ACCESSION   DATAOZ010000389 DATAOZ010000000
VERSION     DATAOZ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8825
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8317016"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /gene="glmU"
                     /locus_tag="VHR64_09925"
     CDS             <1..1299
                     /gene="glmU"
                     /locus_tag="VHR64_09925"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX3303186.1"
                     /translation="CGRPMIDYVLDAAEIPALARTVTVLSPTLNDNPEMVAHLRQRVG
                     DKLGIAIQYEALGTGDALRAAIPLLDDVTSIIVMFADHPVLTRYRMVELSESLRPADA
                     VVSLLTCVVDSAAGYGRIERTVDSQIQRIVERKDDDPGLRVGPTEINSGMMAIDVEWL
                     RSAIHRLRPSHATGEYYLTQLVELAVGDGRRVTSVQGDPAGLVGVNDRVDLAHAESLL
                     QERIQQQHLRAGVTLVAHQTTVIEHGVVIGPDTTVLPGCLIKSGTVIGSGCEIGPHTV
                     LDRARIGDGCRITASFVTDSEVMDGSDVGPYSHVRGGAMISPGVHIGNFAEIKNSTLA
                     TGVRMGHVSYVGDASIGERTNIGAGAITCNYDGVNKHRTDVGADVFIGSDTMLVAPVS
                     IGNGAATGAGSVVTRDVPAGERVVGVPARPLPNRTRENGE"
     gene            1304..2242
                     /locus_tag="VHR64_09930"
     CDS             1304..2242
                     /locus_tag="VHR64_09930"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX3303187.1"
                     /translation="MDGRLQVFTGNAHPQLAQDIMRELEAPLGRAVVGQWRNGETRVK
                     LEENVRGSDVFVIQSMSEPANHHLMEMLMMIDAARRASAARITAVIPYYAYAKQEKKT
                     SGREPISAKLVANLIVTAGADRVLTVDLHAPAIEGFFDIPVDHLRATPLLAERIKRTG
                     ETNLVAVSPDSGGVARADNFRGRLKAPLAIISKRHPEPDATEVLEMVGDVEGKLAVIV
                     DDMISTGGTLALAANMLRERGARKVVAAATHGVFAENAFELLAASAIERVFVTDSLLL
                     PHGQECLAVEVVSIAPMLAEAIMRIHKDLSISALFS"
     gene            2239..2988
                     /locus_tag="VHR64_09935"
     CDS             2239..2988
                     /locus_tag="VHR64_09935"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX3303188.1"
                     /translation="MKPEPQFIKLDAVESTMTEAADRLSREEIGPWTTIIARHQTSGR
                     GRLDRRWEAEPGDALLATVVVPLAVPPERIGLIALATGLALADAISKWNVPVTLKWPN
                     DIYVDDRKLGGILIRTRLDATVTALIGIGINLSSVPDGHLSAATCLAAHAKDVPQPLS
                     LAKAIVRQLQFRADQLEARMYQLIVDDWTSRAVWIGETVAVASGTPITGTFIGVDELG
                     RMLISLDSGTQAIAEGDLQRGPRRSLYLGMK"
     gene            3032..4216
                     /locus_tag="VHR64_09940"
     CDS             3032..4216
                     /locus_tag="VHR64_09940"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX3303189.1"
                     /translation="MSKSVILGGARTPFGRLSGTLASKTSVELGGIAIGAAIDRSGVS
                     RDDVEHVIMGQVLQGSVGQIPARQAAFRAGLDKTVTAETINRVCGSGMRAVELADLQI
                     RAGMHSVIVAGGMESMTHAPYFVRNARNGYRMGNGAFEDMMIVDGLFCAIDCVQMGAH
                     GDKVAAELGINREEQDDWALRSHQRAIAAIDGGRMAEEIAPVEIVGRKETITVDTDEA
                     PRRDTSAEALAKLKPAFTGAGTVTAGNAPGVNDGAAALVIASDEWATRNDKPFLARIL
                     SHGEAAWDAPYLAKTPAMAGEIALERAGLKSSDIDLFEINEAFANVALNASRMLGADP
                     ERVNVNGGAVALGHPIGASGARIVLTLAHELKRRGGGLGLAAICSGGGQGDAIVIEVP
                     AA"
     gene            4213..4764
                     /locus_tag="VHR64_09945"
     CDS             4213..4764
                     /locus_tag="VHR64_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX3303190.1"
                     /translation="MSDRFPGAHILEVNGICPKIAEDVFIAPGAVIVGDVEIGPGSSV
                     WFNAVLRGDVAPIRVGAGTNVQDLSLLHVDRGNPCIVGDNVTIGHSAIVHGTVLEDDV
                     TIAMGAIVLSRSHIGRGAVIAAGAVVSEGMQVEAGALMAGVPAVLKRTLNPERREQLA
                     AIGGLYVENARRFRTTLKSIEGM"
     gene            4768..5541
                     /locus_tag="VHR64_09950"
     CDS             4768..5541
                     /locus_tag="VHR64_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX3303191.1"
                     /translation="MAVDVMIDGGIATVTLNRPDALNAFNTQQLKDAVAILASLKENR
                     SVRAVILTGAGERAFAAGADIKEMVGLDRDGAMNFGRLGHAVTRGVETLPQPVIAAVN
                     GFAMGGGCELALAADVRLASENAVFGQPEVSLGIPPGWGGTQRLVRAVGPGMAAEMIL
                     TGRRVKADEAVRIGLVNAVYSLADLMPKAREMAQMIAKNSPLAVRASKRLMQLAFNGQ
                     VASGLDTELHAFADSFGTADQVEGMSAFVEKRSPNYADE"
     gene            5547..6473
                     /locus_tag="VHR64_09955"
     CDS             5547..6473
                     /locus_tag="VHR64_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303192.1"
                     /translation="MSDPGRFVEIHAATPAAAACAAVLDRAGVSVRIIDSEPRTVGLA
                     AVSRELLSELGLDGSRLGKPVETIIDRTLAEDDAMDKVREPGSVSLVDRLEIVESLTS
                     GIERATDVENESRAFSIGAGEFRLGDPDLLDDGILPHEKTLECILLDWDGARIDRASW
                     FRLTGEGLADVDATASILTSHVQTSLILTVPMASIVETSISVVDVLARLLAHPSVRNM
                     VPEMEPRTASTRLMRTGEPVHITLRGGARVRIGAAAGLADPVRLDRELRSGTVAAEQI
                     AQAVTGGRLTLARLSRIGRVWSAIESDQAVSS"
     gene            6574..>8825
                     /locus_tag="VHR64_09960"
     CDS             6574..>8825
                     /locus_tag="VHR64_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX3303193.1"
                     /translation="MQEELKAIADLLQPQEAGRRFLRPSALGRFISYDQCHRLFRLDL
                     YDRETRNAIHSAAGTQPELLSPALSESGDEWERAIESALIDAGWFVTNLEDSTRAGLA
                     LHYFHLTEDGQRKIVLQSELDGVLDGWTICAKPDLVVIDKTDPGTPRLLIADLKSSRA
                     VKFEHRLQVALYGMMAKQLFPEAEITQAVLYREPVDPVEIWTDEERQHQQDARELLNL
                     EHGALLAVADRPDRYEEQVRRTVLAPGSLADRIALAPFFELPYHLASKCDGCEFNEFC
                     LYSARKTHDLSLIPYLTERNKRMLESNGVETIEDLRTVRESSRYEKLQVAPSLGFRMD
                     ELIRRAESFTQWQSGEAVDTRMPEIGYSSLPAFGPDLHPNLIRVFIDLQRDASSGRIY
                     LLGALINCLSDGTEPGARESSVVKIADRPIQNDADEANLVREWIRDVLIAVQNTMSAD
                     ADGLISAPVHFYVWDQAQISVLQNLVNRQAQNVIGIEAVMALMMQPAAFDTDNVSTVA
                     DEVQHQRALPMLCQSLQSVASYLGFRWRDDLKSQFHYKMFDALTQERDEEGAHLVPDR
                     SRFRSEIPSDYVHRAWGIDDPGQDEDPEAEPRRSWSLYQRPTIEQLRDFQQQRLEAVK
                     FVTSQLRTNRQTTKSRFDLNVLARLNFRPDKPIEAIREFLSVERHVELSEWRKVHSLG
                     INTRVKMGATLVARYFDEDQDVDVRVKMRDARQKQATWDALGDKKGELSPEQKKQLNY
                     DLDATTLRLRV"
BASE COUNT         1689 a         2602 c         2902 g         1632 t
ORIGIN      
        1 tgtggccgac ccatgatcga ctacgtgctc gacgcggccg aaattcccgc acttgcccgg
       61 accgtgacgg tgctcagccc aacattaaac gataacccgg agatggttgc tcacctcagg
      121 caacgcgtcg gagacaagct cggcatcgcc attcagtacg aggcgctcgg gaccggggac
      181 gctctgcgcg ccgcgattcc cttgctcgac gacgtgacca gcatcattgt catgttcgcg
      241 gatcatccag tcctgacgcg gtaccgaatg gttgagctca gcgaatccct acgccccgcc
      301 gatgcggtcg tcagcctgtt gacctgcgtg gtcgactccg cagccggtta cggacgaatc
      361 gagcgtactg tggatagcca gatccagcgg atcgtagagc gaaaggacga tgatcccggc
      421 cttcgggtcg gtcccacgga aatcaacagc ggaatgatgg cgattgatgt cgagtggttg
      481 cgttccgcga tccatcgact caggccaagt cacgcgaccg gcgagtacta cctgacgcaa
      541 ctggtcgagc tcgccgttgg cgacgggcgt cgggttacca gtgtgcaggg agaccccgca
      601 ggtctggttg gcgtcaatga ccgagtcgat ctggcgcacg ccgagtcact gctgcaggag
      661 cgcatccaac aacagcacct gcgtgccgga gtgacattgg ttgcgcatca aaccacggtc
      721 attgagcacg gagttgtgat tggaccggat accactgtcc tgccgggttg tctgatcaag
      781 tcaggtacgg tcattggctc aggctgtgag atcggtccgc atactgtcct cgatcgtgcc
      841 cgcattggcg atggttgccg gatcactgcc tcgttcgtga ccgattccga agttatggat
      901 ggttctgatg ttgggccata cagccatgtt cggggcggcg ccatgatttc gcccggtgtg
      961 cacataggca attttgcgga gatcaagaac agcacccttg ccaccggcgt tcgcatgggc
     1021 cacgtcagct atgtaggaga cgcgtcgatc ggcgagcgaa cgaatatcgg tgcgggcgct
     1081 attacctgca actacgacgg tgtcaacaag catcgcactg atgtcggtgc ggacgtcttc
     1141 atcggcagcg atacgatgct ggtcgccccg gtctcgattg gaaacggggc ggcgactggg
     1201 gccgggtcgg tcgtcaccag ggatgtcccg gccggagaac gcgtcgtagg ggtacctgcg
     1261 cgcccattgc caaatcgaac gagggagaac ggggaataac gtgatggacg gcagattgca
     1321 ggtgttcacc ggcaacgcgc atccacaatt ggcccaggac atcatgcgcg agttggaagc
     1381 tccgctcggg cgggcggttg tcggccagtg gcgcaacggc gaaacccggg tcaagctcga
     1441 agagaatgtc cgcggatctg acgttttcgt gatccagtcg atgagcgagc cggcgaatca
     1501 tcatctcatg gagatgctga tgatgatcga cgccgcacgc cgggcatctg ccgcgcgcat
     1561 aaccgccgtt attccgtact acgcctacgc caagcaggag aaaaagacct ccgggcggga
     1621 gccgatctcg gccaagctgg tcgcgaactt gatcgtaacg gccggggcag accgcgttct
     1681 aacggttgac ctgcacgcac cggcgatcga gggcttcttc gatattccgg tggatcattt
     1741 gcgcgccaca ccgttgctgg ccgagcgcat caagcggacc ggcgagacca acctggttgc
     1801 ggtcagcccg gattccggtg gagtcgcgcg ggccgacaat ttccggggtc gcctgaaggc
     1861 gccgctggcg atcatttcta agcggcatcc cgaaccggat gccaccgaag tgctcgagat
     1921 ggtcggcgac gtcgaaggca agttggcggt catcgtggac gacatgattt cgaccggagg
     1981 cacgctcgcg ctcgccgcca acatgctgcg agagcgcggc gcccggaaag tcgtcgcggc
     2041 tgccacgcac ggggtcttcg ccgaaaatgc cttcgaactg ctcgctgcgt cggcaatcga
     2101 gcgcgtcttt gtcacggact cattgctatt gccacatgga caggaatgcc ttgccgttga
     2161 agtcgtgtcc atcgccccga tgctggctga ggcgatcatg cggatccaca aggacctgag
     2221 catcagcgcg ctcttcagtt gaagcccgag ccccaattca taaagctcga cgcagttgag
     2281 agtacgatga ccgaagcggc cgatcgtctt tcgcgcgaag agattggccc ctggaccacg
     2341 atcattgccc gccaccagac aagcgggcgg ggccgtctgg atcgccggtg ggaagccgaa
     2401 ccaggcgacg ccttgcttgc gacggtagtg gtcccgctag cagtcccgcc ggaacgaatc
     2461 gggctgatcg cactggcgac cgggctggct ctcgcggatg ccatctccaa gtggaatgtt
     2521 ccggtaacgc tcaagtggcc caatgacatc tacgtcgatg atcgaaagct cggcgggata
     2581 ctgatccgga cgcgcttgga tgccacggtg accgccctca tcggaattgg gatcaacttg
     2641 agctccgtcc cggacggtca cttgagcgcg gcgacgtgcc tggccgcgca tgcaaaggat
     2701 gtcccgcagc ctctttccct ggccaaagcg atcgtccgtc agctccagtt cagagcagat
     2761 caactggaag ccaggatgta tcaactgatc gttgacgatt ggacatcgcg ggcagtctgg
     2821 attggtgaga cggtcgctgt ggcatcagga acgcccatca cgggtacgtt catcggagtt
     2881 gatgagctcg gccgcatgct catctcgttg gacagcggta ctcaggcgat cgcggaaggc
     2941 gatctgcagc gcgggccacg ccgctcccta tacttgggta tgaaataaac gcgctcggtc
     3001 cgggaaattg cggaccagag aggaagtaat cgtgtcgaaa tcagtaatcc tcggcggcgc
     3061 ccgaaccccg tttggccgtc tcagcgggac gcttgccagc aagacatctg ttgagctcgg
     3121 cggcatcgcc attggcgccg cgatcgatcg gtccggggtc tcccgcgacg atgtcgagca
     3181 cgtgatcatg gggcaggtgc tgcagggcag cgtggggcaa atcccggcgc ggcaggcggc
     3241 attccgggcc ggtctcgaca agaccgttac cgccgagacg atcaaccgcg tttgcggctc
     3301 cggcatgcgc gcggtggagc ttgccgacct gcaaatccgg gccgggatgc attcggtcat
     3361 cgtggccggc ggtatggaga gcatgaccca cgcgccgtac tttgtccgca atgcccgcaa
     3421 cgggtatcgc atgggcaatg gcgcgttcga ggacatgatg atcgtcgatg gcttgttctg
     3481 cgccatcgat tgtgtccaga tgggtgcaca cggcgacaag gtggcagcgg agctgggcat
     3541 caatcgcgaa gagcaggacg actgggcgct tcgctcgcat cagcgggcga tagccgcgat
     3601 cgatgggggc cggatggccg aggagattgc cccggtcgag atcgtgggcc gcaaggagac
     3661 gatcacggtc gataccgacg aggcaccgcg gcgggatacg agcgccgagg cattggcgaa
     3721 gctgaagccg gccttcaccg gagcaggtac ggtcacggcc ggaaacgcgc ccggcgtcaa
     3781 tgacggagcg gcggcgctcg tgatcgcgtc cgatgaatgg gcgacacgca acgacaagcc
     3841 gttcctggcc cggattctga gccacggcga ggcagcatgg gatgcgccgt atctcgccaa
     3901 gactcctgcc atggccggcg agattgcgct ggagcgagcg ggtctgaagt cctccgacat
     3961 cgacctgttc gagatcaacg aggccttcgc caacgtggcc ctgaacgcgt cccggatgct
     4021 cggcgctgat cctgagcgtg tcaacgtcaa cggcggagcg gtcgcactcg gacacccgat
     4081 cggagcaagc ggcgcgcgga tcgtgcttac cctggcgcac gagttgaagc ggcgcggcgg
     4141 tggacttggt ctcgccgcga tctgctcggg tggcgggcag ggtgatgcga tcgtcatcga
     4201 ggtcccggcc gcatgagcga tcggttcccc ggagcgcaca ttctcgaggt caacggcatc
     4261 tgcccgaaga ttgccgagga tgtcttcatc gcgccgggcg cagtgattgt gggcgacgtc
     4321 gagatcggac ccggcagcag tgtctggttc aatgccgttc tgcgcggcga cgtggctccg
     4381 atccgggtcg gtgctggcac gaacgtccag gatctctcgc tgctccatgt cgaccgcggg
     4441 aatccgtgca tcgtcggcga caacgtcacg attgggcata gcgccattgt ccacggcacc
     4501 gttctcgagg acgacgtcac gatcgcgatg ggcgcgatcg tgctttcccg gtcgcacatc
     4561 gggcgagggg ctgtgatcgc tgccggggcg gttgtgtcgg aagggatgca ggtggaggcg
     4621 ggtgccctga tggcgggtgt gccggcggtc ttgaagcgga ccctgaatcc cgagcgccgc
     4681 gagcaactcg cagcgattgg cggactgtac gtagagaatg cgcgccggtt ccggaccaca
     4741 ttgaaatcaa tcgaggggat gtaggggatg gcagtcgacg taatgatcga tggtggaatc
     4801 gcgactgtca ccctcaaccg gcccgacgcc ctcaacgcgt tcaacacgca gcaactgaag
     4861 gacgcggtcg ccatccttgc ctcgcttaag gagaatcgat cggtacgcgc ggtgatcctg
     4921 accggcgccg gtgagcgcgc atttgccgcc ggagcggata tcaaggaaat ggtcggactt
     4981 gaccgcgacg gcgcgatgaa tttcggccgg cttggtcatg ccgtcacccg gggagtcgag
     5041 acgctcccgc agccggtgat tgcggccgtc aatgggttcg cgatgggtgg cggctgcgag
     5101 ctcgcactgg cggccgacgt ccgactcgcc agcgagaatg cggttttcgg gcagccggaa
     5161 gtcagcctcg ggataccgcc aggctggggc gggacgcagc ggcttgtcag ggcggttggt
     5221 ccggggatgg cggccgagat gatcctgacc gggcgtcgcg tcaaggcgga tgaagccgtg
     5281 cggattggac tcgtcaacgc cgtctattcg ctcgccgatc tcatgccgaa ggcgcgggag
     5341 atggcgcaga tgatcgcgaa gaatagtccg ctggcggttc gggcgtccaa gcggctgatg
     5401 cagctcgcgt tcaatggtca ggtcgcgtcg ggactcgaca ccgagcttca cgcgtttgct
     5461 gactcgttcg gaaccgcgga tcaagtcgaa ggcatgtccg ctttcgttga gaagcgatct
     5521 ccgaattacg cagacgagta gtgcttgtgt cagatccggg acgattcgtc gagatacatg
     5581 cggcgacgcc tgcggctgcg gcctgcgcgg cggtgctgga ccgggcaggt gtatctgttc
     5641 gaattatcga ttctgagcct cggactgttg gcctggcagc agtctcccgg gaactcctca
     5701 gcgagctcgg actggatggc tcgcgccttg gcaagcccgt cgagacgatc atcgaccgca
     5761 cgctggctga agacgatgcg atggacaagg tgagggaacc gggcagcgtg tcccttgtcg
     5821 ataggctcga aatcgtcgaa tcgctcacca gtggaattga gcgcgcaacc gacgttgaaa
     5881 acgagagtcg cgcgttctcc atcggggccg gcgaattccg actcggcgat ccagacctcc
     5941 tggacgacgg gatattgccg catgagaaga cgctcgagtg catcctgctc gattgggacg
     6001 gcgcacgaat cgaccgggcc tcctggttca ggttgacggg cgaaggcttg gcggacgttg
     6061 atgcgacggc atcaatcctc acgtcgcacg ttcagacatc attgatcttg acagttccca
     6121 tggcgtcgat cgtcgagacc tcgatttcgg tcgtcgacgt gctcgcccga cttctggctc
     6181 atccgtcagt caggaacatg gtgcctgaga tggagccgcg aactgccagc acacgcttaa
     6241 tgaggaccgg ggaaccggtt cacatcacgc tccgaggtgg cgccagagtt cggattggcg
     6301 cggcagctgg gctggccgac ccggtccgtc tcgatcggga gcttcgatcg ggcacggtcg
     6361 cggcggagca gattgcccag gccgtaaccg gcgggcgcct cactctggcc cgtcttagcc
     6421 gaatcgggcg agtgtggtcg gcgattgagt ccgaccaggc ggtgtcttcc tgacgtggat
     6481 cggtaccttt cgggacgctg acgttttccc cgctgaggtg cacaattgtg agtagccccg
     6541 gacgaacccc ggcgaacagc gggagcgcgc ttcatgcagg aagaactcaa ggcgattgcg
     6601 gacctcctcc aaccccagga ggcaggacgt cgattcctgc gacctagcgc tctcggtcgt
     6661 ttcatcagct acgaccaatg ccatcgactc ttccggctcg atctgtacga ccgcgagacg
     6721 cgaaacgcca tccactcggc ggccggcact caaccggagc ttctctcgcc ggcgctctca
     6781 gaatcgggcg atgaatggga gcgggcaatt gagtcagcgc tgatcgacgc cggctggttt
     6841 gtgaccaacc tcgaagactc cacgagggcc ggacttgcgc ttcattactt tcatctgacc
     6901 gaagatgggc aacgcaagat cgtcctccag agcgagctcg acggcgtgct ggatggctgg
     6961 actatctgcg ccaagcccga cctcgtcgtc atcgacaaga cagatcccgg cactccgcgc
     7021 ctgttgatcg ccgatctcaa gtcgtcgcgc gcggtcaagt tcgagcatcg gctgcaggtc
     7081 gcgctctacg ggatgatggc gaagcaattg ttccccgaag cggaaatcac gcaggcagtg
     7141 ctctatcgcg agccggttga tcccgtggag atctggaccg acgaggagcg gcagcatcag
     7201 caggacgcgc gcgaattgct caacctcgag cacggggccc tgctggcggt ggctgatcgg
     7261 ccagatcgat acgaggagca ggtccggcgg acggtgctcg cccctggttc gctcgccgat
     7321 cgcatcgcac ttgccccgtt cttcgagctc ccgtaccacc ttgcgtccaa gtgcgatggc
     7381 tgcgaattca atgagttctg cctgtatagc gcccggaaga cgcatgatct ctcacttatt
     7441 ccctacctga ctgagcgcaa caagcgcatg ctcgagtcga acggagtgga aacgatcgag
     7501 gatctgcgga ccgtcaggga atcctcccgg tacgagaagc ttcaggtggc gccatcgctc
     7561 ggattccgaa tggacgagct gattcgccgg gccgagtcat tcacgcagtg gcagtcgggt
     7621 gaggccgtcg atacgcggat gccggagatc gggtacagca gcctgccggc attcggtccg
     7681 gacttgcacc cgaacctgat ccgtgtcttc atcgatctgc agcgggacgc gagctcgggg
     7741 cggatttact tgctcggcgc attgatcaat tgcctgtcgg acggtacgga gccgggcgcc
     7801 cgagagtcga gcgtggtcaa aatcgctgat cgtccgatcc agaacgatgc cgacgaggca
     7861 aatctggtcc gcgagtggat ccgcgacgtt ttgattgcgg tccagaacac gatgtcggcg
     7921 gatgccgatg gacttatctc ggcaccggtc catttctacg tctgggatca ggcgcagatc
     7981 agcgtgctgc aaaacctggt caaccgccag gcgcagaacg tcatcggtat cgaggcagtc
     8041 atggcgctga tgatgcaacc ggccgccttt gacactgaca acgtgagcac ggttgcggac
     8101 gaggtccagc atcagcgggc cttgccgatg ctctgccagt cgttgcaatc cgtcgcctcc
     8161 tacctcggat ttcgatggcg ggatgatctg aagtcgcagt tccattacaa gatgttcgac
     8221 gcgctgacgc aggagcgcga tgaagaaggc gcgcaccttg tccccgatcg gtcccggttc
     8281 cgaagcgaaa ttccgagtga ctacgttcac cgagcgtggg gaatcgatga tccagggcag
     8341 gacgaagatc cggaggcaga accacgacgg agctggtcgc tctatcagcg gccgacgatc
     8401 gaacagcttc gtgatttcca gcagcagcgg ctcgaggcgg tgaagtttgt gacctcgcag
     8461 ctccgcacga atcgccaaac gaccaagtcg cggttcgacc tcaacgtgct cgcgcgcctc
     8521 aacttcagac cggacaagcc aatcgaggca atccgcgagt ttctgtcagt ggagcgtcat
     8581 gttgagctca gcgagtggcg taaggttcat tcgctcggca tcaatacgcg cgtcaagatg
     8641 ggcgcaacgc tggttgcccg ctacttcgac gaggaccagg acgtcgacgt gcgcgtcaag
     8701 atgcgcgacg cccgtcagaa gcaggcgacc tgggatgcgc tgggagacaa gaaaggtgag
     8761 ctcagtcctg aacagaagaa gcagctcaat tatgacctcg acgcaacgac gcttcggctg
     8821 agggt
//
LOCUS       DATAOZ010000390         5178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8051191, whole genome shotgun sequence.
ACCESSION   DATAOZ010000390 DATAOZ010000000
VERSION     DATAOZ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8051191"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VHR64_09965"
     CDS             <1..623
                     /locus_tag="VHR64_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303194.1"
                     /translation="GSALTLAVLIGIGSGAAIARNWVGTSGVLSVLVSVGMALPSFWV
                     GATLVLLFAVNLGWLPSSGGGSARALILPAITLALQPTAVIARLTETQLREALGSDFV
                     RTARGKGLTEPAVFLRHALRPGLGPILAFIGIQIGFLIGGAVIVEGVFAYPGVGLLAL
                     NAVKDRDLPIIQAFVVVSALLISLTTMLIDIVSRLIDPRIAAGSTA"
     gene            623..1510
                     /locus_tag="VHR64_09970"
     CDS             623..1510
                     /locus_tag="VHR64_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303195.1"
                     /translation="MSTITQPRLCQSSAREWRLPSSGGTAAAVFAGLLIALGVLGPTL
                     LAHDPDKISLTDRLMPPLGFGGTWAHPLGTDQLGRDLLARVVAGARISLIVALSATVL
                     AGTIGVTIGLVGGYFGGRLDRLAVWLGDVQLAIPFVVVAIGVSASLTPSAANVVVILG
                     ITGWATYARVARLAAQPLRHAGYIDAARLAGAGSGRILFRHLLPVIAPTLLAIGGQQA
                     GAMMLYEAALSYLGLGVPAGTITWGGMVADARETAQTAWWATVVPGLAIVLVVIGFNA
                     TSAVISRRVLNGRSTRRHA"
     gene            1507..3018
                     /locus_tag="VHR64_09975"
     CDS             1507..3018
                     /locus_tag="VHR64_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3303196.1"
                     /translation="MIPAQMLNRRMLLQATTLGFGSLALPRLTSLAQTPGPVDEITID
                     LSSEPASLAPSQVYEVNGWSVVHSIFDAPFEYDANGQMVMVAAESLSQVDDTTFEIKL
                     RPGITFHDGTALTSQSLVASYQQIVNPDTGSSIAGNFSTITAVDAVDDVTVRISLNAP
                     SPWLPAQIAVWMLCLSPNATTASDTSEKPVGTGPYSFVEWKRGESITLAANPNYNNPA
                     KGTPIANRVVFRFVTEAATRDADLQSGTAQIVRAVPPDQIKQVEDSGAQVMQVPLSCV
                     AFVRIATDVKPFDDVRVRQAINYAVDVDAIREALLAGTGRRLPNLYVPGGMGYDESLA
                     PYSYDPDKAQSLLSEAGVSGLSTTIAATNSERKDLVEAIAGYLSNAGIATTVEIQEVA
                     TFNGQWTDVSAPPLRYATWRPMFDPFNLLNLVFSEKGFLSRHENPDIQALIDQAAVAT
                     DPATRAGLYQQLGKVMFDQPAALYLWDVTALYGTAAGLNWSPRPDDAIVPTLR"
     gene            2990..3640
                     /locus_tag="VHR64_09980"
     CDS             2990..3640
                     /locus_tag="VHR64_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX3303197.1"
                     /translation="MTPSSPPSADLDLTRPADLDSLIRGRRSVRKLLNDPVPPEVIRD
                     AIEAAGWAPSPHGRQPWRFAVIESTERKQALADAMADAWQEQLALDGQNQAVIGIRRR
                     KSHERIVSAPAIIVVCLRLTDLDPYPDADRTEAERVMAIQSLGAAVQNFLLHIYASGY
                     DAGWMCAPLFCPEVVRDVLGLPDDFIPHAMLPVGRAAADPVRRPRRPVDELIASWE"
     gene            3645..4487
                     /locus_tag="VHR64_09985"
     CDS             3645..4487
                     /locus_tag="VHR64_09985"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX3303198.1"
                     /translation="MSTDHRLLMIFAHPDDESFGFAGTMLALADAGVKMSLIVATRGE
                     EGEILVPGLATKENLGEVREGELRAAMEIVGIDDIHFLGFRDSGMAGTPPNHDPRAFV
                     NQDVNKVAATIADSIAELRPTIILTYGPDGIYAHPDHIMVNTVAWPAVLKAGEDGRWK
                     TPNLYFSAAPKERMRRMAELPNGPFASMDPDLVASLGMPASEITTWIDTTPFADRKRE
                     ILMAHRTQVGEEGPFNQLPPEMRRIWFSVETMRTIPLPWNPDPDDVIAELLPAAAHDH
                     PFRA"
     gene            complement(4625..>5178)
                     /locus_tag="VHR64_09990"
     CDS             complement(4625..>5178)
                     /locus_tag="VHR64_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX3303199.1"
                     /translation="MATIWKIDEAHSSVEFVVKHMMVSRTKGRFTKFDGTINLDEENL
                     ANSSAKVEIDVASIDTHDEKRDAHLRSADFFDVEQFPTITFVSTEVLPRGGDKFQLVG
                     DLTVKGVTEKVVIEVESTGIGTSPWGQKVAGFEAKTDIDRKDYGLTWNVALETGGFLV
                     GDNIKINLEVEAIQQAAAEAEAA"
BASE COUNT          855 a         1747 c         1644 g          932 t
ORIGIN      
        1 ctggctcggc tttgaccctc gcagtgctga ttgggatcgg gtcaggagcg gccattgccc
       61 gcaactgggt tggcaccagc ggcgtgctgt cggtgctggt ctcagtcggg atggcattgc
      121 cgtccttttg ggtcggcgcg acgctggtcc tgctcttcgc cgtcaatctc ggctggttgc
      181 cgtcgtctgg aggcggatcg gcgcgtgcct tgatcctccc tgcgatcacc ctggcccttc
      241 agcctacggc cgtgatcgcc cgcctgaccg aaacgcagct ccgcgaggcg ctgggatcag
      301 acttcgtccg caccgcccgg ggcaaggggc tgaccgagcc ggcggtattc ctgcgccatg
      361 cgcttcggcc cgggctgggg ccgatcctcg ccttcatcgg catccagatc ggcttcctga
      421 tcggcggcgc ggtcatcgtc gagggggtct tcgcctatcc cggcgtcgga ttgctggcgc
      481 tgaacgccgt caaggatcgt gatctgccga tcattcaagc attcgtcgtc gtctccgcgc
      541 tgctaatcag cctgaccacg atgctgatcg acatcgtgtc gcgcctgatc gacccgcgaa
      601 tcgccgccgg cagtacagcg tgatgagcac gatcacccag ccccggctgt gccagagctc
      661 cgcgcgcgaa tggcggttgc cctcgtcggg cggaactgcc gccgccgtct tcgccggcct
      721 gttgatcgcg ctcggcgtgc tcggacccac gctgctcgct cacgacccgg acaagatctc
      781 tctgaccgac cgactcatgc caccgctcgg attcggtgga acctgggcgc acccgctcgg
      841 gacggaccag ctcggtcgcg atctccttgc gcgggtcgtc gcgggcgccc gtatctccct
      901 gatcgtggcc ctctccgcga ccgtgctcgc cggaacgatc ggcgtcacga ttggcctggt
      961 cggcggctac ttcggcggtc ggctcgatcg actggcggta tggctcggcg atgtccagct
     1021 ggcaattccg ttcgtggtcg tcgcaatcgg tgttagcgca tcgctcactc cttcggcggc
     1081 caatgtggtc gtgattctcg gaatcaccgg ctgggccacg tatgcccggg tcgctcgcct
     1141 ggccgcgcag cccctccgcc acgccgggta catcgacgcc gcgcgcctgg ccggtgcggg
     1201 cagtggccgg atcctgttcc ggcacttgct cccggtgatt gcgccgaccc tgctggccat
     1261 cgggggccag caggcgggcg cgatgatgct ctacgaagcg gcgctcagct acctgggtct
     1321 cggcgttccg gccgggacca tcacctgggg cggaatggtg gccgacgcgc gggaaacggc
     1381 ccagacggca tggtgggcga ccgtcgtgcc cggcctggcg atcgtgcttg tggttatcgg
     1441 gttcaacgca accagcgccg tgatctcgcg gcgggtgctc aatggtcgtt cgacaaggag
     1501 acacgcatga ttccagccca gatgctcaat cggcgtatgt tgttgcaggc aacgacactc
     1561 ggattcggct cgctggcact gcctcggctt acatcgctgg cccaaacgcc cggtccggtc
     1621 gatgagatta cgatcgacct cagctccgag ccggcgtcgc ttgcgccgag ccaggtctat
     1681 gaggtcaacg gctggtcggt tgttcactcg atcttcgacg cgccgttcga gtacgacgcg
     1741 aacggccaga tggtgatggt cgcggccgag tcgctgtcac aggtcgacga tacgaccttc
     1801 gagatcaagc tgcgcccggg tatcacattc cacgacggca cggcgctgac ctctcagtcc
     1861 ctggtcgcga gctaccagca gatcgtcaat ccagataccg gcagctcgat cgccggcaac
     1921 ttctcgacca tcacggccgt ggacgcggtc gatgacgtaa cggtccggat ctcgctcaac
     1981 gcgccatcgc cctggctacc ggcccagatc gccgtctgga tgctttgcct atcgccgaat
     2041 gcgacaaccg cgagcgacac ctcggaaaag ccggtcggga ccggtcccta ctccttcgtg
     2101 gagtggaagc gcggcgagtc gatcacgctc gcggccaacc cgaactacaa caatccggcc
     2161 aagggcacgc cgatcgcgaa ccgggtcgtc ttccgcttcg tgaccgaggc cgcaacccgc
     2221 gatgccgatc tccagtccgg gaccgcccag atcgtgcgcg cggtgccgcc ggaccagatc
     2281 aagcaggtcg aggactccgg cgcacaggtc atgcaggtgc cactctcctg cgtcgccttc
     2341 gtgcggatcg cgaccgacgt gaagcccttc gacgacgtcc gcgtgcggca ggcgatcaac
     2401 tacgcggtcg atgtcgatgc aatcagggag gcgctcctgg ccggtaccgg tcggcgactg
     2461 ccgaacctgt acgtgcccgg tgggatgggt tacgacgagt cgctcgcgcc ctactcgtac
     2521 gacccggaca aggcgcagtc gctgctctcg gaggcagggg tcagcgggct ctcgacgacc
     2581 atcgccgcca ccaatagcga gcgaaaggat ttggtcgagg cgattgccgg ctatctgtcc
     2641 aacgccggta tcgcgacgac ggtcgagatc caggaggtcg cgacgttcaa cgggcagtgg
     2701 accgacgtat ccgcaccgcc gcttcgctac gccacgtggc gaccgatgtt cgacccgttc
     2761 aacttactca acctcgtctt cagcgagaag ggcttcctct cccgccacga gaaccccgat
     2821 atccaggcgc tgatcgacca ggcggcggtc gcgaccgatc ccgcgacccg agccggcctg
     2881 tatcagcagc tcggcaaggt catgtttgat cagccggccg cgttgtacct ttgggacgtt
     2941 acggcgctgt acggaacagc cgccggcctg aactggagcc cgcgcccgga tgacgccatc
     3001 gtccccaccc tccgctgatc tggacctgac ccgaccagcc gacctcgatt cgctgatccg
     3061 gggtcggcga tcggtgcgca agttactgaa cgacccggtc ccacccgaag tgatccgtga
     3121 cgcgatcgaa gcggccggct gggcgccatc gccgcacggc cgccaaccgt ggcgcttcgc
     3181 cgtgatcgaa tcgaccgagc gcaaacaggc gttggccgat gccatggcag atgcctggca
     3241 ggagcagctg gcgctcgacg ggcagaacca ggcggtgatc ggcatcagac ggcgcaagag
     3301 tcatgagcgg atcgtctcgg cgccggcaat catcgtggtc tgcctccgcc tgaccgacct
     3361 cgatccgtat ccggatgccg atcggacgga ggccgagcgg gttatggcaa tccagagcct
     3421 gggcgccgcc gtgcagaact tcctgctcca catctacgcc agcgggtacg atgccggctg
     3481 gatgtgcgcg ccgctcttct gcccggaggt cgtgcgcgac gtcctcggcc tgccggacga
     3541 cttcattccg cacgccatgc tgccggtcgg acgcgccgcc gccgatccgg tgcggcgtcc
     3601 gcgccggccc gtcgacgagt tgattgcatc ctgggagtga ccacttgagc accgaccacc
     3661 gcctgttgat gatcttcgcc cacccggacg atgagtcgtt cgggttcgct ggcacgatgc
     3721 tggccctggc agacgccggt gtgaagatgt cgctgatcgt cgccacccgc ggcgaggagg
     3781 gcgagatcct ggttcccggc ctggcgacca aagagaacct tggtgaggtg cgagaggggg
     3841 agttgcgggc ggcgatggag atcgtcggga tcgacgacat ccacttcctc ggcttccgtg
     3901 attccgggat ggccggtacg ccgccgaacc acgatccacg cgccttcgtc aaccaggacg
     3961 tgaacaaggt cgccgcgacg atcgccgata gcatcgccga gttgcggcca accatcatcc
     4021 tgacctacgg gcctgatggg atctacgccc atcccgatca catcatggtc aacacggttg
     4081 cctggccggc tgtcctaaag gcgggtgagg atggccgctg gaagacgccg aatctctact
     4141 tttcggcagc gccgaaggag cggatgcgtc gcatggccga actgccgaac ggcccgttcg
     4201 cgtcaatgga tccggacctg gtcgcctcat tgggcatgcc ggcgtccgag atcacgacct
     4261 ggatcgatac cacgcccttc gccgatcgca agcgggagat actgatggcg caccggaccc
     4321 aggtcggcga ggaggggccg ttcaaccagc tgccgccgga gatgcggcga atctggttct
     4381 cggtcgagac gatgcggaca atcccactgc catggaatcc cgaccccgat gacgtcatcg
     4441 ccgagctcct gccggccgca gcccacgacc acccgttccg cgcctgagaa tacgaacggg
     4501 atgtgcgttt gaaacgtagc gcgcggccgc cgtgccccgc taaattgact aacgcggttg
     4561 cgctgaaaac acgaacggcg gctggataac cagccgccgt cgcgcgttga gaaaggaggg
     4621 aagcctacgc ggcctcagcc tcagcagccg cctgctggat ggcctcgacc tcgaggttga
     4681 tcttgatgtt gtcaccaacc aggaacccgc cggtctcgag ggcgacgttc caggtcaggc
     4741 cgtaatcctt gcggtcaatg tcggtcttcg cctcgaaccc ggccaccttc tgaccccacg
     4801 gcgacgtgcc gatgccggtc gactcgacct cgatcaccac cttctcggtg acgcccttga
     4861 cggtcagatc gcccaccagc tggaacttgt cgccgccccg cgggagaacc tcggtgctga
     4921 cgaaggtgat cgtcggaaac tgctccacgt cgaagaaatc ggcgctgcgc agatgcgcgt
     4981 cacgcttctc gtcgtgcgtg tcgattgacg ccacgtcgat ctcaaccttc gccgaggagt
     5041 tcgccaggtt ctcttcgtcc aggttgatcg tgccatcgaa cttcgtgaag cgacccttgg
     5101 tccgcgaaac catcatgtgc ttgacgacaa actcaaccga agagtgcgct tcatcaatct
     5161 tccaaatcgt tgccataa
//
LOCUS       DATAOZ010000391         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3316882, whole genome shotgun sequence.
ACCESSION   DATAOZ010000391 DATAOZ010000000
VERSION     DATAOZ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3316882"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..514)
                     /locus_tag="VHR64_09995"
     CDS             complement(149..514)
                     /locus_tag="VHR64_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303200.1"
                     /translation="MSTVAFYSGELMSPEAIRAAYPEARFIARASVLINGQPVADRFA
                     PVIDQTVWGIAVDIGSDLPGETILATADDGRSLRVVLAEPLLSGDPATVLANARYWEL
                     PPAFVAALQSVVLPGQVEE"
     gene            complement(574..>1544)
                     /locus_tag="VHR64_10000"
     CDS             complement(574..>1544)
                     /locus_tag="VHR64_10000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX3303201.1"
                     /translation="FGITIARQIAECWDRMGRPVGFSIREYGSGVGGLAYDILAGLSD
                     AAPDLFASITYRLVERNRVRQAEALTAFRDMGLIDRIVDESDATLEPIAGVILANEVS
                     DAFPAHRLVFEAGRFREAWVVGDGDGFQFENGPVSDEGELAVQQLQDDGVRLVEGGVY
                     DVSPASAAWFGGACRHLERGYALVIDYGYEAPELYQHHRLTGTLRGYFGHTATEDPFA
                     NIGAQDLTVHVDFTALQRAGEAAGLTFAGLTTQGAFLAGLGLGEHLLQLQSEPDTTMS
                     EYLATQAVVARLIDPGGLGRFRVLMMARDAPIDPPLQGFSIAPPSF"
BASE COUNT          289 a          496 c          533 g          226 t
ORIGIN      
        1 cgccaagcag gccgacccga atcgtgagcg ttgttggctg gatctcgacg ctggtgcgct
       61 ggagcacgcc gtcggccgga tgggcggaga cggttgaacc gcaaaggcaa agcgcaatga
      121 tcgcggggag gaatgtgcgg accaggtgct attcctcgac ttgaccgggc agaacgaccg
      181 attgcagggc ggcgacgaag gcgggcggca gctcccaata gcgggcattg gccagtaccg
      241 tggccggatc gccggagagc agcggctcgg caagcacgac ccgcagcgag cggccgtcat
      301 cggcggtcgc aaggatcgtc tcgccgggga gatccgagcc gatatcgacg gcgatgcccc
      361 aaacggtctg gtcgatgacc ggggcaaatc gatcggcaac cggctggccg ttaatcagga
      421 ccgacgcgcg cgcgatgaac cgggcctccg gataggcagc ccggattgcc tcagggctca
      481 tcaactcgcc tgaatagaat gcaaccgtcg acacgcactc ccccaccgct cgataccgca
      541 ccgccgttgt tgcgctgatt ctacctgtcg tgatcagaac gacggcggag cgatgctgaa
      601 gccttgcagg ggcggatcga tcggcgcgtc cctggccatc atcaggacgc gaaaccggcc
      661 gagtccgccc ggatcgatca gccgcgctac gaccgcctgg gtcgccaggt attcactcat
      721 cgtggtatcc ggctccgatt ggagctgaag caggtgctcg ccgagaccga gcccggccag
      781 gaatgcgccc tgcgtggtca ggcccgcgaa agtgagtccg gcggcttcgc cggcacgctg
      841 gagggcggtg aaatcgacgt ggaccgtcag atcctgcgcc ccgatgttgg cgaacggatc
      901 ctcggtcgcc gtatggccga aatagccgcg cagcgtgccg gtcaggcgat ggtgctggta
      961 gagctccggc gcctcgtagc cgtaatcgat gaccagcgca tagccgcgtt cgagatgccg
     1021 gcaggcgcct ccgaaccaag cggccgatgc cggcgagaca tcgtagaccc cgccttcgac
     1081 gagccggaca ccgtcatcct gaagctgctg gacggccagc tcgccctcgt ccgagaccgg
     1141 tccattctcg aattggaagc cgtcgccatc gccgacgacc catgcctcgc ggaagcgccc
     1201 ggcctcgaag accaggcgat gagcggggaa ggcatccgag acctcgttgg cgaggatgac
     1261 gccggcgatc ggctcgagcg tcgcgtcgga ctcgtcgaca atccggtcga tcaggcccat
     1321 gtcgcggaag gcggtcaggg cttcagcctg gcggacacgg ttgcgctcga ccagacgata
     1381 agtaatcgag gcgaacaggt cgggcgcggc gtcggagaga ccggccagga tgtcgtaggc
     1441 gaggccgccg accccgctgc cgtattcccg gatcgagaag ccgaccggtc ggcccatccg
     1501 gtcccagcac tcggcgatct ggcgggcgat cgtgatgccg aaga
//
LOCUS       DATAOZ010000392         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4896148, whole genome shotgun sequence.
ACCESSION   DATAOZ010000392 DATAOZ010000000
VERSION     DATAOZ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4896148"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /gene="sufD"
                     /locus_tag="VHR64_10005"
     CDS             <1..1166
                     /gene="sufD"
                     /locus_tag="VHR64_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX3303202.1"
                     /translation="RTDLRTLKLDRYAPFTGVGETVSSLDGVLAKGSIANAGQAGVVV
                     QRDAATVYATANAKAVPDGVIFTDLTTAVDQHADLVREYLMTKAVPVSFGKFEALHAA
                     FWQGGSFLYVPKGVSVELPFRSFVLAQTAGSSVFNHTLIVLEEHAEAFLVDAYQSETQ
                     EQPTFASGVVELIVGKGATLRYVQLQDYGRNVWNIMTERAVLDRDATLNSLHVTLGSK
                     WTKNSIGSHLRGENALAEMLGIYFGDSDQFFDHHTWQLHESPYATSDLEFKGALRDSA
                     RSVYSGLIKVYEGAQKTDAYQQNRNLVLSREARADSIPNLEIGANDVRCTHGATISQV
                     VPEHVFYLQARGIPRTEAQKLIVEGFFRPVIDRIPVEEIQDFLENAIARKVGL"
     gene            1241..1756
                     /locus_tag="VHR64_10010"
     CDS             1241..1756
                     /locus_tag="VHR64_10010"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3303203.1"
                     /translation="MDLLDRLLGHDEWTTAELLRRCGELTDFQMRERFDIGHETIQAT
                     LVHMIDNVGNWTELMRTSHVEDRIDGWAEMGVPSLIAEHERSYAEFAALAREVRDADR
                     WDDTWVDTLDDPPQSKTYGGAILHVITHNMHHRGEVLHMLSRLGLTDLPEGDLLSWEA
                     GHIAEKGVMTQ"
     gene            1753..>2508
                     /locus_tag="VHR64_10015"
     CDS             1753..>2508
                     /locus_tag="VHR64_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3303204.1"
                     /translation="MTARTSNLPALDVATIKADFPILNQLPTDGRKPLVYLDSAASSQ
                     KPQSVIDAVDHFYAEANANIHRGVYQLSERATGLYEQARHIVADFINAADARELIFTR
                     NTTEAINLVAQAWGRANLKPGDIILVTEMEHHSNLVPWQLIAEQTGARIKAIPLTDDH
                     TLDIDAYHTLLAGQPKVVAVTHVSNSLGTINDVAAIARDAHTVGATVVIDAAQSAPHL
                     PIDVQAIDADFLAFSGHKMLGPLGSGALYGKLAL"
BASE COUNT          472 a          807 c          799 g          430 t
ORIGIN      
        1 ggcggaccga tctccgcacg ctcaagctgg atcgctatgc gcccttcacg ggtgtcggcg
       61 agacggtttc gtcgctcgac ggcgtcctcg ccaagggctc gatcgccaat gccggacagg
      121 ccggcgtggt cgtccagcgc gacgccgcga ccgtttacgc cacggctaac gcgaaggctg
      181 tcccggacgg cgtgatcttc accgacctga ccacggcggt tgatcagcat gcggacctcg
      241 ttcgcgagta cctgatgacc aaagccgtgc cggtcagctt cggcaagttc gaggcgctcc
      301 atgccgcctt ctggcagggc ggatcgttcc tttacgtgcc gaagggtgtc agcgttgagc
      361 tgccgttccg ctcgttcgtc ctggcgcaga cggccggctc gtcggtcttc aaccacacgc
      421 tgatcgtgct cgaggagcat gccgaggcgt tcctggtcga tgcctaccag tcggagacgc
      481 aggagcagcc gacctttgcc tcgggcgtgg tcgagctgat cgtgggcaag ggcgcgaccc
      541 tgcggtacgt tcagctccag gactacggcc gcaacgtctg gaacatcatg acggagcggg
      601 cggtgctcga tcgcgatgcg acgctgaact cgttgcacgt cacgctcggc tcgaagtgga
      661 ccaagaactc gatcggctcg cacctgcggg gcgagaatgc gctggccgag atgctgggca
      721 tctacttcgg cgacagcgat cagttcttcg atcaccacac atggcagctc catgagtcgc
      781 cgtatgcgac cagcgacctc gagttcaagg gtgcactgcg agacagcgcg cgctcggtct
      841 actccggcct gatcaaggtc tacgagggag cgcagaagac cgacgcctac cagcagaacc
      901 gcaacctggt cctgagccgg gaggcgcggg ccgactcgat accgaacctc gagatcggcg
      961 cgaacgatgt tcgctgcacg cacggcgcga ccatctcgca ggtcgttccc gagcacgtct
     1021 tctacctgca ggcgcgcggt atcccgcgga ccgaggccca gaagttgatc gtcgagggct
     1081 tcttccgccc ggtcatcgac cggattccgg tcgaggagat ccaggacttc ctggagaacg
     1141 cgatcgcacg gaaggtcgga ctctagggta gttggtagat ggacgttgga cgttagtgat
     1201 ccatctacca acgtccaacc tcccacgacc gagaggtgac atggatctgc ttgaccggct
     1261 gcttgggcac gacgaatgga caactgctga gctgctgcgc aggtgcgggg agctcaccga
     1321 cttccagatg cgcgagcggt tcgatatcgg gcacgagacc atccaggcca ccctcgtcca
     1381 catgatcgac aacgtcggca actggaccga gctaatgcgg accagtcatg tcgaggaccg
     1441 gatcgatggc tgggccgaga tgggtgtgcc gtcgctgatc gccgagcacg agcgatcgta
     1501 tgcggagttc gcagcgctgg ctcgtgaagt tcgtgacgcc gaccgctggg acgatacctg
     1561 ggtcgacacg ctcgatgatc cgccgcaatc caagacatac ggcggcgcga tccttcacgt
     1621 catcacgcac aacatgcatc accgcggcga ggtgttgcac atgctcagcc gtctcggcct
     1681 gaccgatctc cccgagggcg atctcttgag ctgggaggcc ggtcatatcg ccgagaaagg
     1741 ggtcatgacg caatgaccgc acgcacgtca aacctaccgg cgctcgatgt cgccacgatc
     1801 aaggccgatt tcccgatcct gaaccagctg ccgacggacg ggcgcaagcc gctcgtctat
     1861 ctcgacagcg cggcaagctc gcagaagccg caatcggtga tcgacgcggt cgaccacttc
     1921 tacgccgagg cgaatgcaaa tatccatcgc ggcgtctacc agctctcgga gcgagcgacc
     1981 gggctgtatg agcaggcgcg gcacatcgtg gccgacttca tcaatgcggc tgatgcgcgt
     2041 gagttgatct tcacccgcaa cacgaccgag gcgatcaacc tggtcgcgca ggcgtggggc
     2101 cgggcgaacc tgaagccggg cgacatcatc ctggtcacgg agatggagca ccactccaac
     2161 ctcgtaccat ggcaactgat cgccgagcag accggcgcgc ggatcaaggc gatcccgctg
     2221 accgatgatc acacgctcga catcgacgcc taccacacgc tgctggccgg tcagccgaag
     2281 gtcgtggccg tgacgcacgt ctcgaacagc ctcggcacga tcaacgacgt ggcggcgatc
     2341 gcgcgcgatg cgcataccgt cggtgcgacg gtcgtgatcg acgcggccca atcggcgccg
     2401 catttgccga tcgatgtcca ggcgatcgat gccgacttcc tggccttcag cgggcacaag
     2461 atgctcggcc cgctcggttc cggcgcgctc tatggcaagt tggcgctg
//
LOCUS       DATAOZ010000393         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6998050, whole genome shotgun sequence.
ACCESSION   DATAOZ010000393 DATAOZ010000000
VERSION     DATAOZ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6998050"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2793)
                     /locus_tag="VHR64_10020"
     CDS             complement(<1..>2793)
                     /locus_tag="VHR64_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX3303205.1"
                     /translation="AGQPAEYKQVTVLFADVVHSMDVAAAVGPERLREIMAELFDRSG
                     AVVRRYDGTVDKFTGDGIMAVFGAPIALEDHALRACMAALQIQVEADKLDADVGSRDR
                     IELRLRIGLNSGQVIAGEIGSTTASYTAIGEQVGMAQRMESAAPPGGVMLSESTARLV
                     EAAVVLEKPELVAIKGADDPVPARRLLAIGEHQARRRSDSRLVGRAWELNTVTALLEE
                     AVAGAGCVVTVVGPPGIGKSRLVRETLAAATSRGIRMFTTYCESHTADLAFHVVARLM
                     RGFTGVEGLGAGEARARIRASLPDADPDDLPLIDDLLGIGDPDAALPDVGPDARRRRL
                     TAVVNAAALSTREPAVCVIEDAHWIDPASESMLADFMAVVSQRPTLMLITYRPEYRGA
                     LSQVPGAQTIALRPLSDTHAAALTTDLMGTDPSLAGLVDRIVDRAAGNPFFAEEMVRD
                     LAEREVVDGQPGAYTLRRDIEDVDVPANLQSAIAARIDRLPVTAKKTLSAASVIGLRF
                     DADLLAELVKEVDIAPLIAAEVVDQVRFTRSAEYAFRHPLIRTVAYESQLKSDRAHLH
                     RRLGAAIEKGGGVDENAALIAHHLEAAGDLHAAFEWHMRAAAWFTFRNFAAAQASWLR
                     ARDVADRLPEDDSGRLSMRIAPRSLLCAHEYRIRSGHADEQFAELKELCAMAGDKRSL
                     AIGLAGVALATQLDLGPRPAAPLTGELIHLLDSIDDTKLTVSLSVAPMTAGIQSGHVA
                     AAHQLAQRVIELTGGKPSLGELITVSPVASALSIRGTARWTMGSPGWRADFDEALRTI
                     AAIPPAFRSGTLWHVYVYSLPNGVLLPTSAVVDNAAEIYAASEQYGERVTVDLAKGAL
                     GMVLIYREGPERDRGVELLEELHDRAQRERPMIAQNMPVIDLHLAREKARRGDFDGAI
                     ELARNAFDDYLQTGDFM"
BASE COUNT          504 a          918 c          874 g          497 t
ORIGIN      
        1 catgaaatcg cccgtctgca ggtagtcgtc gaaggcgttt cgcgccaatt cgatcgcacc
       61 gtcgaagtcg ccacgacgag ccttttctcg ggccagatga aggtcgatca cgggcatgtt
      121 ctgtgcaatc atcgggcgct cccgctgggc gcggtcatgc agctcttcga gtaattcgac
      181 acccctgtcg cgctctggtc cttctcggta gatcaggacc ataccgaggg ctcctttggc
      241 gagatcgacc gtgactcgct cgccgtactg ttctgaggcc gcatatattt cggctgcatt
      301 gtcgacgacg gccgacgtcg gtagcagcac tccattcggc agcgaataca cgtacacgtg
      361 ccagagggtg ccggagcgga atgccggagg gattgcggcg attgtccgca acgcctcatc
      421 gaagtcagct cgccaacccg gcgaacccat cgtccaccgg gccgtgccac gtattgagag
      481 ggccgatgcg accggagata ccgtgatcag ctcaccgaga cttggcttac cgccggtgag
      541 ctcgatgaca cgctgcgcca gctggtgtgc ggctgccaca tggccgcttt gaatgccggc
      601 agtcatcggt gccaccgaaa gcgacacggt caactttgtg tcgtcgatgg agtcgaggag
      661 atgtatcagt tcacctgtca gcggagccgc cggccgcgga cccagatcga gttgggttgc
      721 caacgccacg ccggcaagac cgattgccag ggaacgcttg tctccggcca tggcacagag
      781 ttccttgagc tcggcgaact gttcgtcagc atgaccgctg cggatacggt attcgtgggc
      841 acagagaagg gagcgagggg ctatccgcat cgacagtcgg ccggaatcgt cctcagggag
      901 tcggtccgcc acatcccgag ctcgcagcca actcgcctgc gcggcagcga agttgcgaaa
      961 tgtgaaccac gctgctgccc gcatgtgcca ttcgaatgcc gcatgaaggt cgcctgccgc
     1021 ctcgaggtgg tgggcgatca gcgcagcatt ctcatcgacg ccgccgccct tctcgatcgc
     1081 ggccccaagg cgtcgatgca gatgagcccg gtcggatttc agttgtgact cgtacgcgac
     1141 ggtgcggatc agcggatgcc tgaacgcgta ctccgcggaa cgcgtgaagc gcacttgatc
     1201 cacgacttca gcggcgatca acggagcgat gtccacctct tttacgagtt cagccaagag
     1261 gtcggcatcg aagcgcaggc cgatgaccga cgccgcactc aaagtcttct tcgcggtcac
     1321 cggtaggcgg tcgatgcggg cagcgatcgc ggattgcagg ttggccggca catcgacatc
     1381 ctcgatgtcg cgacgaagtg tgtacgcccc aggctgacca tcgaccacct cgcgctcggc
     1441 gagatcgcgc accatttcct cggcgaagaa cggatttccg gcggcccggt caacgattcg
     1501 gtcaaccagt ccggccagtg acggatccgt acccatcaag tctgtcgtca aggccgcagc
     1561 gtgcgtgtca ctcagcggtc gcagggcgat cgtctgcgcg ccagggactt ggctcaaagc
     1621 accgcgatac tccgggcgat acgtgatcaa catcagcgtg ggtctctggg aaacaacggc
     1681 catgaaatca gcaagcatcg attcgctggc cgggtcgatc cagtgcgcgt cctcaatcac
     1741 gcacacggcc ggttcgcgcg tgctcagagc ggccgcattg accactgccg tcaagcgtct
     1801 gcgccgagca tcgggcccga cgtcgggtag ggcggcgtcg ggatcaccga tgccgagcag
     1861 atcgtcgatg agcggcagat cgtccgggtc ggcgtcgggg aggctggccc ttatgcgtgc
     1921 tcgggcttcg cctgcgccga ggccttcgac gccggtgaag ccgcgcatca gccgcgcaac
     1981 cacgtggaac gcaaggtcgg cggtgtggga ctcgcaatac gtggtgaaca ttcggatgcc
     2041 gcggcttgtc gcggccgcca gcgtttcgcg aacgaggcgg ctcttgccga tgcccggcgg
     2101 accgacgacc gtgaccacac accccgcgcc cgcgaccgcc tcctcgagca aggcggtgac
     2161 ggtgttgagt tcccatgcgc ggccgacgag tcgcgagtcg ctacggcggc gggcctggtg
     2221 ctcgccgatc gccaaaagcc ttctggcagg caccggatcg tcggcgccct tgatcgcaac
     2281 cagttcaggc ttctccaaca ccacagcggc ctcgaccagt cgcgccgtcg attcgctgag
     2341 catcacccca ccgggcggtg cagccgactc catccgctgc gccatgccga cctgctcgcc
     2401 gatggccgtg taactggctg tcgtggaacc gatctcaccc gcgatcactt ggcccgagtt
     2461 caacccgatg cgcagtcgca gctcgatgcg gtcacggctg ccgacgtcgg cgtccagctt
     2521 gtcggcctcg acctgaattt gcaatgcggc catgcacgcg cgaagagcgt gatcctccaa
     2581 cgcaatcggg gcgccgaaca cggccatgat cccgtcgcct gtgaatttgt cgaccgtgcc
     2641 gtcgtagcgc ctgaccactg cacccgatcg atcgaaaagc tccgccatga tctcgcgtag
     2701 ccgctcgggc cctaccgctg cggcgacatc catcgagtgc acgacatcgg cgaacagcac
     2761 tgtcacctgc ttgtactcag caggctgccc cgc
//
LOCUS       DATAOZ010000394         2816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6210779, whole genome shotgun sequence.
ACCESSION   DATAOZ010000394 DATAOZ010000000
VERSION     DATAOZ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6210779"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..510)
                     /locus_tag="VHR64_10025"
     CDS             complement(4..510)
                     /locus_tag="VHR64_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303206.1"
                     /translation="MNIHDPRFSLVFVDCEASGPCPGKGELTEFGAVEYRTRETFHGI
                     LIESAPRDDNPHLSEVTGRRFDPVVVFTAFERWLAEVCTGRPIFISDNPAFDWQWINY
                     GFHHWLGRNPFGHSARRISDFYAGLVGEFWNSQAWKRLRITPHDHNPVHDAMGNVEAF
                     ERILNDER"
     gene            complement(507..1442)
                     /locus_tag="VHR64_10030"
     CDS             complement(507..1442)
                     /locus_tag="VHR64_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3303207.1"
                     /translation="MSNRLTRDQEGLLLGLIGVLAFSFSLPATKEAVPAFGPIMLSLG
                     RAEAAAILAAIVLLIRREPFPGRQHLRSLLLIGAGVVVGFPVLTSYALKHVPSAHGAV
                     VIALLPAATAAFGVLLARERPPRAFWGAVLVGMIAVLIFAIVQGAGKPQWEDALLLGA
                     VVAAAFGYAEGGRLSREIGGWRVISWALIVTAPLLLPFVIADIPNVDWHASTSEWLGL
                     AYVSFVSMYLGFLAWYRGLALGGIARVGQLQLAQPVLTLIWSWLLLSESIGWAEIIAG
                     LVIVGSVALTRRFRYDVGTSRQVAEPAPGLVTIER"
     gene            complement(1457..2248)
                     /locus_tag="VHR64_10035"
     CDS             complement(1457..2248)
                     /locus_tag="VHR64_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX3303208.1"
                     /translation="MFGLRSSRFRSVFVVLVLMAGLLIAALPTDAARAPKVWVIPDNA
                     VPAEYIESIDGDTFDARVMQENGQWREERIRMIGIDTPETNYSYGNHPECYGKEASTR
                     SEGLLLDAREIWLSKDVSDRDRNGRLLRYVWIVSKLDGKVHFLNEDLVKEGYALAKTY
                     KPDTKYQRELDAAERSAIEEARGMWLYCDASVSMDPDQENPDTGPDKTPIDRTKTPVD
                     DEDAVCSFFDTFDEAQDFMELYPEIADVVDKDGDGIACEDYFLGN"
     gene            2449..>2816
                     /locus_tag="VHR64_10040"
     CDS             2449..>2816
                     /locus_tag="VHR64_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303209.1"
                     /translation="MTAEYLTQTPALWRLARGAQTRRTLLRNAAVAGTALFAAPTLLH
                     RSAQAQGLTGKITVGLDGSNTVMVQVVEAAAAAVRAANPQCEIAIEAAPGGNWETQLF
                     LALASGKAPDVFIATGLGVG"
BASE COUNT          524 a          964 c          846 g          482 t
ORIGIN      
        1 gcgttagcgt tcgtcgttga ggatgcgctc gaaggcttcg acgttgccca tcgcgtcatg
       61 gaccgggttg tggtcatggg gcgtgatgcg gaggcgcttc cacgcctggc tattccagaa
      121 ctcgccgacc aagcccgcgt agaagtcgct gattcgccgg gccgagtggc cgaacgggtt
      181 ccggccgagc caatggtgga agccgtaatt gatccactgc cagtcgaagg ccgggttgtc
      241 gctgatgaag atcggccggc ccgtgcagac ctccgccagc cagcgctcga acgcggtgaa
      301 cacgacgacc gggtcgaatc gccggccggt cacctccgac aggtgcgggt tgtcgtctcg
      361 cggagccgat tcgatcagga tgccgtggaa cgtctcccgc gtccggtact cgaccgcgcc
      421 gaactcggtc agctcgccct tgcccgggca cggacccgac gcctcacaat cgacgaagac
      481 caggctgaag cgcggatcat ggatgttcat cgctcgattg ttaccagtcc gggtgctggc
      541 tcagcaacct gtcggctcgt tccgacgtcg taccggaagc ggcgggtcag cgcgaccgac
      601 ccgacgatca ccagcccggc gatgatctcc gcccaaccga tcgattcgct gagtagcagc
      661 cacgaccaga ttagtgtcag gaccggttgc gcgagctgga gctggccgac gcgcgcaatg
      721 ccgcccagcg ccagaccccg gtaccaggcg aggaaaccga ggtacatgct cacgaacgag
      781 acgtatgcga gccccagcca ctcgctggtg ctcgcgtgcc aatcaacgtt ggggatgtcg
      841 gcgatcacga acggcagcag caagggagcc gtgacgatca gcgcccacga gatcacgcgc
      901 cacccgccga tctcccggct cagccgtccg ccctcggcat acccgaacgc cgcagcgaca
      961 accgccccga gaagcagcgc gtcctcccac tgcggtttgc cggcgccctg cacgattgcg
     1021 aagatcagca cggcaatcat gccgaccagc accgcacccc agaacgcgcg cggcggtcgc
     1081 tcccgggcta gcagaacgcc gaatgccgcc gtcgccgcgg gcaacaaggc gatcacgacc
     1141 gccccgtggg ccgacggcac atgcttgagg gcgtacgaag tcaggaccgg aaagccgacc
     1201 actacgccgg cgccgatcag cagaagcgag cggagatgct gtcgtcccgg aaatggctcc
     1261 cgccggatca gcagcacgat cgcggccagg atcgccgcag cctcggcccg gccgagactg
     1321 agcatgatcg ggccgaatgc cggaacggct tccttcgtgg ccggaagcga gaacgagaat
     1381 gcgagcacgc cgatcaggcc gagcagcaag ccctcctgat cccgcgtcaa ccggttgctc
     1441 acgtcacccc cgtcgctcag ttacccagga agtaatcctc gcaggcaatg ccatcgccgt
     1501 ccttgtcgac gacatccgcg atctccggat agagctccat gaagtcctgc gcctcatcga
     1561 acgtatcgaa gaaggagcag accgcgtcct catcgtcgac cggtgtcttc gtgcggtcga
     1621 tcggcgtctt gtccgggccg gtatcggggt tctcctgatc cgggtccatc gagaccgagg
     1681 catcgcaata cagccacatt ccgcgcgcct cttcgatggc ggaccgctcg gctgcgtcga
     1741 gctcgcgctg gtacttcgtg tccggcttgt aggtcttggc gagcgcgtag ccttccttga
     1801 ccaggtcctc attcaggaaa tggaccttgc cgtcgagttt cgagacgatc cagacgtagc
     1861 ggagcagccg cccgttgcga tcccggtcgc tgacgtcctt cgagagccag atctccctgg
     1921 cgtcgagcag cagcccctcg ctgcgggtgc tggcttcctt gccataacac tcagggtgat
     1981 tgccgtagct gtaattcgtt tccggcgtat cgatgccgat catccggatc cgctcttcgc
     2041 gccactgccc gttctcctgc atcacccgcg cgtcgaatgt gtcgccatcg atcgactcaa
     2101 tgtactcggc cggcaccgca ttgtccggaa tcacccagac cttcggcgcc cgcgccgcgt
     2161 ccgtcggcaa tgccgcgata agcaaccccg ccatcagcac cagcacgacg aacacggatc
     2221 gaaaccggga cgaacgcaag ccaaacacca aacgaccaac ctccagtgaa cgccaacctg
     2281 ccctaatcct atcccgccgg aatgggccgc tgcgaatgtt ctggtacgtt tttgcaatcc
     2341 gacattttgc ttcgatgatg tgtgcgtgac ctgccatctg catgatgaag gtggccgcgg
     2401 ccctatcatt ccatctctcc agtagccggg ctgcggggga gtatcaccat gacagctgaa
     2461 tacctgactc agactccagc cctatggcga ttggctcgcg gagcccagac acggcgcacc
     2521 ttgcttcgca acgccgccgt tgctggcact gccttgttcg ccgctccgac cctgctccat
     2581 cgctccgcgc aggcacaggg gctaaccggc aagatcaccg tcggtttgga tggctccaac
     2641 accgtaatgg tccaggttgt cgaagctgcc gccgccgcag tgcgcgcggc gaatccgcaa
     2701 tgcgagatcg caattgaagc cgctccggga ggaaactggg agacccagct cttcctcgcg
     2761 ctcgcgtccg gaaaggctcc cgacgtcttc atcgccacgg gtctcggcgt tggtga
//
LOCUS       DATAOZ010000395         6256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_424203, whole genome shotgun sequence.
ACCESSION   DATAOZ010000395 DATAOZ010000000
VERSION     DATAOZ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424203"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..847)
                     /locus_tag="VHR64_10045"
     CDS             complement(71..847)
                     /locus_tag="VHR64_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3303210.1"
                     /translation="MKLTVLGGSAAGPNTGAGCAGFLITEGDTAIVIDLGPGTLLELR
                     KHVDFRTLDAIVISHYHLDHILDLGAFRYLAMYDPDPLVGKIPLYVPPGTLKRFVKWT
                     AAFGDDEDDSFLEDVFNIAEYDHDQRLEPGSISIRFSRTFHPVPAWAMRISGQNGGEI
                     GYTADTGPSALDSLSEFFLGVSLLISESTEPDDTQDPAEQRGHLTPKEAGKLATNVGA
                     STVILTHRWEELGLDLAATEAGSSFKGHILTARPGVSVFV"
     gene            complement(844..1557)
                     /locus_tag="VHR64_10050"
     CDS             complement(844..1557)
                     /locus_tag="VHR64_10050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX3303211.1"
                     /translation="MSQQERGTFLATNTLPIEVPYSHPTGGRSEFLFVRHGRTEGNVR
                     RILVGRTDIPLDDLGYRQAAAVAEHVGTLARPDVVVASPLLRARETAQAIADVLDLPV
                     EIEPEIAELHFGEYEGWSFEDIRARQPEFAARFADIEFDAHWPGGERLSEFHRRVHGA
                     VTNLATRYASHTAVVVSHGGVLGSLAAQLIGTPPNDWSRYQIQNCSVTHLEISAERSI
                     FHRFNDCAHLDLVNAEPVR"
     gene            complement(1558..3216)
                     /locus_tag="VHR64_10055"
     CDS             complement(1558..3216)
                     /locus_tag="VHR64_10055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX3303212.1"
                     /translation="MKLIFLGGLAEVGRNMFCLESERDIIVVDCGVGFPQEEQLGIDL
                     VLPDISYLREHKDKVRAIFITHGHEDHIGALPYLIPDLAPIPVYATRLTLGLIGAKLE
                     DRKVRSKVDLIELDPDTDTQYTAGDFTVEPFRVCHSIPDAVGFAIDTPAGLIVHTSDF
                     KLDATPIDGRTTDFGKIEKFRKRGVHLLISDCVHIEAKGRTPSEAVIGDTYEEVFAQA
                     EGRIIVATFASLIARIQQIIDVAGRNGRRVATLGRSLEQNVRVALELGFLSDPERVLV
                     DAKEAALMPDHQVVYIVTGSQGEPMAVLSRIANGDHREVSIARGDTVIVSATPIPGNE
                     TAVYRIINKLFRAGADVIYQARALVHVSGHASRDELVDMLRAVDPQFVLPFHGEHRHM
                     ALYADLALEQGIPTDNITFAEVGDVVKVAPEHVSVAGRVDAGFVYVDGLSVGTVGDVV
                     IRDRQALAQDGILMVIVSVDRQTGRIVAGPEIATRGFVHARESGALLDSVKDRLQETL
                     DTYTNGHSTEDWGYLSRQLRDTAANFLFRETRRRPMILPMVMEV"
     gene            complement(3265..3921)
                     /locus_tag="VHR64_10060"
     CDS             complement(3265..3921)
                     /locus_tag="VHR64_10060"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX3303213.1"
                     /translation="MVTEPRTANPDGADSLDSVASEVRVCPLCDLSRTRTLAVPGAGP
                     DTARIMFIGEGPGWHEDQQGLPFVGAAGKFLNELLGLAGLKRGDVFITNVVKCRPPGN
                     RDPLPDEIAACSGYLERQLALIDPLLVVTLGRFSMARWFSGEKISKIHGQARQFGRHM
                     VMPMYHPAAALRSPQVRTQIEADFAKLPSIVEAAEKSRHKLTESHQPEESPLDQLKLF
                     "
     gene            complement(3994..6012)
                     /locus_tag="VHR64_10065"
     CDS             complement(3994..6012)
                     /locus_tag="VHR64_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX3303214.1"
                     /translation="MTDSNLTSEGPNGYDALRNRAFEYLSERNGRVAENELIQFMFGA
                     ASRPELWRTLVQTVLGSDHRFIQIGAQTWALAGNDEVSMGNVVSFVAIDVETTGLRPR
                     QHRMIEIGLARYVNGRCTERFSSLINPERRVPDYIRRLTGLTDSDLIPAPRFGQVAGE
                     IAAFIGNLPLLGHNVGFDMAFLNAEFERAKLPQIVNPTIDTVPMAMRVLGRTVRPSLD
                     RVGTALGLAPRKYHRALADAELAAAVALRLLGMAAEAGVAIESLVTASKNARPIPSGH
                     GQSAGSLLDRRHLDALPRTPGVYLMFDANDRILYVGKAKSIRDRVSSYYSQPLGYTRK
                     MDGLVEAIDRIEHEETGSELVALLLESQLIRRHQPPYNTVLRNSESYPYIRIDPANPW
                     PNLRLAKHRRPDGARYFGPYRSQATAKDAIELLNRRFNLRSCSRGFKTKASYGNPCLE
                     LDLHRCEGPCVGRAMADDYRAGVNAVLGLLDLERDDVLKELEVELAEASEALRFERAQ
                     RLRRELDVLTRLREQQQSLAELVLQEPYLIVQPAPSAALAQLLLIIEGRWWAQVMVEA
                     GAEQHAADRLEAAWKRYLERGMDPIDHSGVDEANIIARWRRSEASAEFVIPVGRAASP
                     DWPILVRHAISTGVIQPGRISGAVDLPFGTFDTLVVASDDVSGSASPS"
     gene            6109..>6256
                     /locus_tag="VHR64_10070"
     CDS             6109..>6256
                     /locus_tag="VHR64_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303215.1"
                     /translation="MVDSGIEHRVAELRAQLDQWNYEYYVLDKPTVSDAEYDEAMNEL
                     RAIEA"
BASE COUNT         1187 a         1913 c         1952 g         1204 t
ORIGIN      
        1 gtacacccgg tccgagcatt tggctcggtc cagtgcccga ccgcaaatgt tgcccggccc
       61 gatgactcgc tcacacgaac accgacacgc ccggcctggc agtcaaaatg tgacctttga
      121 acgaactccc agcctcagta gcggcgagat ccagaccgag ttcttcccag cgatgtgtca
      181 ggatcacggt cgatgcacct acgttggtcg ccaatttccc tgcttctttt ggcgtcaagt
      241 gtccacgctg ctccgcaggg tcctgcgtgt cgtctggctc ggtcgattcg ctgatcagga
      301 gagagactcc caagaagaac tcggagaggc tgtcaagcgc cgatggaccg gtatcggcgg
      361 tgtacccgat ttcccctccg ttttggccag atatccgcat ggcccaggca ggaacagggt
      421 ggaacgtgcg cgagaacctg atcgaaatgc ttcctggttc gaggcgctgg tcgtgatcgt
      481 actcagcgat attgaagacg tcctcgagaa aggagtcgtc ctcgtcgtct ccgaatgccg
      541 ctgtccattt gacgaaccgc tttagtgtcc ctggcgggac atagagcggg atcttcccga
      601 ccagaggatc cgggtcgtac atggccaggt accgaaacgc accgagatcg aggatgtgat
      661 cgaggtggta atgggaaata acgatcgcat ccaacgtccg gaagtcgaca tgtttgcgga
      721 gctcgagcaa cgtgcccggc cccaagtcga tcacgatcgc ggtatcgccc tcggtaataa
      781 ggaatccagc gcagccggca ccggtgttcg gcccggccgc gctgccgccg aggacggtga
      841 gcttcaccga accggctccg cgttgaccag atccaggtgc gcgcagtcgt tgaatcgatg
      901 gaaaattgat cgctcggccg agatctcgag atgcgtcacg ctgcagttct gaatctgata
      961 ccgcgaccag tcattcggcg gcgtgcctat caattgcgcc gccagcgagc cgagcacgcc
     1021 accatgacta acgacgacgg ccgtatgcga ggcatatcgc gtggccaggt tcgtaaccgc
     1081 gccgtggacc cgtcgatgga actcgctgag ccgctcgcca cctggccaat gagcgtcgaa
     1141 ctcgatatcg gcaaaccggg cggcaaactc cggctggcgg gcgcggatgt cctcgaagga
     1201 ccagccttcg tattcgccga aatgcagctc ggcgatctcc ggctcaatct cgaccggcag
     1261 atccagcacg tccgcgattg cctgcgccgt ctccctggcg cgcagcagcg gacttgcgac
     1321 gacgacatcg ggccgagcga gcgtaccgac atgttccgcg actgccgcgg cctggcgata
     1381 accaaggtca tcgagtggga tatcggttcg cccgaccagg atccggcgaa cgttcccctc
     1441 ggtccggccg tgccgtacga agaggaactc gctgcgtccg ccggtcgggt ggctgtaggg
     1501 aacttcgatt ggcaatgtat tcgtggccaa aaacgtgcca cgttcctgct gtgacaatta
     1561 aacctccata accattggca agatcatcgg ccggcgtcgc gtctccctga acaggaagtt
     1621 cgctgcggta tctcttaatt gccggctcag ataaccccaa tcttctgtgc tatgcccgtt
     1681 cgtgtacgtg tcgagcgttt cctgcaagcg atccttcacc gaatcaagca aagcgcccga
     1741 ttcacgtgca tggacgaagc cacgagtcgc aatctcggga ccggctacga tgcggccggt
     1801 ctggcgatcg accgagacga tcaccatcag gatgccatcc tgggcgagcg cctgacggtc
     1861 gcggatgacg acgtcgccga cggtgccgac actcaggccg tccacgtaca cgaagccggc
     1921 atcgacccgt ccagcgacac tgacgtgctc cggtgcgacc ttgacgacgt caccgacttc
     1981 agcgaacgtg atgttgtccg ttggaatgcc ctgctcgagg gccaggtccg cgtagagcgc
     2041 catgtgcctg tgttcgccgt ggaacgggag cacgaactgc ggatcgacgg ctcgcagcat
     2101 gtcgacgagt tcgtcgcggc tggcgtgacc ggagacatgg accagggcac gagcctggta
     2161 gatcacgtcc gcaccggccc ggaagagctt gttgatgatt cggtagaccg ctgtctcgtt
     2221 ccccgggatc ggcgtagctg agacgatcac ggtgtcgccc cgcgcgatcg agacctctcg
     2281 gtgatcgccg ttggcgatgc gggaaaggac agccatcggc tcgccctggc tgccggtgac
     2341 gatgtaaacc acctgatgat ccggcatcaa cgcggcttct ttcgcatcga ccaggacgcg
     2401 ttcaggatcg gacaggaagc cgagctcgag cgctacgcga acgttttgct cgaggctgcg
     2461 gccaagcgtc gcgactcgcc gcccattgcg tccggcgacg tcgatgatct gctggatccg
     2521 ggcgatcagt gaggcgaagg tggcgacaat gatccggccc tcggcctgcg cgaagacctc
     2581 ttcgtaggta tcgccgataa ctgcttccga aggtgtacgg cccttggctt cgatgtggac
     2641 gcagtcggag atcaggaggt gaaccccccg cttccggaac ttctcgatct tgccgaagtc
     2701 ggtcgtccgg ccgtcgatcg gcgtagcgtc gagcttgaag tcgctggtgt ggacgatcaa
     2761 ccccgccggc gtatcgatcg cgaagccgac cgcgtccggg atgctgtgac agacccggaa
     2821 cggctcgacg gtgaaatcgc cggcagtgta ctgcgtgtcg gtgtcaggat cgagctcgat
     2881 gagatctacc ttcgagcgga ccttgcgatc ctcgagcttg gcgccgatca ggcccagcgt
     2941 cagccgggtt gcatagacgg gtatcggggc gaggtccggg atcaggtatg gcagggcgcc
     3001 gatatggtcc tcatggccgt gcgtgatgaa gattgcgcgg accttgtcct tgtgctcgcg
     3061 cagataactg atgtccggca ggacaaggtc gatgccgagc tgttcttcct gtgggaagcc
     3121 gactccgcag tcgacgacga tgatgtcccg ctcggattcc aggcagaaca tgttgcggcc
     3181 gacctcagcg agtccgccga ggaaaatcaa cttcacagat tctaatgtgc taattgtgtt
     3241 gtgcctccag ataggccggg agcgctagaa gagcttgagt tgatctaatg gcgactcttc
     3301 cggctgatgc gattcagtca atttgtgccg tgatttttca gctgcctcga cgatggacgg
     3361 cagctttgca aagtccgcct caatttgcgt gcggacctgc ggtgaacgaa gtgccgccgc
     3421 cgggtgatac atcggcatca ccatgtggcg accgaactgg cgtgcctgtc cgtggatctt
     3481 cgagatcttc tccccggaga accagcgcgc catcgaaaag cgaccgagcg taacgaccag
     3541 gagcgggtca atcagcgcaa gttgccgttc caggtatcca ctacacgcgg cgatctcatc
     3601 cggcaacgga tcccggtttc caggcgggcg acacttgacg acgttcgtaa tgaagacgtc
     3661 accgcgtttc aatcctgcca ggccgaggag ctcgttcagg aacttaccag ccgcgccgac
     3721 aaacggtagg ccttgttgat cctcgtgcca gcccggaccc tcgccgatga acatgattct
     3781 ggcggtgtct ggcccggcgc cggggacggc cagggtgcgc gtacgggaga ggtcgcaaag
     3841 tggacaaacc cggacttcgc ttgcgacgct gtcgagcgag tccgctccgt cggggttcgc
     3901 tgtgcgaggc tccgttacca tgcctcaagt atagccgacc gcctggcgcc accacgttat
     3961 tcaggcgtcc gtcagtccca actggagacg cactcaggat ggtgacgcgc ttccagatac
     4021 gtcgtcgctt gctacaacaa gcgtatcgaa ggttccaaat ggcaaatcga ccgctccgct
     4081 tatacgtcct ggttgaatca ccccggtcga aatcgcgtgc cggacgagaa taggccaatc
     4141 cggcgatgcg gcccggccaa ccgggatcac gaactcggcg ctcgcttccg aacggcgcca
     4201 gcgggcgatg atgttggcct cgtcgacccc ggaatggtcg atcggatcca tcccccgttc
     4261 caggtatcgc ttccaggccg cctcgaggcg atcagcagcg tgctgctccg cgccggcttc
     4321 aaccatcacc tgtgcccacc atcgcccctc gatgatcaac aggagctgtg cgagggccgc
     4381 gctgggcgct ggttggacga tcaggtaagg ctcctggagg acgagctccg cgagcgactg
     4441 ctgctgctcg cgcagcctgg ttaaaacgtc cagctcgcgg cgcagacgtt gtgcccgctc
     4501 gaatctgagc gcctcgctcg cttcggccag ctcgacttcg agctccttca gcacgtcgtc
     4561 tcgctcgagg tccaggaggc cgaggaccgc attcactccg gcgcggtagt cgtcagccat
     4621 cgcgcgacca acgcatggac cttcacagcg atggagatcg agctcgaggc agggattccc
     4681 gtagctggcc ttcgtcttga atccccggct gcacgagcgc aaattgaacc gccggttgag
     4741 cagctcgatc gcatccttcg ccgtcgcctg gctccggtag ggaccgaagt agcgggcacc
     4801 atcgggccgg cggtgcttcg cgaggcgcag attgggccac ggattcgccg gatcgatccg
     4861 gatgtatggg tacgactcgc tattgcgcag tacggtgtta tacggaggct gatgtcgccg
     4921 gatcaattga gattcgagca ggagcgcgac tagctccgat cctgtctcct cgtgctcgat
     4981 acggtcgatc gcctcgacca ggccgtccat tttgcgcgtg tacccgagtg gctgagagta
     5041 gtagctcgag acacggtcgc gaatcgattt cgccttgccg acgtacagaa tgcgatcgtt
     5101 cgcgtcgaac atcagataga cacccggtgt gcgcggcaaa gcatcgaggt gacggcgatc
     5161 gagcagggat cctgccgact gcccgtgacc gctcggaatc ggacgagcgt ttttcgacgc
     5221 cgtaaccaga gactcgattg ctacgcccgc ctcggccgcc atgcccagga gccgaagtgc
     5281 aacggcagcg gcaagctcag cgtcggccaa cgcccggtgg tacttgcgtg gggcgaggcc
     5341 gagagccgtg ccgacccgat cgagcgacgg gcgcaccgtt cgtcccagca cgcgcattgc
     5401 cattggcacc gtatcgatgg ttggattcac aatctgcggc agcttggcgc gctcgaattc
     5461 ggcattcaga aatgccatgt cgaagccgac gttgtgtcca agcaggggta gattgccgat
     5521 gaacgcggca atctcgccgg ctacttgacc gaagcggggc gccgggatca ggtcgctgtc
     5581 agtcagcccg gtgagcctcc ggatgtaatc ggggacgcgc cgttccgggt tgatcagact
     5641 cgaaaatcgc tcggtgcagc ggccgttgac gtacctggcg agtccgatct cgatcatccg
     5701 gtgctggcgc gggcgcagcc cggtcgtctc gacgtcgatc gcgacgaacg agacgacgtt
     5761 ccccatgctg acttcgtcgt ttcccgcgag ggcccatgtc tgggcgccga tctggatgaa
     5821 ccggtggtcg gagccaagga ccgtctggac aagcgtgcgc caaagctccg gccgcgatgc
     5881 cgcgccgaac atgaactgga tcaactcgtt ctcggcgact cgtccgttcc gctccgagag
     5941 gtactcgaac gcgcgattgc gaagcgcgtc gtaaccgttc ggaccctcgg atgtcagatt
     6001 tgaatcggtc aagggaattg tccgtcgcgg gcgaaccaca tgttagaatg cttgttcgta
     6061 tcctacggtg atttcccttt acgctgcaag gcgaacggag tccggtgcat ggtcgattcg
     6121 gggattgagc atcgagtcgc tgagcttcgc gctcagctcg atcagtggaa ttacgagtat
     6181 tacgtcctcg ataagccgac cgtttccgat gctgaatacg acgaagcgat gaacgagctt
     6241 cgagcgatcg aggccg
//
LOCUS       DATAOZ010000396         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6476185, whole genome shotgun sequence.
ACCESSION   DATAOZ010000396 DATAOZ010000000
VERSION     DATAOZ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6476185"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1383
                     /locus_tag="VHR64_10075"
     CDS             166..1383
                     /locus_tag="VHR64_10075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEX3303216.1"
                     /translation="MAEPLPEHPDFLWRNPEPKASYDVVIVGAGGHGLAAAYYLAKNH
                     GLTNIAVLERGWLAGGNMARNTAIIRSNYLWDESSAIFEHSLKLWEGLPEELEYDFLF
                     SQRGVMNLSHTLQDVRDSVRRVNANKLNGIDGDWLDAKQVKELCPIINIGPKIRYPVL
                     GATFQPRGGIAKHDHVAWAFARKADEMGVDLIQNCEVTGFVKDGNRVVGVKTSRGTIA
                     TEKVGLSAAGHSSVLAKLAGFDLPIQSHPLQALVSELHQPVHPTVVMSNHVHVYVSQA
                     HKGELVMGAGIDAYNGYGQRGGFHVIEHQMAAAVELFPIFARAHLLRTWGGIVDVTLD
                     ASPIVSLTPVDNMFVNCGWGTGGFKGTPGAGWTFAHTIATGQAHPLNAPLSLERFVTG
                     ALIDEHGAAGVAH"
     gene            1396..1686
                     /locus_tag="VHR64_10080"
     CDS             1396..1686
                     /locus_tag="VHR64_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX3303217.1"
                     /translation="MILIECPNCGPRDETEYQYGGEAHVPYPEDPHALSDEEWGRYLF
                     YRQNPKGLFAERWVHSAGCRKWFNVLRDTATYRIETVYRLDDPRPNLPGETT"
BASE COUNT          353 a          622 c          549 g          325 t
ORIGIN      
        1 ggctgccgga tcgagcccct cgtgcagatc ctgcgtctcc tgtatcgtca tgatcgccac
       61 ccctcgcgcg ctcgcggttg acattctgtc atccgagaaa gcatactctt attcagcgat
      121 tgatatacta gtcacatact aatcttagtc caggagagcg cagtaatggc ggagccactg
      181 cccgagcacc cggatttcct ttggcgcaac ccagaaccga aggcctccta cgacgtcgtc
      241 atcgtcggcg ccgggggaca tggcctcgcg gccgcttact acttggcgaa aaaccatggc
      301 ctgaccaaca tcgctgtgct cgaacgcggc tggctcgccg gcgggaacat ggcgcgcaat
      361 accgcgatca tccggtccaa ctatttgtgg gacgagtcgt cggcgatctt tgagcactcg
      421 ctgaaactct gggaggggct cccggaggag ctcgagtacg acttcttgtt cagccaacgt
      481 ggcgtgatga acctctccca taccctgcag gacgttcgtg attcggttcg tcgcgtcaac
      541 gccaacaagc tcaacggcat cgacggcgac tggctggatg ccaagcaggt gaaagagctc
      601 tgcccgatca tcaacatcgg ccccaagatc cgctatcccg tgctgggcgc cacgttccag
      661 ccgcgcggcg gcatcgcgaa gcacgaccac gtcgcctggg catttgcccg caaagccgac
      721 gagatgggtg tcgacctcat ccagaactgc gaggtcaccg gtttcgtcaa ggacggcaac
      781 cgggtggtcg gggtcaagac cagtcgagga acgatcgcca ccgaaaaggt gggcctgtct
      841 gcggccggac actcaagcgt gctggccaag ctcgctggct tcgacctgcc gatccagtcc
      901 cacccgttgc aagcacttgt gtccgagttg caccagcctg tgcatcccac ggtggtcatg
      961 tccaatcacg tgcatgtcta cgtgtcgcag gcgcacaagg gcgagctcgt catgggcgcc
     1021 ggcatcgacg cctacaacgg ctatggacag cgcggcggct tccacgtcat cgagcatcag
     1081 atggcggcgg cagtggagtt gttcccgatc ttcgccagag cccatctgct gcgcacctgg
     1141 ggcggcatcg tcgacgtcac gctcgacgcg tccccgatcg tgagcctcac accggtcgac
     1201 aatatgttcg tgaactgcgg ctgggggacc ggcggtttca aaggaacccc aggggccggc
     1261 tggacgttcg cacacaccat cgcaaccggg caggcccacc cgctcaacgc accgttgtcc
     1321 ctcgagcggt ttgtcaccgg ggcactcatc gacgaacacg gcgccgcggg cgttgcgcac
     1381 tgagaggacc tggccgtgat cttgatcgaa tgcccgaact gcggaccccg cgacgaaacc
     1441 gagtaccagt acggcgggga ggcgcacgtg ccctatccgg aggacccgca cgccctcagt
     1501 gacgaagagt ggggtcgcta cctcttctat cgccagaacc cgaaagggct gttcgccgag
     1561 cgctgggtcc actcggccgg gtgccgcaag tggttcaacg tgctccgcga cacagccacc
     1621 taccgcatcg agaccgtcta ccgactcgac gacccacgcc cgaacctgcc cggagaaacc
     1681 acgtgagtac ccagcctcgt cgactacgcc agggcggccg catcgaccgc tcgaccgagc
     1741 tgtccttcac cgtcgacgga cagtcctacg ttggacatcc cggcgacacc cttgcctccg
     1801 ctctgctggc ggcaggcgta atcgagacgg cgccgtctat ctatcgcgc
//
LOCUS       DATAOZ010000397         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6739098, whole genome shotgun sequence.
ACCESSION   DATAOZ010000397 DATAOZ010000000
VERSION     DATAOZ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6739098"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VHR64_10085"
     CDS             <1..568
                     /locus_tag="VHR64_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX3303218.1"
                     /translation="EHYQPPFVAINPNRLVPVLEDGDFRLTESSAILKYLADKFDLPV
                     YPKDLKQRAKVNEIMDWFNTNFYRDYGYGWIYPQLFPHHRRPSEDVQKATVAWGKERA
                     EGWLKVLNDYWLGPDKKYLCGDQITIADYFGISLLTLGEVIGCDFSKYQNVQRWLGNM
                     KKLPSWPRINEALYGFAEAVKGQPFVRL"
     gene            604..1086
                     /locus_tag="VHR64_10090"
     CDS             604..1086
                     /locus_tag="VHR64_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3303219.1"
                     /translation="MIKGLHHNAYRCRDSEETRRFYEDFLGLPLAGALEIKATKSGRA
                     TDTLHTFYALDDGSYLAFFEAPDRPFEFKPQHDFDLHIALEIAECDLHRMFAKGKVAG
                     IETRGISDHGFIDSIYFRDPNGYVIELTAKRANHDQEMDPATNGARRILDAWQAGKAG
                     "
     gene            complement(1323..>1798)
                     /locus_tag="VHR64_10095"
     CDS             complement(1323..>1798)
                     /locus_tag="VHR64_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303220.1"
                     /translation="SSFWMSRWNLEVASSAVFWTGLMQGIGAGSIIGPLGVLTFATLA
                     PAHRTEGASMWNLIRSIGSSIGIATALAILVRTTGINRGVLVEQVTAYNEILRHTTLP
                     VGGGLADPLSLAELDHEITRQAMMIGYVDVFYLCTLTSILAMPLILLLGKPARKR"
BASE COUNT          325 a          596 c          591 g          286 t
ORIGIN      
        1 cgagcactac cagccgccgt tcgtcgcgat caatcccaat cgcctcgtgc cggtgctcga
       61 ggatggcgac tttcgcctca ccgagagctc ggcgatcctc aaatatctcg cggacaagtt
      121 cgacctgccg gtctatccca aggacctgaa gcagcgggcc aaggtcaacg agatcatgga
      181 ctggttcaac accaacttct atcgcgacta tggctacggc tggatttacc cgcagctgtt
      241 tccgcatcat cgccggccga gcgaggacgt ccagaaagcc acggtcgcct ggggcaagga
      301 gcgcgccgaa ggctggctca aggtcttgaa cgactattgg ctcggcccgg acaagaagta
      361 tctgtgcggc gaccagatca cgatcgccga ttacttcggg atcagccttc tgacgctcgg
      421 cgaggtgatc ggctgcgact tttccaagta tcaaaacgtg cagcgctggc tcggcaacat
      481 gaagaagctg ccgagctggc cgcggatcaa cgaggccctc tacggcttcg ccgaggcggt
      541 caagggccag ccgttcgtcc ggctctgagc ggcccggccg aggtcgggcg aggagaggcg
      601 agcatgatca aagggctgca ccacaacgcc taccgctgcc gggattccga ggagacccgc
      661 aggttctacg aggacttcct gggcctgccg ctggccggcg cgctcgagat caaggcgacc
      721 aagagcgggc gggcgaccga cacgctgcac acgttctacg cgctcgacga cggctcctat
      781 ctcgcgttct tcgaggcgcc cgaccggccg ttcgagttca agccgcagca cgatttcgac
      841 ctgcacatcg cgctcgagat cgcggagtgc gacctgcacc ggatgttcgc caagggcaag
      901 gtcgccggca tcgagacgcg cggcatctcg gaccacggct tcatcgattc gatctacttc
      961 cgcgacccga acggctatgt gatcgagctg accgccaaac gcgccaatca cgaccaggag
     1021 atggacccgg cgaccaacgg tgcccgccgg atcctggacg cttggcaggc cggcaaggcc
     1081 ggctgagacc cggcggcgcc gcgcgccggc tccttttggc ctagccggcc gttccggctc
     1141 cttcgtcaac cagccttccg gctccctcgt taaccggccg ttaacgcgtg cccgttaacc
     1201 cttggcacgg cgggtccatg cgaccgtggg actccctgcc ggcgcggagc gcgggaacgt
     1261 ccgctggctc aggctggagg tgggcgacgg gtcgtgccgg cggcttgccg ggatgcgagc
     1321 cgtcaccgct tgcgggcggg cttgccgagc agcaggatca gcggcatcgc caggatcgag
     1381 gtcagcgtgc agaggtagaa gacgtcgaca tagccgatca tcatcgcctg gcgcgtgatc
     1441 tcgtggtcga gctcggcgag cgataacggg tcggcgaggc cgccgccgac cggcaacgtg
     1501 gtatggcgca ggatctcgtt gtaggcggtg acctgctcga ccagcacgcc ccggttgata
     1561 ccggtggtcc gcaccaggat cgcgagcgcg gtcgcgatgc cgatcgagga gccgatcgag
     1621 cggatcaggt tccacatcga agcgccctcg gtgcggtgtg ccggcgccag cgtcgcgaag
     1681 gtcaggacgc ccaagggccc gatgatcgag ccggcgccga tcccctgcat cagcccggtc
     1741 cagaacaccg ccgaggaggc gacctcgagg ttccacctcg acatccagaa gctcgacg
//
LOCUS       DATAOZ010000398         5603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6476353, whole genome shotgun sequence.
ACCESSION   DATAOZ010000398 DATAOZ010000000
VERSION     DATAOZ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6476353"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VHR64_10100"
     CDS             complement(<1..195)
                     /locus_tag="VHR64_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX3303221.1"
                     /translation="MGDALLLGLDIGSTNIKAVVYEPNGAAVSVSNVPQETRYPHAAW
                     AEYDPHRLWDLCCQVIRRAVA"
     gene            complement(197..1039)
                     /locus_tag="VHR64_10105"
     CDS             complement(197..1039)
                     /locus_tag="VHR64_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3303222.1"
                     /translation="MNFGRYLRTALLWLAMTFVVIWVLSPFAWAILTTFKSGTAVYNN
                     QWIPWLQFEPTMRSWQSLFRLPVLKEALATSAVVSIGVASLAVAMAAPASYSIARMKW
                     NARWQTGLLIGFLGQRIMLPVVLITPYMLLSARLGLRDTMQGLIVVNVTFMLPLAVIV
                     IHGAFAGMPPELVEAAHLDGANAFRTFLQIALPLAGPAVVAAWVLCLAFTWNEWLYAD
                     FMTFEKVETMPVALIAVVGGGSGANVPAAMARALSMMVVPIAAALATQRFIASGLSMG
                     AVKA"
     gene            complement(1127..2017)
                     /locus_tag="VHR64_10110"
     CDS             complement(1127..2017)
                     /locus_tag="VHR64_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3303223.1"
                     /translation="MSTAVATMRSRSDERDRATPREIVGKVLLWGAIIAILFWTLFPF
                     YWAIACSIRTNPDSYAIKFLPWIHFDPTLDGWRQMWSLPEIKTALQNSIIIGIGAATV
                     ATILGAFAGYGIARFYFQRPQNGSMTTWFLSQRIIPPVVFVTPFVLFMFQVGLYDNVF
                     GLVLLNATFNLPFPIIILTQMFREVPVELEEAARVDGASRLGIFLKIAVPLVAPGIVV
                     SWILCMAFSWNEFLYAFSTTQDKARPMSVMLVGAEQTRGVDYLFVGTRMVTMMSIPIV
                     AALLVQRYIVRGLSLGAVKG"
     gene            complement(2014..3600)
                     /locus_tag="VHR64_10115"
     CDS             complement(2014..3600)
                     /locus_tag="VHR64_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3303224.1"
                     /translation="MDRAAQIDHGVTGLDPSLALPEDTEEIVRRLLPKAITIGAILLG
                     GALAYDLPALELSDGSTLSINDVNKVLTFSTPSKGWILPLSWFCGLFAILVMIFMPEV
                     ERFARTVGASLGAIGAMALPYYVATNQTVIDKNASGLGSGMISALICFAIAAVVPWIG
                     LLLFTSERPVLGRGWSRWLFVGPAILWILLLTVFPLVYALTTSRYGFRNGRINRAVGW
                     GNYQRAFDEADWGHALETGLRWAIAGIILVVVLGAILSFFSNEYAITVQSIRSIAGFI
                     PVVAIPAFVIGFLTDALADPLDNQLKITVLFVVCVVSVEMVLGVLFALLMNREIRGRA
                     ALRAILTLPLFAAPVGIGYLSRTIFYEGGGPMDRLLGLFGIKPPPWLSDPTWAKISTM
                     IVDIWQWTPFVFVIALAGLQGLPQDIVEAAQVDGASKFQVFRSITLPLMAPILWLILL
                     LRTIDAFKVFDIVASMTLGGPGRSTEYYSFFNYRTARKFFDYGFAAVQAFILLFVVAV
                     IVSVLWNRIKGIYEAEVIQA"
     gene            complement(3714..5324)
                     /locus_tag="VHR64_10120"
     CDS             complement(3714..5324)
                     /locus_tag="VHR64_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3303225.1"
                     /translation="MTDPRIHDLYRMLVENRISRRRFMQAASAMGISATATSFFLQAA
                     NVRAQGATPPPVAQGDGTMFAGQEISVQVIDASVKLPLEEARAEFETATGAKLTIIAD
                     PIENAFSKLIEDAVNGTNAIDGSVIGMWWLGELVEGDYVLSYDDYYNDTSGKFPAFKA
                     EDEFVGMQALRMYDGKRYVLPYDCDGQALYYRRDILTDPTWMDQYKSATGEDLAVPTT
                     WDQMIKISQFFTGKNFNNNPDGGHGVSMHLKVGGQGMFHFMSLSAPYVIGPENKNLYW
                     FDPETMDPLIQSAGHVKAMQAYLDLAKTGPEAQLGWALGEAWDYFLNGNAIFTYSWGD
                     VLPLAIEGDRAVRGKVGTAQLPGTMAYTNPKTGQEYTTTDVNFVGNTTGGSWAPVIMK
                     ATEYPEACYYFFAMLATEPKQRFYAGRGSDGVDPGKVYQMPPEAMDGGTGKIEDYTSQ
                     GFTADEAIEYCKAYYQTFNNPNQLPYLRIPGTADYWSKLDIRLSEAVSGGQAPEDACA
                     NTAKDWNDITEQLGRDAQLASYKRSLGL"
BASE COUNT         1185 a         1753 c         1656 g         1009 t
ORIGIN      
        1 ggcaaccgcc cggcggatca cctggcagca gaggtcccag aggcgatgcg ggtcgtactc
       61 ggcccaggcc gcgtgcgggt aacgcgtctc ctgcggcaca ttggaaaccg acaccgcggc
      121 gccattcggc tcgtagacca ccgccttgat attcgtcgac ccgatatcca acccgagcag
      181 cagcgcgtca cccatatcag gcctttaccg cacccatcga aagtccgctg gcaatgaacc
      241 gctgcgtcgc cagcgcggcc gcaatcggaa ctaccatcat cgagagtgcc cgggccatcg
      301 ccgccggcac atttgcaccg cttccgccgc ctaccactgc aatgagcgcg accggcattg
      361 tctctacctt ctcgaacgtc atgaaatcag catagagcca ctcgttccag gtgaaagcca
      421 ggcaaaggac ccaggcggca acgactgccg gacccgccag cggcagggcg atttgaagaa
      481 atgttcgaaa ggcgtttgcg ccatcgaggt gagccgcctc taccagctcc ggcggcatac
      541 cggcaaaggc gccgtggatc acgatcaccg ctagtggcag catgaaggtg acattgacga
      601 ctatcagccc ctgcatggtg tccctgaggc caaggcgggc ggagagcagc atatagggag
      661 tgatcaacac gaccggcagc atgatccgct gtccgaggaa cccgatgagc aagccggtct
      721 gccagcgggc attccacttc atccgggcga tgctgtacga cgccggagca gccatcgcca
      781 ccgcaagcga cgccaccccg atggagacaa ccgccgaagt cgccaacgct tccttcaaga
      841 ccgggagccg gaaaagcgat tgccagctgc gcatcgtcgg ctcaaactga agccatggaa
      901 tccactggtt gttatagaca gccgtgcccg acttgaacgt cgtcaaaatc gcccaggcaa
      961 acggtgaaag cacccatatc actacgaatg tcatcgccaa ccaaagcagt gccgtccgca
     1021 gatatcgacc gaaattcacc ggcggttcct gatagcgaag ggaacgggcg tagccgcccg
     1081 ttcccaatca atcgcgttcg tgctcgactc ccgtaacgtg ccttggctac cccttgaccg
     1141 cgccaaggga gagtccgcgc acgatgtacc gctggacgag gagggcagcc acgattggga
     1201 tcgacatcat cgtgaccatg cgcgtgccca cgaagaggta gtccactccg cgagtctgtt
     1261 cggcgccgac cagcatgacc gacattggtc gcgccttgtc ctgcgtcgtc gagaatgcgt
     1321 acaggaactc gttccagctg aatgccatgc acagaatcca gctcacgacg attcccggcg
     1381 cgacgagtgg cactgcgatc ttcaggaaga tcccgagtcg agatgcgcca tcgaccctcg
     1441 ccgcctcctc gagctcaacc ggcacctccc ggaacatctg tgtcaggatg atgatcggaa
     1501 acggcagatt gaaggtcgcg ttcaggagca ccaggccgaa cacgttgtca tacaggccaa
     1561 cctgaaacat gaacaacacg aacggtgtga cgaaaaccac gggcggaata atccgttgcg
     1621 acaggaacca ggtggtcatg ctgccgttct gcggtcgctg gaagtagaag cgtgcaattc
     1681 cataaccggc gaaggcgcca agaatcgtcg cgaccgtcgc agcgccaata ccgataatga
     1741 tgctgttttg cagggctgtc ttgatctccg gcaggctcca catctgtcgc cagccgtcga
     1801 gggtcggatc aaagtgaatc cacggcagaa acttgattgc gtagctgtcg ggattggtcc
     1861 tgatcgagca ggcgatcgcc cagtagaacg ggaagagcgt ccagaacagg atcgcgatga
     1921 tcgcgcccca gagcagcact ttcccgacga tttctcgcgg ggtcgcccga tcgcgctcgt
     1981 cggatcggga acgcatggtt gccactgcgg tgctcatgcc tggatgacct ccgcttcgta
     2041 gatgcccttg atccggttcc acaggacaga aacgatgacc gcaacgacaa acagcagaat
     2101 gaaggcctgc accgccgcaa acccgtaatc aaagaatttc cgggcagtgc ggtaattgaa
     2161 gaagctgtaa tactcggtcg atctaccagg accgccaagt gtcatcgagg caacgatgtc
     2221 gaaaaccttg aaggcgtcga tcgtgcgcag gagcaggatc agccagagga tcggcgccat
     2281 cagcggcaac gtgatcgatc ggaagacctg gaatttgctg gcgccatcga cctgtgccgc
     2341 ctcgacaatg tcctggggca acccctgcag accagccaac gcgatgacga agacgaacgg
     2401 cgtccactgc cagatgtcga caatcatggt cgagatcttg gcccaggttg ggtcggacag
     2461 ccacggcgga ggtttgatcc cgaataagcc aagcaagcgg tccatcgggc cgccgccctc
     2521 atagaagatc gtgcgagaga gatacccgat cccgaccggc gccgcgaaca acggaagcgt
     2581 caggattgcg cgcaaagccg ctcgtccgcg aatctcgcga ttcattagca gtgcgaagag
     2641 tacgcccagc accatctcga ccgaaaccac gcacacgaca aacagcacgg tgatcttgag
     2701 ctgattgtcg agcgggtcgg ccaatgcgtc ggtcaggaag ccgatcacga aggcaggaat
     2761 cgccacgacc gggataaacc cggcaattga gcgaatcgac tgcaccgtaa tcgcatactc
     2821 gttggaaaag aaggacagga tcgcgccaag aacgaccacc aggatgatgc cagcgatagc
     2881 ccaccgcaga ccggtctcga gcgcgtgtcc ccagtcggcc tcgtcaaatg ccctctgata
     2941 gttaccccag ccgactgcgc gattgattcg gccgttccgg aatccatagc ggctggtcgt
     3001 caatgcatag acgagtggga agacggtcag gagcagaatc cagaggatgg ccggaccgac
     3061 gaacagccat cgtgaccagc cccgtccgag gactggtcgt tctgacgtaa acagcaggag
     3121 tccgatccac ggcacgacgg cggcgatcgc gaagcagatc agcgcggaaa tcatcccgga
     3181 gccaagtccg gatgcgttct tgtcgataac ggtctggttc gtcgccacgt agtacggcaa
     3241 cgccatagcg ccgattgcac caagcgatgc cccgacagtc cgcgcaaatc gctcgacttc
     3301 cggcatgaag atcatgacca ggatcgcgaa gagtccgcag aaccaactca aggggagaat
     3361 ccaccctttg cttggtgtgg aaaacgtgag gaccttgttg acgtcgttga tcgaaagagt
     3421 cgaaccgtcg ctcaactcaa gtgccggcaa gtcgtaggcc agcgcaccac cgagcaaaat
     3481 cgcgccgatc gtgatcgcct ttggcagcaa tcgccggacg atttcttcgg tgtcttcagg
     3541 cagcgcaagg ctcgggtcca gccccgttac cccatgatcg atttgtgcgg ctctgtccac
     3601 ctgcgccatc gcggtacccc cgattatcgc cctgccggtt ctgcgcgcgt atggaatggt
     3661 tggggatctc ggtggcccgg ccgggccacc gagatgtgta gtctaccgac ccgctagagg
     3721 ccgagcgacc gcttgtagga cgcgagctgg gcatcgcggc cgagctgctc ggtgatgtcg
     3781 ttccagtcct tggcggtatt ggcgcaggca tcttccggcg cttgaccacc tgagacagcc
     3841 tcggagagcc ggatatccag cttgctccag tagtccgccg tgcccggaat gcgcaggtac
     3901 ggaagctggt tcgggttgtt gaaggtctga tagtacgcct tgcagtattc gatcgcctcg
     3961 tcggcggtga acccctgcga ggtgtaatcc tcgatcttgc ccgttccgcc gtccatggct
     4021 tccggcggca tctggtagac cttgcccggg tcgacaccgt cggaacctcg accggcgtag
     4081 aagcgctgct tcggctcagt cgcgagcatc gcgaagaagt agtagcaggc ctctgggtac
     4141 tcggtcgcct tcatgatcac cggcgcccac gagccacctg tcgtgttgcc gacgaagttg
     4201 acgtcggtgg tggtgtactc ctgacccgtc ttcgggttgg tgtaggccat cgtgcctggc
     4261 agctgagccg ttccgacctt gccccgaacc gcgcggtcgc cttcgatcgc cagtgggagt
     4321 acatcgcccc agctataggt gaagatggca ttgccgttca ggaagtagtc ccacgcctcg
     4381 ccgagcgccc agccgagctg cgcctccggt ccggtcttgg ccagatccag gtacgcctgc
     4441 atcgccttga cgtgtcccgc cgactggatc agcggatcca tagtttccgg atcgaaccag
     4501 tagaggttct tgttctccgg cccgatcacg tacggggccg acagcgacat gaagtggaac
     4561 ataccctgac cgccgacctt gaggtgcatc gaaacaccgt ggccgccgtc cgggttgttg
     4621 ttgaagttct tgccggtgaa gaactgggag atcttgatca tctgatccca ggtcgtcgga
     4681 acagcaagat cttctccggt tgcggatttg tactgatcca tccaggttgg atcggtcagg
     4741 atatcccgcc ggtaatagag tgcctgccca tcgcagtcgt acgggagaac ataccgcttc
     4801 ccgtcgtaca tgcgcagcgc ctgcataccg acgaattcgt cctccgcctt gaatgcgggg
     4861 aacttgcccg acgtatcgtt gtagtagtca tcatacgaga gcacgtaatc gccctcgacg
     4921 agctcaccca accaccacat gccgatgacc gagccgtcga tggcattggt cccgttgacg
     4981 gcatcctcga tcagtttcga gaacgcattc tcgatcgggt cggcgatgat ggtcagcttg
     5041 gcaccggtcg ccgtctcgaa ctctgcgcgc gcctcttcga gcgggagctt gaccgaggcg
     5101 tcgatcacct gcaccgagat ctcttgcccg gcgaacatcg tgccatcgcc ttgcgccacc
     5161 ggaggcgggg tcgcgccctg cgcccgtaca ttggctgcct gcaagaagaa cgacgtggcc
     5221 gtcgcggaga tccccattgc cgacgccgct tgcataaagc gccgccggga gattcggttc
     5281 tccaccaaca tccgatacag gtcgtgaata cgtggatctg tcaccgcaca gtctccctca
     5341 cgatatgaac ctgctctccg gcacctgcgc cggggccctc gccggccaaa cgtcgaccgg
     5401 aatcaagagc cggagccgag ctcccatatg ttggttcgcg gcagactccc atgcctgccg
     5461 ccggatcggc cggtcggccg gttacgaccc gtggatgatg tcgcgcaggc ggcgcgagac
     5521 ggcgaccgtg tcgtcctgct gccagacgtt gcggccgtag atgacgccct tggcgccgac
     5581 atcgatcgcg ttcctggtgc ctt
//
LOCUS       DATAOZ010000399        13305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8844833, whole genome shotgun sequence.
ACCESSION   DATAOZ010000399 DATAOZ010000000
VERSION     DATAOZ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8844833"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VHR64_10125"
     CDS             <1..433
                     /locus_tag="VHR64_10125"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3303226.1"
                     /translation="TIRLGPLGIRFLVTGADSRGSAAVFECSVPAGEQLRAPAHSHDA
                     YEETIYGLEGVLTWTVDGQPIGVAPGQALCIPRGAVHRFDNFGQEDAKVLIIASPAKI
                     GPEYFHEVAAAMAATAGGHPDPSRMADVMRRHGLTPVAPGA"
     gene            complement(507..662)
                     /locus_tag="VHR64_10130"
     CDS             complement(507..662)
                     /locus_tag="VHR64_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303227.1"
                     /translation="MEHALSTLMLVWGLIGLVTLWVAANALADFGARRLRHWYHTTRR
                     HPHSGED"
     gene            896..1825
                     /locus_tag="VHR64_10135"
     CDS             896..1825
                     /locus_tag="VHR64_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX3303228.1"
                     /translation="MARSQNQCPVCKAEAGPGYRYCLACGAELNPDAAYVEPQPEPEA
                     VAEPKPDKKKWGRKKQAEPVAPAPSSPEAYSYSYASFAAIEGPPEVNPRAKKRSGGRL
                     RGFLLFLILVGGAVGGMMYWNEHPTIAEGRIPGRISWEAVRDGDFGNAWISTEESTKQ
                     ELLAQVPSGAVEATVTSVGTDGLVTIQLRSRDVPVRLAGVPVDFSAQCLGDKGIGRVG
                     RALPEGALVYVLLDGEGRLSAKADAPQPVYIWRMDIEGKKLRFVNQELIASGEAEYVP
                     VTLSDQDPGASLGRAGQRAQDKQRGRYAEGACI"
     gene            1893..3122
                     /locus_tag="VHR64_10140"
     CDS             1893..3122
                     /locus_tag="VHR64_10140"
                     /inference="COORDINATES: protein motif:HMM:NF006769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEX3303229.1"
                     /translation="MNQPLTVNADRLHASLADLAQIGATPAGGVTRLALSDEDRQARD
                     LLSTWLRDAGLEPKVDDVGNISAIRPGRESAPPVLLGSHIDTVVRGGRYDGALGVLGA
                     LEVIRTLDDHGIETRLPIGLVNWTNEEGVRFEPAMTCSGVVTGRFTPEYVGERTDRAG
                     LRFADELARIGYQGDAANRSLPASAYLELHIEQGPVLESQNLPAGVVGGIVGITWNEV
                     TIAGQADHAGPSPMGLRRDALTAAAEIILGVEQIAKRRDETAVATVGRIAAQPNVINT
                     IPGQVVFSVDFRHSDPAILEQQVAALHQLVDEVATRRSVEATIERFWTSEPTPFDTRV
                     QDAIRLSAAALGVSTGELWSGAGHDAKYLADVCPTGMIFVRSQGGLSHCEAEYSAPAD
                     IEAGANLLLNAAINLAG"
     gene            3127..4509
                     /locus_tag="VHR64_10145"
     CDS             3127..4509
                     /locus_tag="VHR64_10145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3303230.1"
                     /translation="MTDYRAISYWLETAGDDLTPRPSLPESIDVDVAVLGAGFSGLWT
                     AWYLKERDPSLEIAIIEREIAGFGASGRNGAWCTSEFPTGPGALAHKYGDDTARTVVT
                     AMRETVAEIKRVTEAEGIACDWADGGQLIIARGPQQRPAVDDEYQTLRRLGLADDLEL
                     LAADQARQRLNVTELQSALFSPHTAVIHPGKLVRGLARAVERRGVKIYEQTAVTKVHP
                     GRYPTLETTQGNVRAKAIVLCGEAYLSQLPGYGRAVMPVYSLITLTEPLSDADWAEIG
                     WRNRECVASCRYTVDYLSRTADGRILFGGRGAPYRFGSKITPEMDKHAGTHQMLQDNV
                     RAWFPRLKDIRFTHTWGGPLGWSRDFMPTVSFDPAQGLGMAHGYTGNGVSTTNLTGRM
                     LADMITGQESALLELPHANHKTRQWEPEPFRYLGVRFVQQSFMRIDRKSEETGLPPNG
                     KSLAERLTAH"
     gene            4588..5145
                     /locus_tag="VHR64_10150"
     CDS             4588..5145
                     /locus_tag="VHR64_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX3303231.1"
                     /translation="MRSLRYSINVTLDGCCDHRAGSTDDELHRYWAESLAQADALLFG
                     RVTYEMMEAAWRLPATGVLPDWMADWMEPFARTIDAAKKYVVSSTLDQVDWNAELVRG
                     DLGEAVQQLKAEPGASLFVGGLTLPLTLAELGLIDEYEFVVQPRIAGHGPTLFAGLSK
                     YLDLELVSRREFRSGAVALRYVPRR"
     gene            complement(5203..6354)
                     /locus_tag="VHR64_10155"
     CDS             complement(5203..6354)
                     /locus_tag="VHR64_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX3303232.1"
                     /translation="MTERPSNLGIARSGPTPERWRPLSPWGLFLTLLLFYVLFKVRLV
                     VEIGLLAFLYATVIERPVRRLGRHGVPRAASIILVDLAIVAGLVLPILAIAPAASREV
                     DRFRAEEPDRLRTLDQQWATSGNPLLSGPGRDIVERAIVELEAPPSEAPPKAVLSALG
                     TTALWIIAGLACLVMAYFYMLEKDLLRMMLLENVPPRSRLRIAGLWDAVEESIGGWLR
                     SRLILGVIVGIVSMIVFGLMGLPYWPLLGLVAGLTEPIPIIGPWIGGVPAVFLALTVS
                     PLLAVAVVGFILARQMVVDAVIVPRVTKEAVGLSPLTVFAAVLAGTELLGPEGALLAI
                     PVAAAVQIVVVDALAVRRGYESRPTLRWRWLSKQDAAIQDSGRDGSAGN"
     gene            complement(6403..7689)
                     /locus_tag="VHR64_10160"
     CDS             complement(6403..7689)
                     /locus_tag="VHR64_10160"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303233.1"
                     /translation="MRVMLTRIARRPAGELWRSPAFLRLWAASAVSAAGDQVTRLALP
                     IIAVTTLGAGPFHVGLLLTFEQLPQLLFTLVAGAWIDRLPKRPVIMICDAGRALVLLA
                     VPIAAWLDMLSMPGLYAIGFLAGVFTTWHMIAWQSLLPMITPGSEMIAGASAITQVEA
                     MAEVAGPVAGGGLVQLVGAPAAVLIDAVSFAGSAALIGQITVEEPPATAVQTSILSEI
                     RAGFRYLFSDPILRAIGLSGGLGVFFYSIREPLLRVFLLDTKGLSAGAYGLVFTVAAI
                     GFAIGAFLPGPVARRVGVGNAIVWPNVGLTLTAIVIAAAVMLDWHAVAVISGMLFLEG
                     VIEPTNNINQLSLRLALMPREMRGRLTSVVRFVIRGAYPLGTLVGGAVGQWIGIGNAI
                     WLTVLCGPLGMAAYIGTSILQYRQLPAVEVEGSAEA"
     gene            7955..9916
                     /locus_tag="VHR64_10165"
     CDS             7955..9916
                     /locus_tag="VHR64_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3303234.1"
                     /translation="MMSRISTRVRFILAMMLLLVPIVAIAAIILDESFKRSQEQIIIS
                     EFATADVVSQSVAELIRGQKEALSVLAEEQSVRSLDQGKEQALALMDDYRVSRQPVNG
                     VFLLKNDLTVVAQSGGIDISSLPPEFNDAAKAAVDGGQVTVSDSLLLSNGEVKVIAII
                     VPVYASTEASSTEMPSGAIGAFYSVERLIAGFQPPAGFTTGSNVAIALVSSTGRIISV
                     PGRTSDPSQIFQDGPALNNAIKSALASQRTRTTFQDPTGLHRVTVGVPIDMPGNDWAV
                     LVSGPETTAFGPNKMLIERALLALIGSVVICFLLALLFSEWLTRPFRLLTSQAKALST
                     GAVVLDLEPVGPSDAAILSRTIREMADRLRAQIRDTEAAREEIARQAERLRDLLRRTV
                     RLQEDERRRIATDIHDAVSPLITGALYQAQAVRIARSGSGNGNGNGHDGVNGHHPDQE
                     EANEGLQEVGELLERAMRELHDVIFDLRPPDLDDIGLEAAIQRHVDQVNRTGLRCSLE
                     VVGEERRLSPEARLAIYRIVQEALHNAIRHARADESLVRIEWLADRLRVTVQDDGSGF
                     ETEGSGWRAGLGLMSMRERAGSIGAAFEVVSRPGTGTAIVLERMNDGLDLTAEHPVLD
                     PELAPSQSPADGDIVDEDNVDVGEEHLVQ"
     gene            9913..10548
                     /locus_tag="VHR64_10170"
     CDS             9913..10548
                     /locus_tag="VHR64_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3303235.1"
                     /translation="MISVLLVDDHPVVVEGLRKLLTQTGEVVIAAEANDAATGIKLAK
                     QIQPDVVLLDLRMPGATGIQAVRRMREQEVRSAVIILTSYGDQAYVRQAIEAGADGYL
                     LKSTPPDDLIQSIRNAARGRKPLSPELIDGVLTDFSGLAREQTRRNADLTDDDREILR
                     RASEGATNKEIGFAMGRSEIAIKKRLQVIFSKLGAVDRAHAVAESIRRGLI"
     gene            complement(10788..11933)
                     /locus_tag="VHR64_10175"
     CDS             complement(10788..11933)
                     /locus_tag="VHR64_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX3303236.1"
                     /translation="MNRRERWIMTVSLTIGMLLMLVLAGTGAIDFGGAMAQDTPSTVA
                     RLQEASPTAESSTGSGQMSSAVDVVKQVSPAVVTVVNEQRVGMQNGGSSLQPVGSGTG
                     FIIDNDGHIVTNWHVVNGGSKFQVVFSDGTTKGAKLIGSDEISDLAVVQVDGDMPGTI
                     ALGDSDAVQVGESVLAIGSPLGNFQNTVTEGIVSATNRDFPGDGSTQSQYNNLIQHDA
                     AINPGNSGGPLIDFNGEVIGVNTLGISVDNQGQPIQGLFFAIPSNSVKKVTDELIKSG
                     KVTYPYFGIAYVSNSAAAAAQLGLSTDQGVIVQDVPPSGPAADAGIQQGDVITKVGDY
                     ELNAQTTFSEALFNYKPGDTIDVVVKRGDKDMTVSVTLGDRSKSLSQ"
     gene            12071..12823
                     /locus_tag="VHR64_10180"
     CDS             12071..12823
                     /locus_tag="VHR64_10180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX3303237.1"
                     /translation="MSTAATDLLRDFEALFARTEVVDLSAVLEEGAPIWPTHPPLIIH
                     RTVTHERHGYFTNSIFMPEHVGTHCDAPAHAIPELMDQTIDRFPIDRMMGPACVIDVS
                     ARNLEAGEMLTAQDILDWEAEHGQIRPGDIVLINFGWFARHWRTDEQARYYVTHQPGL
                     DEAALKLIYERGATALGSDNTNTETPIRDGKSLTPSYAHTTYFLPNGIPLIESLANLE
                     RLPPHCYFIALPLKIKGGSGSPIRPIALIPEG"
BASE COUNT         2369 a         4679 c         4086 g         2171 t
ORIGIN      
        1 gacgatccgc ctcgggccgc tcgggatccg tttcctcgtc acgggggcgg actcccgcgg
       61 aagcgctgcc gtgttcgagt gctcggttcc cgccggcgag caactccggg cacccgcgca
      121 tagccacgac gcctatgaag agaccattta cggcctcgag ggcgtgctca cctggacagt
      181 cgatggccaa ccgatcggag ttgctccggg gcaagcgctc tgcattcccc gtggcgcggt
      241 ccatcgcttc gacaatttcg gccaggagga tgcgaaggtc ctcatcatcg cctcgccggc
      301 gaagatcggg ccggaatatt tccatgaagt ggccgcggca atggcggcta ccgctggtgg
      361 acatcctgat ccgagccgga tggcggacgt gatgcgccgt catggcttga ctccggttgc
      421 gcccggcgcc tgaacaggtg cctgctggcc tcgccagagt gggccacgtg cgaatccagg
      481 aatgtgcggc gaccagtact ccggtgctaa tcttcgcctg aatgtggatg gcggcgcgtg
      541 gtgtgatacc agtggcggag tctgcgggcg ccgaaatcag ccagcgcgtt ggcggcgacc
      601 cagagcgtga ccagcccgat cagtccccag accaacatca gcgtcgaaag ggcgtgctcc
      661 atgcctgaac gacccccggt tcgattgtcg taccgcgact gatcgcactg tacgtacacc
      721 cgtgcaaact gtcaataccc gccgctcggc atgaccgcaa tgggcagagc cgctccggca
      781 acttggcacc tccgggggat acggtgccgc gcatatctgc ccaaccatac gtttgggatt
      841 gacccgaccg tgcgcgctcg cccgcgagtg ccgcccgtgg tgcggaggag tgacgatggc
      901 tcgatcacag aaccaatgtc cagtctgcaa ggccgaggct ggaccgggat accgatattg
      961 cctggcgtgc ggcgcggagc tgaatcccga cgccgcatat gtcgagcctc agcccgagcc
     1021 ggaggctgtg gccgagccaa agccggacaa gaagaagtgg ggtcggaaga agcaggctga
     1081 acctgttgcg ccggcgccct cgtcgccaga ggcctattcc tattcatatg cctctttcgc
     1141 ggccatcgaa ggaccgcccg aggtcaatcc gcgcgcgaag aagcgctccg gcgggcgcct
     1201 gcgcggcttc ctgctcttcc tgatccttgt cggaggagcg gtcggcggca tgatgtactg
     1261 gaatgagcac ccaacgatcg ccgagggccg catccccggt cgcatctcct gggaggcggt
     1321 tcgcgacggc gacttcggca atgcctggat cagcaccgag gagtcgacga agcaggagct
     1381 gctggcccag gtgccgtccg gcgcggtcga ggccacggtc acatcggtcg gcaccgacgg
     1441 gctcgtgacc atccagcttc gctcgcgcga tgtcccggtc cgactagccg gcgtcccggt
     1501 cgatttctcc gcccagtgcc tcggcgacaa gggcatcggt cgcgttggtc gggcgttgcc
     1561 ggaaggcgca ctcgtctacg ttttgctcga tggcgagggc cgcctgagcg cgaaggcgga
     1621 cgctccacag ccggtctaca tctggcgcat ggatatcgag ggcaagaagc tccgcttcgt
     1681 caaccaggag ctgattgcga gcggcgaggc cgagtacgtg cctgtcactc tcagcgacca
     1741 ggacccgggg gccagcctcg gtcgtgccgg gcagcgcgcg caggacaagc agcgcggcag
     1801 gtatgccgag ggcgcctgta tctgagcgat gcggctgaac gtatgattcc gccggggccg
     1861 ggtgtcgttc cgcccgcgcc ggcgggattg ctgtgaatca acctctgacc gtcaacgcgg
     1921 accggctcca tgccagcctc gcagacctcg cccaaatcgg cgcgaccccg gccggcggcg
     1981 tcacgcggct tgccctctcg gacgaggacc gccaggcccg cgatctcctg tccacctggt
     2041 tgcgcgacgc cggtctcgag ccgaaggtcg acgatgtcgg caacatctcc gcgattcggc
     2101 ccggccggga gtccgccccg cccgtcctgc tcggctcgca cattgacacc gtcgtgcgcg
     2161 gcgggcgata cgacggcgcg ctcggggtgc tcggcgcgct cgaggtcatc cgcacgctcg
     2221 atgatcacgg tattgagacc cgcctgccga tcggtcttgt caactggacc aatgaagaag
     2281 gcgtccgctt cgaaccggcc atgacctgct ccggcgtcgt caccggccgt ttcacgcccg
     2341 aatacgtcgg cgagcggacc gatcgggctg gtctgcgctt cgccgatgag ctggcccgga
     2401 tcggctacca gggcgacgcc gccaatcgtt cgctgccggc ttcagcctac ctcgagctgc
     2461 acatcgagca ggggccggtt ctggaatcgc agaacctccc ggccggtgtc gtcggcggga
     2521 tcgtcggcat cacctggaat gaagtcacga tcgccggtca ggccgaccac gccggcccga
     2581 gcccgatggg ccttcgccgg gatgcgctga cggccgccgc tgagatcatc ctcggtgtcg
     2641 agcagatcgc gaagcggcgg gacgagactg cggtggcgac ggtcggtcgc atcgccgcgc
     2701 agccgaacgt gatcaacacg atccccggtc aggtcgtatt tagcgtcgac ttccgccact
     2761 cggatccggc catcctcgag cagcaggtcg cggcgctcca ccaactggtc gacgaggttg
     2821 caacccgccg ctcggtcgag gccacgatcg agcgcttctg gactagcgag ccgaccccgt
     2881 tcgatacccg ggtccaggac gcgatccggc tatccgccgc tgccctcggg gtctcgaccg
     2941 gcgagctctg gtccggcgcc gggcacgacg ccaagtacct ggccgatgtc tgcccgactg
     3001 gcatgatctt cgtccgcagc cagggcggac tcagtcactg cgaagccgaa tacagcgcac
     3061 cggccgacat cgaagctggc gccaacctgc tcctcaacgc cgccatcaac ctcgcagggt
     3121 agggaaatga ccgattaccg ggccatctcg tactggctcg aaacggcagg tgacgacctg
     3181 acgccgcgcc cgtcgctccc cgaatcgatc gacgtcgacg tcgcggttct cggggccggt
     3241 ttttccggtc tctggaccgc ctggtacctg aaggagcgcg acccctcgct cgagatcgcg
     3301 atcatcgagc gggagatcgc cggcttcggc gccagcggcc gcaacggcgc ctggtgcacc
     3361 agcgagttcc cgaccggtcc cggcgcgctg gcccacaagt acggcgacga cactgcccgg
     3421 actgtcgtca cagccatgcg ggagacggtc gccgagatca agcgggtgac cgaagccgaa
     3481 gggatcgcct gcgattgggc cgatggcggc cagttgatca tcgctcgcgg cccgcagcaa
     3541 cgcccggccg tcgatgacga gtaccagaca ctgcggcggc tcggcctggc cgacgacctc
     3601 gagctgcttg ctgccgatca ggcccggcag cggctcaatg tgaccgaact gcagagcgcg
     3661 ctcttctcgc cccacaccgc ggtcatccat cccggcaagc tcgtccgggg cctggccaga
     3721 gccgtcgagc ggcgcggcgt caagatctac gagcagacgg ccgtaacgaa ggtccatccc
     3781 ggccgctacc cgacgctcga gaccacccag ggcaacgtcc gggccaaagc gatcgtcctc
     3841 tgcggtgagg cctatctgag ccagctcccc ggctacggcc gcgccgtcat gccggtctac
     3901 tccctgatta cgctgaccga gccgctctcc gatgccgact gggccgagat cggctggcgg
     3961 aatcgggaat gcgtcgcgtc gtgccgctac acggtcgact acctctcccg gaccgccgat
     4021 ggccggatcc tcttcggcgg acgtggcgcc ccataccgct tcggctcgaa gatcacgccc
     4081 gaaatggaca agcacgccgg gacgcaccag atgctgcaag acaacgtccg tgcgtggttc
     4141 ccgcggctca aggacatccg cttcacccac acctggggcg gtccgctcgg ctggtcgcgc
     4201 gatttcatgc cgactgtctc gttcgacccc gcgcagggcc tcggcatggc ccacggctac
     4261 accggcaacg gcgtctcgac gaccaacctc actggccgga tgctggccga catgattact
     4321 ggtcaggaat cggccctgct cgagctgccg cacgccaatc acaagacccg ccaatgggaa
     4381 cccgagccct tccgctatct cggcgtccgc ttcgtccagc aatccttcat gcgcatcgat
     4441 cgcaaatcgg aggagaccgg cctcccgccc aacggcaaat ccctcgccga gcgcctcacc
     4501 gcgcactaga gccgatgccc acggattgcc cgtcaccgtt tccgggaacg cgcccatcct
     4561 cgccgtgacc gatggtacga tgccgccatg cgatcccttc ggtattccat caacgtcacc
     4621 ctggacgggt gctgcgatca tcgcgccggt tcaacggacg acgagctgca tcggtactgg
     4681 gccgagagtc tcgcgcaggc cgatgccctt ctctttggcc gggtaaccta cgaaatgatg
     4741 gaggccgcat ggaggctgcc ggcgaccgga gtgctgccgg attggatggc cgactggatg
     4801 gaaccattcg cccggacgat cgacgcggcc aagaagtacg tcgtgtcgag caccctggac
     4861 caggtcgatt ggaacgcgga gctcgtgcgc ggggatctgg gggaggccgt tcagcagctc
     4921 aaggcggagc cgggggcgag tctcttcgtg ggcggcctga cgctcccgct gacgttggcg
     4981 gagctgggct tgatcgatga atacgagttc gtggtgcagc ccagaatagc gggccacgga
     5041 ccgacactat tcgctgggct ctcgaaatat cttgacctgg agctcgtgag ccggcgggag
     5101 ttccgctcgg gggcggtcgc gcttcgatat gtgccgagac ggtagccctc gggcgtggtc
     5161 gcgggggtct gccagcgatc tggcgttggc acaacgtgac gttcaattcc cggcggagcc
     5221 gtcgcgcccg ctgtcctgaa tcgccgcgtc ctgcttgctg agccaccgcc accgcagtgt
     5281 cggccgcgat tcgtatccgc gccggaccgc cagcgcatcc acgaccacga tctgcaccgc
     5341 cgccgcgacc gggatcgcca gcaatgcgcc ctccggcccg agcaactccg tgccggccag
     5401 gaccgcggcg aacacggtca gcggcgacaa accgaccgcc tctttcgtca cgcgcggcac
     5461 gatcaccgcg tccacaacca tctggcgagc caggatgaac ccgactacgg ccacggccag
     5521 caatggtgaa accgtcagag ccaggaacac cgccggcacg ccgccaatcc acggcccgat
     5581 gatcgggatc ggctccgtca gtccagccac caatcccagc agcggccaat acggcagccc
     5641 catcaggccg aacacgatca tcgagacaat cccaacgatt accccgagga tcagccggct
     5701 ccgcaaccac ccgccgatcg actcctcgac cgcgtcccac agccccgcta tgcgcaatcg
     5761 gctccgcggc ggcacattct caaggagcat catccggagc agatccttct cgagcatgta
     5821 gaagtaggcc atgaccaggc aggccaaccc ggcgatgatc cagagcgccg tcgtgcccag
     5881 ggcggagagc accgctttcg gcggcgcctc cgatggtggc gcttccagct cgacgatcgc
     5941 ccgctccacg atgtcgcgac ccggcccgct caggagcgga ttcccgcttg tcgcccactg
     6001 ctggtcgagt gtccgcaacc ggtcgggttc ctcggctcga aaccggtcga cctcccggct
     6061 cgcggccggc gcgattgcga ggatcggcaa aacgagcccg gccacgatcg cgaggtcgac
     6121 caggatgatc gacgccgccc gcggcacgcc atgtcgtccc aggcggcgga ccggccgctc
     6181 gatcaccgtc gcgtacagga acgccagcag cccgatctcg accacgagcc ggaccttgaa
     6241 caggacataa aacagcagca gggtcaggaa gagtccccac ggcgacaacg gccgccatcg
     6301 ctccggcgtc ggtccggatc gtgcaatgcc cagattggac ggccgctcgg tcatgtcgac
     6361 gtctcgcctg gcatcgagtt acagtcctgt accgcaccca tactacgcct cggccgatcc
     6421 ctcgacctca accgcgggca gttgccggta ttgcaggatc gaagtgccaa tgtaggccgc
     6481 catgccgagc ggtccgcaca gtaccgtcag ccagatcgcg ttcccgatcc cgatccactg
     6541 gccgacggct ccgccgacga gcgtcccgag cggatacgcg ccccggatca cgaaccgcac
     6601 gaccgaggtc aaccggccgc gcatctcccg cggcatcagg gccagccgga gcgagagctg
     6661 gttgatgttg ttggtcggct cgatcacgcc ttccaggaag agcatcccgc tgatcaccgc
     6721 cacggcatgc caatcgagca tcaccgccgc cgcgatcacg atcgccgtca gcgtcagccc
     6781 gacattcggc cagacgatcg cgttgccgac cccgacacgc cgcgccaccg gtccgggcag
     6841 gaacgcgccg atcgcgaacc cgatcgccgc gaccgtgaag accaggccat acgcgccggc
     6901 actcaacccc ttggtgtcga gcaggaagac ccgcagcaac ggctcccgga tcgagtagaa
     6961 gaagacgccg agaccgccgc tcaaaccgat cgcgcgcagg atcggatccg agaagaggta
     7021 ccggaacccc gcccggatct ctgagaggat tgatgtctga acagccgtgg ccggcggctc
     7081 ttcgaccgtg atctgtccga tcagcgccgc ggacccggcg aacgagacgg catcgatcag
     7141 gacagccgcc ggcgcgccga cgagctggac cagaccaccg cccgcgaccg gtccggccac
     7201 ctccgccatc gcttcgacct gtgtgatggc cgatgccccg gcaatcattt cgctgcccgg
     7261 cgtgatcatc ggcagcagcg attgccaggc gatcatgtgc caggtggtga agacgcccgc
     7321 caggaatccg attgcgtaca acccgggcat ggacagcatg tcgagccagg cggcaatggg
     7381 caccgccagc agcacgagcg cccggccggc atcgcaaatc atgatcaccg gccgcttcgg
     7441 gagccggtca atccacgcac ctgcgaccaa cgtaaagagt aactgtggca actgttcgaa
     7501 cgtcagcagc agtcccacat ggaacggtcc cgcgccgagc gtcgtcacgg cgatgatcgg
     7561 cagcgccagg cgcgtgacct ggtcgcccgc cgcggacacg gccgacgccg cccacagcct
     7621 gagaaaggcc ggagatcgcc agagctctcc ggccggtcgc cgcgctatcc gggtcaacat
     7681 gacgcgcatg atactggtcg aggtcgactg acttaaacct actaagcttt gcgtagaatt
     7741 ggaccagatg gataccgcgg cgggcgcgga cgtatacgcg tgccgtgtgt ccggtgaatc
     7801 agtattaaca gcgcgccatt ttccgtagtc gacagggtat ggtgaagaat tctgggcgcg
     7861 gccgagggtt cccggcgcgc gcaggtcagg tacacgtgag cacgagccaa agcgagcgtt
     7921 ccatgcgggg aaacatcacc gggcgctcag acagatgatg agtcgcattt cgacccgagt
     7981 ccgcttcatc cttgcgatga tgctgctcct tgtcccgatc gtcgccatcg cggcgatcat
     8041 cctcgacgag agcttcaagc gaagccagga acagatcatc atcagcgagt tcgcgactgc
     8101 ggacgtcgtc tcgcagagcg tcgccgagct gatccgtggg cagaaggaag ccctgtccgt
     8161 gctcgcagaa gaacagtcgg tgcggtcgct cgaccagggc aaggagcagg cgctcgccct
     8221 catggacgac taccgggtca gtcggcagcc ggttaatggc gtgtttctgc taaagaatga
     8281 cttgaccgtc gtcgcccaat ccggcggcat cgatatctca tccctgccgc cggaattcaa
     8341 cgacgccgcc aaggccgccg tcgacggcgg ccaggtaaca gtctccgact ccctgctgtt
     8401 gtccaacggc gaagtcaagg tgatcgcgat catcgtgccg gtctacgcga gtaccgaagc
     8461 gtccagcacg gagatgccat ccggcgcgat cggcgccttc tactccgtgg agcgactgat
     8521 cgccgggttc cagccgcccg ccggcttcac gaccggctcc aacgtcgcca ttgcgctggt
     8581 cagctcgacc ggcaggatca tcagcgttcc cgggcgcacc agcgacccga gccagatctt
     8641 ccaggacggc ccggccctga acaacgccat caagtcggcg ctcgccagcc agcgcacgcg
     8701 caccaccttc caggatccga ccggcctgca ccgcgtcacg gtcggcgttc cgatcgatat
     8761 gcccggcaat gactgggcag tcctcgtctc cggaccggaa accaccgcgt tcggtccgaa
     8821 caagatgctg atcgagcggg cccttctcgc cctgatcggc tcggttgtga tctgcttcct
     8881 cctcgccctg ctgttcagcg aatggctgac acggccgttc cgcctcctga ccagccaggc
     8941 caaggcgctc agcaccggag cggtcgtcct cgacctcgaa cctgtcggcc caagcgacgc
     9001 cgctatcctg agccgcacga tccgcgagat ggctgaccgc ctgcgtgccc agatccgcga
     9061 caccgaagcg gcacgcgagg agatcgctcg ccaggcagag cgcctgcgcg acctgctccg
     9121 ccgcaccgtt cggctccagg aagacgagcg ccgccgcatc gcgaccgaca tccacgacgc
     9181 cgtctccccg ctcatcaccg gcgccctcta ccaagcccag gccgtgcgca tcgcgcgctc
     9241 tggctcaggc aacgggaatg gcaacggcca tgatggcgta aacggtcatc accccgacca
     9301 ggaagaggcg aacgagggcc tgcaggaagt cggcgagctg ctcgagcggg ccatgcgcga
     9361 gctccatgac gtcatcttcg atctccgccc gcccgatctc gacgacatcg gcctcgaagc
     9421 cgcgattcag cgccacgtcg atcaggtcaa tcgcaccggt ctgcgctgct cgctcgaggt
     9481 cgtcggcgag gagcgccgcc tctcgcccga ggcccgcctc gccatctatc gcatcgtcca
     9541 ggaagccctg cacaacgcca tccgccacgc ccgcgccgac gaatccctcg tccgcatcga
     9601 gtggctggcc gatcgcctcc gcgtcaccgt ccaggacgac ggctccggct tcgagaccga
     9661 aggctccggc tggcgcgccg gcctcggtct gatgagcatg cgcgagcgtg ccggctcgat
     9721 cggcgccgcc ttcgaagtcg tctcccggcc gggcaccggc accgccatcg ttctcgagcg
     9781 aatgaacgac ggcctcgacc tcaccgccga gcatccggtc ctcgatccgg agctcgcccc
     9841 gtcgcagtcg cccgccgatg gcgatatagt tgacgaagac aacgttgacg tcggtgagga
     9901 acacttggtt cagtgatttc agttctgctc gtagatgatc atccggtcgt ggtggagggc
     9961 ctgcgcaagc tgttgacgca gactggagag gtcgtgatcg ccgccgaggc gaacgacgcc
    10021 gccaccggca tcaagctcgc caagcagatc cagccggatg tcgtgttgct cgacctgcgc
    10081 atgcccggcg cgaccggcat ccaggccgtc cgccgcatgc gcgagcagga agtccgctcc
    10141 gccgtcatca tcctgacctc gtatggtgat caggcctatg tccgccaggc gatcgaggcc
    10201 ggcgccgatg gctacctgct caagagcacg ccgccggacg atctgatcca gtcgatccgc
    10261 aacgccgccc gcggtcgcaa accgctcagc cctgagctga tcgacggcgt cctgaccgac
    10321 ttctccggtc tcgcccggga acaaacccgc cgcaacgccg acctgaccga cgatgaccgc
    10381 gagatcctca ggcgcgcctc cgaaggcgcg accaacaagg agatcggctt cgccatgggc
    10441 cgcagcgaga tcgccatcaa gaagcgcctc caggtcatct tctccaagct cggcgccgtc
    10501 gaccgcgccc acgccgtcgc cgaatccatt cgccgcggcc tcatctagcg atcgacagtt
    10561 gtttcatttc gtagtggcgg ggctccgtac ccgccttgag gtgccgaata acaggctcac
    10621 tgatgaccaa cccgttatca gcggtgcgat gcacacgaaa accgcgataa ggtgagtcca
    10681 ggcgggtacg gagccccgcc actacgtctc cggtcgcaca ctaacggaaa acggggacgg
    10741 tctggtggcc gtccccgttc gctatcgtca cacccgtttc cgggtgatta ctggctcaac
    10801 gacttcgacc gatccccgag cgtcaccgaa accgtcatat ccttgtcgcc gcgcttcacg
    10861 acaacatcga tcgtgtcgcc cggcttgtag ttgaacaacg cctcggagaa ggtcgtctgc
    10921 gcgttcagct catagtcccc gaccttcgtg atcacgtcgc cctgctgaat cccggcatcc
    10981 gccgccggac cgctcggcgg cacgtcctgg acgatcacgc cctggtccgt cgagaggccg
    11041 agctgcgccg ccgccgcggc cgagttggac acgtacgcga tgccgaagta cgggtaggtc
    11101 accttgccgc tcttgatcag ctcatcggtc accttcttga cgctgttcga cggaatcgcg
    11161 aagaacagcc cctggatcgg ttgaccctga ttatcgaccg agatgcccag cgtgttcacg
    11221 ccgatcacct cgccattgaa gtcgatcagt ggcccgcccg agttgcccgg gttgatcgcc
    11281 gcgtcgtgct ggatcaggtt gttgtactgg ctctgggtcg atccgtcgcc ggggaagtcg
    11341 cggttcgtcg cgctgacgat gccctcggtc accgtgttct ggaagttgcc gagcggcgag
    11401 ccgatcgcca gcaccgactc gcccacctgg accgcgtcgg aatcacccag cgcaatcgtg
    11461 ccgggcatgt cgccgtcgac ctggaccacc gccaggtcgc tgatctcgtc cgagccgatc
    11521 agcttggccc cctttgtcgt gccatcggaa aagacgacct ggaacttcga gccgccgttc
    11581 accacgtgcc agttcgtcac gatgtgcccg tcgttgtcga tgatgaaccc ggtgcccgag
    11641 cccaccggct gcagcgacga gccgccgttt tgcatgccga cgcgctgctc attgacgacg
    11701 gtcaccacgg cgggactgac ctgcttgacg acatcgaccg ccgacgacat ctgtccggac
    11761 ccggtcgagg attcagccgt cggcgacgcc tcctgcagcc gggcgaccgt cgacggcgta
    11821 tcctgggcca tcgctccccc gaaatcgatc gctcccgtcc ccgccagcac gagcatcagc
    11881 agcatgccga tggtcagact caccgtcatg atccaccgct cacgtcgatt catgtgattg
    11941 aaactccttt tgatgatctg cggcggtgga caattggatc cgtgcgttca tctttgcgat
    12001 tgacggaacg ccgccaccaa ccgatagtga tgcgcgatca tgaaccttgc atgaagaggt
    12061 gcaccgcccc atgagcactg ccgctaccga cctgctgcgc gacttcgagg cgttgttcgc
    12121 gcgaaccgaa gtggttgatt tatcggccgt tctcgaggaa ggcgcgccga tctggccaac
    12181 ccatccccca ttgataatcc atcgcaccgt gacccatgag cgccacggct acttcacgaa
    12241 ttcgatcttc atgcccgagc acgtcggcac ccattgcgac gccccggccc atgccattcc
    12301 cgagctcatg gaccagacca tcgaccgctt cccgatcgac aggatgatgg gtcccgcctg
    12361 cgtgatcgac gtcagcgccc gcaacctcga agctggcgag atgctgaccg cccaggacat
    12421 cctcgactgg gaagccgagc acggccaaat ccgcccgggc gacatcgttc tgatcaactt
    12481 cggctggttc gcccgccact ggcggaccga tgagcaggct cgctactacg tcacccatca
    12541 gcccggcctc gacgaagccg ccctcaagct catctacgag cgcggcgcca cagccctcgg
    12601 ctccgataac accaataccg aaactccgat ccgcgacggc aaatccctca cccccagcta
    12661 tgcccatacc acctacttcc tgcccaacgg catccccttg atcgagagcc tcgccaacct
    12721 cgaacgcctc ccgccccact gctacttcat cgccctccct ttgaagatca aaggcggctc
    12781 cggctcccca attcgcccaa tcgccctgat tccagaggga taggttttag gttctgggtc
    12841 tcaggttttg ggggatgcgg ggttcgggag gcggaccggg ccgcccacgt ctggcgtaac
    12901 gccggccccc cgtggccggc tggggtcgcc gaacggccgg ttgatggtgg tcgatacccc
    12961 ctcatagacc atctcgccgg taccgaagcg gcccaggggc gggccgcgct acgtcttggt
    13021 gccattggaa cggactaggc atccttagcc tgcttcggca ggcttattgg atgtagccct
    13081 gggatttatc cccgggcgcc gccgggaaat accatcgcat gcaccgcacc ggccatcgcg
    13141 aaatcagggt cgcattgcca tgccatgcga cggcataagt tcgcctcccg actgctcgcc
    13201 tgctcgcctc ccgactgctc gcctcatgta ccatcattcc aacggcttat cgcttcatca
    13261 cgaaaggatt cgcaccgcat ggttgagaag accgcatcag cgaaa
//
LOCUS       DATAOZ010000400         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6999784, whole genome shotgun sequence.
ACCESSION   DATAOZ010000400 DATAOZ010000000
VERSION     DATAOZ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6999784"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VHR64_10185"
     CDS             complement(<1..243)
                     /locus_tag="VHR64_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303238.1"
                     /translation="MRFGTFAPQGWRGDLREIADPIEQYEAMTNVAKVADMGLWDSVW
                     LYDHFHTVPTPELMTTFECWTATATLARDTERVKVGQ"
     gene            complement(260..1774)
                     /locus_tag="VHR64_10190"
     CDS             complement(260..1774)
                     /locus_tag="VHR64_10190"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEX3303239.1"
                     /translation="MADKQQANLARLKELIGEVHDLGHAGAVLSWDQLVMMPEGGGVA
                     RGHAMETLARIMHEKFTSDEVGNLLDKLEGYAASLPEDDDDRCLIERSKYHRDRERRI
                     PTELAGELAGFEATHVPIWLEARKQQDYTIFAPSLQKGVDLHKRLIDCFDATESPYDV
                     LLEQHEIGMTTAEVKALFDNVRAGLKPLIEKVKANPDAVDDSPLNEQYDVATQAALVL
                     ELSRGIGTTEDAWRLDPTVHPFETRFANTDIRLTTKYVENDLAVSVGGVNHEFGHGLY
                     ERQIAPELERTGLNHGASLGLHESQSRTWENQVGRSEAYWEWAYPIYAKHFPEQAKKY
                     DATAIYRAMNKMFPSLIRTESDQLTYGMHIILRFEIEQEMINGTLAIKDVRDSWNERM
                     NDLIGVEVPNDTQGVLQDIHWAGGGIGYFSAYLIGNVISAQAWEMVERDIPNIADQFR
                     KGEFGALRDWQKEHLHRFGSKFSPRATTEKFLGGQLTHEPYVRYLTGRVNEFYG"
     gene            1947..2258
                     /locus_tag="VHR64_10195"
     CDS             1947..2258
                     /locus_tag="VHR64_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303240.1"
                     /translation="MFRLATVPQFAGRRFGKLAVVNRPAPRTAVTEQAARPTTADIRL
                     RRFAESTVNEDTYTAILPSEGNSPIDLLRVITEAMKDLPEFQIQGEEDERFVLTLTLK
                     A"
     gene            complement(2363..3439)
                     /locus_tag="VHR64_10200"
     CDS             complement(2363..3439)
                     /locus_tag="VHR64_10200"
                     /inference="COORDINATES: protein motif:HMM:NF020318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HEX3303241.1"
                     /translation="MPTTVANPLGTTDSAETILAIDIGAGTQDILVYDPEQTPENSFR
                     LVMPSQTQIIGQRIRRVTDAGRPLHLVGQVMGGGASTAAIKTHLDAGLPVTATSSAAR
                     TIHNDPQRVRHLGVWITDEAPRGAVELELGDLDLDAIETALNAFGVELPERYAIAVQD
                     HGVRIGQGNNDVRGDYLHWLVNSAGDLRRAAFLTPPEEMTRMQAIHLLAPGAIVMDTG
                     AAAVLGALRDPMVAQAAEEGAVLVNLGNMHTFATLVRRNRVLGVFEHHTGGLDRDLLG
                     RLVERLRYGELTNREFRERFDGHGAVVSEDYRSFSPFRFIAVTGPNRSIAAGLNWHQA
                     APYGDMMLTGAFGLVDGYLAAAGA"
     gene            complement(3420..>3961)
                     /locus_tag="VHR64_10205"
     CDS             complement(3420..>3961)
                     /locus_tag="VHR64_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303242.1"
                     /translation="AQPSESPMQSGEPGARPSRLVPRRFQVNRNGQADQPATDSVEQP
                     VPEYMPVERTAAPRQQPESPLIDPDEELPAWNAPSRAIPVPEPYIPPAPRVASRSESR
                     ELPRPETGSGFESSLFADLDVPAGDEAEVAGPFQSRLLNELTSSDDEPIEGAADVLLD
                     EFSLPSRNQPGARDADNGR"
BASE COUNT          645 a         1357 c         1248 g          711 t
ORIGIN      
        1 ctggccgacc ttgacccgct cggtatcgcg ggccagggtc gcggttgcgg tccagcactc
       61 gaaggtcgtc atcagctccg gcgtcgggac ggtgtggaaa tggtcgtaga gccaaaccga
      121 gtcccagagc cccatgtcgg caactttggc gacattggtc atcgcctcgt attgctcaat
      181 cggatcggcg atctcgcgca gatccccgcg ccacccttgc ggcgcgaacg ttccgaatcg
      241 cattgccagc cctttcagcc tatccgtaga actcgttgac ccggccggtc agataccgca
      301 cgtatggctc gtgcgtgagc tggccgccga ggaacttctc ggtggtggcg cgcgggctga
      361 acttgctgcc gaagcggtgc aagtgctcct tctgccagtc gcgcagcgcg ccaaactcgc
      421 ccttccggaa ctggtcggcg atattcggaa tgtcgcgctc gaccatctcc catgcctgcg
      481 cgctgatcac gttgccgatc aggtaggccg agaagtagcc gatgccgccg cccgcccagt
      541 ggatgtcctg gagcacgccc tgcgtatcgt tcggcacctc gaccccgatc agatcgttca
      601 tccgctcgtt ccacgagtcg cgcacatcct tgatggccag cgtgccgttg atcatctcct
      661 gctcgatctc gaagcggagg atgatgtgca tgccgtaggt gagctggtcc gattcggtcc
      721 ggatcagcga cgggaacatc ttgttcatcg cccggtagat cgcggtggcg tcgtacttct
      781 tcgcctgctc ggggaagtgc ttcgcgtaga tcgggtaggc ccactcccag taggcctcgc
      841 tgcggccgac ctggttctcc caggtccggc tttgcgactc gtgcagaccc agcgaggcgc
      901 cgtgattgag accggtccgc tcgagctccg gcgcaatctg ccgctcgtag aggccgtggc
      961 cgaactcatg attgacgccg ccgaccgaga ccgccagatc gttctcgacg tacttggtgg
     1021 tcaggcggat gtcggtattg gcgaagcgag tctcgaacgg gtggacggtt gggtccaggc
     1081 gccaggcatc ctcggtcgtg ccgatcccgc gcgagagctc gagaacgagg gcggcctggg
     1141 tggcgacgtc gtactgctca ttcagcggcg agtcgtcgac cgcgtccggg ttggccttga
     1201 ccttctcgat cagcggcttg agaccggctc ggacgttgtc gaagagcgcc ttgacctcgg
     1261 ccgtcgtcat cccgatctcg tgctgctcga gcaggacatc gtagggagac tcggtcgcat
     1321 cgaagcagtc gatcagccgc ttgtgcaggt cgacgccctt ctgaagtgac ggcgcgaaga
     1381 tcgtgtagtc ctgctgcttg cgcgcctcca gccagatcgg cacatgcgtc gcctcgaacc
     1441 cggccaactc gccggccagt tcggtcggaa tgcgccgctc gcggtccctg tggtacttcg
     1501 accgctcgat caggcagcgg tcatcgtcat cctcgggcag cgacgcagcg tacccctcga
     1561 gcttgtcgag caggttgccg acttcgtccg aggtgaactt ctcgtgcatg atgcgagcga
     1621 gggtctccat ggcatgtccg cgggcgactc cgccgccctc cggcatcatc acgagctggt
     1681 cccagctcag gaccgcgccc gcatggccga gatcgtggac ctcgccgatc agctccttga
     1741 gccgcgccag atttgcctgc tgcttgtctg ccaagggatt tcctccatcg aagcattacg
     1801 gaagcgcggt atccgcgcca gttgcgtccg caagcctacc agcagaaatt gaaattggcg
     1861 gtgttgacct cttgacacag gaacacttgt tcgatagatt gcgtacgtac aggtgttcga
     1921 aacgagtgta ctagcaggag acaaagatgt tccgactcgc aaccgttccc cagttcgccg
     1981 gccgccgctt cggcaagctc gccgtcgtca atcggcccgc tccccgcacc gccgtgaccg
     2041 agcaggcagc acgcccgacg actgccgaca tccggcttcg ccgattcgcc gaatcgacgg
     2101 tcaacgagga cacgtacaca gcgatcctgc cgagcgaggg caactcgccg atcgatctgc
     2161 tccgcgtcat taccgaggcc atgaaggatc tgcccgagtt tcagatccag ggcgaggagg
     2221 acgagcgctt cgtcctgacc ctcacgctca aggcctgatt ccccgacccg aacgccccgc
     2281 cggagcgcgc ccaaccccgc tccgcccgca cccgcgcccc gcattccgcc gggaagggcc
     2341 ccgcaccctc cccgccccgc gctcaggcgc cggccgccgc caggtaacca tcgaccagcc
     2401 caaaggcgcc ggtcagcatc atgtccccat acggcgccgc ctggtgccag ttcaatcccg
     2461 ccgcaatgct ccggttcggt ccggtgaccg cgatgaaccg gaacggcgag aacgagcggt
     2521 agtcttcgga aaccaccgcg ccgtgaccat cgaaccgctc gcgaaattcg cggttcgtca
     2581 gctcgccgta gcgcagccgc tcgaccaatc gaccgagcag atcccggtcg agcccaccgg
     2641 tgtgatgctc gaagacgcca agcacgcgat tccgccggac cagcgtcgcg aaggtgtgca
     2701 tgttgccgag attgaccagg accgcgccct cttcggccgc ctgcgcgacc atcgggtccc
     2761 gcagcgcccc cagcacggct gccgcgccgg tgtccatcac gatcgcgccc ggcgcgagca
     2821 ggtgaatcgc ctgcatccgg gtcatctcct ccggcggcgt caggaaagcg gcccggcgca
     2881 ggtcaccggc cgagttgacc agccagtgca ggtagtcgcc ccggacgtcg ttgttgccct
     2941 gcccgatccg gacgccatga tcctggaccg cgatcgcgta ccgctccggc aactcgacgc
     3001 cgaacgcgtt cagggccgtc tcgatcgcat cgagatcgag gtcgccgagc tcaagctcga
     3061 ccgcgccccg cggagcctcg tcggtaatcc agacacccag gtgacggacc cgctgcggat
     3121 cgttgtggat cgtgcgggcg gctgacgatg tcgcggtgac gggcaacccg gcatcgaggt
     3181 gcgtcttgat tgccgccgtc gatgctcctc cacccatcac ttgtcctacc aaatgaagcg
     3241 gccggccggc atcggtcacc cggcggatcc gctggccgat gatctgggtc tgggacggca
     3301 tgaccagccg gaagctgttc tccggcgtct gctcgggatc gtagacgagg atgtcctgcg
     3361 tcccggcgcc gatatcgatc gcgaggatcg tctccgcgct gtcggtcgtg ccgagcgggt
     3421 tagcgaccgt tgtcggcatc acgcgcgcct ggctggttgc gggacggaag cgagaactcg
     3481 tcgagcagca catcagccgc accctcgatc ggttcgtcgt cggaacttgt caactcattg
     3541 agcaggcgcg attggaacgg tccggcgacc tcggcctcat cgccggccgg cacatcgaga
     3601 tcggcgaaca ggctggactc gaatccggaa cctgtctccg gccggggcaa ctccctcgac
     3661 tccgatcggg acgccacccg gggcgccggc gggatgtacg gttccggcac cgggatcgcg
     3721 cgagatggcg cgttccacgc cggtaactcc tcatcgggat cgatcagcgg ggattcaggc
     3781 tgctggcgtg gcgctgctgt ccgctcgaca ggcatgtact ccggaacagg ctgctcgaca
     3841 gagtccgtcg cgggttgatc cgcctggccg ttccggttca cctggaagcg gcgcggcacc
     3901 agccgcgatg gtcgcgcgcc cggctctcct gactgcatcg gagattcgct cggctgtgcg
     3961 c
//
LOCUS       DATAOZ010000401         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_951156, whole genome shotgun sequence.
ACCESSION   DATAOZ010000401 DATAOZ010000000
VERSION     DATAOZ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951156"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VHR64_10210"
     CDS             complement(<1..641)
                     /locus_tag="VHR64_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX3303243.1"
                     /translation="MRIYFDHNATTPVDPAVAETIARVLTGEFGNASSVHHFGQRAKA
                     ILDEARSSVAALIGAEPSEIVFTSGGTESDNFALRGVAEALEPTGRKHLIASSIEHEA
                     VLVTLRALARRGWRTTLLPVDHTGIVNPKDLLEAITDDTAIVSVMHANNEIGTIQPVA
                     ELARIAHERGAVFHTDAVQSVGKIPVDIRALGVDLLSLSAHKFNGPKGTGALW"
     gene            930..1322
                     /locus_tag="VHR64_10215"
     CDS             930..1322
                     /locus_tag="VHR64_10215"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX3303244.1"
                     /translation="MNIGKSVVIKGELNGSEDLTIEGQVEGKIELRQNVLTIGANARI
                     KAQVFAKSVIILGEVTGNVSASEKVDIRDNGSVDGDIAAPRVAIAEGAHFRGSIDMQK
                     GSGQKSEPKQAAAAATSAGQPAAAGANR"
     gene            1410..2069
                     /locus_tag="VHR64_10220"
     CDS             1410..2069
                     /locus_tag="VHR64_10220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3303245.1"
                     /translation="MLSAILRWGGKRSGQPDPSGESSAAKGGDALVPSKALPKFLAAI
                     SHQSESPVLVDLGPVIGANVSFFGEQLGCKLFIEDLFADYERHAKAGTLDDMATGMDK
                     RFRHEDGTVDGILCWDIFDYLPKAASQALASQIVRMLKPGGSVMGFFCSSAPPAKTPF
                     TKYEIADEKSLRHRQHPGSGGAKHVLLNRDIIRMFDGLVVSDSFLLKSNTREILLKRR
                     S"
     gene            2077..>2812
                     /locus_tag="VHR64_10225"
     CDS             2077..>2812
                     /locus_tag="VHR64_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX3303246.1"
                     /translation="MRPVIALLSDFGTRDHYAGTMKGVMIGICPDVTLIDITHDITPH
                     DVMEGALQLAASCRYFPLGTIFLVVVDPGVGSLRKGIAAEAGEYRFVAPDNGVLTAVF
                     KELPPKRVVELTERRYARPTVSRTFEGRDRFAPAAAWLAKGIQLSALGRSITSYQQVE
                     IPQPVAGDALAGVVLLIDRFGNLVTNIDRRTFEAFSRGQPVALSVAGHPVAGVVATYA
                     DIHSGEVCALFGSSEHLEFAVNGGSAA"
BASE COUNT          530 a          887 c          875 g          520 t
ORIGIN      
        1 atccatagcg ccccggtgcc cttcggtcca ttgaacttgt gggccgagag cgacaacaga
       61 tcgaccccca gcgcacggat gtccaccgga atcttgccaa ccgactggac cgcatcggta
      121 tgaaaaacgg cgccacgctc gtgcgcaatg cgggcgagct cggctaccgg ctgaatggtt
      181 ccgatctcgt tgttcgcatg catcaccgac acaatcgccg tatcgtcggt tatcgcctcc
      241 agcaaatcct tcgggttcac gatgcccgtg tggtccacgg gcaggagggt ggttcgccag
      301 ccgcgacgcg ccaaggcacg cagcgtcacg agcaccgcct catgctcgat gctgctggcg
      361 atcaggtgct ttcgcccggt cggttcgagc gcttcggcca cgcccctcag cgcgaagttg
      421 tccgactcgg ttccgccgct cgtaaacacg atctccgatg gctcggcgcc gatcagggca
      481 gccaccgatg agcgcgcctc atcgaggatc gccttggcgc gctggccgaa gtggtggacg
      541 ctcgaggcgt ttccgaattc gccggtcagg acccgcgcga tggtttcggc gaccgccgga
      601 tcgacagggg ttgtcgcgtt gtgatcgaag tagatacgca tgtcccgatc gatccatggt
      661 agcattcgct ctaactctca gcaataaaag gactagacag cgggcaaagc ccgtgcgtat
      721 actgtcgcga ccctgccaaa gccgcggagc tgaaagacaa catcgatagg tggagagtgc
      781 caatgtggaa acgggatgat gcggcaaagc caagcgcacc ggccggccag gggcctgccc
      841 cggcgcagca cagtaatgcg ccgctaaccg cggagtcgac gtcgcgaccc acgaccgtcg
      901 aaccccagcg tggacaggag agaaatgtcg tgaacatagg caaatcggtc gtcatcaaag
      961 gcgagctcaa cggcagcgaa gatctcacga ttgagggaca ggtcgaaggc aagatcgaac
     1021 tgcgtcagaa cgtgctcacg atcggtgcca atgcacgcat caaggcacag gtgtttgcca
     1081 agtccgtgat catccttggc gaggtaaccg gcaacgtcag tgcgtccgaa aaagtcgaca
     1141 tccgcgacaa cgggtcggtg gacggcgata ttgcggcgcc acgggttgcc attgccgaag
     1201 gggcgcattt ccgcggcagt atcgacatgc agaaggggtc gggacagaaa tccgaaccca
     1261 agcaggccgc cgcggccgcc acgtcggcag gccagccagc ggccgccggg gccaaccgct
     1321 agccatcgca tcggcctccg ggcggtcgcg tgatctcgcc actcgtctgt ggcggggccg
     1381 gtcgtccggt cgcccgatcg tcgatctcca tgctgtcagc cattctccgc tggggcggca
     1441 aacgttccgg ccagcccgat ccgtccggtg agtcgtcggc cgcgaaaggc ggcgatgccc
     1501 tcgttccgtc gaaggcactg ccgaagtttc tcgccgcgat ctctcatcag tcggagtccc
     1561 ccgtcctggt tgacctcgga ccggtcatcg gcgccaacgt gtcctttttt ggcgagcagt
     1621 tgggctgcaa actgttcatc gaggatctct tcgccgatta cgagcgccac gcaaaggccg
     1681 gtacgctcga cgatatggcg accgggatgg ataagcggtt tcggcacgag gacggcacag
     1741 tcgacggcat cctgtgctgg gacatcttcg attacctgcc gaaagctgcg tcgcaagcgc
     1801 tggcgagcca gatcgtccgg atgctgaaac ctggcggctc ggtcatgggc ttcttctgca
     1861 gttctgcacc gccggcgaag acccctttca cgaagtacga aatcgccgac gagaagtctc
     1921 tccgccatcg tcagcatccc ggcagcggcg gcgcgaagca cgtcttgctg aatcgcgaca
     1981 tcatccgcat gttcgacggg ctggtcgtgt cggattcgtt cctgctgaaa agcaataccc
     2041 gcgagatttt gctgaagcgc aggagctagc cagaccatgc gtccggtcat cgctcttctg
     2101 agcgacttcg gcacgcgcga tcactacgca ggcacgatga agggtgtgat gatcgggatc
     2161 tgtcccgacg tcacgctgat cgatatcacc cacgacatca ccccgcacga cgtgatggag
     2221 ggggcacttc agctggctgc ttcctgccgc tactttccct tgggaacgat ctttctcgtg
     2281 gttgtcgatc ccggtgtcgg ttcgcttcgc aaaggcattg ccgccgaagc cggcgagtac
     2341 cggttcgtgg cgccggacaa cggtgtcctc accgccgtat ttaaagaact gcctcccaaa
     2401 cgggtggtcg agctcactga gcgccgttat gcccgtccga ccgtgagccg cacgttcgaa
     2461 ggccgcgatc gatttgctcc ggcagcggcc tggctggcca aaggcatcca gctctcggcg
     2521 ttgggtcgct cgattacgag ctatcagcag gtcgagattc cacagcccgt ggctggggac
     2581 gcgctcgccg gagtcgttct gctgatcgat cgattcggca acctggtcac aaatatcgac
     2641 cgccgcacgt tcgaagcctt ctcgcgtggc cagccagtcg cgctcagtgt cgccggccat
     2701 ccggtcgcgg gcgttgtcgc cacctacgca gatatccact ccggcgaggt gtgcgcgctc
     2761 ttcggtagca gcgagcatct ggagtttgcg gtcaatggcg gcagtgcagc cg
//
LOCUS       DATAOZ010000402         7735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7001425, whole genome shotgun sequence.
ACCESSION   DATAOZ010000402 DATAOZ010000000
VERSION     DATAOZ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7001425"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VHR64_10230"
     CDS             <1..305
                     /locus_tag="VHR64_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX3303247.1"
                     /translation="LRHADAIIRHEIEESGEDVWQFFGVLLPVNSVGVMGDFRTYGRV
                     CAVRAVTSEDAMTADWARLPYDVLGRISNRIVNEVRGITRVVYDITSKPPATIEWE"
     gene            complement(333..941)
                     /locus_tag="VHR64_10235"
     CDS             complement(333..941)
                     /locus_tag="VHR64_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303248.1"
                     /translation="MARTRLAVVFLLALTWIGLAGTVGAQSRVVSRGKVTGPTALVRF
                     ETTDGCMLFEGIVFAEQDVRSGLTAGVTAAIVDTCTGTDTALNYGTTTVADFTVRPNL
                     AGATLHALIPVTDLKSGATATMQVDVNWMASGPIDRMHNAYRYEEGGLTITAHSVQWT
                     RPASATGMITDGTTVFASSGSSDAFSLLLWTNYGEVLLSLPG"
     gene            complement(1081..1785)
                     /locus_tag="VHR64_10240"
     CDS             complement(1081..1785)
                     /locus_tag="VHR64_10240"
                     /inference="COORDINATES: protein motif:HMM:NF003989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX3303249.1"
                     /translation="MQTIRPPRARSARDSQGQIPSAGSAYENEIPDVVIRRLPIYVRT
                     LQILIDQTGSESVSSDQLAEAIGVSGAQIRRDLSYFGRFGKQGKGYDARRLVEEINRI
                     LRLDQTWEVALVGFGNLGRAIAQYGGFVPSTFRITCIFDRNEERVGQTVDGIVVESDA
                     RIAKSIRELGVKIGIIAVPQTSAQVVADALVEGGIEAILNYAPCHLRTPESVTVRDVD
                     PVGAMQSMTYYLSNGE"
     gene            1906..2772
                     /locus_tag="VHR64_10245"
     CDS             1906..2772
                     /locus_tag="VHR64_10245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX3303250.1"
                     /translation="MRIGVTFPHFDIGPDRDAIRSYVQTTVDCGYDYLLAYDHVLGAD
                     TINRPGWTGYTKDNIFHEIFTLFGYVAALAPDLELVSSVVILPQRQTALVAKQAAQID
                     ILTAGKFRLGVGVGWNAVEYEALGEDFRTRGRREEEQIELLRCLWTEPVITFRGEFDT
                     VTEAGLNPLPIQRPIPIWIGGTTEVPIKRAARLGDGWFPMGPIDDAQHAKLQLLRETA
                     ESVGRDPKEIGIDARIDTFRTGEENWEQNIAAWKEAGATHLCVNAMNLGLDIDSHRAL
                     LERFATVAMPYR"
     gene            2999..4624
                     /locus_tag="VHR64_10250"
     CDS             2999..4624
                     /locus_tag="VHR64_10250"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3303251.1"
                     /translation="MVEPATQPAPTTAEQAMPLLRMSGISKSFPGVHALQDVSLEIYP
                     GECLALMGENGAGKSTLMKVLSGVYSPDAGTVEIQGTPVTLTNPHQAQQLGISIIYQE
                     FNLFPNMSVDENVFIGRDPSRGGMVDRRALRRDTRELLDRLGVRLDPGALVGDLSVAQ
                     QQMVEIAKALSYNARIVIMDEPTSALTDTEVTALFKIIRGLKQQNLGVIFISHRIEEV
                     VEICDRITVLRDGHNAGEIVTADATPARIVQMMVGRPLSDLFQKEVSEAVGDPVLEVR
                     GLSRAPAKGDASSTWIQNISFTVRSGEIVGLAGLVGSGRTELARSIFGADRIDSGQIL
                     VEGKPVSLKHPLDAIELGIGLVPEDRKQQALVLGLPVRENISLANLDAVTRAGFVRRS
                     AERSIAQRFIDALQIRTPGMEQKVVNLSGGNQQKVVLAKWLALNPKVLIVDEPTRGID
                     VGAKAEVHLLLSRLAQQGVGILMISSELPEILGMSDRILVMREGRIAGELSRAEATQE
                     IIMSMATGLEHVRAAATDLIDLAEISAEQKGNG"
     gene            4629..4997
                     /locus_tag="VHR64_10255"
     CDS             4629..4997
                     /locus_tag="VHR64_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303252.1"
                     /translation="MAGSITGNVIPAPGAMVSPQAPGMIPNRTTVPTHVDEPIPPATV
                     KVRPLTQYGTAEELLGVFKSKPMAQLEPSDFEQAPASPEEMRKALPAELTPIRPLAPL
                     EHNIAVDAGPTTPELESGPS"
     gene            5028..6047
                     /locus_tag="VHR64_10260"
     CDS             5028..6047
                     /locus_tag="VHR64_10260"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303253.1"
                     /translation="MGASSAAPATIGEEQRGIDYAGLVGKFAPVIFLLLVVTFFSFFE
                     SGFRTTNNFLNIIRAVSFFGILAVGMTFVILTAGIDLSVGSALAFCGIVCASAAKGSR
                     ALLSGKDEGGVHVLYAFLAAIGVGLLIGLIHGVFIAYLNVPAFIVTLGGLGAWRGATL
                     LWSNGQPISSFSKDFTHWGKQDIGQVPIPVIVFLGMGLIAHIVLKYTRYGRWVYALGG
                     NAEAARLSGLNTRQITSSVYVISGFCAGVSGFLLTSRLNSAEQVAGQSYELQAIASVV
                     IGGTSLFGGSGGVLGTLVGAVLIGVLNNGLVQMNVSAYYQPIVIGVIIVLSVYVDQVV
                     KSRRR"
     gene            6145..7173
                     /locus_tag="VHR64_10265"
     CDS             6145..7173
                     /locus_tag="VHR64_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3303254.1"
                     /translation="MSKSISEVTQRRATRRTVIKTAAVAGLAVPFMPRMAGAQAKYKL
                     AFSVPGLNFPFFVHMMDLAKKHVEEKYPDVEFIALDGQENGAPSSTKQSNDIDAIIAQ
                     KVNALVISPNDVAALAPAVQAAVDAGLVVVTVDRNVTDVNTLAHVGADNVEGGRLQGR
                     YLIEILPDGGDIIELQGQPGAAPAIDRHKGLDEILSAQDKIKVVVSQTAEFARAKAVS
                     VLEAALASNPDPKAVVCANDDMAFGAIEALSDAGKTNVPIIGFDALPEALQDIQAGTQ
                     AATVEQFPGGQSTQAIDIALAKLIDGKDPAQHDTYLTPILITKDNLGEAERAVEAGVA
                     TPVASPTS"
     gene            complement(7235..>7735)
                     /locus_tag="VHR64_10270"
     CDS             complement(7235..>7735)
                     /locus_tag="VHR64_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3303255.1"
                     /translation="QVIRTLLTTGQVDHEGRFYTFRDCELHPRGPRPDGLPILIGALQ
                     SGPRMLRLAARYADQWNGWLVHGRSHPDELPPLLGALDAACESVDRDMGTLVKTLGIM
                     VDQRPEGQRASTGTAQPLTGSDEEIAAAIRQFVAHGISHIQLVPTIQGIEGINRLADL
                     FPLLED"
BASE COUNT         1430 a         2479 c         2436 g         1390 t
ORIGIN      
        1 tgctgaggca tgccgacgcg atcatccgac acgagatcga agagagcggc gaggacgtct
       61 ggcagttctt cggcgtcctc ctgccggtca actcggtcgg cgtgatgggt gatttccgca
      121 cctacggccg ggtctgtgcg gtccgggccg tgaccagcga ggacgcgatg acggccgatt
      181 gggcccgcct gccgtacgac gtcctcggcc ggatcagcaa ccggatcgtc aatgaggtcc
      241 gcggcatcac ccgcgtcgtc tacgacatca cctcaaagcc ccccgccacc atcgagtggg
      301 agtagcgcgc gacgtcggta cggtggccct tactacccgg gcaaggaaag caacacctcg
      361 ccgtaattcg tccatagcaa gagggaaaac gcgtcgctcg agccagatga cgcaaacaca
      421 gtcgtaccgt cggtgatcat gccggttgct gaggcgggac gcgtccactg aacagaatgg
      481 gccgtgatgg tcagtccgcc ctcctcatac cggtatgcgt tgtgcatacg gtcgatcggt
      541 ccgctggcca tccaattcac gtcgacctgc atcgtcgccg tcgccccact cttcaaatct
      601 gtgacaggga tgagggcatg tagcgttgca cctgcgaggt tgggacgcac cgtgaagtcg
      661 gcgacggtcg tcgttccgta attgagggcg gtgtcggtac cggtgcaggt atcaacgatg
      721 gcagctgtta ctccggccgt tagcccggag cgaacatctt gttcagcaaa gacaatgcct
      781 tcgaagagca tacaaccgtc tgtggtctca aagcggacca gcgccgtcgg accggtaact
      841 tttcctctgg agactactcg agattgagct cccaccgtcc cagccagacc gatccaggtg
      901 agagcaagaa ggaaaaccac ggcaaggcgg gtgcgggcca tcttctatct cccttcacga
      961 gccacggcaa ttctgctttt cacgggtgat gtgatcaggc cacgctattg aaagggcatg
     1021 cgcgagtcaa ggggtcaccg aaaaatacgg gaaaatcccg tgttttcggc aaaaccgatt
     1081 ctattcgccg ttggagaggt agtaggtcat cgattgcatt gcaccgacgg ggtccacgtc
     1141 gcggaccgtg accgactccg gcgtgcgcag gtggcagggc gcatagttga ggatcgcctc
     1201 gatgccgcct tcgaccaggg catcggccac gacctgggct gaggtctggg gtacggcgat
     1261 aatgccgatc ttgactccga gctcccggat tgatttcgca atcctggcat cgctctcgac
     1321 gacgatcccg tcgacggtct ggccgacccg ctcctcattg cgatcgaaga tgcaggtgat
     1381 ccggaaggtg gacggcacga aaccgccgta ctgggcgatc gctcgaccga ggttgccgaa
     1441 gccgaccagc gcgacctccc acgtctggtc cagccgcagg atgcggttga tctcctctac
     1501 caggcggcgg gcgtcgtacc ccttgccctg cttgccgaac cgtccgaaat aggagaggtc
     1561 gcgccggatt tgcgcgccgg acacgccgat cgcctcggcc agctggtcgg atgagaccga
     1621 ctcggagccg gtctgatcga tcaggatttg cagcgtccgc acgtagatcg gcaagcgccg
     1681 gatgacgaca tcggggattt cgttttcgta ggcgctaccg gccgaaggga tttgtccctg
     1741 gctgtctcgg gcactgcggg cgcggggtgg tcgtatcgtt tgcatcgtgc ggccgttccc
     1801 ggtagtggcg gtcgaatcgc gcgaacgcac agcgagctcg ttcgtgcgag atttcacaag
     1861 tatggtttgc cgaaggcagt agcgtcaaaa caagaggagc agattgtgcg tatcggcgtt
     1921 acattcccgc atttcgatat cggtcccgac cgcgatgcca tccggagcta cgtccagacg
     1981 accgtcgact gcggctacga ctacttgctt gcctacgacc atgtcctcgg cgcggacacg
     2041 atcaatcggc ccggctggac cggatacacc aaggacaaca tcttccacga gatctttacc
     2101 ctcttcgggt acgtggccgc cctcgcgccc gatctcgaat tggtttcgag cgtcgtgatc
     2161 ctgccccaga ggcagaccgc gctcgtcgcc aagcaagcgg ctcagatcga tatcctgacc
     2221 gccggcaagt tccggctcgg ggtcggcgtc ggctggaacg cggtcgagta tgaggcgctc
     2281 ggcgaggact tccgcacccg cggccggcgc gaggaggagc agatcgagct gctccgctgc
     2341 ctctggaccg agccggtcat caccttccgc ggcgaattcg acaccgtcac cgaggccggg
     2401 ctcaatccac tgccgatcca gcggccgatc ccgatctgga tcggcggcac gaccgaggtc
     2461 ccgatcaagc gcgccgcccg gctcggcgat ggctggtttc ccatgggtcc gatcgacgac
     2521 gcccaacacg cgaaattgca actgctccgc gagacggccg aatcggtcgg tcgcgatccg
     2581 aaggagatcg gcatcgacgc gcggatcgat accttccgca ccggcgagga gaactgggag
     2641 cagaacatcg ccgcctggaa agaggccggc gccacgcacc tctgcgtcaa cgcgatgaat
     2701 ctcggcctgg acatcgacag tcaccgcgcc ctgctcgaac gcttcgcaac ggtcgcgatg
     2761 ccatatcggt aatcggtgcc agacgtacat attcgtagcg cgtggccccc gtgctccgct
     2821 atggccgcgg gccatgatgg tccggccgaa acggcggttg gcatgaatcg acaagcgccc
     2881 ggagagacca gcggagcacg gcggccccgc gctccgtttt atcgcccgat tgacagacga
     2941 cttctactcc ggtagaaacc ggaacattcg gccgggcaaa tcggcagcga gagaaccaat
     3001 ggttgaacca gcaacccaac ctgcgccgac gaccgccgaa caggctatgc cgctcttgcg
     3061 catgtcagga atcagcaaga gcttcccagg tgtccacgcg ctccaggatg tctcactcga
     3121 gatctacccc ggggagtgcc tggccctgat gggcgagaac ggcgccggca aatcgaccct
     3181 gatgaaggtc ctctccggcg tctattcgcc ggacgccggc acggtcgaga ttcagggcac
     3241 gccggtcacg ctgaccaatc cgcaccaggc gcaacagctc ggcatctcga tcatctacca
     3301 ggagttcaac ctcttcccga acatgtcggt cgatgaaaat gtcttcatcg gccgggaccc
     3361 aagtcgcggc ggcatggtcg atcgtcgcgc gcttcgccgc gatacccgcg agctgctcga
     3421 tcggctcggc gtccggctcg atccgggcgc gctggtgggt gatctgagcg ttgcccagca
     3481 gcagatggtc gagatcgcca aggcactctc ctacaacgcc cggatcgtga tcatggatga
     3541 gccaacctcg gccctgaccg acaccgaggt gaccgcgctc ttcaagatca tccgcggcct
     3601 caagcagcag aatctcggcg tgatcttcat ctcgcaccgg attgaggagg tcgtcgagat
     3661 ttgcgatcgg atcacggtcc tccgggacgg ccacaatgcc ggcgagatcg tgacagccga
     3721 cgccacaccg gccaggatcg tgcagatgat ggtcggccgg ccgctctcgg atctcttcca
     3781 gaaagaggtc agcgaggcag tcggagatcc ggtgctcgag gtccggggcc tttcccgggc
     3841 cccggccaag ggcgacgcct cgtccacctg gattcagaac atcagtttca cggttcgttc
     3901 cggcgagatt gtcgggctgg cgggcctggt cggttccggc cggactgagc tggcgcgctc
     3961 gatcttcggc gccgaccgga tcgacagcgg ccagatcctg gtcgagggca agccggtttc
     4021 gctcaagcac cccctcgatg cgatcgagct tggcatcggc ctcgtgccgg aggaccgcaa
     4081 gcagcaggcc ctcgttctcg gccttcccgt gcgcgagaac atcagcctgg ccaaccttga
     4141 cgcggttacc agggcaggct ttgtccggcg gtcggccgaa cggtcaatcg cccagcgatt
     4201 catcgacgcg ctccagatcc ggacgccggg tatggagcaa aaggtcgtca acctcagcgg
     4261 cggcaatcag cagaaggttg tgctggccaa gtggctggcc ctcaacccga aagtgctgat
     4321 cgtcgacgag ccgactcgcg gcatcgacgt cggcgccaag gcagaagtgc acttgctcct
     4381 tagccggctc gcacagcagg gcgtcggaat ccttatgatt tcctccgagc tgccggagat
     4441 cctcggtatg agcgaccgca tcctggtcat gcgcgagggc cgaatcgccg gcgagctcag
     4501 ccgagccgag gcaacgcagg aaatcatcat gagcatggcg accgggctcg aacacgtccg
     4561 ggccgcggcc accgatctga tcgaccttgc ggagatctcc gccgaacaga agggaaatgg
     4621 gtaacacgat ggcagggtca attaccggta atgtgattcc ggctccaggc gcgatggttt
     4681 cgccgcaggc accgggcatg attccgaacc gaaccacggt tccgacgcac gtcgatgagc
     4741 cgattccgcc ggcgaccgtc aaggttcggc cgctgacgca atacggcacg gctgaggagc
     4801 tgctcggggt cttcaaaagc aagccaatgg cccagctgga gccgtccgac ttcgagcagg
     4861 cgccggcctc gccggaagag atgcggaagg cgctgccagc tgagctgaca ccgatccggc
     4921 cgctggctcc gctcgagcac aacattgccg tcgacgccgg cccgacgaca cctgagctgg
     4981 aatccggccc gtcctagcat ccgctcatca ggggaaggtt cagcagcatg ggcgcatcga
     5041 gtgcggctcc ggcgacgatc ggcgaggaac agcgcggcat cgattacgcc ggtctggtcg
     5101 gcaagtttgc gccggtaatc ttcctgctcc tggtggtcac ctttttctcc tttttcgaat
     5161 ccggcttccg cacgaccaac aatttcctga acatcatccg cgcggtctca ttcttcggga
     5221 tcctggcagt cggcatgacg ttcgtgatcc tgacggccgg catcgacctg tccgtcggct
     5281 ctgcgctggc cttctgcggc atcgtctgcg catcggccgc caagggttcc cgggcgctct
     5341 tgtcgggcaa ggatgagggt ggagtccacg tactctacgc cttcctcgcc gcgattgggg
     5401 tcgggcttct tatcgggttg atccacggag tcttcatcgc atatctcaac gtgccggcct
     5461 tcatcgtcac actcggtggt ctcggtgcat ggcgaggcgc aacgctgctc tggtccaacg
     5521 gtcagccgat cagcagcttc tcgaaggact ttacgcactg gggcaagcag gatatcggcc
     5581 aggtgccgat cccggttatc gtcttcctcg gaatgggcct gatcgctcat atcgtgctca
     5641 agtacacgcg gtatggacga tgggtctatg ccctgggcgg caacgcggag gcggcgcggt
     5701 tgtccggctt gaacaccagg caaattacct cgagcgttta cgtgatcagc ggcttctgcg
     5761 ctggggtatc cggattcctc cttacttccc gcctcaactc ggccgagcag gtcgccggcc
     5821 agtcatacga gctccaggca atcgcgtcgg tcgtgatcgg cggcaccagc ctgttcggcg
     5881 gctccggcgg cgtactcggg acattggtcg gcgcggtcct gatcggcgtg ctcaacaatg
     5941 gcctggttca gatgaacgtc tcggcgtact accagccgat cgtgatcggc gtgatcatcg
     6001 tgctcagcgt gtatgtggat caggttgtaa aatcgcggcg tcgctaacgc ccgggaacct
     6061 gccggttttc aacaccattg gccatccttg gctcgctccg caccctgcgc gggcgattcg
     6121 tgctcattga ggaggattag ggccgtgtcg aagtcgatct cggaagtcac gcagcgccgg
     6181 gccactcgcc ggacggtcat caagaccgcc gcggtcgccg ggctcgccgt tccgttcatg
     6241 ccacgcatgg ccggagctca ggccaagtac aagctcgcct tctcggtccc gggactcaac
     6301 ttccccttct ttgtccacat gatggatctg gccaagaagc atgttgagga gaaatacccg
     6361 gacgtcgagt tcatcgcact cgatggccag gaaaacggtg cgccgagctc caccaagcag
     6421 tcgaacgaca tcgacgcgat catcgcccag aaggtgaacg cgctggtgat cagcccgaac
     6481 gacgtcgctg cgctggcccc ggctgtgcag gcagccgtcg atgccggtct ggtcgtcgtg
     6541 acggtcgacc gcaacgtcac cgacgtcaac acgctggccc acgtcggtgc ggacaacgtg
     6601 gagggagggc gactccaggg ccgctatctg atcgaaatcc tgccggacgg tggcgacatc
     6661 atcgagctgc agggtcagcc gggtgcggcg ccggcaatcg accgccacaa gggtcttgac
     6721 gagattctct cggctcagga caagatcaag gtcgtcgtca gccagaccgc ggagttcgcg
     6781 cgggcgaagg ccgtatcagt gctcgaggct gcgctggcct cgaatcccga tccgaaggca
     6841 gtcgtctgcg ctaacgacga catggcattc ggagcgatcg aagcgctgtc cgacgctggt
     6901 aagaccaacg tgccgatcat cggcttcgac gccctgccgg aggccctgca ggacatccag
     6961 gccggtaccc aggccgcaac ggtcgagcag ttccccggcg gtcagtcgac ccaggcgatt
     7021 gacattgcgc tggccaagct gatcgacggc aaggacccgg cccagcacga cacctacctg
     7081 acgccgatcc tgatcaccaa ggacaacctc ggtgaggcag agcgcgcggt cgaggcaggc
     7141 gtcgccacgc cggtcgcgag cccgacctcg taagcacccg ccgacgtgaa caacggaccc
     7201 cggtgatgtc accggggtcc gttctgtttg tccgtcaatc ctcgagcaac gggaagaggt
     7261 cggcgaggcg gttgatgccc tcgatgccct ggatcgtggg cacgagctgg atatggctga
     7321 taccgtgcgc cacgaactgg cggatcgccg ccgcgatctc ctcgtcggac ccggtcaatg
     7381 gctgcgccgt cccggtgctc gcccgctgcc cctccggccg ttggtcgacc atgatcccca
     7441 acgtcttgac cagcgtgccc atgtcacggt ctactgattc gcaggccgca tcgagcgcgc
     7501 ccaggagcgg cggcaactcg tccgggtggc tgcgaccatg gaccagccag ccgttccact
     7561 gatcggcgta gcgcgccgcc agtcgcagca tccgaggacc tgattgcagc gcgccgatca
     7621 ggatcggtag cccgtccggt cgcggaccgc gcggatgcag ctcgcaatcg cggaaggtgt
     7681 agaaccggcc ctcgtggtct acctgcccgg tggtcagcag agtgcgaatg acctg
//
LOCUS       DATAOZ010000403         5798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2531669, whole genome shotgun sequence.
ACCESSION   DATAOZ010000403 DATAOZ010000000
VERSION     DATAOZ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531669"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1006
                     /locus_tag="VHR64_10275"
     CDS             233..1006
                     /locus_tag="VHR64_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303256.1"
                     /translation="MHTNMTRRQVIVGLGAAALMGEVRLDKVGAAATPEAGTPYAPEI
                     DPAKFTTTIDNPFMPAMPGMRWVYDGESDGAKQVNTIFVTAETKVLMGVTCVAVHDTV
                     EEEGALTEDTIDWFAQDTDGNVWYFGEDTKELENGKVTSTEGSWQSGKDGAYPGIIMP
                     AKPTPDGPYRQEYLAGEAEDMAKVIKLDGKISNSYGDFTDVLVTEEWTPLEPGVLERK
                     YYVSGIGMVSSEIIKGGDEHFDLTTFEQGSATPEATPGS"
     gene            complement(1083..1565)
                     /locus_tag="VHR64_10280"
     CDS             complement(1083..1565)
                     /locus_tag="VHR64_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303257.1"
                     /translation="MHALRVSLALSLCVLFTLTPGRSTAAAGTCVINGTTYTATIEGC
                     GVITGTDGDDVNRGSNVADQISGLGGNDIICGRGGADSIDGGDGDDTMSGGDGNDYLV
                     GSFGDDQIDGNAANDRLYGQYGNDLLIGGDGDTDRCSGGVGSDSTDDPTTCEVIFGIP
                     "
     gene            1842..2006
                     /locus_tag="VHR64_10285"
     CDS             1842..2006
                     /locus_tag="VHR64_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303258.1"
                     /translation="MGEQEPSTFEAVTRQMVETLAEELREIRHRVDGLLWMGAGAILL
                     DTVLRLAGVR"
     gene            2195..3679
                     /locus_tag="VHR64_10290"
     CDS             2195..3679
                     /locus_tag="VHR64_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303259.1"
                     /translation="MSAKIDAFSPDIVALSHESFPRYALRSYQIEPARAITESIKEKL
                     GKQFVVVFSRQSGKDELLAQVLVWTLLTHAETGGTAVIAAPTLRPQGAISRDRLIARM
                     RELLPNDVVTREGHIVELGKASARFLSALPTANVRGQTADLLLVANEAQHIQPSVWDP
                     VFDPMAASTNATTLFMGTPWSRDSLLARQTQHLREIERETGERRVWKVPWTVVAEVIP
                     KYRERVEARIAQFGERHPTIRTEYWLEELDGEEGLFPPHRLAQMQGDHPRRYEAEPGK
                     RYALLLDVAGEDEGGGSAEAFASVARRDSTALTVIEIDVAHGRMPIYRAVNRRRWIGT
                     KHAHLRDALADLARNVWQAERVVIDATGIGAGLAAFLAERLEDRSQGRVIPVDRFVFS
                     EKSKSQLGWDFVGLIETGRYKEYVDPAPVGSVERVDTEAFWAELKRIEFAVAAGPGQA
                     MRWGASGRGHDDLVMSAALVARLEGVRWQRRIAVGSREGAEARS"
     gene            3739..4065
                     /locus_tag="VHR64_10295"
     CDS             3739..4065
                     /locus_tag="VHR64_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303260.1"
                     /translation="METEELVVGGVDFDDVEQLRAESIAIVRDLILKGNPDLVPDLVR
                     GETLAELMDSVEPARAAYQRIAESVRTLVPVVPAGGAGTSVAIEELSADGLIKRGMEQ
                     LRRGVA"
     gene            4352..5239
                     /locus_tag="VHR64_10300"
     CDS             4352..5239
                     /locus_tag="VHR64_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX3303261.1"
                     /translation="MGLTKVEAAKLTQDLMVRGVIDTIVRDSPLFQVLPFMDVVGSAV
                     TYNREETNPAASFYDVGDTWSEATPTFTQVTAPLRIMGGDADIDRFLQQTYADPNDLE
                     VEIIKKRAKAVAHLFGDAFYNGDSATNPKSFDGLTKVLSGTSQEMSLGPNGGALALDA
                     LDQMIDLIPGGRPDALLMSKRSRRKLSSLRRSSGNLLETTVDAFGRHVVMYDGIPVLA
                     DDNVSDTQTQGSATQCSSIYAVRFGMEGVMGLQNSELQVVDVGDLETKDSGRWRIKWY
                     ASAAVFSMLGVARVKGILP"
     gene            5361..5675
                     /locus_tag="VHR64_10305"
     CDS             5361..5675
                     /locus_tag="VHR64_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303262.1"
                     /translation="MLNYERYAPSPDGALADRSITITTGGTAQDAAPANPNRVYLLIQ
                     NPSGNSGSVWFAIGTTATAASPAIELQPGQAWESPSSFCPTGAVSVIHSTTGTKVTVK
                     EA"
BASE COUNT         1115 a         1666 c         2044 g          973 t
ORIGIN      
        1 tgtttgacag acgccaagcg cgcctgatgg gttccggcaa cgatacgaat cacctgtcct
       61 ggggatccgc ggctagccta catccgggcg cgcggccgga caagacactt cgttggccca
      121 ttggtagagc gtgcttcatc cagccttcat cagatttcaa tctgccttcg tagagtgagg
      181 ggatcggtgg atttcgttca ccggaaacgg cgatggaaag gactggatcc cgatgcatac
      241 gaacatgaca cggaggcagg tcattgttgg tcttggcgcg gccgcattga tgggagaagt
      301 tcgcctggac aaggttggcg cggcggccac gcccgaagcc ggaaccccgt atgcgccgga
      361 gatcgacccg gcgaagttca cgacgacgat cgacaacccg ttcatgccgg ccatgccggg
      421 gatgcgctgg gtttatgacg gagaatccga tggcgcgaag caggtcaaca ccatctttgt
      481 cactgctgag acgaaggtgc tgatgggcgt gacgtgcgtc gcggtccatg acacggtcga
      541 ggaggaggga gcgctgaccg aggacacgat cgactggttt gcccaggata cggacggcaa
      601 tgtctggtac ttcggcgagg acaccaagga gctcgagaac gggaaggtga ccagcaccga
      661 aggatcgtgg cagtcgggca aggacggcgc ctatcccggc atcatcatgc cggccaaacc
      721 gacgccggac ggtccctacc ggcaggagta tctcgcgggc gaggccgagg atatggccaa
      781 ggtgatcaag ctcgacggca agatctcgaa ctcgtacggc gatttcacgg atgtgctggt
      841 gaccgaggaa tggaccccgc tcgagccggg tgtgctcgag cggaagtact acgtgagcgg
      901 gatcgggatg gtctcgtcgg aaatcatcaa ggggggcgac gagcatttcg atctgaccac
      961 gttcgagcag ggctcggcca ccccggaggc gacgcctggg tcctgagccg ggatcccgcg
     1021 acacgacaac ggccgggacg aacttcgccc cggccgttgg tttgttcgat gttacggact
     1081 ggttacggga ttccgaagat gacctcgcag gtggtcgggt cgtcggtcga gtcgctgccg
     1141 acgccgcccg agcagcggtc ggtatcgccg tcgccgccga tcaggagatc gttgccgtac
     1201 tgaccgtaga ggcggtcgtt ggcggcgttg ccatcgatct gatcgtcacc gaatgagccg
     1261 accaagtagt cgttgccgtc accgccggac atcgtgtcgt cgccatcgcc gccgtcgatc
     1321 gagtcggcgc cgccgcgacc gcagatgatg tcgttaccgc cgaggccgga aatctggtcg
     1381 gccacgttcg agccgcgatt gacgtcatcg ccgtcggtgc cggtgatgac gccgcagccc
     1441 tcgatcgtgg cggtgtaggt cgtgccgttg atgacgcagg tgccggccgc ggcagtcgac
     1501 cggcccgggg tgagcgtgaa aagcacgcag agggacaacg cgagactgac acgcaaggca
     1561 tgcatacgag tcctccccaa ctagattcgg atgtttgttt ggtgtggaaa acattgttac
     1621 cgtatctcag aatgtcaacg gggggaaaca tccaaaccgg accaggacgt tagtactccc
     1681 agctcaacca acgagcagga acaccggctc ggcgggcctc atgatcggga cggtaaaaaa
     1741 tcggaaattc aaggctaaat cgtcacgaaa tcgagcctcg tgcgctatta gttatagaac
     1801 acctgttctt cgcagacgga gagttgagag aggagggggc aatgggggaa caggagccat
     1861 cgaccttcga ggcggtgacc cgccagatgg tcgagacgct ggccgaggaa ttgcgcgaga
     1921 tccggcaccg ggttgacgga ctgctctgga tgggcgcggg cgcgatcttg ctcgacacgg
     1981 tcctgcggtt ggcgggggtg aggtgaggcg agcaggcgag gaggcgatta ggcgagcagt
     2041 cgaggagtcg ggttgatggt ggttggtggt tggttgatgg aacggaacag gagcgggtag
     2101 ttcgtcccga aaaccgaatc ccgaaaaccc aaaacctgga acctaaaacc taaaacctac
     2161 gaggtgcggt gtggcgaggg cgagtgcgga tgcattgtcc gcgaagatcg atgccttcag
     2221 tcccgacatc gtcgcgctct cgcatgaatc gtttccccgc tacgccctcc gctcgtatca
     2281 gatcgagccg gcccgggcga ttaccgagag catcaaggag aagctcggca agcaattcgt
     2341 cgtggtgttc agccggcaat cgggcaagga cgagttactg gcccaggtgc tggtctggac
     2401 gctgctgacc catgccgaga cgggcgggac ggcggtgatc gccgcgccga cgttgcggcc
     2461 gcaaggagcg atcagccggg atcggttgat cgcgcggatg cgggagctgc tgccgaacga
     2521 cgtggtgacc cgcgaggggc acatcgtcga gttgggcaag gccagcgcgc gattcctctc
     2581 ggcgctgccg actgcgaatg tgcgcgggca gacggcggac ctgctgctgg tcgcgaacga
     2641 ggcacagcac atccagccgt cggtctggga cccggtcttc gatccgatgg cggccagcac
     2701 caacgcgacc acgctcttca tgggcacgcc ctggagccgg gactcgctgc tggcgcgaca
     2761 gacgcagcac ctgcgcgaga tcgagcgcga gaccggcgag cggagggtct ggaaggtccc
     2821 gtggaccgta gtggccgagg tgatcccgaa gtaccgggag agggtcgaag cgcggatcgc
     2881 ccagttcggc gagcgccacc cgacgatccg gaccgagtac tggctcgagg agctggacgg
     2941 cgaagagggg ctctttccgc ctcaccggtt ggcccagatg cagggagatc atccccggcg
     3001 gtatgaggca gagccgggca agcgatatgc gttgctgctc gacgtggccg gagaggacga
     3061 gggcggcgga tcggccgagg cattcgcgtc ggttgcccga cgagatagca cggccctgac
     3121 cgtgattgag atcgacgtcg cgcatgggcg aatgccgatc taccgggcgg tcaaccggcg
     3181 gcgctggata gggacgaagc acgcgcactt gcgggatgcg ttggccgatc tggcccggaa
     3241 tgtctggcag gcggagcggg tcgtgatcga tgcgaccggg atcggcgccg ggttggccgc
     3301 gttccttgca gagcgactgg aggatcggtc gcagggtcgg gtgatcccgg tcgaccgatt
     3361 cgtcttctcc gagaagagca agagccagct cgggtgggac ttcgtcgggc tgatcgagac
     3421 gggccggtac aaggagtacg tcgatccggc gccggtgggt tcggtcgagc gagtggatac
     3481 cgaggcgttc tgggcggagc tgaagcggat cgagttcgcg gtggcggcgg gtccgggaca
     3541 ggcgatgcgc tggggtgcat ccgggcgggg acatgacgac ctggtgatga gcgcggcgtt
     3601 ggtggcgcga ctggagggcg tgcggtggca gcggcgcatc gcagtcggga gtcgcgaagg
     3661 cgcggaggcg cgaagttgag gcgagggatg gattgagaaa acccaaaacc tggaacctaa
     3721 tacccaaagg gaggactaat ggagaccgag gagctggttg ttggcggggt cgattttgac
     3781 gatgtcgagc aattgcgggc ggagtcgatc gcgatcgtgc gggatctgat tctgaagggg
     3841 aatccggatc tggtgccgga tctggtgcgc ggggagacgc tggccgagct gatggattcg
     3901 gtcgagccgg cccgggcggc gtatcagcgg atcgcggagt cggtgcggac gttggttccg
     3961 gtcgtgccgg ccggtggcgc ggggacgtcg gtggcgatcg aggagttgtc ggcggacggg
     4021 ttgatcaagc gggggatgga gcaactgagg cgcggagtcg cgtaggcgcg gagttgtcgc
     4081 gtggcatggc aatgcgacct gggtttcggg agaacgcgtg cgatgcatgc cgtggggtgt
     4141 ccgggcgccg cccggcgata aatcaccggg ctacatccaa ttagcctgct gaagcaggtt
     4201 gcccgctctc cctctcgcga aatctggaga aggggatgct tggcgtcgcg ctggcagcac
     4261 accgggcggc gccctccgtt aactcccaat cactaaccac caaccactat ccactgttat
     4321 tggaatttca tatgcaggag aacgcataca catgggattg acgaaggttg aggcggcgaa
     4381 gttgacgcag gatctgatgg tgcgcggggt gatcgatacg atcgtccgcg attcgccgct
     4441 atttcaggtg ctgccattca tggatgtggt cggcagcgcg gtgacctaca accgcgagga
     4501 gaccaacccg gcggcctcgt tctacgacgt cggtgatacg tggagcgagg cgacgccgac
     4561 cttcacgcag gtcacggcgc cgctgcggat catgggtggg gatgcggata tcgaccggtt
     4621 cctgcagcag acctacgccg atccgaacga tctcgaggtc gagatcatca agaagcgggc
     4681 caaggcggtc gcccatctct ttggcgatgc cttctacaac ggtgactcgg cgacgaaccc
     4741 gaagtcgttc gacgggctga ccaaagtgct gtccggaacc agccaggaaa tgtcgctcgg
     4801 tccgaacggc ggcgccctgg cgctcgacgc gcttgaccag atgatcgacc tgatcccggg
     4861 cggacgaccg gatgcgctgc tgatgagcaa gcgctcgcgg cggaagctga gctcgctgcg
     4921 gcggtcgagc gggaacctgc tcgagacgac cgtcgatgcc ttcggccggc atgtcgtgat
     4981 gtatgacggc atcccggtgc tggcggatga caacgtgagc gacacgcaga cacagggctc
     5041 ggccacacag tgcagctcga tttacgccgt ccgcttcggg atggagggcg tgatgggatt
     5101 gcagaacagc gagctacagg tggtcgatgt cggcgacctg gagacgaagg attccggtcg
     5161 ctggcggatc aagtggtacg cgagcgcggc cgtcttctcg atgctcggcg tcgcgcgggt
     5221 gaaagggatc cttccttagg tcgtgagtcg tgaaacgtga gtcctgagcc gagtcaacgg
     5281 catcaaaccc tgacccaacc ccttcaggcg tgagccggcc gagcccaacc ggccggctct
     5341 tttcataggc aggagcccca atgctgaact acgaacgcta cgcgccctca cccgacggag
     5401 cactggccga tcgctcgatc accatcacca ccggcggcac cgcccaggac gccgctcccg
     5461 ccaacccgaa ccgcgtctat ctcctcatcc agaaccccag cggcaattcc ggctcagtct
     5521 ggttcgcgat cggcaccacc gcgaccgccg catcacccgc gatcgagctc caacccggtc
     5581 aagcctggga gtccccgagc agcttctgtc caaccggcgc cgtctcggtc atccacagca
     5641 cgaccggaac caaagtcacc gtcaaggagg cctgaccgat gcccttctcc caaggactgc
     5701 cagacggcgc cgtccgcgat cgcatcgccg gcacccgcaa cccgatcatc ccgctcggca
     5761 agccggcggt gatgagctcg ccgccgaccg tctcgacc
//
LOCUS       DATAOZ010000404         7100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5689154, whole genome shotgun sequence.
ACCESSION   DATAOZ010000404 DATAOZ010000000
VERSION     DATAOZ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5689154"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4745
                     /locus_tag="VHR64_10310"
     CDS             <1..4745
                     /locus_tag="VHR64_10310"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HEX3303263.1"
                     /translation="QSSVPQGYSPSDPVDQPVTVTAGETAQVQYVVSVAQASLLVSVV
                     DESGTAVAGACITVNGGTPVCDNDGVADGNGDTGQILISGLAPGDATVTMSTTPDGYT
                     DGGSTTVTLVAGQQSTVTFTLSVALGRIGLYTTNSNGEALGGACYSLNGGEPICDNGP
                     QDGDSDDGVMRLTGLTPGDYTISQTTVPDGYQQAPDQTITVNPGARTRVDFVNELALG
                     TLSITVTDDNGNAVPGACVTATDTAGNATQVCDNQDPDQNATDGVIAIPNLAPGDYTV
                     IVTSVPEGYQVPDVGQQSNVPAGGQGSAALPIAIIPPTETPTEVPSETPTEVPTETPT
                     EVPTATETPTEVPTATETATEEPTVTETATAEPTVTETVTEAPTETETATEAPTEEAT
                     STETATVEAATETPVATGSVRINVTDPDGNSLVGACFTVASDAASAEVCDNDPSDQEP
                     GEGIVEIGSLPVGDYTVHESKAPDGYTAPVDQTATITNDGDVVEVTFVNAETPVATGT
                     LRITSVDGENNPVGGGCYQISTTEVCDNQNNDAENAPGVIVIEGIPVGDYTVTETDAP
                     EGFQPAANQTVTIESNATAELTFTHEAAPKETGTVAMQLKDQDGNPAEGVCVLLSSEQ
                     NNISGQQYCDNGEGDQNPDPAMLEVTDLPVGSYSAQPVAQGETEPQAAAAAAASFDQS
                     TFTVKANVTIQVVVIIIIQLPEVGDLQITKRAADTNALQTGACFRVTGEGNEIEVCDN
                     DDVDLAGGNGVIRLEDLSAGSYTIEEITAPPGYQLAPVKTTTVPNGGTRSVLIKDQPV
                     ADTTGGLIVHKVDSDGNLLPGACFQLRDQGTPIATVCDSDDGANDGKTTFKDITVGDY
                     RLRETQAPSSQYLAGADTIVNIYGGVDDQVVEVVNTLKPGQIRITKVAEDGTTKLDGA
                     CFGLDRGSGIEYEACDNGSGDTNSTAGIILMINVPPDTWNLIETVPPAGYDPAADQSI
                     TVNPGQTLSLTVQNTPTPPSTDKGTVVINKMVGSDLAPGACFALKQGAVTKYSACDGS
                     NNDGVIEFPSVTVGNYTVHETKAPNPSSWYIIGADQNISVTKNNTTEIDIVNTLKPGR
                     ILVVKKNTSGNLLQNACFTITPDPNSAGQKCTNATGQVSFDNLPTTQTYKLTETKAPN
                     GYEKAKAVTNISLTPGLTTTVNVIDKKAPPPPTDGSVLVIKFFCPAGKAGEMTQYLDS
                     SDPGGAKLAQTSGCTKGDAKFSLVPKAGGTGIEFNTGADGQYFTTLAKGTWELREVSP
                     QKSDPEDVLIYAGQQTTVVVIDYVKPPEPKPATINILKYTCAPGFGGVYYADFLNNCT
                     ADSQLTNNVSFRTSGPTVAKRITGATGQPGRTSFTQLKAGDYTLFEDAPTTAVSVYAF
                     CGYDVYNWADWYSATGSIFLPLKEGANVTCIWFNVPDDLSDSTGAIVVHKYVCNGQNF
                     PSGFDYFANCPVETDNVKFAISFKQGNTFVPKTTGITNSNGILSFGRLKPGTYSLKEV
                     GGDWCNAKSDNVDAQGNLIVRAGQRTNVWIFNCVPTKQPPNTGSGAMAGVIGGGAVSS
                     AGATLIGFAWPILGVVGFRTRKKLRRAA"
     gene            4925..5350
                     /locus_tag="VHR64_10315"
     CDS             4925..5350
                     /locus_tag="VHR64_10315"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX3303264.1"
                     /translation="MDYSGWSGVGAFAPPVQKRFGGFWVRVVAYFLDAILLVIAQVIL
                     MQIIKNEATLQGVNFLLTWFYFAGLESMAGATLGKMVLGLSVTDEQGNNISFLRATGR
                     YFAKIISALILCIGYIMVAWDGRKQGLHDKMAGTLVVKN"
     gene            complement(5449..5598)
                     /locus_tag="VHR64_10320"
     CDS             complement(5449..5598)
                     /locus_tag="VHR64_10320"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX3303265.1"
                     /translation="MTKKVQCDCGFVARADDDDTLVEVVQKHAKDVHDMEMTREQVLA
                     MATPE"
     gene            5682..6443
                     /locus_tag="VHR64_10325"
     CDS             5682..6443
                     /locus_tag="VHR64_10325"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX3303266.1"
                     /translation="MGPLIRIYLAGQVGIECDGRLLSEWRAVGRQGRLAFAMLTAEHW
                     RAVPRQELIDEIWPDDPPPSSDRAVSAVMSKLRALMEREGRPEIEIAAAFGCYQMRLP
                     ANVWIDIEAAAEGIDQAEGFLRAGDFRAAWAPAQITCHIARRPFLQGDDGTWATRTRA
                     NLRDMLVRAYEALSGIYVRSGEPSNAVRYAKLATEAEPYRETTWQHLMHAHAVAGNRA
                     EALRSYECCRRLLSEELGISPSPQTEAVYLELLRS"
     gene            6592..>7100
                     /locus_tag="VHR64_10330"
     CDS             6592..>7100
                     /locus_tag="VHR64_10330"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3303267.1"
                     /translation="MQRANLSDLETTRFDLIVIGAGINGAAIARDAAMRGLSVCLLEM
                     NDLASGTSAWSSRLVHGGLRYLEHYELGLVRESLRERERLLRNAPHLVSPLPMVVPIQ
                     EGAKRGPLLIRAGMVLYDILSYDKSLPLHRMLSPEKALDRVPALDPEGLKAAAVYYDA
                     QVTFAERLVV"
BASE COUNT         1372 a         2269 c         2297 g         1162 t
ORIGIN      
        1 cgcaatccag cgtcccgcag ggctactcgc cgtctgaccc ggtcgatcag ccggtaaccg
       61 tcacggcggg tgagaccgcg caggtccagt atgtcgtctc ggtcgcgcag gcctcgttgc
      121 tggttagcgt ggtcgatgag agcggcacgg cagtggccgg cgcctgtatc accgtcaacg
      181 gcggcacacc ggtctgcgac aacgacggcg tcgcggacgg caatggtgac accggccaga
      241 tcctgatcag cggcctggcc cccggcgatg cgacggtcac gatgagcacc acgcccgacg
      301 gctacaccga cggcggctcg acgactgtca ccctggtggc tggtcagcag tcgaccgtga
      361 ccttcacgct ctcggtcgca ctggggcgga tcggactcta tacgaccaat tcgaacggcg
      421 aggcactggg cggtgcctgc tattcgctga atggcggcga gccgatttgt gacaacggcc
      481 cgcaggacgg tgatagcgac gacggcgtca tgcggctgac cggcctgacg ccgggcgatt
      541 acacaatcag ccagacaacg gtgcccgacg ggtaccagca ggcgccggat cagaccatca
      601 cagtcaatcc gggcgcgcgg acccgcgtcg acttcgtcaa tgagctggcg cttggcacgc
      661 tgagcatcac cgtcaccgat gacaacggga acgcagtacc cggcgcgtgc gtaaccgcga
      721 ccgatacggc cggcaatgcg acccaggttt gcgataacca ggatcccgac cagaatgcga
      781 ccgatggcgt gatcgccatc ccgaacctgg caccgggcga ctacaccgtg atcgtgacgt
      841 cggtgcctga gggctaccag gtcccggacg ttggccaaca gtcgaacgtt ccggcgggtg
      901 gtcagggctc ggccgcgctg ccgatcgcga tcatcccgcc gacggagacg ccgaccgagg
      961 ttccgtcgga aaccccaact gaagttccga cagaaacacc gactgaggtc ccgacggcga
     1021 cagaaacgcc gacggaagtg cctacggcga cggaaaccgc gactgaggag ccgaccgtca
     1081 ctgagacggc cacggcagag ccaacggtga cagagaccgt taccgaggcg ccgactgaga
     1141 cggagactgc aaccgaggct ccgaccgagg aagccacctc gactgagacg gcgactgtcg
     1201 aagcggccac ggagacgccg gttgcgaccg gcagcgtccg gatcaacgtc accgatccgg
     1261 acggtaattc gctggtcggc gcctgcttca cggtggcgtc ggatgctgcc tccgccgagg
     1321 tctgcgacaa cgatccgagc gatcaggagc caggcgaggg catcgtcgag atcgggtcgc
     1381 tcccggttgg cgactacacg gtccacgagt ccaaggcgcc ggatggctac accgcgccgg
     1441 tcgatcagac tgcgacgatc acgaacgacg gcgacgtcgt cgaggtgacc ttcgtcaatg
     1501 ccgagacccc ggtcgcgacc ggtaccctgc gcatcacgag cgtcgatggg gagaacaacc
     1561 cggtcggcgg tgggtgctac cagatctcga caaccgaggt ctgcgataac cagaacaacg
     1621 acgccgagaa cgcgccgggc gtgatcgtga tcgaagggat cccggtcggc gactacacgg
     1681 tcaccgagac ggatgcgccg gagggcttcc agccggcggc gaaccagacc gtcacgatcg
     1741 aatcaaacgc cacggccgag ctgaccttca cgcacgaggc cgctccgaag gagactggca
     1801 ccgtcgcgat gcagctcaag gaccaggatg gcaacccggc cgagggcgtc tgcgtgctgc
     1861 tgagcagcga gcagaacaac atctccggcc agcaatactg cgacaacggc gagggtgatc
     1921 agaacccgga tccggccatg ctggaagtga ccgacctgcc ggttggcagc tacagcgcgc
     1981 agccggtcgc gcagggcgaa accgagccgc aggcggccgc ggcagcagcg gcgtcgttcg
     2041 accagagcac cttcacggtc aaggcgaatg tgacaatcca ggtcgtcgtg atcatcatca
     2101 tccagctgcc tgaagtcggc gatctccaga tcacgaagcg agcggcagat acgaatgcgc
     2161 tccagaccgg cgcctgcttc cgggtaaccg gcgagggcaa cgagatcgag gtctgcgaca
     2221 acgacgacgt cgatctcgcc ggtggcaacg gcgtgatccg gctcgaggat ctctcggcgg
     2281 gcagctacac gatcgaggag atcacggcgc ctccgggtta ccaactcgcg ccggtcaaga
     2341 ccacaaccgt tccgaacggc ggcacccgca gcgtcctgat caaggaccag ccagtggccg
     2401 acacgacggg cgggctaatc gtccacaagg tcgattcgga tggcaatctg ctgccgggcg
     2461 cctgcttcca gctgcgcgac cagggcacgc cgatcgcgac cgtctgcgat tccgacgatg
     2521 gcgcgaacga cggcaagacg accttcaagg acatcaccgt cggcgattac cggctgcgtg
     2581 agacccaggc gccgtcgagc caatacctgg ccggcgccga caccatcgtc aacatctatg
     2641 gtggtgtcga cgaccaggtg gtcgaggtcg tcaacacgct caagcctggc cagatccgga
     2701 tcaccaaggt ggccgaggac ggcacgacca agctggacgg agcctgcttt gggctggatc
     2761 gcggatccgg catcgagtac gaggcctgcg acaacggctc gggcgatacc aattcgacgg
     2821 ccggcatcat cctgatgatc aacgtgccgc cggatacctg gaacctgatc gagaccgtgc
     2881 cgccggcggg ctatgatccg gccgcggacc agtcgatcac ggtcaatccg ggtcagacgc
     2941 tgagcctgac ggtccagaac acgccgactc cgccgagcac cgacaagggc acggtggtga
     3001 tcaacaagat ggtcggcagc gacctggctc cgggcgcgtg cttcgcgctg aagcagggcg
     3061 cggtcaccaa gtactcggcc tgcgacggct cgaacaacga cggcgtgatc gagttcccga
     3121 gcgtgacggt cggtaactac acggtccacg agaccaaggc gccgaatccg tcgtcgtggt
     3181 acatcatcgg ggctgaccag aatatctcgg tcaccaagaa caatacgacc gagatcgaca
     3241 tcgtcaacac gctgaagccg ggccggatcc tcgtggtcaa gaagaacacg agcggcaacc
     3301 tgctgcagaa cgcctgcttc acgatcacgc cggacccgaa cagcgcgggc cagaaatgca
     3361 ccaacgcgac cggccaggtc agcttcgaca atctgccgac gacgcagacc tacaagctga
     3421 ccgagaccaa ggcgccgaac gggtacgaga aggccaaggc cgtcacgaac atctcgctga
     3481 cgccgggtct gaccacgacc gtcaacgtga tcgacaagaa ggcgccgcca ccgccgacag
     3541 atggctcggt cctcgtgatc aagttcttct gcccggccgg caaggcgggt gagatgacgc
     3601 agtacctgga ttcgtcggat ccgggtggcg cgaagctcgc ccagacgagt ggctgcacca
     3661 agggcgacgc gaagttcagc ctcgtgccga aggccggagg taccggcatc gagttcaaca
     3721 ccggcgcgga cggccagtac ttcacgacgc tcgccaaggg cacgtgggag ctgcgcgagg
     3781 tctcgccgca gaagagcgat cccgaggacg tgctgatcta cgccggtcag cagacgacgg
     3841 tggtggtgat cgactacgtc aagccgccgg aaccgaagcc ggcgacgatc aacatcctca
     3901 agtacacctg cgcgccgggc ttcggcgggg tctactacgc cgacttcctg aacaactgca
     3961 ccgctgattc gcaactgacc aacaacgtct cgttccggac ctccggaccg accgtggcca
     4021 agcggatcac cggcgcgacc gggcagccgg gacggacatc gttcacgcag ctcaaggccg
     4081 gtgactacac cctgttcgag gacgctccga cgacagcggt ctccgtctac gccttctgcg
     4141 gctacgacgt ctacaactgg gcggactggt actcggctac cggctcgatc ttcctgccgc
     4201 tcaaggaagg cgcgaacgtc acctgcatct ggttcaacgt gccggacgac ttgagcgact
     4261 cgaccggagc gatcgtggtg cacaagtacg tctgcaacgg ccagaacttc ccgtccggat
     4321 tcgactactt cgcgaactgc ccggtcgaga cggacaacgt caagttcgcc atctcgttca
     4381 agcagggcaa caccttcgtg ccgaagacga ccggcatcac caactcgaac gggatcctct
     4441 cgttcggtcg gctcaagccg ggcacgtaca gcctcaagga ggtcggcggc gactggtgta
     4501 acgcgaagtc ggacaatgtg gacgcgcagg gcaacctgat cgttcgcgcc gggcagcgga
     4561 ccaatgtctg gatcttcaac tgcgtgccga ccaagcagcc accgaacacg ggttccggcg
     4621 cgatggccgg ggtgatcggc ggcggcgcgg tcagcagcgc cggggcgacg ctgatcggct
     4681 tcgcctggcc gatcctgggt gtggtcggtt tccggacgcg gaagaagctt cgccgggcag
     4741 cctagcaggt cgcggaaacg gaaccggggt ggtcaatcga ccaccccggt ttcttgtgcg
     4801 gcggatctcg ccttctcacg attttcctat tcccggcagg cacgggcccc ggtaatttca
     4861 cgatgggtcc ctatgcgtgt gttcgcaggg gagtgcattg ctgttctgaa gttgggaggg
     4921 cggtatggat tattcgggtt ggtcaggagt gggcgccttt gcgccgccgg tccagaagcg
     4981 gtttggcggg ttctgggtcc gggtcgttgc ctatttcctg gacgccatct tgctggtgat
     5041 tgcgcaggtg atcctgatgc agatcatcaa gaacgaagcg acgctgcaag gggtgaactt
     5101 cttgctgacc tggttctact tcgccggcct tgagtcgatg gccggggcga ccctcggcaa
     5161 gatggtcctc gggttgtcag tgaccgacga acagggcaac aacatttcgt tcctgcgggc
     5221 gaccggcagg tacttcgcga agatcatctc cgcgctcatc ctctgcatcg gctacatcat
     5281 ggttgcgtgg gatggccgca agcagggcct gcacgacaag atggccggaa cgctggtcgt
     5341 caagaactag cgacagtacg acagcaatag acggccggac ccaaatgatg ggtctggccg
     5401 tcttgcgtgc ccggccggag tggtcgctct ccggccgaac gtcgtgtgct attcgggtgt
     5461 ggccatggcc aggacttgct ctcgagtcat ctccatgtcg tgcacgtctt tcgcgtgctt
     5521 ctggacgacc tcgaccaggg tgtcgtcgtc atccgcgcgg gccacgaaac cgcaatcgca
     5581 ctggaccttc ttcgtcatct gaaccctcct ctccgttgct agactagcaa tagcctacgc
     5641 ctcgcaattt gggaactggt tggcgcgatg ttgggaaaac gttggggccg ctgatccgca
     5701 tctatctggc cggtcaggtc ggaatcgaat gcgacggccg gctcctgtcg gagtggcgtg
     5761 ctgtcggccg gcagggtcgg ctcgcgtttg cgatgctgac ggctgagcat tggcgcgccg
     5821 ttccccgtca ggagctgatc gacgagatct ggccggacga tccgccgccg tcgtctgatc
     5881 gtgccgtgag cgccgtcatg agcaagctgc gggcgctgat ggaacgtgag ggacggccgg
     5941 agatcgagat cgcggcggcg ttcgggtgct atcagatgcg gctgccggcc aacgtctgga
     6001 tcgacatcga agcggcggcc gagggcattg atcaggccga gggattcctg cgcgcgggcg
     6061 acttccgggc tgcctgggcg ccggcccaga tcacctgcca tatcgcccgg cgaccgtttc
     6121 ttcagggcga cgacggcaca tgggcgacac gcactcgggc gaacctgcga gatatgctgg
     6181 tccgggcata cgaggcgttg agcgggatct acgtccggag cggagaaccc tccaacgccg
     6241 ttcgatatgc aaaactcgcg accgaggccg agccataccg tgagacgaca tggcagcacc
     6301 tgatgcacgc ccatgccgtc gctgggaatc gtgcggaagc cctccgttcc tacgagtgtt
     6361 gccggcgctt gctctccgag gagctgggca tctccccctc accgcaaacg gaggcggtat
     6421 atctcgaatt gctgcggagc tgaaccgagg cgctcccggt catgatctct ggtctctcgt
     6481 ctttgttttc cgttttccgt atccattcac caacgaccat caactgctac tttttcgttc
     6541 cctcaaccct caacccccaa ccctccaccc cgtctcgcct acacttcatg catgcaacgc
     6601 gccaatcttt ccgacctcga aacgacgcga ttcgacctga tagtgatcgg ggccggcatt
     6661 aatggagccg cgatcgcccg tgatgcggcg atgcggggtc tgagcgtctg cctgctcgag
     6721 atgaacgatc tcgccagcgg tacctcggcc tggtccagcc ggctcgtcca tggcggtctg
     6781 cgctacctgg agcattacga gctcgggctt gtccgcgagt cgttgcggga gcgggagcga
     6841 ctgctgcgga atgcacccca tctcgtctcg ccgctgccga tggtcgtgcc gatccaggag
     6901 ggcgcgaagc ggggacccct gctgatccgg gccggcatgg tcctctacga catcctgtcg
     6961 tacgacaagt cgctgccgct gcatcggatg ctctcgccgg agaaggcgct cgatcgcgta
     7021 ccggcgctcg atcctgaggg gctaaaagcg gcggccgtct actacgacgc gcaggtgacc
     7081 ttcgccgagc ggctggtggt
//
LOCUS       DATAOZ010000405         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9108927, whole genome shotgun sequence.
ACCESSION   DATAOZ010000405 DATAOZ010000000
VERSION     DATAOZ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9108927"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VHR64_10335"
     CDS             <1..279
                     /locus_tag="VHR64_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3303268.1"
                     /translation="REIVRKVELPLAVPTIMSGVRTATINIVATATIAPLAGVLTLGD
                     FIINRNVYGDDGVLAGAILVALLAVTLELILAGVQYMLTSPGLRPRKA"
     gene            317..1297
                     /locus_tag="VHR64_10340"
     CDS             317..1297
                     /locus_tag="VHR64_10340"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3303269.1"
                     /translation="MSKQTRLHAVLTAIAVLALALGIAACGGGDDDNGTESGASGKLI
                     QSNPANGKVTITVGSKNFTEEFILGEIYAQALQHAGYNIKKDLNLGDSTIAIKALKQG
                     EISGYPDYISTLLTEFYGLQPNQIPPNSQQGAAKTQSELQKDGLTSFAPTPFADANAL
                     GTLKSTADKLGVTTISELQGKSQDLTLYGSPECRQRIDCLVGLEDIYGLKFKSFTPVD
                     IGLRYTVLDKGQADLSILFTSDAQLAAQPDKYVLLKDDKGIFPAGNVVFITDPATVKK
                     AGPDYQKTIEQVQRGLSLPVIQELNARVDIDKQTPADVAAEYLKESGYIK"
BASE COUNT          301 a          495 c          485 g          250 t
ORIGIN      
        1 cgtgagattg ttcgcaaggt ggagttgccg ctggccgttc cgacgatcat gagcggtgtc
       61 cggacggcga cgatcaacat cgtggccacg gccacgatcg cgccgctcgc cggggtcctc
      121 accctgggcg acttcatcat caaccgcaac gtctacggcg acgacggagt gctggcgggc
      181 gcgatcctgg tcgcgctgct ggctgtcacc ctggagctga tcctcgccgg agtgcagtac
      241 atgcttacgt ctcccggcct ccgtccacgg aaggcctgat gctctcgtcc cggaaacgac
      301 gaaggaggaa ccagtcatga gcaagcagac gcggcttcat gccgtgttga ccgcgatcgc
      361 ggtgttggcg ctggcgctcg gaatcgccgc ctgtggcggc ggtgacgacg acaacggcac
      421 tgaaagcggt gccagtggga agctgatcca atccaacccc gcgaacggaa aggtcacgat
      481 cacggtcggt tcgaagaact tcactgagga gttcatcctc ggtgagatct acgcccaggc
      541 gctgcagcat gcgggataca acatcaagaa ggacctcaac ctgggcgact ccacgatcgc
      601 gatcaaggca ctcaagcagg gtgagatctc cggatatccg gactacatca gcaccctgct
      661 gacggagttc tacggcctgc aaccgaacca gataccgccc aactcgcagc agggcgccgc
      721 caagacgcag tcggagctcc agaaggacgg cctgacctca tttgcgccga ccccgtttgc
      781 ggacgcgaac gcgctcggga ccctgaagag cactgccgac aagctcgggg tgacgaccat
      841 ctccgaactg cagggcaagt cgcaggacct caccctgtac ggctcgcccg agtgccggca
      901 gcggatcgac tgcctggtcg ggctggagga catctacggc ttgaagttca aatcgttcac
      961 cccggtcgac atcgggctgc gctacacggt gctggacaag ggccaggcgg acctctcgat
     1021 cctgttcacg agtgacgcac agcttgccgc gcagccggac aagtacgtgc tgctcaagga
     1081 cgacaagggg atcttcccgg ccggaaacgt cgtcttcatc accgatccgg cgactgtcaa
     1141 gaaggccggt ccggactatc agaagacgat cgagcaggtc cagcgtggcc tctcgctccc
     1201 ggttatccag gagctgaacg cccgagtgga catcgacaag cagacgccgg cggatgtcgc
     1261 cgccgagtac ctgaaggagt ccggctacat caagtagccg actgcctctc tcccgccgcc
     1321 ctgcatctcg cggggcggcg ggacgcgggg gtctaagaca atcgtcacgt gattgccggt
     1381 ccggccaggc aaggtttacc cgggctcagc tgagggaaga gcccatggtg ggagacgccg
     1441 atctccgaga ggaggctcgg atgtccaccc cacagacgac ccgtctgggc gtcgggtctt
     1501 cccccttccc ggcgtccggc gggtcctctg c
//
LOCUS       DATAOZ010000406        12608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8848940, whole genome shotgun sequence.
ACCESSION   DATAOZ010000406 DATAOZ010000000
VERSION     DATAOZ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12608
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8848940"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VHR64_10345"
     CDS             complement(<1..297)
                     /locus_tag="VHR64_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX3303270.1"
                     /translation="MTNFDRFTERAKVSLQLAQEEARRFRHDHLGTEHLLLGLVREGD
                     GIAAQALNASGVDLPKLRAGIEFVVGRGDSDLSEIGLTPRSKKALEIAIEESRRL"
     gene            complement(482..3010)
                     /locus_tag="VHR64_10350"
     CDS             complement(482..3010)
                     /locus_tag="VHR64_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3303271.1"
                     /translation="MADARDAHTQIKQPDVAYDRPTGQGDPVDAERRGTRVATRVLVA
                     AVSTVALAGWLAALLSPGSTTSDQYLTAAIIFVFAVGSACVPLQFAYHQEMTFELTIL
                     VLGALLLPVYLGITVGVLGYGLGYAIRYRGQFGLDCPFNAGAFALSLAATALAFDALG
                     WAPMVHEASLARNVAGGLMAAAIWLIITRVLVSLVVSLESRQAFRSVLRDSTLGMQSA
                     EQAMLAAMVALGILGAVVARAHPLALLLLLVPAFALWLALRQNVETRHRIEASLATAQ
                     RVAGIGSLDWDLKQGDIRWSDILFQILGYQPRAVESTVDSYVKRVHPADRNRVMAAFQ
                     AAAEGRQVAIEHRVALGTGEERAFDLKFNGVPGRRGVVKRTVGTVHDVTDRKQLEERL
                     HHQAYHDALTGLPNRAMFMQRLDQAFGRQHRGRAIALLFLDLDRFKLINDTLGHDAGD
                     QLLRIVGKRLEGCIRPNDVVARLGGDEFTILLDNVHGDADAEVVAERIIAAINQPMTL
                     LGTRDVVISTSIGIVRPGPEQESGADLMRDADTALYRAKELGRNRYALFDASMGEETR
                     ERLALEEDLRSAIERRQMSLVYQPRIDLASGRVVMVEALVRWTHPTRGVIPPTRFIPI
                     AEETGQIEPLGRWILETAVAEAATWGRVLNPSPTLSVNVTSKQLHDPEFGAWLGVLIG
                     ETGLGGSQIRLEVPESVVMKNVESAIGAIGALQHIGVRVAIDDFGTGHSSLASLRRFP
                     IDTLQLDRQFVTEIGANREATTVAQAVIGLAHGLGLRVVAAGVERQEQAEQLTAMGCE
                     LAQGNFFCPPVPGPELLAFLIRNQQTTTEQRRAIDPPNIRRITG"
     gene            complement(3141..3284)
                     /locus_tag="VHR64_10355"
     CDS             complement(3141..3284)
                     /locus_tag="VHR64_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303272.1"
                     /translation="MGTRRLTRWVRVGLVAIALAGLTAVPMIDSASAATFTGRDNWCQ
                     RCY"
     gene            complement(3884..6307)
                     /locus_tag="VHR64_10360"
     CDS             complement(3884..6307)
                     /locus_tag="VHR64_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEX3303273.1"
                     /translation="MARSIVTLIAVMGVMLAALGWYTPQPARAQTASLLINNHLCPNP
                     ISSIDMIDLAPNCPGVGSNWSFTVTHTGGLYQQTLQTDASGMASFGSMPAGVYRIDGA
                     TTPDFSKVAVYCNVKDGLGNDVTGWLYGYADNYYTELNIAQDGYMGYCDWWWYNPGAT
                     PQPTTGSVMINKIDCPPGYDVTNVSIYDLAATCHGQGQYEFSLTDANAGNQSGTTPGG
                     GLNTVSFSNVPSGPMLITEDAPGDYLQPRVFCKNQKLTGEEDPEDEVPVNNFAINPTL
                     KQGYDSLYCDWFNVPSPDKVNITITKYECPDGFVSSDPSQLTSTCTEPYNPVTFKLDG
                     ASSGNPGDQDTGTVIANGVQWTNMDADTWYITEFLPQGHNQPIIFCSVTDHANNSQTG
                     PQQQSPEQVQDGYRLTYTLDGGTALSCDWFNTKGTPTTTTGTISIHKYGCPEGYDTSW
                     DLQQWSQFCTTIVQGASFTVSSGSTSDEQQLTGIDLTWEQLAPGDYTVTETQPSAWSG
                     SVVYCASGTGDTLGSYESADVTDNGISDTLESGELLDCYWYNLAKPRPVATIDPNAPA
                     TLTIVKFTCKEDYDPLAQDAKPAKDCEEKTDDVTFSIIGAKNASISGKTGDDGEGTVT
                     FKNLKPGSYLLQETYPEHTQKAFIWSCESSTRVHDYPFAPFARIDETGMIKVSLTPGE
                     TLKCDWYNVPSPPEDEATPSAAGGDVAVTIDVFMCPTHAVSPASCDPVEAGVGVSLTS
                     TSGNGEPIDLETDDTGVATGSVPADEYDVDADEMICFAESPAFTSTGTLDLTSEDSAD
                     VQIYFCGQH"
     gene            complement(6782..7978)
                     /locus_tag="VHR64_10365"
     CDS             complement(6782..7978)
                     /locus_tag="VHR64_10365"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX3303274.1"
                     /translation="MATVPPVPAAIARVGVGAHETWEVALDEAAAGLGEVAPDLVLIG
                     VGSAFVSEVPAIADQVWRTFQAPIVLGASGRGVIAQHSEYEHASTVALMGLRLPGAIL
                     SPAHLTQRTLEHLTDPATCYRRLGAIPEDINGWIVLANPFRFDILSALAALEMAYPGA
                     PVVGGVASPDAQTRQTALLINGEAVFDGAIALGIGGPYELLPAVSHGCEPIGQPWTIT
                     GVSGDWIESIGGRPALAILDDILQRTPQDLRNQTRTNLLVGLATDEYRTEFQRGDFVV
                     RSIAGVDQPSGAIAIGARARPGQTIQFQLRDPAVADLDLTFSLDGLRGSLGELDPVAM
                     VAFAGQERGRNLFGSESHDTLTIQRKFPGIPTIGLYTAGEIGPAGPASAVHSLSLTLG
                     LIARRH"
     gene            complement(7988..8755)
                     /locus_tag="VHR64_10370"
     CDS             complement(7988..8755)
                     /locus_tag="VHR64_10370"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX3303275.1"
                     /translation="MATIGELTDFSVADLMTVISQRRRTGRLMIKTGGNEAGLFFEDG
                     LLVRVASGDIALRIGRMLIRQNLIDTPRLLEALHMQAESGEDTPIGEVLLRKGWITQA
                     DLHRCLEEQSIEVLTRAMSSHAGVFTFDAGVRVSRSGDFPPMEPIGLLKIAVERTSAI
                     SVLKERLPSTVTPFFLNVSPAMVVDLQLTLEPAEGIALGILRNGPLTFPELANMTALD
                     ELTLGAAILTLLENEYIATASQLLPSPRPQTPAARAS"
     gene            9533..11485
                     /locus_tag="VHR64_10375"
     CDS             9533..11485
                     /locus_tag="VHR64_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX3303276.1"
                     /translation="MRDDLIATSSPWLERTLAHRSATAAPWIAVSTAMGLALTGIGRF
                     FYAGQAPAEQWFLVPAVLLSLVAVGLIALQLRLESRAGRAAEERVADVEHRFAQILGT
                     ASDLAAIVDARGSLTYLSPSAERLLGRPARSLVGQSLATLAIPADQFHAGWLVASARA
                     AQESPRIRLRLATALGEERIVEAQAASQLADPRIEGIVINARDITEQVRLEQEREDIQ
                     TRDLLTGLMNRTAFVAQLDEAVLRAGRLRRSLCLAVIDLDHFSVVNDQIGEEGGDAVL
                     RIVADRLTSVAGPGATLGRLAGDRFGMLLTDSGLTQAAATFEGARDALSRPVLVGERT
                     VPIGASIGVTSRGDGQIGTTDALISEAEAALLAAQQAGPGQLIIFAPNMAIPDGRLDL
                     EADLRAAVERGEFVVFYQPVVSLDAGQVVEMEALIRWNHPARGLVSPGEFIPVAEETG
                     LIVPIGWWVLEEACRQGMRWQRHGGRSLIMSVNLSPRMFRQGDLVHRVRTILAETGFP
                     ADRLRLEITEGVMIEDCMQAAAMLQTLKEMGVHLAIDDFGTGYSSLAYLQHFPVDVIK
                     IDRSFVATMDECRESNEIIRSIVDLAKRLEIETTGEGIETADQLAQLRELGSDRGQGY
                     LFSRPLPAADLDRILSQSMKLGTYAA"
     gene            11675..12199
                     /locus_tag="VHR64_10380"
     CDS             11675..12199
                     /locus_tag="VHR64_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX3303277.1"
                     /translation="MHKEWPHRQSHRLRGYNYAGPGTYFVTVCTADRCQFFGVVRRKT
                     MFLSDWGEIAAREWPVSLARRPYIIPHAFIVMPNHVHGLISFDPATTSDRDLESMSRG
                     MPPQSLGRFLNGFKGAVTTIIRGKIDDPFFEVWQSNYHDRVVRNEREFDSFHAYILDN
                     PARWEEDRFNNERM"
     gene            complement(12365..>12608)
                     /locus_tag="VHR64_10385"
     CDS             complement(12365..>12608)
                     /locus_tag="VHR64_10385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3303278.1"
                     /translation="IAPGLGGCEIIETRVGLRPWSPDGVPAIGRLTGTDNGWICTGHG
                     PSGLTIGPYSAELLAQLITNEDPGLDPTPYSPDRWQ"
BASE COUNT         2148 a         4083 c         4170 g         2207 t
ORIGIN      
        1 caggcggcgg ctctcttcga tcgcaatctc tagtgccttc ttggatcgcg gagtcagccc
       61 gatctcactt agatctgaat ctccccggcc gacgacgaac tcgataccgg cgcggagctt
      121 tggtaagtcg acaccggatg cgttcagagc ctgcgcggcg atgccatctc cctcgcgcac
      181 caggccaagc aagaggtgct ctgtcccgag gtgatcatgc ctgaatcgcc gcgcttcctc
      241 ctgggcaagc tgcaacgata ccttggcacg ttccgtaaac cggtcgaaat tggtcatgct
      301 gctgtccttg ccggcacccg cagcgtacca cgcaccgagc catgcccacc ccgcagaagc
      361 aacccggggt aaccaggtac accgcggatg gagcctccgg gacacgtgat tttgaccatc
      421 aaacccgccg gggaaccgag tcgaggaggc acaaccggct ctgaaaacgg gccctcctga
      481 ctcaaccagt gatgcggcgg atgttgggtg ggtcgatcgc gcgacgctgc tcggtggtgg
      541 tctgctggtt gcggatcagg aaggccagca gctccgggcc gggcaccggc gggcagaaga
      601 agttgccctg ggccagctcg cagcccatcg cagtcaattg ctcggcctgc tcctgccgct
      661 ccaccccggc cgcgaccacc cgcaggccca agccgtgggc gagaccgatc accgcctgcg
      721 caaccgttgt cgcctcccgg ttcgccccga tctcggtcac gaactggcgg tcgagctgga
      781 gggtgtcaat cgggaagcgc cgcagcgagg cgagactgga gtgcccggtg ccgaaatcgt
      841 cgatcgccac ccgcacgccg atatgctgga gcgcgccgat cgccccgatc gcgctctcga
      901 cgttcttcat gacgacgctc tcgggcacct cgaggcggat ctggctgccg ccgagcccgg
      961 tctcgccgat cagcacgccg agccacgccc caaactccgg gtcatggagc tgcttgctgg
     1021 tcacattgac gctcagcgtc ggcgatgggt tgagcacccg gccccaggtc gccgcttccg
     1081 cgaccgctgt ctccaggatc cagcggccga gcggctcgat ctgcccggtc tcctccgcga
     1141 tcgggatgaa tcgggtcggc ggaatcacgc cacgggtcgg atgcgtccac cgcaccagtg
     1201 cctcgaccat cacgacccgg cccgaggcga gatcaatccg gggctggtag acgagcgaca
     1261 tctgccggcg ctcgatcgcg ctgcgcaaat cctcctcgag cgccagccgc tcccgcgtct
     1321 cctcgcccat cgaggcgtcg aagagggcat agcggttccg gccgagctcc ttggcccggt
     1381 aaagcgcggt atcggcatcg cgcatcaggt cggcgccgga ttcctgctcc ggaccaggcc
     1441 ggacgatgcc gatgctggtc gagatgacca cgtcccgcgt gccaagcagg gtcatcggct
     1501 ggttgatcgc ggcgatgatc cgctccgcca cgacctccgc atcggcatcg ccatggacgt
     1561 tgtcgagcag gatcgtgaac tcgtcgcctc ccagccgggc gacgacgtcg ttcggccgga
     1621 tgcagccctc gagtcgcttg ccgactatac ggagaagttg gtcgcccgcg tcatggccga
     1681 gggtgtcgtt gatcagcttg aagcgatcga gatcgaggaa gagcaaggcg atcgctcggc
     1741 cgcggtgctg gcggccgaac gcctggtcga gccgctgcat gaacatggcc cggttcggca
     1801 gaccggtcaa cgcatcgtga tacgcctggt gatggagccg ctcctcgagc tgcttgcgat
     1861 cggtcacgtc gtggactgtc ccgacggtcc gcttgacgac accgcgccgt ccgggcacgc
     1921 cgttgaactt gaggtcgaag gcccgctcct cgccggtgcc gagcgcgacc cggtgctcaa
     1981 tcgcgacctg ccgaccctcg gctgcggcct ggaaggcggc catcacgcgg ttgcggtcgg
     2041 ccgggtggac gcgtttgaca taggaatcga cggtcgactc gaccgcgcgc ggctggtagc
     2101 cgaggatctg gaagaggatg tcggaccagc ggatatcgcc ctgcttgagg tcccagtcga
     2161 gcgagccgat cccggccacg cgctgggcgg tggcgaggct ggcctcgatc cggtgccgcg
     2221 tttccacgtt ctggcgcaac gcgagccaga gcgcgaacgc cggcacgagc agaagcagca
     2281 gggcgagcgg atgagcccgg gcgacgaccg cgccgaggat gccgagcgcg accatcgcgg
     2341 ccagcatcgc ctgttcagcc gattgcatgc cgagcgtgct gtcgcgcagc accgaccgga
     2401 acgcctgccg gctctccagc gaaacgacga gcgagaccag aacccgggtg atgatcagcc
     2461 agatcgcggc ggccatcagt ccgcccgcga cgttccgtgc caggcttgcc tcgtggacca
     2521 tcggcgccca gccgagcgca tcgaacgcga gtgcagtggc ggcaagactg agcgcaaacg
     2581 caccagcatt aaaggggcag tcgagtccga actgtccgcg gtagcggatc gcgtagccga
     2641 gtccgtaacc aaggacgcca accgtgatcc cgagatagac aggaaggagc aaagcgccga
     2701 gcaccaggat agtcagctcg aacgtcattt cctggtgata cgcaaattgc agaggtacgc
     2761 acgcggagcc taccgcaaag acgaagatga ttgctgcagt caggtactgg tcgctggtgg
     2821 tcgaacccgg ggagagtagc gcggcgagcc aaccggccag agccacggtc gatacagccg
     2881 ccacgagcac ccgcgtggca accctggtcc ccctgcgctc ggcgtcaacg gggtctccct
     2941 gacctgtggg tcggtcatag gcgacgtcag gttgcttgat ctgcgtgtgc gcatcacgcg
     3001 cgtcggccat ctggatgtcc tgacgcgggg tcttgaaagc gaaacggcct ccggggctga
     3061 tgtcgccatc atagttcccc ggaggccgaa ttgcctatga cttgtgtctt agccggcgcc
     3121 catccgcgcc gcccgcgtgc ctagtagcag cgctgacacc agttgtcgcg gccggtgaag
     3181 gtagcggcag atgcgctgtc gatcatcggc acggcggtca gccccgccag cgcgatcgcg
     3241 accagcccga cgcgaaccca ccgagtcaat ctgcgcgtgc ccattctgtt cccccgagtg
     3301 cgatgtcatt cgcaatcttg ccgatcgcga actgagcagt aaccgcacga cgcgcaccaa
     3361 atctgcgcaa attgcggcta cttcgagggt aggcgggccg attgtctcgg gccatggccg
     3421 atcgggttga gaaagatggg gatacgtacg tactgtgacc acccgaacgg gggatatcag
     3481 aagggggctg aacgaacacc cgatcccggt gctcccgggc tacgcggcgc acaaatccct
     3541 ctcccgttca cgggaaaggg caggggtgag ggccggtgcc gggagcgcct gttcgcagca
     3601 ccaatcgcga gtcccggggc cgccccgagc tgaaggtcgg ggctgagcat acacaagccc
     3661 actgaagggc ctggaacgat gctgcgataa accgcccagc cccttcagtg ggctttccat
     3721 gacttagccc gggcatttat ccccgggggg tgccgagcgt ccggtacgta taccgcgttc
     3781 cggcctgaac gacggggcgg ctccagtcac ggcgcgggcg gattcatggc cctcatcccc
     3841 ggacccctct cccgcgtgcg ggagagggga ggagatttgg gggttagtgc tgcccgcaga
     3901 agtagatctg gacgtcggcg gaatcctcgc tcgtcaggtc gagcgtgccg gtcgacgtga
     3961 aggccggtga ttccgcgaag cagatcatct catcggcatc cacgtcgtac tcatcggccg
     4021 gcaccgagcc ggtcgcgacg ccggtgtcgt ccgtctcgag gtcaatcggt tccccgttgc
     4081 cgctggtcga ggtcagtgac acacccacgc cggcctcaac cgggtcgcac gaggcgggac
     4141 tgaccgcgtg cgtcgggcac atgaagacat cgatcgtgac cgcgacgtcg ccaccggcgg
     4201 ccgacggggt cgcctcgtcc tccggcgggg aggggacgtt gtaccagtcg cacttcaggg
     4261 tctctcccgg agtcaggctg accttgatca tgccggtctc atcgatccgg gcgaagggcg
     4321 cgaacggata gtcgtggacg cgcgtcgagc tctcgcagct ccagatgaag gccttctgcg
     4381 tgtgctccgg gtaggtctcc tggagcaggt agctcccggg cttcaggttt ttgaaggtga
     4441 ccgtgccctc gccatcgtcg ccggtcttgc cactgatgct ggcgttcttg gcgccgatga
     4501 tcgagaaggt gacgtcgtcc gtcttctctt cgcagtcctt ggcaggcttg gcatcctgcg
     4561 cgagcggatc gtagtcctcc ttgcaggtga acttgacgat cgtcagggtc gccggcgcgt
     4621 tcggatcgat cgtagcgacc gggcgcggct tggccaggtt gtaccagtag cagtcgagca
     4681 gctcgccgct ctcgagcgtg tcggagatgc cgttatcggt gacatccgcg gattcatagc
     4741 tgccgagcgt gtcgccggtg ccgcttgcgc agtagacgac gctcccactc caggcgctcg
     4801 gctgcgtctc cgtgaccgta tagtcgcccg gagcgagctg ctcccaggtc aggtcgatgc
     4861 ccgtcaactg ctgctcgtcg cttgtgctgc cggacgagac cgtgaagctc gctccctgga
     4921 cgatcgtcgt gcagaactgg ctccactgct gcaggtccca gctcgtgtcg tagccctcgg
     4981 ggcatccgta cttgtggatc gagatggtgc cggtcgtcgt ggttggcgtg cccttcgtgt
     5041 tgaaccagtc gcaggagagc gccgttccac cgtccagcgt gtaggtcagc cggtagccgt
     5101 cctgcacctg ctcgggcgac tgctgctgcg gaccggtctg cgagttgttc gcatggtcgg
     5161 tcaccgagca gaagatgatc ggctggttgt gcccctgcgg caggaactcg gtgatgtacc
     5221 aagtgtcagc gtccatgttg gtccactgca cgccgttcgc gatgacggtg ccggtgtcct
     5281 ggtcaccggg attgccggag gacgcgccat ccagcttgaa ggtgaccggg ttgtagggct
     5341 cggtacaggt gctggtcaac tggctcgggt cggacgatac aaacccatcc gggcactcgt
     5401 acttggtgat cgtgatgttg accttgtccg ggctggggac attgaaccag tcgcagtaga
     5461 ggctgtcgta cccctgcttg agcgtcggat tgatcgcgaa gttattgacc gggacctcgt
     5521 cctccgggtc ctcctcgccg gtcagcttct ggttcttgca gaagacgcgt ggctgcaggt
     5581 aatcgcccgg cgcgtcttcg gtgatgagca tcggtccgga gggcacattg ctgaagctga
     5641 cggtattcag gccgccgccg ggcgtcgtgc cagactggtt gccggcgttg gcgtcggtca
     5701 gggagaactc gtactggccc tgcccgtgac aggtggcggc aaggtcatag atcgagacat
     5761 tcgtgacgtc ataaccgggg gggcaatcga ttttgttgat catcaccgag ccggtggtcg
     5821 gctgcggcgt ggcgccgggg ttgtaccacc accagtcgca gtagcccatg tagccgtcct
     5881 gcgcgatgtt gagctcggtg tagtagttgt cggcgtatcc gtacaaccag ccggtcacgt
     5941 cattgccgag gccgtccttg acgttgcaat agacggcgac cttagagaag tcgggcgtcg
     6001 tcgcgccgtc aattcggtag acgccggcgg gcatgctgcc gaaggaggcc atgccgctgg
     6061 catcggtctg gagcgtctgc tggtacaggc cgccggtgtg ggtgacggtg aaggaccagt
     6121 tcgaaccgac gccggggcaa ttcggcgcca aatcgatcat gtcgatgctc gagatgggat
     6181 tgggacagag atggttgttg atcagcaatg atgccgtttg ggcgcgggcg ggttggggcg
     6241 tgtaccagcc cagggcggcg agcataaccc ccatcaccgc gatcagcgtc acaatcgaac
     6301 gtgccaaacg acggcgcctg aacgaatgca taggtgcaga ctcctcgcga atgcgacaga
     6361 tccggatttc gggggttaat tggcaagcag cataccacta atcagcgcaa acgcgaccca
     6421 tccattcggc ctacccgctg gggcgctgcg cgaacgtccg ccttgatgct gtccgaaact
     6481 tgcggattta ctcgcgttct cgcggagact agtcgcccgt gccgcccggg gataaatgcc
     6541 cgggctgaga aacgagaagc ccactgaagg ggctatggaa tggacgggga gtcaacaagg
     6601 ccccttacct agtcccttta gtggactcat tgcgatttag cccggacctt tagctccggg
     6661 cggtgccgga cggcgggttt ccgcgagaag aggggttgat ccccagcaat gctcggcgtc
     6721 gcagcactga ccttcaatcc ggggtggctc cgagcaccgg ctcctgccga gccactcagc
     6781 gtcaatgacg gcgggcgatc aggccgaggg tcaggctgag ggaatggacc gcgctcgccg
     6841 gtccggccgg gccgatctcg ccggcggtgt agagaccgat ggtcgggatg cccgggaact
     6901 tgcgctggat cgtcaacgtg tcgtgactct cggagccgaa caggttccga ccccgctctt
     6961 gccccgcgaa ggccaccatc gcgaccgggt ccagctcgcc gaggctgccg cgcaggccat
     7021 cgaggctgaa ggtcagatcc agatcggcga ccgccgggtc ccgcaactgg aactggatcg
     7081 tctgccccgg ccgggctcgc gcgccaatcg cgattgcgcc tgacggctga tccaccccgg
     7141 cgatcgagcg gacgacgaag tcgccgcgct ggaactcggt ccggtactcg tcggtggcga
     7201 ggccgaccag caggttggtg cgcgtctggt tgcgcaaatc ctggggcgtg cgctggagga
     7261 tatcgtccag aatcgccagg gcgggtcgtc caccgatcga ctcgatccag tcacccgaga
     7321 cgccggtaat cgtccagggt tgcccgatcg gctcgcagcc gtgcgagacc gccggcagca
     7381 actcatacgg tccgccgatg ccaagcgcga tcgcgccatc gaagacggcc tctccattaa
     7441 taaggagggc cgtctgccgc gtctgggcat cgggcgaggc aacgccgccg acaaccggag
     7501 cgcccgggta ggccatctcg agcgccgcca gggcggagag gatgtcgaac cggaatgggt
     7561 tggccagcac gatccagccg ttgatgtcct cggggatcgc accgagccgc cggtaacagg
     7621 tggctgggtc ggtcagatgc tcgagcgtgc gctgggtgag atgtgccggc gagaggatcg
     7681 cgccaggcag ccgcaaaccc atcagcgcga ccgtcgacgc gtgctcgtac tccgaatgct
     7741 gcgcgatcac gccacgcccg ctcgcgccga gcacgatcgg cgcctggaat gtgcgccaga
     7801 cctgatcggc gatggccggc acctcgctca cgaaggcgct gccgaccccg atcaggacga
     7861 gatcgggcgc gacttcgcca agcccggcag ccgcctcgtc gagggcgacc tcccaggttt
     7921 cgtgggcgcc gacgccgacc cgcgcgatgg cggccggcac tggtgggact gttgccattg
     7981 gctcgtgcta gctggctcgg gcggcaggtg tctgcggtcg cgggctcggc agaagttggc
     8041 tcgccgtggc gatgtactcg ttctcgagca gcgtcaggat cgccgcgccg agcgtgagct
     8101 catcgagtgc ggtcatgttg gccagctccg ggaaggtgag cgggccattg cggagaatcc
     8161 cgagcgcaat cccttcggcc ggctccagcg tgagttgcaa atcgaccacc atcgccgggc
     8221 tgacgttgag gaagaagggc gtgaccgtac ttggcagccg ctccttgagc accgatatag
     8281 cgcttgtgcg ctcgaccgcg atcttgagca gcccgatcgg ctccatcggc gggaagtcgc
     8341 ccgagcggct gacccgcacc ccggcatcga acgtgaagac gcccgcgtgc gagctcatcg
     8401 cgcgggtcag cacctcgatc gactgctcct cgagacagcg gtgcaaatcc gcttgagtaa
     8461 tccagccctt gcgcagcagc acctcaccga tgggcgtgtc ttcacccgat tcggcttgca
     8521 tgtgcagcgc ctcgagcagg cggggtgtat cgatcaggtt ctggcggatc aacattcggc
     8581 cgatccggag agcgatgtcg ccgctcgcga cgcggaccag caggccgtcc tcgaagaaga
     8641 gtcccgcctc gttcccgccg gtcttgatca tcagccggcc ggtgcgcctg cgctggctga
     8701 tgaccgtcat gaggtcagcc acactgaaat cggtaagttc gccaatcgtc gccacgaaga
     8761 gtctccgccc aagatggtca ggcagcgccg aactctgcgg gctgccgatg tgaccaccgt
     8821 agagcgcgag acgtccgtac ggtatcgcga gatagtcctg tttatgaagg caagcagtct
     8881 ggagagttga gggtggaggg tggagggaac gaaaggggga acggctgtag cgtcgcatgg
     8941 cactgcgatt tgggctagcg gtagagcggg gcgatgcatg gagaccggca ttccggcggc
     9001 gcccggggat aaatcccagg gctacatcca ataagccagc tgaagcaggc tggatggagc
     9061 cagtaccggc gcgtaacgca gagttaccac gcctcgcttg gtgcatcccc acgcgcggtt
     9121 gatggggccg agccattgaa gaggttggtt tccccacgtg atggccaaca tcgggtccgg
     9181 gcggaacgtg ttaccacgct ccgtcttcgg attcggcgct tcgcaccgtt cgcccgctag
     9241 cccaaatcgc agtgccatgc gacgctacag ccgttccttc tccaccaact atccaccgtc
     9301 caccatcaac cccctttcgt tccctccacc ctcaaccatt aacccttaac ccgtctgtac
     9361 gtacaccaag atttgtgcaa taatcgggcc tgcgagtcag gactaacggc ctagtacctt
     9421 ccgccccatc ggcctgaccc gatctggtag ggtgcgaggt gcacccacaa ccggaccgca
     9481 ccggttcccg tcgcgcgcgc caggacgatc tggcgctgag gaggaccacc acgtgcggga
     9541 cgacttgatc gcgacctcat caccatggct cgagcggacc ctcgcgcacc gaagcgcaac
     9601 cgccgcgccc tggatcgccg tctcaaccgc gatgggcctc gccctgaccg gtatcggccg
     9661 cttcttctac gccggacagg cgccggccga gcagtggttc ctggtcccgg ccgtgctgct
     9721 cagcctggtt gcggtcggat tgatcgcgct tcagctccga ctcgagtccc gggccggccg
     9781 cgccgccgag gagcgtgtcg ccgacgtcga gcatcgcttc gcccagatcc tcggcaccgc
     9841 cagcgacctg gctgcgatcg tcgacgcgcg cggctcgctc acctatctca gcccgtccgc
     9901 cgagcgcttg cttggccgcc cggccagatc gctggtcggc cagtcgctcg ccacgcttgc
     9961 gattccggcc gatcagtttc acgccggttg gctggttgcc tcggcccggg cggcgcagga
    10021 gtcgccccgg atccggctgc ggctggcgac cgcgctcggc gaggagcgga ttgtcgaggc
    10081 gcaggccgcc agccagctcg ccgatccgcg catcgagggc atcgtcatca acgcgcgcga
    10141 catcaccgag caggtccggc tcgaacagga gcgcgaggac atccagaccc gcgacctcct
    10201 gaccgggctg atgaaccgga ccgccttcgt agcccaactg gacgaggccg tgctccgcgc
    10261 cggtcgactg cgccggtcgc tctgccttgc cgtgatcgat ctcgaccact tcagcgtcgt
    10321 caatgaccag atcggcgagg agggcggcga tgccgtgctc cggatcgtgg ccgaccgcct
    10381 gacctcggtt gccgggccgg gcgcgacgct cgggcgattg gccggcgacc gcttcggcat
    10441 gctgctgacc gattccggcc tgactcaggc ggccgcgact ttcgagggcg cccgcgatgc
    10501 cctttctcgc ccggtcctgg tcggagagcg gacggtcccg atcggagcca gtatcggcgt
    10561 gaccagccgc ggcgacggcc agatcggcac gaccgacgcc ttgatcagcg aggccgaagc
    10621 ggcgctcctg gccgcgcagc aggccggtcc gggccaactg atcatcttcg cgcccaatat
    10681 ggccatcccg gatgggcggc tcgacctgga ggccgacctg cgcgcagcgg tcgagcgcgg
    10741 cgagttcgtc gtcttctacc agcccgtcgt ctcgctcgat gccggtcagg ttgtcgagat
    10801 ggaggcattg atccgctgga accacccggc gcgcggcctg gtctcgccgg gcgagttcat
    10861 tccggtggcg gaggagaccg gcctgatcgt gccgatcggc tggtgggtgc tcgaggaggc
    10921 ctgccggcag ggcatgcgct ggcaacgcca cggcggccgc tcgctgatca tgagcgtcaa
    10981 tctctcaccg cgtatgttcc ggcaaggcga tctggttcac cgcgtccgca cgatcctggc
    11041 cgagaccggc ttcccggccg accggctccg gctcgaaatc accgaaggcg tcatgatcga
    11101 agactgcatg caggcagcag ccatgctcca gacgctcaag gagatgggcg tgcatctggc
    11161 gatcgacgac ttcggcaccg gctactcgtc gctggcctat ctgcagcact tcccggtcga
    11221 cgtgatcaag atcgatcgct cattcgtcgc gacgatggat gagtgccgcg aaagcaacga
    11281 gatcatccgc tcgatcgtcg acctggccaa gcggctggag atcgagacga ccggcgaggg
    11341 aatcgaaacg gccgatcaat tggcgcagtt acgggaactc gggtccgatc gcgggcaggg
    11401 ttacctcttc tcgcgcccgc tcccggccgc cgacctcgac cgcatcctca gccaatccat
    11461 gaaactcggc acctacgccg cataagcgag tggaggggtg agagtcgagg ggggagggtt
    11521 aacgaattcg tcttgtaggg agttgccgtg gcatatccgc gcggttccgg gcagcatttc
    11581 acgtcagggg aacgggtccg gcaccaagtg gcagccacct cggcaacggg cggatttgcc
    11641 acggcaaatc cctacattca gtatgctgcc gaatgtgcac aaggaatggc cgcaccggca
    11701 atcacatcgg ttgcggggat acaactacgc tggtccgggc acctacttcg tcaccgtctg
    11761 caccgctgat cgctgccagt tttttggcgt ggtgcgacga aagacgatgt tcctttcaga
    11821 ttggggcgaa atcgccgcgc gggaatggcc ggtctccctc gcccgtcgac catacatcat
    11881 tccgcacgcc ttcatcgtca tgccgaacca tgtgcacgga ttgatctcct tcgacccggc
    11941 cacgacctcg gatcgggatc tggagtcgat gtcgcgcggg atgcctccac agtctttggg
    12001 ccgatttctc aacggattca agggcgccgt cacgaccatt atccggggca agatcgatga
    12061 cccgttcttc gaggtgtggc agtccaatta tcacgaccga gtcgtgcgca atgaacgcga
    12121 gttcgactcg ttccacgcct acatcctcga caacccggca cgctgggaag aggaccgctt
    12181 caacaacgaa cgcatgtagg gatttgccgt ggcaaatccg cgcgctgcca aaccgcccgc
    12241 tgccagttgg tggatagctg attggcgtca ggcagggggt cggggtccat tgcgggtttg
    12301 ccacggaaga caccaatatg cgggttctcg gtctttcgct ttccgttccc tcaaccagtc
    12361 aaagctactg ccagcggtcc ggggagtacg gagttgggtc gaggcctggg tcttcgttgg
    12421 tgatgagctg ggccaacaac tcggccgagt aagggccgat ggtcaggccg ctggggccgt
    12481 gtccggtgca gatccagccg ttgtcggtcc cggtcagcct cccgatcgcc gggacgccgt
    12541 ccgggctcca tggccgcagt ccgacccgcg tctcgatgat ctcgcagccg ccgagccccg
    12601 gcgcgatc
//
LOCUS       DATAOZ010000407         3596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2533886, whole genome shotgun sequence.
ACCESSION   DATAOZ010000407 DATAOZ010000000
VERSION     DATAOZ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2533886"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VHR64_10390"
     CDS             <1..1158
                     /locus_tag="VHR64_10390"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3303279.1"
                     /translation="RWHAAPGKPTLLIYGHYDVQPVDPLDLWTSDPFEPTVRDGKIYA
                     RGVSDMKANLVTVVQAVEALALANCAPPVNLTFFFEGEEEIASPHAADVLTQYRDRFA
                     ADLVLSCDGGQAGPDQAKMTVAFKGVTGLQINLSTGSTDLHSGGYGAAVPNAAQAIAK
                     LAASFHDENGRVLVEGFYDDVIDLTDEDRVEFKTAETPDDELMAEAGVRALWGETGYT
                     AQERRGGRPTIDVNGIWSGFQGAGVKTVTPCLAHLKVTSRLVANQDNCRIAELIKAHA
                     LRHAPAGTEITFSDQEEGSKPYMAPRGFIGERVARQVLEAHYGSPPQIARAGGSVPIL
                     ATFKDILGVDTVTIGFGLPGSQAHAPNEWYLESQFDRARSVYAAFLEALGE"
     gene            1155..2582
                     /locus_tag="VHR64_10395"
     CDS             1155..2582
                     /locus_tag="VHR64_10395"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX3303280.1"
                     /translation="MTTAQEYLDQRQDRNLEQVMELLRIPSVSTDPAHAGDVHSAAEW
                     IAERMRAIGVPEVRLVDTPRHPLVYGKWHAAPGAPTILVYGHYDVQPVDPLNEWVSPP
                     FEPTIRDDRIYARGAADMKANLVTLLQAVEALAGTSPIGAPPINLTFLWEGEEEIGSP
                     SAPAVVEAMKDELAADVIMSCDGGMYDGDTGALWISFKGLAAIELIIRTGSTDLHSGG
                     YGAVVPNAAQVLAQVAASMHDDHGRVAVPGFYDGVVELSDADRAEIAAMAPSDDALRR
                     ETSVKALWGEPGYTPEERRSARPTLDINGYWSGFQGAGGKTVTPCLGHIKITCRLVPD
                     QDPDRIAKLIAAHAQSVVPAGVTVEPRFSERNGRGYAISRDNPFLARLGEVLEAEYGT
                     PARVVRVGGSVPITAMFRQLLRRETITLGFLLPASNVHAPNEWFRISDLNRARSVYAS
                     FLSGWQGEDIGTGVPSPGVRKENGQ"
     gene            2661..3182
                     /locus_tag="VHR64_10400"
     CDS             2661..3182
                     /locus_tag="VHR64_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX3303281.1"
                     /translation="MAGLMTRIATLAAILSLTLGVLLVRPGTADAALWTPDANECAFL
                     SAINLYRKANGVPAVSLSRSLSMAADYHSAYMAKTDDVDHTLSGGASWSSNILNFGYP
                     AGYGMGENVLAGRSSAGGALNLWKTSPPHNANMLNASWKAIGIGRAVNSNGYYKYYWT
                     TTFGTVRHRTISC"
BASE COUNT          650 a         1181 c         1143 g          622 t
ORIGIN      
        1 cgctggcacg ccgcgcccgg caaaccgacg ctcctcattt acggccacta cgacgtccag
       61 cccgtcgatc cgcttgacct ctggacgtcc gatccgtttg agccgaccgt tcgggacggc
      121 aagatctatg cccgcggcgt ctcggacatg aaagcgaacc tggtcacggt cgtgcaagcg
      181 gtggaagcgc tagccttggc caattgtgcg ccgccggtga atctcacctt cttcttcgag
      241 ggcgaagagg agatcgccag cccgcacgcg gccgatgtcc tcacccagta cagggaccgc
      301 ttcgccgccg acctggtgct ctcgtgcgac ggcggtcagg ccgggccaga tcaggccaag
      361 atgaccgttg ccttcaaggg cgtgaccggg cttcagatca acctctcgac gggttcgacg
      421 gatctccact caggtggata cggcgcggcc gtcccgaatg ctgcccaggc gatcgcgaag
      481 ctggccgcat ccttccacga cgagaacggc cgcgtcctgg tcgagggctt ctatgacgac
      541 gtcatagatc tgactgatga ggaccgcgtc gagttcaaga cggccgagac ccctgatgac
      601 gagttgatgg ccgaagccgg ggttcgcgcg ctctggggcg aaaccggata cacagcccag
      661 gagcgccggg gcgggcggcc gacgatcgac gtcaacggca tctggagcgg cttccaggga
      721 gcaggcgtca agacggtcac gccctgcctc gcgcacctca aggtcacgtc ccggctcgtt
      781 gcgaaccagg acaactgccg aatcgctgag ctgatcaagg cgcacgcgct ccgccatgcg
      841 ccggcaggca ccgagattac cttctcggat caggaggagg gctcgaagcc gtacatggcg
      901 ccgcgtgggt tcatcggtga gcgcgtcgcc cggcaggtcc tggaagcgca ttacggctcg
      961 ccgccgcaga tcgcgcgcgc cggcggctcc gtcccaatcc tcgccacctt caaggacatc
     1021 ctcggcgtcg acacggtcac gatcggcttc ggattgcccg gctcccaggc gcacgcgccg
     1081 aacgagtggt acctggagag ccagtttgat cgcgcccggt ccgtctacgc ggcattcctc
     1141 gaggcactcg gggaatgacc accgcgcagg agtacctgga ccagcggcag gatcggaacc
     1201 tcgagcaggt catggagctg ctccggatcc cgagcgtcag cactgaccca gcgcacgccg
     1261 gcgatgtcca ctccgccgcc gagtggatag ccgagcggat gcgcgcgatc ggtgtccctg
     1321 aggtccgtct ggtcgacacg ccgcggcatc cccttgtcta cggcaaatgg cacgccgcac
     1381 ctggtgcacc gacgatcctg gtctacggcc actatgacgt gcagccggtc gacccgctca
     1441 acgagtgggt ctcgccgccg ttcgagccga ccatccgcga cgaccgcatc tacgcgcgtg
     1501 gtgcggccga catgaaggca aacctggtca cgctgctcca ggccgtcgag gctctggccg
     1561 ggaccagccc aatcggcgcg ccgccgatca acctgacctt cctctgggag ggtgaggagg
     1621 agatcggcag cccgagcgcc ccggcggtag tcgaggcgat gaaggatgag ctggccgctg
     1681 atgtgatcat gtcctgcgat ggcggcatgt atgacggcga caccggcgcg ctctggatct
     1741 cgttcaaggg tctggccgct atcgagctca tcatccggac cggctcaact gacctgcact
     1801 ccggcggata cggcgcggtc gttccgaacg ccgcgcaagt cctggcccag gtcgccgcca
     1861 gcatgcacga cgaccacggc cgcgttgccg tgcccggctt ctacgacggc gtggtcgagt
     1921 tgagtgacgc ggatcgcgcc gagatcgcgg ccatggcgcc gtcggacgac gccctccgtc
     1981 gcgagacatc ggtcaaggca ctctggggcg agcccgggta cacgccggag gagcgtcgat
     2041 cggcacggcc gacgctcgat atcaacgggt actggagcgg cttccagggt gcaggcggca
     2101 agaccgtcac gccctgcctt ggacacatca agatcacgtg ccgcctggtc ccggatcagg
     2161 atcctgaccg catcgcgaag ctgatcgcgg cccacgccca gtcggtcgtc ccggccggcg
     2221 tcacggtcga gccgcggttc tccgagcgaa atggtcgagg ctatgcgatt tcgcgggaca
     2281 atcccttcct ggcccgcctc ggggaggtac tcgaagcgga atacggcact cctgcacgag
     2341 ttgtgcgtgt cggaggttct gtgcctatta ccgctatgtt taggcaactt ttacgtcgag
     2401 aaaccattac gctcggtttc ttgttgccgg catcgaacgt acacgcccct aatgagtggt
     2461 ttagaatcag cgatctcaac cgagcgcgct cggtgtacgc ctcgttcctg tctggctggc
     2521 agggtgagga catcggtacc ggcgtgccta gcccgggggt aagaaaggag aacggacaat
     2581 aggtcaattg tgcaagcgca ccccgtggct gaaggtggtt gagaaacacc tgcggctgat
     2641 cacgggggac gttttcttca atggctggac tcatgacgcg gatcgcgacg ctggctgcga
     2701 tcctctcact cacacttggc gtgctgctgg tacgcccggg caccgctgac gcggcccttt
     2761 ggacgcctga cgccaacgag tgcgcatttc tgagcgcaat caacctttac cgaaaagcaa
     2821 acggggtgcc tgctgtatcg ctgtcgcgat cgttgagcat ggcggccgac tatcactctg
     2881 cctacatggc gaagaccgat gatgtcgacc acacgctttc gggcggggca tcctggagct
     2941 ccaatatcct gaacttcggc tatccggccg gatatggaat gggcgaaaac gtcctggcgg
     3001 gacgatcatc cgccggcgga gcgctgaatc tctggaagac atcgcctccg cataacgcca
     3061 acatgctcaa cgcgagctgg aaggcaatcg gcattggccg agcggtcaac tccaacgggt
     3121 attacaagta ctactggacg acgaccttcg gcacggttcg ccaccggacc ataagctgtt
     3181 aaatctactt tccacccgca ggtgtttcga agaccctggc gcgctcgcaa aagcgccggg
     3241 gtcttccttt tcttttggcc gagtcgacgt accgacccca gaatcgtagc gcggctcgcc
     3301 tctaggtccg ttctggctcc gggagcagaa tcgcgttgcg ccgggccgct ccgtcaaccc
     3361 tgcccccatc gtgagcacaa gcccggccac ggggggccgg cgctacggca tcgcgagcta
     3421 tcaccagacg atcgacgtag cgtggggtta ccacgccccg cttgatgcaa cctcacacgc
     3481 catagatgga gccgaacgat tgtcgggatc ggttccgcga atggaccgac cggcatcggg
     3541 cccgggcggg gcgtgatccc ctctccggct ctccccattg gggagaaagc atcccc
//
LOCUS       DATAOZ010000408         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3850421, whole genome shotgun sequence.
ACCESSION   DATAOZ010000408 DATAOZ010000000
VERSION     DATAOZ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3850421"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VHR64_10405"
     CDS             complement(<1..1080)
                     /locus_tag="VHR64_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEX3303282.1"
                     /translation="MSELAKLISDLVRINSVNPSLDPTGPGEGEIAVFVADWMKRAGL
                     AVAMQESAPGRPNVIGIRRGTGGGRSLMLNAHMDTVSFGGMADPLSGRVDGNRLYGRG
                     SYDMKASLAAAMLAVADLGDEPLRGDVIVTAVTDEEYASIGTQAIVREYTADACVITE
                     PSHLEMCIAHKGFVWAEIETHGTAAHGSLPDVGVDAITLMGPILTGIGELDRRLRAGA
                     GHPLLKTGSLHASLIEGGTELSTYPDRCRVQIERRTVPGETDAMVEQQLRELIGDAAD
                     LAMGISRQPFEIGPEAELVQAMQAVVTKRLGRPAEIVGWGGWMDSALTSAAGIPSLVF
                     GPTGHGAHADDEWIDLDSVQTCREIY"
     gene            complement(1077..1688)
                     /locus_tag="VHR64_10410"
     CDS             complement(1077..1688)
                     /locus_tag="VHR64_10410"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX3303283.1"
                     /translation="MRLTNVGILIFDGVEVLDFCGPFEVFSLAIKPGMDTYPDNALYK
                     VHTVAETANSIRTVGGMEVVPTCTIDDHPEFEIIVVPGGFGTRAIYQERPRVTDWIAR
                     QAAADVLTTSVCTGARLLAKAGVLAGKRSTTHWASIGRLREQHPETTVLDDLRVVDEG
                     NVVTSAGVSAGIDMALHIVARMHGRDAAAATARQMEYRWEPVA"
     gene            complement(1685..2413)
                     /locus_tag="VHR64_10415"
     CDS             complement(1685..2413)
                     /locus_tag="VHR64_10415"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX3303284.1"
                     /translation="MNPDLSHARSNRSINGDPVEIELPRPRPERIVHEGRRVTVEPLD
                     AEKHAAELYPLLHGDAEKERVWDHLPYGPFPSFEAYRDWVADVASKGDPLFHAIRNTR
                     TGRVEGVASLMEIRPAPGVIEIGHINLSTALQNTIEATEGLYLLMTYAMDELQYRRFE
                     WKCNALNENSRRAAARLGFSFEGIFYQHSVPKGRNRDTAWFSTLDYEWPAIRQNFERW
                     LSPDNFDADGRQRTSLGEMNRSIP"
     gene            complement(2410..>3332)
                     /locus_tag="VHR64_10420"
     CDS             complement(2410..>3332)
                     /locus_tag="VHR64_10420"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303285.1"
                     /translation="VVFVVGAFNLLFDVSEGSFIPFVIPRDRLVEGNSKIEASYSTAQ
                     TAGPAIGGALVTILTAPYAVLVTAVGLIGSAGFLSRMTTPESQPHIDHERSVRQDIVE
                     GLRFVRHHTLLRPILLTMIGQSFFGFVFMAVYVLYMKRNLEISDFQVGLVFAAGGVGS
                     LLGTLATPALNNRFGIGPVMVAGNMFFGVTGLLIPLAVLIPEYAFPLVIVCEFLQYMG
                     LLPFFLNAISLFQLVSPDEMRGRIMSTRKFLTWGVQPFGSLLGGILGGLITLPWTLAV
                     GEIGLLAVAIWLCLHPIRSLRSVPVEREVG"
BASE COUNT          576 a         1193 c         1027 g          536 t
ORIGIN      
        1 gtatatctcg cggcaggtct ggactgagtc gagatcgatc cactcatcgt ccgcgtgcgc
       61 gccgtgacca gtcgggccga agacgaggct cgggattccg gccgcggacg tgagagcgga
      121 gtccatccag cctccccagc cgacgatttc cgccggccgc ccgagtcgct tcgtgacgac
      181 tgcttgcatc gcctggacca gctcggcctc cggcccgatc tcgaacggct ggcggctgat
      241 gcccatcgcc aggtcggccg cgtcgccgat cagctcgcgg agctgctgct cgaccatcgc
      301 gtccgtctcg ccgggcaccg tccgccgctc gatctggacc cggcagcgat ccgggtaggt
      361 cgagagctcg gtaccgcctt cgatcagtga ggcgtggagc gagccggtct tgagcagcgg
      421 atgccctgcc ccggcgcgca accgccggtc gagctcgccg atgccggtca agatcggccc
      481 catcagcgtg atcgcgtcca caccgacgtc cggcagcgag ccgtgcgcgg ccgtgccatg
      541 ggtctcgatc tcggcccaga cgaatccctt gtgggcgatg cacatctcga ggtgcgacgg
      601 ctcggtaatg acgcaggcgt cggccgtgta ctcgcgcacg atcgcctgcg tgccgatgct
      661 ggcgtactcc tcgtccgtca ccgccgtcac gatcacgtcg ccgcgcaacg gctcatcgcc
      721 caagtcggcg acggctagca tcgccgcggc caggctcgcc ttcatgtcgt acgagccgcg
      781 cccgtagagc cggttcccgt cgacccggcc cgagagggga tcggccatcc cgccgaagct
      841 gaccgtgtcc atgtgcgcgt tgagcatcag cgagcgcccg ccgccggtac cgcgccggat
      901 cccgatcacg ttcggtcgcc cgggcgcaga ctcctgcatc gcgactgcca gccccgcgcg
      961 cttcatccag tcggccacga agaccgcgat ttcgccctcg ccgggaccgg tcgggtcgag
     1021 actcggattg actgagttga tccggaccag gtcgctgatc agcttcgcca gctcactcac
     1081 gccaccggct cccatcgata ctccatctgc cgcgcggtcg cagccgccgc atcccgcccg
     1141 tgcatccggg ccacgatgtg cagcgccatg tcgatcccgg cgctgacccc cgccgaggtc
     1201 acaacattgc cctcgtcgac cacccgcaga tcgtccagca cggtcgtctc cgggtgctgc
     1261 tcgcgcaatc ggccgatact cgcccagtgc gtggtcgagc gcttgcccgc cagcactccc
     1321 gccttggcca gcagcctcgc gccggtgcag accgacgtcg tcaggacgtc cgccgcagcc
     1381 tgccgggcaa tccagtcggt cacccgcggc cgctcctggt agatcgcccg tgtcccgaaa
     1441 ccgcccggca ccacgatgat ctcgaactcc gggtgatcgt cgatcgtgca ggtcgggacc
     1501 acctccatcc cgccgacggt ccggatcgag ttggccgttt cggcgacggt atggaccttg
     1561 tagagtgcgt tgtcggggta ggtgtccatg ccaggcttga tcgccagcga gaagacctcg
     1621 aagggtccgc agaagtcgag cacttcaaca ccgtcgaaga tcaggatgcc gacattggtc
     1681 aacctcacgg aatgctcctg ttcatctcgc caagcgacgt acgctggcgt ccatccgcat
     1741 caaagttgtc gggcgagagc cagcgctcga agttctgccg gatcgccggc cactcgtagt
     1801 cgagcgtcga gaaccaggca gtgtcccggt tgcgaccctt ggggaccgag tgctggtaga
     1861 agatgccttc gaagctgaaa ccgagccgcg ccgccgcccg ccgcgagttc tcattgagcg
     1921 cgttgcactt ccactcgaac cgccggtact gcagctcgtc catcgcgtag gtcatcagca
     1981 ggtagagccc ctcggtcgcc tcgatcgtgt tctgcagtgc ggtgctgaga ttgatatggc
     2041 cgatctcgat cacgcccggg gccggccgga tctccatcag cgacgccacg ccctcgaccc
     2101 ggcccgtccg cgtgttgcga atcgcgtgga agagcggatc acccttgctg gccacgtccg
     2161 cgacccagtc ccggtacgcc tcgaagctcg ggaacggacc gtacggcagg tgatcccaga
     2221 cccgctcctt ctcggcgtcg ccgtggagca ggggatagag ctcagccgca tgcttctccg
     2281 cgtcgagcgg ctcgaccgta acccgccgcc cctcgtgcac gatccgctcc ggccgcgggc
     2341 gcggcaattc gatctcgacc gggtcgccat tgatcgaccg gttcgaccgc gcgtgactca
     2401 gatccgggtt catccgacct ccctttcgac cgggaccgac cgcaacgacc ggatcggatg
     2461 caggcagagc cagatcgcga ccgccagcaa gccgatttct cccactgcca gcgtccatgg
     2521 tagcgtgatc agcccgccca gaattccgcc cagcagcgag ccaaacggct gcacgcccca
     2581 ggtcaggaac ttgcgagtgg acataatcct gccccgcatc tcgtccggcg agacaagctg
     2641 gaacagcgag atcgcattca ggaagaacgg caacaagccc atgtactgca ggaactcgca
     2701 gacgatcacc agcggaaagg cgtactccgg gatcagcact gcgagcggga tcagcaaccc
     2761 ggtcacgccg aagaacatgt tccccgccac catcaccggc ccgattccga accggttgtt
     2821 cagcgccggt gtcgccagcg tcccgagcag ggacccgacc ccgcccgccg cgaagaccag
     2881 cccgacctgg aagtcgctga tctccaggtt ccgcttcatg tacagcacgt agaccgccat
     2941 gaagacgaag ccgaagaagc tctgcccgat catggtcagc aggatcgggc gcaggagggt
     3001 gtgatgtcgc acgaagcgca gcccctcgac gatgtcctgc cggactgagc gctcgtggtc
     3061 gatgtgcggc tgcgattcgg gcgtggtcat ccggctcaga aagcccgccg agccgatcag
     3121 gccgaccgcc gtgaccaaaa cggcataggg cgcagtcagg atcgtgacca acgcgccgcc
     3181 aatcgcggga cccgctgtct gcgcggtcga ataactggcc tcgatcttcg agttgccctc
     3241 gaccagccgg tcccgcggaa tcacgaacgg gataaacgag ccctcgctga catcgaacag
     3301 caggttgaac gcgccgacca cgaacacgac ag
//
LOCUS       DATAOZ010000409        13802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7004839, whole genome shotgun sequence.
ACCESSION   DATAOZ010000409 DATAOZ010000000
VERSION     DATAOZ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13802
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7004839"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VHR64_10425"
     CDS             complement(<1..304)
                     /locus_tag="VHR64_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX3303286.1"
                     /translation="MTTERIALVRETLASIGTDALILTNPANRRWATGFTSAAGIAFS
                     PDVAVITPSTVELLVAAIHADWARGEAVVATNVMAHKGQFAPSLIAFLNERGYGSVA"
     gene            complement(301..747)
                     /gene="aroQ"
                     /locus_tag="VHR64_10430"
     CDS             complement(301..747)
                     /gene="aroQ"
                     /locus_tag="VHR64_10430"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX3303287.1"
                     /translation="MAKRILVLNGPNLNMLGTRQPEIYGRTTLGEIVSAMRDLAESLA
                     PGVDLSFFQSNHEGALIDAVQQSTGEIDAIIINPGGLTHTSVALRDALVGADRPVVEV
                     HMSNIHARESFRHHSYIAPIAVGQIAGFGAAGYLLALRSVIENVLA"
     gene            complement(774..1769)
                     /locus_tag="VHR64_10435"
     CDS             complement(774..1769)
                     /locus_tag="VHR64_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303288.1"
                     /translation="MKLVRIPPVFWVALLALIAFQLAGPSATYGTTVPVTPTPDGTDN
                     KGSDSELSPELPPPDFPSANVEGYTFDLRYALSAELNKVPREASVYELLRDEPTVDSV
                     QKIADNLAIDGKVEDRGDGSFTATGDGGDLFVTIDMINYFSSAQVPEGDLPSDEKAIA
                     AGRDWLRHASLLPPDIGTGSVVSRMPDSNRLIVNFVPAEPVPILSGYPSITLTMAVDG
                     SVIEAQIRWANIVRSDVYQLMSPDQAWQLVTTGQAYLAPDLRGAGIDPGTEVKGRVTF
                     TAISIAYATAGPPGGRQYLQPVYVFEGRIRPEGDKKTYPITAYVSALSNSGAPVG"
     gene            complement(1773..3812)
                     /gene="uvrB"
                     /locus_tag="VHR64_10440"
     CDS             complement(1773..3812)
                     /gene="uvrB"
                     /locus_tag="VHR64_10440"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX3303289.1"
                     /translation="MPDFQIVADIKPTGDQPKAIAGLVEGLRADERHQTLLGVTGSGK
                     TFTMANIIQEVNRPTLVLAHNKTLAAQLYAEFKEFFPRNAVEYFVSYYDYYQPEAYVP
                     RSDTYIEKTADINDEIDKLRHAATRSLLTRRDTLIVASVSCIYGLGSPEEYQNTVVQI
                     RRGANLRRDKVMRHLIDIQYDRNDMSLIRGTFRARGDTLEIYPAYEEVAVRVEFFGDE
                     VERIVEVDPLTGELLVERLEIDIYPAKHFVTNSEKLALAIRDIREELDQQVERLESAG
                     KILEAARLKQRTLYDVEMLTETGYCAGVENYSMHLSRRRPGEQPSTLMDYFPDDFLCF
                     IDESHISIPQVRGMYAGDRSRKEVLIDFGFRLPSALDNRPLRFDEFEKMVKQVVYVSA
                     TPGPYEMEHSERVVEQVIRPTGLVDPAISVRPTKGQIDDLLGEINTRVAKGERVLVTT
                     LTKRMAEDLADYLKEMGVRTHYLHSEIDTFQRVEILRDLRLGIYDVVVGINLLREGLD
                     LPEVSLVAILDADKEGYLRSEGSLIQTVGRAARHVDGQVIMYADNVTRSMRAAIDETY
                     RRRALQTEYNEAHGIQPRGIVKEIKDLTDRVKAAAEESAGYDTGAASKAGVIAELPKD
                     EVLRIVKDLESQMKIAARDLEFEKAALLRDQIVELRRMMEVDPIAASGRPMSGAM"
     gene            complement(3890..4348)
                     /locus_tag="VHR64_10445"
     CDS             complement(3890..4348)
                     /locus_tag="VHR64_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303290.1"
                     /translation="MPQLRSDVVEVFVVRWRNGQIETLLGKRRAQQPIGSIWQPFSAR
                     LDLGEATIEGAKRAVVRGCGLQVTELFAIDRTHQFFDHQRDMIVIAPVLAAMVHATAP
                     RAGADFESVEWQDAPTAQNNLFIAGHRDSHRRVVELLGPGGTALDLYRLL"
     gene            5071..6624
                     /locus_tag="VHR64_10450"
     CDS             5071..6624
                     /locus_tag="VHR64_10450"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX3303291.1"
                     /translation="MTHEVRCRAPRLIPIAAFVTGFASIATEISASRLIAPYFGSSTF
                     IWANLIGVTLLFLSLGYWLGGRLIDTRPDARLLYGLIAVAAVCLALVIPISRPILRAS
                     LDAFDDRNAGAFLGSLAATLLLFAPSLLALGMVAPAAIRLRMSSVEDAGKAAGSLYAI
                     STAGSILGSFVPVIILLPEIGTRATFATIAAVMIIMSIVGFAAEGKPAIAIAIAGIFA
                     LAIGALILGSHGNLKPPYRGQLIAELESDNNYIQVLQDGDETLLALNDGHAVHSIYNP
                     NRLETGGPWDYFALGPVLTGDTAPNRALIVGLAGGTAARQLHAAYPSITVDGIEIDRT
                     IVDAGRKYFGLTDGVANVRIADGRYALETSDTKYDLVCLDAYRQPYVPFHLATVEYFQ
                     LVSQHLNPGGVVVVNAGRTDTDFRLVDALSATISKAFPTVLVVDVDRYDNSMIYATND
                     PVTPDQFAQRLRETSGIPLVQTVAGWALDHGNIRTASTSGTVFTDDKAPVEWIIDQII
                     FDEAVREEK"
     gene            6621..7451
                     /gene="pgeF"
                     /locus_tag="VHR64_10455"
     CDS             6621..7451
                     /gene="pgeF"
                     /locus_tag="VHR64_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX3303292.1"
                     /translation="MTVPTMAPMPAMELAQHVERSRLISAIPWVRHGVTRRVPGLGLA
                     DGNVGYTAPRDEADAWEMRQRWARAMGIDPSDLVRVRQVHGKAVRIATEEDTERGTHP
                     DADEAPIADSMITADPNVALMTLHADCLAMLLVDPIHRAVGAVHAGWRSTVQDIAGET
                     VQAMQASYGSRPEELIAFVGPSIGVDRYEVGDDVIDAWRSFAGSSHSAIRRVNGSWRF
                     DLKAANAAQLIARGVVPANIEISPVCTASQTDRWFSHRAQGSLTGRFAAVIAISKAGS
                     "
     gene            7448..8140
                     /locus_tag="VHR64_10460"
     CDS             7448..8140
                     /locus_tag="VHR64_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX3303293.1"
                     /translation="MTQITLASLEGRIGAVRESVEFAAERVGRQLSDVTIVAVSKTVD
                     RETMQAAYDLGLRHFGENRVQDARQKLAEPMPADCTLHLIGQLQTNKAGHAVDLFNVI
                     ESVDRQSLIVELEKQSAKRGITLPVLLQVNVAGEDQKAGCSPDDVDALVNQIVGSDHL
                     ALRGLMTIAPLVDDSEEVRPVFRRLREIRSRVQDDSGLKLPLLSMGMTNDFPVAIEEG
                     ATHVRIGRAIFG"
     gene            8152..8820
                     /locus_tag="VHR64_10465"
     CDS             8152..8820
                     /locus_tag="VHR64_10465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX3303294.1"
                     /translation="MDAELWTTVDAWFTERLIESDVGLEHALAEIDRAGLRQISVSHN
                     QGKLLYLLTRMTGARRILEVGTLGGYSAIWFARGLPEDGRLITLEIDPKTAEVARANL
                     TNAGVDHLVEVRIGAALESFQAIADEEHDPFDIVFIDADKENNPAYFEWALKLTHPGS
                     LILIDNVARRGRVVDLSESEPDIMGVRRVVDMIANEPRVTATAIQTVGEKGYDGFLIA
                     LVTA"
     gene            complement(8821..11679)
                     /locus_tag="VHR64_10470"
     CDS             complement(8821..11679)
                     /locus_tag="VHR64_10470"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX3303295.1"
                     /translation="MFRQFRRVAAKPTGTPRRPRTPVIPKITISPANKRRIIAALIIV
                     GVIVAVFVSARFWMNWWWFNSMGYRSVLLRRAALQVAAFLVVALIGFGIVGGSASLAF
                     RRTRQEVSRSTFVRFANRLLFWIAVAVSVVTGVGLAWWTANRWETFALWWNGRSMGIK
                     DPIYHRDASFYLFALPALDLIWKVAAIATLIAFVLTAFIYLIRLGLNLRNVGKLPATA
                     FRHLLALIGVLLLLGSIWFYLDNFHLVYSTRGAAYGVSYTDDHAIRIANWVAIIALIV
                     AGAACIASRRVPKRREAFLAALVVIFLSIGVQGLLPLVVKQAIVEPDELNKERPYIAN
                     NIAMTSYAYGLGDVQEGELAGTGTITSQDIAENPELVSNVRLWDYRVARTTYQELTSY
                     APYYQYLDVDIDRYVVNGVEQPVLVSARELNQDGLPSNALTWTNRRLVYTHGYGAVVS
                     PVDEATQSGLPVFTVSQIPPNGSGPFTITHPEIYYGEADLGWVIVGTKVQEFSALPEV
                     DQTTSLTQDEIVGGIRLDDSVTKLISALALKDRNVFISGNIQDSSTLLLNRNIEERIK
                     LIAPFLTLDPDPYLVIADGQLVWVVDAFTTSSKFPMSTPQDGINYIRASVKITVDART
                     GATTFYRTAELDPIADAYERMFPSLFEPISTAPATVAAHFRYPEPIYDVQSRIYGAIH
                     VDDPDQYYNGEDRWTVPGSLASEDLLENKSERDEMPAYYITVPLPGETNAKFTLVRPF
                     VPGGNSVRKNMTAWMAGRSGPDGSLTLSVYRFPRQTNVFGPAQVEARIDQDPAISQQI
                     SLWNQSGSQVLQGALLIIPVKQTVLYVQPLYLRSTSGSSIPELRRVIVATEQNVFMAD
                     TLDKALAGALTGQESSVTPGTEGGGGEPVASGDMQALVQQANDAYQRSQDALARGDWA
                     AYGQAQAELASILHQMETLTGGQATPTANQPIVATPAP"
     gene            11742..12587
                     /locus_tag="VHR64_10475"
     CDS             11742..12587
                     /locus_tag="VHR64_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3303296.1"
                     /translation="MNSTEPILQSNAPIRIRSLASGSSGNAYLVESGDDLVLIDCGIG
                     IRQIQAALHAWGKTLRDLSAIVISHEHSDHVRSLASFLRQRTPIYATGGTSDALGLSS
                     SCLSTIRYTESFDIGCMAITPIATSHDAAEPCGLLIEASERTIGLITDLGTVTDEIGT
                     FASRCELLILESNHDRDMLRSGPYPAHLKKRVAGNFGHLSNDQAGGFLAEIATATSGP
                     SEIWLAHLSATNNTPTVARAAIQDRFRKAGVSPAITVLPRGAPGPIWDGAIVRAKQLA
                     LISDF"
     gene            12696..12989
                     /locus_tag="VHR64_10480"
     CDS             12696..12989
                     /locus_tag="VHR64_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303297.1"
                     /translation="MFTFMRGGVMEGSISGSLVIGQVLIGVQGLMGLIMFADGFRPAD
                     SVHILYGIAAFVTMPFVWSYARERHPRQGLFFYSLAALFIAGLAIRGITTGGG"
     gene            13133..13675
                     /locus_tag="VHR64_10485"
     CDS             13133..13675
                     /locus_tag="VHR64_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3303298.1"
                     /translation="MDTQARDYQEVIDQALGLVYSHHNRLITRLAPSTFVPLDIEDLR
                     EGPLGRDLLRLANLARGQTREEKELVLRAIEDVVQLLFWPAAADEYTVPRSFWDTDLG
                     RMMALAKFRAYEPHELVSIGVAAETLQVTRPTIYRWMDDRRLNYVKDEISGRTFIVRR
                     DLDNWNTVGSDLDVEASSVF"
BASE COUNT         2572 a         4404 c         4277 g         2549 t
ORIGIN      
        1 ttgccaccga tccatatccg cgctcgttca gaaacgcaat gaggctgggc gcgaattgcc
       61 ccttgtgcgc catgacattg gtcgcgacca ccgcttcgcc ccgtgcccaa tcggcgtgga
      121 tcgcggcgac cagcagttcc acagtgctcg gggtgatcac cgcaacgtcg ggggaaaacg
      181 cgattcccgc cgccgatgtg aatcccgtgg cccagcggcg attcgccggg ttggtcagga
      241 tcagtgcatc cgtgccgatc gatgcgaggg tctcgcgaac aagggcgata cgttcagtcg
      301 tcatgccagc acgttctcga tcacggagcg aagggcaagc aaatacccgg ccgcgccaaa
      361 ccccgcgatt tgaccaacag caatcggcgc gatgtaggaa tggtgccgaa acgattcccg
      421 ggcgtggatg ttggacatgt gaacttcgac gactggccga tccgctccga ccagtgcatc
      481 acgcaaggcg acgctggtgt gtgtcaatcc gccgggattg atgatgatcg cgtcgatttc
      541 gccggtcgac tgctggacag cgtcgatcag cgcaccttcg tgattcgact ggaagaagga
      601 aaggtcgaca ccgggcgcga gcgattcggc caggtctcgc atcgccgaca cgatctctcc
      661 gagcgtcgtg cggccgtaga tctcgggctg ccgtgtgccc agcatgttca ggttcggacc
      721 gttgagaacc aggatccgct ttgccattca ccccattccc ggtagagcag tgactaaccg
      781 accggagctc cggagtttga gagcgccgag acgtacgccg tgatcggata ggtcttcttg
      841 tctccctccg gtcgaattcg gccttcgaag acgtagaccg gctgaagata ctggcgcccg
      901 cctggaggac cagccgtcgc gtacgcaatc gaaatcgcgg tgaatgttac ccggcccttg
      961 acctccgttc cgggatcaat cccggccccg cgcaaatcag gagcgagata ggcctggcca
     1021 gtcgtgacca gctgccacgc ctgatccggc gacatcagct gatagacgtc ggaccggacg
     1081 atgttggccc agcgaatctg cgcttcgatg accgatccat cgaccgccat agtgagcgtg
     1141 atgctcgggt atccggacag aatcggcacc ggctccgccg gcacgaaatt gacgatgagc
     1201 cggttcgagt ccggcatgcg cgagacgacg gaacccgtgc cgatatccgg cggcaggagc
     1261 gaagcgtgcc gcaaccagtc cctgccggcc gcgattgcct tctcgtccga aggcagatca
     1321 ccttccggaa cctgcgccga cgagaagtaa ttgatcatgt cgatcgtgac gaacaggtcg
     1381 ccgccgtcgc cggtcgcggt gaaggagcca tcgccgcgat cctcgacctt gccgtcgatg
     1441 gcgaggttgt cggcgatctt ctgcacgctg tcgaccgtcg gctcgtcgcg cagcagctca
     1501 tagaccgagg cttcgcgcgg gactttgttg agctcggcac tgagtgcata ccgaagatcg
     1561 aaggtgtatc cctcgacgtt ggccgacggg aaatcgggcg ggggaagctc aggcgagagc
     1621 tcgctatcgc tgcccttatt gtcggttcca tctggggtcg gggtgaccgg gaccgtcgtg
     1681 ccgtacgtgg cagagggacc ggcgagctga aaggcgatga gcgcgaggag cgctacccag
     1741 aacaccggcg gaatgcgaac caacttcacg gactacattg ctcctgacat gggtcggccc
     1801 gatgccgcga tcgggtcgac ctccatcatc cggcgcagct ccacgatctg atcgcggagc
     1861 aatgcggcct tttcaaactc gagatcgcgc gcggcgatct tcatctggct ctcgagatcc
     1921 ttgacgatcc ggagcacctc atccttcggc aactcggcaa ttacgcccgc cttggatgcg
     1981 gcgccggtgt catacccagc cgactcctcg gccgcagcct tgacgcgatc ggtgaggtcc
     2041 ttgatctcct tgacgatgcc acgcggctgg atgccgtgcg cctcgttgta ctcggtctgg
     2101 agcgcgcgcc ggcgatacgt ctcgtcgata gcggcacgca tcgatcgggt cacattgtcg
     2161 gcgtacatga tgacctgccc gtcgacatgg cgagcggcgc gaccgacggt ctggatcagc
     2221 gagccctcgg agcggaggta cccctccttg tcggcgtcga ggatcgcaac cagcgagact
     2281 tcgggcagat ccagaccctc gcgaagcagg ttgataccga ccacgacgtc gtagatgccg
     2341 aggcgcaagt cgcgcaggat ctcgacgcgc tggaacgtgt cgatctcact gtgcaggtag
     2401 tgcgtgcgga cacccatctc cttcaggtag tcggcaaggt cctcggccat ccgcttggtc
     2461 agcgtcgtga cgagaacccg ctcgcccttc gccacccgcg tgttgatctc gccgaggaga
     2521 tcgtcgattt ggcccttggt cgggcgcacg gagatggccg gatcgaccag accggtcggc
     2581 cggatgacct gctcgaccac gcgctcgctg tgctccatct cgtacggacc gggcgtcgcg
     2641 ctaacgtaga cgacctgctt gaccatcttc tcgaactcgt cgaaccggag cggccggttg
     2701 tcgagcgctg agggaaggcg gaagccgaag tcgatcagca cctccttgcg ggagcgatcg
     2761 ccggcataca tcccgcgcac ttgcgggatc gagatatgcg actcgtcgat gaagcagagg
     2821 aagtcgtccg ggaagtagtc catcagcgtc gacggctgct cgcctggccg acgccgggag
     2881 aggtgcatgg agtagttctc gactccggcg cagtagccgg tctcggtcag catctcgacg
     2941 tcgtagagcg tccgctgctt gagccgcgct gcttccagga tcttgccggc cgattccagc
     3001 cgctcgacct gctgatcgag ctcctcgcgg atatcccgga tcgccagcgc gagcttttcg
     3061 gagttggtca cgaagtgctt ggccgggtag atgtcgatct cgagccgctc gaccagcagc
     3121 tcgccggtga ggggatcgac ctcgacgatc cgctcgacct cgtcaccgaa gaactcgacc
     3181 cggacggcga cctcttcgta ggccgggtag atctcgagcg tgtctccgcg agcacggaag
     3241 gtgccgcgga tgagcgacat gtcattccgg tcgtactgga tgtcgatcag gtgccgcatg
     3301 accttgtcgc ggcggaggtt cgccccgcgc cgaatctgga cgacggtgtt ctggtactcc
     3361 tcgggcgagc cgagaccgta gatgcacgag acagacgcga caatcagcgt gtcgcgacgg
     3421 gtcaggagcg atcgggtggc ggcatgacgg agcttgtcga tctcgtcgtt gatatccgct
     3481 gtcttctcga tataggtatc cgagcgcggg acgtacgcct ccggctggta gtagtcgtaa
     3541 taggagacga agtactcgac cgcgttgcgc gggaagaact ccttgaactc ggcatagagc
     3601 tgcgcggcca gcgtcttgtt atgggcgagc acgagcgtcg gccggttgac ctcctggatg
     3661 atgttggcca tcgtgaaggt cttgcccgag ccggttacgc cgagcagcgt ctgatgccgt
     3721 tcgtcggcgc gcagcccctc aacgaggccc gcgatcgcct tgggctggtc gccggtcggt
     3781 ttgatgtcgg cgacgatctg aaaatcgggc atttgcctgt cctgactcac ggcaggtcga
     3841 gccgtgttgc aagcgacgag tatagccggg acggatatct ggcccgggat tagagcagtc
     3901 gatagaggtc gagcgcggtc ccgccggggc cgagcaactc gacgacgcgg cggtgcgaat
     3961 cgcgatgtcc ggcgatgaag aggttgttct gggcggtcgg ggcatcctgc cattcgaccg
     4021 actcgaaatc tgccccggct ctcggcgcgg tcgcgtggac catggccgcg aggaccggag
     4081 cgatcacgat catgtcgcgc tggtgatcga agaactggtg cgtgcgatcg atcgcgaaga
     4141 gctccgtaac ctggagtcca catccgcgca cgaccgcgcg cttggctccc tcgatcgtcg
     4201 cttcgccgag atcgagccgc gcgctgaatg gctgccagat cgatccgatc ggctgctgtg
     4261 cgcgtcgctt gccgagcagt gtctcgatct gaccattgcg ccaccggacc acgaagacct
     4321 caaccacatc ggatctcagt tgaggcatcg agttcagctc cttcagcgcg agcggctcac
     4381 ctattatacc agaacgggcg tgctaaccgg ttctgggcac tgggggaaat gatactgtcc
     4441 gatgaggacg aaactcgccc tgaaacaacc attcgcaagc tccatttcca cgtagtggaa
     4501 ataagaggag cgactgccag cccatctcga cccgcaactt tacatatcca ctttatgagt
     4561 ctcggcaacg ggggtggggg aaggagacgg gcaagctcga ccgcagcact gcgcgagaat
     4621 caactggtgc cagcgagcta gctcttgcac accaggtcat ctgcgctggc cagggatatc
     4681 cagcctggct tctgcgtcgg tcaggttctg cctggttaca atcgaaaccg gaaaaccgtc
     4741 catcaccaat tcttcgagcg atggcgtaca tccaacggtc gtgccgggcg cctcgccagg
     4801 catttgggcg gcatcgaacc gggcgagccg cccggcactt cgcagtcaaa ttcaaccccg
     4861 ggactcgagg gggaagccgc ggatcataaa ggctcctgga tcgccaaaat tcgtaatgcg
     4921 gataacatag attgcccgca atagtgtcag agaggctcca ggcacgacat aagtggcggg
     4981 ggagtcgaca taagggatcg taatccacgt cggtatactt ccccggagca gttcgtttag
     5041 cgtgtggatc gatcgaggcg cgttcttcag atgacacatg aggtcaggtg ccgcgcgccc
     5101 cgtttgatcc cgatcgccgc cttcgtgact ggcttcgcca gcatcgcgac tgagatcagc
     5161 gccagccgtt tgatcgcacc atacttcggc agctccacct tcatctgggc caacctgatc
     5221 ggcgtcacgc tgctcttcct gtcgcttggc tactggctcg gcggccgctt gatcgacact
     5281 cggcccgacg cccgcttgct ctacgggttg atcgcagtgg ccgccgtctg cctggctctg
     5341 gtcattccga tcagccgacc gattctgcgc gcctcacttg acgctttcga cgaccggaat
     5401 gccggcgcct tcctcggttc gctggccgcg acgctactgc tcttcgctcc gtccttgctg
     5461 gcgctgggaa tggtcgcgcc ggccgcgatc cgtctgcgca tgagctcggt cgaggatgcc
     5521 ggcaaggccg ccggttctct ctacgcgatc tccacggccg gctcaatcct gggcagcttt
     5581 gttccggtca tcattctcct tccagagata ggtacccgcg ccacgttcgc cacgatcgcg
     5641 gccgtcatga tcattatgtc catcgtcggc ttcgcggctg agggcaagcc tgccatcgcg
     5701 atcgccatcg cggggatctt cgcgcttgcg attggcgccc tgattctcgg gtcccacggc
     5761 aatctcaagc cgccctaccg cggccagctc atcgcggagc ttgagtccga caacaactac
     5821 atccaggtcc tgcaagacgg cgacgagacg ctactcgccc tcaatgacgg ccacgcggtg
     5881 cattcgatct acaacccgaa tcgtctcgag actggtggcc cctgggacta cttcgccctt
     5941 gggccggtcc tgactggcga caccgcgccg aaccgggcgc tgatcgtcgg gctcgccggc
     6001 ggaaccgccg cccgccagct ccacgcggca tacccgagca tcacggtcga cggcatcgag
     6061 atcgaccgca cgattgtcga tgccgggcgg aagtacttcg gtctcacgga cggcgtggcc
     6121 aacgtcagga tcgccgacgg ccgctacgcc ctcgagacct ccgatacgaa atacgacctg
     6181 gtctgcctcg acgcgtaccg gcagccctac gtgccgtttc acctggcgac ggtcgagtac
     6241 ttccagctcg tctcgcagca cctcaacccg ggcggagtgg tcgtcgtcaa tgccggtcgt
     6301 accgacaccg acttccgcct cgtcgacgcg ctgtcagcca cgatctcgaa ggcgttcccg
     6361 acggtcctcg tggtcgacgt cgaccgttat gacaacagca tgatctacgc cacgaacgat
     6421 cccgtcactc ctgaccagtt cgcccagcgc ctgcgcgaga cgtccgggat cccgctggtg
     6481 cagacagttg ccggttgggc gctcgatcat ggcaacattc gtacagcatc cacgagcggc
     6541 acggtcttca ccgatgacaa ggcgccggtc gagtggatca tcgaccagat catcttcgac
     6601 gaggcggttc gggaggaaaa gtgaccgtac ccacgatggc gcccatgccg gcaatggaac
     6661 tcgcccagca tgtcgagcgc tcgcgtttga tttccgcgat tccctgggtg cggcatggcg
     6721 taacccgacg cgttcccggt ctcggcttgg ccgacggcaa cgtcggatac accgcgcctc
     6781 gcgacgaggc agatgcctgg gaaatgcgcc aacgctgggc ccgggcgatg ggtatcgacc
     6841 cgtccgatct cgttcgcgtt cgccaggtcc acggcaaggc ggtccgcatc gcaaccgaag
     6901 aagataccga gcgcggaacg cacccggacg ccgacgaggc cccaatcgcc gattcgatga
     6961 tcacggccga tccgaacgtc gccctgatga cactccacgc ggattgcctg gccatgctgc
     7021 tggtcgatcc gatccatcga gcggttggcg cagtgcatgc cggctggcga tcgaccgtcc
     7081 aggacattgc cggcgagacc gtgcaggcca tgcaagcgtc ctacggcagc cgacctgagg
     7141 agttgatcgc gttcgtcggc ccatcgatcg gtgtggatcg atacgaagtc ggcgacgacg
     7201 ttatcgacgc ctggcggtca tttgccgggt catcgcacag cgccattcgg cgagtcaacg
     7261 ggagctggcg attcgacctc aaggcagcca acgcggcgca attgatcgcc cgcggcgtcg
     7321 taccagcaaa catcgagatc tcaccggtat gcaccgcgtc ccagactgat cgttggttct
     7381 cgcaccgagc ccagggatca ctcaccggac gcttcgcggc tgtcatcgcc atctcaaagg
     7441 cgggctcatg acgcagatca cgctggcatc gctggaaggc cgcatcggcg ccgttcgcga
     7501 atcggtcgag tttgcggccg agcgagttgg ccgccagctc tcggacgtca cgatcgtcgc
     7561 ggtctcgaaa actgtcgatc gcgagacgat gcaggccgct tatgacctcg ggctgcgcca
     7621 tttcggtgaa aatcgggttc aggacgcgcg ccagaaactg gcggagccaa tgcctgcgga
     7681 ctgcacgctg catctcatcg gtcagctcca gaccaacaag gccggccatg cggtggatct
     7741 gttcaacgtg atcgaatcag tggatcgtca gtcgctgatc gtcgagctcg agaagcaatc
     7801 cgcgaaacga ggcatcacgt tgccggtctt gttgcaggtg aacgtcgccg gagaagatca
     7861 gaaggccggc tgctctccag acgacgtcga cgccctcgtc aaccagatcg tcgggtccga
     7921 ccatcttgcc ctgcgcggcc tgatgacgat cgcgcccctt gtcgacgatt cggaggaggt
     7981 ccggccggtc tttcggcgac tccgggagat ccggagtcgc gtgcaggacg actccggact
     8041 caaattgcca ctcctttcga tgggcatgac caacgacttc ccggtcgcga tcgaggaggg
     8101 cgccacgcac gttcggattg gcagggccat cttcggctag caggagcgca aatggacgcc
     8161 gagctctgga caacggtcga tgcctggttc accgagcggc tgatagagtc cgacgtgggg
     8221 ctcgagcacg ccctggccga gatcgaccgc gccggcctgc gccagatcag cgtgtcgcac
     8281 aaccagggca agctgcttta tctcctgacc cgcatgaccg gggccaggcg aatccttgaa
     8341 gttggcacgc tgggtggata cagcgcaatc tggtttgccc gcgggttgcc cgaggatggg
     8401 aggctgatca cgctcgagat tgacccgaaa actgcagagg tcgcgcgcgc caatctgacc
     8461 aatgcaggcg tcgatcacct cgtggaagtg cgaatcgggg cagccctgga gtcgttccag
     8521 gcaatcgcgg acgaagagca tgacccgttc gacattgtct tcatcgacgc cgacaaggag
     8581 aacaacccag cctacttcga gtgggcactc aagctcacgc accccggcag cctgatcctc
     8641 atcgacaacg tcgcgcgccg gggccgcgtg gtcgacttaa gcgaatcgga gccggacatc
     8701 atgggcgtcc ggcgcgtcgt cgatatgatc gccaatgagc cgcgcgtgac cgcgaccgcg
     8761 attcagaccg tcggcgaaaa gggctatgac ggcttcctga tcgcgctcgt caccgcatga
     8821 tcacggcgcc ggcgtcgcaa cgatcggctg attagcggtc ggcgtcgcct gtccaccggt
     8881 cagcgtctcc atctggtgga gaatcgacgc cagctcggcc tgcgcttgcc cataggccgc
     8941 ccaatcgccc cgcgccaggg catcctgact tcgctggtag gcatcgtttg cttgctgaac
     9001 cagcgcctgc atgtcgccgg aagcaacggg ctcccctccc ccgccttcgg tgccaggggt
     9061 gacgctcgac tcctgacccg tcagcgcacc cgcgagcgcc ttgtcgagcg tgtcggccat
     9121 gaacacgttc tgctccgtcg cgacgatcac cctgcgcaac tccgggatgc tcgacccgct
     9181 ggtagacctc aggtagagcg gttggacata gagcaccgtc tgcttgaccg ggatgatcag
     9241 gagcgcgccc tgaagcacct gcgagcccga ttggttccag aggctgattt gctgtgagat
     9301 agccggatcc tgatcgatcc gcgcttcgac ctgggccgga ccgaacacat tcgtctggcg
     9361 tgggaatcgg tagaccgaga gggtcaggct gccatccggt cccgatcgac cagccatcca
     9421 tgcggtcatg ttcttgcgga cggagttgcc acccggcacg aatggccgca ccagggtgaa
     9481 cttcgcgttc gtctcgccgg gcagcggaac cgtaatgtag tacgccggca tctcgtcccg
     9541 ctcggatttg ttctcgagca ggtcctccga cgccagactt cctgggaccg tccagcgatc
     9601 ctcgccgttg tagtactggt cgggatcgtc gacatggatc gcgccgtaaa tgcgcgattg
     9661 cacatcgtaa atcggctccg ggtagcggaa atgggcggca accgtggccg gcgcggtcga
     9721 gatcggctcg aacagcgacg ggaacattcg ctcgtaggcg tcggcgatcg gatccagctc
     9781 ggcggtccgg tagaacgtgg tcgcgccggt gcgggcgtcg accgtgatct tgacgctggc
     9841 ccggatgtag ttgatgccgt cctgcggcgt cgacatcggg aacttcgacg acgtcgtgaa
     9901 ggcatcaacc acccagacca gttggccgtc cgcgatcacc aggtacgggt ccgggtcgag
     9961 cgtcaggaag ggcgcgatca acttgatgcg ttcctcgatg ttccggttca ggagcagggt
    10021 cgagctgtcc tggatgttgc ccgagatgaa gacgttgcgg tccttgagcg cgagggcgct
    10081 gatcagcttg gtcaccgagt cgtcgagcct gatcccgccg acgatctcat cctgcgtcaa
    10141 cgacgtcgtc tggtcgacct ccggcaatgc cgagaactcc tgcaccttgg tgccgacgat
    10201 cacccagccg aggtccgcct caccgtaata aatctccgga tgcgtaatcg tgaatggacc
    10261 gcttccgttc ggcggaattt ggctgacggt gaagaccggc aggccgctct gggttgcctc
    10321 atcgaccggg ctgaccaccg cgccatagcc atgcgtgtac acgaggcgac gattggtcca
    10381 ggtcagggcg ttcgacggca atccatcctg gttcagctcc ctggccgaca cgagcaccgg
    10441 ctgctcgaca ccgttgacca cataccgatc aatgtcgacg tcgaggtact ggtaatacgg
    10501 cgcgtacgac gtcaactcct ggtaggtcgt ccgcgcgacc cggtagtccc acaggcgcac
    10561 gttcgagacc agctcgggat tctcggcgat gtcctgcgag gtgatcgtgc cggtgccggc
    10621 caactcgccc tcctggacat ccccgaggcc gtaggcataa ctggtcattg cgatgttgtt
    10681 cgcgatgtac gggcgctcct tattcaattc gtcaggctcg acgatcgcct gcttgacgac
    10741 cagtggcagg agaccctgca cgccaatcga caggaagatc acgaccaacg ccgccaggaa
    10801 cgcctcgcgt cgctttggta cccgccggct ggcgatgcag gccgcgccgg ccacgatcaa
    10861 cgcgatgatc gcgacccaat tggcgattcg gatcgcatgg tcatccgtgt agctgacccc
    10921 atatgccgcg cctcgtgtcg aatagaccag gtggaagttg tccagataga accagatcga
    10981 gcccagcagc aaaagcacgc cgatcaaggc aagcagatgg cggaaagcgg ttgccggcaa
    11041 cttgcccacg ttgcgcaagt tcaatcccag ccggatcagg tagatgaacg cggtcaacac
    11101 gaatgcgatc agcgtcgcga tcgccgcgac cttccagatc agatcgagcg caggcaacgc
    11161 gaacaggtag aagctggcat cgcggtggta gatgggatcc ttgatgccca tcgaccgccc
    11221 gttccaccag agcgcgaagg tctcccaccg gttggcagtc caccaggcga gcccgacgcc
    11281 tgtcacgacc gacacagcca cggcaatcca gaacagcagt cgattggcaa aacgcacgaa
    11341 ggtcgatcgc gagacttctt gccgcgtccg ccgaaacgcc aacgacgcag atccgccgac
    11401 gatcccgaac ccgatcagcg cgaccaccag gaaggccgca acctggagcg ccgcgcgccg
    11461 gagcaggacg ctgcggtaac ccatactgtt gaaccaccac cagttcatcc agaaccgggc
    11521 agagacgaat accgcgacga tgacccccac gatgatgagc gcggcgatga ttcgccgctt
    11581 gtttgcggga gaaatcgtga ttttggggat gactggtgtg cgaggtcgac gcggtgtccc
    11641 ggtcggtttg gcggcaaccc gtcgaaactg tcggaacatg gaaggtgcat aaactcctgg
    11701 tgaacccgca tcgcgctgcc gcaaagcgta ctattcagac aatgaattcg accgagccga
    11761 tcctccagtc aaacgcaccg atacgcatcc gcagcctcgc cagcggcagt tctggcaacg
    11821 cgtacctggt cgaatccggc gacgatctgg ttctgattga ttgcggaatc gggattcgcc
    11881 agattcaggc cgcccttcat gcctggggca agacactccg cgacctgtcc gccatcgtca
    11941 tctcgcacga gcattccgac cacgtgcgat cgctggcgtc gtttctgcga cagaggacgc
    12001 cgatctacgc caccggtggc acgtccgacg cgcttgggct gagctccagt tgcctttcga
    12061 cgatccggta cacggagtcg ttcgacatcg gctgcatggc aatcacgccg atcgccacct
    12121 cgcacgatgc cgctgagcca tgcgggctgc tgatcgaagc atccgagcgg acgatcgggc
    12181 tcattaccga cctcggaacc gtgaccgacg agattggcac ctttgcgagc cggtgcgaat
    12241 tgctgatcct cgagtcgaat cacgatcgcg acatgctccg ctccggcccg tatccggccc
    12301 acttgaagaa acgcgtggca ggcaacttcg ggcacctgtc caatgatcag gccggcggtt
    12361 tcctggccga gatcgcgacc gctacgtcag gcccgagcga aatttggctc gctcacctct
    12421 ccgcgaccaa caacacgccg accgtagcca gggccgcgat ccaggaccgc ttccgcaagg
    12481 caggcgtctc gccggcaatc acagtcctgc ctcgcggtgc gcccggacca atctgggacg
    12541 gcgcgatcgt gcgggccaaa cagttggcgc tcatttccga cttctgacgc gggccgaaac
    12601 cacaggagct gaaacccctg gacacgatac tgacgattca cgatcgcctc tcgatcaccg
    12661 tgctgctttt catggccgcg gtcgggatct ggggaatgtt cacgtttatg cgcggcggcg
    12721 tgatggaggg gagcatcagc ggcagcctgg ttattgggca ggtcctgatc ggcgtgcagg
    12781 ggctgatggg cctcattatg ttcgccgatg gattccggcc ggcggactcg gtccacatcc
    12841 tctacgggat cgcagcgttc gtcacgatgc ccttcgtctg gtcgtatgcg cgcgagcggc
    12901 atccgcgtca aggcctcttc ttctacagcc tcgccgcgct gtttattgcc ggcctcgcga
    12961 tccgcggcat cacgaccggc ggcgggtgat tttcgtctca cccgtgagtc ctgatccaga
    13021 atgtgcaagt tcgcacaatt attgacacca cttgcgcccg cgcgtacaat ctgaccgtac
    13081 aaggactacg tacgcccccg cacgtgaata agggctgcaa taaggagccg tcgtggatac
    13141 gcaagcgcgg gactaccagg aagtcatcga tcaagccctg ggattggtct acagccatca
    13201 caaccgactc atcacgcggc tcgcgccgtc gacgttcgta ccccttgaca tcgaagacct
    13261 gagagagggt ccgctgggcc gcgacctttt gcgcctggct aacctcgccc gcgggcagac
    13321 ccgggaggaa aaggagctcg tccttcgcgc gatcgaagac gtggtccaac tcctgttctg
    13381 gccggcggca gccgacgagt acaccgttcc acgctcattc tgggataccg acctcggccg
    13441 gatgatggca ctggcgaagt tccgcgccta cgagccgcat gaactggtca gcatcggtgt
    13501 ggccgccgag acccttcagg tcacccggcc gacgatatac cgctggatgg atgaccggcg
    13561 cttgaactac gtcaaggacg agatcagcgg gcgcaccttc atcgtccgcc gcgacctcga
    13621 caactggaat acggtcgggt cggacctgga cgtagaggcc agctccgtct tctaggtaac
    13681 agacacacgc tgcggccggg gtatttatcc cggccgcttt cgtttgctca taccgtctcg
    13741 aggcggttct cgatcagcgt cgcgacgacg cgaccgttcg tcacgtaata gcgcggaacc
    13801 ct
//
LOCUS       DATAOZ010000410         3915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2274119, whole genome shotgun sequence.
ACCESSION   DATAOZ010000410 DATAOZ010000000
VERSION     DATAOZ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274119"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VHR64_10490"
     CDS             <1..529
                     /locus_tag="VHR64_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3303299.1"
                     /translation="VFQSFNLFPHMTALKNVTFGPMKVLHEPKADVERRARELLERVG
                     LADKADAYPAQLSGGQQQRVAIARAVAMNPKVMLFDEVTSALDPELVGEVLAAMKQLA
                     ETHGVTMIVVTHEMAFARDVADRVVFMDQGVVVEQGPPDQLFTNPKSDRLRAFLARFA
                     GSHATPLPVATAAAT"
     gene            697..942
                     /locus_tag="VHR64_10495"
     CDS             697..942
                     /locus_tag="VHR64_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303300.1"
                     /translation="MTDQDPKTQLGYRLYWKRIALHLNLRDIAGELGTTSVALSNFEK
                     GDFTRLTREQLLGYLDKLEMSNKADDYLALMPEALEA"
     gene            1044..>3915
                     /locus_tag="VHR64_10500"
     CDS             1044..>3915
                     /locus_tag="VHR64_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3303301.1"
                     /translation="MKSRLTSFRRLRSAIAMMIAAVLMTTVSFLDGPVAQAAVPGGFT
                     DSAVMSVSAPTDIAFLPDGKMLVTSQGGRLYMRSGSTTTLLRDLSGSICADFERGLLG
                     VTADPSFTSNGYIYLFYTYKKFGTCVDNSSNSPVNRVSRFVLSGTSVSGEKVLVDNMP
                     SPNGNHNAGGLLFGKDGYLYISIGDGGCEIGSPSECAGDNDNARVTNRLQGKVLRITR
                     DGNVPSSNPFISNGGNCRAPENNGRTPATYCEETYGWGFRNPFRIALDPNASGTRIFV
                     NDVGQGMWEEIDQLSAGKDYGWNFCEGTHATGSSSTCGFADTNPIFEYGHGSCNSITG
                     GAFIPNGSWPSTYDNAYFFADYTCGTIWMLKNSGGTWSRTTFASGLGAVTELEFGPYN
                     SNVALYYADYGASQIRRISYTAASNRAPVAKATASPTFGGVPLTVAFDGRQSSDPDSD
                     TLTYRWDFGDGTSSTRSTFSKTYQQAGKYRATLTVSDGRGGTNTDSVTIQAGNTRPSA
                     EITSPSASAEFNVGQTITLTGKGTDAEDGAIPASRMTWQVLLHHDTHTHPLFGPTTGN
                     NLTFQAPAPEDLAAAATSYLEIRLTVRDSGGLGKVAALNLMPNKVNVTVGSNVPGMLL
                     WIEGAPFTAPATVLTWEGNPLSVMAPDQIGPDNQPYTYKSWSDGGRRSHNYIAPASDA
                     TVTATFNLLPGDSFAPHADARVSEANPTRNEGTSTGLSVKGGTSSDYETVIRFKLSGI
                     GDRVYRARLYLYAYDPTVDGPSIYETSPSWSETGVTWNTRPAPIGGQLGNYGPITDNS
                     WIAYDVAAAIDGNGYVAFVIRGSSSDSVSWYSRQAGSKQPRLVVWSSEVAASEVMPEP
                     SGTPIPQASPAESPTPESTESPTPVATPDLGSPTASPEPIDEIPFADAFENDFSAWSG
                     DGAQLAPGTGPDGSQAVTLQSAGSANQPGAASYIRRDLPEGES"
BASE COUNT          754 a         1339 c         1177 g          645 t
ORIGIN      
        1 ggttttccag agcttcaacc tcttccctca catgaccgca ctcaagaacg tcacgttcgg
       61 cccaatgaag gtcctccacg agccgaaggc ggacgttgag cgccgagcac gcgagctgct
      121 cgaacgcgtc ggcctcgctg acaaggccga cgcctacccg gcacagctct cgggtggaca
      181 gcagcagcgc gtcgcgatcg cccgcgccgt ggccatgaac ccaaaggtga tgctcttcga
      241 cgaggtcacc tccgccctcg atcccgagct ggtcggcgaa gtgctggccg cgatgaagca
      301 actcgccgaa acgcacggcg tgacgatgat cgtcgtgacg cacgagatgg ccttcgcccg
      361 cgacgtggcg gaccgggtcg tcttcatgga ccagggagtc gtggtcgagc agggaccgcc
      421 cgaccagctc ttcaccaacc cgaagagtga tcgccttcgg gccttcctcg cccgcttcgc
      481 cggcagccac gccaccccac tgccggtcgc cacggccgcc gccacctgaa tcgatcgagg
      541 ttgactagct cttcccgacc tatgaccttc ggaccgagac aaccgtgcgc gagtatgctt
      601 gaatcagaac atgatgcggg agcggcgcat gtgatcgaga cccgcgctca cgccgccccc
      661 acagccgaat tcattgacga ttccggagta ccccgcatga ccgaccagga cccaaaaacg
      721 caacttggct atcgactcta ctggaagcgc atcgctctgc acctcaactt gcgcgatatc
      781 gcgggtgagc tgggtacgac ctccgtcgcg ctcagcaatt tcgagaaggg cgacttcacc
      841 cgcctgacgc gcgagcaact gctcggctat ctcgataagc tcgagatgag caacaaggcg
      901 gacgattacc tcgccctgat gccggaagca ctcgaggcat agcccacagg gcatagtgac
      961 gccggttccc taaacccgta gactccccaa aacgacaaaa acttcacctg catgtgacat
     1021 gcggtagggg ctggggagcc gcaatgaaga gccgactcac gtctttccgt cgactgcgca
     1081 gcgcgatcgc gatgatgatc gcggctgtcc tgatgaccac ggtctcgttc ctggatggtc
     1141 cggtggcgca ggctgccgtg cccggcggat tcaccgattc cgccgtgatg tcggtcagcg
     1201 ctccgacgga cattgccttc ctgccggacg gcaagatgct ggtcacaagc cagggcggcc
     1261 gtctctacat gcggagcggg agcaccacga cgctgctgcg ggacttgtcc ggctcaatct
     1321 gcgccgactt cgaacgcggc ctgctcggcg ttacggccga tccgtcgttc acctcgaacg
     1381 gctacatcta cctcttctac acgtacaaga agttcgggac ctgcgtcgac aactcgagca
     1441 actcgccggt caatcgcgtc tcccgcttcg tcctgagcgg aacatcggtc agcggcgaga
     1501 aggtcctggt ggataacatg ccgtcgccga acggcaacca caacgccggc ggcctgctct
     1561 tcggcaagga tggttacctc tacatctcga tcggcgacgg tggttgcgag attggctcgc
     1621 ccagcgaatg cgctggcgat aacgacaatg cccgcgtcac caaccgattg cagggcaaag
     1681 tccttcgcat tactcgagat ggaaacgtcc catcctctaa tccattcatc agcaacggcg
     1741 gaaactgtcg tgctcccgaa aacaacggca gaacgcctgc cacgtattgc gaggagacct
     1801 acggctgggg attccgcaac ccgttccgga tcgcgctcga cccgaatgcc tcggggacgc
     1861 ggatcttcgt caacgacgtc ggccagggca tgtgggagga gatcgaccaa ctctcggctg
     1921 gcaaggacta cggctggaac ttctgcgaag gcactcatgc caccggctca tcaagcacct
     1981 gcggcttcgc cgatacgaac ccgatcttcg agtacggtca tggctcctgc aactcgatca
     2041 ccgggggcgc gttcatcccg aacggctcct ggccgtccac ctacgacaat gcctacttct
     2101 ttgccgacta tacctgcggc acgatctgga tgcttaagaa ctcgggcggg acgtggtcgc
     2161 ggacgacctt tgccagcggc ctcggcgcgg tcactgagct tgagttcggc ccgtacaaca
     2221 gtaacgtcgc cctctattac gccgactacg gcgccagcca gatccggcgc atctcataca
     2281 cggccgcaag caaccgggcg ccggtcgcca aggcgacggc ctcgcccacc ttcggcggcg
     2341 taccgctgac cgtcgcattc gatgggcgcc aaagctccga tcccgacagc gacacgctga
     2401 cctatcgctg ggacttcggc gacggcacca gctcgacgcg ctcgaccttc agcaagacgt
     2461 accagcaggc cggcaagtac cgcgccaccc tgaccgtgtc cgacggcagg ggcggcacga
     2521 acaccgactc cgtcacgatc caggccggta acacccggcc gagcgcggaa atcacttcgc
     2581 cgtccgcctc cgcggaattc aacgttggcc agacgatcac gctgactggc aagggaaccg
     2641 atgccgaaga tggcgcgatc cccgcaagtc gcatgacgtg gcaggtgctc ctccaccacg
     2701 acacccacac gcatccgctc ttcggaccga ccacaggcaa caacctgacc ttccaggcgc
     2761 ccgctccgga ggatctggcg gcggccgcaa ccagctacct cgagatccgg ctgaccgtgc
     2821 gcgattccgg cggactcggc aaggtggccg cgctgaatct gatgccgaac aaggtcaatg
     2881 tcactgtcgg ctcgaacgtg ccgggcatgc tgctctggat cgagggcgct ccgttcaccg
     2941 cgccggccac ggtgctgacc tgggagggca acccgctgtc cgtgatggcg ccggatcaga
     3001 tcggaccgga caaccagcca tacacctata agtcgtggtc ggacggcgga cgcagatcgc
     3061 ataactacat cgcgccggcg tcggacgcga cggtcacggc cacctttaac ctgctgcccg
     3121 gcgattcgtt cgctccgcac gccgacgccc gcgtctccga ggcgaatccg acccgcaacg
     3181 agggaaccag cacggggtta agcgtcaagg gcgggacatc ctccgattac gagacggtca
     3241 tccgcttcaa gctgagcggt atcggcgacc gggtctaccg cgcgcgcctc tacctgtacg
     3301 cgtacgatcc gacggtcgat ggtccgtcga tctatgagac gagtccgagc tggagcgaaa
     3361 ccggcgtgac ctggaacacg cgcccggctc cgatcggtgg ccagcttggc aactacggac
     3421 cgatcaccga caactcgtgg atcgcgtatg acgtggccgc ggcgatcgat ggcaacggtt
     3481 acgtcgcgtt cgtgattcgc ggctcatcgt ccgactcggt ctcgtggtac tcgcggcagg
     3541 cagggtcgaa gcagccacgt ctcgtcgtct ggtcgagcga ggtcgcagcc agcgaagtca
     3601 tgcctgagcc gtcgggcacc ccgatcccgc aggcttcgcc ggcggaatcc ccgacacctg
     3661 agtcgaccga gagtccgacc ccggtcgcca cgccggacct cggctcgccg acggcatcgc
     3721 cggagccgat cgacgagatc cccttcgcgg atgccttcga gaacgacttc tcggcatggt
     3781 cgggagacgg agcccagctt gcgccaggca ccggtccgga cggctcgcag gcggtgacgt
     3841 tgcagtccgc tggcagcgcg aatcagcccg gcgcggcgtc gtacatccgg cgggatcttc
     3901 cggagggcga gtcga
//
LOCUS       DATAOZ010000411        19161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6744388, whole genome shotgun sequence.
ACCESSION   DATAOZ010000411 DATAOZ010000000
VERSION     DATAOZ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 19161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19161
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6744388"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1537
                     /locus_tag="VHR64_10505"
     CDS             281..1537
                     /locus_tag="VHR64_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303302.1"
                     /translation="MNRLILIVFAFVLAAQPSLAQEAIVLSPRAVDEGLAITEVARDL
                     AFPMGMTPLPDGSLLFATSPSTGGNFYDSTGELVRLFDSDGDGTLDDRHALISDLPGS
                     LVAVTRHGNIVIATSAQGGHEQIMFFRRGEHWRDPLTQIGAIQFIFRNAMHQSYALAT
                     RPNPDDPNSFDLVFNIGAAGNDTAGPKVQVTGGISGTLSSASLYMVTVTPASDGLTFG
                     EPVQIASGLRNGTTLAFAPATGDLWIGENGIDGLVNPIEAFSADELDVVPADQIGVKV
                     VDFGFPDSYVDMASGEIVGADTAAVSFRPIDGSEAEGIAGIVFVPESFPKELAGGILA
                     GFHGQFDMTGIENEENPVRWVNIATGDQFDLISNDSPGVGHLDSMTATSDGVYLADFC
                     NASMTSSRTCGVIYRLGVDSGKSDQV"
     gene            1590..1811
                     /locus_tag="VHR64_10510"
     CDS             1590..1811
                     /locus_tag="VHR64_10510"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX3303303.1"
                     /translation="MPSDESLTGEGNVAGIGKGLRNMVGILWIVLVVLLVLWFLGFIA
                     SVGGGFIHLLLVLALIVLIYNLLTGRRAV"
     gene            complement(1815..3413)
                     /locus_tag="VHR64_10515"
     CDS             complement(1815..3413)
                     /locus_tag="VHR64_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3303304.1"
                     /translation="MGERQADIAIVGGGLGGCAAALAALGLGKQVVMTEETDWLGGQL
                     TAQAVPPDEHPWIEQVGCTPSYRRLRDGIRDYYRRHYPLIPPAREDPFLNPGQAEVSP
                     ICHEPRVGLAVLEAMLQPYLSSGQLEVLIQHQPTAADVNGDRVRSVACRSLVTGEEVV
                     LHAPYILDATEFGDLLDLADVEHVIGSECQDETGEPHALPGPSQPLDQQAISWCYALD
                     YRPGEDHTIERPEDYDFWSTYAADFWPGAQLSWTDWHPETLEKRYRPIFDGPMERPRA
                     DDLWHFRRIRFNGHYPPGEIGSDISLVNWPQIDYWLGPVVGVTEDERQRHLRGARQLA
                     LSMLYWMQTEAPRLEGGYGYPGLRLRPDVVGTEDGLAKHVYVRESRRILSEFTILEQH
                     VGVEARHPIVGAEPFADSVGIGSYRIDLHPSTGLRNYVDVSSYPFQIPLGALIPQRVE
                     NLLPAGKNIGTTHITNGCYRLHPVEWNIGEAAGALAACCLEWDLPPRQIRNDETSLAD
                     FQRLLTSRFGLDLSWPEEIRGIAR"
     gene            3470..4414
                     /locus_tag="VHR64_10520"
     CDS             3470..4414
                     /locus_tag="VHR64_10520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX3303305.1"
                     /translation="MNRRTLLTAAFVLPVSGVRAQETAPRFQYPISLPDELPGDGFFI
                     RHGYACENTWFSPGNLHTGEDWYAIEGETAGAEIHAIAAGTVVFAESDYPGRVVIIEH
                     EPDLFSMYGHLDYALEVAEGDRVEMGQLIGTVLHRTDGAAPSHLHFEVRAFLTSPEIN
                     GDRPRYGFACGVNCPPGPGYWPIDAPEHPSAMGWRNPTHVIARRMYAGGDPPDGASVI
                     VPIEFDATVEAWSLPADRMAAERTGTMRLTAGDEYPLRNIAAGPEASSGWSAERYRLW
                     YQIDADAPVWIQAATPSAFETGGDGRPSTIQFAQIPAE"
     gene            complement(4854..5171)
                     /locus_tag="VHR64_10525"
     CDS             complement(4854..5171)
                     /locus_tag="VHR64_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303306.1"
                     /translation="MVTVRGDEDVAFLTAELLRDVADELWRDGIEMRFTFGHEHGMNP
                     VIDAWYRLNPGRVAVVECDGFGLSAGTSDCLATTIRERLFAAASLPEDRQTWDHTGTL
                     WPD"
     gene            5602..6660
                     /locus_tag="VHR64_10530"
     CDS             5602..6660
                     /locus_tag="VHR64_10530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3303307.1"
                     /translation="MAAIEIGLFDIQQVDPLSEADDQAIFAARLDDLALADQLGFQIA
                     FVAERHYLTTYRCQASSVWLGAASQRTSQIRLGVLAHTLPITPPVRLAEEIALLDQLT
                     GGRIEVGVGLGHRAEELAANHVDPADRITIFQERLAIMEGLWAGGTISYESEHSKVTD
                     VFIHPTTVQRPHPPLWFAGTQANAAMWAGQHGMNLAVGFAPSDKLFGATAAFRHGIGI
                     RQTRNPPDDAIRRGQIALMRQVYIGESTDQVRMEMTADLMRLGELNQSATAENRIDRK
                     HEAAEQYERLIAENIFIAGEPESVAREIVDARNKLGSSLILANVYAAGIEQERVRRTM
                     TLLAGPVREAIDHLTTRI"
     gene            complement(6722..7027)
                     /locus_tag="VHR64_10535"
     CDS             complement(6722..7027)
                     /locus_tag="VHR64_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303308.1"
                     /translation="MEAPLVLMAEPRTALGKKNGAIRREGKVPGVVYGPSYEQTVQVQ
                     VDRREFERFYLNTGLNKPFTLKWDGGQARVVMRDVQMNHLGTTAVHVDFYDGRAKKK"
     gene            7242..8189
                     /locus_tag="VHR64_10540"
     CDS             7242..8189
                     /locus_tag="VHR64_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303309.1"
                     /translation="MMFNYLVRRLIISAVTLVVISMVIFGILKIAPGDPLAGFANNPN
                     VPIETRARIRKNMGLDDPVPVQYAKWAREYIRGDWGQSFLSRAPAREVIFERLPVTLK
                     IIGTAYLLSVLIAVPVGVFSAIKQYSAFDQISTTFAFLGYSLPTFFSGLVLIVIFSFK
                     LDWFPFVYDKTVQGFWPNVKQSILPVTVLGLAGSAELTRFVRASMLEVISQDYVRTAR
                     AKGLREQSVIILHAMRNAMIPVVTILALQIPAIFGGAIITEQIFRIPGIGSLLIESIN
                     SADHPVVMGISMGIAILVVLFNIIADVVYALLDPRIKLA"
     gene            8205..9188
                     /locus_tag="VHR64_10545"
     CDS             8205..9188
                     /locus_tag="VHR64_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303310.1"
                     /translation="MVQHVSTAPATTAPGAAALDQLEKAGLKKHRSLWNNAWRQFRHH
                     RLALAGLIVFVSLVLITFVGAMVYPRDKDYIDFMVTSLPPFQSWKYPLGTDTLGRDIL
                     ARLLWGGRVSIAVGVISAGVAITIGTLVGAIAGFFGGFADSLLMRVTDLFISLPQVPL
                     LLMVSFLFRDSFYNWFDREFNNGNLGVFVLIVLVLAALSWMPTARLVRASFLSLKEKE
                     FVEAARAVGVNRPALIMRHILPNSLSPIIVAATLSVGAAIIGESVLSFLGLGFPSDVP
                     TWGRMLFEAKDFLQLSPHEAMIPGTAIFIIVLSINYIGDGLRDALDPRKTQ"
     gene            9188..9523
                     /locus_tag="VHR64_10550"
     CDS             9188..9523
                     /locus_tag="VHR64_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303311.1"
                     /translation="MITQDHQFHLETVTDPVFDWKDHSRFVEIFPVSTGWLVLWGKYE
                     EMGAVRTLHGSRVYRSAEGARRRLAWAIVGLTRNPVDARDALRLLDHTGGLPSHRPRE
                     KRSVPSAGA"
     gene            9559..9957
                     /locus_tag="VHR64_10555"
     CDS             9559..9957
                     /locus_tag="VHR64_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX3303312.1"
                     /translation="MPKGPVPARYRDILESRAPGHLATIDPNGRLNVNPVWFLADGDH
                     FYLSIKPDTAKYRSLRTNPNVALSVSDPARPDRYVELRGSVVDFELFDNLSWVNQLSR
                     KHTGSDFTAGVDGEHRYKVTVRIDSWTAQN"
     gene            complement(10188..10859)
                     /locus_tag="VHR64_10560"
     CDS             complement(10188..10859)
                     /locus_tag="VHR64_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3303313.1"
                     /translation="MVSTAERTGTIRTDIRPAGTLARPAASPRVAVFASSPALRAGLT
                     ALLSDDERVVVLSDDALAGGESPDVILLESGASDLIGELIDDQWPRSALLFVGEPPNE
                     ALSSGDRLIGAVSAGIEGSRLSAAVQAMAAGLVVVDPELQAAPPLRATASDPPDQPDI
                     LTPRERQVLELVARGYPNKSIAYELGISEHTAKFHVGSLLTKLDAASRAEVVTNATRR
                     GLLSV"
     gene            complement(10852..11763)
                     /locus_tag="VHR64_10565"
     CDS             complement(10852..11763)
                     /locus_tag="VHR64_10565"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX3303314.1"
                     /translation="MSQKRSSALVDLSEAIAAVVSDARAYTVTVYGRKRLAATGISWT
                     EDLVVTANHVVERDDEIEVGTDGETRVAATVAGRDPAGDIAVLRITGTKLTAAPRTGE
                     ASKAGQFVLGIGRAGGGNPEVSMGIVNTAEAGLRVSRGRIIEPVIQSEIVMLPGFSGG
                     PLLNPYGDVLGMNSSHLGRGASLTVTTAALEPIVDALATHGRLRMGYIGVGAQAVGIS
                     ASQVAEAKLEREVGLVILSIDETGPAQSAGLLIGDILVSVNGAPVGAVDDLVDQLSGD
                     LIGKSIPITVVRGSQTQDLQITVGERG"
     gene            complement(11884..12573)
                     /locus_tag="VHR64_10570"
     CDS             complement(11884..12573)
                     /locus_tag="VHR64_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX3303315.1"
                     /translation="MDFGALVAIGKIIAFDLILSGDNAVVIGMAANRLSPENRRRAII
                     IGGAGAVILRIIFTALVSLLLDVPLLQAAGGILLIWIAYKLVKPHGHDEAHVQSADTL
                     MQAIQTIIVADAVMSLDNMLAVGAAAEGHLWLLLFGLGLSIPILLFGSALIARLLGRY
                     PILMWAGVIILLHTAVHMFFGDPYVEDALGHISQAVVWTVAIALSILFYAVIRFRFGR
                     RAAPREVVPSS"
     gene            complement(12688..13590)
                     /locus_tag="VHR64_10575"
     CDS             complement(12688..13590)
                     /locus_tag="VHR64_10575"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX3303316.1"
                     /translation="MLNVMPNPAGTTRAQSIVREYIALTKPGILSLLLFTTLCGMMVA
                     AKGAPSLALTIVTMLGGAMAAGGANTLNCYIDRDIDGIMGRTSHRATVTGRISARNVL
                     IFGLALSVGSIIVIGFGANWLAAGLALAGNLYYVLIYTLLLKRRTPQNIVIGGAAGAF
                     PPLVGWAAVTGNLTVLPVLMFAIIYYWTPPHFWALALLKQGEYGRAAVPMLPNIVGEA
                     ETRKQVLLYTVILAAISVMPVVFGLGEIYFASAVVLSGIFLLWSARLYFQPSKRLARE
                     TFFYSLWYLALIFGAMVVDRAVLG"
     gene            13784..15748
                     /locus_tag="VHR64_10580"
     CDS             13784..15748
                     /locus_tag="VHR64_10580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX3303317.1"
                     /translation="MAFGFTPHALVYDLFSVGDPQISPDGTRVIWLQSHAERNRLKPE
                     SQIWISDVDDYRSRQLTTGSRQHGSPRWSPNGDDIAFTSFRDGKHGIYTIRPSGGEAV
                     LQTTHPTPPGELAWAPHGIQLAFVAPYDPEYLDGPPDDPELAPPVRVVNRIDYKQENR
                     GFLNNVRNALWTVEIYTGDKQRLTDDLTDHLAPVWSPDSQLIATKISRRNGMTSQLCV
                     TDAVRGGTKAIIGPEDGFISTWIFTPDSKAILYSGDTTQSWQADWFIYSLETSETRRL
                     TDDLRVSVDGGFPTVALPAQPVWLNDDQVIYHGFSRGQSGIYRLTISTGANEELYSWP
                     AVHAGFSIDEERDYIVQVRSSPRTSGEVIQIAIDSGEIDVIDEPNAEYFYAQDIGPWE
                     RITIERGGLEMDGWLLLPPEFDSSKQYPLVLDIHGGPNSHHGPGFNSTQQALAGAGFA
                     VLFTNPRGSSSYGRAFTQAVVGDWAGEDYLDQMAFLDKICSRTYIDAERVGVYGYSYG
                     GFMTSWIVGHTDRFKAAVIGAPVVDLVSFYGTADIGHTFGPLQVGGTPWDCPEEYRAR
                     SPLTNLHKATTPSLIVHGEADDRVPIGQGEQCFMTLKQAGCVTEFVRYPGEAHAMLRT
                     GLPAHREDYHSRVVAWFETYLTDRGSAQDG"
     gene            15741..16355
                     /locus_tag="VHR64_10585"
     CDS             15741..16355
                     /locus_tag="VHR64_10585"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3303318.1"
                     /translation="MDEQTIEAYEQLAEAIFSRHTRFDRATHHERMLPWLRPGEPTAD
                     VGSGSGADLAWLVSQGFPAVGYEPVAGLRQLAIATFPGIDVRASSLPDLEGVPAGAFG
                     NVICSAVLMHLPVSSISPAIASLSRILRPGGRHLLSYRPGKDGAEREDDGRLFTPIAP
                     DLMNRFLSESELRVVHQSATADPNRPGVIFERFVAEKLQQVPDV"
     gene            complement(16504..17589)
                     /locus_tag="VHR64_10590"
     CDS             complement(16504..17589)
                     /locus_tag="VHR64_10590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HEX3303319.1"
                     /translation="MAGHKRWFRVQAIGTVLREDAAGPDAFLDPARPSIIRLDPRWKA
                     GLDGLEEFSHLVVLFYADRVERRRTAGEPMRPEGRDEMPAVGFFALRTPRRPNPIGVC
                     CPRLVRRDGTDLHVTGLDAWDGTPVLDIKGYYPRDEQRPDATVPGWLTTLWRKHDAER
                     AAEALTSPGPTMPTPGTVVGRHESAKGEVIFRYPLMTDAPSALKFVNALSAERTFVLF
                     QGERMTLAQEQAWIGERLAALAAGKGIPLFAFAGDRYIGSAVIELGSLVATHIGYFGI
                     GLASDFRDQGVGSALMHAVIDEAERYLAGMRMIQLDVFASNERGIHLYRKMGFTEYAR
                     LPGAVHHRGDYVDMISMYRPVNRNQTP"
     gene            17663..18118
                     /gene="rpiB"
                     /locus_tag="VHR64_10595"
     CDS             17663..18118
                     /gene="rpiB"
                     /locus_tag="VHR64_10595"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX3303320.1"
                     /translation="MHLAIGGDHAGFPLKGPIIDALRDWGHEVVDLGTHSTEPVDFPD
                     ITKAVCARVLNGEADRAILVCGTGVGACIAANKIDGIRAALCHDVYSAHQSVEHDDVN
                     VLCLGAQIVGLPLALDLLRAFLAARFSDEEHFRRRVQKIADLERGNLAL"
     gene            18115..18687
                     /locus_tag="VHR64_10600"
     CDS             18115..18687
                     /locus_tag="VHR64_10600"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX3303321.1"
                     /translation="MTAVDLQTAPDFAFMNADGNEVRLSDFWESAPKGIVLVFLRHFG
                     CLFCREHATQLRDHYAEFSKRGYQVVAVGQGTPARSAKFRTDYDLPFPVLGDREMASY
                     RLFGLTSGGMGGLIQPRSWVPGVRALLHGSRPGMLDGSLNQNPGTFLIDRNGRILRQQ
                     IGQHAGDFPSATEILGWIDSLHQAISAGFW"
     gene            18766..18924
                     /locus_tag="VHR64_10605"
     CDS             18766..18924
                     /locus_tag="VHR64_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303322.1"
                     /translation="MAAQTLSQVHQPNRFLDLYRHLVDLAIASVEMAFGDWRALPVDR
                     PPPSPARQ"
     gene            18964..>19161
                     /locus_tag="VHR64_10610"
     CDS             18964..>19161
                     /locus_tag="VHR64_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303323.1"
                     /translation="MVSTPVRSTKDLIDREKVQQLINREEQRLNANTEVSAQYYERAK
                     KHMVGGVPSSYQARDPWPIYLS"
BASE COUNT         3528 a         6349 c         5832 g         3452 t
ORIGIN      
        1 gacaaaccag gccatgagag ccgatggcaa ttatgcaaaa tacatccggg cccacaccgc
       61 agccctctga cgcgccgaaa accgctccac ccttgctcaa gatcccacct ccggcaccgc
      121 cctccatcat tcacgtgtcg ccaaatccgt ttccgtcgac taagggtgtc ttctcggatc
      181 cccatacgaa ccaaagaggc gccgacttcg tgacatcgta tgcttttggc gacgacaacg
      241 aggttgaagg caacgtgtgc cacgtggtca gaggagaagg atgaaccggc tgatcctgat
      301 cgtcttcgca tttgtattgg ctgctcagcc gtcactggca caggaggcca tcgtactgtc
      361 accgcgggct gtggacgagg ggctggccat cactgaggtg gcgcgggatc tggcgtttcc
      421 gatggggatg acgccgctgc ctgacggttc gctgctgttc gcgaccagcc cgtccacggg
      481 cggcaacttc tacgacagca ccggcgagct ggtgcggctc ttcgactcgg atggcgacgg
      541 cacgctcgat gaccggcacg cactgatctc ggacttgcct ggctcgctcg tcgccgtcac
      601 ccggcacggc aatatcgtga tcgcgaccag cgcccagggc ggtcacgagc agatcatgtt
      661 cttccgccgc ggcgagcact ggcgcgaccc gctgacccag atcggcgcga tccagttcat
      721 cttcaggaac gccatgcatc agagctacgc tctcgcgact cgtccgaatc cggacgaccc
      781 gaactccttc gacctcgtct tcaacatcgg cgcggcgggc aacgacaccg caggaccaaa
      841 agtgcaggtg actggaggta tctccggcac gctgagctct gcgtcgctct acatggtcac
      901 agtgacgcca gcaagtgacg gattgacgtt cggagagccg gtccagatcg cgtccggcct
      961 gcgcaacggc acgacgttgg catttgcgcc cgcgaccggc gatctctgga ttggcgagaa
     1021 cggcatcgat ggcctcgtca acccgatcga ggcgttcagc gccgacgagc tcgacgtcgt
     1081 cccggccgat cagatcgggg tgaaggtcgt cgacttcggg ttccctgaca gctacgtgga
     1141 catggcttca ggcgagatcg tcggagccga tacggcggcc gtctcgtttc ggccgatcga
     1201 cggtagtgag gccgagggca tagcgggcat cgtcttcgtg ccggagtcgt tcccgaagga
     1261 gctggccggc ggaatcctgg ccgggttcca cgggcagttc gacatgacgg ggatcgagaa
     1321 cgaggagaac ccggtacgct gggtcaacat cgcaaccggc gatcagttcg acctgatcag
     1381 caacgacagc cccggcgtcg gccacctcga ctcgatgaca gcaaccagtg acggcgtcta
     1441 cctcgccgac ttctgcaatg cctcgatgac cagctcccgg acctgcggtg tcatctaccg
     1501 gttaggcgtg gattcaggca aatctgacca ggtgtagtct cagtatctgt ccccgaactt
     1561 gtgcattcct accatatgct cgtcgctgga tgcccagcga cgagagtctg accggcgaag
     1621 gcaacgtcgc cggaatcgga aagggtctca ggaacatggt aggtattctc tggattgtcc
     1681 tcgttgttct gcttgttctc tggttcctcg gattcatcgc cagcgtcggc ggcgggttta
     1741 tccatctgct gctggtgctc gccctgatcg tgctgatcta caacctgttg accggccggc
     1801 gagcagtcta acggctacct ggcaattccc ctgatctcct ccggccagga gagatcgagc
     1861 ccgaagcggc ttgtcaggag tctctgaaag tcggcaaggc tggtctcgtc gtttcgaatc
     1921 tggcgcggcg gaagatccca ttccaggcag caggcggcaa gcgcgcctgc tgcctcgcct
     1981 atgttccact ccaccgggtg aagccgatag cacccattcg tgatgtgcgt cgtgccgatg
     2041 ttcttgccgg ccggcaacag gttctccacg cgctgcggga ttaatgcccc gagcgggatc
     2101 tggaacgggt agctgctgac atcgacgtag ttgcgcagtc cggtgctcgg atggagatcg
     2161 attcggtagc tgccgatgcc gacgctgtcc gcaaacggct ccgcgccgac gatgggatgc
     2221 cgggcctcga cgccgacatg ctgctcgagg atggtgaact cggacaggat ccggcgcgac
     2281 tcgcggacgt agacatgctt ggccaggcca tcctcagtgc cgacgacgtc cggtcgcaac
     2341 cgcagccccg ggtatccgta gccgccctcg agtcgaggcg cttcggtctg catccagtac
     2401 agcatcgaca acgcgagctg ccgggctccg cgcaggtgac gctgtcgctc gtcttcagtc
     2461 acaccgacga ccggcccgag ccagtaatcg atctgcggcc agttcaccag cgagatgtcg
     2521 ctcccgatct ccccgggcgg gtagtggcca ttgaaccgga tccgccggaa atgccagagg
     2581 tcgtccgcac gtggccgttc catcgggccg tcgaagatcg ggcgatagcg cttctcgagc
     2641 gtctccggat gccaatcggt ccagctcaat tgcgcgccgg gccagaaatc cgctgcgtag
     2701 gtgctccaga aatcgtagtc ctcgggacgc tcgatggtgt ggtcctcgcc aggccggtag
     2761 tcgagcgcgt agcaccatga gatcgcctgc tggtcgagcg gttggctcgg tccgggcagg
     2821 gcgtgcggct cgcccgtttc atcctggcat tcggagccga tgacgtgctc gacgtccgcg
     2881 aggtcgagca gatcgccgaa ctccgtcgcg tccaggatgt acggcgcgtg caggacaacc
     2941 tcctcaccag tcaccaagct ccggcaggca accgaacgca cccggtctcc gttgacatcc
     3001 gcggcagttg gctggtgctg gatcagcacc tccaactgac cgctgctgag gtacggctga
     3061 agcattgcct ccagtacagc cagcccgact cgcggctcgt ggcaaatcgg gctgacctca
     3121 gcctggcccg ggttgaggaa cggatcctca cgggcaggcg ggatcaatgg atagtgccgg
     3181 cggtagtagt cccgaatccc gtctcgcagc cgccggtagc tcggagtgca gccgacctgc
     3241 tcgatccacg gatgctcgtc gggcggcacg gcttgcgcgg tcaactggcc accgagccag
     3301 tcggtctctt cggtcatcac gacctgcttg ccaagcccga gcgccgcgag cgcagccgca
     3361 cagccaccca acccaccacc aacaatggca atgtccgctt gtcgctcgcc caatttcggc
     3421 tcccgtttct ccagcaatcc tggctcgcgc tcgtatcgta ggatgccgta tgaaccgccg
     3481 caccctcctc accgccgcgt ttgtcttgcc cgtgtcgggc gtgcgcgccc aggagaccgc
     3541 tccccgattt cagtacccga tcagtttgcc ggatgagtta ccgggagacg ggtttttcat
     3601 ccgccatggg tacgcctgcg agaacacatg gttcagtccg ggcaatctcc acaccggcga
     3661 agactggtac gcaatcgaag gcgagacggc cggcgcggaa atccacgcaa ttgcggccgg
     3721 aacggtcgtg tttgccgaga gcgactatcc gggccgcgtg gtcatcatcg agcacgaacc
     3781 cgacctgttc tcgatgtatg ggcatcttga ctatgcactg gaggtcgccg agggcgatcg
     3841 tgtcgagatg ggacagctca tcggcacggt cctccatcga accgatggcg ccgcgcccag
     3901 ccatcttcat ttcgaggtcc gcgcgtttct tacctcgccc gagatcaacg gcgatcggcc
     3961 gcggtacggc ttcgcatgcg gagtcaactg cccgcctggg cccggctact ggccaatcga
     4021 tgcacccgag catccgtcgg ccatgggctg gcgcaatccc acgcacgtca ttgccaggcg
     4081 catgtacgct ggtggcgatc cacctgatgg cgcgtcggtg attgtgccga tcgaattcga
     4141 cgcgacggtt gaggcctggt cgctaccggc tgaccgaatg gcagcggagc gcacaggcac
     4201 gatgaggctg acggccggcg acgaataccc gttgcgcaat attgcggccg gtccagaggc
     4261 aagcagcggc tggagcgcgg agcgatatcg gctctggtat cagattgatg ccgatgcgcc
     4321 ggtctggatc caggcggcaa caccatccgc cttcgagacc ggtggcgatg ggcgcccctc
     4381 aaccatccaa ttcgcgcaga tccccgctga atagcatgcg atatgggagc gccaactcct
     4441 catcggctgc gggccgactc gaggagtcgg tgcgttatct gaagcaaagg ctcgcgatcc
     4501 ttgaacgcgt ggccttctcc gcggcggatg gcctccgcca cgagtcggaa tgcttcgcgc
     4561 gatggcaacg tcgcaggcaa gtcatcccag aagagcgacg ccgaccggga gatcgcgaga
     4621 tcaaacggag acctatgtgt gtcccggcgc cagcgatcaa cggtctgttg cgcgcgtgcc
     4681 aggattgtca gatctggcga gatatcggcc cacacggtgc acctccttcc cgtggtgccg
     4741 cggtactggc ccaaacccta acataggtct tacgggaact ctgtaacatg tgcacatgca
     4801 aatagtcgcg tgggcgaccg gtcctgaagt cctcgtgtcg cgagaatccg gggtcaatcg
     4861 ggccagagag tgcccgtgtg gtcccaagtc tgccggtcct ccggcaggct cgcggcagca
     4921 aagagccgct cgcggattgt cgtcgccagg caatcgctgg tccctgcgga caagccgaat
     4981 ccgtcgcact cgaccaccgc gacgcgaccg ggattcaacc tgtaccaggc gtcgatgacg
     5041 gggttcatcc cgtgctcatg accgaacgtg aatcgcatct cgatgccgtc ccgccacaac
     5101 tcatccgcca catcgcgaag tagctccgca gtcaggaagg cgacatcttc gtcgccgcgc
     5161 accgtaacca tgatcaggac ctcgcattcg gcatggcaat gtttggccga acccgtactg
     5221 tagccttcgc cgggaagcac ggatgacgct gaactacgga cggttgccgg gaaccggccg
     5281 catcgaagac aaatgtggcg gaagtgaaac ggaccctggg ctcggtacta atgttcgttg
     5341 acgcttgcag cgcgagatgg tagtgttcac ctacgttgat ccgatcggat caaccagaag
     5401 ccaaccaaca ccgaacaggg tgaaggaggt gatgcccatg agtagtcacg agtttgaggg
     5461 catcacgccg gaggtcgctg gccgctaggc tatcgactga ggctgtgatg cccgagcaga
     5521 aggagccgga gtttaccggc tccttttgtt ttgcccgctg ctccgcggtc cgcgcatcct
     5581 caccccacat gaacggcgaa catggctgca attgagatcg gcctgtttga tatccagcaa
     5641 gtagatccgc tctcagaagc ggatgatcag gccatcttcg ccgcccggct ggatgacctc
     5701 gccttggccg atcagctcgg cttccagatc gccttcgtcg ccgagcgcca ctacctgact
     5761 acgtatcgct gccaggcctc gagcgtctgg ctcggcgccg cgtcgcagcg cacctcgcag
     5821 atacgcctgg gcgtcctcgc gcacacgctg ccaatcacgc cgcctgtgcg cctggccgag
     5881 gagatcgccc tgctcgatca gctgaccggc ggccggatcg aagttggggt cgggcttggt
     5941 catcgcgccg aggagttggc cgcaaatcac gtcgacccgg ccgatcggat cacgatcttc
     6001 caggagcgtc tggcgatcat ggaagggctc tgggcgggcg gcacgatctc ctacgagagc
     6061 gagcattcga aggtgaccga cgtcttcatc cacccgacca ccgtgcagcg gccacaccca
     6121 ccgctctggt tcgccggcac ccaggccaat gccgcaatgt gggccggtca gcacgggatg
     6181 aatctggcgg ttggtttcgc tccgagcgat aagctgttcg gcgcgaccgc ggcctttcgg
     6241 catggcatcg gcatccgcca aacacgaaat cctccggatg atgcgattcg acgcggacag
     6301 attgcgctga tgcggcaggt ttatatcggg gaatccactg accaggttcg catggaaatg
     6361 accgccgacc tgatgcggct cggtgagctt aaccagtcgg ccacggccga gaaccggatc
     6421 gaccgcaagc acgaggccgc cgagcagtac gagcgattga ttgccgagaa catcttcatc
     6481 gccggcgaac cggaatcggt cgcacgggag atcgtcgatg cgcgcaacaa gctcggctcg
     6541 agcctgattc tcgccaacgt ctatgccgcc ggcatcgagc aggagcgcgt ccgccggacc
     6601 atgacactcc tggcgggacc ggttcgggag gcaatcgatc acctgacgac ccgcatatga
     6661 cacagcaacg gccgctctcg agttaagagc ggccgttgct tgtttccggg ttagtgacgc
     6721 gctacttctt ctttgcgcgg ccgtcgtaga agtcgacatg caccgccgtc gtgcccagat
     6781 ggttcatctg cacgtcgcgc atgactacgc gagcctggcc gccatcccac ttgagggtaa
     6841 agggcttgtt caggcccgtg ttcaggtaaa agcgctcgaa ttcgcgccgg tcgacctgaa
     6901 cctggaccgt ctgttcatag ctcgggccgt agacgacgcc gggaaccttg ccttctctgc
     6961 gaatcgcgcc gttcttcttg cccagggcag tgcgcggttc ggccatgaga accaatggtg
     7021 cttccacctg attgactcct actacctaca ccaatcgtcc attccgcggg cctctgtgac
     7081 gagggcgcga aaaaacgcga cccgtgtcgc gtacgcatta actgtatcac gacagcgtaa
     7141 atacagggta gacggggtac gcatgtcgag agtatggttt cctatctcga ccttgagact
     7201 ggaaccacct tgatccgcta cgacagacga tgcggaggtg aatgatgttc aattacctgg
     7261 tccggcgtct gatcatctcc gcggtcacac tggtggtcat cagtatggtg atcttcggca
     7321 tcctgaagat cgcacccggt gaccctctag ccggcttcgc caataatccc aatgtcccga
     7381 tcgagacacg ggcgcgcatc cgcaagaaca tgggtctcga tgatccagtt ccggtccaat
     7441 acgcgaagtg ggcccgcgaa tacatcaggg gagactgggg tcagtcattc ctctcccgcg
     7501 ccccagcccg ggaggtcatc ttcgaacgcc tgccggtcac gctcaagatc atcggtacgg
     7561 cctatctcct cagcgtcctg atcgcggtcc cggtcggggt cttttccgca atcaagcagt
     7621 actcggcctt cgaccagatc tcgacaacct tcgccttcct cggctacagc ctgccgacct
     7681 ttttctcggg cctggtcctg attgtcatct tcagcttcaa gctcgactgg ttcccgttcg
     7741 tgtatgacaa gacagtgcag ggcttctggc ccaatgtgaa acaaagcatc ctgccagtca
     7801 ccgtgctggg tctggcaggc tccgccgaat tgacacggtt tgtccgcgcc tcgatgctcg
     7861 aggtgatcag ccaggactac gtgcgaaccg ccagggccaa ggggctgagg gagcaaagcg
     7921 tgatcatcct ccacgcaatg cgcaacgcca tgatcccggt cgtgacaatc ctcgccctgc
     7981 aaatcccggc catcttcggc ggtgcgatca tcaccgagca aatcttccgg atccccggca
     8041 tcggctccct gctgatcgaa tcgatcaaca gcgctgacca tccagtggtt atgggcatct
     8101 cgatgggcat tgcgatcctg gtggtgctct tcaacatcat tgccgacgtt gtctatgcgc
     8161 tgctcgatcc gcgcatcaag ctggcgtaac cggaggcgat gacaatggtc cagcatgttt
     8221 ccaccgcacc agctaccacc gcacccggcg ccgccgcact cgaccaactg gagaaggcgg
     8281 ggctcaagaa acaccgctcg ctctggaaca acgcctggcg ccagtttcgc catcaccgat
     8341 tggcgcttgc cggcctgatc gtgttcgtca gccttgtcct gatcaccttc gtaggcgcca
     8401 tggtctaccc gcgggacaag gactacatcg acttcatggt caccagcctc ccgccgttcc
     8461 agtcctggaa gtacccgctg ggaaccgaca cgctcgggcg tgacatcctc gcccggctgc
     8521 tctggggtgg acgcgtctcg attgcggtcg gcgtgatctc tgcgggtgtt gccatcacga
     8581 tcggcacact ggtcggcgcg atcgcgggat tcttcggtgg attcgccgac agcctcctga
     8641 tgcgggtcac cgacctcttc atctcactgc cgcaggtccc actcctcctg atggtctcat
     8701 ttctcttccg ggactcgttc tataactggt tcgaccggga gtttaacaat ggcaacctcg
     8761 gcgtttttgt cctgatcgtg ctcgtgctcg cagctctgag ctggatgccc actgcccggt
     8821 tagtccgggc cagcttcctt tcgctcaagg agaaggagtt cgtcgaggca gcccgcgcgg
     8881 taggagtgaa tcggccggcg cttatcatgc ggcacatcct gccgaactca ctcagcccaa
     8941 tcatcgtcgc tgccaccctg agcgttggcg cagcgatcat tggcgagtca gtgctctcgt
     9001 tcctcgggct cggcttcccg agcgacgtcc caacctgggg ccggatgctc ttcgaggcga
     9061 aggacttcct gcagttgtcc ccacacgagg cgatgatccc cggcacggca atcttcatca
     9121 tcgtgctctc gatcaactac atcggcgatg gtctgcgcga cgccctggat ccacgcaaga
     9181 cgcagtagtg atcacgcagg accaccagtt ccacctcgag acagtcactg atccggtctt
     9241 cgactggaag gaccatagcc gcttcgtcga gatatttccg gtctcgaccg gctggctggt
     9301 gctctgggga aagtatgagg agatgggcgc cgtgcggacc ttgcatggct cgcgcgtcta
     9361 ccgctcggcg gagggcgccc ggcggcggct ggcctgggcg atcgtcgggc tgacccggaa
     9421 cccggtagat gcccgcgatg cgctgcgcct gctcgaccac acgggtggcc tccccagcca
     9481 ccgcccgcga gagaaacgct ccgtaccctc agcaggtgcg tagatcgccg ttcatatcgc
     9541 aggagagcat atcccgaaat gcccaaagga cccgtcccgg cccgctatcg cgacatcctc
     9601 gagagcaggg ccccgggaca cctcgccacg atcgacccga atggccggct gaacgtcaat
     9661 ccggtctggt tcttggcgga cggtgaccac ttctacctga gcatcaagcc cgacacggcc
     9721 aagtatcgga gcctgcgcac caacccaaac gtggcgctgt cggtctcgga cccggctcga
     9781 ccagatcgct atgtcgaatt acggggttcg gtcgtcgact tcgagctctt cgacaacctt
     9841 tcgtgggtca accagctctc tcgcaaacac actggctccg atttcaccgc cggcgtcgac
     9901 ggcgaacacc gctacaaggt aaccgtccgc atcgactcct ggaccgccca gaactgagtt
     9961 cgctccgtcc gactgctacc gggacgggga taccctacgt tcgtcgtggc ggggctccgt
    10021 acccgccttg aagtgccggt cgcatcgttc acatcgattg cactgctgat caatgggtgg
    10081 gcaccaatgg ccgggttccg aggcaggtcc aggcggctac ggagccccgc cactacggca
    10141 gagagggacc ggatagggaa tgctccgctg ttacgggcgt atgcgcacta gacgctgagg
    10201 aggccgcggc gggtggcgtt ggtgacgact tcggcgcggc tggcggcgtc gagcttggtc
    10261 aggagtgaac cgacgtggaa tttagcggtg tgctcgctaa tccccaactc gtacgcgatc
    10321 gacttgttgg gatatccgcg tgcgaccagc tccagtacct gccgctcccg gggagtcagg
    10381 atgtcgggct gatcgggcgg atcgctggca gttgcacgca gtggcggcgc ggcctgcaac
    10441 tccggatcga ccaccaccaa tcctgctgcc atcgcctgca cggccgcgct caaccggctc
    10501 ccctcgatcc cggccgagac cgcgccgatc agccgatcgc ctgagctcag ggcctcattc
    10561 ggcggctcgc ccacgaacag caacgcgctt cgcggccact gatcgtcgat cagctcgccg
    10621 atcaggtcgc tcgccccgct ctccagcagg atgacgtccg gcgactcgcc accggccaga
    10681 gcatcgtccg acagcacaac gacccgctcg tcatcgctca gcagcgcggt caagccggcg
    10741 cgcagtgcgg gcgagctggc gaagacggcc acacgcggag acgcagccgg ccgggctagt
    10801 gtcccggccg gccgaatatc ggtgcgtatg gtgccggtgc gttcggctgt gctaaccacg
    10861 ctcacctaca gttatctgca gatcctgtgt ctgcgacccg cgcacgaccg taatcgggat
    10921 cgacttcccg atcagatcgc ccgagagctg atcgaccagg tcatcgaccg cgccgaccgg
    10981 cgcgccgttc actgacacca gaatatcccc gatcagcaac cccgccgact gggccggacc
    11041 agtttcgtcg atcgagagga tcacaaggcc gacttcgcgc tccagcttgg cctcggcaac
    11101 ctgactggcc gagatcccga ccgcctgcgc gccgactccg atgtagccca tccgcaaccg
    11161 gccgtgggtg gcgagggcgt cgacgatcgg ctcgagcgcc gcggtcgtga cagtcaggct
    11221 cgcgccccga ccgaggtgcg atgagttcat gcccaggacg tcgccgtacg ggttgagcag
    11281 tggcccgccc gaaaagccgg gcagcatcac gatctcggac tggatgaccg gctcgatgat
    11341 tcgcccgcgg ctgacgcgca atccggcctc ggccgtgttg acgataccca tcgagacctc
    11401 tggatttccg cccccggccc gtccgatccc gagcacaaac tggccggctt tggatgcctc
    11461 gccggtccgc ggggcggcag tcagcttggt cccggtgatg cgcagcaccg cgatgtcgcc
    11521 ggccggatcc cgcccggcaa cggtcgcggc gactcgggtc tcaccatccg tgccgacctc
    11581 gatctcgtca tcgcgctcga cgacgtggtt cgcggtgacg accagatcct cggtccagga
    11641 gatcccggtg gcggctaatc gcttgcgtcc gtaaacagtc acggtgtagg cgcgggcatc
    11701 ggatacgacg gcggcgatcg cctccgacag gtcgacgagg gcggacgaac gtttctggct
    11761 catgggtcag gctccagttc cgggacgacg accacgacac tcagcgtggc atgagccgag
    11821 tctcgatcag gctattggcc gatggtatcg ccggaatgga gagcgcagac actcgccgtt
    11881 tggctaggaa gagggaacga cctcgcgcgg cgccgcccgt cgcccgaagc ggaaccggat
    11941 cacggcgtag aacaagatcg acaacgcgat cgcgaccgtc cagaccactg cctggctgat
    12001 gtgcccgagg gcgtcttcaa catacggatc cccgaagaac atgtgcacgg ccgtgtggag
    12061 caggatgatc acgccggccc acatcaggat tgggtaccga cccaggagtc gcgcgatcag
    12121 ggcgctgccg aagagcagaa tcgggatcga caagccaagt ccgaacagca gcaaccagag
    12181 atgcccctcg gcggccgcgc cgaccgccag catgttgtcg aggctcatga ccgcgtcggc
    12241 cacgatgatc gtctggatcg cctgcatcag ggtgtcggct gattgaacat gcgcctcatc
    12301 atggccgtgc ggcttgacca acttataggc aatccagatc agcaggattc cgcctgcggc
    12361 ctgcaggagg gggacatcca gcaggagcga gaccagggcg gtgaagatga tccgcagaat
    12421 gaccgcaccc gcgccaccga tgatgatcgc ccgccgccgg ttttccggcg agagacggtt
    12481 ggcagccatc ccgatgacga cggcattgtc gccggacaag atcaggtcga aggcgatgat
    12541 cttgccgatt gctacgagag cgccgaaatc cacgaagccg gatccaatcc gtgatgtgag
    12601 gtcattgcca ggcccatcag ttcccgatga gccagatcgt caacgatacc cgaaccggga
    12661 cgcggcccgg gcagctcagg ctgcgttcta gccgagtacg gctcggtcca caaccatcgc
    12721 gccgaagatc agcgccaggt accagagcga gtagaagaag gtctctcgcg ccaatcgctt
    12781 gctgggctgg aaatacagcc gcgccgacca gagcaggaag attccgctca gcacgacggc
    12841 gctggcgaag tagatctcgc cgagcccgaa cacgaccggc attacagaga tcgcagccag
    12901 tatcaccgtg tagagtagga cctgcttgcg cgtctctgcc tcgccgacaa tgttgggcag
    12961 catcgggacg gccgcccgcc catattcgcc ctgcttgagc aacgccaaag cccagaagtg
    13021 cggcggcgtc cagtagtaga tgatcgcaaa catcaacacc ggcagaaccg tcaggttgcc
    13081 ggtcacggca gcccagccga ccagcggcgg gaaggcgccg gccgcgccgc cgatcacgat
    13141 gttctggggc gtgcgccgct tgagcagcaa ggtgtagatc agaacgtagt agaggttgcc
    13201 agccagcgcc agaccagcgg ccagccagtt cgcaccgaac ccgatcacga tgatcgaccc
    13261 aaccgagagc gcgaggccga agatcagaac attgcgtgcc gagatccgtc cggtcaccgt
    13321 cgcccggtga ctcgtccggc ccatgatgcc gtcgatatcg cggtcgatgt agcagttcag
    13381 cgtattcgcg ccgccggccg ccatcgcccc gcccaacatg gtcacgattg tgagtgcgag
    13441 cgacggcgcg cccttcgcgg cgaccatcat cccgcagagt gtcgtgaaga gcagcaggga
    13501 gagaatgccg ggcttggtga gtgcgatgta ctcccgcacg atcgattgcg cgcgggttgt
    13561 ccctgccggg ttgggcatca cattgagcac cgagccaaga acctttgtga agtgcggagt
    13621 cgtcgtggcg caactgccag cgctccgctc attctactct gccgccggtc aaaatcccga
    13681 cctccacgcc ccactttcgg catcaggttc cggtccaccc cgccccggat gcgcgtggta
    13741 ggctgccgac gcatgattcg gaccctcgaa aggagcgaac ctggtggcct ttggcttcac
    13801 gccccacgcc ctcgtctacg acctcttcag cgtcggggat ccccagatct caccggatgg
    13861 cacccgcgtc atctggctcc aatcccatgc cgagcgcaac cggctcaagc ccgagtcgca
    13921 gatctggatc tcggacgtcg acgactaccg cagtcgccag ctgacgaccg gctcccggca
    13981 gcacggctcg ccgcgctggt cgccgaacgg ggacgatatc gccttcacgt cgttccgcga
    14041 cggcaagcac ggcatctaca cgatccggcc gtccggcggc gaggctgtcc tccagaccac
    14101 gcatccgacc ccacctggcg aattggcctg ggccccgcac ggcatccagc tcgccttcgt
    14161 ggccccgtac gatcctgaat acctggacgg acctcctgac gaccccgaat tggccccgcc
    14221 ggtccgcgtc gtcaaccgga tcgactacaa gcaggagaac cgcggcttcc tcaacaacgt
    14281 ccgcaacgcg ctctggacgg tcgaaatcta caccggtgac aagcagcgcc tgaccgatga
    14341 cctgaccgac cacctcgcgc cggtttggtc gcccgacagc caactgatcg cgaccaaaat
    14401 cagccgccgc aacggcatga cctcccaact ctgcgtcacg gacgcggtcc gcggcggcac
    14461 gaaggcgatc atcgggcccg aggacggatt catctcgacc tggatcttca cgccggacag
    14521 caaggcgatc ctctactcag gcgacacgac tcagagctgg caggctgact ggtttattta
    14581 ctcgctcgag accagtgaga cgcgccgctt gactgacgac ttgcgcgtct cggtcgacgg
    14641 cggtttcccg accgtcgcct tgccggctca accggtctgg ttgaacgacg atcaggtcat
    14701 ctaccatggc ttcagccgcg gccaatcagg catttaccgg ctgaccatat cgacgggtgc
    14761 gaacgaggag ctttactcct ggccggcagt ccacgccgga ttcagcatcg acgaagagcg
    14821 cgactatatc gtccaggtcc gctcaagccc tcgcacatcg ggtgaggtga tacagatcgc
    14881 gatcgactcc ggcgagatcg atgtcatcga cgagccgaac gccgagtact tctacgcgca
    14941 ggatatcggg ccctgggagc gaatcacgat cgagcgcggc ggtctcgaga tggacggctg
    15001 gctcctgctg ccgccggagt tcgactcgtc gaagcaatac ccgctcgtgc tcgacatcca
    15061 cggtggcccg aacagccatc acgggcccgg gttcaactcg acccagcaag cgctggctgg
    15121 cgcaggattc gcggtcctct tcaccaaccc gcgcgggtca tcgagctatg gccgcgcctt
    15181 cacccaggca gtcgtcggtg attgggccgg cgaggactac ctcgaccaga tggccttcct
    15241 cgacaagatc tgctcgcgga cctacatcga tgccgaacgg gtcggcgtct acggctacag
    15301 ctacggcggc ttcatgacga gctggatcgt cggccacacc gaccggttca aggccgccgt
    15361 gatcggtgcg ccggtggtcg atctcgtctc cttctacggg acggccgaca tcggtcacac
    15421 cttcggaccg ctccaggtcg gtggcacacc gtgggactgc ccggaggagt accgcgcccg
    15481 ctcgccgctc acgaacctgc acaaggccac gactccgtcg ctgatcgtcc atggcgaggc
    15541 agacgaccgg gtgccgatcg gacaaggcga gcagtgcttt atgacgctca agcaggccgg
    15601 ctgcgtgacc gagttcgtcc gctacccggg cgaggcgcac gccatgctgc ggaccgggct
    15661 ccccgcccac cgcgaggact atcacagccg ggtcgtggcc tggttcgaga catacctgac
    15721 cgatcgcggg tcggcccagg atggatgagc agacgatcga ggcatacgag caactagccg
    15781 aggcgatctt ctcgcgccac actcgtttcg accgggcaac gcaccacgag cgcatgctcc
    15841 cctggctccg tccgggtgag ccgacagccg acgtcggcag cggctccggc gccgatctcg
    15901 cctggctcgt gtcccaaggc ttcccggccg tcggctatga gcccgtcgcg gggctgcggc
    15961 aactggcgat cgcgacgttc ccgggaattg atgtccgagc atcgtcgctg ccggatctcg
    16021 agggcgtgcc agccggtgcc ttcggcaacg tcatttgcag cgccgtgctg atgcacctac
    16081 cggtttcgtc gatctcgccc gcgatcgcca gcctctcccg gattttgcgt cccggcggcc
    16141 ggcacctgct ctcctaccgg ccaggcaagg atggcgccga acgcgaggac gatggccgcc
    16201 tcttcacccc gattgcgccg gacctgatga atcgatttct gagcgagagt gaattgcggg
    16261 tcgttcacca gagcgcaacc gctgatccaa accgccccgg cgtcatcttc gagaggttcg
    16321 tcgcggagaa actgcaacag gttccggacg tctaaccgaa tctccgaagc cgcccgggga
    16381 taaatgcccg gaataaacca cggaaagccc actaaagggg ctgcaagcga gtctgagccc
    16441 ctttagtggg cttatgttta ctcagcccga gcctttagcc tcgggcggcg ccgtagagcg
    16501 agctcatggg gtttgattcc ggttcaccgg gcggtacatg gagatcatgt cgacatagtc
    16561 gccgcggtgg tggaccgcgc ctggcaggcg ggcgtactcg gtgaagccca tcttgcggta
    16621 taggtgaatt ccgcgctcgt tgctggcgaa gacatcgagt tggatcatcc gcatcccggc
    16681 caggtatcgc tcggcctcgt cgatcacggc gtgcataagg gcggacccca cgccttgatc
    16741 gcggaagtcc gacgccagac cgatcccgaa atagccgatg tgcgttgcga ccaggctacc
    16801 cagctcgatg accgccgagc cgatataccg atcgccggca aaggcaaaga gcgggattcc
    16861 cttcccggca gccagggccg ccagccgctc gccgatccag gcctgctcct gcgccagcgt
    16921 cattcgctcg ccctggaaga gcacgaaggt gcgttcggcc gaaagcgcgt tgacgaattt
    16981 cagcgccgac ggcgcatcag tcatcagcgg ataacggaaa atgacctccc ctttggccga
    17041 ctcgtgacga ccgacgaccg tccccggtgt cggcatcgtc ggcccaggtg atgtgagtgc
    17101 ctccgcagct cgctccgcat catgcttccg ccagagcgtc gtcagccacc cgggcacggt
    17161 tgcgtccggg cgctgttcgt cccgcgggta gtagcccttg atgtcgagca ccggcgtccc
    17221 gtcccacgcg tcgagcccgg tgacatgcag atcggtacca tcgcgccgga ctagccgcgg
    17281 gcagcagacg ccgatcgggt tcggtcgccg cggagtacgc aaggcaaaga agccgactgc
    17341 cggcatctcg tcgcgaccct ccgggcgcat cggctctccg gccgtacgcc gccgttcgac
    17401 ccggtccgca tagaagagga cgacgaggtg gctgaactcc tcgagcccgt ccagacccgc
    17461 cttccagcgg ggatcgagcc ggataatcga cggcctggcg gggtccagga acgcatccgg
    17521 acctgccgca tcctcgcgaa gcaccgtccc gatcgcctgc acccgaaacc agcgtttgtg
    17581 cccggccata tcccctgccc tccgatctga gaagtatgct caacgccaga atagcggtcg
    17641 tctctgcgag gaaagggttg gcatgcacct ggcaatcggc ggcgatcacg ccggatttcc
    17701 gctcaaggga ccgatcatcg acgccctccg cgactggggt catgaggtcg tcgatctcgg
    17761 cacgcactcc accgagccgg ttgatttccc ggacatcacg aaagccgtct gcgctcgcgt
    17821 cctcaacggc gaggcggacc gggcgattct cgtctgcggg accggcgttg gcgcctgcat
    17881 cgccgccaac aagatcgacg gcatccgcgc cgctctttgc cacgatgtct actcggccca
    17941 ccaatccgtc gagcacgacg atgtcaatgt gctctgcctc ggcgcgcaga tcgtcggcct
    18001 gccgctcgcc ctcgacctgc tgagagcctt cctcgctgcc cgcttcagcg atgaggaaca
    18061 cttccgccgc cgcgtccaga agattgccga cctcgaacga gggaatctcg ccctatgacc
    18121 gctgtcgacc tgcaaaccgc gcccgatttc gccttcatga atgccgacgg caacgaggtc
    18181 cggctgtccg acttctggga atcggcgccg aaggggatcg tgctcgtctt cctgcgccat
    18241 ttcggctgcc tcttctgccg ggagcacgcc acccagcttc gcgatcacta cgccgagttc
    18301 tcaaagcgcg gctaccaggt cgtcgcggtc gggcagggaa cgcccgctcg ctccgcgaag
    18361 ttcaggaccg attacgacct gccctttccg gtcctcggcg accgcgaaat ggcgtcatac
    18421 cggctctttg gactgactag cggcggcatg ggcgggttga tccagccccg ctcctgggtg
    18481 ccgggtgttc gagccctgct ccacggcagc cggccgggca tgctggatgg cagtctcaac
    18541 caaaacccgg ggaccttcct gattgaccgg aacggccgca tccttcgcca gcaaatcggc
    18601 cagcacgccg gcgatttccc ctccgcgacc gagatcctgg gttggatcga ttccctgcat
    18661 caagcaattt ccgcgggctt ctggtaggat cagttgccag atcaagccga gattgctgtt
    18721 agtgacgcct gcgcgtccgg caacctgacc gaaacgggct gatatatggc agcgcaaacg
    18781 ctgtcccagg tgcaccaacc aaaccgattc ctcgatctct atcgccacct tgtcgacctc
    18841 gcgatcgcgt cggtcgagat ggcctttggc gattggcgcg ccctgccggt ggaccgccct
    18901 cctccatcgc ccgcccggca gtaaattccc ccgtcactgt tcgacatatc aggagtgata
    18961 ttcgtggtta gcacaccagt gcggtcgacc aaggatttga tcgatcgcga aaaagttcag
    19021 cagctcatca accgggaaga gcagcggctc aacgccaaca cggaggtttc ggcccaatac
    19081 tacgagcgcg cgaagaagca catggtcggc ggcgtgccat cgtcctacca ggcccgcgat
    19141 ccctggccga tctatctgtc g
//
LOCUS       DATAOZ010000412         5427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3851132, whole genome shotgun sequence.
ACCESSION   DATAOZ010000412 DATAOZ010000000
VERSION     DATAOZ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3851132"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VHR64_10615"
     CDS             <1..236
                     /locus_tag="VHR64_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303324.1"
                     /translation="GVQLGGLRETETIWPSVLPSDIDGSVRNAVALVQSGIQSRRTAW
                     ASLGGTDPEAEQSRVMEEARSREGAQARSEGGE"
     gene            316..930
                     /locus_tag="VHR64_10620"
     CDS             316..930
                     /locus_tag="VHR64_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303325.1"
                     /translation="MTTRLDLRLVLRRKLEDSGVNPLWDDALLNDTLWNSLVRLGVRI
                     PLEASVTVPIPPATTSIAVSPTIVRDRLLRVIDARGELVPEAISFGPSGPGEARAWRW
                     FNGTLILTRPLGTAETWTIEYRAVRSMPADDVTPVQIDPEDEPILIAMAAETVLRRYA
                     TEQMKRHGVARVPLACAEAMMAEADRLLRDRRRNVRSGVLAVLA"
     gene            1030..2967
                     /locus_tag="VHR64_10625"
     CDS             1030..2967
                     /locus_tag="VHR64_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX3303326.1"
                     /translation="MSTRLFQSMREYGRRTWAAVRTVQPDRESDTTGFESTRYLTRWL
                     VLGVLIGVVAGLGAVLFIRAIGFATEFFLGHLAGYTPPSPFGEGNLGRTGIERRWALP
                     LVTALGGLLAGLIVSFLAPEAEGHGTDAAIEAIHHKGARLRARIPPIKLVASAITIGS
                     GGSGGREGPAAQISAGFASQLAGWLKLSPRDRRIAVAAGMGAGIGAIFRAPLGGAVMA
                     AEILYLHDLEVEALIPGLIASIVGYSVFGAFEGWEPIFGAQPGIGFENPATLVYYALL
                     GIICGLIGLLYARCFYGTEAIFHKLPVPRWLSPAIGGFLVGLIGLKLDGAIHTGYGWV
                     QIGMSPELMMLPLWMVLVLPLAKVLATSLSIGSGGSGGIFGPGMVIGGLTGAAVWRLA
                     DGHLPQVPSSPAPFVIVGIIAMFGGIAHAPLAMMLMVAEMTGNLSLLAPAMIAIALST
                     ALVGDHTIYTSQLPGRADSPAHRIRMSFPLLASLSVRDAFRRAPEPASNGHAGPVLMP
                     AMPLDVALDQLAEQGVSEAPVLENGKAIGTLTVKDVITAYRSSLASGLRKSTALPSNT
                     VMIDLMLTDTSPLVGHSLAGRVLPEGTLVAAIVRQGETIVPRGDTVLLAGDHISVLTS
                     PGHAAQTRRCLRGADRSASAS"
     gene            3001..3282
                     /locus_tag="VHR64_10630"
     CDS             3001..3282
                     /locus_tag="VHR64_10630"
                     /inference="COORDINATES: protein motif:HMM:NF016148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HEX3303327.1"
                     /translation="MPIGWDPGDGGGVIDPPRAELQPPDPRDDSWERWREAYPVIWET
                     WRTSGDERVCPICGPLAGLEFRAGDGPMPPLHTNCRCTRETTRVEWVLR"
     gene            3349..3561
                     /locus_tag="VHR64_10635"
     CDS             3349..3561
                     /locus_tag="VHR64_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303328.1"
                     /translation="MGLSQIRFLSGIVVVALVLLFAFDVIGMWTFIVLIVVEAAVTAG
                     LSFRILRNQRAHAEPPATLQERIDRE"
     gene            3668..3874
                     /locus_tag="VHR64_10640"
     CDS             3668..3874
                     /locus_tag="VHR64_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303329.1"
                     /translation="MSRRRDWIAMIVTTVVLSLFVFVSTLMFGPAGFIGALLVSWSAL
                     AAIKRMHIRRTRSRRTAVGRIAVE"
     gene            3909..4256
                     /locus_tag="VHR64_10645"
     CDS             3909..4256
                     /locus_tag="VHR64_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303330.1"
                     /translation="MSYADALIDHVRSLPLPVRVIGPLVLVWLATVGFLPLLDAPFWA
                     VLLSAELGAGLYAIKDLNITGYRLLVALGILLPGSRIGGVQLPFAEMSTFVIALAYIG
                     MFILSASPARRLT"
     gene            4591..>5427
                     /locus_tag="VHR64_10650"
     CDS             4591..>5427
                     /locus_tag="VHR64_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303331.1"
                     /translation="MTQSTDVVLAGDGYMVAPGTYRRMSDGVTEGGPGRIVLRDFVGG
                     QRRAIQLETERGWDSEGVGPTLFGQGVEPWPYGTNFADPVIGVPTVTTRTVAMLLGDA
                     IYIGIGRFLYCSVALSASTWSAFTQVADLGTGTVISGLAPYQDKLAIACGATRDIQIY
                     DPVPGTLATMQTGEKGQQIVGYASRLVWSGVGSATGDLRMSTGSGIDSRPLDSPIVRM
                     ALHGGKIAIATRTSLYLLGGKADSAAGKWIGEPEPFFTHGIWTGADDYPFLISFGGKL
                     YTW"
BASE COUNT          837 a         1791 c         1844 g          955 t
ORIGIN      
        1 gcggcgtgca gctgggcggt ctgcgcgaga ccgaaaccat ctggccgagc gtcctgccga
       61 gcgacatcga cggctcagtc cggaacgcgg tcgccctcgt ccaaagcggc atccagagcc
      121 gcaggacagc ctgggcatca ctgggcggaa cggacccgga agcagaacag agtcgagtga
      181 tggaggaggc gcggagtcgc gaaggcgcgc aggcgcgaag tgagggaggc gagtagttag
      241 gctctttcca aaacccaaaa ctaactccgc gccttcgcgc ctcccgactt cgctcctcat
      301 agaaaggacc tccccatgac tacgcgactt gaccttcggc ttgtcctgcg ccggaagctt
      361 gaggatagcg gcgtcaaccc gctctgggat gacgcgctgc tcaatgacac gctttggaac
      421 agcctcgtcc ggctcggggt ccggattccg ctcgaggcat cggtcacggt gccgatcccg
      481 ccggccacga ccagcatcgc ggtgtcgccg accatcgtcc gcgatcgcct gctgcgggtg
      541 atcgatgccc gcggcgaact ggtgccggag gcgatctcgt tcgggccgag tgggcccggc
      601 gaggcgcggg cctggcgctg gttcaacggc acgctgatcc tgacccgacc gctcgggacc
      661 gccgagacct ggaccatcga gtatcgcgcg gtccgctcga tgccggccga cgacgtgacg
      721 ccggtgcaga tcgatccgga ggatgagccg atcctgatcg cgatggcggc tgagacggtc
      781 ctgcgccggt acgccaccga gcagatgaag cggcacggcg tggcgcgggt tccgctggcc
      841 tgcgccgagg cgatgatggc cgaagccgat cgcctactgc gggatcggag gcggaacgtc
      901 cgctcgggcg tgctggcggt gctggcgtga cggttgacca tgccgaggca ggaacatttg
      961 ctaggattac cgagcgatct cgtacatcgc accacgatat gcctcagttg cgggacaagc
     1021 attcaaccgg tgtcgacacg tctcttccag tcaatgcgcg aatacggccg tcgcacctgg
     1081 gcggcggtca gaaccgtcca gcccgaccgc gagagcgaca ccaccggctt cgagagcacg
     1141 cgctacctga cgcgctggct cgtgctcggg gtgctgatcg gggtcgtggc cggacttggt
     1201 gcggtcctct tcatccgcgc catcgggttt gcgactgaat tcttcctcgg ccatctcgcc
     1261 ggctacacgc ccccctcgcc ctttggcgaa ggcaatctcg gccgaacggg gattgagcgg
     1321 cgatgggccc tgccactggt gaccgcgctc ggtggactgc tcgccgggct aatcgtctcc
     1381 ttccttgcgc cggaagcgga agggcacggg acggatgcgg cgatcgaggc gatccaccac
     1441 aagggcgccc ggctgcgggc ccgcatccca ccgatcaagc tggttgcgtc cgcgatcacc
     1501 atcggctcgg gcggctccgg tggccgcgag gggccggccg cgcagatctc agccggcttc
     1561 gcatcgcaac tcgcagggtg gctcaagctg agcccgcgcg accgccggat cgcggtcgcg
     1621 gccgggatgg gcgcggggat cggcgcgatc ttccgggcac cgctcggcgg cgcggtgatg
     1681 gcggccgaga tcctctacct ccacgatctc gaggtcgagg cgctgattcc ggggttgatc
     1741 gcgtcgatcg tcggctacag cgtcttcggc gcgttcgagg ggtgggaacc gatctttggc
     1801 gcacagcccg ggatcgggtt cgagaacccg gcaacgctgg tctactacgc actgcttggg
     1861 atcatctgcg gcctgatcgg tctgctctac gcccgctgct tttacggcac ggaggcgatc
     1921 ttccacaagc tgccggtgcc gcggtggctc agcccggcga tcggcgggtt cctggtcggc
     1981 ctgatcgggc tcaagctcga cggcgcgatc cacaccgggt acggctgggt gcagatcggg
     2041 atgagcccag agctgatgat gctgccactc tggatggtgc tggtcctccc gctcgccaag
     2101 gtcctggcga cgtccctctc tatcggatcc ggtggatcgg gcgggatctt cggtcctggc
     2161 atggtgatcg gcgggttgac cggcgccgcc gtctggcggc tggcggacgg gcacctgccg
     2221 caggtcccgt catcgccggc gccattcgta atcgtgggga ttatcgccat gttcggtggc
     2281 attgcccacg ccccgctggc catgatgctg atggttgccg agatgaccgg caacctgtcg
     2341 ctgctggccc cggcgatgat cgcgatcgcg ctgtcgacgg cgttagtcgg ggatcacacg
     2401 atctacacga gtcagctccc cggtcgcgcg gacagcccgg cccatcggat ccggatgagc
     2461 ttcccgctcc tcgcctcgct gtcggtgcgg gatgccttcc gccgcgcgcc cgagccggca
     2521 tcgaacggcc atgccgggcc cgtcctgatg ccggccatgc cgttggacgt ggcgctcgac
     2581 cagctcgcgg agcagggggt gagcgaggcg ccggtgctcg agaacggcaa ggcaatcggc
     2641 accctgacgg tgaaggacgt catcacggcg taccgctcgt cgctcgcctc cgggctgcgc
     2701 aaatcgaccg cattgccgtc gaacacagtc atgatcgacc tgatgctgac cgacacctcg
     2761 ccgctggtcg gccattccct ggcgggccgg gttttgccgg agggaacact ggttgctgcg
     2821 atcgtgcggc agggcgagac gatcgtgccg cgcggcgaca cggtgctgct cgcgggcgat
     2881 cacatcagtg tcctgacgag tcctggccat gcggcgcaga cccggcgctg cctgcgcggt
     2941 gcggaccgct cagcctccgc atcctgacac gcaaatcgaa atatccgaag gaggaacctg
     3001 atgccgatcg gatgggatcc gggtgatggc ggcggcgtga tcgatccacc acgcgcggag
     3061 ctacagccgc cggacccgcg ggacgactcg tgggagcgct ggcgagaggc ctacccggtg
     3121 atctgggaga cgtggcgaac cagtggcgac gagcgcgtct gcccaatctg cggcccgctc
     3181 gccggtctcg agttccgggc cggcgacggc ccaatgccgc ccctccacac caactgccgc
     3241 tgcacccgcg aaacgacccg cgtcgagtgg gtgctgcggt gataagccgt acccaaatgt
     3301 aggggttttc cacggcaaac ccctacactg ggagcggagg tgatgggcat ggggctgtcg
     3361 caaattcggt ttctgagcgg gatcgtcgtg gtcgcgctgg ttctgctgtt cgcgttcgac
     3421 gtgatcggga tgtggacgtt catcgtcctg atcgtggttg aggcagcagt gacggccggc
     3481 ctgtcgttcc gcatcctccg aaatcagcgc gcccacgcag agccgccggc cacgctgcaa
     3541 gagcgcatcg atcgcgagtg attgtcctgc gacccgaacc acttgccaat gcgggtcgct
     3601 tccggcaatc tgaatgtgat cgctgatgcg aattcgatgc caacgctccg aacgaggaga
     3661 atttgcgatg agccggcggc gtgactggat cgcgatgatc gtaacgaccg tcgttctctc
     3721 gctcttcgtt ttcgtatcga ccctgatgtt cggcccggcc ggattcatcg gggcactctt
     3781 ggtctcctgg agtgcgttgg ccgcgatcaa gcgcatgcac atccgccgta cccgtagccg
     3841 gcgaacggcc gtcgggcgca tcgccgtcga ataagacggc gagtgtcaga tatggggaga
     3901 aagcagcgat gagctacgcc gacgcgctga tcgaccacgt ccgctccttg ccattgccag
     3961 tgcgggtgat cggcccgctg gtgctggttt ggctggcgac ggtcggtttt ctgccgttgc
     4021 tggatgcgcc gttctgggcg gtactgctga gtgcagagct gggcgcggga ctctacgcga
     4081 tcaaggatct gaacatcacg ggataccgcc tgctggtggc gctcgggatc ctgctgcccg
     4141 gttcccggat cggcggcgtc cagctgccct tcgcggagat gagcaccttc gtgattgccc
     4201 tcgcctatat cggcatgttc atcctgtcag ccagccccgc gcgtcgacta acgtagcgcg
     4261 gtccgcccct gggccgctct ggtctcgggc gtccgggtca atgagagcgc tcggctccaa
     4321 caacgccgca tcccgatgac cccatgccgg ccacgggggc cggtgctacg tctcggtaac
     4381 cagctttagc cgggttcatg tctcggcccg atgtttacgt tcgggcggcg ccgggatgcc
     4441 ggtctccatg catcgacccg gtctaccgtc agcccgggtc gcactgccat gcggcgctac
     4501 aggtcggctt cgcgcctccg cgcctgttga ctccgcgccc tcttctcgac tcctcgactt
     4561 ctcgactgct cgactcacga aaggaacccc atgacccaat ccaccgacgt cgtccttgcg
     4621 ggcgacgggt atatggttgc gcccgggacg taccggcgca tgtccgacgg cgtcacggaa
     4681 ggcgggccgg gacggatcgt cctgcgcgat ttcgtcggcg ggcagcgccg cgcgatccag
     4741 ctcgagacgg agcgcggatg ggacagcgaa ggcgtcggcc cgaccctctt cgggcaaggt
     4801 gtcgagccat ggccgtacgg gacgaacttt gccgacccgg tgatcggcgt gccgacggtg
     4861 accacgcgga ccgtcgcgat gctgctcggg gacgcgatct acatcggtat cggccgcttc
     4921 ctgtactgct cggtcgcgct ctcggccagc acctggtccg ccttcacgca ggtggccgat
     4981 ctcggtacgg gcacggtcat ctccggcctt gccccgtacc aggacaagct ggcgatcgcc
     5041 tgcggagcaa cccgcgatat ccagatctac gatccggtcc cgggcacgct tgcgacgatg
     5101 cagaccggcg agaagggcca gcagatcgtc ggctacgcga gccggctggt ctggtccggt
     5161 gtcggatcgg ccaccggcga tctgcggatg tcgaccggca gcgggatcga ctcccgtccg
     5221 ctcgactcgc caatcgtgcg gatggccctg catggcggca agatcgcgat cgcgacccgc
     5281 accagcctct atctgctcgg cggcaaggcg gactctgcag ccggcaagtg gatcggcgag
     5341 ccagagccgt tcttcacgca cgggatttgg accggcgccg acgactaccc gttcctgatc
     5401 agcttcggcg gcaagctcta cacctgg
//
LOCUS       DATAOZ010000413         8340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3585794, whole genome shotgun sequence.
ACCESSION   DATAOZ010000413 DATAOZ010000000
VERSION     DATAOZ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3585794"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VHR64_10655"
     CDS             complement(<1..723)
                     /locus_tag="VHR64_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX3303332.1"
                     /translation="MISNQASQDPDPPHEERAHRGQILIMFAFMLVALLGALGLSVDL
                     GMAFSQRRTMQSAADAGAYAGAWAVLKTRAVQTEVNTVVNQNTMNFGSITSIQCEYIN
                     DASSSLGSCTGAIPSSATGVRVTVQENHPTFFIKVVPGGPSNVTTSAVAAANIRKLGP
                     IGGGPFLPCARQAIRTDNGERMDIVDLDSNYNLIGVNKAAIGVKFNIYGPNDTVVEDC
                     GIFPDKYNGAASFDKPCLTAPGW"
     gene            complement(973..2013)
                     /locus_tag="VHR64_10660"
     CDS             complement(973..2013)
                     /locus_tag="VHR64_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX3303333.1"
                     /translation="MRPTNATRRLTGHWDTSAANNEAAAPHPGQILIMFAFMLVALLG
                     SLGLAIDLGVAFSQRRTMQAAADAGAYAGARIVSKSAGAAMTDVTAIVNANAMNMGAI
                     TTVTCQYVNDGGASLGACAGNPPGGTSGVRVTVAEQHDTFFIKVVPGGPATVTTSASA
                     RANVQRVTGFSDGPFLPCAKDTQLVNGGKMDIWDSANNKINDQAWGKQFKIHGPQVEK
                     CSIHPSDYKGLANQDLNVNLTAPGWFHFDTGNKTGLVRFDVIGANGCKAGQTPKDCVM
                     FLPIITDKGQPVPSSSTDAWVVALVPFYITEPKSNEHYGSPIKNYVIGGQDGTGGWDP
                     TWSGAITIRMTE"
     gene            complement(2332..4434)
                     /locus_tag="VHR64_10665"
     CDS             complement(2332..4434)
                     /locus_tag="VHR64_10665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX3303334.1"
                     /translation="MEAARPAGETIVRAKPPTVERAFRPDIEGLRAFAVLLVLFNHAG
                     VPFLRGGYIGVDVFFVLSGFLITGLLVRELEQTGSISLLNFYARRARRLLPAGTLVLV
                     ATVIASYRYLGASRADRVAEDARWAALFASNIRFIRTGTDYLGSQLPPSPLQHFWSLA
                     VEEQFYAVWPALILLLSLGPKRLPIRFRLGVALLAIIGASLLWSIHQTRVDGTTAYFS
                     PLTRASELGAGALLAVAVPWLIRVPRRIAPVASWMGLAGVLLAALSYSSTTAFPGFAI
                     VLPVIGAVLVVLAGTVEPGSGAERVLRQGLFQWLGKLSYSLYLWHWPVLVIGAGRAGR
                     DLTVAENLVLCVIALGLSAATYFLIEDPVRNAKILKRRAPAVSVAIGAAMVALAFGFA
                     GWKLSGGADQSGTVIAASLPSNYATPQDVARAVAQGAGVTKWPDQPKRIANPAYSKEC
                     DVTRKDTTSSVCVHGNPNGSRTLVVYGDSHAAMWIPALDVIGREAGWQVIQLTKPACQ
                     APDFPRYSGTLKREYTECAEYREFALAQIASIKPDLVLISSAFQDAEVWRDGKPTTDG
                     LEEAWADGLTSVIQRITPNAGRIIVIGDMAYPAEPGIDCLTAHDGHVPPCNTSRRDAV
                     YAEHNAREQSVAEANGAGYVDTIPWFCTNTLCPAVIAGLTTHRDAYHVAENYVVWLAG
                     VLGEAIGILPPRNSPLHA"
     gene            4676..5731
                     /locus_tag="VHR64_10670"
     CDS             4676..5731
                     /locus_tag="VHR64_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303335.1"
                     /translation="MTATRLSISPNGTTHPAEAREIGLCFSCDEDPGIRRRKRGRGFQ
                     YLDDDGATVMDPDVIERITALAIPPAWTDVWICRKPNGHLQATGRDDRGRKQYRYHPK
                     WRAHREETKYERMAAFGRILPTIRQRVEQDLHRQGLPREKVLAAVIALLERTRIRVGN
                     DEYARANKSFGLTTLLDRHVSAGSTSVRFRFRGKSGKDHEVTLQDRRLARIVKRCQDI
                     PGQRLFQYEDDGGERHRVGSADVNDYLREIAGDDYTAKDFRTWAGTVIATGVLRHLPK
                     PEAEAAATRDILAAVDAVAEELGNTRAVARGSYIHPGIFVAYRAGQLQRIDPDDVDDP
                     EPGGLDDDERVVLAVIA"
     gene            complement(5744..7225)
                     /locus_tag="VHR64_10675"
     CDS             complement(5744..7225)
                     /locus_tag="VHR64_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3303336.1"
                     /translation="MSTLRALPSVPTDAIAETNPSVRFRRRTLLGLAAGGSAALAFSH
                     SLAPSLPSARAQTADGSVLSWRTWILKSADELRPEAPGDPSQSEMDEILSLQSKRSYG
                     NVSQITQWGTYSAVIPWAEVGIATAGEAKLSGIRDMRVQAILRAAMYDAVLAAYDAQA
                     AHPRRAPALADSRVTPHASTATGAKLSFPSVHATVAGAAFTVLAALFPDAPAGFFADM
                     ALEAAESRIMAGESYRSDVVAGLELGRAVGSRAVDYANADGSDAKWDGTGQPTGDGYW
                     QPTPPANVKDPVEPLANTWKLWFMPSADALRPAIPPAYGSLGWQAELDAVRRIVSART
                     IEQERTIDRWLQAGPHGMLLRQANELMTASGTDEAHAAQVVGLMGLATMDAVIGVWDA
                     KYKWWTARPITEDPTINTYFQTPPYPSYPSGFSAVVGASASVLAQTFPEASIEMLAMA
                     TEGAAQRCWAGIHYILDDDVGLQMGCCAGRMAFEKGLQDTAGA"
     gene            complement(7476..>8340)
                     /locus_tag="VHR64_10680"
     CDS             complement(7476..>8340)
                     /locus_tag="VHR64_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX3303337.1"
                     /translation="KNKGNHEDNGKHKGKDKDKKEKKSKKVKAEPLAAYVVHAECSYE
                     PTSDQTTCDFDADSPAGAKKINLLDLPSDEICAPVVGGDARYVDPDPNTGATGYRSTD
                     PEGKFTLVFSGRVTAGGTATYWIKAAKDIFPAKGMGLRCASQQEAAAPTPATTATVVT
                     SAPTATATPIVPEVTDTTGSVVVRAYDCPIASPPADYDWYGQCTPVTAGSRYRLMRLD
                     SATRDGITTSTDSAGRAIFRSLPPGTYQLTQTDGDWCHAQSDDVNADGNLIVKAGDRT
                     SVWVFTCASTP"
BASE COUNT         1504 a         2558 c         2747 g         1531 t
ORIGIN      
        1 ccatcctggc gcagtcagac acggtttgtc gaagctggct gctccgttgt acttgtcagg
       61 gaagatgccg cagtcctcga cgaccgtgtc attcgggccg tagatgttga acttgacgcc
      121 gatcgcagct ttgttgaccc caatcaggtt gtaattgctg tcgagatcca caatgtccat
      181 gcgttcgccg ttatccgtcc gaatcgcttg ccgcgcgcac ggcaagaatg gtccacctcc
      241 gatcggcccg agcttgcgaa tgttggcggc ggcgactgcg gaggtcgtga cgttgctcgg
      301 gccgccgggt acaaccttga tgaagaatgt cgggtgattc tcctggacgg tcacgcggac
      361 gcccgtcgcg ctggacggaa tagcacccgt gcagctaccg agcgaactac tagcgtcgtt
      421 gatgtattcg cactggatcg acgtgatcga gccgaagttc atcgtgttct gattgacgac
      481 ggtattgact tccgtctgga ccgccctcgt tttcagtacc gcccaggccc cggcgtaggc
      541 gccggcatca gcggccgatt gcatggtgcg gcgctgggag aaggccatac cgagatcgac
      601 cgacaggcca agcgcgccca gcaaggcgac aagcatgaag gcgaacatga tcaggatctg
      661 cccgcgatgg gcgcgttcct cgtgcggtgg gtccgggtct tgcgatgctt ggttggagat
      721 catagggatc gggctcctcg tatgagcggg tcccccggat gggtcccgat cggtagcacg
      781 atcgtactga cggtattgcc ggaagtacca tgaccgccga tagcccgatg gggggagccc
      841 ggaaaagtac cgcggtacta tcgcccgttt cgaccttttg gcgagaggac aaataatgaa
      901 atcgacccgg cggttatccg ccgggtcgat taattcgtgc ctgttggacg aggacgactt
      961 gccgccgacg agctactcgg tcatgcggat cgtgattgcg ccgctccagg tcgggtccca
     1021 acccccggta ccgtcctggc cgccgatcac gtagttcttg atcggagagc cgtagtgttc
     1081 attggacttc ggctcggtga tgtagaaggg aaccagggca acgacccacg catcagtcga
     1141 agacgacggt accggctggc ccttgtcggt gatgatcggc aggaacatca cgcagtcctt
     1201 cggagtctgc cccgccttgc agccattcgc gccaatgacg tcgaaccgta ccaagcctgt
     1261 cttgttgccg gtatcgaagt ggaaccaacc cggcgcagtc aggttgacgt tcaggtcctg
     1321 gttggcaaga cccttgtagt cactcggatg gatcgaacac ttctcgacct gcgggccgtg
     1381 aatcttgaat tgcttgcccc aggcctggtc gttgatcttg ttgttcgccg aatcccaaat
     1441 gtccatcttg ccgccattca ccagctgggt gtccttggcg cagggtagga acggaccgtc
     1501 agagaacccg gtcacgcgtt ggacattggc ccgggcgctg gcggaggtcg tgacggtcgc
     1561 gggtccgccc ggcacgacct tgatgaagaa cgtgtcgtgc tgttcggcga cggtgactcg
     1621 cacgccgctg gttccgccgg gcggattgcc ggcacaagcg ccgagcgacg ctccgccgtc
     1681 attgacgtac tggcaggtga ccgtcgtgat cgcgcccatg ttcatcgcgt tggcgttgac
     1741 gatcgccgtt acgtcggtca tggccgcgcc agcagacttc gaaacgattc tcgcgccggc
     1801 ataggcgccg gcatctgccg ccgcctgcat cgtgcggcgt tgcgagaaag cgacgccgag
     1861 atcgatcgcg aggccgaggg agcccagaag cgccacgagc ataaatgcaa acatgatcag
     1921 gatttgtccg ggatgcgggg cagcagcctc attatttgcc gcggacgtgt cccagtgtcc
     1981 ggtcaagcgc cgggtcgcat tagtagggcg catttgcgtt gcctccgttc tgcataccga
     2041 tccagcgttt tcattctgcc gatccggctt ccacgcgcga gttcaaccca cgctctgact
     2101 cctccagttt tcgctcccga acgtcccatc gcctaacgtg cgcgagagcc ggaaacaggc
     2161 ggcgactgtt gacccgctcg gtcgtcggcc gcgcgtacct acaagccgaa tggcgcaagg
     2221 ccatccgcct atgagattta tgatgacgaa tgcggaacgc cacggatacg cgtacgtacg
     2281 cactgcgggg ctaaatgacg acggtacttc gtaccgtccg gacagggacc tttaggcgtg
     2341 tagcggtgag tttctcggag gcaggatgcc gattgcctcg ccgagcacac cggcgagcca
     2401 aacgacgtag ttctccgcca cgtggtaggc atcgcggtgc gtggttagcc cggcgattac
     2461 tgccgggcag agcgtgttgg tgcagaacca gggaatcgtg tcgacgtaac cagcgccgtt
     2521 cgcctccgct acggactgct cccgtgcgtt gtgctcggca tagaccgcgt cccgccgaga
     2581 cgtgttgcag ggtggcacat gcccgtcgtg cgcggtcagg caatcgatgc caggctccgc
     2641 gggataggcc atgtcgccga tgacgatgat ccggcccgcg ttcggggtga tgcgctggat
     2701 gaccgacgtc aatccgtcgg cccatgcttc ttcgagaccg tcggtggtcg gtttgccatc
     2761 ccgccagacc tcggcatcct ggaatgcgct cgagatcagg accagatcgg gtttgatgct
     2821 ggcaatctgc gcgagggcga actcccggta ctcggcacat tcggtgtatt cgcgcttgag
     2881 cgtgccggaa tagcgcggaa agtccggtgc ctggcaggcg ggtttggtga gctggatgac
     2941 ctgccaccct gcctcccggc cgatcacgtc cagcgccggg atccacatgg cggcgtgcga
     3001 gtcgccgtag acgacgagcg tgcgcgaccc gttcggattg ccgtggacgc agaccgacga
     3061 ggtcgtgtcc ttgcgggtga cgtcgcattc cttgctgtag gcgggattgg caatccgctt
     3121 cggttggtcc ggccacttgg tgaccccggc cccttgagca accgcccgcg cgacgtcctg
     3181 cggcgtcgca tagttcgagg gaaggctggc ggcaatgacc gttccggatt gatcagcgcc
     3241 gccgctcaat ttccacccgg caaaaccgaa tgccaaggcg accatcgcgg ctccgatcgc
     3301 gacgctcact gccggagctc gccgtttcag gatcttcgca ttccggactg ggtcttcgat
     3361 gaggaagtag gtggctgccg aaagccccag ggcaatgacg cagagaacga gattctcggc
     3421 aacggtcagg tcgcgaccgg cccggccggc gccgatcacg agcaccggcc aatgccaaag
     3481 gtaaagcgag tacgacagct tcccgagcca ctggaatagc ccttgccgga gcacgcgctc
     3541 ggcgcccgaa cccggttcga ctgtgccggc cagcacgacc agtaccgcac cgatcaccgg
     3601 cagcacgatc gcaaaacccg ggaaggctgt ggtgctggag taggagagcg ccgcgaggag
     3661 gacgccggcg agtcccatcc agcttgccac cggcgcgatc cggcgcggga cgcggataag
     3721 ccacggtacg gcaacggcca gcagcgcacc ggcgccgagc tcggaggccc gggtcagcgg
     3781 cgagaagtag gcggtcgtgc catcgacccg ggtttggtga atagaccaga ggagcgatgc
     3841 cccgatgatc gcgagcagag cgacaccgag ccggaatctg atcggcaggc gcttcgggcc
     3901 aagcgagagg agcaggatca gcgccggcca gaccgcgtag aactgctcct cgaccgcgag
     3961 cgaccagaag tgctggagcg gtgagggtgg gagctgagaa ccgaggtaat cagttccggt
     4021 gcggatgaag cggatgttac ttgcaaagag cgccgcccag cgcgcgtcct cggcgaccct
     4081 atccgcccgg ctcgcgccga gatagcgata cgacgcgatg acggtcgcga ccaggacgag
     4141 tgttccggcc gggagcaacc gccgggcgcg acgggcgtag aagttcagga gcgagatcga
     4201 cccggtctgc tcgagctcgc gaaccagcaa tcccgtgatc aggaagccgg aaagcacgaa
     4261 gaagacatct acgccgatgt acccgccgcg aaggaacggc acgccggcat ggttgaagag
     4321 cactagcagg acggcgaaag cacggagtcc ctcgatatcg ggccgaaagg cgcgttcgac
     4381 ggtcggtggt ttggcgcgaa cgatggtttc tcctgccggt cgcgcagcct ccaaatcgaa
     4441 tctccggatg tgtgggcgta gtgccgagag caggaacgat cctgacagtt gcgtcgttgc
     4501 aaccggagca tcctgccacc catctcaccc aacccggtgt gtttgcgcaa caccgcatcc
     4561 aattcgtcat ggcgtcgagg agggagtcag ggatccgtgt atcgcggcga atttccggat
     4621 tgattgcgct caatcacaat gggctcagtg ttcatttgac tggggaaacg gaacgatgac
     4681 cgcaacaaga ttgtcgattt cgccgaacgg cacaacccat ccggccgagg cgcgcgaaat
     4741 cgggctttgc ttctcgtgcg acgaggatcc gggcattcgc cggcgcaagc gcggccgtgg
     4801 atttcagtac ctcgatgacg acggggcgac cgtcatggac ccggacgtca tcgagcggat
     4861 cacggctctg gcgattcctc cggcctggac cgatgtctgg atctgcagga agccaaacgg
     4921 gcacctgcag gccaccggcc gggacgatcg cggacgcaag caataccggt atcacccgaa
     4981 atggcgggcg caccgggagg agaccaagta cgagcgcatg gccgccttcg gccgcatcct
     5041 gccgaccatc cggcagcgcg tcgagcagga cctgcatcgc cagggattgc cgcgcgagaa
     5101 ggtgctggcg gcggtgatcg cactcctgga acgaacccgg atccgggtcg ggaacgacga
     5161 gtacgcgcgc gccaacaagt cgtttgggtt gaccacgctg ctggaccggc acgtctcggc
     5221 tggctcaacc tcagtccggt tcagatttcg aggcaagtcc ggcaaggacc acgaagtgac
     5281 gctccaggat cgccgcctgg ccaggatcgt caagcgctgc caggacattc ccggtcagcg
     5341 cttgttccag tacgaagatg acggcggaga gcggcatcgt gtcggatcgg ccgacgtcaa
     5401 tgactacctc cgggagattg ctggcgacga ctacacggcc aaggatttcc ggacctgggc
     5461 cgggacggtc atcgcaaccg gcgtgctcag gcatctcccc aaacccgaag cagaggccgc
     5521 cgccacccgc gatatccttg ccgccgtcga cgcggtcgct gaagagttgg gcaacacccg
     5581 agcggtcgcg cgtgggtcgt atatccatcc gggcatcttc gttgcctacc gggctggcca
     5641 gctgcagcga attgatccgg acgacgtcga cgacccggag cccggcgggc tggatgacga
     5701 cgagcgggtc gtgctcgccg tcatcgcctg atcgcacgag cgcttacgct ccggcggtgt
     5761 cctgcagacc cttctcaaat gccatgcgcc cggcgcagca acccatctgc agcccgacat
     5821 catcatccag gatgtagtga attccagccc agcaacgttg tgccgcgcct tcagtggcca
     5881 ttgcgagcat ttcgatcgac gcctccggga aggtttgtgc cagcacactt gccgaggcgc
     5941 cgaccactgc cgagaagccg gaggggtaag atggatatgg tggtgtctgg aagtaggtat
     6001 tgatggttgg atcctcggtg attggccggg cagtccacca cttgtatttg gcatcccaga
     6061 ccccgatcac ggcatccatc gtcgcgagcc ccatgagacc gacgacctgg gcagcgtgcg
     6121 cctcgtcagt cccggaggcg gtcattagct cgttggcttg cctcaggagc ataccgtgtg
     6181 gccctgcctg caaccatcga tcaatggtcc gctcctgctc aatcgtgcgg gcgctgacga
     6241 tgcggcgaac ggcgtcgagt tctgcctgcc agccgagcga gccgtaggca ggcggtatgg
     6301 ccggacgaag ggcatcggca ctgggcatga accacagctt ccacgtgttg gcgaggggct
     6361 cgactggatc cttgacgttg gctggtggcg ttggttgcca gtaaccgtca ccggtcggct
     6421 gaccagttcc atcccacttg gcatcggagc catcggcgtt ggcgtaatcg accgctcggg
     6481 agccgaccgc gcgaccgagc tcgagccccg cgacaacatc gctgcgatag ctctcgccgg
     6541 ccatgatgcg cgattcagcc gcctccaggg ccatatcggc gaagaagcct gccggcgcgt
     6601 ccgggaagag ggcagcgagc acggtgaatg ccgcgccggc cacggtcgcg tggaccgagg
     6661 ggaacgacag cttggctccg gtggcagtcg acgcgtgggg cgtgactctg ctgtcggcca
     6721 atgcgggagc gcgacgggga tgggctgcct gggcgtcgta ggcggcgagc accgcgtcgt
     6781 acatcgccgc ccggaggatg gcctgaacgc gcatgtcgcg gatgccggac agcttggctt
     6841 ctccggcagt ggcgatgccc acctctgccc acgggatcac tgccgaatat gtcccccatt
     6901 gggtgatctg actgacgttg ccatacgagc gcttcgattg cagggacagg atttcatcca
     6961 tctcggactg tgacggatcg cctggcgctt cggggcgcag ctcgtccgcc gacttgagga
     7021 tccacgttcg ccagctcaaa acgctgccat cggccgtctg tgctctggcg gagggcaggc
     7081 tgggagccag cgagtggctg aatgccagcg cggcgctgcc acccgccgcc aaccccagga
     7141 gggtgcgtcg ccgaaatcgt acagacgggt ttgtctctgc gatggcgtcg gtgggaacag
     7201 acgggagggc gcgcagcgtc gacatgatcc ttaccctttc ctgggggatg acagccagcg
     7261 aagctccgtg ccaaggcgaa ggttcgagcc tgcgccgacc acagtggctg accgtctgag
     7321 cgaaatgaag gagatgtgtg ctgtccctct acgataaggc agttgtcaat acttaccatt
     7381 agcacgagaa atgacgtggc gccgagcacg caaacgtgcc tggcagcgcc agctttcggc
     7441 gcgctgccag gctcggattg gccgttaccg atcagttacg gagtgcttgc gcaggtgaag
     7501 acccagaccg atgttcggtc gccggccttg acgatcagat tgccatcggc gttgacgtcg
     7561 tccgactgtg catggcacca gtcgccgtct gtctgcgtga gctggtaggt gcccgggggc
     7621 agcgagcgga agatggcacg gcccgcgcta tcagtcgagg tcgtgattcc gtcgcgcgtc
     7681 gcgctgtcga ggcgcatcag gcgataccgc gatccagcgg taaccggggt gcattgcccg
     7741 taccagtcgt agtcggccgg aggcgaggca atcgggcagt catacgctcg gacgacgact
     7801 gacccggtcg tgtcggtcac ttccggaacg atcggcgttg cggtggcagt gggcgcggaa
     7861 gtgactacgg tcgccgtcgt cgcgggcgtt ggcgctgccg cctcttgctg acttgcgcac
     7921 cgcaggccca tccccttggc cgggaaaatg tccttggccg ccttgatcca gtaggtggcg
     7981 gtgccaccgg ccgtcacgcg gcccgagaac acgagcgtga acttgccttc gggatcggtc
     8041 gaccgatatc cggtcgcacc ggtgttcggg tccggatcga cgtatcgcgc gtcccctccg
     8101 acgaccgggg cgcagatctc gtcggacggc agatcaagga ggttgatctt cttcgcgccg
     8161 gccgggctgt cggcatcgaa atcgcatgtc gtctggtccg atgtgggctc atagctacat
     8221 tcggcgtgga cgacgtaggc agcgagcggt tccgccttga ccttcttgga tttcttttcc
     8281 ttcttgtcct tgtcttttcc cttgtgcttc ccgttgtctt cgtggttgcc cttgttcttg
//
LOCUS       DATAOZ010000414         2547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5432296, whole genome shotgun sequence.
ACCESSION   DATAOZ010000414 DATAOZ010000000
VERSION     DATAOZ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5432296"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1201)
                     /locus_tag="VHR64_10685"
     CDS             complement(227..1201)
                     /locus_tag="VHR64_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX3303338.1"
                     /translation="MPLKPTLLMRPLLASAVICLAPAIVHVQEKKPQPGTKWTDEQLK
                     QAVAPMRAGRKLTPKSWPNNAKVAVCLSWDMDNESFDLAAGITAPVVLSQGEYGTTEG
                     LPRIMELYDRHNIPGSFYIPAVTGLLYPEVVSELKKRPRHEVGIHGWIHESLPDIDDR
                     AEEERLLNKAIDFWTKAMGKKPVGYRAPAWAFSKYTLDLIRKAGFQYDSSAMGMDEPY
                     ELMSNGQATGMVELPVDWILDDYPYFISSGALPSPELIFKVYQDEFDRAYKDGTMFML
                     TMHPMISGHRSRIEYLDKLITYMKSKPGVWFATGQQIADYVRPQQKTQ"
     gene            complement(1227..>2547)
                     /locus_tag="VHR64_10690"
     CDS             complement(1227..>2547)
                     /locus_tag="VHR64_10690"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX3303339.1"
                     /translation="GRWLPLEAQADRIRVKGGADVNVEHLYTRHGPVVAVDTNRHQAV
                     VLRWTGDEPGTVGYLGNLSLDRAASAREFRAALVHHKMPAENFVYADVDGNIGYQAAG
                     LSPIRKGWPGLLPVPGWTGEHEWEGWFTLDDLPHAENPASGWLATANNNVLPPGEKKP
                     IGYEWLDPARINRIREVLGTADKFDIKAFERLQHDAVAWNAEQLVPLLDHVRIEETDG
                     DRARRLLLGWDRTMARTSVAATIYATWEDQLVATLIADKITGPLAAEFVARGGDWLVP
                     ALTHPNATWFGQAGARGRDSALVTALTQAVAGLKQKLGPDLDQWKWERLHTATFRHPL
                     GVNPSLASLFNVGPIARDGYALTPLSTGGPSYTQTIGATFREIMDLSDWDRSVATSAP
                     GQSGQPGSPHFSDLAKLWQDGQYFPLPFTETAVRAASETTLTLTPSK"
BASE COUNT          452 a          808 c          771 g          516 t
ORIGIN      
        1 ggcacaccaa gaatccaggc taccaagtag cgcatgtcgc tctccctgcc cccggtcggg
       61 cgtcggtgag tggagcaaga agaacgccac tgttctgctc agccggtcac gtcggtacgg
      121 cggtaaccgg cggtcgtaaa ggtcgtctac ttcctgctca aaaaggcgcg cgatcagtcg
      181 gcgcgtccac aatccgcgtc caacgatcgt tcatgcgtcg gggtggtcac tgcgtcttct
      241 gctgcggtcg cacgtagtca gcgatctgct gtcccgtcgc gaaccagacg ccgggcttcg
      301 atttcatgta agtaatcagt ttgtcgaggt actcgatccg agaacgatgg ccgctgatca
      361 tcgggtgcat cgtcagcatg aacatcgtgc cgtccttgta cgcccggtca aactcgtctt
      421 ggtacacttt gaagattagc tcgggcgatg ggagcgcgcc gctcgagatg aaatagggat
      481 agtcgtccag gatccaatcg acaggcaatt cgaccatgcc cgttgcctgg ccgttcgaca
      541 tcagctcata aggttcgtcc atccccatcg cgctgctgtc gtactgaaac cctgctttgc
      601 gaatcagatc gagtgtgtac ttgctgaacg cccaggcggg agcccgatag cccaccggtt
      661 tcttccccat cgccttggtc cagaaatcaa tcgccttatt gagcagccgc tcttcttccg
      721 cgcgatcgtc gatgtccggc aagctctcgt gaatccatcc gtggatgccc acttcgtgcc
      781 ggggtcgctt cttcagttct gacacaactt ccggatacag caagcctgtg accgccggaa
      841 tgtagaacga accggggatg ttatgccgat cgtacagctc catgatccgg ggcagccctt
      901 ccgtcgtgcc gtactcgccc tgcgacaaga ccacgggtgc cgttatgccg gccgccagat
      961 caaacgattc gttgtccatg tcccagctga ggcagacggc gaccttggca ttgttcggcc
     1021 agctcttggg cgtgagcttg cggccggcgc gcatcggcgc gaccgcctgc ttgagctgtt
     1081 cgtcggtcca tttcgtaccg ggctgaggct ttttttcctg gacatgcacg atggcgggcg
     1141 ccaggcagat caccgcggac gcgagcaatg gccgcatcaa cagcgtcggt ttcagtggca
     1201 tcgttcctcc tctgttcgac atgacgtcac ttcgatggcg tcagcgtaag tgtggtctcg
     1261 gacgccgctc gaaccgcggt ctccgtgaaa ggcagcggaa aatactgtcc gtcttgccag
     1321 agcttcgcca aatcagaaaa atgcgggctc cccggctggc ccgattggcc cggcgcgctg
     1381 gtcgcgaccg accgatccca gtcggagagg tccatgatct cgcggaacgt ggcgccgatc
     1441 gtctgggtgt acgatggtcc gccggtcgag agcggcgtaa gcgcataccc gtcgcgggcg
     1501 atcggtccga cattgaatag cgacgccaga gagggattca caccgagcgg gtgacggaac
     1561 gtcgccgtgt gcagccgctc ccatttccac tgatccaagt cgggtccgag tttctgtttg
     1621 agcccggcga cagcctgtgt gagcgccgtt accaacgcac tgtcgcggcc gcgcgctccg
     1681 gcctggccaa accacgtcgc attcgggtgg gtgagcgcgg gcaccagcca gtcgcctcca
     1741 cgcgcgacga attcggccgc cagcggtcca gtgatcttgt cggcaatcag tgtcgctacc
     1801 aattgatctt cccacgtcgc atagatagtg gctgcgacac tggtgcgcgc catcgtgcgg
     1861 tcccaaccga ggagaaggcg ccgcgctcgg tcaccgtcgg tctcctcgat ccgcacatgg
     1921 tcgagaagcg gcacaagttg ttctgcattc cacgcgaccg catcatgctg caaccgctcg
     1981 aacgccttga tgtcgaactt gtccgcagtg ccgagcactt cgcgaatgcg attgatgcgc
     2041 gcggggtcaa gccactcgta cccgatgggt tttttctcac ctggtgggag cacgttgttg
     2101 ttcgccgtgg caagccaacc gctcgccggg ttctcggcat ggggcaggtc gtcgagcgta
     2161 aaccagccct cccactcgtg ttcgccggtc cagccaggca caggcagcag accgggccac
     2221 ccctttcgga tcggggagag gcccgcggcc tggtagccga tgttgccgtc cacgtcggca
     2281 tatacgaagt tctcggctgg catcttgtgg tgcacgagcg cggcccggaa ctcgcgcgcg
     2341 ctggcggcgc gatcaagcga cagattgccc aggtagccca cagtaccagg ttcgtcgccc
     2401 gtccagcgca acacgacagc ctgatgccga ttcgtatcga ccgcgaccac gggaccgtgg
     2461 cgggtgtata ggtgttcgac gttaacgtcg gcgccccctt tgacgcgaat gcgatccgcc
     2521 tgcgcttcga gcggcagcca tcgacct
//
LOCUS       DATAOZ010000415         5131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9375510, whole genome shotgun sequence.
ACCESSION   DATAOZ010000415 DATAOZ010000000
VERSION     DATAOZ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9375510"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VHR64_10695"
     CDS             complement(<1..186)
                     /locus_tag="VHR64_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303340.1"
                     /translation="MMTVEERVARLAAKVAQELPSVGVIGLGSGTTAEAVVRALGERI
                     AGEPGFSAIGVATSTRTA"
     gene            complement(183..953)
                     /gene="pgl"
                     /locus_tag="VHR64_10700"
     CDS             complement(183..953)
                     /gene="pgl"
                     /locus_tag="VHR64_10700"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX3303341.1"
                     /translation="MTDGTIDYGERGRVVVLPDARSLAQHAAELLRDLTREVVADRAN
                     AYIALSGGSTPKAMGAILGAQPMRDEVPWGSLHVFWGDERWAPIDSPDSNAGEALRGY
                     LDSVPIPRSQVHPFETSGDPAESAANYERLIKTVVPGSPMPMFDLILLGMGDDGHTAS
                     LFPGTAALSITDRLVTENRVEKLNATRLTFTAPLINAARHVAFLVTGAGKAERLAQVL
                     DGPQDPMQLPSQLVRPTHGQLTWLVDKAAAANLAREPR"
     gene            complement(950..2149)
                     /locus_tag="VHR64_10705"
     CDS             complement(950..2149)
                     /locus_tag="VHR64_10705"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX3303342.1"
                     /translation="MTDLLQPVAITRNEHWDADQINPDDVRKVLNRMWGEIGAERRSG
                     NRLARAVADAASMRTQTVNLVVITDGNVDQPDVAQLVTHLPDISPSRVVILSSQHGWA
                     QNLKVGVDIEERPNKPPHAPTRIEVISVRGRGERLASVATPLLVPELPDFVWCTTPEF
                     AQDPVLDELSEQVDRITVDSAASPNPATALNYLVHLVKAGGSELKISDMAWTRLRSWR
                     QMVAQFFDNPSHLSCLSETEEVIIECARRDGTGKSGITGGLLTAAWLAYCMEWRAPGE
                     ELVRSRDGWKLTLRAGQKGQSREVVILIKEVDDDASAGGLSSIKLTSGITAAGTFLVR
                     RTGPDTMTTVSDTPHAGISERTIYSANPDQADLLSRELREFDSDPVFEQSLQIAANMW
                     PAGAAVA"
     gene            complement(2161..3786)
                     /gene="zwf"
                     /locus_tag="VHR64_10710"
     CDS             complement(2161..3786)
                     /gene="zwf"
                     /locus_tag="VHR64_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX3303343.1"
                     /translation="MSDSPVITAGGGTLTAERVRHKPASKIVNPLRIGLQTERNPQAC
                     AMVIFGVSGDLTSRKLMPALYDLYVSVPLPPGFSVVGISRRDWSDDDFRCEMRDAVRE
                     NATRPFTEDAWDSFARGLFYVRGDFDNSELYGRLRERLETVDSERGTNGNHLYYLATP
                     PSFYEEISAGLGNAGLVKRQEIYSNPRANGWDRIVIEKPFGHDLASAQHLNAAINAVF
                     AEQQIYRIDHYLGKETVQNVLAFRFANILFEPVWNRHYVDQIQVTVAESLGIENRATY
                     YEEAGALRDMVQSHMLQLLSVVAMEPPAFFNGDALRDEKVKVLRSVVPPDGDRVAKDV
                     VRGQYADGYVAGTEVVGYREEEGVDSDSRTETFVALKLEIDNWRWNGVPFYLRTGKRL
                     PRRVTEIAIQFKSVPHLMFKLTEGQTLPPNVLTMRIQPDEGISLRVAAKVPGAGTKLQ
                     PVQMDFDYGASFGQAGPDAYVRLLLDAMLGDPTLFARDDEIDTAWSLMQPILDVWADQ
                     PSTMPIPTYEAGTWGPAEAELLMANDRRTWRRP"
     gene            complement(3850..>5131)
                     /gene="gndA"
                     /locus_tag="VHR64_10715"
     CDS             complement(3850..>5131)
                     /gene="gndA"
                     /locus_tag="VHR64_10715"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX3303344.1"
                     /translation="FKALPELLAVLRRPRRLIMLVKAGEVVDQTIAALRPLLSEGDMI
                     VDGGNSFFQDTERRAKESESKPFDFVGMGVSGGEEGALWGPSLMPGGPAKAYEILEPM
                     LTAIAAKTEFGACVTHCGPGGAGHYVKMVHNGIEYGDMQLIAETYDIMKRALGMSTSE
                     MADVFGRWNQGKLSSFLIEITAKVLGTKDAVTGDPLVEKILDAAEQKGTGRWTSQNAL
                     DLGTPIPVIDAAVFARSISSFKARRVGASKILTGPGTAMEIADAEKQRLIGVLEDALY
                     VAKISSYAQGMALLRAASDTYSYGLNLSELARIWTGGCIIRAKLLEPIMTAYRNNPAL
                     DNLLLAPEFVDTVNSASNACREAVVLARMHGLPCPAMSAALDYIDSYRTERLPANLIQ
                     AQRDYFGAHTYKRIDTTGVFHANWDTGEAEQIQG"
BASE COUNT          857 a         1631 c         1703 g          940 t
ORIGIN      
        1 ggcagtccgg gtggatgtgg cgacgccgat cgcgctgaaa cccggctcgc ctgcaatccg
       61 ctcgccgagc gcccgcacga cggcttcggc cgtggtgccg gagccgaggc cgatcacgcc
      121 gacgctcggc aactcctgcg ccaccttcgc cgccaacctg gcgacgcgct cttcgaccgt
      181 catcatcgag gttcacgggc cagattagct gccgcggcct tgtcgaccag ccaggtcaac
      241 tgaccatgcg tcggccggac cagttgcgac ggaagctgca tgggatcttg cgggccgtcg
      301 agcacttggg caagacgctc agccttccca gcaccagtga ccagaaaggc gacatggcgc
      361 gcggcgttga ttagcggcgc ggtgaaggtc aggcgagtgg cgttcaactt ctcgacccgg
      421 ttctcggtga cgagtcgatc ggtaattgag agcgccgccg tgccgggaaa gagcgatgcg
      481 gtatggccgt cgtcgcccat gccgagcagg atcagatcga acatcggcat ggggctgcca
      541 ggcacgactg ttttgatcag ccgctcatag ttcgctgctg actctgccgg atcgcctgac
      601 gtctcgaagg ggtggacctg actgcgggga attggtacgg aatccaggta cccgcggagc
      661 gcctcgccgg cattgctgtc cgggctgtcg attggtgccc agcgctcgtc gccccagaag
      721 acgtggagcg atccccaggg aacctcgtcg cgcatcggct gcgcaccgag gatcgcaccc
      781 attgctttcg gtgttgagcc gccggaaagc gcgatatacg cgttcgctcg gtcggccacg
      841 acctctcgcg ttaggtcccg gagcaattca gcggcgtgct gggcaagcga ccgggcatcc
      901 gggagaacaa cgaccctgcc gcgctcgccg tagtcgatcg tgccgtctgt catgcgaccg
      961 ccgctccagc cggccacatg ttcgccgcga tctgcagtga ctgctcgaaa accggatcgg
     1021 aatcgaactc gcgcagctcc cggctaagga gatcggcttg gtcggggttg gcagaataaa
     1081 tcgtgcgctc ggagatgccc gcatggggcg tgtccgagac ggtggtcatc gtatccgggc
     1141 cggtgcggcg gacgaggaat gtgccggcgg ccgtgatccc gcttgtcaac ttgatcgagg
     1201 acaggccgcc ggccgaagca tcgtcgtcta cctccttgat caggatcacg acttcccggc
     1261 tctggccctt ctggccggcc cgcagcgtca gcttccagcc gtcgcgggat cgaaccagct
     1321 cctcaccggg cgcgcgccat tccatgcaat aggccaacca ggcggcagtc agcaatccgc
     1381 cggtgatgcc cgatttgccg gtgccgtcgc gcctggcgca ctcgatgatc acttcctcgg
     1441 tctcggagag gcaggagaga tgggatgggt tgtcgaagaa ctgcgccacc atttgccgcc
     1501 acgatcgcag ccgcgtccag gccatgtcgc tgatcttcag ttcggagccg ccggccttga
     1561 ccaggtgtac gagatagttg agcgcggtgg ccgggttggg gctggcggcg gagtcgaccg
     1621 tgatccgatc cacctgctcg gacagctcgt ccaggacggg gtcctgcgca aactcggggg
     1681 tcgtgcacca gacgaaatcg ggcaactcgg gtacgagcag cggcgtcgcg acgctggcga
     1741 ggcgctcgcc ccgtcctcgc acggagatga cctcgatccg ggtcggggcg tgcggcggtt
     1801 tgttcggccg ctcctcgata tcgacgccga ccttgagatt ctgcgcccat ccatgctgcg
     1861 acgataggat gacgacccgc gacgggctga tatcgggcag gtgcgtcacg agttgggcaa
     1921 catccggctg gtccacgttg ccgtcagtga tgacgaccag gttgaccgtc tgggtccgca
     1981 tcgaggcggc gtcggccacg gcgcgcgcaa gccgattgcc ggaccggcgc tcggctccaa
     2041 tctcgcccca cattcgattc aggaccttgc ggacatcgtc gggattgatc tggtcagcgt
     2101 cccagtgctc attccgggtg atagcgactg gttgaaggag atcggtcatt gtgcgttgcc
     2161 ctacggtcgt cgccaggtgc gccggtcatt ggccatcaaa agctcggcct cggctggtcc
     2221 ccaggtcccg gcttcgtagg tcggaatcgg catggtcgat ggttgatcgg cccagacatc
     2281 gaggatcggc tgcatgagcg accacgcggt gtcgatctcg tcgtcccggg cgaaaagcgt
     2341 cggatcgccg agcatggcgt cgagcagcaa gcggacatag gcatcaggtc cggcctggcc
     2401 gaacgaggcg ccgtaatcga agtccatctg cacgggctgg agtttggtgc cggcgcccgg
     2461 gaccttggcc gccacccgca gggaaattcc ctcgtctggc tgaatgcgca tggtcagcac
     2521 gttcggcggc agggtctggc cctcggtcag cttgaacatc aggtgcggca cgctcttgaa
     2581 ctggatcgca atctccgtca cgcgtcgtgg aagccgtttg ccggtgcgca ggtagaaggg
     2641 cactccgttc cagcgccagt tgtcgatctc cagcttcagc gcgacgaatg tctcggtccg
     2701 cgagtcagaa tcgacgcctt cttcctcgcg atacccgacc acctcggtcc ctgccacgta
     2761 cccatcggcg tattggcccc ggacaacgtc cttggcgacg cgatcgccgt ccggcgggac
     2821 gactgagcgg agcaccttga ccttctcgtc gcggagtgcg tcgccgttga aaaaggcggg
     2881 cggctccatc gcgacgaccg agaggagctg gagcatgtgg ctctggacca tatcccgcag
     2941 cgctccggcc tcttcgtagt aggtggcgcg gttctcgatg ccgagcgatt cggcgacggt
     3001 cacctgaatc tgatcgacat aatgtcgatt ccagaccggc tcgaagagga tgttggcgaa
     3061 gcggaaggcg aggacgtttt ggaccgtctc tttcccgagg taatgatcaa tccggtagat
     3121 ctgctgctcg gcaaagacgg cgttgatcgc ggcgttgaga tgctgcgcgc tggccagatc
     3181 gtgcccgaaa ggcttctcga tgacgatccg gtcccagccg ttggctcgcg ggttggagta
     3241 aatctcctgg cgcttgacga ggccggcatt cccgagtccg gccgagatct cctcgtagaa
     3301 gctcggcggg gtcgccaggt aatagagatg attgccgttg gtgccgcgct cggagtcgac
     3361 ggtctcgagc cgctcgcgga gccggccata aagctcggag ttgtcgaagt cgccgcgcac
     3421 gtagaacaag ccgcgcgcaa acgaatccca ggcatcttcg gtaaacgggc gagtcgcgtt
     3481 ctcgcgcacc gcgtcgcgca tctcgcagcg gaaatcgtcg tcgctccagt cccgccggga
     3541 gatgccgacg acggagaagc cgggtggaag cgggaccgag acgtagaggt catacagggc
     3601 cggcatcagc ttgcgtgagg tgagatcgcc ggacacgccg aagatgacca tcgcgcaggc
     3661 ttgcggattg cgctcggttt gcaggccgat ccgcagcggg ttaacgatct tgcttgccgg
     3721 cttgtgccgg acgcgctctg cggtgagcgt tccgccgccg gcggtgatga ccggggagtc
     3781 ggacatgaga gactccgttt cctgcacacg tcgtcggcgc cgggtgctgg ttgcgcccat
     3841 aaccagatct tacccctgga tctgctccgc ctcgcccgtg tcccagttcg catggaacac
     3901 gccggtggtg tcgatccgtt tgtacgtatg cgccccgaag tagtcccgct gcgcctggat
     3961 caggttggcc gggagccgct cggtgcggta actgtcgatg taatccaacg cggcgctcat
     4021 tgccggacaa gggagcccgt gcatccgggc caggacgact gcttcacggc aggcgttcga
     4081 tgcagagttg acagtatcca cgaattcggg cgccaggagc aggttgtcga gcgcggggtt
     4141 gttccggtac gcggtcatga tcggctcgag cagcttcgcc cggatgatgc agccgccggt
     4201 ccagattcgc gccagctccg acaggttcag tccgtacgag taggtgtccg atgccgcccg
     4261 cagcagcgcc atgccctgcg catacgacga gatctttgcg acgtagaggg cgtcctcgag
     4321 gacaccaatc aagcgctgct tctcggcgtc ggcgatctcc atggcagtcc cgggaccggt
     4381 caggatcttg ctcgccccaa cccggcgcgc cttgaatgag gagatcgatc gggcgaagac
     4441 ggccgcatcg atcacaggaa tcggcgtgcc gagatcgagc gcgttctggc tcgtccagcg
     4501 gccggttcct ttctgctcgg cggcatcgag gatcttctcg accaatgggt cgccggtgac
     4561 cgcgtccttc gtgccgagga ccttggcggt gatttcgatc aggaaggagg agagcttgcc
     4621 ctggttccag cggccgaaga catccgccat ctcggacgtg ctcatcccga gcgcccgctt
     4681 catgatgtcg tacgtctcgg cgatcagctg catgtcgccg tactcaatgc cgttgtggac
     4741 catcttgacg tagtggcccg cgccgcccgg accgcagtgg gtgacgcagg cgccgaactc
     4801 ggtcttggcg gcgatcgcgg tcagcatcgg ctcgaggatc tcgtaggcct tggccggacc
     4861 gcccggcatc aggctcggtc cccagagcgc gccttcctcg ccgccagaaa cgcccattcc
     4921 gacgaagtcg aacggcttcg actcgctctc cttcgcccgg cgctccgtgt cctggaagaa
     4981 ggagttgccg ccgtcgacga tcatgtcgcc ttccgagagc aacgggcgca gcgccgcaat
     5041 cgtctggtcg acgacctcgc ccgccttcac gagcatgatg agacgccgcg gccgccgcag
     5101 caccgccagc agctcgggca gcgccttgaa c
//
LOCUS       DATAOZ010000416         4727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1751142, whole genome shotgun sequence.
ACCESSION   DATAOZ010000416 DATAOZ010000000
VERSION     DATAOZ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751142"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VHR64_10720"
     CDS             <1..1081
                     /locus_tag="VHR64_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3303345.1"
                     /translation="AVLIGYSTKRIDNALDLLLKTLLIVATLAILVAAFIGYVLAGRA
                     LQPVAEITQLASQIDGDDLDARLNLDLPSDELGRLASTFDSMLERIDQAFTRQRQFTG
                     DAAHELRTPLSLMRSQIDLALTQAETPVEFREALSALDGDVSRMTSLVGMLLSLARAD
                     AGQLTPNREPVDLSDLARDVADQLEPIASENGLTIKTDLRSVTATVDADMIIQVLVNL
                     IDNAITNTPSGGTITVVVDKTTDIGHQAPNGRAPASQQLTTHASRLPSAVVTVSDTGI
                     GIPPQHLPRIFDRFYRVDTGRARSHGGIGLGLAICQAIVHAHHGTLTATSQPGRGSTF
                     TMTVPMDVRGEQASRRAGEQLAHRT"
     gene            1293..2288
                     /locus_tag="VHR64_10725"
     CDS             1293..2288
                     /locus_tag="VHR64_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter substrate-binding protein"
                     /protein_id="HEX3303346.1"
                     /translation="MLRFARGLIFALLMAALLPLGSLVATAHDGPHDATPVPATPTLP
                     VTVTDANGQQVTVTDVSRIIALNGATAEIIWDLGLGQNLVGVDVTATYPPEIADVPKI
                     GFGRQLSAEGIISLDPTVVIGDTTAGPPEVLDQIRQAGIPVVLYDQFTDLKAPFEKID
                     AIAAALGVTEAGDALKAQVQSEYDSAIALAKTATSHPRVLFLYIRGTSTLLIGGEGSG
                     ADTIIDAAGGIDAGTEAGVKGFAPITAEALVTAAPDVILVMTGGLQSIGGVDGLMQIP
                     GVAETPAGQNGAILEYEDQYLLGLGPRLGEMLTELTYNLHPELERPATPEATPGA"
     gene            2366..3388
                     /locus_tag="VHR64_10730"
     CDS             2366..3388
                     /locus_tag="VHR64_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX3303347.1"
                     /translation="MPASVLLPFLAALLLVAIVLAAGIGAVRVGPMQIIGILLHHLGI
                     ETDIAYTRQQDAVVWAIRIPRVLLATMVGAALAISGAVLQGIFRNPLADPSIIGVSSG
                     AAFGAVTAIVIGVTPFGLMTTPIAAFAGGIVTTLVVWNLSRRNGRVDTVTLILVGIAL
                     NSMIGAGMGMLNYVADDEQLRSVVFWSMGSLGGATWRNNLAILPLIALGLIVLPRKAR
                     DLNLLVLGEREARHLGVDVDRTRFLMIIVVALTTAAAVAFAGIIGFVGLIVPHLIRLV
                     SGPDHRVLLPASALAGASLLVLTDLLARTLVSPLELPIGIVTSLLGAPFFLFLLLRTR
                     WQHGVM"
     gene            3389..4186
                     /locus_tag="VHR64_10735"
     CDS             3389..4186
                     /locus_tag="VHR64_10735"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEX3303348.1"
                     /translation="MTDPQSARLILTDVSYSIGGAILLQGVSLAVQPGEVLVLVGPNG
                     AGKSTLMNVISGDLAPTSGEVMLDGRAIGSYRPNELALRRAVLPQQSLLQFAFTVREV
                     VEMGRTPHDDTADELRQHVQRALERTEMIPFADRVYPSLSGGEKARAQLGRVLAQETP
                     LLLLDEPTASLDIRHQQHVMHLAQDVAAEGGSVVAVIHDLNLAASAAHRIALLHRGRI
                     VADGPPWVVMDEKQLSEVYQCPIAVSKHPILHCPLILPLGRNGATSS"
     gene            4278..>4727
                     /locus_tag="VHR64_10740"
     CDS             4278..>4727
                     /locus_tag="VHR64_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX3303349.1"
                     /translation="MAEWRNWPGNVVATPARIERPGSIAAVQELVVDAARAGLSVRVS
                     GAGHSFTPICVTDGLLLDLADLSGIIGIDTDKQTATILGGTRIHQMGAPLFEAGLAIA
                     NQGDIDVQAITGAIGTGTHGTGRRFGSFSSTLVGCQIVTANGDLLTID"
BASE COUNT          758 a         1588 c         1515 g          866 t
ORIGIN      
        1 cgcggttctg atcgggtatt cgacgaagcg aatcgacaac gcactcgacc tgctccttaa
       61 gactctgttg atcgtcgcga cgctggcaat cctggtcgca gcgttcatcg ggtatgtgct
      121 tgccggccgc gcgcttcagc cggttgccga gatcactcaa ctggcaagcc agatcgacgg
      181 cgacgatctc gacgctcgac tcaatctgga tttgccgagc gacgagttgg gacgacttgc
      241 gtccacgttc gactcaatgc tggaacggat cgaccaggcc ttcacgcggc agcggcagtt
      301 caccggcgac gcggcacatg agctgcgcac cccgctctcg ttgatgcgca gccagatcga
      361 cctcgcgttg acccaggccg agacgccggt ggaattccgg gaggccctga gcgcgctcga
      421 cggcgacgtc agccggatga cgtcgctggt cggcatgctg ctctcgctgg cgcgggccga
      481 tgccggacaa ctgacgccaa accgggaacc ggtcgatttg tccgatctgg cccgggacgt
      541 tgccgatcaa ctcgagccga tcgcatctga gaatgggctg accatcaaga ccgacctgcg
      601 gtcggtcact gccacagtcg acgcggacat gatcatccag gtattggtca atctgatcga
      661 caacgcgatc acgaacaccc cgtccggtgg caccatcaca gtggtcgtcg acaagacaac
      721 ggacatcgga catcaggcgc cgaatggacg ggcgccggcg tctcaacaac tcacgaccca
      781 cgcctcccgt ctgccgtctg ccgtcgtcac cgtttccgac acggggatcg gtattcctcc
      841 ccagcacctg ccgcgcatct tcgatcggtt ctatcgggtc gacaccgggc gagcccgttc
      901 ccacggcggc atcgggctcg gccttgcaat ctgccaggcg atcgtccacg cccatcacgg
      961 caccctgacc gcgaccagtc aacccggtcg cggctcgacc ttcacgatga cggtgccgat
     1021 ggacgtacga ggcgagcaag cgagcaggcg agcaggcgag cagctggctc accgcacgta
     1081 atgctcatca gtcccgtgtc gtattcactc gaccccgtct gctcgcctgc tcgcctcaca
     1141 agcacccctg ccgcagttgg tagggctaca cacggatttg cgttgacatc ccccctccct
     1201 gtcccatagt tcagttggtg caccgatgtg gcccaggttt gcatagattc tgggcacgtt
     1261 catggcatga ttgacgtctg gaggaagtga ccttgcttcg atttgcccgt ggattgattt
     1321 tcgcgctgct gatggccgcg ctgctcccgc tcggctcgct cgtcgcgacc gcgcacgacg
     1381 gcccacatga cgcgaccccg gttcccgcca cgcccaccct gccggtcact gtgactgacg
     1441 ccaacggcca gcaggtcacc gtcaccgatg tcagccgaat catcgcgctc aatggcgcca
     1501 ccgccgagat catctgggat cttggcctcg gtcagaatct ggtcggcgtc gatgtgaccg
     1561 cgacctatcc gccggagatc gccgacgttc cgaagatcgg cttcggtcgc cagctttcgg
     1621 ccgaaggcat catctcgctc gatccgactg tcgtgatcgg cgacaccacg gctggcccgc
     1681 ccgaagtgct ggaccagatt cggcaggccg ggattccggt cgtactctat gaccagttca
     1741 ccgatctcaa ggcgccgttc gagaagatcg acgcgatcgc ggccgcactc ggcgtgaccg
     1801 aggcaggcga tgcgctcaag gcccaggtgc agagtgagta cgactcggcg atcgcgctgg
     1861 ccaagaccgc gacctcgcac ccgcgcgtgc tcttcctgta catccgcggc acctccaccc
     1921 tcctgatcgg cggcgaaggc tccggtgcgg acacgatcat cgatgccgcg ggcggaatcg
     1981 acgcgggaac cgaggcgggc gtcaagggat tcgcgccgat cacggctgag gccctggtca
     2041 ccgccgcgcc ggacgtgatc ctggtcatga ccggtggcct ccagtcgatc ggtggtgtcg
     2101 acggactgat gcagatcccc ggcgttgccg aaacgccggc cggacagaac ggcgcgatcc
     2161 tcgaatacga agaccagtac ctgcttgggc tcggtccccg actcggcgag atgctgaccg
     2221 agctgaccta caaccttcat cctgagctcg agcgaccggc tacgccggaa gcgactccgg
     2281 gcgcctagcc atgatctcgg cgggcactct gctcgatcgc cggcggcgcg cgtttcggtc
     2341 gcggtcggtc tcggcgtccc ggcgggtgcc cgcttcagtt ctcctgccgt tcctggctgc
     2401 gctcctgctc gtcgcaatcg tgctggcggc cgggatcggc gcggttcggg tcgggccgat
     2461 gcagatcatc ggcatcctgc tccaccacct cggaatcgag acagacatcg cctacacgcg
     2521 gcagcaagac gccgtggtct gggcaatccg aattccgcga gtgctgctgg cgacgatggt
     2581 cggcgcggca cttgcgatct ccggggcggt gttgcagggc atcttccgca atccgctggc
     2641 tgacccgagc atcatcggcg tctcgagcgg tgcggcgttc ggcgccgtca ccgcgatcgt
     2701 gatcggcgtc accccgttcg ggctgatgac cacgccgatc gcggcctttg cgggcggcat
     2761 cgtcaccacg ctcgtcgtct ggaacctctc gcgccgcaat ggccgggtcg acaccgtcac
     2821 gctgattctg gtcgggatcg cgctcaactc gatgatcggt gcggggatgg gcatgctcaa
     2881 ctacgtggcc gacgacgagc aattgcgctc ggtcgtgttc tggtcgatgg ggagcctggg
     2941 cggcgccacc tggcgcaaca acctggcgat tctgccgctg atcgcgctcg gcctgattgt
     3001 cctgccgcgg aaggcgcgcg atctcaatct gctcgtcctt ggcgagcggg aagcgcgcca
     3061 cctgggcgtg gatgtcgatc gcacccgttt cctgatgatc atcgtggtcg cgctgaccac
     3121 ggcggcggcg gtcgcattcg ccgggatcat cggcttcgtc ggcctgatcg tgccgcacct
     3181 gatccggctg gtctccgggc ctgaccaccg cgtactgttg ccggcgagcg cgctggcggg
     3241 cgcatcgctg ctggtgctaa ccgatctgct cgcacggacg ctggtttcgc cgctggaact
     3301 gccaatcggg atcgtcacct ccctgctcgg ggcgcccttc ttcctcttcc tgctgctccg
     3361 cactcgctgg caacacggcg tcatgtagat gaccgacccg cagtctgccc gtctgatcct
     3421 gaccgacgtc tcgtattcga ttggcggcgc gatcctgctc cagggcgttt cgctggcggt
     3481 ccagccgggc gaggtgctgg tgctggtcgg tccgaacgga gccggcaagt cgaccttgat
     3541 gaacgtgatc tcgggcgatc tcgcgccgac gtcgggtgag gtgatgctcg acggccgcgc
     3601 gatcggctct taccggccga atgagctggc gctacgacgg gcggtattgc cgcagcaatc
     3661 gctgctccag ttcgcattca ccgtgcgcga ggtcgtcgag atgggccgga ctccgcacga
     3721 cgacacggcc gacgagcttc gccagcatgt ccagcgcgcc cttgagcgga ccgagatgat
     3781 cccgttcgcc gaccgcgtct atccctcgct ctcgggaggc gagaaggcgc gggcccagct
     3841 cggccgtgta ctggcccagg agactccgct gctgctgctc gacgagccga ccgcgtcgct
     3901 cgatatccgt caccagcagc acgtcatgca cctcgcgcag gatgtcgcgg ccgaaggcgg
     3961 ctcggtcgtc gcggtcattc acgacctgaa tctggccgca agtgccgcgc accggatcgc
     4021 tctcctccat cggggccgga tcgtggccga tggtccaccg tgggtcgtaa tggatgagaa
     4081 gcagctgagc gaggtctacc aatgtccaat tgcggtcagc aagcacccga tcctgcactg
     4141 cccgctcatc cttcccctcg gacgaaatgg cgcgacgagc tcctaggctc gaacggatag
     4201 cgctgggcca aaatgccttg cgtcgacgcc gtgccgtatg ctgccgccac tcgatggttc
     4261 gcgtgagggg caggccgatg gctgagtggc ggaattggcc gggaaatgtc gtggcgacgc
     4321 cggcgcggat cgagcggccg ggatcgatcg cggcggtgca ggagctcgtc gttgatgcag
     4381 cgcgggccgg gctgtccgtg cgggtctccg gcgcggggca ttccttcact ccgatctgcg
     4441 tgacggacgg gctgctgctc gacctggccg atctgtccgg catcattgga atcgacaccg
     4501 acaagcagac cgcgacaata cttggcggca cgcgcatcca ccagatgggc gcacctctct
     4561 tcgaggcggg gctggcgatc gcgaatcagg gcgatatcga tgtccaggcg atcaccggcg
     4621 cgattgggac cggcacgcat gggacagggc gacgattcgg ctccttctcc tcaacgttgg
     4681 tcggttgcca gatcgtgacc gcgaacggcg atctgctgac gatcgac
//
LOCUS       DATAOZ010000417         8729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8852051, whole genome shotgun sequence.
ACCESSION   DATAOZ010000417 DATAOZ010000000
VERSION     DATAOZ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8852051"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..3422)
                     /gene="mfd"
                     /locus_tag="VHR64_10745"
     CDS             complement(6..3422)
                     /gene="mfd"
                     /locus_tag="VHR64_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX3303350.1"
                     /translation="MNLIHIRRAVDAAVRAHQPSLLAEPVLSGARPAVLASLARAHSS
                     AVLILTGRSDQAEQICPRIAAYLPAEQEPRLWDTPHSTPYEQLPYEHQSAAERVRVLT
                     GLIDGDAPIIVASARNLMHLTIAPDDLRSMRRTVRVGERINADALIHWATEMGYQRTP
                     LVFEPGTIAQRGGVIDLFPPDVDDPIRLDLFGDEIESIRIFDAETQRSRTPLTELSLL
                     PPVDIPTFRAEDAARGLRELSDRSLRPEVRAEWHALIDRVELGQLPAGMDLVTAYFGQ
                     MTSLLTYLPADTLVLFDDRHSVSAVMREIAGHAEELHAASIANGELPKNLEAPYVDPR
                     HLESALDRFECHDLRAVATDEVSSDGRVFRVAPLFAGRTDRLVNDVAALLGEGWAVQI
                     ATDQPRRLTEIFEEQDVFPRKIRANTGEAALPPGTLDIRVADASTGWSLPESKVLLLT
                     DLEIFGFSKQTRRRRGRSVAENVAFAESLTPGEYVVHVDQGVARFVGLTRTEAAGVER
                     EYLLLEYARGDKLYVPVDQSDRVSRYSSGGLEPRLTRLGTGEWQKAKSRVRRAVREMA
                     FELIQLYAARESGEGIQYPPNTTWDEELAESFPYVETTDQQSAIVDVTGDLETDRPMD
                     RLVCGDVGFGKTEVALRAAFKVVNAGKQVAVLVPTTVLALQHFATFTQRLAAFPVRVE
                     MLSRLKSRGEQGEIVNGLAEGGVDIVIGTHRLVQNDIRFQDLGLVVIDEEQRFGVRHK
                     EFLKRLRTQVDVLTMSATPIPRTLHMSLAGIRDISIINTAPQERLPIRTFVTDYNDSL
                     VREVILREIDRGGQVYFVHNRVHSIDHLAAHLRELVPEARFGIGHGQMEEEVLEDVIL
                     GFVRHEFDVLICTTIIESGIDIANVNTVIIDDADRFGLTQLYQLRGRVGRGSHRAYAY
                     LLHKPHKALSEIAQARLDAIQDATELGAGMQVALRDMEIRGAGNVLGAEQSGNIADVG
                     YDLYIRLLSQAVEEIRQGRPFAEPTPLTLDLPLTALIPASYIGDVELRLNTYRRVASA
                     ETLAEVNHVEAELRDRFGEPPEEVEHLLALIRVRIRCAALGIQSVVEREREIVLRPVE
                     TASLKGRGLERVLGSALRFTPNSVRIRMSELEIPWEQALDAVIAAIEQSQ"
     gene            3531..4112
                     /locus_tag="VHR64_10750"
     CDS             3531..4112
                     /locus_tag="VHR64_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEX3303351.1"
                     /translation="MHFSPTKMTPALTGLTGAIAMFVAVLASFIISGRSNDGDVVIIS
                     DPAAFEIAVEIRGAVQSPGVYRLSQDARLDDLLTAAGGVTADADLAPHNLARRLNDAE
                     VVVIERIGSATPVIAVASEAPPQSISYRININTATNAELETLPGIGPVLAQRIIDDRT
                     ANGPYRDITDLTRVPGISEKLIDEIRAQITVGP"
     gene            4121..5593
                     /locus_tag="VHR64_10755"
     CDS             4121..5593
                     /locus_tag="VHR64_10755"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX3303352.1"
                     /translation="MLAAAVLAGFAFGWAVVPIIAAVAFLARIGRATGWVLGALIIGA
                     VVLGAIRSAPSRGHELDASWITVSEQTGQIAGSVVDDGRMQRFIVRIDDGHRLCARAF
                     SRADLGRGDRIRLQIDPDATGPISDGYAAYLRSRGCDWSGTAGRITLLRHGTGVLRTF
                     DSVRSDIAMQLVDWVPGDSGALLAGLVVGDDSLMSDETMDAFERTGTLHVVAISGSNL
                     TLLASLLLVASVWSPRRWLTDLAAMAVIWLYVLVGGAGPPTIRAGFLATAGTGARAFG
                     RPPDLMTLSLQVAAIQALIWPSSVLGLSYQLSTVAIFGVLVATTRRSFEGVGGAIWIV
                     VLTTIVVNLVSLPILPAASRPSLLQSLLANTIIAPLVSLAFVLGLLAIAVGVVVPVIG
                     QSIAVVAGEINGATISIVRAIGAWDWLPGSLAWDGSNAPPRLLLAGAIIVMLGVSKDF
                     RRGLRDARVRLAFADEATGMLMLGSGVGACVGVVIVALAR"
     gene            5631..5707
                     /locus_tag="VHR64_10760"
     tRNA            5631..5707
                     /locus_tag="VHR64_10760"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5665..5667,aa:Glu,seq:ttc)
     gene            5826..6800
                     /locus_tag="VHR64_10765"
     CDS             5826..6800
                     /locus_tag="VHR64_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX3303353.1"
                     /translation="MDVILPVAGLGTRLRPQTWSKPKPLVSLAGKTILEHVLDRVLPT
                     HPEKIVFITGYLGSHIEKWARENITIPTEFVKQTEMRGQTDAIIRARDISRDDALILF
                     PDMLFEADFSIIHKSDADVIMFTKEVEDPSALGVAVLEDGRIVKLVEKPKEMISNLAV
                     IGIYYVRSMPALYEAIDEQMERGIKTKNEFFIADAIQIMIDKGAKVISAPVTLWEDCG
                     NAKSLLSTNRILLARDEERVETRGNSVIIHPSVVDESAHLERSIVGPFASVGENCRIV
                     DSLVTDSIIEDDASVECSQLTGSIIGFGASVRGRAVSINVGDVSTIEL"
     gene            6797..7717
                     /gene="prmA"
                     /locus_tag="VHR64_10770"
     CDS             6797..7717
                     /gene="prmA"
                     /locus_tag="VHR64_10770"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX3303354.1"
                     /translation="MSDQPGRWYELTTWADSEAIESVAELFARYGYNEGVSIEEPFLQ
                     EQDGDNLRIDMSKPAVIRTYIPAESYDPETVDRIRDGLWHLGQMRSVGELTVTERSEE
                     DWASAWKEHYRPLRASDRVVIRPPWFEYEAQENDIVLVLDPGMAFGTGMHPTTRLSLY
                     QIEKLVQPGQSLFDVGTGSGILAIAAMRIGASPIDAVDIEPMSVRVAKGNLDLNDAGN
                     RITLDVGSADWAGGKSYDVVVANIIARVLISIADDLKAATKPGGLLLLSGIIEAKETE
                     TRERFDAVGFEMLERNQIEDWISLAYRLPG"
     gene            7795..>8729
                     /locus_tag="VHR64_10775"
     CDS             7795..>8729
                     /locus_tag="VHR64_10775"
                     /inference="COORDINATES: protein motif:HMM:NF039894.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX3303355.1"
                     /translation="MPVVSPISLQRVDLAPSVPGVIEAIEPESLADQIGLLKGDRITT
                     VNGVPVLDALDFQFHAQSESVRIEILRGDRRRRFDLKLAGDEFWGITFADPTFDGIRV
                     CENACPFCFIKQIPKGMRRSLYVMDDDYRYSMLYGSFVTLTNLSDRDWARIEEQRVSP
                     MHVSVHATNPELRVALVGNPKGAEIMEDLNRLRRNNIDFHAQLVVCPGVNDGEEMDRS
                     VRGLAALDNCLSIAAVPVGLTRHGMERQSKQVRLSRTCMRLLPGKQITVRRYEPEEAR
                     AVIRQAEAWQRHFRAERGETFLHLGDEFYLMCGEPV"
BASE COUNT         1542 a         2688 c         2871 g         1628 t
ORIGIN      
        1 caggatcatt gagactgctc gatcgcggcg atcaccgcgt ccagtgcttg ctcccatgga
       61 atctcgagct cggacatgcg aatccggact gagttcggcg tgaagcgaag ggcactgccg
      121 aggacccgct ccagtcctct ccctttcaaa ctggcggtct cgaccggtcg caggacgatt
      181 tcccgctctc gctcgacaac ggactggatg cccagcgccg cgcaccggat ccggacgcga
      241 atcagcgcca gaaggtgctc gacctcctcc ggcggctcgc cgaaccgatc gcgtaactca
      301 gcctcgacat ggttgacctc ggccaacgtt tccgctgagg cgacgcgccg ataggtattg
      361 agccggagct cgacgtcgcc gatgtagctc gccggaatga gcgcagtcag ggggaggtcg
      421 agcgtgagcg gtgtcggctc tgcgaatggt cggccctgcc gaatctcctc cacggcctgg
      481 ctgagtagcc ggatgtacag gtcgtatccg acgtcggcga tgttcccgct ctgctccgcg
      541 ccgagcacgt tgccggcgcc gcggatttcc atgtcgcgga gggcgacctg catgccggcg
      601 cccagctccg ttgcgtcctg gatcgcgtcg agacgcgcct gggcaatttc gctcaacgcc
      661 ttgtgtggct tgtgcaggag gtacgcatag gcgcgatgcg acccacgacc gacccgccca
      721 cgcaactggt agagctgggt cagcccgaat cggtcggcgt cgtcgatgat gaccgtgttg
      781 acgttcgcga tgtcgatgcc cgattcgatg atcgtcgtgc agatcagcac gtcgaactcg
      841 tgccgcacga aaccgaggat gacatcctcg aggacctctt cctccatctg accatgaccg
      901 attccgaagc gcgcctcggg gacaagttcc cggagatggg cggccaggtg atcgatcgag
      961 tggacgcggt tgtggacgaa gtagacctga ccgccgcgat cgatctcacg caggatcacc
     1021 tcgcgcacca gcgagtcgtt gtagtcggtg acaaaggtgc ggattggcag ccgttcctgc
     1081 ggcgcggtat tgatgatgct gatgtcccgg atcccggcca gcgacatgtg gagcgtgcgc
     1141 ggtattggcg tcgcgctcat ggttagaacg tcgacctggg tccgcagccg cttcaggaac
     1201 tccttgtggc ggacaccaaa gcgctgctcc tcgtcgatca cgacaagacc gagatcctgg
     1261 aacctgatgt cattctggac gaggcgatgg gtgccgatca caatgtcgac gccgccctcg
     1321 gccaaaccat taacgatctc gccctgctcg ccacgcgact tgaggcggga gagcatctcg
     1381 acgcgcaccg ggaatgcggc caggcgctgt gtgaacgtcg cgaagtgctg cagcgcaagc
     1441 accgtcgtcg ggaccagcac ggcgacctgc ttgccggcgt tgaccacctt gaaggcggcg
     1501 cgcaacgcga cttcggtctt gccgaatccg acgtcgccgc agacgaggcg atccatcggc
     1561 cgatcggtct caagatcgcc ggtcacatcg acaatcgccg actgttgatc cgtcgtctcg
     1621 acgtatggga atgactcggc cagctcctcg tcccaggtcg tgttcggggg atattggatg
     1681 ccctcgccgc tctcgcgggc ggcgtagagc tggatcagct cgaatgccat ttcgcggacc
     1741 gcgcggcgaa cccggctctt ggccttttgc cattcaccgg tgccgagccg ggtcaatcgg
     1801 ggctcgagcc ccccgctcga ataacggctg acgcgatcgc tttggtcgac cggaacgtag
     1861 agcttgtcgc ctcgggcgta ctcgagcagc aggtactccc gctctacccc ggcggcctcg
     1921 gtgcgggtca gaccgacgaa ccgggccacg ccctgatcga cgtggacgac gtactcgccc
     1981 ggcgtcaggc tctcagcgaa ggcaacattt tcggccaccg agcggccgcg gcgccggcgc
     2041 gtttgcttgc tgaagccgaa gatctcgagg tccgtcaaga gcagcacctt gctctccggc
     2101 agtgaccaac cggtagaggc gtcggcgacc cgaatatcga gcgtgcccgg cgggagcgcg
     2161 gcttcgccgg tgttcgcgcg aatcttgcgc gggaagacat cctgctcctc gaagatctcg
     2221 gtcagccggc gtggctgatc ggtcgcgatc tggaccgccc agccctcgcc gagcaacgcg
     2281 gcgacgtcgt tcacgaggcg atcggtacga cccgcgaaga gcggcgcgac gcggaaaacg
     2341 cgaccgtcgc tgctgacttc gtcggtcgcg accgctctca ggtcatggca ctcgaatcga
     2401 tcgagcgccg attccaggtg cctggggtcg acgtacggag cctccagatt tttcggcagt
     2461 tcgccattcg cgatagatgc cgcatggagt tcttcggcgt gcccggcgat ctctcgcatg
     2521 accgctgaga ccgagtgacg atcgtcgaac aggacgagcg tgtcggccgg caggtaggtc
     2581 aaaagcgagg tcatttggcc gaagtaggct gtgacgaggt ccataccagc ggggagttgc
     2641 ccgagctcga cccggtcgat cagcgcgtgc cattcggcgc ggacctccgg ccggagcgag
     2701 cgatcgctga gctcgcgcag gccgcgcgcc gcatcctcgg cccggaaggt cgggatgtcg
     2761 accggtggaa gcaggctcag ctcggtcagc ggcgttcgcg accgctgtgt ctccgcatcg
     2821 aagatgcgaa tcgattcgat ttcgtcgccg aacaggtcga gccggattgg atcatccacg
     2881 tcaggcggga agagatcgat gacaccgccg cgctgggcga tcgtgcccgg ctcgaagacc
     2941 aacggcgttc gctggtagcc catttcggtt gcccagtgga tcagggcatc ggcgttgatg
     3001 cgctcaccga cgcggacggt tcgacgcatc gagcgcaggt cgtccggcgc gatcgtcaag
     3061 tgcatcagat ttcgcgccga cgccacgatg atcggcgcat ctccatcgat aagcccagtc
     3121 aagacgcgca cccgctcggc cgcggactgg tgctcgtacg ggagttgctc gtagggcgtg
     3181 gagtggggcg tgtcccagag gcgcggttcc tgctcagccg gaagataggc ggcgatgcgc
     3241 gggcagatct gctccgcctg gtcagagcga ccggtcagga tcagcaccgc gctggaatgg
     3301 gcccgggcaa gcgatgccag cacggccgga cgtgctccgg acaagaccgg ctccgcgagc
     3361 agggacggtt ggtgtgcccg cactgcggca tcgacggcgc gccgaatgtg gatcaggttc
     3421 aagtgtggac gctcccgggt tcagcgcact gcgcggccgg tgagtctagc acggtgttgc
     3481 accttccgat tcgaattgcg atcatcctgt gcacaaagga cgcaccagac atgcactttt
     3541 cgccgaccaa gatgacccct gcgctcaccg gtctgacggg cgcaatcgcc atgttcgtgg
     3601 ccgtcctcgc gagctttatc atcagcggcc ggtcaaacga cggcgatgtt gtcatcatct
     3661 ccgatccggc tgcgttcgag atcgcggtcg agattcgtgg cgcggttcag tcgcccggtg
     3721 tctacagatt gtcgcaggac gcgcgtctcg acgatttgct gaccgcggct ggtggcgtta
     3781 ccgccgacgc ggatcttgcg ccacacaacc ttgcccgtcg cctgaacgac gccgaggtcg
     3841 tcgtgatcga gcgaatcggc tcggcgacgc cggtcatcgc ggtggcaagc gaggcgcctc
     3901 cgcagtcaat cagctaccgg atcaacatca ataccgcgac caatgccgag ctggaaacgc
     3961 taccgggaat tggtcccgtg cttgctcaga gaattatcga tgatcggacc gccaacggac
     4021 cgtatcgtga catcacggac ctgacgcgtg ttccgggcat ttccgaaaag ctgattgacg
     4081 aaatccgggc acagatcaca gtcggcccat gactggggca atgctcgccg ctgcagtcct
     4141 ggcagggttt gcctttggtt gggccgtcgt cccgataatt gcggcagtcg cattcctggc
     4201 tcgcatcggc cgcgcgacag ggtgggtgct cggtgcgtta attatcgggg cggtggttct
     4261 cggggcgatc cgatcagctc ccagtcgcgg gcatgaactc gatgcatcat ggatcacagt
     4321 aagcgagcaa actgggcaga tcgccggatc ggttgtcgat gacggacgaa tgcagcggtt
     4381 catcgttcgg atcgacgacg gacatcgcct ctgcgcccgc gcgttcagcc gtgccgatct
     4441 ggggagaggc gaccgcatca ggctgcagat cgatccggac gcgacagggc caatctcgga
     4501 tggctacgct gcgtacctgc gctcacgagg gtgtgattgg agcgggacgg ctggtcggat
     4561 cacgctgctc cggcatggga ctggcgtcct tcgcacgttc gactcggttc ggtcggacat
     4621 cgccatgcag cttgtcgatt gggtgccggg cgattccggc gcgctgcttg cgggactggt
     4681 cgtcggcgat gactcgctca tgtcggatga gacgatggac gcgtttgagc gaacggggac
     4741 gcttcacgtc gtggcgatca gcggctcgaa tctgacgttg ctggcttcgt tgttgctggt
     4801 ggcaagcgtg tggtcgccac gccgatggct aaccgacctc gcggcaatgg ccgttatctg
     4861 gctctatgta ttggtcggcg gggcgggtcc gccaacgatt cgggcaggat tcctggcgac
     4921 ggcgggcacc ggcgcgcggg cattcggccg gcctcccgat ctgatgacgc tctcgctgca
     4981 ggtcgcggcg atccaggcac tgatctggcc gtcatctgtg ctggggctct cgtatcagtt
     5041 gtcgacggtc gcgattttcg gtgtcctggt ggcgacgact cggcggtcgt tcgagggagt
     5101 cggcggcgcg atctggatcg tcgtgctgac cacgatcgtc gtcaacctgg tctcgctgcc
     5161 aatcctgccg gcagcttccc gaccatcgct cctgcaatcg ctgctggcca acacgattat
     5221 tgcgccactg gtcagccttg cgttcgtcct gggcttgctg gcaatagcag ttggtgtggt
     5281 cgttccagta attggccagt cgatcgcggt ggtcgccgga gagatcaacg gagcgacgat
     5341 ctccatcgtg cgggcaatcg gcgcttggga ttggcttccg ggctcgctgg cttgggacgg
     5401 atccaacgcg ccgcccaggc tgctcctcgc gggagcgatc attgtcatgc tcggcgttag
     5461 caaggacttc cgccgtggcc tgcgcgatgc acgagttcga ctggcgttcg ctgatgaggc
     5521 caccggaatg ctcatgttgg ggtcgggggt cggcgcatgc gtgggagtcg tgatcgtggc
     5581 gctcgcgcgt tgacaatttc ggcgtgcagg cggtaccttt cttcggctag gcccccatag
     5641 tctagtggcc caggacgtca ccctttcaag gtgaagatcg ccggttcgaa tccggctggg
     5701 ggtaccactt ccgaagcccg agtccccctc ctcaaggaca gtgccgtcat gcagcggtgc
     5761 gcgcccggca ccatggtagg atcacgctac gacagctgat tcttcacatg cgaggcgatc
     5821 gtccgatgga tgtcattctc cccgtcgccg ggctcgggac caggctgcgc ccgcagacct
     5881 ggagcaagcc caaacccctg gtttctctcg ccggcaagac cattctcgag catgttcttg
     5941 accgcgttct gcccacccac cccgagaaga tcgtcttcat caccggatac ctgggcagcc
     6001 atatcgaaaa atgggcacgc gagaacatca cgatccccac cgagttcgtc aagcagacgg
     6061 aaatgagggg ccagacagac gcgattatcc gcgcgcgcga catctcccgg gatgatgcgt
     6121 tgatactctt cccggacatg ctgttcgagg ccgatttctc gatcatccac aagtccgatg
     6181 ccgatgtgat catgttcacc aaggaggtag aggacccctc tgcgctcgga gtcgcggtcc
     6241 tcgaggatgg ccgaatcgtc aagctcgtcg agaagccaaa ggaaatgatt tccaacctgg
     6301 ccgtgatcgg catctactac gtccgctcga tgccggcgct ctacgaggcc atcgacgagc
     6361 agatggagcg ggggatcaag acgaagaacg agttcttcat cgctgacgcg atccagatca
     6421 tgatcgacaa gggggcgaag gtgatctccg cgccggtcac gctctgggag gactgcggca
     6481 acgccaaatc gctgctctcg acgaaccgga tcctgctcgc gcgggacgag gaacgggtcg
     6541 agacgcgcgg aaacagcgta atcattcacc cgtcggtggt ggatgagtcc gcgcacctcg
     6601 agcgctcgat cgtcgggccg tttgccagtg tcggggagaa ctgccggata gtcgattcgc
     6661 tggtgaccga ttcgatcatc gaggacgacg cgagcgtcga gtgctcgcag ttgaccgggt
     6721 cgatcatcgg attcggggcg tcggtacgcg gtcgcgcggt ctcgatcaat gtcggcgacg
     6781 tgagcacgat cgagctgtga gcgaccaacc cggccgctgg tacgagttga caacctgggc
     6841 ggacagcgag gcaattgagt cagtcgccga gctgttcgcg cgatatggtt acaacgaggg
     6901 cgtctcgatc gaggagccat tcctgcagga gcaagatggc gacaacctcc ggatcgacat
     6961 gtcgaaacca gccgtcattc ggacctacat ccctgctgaa agctacgacc cggagacggt
     7021 cgatcgcatc cgggacggct tgtggcacct cgggcaaatg cgctcggtcg gggagctgac
     7081 cgttacggag cgatctgaag aagactgggc atcggcttgg aaggagcact accggccgct
     7141 tcgggccagc gaccgcgtcg tgattcggcc gccctggttt gagtatgagg cacaggaaaa
     7201 cgacatcgtc ctggtgctgg atccgggaat ggccttcgga accggaatgc acccgacgac
     7261 gcggctcagc ctctatcaga tcgagaagct cgtgcagccg gggcaatcgc tcttcgacgt
     7321 cggtaccggc tcgggcatcc ttgcgatcgc ggcaatgcgc attggcgcgt ccccgatcga
     7381 cgcggtcgat atcgagccga tgtcggtccg ggtcgcgaag ggcaatctcg acctcaatga
     7441 cgccggcaat cgaatcacgc tcgacgtcgg ctcggccgat tgggcgggcg gcaagagcta
     7501 cgacgtggtc gtggccaaca tcattgcccg ggtgctgatc tcgattgccg atgatctgaa
     7561 ggcggccacg aagccgggcg gtctgctgtt gctgagcggc atcatcgagg ccaaggagac
     7621 cgaaacacgc gagcggtttg acgcggtcgg ctttgagatg ctggagcgca accagatcga
     7681 ggactggatc tcgctggcct atcggctgcc gggttgagcc tcataatgta gtaaactcat
     7741 ccgttggccg cgggcgcgcg gtcgccgccc tttgcgaaag ctcccagata ccgaatgcca
     7801 gtcgtcagcc ccatatccct gcaacgggtc gaccttgctc catccgtgcc cggcgtgatc
     7861 gaggccatcg agccggagtc gcttgccgac cagatcgggt tgctcaaagg cgaccggatc
     7921 accacggtca acggcgtgcc ggtcctcgat gcgctcgatt ttcagtttca cgcccagtcc
     7981 gagtcggtcc ggatcgagat cctgcgcggc gatcggcggc gccgattcga cctgaaactg
     8041 gcgggcgacg agttttgggg catcacattc gccgacccga catttgacgg catccgcgtc
     8101 tgcgagaacg cctgcccgtt ctgcttcatc aagcagattc cgaagggcat gcggcggtcg
     8161 ctctatgtga tggacgacga ctaccgctat tcgatgctct acggcagctt cgtgacactg
     8221 accaacctgt ccgaccggga ctgggcgcgg atcgaggagc agcgggtcag cccgatgcat
     8281 gtctcggtgc acgccaccaa ccccgagctg cgggtcgcgc tcgtcggaaa tccgaagggc
     8341 gccgagatca tggaagatct caatcgcctg cgccgaaaca acatcgattt ccacgcgcag
     8401 ctcgtggtgt gtcccggcgt aaacgacggc gaggagatgg atcgcagcgt tcgcgggctg
     8461 gccgcactcg acaattgcct gtcgatcgcg gcggttccgg tcggtctgac gcggcacggg
     8521 atggagcggc agagcaagca ggtccgcctc tcgcgtacat gcatgcgact gctgcccggc
     8581 aagcagatca cggtccggcg atacgagccg gaagaggcga gggcggtcat ccggcaggcc
     8641 gaggcgtggc agcgtcattt ccgggccgag cgcggggaga cattcttgca tctcggcgac
     8701 gagttctacc tgatgtgcgg cgagccggt
//
LOCUS       DATAOZ010000418         3806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3587288, whole genome shotgun sequence.
ACCESSION   DATAOZ010000418 DATAOZ010000000
VERSION     DATAOZ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3587288"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VHR64_10780"
     CDS             <1..510
                     /locus_tag="VHR64_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3303356.1"
                     /translation="EMSGPLPSGFNPNGYSIPGKRPSRTYDDGDVVELGNRTLEVIHA
                     PGHSRGGLVLLDRANRALYSTDVVYLRSLYLLNPDSSVPDYVTTLERLSELVPELVRL
                     YPSHGPSPISPQLIPQMLDGMRLIAGGRKPDQVEPSPAIEGGRPDVTGRSELRIHEFG
                     EFRVLVAVW"
     gene            complement(542..1183)
                     /locus_tag="VHR64_10785"
     CDS             complement(542..1183)
                     /locus_tag="VHR64_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3303357.1"
                     /translation="MNCCRAQALDTEFGKKIAAMNLRQYRRRGPSKTTRLLLSALRAE
                     GVAGRTLLDIGGGVGAISHELLRSDLAHATSVDAAGAYIEAARSEAERQGHLDRLSLH
                     QGDFVELAGDIRPADIVTLDRVICCYPDMPALVGASVAHAGHVYGLVYPRNAWWTRLG
                     VRMINLVFRIRRHSFRTFIHSPEAVDALVVARGFQLRFRRLTGVWQVVVYART"
     gene            complement(1593..3806)
                     /locus_tag="VHR64_10790"
                     /pseudo
     CDS             complement(1593..3806)
                     /locus_tag="VHR64_10790"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT          678 a         1247 c         1126 g          755 t
ORIGIN      
        1 gagatgagcg ggccgctgcc atccggcttc aatccgaacg gctactccat tcccggcaag
       61 agaccgtcac ggacctatga cgacggtgac gtcgtcgaac taggcaaccg gacacttgag
      121 gtgatccatg cgcctgggca ctcgcgcggt gggctggtgc ttctcgaccg ggccaataga
      181 gccctttatt ccacggatgt cgtatacctc cgctccctgt atctgctcaa tccggattcg
      241 tcggttcccg actatgtcac cacactggag cgcctgtccg aactcgttcc agagctcgtt
      301 cggctctacc catcacacgg gccgagcccg atctcgccac aactgattcc gcaaatgctc
      361 gatggcatgc gtctgatcgc gggcggacgc aaacccgatc aggttgagcc aagcccggca
      421 atcgagggtg gccgaccgga cgtgacgggt cggtccgaat tgcgaatcca tgagttcgga
      481 gagttcaggg tgcttgttgc cgtttggtga cgaacctgtc gatggggagc ccgtaccagg
      541 ttcacgttcg agcgtagacc acgacctgcc agacaccagt cagacgccga aatcggagct
      601 ggaatcctcg cgcgacgacg agggcgtcga ccgcctcggg cgaatgaatg aatgtccgga
      661 aggagtgtcg ccgaatccgg aacaccaggt taatcatccg cactcccagt cgcgtccacc
      721 atgcatttcg cggatagacc agcccataga cgtgcccggc gtgagcaacg gacgcaccga
      781 ccagcgccgg catatccgga taacagcaaa tgacacggtc gagcgtgacg atatcggccg
      841 gccggatgtc gccggcgagt tcaacgaagt cgccctgatg gagtgacagc cgatccaggt
      901 gtccctgtcg ttcggcttcg cttctggccg cctcgatgta cgcaccggcg gcatcgaccg
      961 acgtcgcgtg cgcgagatct gaccgaagaa gctcatgcga gatcgccccc acaccgccac
     1021 cgatgtcgag caacgtcctg cccgccactc cttcggctcg taatgcgctc agcaacaagc
     1081 gcgtggtttt tgatggcccg cgccggcggt actggcgaag gttcatagcc gcaatcttct
     1141 tgccaaactc ggtgtccagg gcctgggctc ggcaacaatt catctcgaac ctcctggtac
     1201 ctcatcacac cgaagaatcc acatcacctt gtggaggctc cgcgcgtcgg tgccaatgac
     1261 aatgggcttg acgatttgcc gacattgtgc ccaaaggata tgagaacggg acgggcattg
     1321 tgcccgtccc gttcttctgt tcggatgctg tgtggcgcgg actagttgat gtcctgcgcc
     1381 tcgagatcct cgacggtcga gacggaatag ccgtccgcct cgtcgccggt gacctcgtcg
     1441 ctcggcagcg cgccggactg catcaattcg gcgtagtcct cgtcggtagc ctgctccgcg
     1501 tccacaaccg cggtcgcagc gcggccctcc tcgatagcct cgagggtgac cgtgtccaga
     1561 ttcgagagat cgagaccacc ggtcacgccg taaccggacc cggccggaat cagcttgccg
     1621 atgatcacgt tctccttgag gccgcgcagc gaatcgacct tgccgttgat agcggcttcg
     1681 gtcagtacgc gcgtggtctc ctggaacgac gcggccgaga ggaacgaatc ggtctcgagc
     1741 gaggccttgg tgattccgag caggaccggg atcgccgttg ccggctcgcc gccctgcgca
     1801 aggacttcct cgttgatccg attgaactcc agccggtcga cgcgctcgtc ctgcagcaga
     1861 tcggtgtcgc ccgggtagac gatgctgacc ttgcggagca tctgccggca gatgacctcg
     1921 atgtgcttgt cgttcgtgtt cacgccttgc gacttgtaca ccttctgcac ttcgtcgatg
     1981 acgtacttct gaacggcatc gggaccgagg gtgacgagca cctgctgcgg gtccttggcg
     2041 ccatcggtca tttgctgtcc gggcgtcacc agctcaccgt cttcgacgaa gatttggtag
     2101 acgtggccga cctgatgctc ttccgtctct tcccgctcgt tgcggatcag gattgcgttg
     2161 ccatccacgt aggcaacccc atcgatcggc gcgagcttct tctcttcagt tgggccaacc
     2221 gcgaccaggg tcttgtcctt gattaccggc gtagcagtcg ccacggccgg atcccagccc
     2281 tttgcgaggt caaccgttgc ggtgtcctgc tcgacgctgg tgactttcag aatccgccct
     2341 gtctcggtct gctcgataga gacgacgccc tcgcgctcgg ccaggatagc cgcgcccttc
     2401 ggcacgcgcg cctcgaacag ctcctcgacg cgcgggagac cagtcgtgat gtccgcccgc
     2461 gccacaccac cggtgtggaa tgtgcgcatc gtcaactggg tacccggctc gccgatcgac
     2521 tgtgcggcga tgatacccac cgcttcgccc agatcaacca gcttgccact ggccaggttc
     2581 cggccgtagc aggccgcgca gacaccgtgg accgcctcgc acgccatcac cgtgcggaca
     2641 tggagttttt ccacgccggc cgcaatgatc tcgccgaaga cgtcgcggta tacgccatcc
     2701 ccaatctcgc tgtggtctcc gacttcctga ccagcatcgg caatcaactc accggtcgcc
     2761 gggtggtaga ccgccgaggc gagcgtgcgg ccgagaacct tggctcggaa gtcgtcgtcg
     2821 ctgagctgct tgccctcggc gtcgaccttc tctatccaga gaccctgcgc cgtgccacag
     2881 tcgccctcgg tcacgatcac gtcctgcgag acatcaacca gtcggcgggt caggtaaccc
     2941 gagtccgccg tccggatcgc ggtgtcggcc agacccttgc gggcgccgtg cgtcgagagg
     3001 aagtactcga gcaccgtcag accttcgcgg aagttggact tgatcggaat gtcgatgatg
     3061 cgaccattcg ggtcaagcac cagaccgcgc atgccggcca tctggctgat ctggttcatg
     3121 tttcccttcg cgccagagtc ggccatcatg tagaccgagt tctgaccctt cagtttcttc
     3181 tcgacgtccg cggccagggt gtcgcgggcc tcgttccaga tcacttccag ctcgcgaagc
     3241 cgctcgttct ctgtgacaag tccgcggcga tactgcttgt caatccgatc agcttcagcc
     3301 tcggccttgt cgacgatctc ggacttcgtc tccgacatct cgacgtcgtc cacggcgatg
     3361 ctcatgccgc cgcgagtcga gtactcgaag ccgaccttct tgatctcgtt gacgatccgc
     3421 gcggtttcga acggatcacc aaacagccgg tagcagtccg caacgacctt gcgcagctgc
     3481 ttgcggtcca tcgtcaggtt gtagaactta ccgatctccg caggcagggc caggttgaag
     3541 atgactcgac cgaccgtagt ctcgagccgc ttgatctcac cgccgtcgcg atcagtgcgg
     3601 acgacaatgc gcgcctgcag atcaaccgcg ccggagtcgt acgcgagctt gacctcttcc
     3661 agcgaggaga agatcttgcc ccagccgcgc ggaaccgtgc cgttcgcgag atcggcatcg
     3721 aagttgcgct cgctggtcat gtagtagcag ccgagaacga tgtcctgcgt cggcgagacg
     3781 atcggatcgc cgttcgacgg agagag
//
LOCUS       DATAOZ010000419         9470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9113622, whole genome shotgun sequence.
ACCESSION   DATAOZ010000419 DATAOZ010000000
VERSION     DATAOZ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9113622"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VHR64_10795"
     CDS             <1..447
                     /locus_tag="VHR64_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303358.1"
                     /translation="DAEEFHLSAQEACVPPTATSTPTSTPTNTPPVIIVPSETPTNTP
                     VVPTNTPVPPTNTPQPPTILVPTNTPTQPSVVVGETVTPAPTETAVPTGTPEKVAGVT
                     SGENMPTSGVGSTFSNGNSVSIFLLLILAAFAGATGWQIRPKERRR"
     gene            complement(527..1048)
                     /locus_tag="VHR64_10800"
     CDS             complement(527..1048)
                     /locus_tag="VHR64_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3303359.1"
                     /translation="MSTDYRVPAQTHIGHVHLKVSDLERSITFYRDVMGFDVVVRYGA
                     QAAFLSAGGYHHHIGLNTWESNGGSGPAPGTTGLYHFAINYPERRDLAAALKRLIDMN
                     WPLDGASDHGTHEALYLSDPDGIGIELAWDRDPSVWTSPDGSIIMKTDRLDLRDLLGE
                     LERTPETATTPGA"
     gene            complement(1103..1462)
                     /locus_tag="VHR64_10805"
     CDS             complement(1103..1462)
                     /locus_tag="VHR64_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303360.1"
                     /translation="MNPHAEEMRRTAFQKSAVLKMPPADALKYSIAYLKERGYRAGPA
                     GRPNQLFVLGGAEGGLPRVTGEIGARADVGKAGTTLLTVDGCGERLGPTLRELVAAIR
                     AESKARTAAASGSDATG"
     gene            complement(1470..2156)
                     /locus_tag="VHR64_10810"
     CDS             complement(1470..2156)
                     /locus_tag="VHR64_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX3303361.1"
                     /translation="MKITFIGHSAFALEADGKQVLVDPFITGNPVANVSPGDFSPQTI
                     ILTHAHNDHVGDTVDIAKRSGAKVIATAELANWVGSQGVEGATGANHGGTVRFSGGST
                     KFVPAWHTSSYSTKDGVVAPGVPAGHIVRFGGKTLYFAGDTCLFGDMALIGEAGIDVA
                     IIPIGDHFTMGPDDALRAVQLISPAMVIPCHYNTFPPIQQDGMAFKKRVETDTTARCL
                     LLNPGESSEI"
     gene            complement(2175..3779)
                     /locus_tag="VHR64_10815"
     CDS             complement(2175..3779)
                     /locus_tag="VHR64_10815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3303362.1"
                     /translation="MSADVVLINGHVMTVDPINPVASAVAIRAGRVVHVGSDADARAA
                     AGPRAEVIDLRGRTATPGLNDAHAHPMAVGQALLDVDISPERVGSIEGIGRAIKGRLN
                     GSEPGTWIIGRGYDQARMPDQRHPTRADLDTVAPNNPVFLYRACHHIAAVNSAALKLA
                     GVTARTRDPEGGTIDRDEHGEPTGILREAAMGLVHDHIPAPTEDSIMRALEMAGRAFL
                     RTGVTSTVEAGIRLPREMSAYQRLRQSDTLPVRTYLMMMIDETLDHMASLGIKSGFGD
                     SWLRIGPAKLFLDGSIGGRTCRMRQPFEGEAENVGLWMEPPDVMKSKVIKAHSLGFQV
                     GVHAIGDAAIDLILDAYEEAQAKYPRQDTRHRIEHCSIVDFETIQRIKRLGAVPIPGT
                     SFLYYFTPAYLQNIGAQRLRYGVCMRTFAEQGIVAAASTDAPVVPVSALTGLGMMMSR
                     TDVNGDEIWTEETVDLETAIRAYTWNGAYASFEEHSKGSLENGKLGDVCVFETDLRQV
                     APKEIKDVAVDLTIAEGRVAYDRSVG"
     gene            complement(3802..4632)
                     /locus_tag="VHR64_10820"
     CDS             complement(3802..4632)
                     /locus_tag="VHR64_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3303363.1"
                     /translation="MMVLPQAEDGYAPNRWPGGHRCALCLSFDVDGPYGEANYQPASN
                     TYAISQTEYDPFGTRRILEILADADVPATFCWVGKEAQDRPELVHRAAGQGHEIALHS
                     WDHRYYHEMSADEQREDMLRTSDALFAITGHRPVGHKTAGWRYTADTHRLCQEIGLIW
                     AMDIPRGDLPFLDRPDPARDPLVQLPPAAQWDDYNFFVDKMLTPNQAYEFWRDDLDVI
                     RAEGSLMSLTLHPFVSGRPAPSRALVRLIDYAIDLGDVWLARADQIAEWWLSQHQSGQ
                     "
     gene            complement(4642..5862)
                     /locus_tag="VHR64_10825"
     CDS             complement(4642..5862)
                     /locus_tag="VHR64_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3303364.1"
                     /translation="MSTTQTSGPQISTNGASPDREKLDALRSQLPAVTCTRYFNAGSN
                     GPIPRPAHDVLLNQATEDLTMGRCDLAFYGKRKDESVHLRETIAGIFNANVSEVAIMR
                     STTEGMNAALNGVLWRAGDEIITTHLEHICLFSAIGSVSHRHGVTVRTVNIGDGGGDV
                     LGAIASAVTPRTRVIAVSHVHWTTGAVMPLAEIAEFARERGILTIIDGAQGAGQVEVD
                     FHALGVDAYCQAGQKWLCGPNSSGFMLLRDDRLGDFRTSYLRYGTFDPAGYVVPPPGA
                     ARFEMGETYGPAIAAFDRGLEWLRDEVGFDWLIARNAELGKRCLNALKEVEGITIGTP
                     EDKMAGMVCFNIPGMHPKDVSDRLAERGQTIRFVDQRPSEPTARISTAWWTTEEEVDE
                     LVAEIAELAKSVQS"
     gene            complement(5989..7083)
                     /locus_tag="VHR64_10830"
     CDS             complement(5989..7083)
                     /locus_tag="VHR64_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX3303365.1"
                     /translation="MPDSITARGVIAYKPGEPGVVEDFLVPHPGPGEALVRILASGVC
                     HTDLSAKNGVFGTDYFPFLLGHEGSGIVEEIGEGVTNVSRGDFVILAWRAPCGECRFC
                     LVGKPHLCAASLNATQKMTTMDGTVLTPILGIGTFCTHTLVHTKQCIKVSKDLPPEQM
                     SLIGCGVMTGVGAALYTANVQPGQSVAVVGCGGVGDSVIMGAKLAGATTIIAVDVDPK
                     KLEWAKGFGATHTVNPKDGDPVAAIKEITSGCGVNASFEAVGKPETLMTALWARDLAG
                     TCVIIGVAGQEAIVEFPFAKFFDLGGALKVSWYGDCLPTRDFPLLADWYSQGQLDLDG
                     VVTRVISLEDSEEAFHAMERGETLRSVIKL"
     gene            7242..8195
                     /locus_tag="VHR64_10835"
     CDS             7242..8195
                     /locus_tag="VHR64_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX3303366.1"
                     /translation="MTGDANQDRFLGAILGMAIGDAFGMPVSGWSATTIADWYGAVRD
                     YLPRTFSDGEEVSPGEISDETEIALCIIESVTAAQGEIDVENIGVRMSYLARSPSRRW
                     LSDETATALDGRSEEHEYQLPLVDDEQVSADILARGIPIGLMHSMGAFNEAALRADAS
                     LVARITHGSPLALTLVEAVARAVALSARGATQLDQLPLLIAEDLPDGQVRVALTEPEI
                     GERSEPNRVLTSALDIIGAASSFQDALERAVALGGSTDARSTLVGALYSGHHGSATIP
                     QRLIDGLEARIYVSLAVPWFYRTVARLRGRAIDLRADFGPV"
     gene            8304..9428
                     /gene="dnaJ"
                     /locus_tag="VHR64_10840"
     CDS             8304..9428
                     /gene="dnaJ"
                     /locus_tag="VHR64_10840"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX3303367.1"
                     /translation="MTDKRDYYEVLGVSRTASADELKKAYRKLARQYHPDVNRGDASA
                     EERFKEVNEAYEVLSDADRRAAYDRFGHAANGMGGDPFGGFGGSPFGDLFESFFGGGN
                     SRRRTGPARGQDLTISVSLTFEEAVFGVDKEVEVLRLETCESCHGTRMKDGQTPPRCA
                     TCGGTGEIRRVQQTILGQFMTSNPCPACRGEGVTITDPCPTCRGRGRTSQARKITVTV
                     PAGIDENQTLRLSGQGEAGPSGGPAGNLFINFSIKPHALFSRQGKQVISAVGINVAQA
                     MLGDEITVPTLDGDVSLKVPSGTQSGQQFRLRHKGVPDMRGGDRGDQIVTIHVNIPDT
                     LTEKQRELITELAESFGREVSPQPAASRGFFDKVKDALGV"
BASE COUNT         1757 a         2916 c         2980 g         1817 t
ORIGIN      
        1 gacgccgagg aattccactt gagcgctcag gaggcctgtg ttccgccgac cgcgacgagc
       61 acgccaacca gcactccgac taatactccg ccggtgatta tcgtcccgtc ggaaacgccg
      121 accaatactc cggtagtgcc gacgaacacc ccggtgccgc cgaccaacac gccccagccg
      181 ccgacgattc tggtcccgac aaatacgccg acgcagccgt cggtcgtggt tggcgagacg
      241 gtgaccccgg ccccgacgga aactgccgtt ccgacgggca caccggaaaa ggtggccgga
      301 gtcacgagtg gtgaaaacat gccgacaagc ggtgttggtt ccaccttctc gaacggcaac
      361 tcggtctcga tcttcctgct cctgatactc gcggccttcg ccggagccac gggctggcag
      421 attcgaccga aggagcgtcg ccgctaacga gcgatagcgc gagtcaaaag aacgccggcc
      481 ccgtgttccg gggccggcgt ttttcgtgtt gatgccttgc ggtctgctat gccccgggcg
      541 tcgttgcggt ctccggagtt cgctccagtt ctccgaggag atcgcgcaag tcaagtcgat
      601 cggtcttcat gattatcgac ccgtctggtg aggtccaaac actggggtca cggtcccaag
      661 ccagctcgat gccgatgccg tccgggtcgc tcaaataaag cgcctcatgg gtaccgtgat
      721 cggatgcgcc atcgagcggc cagttcatgt cgatcaatcg cttgagagcg gccgcaagat
      781 cccggcgctc cgggtagttg atcgcgaagt gatagaggcc ggtcgttccc ggcgctgggc
      841 ccgacccccc gttactctcc cacgtgttca gaccaatgtg atggtgatag ccaccggctg
      901 acaggaatgc cgcctgggcg ccgtatcgaa cgacgacgtc gaagcccatg acatcccgat
      961 agaacgtgat tgagcgctcg aggtcggaga ccttgagatg gacatgcccg atgtgggttt
     1021 gggcgggtac gcggtaatca gtggacatat tgacctctca ctaacgaatc gtaagtaact
     1081 tcatcataca tgaggtgtca actcagcctg tcgcgtcgct ccctgatgct gcagccgtgc
     1141 gggccttgct ttcggcacga atggccgcaa ccagctcccg gagcgttggt ccaagacgct
     1201 ctccgcagcc atcgacggtc aggagcgtgg tgcctgcctt accaacgtcg gcccgggctc
     1261 caatctcacc ggtcacgcgg ggcagtccac cctccgcacc gccgagcacg aagagctggt
     1321 ttgggcgacc ggcgggtccg gcgcgatatc cccgctcctt caggtatgcg atcgagtatt
     1381 tgagggcatc ggccgggggc atcttgagga cagcgctctt ctgaaacgcg gtccggcgca
     1441 tttcctcggc gtgcggattc atgtccgaat cagatctcgc tgctttcgcc cgggttgagc
     1501 agcaggcacc gggccgtcgt gtcggtttcg acacgcttct tgaatgccat cccatcctgt
     1561 tggatcggcg ggaaggtgtt gtagtgacag gggatgacca tcgccgggct gatgagctgc
     1621 acggctctca acgcatcatc cggccccatc gtgaagtggt cgccgatcgg gatgatcgca
     1681 acgtcgatgc cagcttcacc aatcagcgcc atgtcgccga agagacaggt gtcgccggca
     1741 aagtagagcg tcttgccgcc gaatcgaacg atgtgtccgg cggggacacc cggcgccacg
     1801 actccatcct tcgtggaata cgacgaggtg tgccacgccg ggacgaactt ggttgagccg
     1861 ccgctgaacc ggaccgtgcc gccatggttc gcgccagttg caccctccac accctgggag
     1921 ccgacccagt tcgcgagctc ggcggtcgcg attactttgg cgcctgatcg tttggcgatg
     1981 tcgaccgtgt cgccgacgtg gtcgttgtgg gcatgtgtca ggatgatcgt ttgcggcgag
     2041 aaatcgcccg gggagacgtt cgcgaccgga tttcccgtga tgaacggatc aaccaggacc
     2101 tgtttgccat ccgcctcgag cgcaaatgct gagtgcccaa taaacgtgat cttcatggcg
     2161 cctcctgtcg tttgctatcc caccgagcga tcgtaggcga cccggccctc cgcgatggtc
     2221 aggtcgacag caacgtcctt gatctccttc ggagcgacct ggcgcaaatc tgtctcgaat
     2281 acgcagacat cgccgagttt cccgttttcc agcgaaccct tgctgtgctc ctcgaaggag
     2341 gcgtacgcgc cgttccaggt gtaggcgcgg atggcggtct cgagatcgac agtctcctcg
     2401 gtccagattt catcgccgtt cacgtctgtt cggctcatca tcatgccgag gccggtcaag
     2461 gcggacaccg gaacgaccgg cgcatcggtg ctggccgccg cgacaattcc ctgctccgcg
     2521 aacgtccgca tgcagacgcc ataccgcagg cgctgtgcgc cgatgttctg gaggtaggcg
     2581 ggcgtgaagt aatagaggaa agaggtgccg ggaatcggga ctgccccaag ccgcttgatc
     2641 cgttggatcg tctcgaagtc gacgatcgag cagtgctcga tccggtgccg cgtgtcctgc
     2701 cgcgggtatt tggcttgcgc ctcttcgtaa gcatcaagga tgaggtcaat cgccgcgtcg
     2761 ccgatcgcgt gaacgccgac ctggaaaccg agcgagtgcg ccttgatgac cttggacttc
     2821 atcacatctg gcggttccat ccagagtccg acattctctg cctctccttc gaacggttgc
     2881 cgcatccggc aggtgcgccc gccgatcgag ccgtcgagga agagcttggc cgggccgatc
     2941 cgaagccagg agtcgccaaa ccccgacttg atccccaggc tggccatatg atccagggtc
     3001 tcgtcgatca tcatcatgag ataggtgcgg accggtagcg tgtcgctttg gcgaaggcgc
     3061 tggtaggcgg acatctccct gggaaggcga attccggctt cgaccgtgct ggtcacgccg
     3121 gtgcgcagaa atgcccgccc ggccatttcc agcgcgcgca tgatcgagtc ttcagtcggc
     3181 gcgggaatgt ggtcgtgcac cagccccatc gccgcctctc gcaagatacc ggtcggctcg
     3241 ccatgctcat cccggtcgat cgtgccgccc tctggatccc gtgtgcgtgc ggtcacgccc
     3301 gcgagcttaa gggccgccga gttgaccgcc gcgatgtggt ggcaggcccg gtacaggaag
     3361 accggattgt tgggcgcgac ggtatcgaga tcggcgcgcg tcggatggcg ctgatcgggc
     3421 atccgagcct ggtcataacc gcgaccaata atccatgtgc ccggctccga gccattcagc
     3481 cggcccttga tggcgcggcc aatcccctcg atcgagccga cgcgctccgg cgaaatgtcg
     3541 acgtcgagca gcgcttgtcc aacggccatc gggtgggcgt gggcatcatt cagccccggc
     3601 gtcgcggttc ggcctcggag gtcgatcacc tcggcccggg ggccggcagc cgcgcgggcg
     3661 tccgcgtccg atccgacgtg gactacgcga ccggcgcgaa tagccaccgc actggcgacc
     3721 gggttgattg gatcgaccgt catgacatgg ccgttgatca ggacgacatc agcagacatt
     3781 gtttccccct gagcccggca gctattggcc agactgatgt tgtgagagcc accactctgc
     3841 gatctgatcg gctcgggcca accagacatc acccagatca atggcgtagt cgataaggcg
     3901 aaccagcgcg cgagagggcg cgggtcgtcc ggaaacgaac ggatgcaggg tcagactcat
     3961 aagcgagcct tcggcccgga tgacatcgag gtcatcgcgc cagaactcgt atgcctgatt
     4021 cggtgtcagc atcttgtcga cgaagaagtt gtaatcgtcc cattgcgcgg ccggtggcaa
     4081 ttggacaagt gggtctcgtg cgggatccgg tcggtcgagg aatggcagat cgccgcgcgg
     4141 gatgtccatc gcccagatca ggccgatctc ctggcagagc cggtgcgtgt ccgccgtgta
     4201 ccgccaacca gccgttttgt gcccgacagg ccggtgtccg gtaatcgcaa acagcgcatc
     4261 cgaggtgcgg agcatgtctt cgcgctgctc atcagccgac atctcatggt agtaccggtg
     4321 gtcccacgag tgcagcgcaa tctcgtgccc ctgcccggca gcacgatgga cgagctcagg
     4381 gcggtcttgc gcttccttgc cgacccagca gaaggtggcc ggcacatcgg cgtcggcgag
     4441 gatttccagg attcgtctgg tcccgaacgg atcgtattcc gtctgagaga tagcgtaggt
     4501 attcgacgcc ggttggtaat ttgcctcacc gtacggcccg tcgacgtcga agctcaggca
     4561 aagagcgcac cggtggccac cgggccaccg gttgggcgcg tagccgtcct ctgcctgcgg
     4621 taaaaccatc atgggtgctc gctacgactg aacagacttc gcaagctcgg caatctcagc
     4681 gaccagttcg tcgacttctt cctcagtcgt ccaccatgcg gtagagatgc gagcggttgg
     4741 ctcgcttggt cgctgatcga cgaagcggat cgtctgcccg cgctccgcga gccgatcgga
     4801 gacatccttg ggatgcatgc ccgggatgtt gaagcagacc atgccagcca tcttgtcctc
     4861 gggtgtgccg atcgtgatgc cctcgacctc tttcagagcg ttcaggcagc gcttgccaag
     4921 ttctgcgttg cgggcgatca accagtcgaa cccgacctca tcgcgcagcc actcgaggcc
     4981 gcgatcaaat gcagcaattg ccggaccata ggtctcgccc atctcgaagc gcgcggcgcc
     5041 cggcggcggg acgacgtagc cggccggatc gaatgtgccg taccgcaaat aactggtgcg
     5101 gaaatcaccg agccggtcat cgcggagcaa catgaagcca gagctgttcg ggccacagag
     5161 ccacttttga ccagcctggc aataggcgtc gacaccaagt gcgtggaagt cgacttcgac
     5221 ctggcccgcg ccttgggcgc catcgatgat tgtcagaatc ccacgctccc gcgcgaactc
     5281 tgcgatctcg gcaagcggca tgaccgcgcc ggtggtccaa tgcacatggg agacggcgat
     5341 gactcgcgtc cgaggagtga cagccgacgc gatcgcgccg agtacgtcgc caccaccgtc
     5401 accgatgttc acggtccgca ccgtaactcc gtgtcggtgc gagacgctgc cgatcgctga
     5461 aaagaggcag atgtgttcga ggtgcgtggt gatgatctcg tcacccgctc gccaaagcac
     5521 gccgttgagt gcggcgttca tgccctcggt tgtgctccgc atgatcgcga cttccgagac
     5581 attggcgttg aagatgccgg caatcgtttc gcgcaggtgc accgattcat ctttgcgctt
     5641 gccataaaag gcgaggtcgc atcggcccat cgtcagatct tcggtggcct ggttgagcag
     5701 gacgtcgtgt gccggccgag ggatcggccc gttcgagccg gcattgaagt accgagtgca
     5761 cgtaaccgcg gggagttggc ttcggagggc atcgagcttc tcgcgatcgg gagaggctcc
     5821 attggtgctg atctggggtc cggacgtctg cgtggttgac aacgaggttc tctccgtcac
     5881 gaaaacgaga catatggtcg gcagtctatg accgctggcg cgctcggtca aacgggctgg
     5941 aacgcaatga atttcccagc cgtggatcgt ggcgcgccgt tgggtttctt acagtttgat
     6001 cacgcttcgc aacgtctcac cgcgttccat tgcatggaat gcctcctcgg aatcttcgag
     6061 gctaatcacg cgcgtcacta cgccgtccag atcgagctga ccctgtgagt accagtcggc
     6121 gaggagcggg aagtcgcgag tcggcaagca gtctccgtac catgagacct tgagtgcccc
     6181 gccgagatcg aagaacttcg cgaacggaaa ctcgacgatc gcctcttgcc cggccacacc
     6241 gataatcacg cacgtcccgg ccaggtcccg tgcccagagt gcggtcatca gcgtttccgg
     6301 cttgccgact gcttcgaagg aggcattcac tccacacccg ctggtgattt ccttgatcgc
     6361 cgcgacggga tcaccgtcct tcgggttgac cgtgtgggtt gcaccgaatc cctttgccca
     6421 ttcgagtttc ttgggatcga cgtcgaccgc aatgatcgtg gttgcgccgg caagctttgc
     6481 gcccatgatc accgagtcac ccacacctcc gcagccgacc acggcgacgg attgtccggg
     6541 ctgaacgttg gccgtataaa gcgcggctcc gacgccggtc atcacgccgc atccaatcag
     6601 ggacatctgc tcgggcggga gatccttgct gaccttgatg cactgtttgg tgtgcacgag
     6661 cgtgtgcgtg cagaacgtgc caatgccgag gatcggggtc agcaccgtgc catccatcgt
     6721 tgtcattttc tgggtggcgt tcaggctggc ggcgcagagg tgcggcttgc cgacgaggca
     6781 gaaccggcac tcgccgcagg gggctcgcca ggccagtatg acgaagtcgc ccctggacac
     6841 attggtgacg ccttctccga tttcttcgac gatgccggac ccttcgtgcc cgagcaggaa
     6901 cgggaaataa tcggtgccga atacgccatt ttttgcggag agatccgtgt ggcagacgcc
     6961 gctcgcgagt attcgcacca gcgcctcgcc tggtcctgga tgagggacaa ggaagtcctc
     7021 aacgacaccg ggctcgccgg gcttgtaggc gatgacgccg cgcgcagtaa tcgaatcggg
     7081 catgctcaga acctccttgt tctagagtat ccatctgata gtgggcatcc tctgtccaga
     7141 cttcggtcgc tgtcaagcga ccgtttcgaa aataaccagc gccgcattcc aagcgtacaa
     7201 ggctcgtgga tgcacccgag gctctcgaac aggataggca attgaccggc gacgcgaatc
     7261 aggaccgatt tcttggcgca atactgggaa tggcgatcgg tgatgcattt ggcatgcccg
     7321 tctctggttg gagcgcgacg actattgctg actggtacgg ggctgtcagg gactacttgc
     7381 cgcgcacgtt ttcggatggc gaggaagtca gtccaggtga gatctccgac gagacggaga
     7441 ttgccctttg cattatcgaa tcagtgaccg ctgcccaggg cgagatcgac gtcgagaaca
     7501 tcggcgtgcg gatgtcctac ctggcacgct cgccatcgcg ccgctggctg agcgacgaga
     7561 cggccacagc actcgacggc cgatcggagg agcacgagta tcaacttcct ctcgtcgatg
     7621 acgagcaggt gagcgcggac atcctggcga gaggaattcc gatcgggctg atgcacagta
     7681 tgggcgcgtt caacgaggcg gcactccggg cagacgcctc gctggtcgct cgaatcacgc
     7741 atgggagccc gctggctctg acgctggtcg aagcggtcgc gagagcggtc gcgctatcag
     7801 ctcgcggggc aacgcaactg gaccaacttc ctctgttgat tgcggaggat ctgccggacg
     7861 ggcaggtccg ggttgcgtta accgagcctg agattgggga gcgatcggaa ccgaaccggg
     7921 tgctgacaag cgcgctcgac atcattgggg ccgcctcgtc gtttcaggac gcacttgagc
     7981 gcgcagtggc gttgggtggg tcgacggacg cgcgctcaac ccttgtcggc gctctgtaca
     8041 gcggtcatca cggaagcgcg acgataccgc aacggctcat cgacggcctc gaggctcgaa
     8101 tctatgtgtc gctggcagtg ccgtggttct atcgaaccgt tgccagattg cggggccgcg
     8161 cgattgatct ccgggctgac tttggcccgg tttgattgac ggagtggtag ccctcactat
     8221 actttgggaa gaacgcgccc tgaagccggg gcgcgtttca tgagcctgat ctgtaatttc
     8281 tcccgcacta catcatgaag acaatgaccg acaaacgcga ttattacgaa gtacttgggg
     8341 tcagccgaac ggcctcagcc gatgaactaa agaaggcgta tcgaaagctc gcccggcagt
     8401 atcacccgga cgtgaaccgg ggcgacgcgt cggccgaaga gcgattcaag gaagtcaacg
     8461 aagcgtacga agtactctcc gacgccgatc gccgggctgc ctacgaccgg tttggtcatg
     8521 cggcgaacgg catgggcgga gatccgttcg gcggtttcgg tggatcgccg ttcggcgacc
     8581 tgttcgagag cttctttggc ggcgggaact cccggcgccg gacaggtcca gcgcgtgggc
     8641 aggacctcac gatttccgtt tccctgacat tcgaggaagc ggtcttcggc gtcgacaagg
     8701 aggttgaggt tctccgcctc gaaacttgcg agtcgtgcca tggcacacgc atgaaggacg
     8761 gccagacacc gccacgttgt gccacgtgtg gagggacagg cgagatccgt cgcgtccaac
     8821 agaccatcct cggtcagttc atgaccagca atccctgtcc ggcgtgtcgt ggcgaaggcg
     8881 ttacgatcac cgatccttgc ccgacctgcc ggggacgggg ccgcacgtcg caagcgcgga
     8941 agattaccgt caccgttccg gcgggcatcg acgagaacca gacgctgcgc ctgtccggtc
     9001 agggcgaggc gggaccgtcg ggaggtccgg ccggcaacct gttcatcaac ttcagcatca
     9061 agccccacgc gctattcagc cggcagggca agcaagtcat ctcggcggtt ggcatcaatg
     9121 ttgcgcaggc gatgctgggc gacgagatca cggtgccgac ccttgacggt gacgtttcac
     9181 tcaaggtccc gtctggtacc caatcgggcc aacaattccg cttgcgccac aaaggtgttc
     9241 cggacatgcg cggcggggac cgtggcgatc agatagtgac cattcacgtc aacatccccg
     9301 atacgttgac cgaaaagcag cgagagttga tcaccgaact cgcagagagc ttcggccgcg
     9361 aggtgagccc gcaacccgcc gcatcgcgcg gcttcttcga caaagtcaag gacgccttgg
     9421 gcgtttagct cgcggtaaca ctctcgggct gcgccgtgcc aattccggct
//
LOCUS       DATAOZ010000420         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3065393, whole genome shotgun sequence.
ACCESSION   DATAOZ010000420 DATAOZ010000000
VERSION     DATAOZ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3065393"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1855
                     /locus_tag="VHR64_10845"
     CDS             <1..>1855
                     /locus_tag="VHR64_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX3303368.1"
                     /translation="PNAGYVLELYERYTQDPASVSPADKAYFDRLDPAALVALTAAPS
                     RISTNGVAAPAADVAKITAATALAQAVREYGHLDATIDPLGSTPPRAPELDPGFHGLT
                     EADLAHLPATVVSGPAANDSSNAAAALQKLREIYSGSVGYDFDHIQVAKERLWLHDAV
                     ESRRFSAPLESATRRALLRRLTQVESFERFLHQTYLGQKRFSVEGNDMVVPMIDECVH
                     AAAASGTREVVIGMAHRGRLNVLTHILGKPYAAIIQAFEGKKKSKAVGGDPSNVNDPS
                     EDFSGDVKYHLGARLLRGEHGMAVEVPMVLAPNPSHLEQVNPVIEGMVRASQDITDRP
                     GEPGLDPKASVAIAIHGDAAFPGQGVVAETLNMAGLRGYTTGGTIHIIVNNQVGFTTD
                     PIDSRSTLYASDLAKGFEIPVVHVNADDPEACLAVCRMAMAYRQEFGKDFLVDLVGYR
                     RWGHNEGDEPMFTQPVLYEKITNHPTVRAIWADKMIADGDITQADADQLVSEAMDRLA
                     GVRRSVTDGTAEVEEHEHHPAGRREVETALPEARLREIHQAIHALPEGFAITSKLARQ
                     WDRRAKLLDSPDAKVDWALAETYAFAATISDRIPVRLSGQDAERGTFSQRHL"
BASE COUNT          325 a          656 c          575 g          299 t
ORIGIN      
        1 ggccgaatgc cggctacgtg ctggagctct acgagcgcta tacccaggac ccggcatcag
       61 tttcccccgc cgacaaggcg tacttcgacc gcctcgatcc ggccgcgctg gttgccctca
      121 ctgcggcacc cagccggatc agtacgaacg gcgtcgctgc gccggccgcg gacgtcgcga
      181 agatcacggc ggccaccgcg ctcgcccagg cagttcgcga gtacgggcac ctggatgcga
      241 cgatcgatcc acttggctct accccgccgc gtgcccctga gctcgatccc ggcttccacg
      301 gtctgactga agccgatctg gctcatctgc cggcgacggt cgtgagcggg ccggccgcga
      361 atgactcgtc taatgcggcg gccgcgctgc agaagctgcg cgagatctac tcaggctcgg
      421 tcggctacga cttcgaccat atccaggtcg caaaggagcg actctggctg cacgacgccg
      481 tcgagtcccg ccgattcagc gcgccgttgg agtcggccac ccgtcgcgca ctcttgcgcc
      541 gcctgaccca ggtcgagagc ttcgagcgct tcctgcacca gacctatctc ggccagaagc
      601 gcttctcggt cgaaggcaac gacatggtcg tgccaatgat cgacgagtgc gttcatgccg
      661 ccgcggcctc cggcacgcgc gaagtcgtga tcggtatggc ccaccgtggt cgcctcaacg
      721 tgctgaccca catcctcggc aagccctacg ccgcgatcat ccaggccttc gagggcaaga
      781 agaagtcgaa ggcggtcggc ggcgacccat ccaatgtcaa tgatccgtct gaagatttct
      841 cgggcgatgt caagtaccac cttggcgccc ggctcctgcg cggcgagcat gggatggcgg
      901 tcgaagtgcc gatggtcctc gcgccgaacc cgagccacct cgagcaggtc aacccggtca
      961 ttgagggcat ggtccgcgcc tcgcaagaca tcaccgatcg ccccggcgag ccgggactcg
     1021 accccaaggc cagcgtcgcg atcgcgattc acggcgatgc cgctttcccg ggccagggtg
     1081 tcgttgccga gaccctgaac atggccggcc tccgtggcta tacgaccggc ggcacgatcc
     1141 acatcatcgt caacaaccag gttggcttca cgactgaccc gatcgactcc cgctcgaccc
     1201 tctacgccag cgacctcgcc aagggattcg agattccggt cgtccatgtc aatgccgatg
     1261 atcccgaggc ctgcctcgcg gtctgccgga tggcgatggc ctaccgccag gagttcggca
     1321 aggacttcct ggtcgatctg gtcggctacc ggcgctgggg ccacaacgaa ggcgacgagc
     1381 cgatgttcac ccagccggtc ctttacgaga agatcacaaa tcacccgacg gtgcgcgcaa
     1441 tctgggccga caagatgatc gccgacgggg acatcacgca ggcggatgcc gatcaactcg
     1501 tctccgaagc gatggatcgc ctggccggcg tacgccgctc ggtcaccgac ggtacggccg
     1561 aggttgagga gcacgagcat cacccggctg gccggcgtga ggtcgagacg gccctcccgg
     1621 aagcccggct gcgtgagatt caccaagcca tccacgccct gcccgagggc ttcgcgatca
     1681 catcgaagct cgcccgccag tgggaccgtc gtgccaagct gctcgactcc cctgacgcga
     1741 aggtcgattg ggcgctcgcc gagacttatg ccttcgccgc gacgatctcg gaccggatcc
     1801 cggtccgcct gagcgggcag gatgccgagc gcggcacctt cagccagcgc cacct
//
LOCUS       DATAOZ010000421         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_957719, whole genome shotgun sequence.
ACCESSION   DATAOZ010000421 DATAOZ010000000
VERSION     DATAOZ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957719"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VHR64_10850"
     CDS             <1..821
                     /locus_tag="VHR64_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303369.1"
                     /translation="IDLSVASILGLTSAVMGEAWMNDWPLWACILLALAVGATCGLFN
                     GLLVTWMRLPSLVVTLGTLALYRGLAYVVLGSDAVSDYPQKFNDFGFDAITGTSIPWS
                     ALIFVALLGIFLVLLHRSRAGRQIYAVGLNQEAARYSAVRVARLKLWLFVLSGTIASV
                     AGILFTARVSSSRADNAVGFELDVIAAVLLGGVSIFGGKGTLVGVALSLAVVATIRNA
                     LGITNVGPELQSIVIGSLLILSVLGPNLVGRLTHVGRRSIVGLRGLSPGPSTGD"
     gene            1103..2248
                     /gene="rhaS"
                     /locus_tag="VHR64_10855"
     CDS             1103..2248
                     /gene="rhaS"
                     /locus_tag="VHR64_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3303370.1"
                     /translation="MKKQVPMLRLLMVFMFAMLGFSTGIHAAAPAMAQDANTTCAGEP
                     MIGAPTASPTAGSPVAGISASPAATIDPATLNIAFLPKDVVNPYFAASAAGAQAAATE
                     LGGQFQQVGPQTSNAAEQVTWIQTLTQQKVAAIVVSANDPDALAPALKDAAAQGIKVI
                     SFDSDVAPDARIAFANQADSDQIGRIQVQIMGKELNCEGDIAILSAAATATNQNAWID
                     VMKDELAKPGYENLKLVEVAYGDDDPQKSHDKTIELLTAYPDLKGIISPTTVGITAAA
                     QALEEEGRGGGSDNQVKLTGLNLPNQMKPYVTSGTVEEFALWDPGNLGYLAYYMAANA
                     IAGNLTGAEGETFTAGKLGTYTVGPNGTVILGPPTIFNADNIDNYNF"
BASE COUNT          434 a          737 c          691 g          430 t
ORIGIN      
        1 agatcgatct ctcggttgcc tcgattttgg gcctgaccag cgcggtcatg ggcgaagcct
       61 ggatgaacga ctggccgctc tgggcgtgca tccttctggc gctcgcggtc ggcgcgactt
      121 gcggcctctt caacggcttg ctggtgacct ggatgcgact gccgtcgctc gtcgtcacgc
      181 tcggcacact cgcgctctat cgcggtttgg cgtacgtcgt gctcggttcc gacgcggtca
      241 gcgactaccc gcagaaattc aacgactttg gcttcgacgc gatcaccggc acgagcatcc
      301 cgtggtcggc gctgatcttt gtcgccctcc ttggcatctt cctggttttg ctccatcgca
      361 gccgggcggg tcgccagatc tacgcggttg gtctcaatca agaggctgcc cgctactcgg
      421 ccgttcgcgt ggcgcggctc aagctctggc tctttgtcct ctcgggaacc atcgcgtccg
      481 tcgccggcat cctctttacc gcccgtgtct ccagcagccg cgcggacaat gcggttggtt
      541 tcgagctgga cgtaatcgcg gccgttttgc tcggtggtgt cagcatcttc ggcgggaagg
      601 gaacgctggt tggcgtcgcg ctctcgcttg cggtcgtcgc cacgatccgc aacgccctcg
      661 gcatcaccaa tgtcggcccc gagctgcaaa gcatcgtcat cggcagcctg ctgatcttat
      721 cggttcttgg tcccaatctc gtcggacgcc tgacccatgt cgggcgacgt tccatcgtgg
      781 gactgcgggg tctcagcccc ggaccaagca caggtgatta gtgttcacgg aaaggagggg
      841 aagggatatt caattcgaac cagttcgatg gattttcaca tcgttgctca atcccgggaa
      901 tgaattcccg ggctcagaga ccgaagtccc tccgggacat gcaatggctg cctcgacttt
      961 tcgatacgtc ggcttcagcc gacatccggg aatgagcccg ggaatcaatt cccgggcggg
     1021 gcactgagac tgcaagatcc gaatccatca acgacgctct gctactgcgc ggagcgtgaa
     1081 gtggaagaaa ctggaggaca cggtgaagaa acaagtccca atgctccggt tgctgatggt
     1141 cttcatgttt gccatgctcg gtttcagcac aggaattcac gccgcggctc cggcgatggc
     1201 ccaggatgcg aacacgacct gcgccggcga accgatgatc ggcgcgccga ccgccagccc
     1261 gacggctgga agtccagtcg cggggatctc ggccagtccc gccgcgacga tcgatcccgc
     1321 cacgctcaat atcgccttcc tgccgaagga cgtggtcaac ccctacttcg ccgcgtctgc
     1381 cgccggcgcc caggcagcgg cgaccgagct cggtggccag ttccagcagg tcggaccgca
     1441 gacctcaaat gcggccgagc aagtcacctg gattcagacg ctgacccagc agaaggtcgc
     1501 ggcgatcgtc gtctccgcga acgaccccga cgcactcgcc ccggcgctga aggatgcggc
     1561 cgcgcagggg atcaaggtca tttccttcga ctctgatgtc gcaccggacg cccgcattgc
     1621 ctttgccaac caggccgatt ccgaccagat cgggcggatt caggtgcaga tcatgggcaa
     1681 agagctcaac tgcgaaggtg acatcgcgat tctttccgcg gcagcgactg ccacgaacca
     1741 gaatgcctgg atcgacgtca tgaaggatga gctcgcgaag ccgggctacg aaaacctgaa
     1801 gctagtcgag gttgcctacg gcgacgacga cccgcagaag agccatgata agaccatcga
     1861 gctcctgacc gcctatcccg atctcaaggg catcatctcc ccaacgaccg tcggcatcac
     1921 cgcggcggcg caggcgctcg aagaagaagg acgcggcggc ggatcggaca accaggtcaa
     1981 gctgaccggt ctcaacctcc cgaaccagat gaaaccatac gtcacgagcg gcacggtgga
     2041 agagttcgcc ctgtgggatc cgggcaatct tgggtacctg gcctactaca tggcggcgaa
     2101 cgcgattgcc ggcaacctga cgggcgcaga aggtgaaaca ttcaccgctg gcaagctcgg
     2161 cacgtacacg gtcggaccga acggcacggt cattctcggc ccgccgacga tcttcaacgc
     2221 cgacaacatc gacaactaca acttctagtc gctggatgtc gcggctgtag ttaccgcggc
     2281 gaccgacggc ct
//
LOCUS       DATAOZ010000422         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9114841, whole genome shotgun sequence.
ACCESSION   DATAOZ010000422 DATAOZ010000000
VERSION     DATAOZ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9114841"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..1260
                     /locus_tag="VHR64_10860"
     CDS             496..1260
                     /locus_tag="VHR64_10860"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HEX3303371.1"
                     /translation="MTVSKASDITAKLLEPFPVDVIEEKNGLLYIPHEIIRDRLISAT
                     DNQFDWSIDQILFRDDGVTRRANDRYTGEPKRPLSMIVIGTLTIPGLGSRAGIGAHPL
                     DEGAGEDAAYKSAESDAVKRAAMAFGVGLHQLYIETGEAKRVQKPQRNGGQVRAMARP
                     APQQRSEPRQSELKPANDKQFGNEVRQAMASRDATAFRKLVDDANDHVGRWVALVQAA
                     ESPQALDWIKRQIERKGVESDILTHEIRKREEQIAA"
     gene            1861..>2710
                     /locus_tag="VHR64_10865"
     CDS             1861..>2710
                     /locus_tag="VHR64_10865"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX3303372.1"
                     /translation="MTITLSPLEIWLHAIRPKTLFASVSPIIVGSAVAYHEDGFHLPT
                     ALIALAVAVLLQILANLANDYFDHKHGADTDRVGPLRVMQAGLVTDRQMRTAIGINIA
                     LAIAGGLYLVYRGGWPVLVLGLLAVICAVAYTGGPFPLGYHGLGDLFVFVFFGLVGVA
                     GSAYVQTRDLTWLAIIASLPIACLATAIIVANNLRDIETDRAAGKHTLATHLGRLGTI
                     REYQGLIAIAYLIPLLLAIFGDVPWAWLVIPVTLPLALPLVTAVQTGAGKGLIPVLVG
                     TSRLTFY"
BASE COUNT          476 a         1014 c          769 g          451 t
ORIGIN      
        1 gggagatcga tcggagctgg acatgagctg aaccctccac ggatgaacaa gtacaggaac
       61 ggccccggag acaccaagcg ggcaaaaacc caacagcgcc ggggccaaac tcaattctat
      121 agttctttat cgacaatgca acgaaatggc cccgactgcc gcgcatccgt gccaagcacg
      181 accgcagcca tgctaccgct cccctgtctg tcaccctctc aattccccta caaacccagc
      241 ccaaaccgag cccttttccg ttccctccac cctctaccct caaccaactt cgcgcctccg
      301 cgccttcccg tctccgcgcc tcacttgcct tgcggtcaac gccttcgtaa agtaaaatag
      361 aacatgcgtt cagggagccg gcactcctgt tcgcgacgcc gggtatcgcc gcaacgacgc
      421 tcgcttaccc ggcatctctc atttgccctg gcggcgctcc tggcccgccg gatcacccgc
      481 gtgaggtgaa tagccatgac cgtcagcaaa gcgtccgata tcaccgccaa gctgctcgaa
      541 ccatttccgg tcgacgtgat cgaggagaaa aacggtctcc tctatatccc ccacgagatc
      601 atccgcgacc ggctgatctc ggcgaccgac aaccagttcg actggtcgat cgaccagatc
      661 ctcttccggg acgacggtgt cacccgccgc gccaacgatc gctacaccgg cgagccgaag
      721 cgcccgctct caatgatcgt gatcggcacg ctgaccattc cgggtctcgg cagccgggcc
      781 ggcatcggcg cgcacccgct cgatgagggc gccggcgagg acgcggccta caagagcgcc
      841 gagtccgatg ccgttaagcg ggccgcgatg gccttcggcg tcggcctcca ccagctctac
      901 atcgaaaccg gcgaggccaa gcgggtccag aagccgcagc gcaacggcgg gcaggtccgc
      961 gccatggccc gtcctgcccc tcagcagcgg tcggagccgc gccagagcga gctcaagccg
     1021 gccaacgaca agcagttcgg caatgaggtc cgccaggcga tggccagccg cgatgcgacc
     1081 gcgttccgca agctggtcga cgacgccaac gaccacgtcg gccgctgggt cgcattggtg
     1141 caggccgccg agtcgcccca ggccctcgac tggatcaagc gccagatcga gcgcaagggc
     1201 gtcgaaagcg acatcctgac ccacgagatc cgcaagcgcg aagagcaaat cgccgcgtaa
     1261 gcggacgtca gtcgtcggac gtcagacgtc ggacatcagt aagaggaacg gggcggtcaa
     1321 attgaccgcc ccgttcttat gcgccatcgg tccgggcgaa cctgcacggg cgcattgcaa
     1381 tgcaccctcg accgctccga cgaccgcccg ggcgcttgcg ccccggctga aacctgaacc
     1441 cggctaaatc cggctgcgac gccattcgga atgtggcgcg ggtgcctctg tgccccgcga
     1501 tcgccggcag ggcgatgatg gtctcgccca ccgaccgcgt gcgttccgga cgactcggaa
     1561 acgtagcgcg gggttaccac accccgcttg gttgatctcc ctgcacgatt catagcgctg
     1621 acccggcaac agggttacca tgccccgcca cggacgggca gtgatcgggt ccgggcgggg
     1681 catggtaacc ccccgctacg aaatcggacg ggtgtttacg cccgggcggc gctggaatgc
     1741 ccatcccatg tatccccagt catcctggaa ccgaggtcgc attgccatgc acctctacaa
     1801 ccccgcgcct tcgcgccttc ctgccccaac tgctcgcctg ctccccgcca tacttccccc
     1861 atgacgatca cactctcgcc actcgaaatc tggctgcatg cgatccggcc gaagacgctc
     1921 ttcgcctcgg tctcgccgat catcgtcggc tcggcggtcg cgtatcacga ggatggcttc
     1981 cacctgccga cggccctgat cgccctggcg gttgcggtcc tcctccagat cctcgccaac
     2041 ctcgccaacg actacttcga ccacaagcac ggcgccgata ccgaccgcgt cggtccgctc
     2101 cgggtcatgc aggccggcct tgtcaccgac cgccagatgc ggaccgcgat cgggatcaac
     2161 atcgccctcg cgatcgccgg tgggctctac ctcgtctatc gcggcggctg gccggtcctc
     2221 gtcctcgggc tgctggcggt gatctgcgcc gtcgcctaca ccggcggacc cttcccgctc
     2281 ggctaccacg gcctgggcga tctcttcgtc ttcgtcttct tcgggctggt cggcgtggcc
     2341 ggctccgctt atgtccagac acgcgatctg acctggcttg cgatcatcgc ctctctgcca
     2401 atcgcctgcc tcgcgaccgc gatcatcgtc gccaacaacc tgcgcgacat cgagaccgac
     2461 cgcgccgccg gcaagcacac actcgccact cacctcggcc gcctgggcac gatccgcgag
     2521 taccaggggc tgatcgcgat cgcctacctg atcccgctcc tgctcgccat cttcggagac
     2581 gtgccgtggg cctggctggt gatcccggtc acgctgccgc tcgccctgcc gctcgtgacc
     2641 gcggtccaaa ccggcgccgg caagggcttg atcccggtcc tggtcggcac ctcccgactg
     2701 accttctact
//
LOCUS       DATAOZ010000423         5149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6747861, whole genome shotgun sequence.
ACCESSION   DATAOZ010000423 DATAOZ010000000
VERSION     DATAOZ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6747861"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VHR64_10870"
     CDS             <1..366
                     /locus_tag="VHR64_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3303373.1"
                     /translation="GPFSVDGIEITPLPVWHGQMRVFGFRIGDLAYVTDAKDVPAETR
                     ALMTGLDVLVLNALRERPHPTHLSLAEAVEIICELQPRRALLIHLSHELSHEAASRQL
                     PPGVEVAYDGLTVETAESD"
     gene            430..813
                     /locus_tag="VHR64_10875"
     CDS             430..813
                     /locus_tag="VHR64_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303374.1"
                     /translation="MAGFIGFFVGLVATIAFLVAGAPLASRRIAANSAYGLRSADTDG
                     DDQIWHLANAAIGRDLIMTAGVDALLTIVALVYWGDDSVQSALTVSILLISIAGVILA
                     IARGLTAARALGKAKQAFPPGMRRF"
     gene            913..2052
                     /locus_tag="VHR64_10880"
     CDS             913..2052
                     /locus_tag="VHR64_10880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3303375.1"
                     /translation="MKIAQVTPYDFAHPGGVAEHIQHLKTELNRLGHQVTIIAPRSRK
                     GGLEVGTDFYGVGRTVAIPGNKSRVRLTFDITLYSAVKEMLLREAFDVIHLHEPLTPV
                     LPYMVLLNSRAVNVATFHAYRFSNPWYSAFKPYMSFVLGRLDGRIAVSDAAREFVSQY
                     FEGPYSVIPNGIDTVRFGDGIQPFPWAMDGTPRILFVGRFNEARKGFKYLLRAMPLIQ
                     QQFPNARLMVVGPGDPRRVEGTIERHRIRNVDFFGQVSKEDLPRYYASSSLVCAPSIE
                     RESFGIILLEAMASGKPLVATNIPGYAGVMTSAHDGLMVLPQDSQALALAIVRLLADT
                     DLRVRLVDNGRITAESYAWPKVTSRVVEMYEQSVCSAAGARWRQE"
     gene            2060..2665
                     /locus_tag="VHR64_10885"
     CDS             2060..2665
                     /locus_tag="VHR64_10885"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX3303376.1"
                     /translation="MISERIGNLVRHRLLVIGHALAKIGFTPNLLTVIGVLLNLVVAI
                     IIATDHPQIGGFFLLVASAFDMLDGAVARASGTTSDFGGFLDSTLDRYSEAIVYGGVL
                     IYLLDTDDAELGSILVFVAAVGALLISYARARAEAAGYKASVGLVARPERVILLALGL
                     LIGKPIWALWILAFATHLTALTRIFHVWRASLAKSTPDPAR"
     gene            2684..2851
                     /locus_tag="VHR64_10890"
     CDS             2684..2851
                     /locus_tag="VHR64_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303377.1"
                     /translation="MIDREDSISPDEFEDVPVAEDDVRSAARSCSAILVIIAIVGAIA
                     CIMLGIALVYG"
     gene            complement(3142..4365)
                     /locus_tag="VHR64_10895"
     CDS             complement(3142..4365)
                     /locus_tag="VHR64_10895"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX3303378.1"
                     /translation="MRALEYQFSLPNYVAVRAADRLPLQLIERGTIPGLREIAPEQKP
                     LPGPEWTRIAPILTGICGSDISTILNRSSAALMPFTSFPLTPGHEIVGRVTEVGARTS
                     GISAGQRVVVMPLISCQMRGVEPCEPCARGEPGVCTKTTEGDFSAGMLIGFCRDLPGG
                     WSQEMIAHQSQIYAIPDEIPDRAAVLIEPFSVAIHAVLRDPPPDDAKVLIVGSGTIGL
                     LVLAALRLLGKQCDVTVLARHPKQVELAEKFGATRVLRRKSAGEAAQEVTGARRFKLI
                     KGGHTYAGGFDWVFDCVGSKTSVDEGLRVAGPHGHIVMVGCASEVPKLDLSYLWAREL
                     TVTGCYVYGREKSLPGEPHSFEVAIDLLAKCPDFGLDQMVTHTIPLSNWRTGFQTVLD
                     RKGSGAIKVVYDCQA"
     gene            complement(4371..>5149)
                     /locus_tag="VHR64_10900"
     CDS             complement(4371..>5149)
                     /locus_tag="VHR64_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX3303379.1"
                     /translation="PEAYFFTLEQGYYAIPYQGDPDSFISQVVDRLVPLATSQLIIEN
                     DFVPDLPEELWSGDEITESITRAGKHLDDLDLLPSPFPLEDYFSPEDFRHVKSLFQMG
                     GLSYGNLSARKDAARFWMSASGVDKSKLTEIGRDILLVTDYIPERRAMRLSVPANIQP
                     RRVSVDAIEHWMIYREHPSVGAILHVHAWMNGIKSTHINYPCGTFELAETVAELVRQE
                     PDPGHAVIGLRNHGLTITGESLDEIFERISGKILRQVPMN"
BASE COUNT          891 a         1652 c         1640 g          966 t
ORIGIN      
        1 ggccccttct cggtcgacgg catcgagatc acgccgcttc ccgtctggca cggacagatg
       61 cgtgtcttcg gtttccggat cggcgatctg gcctacgtca ccgacgccaa ggatgtcccg
      121 gccgagaccc gcgcgctgat gaccggtctc gacgtcctgg tcctgaatgc gctgcgagaa
      181 cgaccacatc cgactcacct cagcctggcc gaggccgtcg agatcatctg cgagctccaa
      241 cctcggcggg cgctgctgat ccacctcagt cacgagctga gtcacgaggc ggcatcccgg
      301 caacttccgc ccggtgtcga ggttgcgtat gacggcctga cggtcgaaac ggcggagtcc
      361 gactgagcgc aatccctgcg tcgtggtagc ctcaccagaa ctgaaacagc cgaaaggggc
      421 tggggagcta tggctgggtt catcggattt ttcgtcgggc tggtcgcgac gatcgcattt
      481 cttgttgccg gcgcgccgct ggcatcgcgg cggatcgccg cgaattcggc ttatggattg
      541 cgctcggccg ataccgacgg cgacgaccag atctggcacc ttgccaatgc ggcgatcggc
      601 cgggatctga tcatgaccgc cggcgttgat gcgttgctga ccatcgtcgc gcttgtctat
      661 tggggcgatg attcggtcca gagcgcgctg acggtctcga tcctcctgat ctcaatcgcc
      721 ggtgtcatcc tggccatcgc gcgggggttg accgcggccc gggcactggg caaggccaag
      781 caggccttcc cgccggggat gcgccggttc tgatcgattg gctgggagca tcctttatcc
      841 tatgcgcgtg aagcgttcta ccaaggcaat ctgtcgtcct gcccgttcgt tgctgtcccg
      901 gagttcacgc tcgtgaaaat cgcgcaggtc actccgtacg acttcgcgca ccccggcggt
      961 gtggccgagc atattcagca tctcaagact gagctcaatc gcctcggtca tcaggtcacg
     1021 atcatcgccc cacgatcccg aaagggcggg ctcgaggtcg ggactgattt ttatggcgtt
     1081 gggcggactg tcgcgatccc gggcaacaaa tcgcgcgtgc gcttgacctt cgacatcacg
     1141 ctctacagcg ccgtcaagga gatgctgctg cgggaggcct tcgatgtcat tcacctgcac
     1201 gagccgttga cgccggtcct gccatacatg gtcctgctca actcccgcgc ggtgaacgtg
     1261 gccaccttcc acgcctatcg cttctcgaat ccgtggtaca gcgccttcaa gccgtacatg
     1321 tccttcgtcc tcgggcgact ggacggtcgg atcgccgtct ccgatgccgc ccgggagttc
     1381 gtcagccagt acttcgaggg gccgtactcc gtcattccga acggcatcga taccgtccgc
     1441 ttcggcgacg gcatccagcc cttcccgtgg gcgatggatg gtacgccgcg catcctcttc
     1501 gtcggccggt tcaacgaggc gcgcaagggg ttcaagtacc tgcttcgggc gatgcccctg
     1561 attcagcagc agtttccgaa tgcccggttg atggtggtcg gcccgggtga tccgcgccgg
     1621 gtggagggga cgatcgagcg gcaccgcatc cgcaatgtcg attttttcgg acaggtctcc
     1681 aaggaggatc tgccgcgcta ctacgccagc agctcactgg tctgcgcgcc gtcgatcgaa
     1741 cgggagagct tcgggatcat cctgctcgag gccatggcct ccggcaaacc gctggtggcg
     1801 acgaacatcc cgggctacgc gggcgtgatg accagcgcgc acgacggtct gatggtgctg
     1861 ccgcaggact cgcaggccct cgccctggca atcgttcggt tgctggcgga caccgacctt
     1921 cgggtccggt tagtagataa tggccggata accgccgaaa gctatgcgtg gccgaaggtg
     1981 acaagcaggg tagtggagat gtacgagcag tcggtctgct cggcggccgg cgcacgatgg
     2041 cggcaggagt aaacaggaca tgatctctga gcgaatcggc aatctggttc gccatcgact
     2101 ccttgtcatc ggacatgccc tggcgaaaat cggcttcact ccgaacctgc tcacagtcat
     2161 cggcgtgctg ctcaacctcg ttgtcgcaat catcatcgcg accgatcacc cgcaaatcgg
     2221 cggcttcttc ttgctggtgg caagtgcgtt cgacatgctc gacggcgcgg tggcccgcgc
     2281 gtccggcacg accagcgact tcggcggctt cctcgactcg acgctcgacc ggtactccga
     2341 agctatcgtc tacggtggcg tgctgatcta cctgctcgac acggacgatg ccgagcttgg
     2401 ctcgattctc gtcttcgtgg cggctgttgg cgcgctgtta atcagctacg cccgggcccg
     2461 ggccgaggcg gccgggtaca aggcgtcggt cggcttggtg gcgcgccccg aacgggtgat
     2521 cctgctggca ttggggctcc tgatcggcaa gccgatctgg gcgctctgga tcctggcatt
     2581 cgcgacgcac ctgaccgccc tgacccggat cttccatgtc tggcgcgcat cactggccaa
     2641 gtcgactccc gatcccgccc ggtaggttgg ccagagacga accatgatcg atcgcgaaga
     2701 ctcgatttca cctgacgagt tcgaggatgt cccggtagcc gaagacgacg tccgttcggc
     2761 cgcgcggtca tgctcggcca tcctggtcat catcgcgatc gtcggcgcca ttgcctgcat
     2821 catgctcggg atcgcgctgg tctacgggta gtcccagtcg acactcctcc actcgccgct
     2881 tcgcacgatt cgtcgttcga ccgtccgatg cggcgaggag cgaacccata accgagaaca
     2941 ggaatcgacc tatacccgaa tcacagcagg tctccgatcg aacgaacctg tctgagaagg
     3001 ggcccgacga caagtccggc tcgaagtcga tgcacgccgt tccgccgaat cgcaacccgg
     3061 ctcagggcga gcgcgaggca gaacccgacg acgagaatat ccgcagcacc gagtcgaagg
     3121 gcgctaacgc gtgaggcgtc gtcaggcctg gcagtcgtag acgaccttga tcgcgccgct
     3181 gcccttgcgg tcgagcacgg tctggaagcc ggtccgccaa ttcgaaagcg gaatcgtgtg
     3241 cgtgaccatc tggtccagcc cgaagtcggg gcatttcgcg agcagatcga tcgcgacctc
     3301 aaaactgtgc ggctcgcccg gcagtgactt ctcgcgcccg tagacgtagc atccggtcac
     3361 ggttaactcc cgcgcccaga gataggaaag gtcgagcttc ggcacttcgg acgcgcagcc
     3421 gaccatgaca atgtgcccgt gtggcccagc gacccgcagg ccctcgtcga ccgacgtctt
     3481 cgagccgacg caatcgaaga cccagtcgaa cccgccggcg taggtgtggc cgcccttgat
     3541 caacttgaag cgacgagcgc cggttacctc ttgtgcggcc tcaccggccg atttccggcg
     3601 gaggacacgg gtcgcgccga acttctcggc caactcgacc tgctttggat ggcgggccag
     3661 cactgtcacg tcgcactgct taccgagaag ccgtaatgcc gcgaggacga gcaggccgat
     3721 cgtcccgctg ccgacaatca gcaccttggc gtcgtcgggc gggggatcgc gcagcaccgc
     3781 gtggatcgcc acgctgaacg gctcaatcag gacggcggct cgatccggaa tctcgtccgg
     3841 aatcgcgtag atctgcgact gatgcgcaat catctcttgc gaccagccgc cgggcagatc
     3901 gcggcagaaa ccgatcagca tgccggccga gaaatcgccc tcggtcgtct tcgtgcagac
     3961 acccggctcg ccacgcgcgc acggctcgca cggttcgaca ccccgcatct ggcaactgat
     4021 caacggcatg acgactactc gctggccggc gctgatcccg ctggttcgtg caccgacctc
     4081 ggtcacgcgc ccgacgatct cgtgacccgg cgtgagcggg aaggaggtga agggcatcag
     4141 ggccgcgctg gaccggttca ggatcgtgct gatgtcgctg ccgcagattc ctgtcaggat
     4201 cggggcaatc cgggtccatt ccggtccggg cagaggcttc tgctctgggg cgatctcccg
     4261 gaggcccggg atcgtgccgc gctcgatgag ctggagcgga aggcgatcgg ccgcccgcac
     4321 cgcgacatag ttcggaagcg agaactggta ctcgagcgcg cgcatcggcg tcagttcatg
     4381 ggaacctggc gcaggatctt gccgctgatg cgttcaaaga tctcgtccag gctctctccg
     4441 gtgatcgtca ggccgtggtt gcgaaggccg atcacggcgt ggcctggatc cggctcctgc
     4501 cggaccagct cagccactgt ctcggccagc tcgaaggtgc cacacgggta attgatgtgc
     4561 gtcgacttga tcccgttcat ccaggcgtgg acgtggagga tcgcgccgac cgacggatgc
     4621 tcgcggtaga tcatccagtg ctcgatcgcg tcgaccgaga cgcggcgcgg ctggatgttg
     4681 gccggcacgc tcagtcgcat cgcgcggcgc tcgggaatgt agtcggtcac gagcaggatg
     4741 tcgcggccga tctcggttaa cttgctcttg tcgactccgc tggcactcat ccagaagcgg
     4801 gcagcgtcct tgcgggcgct caggttgcca taggagagac cgcccatctg gaaaaggctc
     4861 ttgacgtggc ggaaatcctc cggcgagaag tagtcctcga gcgggaaggg cgacgggagc
     4921 agatcgagat cgtcgaggtg cttgccggcg cgggtgatcg attcggtgat ctcatcgccg
     4981 ctccagagct cctccgggag atccggcaca aaatcgtttt cgatgatcag ctgcgacgtc
     5041 gccagcggca ccagtcgatc gacgacttgc gagatgaacg aatcagggtc accctggtac
     5101 gggatcgcgt agtaaccctg ctcgagcgtg aagaagtagg cctccggcc
//
LOCUS       DATAOZ010000424         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2802322, whole genome shotgun sequence.
ACCESSION   DATAOZ010000424 DATAOZ010000000
VERSION     DATAOZ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2802322"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VHR64_10905"
     CDS             <1..792
                     /locus_tag="VHR64_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3303380.1"
                     /translation="GSTRFILPAATNDEIQKLPWLIPHFATEEGRLKMSIHSLVVETP
                     TRRIVVDTGLGNDKQGRGVPVWNNRSTPFLETMAAAGFPPNSIDTVLCTHLHVDHVGW
                     NTKLVDGKWVPTFPKARYLFGETEYEYWRDYTAEPDKVAVFNDSVKPVVDAGRVELIP
                     GDHRLCEEISVIPTPGHSPGHMSVLIESGGEQGLLTGDVAHHPCQMSHLDWCSVIDTD
                     PAQSAATRHKVFSRFADTPTLVIGGHYSAGHIRRDGDAFRFVALG"
     gene            1030..1872
                     /locus_tag="VHR64_10910"
     CDS             1030..1872
                     /locus_tag="VHR64_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3303381.1"
                     /translation="MKLVRYGEKGAEKPGLIDKSGQLRDLSAHLKDLTGEAYSPESLK
                     KLAGLDPASLPAVSGKPRFGAPVTSISKFVAIGLNYSDHAKETGAAIPTEPIIFMKAN
                     TSLSGPNDAVEKPRGSTKLDWEVEIAAIIGTRAKYVSEADALNYVAGYCVCNDVSERH
                     FQTERLGQWTKGKSHDTFGPVGPWLATKDEIKDVQNLSMWLDVNGQRRQTGSTQTMIF
                     SMAKCVSYVSQFMTLLPGDIITTGTPPGVGLGMKPPTFLNVGDVVTLGIEGLGEQRQE
                     IVAA"
BASE COUNT          400 a          705 c          615 g          343 t
ORIGIN      
        1 ggctcgaccc gcttcatcct gccggccgcg accaacgatg aaatccagaa gctgccctgg
       61 ctgatcccgc atttcgccac cgaagagggc cgactgaaaa tgtcgatcca ctcgctggtg
      121 gtcgagacgc cgacgcgccg catcgtggtc gacaccggtc tcggcaacga caagcagggc
      181 cgcggcgtcc ccgtctggaa caaccgcagc acgccgtttc tggagaccat ggcggcggcg
      241 ggctttcccc ctaacagcat cgacaccgtg ctgtgcacac atcttcatgt tgaccatgtc
      301 ggctggaata cgaaactggt cgacggcaaa tgggtgccga ccttcccgaa ggcgcgctac
      361 ctgttcggcg agaccgaata cgagtattgg cgcgattata cggccgagcc ggacaaggtc
      421 gcggtgttca acgattccgt gaagccggtc gtcgatgccg gccgggtcga gctgatccca
      481 ggcgatcacc ggctctgcga ggagatcagc gtgatcccga cccccggcca cagccccggt
      541 cacatgagcg tcctgatcga gtccggcggt gagcaggggc tgctcaccgg cgacgttgcc
      601 catcatccct gccagatgtc gcatctcgac tggtgctcgg tcatcgatac cgatcccgcc
      661 caatcggcgg caacgcggca caaggtgttc tcgcgctttg ccgacacccc gacgctggtg
      721 atcggcgggc attactcggc cggacacatc aggcgggatg gggacgcgtt caggttcgtg
      781 gcgctgggat gagacgcaat cgtagggtgg gcaaagccac cgggtcgcgc gaatgcgcgc
      841 ccgatgacag gctccgcgtg cccaccatac cgggccatca aaaagaattc gtgggcacgg
      901 cgctccgcgc ctttgcccac cctacaaagg aagggctgca atgcgccccg ttttgagcgg
      961 ttgaatttcc ggccgccctg ttccatgaag ctaattaacc gatcggtcca tctccaggga
     1021 gaaacgagaa tgaagcttgt tcgttatggc gaaaagggtg cggaaaagcc cggcctgatc
     1081 gacaaatccg gccaattgcg cgacctgtcg gcgcatctga aggacctgac cggcgaggcc
     1141 tattccccgg aatccctgaa gaagctggcg ggccttgacc cggcctccct gcccgccgtc
     1201 tccggcaagc cgcgcttcgg cgcgcccgtc accagcatct cgaaattcgt cgccatcggc
     1261 ctcaactaca gcgaccacgc caaggaaacc ggcgccgcga tcccgaccga gccgatcatc
     1321 ttcatgaagg ccaacacctc gctgtccggc ccgaacgacg ctgtcgagaa gccgcgcggc
     1381 tcgaccaagc tcgactggga agtcgagatc gccgccatca tcggcacccg cgccaagtat
     1441 gtctcggaag ccgatgcgct gaactacgtc gccggctatt gcgtctgcaa cgacgtctcc
     1501 gagcgccact tccagaccga gcgcctgggc cagtggacca agggcaagtc gcacgacacg
     1561 ttcggcccgg tcggcccgtg gctcgccacc aaggacgaga tcaaggacgt gcagaacctg
     1621 tcgatgtggc tcgacgtcaa cggccagcgc cgccagaccg gctcgaccca gaccatgatc
     1681 ttctccatgg ccaaatgcgt ctcctacgtg tcgcagttca tgaccctgct gccgggcgac
     1741 atcatcacca ccggcacccc gcccggcgtc ggtctcggca tgaagccgcc gaccttcctc
     1801 aatgtcggcg acgtcgtcac gctcggcatc gaaggcctcg gcgagcagcg ccaggagatc
     1861 gtcgcggcgt aagcttccgc cctcatcctg atgagcgcgc aacgcgcgcg tctcgaagat
     1921 gcgccaagag tacagtcacc cgacgttcgc gccgggtgac ccaagaacac ctcccgttgt
     1981 ccatccttcg aggcgcgcgc caagctgcgc gctcctcagg atgcggaccg tctgtttgga
     2041 tagaggttac tccccatgaa act
//
LOCUS       DATAOZ010000425         6327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3068075, whole genome shotgun sequence.
ACCESSION   DATAOZ010000425 DATAOZ010000000
VERSION     DATAOZ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3068075"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VHR64_10915"
     CDS             complement(<1..322)
                     /locus_tag="VHR64_10915"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX3303382.1"
                     /translation="MTKNETIRYVALLRGINVGGHRMIKKDELQAIFEGCGFTDVKTV
                     LASGNVVFSSDLVDEAAIQERIETALHDALGYTVNVMVRTTAYLQDLIALDPFGEVGP
                     GDGHN"
     gene            601..1140
                     /locus_tag="VHR64_10920"
     CDS             601..1140
                     /locus_tag="VHR64_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX3303383.1"
                     /translation="MSQVVDPAHVDGNEIDLDVVRDILSRFTYHDFQEAQELILGALM
                     EVNSHFGWVSLEAAKVVADYFGTSENRVYGLLTFYADFRTQPRGKHFMLLCHGMSCYV
                     MGSQKLINELEQKYGIVDGGTTADGELTVQVVNGCLGVCDKAPIVELDHEFHTELTVE
                     KFNDVLQRAIANGGGWRHH"
     gene            1115..2893
                     /locus_tag="VHR64_10925"
     CDS             1115..2893
                     /locus_tag="VHR64_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX3303384.1"
                     /translation="MAADGDTTDMHVQGDTTLPRLTSRLDFQQYEATAKDKWNSLWSS
                     TNTVVTVGVGSSSIAKGSEKVLDAAAEYALMKSNVVVRKTGVDGADWMEVQVTVKRPG
                     APLVYYANVTPDEIPDLIEGRLESKALAVDGDQPYNNLPPLKDHPFFKYQQRLILRDV
                     GVIDPDSIEEAIAKGAYSAYFKVLFEMSQEEVIAEMTAANLRGRGGAGFPAGIKWESG
                     RKARNSPKFIVCNSHEGEPNVYKDRRILEGDPHRILEGLLIAAITVGAERAYNYIGGE
                     HPWAIKRFRRAVADAERLGLIGKNVLGSGKNVAFRVRTGGGAYICGEGSALMYSVMGV
                     RGQPRTKPPRSVEEGLWKRPTVANNTETMANVRDIVNNGGAWYASIGTEKSSGTKLMT
                     VQGPLKTMGLVEVPMGITLRTLIEEVWGGMRDGYKFKGIQTGGVSAGPLKEEHLDLPV
                     DFDSLTHVGGMLGSGGFVVFDESVSAVDFARYLTAFNRYESCSKCVPCARGNPALVEI
                     IDRIRLGRGTLADLPLIRRTSKHVIELSLCGLGQVAPMPLLGMMDQYPEEFETAVTNN
                     TGTVLWLTQRQERVLSTNTTTTPAAD"
     gene            complement(3188..3748)
                     /locus_tag="VHR64_10930"
     CDS             complement(3188..3748)
                     /locus_tag="VHR64_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3303385.1"
                     /translation="MRRVIVSLVVVTLVAFGLLGATRSPHYVFGQSASPSPGEQVPEG
                     VSFQFIAYGQLPPDMQAPEGFSMFRLALDPGASFPADKNDPTTALGYVEKGTLTLMLD
                     MDITVLHAGTENQEPTAQDFEMMPANQRFTMTVGDSAIFPANTAGTVTNDGAEQAILL
                     IAQLEPPGYSEGEMATPGAGMGTPSS"
     gene            4304..4606
                     /locus_tag="VHR64_10935"
     CDS             4304..4606
                     /locus_tag="VHR64_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303386.1"
                     /translation="MYTVIRQYTGNSLRDIIKRDEASLRQTMTAVPGFRGYYMVETKD
                     GLATITVCDDMKGTQESNKRAAEWVKDHVPASANLSKPTIFEGETLMSIDARQPVR"
     gene            4931..5362
                     /locus_tag="VHR64_10940"
     CDS             4931..5362
                     /locus_tag="VHR64_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303387.1"
                     /translation="MSGLRIPWRQPRTFASYDLPERELVTALPGVADRASARVWRITL
                     KPGQAEHLRFEGRSSILVESGKMTIQYVPGSDTEIVATGPNLPFVDKHGIPVESWVME
                     TYQPPYLTRFQGLVRDSGDFGMVANDANEDLVAIVVERARI"
BASE COUNT         1216 a         2054 c         1869 g         1188 t
ORIGIN      
        1 agttgtgccc atcccccgga ccaacctcgc cgaacgggtc gagcgcgatc agatcctgca
       61 aataggcggt cgtccgcacc atcacattaa ctgtgtaccc aagcgcgtcg tgcagtgccg
      121 tctcgatccg ctcctgaatc gccgcctcat ccaccagatc gctgctgaag acgacatttc
      181 cgctcgcgag cacggtcttg acatcggtaa atccgcaccc ctcgaagatc gcctgcagct
      241 catccttctt gatcatgcga tgcccgccaa cattgatccc ccgcaatagg gcaacgtatc
      301 tgatggtctc gttcttggtc attggtctct cgtctttggt cgttggtcgt tggtcgttgg
      361 tcgttagtcg ttagtcgtta gtcgttagtc gttagtcgtt agtcgttgga tcgagcgtac
      421 tggcttcctt cctgttcaat ctatcaacgt ccaacgtcca tccacctcta tcaacgtcca
      481 gtaaacgcct gcatgcccta actgctcgcc tgctcgcctc ctcgcctgct cgcctccttt
      541 atactcatct ctgcggtccg cgtaccggcc gctcgcatgc cgttcacaag gagttaccgc
      601 gtgtctcagg tggtagaccc cgcccacgtc gacgggaacg agatagatct cgacgtcgtg
      661 cgcgacatcc tttcccgctt cacgtaccac gatttccagg aggcgcagga gctcatcctc
      721 ggagccctca tggaggtcaa ctcgcacttc ggatgggtct ccctcgaggc cgccaaggtc
      781 gtggcggact acttcggcac cagcgagaac cgggtctacg gcctgctgac cttctacgcc
      841 gactttcgga cccagccccg cggcaagcac ttcatgctcc tgtgccacgg aatgtcctgt
      901 tacgtgatgg gctcccagaa gctcatcaac gagctggagc agaagtacgg catcgtcgat
      961 ggcggcacga cggccgacgg cgagttgacc gtccaggtcg tcaacggctg cctcggtgtc
     1021 tgcgacaagg cgccgatcgt cgagctcgac cacgaattcc acaccgagct gaccgtcgag
     1081 aagttcaacg acgtcctgca acgagccatc gcaaatggcg gcggatggag acaccactga
     1141 tatgcacgtc caaggtgaca cgacccttcc ccggctgacc tcccgcctcg atttccagca
     1201 atacgaggcc accgccaagg acaagtggaa ttcgctctgg tcctccacca acacggtggt
     1261 caccgtcggg gttggcagct cgtcgatcgc aaagggctcc gagaaggtgc tcgatgccgc
     1321 cgccgagtac gcgctcatga aatcgaacgt cgtggtccgc aagaccggcg tcgatggcgc
     1381 ggactggatg gaggtccagg tcacggtcaa gcggcctggc gccccgctgg tctactacgc
     1441 caacgtcacg ccggatgaga tccccgatct catcgagggc cggctcgaat cgaaggcgct
     1501 tgccgtcgat ggcgaccagc cctacaacaa cctgccgccg ctcaaggacc atccgttctt
     1561 caagtatcag cagcgactca tcctcaggga tgtcggcgtg atcgatcccg attcgatcga
     1621 ggaagcgatt gccaagggcg cctactccgc ctatttcaag gtccttttcg aaatgtccca
     1681 ggaagaggtc attgccgaga tgaccgccgc caacctgcgc ggtcgcggcg gcgcgggctt
     1741 cccggccggc atcaagtggg agagtggccg caaggcgcgc aacagcccga agttcatcgt
     1801 ctgcaacagc cacgagggcg agccgaacgt ttacaaggac cgccgcatcc tggaaggcga
     1861 cccgcaccgg atcctcgagg gcttgctgat cgcggccatc acagtcggcg cggaacgggc
     1921 ctacaactac atcggcggcg agcacccgtg ggccatcaag cggttccgcc gggccgtcgc
     1981 ggacgccgag cgactcgggc tgatcggcaa gaatgtgctc ggctcgggca agaacgtcgc
     2041 cttccgggtc cgcaccggtg gcggcgccta catctgcggc gagggctcgg ccctcatgta
     2101 ctcggtcatg ggcgtgcgcg gtcaaccccg caccaagccg ccgcgatcgg tcgaggaggg
     2161 cctctggaaa cgcccgaccg tcgccaacaa caccgagacc atggccaacg tccgcgacat
     2221 cgtcaacaac ggcggcgcct ggtatgccag catcggcacc gagaagagct ccggcacgaa
     2281 gctgatgacc gtccagggtc cgctcaagac gatgggcctc gtcgaggtcc cgatgggcat
     2341 caccctgcgt accctgatcg aagaggtctg gggcggcatg cgggatggct acaaattcaa
     2401 gggcatccag accgggggcg tctcggccgg accactcaag gaggagcacc tcgacctgcc
     2461 ggtcgatttc gactcgctga cccacgttgg aggcatgctc gggtcgggcg gattcgtcgt
     2521 cttcgacgag tcggtcagcg ccgtcgattt cgcgcgctac ctgaccgctt tcaaccggta
     2581 cgagtcctgc tcgaagtgcg tgccctgcgc gcgcggcaac ccggcgctgg tcgagatcat
     2641 cgaccggatt cgcctcggcc gcggcaccct ggccgatctg ccgctgatcc ggcgcacgag
     2701 caagcacgtc atcgagctct cgctctgcgg tctcggccag gtcgcgccga tgccgctgtt
     2761 gggcatgatg gaccagtatc ccgaggagtt cgagaccgcg gtcacgaaca acaccggcac
     2821 cgtgctctgg ctgacccagc gccaggagcg agtgctgtcc accaacacca caacgacgcc
     2881 ggccgctgac tagcgccaag gcgagggaga tcaggcaaca gcgcgggaca attcccgcgc
     2941 tgttttgctg cgatttcctg gatgcatcgg cctgctcatc acgtagggcg gtattaccat
     3001 ggcccgctga tcacgccagg aaattgcctc gataggaatc caatgccaac tgcatctcgg
     3061 ctccatccac cgggcgtggg gatgcgtcaa gcggagcgtg gtaaccccgc gctacgttcc
     3121 cgtttggcac tcggcttcgg cggagcacat tggatcggtc atattcatag ggatatgtcg
     3181 tatgggatta gctgctgggc gtccccatgc cggccccggg tgttgccatc tcgccctccg
     3241 aataaccggg cggctcgagc tgggcgatca gcagaatggc ctgctcggcg ccgtcgttgg
     3301 tcaccgtacc agcggtattc gccgggaaga tcgccgagtc tccaacggtc atcgtgaacc
     3361 gctggttcgc cggcatcatt tcgaaatcct gcgcggtcgg ctcctggttc tcggttccgg
     3421 catgcaagac ggtgatgtcc atgtcgagca tcagcgtcaa cgtgcccttc tccacgtaac
     3481 caagcgccgt ggtcgggtcg ttcttgtcgg cggggaatga tgcgcccgga tcaagtgcga
     3541 gccggaacat cgagaaccct tccggcgctt gcatatccgg tggtagctgc ccatatgcaa
     3601 tgaactggaa tgagaccccc tccgggacct gctcccccgg cgatggactt gcgctctgtc
     3661 cgaatacgta atgtggggat cgggtcgccc ctaacaggcc gaacgccacc agcgtgacga
     3721 caacgagcga cactatgact cgacgcatgg ctagcctcct gtccgcgcga gatggaaatc
     3781 acactacccg cgaccgatcg atcaggcacc ctgccagtga ccggcacgac ggaacaacac
     3841 aagtccgcgg cgaagcgtga cgccgcggac ttgtgccggg gtgggaaggg aatacgccgc
     3901 agtgcttgcc ttcggactcg gtcacgctat acccgcgcct tgaccggcac aactgtccgt
     3961 acgtggccgg ctccaaaatt gagtaggaat actcatgacc gaatccggaa cctgcctgga
     4021 tgcgatgtcc gggcaatgcc aaaaaaagta ggaccgggcg gctcgcccgg ttttgtcgtc
     4081 ggtggatgcc gggcaagccg cccggcacta ggtcaattct cgaccgaacg cgaagctaca
     4141 ttacggcccg gagaattgcg caaaattgcc tgccccgact cgccaaatcc agtttgttga
     4201 tcttgacttc atggcacgtg aggcgcagca tggcggcctg cctctccaag tcgtctgcgg
     4261 ccgtcgctcc ggtttcgggg aggttctgtc tggaggatgc ggaatgtaca cggtcattcg
     4321 acagtacacc ggcaacagcc tgcgagacat catcaagcgc gatgaggcga gccttcgcca
     4381 aacgatgacg gctgtaccgg gcttccgggg ctactacatg gtcgagacga aggatggcct
     4441 tgccaccatc actgtctgcg acgacatgaa aggcacgcag gagtcgaaca aacgggccgc
     4501 cgagtgggtc aaggaccacg taccggcttc ggccaacctg agtaagccga cgattttcga
     4561 gggcgagacg ctcatgtcga tcgacgcccg ccagccggtt cggtagcgcg gacccgcgtc
     4621 tggcacctcg ctaatcttcc tcagcgagcc atcggacacg ccggaatccg gcagtttggt
     4681 ggctcttcgt gtgcccgcaa tcggtaactc agccgtcatc cgctattcca ccggtgacga
     4741 cttcatctgc gcttcacctg ttcgtgtgca aatagtcgca ttcggattaa tagtgacaga
     4801 caccgttttc ttcgctaaag tcaacgtggc ggcactgacc tgagccgtat gccatcgcac
     4861 gggggagcgc tctggtatgc aagaacggtc gatctcaatc acgcgtcgtt tctcaggttc
     4921 cggatcacca ttgagcggct tgcggattcc gtggcggcag ccccggacat ttgcgagcta
     4981 tgacctgccc gaacgcgagc tggtcacggc gctgcccggc gtggcagacc gggccagcgc
     5041 gcgcgtctgg cggatcacgc tcaagcccgg ccaggccgag cacctgcggt tcgagggacg
     5101 atcctcgatc ctggtcgagt cgggcaagat gacgatccag tatgtgccgg gcagcgacac
     5161 cgagatcgtc gccaccggcc ccaacctgcc atttgtcgac aagcacggga tcccggtcga
     5221 gtcatgggtc atggagacct atcaaccgcc gtacctgacc cggttccagg gtctggtccg
     5281 ggacagcggg gacttcggca tggtcgcgaa cgatgccaat gaggatctgg tcgcgatcgt
     5341 ggtcgaacgc gccaggatct aagcggatcg cacaccccaa tcacgaagtt gtggcgcgcc
     5401 ggttcgcgtt gtcacgaaat tgacatgcgc gggcgtctac tctgctggca tcgggtccgg
     5461 ggccagcgcc gacaccgcag ctgccaatcc aacaggcgct ggttccgccc acccgactag
     5521 ccttgtgctc tcgcgcggca actgcctctg tcgcgcaagc aatcgctccc cagcgatacg
     5581 gggccgggcc aaccagtccg gccccactct cttccccgga acccgccgct cgacacggcc
     5641 tggccagccc ggctccgatc acttgagttg ggatatgtcg tcaatggggc aatcgcctcc
     5701 attcattctc agatggggga atcagttccg gtattttcgc atctcaattg attcgtggat
     5761 cacgaacgag acagttaatg accgccctcg ctgaaattgc tcgtttgatg gtgcgttagt
     5821 gcccggcctg gcggataccg ccaatgggaa cgctcccgca ataaccggtc aaccagattg
     5881 cggaaagcag ctcctgaggg cctcccgaaa aactcccatg atttcggtga tttgcgcgac
     5941 caagtcgggt gtaaatctgg tgcacaggct ccgtaaccta ttgcaatctg gatattgtat
     6001 gtggttcaat atgcctacct ctccgactct tttacggcac caacggtcat cgttggcgcc
     6061 cggacacaga cccaaaagcg gagcaacgca acgggtgacg cgaccagcca cgccgatccc
     6121 gccaatcccg caacccgcgt cggagctgat cccagccagt cagttccaag gacgcaaaac
     6181 gtgcgaaacg gggatggccc cagccagcca tccccgtttc gctgcgacaa actccacgca
     6241 ctcgacatcc cgaacctcca ccagcacgga cgttgcctgg cttgaaaacg tagtgccggg
     6301 cggctcgccc ggttttgtcg tcggtgc
//
LOCUS       DATAOZ010000426         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5698489, whole genome shotgun sequence.
ACCESSION   DATAOZ010000426 DATAOZ010000000
VERSION     DATAOZ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5698489"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..2215
                     /locus_tag="VHR64_10945"
     CDS             431..2215
                     /locus_tag="VHR64_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX3303388.1"
                     /translation="MALRIEDYAIVGDTETAAVVGNNGSIDWLCLPRFDSAACFAALL
                     GDEQNGHWQIAPAGDATQVERQYRPGTLILETTFETTDGAVRVIDCMPPREGTPDVIR
                     IVEGLRGRVPMRLDLRPRFDYGRRIPLLRRVPGGVIATAGPDALFFKTSIQVDVSDGC
                     LSSEFPISEGQQLRFRLVWFPSHLEDPKQADPLSELNRTESWWQDWSSRCTYDGPYRE
                     TVLRSLITLKALTYAPTGAIVAAATTSLPEEVGGVRNWDYRYCWLRDSAFTITALSRF
                     GYTDEAIALGAWLRRAVAGDPDQMQIMYGIGAEQRLTEFELPWLSGYEGSGPVRVGNA
                     ASDQFQLDIYGEVMAAAYRAALAGVRPRENATKSPGLNLEAIVDAVEQRWRDPDEGIW
                     EVRGQRQHFTYSKFAAWYAVDRALKLAEIIGRDVPIERWLQMRNEIHDDICQHAFNAD
                     KGSFVQYYGANTLDASLLLIPGSGFLPDDDPRIVGTVNAVERELASGPFVSRYSTGEG
                     VDGLAGTEGAFLICSFWLVDALAHVGRTEDARRNLEALLALQNDVGLLSEEYDPTAGR
                     LLGNFPQAFSHIGLLSSILAVENGHIRP"
     gene            complement(2236..3573)
                     /locus_tag="VHR64_10950"
     CDS             complement(2236..3573)
                     /locus_tag="VHR64_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX3303389.1"
                     /translation="MSEREWVVYLAVVVFFSVFAAWFGRYSVTMPMFLVLVGALVGPD
                     ALGWLDIPSSAATSELVLELTLAMILFADASTLDLKKVRLDAGLPTRLLAIGLPLTVL
                     LGAAVAYVLFRDEGIGFALLMGAILAPTDAALGLPIFTNPRVPGRIRQALNVESGLND
                     GLATPLVTLFTALAVHDLHSGPSSWFVDSLSDISLAVLVGAAGGLLGGWLFRTAVRNT
                     WTTPVAQRIGNLGLALGIYWASLSVGGNGFVAAFVGGLVFGAATRYELHEATDFTEEC
                     GAVLSTLVWTIFGTTLVVQLLKSFNGRAFVFAILALTLVRMVPVAISLIGTGLRRDTV
                     LVMGWLGPRGLASVVFMLISFEALRESEQEIDTLAAMAGWAILLSVVLHALSAAPLAH
                     WYANRLQAAPPDAAEFLPSTELEVHHQRTMHRSFGNHQAHSAALQSAETVEIR"
BASE COUNT          758 a         1135 c         1118 g          669 t
ORIGIN      
        1 gatgccaaat ggccgagtcg actgtggcac tggaatgaac gtcggcacgg attggggccg
       61 cttcaacgac cggtggatcg gtccacattt ccgggcgcca ttgccgattc tggggtcgag
      121 gcagcaccca ggtcgttcga cgcgcattga gtgcctgact ggcgcactca ggcggaccct
      181 gcgctcccgc agatcgactg agaggtatct gcgccaccag ccgtactatc ggcctgaccg
      241 gtcggatacg catcatgcga agggtgtaat tgcggcccaa gtatggtccc ggccgattgt
      301 caagcccaca ttgggcccgg acgatgagat tggtgaaacg cattcacaac gctccggccc
      361 aacctcagcg gtcactttcc ccggaccgca gcgtgtcgac tcgaaccgag ataacccaag
      421 gagggagacg atggcactcc gcatcgagga ctatgcaatc gtcggagaca ccgaaacggc
      481 tgctgtcgtt ggcaacaacg gctccatcga ttggctctgc cttcccagat tcgattcggc
      541 cgcctgcttt gccgcactcc tcggtgatga gcaaaacggc cactggcaga tcgcgccggc
      601 cggtgatgcg actcaggttg agcgccaata ccggccgggc actttgatcc tggaaaccac
      661 gttcgagacc accgacggcg ctgtccgcgt catcgactgt atgccgccga gggagggtac
      721 tcccgatgtg atccggattg ttgagggtct gcgcggtcgt gtgccgatgc gactggacct
      781 gcggccgcgg ttcgattacg gccggagaat cccgctcctg cgccgggtgc cgggcggggt
      841 catcgcgacc gccggaccag atgcgctctt cttcaagaca tcgatccagg tggatgtcag
      901 cgacggttgt ctgagctcgg aatttccgat ctcggaaggt caacagcttc ggttccgcct
      961 ggtctggttc ccatctcacc tggaagatcc gaagcaagcc gatccacttt ccgagctcaa
     1021 ccgaactgag tcgtggtggc aggattggtc cagccgctgc acctacgacg gtccgtatcg
     1081 ggaaactgtt ctccgctcat tgatcacgct gaaagctctc acatatgcgc cgaccggagc
     1141 catcgtggcg gccgcgacga cgtcgctccc tgaagaggtc ggtggagtcc ggaattggga
     1201 ttaccggtat tgctggctcc gcgacagcgc gttcacgatc acggcgctgt cgaggtttgg
     1261 ctataccgat gaagctatcg cgttgggcgc ctggttgcgc cgcgccgtcg ccggagaccc
     1321 tgaccagatg caaatcatgt acgggatcgg cgccgagcag cggttgacgg agttcgagct
     1381 tccctggctt tcgggctacg aaggctcagg acctgttcga gtcggaaatg ccgcaagtga
     1441 ccagtttcaa cttgatatct acggagaggt catggcggct gcgtaccggg cggcattggc
     1501 aggcgtcagg ccccgggaga acgccaccaa atcgcccggg ctcaacctgg aagccatcgt
     1561 tgatgccgtc gagcagcgct ggcgtgatcc cgatgaaggt atctgggaag tccgtggtca
     1621 gcgtcagcac tttacctatt cgaagttcgc ggcctggtat gccgtcgacc gcgcattgaa
     1681 actcgccgag atcatcgggc gggatgtgcc aatcgagcgt tggctgcaga tgcggaacga
     1741 gatccacgac gacatttgcc agcacgcctt caatgcagac aaagggtcgt tcgttcagta
     1801 ttacggtgcc aataccctcg acgcgagcct gctcctgatt cccggcagcg gattcctccc
     1861 tgacgacgat cctcgcatcg tcggcacggt caacgccgtc gagcgcgaac ttgcttcagg
     1921 gccattcgtc tcgcgttact ccaccgggga aggcgtcgac ggtctcgccg gaacggaagg
     1981 ggcgttcctg atctgctcat tctggctcgt cgatgcgctg gcccatgtag gtcgcacgga
     2041 ggacgcgcgg cgaaacctgg aggcgttact cgcccttcag aacgacgtag gtctgctgag
     2101 cgaggaatac gatccgactg ccgggcgatt gcttggcaat ttcccgcagg cgttttcgca
     2161 cataggcctc ttgtcttcga tcctggccgt cgaaaatggc cacattcgtc cttagacgct
     2221 tctgcagaag aggcgctacc gaatctcgac cgtttccgcg ctttgaagag cggccgagtg
     2281 cgcctgatgg tttccgaagg accgatgcat cgtccgttgg tggtgcacct ccagctcagt
     2341 gctgggcaga aactcggccg cgtccggcgg ggctgcctgg agtcgattcg cataccaatg
     2401 agcaagggga gcggccgaga gcgcgtgaag cacgacactc aagagaatcg cccatccggc
     2461 catcgcggcc aaggtgtcga tttcctgctc agactcccgg agcgcttcga acgagatcaa
     2521 catgaacacc accgacgcga gacctcgggg acccagccag cccatcacga gtacggtatc
     2581 cctccggagt ccggtcccga tcaggctgat tgcgactgga accatgcgca cgagggtgag
     2641 cgcgaggatg gcaaacacga acgcccgccc gttgaacgat ttcagcaatt gcacgaccag
     2701 cgtcgtcccg aagatcgtcc agacgagcgt cgatagaact gccccgcact cctccgtgaa
     2761 atccgtcgcc tcatgaagct cgtagcgcgt tgccgcgcca aagacaagtc cgccaacgaa
     2821 cgcagccacg aagccattgc cgcccacgct cagcgatgcc caataaattc ccagggctag
     2881 tcccaggttg ccgatccgtt gcgctacggg tgtggtccac gtgttccgaa cggcggtccg
     2941 gaacagccaa ccacccagca ggccgcctgc agcgccaacc agaacggcaa ggctgatgtc
     3001 ggagagcgaa tcaacgaacc acgagctcgg tccggaatgt agatcgtgaa ccgcaagcgc
     3061 ggtaaaaagc gtgacgagcg gtgttgccag tccgtcgttc agtccgctct ccacattgag
     3121 tgcctgccgg atacgccctg gcactcgtgg attggtgaag atgggcagtc cgagtgcagc
     3181 gtcggtcgga gcgaggatcg cccccatcaa caacgcgaaa ccaatgccct cgtcacgaaa
     3241 cagaacgtac gccacggcag cacccaggag cacggtcaat ggcagaccaa tcgccaggag
     3301 gcgcgtaggc aacccggcat ccaggcgcac tttcttgaga tccagcgtcg aagcatcggc
     3361 aaacaggatc atcgccaggg tgagctcaag caccaactca gatgttgcgg ccgatgatgg
     3421 aatgtcgagc cagccgagcg cgtccggccc gaccaacgcg ccgaccagga cgagaaacat
     3481 cggcatcgtc acggagtagc gaccaaacca ggccgcgaac accgagaaga acacgacgac
     3541 ggcaaggtag acgacccatt ctcgttcgct cacacatcag ctcctcttcg ttgactctgc
     3601 cgtctaagga actggctaaa gccaggtgaa agactcgcgg gcatgggttg gcgaaatgcc
     3661 gtaatacgtg gtcgctacct
//
LOCUS       DATAOZ010000427         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_436164, whole genome shotgun sequence.
ACCESSION   DATAOZ010000427 DATAOZ010000000
VERSION     DATAOZ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436164"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..755)
                     /gene="lexA"
                     /locus_tag="VHR64_10955"
     CDS             complement(36..755)
                     /gene="lexA"
                     /locus_tag="VHR64_10955"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX3303390.1"
                     /translation="MLTKKQYELLLFIDRHLKQTGFSPSFEEMKDALKLKSKSGIHRL
                     ISALEERGFLGRRRHRARALEVLRVPENLAVHDDDDDAATTKSPAFLPTVIRGDFTSR
                     IPAVRAANDATAIQLPLYGRIAAGLPIEALRDPTTYVDMPMQMLGSGEHYALEVAGDS
                     MVEAGILDGDTVIIRKGDIAETGQIVVALVDDNEVTLKRIRRRGNSIALEPCNVRHET
                     RIFPADRVKVQGRLVALLRRY"
     gene            complement(883..>1545)
                     /locus_tag="VHR64_10960"
     CDS             complement(883..>1545)
                     /locus_tag="VHR64_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX3303391.1"
                     /translation="IAMPGEPIPPGGIVSSNSHALAALVRAGGGEPVVLPVAQDTREA
                     VAAVADAVLGMDMLVTTGGASVGDHDLVIEGLQTRGLVVDFWQIAMRPGKPLLFGRLG
                     AVPVLGLPGNPVSAMVCAILFLLPALSRLSGLPAAPPPTVQAIAGASLRANDHRADHL
                     RSTVSTDSRGRVVATPFTVQDSAMLRRLASADALILRAPNAPALPEGSEITVIRLDSL
                     GI"
BASE COUNT          280 a          493 c          496 g          276 t
ORIGIN      
        1 cgatctgcgg tgtgaagttg gtgttctaac gccggtcagt agcgccgaag cagtgcgacc
       61 agccgacctt gcaccttcac gcggtcggca ggaaagatgc gcgtctcgtg ccggacattg
      121 catggctcga gtgcgatcga attgccgcgg cgtcggatcc gcttaagggt gacctcgtta
      181 tcgtccacca gcgcgacgac gatctggcca gtctccgcta tgtcgccctt gcggatgatg
      241 accgtgtcac cgtcgagaat gccggcttcg accatagagt ctccggcgac ttcgagcgcg
      301 tagtgctcgc cgctgcccag catctgcatc ggcatgtcga cgtaggtcgt cgggtcgcgc
      361 agagcttcga tcggcaagcc cgcggcgatc cttccataca gcggcaactg gatcgcggtg
      421 gcatcattgg cagcgcggac ggctggtatc ctggacgtga agtcgccgcg gatcacggtc
      481 ggcaggaacg cagggctctt cgtcgtggct gcatcgtcgt cgtcatcgtg aactgccagg
      541 ttctccggca cgcgcagtac ctccagggcg cgcgctcggt gccggcggcg accgaggaag
      601 ccgcgttcct caagcgcgga gatcaaccgg tgaatacctg acttcgactt gagcttcagc
      661 gcgtccttca tctcttcgaa gctcggcgag aagccggtct gcttcagatg tcggtcgatg
      721 aacagcagca gttcatactg cttcttggtc agcatgacgc cggcctttcc cctcggagtc
      781 ccgaacgcga agtccggaac aaatcagaaa ctattgatgt tctagatagg ttcgcgccgc
      841 gtcgtcaagt ttttccctga acgagtagcg aaagattcac cgtcaaatac cgagcgaatc
      901 caggcggatc accgttatct cactgccttc aggtagtgct ggcgcgttcg gcgctcgcag
      961 gatcaacgcg tccgcgctcg caaggcggcg cagcattgcc gaatcctgga ccgtgaatgg
     1021 ggtcgctacg actcgccccc gcgaatcggt gctgacagtc gaacggagat ggtcggcgcg
     1081 atgatcgttg gcacgcagcg aggcgccggc gatcgcctga acggtcggcg gcggtgcggc
     1141 cggcagtccg gataggcggc tcagagccgg cagcaggaac aggattgcgc agaccatcgc
     1201 cgagaccggg ttgcccggca gaccgagcac cggcacggca cccagtcgtc cgaacagcag
     1261 cggtttcccc ggtcgcatgg cgatctgcca gaagtcgacg acgaggccgc gggtctgcaa
     1321 tccctcgatc accagatcat ggtcgcccac cgaggcgccg ccggtggtca ccagcatgtc
     1381 catgccgagt accgcatcgg caaccgcggc caccgcctca cgggtgtcct gggcgacggg
     1441 caggacaacc ggctcgccac ctcccgcccg caccagtgcc gccagggcat gcgaattgga
     1501 gctgacgata ccgcctggcg gaatgggctc gccgggcatc gcgat
//
LOCUS       DATAOZ010000428         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6488707, whole genome shotgun sequence.
ACCESSION   DATAOZ010000428 DATAOZ010000000
VERSION     DATAOZ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6488707"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(932..1492)
                     /locus_tag="VHR64_10965"
     CDS             complement(932..1492)
                     /locus_tag="VHR64_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303392.1"
                     /translation="MAAISAETADQIHDMQAFLWIYGRSAHEIYSLVQLAGNGRLVSI
                     GSHYRGVVRWVDWYGDGSLWVLNVALACCTLEFDAASSATAGPLEVLDDRAQAAVVIS
                     GTVTDRLAPAVAAIVDGCELQTGRRPTVISFGACASSGGPYWDSYAVTKGVDQLLAVD
                     AYVPGCPPPPEALSAVLDQVAGRVNA"
     gene            1554..>3087
                     /locus_tag="VHR64_10970"
     CDS             1554..>3087
                     /locus_tag="VHR64_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX3303393.1"
                     /translation="MKHVNTLDRVVIRFAGDSGDGMQLTGDRFTSDSAVFGNDISTLP
                     NFPAEIRAPAGTLPGVSSFQLHFANYDIMTPGDRPDVLVAMNPAALKANIADLPRGGV
                     IIADSADFSKRNLAKVGYDSDPLTDGSLEDYQVHALDLTGMTIEAVKQFGLTRKDATR
                     AKNMFALGLLSWLYQRPTDGTINFLTTKFASKPAIRDANITAFKAGYAFGETTEVFAV
                     RYEVAPAPMPPGKYRQISGNLALAYGLITASRKAGLPMFLGAYPITPASDVLHELSKH
                     KRFGVTTFQAEDEIAGVGAALGASFAGHLGVTTTSGPGVVLKSETIGLAVATELPMII
                     CDIQRAGPSTGMPTKTEQADLLQVMFGRNGEAPLPVLAAQSPADCFPTAIEAVRIAIK
                     YRTPVVILSDGYIANGAEPWLIPDLDSIPAIDPEFASATNATDKAGEPAFLPYLRDAQ
                     TLARPWAIPGTAGLEHRIGGIEKAKDTGAVSYDPANHEEMVRTRQAKINGIVQDIPDL
                     TVD"
BASE COUNT          635 a         1050 c          882 g          520 t
ORIGIN      
        1 cggctcattt gggtgaaacg cccaatacgg aggcggaggt gtgctactcg attactgagt
       61 ctatggatac ttctcgacca tctcctcaac cagcgatccc gagctcaagc cgggttgcgt
      121 caggtcagcg acccattcca gcgcgtcgta cggacactcc tcaacgcaga tgccgcaata
      181 catacacagc gaccagtcga tggcgaaacg atcaaggaca ttcctcgtgc gatctcgggc
      241 tcccggccgt agccccgctt cgagctcggt gtgcgaatta atgctgatgc accagctcgg
      301 gcattcccgc gcacagatca tgcacgaggt gcaagccggt tcgatcaact ggatccagcg
      361 atgcgaggtc tcagtcgaac tcacgtacgc ctccgccgca ctctgccgcc aacagccgaa
      421 tcggccacct cggctgggtc agccggcggc tgcgatgcat cgcgatcacc ccataccgcg
      481 ggctcaggta cgccgggagg caccattcgc cggcgacccg gcgacacatc agattctccc
      541 ggctccttag ctccaggcca atccatcccg gtacgcgccg ccagcacctc atccttacgc
      601 agcggggtcc cttcgaactc ggggttgagt aacagccgac ggcgttcccc gcctaggaac
      661 tcaacaccaa acagttcggc cacctctcgc tcatgccaag cggcaccggc aaagagacca
      721 gacaccgtgt cgattcgcgg agcatctcga ggcaatgagc agctcagcat tcgtggcgcg
      781 gctccttggg cgaggttgcg taggaccagc acgatccgaa agcagtcccg ccggccaatc
      841 tcatcaacgc agcccagcca gtcgaagtag ttgaaaccat ccgccttcgc agattcgacc
      901 gcggccaccc actcctcagt caacagttca gtcacgcgtt cacccgcccg gcaacctgat
      961 ccagcaccgc cgacaacgct tccgggggag gagggcaacc cggaacgtag gcatcaacag
     1021 ccagcagctg atcgactccc ttggtcaccg cgtaggagtc ccagtaggga ccgcctgaag
     1081 aggcgcatgc cccgaacgag atcacagtcg gtcggcgtcc cgtttgcaac tcgcatccgt
     1141 cgactattgc cgccacagcc ggcgccaacc gatcggtcac ggtgccagag atcaccaccg
     1201 ccgcctgggc ccgatcatca agaacctcga gcggccccgc tgtggcagac gaggcagcgt
     1261 caaattccaa cgtgcagcat gccaacgcga cattcagcac ccacagggag ccgtcgccgt
     1321 accagtcgac ccaccgcacg actccacggt aatgcgatcc gatggagacc aagcggccgt
     1381 tcccagctaa ttgaactaac gaatagattt catgggctga acgtccgtaa atccacagaa
     1441 acgcctgcat atcatggatt tgatcggcag tctcagcgga gatcgcagcc acgaaccccc
     1501 ctgaccccaa agcagcggat gaggcacgcc gcaggaagaa ggcacgcaga gtagtgaagc
     1561 acgtcaatac cctcgaccgg gtcgtgatcc ggttcgccgg ggactcgggt gatggcatgc
     1621 aactcaccgg cgatcggttc acctcggact cggcagtatt cggcaacgac atctcaacgc
     1681 tgcctaactt cccggccgaa atccgagcac ccgccggcac ccttccgggc gtgtccagct
     1741 tccagctgca ctttgccaat tacgacatca tgacccccgg cgaccggccg gatgtgctgg
     1801 ttgcgatgaa tccggcggcc cttaaggcca acatcgccga tctgccacgc ggcggcgtga
     1861 tcattgccga ttccgctgac ttctccaagc gcaacctggc gaaggttggc tatgacagcg
     1921 atccgctcac cgacggctcg ctcgaggact atcaagtcca tgccctggat ctgacgggca
     1981 tgaccatcga ggcggtgaag cagttcggcc tcacccgcaa ggacgccacg cgcgccaaga
     2041 acatgtttgc gctcggactg ctgtcctggc tgtatcagcg gccgaccgac ggcacgatca
     2101 acttcttgac caccaagttc gcatccaagc ccgcaatccg cgacgcgaac atcaccgcct
     2161 ttaaggcggg atacgcattc ggcgagacga ccgaggtctt cgcggttcgg tacgaggtgg
     2221 cgccggcgcc catgccgccg ggcaagtacc gccagatctc gggcaatctc gcgctggcgt
     2281 acggactgat caccgcgtcc aggaaggccg gtctgccgat gttcctgggt gcttacccga
     2341 tcactccggc atcggatgtg ttgcatgaac tgagtaagca caagcgattc ggtgttacca
     2401 cattccaggc tgaggatgag atcgccggtg tcggcgctgc gctgggcgcc agcttcgccg
     2461 ggcatctcgg cgttacgacc acctccggcc cgggcgttgt cctgaagtca gaaaccatcg
     2521 ggctcgccgt tgcgaccgag ctgccgatga tcatttgcga catccaacgc gccgggccga
     2581 gcacgggcat gccaaccaag actgagcagg ccgacttgtt gcaggtgatg ttcggccgca
     2641 acggtgaggc accgctccct gtgctggcgg cgcaatctcc cgccgactgc ttcccgacgg
     2701 cgatcgaggc ggtacggatc gcgatcaagt accggacccc ggtcgtgatc ttgtcggacg
     2761 gctacatcgc caatggcgct gagccatggc tgatcccaga cttggattcc atcccggcga
     2821 tcgatcccga gttcgcttcc gcgaccaacg cgaccgacaa ggctggcgag cccgccttcc
     2881 tgccgtactt gcgcgatgcg caaacgcttg cccggccctg ggcgattcct ggaaccgctg
     2941 ggctggagca ccgcatcggc ggcatcgaga aagccaagga caccggcgcg gtttcctacg
     3001 acccggccaa ccacgaggag atggttcgta cccggcaggc caagatcaac gggatcgtcc
     3061 aagacattcc tgacctgacc gtcgatg
//
LOCUS       DATAOZ010000429         5113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_178905, whole genome shotgun sequence.
ACCESSION   DATAOZ010000429 DATAOZ010000000
VERSION     DATAOZ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178905"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VHR64_10975"
     CDS             complement(<1..433)
                     /locus_tag="VHR64_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX3303394.1"
                     /translation="MNTPLDGITVIDMSRALAGPYAAMTLGDFGANVIKIEDPRGGDA
                     TRDYPPFWNGQSTYFLSANRNKRSLTLNLEHPEAREIVRRLVAEADILIESFRTGAME
                     AWGLGWEELHALNPRLIYCAVSAVGRDGPDKDRAGVDLLMQA"
     gene            691..849
                     /locus_tag="VHR64_10980"
     CDS             691..849
                     /locus_tag="VHR64_10980"
                     /inference="COORDINATES: protein motif:HMM:NF039193.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX3303395.1"
                     /translation="MAAVEAYCVKCREKREMADAEEVTMKNGRKAMQGKCPVCGTKLF
                     RILGGKSK"
     gene            complement(870..1607)
                     /locus_tag="VHR64_10985"
     CDS             complement(870..1607)
                     /locus_tag="VHR64_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3303396.1"
                     /translation="MTAPFHRPHRTAARAHRRETLTMERVVETALALVDRDGLDRLSM
                     RRLGAELGVDPMAVYHYIPNKAALLDGLTEAVMNELGIAPPRKPDEDVTEWFVYSFHS
                     FWEVLRTHPNVLPVMATRPITGDSGLRSAERVLEELHGIGLPPDDAMAAMLALTTMTV
                     AMALAEAGRDPQNMDPSVLAKVQDCYRSLPPAEFPLFLAGISQPQAKDWGRIFDISIR
                     MFVAGLFATYAPGTQKPAAQVETVTAG"
     gene            complement(2129..3352)
                     /locus_tag="VHR64_10990"
     CDS             complement(2129..3352)
                     /locus_tag="VHR64_10990"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEX3303397.1"
                     /translation="MTDLALIADDLTGAADSGVPFAQAGFTTAIPFDQIRREAADVLC
                     LSTDSRDVSVAEAERRVRQALIAVFGRDSAHRTPWIYKKMDSALRGHPAEELLVTMRL
                     AGVTRAVVAPAIPSQARTTVGGIALDHGMPLAQTPIGQEHGRDNLARLFQRSVRVPVR
                     LIDLPTVRGPDRALRNALSLPGSWIAIADSQTEADLDRIAVTGVAAGIRLFCGAAGLT
                     GVLARRLPIHQVNRPPAVLASDRPVLVVAGSRHQVTARQIEVAVAAGAEIIRLDQCAI
                     DDETPDLSGYQVRIVAALEAGRTTIVTTVGLGESRLQGAVVSERLAAAAINSEVLARV
                     GGLVLTGGDVAAAVCRALGVRTIWLRGEVLPAIPWATFEGERVAGLPVVTKAGSFGPP
                     EAIGAAIQFLRGLAP"
     gene            complement(3453..4466)
                     /gene="pdxA"
                     /locus_tag="VHR64_10995"
     CDS             complement(3453..4466)
                     /gene="pdxA"
                     /locus_tag="VHR64_10995"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX3303398.1"
                     /translation="MADRDSRPLLAITVGDPAGIGPEIILKALVHADVFERCRPLVVG
                     DRRILERAAPWVDQPAPSFEPITNPQDGAYRSGTVPILDLGDADPGEIPVGVESAAGG
                     RAAVEAVFRACDLTMAGETDAVVTAPLNKAAMHMAGFNYPGHTELLAERTGAGKVTML
                     LTGPNLRVVHVSMHVGLAEAIARVTTERVLDTIRLADSACRSLGIARPRIAVAGLNPH
                     ASEGGLFGFEEAMAIIPAIAAARTEGLDVSDPQPPDTVFLRATKGVYDIVVAMYHDQG
                     HIPMKLLAFDSGVNVSIGLPIIRTSVDHGTAFDIAGTGKAREESLIAAIDVAIQMVAA
                     REG"
     gene            complement(4773..>5113)
                     /locus_tag="VHR64_11000"
     CDS             complement(4773..>5113)
                     /locus_tag="VHR64_11000"
                     /inference="COORDINATES: protein motif:HMM:NF025020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEX3303399.1"
                     /translation="GDRLMLATDQALDLAGVPVVRDGLWLGDRKPGRFEPSHALALAI
                     RAEVARNRLDLDVDATRRWIAGEPVNAAGNAGWVLVTVDDFALGWGKRSGTTVKNHYP
                     KGLRRAVVNR"
BASE COUNT          948 a         1795 c         1570 g          800 t
ORIGIN      
        1 aggcctgcat caacagatcg accccggccc ggtctttgtc cggaccgtcc cgaccgacgg
       61 ctgagaccgc gcagtagatc agccgcgggt tgagcgcgtg caactcctcc cagccgagcc
      121 cccacgcctc catcgccccc gtccggaagc tctcgatcag gatgtccgcc tccgcgacga
      181 gccggcggac gatctcccgc gcctccgggt gctccagatt gagcgtcagg ctgcgcttgt
      241 tgcggttcgc gctcaggaag taggtgcttt gcccgttcca gaacggcgga tagtcacgcg
      301 tggcatctcc gccccgcgga tcctcgatct tgatcacatt cgcgccgaag tcgccaagcg
      361 tcattgccgc atagggtccg gccaatgccc ggctcatgtc aattacggtt ataccgtcaa
      421 gtggcgtatt cacaggagaa ctgatcgctc cgcttccagg ttcagccgga ttccgatccg
      481 ggaatcatgc ctgatgtggg cgcattcggt cacggggcgt agcgtggatg gcggaagaag
      541 agcgtacgta cgcgcagatt ttcttgcgcg cagccttagt taccagtact atcgcggctc
      601 agaaggcgtc acgcaggctg gaagaccggc tacggatcga tggtgatctg cgagagaaca
      661 cgtggcgttc acggggagga catacatcac atggcagcag tcgaagccta ttgtgtcaag
      721 tgtcgtgaga agcgcgagat ggcggatgcc gaggaagtga ccatgaagaa tggtcgcaag
      781 gcgatgcagg gcaagtgccc ggtttgcggc accaaactct tccgcatcct gggcggcaag
      841 agcaagtaac tccgcccgcc aaagcgattt caaccggcgg tgacggtttc tacctgagcc
      901 gccggttttt gcgtgcccgg cgcatacgtc gcaaagagac cggccacaaa catacggatg
      961 ctgatgtcga agatccgacc ccagtccttg gcctgcggct ggctaatccc cgcgaggaac
     1021 aacggaaact ccgccggcgg caaagaccgg tagcaatcct gcaccttggc cagcaccgat
     1081 gggtccatgt tttgcggatc ccgtccggcc tcggcgagcg ccatggcgac tgtcatcgtg
     1141 gtcagcgcca gcatcgccgc catcgcgtca tccggcggca acccgatccc atgcagctct
     1201 tcgaggaccc gctcggccga gcgcagcccg gaatcgccgg taatcggccg ggtcgccatc
     1261 acgggcaaca cgttcgggtg cgtccgtagc acctcccaga agctgtggaa cgagtagacg
     1321 aaccactcgg tcacgtcctc gtccggcttg cgcggcggag cgatgccgag ctcgttcatg
     1381 accgcttcgg tcagcccatc gagcaaggcg gccttgttcg ggatgtagtg gtaaacggcc
     1441 attggatcga cgccaagctc agcgccaagt cggcgcatac tcagccggtc cagaccatcg
     1501 cggtcgacga gggcgagcgc cgtctcgacg acgcgctcca tagtcagggt ctcgcgccga
     1561 tgcgctcggg cggcagttcg atgtgggcgg tggaatgggg cagtcacgac acgatcctga
     1621 acgcctcgat cagatatggc caagtctaca ccgtagaccc accgaccgtg ccacccaaat
     1681 cacccaatct ccgccgtccc atcgcaatac gacccctcca gcgcgatgca tgtcgggcgg
     1741 tctcccctct ctgaccggcg ttgaaccacc gagctacatc ccataaggcc gctaaagccg
     1801 gccggataga gctatcccaa tgcggcgcgg gtgatgctct cttccctgga ggcgagagtc
     1861 ggagcgggga tgtgccccgc gatgacgcgc agcggcacga cggtgcaaag ggtagaccgg
     1921 gtcgatgcac gcaccccgga cccggccacc gccccgggct taaggccggg gctgagagag
     1981 gataaggcgg ctaaagccga ctctcttaga gtcccgaagg gccttatcgt gactcagccc
     2041 gaacctttag cttcgggcgg tgccgggaaa cccgtccaat agagaaacga caagtgaccg
     2101 gacaccgaag cggccgagtg ccggtgcgct acggcgcgag accgcgcaga aactggattg
     2161 ccgccccgat cgcttccggc ggcccgaagc tccccgcctt cgtcacgacc ggcaacccgg
     2221 caacccgctc accctcgaac gtcgcccacg ggatcgccgg caacacctca ccccgcagcc
     2281 agatcgtccg cacgcccagt gcccggcaca ccgccgccgc gacatcccca ccggtcagca
     2341 ccagtccccc gacccgcgcc agcacctcgg agttgatcgc cgcggccgcc aatcgctctg
     2401 acacgacggc accctgcaat cgcgactccc caagcccgac cgtcgtcacg atcgtcgtcc
     2461 gaccggcctc gagcgccgcc acgatccgga cctggtagcc gctcaggtcc ggcgtctcgt
     2521 cgtcaatagc gcactggtca agccggatga tctccgcacc tgccgcaacc gcgacctcaa
     2581 tctgccgcgc cgtaacctga tgacggctcc cggccaccac cagcaccggc cggtcgctcg
     2641 ccaacaccgc cggtgggcga ttgacctggt ggatcggcaa gcgccgggcc agtacgccgg
     2701 tcagcccggc cgcgccgcaa aacaggcgaa taccggccgc gaccccagtc accgcaatcc
     2761 ggtccagatc cgcctcggtc tgggaatcgg cgatcgcgat ccacgacccc ggcaacgaca
     2821 gcgcattccg cagcgcccgg tccggaccgc gcaccgtcgg caaatcaatc agccggaccg
     2881 gcacccgcac tgagcgctgg aaaaggcggg ccagattgtc gcgcccgtgt tcctgcccga
     2941 tcggcgtctg ggcgagtggc atgccatgat cgagcgcaat cccgccgacc gtcgtccgcg
     3001 cctgactcgg gatcgccggc gccaccactg ctcgcgtcac gccggccaga cgcatcgtga
     3061 ccagcagctc ctcggccgga tgaccgcgca gggccgaatc catcttcttg tagatccacg
     3121 gcgttcgatg cgcgctatcc cggccaaaca cggcgatcaa cgcctgccgc acccgccgct
     3181 cggcctccgc gaccgagaca tcgcggctgt cggtcgaaag acagaggaca tcggcggcct
     3241 cgcgccggat ctggtcgaaa gggatcgcgg tcgtgaatcc ggcctgagcg aacggcacgc
     3301 ccgaatcggc tgcgccggtc aagtcgtccg cgatcagcgc cagatcagtc atagccgaac
     3361 cggtctgtgg ttgatggtgg ttggttgttg gtggagggaa cgagaaactg agtcggcgta
     3421 ccctccctcc aaccaccaat cacgaaccac catcaaccct ctcgagcggc caccatctgg
     3481 atcgcgacat cgatcgccgc gatcaggctc tcctcgcgcg ccttccccgt gccggcgata
     3541 tcaaaggccg tcccgtgatc gaccgacgtg cggatgatcg gcaagccgat gctgacattg
     3601 accccgctat cgaacgccag cagcttcatc gggatgtggc cttgatcgtg gtacatcgcg
     3661 accacgatgt catagacacc cttggtcgcg cgcaggaaaa cggtatccgg cggctgtgga
     3721 tcggagacat ccagcccctc ggtccgggcc gccgcgattg cgggaatgat cgccatcgcc
     3781 tcctcgaagc cgaacaatcc accctcgctt gcatgcggat tgagcccggc gactgctatc
     3841 cgcggccgcg cgatcccgag cgagcgacac gctgaatcgg caaggcggat cgtgtccagc
     3901 acgcgctccg tcgtgacccg cgcgatcgcc tcggccagcc cgacatgcat cgagacatgg
     3961 acgacgcgca ggttcggacc ggtcagcagc atcgtgactt tgcccgcgcc ggtccgctcg
     4021 gccagcaact cggtgtgccc gggatagttg aagccggcca tgtgcatcgc cgccttgttc
     4081 agcggcgccg tcacgaccgc atcggtctcg ccggccatgg tcaggtcgca ggcccggaag
     4141 accgcctcga ctgccgcccg gccaccggcg gccgactcca cgcccaccgg gatttcgccc
     4201 ggatcggcat cgccgaggtc gaggatcgga accgtgcccg accggtacgc gccatcctgc
     4261 gggttcgtga tcggctcgaa cgagggtgcc ggctggtcga cccacggcgc ggcccgctcc
     4321 aggatccgcc gatcgccgac caccagcggc cggcagcgct cgaagacgtc cgcatggacc
     4381 agcgccttga ggatgatctc cggcccaatc ccggccggat cgccaacggt aatcgccagc
     4441 agcggcctcg aatcccggtc agccatcgcg catcaacctc cccgtcgaat acgggtagcg
     4501 tacgggaaat gaaggcgagc agtcaggagg cgagcagggg agcagttggg gcgcggagac
     4561 gggagggcgc gaaggcgcgg cgtaggaggg gtgcgaaggc ggaagggcgc ggaggtgtgg
     4621 tgttgtaggg gcagagggca tctgcccgcc ggggacgtcc aacgttcagt tcacctatac
     4681 acaccaatcg gtttcaaccg aatcccgtta ttttgacggg gaatccgatc cccggcgggc
     4741 cgcatatccg gagtgagtcg acgcgtccgc cgttaccgat tgacgaccgc gcgacgcaaa
     4801 cctttcgggt agtggttctt gaccgtcgtg ccgctgcgtt ttccccagcc aagcgcaaag
     4861 tcgtcgactg tgaccagcac ccatcccgcg tttcccgccg cattgaccgg ctccccggca
     4921 atccaccgtc gcgtcgcatc gacatcgagg tccaaccggt ttcgcgcgac ctcggccctg
     4981 atcgccaatg ccagggcatg tgacggctcg aaccgccccg gcttccgatc ccccagccat
     5041 agcccatccc gcaccaccgg cacgcccgcc agatccagag cctgatccgt cgccagcatc
     5101 aatcggtcgc cac
//
LOCUS       DATAOZ010000430         4614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8067362, whole genome shotgun sequence.
ACCESSION   DATAOZ010000430 DATAOZ010000000
VERSION     DATAOZ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8067362"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VHR64_11005"
     CDS             <1..349
                     /locus_tag="VHR64_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303400.1"
                     /translation="QDADQDANWFCHGDSDTYQDANNDGYGDEDADGNQYADENQHAN
                     RYSHADGDWYTNRYSHADRYSDTDAYANQHRNTDKDQHTDVGKRSRRAERSALLSSCP
                     LPVPCDSSPGDWT"
     gene            complement(346..1563)
                     /locus_tag="VHR64_11010"
     CDS             complement(346..1563)
                     /locus_tag="VHR64_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3303401.1"
                     /translation="MDASPVSRRAAAFHDLSSFTVIRDFYFNSPYTERRNEPGIIDFT
                     FGNPHDMPMPEYVDALREATTPQDKDWFAYKDYVPAAQEAAAASLERLLRMPFAPEDV
                     LLTTGGFAALAISLKAAADPGDEVIYSLPPWFLYEGLCAEAGLNPVRVTIDLTNFDLD
                     VDAIAAAITPRTRVVMVNTPNNPTGRIYPPETLQQLAAVLDAASVRNGRRIYLISDEP
                     YNRIVFDGARFRSPAEFYPYTFIAYSYGKTHLSPGQRIGYIALPPTMPDREEIRMAIM
                     QLQILAGWVFPNAIMQYALPRLEQFTIDIDRLQRRRDRLVEALTDMGYSVHRPEGTFY
                     LWPRSPIGDDAAFARQLTEHDVFVLPGHMFETPGFFRICLTANDDMIERSLPGFAAAI
                     KQVQAEQPAFIST"
     gene            complement(1712..2485)
                     /locus_tag="VHR64_11015"
     CDS             complement(1712..2485)
                     /locus_tag="VHR64_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303402.1"
                     /translation="MPLAADTSVGWTVGSEIDGVRKSGVAVEVGGFAVDVGNGGIGVL
                     VGIGMVGIGMVGNGTVAVGGTGVLVGGTGVFVGGTGVCVGGGMVGSGMVGDGLGVFVG
                     LGVCVGGGGFGVFVGGGGSGVLVGGGGSGVFVGGGGGGGSGVFVGGGGGGGSGVSVGG
                     GGGGSGVSVGGGGGGSVGSMVGKGSICAMVMGDADGRTGARVASVIRITKNVRERILA
                     MGTAFAGAVTGQAGLSGNGFVDTGDRKRGLLPEAPHPIV"
     gene            2466..3209
                     /locus_tag="VHR64_11020"
     CDS             2466..3209
                     /locus_tag="VHR64_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303403.1"
                     /translation="MSAASGKIDKSGYGATVRCTYRVKTDTTACRFNINVAEGAFPVN
                     YAVLTSQMCTEVMDGSITYVNPDPVTGATGYAFAGRNNRMVMVGKVTVRGNARYILSV
                     GGNVFGITGPGLACGISKTEGTMIIASRFVCTVAADDPRASAGLMFDQAVNAPFQAPN
                     GASDCHAVDDGEASFTLDYVDEVPSVGGIPTSPNGRVRFRHVVAGQYRLIDGESGTQS
                     TSFRVPGNGLTIFHVIEFVPETGTAEKSP"
     gene            3311..4348
                     /locus_tag="VHR64_11025"
     CDS             3311..4348
                     /locus_tag="VHR64_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303404.1"
                     /translation="MRNRRSLPYLVALLALLAALIGPVASPVHADNAGHNHGDDQKHD
                     GGHKKDKPPPDPTEAPTVEVTLPPEPTETATTTAEPTDTVTPTPELTGTTAPPVNEPA
                     APPGPFIDPGTYQIAVTCKYYKEKDATACRFAVEPGFGGIPANHAILTSALCTEVMSG
                     EVGYVDWDAATGTQGYWLDQANDRMVLVGQVRVKGHAHYAVQVGSSDFKLSGPGLSCG
                     VSKSGGTQIIVSKYICSVPADDPRAGESAGFVQYQNVPFEPPAGADRCRHVAEGETSF
                     TLDYVDGVPSAGGLPTRANGRVRFTGVREGRYRLVERTSHTRSDVFTPSGSGLLLFRV
                     VEFVPDTRAGR"
     gene            complement(4355..>4614)
                     /locus_tag="VHR64_11030"
     CDS             complement(4355..>4614)
                     /locus_tag="VHR64_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX3303405.1"
                     /translation="VPGHPNDYPYDVISISLDLGMAKIPGLELRMRPWIQDFSLPGMK
                     EYGPEQIRDEIDAAEDSGSSGWLVWNVNADYVHGAYKKADE"
BASE COUNT          902 a         1564 c         1404 g          744 t
ORIGIN      
        1 ccaagacgcc gaccaagacg ccaactggtt ctgccacggc gactcggaca cctaccagga
       61 cgccaacaac gacggctacg gtgacgaaga cgccgacggt aaccaatacg ccgacgaaaa
      121 ccaacacgcc aaccgctact cacacgccga cggcgactgg tacaccaacc gctactcaca
      181 cgccgaccgg tactccgaca ccgacgcata cgccaaccag caccgcaaca ccgacaaaga
      241 ccaacacacc gacgtaggca agcgcagcag gagagcggag agatccgctc tcctctcttc
      301 ctgcccgctt cccgtgccgt gtgactcgag tccgggtgat tggacctagg ttgagataaa
      361 tgccggttgc tcggcttgga cctgcttgat ggcggcggcg aagccgggca ggctgcgctc
      421 aatcatgtcg tcgttggcgg tcaggcagat ccggaaaaag cctggcgtct cgaacatgtg
      481 ccccggcagc acgaagacat catgctcggt caattgccgc gcgaaggccg catcgtcgcc
      541 gattggcgag cgcggccaga ggtagaacgt gccctccggc cgatggacgg agtaccccat
      601 gtcggtgagc gcctcgacga gacggtcgcg gcggcgctga agccggtcga tgtcgattgt
      661 gaactgctca agccggggca gggcgtactg catgatcgcg ttcgggaaga cccagccggc
      721 caagatctgc agctgcatga tcgccatgcg gatctcctcg cggtccggca tggtcggcgg
      781 caaggcgatg tagccgatgc gctggcccgg cgagaggtgc gtcttcccgt acgagtaggc
      841 aatgaaggta tacgggtaga actcggccgg gctgcggaag cgggccccat cgaagacgat
      901 ccggttgtac ggctcgtcgg agatcaggta aatgcgccgc ccattgcgga ccgacgccgc
      961 gtcgagcacc gcggccaatt gctgcagggt ttcgggcggg tagatccggc cggtcgggtt
     1021 attgggcgta ttgaccatca cgacgcgggt ccgcggggta atggccgcgg caatcgcgtc
     1081 gacatcgagg tcgaaattgg tcaagtcgat cgtgacccgc accggattca gcccggcttc
     1141 ggcgcagagc ccttcgtaca ggaaccaggg cggcaggctg tagatcacct cgtcgcccgg
     1201 atcggcagca gccttcagcg agattgcgag cgcggcgaag ccaccggtgg tcaacaggac
     1261 gtcttccggc gcgaagggca tccggagcaa ccgctcgagt gatgcggcgg ctgcctcctg
     1321 cgcggcagga acatagtcct tgtacgcaaa ccagtcctta tcctgcggcg ttgtcgcctc
     1381 gcgcagggcg tcgacatatt cgggcatcgg catgtcgtgc ggattgccaa aggtgaagtc
     1441 gatgatgccc ggttcgttcc gccgctccgt gtacggtgag ttgaagtaga agtcgcgaat
     1501 caccgtgaaa gacgagagat cgtggaatgc ggcagcacgg cgggagacgg gactggcgtc
     1561 cacagaggca cctccggata gttcgcgttg cgaaacgacc tccccagact atcacccggg
     1621 gcaatacacc cggggccgat tcgggtcgct caaagtggcg gtgttggcca cgacactgac
     1681 ggccattgac atattcgcta tgctaaaagt cctacacgat gggatgaggc gcctccggca
     1741 gcagaccgcg cttgcggtcc cctgtatcca cgaaaccgtt gccggacagt cctgcttgac
     1801 ccgtcaccgc acccgcaaag gcggtaccca tggccaagat gcgctccctg acgttcttcg
     1861 taatcctgat tacgctggcg accctggcgc ccgttctccc gtcagcgtcc cccatcacca
     1921 tggcacaaat cgaccccttg ccgaccatcg aaccgaccga acctccaccg ccaccgccga
     1981 cggacacccc ggagccacca ccgcccccac cgacggatac tcctgaacca ccgccgccac
     2041 cgcctccgac gaacacgccc gagccacccc ctccgccccc gccgacgaac acgcccgagc
     2101 ctccgcctcc gactaataca cctgagcctc ctccgccaac gaacacaccg aagccgccgc
     2161 caccgacgca cacacccaaa ccgacgaaca cgcccaagcc atcaccgacc attccgctgc
     2221 cgaccatccc gccaccgacg cacactcctg tgccgccgac gaatacacct gtgccaccta
     2281 cgagtacgcc tgttccgccg actgcgaccg tcccgttgcc aaccatcccg attccaacca
     2341 ttcctatccc aacgagaacg ccgattccgc catttccgac gtcgacagca aatccaccta
     2401 cctcgaccgc gacgccggac ttcctgacgc cgtctatttc cgagccgacg gtccagccga
     2461 ccgaggtgtc agcggcaagc ggcaagatcg ataagtccgg ctacggcgcg accgttcgct
     2521 gcacctaccg ggtcaaaacc gacactaccg cctgtcgctt caatatcaac gttgccgagg
     2581 gcgcgtttcc ggtcaactac gcggtgctga cgagccagat gtgtaccgag gtcatggacg
     2641 gctcgatcac ctacgtcaat ccggatccgg taacgggcgc cactggctac gccttcgccg
     2701 ggcggaacaa ccgcatggtg atggtcggca aggtaaccgt gagggggaac gcccgctaca
     2761 tcctgagcgt cggcggaaat gtcttcggca tcaccggacc gggtctggcc tgcggcatct
     2821 caaagacgga gggcacgatg atcatcgcct cgcgcttcgt ctgcacggtc gcggccgacg
     2881 atccgcgcgc atcggcgggg ctgatgtttg atcaggcggt caacgcgccc ttccaggctc
     2941 cgaatggcgc gagcgattgc cacgcggtcg acgacggaga ggccagcttc accctggact
     3001 acgttgacga ggtgccgtcc gtcggcggaa ttccgacgag tcccaacggc cgggtccgat
     3061 tcaggcacgt tgtggcggga caataccggt tgattgatgg cgaatcgggc acacaatcga
     3121 cgtcgttccg cgtgcccggg aacggcttga cgatcttcca cgtgatcgaa ttcgtcccgg
     3181 agacgggaac ggccgaaaag agcccatgac gcgatagcga tcccggccca ggggtgagga
     3241 atctccctgg gccgggcgta ttcgggcggg ccgctctgtc gatggaaacc cgcggttctg
     3301 gacaatgctc atgcgcaacc gacgctcctt gccatatctc gtcgctctcc tcgcattgct
     3361 tgccgccctg atcggtccgg tcgcatcgcc ggtccacgcg gacaatgcgg ggcacaatca
     3421 cggagacgac cagaaacacg acggaggcca caagaaggac aagccgccgc cggatccgac
     3481 ggaggcgccg acggtcgagg tcaccctgcc tccggagccc actgagacag caacgacgac
     3541 cgcggaacca actgacaccg tcacaccgac accggaacta accgggacaa cggcgccgcc
     3601 tgtgaacgag cccgctgctc cgcccggtcc gttcatcgac ccaggcacct accagattgc
     3661 ggtaacgtgc aagtattaca aggagaagga cgccacggcg tgccgattcg cggtcgaacc
     3721 gggtttcggc ggaatccccg ccaatcacgc gattctgacc agcgcgctct gtaccgaagt
     3781 catgagcggc gaggtcggct acgtcgattg ggacgcggcg accggaacgc agggctactg
     3841 gctcgatcag gccaacgacc gcatggtgct cgtcggccag gtcagggtca agggacacgc
     3901 ccattacgcg gtccaggtcg ggtcgtccga cttcaagcta tccggtcccg ggctctcgtg
     3961 cggagtctcg aagagcggcg gcacgcagat catcgtctca aagtacatct gctcggtccc
     4021 ggccgacgac ccgcgggccg gcgaatcggc cggattcgtc cagtaccaga atgtcccgtt
     4081 tgagccgccc gccggcgcgg atcgctgtcg ccatgtcgct gagggggaga cgagcttcac
     4141 cctggattac gtcgacggtg taccgtcggc cggcggtttg cccacgcgtg ccaatggccg
     4201 cgtccgcttc accggggtca gggagggccg gtaccggctc gtcgagcgca cctcgcacac
     4261 gcgatccgac gtgtttaccc catcggggag cggcctgttg cttttccggg tcgtcgagtt
     4321 cgtcccggac acccgcgccg gccgctgagc cgcgttactc gtcggccttc ttgtaggcgc
     4381 cgtggacgta gtcggcgttg acgttccaga cgagccaacc gctggaaccg gagtcttcgg
     4441 ccgcgtctat ttcgtcccgg atctgctccg gcccgtactc cttcatgcca ggcaacgaga
     4501 agtcctggat ccacgggcgc attcgcagct cgagtcccgg gatcttggcc atgccgaggt
     4561 cgaggctgat cgaaatcacg tcgtacgggt aatcattcgg gtggcccgga acgg
//
LOCUS       DATAOZ010000431         3757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6490021, whole genome shotgun sequence.
ACCESSION   DATAOZ010000431 DATAOZ010000000
VERSION     DATAOZ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6490021"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..2230)
                     /locus_tag="VHR64_11035"
     CDS             complement(164..2230)
                     /locus_tag="VHR64_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX3303406.1"
                     /translation="MTMHPKIADRHLSRQACIYIRQSTLAQVRFNQESTDRQYNLVNK
                     ARSLGWSQEQIRVLDRDLGKSGSAASTRTDFKTLVGDVAMGHVGAIFSLEASRLARSN
                     QDWHRLLELCAITGTLVIDEDGVYCPAEFNDGLVLGMKGTFAQAELHIIRARLHGGKL
                     NKASRGDLRFALPVGLVFDEDKIVLDPDREVQGAVRTVFELFEREGTAYSVVRRFQEL
                     GLRFPRRAYGGAWNGKLLWGRLTHSRVLGLLANPSYAGVYVFGRYQSSKQVGPSGEIA
                     TRSRPVPQDAWRVMIRDHHEGYIDWDRYEANRLRLAGNRTNGEVLSGPAREGLCLLQG
                     MLLCGACGRRLGIRYTGNGGVYPIYQCIWKHREALSPRACLSVTAAPLDQAITGRLVG
                     AITPATIDLALAALTSLEERDREIGAQWRMRIERARYDVDLADRRYEAVDPANRLIAA
                     TLEQRWNDAMRRLQELEAELAAFERQTMRAVTAEQKRQILKLAGDLPRLWAASTTTPR
                     DRKRILRLLVRDITVTKGPEPKIISLHVRWQGGASETLELQMPPNRAEAVRYSEAFVA
                     RIRQLAVNHHDDEIIRLLRAEGHQSSTGKPLTLSMIKWLRYKHRISAPRPPADTLNVR
                     QVRDRYGVSLWVVHYWIGRGVITAQQRKPNAPYEISIDEASDQLLWEWIANSVHLHPS
                     SQTQAA"
     gene            complement(2349..2690)
                     /locus_tag="VHR64_11040"
     CDS             complement(2349..2690)
                     /locus_tag="VHR64_11040"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HEX3303407.1"
                     /translation="MNIRDLPATALRKRRRNLVRDLPPIEQLLRGSLTETYKRCGRLN
                     CHCASGPGHGPKRYLVISQPGGRPRRDYVPNATHEHVASLIGNFNRLREALNEICAIN
                     TELLRRREDLG"
     gene            complement(2733..3008)
                     /locus_tag="VHR64_11045"
     CDS             complement(2733..3008)
                     /locus_tag="VHR64_11045"
                     /inference="COORDINATES: protein motif:HMM:NF036442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y4bD/Y4pK family protein"
                     /protein_id="HEX3303408.1"
                     /translation="MGSAEVVHPFHPLRGQRFVVLKVRTVSGIETLSLRHTDLGSFAM
                     PRDWTDWAPPGSQAGSGDQRLLVDTFGLIALAELFAALRSGDLEVDR"
     gene            3044..>3757
                     /locus_tag="VHR64_11050"
     CDS             3044..>3757
                     /locus_tag="VHR64_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEX3303409.1"
                     /translation="MPTVTDRVAQEVVKRYLEPVLELIFHEDSYGYRPGRSAIDAVRK
                     ARQRCWRYDWVLDVDVRSYFDSIDWELLLKAVRKHTDCRWVLLYIERWLKAPVQLEDG
                     SVEPRTAGTPQGGVISPCLANLFLHYVFDMWMVRNFPHIPFERYADDIICHCRSAEEA
                     RALWGALEARVAACRLALHQQKTKLVYCKDANRRGDHPIQSFDFLGYEFRPRSAIWHG
                     RQLGVSFLPAISPKALKAIR"
BASE COUNT          698 a         1121 c         1221 g          717 t
ORIGIN      
        1 cgtcaaacaa ttgccggggg ttctcaaagc gcgctggccg gagatcgaac gacaactgct
       61 ccaggggcgc taccagccgc aaccggtgcg tcgggtgcaa atcccgaagc cggccggcgg
      121 gacgcgcgac ctcggcattc cgacgtggca ggcataatgc acctcatgca gcttgcgttt
      181 gggatgaggg gtgaagatgt accgagttgg caatccattc ccagagcaac tggtctgacg
      241 cttcgtcgat cgatatctcg tagggcgcat tcggcttgcg ctgctgcgcg gtgatgacgc
      301 cacgaccaat ccagtagtga acgacccaga ggctgacgcc atatctgtcg cggacttggc
      361 ggacattgag ggtgtcggcc gggggccggg gcgccgagat gcggtgtttg tatcgtagcc
      421 acttgatcat gctcagcgta agcggttttc cagtcgaact ttgatggccc tctgcgcgca
      481 gcagacggat gatttcgtcg tcatggtggt tgaccgccag ctgtcggatg cgcgccacga
      541 aggcctcgga ataacggacc gcctcggcac ggttgggagg catctggagc tccagcgttt
      601 cgctggcgcc gccctgccag cggacgtgca agctaataat cttaggctcg ggccccttcg
      661 tcacggtgat gtcgcgcacg agcaaacgca gtatccgctt gcgatctcgc ggcgtggtcg
      721 tcgatgcggc ccagagtctg ggcaggtcgc cggcgagttt gagaatctgc cgcttctgct
      781 cggccgtgac ggcccgcatg gtctgtcgct cgaaggcagc cagctcggct tcgagttcct
      841 gcaggcgccg catggcgtcg ttccagcgct gttcgagggt cgccgcgatg aggcgattgg
      901 cgggatcgac cgcttcgtat cggcggtcag ccaggtccac gtcgtagcgc gcccgttcga
      961 tccgcatccg ccactgcgcg ccaatctcgc gatcccgttc ctcgaggctg gtgagtgctg
     1021 cgagtgcgag gtcaatcgtc gccggcgtga tggcgccgac caacctcccg gtgatcgcct
     1081 ggtcgagtgg cgcggccgtg acgctcaggc aggcgcgcgg cgacaaggct tcgcggtgtt
     1141 tccagatgca ctgatagata ggatagacgc caccgtttcc ggtatagcgt atccccagac
     1201 ggcgcccgca ggcgccgcag agaagcatgc cctgcagcag gcagagtccc tctcgcgccg
     1261 gaccggacaa cacctcgcca ttcgtccggt tgccggcgag gcgaagccgg ttggcctcgt
     1321 accggtccca gtcgatgtag ccttcgtgat gatcacgtat catcactcgc caggcgtcct
     1381 gggggaccgg acgcgagcga gtggcgatct cgccggatgg ccccacctgc ttcgaggatt
     1441 gatagcggcc gaacacatag acgccggcgt aggaaggatt ggccaggagg ccgaggacgc
     1501 gagagtgggt caggcggccc cacagcagct tgccgttcca tgcaccacca tacgcacgac
     1561 gcgggaagcg caggccaagc tcctggaacc gtcttacaac gctgtaggct gtgccctcgc
     1621 gttcgaacaa ttcgaagacc gtgcgaaccg cgccctgcac ctcgcggtcg ggatcgagca
     1681 caattttgtc ttcgtcgaag acaaggccga ccggcagtgc aaacctcaga tcgcctctgg
     1741 atgccttgtt aagcttgccg ccgtggaggc gcgcgcggat aatgtgtaac tctgcttgag
     1801 cgaaagtgcc cttcattccg agcacgaggc cgtcgttgaa ttcggcagga cagtatacgc
     1861 catcctcgtc gattacgagc gtgccggtga tggcgcacag ttcgagcagc cgatgccagt
     1921 cctggttcga ccgcgccaag cgcgacgctt ccaaggagaa gatcgcaccg acatggccca
     1981 tcgccacgtc gccgactagg gtcttgaaat ctgtgcgggt gcttgctgca gatcccgact
     2041 tgcccagatc ccggtcgagg acccggatct gttcctggct ccagccgagt gagcgggcct
     2101 tgttcaccag attgtactgg cggtctgtgc tctcctgatt gaagcgcacc tgcgccaggg
     2161 tcgactggcg gatgtagatg caggcttgtc gcgatagatg tcgatcggca atcttgggat
     2221 gcatcgtcat tggcgctctc cggatcggat aatgaggcgc cagcagcaag aaagcagaca
     2281 accatgtcgg cgagaaccgc gcccaccttg gcgacgtcga aggaggtgag ggccgggtcc
     2341 atcacggtct acccgagatc ctcgcgccgg cgtaggagtt ccgtgttgat cgcacagatt
     2401 tcgttgagag cctcgcgcag cctgttgaag ttgccgatca aactggcaac gtgttcgtgc
     2461 gtggcgttcg ggacgtaatc tctgcggggc cgccctccgg gctgactgat gaccagatat
     2521 cgcttcggac catgtcccgg gccgctggcg cagtggcagt tcagccggcc gcagcgcttg
     2581 taggtctcgg tcagcgaacc acgcaacagc tgctcgatcg gaggcaagtc gcgtactaag
     2641 ttgcgacggc gtttgcgcag agctgttgct ggcagatccc tgatattcat cggaccgaat
     2701 cgtgatgtga tccgagagaa tcagaagtcg attcaccggt caacctccaa atcgcctgat
     2761 ctcagagcgg cgaagagctc ggcgagcgcg atcagaccga aggtatcaac cagcaaccgc
     2821 tgatcgccgg aaccggcctg ggaacccggc ggcgcccaat ctgtccagtc ccgcggcatt
     2881 gcgaacgacc ccaggtcggt gtggcgcagg ctcaacgtct ctattccgga caccgtccgg
     2941 accttcagaa cgacgaaccg ctggccgcgc aacggatgga acgggtggac gacttcagca
     3001 gaacccaaag gtcggttggg gtggagtgca gttcgcttgg cgtgtgccga cggtcacgga
     3061 tagagtcgcc caggaagtcg tcaagcggta cctggaaccc gtcctggaat tgatcttcca
     3121 tgaggattct tatggatatc ggccgggacg gtcggcgatt gacgcagtcc ggaaggcccg
     3181 tcagcgatgc tggcggtacg actgggtgct cgacgtcgat gtgcggagct atttcgatag
     3241 cattgactgg gagctgctcc taaaggcggt tcgcaaacat acggactgcc gctgggtgct
     3301 tctctacatc gaacgctggc tgaaggcccc ggtgcagctg gaggacggta gcgtcgagcc
     3361 gcgtacggcg ggaactccgc agggcggtgt tataagtccg tgcctcgcga atctgttcct
     3421 gcactacgtc ttcgacatgt ggatggtgcg aaactttccc cacatcccat tcgagagata
     3481 cgcggacgac atcatctgcc actgtcgaag cgccgaagag gcgcgggcac tgtggggcgc
     3541 cctggaggca cgcgtcgcgg cctgcaggct ggctctgcat cagcagaaga cgaagctcgt
     3601 ctactgcaag gatgcgaacc ggagaggaga tcatccgatt cagtcgtttg acttcctcgg
     3661 ctatgagttt cggccgaggt cggcgatctg gcacggtagg caactcggcg tctcgttctt
     3721 gcccgcgatc agtccgaaag cgctgaaggc tatacgg
//
LOCUS       DATAOZ010000432         5803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3332812, whole genome shotgun sequence.
ACCESSION   DATAOZ010000432 DATAOZ010000000
VERSION     DATAOZ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3332812"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="VHR64_11055"
     CDS             complement(<1..1414)
                     /locus_tag="VHR64_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3303410.1"
                     /translation="MNEKNSLSEVLDQFPKDDPLSVSRRRFIAAGAAAMATGFLATNA
                     SAAPLQGGRMTTALRQGTPPADAAPAEKQVVIFPADVGTAKVLDFYEQVYQRPFVADL
                     FSEPLVRLNRDFEIIPGVAESWEGSEDGMTWTFKLRPGLTWTDGNPLTAADWVATFQY
                     AANPEHAWDFTWFFQGVLKGWDDAIAGKAGVDTVGVAQGADPNELVITTVVPAPYIPA
                     MMLYSLPLSAAALAEHGSGLYNSDPATAVSCGPYVLSEWTPQQQIVYTKNPNYTGTMN
                     VMVEKVINRLATPDTYFTMYQNNEVDYLEEPDPASLTLMMGDETTAKEVYSGVGDFPT
                     WYIFFDVTKAPFDNKMVRQAWSHAIDRDTLKSQVLGPNGTPAYSWLAPGFPASQREAL
                     QDIQKFDPAMAKDLLSQAGFPDGKDFPKQTMWLRAPSPLDKTVAGALAAMLKENLNIE
                     VELEEHDSQGFTAALNAKPTQ"
     gene            complement(1466..2401)
                     /locus_tag="VHR64_11060"
     CDS             complement(1466..2401)
                     /locus_tag="VHR64_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303411.1"
                     /translation="MVTTNAATVAASAEPALGVRRKSRTNFQLARQRFFRQKLAVFGL
                     VLVVLFLACAIFAPLLAPTGYADANLMDANKFPSWEFPFGTDTIGHDMLSRVIYGIRT
                     SMVVAFAAVAVACLIGVPLGLAAGLKGGWLDFIVLRLVEIMTALPGILFAIFLMSVIN
                     SGAFKRIFVGGQVMNVVIVIGITSWVPLCRLMRAQILQLREQEYVTAARSIGATEYRV
                     AVSHLLPNAIPPLIIAITLAIPAAIFAEAGLSFLGIGINEPVPSLGKMVSDSRQYIQL
                     YWHLGLFPSLAIALAVLGFAFLGDGLRDALDPRQI"
     gene            complement(2404..3345)
                     /locus_tag="VHR64_11065"
     CDS             complement(2404..3345)
                     /locus_tag="VHR64_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303412.1"
                     /translation="MARYIIGRLISLIFVLLVVSFIAFVLMHAVPGGPFDTGERRLPE
                     ATRQAQLHKYGLDKSIPEQYVRYIWAVLHGDFGIPFQSPNDTVLGLIERAWPVTIRIG
                     IPTIIISFTFGTILGFVAALRQNTWVDYTVTTVATLGLTVPNFVVATWLILILSTRLG
                     WVPTGGWGEPKNYIMPVIAYSLAPTALVARYVRVSILEALHADHVRTARAKGLSEQRV
                     LRHHVARNAMIPATTVLLPEIPNILTGSIFIESVFRIPGIGRFFVTSTTSRDYPMILA
                     LVLLVAAVWGLTYLLTDILYTILDPRIRIGGASMRGG"
     gene            3649..4302
                     /locus_tag="VHR64_11070"
     CDS             3649..4302
                     /locus_tag="VHR64_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX3303413.1"
                     /translation="MNIPDVNALIPMVVESTNRGERAFDIYSRLLKDRIIFLGTPVDD
                     QIANLIIAQMLFLEHEDPNQDIRLYINSPGGVVYSGLAIYDTMQMIKPDVATFCMGMG
                     ASMAAVLLAGGTRGKRFALPNARVMIHQGSSGFRGAVPDIEVAARETLSLTNKLTEIL
                     ALHSGQEFDKVKRDTERDYYMTAADAKDYGIIDEVLEPSKLAELVASGEEQPNGKKK"
     gene            4453..5694
                     /gene="clpX"
                     /locus_tag="VHR64_11075"
     CDS             4453..5694
                     /gene="clpX"
                     /locus_tag="VHR64_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX3303414.1"
                     /translation="MHYRCSFCNKGQEEVSRLIAGPNQVYICNECVQLCREIIEEEEP
                     QTPRPAVSTPKISTPRDLYEQLNQYVVGQDRAKRILSVAVYNHYKRISAGLESDDDIE
                     LQKSNILLVGPTGCGKTLLAQTLAKLLDVPFSIADATALTEAGYVGEDVENILLKLIQ
                     NAGDVTRAQTGIIYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIIEGTIANVPPQS
                     GRKHPHQEYIQIDTRNILFICGGAFEGLEEIVGRRIGKHAPIGFGGNGEQEDKANPLQ
                     ELTHDDLLKYGLIPEFVGRLPVTVALDPLSRDELKRILVEPKNAVVKQYQKFFALDNV
                     DLAFTEEALEATARQAEERKTGARGLRTTIEEVLLDVMYEIPSMENVRKCVVDENVIV
                     NRSRPLLMTETEKQIPYAESA"
BASE COUNT         1144 a         1806 c         1851 g         1002 t
ORIGIN      
        1 tttgcgtagg cttggcgttc agcgcggcgg tgaacccctg cgagtcatgc tcctcgagtt
       61 cgacctcgat gttcaggttc tccttgagca tcgccgccag cgccccggcg accgtcttgt
      121 cgagcgggct cggcgcgcgc agccacatcg tctgcttcgg gaagtccttg ccgtccggga
      181 agccggcctg gctcaggaga tccttggcca ttgccgggtc gaacttctgg atatcctgca
      241 aggcctcacg ctgcgaagcg gggaagcctg gggccagcca ggagtaggcc ggcgtcccgt
      301 tcgggccgag cacctgcgac ttgagcgtgt cgcggtcgat cgcgtggctc caggcttgcc
      361 gcaccatctt gttgtcgaat ggcgccttgg tcacgtcgaa gaagatgtac caggtcggga
      421 agtcgccgac acctgagtag acttccttgg ccgtcgtctc gtcgcccatc atcagggtca
      481 gtgaggctgg atccggctcc tcgagatagt cgacctcgtt gttctggtac atggtgaagt
      541 acgtgtcggg tgttgcaagt cgattgatga ccttttccac catcacattc atcgtgccgg
      601 tgtaattcgg gttcttggtg tagacgattt gctgctgggg agtccactcg ctcagcacgt
      661 acggtccgca ggagactgcg gtagccgggt cgctgttgta cagaccgctg ccgtgctcgg
      721 ccagcgccgc cgctgagagc gggagcgaat agagcatcat ggccggaatg tacggtgccg
      781 gcaccacggt ggtgatgacc agctcgtttg ggtcggctcc ctgtgccacc ccgacggtgt
      841 ccacgcccgc cttgccggca attgcatcgt cccagccttt cagcacgccc tggaagaacc
      901 aggtgaagtc ccatgcgtgc tccgggtttg cggcatattg gaacgtcgcc acccagtcgg
      961 ccgccgtcag cggattgccg tcggtccagg taagccccgg gcgcagcttg aaggtccagg
     1021 tcatcccgtc ctcgctaccc tcccaggatt cggccacgcc cgggatgatc tcgaagtccc
     1081 ggttgaggcg gacgagcggc tcgctgaaga ggtcggcaac gaacgggcgc tggtagacct
     1141 gttcgtagaa gtcaagcacc ttcgccgtgc cgacatcggc cgggaagata acgacctgct
     1201 tctcggcggg cgcggcatcc gctggaggcg tgccttgacg gagcgcggtg gtcatccggc
     1261 caccctgaag cggggctgca ctggcattgg tggccaggaa tcccgtggcc attgccgcag
     1321 cgcctgcggc gatgaagcgc cgccgtgaga cggagagcgg gtcgtccttg gggaactgat
     1381 cgaggacctc cgacaaactg ttcttctcgt tcaccggtga acgcgtcctt tccttgtggc
     1441 cgacccgtcg aagggcggcc gcttgttata tctggcgcgg gtcgagtgcg tcccgcagac
     1501 catccccgag aaaagcgaat cccagcacgg ccagggcaat ggccagtgac gggaagagac
     1561 cgagatgcca gtagagctga atatactgcc ggctatccga aaccatcttg ccgagactcg
     1621 gcaccggctc gttgatgccg attccgagga agctcagccc ggcctcggcg aagatcgcgg
     1681 ccggaatcgc cagcgtgatc gcgatgatca gcggcgggat ggcattgggt agcaggtgcg
     1741 atacggccac acggtactcg gtcgccccga tgctgcgcgc ggccgtgacg tattcctgtt
     1801 cgcgcaattg caggatctgg gctcgcatca ggcggcaaag cgggacccag ctcgtgatgc
     1861 caatcacgat cacgacgttc atgacctggc cgccgacgaa gatccgcttg aacgccccgc
     1921 tattgatgac cgacatcagg aagatcgcga agagaattcc gggtagggcg gtcatgatct
     1981 cgacaagccg gagcacgatg aagtcgagcc agccaccctt gagaccggca gccagcccga
     2041 gcggtacgcc gatcaagcag gccactgcga ccgctgcgaa ggccaccacc atggaggtgc
     2101 ggataccgta gatcacgcgg ctgagcatgt cgtggccgat ggtatcagtg ccgaacggaa
     2161 actcccaact cgggaacttg ttggcgtcca tcaggttggc atcggcgtac ccggtcggtg
     2221 cgagcaacgg agcgaagatc gcgcaggcca ggaatagcac gaccagcacc aggccgaaga
     2281 cggccaattt ctggcggaag aagcgctgcc tggcaagctg gaagtttgtc cggctcttgc
     2341 ggcgcacgcc gagggccggc tcggccgaag cggcaacggt cgcggcattc gtggtcacca
     2401 tcgtcatccg cctcgcatcg atgcgccgcc gatccggatc cgcggatcga ggatcgtgta
     2461 gaggatgtcg gtaagcaagt aggtgaggcc ccagacggcc gcgaccagca gcaccagcgc
     2521 cagaatcatc gggtaatcgc ggctcgtcgt gctggtcacg aagaagcggc cgatcccggg
     2581 aatgcgaaag accgactcaa tgaagatcga gccggtcagg atgttcggga tctcgggcag
     2641 gagcacggtc gtggccggga tcatcgcgtt gcgggcgacg tggtgccgca ggactcgctg
     2701 ctcagagagc cccttcgccc gcgccgtgcg gacgtgatca gcatggagcg cctccaggat
     2761 cgagacgcgc acgtacctcg ctaccagcgc ggtgggcgca agcgaatagg cgatgaccgg
     2821 catgatgtaa ttcttgggtt cgccccatcc tcctgtcggc acccaaccca atcgcgtgct
     2881 gagaatcagg atcagccagg tcgcgacgac gaagttgggg acggtcaggc cgagcgtggc
     2941 cactgtcgtt accgtgtaat cgacccacgt gttctggcgc agagcagcga cgaagccgag
     3001 gatcgtgccg aaggtgaacg agatgatgat cgtgggtatg ccgatccgga tcgtgaccgg
     3061 ccaggctcgc tcgatcaggc cgagcaccgt gtcattcggg ctctggaacg ggatgccgaa
     3121 atcgccgtgg agcaccgccc agatgtatcg cacgtattgc tcggggatcg acttgtcgag
     3181 accgtacttg tgcagctggg cctggcgggt cgcctccgga aggcggcgct cgccggtatc
     3241 gaagggaccg ccggggactg cgtgcatcag cacgaacgca atgaacgaga cgacgagcag
     3301 cacgaatatc agactgatca ggcgaccaat gatgtatcgc gccacggaat cgacccctga
     3361 ggttccagat ttgaagtagc gctgagtcta gcacgctgcc tgcgccaggg ccgggtggtg
     3421 cgcaggcgta actacctacc gggaatcggg atgaacgtac accccgtttg tcctatcctt
     3481 gctcgacacg acggagtatg attgagtcct gaggcgctca ttggctgagc gacaatcacg
     3541 agtctcgcgg aatatcccgc atgctgtccc attcacgggg cggaaagcag accaactggt
     3601 ggcgtacggg ttgctggcaa tcgccgccac atgaggacac acatcgacat gaacatccca
     3661 gacgtgaacg ccttgatccc gatggtggtg gaaagcacca accgcggcga gcgcgcgttc
     3721 gatatctact ctcgcttgct gaaggaccgc atcatcttcc tgggcactcc ggttgacgac
     3781 cagatcgcga acctgatcat cgcccagatg ctcttccttg agcacgagga cccgaaccag
     3841 gacattcgcc tctacatcaa ctcaccgggc ggcgtggtct actccgggct cgccatctac
     3901 gacaccatgc agatgatcaa gccggacgtg gccaccttct gcatgggcat gggcgcgagc
     3961 atggccgcgg tcctactggc cggcggcacg aggggcaagc ggttcgcatt gccgaacgcc
     4021 cgcgtcatga tccaccaggg ttcctccggt ttccgaggcg ccgtgccgga tatcgaagtg
     4081 gccgcgcgcg agacgctgag cctgaccaac aagctcaccg aaatcctggc cctgcactcc
     4141 ggccaggagt tcgacaaggt caagcgcgac accgagcgcg actactacat gaccgcggcc
     4201 gacgccaagg actacggcat catcgatgag gtgctggagc catccaagct cgcggagttg
     4261 gttgcctcgg gcgaggagca gccgaacggg aagaagaagt agcgggaagg atttccgctc
     4321 ccggtgggga cgttgcggct atcaggcacg tccccatcgc caaccccggt cgcgtttaat
     4381 tcgcaccggc agttctgata gcaagtgcta acgcggccgg tccacggcgc ttatctacac
     4441 cgaggtgagc tggtgcacta tcgatgttcg ttctgcaaca agggccagga agaagttagc
     4501 cggctgattg cagggccgaa tcaggtctac atctgtaacg agtgcgttca gctctgtcgc
     4561 gagatcatcg aggaagagga gccgcagacc ccgcggccgg ccgtgtcaac gccgaagatc
     4621 tcgacccccc gagatctcta cgagcagctc aaccagtacg tcgtcgggca ggaccgcgcc
     4681 aagcggatcc tctcggtcgc cgtctacaac cactacaagc gaatcagcgc cggcctcgag
     4741 agcgacgacg atatcgagct ccagaagagc aacatcctgc tggtcgggcc gaccggctgc
     4801 ggcaagacac tgctcgccca gacccttgcc aagctcctcg acgtgccctt ctcgatcgcc
     4861 gatgccaccg ccctcaccga ggccgggtat gtcggcgagg atgtcgagaa tatcctgctc
     4921 aagctgatcc agaatgccgg cgatgtgacc cgggcccaga ccggcatcat ctacatcgac
     4981 gagatcgaca agatctcgcg caagtccgat aacccgtcga tcacgcggga cgtctccggc
     5041 gagggcgtcc agcaggcgct cctcaagatc atcgagggga cgatcgccaa cgtgccgccc
     5101 cagagcggcc gcaagcaccc gcaccaggaa tacatccaga tcgatacgcg caacatcctc
     5161 ttcatctgcg gcggcgcctt cgagggactt gaggagattg tcggccgccg gatcggcaag
     5221 cacgcgccga tcggcttcgg cggcaacggc gagcaggagg ataaggccaa cccgctccag
     5281 gagctaaccc acgacgactt gctcaagtac gggttgatcc ccgagtttgt cggccgcctg
     5341 cccgtgacgg tggcgctcga tccgctcagc cgggacgagc tcaagcgtat cctggtcgag
     5401 ccgaagaatg cggtcgtcaa gcagtaccag aagttcttcg cgctcgataa cgtcgacctc
     5461 gccttcaccg aggaggcgct tgaggcgacc gcccgccagg ccgaggagcg caagaccggc
     5521 gcccgcggcc tgcgcacgac catcgaggaa gtgctgctcg acgtcatgta cgagatcccg
     5581 tcgatggaga acgtgcgcaa gtgcgtcgtc gacgagaacg tgatcgtcaa ccgcagccgc
     5641 ccgcttctga tgaccgagac cgaaaagcaa atcccctacg ccgaatcggc ctaggatcgg
     5701 tattcggtac ttgctgttcg ggaacgaacc gccgccacct cgaccgggct gatcgacacg
     5761 gtctccgggc cgaacggtgt gacgagcaat tacgactacg acc
//
LOCUS       DATAOZ010000433         4423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1757972, whole genome shotgun sequence.
ACCESSION   DATAOZ010000433 DATAOZ010000000
VERSION     DATAOZ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757972"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VHR64_11080"
     CDS             <1..463
                     /locus_tag="VHR64_11080"
                     /inference="COORDINATES: protein motif:HMM:NF026044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303415.1"
                     /translation="AKQPVFIREVQLEPVRHDPLHIDFFAPNLRKELTASVPVVLHHQ
                     NPNAEGIFNHVHTEIQLRGLPAAMPHQIDADISELVAVGDTLRVADLTLPEGVVSVTG
                     EDEVIVSIVPQRVEEVEEVEEEAVEGEEVAAKEAATEEPAAEAESTESEAE"
     gene            complement(724..1179)
                     /locus_tag="VHR64_11085"
     CDS             complement(724..1179)
                     /locus_tag="VHR64_11085"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX3303416.1"
                     /translation="MARKEEPELVYQFRVAAQDDGTYAIWFPDLPGCSTSATSVDEIG
                     PVALEVMQLWIESEVEQGRPLPDMSKRPDVLWPHEAYLVDEEEAPSYLLARDIASQLG
                     VTERRVSAIAQRRGIGTMVGRLRLFRPDDIEALRPGPVGRPSHRANAIA"
     gene            complement(1228..1467)
                     /locus_tag="VHR64_11090"
     CDS             complement(1228..1467)
                     /locus_tag="VHR64_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303417.1"
                     /translation="MNRQKVIERLLSPNPPKELRVDVVLGIYLNDLGWTYRKKVGSHH
                     QLTKPGYRTEVVTEHGNKVGEEVIRKLIVHLKAGQ"
     gene            complement(1552..2394)
                     /locus_tag="VHR64_11095"
     CDS             complement(1552..2394)
                     /locus_tag="VHR64_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303418.1"
                     /translation="MIGSISAELFLLRKRAATWILLGIWTALAAFFAYILPYVSYRNE
                     TNTPDRFREDLVDLLPQSIVANVLGGFPFFGGVLVLILGALTLGSEYGWGTLKTLFTQ
                     RPVRLNVFAAKLVALGIWLIPFVVLSFAVGAIASYLIARAEDASVDWPSAWLIVRGLA
                     AAWLIMAVWAALGVLLGVLSRGTALAIGIGIIYGLVIEGLISALLDQVDVLSSLIKAL
                     LRANAYSLIAVLGTSTSDISGNGPGSFSGPFVGGVQSVLVLALYLAAFVAIAATVLQR
                     RDVS"
     gene            complement(2391..3395)
                     /locus_tag="VHR64_11100"
     CDS             complement(2391..3395)
                     /locus_tag="VHR64_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3303419.1"
                     /translation="MVHTSIDLGTSTEMTPALHDRRASPNGVDDDLVVETRGLTKRYG
                     ERIVAVSDLALRVRRGEVYGFLGPNGAGKTTTLRMLLGLIRPSAGTALVLGEPPGSSR
                     GLARLGAMIEAPAFYPFLSARDNLRVLAKYAGVPESRIGPVLDEVDLASRAGDKFGTY
                     SQGMKQRLGVAAALLKDPELLILDEPTNGLDPAGMAEMRTLIRDLGRGRHTVLLSSHL
                     MTEVEQVCDRVGVIRGGQLVAEGTVDELRARTREGLIVRAEPFAEAKRIVASYEPLDK
                     IEERDGALRIDTDPRRAAEINRALVSAGIAVSELRPERASLEEVFFELTRETDTGETS
                     "
     gene            3462..4079
                     /locus_tag="VHR64_11105"
     CDS             3462..4079
                     /locus_tag="VHR64_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3303420.1"
                     /translation="MSPRPYRLDRRQAAAEETRARIITAARELLSAGDGVAGFSIDAV
                     AREAGVARMTVYYQFSSKRGLYEALADNLATRAEIGERLRVAFRQEDALDALDAFIAA
                     FGHFWTPDRVLIRRLHGVAATDPEIGRGELERNERRRSGLRVVLERVAAQYGRPESFD
                     EAVDVLHTLTSFETFDSLAGTTRSPEEVVAVVQRLARAVLGLPNR"
BASE COUNT          847 a         1361 c         1379 g          836 t
ORIGIN      
        1 cgccaagcag ccggtcttca tccgcgaagt tcagctcgag ccggtccgtc acgatccgtt
       61 gcacatcgac ttctttgcgc cgaacctgcg caaagagctc accgcgagcg tgccggtcgt
      121 gctgcatcac cagaatccga atgccgaggg cattttcaat cacgtccaca ccgagattca
      181 gcttcgtggt ctgccggcgg cgatgccgca tcagatcgat gccgacatct cggagcttgt
      241 ggcggttggt gataccttgc gtgttgccga tctcaccctg ccggaaggcg tggtctcggt
      301 gaccggtgag gacgaggtga tcgtcagcat cgtgccgcag cgcgtcgaag aggtggaaga
      361 ggtcgaagaa gaggctgtcg agggcgagga ggttgctgcc aaggaagccg ccaccgaaga
      421 gccagcggcc gaggccgaat caaccgagag tgaagccgaa tagcgacaca tatccctaaa
      481 gcacacaaag acgcccggcc atttggccgg gcgtcgtttg ttgctatcgc aatcacccga
      541 gatgagatcc tgcacagtct tgcgctcccg ttggggcacg cgctagttgt ccacgctcgt
      601 tcggcgtccg atcctggatt ttctgctggt tccgcggtcg ctggccgcac taaatatcga
      661 gatgtttggg tgccctctgg gcagatgaaa tacccctgac cattgtgatc aggggtgttg
      721 gtcttaggcg atggcgttcg cccggtgcga tggtcgtccg accgggccgg gccgcagcgc
      781 ctcgatatcg tcgggacgga acagccggag ccgtccgacc atggtgccga tgccccggcg
      841 ctgcgcgatc gcgctcaccc ggcgttccgt gacgccgagt tgtgatgcga tatcgcgcgc
      901 caggagatag gagggcgctt cctcctcatc gaccaaatag gcctcatggg gccacaacac
      961 atccggtcgt ttactcatat caggcaacgg gcgcccttgt tcaacctccg attcaatcca
     1021 gagttgcata acttctaagg ccactggtcc aatttcgtct accgatgttg ccgaggtgct
     1081 gcatcccggc agatcgggga accagatggc atacgtgcca tcgtcttgtg cggccacacg
     1141 gaactggtag accagctccg gttcttcttt tctggccatg gttccagttg cgtcctttcc
     1201 ttcctcctgt ggcctgagtg gccgggatta ctgcccggcc ttcaggtgga cgatgagctt
     1261 ccgtatcacc tcttccccta ccttgttgcc atgttcggtc accacttcgg tgcgatatcc
     1321 tggcttcgtc agttggtggt ggctccccac cttcttccgg taggtccatc ccaggtcgtt
     1381 cagatagatg ccaagtacaa catcgacgcg aagttccttg gggggattag ggcttaacag
     1441 ccgctcgata actttttggc gattcaatgc ctcgccttca cacggtcgac accagcccgt
     1501 cgctggatca gtaactatac tctcatcgga atagtttatc aaggatcgag atcaggagac
     1561 gtcgcgtcgc tggagcaccg ttgcggcaat cgcgacgaat gctgctagat acagggcgag
     1621 cacgaggacg gattgcacgc ccccgacaaa gggaccggag aacgaacctg gtccgttgcc
     1681 cgagatgtca ctcgtcgatg tgccgaggac ggcaatcagt gaatacgcgt tggcgcgcag
     1741 cagcgccttg atcagcgagc tcagcacgtc gacctggtcg agcagtgcgc tgatcaagcc
     1801 ttcgatgacc agcccgtaaa taatgccaat cccaattgcg agggcggtac cacgcgaaag
     1861 aacgccgagc aacacgccga gtgcagccca cacggccatg atcaaccagg cggcggccaa
     1921 cccgcgcaca atcagccagg cgctcggcca gtcaactgac gcatcctcgg cgcgagcgat
     1981 gagataactg gcgattgccc cgacggcgaa gctgaggacg acaaacggaa tgagccagat
     2041 accgagcgcc acgagcttgg cggcgaaaac gttcaaccgg accgggcgct gggtgaagag
     2101 cgtcttcagc gttccccagc catactcact gccgagggtc agcgcgccga gaatcaacac
     2161 cagcacccca ccgaagaagg ggaacccacc aaggacgttg gcgacgatgc tctgcggcag
     2221 caagtcgact aggtcctcgc ggaaacggtc cggcgtgttc gtttcattgc ggtagctgac
     2281 gtagggaagg atataggcga agaacgccgc cagcgccgtc cagattccga gcaggatcca
     2341 ggtcgcggcg cgcttgcgca gcaggaacag ctcggcggaa atacttccga tcatgatgtc
     2401 tctccagtat cagtttcccg ggtcagttcg aagaacactt cttccagtga ggcgcgctct
     2461 gggcgcaatt cgctcaccgc gatcccggcg ctaaccagag cccggttgat ctctgcggcc
     2521 cgccgcggat cggtatcaat ccggagtgcc ccgtcgcgtt cttcgatctt gtctaggggc
     2581 tcgtagctcg ccacgatgcg tttcgcctcg gcgaatggct cggcccgcac gatcagtcct
     2641 tcgcgcgttc tggcccgcaa ttcgtcgacc gttccctcgg ccacgagttg gccgccgcgg
     2701 atgacgccga cgcgatcgca gacctgttcg acctcggtca tcaggtgact cgacaaaaga
     2761 accgtgtgcc ggccgcgacc gagatcgcga atgagtgtcc gcatctccgc catgccggcc
     2821 ggatcgagcc cattggtcgg ctcgtcgagg atgagcagct cagggtcctt gagcagcgcg
     2881 gcggcgacgc cgagccgctg cttcatgccc tgcgagtagg ttccaaattt gtcgccggcg
     2941 cgcgatgcca gatcgacctc atcgaggacg ggtccgatcc gcgactcggg aacgccagcg
     3001 tatttggcca agacacgcag attgtctcga gccgagagga agggatagaa ggcgggcgct
     3061 tcgatcatcg cgccgaggcg ggcgagaccg cgtgaggagc cgggtggctc gcccagaacc
     3121 agcgccgtac cggcggaggg tcgaatcagg ccgagaagca tccgcaaggt tgtcgtcttg
     3181 ccggcgccat tcggaccgag aaacccgtag acctcgccgc ggcggacgcg gagcgcgaga
     3241 tccgagacgg cgacgatccg ctcgccatag cgcttggtga gaccacgggt ttcgacaacc
     3301 aggtcatcgt caacgccgtt cgggctggcg cgccggtcgt gtagggcggg ggtcatctct
     3361 gtcgaagttc cgagatcgat tgatgtgtgc accatgaatg cctccttgca tttagacgta
     3421 ctgtaagtat aatcaaattg caaagaggag atcaaacagc aatgagtccg cgcccttacc
     3481 gtcttgaccg tcgtcaggcg gccgctgaag aaacgcgcgc gcgcatcatc acggccgccc
     3541 gtgaactcct ttcggccggc gatggcgtgg ccggctttag catcgatgcg gtcgcgcgcg
     3601 aggccggcgt cgcccggatg acggtctact atcaattcag ctcgaaacgc gggctctatg
     3661 aggccctcgc cgacaatctc gccacccggg ccgaaatcgg cgagcgcctc agggtcgcgt
     3721 tccgccaaga ggatgcctta gacgcgttgg atgcgtttat cgccgccttc ggtcactttt
     3781 ggactcccga ccgggtcctc atccgccgcc tccacggggt ggccgcgacc gatcccgaaa
     3841 ttgggcgcgg agagctcgag cggaacgaac gccggcgcag tggattgcgg gtcgtgctcg
     3901 aacgggtggc tgcgcagtac ggtcgaccag agagcttcga cgaagcggtc gacgtcctcc
     3961 ataccttgac gagtttcgag accttcgaca gtctggccgg cacgacgcgc tcgcccgagg
     4021 aggtcgtcgc ggtggtgcag cggctcgccc gcgcggtgct tggattgccg aatcgttagc
     4081 gcgggtatta caaggagtgt cgtgtcgggc atgccccgag tttctaaccc ggggctaaca
     4141 cgacgaagaa ttccctaggt tgaaacctag ggctaagaca acgaagcctc tccgaggctg
     4201 agtcaaccct cacccgccgc tggcgcggct gccctcttcc tgatctgcag ggagaaggca
     4261 cgcactgggc gcgtactcgt ccaaacggct cccctcgccc tgcggatcag ggagaggggt
     4321 tgggggtgag ggttgcatag tcccaccggg acttcgtcgt gttagcgccg gacttctagt
     4381 ccggcgaagt ggtcttaagc cggcgtggcg gcaaccggtt cct
//
LOCUS       DATAOZ010000434         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2806701, whole genome shotgun sequence.
ACCESSION   DATAOZ010000434 DATAOZ010000000
VERSION     DATAOZ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806701"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VHR64_11110"
     CDS             <1..275
                     /locus_tag="VHR64_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303421.1"
                     /translation="HSSMIGKIEKAHRYAREPERVRLNSLNATFHGSHDEYTIELNDG
                     HWTCSCHTFSSHVVGTCSHVMALQQMLGQMLAPDLRYDVEEHAAAV"
     gene            complement(280..645)
                     /locus_tag="VHR64_11115"
     CDS             complement(280..645)
                     /locus_tag="VHR64_11115"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX3303422.1"
                     /translation="MKLVIAIIQDYDTDAFLKAISRQGLMATRIASAGGFLRTGNTTV
                     FLGVEDDQVPLVKRLLVDHCRVREEQAVAEALPFLDDADFEHISISRIGGGVAFVARV
                     ERFERFARATNSPSETTVT"
     gene            complement(724..1824)
                     /gene="leuB"
                     /locus_tag="VHR64_11120"
     CDS             complement(724..1824)
                     /gene="leuB"
                     /locus_tag="VHR64_11120"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX3303423.1"
                     /translation="MSQPTYDLVLLPGDGVGPEIIAAAQTVMQAVESRFAVTVKTDTR
                     LIGGAAIDAHGVPLRAEDLDRCNQADAVLLGAVGGPKWDDPRAKVRPEQGLLQLRKGM
                     GLFANLRPVAVMPSLADRAPIKQELLRGVDMMVVRELTGGVYFGKPRRSWRERRGRAA
                     LDTLIYREYEVERICRLAFEIARGRKGKLASVDKANVLDTSRLWRSVASEMAPAFPDV
                     ELEHVLVDSMTMHLIREPARFDVVVTENMFGDILTDEASVLAGSIGLLPSASIGAAQS
                     NGEGRRRGVYEPIHGSAPTIAGKGLANPVGTILSLAMLFRLSVLRDDIAAAIEAAVAQ
                     VIDSGRRTPDIADAGSEILTTSGVGEAVAAAI"
     gene            complement(1847..2629)
                     /locus_tag="VHR64_11125"
     CDS             complement(1847..2629)
                     /locus_tag="VHR64_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX3303424.1"
                     /translation="MTRVSVVELPDNRDRFELAWPALLQHVDFAESDVVVLPELPAGA
                     WFGTKPAFDPKRWDRVVAAHDELVSRLGEFGNAVVIGSRAANVNSQRRNIAFVWSKET
                     GLIDLHPKAILPEEPGFHEQSWYHAGDDAPTVVDVRGMTIGVLLCSELMHTERARKLG
                     DAGATMIGVPRATGDHQRWQIASQMAAIASGAFVLSSNRSGQSQDDASVIFGGRSMIV
                     DPDGTILTETGRDKQFATASIDLDASMAAKSTYPRYLDFGDR"
     gene            complement(2626..3234)
                     /gene="leuD"
                     /locus_tag="VHR64_11130"
     CDS             complement(2626..3234)
                     /gene="leuD"
                     /locus_tag="VHR64_11130"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX3303425.1"
                     /translation="MQAVRKITGVVAPLDRSDVDTDQIIPASYLKRVERTGFGEGLFA
                     SWRQNDPDFVLNKPEFKGASILVAGPNFGTGSSREHAVWSLMDYGFQAVVSPRFGDIF
                     RNNSTKSGLIPVQVDQEVADLLLKAAAEEPGLQMTIDVESRTLSVPEIGVEVEFPLDE
                     FTQYRFLNGLDDIGLTLQHQDDIAAFEARRPDYKPVTIGGAQ"
     gene            complement(3239..>3961)
                     /locus_tag="VHR64_11135"
     CDS             complement(3239..>3961)
                     /locus_tag="VHR64_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX3303426.1"
                     /translation="GAKAGMIAPDETTFAYVEGRPHAPKGVAWEQALDAWRTLPTDDG
                     ANFDHEVTLDGTKIAPHVSWGTNPGQVISINGSVPSPDQFDDPKSREAAARALAYMDL
                     KAGTPMREIAVDTIFIGSCTNSRIEDLRLAAGVVGGRKVRNGIRAMVVPGSMRVKAEA
                     EQEGLDRIFLDAGFEWRAAGCSMCLGMNPDQLSPGERCASTSNRNFEGRQGKGGRTHL
                     VSPAVAAATAIVGTFATPADLG"
BASE COUNT          744 a         1357 c         1153 g          707 t
ORIGIN      
        1 tgcactcgag catgatcggc aagatcgaaa aggcacatcg ctatgcccgc gaacccgagc
       61 gcgtacgcct caatagtctg aatgccacct tccacggcag tcacgacgaa tacacgattg
      121 aacttaatga cggacactgg acgtgtagct gtcacacctt ctcgtcacac gtcgtcggca
      181 cctgcagcca cgtaatggcg ctccagcaaa tgctcggaca gatgctggcc cccgaccttc
      241 gctacgacgt tgaggagcac gcggcggcgg tctgaccgct caggtgactg tcgtttccga
      301 aggactgttc gttgcccggg caaacctctc gaatcgctcc acccgggcaa cgaacgccac
      361 accgccgccg atgcgagaga tcgagatatg ctcgaaatcg gcatcgtcca ggaacggtaa
      421 cgcttcggcc actgcctgct cttcccgaac ccggcaatgg tcaaccagga gccgcttcac
      481 cagcggtacc tgatcatcct cgacccccag aaagacggtg gtgttgccag tgcgcaggaa
      541 accaccagcg ctcgcaatcc gcgtcgccat cagaccctga cggctgatcg ccttcaggaa
      601 cgcatcggtg tcgtagtcct ggatgatcgc gatgacgagc ttcatcggcc gttaccttcg
      661 atcaagcccc accgattcca cgccatcgtg aatatcccga cccgcattgt cgcggccttt
      721 ctctcagatc gctgctgcga cagcctcacc aacgcctgag gtcgtcagaa tctccgaacc
      781 cgcatcggca atatccggcg ttcgccgtcc agaatcgatg acctgcgcta ccgccgcctc
      841 aattgccgcc gcgatatcgt cccgcagcac cgagagccgg aacagcatcg cgagtgaaag
      901 gatggttccg accggattgg ccaaaccctt cccggcaatc gtcggcgcgc tgccgtggat
      961 cggctcgtag acaccccggc gacgtccctc tccattcgac tgtgcagctc cgatgctcgc
     1021 cgagggcagc aaaccaatcg acccagctaa tacggatgcc tcgtccgtca ggatgtcgcc
     1081 gaacatgttc tcagtgacga ccacgtcgaa gcgtgccggt tcgcggatca aatgcatggt
     1141 catcgagtcg accagcacgt gctcgagctc gacgtcgggg aaggccggcg ccatctcgct
     1201 cgctaccgat cgccagaggc gagacgtgtc gagcacattc gccttgtcga cggacgcgag
     1261 tttgcccttg cggccccgcg caatctcgaa cgccagccgg caaatccgct ccacctcgta
     1321 ctcgcggtag atcagcgtgt cgagcgcggc tcgaccccgg cgctcccgcc acgaccttcg
     1381 cggcttaccg aaatagaccc cgccggtcag ctcccggacg accatcatgt cgacaccgcg
     1441 cagtaactct tgcttgatcg gggcgcgatc ggccagagac ggcatcacgg cgaccggccg
     1501 caggttcgcg aagaggccca tccccttgcg cagctgcaag agcccctgct ccggtcgcac
     1561 cttcgccctg ggatcgtccc acttcggccc gccgaccgct ccgagcagaa ccgcgtcggc
     1621 ctgattgcag cgatcgaggt cctcagcacg gagtggcacg ccatgcgcgt caattgccgc
     1681 gccgccgatc aaccgcgtgt cggtcttgac cgtgaccgcg aaccgggatt caacggcttg
     1741 catgacggtc tgggcggcag cgatgatctc cgggccgacg ccgtcgccgg gaagcaagac
     1801 aaggtcgtat gtgggctgag acaagctcgt gtactccaga cgttcgttag cgatcgccga
     1861 aatcgagata gcggggatac gtggatttgg ccgccatgct ggcatcgaga tcgatcgacg
     1921 ccgtggcgaa ctgcttgtcg cggccggtct cggtcaggat cgtaccgtcc ggatcgacga
     1981 tcattgagcg gccgccaaag attaccgacg catcgtcctg ggactgaccg gaccggttcg
     2041 acgacaggac gaacgcgcct gaggcaattg cagccatttg gctggcgatc tgccagcgct
     2101 ggtgatcgcc ggtagcgcgt ggcacgccga tcatggttgc gccggcatcg ccgagcttcc
     2161 gcgcccgctc ggtatgcatc agctccgaac agagtagaac accgatcgtc atcccacgga
     2221 catcaacgac cgttggggca tcgtcgcctg cgtggtacca cgactgctca tggaaacccg
     2281 gctcctcggg caggatcgcc ttcggatgca ggtcgatcag ccccgtctcc ttcgaccaga
     2341 cgaaggcgat gttccgacgc tgcgaattca cgttggccgc gcgggatccg atcacgaccg
     2401 cgttgccgaa ttccccgagc cgcgacacca gctcgtcatg cgcggcgaca acccgatccc
     2461 agcgtttcgg atcaaacgcc ggcttcgtgc cgaaccatgc gccggctggc aattcgggca
     2521 gcacgacgac atcactctcg gcgaaatcga catgttgcag gagcgccggc caggccagct
     2581 caaaacgatc gcgattatcc gggagctcga cgaccgagac gcgcgtcact gcgcgccgcc
     2641 gatcgtcacc ggcttgtagt ccggtcggcg cgcttcgaag gcagcgatgt cgtcctggtg
     2701 ctggagcgtc agaccgatat catccagacc gttcaggaag cggtattggg tgaattcgtc
     2761 cagcgggaac tcgacctcga ctccgatctc cggcaccgag agcgtccggc tctcgacgtc
     2821 gatcgtcatc tgcaaacccg gctcctcggc cgcagccttc agcagcagat cggcaacctc
     2881 ctgatcgacc tggaccggaa tcaacccgct ttttgtcgag ttgttccgga agatgtcgcc
     2941 gaagcgtggt gagacgacgg cctgaaaccc gtaatccatc aacgaccaga ccgcatgctc
     3001 ccgggatgag ccagtgccga agttcggccc ggccacgagg atgctcgcgc ccttgaactc
     3061 gggcttgttg agcacgaaat caggatcgtt ctgccgccac gaagcgaaca gtccctcgcc
     3121 gaacccggtc cgctcgactc gcttgaggta ggaggcgggg atgatctgat ccgtgtccac
     3181 gtcactgcgg tcgagcggcg ccacgacgcc cgtgatcttg cgtactgcct gcattcccct
     3241 accccaaatc cgccggcgtc gcgaacgtgc ccacaatggc agttgctgcg gccactgccg
     3301 gcgataccag gtgcgtgcga ccgcccttgc cctgccggcc ttcgaagttc cgattactgg
     3361 tcgaggcgca gcgctcgccg ggcgagagct gatcggggtt catacccagg cacatcgagc
     3421 acccggcggc ccgccattcg aagccggcat caaggaagat ccgatccagc ccctcctgct
     3481 ccgcctccgc cttgacccgc atcgagcccg gtacgaccat cgcccggatc ccgtttcgca
     3541 ccttccgacc tccgaccacg ccggctgcca gccgcaaatc ctcgattcgg ctgtttgtgc
     3601 aagatccgat gaagatggtg tcgaccgcaa tctctcgcat cggcgtgccg gccttcagat
     3661 ccatatatgc gagtgctcgc gcggcagctt cgcgcgactt cgggtcatcg aactggtccg
     3721 ggcttggcac cgagccgttg atcgagataa cctggcccgg attcgtgccc cacgagacat
     3781 gcggcgcaat cttcgtgccg tcaagcgtca cctcatggtc gaagttcgcg ccgtcgtcgg
     3841 tcggcaatgt acgccaggcg tccagggcct gctcccaggc gacgcccttc ggggcgtgtg
     3901 gtcgaccttc gacataagcg aacgttgttt catccggcgc gatcatgccg gccttggctc
     3961 c
//
LOCUS       DATAOZ010000435         9494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2282817, whole genome shotgun sequence.
ACCESSION   DATAOZ010000435 DATAOZ010000000
VERSION     DATAOZ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2282817"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..888)
                     /gene="plsY"
                     /locus_tag="VHR64_11140"
     CDS             complement(268..888)
                     /gene="plsY"
                     /locus_tag="VHR64_11140"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX3303427.1"
                     /translation="METPLGIAVLVLAYLAGAIPVGVILGKLFYGTDLRTIGSGSTGT
                     TNALRAYGPKISALVLFFDILKGAIPVLIAHALDRGPWLVGAVAVLTVVGHCWSPFIE
                     FDGGKGVATGAGALIALQPWVAFAVFLMVAIVLLTHYVSLGSIVTAVSIGIALSIAAL
                     SDRVADPIAIAAVIIAAIIVWRHRGNIDRLIHGSERRISFRNRAAA"
     gene            complement(890..2227)
                     /gene="der"
                     /locus_tag="VHR64_11145"
     CDS             complement(890..2227)
                     /gene="der"
                     /locus_tag="VHR64_11145"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX3303428.1"
                     /translation="MARPIVAIVGRPNVGKSTLFNRLVGERRAIVEDIPGTTRDRLYG
                     TTTWGGVEFTIVDTAGLQDPSEIEASSTAEIAQMTRDQARSAIDEADVIVFMVDTKAG
                     MTAGDHEVADMLRRTDKPTILVANKVDNINRKDMAVEFYELGLGDPMAMSSYHGVGTS
                     DLLDRIVENLPHFEEEPEIAGPKIAIVGRPNVGKSRLVNALIGEERSIVSDVPGTTRD
                     SLDTHMEWNGRPLTLIDTAGIRRRGRIEQGIEHFSVLRSMRAIDRSDVVLLVVDSTEG
                     FTAQDLHIAGYVEEQKKGIVVIVNKWDLIEKDSSTMDLYRDEAKRQLDFLPYAPLVFI
                     SAKFGQRVQNVLEMALLVEMERRKRVSTSALNKLLREAVAAHAPASKPGKWLKFYYAT
                     QADVSPPTFVFFCNDPQSVHFSYKRYLENQIRDQFGFVGNPVRMAFRGREEDE"
     gene            complement(2382..3728)
                     /gene="gcvPA"
                     /locus_tag="VHR64_11150"
     CDS             complement(2382..3728)
                     /gene="gcvPA"
                     /locus_tag="VHR64_11150"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX3303429.1"
                     /translation="MTFNPHTEQDRAEMLAAVGVASVEELFSGIPEDIRRPDLHLPPA
                     LTEMEAAARLTELAGKNLQIAPGNSFLGAGSYSHYVPALVNQLLLRGEFFTAYTPYQP
                     EVAQGTLQVIYEFQSMVAALLGTEVANASLYDGATAVAEAVLMAIASVKKRERIVIAS
                     TVHPHYREVLQTYVSGRHLEITEVPLPDASSLLTSVDAVKAELGDDVACVVIQYPNFY
                     GGIEDVRAVAEAVHEAGALLIVSTLPVPLGVLTPPGEMGADFVTAEGQSLGVAQSFGG
                     PYVGLLGTRKSFVRQMPGRLAGVATDSEGKRGFVLTLQTREQHIRREKATSNICTNQG
                     LMATAATIYMSALGPQGLGEVAERCYQNAHYLADQVTSLPGYERAGDGEFFHEFVVKT
                     PMAPAELNRTLAEHGIIGGLDLGRYEEALAGHWLLCATELNDRAAIDRLIGALREI"
     gene            complement(3756..4145)
                     /gene="gcvH"
                     /locus_tag="VHR64_11155"
     CDS             complement(3756..4145)
                     /gene="gcvH"
                     /locus_tag="VHR64_11155"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX3303430.1"
                     /translation="MAVPGDLKYTKSHEWARVDGDIVTMGMTDYAQGELGDITYLELP
                     EPGESISADEPFGVVESVKAASDIYAPVTGEIVERNEGAVSGPEVVNASPYEQAWLVK
                     IRVGDQSGLDNLMSPDEYEKYLEEVAH"
     gene            complement(4168..5289)
                     /gene="gcvT"
                     /locus_tag="VHR64_11160"
     CDS             complement(4168..5289)
                     /gene="gcvT"
                     /locus_tag="VHR64_11160"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX3303431.1"
                     /translation="MADDTLRRTPLFDRHVALGARIIPFAGWEMPVQYSGIIAEHNAV
                     RNQAGLFDLGHMGQVDVTGPDALAYLQATTTNDVSALQPGEAQYAMLPNEHGGVIDDI
                     LIYRRPEGGDYMVVVNASNRFRDVAWMQEQRERLEDLNVTVRDISDETAMIAIQGPNA
                     AAITQSISGDDLSDIAHYHWRSGTVAGIPVMIARTGYTGEDGFEIYGPIDTAGTIWDV
                     LMEAGKEHGLQPIGLGARDTLRLESRMPLYGQELGDDISPYEAGLGWAVKLNKGDFVG
                     REGMAQVKSEGPKRKAVGFRMTERSGAPRTHYPVGIDGNVIGEVTSGAFSPTLGENIG
                     LALIAPEYAGVGKPLQVEIRGKWINAEQIKMPFYKRSGQ"
     gene            complement(5303..6715)
                     /gene="purF"
                     /locus_tag="VHR64_11165"
     CDS             complement(5303..6715)
                     /gene="purF"
                     /locus_tag="VHR64_11165"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX3303432.1"
                     /translation="MPEDIWDDKPTEECGVFGIFAPEQDVARITFFGLYALQHRGQES
                     AGIATSDGEHVSIRTRIGLVSQAFSEDDLALLPGHIAVGHTRYSTQGSNSPRNAGPMV
                     SGSNLGPIVVAHNGNVTNALNLREELEHEGERFLTTTDSEILTRLIALSPGFSFVDKI
                     RNAMTRMLGAYSLAILTRDRLFAVRDPLGVRPLCLGKIDDNWVVASESCALATVGATF
                     VREVEPGEIVEIKGATANSHTQEFPRKQAMCLFELIYFARPDSLIHGQRLHQVRQRMG
                     AQLSREFPIEADVVVGLPDSATPAAIGYAQESGIPYAEALIKNRYIGRTFIQPDQRLR
                     DQGVSLKFNALPEAIAGKRVVLVDDTIVRGTTSRPIVQLLRNAGAKEVHMRVHAPPMM
                     WPCYLGVDLARRDELIAARMTVPEIGRFIGADSIGYLSLEGLLEAIKAPDAGFCTGCL
                     TGNYPVNVQMGLDKLVLERV"
     gene            complement(6728..8941)
                     /gene="purL"
                     /locus_tag="VHR64_11170"
     CDS             complement(6728..8941)
                     /gene="purL"
                     /locus_tag="VHR64_11170"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX3303433.1"
                     /translation="MTTRTEIPRITPELWRSLALSDAEYHRIVELLGREPSVVELGMF
                     GSMWSEHCGYKHSRPLFPLLPTEAEFVVQGPGENAGAVDIGNGLAAVFKVESHNHPSA
                     VEPHQGAATGVGGIVRDIFTMGARPVAILDSLRFGPLDSERNRYLFENVVGGIGDYGN
                     CLGIPNVGGEVYFDESYNGNPLVNAMCVGVVEISQLMRARASGVGNVVMIIGAETGRD
                     GLHGATFASVEDPEASHRGVVQVGNPFLEKLLLEACLELLQGDDVVAMQDLGAAGLTS
                     SSVECAARGGVGIDIDVAKVPRREPGLTPFEVMLSESQERMLLVVRPDGVERVRAIVE
                     KWSLKSAGIGAVTETGNVVIRDGDQIVCDVPATYFTDECPTYVREAAEDPAIAAARAT
                     DWQDIPDIAPDRVAATTLELLGTENIGSRRSVYRRYDSTIMTNTVVGPGQSDAAVLRI
                     KGQETGLAISLDCNSRYCYLDPYRGAQLAVAEALRNVSCAGGRPLAITNCLNFRNPEK
                     PAGYFQLSRAVAGMADACRALGVVVVSGNVSLYNETADSAIFPTPTIGCVGVVDDVGN
                     HARTIWREGDQILLIGSGRPTLGGSEYLAALHGKTIGEPPVLDLDQEAAVQRVVRDSI
                     AAGEVRTAHDVSLGGLAIAIAEMAIHSGIGAMISLPADERCDNFWFGERASSILVAVE
                     TAQAAAVLDRAGAANMQATVIGTVMGSEVVYGPGDAIDLTTATERYESALTTGAV"
     gene            complement(8955..>9494)
                     /gene="purQ"
                     /locus_tag="VHR64_11175"
     CDS             complement(8955..>9494)
                     /gene="purQ"
                     /locus_tag="VHR64_11175"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX3303434.1"
                     /translation="GDYLRCGAIARFAPVMEPLKAFAERGGPVLGICNGFQVLIEAHL
                     LPGALLKNRSLTFHSDWVKLRVESGETAWTGSLTEGETVTFPVAHGVGNYFADPETLE
                     RIEGNGQVVFRYYNEDGESLESANFNGSVNSIAGICNAQRNVVGLMPHPERASDPLLG
                     PTDGLKMLGGIRTFLTVLA"
BASE COUNT         1641 a         3110 c         2996 g         1747 t
ORIGIN      
        1 cgaagccgag atccgcgaga ccgtgcgtcg gctgggcgtc tacgctgccg attgcctcgc
       61 cgagatcggc tgagcgccgt cgagatcaat gcggcccttg acgtagcgcg gggttaccat
      121 gccccgcttg gcagttcacc atgcgcaagt gacggagccg aacgactgtc cggatcggtt
      181 ttccgaaagg acgaagcgtc atcaggtccg ggcggggcgt ggtaaccccg cgctacgtcg
      241 gttctaccat caaccgttga acgtcgatca ggcggcggcg cggtttcgga aggagatgcg
      301 gcgctcggag ccgtggatga ggcgatcgat gttgccgcgg tggcgccaga cgatgatcgc
      361 cgcgatgatc acggcggcga tcgcgatcgg gtcggcgacg cggtcggaca gggccgcgat
      421 tgagagcgcg atgccgatgc tgactgccgt cacgatcgag cccaacgaca cgtagtgcgt
      481 cagcagtacg atcgcgacca tcaggaaaac ggcgaacgcg acccatggct gcaatgcgat
      541 caatgccccg gcgccggtcg cgacgccctt gccgccatcg aactcgatga acggtgacca
      601 gcaatggcca acgaccgtca gcacggccac cgcgccaacc agccacggtc cccgatcgag
      661 cgcgtgagcg atcaggaccg ggatggctcc cttcaggatg tcgaaaaaga gcaccagggc
      721 gctgatcttt gggccgtatg ctcgcagggc attggtggtg ccagttgagc cactgccgat
      781 cgtgcggaga tcagtgccgt aaaacagttt gccgagaatg acgccgacgg ggatggcacc
      841 ggccaggtag gcaaggacca ggaccgcgat gccgagcgga gtctccaaac tattcgtcct
      901 cttcccttcc ccggaacgcc atgcgcaccg ggttaccgac gaatccaaac tgatcgcgaa
      961 tctggttttc gaggtatcgc ttgtacgaga aatgtacgct ctgcgggtcg ttgcagaaga
     1021 agacgaaagt cggcggtgac acgtctgctt gcgtcgcata atagaacttc agccatttac
     1081 ccggcttgct ggccggtgcg tgggcggcaa cagcctcccg cagcagcttg ttcagcgctg
     1141 acgtcgagac gcgcttccgc cgctccatct cgaccagcag cgccatctcg agcacgttct
     1201 gaacccgctg gccgaacttg gccgagatga agacgagcgg cgcatagggc aggaaatcga
     1261 gctggcgctt cgcctcgtca cggtagaggt ccatcgtcga ggaatccttc tcgatcaggt
     1321 cccacttgtt gacgatgacg acgatgccct tcttctgctc ttcgacatag ccggcgatat
     1381 gcagatcctg cgcggtgaag ccctcggtgg aatcgacgac caacaggacg acatccgacc
     1441 ggtcaatcgc gcgcatcgag cggagcacgc tgaagtgctc gatcccctgc tcgatccggc
     1501 ctcggcggcg aatcccggcg gtgtcgatca gcgtcaacgg gcgaccgttc cactccatgt
     1561 gcgtgtcgag actgtcgcgg gtggtgcctg gcacatcgct gacgattgag cgctcctcgc
     1621 cgatcagcgc attgaccagc cggctcttgc ccacattcgg tcggcccacg atcgcgatct
     1681 tgggtccggc aatctccggt tcttcctcga agtgcggcag gttctcgacg atgcggtcca
     1741 gcagatcgct ggtgccgacg ccgtgatatg acgacatcgc catcggatcg ccaaggccga
     1801 gctcgtagaa ctcgaccgcc atgtccttgc ggttgatgtt gtcgaccttg ttggcgacga
     1861 ggatggtcgg cttgtcggtg cgacgcagca tgtcagcgac ttcatgatcg ccggccgtca
     1921 tcccggcctt ggtatcgacc atgaagacga tcacgtccgc ctcgtcgatc gcgctgcggg
     1981 cctgatcgcg cgtcatctgg gcgatctcgg cggtcgacga cgcttcgatc tcggacgggt
     2041 cctggagacc ggccgtgtcc acgatggtga actcgacgcc accccaggtc gtcgtgccgt
     2101 agagccggtc gcgcgtcgtg ccggggatat cctcgacgat ggcccggcgc tcgcccacga
     2161 gtcggttgaa gagtgttgat ttgccgacgt ttggacgtcc gacgatcgcg actataggtc
     2221 gtgccacagt ttcaccagca tgtcgtaaag gcgttcacag attagagaca gcgccggact
     2281 ccgatcggca tgcttgctgt cgttcgggct acgaaagact cgggagatga gtatagcaaa
     2341 cgattgtgcc gatccccgct tttcgcagcc cgacctttct cctagatttc gcgaagcgcc
     2401 ccgatcagcc ggtcaatggc cgcgcgatcg ttcaactcgg tcgcgcacag gagccagtgg
     2461 cccgccaggg cctcctcgta gcggccaagg tccagaccgc cgatgatgcc gtgctcggcc
     2521 agcgtgcgat tgagctccgc cggcgccatc ggagtcttga ccacgaactc gtggaagaac
     2581 tcgccgtcac ccgcgcgctc gtaccccgga agcgacgtga cctgatcggc caggtagtgc
     2641 gcgttctggt agcaacgctc ggcgacttcc ccaaggccct gcggcccaag cgccgacatg
     2701 tagatagtcg ccgcggtggc catcaggccc tggttggtgc agatgttgct cgtcgccttc
     2761 tcgcggcgga tgtgctgctc gcgcgtctgc agcgtcagca cgaacccgcg cttgccctcg
     2821 gaatcggtcg cgactccggc caaacgaccc ggcatctggc gaacgaagct cttgcgcgtg
     2881 ccgagtaagc ccacatacgg gcccccgaag ctctgggcga cgccgagcga ctggccctcg
     2941 gcagtcacaa aatcggcgcc catctcgcct ggaggagtca ggacacccag cgggaccggc
     3001 agtgtcgaga cgatcaacag ggcgcccgct tcgtggaccg cctcggcgac cgcgcgcacg
     3061 tcttcaatgc cgccgtagaa gttcgggtac tggatgacga cgcaggccac atcgtctcca
     3121 agctcggcct tgaccgcgtc tacgctcgtg agcaggctcg acgcgtcggg cagcgggacc
     3181 tccgtgattt cgagatgccg gccgcttacg tacgtttgga gcacctcgcg gtaatgcggg
     3241 tggacggtgc tcgcaattac gatccgctcg cgcttcttga ccgatgcgat cgccatcagg
     3301 accgcctcag cgaccgccgt tgcgccgtcg tagagcgagg cgttggcgac ctccgtcccg
     3361 agcagcgcgg cgaccatgct ctggaactcg taaatgacct gcaacgtgcc ctgcgccact
     3421 tcgggctgat agggcgtgta ggccgtaaag aactcgccgc gcagaagcag ctgattgacc
     3481 agtgccggca cgtagtggct gtagctgccc gcgccgagga agctgttgcc cggcgcgatc
     3541 tggaggttct tgccggccag ctctgtcaac cgggcggccg cttccatctc ggtcaacgcc
     3601 ggcggcaaat gcaggtccgg acggcggata tcctcaggaa tgcctgagaa cagctcctcg
     3661 accgaggcga cgccgaccgc ggctagcatc tcggcgcggt cctgttcagt atgtgggttg
     3721 aatgtcatgg acggagagac cggaccttcc tgcgactagt gcgcgacttc ttcgaggtac
     3781 ttctcgtact catccggcga catcaggttg tcgagcccgc tctggtcacc cacgcggatc
     3841 ttgaccagcc acgcctgctc gtatggcgac gcattgacga cttccggccc gctgaccgcg
     3901 ccctcattgc gctcgacgat ctcgcccgtc accggggcat aaatgtcgga cgccgccttg
     3961 acggactcga ccacgccgaa tggctcgtcg gcgctgatcg actcgcccgg ctccggcaat
     4021 tcaaggtacg tgatgtcgcc gagctcgccc tgcgcgtagt cggtcatgcc catcgtcacg
     4081 atgtcgccgt caactcgggc ccactcgtgc gatttcgtgt acttcagatc cccgggtacc
     4141 gccatttgtg cctttcctct cttgctgtta ttgaccggat cgcttgtaaa acggcatctt
     4201 gatctgctcg gcgttgatcc acttgccgcg aatctcgacc tgcagtggct tgccgacgcc
     4261 ggcatattcc ggcgcaatca gcgccagccc gatattctcg cctaatgtcg gggagaatgc
     4321 accgctcgtc acctcgccga tcacgttacc gtcgatgccg accgggtagt gcgttcgtgg
     4381 cgcgccgctg cgctcggtca tgcgaaaccc gaccgccttg cgcttcggtc cttcggattt
     4441 cacctgggcc atgccctccc gcccgacgaa atcgcccttg ttgagcttga cggcccagcc
     4501 gagccccgcc tcgtacgggg agatgtcgtc gccgagctcc tgaccataga gcggcatccg
     4561 cgactcgagt cgcaacgtgt cgcgcgcgcc gagaccgatc ggttgcaggc cgtgctcctt
     4621 gccggcctcc atcagcacgt cccagatcgt gccggccgtg tcgatcgggc cgtagatctc
     4681 gaacccatcc tcgccggtgt acccggtacg agcaatcatg accggaatcc cggcaaccgt
     4741 cccggagcgc cagtggtagt gcgcgatatc tgaaaggtca tcgccggaga tcgactgggt
     4801 tatcgcggcc gcatttggtc cctggatcgc gatcatcgcg gtctcgtcgg agatgtcgcg
     4861 gaccgtgacg ttcaggtctt cgagtctttc gcgctgctct tgcatccagg caacatcgcg
     4921 gaaccggttc gaggcattga cgacgaccat gtaatcgccg ccctctggcc ggcggtaaat
     4981 caggatgtcg tcgattacgc cgccgtgctc gttcggcaac atggcgtatt gcgcttcgcc
     5041 gggttgcagg gcgctgacat cgttcgtcgt cgtggcttgc aggtatgcga gcgcatccgg
     5101 ccccgtcacg tcgacctgac ccatgtggcc gagatcgaag agcccggcct gattccgaac
     5161 cgcgttgtgc tcggcgatga tgcccgagta ctggaccggc atctcccaac cggcaaacgg
     5221 aatgatcctc gccccaagcg ccacatggcg atcaaagagc ggggtgcgcc tcaacgtgtc
     5281 gtctgccacc gtgctccctt tgctagaccc gttccagaac caatttgtcc aggcccatct
     5341 ggacgttgac cgggtaattt ccggtcaggc agccggtgca gaatcccgcg tccggcgcct
     5401 tgattgcttc cagcaatcct tcaagcgaaa gatacccaat cgagtcggct ccgatgaacc
     5461 ggccgatctc cggaacagtc atccgggcgg cgatgagttc gtcccgcctg gcgagatcca
     5521 cgccgaggta gcacggccac atcatcggcg gcgcgtggac ccgcatgtgg acctctttgg
     5581 cgccggcatt ccggagaagc tgcacgatcg gccggctggt cgtgccgcgc acgatcgtgt
     5641 cgtcgaccag gaccacccgc ttgccggcga tcgcttcggg cagcgcattg aacttgagcg
     5701 agacgccctg gtcacgcagg cgctgatcgg gctgaatgaa ggtcctgccg atgtaccgat
     5761 tcttgatcag cgcctcggcg tacggtatcc cgctctcctg cgcgtacccg atcgcggccg
     5821 gcgtcgccga atcgggcagg ccgaccacga cgtccgcttc gatcgggaac tcgcgcgaga
     5881 gctgcgcacc catcctctgg cgcacctgat gcaggcgctg gccgtgaatc aggctgtcgg
     5941 gtcgtgcgaa atagatcagc tcgaacaggc acatggcctg cttgcggggg aattcctgcg
     6001 tgtggctgtt cgcagttgcg cccttgatct cgacgatctc gcccggctcg acctcccgca
     6061 cgaacgtcgc gccgacggtc gccagtgcgc aactctcgga ggcgacgacc cagttgtcgt
     6121 cgatcttgcc gaggcagagc ggccgcacgc cgagcggatc gcgaaccgcg aagaggcggt
     6181 ccctggtcag aatcgcgagc gagtacgcgc ccagcatgcg ggtcatcgcg ttgcggatct
     6241 tgtcgacgaa gctgaagccg gggctgagcg cgatcaatcg agtcaggatc tcgctatctg
     6301 tggtggtcag gaatcgctcg ccctcatgct ccagctcctc gcgcaagttc agggcattgg
     6361 tgacgttgcc gttgtgcgcg accacgattg gaccgaggtt cgagccgctc accatcggtc
     6421 cggcattgcg cgggctgttc gacccttgtg tcgagtaccg ggtatggccg acggcgatgt
     6481 gaccgggcag gagggcgagg tcgtcctccg agaacgcctg gctgacgagt ccgattcggg
     6541 tccgaatcga gacgtgctcg ccgtcggagg tggcgatacc ggcgctctcc tggccgcgat
     6601 gctgcagcgc gtagagaccg aagaaggtga tccgggcgac gtcctgctcc ggcgcgaaaa
     6661 tgccgaatac accgcattct tcggttggct tgtcgtccca gatgtcttcc ggcatctccc
     6721 cgccttccta caccgcgccc gtggtcaggg ccgattcgta tcgctccgtc gccgtagtca
     6781 gatcgatcgc atctccgggt ccgtagacga cctccgaacc catcaccgtg ccgatcactg
     6841 tggcctgcat gttcgcggcg ccggctcgat cgagcacggc cgcggcctgg gccgtctcga
     6901 ccgcgaccag gattgagctc gcgcgctcgc cgaaccagaa gttgtcgcac cgctcgtcag
     6961 cggggagcga gatcatcgcg ccgatcccgg aatggatcgc catctcagcg atcgcgatcg
     7021 ccaggccgcc cagcgaaacg tcgtgggctg tccggacctc gcccgccgcg atcgaatccc
     7081 gcaccacccg ctgcaccgcc gcctcctgat cgagatcgag gaccggcggc tctccgatcg
     7141 ttttcccgtg cagggcggcc aggtactcgg atccgccgag cgtgggccga ccgctgccga
     7201 tgagcaggat ctgatctcct tcgcgccaga tcgtccgcgc atggttcccc acgtcgtcga
     7261 caacacccac gcagccgatt gtcggcgtcg ggaaaatggc cgagtcggcg gtttcgttgt
     7321 agagagagac gttgcccgag acgaccacca cgccgagcgc cctgcaggca tcggccatgc
     7381 cggccacggc tcgactgagc tgaaagtagc cggccggctt ctcgggattg cggaagttga
     7441 ggcagttggt gatcgcgagc ggcctgccac cggcacaact gacattgcgg agcgcctcgg
     7501 cgactgccag ttgagcgccg cgatatgggt cgaggtagca gtatcgcgag ttgcagtcga
     7561 gcgagatcgc gaggcctgtc tcctgaccct tgatccgaag cacagcggca tccgactgac
     7621 ccggtccgac aacggtattg gtcatgatcg tggagtcgta gcgccggtag accgatcgtc
     7681 gactgccaat attctccgtg ccgagtagtt ccagcgtggt ggctgccacc cggtccggcg
     7741 caatgtcggg aatgtcctgc caatcagtag cgcgcgccgc ggcgatcgcc ggatcctcgg
     7801 ccgcctcgcg aacataggtc gggcactcgt cggtgaagta cgtggccgga acatcgcaga
     7861 cgatctgatc gccgtcgcgg atgacgacgt tgccggtctc tgtgaccgcg ccgatcccgg
     7921 cggacttcag cgaccacttc tcgacgatcg cccgcactcg ttcgacgcca tccggccgca
     7981 cgacgagcag catccgctcc tggctttcgc tgagcatcac ctcgaacggc gtcaggcctg
     8041 gctcgcgacg cgggactttc gcgacatcga tgtcgatgcc gactcctccc ctcgctgcgc
     8101 actccaccga gctgctggtc aatccggcgg cgccgaggtc ctgcatagcg acgacgtcat
     8161 ctccctggag cagctcgagg catgcctcca ggagcagctt ctcgaggaac ggattcccga
     8221 cctgcaccac gccgcggtgc gatgcctcgg ggtcctcgac cgacgcgaac gtggcgccgt
     8281 gcagtccgtc tcggccggtc tcggcgccga tgatcatgac gacgttgccg acgcccgagg
     8341 cgcgggcccg catgagctgc gagatctcga ccacgccgac gcacatcgcg ttgacgagcg
     8401 ggttgccgtt gtacgactcg tcgaaataga cctcgccgcc gacgttcggg atgcccaggc
     8461 agttcccgta atcaccgatt ccgccgacca cgttttcgaa gaggtagcga ttgcgctcgg
     8521 agtccagcgg cccgaaccgg agcgagtcga ggatggcaac cggccgcgcg cccatcgtga
     8581 agatgtcgcg aacgatgccg ccgacacccg ttgccgctcc ctggtgcggc tcgacggcgc
     8641 tcggatgatt gtgcgattcg accttgaaga cggccgcgag accgttgccg atgtcgaccg
     8701 ccccggcatt ctcgcccggt ccctggacga cgaattcggc ttcggtcggg agcaacggaa
     8761 agagcggccg cgagtgcttg tatccgcagt gctcgctcca catcgagccg aacatgccca
     8821 gctcgacgac cgacggctcg cggccgagaa gctcgacgat cctgtggtac tccgcgtcgc
     8881 taagggcgag gcttcgccaa agctctggtg tgatccgagg aatttcggtg cgggtcgtca
     8941 taggtccgga gctcctaggc cagtaccgtg aggaatgtgc ggatcccgcc caacatcttc
     9001 aacccgtcgg ttggtccgag taacgggtcc gacgcgcgtt cgggatgggg catcaggcca
     9061 accacattgc gctgcgcgtt gcagatgccg gcaatcgagt tcaccgaacc gttgaaattg
     9121 gcggattcga gcgactcgcc gtcctcgttg tagtagcgga agacgacctg gccgttgccc
     9181 tcaatgcgct cgagggtttc ggggtcggcg aaatagttgc cgacaccgtg cgcaaccggg
     9241 aaggtcacgg tctcgccctc ggtcagggaa ccggtccagg cggtctcacc ggactcgacc
     9301 cgcagcttga cccagtcgct gtgaaaggtc agagatcggt tcttgagcag agcgccgggg
     9361 agaagatgcg cttcgatcag cacctggaag ccattgcaga tgccgaggac tggtccgcca
     9421 cgttcggcga acgccttcag cggctccatg accggggcaa agcgggcaat cgcgccacag
     9481 cgcaggtaat cgcc
//
LOCUS       DATAOZ010000436         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4119758, whole genome shotgun sequence.
ACCESSION   DATAOZ010000436 DATAOZ010000000
VERSION     DATAOZ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4119758"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VHR64_11180"
     CDS             <1..1053
                     /locus_tag="VHR64_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3303435.1"
                     /translation="PDVFIATGLGVGELSAAGYLEPLDGYLGGWDGWAQYPDVVRRAL
                     TYQNQVWALPCSIDTHFFYYRKDLLEKAGLAREWQPASPDDILATARNLKSALPDVMS
                     YGIYAGANGGNGTAVRGFLPLVYAFGGSLTDETGRWIIDSPALRGALAHYETAFQVDK
                     TAPGEIMTSVNPPRMMRTSIATGEMAMLYDGSWVYADWDAAVPDITRQQIGYTQYPTA
                     DGSPPFAVGGLGNSWYINVKSGAKDLAWTLLTAGNVPEVLTALNVASPHIPPRLDAAG
                     ADAFKATEFHTAMVGSISNLIIAPPDPAYRQLIGVIQNATGIVATGEATPEEALKKYA
                     DELTRILGEDRVISQS"
     gene            1190..2047
                     /locus_tag="VHR64_11185"
     CDS             1190..2047
                     /locus_tag="VHR64_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3303436.1"
                     /translation="MPRLAAITPAAVLLSLFFVGPALWAVYASLTDRTLIGIGAQDTS
                     FIGLDNYRRLWDDPDFIKILRNTMMFVVGSAVIGQTGLGLLLALLINQARGSRLAGLA
                     YAAVLVAWISPPTFAGSIWGSIVDPKHGTLNQVLSAFGLPKVAMLDNHAMLLVVIADV
                     WRGTAFAMIIFLGALQTIPPQIYEAARVDGANAWRRFRDHTLPMIGHLCAIVLMTTTI
                     TTMGSFLLIVILTNGEPGKGTETIALYAFHRAFSTYLIGYGAAISVVMLGLNLLFAAA
                     YLRIARVSE"
     gene            2044..2907
                     /locus_tag="VHR64_11190"
     CDS             2044..2907
                     /locus_tag="VHR64_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3303437.1"
                     /translation="MITNPATFPISARRRRQVRSLAGWILLRAALLLVTVAFGLPFLW
                     TVASALDASDASGLPWPRDVTLSHFQALFDDLDVATGLRNSLIVSLSTMVLATITAAL
                     AGYGLSRLESRRKTWLIYGVLLLQTIPLAVTMVPIYDLSVRLDLQNSYRGLILTHSAI
                     ALPFLVWLMKGFTDAVPRTMEEEAWVDGASELRAWFDVVVPATLPGLAIVAGFSFINA
                     WSEVLMVVLLVTDSKLETLPFKFYDVASGSIEVHQTAALGVLYVLPALILFLALRRLF
                     VRGIFESAQGL"
     gene            complement(2923..3861)
                     /locus_tag="VHR64_11195"
     CDS             complement(2923..3861)
                     /locus_tag="VHR64_11195"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX3303438.1"
                     /translation="MAAKRNKVIITCAVTGAVHVPSQSEYLPITPEQIADNAIGAAEA
                     GAAILHLHARNPENGRPTPDPDMFMQFLPEIKERCDAVINITTGGGQGMTLEQRLAAA
                     MRAKPEMCSLNMGSMNFGRFGIANRIKEFRFDWEADDLEASRDYIFRNTYKDIAYILR
                     ELGETHGVKFEFECYDVGHLYNLAYFVDEGLIKPPFFVQTIFGILGGIGTDVEDLLHM
                     KRTADRLFGDAYQWSILGAGRHQINLVTMGAIMGGNVRVGLEDNIYLGKGELAKNNAE
                     LVAKTARILGELSLEIATPDEAREVLQLKGVHNVGF"
BASE COUNT          669 a         1277 c         1179 g          804 t
ORIGIN      
        1 cccgatgtgt tcatcgccac aggtctcgga gtcggtgaac tgtcggccgc cgggtacctc
       61 gagccactcg atgggtacct gggaggctgg gacggctggg cgcagtatcc ggacgtcgtt
      121 cgccgcgccc tcacctacca gaatcaggtc tgggcattgc cgtgcagcat cgacacgcac
      181 ttcttctact accggaagga tctgcttgag aaggccggcc tcgcgcggga atggcaaccg
      241 gcatcgccgg atgacatcct cgccacggcc cgtaacctca agtcggcgtt gcccgatgtc
      301 atgtcgtacg gcatctacgc gggcgcaaac ggcggcaacg gcaccgcagt tcgcggcttc
      361 ctgccgttgg tctacgcctt cggcggatct ctgaccgacg aaacgggccg ttggatcatc
      421 gacagtccag ccctccgtgg agcgcttgcc cactatgaga ctgcgttcca ggtcgacaag
      481 acagcgcccg gtgaaatcat gacgtcggtg aatccgcccc ggatgatgcg gacatcgatc
      541 gcgaccggcg agatggcgat gctctacgac gggtcgtggg tatatgcaga ttgggatgca
      601 gccgttccgg atatcacgcg ccaacagatc ggctataccc aatatccgac cgctgacggg
      661 agcccgccgt tcgcagttgg cggacttggt aatagctggt acatcaatgt caagtccggc
      721 gccaaggatc tggcttggac attgctcacg gccggtaacg tgccggaggt cctgaccgct
      781 ctgaacgtcg ccagcccaca catcccgcca cggcttgacg ccgccggagc tgatgcattc
      841 aaggcaactg agttccatac ggccatggtt ggcagcatct cgaacctgat catcgccccg
      901 ccggatccgg cctatcgcca attgatcggc gtgatccaga acgcgaccgg aatcgtcgcg
      961 actggcgagg ccacaccgga ggaggcgcta aagaaatatg ccgacgagct tacgcgcatc
     1021 ctcggtgagg accgggtcat ctcgcagtcg taggggagca accgtggcga tcgcaacgcg
     1081 ccaaacgcag cctcaaccac cgctgagtga gcccggagcg ggcagtcgac tgccgactgc
     1141 ccgctccgct ccctgggcgg gatcgcggca ctcttcactt cggcgggaat tgcctcgtct
     1201 tgcagcaatc acgccggcag cggtcctcct gtcgctcttc ttcgtcggcc ctgcgctctg
     1261 ggccgtctac gccagcctga ccgatcgcac cctgatcggt atcggagcac aagacaccag
     1321 tttcattggc ctcgacaact atcgccggct ctgggacgac cccgacttca tcaagatcct
     1381 ccgtaacacc atgatgttcg tcgttggctc ggccgtgatt gggcagactg gcctcgggct
     1441 cctgctggcg ctgctgatca accaggccag agggagtcgg ttagcgggcc tcgcttacgc
     1501 cgcagtgctg gttgcatgga tcagcccgcc gacgttcgcc ggatcgattt ggggcagcat
     1561 cgtcgatccc aagcatggca cgctgaatca ggtgctctcg gcgttcggtc tgcccaaggt
     1621 cgccatgctg gacaatcacg cgatgctcct cgtcgtgatc gccgacgttt ggcgcggaac
     1681 ggcgttcgcg atgatcatct tcctcggggc actccagaca atcccccctc agatctacga
     1741 ggcagcgcgc gtcgacggcg caaacgcctg gcgccggttt cgcgatcata cgctgccgat
     1801 gattgggcat ctctgcgcca tcgtgctgat gacgaccaca atcacgacca tggggtcctt
     1861 cctgctgatt gtcatcctga ccaatggcga acccggcaag gggacggaga ccatcgcgct
     1921 ctacgcattc catcgcgcgt tctcaacgta cctgatcggg tacggcgcgg cgatctcggt
     1981 ggtcatgctc gggctgaatc tactctttgc cgccgcctat ctccggatcg cgagggtttc
     2041 ggaatgatca ccaacccggc caccttcccg atatccgcac ggcgacgaag acaggtccgg
     2101 tcgctggctg gctggatcct gctccgggcg gcgctgttgc tcgtaacagt ggccttcggt
     2161 ttgccgtttc tctggaccgt cgccagtgcg ctcgacgcat cagatgcgag cggcttgccc
     2221 tggccgcgag atgtcacgct ctcgcatttc caggcgctct tcgacgatct cgacgtggcg
     2281 accgggctgc gcaacagcct gatcgtgtcg ctctcgacga tggtcctcgc aaccatcacc
     2341 gcagcactgg ccgggtacgg tctgtcccgc ctggaaagcc ggcgcaagac ctggctgatc
     2401 tacggagtgc tgctcctcca gacgatccca ctcgccgtca cgatggtccc gatctacgat
     2461 ctctcagtgc ggctcgacct ccagaactcc tatcggggcc tgatcctgac ccactctgcc
     2521 attgcactgc cattcctcgt ctggctgatg aagggattca ccgatgcggt acctcggacg
     2581 atggaagagg aagcctgggt cgatggggca tccgagctgc gtgcctggtt cgacgtggtc
     2641 gttccggcga cgctgccggg gctcgccatc gttgccggat tctcgttcat caacgcctgg
     2701 tccgaggtgt tgatggtcgt gctgctcgtg accgattcca aactggagac gctcccgttc
     2761 aagttctacg acgtcgccag tggcagtatt gaggtgcatc agaccgcagc gctcggcgtg
     2821 ctttatgtgc tgcctgccct gatcctgttt ctcgcgcttc gtcgactctt tgttcgcggc
     2881 atcttcgaga gcgcccaggg cctttagcgg cgtgccaagg ctctagaacc cgacgttgtg
     2941 cacgcccttg agctgcaaga cctcccgcgc ttcgtccggg gttgcgatct ccagcgacag
     3001 ttcacccagg atccgggccg tcttggcgac caactccgcg ttgttcttcg ccagctcgcc
     3061 cttgccgagg tagatgttgt cctccagccc gacccgcacg ttgccgccca tgattgcgcc
     3121 catggtgacc aggttgatct ggtgccggcc cgccccgagg atcgaccatt ggtacgcgtc
     3181 gccgaagagc cgatcggccg tccgtttcat gtggagcagg tcctcgacgt cggttccgat
     3241 gccgcccagg atgccgaaga tcgtctgcac gaagaacggc ggcttgatta gcccctcatc
     3301 cacgaaatag gcaaggttgt agaggtgacc gacgtcgtag cactcgaact cgaacttgac
     3361 gccgtgcgtt tcgcccagct cgcgcaggat gtaggcgatg tccttgtacg tgttgcggaa
     3421 gatgtagtcg cgcgaggcct cgagatcgtc cgcttcccag tcgaaccgga actccttgat
     3481 ccgattggcg atcccgaagc ggccgaagtt catcgatccc atattcagcg agcacatctc
     3541 cggctttgcc cgcatcgccg cggccaggcg ctgctccagc gtcatgccct gcccgccgcc
     3601 ggtcgtaatg ttgatcaccg catcgcaacg ctctttgatc tctggcagga actgcataaa
     3661 catgtcggga tcgggtgtcg gccgcccatt ttcaggattg cgcgcatgca ggtggaggat
     3721 cgccgcgcct gcttcggcgg ccccgatcgc gttatcggcg atctgctccg gcgtgatcgg
     3781 caggtactcc gactgtgagg gaacatgcac ggctcccgtg accgcgcagg tgatgatgac
     3841 cttgtttcgc ttggcagcca acgcgttcgc tcctcgtggt tagtggtcga ttgatcagca
     3901 atcgtcactc taccgcaggg atcgcgagg
//
LOCUS       DATAOZ010000437         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2018113, whole genome shotgun sequence.
ACCESSION   DATAOZ010000437 DATAOZ010000000
VERSION     DATAOZ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018113"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VHR64_11200"
     CDS             <1..581
                     /locus_tag="VHR64_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303439.1"
                     /translation="PITGRTQVGKINQADGYDLAQKIFADKISIGGGNFSVSIFWWIG
                     LTILATWLLLRSSFGNWTFGVGGSFQAARNVGVPVHRVKILLFMGTAASAWLVAMIQA
                     ITFTGTDVLRGTYREFYTIIAVVVGGTLLTGGYGTVVGAFFGALIVGMVNQGIVFAGF
                     DADWFQVFLGAMLLAAVLVNRFIRQRATGERS"
     gene            578..1435
                     /locus_tag="VHR64_11205"
     CDS             578..1435
                     /locus_tag="VHR64_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3303440.1"
                     /translation="MSAMPADATLPDVTPILEVRNVSRYFGSVIALKDVSMSVYPGEV
                     MCLLGDNGAGKSTLIKILSGVHQPSEGEYLVEGTPVHFTSPRDALAAGIATVYQDLAM
                     IPLMSVSRNFFLGSEPQVGWGPFRRFDFKTADRVTRDELSKMGIQIRSTDQAVGTLSG
                     GERQSVAIARAVHFGAKVLILDEPTAALGVKEAGVVLRYIAQARARGLGVVFITHNVH
                     HAYPIGDRFTLLNRGTSYGTFAKSEVTREEVVSMMAGGEELDELAHELEEFARTDHAR
                     TVRIVPDQP"
     gene            1432..2319
                     /locus_tag="VHR64_11210"
     CDS             1432..2319
                     /locus_tag="VHR64_11210"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3303441.1"
                     /translation="MTAIKVGTAPVNWNNSDVPDWRTPTPFPQILDEMASAGYAGTEH
                     DRQFPDAPATLKAALDQRGLALCASYQWVHFSHADGFAQDLTAIDSILDMLTSVGCDV
                     LVVADAMTPERIALAGRVPEDGSAGLDGAGWQRFAANLTTVAGRAAARGVRTCYHNHV
                     GSHVESPAECARLVELLPGTGATLCFDTGHYAYGGGDPTKFVREQSALIGHLHLKDVD
                     PGILARAREQGWGFTDALRQIVFCPFGSGMVDIPAIVRALQDVAFSGWVVVEQDTCEG
                     DPTERAAAHRAYLRSCCGI"
     gene            2309..>2639
                     /locus_tag="VHR64_11215"
     CDS             2309..>2639
                     /locus_tag="VHR64_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303442.1"
                     /translation="MESSAAIEPRDSQTMSLDALSARTRGLILESIHHAGAGHVGGPL
                     SATDLLVHLYFRTLNIDPTRPDWPERDRFILSKGHSSIALYTVLALRGYYPVEELRTF
                     DAIDSRLQ"
BASE COUNT          417 a          934 c          840 g          448 t
ORIGIN      
        1 gcccgataac cgggcgcaca caagtcggca agatcaacca ggccgatggc tacgacctcg
       61 cccagaagat cttcgccgac aagatttcga tcggcggcgg caacttctcg gtcagcatct
      121 tctggtggat tggcctgacg attctcgcga cctggctgtt gctgcgctcg tccttcggca
      181 actggacctt cggcgtcggc ggcagttttc aggccgcgcg caacgtcggc gtgccggtcc
      241 accgggtcaa gatcctgctc ttcatgggca cggccgcctc cgcctggctg gtcgcgatga
      301 tccaggcgat caccttcacc ggtaccgatg ttctgcgggg gacataccgc gagttctata
      361 cgatcatcgc ggtggtcgtc ggcgggacgc tgctgaccgg cggctatggc accgtggtcg
      421 gcgccttctt cggcgcgctg atcgtcggca tggtcaacca gggcatcgtc ttcgccgggt
      481 tcgacgccga ctggttccag gtcttcctgg gcgcgatgct gctcgcggcc gtgctggtca
      541 accgcttcat tcgccagcgc gcgacaggag aacggtcatg agcgcgatgc ccgccgatgc
      601 gacattgccg gacgtgacgc cgattcttga ggtgcgaaac gtctcgcgct atttcggctc
      661 ggtgatcgct ctcaaggacg tctcgatgtc ggtctacccc ggcgaggtca tgtgcctgct
      721 gggcgacaac ggcgccggca aatcgaccct gatcaagatc ctctccggcg tccaccagcc
      781 cagcgagggc gagtacctag tcgaaggcac gccggttcat ttcacgtcgc cccgcgacgc
      841 gctcgcggcc gggatcgcca cggtctacca ggacctagcc atgatcccgc tgatgagcgt
      901 ctcccggaat ttcttcctcg gctccgagcc acaggtcggc tggggaccgt tccgccgctt
      961 cgacttcaag acggccgacc gggtcacgcg ggacgagctc tcaaagatgg gcatccagat
     1021 ccgcagcacc gatcaggccg tcggcaccct ctcgggcggc gagcggcaat cggtcgcgat
     1081 cgcccgggcg gtccatttcg gcgcgaaggt cctgatcctc gacgagccga ccgcggcgct
     1141 gggcgtgaag gaggccggcg tcgtgctccg ctacatcgcg caggcacggg cgcgcggcct
     1201 gggcgtggtc ttcatcacgc acaacgtcca ccatgcctac ccgatcggcg atcgcttcac
     1261 gctgctcaat cgcggcacgt cctacggcac gtttgccaaa tcggaggtga cccgggaaga
     1321 ggtcgtctcg atgatggccg gcggcgagga gctggacgag ctcgcccacg agctggaaga
     1381 gttcgcccgg accgatcacg cccgaacggt ccggatcgtc ccggatcagc catgaccgcg
     1441 atcaaggtcg gcaccgcgcc cgtcaactgg aacaacagcg acgtacccga ctggcgcacg
     1501 ccgacgccct tccctcaaat cctcgatgag atggcctcgg ccggctacgc gggcaccgag
     1561 cacgaccgcc agttcccgga cgcaccggcc acgctcaagg cggccctcga ccaacgcggg
     1621 ctggccctct gcgcctccta tcagtgggtc cacttcagcc acgccgacgg cttcgcgcag
     1681 gacctgaccg cgatcgacag catcctcgac atgttgacat ccgtcggctg cgacgtgctc
     1741 gtcgtggccg atgcgatgac gcccgagcgg atcgcgctgg ccgggcgggt cccggaggac
     1801 ggtagcgctg gtctcgacgg ggccggctgg cagcggttcg ccgccaacct gacgaccgtc
     1861 gccgggcgcg cggcagcacg aggcgttcgc acctgctatc acaaccacgt cggcagccat
     1921 gtcgagagtc cggcggagtg cgcccggctg gtcgagctcc tgcccgggac cggtgcgacc
     1981 ctctgcttcg acaccggcca ctacgcctac ggtggcggcg acccgaccaa gttcgtccgc
     2041 gagcagagcg cgctgatcgg tcacctccat ctcaaggacg tcgaccccgg catcctcgcc
     2101 cgtgcccggg agcagggctg gggcttcacc gacgccctcc gccagatcgt cttctgcccg
     2161 ttcgggagcg ggatggtcga tatcccggcc atcgtgcgcg cgctgcaaga cgtagcattc
     2221 agtggctggg tcgtggtcga gcaggatacc tgcgaaggcg atccgaccga gcgcgccgcc
     2281 gcccatcgcg catacctacg gagttgttgt ggaatctagc gccgccatcg aaccccgcga
     2341 ttcgcaaacc atgtcgctcg acgcattgag tgcccgcacc cggggcctga tcctcgagtc
     2401 gatccaccac gccggcgccg gtcacgtcgg cggtccgctc tcggcgaccg acctgctcgt
     2461 ccacctctat ttccggacgc tgaacatcga tccgacgcgg ccggattggc cggagcgcga
     2521 ccgcttcatc ctgagcaagg gccactcctc gatcgcgctc tacaccgtgc tcgccctgcg
     2581 cggctattac ccggtcgagg agctgcgcac cttcgacgcg atcgactccc gcttgcagg
//
LOCUS       DATAOZ010000438         4295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2018188, whole genome shotgun sequence.
ACCESSION   DATAOZ010000438 DATAOZ010000000
VERSION     DATAOZ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018188"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..620)
                     /locus_tag="VHR64_11220"
     CDS             complement(99..620)
                     /locus_tag="VHR64_11220"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3303443.1"
                     /translation="MTEPIIDEQGRTEPPRSGGPVEVVMGFHRFLRETLLWKCAGLTE
                     EQLRWSPVPSGTCLLGILKHSVNVERWWIAQVIGGLTVEFDWTDDDKDADWRPSPDDT
                     FESIKAAYDAEAERSAQILAGVNWDDAPKEAEAAAHGLNVGWVLTHMVEEVGRHCGHA
                     DLIRELVDGQTGE"
     gene            complement(740..1246)
                     /locus_tag="VHR64_11225"
     CDS             complement(740..1246)
                     /locus_tag="VHR64_11225"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3303444.1"
                     /translation="MDDLTRPQPPRAADPVTLTYAFHVFSRETMIRKLEGLTDAAQRR
                     IALPSGATMLGLFKHCIHVERLWIAYAMGGLDVDFPWTEEDEEADWKVGPDESFESLV
                     ALAREEAARSDAIIPTLKWDEIGKSQRSLEEGLTVGWILTHLNWEIARHLGQMDVMRE
                     LADGVTGE"
     gene            complement(1259..2194)
                     /locus_tag="VHR64_11230"
     CDS             complement(1259..2194)
                     /locus_tag="VHR64_11230"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX3303445.1"
                     /translation="MGDAAPAKQYTERSAEAVRARRVALPPLIRAMRPLQWSKNSLVF
                     AALLFDKRVFQWDALWHTLAAAMVFSAVSSAIYLVNDVRDLHQDRLHPKKRNRPIAAG
                     EVSPGQAMRVAVALLTVGLITAVAVRPEFAIVIVAYIALMLAYSYGLKRMVILDVFAI
                     AAGFVLRAVGGAVAISVPASPWLYVCTALGALFIGFGKRRNEIVTLEAAAGHHRANLE
                     EYSLPMLDQIIAIVSAAMLIAYSLYTFDAANVPDSHAMMLTIPFVAYGLFRYLYLIYR
                     RGEGGSPEVLLIKDPGLISCIAGWVATSLIILYLS"
     gene            complement(2206..3270)
                     /locus_tag="VHR64_11235"
     CDS             complement(2206..3270)
                     /locus_tag="VHR64_11235"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX3303446.1"
                     /translation="MATDSSIRPRRVLITGGAGFLGINLVRYLLERNVNVTSLDIAPF
                     DYPDVRDKVRIVTGDIRDKSAVELALEDVDMVVHCAAALPLYSPEDIYTTDVVGTRVV
                     MTTALQRGIARVVHISSTAVYGIPDHHPLCEDDKLDGVGPYGQAKIQAEMVALELRAQ
                     RLVTPIIRPKSFIGPERLGVFSLLYDWALDGRGFPMLGSGKNRYQLLDVEDLCDAIWL
                     CLTLPDAVVNDTFNVGAAEFGTMGSDYQAVLDEAGFGKQVRPVPALPAVMTLRLLEKL
                     GVSPLYKWVYETASKDSFVSIDKARQKLGWRPKYSNVDALLRNFAWYKEHQAEFANAS
                     GVSHRVPWKQGAVGMMKRFF"
     gene            complement(3352..>4295)
                     /locus_tag="VHR64_11240"
     CDS             complement(3352..>4295)
                     /locus_tag="VHR64_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX3303447.1"
                     /translation="KGDADICGVRPALASGNVELQTGTYVRRLLTDKRGKKITGIETV
                     RAGAFETITAGTVVVACGAVNSAALLLRSANDTHPRGLANSSDQVGRNYMVHNNAVLI
                     AIDPRRGNPTIFQKSMAINDFYFSGPDYSYPLGNLQPVGKIQTPMLKSSVPRVPNVML
                     GPIASHSTDWWVMSEDLPDPENRVTLGTDGSIRVHWTPNNTVTHRTFIGVSRDVFREA
                     GYKVMIDRVMGIETNSHQVGTLRFGTDPASSVLDPFCKAHDLDNLYVMDASFFPSSTA
                     VNPALTIGAQAVRVADYLMVRLGGSAQHVPTSVPAGA"
BASE COUNT          774 a         1417 c         1305 g          799 t
ORIGIN      
        1 atgttgcgca gcacaaaagg aggggtgaca ggtccgcgat cagttttcgc cccgcaaccg
       61 aactgatcgc ctgctcgact tctcgcctgc tcgcctggtt attcccctgt ctgcccgtcg
      121 accagctcgc ggatcagatc ggcatgtcca caatggcggc cgacttcctc gaccatgtgc
      181 gtcaggaccc agcccacgtt gaggccgtga gccgcggctt ccgcctcctt cggcgcatcg
      241 tcccagttca cgccggccag aatctgcgcg cttcgctcgg cctcggcgtc gtatgcagct
      301 ttgatcgact cgaacgtgtc gtccggcgat ggtcgccaat cggcatcctt gtcgtcgtcg
      361 gtccagtcga actcgacggt caacccgccg atgacctgcg cgatccacca ccgctcaacg
      421 ttgaccgagt gcttgagaat gccgagcagg cacgtccccg acggaaccgg cgaccaccgg
      481 agctgctcct cagtcaatcc ggcgcacttc cagagcaatg tctcgcgcag gaagcgatgg
      541 aatcccatca ccacctcgac cggcccaccg gatcgaggcg gctcagtccg cccctgctcg
      601 tcgatgatcg gttcagtcat tggtctttcg tctctcgtct ttggtctttc gtcattggtt
      661 ttcggtgttc agttttccgc atccatcaac caaccaccat caaccctcaa cctactgctc
      721 gcctgctcgc ctgctcgcct tattcgcccg ttaccccgtc cgccagctcg cgcatcacgt
      781 ccatttggcc gaggtgacgg gcgatctccc agttgaggtg cgtcaggatc cagccgacgg
      841 tcaggccctc ttccaggctg cgctggctct tgccgatttc gtcccacttc agggtcggga
      901 tgatcgcgtc gctgcgggca gcctcttcgc gggccagcgc caccagcgac tcgaagctct
      961 cgtccggacc aaccttccag tcggcctcct cgtcctcctc ggtccacggg aagtcgacgt
     1021 cgagaccacc catggcgtag gcgatccaga gccgctcgac gtggatgcag tgcttgaaca
     1081 ggccgagcat cgtcgcgccc gaaggcaacg cgatcctacg ctgggcagca tcggtcagtc
     1141 cctcgagctt gcggatcatc gtctcccgcg agaagacgtg gaaggcgtag gtcagcgtaa
     1201 ccggatcagc ggcgcgcggc ggttgcggtc gggtcaagtc gtccaattgt caaaaacctc
     1261 acgagagata caggatgatc agtgatgtcg cgacccagcc ggcgatgcac gaaatcaggc
     1321 cgggatcctt gatcagcagc acttccggac ttccgccctc tccccgccgg tagatcaagt
     1381 agaggtagcg gaacaacccg tacgcgacga acggaatcgt gagcatcatc gcatggctgt
     1441 ccgggacatt ggcggcgtcg aacgtgtaga gcgagtaggc aatcagcatt gccgctgaaa
     1501 cgatcgcgat gatctggtca agcatcggca gactgtattc ctcgaggttg gcgcggtgat
     1561 gtcccgcggc tgcctcgagc gtgacgatct cgttccgccg tttgccgaac ccgatgaata
     1621 gcgctccgag cgcggtgcag acatagagcc acggcgacgc gggcacgctg atcgccaccg
     1681 cgccgccgac cgcgcggagc acgaaacccg ccgcgatcgc gaagacgtcg agaatcacca
     1741 tccgcttcaa accgtacgag tacgcgagca tcagcgcgat ataggccacg atcacgatcg
     1801 cgaactccgg ccgcaccgcg accgcggtaa tcagcccgac ggtcagcagt gccaccgcaa
     1861 cccgcatcgc ctgtccggga ctgacctcgc cggccgcgat tgggcggttt cgcttcttcg
     1921 gatgcagccg gtcctggtgc aggtcgcgga cgtcgttgac gaggtagatc gcgctgctga
     1981 ccgcactgaa gaccatcgcc gcagccaggg tgtgccagag cgcatcccac tggaagacgc
     2041 gcttgtcgaa cagcaacgcg gcgaagacga ggctgttttt ggaccactgc agcggccgca
     2101 tggcgcggat caacggcggc aaggcaaccc gacgcgccct taccgcttcc gcgcttcgct
     2161 ccgtgtattg cttggccggc gctgcgtcgc ccactgcttg ctccgtcaga agaatcgctt
     2221 catcatgccg accgcgccct gcttccaggg cacccggtgg gacactccac tggcattcgc
     2281 gaattcagcc tgatgttcct tgtaccacgc aaagttgcgc aagagcgcat cgacattgga
     2341 gtacttcggt cgccaaccga gtttctgccg cgccttgtcg atcgagacga acgagtcctt
     2401 ggacgccgtc tcgtagaccc acttgtagag cggcgatact cccagcttct ccagtaaccg
     2461 gagcgtcatc accgccggaa gtgccggcac cggccggacc tgcttgccga accccgcctc
     2521 atcgagcact gcctggtagt cggagcccat cgtgccgaac tcggccgcgc cgacgttgaa
     2581 ggtgtcgttg acgaccgcgt ccggaagcgt caggcagagc cagatcgcat cgcagagatc
     2641 ctcgacatcg agcaactgat accggttctt gccgctcccg agcatcggga agccgcgacc
     2701 atccagcgcc cagtcataga gaagcgagaa aacgcccagc cgctccggac cgatgaatga
     2761 cttcggccgg atgatcggtg tgaccagccg ctgcgcccgc agctcgagcg cgaccatctc
     2821 cgcctggatc ttggcctggc cgtacggacc gacgccatcc agcttgtcgt cttcgcagag
     2881 cgggtgatgg tcggggatgc cgtagaccgc cgtcgacgag atatgaacga cgcgcgcgat
     2941 gccccgttgg agggccgtcg tcatgactac acgagtcccc acaacatcgg tcgtgtagat
     3001 gtcctccggc gaatagagcg gcagtgcggc cgcgcagtgc acgaccatgt ccacatcctc
     3061 gagcgccagc tcgacggccg acttgtcgcg gatgtcgccg gtcacgatcc ggaccttgtc
     3121 tcgcacgtcc ggataatcga atggcgcgat atcgagcgac gtgacgttga cattgcgctc
     3181 gagcagatat cggaccaggt tgattcccaa aaagcccgcg ccgccggtga tcagcacgcg
     3241 tcgcggccgg attgaactat cggttgccac tcgttgctag cctgactctg atggttcgga
     3301 tgccggacat cgcgccggcc ccttgaggat acgtgttcgt acgcggttgg tctatgcgcc
     3361 ggccggtacg gaggtcggca cgtgctgggc cgagccgcca agtcggacca tcagataatc
     3421 ggccacgcgt accgcctgcg cgccgatcgt cagagccgga ttgaccgccg tcgaggacgg
     3481 gaagaacgag gcatccatga cgtagaggtt gtcaaggtcg tgcgccttgc agaacggatc
     3541 gagcaccgag ctcgccggat cggtcccgaa gcggagcgtc ccgacttgat gcgagttcgt
     3601 ctcgatgccc atcacgcggt cgatcatcac cttgtagccg gcctctcgga aaacgtctcg
     3661 cgagacgccg atgaatgtgc ggtgcgtgac cgtgttgttc ggcgtccagt gcacccggat
     3721 cgagccgtcg gtgccgagcg tgacgcgatt ctcgggatcc ggcaaatcct cgctcatgac
     3781 ccaccagtcg gtgctgtggc tcgcaatcgg accgagcatc acgttcggta cgcgcgggac
     3841 gctgctcttg agcattggcg tctgaatttt gccgaccggc tgcaggttgc ccagcgggta
     3901 cgagtaatcc gggccggaga agtagaagtc gttgatcgcc atgctcttct ggaagatcgt
     3961 cgggttgccc cggcgcgggt cgatcgcgat caggaccgcg ttgttgtgga ccatatagtt
     4021 gcggccgacc tgatccgacg agttcgctaa tcctcgcggg tgggtatcgt tggccgagcg
     4081 gagcagcagc gcggccgaat tgaccgcccc acacgcgacg accacagttc cagctgtgat
     4141 cgtctcgaag gcgccagccc ggacggtctc gatgcccgtg atcttcttgc cgcgcttgtc
     4201 ggtgagcagg cggcggacat aggtgccggt ctgcagttcg acattgccgg acgccagcgc
     4261 gggacggaca ccgcagatat cagcgtcgcc cttcg
//
LOCUS       DATAOZ010000439         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2543926, whole genome shotgun sequence.
ACCESSION   DATAOZ010000439 DATAOZ010000000
VERSION     DATAOZ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543926"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VHR64_11245"
     CDS             complement(<1..670)
                     /locus_tag="VHR64_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303448.1"
                     /translation="MSGYELRLGRLFNGNTGRSFITAFDHAVTLGVPEGGEDAFGTLG
                     KIIEGGPDAVLVSPGMLNKAGHMFADRNAPAPIVRCDFIINHPYLNDLGEGYRKICSA
                     EQAARLGADAVVMFLMVGAGHCELFKENVTALTEMAEAAHNVGLPLIVESVLWGTRIE
                     NKKDPEMLAFGARMAVELGADAIKTEYTGDAASMKDIIESCPAPVLLLGGTKSSDPEA
                     LWQGT"
     gene            complement(836..1240)
                     /locus_tag="VHR64_11250"
     CDS             complement(836..1240)
                     /locus_tag="VHR64_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303449.1"
                     /translation="MPDTLIAVVPRSRLSAILTAFHRNGYGHVTRVLDPDRAPVAQQL
                     GRAGVHESRIASADLADAVVLFVPAPKRTTDAAALAVAHGATDAEVVTGGTPVSAAVV
                     PGLITFAGHRRGRRAERRTPRAAEIGPDLTAE"
     gene            complement(1316..>1992)
                     /locus_tag="VHR64_11255"
     CDS             complement(1316..>1992)
                     /locus_tag="VHR64_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX3303450.1"
                     /translation="FLAIPVVDDENRLVGIVTAEDVADVLEEEATEDIERLGGSAPLS
                     EPYLRASPALLFRKRIVWLLVLFMAQFVTLEVYDHYQGVLQEQLVLAMFVPILIGTGG
                     NVGSQTVTTIIRAMAVGEAAPRHFVRILGKEIVTALGLGAVMGMLMFARGAITNGREF
                     GFTVGLTVGVLIIWAATVGAMLPLVLSKLRVDPAVVSAPFISSLVDATGLIIYFTVAG
                     LILHVG"
BASE COUNT          375 a          667 c          625 g          325 t
ORIGIN      
        1 tggtgccttg ccagagcgcc tcgggatcgc tgctcttggt cccgcccagc agcaggaccg
       61 gcgccgggca gctttcgata atgtccttca tgctcgccgc gtcaccggtg tactcggtct
      121 tgattgcgtc cgcgccaagc tcgacggcca tgcgcgcgcc gaacgccagc atctccggat
      181 ctttcttgtt ctcgatccgg gtgccccaga ggaccgattc gacgatcagc ggcagaccga
      241 cgttgtgcgc ggcctcggcc atctcggtga gggccgtgac attctccttg aacagctcgc
      301 agtgcccggc gccgaccatc aggaacatga cgacggcatc tgcgccaagg cgagcggcct
      361 gctcggccga gcagatcttg cgatacccct cgccgagatc gttgaggtac gggtgattga
      421 tgatgaagtc gcagcggacg atcggcgccg gcgcgttgcg gtcggcaaac atgtggccgg
      481 ccttgttgag catgccggga ctaaccagca ctgcatctgg tccgccctcg atgatcttgc
      541 ccaaagtccc gaaggcatcc tcgccacctt ccggcacgcc gagcgtgacc gcgtgatcga
      601 aagccgtaat gaacgaccga cctgtgttgc cattgaaaag ccgcccgagg cgcagctcgt
      661 acccgctcaa cgaaactccc ttgctcttga taacaccggc ggacgggccc acccgcccac
      721 tcagcaattc ctgagtcgtg aaacttccgt aatgctatgg gccagtccga atcccgtcaa
      781 caggtctgtg cagacgcacc aggatagact cgcgcagctt cggcgtgcgt cggtgctatt
      841 cggcggtgag gtccggaccg atctcagctg cacgcggggt ccgacgttca gcgcggcgcc
      901 cccgacgatg cccggcgaag gtgatcaggc ccggcacaac cgccgctgaa acgggcgtgc
      961 cgccagtcac tacctcggca tcggtcgccc catgcgcaac ggcaagcgcg gccgcgtcgg
     1021 tggtccgttt cggcgccggg acgaacagga ccacggcatc tgcaaggtcc gcactggcga
     1081 ttcggctctc gtgcacgccg gcccggccga gttgctgcgc gaccggcgcg cggtccggat
     1141 cgagtacccg cgtgacatgc ccgtagccgt tgcgatggaa cgcggtcagt atcgcgctga
     1201 ggcgagagcg tggaacgacg gcaatcaggg tgtcgggcat gagcgggttc ctggatcaga
     1261 tcgtgactcg aagtcaacaa cgtccgatca gaataccgcg agcgtacgaa caggtctacc
     1321 cgacgtggag aatcagaccg gccacggtga agtagatgat caggccggtg gcatcgacca
     1381 ggctgctgat gaacggcgcc gagacgactg ccgggtccac gcgcagcttg ctcaggacaa
     1441 gcggcagcat ggcgccgacc gtcgcggccc agatgatcag cacgccgacg gtcaatccga
     1501 ccgtgaagcc gaactcgcgc ccgttcgtga tcgcgccgcg ggcgaacatc agcatcccca
     1561 tgaccgcgcc caggccaagg gctgtgacga tctccttgcc cagaatccgt acgaagtggc
     1621 gcggggccgc ctcgccgacc gccattgcac ggatgattgt ggtcaccgtc tgcgagccga
     1681 catttccgcc ggtcccgatc aggatcggca cgaacatcgc caaaacgagc tgctcctgga
     1741 gcacgccctg gtaatggtcg tagacctcga gcgtcacgaa ctgggccatg aagaggacca
     1801 gaagccagac gatccgcttc cgaaaaagca gcgccgggga tgcgcgcagg tacggctcgg
     1861 acagcggtgc ggaaccgccg agccgctcga tgtcctcggt cgcctcctcc tcgagcacgt
     1921 ccgcgacatc ctccgcggtc acgatcccga ccagccgatt ctcgtcatcc acgaccggaa
     1981 tagccaggaa at
//
LOCUS       DATAOZ010000440         7275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5173176, whole genome shotgun sequence.
ACCESSION   DATAOZ010000440 DATAOZ010000000
VERSION     DATAOZ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5173176"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1633
                     /gene="glmS"
                     /locus_tag="VHR64_11260"
     CDS             <1..1633
                     /gene="glmS"
                     /locus_tag="VHR64_11260"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX3303451.1"
                     /translation="KQPGKIATATVDMPPADIGFGHTRWATHGGVTQANAHPHLGESG
                     RLAVIHNGIVENFRDLRNGLSARGHQFKSETDSEVIAHLLEERLTGGEEDTLRAFRAA
                     FSELDGLSAVIALDLGSRSLIAAKSVSPLVVGRGPDGVTIASDALALHGHADEVMYLE
                     DWHLVQLTKHGIDVFNRETLELVDGEWVPLQLEERDVSLGSHPHFMSKEMHEQPDVLE
                     RLVREGEEDIKALATAIDESFGTFLVGCGTASYAALSGAYLFSRIAAKHVNFAVGSEF
                     KYHEHFLTPQSLVVALSQSGETADVIESMLAARTRGARLGALVNVPRSTLDRLVDVRV
                     PLRAGPEQCVLSTKAYSAKVAALLLTAHALNGTWDEGRDLVLRAAAGLREMLTDPWID
                     RVREVARYVYQHDHLFVIGRGLAYPTALEAALKIKEVSYIHAEGFAGGELKHGVIALV
                     APGTPCVVYAPSDETRADIISGAMELKSRDGYIIGVSSMSDPVFDVHIPVPEVGDASP
                     LVNALPAQMLGYHAALLRGNDPDKPRNLAKSVTVK"
     gene            1630..2424
                     /locus_tag="VHR64_11265"
     CDS             1630..2424
                     /locus_tag="VHR64_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX3303452.1"
                     /translation="MITHRYIDTHTHLDDKQYINDLDAVLTDAMAAGVSGMVNIGYCP
                     ERWTTTLALAERYPRVSYALGLHPGNADEWSESLMDDLTSLLERVDPVAVGEIGLDYY
                     WTQENKAVQRMSFERQLELAHSRSLPIIIHQRDAAADVAAILRNAPADLRVVLHSFDG
                     DPELSDLARERGWMIGVGGLMTRRQSEALRSGLPGFPLEQMLLETDSPYLVPSGVKAR
                     RNVPAMIPLIAERLAGVINRTVDDIAQLTTRNAERIFGLKATATAP"
     gene            2421..3041
                     /gene="rdgB"
                     /locus_tag="VHR64_11270"
     CDS             2421..3041
                     /gene="rdgB"
                     /locus_tag="VHR64_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX3303453.1"
                     /translation="MTDLLLASDNPGKLAELQSLLPPDVIVRSLSDVGLEPPEETGAT
                     IAENAALKAITAARASGLLTLADDSGLEVEALGGRPGVRSARFAGEEATDTQNNELML
                     SEMSAVPENHRAAQFVCTLTLANPDGVLASATGRCRGSIGYESRGSNGFGYDPLFVLQ
                     DGRTMAELGSAEKNQISHRGKALREILPAVLIAIGAHRFHAQGAGQ"
     gene            3038..4276
                     /locus_tag="VHR64_11275"
     CDS             3038..4276
                     /locus_tag="VHR64_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303454.1"
                     /translation="MTTQLSTCAVRSSFGEWAFEAGQNGLHPMIYVVGTRGKSTVARL
                     LDTIARESGLRTALRTDSGVEIEGKRQMGDIHPLVEALECLDSGELDLAIIEMDWSDL
                     HTLPVGDRQPGAVIVSTICPHKEYCLLEDTKRAIAGLKALLLSTPESTMIAADIDDAA
                     FPSLNDVNFENMILTTVSETHPSLRRFLAQGGLAAWTSDHDLVVGSRDSVNLRMPIDE
                     IPVTLDGAVLFQMRNVMLASSVAAAIGIPAESVRAGLLRVQPDHANLPTSLNSFVTDG
                     GIRVLIDRPSPSWFLGPLLRAARGMKPKRTIYVVDYRDMPNHDDSVEVGRMIGRNASM
                     CVMINDEKSLASVMAVKAGMAQNDVPPPIAHVETLPKAIQRALSAAREDDLIVVLTNQ
                     VQTVYRSMARHRVADSSPAK"
     gene            complement(4292..>7275)
                     /gene="dnaE"
                     /locus_tag="VHR64_11280"
     CDS             complement(4292..>7275)
                     /gene="dnaE"
                     /locus_tag="VHR64_11280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX3303455.1"
                     /translation="LAELFGPERFYLEIQDHGIKEQKLANDHLIPMARELGVPLVATN
                     DVHYVSPEDSPIQDLVVCVQTNTTLSDPKRLKSESDQLYFKSPQEMWDIFKDVPEALS
                     NTIRIAEMCDVDLSFRGYQLPDFDVPEGFTPDSYLEHLVREGAKRLYGHDSGEVGDRL
                     AFELDVIRSMGFTNYFLVVWDFIRYAREQDILVGPGRGSAAGSIVTYSLGITALDPLK
                     HGLIFERFLNPSRISMPDIDIDFADTGRDKVIKYVVDKYGDDRVAQIVTFGTLAAKAS
                     VRDIGKAMELPFPDIDRIAKLIPTGPGVTIDKAMERVPELKALYDSDKQARDVIDAAR
                     RVEGLARHASTHAAGVVISRDPLVEHVPLQRAGGKSEGDITTQYQMGLLEDIGLLKMD
                     FLGLSTLTILGKAVENAHAKDPNLTVDTIPLEDEKAFELLRNGDTVGIFQLEGGMTTK
                     MTTDIQPRRFEDVVALMALIRPGPMTMAPEFFARKNGETECIYIHPDLEPILAETYGI
                     TLYQEQVIQIANRLAGFSMAEGDGLRKAMGKKLPEEMAKMRQRFVDGCVQNKIEKSIG
                     VELFDMIERFAGYGFNKSHSAAYAVIAAQTAYMKAHYPVEFMAAVLSVDIGSTEKVVY
                     DVSECRRAGIRVLPPDINRSVRDFSVEQTEDGREAVRFGLGAVRNVGEGAIQAILEAR
                     ADQPGETFASLDALCSAVDWNAVSRRVFESLAKGGALDCFGERGAVLANLDRAIAAGQ
                     TRHKAKSRGQIGLFGAEEAASASVATLTLDGPPLGQRELLAFEKEAIGLYLSSHPLAN
                     VIRGKLPEGYAEIIRLPEMPAAQPVRVIASIRSIRRIATRQNKTMAAIEIEDLTDRIE
                     AVLFPAAYEEFGTELEADQIIEITGKVDRRNDQLQIIVDKVTTDVAPIEPELPSRRIL
                     LRLPQSEDYWQDVEVLHKLDAILNDHGGPDRIEFELTVGGRPVRIVNRKHRVEWDDEL
                     ASELRAALGADGVQVSEPIAS"
BASE COUNT         1327 a         2305 c         2285 g         1358 t
ORIGIN      
        1 gaaacagccc ggcaagatcg ccaccgcgac ggtcgatatg ccccccgccg atatcggttt
       61 cggccacact cgctgggcga cgcatggtgg cgtcacccag gccaacgcgc acccacacct
      121 tggcgaatcc ggccggctgg cggtgatcca caatggcatc gtcgagaact tccgggattt
      181 gaggaatggc ctctcagcac ggggtcacca gttcaaatcg gaaaccgact cggaggtcat
      241 cgctcacctc cttgaagaac ggctcaccgg tggcgaggaa gacacgctcc gagccttccg
      301 tgcggcattc agcgaactgg acggcctgag cgccgtgatc gcgctcgacc tcggcagccg
      361 gtcgctgatc gcggccaagt cggtctctcc cctcgttgtc ggtcggggac cggacggggt
      421 cacgatcgcc tcggacgctc tggccttgca tggtcacgcc gatgaggtga tgtatctcga
      481 ggactggcac ctggttcaac tgacgaagca tggcatcgac gttttcaacc gggagacgtt
      541 ggaactcgtc gacggcgaat gggtcccgct ccaactcgag gagcgcgacg tttcgctcgg
      601 ctcgcacccg cacttcatgt ccaaggagat gcatgagcag ccggatgtcc tggagcggtt
      661 ggtgcgcgag ggcgaggagg acatcaaggc gcttgccacg gcgatcgacg aatcgttcgg
      721 cacgttcctg gtcggctgcg ggaccgcgtc gtatgcggca ctgagcggcg cctacctgtt
      781 cagccgaata gcagccaaac acgtcaactt cgcggtcggc tcagagttca aatatcacga
      841 gcatttcctg acaccgcaaa gcctggtcgt ggcgctctcg cagtccggcg agacggccga
      901 cgtgatcgag tcgatgctgg cagcccggac gcgcggcgcg cggctcgggg cgctggtcaa
      961 tgttccacgc tcgacgctgg accgcctggt cgacgtgcgc gtgccgctcc gggccggacc
     1021 agagcagtgc gtcctctcga ccaaggccta ctcggccaag gtcgcggcgc tgctgctgac
     1081 cgcgcacgcg ctgaacggaa cgtgggatga gggtcgggac ctggtcctgc gtgctgccgc
     1141 tggcctgcgt gaaatgctga ccgatccgtg gatcgaccga gttcgggagg tcgcgcggta
     1201 cgtctaccag cacgatcacc ttttcgtgat cggtcgcggt cttgcctacc cgaccgcgct
     1261 cgaggccgcg ctgaagatca aagaagtcag ctatatccat gccgaggggt ttgccggtgg
     1321 cgagctcaag catggcgtca tcgccctcgt cgcgcccggc accccgtgtg ttgtctatgc
     1381 gccgagcgat gagacgcgag ccgatattat ttcaggagcg atggaactca agagccgcga
     1441 tgggtacatt atcggggtca gctccatgag cgaccctgtc ttcgatgttc acatccccgt
     1501 tcccgaggtg ggcgacgcct cgccgctggt gaatgcgctg cctgcccaaa tgcttggata
     1561 tcatgcggcc ctgctccggg ggaacgatcc ggacaagccg cgcaaccttg caaagagcgt
     1621 cacagtgaag tgatcacgca cagatatatt gacacgcaca cgcatctcga cgataagcaa
     1681 tacatcaacg atctcgatgc agttctgacg gacgcgatgg cagccggagt ctcaggcatg
     1741 gtcaacatcg gctactgccc ggagcgatgg acaaccaccc ttgcgcttgc cgagcgatat
     1801 ccccgggtga gttatgcgct cgggctgcat cccggcaacg cggatgagtg gtcggaatcg
     1861 ctgatggatg acctgacgtc ccttctcgaa agggtggatc cggtcgcggt cggtgaaatc
     1921 ggtctcgatt actactggac gcaggagaac aaagcggtcc agcgaatgag tttcgagcgt
     1981 cagctcgagt tggcgcattc gcggtccttg ccgatcatca tccaccagcg agatgccgct
     2041 gcagatgtcg ctgcgatcct gcgtaacgcg ccggcagatc tccgggtcgt gctccacagc
     2101 ttcgatggcg accctgagct gtccgacctg gccagggagc gtggctggat gatcggcgtc
     2161 ggcgggctga tgacccggcg ccagtccgaa gcgctccgat ccggcctgcc cgggtttccg
     2221 ctcgagcaaa tgctgctcga gaccgattcg ccctatctcg tgccgtccgg agtcaaggca
     2281 cgccgcaacg tgcccgcgat gattccactg atcgccgagc ggctcgctgg agtgattaac
     2341 cgaacggtcg atgacattgc ccagctcacg acgcgcaatg cggagcgaat cttcgggttg
     2401 aaggcgacgg caaccgcgcc atgaccgact tgcttctggc cagcgacaac ccgggaaaac
     2461 tcgctgagct tcagtcgctc ctgccgccgg atgtgatcgt ccggtcactc agtgatgtcg
     2521 gcctggagcc gcccgaagag actggggcca cgatcgccga gaacgccgcg ttgaaggcga
     2581 ttacggcggc gcgggcgagc ggattgttga ccctggcgga cgattccggc ctcgaggttg
     2641 aggcgctggg cggacggccc ggagttcgat cggcccgttt cgcgggcgag gaggcgactg
     2701 atacgcaaaa caatgagctg atgctgagtg agatgtcggc ggttccggaa aaccatcggg
     2761 ccgcgcaatt cgtttgcaca ttgacactcg caaatccgga cggcgtcctc gcaagcgcca
     2821 ccggccggtg caggggctcg atcgggtacg agtcgcgcgg ctccaacgga ttcgggtacg
     2881 acccgctctt cgtcctgcag gacggacgca ctatggcgga gcttggctca gccgagaaga
     2941 atcaaatcag tcaccgtgga aaggcgctgc gggagatctt gccagcagta ttgatcgcca
     3001 tcggcgccca tcgatttcat gcacaaggag caggtcaatg acgacccagt tgtccacgtg
     3061 cgccgttcgt agctcgttcg gtgagtgggc atttgaagcg gggcagaacg gactgcaccc
     3121 gatgatctac gttgttggta ctcgtggcaa aagcaccgtc gcccgacttc tcgacaccat
     3181 cgcccgtgaa tccggcctcc ggacggcact tcgcaccgac agcggtgtgg agatcgaggg
     3241 caagcgccag atgggcgata ttcatcccct ggtcgaggcg ctcgaatgcc tcgattccgg
     3301 cgagcttgat ctcgcgatca tcgagatgga ctggagcgac ctgcacacgc tgcccgtggg
     3361 cgatcgccag ccgggcgcgg tcatcgtctc gaccatctgc ccgcacaagg aatactgtct
     3421 gctcgaggat acgaagcgcg cgatcgccgg actgaaggcg ctgctgcttt cgacgccgga
     3481 atcgaccatg atcgccgcgg acatcgacga tgcggcattc ccgtcgttga acgacgtcaa
     3541 cttcgaaaac atgatcctga cgactgtcag tgaaacgcat ccgtcgctcc gacgctttct
     3601 cgcccagggc ggattggctg cctggacgag tgatcacgac ctggtcgtcg gttcgcggga
     3661 cagtgtcaac ctgcgcatgc cgatcgatga gatcccggtc acgctcgatg gcgccgtcct
     3721 cttccagatg cgcaacgtca tgcttgcctc ttccgtcgcc gcggcaatcg gcatcccggc
     3781 agaatcggtg cgagccggac tgctgcgcgt ccaacccgat cacgcaaacc tgccgaccag
     3841 cctgaactcg ttcgtgacgg atggcggcat ccgcgtgctg atcgaccggc cgagcccatc
     3901 ctggttcctc ggtccgctac tgcgagccgc ccgcggaatg aagccgaagc ggacgatcta
     3961 cgtagtcgat taccgggata tgcccaatca cgacgattcg gtggaagtcg gacggatgat
     4021 cgggcgcaac gcgagcatgt gcgtcatgat caacgacgag aagtcgctgg ccagcgtcat
     4081 ggcagtcaag gccggcatgg cgcagaatga tgtgccaccg ccgatcgcgc atgtcgagac
     4141 gttgcccaag gcgattcagc gagcactctc cgccgcgcgc gaggacgatc tgatcgtggt
     4201 gctgaccaat caggttcaga cggtctaccg ctcgatggcg cgtcacaggg tcgctgactc
     4261 gtcgccggca aagtaacgca cccgccgcgg gtcagctggc gatcggctcg ctgacctgca
     4321 ccccatctgc cccaagcgcg gcgcgaagct cggaagcgag ctcgtcgtcc cactcaacac
     4381 ggtgtttgcg gttcacgatg cgcaccggcc gaccaccgac ggtcagctcg aattcgatgc
     4441 ggtccgggcc gccgtgatcg ttcaggatcg cgtcgagctt gtggagaacc tccacatcct
     4501 gccaataatc ctcagactgg ggcaggcgaa gcaagatccg gcgtgatggc agctcgggct
     4561 cgatcggagc gacgtccgtc gtgactttgt cgacgatgat ctggagctga tcgttccggc
     4621 gatcgacctt gccagtgatc tcgatgatct ggtcggcttc cagctcggtg ccgaactcct
     4681 cgtaggcggc cgggaagagc accgcttcga tccgatcggt caggtcctcg atctcgatcg
     4741 cggccatcgt cttgttctgc cgtgtcgcaa tcctgcggat gctccggatc gacgcgatca
     4801 cgcgcaccgg ttgcgccgcc ggcatctccg gcagtcggat gatctccgcg tatccctcgg
     4861 gcaacttgcc cctgatcacg ttggccagcg ggtgggaact caggtacagg ccgatcgctt
     4921 ccttttcgaa cgcgagtagc tcgcgctgcc ccagcggcgg gccatccagc gtcagggtcg
     4981 caacgctcgc gctggccgcc tcctctgctc cgaacaggcc gatctgaccg cggctcttcg
     5041 ctttatgtcg cgtttgcccg gcggcgatgg cacggtcgag gttggccagc accgccccac
     5101 gctccccgaa gcaatcgagc gcgccgccct tcgccaggct ctcgaacacg cgccgcgaaa
     5161 ccgcgttcca atctaccgcg gagcacaacg cgtccaggct ggcgaacgtc tcgccgggct
     5221 ggtcggcccg ggcctccaga attgcctgga tcgcgccttc gccgacattg cgaactgcac
     5281 cgagaccgaa ccgaaccgcc tcgcgcccgt cctcagtctg ctcaaccgaa aagtcgcgaa
     5341 cgctccggtt gatatccggc ggcaggaccc tgatcccggc ccgacggcac tcagacacgt
     5401 cgtagacgac cttctcggtc gagccgatat cgactgagag gaccgcggcc atgaactcga
     5461 ccgggtaatg ggccttcatg taggcagtct gcgcggcgat taccgcgtag gccgcgctgt
     5521 gggacttgtt gaagccgtac ccggcgaatc gctcgatcat gtcgaacaac tcgactccaa
     5581 tcgacttctc gatcttgttc tgaacgcagc catccacgaa gcgctggcgc atcttggcca
     5641 tctcttcggg cagtttcttg cccatagcct tgcgcagccc gtcgccttct gccatcgaga
     5701 agccggccag ccggttcgcg atctggatca cctgctcctg gtaaagcgtg atgccatagg
     5761 tttcggccag aatcggctca aggtcggggt ggatgtagat gcactcggtc tcgccgttct
     5821 tgcgggcgaa gaactcgggc gccatcgtca tcggtcccgg ccggatcagc gccatcagcg
     5881 cgacgacgtc ctcaaaccgg cgcggctgga tgtcggtcgt catctttgtg gtcatgccac
     5941 cttccagttg gaagatgccg acggtgtcgc cgttccgtag cagctcgaag gccttctcgt
     6001 cctcgagggg aatcgtgtcc acggtcagat tcgggtcctt ggcgtgcgcg ttctcgaccg
     6061 ccttgcccag gatggtcagc gtggacaggc cgaggaagtc catcttgagc aggccgatgt
     6121 cctcgagcag acccatctgg tattgcgtcg tgatatcgcc ttcgctcttg ccgccggcgc
     6181 gctggagcgg cacgtgctcg accaggggat cgcgcgagat gacgacgccg gcagcgtggg
     6241 tcgacgcgtg cctggccaga ccctcgacgc gccgggcggc atcgatcacg tcccgcgcct
     6301 gtttgtccga gtcgtaaagc gccttgagct caggcacgcg ctccatcgcc ttgtcgatgg
     6361 tgacgcccgg acctgtcggg atcagcttgg cgatccggtc gatatccggg aacggcagct
     6421 ccatggcctt gccgatatcc cgcaccgacg ccttggctgc gagcgtcccg aacgtaacga
     6481 tctgggcgac acggtcgtcc ccgtacttgt cgacgacgta tttgatgacc ttgtcgcggc
     6541 cggtatcggc gaagtcgata tcgatatccg gcatcgagat gcgcgacggg ttgaggaaac
     6601 gctcgaagat gagaccgtgc ttgagcggat cgagcgcggt gatgccgagg gagtacgtga
     6661 cgatgctgcc ggcagccgag ccccggccgg gtccgaccag gatgtcttgc tcacgcgcgt
     6721 accggatgaa gtcccagacg accaggaagt aattggtaaa gcccatcgac cggataacgt
     6781 caagctcgaa tgccagtcga tcgccgacct caccggaatc gtgtccgtag agccgctttg
     6841 ctccctcacg gacgagatgt tccaggtagc tgtccggcgt gaatccctcc ggcacatcga
     6901 aatcgggaag ctgataaccg cggaaactga ggtcgacgtc acacatctcg gcgatgcgaa
     6961 tcgtgttgga aagcgcttcc gggacatcct tgaagatgtc ccacatctct tgcggactct
     7021 tgaaatagag ctggtccgat tcgctcttga gccgcttcgg atccgagagc gtcgtgttgg
     7081 tctggacgca gacgaccaga tcctggatcg gcgaatcctc cggcgagacg taatgcacgt
     7141 cgttcgtcgc gacgagcggc acgcccaact ctcgcgccat cgggatcagg tgatcgttgg
     7201 caagcttctg ctccttgata ccgtgatcct ggatctcgag gtagaagcgc tccggcccaa
     7261 agagctcagc cagcg
//
LOCUS       DATAOZ010000441         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4384847, whole genome shotgun sequence.
ACCESSION   DATAOZ010000441 DATAOZ010000000
VERSION     DATAOZ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4384847"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VHR64_11285"
     CDS             <1..272
                     /locus_tag="VHR64_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303456.1"
                     /translation="LDAITCEMTVPQVGLIDGDAISLSVAAASILAKTERDRFMRQMH
                     EQDARYQFDRHVGYGTPIHLQALRIHGPCSLHRITFRGVLPEDGA"
     gene            269..643
                     /locus_tag="VHR64_11290"
     CDS             269..643
                     /locus_tag="VHR64_11290"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX3303457.1"
                     /translation="MTTERTKLGSVGEHHARLILERKGMAFVEANWRAPSGEIDLVMR
                     DGELLVMVEVKVRRGERAGTAEESISRAKATRLLVTGEWYVAEHPEHADRPWRIDLVA
                     ITLDGRGVVTRVQHIEDAVVAG"
     gene            717..>1967
                     /gene="hisS"
                     /locus_tag="VHR64_11295"
     CDS             717..>1967
                     /gene="hisS"
                     /locus_tag="VHR64_11295"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX3303458.1"
                     /translation="MTDTQSTGRRVKAQVLSGFRDQLPAQMLLRRTLIDRVRKVFEAH
                     GFEPIDTPTLEHLEVLTGKAGENEKLMYHFLDHGDRAVGLRYDLTVPLARFVANHQNE
                     LPMPFKRYHIAPVWRAEKAQRGRFREFWQCDADIVGSASARADAEAISMVAEILAAVG
                     LPQFTVNISHRKLLEGLARGAGVPSELAPTVYRAVDKLNRIGAGGVRSELVDAGITGE
                     AADRLLALISQTGDNRALIESLRESVGDDAEVMQALENLAELFTMLEDLAPGGRCFRF
                     DLTLARGIDYYTGIVYEAMVDEPKVGSVSGGGRYDGLVGMFSGRDIPAMGISIGLERI
                     IEVAEEFNLVSAPSSVCDAIVIYHDDTFSYASDVARKLRADEFNIDLSLLGRKSFGDQ
                     LKYADRRGIPLAVLIGPDEAAAGTA"
BASE COUNT          341 a          617 c          656 g          353 t
ORIGIN      
        1 tcctcgacgc cattacttgc gagatgaccg tcccgcaggt cggtttgatc gacggcgacg
       61 caatttcgct gtcggtcgcc gccgcgtcga tcctggccaa gaccgagcgg gaccggttca
      121 tgcggcaaat gcatgagcag gatgcgcgat accagttcga ccgccacgtc ggctacggca
      181 caccgatcca cttgcaggcg ctgcgcatcc atggtccgtg ctcgctgcac cgcataacct
      241 ttcggggcgt cttgccggaa gacggcgcat gacgaccgag cggacgaaac tcggctcggt
      301 cggcgagcat cacgcccggc tgatccttga gcggaagggc atggccttcg tagaagcgaa
      361 ctggcgcgct ccctccggcg agatcgacct cgtcatgcgc gacggcgagc tcctggtcat
      421 ggtcgaggtc aaggtgcggc ggggcgagcg cgcaggcacg gccgaggagt cgatttcccg
      481 ggcgaaagcg accaggctgc tggtgaccgg cgagtggtat gtcgccgagc acccggagca
      541 tgccgaccgg ccatggcgaa tcgatctcgt cgccatcacg ctcgatgggc gcggagttgt
      601 tacccgtgtg cagcacatcg aggacgcagt cgtcgccggg tgagcccaac ctcccgtaaa
      661 atcagtcgat gcaagcgcga ttttgcgcgt ccctccctgc aggagaaacc gattcaatga
      721 cagataccca gtcaaccggg cgccgcgtga aggcgcaggt cctctcaggt tttcgcgatc
      781 aactcccggc ccagatgctg ctccggcgga cgttgatcga ccgtgtccgg aaggtctttg
      841 aggcgcacgg cttcgagccg atcgatacgc cgacgctcga gcacctggaa gttctgactg
      901 gtaaggccgg cgagaacgag aagctgatgt accacttcct cgatcacggc gatcgcgcgg
      961 ttggcttgcg ctacgacctg accgttccgc tcgcgcgatt tgtcgctaat caccagaacg
     1021 agctgccgat gccgttcaag cggtatcaca ttgcgccggt ctggcgggcc gagaaggcgc
     1081 agcggggccg gttccgcgag ttctggcagt gcgacgccga tatcgttggc tccgcctcgg
     1141 cgcgagccga tgccgaggcg atctcgatgg ttgccgagat cctggcggcc gtcggtctgc
     1201 cccagttcac ggtcaacatc agccaccgca agctgctcga gggcctggct cgcggcgcgg
     1261 gcgtgccgtc cgagctggcg cctacggtct accgggctgt cgacaagctc aacaggatcg
     1321 gtgcgggcgg cgtgcggtcc gagctcgtcg acgcggggat cacgggtgaa gcggccgacc
     1381 gcctgctggc gctgatcagc cagacagggg ataaccgggc tctgatcgag tcgctgcgag
     1441 aaagcgtcgg ggatgatgcg gaggtcatgc aggcgcttga gaacctggcg gagctcttca
     1501 cgatgctcga agacctggca cccggcgggc gctgtttccg gttcgacctg acacttgctc
     1561 gcggcatcga ctactacact ggcatcgtct acgaggcgat ggtcgacgag ccgaaggtcg
     1621 gatccgtctc cggtggcggg cgatacgacg ggctggtcgg catgttctcc ggtcgcgaca
     1681 tcccggctat gggcatctcg attggtcttg agcgcattat tgaggtcgcc gaggagttca
     1741 acctggtttc agcaccctcg tcggtctgtg acgcgatcgt gatctaccac gacgacacgt
     1801 tcagctacgc gagcgacgtt gcccgcaagc tgcgcgcgga cgaattcaac atcgacctgt
     1861 cactactcgg gcgcaagagc ttcggcgatc agctcaagta cgccgatcgc cgcggaatcc
     1921 cgttggccgt cctgattggt cctgacgagg cggcggccgg cacggct
//
LOCUS       DATAOZ010000442         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7283425, whole genome shotgun sequence.
ACCESSION   DATAOZ010000442 DATAOZ010000000
VERSION     DATAOZ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7283425"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VHR64_11300"
     CDS             complement(<1..405)
                     /locus_tag="VHR64_11300"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3303459.1"
                     /translation="MPTIQLRQLVAGMAPMLLLYILYTVIRFLVKDRGPVEGIRNGYE
                     VLHLEQRLGLDWELSLQEVMLPHEWLVKAANWYYVIGFLPVILACASIAAILDFRTFL
                     WWRKRFALTMILALIGFGLFPLAPPRMLPGMVD"
     gene            complement(610..912)
                     /locus_tag="VHR64_11305"
     CDS             complement(610..912)
                     /locus_tag="VHR64_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303460.1"
                     /translation="MKDALTTNEPPDVPREPGYVLHVSDRTTAAMQLAAHFTAQSMVF
                     SSTQDPRARLAEQLSYFDLAFRAIMSSTARTLIKTPAPGSEADVDGRRVTNEFRVG"
     gene            complement(996..>1733)
                     /locus_tag="VHR64_11310"
     CDS             complement(996..>1733)
                     /locus_tag="VHR64_11310"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX3303461.1"
                     /translation="GVPYGGVDTERLISSLEIHEVDAERRVTVGEKGISPTHSLINAR
                     QEMFDNVYWHHTNRACMAMLLRAVQEGLIAGKIAADDLTGLDDAGLLSLLSDPRMPEP
                     TRRLCQALIERRIHKRAIEVSARAGQLYRQLGALYADPARRRSVEHRLDDAIATRIGS
                     PVPPGAILIDIPKPEKWRTDVLVSFRRPPIGFERVMAWREVAGLADADLKRYEDHRRL
                     IRIVTTADLGEIVREQWESILPAALGD"
BASE COUNT          315 a          544 c          564 g          310 t
ORIGIN      
        1 gtcgaccatt cccggcagca tcctcggcgg agccagtggg aagagcccga acccgatcag
       61 ggccaggatc atcgtcagag cgaagcgttt gcgccaccag aggaaggtcc ggaaatcgag
      121 gatcgccgct atcgacgcgc aggccaggat cacgggcaga aacccgatca cgtagtacca
      181 gttcgctgcc ttcaccagcc actcgtgcgg cagcatcacc tcttgcaggc tgagctccca
      241 atccagtcca aggcgctgct cgagatggag cacctcgtac ccattgcgga tgccctcgac
      301 tggcccgcga tccttgacca ggaaccggat gaccgtgtac aggatgtaga ggagcagcat
      361 gggtgccatc ccggcaacca gctgtcgcag ctggattgtc ggcaccgatc tggttcgttc
      421 tgaatagagc gcggacgacg atgacacgac gctccctccg caagagccga taccgtttgc
      481 gaccattggg cagtgcgccg gggatccgtg cggctcttat ctgcctattg gttgcaatag
      541 tagaacgaaa cggcagagcg gcaaagccat gtcggcgtga cgactcggtt ggcggggtct
      601 ggccggagtt tacccgacgc gaaactcgtt cgtcacgcgg cggccgtcga cgtcggcctc
      661 ggagcctggg gccggggttt tgatcagtgt tcgcgcggtg ctgctcatga tcgcacggaa
      721 ggccagatcg aagtagctga gttgctcggc caggcgcgcc cggggatcct gggtggatga
      781 aaacaccatg gattgcgcgg tgaagtgggc ggcaagctgc atcgccgcgg tggtgcgatc
      841 actgacatgt agcacatacc cgggttcgcg cggcacatcc gggggttcgt tggtagtcag
      901 cgcatctttc atggggccag catacctctc catttcccct gatctcaccg gtaaatctgg
      961 cccattcgta ggggtattca tttctcgatt tcgggtcaat cgccgagcgc cgccggcaag
     1021 atcgattccc actgctcgcg gacgatctcc cccagatcgg cggtcgtcac gatccggatc
     1081 aggcggcggt gatcttcgta ccgcttgagg tccgcatcgg ccagaccggc gacctcccgc
     1141 caggccatga cccgctcgaa gccgatcggt ggccggcgaa atgagaccag cacatcggtc
     1201 cgccatttct ccggcttggg gatatcgatc aggatcgcgc cgggcggcac cggagaccca
     1261 atgcgtgtcg cgatcgcgtc atcgagccga tgctctaccg aacgccgccg ggcggggtcg
     1321 gcatagagcg cgccaagctg gcggtagagc tgaccggccc gggccgagac ctcgatcgcc
     1381 cgcttgtgaa tgcgccgctc gatcaaggcc tggcagaggc ggcgcgtcgg ctccggcatg
     1441 cgcgggtccg aaaggagcga gagcagaccg gcatcgtcga ggccggtcag gtcgtcggcc
     1501 gcgatcttgc cggcgatcag cccttcctgg accgcgcgca acagcatcgc catgcaggca
     1561 cgattggtgt gatgccagta gacgttgtcg aacatctcct gccgggcgtt gatcagcgag
     1621 tgggtcgggc tgatcccctt ctcgccgact gtgacgcgcc gttccgcatc gacctcgtgg
     1681 atctcgaggg agctgatcaa ccgctcagtg tcgacgccgc catagggcac gcc
//
LOCUS       DATAOZ010000443         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7808316, whole genome shotgun sequence.
ACCESSION   DATAOZ010000443 DATAOZ010000000
VERSION     DATAOZ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7808316"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1647)
                     /locus_tag="VHR64_11315"
     CDS             complement(223..1647)
                     /locus_tag="VHR64_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX3303462.1"
                     /translation="MERLTEEDRFMLWPDEIWPQDIGALALLDGSRLIDSNGAFRIDA
                     VREAVASRLHLVPRFRQLLYIPPRRLGGPLWVDASTIDLADHVAIFPVPAPGNEAQLL
                     LAVEQLRRRRLDRSRPLWEMWFLTGLPEHRVGLFMRMHHAMADGMAALATLESFLDTT
                     PYVIPDPPKPWTPARMPTERDLLRDKRQRQRRQFRDALSAIAHPVGPIRYRLAAWPAL
                     RELVADRPQPVTSLHRVVGPDRSLALIRGSLQEIRHVTRACNATVNDVLLTVTAGGLR
                     VLLSSRGEPVDGVTVRIYVPVSMHREAGAHARGNLIGQMAVPLPIGIADPVARLQAIS
                     RETAQRKIRIRPSLGRMPRGIARRVLLPLVKRQRVNVASTDIPGPQMPLYLAGARLLE
                     VFPIIQLLGTVTLAVGGMSYAGQFNVMAVADAQGYPDIGIFARGVHEELRALNDAVQP
                     LGVGQPQIPAPSRSELPDASFSDR"
BASE COUNT          303 a          584 c          563 g          319 t
ORIGIN      
        1 ggacgtgcta cgcgtcgcag tcgactcagc cgaggcgcca ccagcgctcg tcgaccaggc
       61 cgatgtcatc gttgatgggc cgccgggact gctggcgttc ctacggcgat tgcttgcctg
      121 aacgatttaa cctgcggacg atgcgcatgg ccgtgcccgt cgacgcgctc agacttccag
      181 cggttgcgct gccggtgtgc atcgggttag gagggcagga cctcatcgat cactgaagct
      241 ggcatcgggg agctcgcttc tgctgggcgc cggaatctgt ggctggccca ccccaagggg
      301 ttgtaccgcg tcgttgaggg cgcgcaactc ctcgtgaacg ccgcgggcaa agatcccgat
      361 gtcgggatag ccttgagcgt cagcgactgc catgacattg aactgtccgg cgtacgacat
      421 tccgcccacg gcgagagtca cggttccaag cagctgaatt atcggaaaca cctccaacag
      481 ccgcgctccc gccaggtaca gcggcatctg cgggccggga atgtcggtgc tggccacgtt
      541 gacccgttga cgcttcacca acggcaatag gacgcggcgg gcgatcccgc ggggcatcct
      601 gccgagtgac ggccggatcc tgatcttccg ctgcgccgtc tcccggctga tcgcctgcag
      661 tcttgcgact ggatctgcga tcccgatagg cagcgggacc gccatctgcc caatcagatt
      721 cccccgcgca tgagcacctg cttcccggtg catcgatacg gggacgtata tccgaactgt
      781 gacaccgtcg accggctcac ccctgctgct cagcagcaca cgcagtccgc ccgcggtgac
      841 cgtcaggagg acgtcgttca ccgttgcgtt gcacgcccgc gttacatgcc taatctcctg
      901 aagactgccc cggatgagcg ccagactgcg gtctggccct acgacgcggt ggaggctggt
      961 aaccggttgc ggccgatcgg cgacaagttc ccggagcgcc ggccacgccg ccagtcgata
     1021 tcggatcggg cccaccgggt gtgcgatggc cgacagcgca tcacgaaatt gccgtcgctg
     1081 ccgctgtcgc ttgtcccgaa gcaggtcacg ctctgtcggc attcgggccg gagtccatgg
     1141 tttcggggga tccggaatca catacggcgt cgtatcgaga aagctttcga gggtggctag
     1201 tgctgccatg ccgtcggcca tggcgtggtg catccgcatg aaaagaccga ctcgatgctc
     1261 cggcaagccg gtaaggaacc acatctccca cagcgggcgt gaccggtcca gccgtcgccg
     1321 gcggagttgc tcgaccgcca gcaggagttg tgcctcgttg cctggagccg gcacggggaa
     1381 tatcgcgaca tgatcagcaa ggtcaatggt ggaagcgtca acccacagcg gaccccccag
     1441 ccgccgcggc ggtatgtaga gaagctggcg aaaccgtggc acgagatgga gcctgctggc
     1501 gactgcctct cggacggcat cgatccggaa ggcaccgttc gagtcgatca gcctgctgcc
     1561 gtccagcagc gccaatgcgc cgatgtcttg tggccagatc tcgtccggcc acaacatgaa
     1621 ccggtcctct tcggtcagtc gttccatcga catcacccca ggcttagctt cggtccggcg
     1681 gcgaggccgt cgcaagggac ttccgacttg tcgctgcggg tcgatcggcc cccttcctgt
     1741 cgtccactgc ccacctctgg gctggccga
//
LOCUS       DATAOZ010000444         5075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7547976, whole genome shotgun sequence.
ACCESSION   DATAOZ010000444 DATAOZ010000000
VERSION     DATAOZ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7547976"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..782)
                     /locus_tag="VHR64_11320"
     CDS             complement(270..782)
                     /locus_tag="VHR64_11320"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX3303463.1"
                     /translation="MSSEVVTEAAARGSDLAKQQPEAVSPASELGTRTESGGVSAKFA
                     HLANRTSILAGNYKTFIVMVGVVLLWAISGPIFKFSTTWQLVINTGTTIVTFLMVFLI
                     QNTQNRDSLAMHLKLDEIIRAIDAADDKLMSAEDVTEEELNELKVKYQTLVEEADQLR
                     EKVGGSREVE"
     gene            887..1534
                     /locus_tag="VHR64_11325"
     CDS             887..1534
                     /locus_tag="VHR64_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3303464.1"
                     /translation="MAATRTANPGKGSVDIPHPDVDLDNELIGRVAAGDNIALMALYD
                     RHSRQAFGLAFRILGDAATAEEVVQDAFLSLWRNAKSFDTSRGGVRTWLLTIVHNRAI
                     DRIRAAKSRASTVELEIADYAGAASDPWDDVADRLDGQQVRAAVAELPADQRSAIELA
                     YFQGLTHQEIAARTGLPLGTVKGRLRLGLRKLASALAPEPAEAGSTRDRAGPDDT"
     gene            1601..2365
                     /locus_tag="VHR64_11330"
     CDS             1601..2365
                     /locus_tag="VHR64_11330"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX3303465.1"
                     /translation="MTDTTGPTTQTHEWYQDLLGAYALGALLPDEASALEAHLATCPT
                     CPEELRRLRFGARSLALTADERDPSPDLRNRLWAATFAPADVAPRPQPARPVEPVSIE
                     HARAARRAPLPLWTKIAAALAIVLIGSLLAWNIALRNDQSGDQGTVVGQLAAAPDAPD
                     QATGGEVRYLPDDQVMLVEMHDLPTLAEGEVYQLWLIRGDAVAPAGTFQPNPSDTTTV
                     AVTGNPDQYDTLAVTREPGPIGSPAPTTKPFLVGNI"
     regulatory      2580..2663
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            2907..4049
                     /locus_tag="VHR64_11335"
     CDS             2907..4049
                     /locus_tag="VHR64_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HEX3303466.1"
                     /translation="MQIPRAGVGTPIARPSVPVAAAPPRREHVVSDGIPAIAAISVGT
                     LMFLINPGLMLATSVLVGLVLAAVLLRERQNSSNAIILVGRSPMAAMVAVTLEQQTHG
                     RGLTILRATHLAEAAALARNHACDEVVLVGPTAPQSIPLVDVRGQAPEIVPGAEKLEQ
                     LLSRVPLELAEQDRWLNRLHGARPLEAGYALAKRAIDIGVALGLSLALLPLLPLIALA
                     IKLDSRGSVFYSQDRVGLNGRLFRIYKFRTMVQDAEKHGAVWATKTDARVTRVGTFMR
                     KTRMDELPQIWNLLRGDMALVGPRPERPEFTTRLAEEIPAYDLRHAVKPGLTGWAQVC
                     YRYTSSFRDTRAKVEYDLYYVKHCSLRLDLRIMLRTFGVVLAMKGQ"
     gene            4602..>5075
                     /locus_tag="VHR64_11340"
     CDS             4602..>5075
                     /locus_tag="VHR64_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3303467.1"
                     /translation="MAGATRGTRRTPNGIATRAGLPASVGLAATSDPGPGRTSPRHGC
                     GQEEVAVLRSERSGVVRFSVRLVLATALLLMAMGNQLALAQQEFDLPELDDYSGLQAV
                     TANSVDGVNVRAEPGVDSEIVISVPDGNIVDLRVDEIRTVTTDDGIRWWPISIWGV"
BASE COUNT          857 a         1666 c         1668 g          884 t
ORIGIN      
        1 gctgaaggag aacctcgacc aggagaaggc gatgtcgaag acgctcgaga cgctctcgaa
       61 ggccgtcggc aaggattaca aggccaagac caaggccacg acggcatcaa atggcagggc
      121 cacatccgct tccggctcga aggcgaaggc aaccagcaag aagagctgac acagacttta
      181 cggcaaacaa cgaaccggac ggctctgacc gtccggttcg ttcatttcag cgcgacgcag
      241 tagatcggcg ctattactcg acttctcagc tattcgactt ctcgacttcc gccaactttc
      301 tcccgcagct gatccgcctc ctccacgagc gtctgatact tcaccttgag ctcgttcaac
      361 tcctcttcgg tgacgtcctc ggcgctcatc agcttgtcgt ccgccgcgtc gatcgcgcgg
      421 atgatctcgt ccagcttgag gtgcatcgcc agcgagtcgc ggttctgggt gttctggatc
      481 aggaagacca tcaggaaggt gacgatcgtg gtgccggtgt tgatcacgag ctgccacgtc
      541 gtcgagaact tgaagatcgg gccggagatc gcccagagca ggacgacgcc gaccatcacg
      601 atgaaggtct tgtaattgcc ggccaggatc gaggtccggt tggccaggtg cgcaaacttg
      661 gccgagaccc cgccgctctc agtcctggtg cccaactcgg aggccggcga gaccgcctcg
      721 ggttgctgtt tcgcgaggtc ggaacctctg gcggcagctt cggtgactac ttcagatgac
      781 atgtgcagat tcctccaacc tcatcagtga ggccgaacgc atgatcgcaa actctcgtca
      841 ggtcatcgca ccctgcgcta cgaatcggat tgcgaggtgc agtacgatgg cagcgacgcg
      901 aacagccaat cccgggaagg gctcggtgga cattccgcat ccggacgtgg atctcgacaa
      961 cgagctgatc ggacgcgttg ctgcgggcga caacatcgcc ctgatggcgc tgtacgatcg
     1021 tcatagccgg caggccttcg ggctcgcctt ccggatcctg ggcgatgccg ccacggccga
     1081 agaggtcgtg caggatgcct tcctctcgct ctggcgcaac gcgaaatcgt tcgacaccag
     1141 tcgcggcgga gtccggacct ggctcctgac catcgtccac aaccgcgcga tcgaccgaat
     1201 ccgggcggca aagagccgcg ccagcacagt cgagctcgag atcgccgatt acgccggcgc
     1261 cgcctccgac ccgtgggatg atgtggctga tcggctcgat ggccagcagg tgcgggcggc
     1321 ggtggccgag ctgccggcgg atcagcgcag cgcgatcgag ctggcctact tccagggact
     1381 gacccaccag gagattgccg cgcggaccgg tctgcccctg ggcacggtca agggccggct
     1441 gcggctggga ttgcgaaagc tggcctcggc gctggcgccc gaaccggccg aggcaggctc
     1501 gacccgtgat cgagcagggc cagatgatac gtaataccgg tgagcggaac gagttccgac
     1561 gggcgctttt acccagattt gcgcagattc gattggtgag atgacagaca cgaccgggcc
     1621 gacaacccag acgcacgagt ggtatcagga cctgcttggc gcctatgccc tcggggccct
     1681 cttgccggac gaggcgtccg cgctcgaggc gcacctggcc acctgcccga cgtgtccgga
     1741 ggagctgcgc cggctgcggt tcggtgcacg ctccctggcc ctgactgccg acgagcgcga
     1801 tccctcgcct gatctgcgca accggctctg ggcggcgacg ttcgccccgg ctgatgtcgc
     1861 gccccggccg cagccagccc ggccggtcga gccggtctcg atcgagcatg cccgggccgc
     1921 gcgcagagct ccgctgccac tctggacgaa gatcgcggcg gcgctggcga tcgtcctgat
     1981 cggcagcctg ctggcctgga acattgcgct gcggaacgac cagtcaggag atcagggcac
     2041 ggttgtcggc cagctcgcgg ccgcgcccga tgcgccggac caggccaccg gcggcgaggt
     2101 gcggtatctt ccggacgatc aggtgatgct tgtcgagatg cacgatctgc ccacgctggc
     2161 ggagggcgag gtctaccagc tctggctgat ccggggagac gccgtcgcgc cggcgggcac
     2221 cttccagccg aacccgagcg acaccaccac agtcgccgtc accggtaatc ccgaccagta
     2281 cgacactctg gccgtcaccc gcgaacccgg cccgatcggc tcccctgcgc cgacgacgaa
     2341 gccctttctg gtcggcaaca tctgagcggg gaagggttga tggtagttgg tggatggaat
     2401 tcacatccaa ccaccatcca ctaaccacca accactggac acaagtaccg tggtaccggc
     2461 ttgaatggcc ggatactcct aggtacgact gctgcgctgc tcccccgttc ttcctatcgt
     2521 gcttgtagga gattggtccc ggtgccgggg gatgaccccg gcaccggaat caaatcccac
     2581 cggacaacgg atgcgctgat ccaccgcctg tacctggtca cctgggcgat ggggagctag
     2641 tggtgaaacc ggccgccttc gagaggagcc ggtttttgct tgctcgcgat tgctcggcgc
     2701 ccgtggccag cccacgtccc gatgcacgcg gtggatggcg aagcacaggg ccaaatcccc
     2761 tgctttcgcc gacggatcgg gtcccggtcc gatcccaggc tcgtttgtcg gtcgctccga
     2821 tccccccgga gtgaccggca gcgggtctcc cccgcctcgc gaaccggcaa cgaccggctc
     2881 tgtcgctgga gcgcggagtc gttcagatgc agattccccg agcaggcgtg ggcacgccga
     2941 tcgcccggcc ttccgtcccc gtcgccgcgg cgccgccccg ccgcgagcac gttgtcagcg
     3001 atgggatccc ggcgattgcc gcgatcagcg ttggcacgct gatgttcctg atcaacccgg
     3061 gcctgatgct cgccaccagc gtgctggtcg gcctggtgtt ggccgcggtc ctgctgcggg
     3121 agcgccagaa cagctcgaac gcgatcatcc tggtcgggcg atcgccgatg gccgcgatgg
     3181 tcgccgtcac actcgagcag cagacgcacg ggcggggact gacgatcctg cgcgccacgc
     3241 acctggctga ggccgccgcg ctggctcgca atcacgcctg cgatgaggtg gtgctggtcg
     3301 gcccgaccgc accgcaatcg atcccgctgg tcgacgtccg cggccaggcg ccggagatcg
     3361 tgccgggcgc cgagaagctg gagcagctcc tgagccgcgt gcctctggag ttggccgagc
     3421 aggatcgctg gctgaaccgc ctgcacggcg cccgcccgct cgaggcgggc tatgcgctgg
     3481 ccaagcgcgc gattgatatc ggcgtggccc tcggcctctc cctggcgctc ctgccgttgc
     3541 tgcccttgat tgccctggcg atcaagctcg attcgcgcgg ctccgtcttc tactcgcagg
     3601 atcgggtcgg gctgaacggc cggttgttcc ggatctacaa gttccgcacc atggtccagg
     3661 atgccgagaa gcacggcgcc gtctgggcga ccaagaccga cgcccgggtg acgcgggtcg
     3721 gcacgttcat gcgcaagacg cggatggacg agctgccgca gatctggaat ctgctccggg
     3781 gcgacatggc gctggttggt cctcgcccgg agcggccgga attcacgacc cgcctggccg
     3841 aggagattcc ggcctacgat ctgcggcacg cggtgaagcc gggcctgacc gggtgggcgc
     3901 aggtttgcta ccgctacacg agctcattcc gggacacccg cgccaaggtc gagtacgacc
     3961 tctattacgt caagcactgc tctctccgcc tcgacctgcg gatcatgctg cgcaccttcg
     4021 gggtggtcct ggcgatgaag gggcagtagg cgggttgagg gttgaggcaa cgaaacgcgg
     4081 gtagatggta aatggcggtt ggttgatgga gcaggaactc acgtagcgtc gcatggcaat
     4141 gcgacctgat gtcccggtga ccggtgcgat gcatggagcg gtgtctgccg gtgccgcccg
     4201 gggataaatg cccgggctac accacgcatg ggcctcatca tcccggaacc gaggtcggat
     4261 tgccatgcga cgctacaact gctcgcctcg tctgttgttg cctgcaacgc cgcgtctggc
     4321 aattcaatct tgctcaaatt tttgcaaatc catgtgtacg taccctaggc aggggagatt
     4381 ttgctctggt acgctgcgct cgggtgcccg gcgcgtccgt acaacttgct ccgtgatcct
     4441 gatcgggatc atcggtgctg tcctgtccga acacggtgtc cgattagctt tcggcccagt
     4501 tcggtatcct gtggcggaac ctcccgcacc cgcgtttcga cgcaggagac aggccagcgc
     4561 ccgctgatgc tgacagtcag gctcagtacg ccgggatggc ggtggccgga gcaacgcgag
     4621 gaacgcgccg tacacccaat ggcatcgcga cccgggccgg attgccggca tcggtcgggc
     4681 tggcggcaac gagcgatccc ggtccagggc ggacttcccc gcgccatggc tgtggacagg
     4741 aggaagtggc ggtgctcagg tctgagcgct ccggtgtggt gcgattttcg gtgcggctgg
     4801 tgctggccac cgcgctcctg ctcatggcga tgggcaacca gctcgcgctg gcccagcaag
     4861 agttcgatct cccggaactg gatgactaca gcggcctgca ggccgtcacg gccaacagcg
     4921 tcgatggcgt gaacgtgcgc gctgagccgg gcgtcgacag cgagatcgtc atctccgtcc
     4981 ccgacggcaa catcgtcgac ctccgcgtcg atgagatccg caccgtcacc accgacgacg
     5041 gtatccgctg gtggccgatc tcgatctggg gggtc
//
LOCUS       DATAOZ010000445         9949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4915693, whole genome shotgun sequence.
ACCESSION   DATAOZ010000445 DATAOZ010000000
VERSION     DATAOZ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4915693"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VHR64_11345"
     CDS             complement(<1..302)
                     /locus_tag="VHR64_11345"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX3303468.1"
                     /translation="MRVNQYIRGGGEPGTAEHVLHAFDRWPTWMWANWEIVALAEWIR
                     SHNDDRPEQERVGLYGLDVYSLWESLSAVVEYLRRVTGAAMGAVVGRRGTGNKGGS"
     gene            complement(349..615)
                     /locus_tag="VHR64_11350"
     CDS             complement(349..615)
                     /locus_tag="VHR64_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303469.1"
                     /translation="MLDAFWHIKSAHGNQNRMHRLSRRAQPETDESLGQQVLTLSSPL
                     VDSGYLDPLHERIGDARYVLLGEASHGTHDYYLWRARISRRLSR"
     gene            820..1719
                     /locus_tag="VHR64_11355"
     CDS             820..1719
                     /locus_tag="VHR64_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303470.1"
                     /translation="MRRAVGFVAILCSLLMSGSAISMAQDATPASSAALSGYPEVKVT
                     ITDQAIEVDKTDVPAGYVLLTVTNSSKDENSAGVLGPGPGKTMEDLKQAAATPASGDE
                     IPPFLYEATVLGGPGDIQPGQTAQVVINIPAGDWAVFPEGNQQPVMITASDSADSNTT
                     APTADLSIDLGDFSFSGFSDGVAAGQQLWKVTNSGKQIHMVVIGKVPEGTTEQQVLDT
                     FTAEDTGTPVPGALQESDFQPTSTGVLLLSSGQSIWVPANLDNGTYVALCFVTDPQTG
                     KVHAMEGMISVFQVGGGTSTPTS"
     gene            1836..2531
                     /locus_tag="VHR64_11360"
     CDS             1836..2531
                     /locus_tag="VHR64_11360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX3303471.1"
                     /translation="MHPDFPETGIEVARKEIKHLYIRVDRQDGRIRVSAPRRMSDAAI
                     HEAIRRNRSWIERRRQRIATQPKIEAPALETGTCVLVFGEPRRLAIVTGSGHRGVRLR
                     EDGVLEMRMHSEGDHGQREALLDTWYRAQLQNRIPELIRTWEPVMGVSVAEWGVRKMR
                     TRWGSCNIRARRIWLNLELVRRPISCLEYVVVHEMVHLLERGHNRRFYKFMDKFLPDW
                     RDARALLNTAPPG"
     gene            2680..4551
                     /locus_tag="VHR64_11365"
     CDS             2680..4551
                     /locus_tag="VHR64_11365"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEX3303472.1"
                     /translation="MLEHDAGRPSGTADERAIDLSLTRRTLLLGLGTAAVGVAADGSA
                     AAQDAATPVAESPVAPQGDEIWVYPVPGSVTASAGTQISFRGTGLQSLDLIEVIGSKS
                     GYHSGVQIAHSDGNGFSFVPDFGFRAGEEVAVRTRVRITNAKAKRFTFTVAESRPLPA
                     SPELNEKADEDLVHSYRSRPGLAPPVIEAVAHADGTSPGYLFLAPKRGTGQNGALIVD
                     DTGEPVWFNPVKVAAEEVYDLRVNQFMGQTVLTWWQGITVSGHGFGHWVICDEHYQQV
                     ATVRIGNGFAGGDMHDFVLTPENTAIIGTYNTLHWDLSSVGGVPDGYAIDGIIQEIDI
                     STGAVMFEWHSLDHVPVDESYSKIDPEQPGDFDYFHLNSVAVDSDGHLIINARNSWAA
                     YKVNRTSGKILWRYCGRQSDFKMGDGTEVAYHHDARPHGNGLISFFDNGGSPKVHDES
                     RGLIVQLDMDKMTASLVHEYVHPDKISASSQGNTQLLPNGNVLVGWGSEPIASEFSED
                     GTLLHDWRMPKEKQSYRAYRFEWTGKPSAGPSLVVDSESGDTVAYVSWNGATEVRAWR
                     IKTGTDRESLEPDIDTVEKTGFETEISIDTSSGFLAVEALDATGQVIGTTKIVELNG"
     gene            4577..6634
                     /locus_tag="VHR64_11370"
     CDS             4577..6634
                     /locus_tag="VHR64_11370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3303473.1"
                     /translation="MSLTTTESSVGLSESEPAIEVFAAGTDADPSTHYVYLVLIFVVT
                     VVAAVLRFANLGEVGFRIDEGFTMTFARQPWAGVLGLHGYYDLHPPLFFSLAKFADLF
                     VPETIASRVVAATSGVATIPVFYGFCRRLIDERVALGAALLLTVSPLHIEFSRDGRMY
                     APVIFCVVVGWLALVAYLQTRRPTWAVTYGLSLLLAMYIDYSAVYALVPQAVVLAIYL
                     VRVRGRDPWLVGSLLGALIGYVPWLPQVYRSVAKSRHHAGRADWLSASWDRIGESIPS
                     LLGMMGRTVTSGGDNRGTWGRLPDWHPVLLLIAFAALAVGLVALRRWPRVAMFVVMLV
                     VLPPLAAIVLSQISPGYAPRTIMASVLGLTILASAFLARGQIGRFVRGFGAVGWAMFL
                     TISLITLPSTYSAGTRTEWPDIAHDLAAQSSMNHPVLVFSTAGMLTDMLDLYGGDRLA
                     GTRIITLLDGPREEEIGYQRWLDRGPQLREIQQGALAGLLPQSDPANDVIWVILRFGG
                     GKIPQYLAQIGYTQIGSIRYTDTDLLLFARPGAQIGSPVQMDPRYSEATGNSDGWRYA
                     NKNVAVRAAGAIWEMVLTGDRSVSTYRFTTQSGGLVVGRIDAQVPAGSLAVTVSCVSA
                     NGDVLRSSRATSQKNFADWQSISVAGLCPDRTATVEVRFQRRGEGSVILRNAQLEFNP
                     NQN"
     gene            complement(6690..7520)
                     /locus_tag="VHR64_11375"
     CDS             complement(6690..7520)
                     /locus_tag="VHR64_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303474.1"
                     /translation="MTRSRMYALTAAAAGLYIFLCWAFADGLFSLSGNALWNSDAIVE
                     STIWTYVMLAAIVVAGLYEASKIPVEGVPVTVSRDVSPGQMDDPVFWKLAMGSTYYAL
                     LWLPLRFFVGREWAAAGEHKMRDDAWMKGGSALKGYWERATTVPEGRPTSPSGTYAWF
                     EDFLKYMLDHEWYTWFAKVIAVGEFLVGIGLIVGALVGIAAFFGTLMNFNFQLAGSAS
                     TNPVLFGLGVFLVLGWKVAGWIGLDRYLLPALGTPWKRGRLLEHESYESAGPGHAMRA
                     "
     gene            complement(7698..>9949)
                     /locus_tag="VHR64_11380"
     CDS             complement(7698..>9949)
                     /locus_tag="VHR64_11380"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX3303475.1"
                     /translation="PLAAEHIKPRLLGHWGTSPGLDLIYVHMNRLIRERDVNAIYLAG
                     PGHGGPAIVAQIYLEGTYSEIYPQITMDLDGMRLLFRQFSTPGGIPSHVSVQTPGSIN
                     EGGELGYVLSHAFGAAFDNPDLIVVAVVGDGEAETGPLSGSWKSTRFLNPARDGAVLP
                     ILHLNGYKIASPTVSARSADDEIGKFLESQGYRMHVVEGDDPASVHQQMASVLEACHD
                     EIRAIQDDARRNGMTDRPAWPAIVLRTPKGWTGPEVVNGLPVEGTFRSHQVPLSKVGS
                     DEAQLAALEAWMRSYEPEEQFDNDGALVPELSDLAPSGDRRMGANPVANGGRVLVELN
                     MPDFTEYAVALERHGASREENTRPLGKLLRDIFVQNKRSQNFRVFCPDELASNRLDDI
                     LNVETRCYVGPLLEIDSGLAHDGRVMEVLSEHLCEGWLEGYLLTGRHGLYATYEAFAM
                     VSASMTIQHTKWLQEAGNLSWRDPVASLNILLSSTCWRNDHNGFSHQGPGLIDVMLSK
                     RGTVARIYLPPDANCLLSVADHCLRSRNYVNLIVADKQRHLQYLSMDEAIEHCAAGAS
                     TWHWASSDDGQEPDVVLACAGDVPTLEALAAADWLRERLPDLRVRFVNVVDLMTLFPQ
                     EFHPHGMSETRFVELFTADKPVVFAFHGYQRAIHEIVHGRVHAERFHVRGFNEQGTTT
                     TPFDMTILNRMSRYHLAREALRRVRRVYSTAEGLMPELDALISKAVAYTRENLQDPPE
                     IRDWTWERG"
BASE COUNT         1902 a         3031 c         3083 g         1933 t
ORIGIN      
        1 atgctgcctc ccttgttgcc agtgccgcgg cggccgacga cggcgcccat cgcggcgccc
       61 gtgacccgcc gcaggtactc gacgacagca gagagcgatt cccagagcga ataaacgtcg
      121 agcccgtaga gaccgacgcg ctcctgctcg gggcgatcgt cgttgtggga ccgtatccat
      181 tcggccaggg ccacgatctc ccagtttgcc cacatccagg taggccagcg gtcaaacgcg
      241 tggaggacat gctccgcggt cccgggctcg cccccgcccc ggatgtattg gttaacccgc
      301 aagcaatcgg gccagtcgcc ctcgactgcg atgaagctga accccttctc atctactgag
      361 acgacggctg atccgggccc gccagaggta atagtcgtgc gtaccgtgcg atgcctcccc
      421 cagcaacaca tagcgagcgt cgccaatgcg ctcgtgcagg ggatcgaggt agccgctgtc
      481 gacgagcggt gaggagaggg tcagtacctg ctggccgagc gattcgtccg tttctggctg
      541 tgccctgcgg gagagtcgat gcatgcggtt ctggttccca tgagcagact tgatgtgcca
      601 gaacgcgtcc agcactggcg acgctcccat cctagtcagc acttcgacgt gatcacagcg
      661 ccgatttgac gccgcgctac gagcttcgca aggcgaatag aagtcgatgt agcgtcgttt
      721 cgtattactc gtagtacgat atgaatccaa ttgcgcattc tcgtcgtcta actcaccacc
      781 tgtgctttta caggggagta atgcgcttgg aggacagaca tgcgccgtgc agttgggttc
      841 gttgcgattc tctgttcatt gctgatgagc ggctcggcca tttccatggc gcaggacgcc
      901 acgccggcaa gctctgcagc gctaagtggc tatccggagg tgaaggtcac gatcaccgat
      961 caggcgatcg aggtcgacaa gaccgacgtg ccggccggat acgtcctgtt gaccgtcaca
     1021 aactcgtcga aagacgaaaa cagtgcgggc gtgcttggtc ctggaccggg caagacgatg
     1081 gaagacctga agcaagcggc cgcgacaccg gcgtcgggag atgaaattcc gccgttcctg
     1141 tacgaggcga ccgtgctcgg tggacccggc gatattcaac cgggtcaaac ggcgcaggtc
     1201 gtgatcaata tcccggcagg cgattgggcg gttttccccg aaggtaacca gcaaccggtc
     1261 atgatcactg cgtcagattc ggccgactcc aacaccaccg cgccgaccgc ggatctctcg
     1321 attgacctcg gcgacttctc gttctccggc ttctcggatg gcgtggccgc aggtcagcag
     1381 ctgtggaagg tgacaaactc gggcaagcag attcacatgg tcgtgatcgg aaaggtgccg
     1441 gagggaacga cggagcagca agtcctcgac acgttcaccg ccgaggatac ggggactccg
     1501 gtcccagggg cgttgcagga gtccgacttc caaccgacat caaccggcgt gctcctgctg
     1561 tcgtccggcc agtcgatctg ggtgccggca aacctggaca atggaacgta cgttgccctc
     1621 tgctttgtca cagatccgca gaccggcaag gttcatgcga tggagggcat gatttcggtc
     1681 ttccaggtcg gcggcggcac atccacaccg acttcgtaag cattgatctg atctcgaaac
     1741 cggggtggga ttgtcgtccc gccccggttt tcgttgctcg ggtaggattc tgaacctgtg
     1801 aaccgacgcc gatggcattc gccggcaggg ctaccatgca tcccgatttc ccagagactg
     1861 gcattgaggt cgcgcggaag gagatcaagc acctctacat ccgcgtcgac cggcaggatg
     1921 gacgaatcag ggtgtcggcg ccgcgtcgca tgtccgacgc ggcgattcat gaggccattc
     1981 gccgcaatcg atcatggatc gagcgacgcc ggcagcgaat cgcaactcaa ccgaagatcg
     2041 aggctcccgc gctcgaaaca gggacatgcg tcctggtatt cggcgagccg cgcagattgg
     2101 caattgtgac cgggtcgggc catcgaggtg tgcgtcttcg cgaggacggc gtgctcgaaa
     2161 tgcgtatgca ctcggaagga gatcacggtc agcgggaggc gctcctcgat acctggtacc
     2221 gggcacagct ccagaatcgt attccggagc tgattcggac ctgggaaccg gtcatgggcg
     2281 tctccgtggc cgaatggggt gtccgcaaga tgcggacccg gtggggtagc tgcaatatcc
     2341 gcgcccggcg aatctggctc aatctcgaac ttgtccgaag accgatttcc tgcctcgaat
     2401 acgtggtcgt ccacgaaatg gtgcacctgc tcgagcgcgg gcataatcgc cgcttctaca
     2461 agttcatgga caaatttttg cccgattggc gggatgcaag agcactcctg aacacagcgc
     2521 cgcctggata gagtggtatt ctgcccggcg ttgcccagac cgttgcgttc ccattcgatc
     2581 gatcgtcaca tcgatttcgc agttggttgt cgatcgcgac gtgatcgcgc gtggcggcgc
     2641 cggcggaccg ccgaatcgaa cagaagagga gatccagcaa tgctcgagca cgatgcaggc
     2701 aggccgagcg gaactgctga cgaacgtgca atcgaccttt cgttgactcg tcgcacgctc
     2761 ttgctgggtc tcggaacagc cgcagttggc gtggccgcag acggaagtgc ggcggcgcaa
     2821 gatgcagcca cgccggtagc ggaatccccg gttgcgccgc aaggcgacga gatttgggtc
     2881 tatcccgtgc cgggctcggt cacggcatcg gccggaacgc agatcagttt ccgcggcacg
     2941 ggtctgcaat cgctcgacct catcgaagtc atcggatcga agagtggata ccactccggc
     3001 gtacagattg cgcattcgga tggcaacggc ttcagcttcg ttcccgactt cggttttcgg
     3061 gcgggcgagg aggtcgccgt tcgaacgagg gttcggataa ccaacgcgaa ggcgaagcga
     3121 tttacgttta cggtcgcgga atcgcggcca ttgcccgcta gccccgagct caacgagaag
     3181 gcagacgagg acctggtgca ctcgtaccgc agccgtcccg gcttggcgcc gccagtgatc
     3241 gaggccgtcg cgcacgcgga tggcacgtcg cccggatatc tcttcctagc tccgaaacgc
     3301 ggaaccggcc aaaacggcgc gctgatcgtc gatgacaccg gcgaaccggt ctggttcaat
     3361 ccggtcaagg ttgcggccga agaggtctac gacctccggg tcaaccagtt catgggccag
     3421 acggtgctga cctggtggca ggggatcacc gtctccggac atggcttcgg acactgggtg
     3481 atctgcgacg agcattacca gcaggtggcg accgtccgca tcggcaacgg attcgccggc
     3541 ggcgatatgc acgatttcgt gctgacgccc gagaacaccg ccatcattgg gacctacaac
     3601 acgctgcatt gggatctctc gagcgtcggt ggggtgccgg acgggtacgc aatcgatggc
     3661 atcatccagg agatcgacat ttccaccggt gcggtcatgt tcgagtggca ttcgctcgac
     3721 cacgttccgg ttgacgagtc gtacagcaag atcgatccgg agcaacccgg cgacttcgac
     3781 tacttccatt tgaactctgt cgctgtcgat tccgacggcc atttgatcat caatgcgcgc
     3841 aacagttggg ctgcgtacaa ggttaaccgg acctcgggca agatactctg gcggtactgt
     3901 ggacggcagt cagatttcaa gatgggtgac ggtacggagg tcgcgtacca ccatgacgcc
     3961 cggccacatg gcaacggcct gatctcattc ttcgacaatg gcggatctcc gaaagttcac
     4021 gacgagtcgc gcggactgat cgtccagctc gatatggaca agatgacggc gtcattggtc
     4081 cacgagtacg tgcatccgga caaaatatcg gcgagcagcc aggggaatac gcagctgctt
     4141 ccgaacggca acgtgctggt tggctgggga agcgagccga ttgcctccga gttttccgaa
     4201 gacgggactc ttctgcatga ttggcgcatg ccgaaggaga agcaatccta tcgggcgtat
     4261 cgattcgagt ggactggcaa gccgtccgct ggcccatcgt tggtggtcga ttcggagtcg
     4321 ggtgataccg tcgcgtatgt gagttggaac ggcgctactg aggttcgggc ctggcgcatc
     4381 aagaccggca cggatcgcga atcgctcgag cctgatatcg acacggtaga gaagactggc
     4441 tttgagaccg agatttccat cgataccagc agcggattcc ttgcggtcga ggcgctcgat
     4501 gcgactggcc aggtcatcgg cacaacgaag atcgtcgagt tgaacggcta aggggtagtt
     4561 catcgggcca ccggcgatgt ccctcacaac gaccgagtcc agtgtcgggc tgtcagaaag
     4621 tgaaccggcg atcgaggtgt ttgcggccgg aaccgacgca gacccgtcga cgcactacgt
     4681 ctaccttgtg ttgattttcg tcgtgaccgt cgtagcagcc gtgctgagat tcgcgaacct
     4741 gggtgaggtc ggattccgga tcgacgaggg gttcacgatg accttcgccc gccaaccatg
     4801 ggcgggcgtg ctcggtctgc acggctacta tgatctccac ccgccgctct tcttttcgct
     4861 ggccaagttc gcggatctgt tcgtgcccga gacaatcgcg tcccgggtcg tcgcggcgac
     4921 ttccggcgtc gccacgattc cggtcttcta cggtttttgc cggcgactaa tcgatgagcg
     4981 agttgcactc ggagcggctc tgctcctgac ggtatcgcca ctgcacatcg agttctctcg
     5041 cgacggacga atgtatgcgc cggtcatctt ctgcgtcgtc gtcgggtggc tcgccctcgt
     5101 cgcgtatctg caaacgcgcc ggcctacctg ggcagttacc tacggcttga gtctgctgct
     5161 cgccatgtac atcgactaca gcgccgtcta tgcgctcgtc ccgcaggcag tcgtgctcgc
     5221 gatctacctg gtgcgcgtgc gcgggcgcga tccctggctg gttggttcgc tgctcggcgc
     5281 cttgatcgga tacgtgccct ggcttccgca ggtctaccgg tcggtcgcga aatcccggca
     5341 tcacgcgggc cgcgccgact ggctgagcgc ttcgtgggat cggatcgggg agtcgatccc
     5401 atcgctcctg ggcatgatgg gccggaccgt tacctccggt ggagacaacc gcgggacctg
     5461 gggacggctc cctgattggc atcccgtact gctgctgatc gcgttcgcgg cacttgctgt
     5521 cgggctcgtt gccctgcgga gatggccacg agtggcaatg ttcgtggtca tgctggtggt
     5581 gcttccgccg ctcgccgcca tcgtcctcag tcagatcagt cctggttatg ctccccggac
     5641 gatcatggcg tcagtgctgg gtctgacaat ccttgccagc gcattcctgg cacgcggaca
     5701 aattgggcga ttcgtccgcg ggtttggtgc ggtcgggtgg gctatgttcc tgacgatctc
     5761 gctgataacc ctgccctcca cctattctgc cggcacgcgg actgagtggc cggacattgc
     5821 ccacgatttg gcggcccaga gctcgatgaa ccacccggtg ctggtgtttt cgacggccgg
     5881 aatgctgacc gacatgcttg acctctacgg tggcgacagg ctggccggaa cgcggatcat
     5941 cacgttgctc gatggtccac gagaggaaga gatcggatat cagcgctggc tcgaccgcgg
     6001 accgcagttg cgcgaaatcc agcagggcgc ccttgccggt ttgttgcctc agtcggatcc
     6061 ggcaaacgac gtgatctggg tcatcctccg attcggtggc gggaagattc cgcaatacct
     6121 cgcccaaatt ggctacaccc agatcggctc gattcgatac accgataccg acctccttct
     6181 cttcgcacgg ccgggcgcgc agattggatc acccgtgcag atggatccgc ggtactccga
     6241 agcgaccggc aacagcgatg gctggcgata cgcaaacaag aacgtcgcgg tccgtgcagc
     6301 gggcgcgatc tgggagatgg tgctcaccgg agatcggagc gtaagtacct atcggttcac
     6361 gacgcagagc ggaggccttg ttgtcgggcg catcgatgcc caggtgcccg ccgggtcatt
     6421 ggcggtcacg gtttcatgtg tatcggcaaa cggcgacgta ttgcgctcaa gccgggccac
     6481 gtcgcagaaa aatttcgcgg actggcaatc gatctccgtt gcaggactct gcccggaccg
     6541 aacggccacg gtcgaggtga ggtttcagcg ccgtggagag ggcagtgtga ttttgcggaa
     6601 cgcacagctc gagttcaatc cgaaccagaa ttgagccaaa aagaacgccc cctgcacgaa
     6661 gcagggggcg ctgggcggtt gcctgaccgt taggcccgca ttgcgtgacc aggtccggcg
     6721 ctctcgtagg actcatgctc gagcaatcgc ccgcgcttcc acggagtgcc cagggccggc
     6781 agcaagtagc ggtccaggcc gatccaacca gcaaccttcc aaccgaggac caggaagact
     6841 ccgagaccga acagtaccgg gttggtgctc gcggagcctg ccaactggaa gttgaagttc
     6901 atcaacgtcc cgaagaaggc tgcgatgccg acgagtgccc cgacaatcag tccgattccg
     6961 accaggaact cgccaaccgc gattaccttg gcgaaccagg tgtaccactc gtggtcgagc
     7021 atgtacttga ggaagtcctc aaaccatgcg tacgtgccgg agggagaagt cggacgacct
     7081 tccggaaccg ttgtcgcgcg ctcccagtag cccttgagcg cagagccgcc cttcatccag
     7141 gcatcgtcgc gcatcttgtg ctctccggca gcagcccact cacggccgac gaaaaaccga
     7201 agcggaagcc agagcaacgc atagtaggtg ctgcccatag ccagcttcca gaacacgggg
     7261 tcgtccatct ggcccgggga gacgtcacgg gaaaccgtga ccggcacgcc ctcgaccggg
     7321 atcttggacg cttcgtacag gccggcgacg acaatcgctg ccagcatgac atacgtccag
     7381 attgtggact ccacaatggc gtcactgttc cacagagcgt tgccgctcag tgagaacagt
     7441 ccgtccgcga aggcccagca caggaagata tacaaccctg ccgcggccgc cgttaacgcg
     7501 tacatacgac ttctggtcat ctcacacctc catccggtct ggcaccgccc gtgcgttacc
     7561 gttccatctg ttgcaagcag agtacgtttc cagtcgtgac gccctcgtaa atcactcgtt
     7621 gcaaacattg caacttcatc acattcctaa tccctccgtt tgacgatgtc aactaaattc
     7681 ctgattcccc tgacgtgcta gccgcgttcc catgtccaat cccggatttc ggggggatcc
     7741 tggaggtttt cgcgggtata ggcgactgcc ttgctgatca gcgcatcgag ctccggcata
     7801 agcccctctg ccgtcgaata gacccggcgg acccgccgca gcgcctccct ggcaaggtga
     7861 tagcggctca ttcgattgag tatcgtcatg tcgaacgggg tcgtggtcgt accttgctcg
     7921 ttgaatccac gtacgtgaaa ccgctcggca tgcacccgcc cgtggacgat ctcatggatc
     7981 gcccgctggt aaccgtggaa ggcaaaaacg accggcttgt cagcagtgaa gagctcgacg
     8041 aagcgtgtct ccgacatgcc gtgcggatga aattcctgcg ggaacagcgt catcaagtcg
     8101 accacgttga cgaatcggac cctgaggtcc gggagacgct cacggagcca atccgcagcc
     8161 gccaatgcct ccagcgtcgg cacatctccc gcgcaggcaa ggaccacgtc tggctcctga
     8221 ccatcatcag aacttgccca atgccaggtt gacgcgccgg ccgcgcaatg ctcgattgcc
     8281 tcgtccatgc tcagatactg caggtgccgc tgcttgtccg cgacgatcag attgacgtaa
     8341 ttgcggctgc gcaggcaatg atcggcaact gagagcaagc agttcgcatc cggtggcaaa
     8401 tagattcggg cgacggtgcc ccgcttggac agcatcacat cgatcaggcc cggcccctgg
     8461 tggctaaagc cgttgtggtc gttgcgccag caggtcgaac tgagcaggat gttcagcgag
     8521 gcgacagggt cgcgccagga gagattgccg gcctcctgca gccattttgt gtgctggatc
     8581 gtcatcgacg ccgagaccat tgcgaaggcc tcgtaagtcg catacagccc gtggcggccg
     8641 gtcagcagat acccctcgag ccagccttcg cagagatgct cgctgagcac ctccatgaca
     8701 cgtccgtcat gggccaggcc gctgtcgatc tcgagcaagg ggccgacata gcagcgggtt
     8761 tcgacgttga ggatatcgtc gagccggttc gacgccagct cgtccgggca gaagacgcgg
     8821 aagttctgcg accgcttgtt ttgcacgaag atatcgcgga gcagcttgcc cagcggtcgg
     8881 gtgttttcct cccgtgaggc accgtgccgc tcgagcgcta ctgcatattc cgtgaaatcg
     8941 ggcatgttga gctcgacaag tacgcggcca ccattcgcga ccgggttcgc ccccatacgg
     9001 cgatcgccgc tcggagccag atctgacaat tccgggacca gcgcaccgtc gttgtcgaac
     9061 tgctcctcag gttcgtagct ccgcatccac gcttcaagcg cggccaactg tgcctcgtcg
     9121 ctcccgacct tcgagagggg cacctggtga gagcgaaacg tcccttcgac cggtaacccg
     9181 ttgacgacct cgggaccagt ccagcccttt ggcgtacgca ggacgatcgc cggccacgcc
     9241 ggtcgatcgg tcatgccatt ccggcgcgcg tcgtcctgaa ttgcgcgaat ctcgtcatga
     9301 caggcttcga ggaccgatgc catctgctga tgcacgctgg caggatcgtc gccctcaact
     9361 acgtgcatcc gatacccctg gctctccagg aacttgccga tctcgtcgtc ggcggaccgc
     9421 gcggagaccg tcgggctggc gatcttgtat ccattcaggt ggagaatcgg aaggactgct
     9481 ccgtcccgcg cgggattcag gaagcgggtc gatttccagg agccggacaa cggcccggtt
     9541 tccgcttcgc catctccgac caccgcaacc acgatcaggt cgggattgtc gaaggccgcg
     9601 ccgaaggcat ggctcaggac gtatccgagt tcaccgccct cgttgatcga gcctggggtt
     9661 tggacactga cgtggctcgg aattccgccg ggcgtcgaga actggcggaa caggagccgc
     9721 atgccgtcga ggtccatcgt gatctgcggg taaatctcgg aataggtgcc ttcgaggtag
     9781 atctgggcca cgattgccgg tccaccgtga ccgggccccg ccaggtagat cgcgttgacg
     9841 tcgcgctccc ggatcagtcg attcatatgg acataaatca ggtccaagcc cggtgaggtg
     9901 ccccagtgac cgagcagtcg tggcttgatg tgctcggcgg cgagcggtt
//
LOCUS       DATAOZ010000446         6118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6229072, whole genome shotgun sequence.
ACCESSION   DATAOZ010000446 DATAOZ010000000
VERSION     DATAOZ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6229072"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VHR64_11385"
     CDS             <1..337
                     /locus_tag="VHR64_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303476.1"
                     /translation="DPIARVDGNGTHANRIPFDTGREMPIAVLWIRPEQQANTGLAAD
                     LGCELYDAGLITGLVKVEGPGQTTIPGVFAAGDIASPMHQVSLAVASGTAAALGATRL
                     LIQMDNALE"
     gene            412..2130
                     /locus_tag="VHR64_11390"
     CDS             412..2130
                     /locus_tag="VHR64_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX3303477.1"
                     /translation="MRQFEGRGADILAECLIEAGIDLIFGVPGDTGVVFYDALYQRTD
                     RIRHILTRDERHAGFMADGYARWTNRAGCCEVSSGGGVDFLVSGLGEAYAASVPILAI
                     TSDIHRRSRGTSAITEIDQEQLFSAVTKLAMTIETAAQIPDIIRVLLQAATTGRPGPV
                     VAIFPEDVFDEHASVTIPEMSATVPLDREPASARATHKAAELLAGAERPAIVAGGGVH
                     LSSAWPELRALAEKSGIPVATTIHGKGAIAETHPLSLGVVGANGARPFANAYLSEADA
                     VLFVGTRANSTDTNGFTSPPREGPQIIQIDIDTNRAGVNFPQSHDLVGDAKTTLAAIT
                     EVIPENPQRAESLAAQIGEWRTAWRLAEVEPRRLLEADLLDPRDVVITLQEVLPADVV
                     ILAEPGTPTPNVSAYWECPEPGRTVIDPRGHGPMGYVIPAAMGVSMSDPERPIVGLCG
                     DGSFAMACGELETANRLQLPVLYVQFTNNSLGWIKMLQHLYLGQRYFSVDPGPMNYVG
                     VAQAMGLEAIRVNDLGQLREAVREWLTHRRPTFIDVPVPDEISLTPPVAPWQAALEGA
                     TGRPVY"
     gene            complement(2528..2752)
                     /locus_tag="VHR64_11395"
     CDS             complement(2528..2752)
                     /locus_tag="VHR64_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX3303478.1"
                     /translation="MIKTTIHIPEEEMAALEAIARREDRPKAAVIREAIGRYVTENSR
                     PRPEAVGIFDDTEVDSTNLDEWLRENWNPV"
     gene            complement(2762..3370)
                     /locus_tag="VHR64_11400"
     CDS             complement(2762..3370)
                     /locus_tag="VHR64_11400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX3303479.1"
                     /translation="MSVEESSLPAALLVIDVQEAMFGPEQQPPVFRADETVSNIAALL
                     AKARTAGARVIYIQHHDQTDPALQPGQPGFAIRVVIAPKPDETVIVKRICDAFTGTNL
                     EQVLRDAGIQRLVTCGIQSDFCVDAATRGAMNRGFAVTLAADAHTTCDNGILTAEQII
                     AHVNATLPNMSGPGQRVCIKSTQEIEFGAVTPWVRGATCGMW"
     gene            complement(3383..3958)
                     /locus_tag="VHR64_11405"
     CDS             complement(3383..3958)
                     /locus_tag="VHR64_11405"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX3303480.1"
                     /translation="MSEQSKPTALVVIDVQGAMMDDYPDDPSPFRKDEVLANIGTMLE
                     NARAAGSKVIFVRHVESKYPLMSPGHPAFDVHPAIAPLEGETVIDKTACDSFCATDLE
                     PILREAGVTHLVTCGMQTDFCVDSATRSAMHRGFNVTLATDAHSTWDNGVLTAEQIIA
                     HHNKVLPSLPGPGTDISGKESAEIEFAPAGT"
     gene            4060..5568
                     /gene="gltX"
                     /locus_tag="VHR64_11410"
     CDS             4060..5568
                     /gene="gltX"
                     /locus_tag="VHR64_11410"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX3303481.1"
                     /translation="MNTSTPVRVRFAPSPTGSLHVGGGRTALFNYLFAYGQAKREGRD
                     GTFILRIEDTDQTRLVEGAIAEIQEVLHWFGLEWGEGPDKGGPHGPYIQSERIAMYQE
                     AAQRLIDEGKAYRCFCTRERLQMVREDQQKHGLPPGYDRYCRNLPQAEVQHALDQGMP
                     FTIRFAMPREGETVVNDVIRGEMVFQNELMEDLILLKSDGFPTYHLGNVVDDHDMEIT
                     HIMRGDEWIATAPIHVQLYKAFGWVVPQIAHMPLILAPGGGKLSKRHGSTAMEEFRSQ
                     GYLPEALMNYLALLGWSLDATTEIFSKEDLLKHFTLERVSPSPATFDYAKLLWFNQHY
                     INHILTVEDLVQRVVPFLVEAGLIEPEWANPDNPKYPLLHEATRLLKDRIKTLAEAPD
                     LMSYFLVEELPPYDATLLVPKKTEPGQVRTALLAVAELLESTDLDVLEGTEAALRGLA
                     EQQGLKAGQLFMPIRVAVTGRTESPGLFETLRGIGIERVRRRIGNAIALLNE"
     gene            5581..>6118
                     /locus_tag="VHR64_11415"
     CDS             5581..>6118
                     /locus_tag="VHR64_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX3303482.1"
                     /translation="MRRRDLLIGTVTFPLLRVVGVSAQASATPVPEDKWAHPEWFADP
                     NWIADHLDESAVRIIALTPAEDFAKGHIPGAAQVDWPDLNLTDSADATVEKWRGDIEK
                     RLTNLGVVPDSTVVIYDGGTFYGARLWWILDQLSHKDKRIIGGGLGAWTKSGGKIETG
                     AANPTSASQPYHGTPNEAT"
BASE COUNT         1100 a         1999 c         1932 g         1087 t
ORIGIN      
        1 cgatccgatt gctcgagtgg acggcaatgg cacgcatgcc aaccggattc ctttcgatac
       61 cggccgcgag atgccaatcg ccgtgctctg gatccggccc gaacagcagg cgaataccgg
      121 cctcgcggcc gacctcggct gcgagctcta cgatgccggg ctcattacgg gactggtcaa
      181 ggtcgagggc cctgggcaga cgacgatccc gggcgtcttt gccgccggcg acatcgccag
      241 cccgatgcac caggtctccc tggctgtcgc ctcgggaacg gcggccgccc tcggcgcaac
      301 ccgcctcttg atccagatgg acaacgcgct cgagtaaatc cgcccggttc tcgctgcact
      361 tccgctatcc tgtgccgatt acacgtggct ggaacgaggg ctggagtcat catgcggcaa
      421 ttcgaggggc ggggcgcgga catcctggcg gagtgtctga tcgaggccgg gattgatctc
      481 atcttcgggg tcccgggcga caccggcgtg gtcttctacg acgcgctcta ccaacggacc
      541 gaccgcatcc gccacatcct cacccgtgac gagcgccacg ctggtttcat ggcggacggc
      601 tacgcccgct ggacgaatcg ggccggctgc tgcgaggtca gctccggcgg cggggtcgat
      661 tttctggtca gcggcctggg cgaggcctat gccgcctctg tcccgatcct cgccatcacg
      721 tcggatatcc accgtcgcag ccgcgggacc agcgcgatca ccgagatcga ccaggagcag
      781 ctcttttcgg cggtcaccaa gctggcaatg acgatcgaga ccgccgcaca gatcccggac
      841 atcatccgtg tcctgctcca ggctgccacg accggccgcc cgggcccggt ggtcgcgatc
      901 ttccccgagg atgtcttcga cgagcacgca tcggtcacga tccccgagat gagcgcgacg
      961 gtcccgctcg atcgcgagcc ggcttcagcc cgcgcgacgc acaaggcagc ggagttgttg
     1021 gcgggagcgg aacgtcctgc aatcgtcgcc ggcggtggcg tccatctctc gtcggcctgg
     1081 ccggagttgc gagcgctggc cgagaagtcc ggcatcccgg tcgcgaccac gatccacggc
     1141 aagggcgcga tcgctgagac gcatccactt agtctcgggg tggtcggggc gaatggcgcg
     1201 cgacccttcg cgaacgcata tctctccgaa gccgatgcgg tcctgttcgt cggcacccgc
     1261 gccaactcga ccgacaccaa cggcttcacc tcgccgccgc gcgaagggcc gcaaatcatc
     1321 cagatcgaca tcgacaccaa ccgggccggc gtcaactttc cacagagcca cgatctcgtc
     1381 ggcgacgcca agacgacgtt ggccgcgatc accgaggtaa tcccggagaa cccgcagcgg
     1441 gccgagtcgc tcgcagcaca gatcggcgag tggcggactg cctggcgtct ggcggaggtc
     1501 gagccgcgcc gattgctcga ggctgacctg ctcgacccgc gcgacgtcgt gatcacattg
     1561 caggaggtgc taccggcgga cgtggtcatc ctggctgagc cgggtacgcc gacgccgaac
     1621 gtctccgcct attgggagtg ccccgagcca ggccggaccg tgatcgatcc gcgcgggcac
     1681 gggccgatgg gctacgtgat cccggccgcg atgggcgttt cgatgtccga tccggagcgg
     1741 ccgatcgtgg gactttgcgg tgacggcagc ttcgcgatgg cctgcggcga gctggagacg
     1801 gcgaaccggc tgcagttgcc ggtgctttac gtccagttca ctaacaactc gctgggctgg
     1861 atcaagatgc tccagcacct ctacctcggc cagcgctact tcagcgtcga tccgggcccg
     1921 atgaattacg tcggcgtcgc gcaggcgatg ggtctggagg caatccgggt caacgacctc
     1981 ggccagcttc gggaagccgt ccgcgagtgg ctaactcacc gccgcccaac tttcatcgac
     2041 gtcccggtcc ccgacgagat ttcgctgacg ccgcccgtcg caccctggca agccgcatta
     2101 gagggagcca ccggccgccc ggtgtactaa ctcatgccga cgatctgtat cgacccttcg
     2161 cgggcaacga cagggaaatg acgatgattg tacgtaaaga cacgacctcc gcgacgctca
     2221 gcgcacgcga ccacagcaga atctgcaaac ccaagagatg gatcgacgaa gcgagcggct
     2281 aacgcgcgga tacgtggaat gtcccgatcg ccgcagtcga gaaggtacac gcctcgatct
     2341 acgtctcgaa gaaactggtc cagggccgct tggccctgtc gtgtttcaat gaggtacgtc
     2401 agttcgggga ggatggggaa cggaataatc agtggccctc tctcattttc gatcagtgcc
     2461 ttcgcctcta ggtgatgtga atctgatcga tttagatgcg agtagattgc cgacgtgtca
     2521 agcgttatca cacgggattc cagttctccc tcagccattc gtccaggttc gtcgaatcga
     2581 cctccgtatc gtcgaagatc ccaactgctt ccggtcgtgg gcgtgagttt tctgtgacat
     2641 accgaccaat agcttcacga atgaccgcag ccttcggacg atcctcacgt ctggcaatgg
     2701 cctcgagggc cgccatctct tcttccggga tgtgaatagt cgtcttgatc atgtggcggt
     2761 tttaccacat gccacatgtg gctccacgaa cccatggggt aacagccccg aattcaatct
     2821 cctgagtcga tttgatgcag actcgttgtc cggggccgga catattcggc agcgtggcgt
     2881 tgacgtgggc gatgatctgc tcggcggtca ggatgccgtt gtcacaggtg gtgtgcgcgt
     2941 cggcagcgag cgttaccgcg aagccgcggt tcatggcgcc ccgcgttgcc gcatccacgc
     3001 agaagtcgga ctggatgccg caggtcacga gtcgctggat ccccgcatcg cgcaacacct
     3061 gctcaaggtt cgtcccggtg aacgcgtcgc agatccgctt gacgatgacc gtctcgtccg
     3121 gcttcggcgc gatcaccacc cggatcgcga agcccggctg acccggctgc aacgccgggt
     3181 cggtctgatc gtgatgctgg atgtagatca cccgcgcgcc ggccgtccgc gctttggcaa
     3241 gcagcgcggc gatgttggag acggtctcgt cagcccggaa taccggcggt tgttgctccg
     3301 ggccgaacat cgcctcctgc acgtcgatga ccaggagcgc cgctggcaga gacgattcct
     3361 ctacggacat cggcgggaca gcctacgtgc cggccggggc gaactcgatc tcggctgatt
     3421 ccttgccgga gatgtcggta cctggacccg gcaggctcgg caggaccttg ttgtggtggg
     3481 cgatgatctg ctccgctgtc agcacgccgt tgtcccacgt ggaatgcgcg tcggtggcca
     3541 acgtgacgtt gaagccgcga tgcatggccg agcgggtggc cgaatcgacg cagaaatcgg
     3601 tctgcattcc gcaggtcacc aggtgcgtga cccctgcctc acgcaggatc ggctccaggt
     3661 cggtcgcgca gaatgagtcg caagcggtct tgtcgatcac ggtctcgccc tcgagtggcg
     3721 cgattgccgg gtggacgtcg aaggcgggat gaccaggcga catcaggggg tacttcgatt
     3781 ccacgtgacg gacgaagatc accttcgacc cggccgcgcg ggcgttctcg agcatggtgc
     3841 cgatgttcgc cagcacctcg tccttgcgga agggcgacgg gtcatccggg tagtcatcca
     3901 tcatcgcgcc ctggacgtcg atcactacca atgcggtcgg cttgctctgt tcgctcatca
     3961 atctgactcc cgctcaaatg acgccttcta tacttctccg cggtgcgcgc ccgggcctcg
     4021 agatcgcccg tcggtgatgc gcatcggagg agaactctca tgaatactag cacacctgtt
     4081 cgtgtccgct ttgcgcccag cccaaccggc tcgctccacg tcggcggcgg ccgcaccgcc
     4141 ctcttcaatt acctctttgc ctacggtcag gcgaagcgcg aaggacgcga cggcaccttc
     4201 atcctccgta tcgaagacac cgaccagact cgcctcgtcg agggcgcgat cgccgagatt
     4261 caggaggtcc tgcactggtt cgggctcgaa tggggcgagg gaccggataa aggcggtccc
     4321 cacggcccgt acatccagag cgagcgaatc gcgatgtacc aggaggcggc ccagcggctg
     4381 attgacgagg gcaaggcgta ccgctgcttc tgcacgcgcg agcggctcca gatggttcgc
     4441 gaggatcagc agaagcatgg tctcccgccc ggctatgacc gctactgccg taacctgccg
     4501 caggctgagg tccagcacgc gctcgaccag ggcatgccat tcacgatccg ctttgccatg
     4561 ccgcgcgagg gcgagacggt cgtcaacgac gtgattcgcg gcgagatggt cttccagaac
     4621 gagctcatgg aggacctgat cctgctcaag tcggacggct tcccgaccta tcatctcggg
     4681 aatgtcgtcg acgaccacga tatggagatc acccacatca tgcgcggcga cgagtggatc
     4741 gccacggcgc cgatccatgt ccagctctac aaggcgttcg gctgggttgt cccgcaaatc
     4801 gcgcacatgc cgctgatcct cgctccgggc ggtggcaagc tctcgaaacg gcacggctcc
     4861 actgcgatgg aggagttccg cagccagggc tacctgcccg aggccctgat gaactacctg
     4921 gccttgctcg gctggagcct cgatgcgacg accgagatct tctcgaagga ggatctactc
     4981 aagcacttca cgctcgagcg agtcagccca tcgccagcga cgttcgacta cgccaagctg
     5041 ctctggttca accagcacta catcaaccac atcctgaccg tggaggacct ggtccagcgg
     5101 gtcgtgccgt tcctggtcga ggccggcctg atcgagccgg agtgggcgaa cccggacaac
     5161 ccgaagtacc cgttgctcca cgaggcgacg cggctgctca aggatcgaat caagaccctg
     5221 gccgaggcgc cggacctgat gagctacttt ctggtcgaag agctgccgcc ctacgacgcg
     5281 accctgctcg tcccgaagaa gactgagccg ggacaggttc gcactgcgct cctggccgtt
     5341 gccgagctgc ttgagtcgac cgacctggac gtactcgaag gcaccgaggc ggccctgcgc
     5401 ggtctggccg agcagcaagg gctcaaggct gggcagctct tcatgccgat ccgcgtagcc
     5461 gtgaccgggc gaacagaatc ccccggcctc ttcgagacgt tgagggggat agggatcgag
     5521 cgcgtccgtc gccgcatcgg caatgcgatt gcactcctca acgagtaatc ggagggcgtc
     5581 atgcgacgtc gagatctgct gattggcact gtcacctttc cgctactccg ggtagtcggg
     5641 gtgtcggcac aggcaagcgc aacgccggtt ccggaggaca agtgggcaca cccggagtgg
     5701 ttcgctgatc cgaactggat cgccgatcat ctcgatgagt cagcggtccg gatcatcgcc
     5761 ctgaccccgg cggaggactt cgcaaagggc cacatcccgg gtgcggccca ggtcgactgg
     5821 ccggatctca acctgaccga ctcggccgat gccacggtcg agaaatggcg cggagacatc
     5881 gagaagcgtt tgaccaacct cggcgtggtg cctgacagca cggtcgtcat ctacgacggt
     5941 ggcaccttct acggcgcgcg actctggtgg atcctcgacc agctcagcca caaggacaag
     6001 cgcatcatcg gcggcggact cggtgcctgg accaaatccg gtggcaagat cgagaccggc
     6061 gcggccaacc cgacctcggc atcccagccg tatcacggta cgccgaacga ggcgaccc
//
LOCUS       DATAOZ010000447        16423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4387741, whole genome shotgun sequence.
ACCESSION   DATAOZ010000447 DATAOZ010000000
VERSION     DATAOZ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 16423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16423
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4387741"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VHR64_11420"
     CDS             complement(<1..385)
                     /locus_tag="VHR64_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303483.1"
                     /translation="MISFRHIPIRLRLSLWYTILTALVLVIFSFALYIGLERQLSTTL
                     DQDLRNQAALASSSITYAGGNPIFASSFNEQLTDVVLLWVVRFDTQQLVKVRGQTLEI
                     EPLMTEDLSRATFGLSTYKTVKVSNQ"
     gene            complement(382..1056)
                     /locus_tag="VHR64_11425"
     CDS             complement(382..1056)
                     /locus_tag="VHR64_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3303484.1"
                     /translation="MRILVVEDEIRLADLIKRGLSHEGHAVDLAETGEEALDWSDAAN
                     HDAIVLDIMLPGISGLEVCRTLRKRQIQTPILLLTARDAVPDRVAGLDAGADDYLIKP
                     FAFPELLARLRALARRPAQTHQTRLATGDLALDPASRQVWRDGREVQLQNKEFRILEY
                     LMRNPNRVLSRDQIAEYAWDYDFPAVTNVIDVHIKSLRRKLDDPYPGQLIQTVRGVGY
                     RLTIEA"
     gene            1339..1998
                     /locus_tag="VHR64_11430"
     CDS             1339..1998
                     /locus_tag="VHR64_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3303485.1"
                     /translation="MRWVLVAGMLAAFVALAIGAHRVVAFTIDVRFTRWVQDHDSTLL
                     DWLTTLTNRVMGGTLLSLVGVAIAIALLARRHPLDAAVVATATAVRVTNSGFKEIVAS
                     PRPTPDLVRVTDHANGYGYPSGHAASATLVVGAVAWVVARHVRSRAGRIAVWLVAAVL
                     IILTGIGRVRVGVHWPTDVLGGWLWAGAALLVITGLAGQQARRGEQARRRGSEQLDGG
                     P"
     gene            2002..2646
                     /locus_tag="VHR64_11435"
     CDS             2002..2646
                     /locus_tag="VHR64_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3303486.1"
                     /translation="MVRPGLAVGMLAAFVALAWAAHGDSVLQGDVRLTRWAKGRSWPL
                     LDELTDAANWSMRSVPLIVAGLFLVGVLAWRRMWPEAAIMTLALILMHLSYPLKEIVA
                     SPRPSADLVEETEGGGGYGFPGGRAGNAVLVIGACAWIAARHGESRWARTAIWEGAGL
                     WIFVVGLARIMVGAHWPSDILGAWLWTIPVLIAIASAMDKWRTLSRSGEAPYSP"
     gene            3183..4151
                     /locus_tag="VHR64_11440"
     CDS             3183..4151
                     /locus_tag="VHR64_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX3303487.1"
                     /translation="MTLSWPTPTLADIRAAQTRIAPIAVRTPIIKLDWDEAPGDLYLK
                     LELLQPIRAFKIRGAYNAMAALDRSEIEQGVWTASAGNHAQAVAMSARALGVPCTVYV
                     PDTAPRTKINNAIRYGARVVELPVMEWVDIFRNRGYEHATGFFVHPYSDPNVIAGQGV
                     CGLEILEEMPDVETIIAPWGGGGLTSGIASATAAMSPQVRVIASEIETGAPLGPSLEA
                     GHPVEVPYRPSFADGIGTPFVNEEMYELATRLGIRAIGTTLEQTADAMRIMVSRAAIT
                     PEGAAATPLAAAISGGAGTGKTVLVISGGNIDSATLQTILAGGTPE"
     gene            4472..4981
                     /gene="dps"
                     /locus_tag="VHR64_11445"
                     /gene_synonym="pexB"
     CDS             4472..4981
                     /gene="dps"
                     /locus_tag="VHR64_11445"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEX3303488.1"
                     /translation="MATATKAMTTKMFKTSIDIPEATRSKVVEYLNQNLADAFDFLSQ
                     SKQAHWNVKGSDFYQLHKLFDEIAGEAIEWVDMVAERVTALGGYATGTARMAAKSSQL
                     PEFPTDITDGMDFVRALVERLAAYSNSCREGIDQTEKWGDVDSSDLLTEISRCADKYL
                     YFLEAHLRS"
     gene            complement(5335..5589)
                     /locus_tag="VHR64_11450"
     CDS             complement(5335..5589)
                     /locus_tag="VHR64_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303489.1"
                     /translation="MFEDRGNWIGIALLVLCGLSAGTMLGYIRAGEIARFSGPQWLGA
                     TVIVAAIGLTIYMTIQAFRGRHLGDDLRGPRRRWWRRNKG"
     gene            complement(5642..6295)
                     /locus_tag="VHR64_11455"
     CDS             complement(5642..6295)
                     /locus_tag="VHR64_11455"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX3303490.1"
                     /translation="MITLQSVNVGMPEVIGFRRGGPVLSGFRKRPIEFTYVDVGETNI
                     AGDAQADLRAHGGHHTAVYAYCADHFAWWTKQVKLEHPVGPGTFGENLTISGIDEAAA
                     CIGDVWRWGTVVLQVSQPRYPCYKMAMATGRPAIVKRFLESNRSGWYLRVLEEGEGPV
                     GGEICLLDRDPAGLTVRDAARASWQSTEPERLIEIAAHPALAPSWANLLRHNAISPV"
     gene            complement(6292..7077)
                     /locus_tag="VHR64_11460"
     CDS             complement(6292..7077)
                     /locus_tag="VHR64_11460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3303491.1"
                     /translation="MNIEPGRIRLVSFDLYDTLIELEPKRWERLSAVLAARGITHDPA
                     ILKEKDMVAEDYWTEINAIRPIRERDEDVQDEIRVEYIRRWLQASGVVVDDAIATEIR
                     HAYRAEYETHARQSIPGMVDGYRVFPDVNRTMRRLHEADVLCAVISNADDDVTEFCTR
                     LQFAHEMDLIVTSALVGYEKPDVRTFQAALEPFDISGPEALHVGDQPRSDVAGALATG
                     MRAALIDRYRRHRQENHSVPIFHNLDNLVDEVLAINEAAGVMA"
     gene            complement(7111..8064)
                     /locus_tag="VHR64_11465"
     CDS             complement(7111..8064)
                     /locus_tag="VHR64_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX3303492.1"
                     /translation="MIEYADIVSAAERLNGIAHHTPAVTSRSVDERTGATVFLKAENL
                     QRVGAFKFRGAYNKISRLSDDELRRGVVTVSSGNHAQAVALAATLMGTTAKILMPQDA
                     PAIKVAGVKGFGGEVVPFDRYAQDREALLHELADREGRVIVHPYDDPLVMAGQGTCVK
                     ELIEDVGLLTHLFVCVGGGGLISGCSTAAKALVPDCCVIGVEPETGNDTQKSLERGER
                     VSIPVPETIADGQQTTAPGKLTFPVIQQRVDQIVTVTDAEIVAAMRFAVERLKLVLEP
                     SGACAFAAVLTGKVDVAGGRVGVTLSGGNVDLARFCSLAAS"
     gene            8125..8607
                     /locus_tag="VHR64_11470"
     CDS             8125..8607
                     /locus_tag="VHR64_11470"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEX3303493.1"
                     /translation="MERTDLLAAVDDGWNEWQTMLATLTPEQMVESALPGGWSVKDAI
                     AHIAFYEWWVGEFIRTRSWPPAPHPSLDTWDMDARNDAFFELNRDRSLDDVLAESHRV
                     HQVLVDAVSALTDAEYHDPNLLGTPPDDEWRVEKMVDGNTFKHYSEHAAVIRERFGSE
                     "
     gene            complement(8666..9979)
                     /locus_tag="VHR64_11475"
     CDS             complement(8666..9979)
                     /locus_tag="VHR64_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3303494.1"
                     /translation="MTTRVNRRTLLKGAAGTAAAAAVSPVFHAPNVIAQSATEITYWG
                     SFSPGSVNGDAEAGMVDRFNKAQSDVVVKYEFQGSYEETGQKLTAALAAKQTPDVCLL
                     SDVWWFKFYLNQALAPLNDLMSANNVDPADYVDSLINEGNRDGVQYWMPMARSTPLFY
                     YNKAALQEAGLSEAPKTWDELAAAAPKLVKKDGDTLTQAAYAHRTAANYIAWVFQGLV
                     WAYGGQYSDADFNMLINQGGAVDAGNQYLASVTDGWASTPSDVDADFLNGLTATMIGS
                     TGGLTRFIKEATFDWGTAFLPEGPAGFGCPTGGSGLGVMAASEKQDAAFKFVAFTSTP
                     ENTAWWSLQTGYMPVRKSAVDLPELKDFYDKNPQAKVAVDQLAKTKPQDSARVFVPNG
                     DQIIGKGLEQITVNKEEVQSVFDGVNQTLTDEAQPVKEALQDLQS"
     gene            complement(10104..11000)
                     /locus_tag="VHR64_11480"
     CDS             complement(10104..11000)
                     /locus_tag="VHR64_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3303495.1"
                     /translation="MAVEAISIGSQERTWLSQRTRVRAWRLLGYLALVVLVIAIGAPV
                     YWMLTASVKSNAEIYTFPPQWIPSNPRWANFQDAWNAAPFDRFYIVSIVTTLFGTGLE
                     LVNATLTAYALAFLRFPFKNLVFVIILATLMVPTQLTIIPNYLTISDVPLIHSNWINT
                     YQGIILPGAAVAFGTFMLRQGFLGLPREVLDAAKVDGAGHLRTLWDIAIPMAKPIIVT
                     FGLISMVAKWNDYLWPLIVTNEERMRPLAVGVSYLNDPDGNTQWGMVMAAVFFVIAPL
                     IVVYIFAQRYIIEGIAAGATKG"
     gene            complement(10990..11895)
                     /locus_tag="VHR64_11485"
     CDS             complement(10990..11895)
                     /locus_tag="VHR64_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3303496.1"
                     /translation="MADSIRAVKRKASSEWLLFLLFVGPNLILFGIFTYWPLIYSFYL
                     STVRWDMLAPRKRPVGLGNYEYLLHNDTFHKVMWNSLFFTVGAVGGSMLLGLLAALLL
                     NQKLRGRNTARAIVFMPTLLSGAALGIVWVYIFDPRYGLMRTVLSLLGIGSPNWLRDT
                     SWALPAIIIVYIWKNLGFAAIIYLAGLQGIPKDLYEAARVDGAGPFWRFRSVTMPMLS
                     PITFFLVITSILNSFQAFDIIRVMTQGGPIDATNTLIYYVYDEGFVAFNAGRASAAAV
                     VLFVIMLAITLVQLRYQERRVHYGG"
     gene            complement(12147..13715)
                     /locus_tag="VHR64_11490"
     CDS             complement(12147..13715)
                     /locus_tag="VHR64_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX3303497.1"
                     /translation="MREVELVLILLAAVAVLVSIAGRLSIPYPILLVLGGLVLSFVPG
                     LPVIVLDPHVVFVLFLPPLLYWDAASTSWRDFRADIRTITLLAIGLVVATTLCVGILA
                     HSVLHLSWTAAFTLGAVVSSTDPVAASAVMKRMGVPPKVIAIVQDESLVNDATALVVF
                     AAAIEALKTGSFSLGHASLEFVFVAVGGALVGLASGWVLYLLQHQIDEARVSGVLRLM
                     TPFVVYILAESIHVSGILAVVVTGLYSGRKAPTDLTSEERLGTAVIWQMATFIINGLV
                     FILIGLQFSSILDAIDERSTSSLIKDGLLISLAVIGVRMVWVYLGAGAIRLLPQRLTG
                     REEELDVREMTIVGWCGMRGVVALATALSLPWVTNTGMFTERPLIQFLTFVVIIVTLV
                     GQGLTLPALITALGVTRGDRSLREEQRARGAMTRAALKQLDRLSRDFAGNEVYGTLRR
                     HYQNLLTRTSASSAPGDESWVQSVRDLRRELLDVEHQTLVQLRDQDVISDAVLHSLQR
                     ELDIEAMHLEARVI"
     gene            complement(13803..14825)
                     /locus_tag="VHR64_11495"
     CDS             complement(13803..14825)
                     /locus_tag="VHR64_11495"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3303498.1"
                     /translation="MIDASSPTIANERERREAVTQSRVIVALFVIYVVAGTALSYIRG
                     GSFLTPDRIAVLLLAGAIVLGQGKAFVRDWGPFLLLLFGYELMRGVADNMTNLGAYNA
                     NDHGRILVQSLIDGDKALFFGHIPTLWLQDRLYTPGVTHWYDTGAALIYMLHFVLPLA
                     FGFALWLKDRTMFRRFVVMLLAMSYGAFVFFLLVPAAPPWLAADWGYIDGVDRPSYQA
                     YKAFLPHRWENYDTFRLWTRASPNPVAALPSLHAAFPWLVLLIACTTFRWWGLLLVPY
                     NVALWFSVVYLSQHWAVDVLAGIVWATAIWAIFMWIYSWRHRARSDLPIAASFLTPIP
                     GDDSPP"
     gene            complement(14822..>16423)
                     /locus_tag="VHR64_11500"
     CDS             complement(14822..>16423)
                     /locus_tag="VHR64_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303499.1"
                     /translation="LVSYTSFLQRRSWGFLVVSGIAAGLALLTRSVNGVLLPLMVVLT
                     LFDIYRRAASEQIPIRGQVRFHFVRMIVWGLIALATVFALWPALWVAPIDTIDQLVRG
                     GSTLASAPHTRQLLFRGQVTGSDPGWSYYLVVLAYRLSPLTAVALVLSTFGLLAPRVG
                     AAEPGRRLCFNLAVFAAAYFVILSFAAKKLDRYLLPTLAALDLIAVIGTIALVRYLVS
                     QRAGISRYVATGIAVAAFLVLLGGQAVAARESAPYYITYVSPLMGGQSAARRSISLEW
                     GEGGKAVAETILQHPEITPGTIAGGAWPRAIDYYLPFPLLSANHDLNPAGAAWFMQSQ
                     YVVVTEPEIQRQFYALSMIVWFDSQSPVAVIQDDGRVYARIYDLSGLRVPEPYYQPDA
                     PIFTWRGQATLVGSVYREQIEVGSDLRLRLFFETGGQPFYYRIDARVVDSAGNVVGEI
                     RKGLRAESPVPSQMRSIVNIDLPDDLPPGTYQIQIRMRDGETHETVQAVRAAIDQNAR
                     SPVTIGSFEITTDIPENGTPVDAES"
BASE COUNT         3172 a         5070 c         5328 g         2853 t
ORIGIN      
        1 tttggttact gaccttgact gtcttgtagg tcgagaggcc aaacgtggca cgcgacagat
       61 cttctgtcat gagcggctca atctctaatg tttggccgcg tacctttact agctgctgag
      121 tgtcgaatct gaccacccac aacagcacca cgtcggtaag ttgctcattg aatgaggaag
      181 cgaaaatcgg attgccacca gcgtatgtga tcgagctgga cgccaacgcc gcctggttgc
      241 gcagatcctg atcgagggtc gtggagagct ggcgctccag gccgatgtag agggcaaacg
      301 aaaaaatcac caggaccagc gcggtcagga tcgtgtacca aagggacagg cgcagccgga
      361 tcgggatatg ccgaaagctg atcatgcctc aatcgtaaga cggtacccaa ctccgcgtac
      421 cgtctgaatg agttgaccgg gatacgggtc gtccagcttg cggcgcagcg atttgatatg
      481 gacgtcgatc acgttggtca cggccgggaa gtcgtagtcc caggcgtact cggcgatctg
      541 gtctcgtgac agcacgcggt tcggattgcg catcaagtac tcgaggatcc ggaactcctt
      601 gttctgaagc tggacctcgc gaccatcgcg ccagacctgc cgggaggccg gatcgagcgc
      661 gaggtcgccg gtcgctagcc gggtctggtg cgtttgggct ggacgtcggg ccaacgcgcg
      721 caaccgggcc agcagctccg gaaaggcgaa aggcttgatc aggtaatcgt ccgcgccggc
      781 gtcgaggccg gccactcgat cgggcactgc gtcgcgcgcg gtcaggagca ggatcggcgt
      841 ctggatttgg cgcttgcgca gggtgcgaca gacttcgagg cccgagatgc cgggcagcat
      901 gatgtcgagg acgatcgcgt cgtgattggc ggcatccgac caatcgagcg cctcttcgcc
      961 ggtctcggca agatcgactg catggccttc gtggctgaga ccgcgcttga tcagatcggc
     1021 cagtcgaatt tcgtcttcaa cgacgaggat gcgcatgaaa cctgcctgcg tttgccgggc
     1081 gcgttgatcc ctgcgcccca attgtcgcct gaatcgatgg tgaaagtcag tgatgaaacc
     1141 tacgtgaact aagtcgagaa cgggtggatg gtggttggtc gttggtggat ggaacaggga
     1201 acaggggaaa gtgggctggg atcggatacg ctcatcccaa aacccaaaac ccaaaaccca
     1261 aaaccaaaga cgagagacga atgaccgaga cctccgtatg cttcaatctt cgtcgggagg
     1321 tgttgcatct tggatcgggt gcggtgggtg ctggtcgccg ggatgctggc tgcgttcgtg
     1381 gcacttgcta tcggtgcgca tcgggttgtc gcgtttacga tcgatgtccg attcacacgc
     1441 tgggtgcagg atcacgattc gaccctgctg gactggctga cgaccctgac aaaccgggtg
     1501 atggggggaa cactgctgtc tttggtcggc gtcgcgattg cgattgcgct gctggcccgg
     1561 cgacacccgc tcgatgcggc ggtggtggcg acggcaactg ctgtccgggt gaccaattct
     1621 ggattcaagg agatcgtcgc ctcgccgcga ccgacccctg atctggtccg cgtgaccgac
     1681 catgccaatg gatacgggta tcccagcggt catgcggcca gcgcgacact ggtcgtgggt
     1741 gcggtggcgt gggtcgtggc ccgtcacgtt cggtcccggg ccggacggat tgcggtctgg
     1801 ctggtcgcgg cagtgttaat aatcctgacc gggatcggta gggtgcgcgt gggtgtccac
     1861 tggccgaccg acgtgctcgg cggctggctt tgggcgggcg cggcgctact ggtgatcacg
     1921 ggcttggcgg gccagcaggc aagacgaggc gagcaggcga ggaggcgagg aagcgagcag
     1981 ttggatgggg gaccctgagt cttggttcga cctggcctcg ccgtcggcat gctcgcggcg
     2041 tttgtggcgt tagcgtgggc agcgcatggc gattcggtgc tgcagggcga tgtgcggttg
     2101 acgcgctggg ccaaggggcg aagttggccg ttgctcgacg agttgactga tgccgccaac
     2161 tggtctatgc ggagtgttcc cttgatcgtc gccggcttgt ttctcgtcgg cgtgcttgcc
     2221 tggcgtcgaa tgtggcctga ggcggcgatc atgacactcg ccctgatcct catgcacctg
     2281 agttatccgc tcaaggagat cgttgcttcg ccgcgcccct ccgcggatct cgtggaggag
     2341 accgaaggtg gaggcggata cgggttcccg ggcggtcggg ctggcaatgc ggtgctggtg
     2401 attggggcgt gcgcgtggat tgccgcccga cacggcgaga gccgatgggc gcggaccgca
     2461 atctgggaag gcgccgggct gtggatcttc gtggtcgggc tggcccggat catggtcggc
     2521 gcgcactggc caagcgacat cctcggcgct tggctctgga ccatcccggt attgattgca
     2581 atcgcgtcag caatggacaa atggagaaca ttaagccgta gtggcgaggc tccgtactcg
     2641 ccttgaccca cccagtcatc gggttcacgg tgccggcgga gtctccgtca tccgcgccac
     2701 cggggattga aatcccggtc tcagcatcgg aagtcggttg acaccgattg gcgcatttcg
     2761 ggttgattgg caagaggtta ggtcgaattg ctatgcgact ctccaagcgg ccatgccccg
     2821 ggcgggcaga tcgccgtctg ccccttcaat tgctcgcctc ccgactgctc gccttcaaaa
     2881 agagaatcgg cgcaatcgaa ttgcttcgac cgcgccgatc gggttgggga ggtggcggta
     2941 ggagggttcc tacccggagc gctggttgct cgctccgttg gtgacactat cagggaccaa
     3001 tatgaactgg cgatgaaccg gggaaaagtg ggtggagggt tgagagttga gggttgaggg
     3061 aacggtggac aggggacagt gaggggcggc cggatacgct ctttcccaaa acccaaaacc
     3121 caaatttgcg tccgtcctgc gacacttccc gcacgatccg ttatcacgtt caaggaggaa
     3181 ccgtgacact ctcctggccc acgccgacgc tggccgatat ccgggccgcg cagactcgca
     3241 tcgcccccat tgccgtccgc acgccgatca tcaagctcga ctgggacgag gcgccgggcg
     3301 atctctacct caagctcgag ctgcttcagc cgatccgcgc cttcaagatt cgcggcgcct
     3361 acaacgcgat ggccgcgctc gaccggtcgg agatcgagca gggcgtttgg accgcctccg
     3421 ccggcaatca cgcccaggcg gtcgcgatgt ccgcccgcgc gctcggggtt ccctgcacgg
     3481 tctacgtgcc cgataccgcc ccgcgcacca agatcaacaa tgccatccgg tacggcgcgc
     3541 gcgtcgtgga gctcccggtc atggagtggg tcgacatctt ccgcaatcgc ggatacgagc
     3601 atgccaccgg tttcttcgtg catccttaca gtgacccgaa cgtgatcgcg gggcagggcg
     3661 tttgcgggct cgagattctc gaagagatgc ccgatgtcga gacgatcatc gcgccatggg
     3721 gcggcggcgg gctcacctcc gggatcgcat cggccaccgc agccatgtcg ccgcaggtcc
     3781 gggtcattgc cagcgagatc gagaccgggg cgccgctcgg accatctctc gaggccggcc
     3841 atccggtcga ggtgccgtat cggcccagct tcgccgatgg catcggcacc ccgttcgtca
     3901 acgaggagat gtacgagctg gcgactcggc tcggaatccg ggcaatcgga acgacgctcg
     3961 agcagacggc cgacgcgatg cggatcatgg tcagccgagc cgcgatcaca ccggagggtg
     4021 ctgccgctac gccattggcc gcagccatta gcggcggcgc cggcacgggc aagacggtcc
     4081 tcgtcatctc cggcgggaac atcgattccg cgaccctgca aaccatcctc gccggcggca
     4141 cgcctgagta gccaccatcc agcaacaaga cataacacag tagaccgggt gccaatcgac
     4201 acccggtcta ctgtgagggg agaggagtgg gaacgccgcg gctgcaacgg cgtcatggct
     4261 ggagattcgg atctggagcc tcggctcccg gttggtgcgg cgcgtcctcc gtcagtcaat
     4321 aaccgaacac ctcatggccc tatggtgccg ccggctgaga ggtccggcat cggtaggatc
     4381 acgtaaatgc tcggggaggc ttctttgcta gggtaggcag ggtaagaggc gtgcagcacg
     4441 cacgcgacgt tcgtcaaagg agacacgaac aatggcaact gccaccaagg cgatgaccac
     4501 caagatgttc aagacgagca tcgatatccc ggaggccacg cggtccaaag tcgtcgagta
     4561 cctgaaccag aatctggccg acgcattcga cttcctgagc cagtcgaagc aggcccattg
     4621 gaatgtgaag ggatccgatt tctaccagct ccacaaactc ttcgatgaga tcgccggcga
     4681 agcgatcgag tgggtcgata tggttgctga gcgggtgact gcactcggcg ggtacgccac
     4741 aggcacggcc cggatggccg ccaagagcag ccagctcccc gagtttccta ccgatatcac
     4801 cgacggcatg gacttcgtcc gggccctggt cgagcggctg gcggcctatt cgaactcgtg
     4861 ccgcgagggc atcgatcaga ccgagaagtg gggcgacgtc gattcatccg acctgctgac
     4921 cgagatcagc cgctgcgccg ataagtacct gtacttcctg gaggcgcacc tgcggtcgta
     4981 gggatctcag gggacagtag tcagaaaagg acgggcgcgg aattgcatcc gcgcccgtcc
     5041 ttcgtagtgg cgtggctccg tacccgcctg gagtcactcg accgtacctc gctatgcatc
     5101 gcaccgttta cgtagcgcgt taccacgccc cgctcagacc cgaggatggc tcattcgcta
     5161 ggaaaccaac tccatccgca tttcggcccc gtctaccgtg cgtggggatg aacgaagcgg
     5221 ggcatggtaa ccccgcccca catccgaatg tcgatatatg ccaaggggtc tccgggatgg
     5281 tccaggccgt acagagcccc gccactacgg tcgaggtgaa tctggtggcg agctctagcc
     5341 cttgttgcgg cgccaccagc ggcggcgtgg gccgcggagg tcgtcgccaa ggtgccggcc
     5401 gcggaacgcc tggatcgtca tgtagatggt cagcccgatg gccgccacga tcaccgtcgc
     5461 gccgagccac tgcggacccg agaagcgcgc gatctcgccg gctctgatat agccgagcat
     5521 cgtcccggcg gagagtccgc acaggaccag cagcgcgatt ccgatccagt tgccgcgatc
     5581 ctcgaacacg acgggtcact ccttcgatgc agcccgcgtt gatgatacgg cgtgcggcaa
     5641 atcagactgg gctgatagcg ttgtggcgca acaaattggc ccagctcggc gcgagcgccg
     5701 gatgcgcggc gatctcgatc aggcgctcgg gctcggttga ctgccaggat gcccgtgccg
     5761 catcccgcac ggtcagcccg gccggatcgc gatccagcag gcaaatctcg ccgccgaccg
     5821 gtccttcgcc ctcctcgagc acgcgcaggt accagccgct ccggttggat tcgaggaatc
     5881 tcttgacgat cgccggccgc ccggtggcca tcgccatctt gtagcaggga taacgcggct
     5941 ggctcacctg cagcacgacc gtgccccacc gccagacgtc gccgatgcag gcagccgctt
     6001 catcgatgcc ggagatggtc agattctcgc cgaaggtgcc ggggccgacc ggatgctcca
     6061 gtttgacttg cttcgtccac caggcgaagt gatcggcgca gtaggcatag accgctgtgt
     6121 ggtgaccacc atgcgcgcgc agatcggcct gggcatcgcc ggcgatgttg gtttcgccga
     6181 catcgacgta cgtgaactca atcggccgct tccggaagcc gctcaggacc ggtccgccgc
     6241 gccgaaaccc gatcacctcg ggcatcccta cgttcaccga ctgcagcgtg atcacgccat
     6301 caccccggcc gcttcgttga tcgcgaggac ctcgtcgacc aggttgtcga ggttgtggaa
     6361 aatcggcacg gaatggttct cctggcgatg ccggcggtat cggtcgatca gtgcggcacg
     6421 catcccggtc gcgagcgcac ctgccacgtc tgagcgcggt tgatcgccga catggagtgc
     6481 ttccggcccg gagatatcga acggttcgag cgccgcctgg aaggtgcgca cgtccggctt
     6541 ctcgtatccg accagcgccg acgtcacgat caagtccatc tcatgcgcga actggagccg
     6601 ggtgcagaac tcggtcacat cgtcgtcggc attcgagatg accgcgcaga ggacatcggc
     6661 ctcgtgcagg cggcgcatcg tccggttgac gtcggggaag acgcgatacc cgtcgaccat
     6721 cccgggaatc gactgccggg catgggtttc gtactcggcc cggtaggcgt gccggatctc
     6781 ggtcgcgatg gcgtcgtcca cgacaacacc ggacgcctga agccagcgcc ggatgtattc
     6841 gacgcggatc tcatcctgga catcttcgtc gcgctcgcgg atcggacgga tcgcgttgat
     6901 ctcggtccag tagtcctcgg cgaccatgtc tttttctttg aggatggctg gatcgtgcgt
     6961 gattccccgc gcggccagta ccgccgagag ccgctcccag cgtttaggtt cgagttcgat
     7021 cagcgtgtcg tagaggtcga acgagacgag tcggattcgg ccgggttcga tgttcatgcc
     7081 ggaatcatag ccattccgcg ccggggaggc tcacgaggcc gccagcgagc agaatcgggc
     7141 cagatcgaca ttgccgcccg agagcgtgac cccgactcgc ccgccggcga cgtcgacttt
     7201 gccagtcagc acggcggcaa aggcgcaggc gccgctcggc tcaagaacca gcttgagccg
     7261 ctcgacggcg aagcgcatag ccgcgacgat ctccgcgtcg gtgacggtca cgatctgatc
     7321 tacgcgctgc tgaatgaccg ggaaggtcag cttgccgggc gcggtggtct gctgcccatc
     7381 ggcaatcgtt tccggcaccg ggatcgagac ccgttcgccg cgttcgagcg acttctgcgt
     7441 gtcgtttccg gtctccggct cgacaccgat cacgcagcaa tcgggcacga gggccttggc
     7501 ggccgtcgag caaccggaaa tcagcccgcc gcctcctacg cagacgaaga ggtgggtcag
     7561 cagccctacg tcctcgatca gctccttaac gcaggtgccc tgaccggcca tgaccagcgg
     7621 gtcgtcgtac gggtgcacga tcacgcggcc ctcccgatcg gccagttcat ggagcagcgc
     7681 ctcccggtct tgggcatagc ggtcgaacgg caccacttcg ccgccgaacc ccttgacgcc
     7741 ggcgaccttg atcgccgggg cgtcctgcgg catcaggatc ttcgcggtcg tgcccatcaa
     7801 tgtggcagcg agcgcgaccg cctgggcgtg attgccggag gagacggtca cgacgccccg
     7861 gcgcaactcg tcatcggaga ggcgcgagat cttgttgtag gcgccgcgga acttgaacgc
     7921 gccaacccgc tgcaggttct ccgccttcag gaagaccgtt gctccggtcc gctcgtcgac
     7981 gctgcgggag gtgaccgccg gcgtatggtg ggcgatgccg ttgagccgct cagcggcgga
     8041 gacgatatcg gcgtactcga tcatgtgcta tcctcgctcg aacacccgtt ctacaccgac
     8101 tgatcctgcg gaggaagcat accgatggaa cgcaccgatt tgctggcggc cgtcgatgac
     8161 gggtggaacg agtggcagac catgctggca acgttgactc ccgagcagat ggtcgagtcg
     8221 gccttgccgg gtggatggtc ggtcaaggac gccattgcgc acatcgcctt ctacgagtgg
     8281 tgggtcggcg agttcatccg cacccgcagc tggccaccgg ctccgcaccc atcgctcgat
     8341 acctgggaca tggatgctcg aaacgatgcc ttcttcgagt tgaacaggga ccgctccctc
     8401 gacgatgtcc tcgcagagtc gcaccgggta catcaggttc tcgtcgatgc cgtttcggcc
     8461 ctgactgacg ctgaatacca cgacccgaat ctgctcggca cgccgcctga tgacgagtgg
     8521 cgcgtcgaga agatggtcga tggcaacacc ttcaagcact actcggagca cgccgccgtc
     8581 atccgagagc gattcggaag cgagtgatcc gggaatgtaa aacgccgccg gggatatctc
     8641 tccggcggcg ttcgtgcacg tccgattacg actgcagatc ctgaagcgcc tccttgaccg
     8701 gctgtgcctc gtcagtgagg gtctggttca cgccatcgaa gaccgactgg acctcttcct
     8761 tgttgaccgt gatctgctcg agacccttgc cgatgatctg gtcaccgttc gggacgaaga
     8821 cgcgggcgga atcctgcggc ttggtcttgg cgagctggtc gaccgcgacc ttggcctgcg
     8881 ggttcttgtc gtagaagtct ttcagctccg gtagatcgac ggccgacttg cggaccggca
     8941 tgtagcccgt ttgcagcgac caccacgccg tgttctccgg cgtcgaggtg aaggcgacga
     9001 acttgaacgc agcgtcctgc ttttctgagg cggccatcac gcccagaccc gagccgccgg
     9061 tcgggcagcc gaacccggcg ggtccctctg gcaggaaggc agtgccccag tcgaatgtcg
     9121 cctccttgat gaagcgggtg agtccaccag tcgagccgat catcgtcgcc gtcaagccgt
     9181 taaggaagtc ggcatcgacg tcgctcggcg tggaggccca accatccgta accgacgcca
     9241 ggtattggtt gccggcatcg accgcgccgc cctgattgat cagcatgttg aagtcggcgt
     9301 cggagtactg accgccgtat gcccaaacca ggccctgaaa cacccaggcg atgtaattgg
     9361 cggcagtgcg gtgcgcatat gcggcttgtg tcagcgtatc gccgtccttc ttgaccaact
     9421 tcggggcagc ggcagcgagc tcatcccacg tcttcggggc ctcgctcagt ccggcctcct
     9481 ggagagcggc cttgttgtag tagaagagcg gcgtcgagcg ggccatcggc atccagtact
     9541 ggacaccatc gcggttacct tcattgatca gggaatcgac gtagtccgca gggtcgacat
     9601 tgttcgccga catgaggtcg ttgagcggcg ccagggcctg gttcaggtaa aacttgaacc
     9661 accagacgtc cgacaagagg cagacgtcgg gcgtctgctt cgcggcgagg gcggcagtca
     9721 gcttctgtcc cgtctcctcg tagctgccct ggaactcgta cttgacgacg acgtccgact
     9781 gcgccttgtt gaagcgatcg accatgccgg cttcggcgtc gccgttgacc gatcctgggg
     9841 agaatgagcc ccagtaggtg atctcggttg cgctctgggc gatcacattc ggcgcatgga
     9901 ataccgggga gaccgccgca gccgcggccg ttcctgccgc acccttgagg agcgttcgtc
     9961 gattaacccg tgttgtcatg gtgttccctc ccattcagga cccaacccac gtattccgca
    10021 cagaccccat ggcccggcgg taactcgcga gcaggaaagc tcgcggagtt tccaggaaat
    10081 aaactggcga gccgacacgt cgctcacccc ttggtcgctc cagccgcgat tccttcgatg
    10141 atgtatcgct gggcgaagat gtagaccacg atcagcggcg cgatcacgaa gaacaccgcc
    10201 gccatcacca tgccccactg ggtattgccg tcggggtcgt tgagatatga cacaccgacc
    10261 gcgagtggcc gcatccgctc ctcgttggtc acgatcagcg gccagaggta gtcgttccac
    10321 ttggcgacca tcgagatcag gccgaaggtg acgatgatcg gcttggccat cggaatcgca
    10381 atgtcccaga gggtgcgcag gtgcccggcg ccatcgactt tggcggcgtc gagcacctcg
    10441 cgaggcaacc cgagaaaacc ttgccggagc atgaaggtac cgaaggcgac ggccgcaccg
    10501 ggcaggatga tgccctggta ggtgttgatc cagttggagt ggatcagggg aacatcggag
    10561 atggtcaggt agttcggaat gatggtcaat tgcgtcggca ccatcagcgt tgccaggatg
    10621 atgacgaaga ccaggttctt gaacgggaat ctcaggaagg caagggcgta tgccgtcagc
    10681 gtggcattga cgagctcaag accggttccg aagagcgtcg tgacgatgct gacgatgtag
    10741 aaccggtcaa acggtgcggc gttccaggcg tcctgaaagt ttgcccagcg cgggttggat
    10801 ggaatccact gcggtgggaa ggtgtaaatc tcggcgttcg acttgaccga tgccgtcagc
    10861 atccagtaga ccggcgctcc gatcgcgatc accagcacga cgagtgccag gtacccgagg
    10921 agtcgccacg cccggaccct ggtccgttgc gaaagccagg tgcgctcctg actgccgata
    10981 ctgatcgcct caaccgccat agtgaacccg ccgttcctga taccgcaatt gcacgagcgt
    11041 gatcgccaac atgatcacga agagcacgac agcggccgcc gatgccctgc cggcattgaa
    11101 tgccacgaag ccctcgtcat agacgtagta gatgagcgta ttggtcgcgt cgatcgggcc
    11161 gccctgcgtc atgacgcgga tgatgtcgaa ggcctggaac gagttgagga tcgaagtgat
    11221 caccaggaag aaggtgatcg gagagagcat cggcatcgtg accgaccgga agcgccagaa
    11281 cggccccgcg ccgtcgacgc gggccgcttc gtagagatct ttcggaatgc cctggaggcc
    11341 ggccaggtag atgatggccg cgaatcccag attcttccag atgtagacga tgatgattgc
    11401 cggcagtgcc caggaggtgt cgcgcagcca gttcggcgat ccgatgccga gcaggctcag
    11461 gaccgtccgc atcaggccat agcgtggatc gaagatatag acccagacga tgccgagcgc
    11521 cgcgccgctg agcagggtcg gcatgaagac gattgcccgc gcagtgttac gtccgcgcag
    11581 cttctggttc aacaggagcg ccgcgaggag cccgagcagc attgacccgc cgaccgcgcc
    11641 gactgtgaag aacagcgagt tccacatcac cttgtggaac gtgtcgttgt gcaggagata
    11701 ctcgtagttg cccaatccaa ccgggcgctt gcggggcgca agcatgtccc agcggaccgt
    11761 gctcaggtag aagctgtaga tcaggggcca ataggtgaag atcccgaaca ggatcaggtt
    11821 cggacctacg aagagcagga acaggagcca ctcggatgag gctttgcgct tcactgcgcg
    11881 aatcgaatct gccaccgttg gcaaccagac tccacggtaa tcgacaaacc ggctcttctc
    11941 atcaagcatg aggtctgcgc ccaaatgtaa catccctgca cgatgtaaag cagcgcatac
    12001 acgctacaga ggagcgaata atcggcgagg tgtcctgccc gaacggtagg cggcggtggt
    12061 taatgagcgg ttagcggccg gtaaacgaat cggtcgcgcc ggttaattcc cgtgattcgg
    12121 agcagaaatt gatgggaatc cccgatctag atcacgcgcg cttcgagatg catggcttcg
    12181 atatcgagct cgcgctgaag gctgtgcaga actgcatcac tgatcacatc ctggtcgcgg
    12241 agctggacca acgtctggtg ctcgacatcc agaagctcgc gtcgcaggtc ccgcacgctc
    12301 tgcacccacg actcgtcgcc cggcgcggac gatgcgctag tccgcgtcag caggttctgg
    12361 tagtggcgtc gaagcgtgcc gtacacctcg ttgcccgcga aatcgcgcga gagccgatcg
    12421 agctgtttca gagccgccct ggtcatcgcc ccgcgggcgc gctgctcctc gcgcagcgag
    12481 cgatcgccgc gggtcacgcc gagtgccgtg atcagcgcag gcagggtcag cccttgaccg
    12541 accagcgtga cgatgatcac cacaaaggtc aggaactgaa tcagcggacg ctccgtgaac
    12601 ataccggtat tcgtcaccca gggaagtgag agtgccgtcg ccagcgccac gacgccccgc
    12661 atcccgcacc agccgacgat cgtcatctcg cgcacgtcga gctcctcttc ccgaccggtc
    12721 aaccgttggg gaaggagccg gatcgcccct gcgccgaggt agacccagac catccgcacc
    12781 ccgatcaccg ccagcgagat cagcagacca tccttgatca ggctggaggt cgagcgctca
    12841 tcgatcgcgt cgaggatgct gctgaattgg agtccgatca ggatgaagac gagcccgttg
    12901 atgatgaatg tcgccatctg ccagatcacg gctgtcccca gccgctcctc ggaggtgagg
    12961 tcagtcggcg ctttgcggcc ggaatagagc ccggttacga cgacggccag gatcccggag
    13021 acatggatcg attcggccag gatgtagacc acgaagggcg tcatcaaccg cagcacgccc
    13081 gaaaccctgg cctcatcgat ctggtgctgg agcaggtaga gcacccaccc ggaggcaagt
    13141 cccacgagcg ctccgccgac ggcgacgaaa acgaactcca gcgacgcgtg accgagggaa
    13201 aacgagccgg tcttgagcgc ctcaatcgct gcagcgaaca cgaccaatgc agttgcgtcg
    13261 ttgaccaggc tttcatcctg gacgatggcg atgaccttcg gcggcacgcc catccgcttc
    13321 atcaccgcgc tggccgccac cggatcggtc gacgaaacca ccgcgccgag ggtgaaggcg
    13381 gccgtccacg acagatgaag caccgagtgc gcgaggatgc cgacgcagag cgtcgtggcc
    13441 acgaccaggc cgattgccag cagtgtgatc gtccttatat cggcgcggaa gtcgcgccac
    13501 gaggtcgacg cggcatccca atacaggagc ggcggcagga agaggacgaa gacgacgtgc
    13561 ggatccagca cgatgaccgg cagtccgggc acgaagctga ggaccagacc gccgaggaca
    13621 agcaggatcg gataggggat cgagagccgg cccgcgatcg acaccaggac ggcaacggcc
    13681 gccagcaaga tcaggaccag ctcaacttcg cgcatgcgcc gccttcaggt cggaacggtc
    13741 ccggaacctc cggaaatccg gagcaatcgt cccaaattgc cgcggaacga cgcccttgcg
    13801 cgctagggcg gtgagtcgtc tccaggaatc ggggtcagga atgacgcggc tattgggaga
    13861 tcggagcgcg cgcgatgccg ccaggagtag atccacatga aaatcgccca gatcgccgtg
    13921 gcccagacga tcccggccag gacatcgacc gcccaatgct gcgagagata gaccaccgag
    13981 aaccacaagg cgacgttgta cggaaccagc agcaggcccc accagcgaaa tgtcgtgcag
    14041 gcaatcagca gcaccagcca cggaaaggcc gcgtgcagcg acggtagcgc ggcgaccgga
    14101 ttcgggctcg ctcgcgtcca gagccggaac gtgtcgtagt tctcccagcg atgcggaaga
    14161 aaggccttgt acgcctggta ggacggccga tcgacgccgt cgatgtaccc ccaatcggcg
    14221 gccagccagg gcggcgcggc cggcaccagc aggaagaaga cgaacgcccc gtagctcatg
    14281 gccaggagca tcacgacgaa gcggcgaaac atcgtccggt ccttcagcca gagcgcgaag
    14341 ccgaatgcca gcggcaggac gaagtggagc atgtaaatca gggccgcgcc ggtgtcgtac
    14401 cagtgggtca cacccggcgt atagagccgg tcctgcaacc agagcgtcgg gatgtgcccg
    14461 aagaagagcg ccttgtcgcc atcgatcagc gattggacca ggatccggcc gtggtcgttg
    14521 gcgttatacg cgccgaggtt ggtcatgttg tcggcgacac cacgcatcag ctcgtacccg
    14581 aagaggagga gcaggaacgg gccccagtcg cggacaaagg ctttgccctg gccgaggacg
    14641 atggcgccgg ccagcagcag cacggcgatc cggtccggcg tcaggaaaga cccgccgcgg
    14701 atgtaggaga gcgccgtccc ggccacgacg tagataacga agagcgccac gatcacgcgg
    14761 ctttgcgtca ccgcctcgcg tcgttctcgc tcattcgcga ttgtcggact ggaggcgtcg
    14821 atcatgactc ggcgtccacc ggtgtgccgt tctccgggat gtcggtcgtg atctcgaacg
    14881 acccgatggt tacgggcgac ctggcgtttt ggtcgattgc cgcgcgcact gcctgcaccg
    14941 tctcatgcgt ctcgccatcc cgcatcctga tctggatctg gtaggtgccc ggcggcagat
    15001 cgtcgggaag atcgatattg acgatcgatc gcatctgcga aggcacaggc gactctgcac
    15061 gaagaccctt gcggatctcg ccgacgacgt tccccgccga atcgacaacg cgggcgtcga
    15121 tccggtaata gaagggctgg ccgcccgtct cgaagaacag gcggaggcgc aggtcggagc
    15181 cgacctcgat ctgctcgcgg tagacgctcc cgaccaaagt agcctgcccg cgccaggtga
    15241 agatcggagc gtcgggttga tagtagggtt ccggcacgcg tagtccgctc agatcgtaga
    15301 ttcgggcata gacccggcca tcatcctgga taaccgcaac cggcgattga ctgtcgaacc
    15361 acacaatcat gctcagagca taaaactgcc gctggatctc gggctcggtc acgacgacgt
    15421 actggctttg catgaaccat gccgcgcccg ccgggttcag gtcatggttg gccgaaagga
    15481 gcgggaacgg gaggtagtaa tcgatggcgc gtggccaggc gccgccggcg atggtgccgg
    15541 gcgtgatctc gggatgctgc aggatcgtct ccgccaccgc cttgccacct tctccccatt
    15601 ccagcgagat cgagcgccgc gccgcgcttt gcccgcccat caacggtgag acatacgtga
    15661 tgtagtacgg cgcggactca cgcgcggcga ctgcttgccc tcccagcagg accaggaacg
    15721 cggcaaccgc gataccggtc gcgacgtacc gactgattcc cgcccgctgg ctgaccagat
    15781 accggacgag ggcaatcgtg ccgattacgg cgatcagatc gagggccgcc agcgttggca
    15841 acaggtaacg atcgagcttc ttggcggcga acgacaggat cacgaaatag gctgccgcga
    15901 agaccgccag gttgaagcag agacgccggc ccggctctgc ggccccaaca cgcggtgcga
    15961 gcagcccgaa tgttgaaagc accaacgcca cggcggtcag cgggctcaac cggtacgcca
    16021 gcacgacgag gtagtacgac caccccgggt cactcccggt gacctgcccg cggaacagca
    16081 gttggcgggt gtgcggcgct gaggcaaggg tcgagccgcc gcgcacaagc tgatcgatcg
    16141 tatcgatcgg cgcgacccac aacgccggcc aaagcgcgaa gacggtcgca agcgcgatca
    16201 gtccccagac aatcatccgg acgaaatgaa agcggacctg gccgcggatc gggatctgct
    16261 ccgatgctgc gcggcggtag atgtcgaaga gcgtgagcac gaccatcaac ggcagcaaga
    16321 caccattcac cgaccgcgtt aacagcgcca gtccggccgc gatgccggag acgaccagga
    16381 atccccatga gcgacgctgc agaaacgacg tgtacgagac cag
//
LOCUS       DATAOZ010000448         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4654093, whole genome shotgun sequence.
ACCESSION   DATAOZ010000448 DATAOZ010000000
VERSION     DATAOZ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4654093"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VHR64_11505"
     CDS             complement(<1..332)
                     /locus_tag="VHR64_11505"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX3303500.1"
                     /translation="MKSVIYSRTGDPSVLRLVDRDITEPGPGEVRVRIAVSGVNPTDW
                     KSRLGSGSGAAPPFPEVTPNQDGSGVVDALGEGVDHLAVGDRVWVYLAGHQRPTGTAQ
                     QYTNLPASR"
     gene            complement(340..>1747)
                     /locus_tag="VHR64_11510"
     CDS             complement(340..>1747)
                     /locus_tag="VHR64_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX3303501.1"
                     /translation="VHNLLTHGFAADLTDAAARALRVGLDLEMAITDPAYAHLPEAVE
                     AGLVEEDALDASVRRILAAKIRLGLLDDPYVDEDRAREVLADPAHRAVARTAAQRSAV
                     LLRNEGDLLPLTSPGSIAVIGPLADSPRDTIGPWVFDYDLAETVTVLQGIRERAGDDV
                     KISYAPGIRPAQRTFPSMFDMWGNNAPVDPADFDDAAELQRCVATARDADIAVVVLGE
                     WQNMIGEAASRSSLELPGEQLPLLQAVSATGTPVVLLIMNGRPLDLRWAADNVPAILD
                     IWYPGTQGGAAVADLIFGDVAPGGKLPFTWPRTVGQVPMIYSHTISHEPDNQGRRYWD
                     EQSTPLFPFGHGLSYGRFDYTDLALDRTSITAGETVTVSVTVTNSGPRAGDEVAQLYI
                     SQRYGSASRPVRELKGFQRLSLAAGESQTLRFSLGPDELRYWNAASKDWFIDVTAIDV
                     FVGSDSTADQTDSFMITG"
BASE COUNT          328 a          557 c          583 g          279 t
ORIGIN      
        1 cggcttgccg gcagattcgt gtactgctgc gcggttcctg tcgggcgctg gtggcccgcc
       61 agatacaccc acacccgatc gcccaccgca aggtggtcga ccccctcgcc cagtgcatcg
      121 acgacaccgg aaccgtcctg gttaggagtg acctccggga agggcggcgc tgccccactg
      181 ccagagccaa gccgagactt ccaatcggtg ggattgacac cggagacagc aatccggacg
      241 cggacttcgc ccggccctgg ctcggtaata tcgcgatcga ccagccggag aaccgacggg
      301 tcaccggtac gcgagtagat aacggacttc atgaatgcgt caccccgtga tcatgaaact
      361 gtcggtctga tccgcagtcg agtcgctacc cacgaagaca tcgatggcgg tgacatcgat
      421 aaaccagtcc ttcgatgcgg cgttccagta gcgcagctcg tcgggaccca gcgagaagcg
      481 cagcgtttgg gactcgccag cggcgagact aaggcgctgg aagcccttga gctcacgtac
      541 gggccgcgat gccgatccgt aacgctggga aatgtagagc tgcgcgactt cgtcccctgc
      601 gcgaggacct gagttggtca cggtgaccga gacagtaact gtctcgccgg ccgtaatgct
      661 ggtgcggtca agggccaggt cggtgtagtc gaaccggcca taggacagac catgtccgaa
      721 cgggaacagc ggggtgctct gctcgtccca gtagcgccgc ccctgattgt cgggctcatg
      781 cgagatcgtg tggctataga tcatcggtac ctggccgacg gtacgcggcc aggtaaatgg
      841 cagctttccg cccggagcga catcaccgaa gatcagatcg gcgacggctg cacccccttg
      901 agtgccggga taccaaatgt cgaggatcgc cgggacattg tcggcggccc accgcaagtc
      961 gagcgggcga ccgttcatga tcagcagcac gacgggcgta ccggtggccg atacggcctg
     1021 caacagagga agttgctcgc ccggcagctc cagactcgac cgcgacgccg cctcaccgat
     1081 catgttctgc cactcgccaa ggacgacgac ggcgatgtct gcgtcgcggg ccgtggcgac
     1141 gcagcgttgg agttcggcag cgtcgtcgaa gtcggccggg tcgaccggcg cattgttgcc
     1201 ccacatgtcg aacatggagg ggaaggtgcg ctgggcggga cggatgcctg gggcgtaact
     1261 gatcttgacg tcgtccccag cacgctcgcg aatgccctgc agcacagtca ccgtctcggc
     1321 gaggtcgtag tcgaagaccc acggtccgat ggtgtcgcgc ggcgaatcgg ccagcggacc
     1381 gatcaccgcg atggagccgg gactggtgag gggcagcaga tcaccttcgt tgcgtagcag
     1441 caccgcggat cgctgggcag ccgtacgagc cacggcgcga tgggccggat cggcaagcac
     1501 ctctcgggcc cgatcctcgt caacgtacgg atcgtcaagg aggcccagcc ggatcttggc
     1561 cgcgaggatc cgacggaccg acgcgtccag cgcatcctcc tcaacgaggc cagcctcgac
     1621 tgcctccggc agatgcgcgt acgccgggtc ggtaatcgcc atctcaagat cgagcccgac
     1681 ccgcagtgcc cgcgcggcgg cgtcggtgag gtcggcggcg aagccgtggg tcagcagatt
     1741 gtgtacg
//
LOCUS       DATAOZ010000449         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7286162, whole genome shotgun sequence.
ACCESSION   DATAOZ010000449 DATAOZ010000000
VERSION     DATAOZ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7286162"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..548)
                     /locus_tag="VHR64_11515"
     CDS             complement(87..548)
                     /locus_tag="VHR64_11515"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX3303502.1"
                     /translation="MTPEEKLKELGYDLQEPNPYPPAMVAGKIVGNLLFLSGATPPSR
                     FGTTWKGRVGDTYTVEQGYQAAREVAVVQLEMAKHVLGGDLGRIKQVVKVLGMVNCAP
                     GFTDTPKVMHGFSELLHEVLGERGRHARSAVGMQALPGDVPIEVETIFEIE"
     gene            632..>2003
                     /locus_tag="VHR64_11520"
     CDS             632..>2003
                     /locus_tag="VHR64_11520"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303503.1"
                     /translation="MTPRITRRALLATPAIVLAPVSAGTAAPVVAVDLAAPELVPFQR
                     SDVGTVGIFDIDWLSTPKFSDLLDALASSPGAFAGVRCFGTFTAGRPDLYTPETRGAV
                     WIDPAEPIDFSATFAALEQLTTRGLIPFIALGFFPPAISPSPIQPPATWDGWQELVRA
                     FLIALATDPRFGAEAIADWWFEVWNEPNEGRFWQGTTNQYFELYRATSEAVDETGLRI
                     RLGGPAIAYKPQASPADGAPWIEQFLRYVAAHDNIRIDFISLHRKGTVGADPPDPRRL
                     HDAAIETARLAAAIIPERLPSLTIINDEADEKVGFEVPYAPRMDHRNAAWLSASTVLH
                     AQLDQQYAATDLRFMAAADNANLQLVRAPFDGRRSIMTIASTASDELIKLPAFAAYEL
                     LRLLGDRIAPVTSGSDQLYPAADLYHLATISETHAASLLTCYPDPDRPGQPERGIGYQ
                     LDGLPWE"
BASE COUNT          324 a          706 c          612 g          361 t
ORIGIN      
        1 tgccacggca aacccctaca ggagtggttg gtcgttggtt ttcggtttcc gtatctaacg
       61 accaaccacc ctgaaccttc catccgctat tcgatctcga aaatcgtctc cacttcgatc
      121 gggacgtcgc cggggagagc ttgcatgccg acggccgagc gggcgtgacg gccgcgctcg
      181 ccgaggacct cgtgcagcag ctccgagaag ccgtgcatca cctttggcgt gtcggtgaag
      241 ccgggcgcgc aattgaccat gcccagtacc ttgaccacct gcttgatccg gcccagatcg
      301 ccgccaagga cgtgctttgc catctcgagc tggacaaccg ccacttcgcg cgccgcctgg
      361 tagccctgct cgacggtgta ggtgtcgccg acgcggccct tccaggtcgt gccgaagcgg
      421 ctcggcgggg tcgcaccgga gaggaagagg aggttgccga cgatcttgcc ggccaccatc
      481 gccggcggat atgggtttgg ctcctgcagg tcgtagccga gttccttaag cttctcttcg
      541 ggggtcatcc aatgctcctt cgcactatca cgcacaaatc acgtcccggc attatcccgg
      601 atttcgccgc gcccgcccgc ccggcatgcc gatgaccccg cgcatcacga gacgggcact
      661 cctggccacg ccggcgatcg tgctggctcc ggtgagtgct gggacagccg cgccggtcgt
      721 cgcggtcgat ctggccgcgc cggaactggt cccgttccaa cggtcggatg ttgggacggt
      781 cggcatcttc gacatcgatt ggctcagcac gccgaaattc tcggacctcc tcgatgcgct
      841 cgcctcctcg ccaggcgcat tcgccggggt gcgatgcttc ggcacgttca ctgccggtcg
      901 gccggacctg tacacgccgg agaccagagg cgcggtctgg atcgatcccg ccgagccaat
      961 cgacttctct gccaccttcg ccgcgcttga gcaactgacc acgcgcggcc tgatcccgtt
     1021 catcgcgctc ggcttcttcc cgccggccat ctcgccatct ccgatccagc cgccggccac
     1081 gtgggacggc tggcaggaac tggtccgcgc cttcctgatc gcactggcga ccgatccccg
     1141 cttcggcgcc gaggcgatcg cggactggtg gttcgaggtc tggaatgagc cgaacgaggg
     1201 acgattctgg caggggacca cgaatcagta cttcgagctc taccgcgcaa cctcggaggc
     1261 ggtcgacgag actgggctgc gcatccggct cggtggaccg gctattgcct acaagccgca
     1321 ggccagtccg gccgatggcg cgccatggat cgagcaattc ctgcggtacg ttgccgcgca
     1381 cgacaacata cggatcgatt tcatctccct gcaccgcaag ggaaccgttg gcgctgaccc
     1441 gcccgatccg cgccgcctgc acgacgccgc gatcgaaaca gcccggctcg ccgcggcgat
     1501 catccccgaa cgattgccca gcctgacgat catcaacgac gaggcggatg agaaggtcgg
     1561 cttcgaggtc ccgtatgccc cgcgtatgga ccaccggaac gcggcctggc tgagtgcatc
     1621 gaccgtcctg cacgcgcagc tcgatcagca atatgcggcg accgatctgc gatttatggc
     1681 cgctgccgac aatgccaacc tccagctcgt gcgggccccg ttcgacggtc gccgctcgat
     1741 catgaccatc gcctcgaccg cgagcgacga gctgatcaag ctgcccgcct tcgccgccta
     1801 tgagctgctg cgtctcctcg gcgatcgcat cgctccggtg acgtcgggct cagatcagct
     1861 ctacccggcc gccgacctct accacctggc aacgatttcg gagacgcacg ctgcgtcgct
     1921 gctgacctgc taccccgacc cggaccgtcc cggtcagcct gagcgcggga ttgggtacca
     1981 gctcgatggg ttgccatggg aga
//
LOCUS       DATAOZ010000450         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5706830, whole genome shotgun sequence.
ACCESSION   DATAOZ010000450 DATAOZ010000000
VERSION     DATAOZ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5706830"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2366
                     /locus_tag="VHR64_11525"
     CDS             <1..2366
                     /locus_tag="VHR64_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX3303504.1"
                     /translation="VGATDGGLALIRSGNEADDFRVVRVFTDADGLGSNWINQVLQTS
                     DGEVWIATYAGVVRMRRDAATGRCAPCADPIMVGATSPTISLAEDRSHNVWVGTSTGV
                     ARVLRVGFALFSAADGIPAASSLIETTDGVVLAMTAGTTREGAAWFDGQRFVPIQLPV
                     GAAVASWGWNQVMVESRYGDWWIGTRAGAIRLKGVDATTHLHRARERHRLSRTDGLAA
                     DVVLRLFEDSRGDVWIGTAGEGKGNGLSRWERSTGTLHHYESIPGLPDLGRYFVTAFA
                     EDRSGAIWIGFNDEGGLARLRGGSIDRFAEPELGEIGSVRNLLVSSDGSLWGATTRGG
                     LLRAVTSFADRPYIRRLTVADGLSSNDVGAVVEDSGGAIYAGTGRGIDPIDRTSERVF
                     RYGPADGLPVGQVNAAIRDRRGALWFAYNLGIVKLIPLASQARAVPSVVIDGLTINDG
                     PRHLSALGQVDVNSFELPPGRTSLQVSYLAPGLGPMDHVRYQIRLEGVDRDWSQPSDQ
                     RTVSYANIGPGQYRFAVRALTSDGLVTPNVAGFEFRVLAPIWERWWFLPTALLFAGAT
                     GYGVNRYRTARIVQLANIRSRIARDLHDDIGANFTRIAVLAEVVRRQKTLMPAADAPL
                     SSIANVARESMTAMGEIVWAVNPDRDRIGDLVGRMREYAEEVFVAEEVSVTFTVPEGL
                     KDVRLRGDARRDVYLVFKEAANNAARHSGCSSFHVEMARDRRWLAISISDDGRGFDGA
                     DGDGNGLSNMRRRATIAGGTLTLDAAPGRGTTVTLTVPLGVRARLFG"
BASE COUNT          455 a          831 c          886 g          543 t
ORIGIN      
        1 gggtcggcgc aaccgacggc ggcctcgcgc tgattcgctc cggcaatgaa gcggatgatt
       61 tccgcgtcgt gcgagtcttc accgacgccg acggtctcgg cagcaactgg atcaaccagg
      121 tgctgcagac ctccgacggc gaggtctgga ttgccaccta cgcaggagtc gttcgcatgc
      181 ggcgtgatgc ggcgaccggg cggtgcgcgc cctgcgcgga tccgatcatg gtgggggcga
      241 cttctccgac gatctcgctg gccgaggatc gcagccacaa tgtgtgggtc gggacgtcca
      301 ccggcgtcgc gcgggtcctc agggttggct ttgcgctgtt ttccgccgcc gacggcattc
      361 ctgccgcatc ctcactcatt gaaaccacgg acggtgtggt tctagccatg accgccggta
      421 cgacgcgcga gggcgccgcg tggttcgacg gccagcgatt cgtgccgatt cagctgccag
      481 ttggtgcggc agtcgcgagc tggggctgga accaggtcat ggtcgagagc cggtatggtg
      541 actggtggat cggtacacgc gccggcgcca tccgcctgaa gggtgtggac gccacgacgc
      601 acctgcatcg tgcccgggag cgccatcgac tctcgaggac ggacgggctg gccgccgacg
      661 tcgttctgcg gttgttcgag gattcacgcg gcgatgtgtg gattggcacc gccggggaag
      721 ggaagggcaa cggactctcg cgctgggagc gatcgaccgg gaccttacac cactatgaat
      781 cgattccagg tctgcccgac ctcggtcgat atttcgtgac cgcgttcgcc gaggatcgtt
      841 ccggcgccat ctggatagga ttcaacgatg aaggcgggct cgctcgcctg cgtggagggt
      901 cgatcgatcg ttttgccgag cccgagctgg gagaaatcgg gtccgtgcgc aacctgctcg
      961 tcagctcgga cgggtccctt tggggcgcga cgacgcgagg cggcctcctg cgtgccgtca
     1021 cgtcatttgc cgatcgccct tacatccgcc gtttgaccgt cgccgacggg ctttccagca
     1081 atgatgtcgg cgcagtggtc gaggacagtg gcggcgcgat ctatgccggg accgggcgcg
     1141 gcattgaccc gatcgatcga accagcgaac gagtcttccg gtatggccca gcagacggtc
     1201 tgccggtcgg tcaagtcaac gcggccatac gggaccgccg cggcgcgctg tggttcgcct
     1261 acaacctcgg catcgtcaag ttgatccccc tcgccagcca agcccgcgcg gtcccatcgg
     1321 tcgtgattga cggcctcacc atcaacgatg gcccgcggca tctctcagct ctcgggcagg
     1381 tcgatgtcaa ctcatttgag ctgccaccag ggcggacctc tctgcaagtg agctacctgg
     1441 cgccagggct gggcccgatg gatcacgtcc gataccagat ccggctcgaa ggagtcgacc
     1501 gggactggag tcagccgtcc gatcagcgaa ccgtcagtta cgccaacatt ggtccgggtc
     1561 agtatcgttt cgccgttcgc gccctgacgt ctgacggact cgtgaccccc aacgtcgccg
     1621 gtttcgagtt ccgagtgctg gcgccgatct gggagcgctg gtggttcctg ccgacggcac
     1681 tcctgtttgc cggcgccacc ggctacggcg tcaatcgcta tcggactgcc cggatcgtcc
     1741 agctcgcgaa cattcgctcg cggattgctc gtgatctcca cgacgatatc ggcgcgaact
     1801 ttacccgcat cgccgtgctc gccgaagtcg ttcgccgcca gaagaccctc atgccagcag
     1861 cggacgctcc cttgagctcg atcgcaaacg tcgcccgcga atcgatgacc gccatgggtg
     1921 agatcgtgtg ggcggtcaat ccagaccgcg atcgcatcgg cgacctcgtc ggccgcatgc
     1981 gtgagtatgc ggaggaggtg ttcgtagccg aagaggtgtc cgtgaccttc actgtgcctg
     2041 aaggcctgaa ggatgtccgt ttgcgtggtg atgcgcgacg agatgtttac ctggtgttca
     2101 aggaggcggc taacaacgcc gcacgacatt cgggatgttc cagttttcat gtcgagatgg
     2161 cccgggaccg tcgatggctc gcgatctcca tcagcgatga cggccgggga ttcgatggcg
     2221 ccgacgggga cggtaatggt ctttcaaaca tgcgccgccg ggccacgatc gccggtggca
     2281 cgttgacact ggacgccgcc ccgggccgtg ggacaaccgt cacgctcacg gttcccctgg
     2341 gggttcgcgc ccgcctgttt gggtagtggg tcagtttcgg aatttgactt tacattgtaa
     2401 agtaatttgt tacaattaga gtgccactgt gcaattccgt aacccggacg taaacgtctt
     2461 gacctaaatg tctgcatggt tgtaacttac aaacagctat gtcctttggt ggcctcgtcc
     2521 gacgaatctt cggcggcgtg accagtcctg cccccagcaa ccggctgcac gtcgaatatc
     2581 aacctcaggc tgatctgctc cttgcttggt ttggcgagcc ggagagagcg cgcaacattg
     2641 aggttgagcc tggagtgtac gtgcgcgttg cgaaggacga agaccgcgtg atcggtatcg
     2701 agattcttga ttgcg
//
LOCUS       DATAOZ010000451         4712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8600896, whole genome shotgun sequence.
ACCESSION   DATAOZ010000451 DATAOZ010000000
VERSION     DATAOZ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8600896"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VHR64_11530"
     CDS             <1..781
                     /locus_tag="VHR64_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX3303505.1"
                     /translation="YPDGSVGKAGGLVVKNVSGFDMMRIHYGALGTLGVITSANFKVL
                     PVPRSEFTVNHPIADIADLADLLPRLRLPSVRPVSLVVGREANQWLLAARYEGRRSGL
                     EAVESAIAGFLDLSGKLSDAASSHYWQRLMDARAFTETMEVRLQLRSLPTEIVSITGL
                     AIEIAEHAGLRLSRVEIEPGNGLATMSWPHRDAASALVIGGIREQLRRATVTVQAAPD
                     EVKRSIDVWGDEPEAIDLMRRLKQEFDPNRVLNPGRFAGRI"
     gene            792..2150
                     /locus_tag="VHR64_11535"
     CDS             792..2150
                     /locus_tag="VHR64_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX3303506.1"
                     /translation="MAIAEHADLKRLGGFSGHDIPDPDLLKACVHCGMCLSSCPTYRL
                     TGQEMSSPRGRLWMMSAVAENRLDLLDPAFTEQMYQCLNCRACEAVCPSGVQYGPLVE
                     ASRAQIEQHQTRPVHQRALRRVALGWLFGEAGRFRAFTRATALYQKSGLSFLAHRTGI
                     LRLLKLEDSEAMLPPIKARPLQPGTESWTPRTARERAVLFNGCIMGTVFADVNRAAGR
                     VMAHNGIAVEVPVKQQCCGALQVHSGMMDEARDLARRNIEAFEASGNETIVVTAAGCG
                     AALKEYGHLLHADPVYAERARDFSSRVKDVNEYLGSQPIVEPSRPVKRTVTYQEPCHL
                     AHAQRVTVQPRTLLKSIQGVELIEMKESSLCCGSAGIYNILRKDFADRLGDRKAANTK
                     ATGASTVVTANPGCFLQLRSTLTRNGADMDVKYVVELLDEAYGGSGVTEWAIDRSTHR
                     SS"
     gene            complement(2107..3801)
                     /locus_tag="VHR64_11540"
     CDS             complement(2107..3801)
                     /locus_tag="VHR64_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX3303507.1"
                     /translation="MTSRSLDFASTGSRTVTLLRTLNSIGRLSSTAASEVAIAEFVTT
                     SVVDVVGGDGCAVFINQPGTDRLQIRAALGFATEKINEIHLSIGEGIAGKAASDRETI
                     LAPDARNHPNFLAVPGSGEERFRSEIATPMVTTDTNEMIGVLCLFSVKQRSFDDETVE
                     FLEAIAREAAHAIVAARMRSASEEQLRKKVAELSTLQRVSRMIASSLDLDEVLRSIAT
                     ASVEIIDAEAAAVFRLPPPTVADPEEPSLVMDYRVGEIRPLVDPSSRTRLVMQVIRSG
                     AARITELDYIGGSNLLFCMPLRSARGTMGALCIRLRPGIELAEDELGLLQAFTDTASL
                     AIENAQLYEEARASVQKSSTLLQEMHHRVRNNLQTVAALLSLQLRETQSSETTDALRE
                     AVSRVQSIAAVHDLLSDEGRLAGATVGAIARLVVEQASMTFIHPPLRVTFTIEPSNLV
                     VSSRQATVLALLINELVANAVEHGFRDRPTGHIWIRAETKGHMCTLDVENDGEPLPPT
                     FDVAEAKGLGMRIVERLVTSDLGGQFVMAGTDTGTRVRLSFPITTNDALSDLSPTR"
     gene            4023..>4712
                     /locus_tag="VHR64_11545"
     CDS             4023..>4712
                     /locus_tag="VHR64_11545"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX3303508.1"
                     /translation="MTIGSASSDAYLHRGTSQDNSIIYRQPTGRALATNADLRGQSDT
                     ALQSSINFLSGAGYRFVRQEFSWSQFEPAPGQFDWTEYRRIVDALSAAQIDVIVVLVD
                     TPLWARAPASVSFADAPPLSDQIYEQMCTALRAEFPELHLFQIGQNLDDPEYWGGKSL
                     QALPYRTLLTAAARGLDIAATDSTLISGEVSMNPEIRKSGGDIEVLERLFRDPGIRGL
                     IRVVGVSADGGT"
BASE COUNT          858 a         1476 c         1520 g          858 t
ORIGIN      
        1 gtatccggac ggctcggttg gcaaggccgg cggccttgtc gtcaagaacg tttccggttt
       61 cgacatgatg cgaatccatt acggggcgct ggggacgctg ggagtgatca catcggcgaa
      121 cttcaaggtc ctgccggttc ctcgatcaga attcaccgtg aatcatccca tcgcggatat
      181 cgccgacctg gccgatctct tgccgcggct gcgactgccg agcgtgcgac ccgtttcgct
      241 ggtcgttggc agggaagcga atcaatggct gctcgcagca cgatacgagg ggcggcgatc
      301 gggactcgag gccgtcgaat cggcgatcgc cggcttcctt gatctctcgg gcaagctcag
      361 cgacgccgca tcgtcccact actggcagcg cctcatggat gctcgcgcat tcaccgagac
      421 gatggaagtc cgcttacagc tccggtcgct gccgaccgaa atcgtttcca taaccgggct
      481 cgccatcgag atagcggagc atgccggcct gagattgtcc cgcgtggaaa ttgagccggg
      541 caatgggctg gcgacgatgt catggccgca ccgcgatgcg gcaagtgcgc tggttattgg
      601 cgggatcagg gagcaattgc gccgggccac ggtgacggtg caggccgccc ccgacgaagt
      661 caagcggtcg atcgatgtct ggggcgacga gcccgaggcg atcgatctga tgcgtcgtct
      721 caagcaagag ttcgatccga accgggtcct caacccgggc cggtttgcag gaaggattta
      781 ggggcacccg cgtggcaatc gccgaacatg ccgatctgaa acgcctcggt ggattctcgg
      841 gacacgacat cccggatccg gacttgctga aggcctgtgt ccactgcggg atgtgcctct
      901 cgtcatgccc gacctaccgc ctgaccggcc aggagatgtc atctccgcgc ggtcggctct
      961 ggatgatgag cgcggtcgcc gagaatcgcc tggacttgct cgatcctgcc ttcaccgagc
     1021 agatgtatca atgtctgaac tgccgtgcct gcgaggcggt ctgcccttcc ggcgtgcaat
     1081 acgggccact ggtcgaggcg tctcgcgccc agatcgagca gcatcagacc cgaccggttc
     1141 accagcgggc gctgcgcagg gtcgcgctcg gatggctctt cggcgaggct ggtcgcttcc
     1201 gcgcattcac ccgggcgacc gcgctctatc agaagtccgg cctgtcgttc ctggcccacc
     1261 ggacggggat cctccggttg ctgaagctgg aggactccga ggcgatgctg ccgccgatca
     1321 aggcccgacc cctacagccg gggactgagt cctggacgcc gaggacggca cgcgagcggg
     1381 cggtcctctt caacggttgc atcatgggga ccgtttttgc agacgtgaat cgcgcggccg
     1441 ggcgggtcat ggcccacaac gggatcgcgg tcgaggtccc ggtcaagcag cagtgttgcg
     1501 gagcgcttca ggtccacagt ggaatgatgg acgaggcgcg ggatctggct cgacgcaaca
     1561 tcgaagcgtt cgaggcgtca ggtaacgaga cgatcgttgt gacggcggcc ggttgtgggg
     1621 cagcgctcaa ggaatatggg catttgctgc acgccgaccc agtctacgcc gagcgggcgc
     1681 gcgacttcag cagccgggtc aaggacgtca acgagtacct cgggtcccag ccgatcgtcg
     1741 agccgtctcg ccccgtcaag cggacggtga cctaccagga gccgtgccat ctcgcccatg
     1801 cccagcgcgt caccgttcag ccgcgaacgc tcctgaaatc cattcagggg gtcgagctta
     1861 tcgagatgaa ggagtcgtcg ctgtgctgcg gcagcgcggg catctacaac atcctgcgca
     1921 aggacttcgc ggatcggctc ggtgaccgga aggcagcgaa cacgaaggcg accggggcat
     1981 cgaccgtcgt cacggccaat cccggctgct tcctccagct ccgctcgacg ctaacgcgca
     2041 atggcgcaga catggacgtc aagtacgtcg tcgagctgct cgatgaggca tatggcgggt
     2101 ccggtgttac cgagtgggcg atagatcgct caacgcatcg ttcgtcgtga tcgggaacga
     2161 cagccggacg cgggtgccgg tgtcggttcc cgccatcacg aactggccgc ccagatccga
     2221 cgtgaccagc cgctcgacga tccgcattcc aagtcccttg gcctccgcga catcaaatgt
     2281 cgggggcaac ggctcgccgt cattctcgac atcgagcgtg cacatgtggc ccttcgtctc
     2341 ggcgcggatc cagatgtgac ctgtcggccg atcgcgaaat ccatgctcga ccgcgttcgc
     2401 gacaagctcg ttgatcagca aggccagcac ggtcgcctgg cgtgacgaga cgaccaggtt
     2461 cgagggctcg attgtgaagg tcacccggag cgggggatgg atgaaggtca tcgatgcctg
     2521 ctcgaccaca agccgcgcga ttgccccgac cgtcgcaccg gcaaggcgac cttcgtcgct
     2581 caggagatcg tggaccgccg caatagattg gacgcgcgag acggcctcgc gtagcgcgtc
     2641 cgttgtctcg gatgactgcg tctcgcggag ctgcaatgaa agcagcgccg ccaccgtctg
     2701 caaattgttc cggacgcggt gatgcatctc ctgaagcaac gtggagcttt tctggacgct
     2761 cgcgcgtgcc tcttcataaa gctgggcgtt ctcaattgcg agcgaggccg tgtcggtaaa
     2821 ggcttgcagc aacccgagct cgtcttcggc cagctcaatc cccggccgga ggcgaatgca
     2881 gagtgcgccc atcgttccgc gcgcggaccg cagtggcatg caaaagagca ggttggaacc
     2941 gccgatgtag tcgagttcgg tgatacgggc ggctccggag cggatgacct gcatgaccag
     3001 ccgcgtccgg gatgacggat ctacgagtgg ccggatctcg ccaacccggt aatccatgac
     3061 cagagacggc tcttccggat cggccacggt cggcggcggc aaccggaaga ccgcggcggc
     3121 ttcggcgtcg atgatctcga ccgacgccgt ggcgatcgag cgcagtactt catcgaggtc
     3181 gagggacgag gcaatcatgc gggagacccg ttgcaacgtg ctcagctcgg caaccttctt
     3241 gcggagttgc tcctcactgg ccgagcgcat tctcgcggcg acgatcgcgt gggccgcctc
     3301 gcgagcgatc gcctccagaa actcgaccgt ctcgtcatcg aaggaccgct gcttgaccga
     3361 gaagaggcac aggacgccaa tcatctcatt ggtgtcagtc gtaaccatcg gggtggcgat
     3421 ctcggagcgg aagcgctcct ctcccgaccc gggaacggcc aggaaattgg ggtgattgcg
     3481 ggcatccggc gccaggatcg tctcgcggtc gctggccgcc ttgccggcga tgccctcgcc
     3541 aatcgacagg tgaatctcgt tgatcttctc cgtcgcgaac ccaagtgcgg cacgtatctg
     3601 gagccggtcg gtaccgggtt ggttgatgaa gaccgcgcac ccgtcaccgc caacgacatc
     3661 tacaaccgag gtggtcacga attcggcgat cgccacttca gacgcagccg tcgacgagag
     3721 ccgcccgatc gaattgagcg tacgcagcag ggttaccgtc cgcgagccgg tcgatgcgaa
     3781 gtccagcgat cgagacgtca cgagttgtca cctctagcct gagcggaccc ggccacgatc
     3841 gcgctccgag taccattcac cgttgtcaac ccaatgctac gtcactacgg acccttgatt
     3901 tgtcgacaac ggaccggctt ctgacatccc aatcatcgac ttccaggcca cgccggtttg
     3961 cgctttcggc tacgactgcg cgcgcgctcc gagcggcgtt gctgatattg gtcggtctcg
     4021 cagtgacgat tggatcggca agctcggacg catacctgca tcgaggcacg agccaggaca
     4081 actcgatcat ctatcgccaa ccgacgggac gagcactggc aactaatgcc gacctgcgcg
     4141 gccagtccga cacggcactg cagtcgtcga tcaacttcct ttccggcgca ggatatcgat
     4201 tcgtccgcca ggagttttcg tggtcgcagt ttgagcctgc gccgggacaa ttcgactgga
     4261 cggaataccg gcggattgtc gatgcactgt ccgccgctca aatcgacgtg atcgtcgtcc
     4321 tggtggatac gccgctctgg gcgcgagcac ccgcctctgt gtcctttgcc gatgctccac
     4381 cgttgagcga tcagatctac gagcagatgt gtaccgcgtt gcgggccgaa ttcccggagc
     4441 tgcacctctt ccagatcggt cagaaccttg acgatcctga gtactggggt ggcaagtccc
     4501 tacaggcgct gccgtatcgc acgctgctga ccgcggcggc gcggggcctg gacattgcgg
     4561 cgacagacag cacgctgatc tccggcgagg tcagtatgaa tcccgagatc cggaaatccg
     4621 gcggcgacat cgaggtgctt gagcgcctct ttcgcgaccc gggaatccgg ggattgatac
     4681 gcgtggtggg agtctcggcg gatggtggca cc
//
LOCUS       DATAOZ010000452         4181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1232446, whole genome shotgun sequence.
ACCESSION   DATAOZ010000452 DATAOZ010000000
VERSION     DATAOZ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232446"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VHR64_11550"
     CDS             complement(<1..506)
                     /locus_tag="VHR64_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX3303509.1"
                     /translation="MANAWYLHHFYRFQPDLNFANPDVREEFRAIIGLWLQQGVSGFR
                     IDAAPFLIDLTGTGFEPHMETAHAFLRELRDFATVRQGNAVLLGEVNVGLSTIADYFG
                     GGNELQALFSIPLNRFTFLGLAQKNADAIKFGLGQLPTIPDAGQWVNFLRHHDELNLS
                     QLTKEQRG"
     gene            complement(575..1021)
                     /locus_tag="VHR64_11555"
                     /pseudo
     CDS             complement(575..1021)
                     /locus_tag="VHR64_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(1090..1518)
                     /locus_tag="VHR64_11560"
     CDS             complement(1090..1518)
                     /locus_tag="VHR64_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX3303510.1"
                     /translation="MIFLDANLILRYIANVETDDQVRMQRVAGDLMLALEAGIVEVTT
                     SEVVLHEVCFVMTSRRQYGVSVNDAITALEPILRLPALKFPSGDREIYLRALEIWERN
                     PKLEFSDSVIAARCEINGWELATFDETLGSLPNVARWQPM"
     gene            complement(1515..1793)
                     /locus_tag="VHR64_11565"
     CDS             complement(1515..1793)
                     /locus_tag="VHR64_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX3303511.1"
                     /translation="MQKFILRPSSKNQVTIPKPVRQALGVDPKDPIAVVIDDEGKVSL
                     EPARLLSISELKGILPPLGRPTSPDFSAEIREAMEEHAQQIVDRLNQR"
     gene            complement(1892..3139)
                     /locus_tag="VHR64_11570"
     CDS             complement(1892..3139)
                     /locus_tag="VHR64_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3303512.1"
                     /translation="MKHVLIIGSGVIGLSTAYSLKQRGVPEITIIDSGPGPHGASVVN
                     AGWVTPAHSDPVGVPGMTTQALKWMLKSDSPLYIKPAVHDPEFMLWLFKFWRSLNDKS
                     FEHATAANVALNEKTFQLFDEYEASGVKFEMYKEGLIMAFLSGHHLEEGLKNMERYVK
                     YGFPMPKVYWGKDVQGLEPALSSNVNGVFHIEEERHLRPDTLTRGLIEWLGERGVTFA
                     NNNTVVGFDINDGAVRAVEATGGRYEADAFVIAAGAYSGRIAKKAGKGLPVQAGKGYK
                     IDYHNPPTRLTHPLSLHEPRMAVTPMGGFTRLAGTMELSGINTVVRQERVRALSRGGR
                     QFLRDWPEEIPSATVDSGLRPMTPDGMPIIGAMPKLRNLTVSTGHQMLGFGLAPASGD
                     ALAELIVTGKTPAVLEPFTPNRF"
     gene            complement(3317..3688)
                     /locus_tag="VHR64_11575"
     CDS             complement(3317..3688)
                     /locus_tag="VHR64_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303513.1"
                     /translation="MSQSFTGNHFSAGDTVFSSDGQKVGVVAAIHGNYLLVEKGFLHV
                     TDYHVPFSAISRHDAPQGAVFLNVSRDEALHSGWDQHPADLEDAPVDTLLTGAATMPD
                     PPDIEVIEPDDEPLRSEEAAL"
     gene            3967..>4181
                     /locus_tag="VHR64_11580"
     CDS             3967..>4181
                     /locus_tag="VHR64_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303514.1"
                     /translation="MDDQVKDEAAVAETGEQPAAEAGQERYATGVARLDNMVHKIDDG
                     VRRISKEIHSLEVEEDPNRVATGIKPL"
BASE COUNT          792 a         1234 c         1316 g          839 t
ORIGIN      
        1 tcgccgcgtt gctccttcgt gagctgcgac aggttgagct cgtcatgatg ccgcaggaag
       61 ttgacccatt gcccggcgtc ggggatcgtc ggcaactggc cgagaccgaa cttgatcgcg
      121 tcggcattct tctgggccag gccgaggaac gtgaaacgat tcagcggaat gctgaagagc
      181 gcctgcagct cgttgccgcc gccgaaatag tcggcaatcg tgctcaggcc gacattgacc
      241 tccccgagca ggaccgcatt gccctggcga acggtggcga aatctcggag ctcgcgcagg
      301 aacgcgtggg ccgtttccat gtggggttcg aaacccgttc cagtcagatc gatcaggaat
      361 ggggccgcgt cgatccggaa tccgctgaca ccttgctgca gccagagccc gatgatcgcg
      421 cggaactctt cgcgcacatc cgggttggcg aagttgagat cgggctggaa ccggtagaag
      481 tgatggaggt accaggcgtt ggccacctcg tcatatgacc agatcccgtc ctgctctccg
      541 ggaaaacgac gttgtcactg gtgtcgccgg gatcgtcgtc ccgccagatg tagtaggagc
      601 gatagggcga gtccgggtct gagcgtgcct tctggaacca ggggtgctga tcggacgtgt
      661 gattgggcac gaggtcgatg atgatgtgca ggccgcgttc cctcgcctcg accatgaact
      721 cgctgaagtc ggccatggag ccgaaccggg gatcgatcgc ggtgtagtcc gagacgtcgt
      781 agccgtcgtc gcaattcggc gatgggtaaa agggcatgag ccagaggcag gtgacgccga
      841 gacttgcgag gtagtcgagc cggtgcgtca atcccttgaa gtcgccgacg ccatcgccgt
      901 cggagtcggc ataggtctcg acatcgaggc agtagatgat cgcgttcttg taccactgat
      961 cgcgcaggcc cacggcatct ccttgccaac cgattggtcc gacctcggcg gtgcacccca
     1021 ctcactatgc cgatgaatca ggcgggaatg gcgtgccggg acaacaggtt gtttggccga
     1081 cgtttgtcgt tacatgggtt gccagcgggc gacgttgggg agcgagccga gcgtttcgtc
     1141 gaacgtggcc agctcccagc cgttgatttc gcagcgagca gcgatcaccg agtcggagaa
     1201 ttcaagtttc gggttccgct cccagatttc gagtgcgcgc aggtatatct ccctgtcacc
     1261 ggatggaaat ttgagcgctg gcaaacggag gatcggctca agcgccgtga ttgcatcgtt
     1321 gacgctcaca ccatattgcc gccttgacgt catcacgaag cagacttcat ggaggacgac
     1381 ctcggaggtt gttacttcga cgatgccagc ctccaaggca agcatgagat cccccgcaac
     1441 gcgttgcatg cgcacctggt catccgtctc aacgtttgcg atgtatctca ggatgagatt
     1501 cgcgtcgagg aagatcatct ctgattcaga cggtcgacaa tctgctgcgc gtgctcctcc
     1561 attgcctcgc gaatttccgc gctgaaatca ggagacgttg gtcttcccaa gggcgggagg
     1621 atgcctttca attctgaaat tgacaacagg cgagctggct ccagactcac cttgccctcg
     1681 tcgtcaatga caacggcgat tggatctttg ggatcgacac cgagtgcctg tctgacaggc
     1741 ttgggaattg ttacctgatt cttgctactc ggccgaagga tgaacttttg cattacgaac
     1801 cccatgagta aagctaatcc ataatcactt tacccacgag gtagcaagag tgcaactcac
     1861 acgactcagc cggtggtccc gctaactact gctagaaccg gttcggggtg aagggctcca
     1921 ggacggccgg tgtcttgccg gtcacgatca actcagccag cgcgtcgccg ctcgccgggg
     1981 cgagaccgaa acccagcatt tggtgacccg tcgagacggt cagattgcgc agcttcggca
     2041 ttgcgccaat gatcggcatc ccgtccggcg tcatcggccg cagcccgctg tcgacggtcg
     2101 ctgacgggat ctcctccggc cagtcgcgca ggaactggcg accgccgcgc gaaagggcac
     2161 ggacacgctc ctgccgcacg accgtgttga tgccggagag ctccatcgta ccggcgagcc
     2221 gggtgaagcc gcccatcggc gtgactgcca tgcgtggttc gtgcagcgag agcggatgcg
     2281 tcagcctggt cggcgggttg tggtagtcga tcttgtagcc tttgccggcc tgaaccggaa
     2341 gacccttgcc ggccttcttc gcgatgcgcc cggaatatgc gccggcggcg atcacgaacg
     2401 cgtcggcctc gtagcggcca ccggtcgcct caaccgcgcg caccgctccg tcgttgatgt
     2461 cgaaaccgac gaccgtgttg ttgttcgcga aggtgacgcc gcgctcgccg agccattcaa
     2521 tcaggcccct ggtcagcgtg tccgggcgca ggtgacgttc ctcttctata tggaagacac
     2581 cgttgacgtt ggaggagagc gccggctcca ggccctgaac atccttgccc cagtagacct
     2641 tcggcatcgg gaagccgtac ttgacgtacc gctccatgtt cttgaggccc tcttccaggt
     2701 ggtgcccgga gaggaaggcc atgatcagcc cttccttgta catctcgaac ttgacgccgg
     2761 atgcctcgta ctcatcaaag agctggaagg tcttctcgtt cagcgccaca tttgcggcgg
     2821 ttgcgtgctc gaagctcttg tcgttgagcg accgccagaa cttgaacagc caaagcatga
     2881 actcggggtc atgcaccgcc ggcttgatat agagtgggct gtccgatttc agcatccact
     2941 tgagggcctg tgtggtcatt cccggcactc caaccgggtc tgagtgggcc ggcgtgaccc
     3001 atccggcatt gacgaccgac gctccgtgcg gcccggggcc gctgtcgatg atggtgatct
     3061 cgggtactcc ccgttgcttc agggagtacg ctgtgctgag tccgatgacg ccactgccga
     3121 tgatgagtac gtgtttcatg gacggaatga taccgccact tttcacaatg tgagtaagcg
     3181 gattttcgca ctccgacagc gatttcggcc gtcggatagc caacatcggc gattcccgat
     3241 ttgcgcgcat gtcggggtga tgggtcgcgc cgaggcgggg ccggattttg gattcttccg
     3301 tgaaatgtgc gtgtcgttag agagccgctt cctcgctccg gagcggctcg tcgtccggct
     3361 cgatcacctc gatgtccggc ggatccggca tggtcgccgc cccggtcaaa agcgtgtcga
     3421 ccggtgcgtc ttcgaggtcg gcaggatgct gatcccagcc gctgtgcagc gcctcgtccc
     3481 gtgacacgtt cagaaaaacg gcgccctgcg gggcatcgtg gcggctgatc gcgctgaagg
     3541 ggacgtggta gtcggtcacg tgcaggaacc ccttctcgac cagcaggtaa ttcccgtgaa
     3601 tcgcggcgac gacgccaacc ttctggccgt ccgacgagaa gaccgtgtcc ccggcgctga
     3661 agtggttccc ggtgaacgat tggctcattg gttgtcccct ttcaccctgt tcgataaatg
     3721 gaccctatca ctgcccgcaa gtacaccacg catgtgcact actccgcgag ttcggccggg
     3781 ccgattctcc cggcgccatg ggccggatgg ggataccggg catcccccgg aatcacccta
     3841 ccccgttcaa tctaaggtac agaaccaatt ggtggcagcg agcatgttga tcgctagtat
     3901 cgaattactg tcgggaagga aatggccgcc ggggcgtggc agcaggcagt aggagtcaga
     3961 gtagcgatgg acgatcaggt caaggacgaa gccgcggtgg cagagaccgg cgagcagccg
     4021 gccgccgaag ctggccagga acggtatgcg acgggtgtgg cgcggctcga caacatggtt
     4081 cacaagatcg acgacggagt gcgcaggatc tcgaaggaga ttcactcgct ggaagtcgaa
     4141 gaggatccaa atcgcgtcgc gaccggcatc aagccgctcg a
//
LOCUS       DATAOZ010000453         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9127298, whole genome shotgun sequence.
ACCESSION   DATAOZ010000453 DATAOZ010000000
VERSION     DATAOZ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9127298"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /gene="ftsY"
                     /locus_tag="VHR64_11585"
     CDS             <1..594
                     /gene="ftsY"
                     /locus_tag="VHR64_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX3303515.1"
                     /translation="VGKTTSIAKLAAYHQRFGRSVMLAAGDTFRAAAIDQLKVWGDRL
                     DVPVVAQAQGSDPGAVVFDAMQAAHNRNIDVLIVDTAGRLHNKSNLMAELSKIRKIMQ
                     RHVEDAPQEVLLVIDATTGQNGLVQAKEFAKSVDITDIMIAKLDGTAKGGIAFSVARE
                     LGTPISYIGTGEKAGDLAEFQPETYVDSLFFGEGDGL"
     gene            639..1967
                     /gene="ffh"
                     /locus_tag="VHR64_11590"
     CDS             639..1967
                     /gene="ffh"
                     /locus_tag="VHR64_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX3303516.1"
                     /translation="MFESLSDRLTETLGKLSRKGRLTEADVDDAMKEVRRALLEADVN
                     FRVVKDFVAAVRERAVGQDVLKSLTPAQTVIGIVNEELVRILGTEREPLRVPDRPPQI
                     LMLVGLQGSGKTTHAAKLAVHLRKQGRNPILVAGDVYRPAAVNQLQSLGKQVNISVYD
                     EGVQKNPVDIANNGVRYAIERGYNPVIIDTAGRLQIDEPMMQELVNVKGKVNPTEILL
                     VADAMTGQEAVSVAQSFHDRLGVTGLILTKMDGDARGGAALSIRSVTGVPIKFIGTGE
                     KVDALEPFFPDRLAQRILGMGDIQSLVDRAREQTTEEDAVKLQDKMLKGKFDLDDFLD
                     QLQKIKKMGPIGQLLEMIPGVGQQLRDAKSQVSDDDYKQIESIIQSMTPHERRNPERI
                     DFRRRKRIAAGAGRNPSDVGQLVKQFDEMQRMMKQFGKMAKKGKMPKGLM"
     gene            1986..2555
                     /locus_tag="VHR64_11595"
     CDS             1986..2555
                     /locus_tag="VHR64_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HEX3303517.1"
                     /translation="MAEAPTVTVYSTPTCPWCDRAKEYLRSRSVPFRDVDVSRDMDAA
                     REMIQLTGQQGVPVIATEHEVIVGFDQLRLARIADRFSGPKRPAFGVLGANAEDYFGR
                     HPEKKPSGEDDIKGVYVGQVKSNSVAQRAGIVVGDVIQAVANKRVRNMVALDQIMSSV
                     KAGDQVSARVLRGSDDVNVTLDFGTPSPA"
     gene            2562..>2990
                     /locus_tag="VHR64_11600"
     CDS             2562..>2990
                     /locus_tag="VHR64_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX3303518.1"
                     /translation="MTEWQALARVGLALVAGALLGLEREISDKPAGLRTYALVSQGAA
                     LFMVLSLMLSTGTHVQGTIVDPSRIASTVVQGIGFLAGGVIFTIGRHVKGLTTAAGVW
                     VAAAVGLLIGSGFYIIGFGGVVMTVLALRVFGSIAHRFPEG"
BASE COUNT          605 a          906 c          949 g          530 t
ORIGIN      
        1 gtcggcaaga cgacatcgat cgcgaaactt gccgcctacc accagcgctt cggccgttcg
       61 gtcatgctgg ccgcgggcga caccttccgt gccgccgcaa tcgatcagct gaaggtctgg
      121 ggcgatcgcc tggacgtccc ggtggtcgcg caggcgcagg gatcggatcc cggcgcggtc
      181 gtcttcgacg caatgcaggc cgcgcacaac cgcaatatcg acgtcctgat cgtcgacacc
      241 gctggccggc tgcacaacaa gtccaacctg atggctgagc tctccaaaat tcgcaagatc
      301 atgcaacggc acgtcgagga tgcgccgcag gaagtcctgc tggtcatcga cgcgaccacc
      361 ggccagaatg gcctggtcca ggccaaggag ttcgcgaagt cagtcgacat taccgatatc
      421 atgatcgcaa agctggatgg aacggcgaag ggcggaatcg ccttctcggt cgcgcgggag
      481 ctcggcacac cgatctcgta tatcggcacg ggtgaaaaag ccggtgatct cgcagaattc
      541 caaccagaaa cctacgtcga ctcgctcttt ttcggcgagg gcgacggatt ataggagcgg
      601 tattcgctgc tccgttgagc aattcgggaa cgtactgaat gtttgaatcc ctctccgacc
      661 gattgactga gacccttggc aagctctcgc gcaaggggcg tcttaccgag gcggacgtcg
      721 acgacgcgat gaaagaagtc cgccgggcgc tgctcgaggc ggatgtcaac ttccgggtcg
      781 tcaaggattt cgtggccgca gttcgggaac gggcagtcgg ccaggatgtc ctgaaatcgc
      841 tgacgccggc ccagaccgtg atcggcatcg tcaacgagga gctggtccgc atcctcggca
      901 ccgagcgcga gccgctgcgc gtgccggatc gcccaccgca aatcctgatg ctggtcggcc
      961 tgcagggatc cggtaagacg acgcacgccg ccaagctggc cgtccaccta cgcaagcagg
     1021 ggcggaaccc gatcctggtc gccggcgacg tttatcgccc ggctgcggtc aaccagctcc
     1081 agagcctggg caagcaggtc aatatatccg tctacgacga aggcgtgcag aagaatccgg
     1141 tcgacatcgc caacaacggt gtccgctacg ccattgagcg tggttacaac ccggtcatca
     1201 tcgacaccgc cggccgcttg caaatcgacg agccgatgat gcaggagctt gtcaacgtca
     1261 agggcaaggt caacccgacc gagatcctgc tcgtggccga cgccatgacc ggccaggaag
     1321 cggtctcggt cgcccagtcc ttccacgacc ggctcggcgt gaccggcctg atcctgacca
     1381 agatggacgg cgacgcccgg ggcggcgccg cactctcgat ccgctccgtc accggcgtgc
     1441 caatcaagtt catcggcacc ggcgagaagg tcgatgcgct ggagccgttc ttcccggacc
     1501 gcctcgcgca gcggatcttg ggcatgggcg acatccagtc cctggtcgat cgcgctcgtg
     1561 agcagacgac ggaagaggac gcggtcaagc tccaggacaa gatgctcaag ggcaagttcg
     1621 acctcgacga tttcctggat cagctccaga aaatcaagaa gatgggtccg atcgggcaac
     1681 tcctggagat gatcccggga gtcggacagc agctgagaga cgcgaagtcg caggtttcgg
     1741 acgacgacta caagcagatc gagtcgatca tccagtcgat gacaccgcac gaacgacgga
     1801 atcccgagcg aatcgacttc cggcgcagga agcggatcgc cgccggtgcc gggcgcaatc
     1861 cctccgatgt cgggcagctt gtgaaacagt tcgatgagat gcagcgaatg atgaagcaat
     1921 tcggcaaaat ggccaagaag ggcaagatgc cgaagggttt gatgtaacaa aaacaggagc
     1981 agaaaatggc agaggcacca acggtcaccg tgtatagcac tccgacctgc ccgtggtgtg
     2041 atcgcgccaa ggagtacttg cgatcacgca gtgttccgtt ccgcgacgtc gatgtctcca
     2101 gagacatgga tgccgctcgc gagatgattc agttgactgg ccagcagggc gtgccggtca
     2161 tcgccaccga gcacgaggtg atcgtcggct tcgatcagct gcggctcgcc cggatcgcgg
     2221 atcgattctc cggtcccaag cggccggcgt ttggcgtgct gggcgcaaat gccgaggact
     2281 acttcggccg gcacccggag aagaagccct ccggcgagga cgacatcaag ggcgtctacg
     2341 tgggacaggt gaaatcaaac agcgtggcgc agcgcgccgg gatcgtggtc ggcgatgtta
     2401 tccaggcagt cgccaacaag cgcgtccgga acatggtcgc gctcgaccag atcatgagct
     2461 cggtcaaggc cggtgaccag gtgagcgctc gcgtgcttcg cggttccgat gacgtcaatg
     2521 taacgctcga cttcggcacg ccaagcccgg cgtagtcttc gatgactgaa tggcaagcgc
     2581 tcgcccgcgt tggccttgcg ctcgttgccg gagcgctcct gggcctggaa cgcgaaatct
     2641 ctgacaagcc ggccggcctc cgcacctatg ccctggtcag ccagggcgcg gcgttgttca
     2701 tggtcctcag cctgatgctg tccactggaa cccatgttca gggcacaatt gtcgatccct
     2761 cgcgcatcgc atcgactgtt gttcagggca tcgggtttct ggcaggaggc gtgatcttca
     2821 cgatcgggcg ccatgtcaaa gggttgacca cggcggccgg agtttgggtt gccgccgccg
     2881 tcggattgct gattggcagc ggtttctaca tcatcggatt cggtggagtc gtgatgactg
     2941 tacttgcctt gcgcgtgttt gggtcaattg ctcaccggtt tccggaaggc
//
LOCUS       DATAOZ010000454         6715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7812818, whole genome shotgun sequence.
ACCESSION   DATAOZ010000454 DATAOZ010000000
VERSION     DATAOZ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7812818"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VHR64_11605"
     CDS             complement(<1..995)
                     /locus_tag="VHR64_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX3303519.1"
                     /translation="MRWPQLNMAAGPVEVSPRTLRDMARPVMYHYDPAFIELFAHASE
                     LMKQVYRTQYDVVIMQGEALLGLEAAAASLIRPGDKVLNLVSGVFGKWFEDFIERFGG
                     ETIEVRVPYNEAIDPEDVRKALNSNPGIKYLSAVHSETPSGTLNPIREIGKIAHEFGV
                     LTMVDTVSGLGSEMLSPEDWGMDIAIAGTQKCLSGPPGLALLSISPAAWEAMETTAKP
                     LRGSYLSILDWKTTWLEQRKFPYTPLVSEMYALESVLTQILEEGVERLVERHQQIAAA
                     CRTGVKALGLELWPAREEIAAPCVTAIRMPEGLTDSGIRTTMRERYGVMISPGYG"
     gene            complement(1013..2059)
                     /locus_tag="VHR64_11610"
     CDS             complement(1013..2059)
                     /locus_tag="VHR64_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEX3303520.1"
                     /translation="MVIRALLRPLFLLAILSLVLPSLVAAQGVDVKTVGMVVPASKTD
                     LGWNQQGADGLDAVAKELGITAKIQENGGYDDITPTLKDLKDDGAQLIICHASGYQTV
                     CPEFAAEEQVPVAVIENPKAVVPNLVSDIETQAQEVAYLAGVLAGRMTKTQTVGIVVS
                     GEPPTWNYMTVGFAEGLKASNGSAKLLYSVIGEDAYEDSAGAKRVTEQQLAAGADIIF
                     GMGDGASFGMIEAIRDFNKDHKDAPARFIDVIGDKSKDYSDVLLTSVLFDYAPTYKQM
                     IEDLKNNTFGKVYTLDVKNGGVRLLDLPDDVPQDVKDAVKKAQDDIVAGKIKVPAVGD
                     ADGVRSTLQQLGYR"
     gene            complement(2602..3012)
                     /locus_tag="VHR64_11615"
     CDS             complement(2602..3012)
                     /locus_tag="VHR64_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3303521.1"
                     /translation="MSPLAALTQTSPMTVAIVEDQAAIAELLHDVLEDAGYAPVIISS
                     PMTAVAEIAASGAQIVLLDIMMPRKNGWEVLDELRAEPVTRDLPVVLTSAVYDRIGRR
                     PLPFGGPIRFASKPFDVTELVDTIEGFRTRNSAG"
     gene            complement(3009..3527)
                     /locus_tag="VHR64_11620"
     CDS             complement(3009..3527)
                     /locus_tag="VHR64_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303522.1"
                     /translation="MNPADGIVPIADVPASVTTIRLASMIRDDSARILANWSMRIATL
                     PVFRALPELALDELQQDMPDLLDAILEAVSVSPYELDPTPMEEAGAKADAHGLKRAAS
                     FPVDVLLTEIQALQREVRNAIWRNAQEAPVSIVHELDERLNEVFELAERSAASAWVRR
                     HLDESIVTGDAA"
     gene            3853..5454
                     /locus_tag="VHR64_11625"
     CDS             3853..5454
                     /locus_tag="VHR64_11625"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3303523.1"
                     /translation="MTADLVVANAHIRTLDSDRPTATAVAITDGIITAVGDDAEVRPL
                     IGPQTEVIDGRDLAVVPGLTDSHFHPFWGAEATQGVDLTRAKTLDDLRTLLRAERQRI
                     GPDAWVTGWGLTFEMFQETGIRGDTFADAVDGGLAFLAFFDGHTAVASPAALAIADVT
                     GRETFPDFSTVVVDENGVPTGELREGGAMELVRRAMPVPTAEDKYRWYLEAMRKWNAV
                     GLTGIHAMDGSPATFELLREMEARGDLSIRMIVPLWQHPEYSFEQMREQLSCRDVRGE
                     LWRGGVAKFFIDGVIESGTGWLIEPDTKGMGTEPFWPDPAKYQEAVRIFAEAGFQCAT
                     HACGDMAVRCALDAYERAGAAEGIRHRIEHIEILSDVDLPRFAQLGVTASMQPLHMNA
                     FEPDGSDEWSLRAGPERRTQAWRTRELLNSGATLALGSDWMVAPYDPRIGMAWARARR
                     PAGMQGRGLINGEQALTGLEALAGYTTGAATAVSEEHLSGKIRVGYRGDLTAFAADPV
                     DTDPDDLVDLPVSLTVVNGRVVHRV"
     gene            5533..6573
                     /locus_tag="VHR64_11630"
     CDS             5533..6573
                     /locus_tag="VHR64_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX3303524.1"
                     /translation="MTTTDDILDIEQPGALVVYLRDQELIGRDEEPRVTVLSGGVSNR
                     TVLVEREFGEAWVVKQALAKLRVKVDWFSSPERIHREADGLRWLTEFAPPGSITPLAF
                     EDHDHHLLGMAAVPQPHENWKTMLLRGELDPDHIRQFATLIGRVHRESNRRLDELHPV
                     FGDRTFFETLRVEPYYRYTASQVPESDAFYAALIDDTYARQDTIVHGDFSPKNVLVHD
                     GRLILLDHEVIHLGDPGFDLGFGMTHLLSKAHHLSAQREDFREATLAFWETYWQTVNK
                     EGSAWTVGLEARAVRHTLGCLLARVRGRSPLEYLDDVARARQARVVNAIMSQPPGTVA
                     ELAAAFVAGIEE"
BASE COUNT         1116 a         2169 c         2263 g         1167 t
ORIGIN      
        1 tcgccgtagc cgggcgagat catcacgccg taccgctcgc gcatagtcgt ccggataccg
       61 gaatcggtca gaccctcggg catcctgatc gcggtcacgc agggcgcggc gatctcctcg
      121 cgagcaggcc agagctccag gcccagagcc ttgaccccgg tccggcaggc ggcggcgatc
      181 tgctgatgcc gctcaaccag ccgctcaacc ccctcctcga ggatctgggt caggaccgac
      241 tcgagcgcgt acatctcgga gacgagcggc gtgtagggaa atttgcgctg ctccagccaa
      301 gtcgtcttcc agtcgaggat cgagaggtag ctgccgcgca acggcttcgc ggtcgtctcc
      361 atcgcctccc acgcggccgg cgagatggag agcagggcca gaccgggcgg tccgctcaaa
      421 cacttctgcg tcccggcgat ggcgatgtcc atgccccagt cctcggggct gagcatctcg
      481 ctgccgagac cggagacggt gtcgaccatg gtcaggacgc cgaactcgtg cgcgatcttg
      541 ccgatctcgc ggatcgggtt gagcgtgccc gagggggtct cggaatggac ggccgagagg
      601 tacttgatgc ccgggttgga gttgagcgcc tttcggacat cctccgggtc gatcgcctcg
      661 ttgtagggga cgcgcacctc gatcgtctcg ccgccgaagc gttcgatgaa gtcctcgaac
      721 cacttgccga agacgccgga gacgaggttg agcaccttat cgcccggccg gatcaggctg
      781 gccgcggccg cctcgagccc gagcaacgcc tcgccctgca tgatcacaac gtcgtactgc
      841 gtccggtaga cctgcttcat aagctcagag gcgtgcgcga agagctcgat gaaggcggga
      901 tcgtagtggt acatgaccgg ccgagccatg tcccgcaagg tgcgcgggct gacctcgacc
      961 ggtccggcgg ccatgttgag ctgtggccag cgcatcggcg tgtccttcct gcttaccggt
     1021 agccgagctg ctggagggtg ctacgcacgc cgtccgcgtc gccgactgcc gggaccttga
     1081 tcttgccggc cacgatgtcg tcctgcgcct tcttgactgc gtccttgacg tcctgcggga
     1141 cgtcgtccgg caggtcgagc aggcggacac cgccgttctt gacgtcgagc gtatagacct
     1201 tgccgaacgt gttgttcttg aggtcctcga tcatctgctt gtaggtcggc gcgtagtcga
     1261 acaggaccga ggtcagcagg acgtcgctgt aatccttgga cttgtcgccg atcacgtcga
     1321 tgaagcgagc cggcgcgtcc ttgtggtcct tgttgaagtc gcggatggcc tcgatcatgc
     1381 cgaacgaggc gccgtcgccc ataccgaaga tgatgtcggc gccggcggcg agttgctgct
     1441 cggtgacgcg cttggccccg gccgagtcct cataggcgtc ctcgccgatc acgctgtaga
     1501 gcagcttggc gctgccgttg ctggccttca gcccttccgc gaagccgacc gtcatgtagt
     1561 tccaggtcgg cggctcgccc gagacgacga tcccaactgt ctgggtcttg gtcatccgcc
     1621 cggccagcac cccggccagg taagcgacct cctgggcctg cgtctcgata tcggagacga
     1681 ggttcggcac gaccgccttc gggttctcga ttaccgcgac cggcacttgc tcctcggcgg
     1741 cgaactccgg gcagaccgtc tggtagccgg aggcgtggca aatgatcagc tgcgcgccgt
     1801 cgtccttgag gtccttgagg gtcggcgtga tgtcgtcgta gccgccgttc tcctggatct
     1861 tggccgtgat gccgagctcc ttggcgactg cgtccagccc gtcggcgccc tgctgattcc
     1921 agccgagatc ggtcttgctg gccggcacga ccatcccgac cgtcttgacg tcgacgccct
     1981 gggccgcgac gagggagggg aggacgaggg agaggatcgc gaggaggaac agcgggcgca
     2041 acagcgcgcg aatgaccacg aaggtgtcct ttcgactgaa ccggccgcaa ggggatgacc
     2101 gggtctcgca tgatcagcgc aacgcatcgg aacccgatag gtaggtacgt gcggcgctca
     2161 ggtgaagact agcaatccgc cgatttgcct gccacttggt gggtggagag tggagggttg
     2221 agggtggagg gaacggaaag ggggtggatg gtggatggtg gttggaacgg gaaacgttgt
     2281 agtgtcgctt ggcaattcga cctgctgctg tggcgtgggt gccgctgtgc tctgccgcta
     2341 ccggtgggtc ggtggtgcct ggtgacgggt atcccgttac cccgtgccgg ccacaggggg
     2401 ccggggctac gttttggtgt gcggaccgtg gcagccggct tcagccgggt tcatgtctca
     2461 gcccgggtga cgccgggaag ccgcgtcttg caagcgagcg gaccgtcagg agtgccaagg
     2521 cgtgtacaga gccacaccac tacggccgtt tccacaacca atcaccaacc accaacaacc
     2581 cctacaccgt tccttcaacc ctcaacccgc cgaattgcgg gtgcggaagc cttcgatggt
     2641 gtcgaccagt tcggtgacgt cgaacggctt ggaggcgaag cggatcggcc cgccgaacgg
     2701 gagcggtcgc cgcccgatcc ggtcgtagac ggctgaggtc aggaccaccg gcagatcgcg
     2761 cgtgaccggt tcggcgcgga gctcgtccag cacctcccag ccattcttgc gcggcatcat
     2821 gatgtcgagc aggacgatct gcgcgcccga cgcggcaatc tccgctaccg cggtcatcgg
     2881 cgagctgatg atgaccggcg cgtacccggc atcctccagc acgtcgtgga gcagctcggc
     2941 gatcgccgcc tgatcctcga cgatcgccac ggtcatcgga gacgtttggg tgagcgcggc
     3001 aagtgggctc atgccgcgtc tcccgtcacg atcgactcgt ccaggtgtcg ccgaacccag
     3061 gcgctggcgg cgctccgctc ggccagctcg aagacctcgt tcagtcgctc atccagctcg
     3121 tgcacgatcg agaccggcgc ctcctgggca ttgcgccaga tcgcgttgcg gacctcccgc
     3181 tggagggcct ggatctcggt cagcaagaca tcgaccggga aactggccgc gcgcttcagc
     3241 ccgtgcgcgt cagccttggc gcctgcttct tccattgggg tcggatcaag ctcatacgga
     3301 gagacgctta ccgcttcaag gatcgcatcg agaaggtccg gcatatcctg ctgcagctcg
     3361 tccagggcga gctccggcag ggcccgaaac acgggcagcg tcgcgattcg catcgaccag
     3421 ttggccagga tgcgcgcgct gtcatcacgg atcatgcttg ccaggcgaat cgtcgtgacg
     3481 cttgccggaa cgtccgcaat cgggacaatt ccatcagccg gattcatctg tagagcctcc
     3541 ggttctcgat tgatcggtgt caggacacta ccagccatgt attcctctac cggtcttggc
     3601 gtacgggaca tgcgccaatc gcataccgtt gcatggtgca cagcgtacgc ccagccccgg
     3661 cgagacgtat ctacccgacg gcaggagttc gcatagcccg aatggcacac gcaccgtagg
     3721 cctgtcccgt acgttcacgt cctgattcct gtgcaaagga tgaacgcgaa tatcgagaaa
     3781 atgtgggatt tgccggagtt tgcgaagatt acgcacagcc catttggggc aggacatctc
     3841 aggagcttgc acgtgaccgc cgacctcgtt gtagccaatg cccacatccg cacccttgac
     3901 agcgatcggc cgaccgccac ggccgtcgcg atcacggacg gcatcatcac cgccgtcggg
     3961 gatgacgccg aggtccgccc gctgatcggc ccgcagaccg aggtcatcga cggccgggat
     4021 ctggcggtcg tccccggtct gaccgacagc catttccacc ccttctgggg cgccgaggcg
     4081 acccagggcg tcgacctgac cagggcgaag acgctcgatg acctccgcac actgctccgc
     4141 gcggaacggc agcggatcgg tccggacgcc tgggtgaccg gctggggcct gaccttcgag
     4201 atgttccagg agactggcat ccggggcgac accttcgccg acgcggtcga tgggggcctc
     4261 gccttcctcg ccttcttcga cgggcacact gccgtcgcct ccccggccgc gctcgccata
     4321 gctgatgtga ccggccgtga gaccttcccg gacttttcga cggtggtggt cgacgagaac
     4381 ggcgtgccga ccggagagct gcgcgagggc ggtgcgatgg agttggtccg gcgggcgatg
     4441 ccggtcccga ccgccgagga caagtaccgc tggtacctcg aagcgatgcg gaagtggaac
     4501 gcggtcggcc tgaccgggat ccacgcgatg gacggctcgc cggcgacctt cgagctgctg
     4561 cgcgagatgg aggcccgggg cgacctgagc atccggatga tcgtcccgct ctggcagcat
     4621 cccgagtaca gcttcgagca gatgcgtgag cagctgtcct gccgggacgt ccgcggcgag
     4681 ctctggcgcg gcggcgtggc caagttcttc atcgacgggg tgatcgaatc cggcaccggc
     4741 tggctgatcg agccggacac caaggggatg ggcaccgagc cgttctggcc ggatcccgcc
     4801 aagtaccagg aggcggtccg catcttcgcc gaggccgggt tccagtgcgc gacccacgcc
     4861 tgcggcgata tggccgtccg ttgcgcactc gatgcctatg agcgagcagg cgcggcggag
     4921 ggcattcgtc accggatcga acacattgag atcctgtcgg atgtcgatct gccccggttc
     4981 gcacagctcg gcgtgaccgc ctcgatgcag ccgcttcata tgaacgcctt cgagccggat
     5041 ggcagcgacg agtggagcct ccgcgccggt ccggagcggc gcacgcaggc atggcgaacg
     5101 cgcgagttgc tcaacagcgg cgcaacgctc gcgctcggct ccgactggat ggtcgcgccg
     5161 tacgacccgc ggatcggcat ggcctgggcg cgcgctcgcc ggccggccgg gatgcagggg
     5221 cgcggtctga tcaacggcga gcaggcgctg acgggactcg aggcgctggc cggctacacg
     5281 accggcgcgg ccacggcggt ctccgaagag cacctgagcg gcaagatcag ggtcggctac
     5341 cggggcgatc tgaccgcctt cgctgccgat ccggtcgaca ccgacccgga cgacctggtc
     5401 gatttgccgg tgtcgttgac ggtggtcaat ggtcgggtgg tgcatcgggt ctgaggcgag
     5461 aagtcgagca ggcgtagagg cagatggcga tctgcccgca actcatcgcg accggggaag
     5521 ccaacgaaac cgatgacgac aactgacgat atcctcgaca tcgagcaacc cggcgcgctg
     5581 gttgtttacc tccgcgatca ggagttgatt ggccgcgatg aggagccgcg cgtgacagtg
     5641 ctctccgggg gggtctcgaa ccggacggtg ctggtcgagc gcgagttcgg tgaggcgtgg
     5701 gtcgtcaagc aggcgctggc caagcttcga gtcaaagtcg attggttcag cagcccggag
     5761 cgcattcacc gcgaggccga cggtctgcgc tggctgacgg agttcgcacc gccgggcagc
     5821 attacgccgc tcgccttcga ggatcacgac catcatctgc tcggcatggc cgccgtcccg
     5881 cagccgcacg aaaactggaa gacgatgctg ctgcggggcg agctcgatcc cgatcacatc
     5941 cgccagttcg cgacgctgat cggccgggtc caccgcgaga gcaaccggcg gctggacgag
     6001 ctgcatccgg ttttcggcga ccgcaccttc ttcgagacgc tccgggtcga gccctattac
     6061 cggtacaccg cgagccaggt tccggagtcg gatgcgttct acgccgcgct gatcgacgac
     6121 acgtatgcgc gccaggatac gatcgtccac ggcgacttca gccccaagaa tgtgctcgtt
     6181 catgacggcc ggctgatcct gctcgaccac gaagtgatcc accttggcga ccccgggttc
     6241 gacctcggct tcggcatgac tcatctgctc agcaaggcgc accacctgtc cgcgcagcgg
     6301 gaagatttcc gggaagcgac gcttgcgttc tgggagacct actggcagac cgtaaataag
     6361 gaaggctccg cttggaccgt cggtctcgaa gctcgcgcgg tgcggcatac cctcggctgc
     6421 ctcctggccc gggtgcgcgg ccgctctccg ctcgagtacc tggacgatgt cgctcgcgcc
     6481 cggcaggctc gcgtcgtcaa tgcgatcatg tcgcaacccc cagggaccgt ggcggagctg
     6541 gccgcggcat ttgtcgcggg catcgaggag tagcatggct gaaagcgggc agattgcgcg
     6601 gctgcatgcg gtcgagatcc tggactcccg cggtcggccg acggtccaga cgacgtgcga
     6661 gctgaaggac ggcacgcagg cgacggtgtc agtcccgtcc ggcgcatcaa ccggc
//
LOCUS       DATAOZ010000455         4012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4127380, whole genome shotgun sequence.
ACCESSION   DATAOZ010000455 DATAOZ010000000
VERSION     DATAOZ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4127380"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..589)
                     /locus_tag="VHR64_11635"
     CDS             complement(245..589)
                     /locus_tag="VHR64_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303525.1"
                     /translation="MSGPTRRSIITLPFDQRAIPEIALLLEATATLAPFQLPGGEVHQ
                     LTVQRAEAPPTVLLTLWPTIRRVDAISQSATVVFTSVTSVDLVEGVEVLFRRGGGEYL
                     IVTVAGKVIVRA"
     gene            804..1409
                     /locus_tag="VHR64_11640"
     CDS             804..1409
                     /locus_tag="VHR64_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303526.1"
                     /translation="MFQRGNATLPDHGDRLARREGTRLWYRVEPGTQHEDHLVWEGHG
                     REAPAAAAEETPPRTRTGQLRSMEALQATLEQAETPAPARQNVDDIRTLTDWRDSPRH
                     CPVLLLSSTTLLDARQPGPLLGPGTKYRIVTVNDGVVCLQVEALDGQIGLGYCNAVDL
                     MCYEPEIMYLRPEKRTRRQTARLSLSRLSHRITGGTGSLGG"
     gene            1533..2807
                     /locus_tag="VHR64_11645"
     CDS             1533..2807
                     /locus_tag="VHR64_11645"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303527.1"
                     /translation="MIATLRQRNFALLWTAGLISLIGDWAFYAAMPIFILDRTGSAFQ
                     SGLVWAAIALPGIFIGPFAGVYVDRLDRRRIMLFGNVAQAIAATVLLVGGESFGIWLA
                     MAVLLVQSSLYAIYSPAESALLPTVVDEEHLVTANALNSLNDNIARIAGPLIGALVYA
                     WFGIRGIALANMASFLGSAVLVGMVSLAVAARSQSGIDVAATSESIWRSMRTGASVVW
                     HSSVLRSLFMIELLIVLADGPLTAMLAPFVRQTLDRTNADFGVLLSVRGVAGVAGGFV
                     IAHVAHKFRNDRMMGACLLVIGVGVVAIAIIQNYPMTLAIMVLGGPAIVGMNTTFITM
                     LQRNSEDRVRGRIFALLGAFSGVIFLISTVIGSVATKVMSPPAIMLISGLTYTAAGVG
                     ALLLLANRRTAQPVEDFVKQDQTGMDATAPTA"
     gene            2885..3907
                     /locus_tag="VHR64_11650"
     CDS             2885..3907
                     /locus_tag="VHR64_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX3303528.1"
                     /translation="MTLTTQPISEPDGLPGPTTFDLNTLPVSIRDIWAAKLRISSMIW
                     RTSLLPTATLSRMTNTNLRIKTENLQRTCSFKFRGALNAVLQLTPEQRAKGVCTFSAG
                     NHGQGLAYAAQLSGVHCTVFMARDANPLKIEAIRGYGADLVFGDTIGDASDRMEVFQL
                     ETGATYVSPYGDPAVIAGQGTIGLELLEDFPELEYVIVGIGGGGLASGLALAVKSIRP
                     DIKVIGVEPEGAPTLTRSLEEGRPVRLESIKTIADGLGAPYALPIPFEVIRAMLDDVV
                     LVDEDSLAEAVRLLLFRCKLLVEPAGAASVAALLTRKVKVPEGANTVAILSGGNIDPE
                     RLKSLL"
BASE COUNT          676 a         1408 c         1226 g          702 t
ORIGIN      
        1 cggccccctg tggccggcct gggttcaacg gcatgcgcgg tcgatgaagc ctaaccggca
       61 ccctcccccg gacgcacccg acgccgaggc gagccgcgcc ccgacagaag catgccgtca
      121 gcccctttag tgggcttatc gtgattcagc ccgggccttt agccccggcc ggagccgtgc
      181 ggttggcttc cgatggaaag gtgggttccg accgggtgcc atgagggccg cggtcttttc
      241 ccgctcacgc ccgcacgatc accttccccg caaccgttac gatcaggtac tcgccaccac
      301 cgcgccggaa cagcacctcg accccctcga ccagatccac cgacgtcacg ctcgtgaaga
      361 cgaccgtcgc cgattggctg atcgcgtcga cgcgccggat cgtcggccag agcgtcagca
      421 gcaccgtcgg cggagcctcc gcccgctgca ccgtcaactg gtggacctcg ccacccggga
      481 gctgaaacgg cgccagggtc gccgtcgcct cgagcagcag ggcaatctcc gggatcgctc
      541 gctggtcgaa tggcaacgtg atgatcgatc gccgagttgg cccggacacc gggtcatgcc
      601 tgtccatcgc gcaagtatgg cgcggacgca gccggtacct tggtcccgac tggagtagga
      661 ccgtggggtc cctccaaatg gccaaccggc ccattactat cgggctatga cagatgggtg
      721 atcgatacag gaccgcagcg ccagaccggc gcacgtttac ctcgtagccg ccacatgtat
      781 tgaagatggg ggagttggac gagatgttcc agcgtggaaa tgcgacgttg cctgatcatg
      841 gcgatcggtt ggccaggcgg gaaggcacga ggctttggta ccgggtcgag ccgggcacgc
      901 agcacgagga tcacctggtc tgggaagggc acggccgcga ggctcccgcc gccgccgccg
      961 aagagacgcc accccgcacc cggaccggcc agctccgctc catggaagcc cttcaggcca
     1021 ccctcgagca ggcggaaacg cccgcgccgg ctcgccagaa cgtcgacgac atccgcaccc
     1081 tgactgactg gcgcgattca ccgcgacact gcccggttct gctgctctcg tcgacgacat
     1141 tgctcgacgc ccgccaacca ggccctctgc tcggcccggg cacgaagtac cggatcgtga
     1201 cggtcaacga cggggtcgtc tgtctccagg tcgaggcact ggatggccag atcggtctcg
     1261 gatactgcaa tgccgtcgac ctcatgtgct acgagcccga aatcatgtac ttgcggcccg
     1321 agaaacgaac ccgccgccag accgcccgcc tgagcctgag ccggctctcc caccgcatca
     1381 ccggcgggac cggatcgctc ggcggataac acctgccaac cagccccttc tgccaccaac
     1441 ggcagcgact gcgctctccc ccggcgcacc gctgccgttg gtgtgtctga cggtagactt
     1501 cccacctgca tgtcaagact gccaggatcg ggatgatcgc cacccttcgc caacggaatt
     1561 tcgcgctact ctggaccgcc gggctgatct cgctgatcgg cgattgggcc ttctacgcgg
     1621 ccatgccgat cttcatcctc gatcggaccg gctccgcctt ccagtccggc ctcgtctggg
     1681 cggcgatcgc gctgcccggc atcttcattg gcccgtttgc cggggtctac gtcgatcggc
     1741 tggaccggcg ccggatcatg ctcttcggca acgtcgccca ggccatcgcc gcgaccgtgc
     1801 tcctggtcgg aggcgagtca ttcgggatct ggctggcgat ggcggttctg ctcgtccagt
     1861 ccagcctcta cgccatctac tcgccggccg agagcgcgct cctgccgacc gtggtcgacg
     1921 aggagcattt ggtcacggcc aacgcgctca attcgctcaa cgacaacatc gcccgcatcg
     1981 ccgggccatt gatcggcgct ctggtctacg cctggttcgg cattcgcggc atcgcgctcg
     2041 ccaatatggc ctcgttcctt gggtccgcgg tactggtcgg catggtttcg ctggcagtcg
     2101 cagcgcggtc ccaatcaggc atcgacgtgg cggcaacatc cgagtcgatc tggcgatcga
     2161 tgcgcaccgg tgcaagtgtc gtctggcaca gctccgtgct gcggtcgctc ttcatgatcg
     2221 aattgttgat tgtcctcgcc gacggcccct tgaccgccat gctcgcgccc ttcgtccgcc
     2281 agacgctcga ccgcaccaac gccgattttg gcgtcttgct ctcggtgcgc ggcgttgccg
     2341 gggttgccgg tgggttcgtg atcgcgcacg tcgcgcacaa gttccgcaac gaccggatga
     2401 tgggtgcctg cctcctggtg atcggcgtcg gcgtggtggc gatcgcgatc atccagaact
     2461 acccgatgac cctggcgatc atggtcctcg gaggaccagc cattgtcggt atgaacacga
     2521 cgttcataac catgctccag cgcaacagcg aggatcgcgt ccggggccgg attttcgccc
     2581 tcctcggcgc cttcagcggt gtcatcttcc tgatcagtac agtgatcggc agtgttgcca
     2641 cgaaggtcat gtcgccgccg gccatcatgc tgatcagcgg cctgacctac accgcggccg
     2701 gagtcggggc gctcctgctc cttgccaatc gccgcaccgc tcagccggtc gaagatttcg
     2761 tcaaacagga ccaaacagga atggacgcaa cggcccccac ggcttgatca tgaatcaccc
     2821 gccttatact cgaaatcgga cataacgttc gcagcgaaca cgccaacagc cggggaccga
     2881 cgccgtgaca ctgaccaccc agcccatttc cgaaccggac ggtctgcccg gaccgaccac
     2941 cttcgacctc aacactctgc cggtctcgat ccgggatatc tgggcagcca agctgcgaat
     3001 ctcttcaatg atctggcgga cgtcgctcct gcccacggcc accctcagcc ggatgacgaa
     3061 caccaacctt cggatcaaga ccgagaacct gcagcggacc tgctcgttca agttccgcgg
     3121 cgcgctcaac gcagtcctgc aactcactcc ggagcagcgg gccaagggcg tctgcacctt
     3181 ctcggccggc aaccacggcc aggggctggc ttacgccgcg cagctctcgg gcgtccactg
     3241 caccgtcttc atggcccgcg atgccaatcc gctcaagatc gaggccatcc gcggctacgg
     3301 cgccgacctc gtcttcggcg atacgatcgg tgacgcctcc gaccggatgg aggtcttcca
     3361 gctggagacc ggcgcgacct acgtctcgcc ctatggcgat ccggccgtaa tcgccgggca
     3421 gggcacgatc ggtctcgagt tgctcgagga cttcccggag ctggagtacg tgatcgtcgg
     3481 gatcggcggt ggcgggctcg cctcgggcct ggccttggcc gtcaaatcga tccggccgga
     3541 catcaaggtc atcggcgttg agcccgaggg cgcgccgacc ctgacccgct cgctcgaaga
     3601 gggacgacca gtccgactgg agtcgatcaa gacgatcgcc gacggtctcg gtgcacccta
     3661 tgccttgccg atcccgttcg aggtgatccg ggccatgctc gacgacgtcg tgctggtcga
     3721 cgaggattcc ctggccgagg cggtccgtct cctgctcttc cgctgcaagt tgctggtcga
     3781 gccggccggg gcagcgtcgg tcgccgcgct gctgacccgc aaggtcaagg tccccgaagg
     3841 cgccaacacc gtcgccatcc tcagcggcgg caacatcgac cccgaacgcc tcaagagcct
     3901 gctctaaggt agtcaccgat tccttagagt ggcgcgggga tgctctcttc ccttgagggg
     3961 agagccagaa aggggaccac gccccgtatc ggtgaacccc aaggctggca ga
//
LOCUS       DATAOZ010000456         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1234368, whole genome shotgun sequence.
ACCESSION   DATAOZ010000456 DATAOZ010000000
VERSION     DATAOZ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234368"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..534
                     /locus_tag="VHR64_11655"
     CDS             325..534
                     /locus_tag="VHR64_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX3303529.1"
                     /translation="MSVYRVIDVVGTSTTSWEEAAADAIKTAGHSVRDLRVGEVVKQD
                     IHIGDGGELIYRTKIQLSFKYEHES"
     gene            complement(633..959)
                     /locus_tag="VHR64_11660"
     CDS             complement(633..959)
                     /locus_tag="VHR64_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX3303530.1"
                     /translation="MVTPDDIRTVAASLPRTEEALVRDQVKFRVGRIVYVALSRDELS
                     MGFGFPKDERAALVAAHPEKFFMPIQSDLRYNWVECGWTRSTRQRCGNWSSTRGAWSC
                     ELPPNI"
     gene            complement(1051..>1619)
                     /locus_tag="VHR64_11665"
     CDS             complement(1051..>1619)
                     /locus_tag="VHR64_11665"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3303531.1"
                     /translation="REHRSDAEPLHPDLESFLAAGEPPIYIGFGSSVGPDPAHLGAVV
                     GDAVQQAGIRAVIASGWGALRGVESNSNTMVIESAPHRSLFPRVASVVHHGGAGTTAA
                     GLLAARPTIICPFQGDQHFWGAAVHRARAGPPPLPAKKLAPERLASVIRKAGDDGDMR
                     TRAIELSKRIAEEDGTGRAAEQIESILA"
BASE COUNT          290 a          532 c          510 g          287 t
ORIGIN      
        1 gcccgccgcc gtacacgatc ttccgaatcg tcccgtcgaa ggtgttcggc ctccccggca
       61 tgtacgggat ggatcagttc gatcaggcag atctgccgcg gccgacgcgc tgggacttcg
      121 gcagaggctg acctcgaggg gcctcgcgcc agcgttcctc ggctgactgt ccccacatcg
      181 ccctgccagg tggcgcatat tcatcagctt gatcttcagg gcgccacgcc gatcgggatg
      241 cgctggttgc tctcgggccc ctgagtgcga gatcatcacc gtgccgaatc gggccgatcg
      301 gcacatccaa ctaggagggc aactatgagc gtctaccggg tgatcgacgt tgtcggcacg
      361 agcacgacct cctgggagga ggctgctgct gacgcgatca aaactgcggg ccactcggtc
      421 cgggaccttc gggtcggcga ggtcgtcaag caggacattc acatcggcga cggcggcgag
      481 ctcatctacc gcacgaagat ccagctgtcc ttcaaatacg agcacgaatc gtagggcgcg
      541 tcaggcgcgc ctcatcgctt gggttgcatc ggtagtcatc atcgcagaca ccgaagccgc
      601 ccgcgtctgc atggtcgctc acggatgatc acctagatat tcggcggcaa ttcgcacgac
      661 catgcgccac gcgtcgacga ccagttcccg catctctgtc tggtcgatcg cgtccagcca
      721 cactcgaccc agttgtaacg caggtccgac tgaatcggca tgaagaactt ctctggatga
      781 gcggcgacca gtgcggcgcg ctcgtccttc ggaaagccga agcccatcga cagctcgtct
      841 cggctgagtg ccacatagac gatccggccc acccgaaact tgacctgatc ccggaccagc
      901 gcctcttcgg tccgcggcaa cgacgccgca accgtccgga tgtcatctgg ggtcaccatt
      961 gcgctgctac ctcttggcag tacacggatg gcaagaggta gtctggcgcg agtcacgagc
     1021 gccgactctc gaactggaaa ccggctgagg ctaagccaaa atgctttcga tctgttcggc
     1081 ggcccggccc gtaccgtctt cctcggcgat ccgcttcgac agctcgatgg cgcgagtccg
     1141 catgtcgcca tcatcgccgg ctttccggat caccgaagca agtcgctcgg gggcgagctt
     1201 cttggcaggc aatggcgggg gcccggcgcg ggcgcggtga accgcagctc cccagaaatg
     1261 ctggtcgcct tggaaggggc aaatgatcgt tggacgtgct gcgagcagac cggcagctgt
     1321 cgtccccgcg cctccgtgat gaacgacgga ggcaacgcgg gggaacaacg aacgatgggg
     1381 tgcgctttcg atcaccatgg tgttggagtt gctctcgaca ccgcgcagcg ctccccagcc
     1441 gctcgcgatc acggcacgga tgcccgcctg ttgtactgca tcgcccacca cagcgcccag
     1501 atgggcggga tcggggccga cactgctgcc gaagccgatg tagatcggcg gctcgcccgc
     1561 ggccaggaag ctctccaggt cgggatgcag cggttccgcg tcggatcggt gctcgcgca
//
LOCUS       DATAOZ010000457         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4128788, whole genome shotgun sequence.
ACCESSION   DATAOZ010000457 DATAOZ010000000
VERSION     DATAOZ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4128788"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VHR64_11670"
     CDS             complement(<1..630)
                     /locus_tag="VHR64_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3303532.1"
                     /translation="MKTTRSGQPLLEVKDLRTQFMTQDGVVKAVDGVSFMVMPGETLG
                     VVGESGSGKSMTGLSIMRLIPMPPGKIASGEVLLEGRDILKMSDNDVRKIRGKEIAMI
                     FQDPMTSLNPVLTISRQLCEAIQLHLGMNKDQARARAVDLLKMVGIPNAEKRLDQYPH
                     QFSGGMRQRVMIAMALSCNPKLIIADEPTTALDVTIQAQILDLMRTLQSE"
     gene            895..1977
                     /gene="dprA"
                     /locus_tag="VHR64_11675"
     CDS             895..1977
                     /gene="dprA"
                     /locus_tag="VHR64_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX3303533.1"
                     /translation="MPSSDARYWVGFQHVRRIGPVRLKRLIDRFGSISAAWNASQAEL
                     RTVLDDASVESIARVRSSLDLDREMERIDRLGIEVLTLGDTTYPHLLREVPNPPAVLY
                     VKGAICDGDFKAVGMVGTRRCTAYGRQMAFTMAEELANAGVTVVSGLALGIDGQAHRG
                     ALAGGGRTIAVLGSGVDQIYPSSHRDLATQIIENGAIISDYPPGTKPDARNFPPRNRI
                     IAGLSRGIVVVEAPSRSGALITVDFAADYGRDVFCVPGNAMSENSAGCHAVIRDGGRL
                     VTSAVDVLDDLGMSVPAPGAAVQQSFPMSEEERHLFNYIRWEPQHIDEIAVGAGFTSS
                     QAGALLTLLELKGAVRDAGGQHYVRS"
     gene            2185..>2573
                     /locus_tag="VHR64_11680"
     CDS             2185..>2573
                     /locus_tag="VHR64_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX3303534.1"
                     /translation="MKSNGRLVIVESPAKARTIERYLGSGYSVTASMGHIRDLPKSTM
                     GVDLEHDFTPKYTIPRDKSKLVKELKESVDHANELILATDPDREGEAIAWHLVAATNP
                     QDKPVKRVVFNEITPDAVRAAMENPRE"
BASE COUNT          484 a          786 c          810 g          493 t
ORIGIN      
        1 ctcgctttgc agcgtccgca tcaggtccag gatctgggcc tggatcgtca cgtcgagcgc
       61 agtcgtcggc tcgtcggcga tgatcagttt cgggttgcag gagagcgcca tggcgatcat
      121 gacgcgctgg cgcataccac cggaaaactg gtgcgggtac tggtcaagcc gcttctccgc
      181 gttcggaatc ccgaccatct tgagcaagtc gaccgcgcgg gcgcgagcct gatccttgtt
      241 catgccaagg tggagctgaa tcgcctcgca gagctgtcga ctgatcgtca ataccgggtt
      301 gagcgaggtc atcgggtcct ggaagatcat ggcgatttcc ttgccacgga tcttgcggac
      361 atcgttgtcg gacatcttga ggatgtcgcg gccctcgagc aacacctcac cgctggcgat
      421 cttgcccggt ggcatcggaa tcaggcgcat gatcgagaga ccggtcatgc tcttgccgct
      481 tccggactcg cccacgacgc cgagcgtttc gcccggcatc accatgaacg aaacaccgtc
      541 aactgccttg acgacgccgt cctgggtcat gaactgtgtt ctgaggtctt tgacctcgag
      601 gagcggttgc ccacttcgag tggttttcac ccgagacgct ggtgctactt ctgcgaccat
      661 cgttcattca atccttaagt ctgttggacg gtcccgcccc tgttcgccgc aactcgacct
      721 cgagcgcaga agcttacaga ttgctcagca aagcgcccta ccgaacctcg cagaacgctt
      781 cgttggaatc gtgttcggca ggatagcata cggtaggagg cgcaaccaac gactcctgtg
      841 accgtctgcg cgcccggcgc agataccgaa tattatgctg tgtgagtgta cataatgcca
      901 tcgtccgacg cccggtattg ggttggattt cagcatgtcc ggcgtattgg tccagtccgg
      961 ctcaagcggc tgatcgatcg gttcggatcg atcagcgccg cctggaacgc atcgcaagcc
     1021 gaattgcgga cggttctgga cgacgcatcg gtcgagagca ttgcccgggt tcgttcctcg
     1081 ctcgatctgg atcgcgagat ggagcggatc gatcgtctcg gcatcgaggt gctgacgctc
     1141 ggggacacga cctatccgca cctgctgcgc gaggtgccaa atccgccggc cgtgctctac
     1201 gtgaaaggcg cgatctgcga cggcgacttc aaggcggtcg gtatggtcgg tacgcggcgc
     1261 tgcacggcgt acggccggca gatggccttt acgatggccg aagagctggc gaacgccggc
     1321 gtgacggtcg tttccgggct ggcgctaggg atcgacggtc aggctcaccg gggtgcgttg
     1381 gcaggtggtg gccgcacgat cgcggtcctc ggttcgggtg tcgaccagat ctacccgtcg
     1441 agtcatcgtg atctggcgac gcagatcatc gagaacgggg cgatcatctc cgattacccg
     1501 ccggggacca agcccgatgc gcgcaatttc ccaccgcgca accgaatcat cgctggcctg
     1561 agtcgcggca tcgtggtcgt tgaggcgcct tcccgcagcg gggcgctgat taccgtcgat
     1621 ttcgcggcgg actacggtcg agacgtcttc tgcgttccgg gcaacgccat gagcgagaac
     1681 agcgccgggt gccatgccgt aatccgggac ggtggccggc tcgtgaccag cgcagtcgat
     1741 gtcctggacg acctcggaat gtcggttccg gcgcccggcg cggcggtcca gcagtcgttc
     1801 cccatgtcgg aggaagaacg gcacctcttc aattacatac ggtgggagcc gcagcacatc
     1861 gatgagatcg ccgtcggagc cggctttacc tcatcccagg ccggcgcctt gctgaccctg
     1921 ctggagctga agggcgcggt gcgcgacgcc ggtggccagc attacgttcg gtcgtgatgt
     1981 acgcttcgcc gtcttgacag cccttgacac cattcgtagg gttgaaaaga tattcgcggt
     2041 tcggccgtct gcgccggacc ctctcgttcc ggtcatagat aatgaataga gacgttacgt
     2101 atgccgactt ctaaaaccgg tagttccggc acgcgaacaa gcaagaaatc caccaccaag
     2161 accaagcgca gcacagcgtc gagggtgaag tcgaacggtc ggctcgtcat cgtcgagtcg
     2221 ccggccaagg cccggacgat cgagcgatac ctcggcagcg gctactccgt gaccgcgtcg
     2281 atgggccaca tccgggacct gcccaagagc acgatgggcg tggacctgga gcatgatttc
     2341 acgcccaagt acacaatccc acgggacaag tccaagctcg tcaaggagct caaggaatca
     2401 gtcgatcacg cgaacgagct gatcctggcg acagacccag accgtgaagg cgaggcgatc
     2461 gcctggcacc tggtcgcggc gacaaacccg caggacaagc cggtcaagcg cgtcgttttc
     2521 aacgagatca cgccggacgc ggtccgcgcg gccatggaga atccgcgcga gat
//
LOCUS       DATAOZ010000458         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2292798, whole genome shotgun sequence.
ACCESSION   DATAOZ010000458 DATAOZ010000000
VERSION     DATAOZ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292798"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VHR64_11685"
     CDS             <1..411
                     /locus_tag="VHR64_11685"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX3303535.1"
                     /translation="VEPARWIAANIHAGNRTVTFEELRPELGLLARIADDQLTFKQGE
                     PDHSEQAVTVVAADVVATMPLAGMVDLDVERARLEKELEAAQQEQERATASLNNEAFV
                     SRAPARVVEQQRNRLRIANEQIEVLTKRLAMFNG"
     gene            408..1046
                     /locus_tag="VHR64_11690"
     CDS             408..1046
                     /locus_tag="VHR64_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX3303536.1"
                     /translation="MSDWHPGDRLDLAWYERPTEIIARELLGMVLVTNEPGGLTAGRI
                     VETEAYLGDRDAASHANLYKRGRAALIRRAGHVYMYRSYGIHTIFNIVTEAEGTAGAV
                     LIRAIEPVAGIELMIARRGADRISQLGKGPGNLAMALGLRLDDDNRDLVTDDHIYLTT
                     GNPPKEIAVSTRIGITRNTDALLRFFDPDSASVSSHRRAAIIEPATGRQGRE"
     gene            1100..2824
                     /gene="mutL"
                     /locus_tag="VHR64_11695"
     CDS             1100..2824
                     /gene="mutL"
                     /locus_tag="VHR64_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX3303537.1"
                     /translation="MPIKLLTPETIGKIAAGEVVERPSSVVKELIENALDAGARRISV
                     DVRGGGIESIEISDDGIGISPDDLSMALERHATSKLRSFDDLDTLATLGFRGEALASI
                     SAVSSFAMRSRTEDRPAGTSVEVEFGDRHDSKTVAAPVGTTVCVRDLFGNVPARRKFL
                     RQPSTEHGYVGRVVAAYACAYPHVQFSLTLDGRNVISTNGSGDHLAAASGVFGVEVGK
                     AALLVAPLEDSAAVPGVRVDGWIGAPTVSRSHRQDMIFFVNGRWVQHRSMSFALEEAY
                     HSLLMVGRHPIAFLHVHVDPASIDVNVHPTKAEIKFQDERSACRAVQRSAHAALAQSP
                     HDDLPGASFSPVFAPVHQPAFAMPMPAGRVQAIEPENQIGAPAPSAAHQSGVPMLRVL
                     GQVGGSYIVAEGPDGMYLIDQHAAHERVMYEKIASQMRTAGVDRQQFLDPLLVELPPE
                     EHAVFEKSRDDLAAIGFEIEPFGEAIVAVRSVPALVKGVDVPERLHLILQELADGGAG
                     ESWFDSVAISAACHTAIRAGQTLSLAEMRELVNDLERTSQPRACGHGRPTMLHMSQFD
                     LEKQFSRR"
BASE COUNT          528 a          843 c          956 g          550 t
ORIGIN      
        1 gttgagccgg cgcgctggat cgcggccaat atccatgccg gtaaccgcac cgtcacgttc
       61 gaggaactgc gacccgagct tgggttgctg gcgcgcattg ccgacgacca gctcacgttc
      121 aagcagggtg agcctgacca ctccgagcag gcggtgacgg tggtcgccgc cgacgtcgtg
      181 gcaacgatgc cgctggcggg catggtcgat ctcgatgtcg agcgcgcccg tctcgaaaag
      241 gagctggaag cggcgcagca ggaacaggag cgggcgacag cctccttgaa taacgaggcg
      301 ttcgtctctc gcgcgcctgc ccgggtggtt gaacagcagc ggaaccgtct gcgcattgcg
      361 aacgagcaaa tcgaggtatt gaccaagcgg ttggcgatgt tcaacggatg agtgattggc
      421 atcccggaga tcggctggat cttgcctggt acgagcgtcc gactgagatc attgcgcggg
      481 aactgctcgg catggttctg gtgacgaacg agcccggtgg gttgaccgcg gggcggatcg
      541 tcgaaaccga ggcctatctc ggtgaccggg atgccgcgtc acatgccaat ctctacaagc
      601 gtggccgggc ggcgctgata cgccgggctg gccacgtcta catgtaccgc tcgtacggaa
      661 tccacacgat cttcaatatc gtgacggaag ccgaaggtac ggctggtgcg gtgctgatca
      721 gggcaatcga gccggtcgca ggtattgaac tgatgatcgc gcggcgagga gccgacagga
      781 tctcgcaact tggcaaaggg ccgggcaatc tcgccatggc gcttgggttg cgcctcgacg
      841 atgacaatcg cgatctggtc actgatgacc acatctatct gacgacgggg aacccgccca
      901 aggaaatcgc agtctcgacc cggatcggca tcacgaggaa taccgacgca ctgctacgct
      961 ttttcgatcc cgacagcgcg tctgtgtcgt cccatcgtcg cgcggccatc atcgagccgg
     1021 ctaccgggcg ccagggccga gagtagtacg attgttgctg aggatgttcg tacagccata
     1081 aggtcgagat tggtttacga tgccgattaa actcctgaca cctgaaacga tcggaaagat
     1141 cgctgcgggc gaggtcgtcg agcgaccctc gtcggtcgtg aaagaactga tcgagaacgc
     1201 gctcgacgcc ggagcgcgtc gcatttcggt cgatgtgcgg ggcggcggca tcgaatcgat
     1261 cgagatctcg gatgacggca tcggcatctc gcctgatgac ctctcgatgg cactggaacg
     1321 gcatgccacc tcgaagttgc gaagctttga cgatctggac acgctcgcga cactcgggtt
     1381 ccgaggcgag gcgttggcaa gcatttccgc cgtttcgtca ttcgccatgc gcagccgcac
     1441 agaggatcgt cccgccggca cttcagtcga ggtcgagttc ggggatcggc acgattcaaa
     1501 gactgttgcc gcgccggtcg gcacgacggt gtgcgtgcgc gatcttttcg gcaatgtccc
     1561 ggcgcggcgc aagtttctcc ggcaaccatc gactgagcat ggctatgtcg gacgagtggt
     1621 cgccgcttat gcctgcgcct acccgcatgt gcaattttcg ctaacccttg atgggcggaa
     1681 tgtgatctca accaacggat caggcgacca cctggctgcg gcgagcggcg tttttggtgt
     1741 cgaggtcggt aaggcagcgc tgctcgtagc gccactggaa gattcagcag cggtgccggg
     1801 ggtgagggtt gacggctgga tcggcgcgcc gaccgtttca cggtcacacc ggcaggacat
     1861 gatctttttc gtcaacgggc gctgggtgca gcaccggtcg atgtcgttcg cgctcgaaga
     1921 ggcgtaccac tcgctgctga tggtggggcg acatccgatc gcctttttgc atgtgcatgt
     1981 cgatccggct tcgatcgacg tcaacgttca tccaacgaaa gcggagatca agttccagga
     2041 cgagcgttcg gcctgtcgcg ccgtgcagcg atctgcccat gccgcgttgg cgcagtctcc
     2101 gcatgacgac ctgccgggag cgtcgttctc gcccgtattc gcgccggtcc accagccggc
     2161 gttcgcgatg ccgatgccgg cagggcgcgt ccaggcaatt gaacctgaaa atcagatcgg
     2221 cgctccggcg ccgtcggctg cgcatcagtc gggcgtgccg atgcttcggg tgctcgggca
     2281 ggtgggaggg tcttacatcg tcgccgaggg tccggacggg atgtacctga tcgaccagca
     2341 cgcggcgcac gaacgggtga tgtacgagaa gatcgcctca cagatgcgta ctgccggggt
     2401 cgatcggcag cagtttctcg atcctttgct tgtcgagctg ccgcccgaag aacacgccgt
     2461 gttcgagaag tcgcgcgatg acctggccgc gatcggattc gagatcgagc catttggcga
     2521 ggcgatcgtc gcggttcgct cggttccggc gctggtgaag ggcgtcgatg taccggagcg
     2581 gttacacctg attctccagg aactggccga cggcggagca ggggagtcgt ggttcgattc
     2641 cgtggcgatc agcgctgcct gtcacacggc gatcagagcc gggcaaacgc tttcgctggc
     2701 cgaaatgcgt gagctcgtca atgatctcga acgcacgagt cagccacgcg cttgcggtca
     2761 tggccgcccg acgatgctgc acatgagcca gttcgacctc gaaaagcagt tctcccgccg
     2821 ctgacgtccc cgctagcggg aatagcggcg gcggagatca ttgaccttga cgcgtgc
//
LOCUS       DATAOZ010000459         4080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_709951, whole genome shotgun sequence.
ACCESSION   DATAOZ010000459 DATAOZ010000000
VERSION     DATAOZ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709951"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VHR64_11700"
     CDS             <1..578
                     /locus_tag="VHR64_11700"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3303538.1"
                     /translation="AYPLDVTSDDSWQAAALAANNLLGPVDILVNNAGIAGRSAPTWE
                     LSVEEWQQVVDIDLTGVFLGCRAVLPDMLDRGYGRIVNIASIAGKEGNPNAVPYSAAK
                     SGVIGLTKAIAKEVATKGVIINAITPAVIETEILKQVSQSHIDYMTSRIPMGRVGQPH
                     EVAALAAFLCSEEITFSTGAVFDLSGGRATY"
     gene            628..1404
                     /locus_tag="VHR64_11705"
     CDS             628..1404
                     /locus_tag="VHR64_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3303539.1"
                     /translation="MEERTPVLATRGISKWFGPVRALTNIDFEVYSGEVVALIGDNGA
                     GKSTLINVITGVLPHEEGEIIFEGKSVNLTPQEARRLGIETVYQDLAIAPHLDSVANI
                     FLGREYRQPGLLGKMGFLDSKRMRSDTLQQLSRLRVRIKNIDRRMTTLSGGQRQGVAV
                     ARSVRWASKVVIMDEPTAALGVAQSGMVLDLIKEVRNSGIPIIFISHNMPHVFEVADR
                     IEVLRLGEMVAHLDPKTNSIDDAVSAMTGSIRMPTGVTIK"
     gene            1401..2447
                     /locus_tag="VHR64_11710"
     CDS             1401..2447
                     /locus_tag="VHR64_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303540.1"
                     /translation="MTSSALPEQTAAAPAEEPVERSLPSWFYILIVLMIMIVFFSIWH
                     GTKFADRTNFQNIALDASQLMLLAIGSTFVIITAGIDLSVSAMLVLSAIVGGKVMVHF
                     SGSPEQVRRYEFPDQKTGIPLGLAAAILTGLCVGLINGAIITKLKLPPFITTLGTLGI
                     CLGLANILSKGSSVPYVPIDITREIGAREIGGVRLLVIISATVVILAAITLRFTRFGR
                     YTFAIGSNATAARRVGINVDRHLIKVYALSGLLAGLAGAFDLARFGTANASTHSTDNL
                     NAISAVVIGGTSLFGGMGSIIGTVIGAFIPATLRNGLVIGGINPFYQQVLVGGILLAA
                     VYFDQRRRKAEERM"
     gene            2557..3594
                     /locus_tag="VHR64_11715"
     CDS             2557..3594
                     /locus_tag="VHR64_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3303541.1"
                     /translation="MELALYRITRRGLIKVGAGTAGLAVVGSRLSQSSAQESYKITLI
                     QGVAGDEFYVSMGCGAQQAATETGSQLTIQGTEKWDATLQIPLLNSVVQSKPDAILIA
                     PNDRTALIQPIQDALNAGIAVFTVDTLIEADIAIANIASDNVLGGKMAAQELAKLINE
                     TGSVYLQNTVPGASTTDQRQQGFEEEIKNYPNIKYIGVDFNNDDPTTAASQTAAKLQA
                     TPDLAGIFGTNLFSAEGAATAVKEAGKTGQIKIVGFDAGPKQVQDLREGVVDLLIGQH
                     PYDIGYKGVMMAVDYLKTKQAPTEKTVATGYTVVTKDNLDDPEVKKYLYVADCSEIPA
                     GTPSPESSPMS"
BASE COUNT          808 a         1301 c         1254 g          717 t
ORIGIN      
        1 cggcctaccc gctcgacgtc acgtcagacg attcctggca agccgccgcg ctggccgcga
       61 acaacctgct cggtccggtc gacatcctcg tcaacaatgc cggaatcgcc ggccgctccg
      121 ccccgacctg ggagctctca gtcgaagaat ggcagcaggt cgtcgatatc gatctgaccg
      181 gtgtcttcct cggctgccgc gcggttctgc ccgacatgct cgatcggggc tatggccgga
      241 tcgtcaacat cgcctcgatc gctggcaagg aaggcaatcc gaacgcggtt ccctactcgg
      301 ctgcgaaatc gggcgtgatc ggcctgacca aggcgatcgc caaggaggtc gcgaccaagg
      361 gcgtgatcat caacgcgatc acgccggccg tgatcgagac agagatcctg aagcaggtct
      421 cgcagtcgca tatcgactac atgaccagcc ggataccgat gggccgggtc ggtcagccgc
      481 acgaagtcgc agcgctggcg gcgttcctct gctccgagga gatcaccttc tccaccggtg
      541 cggtattcga cctgtccggc gggcgagcaa cgtattagat aatcgatcat cggcacgcag
      601 aacctgaatt tgacaaggca gtgacgtatg gaagaacgga caccggttct ggcgacgaga
      661 ggcatctcga agtggttcgg gccggtgcgc gccctgacca acatcgactt cgaggtctac
      721 tcgggcgagg ttgtcgctct gatcggcgac aacggcgccg gcaaatcgac gttgatcaac
      781 gtgatcaccg gagtgttgcc gcatgaagag ggcgagatca tcttcgaagg gaagtcggtc
      841 aacctgacgc cgcaggaagc gcggcggttg ggcatcgaga cggtctacca ggacctggcg
      901 atcgcgccgc acctcgattc ggtcgcgaac atcttcctcg gccgcgagta tcggcaaccg
      961 ggtctgctcg gcaagatggg cttcctcgac tcgaagcgca tgcgctcgga tacgctccag
     1021 cagcttagcc ggctccgggt gcggatcaag aacatcgatc gccgcatgac gacgctctcc
     1081 ggcggtcagc ggcagggtgt ggccgtagcg cgctcggtcc gctgggcaag caaggtcgtg
     1141 atcatggatg agccgacggc ggcgctcggc gtcgcgcagt ccggcatggt gcttgatcta
     1201 atcaaggaag tccgcaactc gggcatcccg atcatcttca tcagccacaa catgccgcac
     1261 gtcttcgagg ttgcagaccg aatcgaagtg ctgcgcctcg gcgagatggt ggcgcacctc
     1321 gatccgaaga cgaactcgat cgatgacgcc gtctcggcaa tgaccggatc cattcgcatg
     1381 ccgacaggag tgacgatcaa gtgacatcat cagcgctgcc agagcagacc gccgccgctc
     1441 ctgccgaaga gccagttgag cgatccctgc cctcatggtt ctacatcctg atcgtgctga
     1501 tgatcatgat cgtgttcttc agcatttggc acggcaccaa gttcgcggac cggacgaact
     1561 tccagaacat cgcgctcgat gcctcacagc tgatgctgct cgccatcggc tcgaccttcg
     1621 taatcatcac ggccggcatc gatctctcag tcagcgcgat gctcgtactt tcggcgatcg
     1681 tcggcggaaa ggtcatggtg cacttctcgg gctcgccgga gcaagtccgc agatacgaat
     1741 tcccggacca gaagaccggc atcccgcttg gactggccgc agcgatcctg accggactct
     1801 gcgtcgggct gatcaacgga gctatcatca ccaagctgaa actaccgccc ttcatcacga
     1861 cactcggcac gctgggcatc tgtctcggac tggcgaacat cctctcaaag ggctcgtcag
     1921 tgccgtacgt gccgatcgac atcacgcggg agatcggcgc gcgcgaaatt ggcggcgtgc
     1981 ggctcctcgt gatcatctcg gccacggttg tgatccttgc cgcgatcacg ctccgcttca
     2041 cccgtttcgg ccggtacacg tttgcgatcg gatcgaatgc gactgccgcg cgccgggtcg
     2101 gtatcaatgt cgaccggcat ctcatcaagg tgtacgccct gagcgggctt ctggccggcc
     2161 ttgccggcgc gttcgacctg gcccggttcg gcaccgcgaa tgcctcgacc cactcaacgg
     2221 ataacctgaa tgcgatctcg gcggtggtga tcggcgggac cagtctcttc ggtggcatgg
     2281 gctcgatcat cggaactgtc atcggcgcat tcatcccggc cacactgcgc aacgggctcg
     2341 tcattggcgg catcaatccg ttctatcagc aagtgctggt cggcgggatt ctgcttgcgg
     2401 ccgtctactt cgatcagcgg cggcgcaagg ccgaggagcg aatgtagctt ccgttgcact
     2461 tgcccagata ttgttgatac gatgacggtc catgctgaca agcaggcacg ggcctggtgc
     2521 ggccagcgca ccatgattga cgaggagaag agagccgtgg aattggccct gtatcgaatc
     2581 acgcgtcgag gcttgatcaa ggtcggagcc ggaactgccg gacttgccgt cgttggtagc
     2641 cgcctcagcc agtcttctgc ccaggaaagc tacaagatca ccctgatcca gggtgtcgcg
     2701 ggcgatgagt tctacgtgtc gatgggctgc ggcgcgcagc aggcggccac cgaaaccggc
     2761 tcgcagctca ccatccaggg caccgagaag tgggacgcca cgctgcagat cccgctgctc
     2821 aactcggttg tccagtcgaa accggacgcg attctgatcg cgccgaacga ccgcaccgcc
     2881 ctgatccagc ccatccagga cgcgctcaac gcaggcatcg ccgtcttcac ggtcgacacg
     2941 ctgatcgagg ccgacattgc gattgccaac atcgcctcgg acaacgtcct gggcggaaag
     3001 atggccgcgc aggagctggc caagctgatc aacgagaccg gctcggtcta cctccagaac
     3061 acggtgccgg gcgcctcgac gaccgaccag cggcagcagg gtttcgagga agagatcaag
     3121 aactacccga acatcaagta catcggtgtc gacttcaata acgacgaccc gaccaccgct
     3181 gcctcgcaga ccgccgccaa gctccaggcc accccggatc tggccggcat cttcggcacg
     3241 aacctgttca gcgccgaggg cgctgcgact gcggtcaagg aagccggcaa gacgggtcag
     3301 atcaagatcg tcggcttcga tgctggaccg aagcaagtcc aggacctgcg cgagggcgtc
     3361 gtcgatctcc tgatcggcca gcacccgtat gacattggct acaagggcgt catgatggcg
     3421 gtcgactacc tgaagaccaa gcaggcgccg acagagaaga ccgtcgcgac cggctacacg
     3481 gtcgtcacca aggacaacct cgacgatccg gaggtcaaga agtacctcta cgtcgccgac
     3541 tgctccgaga tcccggccgg gacgccgtcg cccgagtcct caccgatgtc gtaagtgcgc
     3601 cacggatagc cgtaggcagc ggggcggttt cccctcgtgg gagccgcccc aattttcgta
     3661 gtgccgtata catcgcccga acgtcttgcc gtgcccgata ccttcccgat tacggttggg
     3721 acgaactggc ggggagagga cgttgtcgga gcgatcccca gcccgcacga tatgtcggca
     3781 agggcggcac gtctggaatc agatcgccgc accgagtgcc atgagctccg cgcctgcccg
     3841 ccgcgcgatc gtgaacagaa ggaaaaccgc ttcaggtgag cgacttgtca atagaacaac
     3901 caggtatgcc cgtgcaaacc gcagcaacgt tcgccagccc cgaggaggag cgaccgccgc
     3961 tctgcccgaa gtgcaacaag cggctcgtca ttctcgcgtc gcaatcggcg cgggatgaga
     4021 acggcgggta tgtgcggcag cagctctggg ggtgcccacg cggtcatgcc acggcagtcc
//
LOCUS       DATAOZ010000460         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8604923, whole genome shotgun sequence.
ACCESSION   DATAOZ010000460 DATAOZ010000000
VERSION     DATAOZ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8604923"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VHR64_11720"
     CDS             <1..239
                     /locus_tag="VHR64_11720"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX3303542.1"
                     /translation="TADVQMEHSLFRHFIMTGPDVPGVVTASKMPGAIVEALFVSNDD
                     DAAVLSTPEGRNAIVTAYENAIVEYFERYPPEAK"
     gene            complement(410..544)
                     /locus_tag="VHR64_11725"
     CDS             complement(410..544)
                     /locus_tag="VHR64_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303543.1"
                     /translation="MAATLDGEGVDGGNGVDGSRPAVVGVDGAAGAVSLRGACTVREI
                     "
     gene            762..>1782
                     /locus_tag="VHR64_11730"
     CDS             762..>1782
                     /locus_tag="VHR64_11730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX3303544.1"
                     /translation="MRAERTLQSYEELVAALSADPGAVALVPAEMIDFRVQALTVGED
                     DPFDKIAGGNDTIRIGVVGDIVPGRNVHLHMLQYGDFTRPFQRVAPLLRSFDVTVANL
                     EGNLSDTLPQPADPHSVSFVSSPAMLEGFKLAGIDAVTLANNHSVWNDEGWGVQGLLD
                     TIAALDSQGIPYFGAAPTLTAARAPWVVAAERIRIAFLGIDAVTANYEVEPGAATGVL
                     DVDVGATNDRAGTNPYLAAQVAEDIAAVAAVADVVIPYFHLGAEYVAIVPPWAVQAAH
                     AAIDAGATMVVTNHPHLNQGMEVYAGKPIVYSPGNFILDQMWAAEVRSGYALEIVLRG
                     TAIVGL"
BASE COUNT          314 a          557 c          588 g          323 t
ORIGIN      
        1 cgacggcaga tgtgcaaatg gagcactcgc tcttccggca cttcatcatg acaggaccgg
       61 acgtacccgg tgtcgtgacg gcgagcaaga tgcctggcgc gatcgtcgag gcgctgttcg
      121 tctcaaacga tgacgatgcc gcggtgttgt ccacgcccga agggcggaac gcgattgtca
      181 cagcctacga gaacgcgatc gtcgagtact tcgaacgcta ccctccggag gctaagtgat
      241 caggcagtgc gccgggactt catgtgactg agcgccggcg gtcgaactcg cgagcagcag
      301 gagccggaac gatgcggcgg actgggcccc cgctaagccg ccgcgaagtg atcgtaggag
      361 cggttcccgt cgtcgccgca gcgatcggcg cggccggggc gctcgcggtt cagatttccc
      421 ggaccgtgca ggcgccgcga agtgacacgg ctccggcggc gccgtcgacg ccgacaactg
      481 ctggccggct accgtcgact ccattgcctc catcgacacc ctcaccgtcc agcgttgccg
      541 ccacgccgca gacaccgcgt gaactggcgc gggcactggg aaacgtgggt gtcccggaag
      601 gaatgatcct ggcggcatct cccagactcc ccctagccgg cgtcggtccg gaggatccct
      661 ggcggttact caccggacag gctgctaatt ggtctgaggt cggggcagca gtgccattgc
      721 ccgtgcaccc tttggccctc agtggggaat tagcaggcgg cgtgcgagcc gaacgcaccc
      781 tgcaaagcta tgaagagttg gtggcggcgt tgtcggccga ccccggcgcg gtggctctag
      841 ttccggcgga aatgatcgac tttcgcgtgc aggcgcttac cgtaggggaa gatgacccct
      901 ttgacaagat cgccggaggg aacgacacga ttcgaatcgg cgtcgtcggc gatatcgtgc
      961 cgggtcgcaa cgtccacctg cacatgctgc agtacggcga tttcacacgg ccgtttcagc
     1021 gggttgcccc gctcctgcgg tcgttcgacg tcacagttgc caacttagaa ggaaatctct
     1081 cggatacgct cccgcaacct gcggaccccc actccgtctc cttcgtctcc agcccggcga
     1141 tgctggaagg tttcaagctg gcagggatcg atgcggtcac cctcgcgaac aatcactcag
     1201 tgtggaatga cgaaggctgg ggggtgcagg gtctgctcga caccattgcg gcgctggata
     1261 gccagggcat cccgtacttc ggcgccgctc ccactctgac tgccgcccgg gcgccgtggg
     1321 tcgtcgccgc tgaaaggatc cggatcgcct tcctcggtat tgacgcggta accgcgaatt
     1381 acgaagtcga gcccggtgcg gcgaccggtg tgctcgacgt cgacgtgggt gcgacaaatg
     1441 accgagcggg gaccaatcct tacctcgccg cccaagttgc cgaggacatc gccgccgtag
     1501 ctgcagtcgc agatgtggtg atcccctact ttcatcttgg cgcggagtac gtcgcgatag
     1561 tgccaccttg ggccgtgcaa gcggctcatg cggcgattga cgctggcgca acgatggtgg
     1621 tgaccaacca cccgcattta aaccagggca tggaggtcta tgccggtaag ccgatcgtct
     1681 actcgccggg gaatttcatc ctcgatcaga tgtgggcggc ggaggtgcgg tcgggctacg
     1741 cgcttgagat cgtattgcgg ggcacggcga tcgtcgggtt gc
//
LOCUS       DATAOZ010000461        13181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4130768, whole genome shotgun sequence.
ACCESSION   DATAOZ010000461 DATAOZ010000000
VERSION     DATAOZ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13181
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4130768"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VHR64_11735"
     CDS             <1..316
                     /locus_tag="VHR64_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303545.1"
                     /translation="MDVIWSLRRSLTVPLAHVVDAKVEPVAGRPGWKVIGTGVPRGFS
                     AGKFRSKGKNEFWVADQKNAALVVTLRDEKYARFVLQVEDPQAAADMINAAAGGGARA
                     AA"
     gene            320..688
                     /locus_tag="VHR64_11740"
     CDS             320..688
                     /locus_tag="VHR64_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303546.1"
                     /translation="MAVSVTSAGSDLRVTISGWDAVWALKRSLTIPLAHVVGSSVEPV
                     SDKPSWRVFGTGIPGGLMAGWFRAKGQNEFWLVHRRTAALEIRLRDEKYARLMLQVEH
                     PEATAEMINALAGSDPRVAA"
     gene            complement(694..2790)
                     /locus_tag="VHR64_11745"
     CDS             complement(694..2790)
                     /locus_tag="VHR64_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX3303547.1"
                     /translation="MNIADPAPKPNKTVAALDNERRRLAAIVESSNDAIISENLDGTI
                     VSWNSAAERIYGYPTEEVIGKHITEVIPTARDGEMLDLMLRVRRGEQVPSFEVRRASK
                     DGTVRDVNVKVSPVRDESGEIIGVSAIVRDISDRVNIERELHNRVRQQAAVAQLGQHA
                     IAQTNLTDLLNHATELVRRTLDIEFATVLELIPGSQELLLRAGVGWKPGVIGMATVSA
                     LPISQSGYTLQSHEPVVISDLRAESRFTQVDLMREHGIVSGLSVVIQTPERPYGVLSG
                     HTTHQRTFSIDDVHFLEAIANILATAIARQQAYDLLGKRVEERTSELRMLLDITHDAA
                     ATLELQPLVGLILDRVKEVVDYTGAALFVLDDSGEGLNLLRYQGPIPQHTLAYRWSLA
                     THPHAREVVTRARPVIINDVYSDHPLAEAFRRASTSDLGEMRADFGSWMGVPMMLRDR
                     VIGMLSVEIEQINYYTGRHGELLLAVADHAAMAVENARLYERARGLAALEERQKLARE
                     LHDSVSQALFGIGLGARTARALLDRDPSKVADPLDYVVSLAEAALTEMRALIFELRPD
                     ALQQDGLVGILDKQAAALAARHKLDIKTDLGTEPEATFEVKEALYRIGQEALHNAVKH
                     AHASTIWVSLNQAGQSLTLEIVDNGVGFDPEGAFPGHLGLQSMRERAARMGAVIEVES
                     APGQGSRLSVVVPPAK"
     gene            complement(2976..3641)
                     /locus_tag="VHR64_11750"
     CDS             complement(2976..3641)
                     /locus_tag="VHR64_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3303548.1"
                     /translation="MTIRIVITDDHSVVRQGLRMFLTLDSEMDVVGEAENGVEAIKMV
                     EQLAPDVVLMDMLMPVMGGIEATGKIKQRFPDVEIVAMTSVLEDATVVGAVRAGAIGY
                     LLKNTDADSLCNAIRAAAAGQVQLAPEAAKLLMREVRLPEPKSPMGVEALTGRETDVL
                     RLVALGHANKEIAVELGIGEKTVKTHVSNVLSKLGVQSRTQAALYAARAGIIAPDAVG
                     ASG"
     gene            3784..4173
                     /locus_tag="VHR64_11755"
     CDS             3784..4173
                     /locus_tag="VHR64_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3303549.1"
                     /translation="MIKILLVDDQAAARMGLKLRLGLETDLEVLGEAVDGEDAVVQAT
                     RLAPDVVVMDVAMPIMDGIDATRVICASCDCRVVMLSLYDDQETRKRASDAGAVAFVT
                     KQEPDVALLEAIRKSREVEKSRSREEL"
     gene            4470..8309
                     /locus_tag="VHR64_11760"
     CDS             4470..8309
                     /locus_tag="VHR64_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303550.1"
                     /translation="MPAGRRHRFLLLIHALTVIALLLGSSALALPQVAAQDSTPAAAE
                     APTQPPPGESADPTQTPTPEPTIAIPEATATQSAPVDTATPEPSAAAPTATSTPAAAP
                     TATASPTREPSAIGPAATSGTLTIKAVDAAGNPVTFTCFDVYQDAGGGTLGSRVKTDY
                     CDNLSSGVVKVPLTAGSYVLVEIRRAPEFGTALNKPFTFSGDRSITVTHQLLTKIVIT
                     STDSVTGQPVAGACYTLEPQTPIILPPQYLSCDGFDDLNDGVTTINGVDRGTFTLIEF
                     SPPLGYFQVANRDITISTLSSPRSVTIAHEKGATLRISKRTESDQPLAGACFTLLANN
                     NGVPGSQIGENLCDATYGANDGTIEGFGFPTGDYFLHEQTTPVGYLPIADQPISLTAG
                     QTTELTVVDPLGGRVDVHAVDDRGNPIPDICPTVFRDAGGGVRGEQLQLPACDLGTNG
                     NDGVTTLIGLPPGNFLLVQPNPAWRILTGEYAPGPDVAFAIAAGQTRSVQIVSPVGGK
                     LVVTVVDQAHPETKLLGACFTVYTDAGSGTRGTAIGGLCDGLAPYDVKDGVVPLSGLA
                     AGSYVLAQTQAPAPFNLAADVTVTVALRDVKNITVALKPYGKVRIFKQTPEGAALPGA
                     CFIAIYGPGSAIPGNWASGTICDNADGANDGTIQISLADGKFELQEQTAPAGYVRAPN
                     TDIEITDGGVTDVTIVDPVPSTLILSFVNVWHEPVTGLCVDSTGRNETFGGLPGGGGC
                     DPDDGAKDGRITLTGLTWGAWSSRVGNPGSYTVPEISGQLAPGESKTVEVVLTEAQPK
                     ITVGPRIDGLSQTSVTISWETDQKMISAVTIETSGAATITLPETAGAPTRTHRITISG
                     LTANTLYTVRAVPTSPNGNVTSSALSVRTPSAELNSQVIASVVKSDASPLPGACLSVY
                     RDAGGGQVGTFVRSSCDWADADGNNGTITISGLSAGSFVLIQFYAPRYYKVAKPVLFT
                     LTSGQSRQLTVTNTRGGVTLKVFAREYNGPTLLNTCYSIYRNPGDGTIGAYVTDLCDN
                     FDAVDGFTTFSGIGPGDYYLWQYKTPSGYVADPIKKFTVLSGQTNRYITYSSRKTTDT
                     DNVVLKTIDSTGALKPGACFWLYRADNGGEPTDYVGDACDADDGRNDGVTYFTRVQAN
                     YSYAALEYVAPSGYVAGKMTYFTKAIDTLYSKRITQVAGGVPVKVTTLKGSTSTKLPG
                     ACYGVYRLIRTTYVAVSVACDSWDGSVDGVTIIRGVPAGTYRLYQNGTPAGYRAPTYI
                     TVTVGTTTKSVTVRTYP"
     gene            complement(8564..9016)
                     /gene="mscL"
                     /locus_tag="VHR64_11765"
     CDS             complement(8564..9016)
                     /gene="mscL"
                     /locus_tag="VHR64_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX3303551.1"
                     /translation="MIKEFRDFLMRGNIVDLAVAVVIGGAFGAVVTSLVNDIIMPIIG
                     IFGGNPDFTSNTFTINGSVFHWGSFLTALITFIIIAAVIFFVVVKPMNALMARIKRGE
                     VPAPDTVVPPTWEYRSASEAELNTLGAEGWELVGIGPTAFHLKRPATA"
     gene            9165..9782
                     /locus_tag="VHR64_11770"
     CDS             9165..9782
                     /locus_tag="VHR64_11770"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3303552.1"
                     /translation="MDLLGDRSWRALGRRSVALWCGVHARSVHAEEGIAMSESLIELF
                     RHNAWANQRLFDACEGLTDAQMDATVGGTFGSIRDTLAHIAGAQERIAAALAETEQVK
                     VRREREPFPGVAALRERIRASSDALVERAARAGDGDTVTTTWRDEDYTLPVWLLLVQA
                     INHATEHRAQVAAILTQLGIEPPNMDGWTYHEDQFGGDWSQLWSW"
     gene            complement(9937..11565)
                     /locus_tag="VHR64_11775"
     CDS             complement(9937..11565)
                     /locus_tag="VHR64_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX3303553.1"
                     /translation="MNKPATATPAAVHPPDESVMTHRQIMVVLIGVMAGMLLAALDQS
                     IVGTALPRIVSELGGLNHLSWVITAYLLTSTAVTPLWGKISDLYGRRLIFQLTIIIFL
                     IGSALCGLSQNMFELIAFRAIQGVGGGGLMAIALSIIGDVIPPRERGRYQGYFGAVFG
                     IASVAGPLLGGFFTDGPGWRWIFYINIPVGAVALVITTIALRMPVVRREHKIDYLGAG
                     MIVASVTSLLLYLNWSGETHGWTAPISIMLVALSVIFAVAFVWVESRAAEPIIPLRLF
                     RNTIFRIGNAFGFLSGVAMFGAIIFLPLFLQGVLGMSPTKSGLAMLPVVFGLFTSSIG
                     SGRLISRTGKYKIFPILGSSILIVGLVLLAQIGVGTPYWHIAVFGAIFGLGLGMTIST
                     IVVAIQNSVEMRDMGAATSSTTFFRSLGGAIGAAVFGAVLGIRLDHYIEQAFGAQAAG
                     AAAVDANNIQAMQALQEPTKHLVLTAFTNALNDVFLVCIPFIIIALIISLFLKEIPLR
                     SGAQQATPAPAPGNLAPATEDPEVTEAPFGAIGH"
     gene            complement(11694..12152)
                     /locus_tag="VHR64_11780"
     CDS             complement(11694..12152)
                     /locus_tag="VHR64_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3303554.1"
                     /translation="MSTTRQDQINAIEALFQQLTWRGRMRFSHRVEKYGLTMPQYLAL
                     TKILKLGPDATMSEVSDALHLPRSSMTSIADRLVGLGLVVRGSLESDRRAVSATITPA
                     GKKLIRTVEAKRNAELTAMLAELNTDELGEFARLLGTLLDALQQLPPDDG"
     gene            complement(12641..>13181)
                     /locus_tag="VHR64_11785"
     CDS             complement(12641..>13181)
                     /locus_tag="VHR64_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX3303555.1"
                     /translation="DILSGDTGNDTVEGGAGKDQISGGPGEDQLFGGFAIGTPDEQAQ
                     QGDAGDTISGGDDNDVIAGNTGNDRITGGLGDDRIAGNEGNDQFFGGDGDDELTDASG
                     DSRMFGAAGNDTLLSGPGNDQLDGGDGNDTLTAGAGDDTLYGRAGNDALDGGTDTDTC
                     NGGDGTDTATACETIIGIP"
BASE COUNT         2304 a         4139 c         4495 g         2243 t
ORIGIN      
        1 catggacgtc atctggtcgt tgcggcggag cctgacggtt ccgctggcgc acgttgtcga
       61 tgccaaggtc gagccggttg cgggtcggcc gggatggaag gtgatcggca ccggagttcc
      121 gcgtgggttc agcgccggca agttccgctc gaagggcaag aacgagtttt gggtcgcgga
      181 ccagaagaac gccgcgctgg tggttacgct ccgggacgag aagtacgcgc ggttcgtgct
      241 gcaggtcgag gatccgcagg ccgcggccga catgatcaat gcggcggccg gtggtggggc
      301 gcgggcggca gcgtaggcga tggccgtttc ggtaacgagc gccgggtcgg atttgagggt
      361 cacgatcagc ggatgggatg cggtctgggc gctcaagcgg agcctgacga tcccactcgc
      421 gcacgtcgtc ggttccagtg tcgagccagt gtcggacaaa ccaagctgga gggtcttcgg
      481 aaccgggatt ccgggtggtc tcatggcagg ctggttccga gcaaagggcc agaatgagtt
      541 ctggctggtg cacaggcgga cagcggcact tgagatccga ttgcgcgacg agaagtacgc
      601 ccgtctgatg ctgcaagtcg agcacccaga ggcgacggcc gagatgatca acgcgctcgc
      661 gggcagtgat ccccgcgtgg cggcctgacg cggctacttc gcgggtggga cgacgacgct
      721 gagacgggag ccctggcccg gggcgctttc gacctcgatc acggcgccca tccgggcggc
      781 acgctcccgc atcgattgca ggccgaggtg accggggaag gcgccctccg gatcgaaccc
      841 gactccgttg tcgacgatct ccagcgtcag gctctgtccc gcctggttga ggctgaccca
      901 gatcgtgctg gcgtgggcgt gcttgaccgc attgtgcagc gcctcctggc cgatccggta
      961 gagcgcctcc ttgacctcaa acgtggcctc cggctcggtt cccaggtcgg tcttgatgtc
     1021 gagcttgtgg cgcgcggcca gagcggcggc ctgcttgtcg aggatgccga ccaggccgtc
     1081 ctgctggagg gcgtccggac ggagctcgaa gatcaacgcg cgcatctcgg tcagcgcggc
     1141 ctcggccaat gacacgacat aatcgagcgg atcggcgacc ttggacgggt cgcgatcgag
     1201 cagggcgcgg gcggtgcggg cgccgagacc gatcccgaag agcgcttgcg agacggaatc
     1261 atgcagctcg cgggccagct tctggcgctc ttcgagggcg gccagacccc gcgcgcgctc
     1321 gtagaggcgg gcattctcga cggccatggc ggcgtggtcg gccacggcca aaagcagctc
     1381 gccatggcgg ccggtgtagt agttgatctg ctcgatctcg acggacagca tgccaatgac
     1441 ccggtcgcgc agcatcatcg gcacgcccat ccaggagccg aagtcggcgc gcatctcgcc
     1501 caggtcggac gtggaggcgc ggcggaaggc ctcagccagc gggtgatcgc tgtagacgtc
     1561 gttgatgatg accggccggg cgcgggtcac gacctcgcgc gcgtgcgggt gggtggcgag
     1621 ggaccagcgg taggcgaggg tgtgctgcgg gatcgggccc tggtagcgaa gcaggttcag
     1681 cccctcgccg gagtcgtcga gcacgaagag ggcagcgccg gtgtaatcga cgacctcctt
     1741 gacccgatcg agaatcaggc cgaccagcgg ctggagctcg agcgtggcgg cggcgtcgtg
     1801 ggtgatgtcg agcagcatgc gcagctcgct ggtgcgctcc tcgacacgct tgccgagcag
     1861 gtcgtaggcc tgctggcggg cgatcgcggt ggcgaggatg ttggcgatgg cctcgaggaa
     1921 gtggacatcg tcgatcgaga aggtgcgctg gtgcgtggtg tggccgctga gcacgccgta
     1981 cggccgctca ggcgtctgga tgacgacgct caggccgctg acgatcccgt gctcgcgcat
     2041 caggtcgact tgggtgaagc gcgactccgc ccggagatcg ctgatgacga ccggctcgtg
     2101 cgattgcagg gtgtagccgc tctgagagat cggcagggca ctaaccgtgg ccatgccgat
     2161 cacgcccggt ttccagccga cgccggcgcg gagcagcagc tcctggctgc cggggatgag
     2221 ctcgagcacg gtggcgaact cgatatcgag cgtgcggcgg accagctcgg tggcgtggtt
     2281 gagcagatcg gtcaggttgg tctgggcgat tgcatgttgg ccgagctggg ccacggcggc
     2341 ctgctggcga acgcggttgt gcagctcgcg ctcgatattg acgcggtcgc tgatgtcgcg
     2401 gacgatcgca gagacgccga tgatctcgcc cgattcgtcc cggactggcg agactttgac
     2461 gttgacatcg cggacggtgc cgtccttcga ggcccgccgg acctcgaagg agggaacctg
     2521 ctcgccgcgg cggacgcgga gcatcaaatc cagcatctcg ccgtcgcggg cggtcgggat
     2581 gacctcggta atgtgcttgc cgatgacctc ctcggtcggg tagccgtaaa tgcgctcggc
     2641 ggcagagttc cagctgacga tggtgccgtc gaggttttcg ctgatgatgg cgtcgttcga
     2701 ggactcgacg atggcggcga ggcggcggcg ctcgttgtcg agcgcggcga cggtcttgtt
     2761 gggcttcggt gcggggtcgg cgatattcat ggtagtgcgg tgatgttcat ttcattgacg
     2821 taacgcggaa ccgctgtgcc ccgcattcgt cgccggaagg tccgcgccag acagaaaaca
     2881 gaacctccgg cgagaccgtc gtggcctgcg gccacagcgg ggcacagcag ccccgcgcta
     2941 cgtttggtcc tatcgtatat ccgtggcacg cccgtctatc cggaggcgcc gacggcatcc
     3001 ggcgcgatga tcccggcgcg ggcggcatag agtgcggcct gcgtgcggct ctggactccg
     3061 agcttcgaga ggacgttgct gacgtgggtc ttgaccgtct tctcgccgat gccgagctcg
     3121 accgcgatct ccttgttggc gtgaccgagc gcgaccaggc ggaggacatc ggtctcccgg
     3181 ccggtgaggg cctcgacgcc catcggcgat ttcggctcgg gcaggcgcac ctcccgcatc
     3241 agcagcttgg ccgcctcggg cgcgagctgg acctgaccgg cggcggcggc gcggatggcg
     3301 ttgcagagcg aatcggcgtc ggtgttcttc aataaatagc cgatcgcgcc ggcccggacc
     3361 gcgccgacga ccgtggcgtc ctcgagcacg ctggtcatgg cgacgatctc gacatcgggg
     3421 aagcgctgct tgatcttgcc ggtcgcctcg atcccgccca tcaccggcat gagcatgtcc
     3481 atgaggacga catcgggcgc gagctgctcg accattttga tcgcttcgac gccgttctcg
     3541 gcctcgccga cgacgtccat ctcggagtcg agcgtaagaa acatccgtag tccctggcgc
     3601 actacggaat gatcgtcggt gataactatt cgtatggtca ttgatcccct ccgccgaaag
     3661 tcggagatcg cgtttcgact aatgccgcag gtacacgcat ttgctttgca cgtacaaatg
     3721 tagcagggcc gattacttat cgatacgaac cagcggcccg gtacgtgagg aattaacgca
     3781 cggatgatca agattttgtt agttgacgac caggccgcgg cccgaatggg gctcaagctg
     3841 cggctgggcc tcgagacgga cctggaggtg ctgggcgagg cggtcgacgg ggaggacgcg
     3901 gtcgtacagg cgacgcggct ggccccggat gtggtggtaa tggatgtggc gatgccgatt
     3961 atggacggta tcgatgcgac gcgggtgatc tgcgcatcgt gcgattgccg ggtcgtgatg
     4021 ttgagcctct acgatgatca ggagacgcga aagcgggcga gcgacgcggg cgcggtggcg
     4081 ttcgtgacga agcaggagcc ggatgtggcg ttgctggagg cgatacggaa gagtcgagaa
     4141 gtcgagaagt cgaggagtcg agaagagttg taggggcgca ttgcaatgcg cccgtttgcg
     4201 aagacgaata caggatgaaa cgagcgccgg gcgacttgcc cggcgctttt gtgtgcctgc
     4261 ggtgggtgtt tcccggcctt cccgataatt ccaaacatac gcatacctat tgcaaaccaa
     4321 ctgttctgta cgtaaagttc gacaaacgca atcggaacaa cgacctggcg atgagcgccg
     4381 atggatcaga tgtcccactg atcgacgccc cggcgtcgcc ctgccgtcga ccgcgctttc
     4441 gttcttcgct tctattcgag gatcgctcta tgcctgccgg ccgacgccat cgcttcctgc
     4501 tcctcatcca cgcgctcacg gtcattgccc ttcttctcgg tagctccgct ctcgctcttc
     4561 cccaggtcgc cgcccaggac agcactcccg cggccgccga agcacccacc cagccgccgc
     4621 ccggcgagtc cgccgatccg acccagacgc caacgcccga gcccaccatc gcgatccccg
     4681 aagcgaccgc aacccagtcc gcgccggtag acaccgcaac gccggaaccg tctgcggccg
     4741 ctccaacagc cacatcgacc ccggccgcgg cgccgacggc aaccgcaagc ccaacacgcg
     4801 aaccgtcggc gatcggaccc gccgctacgt ccggcaccct gaccatcaag gccgtcgatg
     4861 ccgccggcaa tcccgtcacg ttcacctgtt tcgatgtcta ccaggatgcc ggaggcggca
     4921 cgctcggcag ccgcgtcaag accgactact gtgacaacct cagtagcggc gtggtcaagg
     4981 ttccgctgac cgccggctcc tacgtgctcg tcgagatccg ccgggcaccc gagttcggca
     5041 cggccctgaa caagccgttc accttcagcg gcgatcgctc gatcaccgtc acgcaccaac
     5101 tgctgacgaa gatcgtcatc accagcaccg atagcgtcac cggccaacct gtcgccggcg
     5161 cctgctacac gctcgagccg cagaccccga tcatcctgcc gccccaatat ttgagctgcg
     5221 acggcttcga tgacctgaac gacggcgtca cgaccatcaa cggcgtcgac cgcggcacgt
     5281 tcaccctcat cgagttcagc ccgccgctcg gctatttcca ggtggccaac cgcgacatca
     5341 ccatctcgac cctctcctcg ccgcgctccg tcacgatcgc gcacgagaag ggcgccacgc
     5401 tccggatctc caagcgaacc gagtcggatc agcccctcgc cggcgcctgc ttcaccctcc
     5461 tggcaaacaa caacggcgtc cccggaagcc agattggcga gaatctctgc gatgccacct
     5521 acggcgccaa cgatgggacg atcgaaggat tcggctttcc gaccggcgat tacttcctgc
     5581 atgagcaaac gacgcccgtt ggctacctgc caatcgccga tcagccgatc tccctcaccg
     5641 ccggtcagac caccgagctg accgttgtcg atccgcttgg cggccgcgtc gatgtccatg
     5701 ccgtagacga ccgcggcaac ccgatccccg atatctgccc cactgtcttc cgggatgcgg
     5761 gcggtggagt gcgcggcgag caattgcaac tcccggcgtg tgatctcgga acgaacggga
     5821 acgacggcgt gacgacgctg atcggtctgc cgcccggcaa cttcctcctc gtgcaaccga
     5881 atccggcgtg gcgaatcctc accggcgagt acgcccctgg acccgacgtg gccttcgcga
     5941 tcgcggccgg tcagaccagg tcggtccaga tcgtcagccc ggtcggcggc aagctcgtcg
     6001 tcactgtcgt cgatcaggcc cacccggaaa caaagctgct cggcgcctgc ttcacggtct
     6061 ataccgatgc cgggagcgga acccgcggaa ccgcgatcgg cggactctgc gatggccttg
     6121 ccccatacga cgttaaggat ggcgtcgttc ccctcagtgg tcttgccgcc ggttcgtatg
     6181 tcctcgccca gacccaggcg cctgcgccat tcaatctcgc cgcggatgtc accgttacgg
     6241 tcgccctccg cgatgtcaag aacatcactg ttgcgctcaa gccctacggc aaggtccgca
     6301 tcttcaagca gacacccgag ggcgccgcgc tgcccggagc ctgcttcatt gccatctacg
     6361 gacccggcag cgcaattccg ggcaactggg catccgggac gatctgcgac aacgcggacg
     6421 gcgcgaatga cggcacgatc cagatctctc tcgccgacgg aaaattcgag ctccaggagc
     6481 aaaccgcgcc cgccgggtat gtgcgcgcgc caaacaccga catcgagatc acggacggcg
     6541 gcgtcaccga tgtcacgatc gtcgaccctg ttcccagcac gctgatcctc agcttcgtca
     6601 acgtctggca cgagccggtc accggcctct gtgtcgattc gaccggccgg aacgagacct
     6661 tcgggggcct gccgggcgga ggcggttgcg atcccgacga tggcgccaaa gacggccgca
     6721 tcacgcttac tggtcttacc tggggggcct ggagctcacg cgtcggcaat cccggctcgt
     6781 acacggtacc cgagatctcc ggccagctcg ctccgggcga gagcaagacc gtcgaggtcg
     6841 tcctcaccga agcgcagccg aagatcacgg ttgggccgcg cattgatgga ctttcccaga
     6901 cgtcagtcac catctcctgg gaaaccgacc agaagatgat cagcgccgtg accatcgaaa
     6961 catccggcgc cgcaacgatc acgctcccgg aaaccgccgg cgcgccgacc cgcacgcacc
     7021 ggatcaccat ctccggcttg accgccaaca cgctttacac cgtccgcgcc gtgccgacga
     7081 gcccaaatgg caacgtcacg agctcggcgc tgtccgtccg cacgccgtcg gctgagctca
     7141 acagccaggt gatcgcgagc gtcgtcaagt ccgacgcctc accactgccg ggcgcctgcc
     7201 ttagcgtcta ccgggatgcc ggcggtggcc aggtcggcac gttcgtgcgc agctcgtgcg
     7261 actgggcgga cgcggacggc aacaatggca ccatcacgat tagcggcctc tcagccggct
     7321 ccttcgtgct gatccagttc tacgcgcctc gctattacaa ggtcgccaaa ccggtgctgt
     7381 tcacgctgac cagcggccag tccaggcagt tgaccgtgac caatacgcgc ggcggcgtga
     7441 ccctgaaggt cttcgctcgc gaatacaatg gaccgacctt gctgaacacc tgctactcca
     7501 tctaccgcaa cccgggcgac ggcacgatcg gcgcgtatgt gaccgacctg tgcgacaact
     7561 tcgacgcggt ggatggcttc accaccttca gcggcatcgg ccccggcgat tactacctct
     7621 ggcaatacaa gacgccatcc gggtacgtcg ccgatccgat caagaagttc accgtgctct
     7681 ccggccaaac taaccggtac atcacctact ccagcaggaa aacgaccgac accgataacg
     7741 tggtgctgaa gacgatcgac agcaccggcg cgctgaaacc gggcgcctgc ttctggctct
     7801 atcgggccga caacgggggc gagcccaccg actatgtcgg cgatgcctgc gatgcggacg
     7861 acggccgcaa cgacggcgtc acctacttca cgcgggtgca ggcgaattac tcgtacgccg
     7921 cgctcgagta cgtcgcccca agcggctacg tggccgggaa gatgacctac ttcaccaagg
     7981 cgatagatac gctctacagc aagcgcatca ctcaggtcgc cggcggggtc ccggtgaagg
     8041 tcaccacgct caagggcagt accagcacga agctgcccgg cgcgtgctac ggcgtttatc
     8101 gcctgatccg gacgacctac gtcgcggtga gtgtggcgtg cgattcgtgg gacggcagcg
     8161 tggacggtgt cacgatcatc cggggcgtcc cggcgggcac ctaccgcctc tatcagaacg
     8221 gcaccccggc cggctatcgg gcgcctacct acatcaccgt cactgtcggg acgacgacca
     8281 aatcagtcac cgtgcgcacc tacccgtaat agcggccggg tgcgcggtgc ccggggatag
     8341 cgtttccctg ggtgagttgg ttttcggggt tcggttttcg gttttccgtt ttccgatgac
     8401 ggagtgggtt gggaacgcag agcgagcgta tcgtgaggag gaatacagga tgaaacgagc
     8461 gccgggcgac ttgcccggcg cttttgtgtg ggtggcgata cgggcgcttc ccgggcctga
     8521 agacccgggc tgatatatga acccggctca agccggccgg gtgtcaggct gtggccgggc
     8581 gcttgagatg gaaggcggtt gggccgattc cgacgagctc ccagccctcg gcgccgagtg
     8641 tattgagctc tgcctccgag gcggagcggt actcccaggt cggcggaacg acggtgtcgg
     8701 gggcagggac ctcgccgcgc ttgatccgcg ccatgagggc attcatcggc ttgacgacga
     8761 cgaagaagat gaccgcggcg atgatgatga acgtgatcag cgcggtcagg aaggaacccc
     8821 agtggaacac gctgccgttg atggtgaagg tgttcgaggt gaagtccgga ttgccgccga
     8881 agatgccgat gatcggcatg atgatgtcgt tgaccagcga cgtcaccacc gcgccgaacg
     8941 cgccaccgat gacgaccgcc acagccagat cgacgatgtt gccccgcatc aggaaatcgc
     9001 gaaattcctt gatcacccgg accctcccca tacgctcgcc cataaacgcc agaaaagcgt
     9061 agcagatcgg gcgttcgtgc cgcagcgtac gtcaggttta gagcgcgcgg tgtaggccgt
     9121 cacctgacag gagtacgcat gccgcaggga taatcgcgag cgtgatggac ctcctgggtg
     9181 atcgttcctg gcgcgcgctc ggcaggcgca gcgtcgcgct atggtgcggt gtgcacgctc
     9241 gttcggtgca cgcagaggag gggatcgcga tgtccgagag cctgatcgag ctgttccggc
     9301 acaatgcctg ggcgaaccag cgcctgttcg atgcatgcga ggggttgacc gacgcgcaga
     9361 tggacgcgac ggtcggcggc acgttcggca gcattcgcga caccctcgcg catatcgccg
     9421 gcgcacagga gcgtatcgcc gcggccttgg ctgagacgga gcaggtcaag gtgcgccgcg
     9481 agcgcgaacc gttccccggt gtcgcggcgc tgcgcgagag gatccgcgcg agcagtgacg
     9541 cgctggtgga gcgggccgct cgcgccgggg atggggacac cgtcacgacg acgtggcgcg
     9601 acgaggacta cacgctgccg gtctggctcc ttctcgtcca ggccatcaac cacgccaccg
     9661 agcatcgcgc gcaggtcgcc gcgattctca cccaactcgg catcgagccg cccaacatgg
     9721 acggctggac ctaccacgaa gaccagtttg gcggggactg gagccagcta tggtcgtggt
     9781 agccgccgcg tgggtcgggt gatgctctct cccaacgact aacaccccgt tgatatgcac
     9841 caaccccgtc accggtccgg gcacccgaac ggttcgttcc ggtaactccc gggcagatgc
     9901 aatctgcccc tacccgttcc tcaacccgca acccaatcag tggccgatgg cgccgaaggg
     9961 ggcttccgtt acctcggggt cttcggtggc gggggcgagg ttgcctgggg ccggagcggg
    10021 cgtcgcttgc tgggcgccgc tgcgcagcgg gatctccttc aagaacagcg agatgatgag
    10081 cgcgatgatg atgaagggga tgcagaccag gaagacgtcg ttcagcgcgt tggtgaaggc
    10141 agtcaggacc aggtgcttgg tcggctcctg gagcgcctgc atggcctgga tgttgttggc
    10201 atccaccgcc gcggcgccgg cggcctgcgc gccgaaggcc tgctcgatgt agtgatcgag
    10261 tcggatcccg agcacggcgc cgaagacggc cgcgccgatc gcgccgccca gcgaccggaa
    10321 gaacgtcgtc gagctggtgg ccgcgcccat gtcccgcatc tcgaccgagt tctggatcgc
    10381 gacgacgatg gtcgagatgg tcatgccgag gccgagaccg aagatcgcgc cgaaaacggc
    10441 gatgtgccag tagggcgtgc cgacgccgat ctgggccagc aggacgaggc cgacaatcag
    10501 gatcgatgag ccgaggatcg ggaagatctt gtacttgccg gtccgcgaga tgagacgacc
    10561 cgaaccgatc gaggaggtga agaggccgaa gacgaccggc agcatggcca ggccggattt
    10621 ggtcggcgac atgcccagaa cgccctgcag gaaaagcggc aggaagatga tcgcgccgaa
    10681 catggcgacg ccggagagga agccgaaggc gttgccgatc cggaagatgg tgttgcggaa
    10741 gaggcggagc gggatgatcg gctcggccgc gcgcgactcc acccagacga aggccacggc
    10801 gaaaatgacc gaaagggcca ccagcatgat cgagatcggc gcggtccagc cgtgggtctc
    10861 gccggaccag ttgaggtaga gcaggagcga ggtgaccgag gcgacgatca tgcccgcgcc
    10921 gaggtaatcg atcttgtgct cgcggcggac aaccggcatg cggagcgcga tggtcgtgat
    10981 gacgagggcg acggcgccga cggggatgtt gatgtagaag atccagcgcc agccggggcc
    11041 gtcggtgaag aagccgccga ggagcggtcc ggccacgctg gcgatgccga agaccgcacc
    11101 gaaatagccc tggtaccggc cgcgctcgcg aggcggaatg acgtcgccga tgatgctgag
    11161 ggcgatcgcc atcagaccgc ctccgccgac gccctggatg gcgcggaagg cgatcagctc
    11221 gaacatgttc tggctcagac cgcagagagc ggagccgatc aggaagatga tgatcgtgag
    11281 ctgaaagatc aggcggcgac cgtagaggtc ggagatcttg ccccagagcg gcgtgacggc
    11341 ggtggaggtc agcaagtagg cggtgataac ccacgagagg tgattgaggc cgccgagctc
    11401 gctgacgatg cggggcaggg cggtgccgac gatgctctgg tcgagcgcgg cgaggagcat
    11461 cccggccatg acgccgatca gcacgaccat gatctggcgg tgggtcatga ccgactcgtc
    11521 gggcggatgg acggcggctg gggtggcggt tgctggctta ttcacgcgtt caattcctgg
    11581 tttcaattcg tctctcggaa gcatcggccc gggtggggcg cacaatcggg tatgaggtgt
    11641 agttcgtgac agttctgccg ggcgtgcgag cgcgacatcg gctcgcccgg ctgttagccg
    11701 tcatcgggcg ggagctgctg caacgcgtcg agcagcgtgc cgagcagacg ggcgaactcg
    11761 ccgagttcgt cggtgttgag ctcggcgagc atggcggtga gctcggcgtt gcgcttggcc
    11821 tcgaccgtgc ggatgagctt cttgccggcc ggcgtgatgg tcgcgctgac ggcgcggcgg
    11881 tcggattcga ggctgccgcg aacgacgagg ccaaggccga cgaggcggtc cgcgatgctg
    11941 gtcatggagc tacggggcag atggagcgcg tcgctgacct cgctcattgt ggcatcgggg
    12001 ccgagtttca ggattttggt cagggcgagg tactggggca tggtcagccc gtacttctcg
    12061 acgcggtggc tgaagcgcat gcgtccgcgc caggtgagtt gttggaagag ggcctcgatc
    12121 gcgttgatct ggtcctgtcg cgttgtcgac atggtcgctc gcggattggc cgggacgtgg
    12181 tgcggcccgg gtggcggtac gatgggagat acgttgtcgg atagtacgac gtcgtacgag
    12241 atggggagta tgggagatgt ggggtggggt gtcaaggggg aggcgagcag ttaagaacgt
    12301 agtggcgagg ctccgtactc gtcttgagtt actgtctgat tcgtggtgct ggcgagtggt
    12361 gatccggcgg cgcccggcga taaatcaccg ggctacatcc aataagccag ctaaagcaag
    12421 ccaatggggg ccgatttccg gaaacgtagc gcggtgcggc agccccgcac agcacccata
    12481 cccacgcacc gtggacgggg ccgaaatgcg gatggcgttg gttttcccag tgacgcgatc
    12541 tcccggcgag actagcgggg catggtaacc ccgcgctacg ttttatgggc gcatttcatg
    12601 ttcggctatt ggccgagtgg tgacgcgcgg tcggtcctcc ctacgggatg ccgatgatgg
    12661 tttcgcaggc ggtggcggtg tctgtgccgt cgccgccgtt gcaggtgtcg gtgtcggttc
    12721 cgccgtcgag cgcgtcgttg ccggcccggc cgtaaagggt gtcatctccc gcgccggcgg
    12781 tgagggtgtc gttgccgtcg ccgccgtcta gctggtcgtt gccgggtccg gagaggagtg
    12841 tgtcgttccc ggccgcgccg aacatgcggc tgtcgccgga ggcatcagtc agctcgtcgt
    12901 cgccgtcccc accgaagaac tgatcgttgc cctcattgcc ggcgatgcga tcgtcgccca
    12961 gaccgccggt aatgcgatcg ttgccggtat tgccggcgat cacgtcgttg tcatcgccgc
    13021 ccgagatggt gtcgccggca tcaccctgct gcgcctgctc gtcgggcgtg ccgatcgcga
    13081 agccaccgaa gagctggtcc tcgccgggtc ctccgctgat ctggtccttg ccggcgccgc
    13141 cctcgacggt gtcgttaccg gtatccccgc tgaggatgtc g
//
LOCUS       DATAOZ010000462         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1500844, whole genome shotgun sequence.
ACCESSION   DATAOZ010000462 DATAOZ010000000
VERSION     DATAOZ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500844"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VHR64_11790"
     CDS             complement(<1..205)
                     /locus_tag="VHR64_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303556.1"
                     /translation="MSSQRYEVRPVGWVESPLIDRSAAPKQGDEGALVARIVFEPQFR
                     EAARDLLPGDEVLVLTWLHQGDRT"
     gene            complement(239..583)
                     /locus_tag="VHR64_11795"
     CDS             complement(239..583)
                     /locus_tag="VHR64_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3303557.1"
                     /translation="MTSGLKTVIYPVKDLASAKKLYGTLFGVEPYADQPYYVGFNIEG
                     QDVGLDPNGHSKGMTGPLPYWHVSDIRASIDDLVAAGGELQQEPSDVGGGNLIATVKD
                     TDGNVIGLFKAV"
     gene            669..1556
                     /locus_tag="VHR64_11800"
     CDS             669..1556
                     /locus_tag="VHR64_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3303558.1"
                     /translation="MHESDWLATQFEHHRNRLRAVAYRMLGSQSEADDAVQETWIRLS
                     RSNADEVENFGGWLTTVVSRVSLDMLRSRRSRREEPFDPATTEMLIPDEDGIDPEREA
                     VMADAIGPALLVVLDTLTPAERLAFVLHDLFGVPYDEIASIVARTPAAARQLASRARR
                     RVRGASMLAQNVDQIRLYTIVDAFLAAARDGDFDSLVSLLDPGVELRADQAAIEMGSA
                     PVLRGARDVAGRFAGGAQVARTALIDGVAGAVWMTGRQPRIIFSFTFSDDRIAAIEII
                     ADPVRLKTIDVATRYPSRN"
     gene            complement(1631..>2291)
                     /locus_tag="VHR64_11805"
     CDS             complement(1631..>2291)
                     /locus_tag="VHR64_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX3303559.1"
                     /translation="TNFISSIGEGKRVADLIDQQLRGTKPRVREMELTRVPTEFVATP
                     NPLMAEGIAEWSLTAMSRRLVWGDDYAGVGRQVMPSLPVGDRGIGTLDTTLEVEVGFT
                     KPIGFEEAKRCLQCQLNIFIDGNRCILCNGCVDACPHRCIEMISPERIYAIDNDVELA
                     EMARAELGPYAAAMVIDERSCIRCGICVDWCPTECLTMDHFRVTPPETRESVDLAIVA
                     D"
BASE COUNT          407 a          742 c          714 g          428 t
ORIGIN      
        1 tcgtgcgatc tccctggtgc aaccaggtca gcaccagcac ctcgtcaccc ggaaggagat
       61 cgcgggcggc ttcgcgaaac tgcggctcga acacgatgcg cgcgaccaga gcgccttcat
      121 cgccctgttt gggagcggcg gagcgatcaa tcagcggcga ttcgacccaa ccgaccggtc
      181 gaacctcgta cctttggctg ctcacgctcc gctctcccca caatcggctg acgcgtccct
      241 agaccgcctt gaacaacccg atcacattgc catccgtatc tttcactgtc gcgatcaggt
      301 taccccctcc gacatcgctg ggctcctgct gcagctcccc accggccgcc acaaggtcat
      361 cgatgctcgc ccggatgtcg gagacatgcc aataggggag cggcccggtc atgcccttgc
      421 tgtgcccgtt cgggtcgagg ccgacatcct gtccttcgat gttgaacccg acgtaatatg
      481 gctgatccgc atacggctcg actccgaaca gtgtgccgta gagtttcttt gcactcgcca
      541 aatccttgac ggggtagatc actgtcttga gaccggacgt catgacgcac tccttgtact
      601 ggttctgtca catcgaccga tgttcggccg tcaacactat gacggaccac gtaggagatg
      661 tgtgaccaat gcatgagtct gactggctgg cgacccaatt cgagcatcac cggaaccggc
      721 tgcgagcggt ggcgtaccgg atgctcggct cccagagtga ggcggacgat gccgttcagg
      781 agacgtggat tcggctgagc cgcagcaacg cggatgaggt cgagaatttt ggcggctggc
      841 tgacgacagt cgtctcccga gtctcactcg acatgctccg atcgcgcagg tctcgtcgcg
      901 aggagccgtt tgacccggcc acgacagaga tgcttatccc ggatgaggat ggaatcgacc
      961 ccgagcgcga ggcggtgatg gccgacgcga tcggcccggc actcctcgtc gtgctggata
     1021 cgctgactcc ggcggaacgg ctggcattcg tgctgcatga tctgtttggc gttccgtatg
     1081 acgagatcgc gtccatcgtc gcgcgaacac cggccgccgc caggcaactc gccagtcggg
     1141 cccgccggcg ggtgaggggc gcttcgatgc tggcccagaa cgtcgatcag atccggctgt
     1201 acaccatcgt cgacgccttc cttgcggccg cacgcgatgg tgatttcgat tcgctggtca
     1261 gcctcctcga tccgggcgtg gagttgcgcg ccgaccaggc ggcaatcgaa atgggttcgg
     1321 ccccggtgct tcgcggagcg agggacgtcg ctgggcgatt tgcgggaggt gcccaggtcg
     1381 cccgcactgc gctcatcgat ggcgtggcgg gagcggtctg gatgaccggg agacaacccc
     1441 ggatcatatt cagcttcacc ttctcggatg accgcatcgc ggccatcgaa atcatcgcag
     1501 acccggtgcg cctcaagacg atcgacgtcg ccacgcggta cccatcccgc aactgatcgg
     1561 gaagcagaaa cccccggacg ggaaaacgtc cgggggtttc tcttgtctat ctggtcaatg
     1621 cggtaccggc tcagtcggct acgatcgcga ggtcgacgct ttcgcgcgtt tccggcggcg
     1681 tgacgcggaa gtgatccatc gtcaggcact cggtcgggca ccagtcgacg cagataccgc
     1741 aacggatgca actccgctcg tcgatcacca tggccgcggc gtacggacca agttcggcgc
     1801 gcgccatctc ggccagctcc acgtcgttgt cgatcgcgta gatccgctcc ggcgagatca
     1861 tctcaatgca gcggtgcggg caggcatcga cgcagccgtt gcagagaatg cagcggttgc
     1921 cgtcgatgaa gatattgagc tggcactgga ggcagcgctt ggcctcctcg aagccgatcg
     1981 gcttggtgaa accgacctcg acctcgagcg tcgtatcgag cgtgccgatg ccgcgatcac
     2041 cgacgggtag cgagggcatg acctgccgcc ccacgcccgc gtagtcgtcg ccccagacga
     2101 ggcggcggct catcgcagtc aggctccact cggcgatgcc ctccgccatc agcggattcg
     2161 gcgttgcgac gaactcggtc gggacgcggg tcagctccat ctcacgaaca cgcggcttgg
     2221 tgccccgaag ctgctgatcg atcagatccg cgactcgttt gccctcgccg atcgacgaga
     2281 tgaagttggt c
//
LOCUS       DATAOZ010000463         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8079400, whole genome shotgun sequence.
ACCESSION   DATAOZ010000463 DATAOZ010000000
VERSION     DATAOZ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8079400"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VHR64_11810"
     CDS             <1..770
                     /locus_tag="VHR64_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX3303560.1"
                     /translation="GLNDGLSVPFLLFFIALATAGGEGGTASLMRFILDQLGLGVLVG
                     VATGLAGGWLLAHADRKEWMADSFRQVGLVALPVLCLLGSEMAGASMFISAFVAGLAV
                     QIGFRDVSAHSLEFAEEWGQVLNLSVFFLFGLLVALQWQQIDAAAVLYAVLSLTVVRM
                     LPVALALVGARLSMLTVIFMGWFGPRGLASIVLGLVYLQQEANLPGESTITLAVMATV
                     LISIFAHGLSAAPGIELYAGKVARLDAGAPEHQEVKA"
     gene            798..1271
                     /locus_tag="VHR64_11815"
     CDS             798..1271
                     /locus_tag="VHR64_11815"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX3303561.1"
                     /translation="MRRTIGGIDMLRQLLVGAAVSACNIAIHALVMTAVVRVAQSAGA
                     NDTSRRSLQLIAVMMATVSVLMAAHICEVVVWSLAYAMTDAAPAGTDLLYFAFVNYTT
                     LGYGDVTPVERWQLLGPMTAMNGVLLFGWSTAVIFEVLRRTVRLGAAATSNMKER"
BASE COUNT          227 a          447 c          520 g          315 t
ORIGIN      
        1 ccggtctcaa tgacggactt tcggttccgt tcctcttgtt cttcatcgcc ctggcgacag
       61 ccggcggcga aggtggcacc gcaagcttga tgcgattcat cctggaccag ctcggcctcg
      121 gcgtcctggt cggtgtcgcg actgggctcg ccggaggatg gttgctggcc cacgccgacc
      181 ggaaggagtg gatggcggac tcgttccgac aggtcggcct ggtcgcgctg ccggtgttat
      241 gcctgctggg gtccgaaatg gcgggcgcga gcatgttcat ctccgcgttc gtcgccggcc
      301 tggcggtgca gatcggcttc agggacgtga gcgcgcacag cctcgagttc gcggaagaat
      361 ggggacaagt gttgaatctg tccgtgttct tcctcttcgg ccttctggtg gcgctacaat
      421 ggcagcagat cgacgcggcg gcggtgctgt acgcggtgct cagcctgacc gtcgtgcgga
      481 tgttgcccgt cgcactcgcg ctcgtcggcg cgcggctgag catgctgacg gtcattttca
      541 tgggttggtt cggcccgcgc gggttggcgt ccattgttct cggactggtc tatctgcagc
      601 aggaagcgaa tctgccgggg gagtcgacga tcacacttgc cgtcatggcg accgtgctga
      661 tcagcatttt cgcccatgga ctgagcgccg cccccggcat cgagctttac gccggcaagg
      721 tcgcgaggct cgacgccggg gcgccggaac accaggaggt caaggcataa ggcggcgcat
      781 gtccggcctg gatcgatatg cgacggacga tcggaggcat cgacatgtta cgccaacttc
      841 tggtgggagc tgctgtcagc gcgtgcaaca ttgcgatcca tgcgttggtg atgaccgccg
      901 tggtgcgggt ggcacagagc gcaggcgcga atgacacatc acggcgatcg ctgcaattga
      961 tcgccgtcat gatggcgacc gtgtcggtcc tgatggccgc ccatatttgc gaggtggttg
     1021 tttggtcgct ggcctacgcg atgaccgatg ccgcacccgc cggtaccgat ctcctctatt
     1081 tcgcgttcgt aaattacacg acgctcggtt acggcgacgt aacgcccgtg gagcgctggc
     1141 agttgctggg accgatgacg gcgatgaacg gcgttctgct gttcggctgg tcgaccgccg
     1201 tcattttcga agtgctgcgg agaacggtga gactcggggc cgcggcaacg tccaatatga
     1261 aagaacggta ggggttgagc cggccgatcg ctggcctcgc tcatgatcca taggccgccg
     1321 gtggcccttc ccgtcaggtg cggagctgcg cgtccactgc aagcaggtat cggcccatgc
     1381 cacagtcgtc cgggctgttg agaaagcgcg tcgccggcgc cttgttgatg gcgctgggag
     1441 ctgtggctct ggctgcccca atggcagcgg gccgctggtc gcttgcgatc ctcggcattc
     1501 ccctgatcg
//
LOCUS       DATAOZ010000464         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4660830, whole genome shotgun sequence.
ACCESSION   DATAOZ010000464 DATAOZ010000000
VERSION     DATAOZ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4660830"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VHR64_11820"
     CDS             <1..712
                     /locus_tag="VHR64_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303562.1"
                     /translation="CDYAIRRGAETTDDAKTILRRESPVALLVETEIGSRLRRLINDL
                     GIGSRAPESFARRRRGHQLAGRVISDPALSFKQRSVDQDIGDNALSSLQVHAEGEEDQ
                     AVASGDIGERWAVDIGLPADASLELIHAVEREVAAIPSFLRGITSYASSDGIVVGWPH
                     GHAPEPQEIAVVIQAWTKALFDLPTASVRVIFAPPTGESEHLTELRERADTIRMYRNA
                     MRSDADPSSVSTKGNPGG"
     gene            713..1540
                     /locus_tag="VHR64_11825"
     CDS             713..1540
                     /locus_tag="VHR64_11825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX3303563.1"
                     /translation="MSSEQDAIQRLRSATGWIFDMDGVLYRGTEALPGVQELFDELHH
                     RGLPFRLATNNSMASPTMYVDRLAGMGIAADEGSILTSALATRQYILDHIGSTAKLHV
                     LGMPALTDQLLANGDFTVVDPDSETPDAVVVGLDRDVTYDKLRKADRGIRYGAQFIAT
                     NGDVTLPTENGLVPGCGALVAALVASTGRHPVVIGKPEAHLLEAAVADMGIRRQQCVM
                     VGDRLDTDIAAGHSLGMLTVMVLTGVSTREEIASAPIKPDLVYTDLPAVLESLLHEG"
     gene            1546..>1989
                     /locus_tag="VHR64_11830"
     CDS             1546..>1989
                     /locus_tag="VHR64_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX3303564.1"
                     /translation="MRLTDDISLVGGGNTGFNLSAPLDCHIYLINGGNGELALVDAGM
                     GGKYGETERIIDNIRRDGYDLNRITTLILTHYHADHSGGTWDWGTRLPNLRVIGSALT
                     ARVMQEGDEAAISLPEARDGGMYPQDYTLTKWQCEPDLIDSRAFQF"
BASE COUNT          387 a          603 c          630 g          369 t
ORIGIN      
        1 gtgcgactac gcgattcggc gtggagcgga gacgaccgac gacgctaaga caatccttcg
       61 ccgggagtcg ccagtcgcgc tgcttgtcga aacggaaatc ggttcccgac tgcggagatt
      121 gatcaacgac ctgggtatcg gaagtcgagc tccggaatcc ttcgcgcgtc ggcggcgtgg
      181 gcaccagctc gcaggcagag tgatctccga tcccgcactt tccttcaagc agcgatcggt
      241 cgatcaggac atcggcgaca acgcgctctc gtcgttgcaa gtccatgctg agggtgaaga
      301 agaccaggca gtcgcctcgg gcgatatcgg cgagcgatgg gctgtcgata taggccttcc
      361 tgccgacgcc tcgctcgaat tgatccacgc agtcgagcgc gaagtggctg ccattccctc
      421 gttcctgcgg ggaatcacga gctatgcgag cagtgatggc atcgtagttg gatggccaca
      481 tggccatgcg cccgagccac aggagatcgc cgtcgtcatc caggcgtgga ccaaggccct
      541 cttcgattta ccgaccgcga gcgttcgggt catctttgcc ccgcctaccg gcgaatcgga
      601 gcatctgacc gaattgcggg aacgcgccga tacgattcgg atgtatcgta acgcaatgcg
      661 ctccgacgcg gacccgtctt cagtatctac caagggaaac ccgggagggt aaatgtcttc
      721 tgagcaggat gcgatccagc gattgcgcag cgcaaccggc tggattttcg acatggacgg
      781 cgtgctctat cggggaactg aggcactgcc cggcgtgcag gaattgtttg acgagctgca
      841 tcaccgcggg ctgccgtttc gcctcgcgac gaacaattcg atggcatcgc cgaccatgta
      901 cgtcgatcgg ctggccggaa tgggaatcgc ggcggacgaa ggttcgatcc tgacctcagc
      961 cttggcaacg cgccagtaca tcctcgacca catcggctcg acggcgaaac tgcacgtact
     1021 gggcatgccg gcactgaccg atcagttgct cgcgaatggc gacttcacgg tcgtcgatcc
     1081 cgactccgag acgccggacg cggtcgtggt tggcctcgat cgcgacgtca cgtatgacaa
     1141 gctgcgcaag gccgaccgcg gtattcgata cggcgcgcaa ttcatcgcca ccaatggtga
     1201 cgtgaccttg ccgacggaga acggtctcgt gcccgggtgc ggagcgctgg ttgccgcgct
     1261 ggttgcgtcg accggtcgtc atccggtcgt aatcggaaag ccggaggcgc acctgctcga
     1321 ggcggctgtt gccgacatgg gaatcaggcg gcagcaatgc gtgatggtcg gcgatcgcct
     1381 cgataccgat attgccgcgg gccactcgct cggcatgttg accgtgatgg tactgaccgg
     1441 cgtgtcgacc cgcgaggaga tcgcttcggc gccgatcaag ccggatctgg tctacacgga
     1501 cttgcctgcg gtgcttgaat cactgctcca cgaggggtaa acgagatgcg actgacagac
     1561 gacatttcac tcgttggcgg aggcaatact ggcttcaacc tctcggcccc gctcgattgc
     1621 cacatctatc tgatcaacgg cgggaacggc gaactcgccc tggttgatgc cggaatgggc
     1681 ggcaaatacg gcgagaccga gcggatcatc gacaacatcc ggcgcgacgg ttacgacctg
     1741 aaccggatca cgaccctgat ccttacccac taccacgccg atcactcggg cggaacatgg
     1801 gattggggaa cacgcctgcc caatttgcgg gtgatcggct cggcgttgac cgcgcgggtc
     1861 atgcaggaag gcgacgaggc ggcgatttcg ctccccgagg cccgtgacgg cggtatgtac
     1921 ccacaggact acaccctcac aaagtggcag tgcgagccgg atttgatcga ctcgcgcgcg
     1981 ttccagttc
//
LOCUS       DATAOZ010000465         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9132526, whole genome shotgun sequence.
ACCESSION   DATAOZ010000465 DATAOZ010000000
VERSION     DATAOZ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9132526"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..380)
                     /locus_tag="VHR64_11835"
     CDS             complement(156..380)
                     /locus_tag="VHR64_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303565.1"
                     /translation="MSSRTGAVLVSRCICRGVAFDELLPRARASNWSLIDLIGETGCG
                     GQCGLCRPYLREMLRTGQTAFTELLPPESA"
     gene            complement(412..1116)
                     /locus_tag="VHR64_11840"
     CDS             complement(412..1116)
                     /locus_tag="VHR64_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3303566.1"
                     /translation="MSKTALITGGTEGIGRATAFALGREGYRVGVCSRSEAKVKRVLE
                     DLAKEGIEAAGAAGDVGDQAQADRVSRKLIGELGKIDVLVNNAGVLIAKPFEELSLDD
                     WDRTMASNVRSLFLMTRAVLPAMRSRKQGAIVNVASLAGRNGFVGGTAYTASKHAVLG
                     FSRSLMLETRKDNIRVIAICPGSVATSMMRDQPMLKADPQKVLQPEDVAASIVHALGL
                     PARAMVSELDIRPTNP"
     gene            complement(1196..>1666)
                     /locus_tag="VHR64_11845"
     CDS             complement(1196..>1666)
                     /locus_tag="VHR64_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303567.1"
                     /translation="LSTSTPMGRRARPRMLSHAAPLIRVKSKDLARAGVVRVPRTAGV
                     RNGRPLLDDGQVLDVTNVVWCTGYEPGLSWLDLPVFDPEGRPLHRRGISIAEPGLSFV
                     GLHFLHAMSSAMIHGVGRDAEYIAERIASSRRPATADRSSLRDTEFFPGEAVRR"
BASE COUNT          298 a          557 c          532 g          279 t
ORIGIN      
        1 cagaccgacg gcgtcgtccc tccggatcag ccagagaccg agccagctga tgatgatcgc
       61 gacgccggcc accgagccgg tgtaggacag cttgcgccaa cggcgctcga gaaaatcgcc
      121 gatgctccgg gtgacagata cttcctgcgg agtgctcagg cgctctccgg cgggagcagc
      181 tcggtgaacg cggtctggcc ggttcgcagc atctcccgca gataggggcg acacaggccg
      241 cactggccgc cgcagccggt ctccccgatg agatcgatga gactccagtt cgatgctctg
      301 gcccggggca gcagctcgtc gaacgccacg ccacggcaga tgcagcggga tacgagcacc
      361 gctccggttc ggctactcat ggcgaccgcc tgaccgcgtg accgcctgct gtcatggatt
      421 cgtcggcctg atgtcgagct cgctcaccat cgcgcgcgcc ggtaagccga gcgcgtgtac
      481 gatcgaggcg gcgacatctt caggctggag gactttctga ggatcggctt tgagcatcgg
      541 ctggtcacgc atcatgctgg tggcgacgga tccggggcag atcgcgatca cccgtatgtt
      601 gtccttcctg gtctccagca tgagcgagcg gctgaatccc agtactgcgt gcttcgatgc
      661 cgtataggcg gtaccgccga cgaagccgtt gcgaccggca agactggcca cgttcacgat
      721 ggcgccctgc ttccgactcc gcatggccgg cagcactgcc cgggtcatca ggaagaggct
      781 gcggacgttg ctcgccatcg tccggtccca gtcatccaag ctcaactcct cgaacggctt
      841 ggcgatgagg accccggcgt tgttcaccag gacgtcgatc ttgcccaact cgccgatcag
      901 cttgcgactg acccggtcgg cctgagcctg gtcaccaacg tctcccgccg cgcctgcagc
      961 ttctatgccc tccttcgcca ggtcttcgag gacgcgtttc accttggcct ctgagcggga
     1021 gcacaccccg acacggtagc cctcgcgccc cagcgcgaac gccgtcgctc ggccgatgcc
     1081 ctcggtgccg ccggtgatga gagcggtctt cgacatgggg ggcaatgtag gcggtaaggc
     1141 agaacggcgg aaaggcggta gaaacggtaa gcatgcgctg agcgcagcga aggggctacc
     1201 tccttaccgc ctcgccggga aagaactcgg tgtctcgcaa ggaagaccgg tccgctgtgg
     1261 ccggacgcct ggaggaagcg atccgctcgg cgatatactc ggcatcccgg ccaacgccat
     1321 ggatcatggc cgaagacatc gcatggagaa aatggagacc gacaaagctg agtcccggct
     1381 cggctattga gatgccccgc cggtggagtg gtcgtccttc cggatcgaac accggcaaat
     1441 cgagccagga gaggcccggc tcgtaacccg tgcaccatac cacgttggtg acatcgagaa
     1501 cctggccatc atccagcagc ggccggccgt tgcgtacgcc cgccgttctc ggcacccgta
     1561 ccacacccgc acgagcaaga tccttcgact tgacccggat cagcggtgcc gcgtgcgaca
     1621 gcatccgcgg gcgggccctt cgccccatcg gggtgctggt gctcag
//
LOCUS       DATAOZ010000466         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8607642, whole genome shotgun sequence.
ACCESSION   DATAOZ010000466 DATAOZ010000000
VERSION     DATAOZ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8607642"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VHR64_11850"
     CDS             complement(<1..1458)
                     /locus_tag="VHR64_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3303568.1"
                     /translation="MAFIMNGLDAESYDRKYTDRQLINRIKTYFRPESRKIGLVTIMV
                     VLNSMLDAALPILVATWLDRIVGPNGVDSRVWWLVAAIILFGAAAWGAYFVRQSKSAE
                     IVGNIILRLRTDAIDAVMKRDMSFFDEYSSGRVVSRVTSDTQDFSNTVTLVMALFSQV
                     LLVFIVTAVLFVRSPQLTLLVIVLIPIIIAIALGFRKVARQVTIQSQRVQGEVNGMIQ
                     ETMRGIAVAKSYRQEATIYREFEEVSDRTYRIRLKQGFIFSGIFPLLFAVAGLGTTAL
                     VWVGGNQVIDGNISAGDWYLFLQAVGLFWFPLTSIASFWSQFQQGLSAAERVFALIDA
                     EARVTQTDDVEPARLSGKIEFRDVTFGYKDERPVLENFSLTIAPGETIALVGHTGAGK
                     STLGRLISRFYEFQGGQILIDDQDIRTFDLGAYRRQLGIVPQLPFLFSGTVADNIRYA
                     LPDATPEQIDYAASHIGQGDWVDALQEGLETPIGEE"
     gene            complement(1458..>1730)
                     /locus_tag="VHR64_11855"
     CDS             complement(1458..>1730)
                     /locus_tag="VHR64_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3303569.1"
                     /translation="DDSTSAIDSATEDQIQRAIKAISADRTTILITHRLSQIRWADKV
                     LVMRHGEVIDFGTHEELLGRCHLYLRIFARYDEAPERQAVTAGEAD"
BASE COUNT          319 a          550 c          551 g          310 t
ORIGIN      
        1 ctcctcgccg atgggcgtct cgaggccctc ctggagcgcg tcgacccagt cgccttggcc
       61 aatgtgcgaa gcggcgtagt cgatctgctc cggcgtcgcg tccggcaagg cgtatcggat
      121 attgtcggca acggtgccgg agaagaggaa cgggagctgc gggacgatcc cgagttgccg
      181 ccggtacgcc ccgaggtcga aggtcctgat gtcctgatcg tcgatcagga tctgcccgcc
      241 ctggaactcg tagaagcgcg agatcagccg gccgagggtc gatttgcccg cgccggtgtg
      301 cccgacaagc gcaatcgtct cgcccggcgc gatcgtcagc gagaagttct cgaggaccgg
      361 ccgctcgtcc ttgtatccga acgtgacgtc ccggaactcg atcttgccgg acaggcgcgc
      421 tggctcgacg tcgtcggtct gagttactcg cgcctcggca tcgattagcg cgaagacccg
      481 ctcggccgcc gagaggccct gctggaactg gctccagaac gacgcgatgc tggtcagcgg
      541 gaaccagaac aaccccacgg cctgcaggaa gaggtaccag tcgccggccg agatattgcc
      601 gtcgatcacc tggttgccgc cgacccagac cagcgcggtc gtgccaagcc cggccaccgc
      661 gaagagaagc ggaaaaatgc cgctgaagat aaagccctgc ttgagccgga tccggtaggt
      721 gcggtcgctg acctcttcga actcgcggta gatcgtcgcc tcctgccggt agctcttggc
      781 gacggcgatc ccgcgcatcg tctcctgaat catgccgttg acttcgccct ggacccgctg
      841 tgactggatg gtgacctgcc gcgccacctt ccggaaaccg agcgcaattg cgatgatgat
      901 cggaatcagg acgatcacga gcagggtcaa ctgcgggctg cgcacgaaga gcaccgcggt
      961 cacgataaag acgagcagta cctggctgaa gagcgccatc accaatgtga cggtgttcga
     1021 gaagtcctgc gtgtccgatg tcacccggct tacgaccctg cccgatgagt attcatcgaa
     1081 gaaggacatg tcccgcttca tgaccgcgtc gatcgcatca gtccggagcc gaaggatgat
     1141 gttgccgacg atctcggccg acttcgactg gcggacgaag taggcgcccc aggccgccgc
     1201 gccgaagagg atgatcgccg ccaccagcca ccagacccgg ctgtcgacgc cgtttggccc
     1261 gacgatccgg tccagccagg ttgcgaccag gatcggcaac gcagcatcca gcatcgagtt
     1321 gagcacgacc atgatcgtga ccaggccgat cttgcgggac tcaggccgga aataggtctt
     1381 gatccggttg atgagttggc gatcggtgta tttgcggtcg tacgactcgg cgtcgagacc
     1441 gttcatgatg aatgccatca gtcggcctcc cctgccgtga ctgcttgccg ctccggcgcc
     1501 tcgtcatacc tggcgaagat gcgaaggtac agatggcagc ggccgagcag ctcctcgtgc
     1561 gtgccgaagt cgatcacctc gccgtggcgc atgaccagaa ccttgtccgc ccagcggatc
     1621 tggctcagtc gatgcgtgat caggatcgta gtgcggtccg cgctgatcgc cttgatcgcg
     1681 cgctggatct gatcctcggt cgcactgtcg atcgcgctgg tcgagtcgtc
//
LOCUS       DATAOZ010000467         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1502947, whole genome shotgun sequence.
ACCESSION   DATAOZ010000467 DATAOZ010000000
VERSION     DATAOZ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502947"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VHR64_11860"
     CDS             complement(<1..309)
                     /locus_tag="VHR64_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX3303570.1"
                     /translation="MKQQRLFILIGAAAAATVGWCGLAVAQTGQGLEPLMAKSSNWAT
                     QAGDYANHRYSDLKQVNASNVGQLQVAWTMSTGVLRGHEGSPLVIGDTLYLHTPFPNN
                     V"
     gene            complement(540..>1613)
                     /locus_tag="VHR64_11865"
     CDS             complement(540..>1613)
                     /locus_tag="VHR64_11865"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEX3303571.1"
                     /translation="KLNADGSQLFVAVSGLPKCPPSVPDEECAKLERDLKADGIAVVD
                     TATQKVVKVLHAGSDPEQFALSRDGKRLFVANEDSATLSVVDIKSGTVVQRVPVGREP
                     EGVAVTPDGRWVLVTNESDNSVSIIDTRTLKIVKSVQVGKRPRDVAFTPDGRTAYVSG
                     EFDASVYRMTLPQGEPVERVMELRTEARPMGILLDSGRNRLYVSTGRGGTVAVIDSTT
                     NKLLAEVKVGTRPWGIALSKDGRWLYTANGPSDDISVIDTSTLSAVRNIRVGRSPWGV
                     AFGPEPPAGQRSGEAESSRSQSAATVRLGLGFPIALGHLLRGVGVAEDPAAFTREGRV
                     FRVGRGLHEFVVAQGIGDAVDGA"
BASE COUNT          288 a          549 c          480 g          296 t
ORIGIN      
        1 cacgttgttc ggaaacggcg tatgcaggta caacgtatcg ccaatcacga gcggcgagcc
       61 ttcgtggcct cgcaacactc ccgtcgacat cgtccatgcc acctgcagtt gcccgacgtt
      121 gctggcgttg acctgcttga gatcgctgta acgatggttg gcgtagtcgc cggcctgcgt
      181 cgcccagttg gacgatttgg ccatgagcgg ctcgagacct tgtcccgtct gggcgaccgc
      241 caggccgcac cagcccaccg tcgcggcggc cgccgcaccg attagaatga agagacgctg
      301 ctgtttcatg tttcaatccc ccgtcagccg ctacgaatcg agtgacactc acgatgacaa
      361 tgcaacgcct atgccaaatg cccgcattgc gggccctgct ctgcacggcg acgctcggca
      421 tcagctgcgc ggtcggcgcc gactcgcccg tcaacgatta cccgacggtg gcgcgcgtgg
      481 agttcgttca ggaatgcatc gccacgcacg gcggcaagct cgagaacctc tatcagtgct
      541 catgcgccat cgaccgcatc gccaatgccc tgagctacga cgaattcgtg gaggcctcga
      601 cctacgcgaa atactcgacc ctcccgggtg aaggcggcgg gatcttccgc gactccgaca
      661 ccgcgaagca gatggccaag cgctatcggg aaaccgaggc cgaggcgtac cgtagctgcg
      721 gattgggatc ttgagctctc ggcttcgccc gatcgttgcc ctgccggcgg ctcaggcccg
      781 aacgcaactc cccagggcga gcgcccgacg cgaatattcc gaaccgcgct cagcgtcgat
      841 gtatcgatca cactgatgtc gtccgatggg ccgttggccg tgtagagcca gcgaccgtcc
      901 ttggacaacg cgatgcccca cggccgcgtg ccgaccttca cctcagcgag cagcttgttg
      961 gtcgtgctgt cgatgaccgc aaccgtgccg ccgcggcccg tgctcacgta aagccgattg
     1021 cggcctgaat ccagcaggat tcccataggg cgcgcctccg tgcgcaactc catcacacgc
     1081 tcgaccggct cgccctgcgg cagcgtcatg cgatagaccg aggcgtcgaa ctcaccggat
     1141 acataggccg tgcgaccgtc cggcgtgaag gcgacatcgc gcggacgctt gccgacctgc
     1201 accgacttga cgatcttcag ggtgcgggtg tcgatgatcg acaccgagtt gtcggactcg
     1261 ttggtgacca gaacccagcg tccatccggc gtgacggcga cgccttcagg ctcacgccct
     1321 acaggcaccc tctgcacgac cgtgccactt ttaatgtcta cgactgacaa ggtggccgag
     1381 tcttcgttgg ccacgaacag ccttttgcca tcccggctga gcgcgaactg ctccgggtcg
     1441 gagccggcgt gtaacacttt cacgactttt tgcgttgccg tatcaaccac ggcaatgccg
     1501 tcggccttca ggtcccgctc gagcttcgcg cactcctcgt ccgggaccga cggcgggcac
     1561 ttgggcaggc cgctgactgc gacgaacagc tgcgatccgt cggcattgag ctt
//
LOCUS       DATAOZ010000468         8647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7819051, whole genome shotgun sequence.
ACCESSION   DATAOZ010000468 DATAOZ010000000
VERSION     DATAOZ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7819051"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VHR64_11870"
     CDS             complement(<1..620)
                     /locus_tag="VHR64_11870"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3303572.1"
                     /translation="MAADRLLVNARVLTMAGDRVAEAVAIADGRILAVGSDAEMRALS
                     GPETVVEDLGGATVMPGLIDAHNHLLMTGQILQQVQLYDCRSVDDILDRVRTAVEKAP
                     PGTWILGRGWDESLLAERRHPTRHDLDRVAPDHPVVIHRVWNRLVANSRAIALAGVSR
                     ETPDPPADQPYAGGFERDADGEPTGLFRDRAKELITSAVPAPTLDEL"
     gene            complement(694..1476)
                     /locus_tag="VHR64_11875"
     CDS             complement(694..1476)
                     /locus_tag="VHR64_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX3303573.1"
                     /translation="MASESQGVLAGKNVLIMGVANRWSIAYAIGEAMKAQGATLILSY
                     LDERMKKDCEALLSDQPNGKMYPCDVSKDEDIDALAESIKSDFGRLDTYLHSVGFAPG
                     DAMKNSFLETTRDGWRIALDVSAYSLVAVSARIAPLMTDGGSIQTLTYLGSERVFPAY
                     KVMGVAKAALEASVRYLAWDLGEQGIRVNAISAGPIKTAAARGIPGFMDMYKHLNEKT
                     PLKSDFTAEKVAGLSVFLASDASAAITGEVIFADNGYHAMGM"
     gene            1544..2611
                     /locus_tag="VHR64_11880"
     CDS             1544..2611
                     /locus_tag="VHR64_11880"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX3303574.1"
                     /translation="MSIQKPMYPVFDGHNDTITHLREAGGYFLEENDDFHITLPLAKR
                     GGLIGGFFAIWIPDADEVPNFTDPNTDSYRSIETMPPMMSTGHAQEFALTSLGRLLRL
                     ERESEGAVRIVRTVADIERAMADGAFSVEVHIEGAEPIDSDLDTLDAWYAAGLRSLGL
                     VWSRPNLFGHGVPFDFPSSPDTGPGLTDAGKALVTACNQLGIMLDISHLNEKGFWDLA
                     GISDRPIVATHCGAHALCPSPRNLTDKQLDAIKDSDGIVGINFHVGFLNPLGNEATAE
                     NTSLTAIADHLDYIADRIGIDRVALGSDFDGARMPGDLANAGGLPKLMAELKSRGYDD
                     NALRKIGYENWLRVFRATWVA"
     gene            complement(2709..3062)
                     /gene="rpsR"
                     /locus_tag="VHR64_11885"
     CDS             complement(2709..3062)
                     /gene="rpsR"
                     /locus_tag="VHR64_11885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX3303575.1"
                     /translation="MDNEQTNQATESAPAERSRFGQGAAGERPSRPPRDRRDGGPRGG
                     RGGGRRKQPVFADNEVVDYKDVQRLKMCLDERGKILPRRKTHCSAGKQRELTTAIKRA
                     RHLALLHFVPSDRRR"
     gene            complement(3163..4044)
                     /locus_tag="VHR64_11890"
     CDS             complement(3163..4044)
                     /locus_tag="VHR64_11890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX3303576.1"
                     /translation="MKLVLHFNDFSWPLASDQLGATLVEVARASEAAGFDSIAVMDHL
                     WQHPMMGGPEKPVLECYAALSFIAAHTSRVGLVALATAASYRPAGLLAKIVTSLDVLS
                     GGRAWLGIGAGDYEEEARGLGIPYPSLAERFVILEETIQVCLQMWSGEHGSDQPFQGE
                     HVHLERTLNLPQSLSRPHPPILIAGSGEQRTLRLVARYGDACNIRPGPEIPHKLEVLR
                     RHCEAEGRNYDEIERTCPFLFDVGEDGSKTGELVEQLRGLAQMGIQKVFGRVVGDYRI
                     EPIEIMGRDVIPAVAEL"
     gene            4336..4527
                     /locus_tag="VHR64_11895"
                     /pseudo
     CDS             4336..4527
                     /locus_tag="VHR64_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
     gene            4633..4827
                     /locus_tag="VHR64_11900"
     CDS             4633..4827
                     /locus_tag="VHR64_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX3303577.1"
                     /translation="MTGVVKRYDAAKGFGFITPDDGGHDVFVHRSACGYGGLVEGDHV
                     EFRLGDGPKGPRAEQVRVVD"
     gene            5031..5549
                     /locus_tag="VHR64_11905"
     CDS             5031..5549
                     /locus_tag="VHR64_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303578.1"
                     /translation="MVAQNAPDAENSKVIVFCERHTMLAELNTGQRRLSDVINDPLHK
                     LFHLEQVRINRGDRMDENVAEYPAVVIKREAVQALMVMGEPNRPPQQRISNYVPKQPI
                     RVAALLPSFHIVGQIFPGKVDAIEFLLNGSETFAVLRNATVTMTTRVDKPITVPTAFL
                     NRSHIELATAVQ"
     gene            complement(5543..6079)
                     /locus_tag="VHR64_11910"
     CDS             complement(5543..6079)
                     /locus_tag="VHR64_11910"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX3303579.1"
                     /translation="MTQPTTSDELELLKRHIRDVPDFPQPGVLFRDITPMLADPESFS
                     LAVDALARPYSGIDKVVAIESRGFILGAPVALALHAGLVPVRKVGRLPADTLREDYSL
                     EYGVNVVEIHKDAIQSGERVLIVDDVLATGGTVRAAANLVQRLGGIVVGVSLLVELSF
                     LGGRDRLQDLDLHTVLVY"
     gene            6321..7256
                     /locus_tag="VHR64_11915"
     CDS             6321..7256
                     /locus_tag="VHR64_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX3303580.1"
                     /translation="MTPTTRRVALKGLIGASAMAHAVKTQFASAQDSEDPTPATFKKP
                     QTLIVGGLDSRQQGEPENSDVLMLARVDLAAKLVRVISIPRDLYIDIPGFGADKITRA
                     YDFGSKSQNGKFKAGAELMKATIEQSFGLSIDGVVLTTFHGFEHVVDAFGGVDVNNPY
                     DVADDQYPTLDYGYMSIFFPAGEQTLTGEQALQFCRTRHQDGDPGRVMRQQLVLRGLL
                     DKATSPDYSSQLWDIVKEKKKEVRTDLGPSKQLAFALAVPDFTNDNVSFATLDDYVYA
                     DSAPNGMWIYSGVWDQIPTYVEQFLAGQIQASPSP"
     gene            7352..>8647
                     /gene="tyrS"
                     /locus_tag="VHR64_11920"
     CDS             7352..>8647
                     /gene="tyrS"
                     /locus_tag="VHR64_11920"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX3303581.1"
                     /translation="MTAPSVDAALRARERGVHLIDYLRSRGYVQDISDEAGLRETLDR
                     EVVTGYIGFDPTAASLHVGHLLSIMVLGTLQRFGHRPIALAGGGTTMVGDPTGKTATR
                     KVLAPEEIQTNLQNVVKQFDRYIDFKGGRFGDRSPAALLVNNADWLLQLKYIEFLRDI
                     GRHFSVNEMLAAETYRQRLETTGLNFVEFNYRIVQAYDFLHLHRTVGCKLQMGGSDQW
                     GNIVAGVDLIRRVEGGHAFALVTPLLTTASGQKMGKSEGNSLWLDPTMTTPFDYYQYW
                     INTDDADVRKLLMLYTFLPEELIDELTSVEGEALREAKRLLAFESTRLTHGLEAAREA
                     QEAATRLFSGSRTSSDLLADEGIPSVEIPRAEIDGMSIADLFVRAGLSSSRSDARRTA
                     AQGGLSIGDARLASVDDPVPIDGSALLLRAGKKRFIRVLL"
BASE COUNT         1598 a         2799 c         2690 g         1560 t
ORIGIN      
        1 agctcgtcaa gcgtcggcgc gggcacggcc gaggtgatca actccttggc ccggtcgcgg
       61 aacaggccgg tcggctcgcc gtcggcgtcg cgctcgaatc cgcccgcata gggctggtcg
      121 gctggcggat cgggcgtctc ccgcgagacc ccggccagcg cgatcgcccg cgaattggcg
      181 accagccggt tccagacgcg gtgaattacg accgggtgat ccggcgcgac ccgatcgagg
      241 tcgtgacggg taggatgccg gcgctccgcc aggagactct catcccagcc gcgacccaga
      301 atccaggtgc cgggcggcgc cttctcgaca gccgtgcgga cgcgatcgag gatatcgtcg
      361 acgctgcggc agtcgtacag ctggacctgc tgcaggatct ggccggtcat cagcaggtgg
      421 ttgtgggcat cgatcagccc tggcatgacg gtcgcgccgc cgagatcctc gacaacggtc
      481 tccggtccag acagcgcccg catctccgca tccgacccca ccgccaaaat ccggccgtcc
      541 gcaatcgcga ccgcctcagc aacccgatcg ccagccatcg tcaagacacg cgcattcaca
      601 agcaaacgat ctgccgccat tggtttccag tttctcgttt ctgattctcg ttctgacgtc
      661 tgaagtccgg tgtctgtcga ctgtcctctg tcgtcacatt cccattgcgt ggtagccgtt
      721 gtcggcgaag atgacctcgc cggtgattgc ggcggaggca tcgctggcca ggaagacgga
      781 aagacccgcc accttctcgg ccgtgaaatc ggacttgagc ggggtcttct cattgagatg
      841 cttgtacatg tccatgaagc cggggatgcc gcgcgcggcg gccgtcttga tcggtccggc
      901 cgagatggcg ttgacccgga tgccctgctc gcccaggtcc caggccaggt atctgaccga
      961 tgcctcgagc gccgccttgg ccacgcccat caccttgtag gccgggaaga cacgctcgga
     1021 accgaggtac gtcagggtct ggatgctgcc gccatcggtc atcagcgggg caattcgggc
     1081 cgagaccgcg accagcgagt aggcgctgac gtcgagcgcg atccgccaac cgtctcgcgt
     1141 cgtctccagg aacgagttct tcatcgcgtc gccgggcgca aatccgacgc tgtggaggta
     1201 ggtatcgagg cggccgaaat cggacttgat cgactcagcg agggcgtcga tatcctcgtc
     1261 cttcgagaca tcgcaggggt acatcttgcc gttcggctga tcggagagca gggcctcgca
     1321 atccttcttc atgcgctcat cgaggtagct gaggatgagc gtcgcgccct gtgccttcat
     1381 cgcctcgccg atcgcgtagg cgatgctcca gcgattcgcc acgcccataa tcagcacgtt
     1441 tttgccggcc aggacgccct gcgattctga tgccattcgg tgattccctc cgctgcggta
     1501 gagtgacggc gttcggcaga tgatactcgt ccgaggagtc accttgagca tccagaagcc
     1561 catgtacccg gtattcgatg gccacaacga cacgatcacg cacttgcggg aggcaggcgg
     1621 atacttcttg gaggagaacg acgacttcca tatcactctg ccgttggcga agcgcggcgg
     1681 actgatcggc ggcttcttcg cgatctggat tccggacgct gacgaggtcc cgaatttcac
     1741 cgatccgaat accgactcct accgcagcat cgagacgatg ccgccgatga tgtcgaccgg
     1801 ccacgcgcag gaattcgcgc tgacctcgct cggccggttg ctgcgcctcg agcgcgagag
     1861 cgagggcgcg gtgcgcatcg tccggaccgt cgccgacatc gagcgggcga tggccgacgg
     1921 cgccttttcg gtcgaggtcc atatcgaggg cgcggagccg atcgactccg atctcgacac
     1981 gctcgatgct tggtatgcgg ccgggttgcg ctcgctcggg ctggtctggt cgcgcccgaa
     2041 ccttttcggc catggcgtcc cgttcgactt cccgtcgtcg ccggacaccg gtcccggcct
     2101 gaccgatgcc ggcaaggcgc tggtcacggc ctgcaaccag ctcgggatca tgctcgatat
     2161 ctcgcacctg aatgagaagg ggttctggga tctggccggg atcagcgatc ggccgattgt
     2221 cgcgacgcac tgcggtgcgc acgcgctttg cccgtcgcca cggaacctga ccgacaagca
     2281 gctcgatgcc attaaggact cggacggaat cgttgggatt aacttccacg tcggcttcct
     2341 gaacccgctc ggcaacgaag cgaccgccga gaacacgtcg ctgaccgcga tcgccgatca
     2401 cctcgattac atcgcggatc ggatcgggat tgatcgggtc gcgctcggct ccgacttcga
     2461 cggcgcgcgc atgccgggcg acctcgcaaa tgcagggggt ctgccgaagc tgatggcgga
     2521 gttgaagtcg cgcggctacg acgacaatgc attgcgcaag atcggctacg agaactggct
     2581 ccgcgtcttc cgcgccacgt gggtcgcgta agcaacgggc tcatcttcgt ttggtggaat
     2641 cgatcaagaa gagaaaagcg ggccccggat aatcccggag cccgctttcg gttctatatc
     2701 gtccgcgctt atcggcggcg gtcgctcggc acgaagtgca ggagggcgag gtgccgggcc
     2761 cgcttgatcg cggtcgtcag ctcgcgctgc ttgcctgcgg agcaatgcgt cttgcggcgc
     2821 ggcagaatct tgccccgctc atcaaggcac atcttgagac gctggacgtc cttgtagtcg
     2881 acaacctcgt tatcggcgaa gactggctgc ttgcggcggc cgccaccacg gcctccgcgc
     2941 ggcccgccat cccggcgatc tcgcggcggt cgagacggac gttccccagc ggcgccttgt
     3001 ccgaagcgcg agcgctcggc aggagcgctc tcggtcgcct ggttcgtctg ctcgttgtcc
     3061 atacctgtaa acgtttctcc ttgtgcatag gcacgataac ccgcggatga tagcatagcc
     3121 gaccgtctca gcgacctgac agcagtccca aacaccggct cgctagagtt cggcaaccgc
     3181 cggaatcacg tcgcgaccca tgatctcgat cggctcgatc cggtagtccc cgaccacccg
     3241 gccgaatacc ttctgaatac ccatctgtgc cagtccgcgc agctgctcga ccagctcccc
     3301 ggtcttcgag ccgtcctcgc cgacatcgaa gaggaagggg caggtccgct cgatctcgtc
     3361 gtagttgcgg ccctcggcct cgcaatggcg tcggagcacc tcgagcttgt gcggtatctc
     3421 cggacccggc cggatgttgc aggcatcgcc gtaccgtgcg acaaggcgca acgtgcgttg
     3481 ttccccactc ccggcaatca ggatcggcgg atgtggccgg ctcaggctct ggggcaggtt
     3541 gagcgtgcgc tccaggtgaa cgtgctcgcc ctggaacggc tggtcggagc catgctcgcc
     3601 gctccacatc tgcaggcaga cctggatcgt ctcttccaga atcacgaaac gctcggcgag
     3661 cgacggatac gggataccga gaccacgtgc ttcctcttca taatcccccg cgccaatacc
     3721 gagccaggcc cggccgccag agagcacatc gagactggtg acgatcttgg ccagcaaacc
     3781 ggccgggcgg tacgaggcag cagtggcaag ggccacgagg ccgacgcggc tggtgtgcgc
     3841 ggcgatgaat gagagtgccg catagcactc gagcaccggt ttctccggtc cgcccatcat
     3901 tggatgctgc cagagatgat ccatgaccgc gatcgagtcg aatcccgccg cttcgctggc
     3961 ccgggcgacc tccacaagcg tggcacccaa ctgatctgaa gccagcggcc aggaaaaatc
     4021 gttaaagtga aggacgagct tcatgcggtt tcatgctcct gattgggacc gatgaggggt
     4081 gcgcgaagca cccggagcac gcgctttcgg catacactac acgggtaagc atcggaatcc
     4141 gtgggaatta agtagtgtgg acggcccccg gcggggagga gaacccctgc cattctgagg
     4201 aaaattgccc aaaccccttg cctttttcgg gatttcgctc aatgatatat cgcgtcagcg
     4261 acaatcggct tttcgatcgc aggcagggaa aagggcggcg cgtgccgcta gtagtcagtg
     4321 gagcggtaaa aaatcgtgcc aaccggaacc gttaagtggt ttgacccgga caagggtttt
     4381 ggattcatcg cgcgtgacga tggtggtccg gacctcttcg tccatcggtc agcacttgga
     4441 atgcagaccc ttggtgaggg tgatcgagtc gacttcgtcg ctggtacggg catgaagggc
     4501 ccggccgcca ccaacatcac aatcctcgag cgcaaccccg atccgaagcc gcgccgcgct
     4561 ggcggtatgg gcggcggcgg attcggcggc gactttgggg ggtcgagcgt cgatgtatcg
     4621 aacctcccgc tcatgaccgg tgtcgtcaag cggtacgatg ccgccaaggg cttcggcttt
     4681 attacgccgg atgacggcgg tcatgatgtc ttcgtgcatc gctcggcctg tggctatggc
     4741 ggccttgtcg aaggtgatca cgttgagttc cgcctcggcg acggcccgaa gggcccacgc
     4801 gccgagcagg ttcgagtcgt cgactagcgc ccgaatccca ccacaagacg attacacgct
     4861 cttcgggagt acgcttaggc gtgctcccga tgcttttgtg accgcctaac ccggacttct
     4921 cccccatgtc gggccctata cggacatggc ccaaacgctc tatcgtctct gcgattccgc
     4981 tggtgtggcg gatcacgacg gcaaatgcgc agtttcagag acgtggaccc atggttgccc
     5041 aaaacgctcc ggacgccgaa aattcgaaag ttatcgtctt ttgcgagcgg catacgatgc
     5101 tggccgagct caacaccggt cagcgccggc tcagcgatgt gatcaatgac ccgctccaca
     5161 agcttttcca cctcgaacag gtgcgaatca accggggcga ccgcatggat gagaacgtcg
     5221 ccgagtatcc ggccgttgtc atcaagcgcg aagcggtaca ggcgctgatg gtgatgggcg
     5281 agccgaatcg ccctccccag caacgaattt cgaactacgt gcccaagcaa ccgattcggg
     5341 tcgccgcact gttgccgtca ttccacatcg tgggccagat ttttcccggc aaggtggacg
     5401 cgatcgaatt cctgctcaac ggctccgaga cgttcgcggt gctcagaaac gcgaccgtca
     5461 cgatgacgac gcgggtcgat aagccaatca ctgtcccgac cgcattcctg aaccgctccc
     5521 atatcgagtt ggcgaccgcc gttcagtaga caagcaccgt gtgcagatcc agatcctgca
     5581 agcggtcccg accgcccaga aagctgagct ctaccagcaa gctgacgccc accacgattc
     5641 cgcccagacg ctggaccaga ttggccgcgg cccggaccgt cccaccggtc gccagcacgt
     5701 catcgacgat cagcaccctc tctccggact ggatcgcatc cttgtggatc tcgaccacgt
     5761 tgacgccgta ctcgagcgag tagtcctcgc ggagcgtatc ggctgggagc cggccgacct
     5821 tccggaccgg gaccaggcca gcgtgcaagg cgagcgccac cggcgcgccg aggataaagc
     5881 cgcgactttc gatcgccaca accttgtcga tgccggaata gggccgcgcc agggcatcga
     5941 cggccagcga aaaggactcg ggatccgcga gcatcggcgt gatatcgcga aacaggacgc
     6001 cgggctgcgg gaaatccggc acgtcccgga tgtgtcgctt gagcagctcg agctcgtcag
     6061 atgtcgtggg ctgagtcatc cggtgggatc ccctcgcgcg atggcactgc gagggtgcca
     6121 tccgattctg ttgatgatcg aaaaacagga tgcccgagcc tgcaagtacg tacagcgcga
     6181 aaccaaccac accgctggcg cgtaggcaat ggggatggta gggtgagagc ccgccccgaa
     6241 gcaatacagt ccggcccagt cctgcgctgc ggccaagaag gtgaggacca acgatcacta
     6301 tgggtttgac acggtttctg atgaccccca cgacgcggcg ggtcgcgctc aagggcttga
     6361 tcggcgcctc agcgatggcg cacgcagtca agacgcaatt cgcatccgcg caggacagcg
     6421 aagatcccac accggcaacg ttcaagaagc ctcaaacgct gatcgtgggc ggtctcgact
     6481 cgcggcagca gggcgagcct gagaacagcg acgtgctgat gctggcccgt gtcgatctcg
     6541 ccgccaagct ggtccgcgtc atcagcattc cgcgcgacct ctacatcgac atccccggtt
     6601 tcggggccga caagatcacc cgcgcctatg atttcggatc gaagtcacag aacggcaagt
     6661 tcaaagccgg cgccgagctg atgaaggcga ccatcgagca gagcttcggg ctcagcatcg
     6721 acggagtggt cctaaccacc ttccacggct tcgagcacgt cgtcgacgca ttcggaggag
     6781 tcgacgtcaa caatccgtac gacgtcgctg acgaccagta cccgacgctc gattacggat
     6841 acatgtcgat cttcttcccg gccggcgagc agacgctaac cggcgagcag gcgctccagt
     6901 tctgccgcac ccgccaccag gacggcgacc ccggccgcgt catgcgccag cagctcgtcc
     6961 tgcgcggcct gctcgacaag gccaccagcc cggactactc cagccaactc tgggacatcg
     7021 tcaaggagaa gaagaaggag gtccggaccg atcttggccc gtccaagcag ctcgcctttg
     7081 ccctggccgt gccggacttc acgaacgaca acgtctcgtt cgcgaccctt gacgactacg
     7141 tctacgcgga ttcggcgcca aacgggatgt ggatctactc cggcgtctgg gatcagatcc
     7201 cgacctacgt cgagcagttc ctcgcgggcc agatccaggc gtcgccatcc ccgtagttga
     7261 caatcgcaaa cccgctcagt agcgtaacga tcggcagaga tcactatctc tgccgatttt
     7321 ttcgtcctca tcgtgaccaa aggcttctgg aatgactgct ccgagtgttg atgccgccct
     7381 tcgtgcccgc gagcgtgggg tgcatctgat cgactatctg cgcagccgtg gatacgtcca
     7441 ggacatctcg gacgaagccg gtctgcggga gaccctcgat cgggaggtgg tcactggtta
     7501 catcgggttc gatccgaccg cggcctcgct ccacgtcggg catctgctca gcatcatggt
     7561 cctcgggacg ctccagcggt tcgggcaccg tccgatcgcg ctggccggag gcggcacgac
     7621 gatggtcggc gatccgaccg gtaagacagc gacgcgcaag gttctcgcgc cggaggagat
     7681 tcagaccaac ctccagaatg tggtcaagca gttcgatcgc tacatcgact tcaagggcgg
     7741 ccgcttcggt gatcgcagcc cggccgcgct gctggtcaac aatgccgact ggctgctcca
     7801 gctcaaatac atcgagttcc tgcgcgatat cggccgccac ttctcggtca acgagatgct
     7861 ggccgccgag acctacaggc aacgcctgga gacgaccggg ctgaacttcg tcgagttcaa
     7921 ctaccggatc gtgcaggcct atgacttcct gcacctccac cggaccgttg gctgcaagct
     7981 gcagatgggt ggctccgatc agtggggcaa tatcgtggcc ggcgtcgatc tgattcgtcg
     8041 ggtcgagggc ggtcatgcct tcgcgctggt tacgccgctg ctgaccacgg cctccgggca
     8101 gaagatgggc aagagcgaag gcaactcgct ctggctcgac ccgaccatga ccacgccgtt
     8161 cgactactac cagtactgga tcaacaccga cgatgcggac gtgcggaagc tgctgatgct
     8221 ttacacgttc ctgccggagg aacttatcga cgagctgacg tcggtcgagg gcgaggcgct
     8281 gcgcgaggcc aagcggctgc tggcgttcga gtcgacccgg ctgacgcacg gcctggaagc
     8341 ggcgcgggag gcgcaggagg cggccacgcg cctcttctcc ggcagccgga ccagctccga
     8401 ccttctggcg gatgagggaa tcccgtcggt cgagattccg cgcgccgaga tcgacggcat
     8461 gtcgatcgcc gacctcttcg tccgggccgg tctcagctcg tcccgcagcg acgcgcgccg
     8521 gacggccgcg caaggcggtc tgtcgatcgg cgacgcgcgt ctggcgtcag tcgacgatcc
     8581 ggtcccgatc gatggctcgg ccctgctcct ccgcgccggc aagaaacgct tcatccgcgt
     8641 cctcctg
//
LOCUS       DATAOZ010000469        11935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8871645, whole genome shotgun sequence.
ACCESSION   DATAOZ010000469 DATAOZ010000000
VERSION     DATAOZ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11935
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8871645"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VHR64_11925"
     CDS             <1..892
                     /locus_tag="VHR64_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3303582.1"
                     /translation="NLASALPQSVHIAPCYRYRYADPRDESYGVTAAMELDRTIRNLG
                     PENVAAFIAEPVVGATLGAVPGERSYFQRIRRICDEHNVLLISDEVMTGFGRTGLAFG
                     IDHWGETPDLITCAKGIAGGYSPLGAVIARSEIVSEVRQRSGSFMIGGTASGNPLSSA
                     TAIAVLNYIVEHDLISNSAEVGWYFLEQLKHLKEIHPIIGDVRGLGLLVGLEFVRDRK
                     TKEPFPVELGVAKRISAETFERGLLSYPGQGTADGTKGDHIMYAPPLTITKAQVDELI
                     TILDESIAVAEAEFAEDFAP"
     gene            889..1737
                     /locus_tag="VHR64_11930"
     CDS             889..1737
                     /locus_tag="VHR64_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303583.1"
                     /translation="MTPQLRLVVFSESIIADIGNPMATTVRAMCQAFADLGHDVTHLE
                     ERGNPALTRMLETRGYAPMRAFNERYPLVRYRQYELPAGTERAVWLGREVATIDAVVA
                     YPGTPLGVIDELDRRDNPLLVRFWPGAEPGVRELMPLWTEPAALTRVAPGTRDGLVVV
                     AYDNKVKADRDGWLRLSAGSATPPGWEYVPEVLIEDHYQRAQTVAMLATVDLARKLLP
                     VANGAATELIDRNGQTVEVIAGFPPVNAARPRADRLAAEITRILAERAGAAKPTSRPS
                     GDGSRV"
     gene            complement(1726..2451)
                     /locus_tag="VHR64_11935"
     CDS             complement(1726..2451)
                     /locus_tag="VHR64_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX3303584.1"
                     /translation="MRVAVMSDIHGFSLALDTVRADLEEQGPFDEIVVAGDICVVGPD
                     PQGVVERLRETNWTVVQGNTDYDLVEAAEWATGIEDLGYWVDNLTDDAMNYVAMLPFS
                     HRITPPGGTSPAHELLIVHANPHNLTDQLHPKATDRELREVIGDEQAGTIAFGHVHIA
                     YIRHVAGTMLVDVSAVGNPKDKDLRCKYGILNWDANTQRWTAEIRRLDYPLEETEAQI
                     RASDMPNPEKVIKKLKKASYSDA"
     gene            2656..5085
                     /locus_tag="VHR64_11940"
     CDS             2656..5085
                     /locus_tag="VHR64_11940"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3303585.1"
                     /translation="MPQPLTPLIGRGAELTTIRELLDRANVHLVTLTGPGGAGKTRLS
                     IEIGHLERANFGDGVVFVPLANLTSANAMLGAVAQALDVREAPGGSLADAISSVASGL
                     DVLLILDNLEQIDNPVPALTLLFDNAPEIRILATSRSALRIRGEHEVEIEPFATPDPA
                     KLPPIDELAANPAVSLFVDRASSVKPTFALGPDNAADIAEICRRLDGLPLAIELAAAR
                     SKLLSPAQMLPRLANRLQLLTSGPRDLPERQQTLRDAIAWSHDLLPEREQLLYRRMAV
                     FSGGATVDEVTIIAGFDQDNAFDDLAALVDHSLVRVIDGVDGEARYTMLQTIRDFAVE
                     LLDESGETDAIRTRMIEYYVALSDTIKAELVGAGSAHWLKVVQAEIDNLRSAHRWAVE
                     CGDAHSAQRIASALPRFWEVQGNFSEGRSWLTAALALGNEPTLDRAHALIGLATLARR
                     QGDYESAIASYEEGLAIFRSLDDRSGIATALNNLGVVAQDQGDYDRARELLTEAQQYF
                     ASIDDRPRNASALNNLGLVARRQGDMEGAVRLYEQSLALWTELGDQLRRALCLNNLGV
                     VAYYLGDNDTAENRYREALAVYRQLEDRSGAALTLNNLAEVLRDRGDYPQAMTAWQES
                     LALRSVQGDRVGIAACLIGIGRIAATSGLHDLAARLFATAVRLQTETGVSLPPRERDV
                     QDKAIAELRRSVDAATIQRSWDAGNAMPLATLLEEVANSTETLIDASTRVASKPSTTV
                     KSAAAEAGLTRREIDVLRLLVDGLSDREIGDALFISHRTAMTHVANILGKLDLESRTA
                     AAAFALRNNLV"
     gene            5412..7739
                     /locus_tag="VHR64_11945"
     CDS             5412..7739
                     /locus_tag="VHR64_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303586.1"
                     /translation="MRVLFAAVLLALVVMPLTDIGASAKDNIKIDPVILQDQPVVDPG
                     TVVDPPLVDTPTPEPTVPVLQQVPVDGPVLDDPVVLDPGSQVNDLYINPLLCPSTLDL
                     AAVDYYGLAANCNLGYADGWELIVMDSQSNWWIHNVINGGGAGQSGLPNDDYYISFTH
                     PSGGYSPHRIICKADDMLGNDVVASFDLVTDDYGGTLSVSGGLMWYCDAFIKVDGPAA
                     GPIDVAINKHGCPQGIVSTDPYFLAAMCQDMPTGINFTLTDGSSNTYTQATAGTPAMA
                     NFSQVVGGNVSIQETIPGGYQDPFVMCSVNDLLGNNLEGAYRTPAATGGLFVLDNLPA
                     DTASIFCDVFNFPTDSDGGSIIIVKRYCPVDYDIQNGDPAADCGQPQDGVQFNVSGPN
                     GYASQSNTGDSIPSAVSFGGLEAGEYQVTETLPAGVSSAWTWSCSSDWADLSGFQPED
                     HTNSPFVYDLADNEDILCFWYNVPSSDGSVTIHKWDCPEGTELGHDATWYLQYCTEPM
                     DGVEFGHGKLGDNAKLVTTDATGTVTFPVDANTDWVVEEHVPSGYGDPIVFCKWGGYK
                     KDTAGNIIAIDGITNLDGQSGAQIQFPTYDTFGMNCNWFNIPNKNWDGGDLTIYKYWC
                     TGYVVSTANCELGSGVKFVVSPNGGGSPILTQTGYDGYVTLTGLQAGSYSVTEKDYEW
                     CKATSSKVDAAGNVLIEDGQETVVTIYNCTPENGKKQPPVKTFPNTGAGSAPHGSDDE
                     IILLGSMLALAKVMVLLALRAKGLSLQMVAARIIR"
     gene            7860..8609
                     /locus_tag="VHR64_11950"
     CDS             7860..8609
                     /locus_tag="VHR64_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3303587.1"
                     /translation="MTPEIAVNLYSAREAMAQDLEGTLAAISRIGYRNVELAGYHGCD
                     PERFKRILDANNLRAVSAHVPIDRFENELEVVIGEARLLGLDALVVPWLSPEQRTVDF
                     IKDLFAKLNAWGEKVVENRMRLAYHNHDFEYSIMLRDRSMMEILLADTHADFVDLEPD
                     LYWIAEAGFDPVHQLLEMHPRVRMIHAKDRAADGSIANVGSGVLDWDGIFRAAERVMA
                     NYLIVEHDFPTDLVADLTASYQFLSGRLATT"
     gene            complement(8606..8845)
                     /locus_tag="VHR64_11955"
     CDS             complement(8606..8845)
                     /locus_tag="VHR64_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303588.1"
                     /translation="MAAIPPTDPNETPEPKPMDPTFPNFPDEPGVPDVGDPTEPARPD
                     IPGEGDPFGFPEPPGIPQIGDPIAFPGEDMVRPNA"
     gene            9050..9796
                     /locus_tag="VHR64_11960"
     CDS             9050..9796
                     /locus_tag="VHR64_11960"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX3303589.1"
                     /translation="MRVLLAGESWTMHTIHQKGFDSFTTTAYAEGHQWLSGALKAGGA
                     ELDYLPSHIANDQFPYTPEDLARYDVVMLSDIGSNTLLLPSDTFTKSIPRPNRLESIR
                     QYVENGGGFVMIGGYLTFQGIDAKAQYASTPVEDALPVTLMYGDDRVEAPQGITPTVD
                     EAEHPIVAGLPSSWPNLLGYNLLEPREQATVVASVGTDPLIVAWEYGEGRSVAFASDC
                     GPHWAPPDFVEWDGYATLWQQLVRWTAGDA"
     gene            complement(9793..10020)
                     /locus_tag="VHR64_11965"
     CDS             complement(9793..10020)
                     /locus_tag="VHR64_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303590.1"
                     /translation="MTNKLAARVGTLTRHMPPLGAPLAFDQSCPNSVIPAVRPASRNQ
                     WSRTDLAAAIGHDPAEPDDAARVWPPPRRRG"
     gene            10285..11430
                     /locus_tag="VHR64_11970"
     CDS             10285..11430
                     /locus_tag="VHR64_11970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3303591.1"
                     /translation="MDILQVLYYYTPHCSGVTIYAERLARHLTARGHRVTILASRHDT
                     SFPREQAVDGVRIVRVPSIYAVSRGVVMPGFLPAAAKLIRQHDVVHMHLPVLEAAGIA
                     ALSRAMRKRLVLTHHTDLTLNTGRFNHAAASAVFASGIAAGKLADTVVTYTHDRAQVS
                     PTVTRLKRNATVIYPPVEIDPPSEDGIAAFREKHNLGRGPVIGFAGRFAEEKGCDDLL
                     RTIPKVRECCPGATYAFAGEYRKVVGETFYERTKPLLDQHSEHVRMLGVLRGQELSDF
                     YAACDVLVLPSVNFTETFGLVQVEAMLCGTPVIASDLPGVREPIRVTGMGRITPPREP
                     DQLAEAIIDVISNRPSYVRPRDEIAATFQIDATIDAYERVYRGELVA"
     gene            11431..>11935
                     /locus_tag="VHR64_11975"
     CDS             11431..>11935
                     /locus_tag="VHR64_11975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3303592.1"
                     /translation="MAAISDAPTRSTAEARRIVRAAMPVEADLAFRRRCETVIEYLDA
                     GPDDTILDCGCGYGFYLRLLDDLTGATIVGLDPDQDRLDQCRRQLGDRSRVSYVRGDA
                     QALPFPAESFSYAVCSEVLEHLDNDRAAMAELFRVLRPGGALALTVPSADYPAAWDPL
                     NFMLERTV"
BASE COUNT         2094 a         4071 c         3695 g         2075 t
ORIGIN      
        1 caacctcgcg agcgccttgc cccaaagcgt ccacatcgcg ccatgctatc ggtatcgata
       61 tgccgacccg cgcgacgaga gttacggggt cactgccgcg atggagctcg accgcacgat
      121 ccgcaacctc ggccccgaga acgtggccgc gttcattgcc gaaccggtcg tcggcgcgac
      181 cctcggcgcg gtccccggcg agcgcagcta cttccagcgc attcgccgga tctgcgacga
      241 gcacaatgtc ctgctgattt cggacgaggt tatgaccggg ttcggtcgaa ccggtctcgc
      301 ctttggaatc gatcactggg gcgagacgcc ggacctgatc acctgcgcca agggcatcgc
      361 cggcgggtat tcgccacttg gagcggtcat cgccaggtcg gagatcgtca gcgaagtacg
      421 gcagcgctcg ggctcgttca tgatcggcgg gaccgcttcc ggcaatccgc tctccagcgc
      481 gaccgccatc gccgtcctga actacatcgt cgagcacgac ctgatctcga attcggcgga
      541 ggtcggctgg tatttccttg agcagctcaa gcacctcaaa gaaatccatc cgatcatcgg
      601 cgacgtccgt ggcctcggcc tgctggtcgg tctcgagttc gtcagggatc gcaagacgaa
      661 ggagccgttc ccggtcgagc tgggcgtggc aaagcgcatc tcggccgaga cgttcgagcg
      721 cggcctgctc tcctatcccg gtcaaggcac ggccgacggc acgaagggcg atcacatcat
      781 gtatgcgccg ccgctaacga tcacgaaggc gcaggtcgat gagctgatca cgatcctgga
      841 cgagtcgata gccgtggctg aggcggagtt cgccgaggat ttcgcgccgt gaccccgcag
      901 ctccggctgg tcgtcttcag cgagtcgatc atcgcggata tcggtaaccc gatggcgacg
      961 acggtacggg ccatgtgcca ggcattcgcc gacctcggcc atgacgtcac gcacttggaa
     1021 gagcggggaa atccggcttt gacccggatg ctcgagacgc gcggctacgc cccgatgcgc
     1081 gccttcaacg agcgctaccc gctggtccgc tacaggcaat atgagctgcc ggcgggcacc
     1141 gagcgagccg tctggctcgg gcgcgaagtg gccaccatcg acgccgtggt cgcctatccc
     1201 ggcactccct taggggtgat agatgagttg gaccggcgcg ataatccgct gctggttcgc
     1261 ttctggccag gcgcggagcc tggcgttcgc gagttgatgc cactctggac cgagccggcg
     1321 gcgctcaccc gcgtggcacc cgggacgcgc gatggtctgg tcgttgttgc gtacgacaat
     1381 aaagtcaaag cggaccggga tgggtggcta cgattgtctg ccggttcggc aaccccgccc
     1441 gggtgggagt acgtgccaga ggtgttaatc gaggatcact atcagcgtgc gcagacggtg
     1501 gcgatgctcg ccacggtgga tctcgcgcgc aaactgttgc cggtcgccaa tggggcggcc
     1561 accgagctga tcgaccgcaa tgggcagacg gtcgaggtca tcgctggctt tcccccggtc
     1621 aacgccgcca ggccccgcgc cgatcgactc gccgccgaga tcacgcgcat tcttgcggaa
     1681 cgagccggag cggcgaagcc aacatcacga ccctccggcg acgggtcacg cgtctgaata
     1741 cgaggccttt ttcagcttct tgatgacctt ctcgggattc ggcatgtcgc tggcccggat
     1801 ttgggcctcc gtctcctcaa gcgggtaatc gaggcgccgg atctcggctg tccagcgctg
     1861 ggtattcgcg tcccagttca ggatcccgta tttgcagcgc aaatctttgt ccttcggatt
     1921 gccgaccgcc gaaacatcaa ccagcatggt cccggcgacg tgccggatgt aagcgatgtg
     1981 aacgtggccg aaggcgatcg tgcccgcctg ctcgtcgccg atgacctcgc gcagctcgcg
     2041 gtcggtcgct ttcgggtgga gctggtcggt caggttatgc ggattggcgt gcacgatcag
     2101 cagctcgtgg gccgggctcg ttcctcccgg cggcgtgatc cggtgactga acggcaacat
     2161 ggcgacatag ttcatggcat cgtcggtcag attgtcgacc cagtaaccca gatcttcgat
     2221 cccggtcgcc cactccgccg cctcgaccag gtcgtagtcg gtgttgccct ggaccaccgt
     2281 ccagttcgtc tcgcggaggc gctcgacgac gccctgcgga tcgggaccaa cgacgcagat
     2341 gtcgccggcg accacgatct cgtcgaatgg accctgctct tccaggtccg cgcgcaccgt
     2401 atccagcgcc aggctgaacc catggatgtc agacatcacc gcgacgcgca tgcgctcctc
     2461 catcattgac cggactctac cagccgcggg ttagcctccc gcctcctgct gctctgatcc
     2521 tacatggtcc ggccgatttg acgaagcatg tcaggaacgg catgatcatg ccgattggtg
     2581 ccgcagaatc tggatgcgaa agacggtgct cccccgaccg tgactgcccc tactgccata
     2641 cgaccgcatt tcgccgtgcc acagccactg acgccgctga tcgggcgcgg cgccgagctc
     2701 acgacgatcc gggagttgct cgaccgcgcc aatgtccatc tggtcacctt gactggccca
     2761 ggcggggccg gcaagacccg gttgtcgatc gagatcgggc acctcgagcg ggccaacttc
     2821 ggagacggcg ttgtcttcgt cccactggcg aacctgacct ctgccaatgc catgctcgga
     2881 gccgtcgcgc aggcgctcga cgtccgcgaa gcgcccggcg ggtcgctggc cgatgcgatc
     2941 agcagcgttg catccggcct cgacgtgctc ctgatccttg acaacctcga gcagatcgac
     3001 aacccggtcc cggccctgac ccttctcttt gacaacgcgc ccgagattcg gatccttgcc
     3061 acgagccggt cggccctgcg gattcgtggc gagcacgagg tcgagatcga gccattcgcg
     3121 acgccggatc cagccaaatt gccgccgatc gatgagttgg ccgctaaccc ggcagtgtcg
     3181 ctttttgtcg accgcgccag ctcggtcaag ccgacctttg cgctcggccc cgacaatgcc
     3241 gccgacatcg ccgaaatttg tcgccggctg gacggattgc cgctcgccat cgagttggct
     3301 gccgcccgat cgaagctact cagccccgcc cagatgctgc cgcgcctggc gaaccgtctc
     3361 cagctcctga ccagcgggcc gcgcgatctc ccggagcgcc agcagaccct gcgcgacgca
     3421 atcgcctgga gccatgacct cctgcccgag cgcgagcagc ttctttaccg gcgcatggcc
     3481 gtcttctcag gcggtgcgac ggtcgacgaa gtgaccatca tcgccggctt cgaccaggac
     3541 aacgccttcg acgacctggc cgcgctggtg gatcacagcc tggtccgcgt catcgatgga
     3601 gtagacggcg aagcgcgtta cacgatgctg caaacgatcc gggattttgc cgtggagctt
     3661 ctggacgaat cgggcgaaac ggatgcgatc cgaactcgca tgatcgagta ttatgtcgcg
     3721 ctcagcgata cgatcaaggc cgagctggtc ggcgccggct ccgcgcactg gctgaaggtc
     3781 gtccaggcgg aaatcgacaa tctccgttcc gcgcatcgct gggctgtcga atgcggcgat
     3841 gcacattcgg cgcagcggat cgccagtgcg cttccccgct tttgggaggt gcagggcaac
     3901 ttcagcgagg ggcgatcgtg gttgacggcc gctctggcgc tcggcaatga gccgaccctg
     3961 gaccgagccc atgccctgat cggcctcgcc acgctggccc gccgccaagg cgattacgaa
     4021 tcggccatcg cctcatatga ggaagggctc gcgatcttcc gctcgctcga cgaccgctcg
     4081 ggcatcgcca ccgcgcttaa caacctcggc gtggtcgcgc aggatcaagg cgactacgac
     4141 cgggcgcgcg aattgctgac cgaggcgcag cagtatttcg cgtcgatcga cgaccggcca
     4201 cgcaacgcct ccgccctcaa caacctggga ctcgtcgcgc ggcggcaggg cgacatggag
     4261 ggcgcggtcc gcctttacga gcagagcctc gccctctgga ccgagctggg cgatcaactc
     4321 cggcgcgcgc tctgcctcaa caatctcggc gtcgtggcct attacctggg cgataacgac
     4381 accgccgaga accgctaccg tgaggcgctc gccgtctatc gccaactcga ggaccgcagc
     4441 ggcgccgccc tgaccctcaa taacctcgcc gaggtcctgc gcgaccgggg cgattatccg
     4501 caagcaatga ccgcctggca ggaaagcctc gccctccgtt cggtccaggg cgatcgggtc
     4561 ggcatcgccg cctgcctgat cggaatcggc cgcattgccg cgacctctgg cctgcatgac
     4621 ctcgccgctc gcctgttcgc cacggccgtc cggctgcaga ccgagaccgg cgtctcgctg
     4681 ccgccgcgcg agcgcgatgt ccaggacaag gccatcgccg agctgcggcg atcggtcgac
     4741 gccgcgacga tccagcgctc gtgggatgcc ggcaatgcca tgccgctcgc gaccctgctc
     4801 gaagaggttg caaacagcac cgagacgctg attgacgcgt ccacccgggt ggcatccaag
     4861 ccaagcacga ccgtcaagtc cgccgcggcc gaagccggcc ttactcgccg cgagatcgac
     4921 gtcctccgcc tgcttgtcga cggcctgtcc gaccgagaga tcggcgacgc cctcttcatc
     4981 agccatcgca cggccatgac ccacgttgcc aacatcctcg gcaagctcga tctcgaaagc
     5041 cgcaccgccg cagcggcatt tgcgctgagg aacaacctgg tctgacggag tctccggtct
     5101 ttggtctttg gtctctggtt ttccgtagtc cgttatccga gatccatcca ccaacaacca
     5161 accaccctca accgactgct cgcctgctcg cctcccgact gctcgcctca ggtacgtgat
     5221 ctcacgtagc cgcgaaacct catccccgcg accgcaaata cgtggccttg ccgatgttcc
     5281 ggtgctgtcc aaaaccgacg atgaaggtgt cttcggacgg ttggtttaca gcgcggtggc
     5341 gcattggaga ggacagagcg atgaggacgc agacgacgcg acagttccgg agacggcggc
     5401 aaggaggtct gatgcgggtc ctctttgcgg ccgttctgct agccctggtg gtcatgccgc
     5461 tgaccgacat cggcgccagc gccaaggaca atatcaagat cgacccggtc atcctccagg
     5521 accaaccggt cgtcgatcct ggcacagtgg tcgatccgcc gctggtggat acgccgaccc
     5581 cggagccgac cgtaccggtc ctgcaacagg tcccggtcga cggcccggtt cttgacgatc
     5641 cagtggtcct ggaccccggc agccaggtca atgacctcta catcaacccg ctcctctgcc
     5701 cgtcaaccct ggacctggca gcggtcgact actacggtct cgccgccaac tgcaacctcg
     5761 gttacgccga cggttgggag ctgatcgtga tggacagcca gtccaactgg tggatccaca
     5821 acgtcatcaa cggcggcggc gcgggccaga gcggtctgcc gaacgatgat tactacatct
     5881 cgttcacgca tccgtccggc ggctacagcc cgcaccggat catctgcaag gccgacgaca
     5941 tgctcggcaa cgatgtcgtc gcctcgttcg acctcgttac tgacgactac ggtggaacgc
     6001 tctcggtctc cggcggcctg atgtggtact gcgacgcctt catcaaggtc gatggcccgg
     6061 cggccggccc gatcgatgtc gcgatcaaca agcacggctg cccgcagggc atcgtcagca
     6121 ccgatcccta cttcctcgcc gcgatgtgcc aggacatgcc gaccggtatc aacttcaccc
     6181 tgaccgacgg ctccagcaac acctacacgc aggcgacggc cggtactccg gcgatggcga
     6241 acttctccca ggtcgtcggc ggcaacgtct cgatccagga gacgatcccg ggcggttacc
     6301 aggatccctt cgtgatgtgc agcgtcaacg acctgctcgg caacaacctc gaaggcgcgt
     6361 accggacgcc ggccgcgacg ggcggactct tcgtccttga caacctcccg gccgacaccg
     6421 cctccatctt ctgcgacgtc ttcaacttcc cgaccgactc ggacggcggc tcgatcatca
     6481 tcgtcaagcg ctactgcccg gtcgactacg acatccagaa cggcgacccg gcggccgatt
     6541 gcggtcagcc tcaggacggc gtccagttca acgtcagcgg cccgaacggc tacgcctcgc
     6601 agagcaacac cggcgactcc atccccagcg cggtctcctt cggcggcctt gaggcgggcg
     6661 agtatcaggt gactgagacc ctgccggccg gcgtctcttc cgcctggacc tggtcctgct
     6721 cgagcgactg ggccgatctg agcggcttcc agcccgaaga ccatacgaac agcccgttcg
     6781 tctacgacct ggccgacaac gaggacatcc tctgcttctg gtacaacgtc ccgtccagcg
     6841 acggctcggt cacgatccac aagtgggact gccccgaagg caccgagctc ggccacgacg
     6901 ccacctggta cctgcagtac tgcaccgagc cgatggacgg cgtcgagttc ggacatggca
     6961 agctcggcga caacgccaag ctcgtcacga ccgatgccac cggtaccgtc accttcccgg
     7021 tcgacgccaa taccgattgg gtggtcgagg agcatgtccc ctccggctac ggcgatccga
     7081 tcgtcttctg caagtggggc ggctacaaga aggacaccgc cggcaacatc atcgcgatcg
     7141 atggcatcac gaacctggac ggtcagagcg gcgcgcagat ccagttcccg acctacgaca
     7201 ccttcgggat gaactgcaac tggttcaaca tcccgaacaa gaactgggac ggcggcgacc
     7261 tgaccatcta caagtactgg tgcaccggct acgtcgtctc caccgccaac tgcgagctcg
     7321 gctccggcgt caagttcgtg gtctccccga acggcggcgg cagcccgatt ctgacccaga
     7381 ccggctacga cggctacgta acgctgaccg gtctccaggc cggctcctac tcggtcaccg
     7441 agaaggacta cgagtggtgc aaggcgacgt cgtcgaaggt cgatgccgcg ggtaacgtcc
     7501 tgatcgaaga cggccaggag acggtcgtga cgatctacaa ctgcaccccg gagaacggca
     7561 agaagcagcc gccggtcaag acgttcccga acacgggagc cggcagcgcc ccgcacggta
     7621 gcgatgacga gatcatcctc ctcggctcaa tgctcgccct cgccaaggtg atggtgctcc
     7681 tcgccctccg cgccaagggt ctctccctcc agatggttgc ggcgcgcatc attcgctaac
     7741 cgccgcatcc gctccccaca aggctcgccg gcccgggact gttcccgggc cggcgttccg
     7801 ttacccgccg atcagcgaaa atggcgccat gtgaaccgac acgtgagagg agcaatcgag
     7861 tgacgcccga aatcgcagtc aatctctact cggcccgcga agcgatggca caggatctgg
     7921 agggcacgct cgccgccatt tcccggatcg gctaccgaaa cgtcgagctt gctggatacc
     7981 acgggtgcga tccggagcgt ttcaagcgca tcctcgacgc caacaacctg cgtgccgtct
     8041 cggctcatgt cccgatcgac cgtttcgaga acgagctgga agtcgtgatc ggcgaggcac
     8101 gactccttgg cctcgacgca ctggtcgtgc cgtggctcag ccccgagcag cgcacagttg
     8161 atttcatcaa ggacttgttc gcgaagctca acgcctgggg cgagaaggtc gtcgagaacc
     8221 ggatgcgtct cgcctaccac aaccacgatt tcgagtattc gatcatgctc cgcgaccgct
     8281 cgatgatgga gatcctgctg gccgacaccc acgccgattt cgtcgacctc gagcccgatc
     8341 tctactggat cgccgaggcc ggcttcgacc cggtccacca gcttctcgag atgcacccgc
     8401 gcgtccgcat gattcacgcc aaggaccgcg ccgccgacgg ctcgatcgcc aatgtcggca
     8461 gcggcgtact cgactgggac ggcattttcc gggcggcgga gcgcgtgatg gccaattacc
     8521 tgatcgtgga gcacgacttt ccgacggacc tggtcgccga cctcaccgcc agctatcagt
     8581 tcctgtcagg tcgcctcgca acaacctagg cgttcggacg gaccatgtcc tcacccggga
     8641 atgcgatcgg gtcgccgatc tgcgggatgc cgggcggctc cgggaagccg aatgggtctc
     8701 cctcacccgg gatgtctggc ctggccggtt cggtcggatc gccgacgtcc ggcacacccg
     8761 gctcatcggg aaagttcggg aaggtcggat ccatcggctt tggctccggc gtttcgttgg
     8821 gatcggttgg gggaattgcg gccattggcg tcgctccttt gcaagtgagt ggtgagggct
     8881 ggcgaggcaa gtctaggaac ggctccgtca tccgctgtag tccgcgcggc ccgagccagg
     8941 aaccgggact acaagccggg gtgttcgagg cgcacgtttc gataaacgcg gatactaccg
     9001 gctggaattc gccctcctgg tcggaggctc gcaacaacga ggggtaacca tgcgcgtact
     9061 gctggccggt gaatcgtgga cgatgcacac gatccaccag aagggcttcg attcgttcac
     9121 cacgacggcc tacgccgagg gccaccagtg gctgtccggc gcgctcaagg ccggcggcgc
     9181 cgagctcgac tacctgccga gccacatcgc caacgaccag ttcccgtaca ccccggagga
     9241 tctggccagg tacgatgtcg ttatgctcag cgacatcggc tcgaacacgc tgctgctgcc
     9301 ttcggacacc ttcaccaagt cgatcccccg gccgaaccgg ctggagtcga tccggcaata
     9361 cgtcgagaac ggcggcggct tcgtgatgat cggcggctac ctgaccttcc agggcatcga
     9421 tgccaaagcc cagtacgcca gtacgccggt cgaggatgcg ctcccggtca cgctcatgta
     9481 cggcgacgac cgggtcgagg cgccacaggg catcactccg acggtcgatg aggccgagca
     9541 cccgatcgtt gccgggcttc catcgagctg gccgaatctg cttgggtaca acctgctcga
     9601 gccacgcgag caagcgacgg tcgttgcgtc ggtgggcacc gatccgttga tcgtcgcctg
     9661 ggagtacggc gaaggccgct cggttgcctt cgccagcgat tgcggaccgc attgggctcc
     9721 gcccgacttc gtcgaatggg acggctacgc cacgctctgg cagcagctcg tccgctggac
     9781 cgccggcgac gcctaaccgc gccgacgcgg cggcggccag actcgagcag catcatccgg
     9841 ctcggccgga tcgtgaccga tcgctgcggc caggtcggtc cgcgaccatt ggttgcggct
     9901 ggccgggcgg acagccggga tcaccgagtt cgggcagctc tggtcgaagg caagcggggc
     9961 gccgagcggc ggcatatgac gcgtcagcgt gccgacacgg gcagcgagtt tattggtcat
    10021 tccgtggaag cgatacatcg ttcgccctcc ggtggtcatc attccggtgc acatcagtgg
    10081 gcgttcggta ggaggcagcg gcgacctccc catacatctc gacggtatca cgtcgtcatc
    10141 accccgtcat gaacaaaacg ttacaaaccg gcctgacgtt cttggtcatt ccgtccaatg
    10201 gtgaagccgt gcgcaaacgc cggaacgtgc gacaatggcg tcctgacgcc atgttcaccg
    10261 tttctcggcc aggggtttcc gctcgtggat atcctccagg tgctctacta ctacacgccg
    10321 cactgcagtg gcgtgacgat ctacgccgag cgcctggcgc ggcacctgac tgcgcgcggt
    10381 catcgcgtca ccatccttgc ctcccgtcac gacacctcat ttccccgcga acaggccgtt
    10441 gatggggtcc ggatcgtgcg ggtgccgagc atctacgccg tcagtcgcgg agtcgtcatg
    10501 cccggcttcc tgccggccgc cgcgaagctc atccggcagc acgacgtggt ccacatgcac
    10561 ctgcccgtcc tggaggcggc cggaattgcg gcactcagcc gggccatgcg caagcggctc
    10621 gtgctgaccc accacaccga cctgacgctc aacaccggac ggttcaacca cgccgcggcg
    10681 agcgcggtgt tcgcgagcgg gattgccgca ggcaagctgg ccgacacggt cgtcacctac
    10741 acgcacgatc gcgcgcaggt ttcgccgacc gtaacccggc tgaagcgaaa tgcgaccgtg
    10801 atctacccgc cggtcgagat cgatccaccg tccgaagacg gcattgccgc gtttcgcgag
    10861 aaacacaatc tgggtcgggg cccggtcatc ggatttgccg gacgcttcgc cgaggagaag
    10921 ggctgcgacg atctgctacg gaccattccc aaggtccggg aatgctgccc gggcgcgaca
    10981 tacgccttcg ccggggagta tcgcaaggtc gtcggcgaga ccttctatga acggaccaag
    11041 ccactgctcg accagcacag cgagcacgtc cggatgctcg gcgtcttgcg cgggcaggag
    11101 ttgtccgact tctatgctgc gtgcgatgtg ctggtgctgc cgagcgtcaa cttcaccgag
    11161 acgttcggcc tggttcaggt cgaggcgatg ctctgcggca cccccgtcat cgccagcgac
    11221 ctcccgggcg tgcgcgagcc gatccgtgtc acgggaatgg gtcggatcac gccgccgcgc
    11281 gagccggatc agttggctga ggcgatcatc gacgtgatct cgaaccggcc gtcgtatgtc
    11341 cggccgcgcg acgagatcgc cgcgaccttc cagattgatg ccacgatcga cgcctatgag
    11401 cgggtctacc gcggcgagct ggtcgcctga atggctgcga tctccgacgc accgaccagg
    11461 agtacggccg aggcgcgacg gatcgtgcgc gcggcgatgc cggtcgaggc ggacctcgca
    11521 ttccggcggc ggtgcgagac cgtgatcgag tacctcgatg ccggaccgga cgacacgatc
    11581 ctcgattgcg gctgcggcta tggcttctac ctccgcctgc tcgacgacct aaccggggcg
    11641 acgatcgttg gcctcgaccc cgatcaggac aggctcgatc aatgtcgccg ccaactcggc
    11701 gaccgatcgc gggtctcgta tgtccggggc gacgcgcagg cgctgccgtt ccctgctgaa
    11761 tcgttcagct acgccgtctg ttcggaagtc ctcgagcatc ttgacaacga ccgcgcggcg
    11821 atggccgagc tcttccgcgt cctccggccc ggcggagcgc tggccctgac cgtgccatcg
    11881 gccgactacc ccgctgcctg ggacccgctc aacttcatgc tcgagcggac agtac
//
LOCUS       DATAOZ010000470         6397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2297110, whole genome shotgun sequence.
ACCESSION   DATAOZ010000470 DATAOZ010000000
VERSION     DATAOZ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297110"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VHR64_11980"
     CDS             <1..997
                     /locus_tag="VHR64_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX3303593.1"
                     /translation="AMFAAAESVHGELHRYLQTKARLLGIEQMAHYDLSAPISTDQKE
                     VTWPEAEGTVRSVLGPFSERLASVVDAALTQRWVDAEPREGKVGGGYCASLQPYGSRI
                     MMNFVPSFDTTQTLAHELGHAHHNACLGERSSLQKETPMTLAETASMFCQRMVEEKVL
                     AEASPEDKLIALDQILSNSVGTLLDLHARFHFEQELFARRETRTLAVDELTRLMAEKQ
                     QTAYGPGLAADGVHKWQWVQKPHYYDTDFHYYNYPYQFGLLFALGLLKVYRDLPDGFA
                     ERYETLLSRTGMATAYDLAKEFGIDIRDRAFWDGSVAVIRGDVAQFERVAAQSTR"
     gene            complement(982..1647)
                     /locus_tag="VHR64_11985"
     CDS             complement(982..1647)
                     /locus_tag="VHR64_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX3303594.1"
                     /translation="MIDPSYPHVQEHVETLPIKRSRDTGSEIRRWILTLLITVILALG
                     LLGGSLVVAIYMAARSDQEQRADAIVVMGAAQFNGRPSAVLKARLDMTYQVWKDGVAP
                     VIIVTGGKMPGDRFTESEASRDYLVDLGVPENAILLENEGRTSQASLRRVGTIARDHD
                     IHSVVIVSDGFHLFRSKLIADRNGLDAFGVAARDSPIRPWTTTEFEYVVREAGGVLAY
                     LVL"
     gene            complement(1644..2777)
                     /locus_tag="VHR64_11990"
     CDS             complement(1644..2777)
                     /locus_tag="VHR64_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEX3303595.1"
                     /translation="MDREHLHGVRMGEQEAEISEAKALERRQENLEHGLESAWSIRDR
                     TIPLFRRDGYGFSPKGSFMGGPSLTDMRELGGQDVAVVGVPLDTGTTFRSGTRFGPMA
                     MRQISAVNKGFSFEMGVDLSESLDIVDVGDVSVIPANIEKSFDQIDKAVAYIHERDVF
                     PVILGGDHSIGYPDIRGLAPYVDGNVGIIHFDRHADMSEYNMDERMHGTPFFHATNIE
                     NAPPSNLVQIGIGGWINSRPSVRNARDKGATSITLTDIDRWGLERIAEMALEIAWKNA
                     KAVFLSFDIDSMDPAFAPGTGTPEAGGLLPREAFRLLHQVAREGLAGMEVVEVSPPYD
                     VADVTSLLGVRVILETLGTLVLNGKLGKRPQPAPTTAAGEPVS"
     gene            complement(2777..4783)
                     /locus_tag="VHR64_11995"
     CDS             complement(2777..4783)
                     /locus_tag="VHR64_11995"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX3303596.1"
                     /translation="MRRIAGDVQYMTLEIRPNSDLAWTPTQAYRDRSRLLALMTQHGI
                     ESVDEWRAWAADDIGRYWGTVVEDLGLEFSAPYSAVVDVSKGAPWAEWFVDGGFNYVA
                     NALDRHAAGPERDRAALIWEGDDGTQRIVTFADLLAETNRVSNVLRNLGVERGDVVGI
                     FMPMAPETVAAVLACGKIGAVYTPLFSGFGAEAVASRLRDCDAKVLITADGFLRRGNV
                     VPMKPVADEAVAASPTVEQVLVLRRAGNDVAMTENRDIWWHDVVPVASPDCETVETRA
                     NEPYMVIYTSGTTGRPKGAVHVHAGFPIKAAHDLAYCFDLHDDDVLFWYTDLGWMMGP
                     WAIGGGLMLGATLMLFEGTPDYPNPDRIWEIVERHTVTILGIAPTAIRALIPRGDDWP
                     ARHDLSSLRVLGSTGETWNPEPWRWYFERIGGGQLPIVNYSGGTETGGGIVGCDTISP
                     IKPCCFTGPVVGMDADVVDDVGNPVRGAVGELVIRQPWVGMTRGFWRDPERYIDTYWS
                     RMPGLWVHGDWAEIDEDGFWFIRGRSDDTLKVAGKRVGPAEVESAAVAHDNVQEAAAI
                     GIPHPVKGEAVVVLCILKGHRDASGELAEEIRQTVGHQLGSALRPECVHFVRELPKTR
                     NAKIMRRVIKAAYLGLPPGDITALENPAAVDQVAAIGRSARGEA"
     gene            4873..5739
                     /locus_tag="VHR64_12000"
     CDS             4873..5739
                     /locus_tag="VHR64_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303597.1"
                     /translation="MDRLKFWRRSQPEQPAIEPIPTIKRFNIQPRTDVGTLGLPTDPE
                     KATRLVALRKRREALAHDVESATQAATDDNRWRAEIKLIVQAIHEIDADLAAINAAQP
                     DPPGTPLPATPITDVSVDVGPVARVRFRIGDTLFSYAEEIDWAERGTQLARSELIRDS
                     GSAEEIIPASVPADQRPALLEHLGQSLFAFASDLRDRTLNREPLPNATLADLARPSTE
                     YGGWLDWLGQSAGKQEREVTKSRLRFERERLMADRARLIEDQNKVAENLPIARRRLAE
                     LDREIDAITAGT"
     gene            complement(5723..6055)
                     /locus_tag="VHR64_12005"
     CDS             complement(5723..6055)
                     /locus_tag="VHR64_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303598.1"
                     /translation="MEIGARSEWRDWLASPVPNCDDIWVVIFKKASGKQSVSFDELLE
                     EALCWGWVDTQTRGVDEERYCIRFRRRRPGSNWSATNRRIICRLLAEGRVQAAGQATL
                     PPDLTCPP"
     gene            complement(6074..>6397)
                     /locus_tag="VHR64_12010"
     CDS             complement(6074..>6397)
                     /locus_tag="VHR64_12010"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX3303599.1"
                     /translation="QCVGVAVKEGLSTTSALHLHPLKAQELIREGARQAVANAKTAKP
                     YMLPKDVTVEIDFEHQSRADYAARVPRVERVGERTISYKPCDGVELITIFRAISSAAS
                     IRFSP"
BASE COUNT         1160 a         2139 c         1991 g         1107 t
ORIGIN      
        1 cgcgatgttc gctgccgcgg agtcggtcca tggtgagctg caccgctacc tacagaccaa
       61 ggcccgactg ctcggaatcg agcagatggc gcactacgac ttgtcagctc cgatcagcac
      121 agaccagaag gaagtgacct ggcccgaagc cgaaggcacc gtccggtccg tcctcggccc
      181 cttctcggag cggctggcgt cggtggtcga tgccgcgctg acccagcggt gggtcgatgc
      241 tgagccgcgc gagggcaagg ttggcggcgg ctactgcgcc tcgctgcagc cctacggctc
      301 gcggatcatg atgaacttcg tgccatcgtt cgacacgacc cagaccctcg cccacgagct
      361 cggtcacgca catcacaatg cctgcctcgg cgagcgctca agcctccaga aggagacgcc
      421 gatgacgctg gccgagaccg cgagcatgtt ctgccagcgg atggtcgagg agaaggtgct
      481 ggccgaagcc tcaccagagg acaaactgat cgcgctggat cagattctct cgaacagtgt
      541 cggcaccctg ctggacctcc atgcccgttt ccacttcgag caggaactct tcgcccggcg
      601 tgaaacccgg acactggccg tcgacgagct gacgagattg atggccgaga agcagcagac
      661 cgcatatggt ccgggcctgg ccgccgacgg tgtccacaag tggcagtggg tccagaagcc
      721 gcactactac gacaccgatt tccactacta caactacccg taccagttcg gactgctctt
      781 cgcgctggga ctgctcaagg tctaccggga tctgccggac ggcttcgccg agcgttatga
      841 gacgctgctt tcgcgcactg gcatggcaac cgcctacgac ctcgcgaagg agttcgggat
      901 cgacattcgt gaccgcgcct tctgggacgg gagtgtggcc gtgatccggg gtgatgtcgc
      961 ccagttcgaa cgggtcgccg ctcagagcac caggtaagcc aggactccac ccgcctcccg
     1021 gacgacatac tcgaattcgg tcgtcgtcca ggggcgaatc gggctgtctc gcgccgccac
     1081 gccgaacgca tcgagcccgt tgcggtcggc gatcaacttg ctgcgaaaga ggtgaaaccc
     1141 gtcggagacg atcacgacgg agtggatgtc gtggtcccgg gcgatcgtgc cgactcgtcg
     1201 gaggctcgcc tgggaggttc tcccctcatt ttcgagcagg atcgcattct ccggaacgcc
     1261 gagatcgacc aggtaatccc ggctcgcctc ggactcggtg aagcggtcgc cgggcatctt
     1321 gccgccggtc acgatgatca ccggcgcgac gccgtccttc cagacctggt aggtcatgtc
     1381 gagtcgggcc ttgaggaccg cgctcggtcg cccgttgaac tgggccgcgc ccatcacgac
     1441 gatggcgtcg gcccgctgct cctgatcgga gcgagcggcc atgtagatcg ccacgaccaa
     1501 tgatccacca agcaagccga gtgccagaat cacggtgatg aggagcgtca ggatccaacg
     1561 ccgaatctca ctcccggtat cgcgggagcg tttgatcggt agagtctcga catgttcctg
     1621 aacatgcgga tatgaggggt cgatcacgaa acgggttcac ctgccgctgt ggttggcgcg
     1681 ggttgcggcc gcttgccgag cttgccattc agcaccagcg tgccgagcgt ttcgaggata
     1741 acccgtaccc cgagcagcga ggtgacatca gcaacgtcgt acggaggcga gacttcgacg
     1801 acctccatcc cggccagccc ctcgcgcgcg acctggtgca gcaggcgaaa tgcctcgcgg
     1861 ggcagcaatc cgccagcttc cggcgtgccc gtgccaggtg cgaaggcggg gtccatcgaa
     1921 tcgatgtcga acgacaggaa gacggccttc gcattcttcc aggcgatctc gagagccatc
     1981 tcggcaatcc gctccagacc ccagcgatcg atgtcggtca gggtaatcga ggtcgcgccc
     2041 ttgtcccggg cgttccggac gctcggccgc gagttgatcc agccgccgat accgatctgg
     2101 accagattcg agggcggcgc attctcgatg ttcgtggcat ggaaaaacgg cgtgccgtgc
     2161 attcgctcgt ccatgttgta ctcagacatg tcggcgtgcc gatcgaaatg gatgatgccg
     2221 acgttgccgt cgacgtacgg cgccagcccg cgaatgtccg ggtagccgat cgagtggtcg
     2281 ccgccgagaa ttaccgggaa gacgtcgcgc tcgtggatgt aggcgacggc cttgtcgatc
     2341 tggtcgaagc tcttctcgat attggccggg atgaccgaga cgtcgccgac atcgacgatg
     2401 tcgagcgact cggagaggtc gacccccatc tcgaagctga agcccttgtt gacggcggag
     2461 atctgccgca tcgccatcgg tccgaatcgg gtcccggagc ggaacgtcgt accggtatcg
     2521 agcggcacgc caaccaccgc gacatcctga ccaccgagct cccgcatgtc ggtcagcgat
     2581 ggaccaccca tgaaactgcc ctttggcgaa aagccgtagc cgtcgcgccg gaagagcggg
     2641 atcgtccggt cccgtatgct ccaagccgac tccagcccgt gctcgaggtt ctcctggcgg
     2701 cgctcgagcg cctttgcctc ggaaatctct gcttcctgct cgcccatcct gacgccgtga
     2761 aggtgctcgc gatccattac gcctctcccc gggccgagcg gccaattgca gctacctgat
     2821 cgaccgcggc cggattctcg agcgcggtga tgtctcccgg cggaagtcca aggtaggctg
     2881 ccttaatcac ccggcgcatg atcttggcgt tccgggtctt gggcagctcc cgcacgaaat
     2941 ggacgcactc cggccggagc gcggaaccga gctgatgacc gaccgtctgc cggatttcct
     3001 ctgccagctc gccggatgcg tcgcggtggc ctttcagtat gcaaagcacg accactgcct
     3061 cgcccttgac ggggtgcgga atgccgatcg ccgccgcctc ctggacgttg tcgtgcgcga
     3121 cagcggccga ttcgacttcg gcaggaccga cgcgcttgcc cgcgaccttg agcgtatcgt
     3181 ccgagcggcc ccggatgaac cagaagccat cctcgtcgat ctcggcccag tcgccatgca
     3241 cccacaatcc gggcatccgt gaccagtagg tgtcgatgta ccgctcaggg tcgcgccaga
     3301 atcctcgcgt catcccgacc cacggctgcc ggatcaccaa ctcgccgacc gcgccgcgca
     3361 ccgggttacc cacgtcgtcg actacatcgg catccatccc gacgaccggc ccggtgaagc
     3421 agcatggctt gatcggcgaa atggtgtcgc agccgacgat gccaccaccc gtctcggtgc
     3481 cgcccgagta gttgacgatc gggagctgtc cgccgccgat ccgctcgaag taccagcgcc
     3541 agggttcggg gttccaggtc tcgccggtcg agccgagcac gcgcaacgac gacaagtcgt
     3601 gccgcgccgg ccaatcgtcg cccctgggaa tcaatgcgcg gattgcggtc ggcgcgatcc
     3661 caaggatcgt caccgtgtgc cgctcgacga tctcccaaat ccggtccggg ttcgggtaat
     3721 ccggcgtccc ttcgaagagc atcagggtgg cgccgagcat caggccgccg ccgatcgccc
     3781 acggtcccat catccaccca agatcggtgt accaaaagag cacgtcgtca tcgtgcaggt
     3841 caaagcaata agccagatcg tgcgcggcct tgatcggaaa cccggcatgg acgtggaccg
     3901 cgcccttcgg ccggcccgtc gtgcccgacg tgtagatgac catgtacggc tcgttcgcgc
     3961 gcgtctcgac cgtctcgcag tcaggagatg cgaccgggac gacgtcatgc caccagatgt
     4021 cgcgattctc ggtcatcgcg acgtcgttgc cggcgcggcg caggacgagt acctgctcga
     4081 ccgtcggcga tgcggcgacc gcctcgtcgg cgaccggctt catcggcacg acattgccgc
     4141 gccgcaggaa gccgtcggca gtgatcagca ctttcgcgtc gcagtcgcgc agccgggaag
     4201 ccaccgcctc ggcgccaaaa ccggagaaga gcggcgtata aaccgcgccg atcttgccac
     4261 aggccagcac ggccgccacg gtctccggcg ccattggcat gaagatgccg acgacatcgc
     4321 cacgctccac gccaaggttg cgcagcacgt tggacacgcg attggtctcg gccagcaaat
     4381 ccgcgaaggt gacgatgcgc tgggtgccgt cgtccccttc ccagatcagc gccgcccggt
     4441 cgcgctccgg cccggcggca tggcgatcga gcgcgttcgc aacgtagttg aagccgccgt
     4501 cgacgaacca ctcggcccag ggcgcgccct tcgacacgtc aaccactgcc gaatacggcg
     4561 ccgagaattc gaggccaaga tcctcgacga ccgtacccca gtagcggccg atatcatcgg
     4621 ctgcccatgc tcgccactca tcgaccgatt caatgccgtg ttgtgtcatg agagcaagga
     4681 gtcggctgcg atcgcggtag gcttgagtcg gtgtccaggc gaggtcggaa ttcggtcgga
     4741 tttcgagcgt catgtactgg acatctcccg cgatgcgccg catggtagtc gtgcctgcaa
     4801 tactgaccgg gtccgggaat gcggtcaagg tcggtctttc tggacacata tcatcttctg
     4861 aggagtcacg ctgtggatcg tctgaaattc tggaggcgca gtcaacccga acagccggcc
     4921 atcgaaccca ttcccacgat caagcgcttc aacattcagc cccggaccga cgttggcacg
     4981 cttggcctgc ccaccgatcc ggagaaggcg accagactgg ttgccctgcg caagcgccgg
     5041 gaggcactgg cccacgatgt ggaatcagca acccaggcgg ccaccgacga caatcgctgg
     5101 cgcgccgaga tcaaattgat cgtccaggcg atccatgaaa tcgacgccga tctggctgcg
     5161 atcaatgccg cccagcctga ccctcccgga actccccttc ccgcgacccc gatcaccgac
     5221 gtctcagtcg atgtcggtcc ggtcgcccga gtccgatttc ggatcggcga cacgctcttc
     5281 tcgtacgcag aggagatcga ttgggctgag cgtggtaccc agctggcccg aagtgagctg
     5341 atccgcgaca gcggcagcgc ggaggagatc atcccagcgt ctgtcccggc tgatcagcgg
     5401 cccgcgctcc tggagcatct gggccagagt ctcttcgcct ttgcctcgga cctgcgagac
     5461 cggacgctga accgcgagcc tcttcccaat gcaaccctgg ccgacctcgc gcggccgagc
     5521 accgaatacg gcggttggct ggactggttg ggccaatccg ccgggaagca ggagcgcgag
     5581 gtgacaaaat cgcggcttcg attcgagcgg gagcgcctta tggccgatcg cgctcgcttg
     5641 atcgaggatc agaacaaggt cgccgagaac ttgccgatcg cgcggcgccg gctggccgag
     5701 ctcgaccgcg aaatcgacgc gattacggcg ggcacgtgag atccggcggc aaggttgcct
     5761 gtcctgccgc ctgaaccctg ccttccgcca gcagtcggca gatgatccga cggttggtcg
     5821 ccgaccaatt cgatcccggg cggcgcctcc ggaaccggat gcagtaccgc tcctcatcga
     5881 cgcctctggt ctgggtatcg acccatcccc agcagagcgc ctcctcgagc agctcgtcga
     5941 aggagacgga ctgcttgcct gacgccttct tgaagatgac cacccagatg tcgtcgcaat
     6001 tgggaacggg actggcaagc cagtcccgcc actccgagcg tgctccgatc tccatcgggt
     6061 ccacggtctg caattacggc gaaaaacgga tcgacgcggc gctgctgatt gcccggaaga
     6121 tcgtgatcag ctcgacaccg tcacatggct tgtacgaaat ggtccgctcg ccgacccgct
     6181 cgacgcgcgg aacgcgtgcg gcatagtccg cccgggactg gtgctcgaag tcgatctcga
     6241 ccgtcacgtc tttgggcagc atgtacggct tggccgtctt cgcatttgcg accgcctgcc
     6301 gggcaccctc gcgaatcagc tcctgggcct tgagcggatg aaggtgcagc gcggaggtcg
     6361 tgctcagacc ttccttgacc gcgacgccta cgcactg
//
LOCUS       DATAOZ010000471         5177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_977390, whole genome shotgun sequence.
ACCESSION   DATAOZ010000471 DATAOZ010000000
VERSION     DATAOZ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977390"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="VHR64_12015"
     CDS             complement(<1..1833)
                     /locus_tag="VHR64_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3303600.1"
                     /translation="MVLSSYAGAAVRRKEDPRLITGASTYVDDIALPNLVHAAFVRSP
                     YAHAKINGVDASEALAMPGVIAVYTESDIRTVMPDIYPKLAGDTGQDAEEIAEEDDAS
                     IPVPDVYPLAWDKVRYVGEPVAVVIAETKAQAEDAASLVVIDAEPLDAVIDPYEATED
                     GAPLIYDNVKNNISVREATVHGDVDAALNSAAIRIKANIRSPRCAPLAMEGRAIVAQP
                     DAILGGLTFWTSTQAPHWNRNDIAKALGLSQSKVRAIAPEVGGGFGSKIGTYPEEFVI
                     CAAAVLLKRPVKWIESRSENLLATNHGRNQWSEFEAGADANGKITALKARVLLDSGAY
                     PKALDLAWATWIMATGPYDIPNLDYEVLGAYTNTMANGAYRGAGRPEAAYYLERVVDL
                     IADEGGLDPVEVRRVNFIKPEQFPFTTLTGEHYDTGEHEKALNKALEVADYEGLRKEQ
                     AEALKQGRHLGIGVASYVEICGFGPYDSTKVRVEPSGDVSIFTGISPHGQGQETTFAQ
                     MAQQHIGADFERTVVHHGDTSTNPQGNGTMGSRGLPVGGAALMMSLNRIKDKATRIAA
                     HMLEAAVEDIEVVDGKYQVKGVPDRGVGLGDIAEKAYSGDLPDEI"
     gene            complement(1956..3299)
                     /locus_tag="VHR64_12020"
     CDS             complement(1956..3299)
                     /locus_tag="VHR64_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3303601.1"
                     /translation="MISVLIVADEQWAGPALSRRIASDPSIEVAGTVSSLAGALDRAD
                     ARVVDVLLVHAEFWLGAETSSLARYRSRTTALPVIIVSQTEPLFGLDALLSTGVSFVC
                     PLSVEDEILCEIIKRYAAAPNNLAPHLKNPTIIPETIRRQFAAGRWPRPPYFRPPNFP
                     NVLTETEACVLRMLGRGLSTALIGEVLGIPERAVEDQISTIRRKVGVDEHADLRHLGM
                     MGSAGRDGNPIFQVAIGDRNKTRRDAIELRLAGIADIRVLSSEGSVSAVTSNQIDVII
                     LGTDLPDAIDLNHVRGLRWPFDFVPVLAIVDQVTNDLLDIMQRGGVAELVDRNIESEA
                     LGRSIRRVARGEDPIFDRPGAASWGRPVSPTEAERVILRGFRDGAPDSEIRRALRVGR
                     WTYWQMRETMVYRLALKDRANAVRVAIRFGWIEPVSPPDDAVSSGSGSAGSLPLA"
     gene            complement(3457..4203)
                     /locus_tag="VHR64_12025"
     CDS             complement(3457..4203)
                     /locus_tag="VHR64_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3303602.1"
                     /translation="MSDTIRVLIVEREPLFRRGLAGCLATDNELQLVGSVGTAEEGYR
                     TADEQLPDVTLIGTTLADGPGLNAATEFRRRFPSIAVIVVAAQESDDELFGAIRAGAS
                     AYCGQDIDETKLITLIKRSATGEYVINEQLLDKPYVAARVLEQFRSATSSDLAPHNAF
                     APLTDRELEILRKVSDGMTNAEIGYALGISAQTVKNHVTSILRKLAVNDRTQAVVTAL
                     KRGWLSIDDSNPTGRNGVSVGSGSGMDQVR"
     gene            complement(4511..>5177)
                     /locus_tag="VHR64_12030"
     CDS             complement(4511..>5177)
                     /locus_tag="VHR64_12030"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HEX3303603.1"
                     /translation="ELLNRETEAEEQRIQSVLWGIREVLHRFGYMRRGYATEKADMLA
                     GVFDTDGLILCELIDRGLLNDLAPEDLAEVFSWYSFDRDFRYGNQFVLPPHFTPIRRR
                     IEEIERALISEEREHSLFISEGHNPSFYGPARAWCLGKTMVDVTETIQLSEGDLVLTF
                     NKTIDLVKQVREMLEDVKPEHGLIGPLRQAENLLCRGIVEQSLSIGFAPIESEPEPSV
                     TAP"
BASE COUNT          917 a         1631 c         1625 g         1004 t
ORIGIN      
        1 gatctcatcc ggtagatcgc cgctgtaggc cttctcggcg atatcgccga ggccgacgcc
       61 gcgatccggc acgcccttga cctggtactt gccgtcgacc acttcgatgt cctcgaccgc
      121 cgcctcgagc atgtgggcgg cgattcgggt cgccttgtcc ttgatccggt tgagcgacat
      181 cattagcgcc gcgccaccga ccgggaggcc gcggctgccc atggtgccgt tgccctgcgg
      241 attcgtactg gtatccccat ggtggacgac cgtgcgctcg aagtcggcac cgatgtgctg
      301 ctgggccatc tgggcgaagg tcgtctcctg gccctggccg tgcggcgaga tgccggtaaa
      361 gatcgagaca tcgccgctcg gctcgacgcg taccttggtg ctgtcgtacg ggccgaagcc
      421 acagatctca acgtatgacg caacaccgat gcccaggtgg cggccttgct tgagcgcctc
      481 agcctgctcc ttgcgcaggc cctcgtagtc ggcgacctcg agtgccttat tgagcgcctt
      541 ttcgtgctcg ccggtgtcgt aatgctcgcc ggtcagcgtc gtgaatggga actgctccgg
      601 cttgatgaaa ttgacccgac ggacctcaac cgggtcgagc ccaccctcgt cggcgatcag
      661 gtccacaact cgctcgaggt agtaggcggc ttccgggcga ccggcgccgc gataggcgcc
      721 gttggccatg gtgttcgtgt aggcgcccag cacctcgtag tcgaggtttg gaatgtcgta
      781 cgggccggtc gccatgatcc aggttgccca ggccaggtcg agcgccttgg gataagcgcc
      841 ggagtcgagc aggacgcgcg ccttgagggc cgtaatcttg ccgttcgcgt cggcgccggc
      901 ttcgaactcg gaccactggt tgcgcccatg gttggtggcg agcagattct ctgaccggct
      961 ctcgatccac ttcaccggcc gcttcagcag cacggccgcc gcgcagatca cgaactcctc
     1021 ggggtaggtg ccgatcttgg agccgaagcc tccgccgact tccggtgcga tcgcgcggac
     1081 cttgctctgg gagagaccga gcgctttggc gatgtcattc cggttccagt gcggcgcctg
     1141 cgtcgaggtc cagaaggtca gtccgccgag gatcgcatcc ggctgggcga cgatcgcgcg
     1201 cccttccatg gcgagcggcg cgcagcgggg cgagcgaatg ttggccttga ttcggatcgc
     1261 agcgctattg agggcggcat cgacgtcacc gtggaccgtc gcctcgcgca cgctgatgtt
     1321 gttcttgacg ttgtcgtaaa tcagcggcgc gccgtcttcg gtcgcctcgt acgggtcgat
     1381 caccgcgtcg agtggctcgg catcgatcac gaccaacgag gcggcatctt cggcctgtgc
     1441 cttggtctcc gcgatcacga ctgcgaccgg ctcgcctacg tagcggacct tgtcccatgc
     1501 gagcggatag acatccggca cgggaatcga ggcgtcgtct tcttccgcga tctcttccgc
     1561 atcctggccg gtatctcccg ccagtttggg atagatgtcg ggcatcacgg tgcggatgtc
     1621 ggactcggtg tagacggcga tcacgcccgg catggccagc gcctcggagg cgtcgacacc
     1681 gttgatcttc gcgtgggcgt aggggcttcg gacaaacgcg gcatgcacca gattcggcaa
     1741 cgcgatgtcg tcgacatacg tcgacgcgcc ggtaatcagc cgcgggtctt ccttgcggcg
     1801 gacggccgct ccagcgtaac tgcttagaac catggcgtgt tgctcgctcc tcttctatgg
     1861 aaaaagcgcc ggcgctggcg catccctcac tgagtctgtg gcgggattgt atcagtgtgc
     1921 gttagcccga cggtgggcag tcaagcgcga accgcttacg cgagcgggag gctaccggcc
     1981 gatcctgatc cggaactcac cgcgtcgtcc ggtggcgaaa ccggttcgat ccagccgaac
     2041 ctgatcgcga cgcgcaccgc gttcgctcga tccttgagtg ccagcctgta gaccatcgtc
     2101 tccctcattt gccaataggt ccacctcccg acacggaggg cccgccgaat ctctgaatct
     2161 ggagctccat cccgaaatcc gcgcaagatc acgcgctcgg cttcggtcgg gctcacgggc
     2221 cgaccccagg aagctgcgcc cgggcggtca aagatcggat cctcgccacg cgccacacgg
     2281 cgtattgatc ggccgagcgc ttcgctctcg atattgcggt ccacgagctc agccacccca
     2341 ccccgctgca tgatgtcgag cagatcgttc gtcacctgat cgacaatggc aaggacagga
     2401 acaaaatcaa atggccaacg aagtccgcgg acgtgattga ggtcaattgc gtcaggtaaa
     2461 tccgttccaa ggataattac atcaatttgg ttcgaagtga cggccgaaac tgagccctcc
     2521 gaactcaata cccggatgtc ggcgatacca gccaaccgaa gttcgattgc atcgcgcctg
     2581 gtcttgtttc gatcgccaat agcgacctga aaaatcgggt tcccgtctcg ccccgcgctg
     2641 cccatcatgc cgaggtgacg gagatcggca tgttcatcaa cgccgacctt gcgccggatt
     2701 gtggatatct ggtcttcgac tgcccgctcc ggaattccaa gcacctctcc aatcaaggca
     2761 gtcgatagcc ctcggcccaa catgcgcaag acgcaggctt cagtctctgt gagaacgttc
     2821 gggaaattcg ggggccggaa gtagggcggc ctcggccaac ggccagccgc aaactgccgc
     2881 cggattgttt ccgggatgat cgttgggttc ttcaaatgag gtgcaaggtt gtttggcgcg
     2941 gctgcgtacc gcttgatgat ttcgcaaaga atctcgtcct cgacgctcaa cgggcacacg
     3001 aaactgacac cggtgctcag cagggcgtcg agaccgaata ggggttcagt ctggctgacg
     3061 atgatcaccg gtagcgcggt cgtgcgggat cgatacctgg cgaggctgga tgtttcagcg
     3121 ccgagccaga attccgcatg cacgagcagg acatcgacta cgcgagcgtc agcacggtcc
     3181 agcgcacctg cgagcgatga cacggttccc gccacctcga tcgacggatc ggaggcgatc
     3241 cggcgcgaga gggcagggcc ggcccattgc tcatccgcca cgatcaggac gctaatcatg
     3301 gtgcgtgcct cgctgccatt cgaatgcggg gccggagttg aaccgaacag gcgatagtac
     3361 tgtatcaggt cattttgcca gtcagcgcgc aaggaatagc gaacgggtcg gcaaggttgc
     3421 cgacccgttc gatttgatgc gattgggttg ttcgaactag cggacctgat ccatccccga
     3481 accgctgccg acgctgacgc cgttgcggcc ggtggggttg gagtcgtcga tcgagagcca
     3541 gccgcgcttg agggcagtga cgacggcttg cgtgcggtcg ttgacggcca gcttacgcag
     3601 gatcgaggtg acgtgattct tgacggtctg ggcgctgatc cccagtgcgt agccgatctc
     3661 ggcgttggtc ataccatcgc tgaccttgcg caggatctcg agctcgcggt cggtcagcgg
     3721 cgcgaaggcg ttgtgcggcg cgagatcgct cgaggtggct gagcggaact gctcgaggac
     3781 ccgggcagcc acgtacggct tgtcgagaag ctgctcgttg atgacgtact cgccggtggc
     3841 cgagcgcttg atcagcgtga tcagcttggt ctcatctatg tcctggccac agtaggccga
     3901 ggcgccggcg cggatcgcac caaagagctc atcgtccgac tcctgcgcgg cgacgacgat
     3961 gacagcaatc gacggaaagc ggcgacggaa ctcggtggcg gcattcaagc ccgggccatc
     4021 ggccagcgtt gtgccaatca gggtgacatc cggcagctgc tcatcggcgg tgcgatagcc
     4081 ttcctcggcc gtaccgaccg agccgaccaa ttgcagctca ttgtcggtcg ccaggcaccc
     4141 ggcaagccct cggcggaaga ggggttcccg ctcaacgatc aggacgcgaa tggtatcgct
     4201 cattgacatt cctcacgcgg aataggatgt ggtgcgttgt gattgaaccc tcaacccaac
     4261 gtggggccag cccgcgaatg ttggcgcaac gtactgtgcg ccatttcgct gctcccgcgc
     4321 atttccccct gcgtggagcc cagccaagct agcctcagtg tacggttacc ccggtcatag
     4381 cacaactgga ccatggtccc tgctggggac cccgaacgtc ctgtatgcgg tactctttcc
     4441 ggctcttctg catgaccagg aggtagcggc gcaccgcggc attctcggcc ggttgccgca
     4501 aaatctcccg ctacggcgcg gtaactgacg gttcgggctc gctctcgatc ggcgcgaagc
     4561 cgatgctgag ggactgctcg acgatgccgc ggcagagcag gttctcggcc tgccggagtg
     4621 gcccgatcag cccatgctcc ggtttgacat cctccagcat ctcccggacc tgtttaacca
     4681 ggtcgatcgt cttgttgaag gtaaggacga ggtcgccctc ggagagctga atcgtttcgg
     4741 tgacgtcaac catcgtcttg cccaggcacc aggcacgggc cggtccgtag aacgatgggt
     4801 tgtgcccctc cgagatgaag aggctgtgct cccgctcctc cgagatcaat gcacgctcga
     4861 tctcctcgat ccgccgcctg atgggagtga aatgcggcgg cagcacgaac tgattgccgt
     4921 agcggaagtc gcgatcgaag ctgtaccagg aaaagacctc agccagatcc tccggcgcca
     4981 gatcgttgag caggccgcga tcaatcagct cgcagagaat caacccatcg gtgtcgaaga
     5041 caccagccag catgtcggcc ttctcggtcg catagccccg gcgcatatag ccgaaccggt
     5101 gcaggacctc ccggatgccc cagaggaccg actggatccg ctgctcctcc gcttccgtct
     5161 ctcgatttag caactct
//
LOCUS       DATAOZ010000472        15395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4397503, whole genome shotgun sequence.
ACCESSION   DATAOZ010000472 DATAOZ010000000
VERSION     DATAOZ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15395
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4397503"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..921)
                     /locus_tag="VHR64_12035"
     CDS             complement(55..921)
                     /locus_tag="VHR64_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3303604.1"
                     /translation="MPLCVYRTGSDVSPRLGLIAGEQVIDVARAGGPATLSAALQLSA
                     ADLRTELGAVGQSKDSLALADVTLDVPMDEQEIWAAGVTYLRSRDARMEESTEKSVYD
                     RVYDAERPEIFFKAMRNRTSGTGTSVAIRSDSSWDVPEPELTLAINATGEIVGYTIGN
                     DVSSRTIEGENPLYLPQAKVYSKCAGLGPVIALAWELTETNDLTIKLTITRDGKSYFE
                     GETSTKQIHRPFGQLVDYLFRNNDFPSGVFLMTGTGIVPPSEFTLEDGDHVAITIQGI
                     GTLENPIVRHKR"
     gene            complement(969..2564)
                     /locus_tag="VHR64_12040"
     CDS             complement(969..2564)
                     /locus_tag="VHR64_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303605.1"
                     /translation="MSALTRPSPTAEPQTQSPRDDLKYRRRWWTLAVLSLSLLIIGLD
                     NTVLNVAIPTLQEHFSASASELQWMVDAYVVVYAGLLLTLGAAGDRYGRRKALQAGLL
                     IFGVSSFWAAYAGSANHLIAARAVMGIGGAAIMPSTLSVLSDVFPREERGRAIAIWSG
                     VAGLGIGLGPLFGGFLLEHFWWGSIFLINVPIVIIALILGRFLVPESRDEHAHPLDIP
                     GAILSISAVSMLVYAIIEAPNSGWLDTRTLLSFTVAIALGIAFFWRELHTEYPMLDLA
                     FFRNPRFSFGAAAIGVSFFALFGVVFANTQYLQFVHGYTALEAGVRLIPIAFGIMVGA
                     GNSNRFVTRAGTTRVVVAGMLIVAAVMASIGFWQVDTPYWVIGTTLFFLSVGMGNVMA
                     PSTDAVMGAVPEAKAGVGSAMNDVVRQVAGAFGVAIIGSILNTAYTRNMHDATAALPP
                     QAAEPAGNSVGAALVVASRIGGQAGEALAAAGRAAFTDAMNIAAFVAAAIALVGAVIV
                     ARNLPAHHLPLAEEPLAGDDALP"
     gene            complement(2625..3437)
                     /locus_tag="VHR64_12045"
     CDS             complement(2625..3437)
                     /locus_tag="VHR64_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3303606.1"
                     /translation="MNEQRWDELITAARSASESTGLPIADVSIDDIAKHSGLSRATLY
                     RRIGSRAALEEAVRAAGIDPGGRPDVRKRAIEAAADLIEAGGLAALTLEAVAERAECS
                     VRALHTQLGGREGLLAAVFERYSPLPHIERAMSGDPMPLEEGLRAIYGTILETAHARR
                     GLLTAVIADVAFRPDGPTAGFVRGTYLPRVTNVILPWLEVLRRSGEIRPLPIPLLIQL
                     LVGPIGAHVFLGPVLTHTTGLQPPSRDEVCDELVDAFMRTVARAPTEPTGND"
     gene            complement(3476..5653)
                     /locus_tag="VHR64_12050"
     CDS             complement(3476..5653)
                     /locus_tag="VHR64_12050"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX3303607.1"
                     /translation="MIQPFSERYQTALGNPRLAANVTRYQRNWRINRDTSLQEIEFER
                     LRTDFKATKTRVQDRLDDYLAQFQKTAEESGAIVHHAGNARDANRIALEICRAHEAKQ
                     IVKSKSMVTEEIELNHFLEGQGIECVETDLGEWIVQKAGQRPSHIVGPALHMGRQDVA
                     ELLTNKLGVPVSQEDIPEQVHAIRDLIRPLFFTAGIGMTGANALIAESGSVMMCTNEG
                     NGRMASSVPPVHIVMAGIEKLIPTFDDAVTQLRLLGRSGTGQRMTVYTTFITGPTPGH
                     EMHIILVDNGRRKMRAMPEFREALHCIRCGACANVCPPYREVGGHVFGHIYTGAIGLV
                     VTQFHHGLDAIAKPQSMCLSCNACETVCPVNIPLPQQIIDVRKMVVAKKGLAGPKRVA
                     VAVIQRPKAFNLATRIGRRAQLPVTRGGKFVRLRSVPKLNRQTKWRSMPALATKTVMD
                     RVAPGNFFPQQSAVVSNQVTGKTAAVFPGCITDRVYPEQGMAVIDILRGLGMRVMVPK
                     GLNCCGLPSSNMGDDPAAKRMAKQTIQALERAQCEFIVSGSASCVAMIMQDYIHLFRN
                     DPGWQRRAIALAGKVRDLTTFLVHDAQLQPGALHGQADDEPITYHDSCQGLNALGLAA
                     EPRYLLSDVLGVPVKELAENRLCCGFGGSFGFDYPEVSERLMNAKLDNAAATGARTVV
                     TDNQGCIMHLRGGCDAAKRPITISHIAELISTRMRQRQATPNS"
     gene            complement(5726..6298)
                     /locus_tag="VHR64_12055"
     CDS             complement(5726..6298)
                     /locus_tag="VHR64_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX3303608.1"
                     /translation="MEIDLEHSHLLIAQFAERATPLGSVVERAADSAEAAGIIASLVK
                     QAGTDAISAAPVVAERAPQLVAALTYAGIEVQTIGSPEDARDRPVGLSLASRAIAETG
                     SALLDERQLADRAASLMTLHSIVLVAASDIIPSLDSTPALLREIAGRPGGGYASFVTG
                     PSRTADIEMSLTVGVQGPGQVTILFVDSLT"
     gene            complement(6304..7887)
                     /locus_tag="VHR64_12060"
     CDS             complement(6304..7887)
                     /locus_tag="VHR64_12060"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEX3303609.1"
                     /translation="MAIEIGSRDAVRGSVPFNDVAIILDPSDAVGVAKRPLLPGTILL
                     RENGEELRISQMVPPGHKFALVDIANDAPVRRYGQIIGFATADIKPGQHVHVQNMSIK
                     EQGSLKVDYAIGTEYKPETFVPESERRTFMGFKRKDGRVGTRNYVAVLASVNCSSSAT
                     VAVVNHFKQPGALKDYPNVDGVIGYPHKGGCGAHIGSRDLQLFQRTLAGVVDHPNVAG
                     YVVLSLGCEVNQPSDMIESTTMKDDQPLVITIQQDGGFAKTVELGIEAVKRLLPEANK
                     AVREEVTADHLMVALQCGGSDGWSGVTANPALGKAADLVVQQGGTVVLGETTEVYGAE
                     HLLTRRAKSPEVAQRLIDMIHWWEDYTAYFGGTIDNNPSPGNKLGGLTTIYEKSLGAV
                     AKAGSMPLNQVVAYGERVTEKGFVHMDTPGYDPVSVTGQVAGGCNVVCFTTGRGSAFG
                     FKPSPSIKLATNSELYGKQEPDMDINCGRVLDGITLDQMGQEIFEEILAVASGTRTKS
                     ELAGVGEEEFNPWILGAML"
     gene            8326..8607
                     /locus_tag="VHR64_12065"
     CDS             8326..8607
                     /locus_tag="VHR64_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303610.1"
                     /translation="MSNASMAKEALIAGVTATVVIPLLWFLGATVNPDRDTHSPGLDL
                     VVLTLAGAVMVMLLRSTWRNTAVPSSDGDCAGEDWDWRHFSDSYGSGQC"
     gene            complement(8620..9765)
                     /locus_tag="VHR64_12070"
     CDS             complement(8620..9765)
                     /locus_tag="VHR64_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX3303611.1"
                     /translation="MKVTRIMDDGTARLLDESMSAPSFWAVIPAGGSGTRLWPLSRAA
                     RPKFLLPLVRRETSLLQDTAERIWGMTPPERTLVVCGPAHTVAIARQLPEIPEGNLIV
                     EPSPRGSGPAIILAAALIHRLDPNAIMGSFAADHDVTDRRAFEEAVRTAIRAAHEDCL
                     VTIGLTPTRPETGYGYIERSENVVVENDGSTAYEALRFVEKPPLETARQYLESGRFLW
                     NASMFIWKCSTLLEVASTLMPDLLDAINQIVEVWDTPDREDTIIRIWEALPESTIDQG
                     IMEKAQRVAVVPADIGWSDVGDWHGLGELIEQDSVGNSVRGDIVQVETKRSVVWSETG
                     RLVALIGLENVVVVDTEDALMVIDRERSQDVKKIVGELKRLSRHEFS"
     gene            9836..10762
                     /locus_tag="VHR64_12075"
     CDS             9836..10762
                     /locus_tag="VHR64_12075"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEX3303612.1"
                     /translation="MHPRLDSKPWGGDRLAHFGIALDGKTDIGEAVLTSNDAIVTQGS
                     YAGQTLGDLVALDPDALLGPVACQFRSTGFPLLAKLIDAHENLSIQVHPTDDLAPSDG
                     CGKTEAWFILEAAPGAVVYAGLRDGAALGEVRRRLETGESIVPLVRTVPVAPGDTLFL
                     PAGTIHALGGGIVLYEIQQQSAITYRLEDWGRAREMHIEAGLAALNPEHRPTPNRAGM
                     RTSTDRPRPPVNCPYFSLEILQVEPGERIKIAPEPGPQVFTCVRGETELGAGQGAVAL
                     DAGATAVLFAKVGPARIESSTGGRVLRAWVGD"
     gene            complement(10739..11116)
                     /locus_tag="VHR64_12080"
     CDS             complement(10739..11116)
                     /locus_tag="VHR64_12080"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3303613.1"
                     /translation="MEAEGFVMSLDDALKAHAAAGGAYHQFFQVPEMSAGIYTLPAGG
                     VDGQAPHVEDEIYYVISGKAQVQIGDRHYPVQTGATIFVAREVEHRFHDIEEDLTILV
                     IFAPAHDEAEADRRRASVADPGT"
     gene            11174..12454
                     /locus_tag="VHR64_12085"
     CDS             11174..12454
                     /locus_tag="VHR64_12085"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303614.1"
                     /translation="MRARFQRGVWTLHSFRLLISASMISIFGSLITSTAFPFIAIQQL
                     DAGPKELALLSLAGIVPSVVLGSIAGIWIDRLSRRLVMIASDLASAVALLTIPVAYWL
                     DALTLVHLVAVAFVTSVTRLCFRIADRSFLPQVVGREHIEEANATLSGGSAISEAGGF
                     SVGGLLVQLLTGPVALLFDAVSFVISAFLLRKIPDQPAPVDPEQPVETHHWKAELIDG
                     LRYLRRSVVLSPLAISIFTMSIGIETIGTVYFLFVNKELGFSAGALGFIFATGGIGSL
                     IGAAMSTRVTARFGAGPALIGSLVILAIGQGSIVLATSVSLFAVIVMLGQQMTDAFWL
                     YYESTSMSLRQLNAPESMLGRINGAFESIEFIGLLLGAGLGALLGETVGLRATLITGA
                     ALLAVAGLPLLLSPVRETRALAPVEADVGALVEA"
     gene            12753..13946
                     /locus_tag="VHR64_12090"
     CDS             12753..13946
                     /locus_tag="VHR64_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3303615.1"
                     /translation="MAIATSRFTGVDFYDLDSLLSEEERAVRDAVREWVDDNLMPIIG
                     DCYIEGRFPKEIIPGMAELGLFGANLPEEYGCAGLNNVAYGLIMQELERGDSGIRSFA
                     SVQGALVMYPIYTFGSDEQKSYWLPKLASGEEIGCFGLTEPDYGSNPGGMITTARETA
                     DGWVLNGVKMWITNGSQATVSIIWAKTGDVDDPKSIRGFIVPTDTKGYAARDQKGKLS
                     LRASDTSEIHLNDVHVPKDAILPKSGGVKSPLMCLTQARYGIAWGGIGAAMACYDEAL
                     RYSQQRVMFGKPIGQTQLQQARLAEMLSEITKAQLLTLQLGRLKDAGTMTPQQVSLAK
                     RNNVNMATECAREARRLLGANGILAEYHSMRHMANLESVYTYEGTHDMHTLILGQEVT
                     GLNAF"
     gene            13977..14729
                     /locus_tag="VHR64_12095"
     CDS             13977..14729
                     /locus_tag="VHR64_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX3303616.1"
                     /translation="MKIAVCIKRVPDTEMRFTIAPDGKSIDTAGLKYEISTFDEYAIE
                     AALRLNEAQGGGEITVVGLGPAGLGELLRRGLSMGADKAIHLTADEVPVDGFAIARAL
                     AAALRDGGYDLILFGRNATDTGHGTVGPMVAQLLGLPCVTAITHLDVNGGTGTAQRAL
                     EGATETVSFPLPAVLSIDEGLGRPRIPSLKGIMAAKKKPLDEQPAQLGPVSLTLESLA
                     PPPERPAGRIVGEGAAAVPELVRLLREEAKVL"
     gene            14729..>15395
                     /locus_tag="VHR64_12100"
     CDS             14729..>15395
                     /locus_tag="VHR64_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303617.1"
                     /translation="MADVFVFAESRGGALRQVALETVAAARQAADAIGGEVHAMVAGA
                     PGIGQVADALGQHGADVVLVVEDAALERYSPEVVAATAAARIKDGGYRVALFAASAEG
                     HDLAPRVAAILGVSMASDVIDFAVDGDAVTVKHPAYTGKAIATLKLTGTPAVISVRPG
                     AYAATDAARSARVEAVQPAIDPGSARVTVTEIGTKTAKVDLAEAPVIVVGGRGLQEAG
                     NFAL"
BASE COUNT         2511 a         4737 c         5447 g         2700 t
ORIGIN      
        1 gggccatgcc ggccatgggg ggccggcgct acgtcatttc gttcagcgat ccggctagcg
       61 cttgtggcgg acgatggggt tttcgagggt gccgatgcct tggatggtga tggcgacgtg
      121 gtcgccgtcc tcgagcgtga actcggaggg cgggacgatg ccggtgccgg tcatcaggaa
      181 gacgccgctc gggaagtcgt tgttgcggaa gaggtaatcg acgagctgcc cgaacgggcg
      241 gtggatctgc ttcgttgagg tctcgccctc gaagtagctc ttgccgtcgc gggtgatcgt
      301 cagcttgatg gtgaggtcat tggtctcggt cagctcccag gcgagtgcga ttaccgggcc
      361 gaggccggcg cacttcgagt agaccttggc ttgcggcaga tagagcgggt tctcgccctc
      421 gatcgtgcgg gaagagacat cgttgccgat cgtgtagccg acgatctcgc ctgtcgcgtt
      481 gatggcgagg gtgagctctg gctcgggcac gtcccatgag gaatcgctgc ggattgcgac
      541 cgacgtgccg gtgccggatg tgcggttgcg catcgccttg aagaagatct cgggtcgctc
      601 ggcatcatag acgcggtcgt agacgctttt ctcagtcgat tcctccatgc gggcgtcgcg
      661 gctgcgcaga taggtgactc cggcggccca gatctcctgc tcgtccatcg gcacgtcgag
      721 cgtgacgtcg gcgagggcca gcgagtcctt cgattggccg accgcgccga gttcggtgcg
      781 gaggtcggca gcagagagct ggagtgcggc tgagagggtg gccggtccgc ctgctcgggc
      841 gacatcgatt acctgctctc cggcaatcag gcccaatcgc ggcgagacgt cactgccggt
      901 tcgatagaca caaagcggca cccggtgctc cttcctatgg cgtggatcga tccgtcttgc
      961 cgggcatcct acggcaaagc gtcgtcccct gccaaaggct cctcggcgag cgggagatga
     1021 tgggccggga gattgcgggc cacgatgacg gcgccgacca acgcgattgc ggcagccacg
     1081 aacgcggcga tgttcatcgc atcggtgaag gcggcgcgtc cggccgctgc caacgcctcg
     1141 cccgcctgtc cgccgattcg cgaggcgacg acgagcgcgg ctccgaccga gttcccggcc
     1201 ggttcggccg cctggggtgg gagcgctgcg gtcgcgtcgt gcatgttccg ggtgtaggcc
     1261 gtgttcagga tcgagccgat gatcgcgacg ccgaacgcgc cagcaacctg ccggacgaca
     1321 tcgttcatgg ccgagccgac acctgccttt gcctcgggca ccgcgcccat gaccgcatcg
     1381 gtcgacggcg ccatcacgtt gcccattccg accgaaagga agaagagcgt cgtgccgatc
     1441 acccagtaag gcgtatcgac ctgccagaag ccgatgctgg ccatgacggc cgccacgatc
     1501 agcatgccgg cgacgacgac ccgcgtcgtg ccggcgcggg tgacgaagcg attgctgttg
     1561 ccggcgccaa ccatgattcc gaaggcgatc ggaatcaggc gcaccccggc ttcgagcgcg
     1621 gtgtacccgt ggacgaactg gaggtactgc gtatttgcga atacgacgcc gaacagagcg
     1681 aagaagctca cgccgatcgc cgccgcgccg aaggagaagc gcgggttgcg gaagaaggca
     1741 agatcgagca tcgggtattc ggtgtgcagc tcgcgccaga agaaggcgat accaagcgcg
     1801 atcgcgaccg tgaagctgag aagcgtgcgg gtgtcgagcc agccactgtt cggcgcctcg
     1861 atgatcgcgt agaccagcat ggaaacggct gagatcgaaa ggatcgcgcc ggggatatcg
     1921 agcggatggg cgtgctcatc gcgactctcc ggaacgagga accgacccag gatcagggcg
     1981 atgatcacga tcgggacatt gatcagaaag atgctgcccc accagaagtg ttcgagcagg
     2041 aacccgccga agagcggacc gagcccgatc ccgagtccgg cgacaccgga ccagatcgcg
     2101 atcgcccggc cccgttcctc gcgcggaaag acatcgctca ggaccgagag ggttgagggc
     2161 atgatggccg cgccgccgat ccccattacc gctcgggcgg cgatcagatg gttggccgag
     2221 ccggcatagg cggcccagaa gctggacacg ccgaagatga gcaggccggc ctggagcgcc
     2281 ttgcgccgcc cgtagcgatc tccagccgcg ccgagggtca ggagcaaacc ggcgtagacg
     2341 acgacgtacg catcgaccat ccactgcagc tcagaggcgg aggccgagaa gtgctcctgg
     2401 agggtcggga tggcaacatt caggaccgtg ttgtcgagac cgatgatcag caggctgagc
     2461 gagaggacgg cgagcgtcca ccagcgccgg cggtatttca ggtcgtcgcg cggcgactgg
     2521 gtttgcggct ccgcggtcgg ggatggacgg gtcagtgcag acatgattcc actctcctcg
     2581 catgagcgtg gcgttacaca gatcgatacg aatggtggcg gcgttcagtc gtttccggtg
     2641 ggttcggttg gagcccgcgc aacagtccgc atgaaggcgt cgaccaactc gtcgcagacc
     2701 tcatcccgcg acggcggctg caatccggtc gtgtgcgtca gtaccggtcc gaggaacacg
     2761 tgggcgccga tcgggccgac caggagctgg atcaggagcg gaatcggcag cggccggatc
     2821 tctcccgatc tccgcagaac ctcgagccac ggcaggatga cattggtgac acgcggcagg
     2881 taggtgccgc ggacgaaccc ggccgttgga ccatcgggcc gaaacgcgac gtcggcaatc
     2941 acggcagtca gcaaccctcg ccgggcatgg gcagtctcga ggatcgtgcc atagatcgcg
     3001 cgcagaccct cctccagggg catcgggtcg ccggacatgg cgcgttcgat gtggggcaga
     3061 gggctatatc gctcgaagac ggctgccagc agaccttcgc gaccgccgag ctgggtatgc
     3121 agcgcgcgca ccgagcattc ggcacgctcg gccacggcct cgagggtcag ggcggcgagg
     3181 ccaccggctt cgatcaggtc ggcagcggcc tcaatcgcgc gtttgcggac atcaggacga
     3241 ccgccggggt cgatcccggc ggcgcggact gcctcttcca acgcggcgcg cgacccgatt
     3301 cgtcggtaga gcgtagcgcg ggagagcccg gagtgtttcg cgatgtcgtc gatggacaca
     3361 tcggcgatcg ggaggccagt cgactcgctc gccgaccgcg ccgcggttat cagttcgtcc
     3421 caacgctgct cattcatgcg acgatactac acttcacctg tagtgtcgtc aagtatcagc
     3481 tgttcggcgt tgcctggcgt tgcctcatgc gagtggagat gagctcggcg atgtggctga
     3541 tggtgattgg gcgtttggcg gcgtcgcagc cgccgcggag gtgcatgatg cagccctggt
     3601 tgtcggtgac gacggtgcgg gcgccggttg cggcggcgtt gtcgagcttg gcgttcatca
     3661 gccgctcgga gacctccggg tagtcgaagc caaacgatcc gccgaagccg cagcagagcc
     3721 gattctcggc cagctccttg acggggacac cgaggacgtc ggagagcagg tagcgcggtt
     3781 ccgcggccag gccgagcgcg ttcagcccct ggcaggagtc gtgatacgtg atcggctcat
     3841 cgtccgcctg tccgtgcagg gcgccgggct ggagctgggc gtcgtgaacc aggaaggtgg
     3901 tcaggtcgcg caccttgccg gccagggcaa tcgcgcggcg ctgccagccg gggtcattgc
     3961 ggaagaggtg gatatagtcc tgcatgatca tcgccacgca actcgcgctg ccggagacga
     4021 tgaattcgca ctgcgcccgc tcgagggcct ggatcgtctg cttggccatg cgtttcgcgg
     4081 ccgggtcgtc ccccatgttg ctcgatggca ggccgcagca gttcagcccc ttcgggacca
     4141 tcacccgcat gccgaggccg cgcaatatgt caatcacggc catgccctgc tccgggtaga
     4201 cccgatcggt aatgcagccg gggaagaccg cggccgtctt gcccgtgacc tggttcgaaa
     4261 cgaccgccga ctgctggggg aagaagttgc ccggcgcgac gcggtccatg accgtcttgg
     4321 tcgccagggc tggcatcgat cgccatttgg tctgccggtt gagcttgggc acgctgcgca
     4381 ggcggacgaa cttgccgccg cgggtgaccg ggagctgggc acggcggccg atccgggttg
     4441 ccaggttgaa ggccttcggg cgctggatga ccgcgaccgc gacccgcttc ggtcccgcca
     4501 ggcccttctt cgcgacaacc atcttgcgga cgtcgatgat ctgctgcggg agcgggatat
     4561 tgaccgggca aaccgtctcg caggcattgc aggagaggca catgctctgc ggcttggcga
     4621 tcgcgtcgag gccgtggtgg aactgggtga cgacgaggcc gatcgcgccg gtgtagatat
     4681 ggccgaagac atggccaccg acttcgcggt atggcgggca gacattggcg caggcgccgc
     4741 agcggatgca gtgcagggct tcgcggaact cgggcatcgc gcgcatcttg cggcggccgt
     4801 tgtcgaccag gatgatgtgc atctcgtgac cgggcgtcgg tccggtgatg aaggtcgtgt
     4861 agacggtcat gcgctggccg gtgccgctgc ggcccagcag gcgaagctgg gtgacggcgt
     4921 cgtcgaaggt cgggatcagc ttctcgatgc cggccatgac gatgtggacc ggcggcacgg
     4981 acgaggccat gcggccgttg ccctcgttgg tgcacatcat gacgctgccc gattcggcga
     5041 tgagggcgtt ggcgccggtc atgccgatcc cggctgtgaa gaagagcggc cggatcaggt
     5101 cgcgaatggc gtggacctgt tcggggatgt cctcctgcga gacgggcact ccgagcttgt
     5161 tggtgagcag ctcggcgaca tcctgccggc ccatgtgaag agccgggccg acgatgtggc
     5221 tcgggcgctg tccggccttc tggacgatcc actcgccgag gtcggtctcg acgcactcga
     5281 tgccctggcc ctccaagaag tgattgagct caatctcctc ggtgaccatc gacttggact
     5341 tgacgatctg cttcgcctca tgggcgcggc agatctcgag ggcgattcgg ttggcatcgc
     5401 gagcattacc ggcgtggtgg acgatcgctc cggactcctc ggccgtcttc tggaactggg
     5461 cgaggtagtc gtcgaggcgg tcctggacgc gggtcttggt cgccttgaag tcggtccgca
     5521 ggcgctcgaa ctcgatctcc tggagcgagg tgtcgcggtt gatgcgccag ttgcgctggt
     5581 agcgggtgac attggcggcc aggcgcgggt tgccgagggc ggtctggtac cgctcagaga
     5641 acggctggat cacgtgcgga tcatcgtgct cgtgctgtga tcgtgcgtga ctttcctgaa
     5701 ccgagaccat ggtgcgccgc tttcgttacg ttagtgagtc cacgaacagg atagtgacct
     5761 ggccggggcc ttgcacgccg acggtgagcg acatctcgat gtcggcggtg cggcttgggc
     5821 cagtgacaaa gctggcgtag ccgccacccg gccggccggc gatctcgcgg agcagggccg
     5881 gggtgctgtc caggctcggg atgatgtcag aggcggcgac caggacgatg ctgtgcaggg
     5941 tcatcaggct ggcggcgcga tcggccaact ggcgctcgtc gagcagggct gaaccggtct
     6001 cggcaattgc gcgagatgcc agcgagaggc cgacgggtcg atcccgcgca tcctcaggcg
     6061 agccgatggt ctgcacctcg atcccggcgt aggtcagcgc ggcgacaagc tgtggcgcgc
     6121 gctcggccac gaccggcgcg gccgagatgg cgtccgttcc ggcctgtttg acgagtgagg
     6181 caatgatgcc ggcggcttcg gcactatccg cggcacgctc gacgactgag ccgagcggtg
     6241 ttgcccgctc ggcgaactgc gcgatcagga ggtgcgaatg ctcgagatcg atctccatcc
     6301 gggctagagc attgcgccca ggatccaggg gttgaactct tcctcgccca cgccggccag
     6361 ctcgctcttg gtgcgcgtgc cggacgcgac ggccaggatc tcctcgaaga tctcctgccc
     6421 catctgatcg agcgtgatgc catcgagcac gcggccgcag ttgatgtcca tgtcgggctc
     6481 ctgcttgccg tagagctcgc tgttggtggc cagcttgatg gaaggggacg gcttgaatcc
     6541 gaaggccgag ccgcggccgg tcgtgaagca gacgacattg cagccgccgg cgacctggcc
     6601 ggtgaccgag accggatcgt agccgggcgt gtccatgtgg acgaaaccct tctcggtgac
     6661 gcgctcgccg taggcgacga cctggttgag cggcatcgat ccggccttgg cgaccgcacc
     6721 gagcgacttc tcgtagatcg tggtcaggcc accgagcttg ttgcccggtg aggggttgtt
     6781 gtcgatcgtg ccgccgaagt aggcggtgta gtcctcccac cagtggatca tgtcgatcag
     6841 ccgctgggcg acctccgggc tcttcgcgcg ccgggtgagc aggtgctcgg cgccgtagac
     6901 ctcggtggtc tcgccgagga cgaccgtgcc gccctgctgg acgaccagat cggccgcctt
     6961 gccgagggcc gggttggcgg tcacgccgga ccaaccgtcg gagccgccgc actggagcgc
     7021 gaccatcagg tgatcggccg tgacctcctc gcggaccgcc ttgttagcct ccgggagcag
     7081 ccgcttgacc gcctcgatgc cgagctcgac cgtcttcgcg aagccgccat cctgctggat
     7141 cgtgatgacc agcggctggt cgtccttcat ggtggtcgac tcgatcatgt ctgacggctg
     7201 attgacctcg caaccgaggc tgaggacgac gtagccggcc acgttcggat gatcgacaac
     7261 gcctgccagc gtgcgctgga agagctggag atcgcgcgag ccgatgtgcg cgccgcatcc
     7321 gcccttgtgc gggtagccga tcacgccgtc gacgttcggg tagtccttga gcgcgccggg
     7381 ctgcttgaaa tggttgacca ccgccacggt cgccgacgac gagcaattga ccgaggcgag
     7441 cacagcgacg taattgcggg tgccgacccg gccgtccttg cgcttgaagc ccatgaaggt
     7501 gcgccgctcg gattccggca cgaaggtctc gggcttgtac tccgtgccga tcgcgtagtc
     7561 gactttgagc gagccctgct ccttgatcga catgttctgg acatggacgt gctggccggg
     7621 tttgatgtcg gccgtggcga agccgatgat ctggccgtag cggcggaccg gcgcatcgtt
     7681 ggcgatgtcg acaagggcga acttgtggcc cggcgggacc atctgagaga tgcgcagctc
     7741 ctcgccattc tcgcggagca ggatcgtgcc cgggagcagc gggcgcttgg cgacgccgac
     7801 cgcgtcggac gggtcgagga tgatggcgac gtcgttgaag gggacgctgc cgcggaccgc
     7861 gtctcgggat ccgatttcga ttgccatgat gggatggacc tctccgcctt gatgggtcgg
     7921 gtgattccgg gaccggccgg cgcagcaagg caatctgcgg cgggcgacac atccggatcc
     7981 tggttgggct cgtgcaaacc aaccaccggc ggcgtcagaa cgccacggcg ggcctggcga
     8041 gcgcaccgtc aatgctttcc aaatgatatc ccagcagaga ttgaatcgga cgcggtccgg
     8101 agcgaatgtg cggtccagga gtgcgccagc acattggagc agggggcggg cccgggcaga
     8161 ttcgcatcct caaccggttc gcatgatgtc gattcgtggc tcgcccagac gactatgtgg
     8221 tgaaggtagt tcccgacagt catcgagtcc ggatctcaca caggtcacga aaccgcacca
     8281 accgtccagt ctgaaaacga tccctgcgat agagaggagc acaccatgtc gaacgcgtcg
     8341 atggccaagg aagcattaat tgccggcgtc accgcgaccg tggttatccc gttgctctgg
     8401 ttcctgggcg cgaccgtgaa tcccgaccgg gacacccatt cacccggtct cgacctggtc
     8461 gtcctgacgc tggcgggcgc ggttatggtc atgctgctgc ggtcaacctg gcgaaacacc
     8521 gcagttccat cgtcggacgg cgattgcgcg ggcgaggact gggattggcg ccattttagc
     8581 gactcgtacg gaagcggaca gtgctaaccg gatcgaacgc tagctgaatt cgtggcggct
     8641 gaggcgcttg agctcgccga cgatcttctt gacgtcctgc gagcgttcgc ggtcgatgac
     8701 catcaacgcg tcttcggtat cgacgacgac cacgttctcg aggccgatca gcgcgaccag
     8761 ccggcccgtt tcggaccaga cgacactgcg cttggtctcg acctggacga tgtcgccgcg
     8821 gacgctgttg ccgaccgagt cttgctcgat caactcgccg aggccgtgcc agtcgccgac
     8881 atcgctccag ccgatgtcgg ccggaacgac cgcgacccgc tgggccttct ccatgatgcc
     8941 ctgatcgatc gtcgattcgg ggagcgcctc ccagatgcgg atgatcgtgt cctcgcggtc
     9001 cggtgtatcc cagacttcga cgatctgatt gatggcgtcg aggaggtccg gcatcagggt
     9061 gctggcaact tcgagcaggg tcgagcactt ccagatgaac atgcttgcgt tccagaggaa
     9121 gcggccgctc tcgaggtatt gccgggccgt ctcgagcggc ggcttctcga cgaagcggag
     9181 ggcctcgtag gcggtcgacc catcattctc gacgacgacg ttttcgctgc gctcgatgta
     9241 gccgtagccg gtttctggcc gggtcggggt caggccgatc gtgacgaggc agtcctcgtg
     9301 cgcggcgcgg attgcggtcc gcactgcctc ctcgaatgcc cgccggtcgg tcacgtcgtg
     9361 atcggcggcg aagctaccca tgatcgcgtt cgggtcgagc cggtggatca gcgcggcggc
     9421 caggatgatg gctggcccgg aaccgcgtgg agagggctcg acgatcaggt tgccctccgg
     9481 gatctcgggc aactggcggg cgatcgccac cgtgtgggcc ggaccgcaga cgaccaacgt
     9541 ccgctccggt ggtgtcatgc cccagatccg ctcggccgta tcctggagca gcgacgtctc
     9601 ccggcggacg agcgggagca ggaacttggg ccgcgcggct cggctgagcg gccagagccg
     9661 ggtgccggag ccgccggccg ggatcacggc ccaaaatgat ggcgcggaca ttgattcatc
     9721 gaggagccgg gcagtgccgt cgtccatgat tcgtgtcact ttcatttgac agtcgcatct
     9781 ctgctcgaca ttatgcatgc ctatggaaga gacacgaata tctgagcccc tgttggtgca
     9841 cccgcgcctg gattccaagc cgtggggcgg ggaccggctt gcccacttcg ggattgcgct
     9901 cgatggcaaa accgacatcg gcgaggcggt cctgaccagc aacgacgcca tcgtcaccca
     9961 aggttcatac gccgggcaga cactgggcga tctggtcgcg ctggatcccg acgctctcct
    10021 cggcccggtt gcgtgtcagt ttcgcagcac cgggtttccg ctgcttgcca agttgatcga
    10081 cgcgcacgag aacctctcga tccaggtcca cccgaccgac gaccttgcgc cctcggatgg
    10141 ctgcggcaag accgaggcgt ggttcatcct ggaggcggct ccgggcgcgg tggtctacgc
    10201 cgggcttcgg gacggcgccg cgctcggcga ggtgcgcagg cggctggaga ccggcgaatc
    10261 gatcgtgccg ctggtgcgaa cggtcccggt tgcgccgggc gacacgctct tcctgccggc
    10321 tgggacgatc cacgcgcttg gcggcggaat tgtcctctac gaaatccagc agcagtcggc
    10381 gatcacctac cggctcgagg actggggccg ggcgcgggag atgcacatcg aggccggact
    10441 ggccgcactg aatccggagc atcgaccgac gccgaaccgg gccgggatgc gaaccagcac
    10501 cgatcgaccg cgaccgcccg tcaattgccc gtacttctcg ctcgagatcc tgcaggtcga
    10561 gccgggcgag cggatcaaga tcgcgccgga accgggcccg caggtcttta cgtgcgtgcg
    10621 cggtgagacc gaactcggcg ccgggcaggg cgcggtggcg ctcgatgccg gggcgaccgc
    10681 cgtgctcttc gccaaagtcg ggccggcgcg gatcgagagc tcgaccggag gccgcgtcct
    10741 acgtgcctgg gtcggcgact gaggcgcggc gacgatcggc ctcggcctcg tcgtgggccg
    10801 gggcgaagat gaccaggatc gtcaggtcct cctcgatgtc gtggaagcga tgctcgacct
    10861 cgcgggcgac aaagatcgtc gcgccggtct ggacgggata gtggcgatcg ccgatctgga
    10921 cctgcgcctt gccggagatg acgtagtaga tctcgtcctc gacgtgcggg gcttgcccat
    10981 cgacgccacc ggctggtaac gtgtagatgc cagcgctcat ctcgggaacc tgaaagaact
    11041 gatggtacgc gccgcctgcc gcggcgtggg ccttcagcgc atcgtcgagc gacatgacga
    11101 acccctcggc ttccacgacc tcacctttct gctagactcg ccggccgcaa tcatagcgaa
    11161 gcacggatgg ccaatgagag cccgatttca gcgcggcgtc tggacgctgc actccttccg
    11221 actgctgatt tctgcgtcga tgatctcgat cttcgggtcg ctgatcacgt cgaccgcatt
    11281 cccgttcatc gcgatccagc agctcgatgc cgggccaaag gagttggccc tgctcagcct
    11341 ggccgggatc gtgccgagtg tcgtgctcgg ctctatcgcg gggatctgga tcgaccggtt
    11401 gtcgcgacgg ttggtcatga tcgcgtccga tctggccagc gcagtggccc tgctgacgat
    11461 cccggtcgct tactggctcg acgcgctgac cctggtccat ctggtcgcgg ttgcgttcgt
    11521 caccagcgtg acccggctgt gcttccggat agcggaccgc tcgttcctgc cgcaggtggt
    11581 cgggcgggag catatcgagg aggcgaacgc gacgctctct ggcggatctg cgatctcgga
    11641 agcgggcgga ttcagcgtcg gcggattgct cgtgcagctg ttgaccgggc cggtcgcgtt
    11701 gcttttcgat gcggtcagct tcgtgatctc ggcgttcctg ttgcggaaga tcccggacca
    11761 gccggcgccg gttgatccgg aacagccggt cgagacgcac cactggaagg cggagctgat
    11821 cgacggtttg cggtacctgc ggcggtcggt ggtgctttcg ccgctggcga tctcgatctt
    11881 cacgatgtcg atcggaatcg agacgatcgg taccgtctat ttcctgttcg tgaacaagga
    11941 gctcggcttc tcggccgggg cgctcggctt catcttcgcg accggcggga tcgggtcgct
    12001 gatcggcgcg gcgatgtcaa cgcgggtcac ggcgcggttc ggggcgggtc cggcactgat
    12061 cgggtcgctg gtgatcctgg cgatcgggca gggatcgatc gtgctggcga catcggtcag
    12121 cctgttcgcg gtgatcgtga tgctcggcca gcagatgacc gatgcgttct ggctgtatta
    12181 cgagtcgacc tcgatgtcgc tgcggcagct caacgcgccg gaatcgatgc tggggaggat
    12241 caacggggcg ttcgagagca tcgaattcat cgggctcctg ctcggcgcgg gtctcggtgc
    12301 gctgcttggc gagacagtcg ggttgcgagc gacgttgatt acgggtgcgg cgttgctggc
    12361 ggtggccggg ttgccgctcc tgctatcgcc ggtgcgggag acgcgggcgc tggcaccggt
    12421 agaggcggac gtcggggcgc tcgtcgaggc gtagcgcggc ccgcccgtgg gctgctttgc
    12481 tttcggaaga cccatttcgg gaggcacaac ggctctgtcg accgggtatg cggtcacatc
    12541 agccggccac ggggggccgg cgctacgcct tggtctccgg tgttcgcgag ttgccaggtt
    12601 gggaagcgtc gggcagatgc catctgcccc tacatcggtt cttttccctc aaccctccac
    12661 cctcaactct cagccgtttc gctcgcctcg cggtacaatc cctcgttgga ttgcggacca
    12721 tttcgcagcc ttacctacca atggagacct gaatggccat cgcgacctcc agattcactg
    12781 gtgttgactt ctacgacctc gactccctgc tctcggaaga ggagcgggcc gtgcgcgacg
    12841 cggttcgcga gtgggtcgat gacaatttga tgccgatcat cggcgactgc tacatcgagg
    12901 ggcgcttccc gaaggagatc atcccgggga tggccgagct cgggctcttt ggcgcgaacc
    12961 tgcccgagga gtacggttgc gccggcctga acaatgtcgc ctacggtttg atcatgcagg
    13021 aactcgaacg cggcgactcg ggcatccgct ccttcgcgtc ggtgcagggc gcgctggtca
    13081 tgtatccgat ctacacgttc gggtcggacg agcagaagtc gtactggctg ccgaagctgg
    13141 catcgggtga ggagatcggc tgcttcggcc tgaccgagcc ggattacggc tcgaacccgg
    13201 gcggcatgat cacgacggcg cgggagaccg cagacggctg ggtcctgaac ggcgtcaaga
    13261 tgtggatcac gaacggctcg caggcgacgg tctcgatcat ctgggccaag accggcgacg
    13321 tcgatgatcc gaagtcgatc cgcggcttca tcgtcccgac cgacaccaag ggctacgcgg
    13381 cgcgcgacca gaagggcaag ctgtcgctgc gggcgagcga cacctccgag atccacctca
    13441 acgacgtcca cgtgccgaag gacgcgatcc tgccgaagtc gggcggggtc aagagcccgc
    13501 tgatgtgcct gacgcaggcc cggtacggca ttgcctgggg cgggatcggc gcggcgatgg
    13561 cgtgctatga cgaggcactg cgctactcgc agcagcgggt gatgtttggc aagccgattg
    13621 gccagaccca gctccagcag gcgcgcctgg ctgagatgct gtccgagatc accaaggcgc
    13681 agctcctgac cttgcagctc ggccggctca aggatgccgg gacgatgacg ccgcagcagg
    13741 tctcactggc caagcggaac aacgtcaaca tggcgaccga gtgcgcgcgg gaagcccgcc
    13801 ggttgctggg cgcgaacggg atcctggcgg agtaccactc gatgcggcac atggccaacc
    13861 tcgagtcggt ctacacctac gagggcaccc acgacatgca caccctgatc ctcggtcagg
    13921 aagtgaccgg cctgaacgcc ttctagggca gcacgcacaa cgagggaatt gaccagatga
    13981 agattgccgt ctgtatcaag cgcgtccccg atacggagat gcgcttcacg atcgcgcctg
    14041 acggaaagtc gatcgatacc gccggtctca agtacgagat cagcacgttc gacgagtacg
    14101 ccatcgaggc ggcgctgcgg ctgaacgagg cgcaaggcgg tggcgagatc acggtagtcg
    14161 ggctgggacc ggccggtctc ggcgagctgc tccggcgcgg gttgagtatg ggcgcggaca
    14221 aggcgattca cctgacggcg gacgaagttc cggtcgatgg cttcgcgatc gcccgggcgc
    14281 tggcggcggc gctgcgcgat ggcggctacg atctgatcct gttcggccgg aatgcgaccg
    14341 ataccgggca cggcacagtc ggcccgatgg tcgcgcaact gctcggtctg ccctgcgtga
    14401 cggcgatcac gcatctcgac gtcaacggcg ggacgggaac ggcgcagcgg gcgctcgaag
    14461 gcgcgactga gacggtcagc ttcccgctgc cggcggtgct gagcatcgac gagggcttgg
    14521 gccggccgcg catcccatcg ctgaagggga tcatggcggc gaagaagaag ccgctcgacg
    14581 agcagccggc gcagcttggt ccagtctcat tgaccctcga atcgctggcg ccgccaccgg
    14641 aacggccggc gggtcggatc gtcggggagg gagctgcggc ggtccccgaa ctcgtgcggc
    14701 tgctccgtga agaggcgaag gtgctctgat ggctgacgtg ttcgttttcg ccgagtcgcg
    14761 gggcggcgcg ctccggcagg ttgcgctcga gacggttgcg gccgcgcggc aggcggccga
    14821 tgcgatcggc ggcgaggtcc acgcgatggt cgccggagcg ccggggatcg gtcaggttgc
    14881 cgatgcgctg gggcagcatg gcgctgatgt cgtgctggtg gtcgaagacg cggcgctcga
    14941 gcggtacagc ccggaagtgg tcgccgcgac cgcggcagcg cggatcaagg acggcggata
    15001 tcgggtcgcg ctctttgcgg cgtcggctga ggggcacgat ttggccccgc gggtggcggc
    15061 gattctgggc gtgagcatgg cgtcggacgt gatcgatttc gcggtcgatg gcgatgcggt
    15121 cacggtcaag catcccgcct acaccggcaa ggcgatcgcg acgctgaagc tgaccggcac
    15181 gccggcggtg atctccgtgc gaccgggcgc atatgcggcg actgacgcgg cgcggtcggc
    15241 acgagtcgaa gcggtgcagc cggcgatcga cccgggatcg gcgcgcgtca cggtgacgga
    15301 gatcggcacg aagacggcca aggtcgatct ggccgaggcg ccggtgatcg tggtcggcgg
    15361 gcgcggcttg caggaggccg gcaatttcgc gctgg
//
LOCUS       DATAOZ010000473         7544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_195860, whole genome shotgun sequence.
ACCESSION   DATAOZ010000473 DATAOZ010000000
VERSION     DATAOZ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195860"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VHR64_12105"
     CDS             complement(<1..751)
                     /locus_tag="VHR64_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303618.1"
                     /translation="MTEQEYQTDVTQRPVRRRGLTVNRKFTAGGLDPYSSVSWSKRTS
                     RITNPDGTVVFEMADAEVPATWSQVAADIMVSKYFRKAGVPLVDDEGNPVLDENGVQK
                     TGPERSARQVIHRLAGCWRYWGEQYGYFNSEADAQAFYDELAYMLVNQMAAPNSPQWF
                     NTGLNYAYDITGPAQGHYYVDDATGEVRLSEDAYTHPQPHACQPYDALISTPAGPVRI
                     GDIMTGNRVGLEVYDGTADGAGTTRVVAVKAN"
     gene            complement(1455..2309)
                     /locus_tag="VHR64_12110"
     CDS             complement(1455..2309)
                     /locus_tag="VHR64_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX3303619.1"
                     /translation="MIEPEQAIESTGIRSTWMIALASVIGLGVYLYPFALPTIHQTAS
                     GSTRAGEAPIVLCGLILLCLAAILREIDPMRNGEGAARIVALLASMVAIGAVARLIPS
                     FLGASPIFLLVILGGYVFGPAFGFQLGALTLAVSAFLTGGIGPWVPYQMLGVGWVGML
                     AGLLPKPARRERQLAILAGYGAVSGLIFGALLNLWFWPFAAPGGGDVSPGLSWSPDLS
                     LAEALGRYLRFYVVTSLWFDIFRSMGNVVLILALGRPILGTLERYRLRFTWQRWAAEP
                     TIEARPLA"
     gene            2358..3908
                     /locus_tag="VHR64_12115"
     CDS             2358..3908
                     /locus_tag="VHR64_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3303620.1"
                     /translation="MVVGITFHQPTTIHHPLGAMADITIQDLLVWEPRLQLVRRPLPG
                     MSANNDLEEREISWAVTIRASAPMLQPLRGGELVLLPERIVSESGLAIPVLLRELSGL
                     HAGAAVLERAPEVASPIPVLIAPEVTVEFETGLNRMLTERRGELYRAGTDLGRTLAGI
                     ASTRDLAAIIRTAGEFLDCPVALMSARGTILEQTGNAMLPSNAVRAVQSMNAPREWRD
                     QRYLVSLAGGGVLWFGPVPAERRALVRLAADRIALAAEGVLRRTVEERPRGPARSSAL
                     AAVLQGSAEEAARSGALLDLAPDATYRVLLTGAETDTGHLERALQSLGTAHSAGTMED
                     QTAVVIEARAERDRRTSDAALDRALGGAGCDWLAASASVRGIGQLPEAYRQVRYLAAL
                     MASGAIAAGAVQFEQLADVGVYRLLYELWGTPALASYIEDALGPLRAGDKRGILRETY
                     LAYLTAGGSQTETAAALGIHRNTLTYRLRQIAQLLGHDPDDPRLHLAMHLALVAERLP
                     GELRVEGT"
     gene            4064..5260
                     /locus_tag="VHR64_12120"
     CDS             4064..5260
                     /locus_tag="VHR64_12120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303621.1"
                     /translation="MATTTIPESMTHAEVRRAQRDAWRAIAAIFLISGFALSSWISRI
                     PTITDKLDLSTGHVGTALMAIAAGAIVAFPTTGRFVDTLGSARTLQIFGLIMLCTLPV
                     IGLAPNLLILIPILFVFGAGNGGLDVSMNAQGVTVEKFVGRNIMNSLHGFFSIGTFAG
                     AMLGAGAATLDIPPFAHFLVVTAIGLTVLWRVRDWLQPDPQETQAKEPAPAFALPPRP
                     IWLLGALAICVSMSEGAMADWSGLYLHDYLGTSSGFAALGFAAFSVFMLIGRFTGDRM
                     VALFGAARMVRSGAILAAVGLGIAVLINEPVAMLAGFGAVGLGLSVAYPLVFSAAGNH
                     PTLSAGRAVAGVATLGYAGFLAGPPLLGWLAEPTSLRVVMAVIVCLAALTAVLAPAVR
                     IATKDG"
     gene            5426..5638
                     /locus_tag="VHR64_12125"
     CDS             5426..5638
                     /locus_tag="VHR64_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303622.1"
                     /translation="MADKPLKKALDLSEKIVRGVDEGQSEDAPIDLPGTHRERGAENP
                     ERVVDKRTGQVEKPDKDPDNLIDRNR"
     gene            complement(5716..6879)
                     /locus_tag="VHR64_12130"
     CDS             complement(5716..6879)
                     /locus_tag="VHR64_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303623.1"
                     /translation="MHLPDRSGPSRVSRYWTALVRGESSGRLAALAADLDPAMVTMLD
                     RMRVAQHDDFPDPAFVDTLEEHLMNVSRTIYSPVASSLIDSRIATEQRGVRPIIDLPQ
                     LVRHRLSAVVAAAILLVALGGIVGFWLLDQGGSGSHTGAPAIFAPATPSPAGEATDGT
                     LVDTVLPAGTLPNGGPTAEDTSVFVLATVPPNTHGTWTPATGDCQCEGIRLISLVSGS
                     LVIRADGPVQVFRSDANGTAEAISAGSDATLGAGDTILFASDVPYKTTNSQSTPARVV
                     YWVKVGLSLMHDNAAWPNEWQVTETYDYSGVGGAIDPALSGAVTLHLQRETVEPGATS
                     KLLLTDTWFLVTDPELSQYGTNSDKSITNVTDKPVTYYAITLKPSQDIGTPAP"
     gene            complement(6866..>7544)
                     /locus_tag="VHR64_12135"
     CDS             complement(6866..>7544)
                     /locus_tag="VHR64_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3303624.1"
                     /translation="AADTYFLNAFTWIVRGVEGKRMLRNPTVAQPIPAGDAVDSMPML
                     VARSRAGDSAAFALLYRQFVNQVYAYSVRRLSTREAAEEATQETFTRALAGLNRCRND
                     AAFAGWLFGIAHHVVNEQYRAAGLQTSSLDGNLDPADPESGPEDRAICNEDAAELRRA
                     RERCLTEKERTLFDLLLAGLTDAQIAVVLGKRSGAIRTAHWRLLIKLRGCLSMLTRLM
                     GGRHAPA"
BASE COUNT         1293 a         2450 c         2510 g         1291 t
ORIGIN      
        1 catttgcctt tactgccacg acccgagtcg tcccggcgcc atcggccgtg ccgtcgtaga
       61 cctcgaggcc gacccggttg ccggtcatga tgtcgccgat ccggaccgga ccagccggcg
      121 tcgagatcag cgcgtcgtac ggctggcagg cgtgaggctg agggtgggtg taggcgtctt
      181 cgctaaggcg gacctcgccg gtggcatcgt caacgtagta gtggccctgg gcggggccgg
      241 tgatgtcgta ggcgtagttc aggccggtgt tgaaccactg cggcgagttg ggagcggcca
      301 tctggttgac gagcatgtag gccagctcgt cgtagaaggc ctgtgcgtcc gcttcactat
      361 tgaaatagcc gtactgctcg ccccagtatc gccagcagcc ggccaggcgg tggatgacct
      421 ggcgggccga gcgctccgga cccgtcttct ggacgccatt ctcgtcgagg accggattgc
      481 cctcgtcgtc gacgagcggc acgccggcct tgcggaaata cttgctgacc atgatgtcgg
      541 ccgcgacctg cgaccacgtc gccggcacct cggcatcggc catctcgaag accaccgtgc
      601 cgtccgggtt cgtgatccgc gaggtccgtt tggaccaact cacactcgaa tagggatcca
      661 gaccaccggc cgtgaacttc cggttgacgg tgaggccgcg gcggcggacc gggcgctgcg
      721 taacgtcggt ctggtattcc tgttcggtca tagtgctgtc ctgattctct gcatgtcgat
      781 caccttgtga gatcgaccac ggctcgccaa tgagcgggat agaatccgga gcgggtgcgc
      841 gatccggaac gggtagcaac tgcgggaaat gttcccggta gtcataaagt caacgaactg
      901 ctaatgtccc cgcggcgaca aatggttcga tcggcgcctg tcgtcgaagc gcctcgctcc
      961 ttgactcccc agccaaactc cgagaactcg tcgccaggaa gggatgacac caccatatct
     1021 agtggtatcg ggcccatcac accgtgggtc ctggacgact agatgtagtg tactcacggt
     1081 taacaacacc acaacatgtg ccgtcatgtc aatgataccc gacgctacag ccgggaaaca
     1141 ggaccgattt gcacaggatt ttcggaccat ttgccaatcc cgcgccgaac acatgttctc
     1201 agcgtacgta cgtattgtca atccccctgc tccgaactgt gccaaccagt cccaaggcat
     1261 ccccggacgg tagcgcatgc atcgccacgg acaggactaa tggcccgttt atggaaagca
     1321 cttgtcgcaa aaagagacgt agcgccagac cccagtggcc ggcatccggt tgacgggatg
     1381 caataccgat ggaaccaggg cagcatcctt gaccgtccca cccaataccg aagcggccca
     1441 gaggcggccg cgcgctacgc taaaggacgc gcctcgatcg ttggttccgc tgcccagcgc
     1501 tgccaggtga agcggaggcg gtagcgctcg agggtgccca ggatcgggcg gccgagggcg
     1561 aggatcagga ccacgttgcc catcgagcgg aagatgtcga accagagcga tgtcacgaca
     1621 taaaaccgca gatagcggcc cagcgcttcg gccaggctca gatccggcga ccatgacagc
     1681 cccggcgaaa cgtcgccacc acccggcgcc gcgaagggcc agaaccagag attgagcagc
     1741 gcgccgaaga tcaacccgct aacagcccca tacccggcca ggatcgcgag ctggcgctcc
     1801 cgccgtgccg gtttcggcag cagaccggcg agcatcccca cccagccgac gccgagcatc
     1861 tgatacggca cccatgggcc gatcccgccg gtcaggaagg cggagacggc cagggtcagc
     1921 gcgccgagct ggaagccgaa ggccggcccg aaaacatacc cgcccaggat caccagcagg
     1981 aagatcggcg atgcgccaag gaacgacggg atcagccgcg ccactgcccc gatcgcgacc
     2041 atcgaggcca gcagcgcgac gatccgggcc gcgccttcgc cgttccgcat cgggtcgatc
     2101 tcgcgcagga tcgcggccag gcaaagcagg atcagcccgc aaaggacaat cggcgcctca
     2161 cccgcccggg tgctgccgga ggcggtctgg tggatcgtgg gcagggcaaa tgggtagagg
     2221 taaacgccca ggccaatcac gctggccagg gcgatcatcc acgtcgaacg aattccggtc
     2281 gactcgattg cttgttcagg ctcgatcatt gtgcaaaact gccaaaaagt cgtgaacagg
     2341 tggttggtga ttggttgatg gtagttggaa taacgttcca tcagccaacc actatccacc
     2401 atccactggg cgccatggct gatatcacca tacaggacct gctggtctgg gagccacgac
     2461 tccagctcgt gcgccgtccg ctgccgggca tgtccgcgaa caacgacctc gaggagcgcg
     2521 agatctcgtg ggcggtcacg atccgggcct cggcgccgat gctccagccc ctgcgcggcg
     2581 gcgagctcgt cctgctgccc gagcggatcg tctcggaatc cgggctggcc atccccgttt
     2641 tgctgcgcga gctgtccgga ttgcatgccg gcgcggcggt cctcgagcgc gcgccggagg
     2701 tcgcctcgcc gatcccggtc ctgatcgcgc ccgaggtcac ggtcgagttc gagactgggc
     2761 tgaaccggat gctgaccgag cggcgcggcg agctctaccg ggccgggaca gatctcggcc
     2821 ggacgttggc cggcatcgcg agcacgcggg acctagcggc gatcatccgc accgccggcg
     2881 agtttctgga ttgccccgtg gccctgatga gcgcgcgcgg gacaatcctc gagcaaacag
     2941 gcaacgcaat gctcccgtcg aatgcggtgc gcgcggtcca atcgatgaac gcgccgcgcg
     3001 aatggcggga tcagcgctat ctggtctccc tggccggcgg aggcgtgctc tggttcgggc
     3061 cggttcccgc cgagcgccgg gccctggtcc ggctggcggc cgaccggatc gcactcgcag
     3121 ccgaaggcgt cctgcgacgg accgtcgagg agcggccgcg aggtccggcc cgctcgtccg
     3181 cgctggccgc cgtgctgcag gggtcggccg aggaggccgc ccgctccggc gcgctgctcg
     3241 atctggcgcc ggatgcgacc taccgggtac tgctcaccgg cgcggagaca gacactggcc
     3301 acctcgaacg ggcgctgcaa tcgctcggca ccgcccattc cgccgggacg atggaagacc
     3361 agactgcggt cgtgatcgag gcgcgggccg agcgggatcg ccgcacgtcg gatgccgcgt
     3421 tggatcgagc gctcggcggc gcgggatgcg actggctggc ggcctcggca tcagtgcggg
     3481 ggatcgggca actaccggag gcgtaccggc aggtgcgcta cctggcggcg ctgatggcga
     3541 gtggggcaat cgcggccggt gccgtgcagt tcgagcagtt ggccgatgtc ggggtctacc
     3601 ggttgctcta cgagctctgg gggacaccgg cgctcgcttc atatattgag gacgcactcg
     3661 gcccgctccg ggccggcgac aagcgcggta tcctgcggga gacgtacctc gcttacctga
     3721 ccgctggcgg atcgcagacc gagaccgccg ccgcgcttgg catccatcgc aacaccctta
     3781 cctatcgcct gcgccagatc gcccagctcc tcggccacga cccggatgat cctcgcctgc
     3841 atctcgccat gcatctcgcg ctggtcgccg agcgcctgcc gggcgagttg agggtggagg
     3901 gaacgtgaaa cgggttgatg gtcgttggtg gttggttgat gggacggaaa accgcccctc
     3961 ccaacgccgc gcggacaggt tgtaattttt cgttccctca accctccacc ctcaaccctt
     4021 aaccacacag ggcgcataat cgcgcggtag cgtggagatg gcagtggcca cgacgacgat
     4081 tccggaatcg atgactcatg ctgaggtgcg gcgggcccag cgggatgcct ggcgcgcaat
     4141 tgccgcgatc ttcctcatca gcggcttcgc actctcctcc tggatctccc gcatcccgac
     4201 cattacggac aaactggacc tcagcaccgg ccatgtcggc acggccctga tggcgatcgc
     4261 cgccggcgcg atcgtcgcct tcccgacgac cggacggttc gtcgacacgc ttggcagcgc
     4321 ccgcacgctg cagatcttcg gtctgatcat gctctgcaca ctgccggtga tcggtctcgc
     4381 gccgaacctg ctgatcctga tcccgatcct gttcgtcttc ggcgcgggca acggcggcct
     4441 ggatgtctcg atgaacgcgc agggcgtcac ggtcgagaag ttcgtcggtc gcaacatcat
     4501 gaactcgctc cacggcttct tcagcatcgg gaccttcgcc ggcgcgatgc tcggagccgg
     4561 cgccgcgacg ctcgatatcc cgcccttcgc gcacttcctg gtcgtgaccg cgatcgggct
     4621 gactgtcctt tggcgggtac gcgattggtt gcagcccgac cctcaggaga cgcaggcgaa
     4681 ggagcccgcg ccggcgtttg cgctgccgcc gcgtccgatc tggctgctcg gcgcgctcgc
     4741 gatctgcgtc tcgatgagcg agggcgcgat ggccgattgg agcggtctct acctgcacga
     4801 ttacctcggc accagcagcg ggttcgccgc gctcgggttc gccgcgttct cggtcttcat
     4861 gctgatcggg cgcttcaccg gcgaccggat ggtggccctg ttcggcgcgg cgcggatggt
     4921 acgctcgggc gcgatcctgg ccgcagtcgg cctcgggatc gcggtgctga tcaacgagcc
     4981 ggtggccatg ctggccggtt tcggggcggt cggactcggg ctgtcggtgg catatccgct
     5041 cgtcttcagc gcggccggca accacccgac actctcggct ggccgggcag tggcgggcgt
     5101 ggcgacgctg ggctatgcgg gtttcctggc cggtccgccc ctgctcggtt ggctggccga
     5161 gccgacctcg ctgcgggtgg tgatggcggt aatcgtctgc ctggccgcgc tgaccgcggt
     5221 cctcgccccg gcagtgcgta ttgccacgaa agatggttga tggtcgttgg tagatagtcg
     5281 ttggatttcc atctaccaac gaccatctac catccaccag gtcacgtagc cgggtgacgc
     5341 tgcgccgcga tcgccggatg ctagaaccgt gcttcggcgc gctggcatcg ctcacgatgc
     5401 cgcgccggta gggaaaggac gaatcatggc cgacaagcca ctcaagaagg cgctcgacct
     5461 ctccgaaaag atcgtgcgcg gggtcgatga gggccaatcg gaagacgccc cgatcgacct
     5521 ccccggcacg caccgggaac gcggcgccga gaatccggag cgggtcgtcg acaagcgcac
     5581 tggtcaggtc gaaaagccgg acaaggaccc cgataacctc atcgaccgca accgctaacg
     5641 cgccggctca ggacacacgg agcggggctg ccgatctgat cggcagcccc gctcctggtt
     5701 gcccggagac ggagattacg gcgctggggt cccgatgtct tgtgacggct tgagcgtgat
     5761 cgcgtagtac gtgaccggct tatcggtaac gttggtgatc gacttgtcgc tgttggtgcc
     5821 gtattgactc aactcggggt cggtgaccag gaaccaggta tcggtgagga gcagtttgct
     5881 ggtggcgccc ggctccacgg tctcccgctg aagatgcagt gtcactgccc cggacagagc
     5941 gggatcaata gcgccgccaa cgcctgagta atcgtatgtc tcggtcacct gccactcgtt
     6001 cggccatgcc gcattgtcgt gcatgaggct caggccgacc ttcacccagt agacaactcg
     6061 cgccggcgtc gactgcgaat tcgttgtctt gtagggtacg tcgctggcga agagtatggt
     6121 gtcgcccgcg cccagcgtcg cgtctgagcc agccgagatc gcttctgccg ttccattggc
     6181 gtcgctgcgg aatacctgga ctggtccgtc agctcgaatt acgagcgatc cgctgaccag
     6241 ggagattagc cggatgccct cacattggca atcgccggtg gcgggcgtcc aggttccgtg
     6301 ggtgttgggc ggaacggtcg ccaggacgaa gaccgatgtg tcctccgcag tcgggccgcc
     6361 gtttggcaat gttcctgccg ggagaacggt atcgaccagc gtcccatcgg tcgcttcgcc
     6421 ggccggtgag ggcgtggccg gggcgaagat cgccggtgct ccggtgtggc tgccggaccc
     6481 accctggtca agcagccaga agccgacgat gccgccgagc gccaccagca ggatggctgc
     6541 cgccacgacg gccgaaagtc ggtgccggac aagctgtggc aggtcgatta tcgggcggac
     6601 tcctcgctgc tcggtcgcga ttcgggaatc gatgagtgac gatgcgacgg gtgagtagat
     6661 cgtgcgtgag acgttcatga ggtgttcctc cagggtgtca acaaaagctg gatcgggaaa
     6721 gtcgtcgtgc tgcgcgaccc gcattcggtc gagcatcgtg accatggccg ggtcgagatc
     6781 tgccgccaac gccgcgaggc gcccggacga ttcgccccga accagggcag tccagtaccg
     6841 gctgactcga ctcggaccgg agcgatcagg caggtgcatg gcgccctccc atcagacgag
     6901 tgagcatgga aagacagccg cgcagcttga tcaggagccg ccagtgggcc gtgcggattg
     6961 cgcccgaccg cttgccgagc acgacggcga tctgggcgtc agtcagcccg gccagcagca
     7021 gatcgaatag ggttcgctct ttctcagtca ggcaacgctc gcgcgcccgg cgaagctcgg
     7081 cggcgtcttc gttgcagatt gcgcggtcct cgggaccgct ctcgggatca gccggatcga
     7141 ggttgccatc cagcgagctg gtctgcaggc cagccgcgcg gtactgctcg ttaacaacgt
     7201 ggtgcgcgat gccgaagagc catccggcga aggcggcatc gttccggcat cggttcagac
     7261 cggcgagcgc ccgggtgaac gtctcctgag tggcctcttc cgccgcctcc cgggtggaaa
     7321 gacggcgcac gctataggca tatacctggt tgacgaactg gcggtagagc agcgcgaagg
     7381 cggcagaatc gccggcacgg gaccgcgcca ccagcattgg catgctgtcg accgcatctc
     7441 ccgccgggat tggctgcgcc acggttgggt tccgcaacat gcgcttcccc tccacgcctc
     7501 gaacgatcca tgtgaacgcg ttcaggaagt aagtgtccgc ggcg
//
LOCUS       DATAOZ010000474        10115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4135544, whole genome shotgun sequence.
ACCESSION   DATAOZ010000474 DATAOZ010000000
VERSION     DATAOZ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10115
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4135544"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VHR64_12140"
     CDS             complement(<1..606)
                     /locus_tag="VHR64_12140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3303625.1"
                     /translation="MDDDITNNMKKSGKEKGKLVGSGLRVALVHDYLTQFGGAEQVLG
                     VLQRVFPDATTFTTLYDPGADLPGIDRDAVEESRLGTVRWFRQHHRAALPLFPLAMRD
                     IRRRLDGFDVVIADSSAWAHQIKPNAGQAFVAYCHSPARFLYGDSDYLGATGVEGPVA
                     HAFHSALAPFRWLDRRAYRRADVVLANSRQVADRLQRRIGID"
     gene            883..2448
                     /gene="rny"
                     /locus_tag="VHR64_12145"
     CDS             883..2448
                     /gene="rny"
                     /locus_tag="VHR64_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX3303626.1"
                     /translation="MDGLLVPVVEALLVAAVSIAGTLLFLERSANSRTRAAQRQADQV
                     LQEAEQVRRDAEISAKDEAIRLRGDLDRELTTRRKEIERIEKRIEQKEENIDRKASSI
                     EQRETAVRRQEQSVTQLKENFEKDRDHKLQEIEELRNRRLGELERIAQLSADEARQEL
                     VAEVESEARAMAARRLHEIEQEINAEAERRSRKVLATTIQRIATDYVAESTVSVVPLP
                     SDEMKGRIIGREGRNIRALEQATGVDLIVDDTPEVITVSAYDPLRRELARRVLQKLLQ
                     DGRIHPTRIEDVVNKTQSELDSTIREEGERVAYEANVVGLHPDLIKLLGRLKFRTSYG
                     QNVLGHSLETSIIAGALAAEIGADINVCKTAGLLHDIGKAVDHEVEGPHALIGADIAR
                     RLGRSAKIVHAIAAHHGEEEPQTVEAFIVATSDAISGSRPGARREMVETYIKRLEALE
                     GVANSFEGVEKSFAIQAGREVRILVSPNKIDDLAASRLARDVSKKIEESLEYPGQIKV
                     TVIRETRAVDYAR"
     gene            3002..3316
                     /locus_tag="VHR64_12150"
     CDS             3002..3316
                     /locus_tag="VHR64_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HEX3303627.1"
                     /translation="MPATTANGYGTAAHLESSSDVLKVSSKSRPSAVAGAIAGVVREI
                     GRAEVQAIGAGATNQAIKAVAIARDYLLETGIDAVCLPAFIDITIDNEDRTAIRLVIE
                     PR"
     gene            3330..4169
                     /locus_tag="VHR64_12155"
     CDS             3330..4169
                     /locus_tag="VHR64_12155"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX3303628.1"
                     /translation="MAQTYADLHTHTTASDGLETPAELVQMASRAGLAAIAIADHDTV
                     DAHLSLTDDEHQALIVIPAIEMSANVGTAEVHILGYFIDPTSQALAESLSQLRRQRLA
                     RVERFCQRLTEIGLPMTMEQVLVHATGESVGRPHIARAMIDQGYVGSVNEAFDRYLAG
                     GRPAFVPRDDVTPEWSIELIHSAGGAAVLAHPLTTDDPAAMMDRLMPAGLDGAEVEYG
                     AYDDTQRTYLRALAESRGLIPTGGSDFHGAAHRENAPLGSGGVPQSIVDWLRERSEGY
                     QRT"
     gene            4274..5323
                     /locus_tag="VHR64_12160"
     CDS             4274..5323
                     /locus_tag="VHR64_12160"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX3303629.1"
                     /translation="MYNLRYLLRAVGFAIGILIGVLSGSILTIGANSQDRKDILVLLA
                     VCIGGLGYIMGPHLKWEAFKRLRDRIAEASIKDILAIAIGLAFGSLVAAPLAFIMSLL
                     PEPAGTVSVIAVATAILGLAITVAIVRRDDLLEPWFKPKTLKVGSQTGRPLVIDTNIA
                     IDGRIIDLMGTGFLPNRLLIPRFVLDELQHIADSDDPQRRTRGRRGLEVLNTMRTDYS
                     SRIEVVDSKVSEERDVDAKLIRLARDRQAALLTNDFNLSKVAQVQGLQVLNLNVLTNA
                     LRPIVNPGQQITLRLVQEGREAGQGVGFLDDGTMVVVDGGKSLVGSEAPVTVTRLLQT
                     GAGRMIFATLGRATA"
     gene            5333..6505
                     /gene="ispD"
                     /locus_tag="VHR64_12165"
     CDS             5333..6505
                     /gene="ispD"
                     /locus_tag="VHR64_12165"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX3303630.1"
                     /translation="MNERIAAVIVAAGQSTRFGAPKILTPIAGIPAILWAVRAFQAVP
                     GWSKLVLVINPEMQPRVEAILAADQSSVRVALVPGGERRQDSVLAGLAAAGDAEVVVI
                     HDGARPLVTPHLIKATIAAVRSGADAAVTAVPVADTLKRDHGERVETVDRSDLWRAQT
                     PQAFRADRLRAALAHADEHGLTVTDEAMAIEMMGGTVTLVPGDEQNIKLTTPGDAVLI
                     EALAGAQSQSRRATIRTGIGYDVHRLVPGRPLILGGIEIPFELGLEGHSDADVLLHAI
                     ADAILGAAAMGDIGRHFPPSDETYRGISSVTLLERSHNIIVAAGYEVVNIDATVIAEE
                     PKIGPHADRMRAVIASALRTDTSAVSLKATTNERLGFAGRREGIAAMAVATLQGRD"
     gene            6510..6980
                     /gene="dtd"
                     /locus_tag="VHR64_12170"
     CDS             6510..6980
                     /gene="dtd"
                     /locus_tag="VHR64_12170"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX3303631.1"
                     /translation="MRILLQRVSSAAVRVDGEMIGEIGRGLVALVGIGHADTPAVVER
                     MAGKCVDLRIFEDDAGKMNRSLRDLGSEPDGAGMLVVSQFTLYADARKGRRPSFTDAA
                     PPILGQELVDTFVSAVRSLAVTVATGQFGAHMVVSLDNDGPVTIWLDSAELSRD"
     gene            7308..7970
                     /locus_tag="VHR64_12175"
     CDS             7308..7970
                     /locus_tag="VHR64_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303632.1"
                     /translation="MDDSPHLQVSAVRQATPTGAPPMLGNSRQAEPLPAESEPKQALP
                     SFMQEGARTHASAPEPVELISANDLPDWIRQIAEADAAKAAAEAAQSAQAAAAAVTDV
                     AATRRPLPGEAPAAAGPSTSWLSKSSIAQESSDPWATTEETAGASWSAADSAAQQQAA
                     YPTISPSWSPSAYEDPGTGKKGRFGRSQRSSSSSEPVYKNRIVQLAFLVLLLALLAAM
                     VL"
     gene            8009..9754
                     /locus_tag="VHR64_12180"
     CDS             8009..9754
                     /locus_tag="VHR64_12180"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEX3303633.1"
                     /translation="MTDTRQDPIDSLVVADFGRGVSRAYLLEAISGGFRFVAKAEHRT
                     TTDLPYEDLSQGWYNLLRQLEWSTGRGLTVRDRLAMPQLESGDGVDGLLISASFGEPI
                     RVAILEAGQSAVASPILDALRRVHTRVFHATAPSSRKDGGWSATQADALRSFQPEMAL
                     LIIGAGAQDAMPRLLQLAKQVGMIGTVSRAIVIADGQAQEQGVAALGNKLKVRSISPV
                     VRAPADIAGEIERELGEAFQIRLRTSDFEVIADEAMQAPISRAHAVDLVNRFIARAFK
                     RQVLTIGVDDGAHAHWASGDQGAISALPQVDLTTAITGLTSREVSDATCWLPFESTED
                     ELVTWALNRSIRPWTIAEQPRDLAIEQALARQVARRAISEVGRMQPMALAGVDLVIGG
                     PVFARWNQPGAAALALLDSIDIVPNNGVVDLALDPDGLMAVAGVVGTIDPALASSLFE
                     YDALTHLGSAIVIGGATSPGDVACRGEIHFDNGEMAQFSVLSGTVEVVPLKSGETATI
                     VLRPERKFSIGGHPAGKTVTLAEERRIVGGTVGVIIDARPRTLASNANRAMQVRQWLE
                     SVNGIRASTAVRKQS"
     gene            9782..>10115
                     /locus_tag="VHR64_12185"
     CDS             9782..>10115
                     /locus_tag="VHR64_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303634.1"
                     /translation="MNQNFPNREPVIVHDQELHVVRRLPSNAEVKVRPGERISGDHVI
                     AKTDPRHLAVRLNIADQLGVSPNDVNKYMLRPVGSTFAAGEAMAKMRKGLRNTVVASP
                     MTGLLLSID"
BASE COUNT         1932 a         3307 c         3149 g         1727 t
ORIGIN      
        1 atcgatgcca atccgccgct ggaggcgatc ggcaacctgc cggctattgg ccaggaccac
       61 gtcggcccgt cggtacgcgc ggcggtccaa ccagcgaaat ggcgcgagcg ccgagtgaaa
      121 cgcatgcgca actgggccct cgaccccggt cgcaccgagg tagtcagaat ccccatacag
      181 aaatcgagcc gggctgtggc aataggcaac gaaggcttgg ccggcattcg gcttgatctg
      241 gtgggcccat gcgctggagt cggcgatgac cacatcgaag ccgtccagtc gccgccggat
      301 gtcccgcatc gcgagcggaa aaagcggcag cgctgcgcgg tgatgctgcc ggaaccaacg
      361 aacggttccc agccggctct cctcgaccgc atcacgatca attccaggga gatccgctcc
      421 gggatcgtag agcgtcgtga aggtcgtggc gtccggaaag actcgctgca gcacacccag
      481 gacctgttcg gcgccgccaa actgggtgag gtagtcatgc accaaggcaa cgcgaagtcc
      541 cgacccgacc aatttcccct tttccttgcc cgacttcttc atattgttcg ttatgtcatc
      601 atccaccaag atgccagcgg ccgataatgt actttcatca acatatcttg gccatattaa
      661 ccgaaaccat gaacactttc gatgtaataa gccacaaatg acgatgcgta gatttccatc
      721 gggtttgaca tcattttcga gaaaaccgta tgatcaacag aacatcgcgg cactagacga
      781 ttgtcgagac cccgatatta gacactgact gtttcgcata ccgcgaaacc gaaccaattg
      841 gaaccgcgcc actacatcac ggcgccccag gagagtatga ccgtggatgg acttcttgtc
      901 cccgtggtcg aagcgctcct cgtcgcagcg gtctcaattg caggtaccct gcttttcctg
      961 gagcgcagcg ccaactcgcg tacgcgagct gcccagcgcc aggctgatca ggtcctgcaa
     1021 gaggccgaac aagttcgacg agacgccgag atctcggcca aagatgaggc aattcgcctt
     1081 cgaggcgatc tggaccggga actaaccaca cgccgcaaag aaatcgagcg tatcgagaag
     1141 cggatcgagc aaaaagaaga gaacatcgat cgcaaggcca gctcgatcga gcagcgcgag
     1201 acggcagtcc gccggcaaga acaatcggtc acccagctca aggaaaactt cgagaaggac
     1261 cgtgatcaca agctccagga gatcgaagaa ctccgcaacc ggcggcttgg cgagctggag
     1321 cgaatcgcgc agctttcggc ggatgaggcg cgccaggagc tggtggccga ggtcgaatcc
     1381 gaggcccggg ccatggcggc ccgccgcttg catgagattg agcaagagat caatgccgag
     1441 gccgagcgcc gctcgcgcaa agttctcgcc accacaatcc agcggatcgc gaccgattac
     1501 gtcgcggaat ccaccgtttc cgtggtgccg ttgccaagcg acgagatgaa ggggcggatc
     1561 atcggccgcg agggccgcaa tatccgcgct ctcgagcagg ccaccggcgt cgacctgatc
     1621 gtcgacgaca cgccggaagt catcaccgtc tctgcgtacg atccgctccg ccgcgagctg
     1681 gccaggcgcg tgctccagaa gctactccag gacggccgca tccatccgac ccggatcgag
     1741 gacgttgtca acaagacgca gtccgagctc gacagcacga tccgcgagga aggcgagcgc
     1801 gtcgcctacg aggcaaacgt ggtcggcctt catccggacc tgatcaagct gctgggccgg
     1861 ctcaagttcc gcaccagcta cggccagaac gtgctcggcc actcgctcga gacgtcgatc
     1921 atcgccggcg cattagccgc cgagatcggc gcagacatca acgtctgcaa gacggcagga
     1981 ctgctccatg acatcggcaa ggcggtcgac catgaggtcg aaggtccgca cgccctgatc
     2041 ggcgcggata tcgcccgccg tctcggtcgc tcggccaaga tcgtccatgc gatcgcggcg
     2101 caccacggcg aagaagagcc acagactgtc gaggccttca tcgtggccac ctcggacgcg
     2161 atcagcggct ctcgcccagg cgcgcgccgc gagatggtcg agacctacat caagcgtctc
     2221 gaagcgctcg agggtgtggc gaactccttc gagggtgtcg agaaatcctt cgccatccag
     2281 gccggccgcg aggttcggat cctggtctcg ccgaacaaga tcgacgacct tgcggcaagt
     2341 cgcctcgcgc gcgatgtcag caagaagatc gaggagtcgc tggagtaccc gggccaaatc
     2401 aaggtcacgg tgatccgcga aacccgcgca gtcgactacg ctcggtaaca ttgaccactc
     2461 agggccattc atgtagcctt cggtgagtgc atttctgaac actcgccggg gagtgcccga
     2521 gttttcatcg cctaccgatc gccgagcctg aacatcgccg tagatagttc aggctcggtg
     2581 cccatgacaa cggagtcctc gatcgggacg caggttccag ggtcaacttc aacggagtga
     2641 tcagcgcgtc acgttgatcg cgagtgagct gcaccaggtc ggctgccata ccgacttgtg
     2701 cctgtcacgg atcgctgacc gttgggtggt ggttcgcaaa gaacgacgcc tgaccttgcg
     2761 caacggacct tgcgcatccg cctggagcac attgatggat cgatttttca gcaagctcga
     2821 cctgcgcgat attaagattg accccgagcg ttcgcgcttc tcgcccgacg acgaattcga
     2881 gatcgacggc gagtcggacg gagctgcgca cgggtcggcg caggttacat acgagactac
     2941 cccgcccgcc actgagccgt ctgacgactg gccagtcaca gaggcagcgg aggaagtcga
     3001 aatgccggcc acgaccgcca acggctacgg cacggcggcg cacctcgaaa gctccagcga
     3061 cgtcctgaag gtgtcatcga agtctcgtcc aagcgcggtc gccggcgcga ttgcgggtgt
     3121 tgttcgcgag atcggccggg ccgaggttca ggctatcggc gccggcgcca ctaaccaggc
     3181 aatcaaggcc gtcgccatcg cgcgggacta tctgctcgag acgggaatcg acgccgtttg
     3241 cctgcccgcc ttcatcgaca tcacgatcga caacgaggat cggactgcca tccgcctggt
     3301 tatcgagccg cgctaggcat ctcgcccgcg tggcacagac ctacgccgat ctccacaccc
     3361 acacgaccgc atcagacggc ctggagacgc cggcagagct cgtccaaatg gcgtcccggg
     3421 ccggattggc cgcgatcgcg atcgctgatc acgacacggt cgatgcgcac ctgtcgttga
     3481 cggacgacga acatcaggcg cttatcgtta tcccggcaat cgagatgagc gcgaacgtgg
     3541 gtaccgccga ggtccacatc ctcggctact tcatcgatcc gacaagtcag gcattggccg
     3601 agtcgctctc ccaattgcgc cgccaacggc ttgcgcgcgt cgaacgattc tgccagcgcc
     3661 tgaccgagat cggccttccc atgaccatgg agcaggtcct ggtccacgca accggcgaat
     3721 cggtcgggcg gccgcacatc gcgcgcgcca tgatcgatca ggggtacgtc ggctccgtca
     3781 atgaggcgtt cgaccgctac ctcgcgggcg gccgaccggc attcgtaccg cgggacgatg
     3841 tcacgccgga atggtctatc gagctgatcc actccgccgg tggcgcagcc gtgctcgcgc
     3901 atccactgac cacggacgat ccagccgcga tgatggatcg cctgatgcct gccggactcg
     3961 atggcgccga agtcgaatac ggcgcgtacg acgacaccca gcgcacctat ttgagagccc
     4021 tggccgagtc ccgcgggctc atcccgacgg gagggagcga ctttcacggc gccgcgcatc
     4081 gcgaaaatgc accccttggt agtggcggag tgccacagtc gatcgtcgat tggctccggg
     4141 agcgatccga agggtaccag cgaacctagc cttttttccc actactggtg tttccgctca
     4201 ggcacatggc tggtagggtt ggcagtggtc ggattcggcc aatgcacttt tctcttgagc
     4261 ggatattggg ctcatgtaca acctcagata cctgttgcgt gccgttggct ttgccattgg
     4321 catcctgatt ggtgttctct ccggatcgat cctgactatc ggtgccaaca gtcaggatcg
     4381 gaaagacatc cttgtcctct tggccgtctg tatcggtggc ctcgggtaca tcatggggcc
     4441 gcacctcaaa tgggaagcgt tcaagcgcct gcgggaccgg attgccgagg catcgatcaa
     4501 ggacatcctc gctatcgcga tcggattggc gtttggctcg ctcgttgccg cgccgctggc
     4561 cttcatcatg tctctcctcc ccgagccggc tggcacggtc tccgtgattg ccgtagcgac
     4621 cgccatcctt gggctcgcaa tcaccgtggc gatcgtgcga cgcgatgacc tgctcgagcc
     4681 atggttcaag cccaagaccc tgaaggtcgg cagtcagaca ggacgaccgc tggtcatcga
     4741 cacgaacatc gccatcgatg ggcgcatcat cgacttgatg ggcaccggct tcctgccgaa
     4801 tcgcctgctt attccgcgct tcgttctcga cgagctgcaa cacatcgccg attccgatga
     4861 tccgcaacgc cgcacgcgcg ggcgccgggg tctcgaagtc ctgaacacca tgcgtaccga
     4921 ttacagctcg cgcatcgagg tggtcgactc caaagtctcg gaggagcgtg atgtcgacgc
     4981 gaagttgatc cggctggcca gggatcgcca ggccgccctg ctgaccaacg acttcaacct
     5041 gagcaaggtg gcacaagtgc aaggcctgca ggtgctgaac ctgaacgtgc tcacgaatgc
     5101 cctgcgtccg atcgtcaacc cgggacagca gatcacgctc agactggtcc aggaagggcg
     5161 ggaagccggg caaggtgtgg gcttcctcga cgacggcacg atggtcgtgg tggatggcgg
     5221 caagtcgctg gtcgggtccg aagcaccggt cacggtgacg cgactcctgc aaacaggagc
     5281 gggccgcatg atcttcgcca cgctgggcag agccaccgcg taaacttccg ccgtgaacga
     5341 acgaattgcc gccgtcatcg tcgcggccgg ccaatcgacc cgcttcggcg cacccaaaat
     5401 cctgactccg attgcaggca tcccggccat tctgtgggcc gtgcgcgcat tccaggcggt
     5461 tcccggctgg tcgaagcttg tgctcgtcat caacccggag atgcagccca gggtcgaggc
     5521 gatacttgcc gccgatcaat cgtcggtcag agtcgcgctt gtccccggcg gtgagcgacg
     5581 tcaggactcg gtcctcgccg gcctcgcggc ggcgggagac gccgaagtcg tcgtcatcca
     5641 cgacggcgcc cgacctctgg tcactccaca cctgatcaaa gcgaccatcg ccgccgtccg
     5701 ctccggggcc gacgcagcgg taacggcggt gcccgtagcg gatacgctca agcgggacca
     5761 tggcgaacgg gtcgagacgg tcgaccggtc agatctctgg cgagcgcaga caccacaggc
     5821 attccgcgcg gatcgcttgc gggctgctct cgcccatgcc gacgagcacg gactgaccgt
     5881 tacggacgag gcaatggcga tcgagatgat gggaggaacc gtgacgctgg tgcctgggga
     5941 cgagcagaac atcaagttga cgacgcccgg cgacgccgtc ctgatcgaag ccctggccgg
     6001 cgcgcaaagc caatcacgac gagcgaccat acgcaccggg atcggctacg acgtgcatcg
     6061 actggttcca ggccgtccgc tgatcctcgg cggcatcgag attccgttcg agctcggtct
     6121 ggaggggcac tcggacgccg atgtcctgct ccacgccatc gccgacgcca tcctcggtgc
     6181 ggccgcaatg ggcgacatcg gtcggcactt tccgccctcc gacgagacct atcgcggcat
     6241 cagcagtgtc acgttgctgg agcgcagcca caacatcatc gtcgcggccg gatatgaagt
     6301 ggtcaatatc gacgctaccg tcatcgccga ggagcccaag atcgggccac acgccgatcg
     6361 aatgcgcgcc gtcatcgcat ccgcgctgcg gactgatacc agtgcggtca gtctcaaggc
     6421 aaccaccaac gagcgcctcg gctttgccgg ccggcgcgaa gggatcgctg cgatggcggt
     6481 cgccacgctc caggggcggg actgaatcga tgcgtattct gctccagcgg gtcagctcgg
     6541 ccgcagtacg cgtcgatggc gagatgatcg gggagattgg ccgcggtctt gtcgccctgg
     6601 tgggcatcgg tcatgcggac acaccggcgg tcgtagagcg gatggccggc aaatgtgtcg
     6661 atctccgcat cttcgaggat gacgccggca agatgaatcg ctcgctgcga gacctcggca
     6721 gcgagccaga tggagccggg atgctcgtcg tctcgcagtt cacgctatac gcggacgcgc
     6781 gcaagggccg tcgcccaagt ttcaccgatg ccgcgccgcc gatcctcgga caagagttgg
     6841 tcgatacctt cgtgtcggcc gtacgatcgc tggccgtgac agtcgcaacc gggcaattcg
     6901 gcgcgcacat ggtcgtcagc ctcgacaacg acgggccggt cacgatctgg ctcgacagcg
     6961 cggaattgtc gcgcgactga tcgctcgcat cggtccagcc acatcgggac ttttcggtgt
     7021 ctccgcctga ggagtcaccc ggaccccctc cctatactcg aatctcagtg cgacatgctc
     7081 ggcataccga tgcttcggcg ctgccggaca gttcggcagc acggcgaacg aggccggtgg
     7141 agggacgacc cttgattatt tgcgagatgt gcggtgccgc caatggcgag ctcgagactg
     7201 agtgccgcat ttgcggacat tcgctggcaa cccaaacgga accggtagcg gcaactgttg
     7261 ctccaccgca acacgtcgcg gcacccgcag cggagcggca agcaccggtg gacgattcgc
     7321 cccacctgca ggtgtcggcg gtacggcagg caaccccgac tggcgcaccg ccgatgctcg
     7381 ggaattcgcg acaggcggag cccctcccag ccgagtctga accgaagcag gctttgccgt
     7441 ccttcatgca ggaaggcgcg cgcacccatg catcggcgcc tgaaccggtc gagctgatct
     7501 cggccaacga tcttcccgac tggattcgcc agatcgccga ggccgatgcc gcaaaggccg
     7561 ccgctgaagc cgcgcagtcc gctcaggcag ccgcagccgc cgtgaccgac gtcgcagcaa
     7621 ccaggcgccc gcttccgggc gaggcccctg ctgccgctgg cccgagcacc tcttggctga
     7681 gcaagtcgtc gatcgcgcag gaatccagcg acccctgggc aacgaccgag gagacagccg
     7741 gggcgtcctg gagcgcggcc gattccgccg ctcaacagca ggctgcctat ccgacgatca
     7801 gtccgtcatg gagcccatcc gcctacgaag atccgggcac cggcaagaag gggcggttcg
     7861 gccggtcgca gcgctccagc tcgtccagtg agccggtcta caagaaccgg atcgtgcagc
     7921 tcgcattcct cgttctgctg ctggcgctgc tggcggcaat ggttctctag gcacgcgcac
     7981 acgcggccgc tggtacggag tctctcgcat gaccgacact cgtcaagatc ccatcgactc
     8041 gctcgtcgtt gccgacttcg gtcggggcgt ctcccgcgcg tacctgctcg aagcgattag
     8101 cggcgggttc cgattcgtcg ccaaggccga gcaccggacg accaccgacc tgccatacga
     8161 ggacctctcg cagggttggt acaacttgct gcgtcagctc gaatggtcga ccggccgagg
     8221 cctgaccgtt cgtgacaggc tggccatgcc gcagctcgag agcggggatg gcgtcgacgg
     8281 attgctgatc tccgcctcct ttggcgagcc gatccgggtc gcgatcctgg aggccggcca
     8341 gagcgcggtc gcgtccccaa tcctggacgc cctgcgacga gtccacaccc gggtcttcca
     8401 cgccaccgcg ccctcgtcgc gcaaggatgg cggctggtct gcaacgcagg cggatgcact
     8461 tcgctcgttc cagccagaga tggccctgtt gattatcggt gccggcgcgc aggatgcgat
     8521 gccgcggctg cttcagctcg ccaagcaggt tgggatgatc ggcacggtct cgcgcgctat
     8581 cgtaatcgcc gatggacaag cccaggagca gggtgtcgcc gcgcttggca acaagctcaa
     8641 ggtgcgctcg atctcgccgg tcgtacgcgc acccgccgat atcgccggag aaatcgagcg
     8701 cgagttgggt gaggcattcc agattcgtct ccgcacttcg gatttcgagg tgatcgccga
     8761 cgaagctatg caggcgccga tctcgcgagc gcacgcggtc gacctcgtca atcgatttat
     8821 cgcccgcgct ttcaagcgac aggtcctcac tatcggcgtc gacgacggtg cgcacgcgca
     8881 ttgggcgagc ggcgaccagg gcgccatctc ggcactgccc caggttgacc tgacgaccgc
     8941 gatcaccggt ctgaccagcc gcgaagtctc cgatgcaacc tgctggctgc cattcgagag
     9001 caccgaagat gaactggtca cctgggccct gaaccggtct attcggccgt ggacgattgc
     9061 cgagcagccg cgcgatctcg cgatcgagca ggcgctcgcc cgtcaggtcg cccgccgggc
     9121 gatctccgag gtgggtcgca tgcagcctat ggcgctggcc ggagtcgatc tggtcatcgg
     9181 cggtccggtc ttcgcgcgct ggaatcagcc cggagcggca gcgctggcac tgctcgactc
     9241 catcgacatc gtgccgaaca acggcgtcgt cgaccttgcc ctcgatccgg acggtctgat
     9301 ggcggtcgcc ggcgtagtcg ggacgataga tcccgcgctc gcctcgagtc tcttcgagta
     9361 cgacgcgttg acgcatctcg gcagtgcgat tgtcattggc ggcgcaacga gcccgggcga
     9421 tgtcgcctgc cgtggcgaga tccacttcga caacggcgag atggcgcagt tcagtgttct
     9481 gtccggcacg gtggaagtgg tcccgctcaa gagcggcgag acggccacga tcgtactgag
     9541 gcccgagcgc aagttctcaa tcggcggtca cccggccggc aagaccgtga cactcgccga
     9601 ggagcgtcgc atcgtcggcg gcactgtggg cgtgatcatc gacgctcggc cgcgcaccct
     9661 tgcgagcaac gcaaatcgag cgatgcaggt tcgccagtgg ctcgaatccg tcaacggcat
     9721 ccgcgcgtcg accgccgtgc gcaagcagtc ctaaaccggc cctgtacgaa ggacaaggat
     9781 catgaaccag aacttcccga accgcgagcc cgtgatcgtc cacgaccagg agctgcacgt
     9841 tgtccgccgg ctaccgagca atgccgaggt caaggtccga cccggtgagc gcatcagcgg
     9901 tgatcacgtg atcgccaaga ctgatccgcg ccacctggct gtccggctca acatcgccga
     9961 ccagctcggc gtctcaccga acgatgtcaa caagtacatg cttcgaccgg tcgggtcgac
    10021 ctttgccgcc ggcgaagcga tggcgaagat gcgcaagggt ctccgcaaca ccgtcgtggc
    10081 ctctcccatg accggcttgc tcctctcgat cgatt
//
LOCUS       DATAOZ010000475         3815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_196341, whole genome shotgun sequence.
ACCESSION   DATAOZ010000475 DATAOZ010000000
VERSION     DATAOZ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196341"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /gene="rlmN"
                     /locus_tag="VHR64_12190"
     CDS             <1..803
                     /gene="rlmN"
                     /locus_tag="VHR64_12190"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX3303635.1"
                     /translation="GDLVETVLMLYRSRATVCVSCQVGCAVGCAFCATGLMGLDRNLT
                     TGQMVAQAVGAARTAREMGRELTNLVMMGMGEPFHNYPATIKFLALLNDPNGFGMGAR
                     RMTVSTSGIVPFIDKLADEPLQVNLAISIHAPNDELRSSLVPINRKHPVTDLMAAVDR
                     YTAQTHRRVSFEYALMSGINDSDEIALELAELMRGRLCHLNVIPFNKVDVLEFERPTA
                     DGIERFAELAGSLGTPVTVRYSRGLDISAACGQLRAKQLNEEPVEHSI"
     gene            complement(818..1153)
                     /locus_tag="VHR64_12195"
     CDS             complement(818..1153)
                     /locus_tag="VHR64_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3303636.1"
                     /translation="MEFFVGIPVRSLEGSLDWYKRLLGREPDLLDGDHAAVWDLAEGR
                     SLYIVDNPEHVGHLVFTMYFDDLDTVIADIAERGIEPVDQSTNEGDVRTFRFRDPDGN
                     EIDFGWYPH"
     gene            complement(1240..2250)
                     /gene="speB"
                     /locus_tag="VHR64_12200"
     CDS             complement(1240..2250)
                     /gene="speB"
                     /locus_tag="VHR64_12200"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX3303637.1"
                     /translation="MRPFRPGESGYVGPGITFLRTPLVLDPEELTDVDVAVLGAPFDE
                     GVTYRPGARFGPRAIRLADHSGIGGRTHMEVGVDPKAVLKIVDFGDIEAPPSDLLKAH
                     ALLRSHVGMILNKGAVPVVLGGDHSLSAPMMQAIADCYGPDGYAVIHFDTHADTGFLD
                     EPTPYNHGTPFYRSVTEGFMRGDHIFQIGLRGTWPSPPEFDWMREVGFRWRTMDEVME
                     LGLNAVVDEAIAYATSKAPRVYLTVDIDVLDPAFAPGTGTPEPGGLMTRELLSAVRRI
                     SSSVDLCAMDMVEVSPPYDVAEITAQAAHRVVLETLTGIALRRSGQSACPQRSRVVDF
                     AR"
     gene            complement(2481..>3815)
                     /locus_tag="VHR64_12205"
     CDS             complement(2481..>3815)
                     /locus_tag="VHR64_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3303638.1"
                     /translation="TDLEERRLNANTEVSAQMYERAKTHMVGGVPSSYQSRDPWPIYL
                     TRGKGSKVWDVDDTEYIDFHHGFGSMVQGHAHPAIVKAVQERVELGTHFAAPMEDDII
                     VSDELAKRFRLKDWRYVNSGSEATMDAIRIARALTGRDTIMKIFGSYHGHHDYVMVSI
                     GVPYDKIGDRDNYASLPYGAGIPQAVVDMTIAVPYNDAGALERRIERLIEEGRKPACL
                     IMEAALMNLGVVLPQPGYLEAVREITKKHGIVWIVDEVKTGLCVAPGGATDKFNLSPD
                     MVTLAKALGGGLPSGAIGGTSEVMDIVRSGKVYQVGTYNGNPLTMAAARASLLEVLTP
                     EAYDHLNTLNDHLLAGCDAVLKKYDLPGYTVGISSKGCVTFSPVEITDYESFKANQDG
                     EITELAWLYAMNRGIYMTPGREEEWTLSVQHSIEDCDQYIAMFEELCHDLTN"
BASE COUNT          688 a         1198 c         1250 g          679 t
ORIGIN      
        1 agggcgacct cgtcgagacg gtcctgatgc tctatcgctc ccgggcgaca gtctgcgtct
       61 cctgccaggt cggctgcgcg gtcggctgtg cgttttgcgc gaccgggctg atgggcctgg
      121 accgcaacct gacgaccggc cagatggtcg cgcaggccgt tggtgcggct cgcacggcgc
      181 gggagatggg gcgggagctg accaacctcg tgatgatggg catgggcgag ccgttccaca
      241 actacccggc cacgatcaag ttcctggcgc tgctcaacga cccgaacggg ttcggcatgg
      301 gggcgcgccg gatgacggtt tcgacctcgg gcattgtccc gttcatcgac aagctggccg
      361 acgagccatt gcaggtcaat ctggcgatct cgatccacgc gccgaacgac gagctgcgct
      421 ccagcctggt gccgatcaac cgcaagcacc cggttaccga tctgatggct gcggtcgatc
      481 gctacacggc gcagacgcac cggcgcgttt cgttcgagta cgcgctgatg agcggcatca
      541 acgattcgga cgagatcgcg ctggagctgg ccgagctgat gcgcggccgg ctctgccacc
      601 tgaacgtgat cccgttcaac aaggtcgacg tgctcgagtt cgagcggccg accgctgacg
      661 gcattgagcg attcgcggaa ctggccggct cgttaggcac gccggtgacc gtgcgctact
      721 ctcgtggcct ggatatctcc gccgcctgcg ggcagttgcg ggccaagcaa ctcaacgaag
      781 agccagtcga gcattcaatc tagcagcgaa cgcgctgtta atgcgggtac cagccgaagt
      841 cgatttcgtt gccgtcggga tcccgaaacc ggaaagtgcg gacatccccc tcgttcgtcg
      901 attgatcgac tggttcgatc ccgcgctcgg cgatgtcggc tatgacggtg tccagatcgt
      961 cgaaatacat cgtgaacacg agatgaccga cgtgttcggg gttgtccacg atataaagcg
     1021 atcgaccctc agccagatcc cagacggcag catgatcgcc atcgagaagg tcgggttccc
     1081 gacccagcag acgtttatac caatcgagcg acccttcgag agaccgaact ggaataccga
     1141 caaagaactc catatgcgtc aatctgccct tgcgcgaccc tacaatccgg cgagtgcgag
     1201 cggcgattgc cgctcactcc agcatctatt cgtcgtttgt caacgagcga aatcgacaac
     1261 ccgcgagcgc tgcgggcacg cgctttggcc ggagcggcgg agggcgatgc cggtcagggt
     1321 ttcgaggacg acacggtgcg cggcctgggc ggtgatctcg gccacatcgt agggcggcga
     1381 gacctcgacc atgtccatcg cgcagagatc gacgcttgac gagattcggc ggaccgcgct
     1441 caacagctcc cgcgtcatca gaccgcccgg ctccggcgtt ccggtcccgg gcgcgaaggc
     1501 tggatcgagc acgtcgatgt cgacggtcag gtagacgcgc ggggccttgc tcgtggcgta
     1561 agcaatcgcc tcgtcgacca ccgcattcag tcccagctcc atcacctcgt ccatcgtccg
     1621 ccagcggaag ccgacctcgc gcatccagtc gaactcgggc gggctcggcc acgtgccgcg
     1681 caggccgatc tggaagatgt ggtcgccgcg catgaagccc tcggtcacgc tgcggtagaa
     1741 gggtgtgccg tggttatagg gcgtcggctc gtcgagaaag ccggtgtcgg cgtgcgtatc
     1801 gaagtggatg accgcgtagc cgtcgggacc gtagcaatcg gcgatcgcct gcatcatcgg
     1861 cgcggagagc gagtgatcac cgccgagtac gaccggaact gcgcccttgt tgaggatcat
     1921 cccaacgtgc gagcggagca gggcgtgggc cttgagcagg tcgcttggcg gggcctcgat
     1981 gtcgccgaag tcgacgatct tgagtacggc cttgggatcg accccgacct ccatgtgcgt
     2041 ccgcccgcca atccccgagt ggtccgccag gcgaatcgcg cgcggtccga agcgggcgcc
     2101 gggccgatag gtcacgcctt catcgaaggg cgcgccgagg accgcgacat cgacatcggt
     2161 caactcttcg ggatcgagga cgagcggagt tcgcaggaag gtgatgccag gaccgacata
     2221 tccggactcg ccgggacgaa atggacgcat tcaggttcct cgcacgccaa aggaccgcgg
     2281 gtgcaccacg ccggacacca tcagattggt cgcttgcgga tggaatggta attgatgagg
     2341 cgagcagtcg aggaggcgag cagtcggcaa acggaaaacc tgagaccaga agggacggtc
     2401 caagagaacc gccccttcta tccactaatc accaaccacc aaccactatt cactgacgtc
     2461 cgaagtccga cgtctcgcaa ctagttcgtc aggtcgtggc aaagctcctc gaacatcgcg
     2521 atgtactggt cgcaatcctc gatcgagtgc tggacggaga gggtccactc ctcttcgcgg
     2581 ccaggcgtca tgtagatgcc ccggttcatc gcgtagagcc aggccagctc ggtgatctcg
     2641 ccgtcctggt tggccttgaa cgactcgtag tccgtgatct cgaccggcga gaaggtgacg
     2701 cagcccttcg agctgatccc gacggtgtac cccggcaggt cgtacttctt gaggacggca
     2761 tcgcagccgg ccagcagatg atcgttgagg gtgttgaggt gatcgtaggc ctccggagta
     2821 agaacctcga gcaggctggc ccgggctgcg gccatggtca gcgggttccc gttgtaggtg
     2881 ccgacctggt agaccttgcc ggagcggacg atgtccatca cctcggacgt tccgccgatc
     2941 gcgccggagg gcagcccgcc gccgagcgcc ttggccagcg tgaccatgtc cggcgagagg
     3001 ttgaacttgt cggtcgcgcc gccgggagcg acgcagaggc cggtcttgac ctcgtcgacg
     3061 atccagacga tgccgtgctt cttcgtaatc tcgcggaccg cctcgaggta tccaggctgc
     3121 gggaggacga cgccgaggtt catcagcgcc gcttccatga tcagacaagc cggcttgcgc
     3181 ccttcctcga tcagccgctc gatgcgccgc tccagcgcgc cggcgtcgtt gtacggcacg
     3241 gcaatcgtca tgtcgacgac tgcctgcggg atgcccgcgc catacggcag ggaggcgtag
     3301 ttgtcgcgat cgccgatttt gtcgtacggc acgccgatcg agaccatcac gtagtcgtgg
     3361 tggccgtggt acgagccgaa gatcttcatg atcgtgtcac ggccggtgag tgcgcgggcg
     3421 atccgaatcg cgtccatcgt cgcttccgaa cccgagttga cgtaacgcca atccttcagc
     3481 cggaagcgct tggccagctc gtcggagacg atgatgtcgt cctccatcgg cgcggcgaaa
     3541 tgcgtgccga gctcgacgcg ctcctgcacg gccttgacga tggccgggtg cgcgtggccc
     3601 tggaccatcg agccgaaacc gtggtggaag tcgatgtact cggtatcgtc gacgtcccag
     3661 accttcgagc ccttgccgcg cgtgaggtag atcggccacg ggtcgcgcga ctggtaggag
     3721 gacggcacgc cgccgaccat gtgcgttttc gcccgctcgt acatctgggc cgagacctcg
     3781 gtgttggcat tgagccgccg ctcttcgagg tcggt
//
LOCUS       DATAOZ010000476         4791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3611118, whole genome shotgun sequence.
ACCESSION   DATAOZ010000476 DATAOZ010000000
VERSION     DATAOZ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3611118"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VHR64_12210"
     CDS             <1..288
                     /locus_tag="VHR64_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3303639.1"
                     /translation="KFVATGITKHLAIEWADRGIRVNAVAPGTINTERVQRLPEEPGG
                     PEYLEAIKKLHPMGRIGDPAEVANAILFLASDEASFITGVVLPVDGGYLAQ"
     gene            525..3089
                     /locus_tag="VHR64_12215"
     CDS             525..3089
                     /locus_tag="VHR64_12215"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX3303640.1"
                     /translation="MSETAQSIASQDTVTLPGALPGVTPGDARARVRELVDRAWFPDT
                     LGVIGVLLVTALLAWHRLWLENGLAYLDVATFYMPWYAHLGEAVRALDIPGWNPYVFS
                     GTPFAGDPQSGWWYFPAMAIFSLFEPVRAYQIFLIFHLAFAGVTTYILCRMYGLQVIP
                     SLAAGAAYQSGPFVSHVSCCLIHVQLATWIPMALIGVETTVRSKHTNRRAIGWIITAF
                     AMSQMIAGWPGQGMYNGCLLTGAYLAFRILLSRENGVLGWRDRIVRLIGDSAGVLSFA
                     LLWAAAGILPRIDIVSRTNVAWGEYDGFETNSYASGWQIYTVLDRLFSDNNGYRSLLF
                     YLGAPIIILMFIGIVLAWQRPWVKFMTGVTILTSIMTLKPTFIHEIVFLLPRYEALHN
                     HVPSRILAVQWIGPVVLAATALDTLMRSPSRDRILRAVGIAFGIWTGVALLLQVGNRP
                     VPGAVIVAAVMTAGVVCLFAIITTPPSDRYSRFVPRAQAGLAILLMLLVIADPAGRRL
                     VSTLETGQTMSEVIRVPTGPISRQAVPDNDASTDPGGAGEFLQAKRDEGEYFRYFGYD
                     NVLQQGGEGYPSTYRENFGNPTAISILVNARAMRLHLNDVQGYNPVQLKNYVTYLNEL
                     NGAVQNYHDAQILPGGLNSPLLNILNARYIVIPNEAIGQRPRADIMLLLASYPEVFRS
                     DTVRILENPHAMPRVWIAHNVVQAQPELAAAMIEAPGFDPAETVILSPVAGPVAATQP
                     ANPKAESVQIVTYEPDRVVLKVHAESDGVLVLSETYEEGWHAHVDGDKAPVDEAYGVV
                     RAVPVTAGDHTVVLTYDPWSLRYGFYLSLLGAALSLAVIGAYAVRRMRGGALEVDG"
     gene            3089..3802
                     /locus_tag="VHR64_12220"
     CDS             3089..3802
                     /locus_tag="VHR64_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303641.1"
                     /translation="MSDEQGPVRPRANRTPRRREPRRLVASPHRSGRTLAILAALVIA
                     SIFISYDVAGIADLQKDPVTGVDYVPVVNYVQARRQPGEKVLVALPPPAYLAFKSTDD
                     LIFLSSPISRKRAQRYTRLTEDGGYVEYWTGVDSVVDTASLCQTLYNEPGLWLIVDEA
                     RLNADWAFQGSMATVIRGLTYVRYEAEGGAMVRRLAPLPSRDPVAEQLCAEAQTGKVT
                     DSDEIVDFSQPEPTPTPEP"
     gene            complement(3931..>4791)
                     /locus_tag="VHR64_12225"
     CDS             complement(3931..>4791)
                     /locus_tag="VHR64_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303642.1"
                     /translation="QRWNDASADLATVSTLNYPVVTFSYGAVADTLVEAYEPGLLDTM
                     RLITVRDGELEKTLSNDTIPQKGITIADVDAGKLDELLPQTPDNDVIWYLYYQRRGEE
                     FVRAGIENAGYVRVIDKVYDAPRGLVFLDLYARPGANLGDVVPGMQPFSNETAWGIPT
                     GLSPVEPTEDGTAVHITNQSRTGTAVVTQIKTGGAALYTFDVDVQTRLTGGRALVTLT
                     CQTQAGGVLSELTAGTSGERVNDVMHHQSAIFCPADTDQVRITLRNLGNGEMTFINPS
                     LRKIQIPPRP"
BASE COUNT          768 a         1555 c         1560 g          908 t
ORIGIN      
        1 aagttcgtcg ccaccggcat caccaagcac ctcgcgatcg agtgggccga tcggggcatc
       61 cgggtaaacg cggtcgcgcc ggggacgatc aataccgagc gagtgcagcg cttgcccgaa
      121 gaaccaggcg gaccggagta cctcgaggcg atcaagaagt tgcatccgat gggtcggatc
      181 ggcgatccag ccgaggtcgc caatgcgatc ctgtttctcg cgtccgacga ggcgtcgttc
      241 atcaccggcg tggtcctgcc ggtcgacggt ggttacctgg cgcaataacc ggcatcctgg
      301 cctggattga tgcatcgggt tgccgcgctg gaacgtacgc cattgcggga agaatctccg
      361 agtcgacgac tggcccgccg tttgccgaag ggcgagggat tgacggctat acttgccggt
      421 cgggttcgcc gatgctggcg tctacgctcc cgtccgttgt catcgccctg gtgatgagca
      481 gcatctgccc ggctgacgtg cgaaccgggt gatcactgga tccgatgtcc gaaacagcac
      541 agtcgatcgc gtcccaagat acagtgacac tgcccggcgc attgccgggg gttacgcctg
      601 gtgacgcccg cgccagagtg cgcgaactgg tcgaccgcgc ctggttcccg gatacgctcg
      661 gcgtgatcgg ggtgctgctg gtgacggcgt tgctggcctg gcaccggctc tggttggaga
      721 acggcctcgc gtatctcgat gtcgctacct tctacatgcc ctggtacgcg cacctcggcg
      781 aagcggtccg tgcgctcgat atcccgggtt ggaacccgta cgtcttctcc gggaccccat
      841 tcgccggcga tccccaatcc ggctggtggt acttcccggc gatggcgatc ttcagcctct
      901 tcgagccggt ccgcgcctac cagatcttcc tgatctttca cctcgccttc gctggcgtga
      961 ccacctacat cctctgccgg atgtacgggc tgcaagtcat tccgagcctg gcggccggcg
     1021 ctgcgtacca atccggacca ttcgtctcgc atgtcagctg ctgcctgatc cacgttcagc
     1081 tcgcgacctg gattccgatg gccctgatcg gcgtcgagac gactgtgcgc tcgaaacaca
     1141 cgaaccggcg agcgattggc tggatcatca ccgcctttgc gatgagccag atgatcgccg
     1201 gctggccagg gcagggcatg tacaacggtt gcctgctgac gggcgcctac ctcgccttcc
     1261 ggatcctgct ctcgcgggag aacggcgtgc tgggctggcg ggaccggatc gtgcggctga
     1321 tcggcgacag cgctggggtg ctctcgttcg cgctgctctg ggccgctgcg ggcatcctcc
     1381 cgcggatcga catcgtcagt cgcaccaacg tcgcctgggg cgaatacgac ggcttcgaga
     1441 ccaacagcta cgcgtccggc tggcagatct acacggtgct cgaccgcctc ttctccgaca
     1501 acaacggcta tcgctcactg ctgttctacc tgggcgcgcc gatcatcatt ctgatgttca
     1561 tcggcatcgt gctggcctgg cagcgaccgt gggtgaagtt catgaccggg gtcacgatcc
     1621 tgacctcgat catgacgctt aagccgacct tcatccacga gatcgtcttc ctgctgcccc
     1681 gatacgaggc cctgcacaac catgtgccga gccggatcct cgctgtgcag tggatcggcc
     1741 cggtggttct cgcggcgact gcgctcgata cgctcatgcg ctcgccatcc cgggatcgga
     1801 tcctgcgcgc ggtcgggatc gccttcggga tctggaccgg cgtggccctg ctcctgcaag
     1861 tcggaaatcg gccggtgccc ggagcggtga tcgtggccgc ggtgatgacg gccggcgtcg
     1921 tctgcctctt cgcgatcatc accacgccgc caagcgatcg ctacagccgc ttcgtgccac
     1981 gcgcgcaggc tggcctcgcg atcctgctga tgctgctcgt gattgccgat ccggccgggc
     2041 gacggctcgt ctcgacgctc gagaccgggc agacgatgtc cgaggtgatc cgggttccga
     2101 ccggcccgat cagccggcaa gcggtgccgg acaacgatgc ctcgaccgac ccgggcggcg
     2161 cgggtgagtt cctgcaggcg aagcgggacg agggtgagta tttccgctac ttcggctacg
     2221 acaacgtcct gcagcagggt ggcgaaggat acccgtcgac gtatcgcgag aactttggga
     2281 atccgactgc aatctcgatt ctggtcaatg cccgggcgat gcgcctgcac ctgaacgatg
     2341 tccagggtta caacccggtt caactcaaga actatgtcac gtatctgaac gagctgaacg
     2401 gggccgtcca gaactaccac gacgcgcaga tcttgccggg cggtctgaac tcgccgctac
     2461 tgaacatcct gaatgcgcgg tacatcgtca ttccaaatga ggcgattggc cagcgcccgc
     2521 gtgccgatat catgctgctg ctagcctcgt atccggaggt tttccgcagc gacaccgtgc
     2581 gaatacttga gaacccgcat gcaatgccgc gggtctggat cgcccacaat gtcgtgcagg
     2641 ctcagccgga gctggccgcg gccatgatcg aagcgcccgg cttcgatccg gccgagaccg
     2701 ttatcctgag tcctgtcgcg ggcccggtcg cggcgacgca gccggcgaat cccaaggcag
     2761 aatcggttca gatcgtcacg tacgagccgg atcgggtcgt cctgaaggtc catgccgaat
     2821 ctgacggtgt ccttgtcctg agcgagacgt acgaggaggg ttggcatgcg cacgtcgatg
     2881 gcgacaaggc gccggtcgac gaggcctacg gcgtggtccg cgctgtgccg gtcacggctg
     2941 gcgatcacac ggtcgtcctg acctacgatc cctggtcgct ccggtatggg ttttatctct
     3001 cgttgcttgg cgccgcgctc tcgctggcgg tgatcggcgc atatgctgtg cgccggatgc
     3061 gcggtggcgc gttggaggtt gatggttgat gagtgacgag caggggcctg tgcgcccgcg
     3121 agcgaaccgg acgccccggc gccgggaacc gcgaaggctg gtcgccagcc cgcatcgttc
     3181 cggccgtacg ctcgcgatcc tggccgcact cgtgatcgcc tcgatcttca tcagttacga
     3241 cgttgccggg attgccgatc tgcaaaagga tccggtgacc ggcgtcgatt acgtgccggt
     3301 ggtcaactac gtgcaggcgc ggcgccagcc cggcgagaag gtgctggtcg cgctgccgcc
     3361 accggcctac ctggccttca aatcgaccga cgacctgatc ttcctctcca gcccgatctc
     3421 ccggaagcgg gcgcagcgtt atacgcgcct gaccgaggac ggcggctatg tcgagtactg
     3481 gaccggcgtg gactccgtcg tcgatacggc ctcgctttgc cagactcttt acaacgagcc
     3541 ggggctttgg ctgatcgtcg atgaggcccg gctcaacgcc gactgggcgt tccagggttc
     3601 gatggcgacc gtgatccgcg gtctgaccta tgtccggtac gaggcagagg gcggtgcgat
     3661 ggtgcggcgg ctggcgcccc tcccatcccg ggatccagtc gccgagcagc tctgcgccga
     3721 ggcccagacc ggcaaggtca ccgactctga cgagatcgtg gacttctcac aacccgagcc
     3781 aactccgact cccgaaccgt agccccggcc ccccgtggcc ggctgggtcg acggtacaca
     3841 gcgttgatag agcagacctg cttcctcaac cgactcgtcc gccaccgaag cggcccagag
     3901 gcggtccgcg ctacgtcgta tggcgcacgg tcaggggcgc ggggggatct ggatcttgcg
     3961 cagcgacggg ttgatgaagg tcatctcgcc attgcccagg ttgcggagcg tgattcgcac
     4021 ctgatccgtg tcggccgggc agaagatcgc cgattggtga tgcatcacgt cgttgacccg
     4081 ctcgcccgat gtccccgcgg tcagctcgct gagcacgccg ccggcctgcg tctggcaggt
     4141 cagcgtgacc aatgcccgcc cgccggtcag ccgggtctgg acgtcgacat cgaacgtgta
     4201 gagcgccgcg ccgccggttt tgatctgcgt cacgaccgca gtgccggtgc gggactggtt
     4261 cgtgatatgc acagctgtcc catcctccgt cggctcgacc ggtgataggc cggttggaat
     4321 gccccaggcg gtctcgttgc tgaacggctg catgccgggg acgacatcgc cgaggttcgc
     4381 gccgggccgg gcatagagat cgaggaagac gaggccgcgg ggcgcatcgt agaccttgtc
     4441 gatgacgcgc acgtagcccg cgttctcaat cccggcacgg acgaactcct cgccgcgtcg
     4501 ctggtaatag aggtaccaga tcacatcgtt gtcgggcgtc tgcggcagga gttcgtcgag
     4561 cttgccggcg tcgacgtccg cgatcgtgat ccccttctgg gggatcgtgt cgttcgaaag
     4621 cgtcttctcg agctcgccgt cacggaccgt gatcagccgc atcgtgtcga gcaggcccgg
     4681 ttcgtaggcc tcaaccagcg tatcggctac cgctccgtac gagaaggtca ccaccgggta
     4741 gttgagcgta ctgaccgtgg ccaggtcggc tgaggcatcg ttccagcgct g
//
LOCUS       DATAOZ010000477         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8611869, whole genome shotgun sequence.
ACCESSION   DATAOZ010000477 DATAOZ010000000
VERSION     DATAOZ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8611869"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..995)
                     /locus_tag="VHR64_12230"
     CDS             complement(165..995)
                     /locus_tag="VHR64_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX3303643.1"
                     /translation="MTSTTAAGPGNNGQSMVQIAGVHKFFGDLHVLRGVDLEIAPGEV
                     VCVIGPSGSGKTTLLRCINFLETYQQGRIYVDGQLVGYRDVRGHLKPAKERDVARVRA
                     ETAMVFQSFNLFPHMTALKNVTFGPMKVLHEPKADVERRGRELLERVGLADTADAYPA
                     QLSGGQQQRVAIARAVAMNPKVMLFDEVTSALDPELVGEVLAAMKQLAETHGVTMIVV
                     THEMAFARDVADRVVFMDQGVVVEQGPPAEVFTNPKSDRLRAFLARFSGSHATIPVAT
                     "
     gene            complement(1009..1686)
                     /locus_tag="VHR64_12235"
     CDS             complement(1009..1686)
                     /locus_tag="VHR64_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3303644.1"
                     /translation="MQVVRDNADLIWIGLKLTLELSIVIIALSTLIGAFVGIGLVYGN
                     WLVRSVLRLYVDIIRGIPLLVLIFLIFYGLPALHISILGHTINTNIGRFETATLAFTL
                     FAAAHVGEIVRGALAAVPAGQTDAAKAIGLTFWPRLRHVLLPQSIPVILPPWTNTAAE
                     LVKGTSLVTLVSMSDLMFATRKIAERTGEVVPLYAAAAVIYFVICFSISRAGVWVSGR
                     YRYGVAR"
     gene            complement(1690..2385)
                     /locus_tag="VHR64_12240"
     CDS             complement(1690..2385)
                     /locus_tag="VHR64_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX3303645.1"
                     /translation="MYDLRWEYITNNFGLFTDGLLLGLKMAVIALAVGSVIGLVCAFV
                     RSSGNRWASVPVTIYVELIRNVPLLLIVFLLYFGLPQAFPRQSAAHEWTLRLLPDAER
                     TFTVALAIYAGAYLTEIFTAGILSVGRRYLDAGRSLGLSRLALARYVTTPIMLRAVLP
                     SLSNTFISLFKDTSIAVAIATPELTMAARKISTNYFRVIEAWTTAGALYLVTSYAIAI
                     SLRMVERRIKWSV"
     gene            complement(2460..3380)
                     /locus_tag="VHR64_12245"
     CDS             complement(2460..3380)
                     /locus_tag="VHR64_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX3303646.1"
                     /translation="MEERFRAMASRRGLITRGAALTGAAAVTMGVTPHVARAIEDPSQ
                     SKLKQILDAGKIRVGTGSTNPPWHFEDENGKLVGFDIEMAKIVAGGLFGLTQEQVLGD
                     DEVRKHIEFVVHEADARIPDLVADKVDVNFQFMTVTAGRALQVEFTIPYYREGVGLLF
                     LKDSPNNTRADLEGKGANISILQNVYSEEIVHRGVSDANVQQFDSVAAAIEALDSGRV
                     DASASDLSTLAWLAKNNPDKYKVDTNGWDPNSYAASIKPGDQIFLNFLNTAIQESMVG
                     LDFPLYKQAFSTYFGVELDPAPTGIPVEWR"
BASE COUNT          693 a         1074 c         1220 g          666 t
ORIGIN      
        1 gcggtacggt agtcctcgct cgggaacacg tgcgccaacc tcaagtcgta tacgagtgcg
       61 gcggtcacct cgatgctggg aatggcccgg gtgaagggga ttccgccgca atgagtccag
      121 gagtcgagca accggaaggg atttaccgtc tgccgccgtg gctgtcaggt agcgacgggt
      181 atcgtggcgt ggctgcccga gaagcgggcg aggaaggcgc ggaggcgatc gctcttcggg
      241 ttcgtgaaca cctctgcggg cggtccttgc tcgaccacta cgccctggtc catgaagacg
      301 acccgatccg ccacgtcgcg ggcgaaggcc atctcgtgcg tcacgacgat catcgtcacg
      361 ccatgggtct cagcgagttg tttcatcgcg gccagtacct cgccgaccag ctcgggatcg
      421 agggcggagg tgacctcgtc gaagagcatc accttcggat tcatggccac cgcgcgggcg
      481 attgcgacgc gctgctgctg gccacccgag agctgggctg ggtaggcgtc ggccgtgtca
      541 gcgaggccga cgcgttcgag cagctcgcgt cctcggcgct caacgtccgc cttcggctcg
      601 tggaggacct tcattgggcc gaacgtgacg ttcttgagtg cggtcatgtg agggaagagg
      661 ttgaagctct ggaaaaccat tgcggtctcg gcgcggacgc gggcgacatc gcgctccttc
      721 gccggcttga gatggccacg gacatcgcgg tacccgacga gctgcccatc gacgtagatg
      781 cggccttgct gataggtctc gaggaagttg atgcaacgta gcagagtcgt cttgccggac
      841 ccgctcggcc cgatgacgca gacgacctcg ccgggcgcga tctcgagatc aacgccacga
      901 agcacgtgca ggtcgccaaa gaacttgtgc acgccggcga tctgcaccat cgactggcca
      961 ttgttgcccg gtcctgcggc cgtcgttgaa gtcatgaagt tctcccgatc accgcgctac
     1021 gccgtagcgg tatcgcccgc tgacccagac accggcgcgg gaaatcgaaa agcagatgac
     1081 gaaatagata accgccgccg ccgcatagag cggaactacc tcgccggtgc gctcggcgat
     1141 cttgcgagtc gcgaacatca ggtcgctcat gctgacgagc gtgaccagcg acgtgccctt
     1201 gaccagctcg gccgctgtgt tcgtccaggg cggcagaatc accgggatcg actggggtag
     1261 gaggacgtgg cggaggcggg gccaaaaggt gaggccaatt gccttggccg catcggtctg
     1321 gccggctggg accgccgcca gtgcgccgcg cacaatctcg ccgacgtggg ccgccgcgaa
     1381 tagtgtgaat gctaacgtcg ccgtctcgaa gcggccgata ttggtattga tcgtgtggcc
     1441 aaggatcgag atgtggagcg caggcagtcc gtagaagatg aggaagatca gcaccagtag
     1501 cgggatgccg cggatgatat cgacgtagag tcgcagtacc gaccgcacga gccagttacc
     1561 gtagaccagc ccgatgccga cgaatgcgcc gatgagcgta ctgagcgcga tgatcacgat
     1621 tgataactcg agcgtgagct tgagaccgat ccagatcaga tccgcattgt cgcgaacgac
     1681 ttgcatagcc tagacgctcc acttgatgcg ccgctcgacc atgcggaggc tgatcgctat
     1741 ggcatatgag gtgaccaggt agagcgcacc ggcggtagtc caggcctcga tcacgcggaa
     1801 gtagttggtg ctgatcttgc gggcggccat ggtcaattcg ggcgtcgcga ttgcgacagc
     1861 gatcgacgtg tccttgaaga gcgagatgaa tgtattgctc aagctcggca gtaccgcgcg
     1921 cagcatgatc ggcgtggtga cgtaacgagc cagggcaagc cggctcagcc cgagcgatcg
     1981 accggcatca aggtagcggc gaccgaccga aaggatgccg gcggtaaaga tctctgtcag
     2041 gtaggccccg gcgtagatcg cgagcgcgac agtaaaggtg cgctctgcat caggtaggag
     2101 gcgaagggtc cactcgtggg cggcgctctg ccgcgggaat gcttgcggca gcccgaaata
     2161 gagcaggaag acgatcagga gcagcgggac gtttcggatc aactcgacat agatcgtgac
     2221 cggcacgctt gcccagcgat tgcccgagga gcggacaaag gcgcaaacaa ggccgatcac
     2281 tgagccgacg gcaagggcga taaccgccat cttgagcccc agcaggagcc cgtcggtaaa
     2341 caggccgaag ttgttggtga tgtattccca gcgtaggtcg tacacgaacg atccggttca
     2401 aacaggccgt cgccccgggt gtgtcaacca cccgggacga cattgccgtc tagctctcgt
     2461 taccgccact cgaccgggat cccagtcggt gccgggtcga gttcaactcc gaagtaggtg
     2521 ctgaacgcct gcttgtagag cgggaaatcg agtccgacca ttgattcctg aatcgcggta
     2581 ttcaggaaat tcaggaagat ctggtcgccc ggctttatag aggcggcgta ggagttcggg
     2641 tcccagccgt tcgtgtcgac cttgtacttg tcggggttgt tcttggcgag ccaggccagt
     2701 gtcgagagat cggaggcgga tgcgtcgacg cgaccggagt cgagcgcctc aatcgcggcc
     2761 gcgactgagt cgaactgctg gacattggcg tctgagactc cgcggtggac gatctcctcg
     2821 gagtagacgt tctgcaggat cgagatgttg gctcctttgc cttcgaggtc ggcccgcgtg
     2881 ttgttgggcg agtccttgag gaagaggaga ccgacgccct cacggtagta gggaatcgtg
     2941 aactcgacct ggagcgcgcg gccggcggtc acggtcatga actggaagtt gacatcgacc
     3001 ttgtcggcta ccaggtccgg gatacgggca tccgcttcat ggacgacgaa ctcgatgtgt
     3061 ttgcggacct cgtcgtcacc cagtacctgc tcctgagtga gaccgaagag gccgccggcg
     3121 acgatcttcg ccatctcgat gtcaaagccg acgagcttgc cgttctcgtc ctcgaagtgc
     3181 cagggcggat tggtactgcc ggtgccgacg cgaatcttgc cggcatcaag gatttgcttg
     3241 agcttgctct ggctcgggtc ttcgattgcc cgcgccacgt gcggggtgac gcccatcgtg
     3301 acggctgccg caccggtcag cgccgcgccg cgagtgatca gcccgcgtcg actggccata
     3361 gcacgaaagc gctcttccat cgcgaccttc tccttccctg tgggccggcg gagcgaacgc
     3421 cgcggcgtcg ccaccggctc ctttgactcg atcggcggac cgcgtggtcc gttggctgag
     3481 acgcccggat gctatggcct gctcgcgaag ctgtcaacag gggtgttcgt taccaattgc
     3541 gcggtgcaat cgacccgcgt gagacggctg ccaatgacac gccggtcatg ccatgaggtt
     3601 gcggcgcgtt ggcgcgcgac atcctcattt gctgcacatg ctcggcgcaa ggt
//
LOCUS       DATAOZ010000478         4898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7562258, whole genome shotgun sequence.
ACCESSION   DATAOZ010000478 DATAOZ010000000
VERSION     DATAOZ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7562258"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VHR64_12250"
     CDS             complement(<1..314)
                     /locus_tag="VHR64_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303647.1"
                     /translation="MLTYNFAMENQPLGDQIEISWAACPRWATFESADLVDAVEQLGG
                     RWAILLVDVQGSGRGGRRLASILAREARELLAAGISAETAVRAVHEHLHELRQGKVGA
                     SI"
     gene            862..2220
                     /locus_tag="VHR64_12255"
     CDS             862..2220
                     /locus_tag="VHR64_12255"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX3303648.1"
                     /translation="MHVGSAQSNVISLQASAESTGSGEIAERWYGPALALVTVALILM
                     SVVRGYFLAPDAHLALSLIVAVLLIVGGELFIISLPFSWGTAQVSVGAICGLGVAIWL
                     GPAYAPAMILIVALLSSYRQWKHFGLALENAASAAVAAAAAGMVWWALIPDHANPLTS
                     IRTTVVFLLVAVLHSVVNFGTVVVGTALARREPVLKLIYTMSGGKNFIFTIPVLAAFI
                     PIIGSQSPIALLFFAIPLVSSHLALRALWRLETDTQTTLAALTDILELRDPYTAFHSE
                     RVSIYAVAIAQHVPELTAKELATLERAARIHDIGKAAVRDAVLLKNGPLTDEERRSIE
                     SHSSIGADLIARMHAYRDCVELIRHHHERWDGGGYPSRLIGDRIPLGARIMAVADSLD
                     AMTTDRPYRKALSFESAVAEIRRNSGTQFDARIVDAFFAAINDPVFERLGEDSHSPLA
                     AD"
     gene            2259..2999
                     /locus_tag="VHR64_12260"
     CDS             2259..2999
                     /locus_tag="VHR64_12260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3303649.1"
                     /translation="MGSAITFDFHNTLIRCDRWFDLEVAGLPGEVADALASLGGGLPL
                     QQRQQLTDTYGALRAEIIAHGNELDATDGVLETFARLGYVVRRDVVRPIVDSLFLPLV
                     AESEVIEGVRETLAHLAELGFAIGVVSSAVHHDFLEWTLEHHDLRSYLADVTTSASSG
                     YYKSRPEIFRAACERLGATPERTVHVGDSFRFDHLGGSAAGLRTVWLNQSGIERPSGE
                     PSPDLELASLVGAGPLIEQILMATVDAR"
     gene            2989..3732
                     /gene="trmD"
                     /locus_tag="VHR64_12265"
     CDS             2989..3732
                     /gene="trmD"
                     /locus_tag="VHR64_12265"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX3303650.1"
                     /translation="MRVDVLTLFPDLVDAPLSASIVGRARQSGVLDLNIHDIRTWTSD
                     VHRTADDTPYGGGPGMVMKAEPIVSGAEWIAETHGTPDRIYILSASGQLLTHDLAVDI
                     AEQGHVLLICGHYEGIDDRVRLLLSAEELSIGDFVLTGGELAAAVVVDCVTRLIPGVI
                     KAESVAEESHAAGLLEYPHYTRPATFRGLAVPDILLSGNHAEIDRWRYRQALARTAEN
                     RPDLVSKLSEADRQALVRLGVAVRTSEQE"
     gene            3893..>4898
                     /locus_tag="VHR64_12270"
     CDS             3893..>4898
                     /locus_tag="VHR64_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3303651.1"
                     /translation="MATTGLLERAAAPEAATVAREPQSVEQTGLDLSSITDLVLKFIY
                     FNNQITAQSISDDVCLPFYNIVDRALMVLKKEELVEVAGSNGFGELAYQYSITPKGVV
                     RVHQILERTTYVGPAPVTVDHYRRVIMAQAISTVRVGPNEVREALSDLVLEDHVMDAM
                     GQAVNTGRSLFLFGPPGNGKTMLAEHIVPLLGGEVLIPHAFTVDGQIIKVLDLHNHVP
                     VAVNGRPDFDRRFVLCKRPAIITGGELMLEGLDLVYDKFARIYEAPLQLKSNGGMLLI
                     DDFGRQQVSPRQLLNRWIVPLEKRVDYLTLHTGKKIEIPFDQLIVFSTNLQPKDLVDE
                     A"
BASE COUNT          889 a         1535 c         1485 g          989 t
ORIGIN      
        1 tggatggacg cgcccacctt tccctgccgg agctcatgga gatgttcgtg cacggcccga
       61 accgcagtct cggcagagat cccagcagcc aacaactcgc gcgcctcgcg ggcgaggatg
      121 ctagccaagc gacgaccacc acggccggat ccctgcacat ccacaagcag gattgcccac
      181 cggccgccga gctgctcgac ggcatcgacg aggtcggcgc tctcgaacgt ggcccagcgt
      241 gggcaggccg cccagctgat ttcgatctga tcgccgaggg gctgattctc catggcgaaa
      301 ttgtaggtga gcaatgccag tcccggtccg ggtagccgac tgggctagga atatggtcat
      361 ggggtcctgt tgacatgacc ccccaaaggt cctatattgg ctgtacagac gtcacagagg
      421 gcggaggcga aagtttccgg tggcggatga cggggagggg cctcctcgga tgggaatggt
      481 gccgaagtac tcggctcgga aggtgcacag tagacatggt tcgctcgaat cactggggcc
      541 gcggcctgcc gtaacccgac tcggactctg tacctggcga gctcacgatt gccgcacagc
      601 gtccgaagtc gatcctcgcc agctctgccg ccggcgagta ttgcctgaat tgggacagag
      661 ggggcccggc cgcacaggtc gggccccgtc gcatgtccaa atggctcgcc gacaggtgcc
      721 gtccggacta gtacctcggt agggaatcgc aaaaacgtgc cccaaatcgc gcgcgagcac
      781 aatatcactg cccagatttg gtccatatac tgccagaggg tagggaagct accgtcgcac
      841 accagaatct ctggctccag gatgcacgtc ggatcagccc aatcgaatgt catctcgctc
      901 caggcgagcg ctgagtcaac cggttctggc gagattgccg agcgctggta cggtcccgct
      961 ttggccttgg tgacggtcgc tttgatcctg atgtccgtcg tgcggggcta cttcctggcg
     1021 cctgatgcgc atctcgcact ctcactcatc gtggccgtgc tgctgatcgt cggtggagag
     1081 ctgttcatta tctcgctccc attcagttgg ggcacggcgc aggtttcggt cggggcaatt
     1141 tgcggattgg gcgtggccat ttggctcggt cccgcgtatg cacccgcgat gatcctgatc
     1201 gttgcgttgc tgtcctcata tcggcaatgg aagcacttcg gactagccct ggaaaatgcg
     1261 gcttcggcag cagtcgccgc ggctgcggcc ggcatggtct ggtgggcgct gatacccgac
     1321 cacgcgaacc cgctgacctc gatcagaacc accgtcgtct tcctcctggt ggctgtgctg
     1381 cactcggtcg tgaactttgg cacggtggtc gtgggcacgg ccctggcccg gcgcgagccc
     1441 gtcctcaagt tgatctacac gatgtccggc ggaaagaact tcattttcac gattccggtc
     1501 ctagccgcgt tcatcccgat tatcgggagc cagagcccga tcgcactcct cttctttgcg
     1561 atcccgctgg tcagctcgca ccttgccttg cgtgcgctct ggcgtctcga gaccgatacc
     1621 cagaccacat tggcggcgtt gaccgacatt ctggagctca gggacccgta cacggccttt
     1681 cattcagagc gagtatcgat ttacgccgtg gcgatcgcgc agcacgtccc cgagttgacc
     1741 gccaaggaat tggccaccct cgagcgcgcc gcccgcattc acgatatcgg caaggctgcg
     1801 gttcgtgacg ccgtgctgct caagaacggg cctctaaccg acgaggagcg ccggagtatc
     1861 gaatcgcact cgtccatcgg cgccgacctg attgcccgga tgcatgccta ccgggattgt
     1921 gttgaactta ttcggcacca ccacgagcga tgggatggcg gtgggtatcc atcgcgcctg
     1981 atcggtgatc gcattcccct tggtgcgcgg atcatggccg tcgccgactc actcgatgcc
     2041 atgacgaccg atcgccccta tcgcaaggcc ctctcgtttg aatcagcggt cgcggagatc
     2101 cggcggaaca gcggaaccca attcgatgcc cggatcgtcg atgccttctt tgcggcaatc
     2161 aatgatccgg ttttcgagcg attgggagag gattctcact ccccattggc ggcagactga
     2221 tcgctcccgt acccttcacg agacagggaa ggggctggat gggatccgca atcacgttcg
     2281 attttcacaa cacgcttatc cgctgcgatc gctggttcga cctggaagtt gccggcctgc
     2341 ccggcgaggt cgccgatgcg ctcgcctcgc tcggcggtgg tctgccactg caacagcgtc
     2401 agcaattgac cgacacgtat ggagcgttgc gggcagagat catcgctcat ggaaatgagc
     2461 tggacgcaac ggacggcgtg ctggagacgt ttgcgcgatt gggatatgtc gttcgccgcg
     2521 acgtcgttcg ccccatcgtg gacagcttgt tccttccgct cgtcgccgaa tcagaagtca
     2581 tcgaaggtgt tcgcgaaacc ctcgcgcatc ttgcggagct cggctttgct attggggttg
     2641 tttcgagcgc ggtgcaccac gactttctcg aatggaccct cgagcatcat gacttgcgct
     2701 cgtacctcgc cgatgtgacg acctcggcaa gttccggtta ctacaagtcg cgaccggaga
     2761 tcttccgcgc cgcatgcgag cgcctcggag ccacgccgga gcgcacggtg catgtcggtg
     2821 actcgttccg ctttgatcac ctcggtggct cagcggccgg ccttcgaacc gtttggctga
     2881 atcagtccgg gatcgagcga ccgtccggag agccatcgcc cgacctggag cttgcctcgc
     2941 ttgtcggcgc cggtccgttg atcgagcaga tcctcatggc cacggtcgat gcgcgttgat
     3001 gtcctgaccc tgttcccgga tctggtcgac gccccactct cggccagcat cgtcggacgt
     3061 gcccgccaga gcggcgttct ggacctgaac atccacgaca tccggacttg gaccagcgac
     3121 gtgcaccgca cggccgacga cacgccgtat ggcggcggtc cgggtatggt catgaaggcg
     3181 gaaccgatcg tctccggagc ggagtggatc gccgagaccc atggcacgcc agatcgcatc
     3241 tatatcctct ctgcatcagg acagctgctg acgcacgacc tcgcggtcga tattgccgag
     3301 caaggccacg tgctgctcat ctgcggccac tacgaaggga ttgacgatcg cgttcgcctc
     3361 ctgctttcgg ccgaagagct gtcgatcggc gactttgtgc tgactggcgg cgagctggcg
     3421 gcggcggtcg tcgtcgactg cgtcacgcgt ctgattccag gggttatcaa ggctgagtcg
     3481 gtcgccgagg agtcacacgc agcaggactc cttgagtacc cccattacac ccgtccggcg
     3541 accttccggg gcctcgccgt ccccgacatc ttgctcagcg ggaaccacgc cgagatcgac
     3601 cggtggcgct atcggcaggc gcttgcacgt accgcagaga atcgccctga tcttgtgtcg
     3661 aagctctccg aggcggatcg gcaagctttg gtcaggttgg gggtggctgt ccgtacttcc
     3721 gaacaggagt agcatccggt gtcgggactt tggcgctatg tgaatcgggc tatccccaga
     3781 gtagcctgat cgtaagaaga cgatatcgag cgcgggcaat gcgtcccaca tcctgacgct
     3841 caccgaattc gaacttgttc agtacgcatt tggtgaggag acctcatccc acatggccac
     3901 caccggactc ctggaacgag cggctgcacc cgaggccgca actgtcgcac gcgagccgca
     3961 gtccgttgag cagactggcc tcgatttgtc gagcattact gatctggtgc tcaagttcat
     4021 ctacttcaac aaccagatca ctgctcagag catttcagac gacgtctgcc ttcccttcta
     4081 caacatcgtc gaccgagcgt tgatggtgct caagaaagaa gagttggtcg aggttgccgg
     4141 ctcgaacggg ttcggtgagc ttgcctatca gtattcgatc acgccgaaag gcgttgttcg
     4201 ggtccatcag atcctggagc gcacgaccta cgtcggaccg gccccggtca cggtagatca
     4261 ctaccgcagg gtgatcatgg cccaggcgat ctcgacggtt cgagttgggc caaacgaagt
     4321 ccgtgaggcg ctctccgatc tggtcctcga ggaccacgtc atggatgcga tgggccaggc
     4381 agtcaacacc ggccgctcac tcttcctgtt cggacctcca ggcaatggca agacgatgct
     4441 ggccgagcat atcgttccgc ttctcggtgg cgaagtgcta atcccgcacg ccttcacggt
     4501 tgacgggcaa atcatcaagg tcctggacct gcacaaccac gtgccggttg cagtcaacgg
     4561 gcgaccggac ttcgaccgcc ggtttgtgct ctgcaagcga ccggcgatca ttaccggcgg
     4621 cgagctcatg ctcgagggac tggacctggt ctacgacaag ttcgcgcgca tctacgaggc
     4681 gccgctccag ctcaagtcca acggcggcat gctgctgatc gacgacttcg gccgccagca
     4741 ggtttcgcct cgtcagctgc tcaaccgctg gatcgtgccg ctggaaaagc gcgtcgacta
     4801 cctgacgctt cataccggca agaagatcga aattccgttc gaccaactga tcgtcttctc
     4861 gaccaacctg cagccaaaag acctggtcga cgaggcgt
//
LOCUS       DATAOZ010000479         6913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6243225, whole genome shotgun sequence.
ACCESSION   DATAOZ010000479 DATAOZ010000000
VERSION     DATAOZ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6243225"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VHR64_12275"
     CDS             complement(<1..920)
                     /locus_tag="VHR64_12275"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX3303652.1"
                     /translation="MSSDQITVVIGSPLEAEHVARISAVAPDRVEVVYEPELLPPARY
                     IADHDGPPEWARSDADQTRWMEILASADVLFCLPREAQSDLLAICPKLKWLQGTSAGM
                     GQPAQRLGLIDSDVIVTTASGVHAGALSEFVFAALLSRTRMLGQLQEWQRAHHWQRFT
                     GGELAGKQMTIVGPGRIGRQIARVAHAFDMRVTAVGRTGGSERAEALGVDHYLPIGRL
                     HEALSTADVVVIVTPHTGGTERLIGAAEFEAMKPSVWFINIGRGAVVDEQEMIARIRD
                     GRIGFAALDVFQQEPLPPNSQLWDLPNTLIS"
     gene            1014..2111
                     /locus_tag="VHR64_12280"
     CDS             1014..2111
                     /locus_tag="VHR64_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX3303653.1"
                     /translation="MTATSVYQDRIARAQAAMRAAGVDLLCIGPSADLFYLTGINAHL
                     SERLNLLLVPANGQPKFVVPRLEAPNVADKTDLVEIKPWEETESPSDLVASLAGDAKS
                     VGVGDQLHAIFLLRLQEAIPGAKWKQGGPILRDLRMRKDDAEIAAMREVALRTDQAWA
                     AFLEGGPIEGLTEQQAMDRLNQLMTERGVTPMFGICASGPNSASPHYNTGDRVIKRGD
                     AVVFDWGGELNGYLSDMTRTIVIGEPSDEYRKVYEIVLRANRAAFDAVRPGVPCEDID
                     RAARDVITDAGYGEYFIHRVGHGLGLDVHEEPYMVSGNTLPLAPGMTFSDEPGIYMAG
                     KFGIRIEDTVVCTEDGAEFVNAAPRDLTVMD"
     gene            complement(2112..2606)
                     /locus_tag="VHR64_12285"
     CDS             complement(2112..2606)
                     /locus_tag="VHR64_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX3303654.1"
                     /translation="MDHVGEDTLAQLPPDLMTSDDSDLPADIDVPFTPAAQRVIERSV
                     ELADMVGGDAIVTEHLLLAITSTPECAAAQLIAEHDSSSESVLSALQSMLGDTSRLAS
                     ADQPQPRLERVIIRAKREAFRHRHPEVSTLHLLMALIRERAGAGYQKLAGPNRQAARA
                     GESD"
     gene            complement(2690..3871)
                     /locus_tag="VHR64_12290"
     CDS             complement(2690..3871)
                     /locus_tag="VHR64_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX3303655.1"
                     /translation="MAELTEAEALRRSLVAGKHAQAKELLRDLGIDCWLTFQREGSDV
                     MLPYVLGMNELVSQSALMIFADGPTVAIVMDYDATTVAGVFDEVIAYQSTWRDPLLTV
                     LQERNPARIALNYDMLDDGIDGLTYGQYLLLTEALKPIGFADRLESASPVTSMLRQIK
                     TPDEIERMRRACEITVHLFGDVTSILKPGLTEWDIYEYLHEQMKTYNVTASWDPAYCP
                     GVMSSKRASGHTPPTQIKLEPGDGLRVDLGVISEGYASDLMRTWYIRKPGEKSAPPEF
                     LRAFDTVRDAIQLAADTIRPGLSGIEVDTVARSFVEKAGYEFFHATGHQVGTRAHDGG
                     LLLGPDNARYGERSRGLIQAGMTFTLEPVFGPIGIEENIVVTPEGCTYLVPPQRELWL
                     I"
     gene            complement(3884..4606)
                     /locus_tag="VHR64_12295"
     CDS             complement(3884..4606)
                     /locus_tag="VHR64_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX3303656.1"
                     /translation="MDGQLLKKAIVEFIGTFSLLFMGIGAIVMTGGENLVAIAFAHGL
                     AIGLLIMAAGHISGGFFNPAVTIGMLVTRRISGYNAGVYIVAQLLGAVAGTLFILAAF
                     PESMRDAVNLGVPAVGTGYTTGNALIAEIVTTFFLIFVIFGVAVDQRSAKAVGGLAIG
                     LTITMDIFATGAISGAAMNPARWFGPAIVGGHWDDFWIWIIGPIVGAVVAALIFNDVL
                     LAGEGLEDERPSHLRNRRRVSR"
     gene            complement(4720..5271)
                     /locus_tag="VHR64_12300"
     CDS             complement(4720..5271)
                     /locus_tag="VHR64_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303657.1"
                     /translation="MAGCWCIALLSACASGNANSANEAATREAGRATNVVANIEATQV
                     MREFFDQHTSPTPYPTILPSLANLRLTTGLREEDAPTNTVYNYTRGSGPLYADAQVAN
                     VFPGQRVIAVWSTNGTVLQTTEINVENPHELIWVALRWDIPPDAPSGQYVVSIQVGGP
                     GTNDEGTPADVTTEIGTLVFRVN"
     gene            complement(5303..6379)
                     /locus_tag="VHR64_12305"
     CDS             complement(5303..6379)
                     /locus_tag="VHR64_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX3303658.1"
                     /translation="MAIEHDELDTFPGLAIDNDSVAAQTNGPLTITAAVEIFFGDLRL
                     APRSKKTYRNGIAKFVRHLADHEGIDPDVAPVTIISAEHVTSFASLIVPPDVRSPEEV
                     SQMRTAQNNLSAVRKFCSYLAAYDLHPSLSTDRLRVRIAAMMPRFTPPPPDVKLSDLE
                     RIVEFVRQPERDENEVKQLRAFKVRAMILMLFRTGVRVSELCAFRRRDIELADGQAHV
                     YRAKGGKSRTVHFDAETAQALQAYWNLRGDAIRGAGAAPAFSGRDKVGDAGNAISPRT
                     VEHIISQLCTTLGIESEITPHSFRHGLATEMVRRRVRESTVQTILGHASPQTTRIYVH
                     KSAIEVADEYQDAFGQYRPPSAAD"
     gene            complement(6483..>6913)
                     /locus_tag="VHR64_12310"
     CDS             complement(6483..>6913)
                     /locus_tag="VHR64_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3303659.1"
                     /translation="TLDTSRMYRTSLPQDPLHLIALNRDVFFGVRLRNLAKELGFDLV
                     LAANPDAVGRALHEHHQTAALVIIDMNVLGEGVDWETLGGILADYPAIPSLGFGSHTD
                     VETRRAAKQAGLTRIVANSEFHRDAAALIQRYARVAEDDE"
BASE COUNT         1235 a         2221 c         2244 g         1213 t
ORIGIN      
        1 gagatgaggg tgttcggcag gtcccagagc tgggagttcg gcggtagcgg ctcctgctgg
       61 aagacgtcga gcgccgcgaa cccgatccgt ccgtcgcgaa tccgggcgat catctcctgc
      121 tcatcaacga cggcaccacg cccgatgttg atgaaccaga cgctcggctt cattgcttcg
      181 aattcagccg cgccgatcaa gcgctcggtc ccgccggtgt gcggcgtgac gatcaccacc
      241 acgtcagccg tgctcagcgc ctcgtgcagg cgaccaatcg gcaggtagtg atcgacgcca
      301 agagcctcgg ctcgctccga accacctgtc cgcccgaccg ctgtcacacg catgtcgaag
      361 gcatgggcta cccgggcgat ctgccggcca atccggcccg gaccgacaat ggtcatctgc
      421 tttccggcca gctcgccgcc ggtgaaccgc tgccagtggt gcgcgcgctg ccattcctgg
      481 agttggccga gcatccgggt ccggctcagg agcgctgcga agacgaactc gctcagcgcg
      541 ccggcatgca ctccgctggc ggtggtcacg atcacgtcgg agtcgatcag gccaagccgc
      601 tgggccggct ggcccatccc ggccgacgtg ccctgcagcc acttcagctt cgggcagatc
      661 gcgagcaagt cggactgcgc ctcgcgcggc aggcaaaaga gaacatcggc cgaggcgagg
      721 atctccatcc accgcgtctg gtccgcatcg gagcgggccc attcaggcgg accgtcgtga
      781 tcggcgatgt agcgtgccgg cggcaagagt tccggctcgt agacgacctc gacccgatcc
      841 ggcgcgaccg cggaaatccg cgcgacgtgc tctgcctcga gcggcgaccc aatgacgact
      901 gtgatctgat ccgaactcac gcttccctcc gcatctgcag acctccagca ttctacgggt
      961 tggtggggcg atagaatccg caggtctgtt cgtatttcgc tcgggagagt ttcatgaccg
     1021 caaccagcgt ctatcaggat cggatcgcgc gcgcccaggc tgcgatgcgc gccgccgggg
     1081 tcgacctgct ctgcatcggt ccgtcggccg acctctttta cctgaccgga atcaacgccc
     1141 acctcagtga gcgactgaac ctgctgctcg tcccggccaa cggccagccg aagttcgtcg
     1201 tgccgcgcct ggaagcgccg aacgtggccg acaagactga cctggtcgag atcaagccgt
     1261 gggaggagac cgagtcgccg tccgatctgg tcgcatcgct tgccggcgat gcgaaatcag
     1321 tcggcgttgg cgatcagctc cacgcgatct tcctgcttcg tctgcaggag gcaatccctg
     1381 gcgcgaagtg gaaacagggc ggtccgattc tgcgcgatct ccgcatgcgc aaggacgacg
     1441 ccgagatcgc ggcgatgcgc gaggtcgcgc tccggaccga tcaggcgtgg gccgccttcc
     1501 tcgagggcgg accgatcgag gggctgaccg aacagcaggc gatggatcgg ctcaaccagc
     1561 tcatgaccga gcgcggcgtg acgccgatgt tcggcatctg cgcgagcggc ccaaacagtg
     1621 cctcgccgca ctacaacacc ggcgatcgcg tgatcaagcg cggcgatgcg gtcgtcttcg
     1681 actggggcgg cgagctgaac ggctacctct cggacatgac ccgtacgatt gtgatcggcg
     1741 agccgtcaga cgaatatcgc aaggtctacg agatcgtcct tcgggccaat cgcgcggcct
     1801 tcgacgcggt caggcccggc gtgccctgcg aggacatcga ccgtgccgcg cgggatgtca
     1861 tcaccgatgc cgggtatggc gagtacttca ttcatcgagt cggccacggt ctcgggctcg
     1921 acgtccacga ggagccgtac atggtcagcg gcaacacact gccgctcgcg ccgggcatga
     1981 ccttcagcga tgagccgggc atttacatgg ctggcaagtt cggaatccgg atcgaagaca
     2041 cggtcgtctg caccgaagat ggcgcggagt tcgtcaatgc cgccccgcgc gacctgaccg
     2101 tgatggatta gctaatcgga ttcgccggcg cgggctgcct gacggttcgg gccggcgagc
     2161 ttctggtacc cagcgccggc gcgctcccgg ataagggcca tcagcaaatg aagcgtgctg
     2221 acttcagggt ggcggtggcg gaatgcctcg cgcttggccc ggatgatcac gcgctcgagg
     2281 cgaggctgcg gctgatcggc acttgcaagt cggctcgtgt cgccgagcat cgattgcagg
     2341 gccgaaagga cgctttcgga ggacgaatca tgctcggcga tcaactgcgc cgccgcgcat
     2401 tccggggttg aagtgatcgc cagcagcaga tgctcggtca cgatcgcatc gccgccgacc
     2461 atatcagcca gctcaaccga gcgctcgatg acccgctggg cggccggggt gaacggcacg
     2521 tcgatgtccg cgggcagatc tgaatcgtcg ctcgtcatga ggtccggtgg tagctgggcc
     2581 agggtgtctt ccccaacatg atccataaga catccccctg ggcacttcgc tactggtggg
     2641 tcaatgttct ggagccattg tccgttattg aaaaaccgtt gtcgactccc tagatgagcc
     2701 acagttcgcg ctgcggcggt acaagatatg tgcacccctc gggcgtgacg acgatgttct
     2761 cctcgatccc gatcggtcca aagaccggct cgagggtaaa ggtcattccg gcctggatca
     2821 gtccgcgcga tcgctcgcca tagcgcgcat tgtccggccc gagcaagagc ccgccgtcat
     2881 gggcgcgggt cccgacctgg tgaccggtcg catggaagaa ctcatatccg gccttctcga
     2941 cgaacgagcg cgcgacagta tcaacctcga tcccggataa tccgggccgg atcgtgtccg
     3001 cggcaagctg gatggcgtcg cgcacggtgt caaatgcacg caggaactcc ggcggcgcgc
     3061 tcttttcgcc cggcttgcgg atgtaccagg tccgcatcag gtcggatgca tacccctcgc
     3121 tgatcacgcc gagatcgacc cgcaacccgt caccgggttc gagcttgatc tgggtcggcg
     3181 gcgtgtgccc tgaggcgcgc ttcgacgaca tcacgcccgg gcaataggct ggatcccagg
     3241 aggccgtcac gttgtacgtc ttcatctgct cgtgaaggta ctcgtagatg tcccactcgg
     3301 tcaggccagg tttgaggatg ctggtgacat cgccgaagag atgcaccgtg atctcgcaag
     3361 cccggcgcat ccgctcgatc tcgtcaggcg tcttgatctg ccggagcatg ctggtaacgg
     3421 gcgacgccga ctccagccgg tccgcgaagc cgatcggttt cagcgcctcc gtcagcaaca
     3481 ggtactggcc atacgtcagc ccatcaattc cgtcatcgag catgtcgtag ttgagcgcaa
     3541 tccgggccgg atttcgctcc tgcaggacgg tcaggagcgg atcgcgccag gtcgactggt
     3601 aggcgatgac ctcgtcgaaa acccccgcga cggtcgtcgc gtcgtaatcc ataacaatcg
     3661 cgacagtcgg tccgtccgca aagatcatca acgccgactg gctgaccagc tcattcatgc
     3721 cgagtacgta gggcagcatc acgtcgctgc cctcgcgctg aaaggtcagc cagcagtcga
     3781 tgccgaggtc gcgcagcagc tccttcgcct gggcatgctt accggcgacg agtgagcgac
     3841 ggagggcctc ggcttcggtc aattcagcca tgctgggcaa tccctatcga gacacacggc
     3901 ggcgattgcg gaggtgcgag gggcgctcgt cctccaatcc ctcgcctgcc aaaagcacgt
     3961 cgttgaagat cagggctgcc accaccgccc cgacaatcgg gccgataatc cagatccaga
     4021 aatcgtccca gtgaccaccg acaatcgccg ggccgaacca gcgcgccggg ttcatggccg
     4081 cgccgctgat cgcgccggtc gcgaagatat ccatcgtgat cgtcaacccg atcgcgaggc
     4141 caccgaccgc cttggcgctc cgctgatcga cggcgacgcc gaagatgacg aagatcagga
     4201 agaaggttgt gacgatctcg gcaatcagcg cattgccggt cgtgtagcca gttccgactg
     4261 ccggtacgcc gagattgaca gcatcgcgca tggactccgg gaaggccgcg aggatgaaga
     4321 gcgttccggc cacggcgccg agcaactggg cgacgatgta gacgcccgcg ttatagccac
     4381 tgatccgccg cgtgacgagc atcccgatcg tgaccgccgg gttgaagaag ccgccggaaa
     4441 tgtgccccgc tgccatgatc agcaggccga tcgccagacc gtgcgcaaat gcgatcgcga
     4501 ccaggttctc tccccccgtc atgacgatcg cgccgatgcc catgaagagc aacgagaacg
     4561 tgccaatgaa ctcgacgatg gccttcttga gcagttgccc gtccatccag accccttccc
     4621 ggcgcgacgc gttccgcccc cgctgcgaac tatctgatgc gcctgtcttc ccgcggggac
     4681 tctaccacgc gacggaagcg ggcgaaatcg acgccgggat cagttgacgc gaaagacgag
     4741 tgtgccgatc tcggtcgtga cgtcggccgg cgtgccttcg tcgttcgtgc ccggtccgcc
     4801 gacctggatc gagacgacgt actggcccga cggcgcgtcc ggcgggatat cccagcgcag
     4861 ggcgacccag atcagctcgt gggggttctc gacgttgatt tcggtcgtct gcagcaccgt
     4921 cccattcgtg ctccagacgg cgatcacccg ctgacccgga aagacgttgg ctacctgggc
     4981 gtcggcatag agcgggccgg agccgcgcgt gtagttgtag accgtgttgg tcggggcgtc
     5041 ttcctcgcgc aggccagttg tgaggcgcag attagcgagg gacgggagga tggtcggata
     5101 gggcgtcggc gaggtgtgct ggtcgaagaa ttcgcgcatg acctgcgtcg cttcgatgtt
     5161 ggcgacgacg ttcgtggccc tccccgcttc ccgggtcgct gcctcattgg cgctgttggc
     5221 attacccgat gcgcaggccg aaaggagcgc gatgcaccag catcccgcca tggcccagat
     5281 gatccagcgc cgcagtcgcg gactagtcgg ccgcggatgg cgggcggtat tgcccgaagg
     5341 cgtcctggta ctcgtcagca acctcgattg cggacttgtg gacgtagatt cgggttgttt
     5401 gcggtgaggc gtgtccaagg attgtctgta ccgtcgattc gcgaacccgg cgccggacca
     5461 tctccgtggc gagtccgtgc cggaaagagt gtggcgtgat ttcgctttcg atgccaagcg
     5521 tcgtgcagag ctggctgatg atgtgctcaa ccgtccgcgg gcttatcgca ttgccggcat
     5581 cgccgacctt gtctcgcccg ctgaacgccg gcgcagcgcc ggcgccgcga atcgcgtcgc
     5641 cccgcaagtt ccagtacgcc tggagcgcct gggccgtttc ggcgtcgaaa tggacggttc
     5701 ggctcttgcc gcccttggcc cggtagacat gcgcctgacc gtcagccagc tcgatgtcgc
     5761 gacgacggaa cgcgcagagc tccgagacgc ggacaccggt ccggaagagc atcaggatca
     5821 tcgcccgcac tttgaacgcc cggagctgct tgacctcgtt ctcgtcccgc tccggctggc
     5881 ggacgaactc gacgatccgt tcgaggtcgc tcagcttgac gtccggcggc ggcggcgtaa
     5941 agcgcggcat catggcggcg atccgcaccc gcagccgatc ggtcgaaagg ctcggatgga
     6001 ggtcgtacgc agccaggtac gagcagaact tgcgcacggc cgagaggtta ttctgcgccg
     6061 tccgcatctg cgaaacctct tccggcgagc gcacatcggg cggcacgatc agcgaggcga
     6121 aggaggtgac gtgctcggcc gagatgatcg tgaccggcgc gacgtccgga tcgatcccct
     6181 cgtgatcggc caggtgccgg acgaacttgg caatgccgtt ccggtaggtc ttcttcgagc
     6241 gcggggcgag gcgaagatcg ccgaagaaga tctcgaccgc cgccgtgatc gtcagcggac
     6301 cgttcgtttg tgcagcaacg gagtcgttgt cgatagccag ccccggaaat gtatcgagtt
     6361 catcgtgttc gatagccaac aggtacctcc actgatgctc agcgcatcag ccagccaaaa
     6421 ggaaccctac gggaatcggg gagtacgtac actctaggac gtggcgccgg gagcgtcaag
     6481 gctcactcgt cgtcctcagc gacccgagcg tagcgctgga tcagggctgc ggcgtcgcga
     6541 tggaactcgc tgttggcgac gatccgggtc aggccggctt gcttggccgc gcgccgcgtc
     6601 tcgacatctg tgtgcgagcc aaagccgagt gaggggatcg ctggatagtc ggccaggatt
     6661 ccgcccagcg tctcccagtc gaccccctcg ccgagcacat tcatgtcgat gatcacgagg
     6721 gcggccgtct ggtggtgctc atggagggcg cgcccgaccg catcgggatt cgccgccagg
     6781 accaggtcga acccgagctc cttggccaag ttgcgcaggc gcaccccgaa gaagacatcc
     6841 cgattgaggg cgatcagatg gagcggatcc tgcggcagcg aggttcgata catgcgggaa
     6901 gtgtccaatg tga
//
LOCUS       DATAOZ010000480         6614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3090146, whole genome shotgun sequence.
ACCESSION   DATAOZ010000480 DATAOZ010000000
VERSION     DATAOZ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3090146"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..327)
                     /locus_tag="VHR64_12315"
     CDS             complement(43..327)
                     /locus_tag="VHR64_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303660.1"
                     /translation="MSVTLPPEIEVQVRSLVETGEFPDEESAVREAFRLLDLQRRQNR
                     LRSKIAEAETEFDRGEYVEWDDETKAALWEEAIRAAEQDESYDPDVIPPA"
     gene            472..1254
                     /locus_tag="VHR64_12320"
     CDS             472..1254
                     /locus_tag="VHR64_12320"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3303661.1"
                     /translation="MSESAPAIELRDVSATYVERGNRLPVLDGVSLAVAPGEFVAIVG
                     PSGSGKSTLLDVIAGLIEPDAGTVAINGADLPAANRLGKSAYLRQRDLLMPWRTVQAN
                     AAVALEAHGVPRREARSRALDRLRELDLHGFAHAYPAQLSGGMRQRVAFVRTMLTDAP
                     ILLLDEPFAALDALTRSDLHRWLQDVLPGGQRTVLLVTHDVEEALLLADRVLVVSPRP
                     ARVVAVERIAFARPRSRALTLAPEFLAAKARLLIHLGTAAPA"
     gene            1251..2018
                     /locus_tag="VHR64_12325"
     CDS             1251..2018
                     /locus_tag="VHR64_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303662.1"
                     /translation="MSNRWVTQIVAPLLVLAAGIAFWQWRVDVNDTPSWFLPSPRLIG
                     QTLIDDRGLLADNAWVTLREVLIGFAIALVAGVLLAVLIHVSEVLERAIYPIVIASQA
                     IPLVALAPLLLVWFGYGLTPKVIVTSLVAFFPITVGAVDGLRSADRETLNLLRTYGAS
                     RVRRFWMVNVPSALPSLFSGARIGISIAVIGAVFGEYVGANSGLGYLMNISAARLQTE
                     RVFAAIVILAVMAITLFTLVALLERRVLRWRRYVTEE"
     gene            complement(2113..2754)
                     /locus_tag="VHR64_12330"
     CDS             complement(2113..2754)
                     /locus_tag="VHR64_12330"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3303663.1"
                     /translation="MLIEKGDSVKASVISVVPMSADQLDAATGVMARAFQNDPLFKAI
                     WPDSDERASSLSQLARWDLADAGPGDMALVTAGPVNGVAKAIRSDVHAPLTENDFHRL
                     GHALTPHAWQRFNDVHAFSFAVQQLMTKTPEAHWYLQVLAVSPEYQGRGIGSALLDAI
                     HAGADADGLPTTLLTFQPRNVPLYQRNGFQVVAEDTEPGDGLYFWCMKREPRK"
     gene            complement(2757..3386)
                     /locus_tag="VHR64_12335"
     CDS             complement(2757..3386)
                     /locus_tag="VHR64_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3303664.1"
                     /translation="MSGSSIRIVPMRADQQERAAEIFARAFLGDPAMVAAFPDPMERS
                     RVMRVMGDWNIRLGMTFGEVFVTAGQLDGVLTLFSSEREEELEDNIADTYGSMPDEMG
                     PGAWERNQAMGPLWEIPHEALKASVAEPHWYLDLLAVDPGRQGIGIGSALLQAVNARS
                     DADGRPCALFTFTERNAGLYQRHGYEIIAEGEAPIMPLHYWCLKRPPAS"
     gene            complement(3588..4967)
                     /locus_tag="VHR64_12340"
     CDS             complement(3588..4967)
                     /locus_tag="VHR64_12340"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX3303665.1"
                     /translation="MPETTLDLREAILGPTPTKEQILDRIEGEQIRFINLQFSDVMGI
                     VKSVTITAKQFEHVVEGGQWIDGSSIAGFARIAESDMYLIPDLSTFAPIPWETGEYAT
                     ARVICWVFNPNGDPFPGDPRWVLYRQLERLAKKGYLYQTGPELEFFLFSTNGNGISPL
                     PYDQAGYFDYSTDIASTIRKDMVIALDQMGIEVETSHHECAVGQHEIDFMYGHALPTA
                     DRAVTFKYVLKAVAQRHDLHATFMPKPLEGVAGNGMHVHQSFKDIETGQNAFVDPEDP
                     YGLSPIAKQLIGGQLYHARALCAVIAPLVNSYRRLVPGYEAPVYVSWARTNRSALIRV
                     PAIRGGHTAATRVELRCPDPSCNPYLAFAAMLATGLHGIEENLPLPEPVEENLYHFTD
                     DDLKRRNIPMLPATLGEALDEMEKDEVIRDALGDHVFDRLIEAQRSEWADFRRHVSQW
                     ERDRYLAIY"
     gene            complement(5285..>6614)
                     /gene="glnA"
                     /locus_tag="VHR64_12345"
     CDS             complement(5285..>6614)
                     /gene="glnA"
                     /locus_tag="VHR64_12345"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX3303666.1"
                     /translation="HTSKSISDVDEEIFEHGVGFDGSSIRGFQSIDASDMLMMPDPTT
                     ARLDPYTAVPTMYVVCDLYDPTTRKRYDRDPRGIAQRAEAHLKTTGIGDTAFMGPEPE
                     FFVFDNVRFDITNNASFHTVDSIEGYWNSGSENGHGNHGYTTRPKAGYFPVAPHDTLQ
                     DIRTEMVLTLEELGIPVEVHHHEVGTAGQCEIDMRFDTLLAIADKVMTYKYVIRNTAR
                     KHGKVATFMPKPIFADNGSGMHVHQSIWKDGQNLFSDPNGYAGIGETGMHYIGGLLKH
                     GPALMALAAPTVNSYRRLVPGYEAPVNLVYSARNRSAAVRIPTYSCNPKSKRLEFRCA
                     DTAANPYLLFSALLMAGLDGIKNRIDPGDPAEFDLFEADKETLARVKTVPGSLRDVLK
                     SLEDDHEFLLEGEVFSKGFIENWIDYKLENEVEPANLRISPYEFYLYFDV"
BASE COUNT         1110 a         2121 c         2133 g         1250 t
ORIGIN      
        1 atctgccgaa tatcccgccg tgctgcctgg acaacaacaa gcctatgcgg gcggaatgac
       61 atctggatcg tacgactcgt cctgctcggc tgcccggatt gcctcttccc agagcgccgc
      121 cttggtttcg tcatcccact cgacgtactc gccccggtcg aactcggtct ccgcctcggc
      181 aatcttcgat ctcaaccggt tctggcggcg ctgcaggtct agcagccgga atgcctcgcg
      241 caccgcgctc tcctcgtccg gaaattctcc agtctcgacc agagaccgaa cctggacttc
      301 tatctcgggg ggcaaagtga cgctcatgtt gcacactcct cattggagat taccagaatc
      361 gcgaccccga gtaggtcaat acgcgggtct ttgctctccg gactttcgtt tcccctactc
      421 cctcaacccc gtctgctcgc ctggccgcct gctcgcctcc atacttccgg catgagcgaa
      481 tccgccccgg caatcgagct gcgtgatgtc tcggcgacgt atgtcgagcg gggcaatcgg
      541 ctgccggtgc tggatggcgt ctcgctggcg gtggcgccgg gcgagttcgt cgcgatcgtc
      601 gggccgtccg gctccggcaa gagcacacta ctggacgtga tcgccggcct gatcgagccg
      661 gacgcaggca cagtcgccat caacggcgcc gatttgcccg ccgccaatcg tctcggcaag
      721 agcgcctacc tccgccagcg tgatctgctc atgccatggc gcaccgtcca ggcgaacgcc
      781 gccgtcgccc tcgaggcgca cggggtcccg cgccgcgagg ccagatcgcg tgccctcgac
      841 cgtctccgcg agctcgacct gcacggcttc gcccatgcct acccggccca actctcgggt
      901 gggatgcggc agcgagtcgc cttcgtgcgc acgatgctga ccgatgcgcc gatcctgctc
      961 ctggatgaac cgttcgccgc actcgatgcc ctgactcgct ccgatctcca ccgctggttg
     1021 caggacgtcc tgcccggcgg ccaacgaacc gtccttctcg tgacgcacga cgtcgaggag
     1081 gcgctgctgc tggcagatcg ggtgctggtc gtcagcccgc gacccgcccg ggtcgtcgcc
     1141 gtggagcgaa tcgccttcgc ccgaccgcgc agccgggctt tgaccctcgc gcccgagttc
     1201 ctggccgcca aggctcgcct cctgatccat ctcggcacgg ccgcgccggc atgagcaacc
     1261 gctgggtgac gcagatcgtt gcgccgctcc tggtcctcgc ggccgggatt gccttctggc
     1321 aatggcgagt cgacgtgaac gacacgccat cgtggttcct gccttcgccg cgactgatcg
     1381 ggcagacgct gatcgacgac cgcggtctgc tggccgacaa cgcctgggtg acgctgcgag
     1441 aggtgcttat cggcttcgcg atcgcgctcg tcgccggggt cctgctcgcg gtcctgatcc
     1501 acgtctcgga ggtcctcgag cgggcgatct acccgatcgt gatcgccagc caggcgattc
     1561 ccttggtcgc gctcgcgccg ctgctgctgg tctggttcgg gtacggcctg acgccgaagg
     1621 tgatcgtgac gtcgctggtc gccttcttcc cgatcacggt cggcgcggtc gatggcctgc
     1681 gctcggccga ccgggagacg ctcaacctgc tgcggaccta cggcgcatca cgggtgcggc
     1741 gcttctggat ggtcaatgtg ccgtccgcgt tgccgtcgct cttttccggt gcacggatcg
     1801 gcatctcgat tgcggtgatc ggcgcggtct tcggcgagta cgtcggcgcg aactccggtc
     1861 tcggctacct gatgaacatc tcggctgcca gattgcagac cgagcgggtc ttcgccgcga
     1921 tcgtgatcct ggcggtcatg gcaatcaccc tcttcactct cgtcgccctc ctcgagcgcc
     1981 gcgtattgcg gtggcggcgc tatgtgacgg aggagtaagc gagctggtgg atggtggtta
     2041 gtggttggtg gatggatacg gaaagggagc cagtagccgg aaatcagtac gctcgttccc
     2101 gaaaacccga acctacttcc tcggttcgcg cttcatgcac cagaagtaca aaccatcgcc
     2161 tggctcggtg tcttcggcaa cgacctgaaa gccgttgcgc tggtagagcg gcacatttcg
     2221 tggctggaac gtgaggagcg tggttggtaa gccatccgcg tcagcgccgg catgaatggc
     2281 atctagcaga gcgcttccga tgccgcgccc ctggtattcg ggcgagaccg cgaggacctg
     2341 aaggtaccag tgcgcttcag gtgtcttcgt catcaattgc tggaccgcga agctaaaggc
     2401 gtgaacgtca ttaaaccgtt gccatgcgtg cggcgtcagc gcatgcccca ggcgatgaaa
     2461 gtcattctcg gtcaggggtg cgtgaacatc gcttcgaatg gctttggcca cgccgttgac
     2521 cgggccggcc gtgacgagcg ccatgtcgcc gggacccgcg tcagccaaat cccagcgtgc
     2581 aagttggctc aatgaggagg ctcgttcgtc ggagtctggc cagatcgcct tgaacagggg
     2641 atcgttctga aatgcacggg ccatgacgcc ggtggcagca tcaagctggt cggcggacat
     2701 gggaacaacg gagattactg acgctttcac gctatccccc ttctcgatga gcaagcctag
     2761 ctcgccggtg gacgcttcag gcaccagtag tggagcggca tgattggggc ttcgccttcg
     2821 gcgatgatct cgtacccatg tcgctggtaa agcccggcat ttcgttcggt gaaggtgaac
     2881 agggcgcagg gccggccgtc ggcgtcggag cgcgcattca cggcctgcaa gagcgcgctc
     2941 ccgatgccga tgccctgccg gccaggatcg accgcgagca aatcgaggta ccagtgtggc
     3001 tcggccacag acgccttcag cgcctcgtgc gggatttccc agagcggccc catcgcctgg
     3061 ttccgctccc aggccccggg tcccatctca tccggcattg agccgtaggt atcagcgatg
     3121 ttgtcctcca gctcctcttc ccgctcgctc gagaaaagtg tcagcacgcc atcgagctgg
     3181 cccgcggtga cgaacacctc gccgaacgtc atacccagac ggatgttcca gtctcccatc
     3241 acccgcatta ctcgtgagcg ctccatggga tccgggaacg ccgcaaccat cgccggatca
     3301 ccgaggaagg cacgggcaaa gatctccgcc gcgcgctcct gttgatcggc gcgcatcgga
     3361 acaatgcgga ttgacgatcc tgacatggcg atcatcgctt gactgaccag agatcaatgg
     3421 tccgaccgta acacggcgaa caacaaagtc aacaacacct cgtagggcag taaccctcac
     3481 ctccgacccc gctccagcca agaaaaaggg cgggagcggg gagattgagt ccccgtcccg
     3541 ccgtgaactt cgcgccttcg cgccttcgcg cctccgcgac tccgcgccta gtagatcgcc
     3601 aagtagcgat cccgctccca ctgggagacg tggcggcgga agtcggccca ctcgctgcgc
     3661 tgcgcctcaa tcaggcggtc gaagacgtgg tcgccgagcg cgtcgcggat gacctcatcc
     3721 ttctccatct cgtcgagcgc ctcgccgagc gtggccggca gcatcggaat gttgcgccgc
     3781 ttcaggtcgt cgtccgtgaa gtggtagagg ttctcctcga ccggctccgg cagcggcaga
     3841 ttctcctcga tcccgtgcag gccggtcgcc agcatcgcgg cgaaggccag gtacgggttg
     3901 caggagggat ccgggcagcg cagctcgacc cgcgtcgccg ccgtgtgccc gccccggatg
     3961 gccgggaccc ggatcagcgc cgagcggttc gtccgcgccc acgagacgta aaccggcgcc
     4021 tcataccccg ggaccagccg gcggtaggaa ttgaccagcg gcgcgatcac cgcgcagagc
     4081 gcgcgggcgt ggtagagctg cccgccgatc aactgcttgg cgatcggtga gaggccgtac
     4141 ggatcctcgg gatcgacgaa ggcgttctgg ccggtctcga tgtccttgaa gctctgatgg
     4201 acgtgcatgc cgttgccggc gacgccctcg agcggcttcg gcatgaaggt ggcgtgcagg
     4261 tcgtgccgct gggcgaccgc cttgagcacg tacttgaagg tcacggcccg gtcggcggtc
     4321 ggcagcgcgt gcccgtacat gaagtcgatc tcgtgctggc cgacggcaca ctcgtgatgg
     4381 ctggtctcga cttcgatgcc catctgatca agcgcgatca ccatgtcctt gcggatggtg
     4441 gaggcgatgt cggtcgagta gtcaaagtag ccggcctgat cgtagggcag gggcgaaatg
     4501 ccgttgccgt tcgtggagaa gaggaagaac tcgagctccg gtccggtctg gtagaggtag
     4561 cccttcttgg ccagccgctc gagctgccgg tagaggaccc agcgcggatc gcccgggaag
     4621 ggatcgccgt tcgggttgaa gacccagcag atcacccgcg ccgtcgcgta ctcgcccgtc
     4681 tcccacggga tcggcgcgaa ggtcgagagg tccgggatca ggtacatgtc ggactcggcg
     4741 atgcgggcaa aaccggcgat cgaggagcca tcgatccact gcccgccttc gacgacgtgc
     4801 tcgaactgct tggcggtgat agtcacgctc ttgacgatgc ccatcacgtc cgagaactgg
     4861 aggttgatga aacggatctg ctcgccttcg attcgatcca gaatctgctc tttggtcggg
     4921 gtcgggccga ggatggcctc ccgcaggtca agggtggtct caggcacgca ggtgctcctc
     4981 tcaaaaaaga cgtggcagga aaaccctgcc acgtcgccgg tgccgacaat tttaacggct
     5041 aaccattccg caggacgagg ctgatcatcg cgacgaagag gggtcccgac ttcgaaccgg
     5101 tcgtccgtcg ccgcccgggg ataaatgccc gggctgagaa acggtaagtc cactaaaggg
     5161 actgaaggaa agcaactcct caaagggccg ccggttcata gccccttcag tgggcttctc
     5221 actggtcagc ccgcgccttt agccgcgggc agttgagacg gattctcgaa tcgaggagag
     5281 gactctacac gtcgaagtac aggtagaact cgtacgggct gatccgcagg ttggccggct
     5341 cgacctcgtt ctcgagcttg tagtcgatcc agttctcgat gaagcccttg ctgaagacct
     5401 ctccctcgag caggaactcg tggtcgtcct ccagtgactt gagcacatcg cggagcgacc
     5461 ccggcacggt cttgaccctg gccagcgtct ccttgtcggc ctcgaacagg tcgaactcgg
     5521 ccggatcgcc cgggtcaatc ctgttcttga tgccgtcaag accggccatc agcagcgcgc
     5581 tgaagagcag gtacgggttc gcggcggtgt cggcacagcg gaactcgagc cgcttgctct
     5641 tcgggttgca ggaataggtc gggatccgga ccgcggctga gcggttccgc gccgaataga
     5701 caaggtttac gggtgcttcg tagcccggca cgaggcggcg atagctgttg accgtcggcg
     5761 cggccaaggc catcagggcc ggtccgtgct tgagcaggcc gccgatgtag tgcatgccgg
     5821 tctcaccgat gcccgcatag ccgttgggat cggagaagag gttctggccg tccttccaga
     5881 tcgactggtg gacatgcatg cccgagccgt tgtcggcgaa gatcggcttc ggcatgaagg
     5941 tcgcgacctt gccgtgcttc ctggccgtgt tgcggatgac atacttgtag gtcatcacct
     6001 tgtctgcgat cgcgagcagc gtgtcgaagc gcatgtcgat ctcgcactgc ccggccgtgc
     6061 cgacctcgtg gtggtgaacc tcgaccggga ttcccagctc ctcgagcgtc agaaccatct
     6121 cggtgcggat gtcctgcagc gtgtcatgcg gggcgaccgg gaaatagccg gccttgggcc
     6181 gagtcgtata gccgtggttg ccgtgtccgt tctcgctgcc gctgttccaa tacccctcga
     6241 tcgagtcgac cgtgtggaag ctggcgttgt tggtgatgtc gaagcggacg ttgtcgaaga
     6301 cgaagaactc cggctccggg cccatgaacg ccgtgtcgcc gatgccggtg gtcttgaggt
     6361 gtgcctcggc ccgctgcgcg atgccgcgcg gatcgcggtc gtagcgcttg cgggtagtcg
     6421 gatcgtaaag gtcacagacg acgtacatgg tcggaacggc ggtgtagggg tcgaggcggg
     6481 cggtggtcgg gtccggcatc atcagcatgt cggaggcgtc gatcgactgg aagccgcgga
     6541 tcgaggagcc atcgaagccg acaccgtgct cgaagatctc ctcatcgacg tcggagatcg
     6601 acttcgaggt gtgc
//
LOCUS       DATAOZ010000481         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5719419, whole genome shotgun sequence.
ACCESSION   DATAOZ010000481 DATAOZ010000000
VERSION     DATAOZ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5719419"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VHR64_12350"
     CDS             complement(<1..431)
                     /locus_tag="VHR64_12350"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3303667.1"
                     /translation="MAHWLLFTCGYPFSGKSTLSAAIGDQFDFEVVSVDAQFGANNVE
                     TWLDAYLAAYRAIDVGLDAGRSVVFDSVGHTRKHRDRLRRKAMRFRAEPLAIWLCVSI
                     PEADQRRQANAVAPVRAHVPDEGFDEIVRAFEPLQSDEAHL"
     gene            complement(432..1265)
                     /locus_tag="VHR64_12355"
     CDS             complement(432..1265)
                     /locus_tag="VHR64_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HEX3303668.1"
                     /translation="MYVIKIGGGAAIDEAAYARFADDLALLHEAGKPFVVVHGGNAEF
                     SRLSAALGMPPRMITNDKGRVSRYTDRETIDAMLMTYCGKVNKTIVATFQQAGINAVG
                     LSAIDGRIATGRRKPVLRGTEDGKPKVLRDDHAGSIETIDTALISLLLANGYVPVLTP
                     PALAVEEGRPINVDGDKLALNLARDLSADALLFFSDTPGLLADKDDESTLIREIDASD
                     PANALASARGRMVVKVEQALKAIEHGVGQVIFGDARVEYPVQRALAGEGTRIGVPVTS
                     Q"
     gene            complement(1274..2320)
                     /gene="argC"
                     /locus_tag="VHR64_12360"
     CDS             complement(1274..2320)
                     /gene="argC"
                     /locus_tag="VHR64_12360"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX3303669.1"
                     /translation="MVRVSIIGGSGYAGGEAVRLLLDHPEAELQQVTSERNAGKFVRA
                     LHPNLRKRTDMKFVSIDKLESCDVLFVALPHGVSMGKMACLREKAPIIIDLSADFRLN
                     DPAGYPTWYDHAHSDPGMLDDFVYGVPELHREEIRRGNLIAAGGCMATTSILGLYPLF
                     RAGIVDLERPVVIECKTGSSGSGGAPGLGSHHPERSGAMRSFKPTGHRHSAEIIQELT
                     FDGRMPEIAFSATSIEAVRGILATSHVYLKDDLSDKEVWAIYRAAYKDEPFMRVVKES
                     SGVYRYPEPKILSGSNYCDVGFERDPHSKRLVVMAATDNLMKGAAGQAVQCFNVRMGC
                     EEDLGLEFPGLHPI"
     gene            complement(2386..>3084)
                     /gene="lysX"
                     /locus_tag="VHR64_12365"
     CDS             complement(2386..>3084)
                     /gene="lysX"
                     /locus_tag="VHR64_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEX3303670.1"
                     /translation="RALYTLRVLNDWGIPTVNTYQVASTCGDKLLTSSALAQAGVPIP
                     RTILAYDHDSALAAIEEIGYPVVMKPAIGSWGRLLSRINDRDAAEAILEHKTTLGSFH
                     HGAFYIQEYVNKPGRDIRSFVVGDETICAIYRDSPHWITNTARGGVATNCPVTPEINE
                     LSLRSARAVGGGVVAIDLLESENGILVNEVNYTMEFRNSIDTTGVNIPARIVDYVIEV
                     GMAAHERRPEIVAA"
BASE COUNT          544 a         1020 c          940 g          580 t
ORIGIN      
        1 atcagatgcg cctcatccga ttgaagtggt tcgaatgccc tgacgatctc gtcgaatcct
       61 tcatccggca catgagcgcg gaccggggcc accgcgttcg cctgccggcg ctggtcggcc
      121 tcggggatcg atacacagag ccagatcgcc aatggctcgg ctcggaatcg catcgccttg
      181 cggcgtagcc gatcccggtg tttgcgcgta tggcccaccg aatcgaagac gaccgatcta
      241 cctgcgtcga gtccgacgtc aatcgcccgg taggcggcca ggtacgcatc cagccaggtc
      301 tcgacgttgt tagcaccaaa ctgcgcgtca actgagacga cctcgaagtc gaattgatcg
      361 ccgatggcgg cactcagcgt cgacttgccg ctgaagggat aaccgcacgt gaacaacagc
      421 caatgcgcca cctattggct cgtcaccggc acgccgattc gggtcccttc accggccagc
      481 gcgcgctgta ccggatactc gacccgcgca tcgccgaaga tgacctgccc aacgccgtgc
      541 tcgatcgcct tgagcgcctg ctcgaccttg accaccatcc gacccctggc cgatgccagc
      601 gcatttgccg gatcggacgc gtcgatctcc cggatcagcg tcgactcatc gtccttgtcg
      661 gcaagcaggc ccggcgtatc cgagaagaag agcagcgcat ccgccgacaa atcccgcgcc
      721 aggttgagcg ccaacttgtc gccgtcgacg ttgatcggtc gcccctcttc gaccgccagc
      781 gccggcggcg tcaggaccgg cacgtatccg ttcgcaagca gcaacgagat cagcgctgtg
      841 tcaatggttt cgattgagcc ggcgtgatcg tcgcgcagca ctttcggttt gccgtcttcg
      901 gtaccgcgca ggaccggctt gcgccgaccg gtcgcgattc ggccatcgat cgcggagaga
      961 cccactgcgt tgattcctgc ctgctgaaac gttgcgacga tggtcttgtt gaccttgccg
     1021 cagtaggtca tcagcatcgc atcgatcgtc tcgcggtcgg tgtaccggct gacgcggccc
     1081 ttgtcgttgg tgatcatgcg cggcggcatc ccgagcgcgg cgctcaaccg gctgaactcc
     1141 gcattcccgc catggaccac cacgaacggt ttacctgcct cgtgcagcaa ggccagatca
     1201 tcagcgaacc tggcatacgc ggcctcatca atcgccgccc caccaccaat cttgatgacg
     1261 tacatcgttc ctcctagatc gggtgcaggc cggggaattc gaggccgagg tcttcttcgc
     1321 agcccatgcg aacattgaaa cactggactg cctggccagc tgcgcccttc atcaggttgt
     1381 cggtcgcggc catcacgacc agccgcttcg agtgcggatc gcgctcgaag ccgacgtcgc
     1441 agtagttgct gcccgagaga atcttcggct ccgggtagcg atagacgcca ctcgattcct
     1501 tgacgactcg catgaacggc tcgtccttgt aggcggcgcg atagatcgcc cagacctcct
     1561 tgtctgacag atcatccttc agatagacgt gggaggtcgc gaggatgccc cggaccgcct
     1621 cgatcgaggt cgcagagaat gcgatctccg gcatgcggcc gtcgaaggtc agctcctgga
     1681 tgatctcggc gctgtggcgg tggccagtcg gcttgaacga gcgcatcgcc ccgctccgct
     1741 cgggatggtg cgagccaagt cccggcgcgc cgcccgaacc ggatgagccg gtcttgcatt
     1801 cgatgacgac cggccgctcc agatcgacga ttccagcccg gaagagcgga tagagaccga
     1861 gaatcgaggt cgtggccatg cagcctccgg ccgcgatcag gttgccgcgc cggatctcct
     1921 cccggtgcaa ctcggggacg ccgtagacga agtcgtcgag catccccggg tcgctgtgcg
     1981 cgtggtcgta ccaggtcggg tagccggccg ggtcgttgag gcggaagtct gcgctgaggt
     2041 cgatgatgat cggcgccttc tcgcgcaggc aggccatctt gcccatcgag acgccatgcg
     2101 gcagcgccac gaagaggaca tcgcacgact cgagcttgtc gatcgagacg aacttcatgt
     2161 ccgtccgctt gcgcagattc ggatggagcg cgcggacgaa tttgcctgca ttgcgctcgc
     2221 tcgtgacctg ctgcagctcc gcttccgggt gatcgagaag gaggcgcact gcctctccac
     2281 ccgcataccc ggacccgcca atgatcgata cccgaaccat gatccccttc tgtcccttcc
     2341 gatgcctcgt gaggaggcgc gggcgcattg caatgcgccc ctacattacg ctgcgacgat
     2401 ttcgggtcgg cgctcgtgtg cggccatgcc gacttcgatg acgtaatcga cgatccgggc
     2461 cgggatattg accccggtgg tgtcgatgct gttccggaac tccatggtgt agttgacttc
     2521 gttcaccagg atcccgttct cactttcgag caggtcaatc gcaaccactc cgccgccgac
     2581 cgcccgcgcc gatcgcagcg agagctcgtt gatctcgggc gtgaccgggc agttggtcgc
     2641 gacgccgccc cgtgccgtgt tcgtgatcca gtgcggcgag tcccggtaga tcgcgcaaat
     2701 cgtctcgtct ccgacgacga atgagcgaat gtcccggccc ggcttgttga cgtactcctg
     2761 gatatagaac gcgccgtggt ggaacgagcc gagcgtcgtc ttgtgctcga gaatcgcctc
     2821 agccgcgtcg cgatcattga tccgcgaaag caaccggccc cacgagccga tcgccggctt
     2881 cataacgacc gggtagccga tctcctcgat cgccgccagc gccgaatcgt ggtcataggc
     2941 gaggatcgtc cgcggtatcg gcacgcccgc ctgagcaagt gccgacgagg tcagcaactt
     3001 atcaccacag gtgctcgcga cctggtaggt gttgacggtc gggatgcccc agtcgttcag
     3061 cacccgtagg gtatagagcg cgcg
//
LOCUS       DATAOZ010000482         6692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4402174, whole genome shotgun sequence.
ACCESSION   DATAOZ010000482 DATAOZ010000000
VERSION     DATAOZ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4402174"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..406
                     /gene="rplO"
                     /locus_tag="VHR64_12370"
                     /pseudo
     CDS             1..406
                     /gene="rplO"
                     /locus_tag="VHR64_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            552..1862
                     /gene="secY"
                     /locus_tag="VHR64_12375"
     CDS             552..1862
                     /gene="secY"
                     /locus_tag="VHR64_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX3303671.1"
                     /translation="MLEAVLNAFKIPDLRRKLLFTLGMLVIFRIFASIPVPGVNQKEL
                     QDFIEQNQLLSVLNLFSGAGLSNFSVVALGVYPYITASIIMQLMTPIIPRLNELSMEG
                     QQGRNKINQYTHWLTVPLAFLQAYGHAILFTRSQSGGVSLLEGFGLFDKSTFIPSLAI
                     LLSMTAGTMLLTWMGELITEMGIGNGTSIIIFGGIVAHLPSTLGRLFGGGASRDIIGV
                     VTFAAIALITVVGIVLINEGQRRIPVQHAKRIRGNRMYGGTTTFIPLRVNSAGMIPLI
                     FAVSIMVFPGMIANFLSSSSREGVRSFAGWLSRFFNPQSFQYNLIYFLMVVGFTYFYT
                     MVVFQQQRIPENLQRQGAFIRGVRPGRQTALYLQRVLNRITLVGALFLGAIAVLPYFA
                     SQLTGINNLLLSATSLLIVVGVAIDTMRQLEAQLMMRNYEGFIS"
     gene            1874..2569
                     /locus_tag="VHR64_12380"
     CDS             1874..2569
                     /locus_tag="VHR64_12380"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX3303672.1"
                     /translation="MSSGHHVILMGAQGAGKGTQAERLAPALNLYHLSTGEAFRSAIS
                     AQTELGVLAKGYLDRGDLVPDDVTLGIVADKLEEIASGAGPERKDGALFDGFPRTPSQ
                     AEGLTDQLENRGEHIAAVIEIAVPRDDLIERLSGRRICPTCGTVYHVTFNPPKVAGIC
                     DKDGTPLIQREDDTPEAIARRLNLYFEMTAPVLAYYRNLGLLETVDGRKSIDEVQADI
                     RAIVEARMAATRK"
     gene            2575..3342
                     /gene="map"
                     /locus_tag="VHR64_12385"
     CDS             2575..3342
                     /gene="map"
                     /locus_tag="VHR64_12385"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX3303673.1"
                     /translation="MAITIKNEREIGLMREASQAVALVHARIEAAIAPGVTTKDLDDI
                     ARDTLQEVGAVSLFLGHLGFKGRICASVNEEIVHGIPGKRVLQDGDIISVDVGARVGG
                     YCGDSAWTYPVGNISDEARHLLEATEKSLYLGIEQAKVGAHLNAIGHAVEEYAESQGL
                     GLVREYGGHGIGREMWEDPHVPNHGSPDSGPRLRAGMTLAIEPMLNIGGDETLQLADG
                     WTVITADRTLSAHFEHTIVIREDGPEILTKRLSGVVK"
     gene            3446..3559
                     /gene="rpmJ"
                     /locus_tag="VHR64_12390"
     CDS             3446..3559
                     /gene="rpmJ"
                     /locus_tag="VHR64_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX3303674.1"
                     /translation="MKVAASVVRRCDRCKIVRRKGILRVICSNPKHKQRQG"
     gene            3563..3934
                     /gene="rpsM"
                     /locus_tag="VHR64_12395"
     CDS             3563..3934
                     /gene="rpsM"
                     /locus_tag="VHR64_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX3303675.1"
                     /translation="MARISGIDLPREKRVEVGLTYIYGIGLHTSQKLLQRTGINPETR
                     VRDLTEDEVQRLRDIIDKELRVEGDLRREVSMNIKRLMDIGCYRGLRHRRGMPVRGQR
                     TRTNARTRRGPKRAIGLKKKK"
     gene            3998..4414
                     /gene="rpsK"
                     /locus_tag="VHR64_12400"
     CDS             3998..4414
                     /gene="rpsK"
                     /locus_tag="VHR64_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX3303676.1"
                     /translation="MSERRRGATQTKGKTRVRRRDRKNVPRGRAYIQATFNNTIVTLT
                     DPTGNVLSWSSSGANGFKGSRKSTPFAAQVTAEQAARRAMEHGLRTVDVFVKGPGSGR
                     EMAIRALQSAGVNVISITDTTPIPHNGCRPPKRRRV"
     gene            4452..5090
                     /gene="rpsD"
                     /locus_tag="VHR64_12405"
     CDS             4452..5090
                     /gene="rpsD"
                     /locus_tag="VHR64_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX3303677.1"
                     /translation="MARYTGPVCRLCRREGMKLYLKGARCDTPKCAVSKTRKNYPPGQ
                     HGQRRTRRQSEYGLQLREKQRVRRYYGILETQFRRLYEEAVRRGGVTGENLLQLLESR
                     LDNVVYRMGFADSRAQARQLVRHGHFTVNGRKTNIPSFIVKPGDVVTVKAEKRSKTYF
                     EDFNVQLETKRPPDWVSVNVNDLSGKVLSLPTRDQMELPPFNEALIVEYYSR"
     gene            5131..6228
                     /locus_tag="VHR64_12410"
     CDS             5131..6228
                     /locus_tag="VHR64_12410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX3303678.1"
                     /translation="MFEKPQFRIDTEETGLNYGRYKIEPLEPGYGTTLGNALRRILLR
                     SLEGVAISRVQIEGVWHEFSTINNVREDVVEIVLNLKRVRLKRVTEFSGETRAHLYVR
                     ADGSGERVVTAGDIAWPSEVEIVNPDQVIATLTGADAILDMDLWIARDRGYRPAEHQE
                     AYSIGQIPIDAIFTPVQKVNFVVEHTRVGQVTDFDRLILEILTDGTIEPEEALSEAAR
                     VLMDHARIFADFNRKETETAVTQGAFISEEVQNKALADLGLSPRVLNALRSRQIEKVG
                     QVLTMDPDQLLSIRNFGPRSLKELRDKLSEYGYLPEGDAGIFSADHGYFDALESAMDG
                     DEDDESRDVAEAIASLAGDDLGMSDTDGEEA"
     gene            6231..6584
                     /gene="rplQ"
                     /locus_tag="VHR64_12415"
     CDS             6231..6584
                     /gene="rplQ"
                     /locus_tag="VHR64_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX3303679.1"
                     /translation="MRHRKAGRKFGRNPAQRRALLRQLAVSMILHERITTTEAKAKTI
                     RPIVEKLVTIAREDSHAHRKLIMSKIDHPLATAKLFEVIAPRFIETPGGYTRIAKLEP
                     RRGDAAPMALIEFIA"
BASE COUNT         1349 a         2016 c         2125 g         1202 t
ORIGIN      
        1 gaagaagggc aagaagcgcg tcggtcgagg caccggcagc ggccacggca agacatcggg
       61 ccggggtacc aagggccaga agtcgcgctc gggtccgggc gttcgacgcg gattcgaggg
      121 cggccagctc ccgatcatga agcgaatgcc gtacaagcgg ggcttcacga acatcttccg
      181 cgccgagtgg gaagtcgtga acctcgacac catcgctgag catggcctga gcggcgagat
      241 cacgccggag acactctacg agcgcggtgt gactcgcggg ctcgagttcc cggtcaaggt
      301 cctcggaaac ggcgaggctg ccgccaagct gtcgatcagc gcccacgctt tctcggagag
      361 cgccaaggcc gcgatcgagg ctgctggcgg ctccgtaacc gtgctcgagc ggaccgatcg
      421 atgggtgacc gcccggccac gcagccggcg cttgccaatc aaccgcgagc tgaaggaagc
      481 ccgcttcggg aaggttggcg gtccgcagcg ccgagacgaa gtctccaact aggaggcagt
      541 aaggacctgg tatgctcgag gccgttctaa acgcattcaa gattcccgat ctgcggcgca
      601 aactgctgtt cacgctcggg atgctggtga tcttccgcat tttcgctagc atcccggtgc
      661 ccggcgtgaa tcagaaagag ctgcaggact tcatcgagca aaaccagctc ctgagcgtcc
      721 tcaacctgtt ctctggagcg ggcttgagca acttctccgt ggtggcgctg ggcgtctatc
      781 cctacatcac cgcgtcgatc atcatgcagc tcatgacacc gatcattccc cggctgaatg
      841 agctgtcaat ggaaggccag caaggccgca acaagatcaa ccagtacaca cactggttga
      901 ccgtgcccct ggcctttcta caggcctatg gtcacgccat ccttttcacg cgcagccaat
      961 cgggcggcgt cagcttgctc gagggcttcg gcctcttcga caagagcacc ttcattccgt
     1021 cgcttgcgat cctgctgtcg atgaccgccg gcacgatgct cctgacctgg atgggtgagt
     1081 tgatcaccga gatgggcatc ggtaacggaa cgtcgatcat catcttcggc ggcatcgtgg
     1141 cgcacctgcc cagcacgctc ggccggctct ttggcggcgg ggcatcgaga gacatcatcg
     1201 gcgtcgtcac ctttgccgcc atcgcgctca tcacggtggt cggcatcgtc ctgatcaacg
     1261 aggggcagcg ccgaatcccg gttcagcacg caaagcggat ccgtggcaac cggatgtatg
     1321 gcggcacgac cacgttcatt ccgcttcggg tcaactcggc gggcatgatc ccgctgattt
     1381 tcgcggtcag catcatggtg ttcccgggca tgatcgccaa cttcctgagc tcgtcgagcc
     1441 gggaaggcgt tcggagcttt gctggatggc tctcgcggtt tttcaacccg cagagcttcc
     1501 agtacaacct gatttacttc ctgatggtgg tcggtttcac gtacttctac acgatggtcg
     1561 tgttccagca gcagcggatc cctgagaacc tgcagcgcca gggagccttc atccggggcg
     1621 tgcgaccggg caggcaaaca gcgctgtatc tccagcgcgt gctgaaccgc atcacgcttg
     1681 tgggcgcgct cttccttggc gcaattgcag tcctcccgta cttcgcgtcg cagcttaccg
     1741 ggatcaataa tcttcttctc agcgcgactt cattgctgat cgtcgtcggc gtagcgatcg
     1801 acaccatgcg ccagctcgaa gcccaactga tgatgcggaa ttacgaaggc ttcattagtt
     1861 aggggatcgt ttcgtgagct ctggacatca tgtcatcctg atgggcgcgc agggcgccgg
     1921 caagggaacc caggccgaac ggctggctcc cgcgctgaac ctgtaccacc tgtcgaccgg
     1981 cgaagcgttt cgctccgcaa ttagcgcaca gacggagctc ggcgttctgg cgaagggata
     2041 cctcgatcgg ggcgaccttg ttcccgacga cgtcacgctc gggatcgtcg ccgacaagct
     2101 ggaggagatc gccagtggcg ctgggccaga acgcaaggat ggcgcgctat tcgatggttt
     2161 cccgcgcacg ccctcccagg ccgagggcct gacggatcag ctcgagaatc gtggcgagca
     2221 tattgccgcg gtgatcgaga tagccgtgcc acgcgatgac ctgatcgagc gcctgtcagg
     2281 ccgacggatt tgcccgacat gcgggacggt ctatcacgtc acgttcaacc cgcccaaggt
     2341 cgctggaata tgcgacaagg acggaacacc gctgatccag cgcgaggacg acacaccgga
     2401 ggcgattgca cgtcgcctga atctctattt cgagatgacg gcgccggtcc tcgcctatta
     2461 ccgtaacctc ggcctgctcg aaacggtcga cggccgcaag tccatcgacg aggttcaggc
     2521 cgacattcgc gcgatcgtcg aggcccgcat ggccgcgaca cgcaagtaag caagatggcg
     2581 ataacgatca agaacgaacg cgagattggt ctgatgcgcg aggccagcca ggccgtcgcc
     2641 ctcgtccatg ctcgcattga ggcggcgatt gcgcccggtg tcacgaccaa ggatctggac
     2701 gacatcgccc gcgacacgtt gcaggaagtc ggggcagttt ccctgttcct tggccacctg
     2761 ggcttcaagg gacgaatctg cgcgtccgtg aacgaggaga tcgttcatgg cattccgggg
     2821 aagcgcgtcc tccaagatgg cgacatcatc tctgtggatg tcggcgctcg cgtcggtgga
     2881 tattgcggag actccgcctg gacctatccg gtcggcaata tcagcgatga ggctcgccat
     2941 ctgcttgagg cgactgaaaa gtcgctctat ctcggtatcg agcaggcaaa agtcggagcg
     3001 catctcaatg cgatcggaca cgcggtcgag gagtatgcgg aaagccaggg actcggactg
     3061 gtccgcgagt atggcggtca cggaatcggc cgggagatgt gggaggaccc gcatgttccg
     3121 aaccatgggt cgccggattc cggcccgcga ctgcgtgccg gcatgaccct ggcgatcgag
     3181 ccaatgctga acatcggtgg ggacgagacg ctccagctcg cggatggctg gacggtgatt
     3241 acggccgacc gaacgctctc agcgcacttc gagcatacga tcgtcattcg cgaggatggt
     3301 ccagaaatct tgacgaaacg gctgtccgga gtggtaaaat aggactcttg tgcgcatgcc
     3361 ggaggcgtgc gcctttacgt gtgcgtaaaa ctgccgttcc ccgcgtccct ggcgatggag
     3421 aacaaggcaa ggggaatttg gtcagatgaa ggttgcggca tcagtagttc ggcgatgcga
     3481 tcgatgcaaa atcgttcgcc gcaagggcat tttgcgcgtc atttgcagca acccgaagca
     3541 caagcagcgg cagggataag agatggcacg tatttcagga attgaccttc cacgagagaa
     3601 gcgcgtagag gtaggcctca cctatatcta cggcatcgga ttgcatacca gccagaagct
     3661 cctccagcga actggcatca atcccgagac acgggtgcgg gacctgacgg aagatgaagt
     3721 ccagcggctg cgcgacatca tcgacaaaga gctccgtgtc gagggagatc tccggcgcga
     3781 ggtctcgatg aacatcaagc gattgatgga catcgggtgc tatcgcggcc ttcgccatcg
     3841 gcgaggcatg ccggtgaggg gtcagaggac ccgcacaaat gcgcgtaccc ggcgcggtcc
     3901 gaagcgggca attggtctga agaagaagaa gtaggcaggt ttggatccgc attcgacttg
     3961 attgcggatc cggattgggc agagaggtac accgcgaatg tcagagcggc ggcgaggagc
     4021 cacccagaca aaaggcaaga cacgcgtacg gcgtcgagat cggaagaatg ttccccgagg
     4081 tcgggcgtac atccaggcaa cgttcaacaa cacgatcgtt accctgaccg atccgacggg
     4141 gaacgtgctg tcctggtcga gctccggagc caatggcttc aaggggtccc gcaagagcac
     4201 cccgttcgcg gcgcaggtca cggccgagca ggctgctcgc cgggccatgg agcacggact
     4261 tcgcaccgtc gacgtcttcg tcaagggtcc gggttccggg cgcgagatgg cgatccgggc
     4321 gcttcagtct gccggtgtca atgtgatctc gatcaccgac accacgccca ttccgcataa
     4381 cggttgtcgt ccgccgaagc ggcgtcgcgt ctagccggca ctgccgcatt cgaggaggaa
     4441 tctgagtcgt tatggcgaga tatacgggcc cagtgtgccg cttgtgccgg cgcgagggca
     4501 tgaagctcta tctgaagggc gcacggtgcg atacaccgaa gtgcgcggtc agcaagaccc
     4561 ggaagaacta tcccccgggc cagcatggcc aacgccgcac ccggcggcag tcggagtacg
     4621 gcttgcagct gcgggagaag cagcgcgtgc ggcggtatta cggcatcctg gagacccagt
     4681 ttcggcgtct ctacgaggaa gcggtccgcc gcggcggcgt gactggtgag aacctgctcc
     4741 agctcctgga gagccgcctc gataacgtcg tctaccgcat gggatttgcc gacagccggg
     4801 cgcaggcgcg acagctcgtc cggcatgggc acttcacggt aaatggccgc aagaccaata
     4861 ttccgtcgtt catcgtcaag ccgggcgatg tggtcacggt caaggctgag aagcggtcca
     4921 agacctattt tgaagacttc aatgtgcagc tcgagaccaa gcgcccgccg gactgggtga
     4981 gcgtgaacgt caacgatctg agcggcaagg ttctgagcct gccgactcgc gaccagatgg
     5041 agctgccgcc gttcaacgag gcgctgatcg ttgagtacta cagccggtaa tctccggcgc
     5101 atagctcaat aagcaaggag gcgatctcgc ttgttcgaga aaccgcaatt ccgaattgac
     5161 acagaagaga ccggtctcaa ttacggccga tacaagattg agccgctcga gccgggctat
     5221 ggcacgacgc tgggcaatgc gctgcgccga atcctgctgc gctcccttga gggcgtcgcg
     5281 atctcgcgcg tccagatcga aggtgtttgg cacgagttct cgacgatcaa caacgtccgc
     5341 gaggatgtcg tcgagatcgt cctgaacctg aagcgtgtcc gcctcaagcg ggttaccgag
     5401 ttcagcggag agacccgcgc gcacctctac gttcgggccg acggctcggg cgagcgcgtt
     5461 gtgactgccg gcgacatcgc ctggccgtcc gaagtcgaaa tcgtcaaccc ggatcaggtc
     5521 atcgcgaccc tgaccggcgc tgatgccatc ctcgacatgg atctctggat cgcgcgcgac
     5581 cgcggctatc gcccagccga gcaccaggag gcctactcga tcggtcagat cccgatcgac
     5641 gcgatcttca cgcccgtcca gaaggtcaac ttcgtggtcg agcacactcg cgttggccag
     5701 gtgacggact tcgatcggct gattctcgag atcctgaccg acggcaccat cgagcccgaa
     5761 gaggccctca gcgaggcagc ccgcgtgctc atggaccacg cccggatctt cgcggacttc
     5821 aaccgcaagg agacggagac ggccgttacc cagggcgcct tcatctcgga agaggtccag
     5881 aacaaggcgc tggctgatct tggcctgtcg ccgcgggtgc tcaatgcgct tcgcagccga
     5941 cagatcgaga aggtcggcca ggtcctgacg atggacccgg accaactcct ctcgatccgc
     6001 aactttggtc cgcgctcgct caaggagctg cgggacaagt tgagcgagta cggatatctg
     6061 ccggagggcg atgccggtat cttctcggct gatcatggct actttgacgc gctcgagtcc
     6121 gccatggatg gcgacgaaga cgatgagagc cgcgacgtcg cagaagcgat tgcatcgctc
     6181 gccggtgacg atctcggcat gagtgacaca gatggtgagg aagcgtagcg atgcgacacc
     6241 gtaaggcggg ccgcaagttc ggccgaaacc cagcccaacg acgggcactg cttcggcaat
     6301 tggcggtgtc gatgatcctc cacgagcgga tcactacgac cgaggccaag gccaagacca
     6361 ttcggccgat cgtcgagaag cttgtgacga tcgcgcgcga ggattcgcac gcgcatcgca
     6421 agctgatcat gtcgaagatc gaccacccgc tggcgaccgc caagctgttc gaggtgatcg
     6481 cgccgcgctt catcgagacg ccgggcggat acacccgtat cgcgaagctc gagccgcgcc
     6541 gcggcgatgc cgcgccgatg gcattgatcg agttcatcgc ctgagttgat tgacgagtcg
     6601 acgcgggaag cggtgcggcc caggctgaag ctggttctcg cgtacgacgg caccgagtta
     6661 gctggctcgc aacgccagag cggcaatcgc ac
//
LOCUS       DATAOZ010000483         7304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5459160, whole genome shotgun sequence.
ACCESSION   DATAOZ010000483 DATAOZ010000000
VERSION     DATAOZ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5459160"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..797
                     /locus_tag="VHR64_12420"
     CDS             135..797
                     /locus_tag="VHR64_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303680.1"
                     /translation="MQQSVGRRRVIAGGAALLGGAALRHGAAAQDQLYVASGTGVNLK
                     MMMDGDGQPTVPLRELFGFDSYYAQCVIEDNAERFSMDTFEMGKVVVEPHTFFMAMYA
                     NEISLVSIQDMGNGATMAKLTGDLGCLTEVGTATGKVGSRESKEPAFFEIEAVDGGHG
                     GGTVGDKFVFTVYFDPKKAPVNHGIFGPKPTFTGEMIAGEVTIQPVAGKPLLAPSGTP
                     VS"
     gene            839..2266
                     /locus_tag="VHR64_12425"
     CDS             839..2266
                     /locus_tag="VHR64_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3303681.1"
                     /translation="MAHVLNRGSLSRRAVLGGAVALGALGIGRSNARAATSSAPETWR
                     TWLLISPDELRPSAPGNPPQSELDEVIALQSQRSVDSGIKISTWGDGPAVLPWTGIAL
                     DLIQVHRPGPVRAARALALLHAALADTVIAVRDAAAAYPRPAPSQADAQIVPLGSGNA
                     AGSSFPSEHAAVASCAAAVLAYLFPKEPTDHLTTLATEAAESRLWAGAAYRSDIVAGQ
                     AIGQAVGTRAVDRGMADGSDHAWDGSGRPTADGVWQPTPPEYVQTPLDPLAGTWTPWL
                     LPSGDAYRPAAPPAYGSAAWQAELAGVQEAVARRTSDQETLVKQWAGGGGTVTPAGLW
                     IQIARDLIVRDDLDSPLAARALALTSVAMADGFICCWDAKYAYWTERPITADPTLDVL
                     IPTPPFPSFTSGHATASTSAATILGHLFPADAADLLGKAAEASQTRLWAGIHFPIDCD
                     VGTVGGGMVGRLSILRAQSDGAEAG"
     gene            complement(2334..2861)
                     /locus_tag="VHR64_12430"
     CDS             complement(2334..2861)
                     /locus_tag="VHR64_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3303682.1"
                     /translation="MSESSIPAGARIGHIHLKVGNLDRQIGFYRDFLGFDLLMRFGDQ
                     AAFLSAGGYHHHIGMNTWESKDGDPPPPGATGLYHFAINYPERRDLAAALKRLYDHGW
                     QIDGASDHGTHEALYLHDPELNGLELAWDRDPSEWIRDGKIVFDRTSLDFADLLNELN
                     DPSTEQLLPALAAYT"
     gene            complement(2959..3441)
                     /locus_tag="VHR64_12435"
     CDS             complement(2959..3441)
                     /locus_tag="VHR64_12435"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3303683.1"
                     /translation="MGMTKDERAALIEQFGAGYDAVLDALEGITAAEWESREGPNEWC
                     TSEIVHHLGDSEMSAAVRIRALIADEDPVIQAFDQDRWNDVLYPRDRPVEPSLAAFKA
                     AREATAPLLSLMTDDQWTREGRHTAFGPMSAEYWLEIYGPHAHGHADQIRRARAAAHG
                     "
     gene            3580..5127
                     /locus_tag="VHR64_12440"
     CDS             3580..5127
                     /locus_tag="VHR64_12440"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX3303684.1"
                     /translation="MTEPAASLLPSHETAQPVVELRDVTKQFGAVQALRGVHLALYPG
                     EVHALVGENGAGKSTLVKMLGGIHQPDAGTILLNGQEMTLGSPLDARAHGIAVIQQHP
                     FLFPDLDVAENVYMGRQPKSRFGRIDWREMNREVDVLLRPLGVSFTATTPVRGLSVAD
                     QQMVEIAKALSQNASVLIMDEPTASLSAREVERLFSIVRRLREQGVAILFVSHRLDEV
                     ATLSDRVTIFRDGGYVITAPTSELSTDQIIRHMVGRRLDSLFPKEVAEIGEVALSVRE
                     LSRLGEFHDVSFELRRGEIVGLFGLVGAGRTEVARVIFGVDRAERGMIQLNGRSITIA
                     DPTDALEHGIAYVPEDRHGQGLIMSFPIDENITLPILRRLSRFTLVKSDEAEKIAADY
                     STRLQVKSAGLDQLVSALSGGNQQKVVLGKWLATEPHVLILDEPTRGIDIGTKAEVHR
                     IISHLAAQGLAILMISSELPEILGMSDRVMVMHEGRISGEFDRSEADQERIMFAATGQ
                     VAAGIPG"
     gene            5124..6119
                     /locus_tag="VHR64_12445"
     CDS             5124..6119
                     /locus_tag="VHR64_12445"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303685.1"
                     /translation="MTSGTQLVTILGRIREAGLVAALTAIIVGVTISEPRFTSVNNIR
                     QILLSVSILAIVAIGQTLVVLTRNIDLSVASIVGLVAYEVRDILAHNPQMSIGLAIVI
                     GLLIGASLGAVNGALVTVGRVPAIVATLGTLYVFRGLTFALGGGKLITASQEPPDFKR
                     IALSRPWGIPAPIIIAAVLGVVFAYLLRYSRVGRQLYAIGSNPDAARPAGIRTNRVVF
                     GAYVMCGLLCGVGGVLWGSRFGSIDARAASGMELQVVAAVVLGGVNIFGGSGTIVGAM
                     LGAVLLGTIQNALTILRLNQFWIQAISGAAILLAVTIDLLITRRLRRSLTLRRAA"
     gene            6116..7117
                     /locus_tag="VHR64_12450"
     CDS             6116..7117
                     /locus_tag="VHR64_12450"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303686.1"
                     /translation="MSRRFWVSWEALLLAMLLALIAIGWATSDVFGQGSTITLITTNS
                     MEKALMALPMTLIIISGEIDLSVASTLGLSSAVIGKTWAAGWPLWLCIATVLAVGAIC
                     GLFNGLLVTKLGLPSLVVTLGTLALYRGLAYVILGSEAISNYPDRFASFGFKEIPGTR
                     VPWTMLVFIPLLIVLLILLHRSWIGRQIYAAGSNAEAARFAAVRVNQLKLGLFVLSGV
                     VAAIAGVMFTARVSSSRADNATGFELDVVAAVLLGGVSIFGGRGTLIGVVLSLGVIGT
                     LRQVLALNSSNAELQSIAIGSLLILSVLGPNLARSAAGLRTGRQFAGMRSRGATPAD"
BASE COUNT         1147 a         2367 c         2453 g         1337 t
ORIGIN      
        1 agccggcagg gtcccgtagc cggaaccgtt tcgggaactc cgggggcaac gccggcgccc
       61 gcgcccggtg tagtcgatgc cgtcttcgac ctgcttctgc ctggacaggg gtaggggctg
      121 ccatgaactc acgggtgcaa cagtcagtcg gccggcgaag agtaatcgcg ggaggggcgg
      181 ccctgctggg cggggccgct ctgcggcatg gagccgcggc ccaggatcag ctctacgtgg
      241 cgtcaggcac cggggtgaac ctcaagatga tgatggatgg ggacgggcag ccgacggtgc
      301 cgctccgcga gttgttcggc ttcgactcct actacgccca gtgcgtgatc gaagacaacg
      361 ctgaacgctt ctccatggat accttcgaga tggggaaggt ggtggtcgag ccgcacacct
      421 tcttcatggc gatgtacgcc aacgagatca gcctcgtcag catccaggac atgggtaacg
      481 gggcaacgat ggccaagttg acgggtgatc tcggttgcct gacggaggtc ggcaccgcaa
      541 ccggcaaggt cggttcgcgg gagtcgaaag agccggcgtt cttcgagatc gaagcagtcg
      601 atggtggtca cggtggaggt accgtcggcg acaagttcgt gttcactgtc tacttcgatc
      661 ccaagaaggc acccgtcaat cacggaatct tcggccccaa gccgactttc accggggaga
      721 tgatcgccgg tgaagtgaca atccagcccg tggccggtaa gcctttgctt gccccatcgg
      781 gcacgccggt gtcgtaagcg cttgccgtct ccggcgagcg tattcccgga ggaatccgat
      841 ggctcacgtg ctcaatcgcg gttccctgag ccggcgtgcc gttctgggtg gtgctgttgc
      901 ccttggcgcg ctcggcatcg gccgatccaa tgcgcgggcg gcgacctcat ccgctccaga
      961 gacctggcgg acctggctgc tgatctcccc ggacgagctg cgtccgtctg ccccgggcaa
     1021 tccgccccaa tccgagctgg acgaagtaat cgcgctgcaa tcgcagcgtt ccgtcgattc
     1081 tggcatcaag atctcgacct ggggtgacgg tccggctgtg ctgccgtgga ctggcatcgc
     1141 actcgatctg atccaggtgc atcggccggg tccggttcgc gcggcccgcg cgctggcgct
     1201 gctgcacgcg gcgctggccg acacggtcat cgccgttcgc gacgcggccg cggcctatcc
     1261 gcgcccagcc ccctcccagg ccgacgccca gatcgtgcca ctgggcagtg gcaacgcagc
     1321 cggatcgtca ttcccgtcgg aacatgcggc ggtggcatcc tgcgccgccg cggtgctggc
     1381 ctacctgttc ccgaaggagc caaccgatca cctgaccacg ctcgcgactg aggcggctga
     1441 gtcccgcctg tgggccggcg ccgcctaccg gagtgacatc gtggccgggc aggcaatcgg
     1501 acaggcggtc ggcacgcgcg cggtggatcg gggtatggcc gatggatcgg atcatgcctg
     1561 ggatggcagt ggccggccaa ccgccgacgg cgtctggcag ccgaccccac ccgaatacgt
     1621 gcagacgccg ctcgacccgc tggccggcac ctggacgccg tggctcttgc cgagcgggga
     1681 cgcctatcgc cccgccgcgc cgcccgccta tgggtcggcg gcatggcagg ccgagctcgc
     1741 cggcgtacag gaggcggttg cccggcggac atcggaccag gagacgctgg tcaagcaatg
     1801 ggccggcggt ggggggacag tcacgccggc gggcctctgg atccagatcg cccgcgatct
     1861 gatcgttcgc gacgatctcg attcaccgct ggccgcacgg gcactggctc tgaccagcgt
     1921 cgccatggcc gatggcttca tctgctgctg ggacgcaaag tacgcctact ggacggagcg
     1981 cccgatcacc gccgatccga cgctggacgt tctgattccg acgccgccgt ttccgtcgtt
     2041 cacctcaggt cacgcgacgg cgtcgacatc ggccgcgacc atcctcggtc atctcttccc
     2101 ggccgacgca gccgacttgc tgggcaaagc cgccgaggca tcgcagacgc gactctgggc
     2161 cgggatccac ttcccgatcg attgtgacgt cggcacggtc ggcggcggca tggtcggacg
     2221 cctctccatc ctgcgcgccc aatcagacgg agccgaggcg ggttgagggt gaagggtgga
     2281 gggttgaggg aacgaaaagg gcgacgtttt ttcccgaacc ccgaaccccg aggctacgtg
     2341 tacgcagcca gagcgggtaa cagttgctcc gtgcttgggt cgttgagctc attgaggaga
     2401 tcggcgaaat cgagcgatgt gcggtcgaag acgatcttgc cgtcccggat ccactcgctc
     2461 gggtcgcgat cccaggccag ttcgagcccg ttcaactccg gatcgtgcag gtagagcgcc
     2521 tcgtgcgtgc cgtgatcgga cgcgccgtcg atctgccatc cgtggtcgta gagtcgcttg
     2581 agcgcggccg ccagatcgcg ccgctccggg tagttgatcg cgaagtggta gaggccggtg
     2641 gcgccgggcg gtggtggatc gccgtccttg ctctcccacg tgttcatgcc gatgtgatgg
     2701 tgatagcctc cggccgagag gaaggcggcc tgatcgccga atcgcatcag caggtcaaag
     2761 ccgaggaagt cgcggtagaa gccgatctgg cgatcgagat tgccgacctt gaggtggatg
     2821 tgcccaatcc gggcgccggc cgggatagac gattcactca tggctatctc ctttcagcag
     2881 cgatcatagg cgagcaggcg agcagtcgga aaacggaaaa cccgcgactt tcctccaccc
     2941 tccaccctca acccgcactc agccatgagc ggcggcgcgg gcgcggcgga tctggtccgc
     3001 gtggccgtgc gcgtgcgggc cgtaaatttc cagccagtac tcggcggaca tcgggccgaa
     3061 cgcggtgtgc cgaccctccc gcgtccattg atcgtctgtc atcaggctga gcaagggagc
     3121 agtcgcctcg cgcgcggcct tgaacgcggc cagggacggc tcgaccggcc gatcccttgg
     3181 gtagagcacg tcattccagc ggtcctgatc gaaggcctgg atgaccggat cctcgtccgc
     3241 gatcagtgcc cggatgcgga ccgcggcgct catctcactg tcgccgaggt gatggacgat
     3301 ctcgctcgtg caccactcgt tcggtccttc gcgcgattcc cactcggcgg cggtaatacc
     3361 ctcgagcgca tccagcacgg cgtcataccc tgccccgaac tgctcgatca gcgccgcgcg
     3421 ctcgtccttc gtcatgccca tttcgcttgt cctctcgtgt agagtgccgc cagcaacaca
     3481 tgaagcatat ccctctgggc actcccatgt ggctccggcg gagctgaata tgcggtaggc
     3541 tggtcactta ctcacacggc tgtgcggaga cgagcagtta tgaccgagcc agcggcatct
     3601 ctcctgcctt cacatgaaac ggcccagccg gttgtcgagc tgcgtgacgt gaccaagcag
     3661 ttcggcgcgg tccaggcgct gcgcggcgtt cacctcgcac tctatcccgg cgaggtccac
     3721 gcgctggtcg gtgagaatgg ggccggtaag agcacgctgg tcaagatgct cggcgggatc
     3781 caccagcccg acgccggcac gatcctgctc aacggccagg aaatgacgct cggtagtcca
     3841 ctcgacgccc gcgcccacgg tattgccgtc atccagcaac acccgttcct cttccccgac
     3901 ctcgacgtcg ccgagaacgt ctacatgggc cggcagccga agtcgcgctt cggacggatc
     3961 gactggcgcg agatgaaccg cgaagtcgat gtcctgctgc ggccgctcgg cgtctcgttc
     4021 accgcgacga ccccggtgcg cggcctctcg gtggccgacc agcagatggt cgagatcgcc
     4081 aaggcccttt cccagaatgc gagcgttctg atcatggatg agccgactgc ctcgttatca
     4141 gctcgtgagg tggagcggct cttctcgatc gtccggcgac tgcgcgagca aggcgtcgcg
     4201 atcctgttcg tctcgcaccg gctcgatgaa gtggccacgt tgtccgaccg ggtcacgatc
     4261 tttcgggacg gcggatacgt catcaccgcg ccgaccagcg aactctccac cgaccagatc
     4321 atccgccaca tggtcggccg ccggctcgat tcgctcttcc cgaaggaggt cgccgagatc
     4381 ggcgaggtcg cgctctcggt gcgcgagctg agccggttgg gcgagtttca cgatgtttcg
     4441 ttcgagttgc ggcgcggcga gatcgtcggc ctgttcgggc tcgtcggcgc cggacggacc
     4501 gaggtcgccc gggtcatttt cggcgtcgac cgggccgagc ggggcatgat ccagctcaac
     4561 ggccggtcga tcacgatcgc ggacccgaca gatgcgctcg aacacggcat cgcctacgtc
     4621 ccggaggatc gccatgggca ggggctgatc atgagcttcc cgatcgacga gaacatcacc
     4681 ctgccgatcc tgcgccggct ctcccggttc acgctggtca agtccgatga ggctgaaaag
     4741 atcgcggccg actactcgac gcggctgcag gtcaagtcgg ccgggctgga tcagctcgtc
     4801 tcggcgctct cgggcggcaa ccagcagaag gtcgtgctcg gcaagtggct ggcgaccgaa
     4861 ccgcacgtcc tgatcctcga cgagccgacc cggggcatcg atatcggcac caaggccgag
     4921 gttcaccgga tcatctcgca cctcgccgcg caggggctgg cgatcctgat gatttccagc
     4981 gagttaccgg agattttggg catgtccgac cgggtgatgg tcatgcacga ggggcggatc
     5041 agcggcgagt tcgatcgcag cgaggcggat caggagcgaa tcatgtttgc ggcaaccggg
     5101 caggtcgcgg cggggattcc gggatgacca gtggtacgca actggtcacg atcctcgggc
     5161 ggatccgtga ggccggtctc gtcgcggcgc tgacggcgat catcgtcggc gtcacgatca
     5221 gcgagccgcg attcaccagc gtcaacaaca tccgccagat cctgctctcg gtctcgatcc
     5281 tggcgatcgt cgcgatcggc caaacgctgg tcgtgttgac ccgcaacatc gacctctcgg
     5341 tcgcttcgat cgtcgggctg gtcgcctacg aggtgcggga catcctcgcc cacaatccgc
     5401 agatgtcaat cgggctggca atcgtgatcg ggctgctgat cggcgcgagc ctgggcgcgg
     5461 tcaacggcgc gctggtcacc gtggggcggg ttccggcgat cgtggcgaca ctgggcacgc
     5521 tttacgtttt tcgcgggctg accttcgcgc tcggcggcgg caagctgatc acggcctcgc
     5581 aggagccgcc cgacttcaag cggatcgccc tctcgcggcc gtggggcatc ccggcgccga
     5641 tcatcattgc cgccgtgctc ggcgtggtgt tcgcctacct gcttcgctat tcgcgggtcg
     5701 gccgccagct ctacgcgatc ggtagcaacc cggacgcggc ccgaccagcc gggatccgga
     5761 caaaccgggt tgtcttcggc gcgtacgtga tgtgcggtct gctctgcggg gtcggcggcg
     5821 tgctctgggg atcgcgcttc ggctcgatcg acgcccgcgc ggccagcggc atggagctgc
     5881 aggttgtggc ggcggtcgtc ctcggcgggg tcaatatctt cggcggctcg ggcacgatcg
     5941 tcggggcgat gctgggcgcg gtgctgctcg gaacgatcca aaatgcgctg acgatcctgc
     6001 gcctgaacca gttctggatc caggccatct ccggcgcggc gatcctgctg gcggtgacga
     6061 tcgacctgct gatcacccgc cgtctgcgcc gctccctgac gctgaggcgc gcggcatgag
     6121 ccggcggttc tgggtgagct gggaggccct gctcctcgcg atgctgctgg cgttgatcgc
     6181 gatcggctgg gcgacatcgg acgtcttcgg ccagggctcc acgatcacat tgatcacgac
     6241 caacagcatg gaaaaggcgc tgatggcgct gccaatgacg ctgatcatca tcagcggcga
     6301 gatcgatctc tcggtggcgt ccacgctcgg gctctcgagc gcggtaattg gcaagacctg
     6361 ggcggcgggc tggccgctct ggctctgcat cgcgaccgtg ctcgccgtcg gggcgatttg
     6421 cggcctgttc aacggtttgt tggtcacgaa gctcgggttg ccttcgctcg tcgtcacgct
     6481 cggcacgctg gcgctctatc gcggcttggc ctatgtgatc ctgggcagcg aggcaatcag
     6541 caattacccg gatcgtttcg cctcgttcgg cttcaaggag attcccggca cgcgggtgcc
     6601 ctggacgatg ctcgtcttca tcccgttgct gatcgtgctc ctgatcctgc tgcatcggag
     6661 ctggattggc cggcaaatct acgcggcagg gagcaatgcc gaggcggcgc gctttgcggc
     6721 ggtccgggtc aaccagctca agctcggcct cttcgtgctg agtggggtcg ttgcggcgat
     6781 cgccggcgtc atgttcacgg cgcgagtctc gagcagccgg gccgacaacg cgaccggctt
     6841 cgagctggac gtcgtagcgg cggttttgct tggtggggtg agcattttcg gcggacgcgg
     6901 tacgctaatc ggcgtcgttc tttcactcgg tgttatcggc acgctccgcc aggtcctcgc
     6961 gctcaattcg tcgaacgcgg agctgcagag catcgccatc ggcagtttgc tgatcctttc
     7021 cgtgttgggt ccgaacctcg cgaggagcgc ggccggactg cgcacgggtc gccagtttgc
     7081 cggcatgcgg tcgcgcgggg ccacacccgc cgactagcca acgaagggag ggcagcgctt
     7141 agcggactcg tattggatcg attgattcag cgtgtcaccc gcaggagggt ctgatgaaga
     7201 accagcgtgt ctttacccgt ttggcaatca ccctgctcct cttgttatcc gcgatctgct
     7261 ccttcggggc gagcagtcat gcactggccc aggccgcgga ctgc
//
LOCUS       DATAOZ010000484         6437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6772629, whole genome shotgun sequence.
ACCESSION   DATAOZ010000484 DATAOZ010000000
VERSION     DATAOZ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6772629"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..575)
                     /locus_tag="VHR64_12455"
     CDS             complement(66..575)
                     /locus_tag="VHR64_12455"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3303687.1"
                     /translation="MIEISEVTALTDQEAGELADILIRIVKSGASIGWVDTPDPAEAR
                     AYWQSVVGKTENVLVLAQENGHVIGTGYLELAQRENGRHRAEVNKVLVHPDRQGQGIG
                     RKLMDALEASARVRGRTLLHLDTDQFDGTNAFYMRCGYTQAGTVPNWARSGQDGTLHG
                     TTFYYKVLE"
     gene            complement(572..1456)
                     /locus_tag="VHR64_12460"
     CDS             complement(572..1456)
                     /locus_tag="VHR64_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX3303688.1"
                     /translation="MSWFTEPMQYEFFRHGLLVAVLVGGLCGLIGTMIVLRRMSYIGH
                     GLSHAVFGGAVVAYLMNVNFYLGAGLWGFLSALLINATGRRRNIGADAAIGIVTTASF
                     ALGVALISRTRSFTRNFDAALFGNILGVTTTDILVISAVALVVVIGTFVFYRQILFTA
                     FDSELAQVFGVPSQWVEVGFALALAATIVASMNIVGVTLIAAAIVIPPSTARLLTHEF
                     GTMLVLSTLIGSACGAIGMFLSYHEDISSGSAIVLLAAGFFALVYAGTTLHHALRPAP
                     KLAAELEPAEQWTAAQNP"
     gene            complement(1453..2262)
                     /locus_tag="VHR64_12465"
     CDS             complement(1453..2262)
                     /locus_tag="VHR64_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3303689.1"
                     /translation="MTRPAAIEVEDLSCGYDRTPVLERVSLSLPAGSFAGVIGPSGAG
                     KTTLLKAILGLATRFSGEVRVQGESIRPSEHPARVGYVPQVETVDWSFPATVNDVVLM
                     GLSVKPGRLPWSSRAERRMIEETLVDLGIGGLGRRHIRDLSGGQQHRVFLARALVGKP
                     DILILDEPTASVDIKTRDDILHHLAHLNQRGTTILMTTHELNSLAAHLPWVVCVNHGI
                     VAQGCPTKVFTDAILSRTFNAEMRVVRDQTTGNLLVAEAGTHGPFADLVAS"
     gene            complement(2259..3299)
                     /locus_tag="VHR64_12470"
     CDS             complement(2259..3299)
                     /locus_tag="VHR64_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX3303690.1"
                     /translation="MSFRTSRRSVLGLAIAALSSPLPKFVSAQEATPIPPTSSLGWSD
                     SFDTSNGLPNVVTTVAPISSIARNVGGTRINLAGVVPDGTNSHTFEPAPSDAVLLSKA
                     DVVFVNGLQLEQPTMDLAEANLKDGAEIDSLGDKTISPDQYVYDFSFPKDEGKPNPHL
                     WMNPVFAANYAALISAKLSERDATNADYYAMNLANYQDLLNRLDQGIMAATATIPEQN
                     RKLLTYHDSYAYFAPRYGLTVIGAAQPSDFSEPSAKDVAQLIDQIKKEHVPAVFGSEV
                     FPSAVLEQIAKEAGAQYIDQLRDDDPPGKPGDPEHTYVGMMLKNMQLMIPALGGNVDA
                     LAGIEPWDTYQP"
     gene            3407..3658
                     /locus_tag="VHR64_12475"
     CDS             3407..3658
                     /locus_tag="VHR64_12475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX3303691.1"
                     /translation="MPRYAYLCGKCGAEFDAERPMAESGEPSLCPVCGHSADRVITPP
                     KFLFKADPRDNRPYWHSHDGYAHSHPPRRGRHRSPEEDH"
     gene            complement(3678..4550)
                     /locus_tag="VHR64_12480"
     CDS             complement(3678..4550)
                     /locus_tag="VHR64_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3303692.1"
                     /translation="MPRPLPAPPVRVRRALSLAGQELPLTQYIFDNAATQAAQRFGSL
                     EALYDQRTLRFLDATGVGTGWRCLEVGAGGGSVAAWLAERAGGTGHVLVTDIDSRYLE
                     ALSALGRPNVEVRHHDIGQDPLPEATFDLIHARLVLIHVPQREVALTRLIAALRPGGW
                     IVIEDFDPTFIESGFPATEQGDYETFDILRVAMRRLMEQRGVDPAWGRSLYRRLVDHG
                     LADVGLKGHFAAWQGASPGALLQRANFEQIRAEAVTAGLVTDEQVDRAVAVLNDPGFS
                     FGSPVMMTAWGQKR"
     gene            complement(4617..5552)
                     /locus_tag="VHR64_12485"
     CDS             complement(4617..5552)
                     /locus_tag="VHR64_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3303693.1"
                     /translation="MTDHPFQSVLVPLDGSALSEQALPFAMAIAGQGGSVILLQAIPD
                     AEPLRKPFGAVTMSAEEVLQMLTNLAEADLDRAEAHWADVAPEVTITKKAVSGDAATV
                     ILDTAAEQNIDLIALASTGRGALGRLTLGSVADRVLRTADRPVLVVRGYDAPSSHQLP
                     QLSRIVVPLDGSDRSMLAIPAAATVAKQMQAPVELLMAVDLPQVVSPAMAYGPAFSPE
                     FYSELEEQTTRNADAYLTEAVAEFGKHGVEAKKHIMLGSAVAAILDFAKHGDLIVMTS
                     RGQGGFKRWILGSVAEKLVRDAPAPVLLVPSHHHE"
     gene            complement(5633..6259)
                     /locus_tag="VHR64_12490"
     CDS             complement(5633..6259)
                     /locus_tag="VHR64_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX3303694.1"
                     /translation="MKTIVCYGDSNTYGADPATGTRHAPDVRWTGVLQAELGSGYRVI
                     EEGLSGRTTNLEDIIELNRNGLTYLAPCLESHAPIDLVTIMLGTNDLKARFNRNASDI
                     AQSAGLLANIARSMRVGPDDGPPRVLLMAPPVVVGLTDLDGMFAGAIEKSKQFAHYYA
                     IWAANNHVGFLDAGSVIVSSPTDGIHIDAPEHAKLGKAVAAKVREIIG"
     gene            complement(6256..>6437)
                     /locus_tag="VHR64_12495"
     CDS             complement(6256..>6437)
                     /locus_tag="VHR64_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303695.1"
                     /translation="WLSKRDCAELMATCIEAEDVPFAVVYAISNNPRQFWDLSHAKEL
                     LGWTPKDSAPVGPDA"
BASE COUNT         1129 a         2194 c         2003 g         1111 t
ORIGIN      
        1 gaacattatc aatccccggc gacatggagc gagcgatacc tgtggcgata cgcgcggcat
       61 cggtgctatt cgagcacctt gtagtagaac gtcgtgccgt ggagggtgcc gtcctggccg
      121 gagcgagccc agttcgggac ggtgccggcc tgggtgtacc cgcagcgcat gtagaaggcg
      181 ttcgttccgt cgaactggtc ggtgtcgagg tgcagcagcg tccggccccg caccctggcc
      241 gacgcctcga gcgcatccat caacttccgt ccgatcccct ggccctgccg atcagggtgg
      301 accagcacct tgttgacctc ggcccggtgc cgcccgttct cgcgctgcgc cagctcaagg
      361 taaccggtac cgattacatg gccgttctcc tgcgcaagga caagcacgtt ctcggtcttg
      421 ccgacgaccg actgccagta cgcccgcgcc tcggctggat ccggcgtgtc gacccagcca
      481 atcgaggcgc ccgacttcac gatccggatc aggatgtcgg ccaattcgcc ggcttcctga
      541 tcggtcaacg cagtcacttc gctgatctcg atcatggatt ctgcgccgcc gtccactgct
      601 cggccggctc aagctccgcg gccagcttcg gcgccggtcg caacgcatgg tgcaatgtcg
      661 tgccggcata gaccagcgcg aagaatccgg ccgccagcag cacgatcgcc gagccagacg
      721 agatgtcctc gtggtagctc aggaacatgc cgatcgcccc gcaggccgat ccgatcagcg
      781 tcgacagcac cagcatcgtc ccgaactcgt gcgtcagcag ccgcgcagtg ctgggcggga
      841 tcacgatcgc cgccgcgatc agcgtcacgc cgacgatgtt catcgaagcc acgatcgtcg
      901 cggccagcgc cagcgcgaag ccgacctcga cccactggga cggcacgccg aagacctgcg
      961 ccagctcgct gtcgaacgcg gtgaagagaa tctgccggta gaagacgaag gtgccgatca
     1021 cgaccaccag cgcgaccgcc gagatcacga ggatgtcggt cgtcgttacg ccgaggatgt
     1081 tgccgaagag cgccgcatcg aagttgcgcg tgaacgaccg cgttcggctg atcagtgcca
     1141 cgccgagcgc gaatgacgcc gtcgtgacga tgccgatggc cgcgtctgct ccgatgtttc
     1201 gccgccggcc ggttgcgtta atcagcagcg ccgagaggaa gccccagagg ccagcgccga
     1261 ggtagaagtt gacgttcatc aggtaagcga cgaccgcgcc accaaacacg gcatgtgaga
     1321 ggccatggcc gatgtagctc atccgcctca gcacgatcat cgttccgatc aggccgcaca
     1381 acccgccgac cagcaccgcg accaacaggc cgtgccggaa gaactcgtac tgcatcggct
     1441 cggtgaacca gctcatgacg ccaccagatc cgcgaaggga ccgtgcgtcc ccgcctcggc
     1501 caccagcaag ttgccggtcg tctggtcccg cacgacccgc atctcggcgt tgaaggtccg
     1561 gctcaggatc gcgtcggtga agaccttcgt cggacacccc tgcgccacga tcccgtgatt
     1621 gacacagacg acccacggca ggtgtgccgc cagcgaattc agctcatgcg tcgtcatcag
     1681 gatcgtcgtc ccgcgctggt tgaggtgtgc gaggtggtgc aggatgtcgt cccgcgtctt
     1741 gatgtcgacg ctcgcggtcg gctcatcgag gatcaggatg tccggcttgc cgaccagcgc
     1801 tcgggccagg aacacccggt gctgttgtcc accggaaaga tcgcggatgt gccgccgccc
     1861 gagtccgccg atgcccagat caaccagcgt ctcctcgatc atccgccgct ccgctcgcga
     1921 gctccacggc agccgacccg gcttgaccga cagccccatc aggaccacgt cgttgaccgt
     1981 cgcgggaaag ctccagtcga ctgtctcgac ctgcggcacg tacccgaccc gcgccggatg
     2041 ctcgcttggc cggatcgact cgccttggac gcgcacctcg ccggagaatc gcgtcgccag
     2101 ccccaggatc gccttgagca gcgttgtctt gccggccccg ctcggcccga tcacgccggc
     2161 gaacgacccc gccggcaacg acagcgacac gcgctcgagg accggcgtcc ggtcgtagcc
     2221 gcagctcaga tcctcgactt cgattgccgc cggtcttgtc atggctggta ggtatcccac
     2281 ggctcgatcc cggccagcgc atcgacattg ccgccgagcg ccgggatcat cagctgcata
     2341 ttcttgagca tcatgcccac ataagtgtgc tcaggatcgc ccggcttgcc cggcggatca
     2401 tcatcgcgga gctggtcgat gtactgcgcg ccggcctcct tcgcgatctg ctccagcacc
     2461 gcgctcggga agacctccga gccgaagacg gctggcacgt gctccttctt gatctggtcg
     2521 ataagttgcg cgacatcctt ggccgacggc tccgagaagt cggacggctg ggccgcgccg
     2581 atcaccgtca aaccgtaacg cggcgcgaaa taggcgtagg agtcgtgata cgtgagcagc
     2641 ttccggttct gctcgggaat cgtcgcggtc gcggccatga tcccctggtc gagccggtta
     2701 agcagatcct ggtagttcgc gaggttcatg gcgtagtagt cggcgttcgt cgcgtcgcgc
     2761 tccgacagct tggctgagat caacgcggcg tagttggccg cgaagaccgg gttcatccag
     2821 aggtgcggat tgggcttgcc ctcgtccttt ggaaacgaga agtcgtagac gtattggtcc
     2881 ggcgagatcg ttttgtcgcc gagcgaatcg atctccgcgc catccttcag gttcgcctcg
     2941 gccagatcca tcgtcggctg ctcgagctgc agcccgttca cgaagaccac gtccgccttc
     3001 gaaagcagca ccgcgtcgga cggcgccggt tcgaaggtgt ggctgttcgt cccatcaggc
     3061 acgactccgg ccaggttgat ccgggtccca ccgacattcc gcgcgatgga gctgattggc
     3121 gcaaccgtcg tgacgacgtt cggcagccca ttgctggtat cgaacgagtc cgaccagccg
     3181 agcgacgacg tgggcgggat cggtgtcgcc tcctgggccg aaacgaactt cggcaacggc
     3241 gacgacaggg cagcgatcgc cagtccaagg actgatcgac ggctggttct gaacgacatg
     3301 tggtctactc catcgggaac tgcgcggcgc agggccgaca ttgcgcgggc cggtaacgat
     3361 tgtactagta gcaaccggtt acagtacgga cgaggacgat gcagagatgc cccggtatgc
     3421 ctatttgtgc ggaaaatgcg gcgccgaatt cgacgccgaa cgccccatgg ccgagtcagg
     3481 agagccgtcg ctctgcccgg tctgcggcca ctccgccgat cgggtgatca ccccgccgaa
     3541 gttcctcttc aaggccgacc cgcgcgacaa ccgcccctac tggcactccc acgatggcta
     3601 cgcccacagc cacccacccc gccgaggccg ccaccgctcg ccagaggaag atcactaaaa
     3661 ctcaccgacc gtccccgcta cctcttttgc ccccaggcgg tcatcatcac gggcgaaccg
     3721 aagctgaagc caggatcgtt gaggactgcg acggcgcgat caacctgttc gtcggtgacc
     3781 agaccggcgg tcaccgcttc tgcccggatt tgctcgaagt ttgcccgctg gaggagcgcg
     3841 ccgggcgagg ccccttgcca ggcggcgaag tgtcctttga ggccgacgtc ggccaggcca
     3901 tgatcgacaa ggcggcggta aaggcttcgt ccccaggctg ggtcgacgcc acgctgctcc
     3961 atgaggcgac gcatcgcgac ccgcaggatg tcgaacgtct cgtagtcgcc ttgttcggtt
     4021 gccgggaagc ctgattcgat gaacgtcggg tcgaagtcct cgatcacgat ccaaccgccg
     4081 gggcgcaagg cggcgatcag gcgagtgagg gcgacctcgc gctgcggcac gtggatcaga
     4141 accaggcgag cgtggatcag gtcgaatgtc gcctcgggca acgggtcctg gccgatatcg
     4201 tgatgtcgga cttccacgtt gggccgaccc agcgcactga gcgcctcaag gtagcgcgaa
     4261 tcgatgtcgg tcacgaggac gtgtccggtg cctccagcgc gttcggctaa ccatgcggcg
     4321 accgaccctc caccagcgcc tacctctagg catcgccaac cggttccgac tcctgttgcg
     4381 tccagaaagc gcagggtgcg ctggtcgtac agcgcctcca ggctaccgaa ccgttgggcc
     4441 gcctgcgtgg cagcgttgtc aaagatgtac tgggtcaatg gcagctcctg accagcgagt
     4501 gatagcgcgc gtcttactcg caccggagga gcggggagcg ggcggggcac ggccgccccg
     4561 cgctgcgtca ggcgatgcgg ggcgtggtaa ccccgcgcta cttcattacg tgggggctac
     4621 tcgtgatggt gcgacgggac cagcaagacc ggcgccgggg catcccgcac cagcttctcg
     4681 gccacgctac ccagaatcca gcgcttgaac ccgccttgac cgcgactggt catcacgatc
     4741 agatctccgt gcttggcaaa gtcgagaatc gccgcgaccg ctgagccgag catgatgtgc
     4801 ttcttcgcct cgacgccgtg cttgccgaac tcggcgaccg cctcggtgag gtacgcatcc
     4861 gcgttgcgcg tggtctgttc ctcgagctcg gaatagaact cgggcgaaaa agccgggccg
     4921 tatgccatcg ccggcgagac cacctgtggc aaatccactg ccatcaatag ctcgaccggc
     4981 gcctgcatct gcttcgcgac ggtcgccgcc gccgggatgg ccagcatcga ccggtccgac
     5041 ccatccagag gcacgacgat ccggctgagc tgcgggagtt ggtgcgaaga tggcgcgtcg
     5101 tacccgcgta ccaccagcac cggccgatcc gccgtccgca ggacacggtc cgccacactg
     5161 cccagcgtca accggcccag cgcgccacgt ccggtgctcg ccagcgcgat caggtcgata
     5221 ttctgctcgg ccgcggtatc gagaatgacc gttgccgcgt caccggaaac cgcctttttg
     5281 gtgatcgtca cctccggcgc gacatctgcc caatgcgcct cggcccggtc cagatcagcc
     5341 tccgccaggt tggtcagcat ctgcaggacc tcctcggccg acatcgtgac cgcgccgaag
     5401 ggcttgcgca gcggctctgc atccgggatc gcctgcaaca aaatgaccga gccgccctgg
     5461 ccggcgatcg ccatcgcgaa cggcagcgcc tgctcgctca gcgccgatcc gtccagcgga
     5521 accagcacgg attggaatgg gtggtcggtc atcacggatc ctccttgcgc aacggaatcg
     5581 acggtcctcc tgcctgcagt gtactgccgc gaccggcgca cattgcgcgg aattacccga
     5641 taatctcgcg gactttcgcc gccaccgcct tgcccagctt ggcatgctcg ggcgcatcga
     5701 tgtggatgcc gtcggtcggg ctgctgacga ttacactgcc cgcatccagg aaccccacat
     5761 ggttgttcgc ggcccagatc gcgtagtaat gggcgaactg cttcgatttc tcgatcgctc
     5821 ccgcaaacat cccgtcgagg tcggttagcc caaccacgac cggcggcgcc atcagcagca
     5881 ctcttggtgg tccatcatcc ggcccgaccc gcatcgaccg cgcgatattg gccagcagac
     5941 cggccgattg ggcaatatcg gacgcgttcc gattgaatct agccttcagg tcgttggtgc
     6001 ccagcatgat cgtgaccagg tcgatcggcg cgtgcgattc gaggcacggc gccaggtagg
     6061 tcaggccgtt ccggttcaac tcgatgatgt cctcgaggtt ggtcgtccgg ccgctcagtc
     6121 cctcctcgat cacccggtag ccggacccga gctcagcctg cagaactccc gtccagcgca
     6181 catccggcgc gtgccgcgtg ccggtcgccg ggtccgctcc ataggtattg gaatcgccgt
     6241 agcagacgat cgtcttcatg cgtcggggcc gaccggagcg ctgtccttcg gagtccagcc
     6301 cagcagctcc ttcgcgtggc taaggtccca gaactgccgc gggttattcg agattgcata
     6361 gacgaccgcg aacggcacat cctccgcctc gatacaagtc gccatcagct ccgcgcagtc
     6421 tcgcttgctg agccagg
//
LOCUS       DATAOZ010000485        15641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1778529, whole genome shotgun sequence.
ACCESSION   DATAOZ010000485 DATAOZ010000000
VERSION     DATAOZ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15641
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778529"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..2803)
                     /locus_tag="VHR64_12500"
     CDS             complement(116..2803)
                     /locus_tag="VHR64_12500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX3303696.1"
                     /translation="MDDRALIDLALARPGEIPEKTLRLLFGHLRRDHRFADARGVLDA
                     LTERTGETGTILDSRADLLESEGRSAEALLTRELRCARYPDSTAWIRLATQLLESDTP
                     DRLDRLAEIDRELGRLPESDLTIEMMRCEIALARDRIDEAQAIASTLLTASTRSSRPP
                     LALARIALSRGDRIGARRHLLDAENRFGTRVAQSDLAKARAEIDRIGDRELATIVDRL
                     VAPPANGIPKTLVAELNEAFAEPEAEPEPVLTAPEPEGTDPRVLDTLRDVFGYDELRP
                     GQAEVIQRCLDGLDTLAIMPTGAGKSLTFQIPALLLDGPVVVVSPLIALMHDQLTTLP
                     EPLRAQSTYINSTLDLNEQERRLAGVRRGEYRLIYAAPERFRQPQFLDAIRTAGIRLV
                     VIDEAHCVSMWGHDFRPDYFFLTRALEELGDPPTLAITATATAEMAHQIGIVLSRSFS
                     MVRTSAFRENLFYSVEKYRDLDAKRERVVELCREASGPTIIYVPSRMHADQLAGRLRY
                     NKIRAVPYHAGMPAADRTRNQNAFIANQAEVIVATIAFGMGINKPDVRMIIHFSPSRS
                     LEAYIQESGRAGRDGQPARCILLYTSGDTGTIRRHASERAVTIDELRDVYRNLRRSAV
                     GRWCIIDRQDLGSFAPDDVDVGVAIGLLEQGGMVRRHPDTGRTMTVRWSHRSAEVDAA
                     QSHDRFGQWLTRIRNGSAIVTVPTATACDELDSNPTVLERAVSQQTGISCAFQGFSTC
                     LELLPTEGTNRGAMDGLLERLAASEKKRAEEMIGYLSTRSCRHVHLAHHLNERLDPCG
                     DVCDTCTTKPSAEQSRRVVPKRRDDPYFEALAQWRREKAREMVVPAFVIASDRLLDRL
                     AADRPRTRADLLRTPGIGVIKANQYGEDILAVIRNVSNN"
     gene            2941..3195
                     /locus_tag="VHR64_12505"
     CDS             2941..3195
                     /locus_tag="VHR64_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303697.1"
                     /translation="MLTTIERMRWATYGLVAGLVVGLFLGWMFHGFVGMIVRLVIIAI
                     ILAPFVVALIFWLKVSNKNRMERTETGIQEAEWHDLNTPG"
     gene            3306..3617
                     /locus_tag="VHR64_12510"
     CDS             3306..3617
                     /locus_tag="VHR64_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX3303698.1"
                     /translation="MPVDFTREDIREGLKQVYDPEIGINIVDLGLVYDADIADNGDVL
                     VTMTLTSLGCPLGPVIVQEVQGALGEFQGIGEIDVKLVWSPPWSPDMMSEDAKDELGI
                     W"
     gene            complement(3779..4588)
                     /locus_tag="VHR64_12515"
     CDS             complement(3779..4588)
                     /locus_tag="VHR64_12515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3303699.1"
                     /translation="MTEQTRTLKRSVIAGSGPDIAVATIGSGPTMILLHGIGSSGMSW
                     MPVMSRLAELYRLIIPDLRGHGQSGQPDHGYHLADYADDLERIVAWAGEPHPLIVGHS
                     LGGLTAITWAKRHPNQASAIVLEDMPLSGGVDRAPMLEGWSQLAAMSVEDVIAYYRQE
                     FPHWSDDDRERRAEVITSTHPAVFTEMVDLAMQGEGIDYLADLGSIRSPILLIHGDFE
                     SGGLVPAPGAARFAVLGPNFSVVRIPGGSHSLHRESTEPFLEALRIFLEGA"
     gene            4740..7010
                     /locus_tag="VHR64_12520"
     CDS             4740..7010
                     /locus_tag="VHR64_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX3303700.1"
                     /translation="MNLSIRRSLSVLAMTLLLTGTLAACGTESQNVEPTVTRVDVAGA
                     PPTRTPTVPGGETPPPSGGAASPAPGGGGEQAGGAPTTVEVDMVDLAFQPTEITIPAN
                     TDVTIKAVNKGALPHTFTIPDVADTGEVPGGSSAEVKVNLPAGDYTFDCSVPGHKEAG
                     MVGTVKVVEGGGAAAAQASPQPAGSPSEGAAAPAGLPTSLEIDMVDLAFQPTELTIPA
                     NTDVTIKAVNKGALPHTFTIPDVEDTGEIASGSSAEVKVNLPAGDYTFDCAVPGHKEA
                     GMVGTLKVVEGGGAAAAPPEQAASAEASPAQTTPAVAGTPEAGGGAALPTTLEIDMVD
                     LAFQPTDLTIPANTDVTIKAVNKGALPHTFTITDVADTGEVASGSSAEVKVNLPPGEY
                     TFDCTVPGHKEAGMIGKLTVVAGSESAGGATTPQTGGTTAEATPAAASPAAGGAAALP
                     TTLEVDMVDLAFEPKDLTIPANTDVTINAVNKGALPHTFTITGVADTGEVAAGASAEV
                     KVNLPAGTYEFDCTVPGHKEAGMVGTLTVVESGGAAPPAASPAASPQASPIATEVNLD
                     MVELRFIPDTLEIPANVDVKVNLSNSGNLPHNFSIKDKDVSVDLAAGDKTSFTLNLPP
                     GTYDFFCNVPGHESAGMVGKLTVVEGGGATPVATPAGQSSEAGATSVEVDMVDLAFQP
                     TEITIAANTDVTIKAVNKGALPHTFTIPDVADTGEVAGGSSAEVKVNLPPGEYTFDCT
                     VPGHKEAGMVGKLIVK"
     gene            complement(7027..7575)
                     /locus_tag="VHR64_12525"
     CDS             complement(7027..7575)
                     /locus_tag="VHR64_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3303701.1"
                     /translation="MSAPANELPFATAQFDVVDCDSIFAYVRNDEGLANELSRITAVG
                     GSVLIRVPAKGPLAVLDAFNLHRYLVDISKRGLRPFETADIGWRRHYSEGDLALMFGP
                     RDFTLTSVRRSGLGASEAMRLSGFVLFRWLRPSRDGYMRLSRAAERVRKAEAQVAWRH
                     GFWLEVELRKHESATRSDSGSR"
     gene            7795..8520
                     /locus_tag="VHR64_12530"
     CDS             7795..8520
                     /locus_tag="VHR64_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303702.1"
                     /translation="MFSLHPVTVTDVSEFLADVAISANSPGAMSIDMVRRGDSRGPDL
                     LGLALATFLANRRPSFVLSESSLTSWEAQIDRGIGMFLRPPARLLIDAGLERPLAQTL
                     AIRLDHSGGSMAGAFVPVHLIGQLGDLLEERLERDLRRLREAELDPVANMGLMLQAVA
                     FARANQTGLIEAMGVTDGLEPGAQVVIADRSRLPKDLRKRLEAAARPPKKPGLVARLF
                     GGRPQSSANGLDQHAASPDDPAF"
     gene            8664..9725
                     /gene="hrcA"
                     /locus_tag="VHR64_12535"
     CDS             8664..9725
                     /gene="hrcA"
                     /locus_tag="VHR64_12535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX3303703.1"
                     /translation="MNQELTQTQPNDPVLTQRQRDILRMVIFDYVQAGRAVASKTLVD
                     RYRMPFSSATIRNEMAVLEEFGLLQVLHTSGGRVPTDSGYRYYVHHLMGNIELPAGDQ
                     IMIRHQFQQAADRLDAWIELAATVLASTSGNVSLVTAPRTVVPRLRHFELIQLQPSVA
                     LVVLVTFESAVRQSMIHLPAETTQDALSRLADGLAPELRGLTADEIETRIPGSDAMAR
                     SVLAQISAMLRQFESAEQVEIRHSGLEHMVGQPDVADLDLQMMMSLVSSGGLMSALLP
                     QISSAGDVQVFIGSDDLPHGLQRFGVVLSPYGIEDVMTGVLGVLGPTRMSYWRTISTV
                     RYMARLMSDLMAELNPRVE"
     gene            9748..10266
                     /locus_tag="VHR64_12540"
     CDS             9748..10266
                     /locus_tag="VHR64_12540"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX3303704.1"
                     /translation="MLVQDTNVVDEQEEGTTSEQPTIESLSAERDQLVDQLQRSVAEF
                     RNYRRRTDEQRAELRAHATRDVMLSILPLIDDLNRAKANIPEDERLDGLREGLEAIDR
                     KFLSVLERNGVSPVGAVGDVFDPAFHEAVATDDSGERSHIVEVYQTGYRQGDASLRPA
                     MVKVGAEPTFQA"
     gene            10317..12236
                     /gene="dnaK"
                     /locus_tag="VHR64_12545"
     CDS             10317..12236
                     /gene="dnaK"
                     /locus_tag="VHR64_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX3303705.1"
                     /translation="MARMIGIDLGTTNSVMATIEGGEPVVIPNAEGERLTPSVVAVSS
                     QGERLVGRFARRQAVTNPENTVFSIKRFMGRKFTDPHVQKDLSLIPYKVREASNGGVE
                     VKMGDRWYSPPEISAMILQKMRADAEAYLGDKVDRAVITVPAYFDNSQREATKDAGKI
                     AGLTVERIINEPTASALAYGLDKKHDEKVAVYDLGGGTYDISILDLSEGVFQVLSTNG
                     DTHLGGDDFDQVIIDFLADEFKKQEGIDLRKDRMALQRLKEAAEKAKIELSSLEQTEI
                     NLPFITADASGPKHLNLTMSRSKLEQLVNPLIERTGPPMQAALKDAGLAKGEVDETVL
                     VGGQTRMPAVQRKVQSYFGKEPHKGINPDEVVAIGAAIQGGVLGGEVKDILLLDVTPL
                     TLSIETLGGVATPLIERNTTIPTRKSQVFTTAADNQPQVEINVLQGERPMAIDNKSLG
                     KFILDGILPAPRGLPKIEVTFDIDANGILNVTAKDQATSKEQKITITGSSGLSDSEVD
                     RMVHEAEEHAEEDRKRREAISLRNDAESMVFQAEKTLTEYGEQIPVEVKTELDEKIQA
                     VKEILDKDRENVDRLKPAYEDMVQTLSKVGTAMYSQAGDAAANGTGFDGASAEQAAED
                     DSTVEGEFREVGSER"
     gene            complement(12292..14523)
                     /locus_tag="VHR64_12550"
     CDS             complement(12292..14523)
                     /locus_tag="VHR64_12550"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX3303706.1"
                     /translation="MAAKTVADDVVSTKINSRTTFDVLANDPAGWSLVDHTDPAHGEL
                     TLHGNEFDYLPNANFVGADAFTYRARRGSAEPLTGTVTIEVTGRVTSVESRDDTLQTD
                     EDVPGTIYVLANDGLGPAGLPLIVTIEHAPEHGSAEVQADNSVRYTPAANWFGTDSFS
                     YFAGDGSTSDSATVSITVNSIDDAPTAVDDAAFIRQDGAASIDVLANDWEADGDEFGF
                     VGNTQPAAGTATWSSVDHMINYEPNPGFFGVDQFTYSIADFDGTGTATVTVTVNGAPT
                     PQDDVASTSQSTPVDVPVLANDADPESDALTVASVGNAAHGKVTINPDSTVRYEPNRH
                     FVGQDSFTYSARDASGNTRDATVTMTVSGSSGSAGGAVDDEFLILEDSPGNTLDVLSN
                     DDSGGGTPSISIDTTPAHGTLTLDAMQSITYVPDPGYIGRDGFTYSISNGGNPRSSAT
                     VSIEIQEVNDAPVAAPDAATGDEDSEIVVDVLANDTDEEGGDLSVDSVASPGNGKARI
                     RNDGNIAYVPNADFSGVDTFDYVVSDGQGGTASSTISVTVNPINDAPQARDDRAHAEI
                     GASIVIDALANDHDPDGPYALLTITRQPEHGTASITYSNTIAYTPMPKYEGYDTIEYQ
                     YSDGLETATASISIAVGNVNSAPVAKDDRVETTTGESVTIDVRANDKDADGDEMIVYG
                     LTQPDHGEVAVENGVVTYTPEPGFVGTDSFTYKVRDGKSGRDKATVQVTVNPTKDRRS
                     SDE"
     gene            complement(14862..>15641)
                     /locus_tag="VHR64_12555"
     CDS             complement(14862..>15641)
                     /locus_tag="VHR64_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303707.1"
                     /translation="GTGGDIRSLVSGDVEYVDGRQSVAIRTVGSRLFGIVGVGPSTEG
                     ALVMAVGGPGEEAQPDRVTAEMRGKIVVAGASVSATTLRRLMDIGAAGVIVGGIVERE
                     VSACFGVPAEDRLSPWRIGPSDTGMGDSMTTAIAIVATEGFGSLPITADAFAFLKKFE
                     GTHVTLITTTRMSGFLARPQIIAVNQDMLDDDAPAQPIALTTETRVRLVDQANLGMTG
                     KVAERPKRVRRADGVAVDVMTITTPDNKDRVVAANNVEVIA"
BASE COUNT         2889 a         4822 c         5136 g         2794 t
ORIGIN      
        1 ccatgaggca ttcgattgcg ccattccagg cgggcacgga gccccgccac tacggacggt
       61 ttgaccggaa tcttgggctg ctccaggcag gtacggagct tcgccatcac cgaaatcaat
      121 tgttggaaac gtttctgatt acggcgagga tgtcctcgcc gtactggttg gccttgatga
      181 cgccgatgcc cggggttctg agcaggtcgg cgcgcgtccg cggacggtcg gccgcgagtc
      241 ggtcgaggag tcggtcggag gcaatgacga aagccggcac gaccatctcg cgcgccttct
      301 cccgacgcca ttgagccaac gcttcgaaat acggatcgtc ccgccgcttg ggaacgacgc
      361 ggcggctctg ttcggcgctc ggtttggtcg tgcaggtatc gcagacatcg ccgcacggat
      421 ccagccgctc gttcaggtga tgcgccaggt ggacatgccg gcatgagcgc gtgctgaggt
      481 agccgatcat ctcctccgcg cgcttcttct ccgatgccgc caatcgctcg agcagtccat
      541 ccatcgcgcc gcggttcgtt ccctcggtcg ggagcaactc caggcaggtc gagaatccct
      601 ggaacgcgca ggagatcccg gtctgctggc tgaccgcccg ctcgaggacg gtcgggttcg
      661 agtccagctc gtcgcaggcg gtagccgtcg gcacggtcac gatcgcgctg ccgttgcgga
      721 tacgcgtgag ccattgcccg aagcgatcgt gcgattgggc ggcatcgact tccgccgagc
      781 ggtgcgacca gcggacggtc atcgtgcggc cagtgtcggg atggcggcgg accatgccgc
      841 cttgctcgag cagaccgatc gcgacaccga catcgacgtc gtccggcgcg aacgacccga
      901 gatcctgccg gtcgatgatg caccaccggc cgacggcgga tcgcctcagg ttgcggtaga
      961 catcgcgcag ctcgtcgatc gtcacggctc gctcgctggc gtggcggcgg attgtgcctg
     1021 tgtcgcccga ggtgtagagc aggatgcagc gggctggctg gccatcgcga ccggcccggc
     1081 ccgactcctg gatgtacgcc tccagtgatc gcgagggcga gaagtggatg atcatccgga
     1141 cgtccggctt gttgatgccc atgccaaagg cgatcgtggc gacgataacc tccgcctggt
     1201 tggcgatgaa ggcgttctgg ttgcgagtgc gatcggctgc cggcatcccg gcgtgatagg
     1261 ggacggcccg gatcttgttg tagcgcagcc ggccggcaag ctgatcggcg tgcatgcggg
     1321 acgggacgta aataatggtc ggcccgctcg cttcccggca gagctcgacg acgcgctcgc
     1381 gtttcgcgtc caggtcccgg tatttctcga ccgaatagaa gaggttttcc cggaaggccg
     1441 aggtgcggac catgctgaac gagcgcgaga gcacgatgcc gatttgatgc gccatctcgg
     1501 ccgttgcggt cgcggtgatc gcgagcgtgg gcggatcgcc gagctcctcg agcgcgcggg
     1561 tcaggaagaa gtaatcgggc cggaagtcgt gaccccacat cgagacgcag tgtgcctcgt
     1621 cgattacgac caggcggatg ccggccgtgc ggatcgcatc gaggaactgc ggttggcgaa
     1681 accgctcggg cgcggcgtag atcaggcggt actcgccgcg acgcacaccg gccagccggc
     1741 gctcttgttc gttcagatcg agcgtgctgt tgatgtaggt ggattgcgcg cgcagcggct
     1801 cgggaagcgt cgtgagctgg tcgtgcatga gcgcgatcag cggcgaaacg acgacgaccg
     1861 ggccatccag cagcagcgcc gggatctgaa atgtgagcga tttgccggcg ccggtcggca
     1921 tgatcgccag tgtgtcgaga ccgtccagac aacgctggat gacctcggcc tgacctgggc
     1981 gaagctcgtc gtatccgaag acgtcgcgaa gggtatcgag gacgcgagga tcggttccct
     2041 ccggctccgg cgcggtcaga actggctccg gctccgcttc cggctcggca aatgcctcat
     2101 tcagctccgc taccagcgtt ttcggaattc cgttcgctgg gggagcgacc aagcgatcca
     2161 cgattgttgc cagctcgcgg tcgccgattc ggtcgatctc agcccgagcc ttggccaggt
     2221 cactttgggc aacccgcgtg ccaaagcggt tctccgcatc gagcaggtgc cgccgggcgc
     2281 cgatgcggtc gccacgggag agggcgatcc tggccagggc aaggggtggg cgggagctcc
     2341 ttgtcgaggc ggtcagaagc gtggacgcga tggcctgcgc ctcgtcgatg cggtcgcgcg
     2401 cgagggcgat ctcgcagcgc atcatctcga tggtcaggtc cgattccggg agccggccca
     2461 gctcgcgatc gatctcggcc aggcgatcga gtcggtcagg ggtatcggat tcgagcagct
     2521 gggtcgcgag cctgatccag gctgtgctgt cgggatagcg cgcgcagcgg agctcgcggg
     2581 tgagcagggc ttcggcggat cgaccttccg attccaggag atccgcgcgg ctgtcgagga
     2641 ttgttccggt ctcgccggtt cgctcggtca aggcgtcgag tacgccgcgg gcgtcggcga
     2701 atcgatgatc gcggcggaga tgcccgaaca gaagccggag cgtcttctct gggatttcgc
     2761 caggtcgggc aagggccagg tcaatcagcg cgcggtcgtc catcacccct cactctgaga
     2821 acgcatgttc gtctttctct catcataccg cagacctcgg atcttccgca gggttgtcgc
     2881 ccggctgccg aaatgacagg atagacggca gcaggagcga cgcggcgcat gcaggccgga
     2941 atgctgacaa ccatagagcg gatgcgctgg gcgacgtacg ggctggtggc cggacttgtc
     3001 gtcggcctct tcctgggctg gatgttccat ggcttcgtgg gcatgattgt ccggctcgtc
     3061 atcattgcga tcatcctcgc gccgttcgtg gtcgcactga tcttctggct caaggtttcg
     3121 aacaagaatc gcatggagcg gaccgagacc ggcatccagg aagcggagtg gcacgacctc
     3181 aacacacccg gctaacgccg gacccgtcaa atctgaccaa tttgcccgga gaatgcgcta
     3241 cactcgcaaa gcccgcgtcc gctgatgcgg gcatctgaat cgccgcacat tggagtacta
     3301 catcaatgcc cgttgatttc actcgcgaag atatccgaga ggggttgaag caagtctatg
     3361 accctgagat cgggatcaat atcgtcgacc tcggcctcgt ctacgacgcc gacatcgccg
     3421 acaacggcga cgtgctggtg acgatgacgt tgacctcgct cggctgcccg ctcggcccgg
     3481 tcatcgtcca ggaagtgcag ggcgcactgg gcgaattcca ggggatcggc gagatcgacg
     3541 tgaagctcgt ctggagcccg ccgtggtcgc ccgacatgat gagcgaggac gccaaagacg
     3601 agctcggcat ctggtagcgg atgccgcccg tgctggccat ccgtagatgc cggcggcgca
     3661 tcggtctggt tttcgcggcc gcaattatcc tgataacgat cggggcgtgc tcaacgggcg
     3721 cgccctcgct cgatcaaccg gaagccaccg tcgcggctcc caccgccacg gccgccggct
     3781 aagcaccttc caggaagatc cgcaacgcct cgagaaacgg ctcagtgctc tcgcggtgga
     3841 gcgagtgcga gccgcccggg atccggacga cactgaaatt ggggccgagc accgcaaatc
     3901 gggccgcgcc cggcgcgggg accagcccgc cactctcgaa atccccatga atcaggagaa
     3961 tcggtgacct gattgatccg agatcggcca ggtagtcgat cccttcacct tgcatcgcca
     4021 gatcgaccat ctccgtgaag actgccggat gggtcgaggt gatcacctcc gcgcgtcgct
     4081 cgcgatcgtc gtcactccag tgcgggaatt cctgccggta ataggcgatc acgtcctcaa
     4141 ccgacatcgc cgcgagttgt gaccagcctt cgagcatcgg agcgcgatcg accccgccgc
     4201 tcaacggcat gtcctcgagc acgatcgcgc tcgcttgatt cgggtggcgc tttgcccagg
     4261 taatcgcggt caggccgccg agcgagtgac ccacgatcaa aggatgaggc tcgccagccc
     4321 aggcgacgat ccgctccagg tcatcggcat agtcggcgag gtggtagccg tggtccggtt
     4381 gaccggactg cccgtggccg cgcaagtcgg ggatgatcag ccggtacaac tcggccagcc
     4441 ggctcatgac cggcatccag ctcatgccac tgctgccgat gccgtggagc aggatcatcg
     4501 ttggaccaga cccgatcgtc gccacggcga tgtccggacc gctgccggca atgacggatc
     4561 gcttcaacgt tcgggtctgt tcggtcacgc tgaactccgg gcgcaagggt gaaaagacgc
     4621 gcatttgtgc gccaatcacg gcagggttta ccgctggaga gtaccattgc cgatgcgtat
     4681 ggaagatgcc agcgtcgctt tgtgctggca ttcggacggg aaggcggagg agtgcctcgg
     4741 tgaacctaag cattcggcgg tcgctgtcgg tattggcaat gacgttgctt ctgacaggga
     4801 cactggcggc gtgcggaacg gaatcgcaga atgtcgagcc gaccgtaacc cgtgtggatg
     4861 tggccggtgc accaccgacc cgcactccga cggttcccgg cggtgagact cctcctccga
     4921 gtggcggcgc agcgtcgccg gctcctggcg gaggtggtga gcaggcaggc ggcgcgccga
     4981 cgacggtcga agtggacatg gtcgacctcg ccttccagcc gaccgagatc acgatcccgg
     5041 ccaacaccga cgtgaccatc aaggccgtca acaagggcgc cttgccgcac accttcacga
     5101 tcccggatgt tgctgatacg ggcgaagtcc ccggtggctc aagcgccgag gtcaaggtca
     5161 acttgccggc gggtgactac accttcgatt gctccgtgcc ggggcacaag gaagccggca
     5221 tggtcggcac agtgaaggtg gttgagggcg gtggagcggc cgcggctcag gcctctccgc
     5281 agccggccgg ctcgccgagc gaaggcgcag ctgctcccgc aggtctgccg acgtcactcg
     5341 aaattgacat ggtcgatctg gccttccagc cgacagagct cacgatcccg gcgaacaccg
     5401 atgtcacgat caaggccgtg aacaagggtg cgctcccgca tacgttcacg attccggacg
     5461 tcgaggacac gggcgagatc gccagcggtt cgagtgccga agtcaaggtc aacctcccgg
     5521 ccggcgatta cacgtttgac tgcgcggtgc ccggccataa agaggccggg atggtcggta
     5581 cattgaaggt cgtcgaaggc ggtggagcgg ccgcggcgcc acccgaacag gccgcgtcgg
     5641 cggaagcaag cccggcccag acaactccgg cagttgcggg cacgcctgaa gccggaggcg
     5701 gagcggcgct tccgaccacg ctcgaaatcg atatggtcga tctcgcgttt cagccaacgg
     5761 atctgacgat cccggcgaac accgacgtca cgatcaaggc ggtcaacaag ggagcgcttc
     5821 cgcacacctt cacgatcacg gacgtggctg atacgggcga agtcgccagc ggctcgagcg
     5881 ctgaagtgaa ggtcaatctc ccaccgggtg aatacacatt cgactgcaca gtgcctggtc
     5941 acaaggaagc gggcatgatc ggcaagctga ccgtcgtcgc cggcagtgag tcggcgggcg
     6001 gcgcgaccac cccgcagacg ggtgggacga cggccgaggc aacgcccgcc gccgctagcc
     6061 cagccgctgg tggcgcggca gcgctcccga cgacgcttga agtcgatatg gtggatctcg
     6121 cgttcgaacc gaaagatctg accatcccgg cgaacacgga cgtgaccatc aacgcggtca
     6181 acaagggtgc gttgccacat acctttacga tcaccggagt agcggatacc ggcgaagtcg
     6241 ctgctggtgc gagcgccgag gtgaaggtca atctcccggc cggaacctat gagttcgact
     6301 gcactgtgcc cggacacaag gaagccggaa tggtcggtac attgactgtg gtcgaaagcg
     6361 gtggcgcggc gccgcctgcg gcgagcccgg cggccagtcc gcaggcgagc ccgatcgcga
     6421 ctgaagtcaa cctggacatg gtcgagctgc ggtttatccc ggacacgctc gagatcccgg
     6481 ccaacgtcga cgtcaaggtc aacctgtcga atagcggcaa tctaccgcac aacttctcga
     6541 tcaaggacaa ggacgtctcg gttgatctgg ccgcaggcga caagacgagc ttcacgctga
     6601 acctgccgcc cggcacctac gacttcttct gcaacgtgcc gggtcacgag agcgcgggca
     6661 tggtcggcaa actgaccgtg gtcgagggcg gcggcgcgac tccggttgcc acgccggcgg
     6721 gacaatccag cgaggcgggt gcgacgtccg tagaggtcga catggttgat ctggccttcc
     6781 agccaaccga gattacgatt gccgcgaata ccgatgtgac catcaaggcg gtcaacaagg
     6841 gagcgctgcc ccataccttc acgatcccgg acgtcgccga taccggtgag gtggccggtg
     6901 ggtcgagcgc tgaagtcaag gtcaatctgc cacctggcga gtacaccttc gactgcaccg
     6961 tgcctggtca caaagaggcc gggatggtcg gaaagttgat cgtcaagtag cgcgaagtaa
     7021 cgtcagtcag cgcgatccgg agtcactccg ggtcgcgctt tcgtgtttgc ggagctcgac
     7081 ttcgagccag aagccgtgtc gccatgcaac ctgcgcctct gcctttcgca cacgctcggc
     7141 ggcccggctc aggcgcatat acccgtcacg cgatggccgc aaccaccgga agagcacgaa
     7201 cccgctcaat cgcatcgcct cggacgctcc aagtccagac cgccgcacac tcgtcagcgt
     7261 gaaatcgcgc ggtccgaaca tgagcgcgag gtcgccttcg ctgtagtgcc tcctccagcc
     7321 gatatcggca gtctcaaatg ggcgcaggcc gcgcttcgag atgtcgacca ggtagcggtg
     7381 caagttgaag gcatcgagca ccgccagcgg tcccttggcc ggcactctga tcaagaccga
     7441 gccgcccacc gcggtgatcc gtgacagctc gttggccaga ccctcgtcat tgcggacgta
     7501 cgcaaatata gagtcgcagt caacaacatc gaattgggcg gtcgcgaacg gcaattcatt
     7561 cgccggagct gacatcgatg agccggccgt ttcgcagcgg agcggccggt cctcttcgcg
     7621 cggctcgccc cgcggtcgac cggagcgtgg ttttgttcgg tgccaacgcc aatcaagacc
     7681 gatccgcagg caacgggacg agtctgaagt gaaggaggag ttcgtcatct cgcctctata
     7741 atcaggtctg cgtgccggat ctgccaattg tgcggtgcgc cgggagatgc ccacgtgttc
     7801 tctctccatc ccgtcaccgt caccgatgtc tccgagtttt tggcggatgt tgcaatcagc
     7861 gccaactcgc ccggtgccat gtctatcgac atggttcgcc ggggtgattc gcgcggaccg
     7921 gatctgcttg gtctggcact ggcgaccttc ctcgcgaacc gccgtccaag cttcgtgctg
     7981 agcgagagta gtttgacgag ctgggaggcg caaatcgatc ggggaatcgg gatgtttttg
     8041 cgccctcctg ccaggctcct catcgacgcc gggctggagc ggccgcttgc gcagacattg
     8101 gcgatccgcc tcgaccacag tggcgggagt atggccggcg cgttcgttcc ggtgcatctg
     8161 attggccaac tgggtgacct gctggaggag cggctcgagc gcgatctgcg caggttacgc
     8221 gaggcggagc tggatccggt cgccaacatg gggctcatgc tccaggcagt ggcattcgcc
     8281 cgcgcgaatc agacgggcct gatcgaggcg atgggtgtga cggacgggct ggagccggga
     8341 gcgcaggtcg ttatcgcaga ccgcagccgc ttgccaaagg atcttcgcaa gcgactggaa
     8401 gcggctgcca ggccgccaaa gaagcccggt cttgttgccc gactgttcgg cgggcgacca
     8461 caatcgagcg ccaatggact tgatcagcat gccgcctccc cggacgatcc agccttctga
     8521 aaccaactca ggaccgtctt atctgctccc agatcggaca ttcagggttg tttaaccttg
     8581 acacggtccg tgtaaagttg ctatcctatt tggcgttagc actctttgcg agagagtgct
     8641 aaccagccta gtgattgcgt gagatgaatc aggaactgac ccaaacgcaa ccaaatgacc
     8701 cggtgctaac gcagcgacag cgcgacattt tgcgcatggt gatctttgac tacgttcagg
     8761 cgggtcgggc cgtcgcgtcg aagaccctgg tcgaccgata ccgcatgccg ttctcgtcgg
     8821 ccacaatccg gaatgaaatg gcggtcctcg aggagttcgg gctgcttcag gtcctccata
     8881 cctcgggcgg gcgagtacca accgactccg ggtatcgcta ttacgtccat cacctgatgg
     8941 gcaacatcga gctcccggct ggcgaccaga tcatgatccg ccatcagttc cagcaggcag
     9001 ccgatcggtt ggatgcctgg atcgagttgg cggcgactgt gcttgcttcg acgagtggca
     9061 acgtatcact cgtcacggcg ccgcggaccg ttgtgcccag gctgcgccat ttcgagctga
     9121 ttcagcttca gccgtccgta gccttggtcg tcctggtcac ctttgagagc gccgtccggc
     9181 agtccatgat tcatttgccg gccgagacta cgcaagacgc attgtctcgt ctggccgatg
     9241 ggctcgcgcc ggagttgcgg ggcctgaccg ctgacgaaat cgagacccgg atccccggtt
     9301 ccgatgcgat ggcgcggtcg gttctggccc agatctcggc gatgcttcgc cagttcgaat
     9361 cggccgagca ggtcgagatt cgacactctg gcctcgagca catggtgggt cagccggatg
     9421 tggccgatct ggacttgcaa atgatgatga gcctggtcag ctcgggtggg ttgatgtctg
     9481 ccctgttgcc gcagatttcg tccgccggcg atgtccaggt ctttatcgga agcgacgatt
     9541 tgccgcacgg gctgcagcgg tttggtgtcg tgctctcgcc ctacgggatc gaggatgtga
     9601 tgaccggcgt gctcggcgtg ctcggtccga cccggatgtc gtactggcgc acgatctcga
     9661 ccgttagata catggcgcgg ttaatgagcg acctgatggc agagctcaac ccgcgcgttg
     9721 aatgagggcg tactgatgaa ggaggacatg ttggtacaag acacgaatgt cgtggacgag
     9781 caggaggagg gaacgacctc cgaacagccc acgatcgaat cgctgtccgc tgagcgcgac
     9841 cagttggtcg atcagctcca gcgcagcgtt gccgagtttc gaaactaccg gaggcggacg
     9901 gacgagcagc gagcggagct gcgggcgcat gcgacgcgcg acgtgatgct ctcgatcttg
     9961 cccctaatcg acgacctcaa ccgcgccaaa gcaaacatcc cggaggatga gcggctggat
    10021 ggtctccgag aagggctcga agcgatcgat cgcaagttcc tgagcgttct cgagcgcaac
    10081 ggcgtcagcc cggtcggcgc ggtgggcgat gtcttcgatc ccgccttcca cgaagcggtc
    10141 gcgaccgacg acagcggcga gcgctcgcac atcgtggagg tctaccagac gggctatcgt
    10201 cagggagatg cgtcactgcg cccggcgatg gtcaaggtgg gcgccgaacc cacatttcag
    10261 gcatagcgaa cacaggcgat tgagacaggt ttagcagaga gaaggaaatc agagcgatgg
    10321 cacgaatgat tggtatcgac ctcggaacga ccaactcggt gatggccacc attgagggtg
    10381 gcgagccggt tgtcattccg aacgcggagg gtgagcgtct caccccgtca gtggtggcgg
    10441 tcagctcgca gggcgagcgg ctggtcggcc ggtttgcccg gcgccaggcg gtcaccaacc
    10501 ccgagaacac ggtcttctcg atcaagcggt tcatgggtcg caagttcacc gacccacatg
    10561 tccagaagga cctctcgctg atcccgtaca aggttcgcga ggcgtcgaat ggcggcgtcg
    10621 aggtcaagat gggcgaccgc tggtacagcc cgccggaaat ctcggccatg atcctgcaga
    10681 agatgcgagc cgacgcggag gcgtacctcg gcgacaaggt cgatcgggcg gtgatcaccg
    10741 tcccggccta ctttgacaac agccagcgcg aggccaccaa agacgccggc aagatcgccg
    10801 gactgacggt cgagcggatc atcaacgagc cgaccgcatc ggccctggcc tatggcctcg
    10861 acaagaagca cgacgagaag gtcgccgtct acgacctcgg tggcggcaca tacgacatct
    10921 cgatcctcga tctctccgag ggcgtcttcc aggtgctgtc caccaacggc gacacgcacc
    10981 tgggtggtga tgacttcgac caggtgatca tcgatttcct ggcggacgag ttcaagaagc
    11041 aggaaggcat cgacctgcgc aaggatcgga tggccctgca gcggctcaag gaagccgccg
    11101 agaaggccaa gattgagctc tcctcgctgg agcagaccga gatcaacctg ccgtttatca
    11161 cggcggatgc gagtggcccg aagcacctga atctgaccat gagccgatcc aaactcgagc
    11221 agctggtcaa tcctctgatc gagcgcactg gcccgccgat gcaggccgcg ctcaaggatg
    11281 ccggtctggc gaagggcgag gtcgatgaga cggtcctggt cggtggccag acccggatgc
    11341 cggccgtgca gcggaaggtc cagagctact tcggcaagga gccgcacaag ggcatcaacc
    11401 cggatgaggt tgtcgcgatc ggcgcggcga tccagggcgg cgtgctcggt ggcgaggtga
    11461 aggacatcct gctcctcgac gtcactccgt tgaccctttc gatcgagacg ctgggcggcg
    11521 tggccactcc gctgatcgag cgcaacacga ccatcccgac ccgcaagagc caggtcttca
    11581 ccacggcggc ggacaaccag ccgcaggtcg agatcaacgt cctgcagggc gaacggccga
    11641 tggcaatcga taacaagtcg ctcggcaagt tcatcctcga cggcatcctg ccggcgccgc
    11701 gcggccttcc gaagatcgaa gtgaccttcg acatcgacgc gaacggcatc ctgaacgtga
    11761 ccgcgaagga tcaggcgacc agcaaagagc agaagatcac gataaccggc tcgtccggtc
    11821 tgtcggattc cgaggtcgac cggatggtgc acgaggccga ggagcatgcc gaggaggacc
    11881 gcaagcggcg cgaggcgatc tcgctgcgga acgatgccga gagcatggtc ttccaggccg
    11941 agaagacgct gaccgagtac ggcgagcaga tcccggtcga ggtcaagact gagctggacg
    12001 aaaagatcca ggcggtcaag gagatcctcg acaaggaccg cgagaacgtc gatcgactca
    12061 agccggccta cgaggacatg gtccagaccc tgtccaaggt cggcacggcg atgtacagcc
    12121 aggccggtga tgcggccgcg aacggcaccg gattcgacgg cgcgagtgcg gagcaggcgg
    12181 ctgaggacga ctcgacggtc gagggcgagt tccgcgaggt cggctcggag cgctaactcc
    12241 ggaccgtcac atcacgagtc tgaagatgag gggtgcgggc aaccgcgccc ctcattcgtc
    12301 gctggagcgg cgatctttgg ttgggttgac agtgacctgg acggtggcct tgtcgcggcc
    12361 gcttttgcca tcgcgcacct tgtaggtgaa ggagtcggta ccgacgaagc ctggctcggg
    12421 cgtatacgtg acgacgccgt tctcgacagc cacctcgcca tggtccggct gggtcaggcc
    12481 gtagacgatc atctcgtcgc catcggcatc cttgtcgttg gctcgcacat cgatggtgac
    12541 ggactcaccg gtggtggttt ccacccggtc gtccttcgca acgggcgccg agttcacatt
    12601 tccgaccgcg atcgaaatcg atgccgttgc cgtctcaaga ccatccgaat actggtattc
    12661 aatcgtgtca tagccttcgt acttcggcat cggggtgtaa gcgatcgtgt tgctgtacgt
    12721 gatcgaagca gtgccgtgct ccggttgccg ggtgatggtc agcagggcat acggaccatc
    12781 cggatcgtga tcgttggcaa gcgcatcgat gacgatgctc gcgccgatct cggcgtgcgc
    12841 tcgatcgtcc ctggcttgcg gtgcgtcgtt gatcgggttg acggttaccg aaatcgtgct
    12901 gctggccgtc ccgccctgac catcggacac gacataatca aaagtatcga ccccggagaa
    12961 gtccgcattt ggcacgtacg caatgttgcc gtcattccgg atccgggcct tgccattccc
    13021 cggcgaggcc actgagtcga cggacagatc gccgccctcc tcgtcggtgt cgtttgcgag
    13081 gacatcgacc acgatctccg agtcctcgtc gccggtagcc gcatccggtg cggcaaccgg
    13141 ggcatcgttc acttcctgga tttcgatcga gacggttgcg ctggagcgcg ggttgccgcc
    13201 gttactgatc gagtaggtga agccgtcccg gccgatgtac ccggggtccg ggacgtaggt
    13261 gatgctctgc atcgcatcga gcgttagcgt cccatgggcc ggagttgtgt cgatcgagat
    13321 cgagggcgtg ccgccgcccg agtcgtcgtt cgaaaggacg tcaagcgtgt tccccggaga
    13381 atcctcgagg atcaggaact catcatcgac cgctccgcct gccgaacccg acgacccgga
    13441 gacggtcatg gtgacagtcg catcccgcgt gttccctgat gcatcgcgcg cggaataggt
    13501 aaacgagtcc tggccgacga aatggcggtt gggctcgtag cgcacggtgg aatccgggtt
    13561 gatggtgact ttgccgtgcg cggcgttgcc gacgctcgcg acagtcagcg cgtcgctttc
    13621 cggatcggcg tcgttagcga ggacgggcac gtcgaccggg gtcgactggc tggttgaggc
    13681 gacgtcatcc tggggtgtcg gcgcgccgtt gaccgttacg gtgacggtcg cggtgcccgt
    13741 tccgtcgaaa tcagcgatgg agtaggtgaa ctggtcgaca ccaaagaagc cgggattcgg
    13801 ttcgtagttg atcatgtggt cgacgctgga ccaggtcgcc gttccggccg caggttgcgt
    13861 gtttccgacg aagccgaact cgtcgccgtc agcttcccag tcgttcgcga gcacgtcgat
    13921 cgacgcggcg ccgtcttgtc tgatgaaggc ggcgtcgtcg acggcggttg gcgcatcgtc
    13981 gatcgaattg acggtgatcg agaccgtcgc actatcggac gtcgagccat cgccggcgaa
    14041 gtacgaaaac gagtcggtgc cgaaccaatt cgccgccggg gtgtaacgga ccgagttgtc
    14101 cgcctgcacc tcggcgctcc cgtgctccgg cgcgtgctca atagtcacga taagcggcag
    14161 tccagccggc cccagtccgt cattcgctag cacatagatt gttccgggga cgtcctcgtc
    14221 agtctggagc gtgtcgtccc ggctttcgac ggaggtaact cgaccggtga cttcgatcgt
    14281 gaccgtgccg gtcagcggct cggctgaacc gcggcgagcc cgatacgtga atgcgtcggc
    14341 gccgacgaag ttggcattcg gcaggtaatc gaattcgttg ccatgcaacg tcagctcgcc
    14401 atgcgccgga tcggtatggt cgacgaggga ccagccggcc gggtcgttgg cgaggacatc
    14461 gaacgtcgtc cgggaattga tcttcgtgga gaccacatcg tccgcaacag tcttcgccgc
    14521 cacagcgcct ggagcagacg agaccgtagg accgaggagg gcgccggtaa ttgccagaac
    14581 cagactggct ctgcgaatgc ccgcagcgaa tttcgaccgc gacggtgaag caacgctctt
    14641 cgcgatcggc gcgaggattg tccacgggtg aaccggcata gttcgagttt ctttcaggta
    14701 gttcgtacaa gtacttctac accgtaccga tcctggatac aacgggaaat cacccctcgg
    14761 tatggttttt cgataccagg gtgcctattt cagcccaaca cagaagctgg cgacgcccgc
    14821 acgagcgtcg ccagctttct cattcgatgt tgaagagcgc gctatgcgat gacttccaca
    14881 ttattggcgg cgaccacccg gtccttgttg tccggtgtcg tgatcgtcat cacatccacg
    14941 gccacgccgt ccgcgcgccg aacacgcttc ggccgttcgg cgactttccc ggtcattccg
    15001 agattcgcct gatcgacgag acgaacccgc gtctcggtcg taagggcgat cggctgagcc
    15061 ggcgcgtcgt cgtccagcat gtcctggttg acggcgatga tctgcggccg ggccaggaat
    15121 ccgctcatcc gggtggtcgt aatcagcgtg acgtgcgtgc cctcgaactt cttcaggaag
    15181 gcgaacgcat ccgccgtgat cggcagggaa ccgaagcctt ccgtagcgac aatcgcgatc
    15241 gcggtggtca tgctgtcgcc catgccggtg tcggatggcc cgattcgcca cggggagagc
    15301 cgatcctcgg ccgggacccc gaagcatgcg gaaacctcgc gctcgacaat tccgccgacg
    15361 atcacgccgg ccgcgccgat atccatcagc cgccgcaggg tcgtcgcgct gacactggcg
    15421 ccggctacga cgatcttgcc gcgcatctcc gctgtgaccc gatcaggctg tgcctcctcg
    15481 cccgggccac ccaccgccat gacgagagcc ccctccgtcg acggaccgac tccgacgatc
    15541 ccgaacaggc gggagccgac ggtgcggatt gcaaccgact ggcgaccgtc gacgtactcg
    15601 acgtcgccgg agaccagcga gcggatgtcg ccaccggtgc c
//
LOCUS       DATAOZ010000486         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3614869, whole genome shotgun sequence.
ACCESSION   DATAOZ010000486 DATAOZ010000000
VERSION     DATAOZ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3614869"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..500)
                     /locus_tag="VHR64_12560"
     CDS             complement(9..500)
                     /locus_tag="VHR64_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303708.1"
                     /translation="MNVRMRFAAFCVAAVALLGAAAVIGGSAFAQDSTGTPEAGSGTP
                     AVGSDTQASAYQDFVDGLAANLGISDPATVDAAIKTTLKQQVDAELAAGNISANDATA
                     LKERIDAADFGPFLFGVGQHDGHGIGGPGRGGHDHPGTGKNDNEQDDGSGASPTGTPT
                     SGL"
     gene            complement(1132..1629)
                     /locus_tag="VHR64_12565"
     CDS             complement(1132..1629)
                     /locus_tag="VHR64_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303709.1"
                     /translation="MALIALGVVVHQGGFAQDDYSTPPATTGGQDGYTTPAAGTNSAA
                     TDAKAEASLQYLSFIKAIAANLGVSDPMTVDSGIRTMLKQVVDDRHNAGEISDSAASS
                     LDAQIDAGNVAPLIFGQPGQTGASASGNDPSDTGAQQGDDNNTQQQQQAPTETPDSGD
                     YDYGY"
     gene            complement(1820..2212)
                     /locus_tag="VHR64_12570"
     CDS             complement(1820..2212)
                     /locus_tag="VHR64_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303710.1"
                     /translation="MQAETITTVINRQRDDVFAYLNDINNVTEWAFNFAKELKVINGS
                     HKVVTPAGELFLRYDSHPDLGLIDMWGGPAEDQMTPLPVRVLPLANGSSAVTITLTQT
                     PDVDDAVFKKQVASLNEESAGLKTRLEK"
     gene            complement(2495..>3390)
                     /locus_tag="VHR64_12575"
     CDS             complement(2495..>3390)
                     /locus_tag="VHR64_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX3303711.1"
                     /translation="FAITTMLQRKGTVRAVDLAAAFEVSTRTIYRDITALSEAGVPVV
                     SLPGKGYTLTEGFFLPPLVFTTGEAAAVALGTRMLATQASGRHVKEAERALEKIAAVL
                     PNDVRDEVDRLNGIITISHPPGRFDLDDPNLVKLQQAILEHRVVSITYHSRTGDEITE
                     RQVEPVELAYIDHIWYLGGYCRLRRAHRSFRVNRMTSLTIGEDRFISKPSSRGERRPI
                     EVKVRFAEEVLPWVRERPHWSFEREEADGDGAVMTFLPDHLTEISSWILSWGAAAEPL
                     TPPELRALIRAEAGAIAERLT"
BASE COUNT          598 a         1051 c         1041 g          700 t
ORIGIN      
        1 tcgacgttct acaatccgct cgtcggggtt ccggttgggc tcgcgccgga accgtcatct
       61 tgctcgttgt cgttcttacc tgtgcctggg tgatcgtggc cgccgcggcc ggggccgccg
      121 atgccgtggc catcatgttg accgacacca aagaggaagg ggccgaagtc ggcggcatcg
      181 atacgctctt tgagcgcggt cgcgtcgttg gccgagatat tgccggccgc cagctcggca
      241 tccacctgtt gcttgagcgt ggtcttgatc gcggcgtcaa ccgtcgccgg gtcgcttatg
      301 ccgaggttcg ccgccaagcc gtcgacgaaa tcctgatacg ccgacgcctg cgtatccgac
      361 ccgacggccg gtgtgccgct gcccgcttca ggcgttccgg ttgaatcctg ggcgaaggcg
      421 cttcctccga tcacggccgc tgcgccaagc aacgcaaccg cggccacaca gaaggccgcg
      481 aaccgcatcc gaacattcat gattctctcc taacccttcc gggccgccgc atggtcgcgc
      541 cggcccggag ctaacgaccg cgcccgcttt caagcccgtt tgggacatgt tgcgcgtgac
      601 gccacgctag cgttcgttcc ttgagagaac ctggaaaaac gctcaaattc ggctggtaat
      661 gcaggcgaca ggcgtcaggc gtcaggcatc aggcgtcagg catcaggcgt taggcatcag
      721 gcgttaggct gctccccgtc atgctgagcc ctcaccccgt catgctgagc cgccccccgt
      781 catgctgagc gcagtcgaag catctcgcct ctccgggacc gtatcgttgg acaggacaga
      841 tttctcgact ccgcgcgaaa tgacggggga tgaggcgcgc gaaatgacgg ggagggcggc
      901 atcgaaataa cggggtgcac tcggactaac ggcaggaggg gtcaatcctg ccgaagtctc
      961 cccgagtcta agaacgaata attcctggcg ggttgtcgct ctcagtcccg gagggacttc
     1021 gtctggatta gcccggggtt aaaaccccgg gaatcacgat tagcgctcgg tttcaacccg
     1081 gcgtcgcggc gcagccgcgt cgaaagggca gcgccgtcct agtacatcgc cctagtatcc
     1141 gtagtcgtag tcgccggaat ccggcgtttc ggtcggcgct tgctgttgct gctgtgtgtt
     1201 gttgtcgtcg ccctgttgcg caccggtgtc actgggatca ttcccgctgg cgctcgcgcc
     1261 agtttgcccc ggttggccaa agatcaacgg ggccacgttg cccgcgtcga tttgcgcatc
     1321 gagggatgag gcggcgctat ccgaaatctc accggcatta tggcggtcgt cgacgacttg
     1381 tttcaacatg gtcctgatcc cggagtcgac agtcattgga tcgctcacgc cgagattggc
     1441 cgcgatcgcc ttgatgaagc tcaaatactg cagggaggct tctgccttcg catcggtcgc
     1501 cgccgagttt gtaccggctg cgggcgttgt gtagccgtct tgcccgcctg ttgtagccgg
     1561 cggcgtcgaa tagtcatcct gggcgaagcc gccttggtgg acgacaacac cgagagcgat
     1621 cagcgccaac gcggcgacgc tgaacgcgaa aagtcttatt cgatggcgca tggttctctc
     1681 ctgaccgacc ttgctgggtc gccgcggccc agcatgaacg cgtttccctc cccaggagcg
     1741 ccgcgcacgt aagaccaggt tagttgctga accttgagag aacctgaagg aatgcgttgg
     1801 tttggctgag ttgcccctgt tacttctcta atcgcgtctt caagcccgcc gactcttcgt
     1861 tcaacgacgc cacttgcttc ttgaagacgg catcgtcgac gtcgggtgtt tgggtgaggg
     1921 tgatggtgac ggcggacgag ccgttcgcaa gaggaagaac ccgcacggga agcggggtca
     1981 tctgatcttc ggcaggaccg ccccacatgt cgatcaggcc gaggtccggg tgcgaatcgt
     2041 aacggaggaa gagctccccg gcgggcgtga caaccttgtg actcccgttg atgaccttca
     2101 attctttcgc gaaattgaat gcccattcgg tgacattgtt gatgtcattg agatacgcga
     2161 acacgtcgtc tcgttgccgg ttgatgacgg tagtgatcgt ttccgcttgc atgaaaaacc
     2221 tcctgttctg ttcttactat cgatgcctgc aaggatggtt ccacgagtct attgggggtg
     2281 tgacccagag cgccgggagc tgttctcctg ggaattcgtc tctctttcat ctctggaagg
     2341 cgcccattct gtcgtagccg catgccctct gggcgatttt caactctggg accactcgtc
     2401 gctcaaacag gcgccccccg aattcgtagc cgtctgacgc ctgatgcctg atgcccgacg
     2461 cctactgcca tcttgcctca cgagtgtgac aacgctatgt cagtcgctcg gcaatggccc
     2521 cggcttccgc tcgtatcaac gcgcgtagct caggcggtgt gagaggctcg gccgctgccc
     2581 cccaactgag aatccaggaa gagatttcag tcaggtgatc tggcaaaaag gtcatcaccg
     2641 ccccatcgcc gtccgcttcc tcgcgctcga acgaccagtg cggacgctcc cgcacccagg
     2701 gcagcacctc ttcagcaaag cgcaccttga cctcgatcgg ccggcgctcg ccgcgtgaag
     2761 atggcttgga gatgaatcga tcctccccga tcgtcagcga ggtcatgcgg ttgacgcgaa
     2821 acgagcgatg ggcgcggcga aggcggcaat aaccaccgag gtaccagatg tgatcgatgt
     2881 aggcgagctc gaccggctcg acctgacgct ccgtgatctc gtctccggtc cggctgtggt
     2941 aggtgatcga aaccacgcgg tgttcaagaa tcgcctgctg gagcttgacg agattgggat
     3001 cgtcgagatc gaaccgtccg ggtgggtggg agatagtgat gatcccgttc agacgatcaa
     3061 cttcgtcgcg cacatcattt ggcaagaccg ccgcaatctt ctccagcgcc cgttccgcct
     3121 ccttgacgtg gcgtccgctc gcctgggtcg ccagcatccg cgtgccgagc gcgaccgcgg
     3181 ccgcctcgcc cgttgtgaag acgagtggtg gcagaaagaa tccttcggtg agggtatacc
     3241 ccttgccggg gagcgacacc accggtacgc cggcctcgct cagcgccgta atgtcccggt
     3301 agatcgtccg cgtgctgacc tcgaacgcgg ccgcgagatc gaccgcgcgc accgtcccct
     3361 tccgctgcag catggtggtg atggcgaaga
//
LOCUS       DATAOZ010000487         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2303565, whole genome shotgun sequence.
ACCESSION   DATAOZ010000487 DATAOZ010000000
VERSION     DATAOZ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303565"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VHR64_12580"
     CDS             complement(<1..1034)
                     /locus_tag="VHR64_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3303712.1"
                     /translation="MPDRSRAANALTIMMTDVVGSTELRRARGDQDADGILALQAAIV
                     QLQVAAFGGRVRKSMGDGFLISFPSDVAAVRAAVAIQHALDEHNTADPQRAVEVRIGV
                     HSGQVAERDGDLLGQAVHAAARVMTEAVGGEILITDEVRKQAEPQLDYSFLDSGLFWL
                     RGLPELWRLYEVSWNDTSAGPQPGDVPTPLTAFVGRDAERARLRRLVDEALVGRGRLA
                     LVAGEAGVGKSRLVAEIGDEAQARGMQVLTGHCVEMSGTPPYRPYVEIIEQTISSPRS
                     SVALREALGDVAPEIARIAPALRRAFPEIPPAVELPAELARRYVWNSFSEFVGRAAQR
                     QPLLLVLEDL"
     gene            complement(1148..1558)
                     /locus_tag="VHR64_12585"
     CDS             complement(1148..1558)
                     /locus_tag="VHR64_12585"
                     /inference="COORDINATES: protein motif:HMM:NF020413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HEX3303713.1"
                     /translation="MDLERFVRAQNSGGTYQRALTELRNGRKTSHWMWFIFPQIAGLG
                     RSAMAQAYAISDLAEAHGYLAHPVLAPRLVECAEIVASHSGLTAEHIFGGIDAIKLRS
                     SMTLFAHADPDQTIFQQVIDQYYDGPDPETERRL"
     gene            complement(1701..>1936)
                     /locus_tag="VHR64_12590"
     CDS             complement(1701..>1936)
                     /locus_tag="VHR64_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX3303714.1"
                     /translation="AGEVSADGSTLAEVLDSLETNHPGIRGRIIDDAGALRRFVNVYV
                     GDDDVRFIGGLDAKVAEGTKISIIPAVAGGAER"
BASE COUNT          350 a          654 c          610 g          322 t
ORIGIN      
        1 tgcaggtctt ccagtacaag cagcagcggc tggcgctgcg cggcacggcc cacgaactcg
       61 ctgaagctgt tccacacata acggcgtgcc aactccgcag gcaactcgac agctggtggg
      121 atctccggga aggcccgccg cagcgcaggg gcgatacggg ctatctccgg ggccacgtcg
      181 ccgagcgctt cccgaagcgc gacagagctg cgtgggctgc tgatcgtctg ctcgatgatc
      241 tccacgtaag gccggtacgg cggggtgccg ctcatctcca cgcagtgccc ggtaagcacc
      301 tgcatgccgc gcgcctgggc ctcgtcgccg atttcggcaa ccagccgtga cttgccgacg
      361 ccagcctcgc ccgcgacgag cgctaagcgg ccgcgaccaa cgagcgcctc gtcgaccagc
      421 cggcgcagcc gggctctctc ggcgtcccgc cccacaaagg cggtaagggg cgtcggcaca
      481 tcaccgggct gtggtcccgc tgacgtgtcg ttccaggaca cctcgtacag cctccatagc
      541 tccgggagtc cccgcagcca gaaaagcccg gagtcgagga acgagtagtc cagctgcggc
      601 tcggcctgct tgcgtacctc gtcggtgatc aggatctcgc cgccgactgc ctcagtcatg
      661 acgcgggccg ccgcatgcac ggcctgcccg agcagatcgc catcccgctc cgcgacttgg
      721 ccggaatgga cgccgatgcg gacttcgaca gcgcgttggg ggtcagcggt gttgtgctcg
      781 tccaacgcat gctggatcgc cacggcggca cggaccgcgg ccacatcgga ggggaaggag
      841 atgagaaacc cgtcgcccat ggatttacgc acccgaccgc cgaatgctgc gacctggagc
      901 tgcacgatcg ctgcctgcaa cgcgagaatg ccgtcggcgt cctggtcgcc acgtgcccgc
      961 cgcagctcgg tagagccgac gacgtcggtc atcatgatcg tgagcgcgtt cgctgcgcgg
     1021 ctccgatccg gcaaggactg cctccctgtt gctactcagc tgcggatctg ccgttcaaac
     1081 tacggcgccg aggtgtagct acattgcaca cggtgacggt cctggcatca agttgatcaa
     1141 ggaatcgtta caggcgccgc tcggtctccg gatcggggcc gtcgtagtac tgatcgatga
     1201 cctgctggaa gatcgtctga tcaggatcgg catgcgcgaa gagggtcatc gaggagcgca
     1261 gcttgatcgc gtcaatgccg ccgaagatgt gctctgcggt gagtccgctg tgcgaggcga
     1321 cgatctcggc gcactcgacc agccgtggcg caagcacggg atgcgcgagg tagccgtgcg
     1381 cctcggccaa atcgctgatc gcgtacgcct gggccatggc gctgcgccct aagccagcga
     1441 tctgcgggaa gatgaaccac atccaatggc tggtcttgcg gccgttccgg agctcggtta
     1501 atgcccgctg ataggtgcca ccactattct gcgctcggac gaaccgctca agatccatga
     1561 gcccgacgct agtctcgctg acacttgaac gcagccacca tccctaccaa aaccggtcgc
     1621 gaacaacacc ggatccggcc ggactcttgt tgttggtgac cactttcggg gcaaggaggg
     1681 accgggtaac ccggggccac ctagcgctcg gccccgcccg caaccgccgg aataatggag
     1741 atcttggtgc cctcggcaac ctttgcatcc aggccgccaa tgaaacgtac gtcgtcgtca
     1801 ccgacgtaga cattcacgaa gcgtcgcagg gcaccggcgt catcgatgat ccgccccctg
     1861 atgcccggat ggttggtctc caaggaatcc aagacctcgg caagagtgct gccatcggca
     1921 ctcacctcgc ccgcac
//
LOCUS       DATAOZ010000488         8407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2827445, whole genome shotgun sequence.
ACCESSION   DATAOZ010000488 DATAOZ010000000
VERSION     DATAOZ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827445"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1071
                     /locus_tag="VHR64_12595"
     CDS             91..1071
                     /locus_tag="VHR64_12595"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HEX3303715.1"
                     /translation="MSDRPRLRVWSLARMGLITTSLVGSMALGGGSLVALAQDASPAA
                     SPSGNCAPGTMSTGMATPTDMATPVAEPSGSEADEATTAAAQAFVDNAKACASDPDAL
                     ATLVTPNLVKTLGGYDSIEAAQKDGFFTDAPFGNATTGAVTSYDDGSVGIEVKYMQTE
                     YQAVQEEWILVQANGEWKLNNIHPETPEIDGDSAAVGVNLNETGDGQYSIEPNASSVV
                     QTDVIILQAISADTNKEPHMLVVFQVPEGMTAADVAAGKVSEQDTTFIGGVQGVMPGT
                     SQDLTLVNLPAGKYVLACFVTGPDGKPHAMNGMVTDFEVTAPEPVATPAS"
     gene            1147..3006
                     /locus_tag="VHR64_12600"
     CDS             1147..3006
                     /locus_tag="VHR64_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3303716.1"
                     /translation="MTQQSGPFGALFEEYKAGRVSRREFLRRATMLGVGAPVALFVVN
                     SVSPSVANAQDATPAAGGAAAPSAGTEGQTRGAGGELKILQWQGVTHFGLHTATGTKD
                     QLGASLVTEALINFLPDGTMIPTLAAEVPSIENGGVAQDLSTVTYKLKQGVLWSDGQP
                     FSADDVVFTWQWITDPANNAIDIKTYQAISNVEAIDPNTVKVTFAAPTLGWNVPFCGT
                     YLGSIYPKHVLEAGADAYSAFIQNPVGTGPYKVESFTENDQVVYVQNDNYREPNKPFF
                     QKVNLKGGGDSTSAAQAVLQTGDWDFAWNLQTEPQILQQLEANGKGTVKASPYSNVER
                     LLINFSDPNKEVNGEKSQKDTPHPFFSDLKVRQAFALATDRDTMANQFYLGGDLEPGA
                     KNILSGIAALESPNTSFEFNLDKAAALLDEAGWTLDGDTRKKDGVEMKVTYSTSINAV
                     RQKEQALNKQNWESIGIKVNLKQVDAGIFFDSAAGNEQNAQHFYVDLLMYTNGPSSTF
                     PVSYMQSWYGGKDGVNISQKANGWSGINESRYHSDEYDAMYDQVAAATDAEAAAALFI
                     QMNDYLINNQVIIPEVARAAEKYAILNTLNNDNVAGSLFEALYWNVANWNRVQ"
     gene            complement(3248..4714)
                     /locus_tag="VHR64_12605"
     CDS             complement(3248..4714)
                     /locus_tag="VHR64_12605"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX3303717.1"
                     /translation="MAPTNPFASATDIAAAIQDRIVSSRELLELYLDRVERFNPALNA
                     IVTLDANRARSEADAADAAIARGDQLGPLHGVPITIKDAIETAGMRTTSGAKMLAEYV
                     PAQDAVAVARLRAAGAIVFGKTNLPEFAGDAQTFNALFGTTNNPWDPERTPGGSSGGA
                     AAAVATGLTGMELGSDLGGSLRIPAQFCGVYTIKPTWGIVPMTGHIPPPPDAQRDVDV
                     GVIGPLTRSAADLDLALQIIAGPDAAAGVAWRLELPPPRSDSLSGYRIAVWLDDPYSP
                     VDGQTGAVYEQVIADLARAGAHLTTTPLPVPLGEMHDVAQRLIQSSMSAWLPEDYYQS
                     LVDRAAAAPADDRSSPIRWARNVTQSVRAFRLAEQERIRQKAAWADFFTQYDALLCPV
                     MPTPAIRHDHERDVDARRIVFNKTTIPYGDQFAWLQAFGVVHLPAVVAPVGLTATGLP
                     VGIQIVGPHLEDRTPIDLARRMERITGGFQAPPGFGAV"
     gene            complement(4777..5640)
                     /locus_tag="VHR64_12610"
     CDS             complement(4777..5640)
                     /locus_tag="VHR64_12610"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX3303718.1"
                     /translation="MFLLLSLAIVTIGAWALTVHQARTMDMPMGVVLRAASNSQSEPA
                     GSDPMDGMAMDAPSTSGMQSVASSGMSGMGPESWSWASFSAFLVAWTVMMAAMMFPAV
                     APLLLLYRSMASGRSGGHAPVWILAAGYLLVWTAAGAVAWTIVQVGIEIGNRLGAGTR
                     GTVAPLALGATLIVAGLYQLTPLKQACLRQCQSPIGFLMSHWQAGKSGAVRMGLQHGL
                     FCLGCCWALFAVLVAAGVMSLAWMLLLTLVVFAEKVIRPGAWSQKLTGSALVALGLLV
                     AAGAAAMPWPA"
     gene            complement(5705..6343)
                     /locus_tag="VHR64_12615"
     CDS             complement(5705..6343)
                     /locus_tag="VHR64_12615"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX3303719.1"
                     /translation="MTDTSAPAWSFEGEYFENCNCTVACPCIFSAEQPFTSMPTEGAC
                     EVAFAFHLDSGSYGSTRLDGLNVGLAARTPGPMIDGNWQVALYVDERASPAQQEALQA
                     IFSGAAGGTMGGFAPLIGEVLGIKTVPITYATNGKRRSVEIPGIMSLAVRPVPSAMGE
                     DVEMIARNAHPFAPEGVVMAVGDSGSTWSDHGMRWDNSGRNGHYAPIRWSNA"
     gene            6531..7322
                     /locus_tag="VHR64_12620"
     CDS             6531..7322
                     /locus_tag="VHR64_12620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3303720.1"
                     /translation="MDGQVAMVTGAANGIGRAIVERFVAEGMRVVAVDISAADLTEAT
                     ASYGDKVHPAVADISDRTQVQAAVHAALFRFGQLDVLAANAGIADGEPFLEIDDKSWR
                     RIIDVNIHGTFHCVQEAAKVMATARKGAIVCTSSTNAWYVEKNLAHYNTSKGGVEALV
                     RSAAIDLAEFNIRVNAIEPSMVKTRAAFITEDPVGGPEYLKRVPMARFAEPAEIAAAV
                     AWLASGQSSYITGQTLILDGGLTLGIDMPLPAAPLPGSARAESDG"
     gene            7319..>8407
                     /locus_tag="VHR64_12625"
     CDS             7319..>8407
                     /locus_tag="VHR64_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3303721.1"
                     /translation="MIDRRRLLAAAAAPLILRSPARAQEPLRVLGWNGYLDQPTIDRF
                     KHATGIDVVLDIVGAYDEIFTQLRAGGIQRYSVIAPHHGLIGDLKQRGYIQPLDPNQI
                     PHLADIDSHFALPETTEISGEKYGAPLIFDTCPALYNADLLPEPPTTWLALASDQYTG
                     KVGMLDDGFSHMNLWGRAIGAAHPPNYSANEMLATVAILSELKQNRTSHFTPYPADLV
                     AQLANGKAVISTTGWGGLTLLPDRGKANIKVVHLQPGDFSFVQALAIPAEAPQVPAAH
                     QLIDFMLTPNEQAALANRTTRAIVNLAAVPMIDPAISAITNYADLDAVMAESPPLGFP
                     PLEESADGTASYLDWVLSWEKIRSLKSNA"
BASE COUNT         1609 a         2818 c         2608 g         1372 t
ORIGIN      
        1 atctgcggtg gacagttcaa tatgcccgga atgggcgccc gataccgtag tgacgttgcg
       61 tttggttcgt cggtaaaagg aggatcacgg atgtctgacc gtccccgctt gcgcgtgtgg
      121 agccttgcgc gcatgggcct gattactacg agcctggtcg gctcgatggc actcggcggc
      181 ggctcgctcg tcgcgctcgc ccaggatgcc tcgccggctg cgagcccatc cggcaactgc
      241 gcccccggca cgatgtcgac cggcatggcc actccgaccg acatggcgac gcctgtcgcg
      301 gagcccagtg gctcggaagc cgacgaggcc acgaccgccg ctgcccaggc attcgtcgac
      361 aacgcgaagg cctgcgccag cgatcctgac gcgctagcca cactcgtgac cccgaacctc
      421 gtcaagacgc tcggcgggta cgactcgatc gaggctgccc agaaggatgg cttcttcacc
      481 gacgcgccat tcggcaatgc cacgaccggc gccgtgacct cctatgacga cggcagcgtc
      541 ggtatcgaag tcaagtacat gcagaccgag taccaggccg tccaggagga gtggatcctc
      601 gtccaggcga acggcgagtg gaagctcaac aatatccacc cggagacgcc ggagatcgac
      661 ggtgacagcg ccgcggtcgg agtcaacctc aatgagaccg gcgacggcca gtacagcatt
      721 gagccgaatg cctcgtccgt agtccagacc gacgtgatca tcctacaggc catcagcgcg
      781 gacaccaaca aggagccaca catgctcgtg gtcttccagg tgcccgaagg catgaccgcc
      841 gctgacgttg ccgctggcaa ggtgagtgag caggacacga cgttcatcgg tggcgtccag
      901 ggcgtcatgc cgggcacctc gcaggacctg acgctggtca acctgccggc cggcaagtac
      961 gtcctcgcct gcttcgtcac ggggccagat ggcaagccac acgccatgaa cggcatggtc
     1021 acggacttcg aggtaacggc gccggagcca gtggcgactc cggcctcata acgcggaata
     1081 tccgccggcg gtcaatcgcg atcgccggcg atgttcacat agtttgacat tcaggaggaa
     1141 cagtcgatga cgcagcagtc cggccccttc ggggccctct ttgaagagta caaggcgggt
     1201 cgtgtctccc gccgcgaatt tctgcgccgc gcgaccatgc tcggggtcgg cgccccggtc
     1261 gcgctgtttg tcgtcaattc cgtctccccg tccgtcgcaa atgcccagga tgccacgccg
     1321 gcagccggtg gcgcggccgc tccgagcgcc ggaaccgagg gacagacccg tggcgccggt
     1381 ggcgagctca agatcctcca gtggcagggc gtcacgcact tcggccttca caccgccacc
     1441 ggcaccaagg accagctcgg cgccagcctc gtcaccgagg ctctgatcaa cttcctgccg
     1501 gacgggacca tgattccgac cctcgccgcc gaggttccct cgatcgagaa cggcggggtt
     1561 gcccaggact tgagcaccgt cacctacaag ctgaagcagg gcgttctctg gagcgacggc
     1621 cagcccttca gcgccgacga tgtcgtcttc acctggcagt ggatcaccga tccggccaac
     1681 aacgcgatcg acatcaagac ctatcaggca atcagcaacg tcgaggcgat cgacccgaac
     1741 accgtcaagg ttaccttcgc tgcgccgacg ctgggctgga acgtcccgtt ctgcggcaca
     1801 tacctcggat cgatctaccc gaagcacgtg cttgaggcag gcgccgatgc ctacagtgcc
     1861 ttcatccaga acccagtcgg cacgggaccg tacaaggttg agtccttcac ggagaatgac
     1921 caggtcgtct acgtgcagaa tgacaattac cgcgagccga acaagccgtt cttccagaag
     1981 gtcaacctca agggcggcgg cgactccacg tcggccgcgc aggccgtcct tcagaccggt
     2041 gactgggact ttgcctggaa tctccagacc gagcctcaga tcctccagca actcgaggcc
     2101 aacggcaagg gcacggtcaa ggcaagcccg tactcgaatg tcgagcggtt gctaatcaac
     2161 ttctcggatc caaacaagga agtgaacggc gagaagtcgc agaaggacac gccgcatccg
     2221 ttcttctccg acctcaaagt ccgccaagcc ttcgcgttgg cgaccgaccg tgacacgatg
     2281 gccaaccagt tctacctcgg cggtgacctg gagccgggcg ccaagaacat tctgagcggt
     2341 atcgccgcgc tcgagagccc gaacacctcg ttcgagttca acctcgacaa ggcagcagcc
     2401 ctcctcgacg aggccggctg gacgctcgac ggcgataccc gcaaaaagga cggcgtcgag
     2461 atgaaggtca cctactcgac ctccatcaac gcggtccgcc aaaaggagca ggccctcaac
     2521 aagcagaact gggagtcgat cggcatcaag gtcaacctca agcaggtgga cgccggcatc
     2581 ttcttcgaca gcgctgcggg caacgagcag aacgcgcagc acttctatgt tgacctgctc
     2641 atgtacacaa acggcccgtc cagcaccttc ccggtgtcct acatgcagtc gtggtacggc
     2701 ggtaaggatg gcgtgaatat cagccagaag gcgaacggct ggtccggcat caacgagtcc
     2761 cgctaccaca gcgacgagta cgatgccatg tacgatcagg tagccgcggc aaccgacgcc
     2821 gaggcggcgg cggcgctctt catccagatg aatgactacc tgatcaacaa ccaggtgatc
     2881 attccggagg ttgcgcgcgc ggctgagaag tacgcgatcc tgaacacgct caacaatgac
     2941 aatgtggcgg gctcgctctt cgaggcgctt tactggaacg tcgcgaactg gaaccgcgtc
     3001 cagtaatcgc ggaccaaaca agcgacagaa cagggtggcg aagtctcgcc accctgtttt
     3061 tgtagtgagg acacaccggc tccgtcccca actttctcgg tgccgcccgc ggctaaaggc
     3121 gagggctgag tcacgataag gcccttcggg actgattgag aagccggcta tagccgcctt
     3181 gtttctgctc agacccggtc ttcagcccgg ggcggctcgg acgcacgatc ccaccgaact
     3241 gttgtagtca aaccgcgccg aatcccggcg gagcctggaa gccgccggta atccgttcca
     3301 tgcggcgggc aagatcgatc ggcgtacgat cctccagatg tggcccgacg atctggatcc
     3361 cgaccggcaa tccggttgcg gtcaaaccga ccggcgcaac gaccgctggc aaatgcacca
     3421 caccgaatgc ctgcagccac gcgaactggt ccccgtatgg gatcgtcgtt ttattgaata
     3481 cgatgcgtcg cgcatccaca tcccgctcgt ggtcgtgccg gatagcggga gtcggcatca
     3541 ctgggcagag cagggcatcg tactgggtga agaaatccgc ccatgctgcc ttctgccgga
     3601 tccgttcctg ctcggccaag cgaaatgccc gcacgctctg cgtgacattg cgtgcccacc
     3661 gaatcggcga gcttcgatca tccgccggcg ccgcggctgc ccggtccacc agtgactggt
     3721 agtagtcctc gggcagccat gctgacatcg acgactggat cagccgctgc gccacgtcgt
     3781 gcatctcacc aagtggcacc ggcaaaggcg tggtcgtgag atgtgcgccc gccctggcga
     3841 ggtcggcgat cacctgctca tagaccgcgc ccgtttggcc gtcgaccggg ctgtagggat
     3901 cgtcgagcca gaccgcgatt cggtacccgg acaacgaatc cgaccggggc ggtggcagct
     3961 cgagccgcca tgcgacgcca gccgcggcat ccggcccagc gatgatctgc agggccaggt
     4021 cgagatccgc agccgacctc gtcaatggtc cgatcactcc gacgtcgaca tcgcgctggg
     4081 cgtccggcgg cggcggaatg tgaccggtca tcggcacgat gccccaggtc ggcttgatgg
     4141 tgtagacgcc gcagaattgg gccgggatcc gcaaggaccc gcccagatcg ctgcccagct
     4201 ccataccggt caggccggtt gcgaccgccg cggcagctcc gccggacgaa ccaccgggcg
     4261 ttcgctccgg atcccacggg ttattggtcg tgccgaaaag cgcgttgaac gtctgtgcat
     4321 cgccggcaaa ctcgggcaga ttggtcttcc cgaagacgat cgccccggcc gcccgcaacc
     4381 tggccaccgc aacggcatcc tgcgctggca catactcggc cagcatcttc gcaccggatg
     4441 tcgtccgcat gccggcagtc tcgatcgcgt ccttgatcgt aatcggcacg ccgtggagcg
     4501 ggccgagttg gtcgccgcgg gcgatcgccg cgtcagctgc gtccgcctcc gaccgggcgc
     4561 ggttggcatc gagtgtcacg atcgcattga gtgccgggtt gaaccgctcg acgcgatcaa
     4621 ggtagagctc cagcagttcc cggctggaga cgattcgatc ctgtattgcc gcggcgatgt
     4681 ccgttgccga cgcgaacgga ttcgtcggag ccatggagcc tccatctacg cttcgaggac
     4741 cgggcgcacg cacgcctgat ccgggtgagt ggctggtcat gctggccagg gcatggcggc
     4801 ggctccggcc gccaccagca atccaagcgc aactaacgcg gaaccagtca gtttctggct
     4861 ccacgctccc gggcgtatca ccttctccgc gaagacaacc agggtcagca gcagcatcca
     4921 ggcaaggctc atcacgccgg ccgcaaccag aacggcaaac agtgcccagc agcagcccag
     4981 gcagaaaagc ccgtgctgga gccccatgcg cacggccccg gacttcccgg cctgccaatg
     5041 gctcatcagg aatccgatcg gcgactggca ctggcgcagg caagcctgct tgagtggcgt
     5101 gagctggtac agcccagcca cgatcaatgt cgctccgagt gcgagcgggg cgacggtgcc
     5161 gcgagtccct gcaccgagcc ggttgccgat ctcgattcca acctgcacga tcgtccaggc
     5221 aacagccccc gcagcggtcc agacgagcag gtatccggcc gccaagatcc agaccggtgc
     5281 gtgaccgccc gacctgccgc tggccatcga ccgatacagg agaagcagcg gcgccaccgc
     5341 cgggaacatc atggccgcca tcatgaccgt ccaggcgacg aggaacgcgc tgaaactcgc
     5401 ccagctccag ctctccggcc ccatcccgga cattcctgag ctggcaacgg actgcatccc
     5461 ggacgtggac ggcgcatcca ttgccatccc atccatcggg tctgatccgg ccggctcgga
     5521 ctgcgaattg ctcgcggccc gcagcacgac gcccatcggc atgtccatcg ttcgggcctg
     5581 gtgcacggtc agcgcccatg caccgatggt gacaatcgcc agcgagagca gtaggaacat
     5641 gccaccccgc ccaggggcag agcgaccgga ccgcctcagc ccgacaccaa catctgccgt
     5701 ggcgtcaggc attggaccag cggatcggcg catagtgacc gttccgcccg gagttgtccc
     5761 agcgcatgcc gtggtcggac cacgtgctgc cggaatcgcc caccgccatg accacgccct
     5821 ctggcgcgaa cgggtgcgcg tttcgcgcga tcatctccac gtcctcgccc atcgcgctgg
     5881 gaacgggtcg aaccgcgagg ctcatgatgc cggggatttc gaccgatctt cgcttgccat
     5941 tggtcgcgta ggtaatcggc acggtcttta tgccaaggac ttcgccgatc agcggggcaa
     6001 atccgcccat cgtgccgccg gccgcgccgc tgaagatcgc ctgcagcgcc tcctgctgtg
     6061 ccggacttgc gcgctcatca acgtagaggg cgacttgcca gttgccgtcg atcattggcc
     6121 ccggcgtgcg cgcagccagc ccgacgttca ggccgtccag ccgcgtggac ccgtatgatc
     6181 cggagtcgag atgaaacgcg aacgccactt cgcaggcgcc ctccgtcggc atcgaggtga
     6241 atggctgctc tgccgaaaag atgcatgggc acgccacagt gcaattgcag ttctcgaaat
     6301 actcgccctc gaacgaccag gctggtgcgc ttgtgtcggt catcaaagtt cctcccggat
     6361 cggaacagaa cgcgacagga cggggggcag gagttgaaat cgacggaacg tatccggtat
     6421 agcgacgttt gttagtgatg tcaactacgg gagtgttcgg taggctctta ccagcgtgat
     6481 gcgatcggcg atgatttggg acagatgaga ggagtgattt cgtgctgcag atggatggtc
     6541 aggtcgcgat ggtgaccggg gccgcaaatg gcattggtcg ggcgatcgtc gagcggttcg
     6601 tcgccgaggg gatgcgcgtc gtcgcagtcg acataagcgc cgcagatctg actgaagcga
     6661 cggcatcgta cggagacaag gttcacccgg cagtggccga tatctcggat cgtacgcagg
     6721 tgcaggcggc cgtgcatgcc gcgctcttcc ggttcggcca gctcgatgtg ctggcagcca
     6781 acgccgggat cgccgatggc gagccgttcc tcgagatcga cgacaagagc tggcgccgga
     6841 tcatcgacgt caacatccac gggaccttcc actgcgtcca ggaggcggcg aaggtgatgg
     6901 cgacagcgcg gaagggcgcg atcgtctgca cctcatcgac caacgcctgg tacgtcgaga
     6961 agaatctggc ccactacaac acgtccaagg gcggggtcga ggcgctggtc cgctcggcgg
     7021 caatcgacct ggccgagttc aacatccgcg tcaacgcgat cgagccgagc atggtcaaga
     7081 cccgcgccgc cttcatcacg gaggatccgg tcggcggtcc cgagtatctc aagcgggtgc
     7141 cgatggcccg gttcgcggag ccggccgaga tcgccgcggc ggtggcctgg ctcgcctccg
     7201 gccagtccag ctatatcacc gggcagacgc tgatcctcga tggcggcctg acactaggga
     7261 tcgacatgcc actaccggct gcgccgctgc ccggttcggc ccgcgccgaa tcggacggat
     7321 gatcgaccgc cgtcgtctcc tcgccgcagc ggctgctccg ctgatcctgc gctcgccggc
     7381 gcgggcacag gagccgctgc gcgtgctggg atggaacggc tatctcgatc agccgacaat
     7441 cgaccggttc aagcacgcga ccggtatcga tgtcgtgctc gacatcgttg gcgcgtacga
     7501 cgagatcttc acgcagctgc gggcgggcgg catccagcgg tattcggtca ttgccccgca
     7561 tcatggtctg atcggtgacc tcaagcagcg cggctacatc cagccgctcg acccgaatca
     7621 gatcccgcac ctggccgata tcgactcgca tttcgccctg ccggagacga ccgagatcag
     7681 tggggaaaaa tacggggcgc cgctgatctt cgatacctgc ccggccctgt acaacgccga
     7741 tctgctgccg gagccaccga cgacttggct ggcgctggcc tccgaccagt acaccggcaa
     7801 ggtcgggatg ctggacgacg gcttcagcca catgaatctc tggggccggg ccatcggcgc
     7861 ggcgcatcca ccgaactact cggccaacga gatgctggca accgttgcga tcctctccga
     7921 gctcaagcaa aaccgcacct cgcacttcac cccgtacccg gccgatctgg tcgcccagct
     7981 tgccaacggc aaagcggtca tctcgacgac cgggtggggc ggactgactc tgctgccgga
     8041 tcgcggcaag gccaacatca aggtcgtgca cctccagccc ggcgatttca gcttcgtgca
     8101 ggcgctggcc atcccggccg aggcgccgca ggtcccggcc gcgcaccaat tgatcgactt
     8161 catgctcacg ccgaacgagc aagccgcgct cgcgaaccgc acgacgcggg cgatcgtcaa
     8221 tctcgccgcg gtgccgatga ttgacccggc catcagcgcc atcaccaact acgccgacct
     8281 cgacgccgtc atggcggaat cgccgccgct cggctttccg ccgctcgagg aatcggccga
     8341 cggcaccgcc tcctacctcg actgggtcct gtcgtgggag aagatccggt cgttgaaaag
     8401 caatgcg
//
LOCUS       DATAOZ010000489         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7565798, whole genome shotgun sequence.
ACCESSION   DATAOZ010000489 DATAOZ010000000
VERSION     DATAOZ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7565798"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VHR64_12630"
     CDS             complement(<1..1201)
                     /locus_tag="VHR64_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX3303722.1"
                     /translation="MEVAAWTSLHRLVTAQEERRPLSRVTLKRVASFARPMRRQLVQF
                     LLLSIVSAVLAVATPVLAGRVVDAIVKGESERTVITLALLIAGIAIADAGLGIITRWL
                     SANIGEHLILDLRIAVFDHVQRMPIAFFTRTRTGALVSRLNNDVIGAQRAFSDTFSGI
                     VSNVVMLLLTLIVMLRISWQITLLALILLPIFVLPARKMGSRLAALRTEAANHNAEMS
                     TQMTERFSAPGATLVKLYGRPAEESAEFAKRANRVRDIGVRSAMLQWIFVTALTLVSA
                     LALAVVYGLGGYYALQGTLEAGSVVALALLLTRLYAPLTALANARVEVMGALVSFDRV
                     FEVLDLQPLIVEKPDAQEVPPGPVAVEFDHVRFSYPSADKVSLASLESVAVLDTRGGV
                     EVLHDISF"
     gene            complement(1360..>1805)
                     /locus_tag="VHR64_12635"
     CDS             complement(1360..>1805)
                     /locus_tag="VHR64_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303723.1"
                     /translation="SDADLYPVPDPVDLPAIRRAQELAVIRPIARNVPIAGGRYAETL
                     TIGPYETRCLWITPSSPLIPDPPAWIAVDRRTDRTIVRWEASREPWFWSYELYRMDRD
                     NPVDRLTPDPLRSASWTDARPGWGKASYGVRVVCASGVPGPLAIA"
BASE COUNT          332 a          599 c          602 g          272 t
ORIGIN      
        1 ggaatgagat gtcatgcagc acctcgactc cgccccgcgt atcaagcacg gcgaccgact
       61 cgagcgacgc cagcgagacc ttgtcggccg acggatagct gaaacggacg tggtcgaact
      121 cgaccgctac cggtcccggc ggcacctcct gtgcgtccgg cttctcgacg atcagcggtt
      181 gcagatcgag cacctcgaat acgcgatcga agctgaccag ggcgcccatc acctcgaccc
      241 gggcattggc cagcgcggtc agcggtgcat agagtcgcgt cagcaacaag gccagcgcga
      301 cgaccgagcc tgcctcgagc gtgccctgca acgcgtagta cccgccgagg ccgtagacca
      361 ccgccagggc gagcgcggac acgagggtca gcgcggtcac gaatatccat tgcagcatcg
      421 cggaccgcac gccgatgtcg cggacacgat tggcccgctt cgcgaactca gccgactcct
      481 ccgccgggcg gccgtagagc ttgaccagcg tcgccccggg tgcggagaag cgctcggtca
      541 tctgggtgct catctcggcg ttgtggttgg cggcctcggt ccgcagcgcg gccaggcggg
      601 agcccatctt gcgcgccggc agcacgaaga tcggcagcag gatcagcgcc agcagcgtga
      661 tctgccacga aatccgcagc attacgatca gggtcaggag cagcatcacg acattgctga
      721 cgatgcccga gaaggtgtcg ctgaaggccc gctgcgcgcc gatcacgtcg ttgttgagcc
      781 ggctaaccag cgcgccggtc cgggtccggg tgaaaaaagc aatcggcatc cgctggacgt
      841 ggtcgaagac ggctatgcgc agatcaagga tcaggtgctc gccgatgttg gccgagagcc
      901 agcgagtgat gatccccaga ccggcgtcgg cgatcgcgat cccggcgatc agcagggcca
      961 gcgtgatcac cgtccgctcc gattcgccct tgacgatcgc atcgaccacg cggccggcca
     1021 gcacgggcgt cgccacggcc agcacggccg agacgatgct gagcaacagg aactggacga
     1081 gttgccggcg catgggccgg gcaaacgagg cgaccctttt cagcgtgacg cgcgagagcg
     1141 gccggcgctc ctcctgcgct gtcaccaacc gatgcaatga ggtccaggca gcaacttcca
     1201 tctatatctc cacgcagcac cgatgctccg ccaatttcaa tctcaaatcc aatatttgac
     1261 cccgtgtcgg ggcatgctgt tgatcgtagc gcgagtctcc gttgaccggc ataccgcaat
     1321 cgggtaggtt cggtcaccgg gaccgatggt gggacgcatt caggcgattg ccagtggacc
     1381 aggcacgccc gaggcgcaca ctacccgtac accatacgac gccttgcccc aaccgggccg
     1441 cgcgtccgtc cacgatgcgg atcggagggg gtccggggtc aggcgatcga ccggattgtc
     1501 ccggtccatc cggtagagct cgtagctcca gaaccacggc tcgcggctcg cctcccagcg
     1561 cacgatggtc cggtcggtgc ggcggtcgac ggcgatccag gccggcggat cggggatcag
     1621 cgggctggat ggcgtgatcc agaggcagcg cgtctcgtac gggccgatcg tcagcgtctc
     1681 ggcatagcgg ccgccggcga tcgggacatt gcgggcgatc ggccggatga ccgcgagctc
     1741 ctgcgcccgc cggatcgccg ggagatcgac tgggtcaggc acggggtaca ggtcggcatc
     1801 cgatc
//
LOCUS       DATAOZ010000490        12331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1511058, whole genome shotgun sequence.
ACCESSION   DATAOZ010000490 DATAOZ010000000
VERSION     DATAOZ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12331
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511058"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VHR64_12640"
     CDS             complement(<1..337)
                     /locus_tag="VHR64_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303724.1"
                     /translation="MRSGHRATNEPHTRKTWRWLLVLGPGLLAAIALVAGFQRSSGGD
                     FWATLYDEVVSTPIYLPLGVFGMIRWSLWLCKKIPAAFYQPITAPYTTSAAIVTPVYQ
                     EDPVIFRRAL"
     gene            complement(370..1425)
                     /locus_tag="VHR64_12645"
     CDS             complement(370..1425)
                     /locus_tag="VHR64_12645"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303725.1"
                     /translation="MARITVVGSGVVGQASGRGFLHHGHEVTFVDINPEIIERLRIEG
                     LTARFPDEVHWADADITMIAINTPTVDGKIRLEHFLNGLRTVAEGLRGSTRFHIVVVR
                     STVPPTTTVNTVLPLLQALSGRRVGDTLGLAMNPEFLRQISANQDFLEPWVTVFGTYG
                     DAEAAILGELYAPFNAPIVAVDCTTAEVIKYANNLYNATKISFFNEFHLVCEQLGIDS
                     LVVGRTISRTAEGMWNPAYGTRGGWPYGGACLPKDTAAFYEFATEKLGLEMPVLLGTM
                     RVNEILTQRTAPPTTMHEVVPADELILDAHASANHAATVAISGALSSEFVDPPAEQML
                     NAPITLAHPGGEEIVAP"
     gene            2223..2660
                     /locus_tag="VHR64_12650"
     CDS             2223..2660
                     /locus_tag="VHR64_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303726.1"
                     /translation="MHPSLHVRRRLAILAILALIFPIWSPPVVPVAAQDGLVTEKVLT
                     DVIIQPPAPDGSLTVTRLILDSASRTQSMVFETSIVVVVETGEIKLWTRAGTTIDGIP
                     VTSSPATVFVERSQTIIVPGGVRFRVRAWGCGPARLLFITLTG"
     gene            2773..3300
                     /locus_tag="VHR64_12655"
     CDS             2773..3300
                     /locus_tag="VHR64_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303727.1"
                     /translation="MKRAVSLLSLALGLLVGGLLGTGTVNIRAQDATNCATPVDEGQV
                     TSSQRMLGRSDYSQLPAAPARLTTTQITLAPGGATQPFASTGPVLVVVQEGTVTLTAD
                     LARVGQPPEPSLGGIQVEGDTAPPAPADGVGVTKGEQISLESGVTAKFGNDTSAPVRL
                     LLVTLAPGEAGTPTP"
     gene            complement(3319..4746)
                     /locus_tag="VHR64_12660"
     CDS             complement(3319..4746)
                     /locus_tag="VHR64_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3303728.1"
                     /translation="MFHLPSFCPECGTRLSPADHVCPQCGTQVLPDPIPEPVILERPR
                     ATKVRELVAVITIFSIMVAIIMRSYASSQGEEPTRTPAAVAAVTVTPTAIPELLALGS
                     VPPKVTSETTASFSYTWTDPSGGQFECRLDAEQFTSCDPDGKQYEQLPQGIHTFQVRV
                     IRPNGVPTNAVSYGWRIDFSVPELSFFPDGGTYATKELVVIQSSKPGKIYYTTDGSDP
                     TTASTAYTDPILVQSTTTIKAVTIDDTGNASKIASVSYAFQPEFHDDFEDGSLANWSN
                     VTNLIIDDQGGLNSQRAARAISTDGTPAWASIALSQPVNDLYVQAAVLLRSRGGNPVA
                     LMRIRDSNDNVLVTLYVTTTGKLGVLYGTTASATTGLVVRPGQWNEIELHVSVVAGRT
                     VVEVWYNGTYIEDLHLTSALVASPLSTLQIGESASKRLFDISYDNVITDTTFIPSAFL
                     VVESGPNGSTPIAVGEPQASPAAQP"
     gene            4946..6535
                     /locus_tag="VHR64_12665"
     CDS             4946..6535
                     /locus_tag="VHR64_12665"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX3303729.1"
                     /translation="MRGRYLFLTFAIAVLVAIPQATLADSNSDSDLTSALSVLSKSRI
                     APLGRLSGSAPDGMPFRRVDGTNGIGSVILPRRTDPYQLPELAKLFPDSIVPSGDGIY
                     LLNDDLIVGQGATLLIESRTVHEVRLRSMPDHFITITSWRGTITVAGTEYQPVAITSW
                     NPNENGPDLNEADGRAWVQTRRGSMDLTWANTSYLGFDTGTLSGVAWEGRPDEQVHGS
                     AIYSTFMHNHFGAYTFEATAMEFRYDTFAYNTGYGLDPHDHSNHFTVEYNRAYNNGTH
                     GIIFSRGCDFNVIRFNASYNNGTHGIVLDDGPNVNPDGTIRERAGIPSNNNSVTQNTV
                     YGNQIGIVLDGGTGNTISGNQIRQNTYGIRMKDAVDENAVTGNQFIDNTQFAIYLYNG
                     SNRNVIAHNTVSGGTSGIVIKDSASNQVDSNTLKGISDRGIAFIGKVSESEITNNNAD
                     QVSTPLQLPDVAVSGPVTMQGNSFDASRPSDTHPRSIHAPAVRWLIWAFLLLFPVLFG
                     SRVLRLLHNVRMSRPFAMRPK"
     gene            6672..6977
                     /locus_tag="VHR64_12670"
     CDS             6672..6977
                     /locus_tag="VHR64_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303730.1"
                     /translation="MAQQTRFWLYRAGTPVHSTLGELIGHVVAVRPDAVLVAGDSGHE
                     RWFPASEIAEFSDRGLIVCVSQEIAWNLPPTASPRPDDRHVPDSRLFLRDPLNRYDA"
     gene            7248..8471
                     /locus_tag="VHR64_12675"
     CDS             7248..8471
                     /locus_tag="VHR64_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303731.1"
                     /translation="MSHPQAARAIRAFFVVALIGAFAVHVEAPRPVSAAATLVSMSAS
                     GSATGGKRVHIRVVISEPAPVGGLDIPLSSNNPAVPVPSHAHVNSGATEELVGVTTRP
                     VVDTVIVTVTASYKGVVKSRPITIKEPIHSSLSLQSVIRAGGQGRVTVRLSGRAPVGG
                     ITVQLSTDRSDKFALPTSVTVLEGAASAILKVNATDQVPDVPISVTSHYQKGSINNTY
                     TKNAIIRHFTATPTPTATNPTDVPSITPTWTMTPTATATPDPNNPTFSFTVKSGDPVV
                     SGGSVVLTVCMLTGPNPPYTITFISTSQQRAMVTAGSPHTYAALNECTDVTLTDLPYP
                     TSGNVRLRARLSPSNVTVGESANINFPGEPLTATPTSTTAAAQSANQPAQPTSTPVPT
                     EPSEPILLLSPEGTP"
     gene            8674..8979
                     /locus_tag="VHR64_12680"
     CDS             8674..8979
                     /locus_tag="VHR64_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303732.1"
                     /translation="MSTDHAYPERRDLDAQGNLLSAAQEGHRHPWESRLNGDELASMP
                     YLEPGTRLQDGQSYLDLNQLNGGPFTPSGGRSVQAGDRIVAQRDLDPDLWRKLVGEG"
     gene            9019..9504
                     /locus_tag="VHR64_12685"
     CDS             9019..9504
                     /locus_tag="VHR64_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303733.1"
                     /translation="MSDDYSPGDARKPYSDIGGPGAKHAQTHDVRREQLTNPKGSEPV
                     DTSFAAQTEPGTPWEPGIHADESSPAVDDKNLHDRLPQLTNDELARLPVLDADTQLEQ
                     GSVYLDLNQLDKGPFRTLARSASRNERLIAKQQTDYLLWNRLAIEDADPTVERPEGAG
                     M"
     gene            9501..9995
                     /locus_tag="VHR64_12690"
     CDS             9501..9995
                     /locus_tag="VHR64_12690"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3303734.1"
                     /translation="MTEPGEPPHSHGLGTPETTGTIEIVRQFKAPPSAVFRAWTDPAV
                     LTRWLAQKAESDPRMGGHYRLETDEPGDVLEPHVCSGEYREFVPDQRLVMTWIYNGPH
                     PEDLAESLISIDFAPIDGDTTEIHFREVGEGLATDEERDYSRTAWQGAFNNLDRAIIE
                     SHTV"
     gene            complement(10038..11894)
                     /gene="typA"
                     /locus_tag="VHR64_12695"
     CDS             complement(10038..11894)
                     /gene="typA"
                     /locus_tag="VHR64_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX3303735.1"
                     /translation="MEAPIAAPSAPVSAAQLRNVAIIAHVDHGKTTLVDGLLKQSHIF
                     RDPDAAGELIADSNDLERERGITILAKNMAITYRGLKINIIDTPGHADFGGEVERVLN
                     MADGCLLLVDAVEGPMPQTRTVLQRALALGLTPVVVVNKIDRPAARCDEVVELTHDLF
                     LDLATDPDQLDFPIIYAIAKQGRAGFAPDQLQEDLRPLLDTIVDTIPAPKVAAEAPVQ
                     MLVGSLDYDTHRGRIAIGRVHRGVIRAGETLIHIAPDGTETRQRITDLATFEGLGKRR
                     VDEAIAGDIVAVAGFADAKIGATLADPNAPEALPTIEIEEPTLKLTFGVNTSPFSGKE
                     ATYSTSRQLRERLYRELETNLSLRVEPTDQADVFAVSGRGELHLSVLIETMRREGYEF
                     QVSRPEVITREIDGVTMEPVEHLVIETVEDSVGVIMDLVGTRKARMLDMVNDGRGSVR
                     LEFAIPTRGLIGLRNAFLTATKGNAVMGSRLIGYEPWLGTIPNNRTGALVASEPGQVL
                     AHGVANAQERGITFVEPGEDIYMGQIVGQQPRQGDMVVNVCRAKKLTNMRASTADIAI
                     KLVPAVKLSLEQSLDFLADDELLEVTPKAYRLRKRLLNESDRAKERKRLSQS"
     gene            complement(12047..12253)
                     /locus_tag="VHR64_12700"
     CDS             complement(12047..12253)
                     /locus_tag="VHR64_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303736.1"
                     /translation="MNINSARSEVEKTAGIFRHLVSPDHPTDIDAIIEIENRGFEEAI
                     IQDWIETERRMRPDYVPEDETDQA"
BASE COUNT         2267 a         4001 c         3830 g         2233 t
ORIGIN      
        1 cgagtgcgcg ccggaagatg accggatctt cttggtagac cggagtcacg atcgcggcgc
       61 tggtcgtgta cggcgcggtg atcggctggt agaaggccgc cgggatcttc ttgcagagcc
      121 agagcgacca ccggatcatg ccgaagacgc ctagcggcag atagatcggc gttgagacga
      181 cctcgtcgta cagcgtcgcc cagaaatccc caccgctgga gcgttgaaat cctgcgacga
      241 gcgcaatggc tgcgagcagc cccggtccga gcacgaggag ccaccgccac gtcttccggg
      301 tgtggggctc gttggttgcg cggtgcccgg acctcatctg cggggatctc ctctgctccg
      361 gcccgctccc tagggagcga cgatttcctc accccccggg tgtgccagag taatcggggc
      421 attcagcatc tgttcggctg ggggatcgac gaactcggag ctgagcgcgc cactgatcgc
      481 gaccgtggcg gcatgattgg ccgaggcgtg cgcgtcgaga atcagctcat ctgccggtac
      541 gacctcatgc atcgtggtcg gaggtgcggt gcgctgcgtc aggatctcgt tgacgcgcat
      601 cgtgccgagc aggacgggca tctccagccc aagcttctcg gtggcgaact cgtagaacgc
      661 ggccgtgtcc ttgggcaggc aggcgccgcc gtatggccag ccgccgcgcg tcccgtacgc
      721 cgggttccac atgccctccg ccgtccggga gatggtccgg ccaacgacca gggaatcgat
      781 gccgagctgc tcgcaaacaa ggtggaactc gttgaagaac gagatcttgg tcgcgttgta
      841 gaggttattg gcgtacttga tgacctcggc cgtcgtgcag tcgacggcca cgattggcgc
      901 gttgaacggc gcatagagct cgcccaggat ggcggcctcg gcgtcgccgt aggtgccgaa
      961 cacggtaacc catggctcca ggaagtcctg gttcgcgctg atctggcgga gaaactcggg
     1021 attcatggcc agaccgagcg tgtcgccgac acggcggccg gaaagtgcct ggagcagggg
     1081 caggaccgtg ttgacggtcg tggtcggcgg gactgtgctg cgcaccacga cgatgtggaa
     1141 tcgcgtagag ccccgcaacc cctcggcaac cgtacggagg ccgttcagaa aatgctcgag
     1201 ccggatcttg ccgtcaactg tgggcgtgtt gatggcgatc atggtgatgt cggcgtcggc
     1261 ccagtggact tcatcgggga acctggcggt gaggccctcg atgcggagac gctcgatgat
     1321 ctcgggattg atgtcgacga aggtgacctc gtgtccgtga tgaagaaatc cgcgtcccga
     1381 tgcctgtcca accacaccgg atccgacgac agtaattcgt gccatgtcct ctcatctcct
     1441 gtcgcaagag gagctcgttc cgcgtgggga gatcgggtgt gatcgggtcg cggtacgagc
     1501 aacggagtca gcctgcgcga ttggcggatg cgtcgcccgg acaggagcga gtcgctgtcg
     1561 ggaggcgcgg ccaatgctgt gacggaagaa gaagcgctcc tttcgcatgc gggcagagtc
     1621 tgattcaggg cgtgcccggc cagaaggccc agccacggcc tttcatcaca tgcaacttgg
     1681 tccgaatgtg gtgctgtggc gaggttggta ctcgcgatgc ctcagctgtg gttcttgcct
     1741 tgacttgtca cctcaccatg gcacacacca tggcggacca gcagatcccc caagcccacg
     1801 ctgccgcgga ttccatccaa tctgcggttg aatccaccag cgccttggtc ttgcgtcaac
     1861 aatgacgcga cgttttcggc acggccatgc tagtcccgat acctcccgaa cgcaactgta
     1921 cggacagaac aatttgctac aaaattagta atcattgctg accgatgcta caaatccgta
     1981 tcgttgcctg gaatgcacac gctatccagc tgccgtgtcc aacagtacgg atgcttccac
     2041 cgattggttt tccggacaga aggtgggccg ggctttcggg ctgaggcgtg gtgtggtaga
     2101 agtgaaccta cgtcataccc gctccgacga cctgacccgt gccgctgcac ccgattcgcc
     2161 cgcacccggc gggcgaaacc gcaccgtccg gggtcgccca cgcactgaca accggaggga
     2221 atatgcaccc gtcactgcat gttcgtcggc gcctcgccat ccttgccatc ctcgcgctga
     2281 tcttcccgat ctggtccccg ccggtcgtgc ccgtcgccgc ccaggatggg ctggtcactg
     2341 aaaaggtgct gaccgacgtg atcatccagc cgccggcgcc cgacggctcc ctgaccgtca
     2401 cccgcctgat cctcgactcc gccagtcgca cccagtcgat ggtcttcgag acatcgatcg
     2461 tggtcgtcgt cgagacgggc gaaatcaagc tctggacccg ggccggaacc accatcgacg
     2521 gcatcccggt gacgtcgtca ccggcgacgg tcttcgtgga gcggtcgcag acgatcatcg
     2581 tgccgggcgg cgttcggttc cgggttcggg cctggggatg tggaccggcg aggctgctgt
     2641 tcatcacgct gaccggatga gcgccaggcg tatgctcggt gtcttgccag ggagggccca
     2701 ccagacgggc cagaacacga caggcggctc accaaccggg tggccgcagc cagaggaaac
     2761 ggagatcagg ctatgaagcg cgctgtttcg ctcctttcgc tcgcactcgg actcctcgtg
     2821 ggtggtctgc tcggcaccgg aacggtcaat atccgcgccc aggatgccac caactgtgcg
     2881 acgcccgtgg atgaagggca ggttaccagc agccagcgaa tgctcggccg ttccgactat
     2941 tcacaactgc cagcggcccc ggcgcgcctg acaactaccc agatcacgct cgcaccgggt
     3001 ggcgcgacgc agccgttcgc cagcaccggg ccggtcctgg tagtcgttca ggaaggtact
     3061 gtgacgctga cggcagatct cgcgcgcgtc ggccagccgc cggagccctc gctcggcggt
     3121 atccaggtcg agggcgacac cgcacctccg gccccggccg atggcgtggg cgtcaccaag
     3181 ggcgagcaaa tctcactcga aagcggtgtc acagccaagt ttggaaatga caccagcgcg
     3241 ccggtgcggc tgctcctggt taccctggcc ccgggcgagg cgggaacccc gacaccgtaa
     3301 tcgactcgcc gatcgcggtc agggttgcgc ggccggtgat gcctgcggct cgccgaccgc
     3361 gatcggcgtg ctcccgttcg gaccgctttc gacaaccagg aatgccgacg ggatgaacgt
     3421 cgtgtcggtg atgacgttgt cgtacgaaat gtcgaagagt cgcttgctcg cgctctcccc
     3481 gatctgcagc gtgctgagcg ggctggcaac gagcgccgat gtcaagtgga ggtcctcgat
     3541 ataggtgccg ttgtaccaga cctcgacaac cgtgcgcccc gccacaacgg agacgtggag
     3601 ctcgatctcg ttccactgtc ctggacggac gacgagtccg gtcgtggcag acgcggtcgt
     3661 cccgtagagg actcccagct tgccggtagt tgtcacatag agcgtgacca ggacgttgtc
     3721 gttgctgtcg cgaattcgca tcagggcgac cgggttgccg ccgcgcgagc gcaggagtac
     3781 tgcggcctgg acgtaaaggt cgttgaccgg ctggctgagc gcaatggagg cccatgcggg
     3841 tgtgccgtcg gtactgatcg ccctggctgc ccgttggctg ttcaggccgc cctggtcgtc
     3901 gatgatgaga ttcgtgacat ttgaccagtt cgccagagat ccatcctcga agtcgtcgtg
     3961 aaactctggc tgaaatgcgt agctgacgct ggcaatcttc gacgcatttc cggtgtcgtc
     4021 aatggtgacc gccttgatcg tcgtcgtcga ctgcacaagg atcgggtcgg tgtatgctgt
     4081 cgatgccgtc gtcggatcag atccgtctgt ggtgtagtag atcttgccgg gtttggagct
     4141 ctggatcaca acgagctcct tcgtcgcgta ggtgccgcca tccgggaaga agctcagttc
     4201 cggcaccgaa aagtcgatcc tccagccgta gctgaccgcg ttcgtgggga cgccgttcgg
     4261 ccggatcacc cgaacctgga acgtatggat cccctggggc agctgctcgt attgcttgcc
     4321 gtccggatcg cacgaggtga actgctcggc atccagccgg cactcgaact ggccgccgct
     4381 cgggtcggtc caggtgtagc tgaacgacgc agtagtttcg gatgtcactt tcggcggaac
     4441 agaccccagc gcgagcagtt ctggtatcgc tgtcggcgtg accgtgacag ctgccacggc
     4501 ggcgggggtc cgagttggct cctccccttg tgagcttgca tagctgcgca tgatgatggc
     4561 gaccatgatc gagaagatcg ttattacggc aacgagttcc cgcaccttgg ttgcccgcgg
     4621 tcgctcgagg atgaccggct cggggatggg atccggcagg acctgcgttc cgcactgtgg
     4681 gcagacgtga tcggccggag agaggcgagt gccgcactcc gggcagaacg agggcaaatg
     4741 aaacaactcg gatggtgaag ggctcgcgtc gcgtctcagg ggcgttccct accaatgacg
     4801 ggcatggtgg cccagcgggt atgtggcgac agcatagtcg ccgatccggg aatccgatag
     4861 tatactcggc ctctcggatc accagaacca atctgcgctt cccgcgcgta tctcattctg
     4921 cgtcgatcca ggcgaggagg ccccgatgcg tgggcgttat ctcttcctta cgttcgcgat
     4981 tgctgttcta gtcgccatcc cgcaggcgac gttagctgac agcaatagcg actcggacct
     5041 aaccagcgcc ttatccgtgc tcagcaagag ccggatagca ccccttggcc ggctctcggg
     5101 aagcgcgccg gacggaatgc catttcgccg ggtcgacggc accaacggaa tcgggagcgt
     5161 gatcctcccg cgccggacgg atccctatca gttgcctgag ctcgccaagc tgtttcccga
     5221 ttcgatcgtg cccagcgggg acggcatcta cctcctgaac gacgacctga tcgtcggaca
     5281 gggcgccacc ctcctgatcg agagccgcac cgtccatgag gttcgactcc gcagcatgcc
     5341 ggaccacttc atcacgatca cgtcctggcg cggcaccatc acggtcgcag gcaccgaata
     5401 tcagcccgtc gccatcacct catggaaccc gaacgagaac ggtcctgact taaacgaagc
     5461 agatggccgg gcctgggtgc aaacgcgccg cggatcgatg gatctgacct gggccaatac
     5521 gtcatacctc gggttcgata ccggcacgct gtccggcgtc gcctgggaag ggcggccgga
     5581 tgaacaggtc cacgggagcg cgatctactc cacgttcatg cacaaccact tcggtgccta
     5641 taccttcgag gccaccgcga tggagttccg ctacgacacc ttcgcctaca acaccgggta
     5701 tgggctcgac cctcatgacc acagcaacca cttcaccgtc gagtacaacc gcgcatacaa
     5761 caacggaacc catgggatca tcttttcgcg cggctgcgac ttcaacgtga tccggttcaa
     5821 cgcgtcctac aacaacggga cgcacggcat cgtgctggat gatgggccca acgtgaaccc
     5881 ggacggcacg atccgcgagc gtgccggtat cccctcgaac aacaacagcg tgacccagaa
     5941 caccgtctac gggaaccaaa tcgggatcgt gctggacggc gggaccggca atacgatctc
     6001 cggcaaccag atccgccaga acacctacgg tatccggatg aaggacgcgg tcgatgaaaa
     6061 cgccgtgacc ggcaaccagt tcatcgacaa tacccagttc gccatctacc tctacaacgg
     6121 ttccaatcgc aatgtcatcg ctcacaacac cgtttccggc gggaccagcg gaatcgtgat
     6181 caaggactcg gcgtcgaatc aggtcgactc caatacgctc aagggaatca gcgatcgcgg
     6241 tatcgccttc atcggcaaag ttagcgagag cgagatcacg aacaacaacg cagaccaggt
     6301 tagtacgccc ctccagctgc ccgacgtcgc cgtctccggc ccggtgacga tgcagggcaa
     6361 ttcctttgac gcctcgcgcc cctcggacac gcatccgcgc tcgattcacg ccccggccgt
     6421 acgctggctc atctgggcat tcctcctgct cttcccggtg ctctttggct cgcgcgtgct
     6481 ccgcttactc cacaacgtgc ggatgagccg gccgtttgcc atgcgaccca agtaggtcat
     6541 gccaaaagga tgtggaaacc cgtgccagtg cgagacagga cgggcagtta gcacaggacc
     6601 aatttcgact cgcggccgca ttgccaatcg agtaacctga gcgaaactgt ggctctcggg
     6661 tggagcgcac catggcgcaa cagactcgat tctggcttta ccgggcgggc accccggtgc
     6721 attcgacgct cggtgaactc attggccacg ttgtcgcggt ccggccggac gcggtgctgg
     6781 tcgctggtga ttccggacac gagcgctggt tcccggcgag tgagatcgcc gagttcagtg
     6841 atcgtggtct gatcgtctgc gtaagccagg agattgcctg gaacctaccg cccaccgcat
     6901 cacccaggcc cgacgatcgg cacgtgccgg attcgcgctt gttcttgcgc gaccccctga
     6961 atcgctacga cgcatgatcg acggtctctg accttgtcga gccgttcccc gaagcattac
     7021 ctgaaccctt gcgcgagtcc ccaccgctcc ccgggcgtac gtggcgaatc catcgtatgt
     7081 agcgtgcgca tcgttgacac gctcattagc cgggaataca ttcaagttgg tcacgcattg
     7141 caacttttga gactcatcct gcgcccgcag gtagttcccc cgtctctctg catgcgtgtc
     7201 cagcggaacg ccgcagttcc gcagggtttt ggaggagggc agaccccgtg tcccacccgc
     7261 aggctgccag ggccatccgg gcattcttcg tcgttgcgct gattggagca ttcgcggttc
     7321 atgttgaggc gccgcggccg gtttcggccg ccgccacgct ggtcagcatg tcggcctcgg
     7381 gtagcgcgac cggtggcaag cgcgtccata tccgggtcgt gatcagcgag ccggcgccgg
     7441 tcggcgggct cgatattccg ctcagctcca acaatccggc ggtgccggtc ccgtcgcacg
     7501 cgcacgtgaa ctccggcgcg accgaggagc tcgtgggggt cacgaccagg ccggtggtcg
     7561 acaccgtgat cgtcactgtg accgccagct acaagggcgt tgtgaagtcg cggcccatca
     7621 cgatcaagga gccgattcac agcagcctca gcctacagag tgtgattcgc gccggtgggc
     7681 aagggcgcgt cacggttcgg ttgagcgggc gggcgccggt tggcggcatc acagtccagt
     7741 tgtcgaccga ccggtccgac aagtttgccc tgccgacgtc ggtcacggtc ctggaaggcg
     7801 cggctagcgc gatcctgaag gtgaacgcga ccgatcaggt cccggatgtg ccgatctcgg
     7861 ttacctccca ttaccagaag gggtcgatca acaacaccta cacgaagaac gcaatcatcc
     7921 gccacttcac cgccacgccc actccaacgg cgaccaatcc gacggacgtt ccctcgatta
     7981 cgcccacgtg gacgatgacg ccgaccgcaa ccgcaacgcc ggatccgaat aacccgacct
     8041 tctccttcac ggtcaagagc ggagacccgg tagtctccgg aggatcggtc gttctcactg
     8101 tttgcatgct gacggggccg aacccgccct acacgatcac cttcatatcg acttctcagc
     8161 aacgggcaat ggtgaccgcc ggatcgccac atacatatgc cgcgctcaac gaatgtacag
     8221 atgtcacatt gactgatttg ccgtacccaa ccagtggaaa cgtacgtttg cgcgcgcggc
     8281 tctctccctc caatgtcact gttggagagt cggcgaacat caacttcccg ggcgagccgc
     8341 tgacggcaac gccgacaagc acgacagcag cggcccaaag cgccaaccag ccggcccagc
     8401 cgacgagcac tcccgtcccg acggaaccga gcgagcccat tctgctcctc tctccggaag
     8461 gaactcccta accagcagct cccctgccga atacggcagc cgagcaaata aggccaggag
     8521 agcggtctgg gttgggccgc tcttttggcg tcccgcgatt gccgggtttg gaatcctcca
     8581 cggcactgta gtcggcattg gtcgactgcg ttcgccacgt tgctaaggtt tcgactcacg
     8641 cccgtttcga cgggtcagtg aaaggacgca gccatgtcaa ctgatcacgc atatcccgaa
     8701 cgccgggacc ttgacgccca gggcaacctc ctctcagccg cgcaggaggg ccaccggcat
     8761 ccgtgggagt cccgcctcaa cggcgatgag cttgcttcga tgccgtacct cgaacccggt
     8821 acgcgacttc aggacggaca gtcctatctc gaccttaatc agctcaatgg cggcccgttc
     8881 acgccgtccg gaggtcgatc ggtccaggca ggcgaccgga tcgtggccca gcgcgacctc
     8941 gatcccgacc tctggcgcaa gctggtcggc gagggctgag ccgtctcaac ccccaacgcc
     9001 gcatcaggga ggacagcaat gtctgacgat tacagccccg gcgacgcccg caaaccctat
     9061 tccgatatcg gcggcccggg cgcgaagcac gcccagacgc acgacgtccg gcgcgagcaa
     9121 ctgacaaacc ccaagggatc ggagccggtc gacaccagct tcgccgcgca gacggagccc
     9181 ggcacaccgt gggagccggg catccacgcg gacgagagct cgcccgccgt cgacgacaag
     9241 aacctgcacg accgcctgcc gcagctcacc aacgatgagc tcgcgcgcct gccggtgctc
     9301 gatgccgaca cgcagttgga gcaaggcagc gtctacctcg atctcaatca gctcgacaag
     9361 gggccattca ggacgctcgc ccgatctgct tcgcggaacg agcggctcat cgccaagcag
     9421 cagaccgact acctgctctg gaatcgactt gccattgagg atgccgaccc gacggtcgag
     9481 cggccggaag gagccggaat gtgaccgagc ccggcgagcc accacactcg cacggtctcg
     9541 gcacgcccga aacgaccggg acgatcgaga tcgtgcgcca gttcaaggcg ccgccatcgg
     9601 cggtattccg ggcatggact gatccggcgg tgctcacgcg ctggttggcc cagaaggcgg
     9661 aatccgaccc tcgaatgggc gggcattatc gtctggagac ggatgagccc ggcgatgtgc
     9721 tggagccgca cgtctgctcc ggcgagtacc gcgagttcgt ccccgatcag cggctcgtca
     9781 tgacctggat ctacaacggg ccgcatccgg aggatctggc cgagtcgctc atctcgatcg
     9841 acttcgcgcc gatcgacgga gataccaccg agatccactt ccgcgaggtc ggcgaaggac
     9901 tagcgaccga cgaagagcgc gactattccc gcacagcctg gcaaggcgcc ttcaacaacc
     9961 tggaccgcgc gatcatcgaa tcgcacaccg tttgaccggg atctacgtcc gaagtccgaa
    10021 gcccgaagtc tgacgactca cgactgcgac aggcgcttcc gctccttggc ccggtcgctc
    10081 tcgttgagga gacgctttcg aaggcggtac gccttcggcg tgacctcaag caattcgtca
    10141 tcggccagga aatcgaggct ctgctccagg ctgagcttga ccgccggcac gagcttgatc
    10201 gcgatatcgg ccgtgctcgc ccgcatattt gtcagcttct tggcccggca gacgttgacg
    10261 accatgtcgc cctggcgcgg ctgctgcccg acgatctggc ccatatagat atcctcgccc
    10321 ggctcgacaa aggtgatccc gcgctcctgg gcgttcgcca cgccgtgcgc gaggacctgc
    10381 ccgggctcgc tcgcgacgag tgcgccggtc cggttgttcg ggatcgtccc cagccacggc
    10441 tcgtagccga tcaggcggga gcccatcacc gcattgccct tggtcgcggt cagaaatgcg
    10501 ttgcgcaggc cgatcagacc gcgcgtcgga atcgcgaact cgagccggac actgccgcgg
    10561 ccgtcgttga ccatgtccag catccgcgcc ttgcgggtcc cgaccaggtc catgatcacg
    10621 ccgaccgaat cctcgacggt ttcgatcacg aggtgctcga ccggttccat cgtcaccccg
    10681 tctatctccc gggtaatgac ctcggggcga gagacctgga actcgtaccc ctcgcgccgc
    10741 atcgtctcga tcaggacaga aagatgcagc tcgccgcgtc cggagaccgc gaagacatcg
    10801 gcctggtcgg tcggttcgac acgcagcgac agattcgtct ccaactcccg gtacagccgc
    10861 tcgcgcagct ggcgggatgt cgaataggtc gcttccttgc cggagaaggg cgaggtgttg
    10921 acgccgaacg tcaacttgag cgtcggctcc tcgatctcga tcgtcggcag cgcctcaggc
    10981 gcattcggat cggccagggt cgcgccgatc ttggcatcgg cgaatccagc cacggccaca
    11041 atatcgccgg caatcgcctc atcgacccgc cgcttgccga gtccctcgaa cgtggccaga
    11101 tcggtaatcc gctggcgggt ttcggtgccg tccggcgcga tgtggatcag cgtctcgccc
    11161 gcccggatca cgccgcgatg gacccggccg atcgcgatcc ggccgcggtg cgtgtcgtag
    11221 tcgagcgagc caaccagcat ctgcaccggc gcctcggccg cgaccttcgg ggcgggaatg
    11281 gtgtccacga tcgtgtcgag cagcgggcgg aggtcctcct ggagctgatc cggcgcaaac
    11341 ccggcccggc cctgcttggc gatcgcgtag atgatcggga aatcgagctg gtcgggatcg
    11401 gtcgcgaggt cgaggaagag atcgtgcgtc agctcgacga cctcgtcgca gcgcgcggcc
    11461 ggccggtcga tcttgttgac gactacgacc ggtgtcagcc cgagcgcgag cgcccgctgg
    11521 agtacggtcc gggtctgcgg catcggtccc tcgaccgcat cgaccagcag caggcagcca
    11581 tcggccatgt tcagcacccg ctcgacctcg ccgccgaagt cggcgtggcc gggcgtgtcg
    11641 ataatgttga tcttgagacc gcggtaggtg atcgccatgt tcttggcgag aatcgtgatc
    11701 ccccgctccc gctcgagatc gttcgagtcg gcgatcagtt ctccggccgc gtccgggtcg
    11761 cggaagatgt gcgactgctt gagcagacca tcgacgagcg tggtcttgcc gtgatcgacg
    11821 tgggcaatga tggcgacgtt gcgcaattgg gccgccgaga caggcgcgga gggcgccgca
    11881 atgggtgctt ccatgaggtg tcgtgaccga tcggttgaag cggagggtat cgaaaggcga
    11941 tgcggcccgg gcgaccctgc cccggcgcgc aacgtcccat tatatcacgc caaaggttat
    12001 ggaaactccg cagcaattgc cccgtcatgt tgggtgatac ggagttctag gcctggtctg
    12061 tctcatcctc aggcacatag tcgggccgca tccgccgttc agtttcgatc cagtcctgga
    12121 tgatagcttc ctcgaagccc cggttctcga tctcgataat tgcatcgata tccgtcgggt
    12181 gatcgggcga caccaggtgc cgaaatatcc cagcggtctt ttcaacctca cttcgcgcag
    12241 aattgatgtt caacccgccg atcctatcta ccatcattga ccagcctctt gagttggctc
    12301 acacgctcgt gatcaggcca tctggaccaa t
//
LOCUS       DATAOZ010000491         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3093274, whole genome shotgun sequence.
ACCESSION   DATAOZ010000491 DATAOZ010000000
VERSION     DATAOZ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3093274"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VHR64_12705"
     CDS             <1..624
                     /locus_tag="VHR64_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3303737.1"
                     /translation="IASDRVSGTTRLIFARPVPRSTYFLGKLAGIGAALGGILAFSYV
                     LSAVSLVIVNGSVPTAEEFGRLALFYLLSLAYMLLFALVGVTAALVVPGRSLALLVAL
                     GVWMVVTFMIPQFTSGLRPTASLNPVVNPVATTQRFFDVTSHFRAVSLSELYRDSSAA
                     VLLTAPDGSRTVGSGELVPITAFLVILVALMAALVNRLDLSEQRTHE"
     gene            617..>1515
                     /locus_tag="VHR64_12710"
     CDS             617..>1515
                     /locus_tag="VHR64_12710"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3303738.1"
                     /translation="MSSAALITLAGHDYRSAVRNRVLAALLVTLLVVTTVSVLIAAFD
                     YQTKIDDYQQYKKAAAAAGITNVAPLQVRPLQLMRPVVEYIEFVGAVIAIALGYLSVA
                     RERGNCTIWLLRTRPVSSGALTFGPFLGALGLISTLIVATAAIGVVSIGVWGHDWLNG
                     AELVRLALWCIASIVYMGIFYCLGAVLTARSRVLANGLVAALVVWLVVVLIIPQIGDT
                     MDPDNQLPGGLFKSMNLDKSHETRVLAEFSTYERLRNDLEEVSFAKQYERFSFAILEI
                     KLKDQFRDTTIRHVIGATRRNIE"
BASE COUNT          226 a          513 c          459 g          317 t
ORIGIN      
        1 atcgccagtg atcgggtgtc cggcacgaca cgcctcatct ttgcccggcc cgtcccccgc
       61 tccacctatt tcctgggcaa gctggccggc atcggggctg cgctcggcgg aatccttgcc
      121 tttagctatg tcctcagcgc agtttcgctg gtgatcgtca acggttccgt gccgaccgcg
      181 gaagagttcg gccggctcgc gctcttctac ctcctgtcgc tcgcctacat gctgctcttc
      241 gcgctggtcg gcgtaacggc ggcgctggtc gttcccggcc gatcgctggc gctcctcgtc
      301 gcgcttggcg tctggatggt cgtcacattc atgattccgc aattcacctc gggcctgcgg
      361 ccgacagcgt cgctgaaccc ggtcgtcaac cccgtcgcca ccacgcagcg attcttcgac
      421 gtgacatccc acttccgcgc ggtgtccctg agtgagctct atcgcgattc aagcgccgcc
      481 gtcctgctca ccgcgccgga tggttccagg acggttggct cgggcgaact cgtgcccatc
      541 accgcatttc tggtcatcct cgtggcgctg atggcggcgc tcgtcaatcg gctcgatctc
      601 tcggagcagc gtacccatga gtagcgcggc actcattacc ctcgccggtc atgattaccg
      661 gtccgccgtg cgcaaccggg tcctggcggc gttgctcgtg acgctgctcg tcgtgaccac
      721 ggtttcggtg ctgatcgcgg cgttcgatta tcagaccaaa atcgacgact accagcaata
      781 taagaaggca gcggcagcgg ccggcatcac gaacgtggcg ccgttgcagg tcagaccact
      841 ccagttgatg cgaccggtgg tcgaatatat cgagttcgtc ggcgccgtga tcgccatcgc
      901 gctcggctac ctgagtgttg cgcgggagcg cgggaattgc acgatctggt tgctgcgcac
      961 ccgcccggtc tcctcgggcg cgctcacgtt cggaccgttc ctcggcgcgc tcggcctgat
     1021 ctccaccctg attgtcgcga ccgcggcgat cggtgtcgtg tcgatcggtg tctgggggca
     1081 cgactggctc aacggggcgg agctggtccg gctcgccctc tggtgcatcg cctcgatcgt
     1141 ctacatgggc atcttctact gcctgggcgc ggtgctcacc gcccgcagcc gggtcctcgc
     1201 caacggactc gtcgcggcgc ttgtcgtctg gctggttgtg gtcctgatca tcccgcagat
     1261 cggcgacacg atggacccgg acaaccagtt gccgggcggc ctgttcaagt ccatgaatct
     1321 cgacaagagc catgagaccc gggtgctcgc cgagttcagt acctacgagc gcctccgcaa
     1381 cgacctcgaa gaggtctcgt ttgccaagca gtacgagcgc ttctcgtttg cgattctcga
     1441 aatcaagctc aaggaccagt tccgcgacac gaccatcagg cacgtcatcg gcgcaacccg
     1501 gaggaacatc gagtg
//
LOCUS       DATAOZ010000492         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6774647, whole genome shotgun sequence.
ACCESSION   DATAOZ010000492 DATAOZ010000000
VERSION     DATAOZ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6774647"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VHR64_12715"
     CDS             <1..506
                     /locus_tag="VHR64_12715"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303739.1"
                     /translation="ALTEARMDGAVKTSGAKGVHVFVPLATGSSPEDVAAATRAVAVR
                     AERIDPDLATTAFVKDERGGKVFLDSTRAGGATVVSAYSPRIRPGVPVSFPVAWDDLD
                     QIAPSDFTISNAAGLLRDADPWADRMPPAQQLDADLVAEGHTVPVARVQAMHEGKRRA
                     RSQRNTS"
     gene            661..>1584
                     /locus_tag="VHR64_12720"
     CDS             661..>1584
                     /locus_tag="VHR64_12720"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX3303740.1"
                     /translation="MIEIDVILLVGAVVLIAAIIAARIGARVGLPSLLLFLGLGMIMG
                     ESVIGISFDNADLAQALGFAALVVILAEGGLTTKWSDIRSSTILAALLATVAIGISVG
                     LMTLFGHYVLGLPLWIAMLLGAVTSPTDAAAVFSVLRNVPIPHALRGTLEAESGLNDA
                     PTVLLVMVASSLAIGEPLEFGVAGLAALIVLELVGGLIIGGGLGWLGAQILRRVALPS
                     SGLYPLAILVWTVFAYGITALIHASGFAAVYVCALILGNVQLPHRAATRSFVEGIGWI
                     AQIGLFVMLGLLAFPDRVTPRAAVIAVLAGLF"
BASE COUNT          226 a          523 c          504 g          331 t
ORIGIN      
        1 aagcactgac cgaggcacgc atggacggtg ccgtgaagac gagcggcgcc aagggcgtac
       61 atgtgttcgt tccccttgca accgggtcat ccccggaaga tgtggccgcg gcgacgcgcg
      121 ccgtcgccgt acgcgccgag cggatcgatc ccgatctggc gaccaccgcg tttgtgaagg
      181 acgagcgcgg aggcaaggtc tttctcgact cgacccgcgc gggcggcgcc acggtcgtgt
      241 cggcctacag cccgcgcatc cggcccggcg taccggtctc attcccggtc gcctgggatg
      301 accttgacca aatcgcgccg tccgatttca ccatctccaa cgcggccggt ctgttgcgtg
      361 acgctgatcc ctgggccgac cggatgccgc cggcgcaaca gctggacgct gatcttgtag
      421 ccgaaggtca cacggttcct gtcgcccgag tccaggcgat gcatgaggga aaacggcgag
      481 ctcgctcgca acgcaacacg agctagattg ctccgctcgc ttcgctcgct cgatccgacc
      541 gcctgaagcg ctctctgctc cgtttctccg cacaaggcca catatgaccg ccgaatcggc
      601 aatttttgct tgcgtacgcg gctttgtgcg gagaaacgca acgtggggaa ggatgtgcgc
      661 atgattgaga ttgacgtcat cctgctggtg ggtgccgtcg tcttgatcgc agcgatcatc
      721 gccgcccgga tcggcgcccg cgtcggactc ccatcgctcc tgttgttcct cggtctcggc
      781 atgatcatgg gcgaatccgt catcggaatc tccttcgaca atgccgatct tgctcaggcg
      841 ctcggcttcg cggccctggt ggtgatcctg gccgagggtg gtctcaccac gaagtggagc
      901 gacatcaggt cctcgaccat cttggccgcg ttgctggcca cggtcgcgat cggaatcagc
      961 gtcggcctga tgactctctt cgggcactac gtcctcggac tgccgctgtg gattgcgatg
     1021 ctgctgggtg cggtgacgtc cccgacagac gccgctgcgg tcttctcggt gctgcgcaac
     1081 gtccctattc cgcacgcctt acgcgggacg ctggaggccg aatccggcct gaatgatgcg
     1141 ccaacggtgc tgctcgtgat ggtggccagt tcgctagcga ttggcgaacc gctggagttc
     1201 ggcgttgcag gtctggccgc cctgattgtg ctggagctcg ttggcggctt gatcatcggt
     1261 ggtggccttg gctggctcgg tgctcagatc ttgcgccgcg ttgcgctgcc gtcgtccggg
     1321 ctctacccgc ttgcgatctt ggtctggacg gtgttcgcgt acggcatcac cgcgctcatc
     1381 cacgccagcg ggttcgccgc ggtgtacgtc tgcgcgttga tcttaggcaa cgtccagctg
     1441 ccacaccgcg ccgcgacccg ttccttcgtc gaagggatcg gctggatcgc ccagatcggg
     1501 ctcttcgtca tgctcggctt actcgccttc ccagaccggg tcactccacg cgccgccgtg
     1561 atcgccgttc tggccgggct cttc
//
LOCUS       DATAOZ010000493         4745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_984512, whole genome shotgun sequence.
ACCESSION   DATAOZ010000493 DATAOZ010000000
VERSION     DATAOZ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984512"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2559)
                     /locus_tag="VHR64_12725"
     CDS             complement(<1..2559)
                     /locus_tag="VHR64_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303741.1"
                     /translation="MKHLALLLTLIASMVSWSPPLSRAADVSSLTLSSTSVVGGKTST
                     ATVRLDSAALGGDLVIALSSSNPSAATVPGSVTIPGGSSSATFTVTTVPVSASTAVVI
                     TASGPGISKSANLTVTPPALSAVSASPLTIVNGRTGTGTVTLTGSAPAGGIVVALSSS
                     SSLLDLPAQVTVPAGSKNVTFEMTAGIAPSTTAVTVSATLGVTTKSTTVNVAPLQVAS
                     VSFTPASPSGGTVVKLKVTLNGAADGSGAVVALSGGDPSLLPLPGTITIPPGTTNATI
                     SVATGFVSTATPFTVRAGLNGASRSATITLNPVAINAISASPSSITGSLTATGTATLN
                     GPAPAGGVVVALASSNTAAFRVPSSVTVPGGATSATFSITTSYVTSTTSVTLSASFDG
                     ATKSATAKVVPVTVAGVSMLPASIVGGQSPSIGTVKLTAAAPSGGVVISLTSSDRVVT
                     VPASIMIPAGQLSGTFTANSTAVTASTISTIRATDGRTTRTAKVTVNPLGLASLGISG
                     TQYSGWTANAVISLNGVAPAGGAQVLLSVSDPTKASAPGSLLIPQGESKLTFTIDFSL
                     VSAETPVVITATYAGVAISVPVTIKPATISKVTLSPGSVTGGNTSTGTVWLTGKAPSG
                     GLVVTLTSSSATALVSSSISIAQGGETGTFTIRTTAVSATTAATISATANGVKQSATL
                     SIAPPKIATISPDPIFVSAGGSAPVVLSLDGVAPANGIRVALSSNNVAVATVPASILV
                     PAGASSVTFQVTGSSGAVQYQTANITANYGGQSLTALVGIDSLRVTALGFNPGIIVSG
                     QSSTGTVYLSGTAPAGGLVVTLSCYTPSLTMPATIAIPAGSSSATFIVTASASAP"
     gene            complement(2915..>4745)
                     /locus_tag="VHR64_12730"
     CDS             complement(2915..>4745)
                     /locus_tag="VHR64_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303742.1"
                     /translation="SSSGEATATFENERSNGTAAITIHKATCPETTKDLFKECHDDRL
                     SDINFTIGSERVKTDKKGVATANIASGTVRITEAEADFTQNATVGAAYVYCSVQPGST
                     DVLYDAVANHRSVDIKVKDGTTVVCDWYDLTSKGAAQNGILELHKRVCPNGPPTGDIF
                     QTCHDNLPQQPVAFSVDSGAAKFVGENGNVTFDGLTAGTHAVQETEGPPLEFVELRIW
                     CSVQGSDAAPFQVQPNGPNFSVPVGDGQHVICDVYNIAINLSGLTPTPPPAQTPTVPS
                     VETPTTVPAQATNTPTIAPPPPPTETPTPVPPTATATMTETPVPPTPTPTPVGRPIQV
                     QAGTCNDLSDQPGFALNSLTTPEGTEQGSDRSTVAEASYTAINISLNELLTSDHSITV
                     RKSHASNRTIVACGEIGGPTRSDGSIVVGLRELGNSGLTGVAYLLADPNNANRTRVSV
                     FLAPGLAEEDPTLKPASAEALVVDSQVDNGPVAPESQEGYEIKIFGSGRAEITITPPG
                     ASDALGSQTTAEKQMRTVDLTDAEFTQLLQQLQDAGYFRLAQANDINPNSQTVGGSTS
                     LLNVSLVDGDWTVNGNGLTDSQANTLEKAQKIVSDAVGGVELP"
BASE COUNT          814 a         1442 c         1613 g          876 t
ORIGIN      
        1 cggcgccgag gcgcttgcgg ttacgatgaa ggtcgcgctc gaagaaccgg ccggaatcgc
       61 aatggtagcc ggcatcgtca aggatggggt gtagcacgac agggtgacca caagaccgcc
      121 agccggcgcc gtgccgctga ggtagacagt tccggtcgac gattgtccgc ttacgatgat
      181 gcccggattg aacccgagag cagtcacccg caacgagtcg atccccacga gtgcggtgag
      241 cgattgccca ccgtagttgg cggtgatgtt ggctgtctga tattggacgg cgccggatga
      301 accggttacc tggaacgtga cggatgatgc gccggccggg accaatatcg acgcgggcac
      361 ggtggccacg gccacgttgt tgctggacag ggcgacgcgg atgccgtttg ccggcgcaac
      421 tccatcgagc gacaggacga ctggagcgct gccaccggct gagacaaaga tgggatcggg
      481 cgaaatggtc gcgatcttag gcggggcaat cgagagggtc gcgctctgct tcacgccgtt
      541 ggcggttgcc gagatcgtgg cggccgtcgt cgccgaaacg gcggtcgttc gaatcgtgaa
      601 tgtcccggtc tcaccgccct gggcgatact aatcgacgaa gacaccaatg cggtcgcact
      661 cgagctggtc agtgtgacca ccagaccacc agacggcgcc ttgccagtca accagaccgt
      721 gcccgtggat gtattgccac cagtgacgga gcctggcgag agggtcacct ttgagatggt
      781 cgcgggcttg atcgtaactg gcaccgagat cgcgacgccg gcataggtcg ccgtaatgac
      841 gaccggggtc tcggcgctga cgaggctgaa atcgatcgta aaggtgagct tcgactctcc
      901 ctgggggatg agcagcgatc ccggcgcact tgccttggtc ggatccgaaa cggagagaag
      961 aacctgagcg ccgccagccg gggcgacgcc attgagactg atcacggcgt ttgccgtcca
     1021 tccgctgtat tgcgtgcccg agataccgag cgacgcgaga cccagaggat tgaccgtcac
     1081 ctttgctgtt cgcgtcgtcc gaccgtcggt tgccctgatc gtggatatcg tcgacgcggt
     1141 cacagcggtc gagttggcgg taaacgtgcc cgaaagctga ccggccggaa tcatgatcga
     1201 tgccgggacc gtcacaaccc gatccgagct cgtcagcgag atgacgaccc cgccagacgg
     1261 cgccgccgcc gtcagtttca ccgtgccgat ggatggcgat tgaccaccga ctatcgatgc
     1321 gggcagcatc gatacgccgg cgaccgtgac cggtacaacc ttggcggttg cggacttggt
     1381 cgcgccgtcg aatgatgcgg acagcgtcac actcgtggtg cttgtaacgt agctggtcgt
     1441 gatcgagaag gtcgcgctcg tcgcgccacc gggtacggtc acggacgacg gcacgcgaaa
     1501 cgcggcggta ttgcttgacg cgagggcgac aaccacgcct ccggccggcg ccggtccgtt
     1561 caaggtcgcc gttccggtcg cagtcagact gccggtgatc gaggacggac tggccgagat
     1621 tgcgttgatg gcaaccggat tgagcgtgat cgtcgctgag cgagaggcgc cgttcaggcc
     1681 agcgcggacg gtgaacggcg tggccgtgct gacgaagccg gtcgcgacgc ttatcgtcgc
     1741 attggtggtt ccgggtggaa tcgtgatcgt gcctggcagc ggcaagagcg atgggtctcc
     1801 tccggatagc gccacgacgg cgcccgagcc gtcggccgcg ccattgagcg tcaccttcaa
     1861 cttgacaacc gtgccgccgg agggcgatgc gggagtaaat gacaccgaag cgacctgtaa
     1921 cggagcgacg ttgactgttg tgctcttggt cgtgaccccg agtgtcgctg acaccgtgac
     1981 tgcggtcgta cttggcgcaa ttccggcggt catctcgaat gtcacattct tcgagccggc
     2041 aggcaccgtg acctgcgccg ggagatccag caatgacgac gagctggaca gcgccacaac
     2101 aatgccaccg gcgggcgcag agccggtcag ggtgacggtc ccggttcccg ttcggccatt
     2161 gacgattgtc aatggactgg ccgagaccgc cgagagcgct ggaggagtta ccgtcagatt
     2221 cgcggatttg cttatcccgg gcccggacgc cgtaataacc accgccgtgc tggcgctgac
     2281 aggaaccgtt gtaacggtga atgtcgcgct cgaggagccg cccgggatcg tcacactgcc
     2341 cggaacggtt gcggccgacg gattgctgga cgagagtgca ataaccaggt cgccgccgag
     2401 cgcggcgctg tcgagacgca ccgtcgctgt ggacgttttc ccgcccacga ccgatgtcga
     2461 cgagagcgtg agcgaagaga catcggccgc acgtgatagg ggcggcgacc atgagaccat
     2521 cgaagcgatg agggtgagga gaagcgcgag gtgcttcatc tgttcggttc cctgggtttc
     2581 tccgaaaatc ggcaacggta cctgtccgtt cggcactcgc cgccgggcag acacatcgtg
     2641 cgaccaggga gcccgctggg gcatagcccg gcagatagga agggcttcgg taccgaagtc
     2701 ggctcccggg caatacggat ttccgattga atcttgatcc gatatgaata gatcggaacg
     2761 cgatcaagcc caacacgcat cccggcgtga ctcggggtgt cggaaatgcg ccctcaatcg
     2821 atttggagtg gggagctgct cgggtggtcc gaaaatgcca ggcggggata ggccaggcct
     2881 atccccgtac gatcgtcaaa ctggttgtgt cgcgctacgg cagctcgacg ccgccgaccg
     2941 cgtcggagac gatcttctgc gccttctcga gcgtgttcgc ctggctatcg gtgagaccgt
     3001 tcccgttcac ggtccagtcg ccgtcgacca gcgagacgtt gagcaggctg gtcgatccgc
     3061 ccacagtttg gctgttgggg ttgatgtcat tcgcctgcgc cagcctgaag tacccggcgt
     3121 cctgaagctg ctggagcagt tgcgtaaact cggcatcggt caggtcaacc gttcgcatct
     3181 gcttctcggc tgtcgtctga ctcccgaggg catccgacgc gccgggaggc gtgatcgtga
     3241 tctcggccct cccggagccg aaaatcttga tctcgtagcc ctcctgcgac tccggcgcga
     3301 ccggtccgtt gtcgacctgg ctatcgacga ccaaggcctc ggccgacgcc ggcttgagcg
     3361 tcggatcctc ctcggccaga cccggcgcga ggaagacgga gacgcgagtc cggttcgcgt
     3421 tgttcgggtc ggcgagcagg taggcgacgc ccgtcagccc ggagttgccc agctcccgca
     3481 atccgaccac gattgacccg tcggatcgcg tcggaccgcc gatctcgccg caggcgacga
     3541 tcgtgcgatt cgatgcgtgg ctcttgcgga ccgtgatcga atggtcgctc gtgagcagct
     3601 cgttcagcga gatgttgatc gccgtgtacg aggcctcagc caccgtcgac ctgtccgatc
     3661 cctgctcggt tccttccggc gtcgtcaggc tgttcaacgc aaagcccggc tgatccgaca
     3721 ggtcgttgca ggttcccgcc tggacctgaa tcggccgccc gaccggagtc ggggtgggcg
     3781 tcggcggaac cggcgtctct gtcatggtcg cggtcgccgt gggcggcacg ggggtcggcg
     3841 tctcggtagg cggaggaggc ggcgcgatcg tcggcgtgtt cgtcgcctgc gcgggaacgg
     3901 ttgtcggcgt ctcgaccgac ggaacggtcg gcgtctgggc cggtggcgga gtcggagtca
     3961 ggccgctcag gttgatcgcg atgttgtaga cgtcgcaaat gacatgctgc ccgtctccga
     4021 ccggcaccga gaagttcggg ccgtttggtt ggacctggaa cggggccgca tcgcttccct
     4081 ggaccgagca ccagatccgc agctcgacga actccagtgg cggcccctcg gtctcctgga
     4141 ccgcgtgcgt tccggcggtc aggccatcga acgtgacgtt gccgttctcg ccgacgaact
     4201 tcgccgcgcc ggaatcgacc gagaaggcga cgggctgctg cggcaggttg tcatggcagg
     4261 tctggaagat gtcgcccgtc ggcggaccgt tcgggcatac gcgcttgtgc agctcgagga
     4321 tgccgttctg cgccgcgccc ttgctcgtca ggtcgtacca gtcgcagacg accgtcgtgc
     4381 cgtccttgac cttgatgtcg acgctgcgat ggttcgccac ggcgtcatag aggacatcag
     4441 tgctgcccgg ctggaccgaa cagtagacat aagcggctcc tacggtcgca ttctgggtga
     4501 agtcagcctc ggcctccgtg atccggaccg tgccggacgc gatattggcc gtcgccacgc
     4561 ccttcttgtc cgtcttgacc cgttcgctgc cgatcgtgaa gttgatatcg gagaggcggt
     4621 cgtcgtggca ttccttgaag aggtccttgg tcgtctccgg gcaggttgcc ttgtggattg
     4681 tgatcgcggc ggttccattg gaccgctcgt tctcgaaggt cgcggtggcc tcaccgctcg
     4741 agctc
//
LOCUS       DATAOZ010000494         4012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1780702, whole genome shotgun sequence.
ACCESSION   DATAOZ010000494 DATAOZ010000000
VERSION     DATAOZ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780702"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(465..932)
                     /locus_tag="VHR64_12735"
     CDS             complement(465..932)
                     /locus_tag="VHR64_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX3303743.1"
                     /translation="MNGVEVLLRGPDHWNHASPTDWAEVLATFPLFSAISKRRLRKLV
                     RNATVAEHGPGHIVIQKGTSGDSLYLILGGSAKALGKPASRPLRTGDYFGELSLIDGA
                     PRSATVIATSELHLMTLPRHTFLELMHNAEISLQMLRTLGAQVRRLEARPAQA"
     gene            complement(929..1177)
                     /locus_tag="VHR64_12740"
     CDS             complement(929..1177)
                     /locus_tag="VHR64_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303744.1"
                     /translation="MSVAIPIPIPIPIPIPIPRQELAQRTSGGLEITLYWDAEKDGTI
                     VEVHQPATQETIAIPVTHDQALDAFHHPFAYLARTRSS"
     gene            complement(1256..2254)
                     /locus_tag="VHR64_12745"
     CDS             complement(1256..2254)
                     /locus_tag="VHR64_12745"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303745.1"
                     /translation="MQHFPFGTATALSHFVRLLVGAAMLTVVTIAAASAFAAGGSAVV
                     KVGPSNLGRVLVDAHGKTLYIWAHDKSSKSTCNGDCAEYWPPLVTKGRPQAIAGARSD
                     LLGTSRRTDGRIQVTYAGHPLYYFVKDTKAGQTNGEALTGFGGRWDPISASGQAVRKG
                     GTGTSGSSGGYGDEAAPLQASIISPGPGDTAGVHGTFSVEVSLQARTAYSNGLLSGYT
                     AAFNDPSKPTFHPGPNAAAPGLVVLLSTTPTLAGTPLKGPNTNLAGVFQINDVSQVKG
                     LTRTFNSWIVTSAGFFGKNVKATLTAYAVNGTAPALITGTEKPISNVVRETFTIAA"
     gene            2345..2977
                     /locus_tag="VHR64_12750"
     CDS             2345..2977
                     /locus_tag="VHR64_12750"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3303746.1"
                     /translation="MTDTKPTPNRRSERSRQAILGATRELLAEDGGVRALTVEAVAAR
                     AGVGKTTIYRRWRDKWELALDAVMIDMLPRFANPIDVSDTRKELITFVDSVTKLWASP
                     PYGPVMQGLISQIATEPELARVYRDQVVEPRREQLRPVIERGIARGDLRPDADVRLVH
                     ELLVGPILYRLLLSGPPLDRKLTTSLADAVLDSFKPRTAEGPRRKRPSRR"
     gene            complement(3300..3833)
                     /locus_tag="VHR64_12755"
     CDS             complement(3300..3833)
                     /locus_tag="VHR64_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303747.1"
                     /translation="MSTTTIENQLTEDRRRIARLQAFAQSGLVADRARIQRHLDALHK
                     EEAAALAALRRGPDEVVEKLGQLRTRIAVAENSLVADVSGEWMTFAAAVEDELRSWDT
                     YLERLQATAVAKAGNARDQAEAAIAGVRTQRIAVHDCLAQAPVNVNGAWHEQRKHVSW
                     VRDELEQKADELSARIK"
BASE COUNT          611 a         1276 c         1356 g          769 t
ORIGIN      
        1 cggtccgcgg tagagactga caaaccaccg aaaacctcat cgctgcttcg gccgatccag
       61 aggccacttc ttcgggcgtc atcgccgaac actggcccag ccaggcccgg ccgttcgact
      121 gccatcgcct ttatgtagtt gtgcgcgacc ttccttagaa caccgccacc actaccgggt
      181 gagatcaagc gcgaagagcc ggtgcacgcc ttcggtcctt tcagttccac ctcgcgactc
      241 tcttggaagt cgattccagg gccggagaca aggtcgtgca ctacgttgga aaccaggact
      301 tcgccgccga gggcatggct ggcgacgcgc gccgcatagt gaaccgtctt gccgaagaag
      361 tggtcggctt cctgtagcgc gtcacctgtg tggactccga ttcggacgcg gatcggaggc
      421 gagaatttgg cttcggccgc gcgccgcgcc ccgggtcacc ggacctatgc ctgcgctggc
      481 cgggcctcca gccgacggac ttgcgcgccg agtgtacgaa gcatctgcag tgagatctcg
      541 gcgttgtgca taagctcgag gaaggtgtgg cgtgggagcg tcattaggtg gagttcgctg
      601 gttgcgataa cggttgccga tcggggagcg ccgtcgatga ggctcagttc tccgaagtag
      661 tcgccggtgc gcagcgggcg tgaggctggc ttgccgagcg cctttgccga gccaccgagg
      721 atcaggtaaa gcgagtctcc ggatgttccc ttctggatga cgatatggcc gggtccgtgc
      781 tcggcgactg tggcgtttcg gacgagcttt cgcagtcgcc gcttgctgat cgccgagaac
      841 aatggaaagg ttgctagcac ttcggcccaa tcggtcggac tcgcgtgatt ccagtgatcg
      901 gggccacgca gtagtacctc tacgccgttc atgatgacct cgttcgcgcc aggtaggcaa
      961 aggggtggtg gaaggcgtcg agtgcctggt cgtgggtgac tgggatcgcg atcgtctcct
     1021 gggttgctgg ttggtgcact tcgacgatgg tgccgtcctt ttcggcatcc cagtagagag
     1081 tgatctcgag gccgccgctg gtgcgctgtg caagttcttg gcgggggata gggataggga
     1141 tcgggatagg gatcgggatt gggatggcga cgctcatgct tatttcattc ctttctcttg
     1201 tggcgggtgc ggcagggcgc cgacggtgtc gcgccctgcc gctgtctggt ttcgcttagg
     1261 ccgcgatcgt gaaggtctcg cggacgacgt tggagatcgg cttctcggtg ccggtgatca
     1321 gggcgggagc ggtgccgttg accgcgtagg cggtcagcgt cgccttgacg ttcttgccga
     1381 agaagccggc gctggtgacg atccagctgt tgaaggtgcg ggtcaggccc ttgacctgag
     1441 agacgtcgtt gatctggaag acgccggcca ggttggtatt cgggcccttc agcggcgtgc
     1501 ctgcgagcgt cggcgtggtc gagagcagga cgaccaatcc gggcgcggcc gcgttcggcc
     1561 cggggtggaa ggtcggcttg ctcgggtcgt tgaaggccgc cgtgtagccg gacagcagtc
     1621 cgttcgagta tgccgtccgg gcctgcagtg agacctcgac gctgaaggtc ccgtggacgc
     1681 cggccgtgtc gccgggtcca ggggagatga tgctggcctg gagcggggct gcctcatcgc
     1741 cgtagccacc acttgaaccg gacgtcccgg tgccgccttt gcggaccgcc tgtccgcttg
     1801 ccgagatggg atcccagcgg ccgccgaagc cggtcagggc ttcgccgttg gtctggccgg
     1861 ctttcgtgtc cttgacgaag tagtagagcg ggtggccggc gtaggtgacc tggatccggc
     1921 cgtcggtgcg cctgctcgtg ccgagcagat cgcttcttgc gccggcgatc gcctgcggcc
     1981 ggcccttagt gacaagcggc ggccagtact cggcgcagtc gccgttgcag gtgctcttcg
     2041 agctcttgtc gtgcgcccag atgtagagcg tcttgccgtg ggcatcgacg aggacgcggc
     2101 cgaggttgct cggcccgacc ttgacgaccg cgctgccgcc ggccgcgaac gccgaggcgg
     2161 ctgcgattgt cactaccgtg agcatggccg cgccgacgag cagccgcacg aagtgcgaca
     2221 gcgctgtggc cgtgccgaac gggaagtgct gcattgcaat actccttcct tctgatttac
     2281 gctacgagac gtagcgtcct gatcaattcg aaacgctacg tctcgttcta taattcccat
     2341 tgacgtgacc gacaccaaac cgacaccaaa ccgacgcagt gaacgctcgc ggcaggcgat
     2401 cctcggcgct acccgtgagc tgctcgccga agacggggga gtccgggcgc tgacagtcga
     2461 ggcggtcgcc gcccgcgctg gggtcgggaa gacgacgatc tacaggcgct ggcgcgacaa
     2521 atgggaactc gcgctcgacg cggtgatgat cgacatgctc ccgcgcttcg cgaaccccat
     2581 cgacgtcagc gacacgcgca aggagctgat cacgttcgtc gactccgtga ccaagctctg
     2641 ggcatcgccg ccctacggac ccgtcatgca aggcctgata tcgcagatcg cgaccgagcc
     2701 ggagctcgcc cgcgtgtacc gcgatcaggt cgtcgagccg cgacgcgagc aactgcggcc
     2761 cgtcatcgag cgtggaatcg ctcgcggcga cctacgtccc gacgccgacg tccggctcgt
     2821 gcacgagctg ctggtcggac cgatcctcta cagactgctg ctgagcggcc cgccgctcga
     2881 ccgcaaacta accaccagcc tcgccgacgc ggtcctcgac agcttcaagc cgcgcaccgc
     2941 cgaaggtccc cgacggaaac ggccgtcacg ccgctgacgc ggccgatccc ttgttcaagc
     3001 aaagcctcaa ctcatctctt gagcgggcga gttgccgcac cgtgtctacc tggagacacg
     3061 tgatctggcg agaaatcgcc gagcccaacc gggttccgcc tgaccttcac ccgataggca
     3121 gcgcatctgg gcttgtcgcg gcccgccaga ctgatgcgac gggcgaggtc tgtctggttt
     3181 cgagtctctt ggtggctttt cggtgtcgtg cgagcagtcg tgcgacacgc tgccgcaagc
     3241 gagctcgcgg aggctcaacc gcggctcgag cgcttcgaga ttcatgtcct taggccgttt
     3301 cacttgatcc tcgccgacag ttcgtcggcc ttctgttcga gctcgtcgcg aacccagctg
     3361 acgtgtttcc tctgctcgtg ccaggcgccg ttgacgttca cgggtgcctg cgcaaggcag
     3421 tcgtggactg cgatccgttg ggtgcggacg ccggcaatcg ccgcctcggc ctggtcacgc
     3481 gcattcccgg ccttcgccac ggccgtcgcc tgcagccgct cgaggtaggt atcccagctg
     3541 cggagctcgt cctcgacggc ggccgcgaac gtcatccact cgccggagac gtctgcaacc
     3601 agcgaattct cggccaccgc gatccttgtc cttagctgcc cgagcttctc cacgacctcg
     3661 tctgggcccc ggcgcaacgc ggcaagggcg gctgcctcct ccttgtggag tgcgtcgagg
     3721 tggcgctgga tccgcgctct gtccgcgacg aggccgctct gcgcgaacgc ctgcagccgg
     3781 gcgatccggc ggcggtcctc cgtgagctgg ttctcgatcg tggtcgtgct catgttcgag
     3841 gctcctgttg gtctcattct ctgacgccgc aagggtcgcc agcgccgatg ccaacgctct
     3901 gccaggacgc ggcagcgctc tgccagcttc gctaggtgag cgcggtgccg gggcgcggct
     3961 cggcgatctc ggcgagcccg cgctctgctg cagtggccgg gttgccggcg ag
//
LOCUS       DATAOZ010000495         3736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8616433, whole genome shotgun sequence.
ACCESSION   DATAOZ010000495 DATAOZ010000000
VERSION     DATAOZ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8616433"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..569
                     /locus_tag="VHR64_12760"
     CDS             210..569
                     /locus_tag="VHR64_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX3303748.1"
                     /translation="MSEATISTAVRQISPTISVIDIGGSVTRAADQALTNAYHEASGD
                     KTRAIILNFANLEYMNSSGIGLLVTLLVRANRQNQQLYACGLTDHYRDIFSLTRLDEA
                     IHIEADEAAAIATAENR"
     gene            597..1373
                     /locus_tag="VHR64_12765"
     CDS             597..1373
                     /locus_tag="VHR64_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303749.1"
                     /translation="MSTTPTDTKSTGSAADQPEPAKRSDRDSWAKPVSQLSVSGIPDD
                     AINLNVEGRRVAGPAQGFGRMWRKRHHVRLDGLDVAPQEVISTWKSNFGSFWPSGNRF
                     YGPITGLLPGDVAVINLAMPGKQKLSTGVLVLYADDESFSLMTPQGHVFAGWITFSSY
                     TEDSTPIVQAEILMRASDPIFEIGMEVMGHKRENAFWEQTLRNVASHFGIQGKAITEV
                     ECVDPHRQWRYVTNVWQNSQIRTALYMPTIPFRAAARRFR"
     gene            1633..2385
                     /locus_tag="VHR64_12770"
     CDS             1633..2385
                     /locus_tag="VHR64_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303750.1"
                     /translation="MQHESESPITPRNSGKPDTGKPDAEVFEEADALDLHQQRPTGPE
                     DGFGKLWRKLHRIDLTGADLPPEHVIATWKQHFGEFWPGQNRFYGPITALQPGELAVI
                     NIGAPPGTLSTGVRLAESTPTSFTLVTTKTHMLCGWLRFSADRSSGATTVSVEMLIRA
                     SDPIFEIGMIFGGHRWENHFWRQMLRNLAAHFGIAAEPESRVTCEDPHYRWENAKNIW
                     HNGAIRNGLVRVAALPGKASDLLRHRRTERTS"
     gene            2382..>3736
                     /locus_tag="VHR64_12775"
     CDS             2382..>3736
                     /locus_tag="VHR64_12775"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3303751.1"
                     /translation="MTSTHDAVIIGAGPNGLAAGITLAQAGRSVLILEARETVGGGCR
                     TLELTLPGYWHDPCSAIHPLAVVSPFFRSLPLADYGLEMIEPPLALANPFDDGTAAVL
                     AKSVDETAASLGPDGDAYKRLLDPLLEDQEILIPELLGPLRIPPRHPIAMARFGLVGL
                     RSAKGLAFSRFKGRNARALFAGMAGHSMLKLDQLVTGAFGLTLALFGHVANWPVPRGG
                     SQAIVDALAAHFRSLGGEIRTNTPADSISQYKDTHAILFDTTPRQFLTLAGDQVPGRY
                     RRHLQHYRYGPGVFKVDWALSGPVPWTALECRRAGTVHLGGSFEEIAASEADACAGRH
                     SERPYVIVAQQSLFDSTRAPDGNHTLWAYCHVPSGSDKDMTAAIEAQIERFAPGFRDL
                     VIARSVMGPGVIEAHNPNYIGGDINGGVQDIRQLFTRPAVRRDPYSTPNPRLYLCSSS
                     TP"
BASE COUNT          678 a         1398 c         1051 g          609 t
ORIGIN      
        1 gccggccacg cccaacatcg atgccaagct ggccggcctg caatcgccgc gcggctgggg
       61 cctgttcctg atcgagaaga tggtcgatca ggtccaagtg ctcgacacgc agaacggcca
      121 caccgtcgag ttgaccgtca acctgaatga atcgcccggc gccgattgag cgttcagatc
      181 aataaccact gggaagggga ggaaacgaca tgtccgaagc aaccatctcg accgccgttc
      241 gccagatcag cccgaccatc agcgtgatcg acatcggcgg ttcggtcacc cgtgcggccg
      301 atcaggcact gaccaacgcc taccacgagg ccagcggcga caagacccgc gcgatcatcc
      361 tcaacttcgc caacctcgag tacatgaaca gtagcggcat cggcctgctc gtcaccctgc
      421 tggtccgcgc caatcgccag aaccagcagc tctacgcctg tggcctgacc gaccattatc
      481 gcgatatctt ttcacttacc cgtctcgacg aggccatcca tatcgaagcc gacgaggcag
      541 cggcaatcgc tactgccgag aatcgctaac ctgcaacacc cggagtcacc taaccgatga
      601 gcaccacgcc gaccgacacc aagtccacgg gctccgcggc tgatcagccg gagccggcca
      661 aacgcagcga ccgtgatagc tgggccaagc cggtttcgca gctcagcgtc tccggcatac
      721 cagacgatgc catcaatctc aatgtcgaag gtcgccgcgt cgccggtccc gcccagggct
      781 tcggccggat gtggcgcaag cggcatcacg tccgtctcga cggtctcgat gtcgcgccgc
      841 aggaggtgat ctcgacctgg aagagcaact tcggctcatt ctggccgagc ggcaaccgct
      901 tctacggtcc gatcaccggt ctcctcccgg gcgatgtcgc cgtgatcaat ttggccatgc
      961 ccggcaagca gaaactctcg accggcgtcc tcgtcctcta tgccgacgac gaatccttct
     1021 cgctgatgac gccgcagggc cacgtcttcg ccggctggat caccttctcc tcgtacacgg
     1081 aagacagcac gccgatcgtc caggccgaga tcctgatgcg tgccagcgac ccgatcttcg
     1141 agatcggaat ggaggtcatg ggccacaagc gcgaaaacgc tttctgggag cagactctgc
     1201 gcaacgtcgc ctcccacttc ggcatccagg gcaaggcaat cactgaggtc gaatgcgtcg
     1261 atccgcaccg ccaatggcgc tacgtcacca acgtctggca gaactcgcag attcgcactg
     1321 ctctgtacat gcccaccatc ccgttccgag ccgccgcccg ccgcttccgc taactagccg
     1381 agtccggggc cctgttcgtc cgtggtggcg gggctccgta cccgccttga gtggcacaac
     1441 cagatgtcgc acggagccgg actggattcc gacgcaatct ggggccagtt gatgcatgcg
     1501 aaccgttgcg tcgggcgagt cgaggcgggt acggagcccc gccactacga tgattcgttg
     1561 gtattccccg taccggcgca ccgaatctcc gctgctacag tcggctccac gtcgttcgcg
     1621 gacgggagag ccatgcagca cgaatccgaa tcacccatca ccccacgcaa ctccggcaaa
     1681 ccggacaccg gcaagcccga tgccgaggtc ttcgaggaag ccgacgccct cgacctccat
     1741 cagcagcgcc cgaccggtcc ggaggacggc ttcggcaagc tctggcgcaa gctccaccgc
     1801 atcgacctga ccggcgccga ccttccgccg gagcacgtca tcgccacctg gaagcagcac
     1861 ttcggagagt tctggccggg ccaaaaccgc ttctacgggc cgatcaccgc gctgcaaccc
     1921 ggcgagctgg ccgtcatcaa catcggggca ccgcccggta ccctctcgac cggcgtcaga
     1981 ctcgccgaat cgacgccgac ctccttcacg ctggtcacga ccaagacgca catgctctgc
     2041 ggctggctcc gtttctcggc cgaccgctca tccggggcca ccacggtctc agtagaaatg
     2101 ctgatccgcg ccagtgaccc catcttcgag atcggcatga tcttcggcgg gcatcgctgg
     2161 gaaaaccact tctggcggca aatgctgcgc aacctcgccg cccacttcgg catcgcggcc
     2221 gaaccggaat cccgggtcac ctgcgaagat ccccattacc gatgggagaa cgccaaaaac
     2281 atctggcaca atggggcgat tcggaacggc ctggtccggg tggccgcact cccgggcaag
     2341 gccagcgacc tcctccgtca tcgccgcaca gaaaggacct cgtgaccagc acccacgacg
     2401 ccgtcattat cggcgccgga cccaacggct tggccgccgg cattacgctt gcccaggccg
     2461 gccgctccgt cctgatcctc gaggcccgtg agaccgtcgg cggcggttgc cgcaccctcg
     2521 agctaaccct gcccgggtac tggcacgatc cctgctcggc gatccacccg cttgcggtcg
     2581 tctcgccatt tttccggtca ctcccgctcg cggattacgg cctcgagatg atcgagccgc
     2641 cgctcgccct cgccaatccg ttcgacgacg gcaccgctgc cgtcctcgcg aaatcagtcg
     2701 acgagacggc cgcctcgctc ggcccggacg gggacgctta caagcgactg ctcgacccgc
     2761 tgctcgagga ccaggagatc ctcatccccg agctgctcgg accgctccgg atcccgcccc
     2821 gtcatccgat cgcaatggcc cgcttcggtc tggtcggtct ccgctcggcc aaggggctcg
     2881 cgttttcccg cttcaaaggc cgcaacgcca gagcgctctt cgccggcatg gccggccact
     2941 cgatgctcaa gctcgaccag ctcgtgaccg gcgccttcgg tctgacactt gccctcttcg
     3001 gccacgttgc caactggccg gtcccgcgcg gcggctcgca ggcaattgtc gatgcgctcg
     3061 ccgcccactt ccgatcgctc ggcggcgaga tccggaccaa cacgccggct gactcgatct
     3121 cccaatacaa ggacactcac gcgattctct tcgacacgac gccgcgccag ttcctcaccc
     3181 tcgccggcga tcaggtcccc ggccgctacc gccgacacct tcagcactac cggtatggac
     3241 ccggcgtctt caaggtcgat tgggccctct ccggcccggt cccctggacc gcactcgagt
     3301 gccggcgtgc cggcaccgtc cacctcggcg gctcgtttga ggagatcgcc gcatccgaag
     3361 ccgacgcctg cgccggtcgc cactcggagc gcccctacgt gatcgtcgcc cagcagagcc
     3421 tgttcgactc gacccgtgcc ccggacggca accacacgct ctgggcctat tgccacgtcc
     3481 cgagcggctc cgacaaggat atgacggccg ccatcgaggc acagatcgag cggttcgccc
     3541 ccggcttccg ggacctggtc atcgcccgct ccgtgatggg accgggcgtg atcgaggccc
     3601 acaacccgaa ctacatcggc ggcgacatca acggcggcgt ccaggatatc cgccaactct
     3661 tcacgcgccc ggccgtccgc cgcgacccct actcgacacc aaacccgcgc ctctacctct
     3721 gctcctcatc gacccc
//
LOCUS       DATAOZ010000496         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8616558, whole genome shotgun sequence.
ACCESSION   DATAOZ010000496 DATAOZ010000000
VERSION     DATAOZ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8616558"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..434)
                     /locus_tag="VHR64_12780"
     CDS             complement(150..434)
                     /locus_tag="VHR64_12780"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX3303752.1"
                     /translation="MDMTTSRYLVERTFVDGLHIPISAEGEQACSLVNDQNSDLGVTW
                     VHSYVSEDKTKTYCVYDGPNPEAIRKAAERNSLPVDRITKISVLDPFFYR"
     gene            complement(545..>2565)
                     /locus_tag="VHR64_12785"
     CDS             complement(545..>2565)
                     /locus_tag="VHR64_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3303753.1"
                     /translation="NPFFVTEVLGSGMIGVPPTVCDAVLNRAARLSDSARAVLDLAAV
                     IGSRIEPGILTTVGNVDAGPVDECLAAGLLQVREGVIVFRHELAREALLSAVSPVRSM
                     ELYGRVLAALRAGHGTSDDLARLAHHAEAAGDVEAVLRFAPAAARRSSELGAHRAAAA
                     QFARAVRFAARLPDDERQELLRARLGACHLSGQTTESLDLATQLLVMARAAGDRIMEA
                     EYHSWLATIYVTAGRNVEADEASSTAIALLAELPQARSHAFVYCVQAQLRMLNRDRDE
                     AIRWGTRASELAGQLGDTEIRVRALNASGSARLVADAEDAQGRADLEESLEVARAANL
                     EPEVAGAFTNLGSSFGEGYRFTLAERYLTEGLAFTGERDLDRWHWYMVAWLALVRMYQ
                     GRWSEATELAQEVISNRGTTAISRIMALVALGRVRVRRGDPETWRILDEALALAEPTG
                     TLQRLAPVRSARAEAAWLAGDRDRTISEARAVFDLALKHRHQWHIGELAYRLWLAGDL
                     ASLPPGAARPFERQIAGDWSVAAKEWERLGCPFEAARALGESCDEAGLRASLAGFDQL
                     GARPMAAIASRHLREMGATSIPRGPRASTRENAARLTSRELDVLRLVARGQTNAEIAT
                     QLFLSPKTVKHHMTAILGKLGATSRRDAASLAAERGIIEPGEGRTVPI"
BASE COUNT          457 a          883 c          779 g          446 t
ORIGIN      
        1 aaacctgtct tgatcgtgac ccgccacact gctcgggtcc cggatttcct cagcgcaact
       61 cgttacctcc tccggtgacg ccccgaagta gacacctcgt gacaggccca cccactcttg
      121 ccatcggaga cgggcaagcg gccgactgat tagcgataga agaacgggtc cagcaccgaa
      181 atcttcgtga tccggtcaac cggcagcgag ttacgctccg ctgccttgcg gatcgcctcg
      241 ggattcgggc cgtcatagac gcaatacgtt ttggtcttgt cttcgctgac gtacgaatgg
      301 acccaggtaa cccccagatc gctgttttga tcgttcacca gggagcacgc ttgctcgcct
      361 tcggccgaga tcgggatatg gaggccatca acgaaggtgc gctcaaccag gtaccgggat
      421 gtcgtcatgt ccattgcctc tcctcattgg tgcgaaacgg ttcgccggcc ggcatgacac
      481 acgctatcgg ctgattgccg tcgccgcatc gggaggggac cctcatattg gtccggcggg
      541 atgcctatat tggaaccgtt cgcccttcgc ccggttcgat gatcccgcgc tccgcggcca
      601 gagatgcggc atcccggcgc gacgtcgcgc cgagcttgcc gaggatcgca gtcatgtggt
      661 gcttgacggt cttcggtgag agaaagagct gggtcgcgat ctccgcattc gtctgaccac
      721 gagcaacgag tcgcaagacg tccaactccc gggacgtaag ccgggcagca ttttcgcgag
      781 tcgaggcccg tggcccgcgc ggtattgatg tggctcccat ttcacgcagg tgccgggatg
      841 cgatcgcggc cataggtcgc gccccaagct ggtcgaatcc tgccaggctc gcccgcaacc
      901 cagcttcgtc acaactttca ccaagggcgc gcgctgcttc aaagggacag cccagcctct
      961 cccactcttt cgcggcgaca gaccaatcgc cggcaatttg ccgttcaaat ggcctggcag
     1021 cgccgggcgg aagcgacgcc aggtcacccg caagccagag ccggtacgcc aactcgccga
     1081 tatgccactg gtgacggtgc ttcagtgcga ggtcgaaaac ggctcgtgcc tccgaaatgg
     1141 ttcggtcgcg atcgcccgcc agccaggccg cttcggcccg cgccgaccgc accggagcca
     1201 ggcgctgcaa tgtgcccgtc ggctcagcca gtgccagcgc ttcatcgaga atcctccagg
     1261 tttcggggtc gccccgccgg acgcgaaccc ggccgagcgc aaccagggcc atgatgcggc
     1321 tgattgcagt cgtgcctcga tttgaaatga cctcttgcgc cagttccgta gcttcagacc
     1381 accgaccctg gtacatgcgg acgagtgcga gccacgcaac catgtaccag tgccatcgat
     1441 caaggtcgcg ctcgccggtg aatgccagac cctcggtcaa gtaccgctcg gcaagcgtga
     1501 agcggtaccc ctctccgaaa gaggatccca ggttcgtgaa cgcgccggcc acttccggtt
     1561 ccagattcgc ggcccgcgca acctccaggc tctcctcgag gtcagcccgg ccctgggcat
     1621 cttcggcgtc agccacgagt cgcgcgctgc ccgacgcgtt gagcgcccgc acccggatct
     1681 cggtatctcc aagctgcccc gcgagttcgg acgcccttgt tccccagcgg atcgcctcgt
     1741 cgcggtctcg gttcagcatt cgtagctggg cttgcacgca atagacgaac gcatgggacc
     1801 gggcctgtgg cagttcagcc aggagcgcga tcgcggtgct gctcgcctca tcggcctcga
     1861 cattccgccc ggcggtgacg tagatggtgg cgagccacga gtggtattcg gcctccatta
     1921 ttcgatcgcc ggccgcgcga gccatcacca ggagctgggt cgcgaggtca agcgactccg
     1981 tcgtttgccc gctcaggtga cacgcaccga gtcgcgcacg caagagctct tgccgctcat
     2041 cgtcaggcaa cctggccgcg aaacgtaccg cgcgggcaaa ctgtgctgct gccgcacgat
     2101 gcgcgcccag ctcggacgag cgccgagcgg cggctggggc aaatcggagg acagcttcca
     2161 cgtccccggc cgcctccgcg tggtgcgcca gccgcgcaag atcatccgac gtgccgtgcc
     2221 ctgcccgcag tgccgccagg acccttccat acaactccat gctccgaacc ggggatacgg
     2281 cgctgagcag cgcctcgcgc gcgagctcat gccggaagac gatgacgcct tcgcggacct
     2341 gaagcaaccc cgccgccagg cattcgtcga ccgggcctgc gtcgacattg ccgactgtcg
     2401 tcaagattcc cggctcaatc cgcgagccaa tgaccgctgc caagtccagc accgccctgg
     2461 cggaatccga tagacgggca gcacgattca ggaccgcgtc gcacacggtc gggggaacgc
     2521 cgatcatgcc ggacccgagg acttcggtga caaagaatgg gttgc
//
LOCUS       DATAOZ010000497         2866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6775165, whole genome shotgun sequence.
ACCESSION   DATAOZ010000497 DATAOZ010000000
VERSION     DATAOZ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6775165"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VHR64_12790"
     CDS             complement(<1..635)
                     /locus_tag="VHR64_12790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303754.1"
                     /translation="MTTETRVQPVRESPTARTIEAESLSASRARWLLLTVAVGTTLAP
                     LNSTMIAVALPDIQDAFGVSVTATAWLVTIYLVAMAVGQPIGGRLGDLYGRRRVYLFG
                     LAWFAIASAGCAFAPTLPVLIIFRTQQALAGALSFPNGAAMVREGIPPERRGSAFGTI
                     GMAASAAAASGPPIGGLLVHWFGWQSIFWMNVPVVGLAIALGLRSLPRMDSA"
     gene            complement(632..2320)
                     /locus_tag="VHR64_12795"
     CDS             complement(632..2320)
                     /locus_tag="VHR64_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX3303755.1"
                     /translation="MTVTDAPAGSPAFPVHWDDPADANFTYTTDMMHNPDPMSPLQQS
                     LQPSTFYGWQKAMREFGMPFKAMHVRFQNFYQYDRVEMIEPASPEEARAGGEALEATM
                     KVEVARLGDRWENEHLPRTRQIIDRFIAMEHDSATASLVDIAAMLDEFENLRAELWTI
                     HFRTVLPMMLAMQTYDELYADLFGGTEADAHILLGGRLTRSVAAGIGLSDLAATARAA
                     GLDRLIIETPLESLIQELRMSEDGRAVLDELGRYLDEFGYRSDLFDIMTPTWRENLLI
                     PLAAVRAYLMNGFNTSADHDAKARAADEALNAARGRLASYPEPVRQQFEAMVRAGRVA
                     ASLQEDHNFYIDQHSVAWTRLLFLQIGGRLAAAGVLDSRDDIFMIRLDEIRDLLSNPI
                     DDSIRGQTRKTVAERGAGIEHAMTLTPPPFLGPPPEAPTEDNVIVRGMDRFWGAPPRP
                     AEGPGQLMGNAGSRGVATGEAFVAHNLSDATNLQPGQILIAMTTMPAWTPFFGVAAAV
                     VTETGGPLSHCAVVAREYGIPAVVGAAGAMSAIQPGQRITVDGGRGIVLLNPDE"
     gene            complement(2400..>2866)
                     /locus_tag="VHR64_12800"
     CDS             complement(2400..>2866)
                     /locus_tag="VHR64_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX3303756.1"
                     /translation="GLVDDLAIAVIAQQLVPCDVSFVAFSADPLTGDEHRVLINAAWG
                     LGEAIVSGIVTPDNITVGLSGVILDYAIGDKETMIVADASAAEGTRQVPVPRAMRRLP
                     ALSDAQAATIATFARRLAVEFGHPIDLEGGLVGDRLHVFQARPITAFGSPVA"
BASE COUNT          490 a          884 c         1016 g          476 t
ORIGIN      
        1 gcggagtcca ttcgtgggag gctgcgcagt ccgagcgcga ttgccagtcc gacgaccggc
       61 acattcatcc agaaaatcga ttgccagccg aaccagtgga cgagcaagcc accgatgggt
      121 ggtccggatg ccgctgccgc gctggcggcc atgccgatcg tgccgaaggc ggagccgcgc
      181 cgctcgggcg gaatgccttc gcgcaccatt gccgcgccat tcgggaagga gagcgcgccg
      241 gcgagcgctt gttgcgtccg gaagatgatc aggaccggca gggtcggggc gaaggcgcag
      301 ccggccgatg cgatcgcaaa ccaggccaac ccgaagaggt agacgcgccg ccggccgtag
      361 agatcgccga ggcgacctcc gatcggttga ccgacggcca tcgcgaccag gtagatcgtg
      421 accagccagg ccgtcgccgt gaccgagacg ccgaaggcgt cctggatgtc gggcagggcg
      481 accgcgatca tggtcgagtt gagcggggcg agcgtggtcc cgacggcgac cgtcaacaac
      541 agccagcgtg cccgggaggc cgatagtgac tcggcctcga tcgtccgggc ggttggcgac
      601 tctcgaactg gttggacgcg ggtctcggtg gtcactcgtc tggattgagc agcacgatgc
      661 cgcggccccc gtccaccgta atgcgctgcc cgggctgaat cgcgctcatc gcgccggctg
      721 ccccgacgac cgccggaatc ccatattcgc gggccaccac ggcgcagtgg ctgagcggtc
      781 cgccggtctc ggtgacgacg gccgcggcga cgccgaagaa gggcgtccag gccggcatgg
      841 tggtcatggc gatcaggatc tggccgggtt gcaggttggt cgcatcgctc aggttatgag
      901 cgacgaaggc ctcgccggtg gcgacgccgc gggagccggc gttgcccatg agctgacctg
      961 gcccctcggc cggccggggc ggcgcacccc agaagcgatc catgccccga acgatcacgt
     1021 tgtcctccgt cggcgcttcg ggaggtgggc caaggaaggg cggcggggtg agcgtcatcg
     1081 catgttctat cccggcgcca cgctcggcga ctgtcttcct ggtttgccca cggatcgagt
     1141 cgtcgatcgg gttgctcagg agatcgcgga tttcgtcgag ccggatcatg aagatgtcgt
     1201 cccgggaatc caagacgccg gccgcggcca ggcgcccgcc gatttgcagg aaaagcaggc
     1261 gagtccaggc gacgctgtgc tggtcgatgt agaagttgtg gtcctcctgg agggacgcag
     1321 caacgcgtcc ggcccgaacc atggcctcga actgctggcg gaccggttcc gggtaggatg
     1381 ccaggcgtcc gcgcgcggcg ttcagtgcct catcggcggc acgtgccttc gcgtcatggt
     1441 cagcactcgt attgaagccg ttcatgaggt acgcgcgaac ggctgcgagc gggatcagga
     1501 gattctcgcg ccatgtcggg gtcatgatgt cgaagagatc ggagcgatat ccgaactcgt
     1561 ccagataccg accgagctcg tcgagaacgg cgcggccatc ctcggacatg cggagctcct
     1621 ggatcagcga ttccagcggg gtctcaatga tcaaccgatc cagcccggcc gcacgcgcag
     1681 tcgccgcgag gtcggacagt ccgatgccgg ccgcgacgct gcgggtaagt cgtccgccga
     1741 ggaggatatg cgcgtcggcc tcagtaccac cgaacaggtc ggcgtagagc tcgtcatagg
     1801 tttgcatggc cagcatcatc ggcaacacgg tccggaaatg gatggtccag agttctgcgc
     1861 ggagattctc gaattcatca agcatcgccg cgatatcgac aagggaagcg gttgcgctgt
     1921 cgtgttccat cgcgatgaag cggtcgatga tctgtcgggt acggggcagg tgctcgttct
     1981 cccagcggtc gcccaggcga gcgacctcga ctttcatagt ggcctcgagt gcctcgccgc
     2041 cggccctggc ttcctccggg ctggccggtt cgatcatctc gacccggtcg tattgataaa
     2101 agttctggaa ccggacatgc atcgccttga acggcatccc gaattcccgc attgccttct
     2161 gccagccgta gaacgtgctg ggctgcagtg attgttggag cgggctcatc ggatccgggt
     2221 tatgcatcat gtcggtggtg taggtgaaat tggcgtccgc cggatcatcc caatgcaccg
     2281 gaaaggcggg actgccggct ggggcgtcag ttacggtcat cgagggctgc tcctctcatc
     2341 tcatgacaca ttcgcgccag ggcggcgcga tgttgactcc gcttgccgag gtgattccgc
     2401 tacgcgactg gactaccgaa ggccgtgatt gggcgagcct ggaagacgtg gagacgatcg
     2461 ccgacgagtc cgccctcgag gtcgatcggg tggccgaact cgaccgcgag ccggcgggcg
     2521 aaggtggcga tcgtggcagc ctgggcgtcg gagagggcgg gcaggcggcg catggcccgc
     2581 ggcaccggga cctggcgcgt cccctccgcg gccgatgcgt cggccacgat catcgtctct
     2641 ttgtcgccga tcgcgtaatc gaggatcacg ccggagaggc cgaccgtgat gttgtccggc
     2701 gtgacgatgc cggagacgat cgcctcaccg aggccccacg cggcattgat gagcacgcgg
     2761 tgctcgtcgc cggtgagggg atccgcggag aaggcgacga acgagacgtc gcacggaacg
     2821 agttgctggg cgataacggc aatcgcgagg tcgtcgacga ggccgg
//
LOCUS       DATAOZ010000498         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3617157, whole genome shotgun sequence.
ACCESSION   DATAOZ010000498 DATAOZ010000000
VERSION     DATAOZ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3617157"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VHR64_12805"
     CDS             complement(<1..712)
                     /locus_tag="VHR64_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX3303757.1"
                     /translation="MGALPIQDDRLVWLDGACHGRDLVGGKGASLSRLFSLGAPVPPA
                     ITFTTHAYRDLAAHLALASRLAQLATGTQNAVRQAIAETPLPGSVEHTIRHGWRILRE
                     RIGPESRIAVRSSAIDEDGGGFSFAGLHDTVLGVTTEEAFVAAVRRCWASLWTDRSIA
                     YRRSSGLVNDIAIAVIAQQLVPCDVSFVAFSADPVSGNQDHAVINAAWGLGEAIVSGI
                     VTPGNITVGLSGVILDYAI"
     gene            complement(889..1650)
                     /locus_tag="VHR64_12810"
     CDS             complement(889..1650)
                     /locus_tag="VHR64_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX3303758.1"
                     /translation="MNRDQICLQLYTVRTKATTDFLGTLRAVAEMGYPAVEFAGLHGH
                     TAAEVRAELNASGIKAPSAHVPYATLLTDAEGVCSDLHTLGCQYAIVPWIGEEHRNSM
                     EVAKQFVATLNPIAQEVAQCGLQLGYHNHDFEFAPIPGGDGTTLWDLIMAETDPSLVL
                     LQLDCYWSTYGGADTLGLLRASPRRYPLLHFKDMQGEGADRHDAPIGTGHMDLRTIID
                     TTRETTEWYIVEQDNPEDPLADAQISLKGMQSLAG"
     gene            complement(1691..>2312)
                     /locus_tag="VHR64_12815"
     CDS             complement(1691..>2312)
                     /locus_tag="VHR64_12815"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3303759.1"
                     /translation="GPSGWRQYTGDPAVFYTAGVGPLIDTGVYVLHAITGLLGPVKRV
                     QAFGGIVIPKRTVLIERLLGQEIEVGTNDQMLIHLDFGNNVFAQILSSFAVPRTKMPA
                     LELHGTGGTVSFSVQEWYNANGPVDLFIRDESPLGIEDWQTVQPPVASPHVNLIGAGV
                     PHFIGCLQGEEEPILTAEHACHVLDVMIAAGKAAAFGESISLETTF"
BASE COUNT          392 a          679 c          838 g          403 t
ORIGIN      
        1 cgatcgcgta atcgaggatc acgccggaga ggccgaccgt gatgttgccc ggcgtgacga
       61 tgccggagac gatcgcctca ccaagacccc aggctgcgtt gataacggcg tggtcctggt
      121 tgccggagac aggatcggcg gagaaggcga caaatgagac gtcgcagggg acgagttgct
      181 gcgcaataac ggcgatcgcg atgtcgttga ccagtcctga ggagcggcgg taggcgatcg
      241 agcggtcggt ccagagtgag gcccagcagc ggcggaccgc agccacaaat gcctcctcgg
      301 tcgtgacacc gaggaccgtg tcgtggaggc cggcaaagga gaagccgccg ccatcctcat
      361 cgatcgccga ggagcggacg gcgatccggg actcggggcc gattcgctcg cgaaggatcc
      421 tccagccgtg gcggatggtg tgctcgaccg agccgggtaa gggcgtttcg gcgattgcct
      481 gacggacggc gttctgcgtg ccggtggcga gttgggcgag gcgcgaggcg agggcgagat
      541 gggcagcgag gtcgcggtag gcgtgggtgg tgaaggtgat tgccggggga accggagcgc
      601 cgagcgagaa gagccggctc aggctcgcgc ccttgccacc gaccaggtcc cggccgtgac
      661 aggcaccatc gagccaaaca agtcgatcat cctggatcgg cagtgcgccc atcgcagaac
      721 cccagcgcga ttgttgtcaa aatattaacg ccaaccatca tacaccacgg ccaatgacgg
      781 accgtgaagt ttcggaattc ggcggattcc atagggcggt acgcgcgttt tggcgcatcg
      841 gattgccgga gttacgcggc atcgcccggg ttacatgtcg ggtggtggtt agccggcgag
      901 ggattgcatg cccttgaggc tgatttgggc gtcggcgagc gggtcttccg gattgtcctg
      961 ctcgacgatg taccactcgg tcgtttcccg ggtggtatcg atgatcgtgc gcaggtccat
     1021 gtggccggtg ccgatcgggg cgtcgtggcg gtcggctcct tcgccctgca tgtccttgaa
     1081 gtggaggagc ggataccgcc tgggcgaggc cctgagcaac ccgagggtgt cggcgccgcc
     1141 gtaggtcgac cagtagcaat cgagctggag caggacgagg gacggatcgg tctcggccat
     1201 gatcaggtcc cagagcgtgg tcccgtcgcc gccggggatc ggcgcgaact cgaagtcgtg
     1261 gttgtgatag ccgagctgga gaccgcactg cgcgacctcc tgggcgatcg ggttgagcgt
     1321 ggcgacgaat tgtttcgcga cctccatgct gttccggtgc tcctcgccga tccagggcac
     1381 gatcgcgtac tggcagccaa gcgtgtggag gtctgagcag acgccctcgg cgtcggtcag
     1441 cagggtcgcg tacgggacat gcgcgctcgg cgccttgatc ccgctggcgt tcagttcggc
     1501 ccggacctcg gccgcagtgt ggccgtggag accggcgaac tcgacagccg ggtagcccat
     1561 ctcggcaact gcccgaagtg tgccgaggaa atcggtggtt gctttggtgc ggacggtgta
     1621 gagctgcagg caaatctggt cgcggttcat tggttccctc cccgtgtcga atgaatcgtt
     1681 ggatgccgcg ctagaaggtc gtttcgagtg atatggattc gccgaaggcc gctgccttgc
     1741 cggccgcgat catcacgtcg aggacatggc aggcgtgctc cgcggtcagg atcggctcct
     1801 cctcgccttg caggcaaccg atgaagtgcg gcacaccggc gccgatcagg ttgacgtgtg
     1861 ggctggcgac cggcggctgg accgtctgcc agtcctcgat cccgagcggc gactcgtccc
     1921 ggatgaagag atcgaccggg ccgttggcgt tgtaccactc ctgcaccgag aagctgaccg
     1981 tgccgccggt cccgtgcagc tcaagcgcgg gcatcttcgt ccgcggcacg gcgaagctgg
     2041 agaggatctg ggcaaatacg ttgttgccga agtcgaggtg gatcagcatc tggtcgttgg
     2101 tgccgacctc gatctcctgt ccgagcagcc gctcaatcaa caccgtgcgc ttcgggatga
     2161 cgatgccgcc gaaggcctgg acccgcttga ccggcccgag cagaccggtg atcgcgtgga
     2221 ggacgtagac gccggtgtcg atcagcgggc cgacgccggc ggtgtagaag acggccggat
     2281 cgccggtgta ctgccgccag ccggacgggc cc
//
LOCUS       DATAOZ010000499         5560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_723386, whole genome shotgun sequence.
ACCESSION   DATAOZ010000499 DATAOZ010000000
VERSION     DATAOZ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723386"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VHR64_12820"
     CDS             complement(<1..416)
                     /locus_tag="VHR64_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX3303760.1"
                     /translation="MSLRIGVSSGAFYPIVPTENVPARAAELGISDIELLLQTAGEYS
                     PRFLRQVRDEADACGVAVHSLHLYQALHPLFTSYQRRTDEAIDLFRRVLEGAHITGAH
                     VVVWHGASRAEATAPDAWDQFIVMTARLAELAAEAGV"
     gene            578..1651
                     /locus_tag="VHR64_12825"
     CDS             578..1651
                     /locus_tag="VHR64_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303761.1"
                     /translation="MPVLLNRRTLIAGVGLTIIARPADLRAATEAAFTITEFPADLSN
                     LSGTYGRAINRKATIVGIATGELGPAAVRSRGKTAWNLPPSDQPSLANAINDVGLIAG
                     SVNDLAAVWEDDEPRMLATHGDDRTTAYAINADGIVVGSADKGAKSGVALRWTGKKVV
                     ELPSLGGASSRALGINADGVIIGYSSQDDAGDLVRAVRWVDDKIEEIGTLGGEISQAM
                     AINNHGHIVGSSTSEKGFSAVDHAFRHVDGEMSRLARLGKVKIRGRSGTIKLDRSVAV
                     GVNDDGAICGFSVSASENDPVSVATLWLGDEVLDLNAAIGKANREIVLTSADGVNRDR
                     DLVCTGYLLDDPQIPRLFRLEPA"
     gene            complement(2020..2739)
                     /locus_tag="VHR64_12830"
     CDS             complement(2020..2739)
                     /locus_tag="VHR64_12830"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX3303762.1"
                     /translation="MFPIGDENRGIRSRPYVNYAIIAINIVVFLYQLTLTEPDLSRFI
                     VDWGAVPWEISRGNDLITLITSMFMHGGWLHIAGNMLFLWVFGDNVEDTMGHVKYLIF
                     YLICGLAAGGLQIAINPDSQTPLIGASGAISGVLAAYLVLFPHGKIRTILMLGIPILV
                     LVPAWGMIGYWIVIQFINGIASLGVQTRETSGVAYFAHIGGFIAGAALVLLFRDRDIH
                     QQQLDARANNRAFQRTPLINR"
     gene            complement(2759..2905)
                     /locus_tag="VHR64_12835"
     CDS             complement(2759..2905)
                     /locus_tag="VHR64_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303763.1"
                     /translation="MIDKAQAKMKFFVYGMLLGLAFAPYSGKENRSRVMGWFATSVRD
                     VVKF"
     gene            complement(2902..3198)
                     /locus_tag="VHR64_12840"
     CDS             complement(2902..3198)
                     /locus_tag="VHR64_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX3303764.1"
                     /translation="MTSSKHRAAFVFGVLVGASAGVAAAFLNAPQSGRRTRDQIQQSV
                     ERVLFKALDMVPFQNVPTDTMTSTRPVGVPLVETPPVDVLIGSRPSEMGMTHTP"
     gene            3440..>5560
                     /locus_tag="VHR64_12845"
     CDS             3440..>5560
                     /locus_tag="VHR64_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3303765.1"
                     /translation="MASAQSSTATTPPMHPEAVLPRLTRLELVGFKSFAAKTVFQFER
                     GITAVVGPNGSGKSNIADAVRWALGETSYSSLRSKKTEDVIFAGGAGKAPAGMAEVTL
                     TFDNSTAWLPIDFAEVTVTRRAFRSGETAYLINGRKVRLKDIHRLTASLGQSYTVVGQ
                     GLVDAALSQRAEERRGLFEHAADLTGLRLKAAEAERNLAETQSNTDRLTDLLAEVEPR
                     LRSLERAARQANEYREVRDRLRMLQRGHFRRLLLDVTERLATVEQAAQIGESTAESVQ
                     SRHDDLTSRAAALRSERDDAVDVLEQHRAGLQATAEQLRRVLHEREIAGERLAALVRR
                     REDMAETQQGLEEQITGVLADLEDVRAQLAAADSAAAASREERDKADAAMAAERAGRA
                     AVEREHARLLALVTDRERQIADASRRKALLVQRAETDAAERERANQSAGDRREQLDRL
                     TADISALQQAESAAGQRLTDIDDEIAEIAAGTRAAHTEIEHAREQVRTVERAIAESSA
                     RLRELRRIHESGAGLFAGVRAVVQAARAGTLAGVRGTLAELIQLPASFDTAMEVALGG
                     HLQDIVVDAWADAETAIRYLKKQNAGRATFQPIDTVRGRRPPADALRDIRGLKGVHGV
                     ASELLTVPADLEGIINALLGRSLVVDDLDVARACLPHLPSGWNAVTLTGEIARTAGSV
                     TGGAAVRESGALGRERELRELPDEI"
BASE COUNT          994 a         1840 c         1826 g          900 t
ORIGIN      
        1 acgccggcct cggcggcaag ctccgccagg cgggccgtca tgacgatgaa ctggtcccag
       61 gcgtccggcg cagtcgcttc tgcccggctc gccccgtgcc agaccacgac gtgcgcaccg
      121 gtgatatgcg cgccctcgag aacgcgccgg aagagatcga tcgcctcgtc ggtccggcgc
      181 tggtaactgg tgaagagtgg atggagcgct tggtagaggt gcagggagtg aaccgctact
      241 ccgcaagcgt cggcttcgtc cctaacctgg cgcagaaacc tcggcgagta ctcgccggcg
      301 gtctggagca gcagctcgat gtcgctgatg ccgagttccg ccgctcgcgc cggtacattc
      361 tcggttggaa caatcgggta gaacgcgccg cttgacacgc cgatgcgcag actcatggac
      421 acgcagccct gcccacaatt gcgggccggc gtggccggta tcccgggcgg cggactatca
      481 tggtgagcgt cccgattgtt tcgcgctgtc gtgaaatgcg attcatgatg gcatcctagt
      541 gcattggtag agtcgcttga atccaatcag ttcccgcatg cccgtcctgc tcaatcgccg
      601 caccctcatc gccggcgtgg gcctgacgat catcgcccgc ccggccgacc tccgcgccgc
      661 gactgaggcc gcattcacta ttaccgagtt ccccgccgac ctctccaacc tctccggcac
      721 ctacggccga gcgatcaacc gcaaggcgac gatcgtcggg atcgcgactg gtgagttggg
      781 tccggcagcg gtccgctcgc gcggcaagac ggcctggaat ctgccgccct ccgatcagcc
      841 gagtctggcc aacgcgatca atgacgtcgg gttgatcgcc ggctccgtca acgacctggc
      901 cgccgtctgg gaggatgacg agccgcgcat gctggccacg cacggagacg accgtacgac
      961 cgcctacgcc atcaacgccg acggcatcgt ggtcggctcg gccgacaaag gcgcgaagag
     1021 cggcgtcgcc ctgcgctgga cgggcaagaa ggtggtcgag ctgccctcgc tcggcggcgc
     1081 atccagccgg gcactcggca tcaatgccga tggggtcatc atcggctact ccagccagga
     1141 cgacgccggg gatctggttc gcgccgttcg ctgggttgac gacaagatcg aggagatcgg
     1201 cacgctcggt ggcgagatca gccaggcgat ggccatcaac aaccacggcc acatcgtcgg
     1261 ctcctcgacc agcgagaagg gtttcagcgc ggtcgaccat gccttccggc acgtcgatgg
     1321 cgaaatgagc cggctggcgc gactgggcaa ggtcaagatt cgcggccgct ccggcaccat
     1381 caagctcgat cgctcggtcg cggttggcgt caacgacgac ggcgcaatct gcggcttctc
     1441 ggtcagcgcg tcggaaaacg acccggtctc tgtcgcgacg ctctggctcg gcgatgaggt
     1501 gctcgatctc aacgccgcga tcggcaaggc caaccgggag atcgtcctga ccagcgccga
     1561 cggcgtcaac cgggaccgcg acctcgtctg caccggctac ctgctcgacg atccgcaaat
     1621 cccgcgccta ttccgcctgg agccagcctg acgaggttac ggacgtagtg cgtgacctca
     1681 gcgccgcgca ttcatcgggg aagagcgagc atctcatgga ctccgggtgt tcggcgccgc
     1741 ccggggataa atgcccgggc tgaccaacgg gaagcccact aaaggggctg acagcggacg
     1801 cgtgctattc aaacggtcga tcctcggtgc ccggactgaa ggccggggct gaaccaccat
     1861 aaggcggctg aagccgacac tttttcagtc cctttagtgg actcattgcg acttagcccg
     1921 gacctttagc tccgggcggt accggtcggc cggttcccct atgcacgatg gtcctgcccc
     1981 acgaaattgc ccagcgctat gtttcgctgg ccagtgcgtc tatcgattga taagcggcgt
     2041 gcgttggaag gcgcggttgt tggcacgggc gtcgagctgc tgctgatgaa tgtcgcggtc
     2101 ccggaagagt aggacgagcg cggccccggc gatgaagccg ccgatgtggg cgaagtaggc
     2161 cacgccgctc gtttcccggg tctggacgcc gagcgaggcg atcccgttga tgaactggat
     2221 aacgatccag tacccgatca ttccccaggc cggcaccagc accaggatcg ggatgccgag
     2281 catgaggatc gtgcggatct tgccatgcgg gaacaacacc aggtaggcag ccaggacgcc
     2341 cgagatggcg cccgacgcac caatcagtgg cgtctggctg tccgggttga tcgcaatctg
     2401 caatccgccc gcggcgagcc cgcagatcag atagaagatc aggtatttga cgtgccccat
     2461 cgtgtcctcg acgttgtcgc cgaagaccca gaggaagagc atgttgccgg caatatgcag
     2521 ccagccgccg tgcatgaaca tgctcgtgat cagcgtgatc aggtcgttgc cccggctgat
     2581 ttcccacggc accgcgcccc agtccacgat gaaacgggac aaatcgggct ccgtaagcgt
     2641 caactgatag aggaatacaa cgatgttgat cgcgatgatg gcgtagttga catagggacg
     2701 ggaacggata ccgcggttct cgtcaccgat cgggaacatg ccttgtcaga cgttcaggtc
     2761 agaacttgac gacgtcacgg acactggtcg cgaaccaccc catcacgcgc gagcggtttt
     2821 ccttgccgct atagggcgcg aaggcgagac cgagcagcat gccgtagaca aagaacttca
     2881 tcttggcttg ggctttgtcg atcatggcgt gtgcgtcatc cccatttcac ttggccggct
     2941 gccaatcagg acatccaccg gcggcgtctc gaccagcggg acgccgaccg ggcgggtgga
     3001 ggtcatggta tcggtcggaa cgttctgaaa cggcaccatg tccagcgcct tgaagagcac
     3061 gcgctcgacc gattgctgga tctgatcccg cgtgcggcga ccggactggg gcgcattcag
     3121 gaatgcggcc gccacgccgg cgctggcgcc gaccagcacg ccaaatacga atgcggctcg
     3181 gtgcttcgat gaggtcatac gggtgctctc ccgggtgaat cagacgggtt gacacgagtc
     3241 acatgcgcat cctaccagcc agcctaaccg aaacgccaag aatcggccga agtctggcga
     3301 caattcggcc tcacccgccc tgctgcggta ccattgcgga acgatcagtc tgaccggaca
     3361 ggtgaaccga ttcagtatcc atcggttcgt atttcctgtg ccgcgcagat tcagaccact
     3421 cgaggattcg acagacccga tggcatccgc ccagtcgagc acggccacca cgccgccaat
     3481 gcatccagag gctgtgctgc cccgcttgac gcgcctcgag ctggtcgggt tcaagagttt
     3541 cgccgcaaag acggtcttcc agttcgagcg cggcatcacc gccgtcgtcg gaccgaacgg
     3601 gagcggcaag tcgaatatcg ccgacgccgt gcgctgggcc ctcggcgaga ccagctacag
     3661 ctcgctgcgc agcaagaaaa cggaagatgt catcttcgcc ggcggcgcgg gcaaggctcc
     3721 ggccgggatg gctgaggtca cgctcacctt cgacaactcg accgcctggc tgccgatcga
     3781 tttcgccgag gtcacggtca cccgccgcgc cttccgctcc ggagagacgg cctatctgat
     3841 caatggccgc aaggtccgcc tcaaggacat ccaccggctg accgcctcgc tcggccagag
     3901 ctatacggtg gtcgggcagg gtctggtcga tgccgccctc agtcagcgcg ccgaggaacg
     3961 ccgtggtctc ttcgagcacg cggccgacct gaccggtctg cgtctcaagg cagccgaggc
     4021 cgagcggaat ctggccgaga cccaatccaa caccgatcgg ttgaccgatc tcctggctga
     4081 ggtcgagccg cgcctgcgct cgctcgagcg cgccgcccgc caggccaatg agtaccgcga
     4141 ggtgcgcgac cgcctccgca tgctccagcg cggtcatttc cgccgcctcc tgctggacgt
     4201 caccgagcgg ctggcaacgg tcgagcaggc cgcgcaaatc ggcgagtcga ccgccgagtc
     4261 agtccagagc cggcacgacg atctcacatc tcgtgcggca gcattgcgct ccgagcggga
     4321 tgacgccgtc gatgtgctcg agcagcaccg ggccggcctc caggccaccg ccgagcagct
     4381 tcgccgcgtc cttcatgagc gcgagattgc cggcgagcgg ctggctgcgc tggtccggcg
     4441 ccgcgaagac atggccgaaa cccagcaagg tctcgaggag cagatcaccg gcgttctggc
     4501 cgatctcgaa gacgtgcgcg cgcagctggc cgctgccgat tccgcggcgg ccgcctcgcg
     4561 cgaggagcgg gacaaggccg acgccgcgat ggccgccgaa cgcgccggcc gcgcggcggt
     4621 cgagcgggag cacgctcgcc tgctggcgct ggtcaccgat cgggagcgcc agatcgccga
     4681 tgcctctcgg cgcaaggcgc tgctcgtcca gcgggccgag accgacgcag cagagcgtga
     4741 gcgggccaat cagtcggcgg gcgaccgccg cgagcaactg gatcggctga cagccgatat
     4801 atccgccctg cagcaggcag aatctgctgc tggccagcgg ctgaccgata tcgatgacga
     4861 gattgccgag atcgcggccg gcacacgcgc cgcccacacg gagatcgaac atgcccgcga
     4921 gcaggtgcgg actgtcgagc gcgcaatcgc cgagtcatct gcccggctac gcgagctgcg
     4981 ccgtattcat gagagcggcg ccggactctt cgccggtgtg cgcgcggtcg tccaggccgc
     5041 ccgggctggc acgctcgccg gggtccgggg cacgcttgcc gagctgatcc agctaccggc
     5101 cagcttcgac accgcgatgg aggtcgcgct cggcgggcac ctccaggaca tcgtggtcga
     5161 cgcgtgggcc gatgctgaga cagcgatccg ctatctcaag aagcagaacg ccggccgtgc
     5221 aacgttccag ccgatcgata ccgtccgcgg tcgccggcca ccggccgatg ctcttcgcga
     5281 catccgcggc ttgaagggtg tgcacggtgt ggccagcgaa ttgctgacgg tcccggcaga
     5341 tctcgaaggg atcatcaacg cgctcctcgg gcgatcgctc gtggtcgacg atctcgacgt
     5401 ggcgcgggcc tgtctgccgc acctgccgtc gggctggaat gccgtcaccc tgacgggcga
     5461 gattgcccgc acggccggat cggtcaccgg tggcgcggcg gtgcgggaga gcggtgcgct
     5521 cggccgggag cgcgagctgc gcgagttgcc ggatgagatt
//
LOCUS       DATAOZ010000500         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6248669, whole genome shotgun sequence.
ACCESSION   DATAOZ010000500 DATAOZ010000000
VERSION     DATAOZ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6248669"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VHR64_12850"
     CDS             complement(<1..962)
                     /locus_tag="VHR64_12850"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX3303766.1"
                     /translation="MSRTHLLAGAAIALGLTASGPASAQSYPAQSYPDRPIRLIVPFP
                     PGGPTDYVARLVAQHVSANFGQVILDNRPGAGGTIASKAVANADPDGYTLLYGSSATL
                     GIAPALYKNVEYDPIKSFAPVALVSRVPFVLGIATAVPANTLSEFIAYAKANPGKLNF
                     GATIGTPPHLVGELFKVTTGADIYYIPYKGAAQATTDLLAGQMHMTIEGATTLLAHIQ
                     SGKVRPLAVMSPQRLPALPDVPTMLENGYSGFPSASWTGVVAPAGTPAPIVGKLNAAI
                     NDALQSPEISTNFARFSAQAKVGSPQDFAAFIAVEAPKWAALVR"
     gene            complement(972..1256)
                     /locus_tag="VHR64_12855"
     CDS             complement(972..1256)
                     /locus_tag="VHR64_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303767.1"
                     /translation="MSEQSDQHRCDFCQSGHVTRYFRRIAFRQRTDKGYVSCSVTIPL
                     GVCDHCGSKHWTEEAEAIIEAAVRREYVKLVSRSLTLVSSGRVDTFAPDP"
     gene            complement(1493..>2269)
                     /locus_tag="VHR64_12860"
     CDS             complement(1493..>2269)
                     /locus_tag="VHR64_12860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX3303768.1"
                     /translation="IENRAGGGSSIGMRAAATAEPDGYTLLFASSGPLAITPALYKNL
                     DYDPNRKFVPVATVSSGPMVLVVSPTLQINNVQELVAYARANPGKLNYGATVGTPPHM
                     AWGLFTMLTGTDIVYVPYKGAAAAFTDLVAGQIQMNFDATGSLLPLIREGKLKALAVS
                     SASRNPDLPEVPTMVESGIDFLMSFWTAVLAPVGTPPDIVSKLNATINAGLGSAEMKA
                     TLAKFQVEAKAGSAQDFAAFLAAETKKWADVAKAANIKVE"
BASE COUNT          392 a          700 c          735 g          442 t
ORIGIN      
        1 tcccggacca gagcggccca tttcggggct tcgaccgcaa tgaacgcggc gaagtcctgc
       61 ggggatccga ccttcgcctg cgcgctgaac cgggcgaagt tcgtgctgat ctccggcgac
      121 tgcagggcat cgttgatggc cgcattgagc ttgcccacga tcggtgccgg cgtgccggca
      181 ggcgcgacca caccggtcca ggaggcggac ggaaatccgc tgtacccgtt ctcgagcatc
      241 gtcggcacat ccggcaaggc gggcagtcgc tgcgggctca tcaccgccaa gggtctgacc
      301 ttgcccgatt gaatgtgcgc gaggagggtg gtggcgccct cgatggtcat gtgcatctgg
      361 cccgccagca ggtccgtcgt cgcttgggcc gcgcccttgt acgggatgta atagatgtcg
      421 gcgccggtcg tgaccttgaa caactctccc accagatgcg ggggcgttcc gatggtggcc
      481 ccgaaattca acttcccggg attggccttg gcgtaggcga tgaattcgct caacgtattc
      541 gccggcactg cggttgcgat gccgagcacg aacggaacgc gcgagaccag cgccaccggt
      601 gcgaagctct tgatgggatc gtattcgacg ttcttgtaga gcgccggcgc gatgcccagc
      661 gtcgccgaac tgccgtaaag gagcgtgtag ccgtcggggt cggcgttcgc caccgccttg
      721 ctcgcgattg tgccgccggc gcccggccgg ttatcgagga tgacctgtcc aaaatttgcc
      781 gagacatgct gggcaacgag tcgggcaacg tagtcggtcg gcccgcctgg cgggaatggc
      841 acgatcaagc ggatcggccg gtccggatac gattgtgctg gatacgactg cgcgctggcg
      901 ggaccgctcg cagtcaggcc gagcgcgatg gcggcgccgg ccagcaggtg cgttctggac
      961 atgacggccc ctcacggatc cggcgcgaag gtatctactc taccggagct aactagagtc
     1021 aagctgcggg acacaagctt cacatactcc cgccgaactg cggcttcgat gatcgcctcg
     1081 gcttcctcgg tccagtgctt cgaaccgcag tggtcgcaga ctcccagcgg gatcgtgacg
     1141 ctgcaggaga catacccctt gtccgtccgc tgccggaacg cgatccggcg gaagtatcga
     1201 gtgacatgac cgctttggca gaaatcgcag cggtgttggt cgctttgctc ggacataggt
     1261 cgctcgagat ttgagatgag cctccggctc cgccctgcat cagcgtgtct gggtcgctca
     1321 ttggtcgctg gttaagacgc cgttcgttca actcccgata ggaaaatctg gtttcgtcga
     1381 gcgaaaaaac ggcttcaatt gggtttcgag ggacaaggaa cgggtttcga ccgcacgacc
     1441 gtatcggtcc cgggcgaagc ggcgccgccg cgaatttcgc cggggcgcat tgtcattcga
     1501 ccttgatatt tgctgccttc gcgacatccg cccatttctt tgtctccgcg gcgagaaacg
     1561 cggcgaagtc ctgtgccgat cccgcctttg cttccacctg aaatttcgcc agcgtcgctt
     1621 tcatctcggc cgaacccagg cccgcgttga tcgtggcatt gagcttgctg acgatgtcgg
     1681 gcggcgttcc caccggagcc agcaccgccg tccagaagct catgagaaaa tcgatcccac
     1741 tctccaccat ggtggggact tcaggcagat ccggattgcg ggacgcgctc gacaccgcga
     1801 gtgccttgag cttgccctcg cggatgagag gcaggagcga accggtggcg tcgaaattca
     1861 tctggatctg tcctgcgacg agatcggtga aagctgccgc cgcgcccttg tacgggacgt
     1921 agacgatgtc ggtgccggtc agcatcgtga acaggcccca cgccatgtgc ggcggggtgc
     1981 cgacggtcgc gccgtaattg agcttgcccg gattggccct ggcataggcg accaactctt
     2041 gcacgttatt gatctgcaat gttggcgaca ccaccagcac catcggtccg ctcgacacgg
     2101 tcgcgaccgg gacgaacttt ctgttcggat catagtcgag atttttgtag agcgccggcg
     2161 tgatcgcgag cggtcccgag gaggcgaaca agagcgtata gccgtcgggc tctgccgttg
     2221 ccgcggcccg cattccgatc gagctgccgc cgccggcgcg gttctcgat
//
LOCUS       DATAOZ010000501         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2041882, whole genome shotgun sequence.
ACCESSION   DATAOZ010000501 DATAOZ010000000
VERSION     DATAOZ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041882"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VHR64_12865"
     CDS             complement(<1..221)
                     /locus_tag="VHR64_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303769.1"
                     /translation="MTADLILAVNSGSSSLKVGLFTSDPEPVEIGSIVADGIGSDNGR
                     VRSDGQLRSGSTTEVELQTHDAAITLVLAR"
     gene            complement(218..943)
                     /locus_tag="VHR64_12870"
     CDS             complement(218..943)
                     /locus_tag="VHR64_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303770.1"
                     /translation="MPPVHPKFTAALQYCHETDTHRTLARFLTRRDLVHAQTGAQFHA
                     HLSLPHPPKECQMFTRIVVPLECNGADHAALDMAQDRARLYDCPIHLIRFVDVSHLVR
                     IGGHGPTLDLATCRTTLPAEHATAKDYVTAVANALTARGFQVTHEIRQGLLRFELPAA
                     LRPGDLVVTTIESNPGAIPQQADIANDPNLAAGGMVVIIVGPNQSKHFPQITIGHDFD
                     ECESVELPRRDSARIPTSPSTRR"
     gene            999..3587
                     /locus_tag="VHR64_12875"
     CDS             999..3587
                     /locus_tag="VHR64_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX3303771.1"
                     /translation="MQTTRPSTTNDPNDQSAQTRQANARDVQSGDLTGLSAAEVQDRR
                     DQGRTNAVSITNTRTYLEIVRRNAFTLINVVLYATCALLIAIGLYGDALVTVGLVLFN
                     VVISIVQEIRTKRTLDRISVLTWPKVTVRRDGQDRVVDPGELVQDDLLLIQPGDQIVA
                     DGPIVDGHGIEVDESLLTGEADPVDKSIGEKVLSGTFCVSGTGIYRAETVGQGSVANR
                     LTSGARAYRQSKTPMQQDMELIVRASILAVLLVGGPVLMDLVARAVAVVVRLVDQSGS
                     VAVREAFGGYPVENTVKSAAVILALVPQGLALMIAVTYAAAAMRLVGSGVLIQQTNAM
                     ESLSHVDVLCLDKTGTLTTNQLEVVEIHPLDASAEDAKQRAGDFAANSTSRNKTSDAI
                     ETAFSGQHRTVVGEVPFSSARKWSCVALDDDHGRGLYVLGAPDVLKPHLSKADEVAEI
                     VANWSGQGRRVVLLAGNPDPGSLRDDGDEPELPDLDPIGLIGLVDGLREESRETLRHF
                     SELGVNLKLISGDDPRTVAALAEQVGFPIKGEVISGLDLTSLSDEELAERARSGSVFG
                     RTTPDQKERLIQSLQRDGKYVAMTGDGVNDVPALKQANMGIAMRSGSDAARGVADLVL
                     LEDSFGALPRAFAEGQRIVAGMQDIAALFFVRSLYVMLILFGAAFVNMPFPLSPRDNA
                     LLATLTVGIPTLGLAAWAKPRPTRNRLLRPIARFSIPAALTIAPVCLVTFMIWWRIEH
                     SAEESQSILTATAVLCGLVLLPFVEPPVHWLSGGDEYSGDWRPTILAIALLGCFLIVN
                     FSSGLRRIFDFSQIDVGDLVALAAIVSVWAVFLRWAWRYRFFERLAGLEAWRGTKPAS
                     GSAPAE"
BASE COUNT          691 a         1134 c         1253 g          689 t
ORIGIN      
        1 cgcgcaagca ccaatgtgat tgccgcgtca tgcgtctgaa gctcgacctc ggtggttgat
       61 cccgaccgga gttgaccgtc cgagcgtacg cggccgttat ccgagccgat gccgtccgca
      121 acgatcgagc caatttcaac cggttcagga tcggacgtga ataacccgac cttcaggctg
      181 gatgagccag agttgaccgc caggatcaaa tcagccgtca ccgccgagtc gatggcgatg
      241 taggtatccg agccgaatca cggcgaggga gctcgaccga ctcacattcg tcgaagtcat
      301 gcccaatggt gatctgcgga aagtgcttac tctggttcgg ccccacaatg atcacgacca
      361 tgcctcctgc ggcgaggttt ggatcattgg cgatatcggc ttgttggggt atcgcgccgg
      421 ggttcgactc gatggttgtc acaaccaggt ctccgggtct gagcgcggcc gggagctcaa
      481 atctgagcaa cccctgtcgg atctcatggg tgacctgaaa gccgcgggcg gtgagggcgt
      541 tggctaccgc cgtgacatag tccttcgcgg ttgcgtgctc agcagggagc gtcgtgcggc
      601 aggtggccag atccagggtc gggccgtggc ctccgatccg caccaggtgg ctaacgtcga
      661 caaatcggat cagatggatt gggcagtcat agagccgcgc gcgatcctgc gccatgtcca
      721 gggcagcgtg atcggcgcca ttgcattcga ggggaacaac gatccgcgtg aacatctgac
      781 actccttcgg cggatgcggc agggagagat gagcgtgaaa ctgtgcgcca gtttgagcgt
      841 ggaccagatc ccgtctcgtc aggaacctcg ccaaggtccg atgagtgtca gtttcgtgac
      901 aatattggag tgccgccgtg aacttggggt gaaccggcgg caatgtcagt ctccacctca
      961 tgtccggttg ggcatcggtc gattgatgtg aggcccggat gcagacgaca cgaccctcga
     1021 caacgaacga cccaaacgac cagtcggcgc agacccggca agccaatgcc cgggacgtcc
     1081 agagcggcga cctgaccggg ctctcggcgg ctgaagtcca ggatcggcgc gatcagggac
     1141 ggacgaatgc cgtctctatc acgaataccc gcacctacct cgagatcgtc aggcggaatg
     1201 ccttcacgct gatcaacgtc gtgctctatg cgacgtgcgc gctcctgatt gccatcggac
     1261 tgtacgggga cgcgctggtt acggtcgggc tcgtcctctt caacgtggtg atctcgatcg
     1321 tgcaggagat ccggaccaag cggacactcg atcgaatttc ggtgctgacc tggccgaaag
     1381 tcaccgtccg gcgcgatggg caggaccggg tcgttgaccc tggagagctg gtccaggacg
     1441 acctgctgct catccagccg ggagaccaga tcgttgcgga cggtccgatt gtcgacggtc
     1501 atggcatcga ggtcgatgaa tccctcctga ccggtgaagc ggatccggtc gacaagtcca
     1561 tcggcgaaaa ggtgctctcc ggcacgttct gcgtctcggg gaccgggatc taccgcgcgg
     1621 aaaccgtggg acagggaagc gtcgcaaatc gtctgacaag cggcgcacgc gcctatcgac
     1681 agtccaaaac gccgatgcag caggacatgg agctgattgt ccgggcctcg atcctggcgg
     1741 tgttgctcgt cggcggtccg gtgctgatgg acctggttgc ccgagccgtc gcggtagtgg
     1801 tccggttggt cgatcaatcc ggctcggtgg ccgtgcgcga ggcattcggc ggatatccgg
     1861 tcgaaaacac ggtcaagtcg gcggccgtaa tcctggccct ggttccgcag gggctggcgt
     1921 tgatgatcgc agtgacgtac gccgcggcgg caatgcggct ggtcggcagc ggtgtcctca
     1981 tccaacagac gaatgccatg gagtcgctca gtcatgtcga cgtgctgtgc ctcgacaaga
     2041 cgggcacgct gaccacgaat cagctcgaag tcgtcgaaat tcatccgctc gatgccagcg
     2101 ccgaggacgc gaagcagcgc gcaggcgact tcgcagcgaa ctcaacgagc cggaacaaga
     2161 cgtccgacgc gatcgaaacc gcgttttcgg gccagcaccg cacggttgtc ggcgaggtgc
     2221 ccttcagctc ggcccgaaag tggagctgcg tagccctgga tgacgatcat ggacgtggct
     2281 tgtacgtgct cggcgcgccg gatgtcctga agccacacct gtcgaaggcg gacgaggtcg
     2341 ccgagatcgt cgccaattgg tcgggtcagg gcaggagagt cgtgctgctg gcggggaatc
     2401 ctgacccggg ttccctgcgg gatgacggag atgaacccga gctaccggac ctcgatccga
     2461 tcggcctaat cggacttgtc gacgggctgc gagaggagtc cagggagacc ctgcgccatt
     2521 tcagcgagct cggcgtcaac ctcaagctga tctcgggcga tgacccgcga acggtggccg
     2581 cgttggccga gcaggtcgga ttcccgatca agggagaggt catctcgggg ctcgatctga
     2641 cgtcgctgag cgatgaagag ctggctgagc gggcccgaag cggctcggtc tttggccgca
     2701 cgacgcccga tcagaaggag cggttgatcc agtcgctgca acgggacggc aagtacgtgg
     2761 ccatgaccgg cgacggcgtc aacgacgtgc cggccctcaa gcaggccaac atgggcatcg
     2821 cgatgcgcag tggcagcgac gcagcccgcg gagtcgccga tctggtgctt ctcgaggatt
     2881 cgttcggagc actgccaagg gcgttcgcgg aggggcaacg gatcgtcgcc ggcatgcagg
     2941 acatcgcggc gcttttcttc gtgcgctcgc tctacgtgat gttgatcctg ttcggagcgg
     3001 ccttcgtcaa catgcccttc cccctgtcac cgcgggacaa tgcgctgctg gcgacactca
     3061 ccgtcgggat cccgacgctg ggtcttgcgg catgggcaaa gccacggcca acccggaacc
     3121 ggcttctgcg gccgattgcg cgcttctcga tcccggcggc gctgacgatc gcgcccgtct
     3181 gcctggtgac cttcatgatc tggtggcgca tcgagcacag cgccgaagag tcgcagtcca
     3241 tcctgacagc caccgcggtc ctttgcggac tcgtgctgtt gccgttcgtg gagccgccgg
     3301 tccattggct ctccggtgga gacgagtaca gcggagactg gcggccgaca atcctggcga
     3361 tcgctctgct cgggtgcttc ctgatcgtca acttctcgtc cggcctgcgg cgcatcttcg
     3421 acttctcgca aatagacgta ggggatctcg ttgcgctggc agcgatcgtg tcggtatggg
     3481 cggtgttcct gcggtgggcc tggcgctacc ggttctttga gcggctggcc gggctggagg
     3541 cgtggcgagg tacaaagccg gcgagcggct cagccccggc cgagtgacgt cacttgctgg
     3601 cgatgcgccg cgcgccaaac aggatccagc acgacgcaag actgataacc gacattgcca
     3661 cccagaccgg agtgagcgtc catgcgccat cgacatcgct ggccgccgcg aagaatgaca
     3721 tcgagatcag ggcgatcgtg tatgccgccg ccttgatgac cagaatc
//
LOCUS       DATAOZ010000502         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5989398, whole genome shotgun sequence.
ACCESSION   DATAOZ010000502 DATAOZ010000000
VERSION     DATAOZ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5989398"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..607)
                     /locus_tag="VHR64_12880"
     CDS             complement(206..607)
                     /locus_tag="VHR64_12880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX3303772.1"
                     /translation="MTPLEKLKAMKMPFAELKGVEVVEAEKDRVVARMTVRPDLCTLH
                     HTIHGGAVMALADSVGAAATVINLPEDAKGTTTLESKTNFIGGAKEGTTVIATATPVH
                     RGRRTQVWSTRLETEDGKLVAVVTQTQLVLV"
     gene            727..1515
                     /locus_tag="VHR64_12885"
                     /pseudo
     CDS             727..1515
                     /locus_tag="VHR64_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
BASE COUNT          280 a          557 c          543 g          336 t
ORIGIN      
        1 agcccggaac gggcgtcgtg cgggtcgtgt cttcaagtac cacgtcaagt accacgtcaa
       61 aactcctctt ggtgtccctc gaggaggcgt tcgaatccct aggtccccaa tattgtgcgt
      121 cgcagcatat ttttcaagac gggaacctgc ccaagtttca ggcatcgaag gcgaccaatt
      181 cgttaacaaa atcaagcccc cgtagtcata cgaggaccag ctgggtctgg gtcaccacgg
      241 caaccagctt gccgtcctcg gtctccagcc gggtgctcca gacctgggtc cgccgtcccc
      301 gatggaccgg tgtagccgtg gcgataaccg tggttccctc cttcgcccca ccgatgaagt
      361 tggtcttgct ctcgagcgtc gtggtgccct tggcatcctc cggcagattg atcacggtcg
      421 cggcggcacc gacggaatcg gcgagcgcca tcaccgcgcc gccgtggatg gtgtggtgga
      481 gtgtgcagag gtcgggcctg accgtcatcc gcgccaccac gcggtctttc tcggcctcga
      541 cgacttcgac gcccttgagc tcggcaaacg gcatcttcat cgctttaagt ttctcgagcg
      601 gcgtcatcgg atctcctccc aattcattgt tgtttcagcg tgaattgctt cgggcggcaa
      661 agcaatgacg tccgaggtaa tggccctgct gacatcggcg acggcatttg cgcatatgct
      721 cttcccatgc gccacttccc gccccgccgc atcgtctgcc tgaccgaaga gacggtcgaa
      781 acgctgtacc tgctcggcga gcaggaccgc atcgtcggtg tctccggcta cgccgtccgc
      841 ccgccccagg tgcggcggga gaagccgcgg gtgtcggcct tcgtctccgc agacatcccg
      901 aagatcctgg cgctggaacc ggagctggtg ctggccttct ccgacctcca ggccggcatc
      961 gtcgccgatc tcgtgcgcgc gagcgtcgac gtccatgttt tcaaccagcg cgacatcgcc
     1021 gggattttgg cgatgatccg gacacttggt gcgctggtcg gcgccgctga acgcgccgaa
     1081 ggccttgccc gtgatctcga acggcgcctc gcagagatgg cggcaacacc tcgcccctcg
     1141 gcgcggccaa aggtctagtt cgaggaatgg gacgatccgt tgatctccgg catcggctgg
     1201 gtgtccgagt tgatcgagat cgccggcggt caggatgtgt ttcaggaatt gcgacatcgg
     1261 caggcggcaa aggatcgcat cgtctcggcg gaggcggtgc gcgaggcagc gcccgacgtg
     1321 atccttgcct cctggtgcgg caagaaggtc gtccctgccc gcatccgcgc gcgcgattgc
     1381 tggaacgaga tccccgccgt gcgcgacgac cgcatcgtcg agatcaagtc gccgatcatc
     1441 ttgcagccgg ggccggcggc gctgacggat gggctcgatg cgatcgtgac ggcgttgtgg
     1501 ggtagactca aatagcttga tcgcagctct cgtgtcccgg acgcgctgca acgcgtcagc
     1561 gttgctgcgc agagccggga cccatctcga tacggagttc agtgcagcgt gggccccggc
     1621 tctgcggcgc accgcaccgg acgatgcttc gcatcgccgg gggcgctgcg ctgcgtccgg
     1681 ggcacgagag cggaattcat ttcgtatgcc catccc
//
LOCUS       DATAOZ010000503         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9145807, whole genome shotgun sequence.
ACCESSION   DATAOZ010000503 DATAOZ010000000
VERSION     DATAOZ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9145807"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /locus_tag="VHR64_12890"
     CDS             <1..1897
                     /locus_tag="VHR64_12890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX3303773.1"
                     /translation="LINGSIARIAGLPGWIAFNLALGTVFAMSVTAAAGLAWSIVRST
                     FGQRLAIFAGAAAAFLVMIAGNMRAPIEYLRNSDYTWNAFWYQNIGWQSSRVIVDYGG
                     SLNGETIQEFPSFSWVLGDLHPHVLSLAYTIVALALAVSLFRRVHEEWDPLAWRSWPE
                     FIAVGVILGALYPMNSWDFPTYGVAIAVALLLSTGPSWDWIQQMAVIGIVAIIAWSPF
                     WVSFVPFAGPDSGNVTSIPGLHFIAKNVAGYRGERTSASEYLTVWGLTWTIAMIFLVV
                     ETVATWPPRVADSERPAQPPYVRGVVVASVIIIAMIALALPAPVIVLAGIPMALALRL
                     IWIRIDGARDLTMLVSVLYAAGWGITILTEFFYIQDVFHGRFNTLFKIYYQVWTMVGI
                     AGALALVLLWRRAAAATVPRAFLTAGMAVAIVAGLAYPIISIKTWFDYLNPTREWVGL
                     DGLAQHGTEAFVPNPANPNVNTGKSAADVAAIRWLYDHAEPGEVVLEAAGCSYTINTE
                     LPTGRFSTFTGIPTVIGWGGHEGQWRGGQPDLLDAIGPRAGDVSSMYGDPNPAQNPLF
                     DEYHITLMVVGDLEKYGAGLKDLDQPVCAVAGPYESINVPDYPGTGWELVYDGETKIY
                     RHTPQSP"
     gene            2035..2325
                     /locus_tag="VHR64_12895"
     CDS             2035..2325
                     /locus_tag="VHR64_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303774.1"
                     /translation="MVASYVDVSTYRDLGASARELDPSLESEPIHLDTLREMGWDVEC
                     RGCYWLVRNEHRRIELFRDGRVIFKDPTITALYQLDDEWLKVIRFVWPNVQF"
BASE COUNT          405 a          808 c          794 g          486 t
ORIGIN      
        1 cctgatcaat gggtcgatcg cccggatcgc gggattgccg ggctggattg ccttcaatct
       61 ggcgctcggc accgttttcg cgatgagcgt aaccgctgca gccggcctgg cctggagcat
      121 cgtgcggtcc accttcgggc agcggctggc gattttcgcc ggagccgcag ccgccttcct
      181 ggtcatgatc gctgggaaca tgcgcgcgcc gatcgagtac ctgcgcaaca gcgactacac
      241 ctggaacgcc ttctggtacc agaacatcgg ctggcaatcg agccgggtga tcgtcgatta
      301 cggcgggagc ctgaacggcg agacgatcca ggagttcccg agctttagct gggtgctggg
      361 cgatctccat ccccacgtcc tgtccctcgc ctacacgatc gtcgccctcg cgctggccgt
      421 ctcgctcttt cgccgcgtgc atgaggagtg ggatccgctg gcgtggcgct cctggccgga
      481 gttcatcgcg gtcggcgtga tcctcggcgc gctctatcca atgaatagct gggatttccc
      541 gacctacggt gtcgcgatcg cggtcgcgct cctgctcagc accgggccaa gctgggactg
      601 gatccagcag atggcggtga tcgggatcgt cgcgatcatc gcctggtcgc ccttctgggt
      661 cagctttgtg ccctttgccg gacccgactc cggcaacgtg acctcgatcc cggggctgca
      721 cttcatcgcc aagaacgtgg ccggataccg gggcgagcga acctcggcca gcgagtattt
      781 gacggtctgg ggtctgacct ggacgatcgc catgatcttc ctggtcgtcg agacggtcgc
      841 gacctggccg ccgcgcgttg cggactccga acggccggcc caaccgccct atgtacgcgg
      901 ggtggttgtc gcgtcggtga tcatcatcgc gatgatcgcg ctcgccctgc cggcgccggt
      961 aatcgtcctg gccgggatcc cgatggcgct ggcgctgcga ctgatctgga tccggatcga
     1021 cggcgcgcgc gacctgacga tgctggtcag cgtgctctac gccgccggct ggggcatcac
     1081 gatcctgacc gagttcttct acatccagga tgtcttccac ggccgcttca acacgctctt
     1141 caagatctac taccaggtct ggaccatggt cggcatcgcc ggggcgctgg cgctggtcct
     1201 gctctggcga cgagccgcgg cagcgacggt gccgcgggcg ttcctgaccg ccggaatggc
     1261 cgtggcgatc gtcgccggtc tggcctatcc gattatctcg atcaagacct ggttcgatta
     1321 cctgaacccg acgcgcgaat gggtcggact cgacggcctg gcccagcacg gaacggaggc
     1381 gttcgtcccc aatcccgcca accccaacgt caacaccggc aagtcggcgg cggatgtggc
     1441 ggcgattcgc tggctgtatg accacgccga accgggcgaa gtcgtgctcg aggcggccgg
     1501 ctgcagctac acgatcaaca ccgagctacc aaccggccgc ttctcgacct tcacaggcat
     1561 cccaactgtg atcggctggg gtggccacga ggggcaatgg cggggcggtc agccggacct
     1621 gcttgatgcg atcggaccgc gcgccggcga cgtctcctcc atgtacggag acccgaaccc
     1681 ggcccagaac ccgctcttcg acgagtacca catcacgctg atggtggtcg gcgatctcga
     1741 gaagtacggg gcggggctca aggacctcga tcagcccgtc tgcgcggtcg ccgggccata
     1801 cgagtcgatc aacgtcccgg actaccccgg aaccggctgg gagctggtct acgacggcga
     1861 gacgaagatc taccgccata ctccccagtc tccgtagcct gcgacactca agtagcgtta
     1921 cattttgctt tctgtagcac aggaggcttc tctttcggtc tccgcgtccc tagattaggt
     1981 atacggtcac ctgacaccgg ggacaggtgc gcccttttta acggagggtc tgtgatggtc
     2041 gcctcgtatg tggatgtgtc gacgtataga gatctggggg caagcgcgcg cgagctggat
     2101 ccgtcccttg agtcggagcc gattcacctg gacacgctgc gcgagatggg ctgggatgtg
     2161 gaatgccgtg gttgctactg gcttgtccgc aatgagcacc gccggatcga gctcttccgg
     2221 gacggccgcg taatcttcaa ggacccgacg attacggcgc tgtatcagct cgatgacgaa
     2281 tggctcaagg tgattcgctt cgtctggccg aacgtccagt tctgatcaac cgaatctcac
     2341 ctgctcgtag tggcggcgcg gctccggaca gcccgccgcc attacgctgt ctgcggaagg
     2401 gccacctgtt caccattgcc gaggaggttt ggtcgatgtc gcacaagctg catgccgccg
     2461 atccaactgc ccccctgaaa tcgatccggc gct
//
LOCUS       DATAOZ010000504         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3096853, whole genome shotgun sequence.
ACCESSION   DATAOZ010000504 DATAOZ010000000
VERSION     DATAOZ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3096853"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VHR64_12900"
     CDS             complement(<1..310)
                     /locus_tag="VHR64_12900"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX3303775.1"
                     /translation="MAFRKIAVLTSGGDAPGMNAAIRSVVRVAAASDVETLGVRSGFL
                     GLMDGNFVPLDLRSVGGIITRGGTILGSARALDFKTDEGRVRAVHQLERAGIDGLIVI
                     G"
     gene            complement(420..1049)
                     /locus_tag="VHR64_12905"
     CDS             complement(420..1049)
                     /locus_tag="VHR64_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303776.1"
                     /translation="MVTKLRLVETRDEAILRLAGLAKKRGIRVLTYPLTGEQFALSLS
                     HPETVHRVTRLGCDCLGFTYHNRCTHYAALLESLGELPPEQPGSPAAPAQVAAPVVLC
                     DACDEVMAHVNGVAFECACGGLYEIDWETADEVDVALAELSRQDPDRYDAVTRMLSDE
                     LTAGDTSREGRRSDAPAALGLYGVSPNEIAAVLIWRADLDSRSDDAIAA"
     gene            complement(1136..1603)
                     /locus_tag="VHR64_12910"
     CDS             complement(1136..1603)
                     /locus_tag="VHR64_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3303777.1"
                     /translation="MYKRVIVPLDGSELAEQALPNATELARASNASILLVSVVGYPYL
                     ERKGLTSWALQQEALDQVVREETGRAEEYLNGVRKRLVADGFDVTVEVRRGAVDQAIL
                     ADPEAGDVIVMTTHGRGGVARWFLGSVAEDVVRRSTVPVLLVRSQAAKEPATA"
     gene            complement(1690..2496)
                     /locus_tag="VHR64_12915"
     CDS             complement(1690..2496)
                     /locus_tag="VHR64_12915"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX3303778.1"
                     /translation="MEWISNPEIWTALVTLTALEIVLGIDNIIFISILAGKLPVNQRD
                     RARTIGLGAAMLTRIALLFSLNWVIGLTDPIFTVLDQEISGRDLILLIGGLFLLGKST
                     FEIHEKLEGEEVHGVARAAASFTSVIIQIMLLDIVFSLDSVITAVGMAKHIEVMVAAV
                     VISVGIMLVASGPISRFVDEHPTVKILALSFLLLIGMSLVAESFDQHISKGYIYFAMG
                     FSVLVEALNLRVRSKVEPVHLHNPHMNERFAGDARGTSEEAGEAAVTRMS"
BASE COUNT          510 a          877 c          819 g          485 t
ORIGIN      
        1 cgccgatcac gatcaggccg tcgattccgg cgcgctcgag ctggtggacg gcgcggaccc
       61 ggccctcgtc agtcttgaaa tccagggcgc gcgcactgcc gaggatcgtg ccgccgcgtg
      121 tgatgatgcc gccgacgctg cgcaggtcga gcggcacgaa gttgccatcc atgagaccaa
      181 ggaagccgga gcgaacgccg agcgtctcca catcggacgc ggccgcgacc cgaaccactg
      241 agcgaatcgc cgcgttcatg ccgggcgcat caccgccgct ggtgagaact gcgatcttgc
      301 gaaaggccac cgtgacttcc cttcccggcc gtgcgtcgca cctgatcaaa cgcgccgatc
      361 cgtcaatgga tcagcgcgct gcgactactt tacgcggtcg ggaatcagaa gatcacgcgt
      421 tatgccgcga tcgcatcgtc actgcggctg tccaggtcgg cccgccagat caggaccgcc
      481 gcgatctcat tcgggctgac gccatacaaa ccgagcgcgg ccggtgcatc tgagcggcgt
      541 ccttcgcggg acgtatctcc tgcggtcagt tcgtcgctga gcatacgcgt gaccgcgtcg
      601 tatcgatcgg gatcctggcg cgagagctcg gcgagcgcga cgtcgacctc gtcggctgtc
      661 tcccaatcga tctcatagag ccctccgcag gcgcactcga aggcgacacc gttgacgtgc
      721 gccatcacct catcgcaggc atcgcagagc acgaccggcg cggccacctg ggctggggca
      781 gccgggctcc cgggctgctc cggtggcaac tcgccgagcg attccagcag tgcggcgtaa
      841 tgggtgcagc gattgtgata tgtaaagccg agacaatcgc agccgaggcg cgttacccgg
      901 tggaccgtct cagggtggct tagcgaaagc gcaaattgct caccggtcag cggatatgtc
      961 agcacgcgga ttccgcgttt tttggccagc ccggcgagcc gaaggatggc ttcgtcgcgg
     1021 gtttctacca gtcgaagttt ggtcaccatg gcggcaatgc tccttcctga tgaagctcca
     1081 ttataccgca aaacgtacag atgttctagt cccggttttg cttttcaacc cgttatcacg
     1141 ccgtcgctgg ttcctttgcg gcctggctcc ggaccaggag gacaggcacc gtcgagcgcc
     1201 ggaccacgtc ctccgccacg cttccgagga accagcgggc gaccccgccg cggccgtgcg
     1261 tggtcatgac gatcacgtcg cccgcttccg gatcggccag aatcgcctgg tcaacagcgc
     1321 cccggcgtac ctccacggtg acgtcgaatc cgtcggcgac gagccgcttc cggacgccgt
     1381 tcaggtattc ctctgcccgc ccggtctcct cgcgcacgac ctgatcgagc gcctcctgct
     1441 gcaaggccca tgacgtcagg cccttgcgct cgaggtacgg atagccaacg acgctgacga
     1501 gcaatatgga cgcattcgaa gctcgggcaa gctcagtcgc atttgggagc gcctgctccg
     1561 ccagctccga tccatccagc ggcacgatca ctcgcttgta cattcacacc tccatcgatc
     1621 gcatcaccag atcacttgac ccgaccatat cgcaccccag atcgcgaatc cgcggcgggt
     1681 cgggcgccgc tatgacattc tcgtgactgc cgcctcgcct gcttcctccg atgtgcctcg
     1741 cgcatcgccg gcgaaccgct cgttcatatg cgggttgtgg aggtggaccg gctccacctt
     1801 gctgcgcacg cgcaggttca acgcctcgac caggaccgag aagcccatcg cgaagtagat
     1861 gtaacccttc gaaatgtgct ggtcgaacga ttccgcgacg agcgacatgc cgatgaggag
     1921 caggaagctg agggcgagaa tcttgaccgt gggatgctcg tcgacgaacc ggctgatcgg
     1981 tccggacgca accagcatga tcccgaccga aatgaccacc gccgcgacca tcacttcgat
     2041 gtgcttggcc atgccgacag ccgtgatgac ggagtcgagc gagaagacga tatccagcag
     2101 catgatctgg atgatgaccg aggtgaagga cgctgccgcc cgcgcgacac catgcacctc
     2161 ctcgccctcg agcttctcgt gaatctcgaa tgtgcttttg ccgagcagga agagaccgcc
     2221 gatcagcagg atcaggtcac gaccggaaat ttcctggtct aggaccgtaa agatcgggtc
     2281 ggtcaggccg atcacccaat tgagcgagaa gaggagcgcg atccgggtca gcattgccgc
     2341 cccgagccca atcgttcggg cgcgatcccg ctgattgacg ggcagcttgc cggccaggat
     2401 cgagataaag atgatgttgt cgatgccgag cacgatctcg agcgcggtca acgtgacgag
     2461 cgcggtccat atctcggggt tcgaaatcca ctccaaggcg tgatccttct ccgatggtcg
     2521 tcttgacggc cagggatggc tcgcgctcaa cccgggaatg cgtcgctgcg cagtggcggg
     2581 aattctacgg ggcgcacgcg aaatttcgtt ggtttctgcg acagcgtcga ttcgacgtag
     2641 acttcaaccg catcgctgac cattgagcat cgaatcccgg acaggacaat g
//
LOCUS       DATAOZ010000505         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_726025, whole genome shotgun sequence.
ACCESSION   DATAOZ010000505 DATAOZ010000000
VERSION     DATAOZ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726025"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1565)
                     /gene="purH"
                     /locus_tag="VHR64_12920"
     CDS             complement(33..1565)
                     /gene="purH"
                     /locus_tag="VHR64_12920"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX3303779.1"
                     /translation="MRAVLSVYDKAGLDEFAQGLADLGFDLVSTGGTHAHLARLALPV
                     RSVSDVTGFPEILDGRVKTLHPAIHGGLLARLDLPEHRAAIEEHGIEPIALLAVNLYP
                     FEATVNRPGCTIDEALEQIDIGGPAMVRAAAKNFPHVIVVTNPERYSDVLKALRDGAV
                     SDDMRRSLAGEAFQHVSTYDALVAEYLRGPNAEYPRELTIPGRIAYSLRYGENPHQSA
                     AAYLRPCMTGSQSGVLSSVQLQGKELSYNNILDADAALRATQTLAEPACSIVKHTIPC
                     GLAIRDTPAGAFEAALASDPVSAFGGIVALNRQVDAETAERLARIFLEVVVAPGFDVD
                     ARRILSAKKNLRLLELPGSAWEPSAESTIRTVQGGFLVQESDSSIDDPASWTVVTDTP
                     LPDELRADVALAWHAIRYVKSNAIVLSNDRSIVGVGPGQPNRVDSVRIAVQRAGDQCR
                     GSVLASDAFFPFADGVEEAIRAGVAAVIQPGGSMRDEEVVAACNNAGIPMIFTGTRHF
                     LH"
     gene            1826..>2838
                     /locus_tag="VHR64_12925"
     CDS             1826..>2838
                     /locus_tag="VHR64_12925"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303780.1"
                     /translation="MREAVAAYRSRTFKSLAVRNYRLYFIGQGISISGTWMQSVAQGL
                     LVLELTGSGTQLGLVTALRSIPVLAFGAMGGVIADRYPKRKILYASQSVAGLLGLTLG
                     ILVATGEVRMWMVGILAVMLGFVSVVDNPTRQSLILELVGPDQIRNAVSLNSTEVNLA
                     RVIGPTLAGILVSTVGLSACFIVDGLSYFAVIIMLMRMREEEMFLGQTVARARGQLRE
                     GFAYAWANLPVRKTLIMMAIIGTFTYEFNVILPIFAEFTFESGPSAYAAMTAAMGLGA
                     VVGGLSVAGRVRTSQWSIVISAALFGCSVLATALAPSLYVAIATLLVVGYFSISFTTM
                     GNST"
BASE COUNT          503 a          858 c          892 g          585 t
ORIGIN      
        1 gatcaatcgc gtcctcggac cggcctgatt cctcagtgca ggaagtggcg agtaccggtg
       61 aaaatcatgg gaataccagc gttgttgcac gcggcaacaa cttcttcatc ccgcattgac
      121 ccaccaggtt ggatgactgc ggcaactcca gcgcgaattg cctcctcgac gccatcggcg
      181 aacgggaaga aggcatcgct ggcgagaacc gaaccgcgac attgatcgcc cgcccgctga
      241 acggcaattc gaacgctatc gacgcggttc ggctggcccg gaccgacccc gacaattgag
      301 cgatcgtttg aaagcacgat cgcattggac ttgacgtaac ggatcgcatg ccaagccaat
      361 gcgacatccg cgcgcagttc gtcggggagc ggggtatcgg tcacgactgt ccagctcgcg
      421 ggatcgtcga tgctcgagtc gctttcctgg acaaggaatc ctccctggac ggttcggatc
      481 gttgactcag cacttggctc ccaggcgcta cccggaagct ccaggaggcg aaggttcttc
      541 tttgccgaaa ggatgcggcg agcgtcgacg tcgaatcccg gcgcgacgac gacctccagg
      601 aagatgcggg cgaggcgttc cgcggtttcg gcatcaacct gccggttgag cgcgacgatg
      661 cctccgaagg ccgagaccgg gtccgatgcc agcgctgcct caaacgcacc tgccggcgta
      721 tcccgaattg ccagtccgca cggaatcgtg tgcttgacga tcgagcaggc cggctctgcc
      781 agcgtctgag tcgcccgcag cgcagcgtcg gcgtcgagaa tgttgttgta cgacagttcc
      841 ttgccttgaa gttgtacgga gctgaggaca ccggactggc taccggtcat gcacggtcgc
      901 agatatgccg ctgccgactg gtggggattc tcgccgtaac ggagcgagta tgcgatccga
      961 cccgggatcg tgagctctcg cggatactcg gcgttcggac cgcgcaggta ttcggccacg
     1021 agtgcgtcat acgtggagac gtgctgaaac gcctcgcccg cgagtgaccg acgcatgtcg
     1081 tccgatacgg cgccgtcccg gagtgctttg agcacgtcgg aataccgctc cgggttggtc
     1141 acgacaatca cgtgtggaaa gttcttggcg gccgctcgaa ccatcgccgg tccgccaatg
     1201 tcgatctgct cgagggcctc gtcgattgtg cagcctgggc ggttgaccgt cgcttcaaac
     1261 ggatacaggt tgaccgcgag aagcgcgatt ggctcgattc cgtgctcttc aatcgctgcg
     1321 cgatgctcag ggagatcaag ccgcgccagt agcccgccgt gaatcgcggg gtgcagcgtc
     1381 ttgacccggc cgtcgaggat ctccgggaat ccggtgacgt cgctgacact tcggactggc
     1441 agagcgagcc tggcaagatg ggcgtgcgtg ccgccggtgg ataccagatc aaagccgaga
     1501 tcggccaaac cctgggcgaa ctcatccaat ccggccttgt cgtacacact gagcactgcc
     1561 cgcacgaagc gctgcctcct tcatcccgga ctcaatgccc ggattccctt caaccgcgtc
     1621 attgtaggtc cggtagtcag ttcggcgttc gctgcgacat cgactgcgtc aggggctgaa
     1681 atgttgcgac ccctgtagac ggtttgtgca tatagcgcga caatgcagac cttgttgagc
     1741 acggacctcc gcacgaaagc ctgtcaatgc cgctgtcagc gcctcctatc gtcgaccgcg
     1801 cgtcgtatgg ggaccattcc cggtaatgcg tgaagcggtt gctgcctatc gctcccgcac
     1861 attcaagtcg cttgctgtcc ggaactaccg cctctatttc atcggccaag gcatctcgat
     1921 ctctgggacc tggatgcaat cggtcgcgca agggttgctt gtcctcgagt tgactggttc
     1981 tggtacccaa ctcggactcg tgacggcgtt gcgatcgatt cctgtgctgg cattcggggc
     2041 gatgggcggg gtcattgccg accggtatcc caagcgcaag atcctctacg catcgcagtc
     2101 ggtcgccggg ctccttgggc tgacccttgg catcctcgtg gcaacgggcg aggtacggat
     2161 gtggatggtc ggaatcctgg cggtgatgct tggtttcgtt tccgtggtgg acaacccgac
     2221 acgccagtcc ctgatcctcg agctggtcgg cccggaccaa attcggaacg cggtttcgct
     2281 caactccacg gaggtcaatc tggcccgtgt gattggcccg acgcttgccg gcatcctcgt
     2341 cagcacagtc ggactctcgg cctgcttcat tgtcgacgga ctctcgtatt ttgccgtgat
     2401 catcatgttg atgcggatgc gggaagagga gatgttcctc ggccagacag tcgcccgtgc
     2461 ccgcggacaa ttgcgggaag ggttcgccta tgcctgggcg aacttgccgg tgcggaagac
     2521 gctaatcatg atggcgatca tcggcacctt cacgtacgag ttcaacgtga ttctgccgat
     2581 cttcgcggag ttcaccttcg agagcggacc gagcgcatac gccgcgatga cggcggcgat
     2641 ggggcttggc gcagtggtcg gcggcttgtc ggtggccggt cgggtgcgga cgtcgcaatg
     2701 gtcgatcgtt atctcagccg cgctctttgg gtgcagtgtt ctggccacgg cgctggcgcc
     2761 gtcgctctat gtcgcgattg ccaccttgct tgtggtgggc tacttctcga tcagcttcac
     2821 cacgatgggg aactcgac
//
LOCUS       DATAOZ010000506         4862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3620509, whole genome shotgun sequence.
ACCESSION   DATAOZ010000506 DATAOZ010000000
VERSION     DATAOZ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3620509"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VHR64_12930"
     CDS             complement(<1..1313)
                     /locus_tag="VHR64_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3303781.1"
                     /translation="MSDLFAFDDEMRQLLERAVQHAETRISHQHESLQSPPDPAALSG
                     SLGGAITTTGIGPAAAFDLFADVIAPNCLAVDNPRFLAFVAHPPSTAAQIMDMALSAS
                     SIFGTSWLEAAGATAAENQALRWLADLAGLPEGAGGVFLSGATIANLNALAAARAWWR
                     ENHPDHDRRLGVAMTHEAHASAKMVARVLDLEIVPVEGDACGRLTGPNLERALASATI
                     DVCAVVATGGITNVGIVDDLAGVADNCAARELWFHVDAAYGGAALAVAETKPLFAGIE
                     RAHSVVIDPHKWLFAPLDCSALLYKEPAHARAAFTQHADYIAAFQEIGDWNPGDYGVH
                     LTRRARGLPFWFVLATYGTDAVSAAVKTSLDLAKQTARMIEQRPELRLLMEPELSVVV
                     FTRNGWTMPDYRAWSDRLLADGTAFVLPTQVNGEPALRFCFVNPRT"
     gene            1513..1866
                     /locus_tag="VHR64_12935"
     CDS             1513..1866
                     /locus_tag="VHR64_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303782.1"
                     /translation="MDRTFDDIFDDIEAFVEERPVTARLMVGTVGATAVMMIAAILWS
                     TTDASASTMIIAAIGITIASWLIVSTALPIDCKIGPMHTRSLICRLQAQRRLVDVVAV
                     ALTVVVLLNIITTQV"
     gene            2020..3102
                     /locus_tag="VHR64_12940"
     CDS             2020..3102
                     /locus_tag="VHR64_12940"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX3303783.1"
                     /translation="MISVGRLDAAVELVDRFIGEGVPGAGLVVAEHGEIVGEYYAGMA
                     RPDRPADAETLWPLASISKLYAAAGCMAAIEEGRIALSTKASQIFPEFRQGGRDKITL
                     RHLLTHTSGLPYESDQMAERLAAKISLDEMVRESYEGDLLFAPGTDQSYSDYGIGLAG
                     LMCAKSMDLTFPDLVRSRVFDPGHLLDTYMPPPETEYHRIAHIVGPLAEKTDGAMYNS
                     AHARNLAHPAFGTIASARDLLQFGLLFDPNGSRSLFSRVATTTMITDQTGYDFPDAVT
                     APMSPAVRAWGLGFMVKGRMGFPELCSPAAFGHPGATGCMVLVDPTYDVAFAFVSNRH
                     LNTGYDEWHDRLMMITNVVMAGMTRG"
     gene            3042..4226
                     /locus_tag="VHR64_12945"
     CDS             3042..4226
                     /locus_tag="VHR64_12945"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEX3303784.1"
                     /translation="MARSFDDDHERRDGWYDPRITPLNRMPPAPRPGPESDLLLSQVI
                     RQMRRFGLGERVLKTALAAGLSWQLATLIPGNDHPYLAPLSAVLLMQLTIAQSVDLAI
                     QRNIGIAIGVIVAVASFALFGVHAWSVSLVVLISLAAGIQLRLGQQAVQQVAVSALIV
                     LLAGSVTGTFEYAFRRILDSIIGASVALVLNWLVVPPIHIAPASKAIERMAQDLSLAL
                     ADLAVSLRSGMTRTRAEAHLKSARALATSLAAANVALGQAEQSLRYNRFARGRHDEMA
                     RLQNASRALEHGAIQTRVLARSMVTAFEEDAADWIQPDQFGRSLADLLGRNAALVRYV
                     GGRGGIRPELAITTELHESMLAYWQARADPGWLYAGEVLAMTERMAKELNAAIDAGDT
                     RP"
     gene            4223..>4862
                     /locus_tag="VHR64_12950"
     CDS             4223..>4862
                     /locus_tag="VHR64_12950"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEX3303785.1"
                     /translation="MSTLNRRTQRQLQALTAQLTATEGPLRRALDELSRLGIGERIVK
                     SALAATIAWMVAAQLPRENAPFVAALTAVYTIDLTILKSIRSAWQRLAGIGLGIAMAF
                     LAAEFLGVHAWSVGLVILLSLVIGLRLNLKSDGMVQVAGTAIVVMVVRSTTEERSIYA
                     LIFLADTLIGTAIGLAVNSIFAPPVFVPAAWRAVTALGNRLIDLMDQLGTVVV"
BASE COUNT          778 a         1593 c         1646 g          845 t
ORIGIN      
        1 gttcttgggt tgacgaagca gaagcggagg gcgggttctc cgttgacctg ggtggggagg
       61 acgaaggcgg tgccgtcggc gagcaggcgg tcggaccagg cgcggtagtc cggcattgtc
      121 cagccattgc gcgtgaagac gacgacggag agctccggtt ccatcagcag ccttagttcc
      181 gggcgctgct cgatcatgcg ggccgtctgc ttcgccaggt cgaggctggt cttgaccgcc
      241 gctgagacgg cgtccgtccc atacgtcgcg agcacgaacc agaacgggag gccacgggcg
      301 cgccgggtga ggtggacgcc gtagtcgccg gggttccagt cgccgatctc ctggaaggcg
      361 gcgatgtaat cggcgtgctg ggtgaaggcg gcgcgggcgt gcgcgggctc cttatatagg
      421 agggcggagc aatcgagcgg ggcgaagagc cacttgtgcg ggtcgatcac gaccgagtgg
      481 gcgcgctcga tcccggcaaa gagcggcttc gtctcggcaa ccgccagggc tgcgccaccg
      541 taggcggcgt cgacgtggaa ccaaagctct cgcgctgcgc agttgtccgc cactccggcc
      601 agatcgtcga cgatcccgac gttggtgatc ccgccggtcg cgactaccgc gcagacgtcg
      661 atcgtcgcgc tggccagggc gcgttcgagg ttgggaccgg tcagacggcc gcaggcatcg
      721 ccctcgaccg ggacgatctc gaggtcgagc acccgggcga ccatcttcgc ggaggcgtgt
      781 gcctcgtgcg tcatcgcgac accgagacgg cggtcgtgat ccgggtggtt ctctcgccac
      841 cacgcccggg ccgcagccaa cgcgttgagg ttggcgatcg tcgcgccgct caggaagacc
      901 ccaccggcgc cctctggcag cccggcgagg tcggccagcc aacgcagggc ctgattctcg
      961 gccgcggtcg ccccggccgc ctccagccag gaggtaccga agatcgacga tgccgagagg
     1021 gccatatcca tgatctgggc cgcggttgag ggcgggtgcg ccacgaaggc gaggaagcgc
     1081 gggttgtcga ccgccaggca gttaggcgcg atcacgtcgg cgaagaggtc gaaggccgcg
     1141 gcaggtccga tcccggtcgt cgtgatcgcc ccgccgagcg acccggaaag tgcggcggga
     1201 tcgggcggcg attgcagcga ctcatgctga tgagaaatcc gagtctcggc gtgttgaact
     1261 gcccgctcca gtagctgtcg catctcgtcg tcgaaggcaa acaggtcgct catcgatcct
     1321 ccgccgctca tgtccagacg ggacagtagc cacaacaacc aatccagaga tttccggcca
     1381 accggcgctt gacccaaatc gtaatgtggt catacgcgaa ccatacaggg gtgcgaatgg
     1441 ttcgcgcgtt ggaacgggac gagctctgcc cgatcctggc cccagtacct cacctcgaaa
     1501 ggacggtcaa ggatggaccg aacgttcgat gacatcttcg acgacattga agccttcgta
     1561 gaggagcgac ccgtcaccgc ccgcctgatg gtcggtacgg tcggggcgac cgcggtcatg
     1621 atgatcgcgg cgatcctctg gtcgacgacc gacgcatcgg cgagcacgat gatcattgcc
     1681 gcgatcggca tcacgatcgc ctcgtggctg atcgtatcga cggcgctgcc gatcgactgc
     1741 aagatcggac cgatgcatac ccggtcgctc atctgccggc tgcaagcaca gcggcgactg
     1801 gtcgatgtag ttgcggtcgc actgacggtg gtcgtgctgc tgaacatcat cacgacgcaa
     1861 gtctgatcca ggccgcttga tccagatccg atagtcgctc ctcccgcctc gcctggtcgg
     1921 tgtgtgcccg atgggcgcag ggcgtggcgt ggcctgtccg tggctggctg cgctcattga
     1981 taattgcccc gctatcggcc agacgtgatg gatggggcaa tgatttcagt gggtcggttg
     2041 gacgcggcag tcgagcttgt tgatcgcttc atcggggaag gcgtcccggg tgccgggctg
     2101 gtcgtcgccg agcatggcga gatcgtcggc gagtactacg ccggcatggc gcgaccggac
     2161 cgcccggccg atgccgagac gctctggccg ctggcctcga tttccaaact gtacgcggcg
     2221 gccggctgca tggccgcgat cgaggaaggt cggatcgcgc tcagcaccaa ggcctcgcag
     2281 atcttcccgg agttcaggca gggcgggcgc gacaagatca cgcttcgtca tctcctgacc
     2341 cacacctcgg gtctgcccta tgaatcggac cagatggccg agcgcctggc cgcgaagatc
     2401 tcgctcgacg agatggtgcg ggagtcgtac gagggtgatc tgctcttcgc gccgggtacc
     2461 gatcagtcct atagcgacta tgggatcggc ctcgccgggc tgatgtgcgc gaagtcgatg
     2521 gacctcacct tcccggactt ggtccgcagc cgcgtgttcg atcccggtca tctgctcgac
     2581 acctacatgc caccgccgga gaccgaatat caccggatcg cccacatcgt cgggccgctg
     2641 gccgagaaga ccgacggcgc gatgtacaac tcggcccatg cccgcaacct cgcccatccc
     2701 gccttcggga ccatcgccag cgcgcgcgat ctgctccagt tcgggctact gttcgacccg
     2761 aatgggagcc ggtcgctttt ctcgcgcgtt gcgaccacga cgatgatcac cgaccagacc
     2821 ggctacgact tccccgacgc ggtgacggcg ccgatgtcgc cggccgtgcg cgcctggggt
     2881 ctcgggttca tggtcaaggg gcggatgggc ttcccggagc tctgctcgcc ggcggccttc
     2941 ggtcatcccg gcgcgaccgg ctgtatggtg ctggtcgacc cgacctacga cgtcgcgttc
     3001 gccttcgtct cgaaccggca cctcaatacc ggctacgatg aatggcacga tcgtttgatg
     3061 atgatcacga acgtcgtgat ggctggtatg acccgaggat aacgccgctg aaccggatgc
     3121 cgcctgcccc gagaccggga cctgagtccg acctgctgct gtcgcaagta atccggcaga
     3181 tgcggcgatt cggtctgggt gagcgtgtcc tcaagacggc gctggcggcc ggactctcct
     3241 ggcagctggc caccctgatt cccggtaacg atcatccgta tctcgccccg ctcagcgccg
     3301 ttcttctgat gcaactgacg atcgcgcaat cggtcgacct ggccattcag cgcaatatcg
     3361 ggatcgcgat cggcgtgatc gtggccgtcg cgtccttcgc gctcttcggg gtgcatgcct
     3421 ggtcggtcag cctggtcgtg ctgatttccc tggcggccgg aatccagctt cggctcgggc
     3481 agcaagcggt gcaacaggtt gccgtctcgg cgctgattgt gcttctggcc gggagcgtga
     3541 ccggcacgtt cgagtacgcg ttccggcgca tcctcgattc catcatcggc gcgtccgtgg
     3601 cgctggtgct gaactggctg gtcgtcccgc cgatccacat cgccccggcc agcaaggcga
     3661 tcgagcgaat ggcgcaggat ctgtcgctgg cgcttgccga ccttgccgtc agccttcgct
     3721 cgggcatgac ccgcacgcgc gcggaggccc atctcaagag cgcccgggcc ctggcgacgt
     3781 cgttggctgc tgccaacgtg gcgttagggc aggcagagca gagcctgcgg tacaaccggt
     3841 tcgcgcgcgg ccgccacgac gagatggccc gcctccagaa cgccagccgc gcgctcgagc
     3901 acggcgcgat ccagacccgg gtccttgcgc gctcgatggt cacggcgttc gaagaggatg
     3961 cggccgactg gattcagccg gatcagttcg ggcgatcact ggccgatctg cttggccgca
     4021 atgccgcgct cgtccgatat gtcggcggcc ggggcggcat tcgaccggag cttgcgatca
     4081 cgacggagct ccacgaatcc atgctcgcct actggcaggc gcgagccgac ccgggctggc
     4141 tctacgccgg cgaggtcctc gcgatgaccg agcggatggc caaggagttg aacgccgcga
     4201 tcgatgctgg tgacacccga ccttgagcac gctcaatcgc cgcacccagc gccagctcca
     4261 agccctgaca gcacagctca ccgcgaccga agggccgctg cgccgagccc tggacgagct
     4321 cagccggctc ggcatcggcg agcggatcgt caagagcgcg ctggccgcga cgatcgcctg
     4381 gatggtcgcc gcccagctcc cgcgcgagaa tgcgccattc gtcgccgccc tgaccgccgt
     4441 ctacacgatc gacctgacca tcctgaagtc gatccgctcg gcctggcagc ggttggccgg
     4501 gatcggactc ggcatcgcga tggccttcct ggccgcggag ttcctcggcg tgcacgcctg
     4561 gtcggtcggg ctggtgatcc tgctctcgct ggtgatcgga ctgcgcctga atctgaaatc
     4621 ggacggcatg gtccaggtcg cgggcacggc gatcgtcgtg atggtcgtgc gctcgacgac
     4681 cgaggagcgg agcatctacg cgctgatctt cctcgccgat accctgatcg gcacggcgat
     4741 cgggctggca gtcaacagca tcttcgcgcc gccggttttc gtccccgcgg cctggcgggc
     4801 cgtcacggcg ctcggcaatc ggctgatcga cctgatggac cagctcggca cggtggtcgt
     4861 gg
//
LOCUS       DATAOZ010000507         3237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3620716, whole genome shotgun sequence.
ACCESSION   DATAOZ010000507 DATAOZ010000000
VERSION     DATAOZ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3620716"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VHR64_12955"
     CDS             complement(<1..865)
                     /locus_tag="VHR64_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303786.1"
                     /translation="MTTFALMPWRRVSAQGGLWNLFAWFGRACDQAAGILLSPIIVAV
                     FARSIQHDPYRLSWIVVAFGLGWLIGAAIAPLLQQVTVRIMPWIVGGYIVRTAAVVLM
                     TFAVTDDQSSQSQRFQSILICYVAYAVATGISRTAQARHVIVGAPHNLWDARSLPATL
                     GSAAVTGIAAIAVWSALSSQDLSWSTSFGRIWILASVALGVATLAAIREGIEMPELPI
                     ESHRRQSRPDAHGHATLTGASAFVVAGLAALTFVEVVAFLLLFDEFRRQSIYLRGGMA
                     FFVAGWVIARLI"
     gene            complement(930..2120)
                     /locus_tag="VHR64_12960"
     CDS             complement(930..2120)
                     /locus_tag="VHR64_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX3303787.1"
                     /translation="MTQTDIHDALVKSEFDFQGRLGNFLISDLLQMIQLSGKTGTLTL
                     LQGWNNRTITFEKGRITYVASGSRLPSQFDLLVRTGRLTRHQVEAFRQRRPGKTEEEM
                     IDELIARKLLDRTAIERCNEQLLESAIYTLFLWRNCAFTFKAGEVNKTNGVVVSVDGN
                     HLIIEGTRRVDEWIEISPVVPSVFMIFRKRPHLVERTIPEHLRKVYDCVNGDRDVTGI
                     ARATGLSQFDAARALYELAQDRFVESNPPNKLKVVELFKLCVESIYMKMVLFERSRLA
                     LEFEHELNRFASDNGLRVRMAAGRTNRGDMETPLTPVELIELYKTFIGIQNNKLSRLF
                     EPKVFQGLMEGLYLNADPEMQSMMRMYEFFDIDGIAILDMFDNSQPRRQTGTLTPPPT
                     TRAG"
     gene            2403..>3237
                     /locus_tag="VHR64_12965"
     CDS             2403..>3237
                     /locus_tag="VHR64_12965"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303788.1"
                     /translation="MQKTQTMVNATQRAQTRTIPLAMPGMRYAARGGQMLLDGIFAFL
                     AFRIAWQLRYQYEVGGDVAFYDWLPFSQFQNRALLFAGLVVVILLIRGVYWLPRSTGL
                     LDESVMIIGGVTTAMGGVLLTAFLTRFVPSRLVFIFAWAIVIALFVLRRMMSRTMRTA
                     LWKRGIYVDRVLVIGSGESGRRIMEAMLNSPGLGYHLVGFVNELPETSDLSVATEHRV
                     HRAQRVGSIDELERLVGQHSVDEVLVALPADELHLVPNIIEKCRRHAVQFKVVPDLLQ
                     LS"
BASE COUNT          636 a          938 c         1023 g          640 t
ORIGIN      
        1 agatcagtcg cgcgataacc cagccggcaa cgaagaaggc cattccgccg cgcagataga
       61 tcgattggcg ccggaactcg tcgaagagca gcagaaaggc gacgacctcg acaaacgtca
      121 gtgctgccag accagcgacc acgaacgcgc tcgctccggt caaggtcgca tgtccatgcg
      181 cgtctgggcg tgattgcctg cgatgcgatt cgataggcag ctccggcatc tcgatgcctt
      241 cgcgaattgc ggccagtgtt gcgaccccga gcgcgaccga agccaggatc cagatccgcc
      301 caaacgatgt gctccagctc aagtcctggc ttgaaagcgc cgaccacacg gcgatcgcgg
      361 caattccggt tacggccgcc gatccaaggg tcgccggcaa ggagcgggcg tcccacaggt
      421 tgtgcggcgc gccgacgatg acgtgcctgg cctgcgccgt gcgagaaatg cccgtcgcaa
      481 ctgcataagc gacataacag attaagatcg actggaagcg ttggctctgg ctgctctggt
      541 cgtcggtgac ggcaaacgtc atcaggacga cggccgcagt ccggacaatg tacccgccga
      601 caatccaggg catgatccgg accgtgacct gctgaaggag aggtgcgatt gcggcgccga
      661 tcaaccagcc gaggccaaag gcgaccacga tccagctcag gcggtatggg tcatgctgaa
      721 tagaccgcgc gaagacggcg acgatgatcg ggctcaggag gatgccagcg gcctgatcgc
      781 aggcgcgacc gaaccatgcg aaaaggttcc agaggccgcc ttgcgcggaa acccggcgcc
      841 agggcataag ggcaaaggtg gtcacgtgtg ggtccgatgc gaacacgaag gctgcgtcat
      901 gccgctcgat tatagcgagt cggacggttc tagcccgcgc gcgtggtcgg tgggggtgtc
      961 agggtaccgg tctgacggcg tggctgcgag ttatcgaaca tgtcgaggat agcgatgccg
     1021 tcgatgtcga agaactcgta catgcgcatc atcgactgca tctcaggatc ggcattgaga
     1081 tagagccctt ccatcaggcc ctggaagacc ttgggctcga agaggcggga caacttgttg
     1141 ttctggatcc cgatgaaggt cttgtagagc tcgatcaatt ctactggtgt gagcggagtt
     1201 tccatgtcgc cgcgattggt gcgaccggcg gccattcgaa cccgcagacc attgtcggat
     1261 gcgaaccggt tcaattcgtg ctcgaactcg agggcgaggc ggctgcgctc gaagagcacc
     1321 attttcatgt agatcgactc gacacagagc ttgaacagct cgacgacttt gagcttgttc
     1381 ggcggattcg actcgacgaa tcgatcctgg gccagctcgt aaagcgcgcg ggccgcatcg
     1441 aactgcgaga gcccggtcgc gcgggcgata ccggtcacgt cgcgatcacc gtttacgcag
     1501 tcgtagacct tgcgcaaatg ttcggggatc gttcgctcga cgaggtgggg acgttttcgg
     1561 aagatcatga aaaccgacgg gacgacaggg ctgatctcga tccactcgtc gacccggcgg
     1621 gttccttcga tgatcagatg attgccgtcg accgaaacga cgacaccgtt ggtcttgttg
     1681 acctcacctg ctttgaaggt aaaggcgcaa ttccgccaga ggaagagggt atagatcgca
     1741 gactcgagga gctgctcatt gcaccgctca atcgcggttc gatcgagcaa tttccgcgcg
     1801 atcagctcat cgatcatctc ttcttcggtc ttgcctggac ggcgttgccg gaacgcctca
     1861 acctggtgcc gggtcaggcg tccggtgcgc accagaagat cgaactggct gggcaggcga
     1921 gagccggagg cgacgtaggt aatccggcct ttttcgaagg tgatcgtccg gttgttccag
     1981 ccctggagca gcgtcagtgt gccggtcttg ccggagagct gaatcatctg caggaggtcc
     2041 gagatcagga agttgccaag ccgaccctgg aaatcaaact cggacttcac gagcgcatcg
     2101 tggatgtcag tttgtgtcat tccgaatcgg ctcctttggg acggaaggac ccaacgtccc
     2161 tgcattctgt agcgtggaat gcggtctgga tactgccaga gagtcgggtt gcggccaccc
     2221 aaactgccta ccctatcagg acctaggtat tgcctatccg gtgacactgg ggaacgggca
     2281 caaggagcct ggatttagcc ttggatactc cggctccgtg ctatgattcg cgactgaggt
     2341 tcgctttccc ctcttccacg tcggacggtg ttttggtcac gacgaaaggc tggatgcctc
     2401 gcgtgcaaaa aacccaaacg atggtgaacg ccacgcaacg ggcccagaca cgaaccattc
     2461 cattggcgat gcccggaatg cggtacgccg cgcgcggcgg gcagatgctg ttggatggca
     2521 tcttcgcctt tctggcgttc cgcatcgcct ggcagctcag gtatcaatac gaggttggcg
     2581 gcgacgttgc gttttacgac tggctgccgt tttcgcagtt ccagaatcga gcgctgcttt
     2641 tcgccggcct ggtcgtggtc atcctgttga ttcgcggggt ctactggctg ccgagatcga
     2701 ccggcctgct cgacgagagc gtcatgatca ttggcggcgt cacgaccgca atgggcggtg
     2761 tcctgctgac cgccttcctg acccggttcg tgccgtcacg actcgtcttc atcttcgcct
     2821 gggcgatcgt gattgcgctc ttcgtactgc ggcggatgat gtcgcggaca atgcggaccg
     2881 cgctctggaa gcgcggcatc tatgttgatc gcgtcctcgt gatcggatcg ggagaatccg
     2941 gccggcggat catggaggcg atgctcaatt cgcccggact tggatatcac ctggttgggt
     3001 tcgtcaacga gctacctgag acgtccgatc tctcggtcgc gacggagcat cgcgtgcaca
     3061 gggcgcagcg cgtcgggtcg atcgacgagc tggagcgcct ggttggtcaa cactcggtcg
     3121 acgaagtact cgtcgccctc cccgccgacg agctgcatct ggtgccgaat atcatcgaga
     3181 aatgccggcg ccacgccgtt cagttcaaag tcgtgccgga tctcctccag ctctcgc
//
LOCUS       DATAOZ010000508         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_989024, whole genome shotgun sequence.
ACCESSION   DATAOZ010000508 DATAOZ010000000
VERSION     DATAOZ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989024"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2429)
                     /locus_tag="VHR64_12970"
     CDS             complement(<1..2429)
                     /locus_tag="VHR64_12970"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HEX3303789.1"
                     /translation="MRWLNALTAVFLLAGLLANFVVFAPAAPVAAQQKDNTPPPYPTP
                     QSVVAVGSFQTAVGCGQDFDKECDITQLQPNNDGTWTGTFQIPAGSYTYRIVTRSDID
                     RSFGKDGNPDGDDISLDVPDGALGVFFSFNQWTGEIYAVPVNNQVELSTDLGQFPMQP
                     DKDGGFFAVVDGQPNGSFGAQVILDGNPVGDIITVDTGDSGRAVVKMDKDGNLSSDSV
                     EPATLTVNKTDADGNSLPGACFTVYDGKDVAGQGCDASDGEDGATRIEFPVGVGNGLE
                     LRETRTPDNQERADSQDIDINPGDNQVTVTAAGGGGEVTTPEEETPTQVEEETPTEEV
                     TPELGAMTLYTVDENGNPVPNVCYTVSTYSDIQCDEDGDGDMGLTDVPAGDYTAQQAS
                     VPDGYQLDPQEQSITVNGGEEATATFNSPSTQAQTHTVTVTVTDQDGNSVPGACFTVT
                     DANGNQGGEQCDDDQNGDVQLALPNGDYTLSMTTTPDGYQTAEDQSFTVADQDLELPI
                     TVSVAETPTSEVSAGGAINVTVTDSDGFAIIGACVQISGPNSGEVCDNGDGDANGEDG
                     LIRIENLPDGDYTVTVSQLPQGFEPAQPVNVTISGGQEVEAALVSGAAATAEPTSTEA
                     PTTGSLLIRKYDPNKESLSGACFTLTDSNGNATDVCDNQSGDADDRDGRIRVDNLTPG
                     DYTVTESQAPDGYTAAEPKTITISAGETARLNFTNTLAQGTVSISTTDGTNAIGGACY
                     ELSGIGQQCDDDGDGTVQFDAVPPGDYTVTQSSVPQGYSPSDPVDQPVTVTAGETAQV
                     QYVVSVAQASLL"
BASE COUNT          437 a          922 c          967 g          561 t
ORIGIN      
        1 agcagcgagg cctgggcgac ggagacgacg tactggacct gtgcggtctc accagcagtg
       61 acggtcaccg gctggtcgac cggatcggac ggcgagtacc cctgcgggac gcttgattgg
      121 gtgacggtgt aatcgccggg cggaaccgcg tcgaactgga cagtgccgtc gccgtcgtca
      181 tcgcactgct ggccgatacc actcagctca tagcaggctc cgccgatcgc attcgtgcca
      241 tcggtcgtcg agatcgagac cgtgccctgg gccagggtgt tcgtgaagtt caaccgggcg
      301 gtttcgccgg cgctgatcgt gatcgtcttc ggctccgcgg ccgtatagcc atccggcgcc
      361 tgggattcgg tcacggtgta gtcgccaggg gtcaggttat cgacccggat tcgaccatcg
      421 cgatcgtccg cgtcgccgct ctggttgtcg cagacatcgg tcgcgttgcc gttgctatcg
      481 gtcagcgtga aacacgcgcc ggagagcgat tccttgtttg gatcgtactt gcggatcagg
      541 aggctgccgg tcgtcggcgc ttcggtcgag gtcggctccg cagtcgcggc cgcgccgctg
      601 accagcgccg cctcgacttc ctggccgccg ctgatcgtga cattgaccgg ctgggcgggc
      661 tcgaagccct gcggcagctg gctgacggtg acggtgtagt cgccgtccgg caggttctcg
      721 atccggatca gcccgtcttc gccgttcgcg tcgccgtcgc cgttgtcgca gacctcgccc
      781 gagttcgggc cgctgatctg gacgcaggcg ccgatgatcg cgaagccgtc ggagtcagtc
      841 acagtcacgt tgatcgcgcc gcccgcggac acctcggatg tcggtgtctc ggcgacggac
      901 acggtgatcg gcaactcaag atcctggtca gcgacggtga agctctggtc ctcggcggtc
      961 tggtatccat ccggggtcgt cgtcatcgag agggtgtaat cgccgttcgg gagcgcgagt
     1021 tggacgtcgc cgttctggtc gtcgtcgcac tgctcgccgc cctggttgcc gttggcgtcg
     1081 gtaaccgtga agcaggcgcc cggcacggaa ttgccgtcct ggtcggtcac agtgacggtc
     1141 accgtgtggg tctgagcctg ggtcgacggg ctgttgaagg tcgccgtcgc ctcttcgccg
     1201 ccgttgaccg tgattgactg ctcctgcgga tcgagctgat atccgtccgg caccgacgcc
     1261 tgctgcgcgg tgtagtcacc ggccgggaca tcggtcagac ccatgtcgcc gtcgccgtct
     1321 tcgtcgcact ggatgtcgct ataggtcgag accgtgtaac aaacattggg gaccgggtta
     1381 ccgttctcat cgacggtgta gagggtcatc gctcccagtt cgggcgtgac ctcctcggtc
     1441 ggagtctcct cttcgacctg tgtcggcgtc tcttcctccg gcgtggtgac ctcaccgcct
     1501 ccaccagcgg ccgtcacggt cacctggttg tcgcccgggt tgatatcgat gtcctgcgag
     1561 tcggcccgtt cctggttgtc cggcgtgcgg gtttcccgca gctccaggcc gttgccgacc
     1621 ccgaccggga actcgatccg ggtcgcgccg tcttcgccgt cgctggcatc acaaccctgt
     1681 ccggcaacgt ccttcccgtc gtacaccgtg aagcaggcgc cgggcagcga attgccgtcg
     1741 gcgtccgtct tgttgacggt cagggtggcc ggctcgaccg agtcggagct gaggttgccg
     1801 tccttgtcca tcttgaccac ggcccggcca ctgtcgccgg tgtcgaccgt gatgatgtcg
     1861 ccgaccgggt taccgtcgag gatcacttgc gcgccgaacg agccgttcgg ctgcccatcg
     1921 acgacggcga agaagccgcc gtccttgtcc ggctgcatcg ggaactggcc gaggtccgtc
     1981 gagagctcga cctggttgtt gaccggaacc gcgtagatct cgccggtcca ctggttgaac
     2041 gagaagaaga cgccgagcgc gccgtccggc acgtcgagcg agatatcgtc gccatcgggg
     2101 ttgccatctt taccgaacga gcgatcgatg tcggagcggg tcacgatccg gtacgtgtag
     2161 gagccggccg ggatctggaa cgtgcccgtc caggtgccgt cattgttcgg ctggagctgc
     2221 gtgatgtcgc attccttgtc gaagtcctga ccgcagccga cggccgtctg gaaggagccg
     2281 accgccacga cgctctgcgg cgtcgggtag ggcggaggcg tattgtcctt ctgctgggcg
     2341 gcaactggcg cagctggcgc gaagaccacg aagttcgcaa gcaatccggc cagcaaaaac
     2401 acggccgtga gcgcattgag ccaacgcacc tgggatttcc ctgaatgttc gcggtgggtg
     2461 cgagggtgtc tgccaacagc gcgcatcgaa tcgacctccc cagatctgga cggcttccct
     2521 gccgcgtccc acaagagtag tttctgttcg gtcccgaccc ccgggacggc gcaacggcct
     2581 aggcgcggtt gcgttgggga ggtcggagct cccataggcg accgtacgtc agggcgtcat
     2641 catacccgac accccggcgg ccaagtcccg caccaattac ggagacccag ccgttgcaat
     2701 ccccataacg cgatgcaatg aatgtgggtt tcggttgtcg agcggcatcc agggattcaa
     2761 ggcaggtggt ggacccaaga cacggcgtag cgccggcccc cgtagccggc tgggctggca
     2821 acacgcacga tagattgagc aaacgtgctt cggttaccgg gccatacgac gaccaggcgg
     2881 cccagag
//
LOCUS       DATAOZ010000509         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_207776, whole genome shotgun sequence.
ACCESSION   DATAOZ010000509 DATAOZ010000000
VERSION     DATAOZ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207776"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..609)
                     /locus_tag="VHR64_12975"
     CDS             complement(358..609)
                     /locus_tag="VHR64_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303790.1"
                     /translation="MLYSVSATQGRITAIVSEAEREFFVVWTALDRIRAITPPEDLLH
                     LETDRAADTLLRLIREADCIDPSLMLDGTGTTQSLGPNP"
     gene            complement(632..940)
                     /locus_tag="VHR64_12980"
     CDS             complement(632..940)
                     /locus_tag="VHR64_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303791.1"
                     /translation="MNLDEATAALHEVGLAFHNLGDIDQQAVAGSFSTGTHGSGRDLE
                     HLASLLIGGRIVTGDGPVRGMVTPRYELEDAARNCPNRPLRRRYETPGLGVTSGPFVL
                     "
     gene            1047..>1510
                     /locus_tag="VHR64_12985"
     CDS             1047..>1510
                     /locus_tag="VHR64_12985"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX3303792.1"
                     /translation="MFVYVGAYTESPAGTADGISVYRFDPQTGALSLIQVAPGSPNPS
                     FLALAPDRRHLYAVSELDAGGVRSFVRNPVTGELSALNEQSSHGSSPCYISVDPSGRY
                     ALVANYGDGVVTALPIATDGHLQPATGVVQQEGSSVVAGRQNGPHAHMIRATP"
BASE COUNT          279 a          476 c          511 g          244 t
ORIGIN      
        1 ccggaaacgg cgtcgcgcac ggagtaggta atcgttttgg cgccgggatt cgatgcgccg
       61 gagcgaggat ggacgacgac cgtcagcgcg atgaagagaa cggccgcgac gaagaggagg
      121 ctatgccacc agggcacatg cccccaagcc cggtctcgag atcgtgcacg gcgagacgac
      181 gcacgcaaat tcggacgaaa acgctgcatt cagacaacac cgggttaagc gggcgaccaa
      241 cgatcgcgag cagcaccgga gaccaatgct cggcatgtgc tgaatgcgca aagaataggc
      301 gaaagcgcgg ccgattacga ccttgtcacg agcaggtatc gggcgatcgg ggcgggatca
      361 ggggtttggg ccgagggatt gggtcgttcc ggtgccgtcc agcatcagcg acggatcgat
      421 gcaatccgct tcgcggatca atcggagcag ggtatcggcg gcccgatcgg tctccaggtg
      481 aagcagatct tccggcgggg tgatcgcgcg aatccggtct agagccgtcc agaccacgaa
      541 gaactcgcgc tcggcctccg agacgatggc ggtgatgcga ccttgcgtcg ccgagactga
      601 gtacaacatg gactcagcct acggtgtgtg gtcataggac aaaagggcca ctggtcacgc
      661 ccagcccggg agtttcgtac cgcctccgca agggacgatt tgggcaattc ctcgcagcgt
      721 cttccaattc gtagcgcggg gttaccatgc cccgcacggg gccgtcgccg gtcacgattc
      781 gcccgccgat cagcaggctg gcgagatgct caaggtcgcg accggagccg tgggtgccgg
      841 tgctgaagga gccggcgacc gcttgctggt cgatgtcgcc gagattgtga aaggcgagcc
      901 cgacctcatg gagcgcggca gtggcttcgt ccaagttcaa gccactggcc tcccggtatg
      961 cgccggacga cacaccggtt ggttgcaatc ggtaggctgg tgggcgacga tcggcgaatg
     1021 agtcggatta cgggagggat gcgtgcatgt tcgtttacgt cggcgcctac accgagtcgc
     1081 ctgctggtac cgccgatggg atctcggtct accggttcga tccgcagacg ggcgcgctta
     1141 gcctgatcca ggtcgcgccg ggctcgccca acccctcctt ccttgcgctc gctcccgacc
     1201 ggcggcacct ctacgcggtc agcgagctcg acgccggcgg cgtccgctcc ttcgtgcgca
     1261 atccggtcac cggcgagctc tccgcgctca atgagcagtc ctcgcacggc tcctcaccct
     1321 gctacatcag cgtcgaccca tccggccggt acgcgctggt cgccaactac ggcgacggcg
     1381 tggtgaccgc gctcccgatc gcgaccgatg gccacctgca accggcaaca ggcgtcgtcc
     1441 agcaggaggg gtcgagcgtc gtggccgggc ggcaaaatgg gccgcatgcg cacatgatcc
     1501 gggcgacgcc
//
LOCUS       DATAOZ010000510         4174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8096058, whole genome shotgun sequence.
ACCESSION   DATAOZ010000510 DATAOZ010000000
VERSION     DATAOZ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8096058"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2610)
                     /locus_tag="VHR64_12990"
     CDS             complement(<1..2610)
                     /locus_tag="VHR64_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX3303793.1"
                     /translation="MERPVVLPPVLSNKDGAVRWKEGERLHHLMEQACLRFGANDAVV
                     TDHSVMTYRDLDKRANQVARYLIDRGIEPGDRVGLLFDKSVETYVALLAVMKVNAAYV
                     PLDPGFPTERMGFILADASIRSVVSMSSFGARLGAFDVRHVLLDTAKQAIDAKPDTPL
                     AEHEVGPAVDRICYIIYTSGTTGKPKGVVVEHPSICNFVRVASERYGFAPGDRVYQGM
                     TIAFDFSVEEIWVPLMAGATLVPATPGVTLLADELADFLRDRKVTCLCCCPTLLATIE
                     TELPLLRILLVGGEACPHNLVVRWHRAGRTILNSYGPTEATVTATLTELTPDKPVTIG
                     LPLPSYSIVVLDPDADQAAPWGKLGEIGIAGIGLAAGYLNRDDLTQRKFIADFLGVSD
                     NPSKRIYRTGDLGRFNEDGEIEYHGRIDTQVKIRGYRIELIEIEAVLLDLPEIAQAVV
                     TTYEPEPGLVELVAYYSLKQDASVLSRGELTHALRSRLPAYMVPTYLEELPVIPMTLS
                     NKADHRQLPKPQRPRVAAGTKVVVAKTDSEHFLSRALAEVLKVDHVSTEDHFFDDLGA
                     NSLLMARFCARIRKQPGMSTVSMREVYLNPTVAKLAERVSQGGDEGVTTVKPEPFHVP
                     SDVSYYGCGALQLLFYAVCILLGVAFFDAGLEWTYEAVGDPLKVYLRSVTFGVGSFVV
                     LTAVPIAAKWVLIGRWKPESIPIWSLRYFCFWAVKTLTRSAPVVVFSGSPIYNIYLRL
                     LGARIGPNVVIDCRVVPVCTDLIAIGANAILRKDSIVLGYRAQSNFIHTGSVEIGRNA
                     FVGEASVLDIGTAMGDDTQLGHASSLQTGQRVPDGKRYHGSPAQETMSDYCPIDNVPC
                     SGLRRGLFEAIRFL"
     gene            complement(3072..3302)
                     /locus_tag="VHR64_12995"
     CDS             complement(3072..3302)
                     /locus_tag="VHR64_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303794.1"
                     /translation="MTTPTESAMTLTPDPGHDEGQEARQDVAGHDIRRDNDGSIDFDF
                     YRARAAALRNQAIRQAFRHIRFSVVRLQRVET"
     gene            3431..>4174
                     /locus_tag="VHR64_13000"
     CDS             3431..>4174
                     /locus_tag="VHR64_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3303795.1"
                     /translation="MQFLFDDHVLDVDRRELRRAGEPIAIEPQVFDLLVHLVRNRDRV
                     VSKDDLIAAIWHGRIVSESTLTSRINAVRKAVGDSGGAQRLIRTVQRKGIRFIGEVGE
                     VGEPIEPGPAAVTAEAQPPRAPPLRHEIHFCTASDGVRIAYAEVGAGPPLLKAANWLN
                     HLEYDWESPVWSHLLRELAAGHRLIRYDARGNGLSDWEVDDISFEAFARDLESVVEAT
                     GIDRFALLGISQGCAVSIAYAVRYPQRVSH"
BASE COUNT          767 a         1299 c         1370 g          738 t
ORIGIN      
        1 caggaaccgg atcgcctcga acagtccacg ccggagccca gaacagggta cgttgtcgat
       61 cgggcaatag tcggacatcg tctcctgcgc cggagacccg tgataacgct tcccgtccgg
      121 gacgcgctga ccggtctgca aggacgaggc atgtccgagc tgcgtgtcgt cgcccatggc
      181 cgtgccgata tccagcacgc tggcttcgcc gacgaatgca ttgcggccga tctcgaccga
      241 tccggtgtga atgaaattgg attgggcccg gtagcccagc acgatggaat ccttgcgaag
      301 gatcgcgttt gcgccgatgg cgatgaggtc ggtgcagacc gggacaaccc ggcaatcgat
      361 cacgacgttc gggccgatcc tcgcacccag cagacgcaga taaatattgt agatcgggct
      421 gccggaaaac acgaccaccg gcgcactgcg agtcagtgtc ttgaccgccc agaaacagaa
      481 gtagcgcagg ctccaaatcg ggatggactc gggcttccac cttccgatca gcacccactt
      541 cgcggcgatc gggacggcgg tcagcacgac gaaagacccg actccgaatg tgacgctgcg
      601 gaggtagacc ttgagcggat cgcccaccgc ctcataagtc cactcgaggc cggcgtcgaa
      661 gaacgccacg ccgagcagga tgcagacggc atagaacaag agctgaagcg cgccgcatcc
      721 gtaataggag acgtcggagg gaacgtggaa cggctctggc ttgacggtgg tgaccccctc
      781 gtcgcccccc tgagacacgc gctcggcgag cttggcgacg gtagggttca gataaacctc
      841 acgcatcgac accgtcgaca tgcccggttg cttgcggatg cgagcgcaga agcgcgccat
      901 cagcagggag ttcgccccga ggtcgtcgaa gaagtggtcc tcggtggaaa catggtcgac
      961 tttcagcacc tcggccaggg cgcgtgagag gaagtgttcg ctgtccgtct tggcgacaac
     1021 cactttggtg ccggccgcga cgcgcggccg ttggggcttc ggcagctgcc tgtgatcggc
     1081 cttgttgctg agcgtcatcg ggatgaccgg cagttcctct agataggtcg gaaccatgta
     1141 ggccggcagc cggcttcgca gcgcgtgcgt gagctcgccg cgcgacagga ctgatgcatc
     1201 ctgcttgagc gagtaatagg ccacgagctc gaccaggccc ggctcaggct cgtaggtggt
     1261 caccaccgcc tgcgcgatct ccggcaagtc cagcagcacg gcttcgattt cgatgagctc
     1321 gatccggtag ccgcggatct tgacctgggt atcgatccgg ccgtggtatt cgatctcccc
     1381 gtcctcattg aagcggccga ggtcgcccgt ccgatagatc cgcttcgacg ggttgtcgga
     1441 aactcccagg aaatcggcaa tgaacttccg ctgcgtcagg tcatcgcggt tcaagtagcc
     1501 ggccgcgagc ccgatgccgg caatcccgat ctctcctagt ttcccccagg gggcggcctg
     1561 atcagcgtcc ggatcgagaa cgacaatcga atagctcggg agcggcaggc cgatggtcac
     1621 cggtttgtcc ggggtcaatt ccgtcaatgt cgcggtaacg gtagcctcgg tcggcccgta
     1681 cgaattcaag atggtgcgcc cggcgcgatg ccagcggacc accaggttat gggggcaggc
     1741 ctcgccaccg accaacagaa tcctgagcag cggcaactcg gtctcgatcg ttgccagcag
     1801 ggtcgggcag caacagaggc acgtgacctt tcggtctctc aagaaatccg cgagttcgtc
     1861 tgcaagcagg gtgacgccag gagtggccgg gacgagcgtc gcaccggcca tcaacgggac
     1921 ccagatctct tccacggaaa agtcgaaggc aatcgtcatg ccttgataga cacgatcgcc
     1981 aggagcaaac ccgtagcgtt ccgacgcgac ccggacgaag ttgcaaatgc tgggatgctc
     2041 gacgacgacg cctttgggtt tgccggtcgt ccccgaggta tagatgatgt aacagatccg
     2101 gtcgacggcc ggcccaacct cgtgctccgc gaggggcgta tcgggctttg cgtcgatggc
     2161 ctgcttggcg gtgtcgagaa gcacgtggcg cacgtcgaac gcgccgaggc gtgctccgaa
     2221 gctggacatc gatacgacgg atctgatgct cgcgtccgcc aggatgaagc ccatgcgctc
     2281 ggtcgggaaa ccggggtcga gcggcacata ggcggcgttg accttcatca ccgccagcag
     2341 cgcgacatag gtttcaaccg atttgtcgaa cagaagaccg acccggtcgc cgggctcgat
     2401 cccccggtcg atcaggtatc gtgcgacctg gttcgcgcgc ttgtcgaggt cgcgatacgt
     2461 catcacggag tggtcagtaa cgactgcgtc gttcgcaccg aaccgcaggc aggcctgctc
     2521 catgagatga tgaagccgct cgccctcctt ccaacgaacg gcgccgtcct tgttggacaa
     2581 taccggaggt agaacgactg gtcgttccaa gctttcgacc gcggcggcgg gtaggtcgcg
     2641 gacgcttggg aaatgcgcga cgcgggcgag cggggttgcc acgcagcccg acgttagtgc
     2701 ctctcccaag gcgcgctcat gcagcattgt actactctgg acgctattga tccggcgcgc
     2761 acataaagac gcgctcggcg ccacgagccg caggcggccg tagctccaag cgctgggtcg
     2821 gcgtggagat ccctctcgcg acgagcaagt gtgacggtcc ctcgcgcgct cttcatggag
     2881 cgcgtattct tttgccgtta tcagtatgga ggttcctaga tgaatggaag ctgaatgacc
     2941 agaggggccg gtgccgcagt cacctccccc gcaggtcgcc ccctcgcggc cggcaacgga
     3001 gccgccgggg gcgggtacgc cggctgttaa gttgcggcct tgctggcccg gaaagtcgac
     3061 gcggccacgg actatgtttc tacgcgctga agccggacga ctgaaaatcg tatgtgcctg
     3121 aaggcttgcc ggatggcctg gttgcgcaac gcggcggccc gggcgcggta aaagtcaaag
     3181 tcgatggagc cgtcattgtc gcggcggata tcgtgaccgg ccacgtcttg gcgcgcctcc
     3241 tgaccctcgt cgtgaccggg gtctggggtc agggtcatgg cgctctccgt cggcgtggtc
     3301 atgaaatcgc gctcgtcata tgatggaggg tgtcctcctc ccggccggaa ggctttgcgg
     3361 gcgccttgat ttgtcgctga gaatcccccg attattgccg tagaatgctg ccgtccgagg
     3421 tggggccgac ttgcagtttc tctttgatga tcacgtcctc gacgtggatc ggcgcgagtt
     3481 gcggcgcgcc ggcgagccca tcgctatcga gccgcaggtg ttcgacctgc tggtccatct
     3541 ggtgcgcaat cgcgaccgcg tggtcagcaa ggacgacctg atcgcagcga tctggcacgg
     3601 gcgcatcgtg tccgaatcga cgctgaccag ccgcatcaat gccgtgcgca aggccgtcgg
     3661 cgacagcggc ggggcacagc gcctgatccg cacggtgcaa cgcaagggca tccgcttcat
     3721 cggcgaggtc ggggaggtcg gggagccgat cgagccgggc ccggccgctg tcacggctga
     3781 agcgcagccg ccacgggcgc ctcccctgcg gcatgaaatt catttctgca ccgcgtcgga
     3841 cggcgtccgc atcgcctatg ccgaagtcgg ggcgggaccg ccactgctca aggcagccaa
     3901 ctggctcaat cacctcgagt acgattggga aagcccggtc tggagccacc tgctgcgcga
     3961 gctcgccgcc gggcatcggc tgatccgcta cgacgcgcgc ggcaacggcc tctccgactg
     4021 ggaggtggac gacatctcgt tcgaggcatt cgcgcgcgac ctcgagagcg tggtcgaagc
     4081 caccggcatc gaccggttcg cgctgctggg catatcccag ggttgcgcgg tttccatcgc
     4141 ctacgccgtg cgatatccgc aacgtgtgag ccat
//
LOCUS       DATAOZ010000511         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4411467, whole genome shotgun sequence.
ACCESSION   DATAOZ010000511 DATAOZ010000000
VERSION     DATAOZ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4411467"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VHR64_13005"
     CDS             <1..904
                     /locus_tag="VHR64_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX3303796.1"
                     /translation="PEAVIDVLRLLPAETPPMDALRTGLSALGAIDSERFDETPDLDE
                     AIALQARFPGILAAFFRLRSGEQPVKPKPGHNTAQSYLYQLFGKEPEARHWKPLDTYL
                     VLLADHGMNASTFTARVIASTQSDLISSLTGAVGALKGPLHGGAPSKVLDMLHAIGDA
                     DNVEKWLTNALDNGDRLMGFGHRVYKAEDPRAEILRDMAETASDPAFFKLSKLTEDRA
                     LALLNERKPGRRLYTNVEFYSAAVLAAVGLPGDMFTPTFAVSRSVGWSALVLEQVSNN
                     RLIRPQSQFVGPTDKKFTAIESRR"
     gene            1226..1750
                     /locus_tag="VHR64_13010"
     CDS             1226..1750
                     /locus_tag="VHR64_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX3303797.1"
                     /translation="MYRGVSLSALAAVVCWAAMAPFAKYAFDSFSADAYLGLRPIIAT
                     VAAFAVLALRGVPLRINRAAWPRLLLCGAIGYGIAQIGFVKGLDLTSVSHHSVLVSTS
                     PLIAALIMPILHRTRPHGHGLAGMLLGFAGVAMLVGGGGTAGSSIGGDLFVLLSAAVW
                     AGSPVRSMILFAPC"
     gene            1744..1953
                     /locus_tag="VHR64_13015"
     CDS             1744..1953
                     /locus_tag="VHR64_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303798.1"
                     /translation="MLTPDQLDAVNLFCEGHSVHEMADRLFRDPNTIYDRLARARQRL
                     GVTSNHQLVAKAISLRSNGGGPSVA"
BASE COUNT          331 a          703 c          671 g          394 t
ORIGIN      
        1 gccggaggcg gtgatcgatg tcctgcgcct gcttccggcg gagacgccac cgatggacgc
       61 gctgcggacc ggcctttcgg cgctcggcgc gatcgactcg gagcggttcg atgagactcc
      121 ggacctcgac gaagcgatcg cactccaggc ccgcttcccg ggcatcctgg ccgccttctt
      181 ccgcctccgc agcggcgagc agccggtcaa gccgaagccg gggcacaaca ccgcccaaag
      241 ctacctctat cagctcttcg gcaaggagcc ggaggcgcgt cactggaagc cgctcgacac
      301 gtatctcgtc ctgctggccg accatggcat gaacgcctcg accttcaccg ctcgcgtgat
      361 tgcctcgacg cagtccgatc tgatttcgtc tttgacgggt gcggtcggcg cgctcaaggg
      421 cccgctccac ggtggggcgc cgtccaaggt gctcgacatg ctccacgcga tcggcgatgc
      481 cgacaatgtg gagaaatggc ttaccaacgc gctcgacaat ggcgatcgcc tgatgggctt
      541 cggtcaccgc gtctacaagg ccgaggatcc gcgcgccgag atcctgcgcg acatggcgga
      601 gacggccagc gacccagcct tcttcaagct ttcgaagctg accgaggatc gcgccctggc
      661 gctgctcaac gagcggaagc cgggtcggcg cctctacacg aacgtcgagt tctactcagc
      721 ggccgtgctg gcggcggtcg gattgccggg cgacatgttc acgccgacgt ttgcggtctc
      781 ccgctcggtc ggctggagcg cgcttgtgct ggagcaggtc agcaacaacc ggctgatccg
      841 cccgcagtcc cagttcgtcg gcccaaccga caagaaattc accgcgatcg agagccgccg
      901 ctaatccatg cgaatccaat tagggccgcc cggactgccg ggcggcccta attgcgtagc
      961 accggcccac cgtggccggc ttggtgtcca tggcgcgtaa cgtcgatgga gcaaggccgg
     1021 catcatcgac cctcccttcc gaagacgaag cggcccagag gcgggccgcg ctacgcccaa
     1081 catttgggca tgtgcgcccg gtgggaccgg gcggtgattc cggatcgatt gcggacgcgg
     1141 tagattttcc gcagcgagct gaattcgcca tcggatcgaa ccgcgtgcat cctgattcat
     1201 cgttctcttc gcggcgagaa ggcacatgta caggggagtg agcctgtccg cgttggcggc
     1261 ggttgtctgc tgggccgcga tggcgccctt cgccaagtac gccttcgact ccttctcggc
     1321 cgatgcctac ctcggcctgc gcccaatcat cgccacagtt gcggcgttcg cagtcctggc
     1381 cctgcgcggc gtcccgttgc gcatcaatcg cgcggcctgg ccccggcttc tgctctgcgg
     1441 cgcgatcggc tatgggatcg cacagatcgg cttcgtcaag ggactggatc tgaccagtgt
     1501 ctcgcaccac tcggtcctgg tctcgacgag tccgctgatc gccgcgctga tcatgccgat
     1561 cctccatcgc actcgcccgc atggtcacgg gttggccgga atgctcctcg gattcgccgg
     1621 cgtcgcgatg ctcgtgggcg ggggcgggac ggcgggctcg tcgatcggcg gcgatttgtt
     1681 cgtgctcctg tcggccgcgg tctgggccgg gtccccggtc cggtcgatga tcctcttcgc
     1741 gccatgctga ccccagacca gctcgatgcg gtcaacctct tctgcgaggg acactcggtc
     1801 cacgagatgg cagatcgcct gttccgcgat cccaacacga tctacgaccg actggcccgt
     1861 gcccggcagc ggctcggcgt gacatcaaat caccagctgg tggccaaagc catctcgctc
     1921 cggtcgaacg gcggcggtcc gtcggtagca taagttgcat ctgcgtgaaa tacgggattt
     1981 tcccgtattg ccttccggta catttctcct atgattcgga tactggttgt gaattgaacg
     2041 atccatgccg agggttgcct cgccgggcga tgacgagacg tcaggagctg gcgagtcgg
//
LOCUS       DATAOZ010000512         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_991542, whole genome shotgun sequence.
ACCESSION   DATAOZ010000512 DATAOZ010000000
VERSION     DATAOZ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991542"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1642)
                     /locus_tag="VHR64_13020"
     CDS             complement(53..1642)
                     /locus_tag="VHR64_13020"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3303799.1"
                     /translation="MASVVNQPAKYDAIVVGAGHNGLVAACYLAGAGLNVCVLERYHE
                     VGGATISEETIPGFKLSTGSYVLSLAPRKIFDELGAWEGIELLERNPRFFAPFPDGSS
                     LTYWNDHEKWLESVRKVNPKDAEAYDVYDNAVEVACSVMDKYILRRPPSWDEVASNFR
                     TPEEQKIFQMFYLGAVSDIAEYYFDSDQMQATVSSSGIIGTFRGPRDPGTGYVKLYHS
                     MGMATGHRGRWAYVRGAMGAITQALRKIAERRGVTIRTNANVQQIVVRNGRAAGVLLD
                     SGEELTSDIVLSNADPKRTYLSLIEERDLPADYRRSISNIKIESPVIKINMAVRELPK
                     FLALPDDQQLQGSTGGLFIAPSIDYIQKAYYDAKSGKPAEHPFMNIHMQSAVDRTVAP
                     GDMHTISIFTQYFPYTLAEGTWDERRDEVAQNVIREFAKFAPNIPDAIVGMQVLTPPD
                     IEARFGMTGGHIFHGELVPEQAFDMRPVPGSSSYEGPIGGLFLCGSGAWPGGCVMGAP
                     GHNAAQEIIGRLQEGRVLKGA"
     gene            complement(1787..1969)
                     /gene="rpsU"
                     /locus_tag="VHR64_13025"
     CDS             complement(1787..1969)
                     /gene="rpsU"
                     /locus_tag="VHR64_13025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX3303800.1"
                     /translation="MRVEIKDSESFDQLLRRFQKGIERSGIIREHRRGLRFISTQEEN
                     RAKRRKAERRRRRNQA"
     gene            2089..3186
                     /locus_tag="VHR64_13030"
     CDS             2089..3186
                     /locus_tag="VHR64_13030"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX3303801.1"
                     /translation="MSTSRSVSGLAVTPGRPHSLHLTEIELPLTGPNDVRIKVLEAGI
                     CGTDREIIEAHFGSAPAGSTSLVIGHEVLGEIEAVGDKVTAIAPGDLVTTTVRRPCGC
                     PQCAAGASDFCVDMRFTERGIVGRHGFMTDRFVESAEYVVPVPADLREIGVLIEPTSV
                     AEKAWRVAAGVQSRIAAWTPQTAVVYGAGPIGLLATLVLRAKDIDVYTIARRPANETP
                     ASAIVEACGASYLSSREQDVNSIKARLPNVDLIIECSGSTEAVESAIKLLGINGVLVL
                     LSLTGGSRSATLSLDKINFDFVTGNKTMVGSVNSTDADFRAAIADLQEIERRWPGLAA
                     RLITHRLTSLDEAVGLMESTRGAIKAIVELD"
BASE COUNT          578 a         1041 c         1039 g          620 t
ORIGIN      
        1 acgccgcagg tgccctccat atcggctgag atcagtacct tcatcgacgg acctatgctc
       61 ccttcagtac ccggccttcc tgcagccggc cgataatctc ttgcgccgcg ttatgtccag
      121 gcgcgcccat cacgcagccg cccggccagg cgccagagcc gcagaggaac aggccgccga
      181 tcggcccctc gtacgacgag ctacccggca ccggccgcat gtcgaaggcc tgctcgggca
      241 ccagctcgcc gtggaagata tggccgccgg tcatgccgaa gcgcgcttcg atatccggcg
      301 gcgtcagaac ctgcatgccg acgatcgcgt ccgggatgtt cggcgcgaac ttcgcgaact
      361 ccctgatcac gttctgcgcg acctcatccc tccgctcatc ccacgtcccc tcggcaaggg
      421 tgtatgggaa gtactgcgtg aagatcgaga ttgtgtgcat gtcgcccggc gcaaccgtgc
      481 ggtccacggc ggactgcatg tggatgttca tgaacgggtg ctcggcaggc ttgcccgact
      541 tcgcgtcgta atacgccttc tggatgtaat cgatgctcgg cgcgatgaag aggccgccgg
      601 tgctgccctg gagctgctga tcatccggca gcgccaggaa tttcggcagc tcgcgcacgg
      661 ccatattgat cttgatgacc gggctctcga tcttgatatt gctgatcgag cggcggtaat
      721 cggccggcaa gtcgcgctcc tcaatcagcg acaggtaggt gcgcttggga tcggcgttcg
      781 agaggacaat gtccgacgtc agctcttcgc cggaatcgag cagcacgccc gccgcgcggc
      841 cattcctgac cacgatctgc tggacgttgg cgttggtccg gatcgtcaca ccgcgccgct
      901 cggcgatctt cctgagtgcc tgcgtgatcg cacccattgc cccgcgcacg taggcccagc
      961 ggccgcgatg accggtcgcc atgcccatcg agtggtagag cttgacgtag ccggtaccgg
     1021 gatcgcgcgg gccacggaag gtgccgatga tgccgctcga gcttacggtg gcctgcatct
     1081 ggtcgctatc gaagtagtac tcggcgatgt cggatacggc gccgaggtag aacatctgga
     1141 agatcttctg ctcctcgggc gtccggaagt tgctggcgac ctcgtcccag ctcggcggcc
     1201 ggcgcagaat gtacttgtcc atcacgctgc aggcgacttc gaccgcgttg tcgtaaacgt
     1261 cgtatgcctc agcatccttc gggttgacct tgcggacgct ctccagccac ttctcgtggt
     1321 cattccagta ggtcagcgag ctgccatccg ggaagggcgc gaagaagcgc gggttgcgct
     1381 ctagcagctc gatgccttcc cacgcgccga gctcgtcgaa gatcttgcgt ggggccagcg
     1441 agaggacata ggagccggtc gagagcttga aacctgggat cgtctcctcg gagatcgtcg
     1501 cgccgccgac ctcgtggtag cgctcgagca cgcagacgtt gaggccggcg ccggccagat
     1561 agcaggcggc tacgagcccg ttatgacccg caccgacgac gatcgcgtcg tacttcgcgg
     1621 gctgattgac aaccgacgcc atgggatggt cctccgagtc gctgtgcgac ggtgagcagg
     1681 ttgagtcgca aatgccccgg taggatagca tcggtcccga aacgcaaatg agccggactg
     1741 catgtccggc tcatcgcatt cagattcgag agatgtgggg tatcggtcag gcctggttcc
     1801 ggcggcggcg gcgctcggcc ttccgtcgct tggcgcgatt ctcttcctgc gtgctgatga
     1861 aacgcagccc gcggcgatgc tcgcggataa tcccggaccg ttcgatgcct ttctggaatc
     1921 gtcgaagcag ttgatcgaac gactccgaat ccttgatctc aacccgcatg cgtaaaattc
     1981 accacctgaa ttggtatggg gcgtccgtga cgcgcaccaa agatccgtcc gatcattgtc
     2041 ggcctcgacc gagtatacga catcggtcaa tacaacggga gtaattacgt gtccaccagc
     2101 agatcggttt caggcctggc cgtgaccccg ggtaggccgc attcgctcca cctgaccgag
     2161 atcgaactgc cactgacagg cccgaacgac gtgcgaatca aggtccttga ggcaggcatt
     2221 tgcgggacgg accgcgagat catcgaagcg cattttggct ccgcgccggc tggttcgacc
     2281 agcctggtga tcggccacga ggtcctcggc gagatcgagg cggttggcga caaggttact
     2341 gccatcgcgc cgggtgatct ggttacgacc accgtgcgcc ggccctgtgg ctgcccgcaa
     2401 tgtgcggccg gcgcctccga cttctgcgta gacatgcgat tcaccgaacg tggaatcgtc
     2461 ggccggcacg gcttcatgac cgaccgcttc gtcgagtcgg ccgagtacgt cgtgccggtc
     2521 ccggccgatt tgcgcgagat cggtgtactg attgagccga ccagcgtggc cgagaaggca
     2581 tggcgagttg cggctggggt gcagtcgcgc atcgccgcat ggacgccgca gacggcggtc
     2641 gtctacggtg ctggcccgat cggactcctc gcgacgctgg tgctccgggc gaaagacatc
     2701 gacgtctaca cgatcgcgcg ccgcccggcg aatgaaaccc cggcctccgc gatcgtcgag
     2761 gcctgcggcg catcctacct ttcgagccgc gaacaggacg tcaattcgat caaggcgcga
     2821 ctgcccaatg tcgacctgat catcgagtgc tccggctcga ccgaagcggt cgaatcggcc
     2881 atcaagctgc tcggcatcaa cggcgtgctt gtcctgctga gcttgaccgg cggctctcgc
     2941 agcgcaacgc tgtcgctcga caagatcaat tttgacttcg tgaccggcaa caagacgatg
     3001 gtcgggtcgg tcaactcaac cgatgccgac ttccgcgccg ccattgccga ccttcaggag
     3061 atcgagcgcc gctggccggg tcttgccgcg cggctgatca cccaccgcct gacctcgctg
     3121 gacgaggcgg tcggattgat ggagtcgacg cgcggggcaa tcaaggccat tgtcgagctc
     3181 gactgacctc taccaccagc cgtacgggat atttcggtgc gggttgagga tagggtaggt
     3241 aggctcagcg ataaacatgt cgatccgccg cagcaggg
//
LOCUS       DATAOZ010000513         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4148660, whole genome shotgun sequence.
ACCESSION   DATAOZ010000513 DATAOZ010000000
VERSION     DATAOZ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4148660"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VHR64_13035"
     CDS             <1..366
                     /locus_tag="VHR64_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin hemolysin A"
                     /protein_id="HEX3303802.1"
                     /translation="GADQIRGGPTGDDTILGSAGADRLYGESGNDVLKGGDDNDVIDG
                     DDGSDQIFGGGGTDKLVGGTGNDLVAGEEGDADRVYGVAGDDTVTGGPGEADYCSGGA
                     GFNVASPDHGCEILSALSP"
     gene            591..>3745
                     /gene="rpoB"
                     /locus_tag="VHR64_13040"
     CDS             591..>3745
                     /gene="rpoB"
                     /locus_tag="VHR64_13040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX3303803.1"
                     /translation="MSVNGKSATILPPVVDVDRQVVSNTGINRRTFSRIPTVLEMPNL
                     VQVQIESFNWFLQEGLKELLEEISPISDHHRKMELYITDPRFEEPWKRLPTEDLRRRA
                     EADPRVAEIYCRDRDLNYAAPLFVSARLVMRETGEIKETGPDGIFLGDFPMMTTDGTF
                     IINGAERVVVSQLVRSPGVYFEVSQDPTTGKNVSTAKLIPNRGAWLEFETSNRDVISV
                     KVDRKRKMPVSILLRAIGLETNEQLLDAFADVDTNEDRRYIQTTMEKETTKSRDEALI
                     ELYRRLRPGDPPTKDNASTMLENLFFNDRRYDLARVGRHKLNQRLHGEKQGTENQIST
                     RILTVEDLVEIIREMIRINNGQGQGDDIDHLGNRRIRAVGELIQMHVRTGFQRLERGI
                     RERMTIQDPETVTPQSLISTTRPVMAAVREFFGGSQLSQFMDQTNPLAELTHKRRLSA
                     LGPGGLSRDRAGFDVRDVHSSHYGRICPIETPEGPNIGLIGSLATYGKINQYGFIETP
                     YRRVVASINLEDPVIDPVSQELRDTAVNPANGKTVLESGTVLTQANVDALKKAGVKLV
                     RIKAVASNIIDYLSADKETNVSIAQASTPLEENGRIIPDQVTVRVGGSGHSFPQIPAE
                     QVAYMDVSPKQVVSVATALIPFLEHDDANRALMGANMQRQAVPLVRPRSPIVGTGVEH
                     QAAKDSGQVLVSRHNGTVKHVNSREVVIVEDDGTEHIYQLRKFVRSNQDTCINQRPSV
                     SKGDRVLAGQIVADSSSTEDGELALGQNVLVAFMPWEGGNFEDAILISERLVRDDVFT
                     SIHIEKYETEARDTKLGPEEITRDIPNVGEESLANLDENGIIRIGAEVRPNDILVGKV
                     TPRGETELSAEERLLRAIFGEKAREVKDTSLRVPHGVHGKIIDVKVFKRDESPDHELP
                     AGVNEMVRVSIAQKRKISEGDKMAGRHGNKGVISRILPIEDMPYLPDGQPVDIILNPI
                     GVPSRMNLGQVLETHLGWAAERLGYKVATPVFDGAREIEIRKALVDAGLPEDGKVDLY
                     DGRTGEKFDRPVTVGIIYMLKL"
BASE COUNT          769 a         1115 c         1223 g          638 t
ORIGIN      
        1 ggcgcggatc agatccgcgg cggtccaacc ggcgacgaca cgatcctggg cagcgctggc
       61 gcagatcggc tctacggcga atccgggaac gatgtcctta agggcggaga cgacaacgac
      121 gtcatcgatg gcgacgacgg atcagatcaa atcttcggtg gaggcggcac ggacaagctg
      181 gtaggtggga ccggcaatga cctggttgcc ggcgaggagg gcgacgccga tcgagtctat
      241 ggtgtggccg gtgatgacac ggtaacgggc gggccgggag aggccgacta ctgctcgggc
      301 ggggcgggat tcaacgtcgc gtcacccgat catggctgcg agattttgag cgcactaagc
      361 ccttgacgaa atgtccgcaa aggcgtatgc tactcgactg ccgctcttcg cggcggtagc
      421 ggcgggtgcg tcgcggggtg acattaccat acgagcatac atcacgaatt gaccacagtc
      481 cgatttaggg aggggagaat ctgccctccc gatttgtgtt ggttaggttt ttgcgaaacc
      541 cacttgggtc aatcggtgcg ccgagtggga cgcgactgga ggaaacgcca atgtccgtga
      601 atggcaagtc ggcaacgatc ctgccgcccg tggtggatgt ggaccgccag gtggtctcga
      661 acacgggtat taatcgccgg accttttcgc ggattccgac cgtgctcgag atgccgaatc
      721 tcgtgcaggt ccagatcgaa tcattcaact ggtttctcca ggaagggctc aaagagctac
      781 tcgaagagat ctccccgatc agcgatcacc atcgcaagat ggagttgtat atcactgatc
      841 cccgattcga ggaaccgtgg aagcggcttc cgaccgaaga tcttcgccgg cgcgccgaag
      901 ccgacccgcg cgttgcggag atctactgtc gtgaccgcga tctgaattat gccgcgccgc
      961 tcttcgtatc ggcccggttg gtcatgcgcg aaaccggtga aatcaaggaa accggtccgg
     1021 acggcatctt cctgggcgac ttcccgatga tgaccacgga cggcaccttc attattaatg
     1081 gagcggagcg cgtcgtcgtc tcgcagctcg tccggtcccc gggcgtctac ttcgaggtct
     1141 cccaagatcc gacgaccggc aagaacgtct caaccgccaa gttgatcccg aaccggggtg
     1201 cctggctgga gttcgagaca tccaaccggg acgtcatctc ggtcaaggtc gatcgcaagc
     1261 gcaagatgcc ggtctcgatc ctgctccgtg ctatcggcct ggagacgaac gagcagcttc
     1321 tggacgcctt cgccgatgtc gataccaatg aggatcgccg gtatatccag acgacgatgg
     1381 agaaggagac gaccaagagc cgcgacgagg ctctgatcga gctctatcgt cgcctacgcc
     1441 cgggcgatcc gccgacgaag gacaacgcct ccacgatgct ggagaacctg ttcttcaacg
     1501 atcgccgcta cgatctggcg cgcgtcggcc ggcacaagct caaccagcgg ctgcatggcg
     1561 agaagcaggg cactgagaat cagatctcga cccgcatcct gaccgtcgag gatctggtcg
     1621 agatcatccg tgagatgatc cggatcaaca atggtcaggg tcagggcgac gacatcgacc
     1681 acctcggcaa ccgtcgcatc cgtgcggtcg gtgagttgat ccagatgcac gtccgcaccg
     1741 ggttccagcg cctcgagcgc ggtatccgtg agcggatgac aatccaggat cccgagacgg
     1801 tcacgccgca gagcctgatc agcacgaccc gccccgtcat ggcggccgtg cgcgagttct
     1861 ttggcggatc gcagctctcg cagttcatgg accagaccaa cccgctggcc gagctgacgc
     1921 acaagcgccg actgtcggct ctcgggccgg gcggtctcag ccgcgatcgc gcgggattcg
     1981 atgtccgtga tgtgcactca tcgcactatg gccggatctg cccgatcgag acgccggaag
     2041 gaccgaacat cggtctgatc ggatcgcttg cgacctacgg caagatcaac cagtacggct
     2101 tcatcgagac gccgtaccgg cgcgtggttg caagcatcaa cctcgaggat ccggttatcg
     2161 acccggtcag ccaggaattg cgcgacaccg cggttaatcc ggcgaacggc aagactgtgc
     2221 tcgaatcggg cacggttctg acccaggcaa atgtcgatgc gttgaagaag gcgggcgtca
     2281 agctcgtccg gatcaaggca gtcgccagca acatcatcga ttacctgtcg gcggacaagg
     2341 agacgaacgt ctcgatcgca caggcatcga caccgctgga ggagaatggg cggatcattc
     2401 cggaccaggt cacggttcgg gtcggcggca gcggtcacag cttcccgcag attccggccg
     2461 agcaggtcgc gtatatggac gtctctccga agcaggtcgt ttcggtggcg acagcgctga
     2521 ttccgttcct cgagcacgac gacgcgaacc gggcattgat gggcgcgaac atgcagcgcc
     2581 aggctgtgcc attggttcgg ccgcggtcgc cgatcgtcgg caccggagtc gagcatcagg
     2641 cggcgaagga ctctgggcag gtgctcgtct cgcgccacaa cggcacggtc aagcacgtca
     2701 actcgcgcga agtcgtgatc gtcgaggacg acggcacgga gcatatctat cagctgcgca
     2761 agttcgtccg ctcgaaccag gatacctgca tcaaccagag accgagcgtc agcaagggcg
     2821 atcgggtgct ggccgggcag attgtcgccg attcgtcgtc gactgaagat ggcgagctcg
     2881 cactcggcca gaacgtcctc gtggcgttca tgccgtggga aggcgggaac ttcgaggacg
     2941 cgatcctgat cagcgaacgg ctggtccggg acgacgtctt cacctcgatc cacatcgaga
     3001 agtacgagac cgaggcgcgg gacaccaagc tcggtccgga agagatcacg cgggatatcc
     3061 cgaatgtcgg cgaggagagc ctggcgaacc tcgacgagaa cggcatcatc cgcatcggcg
     3121 ccgaggtccg gccgaacgac atcctggtcg gcaaggtcac gccgcgcggc gagaccgagc
     3181 tgagcgctga ggagcgactg ctccgcgcga tcttcggaga gaaggcgcgc gaggtgaagg
     3241 acacctcact gcgcgtgccg cacggtgtcc acggcaagat catcgacgtc aaggtcttca
     3301 agcgggacga gagcccggat cacgagctcc cggccggcgt caacgagatg gtgcgcgtca
     3361 gcatcgcgca gaagcgcaag atctcggaag gcgacaagat ggccggtcgc cacggcaaca
     3421 agggcgtgat cagccggatt ctgccgatcg aggacatgcc gtacctgccg gatgggcagc
     3481 cggttgacat catcctcaac ccgatcggtg tgccgtctcg tatgaatctt gggcaggtgc
     3541 tcgagacgca cctcggctgg gcagccgagc ggctcggcta caaggtcgcg acgcccgtct
     3601 tcgacggcgc gcgcgagatc gagatccgca aggcgctggt cgatgccgga ttgccggagg
     3661 acggcaaggt ggatctgtat gacggccgga cgggcgagaa gttcgatcgc ccggtgacgg
     3721 tcggcatcat ctatatgttg aagct
//
LOCUS       DATAOZ010000514         4614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2313113, whole genome shotgun sequence.
ACCESSION   DATAOZ010000514 DATAOZ010000000
VERSION     DATAOZ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313113"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VHR64_13045"
     CDS             <1..382
                     /locus_tag="VHR64_13045"
                     /inference="COORDINATES: protein motif:HMM:NF021484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HEX3303804.1"
                     /translation="VVLDTSGSMDRSLLGKALGAIASYAITHDVPAVRVVFCDAAAHD
                     QGYLEPATIAGRVKVRGRGGTVLQPGVDLLEQAEDFPKDGPLLVITDAWCDKVSVHRE
                     HAFLVPKGARLPFVPRGPVFRVTD"
     gene            723..1538
                     /locus_tag="VHR64_13050"
     CDS             723..1538
                     /locus_tag="VHR64_13050"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX3303805.1"
                     /translation="MTEQHPPSVEALSFAIDASRPTPLYQQLSDIIRRQVLGAQWPAG
                     SAIPSERELMRITGLSRMTVRQAIDSLTREGLLTRVHGRGTFIVPERVEQDMRGVYSF
                     TARIEAQGRTPSTRLLEAVELEADPEDALALRIEAGAPIFRLMRLRCIDGEPAVVDIV
                     KIPGACCPGLLDHDLTESLYAILTERYHVPPLRSTDTIEAIGAPREIAKPLAVRPGAP
                     LILMRRLATTHGDAPIELTEEYARPDRLRYRMRLYADPLVDSGPDDHREVQTR"
     gene            1535..2683
                     /locus_tag="VHR64_13055"
     CDS             1535..2683
                     /locus_tag="VHR64_13055"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3303806.1"
                     /translation="MTRPLKAAVVGTGFIGPVHIEALRRLGIEVTGILASTKEHGQKR
                     ADALRLPKAYGSLDELLAEPGLDAVHITTPNYLHKPMVEAAIAAGKHVVCEKPLAMNS
                     EEGQYLLGLAEKAGIVHATNFNFRFYPLCQEARSLVQGGKLGTIYQIYGRYAQDWLLK
                     ETDWNWRLETDLGGDMRAVADIGSHWLDLTRFIAGQDINEVCADFATFLPVRKRPLVP
                     LETFAGKELQPDEYEEREIRTEDYASILLRFSDGARGVLTVSQVAAGRKNRNEFEING
                     STGSVSWNSERVEEMWLGHRDKSSELLLKDATLMSSEARMTTEVPGGHAEGFRDTFKM
                     LYARVYRAIEAGGPPANPDYPTFADGVYALKLGEAIFESAQKRAWVTV"
     gene            2808..3719
                     /locus_tag="VHR64_13060"
     CDS             2808..3719
                     /locus_tag="VHR64_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3303807.1"
                     /translation="MKLGFFTAAFPGLDLEQVATWAAESNFQTLEIACWPAGKAERRY
                     AGVSHIDVSDLDKSGAKKIRKMLNGLKLDISSLGFYPNPLHPDAEVREAAFDHIKKVI
                     DAAVLLEVPIVGTFIGADQSKSAAENLAAFGLLWPPIIKYAGERGIKIAIENCPMLWH
                     DTWPGGQNVAYSPTIWTKMFEMIPDENFGLNYDPSHLIWQFIDEVKPIREFRDRIFHV
                     HAKDMWLDREKLARDGILSAGVGWAIPRLPGLGETRWGELIAALYANGYDSVISIEHE
                     DRAFEKTEELVKRGFYLTRDYLNPWIV"
     gene            3741..>4614
                     /locus_tag="VHR64_13065"
     CDS             3741..>4614
                     /locus_tag="VHR64_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX3303808.1"
                     /translation="MSDRFAVGVDVGGTNLKFAIVNEAGSVVARLTIPTEGHEGHDHV
                     LQRMIHGVNELRSRGPAGCAIESIGVGVPGMLDMVTGTTIDLPNLAGKWPNVAVKSIM
                     EAALHVPVHMINDVKAFTIAERELGAAKGANNVVCYAIGTGIGGGVLIDGKVHFGIGG
                     AAGELGHIIVNPGGVRCSCGNYGCVEAMASGPAIIGEANRRVVQGFTTRLTEMIGDDL
                     NRMTPGLVEQAAAEGDPAAREVLERAGYFLGLSMAGMIAALAPDVVVIGGGVVKPHGV
                     YWTAFESTARASNTV"
BASE COUNT          828 a         1371 c         1599 g          816 t
ORIGIN      
        1 tgtcgttctg gacacgtcgg gttcgatgga ccggagtctg ttgggaaagg cgctgggcgc
       61 gatcgcaagt tatgcgatca cgcacgacgt tccggctgtt cgcgtggttt tttgtgacgc
      121 ggctgcccat gatcaggggt accttgagcc ggcaaccatt gctggtcgtg tgaaggtacg
      181 cggtcgcggc ggaaccgtac tccagccggg tgtcgattta ctcgaacagg cagaggattt
      241 tccgaaagac gggccgctgc tggttatcac cgacgcctgg tgcgacaagg tgagtgttca
      301 ccgtgaacat gccttcctag tgccaaaggg tgcccggtta ccgtttgtgc cgcgggggcc
      361 agtctttcga gtaacggact aggacgcgtc ggggaggcca gtagtgggag gcggcctcga
      421 tttggagccc ggactccaat ctaatcatgg cttgtacacg gctgatggcc ggatcatcgt
      481 atcaaacgat aaggatcgaa ttcgcgtccc tcccaagcca ggcttgacaa cttggtttcc
      541 ccttcatggc attctccaat atgactttgc ctttcgagac gctagggacc tgctgattac
      601 ggtcggcaac cacctcgggc tgcggccgcc gtgcgcacaa tgctgttgac acgctctgcc
      661 ggggttggat aattccgtat cccgttcact ttggactggt cgccgggctc gtgaggtcga
      721 cgatgactga acaacacccc ccgtctgtgg aagcgctctc attcgcgatc gacgcgagtc
      781 gtccgacccc tctctaccag caactcagcg atatcatccg gcgccaggtc ctcggcgcgc
      841 agtggccggc cggctccgcg atcccctccg aacgcgagtt gatgcggatt accggactga
      901 gccggatgac ggtccggcag gcgattgact ccctgacccg ggaggggctg ctgacccgcg
      961 tccacgggcg cggcacgttc atcgtcccgg agcgggtcga gcaggacatg cgtggggtct
     1021 actcgtttac cgcccgcatc gaggcgcaag ggcgcactcc ttcgacccga ctgctcgagg
     1081 cggtcgagct ggaggcagac ccggaggacg ccctcgcgct ccggatcgag gcgggcgcac
     1141 cgatcttccg gctgatgcgc ctgcgctgca tcgatggcga gccggcagtg gtcgacatcg
     1201 tcaagatccc gggcgcctgc tgccccggcc tgctcgatca cgacctgacc gagtcgctct
     1261 atgccattct tacagagcgg taccatgtgc cgccgctgcg atcgaccgat acgatcgagg
     1321 cgatcggcgc gccgcgggag atcgcgaagc cgctggccgt ccggcccggc gcgccgctga
     1381 tcctgatgcg gcggctggcg acgacgcatg gcgatgcgcc gatcgagttg accgaggagt
     1441 acgcgcggcc ggatcgattg cgctaccgga tgcggctcta cgcggatccg ctggtcgaca
     1501 gcgggccgga tgatcacagg gaggttcaga ccagatgacg cgtccgctca aagccgccgt
     1561 cgtcgggacc gggttcatcg gccccgtgca catcgaggcg ttgcgccggc tcgggatcga
     1621 ggtgaccggc atcctcgcgt cgacaaagga gcacggccag aagcgggccg atgcgctgcg
     1681 cctaccgaag gcgtacggct cgctcgatga gctgctggcc gagccgggtc tggacgcggt
     1741 gcacatcacc acgccgaact acctgcacaa gccgatggtg gaggcggcga tcgcagcggg
     1801 caagcacgtc gtctgcgaga agccactggc gatgaactcc gaggaggggc aatacctgct
     1861 cggtttggcc gagaaggcag ggatcgtcca cgcgaccaat ttcaacttcc gcttctaccc
     1921 gctctgccag gaggcgcggt cgctggtcca gggcgggaag ctgggcacga tctaccagat
     1981 ctatggccgc tacgcgcagg attggctgct gaaggagacc gactggaact ggcgcctgga
     2041 gaccgacctc ggcggcgaca tgcgggccgt ggccgacatc ggctcgcact ggctggatct
     2101 gacccggttc atcgcggggc aggacatcaa cgaggtctgt gccgacttcg cgaccttcct
     2161 gccggtgcgc aaaaggccgc tggtcccgct cgagaccttc gccggcaagg agctccagcc
     2221 ggacgagtat gaggagcgcg agatccggac cgaggactac gccagcatcc tgctccgctt
     2281 ctcggacggc gcgcgcggcg tattgaccgt ttcgcaggtc gcggccgggc gcaagaaccg
     2341 caatgagttc gagatcaacg gcagcaccgg ctcggtttcg tggaactcgg agcgggtcga
     2401 ggagatgtgg ctcggtcacc gggacaagtc gagcgagctg ctgctcaagg acgcgacgct
     2461 gatgtcgagc gaggcgcgaa tgaccaccga ggtaccgggc ggacacgccg agggcttccg
     2521 cgatacgttc aagatgctct atgcgcgcgt ttaccgggcg atcgaggcgg gcggtccgcc
     2581 ggcgaacccg gattatccga ccttcgctga cggtgtctac gcgctgaagc tgggcgaggc
     2641 gatcttcgag agcgcgcaga agcgggcttg ggtgacggtt taggttttgg gttttaggtt
     2701 ttggcgcggg tattggaatg gctaccgcgt gcacggccaa gaactgggat ccgagcggca
     2761 acaccgctag aaactagaaa cgtggtcgaa tcggaggaga cgggcacatg aagctggggt
     2821 tctttacggc ggcgtttccg gggttggatc tggagcaggt ggcgacctgg gcggcggaaa
     2881 gcaatttcca gacgctggag attgcctgct ggccggccgg gaaggcggag cgacggtacg
     2941 cgggtgtcag ccatatcgac gtttcggatc tcgacaagtc cggggcgaag aagatccgca
     3001 aaatgctgaa cggcctgaag ctcgatatct cctcgctcgg cttttatccg aacccgttgc
     3061 acccggatgc ggaggtgcgc gaggcggcct tcgaccacat caagaaggtg atcgacgcgg
     3121 ccgtgctgct tgaggtgccg attgtcggca cgttcatcgg ggccgaccag agcaagagcg
     3181 cggccgagaa cctggcggcc ttcggcctcc tctggccgcc gatcatcaaa tacgcgggtg
     3241 agcgcgggat caagatcgcg atcgagaact gcccgatgct ctggcatgac acctggccag
     3301 gcgggcagaa cgtcgcctac tcgccgacga tctggacgaa gatgttcgag atgatcccgg
     3361 acgagaactt cggcctcaac tacgacccgt cgcacctgat ctggcagttc atcgatgagg
     3421 tcaagccgat ccgcgaattc agggaccgca tcttccacgt ccacgccaaa gacatgtggc
     3481 tggatcgcga aaaactggcg cgggacggca tcctctcggc cggcgtcggc tgggcgatcc
     3541 cacgcttgcc ggggttgggc gagacgcgct ggggcgagct gatcgccgcg ctctacgcca
     3601 acggctacga ctccgtgatc tctatcgagc acgaagaccg cgcgtttgag aagaccgaag
     3661 agctcgtgaa acggggcttc tatttgaccc gagactatct caacccttgg atcgtctgat
     3721 tcgctgagga agtgccctgc atgtctgacc gttttgccgt cggagttgac gtcggcggaa
     3781 ccaacctgaa gtttgcaatc gtcaacgaag ccgggtcggt ggttgctcgt ttgaccattc
     3841 cgactgaggg gcatgagggg cacgaccacg tcttgcagcg gatgatccat ggggtgaacg
     3901 aactgcggtc ccgcgggccg gcgggttgcg cgatcgagtc gatcggggtc ggcgtgcccg
     3961 ggatgctcga catggtcacc gggacgacga tcgacttgcc gaacctggcc gggaagtggc
     4021 cgaacgtggc agtcaagtcg atcatggaag ccgcacttca cgtgccggtg cacatgatca
     4081 acgacgtcaa agccttcacg atcgccgaga gggagttggg cgcggccaag ggcgcgaaca
     4141 atgtcgtctg ctacgcgatc gggaccggga taggcggtgg ggtgttgatc gacggcaagg
     4201 tccacttcgg gatcggcggg gcggccggcg agctcgggca catcatcgtc aatccgggcg
     4261 gtgtgcgctg ctcgtgcggc aactacggct gcgtcgaggc gatggcgagc ggtccggcga
     4321 tcatcggcga ggcgaatcgg cgggtggtcc agggcttcac gacgcggctg accgagatga
     4381 tcggcgacga cctgaaccgg atgacgccgg ggctggtcga gcaagcggcg gcggaaggcg
     4441 atcctgccgc gcgcgaggtg ttggagcgcg ccggatactt cctcgggctg agcatggccg
     4501 ggatgattgc cgcgctggcg ccggacgtgg tcgtgattgg cggcggagtg gtcaagccgc
     4561 acggcgtgta ctggaccgcc ttcgagtcga ccgcgcgggc cagcaacacg gtca
//
LOCUS       DATAOZ010000515         1993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7311161, whole genome shotgun sequence.
ACCESSION   DATAOZ010000515 DATAOZ010000000
VERSION     DATAOZ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7311161"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VHR64_13070"
     CDS             complement(<1..446)
                     /locus_tag="VHR64_13070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3303809.1"
                     /translation="MKRPFEEIVAHHGSTVLRVCRAMLPQPDAEDAWSETFLAALRTY
                     PDLPDSANVEAWLVTIAHHKAIDALRAQSRRPTPVAEIAEANSAARPEEPPDDDLWSA
                     LRALPAKQRQAVAFHHVAGLSYAEVATIIGGSTDAARRAAHDGIRSL"
     gene            707..1081
                     /locus_tag="VHR64_13075"
     CDS             707..1081
                     /locus_tag="VHR64_13075"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3303810.1"
                     /translation="MSEDRLSQVFAALADPTRRDIVARLAVGDATVSQLAAPYNVSIQ
                     AVSKHLKVLQDAGLVSRSHDAQRRPRHLEADVFDMMTAWIERYRKQAEERYRRLDAVL
                     AAMDDNSEPKINQPTINQGAAS"
     gene            1078..1581
                     /locus_tag="VHR64_13080"
     CDS             1078..1581
                     /locus_tag="VHR64_13080"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX3303811.1"
                     /translation="MSTNAKPEAATRETTIEADPDLPTIRIIRDFDAPREKVFRAWIE
                     PELVAQWLGPKSSEMRIDEWNAHTGGNYRYASVANGEEVAAFYGSFHEVRPSVRLVQT
                     FTWEGMPDGVSLETMTFEDLGDGRTRTIGLSLVDNLQARDAIMASGMDVGVNEGYEKL
                     DALLADS"
     gene            1719..>1993
                     /locus_tag="VHR64_13085"
     CDS             1719..>1993
                     /locus_tag="VHR64_13085"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX3303812.1"
                     /translation="MSITTEELRAAYLRSGNVYAGSEKLGRIGQIYLDDETSEPTWVT
                     VKTGLFGASESFVPLNSARLEGDDILVDYDKDTIKNAPRIDADGDLTP"
BASE COUNT          413 a          613 c          615 g          352 t
ORIGIN      
        1 agactgcgaa ttccgtcgtg cgcagcgcgc cgcgccgcat ccgtcgagcc gccgatgatg
       61 gttgccacct ccgcgtacga cagccctgcg acatggtgga aggcaaccgc ctgccgctgc
      121 tttgccggga gagcgcgtag ggccgaccac aggtcgtcgt cgggcggctc ctcgggacgg
      181 gccgcggagt tggcttcggc gatctcggca accggggtcg gccggcgact ctgggcgcgg
      241 agcgcatcaa tggccttgtg gtgtgcgatt gtgaccagcc atgcctcgac attggcgctg
      301 tccgggagat ctggataggt ccgcaaggca gcaagaaatg tctctgacca ggcatcctcg
      361 gcgtctggtt gcggcaacat ggcgcgacag acgcgcagca ccgtcgaacc gtggtgcgcc
      421 acgatctctt caaacggccg tttcatcctc atcaaccttc tagacgatgg caccgcgcaa
      481 aacgtgaggt ttgtgaggtt ccgggtgtcc ccttttggca cccgtgctgc gaaacgtacc
      541 tagcgggagg ccgctgcagg ggtcgtcttc gcgactgtgg tggaaaacgt acccgccagc
      601 caccccgtac gcacctttat tcaaccaact ggttgacaac gcgtcagccc tccggttaat
      661 gttgagttca accaattggt tgaataacca gaccaaggag gcggccatga gcgaggatcg
      721 actatcccaa gtcttcgcag ccttggccga ccccacgcgg cgcgacatcg tcgctcggct
      781 cgctgtcggg gacgccacgg tgagccaact ggcggcaccg tacaacgtgt cgattcaggc
      841 ggtgtccaaa cacctgaaag tactccagga cgccggtctg gtcagccgca gccacgatgc
      901 ccagcgccga ccccgacacc ttgaggcgga cgtattcgac atgatgaccg cttggatcga
      961 gcgctaccgc aagcaagctg aggagcgata tcggcggctc gatgccgtac tcgccgccat
     1021 ggacgacaac agcgaaccaa agatcaatca accaactatc aaccaaggag cagcatcatg
     1081 agcacgaacg caaagccgga agccgcgacg cgcgagacca cgatcgaggc tgatcccgac
     1141 ctgccgacca tccgcatcat tcgcgacttc gacgccccgc gcgagaaggt ctttcgggcg
     1201 tggatcgaac ccgaactggt cgcccagtgg ctcggcccga agagcagcga gatgaggatc
     1261 gacgagtgga acgctcacac cggaggtaac tatcgctatg cctcggtcgc gaacggcgaa
     1321 gaggtggccg ccttctacgg ctccttccat gaggtgcggc cgagcgtgcg gctggtccag
     1381 accttcacct gggaaggcat gcccgatggc gtcagcctgg agaccatgac gttcgaggac
     1441 ctcggcgatg gccggaccag gacgatcggt ctctcgttgg tcgataacct ccaggcccgc
     1501 gacgcgatca tggcaagcgg tatggatgtc ggagtcaatg agggctacga gaaactggac
     1561 gccttgctcg ccgatagctg atacccagcc tccgacttgt cagcgtcgta gcaggccata
     1621 acccgctacg acgctgacaa gtttgtttga ggctgtctgt tttgtgatcg atgccgtcgg
     1681 ttaaggaacc ttactagaga cagagaaggg gacgcgccat gagcattacc acagaagagc
     1741 ttcgggctgc ctacttgagg tctgggaatg tgtacgccgg ctcagagaag ctcggccgga
     1801 tcggccagat ctacctcgat gatgagactt ctgaaccaac ctgggtcacg gtcaagactg
     1861 gactattcgg agcctcagag tccttcgtgc cgctgaattc ggcgcggctc gagggcgacg
     1921 acattctcgt cgactacgac aaggacacca tcaagaacgc cccgcggatc gatgccgacg
     1981 gtgatctgac acc
//
LOCUS       DATAOZ010000516         8357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9151718, whole genome shotgun sequence.
ACCESSION   DATAOZ010000516 DATAOZ010000000
VERSION     DATAOZ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9151718"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..431)
                     /locus_tag="VHR64_13090"
     CDS             complement(24..431)
                     /locus_tag="VHR64_13090"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3303813.1"
                     /translation="MTEPAAGHPTIESLAREFFAAIEDRDPRRLHATLAEHATFRALP
                     HSQPIRPANDIVAYFGTVVSSYPSAHWEVSDVIASGDRAAIQFVVREFSTRLGRDLLS
                     EQVVVMRAAQGRIVNIVGYYDSNEFRRVFWEES"
     gene            complement(443..1078)
                     /locus_tag="VHR64_13095"
     CDS             complement(443..1078)
                     /locus_tag="VHR64_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303814.1"
                     /translation="MTTTMHKAAHLGPQPGPTAGDVRIADLSNDSLIIRLHFTINHLS
                     RWLSPIHDRERMDRSSLRGQPSVKELLIGMREEERRVFPMLYLMATQNNPDLDKLPPP
                     VISPEQAVYDRNRTVLSIQSEFRRLRQSTCSLLRSLPDSAWLRVGTSRRVHDWQIRGL
                     AETLVAHDEDLLTQMDLALDRNGLRDSVAEHGRAHLYELLKIVPVTLRDRT"
     gene            complement(1109..1600)
                     /locus_tag="VHR64_13100"
     CDS             complement(1109..1600)
                     /locus_tag="VHR64_13100"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3303815.1"
                     /translation="MDNEQQDQVLRSREEILRALEHFPTEFRRSVLEAADREALLRPA
                     FDGGWGVVEIIPHLRDWEAIYLDRATRILDEDRPALPGFDDTLWSIERDYRGQDPYET
                     FAHFAALRGELVTLLQAATPEQWERVGIHGVYGEITLQWMANHICDHDAEHLQQAREA
                     LTV"
     gene            complement(1678..2133)
                     /locus_tag="VHR64_13105"
     CDS             complement(1678..2133)
                     /locus_tag="VHR64_13105"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX3303816.1"
                     /translation="MNTTRTEPTALLFTATQWQQVVEHLSAVLPAEGCGLLACAEDDH
                     GDLSILRIFPGTNTLASSTRYRMKPSEVIDAFRAMREQGLRLAAIFHSHPATPPTLSE
                     IDLREANYPDAALLIFSFAGPSPEAAAWRLERQEDQWVAAKIPIRIRPA"
     gene            2198..3787
                     /locus_tag="VHR64_13110"
     CDS             2198..3787
                     /locus_tag="VHR64_13110"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX3303817.1"
                     /translation="MVRSQLFRLMLVAAFSVSQLASAAARAELPIASSASVDPDYAPL
                     VDSVEPDYLRTTRDLTRYEVVAELTPVDGQSNASIAGKTTITYQNHTGTSLDEIYLRL
                     YPNSAEYAEGEMTVSRIRVDGQQATGALGQDDTMLTIALPTPAADGAEIKLSFRFKTT
                     IPTDPRRSYGMFKYDTGTNTYSLAHWQPLLAGWDRVHGWNTGPISKNGDPVFTEAAVF
                     DVTLTAPSGLIFATSGSALDQTANGESATTHYASGPSRDFVMAASADFVVTETTVGET
                     TVRSFAWPGSEEGSQRVLESGAKSAKLYNDLIGLYPYEELDLVQVNIGNGAGGVEFPG
                     LVYIGASFYDLASGNVDPDFLEFVVVHEVAHQWFYNVVGNNQYQHAFMDESLANYLSV
                     VYFAAEYSADEANKQANLNLRLGYFDLLFQQGDEVVDQPTDDFPSEHAYGVVIYGKGA
                     LAFMELRREIGADAFFAGLQQYYRDFAFEVAEPEDMKAAFEAASGQDLTEFWTHWFEQ
                     PDGADDFDAADLARLLREIGE"
     gene            3780..4955
                     /locus_tag="VHR64_13115"
     CDS             3780..4955
                     /locus_tag="VHR64_13115"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEX3303818.1"
                     /translation="MNSASEECPTHSPVRDALTFAILTRVALFMMVWISLRAVPRLGL
                     YPAQLPDNFLPSHPFLDGWARWDASHYIAVARFGYGDPASPSPDGGIGFFPLYPLLMR
                     AVATVTGQTDSNGGLAVIGIVISIACFLAATALLAKLASETLPIDDARFAVLLFASAP
                     FAFFYTAAYTESLFVLEVVSAIWFARKGQWWRAAIVAALASATRLVGLAVIGGIIYAA
                     WRSGKRLPNLVGLAAVGASGFIAFMLYLWARFDNPTAYFDTQAKWGGWREHVWYYVKL
                     FLTRPREALQGDPRHLIIIGNVALGLIALALVPVIVRRLDPATAGISVLLIVGQFLIT
                     WVSLGRYLMPAIGIYFAAALLTSTTTRAHWLRETILAISLVTMTALALLYAHGFWVV"
     gene            complement(5036..5815)
                     /locus_tag="VHR64_13120"
     CDS             complement(5036..5815)
                     /locus_tag="VHR64_13120"
                     /EC_number="1.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-iditol 2-dehydrogenase"
                     /protein_id="HEX3303819.1"
                     /translation="MRLEGKTAIVTGGAQGIGRATAKRFAEEGARVVIGDLQEDGAQA
                     VAKEIAGAGGQAVGMALDVRDQSGAQAIVDRAVSEFGSVDILMNNAGVIRITPFLEIT
                     EEEWDLIFDVNNKGLLWCAQAVARQMIKQGRGGKIINVASQAGRRGEALVLTYCASKA
                     AVISMTQSMGLALAEHKINVNAIAPGIVDTPLWESNDRRFAELLGMEIGEPKRTFVKS
                     IPLGRIEQPEDVAGMAAFLASSDADYITQQTYNVDGGNWPS"
     gene            complement(5838..7004)
                     /locus_tag="VHR64_13125"
     CDS             complement(5838..7004)
                     /locus_tag="VHR64_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX3303820.1"
                     /translation="MKITDVTTIKLTYQLDRAMADAIHYMPARPTLLIQVHTDGGFTG
                     LGEAAAYGGSLESMEAVLSDLRQTVLGQDPFTVERLWSRMATQAHQRGRRGMLMMGIS
                     GIDIALWDIIGQATKTPLYRLLGGYRDTIDAYASAGFYARDKDAKELAEEVGGYVERG
                     FRTVKIKVGRNPDVMLNPLHDMNVRDYATTPFEEDIERVRAASKAAGKAKLAIDANNA
                     WTAPVALRFMRAISDIQIAWLEEPVPTEDLAGSAEIARVLDTPVAGYETETGLPGFRN
                     LITNRAVDIVQPDVIWTGGITETRKVAALAQAFHLPVIPHVFSSAVSSIANMHLIAAL
                     PNAGLLEFDQNPNPLRSELFEEPIRVDENGQVHLPEGPGLGVCLNQATIDKYRI"
     gene            7195..8316
                     /locus_tag="VHR64_13130"
     CDS             7195..8316
                     /locus_tag="VHR64_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3303821.1"
                     /translation="MAVARRGLEVEHQTTGSSGAGRSDTKEKVVTIRLSRRTLIKSSA
                     IVGSGLVVGRYYVGPAHAAPPPLAKKDSYTIGFAQTGSTNPWRLAETASMKDQFETKL
                     GWKLIATDANEQTDKQLADIDSIIAQKPDIFIFPPRESKALAPAVLKMKQAGIPVILI
                     DRDVDHDVAKPGEDYVTFIGSDFVQQGMQAAKWLTEAMKGQAKIIELEGTTGADPAID
                     RKKGFDDYIAGTFKGTPTAEGPQGGMQILASQDGNFLRDTGRKVMETLLQAHPDVNAV
                     YAHNDEMAIGAIAALEAAGRKPGQDITLVSIDGENAAMDAIVEGKLGASVESSPFFGP
                     ISYETMMKYVNGEDIPDWVVVKDRFFDKSNAKDFQGKQF"
BASE COUNT         1524 a         2673 c         2600 g         1560 t
ORIGIN      
        1 ccagactcag ctgcaggcgt aactcaggat tcttcccaga agacgcgacg gaattcgttg
       61 gagtcgtaat agccgacgat gttgacgatc cggccctgag cggcccgcat cacgaccacc
      121 tgctccgaga gcaggtcacg cccgaggcgg gtcgagaact cccgcaccac gaactggatc
      181 gcggcccgat ccccgctcgc gataacgtcg ctgacctccc agtgcgccga ggggtatgag
      241 gagacaaccg tgccgaagta ggcgacgatg tcgttggccg gccgaatcgg ctggctatgc
      301 ggcaatgccc ggaaggtggc gtgctcggcc agcgtcgcgt gcaatcgacg cgggtcccgg
      361 tcttcaattg ccgcgaagaa ttcccgggcc agcgactcga tagttggatg cccggcggcg
      421 ggttcggtca tgccgatccc cgctaggtgc ggtcacgaag cgtgactgga acgatcttga
      481 gcaactcgta gagatgcgcg cggccgtgct cggcgacgct atcccgcagg ccgttccggt
      541 ccagcgccaa atccatctgc gtcagaagat cctcgtcgtg cgcgaccagc gtctcggcga
      601 gaccgcggat ctgccagtcg tggactcgcc ggctagtgcc aacccggagc caggctgaat
      661 cgggcaggct gcgcaggagc gaacaggtgc tctggcgcaa acgacggaac tcggactgaa
      721 tcgagagaac ggttcgattg cggtcataca ccgcctgctc gggcgagatg accggcggcg
      781 gaagtttgtc gagatccggg ttgttctgcg tcgccatcag gtagagcatc gggaagaccc
      841 ggcgctcctc ttcgcgcatc ccgatcagta gctccttgac gctcggctgg ccgcgcagcg
      901 atgagcggtc catgcgttcg cgatcgtgga tcggcgacag ccagcgcgac aggtgattga
      961 tcgtgaaatg gagccggatg atcagtgaat cgttggagag atcggcaatc cgcacgtcgc
     1021 cggcggtcgg accgggttga ggaccaagat gagctgcctt gtgcatcgtc gtcgtcatcg
     1081 tctctctcgt gtgtgcctcg cgttcaggct agaccgtgag cgcctcccgg gcctgctgca
     1141 gatgttcagc atcatgatcg cagatgtgat tcgccatcca ctgcaacgtt atctcaccat
     1201 acaccccgtg gatgccaacg cgctcccatt gctcgggcgt ggccgcctga agcaaggtga
     1261 ccaattcacc cctcagcgcg gcgaaatgcg cgaaagtctc gtatggatcc tggccacgat
     1321 aatcgcgctc gatcgaccag agcgtatcgt cgaagccggg caatgccggt cgatcttcat
     1381 ccaggatgcg cgtggcgcgg tcgaggtaaa tcgcttccca gtcccgaagg tgagggatga
     1441 tctcgacaac gccccaaccg ccatcaaagg cggggcgcaa aagcgcttca cgatccgcgg
     1501 cttcgaggac tgatcgcctg aactcggtcg ggaaatgctc cagcgcgcgc aggatctcct
     1561 cacgagatcg caggacctga tcctgctgtt cgttatccac tcaaccgccc tgtttccaaa
     1621 cccaacgctc ccgcctaccc tgcgggcacg cgcgcacaag catacacaac ccgcatatca
     1681 ggctggccgg atgcgaatcg gtatctttgc cgcaacccat tggtcctcct ggcgctccaa
     1741 acgccatgcg gcggcttcgg gagatgggcc ggcaaagctg aagatcagga gcgcggcatc
     1801 ggggtagttg gcttcgcgca ggtcgatttc cgagagtgtc ggcggggtcg cgggatggct
     1861 gtggaagatc gcggccaatc gaagaccctg ctcccgcatc gcacggaagg catcgatgac
     1921 ctcggacggc ttcatccggt accgggtcga cgacgcgagc gtgttggtgc cggggaaaat
     1981 gcggaggatg gacaggtcgc catgatcgtc ctcggcgcag gccagcaacc cgcagccctc
     2041 ggccggcagc accgcgctca ggtgttccac cacctgctgc cactgggtgg ccgtaaagag
     2101 caatgctgtc ggctccgtac gcgtagtatt cacctgaccg gggatcaatt cgctcaccgg
     2161 tagcctcggc gaaatgacgg cagcggggtt cctttcgatg gtgcggtcac aactatttcg
     2221 cctcatgctc gtggccgcat tctcggtctc gcagcttgcc tccgccgcgg cacgggccga
     2281 actacccatt gcgtcatcgg catcggtcga cccggactac gcgccgctgg tcgacagcgt
     2341 cgaacccgat tatctccgaa cgacgcggga cctgacccga tacgaggttg tcgccgagtt
     2401 gacgccggtt gacggccagt ccaacgcgag tatcgccggc aagaccacga tcacgtatca
     2461 gaaccacacc ggtacgtcgc tcgacgagat ctatcttcgc ctctacccga actccgccga
     2521 gtacgccgaa ggagagatga cggtctcgcg tattcgcgtc gatggtcaac aagcgaccgg
     2581 cgcactcggg caggatgaca cgatgttgac tatcgccctg ccaacaccgg cagccgatgg
     2641 cgcagaaatc aagctctcgt tccgattcaa gaccacgatt ccgaccgatc ctcgccgcag
     2701 ctacggcatg ttcaaatacg acacgggcac caatacctac tcgctcgccc actggcagcc
     2761 gctgctcgcg ggttgggacc gcgttcacgg ctggaatacg gggccgatca gcaagaacgg
     2821 cgatccggtc ttcaccgagg cggcagtctt cgacgtcacc ctgaccgcac cgtccgggct
     2881 gatcttcgcc acctccggct ccgccctcga ccagaccgcg aacggcgaga gcgcgacgac
     2941 ccactacgcc agcggaccga gccgggactt cgtgatggcg gcatcggccg acttcgtcgt
     3001 gactgagaca actgtcggcg agacgaccgt tcggtcattc gcctggccgg gatcagagga
     3061 gggcagccag cgggtactcg agagcggagc gaagtcggcc aagctctaca acgacttgat
     3121 cggactctac ccctatgagg agctggacct ggtccaggtc aatatcggca acggcgccgg
     3181 cggagtcgag ttcccagggc tggtctatat cggcgcgtcc ttctacgacc tcgcctccgg
     3241 caatgtcgat ccggacttcc tcgagttcgt cgtcgtccac gaagtcgcgc accagtggtt
     3301 ctacaacgtt gtcggcaaca accagtacca gcacgccttc atggacgagt cgctcgccaa
     3361 ctacctgagc gtcgtctact tcgcggcaga gtacagcgcg gacgaggcaa acaaacaggc
     3421 gaacctcaat ttgcgcctcg gctatttcga tctcctgttc cagcagggcg acgaagtcgt
     3481 cgatcagccg accgacgact tcccatccga gcacgcctac ggagtcgtga tctacggcaa
     3541 gggcgcgctg gcctttatgg agctccggcg cgagatcggc gccgacgcat tctttgccgg
     3601 cttgcagcag tactaccggg acttcgcgtt cgaggtcgcc gagccggagg atatgaaggc
     3661 cgccttcgag gcggcatcgg gacaggatct gaccgagttc tggacgcact ggttcgagca
     3721 acccgatggc gcggacgatt tcgacgccgc cgatctggcc agactgctgc gagagatcgg
     3781 tgaatagcgc ttcggaggaa tgtccgacgc actcgccggt gcgggacgcg ctgaccttcg
     3841 cgatcctgac ccgcgtcgcg ctcttcatga tggtctggat cagcctgcgc gcggtgcctc
     3901 gccttggcct gtatcccgcc cagctcccgg acaattttct gcccagtcat ccatttctcg
     3961 atggctgggc gaggtgggac gcctcccact acatcgccgt ggcgcggttc ggctacggcg
     4021 atccggccag ccccagtccg gacggaggga tcggcttctt cccgctctat cccctgctga
     4081 tgcgggcggt cgcgacggtg accggtcaga ccgattccaa cggcggtctg gcggtgatcg
     4141 ggatcgtaat cagcattgcc tgcttcctgg cggcgacggc gctgctggcc aagctggcgt
     4201 ccgagacatt gccgatcgat gatgcccggt ttgcggtcct gctcttcgcg tcggccccat
     4261 tcgccttctt ctataccgcc gcctacacgg aatctctctt cgtcctggag gtcgtttcgg
     4321 cgatatggtt cgcccgtaag gggcagtggt ggcgggcggc gattgtcgcc gcgctggcgt
     4381 cggcgacgcg acttgtcggg ctggcggtca tcggcgggat tatctacgcg gcgtggcgat
     4441 cggggaagcg attgccgaac ctggtcgggt tggcggcggt cggcgcgagc ggatttatcg
     4501 ccttcatgct ctacctctgg gcgaggttcg ataacccgac cgcctacttc gacacgcagg
     4561 cgaagtgggg cggctggcgc gaacacgtct ggtattacgt aaagctcttt ctgacacggc
     4621 cgcgcgaggc gctccagggc gatccccgcc atctgatcat catcggcaac gtcgcgctgg
     4681 ggctgatcgc gctggccctg gtgccggtga tcgtccgccg actcgatccg gccacggccg
     4741 ggatctccgt cctgctgatc gtcggccagt tcctgatcac gtgggtctcg ctcggtcgtt
     4801 acttgatgcc cgccatcggc atctacttcg cagccgcact gctgacctcc acgacgaccc
     4861 gcgcccattg gctgcgcgag accattctcg cgatctcgct ggttaccatg accgccctcg
     4921 ccctcctcta cgcccacggc ttctgggtcg tgtgagcttt accaggatcg aaacctagcc
     4981 cgaggcatgg cggccccgag ctactaggtc aaaagagaat actgctgatc aggttctagc
     5041 tcggccaatt gccgccgtcg acgttgtagg tctgctgcgt gatgtaatcg gcgtcggaag
     5101 acgcgaggaa tgcggccatg ccagcgacat cctccggctg ctcgattcga ccgagcggaa
     5161 tgctcttgac gaaggtccgc ttcggctcgc cgatctccat cccgagcagc tcggcaaagc
     5221 ggcggtcatt cgactcccag agcggcgtgt cgacgattcc cggcgcgatc gcattgacat
     5281 tgattttgtg ctcggcgagg gcgagtccca tcgactgggt catgctgatc accgccgcct
     5341 tgctcgcaca gtaggtcagg accagcgcct caccgcggcg acccgcttgc gaggcgacat
     5401 tgatgatctt gccgccgcgc ccttgcttga tcatttgccg cgccacagcc tgcgcgcacc
     5461 aaagtagccc cttgttgttg acgtcgaaga tgagatccca ctcctcctcg gtgatctcga
     5521 ggaacggggt gatccggatg acgccggcat tgttcatcag gatgtcgaca ctgccgaact
     5581 ccgagacggc ccggtcgaca atcgcctgcg ccccggattg gtcccggaca tcgagcgcca
     5641 tgccaactgc ctgaccaccg gcgccggcaa tttccttcgc gacagcttga gcgccatcct
     5701 cctgcagatc gccgatgacg actctcgcgc cctcctcggc aaagcgcttg gcagtcgccc
     5761 gcccgatccc ctgcgcgcca ccggtcacga tcgctgtctt gccctcgagt cgcatggtct
     5821 cctccgtttc gctggcgtca gatccggtac ttgtcgatgg ttgcctgatt gaggcaaacg
     5881 cccaggcccg gtccttccgg taaatgaacc tggccattct cgtcgaccct gatcggctcc
     5941 tcgaacagct ctgagcgcaa cggattcggg ttctggtcga actcgagcag cccagcattc
     6001 ggtagtgccg cgatcaggtg catgttggcg atcgagctga ccgccgagct gaaaacatgc
     6061 gggatgaccg gcagatgaaa cgcctgcgcc agagcggcga ccttgcgggt ctcagtgatg
     6121 ccacccgtcc agatcacgtc cggctggacg atgtcgaccg cgcggttcgt gatcaggttg
     6181 cgaaagccgg gcaggccggt ctccgtctcg taccctgcga ccggcgtgtc gagtacgcgg
     6241 gcgatctcgg cacttccggc caggtcttca gtggggaccg gctcctccag ccacgcgatc
     6301 tggatgtcgc tgatcgcgcg catgaatctg agcgcgaccg gagcggtcca ggcgttgttg
     6361 gcatcgatgg cgagtttcgc cttgccggct gccttgctgg ctgcccggac acgctcgatg
     6421 tcctcctcga acggcgtcgt cgcgtagtcc cgcacattca tgtcgtggag cgggttgagc
     6481 atcacgtccg ggttgcggcc gaccttgatc ttgaccgtgc ggaacccgcg ttcgacgtaa
     6541 ccaccgacct cttcggccag ctctttggcg tccttgtccc gggcgtagaa gccggccgac
     6601 gcgtaggcat cgatcgtgtc ccggtagcca ccgaggagcc ggtagagcgg ggttttcgtt
     6661 gcctggccga tgatgtccca gagggcaatg tcgatcccgc tgatacccat catcagcatc
     6721 ccgcgacggc cacgctggtg tgcctgggtc gccatccggc tccatagtcg ctcgacggta
     6781 aagggatcct ggccgaggac ggtctgccgg agatcactga gcaccgcctc catgctctcg
     6841 agcgaaccgc cataggccgc agcctcgccg agtccggtga atccgccatc ggtgtggacc
     6901 tgaatcagca gcgtcgggcg ggcgggcatg taatggatcg cgtcggccat cgcgcggtcc
     6961 aactggtagg tcagcttgat ggtggtgacg tcggtgattt tcatcgaacc cccgagggcc
     7021 cggctgggcg atacgctgat tcccagccag gttgcaggcg aggaaatgtc gtgagcgata
     7081 tcgtagcgaa tttatgcttg acccaaggtc acatcggatt acaatcgccc tacgtgctcc
     7141 aaaatcaggc cgtctctttt gctggtgacg taagcggagg tgaaccacac tgcgatggcg
     7201 gtcgcacgcc gtggcctcga agtggagcat cagacgaccg gctcaagcgg ggccggccgg
     7261 agtgacacga aggagaaggt cgtgactatt cgcctgtctc gtcgcactct gatcaagtcg
     7321 tccgccatcg tcggcagcgg actcgttgtc ggccggtatt acgtgggacc ggcgcacgcc
     7381 gctccgccgc cgctcgccaa gaaggatagc tacaccatcg gctttgccca gaccggcagc
     7441 acgaacccat ggcgcctcgc cgagacggca agcatgaagg accagttcga gaccaagctc
     7501 ggttggaagc tgattgcgac cgatgcgaat gagcagaccg acaagcagct tgccgatatc
     7561 gactcgatca tcgcccagaa gccggatatc ttcatcttcc cgccgcgcga gtcgaaggct
     7621 cttgctcctg ctgtcctcaa gatgaagcag gccggcatcc cggtcatcct gatcgaccgc
     7681 gatgtcgacc acgacgtcgc caaaccgggc gaggattacg tgacgttcat cgggtcggac
     7741 ttcgtgcagc agggcatgca ggccgccaag tggcttaccg aagccatgaa ggggcaagcc
     7801 aagattatcg agctcgaagg caccaccggc gccgatccgg caatcgaccg caagaaggga
     7861 ttcgacgact acatcgccgg taccttcaag ggcacgccga ctgccgaagg accgcagggt
     7921 ggcatgcaga tccttgcgtc tcaggacggc aacttcctcc gcgacacagg ccggaaggta
     7981 atggagacgc tgctccaggc gcacccggac gtcaacgctg tctacgcgca caacgacgag
     8041 atggcaatcg gagcgatcgc tgcgctcgag gcggcgggcc gcaagccggg ccaggatata
     8101 acactcgtct cgatcgacgg cgagaatgct gcgatggacg cgattgtcga gggcaagctt
     8161 ggcgcgtcag tcgagtcgag cccgttcttc gggccgatct cgtatgagac gatgatgaaa
     8221 tacgtcaacg gcgaggatat ccctgattgg gtcgtcgtca aggaccgctt cttcgacaag
     8281 agcaacgcca aggacttcca gggcaagcag ttctagggag aaaccggtta cctactgatc
     8341 gataggcacg ggaggga
//
LOCUS       DATAOZ010000517         9704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6784580, whole genome shotgun sequence.
ACCESSION   DATAOZ010000517 DATAOZ010000000
VERSION     DATAOZ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6784580"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VHR64_13135"
     CDS             <1..243
                     /locus_tag="VHR64_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303822.1"
                     /translation="ITAVVLRIIFELKLADIVITMTGGGPGGATDTVTSFIYREYRDR
                     SNVGYGTALAQVYLFLIIIFILTLLSVVTRWMRRFS"
     gene            283..1206
                     /locus_tag="VHR64_13140"
     CDS             283..1206
                     /locus_tag="VHR64_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3303823.1"
                     /translation="MALPIERTYRSTGFDTRKLKRVTGRVLIYAALLFWTFISLFPIY
                     WTITTSFKVAVDITQQHVIPWLQYQPDWKGWRSLGLSPDTIGVPSSTRTEFIKRFENS
                     LILSLGASLFAVGIGSIAAYGLARFEYKFGPWRNKDISFWFLSQLILPPVALAMPLLV
                     LYKELALLDTRVGMILIYTLMVLPIVIWIMRDQFNSIPIELEQAALVDGATTWGAFGR
                     IVLPIALPGLVAAFILSVIICWNEYFFAALLTATNAKTLPVMVASQTGSQGINWWAMA
                     ALSTAAIAPLAIMGIFLERYIVKGLTAGAVK"
     gene            complement(1452..3500)
                     /locus_tag="VHR64_13145"
     CDS             complement(1452..3500)
                     /locus_tag="VHR64_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEX3303824.1"
                     /translation="MRRTLSRLAWLYPVRATRWLTFLVLIALEFGWVGLGAAAVGTPL
                     YPDLITTAPSGLYIEKGPDGKYLLRFANTAVNLGGRLEIAVGSGTRDIYQNIYDQNVG
                     GNRVIHQKVGADLIYHPQHNHFHFEDFAKYELLKKDSSGYYRATTRTGSKTTYCILDT
                     LRVGTDGPTKRQYDGCGATVQGLSAGWGDTYIASLFGQWIDLGTKMLPDGKYAIRSTA
                     DPDDKLMELNDFNNVGIAYFTIQNGRLSSDALPPLCSLQTVNGGMTGQGNQVNATVGS
                     PIMMNCLRFGSSEPVEIFWGSVNTTPKAMTTSSSGGAIAAQIVVPQSTLGVHYVIARG
                     QTSGTQVAAVVNTMPSIFVTPKRGAVGTQLAVTLRGYSAAEQVDIRFYKSSSSSTSVG
                     TVAVAGNGSGTATFAIPVTPFGPHAVEGVGLSSGARSRASVVVLPSIVEVPDAVEAGA
                     PFGIALRGFGAGERVSFTLDEAGPTVGTVVTSHSGSTTTRTAILTFPAETAPGDYTLS
                     AIGEISRAKVKVGITVSAPTASEEPVPTPPEETASPTVVPTSPPEASPTSEVASPVPE
                     TPTPESSPNASPVAVAPPDVEVTDMDGDGSEIVTLDGAGSSDADGDQLTFAWSITERS
                     PTTGEPMVTVLSTDSVAKISLPVGEHTVTLTVTDSLGSWASDEVIVRILAPPAPEASP
                     "
     gene            complement(3673..4194)
                     /locus_tag="VHR64_13150"
     CDS             complement(3673..4194)
                     /locus_tag="VHR64_13150"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEX3303825.1"
                     /translation="MSQTIVERAMGVLQLDDATYESIEHDTNATTQAAIVVVVVGIAT
                     GIGGIRDSVANILLGPIGQIISWAIVSALVYFVGTKMIPSGSTEADLGQVLRLMGYAS
                     VPAAANILGFIPYLGALIGFVAAIWGLVCTIKAIKHALEMSTGRAIATGIIASLLAAI
                     PLALIGLGVFVAS"
     gene            complement(4310..6154)
                     /locus_tag="VHR64_13155"
     CDS             complement(4310..6154)
                     /locus_tag="VHR64_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3303826.1"
                     /translation="MSGTDLSQEAAFLPHLMSRRRLIAAIGGVASLSAIGMRAYGQEA
                     SPTPSGSPVASPEASPVGTPVGSPVAEATPIAEPPEMIGNLRVVRDQRPTYTTKPKRG
                     GTLTMVRVGRSNSDFNPASFAQDFQIPSSYLEPLVWIDGVTMEPQPWLASSWSWNKDR
                     TEVEYKLQDGVTWHDGERFSARDVAFSFTVYRDDVYSAAANLFTNMASVEAVDDLTVR
                     VQLSAPDSNWIRNASNQLILQRKQYAAYWEGQPEGQRTLSGFSWSDTEPVGTGQWIVN
                     DYRDNQLDLKRNRNYWNDKWAIASVLQVGYVDDEKTQLSHWHDGSADIVWPVSPKELS
                     TVSDRAGTVYAAETVTTMFAAFNFNNPARTDPNIFVDARVRQALSLGIDRNRYAREIF
                     NGFFRPDVPGTILQPDLVLEGLKNPEYQPDEARKLLEAAGFAVTRSDGLLRYPDGHAL
                     KFDVVVRTGDSPELEAILNSIAADLKAVGVLLEVRALSPERFESVWLTDHAFDMIAFT
                     YSQYPGFTDFDLYGSDWDVRNNVQGFNPGSYHNDKVDRAIARALVADSDETYVSALRA
                     IQRQVNDEDPFALWLGSPLDAVLVQANISGYQPNKVWQGWETSRLWRD"
     gene            complement(6287..7903)
                     /locus_tag="VHR64_13160"
     CDS             complement(6287..7903)
                     /locus_tag="VHR64_13160"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX3303827.1"
                     /translation="MLSDRPLIVASNRGPVTFTRERDGSFTCRKGSGGVVTAVSSIAR
                     DRKPIWVACPMDEGDRLRAELARKSGEVVISPPGENPDFRVRFVLPTKREYHLYYNVI
                     SNPLLWFLQHYLWDTPRSPDITAETWTAWTDGYEAVNRQFAEEIVSICEDSPIEPIIM
                     LQDYHLYLTPGFIREMRPQAILQHFVHIPWPDPDYWRLLPTKIRRQICEGMLGNDVIG
                     FQTRDHARSFMYTCESYVPGVQIDYSGRGVIWNGRRIEVRAYPISIDVEAVRRQAYGR
                     EALNHERYLPNYLNEFTILRVDRVEPSKNLIRGFHAFGRFLEKHPEYLGRVSFVSIMV
                     PSRMEVPEYTDYLDDVSAIVVRLNAQYANVETGWQPIHLILGENYPRALAAMKRYDVL
                     LVNTIIDGMNLVAKEGALLNDRNGVLILSEGAGAVQQLGDDAIIIAPADVEGTADAIY
                     QALTMSPGERRRRADNLRRTVESDDVAKWFREQLWDVVRYRIRDERDPGAALPDGSIA
                     GANEAGIDPAQGRLHVIVSPISSDQNGEPS"
     gene            8131..8469
                     /locus_tag="VHR64_13165"
     CDS             8131..8469
                     /locus_tag="VHR64_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303828.1"
                     /translation="MESADINIPAAEARRCPWCDSFDTSLVHRGYTGPTDEVNQYFTC
                     NNCHKVTYELIAKTAREMRVGRFRAGDIYRDQANQTRYEISRVLKVGLNEYLVYLRPL
                     RATSHKAHHD"
     gene            8543..>9704
                     /locus_tag="VHR64_13170"
     CDS             8543..>9704
                     /locus_tag="VHR64_13170"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX3303829.1"
                     /translation="MTETALGIDRATLHTEAERRRTFAIISHPDAGKTTLTEKLLLYG
                     GAVQLAGSVTARQSQRRAASDWMALEQQRGISITSTILAFPYRGHQVNLLDTPGHQDF
                     SEDTYRTLTAVDSAVMVLDAARGIEPQTLKLFEVCRMRHTPIFTYINKLDRPAKHPLE
                     LLDEIEQTLGMKTVPMNWPIGDGPEFRGVYDLRTKTVHLFERTEHGAKRAPVKVATLD
                     DPSLDTLLGTERAATLRDDIELIGSAGHEFDLDAVLRGELTPVCFGSAITNFGVQLFL
                     DMFVDYAPAPNAYATSDTIVEPDQPEFTGFVFKIQANMDPRHRDRVAFLRVCSGRYSR
                     DMVVWHSRMKRKVRLAPPLRLFGQEREVIEEAYAGDVIGVINPGMFAIGDTVS"
BASE COUNT         1792 a         3068 c         2978 g         1866 t
ORIGIN      
        1 atcaccgcgg tggtgctgcg aatcatcttt gagctcaagc tcgcggacat tgtcatcacg
       61 atgaccggcg gcgggccggg tggcgcgacg gataccgtga caagcttcat atatcgcgaa
      121 taccgggatc gctcgaacgt cggttacggg acggctctgg cgcaggtgta cctgttcctg
      181 atcatcatct tcattctgac cttgttatcg gtcgtgactc gttggatgcg acggttctcc
      241 tagtctgcat cgaggacggt tcaggaaagc aggaacgaga tcgtggcact gccaatcgaa
      301 cgaacgtatc gcagcaccgg gttcgatacc cgcaagctga agcgggtgac cggacgggtt
      361 ctgatctacg ctgcgctgct attctggacc ttcatctcgc tgtttccgat ctactggacc
      421 atcacaacgt cgttcaaggt cgcggtggac attacccagc agcacgtgat tccgtggctg
      481 caataccagc ctgactggaa gggatggcgt tccctgggcc tctcgcccga tacgatcggc
      541 gtcccgtcgt caacccgaac cgagttcatc aagcggttcg aaaacagcct gatcctctcg
      601 ctcggcgcct ccctctttgc ggtcggcatc ggatcgatcg ccgcatatgg cctggcccga
      661 ttcgaataca agttcggccc gtggcgtaac aaggacatct cgttctggtt cctgtcgcag
      721 ctcatcctgc cgccggtcgc gttggcaatg ccgctcctcg tgctttacaa ggagctggct
      781 ctcctcgaca cccgcgtcgg gatgattttg atctacacat tgatggtgct tccgatcgta
      841 atctggatca tgcgggatca gttcaactcg ataccgatcg agcttgagca ggctgccctc
      901 gtggacggcg caacgacgtg gggcgcattt ggccggatcg ttctgccgat cgcgctgcct
      961 gggctcgtcg ccgcgttcat cctctcggtc atcatctgct ggaatgagta cttcttcgcc
     1021 gcgctcttga cggccacgaa tgccaaaacg cttccggtca tggtcgccag ccagaccggc
     1081 tcgcaaggca tcaactggtg ggcgatggca gccctttcca cggcggcaat tgccccgctc
     1141 gcaatcatgg gcatcttcct ggagcgatac atcgtcaagg gcttgacggc aggcgccgtc
     1201 aagtagcgac gcctgcagcc gcgtgcatat accttaacgg tccccggaac gtagcttggg
     1261 gttaccacgc cccgcttggt gcatccccac gcccggctga tggtgatgga ccgtgaccgg
     1321 ggttggttcc cgcaatgccg tgaccggcat ctggtccggg cagggcgtgt ccctctctcc
     1381 ggctctctcc gcaaggaacg agggcatgcc cacgctacga caacgaccgg acgaccgttc
     1441 ttaactccgt tttacggact cgcctcgggt gctggcggcg ccaggattcg cacgatgacc
     1501 tcgtccgagg cccacgaacc gagcgaatcg gtgaccgtca aagtgaccgt gtgctccccg
     1561 actggcaacg agatcttcgc caccgagtcc gtgctcaaga ctgtgaccat cggctcgcct
     1621 gtcgtgggcg atcgctcggt gatggaccag gcgaaggtga gttgatcccc gtcggcatcg
     1681 ctcgaaccgg caccatccag ggtcactatt tccgacccgt cgccatccat atctgtcacc
     1741 tcgacatccg gcggtgcgac cgcgaccggg ctggcgttcg gactggactc cggagtcggc
     1801 gtctcgggca ccggcgatgc gacctcgctg gtcggcgacg cctcaggtgg agacgttggt
     1861 acaactgtgg gcgaggcggt ctcctccgga ggagtcggta ccggttcctc cgatgctgtt
     1921 ggcgcactga ccgtgatccc gactttgacc ttcgccctgc tgatctcacc gatggccgag
     1981 agcgtgtaat caccgggcgc ggtctcagct ggaaacgtca ggatggcggt gcgagtggtc
     2041 gtgctgccgc tgtggctggt cacgactgtc ccgaccgtag ggcccgcctc gtcgagcgtg
     2101 aaactcaccc gttccccggc gccgaatccc cgcagcgcga taccgaacgg cgcgcccgct
     2161 tcaaccgcgt ccgggacttc gacaatgctc ggtaggacaa ccacagacgc ccgcgatctg
     2221 gcgccgctcg acaggcccac gccttcgacc gcatgcggcc caaacggcgt caccggaatt
     2281 gcgaaggtcg ccgttccgct gccattcccg gccacggcga ccgtcccgac cgaggtcgag
     2341 ctgctcgacg acttgtagaa ccgaatgtcg acctgctcag ccgcactgta tccgcgcagc
     2401 gtcacggcca attgcgtgcc gaccgcgccg cgcttcgggg tgacaaagat gctcggcatc
     2461 gtgttgacga cggccgcaac ctgcgtgccg ctggtctggc cacgcgcgat cacgtagtgg
     2521 acgccgagcg tcgattgcgg gacgacgatt tgcgccgcaa tcgccccacc gctcgagctg
     2581 gtggtcatcg ccttcggagt cgtattgaca ctaccccaga agatttcgac gggctcgctg
     2641 ctgccgaacc gcaggcagtt catcatgatc ggcgagccga ccgtggcgtt gacctggtta
     2701 ccctgaccgg tcatgccgcc gttaaccgtc tgcaggctgc aaagcggcgg tagcgcgtcg
     2761 gaagagagtc gcccgttttg gatcgtgaag taggcgatcc cgacgttatt gaagtcgttc
     2821 agctccatca acttgtcgtc cggatcggcc gtcgaccgaa tcgcgtactt gccgtccggc
     2881 agcatcttcg tgcccagatc gatccactgg ccgaagagcg acgcaatata ggtgtcgccc
     2941 catcctgccg aaaggccctg gaccgtggcc ccgcacccgt cgtactgccg cttggtcggt
     3001 ccgtccgttc cgacccggag cgtgtcgagg atgcagtagg tcgtcttcga gccggttcgg
     3061 gttgtagcgc ggtagtagcc ggacgaatcc ttcttgagca gctcgtactt cgcgaagtcc
     3121 tcgaagtgga agtggttgtg ttgcgggtga tagatgagat cggcgccgac cttctggtgg
     3181 atgacccggt tgccgccgac attctggtcg tagatgttct ggtagatgtc tcgcgtgccg
     3241 ctgccaaccg cgatctcgag gcgtccgccc agattgaccg cggtattggc aaatctgagc
     3301 aggtacttgc cgtccgggcc tttctcgatg tagagaccgg aaggcgccgt cgtgatcagg
     3361 tccgggtaga gcggagtgcc gacagcggcc gctcccaggc cgacccagcc gaactcgagg
     3421 gcgatcagga ccaggaacgt cagccatcgc gtagcgcgca cggggtaaag ccacgccagg
     3481 cgagacaagg tgcgacgcat ctgccggtcc tttccagaca accgcggggt tcaacgcctc
     3541 gtgagtgcag agaggatcga tgttcagtta tcgatatgca ctaatcggca gtatagcaac
     3601 cgattccgga catggaaaca gggcggtcga gaaactcgac tgccctgtta aatccaggaa
     3661 atctatctgg acttagctcg ctacgaagac tccgagccca atcaacgcaa gcgggattgc
     3721 ggccaacaag cttgcaatga tgccagtcgc gatagctcga ccggtgctca tctcgagggc
     3781 atgcttgatc gccttgatag tgcaaacgag cccccaaatc gccgcgacaa atccgatcag
     3841 ggcgccgaga tatgggatga atccaagaat gttcgcggcg gcaggtaccg acgcgtagcc
     3901 catcagccga agaacttggc cgagatccgc ctcagtcgag cccgacggga tcatctttgt
     3961 ccctacgaaa tacacgagtg ccgagacaat ggcccagcta atgatctgcc cgatgggccc
     4021 cagcaggata ttcgcaacac tatcgcgaat tccgccgata cccgtcgcaa ttcccacgac
     4081 gacgacaacg atcgccgcct gggtcgtggc gttagtgtca tgctcgatcg actcataggt
     4141 ggcgtcgtcc aattggagga cacccatagc gcgctcgaca atggtttgcg acaaggcaga
     4201 ttgatttaac acggcgagag gacctccatc tcagcagaac tcgatcatcg ggcgcgggtg
     4261 cgcgctcatg ataagacaag ttttgcctct gtgaaatacc tgtaggaaat taatctcgcc
     4321 aaaggcgact ggtttcccat ccctgccaga ccttgttggg ctggtatccg gaaatattgg
     4381 cctgaacgag caccgcgtcg agcggggaac cgagccagag cgcaaaggga tcctcgtcgt
     4441 tcacctggcg ttggattgcg cgtaatgccg atacgtacgt ctcgtcactg tccgccacaa
     4501 gcgcgcgagc gatcgctcgg tcgaccttgt cgttgtggta gctgcccggg ttgaagccct
     4561 ggacattatt gcggacatcc cagtccgagc cgtacagatc gaagtccgta aatcccgggt
     4621 actgggagta ggtgaacgcg atcatgtcga aggcgtgatc ggtcagccag acgctctcga
     4681 accgctcagg ggagagggca cgcacctcca gcaacacgcc gactgccttg aggtccgcgg
     4741 cgatgctgtt caggatcgct tcgagctccg ggctatcccc ggtgcggact acgacgtcga
     4801 acttcagtgc atgtccgtcg gggtaccgca gcagtccgtc gctgcgggta acggcaaatc
     4861 cggctgcctc gagaagcttg cgcgcttcgt ccggttggta ttcggggttc ttcaacccct
     4921 cgagcacgag atccggctgg agaatcgtcc ccggcacgtc tggccggaaa aatccgttga
     4981 agatttcccg cgcgtaccgg ttgcggtcga taccgagcga cagcgcctgc cggactcggg
     5041 cgtcgacgaa gatattcggg tcggtccggg ctgggttgtt gaagttgaat gccgcgaaca
     5101 tggtcgtgac cgtctcggcg gcgtacacag tcccggcgcg atcactgacc gtgctcagct
     5161 ccttcggcga gaccggccag acgatgtcgg cgctgccgtc gtgccagtgc gagagctggg
     5221 tcttctcgtc atcgacgtaa ccgacctgaa ggacgctcgc gatggcccac ttgtcgttcc
     5281 agtaattgcg gttgcgcttg aggtctaact ggttgtcccg gtagtcgttg acgatccact
     5341 gcccggttcc gaccggctcg gtatcgctcc aactgaagcc gctcaaggtg cgctggccct
     5401 cgggctgacc ttcccagtac gcggcgtact gcttgcgctg cagaatgagc tggttggacg
     5461 cgtttcggat ccagttggag tccggcgccg aaagctgaac cctgacggtc aggtcatcga
     5521 ccgcctcgac tgacgccatg ttcgtgaaga ggttcgcggc ggccgagtag acgtcgtcgc
     5581 ggtagacggt gaacgagaag gcgacatccc gcgcgctgaa tctctcgccg tcgtgccagg
     5641 tgacgccgtc ctggagtttg tactcgacct cggttcggtc cttgttccag gaccaggagg
     5701 aggcgagcca cggctggggc tccatcgtca caccatcaat ccagaccagc ggctccaggt
     5761 agctgctcgg aatctggaag tcctgcgcga aggatgcggg gttgaagtcc gaattcgagc
     5821 gaccgacgcg caccatggtc agcgttccgc cgcgcttggg tttcgtcgtg taggtcggcc
     5881 gctgatccct gacgacgcgc agattgccga tcatctcggg cggctcggcg attggtgtcg
     5941 cctccgccac cggggatccc accggtgtcc cgaccggact ggcctcgggc gatgcgaccg
     6001 ggctgccgga tggcgtaggt gaggcttcct gcccatacgc ccgcatcccg attgccgaga
     6061 gcgaggcgac gccgccaatt gcggcgatca ggcggcgacg gctcatgaga tgcggaagaa
     6121 aggcagcttc ctgggagaga tcggttccgg acaccggctg ggtacgctcc tacgtcgttc
     6181 acaaaaaccg tcccgtcaaa tcgatcgacg ggacggatca gttgcagatt cagctacgcg
     6241 ccagaacggg atcggcacgt gttcctcaaa gtctacgccg tcgcggtcag gatggctctc
     6301 cgttctggtc cgaagagatc ggcgaaacga tcacgtgaag ccgtccctgg gccggatcga
     6361 tgcctgcttc gttggctccc gcgatcgacc cgtccggcag cgccgccccc gggtcgcgct
     6421 catcccggat gcggtagcgg accacgtccc agagctgctc gcggaaccac ttcgcgacgt
     6481 cgtcgctctc gacggtgcga cgcaggttgt cggcccgccg ccgccgctcg ccgggcgaca
     6541 tagtcagcgc ctggtagatc gcatcggccg tgccttcgac atcggcaggc gcgatgatga
     6601 tggcatcgtc gccgagctgc tggacggctc cggcgccctc ggacaggatc aggacgccgt
     6661 tgcggtcgtt cagcaacgcg ccttccttgg ccaccaggtt catgccatcg ataatcgtat
     6721 tgaccagcag cacgtcgtac cgcttcatgg ccgccagagc acgcgggtag ttctcgccca
     6781 ggatcaggtg gatgggctgc catccggttt cgacgttggc atattgcgcg ttgaggcgga
     6841 cgacgatcgc gctgacatca tcgagatagt cggtgtactc gggcacctcc atacgggatg
     6901 gcaccatgat cgatacgaag ctcacccggc cgagatattc cgggtgcttc tcgaggaaac
     6961 gaccgaaagc gtggaagccg cggatcaggt tcttgctcgg ctcgacccgg tcgacgcgca
     7021 ggatggtgaa ctcgttcagg tagttcggta gataccgctc gtgattgagc gcttcccgac
     7081 cgtaggcctg gcgccggacc gcctcgacgt cgatcgagat cgggtaggca cggacttcga
     7141 tccgccgtcc gttccagatc accccgcggc cgctgtaatc gatctgcacg cccggcacgt
     7201 acgactcaca ggtgtacata aagctgcgag catggtcgcg cgtctggaac ccgatcacgt
     7261 cgttgccgag cataccctcg cagatctgcc gccgaatctt cgtcggcagc agacgccagt
     7321 agtcaggatc cggccacggg atgtgaacga agtgttgcag gatcgcctgt gggcgcatct
     7381 cccggatgaa gcccggcgtg aggtagaggt gatagtcctg gagcatgatg atcggctcga
     7441 tcggcgagtc ctcacagata ctgacgatct cctcggcgaa ctggcggttg acggcctcat
     7501 agccatcggt ccaagccgtc caggtctcgg ccgtgatatc gggcgagcgc ggcgtgtccc
     7561 acaggtaatg ctgcaagaac cagagcaggg gattgctgat cacgttgtag tacaggtggt
     7621 actcgcgttt ggtcggcaac acaaagcgga cccggaagtc cgggttttcg cccggcgggc
     7681 tgatgacgac ctcgccgctc ttgcgggcca actcagcccg gagccgatcc ccttcgtcca
     7741 tcggacaggc gacccagatc ggcttgcgat cgcgcgcaat cgagctgacc gccgtgacca
     7801 cgccgcccga gcccttgcga cacgtgaacg acccatcgcg ttcgcgggta aatgtcaccg
     7861 gtccacgatt cgaggctacg atcagcggac gatcactgag cacgtcccca ccgttactgt
     7921 ctaaatttgt atcgtcctga gtggacatgc tcgcccctcc tgatctgcgt gcgccgcgtg
     7981 cgcggagcga tagcccggat agctcaatta tgcctgctgg aggcaaccat cgcagagtcg
     8041 cggccacaca ggctggtata cttccgccag atcgtcccgg catgatgcac tgagactgat
     8101 gcagaggtcc cgcgcgggtc aatgaacgct ttggaatctg ctgatatcaa tatcccggca
     8161 gccgaggctc gtcggtgccc gtggtgtgac agcttcgata cgtcgctcgt ccatcgcggc
     8221 tacaccgggc cgacggatga agtcaaccag tacttcacct gcaacaactg tcacaaggtc
     8281 acctacgaac tgatcgccaa gaccgcccgc gagatgcgcg tcggtcgctt ccgcgccggt
     8341 gacatttacc gggatcaagc gaaccaaacc cggtatgaga tcagccgcgt cctcaaggtc
     8401 ggtctcaacg agtacctcgt gtacctccgc ccgctgcggg caacatcgca caaagcccat
     8461 cacgattagc taagcctgcg ctctccctcc ttggcagcga tgttcgctgt cctcgttttt
     8521 gtttgcctga aagagattac ccatgactga aacagcgctc ggcatcgacc gggcaaccct
     8581 ccatacagag gcagaacgcc gccggacctt cgcgatcatc tcgcacccgg atgccggcaa
     8641 gacgacgctg accgagaagc tgctgctcta cggaggcgcg gtccagctcg ctggctccgt
     8701 caccgcacgc caaagccaac gccgcgcggc ctccgactgg atggcgcttg agcagcagcg
     8761 cgggatctcg atcacgtcga cgatcctcgc cttcccgtat cgcggccacc aggtaaacct
     8821 gctcgacacc cccggccacc aggacttttc ggaagacacc taccgaacgt tgaccgccgt
     8881 cgacagcgcg gtaatggtgc tggatgcggc caggggcatc gagccgcaga ccctgaagct
     8941 cttcgaggtc tgccggatgc ggcacacgcc gatcttcacc tacatcaaca agctcgatcg
     9001 cccggccaaa cacccgctcg agctgctcga tgaaatcgag cagaccctcg gcatgaagac
     9061 cgtgccgatg aattggccga tcggcgacgg gccggagttc cgcggcgtct acgatctgcg
     9121 gacgaagacg gtacacctct tcgagcggac agaacacggg gcgaagcggg cgccggtcaa
     9181 ggtcgcgacg ctggacgacc cctcgctcga tacgttgctc ggtaccgaac gcgccgccac
     9241 cctccgggac gacattgagt tgatcggcag cgcgggtcac gagttcgacc tggacgcggt
     9301 gctgcggggc gagttgacgc cggtctgctt tggcagcgcg atcaccaatt tcggcgtgca
     9361 gctcttcctc gatatgttcg tcgactacgc gccggcgccg aacgcgtacg cgacctccga
     9421 cacaatcgtg gaaccggacc agccggagtt caccggcttc gttttcaaga tccaggcgaa
     9481 catggatccg cgccaccgcg accgtgtcgc cttcctgcgc gtctgcagcg gccggtactc
     9541 gcgcgatatg gtggtctggc actcacggat gaagcgcaag gttcgcctcg cgccaccgct
     9601 gcgcctcttc ggccaggagc gcgaggtgat cgaggaggcg tatgcgggcg acgtcatcgg
     9661 cgtgatcaac cccggcatgt tcgcgatcgg cgataccgtc agcg
//
LOCUS       DATAOZ010000518         6398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_732044, whole genome shotgun sequence.
ACCESSION   DATAOZ010000518 DATAOZ010000000
VERSION     DATAOZ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732044"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VHR64_13175"
     CDS             complement(<1..1234)
                     /locus_tag="VHR64_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX3303830.1"
                     /translation="MIENVQTGADSFPWWKRGVIYQIYPRSFQDSNGDGTGDLRGITE
                     RLGYLSWLGIDAFWISPFYPSPMADFGYDVSNYCDVDPLFGTLADFDALIEEAHARGL
                     KVIIDFVPNHTSDQHPWFIESRSSRDNPKRDWYIWRDPKPDGSPPNNWLATFGGPAWT
                     LDEATGQYYHHGFLPQQPDLNWRNPEVKEAMLEAIRFWLRRGVDGFRFDVAHHIMKDP
                     ELRDNPKNETPGRLHKPMGEFDTQLHVMDRMHADLHPTYQEIRAVIDSFNTETQPRFM
                     IGETHVFEPVKWATMYGQNLDEMHMPANFGLLKAQWNAKSVREHVDSIEAALPAGAWP
                     NYVLSNHDDWRTATRVGPAQAKVAMMLLLTLRGTPTIYYGEELGMENVEIPPHMEQDP
                     AGLRVPGIGLGRDPERTPM"
     gene            1484..1969
                     /locus_tag="VHR64_13180"
     CDS             1484..1969
                     /locus_tag="VHR64_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX3303831.1"
                     /translation="MATRAEVLADRIASGAEEFIAAIEGLSDEQWQTPCGDERRSVGV
                     LVHHVGTMYPIEADVVRMLAENGEAPGVDWPAVHGINADHATANAAADKAAAIALVRT
                     NVRAAVDAVRGLSDAQLDRVAPNGLAWNAPMTVQNFIENHPIAHPYSHLDSIRAALNG
                     A"
     gene            complement(2252..3217)
                     /locus_tag="VHR64_13185"
     CDS             complement(2252..3217)
                     /locus_tag="VHR64_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303832.1"
                     /translation="MATATTALIPPAEIAAKTAKVEGFWGSALRQLTRNKLAVVSAVF
                     IVVLGLSAIIVPEIYPRTYAEQNYDAITQGPSWQYPMGTDQLGRDIFIRLIFGARISL
                     TVGLVVQAVILLIGVPAGLAAGFFGGKVDTVIMRIVDILYAMPSLLFVIIIMTYLRGT
                     LNSNPGGFWEHLSKIDQATGGLVGVYIGLGLIGWLTVSRIVRANTLSLKSQDFSTAAQ
                     SLGASRSRIMFRHLLPNTVAPIVVATTLGIPSAILSESGISFLGLGVIPPMPSWGLMI
                     SESIVSMRSHPYMLIFPALALSLTVLAFNFLGDGLRDALDPWMKR"
     gene            complement(3220..4152)
                     /locus_tag="VHR64_13190"
     CDS             complement(3220..4152)
                     /locus_tag="VHR64_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303833.1"
                     /translation="MGNRGAYIVRRVLMLIPTLLVIYTLTFVLIHATPGGPWSQGDKP
                     VPPLVLKRLNAAYGLDKPLWRQYTDYLWNVLHGDFGPSFTSRSRTVTDIIKDTFPISL
                     QLGIVAMMLAVVAGITLGVIAAINHNRTADYVCGFISIAGISTPSYVTVSVLVLLLSS
                     QLHLVPTGGWNGITDTRVIIPAFALALYPAAVLARYTRSSMLDVLSADYVRTARAKGL
                     NETVVITRHAIRNALLPVVTVGAIILADVVTGSFYVETIYQVPGIGRYFVDSITSRDY
                     PVILGTVLLFGALVSIMNLIVDILYPMLDPRMSN"
     gene            complement(4244..5989)
                     /locus_tag="VHR64_13195"
     CDS             complement(4244..5989)
                     /locus_tag="VHR64_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3303834.1"
                     /translation="MTDLKDRSPFRKPLTQRQLDRRRFTQGLLASGAFTTIAGLGHPM
                     LAGAQATPEAATPEAATPVNQATPIPAFAGELAADQSMRLPNAEPTTMDPGVSYGDDG
                     ELNVFFNIFDGLVGIDQQTGAVVPRVAESWDINPDATQFIFHIRQGVTWSDGTPLNAN
                     DFVYSWSRVLDPNTLSQYIPAMYPIKNAEKIANGEADISEFGVKAVDDYTLQVDLEAP
                     TTFFPLLASTWTFYPVPKHVIDAKGDQWVEAENIVSNGPFKMTEWSHDQQIVLERNDS
                     YYGEKPTLTKATYTIFSDPTAQAYVAYENNELDYCAPGGPDLERIYADPAKNAELVRF
                     QLSNCYFVVADCSHEPTSKVEFRQALSKAISRETLTKTILKDEYEAAYTVLPDNIPGH
                     NPDSAMPEGVDEAKALLQQAGIDPGSITLDFVFLNLAFNKTVAEYLQSAWQENLGIKI
                     TMSPIEDSTYIDWRASRETQPFGVYTGSWGSDFADASNWFNQNFTSTADHYRNHWKND
                     EFDQLCAKAIGNTNTEERDAEYGQAEVILVDQAAIIPLVRGKAVRAVKPYVKDLYFQP
                     VLSYVHLRTIKIAAH"
BASE COUNT         1179 a         1847 c         2196 g         1176 t
ORIGIN      
        1 gcatgggtgt gcgctccggg tcgcggccga ggccgatccc ggggacgcgc aggccggccg
       61 ggtcctgctc catgtgcggc ggaatctcga cattttccat gccgagctcc tcgccgtaat
      121 agatcgtcgg cgtgccgcgc agggtcagga gcagcatcat cgcgaccttg gcctgggccg
      181 gaccgactcg ggtcgcggtg cgccaatcgt cgtggttgct gagcacgtaa ttcggccagg
      241 cgccggccgg cagggcggcc tcgatcgaat ccacatgctc gcgaacgctc tttgcgttcc
      301 actgcgcctt gagcagtcca aagttggccg gcatgtgcat ttcgtcgaga ttctgaccat
      361 acatcgtggc ccacttgacc ggctcgaaga cgtgcgtctc gccgatcatg aagcggggct
      421 gcgtctcggt attgaacgag tcgatgacgg cgcggatctc ttgatacgtc gggtggagat
      481 cggcgtgcat ccggtccatc acgtggagct gagtatcgaa ctcgcccatc ggcttgtgga
      541 gcctgcccgg ggtctcattc ttcgggttat cgcgcagctc gggatctttc atgatgtggt
      601 gggcgacatc gaaccggaag ccatcgacac cgcgccgcag ccagaagcgg atggcctcga
      661 gcatcgcttc cttgacctcg gggttgcgcc agttcaggtc gggctgctgg ggcagaaaac
      721 cgtggtggta gtactggccc gtcgcctcgt cgagggtcca ggccggaccg ccgaatgttg
      781 ccagccagtt gttgggcgga gagccatccg gcttcggatc tcgccagatg taccagtcgc
      841 gcttcggatt gtcgcggctg gagcgggact cgatgaacca gggatgctgg tcggaggtgt
      901 ggttcgggac gaagtcgatg atgaccttga ggccgcgggc gtgtgcttcc tcgatgagcg
      961 cgtcgaaatc ggcaagggtg ccaaagagcg gatcgacatc gcagtagttg gagacgtcat
     1021 agccgaagtc ggccatcggc gacggataga acggcgagat ccagaaggcg tcgataccga
     1081 gccagctcag gtagccgagc cgctccgtaa tgccgcgcag gtcgccggtg ccgtcaccgt
     1141 tgctgtcctg gaagctgcgc gggtatatct gatagatgac gccgcgcttc caccagggaa
     1201 acgagtcagc gccggtctga acgttttcga tcaattggga atccttgccg aggcactgcg
     1261 tccgcccgta actgtgtaat tctaccgcgt ttgcgcaggt tgattcttga catattgagc
     1321 atccgtcaaa cgtgcgcggc gagaggcaca ctcccccgcc cggcacgaga acgcgcacca
     1381 gcgatgatta aagcccggat gatggactct gcagaatatc ctgctacgat tggcgcactt
     1441 cataatccgt gtcggtgatc ttgactcatg caggaggctt gcagtggcaa cgcgggcgga
     1501 ggtcttggct gatcggatcg cgtcaggcgc tgaggagttc attgcggcca tcgaggggct
     1561 aagcgacgag cagtggcaga cgccgtgcgg cgacgagcgg cgctcggtgg gcgttctggt
     1621 ccatcacgtc ggcacgatgt atccgattga agcggatgtc gtgcggatgc tcgccgaaaa
     1681 cggcgaggcg cccggagttg actggccggc cgtgcacgga atcaatgcgg accacgccac
     1741 cgccaatgcg gccgcagaca aggcagcggc tattgccctg gtgcgcacga atgtgcgggc
     1801 ggcagtagac gccgtgcggg gcctgagcga tgcgcagttg gaccgcgtgg cgccgaatgg
     1861 gctcgcctgg aacgcgccga tgacggtcca gaatttcatc gagaaccacc cgatcgccca
     1921 tccctacagt cacctggaca gcattcgggc cgcactgaac ggcgcataag cgcagcactc
     1981 gaggacaggt acccatccac cagtccgcac cgaggaccag acaacggtac tgggtcgatt
     2041 cacctagatg cgtcagaaat atgggtctga cggtgattgc actggacggg aaaagcggtc
     2101 accgtataag tcgtgaactt tcgccgtccc acgagagaca cgaactcccg gcgccggagc
     2161 aaaggcgccc ggaaccgacc ggcggtggtg tgtagggggc agccacacgc tgccgccggc
     2221 gcgaccgtcc ctgaaccgct cagatgagcg gctagcgctt catccagggg tcgagcgcgt
     2281 cccgcaggcc atcgcccagg aaattgaagg ccaggaccgt tagcgagagg gccagggccg
     2341 ggaagatcag catgtatggg tgcgagcgca tgctcacaat cgactcgctg atcatcaatc
     2401 cccagctcgg catcggtggg atcacgccca gacccaggaa gctgatgccc gactccgaga
     2461 ggatggccga cgggataccg agggtcgtcg cgaccacgat cggagccacg gtgttcggca
     2521 gcaggtggcg gaacatgatc cgggagcgac tggcccccag ggattgggcg gcggtcgaga
     2581 aatcctgcga cttaagcgag agcgtattgg cgcggacgat gcgcgagacg gtcagccagc
     2641 cgatcagccc aagcccgatg tagacgccga ccagaccgcc ggtggcctgg tcgattttcg
     2701 acaggtgctc ccaaaagccg ccggggttgc tgttcagggt gccgcgcagg taagtcatga
     2761 tgatgatcac gaagagcagg ctcggcatcg cgtagaggat gtcgacgatg cgcatgatca
     2821 ccgtgtcgac ctttccgccg aagaagcccg cagccaaccc ggccggtacg ccgatcagca
     2881 ggatcaccgc ctgcacgacc aagccgacgg tcagcgagat tcgcgcgccg aagatgaggc
     2941 ggatgaagat gtcgcggccg agctgatcgg tccccatcgg gtattgccag gatggtccct
     3001 gggtgatggc gtcgtagttc tgctcggcgt aagtgcgtgg gtaaatctcc gggacaatga
     3061 tcgcggagag gccgaggacg acgatgaaga cggccgagac gaccgccagc ttgttcctgg
     3121 tgagctgtcg aagcgcgctg ccccagaagc cctcgacttt ggcggttttg gcagcgatct
     3181 cagcgggcgg gatcagggcg gtcgttgcgg tggccatggc tagttgctca ttcgcggatc
     3241 gagcatggga tagaggatgt cgacgatcag attcatgatc gagaccagcg ccccgaatag
     3301 gagaacggtt ccgaggataa ccggatagtc gcgcgaagtg atcgagtcga cgaagtagcg
     3361 gccgatgccg ggcacctggt agatcgtctc gacgtagaag gagccggtca cgacatcggc
     3421 caggatgatc gcgccgaccg tgacaacggg caggagcgcg ttccggatcg cgtggcgggt
     3481 gatcacgacc gtttcattga gcccctttgc ccgtgcagtg cggacgtaat cggccgagag
     3541 cacgtcgagc atgctcgatc gggtgtacct ggccaggacg gccgccggat agagcgccag
     3601 tgcgaaggcg gggatgatga cgcgggtatc ggtgattccg ttccacccgc cggtcggcac
     3661 gagatggagt tggctcgaga ggaggagcac cagcaccgag accgtcacgt acgacggcgt
     3721 cgagatcccg gcgatcgaga tgaatccgca gacgtagtcg gccgtgcgat tgtggttgat
     3781 cgcggcgatc acgccgaggg tgatgccggc gacgacggcc agcatcatcg cgacgatacc
     3841 gagctgaaga gagatcggga aggtgtcctt gatgatgtcg gtcacggtcc gggatcggga
     3901 ggtgaacgac ggaccgaaat cgccgtggag cacgttccag agatagtcgg tgtactggcg
     3961 ccagagcggc ttgtcgagcc cgtatgcggc attgagccgc ttcagcacga ggggaggcac
     4021 gggcttgtcg ccttgcgacc acggcccgcc cggcgtcgca tggatcagca cgaacgtcag
     4081 cgtgtagatc accagcagcg tcgggatgag catcaagacg cgtctgacga tgtaggcccc
     4141 acgattcccc aagactgctc cgcctgtctt acccggtcac caaccgggct gaaagtcaac
     4201 gcgggcagac cgggatagtc cggtccgccc gcgagtcgaa ctcctagtgc gcggcgatct
     4261 tgatcgtgcg caaatgaaca tacgagagca ccggctggaa gtagagatcc ttgacgtacg
     4321 gcttgaccgc ccggaccgct ttgccgcgca ccagcggaat gatcgctgcc tggtcgacga
     4381 ggatcacttc ggcctgcccg tactccgcat cgcgctcttc ggtattggtg ttgccgattg
     4441 ccttggcgca gagctggtcg aactcgtcgt tcttccagtg gttgcggtag tggtccgccg
     4501 tgctggtgaa gttctggttg aaccagttcg aggcgtcggc gaagtcggag ccccaggagc
     4561 cggtgtaaac gccgaacggc tgggtctccc gggaagcgcg ccaatcgata taggtgctgt
     4621 cctcgatcgg cgacatcgtg atcttgatgc cgaggttctc ttgccaggca ctctgcagat
     4681 actcggcgac cgtcttgttg aacgcgaggt tcaggaagac gaagtcgagc gtgatcgagc
     4741 ccgggtcgat cccggcttgc tggagcagtg ccttggcctc gtcgaccccc tcgggcatcg
     4801 ccgaatccgg gttgtggccc gggatgttgt cgggcagcac cgtgtacgcg gcctcgtact
     4861 cgtccttgag gatcgtcttg gtgagtgtct cgcgactgat cgccttggag agcgcctgcc
     4921 ggaattcgac cttgctggtc ggctcatggc tgcagtcggc gacgacgaag tagcagttcg
     4981 agagctggaa gcgaacgagc tcggcgttct tggccgggtc ggcgtaaatg cgctccaggt
     5041 ccgggccgcc cggcgcgcag taatcgagct cgttgttctc gtaggcgaca taggcctgcg
     5101 cagtcgggtc ggagaagatc gtgtaggttg ccttggtcag cgtcggcttc tcgccgtagt
     5161 agctgtcgtt ccgttcgagc acgatctgct ggtcgtgcga ccactcagtc atcttgaagg
     5221 ggccgttgct gacgatgttc tcggcctcga cccactggtc gccctttgcg tcgatcacat
     5281 gctttggcac cggataaaag gtccaggtgg aggcgaggag cgggaagaag gtggtcggcg
     5341 cttcgagatc gacctggagc gtgtagtcat cgactgcctt gacaccgaac tcgctgatgt
     5401 cggcctcacc gttggcgatc ttctcggcgt ttttgatcgg atacatcgcc gggatgtact
     5461 gcgagagcgt gtttgggtcg agcacgcgcg accagctgta gacaaagtcg ttggcgttga
     5521 gtggtgtgcc gtccgaccag gtcacaccct gacggatgtg gaagatgaac tgggtcgcgt
     5581 cggggttgat gtcccagctc tcggcgacgc gcgggacgac tgcgccggtc tgctgatcga
     5641 tgccgacgag gccatcgaag atattgaaga agacgttcag ctctccgtcg tcgccgtagc
     5701 taacgccggg gtccatagtg gttggctcgg cgttcggcag gcgcatggat tgatcggcag
     5761 ccagctcgcc ggcaaacgcc ggaatcgggg ttgcctggtt gaccggtgtc gcggcctcgg
     5821 gtgtcgcagc ctccggcgtt gcctgggcgc cggccagcat cgggtggccg agcccggcga
     5881 tcgtcgtgaa ggcgcccgaa gccaggaggc cctgggtaaa gcggcggcgg tcgagctgcc
     5941 gttgggtaag cggcttgcgg aacggcgatc ggtctttaag atcagtcatg cacgattctc
     6001 cccaggcggt gcccgccatc acgattcgag gcaaaccagc acggtttgcg ctccgcgatc
     6061 tgcgatgaca aggtccggcg aacgcaatca gaccgcggaa atgcctccgc ggtctgagtc
     6121 gcagtcggat gcatctgcgc gatgtgcgct gccgctagcc gaactgtatg gttcgcccct
     6181 gtcgtcgtga atcaccccgg tagcgtagtc tcgcgccgga gagcatgtca acgacggatc
     6241 atgcaggaac cgcagccccg gcgacgaggc ggccgcggct gtggtcgtac tgggggagga
     6301 ggccttcgac cttgagcagc tcggcgttca ggatcgaggc accggccgcg ccacggatcg
     6361 tgttgtggcc gagcagggtg tacttgatgc cgaggatc
//
LOCUS       DATAOZ010000519         5816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8366408, whole genome shotgun sequence.
ACCESSION   DATAOZ010000519 DATAOZ010000000
VERSION     DATAOZ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8366408"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VHR64_13200"
     CDS             <1..897
                     /locus_tag="VHR64_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX3303835.1"
                     /translation="AVQQRLGTTADDWSTLDELARRAALLRPMTLACATDGNHGRAVA
                     RMAKLLGLGAIILVPVDMAPARIEAIRSEGAEVRMIDGSYDDAVDASAHLASERCMVI
                     SDTAWPGYEDVPRWVIEGYSTILWEVDDALVEIGERGPDLVAIQIGVGALASAVVRHY
                     RRPEVESRPVIVGVEPTRAACMTASIEAGEPVQIPGPHDSIMSGLNCGVPSPLAWPIV
                     SRGIDTFVAIEDERARQAMRDLAASGVVAGECGAAGLAGLQTLLEMGQLPSGAKRALV
                     ISSEGATDRAAYERIVGRISSC"
     gene            complement(1238..2221)
                     /locus_tag="VHR64_13205"
     CDS             complement(1238..2221)
                     /locus_tag="VHR64_13205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303836.1"
                     /translation="MASEPLQPDRALELRRSRWRDFRLDPVLLTFVVLAVVWAVALID
                     IEGYRTLNANLRFIRFASILAIVGIGQTFVVLMGGIDLSVAAVIGLAGVVGGNLISQH
                     GQVAGILMTLAIVFGVGMLNGLGVVLLRLPPLVMTLAASAIIDGALRVYNSGNPVSGN
                     SPFLEKLALGSTWKIPNTAIAALVIAGLGMILLHLTSYGRGVYAIGVNARASLLAGVP
                     VDLTQVVTYGLCSLSAGIVGLILLGQTGYSFQDMGTPYLLQSIAVVVIGGTSILGGRG
                     KLIGTLGGALVMVVLVSWLTALRTTDAERQMIQGILILILLIAYAFTDETR"
     gene            complement(2224..4872)
                     /locus_tag="VHR64_13210"
     CDS             complement(2224..4872)
                     /locus_tag="VHR64_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3303837.1"
                     /translation="MSSSRTATEAASPPDTVPAEQRALTGRAETMTVLETHGLTKAFS
                     GNVVLRNVDFRVRAGEIHALMGENGAGKSTLLKLITGVHAPDEGTIVVNGHSFNRLDP
                     HAAKQQGIATVYQELSLSPHLTVAENIFLGRTPTRSGQIDYRRINRESDEELRALGVE
                     INPEARTDDLSLAERQLVEFVKARMTRPKLLILDEATSALESHQVGLIFGAMRRMAAE
                     GTGIVFVSHRLDEVFAISDRMTVLKNGEYVATVETADTSTAQLVQYMVGRPMEMIFPA
                     KPAIDEVLAQPAVLEVRNLRSGRRFRDVSFELHRGEILGLGGLQGQGQRALLAALFGL
                     HRTDGEVLLNGQAIGSRSPRSAMSRKIAYVPEDRKTEGLLLPQSVKENLTLPNLPQIT
                     HGGLLDRRRENRLVADLVRKLQVRLRSPKQPVLRLSGGNQQKVAIAKWLPLAPDVFLL
                     AEPTRGIDVGTKQEIYTLMRQLADAGASIILISSDTIELLGLSDRVLVMYEHRPVAML
                     AGDDVTEENIVHATVAGRRAGEEEPVPITAESPTARTAPTIEHAPAEAVQRKLSLMER
                     YNRLPQTWQDVGPIFAVLAVFAAIYVYQSRDTIGWHTINNLSTYLLPLMLASMAQSIV
                     MLTGGIDLSLGAMLTLGTALLATRMEDTFASKVGSVLLVLLAGAAIGLFTGSVVSYIR
                     LPAIIVTLATSFIWMGMALYTLPQAGGHLPRSYSTFFKHQIFGIIPVTLVILALALLA
                     WRYIKRSPLGLGIYAVGDNPTGAFYSGVNVRLARIAAYVTAGVFVMLAASALAGYTGT
                     SDPTIGPPYTLMSITAAVLGGVSFLGGQGLLRGAVAGALTFGLLIQILTISGFDPAYQ
                     RIVQGLVLIIALGIKAITTYRLQENR"
     gene            complement(4978..>5816)
                     /locus_tag="VHR64_13215"
     CDS             complement(4978..>5816)
                     /locus_tag="VHR64_13215"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX3303838.1"
                     /translation="QINQIQTMIDQKYDICLLIAGSATALDQVVESATAAGVIVVNWD
                     SLVTTNQLTSKVYEDQIEWGRMTAQWLVEKLGGKGEILAINGPSGIAVSEQRWQGAKE
                     VFDKNPDVKIVGTANIEYNEAPALVQLNSLVTANPSITGIWCQAGAHASAAMKALTQA
                     GMKLVPMTGENFNAFLKQWCKAKDQGFSAFATAEVNYMAVISLDVAVKAKSGEQVPKE
                     VVVPLPRIDDNNVCDWASDEHPDDWYVIPHVPSPEEIDKLIADALSAEMGTPVASPSP
                     AM"
BASE COUNT         1010 a         1775 c         2033 g          998 t
ORIGIN      
        1 gcggtccagc agcggctcgg gaccaccgcc gatgactggt cgacgctcga tgagctggcg
       61 aggcgagcgg cgttgctgcg accgatgacg ctggcgtgcg cgaccgacgg caaccatggc
      121 cgggcggtgg cgcggatggc gaagctgctc ggcctcggcg cgatcatcct ggttccggtc
      181 gatatggcgc cggcgcggat cgaggcgatc cggtcggagg gcgccgaggt gcggatgatc
      241 gacggctcgt acgacgatgc ggtcgatgcc tcggcgcacc tggcatccga gcggtgcatg
      301 gtcatctccg ataccgcctg gccgggctac gaggatgtgc cgcgctgggt gatcgagggt
      361 tactcaacca tcctctggga ggtcgatgac gcactggtgg agatcggcga gcggggaccg
      421 gacctggtcg cgatccagat cggggtcggc gcgttggcct cggcggtcgt gcggcactac
      481 cggcggccgg aagtcgagtc gcgcccggtg atcgtgggag ttgagccgac tcgagcggcc
      541 tgcatgacgg cgtcgatcga ggcgggggag ccggtccaga tcccggggcc gcacgactcg
      601 atcatgagcg ggctgaattg cggggtgccc tctccgctgg catggccaat cgtgtcgcgc
      661 ggcatcgata cgttcgtggc aatcgaggat gagcgggcgc ggcaggcaat gcgcgatctg
      721 gcggcatcgg gagtcgtggc cggggagtgc ggcgcggcgg gtttggccgg tctgcagacg
      781 ctcctcgaaa tgggccaatt gccgtccggt gcgaagcggg cgctcgtcat ctccagcgag
      841 ggcgcgactg atcgggcggc ctacgagcga attgtcggcc gaatttcgag ctgctgaaca
      901 tcgcaatggc ggggctccgt acccgcctgg acttctcgga aaccccttgg cctacgtcaa
      961 cacctggatg tagcgcgggg ttaccatgcc ccgcccggac cccaggacgg tcactgcgtt
     1021 tggggtaagc aacgctggat ccagtccggt ccgatcaacc gcgcgtgggg atgcatcatg
     1081 cggggcgtgt tcctctctcc gactctctcc tcaaggaaag agggcatctc cgcactacgt
     1141 ttcgtgcgca gtgcatctga agcggtcgtt cggccactga aggcgggtac ggagcccgcc
     1201 actacgtacc gtaaagattg caacctctgt ctgatcgcta ccgggtttcg tcggtgaagg
     1261 cgtaggcgat caggaggatg aggatcagga tgccctggat catctggcgc tcggcgtcgg
     1321 tcgtgcgcaa ggcggtcagc cagctgacga ggacgaccat gaccagcgcg ccgccgaggg
     1381 taccgatcag cttgccgcgg ccaccgagga tcgaggttcc gccgatcacg acgacggcga
     1441 tagactgcag cagatagggc gtgcccatgt cctggaagct gtatccggtc tggcccagca
     1501 gaatcagtcc gacgatgccg gccgagaggc tgcaaagccc gtaggtgacg acctgcgtca
     1561 agtcgaccgg caccccggcc aaaagcgagg cacgggcatt gacgccgatg gcgtagacgc
     1621 cgcggccgta gctggtcagg tggagcagaa tcatgcccag tcctgcaatg accagcgcgg
     1681 cgatggcggt gttcgggatc ttccaggtgc tgccgagcgc aagcttctcc agaaacggtg
     1741 aattgccgct gaccgggttg cccgagttgt agacacgtaa tgcgccatcg atgatcgccg
     1801 aggccgcgag ggtcatcacc agcggcggca ggcgcagcag gacgacgccg agcccgttga
     1861 gcatgccgac gccgaagacg atcgcgagcg tcatcaggat cccggcgacc tgtccgtgct
     1921 gcgagatcag gtttccgccg acgacaccgg ccagtccaat gaccgccgcg acggagagat
     1981 cgatgccgcc catcaggacg acgaaggtct ggccgattcc gacgatcgcg aggatcgagg
     2041 caaagcggat gaagcggaga ttggcgttga gcgtgcggta gccctcgatg tcgatcaacg
     2101 cgacagccca gaccactgcc aacacgacga aggtcagcag gacagggtcg agccggaaat
     2161 cgcgccagcg cgagcggcgc agctccagag cgcggtcggg ttgcagcggt tcgctggcca
     2221 tgatcagcgg ttctcctgca ggcggtaggt cgtgatcgcc ttgatgccga gcgcgatgat
     2281 caggacaagg ccctggacga tgcgctggta ggcagggtca aagccggaaa tggtcaggat
     2341 ctggatcagc aagccgaagg tcaacgcgcc ggcaaccgcg ccccgcaaaa gaccctgccc
     2401 accgaggaag ctgacgccgc cgaggaccgc ggcggtgatc gacatcagcg tgtacggcgg
     2461 gccgatcgtc gggtcgctcg tgccggtgta gccggccagc gcggacgccg caagcatgac
     2521 gaacacgccg gcggtgacgt aggcggcgat ccgggccagc cgcacgttga cgccgctgta
     2581 gaaggcgccg gtcggattgt cgccgaccgc gtagatgccg agaccaagcg gagatcgctt
     2641 gatgtatcgc catgcgagca gcgccagagc gaggataacg agcgtaaccg ggatgatccc
     2701 gaagatctgg tgtttgaaga aggtcgagta gctgcggggc aagtgtccac cagcttgggg
     2761 cagcgtgtag agcgccatgc ccatccagat gaacgaagtc gccagcgtca cgatgatcgc
     2821 cgggagccgg atgtaactga ccaccgagcc ggtgaagagt ccgattgcgg ctccggccag
     2881 caggaccagc agcacggagc ctaccttcga cgcgaaggtg tcctccattc gcgtcgcgag
     2941 cagggcggtg ccgagggtga gcattgcgcc gagcgagagg tcgatgccgc cggtgagcat
     3001 gacgatcgac tgtgccatcg atgccagcat caggggaagc aggtaggtcg agagattgtt
     3061 gatcgtgtgc cagccgatcg tgtcgcgcga ctggtagacg tagatcgcgg cgaagacagc
     3121 caggaccgcg aagatcggcc cgacatcttg ccaagtctgg ggcagccggt tgtaccgctc
     3181 cattagcgag agttttcgct gaaccgcctc agcgggcgcg tgctcgatcg tcggtgccgt
     3241 ccgtgccgtc ggggattcgg cggtaattgg caccggctcc tcctcaccgg cgcggcgacc
     3301 ggcgaccgtg gcgtggacga tgttctcctc ggtcacgtcg tcgccggcga gcatggcgac
     3361 cggccggtgc tcatacatga ccagcacgcg gtcactcagt ccgagcaact cgatcgtgtc
     3421 ggacgagatc aggatgatgg aggcgccggc gtcggcgagc tggcgcatca gcgtgtagat
     3481 ctcttgcttc gtaccgacat cgataccgcg cgtcggctcg gccagcagga agacatccgg
     3541 cgcgagcgga agccacttgg caatggcaac cttctgctga ttaccgcccg agagacgcag
     3601 gactggttgc ttcggcgagc gcaagcgaac ctgaagcttc cgcaccagat cggccaccag
     3661 gcgattctcg cggcgacggt cgagcagacc accgtgggtg atctgcggca ggttcggaag
     3721 cgtcaggttc tccttgaccg actgcggcag gagcagaccc tcggtcttgc gatcctcggg
     3781 cacgtacgcg atcttgcggc tcatggccga acgcgggctg cgcgagccga tcgcttgccc
     3841 gttgagcagg acctcgccgt cggtccggtg cagtccgaag agcgcggcga ggagtgcccg
     3901 ctggccctgg ccctgcaagc caccgagacc gaggatctcg ccccgatgga gctcgaagct
     3961 gacgtcccgg aagcggcgac ccgagcgcag gttgcgcacc tcgagcacag ccggttgggc
     4021 aaggacctcg tcgatggccg gcttggccgg gaagatcatc tccatcggcc ggccaaccat
     4081 gtactggacg agctgggcgg tcgatgtgtc ggcggtttcg acggtggcga cgtactcgcc
     4141 gttcttgagg accgtcatcc ggtcgctgat cgcgaaaacc tcgtcgagcc ggtgcgagac
     4201 gaagacgatc ccggtcccct cggcggccat acgccgcatc gcgccgaaga tcagcccgac
     4261 ctggtggctc tccagtgcgg aggttgcctc gtcgaggatc agcagcttcg gccgggtcat
     4321 cctcgccttg acgaactcga cgagctgccg ctcggcgagc gagaggtcgt cggtgcgcgc
     4381 ttcggggttg atctccaccc caagtgcgcg caattcctcg tccgactccc ggttgatccg
     4441 gcggtagtcg atttgcccgg aacgtgtcgg cgtgcggccg aggaagatgt tctcggcgac
     4501 ggtgaggtgc gggctgagac tcagctcctg gtaaacggtg gcgatacctt gctgcttggc
     4561 tgcgtgcgga tcgagccgat tgaagctgtg cccgttgacg acgatcgtgc cttcgtcggg
     4621 cgcatggacg ccggtgatca gcttcagaag cgtgctcttg ccggcgccgt tctcacccat
     4681 cagcgcgtgg atctcgccgg cgcgcacgcg gaagtcgaca ttgcgcagca ccacgttgcc
     4741 gctaaaggcc ttcgtgagcc cgtgcgtctc gagcacggtc atggtttcgg cccgcccggt
     4801 caaggcgcgc tgttcagcag gaacggtgtc gggcggcgac gcggcttcag ttgcggtccg
     4861 tgatgacgac atggctgcct gaccgagcgt agctgcgatt gcttcaggtt gaaacgagca
     4921 ggggatggag tgattgccat cccctgcaca tggtaccgag tggcgggccg ttttgcgtta
     4981 catggctggc gacggcgagg ccaccggcgt gcccatctcg gcagacagag cgtcggcaat
     5041 cagcttgtcg atctcctcgg gcgacggcac gtgcggaatc acgtaccagt catccgggtg
     5101 ctcgtccgat gcccagtcgc agacgttgtt gtcgtcgatg cgcggcagcg gcacaacgac
     5161 ctccttcggc acctgctcgc cggacttggc cttgaccgcg acatccaacg agatgaccgc
     5221 catgtagttg acctcggccg tggcgaaggc ggagaagccc tggtccttgg ccttgcacca
     5281 ctgcttgagg aacgcgttga agttctcgcc ggtcatcggg acgagcttca tgccggcctg
     5341 ggtgagagcc ttcatcgccg ccgaggcgtg cgcgccggcc tgacaccata ttcccgtgat
     5401 gctcgggttg gcggtcacaa ggctgttgag ctggaccagg gccggcgcct cgttgtactc
     5461 gatgttggcc gtgccgacga tcttgacgtc cgggttcttg tcgaagacct ccttggcgcc
     5521 ctgccagcgc tgctcggaaa ccgcgatacc ggacggtccg ttgatagcca ggatctcgcc
     5581 cttgccgccg agcttttcga cgagccactg ggcagtcatc cgcccccact cgatctgatc
     5641 ctcgtagacc ttgctggtga gctggttcgt ggtgacgagg ctgtcccagt tgacgacgat
     5701 tacgccggcg gccgtagcgg actcgacgac ctgatcaagg gcggtcgcgg agccggcgat
     5761 caggaggcag atgtcgtact tctggtcgat catcgtctgg atctggttga tctggg
//
LOCUS       DATAOZ010000520         2954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4943804, whole genome shotgun sequence.
ACCESSION   DATAOZ010000520 DATAOZ010000000
VERSION     DATAOZ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4943804"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VHR64_13220"
     CDS             complement(<1..287)
                     /locus_tag="VHR64_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303839.1"
                     /translation="MESAAVPVEAGGVAPGEKGLKSGALGYLSNLVIGVASTAPGYSL
                     AATLGFVTAVAGMGFHAPAIMIVSFIPMLLIAAAYSYMNKADPDCGTSFTWV"
     gene            complement(295..1170)
                     /locus_tag="VHR64_13225"
     CDS             complement(295..1170)
                     /locus_tag="VHR64_13225"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303840.1"
                     /translation="MEAHAATLPATPRESVIARAWKWLRHNAVLIFGCLAIVYMLLPI
                     GLIIVFSFNDPVGKFNFTWQGFTLEHWGNAFGIPELTDSLLLSLRLALFATVVSTVLG
                     TLVALAMVRYNFFGRRASNYLIIIPTATPEVVLGASLLSMFLIYGLSLGFTTLFIAHV
                     MFCISFVVIVVRSRLIGFDRSLEEAAQDLGASPLSTFLRVTLPLILPGVISAALLAFA
                     LSIDDFVVSNFTAGTSVTFPLYIYGAAQRGIPVEIYVIATILFSFTVLVMGFTVLQQR
                     RAARLAAIGPQQEGE"
     gene            complement(1170..2051)
                     /locus_tag="VHR64_13230"
     CDS             complement(1170..2051)
                     /locus_tag="VHR64_13230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303841.1"
                     /translation="MRDQRLVALRRRLIPYFLLGPGILWLILFFVVPMYFMGRLSLDS
                     GIFPEFHFSWEFSNYTDALKAYDTQFFRAFRYSAIATVLALLIAYPLAYAIAFRGGRW
                     KNAMLLAVIAPFFTTYLIRTLAWETILSDNGFVVHTLQSLGLISENGRVLATSGAVIA
                     GLTYNFLPFMILPIYASLERLDTSLVEAAKDLYASSRQAFLRVTLPLSMPGVVAGTLL
                     TFIPATGDYINAQFLGGAQNSMIGNVIQSLYLKQTDYPQAAALSFILMATMLAVVFVY
                     IRFAGSEALMGEEAERS"
     gene            complement(2085..>2954)
                     /locus_tag="VHR64_13235"
     CDS             complement(2085..>2954)
                     /locus_tag="VHR64_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3303842.1"
                     /translation="KMQPLLSKGQSGGRSIIVVTDWMAEKMHDLGYVQDLDKAGIPNV
                     EKNIRPDLRNVEFDPGRNYSIPWQSGMTGLIVNTRLAPNVHSINDLFDPQYKGKVEML
                     TELRDTVPLVMKADGIDPNKATQQQWLDTIDKLKSAVSSGQIRRFTGNDYTRDMANGD
                     VAAIIGWSGDAVQLQADNPAIEFRMPTEGCILWSDNMEIPVGAPNPTAAEAFMNYVYD
                     PKNQAPLAAYNSYVTPVDGVQDIFRTTNPDLVNDPLIFPTEDFTKNCSTQPNLTGQEE
                     QTVTRAFEGVLNG"
BASE COUNT          557 a          909 c          989 g          499 t
ORIGIN      
        1 acccaggtga agctcgtgcc gcagtcgggg tccgccttgt tcatgtagct gtaggcagcg
       61 gcgatcaaca gcatgggaat aaacgaaacg atcatgatcg ccggtgcgtg gaagcccatc
      121 cccgcgaccg ccgtcacgaa accgagcgtg gccgcaaggc tgtatccggg ggcggttgat
      181 gcgaccccaa tcaccaggtt cgacaggtag ccgagcgcgc ctgacttcag gcccttctcg
      241 ccaggggcga caccgccggc ctcgaccggt acggctgcgc tttccatgct gtccctactc
      301 cccttcctgt tgaggtccga tggcggccag ccgcgcggct cgccgctgct gaagaacggt
      361 gaatcccatc accaggaccg taaagctgaa cagaatcgtc gcgatcacgt agatctcgac
      421 cggaatcccc cgttgcgccg cgccatagat gtagaggggg aaggtcacgc tggtgccggc
      481 ggtgaagttg gagaccacga agtcgtcgat cgagagggcg aacgccagca gggcggcgct
      541 gatcaccccg ggcaaaatca gtggcagggt caccctgagg aaggtgctga gcggactggc
      601 gcccaggtcc tgagcggcct cttccaggct gcggtcgaag ccgatcaggc gcgagcggac
      661 gacgatcacg acgaagctga tgcagaacat cacgtgcgcg ataaaaagcg tcgtgaaccc
      721 gagcgagagc ccgtagatca agaacatcga gagcagcgag gcgccgagca ccacctcggg
      781 cgtcgccgtc gggatgatga tcaggtagtt cgatgcgcgg cggccgaaga agttgtagcg
      841 caccatcgcc agggcgacca gcgttccgag cacggtcgag accacggtcg cgaacaacgc
      901 cagccgcagg ctgagcagga gggaatctgt gagctcgggg atcccgaagg cgttgcccca
      961 gtgctccagc gtgaagccct gccaggtgaa gttgaacttg cccaccgggt cgttgaatga
     1021 gaagacgatg atcagaccga tcggaagcag catgtagacg atcgccaggc agccgaagat
     1081 gagcaccgcg ttgtggcgca gccacttcca ggcccgcgcg atgaccgatt cccgaggggt
     1141 ggcaggcagg gttgccgcgt gcgcctccat cagctcctct ccgcctcctc gcccatcagg
     1201 gcctccgacc cagcgaagcg gatgtagacg aacaccaccg ccagcatcgt cgccatcagg
     1261 atgaacgaca gcgccgcggc ctgcggatag tcggtctgtt tcaggtagag cgactggatg
     1321 acgttgccga tcatcgagtt ctgcgcgccg ccgaggaact gggcgttgat gtagtcgccg
     1381 gtcgccggga tgaaggtgag cagggtcccg gccaccacgc cgggcatcga gagcggcagc
     1441 gtcaccctca ggaatgcctg gcgcgaggag gcgtagaggt ccttcgccgc ctcgaccagg
     1501 ctggtgtcga gccgctccag gctggcgtag atcggcagga tcatgaacgg caggaagttg
     1561 taggtcagcc ccgcgatcac ggcgccgctg gtggccagga cccgcccgtt ctccgagatc
     1621 aggccgaggg actgcagcgt gtggacgacg aacccgttgt cggagaggat cgtctcccag
     1681 gcgagcgtcc ggatcaggta ggtggtgaag aagggggcga tcacggcaag caacatcgcg
     1741 ttcttccagc gcccgcccct gaacgcgatc gcgtaggcga gggggtacgc gatcagcagg
     1801 gcgaggacag tcgcgatcgc cgagtagcgg aacgccctga agaactgcgt gtcgtacgcc
     1861 ttgagcgcat cggtgtagtt cgagaactcc cagctgaagt ggaactcggg aaagatccca
     1921 ctatcgagcg agagccggcc catgaagtac atgggaacga cgaagaagag gatcagccag
     1981 aggatccccg ggcccagcag gaagtacggg atgagacggc ggcggagagc gaccaagcgc
     2041 tggtcacgca tcagtcgtct ccgccgcctc tcacctaact cccgctagcc gttcaggacg
     2101 ccctcgaacg ccctcgtcac cgtctgctcc tcctggcccg tcaggttggg ctgggtggag
     2161 cagttcttgg tgaagtcctc ggtcgggaag atcagaggat cgttcaccag gtccgggttg
     2221 gtggtccgga agatgtcctg gaccccgtcc accggggtga catagctgtt gtaggccgcg
     2281 agcggcgcct ggttcttcgg gtcgtagacg tagttcatga acgcctccgc cgccgtcgga
     2341 ttcggtgcgc cgaccgggat ctccatgttg tccgaccaga ggatgcagcc ctcggtcggc
     2401 atccggaact cgatcgccgg gttgtcggcc tgcagctgga ccgcatcacc ggaccagccg
     2461 atgatcgccg cgacgtcgcc gttcgccatg tcgcgggtgt agtcgttgcc ggtgaaccgg
     2521 cgaatctgtc ccgatgacac ggcggacttg agcttgtcga tcgtgtccag ccactgctgc
     2581 tgggtggcct tgttcggatc gatcccgtca gccttcatca ccagcggcac ggtgtcgcgc
     2641 agctcggtga gcatctcgac cttgcccttg tactgcgggt cgaacaggtc gttgatcgag
     2701 tgcacgttgg gagccagcct cgtgttgacg atcagcccgg tcatgccgct ctgccatggg
     2761 atcgagtagt tgcgccccgg gtcgaactcc acgttgcgga ggtcgggccg gatgttcttc
     2821 tcgacgttcg ggatcccggc cttgtccagg tcctggacat agccgaggtc gtgcatcttc
     2881 tccgccatcc agtcggtgac gacgatgatg ctccgcccgc ccgactggcc cttggacagc
     2941 aggggctgca tctt
//
LOCUS       DATAOZ010000521         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6256975, whole genome shotgun sequence.
ACCESSION   DATAOZ010000521 DATAOZ010000000
VERSION     DATAOZ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6256975"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VHR64_13240"
     CDS             complement(<1..811)
                     /locus_tag="VHR64_13240"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX3303843.1"
                     /translation="MAREAPEDPYAGLAPSELLAHGPFPDVQSEDNRQEDPKALHERA
                     LAAENAALAVEGVTNSSGGSAGTSASTVALATSGGFSGAYRSTGHSCSVTVIAGEGPT
                     MQRDSAWHGARHLEDLDAPDEVGKLAGERAAARLNPIKPKPGRYAVLFDPRVAGSLLG
                     HFIGAITGSSVARKSSFLQDKLGQQIFATGVRLIDDPLRPRGLRSRPFDGEGLPVTIS
                     ALVDDGVLKTWLADSASARQLGIQPTGHAVRGAGGPPGAGPSNLYLAPGSRS"
     gene            884..1069
                     /locus_tag="VHR64_13245"
     CDS             884..1069
                     /locus_tag="VHR64_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303844.1"
                     /translation="MSDEDAESDLFALRPIDMLELAKADLHPLRFRSDVDCVGAVRAR
                     CARLLNKRLGGFPRFIR"
     gene            complement(1085..>1880)
                     /gene="ubiA"
                     /locus_tag="VHR64_13250"
     CDS             complement(1085..>1880)
                     /gene="ubiA"
                     /locus_tag="VHR64_13250"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX3303845.1"
                     /translation="LLYWPCAWSVALAGVDGRWDLLLWLFLGAFAMRSAGCVYHDIVD
                     RDLDRQVERTRLRPLASERVSLRSAWMLIALLCAIGLIVLVQLNTTAAVISLVSVAPV
                     AAYPFMKRITWWPQAWLGLVFSWGALVGWPAVRGTIDHPAVLLWFGSIAWVVGYDTLY
                     AIQDIEDDALVGVKSSARRLGDKAPQGVALFYLLAIALWGSAIWTVRPDWLALAALAP
                     AALHLANQALRADPKDGEGALRLFRSNRTTGLLIFLGMLVVGLSSR"
BASE COUNT          344 a          638 c          617 g          281 t
ORIGIN      
        1 ggctgcgcga tccgggggcg aggtaaagat tgctcgggcc cgcgcccggc gggccaccgg
       61 ctcctcgcac ggcatggccg gtgggctgga ttccgagctg ccgggccgat gcgctgtcgg
      121 cgagccaggt cttcaggact ccgtcgtcga ccagcgccga gatcgtcacc ggcaatcctt
      181 cgccgtcgaa aggccgtgac ctcagtcccc gcggacgaag cggatcgtcg atgagccgca
      241 cgccggtcgc gaagatctgt tgcccaagct tgtcctgcag aaaggaggat ttgcgggcca
      301 cggacgaccc ggtgatcgca ccgatgaaat gccccagcag cgaacccgcg acacgcggat
      361 cgaacaggac ggcgtagcgg ccgggcttcg gcttgatcgg gttgagccgg gccgcggcgc
      421 gttcccccgc gagcttcccc acttcgtcgg gcgcatccag atcttcgaga tgccgggccc
      481 cgtgccaagc gctgtcgcgc tgcatcgtcg ggccttcacc cgcgatgacc gtcacggagc
      541 agctgtgacc ggtcgaccga tacgcgccgc tgaagccacc cgaagtcgca agcgctaccg
      601 tgctcgcgga cgtgccggcc gatccgccac tcgaattggt gacgccttcg acggccagcg
      661 cggcgttctc ggctgcgagt gcccgttcgt gcaaagcctt cggatcttcc tgacggttat
      721 cctccgactg gacgtcggga aacggtccgt gcgcaagcag ctcggacgga gcaaggccgg
      781 catacgggtc ttccggggcc tcccgagcca ttgcgacgcc ccgattgacg agctccgcca
      841 gcgcgtccgg ggaaaagtcg gacgatgcta cgcttgcact ttggtgtccg acgaagacgc
      901 ggagtccgac ctcttcgccc tccgaccgat cgacatgctc gagctcgcca aggcggacct
      961 gcaccccctg cgattccgat ccgacgtaga ttgcgtcggc gctgtccgcg cccgctgcgc
     1021 gcgccttctc aacaagcgat tgggcggttt cccgcgcttt atccgctgac agcattggaa
     1081 gcgcctagcg gctggaaagg ccgacgacca gcattcccag gaagatcagc aggcccgtag
     1141 tgcgattcga ccggaacagg cgaagcgcgc cttcgccatc cttcggatcg gcccgaagcg
     1201 cctggttggc gaggtggaga gcggcggggg cgagcgcggc gagggcaagc cagtccggcc
     1261 tgacggtcca gatggccgaa ccccaaagcg cgatggccaa tagatagaag agggcaacac
     1321 cctgcggggc cttgtcgccc agccgccgcg cggaggactt cacgccgacc aatgcgtcgt
     1381 cctcgatgtc ctggattgca tagagcgtgt cgtacccgac cacccaggcg atgctcccga
     1441 accagagcag cactgccgga tggtcgatcg ttccacgtac cgccggccag ccgaccaaag
     1501 ctccccaaga gaagacgaga ccgagccagg cttgcggcca ccaggtgatg cgcttcatga
     1561 aggggtaggc tgcaaccggt gcgacgctga ccagcgaaat gacggcagcg gtcgtgttga
     1621 gttgaaccag gacaatcaag ccgatcgcac agagtagcgc gatcagcatc catgcggacc
     1681 gcaacgatac ccgttcgctc gcaaggggac gcaggcgcgt tcgctcgacc tgccggtcga
     1741 ggtcgcggtc gacgatatcg tggtagacgc agcccgcgct ccgcatcgcg aaagcgccga
     1801 ggaacagcca caggagtagg tcccaacgcc cgtccacccc cgcaagcgcg acgctccacg
     1861 cgcagggcca gtatagcagc
//
LOCUS       DATAOZ010000522         5819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6257073, whole genome shotgun sequence.
ACCESSION   DATAOZ010000522 DATAOZ010000000
VERSION     DATAOZ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6257073"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VHR64_13255"
     CDS             <1..305
                     /locus_tag="VHR64_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303846.1"
                     /translation="VTWRLTESVRNPENYQSYLKTLAQKNVPILRRFGLLDSFVLRMS
                     DDTVQVVNVFEDRDGAESAWREINGSLAPAIEGHLEFLNRISARADDLPLLMDFGN"
     gene            complement(332..1093)
                     /locus_tag="VHR64_13260"
     CDS             complement(332..1093)
                     /locus_tag="VHR64_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303847.1"
                     /translation="MSEHSRPGGNPASGQPLRFSVPDLASLLGVSEQAVLKQIERGEL
                     LAIQEEDGWMVYVDLPEVAQPAATDNPGPGSSGAESPHDEVLSITHAVEDRARDASER
                     PPALPVPVAIPVSPVDLTLLINLVTDLTKRNAELLESTATWRERVQHLETELQRVMIA
                     ASTPDETPESELPAIGDAAVESSQLEEERAARIAAEAEVARLHVELREVRQLQNVLQN
                     AFQDPAARTAAGWRNATSEPAQGKPSWWRRIVGGS"
     gene            complement(1175..1564)
                     /locus_tag="VHR64_13265"
     CDS             complement(1175..1564)
                     /locus_tag="VHR64_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303848.1"
                     /translation="MEKRGTIRIQTLIAEYLTQLADWRRMRYQDDLRDTRNLRSAEGI
                     EALAAYIRALPDNDARMTELDRLWRRGEQVEVGQQAAYEIGRFRFFLPETELDAFLDE
                     VVDLARSDANEHGQFGGQQAPGDNPWG"
     gene            1591..2826
                     /locus_tag="VHR64_13270"
     CDS             1591..2826
                     /locus_tag="VHR64_13270"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HEX3303849.1"
                     /translation="MSEQLIHPDLIDQRVQDLSRFGKVGETGVTRTVYSPEWIAAQDH
                     LETWMRAAGLETRRDAVGNLWGVALGTEPGPSIVSGSHVDSQAPGGRFDGALGIIAAL
                     TATEALLKRHGQPKRTIEVVSLCEEEGSRFSTAGFWGSRAILGLITPEDLETTISFDG
                     EKIGDVMRGIDLDPARIPEAKRSDIDTFIELHIEQGPVLELERLPVGIVTGITGYRHY
                     VVEVSGWANHAGAFPMKYRRDPMAAAAEMISGVIQNAVDMGEPAVTTVGRLAIEPNYP
                     AIIPERVTFMIDARHPDPVLREQLYEKHEKLIYEIADRRRLNVDVHINKDLAPRPCDP
                     GIVDLLDQAATDLGIAHKQMSSGAVHDTQRFSQIAKTAMIFVQSKDGRSHTPEEFTST
                     EHATLGTEVLAEGLSRLAY"
     gene            3007..4143
                     /gene="solA"
                     /locus_tag="VHR64_13275"
     CDS             3007..4143
                     /gene="solA"
                     /locus_tag="VHR64_13275"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEX3303850.1"
                     /translation="MTEKFDVIVAGVGGMGAAACYHLAKRGKRVLGLERYGIPHSMGS
                     SHGVNRIIRLAYYEDPSYVPLLRRAFELWRELGRDFGEQVLYVTGSIDTGPVDEFVFR
                     ESRRSCDLHELPHELLSAHELMRRYPAFSVPDDFEAVLQPDGGFVASERAIVAHVNGA
                     MAYGAVIRGHEPMLSWTTTSSGVTVETSKGLYEADQLIVSTGAWIGDHIPALSGISEP
                     ERQVLGWFQPAHPEHFSLESLPVFNLQADAHERYYGFPIWGIPGFKFGKYHHREERID
                     PDDWDRQPNQADEDVLRDGVRRFFPDADGPVMSMASCIFTNTPDEHFIIDRLKPGSPV
                     IVASPCSGHGYKFASVIGEILADLAINDRTDHNVNLFRLDRFQR"
     gene            complement(4423..4962)
                     /locus_tag="VHR64_13280"
     CDS             complement(4423..4962)
                     /locus_tag="VHR64_13280"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX3303851.1"
                     /translation="MPPQRRVYLLPHDPAWAEQATQHASVVTKILGPLLVRWEHIGST
                     SIPGIHAKPVIDLMPLVTAIEAVDPLQGEFESAGFCWYGEYGLPGRRYVNRDNPETGD
                     RLTNVHIYAVDDTEVERHLAFRDYLRAHLDIAREYEAVKLDCAAKHPADIYGYMDCKD
                     ATCKQIESDAVAWYRSMRA"
     gene            complement(4962..>5819)
                     /locus_tag="VHR64_13285"
     CDS             complement(4962..>5819)
                     /locus_tag="VHR64_13285"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX3303852.1"
                     /translation="ADMGLWDSVWLYDHFHTVPTPELMTTFECWTATATLARDTERVK
                     VGQMVGCNGYRNPALYAKIASTVDVASHHRLYAGIGAGWYEHEWKAYGYEWTETPTRM
                     GRFRETVQIIHQMWTEDYPQFSGRHYTIDKPINEPKDAGKGKIPLWIGGGGEQVTLKL
                     VAQYGDACNFSGDSATIRHKLDVLRGHCEKVGRDYDEIIKSSQIRIHLIEPGEDPEKA
                     SASYRGTHYTWERYQSEMLVGTIDQVRSEVERHIEDGIDYMIIYIAKAAYEPDLVQYV
                     SDEVVRKYS"
BASE COUNT         1049 a         1890 c         1794 g         1086 t
ORIGIN      
        1 tcgtcacctg gcggctgacc gaatccgttc gcaaccccga gaattaccag agttacctga
       61 aaactctggc ccagaagaac gtccccattc ttcgccgatt tggcctgctg gatagcttcg
      121 tcctgcggat gagcgatgac acggtccagg tggtcaatgt cttcgaggac agggacggcg
      181 ctgaatcggc ctggagggag atcaatggca gcctcgcacc tgcgatcgag ggccatctcg
      241 agttcttgaa tcgcatttca gcccgggccg acgatctccc gctgctgatg gatttcggta
      301 actgaacgca acgtccgaac ggtgaggtcg atcaggaacc acccactatc cggcgccacc
      361 atgacggctt gccttgcgct ggctccgatg tcgcgttgcg ccatcccgcc gccgtccgtg
      421 ccgctggatc ctggaaggca ttctgaagga cgttctggag ctgcctgacc tctcgcagct
      481 cgacgtgcaa tctggcaacc tccgcctcgg ccgcaatgcg cgctgctcgc tcctcctcga
      541 gttgcgatga ctcgaccgcc gcgtcgccaa ttgcaggcag ttccgactcc ggagtttcat
      601 ccggcgtgga tgcagcgatc atcacgcgct ggagctcggt ctcgagatgc tgaacacgct
      661 cccgccaggt cgcggtcgat tccagcagct cggcattgcg cttggtcagg tcggtgacga
      721 ggttgatcag cagcgtcaga tcgaccggcg agacgggaat cgccactgga accggcaacg
      781 ccggtggacg ctccgaagcg tcccgggctc gatcttcgac cgcgtgtgtg atcgaaagga
      841 cctcgtcgtg cggggattct gctccggatg acccaggacc aggattgtcg gttgcggccg
      901 gctgtgcgac ttccggcagg tcgacgtaga ccatccagcc gtcctcctcc tgaatcgcga
      961 ggagttcccc tcgctcgatc tgtttcagaa cggcctgctc gctgacgcca agcaacgacg
     1021 ccaggtcggg aaccgaaaag cggagcggct ggccggacgc cggattgccg cccggacggc
     1081 tgtgctcgct catcggcgga ccgcgccagg cgacccgagg tccatacttc ccgattctga
     1141 acccggaatg cgcgcagctc gctccatcag tcgatcaccc ccacgggttg tcaccgggcg
     1201 cctgctggcc cccgaattgt ccgtgctcgt ttgcgtcgga ccgggcgaga tcgaccactt
     1261 cgtcgaggaa cgcgtccagc tcagtttcgg gcaggaagaa acggaaccgg ccaatctcgt
     1321 acgcggcctg ctgccccact tcaacctgct cgccacgtcg ccagagccgg tccaactcag
     1381 tcatgcgcgc gtcattatcg ggcagtgcgc gaatgtatgc tgccagcgcc tcgataccct
     1441 ccgcgctgcg cagattccgg gtgtcccgca agtcgtcctg gtaccgcatc cggcgccaat
     1501 cggcgagctg ggtgagatac tccgcgatga gcgtttgaat ccggatggtg cctcgcttct
     1561 ccatgcccgg atgatatacg gggatgcgga atgtcggaac agttaattca ccctgacctt
     1621 atcgaccaac gggtccagga cctgtcgcgc ttcggcaagg tcggcgagac cggcgtcacg
     1681 cgcaccgtct attcgccgga atggatcgcc gcccaggatc acctcgagac ctggatgcgc
     1741 gctgccggat tggagacgcg ccgggatgcg gtcggcaatc tctggggagt cgccctgggc
     1801 accgaacctg gaccatcaat cgtcagcggc tcgcacgtcg attcacaagc gcccggcggt
     1861 cgcttcgacg gcgcgctcgg catcatcgcc gccctgaccg caacggaagc gctgctgaag
     1921 cggcacggtc aacccaagcg gacgatcgag gtcgtctcgc tctgtgagga agagggcagc
     1981 cgcttctcaa cggccgggtt ctggggatca cgcgcgatcc tcggcttgat cacgcccgag
     2041 gatctcgaga ccacgatcag cttcgacggc gagaagatcg gcgacgtcat gcgcggaatc
     2101 gacctcgatc cggcccgcat cccggaggcg aagcgctccg acattgacac cttcatcgag
     2161 ctccacatcg agcagggtcc ggtgcttgag cttgagcggc ttccggtcgg aatcgtgacc
     2221 gggatcaccg gctaccgcca ctacgtcgtc gaggtgtcgg gctgggccaa ccacgccggt
     2281 gcctttccga tgaagtaccg gcgggatccg atggcggcgg cagccgagat gatctccggc
     2341 gtgattcaga acgcggtcga catgggcgaa ccagccgtga ccaccgtcgg ccggctggcg
     2401 atcgaaccaa actacccggc gatcatcccg gagcgcgtca cgttcatgat cgacgcgcgc
     2461 catccggatc ctgtgctccg ggaacagctc tatgagaagc acgagaagct gatctatgag
     2521 attgccgacc ggcgcaggct gaatgtcgac gtccacatca acaaggacct cgcaccgcgg
     2581 ccgtgcgatc ccggcatcgt cgatctgctc gatcaggcag cgaccgacct tggcatagcg
     2641 cacaagcaga tgagctcggg agcggtccac gacacccagc gcttctcgca gattgccaaa
     2701 acggcgatga tcttcgtcca gagcaaggac ggccgcagcc acacgccgga ggagttcacc
     2761 agcaccgagc acgcgaccct cgggaccgaa gtgctcgccg aagggttgag tcgactcgca
     2821 tattgacgcg ttaccaggcg tagcgcgggg ttaccatgcc ccgctggtct tatcgggaaa
     2881 acacctgtct gaggcggacc aactctccgg caagaccatc atcacccttc gggcgatcgc
     2941 cgggcgtgga aacccggcac tacatcactt cgaaaccaag aaacgcggga accggggaga
     3001 ggagcaatga ccgagaagtt cgatgtgatc gtggccgggg tcggcggcat gggtgctgcc
     3061 gcgtgctacc acctcgcgaa gcgcggcaag cgagtactgg ggcttgagcg gtacggtatc
     3121 ccgcattcga tgggttcctc gcacggggta aaccggatca tccgcctcgc ctactacgag
     3181 gacccgtcct acgtgccgct gctccggcgc gccttcgagc tctggcgcga gttgggccgc
     3241 gacttcggcg agcaggtgct ctacgtgacc ggctcgatcg acacaggacc ggtcgacgag
     3301 ttcgtcttcc gcgaatccag gcgctcctgc gacctccatg agcttccgca tgaactgctt
     3361 tcggcgcatg agctaatgcg ccgctacccc gccttctcgg tgcccgatga cttcgaggcg
     3421 gtgctgcagc cggacggcgg gtttgtcgca tcagagcgtg caattgtcgc gcacgtcaat
     3481 ggcgcgatgg cgtatggcgc cgtcattcgc ggccacgagc cgatgctgtc gtggacgaca
     3541 acgtcctcgg gcgtgaccgt cgagacgtcg aaggggctct acgaggccga ccagctcatc
     3601 gtcagcactg gcgcctggat cggcgaccac attcctgccc tgtccggaat cagcgaaccg
     3661 gagcggcagg ttctcggctg gttccagccc gcacatccgg agcacttctc gctcgaatcg
     3721 ctgccggtct tcaacctgca agcggatgcg cacgagcgct actacggctt ccccatctgg
     3781 ggcattccgg gcttcaagtt cggcaagtat caccaccgcg aggagcggat cgatcccgac
     3841 gactgggacc ggcaaccgaa ccaggccgac gaggatgtcc tgcgggacgg tgtgcgccgc
     3901 ttcttcccgg acgccgatgg gccggtcatg tcgatggcgt cgtgcatctt cactaacacg
     3961 ccggatgagc acttcatcat cgaccgcctc aagcctggca gtcccgtcat cgtcgcctcg
     4021 ccatgctcag gccacggcta caagttcgcg tcggtcatcg gcgagatcct ggccgacctc
     4081 gcgatcaacg accgcaccga ccacaacgtc aacctgttcc gcctcgaccg cttccaacgc
     4141 tgaccgcagc cctaccagct atctgacggc ggcagatttc gtggacaccg ggcaggttgc
     4201 gcaccgtgaa catcatgcat atcttgcaca agttgctcaa catgctcagc tatcacctgt
     4261 cgcggtagat cgctcctcaa agtggatcct ggaatgactg cgtatcattc gggaccatgc
     4321 gcaagatgtg caacttgagc acggtgagca tcttgtgcat ggtgctcaca gcgaatgtca
     4381 agatgagcaa cctgcgcaac gtggccgtgc gtggattgtt tcttacgcgc gcatcgaccg
     4441 gtaccaggcg acggcgtcgg attcgatttg cttgcaggtg gcgtctttgc agtccatgta
     4501 gccgtagatg tcggcaggat gcttggccgc gcaatcgagt ttcaccgcct cgtactcgcg
     4561 ggcaatgtcc aggtgagcgc gcaagtagtc ccggaaggcg aggtgccgct cgacttccgt
     4621 gtcatcgacc gcgtagatgt ggacgttcgt gagtcgatcg ccggtctccg ggttgtcccg
     4681 gttcacgtag cgccgtcccg gcaggccgta ctcgccgtac cagcagaagc cagcggattc
     4741 aaattcgccc tggagtggat cgaccgcctc gatcgcggtg accagcggca tcagatcgat
     4801 caccggcttg gcgtggatcc ccggaatcga cgtcgaccca atatgctccc agcgcaccag
     4861 caacggaccc agaatcttgg taaccacgct ggcgtgctgc gttgcctgct cggcccacgc
     4921 tggatcgtgc ggcagcagat agacccggcg ctgaggcggc actagctgta cttccggacg
     4981 acctcgtccg agacgtactg gacaagatcg ggctcgtagg cggccttggc gatgtaaatg
     5041 atcatgtagt cgatgccgtc ctcgatgtgc cgctcgacct cgctccggac ctggtcgatc
     5101 gtcccgacca gcatctcgct ctggtatcgc tcccaggtgt aatgggtgcc gcggtatgag
     5161 gcggaggcct tctcaggatc ctcgcccggc tcgatcaagt ggatccggat ctggctggac
     5221 ttgatgatct cgtcatagtc gcggccgacc ttctcgcagt gaccccgcag cacgtccagc
     5281 ttgtgccgga tcgtggcgct gtcgccgctg aagttgcagg catcgccata ctgggcgacc
     5341 agcttcagcg tgacctgctc gccacctccc ccgatccaca acggaatctt gcctttgccg
     5401 gcatccttcg gctcattgat cggcttatcg atcgtgtagt ggcgacccga gaactgggga
     5461 tagtcctcgg tccacatctg gtggatgatc tggaccgtct cccggaaccg gcccatccgg
     5521 gtcggggtct ccgtccactc gtagccgtag gctttccact cgtgctcgta ccagcccgcc
     5581 ccgatgccgg catagaggcg atgatgagat gcgacatcga ccgtgctggc gatcttggcg
     5641 tagagggcgg gattgcggta gccgttgcag ccgaccatct ggccgacctt gacccgctcg
     5701 gtatcgcggg ccagggtcgc ggttgcggtc cagcactcga aggtcgtcat cagctccggc
     5761 gtcgggacgg tgtggaaatg gtcgtagagc caaaccgagt cccagagccc catgtcggc
//
LOCUS       DATAOZ010000523         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4682884, whole genome shotgun sequence.
ACCESSION   DATAOZ010000523 DATAOZ010000000
VERSION     DATAOZ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4682884"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..407)
                     /locus_tag="VHR64_13290"
     CDS             complement(6..407)
                     /locus_tag="VHR64_13290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX3303853.1"
                     /translation="MAAIPEKYLDLLEQKKAFASLATTMPDGSPQVTPVWFDYKDGIL
                     RVNTAKGRVKARNLKPGAAVALAIMDPDNPYRYIQVRGHVRRVTEEGAAAHIDSLAKK
                     YLGQDKYPFGQPGEVRLTCDIEPMSASGVGS"
     gene            complement(556..1827)
                     /locus_tag="VHR64_13295"
     CDS             complement(556..1827)
                     /locus_tag="VHR64_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX3303854.1"
                     /translation="MNYVHSDLRREAAPDPAVEHEAPPADVPEKRGGRRGWLLGLAVL
                     FVLAAALAFGGYRSYSQSARAAAATQQRLDAVPTLRVQAVQASGEYVAVTLPATTAAF
                     SVANIFARASGYIDKRNVDIGDHVKEGQLLVQIVAPELDHQIAQAKATLAQLEATVQQ
                     TQANKELARVTWGRDSPLVDKGWVTPQQGTIDVQTLKAQEAAVGVAEANAAAQRAQIQ
                     VLTQQKAYQRVVAPFDGVITQRNVDVGTLVQADAVSGTFMFTIMQSNVIRTQVFVPQD
                     QAFGLAPGVDAVVHVPEIPGRTFPGTVTRIADALQPGTRTLLTEVDIPNPDGALTPGI
                     YCTIELHSPRKTPSLVVSADAIIFNADGPQVAVVEDGVVHLRKISIARDLGKQIEVRD
                     GVRGGDQVILNPPVDLVEGKKVQVRPSDAKG"
     gene            complement(1824..>2079)
                     /locus_tag="VHR64_13300"
     CDS             complement(1824..>2079)
                     /locus_tag="VHR64_13300"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX3303855.1"
                     /translation="SAIEAGHTRIRTVLMTAAAMIVGMIPMAIGGAGEEQNAALARAV
                     IGGLLFATPTTLLVVPYLFAMLRKRNDGKTAQGVFSEDIR"
BASE COUNT          348 a          694 c          652 g          385 t
ORIGIN      
        1 ccgcgtcacg agcccacgcc ggacgccgac atcggctcga tgtcacaggt caggcggact
       61 tcaccgggct gaccgaacgg gtacttgtcc tgcccgagat acttctttgc cagcgaatcg
      121 atatgggcag ccgcgccctc ctccgtgaca cgcctgacgt gcccacgcac ctggatgtag
      181 cggtagggat tgtccggatc catgatcgcg agggccacgg cggcacctgg tttcaggttg
      241 cgtgccttga cgcgcccttt cgcggtgttc acccgcagga tgccgtcctt gtagtcaaac
      301 cataccggcg tgacctgggg cgatccgtcc ggcatggtcg tggcaagact tgcgaacgcc
      361 ttcttctgct caagcaggtc gagatacttt tccggaatcg ctgccatggc ctgggtccct
      421 cgctgcggcc gccttccgaa cggcgtatca gggaattctt cagccgaaca gggggacctc
      481 gtcgagcctg gccggcacgg agtcggcacg tcttgacgtc ccggatgtca cgccaaccgt
      541 ctcgtgaagc ggatgttacc ccttggcgtc ggatgggcga acttgcacct tcttgccctc
      601 gaccaggtcg accggcgggt tgaggataac ctgatcgccg cccctgacgc cgtcgcgcac
      661 ctcgatctgt tttcccagat cacgcgcgat cgatattttc cgcaggtgga cgacgccatc
      721 ttcgaccacg gcgacctgtg gtccatcagc gttgaaaatg atcgcgtcgg ccgacaccac
      781 gaggctcggg gtcttgcggg gactgtgaag ctcgatggtg cagtagattc ctggcgtcaa
      841 tgccccgtcc ggattgggaa tgtcgacttc cgtgagcaac gttcgcgtac cgggctgcag
      901 cgcatcggcg atacgcgtca ccgtgcccgg gaaagtacga ccagggattt cgggcacgtg
      961 tacgaccgcg tccacccccg gcgccaatcc gaaggcctga tcctgcggca caaacacctg
     1021 agtgcggatt acgttgctct gcatgatggt gaacatgaag gtgccgctga ccgcgtcggc
     1081 ctgcaccagc gtaccgacgt cgacgttgcg ctgggtgatc acgccatcga acggagccac
     1141 gacacgttga taggcctttt gctgggtgag gacctggatc tgcgcccgct gcgccgctgc
     1201 attggcttcc gccacgccca cggccgcctc ctgcgccttc agggtctgga cgtcgatggt
     1261 gccctgctgc ggggtcaccc agcccttgtc gaccagtgga ctgtcgcgtc cccaggtcac
     1321 ccgcgcgagc tccttgttcg cctgcgtctg ctgcaccgtc gcctcgagct gcgcaagggt
     1381 cgccttggct tgggcgatct ggtggtcgag ctccggggcg acgatctgca ccagcaactg
     1441 cccctccttc acgtgatcgc cgatatcgac gttgcgcttg tcgatatatc cgctggcgcg
     1501 agcaaatatg ttggcgacag aaaacgcggc ggtcgtggcc ggcaacgtga ccgcgacata
     1561 ctccccgctc gcctgcaccg cctgaacccg caaggtcggg acggcatcga gacgctgctg
     1621 ggtggccgcc gcagcgcgcg cactctggga atatgaccgg taccctccga aggccagggc
     1681 cgcggcgagc acgaacagca cggcgagccc aagcagccag ccacgccgtc cgccccgctt
     1741 ctccggcaca tcggcgggtg gcgcttcgtg ttcgacagca ggatcgggag cggcctcgcg
     1801 gcggaggtcc gagtgaacat agttcatctg atgtcctcac tgaacacgcc ctgagccgtc
     1861 ttgccgtcgt tgcgcttccg cagcatggca aacaggtacg gaaccaccag gagggtcgta
     1921 ggagtggcga acaacagacc gccgatgacg gcgcgagcta gggcggcatt ctgctcctcg
     1981 cccgcgccgc cgatcgccat cggaatcatg ccaacgatca tcgcagccgc cgtcatcagc
     2041 acggtccgga tacgggtgtg ccccgcctcg atggcggac
//
LOCUS       DATAOZ010000524        10273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3893381, whole genome shotgun sequence.
ACCESSION   DATAOZ010000524 DATAOZ010000000
VERSION     DATAOZ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3893381"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VHR64_13305"
     CDS             <1..1476
                     /locus_tag="VHR64_13305"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX3303856.1"
                     /translation="RYLLDRVLRTFLLQSSPVSLGTPLRVDAPTLESYCRERMTELGL
                     APVDAELKVDGESILVTQDAGGLVISVDRLQKDLVRQLSGFTTPRIDLTATYSPAMIR
                     ANEIESEISSLSSVLQQPMRLYTDTDQWDIPPSQMAANLKIDQSNGRPTLMIDDAAIT
                     GLVDRIANEIDQPVSNGSVDDSGIYARIIAPHDGVTVDRATLNQRIHDAITTGLNEVE
                     IPVTTTPSNGDLKPLLTKYGITDLLATGSSSFVGSDPGRTTNVRRAAQLIDGTLVAPG
                     EVFSFNQALGSIVDVGGFVPAGATEGGIPGTAVGGGVCQVSTSVFRAALRAGLPITEW
                     YPHAYRSMYYEQDSWAPGFDASIQQPDEDPLNGPDLKFTNVTDGWLLVRATASSTGEL
                     KVSLFGTATGFDIVISDPNYGAIVPADQAPVEEVDDSLPSGTMDLWQPARDGVTMSFH
                     RTVYAADGTLLIDEDFVSYYQPQGPVYRVSPDKAGSATAGY"
     gene            1505..2503
                     /locus_tag="VHR64_13310"
     CDS             1505..2503
                     /locus_tag="VHR64_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303857.1"
                     /translation="MPRTLVALAILWSMLATSANATPTSLPSPPPQPASGPGGSDYAF
                     EQVHAEHVGTAPTGYWLFSPLRDASSDPLPVVVFLHGFSAVDPDGYRAWIDHIVRRGA
                     ILIYPDYQTDKLTQTSSADYQPNAIAGIMAAVSHLGSGSVGPVDLTAIAIVGHSLGGV
                     LALNIAAVAREHELPAVAAVLSVEPGGCAECGGISRLIGLPFADLSGVGPETRVIVMV
                     GEDDQVVSDHGARTAWNLLTNVPADHRDFVRMHSDHRGSHALIADHYAPLAASSGWDV
                     DAMDWFGTWKLFDLLTGCAFHDLGCSTSFGGSEIETNMGEWSDGTTVIPLSVTDTP"
     gene            2503..3393
                     /locus_tag="VHR64_13315"
     CDS             2503..3393
                     /locus_tag="VHR64_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303858.1"
                     /translation="MRTISIEATAGYSLAQTAAPVAWGGGRWPNQDWIGGRLITVSVE
                     QSSSCLRIVHQPGPESTVLELETDRPELDHQDWADRVLGVRRIAPAIGDPVVRSIAER
                     FPGMRPYSNGSLFAGIVTAIVGQSISVQAAAVTERKLAALFAAPITIGERHYWPEPSV
                     RQLADASPHLVRQSGVTQRRADAIVHIARLATENQLLAPGVDPDDEQAVFAAMLRLPQ
                     VGRWTAESVLLWGLGSDDAYPAGDVALLRAARLAYARPEMTHGEMDTLSECWTPGRSW
                     AARWLWLNLFGPAPQPSPPG"
     gene            3474..4112
                     /locus_tag="VHR64_13320"
     CDS             3474..4112
                     /locus_tag="VHR64_13320"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX3303859.1"
                     /translation="MPHFDLEGFIKAVSYFGVAGVVFAETGLFFGIFLPGDSLLFTAG
                     FLASQGHLNIFLLCLVCFLAAVVGDSVGYYIGDRMGRRLFTKPQSRVFKPSNLIKAQA
                     FFDKHGGKAIILGRFMPIVRTMVPMVAGAGTMQYRRFFTYNVVGAFLWGVCIPMAGYF
                     LGSTIPGVDKYLLPIILVIIVVSIAPSAYHIWKENGELIKAEARRRLTERRT"
     gene            complement(4109..4888)
                     /locus_tag="VHR64_13325"
     CDS             complement(4109..4888)
                     /locus_tag="VHR64_13325"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX3303860.1"
                     /translation="MPDLMQSAWNALDLAWWQAIAALCAVTLGATISGLTGFGFGLVI
                     VPFMLMLFPPPAVVVLTSSLAIASGLPILIEDKDKIRVRIVSPLMVPALLGLLIGVRI
                     LTGVDTRYIKLAAGFIVVVFAIMVARGFVIPGIRSRIAPVVAGLTSGILGTSTGMSGP
                     PVVLFLTDRAPEPKVFRASITFYFSAMNLVGIFLVVRTGAVGGREFGIAAALLPVALI
                     GRRIGQRLHDRVDQAQFRRITLALLILTGTSGMLTALAGLF"
     gene            complement(4963..6579)
                     /locus_tag="VHR64_13330"
     CDS             complement(4963..6579)
                     /locus_tag="VHR64_13330"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX3303861.1"
                     /translation="MLIRNGSVWTGNESQPTAEAVAVRDGRIVAVGTESDARARLGRD
                     AEEIDAGGRTVLPGFLDVHNHYLATAEQLASVDLRYPAVASISDLTRAIAEAAENTPD
                     GRWIRGFSLDWAKFPDGRLPTRDDLDAATQVHPVIVYHVSGHHALVNTAAMLDRGILE
                     SAADPSGGKFVRDEGGRLTGLCLDSATSKILPVELDIGCHGPNFHTILPGQDTVALLD
                     AASHEYLKVGLTTICDPQVTRREMKGYREAHRQGVLRLRVIMMPLSNHLDDFISIGMA
                     GPFGDDQLMVGPMKLYSDGTLIGGTAWFTEPYGEQGQFTGSTYWKPNELAELVTRAHA
                     AGWQVGIHTGGDAAMEMTINAIEAGLRANPRLDTRHRIEHCFYPTPQQIERMASLGII
                     PVNQPNMLCDCGDEFVSRLGQRAHRLEPMREELDAGIKPVLSSDAFVTSFNPLHTIAN
                     ALDRKTRQGQPIGEEQRLTLEEALRAHTIDAAVSIRMEDRLGSLEVGKLADIAILDGD
                     LNAAAPAEIRDIPIWRTILGGEVLWSAPPA"
     gene            complement(6597..7967)
                     /locus_tag="VHR64_13335"
     CDS             complement(6597..7967)
                     /locus_tag="VHR64_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX3303862.1"
                     /translation="MSDTTKFLLTEDDLARDWYNIAADLPSPVPPPLHPGTLQPIGPA
                     DLAPIFPMEVIKQEVSQDRWIPIPDEVRDAYRLWRPTPLHRAHRLEKELGLPERVRIF
                     YKYEGVSPSGSHKPNTAVPQAFYNKQEGVKRIATETGAGQWGSSLALACQMFDLELKV
                     YMVKISYAQKPYRRALMETWGATCVPSPSPDTNAGRAILAQDPESTGSLGIAISEAVE
                     DAATRDDTKYALGSVLNHVLMHQTVIGLEALKQLEMADAWPDVVIGCTGGGSNFGGLV
                     LPFIKHKLEGKAIRIVGAEPAACPSLTKGVYTYDFGDTSKLTPLVKMHTLGHGFVPAG
                     IHAGGLRYHGMSPLISHLLELGLMEAQAVKQTSAFEAAVQFARTEGILPAPEPTHAIR
                     LAVDEALAAKESGEEKTILFGLSGHGHFDLAAYDSYLSGSMQDLEYPAEAVAAAMADL
                     PVVPVG"
     gene            complement(8026..8778)
                     /locus_tag="VHR64_13340"
     CDS             complement(8026..8778)
                     /locus_tag="VHR64_13340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3303863.1"
                     /translation="MNNDYVERNRTAWTGYSSDFAATAEREWAASEPVWGIFLVPESE
                     VGMFPADVSGMDVIELGCGTAYVSAWLAKRGANPRAIDITPAQLETARALQDKFGIHF
                     PLVEGNAEATPFENESADLVISEYGASIWCDPYKWIPEAHRLLRPGGYLRFYRGSFLT
                     QLCSPIDPDAPVGTELIRDQFGLCRIDWGDSVEFTLPHGELIRLMIDTGFEIEGLVEI
                     QVPADAMTRYPYMSAAWASRWPCEEAWKARKR"
     gene            complement(8791..9222)
                     /locus_tag="VHR64_13345"
     CDS             complement(8791..9222)
                     /locus_tag="VHR64_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3303864.1"
                     /translation="MTNPFSAVNHVGVVVSNLDEGARFFTDVLGFVPVPERTGRLAPN
                     GDTLTRRFGVDANADGRFAFFRLGDNLIELLEWSAPGRNETAPLNSDLGGRHLAFSVS
                     DIDAAVERLKSVPGVTIREPNDAGYIYCATPLGLEIQLIPA"
     gene            9362..9889
                     /locus_tag="VHR64_13350"
     CDS             9362..9889
                     /locus_tag="VHR64_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303865.1"
                     /translation="MVATSPDRIRSVNGISDGVREGYRHYQKRISPSLDFEADGIRLK
                     WYDIAFTMRPIGPELDTEAREYLASEIESGRLGINDEIGFVILHDCGDVVFLIVSTWR
                     NSNELWETPYVKLRDNGGGFEPVEVKGIHRPAFCVWEMGAVTHESKAWSRYLSSNRGQ
                     AARETYFFDMYSGLI"
     gene            9896..>10273
                     /locus_tag="VHR64_13355"
     CDS             9896..>10273
                     /locus_tag="VHR64_13355"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX3303866.1"
                     /translation="MYSPTTRLLTILERLQSRRSVSGPELAAELEVDIRSVRRYIASL
                     RDLGIPIDGEPGRAGTYRLRPGYRLPPVMFTNPEMIAVMLGLAAVREMGLAEACGADS
                     AIDKINRVLPDSLRTQAQAIQTAL"
BASE COUNT         1819 a         3343 c         3254 g         1857 t
ORIGIN      
        1 cgctacttac tcgatcgagt tctgcgcacg tttctgctcc agtcctcgcc ggtgtcgctg
       61 ggcactccgc tgcgggtcga tgcgcccacc ctcgagtcgt attgccgtga gcggatgacc
      121 gagctcggcc tggcgccggt ggatgccgag ctaaaggtgg atggggaatc gatcctggtg
      181 acgcaggacg cgggtgggct ggtgatcagc gtcgaccgac tgcaaaagga cctggtgcgg
      241 caactttcgg gattcaccac gccgcgcatc gatctcaccg cgacctattc gccggcgatg
      301 attcgtgcca acgaaatcga gtcggaaatc agctcgcttt catcggtgct gcagcagccc
      361 atgcggctct acaccgatac cgatcaatgg gacatcccgc ccagccagat ggcggccaat
      421 ctcaagatcg accagtcgaa tggccgtccg acgctcatga tcgacgacgc agcgatcacc
      481 ggactggtcg atcggatcgc caacgaaatc gatcaaccgg tcagcaacgg ctcagtggac
      541 gacagcggca tctacgcgcg gatcatcgcc ccgcacgacg gcgtcacggt cgaccgcgcc
      601 acgctcaacc agcgcatcca cgacgcgatc acgactggcc tgaacgaggt tgagatcccg
      661 gtcacgacga caccgtcaaa tggtgacctc aagccgctcc tgaccaaata cggcatcacg
      721 gatctgctcg cgaccggctc gtcgagcttc gtcggctccg atcccggccg caccacaaac
      781 gtccggcgtg cagcccaatt gatcgacggg accctggtgg cgcccggcga agtcttctcc
      841 ttcaaccagg cgctgggctc gatcgttgac gtcggtggat ttgtgccggc cggcgcgacc
      901 gagggcggaa taccggggac ggcggtcgga ggcggcgtct gccaggtctc gacgtcggtc
      961 ttccgtgcgg cactccgggc aggactgccg atcaccgagt ggtatccgca cgcgtaccgg
     1021 agcatgtact acgagcagga tagctgggcg cccggcttcg atgcgtcgat ccagcaaccc
     1081 gatgaggacc cgctgaacgg tccggacctc aagttcacga acgtcaccga cggctggctc
     1141 ctggtccgcg caactgccag cagtaccggc gagctgaagg tcagcctgtt cggaaccgcg
     1201 accggtttcg atatcgtgat ctccgacccg aactatggcg ccatcgtccc ggccgaccag
     1261 gcaccggtcg aagaggtgga cgattcgctg ccgagcggca cgatggatct ctggcagccg
     1321 gcgcgagatg gcgtcacgat gtccttccac cgcacggtct atgcggccga cgggacattg
     1381 ctgatcgatg aggacttcgt ctcgtactat cagccgcaag gaccggtcta ccgggtcagc
     1441 ccggacaagg ccggctcggc gaccgccggc tattgatctt ttccgctcac ggacgtgatg
     1501 ccaaatgccc cgcacactcg tggccctggc cattctttgg tcgatgctcg cgacatcggc
     1561 caacgcaacg ccaacttcac tcccatcccc gccaccgcag ccagcctctg ggccgggcgg
     1621 aagcgactac gcgttcgagc aagtccatgc cgagcacgtt ggcacggcgc ccacgggcta
     1681 ctggctgttc tccccgctgc gcgacgcatc gtccgacccg cttccggtcg tcgtcttcct
     1741 gcatggcttc agcgctgtcg acccggatgg gtatcgcgct tggatcgacc acatcgtgcg
     1801 ccgtggcgca atcctgatct atcccgatta ccagacagat aagctcaccc agacatcttc
     1861 ggctgattac cagcccaacg cgattgccgg gatcatggcg gctgtctcac atctgggttc
     1921 cggtagtgtc gggccggtcg acttgaccgc gatcgcaatc gtcggtcatt cccttggcgg
     1981 ggtcctcgcg ctcaacatcg ccgcggtggc tcgtgagcac gaattaccag cagtcgccgc
     2041 tgtgctgtcc gtcgaaccgg gcggctgcgc cgagtgcggc gggatttcca gattgatcgg
     2101 ccttccattt gccgatctga gcggagtcgg acccgagacg cgggtcatcg tcatggtcgg
     2161 agaggacgac caggtggtca gcgatcacgg agcgaggacg gcgtggaact tgctgaccaa
     2221 tgtgccagcg gaccatcggg atttcgttcg aatgcacagt gatcaccggg gtagtcacgc
     2281 gctgatcgcc gatcattacg cgccactggc tgcctcaagt ggttgggacg tcgacgcgat
     2341 ggactggttt gggacgtgga agctcttcga tctgctgacc ggctgcgcat tccacgatct
     2401 cggctgctcc acctcattcg gaggatcaga gatcgagacg aacatgggcg agtggagcga
     2461 cggcacgacg gtcatcccgc tctcagtgac ggacacgccc tgatgcgcac gatctcgatc
     2521 gaggcaaccg ccggctattc gctggcccaa accgcggcgc ccgtcgcgtg gggaggcggt
     2581 cgctggccga atcaggactg gatcggagga cgcctgatca cggtatcggt cgagcagagc
     2641 tcgtcctgtc tgcgcatcgt gcaccagccc gggccggagt cgacggtgct ggagctggag
     2701 accgatcgtc ccgagctcga tcaccaggac tgggcagatc gcgttcttgg cgtccgacgc
     2761 attgcgccgg cgattgggga tcccgtggtc cgctcgattg ccgagcggtt tcccggtatg
     2821 cggccgtaca gcaatggcag cttgttcgca ggcatcgtga ccgcgattgt cgggcagagc
     2881 atctctgtgc aagcggccgc ggtcaccgag cgcaagctcg cagccctttt cgccgctcca
     2941 atcaccatcg gcgagcggca ttactggccc gagccgagcg tccggcagct cgccgatgct
     3001 tcgccgcacc ttgtccggca atcaggcgtc acacagcgca gggctgatgc aatcgtgcac
     3061 attgcccggc tggccacaga gaaccagctc cttgcgcctg gcgtagatcc ggatgatgag
     3121 caggcggtat tcgcggcaat gctgcgcctg ccgcaggttg gccggtggac ggccgaatcg
     3181 gtcctgctct ggggcctggg atcggacgat gcctaccctg ctggcgacgt ggcgctctta
     3241 cgcgcggccc ggctggccta cgctcgtccg gagatgacgc acggggagat ggatactctc
     3301 tcggaatgct ggacaccggg acgatcatgg gcagcacgct ggctctggct gaacctgttt
     3361 gggccagcgc cccagcctag tccaccgggt tgagccccat ctatactaag ttcgccattc
     3421 ggaccgagcg gccgatattt ctgttaattc cccctcgcga ggaaccaatc tgaatgccac
     3481 attttgatct ggagggcttc atcaaggcgg tctcgtactt cggcgtggcc ggagtggtct
     3541 ttgccgagac cggcttgttc ttcggcatct tcctgccggg cgatagcctc ctctttactg
     3601 ccgggttcct ggcctcgcag ggacacctga acatattcct gctctgcctt gtctgcttcc
     3661 tggccgcagt agtgggcgat tccgtgggct actacatcgg cgatcgcatg ggccgccgac
     3721 ttttcaccaa gccccaatcg cgcgtgttca agccgtcgaa cctgatcaag gcacaggcat
     3781 tcttcgacaa gcacggcggc aaggcgatca tcctcggtcg gttcatgccg atcgtgcgga
     3841 ccatggtgcc gatggtcgcc ggcgcaggga caatgcagta ccggcgcttc ttcacctaca
     3901 acgtcgtcgg cgcgtttctc tggggtgtct gcattccgat ggccggttac ttcctcggca
     3961 gcacgatccc gggcgtcgac aagtacctgc tgccgatcat cctggtcatc atcgtcgtct
     4021 cgatcgctcc gtccgcgtac cacatctgga aggagaacgg cgagctgatc aaggcagagg
     4081 ctcgccggcg gttgaccgaa cgtcgaacct agaataaccc ggccagcgca gtgagcatgc
     4141 cggatgtgcc ggtcaggatc agcagcgcaa gcgtgatccg gcgaaactgc gcctgatcaa
     4201 cccggtcgtg caggcgctgg ccaatccgcc ggccgataag cgcgaccggc aacagtgcag
     4261 ccgcaatccc gaactcgcgg ccgccgaccg cgccggtccg gacgaccagg aaaatcccga
     4321 ccagattcat ggccgagaag tagaacgtga tgctggcgcg gaagactttc ggctccggcg
     4381 cgcgatcagt caggaacagc acaactggcg gtccggacat gccggtcgat gttccgagga
     4441 tgccactcgt caaccccgcc acgaccggag caatgcgcga gcggatgccg gggatcacga
     4501 acccgcgcgc gaccatgatc gcgaacacga ccacgatgaa accggctgcg agcttgatgt
     4561 agcgggtgtc gaccccggtc aggatccgga cgccgatgag cagtccgagc aaggccggca
     4621 ccatgagcgg ggagacgatc ctgacccgta tcttgtcctt gtcctcgatc aggatcggaa
     4681 gcccggaggc aatcgcgagg ctcgaggtca ggaccacgac cgctggcggg gggaaaagca
     4741 tcagcatgaa cggcacgatc acgagcccga aaccgaatcc cgtcagcccc gagatcgtcg
     4801 cgccaagcgt caccgcgcac agtgccgcaa ttgcctgcca ccacgccaaa tccagcgcat
     4861 tccaggcact ctgcatcaaa tccggcaccg gcgatcaaaa ctccacgcta gatttctcga
     4921 ctcgtgaatg atcgcattcg gatcgatccg aatgaggatg tgctatgccg gcggtgcgga
     4981 ccagaggacc tcgccgccga ggatcgtcct ccagatcggg atgtcgcgga tttcggcggg
     5041 tgcggcggcg ttgaggtcgc cgtcgaggat cgcaatgtcg gccagtttgc cgacctcgag
     5101 cgatccgagc cgatcctcca tccggatcga gaccgccgcg tcgatcgtgt gcgcccgcag
     5161 tgcctcttcg agcgtcagcc gctgctcctc gccgatcggc tggccctgcc gcgtcttgcg
     5221 atctagggcg ttcgcgatcg tgtggagcgg gttgaacgag gtcacgaagg catcgctcga
     5281 gaggaccggc ttgatccccg catccagctc ctcccgcatc ggctcgagcc gatgcgcgcg
     5341 ttgacccagc cgggacacga actcgtcgcc gcaatcgcag agcatgttcg gctggttaac
     5401 cgggatgatc ccaaggctcg ccatccgctc gatctgctgc ggcgtcgggt agaagcagtg
     5461 ctcgatccgg tggcgcgtgt cgagccgagg gttggcccgc agcccggcct cgatcgcgtt
     5521 gatggtcatc tccatcgccg cgtcgccgcc ggtatgaatg ccgacctgcc agccggccgc
     5581 atgcgcccgg gtgaccagct cagccagctc gttcggcttc cagtaggtcg agccggtgaa
     5641 ctggccctgc tcgccatacg gctcggtgaa ccaggccgtg ccgccgatca atgtaccgtc
     5701 agagtagagc ttcatcggac caaccatgag ttgatcgtcg ccgaatggac cggccatgcc
     5761 gatgctgatg aagtcgtcga ggtggttcga gagcggcatc atgattaccc tgagccgaag
     5821 tacaccctgc cgatgcgcct ccctgtaccc cttcatctcc cggcgggtca cttgcgggtc
     5881 gcagatggtg gtcaatccga ccttcaggta ctcgtgactg gcggcatcga gcagcgcgac
     5941 cgtgtcctgg cccggcagaa tggtgtggaa gttcgggcca tggcagccaa tgtcgagctc
     6001 gacgggcagg atcttgctcg tcgccgagtc aaggcagagc ccggtcaggc ggccaccctc
     6061 atcccgcacg aacttgccgc cgctcggatc ggccgcgctc tccaggatgc ctcgatccag
     6121 cattgcggcg gtgttgacga gcgcatgatg cccggagacg tggtagacga tgaccgggtg
     6181 gacctgcgtt gccgcgtcga gatcgtcgcg cgtcggcaat cgtccgtccg ggaacttggc
     6241 ccaatcgagg ctgaatccgc ggatccagcg cccatccggc gtgttctccg ccgcctcggc
     6301 gattgcgcgg gtcaggtcgc tgatcgaggc gaccgccggg tagcggagat cgaccgaggc
     6361 gagctgctca gcggtcgcca ggtagtggtt gtgaacgtcg aggaagcccg gcagcaccgt
     6421 gcgtccgcct gcgtcgatct cctccgcatc gcgaccgaga cgcgcgcggg cgtccgattc
     6481 cgtaccaacc gcaacgatcc ggccgtcccg gaccgcaact gcctcagcag tgggttgcga
     6541 ctcgtttccg gtccagaccg agccgttccg gatcagcatc tccattgcgc tgccgatcag
     6601 ccgactggca cgaccggcag gtcggccatc gccgcggcga ccgcctctgc cgggtactcc
     6661 agatcctgca tcgagccgct caggtagctg tcataggccg cgaggtcgaa gtgaccgtgt
     6721 ccgcttaacc caaagagaat cgtcttctcc tcgccggact ccttcgccgc cagcgcctcg
     6781 tcgaccgcca gccgaatcgc gtgcgtcggc tcgggcgcgg ggagaatgcc ctcggtccgc
     6841 gcgaactgga ctgccgcttc gaacgccgag gtctgcttga ccgcctgcgc ctccatcaag
     6901 cccaactcga gcagatggct gatcagcggc gacatgccgt gatagcgcag accgccggcg
     6961 tggatcccgg ccggcacgaa gccatgcccg agggtgtgca tcttgaccag cggggtcagc
     7021 ttcgacgtat cgccgaagtc gtatgtgtag acgcccttgg tcagcgacgg gcacgccgcc
     7081 ggctcagcgc cgacgatccg gatggccttg ccttccagtt tgtgcttgat gaacggcagg
     7141 acaagaccac cgaagttcga gccgccgccg gtgcagccga tcaccacatc aggccaggca
     7201 tcagccatct cgagctgctt cagcgcctcg aggccgatca ccgtctgatg catcaagaca
     7261 tggttgagga cggaaccgag cgcgtacttg gtgtcatccc gcgtcgcggc atcctcgacg
     7321 gcctcggaga tcgcgatgcc gagtgacccg gtcgactccg gatcctgggc caggatcgcg
     7381 cggccggcat tcgtgtccgg gctgggggag gggacacagg tcgcgcccca agtctccatc
     7441 agagcccggc gatacggctt ctgcgcgtac gagatcttga ccatgtagac cttgagctcg
     7501 aggtcgaaca tctggcaggc caatgccagc gagctgcccc attgcccggc gccggtctcg
     7561 gtcgcgatcc gcttgacgcc ctcctgcttg ttgtagaagg cctgcggcac tgccgtgttc
     7621 ggcttgtggc tgccggacgg gctgacgccc tcgtacttgt agaagatgcg gacccgctcc
     7681 ggcagcccga gctccttctc gagtcgatgg gcgcgatgga gcggagtcgg ccgccagagc
     7741 cgataggcgt cccgaacctc atccgggatc gggatccagc gatcctgcga gacctcctgc
     7801 ttgatcacct ccatcgggaa gatgggggcg aggtcggccg gcccgatcgg ctggagtgtg
     7861 ccggggtgca acgggggcgg gacgggcgat ggcaggtccg ccgcgatgtt gtaccagtcc
     7921 ctggccaggt cgtcttcagt cagcaggaat ttggtcgtgt cggacatgat gctctccggg
     7981 gctgcgcccg cttgaacgtc gtttgtcaat taccgctgga ttgtactagc gcttgcgggc
     8041 cttccaggcc tcttcgcacg gccagcggct ggcccatgcg gcactcatgt acgggtagcg
     8101 ggtcatcgcg tcggccggga cctgaatctc gaccagtccc tcaatttcga agccggtgtc
     8161 gatcatcagc cggatcagct ccccatgcgg gagcgtaaac tcgaccgagt cgccccagtc
     8221 gatccggcaa agcccaaact ggtcgcgaat caattcggtg ccgaccggcg catcgggatc
     8281 gatcggggag cagagttggg tcaggaagct tccgcgatag aaccgcaggt acccgccggg
     8341 ccgcaggagc cggtgcgcct cagggatcca cttgtacgga tcgcaccaga tgctggcgcc
     8401 gtactcgctg attaccagat cggccgactc gttctcgaaa ggagtcgcct ccgcattgcc
     8461 ctcgaccagc ggaaagtgaa tgccgaactt gtcctggagt gcccgggccg tctcaagctg
     8521 ggccggggta atgtcgatcg ccctcgggtt cgcgcctcgc ttcgccaacc aggccgagac
     8581 gtaggcggtg ccgcagccga gctcgatcac atccatcccg gacacgtccg ctgggaacat
     8641 gccgacttct gactcaggaa cgaggaaaat gccccagact ggttcagatg cggcccattc
     8701 gcgctctgca gtcgcggcga agtcagacga gtacccggtc caggccgttc gatttcgctc
     8761 aacataatcg ttgttcatcg atcgtgccgc ctatgcggga atgagctgaa tctcgagacc
     8821 gagcggcgtc gcgcagtaga tgtacccggc atcattcggc tcccggatgg ttacgcccgg
     8881 caccgacttc aaccgctcga ccgccgcgtc gatatcggaa actgagaacg ccaggtgacg
     8941 accgccgagg tcggaattga gcggcgcggt ctcgttgcga cccggcgcgg accactccag
     9001 cagctcgatc aggttgtccc caagccggaa gaaggcaaac cgaccgtcgg cgttcgcatc
     9061 gacgccgaat cgccgggtca gggtgtcgcc attcggcgcc aaccgaccgg tccgctccgg
     9121 caccggcacg aatccgagca cgtcggtgaa gaaccgggcg ccttcgtcca ggttagatac
     9181 gaccacaccc acgtgattga cggctgagaa tgggtttgtc acgcgcgttt tatcccttgc
     9241 atcatcgacc agctaactcc cgccgatcat acgcgccagt ggccggatac aatgcggacg
     9301 aggcctgtcc gcattgtccg ctattctgcg acccgattga agagcaggag aggagcagct
     9361 tatggtcgca accagtcccg atcgaatccg gtcagtgaac ggaatatcgg acggcgtccg
     9421 cgaaggctat cgccattacc agaagcggat ttcgccgtcg ctcgacttcg aggcggatgg
     9481 cattcgtctc aagtggtacg acattgcctt cacgatgcgg ccgatcggtc cggagctcga
     9541 taccgaggcg cgggagtacc tggcgtcgga aatcgaatcg ggccggctcg ggatcaatga
     9601 cgaaatcggg ttcgtgatct tgcacgattg cggcgatgtg gtcttcctga tcgtctcgac
     9661 ctggcgcaac tcgaacgagc tctgggagac cccctacgtt aaattgcgcg ataacggcgg
     9721 cggcttcgag ccggtcgagg tcaaggggat ccaccggccg gcattctgcg tctgggagat
     9781 gggggcggtg acacacgagt cgaaggcgtg gtcgcgctac ctgtcgtcaa accggggcca
     9841 ggccgcccgc gagacttact tcttcgacat gtattcaggc ctgatctgac ccgaggtgta
     9901 cagtccgacg acacgcctgc ttaccatcct cgagcggttg caatcccgcc gctcggtctc
     9961 cggccctgag ctggcggccg agctcgaagt cgatatccgc agcgtgcgtc ggtacattgc
    10021 ctcactgcgg gacctcggta tcccgatcga cggcgagccg ggtcgcgccg ggacctatcg
    10081 tctccggccg ggatatcgcc tcccgccggt gatgttcacg aatcccgaga tgatcgcggt
    10141 catgctcggc ttggcggcgg tgcgcgagat ggggctggcc gaggcctgcg gagccgacag
    10201 cgccatcgac aagatcaatc gcgtcctgcc ggattcgctg cgcacccagg cccaggcgat
    10261 tcagaccgcg ctc
//
LOCUS       DATAOZ010000525         9677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3104551, whole genome shotgun sequence.
ACCESSION   DATAOZ010000525 DATAOZ010000000
VERSION     DATAOZ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9677
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3104551"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VHR64_13360"
     CDS             <1..966
                     /locus_tag="VHR64_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3303867.1"
                     /translation="NPNHYDTVPNIDEFVMVVQPETDTLVRSLESGDIDLVEILPAPD
                     TATIQGTAGLKVAIYDIYSITYFVMNLDGQRVPAFSDQRVRQAMYQAIDRQSITDNIF
                     LGYGEAAIGTQPPLSPSYDPAKMQPEYTYDPNAAKQLLTDAGWTDTNDNGVVDKDGKD
                     LKLSIAYVGGDATVDSMLSYLQEAWKAIGVEVQLENISGEVLQQQLLDGNFDLSLLAI
                     NLTPDGSQGLLFTCEAQTTGFNFGKYCNQEYDALDAQQLREFDPTMREQMQVQLSRIV
                     WRDLPIGPIRFGVARTGYSAHIHNFFPNGYGFLWSLPFVWVDSGS"
     gene            1065..2411
                     /gene="rseP"
                     /locus_tag="VHR64_13365"
     CDS             1065..2411
                     /gene="rseP"
                     /locus_tag="VHR64_13365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX3303868.1"
                     /translation="MFSDAFANGLWIIPVLAVLILVHELGHFFAARACGVKVEEFGIG
                     IPPRVIGWTRNGVIWSINAIPFGGFVRVKGEDGANMDPDSMNAKPPLQRAFFLAAGAG
                     MNILFAIVLMFVVLGVKGDLHQNVYIDEVSGGSPAAKAGWQSGDRVAKVNGESVESTT
                     EVANLTAEFGDRQMEVTIERRGQLINTYVTPRKNPPEGQGRVGILMQQENVSIVQVDA
                     VQPGSAAEAAGIQAGDEFVSVNGRPIEDFFSLKHELSLFGGAGVDVVVRRGDALYSTT
                     VAVPNLGPRQDVFAALGLPTLRSIPQFEQIPLTEIVPRGFSESFHATKLMIQQIKTLF
                     TDREALKQVAGPVGMGQITSELIRESPLPVWFILAQLSIILSLNLAVLNLLPLPALDG
                     GRLFFVLIELIRGGRKIAPQKEGVVHFVGFVLLIGLMFLIAFRDVDRILGGHSFLP"
     gene            2444..3619
                     /gene="ispG"
                     /locus_tag="VHR64_13370"
                     /gene_synonym="gcpE"
     CDS             2444..3619
                     /gene="ispG"
                     /locus_tag="VHR64_13370"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX3303869.1"
                     /translation="MLVAPRRKTRILEVGTVKIGGENPIVVQSMTTADTRDPKATLEQ
                     IAKLANAGCEVVRVAVPDRVAAAALPDIVPYSPIPVIADIHFEHTLALSAMKAGIHGL
                     RLNPGNIRKPEAVREVVAMAKERGTPIRIGVNYGSVPPFTDEFVDEMNAQNATQAELR
                     AEWMVRTALTHIRILEDHDFGLIKVSLKAFEVPVLFEAYRRFAALPNDYPLHLGVTEA
                     GTPKAGSVRSAVGLGTLLALGIGDTIRVSLTTDPVEEVFVGYEILKTLELRQKGATMI
                     ACPTCGRAEVDLFSLAEKVDEFLKTIDEPIRVAVMGCEVNGPGEARDADIGLAAGRKM
                     GVIFRKGKILKRVTEAEMLDELKAEILRAAEDKRNGVEVESHHNPNVYKPTLTLTSV"
     gene            3653..4348
                     /locus_tag="VHR64_13375"
     CDS             3653..4348
                     /locus_tag="VHR64_13375"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX3303870.1"
                     /translation="MTTFTVPDFKLDAAMVERLKQYDSPTVANAIETFKVRDRTEGYI
                     GGYVRALYDAGVMVGQALTVTIGQKPGKVAGRDGYWAMWEALERMSSPAVIVMQDVTG
                     TPERYACAGEVMATLAKRLGAVGMVADGGLRDIAEVQALGFHYYAAFPVVSHGNFEIL
                     DVGVPVEIDGQVVHTGDILHGDINGIVIIPNDVLDGLEDAIKVIRDREARFMNFIKGE
                     NFTLEAARAGTGY"
     gene            4351..5103
                     /locus_tag="VHR64_13380"
     CDS             4351..5103
                     /locus_tag="VHR64_13380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX3303871.1"
                     /translation="MEPALYIDALEREGTRLADTADAIDPNAAVPTCPGWTLRDLLQH
                     LGGVHHWAGEHIRGRLPQVLRVDDFRDLVGEWPDDTALVDWYRGELDLLLDTFRAAPR
                     DIDCARFLKSPDPLSFWTRRQAHEATIHRADVEAIIDRLTPVDTPFALDGIDELVDGF
                     VPRPFMKLRSEQPLTLGIAPSDSDLVWLLTISEGPVTISTERAESDCTVGGSASDVYF
                     ALWNRADDARLDISGRRDVFDLFRTKIAIKWS"
     gene            5222..6331
                     /locus_tag="VHR64_13385"
     CDS             5222..6331
                     /locus_tag="VHR64_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3303872.1"
                     /translation="MRAIRFVFVVALFAMLAPLSFSGEQVRAAQPLTSCEPVKHAAAT
                     PTASSTPLKVSATPTSGLKKVSIGYIPVMNFAPMFVAKDLGYFAEQGLDVELRTFAGG
                     SEMIASLATNDLNVGMGGAGPGLFNAVNQGLPVKIIAPGHSEGNPVATPLVVAKAKCE
                     SGEITQVSDLKGQKVAINAPGATEYWLSQALATGGLTMDDVDLQTLPFSDAVVALQSG
                     AIDAAILGEPTATLAEQQGIGVRLVTDFDVQGIQPTMVYASEEFLNNDPEAAKGVAIG
                     YMKAVRAIMERGFSDPEILPIIASYTNLPEDLITESVAPLFTVDGSINVDGLNTLQSF
                     FRSMGELNYDTDIDPNTMIDRTALDAALQELDSSQ"
     gene            6346..7212
                     /locus_tag="VHR64_13390"
     CDS             6346..7212
                     /locus_tag="VHR64_13390"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3303873.1"
                     /translation="MAIAASRRTPHVTHPVGKTAQPTTYRPGGTLIRVDSLTRTFESP
                     SGTLNALEDISLDIRTGEFLAIVGPSGCGKTTLLRILAGLDKPTTGKLEAPGRFPTNA
                     LVFQGRSVFPWMTVEDNITYGLKLTGVPRSERRERADSLLRLIGIERFRKSYPHQLSE
                     GMRQRVAIARALAVDPDLLLMDEPFSALDEQTKLILQDEVLDIWERTRKTIVFITHSI
                     DEALVMADRIAVFTAQPGRIKAIIDVDFPRPRDFLAVRSDPHFAERFRCVWELLRDEV
                     TIASQHPAERPR"
     gene            7209..7985
                     /locus_tag="VHR64_13395"
     CDS             7209..7985
                     /locus_tag="VHR64_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3303874.1"
                     /translation="MSRRRWELLLSLLGPLVVLGIWEFLSRTRIINPLFWPAPTSLID
                     TLQQEVSSGALFTNVRISMVRVLIGFLIAAVPGVIIGLAMGLYWPVRVFLMPVATAIY
                     AIPKLAVLPLMLIVFGVGESSKIAIIVVSIFFLIVLNTMSGVMGIDPTYRDVAQNLGA
                     SRWQLFTTVAWPGALPAIFTGFRLAMGFALLVIVGTEFLDGNKREPNGIGWYIIRSWN
                     LLKVDDLFVGLLVVALIAWILNVLLEAIERRLLPWRAEVM"
     gene            7987..8919
                     /gene="menA"
                     /locus_tag="VHR64_13400"
     CDS             7987..8919
                     /gene="menA"
                     /locus_tag="VHR64_13400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HEX3303875.1"
                     /translation="MSALAPAAAAPSPIRIWMRAVRIFSFTASVTPVLIGTAFALVSR
                     DFSIVRFVLVALACVLCHAGCNLANDYFDHRKGIDNRESIGIGGVIQDGWLTAEQVRN
                     GMLVCFATATILGIAILALSTWWLLALAVPSLLAAILYTGGPKPLGYIALGELTVWLF
                     MGMGIVCGTYVAMAEHLSWEVAVGSLGISALVAAILHVNNMRDFEPDQTAGKRTLAHL
                     LGWSGSVREYEFLIVGAYFFTAILIVIWPSNWPTALVLATAPAALALIQLVRSNADAK
                     SLNMGVRGTAQLHFRFGLLLTAGLVVRTIIERLV"
     gene            8951..>9677
                     /locus_tag="VHR64_13405"
     CDS             8951..>9677
                     /locus_tag="VHR64_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3303876.1"
                     /translation="MSRRALLQGGLTGVLAAAGLTAFEIYQARYVNPYSPQLERLDLA
                     VPASSEGLAGMKIGFITDTHVGPFVETGDLYRALDLIRAESPDLILLGGDYISESPRY
                     APETAAAIADLLSDAPLGGYGVIGNHDLSVSRTRVQEALVTVGVPVLRNQTTVVTYGG
                     AHLAIAGIDETLLGDPRPVETIAQAPAGVPVLALWHEPEFAEQAAAAGAFAQLSGHTH
                     GGQIRLPGIGPVGLPVHGKRHVIG"
BASE COUNT         1697 a         3057 c         3052 g         1871 t
ORIGIN      
        1 aatccgaacc attacgacac ggtgccgaac atcgacgagt tcgttatggt cgtccagccg
       61 gagactgaca ccctggtccg gtcgctcgaa tcgggcgaca tcgacttggt cgagatcctg
      121 ccggccccgg atacggccac gatccagggc actgccgggc tcaaggtcgc tatctacgac
      181 atctactcga tcacctactt cgtcatgaat ctggatggcc agcgtgtccc ggccttctcg
      241 gatcagcgtg tccgccaggc gatgtaccaa gcgatcgacc gccagtcgat caccgacaac
      301 atcttcctcg ggtacggcga ggcagccatc ggcacccagc cgcccttgtc accgtcctat
      361 gacccggcga agatgcagcc ggagtacacc tacgatccga atgcggcgaa gcaactgctg
      421 accgacgcgg gctggacaga taccaacgac aacggcgtcg tcgacaagga cggcaaggat
      481 ctgaagctgt cgatcgccta cgtcggcggg gatgcgacag tcgattcgat gctctcatac
      541 cttcaggagg cgtggaaagc gatcggcgtc gaggtccagc tcgagaacat cagcggtgag
      601 gtgctccagc agcagcttct ggacgggaat ttcgacctgt cattgctggc gatcaacctg
      661 actccggatg gcagccaggg gctgctattc acgtgtgagg cgcaaacgac cggtttcaac
      721 ttcggcaagt actgcaacca ggaatacgat gcgctcgatg cccagcagtt gcgcgagttc
      781 gatccgacga tgcgcgagca aatgcaggtc cagctctcgc ggatcgtctg gcgtgacctg
      841 ccgattggtc cgatccgctt cggcgtggcg cggactgggt acagcgcgca catccataac
      901 ttcttcccga acgggtacgg attcctttgg agcctgccat ttgtctgggt cgattcggga
      961 tcctgatcgg cgattttggc ggtcgttatg gccgccgggt gctaccataa atacagcacg
     1021 cgaccactcg tgtcgtatcg gcgtggtcgc aaaggagctc cttattgttc tctgacgcct
     1081 tcgcgaatgg gctctggata atccccgtcc tcgcggtcct gattctcgtt catgaactcg
     1141 gccacttctt cgccgcgcgg gcgtgcggag tcaaggtcga ggagtttggc atcggcatcc
     1201 ctccccgagt catcggctgg acccgtaatg gcgtgatttg gtcgatcaac gcgatcccgt
     1261 tcggcggatt cgttcgcgtc aagggcgaag acggcgcgaa tatggatcct gattccatga
     1321 acgccaagcc gcccctgcag cgggccttct tcctggcggc cggtgccggt atgaacatcc
     1381 tcttcgctat cgtgctgatg ttcgtcgtgc ttggcgtcaa gggcgacctg catcaaaacg
     1441 tctacattga cgaggtctcc ggcggctcac cagccgcgaa ggcggggtgg cagtcgggcg
     1501 accgggtggc gaaagtcaac ggcgaatcgg tcgaatccac gaccgaggtc gcgaacctga
     1561 ccgccgaatt cggcgaccgc cagatggaag tgaccatcga gcggcgcggt cagctcatca
     1621 acacttacgt gactccgcgc aagaatccgc cagaaggcca gggtcgcgtc ggcatcctga
     1681 tgcagcagga aaacgtcagc atcgttcagg tcgacgctgt ccagcccggc tccgcagcgg
     1741 aagcagccgg gattcaggcc ggtgacgagt tcgtatcggt caacggccgg ccgatcgagg
     1801 acttcttctc gctcaagcac gagctgagcc ttttcggcgg cgccggcgtc gatgtcgtcg
     1861 tgcgaagagg tgacgcgctg tactcgacga ccgtcgcagt gccgaatctc ggaccgcgcc
     1921 aggacgtctt tgccgcgctc gggttgccga ctctgcgctc aatcccgcag ttcgagcaga
     1981 tccccttgac cgagattgtg ccgcgcggtt tctcagagtc gttccacgca accaagctca
     2041 tgatccagca gatcaagacc ctatttaccg accgcgaggc gctcaagcag gtggcgggac
     2101 cggtcggcat gggtcagatc acgagcgagt tgatccgcga gagtccgctt ccggtctggt
     2161 tcatcctcgc gcagctgagc atcatcctgt cactcaacct ggccgtgctg aatctcttgc
     2221 cgctgccggc gctcgatggc ggccgtctct tcttcgtgct gatcgaattg atccggggtg
     2281 gccgcaagat tgctccgcag aaggaaggcg tcgtccactt tgtgggcttc gtcctgctga
     2341 tcggcctgat gttccttatc gccttccggg atgtcgatcg catcctggga ggtcattcgt
     2401 tcttgccgta gcgcaccagc gcgatcgcgg agatatcaca cacatgctcg tcgcacctcg
     2461 tcgcaagacc cggatcctgg aggtcgggac ggtcaagatc ggtggtgaga acccgatcgt
     2521 cgtccagtcg atgaccaccg ccgacacgcg ggatccgaag gcaaccctgg agcagatcgc
     2581 caagctggcg aacgccggtt gcgaagtcgt ccgcgtggca gtcccggatc gggtcgccgc
     2641 cgcggcactg ccggacatcg tcccctactc gccgatcccc gtcattgccg atatccactt
     2701 cgaacacacc ctcgcgctct cagcgatgaa ggcaggcatc cacggattgc gcctcaaccc
     2761 gggcaacatc cgcaagccgg aggcggtccg cgaggtcgtc gccatggcca aagagcgtgg
     2821 gacgccgatc cggatcggcg tcaattacgg ctccgtgccg ccgttcaccg atgagttcgt
     2881 cgatgagatg aacgcgcaga acgcgaccca ggccgagctg cgggccgagt ggatggtgcg
     2941 gaccgcgttg acccacattc gcatcctcga ggatcacgac ttcggcctga tcaaggtcag
     3001 cctgaaggcg ttcgaggtcc cggtcctgtt cgaggcgtat cgccgatttg ccgcactgcc
     3061 gaatgactac ccgctacacc tcggcgtgac cgaggccggc acgcccaagg caggctcagt
     3121 ccgctcggcg gtcgggctcg gcacattgct cgcacttgga atcggcgaca cgatccgtgt
     3181 ctccctgacg acggacccgg tcgaagaggt cttcgtcggg tacgagatcc tcaagacgct
     3241 cgagctgcgg cagaagggtg cgacgatgat cgcctgccca acatgtggcc gagcggaggt
     3301 cgatctcttc tcgctggccg agaaggtcga cgagttcctg aaaacgatcg acgagccgat
     3361 ccgggtggcg gtcatgggct gcgaggtgaa cggtcccggt gaggcgcgcg acgccgacat
     3421 cgggctggct gccggtcgca agatgggagt catcttccgc aagggcaaaa ttctcaagcg
     3481 cgtgaccgag gccgagatgc tcgacgagct caaggccgag atcctgcgcg ctgccgagga
     3541 caagcgcaac ggcgtcgagg tcgagtcgca ccataatccc aatgtctata agccaaccct
     3601 gactctgaca tcggtctagt cggttcgatc aatcgcgaat ggagcgtgct tcgtgaccac
     3661 gtttaccgtg cctgatttca agctcgatgc cgcgatggtg gagcggctga agcagtacga
     3721 cagcccgacc gtcgccaacg cgatcgagac cttcaaggtc cgcgatcgga ccgagggcta
     3781 tatcggcggg tacgtgcggg cgctctatga cgcgggggtg atggtcggac aggcgttgac
     3841 ggtcacgatc ggccagaaac cgggcaaagt cgctggacgc gacgggtact gggcgatgtg
     3901 ggaagcgctc gagcggatgt cgtcgccggc cgtaatcgtc atgcaggacg tcacgggcac
     3961 gccggagcgg tacgcttgcg ccggtgaagt gatggcgacg ctcgccaagc gactcggtgc
     4021 ggtcggtatg gtcgctgacg gcggcctgcg ggacatcgcc gaagtccagg cgcttggatt
     4081 tcactactac gccgcattcc cggtcgtcag ccacggcaac ttcgagatcc tcgatgtcgg
     4141 cgtgccggtc gagatcgacg gccaggtcgt ccacaccggc gacattctcc acggcgacat
     4201 caatggaatc gtgatcatcc cgaatgacgt gctcgacggt ctcgaggacg cgatcaaggt
     4261 catccgcgat cgcgaggccc gcttcatgaa tttcattaag ggcgaaaact tcaccctcga
     4321 ggctgccagg gcaggcaccg ggtactgacc atggagccag cactgtacat cgacgcgctc
     4381 gaacgcgaag gcacacggtt ggccgatact gccgatgcga tcgatcccaa cgcggcggtt
     4441 ccgacatgcc cgggttggac tctccgcgat ctgctccagc atctgggcgg cgtccatcac
     4501 tgggccggcg aacacatccg cgggcgcctg ccgcaggtcc tgcgagtcga cgacttccgg
     4561 gatctggtcg gtgaatggcc ggatgataca gcccttgtcg actggtaccg cggcgagctt
     4621 gatttgctcc tcgatacgtt tcgggcggca ccgcgcgata tcgactgcgc ccggttcctg
     4681 aagtcgcccg atccgctctc attctggacg agacgacagg cacatgaggc aacgatccac
     4741 cgggcggatg tcgaggcgat catcgaccgg ttgacgccgg tcgacacgcc gttcgcgctc
     4801 gacggcatcg atgagctggt cgacggcttc gtgccgcgcc cgttcatgaa gttgcgctcg
     4861 gagcagccgc tcacgcttgg catagcgccc agcgacagtg atcttgtctg gttgctgacg
     4921 atcagcgaag gcccggtcac gatctcgacc gagcgcgccg aatccgattg cacggtcggc
     4981 ggcagtgcaa gcgacgtcta ctttgcgctc tggaaccggg ctgacgacgc gcggctggat
     5041 atatccgggc gacgcgacgt attcgacctg tttcggacaa agatcgcgat caaatggtca
     5101 tgaaagtgac tattgctaca ctctgccggc gccggactgg tcgttggtag acttgcctgc
     5161 gcgggatgtt cggacggggg tcgtcttgaa atcgaccgtt tccggtacac gggagttgac
     5221 aatgcgagcg attcgatttg tcttcgtcgt ggcgctgttc gccatgctgg cgccgctcag
     5281 cttcagtggt gagcaggtcc gggccgcaca gccgcttacc tcctgcgaac ccgtgaagca
     5341 cgccgcggcc acccccaccg caagctcgac accgctcaag gtctcggcga ctccgacgag
     5401 tggtctcaag aaagtctcga tcggctacat cccagtcatg aacttcgcgc cgatgttcgt
     5461 cgccaaggat ctcggctact tcgccgagca gggattggat gtggagctgc gcaccttcgc
     5521 gggcggctcc gaaatgatcg cctcgctcgc gaccaacgac ctcaacgtcg gtatgggcgg
     5581 cgccggaccg ggcctgttca atgccgtgaa ccagggcctg ccggtcaaga tcatcgcgcc
     5641 gggtcactcc gaagggaatc cggtcgccac gccgctggtc gtcgccaagg cgaagtgcga
     5701 gtccggcgag atcacgcagg tctcggacct caagggccag aaggtcgcca ttaatgcacc
     5761 cggcgcgacc gagtattggc tgagccaggc gctcgcgacc ggcggtctga cgatggacga
     5821 cgtagacctc cagacgctgc ccttctctga cgcggtggtc gctctgcagt cgggcgcgat
     5881 cgatgccgcg atcctgggtg agcccacggc gacgctggcc gagcaacaag gcatcggcgt
     5941 tcgcctcgtg accgatttcg atgttcaggg cattcagccg accatggtct acgccagcga
     6001 ggagttcctc aacaacgacc ctgaggccgc caagggcgtg gcgatcggtt acatgaaggc
     6061 ggttcgcgcg attatggagc gcggcttcag cgatccagag atcttgccga tcatcgcgtc
     6121 gtataccaac ctgccggagg accttatcac cgaatcggtc gcgccgctct tcactgttga
     6181 cggctcgatc aatgtcgacg gcttgaatac cctgcagtcg ttcttccgct cgatgggcga
     6241 gctgaactat gacactgaca tcgatccaaa cactatgatc gatcggacgg cgctcgatgc
     6301 ggccctgcag gagctagatt cgtcgcaata gatgggaacg ccatcatggc aattgccgcg
     6361 tcacggcgaa ccccgcacgt gacgcacccg gtcggcaaga ccgcacaacc aacgacgtac
     6421 cgtcccggcg gcacattgat ccgtgtcgat agcctgacgc ggaccttcga atcgccctcg
     6481 ggcaccctga acgcgctcga ggacatatcg cttgatatcc ggaccggcga gttcctcgcg
     6541 atcgtcggtc cgtccggttg cgggaagacg accttgctcc gcatcctcgc cgggctggac
     6601 aagccgacga ccggcaagct ggaggctccc ggtcggtttc cgaccaatgc acttgtcttc
     6661 cagggtcgct ccgtgttccc gtggatgacg gtcgaggaca acatcaccta cgggttgaag
     6721 ctgaccggag tgcctcggtc cgagcggcgc gagcgggccg attccctgct ccggctgatc
     6781 ggcatcgagc gcttccggaa gtcgtacccg caccagctct cggagggaat gcggcagcgc
     6841 gtcgcgatcg cccgggcact ggcggtcgat cccgatctgc tgcttatgga cgagccgttc
     6901 agtgcgctcg atgagcagac caagctcatc ctgcaggacg aagtcctcga tatctgggag
     6961 cggacccgca agacgatcgt cttcatcacc cactcgatcg acgaggcgct cgtcatggca
     7021 gatcggatcg ccgtattcac ggcccagcca ggtcggatca aggcgatcat cgatgtcgac
     7081 ttcccacggc cgcgcgattt cctggccgtc cgctccgacc cgcacttcgc cgagcgcttc
     7141 cggtgcgtct gggagctgct gcgcgacgag gtgaccatcg ccagccagca cccggcagag
     7201 cggccgcgat gagtcgtcgc cggtgggagc tgctcctttc gttgctcggg ccgctggttg
     7261 tgctcgggat ctgggagttc ctgagccgga ccaggatcat caatccgctc ttctggcccg
     7321 cgccaacatc gctgatcgac acgctccagc aggaggtcag ctctggcgcc ctgttcacca
     7381 acgtccggat cagcatggtt cgcgtgctga tcggctttct gatcgccgcc gtgccgggcg
     7441 tgatcatcgg tttggcaatg gggctgtact ggccggtgcg cgtcttcctg atgccggtcg
     7501 cgaccgcgat ctacgccatt ccgaagctgg cggtcctgcc gttgatgctg atcgtgttcg
     7561 gagtcggcga gagcagcaag atcgcgatca tcgtcgtctc gatctttttc ctgatcgtgc
     7621 tcaatacgat gagcggcgtg atgggaatcg acccgaccta ccgcgacgtg gcccagaatc
     7681 tcggcgcgag tcgctggcaa ctgttcacga cagtcgcctg gcctggcgca ttgccggcca
     7741 tctttaccgg gttccgcctg gcgatgggtt tcgccttgct cgtgatcgtc ggaaccgagt
     7801 tcctggacgg caacaagcgt gagccgaacg ggatcggctg gtacatcatt cgttcatgga
     7861 acctgctcaa ggttgacgat ctcttcgtcg ggctgctggt tgtcgcgttg atcgcctgga
     7921 tcctgaacgt gttgctcgag gccatcgagc gccgtttgct gccctggcgc gcggaggtga
     7981 tgtgacgtga gtgcgctggc tccggcggcg gccgcccctt cgccaatcag gatctggatg
     8041 cgggcggtgc ggatcttctc gttcacggcg tcggtgacac cggtcctgat cggcaccgcc
     8101 tttgcgcttg tatcgcgcga cttctcgatt gtccgcttcg tgctggtcgc gctcgcgtgc
     8161 gtcttatgtc acgcaggatg caaccttgcc aatgattact tcgatcaccg aaaaggcatc
     8221 gacaaccggg agtcgatcgg gatcggcggc gttatccagg acggctggct gaccgccgag
     8281 caggtccgca acggtatgct cgtctgcttt gcgacggcga cgattctcgg catcgcgata
     8341 cttgccctgt ctacgtggtg gctgctggcc ctggctgtgc ccagcctgct cgccgcgatc
     8401 ctctacacag gcgggccgaa gccgctcggc tacatcgcgc tcggcgaatt gaccgtctgg
     8461 ctgttcatgg gcatgggcat cgtctgcggc acatatgtgg cgatggccga gcacttgtcg
     8521 tgggaggtcg ccgtcggctc tctcgggatc agcgcgctgg ttgccgcgat cctgcacgtc
     8581 aacaacatgc gcgacttcga gcccgatcaa accgccggaa agcgcacgct tgctcacctc
     8641 ctcggctgga gtggatcggt ccgcgagtac gagttcctga ttgtcggcgc gtatttcttt
     8701 acggcgatcc tgatcgtgat ctggccgtcg aactggccta cggcgctggt cctcgccacg
     8761 gcgccggcgg cactggctct gatccagctc gtgcgtagca acgccgacgc caaatcgctc
     8821 aacatgggcg tgcgtggcac tgcccagctt cacttcagat tcggcctgct cctgaccgct
     8881 ggccttgtgg tgcgtaccat cattgaacga ctggtctgaa tctcatgcag tcgaacgtga
     8941 ggcggcggta atgtcccggc gcgctttgct ccagggaggg ctcaccggtg tgctggcggc
     9001 ggctggcttg accgccttcg aaatctacca ggcccgctac gtcaacccct actctcccca
     9061 gctcgaacgc ctcgatctcg ccgtgccggc cagttccgag ggactggcgg ggatgaagat
     9121 cggcttcatc accgacaccc atgtcggccc gttcgtggag actggtgatc tttaccgcgc
     9181 actcgacctg attcgggccg aatcgcctga cctgattctg ctcggtggcg actacatctc
     9241 ggagtcgccc cggtatgcgc cggagaccgc cgcggcgatt gccgatctcc tctcggacgc
     9301 gccgctcggc gggtacggag tgatcgggaa ccacgacctc tcggtttcgc gtacgcgggt
     9361 ccaggaggcg ctcgttaccg tcggcgttcc agtgctgcgc aaccagacaa ccgtcgtcac
     9421 atacggcggc gcgcacctgg cgattgccgg catcgatgag acgttgctcg gcgatccccg
     9481 gccggtggag acgattgccc aggctccggc gggggttccg gtgctggcgc tctggcacga
     9541 gccggaattc gccgagcagg ccgcggccgc cggcgccttt gcgcagctct ctggccatac
     9601 acacggcggt caaatccggc tgccgggaat cggaccggtc gggttgccgg tccatggcaa
     9661 gcgccatgtg atcggca
//
LOCUS       DATAOZ010000526         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6524622, whole genome shotgun sequence.
ACCESSION   DATAOZ010000526 DATAOZ010000000
VERSION     DATAOZ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6524622"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VHR64_13410"
     CDS             <1..342
                     /locus_tag="VHR64_13410"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX3303877.1"
                     /translation="SIVLESAKASQFEETAAALALLCAAPVELIDRLMQGNRIDALLI
                     PCKAGGLGWPATKAVIRLTPAQGATSESAFEIAKKDFANLSVVAAKRIMRFWQVRASV
                     ALGTMPLPTLS"
     gene            complement(409..684)
                     /locus_tag="VHR64_13415"
     CDS             complement(409..684)
                     /locus_tag="VHR64_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX3303878.1"
                     /translation="MNTRDAILEKLSRAFAPSRLDVVDESHQHAGHAGHREGGETHYR
                     VYIVSDTFRGKSRIERHRMVNETLAAELKGGVHALAIHASAPGENIA"
     gene            808..1437
                     /locus_tag="VHR64_13420"
     CDS             808..1437
                     /locus_tag="VHR64_13420"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX3303879.1"
                     /translation="MSNDSPLFDRIRVKPDRDRRLRAEHPCCEWPGCNGAATHRAPKG
                     RDREKEYWRFCLGHVREYNHSYNFFAGMSNDDIARYQKDAVVGHRPTWKMGMNGGRPA
                     TRSHSARFQPNLDGADDPLDLSRHFGAAGVDGERLRRRETRVIHNAERRALDALGLES
                     GATTAEIKLRFKMLVKRHHPDSNGGDRSTEDRLREIIQAYKYLKSVGFR"
BASE COUNT          268 a          484 c          483 g          284 t
ORIGIN      
        1 tccatcgttc tcgaatctgc caaagcgagc caattcgaag agaccgctgc cgccctggcg
       61 ctcctgtgtg cagcgccggt cgagctcatc gaccggctga tgcaaggcaa tcgcatcgat
      121 gccttgctga tcccgtgtaa ggctggcggg ctcggctggc cggccacgaa ggcggtcatt
      181 cgcctcactc cagcacaagg cgcgacgtcg gagtctgcct tcgagatcgc caagaaggac
      241 ttcgcgaatc tgtcggtggt ggcggcgaag cggatcatgc gattctggca ggtgcgcgcg
      301 agcgtggctc tcggcacgat gccgctcccg acgctgtcct gagccggacg gggacgccgg
      361 cgcatgtcgg atcaattcgg catgcgtacg agtggccgca ttcgcggtct aagcgatgtt
      421 ctcccccggc gccgaggcgt gaatggcaag cgcatgcacg ccgcccttga gctcagccgc
      481 aagcgtttcg ttcaccatgc ggtggcgctc gatccggctc ttcccccgga aggtgtccga
      541 cacaatatag accctgtagt gagtctcgcc gccctcccgg tgaccggcgt gaccggcgtg
      601 ttggtgggac tcgtcgacca cgtcgagcct cgaaggggcg aaggcgcgag acagcttctc
      661 caggatggcg tcgcgggtat tcatgggctg gcggcttagt cgcgagtggg ccctcggtca
      721 atgcggctgt tcaaatcggg ccctgggaga tattcgactt tgtcaagact tgaaggttcc
      781 tggtccacgt ctgcataatg ttcggtcatg tccaacgact cacctctgtt cgaccgcata
      841 agggtcaagc ccgaccggga tcgtcggctg cgcgccgagc acccgtgctg cgaatggccg
      901 ggctgcaacg gggctgcaac ccatcgcgcc cccaagggcc gcgaccgcga gaaggagtat
      961 tggcgcttct gtctcggcca tgtgcgcgag tacaatcatt cgtacaactt cttcgccggg
     1021 atgagcaacg acgacattgc ccgctaccag aaggacgccg tggtcggcca ccgtcccacc
     1081 tggaaaatgg gcatgaacgg cggccgtccg gcgacccggt cccattcggc gaggtttcag
     1141 cctaatctcg atggcgccga tgacccgctc gacctgtctc gtcatttcgg ggcggctggc
     1201 gtcgacggcg aacgcctgcg ccggcgcgaa acccgtgtga tccacaatgc cgaacgccgg
     1261 gcgctcgatg ccctcgggct cgagtcgggc gccaccacgg ccgagatcaa gctgcgcttc
     1321 aagatgctgg tgaagcgcca ccatccggat tccaatggcg gcgaccgctc gaccgaagat
     1381 cgactgcggg agatcattca ggcctacaaa tatctcaagt cggtgggctt ccgctaagag
     1441 gcgattttgt accaggggct agtgtcccga ttcccgaagt tcgcacgatt cccggcaccc
     1501 tcgcttgcga acttcggaa
//
LOCUS       DATAOZ010000527         4152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8629596, whole genome shotgun sequence.
ACCESSION   DATAOZ010000527 DATAOZ010000000
VERSION     DATAOZ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8629596"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1395)
                     /locus_tag="VHR64_13425"
     CDS             complement(187..1395)
                     /locus_tag="VHR64_13425"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX3303880.1"
                     /translation="MIARCPPQLLVSTAVALTLGAPSLGFGQEVGPYEPLGIRAGSFL
                     VFPSLGLTEQYNDNVFADPHNTKDDFITVLEPRVQVQSTFSRHSLGLTAGGNIGFYAD
                     HPDENYQDAFIQGNGRLDVTRDNYFNGGLNFGRAHQGRDDPENNPNQKNTTEIYQYGG
                     SLSYTHLFNRLNVMVTGAVARDDYEPNQDSDRDQNNYIGGLRTGYFVSPRINTYIYGA
                     YAVGKRDQKVDSGGVERDTKTWGAGVGAAVELTNLITSDIRVGYSRQSYAEGSFSDNE
                     GVGYGLDLTWTPTQLTTVRATGAGNFVPTSSEGSDTQSNFQSTAGASVDHELLRNVHL
                     GAHVNYIRDNFNGPSRTDNGVVVGGGVTYFLNRYFSVNAGYDYQQRWSDFPDDEFSRN
                     LVMVGVTGQL"
     gene            1885..2607
                     /locus_tag="VHR64_13430"
     CDS             1885..2607
                     /locus_tag="VHR64_13430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX3303881.1"
                     /translation="MCVYAVGDVHGRADLLEVLHRRIAADADQLTPGTEKIVLYVGDY
                     VDRGLESRQVLDVLLSRPLPDFHAVHLLGNHDAWLLSFLIDSKIGPTWMRFGGDATLH
                     SYGIHLRPSVNDVRYFEDLQAELRERLPRQHVEFLEELELSYETGDYLFVHAGVRPNQ
                     PLDRQIADDLLWIREPFLSSRRDLGKVVVHGHTVEAEPAVRANRIGIDTGACWTGRLT
                     CLVLEEGRYRFLNTAGGIVAQQ"
     gene            2665..3681
                     /gene="galE"
                     /locus_tag="VHR64_13435"
     CDS             2665..3681
                     /gene="galE"
                     /locus_tag="VHR64_13435"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX3303882.1"
                     /translation="MSKPREILVTGGAGYIGSHVTRQLVEAGDRVVVYDNLSSGHAWA
                     VLGAELVVGDLADRERLAASFGGRNIDAVFHFAAHIWVTESIRDPVKYYRNNVAAALG
                     LFEVAAQHGVRRIVFSSTAAVYGQPDCALIDEGMPLAPINPYGASKMMAERILMDIAA
                     ASGLRYAILRYFNVAGADPQGRIGEATPDNQHLIKLACETALGARSAMRIHGTDYPTP
                     DGTCVRDYIHVDDLARAHLAALDYLEQGGDSTVLNCGYGHGASVRLVLDTVRRVTGID
                     FPIEFGPRRPGDPAVLVASNDKIRRVLGWRPRHDDLEFIVRTAWAWEQILQSRRADAM
                     PTAG"
     gene            3772..>4152
                     /locus_tag="VHR64_13440"
     CDS             3772..>4152
                     /locus_tag="VHR64_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303883.1"
                     /translation="MIRIFKPRGLAAVLPALLMLASAALAQAAAPSVGPGSLPGLGAS
                     TPAPELSRERLQGLIDTLEDPGRREQLIATLRTLEAAQAEQPPGEPFLSDDVVAGVLG
                     EITARTDVVRKVSLSILNSLDQIPL"
BASE COUNT          697 a         1338 c         1357 g          760 t
ORIGIN      
        1 caggctggcg atcaggatca tcgcgctcag ccgggtcccc ggcgccgggg ctatcatgca
       61 agcctccgca cggtcgcaac ctccacgcca cgcgacccgc gcgatcgacg ctcggtcgcg
      121 gagcctcgac gcggcacccc caacgactcg tgaacgggcg acggcggttc ggcccgcgtg
      181 actagcctaa agctggccgg tcacgccgac catgaccaga ttgcggctga actcgtcgtc
      241 cgggaaatcc gaccagcgtt gctggtagtc gtaaccagca ttgaccgaga aatatcgatt
      301 caagaagtat gtgactccgc cacccaccac gaccccgttg tcggtccggc tggggccatt
      361 gaagttgtcg cggatgtagt tgacgtgggc gccgaggtgc acgttgcgca gcagctcgtg
      421 gtcgacgctc gccccggccg tgctctgaaa gttcgactgg gtgtcgctgc cttccgagga
      481 ggtcggtaca aagttaccgg cgccagttgc cctgaccgtc gtcaattgcg tcggagtcca
      541 ggtcagatcg agcccgtaac cgacgccttc attgtcgctg aagctgccct cggcatagct
      601 ttgccggctg taaccgaccc gaatatcgct ggtaatcagg ttggtcagtt cgaccgcggc
      661 gccgacgccc gctccccatg tcttagtgtc acgttcgact ccgccagaat cgaccttctg
      721 atcgcgcttt ccaaccgcgt aggcgccgta gatataggtg ttgatccgcg gcgaaacgaa
      781 atagccggtg cgcagcccac cgatataatt attctggtcc cgatctgagt cttggtttgg
      841 ttcgtaatcg tcgcgggcca cagctccagt caccatgacg ttcagacggt tgaacagatg
      901 cgtgtagctg agggaaccgc catattgata gatctcagta gtatttttct ggttaggatt
      961 gttctccggg tcgtcccgac cctgatgcgc gcggccgaag ttcagaccgc cattgaaata
     1021 gttgtcgcga gtgacatcga gccggccgtt accttgaatg aacgcgtctt gatagttctc
     1081 atcggggtga tcggcgtaga agccgatgtt gccgccggcc gtcaagccga ggctgtgacg
     1141 actaaacgtg gattgcactt ggacccgcgg ctccagtacg gtgatgaagt cgtccttggt
     1201 attatgggga tcggcgaaca cgttgtcgtt gtattgttct gtgaggccga ggctcggaaa
     1261 gaccaggaag ctgccggccc ggatccctag cggctcgtag gggccgacct cctggccaaa
     1321 gcccagggaa ggagctccaa gggtgagcgc gacggcggtc gaaaccagaa gttggggcgg
     1381 gcagcgagcg atcacaaatc ctctcctcaa cgcggtggtc ggcggatgcg gatggcggtc
     1441 attgttaacc agtcccgcgt tatagccgtg gccacggcaa acgcaagacg ggccggattc
     1501 cgatcccagg atggctggcc gactggccag cggctgatcc gccgctcctc gtggctcggc
     1561 tcttgccaaa gccggcaaga ccagcgaggc tagacgccag ctgcggcggc aaggtcaaga
     1621 atccatgagc gcgggtcgcc tcgaccgttc cggatcatgc gaaaaccatt agctttgcca
     1681 acatgctatg gcaagtcgtt aattcctgag ttgactgtcg atggagatca gcccccgccg
     1741 ggcggccacc accgccaggc gagcggaccg tcgtcatcga agcgcgaagg ctgacccttg
     1801 ctgcgcagcc tgtttcgccg ccgactggaa ccctggccgt cggaaccaac ggtcggcggt
     1861 catccggcac gagtgccgcc gggcgtgtgc gtctatgcgg tcggcgacgt ccacggccgc
     1921 gccgatctgc tcgaagtcct gcaccgccgg atcgccgcgg atgcggacca gctgaccccg
     1981 ggtaccgaga agatcgtgct ctacgtcggc gactacgtcg accgggggtt ggagagccgc
     2041 caagtcctcg acgtgctgtt gagccggccg ctgccggact tccatgccgt ccacctgctg
     2101 ggcaatcacg acgcctggtt gctgagcttc ctgatcgatt ccaagatcgg cccgacgtgg
     2161 atgcggtttg gcggcgacgc cacgctccac agctatggga tccacctgcg accatcggtc
     2221 aacgacgtcc gctatttcga agatctccag gcggaattgc gcgaacgtct gccgcgccag
     2281 cacgtcgagt tccttgagga gctggagctg agctacgaga ccggcgacta cctgttcgtc
     2341 cacgccgggg tgcggccgaa ccagccgctc gaccggcaga tcgccgacga tctgctctgg
     2401 atccgcgaac cgttcctgag ctccaggcgc gatctcggca aagtcgtggt ccatggccac
     2461 acggtcgagg ccgagccagc ggtccgcgcc aatcgaatcg ggatcgacac cggcgcctgc
     2521 tggaccggtc gcctgacctg cctggtcctc gaggaaggcc gctatcgttt tctcaacacc
     2581 gccggtggca tcgttgctca gcaatgagct cggccacggg cgcggccgca tcgaggaggg
     2641 cgccgccgac ggcgcgttag cgggatgtcg aagccacgtg aaatcctggt caccggcggg
     2701 gcgggctaca tcggcagtca cgtgacgcgg cagctggtcg aggctggcga ccgggtggtg
     2761 gtctacgaca atctgtcgtc cggccatgcc tgggcggtgc tcggcgccga gctcgtggtc
     2821 ggcgatctcg ctgatcgcga gcgcctcgcg gcgtcgttcg gcggtcgcaa catcgatgcc
     2881 gtgttccatt ttgccgccca tatctgggtc acggaatcga tccgcgatcc ggtcaagtac
     2941 tatcggaaca acgtcgccgc ggctctcggg ctgttcgagg tcgctgccca gcatggggtc
     3001 cgccggatcg tgttctcgtc gaccgctgcg gtctacggcc aacccgattg tgctctgatc
     3061 gacgagggca tgccgctcgc gccgatcaat ccctatggcg cctccaagat gatggccgag
     3121 cggatcctga tggacatcgc cgcggcgtct gggctgcgct acgcgatcct gcgctatttc
     3181 aacgtcgccg gcgccgaccc gcagggccgg atcggcgagg cgacgccgga caaccagcat
     3241 ctgatcaagc ttgcctgcga gaccgccctc ggcgcgaggt cggcgatgcg catccatggc
     3301 accgattacc cgacgccgga cggcacctgc gtgcgcgact acatccacgt cgatgatttg
     3361 gcccgggcgc atctggcggc gcttgattat ctcgagcagg gcggcgactc gacggtgctc
     3421 aattgcggct atggccacgg cgcgagcgtc cggctggtgc tcgacacggt gcggcgcgtg
     3481 accgggatcg attttccgat cgagttcggc ccgcgccggc cgggcgaccc ggcggtgctg
     3541 gtggccagca atgacaaaat ccgccgcgtg ctcggatggc ggccgcgtca tgacgatctc
     3601 gagttcatcg tgcgcaccgc ctgggcctgg gagcagatcc ttcagagccg gcgcgccgac
     3661 gctatgccga cggccggctg attggcggag cggttgagca tcgataggcc ggccctttac
     3721 agcccgggcc tgggccgtag attggccagc acgtaaaccg gacggccggg agtgatccga
     3781 atcttcaagc cccgcggcct ggccgccgtg ctccctgcgc tgctgatgct cgcgtcggcg
     3841 gctctggcgc aggcggccgc accgtcggtc gggcctggat cgctccccgg gctcggcgcc
     3901 agtacgcccg cgccggaact cagccgcgag cggctccagg gcctgatcga cacgctcgag
     3961 gacccgggac gccgcgagca gctgatcgcg acgctgcgca cgctcgaggc cgcccaggct
     4021 gagcaacctc cgggcgagcc cttcctgtcg gacgacgtgg tcgcgggcgt tctgggtgag
     4081 atcacggccc gcaccgacgt ggtgcgcaag gtctcattgt cgattctgaa ttctctggac
     4141 cagattcctt tg
//
LOCUS       DATAOZ010000528         4240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_216123, whole genome shotgun sequence.
ACCESSION   DATAOZ010000528 DATAOZ010000000
VERSION     DATAOZ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216123"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..334
                     /locus_tag="VHR64_13445"
     CDS             143..334
                     /locus_tag="VHR64_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303884.1"
                     /translation="MTDRSAEDVEQRHRSLKHPDKPVDDATKAPSAPSYADESGATDL
                     GDDEPVVPEPVRGDTAEER"
     gene            370..1533
                     /locus_tag="VHR64_13450"
     CDS             370..1533
                     /locus_tag="VHR64_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX3303885.1"
                     /translation="MRIVEIREMAAPITSTIRNAYIDFSKMTVSVVAVITDQVRDGRT
                     VVGYGFNSNGRYAPSGLIRDRFIPRLMEAEPEALIADDGSNLDPHKIWSTMMSGEKPG
                     GHGERSVAVGTIDMAVWDAVAKIEEKPLYRLLADRYRDSRADERVFVYAAGGYYQPGK
                     DLHALQDEMRGYLDMGYSVVKMKIGGDTLTEDLRRIEAVIEVTGTGSKLAVDANGRFD
                     LDTAIAYAEALAPYGLFWYEEAGDPLDYALQAELANHYEHAMATGENLFSMQDARNLI
                     RYGGMRPDRDWLQFDCALSYGLVEYLRTLEMLDDHGWSARRVIPHGGHQMSLNMAAGL
                     GLGGNESYPGIFQPFGGFADGIAVEDGYVALPDIPGVGFEAKAELIALMRTLA"
     gene            1824..3080
                     /locus_tag="VHR64_13455"
     CDS             1824..3080
                     /locus_tag="VHR64_13455"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3303886.1"
                     /translation="MEPIRLGIIGCGGMGRRHLAGLGELARAGEHRIDLVAACDLNEQ
                     NARDLADEAFDLLGTRPTVFTNLTRMIQESDGLEAADCTTETGTHHAVAAGLLDLDLH
                     TMVEKPLGLTIRGCNHVIAAAERSGKILSVAENYRRDPINRLARALINDGAIGQSQFV
                     METAVRGRDDILITPWRHQKLTGTITLDAGVHNADILLYYLGDAVAAYGQTRLFHPTR
                     VRRDTAGPGGFYEKWAANLPKTVAATGEDAIFGMITFANGTLGQWVEYHAGHGEPFRH
                     RMVFGTSGSLAIPEDRTGKPIRLVLDDGTDVVDERVLDHAPGYRLDPAAARLFGGDRP
                     WTYDFDFVTTDRKLIALEYAELATCIRTGARPEVDGPAGRRAVALVHALFESHVAGRP
                     VTLAEIASGAVDAYQREIDEYYGLVG"
     gene            3114..3968
                     /locus_tag="VHR64_13460"
     CDS             3114..3968
                     /locus_tag="VHR64_13460"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3303887.1"
                     /translation="MRLAYSTWGMPTVPIEIAVAHCAALGFDGLELTVIPGWTTDAAT
                     LDSAARRRIRAVYDDHQIALCGLSGNVPLLESDGVLSEDAATRFCTYLDLAAELQHPG
                     ERLIVTTTSGGRSDQWDATKSRLVEALGALCSYAERVGVRVGIEPHVDAALHRPEQAR
                     WLLAQVDSPVLTIHFDISHFNVQGMDMETVITQLAPYSAHTHVKDERGIAPHHEFLIP
                     GEGDMDYVRYLQSMAANGYDGYIVVEISLMVQRRPDYDPLAAAATSYAVLSRAFQQAG
                     IKRPLVRW"
     gene            complement(4001..4150)
                     /locus_tag="VHR64_13465"
     CDS             complement(4001..4150)
                     /locus_tag="VHR64_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303888.1"
                     /translation="MNTEVQVVAWTRRYAFSLSPAEEGRASLSARPWDRAETRVQLPW
                     RSVFG"
BASE COUNT          764 a         1328 c         1383 g          765 t
ORIGIN      
        1 gcgggcgtca gacgaagtcc tatctgagca cgggatggta tgcaatggta ttgcgcgaca
       61 cttagatcct ggtgtccctt acccggcact actggttcgg ccttttgttg ctattcagcc
      121 gggcagattt tgggggagga caatgaccga tcggtccgcc gaggacgttg aacagcgtca
      181 ccgcagcctg aaacaccctg acaagccggt ggacgatgcc acaaaagcgc catcggcacc
      241 ctcatatgcc gacgaaagcg gtgcgaccga tctgggcgat gacgagcccg tggtcccgga
      301 accggtccgg ggtgataccg ctgaggagcg ctagctcgct ccccgaaggc actgggagaa
      361 acaacaccaa tgcgcatcgt cgagatccgg gagatggcgg cgccaatcac gtcgaccatc
      421 cgtaacgcct atatcgattt cagtaagatg acggtctcgg tcgtggcggt catcaccgac
      481 caggtgcgag atggccgaac cgtcgtcggg tatggcttca actcgaatgg cagatacgcc
      541 ccgagtggcc tgatccgcga ccggttcatt ccgcggctga tggaggcaga acccgaggcg
      601 ctgatcgccg acgacggctc gaatctggat ccgcacaaga tctggtccac gatgatgtcc
      661 ggcgagaaac ccggagggca cggcgagcgc tcggtggccg tcggcacgat cgatatggcc
      721 gtctgggacg cggtcgccaa gatcgaagag aagccgctct accggttgct ggcggaccgg
      781 tatcgcgaca gtcgggccga cgagcgggtc ttcgtctatg cggccggcgg ctactaccag
      841 ccgggcaagg atctgcacgc tctccaggat gagatgcgcg gctatctcga catggggtac
      901 tcggtcgtca agatgaagat cggcggcgac acgctcaccg aagatctccg ccggatcgaa
      961 gccgtgatcg aggtgaccgg caccggtagc aaactggccg tcgacgccaa cggccggttc
     1021 gaccttgaca cggccatcgc gtacgccgaa gcgctggctc cctatgggtt gttctggtac
     1081 gaggaggcgg gagacccgct cgattacgcg cttcaggctg agcttgcgaa ccactatgag
     1141 cacgcaatgg cgaccggcga gaacctcttt tcgatgcagg acgcgcgcaa cctgattcga
     1201 tacggcggca tgcgaccgga tcgggactgg ctccagttcg actgtgcgct gtcatatggg
     1261 ctggtcgaat acctgcgcac tctcgaaatg cttgacgacc atggctggag cgcgcgccgg
     1321 gttattccac atggcgggca tcagatgtcc ctgaatatgg cggccgggct gggattgggc
     1381 gggaatgagt cttaccccgg tatctttcag ccattcggcg gctttgccga cggcatcgca
     1441 gtcgaggatg ggtacgtcgc gttacccgac atccccggcg tcggtttcga ggcgaaggca
     1501 gagctgatcg ccctgatgcg gacgctggcc tgaccgaatc aagcggtgta gctatccctc
     1561 cagagctggc ttgagttcgt cgattctacg aaagcggcaa tcctgttcgg cgtgctgctt
     1621 tggcacgatt ttgacgaacc tctcatatgc cgtggcgcgg tgctccgtgc tcgatgttcc
     1681 ttgacccgtt tgcgtgctgg cgaccacctt ggcgacgcag cgtgggtggc cgcgcgttca
     1741 cggctgaacg ggcgattgtg cctggcggtc cgaatgccga caattgccgc catcacgcag
     1801 attgttgcgc gcgagggggc accgtggaac cgatacgact cggcatcatc ggctgtggtg
     1861 gcatgggccg gcggcacttg gctggcttgg gagagttggc gcgcgcgggt gagcaccgta
     1921 tcgatctcgt cgcggcctgc gatctgaacg agcagaacgc gcgcgacctg gccgacgagg
     1981 cgtttgacct gctcggaacg cgaccaaccg tgttcactaa tctgacccgg atgatccagg
     2041 agtcggatgg cctcgaggcc gccgattgca cgaccgagac cggcacgcat cacgcggtcg
     2101 ccgcaggtct gctcgatctc gacctgcata ccatggtcga gaaaccgctt ggcctcacga
     2161 tccggggctg caatcacgtg attgccgcgg ccgagcgcag cggcaagatc ctgtcggtgg
     2221 ccgagaacta ccggcgcgat ccgatcaacc ggctggcccg cgcgctgatc aacgatggcg
     2281 cgatcggcca atcccagttc gtcatggaga cggccgtccg cggtcgcgac gacatcctga
     2341 tcacgccctg gcgtcaccag aaactgaccg gcacgatcac gctcgatgcc ggcgtgcaca
     2401 acgcggatat cctgctctat tacctcggcg atgcggtcgc cgcctacggg caaacgcggc
     2461 tcttccatcc tacccgggtc cgccgcgaca cggccggacc gggcggcttc tacgagaagt
     2521 gggcggcgaa tctgccgaag acggtcgcgg ccactggtga ggatgccatc ttcggtatga
     2581 tcaccttcgc caatgggacg ctcgggcaat gggtcgagta tcacgcgggg cacggcgaac
     2641 cgttccgcca ccgcatggtg ttcggcacat ccggttctct ggcgatcccg gaggaccgga
     2701 ccggcaagcc cattcgcctt gtcctggatg acgggacgga tgtggtcgac gagcgggtgc
     2761 tcgaccacgc tccgggctac cggctggatc cggcggccgc cagactcttc ggcggagacc
     2821 gtccctggac ctacgacttc gatttcgtga ccactgatcg caagctgatc gcgctcgaat
     2881 atgcggagct ggcgacctgc atccgtaccg gtgccaggcc ggaggtggac ggaccggccg
     2941 gccgccgcgc ggtggcgctt gtgcatgcgc tcttcgagtc gcacgttgcg ggccgcccgg
     3001 ttacgctcgc cgaaatcgcg tccggcgcgg tcgacgccta ccagcgcgag atcgacgagt
     3061 actacggcct tgtgggataa cgccgaatca gtatcggagt gaggggcatc gggatgcgac
     3121 ttgcctacag cacctgggga atgccgacgg tcccgatcga gatcgcggtg gcccactgcg
     3181 cggcgctcgg gttcgacggg ctcgagctga ccgtcatccc aggctggacg accgatgccg
     3241 ccacacttga tagcgccgcc cggcgccgca tccgcgcggt ctatgacgac catcagatcg
     3301 cgctctgcgg cctatccgga aacgtgccgc tgctggagtc cgatggcgtg ctatccgagg
     3361 acgccgcgac tcgcttctgc acgtacctgg acctcgcggc cgagctccag catccgggcg
     3421 agcggctgat tgtcaccacc acctcgggcg gccgatccga ccagtgggat gcgacgaaat
     3481 cccggctcgt cgaggcgctc ggtgcgctct gctcctacgc agagcgggtt ggtgtccgag
     3541 tcgggatcga gccgcacgtc gacgcggcac tgcaccggcc ggaacaggcc cgctggctcc
     3601 tggcgcaggt cgattcaccg gtgctgacga tccatttcga tatcagccac ttcaatgtgc
     3661 agggcatgga tatggagacg gtgattaccc agctcgcacc gtatagcgcg cacacgcacg
     3721 tcaaggacga gcgtggcatc gcgccccatc acgagttcct gattcccggc gaaggcgaca
     3781 tggactatgt ccgctatctc cagtccatgg ccgccaatgg gtatgacggg tacatcgtgg
     3841 tcgagatcag cctgatggtc cagcgccgcc cggactacga tccactcgcc gccgcggcaa
     3901 cgtcctacgc ggtgctttcc cgggcctttc agcaagccgg catcaagcga cctctggtcc
     3961 gttggtgacg aacatagctg gcacagagag gtcatcagcc tcagccgaaa actgatcgcc
     4021 agggtagctg gacgcgtgtt tcggctcgat cccacgggcg cgcgctcagc gaggcgcgtc
     4081 cctcttcggc cggactcagg ctgaacgcat accgccgcgt ccaggcgacg acctggacct
     4141 ccgtgttcat cgcatcgacc tgatcggaag atcgcggcgg tattgactgg ctatccggtg
     4201 tagtttgcaa gagcgaatat cagtgctacg atagggttgt
//
LOCUS       DATAOZ010000529         8540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5208222, whole genome shotgun sequence.
ACCESSION   DATAOZ010000529 DATAOZ010000000
VERSION     DATAOZ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5208222"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="VHR64_13470"
     CDS             complement(<1..1460)
                     /locus_tag="VHR64_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3303889.1"
                     /translation="MSKLSSGKVRIIGLLALLAMLVPMFSTPVHSQDNGKILRIHQTV
                     DPDVVDPQKSSFTNEIELLSLNYEGLTKLDENLNTVPGAAESWEFNEDGTVLTFHLRQ
                     GLKYSDGSPLTAENYRYAIERTCDPNTAGQYQSILFEIKGCAEFAGSLTAPEVGEGTP
                     AAEVDPAAYETTKAALGAKVIDDNTLELTLTNPAPYYPTIASLWVFYPAKKELIEQGG
                     EDWWKDPTKQIGNGVFQMTDYAEDQQVSFVANENYWGGRAKLDGIDIVFQGESAVALE
                     AYRAGDLDIVQVDPQQIPEIQADPELNAQLVQYPVASTYVLNFNLTQKPFDDIKVRQA
                     FSQAFDRETYCAVVRNGDCTPTLTWIPAGLPGSIESDKYGFDPEAAKAALAESSYGGP
                     ENLPEIKLYYNSDDPANTARAEWVAGQYRDILGVTVTLQPTEGTALVALRKEPTTFPQ
                     MLLPGGWIQDYPDPQNWLSVYFKCDATFAKRFAYCNP"
     gene            1658..2908
                     /gene="glp"
                     /locus_tag="VHR64_13475"
     CDS             1658..2908
                     /gene="glp"
                     /locus_tag="VHR64_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX3303890.1"
                     /translation="MSKPNDLLHPREALQRVLLRCPSAKPETVELADATGRYLFDNVV
                     ARDDHPPFPASTMDGFAVVAADVSPWREITGEQAAGPQLPLEVSEGYAVKIMTGAPIP
                     AGADAVIPIEQVTLSEDHVVIEDVTLKPGLNIRPVGSDLARGQRVIESGTPIGSVELG
                     LIATMGYAEVSVARRPQVAVISTGDELVDPGADLAPGMIRDSNRFSLVAAIEAAGAEV
                     VHSSRVGDTVEELRRRLADLDGQIDVLVTSGGVSVGDRDVVKLLLGETANVHFRRVFM
                     KPGRPLTFATSGDLMIFGLPGNPVSSMVSFELFVRPALLSMMGATHPLRETTSVALAS
                     EIRPSDRIEFQRATITVAPNGRLKAETTGNQISSRLASLLGANGLLIIEPGTEPIAPG
                     QMVPAILIDIPKSTTPSPLAPSGS"
     gene            2938..3645
                     /locus_tag="VHR64_13480"
     CDS             2938..3645
                     /locus_tag="VHR64_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX3303891.1"
                     /translation="MSVTIHLRHFAIVREAVGAASETRTYPEPPTADAVFSELADAHP
                     ALAGLRRSIMVMVNQSYAGAETVLNDGDEVAFIPPVSGGAPAHLFRVTADPLDAREAE
                     QAVRDPGCGAVITFTGEVRDTARGQDVTALDYEAYAEAAERMMGTIAEEIEREFGVDR
                     IAIIHRVGLLMPGEASVVISVASPHRDEAFKAARHAIERLKQIVPIWKKEHYADGATW
                     IGSEADYQREIGRIPPS"
     gene            3744..4778
                     /gene="cysK"
                     /locus_tag="VHR64_13485"
     CDS             3744..4778
                     /gene="cysK"
                     /locus_tag="VHR64_13485"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX3303892.1"
                     /translation="MVTTASKAPDSIKNLTADKLVRRNRPGIAANATELIGNTPLVRL
                     SRFAPNTPGILVGKLESFTPGFSVKDRIAVAMIEDAEERGEITPGKTTLVEPTSGNTG
                     IGLAWVAAAKGYRLILTMPESMSLERRVLLLGYGAELILTPPSEGMAGAVRRAEQIAA
                     ETPDSWIPGQFTNPANPEIHRSTTAVEIWQDTDGAVDIVVSGIGTGGTITGVGQVLKQ
                     LKPGVKMVAVEPTESPLLSSGTFGPHKIQGLGANFVPDVLDTDIYDEIIHVSSEEAMA
                     AARELMRSEGLLKGISCGAAALAGRQVAAREENRGKLIVTVLPDTGERYLSTLLFQEL
                     REKAQSLTAS"
     gene            complement(4818..5567)
                     /locus_tag="VHR64_13490"
     CDS             complement(4818..5567)
                     /locus_tag="VHR64_13490"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX3303893.1"
                     /translation="MTISISIPESILTAYLRAGIANLPGGALTGNRSGFVIVETARIA
                     SLPDHAIHPDVGIVLRDRGVVALQSAVRPDELEQPTIWIRTSGPTAELLARATVAQYF
                     GFRPRVWVDESGAADADVVIVDEAAAIGPIESGYREDLTRAWFVLTGLPFVSHLLAVP
                     NGADPEQVAAITEWVGAGGGFDREQRRAVREEIAAVTGASLDSVVDLMTGIRGQLGLD
                     QRRSVAELFARSGVADQIGPVRWYRDENEPG"
     gene            complement(5564..6427)
                     /gene="pheA"
                     /locus_tag="VHR64_13495"
     CDS             complement(5564..6427)
                     /gene="pheA"
                     /locus_tag="VHR64_13495"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX3303894.1"
                     /translation="MVTAYLGPRGTFSEDAAISYAGPDGELLPMASIPALVAAVETGL
                     ANNAVLPIENSIEGAISTTLDLLIHETELSIIAELVVRVRLYLITVPGATLNDIAVVS
                     SHSNPLGQSRRFLERCLPKATQIAALSTAVAVQEVMEAGDPSRAAIGTLSAATMFGGQ
                     ILAHDIQDLQTNVTRFVALAASKAEPTGDDKTSIAFLLKANVPGALFRVLRPFAEAGI
                     QLTKLESRPSRTEILEYVFLCDFLGHRDDPTIAKALEAVAEECTMLKVFGSYPRFPLE
                     NMTFSSTGDRA"
     gene            6477..7124
                     /gene="thpR"
                     /locus_tag="VHR64_13500"
     CDS             6477..7124
                     /gene="thpR"
                     /locus_tag="VHR64_13500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX3303895.1"
                     /translation="MPREKHKFTKERPVDPDSVESQWRLFVAVDFPESRREVLEQLLE
                     TVRDANLPIRWIGANAAHLTLHFIGDVPVETAELLRLAFISAAGGTHPFSLKVDGAGA
                     FPNLNKPQIVWLGLAGDVASLRRLHRASETFLLGFDIVTEQREFKPHITLGRARQALQ
                     SPEINALVSLMRSDAIQAKLAELAEPFRVDHLTLYRSHLSHEGATYEPLATARFG"
     gene            7214..8314
                     /gene="prfA"
                     /locus_tag="VHR64_13505"
     CDS             7214..8314
                     /gene="prfA"
                     /locus_tag="VHR64_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX3303896.1"
                     /translation="MSAFLSTLAELKRRYDELDHQMADPAVLADPSKLAELGRERADL
                     EEVVSVYSDLQGSEQELKDAEELASDDDPELSGLAEDEIRRLKALREQYLTRLRTLLV
                     PKDPNDEKNVIVEIRAGTGGEEAALFAADLYRMYTRYAERHRWKIDVLSSTETDNGGF
                     REIIFEVSGKGAYSHLRYESGVHRVQRVPATESQGRIHTSTASVAVLPEAEEVDIKVN
                     DNDLRIDVYRSTGHGGQSVNTTDSAVRITHLPTGLVVTCQDEKSQLKNKLKAMTVLRS
                     RLYEIEQEKLSTERGDARRSQIGSGERSEKIRTYNFPQDRITDHRIKVNASNLPSVMD
                     GNIDIFVNELRAADEAERLRDAGLEDSPLAAD"
     gene            8326..>8540
                     /locus_tag="VHR64_13510"
     CDS             8326..>8540
                     /locus_tag="VHR64_13510"
                     /inference="COORDINATES: protein motif:HMM:NF037644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303897.1"
                     /translation="MTTIAETIRAAQRQLNQSSTPRLDAELLVGHALGLSRTQLLANL
                     RDPVPHESLDAIERFIERRANGEPVAY"
BASE COUNT         1602 a         2796 c         2665 g         1477 t
ORIGIN      
        1 ggattgcagt acgcgaaccg cttcgcgaag gtggcgtcgc acttgaagta gacgctcagc
       61 cagttctgcg ggtccgggta gtcctgaatc cagccgcccg gcagaagcat ctgcgggaag
      121 gtggtcggct ccttgcggag agcaacgagc gcggtgcctt cagtcggctg cagcgtgacg
      181 gtcacgccca ggatgtcgcg gtactgaccg gccacccact cggcgcgggc ggtgttcgcc
      241 gggtcatcgc tgttgtagta gagcttgatt tccggcaagt tctccggtcc accgtagctc
      301 gactcagcca gggcggcctt ggccgcttcc ggatcgaagc cgtacttgtc ggactcgatc
      361 gagcccggca gcccggccgg aatccaggtc agggtcggcg tgcaatcgcc attgcggacg
      421 acggcgcagt aggtctcgcg gtcgaatgcc tgcgagaagg cctgccggac cttgatgtcg
      481 tcgaacggct tctgggtcag gttgaagttg agcacatagg tcgatgcgac cgggtactga
      541 accaactgcg cattcagctc gggatcggcc tggatctccg ggatctgctg cggatcgacc
      601 tggacgatgt cgaggtcgcc ggcgcgatag gcctcgagcg cgaccgccga ctcgccctgg
      661 aagacgatat cgatgccgtc aagcttcgcc cgaccgcccc agtagttctc attggcaacg
      721 aagctgacct gctgatcttc ggcgtagtcg gtcatctgga agacgccgtt gccgatctgc
      781 ttggtcggat ccttccacca atcctcgccg ccctgctcga tcagctcctt cttggctggg
      841 tagaagaccc agagggaggc gatcgtcggg tagtacggcg ccgggttggt cagggtcagc
      901 tcgagcgtgt tgtcatcgat caccttggcg ccgagagccg ccttggtggt ctcgtacgcg
      961 gccggatcga cttcagctgc cggcgtgccc tcgcccactt ccggggcggt cagtgagccg
     1021 gcgaactcgg cgcaaccctt gatctcgaac aggatcgact ggtactggcc ggccgtgttc
     1081 gggtcgcagg tccgctcgat tgcataacgg tagttctcgg ccgtaagcgg cgagccatcg
     1141 ctgtacttga ggccctggcg gaggtggaag gtcaggacgg ttccatcctc attgaactcc
     1201 cagctctcgg ccgcacccgg aaccgtgttc aggttctcgt cgagcttggt caggccctcg
     1261 tagttgaggg aaaggagctc gatctcgttc gtgaaggaac tcttctgggg atcgaccacg
     1321 tccggatcga cagtctgatg gatccggaga atcttgccgt tgtcctgcga gtggaccggt
     1381 gttgagaaca tcggtaccag catggccaac agcgcgagca gcccgataat tcggaccttt
     1441 ccgctgctca atttcgacac aaacctacct cctgtacacc caaaccctcg caaacgcctt
     1501 cgtctgcgcg acaaaacgct acgccacgtt acgaatcagt gtcaactttt acgttttggc
     1561 actgggtatc acgtttcaat cggtcccgaa tcgggatacc ggcggagcgg ctactattca
     1621 atgattgact gtatctctga accgcgaggc aatcgatttg agcaaaccaa atgacctgct
     1681 gcacccgcga gaggcgcttc agcgcgtcct tctccgctgc ccgtcggcga agccggagac
     1741 ggttgagctt gcggatgcaa ctgggcggta cctgttcgat aatgttgttg cacgcgacga
     1801 ccatcctccg ttccccgctt cgacgatgga cgggttcgcg gtcgtcgcag cagatgtctc
     1861 tccgtggcgc gagatcaccg gcgagcaggc tgctgggccg caactgccgc tcgaagttag
     1921 cgagggatac gccgtcaaga tcatgaccgg cgctccgatc ccggctggcg ccgacgcggt
     1981 gatcccaatc gagcaggtca cgctctccga ggaccacgtc gtgatcgagg acgtcacact
     2041 caagcctggc ctgaacatcc gcccggtcgg ctcggatctg gcgcggggcc agcgcgtcat
     2101 cgaatcggga acaccgatcg ggtcggtcga gctcggactg atcgcgacga tgggctacgc
     2161 agaggtgagc gtcgcgcgcc ggccccaggt agccgtgatt tcgaccggtg atgagctggt
     2221 tgatcccggc gccgatctcg cgcccggcat gatccgcgac tccaaccgat tcagcctcgt
     2281 ggccgcgatc gaggctgccg gagccgaagt ggtccactct tcgagggtcg gagacaccgt
     2341 tgaagagctg cgtcgcaggc tggccgacct ggacggccag atcgacgtgc tggtcacgag
     2401 cggcggcgtc tcggtcggcg atcgcgacgt cgtcaagctg ctgctcggcg agacggccaa
     2461 tgtccatttc cggcgcgtct tcatgaagcc gggcaggccg ctcacatttg caacctcagg
     2521 cgatctgatg atcttcggcc tgccaggcaa tccggtctcg tcgatggttt cgttcgagct
     2581 cttcgtgcgg ccggcattgc tgtcgatgat gggcgctacg cacccactgc gcgagaccac
     2641 gtcggtcgct ctggcttccg agattcggcc gtcggatcgg atcgagtttc agcgcgcgac
     2701 catcaccgtc gccccgaacg gccgactcaa ggcagagacg accgggaacc agatttcgtc
     2761 gcgccttgcc tcactgctcg gagccaatgg actgctgatc atcgagccag gtaccgaacc
     2821 gattgcgcca ggacagatgg tgccagcgat cctgatcgat attccgaagt cgacaactcc
     2881 ctcgccgctc gccccatcag ggtcctgatc ggccaccatc caattagagg agttaccgtg
     2941 tctgtcacga ttcatcttcg ccacttcgcg atcgtccggg aggcggtcgg cgccgccagc
     3001 gaaacccgga cgtatcccga accgccgacg gccgatgcag tgttcagcga actcgccgac
     3061 gcccatccgg cgctggccgg gctgcgccgc tcgatcatgg tcatggtcaa ccagagttac
     3121 gccggcgcgg agacggtgct caacgacggc gacgaggtgg cgttcattcc gccggtctcc
     3181 ggcggtgcgc cggctcacct cttccgggtc acggccgatc cgctcgatgc ccgcgaggcc
     3241 gagcaagcgg tgcgggatcc tggttgcggt gcggtcatca ccttcaccgg cgaagtgcgc
     3301 gacacggcgc ggggccagga cgtgaccgca ctcgactacg aggcctatgc cgaagcggcc
     3361 gagcggatga tgggcacgat cgctgaagag atcgagcgcg agttcggggt cgatcggatc
     3421 gcgatcatcc atcgtgtcgg cctgctgatg ccgggcgagg cgagcgtcgt gatctccgtc
     3481 gcctcgccac atcgcgacga ggcattcaag gcggcccgcc acgcgatcga gcggctcaag
     3541 cagatcgtgc cgatctggaa gaaggagcac tacgccgacg gcgcgacctg gattggaagc
     3601 gaggccgatt accagcgcga gatcggccgg attccgccgt cctgacggcc attccggatt
     3661 cttgacaaag aactctacat atgccaacct cagcccctga caccgcgtgt cccacgcaag
     3721 gaagccgagg aaaggaatca cctgtggtaa ccaccgccag caaggccccg gattcaatca
     3781 agaacctgac cgccgacaaa ctcgttcgcc gcaaccggcc cggaatcgcc gcgaatgcga
     3841 cggaactaat cggcaacacc ccgctcgtcc gactctcccg attcgcgccg aacacccccg
     3901 gcatcctggt tggcaagctc gaatcattta cgcccggctt ttccgtgaaa gaccgcattg
     3961 cggttgcgat gattgaggac gccgaggagc gcggcgagat cacgccaggt aagacgacgc
     4021 tagtcgagcc gacaagcggc aacaccggga tcggccttgc gtgggtcgcg gcggccaagg
     4081 gctaccgcct aatcttgacg atgccggaga gcatgagcct cgagcggcgg gtgctgctcc
     4141 tgggttatgg cgccgagctg attttgacgc cgccctccga gggtatggcg ggcgccgtcc
     4201 gccgggcaga acagattgcc gccgaaacgc cggactcgtg gattcccggc caattcacca
     4261 acccggccaa cccggaaatt caccgtagca cgaccgcagt cgagatttgg caggataccg
     4321 acggcgcggt cgacatcgtt gtcagcggca tcggcaccgg cggcacaatc acaggtgtcg
     4381 gccaggtcct caagcagctc aagcccggcg tgaagatggt cgcagtcgag ccgacggagt
     4441 ccccgctgtt atcgagcggc acctttgggc cgcacaagat ccagggcctc ggggcgaact
     4501 tcgtgccgga tgtcctggac acggatatct acgacgaaat catccacgtg agcagcgagg
     4561 aggcgatggc agcggcccgc gagctcatgc ggtccgaggg actcctcaag ggcatctcct
     4621 gcggcgcagc ggccctcgcc ggccggcagg ttgcggcccg cgaggaaaat cgcggcaagc
     4681 tgatcgtcac cgtacttccg gataccggcg agcgctacct gagcacgctg ctcttccagg
     4741 aacttcgcga gaaggcgcag tcgctgacag ccagctaagc gtcgaccatt tccaaatctt
     4801 cgcgagccgg ggttccctta ccccggctcg ttttcgtccc gataccagcg caccggaccg
     4861 atctgatcag cgacgcccga cctggcgaaa agctcagcga ccgaccggcg ctggtcgagg
     4921 ccgagttgcc cgcgaatccc cgtcatcagg tcgaccacac tgtccagcga agcacccgta
     4981 accgcggcaa tctcctcccg gacggcccgg cgctgctccc tgtcgaaccc gccaccggcg
     5041 ccgacccatt cagtaatggc ggcgacctgc tcgggatcgg ccccattcgg caccgccagc
     5101 aagtgcgaca caaacggcag accggtcagg acgaaccagg cgcgcgtcaa atcctcccgg
     5161 taccccgatt cgatcggccc gatcgcggct gcctcgtcga cgatcacgac gtcggcatcg
     5221 gctgcgcccg actcatcgac ccacacgcgt ggccggaagc cgaagtactg ggcaaccgtc
     5281 gcccgggcga ggagctcagc ggtcgggccg gacgtgcgaa tccagatcgt cggctgctcc
     5341 agttcgtccg gccgcacggc cgattgcagg gcaacgactc cacgatcgcg caacacgatc
     5401 ccgacatccg gatgaattgc gtggtcgggt agcgatgcga tccgcgccgt ctcgacgatc
     5461 acgaagccgg atcgattccc ggtcaatgcc ccgccgggca agttcgcgat cccggcgcgc
     5521 agatatgccg tcaggatcga ctctgggatc gaaatcgaaa tggtcacgcg cggtccccgg
     5581 tcgagctgaa tgtcatgttc tcgaggggga agcgcgggta gctgccgaag accttgagca
     5641 tggtgcattc ctcggccacc gcttccaagg ccttggcgat ggtcgggtca tcccgatgcc
     5701 caaggaaatc gcacaggaag acgtactcga ggatctcggt ccgcgacggc cggctctcga
     5761 gtttcgtcaa ctggatcccg gcctcggcaa acggccgcag cacccggaac agcgcgccag
     5821 gtacattggc cttgagcaag aaggcgatcg aggtcttgtc gtcaccggtc ggctcggcct
     5881 tcgaagccgc cagcgcgacg aagcgggtca cgttcgtctg cagatcctgg atgtcgtggg
     5941 caaggatttg tccaccgaac atcgtcgccg cgctcaatgt tccgattgcc gcccgcgacg
     6001 gatcgcccgc ctccatgacc tcctgaacag cgaccgccgt gctgagggcc gcgatctgcg
     6061 tcgccttcgg caagcagcgt tcgagaaagc gacgactctg accgagcggg ttcgagtgcg
     6121 aggagacgac cgcgatgtcg ttcagcgtcg caccggggac cgtgatcagg tacagccgca
     6181 cccgcacgac cagctcggcg atgatgctca gctcagtctc gtgaatcaga aggtcgagcg
     6241 tcgtgctgat cgcgccttcg atcgagttct cgatcggcag aacggcgttg ttggcgagac
     6301 cggtctcgac tgcggcaacc agcgccggta tgctcgccat cggcagcagc tcgccatccg
     6361 gaccggcgta gctgatcgcc gcgtcctcgc tgaacgtgcc gcgtgggcct aaatatgccg
     6421 tcaccatcgt atcctcgtca cctcgtccga cgtcgtcgct ccgtatactg ccaatcatgc
     6481 cgcgagagaa acacaaattc acgaaagagc gcccggtcga ccccgattct gtcgagtcgc
     6541 aatggcgact cttcgttgcg gtcgacttcc cggaaagccg ccgagaagtc ctcgagcagc
     6601 ttcttgaaac ggttcgcgac gccaatctcc cgatccggtg gatcggcgcc aacgccgcgc
     6661 acctgacgct gcatttcatc ggcgacgtgc cggtcgagac cgccgagctg ctgcggctcg
     6721 ccttcatctc agcggcaggc gggacgcatc cgttctcgct caaggtcgat ggcgcaggcg
     6781 ccttcccgaa cctgaacaag ccgcagattg tctggctcgg cctggccgga gacgtcgcat
     6841 cgctgcggcg cctccatcgt gccagcgaga cattcctgct cggcttcgac atcgtgaccg
     6901 agcagcggga gttcaagcca cacatcacgc tcggccgtgc ccgtcaggcc ttgcaatcgc
     6961 cggagatcaa cgcactggtc agtctgatgc ggtcggacgc gatccaggcc aaactggccg
     7021 agctagccga gccgttccgt gtcgaccacc tcaccctcta ccgcagccac ctctcccatg
     7081 aaggggcaac ctatgaaccg ctcgccactg ccaggttcgg ttgaacggtg cggagcaccg
     7141 gtcgtttatc attagggtga tctgcgcgtg cgccagatcg cactccgtac atccaacacg
     7201 aaaccactga accatgagcg cattcctgag cacactggcg gagctgaaac gccggtacga
     7261 cgaacttgat catcagatgg cggatccggc tgtcctggcc gatcccagca agctggccga
     7321 gcttggtcgc gagcgagccg acctcgagga ggttgtctcg gtctactctg atttgcaggg
     7381 cagcgagcag gaactcaagg atgccgagga gctggcgtcg gacgacgatc ctgagttgtc
     7441 gggcctggcc gaggacgaaa tccggcggct gaaggccctc cgcgagcagt acctcacccg
     7501 cctgcgcacc ctgctggtgc cgaaggaccc gaacgacgag aaaaatgtga tcgtcgagat
     7561 ccgggccggg accggcggcg aagaggcggc cctcttcgcc gccgatctct accgcatgta
     7621 cacgcgctat gccgagcggc atcgctggaa gatcgatgtt ctctccagca ccgagaccga
     7681 caatggcggc tttcgggaga tcatcttcga ggtctccggc aagggcgcct atagccatct
     7741 gaggtacgag agcggagtcc accgcgtgca gcgggtcccg gccaccgaaa gccagggccg
     7801 gatccacacc tcgaccgcca gcgtggccgt tttgcccgag gcggaagagg tcgatatcaa
     7861 ggtcaacgac aatgatctcc gcatcgatgt ctaccgctca accggtcacg gcggccaaag
     7921 cgtcaacacg accgactccg ccgtgcgcat cacgcacctg ccgacgggtc tggtcgtgac
     7981 ctgccaggac gagaagagcc agctcaaaaa caagctcaag gcgatgactg tcctgcgtag
     8041 ccggctctac gagatagagc aggagaagtt gtccaccgaa cgtggcgacg cgcgccgctc
     8101 ccagatcggc agcggagagc gcagcgagaa gatccggacc tacaacttcc cgcaggaccg
     8161 cattacggat caccggatca aggtcaacgc cagcaacctg ccctcagtca tggacggcaa
     8221 catcgacatt ttcgtgaacg agctgcgggc ggccgacgag gcggagcgac tccgcgatgc
     8281 cggcctcgag gacagtccgc tcgcggccga ctgatcgacg ccggcgtgac cacaatcgcc
     8341 gagaccatcc gggcggcgca gcggcaactg aaccaaagca gcacgccgcg cctcgatgcc
     8401 gagctgctcg tcggtcatgc actcggcctg tcgcgtaccc agcttctggc taacctccgc
     8461 gatccagttc cgcacgagtc gctcgacgcc atcgaacgat ttatcgagcg ccgggcgaac
     8521 ggcgagccgg tcgcctacat
//
LOCUS       DATAOZ010000530         9150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_736301, whole genome shotgun sequence.
ACCESSION   DATAOZ010000530 DATAOZ010000000
VERSION     DATAOZ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736301"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..1707
                     /locus_tag="VHR64_13515"
     CDS             193..1707
                     /locus_tag="VHR64_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3303898.1"
                     /translation="MRVTREGPVPIARQIEQAIGELIASGEFAPGQRLPTEMELCERL
                     GVSRTPVRQALGKLVARGLLVRQAGRGTFVTVNGIPSAATESLIEDITVTVPEERWCW
                     PMQQAALSWNSENPDRPVRLRFQMTEQSELRERLMHAVAQGTAADVALLDSVWVAEFA
                     ERGYLRPLASIDPAIAGALVADLVPPLLAENSVRGELFALPGDADFALLWYRKDWFTA
                     EGLAPPRTWDDWLACARHFQRAAVRDRYGLDLYPLAFAAGATAGETSAYQLLPVLWSV
                     GADVIANHQVVLNSRATRAAVAFIAGLVQKHGIAARSVVTAPWNGPALALAAGSVAMS
                     LGGSYEGALIRAAAGWTETEFLARVGFVPIPAGPGGSQATLLGGLSYGVFRQSQRSER
                     ALALLDRAFRPDILRVFCAQTGQNPPTISATRALEAETEPFLHATAQLLPHARSRWPI
                     AEYARVSAQLVRMFESAILAEATPEEAVARAAAIIAGITGLPERTRRGPAWPAA"
     gene            complement(1900..4242)
                     /locus_tag="VHR64_13520"
     CDS             complement(1900..4242)
                     /locus_tag="VHR64_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303899.1"
                     /translation="MRWPWRDRQTNDARALDGYWDEVVRAAPAMPVPQPDKSPELARI
                     IRQLHAAEVADRSRPAYEDRLLQRLLAFQESATADTTLTIAPALSSPPIRRDSRVDWR
                     SPSGRRIGTRLSGAAAFAVVLIVVLVGTQFANTRWHREERPAVVSSPPASPTIVASPG
                     EIPELTGVSLLWSAQDEQTRLAEMADVAVAPNGDIYAVDCKNELILVFSADGTYLESW
                     GGAGTEPGQFNFGNCTFDTSSEGLANLLGAIGIDRSGNIYVFDSMNNRVQKFGPDHSF
                     ITQWGTYGSGIGQFNRTLGTVDAAHDLVYVADLNNQRIQVFDLDGNFIRSWKTSTETL
                     PDGFGPFDVAVDSAGNVYVSELDLHMVQKFDPTGKLLGVVVPKDGPGRSFGIALDAHD
                     NLYVSFHDAAVIDVYRPDGTAIGQISTAGDGRAFRLPGGLAFDGDGNLLVASGGGRNK
                     LVKLSVPGDLNRREQPAVIAPSTPVATPGATPKSAEVPIITGVKVVWSLANSDDPLSR
                     PGGLGIAQDGTISVGDVKAQTINRFGPDGTYQSSWGAPGKDPGQFDFADPLYPNQFFF
                     SPISFDSDGNMYVFDSFNDRIQKFNSSGEFVTQWGEPGHGDGQFDIPLGTVDAKSGRV
                     YVADSRNNRVRVFDVDGTFLFGFGTEGTGEGQFTEPIAIAFGPDGNVYVGEGRGHRIQ
                     VFDPDGKFLRMFDSGEATGSIRALAFDSNGNLYAALHDQSMVRVYTPDGAVIGQFHAL
                     DDGGTLFRPVDLTFTADGHMIIACESGPNRLVEVEMPAFE"
     gene            complement(4243..4851)
                     /locus_tag="VHR64_13525"
     CDS             complement(4243..4851)
                     /locus_tag="VHR64_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3303900.1"
                     /translation="MTEREDITAMRARDGRHLEPLAWAESDELVAARAAADPAAMTEL
                     YRRYIARVDRYCRRRLPNPELVEDVTSQIFLKVLEGLRKRRVENVASWIFTIAHNEVV
                     SQYRRRRELVGITEAVLALGDDDQLEDTVITHVVAGDLRAMLSLLSADQQRVLELRLA
                     GLTSREIRQVLGRSQTWVGTTEYRAVHRLRDLMTPNHPGEER"
     gene            5151..7460
                     /locus_tag="VHR64_13530"
     CDS             5151..7460
                     /locus_tag="VHR64_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3303901.1"
                     /translation="MTTAPRRFQPAPLPTPRTPLIGRERELADACRLLADSATSLVTL
                     TGPGGIGKTRLALAVAHKPQPRFGDGVAFVRLAQLRDSTLVLPTIAEMLSVPETGAAP
                     IADLVRDAIGNSHLLLVLDNFEHVTVAAMDLGTLLDTCPNLTVLVTSRVPLRVSGEQI
                     YPVPPLAIPQSSRMTSRDVTESEAGRLFALRAAAVRPGFAINAENAADVAAICQHLEG
                     LPLAIELAAARINILSPAALLARLDRRLPLLTGGPRDLPERQQTMHNAIAWSYDLLPK
                     MEQRLFRRLAVFDGGFSLDAAEAITAGDSTVDALAGVSALADASLIRPIDDPGSEPRF
                     QMLETIREFGLERLAETGDESATRERHARYILQLVRTGNDLRDTPRASDWLVRLTSEI
                     ANIRAARSWFENTGDAPAGLALADGASWIWDWRTLFVEGRDWLRQSLFAAPDAPPGVR
                     ASAMVGAGWMAYRQLDFADAEQNAITGLTLAQAACDHRIAAELLDVLGKVALETGRIQ
                     DARARFEEMLALTRAHRLDHYESAAINSLGIVAFTMGELAEAQHHHEASVAIDRRIGH
                     RLSLANHLFALAMTTYCQGKLERTAEFLQEQLRAIVDLDLGIEFGCQIVAMFAAASGQ
                     PGGAARLFGASEATADQLGADLFETDAYRPWYESAVASAREALGPDAFETAMRAGREF
                     SRAQTLDEAMAVFAPPPRDEATSAFDLSKRERDVLHLLAQGHSNRAIADQLFIAEATV
                     KVHVTSIFTKLGVDSRSAATAVAIRKGLA"
     gene            complement(7533..7928)
                     /locus_tag="VHR64_13535"
     CDS             complement(7533..7928)
                     /locus_tag="VHR64_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303902.1"
                     /translation="MFEIVEDDEEMPVADVIPDKFGERRCSGFPDAERLRQSGEYKRW
                     VPQWGQSNEQNAVTECGLYGTSSRHSEAALPDTTGASQGHEGDVGIAKETAKIGQLSL
                     AADERRARRGQRGELASSRSLAHGVYLVM"
     gene            7989..>9150
                     /locus_tag="VHR64_13540"
     CDS             7989..>9150
                     /locus_tag="VHR64_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303903.1"
                     /translation="MVTSRVPLRLSGEQNYPVPPLAMPETAKTAREHVAESEAGRLFA
                     ARAAAVQPTFAIRDDNAADIATICQRLDGLPLAIELAAARANVLTPEALLARLDRRLP
                     LLTSGARDLPERQQTMRQTIAWSYDLLPEPDPRLFRWLSVFDGGFSLDSAEAIVADDP
                     KLDAFAGVSALVDNSMIRQVEGPAGEPRFLMLETLREFGLEQMAAAGEEAAAREAHAA
                     HYLTLAERSYQELGGSRSGYWHAKWVAEIDNVRAAITWLGESGAAASGMRLVYSLWQL
                     WDHTGRQREPVETVERLLAAGPDAPPAVRGGAMIIAGWALLHLQDLAKAAQFAETALS
                     LARQGQDARILMLAYEFMGAVRGVSGDLADGARYTQESLELARAHGEDSIANG"
BASE COUNT         1573 a         3145 c         2796 g         1636 t
ORIGIN      
        1 gattccatcc tgactgagcg gccccatttt ggccctggtt caggctgaat tcaggacaca
       61 cgctggcgat tccagtcgtt tctggcaggt acttccaatt ccggtgttcg aaatgtacta
      121 tccagacacc gctctccggc gcccgatcaa cccctgaccg atgcgaccgc cgcagcgcgc
      181 agagtgtccg cgatgagagt aaccagggaa gggccggttc caatagcccg ccagatcgag
      241 caagccatcg gcgagctgat cgccagcggc gagttcgcac cgggccaacg cctgccgacc
      301 gagatggagc tctgcgaacg actcggcgtc agccgcaccc ccgtccgcca agccctcggc
      361 aagctcgtcg cccgtggcct gctcgtccgc caggccggac gcggcacctt cgtcacggtc
      421 aacgggatcc cctcggcggc aaccgagagc ctcatcgaag acatcacggt cacggtcccg
      481 gaagagcgct ggtgctggcc catgcagcag gccgctctct cctggaatag cgaaaacccg
      541 gaccgccccg tgcgccttcg gtttcagatg accgagcaat ccgagctccg cgaacggctg
      601 atgcacgcgg tcgcccaggg gaccgccgcc gacgtggccc tcctcgactc ggtttgggtt
      661 gccgagttcg ccgagcgcgg ctacctccgg cccttggcct cgatcgaccc ggccatcgcc
      721 ggcgccctgg tcgccgatct cgtccccccg ctgctggccg agaattctgt ccggggcgag
      781 ctcttcgccc tccccggcga tgccgacttc gctctgctct ggtatcggaa ggactggttc
      841 accgccgaag ggctggctcc gccccggacc tgggacgact ggcttgcctg cgcccgccat
      901 ttccagcgcg ccgccgtgcg cgaccggtac gggctcgacc tctacccgct ggccttcgcc
      961 gccggcgcca ccgcgggcga aacctcggcc taccagttgc tgcctgtgct ctggtccgtc
     1021 ggcgccgacg tgatcgccaa tcaccaggtg gtgctcaatt cccgcgccac ccgcgccgcc
     1081 gtcgccttca tcgccggcct ggtccagaag cacggaatag ccgcccgatc ggtcgtcacc
     1141 gctccgtgga acggacccgc gctcgccctg gcggccggct cggtcgcgat gtcgctgggc
     1201 gggagctacg aaggcgcgct gatccgagcc gccgccggct ggaccgagac cgaattcctg
     1261 gcccgcgtcg gattcgtgcc gatcccggcc ggacccggcg gaagccaggc caccctgctc
     1321 ggcggactca gttacggcgt cttccgccag tcgcagcgct cggagcgagc cctggccctg
     1381 ctcgaccgcg ccttccgacc ggatatcctg cgcgtcttct gcgcccagac cggccagaac
     1441 ccaccgacca tctccgcaac ccgcgccctg gaagcggaga ccgagccctt cctccatgcc
     1501 accgcccagc tcctgccgca cgcccgttcc cgctggccga tcgccgaata cgcgcgcgtc
     1561 tcggcccagt tggtccgcat gttcgagagc gccatcctcg ccgaagccac gcccgaggaa
     1621 gccgtcgccc gcgccgccgc catcattgcc ggcatcaccg gcctccccga acgcacccgc
     1681 cggggccccg cctggcccgc cgcctgacag ccgaatcgaa aactgaggca atggctaaag
     1741 cggagcggca ccgcgcggcg ggcaattacc gctgcccggg gtcagtcggc acgctcagct
     1801 tgatcagctt gttgcgaccg ctagcgcttg ccaccaccag attgccatcg ccgtcgaagg
     1861 ccacacctcc gggctgcctg aacgctcgtg agacatcgat tattcgaagg caggcatctc
     1921 gacctcgacc aaccggttcg gtccgctttc gcacgcgatg atcatgtgcc cgtccgccgt
     1981 gaacgtcagg tcaaccggcc ggaaaagcgt gccgccgtcg tccagcgcgt ggaattggcc
     2041 gatcaccgcc ccgtcaggcg tgtagacacg gaccatggac tgatcgtgta gcgcggcgta
     2101 gagattgccg ttggaatcga acgcgagcgc gcggattgac cccgttgctt cgcccgaatc
     2161 gaacatgcgc aggaatttcc cgtccggatc gaatacctgg atgcgatgac cccggccttc
     2221 acccacgtag acgttgccgt caggtccgaa tgcgatcgcg atcggctccg tgaactgacc
     2281 ttcgcctgta ccttcggtgc cgaacccgaa caggaacgtc ccgtccacgt cgaatacccg
     2341 aacccggtta ttccgggagt cggccacgta cactcgcccg gatttggcat cgacggtacc
     2401 gagcgggata tcgaactggc catcaccgtg gccgggctcg ccccattggg tgacgaactc
     2461 gccggagctg ttgaatttct ggattcggtc gttgaagctg tcgaacacgt acatgttgcc
     2521 gtccgaatcg aacgagatcg gcgagaagaa gaattggttc gggtaaagcg ggtcggcgaa
     2581 atcgaactgg ccgggatcct tccccggcgc gccccagctg gactggtacg tcccgtccgg
     2641 gccgaatcgg ttgatcgtct gcgccttgac gtcaccgacg gagatcgtcc cgtcctgcgc
     2701 aatccccaat ccacccggtc gagaaagcgg gtcgtccgaa ttcgcgaggc tccagactac
     2761 cttcaccccc gtgataatcg ggacctcggc tgacttcggc gttgccccag gagtggccac
     2821 tggcgtcgag ggggcaatca cggcaggttg ctcccggcga ttcaaatcgc cgggcacgct
     2881 caatttgacc agcttgttgc gacccccacc acttgcgacc agcaggttgc catcgccgtc
     2941 gaaggccaaa ccgcccggta acctgaacgc tctgccatcg ccggccgtgc tgatctggcc
     3001 aatcgccgtc ccgtccggtc ggtagacatc gatcacggcc gcgtcatgaa acgacacata
     3061 caggttgtca tgcgcatcga gggcgattcc gaacgaccgc ccaggtccat ctttcggcac
     3121 gaccacgccg agtaatttcc cggtcggatc gaacttctgg accatgtgca ggtccaactc
     3181 ggaaacatag acgttgccgg ccgagtcgac cgcgacgtca aatggtccga aaccgtccgg
     3241 aagcgtctcg gtcgatgtct tccagctccg tatgaagttc ccatcaagat cgaacacctg
     3301 gatccgctgg ttgttcaggt cggcgacgta gaccagatcg tgcgccgcgt cgaccgttcc
     3361 gagcgtccgg ttgaattggc caatcccgga accgtacgtc ccccattgcg ttatgaacga
     3421 gtggtccggt ccgaacttct gaacccggtt gttcatcgag tcgaagacgt agatgttccc
     3481 gctccggtcg attccgatcg ccccgagcaa attggccaga ccttcagaag aagtatcgaa
     3541 agtgcagtta ccgaaattga attgccccgg ttctgtgccc gccccgcccc aactctccag
     3601 gtacgtgccg tccgccgaaa agaccaggat cagctcgttc ttgcaatcaa ccgcatagat
     3661 gtcgccgttg ggcgctacgg ccacatcggc catctctgcc aggcgtgtct gttcgtcttg
     3721 tgcgctccac agcagggata cgccggtcaa ctccggaatc tcgcccggac tcgcaactat
     3781 ggtcggcgaa gctgggggcg acgaaacgac ggccgggcgt tcctcccgat gccagcgagt
     3841 gtttgcgaat tgcgtgccga ccagaaccac gatcagaacc accgcgaacg cggcggcccc
     3901 ggaaagccgg gtgccgattc tccgcccgga gggagatcgc cagtcgactc gtgaatctcg
     3961 cctgatcgga ggggatgaca aggctggtgc gatggtgagc gtcgtatccg cagtggcgct
     4021 ctcctgaaaa gccaggaggc gctgcagcag ccgatcctcg taagcgggac ggctgcgatc
     4081 agccacttcg gccgcgtgca gttgacgaat gattcgagcc agctccgggg atttgtccgg
     4141 ctgcggcacc ggcattgccg gcgcagcgcg aacgacctcg tcccagtacc cgtcaagggc
     4201 gcgcgcgtcg ttggtttgcc tgtctcgcca cggccagcgc atctagcgct cctctccggg
     4261 atggttgggt gtcatcagat cgcgcaggcg gtgcaccgcc cggtactctg tcgttccgac
     4321 ccaggtctgg cttctcccga gcacctgccg gatctcgcga ctggttaacc ccgccagccg
     4381 cagctcgagc acgcgctgct ggtccgcgct cagcagcgac agcattgccc gcagatctcc
     4441 ggcaacgaca tgggtgatga cggtgtcttc gagttgatcg tcatcaccaa gagcgagtac
     4501 tgcctcggtg atcccgacaa gttcacgtcg gcgccggtat tggctgacca cttcgttgtg
     4561 ggcaatcgtg aatatccatg acgcaacgtt ctcgacccgg cgcttgcgca gaccctcgag
     4621 caccttcagg aagatctggc tggtaacgtc ctccaccagc tccgggttcg gcaggcgccg
     4681 gcgacagtag cggtcaaccc gggcgatgta tcgccggtag agctcggtca tcgcggccgg
     4741 atcggcggcg gcgcgggccg ccaccaactc gtcggattcc gcccatgcca gcggctcgag
     4801 atgtcgtcca tcccgggccc gcatggctgt gatgtcctcg cgctccgtca tgccgtctcc
     4861 cctgcataca ggtagacgca gcagtgggga tttgtgcaca gattcaggcg ctccgtgtgc
     4921 gagagcgcag tcattgcgat gtggttccag taagtaacga tcatagcggg aggggcaatt
     4981 gggagtcacc ggcgggccgc gccgaggaac agacgtggtg gagcatgaag atcgccacgg
     5041 acgaggtgga cgcgcagccg ggtcatagcg gcgcctcagt ggtgcttgcg gcatatttgc
     5101 ctacgttgac caacccgcta tgatcgaagc cgcatcgaca ggtcaccatc atgacgacgg
     5161 ctccgcgacg ctttcaacct gcgcccttgc cgactccccg cacgccgctg atcggccgtg
     5221 agcgagaact ggctgatgcc tgccgcctcc tcgccgattc tgcaacttcc ctcgtcaccc
     5281 tcaccggccc tggcggtatc ggcaagacgc ggctggccct ggccgtcgcc cacaagcccc
     5341 agccgcgttt cggggacggc gtggcgttcg tacggcttgc ccagttgcga gattccacac
     5401 tcgtgctccc cacgattgcg gagatgttga gcgtgcccga aactggagcc gccccgatcg
     5461 ccgaccttgt gcgggacgcc atcggcaata gtcacctgct gctcgtcctg gacaatttcg
     5521 agcatgtcac cgtcgccgca atggacctcg gcacgttgct tgatacctgc ccgaacctca
     5581 cagtccttgt tacaagccgt gtcccacttc gtgtgtccgg cgagcagatc tatccggtcc
     5641 cgccactggc gataccccag tcatcccgca tgacgagtcg cgatgtgacc gagtctgagg
     5701 cgggaaggct cttcgccttg agagcggccg cggtccggcc cgggtttgcg atcaatgctg
     5761 agaacgcggc ggacgtagcg gcgatttgcc agcacctgga ggggcttccg ctcgcaatcg
     5821 aactggccgc cgcacgcatc aacatcctga gtcctgcagc cctgctcgcc cgcctggatc
     5881 gccgcctgcc gctcctgacc ggtggtcccc gcgacctacc cgagcgccag cagacgatgc
     5941 acaatgccat cgcctggagc tacgacttgc tcccgaagat ggagcagcgt ctgtttcgcc
     6001 gcctggcggt cttcgacggt ggattcagtc tcgatgcggc agaggccatc acggccggtg
     6061 attcaacagt cgatgccctc gccggggtaa gtgcgctggc cgatgccagc ctgatccggc
     6121 cgattgacga tccgggcagc gaaccgcgct tccagatgct cgaaacgatc cgcgagttcg
     6181 gcctcgagcg cttagccgaa accggcgatg agagtgccac gcgtgaacgg cacgcccggt
     6241 acatcctcca gttggtccgg accggaaacg acctccgcga taccccacga gcaagtgatt
     6301 ggcttgtgcg ccttaccagc gagattgcga acattcgggc cgcgcgttcc tggtttgaaa
     6361 acactggaga tgcccccgct ggtttggcgc ttgcggacgg cgcatcctgg atttgggact
     6421 ggcgcaccct ctttgtcgaa ggtcgggatt ggctgagaca aagcttgttt gccgccccag
     6481 acgctccacc cggagtgcgt gcctccgcca tggtcggggc cggctggatg gcctatcgcc
     6541 aattggactt tgccgatgcc gaacagaatg ccatcaccgg gctcacgttg gctcaagccg
     6601 cctgcgatca ccgaattgcg gccgagctcc tggacgtgct cgggaaagtc gcgcttgaaa
     6661 cgggccgaat ccaggatgcc cgtgcgcgct tcgaggaaat gctggcgctg acgcgagctc
     6721 acaggttgga tcactatgag tcggcagcca tcaacagctt gggcatcgtc gcgttcacga
     6781 tgggcgagct cgccgaagcc cagcatcacc acgaggcatc cgtggcaatc gaccgacgca
     6841 ttggccaccg cctcagcctc gccaatcacc tgtttgcgct ggcaatgacc acatattgcc
     6901 agggtaagct cgaaaggaca gctgagttcc tgcaggaaca gttgcgggca atcgtcgatc
     6961 ttgacctggg catcgaattt ggctgccaga tcgtggcgat gttcgccgct gccagcgggc
     7021 agccaggcgg tgcggctcgc ttgttcggag caagtgaggc gacggctgat cagctcgggg
     7081 ccgatctgtt tgagactgac gcctaccgtc cctggtacga atctgccgtg gccagcgccc
     7141 gcgaggcgct tggccccgat gcgttcgaga ccgccatgcg ggccggacgg gaattctccc
     7201 gcgcgcagac gctcgacgag gcaatggctg tcttcgctcc gccacccagg gacgaggcca
     7261 ccagcgcctt cgatctgagc aagcgcgagc gcgacgtcct ccacctgctc gcccagggac
     7321 actccaatcg ggccattgcc gatcagctct tcattgcgga ggccaccgtc aaggtccacg
     7381 tcacgtcgat cttcaccaag ctcggcgtcg actcccgctc cgccgccacc gccgtcgcca
     7441 tccgcaaggg actggcctag gtgggtccct cgctgccaga cccacatcgc gcccccgatc
     7501 ccggctcgtt gacgctgccg gtatgatcga catcacatca ccaggtagac accatgagcc
     7561 aggctccgcg acgacgccaa ctcgccccgt tgcccacgcc tcgcacgccg ctcatcggcc
     7621 gcgagcgaga gttgaccgat ctttgccgtc tccttggcaa tcccgacatc cccctcgtga
     7681 ccttgactgg ccccggtggt gtcgggaaga gccgcctcgc tgtggcggct gctcgtgcca
     7741 tagagcccac attcggtgac ggcgttttgt tcgttcgact ggccccattg cgggacccaa
     7801 cgcttgtatt ccccgctttg gcgcaagcgc tcggcgtccg ggaagccgga gcaacgccgc
     7861 tccccgaact tatccgggat gacatcggcg accggcatct cctcgtcgtc ctcgacaatt
     7921 tcgaacacgt caccgaggcg gccgccgaac tgggtatcct cctcgccgaa tgtccccggc
     7981 tcacggtcat ggtgacgagc cgggttcccc tacgcctctc cggcgagcag aactatccgg
     8041 tcccaccgct ggctatgccg gagacagcga aaacagcgcg ggaacacgtc gccgagtctg
     8101 aagcagggag gctcttcgcc gcccgtgccg ctgccgttca gcccacgttc gccattcggg
     8161 acgataatgc cgcggacatc gcgacgattt gtcaacgact ggatgggttg cccctcgcaa
     8221 tcgaacttgc cgccgctcgt gcaaacgtcc tgaccccgga agcgcttctc gcccgcctgg
     8281 atcgccgctt gcccctcctg accagcggcg cgcgcgacct gcctgagcgg cagcaaacga
     8341 tgcggcagac gatcgcctgg agctacgacc tgctgccgga accggaccca cgcctgtttc
     8401 ggtggttgtc cgtcttcgac ggcggcttca gcctggactc ggccgaggcc atcgttgcgg
     8461 acgatccaaa gctcgacgca ttcgcgggtg tctccgcgct ggtcgacaac agcatgattc
     8521 gtcaggtcga gggtccggcc ggcgagcccc gcttcctgat gctcgagaca ttgcgcgaat
     8581 tcgggcttga gcagatggcc gcggccgggg aagaggccgc cgcccgggag gcccacgccg
     8641 cccactatct gacgctggcc gaacggtctt accaggagtt gggcggctca cggagcgggt
     8701 attggcacgc caagtgggtc gccgagatcg acaacgttcg cgccgccatt acctggctag
     8761 gtgagagcgg agcagccgca tcgggaatgc gcctggtcta ctctctatgg cagctttggg
     8821 accataccgg tcgccaacgc gaaccagtcg aaacggtcga gcgattgctg gccgcgggcc
     8881 cggacgcgcc gcccgccgtg cgcgggggcg cgatgatcat tgctgggtgg gcactgctcc
     8941 atctccagga tctcgcgaag gcggcgcaat ttgcggaaac cgcgctttcc ctggcgcggc
     9001 agggccaaga cgccagaata ctcatgttgg cgtatgagtt catgggtgca gtgcgcggcg
     9061 tctcaggtga cttggctgat ggtgcgcggt acacgcagga atcattggaa ctcgcccgcg
     9121 cccatggcga ggactcgata gccaatggtg
//
LOCUS       DATAOZ010000531         3764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4419297, whole genome shotgun sequence.
ACCESSION   DATAOZ010000531 DATAOZ010000000
VERSION     DATAOZ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4419297"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VHR64_13545"
     CDS             complement(<1..626)
                     /locus_tag="VHR64_13545"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEX3303904.1"
                     /translation="MPSTFVLSRRALPVLIALLILLVGQPHLTGAHADLVTSNPAAGG
                     TISALPPKLTLTFAEEVKPDSPVVEVTGPDGQRVDAGDAAVDLTDATRKTVTVSLYAG
                     GPGAYAVHWETVSNTDGHPLSGDFTFTVSVAAASSPTAATALASPTAIATEDPTNGNP
                     LSPQSDFDSRALGLSVGAGLIAMAAIVGFWFAVRPRSPRFGPRAGRNR"
     gene            complement(779..1945)
                     /locus_tag="VHR64_13550"
     CDS             complement(779..1945)
                     /locus_tag="VHR64_13550"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX3303905.1"
                     /translation="MPRAAQLAGLIALLIALIGTPLAASAQQITSTSPPSNWSPPRTV
                     YITQTGQTIDGVFLDFWRANNGIANYGYPVTPEFTKDDGHVAQYYQYARYEYWPEAED
                     GQVVRLGDIGEDLRPKSLQRNSVASNGETDVKSGTSANAEVAQMTRAWLPLEKDSRKK
                     NSDTYVYVKESKHSISNGFKTWWDMTGGTSYLGNPLTQEYILKGTTYQVFERGQLAWE
                     KDKGVWLVPLGEVLAQQHQISTAPVAQGNVPAYSEELFVAPKQQKKGNGERWIEVNLT
                     TQYLIAWEGDVSIAETYVSTGRPGFDTPPGTYHILVKLESQTMEGVIGGEYYNVPDVP
                     WVQYFTNYGHALHGTYWHNNFGATMSHGCVNLPMDFAEWLYGWTSIGTRVEIHF"
     gene            complement(2195..3040)
                     /gene="nadC"
                     /locus_tag="VHR64_13555"
     CDS             complement(2195..3040)
                     /gene="nadC"
                     /locus_tag="VHR64_13555"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX3303906.1"
                     /translation="MQRQIDAIIDLALSEDIGPGDLTAESVIPDSARASGQMLLKSPG
                     VISGLSVVERVLRRVDPAIEWRPLVEDGCAFETRTVIGEISGPAQSVLTAERTALNFI
                     QRLSGVATLTRAFVDAIAGTSARIVDTRKTTPGMRVLEKQAVRHGGASNHRIGLYDGV
                     MIKDNHIAALGGDRAIGEAVARARNRIPHTVKIEVEVASLDQLRLALDSGADIILLDN
                     MPVEMMREAVAIAGGRAILEASGGVNLATVRAIAETGVDLISVGALTHSAAALDISLD
                     LRISG"
     gene            complement(3044..3496)
                     /locus_tag="VHR64_13560"
     CDS             complement(3044..3496)
                     /locus_tag="VHR64_13560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3303907.1"
                     /translation="MTDAPQVSPVAPEQTDLIIDKLNAWRDDMPAVDPAALTDKQPQV
                     FAVTLDGKVVGGYLMAQIPMANEIVLLAIDPAYRLRGLGRMCCMDALFRSGKRPLVLT
                     ANDASLGFARTVGFKLVGKRKQPDGSMLTRLGWHAPRPKSDPNAPPGC"
     gene            complement(3512..>3764)
                     /locus_tag="VHR64_13565"
     CDS             complement(3512..>3764)
                     /locus_tag="VHR64_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303908.1"
                     /translation="KELRKGRLLMGFEDSRSVASWIGSQELTYSEIKTPEEVMAKIDA
                     VTSGDVKELADELFQTNKLSLALIGPYSDEQVFRSIMQL"
BASE COUNT          652 a         1208 c         1203 g          701 t
ORIGIN      
        1 tcccggtttc gtcccgcgcg aggtccgaat cgcgggcttc ggggtcggac ggcgaaccag
       61 aagccgacga tcgccgccat cgcgatcaga ccagccccaa cactgagtcc gagcgcgcgg
      121 ctatcgaagt cgctctgcgg actgagcggg ttcccattgg tcggatcctc ggttgcgatc
      181 gcagtcggac tcgccagcgc cgttgccgcg gtcggcgacg atgcggctgc cacgctgacg
      241 gtgaaggtga aatcgccgga aagcgggtgg ccatcagtgt tcgagaccgt ctcccagtgg
      301 actgcgtagg cgcccgggcc gccggcatag agcgagacgg tcacggtctt gcgggttgcg
      361 tcggtgagat cgaccgcggc atcaccggca tcgactcgct ggccatccgg accggtcacc
      421 tcgacaaccg gcgaatccgg cttgacctct tccgcgaacg tcagtgtcaa cttgggcggc
      481 aaggctgaga ttgtcccgcc ggccgctggg ttcgacgtca caaggtcggc gtgcgcgccg
      541 gtgaggtgcg gctgcccaac cagcaggatc aggagggcga tcaggaccgg cagcgcgcgg
      601 cgggacagta cgaacgtgga gggcatgaag gtcagcatcc tggcctaaca cgaaatcatt
      661 cccctaagga taccgatgaa cgcgcgtcgc ggattgcgca tatcgggcac ccgtgcaagc
      721 gaaacccctg cccatcggac gatgtgacag gggtttcgag cgtcgtctgg agatgcgact
      781 agaagtggat ctcgacgcgg gtgccgatcg aagtccaccc gtagagccat tccgcgaagt
      841 ccatgggcag gttgacgcag ccatggctca tcgtcgcgcc gaagttgttg tgccagtagg
      901 taccgtgcag ggcgtggccg tagttcgtga agtactggac ccacggtaca tccggcacgt
      961 tgtagtactc gccgccgatc acgccttcca tcgtctggct ctcgagcttg acgagtatgt
     1021 ggtaggtgcc gggcggcgta tcgaagccgg gccgacccgt gctgacatag gtctcggcga
     1081 tcgaaacgtc gccctcccag gcgatcaggt actgggtcgt caggttgacc tcgatccagc
     1141 gctcgccgtt gcctttcttc tgctgcttgg gcgcgacgaa caactcctcg gaataggccg
     1201 ggacattgcc ctgggcgacg ggggcggtgc tgatctggtg ctgctgggcg aggacctcgc
     1261 cgagcggcac aagccagacg cccttgtcct tctcccaggc cagctggcca cgctcaaaaa
     1321 cctgataggt ggtgcccttg aggatgtact cctgcgtcag cgggttgccc aggtaagacg
     1381 tgccgccggt catgtcccac catgtcttga agccgttaga gatgctgtgc ttcgactcct
     1441 tgacgtagac ataggtgtcg ctgttcttct tgcgggaatc cttctccagc ggcagccagg
     1501 cgcgggtcat ctgcgcgacc tcggcgttgg cgcttgttcc ggacttgacg tccgtctcgc
     1561 cgttcgaggc gaccgagttg cgctggaggc tcttcggccg caggtcctca ccgatgtcgc
     1621 cgagacgaac aacttgcccg tcctcggcct ccggccagta ttcgtaccgc gcgtactggt
     1681 agtactgcgc gacgtggccg tcatccttgg tgaactcagg agtcaccgga tagccgtagt
     1741 tggcaatgcc gttattggcg cgccagaaat cgaggaagac tccgtcgatc gtctgcccgg
     1801 tctgcgtgat gtagaccgtg cgcggcggcg accaattgga cggcggagat gtactggtga
     1861 tctgctgcgc gctggccgca agtggggtgc cgataagcgc tatcagaagt gcgataagac
     1921 cggcgagttg cgccgccctg ggcatacgga aaaccggacg ccgacccgaa gacacgacgt
     1981 tcatgcgctt gtgctacccc tcataccgac ttgcgaaccg ccccagaaca acgtcaccgg
     2041 cagcgcaaat ccgccggtgc ctttgtcctt actcatgtga acgcacaaac ctgccgaaat
     2101 gtttcacgca ggtttacgca atattgtgcg ggattttccc agtttgtact acgtcaccca
     2161 ccgcaagtgt ggatttatgc cccaaccaat gtccctatcc cgagatccgc agatcaaggc
     2221 tgatatcgag cgcggctgct gaatgtgtca gtgcgccaac cgagatcagg tcgaccccgg
     2281 tctcggcaat cgcccgcacc gtcgccagat tgacgccgcc cgacgcctcg aggatcgccc
     2341 ggccaccggc gatcgccacc gcctcgcgca tcatctcgac cggcatgtta tcgagcagga
     2401 tgatatcggc gccggaatcg agcgccagcc ggagctgatc gaggctcgcg acctcgacct
     2461 cgatcttgac cgtgtgcgga atacggttgc gggcccgggc gaccgcctcg ccgatggcac
     2521 ggtccccgcc gagcgcggcg atgtggttgt ccttgatcat gacgccgtcg tagaggccga
     2581 tccggtgatt tgaggcgcca ccgtgccgga ccgcctgctt ctcgagcacg cgcataccag
     2641 gcgtcgtctt gcgcgtgtcg acgatccggg cactcgtgcc ggcgatcgcg tcgacgaagg
     2701 cccgggtcag ggtggcgacg ccggagagtc gttggatgaa gttcagcgcc gttcgctcgg
     2761 ccgtcaggac cgattgcgcc ggtccgctga tctcaccgat cacggtccgg gtctcaaagg
     2821 cgcagccgtc ctcgaccaac ggccgccatt cgattgccgg atcgactcgc cgcaaaacgc
     2881 gctcgacaac ggacaagccg gaaatcaccc cgggcgactt cagcagcatc tgtccggatg
     2941 cccgtgccga atcgggaatc acactctcgg cggtcaggtc gccgggtccg atgtcctcgc
     3001 tcagggcgag atcgatgatc gcgtcaatct gtcgctgcat ggctcaacac cccggaggcg
     3061 cgttcgggtc gctcttcggg cgcggcgcat gccagccgag gcgggtcaac attgatccat
     3121 ctggctgctt gcgtttgccg accagcttga acccgacggt ccgcgcgaag ccgagcgacg
     3181 cgtcgttggc ggtcagcacc agcggccgct tgccggaccg gaagagtgca tccatgcagc
     3241 acatccggcc gagcccgcgc agccggtagg ccgggtcgat cgcgagcagg acgatctcgt
     3301 tcgccatcgg aatctgggcc atcaggtacc cgccgacgac cttgccatcg agcgtgaccg
     3361 cgaagacctg cggctgcttg tccgtaagcg cggccggatc gaccgccggc atgtcgtccc
     3421 gccaggcatt gagcttgtcg atgatcagat cggtctgctc cggcgcaacc gggctgacct
     3481 gaggggcatc ggtcacggtc attcccgggc cctagagctg catgatcgag cggaatacct
     3541 gctcgtccga atacgggccg atcaaggcga ggctcagctt gttcgtctgg aacagctcgt
     3601 cggccagctc cttgacgtca ccggaggtga ccgcgtcgat cttcgccatc acctcttcag
     3661 gtgtcttgat ctcgctgtag gtcagctcct ggctgccgat ccacgaggcg actgagcggc
     3721 tgtcctcgaa tcccatcagc agccggccct tgcgcagctc cttg
//
LOCUS       DATAOZ010000532         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5736811, whole genome shotgun sequence.
ACCESSION   DATAOZ010000532 DATAOZ010000000
VERSION     DATAOZ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5736811"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VHR64_13570"
     CDS             <1..507
                     /locus_tag="VHR64_13570"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX3303909.1"
                     /translation="RRRDLMASGGSGVLLGLALLGFTAVYREGFEIVLFLQTLRLQAG
                     SGVVLQGVAIGLGFTAAVAILTFMANRKLPYKRMLVLTGVMLGFVLVVMVGESVQEMQ
                     LAGWVSTTPVDIPVPDWMGIWFAFFPTFETIVGQALAALLVIGSYVVARETRLIRIPG
                     RRDSGATA"
     gene            complement(497..2569)
                     /locus_tag="VHR64_13575"
     CDS             complement(497..2569)
                     /locus_tag="VHR64_13575"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303910.1"
                     /translation="MPPISPKPVTDYGPDYLPAYVSNGLIGLRVREIPLMAGVATVSG
                     LSGIDPTTQIMSSPYVPYPLAGDIRLGAVQMSDSPSNVRFIGQTYDFTCGELTTRFEF
                     TMEGVVARVEVLTFCSRTLPSLALQEVIVEVSKPVDVTLTALVDQRNVAGHWEHRWTS
                     VPGQETDLVDGTLAWTTFGEVATAGISYATEFSGDSNATCTKIAEEQAPLGTHYAFRA
                     RSNRRYRLRQFAGIVPNVLHHQPEAEAIRLVAEGRLLGFDELRRENREAWADIWRSRI
                     LIHGADERWQGLADAGYFYLMSSVHPSSPSSVHIFGLARWPNYHYYYGHVMWDVETFV
                     LPTLLLLQPDAARAILEYRTRSLDSARRNARLRGQMGVLFPWESDPERGEEATIGTAK
                     GASTEEHVNMAVALGFARYAHVTGDDWFLREQAWPVLRGVAEWIASRVERSDRGYAIR
                     RTKGIAERTQVENNNAYVNMAASMALREAVSAARRLGLDAPDHWQDIAECLVIPTDEK
                     RGVVLDHDDFDSAEEKGATPGPLAGFFPIGYPVDERVQRATTDFYLSQSDEYIGSPML
                     SALYGAWAARVGDRDAALRLFEEGYAAFVSERFMNTHEYREDRFPEQPIAGPFFANIG
                     GFLMSLLYGLTGIRISPDAPDTWPERPVCLPTGWQAIEVERLWARGRPMRLVARHGDS
                     HASLTPTQ"
     gene            complement(2796..>3319)
                     /locus_tag="VHR64_13580"
     CDS             complement(2796..>3319)
                     /locus_tag="VHR64_13580"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEX3303911.1"
                     /translation="TNPLPFMTDYTIIDGITGHGQYVGTYMAWGVNNAGWWGEGEIKF
                     YLDGDDQWPTICGTGTEDYFGGAWNFEHPKGEYGAFSTPFLGLPQIIMPDGLYRSQQR
                     FGMYRWHIMDPIRFQSDLRVTIQALGWRSSLEEQRRYLPLQDDIASTVFWYQAEPHAP
                     FPDAPSLNDLEVV"
BASE COUNT          607 a         1027 c         1056 g          629 t
ORIGIN      
        1 cgtcgccgtg atttgatggc ttcgggtgga tcgggcgtgt tgcttggcct cgcgctgctc
       61 ggcttcactg ccgtctatcg agagggcttc gagattgtcc ttttcctgca gacgctgcgg
      121 ttgcaggccg gttcgggtgt cgtgctgcag ggtgtcgcga tcggcctcgg cttcaccgcg
      181 gcggtcgcga tcctgacctt catggccaac cggaagctgc cgtacaagcg gatgctggtc
      241 ctgacaggcg tcatgctcgg attcgtgctg gtcgtgatgg tcggcgagag tgtccaggag
      301 atgcagctcg ccggctgggt ctcgacgaca ccggtcgaca tcccggttcc ggactggatg
      361 ggcatctggt tcgccttctt tccgaccttc gagacgatcg ttggccaggc gctcgcggca
      421 ctgctcgtga tcggctcgta cgtcgtggcc cgggagacgc ggctgattcg catcccgggt
      481 cgccgggact ccggcgctac tgcgtagggg tcaggctggc gtgactgtcg ccatgacggg
      541 cgacgagacg catcggccgg ccgcgcgccc agaggcgctc gacttcgatg gcctgccagc
      601 cagtcggcaa gcagaccggc cgctcgggcc aggtgtcggg tgcatccggc gagattcgaa
      661 taccggttaa tccgtagagc agtgacatca gaaagccgcc gatgttggcg aagaacggcc
      721 cggcgatcgg ctgttccggg aaacggtcct cacgatactc atgcgtgttc atgaatcgct
      781 cagagacgaa cgcggcatag ccctcctcga acaaccgaag cgccgcgtcc cgatcgccga
      841 cacgcgcggc ccacgcaccg tacagggcgg acagcatcgg gctgccaata tattcgtcgg
      901 attggctcaa gtagaaatcg gttgtcgcac gctggacgcg ctcatcgacc ggatagccaa
      961 tcgggaagaa gccagcgaga ggtccgggag tggcgccctt ctcctcggcc gagtcgaagt
     1021 catcatggtc aagtaccacg ccgcgcttct cgtcggtggg aatcaccagg cattccgcaa
     1081 tgtcttgcca gtgatccggc gcatccaggc cgagtctccg cgccgcgctg accgcctcgc
     1141 ggagggccat gctcgcggcc atattgacgt aggcgttgtt gttctcgacc tgagtgcgtt
     1201 cggcgatgcc cttggtgcga cggattgcgt acccgcgatc ggaacgttcg acgcgagacg
     1261 cgatccactc cgcgacgccg cgcagcaccg gccatgcctg ctctcgcaag aaccagtcgt
     1321 ctccggttac gtgggcatag cgggcgaagc cgagcgcgac cgccatattg acatgctctt
     1381 cggtcgaggc gcccttggcc gttccgatgg ttgcctcttc accacgctct ggatcacttt
     1441 cccatggaaa caagacgccc atctggccgc gcaatcgggc attccgccga gctgaatcca
     1501 gcgatcgggt tcggtattca aggatcgcgc gtgccgcgtc gggctgaaga agaaggagcg
     1561 tcggcagcac gaacgtctcg acatcccaca tgacgtgacc gtagtagtag tgataattcg
     1621 gccaccgcgc gaggccgaag atatggacac tgctcggcga cgaggggtga accgagctca
     1681 tgaggtagaa gtaaccggcg tccgcaaggc cttgccatcg ctcgtcagcg ccgtggatca
     1741 ggatccggct gcgccagata tcagcccacg cctcgcggtt ctcgcgccga agctcgtcaa
     1801 atcccagaag gcggccttcg gccaccagcc gaatcgcttc ggcttcaggc tgatggtgca
     1861 acacgttcgg cacgatgccg gcgaattgtc gcagtcgata ccggcggttg gagcgggcgc
     1921 gaaatgcata atgggtgccg agcggcgcct gctcttcggc gattttggtg catgtcgcgt
     1981 tcgagtcgcc cgaaaactcg gttgcgtaag agatgccagc ggtcgcaacc tcgccgaacg
     2041 tcgtccaggc caatgtgcca tcaaccagat ccgtttcctg cccaggcact gaggtccagc
     2101 ggtgctccca atgtccggcc acgttccgct ggtcaacgag cgcagtcaac gtgacgtcga
     2161 ccggcttcga cacctcgacg ataacttcct ggagtgccag tgacggcagc gtgcgactgc
     2221 agaacgtgag cacctcgacg cgcgcaacaa ccccttccat ggtgaactcg aatcgcgtcg
     2281 tcaactcccc gcatgtgaag tcatacgttt gcccgatgaa tcgaacattc gagggcgagt
     2341 cgctcatttg caccgctccc agtcgaatgt ctcccgcgag cggatatggc acatatggcg
     2401 agctcatgat ctgcgtagtt ggatcgattc ccgagagtcc actgactgtc gcgacgcccg
     2461 ccatcagcgg gatctccctg acgcgtaacc cgatcaagcc attggaaaca tacgccggca
     2521 agtagtcggg gccgtagtca gtaacaggct tcgggctgat cggaggcatc gcttacacct
     2581 caacttcgca tggttcgctc gtaggcatat cctgattaca ccgccagtct agtcaggcgc
     2641 acgagctcat ccgggggatg ctcttcggta gagatggaat atccaggagt gggcaatgtc
     2701 gacatccctg aggcacgcga aatgacctac ccgccgaagg atagggaggc tgggaccggc
     2761 caatcgtcgg cctactacag tgccggtagc gcgcgctaaa caacctcgag gtcgttgagc
     2821 gacggggcgt ccgggaaggg cgcgtgcggc tcggcctggt accagaagac ggtcgaggcg
     2881 atgtcgtcct gcaagggcaa ataacgccgc tgctcctcta gcgatgagcg ccagccgagc
     2941 gcctggatcg tcacgcgcag atcgctctgg aatcggatcg ggtccatgat gtgccagcgg
     3001 tacatgccga agcgttgctg gctgcggtag agaccgtccg gcatgataat ctgcggcagg
     3061 ccgaggaagg gtgtcgagaa cgcgccgtac tcgcccttcg gatgctcgaa gttccacgcc
     3121 ccgccgaagt aatcctccgt gccggtccca cagatggtcg gccattggtc atcgccgtcg
     3181 aggtagaact tgatctcgcc ctccccccac cagccggcat tgttgacgcc ccaggccatg
     3241 taggtgccga cgtactggcc gtggcccgtg atgccgtcga tgatggtgta gtcggtcatg
     3301 aacggcagcg ggttggtgc
//
LOCUS       DATAOZ010000533         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8105647, whole genome shotgun sequence.
ACCESSION   DATAOZ010000533 DATAOZ010000000
VERSION     DATAOZ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8105647"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VHR64_13585"
     CDS             complement(<1..648)
                     /locus_tag="VHR64_13585"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEX3303912.1"
                     /translation="MVGFDGLGMGLGNLARLSRAETRSISAENFTGEKGRGGMATDGT
                     GAVPARELGQGWKVSPSINVAGNATVVLADIAGPGAIQHIWLTVHPTHWRRIMFRFLW
                     DDEPTPSVEVPIGDFFANGWCERCNISSLPIAVNPAGGFNSYWEMPFRTSARIEIENL
                     GPDEIRGFYYQIDYTLTDIPEDRAYFHAQWRRSNPLPYQTDHTLLDGVRGQGHYVG"
     gene            930..2249
                     /locus_tag="VHR64_13590"
     CDS             930..2249
                     /locus_tag="VHR64_13590"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PotD/PotF family extracellular solute-binding
                     protein"
                     /protein_id="HEX3303913.1"
                     /translation="MADRDQTVEQLYQEAVSKRLNRRAILARGMALGLSLPAIMSVAA
                     QAQDASPAASPAPMTSPAQNGPVHVSIVDKDMSFDDIKAAISDEKEVTVANWTYTAND
                     QLVKRFQDYVKTVYDVDVKLNYIGSQSPSTYIADLYTTAAAGGDSAYDVMAIEENYWA
                     QVQSDSKAQNTTFMQDYLPSGLIPNAERVMDSLKHYPTTVGFQASATPGINYNKGTVD
                     FLKDWKDLADERLKGKILLWLPGDITGGGMLLGMCDSLGLDYHNPDHVTQAIDFIIDK
                     VGPNVLKYTSDNAEAQSLFKSGAVDVVSFWNSMARLQFLDGMEDAAFLVAASGQYAVN
                     GYMWIPVKAKHPVLAQIFIDWRLSDDAQFPDLDAWGITKGAWAELQEGFMGPSYEGDV
                     PAWIKDVYFTYYPTIDQLSTSYKAVDWDYYVAHSSDWFDYWNQKLGL"
     gene            2262..3188
                     /locus_tag="VHR64_13595"
     CDS             2262..3188
                     /locus_tag="VHR64_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3303914.1"
                     /translation="MARAIAPRPCIMSATTATDAIRPAIAQNRRKVWLGRLTGPGLVL
                     PAAVVTVLFSLVPIAYLVLVSLTHKSDFFFGKRDYSLANYDLVFNRYWQNVVVTIRLG
                     LLASILNLVWGYPFAYILIRKVRYREVVRTFMAFPLFGPLYLAFGLYYILLPGGPLAG
                     LLDFLHIKGTDLLYSEPMTLFGMALFTFPFMVMNVGAALSNVDPMLEEAAKTLGAKPW
                     QVFLRVLFPLSRSGILAGFLMCFGWNIGVFIVPILLGGLAQQRVLSLILQQKAMAQFD
                     YGLAAAMGMVLMFIAFAVTWLALKFSRGALGA"
     gene            3190..>3894
                     /locus_tag="VHR64_13600"
     CDS             3190..>3894
                     /locus_tag="VHR64_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3303915.1"
                     /translation="MSAIAAQPTRREGFRFRRRWIGDILAHGYIWFWLALFALPFVAT
                     TIHSLELPDGGYGFAAYRFAWGSFKYDLLTSFKVTGLTILINLLLSVPAAYAFVRYPF
                     PGKRALLSILNLSLYTPAAVMGLSLLITYAFLFHIPRSVTGLVAAYVVGSYPLMLIPI
                     IVALRDLPMVYEEAAKTLGANRLQSLFRIELPLLGPGISAGVLLTFVIIFNEFLVTLF
                     IAGPNTQTAALRVYGLT"
BASE COUNT          730 a         1193 c         1149 g          822 t
ORIGIN      
        1 gccgacgtaa tgaccctgac cgcggacgcc gtcgagcagc gtgtggtcgg tctgataggg
       61 caggggattg ctccgccgcc actgggcgtg aaagtaggcg cgatcctcgg ggatgtcggt
      121 tagcgtgtag tcgatctggt agtagaaacc gcggatctca tcaggcccga ggttctcgat
      181 ctcgatccgg gctgatgtgc ggaatggcat ctcccagtaa ctgttgaagc cgccggcggg
      241 gttgaccgcg atgggtagcg agctgatgtt gcagcgctcg caccagccgt tggcgaagaa
      301 gtcgccgatc ggaacctcga ccgacggcgt cggctcgtcg tcccacaaga agcggaacat
      361 gatccggcgc cagtgcgtgg ggtggacggt cagccagatg tgttggatcg cgccgggtcc
      421 ggcgatgtcg gccagcacga ccgtcgcatt gccggcgaca ttgatgctcg gcgagacctt
      481 ccagccctgg ccaagctcgc gggccggcac ggcgccggtc ccgtcggtcg ccatcccgcc
      541 gcgacccttc tcgccggtga agttctccgc gctgatcgac cgggtctctg cccgcgacaa
      601 tcgagccaga ttccccagtc ccattcccaa accgtcaaat ccaaccaatg gaagctccct
      661 tcaacgtaga cgggattatg agtcgagaag tcgaaaagtc gggaagtcgg gaagtcgagc
      721 aggcggaaaa tgaaagacga aaggcgaaag accaaaaacc aaagaccaaa cttctgctag
      781 aaaccagaaa cgagaaactg tccactgtcc aatgaccaac gttcccgtgc gctcttgacc
      841 aaatgggcta tgcctcatac tgttccgtac tgttcagcgc tgtcggtttg gcaacatgcg
      901 ggccttcggc ctgcgggagg atttgcaccg tggcagatcg tgatcagaca gttgagcagc
      961 tctaccagga agcagtcagc aaacgtttga atcgccgggc gatcctcgcc cgcggcatgg
     1021 cactggggct ctcgcttccg gcgatcatgt cggtcgccgc ccaggcacag gacgcgtccc
     1081 cggccgccag tcctgcacca atgacttctc cggcccagaa cggaccggtc catgtctcga
     1141 tcgttgacaa ggacatgtcc ttcgacgata tcaaggccgc gatttccgac gaaaaggaag
     1201 tcaccgttgc caactggacc tatacggcca acgatcaact ggtcaagcgg ttccaggact
     1261 acgtgaagac cgtctacgac gtcgatgtca agctcaacta catcggctcg cagtcgccca
     1321 gcacgtatat cgcagacctc tacacaacgg ccgcggcggg tggtgactcc gcatacgacg
     1381 tgatggcaat cgaggaaaat tactgggcgc aggttcagtc cgattccaag gcccagaaca
     1441 cgacgttcat gcaggattac ctgccgtccg gcctgatccc gaacgccgag cgggtcatgg
     1501 acagcctcaa gcactacccg acaacggtcg gcttccaggc ctctgcgacc cctggcatca
     1561 attacaacaa gggcacggtt gatttcctga aggactggaa ggacctcgct gacgagcggc
     1621 tcaagggcaa gatcctgctc tggctgccgg gcgacattac cggcggcggc atgctgctcg
     1681 gcatgtgcga ttcgcttggg cttgattacc acaaccccga ccacgtcacc caggcgatcg
     1741 atttcatcat cgacaaggtc ggtccaaacg tcctcaaata cacgtcggac aacgcggaag
     1801 cgcagtcgct cttcaagagc ggcgccgtgg atgtggtgtc gttctggaac tcgatggccc
     1861 gcctgcagtt cctcgacggg atggaagacg cggccttcct ggtggcggcc tccgggcagt
     1921 acgccgtcaa tggctacatg tggatcccgg tcaaggcgaa gcatccggtg cttgcccaga
     1981 tcttcatcga ctggcgcctc agcgatgatg cccagttccc ggatctcgat gcctggggca
     2041 tcaccaaggg cgcgtgggcc gagctccagg aaggcttcat gggcccgtcc tacgagggcg
     2101 atgtccctgc ctggatcaag gacgtctact tcacctacta cccgacgatc gatcagcttt
     2161 cgacaagcta taaggcggtc gattgggact actacgttgc ccactcgtcc gattggttcg
     2221 actactggaa ccagaaactc ggtctatagg agcaaatcgc cgtggcgcga gcaatcgcgc
     2281 cacggccctg cattatgagc gcgaccacag caaccgacgc gattcgaccg gcaattgccc
     2341 agaaccggag aaaggtttgg cttggtcgcc tgaccggacc cgggctggtg cttcctgccg
     2401 cggtggtgac cgttctgttc tcgctggtgc ctatcgcgta tcttgtgctc gtcagcctga
     2461 cgcacaagtc cgatttcttt tttggcaagc gcgattacag cctggccaac tacgacctgg
     2521 tgttcaaccg ctattggcag aatgtggtgg tcaccatccg gttgggcttg ctggcctcga
     2581 ttctcaacct cgtctgggga tacccgtttg cctacatcct gatccggaag gtccgctatc
     2641 gcgaagtcgt tcgaacgttc atggcgtttc ctctattcgg cccgctctac ctcgcattcg
     2701 ggctctacta cattctgctg ccgggtggcc cgctggctgg actgctcgat ttcctgcata
     2761 tcaaggggac ggacctcctt tattcggagc cgatgacgct gttcggcatg gcactgttca
     2821 cgttcccgtt catggttatg aacgtgggcg cagccctctc gaacgttgat cccatgctcg
     2881 aagaggcggc caaaaccctc ggcgccaagc cgtggcaggt gttcttgcgg gtgctgtttc
     2941 cgttgagccg cagcggcatt ctggccggtt tcctgatgtg cttcggctgg aatatcggcg
     3001 tcttcatcgt gccaatcctg ctcggcggtt tggcgcaaca gcgcgtcttg tcattgatcc
     3061 tccagcagaa ggcgatggcc caatttgatt acggactggc ggcggcgatg ggaatggtcc
     3121 tgatgttcat cgccttcgcc gtcacctggt tggcgctcaa gttctcgcgc ggcgcattgg
     3181 gagcctgaga tgagcgcgat cgccgcccaa ccgactcgac gcgaaggctt ccggttccgc
     3241 cgccggtgga tcggcgacat cctcgcgcac gggtacatct ggttttggct ggcgctgttc
     3301 gcgctgccat tcgtggcgac gacgattcat tcgcttgagc tgccggatgg cgggtacggg
     3361 ttcgccgcat accggtttgc ctggggttcg ttcaaatacg acctcctgac ttcgttcaag
     3421 gtcacgggcc tgacgatcct gatcaacctg ctgctctcgg tgcccgcagc ttatgcgttc
     3481 gtgcgctatc cgtttcccgg caagcgcgcg ttactctcga tcctgaactt atcgttgtac
     3541 actccggccg ccgtgatggg gctgagcctg ctgatcacat acgcattcct ctttcacatc
     3601 ccgcgttcgg ttaccggttt ggtcgcggcg tacgtcgtgg gcagctatcc actcatgctc
     3661 attccgatca tcgtggcgct gcgtgaccta ccgatggtct acgaggaggc ggcgaaaacg
     3721 ctcggcgcca atcgtctcca gagtcttttc cgaatcgaac tacctctgct gggacccgga
     3781 atcagcgccg gggtactcct gacgttcgta atcatcttca atgagttcct ggtcacgctg
     3841 ttcattgcgg gtcccaacac gcagaccgcg gcgctccgtg tctatgggct gacg
//
LOCUS       DATAOZ010000534         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_737608, whole genome shotgun sequence.
ACCESSION   DATAOZ010000534 DATAOZ010000000
VERSION     DATAOZ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737608"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="VHR64_13605"
     CDS             <1..1427
                     /locus_tag="VHR64_13605"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3303916.1"
                     /translation="LEQGLLGAAFPPDFKTSGLFYVYYADYTTNGNLFLVQYHVSADD
                     PNKADPDSAKLIYATPKYPNPGDPYVNHNGGTVRFGPDGYLYWTTGDGGLAGDPYDNA
                     QNIRNEFGKIHRIDVSGSSGTEPYKIPDDNPFAASSAVNPAGFAIEDPANYHPGADPT
                     IWAYGLRNPWQFNFDPKTGDLYIADVGQNAWEEIDFQAAGSPGGQNYGWDWLEGSHCY
                     PEYVSDCPRSQIGVLPVAEYDHTQGDCSISGGGVSRTSESPNIDGVYFASDFCSGRVW
                     GLKQDDSGTWQFQQLLDTQLLVTGGGSGEDGAVYFTSCNCVFDRTYDPFANPQGAVWR
                     VVESDKVPSGAVTPPPEGAATPTAGTTGNEATPAAATPATGAATPAAASSGGNAQTVE
                     MVDIAFNPKELTIPANTDVTLSLPNNGVSLHNFSIDELNIDVDVAPGETGSATINAPA
                     GTYQYYCNVPGHKEAGMVGTLTVQ"
BASE COUNT          299 a          524 c          467 g          256 t
ORIGIN      
        1 tcctggagca aggactgctc ggggctgcat tcccgcccga tttcaagacc agcgggctct
       61 tttacgtcta ctacgcggac tacaccacca acggcaacct cttcttggtg caataccacg
      121 tttcggccga cgacccgaat aaggccgatc ccgacagcgc caagctgatc tatgccacgc
      181 ccaaataccc gaaccccggc gatccgtatg tcaaccacaa cggcggcacg gtccgcttcg
      241 gacccgatgg ctacctctac tggacgaccg gtgacggcgg actcgcgggc gatccgtatg
      301 acaacgctca gaacatccgc aacgagtttg gcaagatcca ccgcatcgac gtcagcggct
      361 catccggcac cgagccctac aagatcccgg acgacaatcc gttcgcagcg tcgtctgcgg
      421 tcaacccagc cggtttcgcg atcgaagacc cggcgaacta ccatcccggc gccgatccga
      481 cgatctgggc gtatggtctg cgcaacccct ggcagttcaa cttcgatccg aagaccggcg
      541 atctctacat cgccgatgtc ggccagaacg cctgggagga gatcgatttc caggccgcgg
      601 gctccccggg cggtcagaac tacggctggg actggctcga agggtcgcat tgctaccccg
      661 agtacgtgag cgattgcccg cgctcgcaga ttggcgtgct gccggtcgcg gaatatgacc
      721 acacccaggg cgactgctcg atctccggcg gcggcgtctc gcgcaccagc gaatcgccga
      781 atatcgacgg cgtctacttc gcctccgact tctgctccgg ccgcgtttgg ggcctgaagc
      841 aggacgacag cggaacctgg cagttccagc aattgctcga cacccaactc ctcgtcaccg
      901 gtggcggtag cggcgaggat ggggccgtct acttcacctc ctgcaattgt gtcttcgacc
      961 gcacctacga tccgtttgcg aacccgcagg gcgcggtgtg gcgtgtcgtg gaaagcgata
     1021 aggtgccctc gggcgcggta acgcctccgc ccgaaggcgc ggcgaccccg accgctggga
     1081 caactggaaa tgaggcgacc ccggccgcgg ctacaccggc gacaggcgcc gcgacgccgg
     1141 ccgcagcgtc gagtggcggc aacgcgcaga cggtcgagat ggtcgacatc gcgttcaatc
     1201 cgaaggaatt gacgattccg gcgaacaccg atgtcacgct ctcgctgccg aacaacggcg
     1261 tgtcgttgca caacttcagc attgacgagc tgaacatcga tgtcgatgtc gcgccgggag
     1321 aaaccggttc ggcgacgatt aacgcgccgg ccgggaccta tcagtattac tgcaatgtcc
     1381 ccggccataa ggaagccggg atggtcggca cattgacggt gcagtaaaga cgaccctcac
     1441 ccgctgctgc ccagctgccc tctcccagcg tcgctgggag agggcacggc ggctcgcgat
     1501 catgcggcgc cctcccgccc cgtcatcctg agtggaggcg gagccg
//
LOCUS       DATAOZ010000535         4978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3108208, whole genome shotgun sequence.
ACCESSION   DATAOZ010000535 DATAOZ010000000
VERSION     DATAOZ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3108208"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VHR64_13610"
     CDS             complement(<1..745)
                     /locus_tag="VHR64_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3303917.1"
                     /translation="MNSAAPAGIDTSLETGIILVVEDDPSLCSALCYNLRRAGHTAIA
                     AHDGESALRTFTEQRREIDLVLLDIMLPRLSGLQVLRVIRESSTVPAVILSARGEEQD
                     RINGLDLGADDYLVKPFPMREMMARVRAQLRRRPNREAGIPSVIEHGPLRIDTGSRRA
                     WVNARELALRPKEYGLLLALANDAGKLFTRQDLLDAVWGADIFVDERTVDVHVSWLRG
                     KLRRVGMTRDLIQTVYGAGYRLNAETGDHL"
     gene            931..1257
                     /locus_tag="VHR64_13615"
     CDS             931..1257
                     /locus_tag="VHR64_13615"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX3303918.1"
                     /translation="MPLYLSRFSYTPESWARLIANPEDRRTAAQQYIESVGGKLHGFW
                     YAFGSHDGYNLWEAPDNVSMAAVALAIAGGGALSSFETTVLLTVDETLDALRRAGQVR
                     YHPPGA"
     gene            complement(1291..1734)
                     /locus_tag="VHR64_13620"
     CDS             complement(1291..1734)
                     /locus_tag="VHR64_13620"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX3303919.1"
                     /translation="MTSTEQQNMELMQTLDDAWNAQDAETFRKRHKDDVVVTWPGGNP
                     PTRGIEDHTAESVAFWKTFPDQKLDNRPYRVFFASGDWTCSIARFRGTMTGPMMGPDG
                     KEIPPTGKSFEVDFCTVAHWGNGQITEENLFYDLVGFMKQIGLSG"
     gene            complement(1956..3074)
                     /locus_tag="VHR64_13625"
     CDS             complement(1956..3074)
                     /locus_tag="VHR64_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HEX3303920.1"
                     /translation="MAASELSRYQANLQDEIDGVALYRALAEVETDPALAGIYGRLAD
                     AEERHAELWRGKIRDAGGTLGPEQPAWRTRVLIGLARRFGPALVLPTISGREEADRNK
                     YAGQPDAKAAGLSSEEQSHARLFQAIAPEGSGGASGGMIAQFEGRHRASGGNALRAAV
                     LGANDGLVSNASLVMGVAGADLAGRNILITGLAGLLAGSLSMALGEWLSVQSARELYS
                     HQIAIERDELEAAPEEEAEELALIYQSKGLAPERAQELANSIIRDQTAALDTLAREEL
                     GVDPSELGGSAWVAAITSFVLFSIGAIVPVFPYFFGGGTSAVIVSVILSVIALFVIGA
                     AITVITGRSVLMSGGRQVVFGMAAAAITFGVGRLIGGV"
     gene            3339..4754
                     /locus_tag="VHR64_13630"
     CDS             3339..4754
                     /locus_tag="VHR64_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3303921.1"
                     /translation="MVVATRPSTSSFTVVSPETPLDELRSKLVGQLLTADSIEYEQAR
                     RTQNLKSNRFPKAIVRAASEYDVSLAVAFAREQGLPLAVRSGGHSLALYSVVDDALVI
                     DLSGMKRVRIDPDERIAYVQPGATSGDLAVPAGEHGLALSTGDARSVGMGGLTTGGGI
                     GFMVRKYGLAIDNLLAARVVLADGSIVTTSATERPDLFWAIRGGGGNFGIITEFTYRL
                     APVGQIYGGALILPATKEVIRGYLDYAKSAPEDLTTITNMMFAPPAPFIPEDKVGSLV
                     LIILPVWTGSMEDGEKVMAPLRALAEPVADLVGPMPYPAIYTFTDFATEPHGAALRSM
                     FSDDLSDDAIDAILDAMATASSPFALVQLRPLGGALSKVEKYATAFAHRDKDLMLAII
                     NIWLDPAEDSAPHAAWTEALWAKVRGEGEGAYVNFLEEEGEARIRDAYPPATYEKLAK
                     IKAYYDPENVFRFNQNIKPSA"
BASE COUNT          880 a         1655 c         1584 g          859 t
ORIGIN      
        1 gaaggtgatc tccggtctcc gcgttcaggc ggtacccggc gccgtagacc gtctggatca
       61 ggtcgcgagt catcccgacc cggcgcagct tgccgcgcag ccaggagaca tggacatcga
      121 ccgtccgctc gtccacgaag atatccgcgc cccagaccgc atcgagcagg tcttggcggg
      181 tgaagagctt gccggcgtcg tttgccagtg ccagaagcag gccgtactcc ttcgggcgca
      241 gcgcgagctc gcgcgcgtta acccaggcgc ggcgactgcc ggtatcgatc cgcagcgggc
      301 catgctcaat gaccgagggg atcccggcct cgcgattcgg ccgtcggcgc agctgcgcgc
      361 ggacgcgggc catcatctcc cgcatgggga agggcttgac caggtagtcg tcggcgccca
      421 gatccagccc gttaattcga tcctgctctt cgcccctcgc tgagagaatc acggccggga
      481 ccgtcgagct ctcccggata acccgcagca cctgcaggcc ggagaggcgg gggagcatga
      541 tgtcgaggag gaccagatcg atctctcggc gctgctcggt gaaggttcgc agcgcggact
      601 caccgtcgtg cgcggcgatc gcggtgtgac ctgcccggcg caggttatag caaagcgcgc
      661 tgcagagcga gggatcgtcc tcgacgacga gaatgatccc agtctcgagt gaggtatcga
      721 ttccggcagg cgcggcgctg ttcatgtctc cattgtgggg agaccgtgtt aatggacggt
      781 taccgcggag ttaacgttcg gcaaaattcg gccaccgatc gcggcaacgt gcacgttttc
      841 cggacatgca ataatcgaat tgccttggac aggtcatccg cggatatccc gcgtcacctc
      901 gcgtgggatg gcgaacgaag ggagagagcg atgccgctgt atctttccag gttcagctac
      961 acgccggaat catgggccag gttgatcgcc aatccggagg atcggcggac ggccgctcag
     1021 cagtacatcg agtcagtcgg cgggaagctc catgggttct ggtatgcctt cggctcgcac
     1081 gatggctaca acctctggga agcgcccgac aacgtctcga tggccgcggt cgccctggcg
     1141 atcgccggcg gaggcgcatt gagctccttc gagacgaccg ttctcctgac tgtggacgag
     1201 acgctggatg cgctgcgccg ggccggccag gttcgctatc acccacccgg cgcgtagccg
     1261 cgaattgtca gacgtacggg atttgccgcg ttagccggag aggccgatct gcttcatgaa
     1321 accgacgagg tcgtagaaga ggttctcctc cgtgatctgg ccatttcccc agtgcgcgac
     1381 ggtgcaaaaa tcgacctcga acgacttgcc cgtcggcggg atctctttgc catcgggccc
     1441 catcattggg ccagtcatcg tgccccggaa gcgggcgatt gagcaggtcc agtcgcctga
     1501 cgcgaagaag acgcggtagg gtcggttgtc gagcttctgg tccggaaacg tcttccagaa
     1561 ggcgacggac tccgctgtat ggtcctcgat gccgcgcgtc ggcggattac cgccgggcca
     1621 ggtgacgacg acatcgtcct tgtggcgctt gcggaacgtt tccgcgtcct gggcattcca
     1681 cgcatcgtcc agggtctgca tcaactccat gttctgctgc tcggtgctgg tcatggcgtt
     1741 gctccttgcg agattcggac gcaaacgatc ggacgcgctg cccgcagcga tggggcatct
     1801 tgcggtgatg gcggtacgga tggatgacag ttccggattt ccaccccttg tccattccgc
     1861 taccggagca gcgtagagca tccaccgacg cgcacgacgc actcgtttcg agccgaaggt
     1921 gatgaccgtt accggctgaa ccgccggtct gacgatcaca cgccgccgat gagtcgcccg
     1981 acgccgaagg tgattgccgc ggcggccatg ccgaagacga cttgccgacc tccggacatc
     2041 agcacgctgc ggccggtgat aacggtgatc gccgcgccga tgacgaagag ggcaatcacg
     2101 ctcaggatca cgctgacaat caccgcgctg gtcccgccgc cgaagaaata cgggaaaacc
     2161 ggaacgatcg cgccgatcga gaacagcacg aatgaggtga tcgccgcaac ccaggccgaa
     2221 ccgcccagct cggacggatc cacgcccaac tcctcacggg ccagggtgtc gagcgcggcg
     2281 gtctggtcgc ggatgatgct gttcgccagc tcctgtgctc gctcgggcgc cagccccttg
     2341 gactggtaga tcagcgccag ctcctcggcc tcctcctccg gcgcggcctc caactcgtcc
     2401 cgctcgattg cgatctggtg cgaatagagc tcgcgcgcgc tctggaccga gagccactcg
     2461 ccgagtgcca tcgagagcga cccggccagg agtccggcga gtccggtgat caggatgttc
     2521 cggccggcca ggtcggcgcc ggccacgccc atgacgagac tggcattcga gacgagcccg
     2581 tcgttggcgc caagcactgc tgcccgcagc gcgttgccgc ccgaggctcg atgcctcccc
     2641 tcgaactggg cgatcatgcc gccgctcgcg cctccgcttc cttccggcgc gatggcctgg
     2701 aagaggcggg cgtgggattg ctcctcggac gagagcccgg ccgccttcgc atcgggctgc
     2761 ccggcgtact tgttgcgatc ggcctcctcg cggccggaga tggtcggcaa cacaagagcc
     2821 ggaccgaagc gccgggccag tccgatcagg acgcgcgtcc gccaggccgg ttgctccggt
     2881 cccagcgtgc cgccggcatc ccggatcttg ccgcgccaga gctcggcgtg ccgctcctca
     2941 gcatcggcca gccggccata gatcccggcg agcgccgggt cggtctccac ctccgccagc
     3001 gcccggtaca gcgcaacgcc gtcgatctca tcctgcaaat tggcctggta tcgggaaagt
     3061 tcggatgcag ccataggtga ctccggaacg gaacgtgact gccaatcaca actgcgagtc
     3121 tacacctcgt caggcctgaa atcgcccgga ttaccgcgct gtttcggcag tcccgaaccg
     3181 tcggaggact gagcgagcca tccatcgaaa ggctgagcaa cggttcagcc gctcgccgca
     3241 tgtgcatgca tgagcgagcc gataaaacgg tttaggcaac gcccaaccgg gcaaaacaaa
     3301 ctggaattca ggcgctgaaa cgccaaaagg agtcactcgt ggttgttgca acaaggccct
     3361 cgacctcgtc cttcaccgtc gtatccccgg agactccgct cgatgagctg cgctcgaagc
     3421 tggtcgggca gctgctcacg gccgacagca tcgaatacga gcaggcgcga cggacccaga
     3481 atctcaagtc caaccgcttt ccgaaggcga tcgtccgcgc cgcgagcgag tacgacgtct
     3541 cgctggccgt cgcctttgcc cgcgagcagg gtctcccgct ggcggtgcgc agcggcggtc
     3601 acagcctggc cctctactcc gtggttgacg atgctctcgt gatcgatctc tcgggcatga
     3661 agcgtgtccg catcgatccg gacgagcgca tcgcatatgt ccagcctggc gcgacctccg
     3721 gcgacctggc cgttccggcc ggcgagcacg gtctcgccct ctcgaccggc gatgcccgct
     3781 cggtcggcat gggtggtctg accaccggtg gcggtatcgg cttcatggtc cgcaagtacg
     3841 gcctcgcaat cgacaatctg cttgccgcgc gagtcgtgct cgctgacggc tcgatcgtca
     3901 cgaccagcgc gactgagcgg ccagacctct tctgggcgat ccgcggtggt ggcggcaact
     3961 tcggcatcat caccgagttc acctatcgcc tggcgccggt cggccagatc tatggcggcg
     4021 cactgatcct cccggcgacg aaggaagtca tccgcggcta cctcgactac gcgaagtccg
     4081 ccccggagga tctgaccacg atcacgaaca tgatgttcgc accgccggcg ccgttcattc
     4141 ccgaggacaa ggtcggctcg ctggtgctga tcatcctgcc ggtctggacc gggagcatgg
     4201 aggacggcga gaaggtgatg gcgccgctgc gtgccctggc tgagccggtg gccgacctgg
     4261 tcgggccgat gccgtacccg gcgatctaca ccttcacgga ctttgcgact gagcctcacg
     4321 gcgccgcgct ccgctcgatg ttctccgacg acttgagcga cgacgcgatc gacgcgatcc
     4381 tggatgcgat ggcgaccgca tcatctccgt tcgcgctggt ccagctgcgc ccacttggcg
     4441 gcgcgctgtc aaaggtcgag aagtacgcga ccgccttcgc gcatcgcgac aaggacctca
     4501 tgctggccat catcaacatc tggctcgatc cggccgagga ctccgcgccg cacgccgcct
     4561 ggaccgaggc gctctgggcg aaggtccgcg gcgagggcga aggcgcctat gtgaacttcc
     4621 tcgaggagga gggcgaggcc cggatccgcg acgcgtatcc gccggccacg tacgagaagc
     4681 ttgcgaagat caaggcctat tatgacccgg agaacgtctt ccgcttcaac cagaacatca
     4741 aaccgagcgc gtaacgacaa gcgctcctct ccatacccct ctcacacccc tccccaggaa
     4801 gcgcgccgat cggcgcgctt ccttcattct ccgagcaccg acacccaacc tggtgcagat
     4861 gctaactacc caatgtggcg cggaggatta aatatggcgc ggggcctctg tgccccgcga
     4921 tcgctcaaaa agtggtgaag tggcaactgc tctgaaaaag gcgtgctcgg cgtcgccc
//
LOCUS       DATAOZ010000536         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2844471, whole genome shotgun sequence.
ACCESSION   DATAOZ010000536 DATAOZ010000000
VERSION     DATAOZ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2844471"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VHR64_13635"
     CDS             <1..841
                     /locus_tag="VHR64_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303922.1"
                     /translation="SPEAIAEGLLACVSPILRPRAGLAPTRFSAWVAARMASPGTVRT
                     IQPADLATTLGPLPVSWLPLEPNTLLKLDRLGLRTLAEIRELPASALQARFGPAGREA
                     HACAAGQDEGRIYPRKLVESVREEFRLPAPMASRDMLLLAIRQLVIRAFNRPALRHRQ
                     IRQSRIRIAIEGGQSWERELTFREPVGSQRLIETLRSRLQAIELPGPAETIHLDLIGI
                     VNEIATQELIPLLRPKRDRPLIEAARQLKQRYGESPLAHIVEVEPWSRIPERRFALIS
                     YDP"
     gene            790..1071
                     /locus_tag="VHR64_13640"
     CDS             790..1071
                     /locus_tag="VHR64_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303923.1"
                     /translation="MVAHSGTPIRPDQLRPLNRPRRCTVQTDRGVPIAIIEGKRHEPV
                     ERVQDRWQLNDEWWRSPIERHYFELVMADGRIRTVYHDRQRDEWFEQRG"
     gene            1068..>1952
                     /locus_tag="VHR64_13645"
     CDS             1068..>1952
                     /locus_tag="VHR64_13645"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX3303924.1"
                     /translation="MSAYTELHLHTSFSFLDGASQPEELIARALELGFDSLAVTDHNG
                     LYGAMEFAQLANGVGVRPITGAEVTLLDGTHLTLLAENQRGYVNLCRLLTEAHRLRDP
                     NPPSRRLEIPFPLRTVGVSRPAQRDETPDPRLDPTLLATHGEGIILLTGCRQGQISQL
                     LDDGQFALAEEVLRRYVDWLGPDQVFVELQHNLVFGDTRRVAHMAQLAEHVGVRYVAT
                     GNVHYHVPQRHRLNDVLVAIRSHSNLDGCHRERRPNDQFYLRSADEMAERFAAYPEAL
                     ATTRLISERCASFNLAKDL"
BASE COUNT          308 a          700 c          634 g          310 t
ORIGIN      
        1 gtcgcccgaa gcgatcgccg aggggttgct ggcctgcgtc tcgccgatcc tgcgaccgcg
       61 cgccggcctg gctccgactc gcttctcggc ctgggtcgcg gcccgaatgg cttcgccggg
      121 gacggtccgg acgatccagc cggctgatct cgcgacgacg ctcggtccgc tcccggtctc
      181 ctggctgccg ctcgagccaa atacgctcct gaagctggac cggctcggct tgcgcacgct
      241 ggccgagatc cgcgagctgc cggcctccgc tctgcaggcc cggttcggac cggccggtcg
      301 cgaagcccat gcctgtgcag ccggacagga tgagggccgg atctacccgc gcaagctggt
      361 cgagtcggtc cgcgaggagt tccgcttgcc ggcgccgatg gccagccgcg acatgctcct
      421 gctcgccatc cgccagctcg tgatccgcgc cttcaaccgg ccagcccttc gtcaccgcca
      481 gatccggcaa tcgcggatcc ggatcgcgat cgaaggcggg caatcgtggg agcgagagtt
      541 gaccttccgc gagccggtcg gcagccagcg cctgatcgag accctgcgca gccggctgca
      601 ggcgatcgag ctgcccgggc cggccgagac gatccacctc gacctgatcg gcatcgtcaa
      661 cgagatcgcg acccaggagc tgatcccgct gctccggcca aagcgcgatc gcccgttgat
      721 cgaggccgcg cgccagttga aacagcgata tggggagtcc ccgcttgctc acatcgtgga
      781 ggtagagcca tggtcgcgca ttccggaacg ccgattcgcc ctgatcagct acgacccctg
      841 aatcgacctc gccgctgcac ggtccagacc gaccgcggcg ttccgatcgc gatcatcgaa
      901 ggaaaacgac atgagccggt cgagcgggtc caggatcgct ggcagctcaa cgatgagtgg
      961 tggcgcagcc caatcgagcg acactacttc gagctggtga tggcggacgg ccggatccgg
     1021 acggtctacc acgaccgcca gcgggacgag tggttcgagc agcggggatg agcgcctaca
     1081 cggagctgca ccttcacacc tcgttctcgt tcctggacgg cgcctcacag ccggaggagc
     1141 tgattgcccg cgcgctcgag ctcggtttcg attcgctcgc ggtcactgac cacaatggcc
     1201 tctacggcgc gatggagttc gcccagctcg caaatggggt cggtgtgcgg ccgatcaccg
     1261 gtgcggaagt gacgctgctg gacggcacgc acctgaccct gctggccgag aatcagcgcg
     1321 gctacgtcaa cctctgccgg ttgctgaccg aggcgcaccg gctgcgcgat ccgaacccgc
     1381 cctcgcgccg gctcgagatc ccgttcccgc tgcggacagt cggcgtctcc cgcccggccc
     1441 agcgcgacga gacgcccgat ccccggctcg acccgaccct gctggcgacg cacggggagg
     1501 ggatcatcct gctgaccggc tgccggcagg ggcagatcag ccagttgctc gacgacggcc
     1561 agttcgcgct cgcggaggag gtcctgcgcc ggtatgtcga ctggctcgga cccgatcagg
     1621 tcttcgtcga gctccagcac aaccttgtct tcggtgacac ccgtcgcgtc gcgcacatgg
     1681 cccagctcgc cgagcatgtc ggcgtgcggt acgtcgcgac cggcaacgtc cactaccacg
     1741 tcccacagcg gcaccggctc aacgatgttc tggtcgcgat ccggagtcac tccaacctcg
     1801 atggctgcca tcgcgaacgg cggccgaacg accagttcta tctccgttcg gccgacgaga
     1861 tggccgagcg cttcgctgcg tatcccgagg cgctcgccac gacccggcta atctcggagc
     1921 ggtgcgcctc gttcaacctg gccaaggacc tc
//
LOCUS       DATAOZ010000537         3782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1264091, whole genome shotgun sequence.
ACCESSION   DATAOZ010000537 DATAOZ010000000
VERSION     DATAOZ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264091"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2672
                     /locus_tag="VHR64_13650"
     CDS             <1..2672
                     /locus_tag="VHR64_13650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX3303925.1"
                     /translation="LRPILSGKNMLMMTDGERVTAQASGTWSSIFDRDSVTNKLPVLF
                     WYLALQIMALSALPICWRVLSGLPDRGYAVSKAVGLLLVSYFAWLLPSLHILKFGRFA
                     VVVGILFAALASLILIFSKRRAFVADLRSRWREIVFAEGVFLAAFLLFVWFRSMNPDL
                     WQIWRGGEKPMEFAYFNAILRSAHFPPYDPWFAGGYINYYYFGYVLVASVTRLTGIVP
                     AVAFNLAVPTLFALTILNAWSFTANVLRIMTPELKWRSWWRPLVAGLLGPFFVALLGN
                     LDMPLRVARGEYGYPVTTNSGILGIASGVWNSINNRHELPTDAFWASSRIIDGTVNEF
                     PYFTFLFSDLHPHLMAIPFAITALILALGVMRAQKWPAEPANVPAERDEPLYGPSRSW
                     GSILRSIPVRLTSERAILIGLVAFVTGALYPLNTWDYPTYLGLVVGAFALLELINVST
                     SGWKFTFASLRRWVFWSASTLVVGRILFFPYFAHYSQAYSGFTKWTDIKTTPAQYLTI
                     FGLLLFFSISFIVFDLFAAGPGKVRIPFVRPDKLLLSSGNEESSERRVTASITYSTKP
                     VLVRIVLAIVAVLLVVIFAGAFGDNLTQLLGGMLALTAVAAWFRRHDPVRLFIIAMTG
                     AGLAISLAVERYTLRGDIGRMNTVFKFYLEVWVLLALAGAIGTVIILVRYGKALRWPG
                     LELWIAAAVFLVAAGLVYPALATPARLNDRFLDLSPTLNGMAYMPDSTVGDQPENKPP
                     VEMPLIDDYKAINWLLDNVKGSPVILEAQTPEYRWGSRVSIYTGLPTVIGWNWHQRQQ
                     RGGYESLVDQRSAEVKYVYDNVVPFSSIAPILDKYHVKYIYVGDLERAYYDAAGLAKF
                     DQAVADGQLKLVYDNSGVKIYEYGGPTETEATT"
     gene            3096..>3782
                     /locus_tag="VHR64_13655"
     CDS             3096..>3782
                     /locus_tag="VHR64_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303926.1"
                     /translation="MATMTPTRTKRYSFEQFTAIRRYQPALDFSPDGSEIAYSTNTSG
                     QFNLWKQPSNGGYPKQLTVYSDNAVREIAWAPDGEQILFTADHHGDEFTRPYLIPANG
                     GKPEQIPGEPDVRYFLGGIESGSPNGRSFAFSGNDREPTDQDVLVYDTETGETQRPLA
                     GGAFYTPLAWSPDGEYLTVVEARSNTDTVVHLLAVKTREARALLGKDDEDNYIPLDWA
                     ADGSGFWLLTD"
BASE COUNT          672 a         1142 c         1133 g          835 t
ORIGIN      
        1 atcttcgccc gatcctgtcg ggcaagaaca tgctgatgat gaccgacgga gagcgggtga
       61 ccgcgcaagc gagcggcacg tggagcagca tcttcgatcg cgacagcgtc acgaacaagc
      121 tgccggtgct cttctggtat ctcgcgctgc agatcatggc gctatcggcg ctgccgattt
      181 gctggcgggt gctcagcggt ctgccggatc gcggctatgc cgtgtcgaag gcggtcggtc
      241 tgctgctggt cagttatttc gcctggctct tgccgagtct ccacatcttg aagttcggca
      301 ggtttgcggt cgtggtcggc attctgttcg ccgcgctcgc ttctttgatc ttgatatttt
      361 ccaagcgcag agcgtttgtt gccgatttgc ggtcacggtg gcgcgagatc gtcttcgccg
      421 aaggggtgtt cctcgcggcg ttcctgctct ttgtctggtt caggtcgatg aaccccgacc
      481 tctggcagat ttggcgcggc ggcgaaaagc cgatggaatt cgcctatttc aacgcgattc
      541 tgcgcagcgc ccacttcccg ccatacgatc cctggttcgc tggcggatac atcaactact
      601 actactttgg ttatgtcctg gtcgcgtcgg tgacgcgact caccggcatc gttcccgcgg
      661 tcgcattcaa cctggctgtg ccgactttat tcgcgctgac gatcctcaac gcgtggtcct
      721 ttaccgccaa tgtcctgcgg atcatgaccc cggaattgaa gtggcgatct tggtggcgtc
      781 cactcgtggc tggtctgctc ggtccgttct tcgtcgcgct tctgggcaat cttgatatgc
      841 cgttgcgggt ggcgcgcggg gagtatggct atccggtcac gacgaacagc ggcatccttg
      901 gcatcgcctc gggagtctgg aactcgatca ataaccgcca cgagctgcca acagacgcgt
      961 tctgggcgtc gtcgcgcatc atcgacggca cggtcaacga gttcccctat ttcaccttcc
     1021 tcttctccga tcttcatcca cacctgatgg cgattccatt cgcaatcacg gcgctgatcc
     1081 tcgccctcgg ggtgatgcgt gcccaaaagt ggcccgccga gcccgcgaac gtccctgcgg
     1141 agcgagacga gccgctctac ggtccgtcgc ggagctgggg atcgattctg cgctcgattc
     1201 cggtacggtt gacgagtgaa cgcgcgatct tgatcggact cgtcgcgttc gtgaccggcg
     1261 ccctctatcc gctgaatacg tgggactacc cgacctatct cggtttggtc gtcggcgcgt
     1321 ttgcgctgct cgaactgatt aatgtctcaa catcgggctg gaagtttacc tttgcatcgt
     1381 tgcggcgctg ggtattttgg tccgccagca ctctggtcgt tggccgtatc ctcttctttc
     1441 cctatttcgc gcattacagt caggcgtaca gcggcttcac caagtggacc gatatcaaga
     1501 cgacgccggc gcaatatctg acaatcttcg ggctactcct cttcttttcg atcagcttca
     1561 tcgtgttcga tcttttcgct gcaggtcccg gcaaggtcag gatcccgttc gtccggccgg
     1621 acaagctgtt gttatcgtcg ggcaatgagg agtcgagcga acgccgtgtg accgcctcga
     1681 tcacctattc gaccaaaccg gtactcgtcc gaatcgtgct ggcgatcgtg gcggtgcttc
     1741 tcgtcgtgat cttcgcgggc gcgtttggcg acaacctgac gcagctactt ggcggaatgc
     1801 tcgcgttgac cgcggtagcc gcatggttcc ggcggcatga tccagtccgg ctcttcatca
     1861 tcgcgatgac cggtgcggga ttggctatca gtctggcggt cgagcgctac acgctccggg
     1921 gtgacattgg ccggatgaac accgtcttta agttctatct cgaagtctgg gtcctcctgg
     1981 cgctggcggg agcgatcggc acggtcatca ttctggtccg atacggcaaa gcgctgcgct
     2041 ggcctggttt agagctctgg atcgcggcgg ccgtgttcct ggttgccgcc ggactggtct
     2101 atccggccct ggcgacgccc gcccgcctca atgaccggtt cctcgatctc tcaccgaccc
     2161 tgaacggaat ggcgtacatg cccgattcga cggtcggcga tcagccggag aataagccgc
     2221 cggtcgagat gccgttgatt gacgactaca aggcgatcaa ttggctgctc gataacgtta
     2281 agggctcgcc ggtcattctc gaagcgcaga cacctgagta tcgctgggga tcgcgggtca
     2341 gcatctacac tggtctcccg accgtgattg gctggaactg gcatcagcgg cagcagcgag
     2401 gcgggtatga gtcgctcgtc gatcagcgct cggccgaagt gaagtacgtg tatgacaacg
     2461 tggtgccgtt ctcgtcgatc gcaccgatcc tcgacaagta ccacgtcaaa tacatctatg
     2521 tcggcgatct cgagcgcgcc tactacgacg cggccggtct ggccaaattc gatcaagctg
     2581 tcgcggatgg gcagctcaag ctcgtttacg acaattccgg cgtaaaaatc tacgagtacg
     2641 gtggcccgac cgagaccgag gcgacaacgt agagagcgct gagtttcaca ggtcgagcat
     2701 tctcacttgt ggatttccca gccggcgatg gccagaggct gaatcgccca gagggaaggc
     2761 tcaacctaca aagccggctg acgacaggcg tcaggcgtca gaagttaggt ggtaggcgac
     2821 cgtgctcagc ccacggagga gggcttcgca gatcttagcg acggtcttga ggccgtcggt
     2881 gcgcaaccga cggtgtccaa ccgtcggccc tccgtcgtcc cgtctgtcgg cccttagccc
     2941 cgggtaccat ttgggtgcgt tcgccttggg cgggcgcagc ccatgtaacc caaaatccgt
     3001 catttctccg cgcctctggt atcctctacc gccggacgtg gcgagacgtt cgccatcgga
     3061 acggatgcag agtctccagc gcaggagcgt aattcatggc cacaatgacc ccgactcgca
     3121 cgaagcgata ttcctttgag caattcacgg cgatccggcg ttatcaaccc gcgctcgatt
     3181 tctcgccgga tgggagcgag atcgcttact ccacgaatac ctcaggacaa tttaatctgt
     3241 ggaagcagcc gtcgaatggc ggctatccaa agcaactgac ggtctattcc gacaacgcgg
     3301 tccgcgagat tgcatgggca ccggacggcg agcaaatcct cttcaccgcc gatcaccacg
     3361 gcgatgaatt tactcgaccc tatctcatcc cagcgaacgg gggaaagcca gagcagattc
     3421 cgggcgaacc ggacgttcgg tatttcttgg gcgggattga atccgggtcg ccgaacggcc
     3481 gctcctttgc gttctcgggg aacgaccgcg agccgactga tcaggacgtg ctcgtctacg
     3541 acaccgaaac aggcgagacg caacgtccgc tcgccggagg cgcgttttat accccgctgg
     3601 cctggtcgcc ggacggcgaa tatctgaccg tggtcgaggc gcgatcgaat accgacacgg
     3661 ttgtccacct gctcgcggtc aagacccggg aagcgcgggc gctcctgggg aaagacgacg
     3721 aggataacta cattcccctc gattgggcag cagacggatc agggttctgg ctgctcacgg
     3781 ac
//
LOCUS       DATAOZ010000538         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5477069, whole genome shotgun sequence.
ACCESSION   DATAOZ010000538 DATAOZ010000000
VERSION     DATAOZ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5477069"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..503)
                     /gene="greA"
                     /locus_tag="VHR64_13660"
     CDS             complement(39..503)
                     /gene="greA"
                     /locus_tag="VHR64_13660"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX3303927.1"
                     /translation="MRDGAVQLTSAGRSRLEDELAYLRDVKRRELADRLEDSNEDGDI
                     SDNSEYEDLKEDLVMTDRRIQELEYLLATAEPIEPSKDGSVHLGSVVTLVDSDGEEAT
                     WTLVDPAEADTRAGSISTDSPVGRALMGCHKGDSATVETPGGQIVYTVVNVA"
     gene            1090..1302
                     /locus_tag="VHR64_13665"
     CDS             1090..1302
                     /locus_tag="VHR64_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303928.1"
                     /translation="MTNRVRYYITESDDRPIVRSVPVTVSTQPAHDLMVFEKKNDHAE
                     KDRMSTRLPVGRIVKVLRKPSHSTRS"
     gene            1318..>2594
                     /gene="mgtE"
                     /locus_tag="VHR64_13670"
     CDS             1318..>2594
                     /gene="mgtE"
                     /locus_tag="VHR64_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX3303929.1"
                     /translation="MEGVTLSTASSEANLDRLRSAIDQQDGELARVLARQLLDEDAAE
                     LFEGLDRSEIRALAALLGDETLADLLSQLDEHDAADILEQLPVQHAADVLEEINPDDA
                     ADIIVQVDPDVSEDILVAMEPSDAAEIRELMAYRPDSAGGIMTPEFVAMEPGLRADRA
                     IAALRKVAEEAEIIYYVYITDPEEHLLGVLALHRLVLSRPDALVEQLMYPDPVRVNVN
                     DDQEYVARLLTERNFLAIPVVDDENRLVGIVTAEDVADVLEEEATEDIERLGGSAPLT
                     EPYLRASPALLFRKRIVWLLVLFMAQFVTLEVYDHYQGVLEEQLVLAMFVPILIGTGG
                     NVGSQTVTTIIRAMAVGEAAPRHFVRILGKEIVTALGLGAVMGTLMFARGAITNGREF
                     GFTVGLTVGVLIIWAATVGAMLPLVLSKLRVDPA"
BASE COUNT          460 a          816 c          824 g          494 t
ORIGIN      
        1 gcacccggcg gtatcggttt cgatcgctgg ttgcgtagct aagcgacgtt gaccacagtg
       61 tagacgatct gcccgcccgg ggtttcgact gtggccgaat cgcctttgtg gcagcccatc
      121 agcgcccggc cgaccggcga atcggtcgaa atcgacccgg cgcgcgtgtc ggcctcggcg
      181 ggatcgacga gggtccaggt agcctcctcg ccgtccgaat cgacgagcgt cacaaccgat
      241 ccgaggtgga ccgagccatc cttgctcggc tcgatcggct cggccgtcgc cagaaggtat
      301 tcgagctcct ggatgcgccg atcagtcatc acgaggtcct ccttgaggtc ctcgtactcg
      361 ctgttgtctg agatatctcc gtcttcgttg gagtcttcga ggcgatctgc gagctcgcga
      421 cgtttcacgt cacgaaggta cgccagctcg tcctcgagtc gcgatcgtcc cgccgacgtc
      481 agttgaaccg cgccgtcacg catatctggt actccgtttc caatgctgct ccccatggag
      541 cgcacacgcc ggcgtgtgct tcaaggtttc ccggcgcgcg aaccgcaaga tgcattgccg
      601 ggaatgccag ggttcgacat ataagaaaaa gccgaaggcg ggtgccctca gcccattgtc
      661 gcgcgagatg agtatagtcc cgccgacgga aagagtcgac tgtgcaatcg tctgggcgtg
      721 cacaggcaaa agtaccgtcg cacctggata gattcgcgcc ggggtccaag cgtgcgtctc
      781 gggcgctacc tttggcccgt aaataccgtt gacgctcgaa cataaagttc ggtagcatta
      841 gattgccatt gcggaatggc gtgcgcccta ctatgcccgg ctcttgatga gcgggctggc
      901 gcaccaaggc ccagcgaagg gagcggcgtt gaacgccggt agcagtccta gttactcgac
      961 cagggcgcgc cctcttgccg cagactgggg tcctctgagc taagtctcat tcgccaccgt
     1021 ctgacagcat ccgagggacg cgccgaaaac ggcacctcgg atttttgttg tccagaaagg
     1081 cggtggctca tgaccaaccg tgtccgctac tacatcaccg agagcgacga tcggccgatt
     1141 gtgcgcagcg tccccgtgac cgtcagcaca cagccggccc acgacctcat ggtcttcgaa
     1201 aagaagaacg atcacgctga aaaggatcgg atgtcgaccc gtttgccggt cggccgcatc
     1261 gtgaaggtgc tgcgcaagcc gtcgcattcg acccgctcct aacccggacg tctgtccgtg
     1321 gaaggagtca cgctatccac ggcatcgtcc gaggcgaatc tcgaccggct gcgttcggcc
     1381 atcgatcagc aggacggcga actggcgcgc gtgctcgcgc gccagctcct cgacgaggat
     1441 gccgcagagc tctttgaggg cctggatcga tccgagatcc gggccctcgc cgcgctgctc
     1501 ggcgacgaga cactcgccga cctcctcagc cagctcgacg agcatgacgc ggccgatatc
     1561 ctcgagcagc ttccggtcca acacgcggcc gacgtcctcg aggagatcaa ccccgacgac
     1621 gcggcagaca tcattgtcca ggtcgatccg gatgtctctg aagacattct ggtggccatg
     1681 gagccatccg acgcggccga aatccgcgag ttgatggcgt accgcccgga tagcgcgggc
     1741 ggcatcatga ctcccgagtt cgtcgcgatg gagcccggtc tgcgcgcgga ccgggctatt
     1801 gccgcgcttc ggaaggtcgc cgaagaggcg gagatcatct actacgtcta tatcactgat
     1861 cctgaagagc atctgctcgg cgtgcttgcc ctgcatcggc tcgtgttgag ccggcccgac
     1921 gcgctggtcg agcaactgat gtaccccgac ccggtgcgag tcaatgtcaa cgacgaccag
     1981 gagtacgtcg cgcgcctgtt gacggagcgt aatttcctgg caattccggt cgtggatgac
     2041 gagaaccggc tggtcgggat cgtgaccgcg gaggatgtcg cggacgtgct cgaggaggaa
     2101 gcgaccgagg acatcgagcg gctcggcggt tcagcgccgc tgactgagcc atacctccgc
     2161 gcatccccgg cgctgctttt tcggaagcgg atcgtctggc ttctggtcct cttcatggcc
     2221 cagttcgtga cgctcgaggt ctacgaccat taccagggcg tgctcgagga gcagctcgtt
     2281 ttggcgatgt tcgtgccgat cctgatcggg accggcggaa atgtcggctc gcagacggtg
     2341 accacaatca tccgtgcaat ggcggtcgga gaggcggccc cgcgccactt cgtgcggatt
     2401 ctgggcaagg agatcgtcac cgcgcttggt ctcggcgcgg tcatggggac gctcatgttc
     2461 gcccgcggcg cgatcacgaa tgggcgtgag ttcggcttca cggtcggatt gaccgtcggc
     2521 gtgctgatca tctgggccgc gacggtcggc gccatgctgc cgcttgtcct gagcaagctg
     2581 cgcgtggacc cggc
//
LOCUS       DATAOZ010000539         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2055875, whole genome shotgun sequence.
ACCESSION   DATAOZ010000539 DATAOZ010000000
VERSION     DATAOZ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2055875"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VHR64_13675"
     CDS             <1..706
                     /locus_tag="VHR64_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX3303930.1"
                     /translation="KLPRTTVHELLGTLAERRYLVLLPGQPQRYQLGVRLFQLGNIYA
                     DQLDLAREARREAGEVAAACDETVHVAILEGRDVIYIARVDSTHPVRMVSAIGRRLPA
                     HCTGVGKMLLSDQPPEALDALYPRNSTLPVMTPNSISSVSRLRKALIEIGESGLSYDD
                     GESTEDVRCLAAGVRDSSGEMVAAMSISVPTMRWTDAVREELSDLVRAGALRLSGSLG
                     YREPHSTPGMLDARGR"
     gene            956..>1868
                     /locus_tag="VHR64_13680"
     CDS             956..>1868
                     /locus_tag="VHR64_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX3303931.1"
                     /translation="MATSRADATVPTDGPRKSGLRRNSLGILGAVAITAAFMGPSSSV
                     YFGSFAAMSKAGPAYAFSIVLAIIGCLLIAHAVAEFSRKLPTAGMAYTFATATFGPRV
                     GFMTGWLLLIGYIPIVSLLSAAVGILSEQFCKTYFDVSIPWWVFTTMSILAVMYVCNS
                     GVSRSARVILAFLVFEVGVFTALFLTIIVKGGTDGNTLAPFNPGNSLTGTSGIAYGVL
                     WAFWMFFGFEAAGTLGEETKDPRRNVPRALFAAVLIIGSYYVLSAYAAAIGFGVNSGA
                     FATDGSPWDTLSQTYWGGNIGWLILLTV"
BASE COUNT          319 a          588 c          589 g          372 t
ORIGIN      
        1 gaagttgccc cgaacgactg tgcacgagct tctcgggaca ctcgccgagc ggcgctatct
       61 ggttctcctg ccgggacaac cgcagcgcta ccagctcggc gtacgtctgt tccagctcgg
      121 caacatctat gccgatcagc tcgatctggc tcgtgaagcg cgacgtgagg caggcgaggt
      181 tgcagccgcg tgcgacgaga ccgtccatgt ggcgatcctt gaaggccgcg atgtcatcta
      241 catcgcgcgg gtcgacagca cgcatccagt gcggatggtg tccgcgatcg gccgtcgtct
      301 gccagcccac tgcacaggcg tcgggaagat gctcctgtcc gaccagccac ccgaagcgct
      361 cgatgcgctg tacccccgga acagcacact ccccgtgatg acaccgaaca gcatctcgtc
      421 ggtctcccgt ttgagaaagg cgctgatcga gatcggcgag agcgggctgt cgtacgacga
      481 tggcgagtcg accgaggacg tgcgctgcct ggccgccggc gtcagggaca gctccggcga
      541 gatggtggcg gcgatgagca tctccgtgcc gacgatgagg tggacggacg cggtgcgcga
      601 agaactgtcc gacctcgttc gggcaggggc acttcgcctg tcgggaagcc tcggctaccg
      661 cgaaccgcat tcgaccccgg ggatgctgga tgcgcgcggg cgttgatgcg gcagagccgc
      721 ccttagatcg tgcgatgagc cggtacttgt tcggctcgtc gcacagcaac tggcttcggc
      781 ggaacgggtg cgagaccagc ccaccctcag cgagctgaga aggcttgtct gctccggtga
      841 tcctcgcgat cgttgacacc gcccaggcaa gtcaataggg tccaaccgat atacagtatc
      901 gtgttcgata tatcgaacag agcagcctct gtaccttccg atgagcggag cagtcatggc
      961 gacttccaga gctgacgcga cggttccgac cgacggcccc aggaaatccg ggttgcgccg
     1021 gaactccctc ggcatccttg gtgcagtagc gatcactgcg gccttcatgg gcccatcgtc
     1081 gagtgtgtac ttcggcagct tcgccgcgat gtcgaaggcg gggcccgcct atgccttctc
     1141 catcgtgctg gcgatcatcg gctgcttgct gatcgcgcat gccgtggcgg agttcagccg
     1201 caagcttccg actgcgggca tggcctacac gtttgcgacc gcaaccttcg ggccacgagt
     1261 cggcttcatg accggttggc tcctgctcat cggctacatc cccatcgtgt cgctgttgtc
     1321 agcagcggtg ggcatcttgt cggagcagtt ctgcaagacg tatttcgatg tcagcatccc
     1381 gtggtgggtg tttaccacca tgagcatcct cgccgtgatg tacgtctgca actccggcgt
     1441 cagccggtcg gcgcgggtga tcctggcatt cctggtcttc gaggtcggcg tcttcaccgc
     1501 cctctttctc acgatcattg tcaagggcgg gaccgacgga aacaccttgg cacccttcaa
     1561 ccccgggaac tcgctcaccg gaacgagcgg cattgcctac ggtgtcctct gggcgttctg
     1621 gatgttcttc gggttcgaag cagcaggaac gctgggcgaa gaaacaaagg acccgcgccg
     1681 aaacgtcccg cgagccttgt tcgccgcggt gctcatcatc gggtcctact acgttctttc
     1741 tgcgtacgca gctgcgatcg gcttcggggt gaacagcggc gctttcgcga ctgatgggtc
     1801 tccgtgggat accttgtcgc agacctactg gggcggaaac atcggctggc tcatcctcct
     1861 gactgtca
//
LOCUS       DATAOZ010000540         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5477250, whole genome shotgun sequence.
ACCESSION   DATAOZ010000540 DATAOZ010000000
VERSION     DATAOZ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5477250"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1513)
                     /locus_tag="VHR64_13685"
     CDS             complement(116..1513)
                     /locus_tag="VHR64_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3303932.1"
                     /translation="MLQRSNKRRGPEDVTARCLTSNLLTLGRDLRAAGLPIGSGQLIT
                     FVESVGELDCRIRSDFYSAAKATLVNSPEQIPTFDAVFGRFWRQVMNPIAPVETPIEE
                     MLTSDEPPEDSTPRDSSEQEKRAGADNATRVRERTVMAIEDTADEGDGEAEDLDDLPD
                     DVIVFSAREMLRKKDFSQFTADEIAEARRMIAKMDWRLGTRKTRRKERASHGDFIDYR
                     GTLRRSLRNNGVPVELKYRRRKERMRPLVLICDISGSMDRYSRLLLQFVHALEHGLDS
                     VEVFVFSTRLTRITRELRKRNVDAAIEHVVKSVDDWSGGTRIGEAIKDFNFKWARRVL
                     RSGATVVMISDGWDRGDPKLLSKEMARLQRSCRRLIWLNPLLGAPGYQPLTQGIRAAL
                     PYVDYFLPIHNLKSMEALARMLGEVDDQPPVRRQSTVAGTAAEQLPGIARIGNRGELT
                     IEIDPKYLARVNARR"
     gene            complement(1494..2441)
                     /locus_tag="VHR64_13690"
     CDS             complement(1494..2441)
                     /locus_tag="VHR64_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX3303933.1"
                     /translation="MSTTSEALATVHTGRNFDTVDQLEAALRGEKYIADRSLATTLFL
                     TLRLRKPLLLEGEAGVGKTEIAKVLSTILGTPLIRLQCYEGLDASAAIYEWDYPRQML
                     YLRTLEVAGGINTEAARRDIFGEEFLLKRPLLQAIDAAHEQSPVLLIDEVDRADQELE
                     AFLLELLSDFQVTVPELGTIRAEHAPIVILTSNRTREIHDALKRRCLYYWIDFPTYNK
                     EFRILSAKVPDAPEKLRVQICGFTQGLREVDLFKPPGMAETLDWAQALLSLGTTELNP
                     ESVEDTLGVILKYQEDVDRVRGDQAEQLIERAARATAQQ"
     gene            complement(2541..>4050)
                     /locus_tag="VHR64_13695"
     CDS             complement(2541..>4050)
                     /locus_tag="VHR64_13695"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX3303934.1"
                     /translation="TAPETLKTYSLLIDGKSVPGANGKTFDTVSPTTNQPIGRVPLAG
                     VEDAERAIKAARKAFDEGPWPRWTPLERSRALHRVSQFLRDRIDEISTLETMNCGKII
                     VESRADVTASANCFEYYGNLATQLWGEQIPMNGPLLDYTLREPVGVCAQIVPWNFPLL
                     MAAWKLAPALAAGNTLILKPASATPLTAILLGEICRDAGIPDGVVNVITGPGGEIGEV
                     LVGSPLVDKVAFTGETATGRRIMELAAGTIKKVTLELGGKSPNIVFPDADIDEAVNGS
                     LFAIYANAGQRCTARTRLFLHKSIYDDFTRAFVDKARKIRVGDPLDFETQMGPVISPQ
                     QRDRVLNYCQLAVEDGAELLSGGKRVTTSELADGNFVEPTVFGNVRSDMRLAQEEVFG
                     PILAVLPFETEDEVVKAANSTIYGLAATIWTKDIKRAHTMARRLQAGNVSINYPTVNP
                     PEAPFGGYKQSGLGRELSRHVMDLYTQVKNVVVNLNPEPFDWYGRWPTPPKK"
BASE COUNT          657 a         1339 c         1300 g          754 t
ORIGIN      
        1 cgtcgcgccg accggtcgcg gcgcggagcc ttcgactttg acgaccgtcg cgatcgcgat
       61 ccggtcaccc cgggcgcgcc aggcatccac ttcgggcaga aactctctca tcaggtcacc
      121 tccgtgcgtt cacgcgagcc aggtacttgg gatcgatctc gatcgtcagc tcgccgcgat
      181 tgccgatccg ggcgatgccg ggcaattgct cggccgccgt tccagcaacg gtgctctggc
      241 ggcgaaccgg cggctggtca tcgacctcgc cgagcatcct cgccagcgct tccatgctct
      301 tcaggttatg gatcggcagg aagtagtcaa catacggcag cgccgctctg atgccctgcg
      361 tcagcggctg gtatccgggc gcaccgagca gtggattgag ccagatcagc cgtcggcagg
      421 accgctgcag ccgcgccatc tccttcgaaa gcagcttcgg gtcgccacgg tcccagccgt
      481 ccgagatcat gacgacagtc gcgcccgatc gcagcacccg gcgcgcccat ttgaagttga
      541 agtccttgat cgcctcgccg atccgcgtcc cgcccgacca gtcgtcgacc gacttgacca
      601 cgtgctcgat cgccgcatcg acgttgcgct tgcgcagctc ccgcgtgatc cgggtcaagc
      661 gcgtgctgaa gacgaagacc tcgaccgaat cgagtccgtg ctccagcgcg tgcacgaact
      721 gaagcagcaa ccgcgaatag cgatccatcg agccggagat atcgcagatc aggacgagcg
      781 ggcgcatccg ctccttgcgg cgccggtatt tgagctcgac cggcacgcca ttgttccgca
      841 gtgatcgccg gagcgtgccc cggtagtcga tgaagtcgcc gtgtgacgcg cgctccttgc
      901 gccgcgtctt gcgcgtgccg agccgccaat ccattttggc gatcatccgc cgcgcttcgg
      961 cgatctcgtc ggccgtgaac tgcgagaagt ccttcttgcg cagcatctcg cgcgccgaga
     1021 agacgatcac gtcgtccggg agatcatcga gatcttcggc ctccccgtcg ccctcgtcgg
     1081 ccgtgtcctc gatcgccatc accgtgcgct cgcggacgcg ggttgcgttg tccgcgccgg
     1141 cccgcttctc ctgctcgctc gagtctcgcg gcgtgctgtc ctcaggcggc tcgtcgctgg
     1201 tgagcatctc ctcgatcggc gtctcgaccg gcgcgatcgg gttcatcacc tgccgccaga
     1261 accggccgaa gaccgcatcg aaggtgggga tttgctccgg cgaattgacc agcgtggcct
     1321 tggccgctga ataaaagtca ctccggatcc ggcaatcgag ctcgccgacc gattcgacaa
     1381 aggtgatcag ttggccgctt ccaatcggca aaccggccgc gcggagatcc cgccccagcg
     1441 tgagcaggtt actggtcagg caccgggccg taacgtcctc gggaccgcgt cgcttattgc
     1501 tgcgctgtag cacgggccgc ccgctcgatc aattgctcgg cctgatcgcc acgcacgcga
     1561 tcgacgtctt cctggtactt gaggatgacg cccagcgtgt cctcgaccga ctcggggttc
     1621 aactcagttg ttccgagaga gagcagagcc tgtgcccagt cgagcgtctc ggccattccc
     1681 ggcggcttga acaggtcgac ttcgcgcaac ccctgcgtga agccgcagat ctggacgcgc
     1741 agcttctccg gagcatccgg caccttggcc gagaggatgc ggaactcctt gttgtaggtc
     1801 gggaagtcga tccagtagta gagacagcgc cgcttgagcg cgtcgtggat ctcgcgcgtc
     1861 cggttggagg tcaggatcac gatcggcgcg tgctcggccc ggatcgtacc cagctccggc
     1921 actgtcacct ggaagtccga cagcagctcg agcaggaacg cctccagctc ctggtccgcg
     1981 cggtcgacct cgtcgatcag gagcaccggc gactgctcgt gcgcggcgtc gatcgcctgc
     2041 aggagtggcc gcttgagcag gaactcctcg ccgaagatgt cgcgccgggc ggcctcggtg
     2101 ttgatgccgc ccgcgacctc gagggtccgc agatagagca tctggcgcgg gtaatcccac
     2161 tcatagatcg cggcgctggc gtccagaccc tcatagcact ggagccggat cagcggtgtg
     2221 ccgaggatcg tcgagaggac cttggcgatc tcagtcttgc cgacgccggc ctcgccctcg
     2281 aggagcaacg gcttgcgcag gcgcaacgtc agaaagagcg tcgtcgccag gctgcggtcc
     2341 gcgatgtatt tctcgccgcg cagggcggct tcaagctggt cgaccgtgtc gaagttccga
     2401 ccggtgtgga ccgtggcgag tgcctcgctt gttgtactca agtatcagtt ctccggaaga
     2461 aaggaccggc ggccctcggc atacttttca aatggaagta tcgccgaggg agcggccctc
     2521 tcgccacagg gcgctacggc ttacttcttc ggcggcgtgg gccagcggcc gtaccagtcg
     2581 aacggctcgg ggttgaggtt gaccaccacg ttcttgacct gcgtgtagag gtccatcacg
     2641 tgccggctca gctcccggcc aagaccgctc tgcttgtatc cgccgaatgg agcctcaggc
     2701 gggttgaccg tcggatagtt gatcgagacg ttgcctgctt gcagccgccg cgccatcgtg
     2761 tgggcgcgct tgatgtcttt cgtccagatc gtcgcggcca gcccgtagat ggtgctgttg
     2821 gccgccttga cgacctcgtc ctcggtctcg aacggcagaa cggcgaggat cggcccgaag
     2881 acctcttctt gcgccagccg catgtccgac cgaacattgc cgaagacggt cggctcgacg
     2941 aagttgccgt cagccagctc ggatgtcgtc acccgcttgc cgcccgaaag cagctcggcg
     3001 ccatcctcga ccgcgagctg gcaatagttg agcacgcgat cccgctgctg gggcgagatg
     3061 accggtccca tctgcgtttc gaagtcgagc ggatcgccga cccggatctt gcgcgccttg
     3121 tcgacgaatg cacgcgtgaa gtcgtcgtag atgctcttgt gcaggaagag ccgcgtgcgg
     3181 gcggtgcagc gctgaccggc attggcatag atcgcgaaga gcgagccgtt gaccgcctca
     3241 tcaatgtccg cgtcggggaa gacgatgttt gggctcttgc cgccgagctc cagcgtgacc
     3301 ttcttgatcg tgccggcggc cagctccatg atccgccgcc cggtcgcggt ctcgccggtg
     3361 aaggcgacct tgtcaaccag cgggctgccg accagcacct cgccgatctc gccgcccgga
     3421 ccagtgatca cgttgacgac gccgtccgga atgccggcgt cgcggcaaat ctcgccgagg
     3481 agaatcgccg tcagtggcgt ggccgaggcc ggcttgagga tgagcgtgtt gcccgctgcc
     3541 aatgccggcg ccagcttcca ggccgccatc agaagcggga agttccacgg cacgatctgg
     3601 gcgcagaccc cgaccggttc gcgcagggtg taatcgagca gcggaccgtt catcgggatt
     3661 tgctcgcccc agagctgggt cgcgagattg ccgtagtatt cgaagcaatt ggctgaggcg
     3721 gtcacatcgg cacgcgactc gacgatgatc ttgccgcaat tcatcgtctc gagggtcgag
     3781 atctcatcga tccgatcgcg caggaattgc gagacccgat gcagcgcgcg ggatcgctcg
     3841 agcggcgtcc agcgcggcca tggtccctca tcgaacgcct ttcgtgcggc cttgatcgcg
     3901 cgctcggcgt cctcaacgcc cgccagcggc acgcggccga ttggctgatt cgtggtcggg
     3961 ctgacggtat cgaacgtctt gccgttcgcg cctggaaccg acttgccgtc gatcagcagc
     4021 gaataggttt tcagggtctc gggtgcggta
//
LOCUS       DATAOZ010000541         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1528045, whole genome shotgun sequence.
ACCESSION   DATAOZ010000541 DATAOZ010000000
VERSION     DATAOZ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528045"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VHR64_13700"
     CDS             <1..212
                     /locus_tag="VHR64_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303935.1"
                     /translation="LVLLAGTCLAIYSLSRRHRITHANVTDSHEVLVLAAAAPTTVYS
                     DDPRLKALPKPKKKKGKKPKLKSAA"
     gene            212..442
                     /locus_tag="VHR64_13705"
     CDS             212..442
                     /locus_tag="VHR64_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303936.1"
                     /translation="MHIDWAALAIVAVVSIMTSVIFTVLLASGIRLVSAAKIKSNEGG
                     SGTATVSLGYLSLSIAALIVLFGIYLIVPQFH"
     gene            complement(536..1252)
                     /locus_tag="VHR64_13710"
     CDS             complement(536..1252)
                     /locus_tag="VHR64_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3303937.1"
                     /translation="MVHVHGFGISGTYLEPTAALLAPHYRTFVPDLPGMGRSIRPEGG
                     LDLPGLAKALMSYCDAVGVERPILVGNSLGCPVIVEVATTFPDRIQRAVLVSPAGGPN
                     NQPLGRAIGQMAMDGLREPPSMLPIATRDYLRFGVLQSLSLFRAMTRYPTLERLPHLV
                     VPTLVIAGLRDPLVRIANAPRFAVLPHVDAVSVPGAHALNYSAPQLIAELIEAYIAGE
                     PLVTQTGPRSVAAAISVDGN"
     gene            complement(1360..>1539)
                     /locus_tag="VHR64_13715"
     CDS             complement(1360..>1539)
                     /locus_tag="VHR64_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303938.1"
                     /translation="PPQWSLKKEFDMPLATRRMARASVNGPAPVPRTATTVATAAVVG
                     PSMTDRFMDEIETRA"
BASE COUNT          294 a          455 c          493 g          297 t
ORIGIN      
        1 tactggtgct actggctggg acctgtctgg cgatctactc cctctctcgc cgccacagga
       61 tcactcatgc caatgtcacc gacagccacg aagtcctggt gctggctgcc gctgcgccca
      121 ccaccgtgta ctccgatgac ccgcggttaa aggctcttcc gaagcccaag aagaagaagg
      181 gcaagaagcc caaattgaag agcgccgcct gatgcatatc gactgggctg ccttggccat
      241 cgtggctgtc gtctccatca tgacgtcggt gatcttcacc gtcttgctgg cgagcggcat
      301 ccgtttggtc tcggcggcca agatcaagtc gaacgagggc ggttctggta cggcgaccgt
      361 ctcgctgggc tatctctcgc taagcatcgc cgcgctgatc gtgctctttg ggatctacct
      421 gatcgttccg cagttccatt agagggcacg ttgatcgttg atctttattc tgcgtggggc
      481 gcgggagccg tccctccggt ttgtggtggt taagggcgat ttcaggtcga agtagctagt
      541 tgccatcaac gctgatggcg gcggcgacag atctcggccc ggtctgggtt acgagcggct
      601 caccagcgat ataggcctcg atgagctcgg cgatcagctg cggagcgctg tagttgagtg
      661 cgtgtgcacc ggggacgcta actgcgtcga cgtgaggcag aaccgcgaag cgaggggcat
      721 tggcgatccg gacaagtgga tcacgcaaac cggcgatcac gagcgttgga acgacaaggt
      781 gcggaaggcg ttccagtgtc gggtagcggg tcatcgcccg gaacaacgac aagctttgaa
      841 gtaccccgaa ccggaggtag tcgcgtgttg cgatgggcaa catggagggc ggctcacgca
      901 gcccgtccat ggccatttgt ccaattgccc ggcctaatgg ctggttgtta gggccgccgg
      961 ccggcgagac gagcaccgcg cgctggattc ggtcgggaaa ggtcgtcgca acctcgacga
     1021 tgactgggca gccgagcgaa ttcccgacca atatcggccg ctcgaccccg acggcgtcgc
     1081 agtaggacat cagcgccttc gccagacccg gtaagtcgag tcctccctcg ggcctaatac
     1141 tgcgtcccat ccccggcaga tcgggtacga acgtgcggta atgcggcgca agaagagccg
     1201 ccgtgggctc caaataggtc cccgagattc caaatccgtg gacatgcacc atcgcatccg
     1261 catcagagcc agcgtcgagg cagaaacgat gaaacaccgg cagtccatca acgatgaccc
     1321 atccgggtgt gagcggaggt acgggtttcc ggggcacggt caggctcgcg tttcgatctc
     1381 gtccataaag cggtcagtca tcgatggtcc gacgacggcg gctgtcgcaa ccgtggtggc
     1441 agtccttggg accggtgcgg gaccgttaac ggacgcgcga gccatcctgc gcgtggcgag
     1501 tggcatgtca aactccttct tcaagctcca ttgcggcgg
//
LOCUS       DATAOZ010000542         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4688100, whole genome shotgun sequence.
ACCESSION   DATAOZ010000542 DATAOZ010000000
VERSION     DATAOZ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4688100"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /gene="glyA"
                     /locus_tag="VHR64_13720"
     CDS             complement(<1..1099)
                     /gene="glyA"
                     /locus_tag="VHR64_13720"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX3303939.1"
                     /translation="MVYAPTDVLTDSEVAAAIRREEIRQRDGIELIASENFVSRNVMA
                     AVGSVLTNKYAEGYPGKRYYGGCEFIDEVETLAIERAKLLFGAEHVNVQPHSGANANL
                     GAYLAIAEPGSRILGLSLSEGGHLTHGHPVNFSGRLFEAHFYTLDHATARIDYDSAEK
                     VAKEIRPAIIIAGASAYSRHIDYARFRQIADEVGAFLVADIAHPAGLVAAGLHPSPIG
                     HAHITTTTTHKTLRGPRGGMIMSSEELGKDIDKSVFPGMQGGPLEHVIAGKAVAFGEA
                     LQPSFKAYAAAVIENAQAMADELISLGMSIVSGGTDTHLMLVDITTLDMSGKKAERIL
                     DSVGITANKNAIPNDPRPPMQTSGIRLGSPAV"
     gene            1213..2196
                     /gene="galE"
                     /locus_tag="VHR64_13725"
     CDS             1213..2196
                     /gene="galE"
                     /locus_tag="VHR64_13725"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX3303940.1"
                     /translation="MRILITGGAGYIGSVAVEQSLAAGHDVVVVDSLWRGHRGAVPDD
                     VELRECDLRDKEGIACVVNEVKPDAIMHFAAAIVVPESVEDPLLYFDVNTVGSHYLIT
                     AAINAGVPRFIFSSTAAAYGNVDAEVLTEDMPTNPISPYGRSKVMVEQMLHWHQQRYG
                     LNVAIFRYFNVAGATERRGEDHDPETHLIPVALQAASGKRDTFTVFGTDYATPDGTCI
                     RDFVHVADLADAHLLAANWLSSNDWGIFNLGTTNGLSVQQIVQAVEGVTDRTLPVTVG
                     PRREGDAARLIASAERARSILGWNPKRSTVEQMVGSAWDWMRHHPNGYGDR"
     gene            2208..3692
                     /locus_tag="VHR64_13730"
     CDS             2208..3692
                     /locus_tag="VHR64_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3303941.1"
                     /translation="MSITNPSAPGTEIDHEAGGAALQLPPDEMRTFGYAVVDSIVDHL
                     ASLREQPTARWADRPTMDARLWQSAPEHATDPREVLDRTVRDVLTDGHRNSHPRYFGY
                     VPNPSNYVGAMADALASGFNIFSGMWMGSPGAAETELLVTDWMRDWCGLPDSGGGILL
                     SGSSMANLVGIATARYARFGHDAEAARRGTIYYSDQAHSSVAKGARMIGVGLDRMRKV
                     PSDDDFRLSMDSLAETVRSDRAAGLEPYCVIAAAGTTNTGAIDPLPELADFCAAEGIW
                     LHADGAFGAAAVLSQNGKRWLAGLERVDSIALDAHKWLFQPIECGCLLVRDVRTLREA
                     FYERPEYLRDADTADAEVNFSDMGPQLTREFRALKLWMSIQVFGMEAIRAAVERGIAL
                     AELAEREIRDAGIWEMITPAQISVLTFRYAPSNLTEQQINRLNARIAHATLEDGYAAL
                     STTELRGKTVLRMCITNPRATEAEIRETVRRLGVYAADCLAEIG"
BASE COUNT          651 a         1199 c         1245 g          665 t
ORIGIN      
        1 tgaccgccgg cgagccgagc cgaatgccgg aggtctgcat gggtgggcgc gggtcgttcg
       61 ggatcgcgtt cttgttggcg gtgatgccga ctgaatcgag gatccgctcg gccttcttgc
      121 cgctcatgtc cagcgtcgtg atatcgacca gcatcaaatg cgtgtcggtt ccgcccgaga
      181 cgatcgacat gcccagcgag atcagctcgt ccgccatcgc ctgcgcgttc tcgatcacgg
      241 ccgcggcgta cgccttgaag gacggctgaa gcgcctcgcc gaacgcgacg gccttgcccg
      301 cgatgacgtg ttcgagcggg ccaccctgca tgcccgggaa gacgctcttg tcgatgtcct
      361 tgccgagctc ctcggacgac atgatcatgc cgccgcgcgg gccgcgtagg gtcttgtgcg
      421 ttgtcgtcgt cgtgatgtgc gcgtggccga tcggtgacgg atgcagacca gccgctacga
      481 gcccggccgg atgcgcgata tccgcaacca ggaacgcgcc aacctcatcg gcgatctggc
      541 ggaagcgcgc ataatcgata tggcggctgt aggcgctggc gccggcgatg atgattgccg
      601 ggcgaatctc cttcgccacc ttctcggcac tgtcgtaatc gatccgggcg gtcgcgtggt
      661 ccagcgtgta gaagtgtgcc tcgaatagac ggcccgagaa attgaccggg tgcccgtggg
      721 tcaggtggcc gccttcggag agcgagagcc cgaggatcct gctgcccggc tcggcgatcg
      781 ccaggtacgc gcccaggttg gcgtttgcgc ccgagtgcgg ctggacgttg acgtgctcgg
      841 cgccgaagag cagcttggcc cgctcgatcg ccagcgtctc aacttcgtcg atgaactcgc
      901 aaccgccgta gtaacgcttg cccggatacc cctccgcgta cttgttggtc aggacggagc
      961 cgaccgccgc catcacgttg cggctgacga aattctcgga ggcgatcagc tcgatgccgt
     1021 cgcgctggcg gatctcctcg cgccggatcg ccgcggcaac ctcggagtcg gtcagaacat
     1081 ctgtgggcgc gtacaccatg ccgggagtcc ttttctgggt gggatctggc gcgaaaacga
     1141 ggcggtagac tgcccgctag tttaccatcc agacagcgag attcagtcag ccagacaggc
     1201 agcgaggttt acgtgcggat attgataacg ggcggcgccg ggtacatcgg aagcgtcgcg
     1261 gtcgagcaat cgttagcggc gggtcacgat gtcgttgtgg tggacagcct ctggcgtggt
     1321 caccggggcg cggtgccgga cgatgtcgag ctccgggagt gcgacctccg ggacaaggag
     1381 gggatcgcct gcgtcgtcaa cgaggtcaag ccggacgcga tcatgcactt cgcggcggcg
     1441 atcgtggtcc ctgagtcagt cgaggatcct ctcctctact tcgacgtcaa cacggttggg
     1501 tcgcactacc tgatcacggc ggcaatcaat gccggcgtgc cgcgcttcat tttctcgtcg
     1561 accgccgcgg cctacggcaa cgtcgacgcg gaggtgctga ccgaagacat gccgactaac
     1621 ccgatcagcc cgtatggccg atccaaggtg atggtcgagc aaatgctgca ctggcatcag
     1681 cagcgatacg ggctgaacgt cgcgatcttc cgctacttca acgtggccgg cgcgactgag
     1741 cggcggggcg aggaccacga cccggagacg cacctgattc cagtcgccct ccaggccgca
     1801 tccggcaagc gcgacacgtt cacggtcttc ggcaccgatt atgcgacgcc agacggcacc
     1861 tgcattcgcg acttcgtgca cgtggctgat cttgccgatg cccatctcct ggccgccaac
     1921 tggttgtcgt ccaacgattg gggcatcttc aacctcggga ccaccaacgg cctgtcggtg
     1981 cagcagatcg tgcaggcggt cgaaggcgtg accgaccgta ccttgccggt aacagtcgga
     2041 ccgcgccggg aaggcgacgc ggcccgattg attgcgtcgg ccgagcgtgc ccgctcgatc
     2101 ctcggctgga acccgaaacg ctcgacggtg gagcaaatgg tcggatctgc gtgggactgg
     2161 atgcggcatc atcccaacgg ctacggtgac aggtagagag cgatattatg tccattacca
     2221 acccatcggc tccaggcaca gagattgacc acgaggccgg cggggctgcc ctgcagctcc
     2281 cgccggatga gatgcgcacc ttcgggtatg cggtggtcga ctccatcgtc gatcacctgg
     2341 ccagcctccg cgagcaacca accgcacgct gggccgaccg tccgaccatg gatgcccgct
     2401 tgtggcagtc ggctcctgaa catgccaccg atccgcgaga ggtgctggac cgcacggtgc
     2461 gcgatgtcct gacagacgga caccgcaaca gccacccgcg ctatttcggg tacgtgccga
     2521 atccgagcaa ctacgtcggc gcgatggcag atgcgctggc atcggggttc aacatctttt
     2581 ccggcatgtg gatgggctca ccgggcgcgg ccgagaccga gttgctagtg accgattgga
     2641 tgagggactg gtgcggactg cccgacagcg gcggcggcat tctgcttagc ggctcgtcga
     2701 tggcgaacct ggtcggcatc gcgaccgctc gctatgcccg cttcggtcac gatgccgagg
     2761 cagccaggcg cggcaccatc tactactcgg accaagcgca ctcgtcggtc gctaaaggcg
     2821 ctcgcatgat cggcgtcgga ctggaccgga tgcggaaagt gccatcggac gacgacttcc
     2881 gcctctcgat ggactcgctc gcggagactg tcaggtccga tcgcgccgcc ggcctggagc
     2941 cgtactgtgt catcgccgct gcaggaacca ccaacaccgg cgcaattgac ccgttgccag
     3001 agttggccga cttctgcgcg gccgagggca tctggttgca tgccgatggc gcgttcggag
     3061 ccgccgccgt gctcagccag aacggcaagc gctggctcgc cgggctcgaa cgggtcgatt
     3121 caatcgcgct tgatgcccat aaatggctct tccagccgat cgaatgcggt tgtctgctgg
     3181 tccgcgatgt tcgcacgctg cgcgaggcgt tctacgaacg gcccgagtat ctccgcgatg
     3241 ccgacacggc cgatgccgag gtcaacttca gcgatatggg gccgcagctg acgcgggagt
     3301 tccgggcgct caagctctgg atgtcgatcc aggtcttcgg gatggaggca atccgcgcgg
     3361 ccgtcgaacg agggatcgcc ctggctgagc tggcggagcg cgagattcgg gacgcgggga
     3421 tttgggagat gatcacgccg gcgcagatca gcgtcctcac cttccggtac gccccatcaa
     3481 atctgaccga gcagcagatc aaccggctca atgcccggat tgcccacgcg acgctcgagg
     3541 acggctacgc ggcgctgagc acgacggaac tgcggggcaa gacggtcctc cgcatgtgca
     3601 tcacgaaccc acgcgcgacc gaagccgaga tccgcgagac cgtgcgtcgg ctgggcgtct
     3661 acgctgccga ttgcctcgcc gagatcggct gagcgccgtc gagatcaatg cggcccttga
     3721 cgtagcgcgg ggttaccatg ccccgcttgg cagttcacca
//
LOCUS       DATAOZ010000543         3514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4158723, whole genome shotgun sequence.
ACCESSION   DATAOZ010000543 DATAOZ010000000
VERSION     DATAOZ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4158723"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VHR64_13735"
     CDS             complement(<1..559)
                     /locus_tag="VHR64_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303942.1"
                     /translation="MSMRPWLDLFRARLREPADLPTLVHELEQVVRAIVQPRPALFWG
                     PPDAAQHWSNPATLPAYATDDPLIEHFVAHPSARRANRIEIDSPVLAALDANVLVPLV
                     SLGTFVGLIAVPVPELSADQIATLDEIAGLAASAIRVARLTHRVDLAERAHQTTTEEL
                     RVAEIIQRSLLPESLPNIAGWRLDAW"
     gene            716..3298
                     /locus_tag="VHR64_13740"
     CDS             716..3298
                     /locus_tag="VHR64_13740"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3303943.1"
                     /translation="MTGSPPLPFPLTPLIGREVELTELDDQLRRPSVRLVTLTGPGGV
                     GKTRLALHVAGRLRDAFPDGVWLVRLEAIREVELVLATIAQAVGIADASASDLAEHIA
                     SWLGPGRHLFLIDNFEQVAEASPVLADLLQRCPGLKLLVTSRTSLGVYGEHDYPLGPL
                     PLPASVDGSGVAGLLAYPAVKLFVDRATEVKPSFALTPENAADVLAICQRLDGLALAI
                     ELAAARIKVLTPAALRERLANRLRVLTSGARSLPARQQTMRGAIAWSYELLSADDRRV
                     FRLLGVFSGGFGLDALETVVERRNGAGGAELIDLDLLLEQMTDLVDHSLVRRVDHGAD
                     EPRFALFQTIQEYATDLLEEAGEGVESRRAHGEWVAALVLRAEPQLTGPDQMIWFERL
                     ETEHDNIRAALTFAIHQQEADLGQRIGGHLWRFWWLHGHLTEGSRWLEQVLALPGEVP
                     PSTRGYAVHALAAMAEELGNFEVAIPGYERALDIAREIGDEHLQAHANTALGNVGLAR
                     GNYDESVSRSRDALALFERLGDRRGIAGCHHNIATVAYHRGDLEAAEAGYRHAMEELA
                     AIGDERSRVMILANIGVIAFERRDFETAQQIQEEAAATMRSMGDEIGYCNSLANLAGV
                     LIELGDSARAMELNRQALEIAERIGLKRNLGYIRSGIGQQLRDRGENAEAAQYLLASL
                     VDLQECGDQAAVANVLGTLAEMAHTLGDSILGARLFGASRGIRDAIGTSHAPDQVEDY
                     ERVLGLVRTGAGDEAFEAAWAAGMTAPIEETVRDAACITELASAAPRDPRDVELEQKT
                     GLTAREITVIRQMVAGKSNQEIAATLSLNLGFVATIIGQIYTKLGVDSLADVTAYAFK
                     NGLV"
BASE COUNT          640 a         1027 c         1271 g          576 t
ORIGIN      
        1 accaggcatc gagccgccag ccagcgatgt tcggcagcga ttcgggcagg aggctgcgct
       61 ggatgatctc ggccacgcgc agctcctcgg tcgtggtctg atgcgcgcgc tcggcgagat
      121 cgacgcggtg cgtgaggcgg gcgacacgga tggcagatgc ggccagcccg gcgatctcgt
      181 ccagggtcgc gatctggtcg gctgacagct cgggcacggg gacggcgatc aggccgacga
      241 aggtgccgag cgagacgagc gggaccagga catttgcatc caacgcggcg agcaccggcg
      301 agtcaatttc gatgcgattg gcgcgacgcg cggacggatg agcgacgaag tgctcgatca
      361 gcgggtcgtc ggtcgcatag gccggcaggg tcgcggggtt ggaccagtgc tgcgcggcgt
      421 cgggcggtcc ccagaatagg gcggggcggg gctggacgat ggcgcggacg acctgctcca
      481 actcatggac gagcgtgggg aggtcggccg gctcgcggag ccgggcgcgg aagagatcga
      541 gccagggacg catcgacaac ggttcagcct atggtgaatg cccgcaggac gggcacggcg
      601 acatgatcac gtatgatagt tcgcacgatc gaaatatcct ttggccaatc gggatgaagg
      661 tggcgcacct cgcatccaaa cacgctggat caatttcgac gtatgcagtg atgcaatgac
      721 cggttcgcca cccctgccat ttccgttgac tcccctgatc gggcgcgaag tcgagctgac
      781 ggagctcgat gatcagctgc gccgtccgtc cgtgcggctg gtcacgctga ccggtcccgg
      841 tggggtcggc aagacgcggc tggcgctgca cgtggccggc cggttgcggg atgccttccc
      901 ggacggtgtg tggctggtcc ggctggaggc gatccgcgag gtcgagctgg tgctggcgac
      961 gatcgcgcag gcggtcggga ttgccgacgc ctcggcgagc gatctggccg agcacatcgc
     1021 gagctggctt gggccgggcc ggcatctctt cctgatcgac aatttcgagc aggtggccga
     1081 ggcgagcccg gtgctggccg atctgttgca gcgatgtccg ggcctgaagc tgttggtgac
     1141 cagccgaaca tcgctcggtg tctacggcga gcatgattac ccgctcggcc cgctcccatt
     1201 gccggcatca gtcgatggat cgggcgtcgc ggggttgctt gcatacccgg cggtcaagct
     1261 gttcgtggac cgcgcgaccg aggtcaagcc gtcctttgcg ctgactcccg aaaacgcggc
     1321 cgatgtgctg gcgatctgcc agcggctgga cggtctcgcg ctggcgatcg agctggccgc
     1381 ggcgcggatc aaggtcctga cgccggccgc gctgcgggag cgacttgcga accggctgcg
     1441 ggtgctgacc agcggcgcgc ggagcttgcc ggcccggcag cagacgatgc gcggcgcgat
     1501 cgcctggagc tacgaactgc tcagcgctga tgatcggcgg gtgttccggc tgctgggcgt
     1561 cttttcgggc ggcttcgggc tcgatgccct tgaaacggta gtcgagcgac gcaacggggc
     1621 cggaggcgcg gaactgatcg atctcgacct cctgctcgag cagatgaccg acttggtcga
     1681 tcatagcctg gtgcgacggg tggaccacgg tgcggatgag ccaaggtttg cgctgttcca
     1741 gactattcag gagtacgcca ccgatcttct cgaggaagcc ggagagggcg ttgaaagccg
     1801 ccgggcgcac ggggagtggg tggcggcgct ggtcctgcgg gcggagccgc agctgaccgg
     1861 cccggaccag atgatctggt ttgagcggct ggagaccgag catgacaaca ttcgcgccgc
     1921 actcaccttc gcgatccacc agcaggaggc cgacctggga cagcggatcg gcggacacct
     1981 ctggcggttc tggtggctgc acggccacct gaccgagggg agtcgctggc tggagcaggt
     2041 gctggcgctg ccgggcgaag tgccgccgtc gacccgtggc tatgccgtgc atgcgctggc
     2101 ggcgatggcc gaggagttgg gcaatttcga ggttgcgatt ccgggatacg agcgcgcact
     2161 cgacattgcc cgggagattg gagacgagca cctgcaggcc catgcgaaca ccgcgctggg
     2221 caatgtcggg ttggcgaggg gcaactatga tgagtcggtc tcacggagcc gcgacgcgct
     2281 agcgctgttc gagcggttgg gagaccggcg tggaattgcc gggtgccatc acaacatcgc
     2341 cacggtggcc taccaccggg gcgacctgga ggcagccgag gccggatacc ggcacgcgat
     2401 ggaggaactc gcggcgatcg gagatgagcg atcccgcgta atgattctgg ccaatatcgg
     2461 cgttatcgcc ttcgagcggc gcgatttcga gacggcgcaa caaatccagg aagaggccgc
     2521 cgcgacgatg cggtcaatgg gcgacgaaat cgggtattgc aactcgcttg cgaacctggc
     2581 gggtgtgctg attgagctcg gcgactccgc gcgggccatg gagctcaatc ggcaggcgct
     2641 ggagatcgcg gagcggatcg gtctcaagcg caatctcggt tacatccggt ccggtatcgg
     2701 acagcaactt cgcgatcgcg gcgagaacgc cgaagccgcg cagtatttgc tcgcatccct
     2761 ggtcgatttg caggagtgcg gcgatcaggc cgcggtggcc aatgtacttg gtaccctcgc
     2821 ggagatggcc catacgctgg gcgattccat tctcggcgct cggttattcg gcgcatcgcg
     2881 aggcatccgc gacgcgatcg gcacgagcca cgctccggat caggtcgagg actacgagcg
     2941 cgtattgggt ctggttcgca cgggggcggg cgatgaggca ttcgaggcag cctgggccgc
     3001 aggtatgaca gccccgatcg aggaaaccgt gcgggatgca gcctgcataa ccgaactcgc
     3061 gagcgcggcg ccgcgggacc cgcgcgacgt ggagctcgag cagaagacgg gtctgaccgc
     3121 gcgggaaatt acggtcatcc ggcagatggt cgccggaaaa tcgaaccagg agatcgccgc
     3181 cacactcagc ctgaacctgg gtttcgtcgc gacgatcatc ggccagatct acaccaaact
     3241 cggcgtcgat tccctcgcgg acgtgacggc atacgcgttc aagaatgggt tggtatgagt
     3301 cgaggagtcg agaagtcgag aagaagtcga gcaggcgagc agtcggaaaa cggaaaacga
     3361 aaaaccaacg acgagagacg aaagacccaa ccagaaactg gaaacgagaa actggaaact
     3421 cgtcactgac cactgatgac cacacattcc gccattccca ttcccatgac gccgttgatc
     3481 gggcgcgata tcgagctgat cgatgtcgag gtgt
//
LOCUS       DATAOZ010000544        10748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3109770, whole genome shotgun sequence.
ACCESSION   DATAOZ010000544 DATAOZ010000000
VERSION     DATAOZ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10748
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3109770"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1681)
                     /locus_tag="VHR64_13745"
     CDS             complement(287..1681)
                     /locus_tag="VHR64_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3303944.1"
                     /translation="MAATVQSPNTVEIDVEALVPMIRGQIIEPGSPTYDEARKTYNGM
                     IDRYPAVIVRAADDSDVISTVRFAKVRNLDLSVRGGGHNVAGFGTNDGGVVLDLSLMR
                     NIRVDPKTSTARAGGGATWADMDHAGHAYGLATPGGVLSTTGIGGLTLGGGFGYLTRR
                     YGLSADNLISADVVTADGDRLTASADENPDLFWAIRGGGGNFGVVTSFEFRMHPVGMV
                     YGGPIFFPVDASAQVLRFYRDFVASAPREMSAFFAYHVAPPAPFIPEALHGHTACAIV
                     ICYTGPMESAEEAVRPIREAGPVALDLAGPIPYPALNSLFDALLPAGLQHYWKADFDY
                     ELTDEAIRIHAEHGAQVPNFMSLMHLYPLDGAVQDVAKDATAFSHRDVKFAHIIAGID
                     TDPANMPARRDWVRAYWSALHNESAGGAYLNFLMDEGQDRIRATYRDNYARLLDVKRT
                     YDPDNVFHVNQNIR"
     gene            complement(1904..4867)
                     /locus_tag="VHR64_13750"
     CDS             complement(1904..4867)
                     /locus_tag="VHR64_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3303945.1"
                     /translation="MGRGDEQELLRADLAAARDGRGGLIVLSGEAGIGKTSLARELVY
                     EATTAGVCVLIGHCYDLTVTPPYGPWLGLAATYRPANGMPLLPEALATGRIDEIESQA
                     ALFADVRSFFAALASVQPVLVVLEDLHWADPASIELLRSLGAAVAAQPILVLVTYRSD
                     ELTRTQPLYHQLPSLLRETGGRRLDLRPLAAPDLRSLVHGNYAMSEEEGERLVAYLER
                     HAEGNPFFVVELLRALSEEGFLRPDGERWSLGELDRIVVPSLLRQVIDARISRLGDET
                     RGPLSVAAVIGHDIPLDLWERVLGLDEEQLLTIVERAIDAHLLNAAPDGTRVRFVHAL
                     TREALYEAVMPPRRRLWHQRVGDALAALPRVSPDEVAFHFERAGDPHAWEWYVRAGER
                     AQRAYAWLMAKDRFVAAAALIENVPGMEARRSRLLYRSGRLQRYSEISDGIVNLAAAE
                     RAANQAGNRVLAADAKYSRGLLRCFSDDFGPGLVEMESGIVELEGMTSDQQMESWERA
                     TWMVDALPPRELADLPDIEPSARIMIAAGTHHRRGGHPWFLAAAGHLARAKAMAEKFI
                     ALTAGIPAGELVISATGHSWFGLGLANAALGRPDEARQAFSRARQIYDLIEHHAVIGF
                     TQLSELRDVVLPYFATDVAGRARLAMDAEISLNLAAGAFPSDRSSRRARLGLLFLEGR
                     WDEADAIASEVEADGNYVLRREITNAMTPLWYAQGRTDLAQRQIRKVLPHGAAAEPGD
                     VVLLDALVLQRIAADMALDRGDLARALAWIEANDRWLEWSGAVLGRSENRLSWARYCR
                     RAGDLLLAARHVKGAIEAASNPHQPLALIAARRMHGELQTGSGEYESAEHDLTEALML
                     SEACAAPYERALTLIARAELKQAVGAQIDARTELIEARALIMQLHAQPALNRIDALLS
                     RLGSPRDVAPAGLTPREIEVLRLVAKGMTDAEVANRLYISPRTVSQHLRSVYGKLDVS
                     SRSAATRFAIEHNLA"
     gene            complement(5038..5259)
                     /locus_tag="VHR64_13755"
     CDS             complement(5038..5259)
                     /locus_tag="VHR64_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303946.1"
                     /translation="MEIEATVEGMQRIAIHGSPYVRVFYSLDDDRETIHQAQLPDEAV
                     DANIRVGDRVRLTMLLRTVMEVRRLPVSG"
     gene            complement(5302..6036)
                     /locus_tag="VHR64_13760"
     CDS             complement(5302..6036)
                     /locus_tag="VHR64_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303947.1"
                     /translation="MRSERDKWQVPESDPAGKVPQPERGTGSMNLPAPGTGAEPVQSA
                     ERAPLIYCGQCGALNPATNYFCAACGATLVDAFHATEGLRVYDRPDTASRMIEIIPAG
                     SELDLIDDPDAAPDFLRIKLQYGRLGYVRVGDIDALTAARVEPVDPLGTPDINMNARG
                     CVTQSGALLALALLLALTILSLVYISQSSVEEQGIVALVACVSLGPLFLITIAFFIYA
                     RSRDERLAEEAEELGEQTSRRAAERL"
     gene            6274..7314
                     /locus_tag="VHR64_13765"
     CDS             6274..7314
                     /locus_tag="VHR64_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX3303948.1"
                     /translation="MTTSSQGHINERAEQSPADLPIPTLADIFAAKQIVDRYLKPTPL
                     LHSIALSEQLGFDLYLKCENLQPVGAFKVRGGIYLMSQLQPEYRAAGVVTASTGNHGQ
                     SIAYAAREFGVQATIYMPEQANPVKVASMRRLGAEIVFHGKDFDDSRQQAEIDAAATG
                     RYFIQSANDDRLVTGVGTYTLEIMQDLPRLDVLFVPVGAGSGVCGGLIAGKGVNPSLT
                     VYGVQAEGAAVVADSWRDRALHQYASADTFAEGLATRVAFEYPARIMWDHIDGFVLVS
                     DDEMRAAMLTLMESAWIVAEGAGAAATAAAFKKRAELQGKTVCCVVSGGNVPLAGLRD
                     ALAKGMAGGAIR"
     gene            7298..9490
                     /locus_tag="VHR64_13770"
     CDS             7298..9490
                     /locus_tag="VHR64_13770"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3303949.1"
                     /translation="MARFVDWLSGRGRPDGHSSVILQFAREVVERRRSMQGALNEVRH
                     PAVLDSLSDDDFRALDDIIAENAEQHQEFAIVLARLAHAAARAKGFDRQIVDAAIRLD
                     SLLPLDDPSRERDQLLRDAYVVAQKAGYARGGRITLARLADRALDNDDLERARKLFSQ
                     QLEIGDESTDSRVEVDSAISCGDILRREGDILGAQAMYRRASRSGMRLDYHHGVAESL
                     VRQIDLVDAGTSLETVAQMQRQALDAAERTSDLGLQSRILLMHAETLTRLGRTEDVAP
                     VLADGVEIARDIGDLSLESRCLQALVDVEDQLGREDAVADHLTDWLELEERLGNRTAA
                     GSIASRLGMTLLRINDPDHAVEAFTRARSMATTLRDPEMEQQSFGGLGVAYAQLNEPS
                     KAIDNLMKALEVARQSADIEGEARWLATIAQTLMQYGQYTESIRAVNDALAITRRMPD
                     ESLQADLLTMLGQLYLKQDQAPRARESFTRALEIYKRTGPRAEEMRVLVALGQLASAG
                     RQSALAISQYEQALAIANETGDRMAQARLHGRVGQLLQSQRDTIGALEHYRRAVDAAE
                     AVDHRGLMERSLMALATAQHTINDPAAMSTYRRVLTMVQESGHPDREAMIHYNMGLLD
                     VADGNNNEGLKHLYRASDILADADMLDTELGDAVEDAIVASGGHTLARVTHASRDQYE
                     DDYDEDYDEYEDERDDERWEPGETWREYGDQLDYPPDEIYGESTLPPQ"
     gene            9613..10401
                     /locus_tag="VHR64_13775"
     CDS             9613..10401
                     /locus_tag="VHR64_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303950.1"
                     /translation="MATELQSRDNVLLITAPTPDPRAGLILNTYLDVDRLIGFAQPTI
                     WSDRGIEIARRALDSAHHALIYGRELADLSAAGRLQRALTAANDAVIDANHARSGNDH
                     ERRVGVGMGLCLRSGRTAMLALVPPVQAILFQGAGPVWNPRRESWVGDDPGLPGSPLG
                     WAPEVSPTLVSTVIEHADEILLTTTAVAARLAERDELPKSAAAISDEIATLTYGVDPL
                     EIVALSTRFEPATVSGNLRAITQQALGLVDRRARAVWTALRTPA"
     gene            complement(10495..>10748)
                     /locus_tag="VHR64_13780"
     CDS             complement(10495..>10748)
                     /locus_tag="VHR64_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX3303951.1"
                     /translation="RSEMAYVITQPCIGTKDASCVEVCPVDCIHSTDEADQYFINPDE
                     CIDCGVCAEVCPVEAIFFEDDVPEQWANFIPLNKAFFDK"
BASE COUNT         1978 a         3488 c         3460 g         1822 t
ORIGIN      
        1 ggccggatcg atatccccgg cgcgaatgag gcagatatga tcgcctcgct gaagcgcctc
       61 gccgccctcc cggacgatgt cgtcgtctac ccgggccacg gcgcgccgac aaggataggc
      121 aacgagcggg gatggctccc cacctagtag tacgtcgtga gacgtgagac gtgagcagga
      181 aacaaaagcc ggcggagcga atccgccggc ttttgcagta tcaggaccct actgggtcaa
      241 cgagagactc ttaaccgcta accactaacc accaattact atcccctcac cttatgttct
      301 gattaacgtg gaagacgttg tctgggtcgt acgtgcgctt gacgtcgagg agccgggcgt
      361 agttgtcccg gtaggtggcc cgaatccggt cctgaccctc gtccatcagg aagttgaggt
      421 aggcgccgcc ggcagactcg ttgtgaaggg ccgaccagta ggcgcggacc cagtcccgcc
      481 gagccggcat attggctgga tcggtgtcga tgccggcgat gatgtgcgcg aacttgacgt
      541 cgcgatggct gaatgccgta gcgtccttcg cgacatcctg aacggcgccg tcgagcgggt
      601 agaggtgcat gagcgacatg aagttcggca cctgcgcccc gtgctcggca tggatccgga
      661 tcgcctcgtc ggttagctcg tagtcgaagt cggccttcca gtagtgctgc aatccggccg
      721 gcaagagtgc gtcgaagagg ctgttcagcg ccggataggg gatcgggccc gccagatcca
      781 gcgcgaccgg cccggcctca cgaatcgggc ggaccgcctc ttcggccgac tccatcggac
      841 cggtgtagca gatcacgatc gcgcaggcgg tgtgaccgtg cagcgcctcc ggaatgaacg
      901 gtgcgggcgg cgcgacgtgg tacgcgaaga aggcactcat ctcgcgcggc gcgctcgcga
      961 cgaaatcacg atagaagcgg agtacctgcg cgctggcatc gaccgggaag aagatcgggc
     1021 cgccgtaaac catgccgacc ggatgcatcc ggaactcaaa tgacgtcacg acgccgaagt
     1081 tgccaccgcc gccgcgaatt gcccagaaca gatccgggtt ctcgtcagca ctggcggtca
     1141 ggcggtcgcc gtccgcggtg acgacgtcgg cggagatcag attgtcggca gagagcccgt
     1201 agcgtcgggt caggtaaccg aagccgccac cgagcgtgag tccgccgatc cccgtcgtcg
     1261 aaagcacgcc gcctggggtg gccaagccat atgcgtgacc agcgtggtcc atgtctgccc
     1321 aggtcgcgcc tccgccggct cgggcggtgc tcgttttcgg atcgacccgg atgttgcgca
     1381 tgagtgaaag gtcgaggacg acgccaccat cgttagtgcc gaagcccgcg acgttgtgac
     1441 ctccacctcg gacggaaaga tcgagattgc gcaccttcgc gaagcggacg gtggagatga
     1501 catcgctgtc atcggctgcg cgcacgatca ccgcggggta gcggtcgatc atgccgttgt
     1561 aggtcttgcg cgcctcgtca tacgtcggtg accctggctc gatgatctgg ccgcgaatca
     1621 tggggacgag ggcttccacg tcgatctcga ccgtgttcgg gctttgaaca gtagctgcca
     1681 tgtcgtttgg aatcctttcg gaactgaaat cggatgctgg catcgatgcc aactccttca
     1741 agctacgtcc ggaaggcgcc gcgcacatcg acagaactgc gcaaccggca aaggcaattt
     1801 ccgaggggtg tgtctacgta gaactgcgta gtccgggctg cgaaggagat cagccctggt
     1861 ggccgcgcta cgtctgctcc cggtaggtca gttcgaatga cccctaggcg aggttgtgtt
     1921 cgatggcgaa gcgggttgcc gctgagcgag aggagacgtc gagcttgccg tacaccgatc
     1981 gcaggtgctg gctgacagtg cggggactga tgtagagccg gttggcgacc tctgcgtcgg
     2041 tcattccctt ggcgacgagg cgcagcactt cgatttcgcg cggagtaaga cccgcgggcg
     2101 caacgtctcg tggtgacccc aatcgggata gcagcgcatc gatccgattc agggccggtt
     2161 gcgcgtgaag ttgcatgatg agtgcacgcg cctcgatcaa ttcggtcctg gcgtcaattt
     2221 gcgcgccaac tgcctgcttc agctcagccc gcgcgatcag cgtcagggct cgctcgtatg
     2281 gagcggcaca tgcctctgac agcatcagcg cttcggtcag atcgtgctcg gccgactcgt
     2341 attctccgga gcccgtctga agctcaccat gcatgcgacg cgctgcgatc agagcgagcg
     2401 gctgatgagg atttgacgct gcctcgattg ctcccttgac gtgacgtgca gcgagaagga
     2461 gatctcctgc ccgtcggcag tatcgtgccc agctcagccg gttctcggac ctcccgagaa
     2521 cggcgccgct ccactccagc cagcgatcgt tggcctcgat ccaggccagg gctctggcga
     2581 gatcgccgcg atcgagggcc atatccgcgg cgatacgctg caacaccagc gcatcgagca
     2641 ggaccacgtc gcctggctcg gctgccgcgc catgcggcaa caccttgcgg atctgacgtt
     2701 gggcgagatc cgtcctgccc tgtgcgtacc aaagcggcgt catggcattg gtaatctcgc
     2761 gccggagcac gtagttgccg tctgcctcaa cctcgctggc gatcgcgtcc gcctcgtccc
     2821 atcgaccctc gagaaagagc aggccgagcc gagcgcgccg cgacgagcga tcggacggaa
     2881 atgccccggc ggcgaggttc agcgatattt cggcatccat cgctaaccgg gcccgacccg
     2941 ccacgtcggt cgcgaaatag ggcagcacta cgtcgcgcag ctcactcaac tgggtgaatc
     3001 cgatgaccgc gtggtgctcg atcaggtcgt agatctgccg ggctcgggag aatgcctgac
     3061 gggcctcatc gggccgaccg agtgccgcat ttgcgagccc gagcccgaac caggagtgac
     3121 ccgtggccga gatgaccagc tcgccggcgg gtatcccggc ggtgagcgcg atgaacttct
     3181 ccgccatcgc cttcgcccgg gccaggtgtc cggccgctgc gaggaaccac ggatggccac
     3241 cgcgccgatg atgcgtccca gcggcgatca tgatgcgggc ggacggctca atgtccggga
     3301 gatcggccag ctcgcgggga gggagggcat cgaccatcca ggtcgctcgc tcccagctct
     3361 ccatctgttg gtcggacgtc ataccttcga gctcgacgat tccgctttcc atttcgacca
     3421 gccccggacc gaaatcgtcc gagaagcagc gcaacaatcc gcgagagtac ttggcatcgg
     3481 ccgccagcac gcgattcccg gcctggttcg cagcgcgctc ggcggcggcc agattcacga
     3541 tgccgtcaga tatctcggaa taacgctgca aacggcccga tcggtagagg agtcggctcc
     3601 ggcgcgcctc catcccgggg acgttctcga tcaaggcggc ggccgcaacg aagcggtcct
     3661 ttgccatcag ccaggcgtat gcgcgctgtg cccgctctcc ggcacgcacg taccactccc
     3721 atgcatgggg atcgccggcg cgctcgaagt ggaacgcgac ctcgtccgga ctgaccctcg
     3781 gtagcgccgc cagggcgtcg ccgacgcgct gatgccacag ccggcgacgc ggcggcatga
     3841 cggcctcgta aagtgcctcg cgggtcagcg catggacgaa tcgcacccgg gtgccatccg
     3901 gagccgcgtt gaggaggtgc gcatcgatcg ctcgctcgac gatcgtcagg agctgctcct
     3961 cgtccaaccc caggacgcgc tcccacaaat cgagtgggat gtcgtgaccg atcacagccg
     4021 caacactcaa tggcccgcgc gtttcatccc ccagacgcga tatccgcgcg tcaatcacct
     4081 gccgcagcag cgacggcacc acgatccgat caagctcgcc cagactccac cgctcgccat
     4141 ccggtctcag gaatccctcc tccgaaaggg cccgaagcaa ctcaacgaca aagaacgggt
     4201 tcccttcggc atgccgctcg agatatgcga cgagccgctc cccctcctcc tccgacatgg
     4261 cgtagttccc atgtaccagc gatcgcaaat ccggcgcggc cagcggacgc aggtccagac
     4321 gccggccgcc ggtttcgcgg agcagtgacg gcagctggtg gtagagcggc tgagtgcgcg
     4381 tcagctcgtc actgcgatag gtgaccagca ctagtatggg ttgcgcagca accgccgcac
     4441 cgagcgatct caggagctcg atgctggccg gatccgccca gtgcagatcc tccaaaacga
     4501 ccagcaccgg ctggaccgac gccagcgcgg cgaagaagga ccggacgtcg gcgaagagtg
     4561 cggcctggct ctcgatctcg tcgatacggc cggtggcgag cgcctcggga agtaacggca
     4621 tgccgtttgc cggccggtag gtcgccgcca gccccagcca gggtccatat ggaggagtga
     4681 cggtcaaatc gtaacagtga ccgatcaaga cacaaacgcc ggccgtggtc gcctcgtaaa
     4741 ccagctcccg ggccaacgac gtcttgccga tcccggcctc gccgctgagc acgatcaggc
     4801 cgccgcggcc atctcgggcg gcagccagat cggcgcggag cagttcctgt tcgtcgcctc
     4861 gacccacgag cgaaggcggt tccagggatg aacccgagtt tcggatgtgc gtatgcgaca
     4921 tggcaccgag cagcgcgcgc cggttttgcg agcaccggag aatgcaggta tcgaccgata
     4981 cattgatctt atcgtatgag attcggtagc ggatccaacc ttcggcggag gaaccgatca
     5041 gccggacacg gggagccgtc gaacttccat aacggtgcgc aagagcatgg tcaggcggac
     5101 acgatcgccg acccggatat tggcatcgac cgcttcatca ggcagctgcg cctggtggat
     5161 cgtctcgcgg tcgtcatcga gtgagtagaa gacgcggaca tacggcgacc catgaatcgc
     5221 gatccgctgc atcccctcga ctgttgcctc gatctccatc gatcagactc cggataggtg
     5281 cggacgcaat gcaatgcgtc actacaaccg ctcggctgct cgcctgctcg tctgctcgcc
     5341 taattcctcg gcttcctctg ccaagcgctc gtcccggctg cgcgcataga tgaagaaggc
     5401 aatcgtgatc aaaaaaagtg gccccagcga gacacaggca acgagagcga cgatgccctg
     5461 ctcctcgacc gagctctgcg aaatatagac aagcgagagg atcgtcagcg ccagcagcag
     5521 ggcgagcgca agcaacgcgc ccgactgggt cacgcagccg cgcgcattca tgttgatgtc
     5581 aggcgtgcca agcggatcga ccggctcgac ccgtgccgcc gtcagcgcgt cgatgtcgcc
     5641 cacacggacg tatccaaggc ggccgtactg cagcttgatg cgcaggaagt cgggcgccgc
     5701 atccgggtcg tcgatgagat ccaactcgga accggccggg atgatctcga tcatccgcga
     5761 ggcggtatcc ggccggtcgt agacgcgcag tccctcggtg gcgtggaagg cgtcgaccag
     5821 ggtcgccccg cacgccgcgc aaaagtagtt cgtggccggg ttgagtgcgc cgcactggcc
     5881 gcagtagatc agcggcgccc gctcggccga ctgaaccggc tccgcaccgg ttcccggcgc
     5941 gggcaagttc atggaaccgg taccgcgctc cggctgcggg actttcccgg ccggatccga
     6001 ctccgggacc tgccacttgt ctcgttcgct ccgcacaatg ctccccagtg ctgcttccgc
     6061 cgcgccactt gcgcctgtat aacaaagatt gcgccaggca aggacgtcga aaccacgcga
     6121 cggggtaggg ctgcgcaact ggttgcgtgt aattttgcat ggtcggagta ccgtgctaga
     6181 ctactcaaaa ctattcaatt ctgggtagtt gacccgatgc caaggcggtc gggatagcgt
     6241 cgttctgtgc tgaatctgac tggttggagc cgcatgacaa cctcctcgca aggtcatatc
     6301 aacgagcgcg cagaacaatc tcccgccgat ctcccgattc ctacgctcgc cgatatcttc
     6361 gccgccaagc agatcgtcga tcgctacctt aagccgactc cgctgctgca ctcgatcgcg
     6421 ctgagcgagc agctcggctt tgatctctac ctcaagtgcg agaacctgca gccggtcggc
     6481 gccttcaagg tccgcggcgg tatctacctg atgagccagc ttcagccgga ataccgagcg
     6541 gccggtgtgg tgaccgcgtc gaccggcaac catggccagt cgatcgcata cgcggcgcgc
     6601 gagttcggtg tccaggcgac gatctacatg ccagagcagg ccaacccggt caaggtcgcc
     6661 tcgatgcggc gactgggcgc ggagatcgtc ttccacggca aggacttcga cgatagccgg
     6721 caacaggccg agatcgacgc cgctgccact ggccgctact tcatccaatc ggcaaacgac
     6781 gaccggctcg taaccggcgt cggcacctat accctcgaaa tcatgcagga ccttccccgc
     6841 ctcgatgtcc tgtttgtacc cgttggcgcc ggaagcggtg tgtgtggcgg attgattgcc
     6901 gggaaagggg tcaatccatc gctcaccgtc tacggtgtcc aggcggaagg agcggcggtg
     6961 gtcgcggatt cctggcggga tcgcgcgttg caccagtacg catccgccga cacgttcgcg
     7021 gaagggttgg ctacccgggt cgcgttcgag tatccggcac ggatcatgtg ggaccacatc
     7081 gatggcttcg tgctcgtcag cgacgacgag atgcgcgccg cgatgctgac tctcatggaa
     7141 tcggcctgga tcgttgcgga aggcgcgggt gcggctgcca cggcggccgc attcaagaag
     7201 cgcgccgagt tgcaggggaa gactgtctgt tgcgtcgtca gcggtggtaa tgtcccactg
     7261 gcaggactgc gcgacgcatt ggcgaagggg atggccggtg gcgcgattcg ttgattggct
     7321 gagcgggcgg gggcgtccag acgggcacag cagcgtgatc ctgcagtttg cccgcgaggt
     7381 cgtcgaacgc cggagatcga tgcagggagc cctgaacgag gtacgccatc cggcggttct
     7441 cgactcgctc tcggacgacg atttccgcgc tctcgatgac atcatcgccg agaacgccga
     7501 gcagcatcag gagttcgcga tcgtcctcgc ccggcttgcg cacgccgcgg cgcgagccaa
     7561 gggcttcgac cgccaaatcg tcgacgcggc aattcgcctc gatagcctgc tcccgctcga
     7621 cgacccgagc cgggaacgcg atcagctctt gcgtgatgcc tatgtggtcg cccagaaagc
     7681 cggctacgcc cgcggcgggc ggataaccct tgctcgtttg gccgaccgcg ccctcgacaa
     7741 cgatgacctc gagcgggccc gcaagctctt cagccagcaa ctcgagatcg gcgacgagtc
     7801 gaccgactcc cgggttgagg tcgattccgc gatctcctgc ggcgacatcc tccgccgcga
     7861 gggcgatatc ctcggcgcgc aggcgatgta ccgccgggcc agccggtccg ggatgcggct
     7921 cgattaccac catggcgtgg ctgagtcgct ggtccgccag atcgatctgg tcgacgcagg
     7981 cacaagcctc gagacagtcg cgcagatgca gcggcaggcg ctggatgcgg ccgagcgcac
     8041 ctccgatctc ggactgcagt cccgcatcct tctgatgcac gcggagacgc tgactcgact
     8101 cgggcggacc gaggatgtcg cgccggtact ggccgatggt gtcgagatcg cacgggatat
     8161 cggcgacctc tcgctcgaaa gtcgctgcct gcaggcgctg gtcgacgtcg aggatcagct
     8221 cggccgcgag gacgcggtcg cagatcacct gaccgattgg ctggagctgg aagagcgcct
     8281 cggtaaccgg acggcggcag gctcaattgc cagccgattg ggcatgacgt tgttgcggat
     8341 caacgacccg gaccatgccg tcgaggcctt tacacgggca cgatcgatgg cgaccacgct
     8401 ccgcgatccg gagatggagc agcagtcgtt cggcggtctc ggtgtcgcct acgcgcagct
     8461 caacgagccg tccaaggcca tcgacaacct gatgaaggcg ctcgaggttg cccggcagtc
     8521 ggccgatatc gagggcgagg cccgctggct ggcgacaatc gcccagacgc tgatgcagta
     8581 cgggcagtac accgagtcga tccgcgcggt caatgatgcg ctcgcgatca cgcgccggat
     8641 gccggacgaa tcgctccagg ccgatctcct gaccatgctt ggccagctct acctgaagca
     8701 ggatcaggcg ccgcgcgccc gcgagtcatt tacccgcgcg ctcgagatct acaagcggac
     8761 cggaccgcgt gccgaggaga tgcgcgtgct cgtcgcgctc ggccagctcg cttcggctgg
     8821 ccggcagagt gcactcgcca tctcgcagta cgagcaggcg ttggcaatcg caaacgagac
     8881 aggcgatcgg atggcccagg cccgcctgca tggtcgcgtc gggcaactcc tgcaatccca
     8941 gcgagatacg atcggcgcgc tcgagcacta ccggcgcgcc gtcgacgcgg ccgaggcggt
     9001 cgatcatcgc ggattgatgg agcggagcct gatggcgctc gcgaccgccc agcacaccat
     9061 caacgaccct gcggcgatga gcacgtatcg ccgcgtgctg accatggtcc aggaatcagg
     9121 gcacccggat cgcgaggcga tgatccacta caacatgggc ctgctcgacg tcgcggacgg
     9181 caacaacaat gagggcctga agcacctcta ccgcgcatcg gacatcctgg ccgacgccga
     9241 catgctcgac accgagctcg gcgatgcggt ggaggatgcg atcgtcgcct cgggtggtca
     9301 cacgctggca cgggtaacgc acgcctcgcg cgatcagtac gaggacgact acgacgagga
     9361 ttacgacgaa tacgaagacg agcgtgacga cgagcgctgg gagcccggcg aaacctggcg
     9421 cgaatacggc gatcaactcg actacccgcc ggatgagatc tacggcgaat cgaccctccc
     9481 accgcaataa ttgtcccgac aattgactgt acgtacactc ggcggtacgc ttgactcgaa
     9541 gtcacgcctg cccgtcgttg acggttcacg ttcgccgggg cgggcaccag cctacactcc
     9601 ggaggttcgc cggtggcaac cgaattgcag tcgcgggaca acgtcctgct catcaccgca
     9661 ccgacgcctg atccccgtgc cgggctaatt ctcaatacgt acctcgatgt ggaccggctg
     9721 atcgggtttg cgcagccgac gatctggtcg gaccgcggca ttgagattgc ccggcgcgcg
     9781 ctcgattcgg cgcaccatgc cctgatctac ggccgtgagc tggcagacct gtcggcagct
     9841 ggtcgactgc agcgcgccct gactgccgcc aacgacgcgg tgatcgacgc gaaccacgcc
     9901 cgcagcggaa atgaccacga gcggcgggtc ggagtcggca tggggctttg cctgcgctcg
     9961 ggtcgaaccg cgatgctggc gctggtgccg ccggtgcagg cgatcctgtt ccagggtgcc
    10021 ggcccggttt ggaacccgcg gcgcgaatcg tgggtagggg atgatccggg cttgccgggg
    10081 tcgccgctcg gctgggcgcc agaggtatca ccgacgctgg tctcaacagt gatcgagcac
    10141 gcggacgaga tcctgctgac gacgactgcc gtcgcggcgc gcctggcgga gcgggatgag
    10201 ctgccaaaat cggccgcagc catatccgac gagatcgcta ccctcacgta tggcgttgat
    10261 ccgctcgaga ttgtcgcact ctcgacccga ttcgagccgg cgacggtcag cggcaacctc
    10321 cgtgcgatca cccagcaggc actcggcctc gtcgaccgcc gcgcccgcgc cgtctggacc
    10381 gccctccgca ccccggcctg actccgagcc gcgctacgtc ttgtcggcat ggtccaatgg
    10441 aacagggccg ggcggagttt ccgcccggcc ctgtttctgc ccgtttgaac ctggctactt
    10501 gtcgaagaag gccttgttca gcgggataaa gtttgcccac tgctccggca catcatcttc
    10561 gaagaagatg gcctcaaccg gacaaacctc ggcgcagacg ccgcagtcga tgcattcatc
    10621 cggattgatg aagtactggt cggcttcatc ggtcgaatgg atgcagtcga ccggacagac
    10681 ctcgacgcac gaggcgtcct tcgtgccaat gcacggctgc gtgatgacgt atgccatctc
    10741 tgacctcc
//
LOCUS       DATAOZ010000545         6822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2583486, whole genome shotgun sequence.
ACCESSION   DATAOZ010000545 DATAOZ010000000
VERSION     DATAOZ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2583486"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VHR64_13785"
     CDS             <1..1494
                     /locus_tag="VHR64_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303952.1"
                     /translation="RWTAILATAAIVLLALAASIRGLSPAFLGDDGKAPAVSAPNGQS
                     GVTQRMLLDIALTAEESAFSGQYGTQMGYFSSPPGSHDVWPSKSDGSPEARVVYIMSG
                     SARIKSDQPLRVVRGSGQGTVEQMTSGTWFVLDPGDSLLRAPGVTLELENPGTQPYFV
                     LDWTIGDVNRIVESEPSSWTMHNYTASKVGVQPNDRVRFTLRLVTLAKDAVLSAPTGA
                     LSQQYLDITSDQRYVIGQDSDYSITNRSSTQAELYVVSLEPVTQVTTDAGTRSEPDET
                     LLDVVLPDEQLQTPGRFNSIMGYYSIPAGSQDSWSGPTDTAPEPLVWHVLSGTYSVRA
                     DQDVQVTRAGASGKTSVVAAGNESTLGPGDTLVRPAGALMELANDGTEPVLLVVWTLG
                     DINVQGPSLPSSWDSLPCYPGETSGTAPSGPVRLKLRLVTVTPGSTFSAPAGAFVQQF
                     LDLTSQAPNLGQKSDYSLINQGTEPVTLYVVSLEPASPDEATPVSST"
     gene            1658..2551
                     /locus_tag="VHR64_13790"
     CDS             1658..2551
                     /locus_tag="VHR64_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX3303953.1"
                     /translation="MNISRIGVALLISLALTWVPVRADAALTCVVAGAFYAPTIVGSG
                     VITGTPGMDVIQGSDGDDTINALGGNDKICAGDGNDTIDGGDGPDLIDSGPGVDVIHG
                     GADGDAIFAGSGDDQVFGGIGDDNLFGKDGNDRIWGEEGDDQLWGGDGDDLIDGSAGV
                     DILLGEAGDDTLFVGSRLEAATCHLRGFGPSFDDYGLGGAGDDRIFGGSGNDSLDGDD
                     GDDVIWGGNGADLIAGGRGDDHIYGQAGQDNLFGHDGNDLIMSGPGPDQMNGGPGENR
                     LIGDRAVAGVVLMDVCTEAIL"
     gene            complement(2566..3801)
                     /locus_tag="VHR64_13795"
     CDS             complement(2566..3801)
                     /locus_tag="VHR64_13795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3303954.1"
                     /translation="MALAPPAWARLAGTTDPVAQLLVETRALRGFADGLVSVLLAGYL
                     VDLGFGARQVGLITTMTLVGSAAATLSLGLVAHRWSRHALLVIAAIVMALTGLAFLAV
                     QSFWVLLPIAFVGTLNPSSGDVSVFLPLEQAEIAHVIPATERTTVFARFSLAANLAAA
                     FGSLTAGAIAAVVASQALDPERAGQAGFLVYIGIGLFVFSRYRNLPADPTATIEGAPR
                     HGLHKSRNVVMKMAAVFSLDSLGGGFVIQTMLILWLHERWGMSAASAGLLFSVASLLA
                     AFSMLAAPRLANRIGLINTMVFTHLPANIFLMITPFMPTLPLAITTLLLRWSLSSMDV
                     PARTAYVMSVVEPDERAAAASVTNVPRSLASAVSPAIAGQLLSMTAFGWPLLVGGGMK
                     AAYDLILLKMFRDIPPDRG"
     gene            complement(3804..4646)
                     /gene="nudC"
                     /locus_tag="VHR64_13800"
     CDS             complement(3804..4646)
                     /gene="nudC"
                     /locus_tag="VHR64_13800"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX3303955.1"
                     /translation="MAYRVFEPGVDPNAILGGDAVCFVFRKGDLLVGPSAIGDYLPIY
                     DELSQISHLILRSNYMGELDGRPTFAVEVDESAPDPLPFAFEQLRGTFGTLDEGAWLI
                     AGRASMIVDWDRSHQICGQCGHRTHYLDTERAKRCPNCGLVAYPRVSPAVIVLVERGE
                     DVLLARGIRQSSGMYALIAGFVEAGESLEEAVHREIQEEVGIEVDGVRYFGSQPWPFP
                     HSLMVGYTAQYKAGDIRIKPDEIADAQFFAPHALPPIPPRLSIARKLIDYYAEKHGIE
                     LNVP"
     gene            complement(4694..5167)
                     /locus_tag="VHR64_13805"
     CDS             complement(4694..5167)
                     /locus_tag="VHR64_13805"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX3303956.1"
                     /translation="MTSLPVSSPMLGWHRRPELWSVVLSAVGVALAGYLTIVHFRESL
                     LVCSGISDCETVQTSQYAEILGIPVALLGLLCFVALFGLAIVRIFQPERADIITMIMF
                     VMVVGSIGFYIYLTYLELFVIDAICQWCVASSLVMIGMLVTESILLRRVLSIPEE"
     gene            complement(5145..6002)
                     /locus_tag="VHR64_13810"
     CDS             complement(5145..6002)
                     /locus_tag="VHR64_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX3303957.1"
                     /translation="MAKSKRQRQRASQQTAARPTTDTTQPRRTGGPGPQRQSITAAAQ
                     QAQRRKEMMKWLGTGLLLAVFAVVAVIAIQQSRDSGGSGSNTDVATVVAAQPIPEGVQ
                     QNGNILGDPNAPVLVVEYGDYQCPFCKKFAIEDYPKLIQDYIQTGKVRLEFRQYPIIG
                     RNSDGSIDQQGESFHAAEAAVCAQDQGYFWQMHDLLYENSVGEFKGSFTIDRLKKIAA
                     LIPGMDQATFGTCLEGSSHTQTILASVSEGTSSGINSTPSFIVGDQKISGADYNGLKK
                     LIDDQLAGK"
     gene            complement(6095..>6822)
                     /locus_tag="VHR64_13815"
     CDS             complement(6095..>6822)
                     /locus_tag="VHR64_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX3303958.1"
                     /translation="TTGNTFEAHTAGMSVAGDWGMTLTVSQVGAFQWSAELTHTAAER
                     KPGARSISIPTWTINGAGLIGFLAAMAGVIGLVIGWRSDNRSTRREGYGLGSVAIAAG
                     LLLLTQGRSDVASAGIPLDTPNPVPLTDQAVEVGKNAFQANCIACHGVGAAGDGPAAQ
                     GMVPPPANLLEGHALYHADAEFFNWIRNGKPGTAMPGFSDALTDEEIWSTIHYIRELQ
                     QEHAESINTTPAATPTEGATPIP"
BASE COUNT         1289 a         2163 c         2175 g         1195 t
ORIGIN      
        1 cgttggaccg ccatcctggc gaccgccgca atcgtcctgc tcgccctggc agcatcgatc
       61 cgcggcctga gtcccgcttt cctcggcgac gacggaaagg ccccagcggt ttcggcgcca
      121 aatggccaat ctggcgtaac gcagcgaatg ctgctggaca tcgcgctgac cgcagaggaa
      181 tcagcattca gcggtcaata tggcacgcaa atggggtatt tcagctcgcc gccgggttct
      241 cacgacgtct ggccaagcaa aagcgacgga tctccagagg cccgtgtggt ctacattatg
      301 agcggctctg cccggattaa atccgatcaa ccactgcgcg tggttcgtgg aagcggtcag
      361 ggtaccgttg agcagatgac atcagggacc tggttcgtcc ttgacccagg cgattcgctc
      421 ttgcgcgcgc caggcgtgac gctcgagttg gaaaatccag ggacccaacc atacttcgta
      481 ctcgactgga caatcgggga cgtcaatcgc attgtcgagt ctgaaccgtc aagctggaca
      541 atgcacaact acaccgcgag caaggtagga gttcaaccga acgatcgggt gcggttcaca
      601 ttgcgcctcg taactctcgc gaaagacgcg gtcctgtctg ctccaaccgg agcgctatcg
      661 cagcaatacc tcgatatcac gtcagaccaa cgttacgtca tcggacaaga ttccgattat
      721 tcgatcacca atcggagctc cactcaggca gagctgtacg tcgtctcgct cgaaccagtc
      781 acgcaggtga ctactgacgc aggaacgcgg tcagaaccgg acgaaacgct cctcgacgtc
      841 gttttgcccg acgagcaact ccagactccg ggccgtttca actcaatcat ggggtattac
      901 tccattcctg ctggctcgca ggattcctgg tcggggccga ctgacacggc tcccgaaccc
      961 ctggtctggc atgtgctgag tgggacctac tccgtccgcg ccgaccagga tgtgcaggtc
     1021 acacgggctg gcgcgagcgg caagacaagc gtcgtggctg cgggcaatga gtcgacactg
     1081 ggacccggcg acaccctggt ccgtccagcc ggggctctca tggagctcgc aaacgacgga
     1141 actgagccgg tcctcctggt tgtctggacg ttgggcgaca tcaacgtgca ggggccttcg
     1201 ctgccgtcgt cctgggatag cctgccgtgc tatccgggcg aaacaagcgg gaccgcgcca
     1261 tccggaccgg tccggctgaa gctccggctc gtcacggtca cacctggctc gacgtttagc
     1321 gcacccgcag gcgcatttgt gcagcaattc ctcgacctaa catcgcaagc accaaatctg
     1381 ggccagaaat cggactactc gttgatcaac cagggaaccg agccggtgac cctgtatgtc
     1441 gtcagcctgg aaccggccag tccggacgaa gcgacgcccg tttcatccac gtgatctcct
     1501 gagttctcct ggctgctgag gtaccaagcg gccagtccga actcatcgcg gaacccggcg
     1561 caagccggac tccatccaca acttcacgtt cgcagtctgc gtccccacgg gacgcgagta
     1621 gccctggcat gcccatgcca gcatggaagg tcctgccatg aacatctctc gaatcggcgt
     1681 cgctctcctc atctcgctcg ccttgacttg ggttccagtc cgcgcggacg ccgccctcac
     1741 ctgtgtcgtc gccggagcct tttacgctcc cacgatcgtc ggatccggcg ttatcaccgg
     1801 cacgcccggg atggacgtca tccagggctc ggacggtgac gacacgatca acgcgctcgg
     1861 cggcaacgac aagatttgcg ccggcgatgg caacgacaca atcgacggag gagacgggcc
     1921 ggacttgatc gacagcggcc cgggagtcga cgtcatccac ggcggcgcgg acggtgacgc
     1981 catcttcgcc ggatcaggcg acgatcaggt cttcggtgga atcggtgatg acaacctgtt
     2041 cgggaaggac ggcaacgatc gaatctgggg cgaggagggc gatgaccagc tctggggcgg
     2101 cgatggtgat gatctgatcg acggcagcgc cggagtcgat atcctgctgg gtgaagcagg
     2161 cgacgacacg ctcttcgtag ggtcgcgcct cgaggctgcg acctgccatc tccgcggctt
     2221 cgggccaagt ttcgacgatt atggactggg cggtgctggt gatgaccgca tattcggtgg
     2281 atccggaaac gattcactcg acggcgacga tggcgacgac gtcatctggg gcggcaacgg
     2341 cgcagacctt atcgcaggcg gccggggtga cgatcacatc tacggccagg cgggccagga
     2401 caacctcttc ggccacgacg gcaacgacct gatcatgagc ggacccgggc cggaccagat
     2461 gaatggtggg ccgggcgaaa atcgcctgat cggagatcgc gcggtcgccg gagtcgtcct
     2521 gatggacgtg tgtactgagg caatcttgtg aacacgtatt gcgggtcagc cgcggtccgg
     2581 cgggatatcg cggaacatct tgagcagaat caggtcatag gcagctttca tgccgccgcc
     2641 gaccagcaat ggccacccga acgcggtcat actcagcagt tggccggcaa tggccgggct
     2701 gaccgcgctg gccaagctcc gtggtacgtt cgtgacgctc gccgccgcgg cccgctcatc
     2761 cggctcaacc accgacatga cgtaggccgt ccgcgccggc acgtccatgc tcgaaagcga
     2821 ccagcgcagg agcaaggtcg tgatcgccag cggcagcgtc ggcatgaacg gcgtgatcat
     2881 caggaaaatg ttggccggga ggtgcgtgaa gaccatcgtg ttgatcaggc cgatccggtt
     2941 cgccaggcgg ggtgcggcca gcatcgaaaa cgctgccagc aggctcgcca ccgagaagag
     3001 cagaccggcc gaggccgccg acatgcccca ccgctcgtgt agccagagaa tcagcatcgt
     3061 ttgaatgacg aagccgccgc cgagcgagtc caggctgaac acggccgcca tcttcattac
     3121 gacattccgg gatttgtgga gaccgtggcg gggggcgcct tcgatcgtcg cggtcggatc
     3181 agccggcagg ttgcgatacc gcgagaagac gaagagccca atcccgatgt agaccaggaa
     3241 tccggcctgt ccggctcgtt ccggatcgag cgcctgggac gccaccactg ccgcgatcgc
     3301 tccagccgtc aatgagccaa acgcggcggc cagatttgct gccagactga accgggcgaa
     3361 gacggtcgtc cgctccgtcg ctggaatgac atgggcgatc tccgcctgct ccagtggcag
     3421 gaagacgctg acgtcgcccg acgacgggtt gagcgtgccg acgaacgcga tcggcagaag
     3481 cacccagaac gactgcaccg ccaggaaggc gagtcccgtc agcgccatca cgatcgcggc
     3541 gatcaccagc agcgcgtggc gagaccagcg atgcgcgacc agcccgagcg acagggtcgc
     3601 cgcggccgat ccgaccaggg tcatcgtcgt gatgagaccg acttgccgcg ccccaaagcc
     3661 aagatcgacc agatatccgg ccagcaagac gctgaccaat ccgtcggcga agccccgcag
     3721 cgcgcgcgtc tccaccagga gctgcgcgac cggatcggtt gtcccggcga gccgagccca
     3781 cgccggaggg gcgagggcca tggtcacggc acgttcagct cgattccgtg tttctcggcg
     3841 tagtagtcga tcagcttgcg ggcgatgctc agccgcggcg gaatcggcgg gagggcgtgc
     3901 ggggcaaaga actgggcgtc ggcgatctcg tctggcttga ttctgatatc gcccgccttg
     3961 tattgcgcgg tatagccgac catgagcgaa tgcgggaagg gccacggttg gctgccgaag
     4021 taccggacgc catcgacttc gatcccgacc tcttcctgga tctcccggtg gaccgcctct
     4081 tcgagcgatt cgccggcctc gacgaagccg gcgatcagtg catacatgcc ggagctctgg
     4141 cggatacccc gggccaggag cacatcttca ccgcgttcga cgaggacgat cacagccggg
     4201 gagacccggg ggtacgcgac caacccgcag ttgggacagc gcttggctcg ctcggtgtcg
     4261 aggtaatgcg tcctgtgccc gcactgcccg cagatctgat gactacgatc ccagtcgacg
     4321 atcatcgacg cgcgaccggc aatgagccag gcaccctcat cgagcgtgcc gaatgttccc
     4381 ctcagttgct cgaaagcgaa gggcaaggga tccggcgccg actcatcgac ctcgacagcg
     4441 aaggtcgggc gcccgtcgag ctcgcccatg tagttcgagc ggagaatcag gtggctgatc
     4501 tgggagagct catcgtagat cggcaggtag tcgccgatgg ccgatggtcc aaccagcaag
     4561 tcgcccttgc gaaatacgaa gcagacggcg tcgccgccga ggatggcgtt cgggtcgaca
     4621 cccggctcga agacacggta tgccacgcat gattctccct tctgcacggc attgtatgtg
     4681 ccccgcgcgg tcgttactcc tcggggatcg aaagcacgcg acgaagcagg atcgactcgg
     4741 tcactagcat cccgatcatc accagcgacg aggccacaca ccactggcag atggcgtcga
     4801 tcacgaacag ctcgaggtag gtcaggtaga tgtaaaagcc gatcgagccg accaccatca
     4861 cgaacatgat catcgtgatg atgtcggccc gctccggctg gaagatccgg acgatcgcga
     4921 ggccaaaaag ggcaacgaag cagagcaagc caagcagggc gaccgggatg ccgaggatct
     4981 ccgcgtactg gctcgtctgg accgtttcgc agtcgctgat gccggagcag accagcagcg
     5041 attcgcggaa gtggacgatg gtcaggtagc cggcgagcgc aacgccgacg gccgatagca
     5101 ccaccgacca gagttctggc cggcggtgcc accccagcat cgggctactt accggcaagc
     5161 tggtcatcga tgagcttctt gaggccgttg taatccgcgc cgctgatctt ctgatcgccg
     5221 acgatgaagc tcggcgtgct gttgattccg ctggacgtgc cctccgagac ggacgccagg
     5281 atcgtttgcg tgtgcgaact gccctcaaga caggtgccga acgttgcctg atccatcccc
     5341 gggatcagcg cggcgatctt cttgagtcga tcgatcgtga aggagccctt gaactctccc
     5401 accgagttct cataaagcag gtcgtgcatt tgccagaaat agccctgatc ctgcgcgcag
     5461 acagccgcct cggccgcatg gaacgactcg ccctgctgat cgatcgagcc gtccgagttg
     5521 cgcccgatga tcggatactg gcggaactcc agccgcacct tgccggtctg gatgtagtcc
     5581 tggatcagct tcggatagtc ctcgatcgcg aacttcttgc agaacgggca ctggtaatcg
     5641 ccgtactcaa cgaccaggac cggtgcgttc ggatcgccga ggatgttgcc attctgctgg
     5701 acgccctccg ggatcggctg ggcggccacc acggtggcga cgtcggtatt ggagccggag
     5761 ccgccgctat cacgactttg ctggatcgcg atcaccgcga ctacggcgaa gacggccagg
     5821 agcagcccgg tgccgagcca cttcatcatc tccttacggc gctgggcctg ttgcgcggcc
     5881 gcggtgatcg attgccgctg cgggccgggt ccgccggtgc gccggggttg ggtcgtgtcg
     5941 gttgtggggc gggcggccgt ttgctggctg gctcgttgtc gttgtcgttt gctcttcgcc
     6001 atcgtgcgtt ggattctccc gggaatgatg acgtcatttc gatgccgcgt gcttcagtgc
     6061 tggatcggtc gggcggctcc ggtgatcacg ttcactaggg gataggcgtc gctccttcgg
     6121 tcggcgttgc ggccggtgtc gtattgatcg attccgcgtg ctcctgttgc agctcgcgga
     6181 tgtagtgaat cgtgctccag atctcctcgt cggtgagcgc atccgagaaa cccggcatgg
     6241 ccgtccccgg cttgccgttg cgaatccagt tgaagaattc ggcgtcggcg tgatagagcg
     6301 cgtgtccctc gagcaaattc gccggcggcg ggaccatgcc ttgcgcggcc gggccatcgc
     6361 cggcggcgcc gacgccgtga caggcgatgc agttcgcctg gaacgcattc ttgccgacct
     6421 cgactgcctg atccgtcagc gggaccgggt tcggcgtgtc gagcggaatg ccggccgacg
     6481 cgacgtccga tcgaccctgc gtgagcagga gcagaccggc cgcgatcgcg accgagccca
     6541 ggccatatcc ttcccgccga gtggaccggt tgtccgagcg ccaaccgatc acgaggccaa
     6601 tgacgccggc catggccgcc aggaagccga tcaagccggc gccattgatc gtccaggtcg
     6661 ggatactgat cgagcgcgcg cccggcttcc gctcggcggc cgtgtgcgtt agctcggccg
     6721 accattggaa cgcaccgact tgactgacgg tcaacgtcat cccccagtca cctgcaacgc
     6781 tcataccggc cgtatgcgcc tcgaacgtgt tgccggtcgt cc
//
LOCUS       DATAOZ010000546         4096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2322693, whole genome shotgun sequence.
ACCESSION   DATAOZ010000546 DATAOZ010000000
VERSION     DATAOZ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322693"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VHR64_13820"
     CDS             complement(<1..1305)
                     /locus_tag="VHR64_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX3303959.1"
                     /translation="MITSTLGISGIDGDLVQIRARELEALEERIDGRIITAGQPGWDK
                     AVLIWNAMWMARPALVVQPSSARDVAEVVAFARGHGLLVSIKGGGHNIAGTAIADCGL
                     MIDMSAMRGVAVDPVARLVQVGGGCLLGDVDQATQQYGLATVMGLVSEVGVAGLTLGG
                     GFGNLCRRFGWAVDNLDSVEIVTADGQICIASERENPDLFWAIRGGGGNFGIVTRFTF
                     RLHEVGPNVLGGLMAWGVDRAEDVFDAYQEVSQKAPRELMTGVIVTSAPPAPFVPVEW
                     HGKPIVGVLVCHSGSDTERDIAPLRRLTDPVADLVTERPYVEQQRLIDDMEPKGFNQY
                     WKAEYLPGITSNYLQMSLDTAMRRLSPHSYTITLQLGGAIADRDPGDGAVGNRDAAFI
                     GGVAAMWEDDGHDAEHVAWARRTWDQIKPYSTGGNYVNFQMPD"
     gene            complement(1302..2243)
                     /locus_tag="VHR64_13825"
     CDS             complement(1302..2243)
                     /locus_tag="VHR64_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3303960.1"
                     /translation="MITRSGARKRILAGTPVSERVLDIDGIPTSLLEAGRGPVVILLH
                     GGIECGGAYWSPVIPRLAERFRIVAPDVPGLGESVPFEKFGTERFADWLEELIRATNA
                     DQPVVVAHSLLGSFAAQFAVDHSDLLGRLVIYGAPGVGPYRAPLRLMYVAMRLSFRPT
                     PKNDERFARFALLDFDATRARDIDWFDAFSTYSLERAVVPHVKRTMKSLISLGMKRIP
                     DDELRRISTPVDLLWGEGDRMVPRSVGQAASHRMGWPMTTIANSGHAPHIEEPEQFVS
                     ALITLAERNPSVSARTNRTDAIRDVNSSDVTLIGARS"
     gene            complement(2240..2629)
                     /locus_tag="VHR64_13830"
     CDS             complement(2240..2629)
                     /locus_tag="VHR64_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303961.1"
                     /translation="MGRLSSKFAQTFERLTADHDVFAPDAFFDLNMPVWRFQLIGPDA
                     FSAQLRYINQGPINIDVFRTVATTDGFVTEHEEHQDVDGEDLTARRLWLCTVRDGRIT
                     EAIGYCSGEWDAELRARHALEAPMVRP"
     gene            complement(2795..>4096)
                     /locus_tag="VHR64_13835"
     CDS             complement(2795..>4096)
                     /locus_tag="VHR64_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3303962.1"
                     /translation="DCPERGLILVPLALQQFEEGDLPGAFATFTEIGSIGERWSSADL
                     MAVSHLGCGHALVRFGKVEQGVALMDEAMVCVTTGEVSPVLVGLIYCGVIEVCHEIYD
                     LRRARQWTDALSRWCESQPDLVPYRGECLVHRAEIMQIHGAWPDALAQVVEACERLSD
                     PPGQPAIGNAFYQRAEMLRLQGDFNGADEAYHRASEWGRTVQPGLALLRLAQGHSDAA
                     ESAIRREALEIQDRSRRCRFLPAYVEVMIGVGDARSARTAADQLAEIANEISVLPLRA
                     ASWQTSGAVLLVEGRPERALESLRRAETAWQKLGIPYEAARVRTLIGQCCRELGDEET
                     AQLEFDAALRTFRQLGAQPDAAAVEALTRKALPRPEGGLTAREVEVIRLIAAGKTNRM
                     IAAELFLSEKTVARHVSNIFSKLEITSRSAATAFAFEHRLV"
BASE COUNT          756 a         1357 c         1240 g          743 t
ORIGIN      
        1 atctggcatc tggaagttca cgtagttgcc gccggtcgag tacggcttga tctggtccca
       61 ggtcctccgc gcccatgcga cgtgttccgc gtcgtgtccg tcgtcctccc acatggctgc
      121 caccccgccg atgaacgctg cgtcccgatt cccgaccgcg ccgtctccgg gatcgcgatc
      181 agcaatcgcc ccgccgagct ggagcgtgat cgtgtacgag tgcggcgaaa gcctgcgcat
      241 cgcggtatcg agcgacattt gcaggtagtt gctcgtgatg cccggcaggt attccgcctt
      301 ccagtactgg ttgaagcctt tcggctccat gtcgtcgatc agccgttgct gctcaacgta
      361 cggccgctcg gtaaccaagt ccgcaaccgg atccgtgaga cggcgaagcg gcgcgatatc
      421 ccgctcagtg tccgatccgg agtgacagac cagcacgccg acaatcggct ttccatgcca
      481 ttcgacggga acaaagggtg cgggtggtgc ggaggtgacg atcacgccgg tcatcaactc
      541 tcgcggcgcc ttctgactaa cttcctggta ggcgtcgaag acgtcttcgg cccggtcgac
      601 gccccaggcc atcaggccgc cgagcacgtt tggcccaacc tcgtgcagcc ggaacgtgaa
      661 gcgcgtcacg atgccgaagt tcccgccacc accgcgaatg gcccagaaca aatccgggtt
      721 ttctcgctcg ctggcgatgc agatctgacc gtcagccgtg acgatctcga ccgagtcgag
      781 attatcgacc gcccagccga acctccggca gaggttgccg aagccgccac cgagcgtcaa
      841 gccggccaca ccgacctctg aaactagccc catgaccgtc gccagaccgt actgctgcgt
      901 cgcctggtcg acatcgccca gcaagcagcc tccaccgacc tgcacgagac gagccaccgg
      961 atcgacggca acaccgcgca tggcggacat gtcgatcatc agcccgcagt cggcgatggc
     1021 ggtaccggcg atgttatggc caccgccctt gatgcttacc agcaagccgt ggccgcgggc
     1081 aaatgccacg acttcggcca catcccgggc cgatgagggc tggacgacca gcgccggtcg
     1141 ggccatccac atggcgttcc agatcaggac tgccttatcc cagcccggtt gaccggccgt
     1201 aattattcga ccgtcgattc gctcctcgag cgcctccagc tcccgcgcgc gaatctgaac
     1261 caaatcgccg tcgattccgg agatgccaag agtgctcgta atcatgatct tgctcctatc
     1321 agtgtcacgt ccgatgagtt gacatcacgt atcgcatctg tgcgattcgt acgtgcggat
     1381 actgacggat tgcgctccgc cagcgttatc agagccgaca cgaactgctc gggctcctca
     1441 atgtgtggcg catgtcctga gttcgcgatc gtggtcatgg gccatcccat tcgatgactc
     1501 gcagcctgtc ccaccgaacg aggcaccatc cgatctccct cgccccacag cagatctacc
     1561 ggcgtcgaaa tgcgacggag ttcgtcgtcc ggtattcgct tcatgccgag actgatgagg
     1621 ctcttcatgg ttcgtttaac atgcggcacg actgcccgct cgagtgaata ggtcgagaat
     1681 gcgtcgaacc aatcgatgtc tcgagcgcgc gttgcgtcga agtcgagaag ggcgaagcga
     1741 gcgaagcgct cgtcattctt cggggttgga cgaaatgaca gccgcatggc cacgtacatg
     1801 agccggagcg gcgcccgata cgggccaacc cctggcgcgc cgtagatcac gagccggcca
     1861 agcaggtcag aatggtcgac tgcaaactgc gcggcaaagc ttcccagcag cgagtgcgct
     1921 acaacgaccg gctgatctgc gttcgtcgcc cggatgagtt cttcgagcca gtccgcaaat
     1981 cgctccgtcc cgaatttctc gaagggcacc gactcgccca atcccggaac atcgggagcg
     2041 acgatccgga aacgttcggc taaccgcggg atcactggcg accagtaggc tccgccgcac
     2101 tcgatcccgc catgcaggag gatgacgacc gggccccgcc ctgcttccag gagcgacgtc
     2161 gggatgccgt caatgtccag cacgcgctca gagacgggtg taccggccag aatccgcttg
     2221 cgcgcgccgg atcgggtgat cacggccgta ccatcggcgc ttcgagcgca tgccgagccc
     2281 gcagctcggc gtcccattca ccgctgcaat agccgatggc ttcggttatt cggccgtccc
     2341 ggaccgtgca gagccagagt cgcctggcgg tcaggtcctc gccgtcgacg tcctgatgct
     2401 cttcatgctc ggtcacgaag ccgtcagtgg tcgccacggt gcggaagaca tcgatgttta
     2461 tcggtccttg gttgatgtat cggagttgcg cggaaaacgc gtccggcccg atgagctgga
     2521 atcgccagac cggcatgttg aggtcgaaga atgcgtcggg cgcgaagacg tcgtggtcgg
     2581 cggtcagccg ttcaaatgtc tgagcgaact tgctgctcag ccgccccacc tcaatgctgc
     2641 ttacgttcgc tacttcccga agagtctcca tcgcgtacct cctattcgtc agattcaggt
     2701 ggcacgcacg actttaggaa gcggacggaa ggctcgcgtc gggcgaacta tccaattcgc
     2761 tgattcgatc agatgggtat ttcgatgcag ggaatcagac gaggcgatgc tcgaacgcga
     2821 aggccgtggc cgccgagcgt gaagtgatct cgagcttgct gaagatgttg ctgacgtgcc
     2881 gggcgacggt cttctcgctc aggaacagct cggcggcgat catgcggttc gtcttgccgg
     2941 ccgcaatcag gcggatgacc tcgacctccc gcgccgtcag gccgccctcc ggcctcggca
     3001 atgcctttcg agtgagcgcc tcaacggccg cagcatctgg ttgcgcgccg agttggcgga
     3061 aggtgcgcag ggcggcatcg aactccagtt gcgccgtctc ctcgtcgcca agctcccggc
     3121 agcactggcc gatgagtgtt cggactcgcg ccgcttcgta cgggattccc agcttctgcc
     3181 aggccgtctc agctcgtcgc agcgattcaa gcgcgcgctc cgggcggccc tcgacgagca
     3241 ataccgcccc actcgtctgc caggaggccg cgcggagtgg aagcacgctg atctcgttcg
     3301 caatctcggc caattgatcg gctgcggtcc tggcggacct ggcatcgccg acgccgatca
     3361 tcacttcgac gtaggcggga agaaaccggc accggcgaga gcgatcctgg atctcgagcg
     3421 cctcgcggcg aatggccgac tccgccgcgt cgctgtggcc ctgggcaagc cgcaacaggg
     3481 ccagtccggg ttggacagtc cgaccccatt cgctggcccg gtggtacgcc tcgtcggcgc
     3541 cgttgaagtc accctggaga cgaagcatct ccgcacgctg gtaaaaggca ttgccgatgg
     3601 caggttgtcc cggcggatcc gagagtcgct cacacgcctc gacgacctgc gccaatgcgt
     3661 ccggccaggc gccgtggatc tgcatgatct cggcccgatg gacgaggcac tcgccccggt
     3721 acggaacgag gtcgggttgc gattcgcacc agcgactcag cgcatcggtc cattgccggg
     3781 cgcgtcgcag gtcgtaaatc tcgtggcaga cctcgattac gccgcagtag atcagcccga
     3841 ccaggacagg tgacacctcg cccgtggtga cacagaccat tgcctcatcc atcagcgcca
     3901 cgccctgctc gaccttgccg aatcgaacaa gcgcatgacc acagcccaga tggctcacgg
     3961 ccatcaggtc ggcgcttgac catcgctcgc caattgagcc gatctccgta aatgtggcaa
     4021 aggcaccagg caggtcgccc tcttcgaatt gctgaagcgc gagcggcacc aggatcaggc
     4081 cacgctccgg gcagtc
//
LOCUS       DATAOZ010000547         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1529713, whole genome shotgun sequence.
ACCESSION   DATAOZ010000547 DATAOZ010000000
VERSION     DATAOZ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529713"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1716)
                     /gene="metG"
                     /locus_tag="VHR64_13840"
     CDS             complement(169..1716)
                     /gene="metG"
                     /locus_tag="VHR64_13840"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX3303963.1"
                     /translation="MSRGTPFYITTAIDYPNGEPHIGHSFEKISADVVARYHRLLGDD
                     VVFCMGLDENSQHIVTAAQNAGMDVRAFSDLMEETFLRAWNALEISVNAWTRTTEPRH
                     ARASTELFERAMANGDIYKATYAGWYCPNCNNYYTDAELVNGHCPLHPTVTPEWLEED
                     NYFFALSKYTDVLKKHLRDNPEFVTPSVWRPEMMALIESGLRDFSVSRQVKPDKPAWG
                     IPVPGDPGHVMYVWYDALTTYLTAIGFPDDMERFDRYWPADSQIIGKDITRFHILYWP
                     AMLMSAGLELPRRIVVHGFMTLEGQRISKTTGNTVSPVDLVTEFGADPIRYYIMRDMT
                     FTSDGDFSRANLIRRYNNDLGNDLGNLLNRVVSMIGRYRDGSVPAAGEPGDLERDVQA
                     IASGAAEQAGQLIDNWELDDALDAIWALIRRANQYLEERKPWTLAKSPDNTLALDTTL
                     WTAAEATRIAAILLAPYMPSTANRIMDQLGQTSIGDGDWGKSAWGTAQFGAVNPQGPL
                     FPRIEVE"
     gene            complement(1820..>2700)
                     /locus_tag="VHR64_13845"
     CDS             complement(1820..>2700)
                     /locus_tag="VHR64_13845"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX3303964.1"
                     /translation="TIEQARKTIGRPDRGSFIARPDAHDTERSATRILEEIPAPPFWG
                     VREPTDFSASDIWPHLDLKTLFRLHWGGKGVKDQAWEELLENEFYPRLRRMQEEVEQT
                     GWMQPRARYGYFPANSEGNDLIIFDPADHQRELTRFTFPRQPARERLCLADYFLPLDS
                     GRKDVASFQIVTMGAVADAYTDMLQAKNEYSESYFAHGLSVQSAEGLAEYLHQRVRCE
                     LGIGPETGKRYSWGYPACPDLEQHALVDQLLDVKAIGVEVTNGFQFVPEQTTAAIVIH
                     HPDAKYYALLRTGGED"
BASE COUNT          475 a          874 c          855 g          496 t
ORIGIN      
        1 gaccggcgtc gccgcaccgg tcagcagctc ccctgtcagc agcagaattg cggcaagtag
       61 aattcgcaat cctgcgggag ggatgcgtgt catcgcccga actccagtat tcggcggcag
      121 ggattacaaa gccatcttcc ccaaccgacc gccacctgtc aatccgcgct actcgacctc
      181 gattctcggg aagagcgggc cctgcggatt gacggctcca aactgtgcgg ttccccaggc
      241 cgacttaccc cagtcaccgt cgccgatcga cgtctggccg agctggtcca tgatccggtt
      301 cgcggtcgag ggcatatagg gcgcgagcag aatcgcagcg atccgggtcg cctcagccgc
      361 ggtccagagc gtggtatcga gcgccaacgt attgtccggc gacttcgcca gcgtccaggg
      421 cttgcgctcc tccagatact ggttcgcgcg gcgaatcagc gcccagatcg catccagcgc
      481 gtcgtccagc tcccagttgt cgatcaactg accagcctgc tcggcggcac cagacgcgat
      541 cgcctgcaca tcgcgttcga ggtcgcccgg ctcgcccgcc gccggcaccg atccgtctcg
      601 gtaccggcca atcatgctca cgacgcggtt gagcaggttg ccgaggtcgt tgccgagatc
      661 gttgttgtac cggcgaatca ggttcgcgcg cgagaagtcg ccatccgagg tgaacgtcat
      721 atcgcgcatg atgtagtagc ggatagggtc cgccccgaac tcggtgacca aatctaccgg
      781 cgagaccgtg ttgccggtcg tcttcgagat gcgctgcccc tcgagcgtca tgaagccgtg
      841 cacgacgatc cgccgcggca gttccagccc ggcggacatc agcatcgccg gccagtagag
      901 gatatggaag cgcgtgatgt ccttgccgat gatctgggag tcggccggcc agtagcgatc
      961 aaatcgctcc atgtcatccg ggaagccgat agcggtcagg tacgtcgtca gcgcgtcgta
     1021 ccagacgtac atgacgtggc ccggatcgcc gggtaccggg atgccccagg ccggcttgtc
     1081 cggcttgacc tgccgggaaa ccgagaagtc gcgcaggccg ctctcgatca gcgccatcat
     1141 ctccggtcgc cagaccgacg gagtcacgaa ctcggggttg tcccggagat gcttcttgag
     1201 cacatcggtg tacttgctga gagcgaaaaa gtagttatcc tcctccagcc attccggcgt
     1261 cacagtcgga tgcagcggac agtgcccatt caccagctcg gcgtcggtgt agtagttgtt
     1321 gcagttcggg caataccagc cggcgtaggt cgccttgtaa atgtcgccgt tggccatagc
     1381 tcgctcgaac agctcggtcg aggcccgggc atggcgcggc tcggtcgtcc gcgtccaggc
     1441 gttgacggag atctcgagcg cgttccaggc gcgcaggaac gtctcctcca tcaggtcgct
     1501 gaaggcacgc acgtccatgc ccgcgttctg ggcggcggtc acgatatgct ggctgttctc
     1561 gtccagtccc atgcagaaga cgacatcatc gccgagcagc cggtgatagc gggctacgac
     1621 atcggccgaa atcttctcga aggaatggcc gatatgtggt tcgccgttcg ggtaatcgat
     1681 ggcggtggtg atgtaaaagg gagtgcccct ggacacgaac ttcagccttc cggagatatg
     1741 agaaccgcgg gcaacccccg cggttcaagc catccatcag attagcacgc gagccacttt
     1801 cgacatcccg cggccaggct caatcctcgc cgccggttcg gagcagggcg taatacttgg
     1861 cgtccgggtg gtggataacg atcgccgccg tggtctgctc cgggacgaac tggaagccgt
     1921 tcgtgacctc aaccccgatc gccttgacgt ccagcagctg gtcgaccagc gcgtgctgct
     1981 caaggtccgg gcaggccgga tagccccatg agtagcgctt gccggtctcg ggaccgatcc
     2041 caagctcgca gcgcacgcgc tgatgcaggt actcggccag gccctccgcg ctctgcacgc
     2101 tcaggccgtg cgcgaagtag ctctcgctgt actcgttctt cgcctgcagc atgtcggtat
     2161 atgcgtcggc tacggcgccc atggtcacga tctggaacga cgcgacatcc tttcgcccgg
     2221 aatcgagcgg caggaagtag tcagcgaggc aaagccgctc cctggctggc tggcgcggaa
     2281 acgtgaagcg ggtcagctcc cgctggtggt ctgccggatc gaagatgatc aggtcgttgc
     2341 cctcggagtt ggccggaaaa tagccatagc gtgcgcgagg ctgcatccag ccggtctgct
     2401 cgacctcctc ctgcatccgg cgcaggcgcg gatagaactc gttctcgagc agctcctccc
     2461 acgcctggtc cttgaccccc ttgccgcccc agtgcaggcg gaagagcgtc ttcagatcga
     2521 ggtgcggcca gatgtcgctg gccgagaagt cggtcggctc ccgcacaccc cagaacgggg
     2581 gcgcggggat ctcctcgagg atgcgagtcg cggatcgctc ggtgtcgtgc gcgtccggcc
     2641 gggcgatgaa cgagccgcga tccggccgcc cgatcgtctt tcgagcctgc tcgatcgtct
//
LOCUS       DATAOZ010000548         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4423129, whole genome shotgun sequence.
ACCESSION   DATAOZ010000548 DATAOZ010000000
VERSION     DATAOZ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4423129"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..>1900
                     /locus_tag="VHR64_13850"
     CDS             21..>1900
                     /locus_tag="VHR64_13850"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3303965.1"
                     /translation="MLDNLEQVIAAASDLADLISNCPELVILATSREPLAVGGEQEYP
                     VQPLATDPDAEAVRLFAERARAILPGFALTAEIESLLAEICRRLDGLPLAIELAAARI
                     KTLPPHELLARLDRRLPVLTGGRRDAPHRQQTMRDTIAWSYDLLSSDEQALFRRLSIF
                     VGGFTLDAAETIAGDLNLDTVDGIASLVDKSLLRRVADETGQARYGMLETVREYALEQ
                     LERHGELDIIWLRHAEWVVELAGRLTPLTGPTSIEAITRMAAEHDNALAALTRAVERG
                     DAEIGLRLATGLVDLWTNQSYARGALRWLDELLAMATDQTPVNLLAYGQWEKGWFAAQ
                     QGDIATAEHAAQQALAHAAGLSDERIRAVAIEGLGIVASMRGDFDEADARFREAFTIA
                     EAIGSQPIMAGTLCNRGIVAGERGDLAAGMHLLDEALTIGRQLGDPWQLSNTLGNVSW
                     LARQQRDYRRAAAADRERLAIDRAIANHLMLVDSCSMAAGVAIRLGRAERAARLAGAS
                     ARLRDEVGIAGDSQLQRNWAEIEETIRAALGPESFQRDWAAGWAMTREDALADADAVF
                     AEEQAAPDPTSTQPAAGAAVEAGLTPRETEVLRLIARDWSNQQIADALFLSRRTVHKH
                     VE"
BASE COUNT          330 a          637 c          628 g          305 t
ORIGIN      
        1 gccaacgaaa ggcgctgctg gtgctggaca acctcgaaca ggtcatcgca gccgcctccg
       61 atctggccga cctgatcagc aattgccctg agctggtcat ccttgccacc agtcgtgagc
      121 cgctcgcggt cggcggggaa caggaatatc cggtccagcc gctcgcgacc gatcctgatg
      181 cggaagccgt ccggctgttt gccgagcgcg cccgcgccat cctgccgggc ttcgcactga
      241 ccgcggagat cgaatcgctg ctggccgaga tttgccgccg cctcgatggt ctgccactgg
      301 cgatagagtt ggccgccgcc cgcatcaaga ccctgccgcc gcatgagttg ctcgccaggc
      361 tcgaccgccg cctgcccgta ttgaccggcg gtcgccgtga tgcgccccat cgccagcaga
      421 ccatgcggga cacgatcgcc tggagttacg acctgctctc ttccgacgag caggcgctct
      481 tccgccggct ctcgatcttt gtcggcggct tcacgcttga cgcggcggag acgatcgcgg
      541 gtgacctgaa cctcgacacc gtcgacggca tcgcctccct ggtcgacaag agcctgctga
      601 ggcgagtggc agacgagacc gggcaggcac ggtacggcat gctcgaaacg gtgcgcgagt
      661 acgccctcga acaactcgaa cgtcacggtg agctggacat catctggctc cgccatgccg
      721 agtgggtggt cgaactggcc gggcgcctga cgccgttgac cggaccgacc tcgattgaag
      781 cgattacccg catggcagcc gagcacgaca atgccctcgc cgcgctgacc cgggccgtgg
      841 aacggggaga cgccgagatc ggtctgcgtc tggcgaccgg cctggtcgac ttgtggacga
      901 atcagagtta cgcgcgagga gcgcttcgct ggttggatga gctgctggcc atggcgacgg
      961 accagacgcc ggtcaatctt ctcgcctacg ggcagtggga gaaaggctgg ttcgccgctc
     1021 aacagggaga catcgcgacc gcggagcacg cggctcagca ggccttggcg cacgcggccg
     1081 gtttatccga tgagcgcatc cgcgctgtcg cgatcgaggg tctcggcatt gtcgcctcca
     1141 tgcgcggcga cttcgacgaa gcagacgcgc gcttccggga ggcgttcacc attgccgagg
     1201 ccatcggcag tcaaccgatc atggccggca ccctctgtaa ccggggcatc gtcgcaggtg
     1261 agcggggaga tctggcagcc gggatgcacc tccttgatga ggccctgacg atcgggcgcc
     1321 aacttggcga tccctggcag ctgtcgaaca cgctcggaaa tgtcagttgg cttgcccgcc
     1381 agcagcgcga ctatcggcgg gcagccgcgg cggatcgcga gcgcctggca atcgaccgcg
     1441 ccatcgccaa tcacctcatg ctggtagaca gctgcagcat ggcggccggc gttgccattc
     1501 gcctcggccg ggcagagcgc gccgcccggc tggccggtgc ttccgcgagg ttgcgcgacg
     1561 aggttggcat cgcaggggat agccaattac agcgaaactg ggccgagatc gaggaaacaa
     1621 ttcgtgctgc ccttggccct gagagctttc agcgtgactg ggccgccggc tgggcgatga
     1681 cccgcgaaga cgcactggcc gatgcagacg cggtcttcgc tgaggagcaa gccgcgcctg
     1741 accccacttc cacacaacca gccgccggag ccgccgtcga ggccgggtta acaccacgtg
     1801 agaccgaggt actgcggctg atcgcacggg actggtccaa ccagcagatc gccgacgcct
     1861 tgttcctcag ccgccgcacg gtccacaagc acgtcgagaa
//
LOCUS       DATAOZ010000549         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1799548, whole genome shotgun sequence.
ACCESSION   DATAOZ010000549 DATAOZ010000000
VERSION     DATAOZ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799548"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..854)
                     /locus_tag="VHR64_13855"
     CDS             complement(135..854)
                     /locus_tag="VHR64_13855"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3303966.1"
                     /translation="MTVSPARILPRIAHMGLRIRYAAGMDEDRTRASYDHVARTYAAQ
                     GMDDLAAKPFDRDILIEIARRVGPGGTVLDLGCGPGRVTRFLHDQDLDVTGIDLSPEM
                     IAAARTRFPELRFEVASMFALPYPDGSINGLVAMYSIIHILRADQPALFCELRRVLAP
                     GGWLLLAYHLGAQDRHVGEWVGEPVDMEFLVFAPGEIETHLVDAGFTNLEPNERDAYP
                     EHEFPLPRNYLLAQRPLTTDP"
     gene            853..1110
                     /locus_tag="VHR64_13860"
     CDS             853..1110
                     /locus_tag="VHR64_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267462181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HEX3303967.1"
                     /translation="MIELQSTITCPECGFAREETMPEDACVFFYICTNCGIRLRPKPG
                     DCCVFCSYGSVPCPPCQGAGDSGPGTEAGSQWPEVGRMGAG"
     gene            complement(1074..1766)
                     /locus_tag="VHR64_13865"
     CDS             complement(1074..1766)
                     /locus_tag="VHR64_13865"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303968.1"
                     /translation="MRTQRLSARPGPVSEPGPTGLIRLGLVEGRDGQLFVPAAYAPER
                     PVPLILTFHGAGGNAQRTINYLIPVADAAGIIVLAPDSRGQTWDIMLGDYGPDVAFID
                     RALKQTFARYAIQPERVVISGFSDGASYALSLGLMNGDLFPQIIAFSPGFMALAGPVG
                     EPRIFISHGTEDAVLPIERCSRRLVAQLDDVGYDVTYHEFDGPHTVPPAIVAEAITWL
                     LQPAPIRPTSGH"
     gene            1894..2448
                     /locus_tag="VHR64_13870"
     CDS             1894..2448
                     /locus_tag="VHR64_13870"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX3303969.1"
                     /translation="MVQISSANDAVDEIRKVRQARLFHDKPIPEDVLNELLEVARWSG
                     SSRNTQPWHFVVVTDKQMLHDLSQLRPPINWVADAPLAIAVVLDGEGAPESESYDEGR
                     LTERVLIAARLLGLGGGTAWYGDPDKEARARELLGIPAGKTARSMIVLGYPTTTTDHR
                     PNKNTPGRKPMSEIVSFGTYGVEG"
BASE COUNT          484 a          717 c          981 g          433 t
ORIGIN      
        1 tcgtcgaaat cggtgagtac gcggaagagg atctcctgct cgcgcacggt cttggtccag
       61 gcgttgtgcc aggttggggt gaatccgagc gtttcgggtg tcatggcggg attgtagggg
      121 acagggggca gggatcaggg gtcagtggtc agtggtcgct gggcgaggag gtagttgcgg
      181 ggcaggggga attcgtgctc ggggtaggcg tcgcgctcgt ttggctccag gttggtgaag
      241 ccggcgtcga ccaggtgggt ttcgatctcg cctggggcga agaccaggaa ctccatgtcg
      301 accggctccc cgacccactc cccgacgtgg cggtcttgcg cgccgaggtg atacgccagc
      361 aggagccagc cacccggcgc gagcacgcgg cggagctcgc aaaagagggc cggctgatcg
      421 gcgcggagga tgtgaataat cgagtacatc gccacgaggc cgttgatcga gccatccgga
      481 tagggcagcg cgaacattga ggcgacctcg aatcgcagct ccgggaaccg ggtccgggcg
      541 gcggcgatca tctcgggcga gaggtcgatg ccggtcacat ccaggtcctg atcgtgcagg
      601 aagcgtgtga cgcggccggg accgcagccg agatcgagca cggtgccgcc cggaccgacc
      661 cggcgggcga tctcaatcag gatgtcgcgg tcgaagggct tggccgccag gtcgtccatg
      721 ccctgcgccg cgtaggtgcg ggcgacgtgg tcgtagctgg cgcgagtccg gtcctcatcc
      781 atgccggcag cgtaccggat gcgcagcccc atgtgcgcga tacgtggcag aatccgggca
      841 ggagaaacgg tcatgattga gctgcaatcg accattactt gcccggagtg cggctttgcg
      901 cgggaggaga cgatgccgga agatgcctgc gtcttcttct atatctgcac gaattgcggg
      961 attcgtctgc gcccgaagcc cggggattgc tgtgtctttt gctcgtacgg ctcggtgccg
     1021 tgcccgccgt gtcagggggc aggggacagt ggaccgggga cagaagccgg tagtcagtgg
     1081 ccggaagtgg gacgaatggg tgcgggctga aggagccagg tgatggcttc ggcgacgatt
     1141 gcgggaggga cggtgtgagg gccgtcgaac tcgtgatagg tgacgtcgta gccgacgtcg
     1201 tcgagctggg cgacgagccg gcggctgcac cgctcgatcg gcagtacggc gtcctccgtg
     1261 ccgtgcgaga tgaagattcg cggttcgccg acaggcccgg ccagcgccat gaagccgggc
     1321 gaaaaggcga tgatctgcgg gaagagatcg ccgttcatca ggcccagcga gagggcgtag
     1381 gaggcaccgt ccgagaagcc ggagatgacg actcgctcgg gctggatcgc atagcgggcg
     1441 aaggtctgtt tcagagcgcg atcgatgaag gcgacgtccg gcccgtagtc cccgagcatg
     1501 atgtcccagg tctggccgcg cgaatcgggc gcgaggacga tgatgccggc ggcatcggcc
     1561 accgggatca ggtagttgat cgtgcgctga gcgttgccgc cggcgccgtg gaaggtgagg
     1621 atcagcggga cggggcgctc aggcgcgtaa gcggctggga cgaagagctg gccatcccgc
     1681 ccctcgacca ggccgagccg gatcaagccg gtcggtccgg gttcggagac agggccgggt
     1741 cgcgcgctga gacgctgggt tcgcatgggc gtcctccgtg cgtaggttat agccttacga
     1801 gacttcggac ttcagaggtt ggttgttggt tgatggaaat gggttccaat taccaactac
     1861 caactaccat ctaccatcta ccagggaggc gaaatggttc agatcagtag cgcgaatgat
     1921 gcggttgacg agatccggaa ggtacggcag gcgcgattgt ttcacgacaa gccgattccg
     1981 gaagatgtcc tgaacgagct gctggaggtg gcgcgctgga gcgggagctc ccggaatacg
     2041 cagccgtggc acttcgtcgt ggttaccgat aagcagatgc tccatgactt gagccagttg
     2101 cggccgccaa tcaactgggt ggccgatgcg ccgctggcga tcgcggtcgt gctggatggc
     2161 gagggtgcgc cggagagcga atcgtacgac gagggccggc tgaccgagcg ggtgctgatc
     2221 gcggcgcggc tgctgggtct cggcggcggg acggcctggt acggcgatcc cgacaaggaa
     2281 gccagagcca gggagctgct cgggatcccg gccggaaaga cggcgcgctc gatgatcgtg
     2341 ctcgggtatc cgacgacgac gacggaccac cggccaaaca agaacacgcc cggccggaag
     2401 ccgatgagcg agatcgtgag ctttgggact tacggggttg agggttaaga gttaagggtt
     2461 tagggatacg gaaaaggtag cccatgcgct cttctccgaa aaccgaaccc cgacgccggg
     2521 gtggcagtga cccggggttg aaaccccggg ctgagaacac gaagcctctc cgaggctgat
     2581 cggggtcgac gcgccaaacc ctcaaaacct aagac
//
LOCUS       DATAOZ010000550         3202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9423367, whole genome shotgun sequence.
ACCESSION   DATAOZ010000550 DATAOZ010000000
VERSION     DATAOZ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9423367"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VHR64_13875"
     CDS             <1..383
                     /locus_tag="VHR64_13875"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEX3303970.1"
                     /translation="DDSHGSKELHVVYASPGVIDAYRRDKRFPDGAVLVKEVFKTTTS
                     EMTTGTVSRADALKGWFVMVKDTANRHAESALWGDGWGWSWFDAGDRSKTTTTNCRAQ
                     CQACHVPVRASDWVYIDGYPPLKP"
     gene            446..1720
                     /locus_tag="VHR64_13880"
     CDS             446..1720
                     /locus_tag="VHR64_13880"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX3303971.1"
                     /translation="MAKEATTSEMGMSKGQTGHLYIQTNEIDNAIIHYTRQADGKLAE
                     VARIKTGGAGSGEFKPISGQDSAPNSYEGAGSVIITSDRRFLFATNGGDNSVSSFRLE
                     SDGRLTLLDVKSTGNPVEGRSGTAKSLAFAPSTRTLFVLHAFGPDHLRLMSVDAEGML
                     KTRPERYTVNTYSKRNRVSTMVVVSPNEELVLVGTTFDEPIALTGLYPDGSPILWVQR
                     AGGALHSIASNAPDPDGLAVFALQKDGSLGTARFYDAKGGSPFYIAFLNQRPNTFVIG
                     YAVGDGCSICTIEKNGSIEIGPLVKIDTSAGLPSELCWLAIAPDDRTIYATNFGYSNI
                     SSYRINGSGLEIACDPACPKVLGDGTARGLNGTVTSGPSDSWISPDGAYLYQIYGNAS
                     KLVSYSTQPDGSLKEIGSVKIPYNGSQGLAGF"
     gene            1832..2743
                     /locus_tag="VHR64_13885"
     CDS             1832..2743
                     /locus_tag="VHR64_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX3303972.1"
                     /translation="MFEAIHERSRGTSAAHPGHPGEGQSATEKFDLQRAVGEARVVFS
                     GAANGTEIIDVYQKFPMAVAFPNVDDRRRKEAVLINTSGGVAGGDEIRIEVVAQGNAS
                     VAVTTQAAEKVYRALDRPARIRTRLRAEGNARLAWLPQETIVFNQARIARQTEIDVSS
                     GTELIALEWLVLGRIESGEEVLAGHILDSWRVRVDGRLVWADGFLASDQALLQLRKTA
                     LLSNWKAIATMIYFGPGLESRLERLREIGASLECACGVTIVGAIIVVRVAAIASVDLR
                     RGLRRFLDQLDHELGSGPFGVPKMWSC"
     gene            2770..3072
                     /locus_tag="VHR64_13890"
     CDS             2770..3072
                     /locus_tag="VHR64_13890"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX3303973.1"
                     /translation="MNLTPRERDKLMIALAAIVARGRLARGVKLNYPESIALISDAIM
                     EGARDGRSVADLMSEGGRLLRRDQVMEGIPEMIREIQVEATFPDGTKLVPVHNPIR"
BASE COUNT          604 a          968 c         1044 g          586 t
ORIGIN      
        1 cagacgacag tcacggctcg aaggagcttc acgtcgtgta cgcctcgccg ggagtgatcg
       61 acgcctatcg gagggataag cgcttcccgg acggtgcagt gctcgtcaag gaggtgttca
      121 agacgacaac ctctgaaatg acgaccggta ccgtcagccg cgccgatgca ttgaaggggt
      181 ggttcgtgat ggtgaaggac accgcgaacc gtcacgcgga gagcgcgctg tggggcgacg
      241 gctggggatg gtcctggttc gatgcgggcg atcgctcaaa aacgacgacg acgaactgca
      301 gggcccaatg ccaggcgtgc catgtgcccg tgcgggcgtc cgactgggtt tacatcgatg
      361 gataccctcc gttgaagcca tagcgatggt gcaggtcacc gaaagtcatt cgatttacca
      421 aagccaatca tcaaaggagc gagagatggc taaagaggcg acgacgagcg agatgggaat
      481 gtcgaagggg cagactggcc atctctatat ccagaccaac gagatcgata acgccattat
      541 tcactacacg cggcaggcgg acggcaagct ggccgaggtc gcgcgtatca agacgggcgg
      601 cgccggctcc ggggaattca agccgatcag cggccaggac agcgcgccca actcttacga
      661 gggtgcggga agcgtcatca ttacatccga tcggcgcttt ctgtttgcga ccaatggcgg
      721 agacaattcc gtttcgagct tccgcctgga gagcgacggt cgtctcacgc tgctcgacgt
      781 gaagtcgacc ggcaacccgg tcgagggcag gagcggcacg gccaaatcgc tggctttcgc
      841 tccgtcgacg cgcaccctct tcgtgctgca tgcgttcgga ccggatcacc ttcggctgat
      901 gtcggtcgac gccgaaggca tgctcaagac gcggcccgag cgatacacgg tgaacacgta
      961 cagcaagcgg aaccgtgtct ccaccatggt cgtggtctcg cccaacgagg agcttgtgct
     1021 tgtcggcacc actttcgatg agccgatcgc gctcaccggc ttgtatcccg acggctcgcc
     1081 gattctctgg gtccagcgcg ccggcggcgc gttgcactcg atcgcgtcga atgcaccgga
     1141 tccggacggc ctcgccgtgt tcgccctgca aaaggacggc tcgctcggca cggccaggtt
     1201 ctacgatgcg aagggcggtt ctccgttcta catcgcgttc ctgaatcagc gtccgaatac
     1261 gttcgtgatc ggctatgccg tcggtgacgg atgctccatc tgcaccatcg agaagaacgg
     1321 ctcgatcgag atcggcccgc tcgtcaagat cgacaccagc gcgggcctgc cgtccgagct
     1381 gtgctggctg gccatcgcgc ccgatgatcg cacgatctac gcgacgaatt tcggctacag
     1441 caacatcagc agctaccgca tcaacggtag cggcctggag attgcgtgcg atccggcgtg
     1501 tccgaaggtc cttggcgacg gcaccgcgag gggattgaac ggaaccgtga cgagcggtcc
     1561 cagcgatagc tggatatcgc ccgacggcgc ctatctctat cagatctacg gcaacgcctc
     1621 taagctggtc agctacagca cccagccgga cggttcgctg aaggagatcg gcagcgtcaa
     1681 gatcccgtac aacggctccc agggtcttgc gggtttctga tcccggtttc gcgacacgag
     1741 gtccgggcgt ggtcggcatt gccgttccgc gcccggcctc tccctcgccg atatgcgaga
     1801 cacacacggt caatcatgag ccccgagacg gatgtttgaa gcaattcacg agcgctctcg
     1861 cgggacatcc gcggctcatc ccggccatcc gggcgaaggg cagagcgcca cggaaaaatt
     1921 cgatctgcag cgcgctgtcg gggaagctcg cgtggtgttc tccggcgccg caaacggcac
     1981 ggagatcatc gacgtctatc agaagtttcc gatggccgtt gccttcccga atgtcgacga
     2041 tcgtcggcgc aaggaggcgg tcctcatcaa tacctcgggc ggtgtcgcag gcggcgacga
     2101 gatcaggatc gaggtggtcg ctcaaggcaa tgcgtcggtc gcggtgacca cgcaggccgc
     2161 ggagaaggtc tatcgggcgc tggatcggcc tgcgcgaatc cggacgcggt taagggctga
     2221 gggaaatgcc cggcttgcgt ggcttccgca agagacgatc gtcttcaacc aggcgcgcat
     2281 cgcgcgacag accgagatcg atgtgagctc cggcaccgaa ttgattgccc tcgagtggct
     2341 ggttcttggc cgcatcgaaa gcggcgagga ggttcttgcc ggacacatcc tggacagttg
     2401 gcgcgtcagg gtcgacggcc gtctggtatg ggcagatggc ttcctcgcct ccgaccaggc
     2461 gctcctgcag cttcggaaga cggcgctgct ctcgaactgg aaggcgatcg cgaccatgat
     2521 ctatttcggt ccgggtcttg agtcccggct cgagagattg cgcgagatcg gtgcttcgct
     2581 ggaatgcgcg tgcggcgtca cgatcgtggg cgcgatcatc gtggtccggg tcgccgcgat
     2641 cgcaagcgtg gatctcagac gaggtctgcg ccgcttcctt gatcaactcg accatgagct
     2701 cggatccggt cccttcggag tgccgaagat gtggtcctgt tagcagcgcg gccaagtggg
     2761 aggagcatct tgaatctgac gccacgggaa cgagacaagc tgatgatcgc cctcgccgcg
     2821 atcgtcgcgc gcggccggct cgcgcgcggc gtcaagctga attatcccga aagcatcgca
     2881 ttgatttcgg atgccatcat ggaaggcgcc cgcgacggac gatcggtcgc ggatctgatg
     2941 tccgaaggcg gccggctgct gcgccgcgac caggtcatgg agggcatccc ggaaatgatc
     3001 cgcgagatcc aggtggaggc aacgtttccg gacgggacca agcttgtccc ggtgcacaac
     3061 ccgatccgat aggagccgat gatgcgtatc gtcccgtttg gtgccgccgc gttgagcgtg
     3121 cttgcctcgc cggccttcgc tcatcccatt ctgagcgcgg gcagttcgtt cgacgccggt
     3181 ctcgtccatc cgttcagcgg cg
//
LOCUS       DATAOZ010000551         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4423604, whole genome shotgun sequence.
ACCESSION   DATAOZ010000551 DATAOZ010000000
VERSION     DATAOZ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4423604"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VHR64_13895"
     CDS             complement(<1..187)
                     /locus_tag="VHR64_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303974.1"
                     /translation="MIIAGGVTAGFGTFFRDIITGQNDEWTFVLDTIGLLIAVLGAIL
                     DFRATGRLAEFAPGFGRR"
     gene            complement(247..1407)
                     /locus_tag="VHR64_13900"
     CDS             complement(247..1407)
                     /locus_tag="VHR64_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303975.1"
                     /translation="MSNRTPGVPPAGIPAYRQEDDRRRDEARLVTLQTQVDEVRQALR
                     ELASRQVRLEDHLKQTDGQAAQNRIQLEQFRQENQQSAQARAIDENRTRQQMADLEQH
                     LDDAVRPVRSMQAHVAELLDTNRKKTDDSGQVTRRIDEVRNLVENLQALGDRNAVVTH
                     QLRDSIDAIRSELEQVRRDIIRNEDAVKVVDQEARRRIAEVAQVTEGFGSRMDEIRSD
                     LAHSFDLIEEVKRSIVHIDPTLEELREADATTRVEVNRLQAQSVERHEMLVERIDDSR
                     ANSETHLLELRTSMDQRFERVSERIERLTEELRQVEFKASTVALSIDELKQTDAGLRR
                     EVWSLHEQRLRARLEQVQQELEQVTGNRRVMEAEARVTPDQDQPKTIRSLDM"
     gene            complement(1471..2421)
                     /locus_tag="VHR64_13905"
     CDS             complement(1471..2421)
                     /locus_tag="VHR64_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX3303976.1"
                     /translation="MTAHTTAVLTDAAFEGHDTGPHPEQPARMAAIRTELTARDLLSD
                     RPRIEFGPAPRDMVERIHDPRYLGFLDEVAAAGGAWIDPDTMCAPDSVEIAYLAAGAA
                     VRGVEAVLDGAAPRAFALVRPPGHHATADRAMGFCLINSIAVAAQAALDRGLERVAIV
                     DWDVHHGNGTEAIFEARNDVLFCSVHQFGHGFFPGTGDEFDTGVGKGAGYTVNAPLKP
                     GADNRTYEAVFRQQFGPKIEAYQPELLLVSAGFDAHVADPLGSMAVTEEGFVEMAQRV
                     LDWADRLAGGRVVAVLEGGYDKPALGRSVAAVLETFDSAS"
     gene            complement(2418..2705)
                     /locus_tag="VHR64_13910"
     CDS             complement(2418..2705)
                     /locus_tag="VHR64_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303977.1"
                     /translation="MSWERAVLIGLLVFLAGCHYVLAWYSLQDLTRRTAVRGGNRTFW
                     GLFILIVPLAGALVYGMYGPQGFLSRDRPPRATIASLEESDFEDHLRAGPR"
BASE COUNT          497 a         1002 c          917 g          520 t
ORIGIN      
        1 gccgccgtcc aaagccaggc gcgaactcgg ccaggcgtcc cgttgcccgg aagtcaagaa
       61 tggctccgag gacggcgatc aacaggccga tcgtgtccaa aacgaaggtc cactcgtcat
      121 tttgacccgt gattatgtcc cggaaaaagg tcccgaatcc ggctgtgacc ccgcccgcaa
      181 tgatcacggc gagcgtcaaa cggagcgcgg tttggggatt tcgccaccac cgccgcagca
      241 cggcgatcac atgtcgaggg agcggatcgt cttcggctgg tcctggtccg gtgtcacccg
      301 cgcctcggcc tccatcacgc ggcgattgcc ggtgacctgc tccagctcct gctgaacctg
      361 ctccagccgg gcccgcaatc gctgctcgtg caacgaccag acctcccggc gcaggcccgc
      421 atcggtctgc ttgagctcat cgatgctgag cgcgacggtg ctggccttga actcgacctg
      481 acgcagctcc tctgttagcc gctcgattcg ctcgctcacc cgctcgaagc gctgatccat
      541 cgaggtccgc aactccagca agtgcgtttc ggagttggcc cgggaatcat cgatccgctc
      601 taccagcatc tcgtgccgct caaccgactg tgcctgcaga cggttgacct cgacccgcgt
      661 cgtggcgtcg gcctcacgca gctcctcgag agtcggatcg atgtggacga tcgagcgctt
      721 gacctcctca atgagatcga aggagtgggc caaatcggag cgaatctcgt ccatccgtga
      781 gccgaagccc tcagtcacct gcgcgacctc ggcaattcgc cgtcgcgcct cctgatcgac
      841 gaccttgacc gcgtcttcgt tgcggatgat gtcccgccga acctgctcca gttcgctgcg
      901 gatagcatcg atcgagtcgc ggagctgatg ggtgaccacg gcgttgcggt cgccgagcgc
      961 ctgcaagttt tcgaccaggt tgcgcacctc gtcgatccgg cgcgtgacct gaccgctgtc
     1021 gtcggtcttt ttgcggttgg tgtcgagcaa ctcggcgacg tgcgcctgca tcgagcgaac
     1081 cggccggacg gcatcgtcga ggtgctgctc gaggtcggcc atctgctggc gggtgcggtt
     1141 ctcgtcgatc gcccgagcct gggccgactg ctggttctcc tgccggaact gctcaagctg
     1201 gatgcggttt tgggcggcct gaccatcggt ctgcttgaga tgatcctcaa ggcggacctg
     1261 ccggctggcc aattcgcgca gggcttgacg gacctcatcg acctgcgtct gcagggtgac
     1321 gaggcgggcc tcgtcgcgtc ggcggtcatc ctcctgccga tacgccggaa ttcctgcggg
     1381 gggaaccccc ggtgtgcgat tgctcatcta cgtcgtatgc gtccccatgt tgtccgggtg
     1441 gacgttgctc gtatcttcac tatacccggc tcacgacgcg ctatcgaatg tctcgaggac
     1501 ggccgcgacc gagcgaccaa gtgccggttt gtcgtatccg ccttccagca cggcaacgac
     1561 acgaccacca gccaaccgat cggcccagtc gagcacgcgc tgggccatct cgacgaagcc
     1621 ttcctcggtc acagccatgc tcccgagtgg atcggcgaca tgagcatcaa aaccggccga
     1681 gaccagcaac agctccggct ggtacgcctc gatcttcggc ccgaactgct ggcggaagac
     1741 cgcctcatag gtccggttgt ccgcacctgg cttgagtggc gcattgacgg tataccccgc
     1801 gcccttgccg acgccggtgt cgaattcatc acccgtgccg ggaaagaagc cgtggccgaa
     1861 ctggtggacc gaacagaaga gcacgtcgtt gcgcgcctcg aagatcgctt cggtcccgtt
     1921 gccgtgatgg acgtcccagt cgacgatcgc gacccgctcc agtccccggt cgagcgctgc
     1981 ctgcgcggca acggcgatcg agttgatcag gcagaagccc attgctcggt ccgcggtcgc
     2041 atggtgcccg ggcggtcgca cgagtgcgaa ggcccgcggg gccgctccat caagtacggc
     2101 ctcgacgcca cgcaccgccg cacccgccgc gaggtaggcg atctcgaccg aatccggcgc
     2161 gcacatcgta tccggatcga tccaggctcc cccggcagcc gcgacctcat cgagaaagcc
     2221 gaggtagcgc gggtcgtgga tgcgctcgac catatcccgc ggggccggtc cgaattcgat
     2281 tcgtgggcgg tcgctgagca gatcccgcgc ggtcaactca gtgcggatcg ccgccattcg
     2341 cgccggctgc tccgggtgcg gacctgtgtc gtgcccctcg aaggcggcat cggtcaggac
     2401 cgccgtggtg tgcgcggtca tcgcggcccg gcccggaggt gatcctcgaa gtccgactcc
     2461 tcgagcgacg cgatcgtggc gcgcggcggc cgatcgcgcg agaggaagcc ctgcggaccg
     2521 tacatcccgt aaacgagcgc cccggccagg ggcacgatca atatgaagag accccagaag
     2581 gtgcgattgc cgccgcggac cgccgttcgc cgggtaaggt cctgcaggct gtaccaggcc
     2641 agcacgtaat ggcaaccggc caggaagacc agcaggccga tcagcacagc gcgctcccaa
     2701 ctcatgccca atcccatgcg gcgcgaccga cgtgacgtag cgccgggccg ctttgccccg
     2761 cttccttcat ccccacgcac gatctataga gaccgaatcg agaacgaggt ttgcctccaa
     2821 tatagatgcc atcctcgagc gcgggcgggg catggtaacc ccgcgctacg tttgcagcgg
     2881 cacgtctagg gcgcggcaag gaagtcctgc acaatccagc cttcatcgtc attctc
//
LOCUS       DATAOZ010000552         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_478984, whole genome shotgun sequence.
ACCESSION   DATAOZ010000552 DATAOZ010000000
VERSION     DATAOZ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478984"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VHR64_13915"
     CDS             complement(<1..528)
                     /locus_tag="VHR64_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303978.1"
                     /translation="MTDRLTGTDGAPTKPSYLRPVTSGYSLPADNEERPTLSLGGSQA
                     VTLKTFTISAAMRGRSLVDDNDFTPDELVEILETAVKLKRMHRRGEAHSYLAGKTLGM
                     IFQHPSTRTRVSFEAGMTQLGGHALYLGMQDLQLRRGETVSDTAKVISRYCDCILARV
                     VSHDDIVELGANATVP"
     gene            complement(943..2196)
                     /locus_tag="VHR64_13920"
     CDS             complement(943..2196)
                     /locus_tag="VHR64_13920"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX3303979.1"
                     /translation="MAADSTAKKKLGKVVLAYSGGLDTSAIIPWLKENYDCEVIAFAA
                     NLGQPDDLSNLEQKALASGASMLHLRDLREEFLTEYVFPTLRAGAIYERKYLLGTSMA
                     RPLTAKHQVEVALLEGADAVAHGCTGKGNDQVRFELTYQALAPQLEIVAPWRHWHIRS
                     REDAIDYCEAHGVPVTASKTKIYSVDGNLWHLSHEGGLIEDPWNEPPVDAYDLTVAPE
                     DAPDTPEYVTVGFEEGWPVSLNGQKLSPLELMAALNEIGAKHAVGRVDLLENRLVGMK
                     SRGIYETPGGTILSNAHRELEHLVIDRMTLRQKDQLAPVYADLVYNGQWFSPLKAALD
                     AFVDKTQEVMTGEVKVKLYKGQATAVARKSPYSLFREDLATFGKDDVYRQADAEGFIR
                     LFGLPTKVAAMRDRAVKESNAEVSG"
     gene            complement(2262..>2511)
                     /locus_tag="VHR64_13925"
     CDS             complement(2262..>2511)
                     /locus_tag="VHR64_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303980.1"
                     /translation="TNQFNRHVKADAKSRHYENTTVTNSWLDKMARGSVPCASRGRHG
                     LVRFLIATMQDFTLVQDLRFKPTPPRRPRSSRTGFRSA"
BASE COUNT          403 a          793 c          813 g          502 t
ORIGIN      
        1 cgggaccgtc gcgttcgcgc caagctcgac gatgtcgtcg tggctgacga cgcgagccag
       61 gatgcaatcg caatagcggc tgatcacctt ggcggtgtcg ctgaccgtct cgccccgccg
      121 gagctgcagg tcctgcatgc cgaggtagag ggcgtgacca ccgagctgag tcatgccggc
      181 ctcgaacgag acccgggttc tcgtactggg gtgctggaag atcatgccga gggtcttgcc
      241 ggccaggtag ctgtgcgcct cgccccgccg gtgcattcgc ttcagtttga cggcagtctc
      301 gaggatctcg accagctcat ccggcgtgaa gtcgttgtcg tccaccaggc tccggccgcg
      361 catcgcggcg gagatcgtga aggtcttcag ggtcactgcc tggctccctc ccagcgagag
      421 cgttggccgc tcctcattat cggcaggcag cgagtacccg ctggtcacgg gtcgcaaata
      481 gctgggcttg gttggggcac cgtcggtccc ggtcaatcga tccgtcatct cttcgtcctt
      541 cctgctccga ctcgtcgcaa cgagggtcgg acgcacgccg ccggagcgaa tccggctgaa
      601 ggggcgagat gcgtcggtgc tcgcggtacc acccttcttg cttcccgggg aatgggaagc
      661 atctctcggc tgttgccttc gtgggagcca cccactcgac cctacttgcc gggcaaaact
      721 ccggctttct tcgggcgact gctccagggc gcgttggctg gcaatgtatc cagatcgggc
      781 ttccaccatc cccgattcgc tgatggccat cggccagcta ctggtccctg tcgtcgcagg
      841 tctgcggttg tggagtctgc gtcggcggtc gctaccgctt cgtacgaagc gtcgaccgcc
      901 gcagcggaga gcgggagaga tccccgcccg tgatgatcat gatcatccgg atacctccgc
      961 gttgctttcc ttgacggccc ggtcccgcat cgcggcgacc ttggtcggca gcccgaacag
     1021 gcggatgaag ccctcggcgt cggcctgtcg gtagacatcg tccttgccga aggtggccag
     1081 gtcctcccgg aagaggctgt agggcgactt ccgcgcgacc gcggtggcct gccccttgta
     1141 gagcttgacc ttgacctcgc cggtcatgac ttcctgggtc ttgtcgacga aggcatcgag
     1201 cgcggccttg agcggcgaga accactggcc gttgtagacg agatcggcat agaccggcgc
     1261 gagctggtcc ttctgccgca gcgtcatccg gtcgatcacc aggtgctcga gctcgcggtg
     1321 cgcgttagac agaatcgtgc cgcccggcgt ctcgtaaatg ccgcggctct tcatgccgac
     1381 cagccggttc tcgagcaaat cgacccggcc gacagcgtgc ttcgcgccaa tctcgttgag
     1441 cgcagccatg agttcgagcg gcgagagctt ctggccattt agcgaaacag gccagccctc
     1501 ttcgaatccg accgtgacgt actccggcgt gtccggcgca tcctctggag cgaccgtcag
     1561 gtcgtaggca tcgaccggcg gctcgttcca cggatcctcg atcaggccac cctcatgcga
     1621 gaggtgccag aggttgccgt caaccgagta gatcttggtc ttgctggccg tgaccgggac
     1681 gccgtgcgct tcgcaatagt cgatcgcatc ctcgcgggag cggatgtgcc agtgccgcca
     1741 cggcgcaacg atttcaagct gcggcgcgag ggcctggtag gtcagctcga agcggacctg
     1801 gtcattaccc ttgccggtgc aaccatgggc gaccgcgtcg gctccttcga gcagcgcgac
     1861 ctcgacctgg tgctttgcag tcagcggccg ggccatcgag gtgccgagca ggtatttccg
     1921 ctcgtagatc gcgccggcgc ggagggtcgg gaagacgtat tcggtcagga attcctcgcg
     1981 caagtcccgc aggtggagca tgctggctcc gcttgcgagc gccttctgct caagattgct
     2041 gaggtcgtcc ggctggccga ggttcgcagc gaaggcgatg acctcgcagt cgtagttctc
     2101 cttcagccac gggatgatgg ccgaggtatc gagcccgccg gaataggcga gaacgacctt
     2161 accgagtttt ttctttgcgg tggagtctgc tgccacggga ttgtctccgg attcaatctg
     2221 tcaacgccga ccatcgcgcg ccgtgggcga tgggatcctg cttacgccga acggaaaccc
     2281 gttcggctgc tacgcgggcg acgcggcggc gttggcttga atcgaagatc ctgcacgagc
     2341 gtgaagtcct gcatcgtggc aatcaaaaaa cggacgagcc catgtcgtcc gcgactggca
     2401 caaggtaccg aaccccgcgc cattttgtca agccacgaat tggttacagt agtgttttcg
     2461 tagtggcggg acttcgcatc cgccttgacg tgccgattga actggttcgt g
//
LOCUS       DATAOZ010000553        12225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4424180, whole genome shotgun sequence.
ACCESSION   DATAOZ010000553 DATAOZ010000000
VERSION     DATAOZ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4424180"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VHR64_13930"
     CDS             <1..1341
                     /locus_tag="VHR64_13930"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX3303981.1"
                     /translation="DRGYAIAKTLGVLGASYISWLLASLKIMAFGPDTVGFGIVLIGA
                     LSGFLIRDRLRELLRDLGNRWRLLVFIEGLFLACYLAAVTIRRANPDLWERYRGGEKP
                     MDFAYFNAVLKSTSFPPFDPWFAGGYMHYYYFGFVQWASLTRLTGIVPEVAYNLAVPS
                     VFALLCLNVWSAALALIAGIRREPGTVGWRLPTYALLAPLFVAVLGNLDLVHRIGRGE
                     FGYPSHPIGILARLGGVGDIVAGIWNATLHHPAHPPDIYWPSTRIVNGTINEFPAFSF
                     LFADLHAHLMAMPLASAALILACQLLLRRQVDQPTGRLGLRGLRPGDLVERWGRVAVE
                     AVAAGFIASALYATNTWDYPTYLALLAGAFAIRECVWARWQLTYPLVVRIGLWTVGVV
                     VAGRVLFWPFYASFYPQSGIVQQPDRTPVTAYLTIHGLFLLALAGFVVISLRST"
     gene            1405..2397
                     /locus_tag="VHR64_13935"
     CDS             1405..2397
                     /locus_tag="VHR64_13935"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX3303982.1"
                     /translation="MDESHRALSALLNGGWTALLAPAIGTTSAALIAVGLATNDLSLA
                     LVGALGLIGIAAWNCRADSIRLFVLLLAATGIGIGLVTEQVALMGDIGRMNTVFKLYL
                     QAWLLFGVAAAVAIVAVWSEPRVRHTASKWIWSAALIVLFSGAALYPAISIPARVGDR
                     FAALPAQLDGMAYMDHAIYTDAPENREPVSFPLANDRQAIDWLRANVDGSPVVLEATI
                     PGYRWGSRVSIYTGLPTVLGWDWHETQQRPGFGMMVSERRTDVQRMLGETTTFDAIRP
                     LLDKYHVQLIYIGDLERAYYDASALAKFDAAVQSGELSIIYQQSSVTIYRYQPG"
     gene            complement(2568..2765)
                     /locus_tag="VHR64_13940"
     CDS             complement(2568..2765)
                     /locus_tag="VHR64_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303983.1"
                     /translation="MATAAVTANPTTDQGPDVSELPSTGGGPTDATQSGFAWTIAALL
                     TVVAIVGGRVMVRRNRSGEGR"
     gene            2776..3114
                     /locus_tag="VHR64_13945"
     CDS             2776..3114
                     /locus_tag="VHR64_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303984.1"
                     /translation="MLVGVLVAVLVGVSVTVDVAVLVGVSVTVPVGVAVGVSVGVPVG
                     VLVGVLVGVSVGVDVGVLVGVSVGVLVGVFVGVLVGVFVGVLVGVFVGVLVGVSVTTC
                     MTAFGRFALP"
     gene            3497..4834
                     /locus_tag="VHR64_13950"
     CDS             3497..4834
                     /locus_tag="VHR64_13950"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3303985.1"
                     /translation="MQPTRQLEVRTQGILAAISALAVVAIVAFVGLRYLGDSGSGARD
                     LTPTALAATTPLPVDTTSASTPVATVVASPSPAAPTGPPPLQARDKWGQVIETITEDP
                     SAAMFRGTISRAGVYGGEAITKRPSITWNTPLNDENYSVPVVKDGRIFVGGNDDTLKC
                     FDAATGEQIWSFEARDDISSSPAVGEGRVFFGSFDGNFYAVDAETGKRLWRFETAEPI
                     HSSPAIDASIVYFGGYDRVVYAVSMLDGTLVWSAPTGGKVQSSPTVSGNLVVVGSDDG
                     YVYGLDRMTGKRLWRVKTGGAVTSTPAIVHDMVYAASSSGTVMGIRAADGEVDWEYDA
                     GSPISFSSPAVSKNTVVIGTDGGKLLGLRADNGRERWSFAAKAEIRSSPIIVGGVIYV
                     GSYDENLYAVDLKTGEALWSTDRGSIVGSPVFADGTIYVSLNSGELLALKREP"
     gene            4948..6066
                     /locus_tag="VHR64_13955"
     CDS             4948..6066
                     /locus_tag="VHR64_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEX3303986.1"
                     /translation="MPRELVAIAPRTPALREYAEPALGSNQIRIKTEFASPKHGTELV
                     GYRNDPVANRPYDYSLGAVVPRDAGEGLKAFPKPLGNMAVGVISEIGADVTQFRVGDR
                     VFGHFPIRETQTVDESQADPMPDGLTNEAAVCLDPVVMALAIRDANIKLGDSVAVFGL
                     GAIGLVTAQLARLAGAGRVIAVDPLGNRRELAQRLGADDVIDPAAGDGDVGMAIRRLT
                     GDVPESSTPRAQTRLIGGYWERPTQTSQLGVDVAIETSGSIPALHQAIRATRFGGTIC
                     MISFYGRDATGLFLGDEFHVNQLQLISARSESLPLRDTPGWTLERLVRVGLDWLTSGR
                     ISVDGIVTPVVSFEDSVDAYRAIDEHPEQSIKLGIRFP"
     gene            complement(6079..6891)
                     /locus_tag="VHR64_13960"
     CDS             complement(6079..6891)
                     /locus_tag="VHR64_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX3303987.1"
                     /translation="MKLAIQTSRLPGESLDEKFANARRYGFDGVEVSIGPTFDLAERL
                     GDIRSAMASSGLPVSAICTHSIHDPLMPDPGEREQRFAGLANLLQLADELGAEGVVSV
                     PVRRSVTFPEMANPERELPELAVNEFKRWSGMLPDGHSAVFLEPLNRYEATLLRRVEQ
                     AADLAERIGSPRVCSLADMFHMNIEESDMAKPIVAAASQLAYVHTADNNRLQPGAGCM
                     DFTPTFRALKEIEYAGYVSIECTDLGGPLAAGGPDAMLPATVAFLRERWASA"
     gene            7273..8604
                     /locus_tag="VHR64_13965"
     CDS             7273..8604
                     /locus_tag="VHR64_13965"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3303988.1"
                     /translation="MIRLPRRFLSLAMVMLMSLGMIGWYVPTTGAQDQIELRVWDQFT
                     DPAESDTADKIYSDFTAANPNIKITREAYSTDQMRQTVNTALSSGTGPDIIFYDAGPG
                     YAGVLANAGLLAPLDQYSQQYGWKDRIAPSSLEATSLGGKLYGLPLQVDLIGMYYNQG
                     LFDKEGMTVPQTLDDLVNFCKQAKDKGYTPFAFADNEGWEAFHQFSMTANQMIGPDAM
                     RNLLVNNQGSWNSPEIVTAIKSYFVDLRDAGCFSKDVNALKYDDGNSLFYSEQAMVHS
                     TGSWLANQITENMPDADIRFMPFPQLPGAKDRVWVSGVGSAYYITSGSKNQDAAAKFL
                     DFLFSPDIVSRWVSEANFIVPVQFDTSTVQVSPLFKSILDTLQQASQQGTLFGYNVDV
                     LAPPQFNDVMTNGFQAMLAGDKTPEQQAADLDAAWKEGMQGQGTPQATPGS"
     gene            8670..9599
                     /locus_tag="VHR64_13970"
     CDS             8670..9599
                     /locus_tag="VHR64_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3303989.1"
                     /translation="MTPGVAIAARPPVRQRAGRHWSRYRAGYLFILPAFLLYVVFMIY
                     PFIQSIYFTFIQWNGADPVKKWVGLDNFQALIHDDLFWTSLKHNVIWVIIGTVSPMVI
                     GMLLAMLLWTRPFGFTLFRTIYFMPQVLSSVVIALVWGWIYNPIFGILNQALDRIGLE
                     NVSRGWLGDQTWALYAVLVAAIWAETGFVFVVFLAGLQNVSKDLLEAATLDGANAWQR
                     FWNVVVPQMSNVINIVTALLLIGGFAVFDIIIVMTNGGPNHATEVIATYTYDQAFTQN
                     KVGYGAALSLVMTAITLVAAVLFIRLRERGEEL"
     gene            9603..10499
                     /locus_tag="VHR64_13975"
     CDS             9603..10499
                     /locus_tag="VHR64_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3303990.1"
                     /translation="MATTTAVGDSTLAPARAEHGRRLRLDRIGGRIIVYVVLIFFFLV
                     FFVPFIWIWSSALKTSIEIARDPFALPTTLHWENLKKAWTVGHFDQYIWNSVIYCAAI
                     VTGVVVLSCMAGYALALLPLPGRNWVALLFLLGLMVPFQSVMIPLYYLLVDFHMLGTR
                     WAFIVPGTALRLPFGIFLMRAFFRGLPPELADAARVDGAGEWSVFRQVMLPLAAPGLA
                     TLVVFQFMYTWNQFAMPFVFVQKDELRPVSLGTLFFFGRFTADRGMIAAGVTISMLPV
                     VLLYLILQRRFIEGITAGALKS"
     gene            complement(10524..11612)
                     /locus_tag="VHR64_13980"
     CDS             complement(10524..11612)
                     /locus_tag="VHR64_13980"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX3303991.1"
                     /translation="MRKVLIIAILISMFGLSGHAPARADEQQDIKDATARAEEIFQLA
                     ADQKYNAMYDLIHPDAHAVVPRTVAVNTFKELYALAEAGQSKVTGVEMGPWTWGVTGK
                     TYDYAAAVSFEQPYKENGQDKVLKDTMYLVKADDGEWRWFFGGTKEFVDLAIETFGDP
                     TEEAKTPLAQGDVIDNTINDLDAFYRDAFSYTDLKYESPQVVVVEQGDSVMTACGPAE
                     TGFWAFYCPGDATLYLDDAFLTQLGQQAPFAESFVIAHEWAHHVQTTVGLVRVENPPT
                     EWNEVYSIELELMADCMSGAWAQDVAARGLLQKGDIEQTVQFALQYLGDPPGIDPYNP
                     QAHGSAEQRRDAILGGYNEGFLACNIVV"
     gene            11732..>12225
                     /locus_tag="VHR64_13985"
     CDS             11732..>12225
                     /locus_tag="VHR64_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3303992.1"
                     /translation="MEKALTGLFGSGPEADAKTARAQDFVKRYSEGAPDQGYSTDEAV
                     GQLDQLLAHANSQQVESATKKALNNMPENQRAEFGQFVNQLHARQSGGQTTSAAPASV
                     DDISRMFGQAGGSANSVNDLFGSLLGGGLGGMVSDVVGSLFGGDDKSKSTSGSSGGGM
                     GDLGA"
BASE COUNT         2072 a         3933 c         3764 g         2456 t
ORIGIN      
        1 gatcgcggct acgcaattgc caagaccctc ggtgtgctcg gcgcgtccta catctcgtgg
       61 ctgctggcca gtctcaagat catggcgttc ggaccggaca cggtcggatt cgggatcgtg
      121 ctcatcggcg cgctctccgg attcctgatc cgcgatcggc tgcgcgagtt gcttcgggac
      181 ctcggcaatc gatggcgatt gctcgtcttc atcgaaggcc tgttcctcgc ctgctacctg
      241 gccgccgtca ccatccgccg ggccaacccg gacctttggg agcgatatcg cggcggcgag
      301 aagccgatgg acttcgccta cttcaacgcg gtgctcaaga gcacgtcctt cccgccgttc
      361 gacccgtggt ttgccggcgg ctacatgcac tactactact tcggcttcgt ccagtgggcg
      421 tccctgactc ggctgaccgg gatcgtgcct gaggtcgcct acaacctggc cgtcccgagc
      481 gtcttcgccc tgctctgcct caatgtctgg tcggccgccc tcgcgctcat cgcaggcatc
      541 cgccgcgagc cagggacagt cggttggcgg ctcccgacct acgcccttct cgcaccactc
      601 ttcgtcgccg tgcttggaaa cctcgatctc gtccatcgca tcggacgggg cgaattcgga
      661 tacccttccc atccgatcgg catcctcgcc cggctgggcg gagtcggcga catcgtggcg
      721 ggtatctgga atgcgaccct gcatcatccc gcgcatccgc ctgacatcta ctggccctcg
      781 actcgcatcg ttaacgggac gatcaacgag tttccggcct tctcgttcct gttcgcggac
      841 ctgcacgcgc acctgatggc gatgccactc gcttcggctg ccctgatcct ggcctgccaa
      901 ttgctcctga ggcgccaggt cgatcagcca actggacgac tggggttgcg tggattgcgt
      961 ccgggagatc tggtcgaacg atggggtcga gttgcggtcg aggctgtcgc ggcgggattc
     1021 attgccagcg cgctctatgc cacgaatacc tgggactatc cgacctacct ggcgctgctg
     1081 gccggtgcgt tcgcgatccg ggagtgcgtg tgggcgcggt ggcagctcac ctacccattg
     1141 gtggtgcgca tcggcctctg gacagtaggc gtcgtggttg ctgggcgagt cttgttctgg
     1201 cccttctatg cctcgttcta tccccagtcg gggatcgttc agcagcctga tcggacaccg
     1261 gtcacggcct acctgacgat ccacggcctg ttcctgctcg cgctcgccgg attcgtcgtc
     1321 atcagtctgc gctcgacgta atcacgccat tctgtccgcc gggccgtatc cgccctgacg
     1381 accgcgcgcg cttcgaccgg gttcgtggac gagtcgcacc gcgctctgtc ggcgctcctt
     1441 aatggcggct ggacagctct cctggccccg gcgatcggca cgacctcggc agcgctcatt
     1501 gcggttggac ttgccacgaa cgacctcagt ctcgcccttg ttggcgccct tggactgatc
     1561 gggatcgccg cctggaactg ccgcgcggat tcgatccggt tgttcgtgtt gttgcttgcc
     1621 gcaacaggga tcgggatcgg tctcgtcaca gagcaggtcg ccttgatggg cgatatcggc
     1681 cgcatgaata cggtcttcaa gctctacttg caagcgtggc tgctcttcgg cgtggcggcc
     1741 gcggtggcga tcgtcgccgt ctggtccgaa cctcgcgtgc gccacaccgc atcgaaatgg
     1801 atctggtcgg cagccctgat cgtcctgttc tcgggcgcgg cgctctaccc cgccatctca
     1861 attccggcac gggtcgggga ccggtttgcc gccctgcccg cccagctcga cggcatggcc
     1921 tacatggacc acgcgatcta caccgacgcg cccgagaacc gggagccggt cagcttcccg
     1981 ttagcgaacg accggcaggc gatcgactgg ctgcgcgcga atgtcgatgg ctcgcccgtc
     2041 gtgctcgagg cgacgatacc ggggtatcgt tggggatcgc gggtcagcat ctacaccggt
     2101 ctgccgaccg tgctcgggtg ggactggcat gaaactcagc agcgtcccgg cttcggcatg
     2161 atggtctccg agcggcgcac cgatgtgcag cggatgctcg gcgagacgac cacattcgac
     2221 gcaattcggc cgttgctcga caagtaccac gtgcagttga tctacatcgg cgatctcgag
     2281 cgggcgtact acgatgcctc agcgctggcc aaattcgacg ccgctgtcca aagcggcgag
     2341 ctgagcatca tctaccagca atccagcgtg accatctacc ggtatcagcc cggttagggt
     2401 ggcaatcgga agaattgtct ttgacgtagc gtggggccgc cgcgctacgt tcggagacgc
     2461 tcaacatgca tgcacgcggc tgtaatacat ccctggaaac atgattcgtg gcgttgcatt
     2521 cgggcagcca ccacattgcg ttcgggtgct gatccgggcg tgcgctgcta ccgcccctcg
     2581 ccgctccggt tccgcctgac catgacccga ccaccgacaa tggcgacgac ggtcagcagg
     2641 gcggcaattg tccaggcaaa gccgctttgg gtagcatcgg tcgggccgcc gcccgtcgac
     2701 ggcagctccg agacgtccgg tccctgatca gtcgtcgggt ttgccgtcac cgcggcggtt
     2761 gccactgcag tccccgtgct cgtcggggtg ctggtggccg tgctcgtcgg cgtatcggtc
     2821 acagtcgacg ttgcggtact cgtcggcgta tccgtgacgg tgccggttgg tgtcgcggtc
     2881 ggcgtatccg tcggcgtgcc ggtcggtgtg cttgtcggtg tattggtggg cgtgtctgtc
     2941 ggcgtcgacg ttggggtgtt ggtcggcgtg tcagttggtg tgctggtcgg cgtgtttgtg
     3001 ggtgtattgg ttggcgtatt cgtcggagtg ttggtaggag tattcgttgg ggtattggtc
     3061 ggcgtatcgg tcacgacctg catgaccgca ttcggccggt tcgcattgcc ctgaccaaca
     3121 ctcgccagat tgacggatgt gcctacttgt cccgtggggt tcatcgccga tgtcaagacc
     3181 ggcaacgcca gcgcaatacc agcgaacaga acggccgcac accgcaacat gagccttgcc
     3241 ggattcgcca ccccaacccc cgtggtccaa ctccgtagca cagacgacgc aatacgcgcg
     3301 accttaccac ttgtgactat tcgcgtcaat gcccgttctc gtccctccct gccggcgcgc
     3361 tggtccgttc cgccgggtag ccgtgccggt ttgccgattc ggtgaccact cgatttccgc
     3421 tcgccgacgc ccgcttttgc ggtaccatcg tggtcacacc accgaccgat cagcagggac
     3481 gaaggacacg cgttcggtgc aacccacgcg acaactcgaa gtgcgcacgc aaggcatcct
     3541 ggccgcgatc tcagcgctgg ccgtggtggc gatcgtcgcc ttcgtcggcc ttcgctacct
     3601 cggcgattcc ggctctggag cgcgcgacct gaccccgaca gccctggcgg ccaccacgcc
     3661 gcttccggtg gatacgacat ccgcttcgac accggtcgca accgtcgttg ccagcccgag
     3721 cccggcggca ccgaccggtc cgccgccgct ccaggcgcgg gacaagtggg gtcaggtcat
     3781 agagacgatt accgaggatc cctcggctgc catgttccgc ggcacgatct cccgcgccgg
     3841 tgtctatggc ggtgaggcga tcacgaagcg accctcgatc acctggaaca caccgctgaa
     3901 cgacgagaac tactcggtgc cggtggtcaa ggacggacgg atctttgtcg gcggaaacga
     3961 cgacacgctc aagtgtttcg acgccgcgac cggcgagcaa atctggagtt tcgaggcccg
     4021 ggacgacatc tcttcgtcgc ccgcagtcgg cgagggccgg gtcttcttcg gcagcttcga
     4081 cggcaacttc tacgcggtcg atgccgagac cgggaagcga ctctggcggt tcgagaccgc
     4141 cgagccgatt cactcgtcgc ccgcaatcga cgcctcgatc gtctatttcg gcggatacga
     4201 ccgggtcgtc tacgcggtct cgatgctcga cggcacattg gtctggtcgg ccccgacggg
     4261 tggcaaggtg caatcctcac caacagtctc gggcaatctc gtggttgtcg ggagcgacga
     4321 cggctatgtc tacgggctcg accggatgac cggcaagcgc ctgtggcggg tcaagaccgg
     4381 cggtgccgtc acctcgaccc ctgccatcgt ccatgacatg gtctacgccg ccagctcgag
     4441 cggaaccgtg atgggcatcc gggcggctga cggtgaggtc gactgggaat acgacgccgg
     4501 cagcccgatc tccttctcgt cgccggccgt cagcaagaac acggtcgtca tcgggaccga
     4561 tggcggcaag ctgcttggcc tccgcgccga taacgggcgc gagcgctgga gcttcgccgc
     4621 caaggccgag atccgctcct caccgatcat cgtcggcgga gtgatctacg tcggcagcta
     4681 cgacgagaac ctgtatgcgg tcgatctcaa gacgggcgaa gcactctggt cgaccgatcg
     4741 cggctcgatc gtcggctccc cggtcttcgc cgacggcaca atctacgtct ccctcaactc
     4801 cggcgagctg ctggccctaa agcgcgagcc ataatcaaag cccggccgga ttggtccaac
     4861 ccttcggact gtggtcctcg cgcacctccg gtatgatcaa gtccatacga ttccggcacc
     4921 tactcgcaaa tagtggagga gcatgccgtg ccgcgtgaac tagtcgccat cgctccacga
     4981 acacccgcgc tccgggaata tgcggagccc gccctcggct cgaaccaaat tcggatcaaa
     5041 accgagttcg cctccccgaa gcacggtacc gaattggtcg gctaccgaaa tgacccggtg
     5101 gcgaatcgcc cgtacgacta ttcgctcggt gctgtcgtgc cccgggatgc cggcgaaggt
     5161 ctgaaggcgt ttccgaagcc gctgggcaac atggcggtcg gtgtcatcag cgagatcggc
     5221 gccgacgtga cacagtttcg ggttggcgac cgggtattcg ggcatttccc gatccgtgag
     5281 actcaaacag ttgacgagtc acaggccgat ccgatgccgg acggccttac gaatgaagca
     5341 gccgtctgcc tcgatccggt cgtaatggcg ctcgcgattc gcgacgccaa tatcaagctc
     5401 ggtgactccg tggccgtctt cggattgggc gcgatcggat tagttaccgc tcaactcgcg
     5461 cgcctcgccg gagccggccg ggtgatcgcg gtcgatccct taggcaaccg ccgcgagctg
     5521 gcccaaaggc tcggtgctga cgacgtgatc gaccccgccg ccggcgatgg cgatgtcgga
     5581 atggcgatcc gccggctcac gggcgacgtg ccggaatcgt cgactccccg ggcgcaaacc
     5641 cgactcattg gcgggtactg ggagcgtccg acgcagacga gtcagcttgg cgtcgatgtc
     5701 gcgatcgaaa cgtctggaag tattccggcc ctccaccagg ccatccgcgc gacgcgcttc
     5761 ggcggcacga tctgcatgat ctcgttctat gggcgagatg caaccggact ctttcttggc
     5821 gatgagttcc atgtcaacca actgcagctc atctccgcgc gctcggaaag cctgccactg
     5881 cgcgatacgc ccggctggac actggaacgg ctggtccggg tgggcctgga ttggctgaca
     5941 agcggccgaa tcagcgtcga cggcattgtg acaccggtgg tttcgttcga agacagcgtc
     6001 gatgcctacc gggcgatcga cgagcatccc gagcagtcga tcaagctcgg catccgtttc
     6061 ccatgaggca gctactcgtc aggccgaggc ccatcgttct cgcaggaatg cgactgtagc
     6121 gggaagcatt gcgtcgggtc cgcccgcagc gagcggacct cctaggtccg tgcactcgat
     6181 gctgacgtaa ccggcgtact cgatctcttt cagcgcccga aatgtcggcg tgaaatccat
     6241 gcagccggcg cccggctgaa gccggttgtt gtcggccgtg tggacatagg caagctggct
     6301 cgccgcggcc acgatcggtt ttgccatgtc cgactcttcg atgttcatat ggaacatgtc
     6361 cgcgagcgag cagacccggg gtgagccgat tcgctcggcg agatccgccg cctgctcgac
     6421 cctgcgtagc aaggtcgcct cataccggtt cagtggctcg agaaagacag cggagtgacc
     6481 gtccggcagc atcccagacc agcgcttgaa ctcgtttacc gccagctcgg gcaactcgcg
     6541 ctctggattg gccatctcgg ggaatgtgac cgaccggcga accggcaccg aaacgacccc
     6601 ctccgcaccc aactcatcgg caagttgcag gaggttcgcc agtcctgcga atcgttgctc
     6661 gcgctcgccg ggatcgggca tcagcggatc atggatcgag tgcgtgcaga tcgcgcttac
     6721 gggcagtccg gatgaggcca tcgcactcct gatgtcgccc agtcgttccg cgaggtcgaa
     6781 cgtcggccca atgctgactt cgactccatc gaacccataa cggcgagcgt tggcaaactt
     6841 ttcgtccagg ctttccccag gaaggcgcga agtctgtatt gcgagtttca tgccgctggc
     6901 tcctcgttgc tgtcagcgtt cgggccgaca ccaatgcagg tcgtccgggc tggtcatcgt
     6961 agcagactcg cgtccgtgaa catgaaccac cggtgcggtc tcgcgcgagt gtttgttggc
     7021 gagtatcgat ggatatccta ctgtgataga gtccaggcag aatcacgaga accgttgcgc
     7081 gtcgttcggg gagctgacac ccgtggcggc agtccgtcaa ctggttggat caatccctcc
     7141 ccatgacgtc aaatcactgc gatgccgagc ttcggaagaa tcctgccgga tcgcgaacgt
     7201 caggaaagga ggagttggcc actgctgacg acccgccttc ggattcgccg tcacgcaaag
     7261 gagcgttttc cgatgattcg acttcctcga agatttctct cgctcgctat ggtcatgctg
     7321 atgtcgctcg gcatgattgg ctggtatgta ccaactactg gtgcccagga ccagattgaa
     7381 ctccgcgtct gggaccagtt cactgacccg gccgagtcgg acacagcgga caagatctac
     7441 tccgacttca ccgcagcaaa tccgaacatc aagatcaccc gcgaggcgta cagcacggat
     7501 caaatgcgcc aaacggtcaa taccgcgctg tcgtccggaa ccggtcccga catcatcttt
     7561 tacgatgccg gacccggata tgcgggggtc ctggccaacg ccggcttgct tgccccgctc
     7621 gaccagtact cgcagcagta cgggtggaag gaccgcattg cgccgtcctc actcgaggcg
     7681 acatcactcg gcggcaaact gtatgggctg ccgctccagg tcgacttgat cggcatgtac
     7741 tacaaccagg ggctcttcga taaggaaggg atgacggttc cgcagacgct cgacgacctg
     7801 gtcaacttct gcaagcaggc caaggacaag gggtacaccc ccttcgcctt cgcggacaac
     7861 gaaggctggg aggcattcca tcagttctcg atgacggcca accaaatgat cggcccagat
     7921 gcgatgcgga atctcctggt gaacaaccag ggtagctgga actcgcccga gatcgtcacc
     7981 gccatcaaga gctacttcgt cgacctgcgc gacgccggct gtttcagcaa ggacgtcaat
     8041 gcgctcaagt acgacgacgg gaactccctc ttctactctg agcaggcgat ggtccactcg
     8101 accggtagct ggctcgccaa tcagattacc gagaacatgc cggacgctga tatccggttc
     8161 atgccgttcc cgcagctgcc gggcgccaag gatcgtgtct gggtttctgg cgtcggttcg
     8221 gcctactaca ttacgtctgg atccaagaat caggatgccg cggccaagtt cctcgacttc
     8281 ctcttctcgc cggatatcgt gtcgcgctgg gtttcggaag cgaacttcat cgtcccggtc
     8341 cagttcgata cttcgacggt ccaggtttcg ccgctcttca agtcgatcct cgacacgctg
     8401 cagcaggcat cgcagcaagg cacgttgttt gggtacaacg tggacgtgct ggctccgccg
     8461 cagttcaacg atgtcatgac gaatggattc caggcaatgc tggccggcga caagacgccg
     8521 gaacaacagg cggccgatct ggacgccgcg tggaaggagg gtatgcaggg gcagggcacc
     8581 ccacaagcaa cgcccggctc ctgataacgg atgaacccga cggggcggga gaatctcccg
     8641 ccccgaacca gtcttgaggg gtgatgacga tgacgccagg agttgcaatc gccgcccggc
     8701 cgccggtccg gcaacgcgcc ggcaggcatt ggtcccgcta ccgcgccggt tacctgttca
     8761 tcctgccggc gttcctgctc tacgtcgtgt tcatgatcta ccccttcatc cagtcgatct
     8821 acttcacgtt catccaatgg aacggggcgg atccggtcaa gaagtgggtc ggcctggaca
     8881 actttcaggc cctgatccac gacgaccttt tctggacttc gctgaaacac aacgtgatct
     8941 gggtgatcat cggcaccgtc tcgccgatgg tgatcggcat gctcctggcc atgctgctct
     9001 ggacccggcc cttcggattc accctcttcc ggacgatcta tttcatgccc caggtgctct
     9061 catccgtcgt aattgcactc gtctgggggt ggatctacaa cccgattttc ggaatcctga
     9121 accaggccct cgaccggatc ggtctggaga acgtctcgcg cgggtggctg ggcgaccaga
     9181 cctgggcgct ctacgccgtt ctggtcgcgg cgatctgggc ggagacaggg ttcgtcttcg
     9241 tcgtcttcct ggccgggctg cagaacgtca gcaaggacct cctggaagcg gccacgctcg
     9301 acggcgccaa tgcctggcag cgattctgga acgttgtcgt gccgcagatg tccaacgtga
     9361 tcaatatcgt gacggcgctc ctgctgatcg gcggtttcgc ggtgttcgac atcatcatcg
     9421 tcatgacgaa cggcggcccc aatcatgcga cggaagtgat cgcgacctac acctacgacc
     9481 aagccttcac ccagaacaag gtgggctatg gtgcggcgct ttcgctcgtg atgacggcga
     9541 tcacgctcgt cgcggctgtc ctcttcatcc gtctccggga acgtggggag gagctctgag
     9601 ccatggccac tacgaccgcc gttggcgact caaccctcgc tccggcccgc gccgagcatg
     9661 ggcggcgatt gcggctcgat cggatcggcg ggcgaatcat tgtttacgtc gttctaatct
     9721 tcttcttcct ggtcttcttc gtgccgttca tctggatctg gtcatccgcc ctcaagactt
     9781 cgatcgagat cgcgcgcgac ccgtttgcgc ttccgaccac tctccactgg gaaaacctca
     9841 agaaagcatg gacggtcggc cacttcgatc agtacatctg gaacagcgtc atctattgcg
     9901 cggcgatcgt caccggcgtg gtcgtgctct cctgcatggc gggctatgcg ttggccctcc
     9961 tgcccctgcc gggtcggaac tgggtcgccc tgcttttcct gcttggtctc atggtgccat
    10021 tccagtcggt catgatcccg ctctactacc tgctggtgga cttccatatg ctcggcactc
    10081 gctgggcgtt catcgtgccg ggtactgcgc tccggctgcc cttcgggatc ttcctgatgc
    10141 gggccttctt ccgcgggctt ccacccgagc tggcggatgc ggcgcgggtt gacggggcgg
    10201 gcgagtggtc cgtcttccgg caagtaatgc tgccacttgc cgcgccggga ctggcgacat
    10261 tggtcgtctt ccagttcatg tacacctgga atcagttcgc catgccgttc gtcttcgtcc
    10321 agaaggatga gctgcgtccc gtctcgctcg gcacgctctt cttcttcggc cgcttcaccg
    10381 ccgaccgcgg catgatcgcg gccggagtaa cgatctcgat gctgccggtc gtgctgctgt
    10441 atctgatcct gcagcgtcgc ttcatcgagg gcatcacggc gggcgccctg aagagctagc
    10501 gatcctgcct ggcgggtcgc tggctatacc acgatgttgc aggcaaggaa gccttcgttg
    10561 tagccgccaa ggatggcgtc ccttcgctgc tcagcggagc cgtgtgcctg cgggttgtag
    10621 gggtcgatgc ccggcggatc gccaaggtac tgcagcgcaa actggaccgt ctgctcgatg
    10681 tcaccctttt gcagcaaacc gcgcgcggca acatcctgtg cccaggcgcc cgacatgcag
    10741 tcggccatca gctccagctc gatgctgtaa acctcgttcc attcggtcgg cggattctcg
    10801 acccgaacca gcccgacggt cgtctgcaca tggtgcgccc attcgtgcgc gatcacgaac
    10861 gactcggcga acggcgcctg ctgcccaagc tgggtcagga acgcatcatc cagatagagc
    10921 gtggcatcgc ccgggcagta aaaggcccag aatccggttt ctgcaggccc gcaggcggtc
    10981 atcaccgagt caccttgctc gaccacgacc acctgcggtg actcatactt cagatcggta
    11041 tagctgaatg cgtcgcggta gaaggcatcc aggtcgttga tcgtgttatc gatcacgtcg
    11101 ccctgggcca gcggtgtctt tgcttcctcg gtcgggtcgc cgaaagtctc gatcgccaga
    11161 tcgacgaact ctttcgtgcc gccgaagaac cagcgccatt cgccgtcatc ggccttgacc
    11221 aggtacatcg tgtcctttag gaccttatcc tggccgttct ccttgtacgg ctgctcgaac
    11281 gagacggccg cggcgtagtc gtaggtcttg ccggtcacgc cccaggtcca cggccccatc
    11341 tcgacgcccg tcaccttcga ttgacccgct tcggccaggg catagagctc cttgaaggtg
    11401 ttgaccgcca ccgtgcgcgg caccacggcg tgcgcatccg ggtggatcag gtcgtacatc
    11461 gcgttgtact tctggtcggc ggcgagctgg aagatctcct cggcccgcgc ggtcgcgtcc
    11521 ttgatgtcct gttgctcgtc ggcccgtgcc ggcgcgtgtc ccgacaggcc gaacatcgag
    11581 atcaggatgg cgatgatcag caccttgcgc atgtcgacat gatctcctcg gtggacgcga
    11641 aatgttgcat ggaatcgagc ctagcgtagc gttgcttgca gtccgcgttg agtcgctacg
    11701 gataatcggc gggacacgag aggagtcatc catggagaaa gcgttaaccg gattgttcgg
    11761 cagtggacca gaggcggatg cgaagacggc acgagcccag gacttcgtca agcggtactc
    11821 ggagggcgcg cccgaccaag gctattcgac cgacgaggcc gtgggccaac tcgatcagtt
    11881 gctcgctcat gccaactcgc agcaggttga gtcggctacg aagaaggcgc tcaacaacat
    11941 gcccgagaac caacgggccg agttcgggca attcgtcaac cagctccatg cccgccagtc
    12001 cggcggtcag accacctcgg ccgcaccggc gtcggtcgac gacatctcgc gcatgttcgg
    12061 gcaggccggg ggctccgcga actcggtcaa cgacctcttc ggctcgttgc ttggaggcgg
    12121 attgggcggg atggtatcgg acgtggtcgg cagcctgttc ggaggcgacg acaagtcgaa
    12181 gtcgacgtcc ggctcgagcg gcggcggaat gggtgatctg ggcgc
//
LOCUS       DATAOZ010000554         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2586190, whole genome shotgun sequence.
ACCESSION   DATAOZ010000554 DATAOZ010000000
VERSION     DATAOZ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2586190"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1561
                     /locus_tag="VHR64_13990"
     CDS             116..1561
                     /locus_tag="VHR64_13990"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX3303993.1"
                     /translation="MIVLKFGGTSVGSLEKVRDAAAIIEQQPEPRAAVVSAASGVTNL
                     LLEAAQYAAAGDTAALDHVLELITTKHTAILDGIADKHERAGAVGELDQLYAALHDAL
                     GTVTVAGEVSKELSDRIVSTGEKSMTVMLAALLRVGGTPAVHVFADTVIGTDDRFGNA
                     RPDRDRTRAQAQERVRPHLDAGKTVIMTGFIGGAPDGRTTTLGRGGSDYSATLLGAAL
                     DADEVQIWTDVPGVLSADPRQVPEARVVPAISYEEAQELAHFGAKVLHPRTIRPAVAR
                     DIPVRILSTFAPTEPGTLVQRKTSDASIKAVTAMKGLMMLSIDVPELEDLAGAAASVF
                     QQLHVDRVEIVHVAQASSRRRMTYLVDAASAGGCATTVQALDTALDDYEAAIACDEQV
                     ALVAAVGEGAADKPATLSKMMEVLRKAGVPVLGSSQQTSNVALVVVVPALRAQKAVEV
                     VHEAFIGKQPASASGRRPRRTRMQRESLRVG"
     gene            1587..2690
                     /gene="asd"
                     /locus_tag="VHR64_13995"
     CDS             1587..2690
                     /gene="asd"
                     /locus_tag="VHR64_13995"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX3303994.1"
                     /translation="MGTDRIPVAVLGATGSVGQRFIQLLANHPWFEVAELVASDRSTG
                     KRYAEATEWRLDSNMPAGAADLVVKDYGEELSSPVAFSALPGEVAGEIEQRLARAGHA
                     VLSNSSAHRMEPDVPLMIAEVNPESVAAIETQKANRGWAGYIVTNGNCSAIHLTLTLK
                     PLHDAFGLDAVMVTTMQAVSGAGYPGVPSLDMIDNVVPFISTEEEKMAEEARKFLGTW
                     DGEFRQGDFVVSASCNRVPVRDGHTEAVSVRFRNQASVDEVREALASFRGKPQELELP
                     SAPSRPIVVRSERNRPQPVLDRDCEKGMASVVGRLRECPILGIKYTLLGHNTIRGAAG
                     ASILNAELLKVEGLLPQYDHSRGRLVAGAAVPA"
BASE COUNT          487 a          940 c          906 g          431 t
ORIGIN      
        1 gcacgcctgt cgggtggatg gaatagcgaa gaaatcgcag cagcaatcca acgacacgac
       61 aggcgggagc gaaagccccc gcttttttgt tgcctactgc gaacaggagc ggcagatgat
      121 cgtactcaag tttggcggca cctcggtcgg gagcctggaa aaggtgcgag atgccgccgc
      181 cattattgag cagcaacccg agccgcgcgc ggctgtcgtc tcggccgcca gcggcgtgac
      241 caacctgctc ctcgaggccg cccagtacgc ggcagccggc gataccgccg cgctcgacca
      301 cgtcctcgaa ctcatcacca ccaagcacac ggccatcctc gatggaattg ccgacaagca
      361 cgagcgggcc ggcgcagttg gcgagctcga tcagctctac gccgcactgc acgacgccct
      421 gggcaccgtg accgtcgccg gcgaggtcag caaggagctc tcggaccgga tcgtctcgac
      481 cggcgagaag tcgatgacgg tcatgctggc cgcccttcta cgcgttgggg gcacgccggc
      541 cgtccatgtc tttgccgaca ccgtgatcgg cacggacgat cgcttcggca atgcccggcc
      601 ggacagagac cgcacgcgcg cccaggccca ggagcgcgtc cgcccccacc tcgatgccgg
      661 caagaccgtg atcatgaccg gcttcattgg cggcgcgccg gacggccgca cgaccacgct
      721 cggccgcggc ggctccgatt acagcgcgac cctcctcggg gccgccctcg atgccgacga
      781 ggtccagatc tggaccgatg tgccgggcgt gctcagcgct gaccctcgcc aggtcccgga
      841 ggcgcgcgtc gtgcccgcta tttcatatga agaggcccag gagttggccc atttcggagc
      901 gaaggtgctc cacccccgga ccatccgccc cgccgtcgcc cgcgatatcc cggtccggat
      961 cctctcgacc tttgcgccga ccgagccggg cacgttggtc cagcgcaaga cctcggacgc
     1021 gagcatcaag gccgtcaccg cgatgaaggg actgatgatg ctctcgatcg atgtgccgga
     1081 gctcgaggac ctggcgggtg cggcggcatc cgtgttccag cagctccacg tcgaccgggt
     1141 cgagatcgtc catgtcgccc aggcctcatc ccgccggcgc atgacctacc tggtcgatgc
     1201 tgcttcggcg ggtggctgcg ccacaacggt acaggccctc gacactgcgc tcgacgacta
     1261 tgaggcggca attgcctgcg atgagcaggt cgccctcgtc gcggccgtcg gcgagggtgc
     1321 ggccgacaag ccggccacgt tgagcaagat gatggaggtc ttgcgcaagg caggagtgcc
     1381 ggtactcggc tccagccagc agacctcgaa tgtggccctg gtcgtggtcg tgccggccct
     1441 gcgcgcgcaa aaggcggtcg aggtcgttca cgaggccttt attggaaaac aaccggccag
     1501 cgcgtcagga cgacgcccac gccggacccg gatgcagcgc gaatcgctgc gcgtcggcta
     1561 attaacatcg aagaaaggac atcacgatgg gtacggatcg aattccagta gcggtactcg
     1621 gggcaacggg cagtgtcggc caacggttca tccagttgct tgcgaaccac ccgtggttcg
     1681 aggtcgccga gctcgtggca tcggaccgtt caaccggcaa acgctacgcc gaagcaaccg
     1741 agtggcgcct cgattccaac atgccggccg gcgcggctga cctcgtcgtc aaggattacg
     1801 gcgaggagct gagcagcccg gtcgccttct cggcactgcc cggagaggtc gcgggagaga
     1861 tcgaacagcg gctggccagg gccggtcacg ccgtcctctc gaacagctcg gcccaccgga
     1921 tggagccaga cgttcccctg atgatcgccg aggttaaccc ggagagcgtc gccgcgatcg
     1981 agacgcagaa ggcgaaccgc ggctgggctg gctacatcgt caccaacggc aactgctccg
     2041 cgatccacct gaccctgacc ctcaagccgc tccacgacgc cttcgggctc gacgcggtca
     2101 tggtcacgac catgcaggcc gtctcgggtg cgggctaccc gggcgtgccg tccctcgata
     2161 tgatcgacaa cgtcgtcccg ttcatctcga ctgaagagga gaagatggcc gaggaggcgc
     2221 gcaagttcct cggtacgtgg gacggggagt tccgacaggg tgatttcgtg gtcagcgcct
     2281 cgtgcaaccg cgttcccgtg cgggacggtc acaccgaggc ggtgtcggtc cgcttccgca
     2341 accaggcgag cgtcgatgag gtccgcgagg cgttggcatc attccggggc aagccgcagg
     2401 agctggagct gccgagcgcg ccgagccggc caatcgtcgt tcgctccgag cgcaaccgtc
     2461 cgcagccggt gctcgatcgg gattgcgaga agggcatggc ttcggtcgtc ggccgcctgc
     2521 gcgagtgtcc gatcctcggc atcaagtaca ccctgctcgg ccacaacacg atccgtggcg
     2581 cggccggcgc ctcgatcctg aacgccgagc tgctcaaggt cgaaggcctc ctcccccagt
     2641 acgatcacag ccgcggccgc ctcgtcgccg gggctgcggt tcctgcttga tccgtcgttg
     2701 acatgctctc cggcgcgaga ctacgctacc ggggtgattc acgacgacag gggcgaacca
     2761 taca
//
LOCUS       DATAOZ010000555         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1800756, whole genome shotgun sequence.
ACCESSION   DATAOZ010000555 DATAOZ010000000
VERSION     DATAOZ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800756"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1202)
                     /gene="recA"
                     /locus_tag="VHR64_14000"
     CDS             complement(150..1202)
                     /gene="recA"
                     /locus_tag="VHR64_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX3303995.1"
                     /translation="MTERDRAIELAISQIERQFGKGAIMKMGEANSHMQIDAIPTGSI
                     ALDLALGIGGIPRGRITEIYGPESSGKTTLALHVIAEAQRLGGIAAYVDAEHALDPLY
                     ASRLGVNLEELLISQPDTGEQALEIVDTLVRSSAIDVIVIDSVAALVPKAEIDGDMGD
                     SHMGLQARLMSQALRKLTGNISRTKTSVIFINQLRSKIGVMFGSPETTTGGNALKFYC
                     SVRMDIRRIETIKNGTDSIGSRARVKVVKNKVAPPFRQAEFDIMYNEGISVAGSILDV
                     ATDMGIVKKSGAWFNLGDERLGQGRENAKTYLKHNPDVLNSIKQQIMGGTPELAGRLA
                     FAGAEEDGVIPDGVDD"
     gene            complement(1337..1678)
                     /locus_tag="VHR64_14005"
     CDS             complement(1337..1678)
                     /locus_tag="VHR64_14005"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3303996.1"
                     /translation="MVAERVIDERPGVFEALADPTRRWMIQRLARSGSVTPTALAEEL
                     PISRQAVSRHLSVLQHGGLVTVHKEGREQRYRLAVDPLRDAAAWIAGIEQEWDTRLAA
                     LGDLLNEKSGS"
     gene            complement(1668..2135)
                     /locus_tag="VHR64_14010"
     CDS             complement(1668..2135)
                     /locus_tag="VHR64_14010"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3303997.1"
                     /translation="MVSAVQNTIEREVVYPQPVERVWSALTEADQLKRWFGSKTAEID
                     LRPGGAMHLVWEGMTARAVVETVDPTSYFSWRWKPGSVADDLTRPLAEQEPLTRVEFM
                     LESVEAGTRLRMVESGFAAFDESQRVSALGQNTEGWTECFRGLEALLGSADGR"
BASE COUNT          402 a          756 c          698 g          440 t
ORIGIN      
        1 tcagccgact tccgattctg agccggggac ttgagtcccc ggcggagctg cgataaagaa
       61 gtggcaatcg ttgccacggc tgcttcaaat gacaaaccgg gcgagaatca tttctcgccc
      121 ggtttgttac ctgcctgttc gcggaatatt cagtcgtcaa ccccgtcggg gatgacgccg
      181 tcttcctcgg ctccggcgaa ggcgaggcgg ccggccagct ccggcgtgcc acccatgatc
      241 tgctgcttga tcgagttcag cacgtccggg ttgtgcttga ggtacgtctt cgcgttctcc
      301 cggccctgac ccagccgctc gtcaccgagg ttgaaccagg cgccactctt cttgacgatg
      361 cccatgtcgg tggcaacgtc gagaatcgag ccggcgaccg agatgccctc gttgtacatg
      421 atgtcgaact cggcctgccg gaagggcggg gcgaccttgt tcttgaccac cttgacccgc
      481 gcccgcgagc cgatcgagtc ggtcccgttc ttgatcgtct cgatccggcg gatgtccatc
      541 cggacggagc agtagaactt gagcgcgtta ccgccggtgg tcgtctcggg cgagccgaac
      601 atgacgccga tcttgctgcg gagctggttg atgaagatga cggaggtctt ggtccggctg
      661 atgttgccgg tcagcttgcg gagcgcctgg ctcatcagcc gcgcctgcaa gcccatgtgc
      721 gaatcgccca tgtcgccgtc gatctcggcc ttcgggacca gcgcggcgac ggagtcgatc
      781 acgatcacgt cgatcgcgct cgagcggacg agagtatcga cgatctcgag ggcctgctcg
      841 ccggtgtccg gctgggagat cagcagctcc tcgaggttga cgcccaggcg gctggcatag
      901 agcgggtcga gcgcgtgctc ggcgtcgaca taggccgcga tgccgcccaa gcgctgggcc
      961 tcggcgatga cgtgcagggc gagggtcgtc ttaccggacg actcggggcc gtagatctcg
     1021 gtgatccggc cgcgcgggat gccgccgatg ccgagagcaa ggtcgagcgc gatcgatccg
     1081 gtcgggatcg cgtcgatctg catgtgcgag ttggcctcgc ccatcttcat gatcgcgccc
     1141 ttgccgaact gccgctcgat ctgggaaatc gcgagttcga tagcgcggtc gcgctcagtc
     1201 attggatgtc ctttataggt gcctttagaa caagtattct ggtaaatagt agtccacgga
     1261 gggccggaaa acaaggggaa aagtagtcag taaccggtgg tcagtagccg gcacgtccgg
     1321 tccacaaacc ggaaatctag ctgcccgact tctcgttgag gaggtccccc aacgccgcca
     1381 accgggtatc ccactcctgc tcgatgccgg caatccaggc cgcggcatcc cgcagcggat
     1441 cgaccgccag tcgataccgc tgctcccgcc cctccttgtg gaccgtgacc agcccgccat
     1501 gttggagaac actcaggtgc cggctgacgg cctggcggga gattggcagc tcctccgcca
     1561 gggccgtcgg cgtgactgat ccgctccggg ccaggcgctg gatcatccag cgcctggtcg
     1621 ggtcggccag cgcctcgaag acgcccggcc gctcgtcgat gacccgctca gcgaccatcc
     1681 gcgcttccca gcagtgcttc cagacccctg aagcactcgg tccagccctc ggtgttctgg
     1741 ccgagcgcag agacgcgctg cgactcgtca aaggccgcga atccggattc caccatgcgc
     1801 aagcgcgtgc cggcctcaac cgattcgagc atgaattcga cccgtgtcag cggctcctgc
     1861 tctgccagtg gtcgagtcag atcgtcagca acagatcccg gcttccagcg ccaggagaag
     1921 taactcgtcg gatcgacggt ctcgacgact gcccgggccg tcatgccctc ccagaccaaa
     1981 tgcatcgctc cacccggacg caagtcgatc tcggcagtct tggagccgaa ccacctcttc
     2041 aactgatctg cttccgtgag cgccgaccag acgcgctcga ccggctgcgg atagaccact
     2101 tcgcgctcga tcgtgttctg taccgccgat accatcgatc ttctcccaag atacgcaacc
     2161 aatgtgttgc gtatctaacg tagcatacga ttatcgaacg cgcaactaac cggttgcgcg
     2221 ttcgtcttcc tggaactcgg tctacagttt ccggtccctc aaccctcaac cctccaccag
     2281 ccaggtccgg ccagtc
//
LOCUS       DATAOZ010000556         7030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_742489, whole genome shotgun sequence.
ACCESSION   DATAOZ010000556 DATAOZ010000000
VERSION     DATAOZ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742489"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VHR64_14015"
     CDS             <1..645
                     /locus_tag="VHR64_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX3303998.1"
                     /translation="RLVRAGLTTILGRETATNVAVIFGYVIAAAMFVVGIWLMQPTLP
                     LIALFVMYLAYVEGKGVRIESMMRRIRVGQFSLWDGGGIAPEVPLKLALAGGARDVAV
                     VDGGRVVGMLWRRDVLKAVRAGAANYLVGDVMDRQIITAESNLTVHDVHIAMERANRW
                     AIPVVEDGVYRGIFTSDRLMHVYKIVSDQTSGRKRLMQLWATIGARLRGEAGPA"
     gene            737..1195
                     /locus_tag="VHR64_14020"
     CDS             737..1195
                     /locus_tag="VHR64_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX3303999.1"
                     /translation="MKVSTRGEYGVRAMVALAKHAGDRPVSIGVISKESAVPYAYLEQ
                     LIVPLRKAGLVESKRGAHGGYTLARAADEIRVGEVYRAMEGPVAPMDCVSENLADQTC
                     PLIPGCQTRPVWVKVRDSIIDAIDSITLADLLEGPSEVEANRAPVAVKAS"
     gene            1230..2396
                     /locus_tag="VHR64_14025"
     CDS             1230..2396
                     /locus_tag="VHR64_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX3304000.1"
                     /translation="MSSIYLDYAATTPLDPDVLRAMMPYLGPEFGNPSSIYQQAQAAK
                     AAIEDARQKVAAVFALDPAEIVFTSGATESDNLALSGVAWAAKKAGIERPHIVSTAIE
                     HSAIIESLAWLESVGFAVTLVGCDRDGMIDPADVRREIMKDTALISVMYANNEVGSIQ
                     PIPEIAAIAHESGIPLHVDATQAAGLLDLSSGTLHADLVSISAHKFYGPKGVGVLAAR
                     RDVRIDWQQHGGGQEGGRRGGTENVAGIVGLATALVRADGYRDAYAAHCRSVRDLLWE
                     RISGEIDDIWLNGPSLDGPRLPNNLNIGIPGVQGETMLVNLDLAGIAASAGSACSVGR
                     NEPSHVLLAMGRTVEEARSSLRLTVGRPTSQADVIEAADAVVEVADRVRDLARR"
     gene            complement(2406..3056)
                     /gene="scpB"
                     /locus_tag="VHR64_14030"
     CDS             complement(2406..3056)
                     /gene="scpB"
                     /locus_tag="VHR64_14030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX3304001.1"
                     /translation="MSTVPTDSLIQERFRTDDAFLSDHELMAAIEALLMVADGPVHPA
                     DLAQAIDVDVRDVAVALTQFEMQTGRGWILQRHGEMVQLTTAPRFAEYVRRFLGIERQ
                     SRLSSAALESLAIIAYRQPVTRSEIEAVRGVDCTGVLATLIQRNLVEAVGRQDSPGNP
                     ILYGTTPEFLMHFGLGSLDELPPLGDVNGQDANLHLADLVAQSHADVDVHADIAET"
     gene            complement(3068..3850)
                     /locus_tag="VHR64_14035"
     CDS             complement(3068..3850)
                     /locus_tag="VHR64_14035"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX3304002.1"
                     /translation="MNSVAVESALFQGYQLRLPAFEGPFDVLLRLVERSHLAITDVSL
                     VAVTSQFLAYVETLDQSDAELIAEFSSVGSRLVVLKSRSLLPRPPVEEVEQVSDLTRE
                     LIEYRAVKEMATLLKERDARGSGAFPHARAPLPADDRTVLRPLAAHQPLQLARALRRR
                     LSVVPAPSLIVAARKLVSLRDMAARVLAGLQHSGQASFRSITRHCEDLHEARTAFLAV
                     LVLARRQVVDVDQDELFGEITIRSRSAAANGDTFLNSLSFED"
     gene            complement(3905..4444)
                     /locus_tag="VHR64_14040"
     CDS             complement(3905..4444)
                     /locus_tag="VHR64_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304003.1"
                     /translation="MIDLLHLTSPTHHPVIHRLHQIWLALTLRTAPDVDPTLLPNEHV
                     CRLFQGLHPHDQRHLIAVYEKAVAAGLPPDVCTAGLLHDIGKVTLAGTRISLAARIAH
                     VLLQHFSPSLEARMIERSGPWLGVGLRLAKNHGHLGADRLRALAVEPGICEIVERHDD
                     CTHPDPAVRLLQEIDSATP"
     gene            complement(4441..5181)
                     /locus_tag="VHR64_14045"
     CDS             complement(4441..5181)
                     /locus_tag="VHR64_14045"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX3304004.1"
                     /translation="MRTGWDPRQLFILGLLIFVLPNLLQGGWNIPPSDQIAAYLLSGI
                     IAITVHEFMHAWSAHMFGDDTALMMNRVNLNPANHFDPLGFFGFVLISLGFTSIAWGK
                     PVPVNFNRLRGDFRQRKLESLVIAGCAPLSNVVMACCATLIFRGLDGQNRDLGFTGTF
                     LIAFITLNILLAAFNLIPLPPLDGYRVLTAILPNFWYPTFAKLEQFGLFLPFVLIFID
                     GRLNLGIYNELIGPGYRLLLDLVRWLLG"
     gene            complement(5202..6224)
                     /locus_tag="VHR64_14050"
     CDS             complement(5202..6224)
                     /locus_tag="VHR64_14050"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX3304005.1"
                     /translation="MADPISTTPNGSGDALLKVDNLVMHFPLTQGIIFQRKVGAVQAV
                     DGISFEVKKGETLGLVGESGCGKSTTGRAILQLYKPTSGEIVFRGKDLTKLPGGEMRK
                     MRRHLQMIFQDPYASLNPRMTVGSIIAEPMQIHGLVDKKDRNKRVQDLLQTVGLNPYF
                     ANRYPHEFSGGQRQRIGIARALAANPDFIVADEPVSALDVSIQAQIVNLLEDLQDQFG
                     LTYLFIAHDLSVVKHISDRVAVMYLGKIVEMASRNELYDQPLHPYTKALLSAVPIPDP
                     VVEKRRERIILTGDVPSPINPPSGCHFHTRCPYVMDVCKQIDPVFANQGNGHFVACHL
                     YPGSGK"
     gene            complement(6228..>7030)
                     /locus_tag="VHR64_14055"
     CDS             complement(6228..>7030)
                     /locus_tag="VHR64_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3304006.1"
                     /translation="ILFYGDDILRMSDEQVRSIRGNDIAMIFQDPMTSLNPVLTISRQ
                     LCEAIQLHLGLNKDQARARAVDLLKMVGIPNAEKRLDQYPHQFSGGMRQRVMIAMALS
                     CNPKLILADEPTTALDVTIQAQILDLMRTLQSERDTGVILITHSMGVVAGMADRVQVM
                     YAGHIVETATTEEIFANPRHPYTVGLMKSIPRLDATTKEKLEPIRGLPPDLIDLPDMC
                     PFVPRCNYAREKCEQKNPPLLEVAPGHYSACWFWDEVNKDNEPPRRDA"
BASE COUNT         1314 a         2069 c         2353 g         1294 t
ORIGIN      
        1 cggttggtcc gcgccggact gacgacgatc ctcggccgcg agacggcaac caacgtggct
       61 gtcatcttcg ggtacgtcat tgcagcggcc atgttcgtgg tcgggatctg gctgatgcag
      121 ccgaccctgc cgctcatcgc gctcttcgtg atgtacctgg cgtatgtcga aggcaagggc
      181 gtccgcatcg agagcatgat gcgccgaatc cgggtcgggc agttttcgct ctgggatggc
      241 ggcggaatcg cgcccgaggt gccgctcaag ctcgctctgg ctggcggcgc gcgtgatgtc
      301 gcggtcgtcg atggcggccg cgtggtcgga atgctctggc ggcgcgacgt gctcaaggcg
      361 gtccgggccg gagcggccaa ttatctggtc ggtgacgtca tggatcgcca gatcatcacg
      421 gccgagagca atttgacggt acacgacgtc catatcgcca tggagcgggc gaatcggtgg
      481 gcgatcccgg tggtcgagga tggcgtctat cgcggcatct tcacgtcgga ccggttgatg
      541 cacgtctaca aaatcgtcag tgatcagacg tcgggccgaa agcggctgat gcagctctgg
      601 gcgacgatcg gcgcgcggct tcgcggcgag gcaggccccg cctgagcggc ctcacgcgct
      661 tccctcggtt gaacccaatg cgaaacaagg gtaggatgaa ttggacgtgc ataggtttgc
      721 acgctccgga ggcgccatga aagtctcaac tcgaggcgag tacggggtca gggccatggt
      781 tgccctggcc aagcatgctg gcgatcgacc cgtttcgatt ggtgtcatat ccaaagaatc
      841 cgcggtccca tacgcctacc tcgaacaact catcgtgccg cttcgcaagg ccggcctcgt
      901 cgagagcaag cggggcgccc acggtggata tacgctggcc cgtgccgcgg acgaaatccg
      961 ggtgggagag gtctaccggg cgatggaagg tcccgtcgcg ccgatggact gcgtctccga
     1021 gaatctcgcc gatcagacct gcccgttgat tcccggctgc cagactcggc cggtctgggt
     1081 caaggtgcgg gactcgatta tcgacgccat cgattcgatc acgcttgctg acctcctcga
     1141 gggaccgtcg gaagtggaag cgaaccgcgc gccggttgcc gtcaaggcaa gctagcggat
     1201 tccactcctc atagtccagg atgggccggg tgtcctccat ttatctcgac tacgccgcca
     1261 caacgccact tgatcccgat gtcttgcggg ccatgatgcc gtatctcggc ccggagttcg
     1321 gcaacccgtc aagcatctac cagcaggctc aggcggccaa ggcggccatc gaggacgcgc
     1381 gtcaaaaggt ggccgcggta tttgcgctcg atccggccga gatcgtcttc accagtggcg
     1441 cgactgagag cgacaacctg gcactatccg gcgttgcctg ggccgcgaag aaggcaggaa
     1501 tcgagcgccc gcacatcgtc tcgacggcta tcgagcactc cgcgatcatc gagtcgctcg
     1561 cctggctcga gtcggttggt tttgcggtca cgctggtcgg atgcgatcgg gacggaatga
     1621 tcgatcccgc ggatgtgcgc cgggagatca tgaaagacac ggcgctgatc agcgtcatgt
     1681 acgcgaacaa cgaggtcggc tcgatccagc cgatcccgga gatcgcggcc atcgcgcatg
     1741 agtccgggat cccgctccac gtcgatgcga ctcaggcggc cggcttgctt gacctgagtt
     1801 cgggtacgct ccatgccgat ttggtctcga tctccgcgca caagttctat ggaccgaaag
     1861 gcgtcggtgt gcttgccgcg cgacgggatg tccgcatcga ctggcagcaa cacggtggcg
     1921 ggcaagaggg cggccgtcgc ggtgggaccg agaacgtcgc cggaatcgtc ggattggcga
     1981 cagcgctggt tcgggccgat ggctaccgag atgcctatgc agcgcattgc cggtcggtgc
     2041 gggatttgct ctgggagcgg atctcgggcg agatcgatga tatctggctg aacggtccct
     2101 cgctcgatgg tccgcgtttg cccaacaatc tcaacatcgg tatcccgggc gtccagggcg
     2161 agacaatgct cgtcaacctg gatcttgccg gcatcgcagc ttcggcggga tctgcctgct
     2221 ccgtcgggcg gaatgagccg agtcacgtat tgctggctat gggcaggacg gttgaagagg
     2281 cgcgctccag cctgcgctta accgtcggga ggccgacctc ccaggcggat gtgatcgaag
     2341 cggctgacgc agtcgtcgag gtcgccgatc gggttcgcga cctcgcgcgc cgatagggtt
     2401 gcgactcaag tttcggcgat atccgcatgc acatcgacgt cagcatgcga ctgggcgaca
     2461 agatcggcca ggtgcaggtt tgcgtcctgc ccgttgacgt cgcccagagg cggcaactcg
     2521 tcgagcgagc cgagcccgaa gtgcatcagg aactcgggcg tcgtgccgta gaggatcggg
     2581 tttccgggcg agtcctgccg tccgactgcc tccaccaaat tgcgctgaat gagtgttgcg
     2641 agcacgccag tgcaatcgac gccgcgaact gcttcgatct cggagcgggt aaccggctgg
     2701 cggtaggcga tgatcgccag cgactccagc gcggctgacg agagccttga ttgccgctcg
     2761 atgccgagga agcggcgaac gtactcggcg aaccgaggcg ccgtggtcag ttgaaccatt
     2821 tcaccgtgac gctggaggat ccagccgcgc ccggtctgca tctcgaattg cgtcagggcc
     2881 accgcaacat ctcgcacgtc aacatcaatc gcttgtgcga ggtcagcggg atgaacgggt
     2941 ccatcagcca ccatcagcag cgcctcgatc gcggccatga gttcgtggtc gctcaagaag
     3001 gcgtcgtcgg ttcggaatcg ttcctgaatc aggctgtcgg tcggcacggt actcatgctt
     3061 ttaccaccta atcttcaaag gaaaggctgt tcaggaacgt gtcgccgttt gctgcggccg
     3121 accggctgcg aatcgtgatc tcgccgaaga gctcatcctg gtcgacgtcg acgacctgcc
     3181 ggcgggcaag gacgagaact gccaggaagg ccgtgcgcgc ctcatgcaga tcctcgcaat
     3241 gccgggtaat cgaccgaaac gaggcctggc cagaatgttg aagcccggcc agaacccgcg
     3301 ccgccatgtc gcgcagggag actagcttgc gcgcggcaac gatcaggctc ggcgccggga
     3361 cgacagagag gcgacgacgc agcgcacggg cgagctgcag cggctggtgc gcggcgagcg
     3421 gtcgcagaac ggtccgatcg tcggccggga gcggagcgcg cgcgtgtgga aaggcgccgg
     3481 acccgcgcgc atcgcgctct ttgagcagcg tcgccatctc cttcaccgcc cggtactcga
     3541 tcaattcgcg ggtgaggtcg ctgacctgct cgacctcttc gaccggcgga cggggcagga
     3601 gagaccggct cttgagcacg acgagccggc tcccaaccga tgagaactcg gcgatcagct
     3661 ccgcgtcgga ttgatccagg gtctcgacgt acgcgaggaa ctgcgatgtc accgcgacga
     3721 gcgagacgtc ggtgatcgcg agatggcttc gctcgaccag ccgcaagagc acgtcgaacg
     3781 ggccctcaaa cgctggcagc cgaagctgat acccctggaa gagagcggac tccaccgcaa
     3841 cgctgttcac gatagtcaca gtgtacggga aaacggtttg gtcttgcaga ttgattcgca
     3901 gggtttacgg tgtcgccgag tcaatttcct gaagcaaacg gactgccgga tcaggatgcg
     3961 tgcagtcgtc gtgccgttcg acgatctcgc aaatgcctgg ttccacggcg agggcgcgca
     4021 atcggtccgc acccaggtgg ccgtggttct tggcgagccg caagccgacc ccgagccatg
     4081 gtccggatcg ttcgatcatg cgcgcttcga gcgacggcga gaagtgctgg agcaggacat
     4141 gggcgatccg cgcggccagg ctgatcctgg tgccggccaa tgtcaccttg ccgatatcgt
     4201 gtagcaatcc ggcagtgcag acatcgggcg ggagccctgc ggcaactgcc ttctcgtaca
     4261 cggcgatcag gtgacgctgg tcgtgcggat gcagcccttg gaacaaccgg cacacgtgct
     4321 cgtttggcaa gagcgtgggg tccacgtcgg gcgctgttcg gagcgtgagg gccagccaga
     4381 tctggtgcag gcggtgaatg accgggtgat gggtggggga cgtcagatga aggaggtcaa
     4441 tcaccccagg agccagcgga cgagatcaag cagcagtctg tagccagggc cgatcagttc
     4501 gttgtagatg cccagattca gccgcccgtc gatgaagatc aggacgaacg ggaggaacag
     4561 gccgaattgc tcgagcttgg cgaaggtcgg ataccagaag tttggcagga tggcggtcag
     4621 cacccggtaa ccatcaagcg gtggcagggg aatcaggttg aaagccgcca gcaggatatt
     4681 aagcgtgatg aatgcaatca gaaaggtgcc ggtaaatccg agatcccgat tctggccgtc
     4741 aagtccgcgg aagatcaatg tcgcgcagca ggccatcacg acgttggaga gcggcgcaca
     4801 tccggcgatg accagacttt cgagcttgcg ctgcctgaaa tctccgcgga gccggttgaa
     4861 gttgaccgga accggcttgc cccacgcgat cgaggtgaat ccgaggctga tcaggacgaa
     4921 tccgaagaac ccgagtggat cgaagtgatt cgccgggttc aggttgaccc ggttcatcat
     4981 cagcgcggtg tcgtcgccaa acatgtgcgc cgaccacgcg tgcatgaact cgtgcacggt
     5041 gatcgcgatg atgccggaaa ggaggtatgc cgcaatctgg tcggagggcg gaatgttcca
     5101 gccaccctgc agcagattcg gcagtacgaa aatgagcagg ccgagaatga agagttgtct
     5161 cggatcccag cctgttcgca cggtttagcc tcgaaggtcg cctacttgcc ggaccccgga
     5221 tagagatggc aagcgacgaa atgcccgttg ccctgattcg cgaagaccgg atcgatctgc
     5281 ttgcagacgt ccatcacgta cgggcagcgg gtgtggaaat gacaaccgga gggtggatta
     5341 atcgggctcg gcacgtcgcc ggtcaggatg atgcgctcgc gccgcttctc aaccaccgga
     5401 tccgggatcg gcacggctga gagcagcgcc ttcgtatacg gatggagcgg ctgatcgtaa
     5461 agctcgttgc ggctggccat ctcgacgatc ttgccgaggt acatcaccgc gacgcgatcc
     5521 gagatgtgct tcacgaccga gaggtcgtgg gcgatgaaca ggtaggtgag gccgaactgg
     5581 tcctgcagat cctcgagcag gttgacgatc tgcgcctgga tcgatacgtc cagcgccgaa
     5641 accggctcat cggcgacgat gaagtccgga ttggccgcga gcgcccgggc gataccgatg
     5701 cgctgccgct ggccgccgga gaactcgtgc ggataccgat tggcgaagta cgggttgaga
     5761 ccgaccgtct ggagcaggtc ctggacgcgc ttgttgcggt ccttcttgtc gaccagcccg
     5821 tggatctgca tcggctcggc gatgatgctg ccgaccgtca ttcgcgggtt gagtgacgca
     5881 tacgggtcct ggaagatcat ctgtaggtgg cgccgcatct tgcgcatttc gccgcccggc
     5941 agcttggtca ggtccttgcc gcggaagacg atttcgccgc tggtcggctt gtagagctga
     6001 aggatcgcgc ggccggtcgt ggatttgccg cagccggact cgcccacgag gccgagggtc
     6061 tcgcccttct tgacctcaaa cgagatgccg tcgacagcct ggacagcgcc aaccttgcgc
     6121 tggaagatga tgccctgcgt cagcgggaag tgcatgacca ggttatcgac tttcaggagg
     6181 gcgtcgcccg agccgttcgg cgttgtcgaa atcggatcgg ccatggtcta cgcatccctc
     6241 cggggcggtt cattgtcctt gttgacttcg tcccagaacc agcacgctga gtaatggccg
     6301 ggcgcgactt cgaggagtgg cgggttcttc tgctcgcact tttcccgcgc atagttgcag
     6361 cgcgggacga acgggcacat atctggcaag tcgatcagat ccggtggaag tccgcggatc
     6421 ggctcgagtt tttccttggt ggtcgcatcc aggcgcggga tcgacttcat caggccgacc
     6481 gtgtacgggt ggcgcgggtt cgcgaagatc tcttcggtgg tcgccgtctc gacgatatgg
     6541 ccggcgtaca tgacctggac gcgatcggcc ataccagcca cgacgcccat cgagtgggtg
     6601 atcagaatca cgccagtgtc gcgctcgctt tgcagcgtcc gcatcaggtc caggatctgg
     6661 gcctggatcg tcacgtcgag cgcagtcgtc ggctcgtcgg cgaggatcag tttcgggttg
     6721 caggagagcg ccatggcgat catgacgcgc tggcgcatac caccggagaa ctggtgcggg
     6781 tactggtcaa gccgcttctc cgcgttcgga atcccgacca tcttgagcag gtcgaccgcg
     6841 cgggcgcgag cctgatcctt gttcaagcca aggtggagct gaatcgcctc gcagagctgt
     6901 cgactgatcg tcaataccgg gttgagcgag gtcatcgggt cctggaagat catggcgatg
     6961 tcgttgccgc gaatggaccg aacttgctcg tccgacatcc gcaagatgtc atcgccgtag
     7021 aacagaatct
//
LOCUS       DATAOZ010000557         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1004738, whole genome shotgun sequence.
ACCESSION   DATAOZ010000557 DATAOZ010000000
VERSION     DATAOZ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004738"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VHR64_14060"
     CDS             complement(<1..272)
                     /locus_tag="VHR64_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304007.1"
                     /translation="MTRHILMVVTLAVVLLGALSAPGAALARQATPAASPIPEQTAPL
                     GIGTVTLPEDRPAIVALFKTVMKTGTETRFAGMTTEEARITLTIGNP"
     gene            382..669
                     /locus_tag="VHR64_14065"
     CDS             382..669
                     /locus_tag="VHR64_14065"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEX3304008.1"
                     /translation="MVARRNRSEKLDLRLSREAKETIYAAAAAAHRTVSDFVVESALR
                     QAEETLADRRHFGLNAEQWTAFLEELDRPPRDLPQVEKLFREPSIFESGNS"
     gene            666..1175
                     /locus_tag="VHR64_14070"
     CDS             666..1175
                     /locus_tag="VHR64_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3304009.1"
                     /translation="MTSIRIEKLARQHGLETFDCGQAALNHFLIRFAFPNQQANASQT
                     YLGLAGSAVIGFYTLVVGEIAFEDAPERLAKGLARHPIPVMVLARMGVDQKWQGQGVG
                     AGLLKDAMRRTVQAADIAGIRAFVVHAKDGRARAFYEHYGFIPSSSDPLHLHMLVKDL
                     RRVIGEPRG"
     gene            1238..1600
                     /locus_tag="VHR64_14075"
     CDS             1238..1600
                     /locus_tag="VHR64_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304010.1"
                     /translation="MRRIWLAAIAIALLVSVLGVALLQHNNGAQAQATKTPVLVMEVA
                     SWAKGDVDPTAWQWQALESGALHVAFWSPGKMACAEIALPPGVTATYANAKGKTATVA
                     GPGDAVEMCEALFSRANG"
     gene            complement(1603..>3277)
                     /locus_tag="VHR64_14080"
     CDS             complement(1603..>3277)
                     /locus_tag="VHR64_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX3304011.1"
                     /translation="VPTNTATATPEPTATSTATTIPTAAATATEFDENDLPWTIDVPV
                     DNLTATPAPVATETAVSEQDEDYGLPTAISDAVNNLTPTPPTSGAEVAVAEAATVAPK
                     PASTPSPRPTSTPVATAIPNVTAPDTPTAPATATLEPTAVPTVLSEIERVKNGSFEQG
                     VEGWYLESGAGSVPVADAPDGAAMLQLPATGGYADQVLFTIPGTSYYLSASGRLTAEG
                     DSGVVGVVYRDAAGTRLTDLEPKPISFTATKLRKKGLEFTPPEQVAKVSVYIYKDDGP
                     AALDVDAISVRSIVPPVAASDAPGGGLALAAGAQTILIMGVDARPGEAIDGQVRPDSL
                     MVVHLNPTSSSCRVLSIPRDTRTELPGYGLTKINHALALGGIDYEVQVVSDLIDLPID
                     HYVLIDFTGFEDLVDAVGGITIDVPEGFTAADGTVFPTGPQQMTGKQALSYSRHRGDA
                     EGDFGRIKRQQQVIRALIKQSSGLEVLASIREFLPAIQNNLRTDLNVPEMIDLASTYR
                     SICTEDAVTMMRLEGEIATFDDPILHMPLSYVVVDEAEIRRKVAALLEP"
BASE COUNT          543 a          979 c         1135 g          620 t
ORIGIN      
        1 gggttgccga tggtgagggt gatccgcgcc tcctcggtcg tcatcccggc gaaacgggtt
       61 tcggtgccgg ttttcatgac ggtcttgaag agggcgacga tcgccgggcg gtcctcaggc
      121 agcgtcacgg tgccgatccc aagcggcgcg gtctgctccg ggatcggcga ggccgccggc
      181 gtcgcctgcc gggcgagggc cgcacccgga gcgctgagcg cgccaagcag gaccacagcc
      241 aaggtgacca ccatcagaat atgccgtgtc atgacccctc cgccgaatgc aatccagcaa
      301 ttatggccga ccgccggacc aatgtagatg tcatgacaat gtccggcgat aatgatagag
      361 ttgtgcgtgg aggtttgtat tgtggttgca cgacgaaatc gatctgaaaa attagatctg
      421 cggctatcgc gtgaggcgaa ggaaaccatc tatgcggctg cggccgctgc gcatcgtacc
      481 gtgagcgatt ttgtggttga aagcgccctg cgccaggcgg aggaaacgct cgctgatcgc
      541 cgccactttg gcctcaatgc cgaacaatgg acggcatttc tcgaggaact cgaccggcca
      601 ccccgtgatt tgccccaggt tgaaaaactc ttcagggagc caagcatctt cgagtccggc
      661 aactcgtgac gtcgatccga atcgaaaagc ttgcccgaca gcacggtctc gagacattcg
      721 actgtggtca agcggcgctc aaccatttcc tcatccggtt cgccttcccg aatcagcaag
      781 ccaacgcctc tcagacctat ctgggtctcg ctggttcggc ggtaatcggc ttctacacat
      841 tggtagtcgg agagatcgca ttcgaggatg ccccggagcg cttggccaag ggcctcgcgc
      901 gtcatccgat cccggtcatg gtgcttgccc ggatgggcgt tgaccagaaa tggcagggcc
      961 agggcgtcgg agcagggctc ttaaaggatg caatgcgtcg taccgtacag gcggccgaca
     1021 tcgccgggat tcgcgcgttc gttgtgcacg ccaaggatgg gcgcgcccga gcattctacg
     1081 agcattacgg ctttattccc tcttcatccg atccactcca tctccatatg ctcgtcaagg
     1141 atttacgaag ggtcatcggc gaaccccgag gctgacggtt gcagcgacgg ggtatggttt
     1201 tgcgtcggtt gttggttggc tggaatgcag gtgtttgatg aggcgaattt ggctggcggc
     1261 gattgcgatc gcgttgttgg tatcggtttt gggggtcgcg ctcctgcagc acaataatgg
     1321 cgcacaggca caggcgacga agacaccggt cctggtcatg gaagtcgcct cgtgggcgaa
     1381 gggcgatgtc gatccgactg cctggcagtg gcaggcgctc gagagcggcg cgctgcacgt
     1441 cgccttctgg tcgcccggca agatggcctg cgccgagatc gcgctgccgc ccggagtgac
     1501 cgcaacctat gccaacgcca agggaaagac agcgacggtc gccgggccgg gggatgcggt
     1561 cgagatgtgc gaggcgctct tctcccgcgc caacggatag ggctacggct cgaggagcgc
     1621 ggcgaccttg cggcggatct cggcttcgtc gaccacgacg tagctgagcg gcatatgcaa
     1681 gatcggatcg tcgaaggtgg cgatctcgcc ctcgaggcgc atcatcgtga ccgcgtcttc
     1741 ggtgcagatc gagcggtagg tgctggccag gtcgatcatc tccggcacgt tcaggtcggt
     1801 gcgcaggttg ttctggatag ccggcaggaa ctcgcggatc gatgcgagca cctcgagccc
     1861 gctcgattgc ttgatcagcg cccggatcac ctgctgctgt cgcttgatcc ggccgaaatc
     1921 gccctcggca tcgccccggt ggcgggagta ggagagcgcc tgcttgccgg tcatctgctg
     1981 cgggccggtc gggaagacgg tgccatcggc ggcggtgaaa ccttccggca cgtcgatcgt
     2041 gatcccgccg accgcgtcga ccagatcctc gaaaccggtg aagtcgatca gcacgtagtg
     2101 atcgatcggc aggtcgatca ggtcgctgac gacctgcact tcgtaatcga tcccgccgag
     2161 ggccagcgcg tggttgattt tggtcaggcc gtagccgggc agctcggtgc gagtgtcgcg
     2221 cggaatcgag aggacgcggc aggagctcga ggtcgggttg aggtggacga ccatcagcga
     2281 atccggccgg acctgcccat cgatcgcctc gccggggcgg gcatcgacac ccatgatcag
     2341 gatcgtctgc gcgccggcag ccagggccag tccaccgccg ggcgcatcgc tcgcggcgac
     2401 cggcgggacg atgctgcgaa cagagatcgc gtccacgtcg agtgcagccg ggccgtcgtc
     2461 cttatagatg tagacgctga ccttggccac ctgctccggc ggcgtgaact ccagcccctt
     2521 tttcctgagc ttggtcgcgg tgaagctgat cggtttcggc tccagatcgg tcagccgggt
     2581 cccggcggca tcgcggtaga cgacaccgac tacgccggag tcaccctcag cggtcaggcg
     2641 accactcgct gagaggtaat aggaggtgcc cgggatcgtg aagaggacct gatcggcgta
     2701 gccgccggtt gccgggagct ggagcatcgc ggcaccatcc ggcgcgtcgg cgaccgggac
     2761 cgatccagcg cctgattcga ggtaccagcc ctcgacgcct tgctcgaagc tgccgttctt
     2821 gacccgctcg atctcggaga ggacggtggg cacggcggtc ggctcaagcg tggccgtggc
     2881 gggcgccgtc ggggtatcgg gcgccgtcac gttcgggatt gcggtggcca cgggcgtact
     2941 ggtcgggcgc ggcgatggag tgctggcggg cttgggcgcg acggttgcgg cctcggccac
     3001 tgccacttcg gcgccgctcg tgggtggtgt cggtgtcaga ttgttgacgg cgtcgctgat
     3061 cgcggtcggc aggccgtaat cctcgtcctg ctcggagacg gccgtctcgg tcgcgaccgg
     3121 tgccggcgtg gcggtcaggt tgtcaaccgg gacatcgatc gtccagggca ggtcgttctc
     3181 gtcgaactcg gtcgcggtgg ccgccgcggt cggtatcgtc gttgcggtgg atgtggccgt
     3241 tggctcgggg gttgcggtcg ccgtattggt cggaacc
//
LOCUS       DATAOZ010000558         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1268450, whole genome shotgun sequence.
ACCESSION   DATAOZ010000558 DATAOZ010000000
VERSION     DATAOZ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268450"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VHR64_14085"
     CDS             complement(<1..364)
                     /locus_tag="VHR64_14085"
                     /inference="COORDINATES: protein motif:HMM:NF040428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HEX3304012.1"
                     /translation="MAQKVHGRLLEERAIDCERGSKSKRGQRPARSVTVNLTESPLGW
                     LFSRDMVTRRQFDAGERLRADWERAQLPARVTMAWDAAPVSGRRGGGSASADLTGSQI
                     DAKRRFQDAVDAAGPGLAD"
     gene            454..1017
                     /locus_tag="VHR64_14090"
     CDS             454..1017
                     /locus_tag="VHR64_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX3304013.1"
                     /translation="MITRIREVRRARGMTLDDVAKACKPQTTPQTIGRLETGTRTVSI
                     GWLNRIADALGVDAADLVQGGGDTAELPVVALLGTGGATAPKRTATVVAPRPQGGQVA
                     ITVTSSNGDYRAGDEIWCDMIEGDFARALNRDVLVPRPAGRFLFGRLIDRDEERLQLL
                     PLGSGGRQQIVANPPWIAVTKKLVRNL"
     gene            complement(1143..>1798)
                     /locus_tag="VHR64_14095"
     CDS             complement(1143..>1798)
                     /locus_tag="VHR64_14095"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX3304014.1"
                     /translation="DRTNHKESTGHGSPLPVLVHGGPGRAGGGEEMGGIRGVKHYMQR
                     TALQSSPTMIAAVTEQYVPGAPKHIIDVHPFRLEMSELNIGDTWKTPARTVTIEDIEQ
                     FAEFTGDKFYAHMDDEAAKASPIFEGRVAHGYLILSFAAGLFVDPDPGPVLANTGLEN
                     LRFLTPLYPGDSLRVELSVRSKSIKSEETGQVRWAVEVYNQKDELVATYDLLTENVP"
BASE COUNT          299 a          561 c          583 g          355 t
ORIGIN      
        1 tgtctgcgag gccggggccc gccgcatcga cggcgtcctg gaatcgtcgc ttcgcgtcga
       61 tttggctgcc cgtgaggtcg gccgatgccg aaccgcctcc gcgccggcca ctgacgggcg
      121 ctgcgtccca agccatggtt acgcgagccg gaagttgcgc gcgctcccaa tcggctcgca
      181 gccgctcccc ggcgtcgaac tgccgacgcg tgaccatgtc tcgagaaaag agccagccca
      241 gcggcgattc cgtcaggttg accgtcaccg aacgagccgg tcgctggccc cttttcgact
      301 tcgagccgcg ttcgcaatcg atcgcgcgtt cctcgagcaa gcgtccgtgc actttctgag
      361 ccaagcctcg gcctcctttt tcgattcgcg acaggcttgc cacatcggca aatctgtagg
      421 acagagacaa accgaatggg ttagggggat ttaatgatta cgcggattcg ggaggtgcgt
      481 cgcgcgcggg gtatgacgct cgacgacgtg gcgaaggcat gcaagccgca gaccactccg
      541 cagacgatcg gccgcctgga gacgggcacc cggacggtat cgataggttg gctcaaccgc
      601 attgcggacg cacttggcgt cgatgctgcg gacttggttc aaggtggcgg ggacacggcg
      661 gaactgccgg tcgtcgctct tctcggtacc gggggcgcga ctgccccgaa gcgcacggca
      721 acggtcgtcg cgcctcggcc gcaaggcggg caggtggcga tcaccgtcac gtcgagcaac
      781 ggcgattatc gcgcgggtga tgaaatctgg tgcgatatga tcgagggcga ttttgcccgc
      841 gccctcaacc gggacgttct cgttccgcgc ccggccggga ggttcctgtt cggtcggctg
      901 atcgaccggg atgaagagcg cctgcaactt cttcccttgg gctccggcgg acgccagcag
      961 atcgtggcaa atccgccgtg gatcgccgta acgaagaagc tggttcggaa cctctagccg
     1021 cagccgtcat ttgcgacggg catgttcctg ttcttttcga accgcttggg ctgcggcatt
     1081 tcgctgctcg taacagtaat cgggacgctt ctgatcctct acctgctcgg ctggatcggc
     1141 cgttaaggca cgttctccgt cagcaggtcg taggtcgcga ccaattcatc cttctggttg
     1201 taaacttcga ctgcccagcg aacctgtccg gtttcttccg acttgatcga ctttgatcgt
     1261 acgctcagct ccacccggag ggaatcaccg gggtaaagcg gcgtcaggaa gcgcaggttc
     1321 tcgaggcccg tgttggcaag caccggaccc ggatcgggat cgacgaagag gcctgcggca
     1381 aagctcagga tcaggtagcc gtgagcgaca cggccttcga agatcgggga agctttggcc
     1441 gcttcatcgt ccatatgggc gtagaacttg tcgccggtga actccgcgaa ttgctcaatg
     1501 tcctcgatcg tgaccgtgcg agcaggcgtc ttccacgtgt cgccgatgtt gagctcgctc
     1561 atctctagcc ggaacgggtg cacgtcgatg atgtgtttgg gcgcccccgg cacatattgc
     1621 tcggtgaccg cggcgatcat cgtcggcgag gactggagcg ccgtgcgctg catgtaatgc
     1681 ttcacgccgc ggatgccgcc catctcctct ccgccccctg cgcgtccggg accgccgtgg
     1741 acgagcaccg ggagagggga cccgtggccg gtcgattcct tgtggttagt ccggtcga
//
LOCUS       DATAOZ010000559         7834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6007163, whole genome shotgun sequence.
ACCESSION   DATAOZ010000559 DATAOZ010000000
VERSION     DATAOZ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6007163"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VHR64_14100"
     CDS             <1..1261
                     /locus_tag="VHR64_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304015.1"
                     /translation="SSTVTGGTETPGRGTDRPRPKPIDPDVEELWPERQRNWRPERLR
                     ALAEAVEQRDLDRVDALWVELRDEPGAGALLPAIEALRQFQSGHHAVTAAEIRAKHAS
                     SRKSQIRRRFANALDHNDRAALADLALSGDIDEIDELSEASTRRIVGSLAIGHLERAL
                     ESDDDMLIMEAYDARVLGDQGLLTSVQRNRVDLAFDRYAWLKDVRSAIRKRDLAAIDR
                     LYAAMPDGAHQRLTEREHARIERLHAQESALRSLRAAISEGRDADAITALNAVERSGA
                     VIPQDLSWANVSDVIDRYSLMSSVRRAAEQHPRDIARLARLLPQLKEKFGGAFPETGD
                     GLDFDRLDAEITQSAQLVRIREALASNDDRKIVAAALPDVFGVLPMLERGEQARIERA
                     VAAANRALRRSGHRSSNTPSSATVDTV"
     gene            complement(1213..1866)
                     /locus_tag="VHR64_14105"
     CDS             complement(1213..1866)
                     /locus_tag="VHR64_14105"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX3304016.1"
                     /translation="MPSRAFDIALFDLDGTIADTLPLIYEAFDAAFIPALGSSFPPDR
                     IRAMFGPPDHEIIRRQVPESDADAAIERFNRHYEDRHDELVSAFPGLANLVRTCAAAG
                     MRLGVITGKSRLTALITLDRLGLTDAFGAIYAGDDVERQKPDPEALILALRDLGAAKS
                     DRAVMVGDAAADVVAGKSIGITTIGVTWGNPDHHELDESEPDYTVSTVAELGVLLLR"
     gene            1996..3369
                     /locus_tag="VHR64_14110"
     CDS             1996..3369
                     /locus_tag="VHR64_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX3304017.1"
                     /translation="MTLTAPRRNRVVADHYETDDQPIGTGGMALVYRGRDLRTRRDVA
                     LKTLKPEWVHDAHARARFRHEARTMAFLSHPNVARVYDLFEPNEQTQPWVVLEFIPGM
                     SLRQEIDRNGPMDIDRLAHLLKQIAAALNHLHQRGMVHLDVKPQNILFDDPMTVKLID
                     FGIAQEAGTVLEVNNGQAFGTVSYVSPEQASGEEVGPASDIYSLGCVVYEMVTGETLF
                     RYLDGTDPQIVLAAHLTEAPIPPTRRRPDLELPEWIDDIVLDALAKDPGDRYPTTVAF
                     AEAFESALNAATPPDSTVPLSRLPRFDPRANPTIVHTPPPVVGARIKGPPLVARIRTV
                     FLWKLVGIVALGNLLLAALSYWDTGVIPGLYDPTVAIRAGTTVTVTAELLNVRAAPSD
                     SGQVVDQVSQSAELRVTGLPDGGWWPITYDRDGEQMEGWVSAEHVEGVPQSGYARIRE
                     MVQDLTP"
     gene            3424..4131
                     /locus_tag="VHR64_14115"
     CDS             3424..4131
                     /locus_tag="VHR64_14115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3304018.1"
                     /translation="MTDESRDRSTGEDIAFALRVRQGDHDAFRALYERYGGAVLGLAQ
                     SVVHDRSAAEDITHDVFLGFWRNPYAFEPSRGQFVAWILRVTRNRAIDILRKRRDMSF
                     GTARTTQDGETIDPTQWLADPDPGPEAEAVTHTLADDVRAALRTLPEDHRRLLELAYF
                     GGLTQREIAERLHRPLGTVKTQMRTSLMKLSKLASVQALAQSAEEDQLVEEERGDPGS
                     WSLTSANPADITGPETS"
     gene            4146..4931
                     /locus_tag="VHR64_14120"
     CDS             4146..4931
                     /locus_tag="VHR64_14120"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX3304019.1"
                     /translation="MKFESTNNLCHEISDLIEGYAIGALDEREMLRVAESLPSCPEQQ
                     EQLREYEETIGILGLTAVPIAPPAALWERLNRSTVGEPLPEPISIRSRAAGKLAVPRW
                     MAAALAAAIVLLLLSSVSLGVALRQERSDNSTSFDSTMATYLTSGGQVVPLASLQTPD
                     QLGWAGKGSLLMAPNMPPVLVVDKCVPSSSHYAYFVWLQRGNQRTPMGNIEIGKDGQG
                     MMTFEGMNSLDSYDVVGVTIRTSNEAGQEWTYDVMTGSPRHTN"
     gene            complement(5468..6880)
                     /locus_tag="VHR64_14125"
     CDS             complement(5468..6880)
                     /locus_tag="VHR64_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3304020.1"
                     /translation="MATTVASSKSNPATADPTAPAYLNKEAMDHVWIHSVPWIELAER
                     NGLHVFDRGEGSTIYDVDGNAFLDGIAGLWVVNAGHGRKEIAEAMERQAARLAYVSSM
                     NFTTVPAVQLADMIAERTPGDLNRVFFAAGGSEAVESAIKIAKQVQAMRGFARRYKII
                     ARRGSYHGMTYGAMSLTALKNEQHFGPFMYGVSHVPHPNKYRSDFGVEGEQADIMAAR
                     AVEQEIMFQGPDTVAAVIGEPISTAAGVHVPSKIYWQMLREICDKHGVLLIADEVING
                     FGRTGTMFAIEQYGIVPDIMTVAKALSSGYAPISAAIIRDSVFEIFKEKQEVPFGHLL
                     TFGGHPVAAAAAIKNLEIFDEEKLVERGAETGAYLGKRLEELRSHPSVGDVRGTGMVW
                     ALDLVKSKKDKTVWGRDHGFIRKVQDVSMANGVITRTWDVMHFAPPLVITKEEVDRMV
                     AVADEALTAAEKEFAGEITD"
     gene            7046..7366
                     /locus_tag="VHR64_14130"
     CDS             7046..7366
                     /locus_tag="VHR64_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3304021.1"
                     /translation="MNAHGARRWATGTASAVQITFDGEPLSALETDTVAGALIAAGHR
                     VFTIDPHDGSPRGGFCFVGRCSDCLMVIDGQPGTLACVTTVRDGMRVETQQGHGHWDG
                     EGQP"
     gene            7363..>7834
                     /locus_tag="VHR64_14135"
     CDS             7363..>7834
                     /locus_tag="VHR64_14135"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3304022.1"
                     /translation="MSIAVIGAGPAGLIATALLAKRGESVTLIDEQAEAGGHLRYDRY
                     PVGSDRHQSDSWLTELGSAVYSSGATMLQSAIIWAAFRVANGLELAVNHAGAEQTVRA
                     DHLVVATGTTDLPLVIPGATLPGVMTSRAVRILLNRHGVLPGQRLVIAGTGSEAD"
BASE COUNT         1445 a         2420 c         2543 g         1426 t
ORIGIN      
        1 ctcgagcacc gtcaccggtg gcacggagac tcccgggcgg gggaccgatc gaccccggcc
       61 gaaaccgatc gatcctgatg tcgaggaact ctggccggag cgacagcgca actggcggcc
      121 ggagcgactg agagcgcttg ccgaggccgt cgagcagcgc gacctcgatc gggtcgacgc
      181 gctttgggtc gaacttcggg acgaaccggg cgccggggca ctcttgccgg cgatcgaggc
      241 acttcggcag tttcaatcgg gccaccacgc agtgacggct gcggaaatcc gcgcgaaaca
      301 cgcgagcagc cgcaaatcgc agattagacg gcgcttcgca aacgcgctgg atcacaacga
      361 tcgggcggcg ctggcagatc tcgcgctgtc gggtgacatc gacgagatcg atgagctttc
      421 cgaggcgagc acgcgccgga ttgtcgggtc tctggcgatc gggcatctcg aacgcgccct
      481 cgagagcgac gacgacatgc tgatcatgga ggcgtacgat gcgcgcgtcc tgggagatca
      541 gggactcctt acgtcggtcc agcggaatcg ggtcgacctc gccttcgatc gttatgcctg
      601 gctcaaagat gtccgctccg ccattcggaa gcgggacctc gccgctattg atcggctcta
      661 tgccgcaatg cccgatggcg cgcaccagcg actgacggag cgggaacatg cgcggatcga
      721 gcggttgcac gcgcaggagt cggcgctccg ctcacttcgc gcggcgatct ccgagggacg
      781 ggacgccgat gcgatcacgg ccctgaatgc ggtcgagcga agcggcgcgg tgatcccgca
      841 ggacctgtcg tgggccaacg tctcggacgt gattgaccgg tactcgctga tgtcctcggt
      901 tcgccgcgca gccgagcaac acccgcgcga catcgcccgt ctggcgcgac tgctgccgca
      961 gctcaaggag aaattcggcg gtgccttccc ggagaccggg gatggtctgg attttgacag
     1021 gctcgacgcc gagattaccc agagcgcgca gcttgttcgc atccgggaag cactcgcatc
     1081 caacgatgac cgcaaaatcg tcgccgccgc gctgccagac gtcttcggtg tcctgccgat
     1141 gctcgagcgc ggcgagcagg caaggattga gcgggcagtg gcagccgcga accgtgccct
     1201 tcgccgctcg ggtcaccgca gcagcaacac tcccagttcg gcaaccgtcg acacggtgta
     1261 gtcaggttcg gactcatcca gctcgtggtg gtcggggttg ccccaggtga cgccgatcgt
     1321 ggtgatgccg atgctcttgc cggcgacaac atcggccgcc gcatcgccga ccatgaccgc
     1381 gcgatctgat ttcgccgcgc cgagatcacg caacgccagg atcagcgctt ccggatcagg
     1441 cttctgccgc tcgacatcgt cgccggcata aatcgccccg aaggcatcgg tcagcccgag
     1501 acgatcgagc gtgatcaggg ctgtcagccg gctcttaccg gtgatgacgc cgagccgcat
     1561 acctgccgcc gcgcaggtcc ggacaagatt cgcaagtccc gggaatgccg acacaagctc
     1621 gtcgtgtcga tcttcgtagt gccggttaaa tcgctcgatt gcggcgtcgg cgtcggattc
     1681 aggtacctgg cgacggatga tctcgtggtc cggcgggccg aacatggccc ggatcctgtc
     1741 aggcggaaaa ctgctcccga gcgcggggat gaaggcggcg tcgaaggcct cataaataag
     1801 cggcaatgtg tcggcgatag tgccgtcgag gtcgaagagt gcgatgtcga aagcgcggga
     1861 cggcatgtct ctcccagctg ttcataatgt gcggacgacg cgtttgttag agtatgcagg
     1921 agttccgggc cgcacggtct tcttccggca tcaatcgact ctggcaatcc tagacgcgga
     1981 catgtgagac ctcgtatgac cttgaccgct ccgagacgca accgggtggt cgccgaccac
     2041 tatgagacag acgatcagcc gatcggaacg ggcggaatgg cgctggtcta ccggggacgg
     2101 gaccttcgca ctcgccgcga cgtcgccctc aagacgctca agccggaatg ggtgcatgat
     2161 gcccacgccc gggcccgctt ccgccatgag gcgcgaacga tggccttcct gtctcatccc
     2221 aatgttgcgc gggtttacga tcttttcgaa ccgaacgagc agacgcagcc ctgggtcgtg
     2281 ctggagttca tcccgggaat gtcgctcagg caagagatcg accgcaacgg gccgatggac
     2341 atcgatcgcc tggcgcacct gctcaagcag atcgccgctg cgctcaatca cctgcaccag
     2401 cggggcatgg tccacctcga cgtgaagccg caaaacatcc tgttcgatga cccaatgacg
     2461 gtcaagttga tcgacttcgg catcgcccag gaggcaggca cggtccttga ggtcaacaat
     2521 ggccaggcat tcgggacggt atcgtacgtc tctccggagc aggcatccgg cgaggaggtc
     2581 gggccggcca gcgacatcta ctcgcttggc tgcgtggtct atgagatggt caccggcgag
     2641 acgctgttca ggtacctgga tggaaccgat ccgcaaatcg tgctcgcggc gcacctcacc
     2701 gaagcaccga tcccaccgac gcggcgtcgg cccgacctcg aactgcccga atggatcgac
     2761 gatattgtcc tcgacgcgtt ggccaaggat ccgggggatc gctatccgac gactgtggca
     2821 ttcgcggagg cgttcgagag cgcgctgaat gccgccacgc cgcctgattc gacggtgccg
     2881 ctgagccggt tgccgcgctt cgaccctcgc gcgaacccga cgattgtcca caccccgccg
     2941 cctgtggttg gggcacggat caagggaccg ccgctggttg cgcggattcg gaccgtcttc
     3001 ctctggaagc tggtcggtat cgtcgcgctc ggcaacctcc tcctggcggc gctttcctat
     3061 tgggataccg gcgtaatccc ggggctttat gatccgaccg ttgcgattcg cgccgggacg
     3121 acggtcacag tcacggccga gttgctgaac gtccgcgccg cgccgagcga ttccggtcag
     3181 gtagtcgatc aggtcagtca atcagcagag ttacgtgtca cgggtttgcc cgatggcggt
     3241 tggtggccga taacgtatga tcgtgacgga gaacagatgg agggttgggt atcggcagaa
     3301 catgtcgagg gcgtccccca gtccggatat gcgcggattc gggagatggt tcaggatttg
     3361 acgccgtgaa cttcgacctg gttcgatggt aggggactca gcggcagggg tgcagggaac
     3421 acgttgaccg acgagagtcg cgaccgttca accggcgagg acatcgcatt cgcgctgcgc
     3481 gtgcgtcaag gcgaccacga tgcgtttcgc gcgctgtacg aacggtacgg cggtgcggtt
     3541 ctcgggcttg ctcaatcggt cgtacatgat cgttccgccg cggaagacat cacgcatgac
     3601 gtgttcctgg gattttggcg caacccctac gcgttcgagc cgagccgggg ccaattcgtg
     3661 gcctggatcc tgcgcgttac ccgcaaccgg gcaatcgata tcctgcgcaa gcggcgcgac
     3721 atgtcgttcg gtaccgccag gacgacgcag gacggcgaga cgattgatcc gacccaatgg
     3781 cttgccgatc cggatcccgg accagaggcc gaagcggtga cgcataccct ggccgacgat
     3841 gtcagagccg ctttacgcac gttgcccgag gatcaccgcc gattgctcga gcttgcctat
     3901 ttcggtggcc tgacgcagcg cgagatcgcc gagcgactgc atcgaccgct cgggaccgtc
     3961 aagacacaga tgcgcacatc cttgatgaag ttgagcaagt tggcgtccgt gcaggctctg
     4021 gcacaatccg cagaagagga ccaactcgta gaagaggaga gaggtgaccc cggaagctgg
     4081 agtctgacct cggcgaaccc tgccgacatt actggcccgg aaacctcctg agtacctgag
     4141 caactatgaa attcgaatcg accaacaacc tttgccacga gatcagcgac ctcatcgagg
     4201 ggtatgcgat tggggcatta gatgagcgcg agatgttgcg cgtggcggaa tcgctgccga
     4261 gctgcccgga gcagcaagag caacttcgcg aatacgaaga aaccataggg atcctcggtc
     4321 taaccgcagt cccgatcgca ccgccagcgg cgctgtggga gcggctgaac cgctccacgg
     4381 tcggtgaacc gctgcctgag ccgatctcga tccgctctcg cgcggcgggc aagttggctg
     4441 tgccgcgctg gatggccgcc gccttggccg cggcgattgt gctccttctc ctttcgagcg
     4501 taagtctcgg ggtcgccctg cggcaggagc ggagtgacaa ttcgacctcg ttcgactcga
     4561 cgatggccac ctatcttacg tccggcggcc aggtcgttcc gctggcgtcg ctgcagactc
     4621 cggaccagtt gggctgggcg gggaagggca gcctgctgat ggcgccgaac atgccgccgg
     4681 tgctggtcgt ggacaaatgc gtcccgagca gcagccacta cgcctatttc gtctggttgc
     4741 agcgcggcaa tcagcgcacg ccgatgggga atatcgagat cggcaaggat ggccagggca
     4801 tgatgacgtt cgaaggcatg aattcgctcg atagctatga cgtcgtgggc gtcacgattc
     4861 gcaccagcaa cgaggcgggc caggagtgga catacgacgt catgactggc tctccgcggc
     4921 acacgaatta ggacaactca ccataaaatg cggtataatt gccgatggcc gtgaggggta
     4981 aagggagaga ccgaccggct ccatattctt gggtagcgac cgaggttatc gttgacagac
     5041 caacatgttg catgtgccgc gtgccaacgc gagttcgtca tcactccgtc tgaacgcctc
     5101 ttctacgcag aacgcgggta ccaggaacca tcctattgcc ccgaatgtcg cgcccgccgg
     5161 cgcgccgagc gtcactccga actgatggcc gtcaccggcc atacccacgc aaaagtcagc
     5221 cttgggacat acggtggctt cgcgccgcct ggtccgcgcg gcgctgaggg ctcgagccga
     5281 ttcactgcca cgtgctcgtc gtgcggcggc gaggcggtcg ttccgtttga gccgcggccg
     5341 ggccgcccga tctactgccg gcgttgccac caggcacggc gcggtcgata gttcggacca
     5401 aatggcagag atgaaaaagg ccgggaagtt cgcttcccgg ccgttgtgct attccagcgt
     5461 ttgatcgtca gtcagtgatc tcgcccgcga attctttctc ggcagccgtc aatgcctcgt
     5521 cggccacggc caccatccgg tcgacctctt ccttcgtgat caccagcggc ggcgcgaaat
     5581 gcatgacgtc ccaggtgcgg gtgatcaccc cgttggccat gctgacgtcc tggaccttgc
     5641 gaatgaagcc gtgatcccgg ccccagaccg tcttgtcctt cttgctcttg accaggtcga
     5701 gcgcccagac catacccgtg ccgcgcacgt cgccgacgga cgggtgagag cgcagctctt
     5761 cgagccgctt gccgaggtat gcgcctgtct cggcgcctcg ctcgaccagc ttctcctcgt
     5821 cgaagatctc gaggttcttg attgcggccg ccgcggctac cgggtgtccg ccgaaggtca
     5881 gcaggtggcc gaacggcacc tcctgcttct ccttgaagat ctcgaagacg gagtcccgga
     5941 tgatggcggc cgagatcggc gcgtatccgg aagagagcgc cttggcgacg gtcatgatgt
     6001 ccggaacgat gccgtactgc tcgatcgcga acatcgtacc ggtgcggccg aagccgttga
     6061 taacctcgtc cgcgatcagc agcacgccgt gcttgtcgca gatctcgcgc agcatctgcc
     6121 agtagatttt cgagggcaca tgtacgccgg ccgcggtcga gatcggctca ccgatcacgg
     6181 cagcgacagt atccggcccc tggaacatga tttcctgctc gacagccctg gcagccatga
     6241 tatcggcctg ctcgccctcg acgccgaaat cggatcggta cttgttcgga tgcgggacgt
     6301 gcgagacgcc atacatgaac gggccgaagt gttgctcgtt cttgagggcc gtcagactca
     6361 tcgcgccgta ggtcatgccg tggtacgagc cgcggcgagc gatgatcttg tagcggcgag
     6421 cgaaaccgcg catcgcctga acctgcttgg cgattttgat ggccgactcg acggcctccg
     6481 agccacccgc cgcgaagaag acgcggttga gatcgccagg cgtccgctcc gcgatcatgt
     6541 cggcaagctg gaccgcagga actgtcgtga agttcatcga ggagacgtag gccaggcgag
     6601 cagcctgccg ctccatggcc tcggcaatct ccttgcggcc atgaccggca ttaaccaccc
     6661 agagtccggc gattccgtcg agaaaggcgt tgccatcgac atcgtagatg gtgctgcctt
     6721 cgccacgatc gaagacgtgg agaccattgc gctcggccag ctcgatccac ggcaccgagt
     6781 ggatccagac gtgatccatg gcctccttgt tcaggtaggc gggtgcagtg ggatcggcgg
     6841 tcgccggatt cgatttggac gatgcgactg tggttgccat gcagggtgct ctcctcgagg
     6901 cgtgatcgat caacccgaca agcttcggcg gattgatgtg aaactgcatg agtatacctg
     6961 agccattccc aagctggttt gacgctcacc ggtcggaatg acaatatgac gccccagttg
     7021 gtcgcatgta ccgggatagt tgaggatgaa tgcacatggg gcccggagat gggccacggg
     7081 gacggcgagt gcggtccaga tcacgttcga cggcgagcca ctttccgcgc tcgagacaga
     7141 cacggtggcc ggcgcgctga ttgcggcggg acaccgcgtt ttcactattg acccgcacga
     7201 cggttcgcct cgtggcggat tctgcttcgt cggtcgctgc agcgattgcc tgatggtgat
     7261 cgacggccag cccggcacgc tggcctgcgt cacgactgtc cgcgatggaa tgcgtgtcga
     7321 gacccagcaa ggccatggtc actgggacgg cgaggggcag ccatgagcat tgcggtcatt
     7381 ggcgctggac cagcgggttt gattgcgacc gcgctgttag cgaagcgggg cgagtcggtc
     7441 acgctgatcg acgagcaggc ggaggcaggc ggccacctca gatacgaccg atatccagtc
     7501 gggtcagatc ggcaccagtc ggatagctgg ctgaccgagt tgggaagcgc cgtctactca
     7561 tcaggcgcga ccatgcttca atcggcaatt atctgggcgg cgttccgggt ggctaatggc
     7621 cttgagttag cggtcaatca cgccggcgcc gagcagaccg tgcgtgctga ccacctggtc
     7681 gtcgcgacag gcacgaccga tctgccgctg gtcatccccg gcgcaaccct gccaggcgtc
     7741 atgacgagcc gggcagttcg catcctgctc aatcggcacg gtgtgcttcc cgggcagcga
     7801 ctggtcattg ccggtacggg gtctgaggct gatc
//
LOCUS       DATAOZ010000560         6288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6268208, whole genome shotgun sequence.
ACCESSION   DATAOZ010000560 DATAOZ010000000
VERSION     DATAOZ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6268208"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VHR64_14140"
     CDS             complement(<1..693)
                     /locus_tag="VHR64_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX3304023.1"
                     /translation="MTNQPRTLADKVWEQHVVRSAPGEPDLLYIDLHLIHEVTSPQAF
                     DGLRMAGRPVRRPDLTIATADHNVPTLNINLPIVDPISAKQVAMLSQNCAEFGIRLHP
                     MGDPGQGIVHVIGPEFGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQT
                     LPQVRPNTMSIRVDGDLPAGSTAKDIILAIIGKIGTGGGIGSIIEYRGSTIQALSMEG
                     RMTICNMSIEAGA"
     gene            complement(713..2287)
                     /locus_tag="VHR64_14145"
     CDS             complement(713..2287)
                     /locus_tag="VHR64_14145"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX3304024.1"
                     /translation="MSSSESEVGNRIWVFDTTLRDGEQSPGATMTVEEKLEMADALVA
                     LGVDIIEAGFPAASPGDFEAVRMIANRAKGAVIAGLARANENDIERAATAVKDADQSR
                     VHTFIATSDIHLEHKLRMTREQVLERAAKMVAFAKSFTSDVEFSAEDATRSDWDFLVQ
                     VFGAAIKAGATTLNVPDTVGYTTQHEYRSLIEHLRANVEGIDDVVISVHCHNDLGMAT
                     ANTLAAIAAGAQQIEVTVNGIGERAGNTALEEVVMTLATRGEQYGGAFTNIKKDRIVP
                     TSRMLTNLTGLQVQVNKAIVGGNAFAHEAGIHQDGMLKNRTTYEIMNPQDVGWEGTKL
                     IVGKHSGRAGFKNALQELGIKLDDEQLQAAYLRFLELADRKKHVTSADLEALVTNQLE
                     TEGDPIQLVQWKVDIASGEPATAMVVLARSGQQIVGEADGNGPVEAILRAVDAATGLD
                     PELEYFNVEAIGPQKDAQGTVNLRIRHEGKLYTGHGLAPDIVEASARAYVAALSRLEQ
                     KKPSTVIAGSSTSRWT"
     gene            complement(2315..3337)
                     /gene="ilvC"
                     /locus_tag="VHR64_14150"
     CDS             complement(2315..3337)
                     /gene="ilvC"
                     /locus_tag="VHR64_14150"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX3304025.1"
                     /translation="MAATLYYDKDASLDPLVGKTIAIVGYGSQGHAHAQNLRDSGMKV
                     IVGLAEGSKSRAKAEADGFEVFSVADAAKKADLIMILAPDQVQGKIYEQSVKDGLTAG
                     KTLLFAHGFSVRFGFVSPPKDVNVVMVAPKAPGHRFRELFQEGAGVPGLVAVEQDATG
                     NAKEIALAYAKGLGLTRAGVIETTFKEECETDLFGEQAVLCGGITELIQAGFQTLVRA
                     GYQPEIAYFECLHEVKLIVDQIYENGISYMLYSVSDTAEYGSYYAGPKVIDAHVRQSM
                     QELLDRVQDGTFAKQWMAENESGREHFLQMRADSQNSQIEQVGKGLRAMMPWLHKGKT
                     PVDISA"
     gene            complement(3432..3995)
                     /gene="ilvN"
                     /locus_tag="VHR64_14155"
     CDS             complement(3432..3995)
                     /gene="ilvN"
                     /locus_tag="VHR64_14155"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX3304026.1"
                     /translation="MESARPKPRRHTLVVLVEDHPGVLNRVVSLLRRRSFNIHTITVG
                     ASEQPGVSRMTLVVEGDAQDVEQAGKQLYKLMEVLKVTDITDTNSISHELVLVKVATK
                     AQNRQEVMLAGQIYDAKFVDATPSSLIMEVTGSPERIDSFLHMVKPYGVRELVRTGPV
                     AMSRGGGTVQIRDGRIERANGVPAAAD"
     gene            complement(4001..5770)
                     /gene="ilvB"
                     /locus_tag="VHR64_14160"
     CDS             complement(4001..5770)
                     /gene="ilvB"
                     /locus_tag="VHR64_14160"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX3304027.1"
                     /translation="MAVANSGELLTRRPRRSEQRQLRTVAELEGVRMTGAQITCAALE
                     AEGADLVFGYPGGAVIPLYDAIPSSNLHHVLVRFEQWAALAADGYARATGKVGVCIAT
                     SGPGATNLVTGLANSQLDSVPVVAITGQVGQALIGRDAFQETDITGITLPITKHNYLV
                     QSADEIAPTIKEAFYLARSGRPGPVLVDIPKNVFQESARFHMPGEINRRGYQPSLLPN
                     TRQIRLAGEMINAAKKPLIMAGHGIMISGAEQEFERFVERTGIPVIFTLLGQGSFPES
                     HELSLGLMGMHGHREVNMALKECDLLVNIGARFDDRATGKVSTFAPNARVVHCDIDPA
                     EIGKNVSTDVPVVGDAREVLKLMLGEVNERNHGDWLAWIKAQKDVALRAALNDRPEWP
                     EPYTIIKEIARVTNGEAIYCTDVGQHQMWAAQHLGLDHPRRWLTSGGLGTMGYGLPAA
                     IGAKMGMPESEVWTIIGDGGFQMSSNELATCVQEQLDINIAVINNGYLGMVRQWQDLF
                     HQKNYSEVEILGPDFIKLAEAYGATGIRVTRDDEIVPAIEKARSIPGPVLIEFVVEPE
                     ANVWPIVPPGASNSDMMLNRGEE"
     gene            complement(6085..>6288)
                     /locus_tag="VHR64_14165"
     CDS             complement(6085..>6288)
                     /locus_tag="VHR64_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX3304028.1"
                     /translation="GQTPHTILADWASSDILNPGGTFEHTFDRAGTFEYQCGLHPAMV
                     GTVQVVEAAVASPEPSPSTPDIA"
BASE COUNT         1067 a         2065 c         1907 g         1249 t
ORIGIN      
        1 ggctcctgcc tcgatcgaca tgttgcagat cgtcatccgg ccctccatcg agagcgcctg
       61 gatggtcgaa ccgcggtact cgatgatcga gccaatgccg ccgccggtgc cgatcttgcc
      121 gatgatcgcc aggatgatgt ccttggcggt cgaaccggcc ggcaaatcgc cgtcgacgcg
      181 aatcgacatg gtgttcggcc ggacctgcgg caatgtctgg gtcgcgagga catgctccac
      241 ctcgctcgtg ccgatcccga atgccagcgc gccgaacgcg ccgtgggtcg atgtgtgcga
      301 atcgccgcag acgatcgtca tgccgggctg tgtcaggccg aactcaggac cgatcacgtg
      361 gacgatgcct tgccctggat cgcccatcgg atggaggcgg atcccaaact cggcacagtt
      421 ctgcgacagc attgcgacct gtttggcaga gatcgggtcg acgattggca gattgatgtt
      481 aagcgtcggc acgttgtgat cggccgtcgc gatggtcagg tctggccggc ggacgggtcg
      541 accggccatc cgaagcccgt cgaacgcctg cggcgacgtc acttcatgga tcaggtgcag
      601 atcgatgtaa agcagatccg gctcgccagg agcggagcgc acgacatgct gctcccagac
      661 cttgtcggcg agcgttctcg gttggttggt catcagtatt ctctgtcttc tcttatgtcc
      721 agcgtgatgt cgatgagccg gcgatcaccg tcgacggctt cttttgctcg agccggctga
      781 gcgcggcgac atacgctcgc gcgctagcct cgacgatatc cggtgccaat ccgtgaccgg
      841 tataaagctt gccctcatgg cgaatgcgga gattgaccgt cccctgcgcg tccttctgcg
      901 ggccgattgc ctcgacgttg aagtactcca gctcgggatc caggccagta gcggcatcga
      961 ccgcgcgcaa gatcgcctcg acgggtccgt ttccatcagc ttcgccgacg atctgctgtc
     1021 cgctccgcgc caggacgacc atcgcggtcg ccggctcgcc gctggcgatg tcgaccttcc
     1081 actggacgag ctgaatcgga tcaccttcgg tctcaagctg attggtcacc agcgcctcga
     1141 gatcggccga ggtcacgtgt ttctttcggt cagccagctc caggaagcgg agataggccg
     1201 cctggagctg ctcgtcgtcc agcttgatcc cgagttcctg caacgcattc ttgaacccgg
     1261 cccggccgga gtgcttgccg acgatcagct tggtaccctc ccagccgaca tcctgcggat
     1321 tcataatctc gtaggtcgtc cggttcttga gcatgccgtc ctggtggatg ccggcctcat
     1381 gcgcgaaggc attgccgccg acgatcgcct tgttgacctg aacctgcaat ccggtcaggt
     1441 tcgtcagcat ccggctggtc gggacgatcc gatccttctt gatattggtg aacgcacccc
     1501 catactgctc gccacgcgtt gccagcgtca tcacgacctc ttcgagcgcc gtgttacccg
     1561 cccgctcgcc gatgccgttg accgtgacct cgatctgctg cgcgccggcc gcgatcgccg
     1621 ccagtgtgtt cgcggtggcc atgccgaggt cattgtggca gtgcactgag atgacgacgt
     1681 cgtcgatccc ctcgacgttc gcgcgcaggt gctcgatcag cgaccggtac tcgtgctgcg
     1741 tcgtgtatcc aaccgtgtcc ggcacgttga gcgtcgtcgc gccggccttg atcgccgcgc
     1801 caaagacctg aaccaggaag tcccagtcgg aacgagtcgc atcctcggcc gagaactcga
     1861 catcactggt gaacgacttt gcgaaggcga ccatcttcgc cgcccgctcg aggacttgct
     1921 cgcgggtcat ccgcagcttg tgctcgagat ggatgtccga ggtcgcgatg aacgtgtgga
     1981 cgcgagattg gtctgcgtcc ttgacggcag tcgcggcgcg ctcgatgtcg ttctcgttcg
     2041 cacgggccag gccggcgatc accgcgccct tggcccggtt ggcgatcatc cggaccgcct
     2101 cgaagtcgcc gggcgatgcg gccgggaagc cggcttcgat gatgtccact ccgagagcaa
     2161 cgagggcatc ggccatttcg agcttctctt cgaccgtcat cgttgcgccg ggcgactgct
     2221 caccatcgcg caacgtggtg tcgaagaccc aaatgcggtt tcctacctca ctctccgaac
     2281 tggacatcac cgaactcctt ctaccgaact gaacctatgc cgaaatgtct accggggtct
     2341 ttcccttgtg cagccacggc atcatcgccc gcagcccctt gccgacctgc tcgatctggc
     2401 tgttctgcga atccgcccgc atctgcagga agtgctcgcg gccggactcg ttctcggcca
     2461 tccactgctt ggcgaatgtg ccgtcctgga cccggtcgag cagctcctgc atgctctggc
     2521 ggacatgcgc gtcaatcacc ttcggtccag cgtagtacga gccgtattcc gccgtgtcgc
     2581 tgacggagta gagcatgtac gagatgccgt tctcgtagat ctggtccacg atcagcttga
     2641 cctcgtggag acactcgaag taagcgatct ccggctgata tccggcccgg acaagtgtct
     2701 ggaagccggc ctggatcaac tccgtgatgc ctccgcagag gaccgcctgc tcgccgaaaa
     2761 gatcggtctc gcactcttcc ttgaacgtgg tctcgatcac gccggcgcgc gtcaagccca
     2821 accccttggc gtaggccagc gcgatctcct tggcgttgcc ggtcgcatcc tgttcgaccg
     2881 cgaccaggcc cggaacgcca gcgccctcct ggaagagctc ccggaagcgg tggcccggcg
     2941 ccttcggggc gaccatgacc acgttgacat ccttcggcgg cgagacgaag ccgaaccgca
     3001 ccgaaaagcc atgcgcgaag agcagcgtct tgccggcggt cagtccgtcc ttgaccgact
     3061 gctcgtaaat cttgccctgg acctggtccg gggcgaggat catgatcaag tcggccttct
     3121 tggcggcgtc ggcgaccgag aagacctcga agccgtccgc ctcggccttg gcccggctct
     3181 tgctgccctc ggccagaccg acaatcacct tcatgccgct gtcgcgcagg ttctgggcat
     3241 gcgcgtgacc ctggctgcca tagccgacga ttgcgatggt cttgcccacg agtgggtcaa
     3301 ggctggcgtc cttgtcgtag taaagcgtgg ctgccacacg agctcctttt cgatgaactg
     3361 gtcgccgatt gcgaccctct atccaaattc aaacggggag cggtggctcc ccgtctgcat
     3421 tctctcaaac ttcaatcggc cgcggcaggt acgccgttgg cccgttcaat ccgaccgtcg
     3481 cgaatctgga ccgtgccgcc gccgcgcgac atcgcgaccg gcccggtccg gactagctcg
     3541 cggacaccgt acggtttgac catgtgcagg aacgagtcga tccgctccgg cgagccggtc
     3601 acttccatga tcagcgacga cggcgtggca tcgacgaact tggcgtcgta gatctgccca
     3661 gccagcatga cttcctgccg gttttgcgcc ttggtcgcga ccttgaccag caccagctcg
     3721 tggctgatcg aattggtgtc ggtgatgtcg gtgaccttga gcacctccat cagcttgtag
     3781 agttgcttgc ccgcctgctc gacatcctgg gcatcgccct cgacgaccag cgtcatccgg
     3841 gagacgcccg gttgctcgga tgccccgaca gtgatcgtgt ggatgttgaa cgatcgccgc
     3901 ctgagcagcg agaccacccg gttcaggaca ccaggatgat cctcgaccag gacgacgagc
     3961 gtgtgcctgc gcggcttggg tcgtgcggat tccatgactg ttactcctcg ccccggttga
     4021 gcatcatgtc ggaattgctg gcgcccggcg gaacgatcgg ccagacattg gcctcaggct
     4081 cgactacgaa ctcgatcaga accgggccgg gaatcgatcg cgccttctcg atggccggaa
     4141 cgatctcgtc gtcgcgcgtg acgcgaatgc cggttgcgcc atacgcctcg gccagcttga
     4201 tgaagtcggg accaaggatt tcgacctcgg agtagttctt ctggtggaag agatcctgcc
     4261 actggcggac catgcccagg tacccgttgt tgatgaccgc gatgttgatg tcgagctgct
     4321 cctggacgca ggtcgccaac tcattcgatg acatctggaa tccgccgtcg ccgatgatcg
     4381 tccagacttc cgactccggc atgcccatct tcgcgccgat cgccgccggc agaccgtagc
     4441 ccatcgtgcc gagaccaccg gaggtgagcc agcgccgggg atgatcgaga cccaggtgct
     4501 gggccgccca catctggtgc tgcccgacat cggtgcagta gatcgcctcg ccgttcgtga
     4561 cacgcgcgat ctccttgatg atcgtgtacg gctccggcca ctccgggcgg tcgttcaggg
     4621 ccgcgcgcag ggcaacgtcc ttttgcgcct tgatccaggc cagccaatca ccgtggttgc
     4681 gctcgttgac ctcgcccagc atcagcttga gcacttcccg ggcgtcgccc acgaccggga
     4741 catcggtcga gacgtttttg ccgatttcgg ctgggtcgat gtcgcagtgg acgacgcggg
     4801 cgttcggcgc gaaggtcgag accttgccgg tcgcgcgatc atcgaagcgc gcgccgatgt
     4861 tgaccagcag gtcgcactcc ttgagcgcca tattgacttc gcgatgccca tgcataccca
     4921 tcaggccaag tgagagctca tgcgattccg ggaacgagcc ctggccgagc agcgtgaaga
     4981 tgaccgggat gccggtccgc tcgacgaacc gctcgaactc ctgctccgcg ccggagatca
     5041 tgatcccgtg accggccatg atcagcggct tcttggcggc gttgatcatc tcgccggcta
     5101 gtcgaatctg gcgcgtgttc ggcagcaggc tgggctggta gccgcgccgg ttgatctcgc
     5161 ccggcatgtg gaaccgggcg ctctcctgaa acacgttctt tgggatatcg acgagcaccg
     5221 gtcccggccg acccgaccgc gccaggtaga aggcttcctt tatcgtcggc gcgatctcgt
     5281 cggccgactg cacgaggtaa ttgtgcttgg tgatcggcag cgtgatgccg gtgatgtcgg
     5341 tctcctggaa ggcatcgcgc ccgatcaacg cttgaccgac ctgcccggtg atcgcgacaa
     5401 ccggaaccga gtcgagttgg ctgttggcca gaccggtgac caggttcgtc gcgcccggac
     5461 ccgaggtcgc gatgcaaacg ccgactttgc cggtcgcgcg ggcatagcca tcggccgcca
     5521 gcgctgccca ctgctcgaac cgcaccagca cgtgatgcag gttcgaagac ggaatggcgt
     5581 catacagcgg gattactgcc ccgcccggat agccgaatac cagatcggcg ccctcagcct
     5641 cgagcgcggc gcaggtaatc tgggcgccgg tcatgcggac gccctccaac tcggcaacgg
     5701 tccgcagttg cctctgctcg cttcggcgcg ggcgtcgtgt cagcagctcc cctgaatttg
     5761 cgactgccat ctggttctcc gttccctctc cgaccgttgt tcccgttcca gcccggaagg
     5821 cgacgcatca aaaaagcctc ccgatcgtgg gaggcctccg aactcttgaa tcttctcctg
     5881 gcgcttgcgc ctgatcctcg ttagcctccg caactcaaaa tgcccccggt aatcgggagc
     5941 tcaataagta cgaggcctac tacgaatcga ccggacaccg catacctccg gaggccatgc
     6001 ctccatccga ccgcagcata cgactgcaca ccttttcatg tcaaacggcg caatggggaa
     6061 atggtcccct aacgggaccg ctgctcaggc gatatcaggc gtcgaagggc ttggctcagg
     6121 actcgccaca gcagcttcga cgacctggac agtgccgacc attgccggat gcaagccgca
     6181 ttggtattca aacgtgccgg ccctatcgaa ggtgtgctcg aaggtgccgc ccggattcag
     6241 aatgtccgac gaggcccaat cagcaagaat tgtgtgcggt gtctgccc
//
LOCUS       DATAOZ010000561         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2850190, whole genome shotgun sequence.
ACCESSION   DATAOZ010000561 DATAOZ010000000
VERSION     DATAOZ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2850190"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..899
                     /locus_tag="VHR64_14170"
     CDS             423..899
                     /locus_tag="VHR64_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304029.1"
                     /translation="MRKLFRSLIGALVALTFLSGLVAPHADAARGWCKADPVIMVDGQ
                     LADVFVGSSLDMLLKATGPIKMEIIIPSGSTGGVVLTDAGFLRGYSITFKHSSTLTKT
                     KSHTQVKIRVYAPATTSTLPVTVTFAPRSLGSSLTDILFGTSVTGKSNQWVSLTTR"
     gene            1010..2293
                     /locus_tag="VHR64_14175"
     CDS             1010..2293
                     /locus_tag="VHR64_14175"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX3304030.1"
                     /translation="MAISVGGPACAIAWYFLAAADTSIGFLTLVLVGALAALAERFQV
                     HLTHHTYVSTSTVIYIAMLLTLPLPLCGVIALLASAAAQAHRLWSRPALGIDEPLFNT
                     GQTALYVTAAAACVELAPSWPASVAGIPIAHVLVASAALHLGNGLLVAGASARHLGRS
                     TSRVWRHNLLLDLGPHAAMTLMGVCAAELLSGSPLLLPTLAVPAVLIHRAVQQSTELR
                     ENVREALASLVDVVELRDPYTAGHSRRVAELARAIAIQLGLTAEEADAIEDAGNVHDL
                     GKVAIDPAILLKPGKLTEAEWAEMKRHPVYGGEVLARFASYRSGIPLVRSHHESWDGS
                     GYPDGLRGTDIPLGARILAAADTFDALTSDRPYRAGMDLDRARAILQDGKGSHWDPAI
                     IEALFRVLDARGPEVDEVSTGTGASDSAPADARAA"
BASE COUNT          413 a          783 c          769 g          481 t
ORIGIN      
        1 cgaatatgac gacaatgcga acgaaccgtg acatggagtg aactcctcca gcgggatggc
       61 ggctcacagg tgcgcgaaga cctaccgctt cggcgtcgca gaccggcaat ctcaccggag
      121 catgacccat tcgggctagc ccgatcggcg atatcccgat agtaagtgga tgattgacgt
      181 aggtcaatca ctttcccaac tgtgacttac gacggatcca ggcagcgccg actgatgggc
      241 ccggcaattc gccgggcctg tgcattcgct tcagttcgcg accttggtac cgggccgatc
      301 ccccgaacgt gccaaccggg ttgcccaccc accggtcatg gcgcgattta cctagtccac
      361 cgatgctgat gggagtcggt gggccgaagg tgctcgtcgt tggagcgtcc gggaggtgtg
      421 acatgcgcaa attgtttcgg tcgttgatcg gggcgctcgt cgcgctcacc ttcctgagcg
      481 gtctggtagc tccgcatgcg gatgcggccc gcgggtggtg caaggctgac ccggtcatca
      541 tggtcgacgg ccagcttgcg gatgtcttcg tgggcagctc gctggatatg ctgctgaagg
      601 cgaccggtcc gatcaagatg gagatcatca ttccatccgg ctccacgggt ggcgtggtcc
      661 tgaccgatgc cggattcctc cgcgggtaca gcatcacgtt caagcattcg agcacgttga
      721 caaaaacgaa atcccacact caggtgaaga tccgggtgta cgcgccggcc acgacctcca
      781 cattgccggt caccgtgacc ttcgcgcccc gcagtctcgg cagttccctg accgacatcc
      841 tcttcggcac aagcgtcacc gggaaatcga accaatgggt ttctctgacc acgcggtagg
      901 gtgatcgatt ggctagtcgt cttcctccgc cgctcactcg gccgtgatga gccacccgag
      961 accgtggaat agctatcgcg gtcttccgcc gtcactgatt cgctacctcg tggcgatcag
     1021 cgtcggcgga cctgcttgtg cgatcgcgtg gtacttcctg gcggcggctg atacctcgat
     1081 cgggttcctg acgctggttc ttgtcggagc cctggcggcc ctggctgaac ggtttcaggt
     1141 tcacctgacg caccatacct acgtcagcac cagtacggtc atctacattg cgatgctcct
     1201 gaccttgccg ctcccgctct gtggcgtgat cgcgttgctg gcatcggcag ccgctcaggc
     1261 gcatcgactc tggtcccgac ccgctctggg cattgacgag ccactcttca acaccggcca
     1321 gacggccctc tatgtgaccg cggcggcggc gtgtgtcgag ctggctccgt cgtggccggc
     1381 cagcgtcgcc gggataccaa ttgcacatgt cctggttgca tcggcggcct tgcaccttgg
     1441 caacggactg cttgtcgccg gggcatccgc gcgccatctc ggccgttcaa catctcgcgt
     1501 ctggcgtcac aacctgttgc tggatctcgg accgcatgcg gcaatgaccc tgatgggcgt
     1561 ctgtgccgcg gagctgctga gtggctcgcc gctgcttctg cccacgctgg ccgtgccggc
     1621 agtgctcatc catcgcgccg ttcagcaatc tacggagctg cgggaaaacg tgcgggaagc
     1681 gcttgcgtcg ctggtcgacg tcgtagaatt gcgcgatccc tacacggcag gtcattcccg
     1741 ccgggttgcg gagcttgcgc gcgcgatcgc gatccagctc ggattgaccg cggaggaggc
     1801 cgatgcgatc gaggacgccg gcaatgtcca tgacctcggc aaggtcgcga tcgatccggc
     1861 gatcctgctc aagcccggga agctcaccga ggccgaatgg gccgagatga aacggcaccc
     1921 ggtctacggc ggggaggtac tggccaggtt cgcatcgtat cgatcgggca tcccgctcgt
     1981 gcggagccac catgagtcat gggacggatc tggataccct gacggtctac gcgggaccga
     2041 cattccgctg ggtgcccgca tcctagccgc cgccgatacg ttcgatgccc tgaccagcga
     2101 ccggccctat cgcgccggca tggacctcga ccgagccaga gcgatcctgc aggacggcaa
     2161 aggctcgcac tgggacccgg cgattattga ggcgctgttc cgtgtcctcg acgcgcgtgg
     2221 tccggaggtc gatgaggtga gtaccggcac aggagcgtcc gattccgccc ctgctgacgc
     2281 acgcgcagcc tgaagcggaa atcaaatgcc gtgtccgcaa agtgcacgtg gcgcggggtt
     2341 accacgcccc gcttgatccg tccccaaata cgggtgatgg cgcccattca gtatcgggat
     2401 tggttcccct tcgaatgagc cgtcatcgag tccagttggg gcgtgg
//
LOCUS       DATAOZ010000562         4507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2061386, whole genome shotgun sequence.
ACCESSION   DATAOZ010000562 DATAOZ010000000
VERSION     DATAOZ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061386"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VHR64_14180"
     CDS             <1..1333
                     /locus_tag="VHR64_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX3304031.1"
                     /translation="DPRRIVSVAVTSFGEAGVLVDERGRPTTDMIAWFDRRTLDQCRR
                     FGERIDPDRLFSTCGTVVQQIMTAPKLMWHRENEPDAWARSTMFLMTADYVAYRLCGE
                     RAQNLSLSSRTALIDLRTRDWSDELLDLAGVDRSFLPPIVDGGTPLGCVTPEASTETG
                     LPTSTLVAVGGHDHVCGALAAGVVRHGDFLDSIGTTECLFVALDQPLANAAMGRQGYT
                     QGAHARERSYVYGGLYTAGICYDWIRQVGAGSTPHDELLASAEAVAPGSLGVTFIPHL
                     RLSNTPNMDSQARGAFIGLTTDAGTPELVRAVLEGVAFEGRASIEPLLRFAGLPPLTD
                     AMVVGGTARNELLLRIKASVMNMRMHVLEIEEAGALGAAMLGGIAAGVYHSVDDAVQT
                     VQRADHVIEPDPEAAVVYDTIFTQVYQHMYDALRPFNHRLYELFTGAELGN"
     gene            1330..2895
                     /locus_tag="VHR64_14185"
     CDS             1330..2895
                     /locus_tag="VHR64_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX3304032.1"
                     /translation="MTNSGADRTGPLLVGLDVGTTNIKAVVVRPNGEPVAIASAQQEI
                     DYPQPGWACHKPERIWEICCQVLRDAVAQVPDPGQIQGVAVASMGEAGVTLDEHGEAT
                     YDVIAWFDRRTVQQTDQFSAQIDSDELFAITGTELQPIMTAPKLMWIRDALPEVWART
                     RTFLNVSAYVAHRLGADYAQDHSLASRTGLFDLRCRDWSDRLMEIADLNPGIFGDLAP
                     GGTRIGSVSTDAATATGLPAGIAIGLAGHDHICGAFAAGVIRRGDVLDSIGTAEGLLV
                     CLDQPVEDVTMGGQGFTQGAHVVPDQYYGLGAIYTAGASYEWARQAVGGGRSHDDLLA
                     GARSVPAGSLGVLFLPHLRLAMFPELDSRARGAFVGLTTDVTSDAMIRAVLEGVAMEG
                     RAGFGPLARYARLPKLTDIKVIGGTSRNQLLIEIKASVMNARHHVLEIPEATALGAAM
                     LAGMAAGVYRSADDAIAAVRSDARVVEPDIDVVALYDTLFEDIYRHLYGALRPMHHRL
                     FDLFLSSHEVGAA"
     gene            2892..4397
                     /gene="xylB"
                     /locus_tag="VHR64_14190"
     CDS             2892..4397
                     /gene="xylB"
                     /locus_tag="VHR64_14190"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEX3304033.1"
                     /translation="MSYLGIDLGTTSLKAVLVSVSGEVLREGTASYSMQHPYPGAAVQ
                     DPEHWWRATRDAVQQIQNGLGAEINAIGLSGQMHGTVCLNSMRQLIRPALIWPDTRGT
                     ITAAELTAVVGEMRLSDTIGTPLASGFQGVTVAWLRQHEPTTWRSIATVLLPKDYLRY
                     RMTGEIATEPSDAAGTGMLDVHTRDWSDLMLDTVGLDRGKVPAIRPSAQIAGRLTSEA
                     AEYLGLPVGLPVVTGGGDAPLAALASGVADGRSLLATLSSGAQVIAFTDHPSVDPRLR
                     VHTFAAPLDPTKGEAGWYVMGATMVAGMALSWLKENIFESSSDQAITSMVQRASEISP
                     GSNGLLFAPYLTGERTPHLDSRARAAFLGLTADHDRRHLTRAAMEGVVFALRDALDVV
                     RSLTRTGHDVVLAGGGSRSPLWRQIMADIFEMPVRPSLIVDQSAIGAALLAAACDLGT
                     TASALGKEWSRFDRSVEPVPANMIRYEELRGIFRDIYPAHAAHFHRLSEFD"
BASE COUNT          723 a         1455 c         1523 g          806 t
ORIGIN      
        1 ggacccgcgc cggattgtct ctgtcgccgt caccagcttc ggcgaggcgg gcgtgttagt
       61 tgacgaacgc ggccggccga ccacagacat gatcgcctgg ttcgatcggc gcacgctcga
      121 tcaatgccgg cgctttggcg agcggatcga tccggatcgc ctcttctcga cctgcggcac
      181 ggttgtccag cagatcatga ccgcgcccaa gctgatgtgg caccgtgaga acgagcccga
      241 cgcctgggca cgttcgacga tgttcctgat gaccgccgac tacgtcgcgt accggttgtg
      301 cggcgagcgc gcacagaacc tctcgctttc ctcccgaacc gcgctgatcg acctgcgcac
      361 gcgcgattgg agcgacgagc tgctggatct ggccggcgtg gaccgttcct tcctgccacc
      421 gatcgtcgat ggtggcacgc cgctcgggtg cgtgacccca gaggcgagca ccgagacagg
      481 cctgccgacc tctacccttg tcgcggtcgg ggggcacgat catgtctgcg gtgcgctggc
      541 ggccggggtt gtccggcacg gtgatttcct ggactcgatc ggcacgaccg agtgcctctt
      601 cgtcgcgctc gatcagccgc tcgccaacgc ggcaatgggc cggcagggct acacgcaggg
      661 cgcgcatgcc cgggagcgct cgtacgtcta cggcggcctc tacacggccg gcatctgcta
      721 cgactggatc cggcaagtcg gcgccgggag cacaccgcac gacgagctgc tggcgagcgc
      781 ggaggcggtc gcgccgggca gcctcggcgt caccttcatc ccgcaccttc ggctcagcaa
      841 cacgccgaat atggactcgc aggcgcgcgg cgcattcatc ggcctgacga ccgatgccgg
      901 aacgccggag ctggtccgcg ccgtcctgga aggcgtcgcg ttcgagggca gggcatccat
      961 cgagccactg ctcaggttcg ccgggttacc accgttgacg gatgcaatgg tcgtcggcgg
     1021 gacggcgagg aacgagcttc tgctgcggat caaggcgtcg gtcatgaaca tgcgcatgca
     1081 cgtgctcgag atcgaagagg cgggtgcact cggcgcggcc atgctcggcg gcattgcggc
     1141 cggcgtctat cactcagtcg atgacgcggt gcagacggtc cagcgtgctg accacgtcat
     1201 cgagccggac cccgaagcgg cagtcgtata cgacacgatc ttcactcagg tttaccaaca
     1261 catgtacgat gcgctccgac cattcaacca tcgcttgtac gaactcttca caggtgcgga
     1321 gctgggcaat tgaccaattc gggagccgat cgcactgggc cgctgctcgt cggcctggat
     1381 gtcggcacaa ccaacatcaa ggcggtcgtc gtccgtccca acggggaacc ggtcgcgatt
     1441 gcttccgccc agcaggagat cgattacccc cagccggggt gggcatgcca caagccggag
     1501 cggatctggg agatctgctg ccaggtgctc cgcgacgcgg tcgcgcaggt gccggatcct
     1561 ggtcagattc agggcgtggc cgtggctagc atgggcgagg cgggtgtcac cctcgacgag
     1621 cacggcgaag ccacgtacga cgtgatcgcc tggttcgacc gccgcacggt ccaacaaact
     1681 gaccagttct cggcgcagat cgattcggac gaactcttcg cgatcaccgg gaccgagctt
     1741 cagccgatca tgacggcgcc gaagctgatg tggattcggg acgcgcttcc ggaggtctgg
     1801 gcccggaccc gcacgttcct gaatgtgtcg gcctacgtcg cgcaccggct gggcgccgac
     1861 tatgcgcaag atcactcgtt ggcatcgcga acgggtctgt tcgatctgcg atgccgggac
     1921 tggagcgacc ggctgatgga gatcgccgac ctgaatcccg ggatcttcgg agatctggcg
     1981 ccaggaggca cccggattgg ctcggtctcg acggatgccg cgaccgcgac cggtctcccg
     2041 gccggaatcg caatcggcct cgcggggcat gaccacatct gcggcgcctt cgcggccggc
     2101 gtcatccggc gcggtgacgt gctcgattcg atcgggaccg ccgaggggtt gctggtctgt
     2161 ctcgatcagc cggtcgagga cgtgaccatg ggcggccagg gattcacgca gggcgcgcac
     2221 gtcgtgcccg accagtacta cggcctgggc gcgatctaca ccgccggggc gagctacgag
     2281 tgggcgcgac aggcagtagg gggtgggcgc agtcacgacg acctgttggc gggagcgcga
     2341 tcagttccgg ccggttcgct cggcgtcttg ttcctgccgc atctgcgcct ggcaatgttc
     2401 ccggagctcg attcgcgcgc gcggggcgca tttgtcggcc tgaccaccga cgtaacgtcg
     2461 gacgcgatga ttcgcgcggt gctcgaaggc gtggctatgg agggaagggc ggggttcggc
     2521 ccactcgccc gctatgcccg gctgccgaag ctgaccgaca tcaaggtgat cggcggtacg
     2581 tcgcgcaatc agctgctgat cgagatcaag gcgtcggtca tgaacgcgcg tcaccatgtg
     2641 ctcgagatcc ccgaagcgac agcgctcggc gcggcgatgt tggccgggat ggcggcgggc
     2701 gtttatcgtt cggctgacga cgccatcgct gccgtgaggt ccgatgcccg ggtcgttgag
     2761 ccggacatcg atgttgtcgc gctctacgat acgttgttcg aggatatcta ccgccacctg
     2821 tatggggcgc ttcggccaat gcatcatcgt ctcttcgatc tattcctgtc atcacacgag
     2881 gttggggccg catgagttat ttgggaatcg atcttggcac gacctcactc aaggccgtgc
     2941 ttgtcagcgt ctccggcgaa gtattgcggg aggggacagc cagctattcc atgcagcacc
     3001 cgtatcccgg ggctgcggtc caggacccgg agcactggtg gcgggcgaca cgtgacgcag
     3061 ttcagcagat ccagaacgga cttggcgccg agatcaacgc gatcggcctt tccggccaga
     3121 tgcacgggac ggtctgcctc aactcgatgc ggcagctcat ccgacccgcc ctgatatggc
     3181 cagatacccg agggacgatc acagccgcgg aactgaccgc ggtggtcggc gagatgcgcc
     3241 tgtcggatac gatcggtacg ccgctcgcgt ccgggtttca gggtgtgacg gtcgcgtggc
     3301 tgcggcagca cgagccgaca acgtggcggt cgatcgcaac cgtactcctc ccgaaggatt
     3361 acctgcgata ccgcatgacc ggcgagatcg cgactgaacc gagcgatgcc gcgggaaccg
     3421 gcatgctcga cgtccatacg cgggactggt cggatctgat gctcgacacg gtcggcctgg
     3481 atcgcggcaa ggtgccggcg attcgaccgt cggctcagat agccgggcgg ttgacgtccg
     3541 aggcggccga atacctgggg ttgccggtcg ggttgccggt cgtcactggt ggcggcgatg
     3601 cgccgctggc cgcgctcgcg tccggtgtcg ccgacggccg ctcgctgctg gcgacgctca
     3661 gctcaggcgc gcaggtcatc gcctttaccg atcatccttc cgtcgatccc cggttgcgag
     3721 tccatacctt tgcagcgccg ctcgatccga ccaagggtga ggccggctgg tatgtcatgg
     3781 gcgcgaccat ggtcgccggc atggcgctga gctggctcaa ggagaacatc ttcgagtcgt
     3841 cgtcggatca ggcgatcacg tcgatggtgc agcgagcctc ggaaatctcg ccggggtcga
     3901 acggtctcct cttcgctccc tacttgaccg gcgagcggac gccccacctc gactcgcgcg
     3961 cccgggccgc gttcctgggc ctgaccgccg atcacgaccg gcgccatttg acccgcgcgg
     4021 ccatggaagg cgtcgtcttc gcgctgcggg acgcgcttga tgtcgttcga tccctgactc
     4081 gcacaggtca cgatgtcgtg ctggccggcg gtggctcgcg cagtccgctc tggcgacaga
     4141 tcatggccga tatcttcgag atgcccgtcc ggccgtcgct gatcgtggat cagtcggcca
     4201 tcggcgcggc gctgcttgcg gccgcgtgcg atctgggcac gacggccagc gcgcttggca
     4261 aggagtggtc gcgattcgat cgttcggttg agccggtgcc ggccaacatg atccgctacg
     4321 aagagctgcg tggcatcttc cgggacatct acccggcaca cgcggcgcac ttccaccgcc
     4381 tgtccgaatt cgactgaggc cgcaagtcaa ttggcaaaac gtggcatggg gtaaccacgc
     4441 cgcgtccggt cttgcacgtc gtttcttcgg atgggaaccg tccccaattc ccgaccctgt
     4501 tccgtga
//
LOCUS       DATAOZ010000563         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1533368, whole genome shotgun sequence.
ACCESSION   DATAOZ010000563 DATAOZ010000000
VERSION     DATAOZ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533368"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VHR64_14195"
     CDS             <1..1061
                     /locus_tag="VHR64_14195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX3304034.1"
                     /translation="AGYRLQAIAAATRPLSAFAHDGARGARIGPAPYPPIFLDFFSFF
                     TAPSPVVGYLLWTAVSAIVALLVFRRLAARAHLQSPWLYALFLVSSFPVMMALFVGQV
                     VSILLVCVLLACLDFEDGRELRAGFWVGLLALKPQYAPCLVLVILLKRRWAALAGVSL
                     GGGTVLVASLITGGLSGVVAYGRELATSYPSYAGSLGVDPRGQLSWRSVVAMTLPGLG
                     SGWSLALVIVLSLLTILTLPLIWRGPWSPHSDDFALRFLATLAVTLLVAYQSQPHGAA
                     LLLIPGVLVASKSRSRAFLPLLFCLAIAAAPWIGLVSALVLGNLWLVSLAITGVLVLV
                     VILTLGRVRWTVDPAFVA"
     gene            1138..2130
                     /locus_tag="VHR64_14200"
     CDS             1138..2130
                     /locus_tag="VHR64_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3304035.1"
                     /translation="MPNTEISLTPSGIHPWVAEGQQRVRFGISIFPQPADWHQFIEIV
                     QRAEADGIDGYFSYDHPQSRADCWTALSALAACTTTIRLGTAVDCIYYRSPYMLARQA
                     ADVDRLSGGRLVLGIGIGDNVPEFHRMGLEFPPTKVRQQGLVETITILRGLWSGKPFR
                     HEGEQWSAFSDTAYPFFGPVQQPWVPIMIAGGGEKVTLRQVAQFADASNMGAHEWIGS
                     AVTSADIDRKFGKLRDYLTEFGRPFESILRSQFTMPLVMAETQAELDRKLAGMPQDVL
                     EWCGDALFAGTPEETIDFYQDMVAAGFQYFIANILDRDYSTIELMGKYLIPAFA"
BASE COUNT          310 a          722 c          730 g          479 t
ORIGIN      
        1 cggccggcta tcggctgcag gcgatcgccg ccgcgacgcg accactttca gcgttcgccc
       61 acgacggtgc acggggcgcg cgcattgggc cggccccgta tccgccgatc tttctcgact
      121 tcttctcctt cttcaccgcc ccttcgccgg tcgtcggata tctcttgtgg actgccgtgt
      181 cggcgatcgt cgccttgctc gtgttcaggc gactggcggc gcgcgctcac ctgcagtcgc
      241 cgtggctgta tgccctcttc ctggtgagct cgtttccggt gatgatggcg ctcttcgtgg
      301 gccaggtcgt ctcgatattg ctcgtctgtg tcttgctcgc atgcctcgat ttcgaagacg
      361 gacgagagtt gcgagccggc ttttgggtgg gtttgctcgc gctcaagcca cagtacgcgc
      421 cgtgtctcgt actggtcatc ctcctgaagc ggcgctgggc cgcgctcgcc ggggtcagcc
      481 ttggcggcgg tacggtactg gtggcgtcgt tgatcaccgg cggcttgagt ggcgtcgtcg
      541 cgtacggccg cgagcttgcg acgtcatatc cctcctatgc gggcagcctt ggcgtcgatc
      601 cgcgcggtca actgagctgg cgttcggtcg tcgcgatgac gctgcccggc cttggctccg
      661 gatggagcct ggccctggtc atcgtattgt ccctcctgac cattctgaca ttgccgttga
      721 tttggcgcgg tccgtggagt ccgcactccg acgactttgc ccttcgattc ctggcgacgc
      781 tggcggtcac gctgctcgtg gcgtaccaga gccagccaca cggagcggcc ctcctcctga
      841 ttccgggggt tctggtcgcg agcaaatcgc ggtcgcgcgc gtttcttccg ctccttttct
      901 gtttggctat cgcggcggcg ccgtggatcg gcctcgtgtc cgccctggtt ttgggcaacc
      961 tctggctcgt ctccctggcc ataacaggag tgctcgtttt ggtcgtcatc ctgacgctcg
     1021 gtagagtccg ctggacagtc gaccctgcat ttgtggcttg agcgggcggc ggtgtgcgct
     1081 tgcgtagaat gtcgcgagaa cgtcgcgatc tgacccggtc atgtaaagtg agcgccgatg
     1141 ccgaacaccg agatttcgtt gacgccctcc gggattcacc cgtgggtggc ggaggggcag
     1201 cagcgggtcc ggttcgggat cagcatcttt ccgcagccgg ccgactggca tcagttcatc
     1261 gagatcgtcc agcgcgccga ggccgacggc attgacggct acttcagcta cgaccatccg
     1321 cagtcccgcg ccgattgctg gaccgcgctc tctgccctcg ccgcatgcac cacgacgatt
     1381 cggctcggca ccgcggtcga ctgcatctac taccgctcgc cctacatgct ggcccggcag
     1441 gcggccgatg tcgatcggct tagcggcggt cggctcgtcc ttggtatcgg gatcggcgac
     1501 aacgtccccg agttccaccg gatgggcctc gagttcccgc cgacgaaggt ccgccagcag
     1561 ggcctggtcg agacgatcac gatcctgcgc gggctctggt cgggcaagcc gtttcgacac
     1621 gaaggagagc agtggtcggc gttcagcgat accgcttacc cgttcttcgg tccggtgcag
     1681 cagccttggg tgccgatcat gattgccggc ggcggcgaga aggtgactct ccggcaggtc
     1741 gcgcagttcg cggatgcctc gaacatgggc gcgcacgagt ggatcggcag cgccgtcacc
     1801 agcgccgata tcgaccgcaa gttcggcaag ctgcgggatt atttgactga atttggacgt
     1861 ccgtttgagt cgatcctgcg cagccagttc acgatgccgc tcgtgatggc cgagacccag
     1921 gcggagctcg accggaagct ggccgggatg ccgcaggacg tgctcgagtg gtgcggcgat
     1981 gcgctctttg ccggcacgcc cgaagagacg atcgacttct accaggacat ggttgccgcc
     2041 ggcttccagt acttcatcgc caatatcctc gaccgcgact attcgactat cgagctgatg
     2101 ggcaagtacc tgatcccggc cttcgcgtag ccgcacgacc gtcagacagg gaatgaggga
     2161 ccgggcggtc cctcattccc tgtggatgtg tgtccggcgc cgcccgattg aatggctggc
     2221 gggatcaacg acggcccgga c
//
LOCUS       DATAOZ010000564        13784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2328711, whole genome shotgun sequence.
ACCESSION   DATAOZ010000564 DATAOZ010000000
VERSION     DATAOZ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13784
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328711"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..642
                     /locus_tag="VHR64_14205"
     CDS             229..642
                     /locus_tag="VHR64_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304036.1"
                     /translation="MIIAGGVIAGIGTFFRDIITGQNDQWTFILDTIGLLIAVLGAVL
                     DFRATGRVAEFAPGFGRRRAALELVIGAILALSGCVLLGWVVTEAAPALVHGVLSAAL
                     TAGFGLVFASLLYFGWFSGTEYLGRQIEQRSDEEW"
     gene            1189..3249
                     /locus_tag="VHR64_14210"
     CDS             1189..3249
                     /locus_tag="VHR64_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing C40 family peptidase"
                     /protein_id="HEX3304037.1"
                     /translation="MRLREEPSYSGTLIRFLPEGTVLDVVDGPITDGDGNIWYQLSFE
                     GDTGYIVSDYLALDNGAAPRTTNAAPGAVVGSALIAGTNNDGVRCRTTPSTGGAIITV
                     VPEGAIVDLIGAAESGWQPINCAGSAGYVSTEFVSYDFSGGADFGAAEATGQATVAGT
                     NGDGVNCRTSATTSAAVITILSEGSVISLRGDAQAGWQPVVCAGSNGYVSTDFVSYDG
                     GTGGGADLAVGSEATGSLVVSGTNGDGVRCRTRASLDGSIITVLAEGTTVSTRGSEKN
                     GWTPIICAGTNGWASSQYLGSGSGNGGGGGTGDTAVVTGTGGGLRCRADAGYNASVLA
                     VLSDGTSVELRGGAQGDWQPVVCQGMKGYVFSGYLDYSGNGGGGNSGGGDSNSSLTSG
                     DSAHVSGTNGDGVRLRSSASFNGAIVMVVSEGQTVSVIDGSTGDWVAVSYRGTSGFIH
                     MDYLAAGDGSGNDGGDSGDTSGGLSVGDHARTTSDLNLRYDPSMGGGVAAVAPRGTVV
                     EITGGMSNGFYAVDWDGLSGYMHGDYLSWTDKALTKRGGSGEDPGDGGGGSNPTPTGN
                     AIVDYAMQYLGYPYVWATHGPSSFDCSGFTYWVVLNTLGINISPGTSAQINYGTPVSR
                     SSLQPGDLVFFQNTYTWGLSHVGIYIGNGKFIHAENESTGVVISDLSSQYYSSRWYGA
                     RRVA"
     gene            3707..3988
                     /locus_tag="VHR64_14215"
     CDS             3707..3988
                     /locus_tag="VHR64_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304038.1"
                     /translation="MVNVTQPRWQDVSASAGREISLPTSGAVEIQFWSDNATVDPAVC
                     RVIILEPQAALAQMASSTHAGAPVAYTDARFRPGFADEEEPTWEDAAWQ"
     gene            complement(4019..4273)
                     /locus_tag="VHR64_14220"
     CDS             complement(4019..4273)
                     /locus_tag="VHR64_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304039.1"
                     /translation="MDDHNEITLQTFAMLIVSELATFGLASKVAFTNEATPTVYVWRV
                     GDPGPEDVHEIDVRELSRNEVRRVVAAALQRFPGGNGHPL"
     gene            complement(4492..5610)
                     /locus_tag="VHR64_14225"
     CDS             complement(4492..5610)
                     /locus_tag="VHR64_14225"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX3304040.1"
                     /translation="MTEVSLHAVRGFRMRRQHLIERAPAGNLLEGVEAVHGIQAQIAA
                     HAQFAVAQRIETCRPPEIDRALWEEKSLIKTWAMRGTVHWLPATEEPLFVAGMRVVRL
                     PSIDRWWRNEGITPGQVKQFHAAVLEALANGPMTRQEVAAAVVPEIGDWAEPYLLSSW
                     GSGFKRMCQAGLIVFGPSRGTNVTFARRDSWTPHDEWADGDAALRELIRRYVRSFGPV
                     TVQDAAYWLGATIRDIQGAWDALLPELASVRVDGRERWLLAGDVDDLTAMEGAQLPVR
                     LVPAFDPLLLAHREKTDLLPETLRRRIYAAAAWVYPAILVGGAIAGKWQYERKPRQMI
                     VTVEPFQKLTKTTRRSIEREANYLAKLTGRTANVRYAE"
     gene            complement(5607..6557)
                     /locus_tag="VHR64_14230"
     CDS             complement(5607..6557)
                     /locus_tag="VHR64_14230"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX3304041.1"
                     /translation="MAVSASPARPIASSTASAAEHPLFAQAAPLVFVFIWSTGFIGAR
                     YGLPYAEPFTYLALRMIIAGGLMAAFATVRRSGWPDSWRQTGHIAVSGLLLHAGYLGG
                     VFFAISRGMPAGISALVVGLQPILTAVIASILLREHVSPRQWIGLALGFAGVAIVVIE
                     RMRINEGGDRSSALAADVAILIALLSTTAGTIYQKRHNTRMPLASGTAIQYGTTALAV
                     GLLAVLTETMHIDWNIRFIFALSWQVVVLSLVAITLLMILIRQNSVSRLSSYLYLVPP
                     LTAIEAYLLFNERLSLAAIGGMALVAVGVALVVVRRTAQA"
     gene            complement(6597..7355)
                     /locus_tag="VHR64_14235"
     CDS             complement(6597..7355)
                     /locus_tag="VHR64_14235"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304042.1"
                     /translation="MLRELTREDWLSILNLDERHVPETLMLRGTRNLRRNFEKYRRLF
                     ADALEIGSPNGLFEDILVGKIGERQVGYASVYGPAMASEITHVFGVLGTRRVVQTGVC
                     GALGVEMVAGDLVIGREAVCGEGAVQCYLPDAECIEATPTLVERATALAGPGGTHIGR
                     IWTTAALLAEGLEDLTRWQQTGAVAVDMETATTYVVAEWTRMERVAVLSVFDNPLAGA
                     HIALEEAEKQTLRERGEQRMLEVVLGLVAGDEPK"
     gene            7422..8144
                     /locus_tag="VHR64_14240"
     CDS             7422..8144
                     /locus_tag="VHR64_14240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3304043.1"
                     /translation="MTEAKRPIADWLTRVRTVWNDRAPDWDKMSEANALSEDRTADIA
                     RLTVGLDLHHGSTLLDAGCGTGQWSIAFGTLGCAVTAQDLAPAMVDRARDHAKDRGVS
                     IDFRTGDISALPEPAATFDAIFGRCVLLFVPDPPAVLREFRRVLKPGGRIFVSVPGAL
                     SPIYNRSWRRFVEPGNVGTNFMVPWELETLLAEGGWRVLEQWGDFSQNLMGAANPFEA
                     AVRNAPRELQQAAATTWGFIAE"
     gene            8141..8872
                     /locus_tag="VHR64_14245"
     CDS             8141..8872
                     /locus_tag="VHR64_14245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3304044.1"
                     /translation="MRIDFNTYADAYQRNRAVQPKVLLALIEGGDLARGDAVLEIGCG
                     TANYLAAIVAETGARGTGVDPAERMLAEVRLDPEKDDLELLLGSAEALPVADSAARFA
                     YSVDVIHHVGDRAMAAREAFRVLAPGGRLCIATDSAQDIANRVPLASHFPETVAHELR
                     RYPAIGTIITELADAGFAELKTTHVCHEYALTDVVPYRERAFSSLRLITDDELAAGLT
                     RMERDLADGPIAARSVYTLVWATRP"
     gene            complement(8915..9637)
                     /locus_tag="VHR64_14250"
     CDS             complement(8915..9637)
                     /locus_tag="VHR64_14250"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX3304045.1"
                     /translation="MVGPRGRPGPGTNRIFALLPPDVNERVRRKCRPVHFELGQVILP
                     QDGRIEHAWFSTGVVFSAVVRMDDGRSVEAVPAGFEGMVGVATLLGESISQYEVTIQG
                     AGDALQISVPDLLRLAEDEPAVRDLLLRYAQIQMIQAARSAACNRLHDVEERLARWLL
                     HMHDWIWADHMQLTQEFLAIMLGVRRASVTVAARTLQNAGLISYARGEIMILDRDGLE
                     AVACEDYFTIRDAFDRLLPLEG"
     gene            9780..10166
                     /locus_tag="VHR64_14255"
     CDS             9780..10166
                     /locus_tag="VHR64_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304046.1"
                     /translation="MLTPDILAVISDRCVAARLRGDRTVSIDLFDPEIDAQPWSWHID
                     VSLMCDWIARHWRPEGPAEPSAYPDAVSFLGQQRDSAGPRWYAAQDMILGYIPGTVHG
                     CYAISVWSELPGLLSAAPEPGMESWA"
     gene            10163..10528
                     /locus_tag="VHR64_14260"
     CDS             10163..10528
                     /locus_tag="VHR64_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304047.1"
                     /translation="MTPRRIVRNDSVMGGRWHFEGTDIAITAVRADAIAGVGNLAHIC
                     QRTGLTEEEIMLALGFEFPEVRAPQLWSQYAAVTIACSCGEESHATFSGFDRRDILCA
                     CGRVWCLRLTLDADVHRAD"
     gene            10632..10991
                     /locus_tag="VHR64_14265"
     CDS             10632..10991
                     /locus_tag="VHR64_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304048.1"
                     /translation="MYAKLIIWRLGADHRDVAGYEQFLRDFAPKSLERLRQFGMLDGF
                     IIRLTHNTVLTVNLYETADGAYSAWHNVVSQPEYGAAGELELLFHLISRGDDLPMVGE
                     LALAADEKSTTKSTDNR"
     gene            complement(11023..11439)
                     /locus_tag="VHR64_14270"
     CDS             complement(11023..11439)
                     /locus_tag="VHR64_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304049.1"
                     /translation="MDESRFDAIVKSLATPRPRRAVFRALLGGIAAGAWARQATAAPV
                     RTCSGNNLECPGKQICGFDDATQQVICKDVTGYDHSEFRVCKGEFEFNLCQRGSQCCV
                     YPDIRDPGGISLVTNCCDNGLICDTQLGCVPRPGTA"
     gene            11837..12877
                     /locus_tag="VHR64_14275"
     CDS             11837..12877
                     /locus_tag="VHR64_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HEX3304050.1"
                     /translation="MKRVIIEQFGGPEVLNVVEVANPRPGPGEVSVRVLAAGVSFTDS
                     QLRAGTYLGGPKPPFTPGYELVGVVEELGPGCTRLRVGDRVGALTVWGADAERVCVPE
                     KYAVDVPEDLDPAEVVSLIFPYMTAYQMLHRTARVKCGESVLVHGAAGRVGTAALELG
                     AVAGLRQFGTCSARDRAQVEALGAVAIDYRSEDFLDRVRELTGGKGVDVVIDGIGGRV
                     SLRSFRALRVGDRIKGDPGGRLIMMGNYNTLQQGHKSWRGWLEWWPATAIVWTWGTIS
                     PRRRVMGYRVQKLRIDHQDWFAEDFRVLLELLRAGKIHPVVAERLPLIEARHAHELLE
                     STAAKGKLVLVP"
     gene            13244..>13784
                     /locus_tag="VHR64_14280"
     CDS             13244..>13784
                     /locus_tag="VHR64_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304051.1"
                     /translation="MTMTRQLPPTDDAAASLIGRRLTRRQTLALLGGGLTGGAMLGAV
                     TGDPGRSVDAAQITIEAGPSEAQSTGRPTIADIAAALQWDTERIFRFVADHVAYEPYV
                     GALRGAEGTLRAMAGNAVDQSLLLAALLAEAAVPYRFAIGQLSADGQTALEAQLAPTV
                     EELSDRKLRTFRNPTDPPDT"
BASE COUNT         2393 a         4414 c         4520 g         2457 t
ORIGIN      
        1 cctgcgccgc gaggtctggt cgctgcacga gcagcggttg cgggcgcggc tggagcaggt
       61 tcagcaggag ttggagcagg tcaccggcaa tcgccgcgtg atggagaccg aagcgcgagt
      121 aacgccggat caggaccagc caaagacaat ccgctccctc gacatgtgag tgccgtgctc
      181 cggcgatggt ggcgaaatcc tcaaactgcg ctccgcttaa cccttgtcgt gatcattgca
      241 ggcggggtca tagccggaat cgggaccttt ttccgggaca taatcacggg tcaaaacgac
      301 cagtggacct tcatcctgga cacgattggc ttgctgatcg ccgtcctcgg ggctgtgctt
      361 gactttcggg caacgggtcg ggtggccgag ttcgcgcccg gctttggccg ccgccgcgcg
      421 gccctggagc tggtgatcgg cgcgattctg gcgctttcag gctgtgtttt gctgggatgg
      481 gtggtgacgg aagcggcgcc ggctttggtg cacggagtgc tctcggcggc gttgactgcc
      541 ggatttgggc ttgtattcgc gagtctgctc tatttcggct ggttcagcgg aactgagtat
      601 ctgggacggc agatcgagca acggagcgac gaagagtggt gatcaccgcc gccatcgggc
      661 ttcgaattgc ataacttttg actgtgtcaa aggggaaaca ggttgacacc ctctccggag
      721 gatttgtatg gtgtccgtag atttagcgca aacggtgtac gtacgcggtg tatgagccgt
      781 tggcttgtct ggtttcggag ctttcgttca gctccgcgtt cgtcgaccag tagtctttcg
      841 tcatgcgaag cattgtgtga cggcggttgc tggtgcatgc tgcgctgctg attgaagttg
      901 gcgggcggcg ctaagtggtt gtggggggag tacccccagg tacggaggca agacaggttt
      961 cccgagttcc cggcacctgc gggaccaatt actcatggct gggtgattgg agtggaaatc
     1021 aggagggctg gaattttggc tggacaaccc cgacgcagcc gtggctggtt agcactaggt
     1081 attgtggcgc tcctggtcgc cacgttcttg aaccccctaa gcgcattcgc tgacactgac
     1141 ctcgtcatcg gaggtcaagc gcgcatctcg tacgccaatg gtgacgacgt gcgtctccgc
     1201 gaagagccca gctactcggg caccctgatc cgattcctcc ccgaaggcac cgtcctcgat
     1261 gtcgtcgatg gcccgatcac agacggcgac ggcaacatct ggtaccagct ttcgtttgag
     1321 ggcgacaccg gatacatcgt ctctgactac ctcgcgctcg acaacggcgc agctccccgg
     1381 accacaaatg cggcgcccgg cgcggtcgtc ggctccgcac tgattgcagg aacgaacaac
     1441 gacggcgttc ggtgtcgaac cactccgtcg accggcggcg cgatcatcac cgtcgtgccg
     1501 gaaggcgcga ttgtcgacct gatcggcgcg gccgagagtg gttggcagcc gatcaactgt
     1561 gctggctctg ccggttacgt cagcaccgag ttcgtttcct atgacttcag cggcggtgcc
     1621 gacttcggtg cggccgaggc caccggccag gcaacggttg ccggcaccaa tggcgacggc
     1681 gtcaattgcc gcacctccgc cacgacctcc gcggccgtga tcacgatcct ctccgaaggc
     1741 tcggtcatct cccttcgcgg tgacgcccag gcgggctggc agccggtcgt ttgcgccggt
     1801 tcgaatgggt acgtcagcac cgacttcgtc tcgtacgacg gcggcacggg cggcggagcg
     1861 gacctcgcgg tcggttccga ggcgaccggt tcgctggtcg tgagcggcac caatggcgat
     1921 ggcgtccgct gccggacccg cgccagcctc gatggcagca tcatcaccgt tcttgctgaa
     1981 ggcaccaccg tttcgacccg cggctccgag aagaatggct ggaccccgat catctgcgcc
     2041 ggcaccaatg gctgggcctc ctcccagtac ctcggctccg gtagcggcaa tggtggcggt
     2101 ggcggaaccg gtgataccgc ggtcgtgacc ggcacgggcg gcggacttcg ctgccgcgcg
     2161 gacgccggct acaacgcgtc ggtcctggcc gttctcagcg acggcacctc agtcgagctt
     2221 cgcggcggcg cccagggcga ttggcagccg gtcgtttgcc agggcatgaa gggctacgtc
     2281 ttcagcggct acctcgatta ctccggcaac ggtggtggcg gtaatagtgg cggcggagac
     2341 tccaactcgt cgctcacgtc gggcgattcg gcccacgtct ccggcactaa cggcgatggt
     2401 gttcgcctcc gctcatcggc cagcttcaac ggcgcgatcg tgatggtcgt ctccgagggc
     2461 cagactgtct ccgtcatcga cggcagcacc ggcgactggg tggccgtctc gtaccgcggc
     2521 actagcggct tcattcacat ggattacctc gcggctggcg acggaagcgg caacgacggc
     2581 ggcgatagcg gcgacaccag cggtggcctc tcggtcggcg accatgcccg gaccacgtcc
     2641 gacctgaacc tgcgctatga cccgagcatg ggcggcggcg tcgcggcggt cgctccgcgc
     2701 ggcacggtgg tcgagatcac cggcggcatg tcgaatggct tctacgcggt cgactgggac
     2761 ggcctctccg gctacatgca cggcgattac ctctcctgga ccgacaaggc gttgaccaag
     2821 cgtggcggct ctggcgagga tccgggtgat ggcggcggcg gaagcaatcc gaccccgacc
     2881 ggcaacgcga tcgtcgacta cgcgatgcag tacctcggct acccgtacgt ctgggcgact
     2941 cacggcccgt cgagcttcga ctgctccggc ttcacctact gggtggtcct gaacacgctt
     3001 ggcatcaaca tcagcccggg tacctcggcc cagatcaact acggcacgcc ggtcagccgc
     3061 agctcgctgc agccgggcga cctggtcttc ttccagaaca cgtacacgtg gggcttgtcg
     3121 catgtcggca tctacatcgg caacggcaag ttcatccacg ccgagaatga gtcgactggt
     3181 gtcgtgatca gcgacctgag ctcgcagtac tactcatcgc gctggtacgg agcgcgtcgg
     3241 gtcgcatagc cacgcacggc gggagccgag tgctccagcc aatgcgggaa tgggggaaca
     3301 cgacggggac tgcgatagcg gtccccgtca gtgtttgtgg cgttcagtgg gcagtgggca
     3361 gttcgggccc ggttggcgcg gcgcgggtcg ccccaagacc tctccgtccg atggtctccg
     3421 tcaccaggcg cgggttcgtg tccgccactt gactgatgcg cctgtgccgg ccggggccta
     3481 ccctcgtgac aaagcttcag accacatcga aggtcggaac ctgaacgaac ggagcatcca
     3541 atgttgccct ataccgcagt cgatctcatc cgctcgatga ccgtgaccac aacgaccccg
     3601 gagcgaaagg cagcgcgaag tgccgttttc acccggctcg cagggtttgt tgcgaggcac
     3661 ggcagcactg cgcgctagcg tggctcagta cgaaggagac aagtcgatgg taaacgtgac
     3721 gcaaccgcgg tggcaggacg tgtcggcctc agccggacga gagatatcgc tgccgaccag
     3781 cggcgccgtg gagatccagt tctggtccga taacgcgacc gtagatccag cggtctgccg
     3841 ggtcatcatc ctggagccgc aggccgcact cgcgcaaatg gcgtccagca cgcatgctgg
     3901 cgcgccggtc gcatacaccg atgcccggtt ccgtcccgga ttcgccgacg aagaggagcc
     3961 gacctgggaa gatgccgcat ggcaataagg agtcgtgggc gcagtgaggg atgggcggtc
     4021 agagcgggtg gccgttgccg cccgggaagc gctgtaacgc cgcagcgacg acccgcctga
     4081 cttcgttgcg gctcaattcc cggacatcaa tctcgtgcac gtcctccggg ccgggatcac
     4141 caactcgcca gacatagaca gtcggcgttg cctcattcgt aaacgcgact ttgctggcca
     4201 agccgaacgt cgccagctca ctgacgatca gcatcgcgaa cgtctgcagg gtgatctcat
     4261 tgtgatcatc catgagcggt tcccgggtca tgacgcggaa acattcggtg acggcagttt
     4321 accggagtct ggagtagacc acgacgggag tagcggagga atgagccgct gcggaaccga
     4381 gagagcagat tcccgcttca gtgaccgtcg tgggtgacgt ggtccaccgc aacgcatgat
     4441 tcgggacggc cgtcgggctg gattgggcgc aaagtattgc gcctctgcag gtcattccgc
     4501 gtagcggacg ttcgcggtac ggccggtgag tttggcgagg taattcgctt cccgctctat
     4561 cgagcgccgg gtcgtttttg tcagcttctg gaagggctcg accgtcacga tcatctgcct
     4621 gggttttcgc tcgtactgcc acttgccggc gatcgcacct ccgacgagga tagcggggta
     4681 tacccaggcc gctgcagcat agatgcgcct gcgcagggtc tcgggcagca ggtcggtctt
     4741 ctcgcggtgc gcgagtagca aaggatcgaa cgccggcaca aggcggacgg gcagttgcgc
     4801 gccctccata gcggtcaggt catcgacgtc cccggccagc agccatcgct cccgcccatc
     4861 aacccggacc gacgccagct caggcagcaa cgcatcccac gcaccctgga tatcgcggat
     4921 ggttgcgccc agccagtagg ctgcgtcctg cacggtgacc ggaccgaagg agcggacata
     4981 acggcgaatc agctcgcgca gagccgcatc tccatccgcc cactcgtcat gaggggtcca
     5041 cgaatcgcgc cgcgcaaaag tcacgttagt cccccggctc ggaccgaata cgatcagccc
     5101 ggcctggcac atccgcttga aaccggagcc ccagctcgag agcaaatacg gctcggccca
     5161 gtcgccgatc tccggcacga ccgccgccgc gacctcctgt cgggtcattg gaccgttcgc
     5221 aagcgcctcg agcacagcag catggaactg cttgacctgg ccgggtgtga tcccttcgtt
     5281 ccgccaccag cggtcaatgc tgggcaggcg aacgacccgc atcccggcca cgaagagcgg
     5341 ctcctcggtg gcgggtagcc agtgaacggt gccgcgcatc gcccaggtct tgatcagcga
     5401 tttttcctcc cagagcgcgc ggtcgatctc cggcggccgg cacgtctcga tccgctgggc
     5461 aacggcgaac tgggcatggg ccgcaatctg cgcctggata ccgtggacgg cttcgacgcc
     5521 ctcgagcagg ttgccggccg gcgcccgctc gatcagatgc tggcggcgca tccggaagcc
     5581 ccggaccgcg tgcaacgaaa cctcggtcat gcctgtgccg tccttctcac cactaccagt
     5641 gccacaccga ccgcgaccag cgccatgccg ccgatcgccg ccagactgag ccgctcattg
     5701 aacaggagat acgcctcgat cgcggtcagc ggcggcacca ggtacaggta actgctgagg
     5761 cgcgagaccg agttctgccg gatcaggatc atcagcagcg tgatcgcaac cagcgagagc
     5821 acgactacct gccacgagag cgcgaagatg aagcggatgt tccagtcgat gtgcatcgtc
     5881 tcggtcagga cggcgagcag gccgacggcg agggcagtcg tgccgtactg gatggccgtg
     5941 ccagaggcga gcggcatccg ggtattgtgc cgcttctgat agatcgtgcc ggcggtggtc
     6001 gaaagcaggg cgatcaggat cgccacgtcg gccgcaagcg cgctcgatcg atcacccccc
     6061 tcattgatcc gcattcgctc gatcacgacg atcgcgacgc cggcgaagcc gagcgccagg
     6121 ccgatccact ggcgcgggga gacgtgctcc cgcagcagga tcgaggcgat gaccgcggtc
     6181 aggatcggct gcagcccgac cacgagcgcg gagatcccgg ccggcatgcc ccggctgatc
     6241 gcaaagaaga ctccgccgag gtagccggca tggagcagta ggccagagac cgcgatgtgg
     6301 ccggtctgcc gccacgaatc gggccatccc gaccggcgga ctgtggcaaa ggcagccatc
     6361 aggccaccgg cgatgatcat gcgcagagcg aggtaggtaa agggctcggc gtatggcagc
     6421 ccataccgcg ccccaataaa cccggtgctc cagatgaaga cgaagacgag cggcgccgcc
     6481 tgcgcgaaaa gcggatgctc ggctgcggat gcggtgctgg atgcgatcgg ccgagccggg
     6541 gaagcgctaa ctgccatgcg ggtacctcgg cgtcattgcg aggggtgtgc attcgatcat
     6601 ttcggctcgt ccccagcgac caaccccagg acaacctcga gcatgcgctg ctcgccccgc
     6661 tcccggagtg tttgcttctc ggcctcctcg agggcaatgt gtgcgccggc gagggggttg
     6721 tcgaagaccg agaggaccgc gacccgctcc attctggtcc actcggcgac aacgtaggtt
     6781 gtggcagtct ccatatcgac cgcgaccgcg ccggtctgct gccagcgggt caggtcctcc
     6841 agtccctcag ccaggagcgc ggctgtggtc cagattcggc cgatgtgcgt gccgccggga
     6901 cctgccagcg cggtcgctcg ctcgaccagc gtcggtgtgg cctcaataca ttcggcgtcc
     6961 ggcaaatagc actgtaccgc tccctcaccg cagacggcct cacgcccgat gacgagatcg
     7021 ccggcaacca tctccacacc gagcgcgccg cagactcccg tttggactac gcggcgcgtc
     7081 ccgagcacgc cgaaaacgtg ggtgatctcc gaggccatcg ccgggccgta gaccgacgca
     7141 taaccaacct gccgttcgcc gattttgccg accaggatgt cctcgaacag gccattgggc
     7201 gagccgatct ccagcgcgtc ggcaaaaagg cggcgatact tctcgaaatt gcggcggagg
     7261 ttgcgagtgc cgcgcagcat aagcgtctcg ggcacatgac gctcgtccag gttcaggatc
     7321 gacagccaat cctccctggt cagctcgcgg agcacgatcg atcccctcgc aacgaatgcc
     7381 ggccacccgg cgcgactcgg aaggatagaa caggagagcg gatgactgag gcgaagcgac
     7441 caattgccga ttggctgacc cgcgtccgga ccgtctggaa cgaccgggcg cccgattggg
     7501 acaagatgtc cgaagccaac gctctttccg aagatcgcac ggctgatatc gcccggttga
     7561 ccgtcggact cgatcttcac cacggctcga ccctgctcga tgccggttgc gggaccggtc
     7621 agtggtcgat cgccttcggc acactcggct gtgcggtcac ggcgcaggat ctggctccgg
     7681 caatggtcga tcgcgcgagg gaccatgcga aggaccgggg cgtctcgatc gacttccgga
     7741 ccggcgatat ttccgcgctg cctgagccgg ccgcgacatt cgacgcgatc ttcggccgat
     7801 gtgtgctgct cttcgtgccc gatccgccgg cggtgctgcg cgagttccgg cgggtgctga
     7861 agccgggagg gcggatcttc gtgagcgtac cgggcgcgct ctcgccgatc tacaaccggt
     7921 cgtggcggcg cttcgtcgag ccgggcaacg tcggtacgaa tttcatggtg ccctgggagc
     7981 tggagacgct gctggccgag ggtggctggc gcgtgctcga gcagtggggt gacttcagcc
     8041 agaacctgat gggcgccgcc aatccattcg aggcggcggt gcgaaatgcg ccgcgcgagc
     8101 tccagcaggc ggccgcgaca acgtggggat tcatcgccga atgaggatcg acttcaacac
     8161 ctacgccgat gcctaccagc gcaaccgggc ggtccagccg aaagtgctgc tggcgctgat
     8221 cgagggtggc gacttggccc ggggcgatgc ggtgctcgag atcgggtgcg ggacagccaa
     8281 ctacctcgcc gcgatcgtgg cggagacggg cgcgcgtggc acaggggttg atccggccga
     8341 gcggatgcta gccgaagtga ggctggatcc ggaaaaggac gatcttgagt tgctactcgg
     8401 ctcggccgag gcgttgccgg tcgcggactc ggcggcccgt ttcgcgtatt cggtcgacgt
     8461 catccaccac gtcggggatc gggcaatggc ggcccgggag gcgttccggg tactggctcc
     8521 gggcggccgg ctctgcatcg cgaccgattc tgctcaagac atcgcgaacc gggttccgct
     8581 cgccagccac ttcccagaga cagtcgcaca cgagctgcgg cgctacccgg cgatcgggac
     8641 gatcatcacg gagctggccg atgcgggctt tgcggagctg aagaccacgc acgtctgcca
     8701 tgagtacgcc ctcaccgacg tcgtgcccta ccgcgagcgc gcgttctcgt ccctgcgctt
     8761 gatcacggac gacgagctcg cggcgggcct gacgcgcatg gagcgtgatc tggcggacgg
     8821 tccgattgcc gctcggtccg tctacacgct cgtctgggca acgcggccat agccggagtg
     8881 cctggccccg agaaggaacg gatttgctcc acgctcaccc ctcgagtggc agcaaccgat
     8941 caaacgcatc ccggatagtg aagtagtcct cgcaggctac cgcctcgagg ccatcccgat
     9001 ccaggatcat gatctcgccg cgggcgtagg agattagccc agcgttttga agcgtgcgcg
     9061 cggcgaccgt gacgcttgcc cggcgcacgc cgagcatgat cgccaggaac tcctgcgtca
     9121 gctgcatgtg gtccgcccag atccagtcgt gcatatggag cagccagcgc gccagtcgct
     9181 cctcgacatc atggagccgg ttgcaggccg cgctccgtgc cgcctggatc atctggatct
     9241 gggcatagcg aagcagcaaa tctcgaaccg ccggttcgtc ttccgcgagt cggagcaggt
     9301 ctggcaccga aatctggagt gcatcgccag ctccctggat cgtgacctcg tactggctga
     9361 tcgattcgcc caggagagtg gcgacgccga ccatcccttc gaacccggcc ggcactgctt
     9421 ccacgctgcg gccgtcatcc atccgtacga ccgccgagaa gacgaccccg gtcgagaacc
     9481 aggcgtgctc gattcggcca tcctgcggca ggatgacctg ccctagctcg aagtgtacgg
     9541 gccggcattt gcggcgaacg cgttcgttta cgtccggtgg caatagggca aatattcggt
     9601 tcgttcccgg ccccgggcgt ccgcgcggtc ctaccatgag cttctcccgt ctcgaacctg
     9661 gtggcgaacc ctacagcaat ctgtacggta ccgcactgtg caattcgagt cgctggtcaa
     9721 tacttcgcag accgaggcaa caaactccgg tgcttgtccg gcaacccgaa ggattccgcg
     9781 tgctgacccc tgacattctg gcggtgattt ccgatcgttg cgtggccgcc cggctgcgtg
     9841 gcgaccgtac cgtatcgatc gacctctttg atcccgaaat cgatgcccag ccctggtcgt
     9901 ggcacatcga tgtgagcctg atgtgcgact ggattgcgcg gcactggcgg ccggagggcc
     9961 cggccgagcc gtcagcatat cccgacgcgg tcagcttcct tgggcaacag cgtgacagtg
    10021 ccggaccgcg ctggtacgcc gcccaggaca tgattctcgg atacatcccc ggtactgttc
    10081 acggctgcta cgcaatttcg gtctggtcgg agctccccgg gttgctttcg gcagcgcccg
    10141 agccaggcat ggaatcctgg gcgtgacacc acgccgcatc gtgcggaacg acagcgtcat
    10201 gggcggacgc tggcatttcg aggggactga catagcgatc accgcggtcc gcgccgatgc
    10261 gattgccggc gtgggcaacc tcgcgcatat atgtcagcgc accgggctga ccgaagagga
    10321 gatcatgctt gccctgggct tcgagttccc ggaagtacgc gcgcctcagc tgtggtccca
    10381 atacgctgca gtcacgatcg cttgctcatg tggcgaggag tcgcatgcaa ccttctccgg
    10441 gttcgatcgc cgcgatattc tctgcgcatg tggccgcgtc tggtgtctcc gactcacact
    10501 cgatgcggac gtgcatcggg cggactagcg cctgctgacg acggtttttt tggcggcgtg
    10561 ggtggttgct cgcgctatcg cactaagtgc ccgccctcgg gagtggaggc tgggtccggg
    10621 agcagactcc ggtgtacgcg aaattgatca tctggcgact tggggcggac cacagagatg
    10681 tcgcagggta cgagcaattc ctgcgtgact ttgcgccgaa gtccctcgag cggctccgcc
    10741 agttcggcat gctcgatggc ttcatcattc gcctcacgca caatactgtg ctgacggtca
    10801 acctctatga gacagcggat ggcgcgtatt cggcctggca caacgtagtc agccaaccgg
    10861 aatacggcgc ggccggcgaa ctcgagttgc tctttcacct cattagccgg ggtgacgacc
    10921 tgccgatggt cggcgagttg gccctcgcgg ctgacgaaaa aagcacgacg aaaagcactg
    10981 acaaccggta acggaaccgc acgtttcccg cagtctgttg actcaggccg tcccggggcg
    11041 ggggacgcaa cccagctggg tgtcgcagat caggccgttg tcgcagcagt tggtgaccag
    11101 gctgataccg cccggatcgc ggatgtctgg gtagacgcag cactgagagc cgcgctggca
    11161 tagattgaac tcgaactccc ccttgcacac ccggaactcg ctgtggtcgt acccggttac
    11221 gtccttgcat atgacctgct gagtcgcgtc gtcgaacccg cagatctgct tgcccggaca
    11281 ctccaggttg ttgccggagc aggtgcggac cggggcggca gtggcctgac gggcccaggc
    11341 gccggcggca atcccaccga gcagggcacg aaacacggcc cggcgcgggc gaggggttgc
    11401 cagagacttg acgatcgcat cgaaacggga ttcgtccata gctgactcct tcttcatgcc
    11461 cacaaaggta ccgacgaagg cacgccgcat gttttggtgg attccaccac tctagggcgt
    11521 gctcagcgaa tgtcaagaga gagggatcag gggccagtga tcagcggtaa gtggtcagtg
    11581 gtcggtaggc tctccgctaa aacctaaaac cgaataccca actcgcgttg ccatgtgact
    11641 ttgcgcacct ttccccttat tcgttcactc aaccctccac ccccgtctga cacgtttttg
    11701 caatgtcggc cgcctggatt gggttaccgg tgaggcgacc gtgggcgcat cgttcgcact
    11761 ctgggcgccg ggagttgatg cccggccacc ttcgctttct caccctcgaa acccgcatgt
    11821 cgaggagggc tgagacatga aacgcgtcat cattgagcaa ttcggcgggc ctgaagtact
    11881 gaatgtcgtc gaggttgcca atccccgacc aggcccgggc gaggtgtcag tcagggtctt
    11941 ggccgccggg gtgtcattca ccgattccca gctacgtgcc ggcacatatc tcggcggtcc
    12001 caaaccgcca ttcacgcccg gttacgagct ggtcggggtg gtcgaggagc tgggtcccgg
    12061 ctgcacgaga ttgcgcgtgg gtgatcgggt cggcgcactg accgtctggg gcgccgacgc
    12121 cgagcgggtc tgcgtgccgg agaaatacgc ggtcgatgtg cccgaagatc tggacccggc
    12181 cgaggtcgtg agcctgatct tcccgtacat gaccgcgtac cagatgctgc accgcacggc
    12241 gagggtcaag tgcggcgaat ccgtgctggt ccacggcgcg gcgggcaggg tcggcacggc
    12301 ggcgctcgag ttgggtgcgg tggccgggct acgccagttc gggacctgtt ccgcgcgtga
    12361 tcgggcgcag gtcgaggccc tgggcgcggt cgcgatcgac taccgcagcg aagacttcct
    12421 cgaccgggtg cgcgagctga ccggcggcaa gggcgtggat gtcgtgatcg acgggatcgg
    12481 tggccgtgtc tcgctgcgct cattccgcgc gctgcgggtc ggcgaccgga tcaaaggcga
    12541 tcccggcgga cggctgatca tgatgggcaa ctacaacacc ctgcaacagg gtcacaagag
    12601 ctggcgcggg tggctggagt ggtggccggc gaccgcgatc gtctggactt ggggcacaat
    12661 ctcgccgcgc cgccgggtga tggggtaccg ggttcagaaa ttgcgcatcg accaccagga
    12721 ttggttcgcg gaggatttcc gcgtgctgct cgagttgctg cgtgcgggca agatccaccc
    12781 ggtcgtggcc gagcgcctgc cgctcatcga agcccgacat gcgcacgagc tgcttgagtc
    12841 cacggcggcg aaggggaagc tcgtcctcgt gccatagccg tccaggacga cagaattcgg
    12901 cgaccgatgt ctgtgcaggt tgcgccgaac aggtcgtgtc cagcggaatt cggcaggcat
    12961 ctcacgtgcg caacctgccg accgccgggc tcgacctgtt cggcgctttg caaacgtctt
    13021 tgggccttgg tctctcgtct ctggtctttg gttttccgtt ttccgtagcc atctaccaac
    13081 gaccaacgac caatcaccct ccaccatcca ccagcaaccc tccacccctt accatcgggc
    13141 gattgccgaa tccgcgtgct gaagtggcaa aatagacgcg caatttgccc tgcaaccgat
    13201 ccgcctttgc ttgtgctgtc catatcgagg ggaacctgct gccgtgacca tgactcgcca
    13261 attgccgccg accgatgacg ccgctgcttc actcatcggt cggcgcttga cccggcggca
    13321 gaccctcgca ctgctcggcg gcggactgac cggcggggcc atgctcggcg cggtcaccgg
    13381 cgatcccggc cgttctgtcg atgccgccca gatcactatc gaagccggcc cgtcggaggc
    13441 gcagtcgacc gggcgaccga ccattgctga cattgccgcc gcgctccagt gggacaccga
    13501 gcggatcttc cgcttcgtgg ccgaccacgt cgcctacgag ccgtatgtcg gggcgctgcg
    13561 cggagccgag ggcacgctgc gggccatggc cgggaacgcg gtcgaccagt ccctgctcct
    13621 ggccgcgctg cttgcggagg cagccgtgcc gtatcggttc gcgatcgggc agctctcggc
    13681 cgacgggcaa accgcgctcg aagcgcaact cgcgccgacg gtagaagagc tgagtgatcg
    13741 aaaactgcgc acgttccgca acccgaccga cccgcccgac acca
//
LOCUS       DATAOZ010000565         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9165890, whole genome shotgun sequence.
ACCESSION   DATAOZ010000565 DATAOZ010000000
VERSION     DATAOZ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9165890"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1961
                     /locus_tag="VHR64_14285"
     CDS             <1..1961
                     /locus_tag="VHR64_14285"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX3304052.1"
                     /translation="VQGTWPGNILKGSWSGSGFIRGSMITDGRSLDAGVVNPLVSPED
                     RANGKQIFLVHCAACHGKDGKGAGHAPSLAKPNFSIGSSDLALYKTLRDGIPGTAMAA
                     INLSMPERWQVIAFLRTLGDRARTEMSASSHAPSVNVGQEALLHARSRTDEWLTYSGA
                     LDGWRHSPLREITPANVLRLKLLWAHQFAAEEGTIQATPIVVNKTIFITEPPNNVVAL
                     EAGSGREIWRYVRRLPQELPVCCGRVNRGLAVFGDKLFMGTLDAKLVALDARTGEVKW
                     EIQVADPGEGFTITGAPLIANDAVIVGISGGEFGIRGFLAAYDPRTGQPRWRFNTIPG
                     PGETGHDSWANSAWETGGGPTWITGSFDPDLDLVYWGVGNPSPVYAGEARPGDNLFTN
                     SVIALDARTGKLVWHFQFTPHDQHDWDSNQTPVLADLMIDGAMRKVICWANRNGFYYV
                     LDRTTGRFLRGVPFVEVNWASGLDQRGRPILTDAAEVTTTGKLTRPWVAGGTNWLPPS
                     YDPATGIFLVHATEGASVYTNSPIEQVGRGQSGLVVGSGSTVADPPVNLVKALDAATG
                     ALKWAYVAPKQQNDLDWTYGGVLSTAGGITFSTSAGVIFALDTATGKELWRAGLGGRT
                     QATPISFSIDGRQVVVVAAGRTLFVFGL"
     gene            2137..3438
                     /locus_tag="VHR64_14290"
     CDS             2137..3438
                     /locus_tag="VHR64_14290"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX3304053.1"
                     /translation="MNIAVFGLGYVGAVSAACLSQAGHAVVGVDTNSAKADPINAGRS
                     PVVEPGVDEMIAVAVAAGRLRATTDARAAIRESELAIICVGTPSRANGDLDLTYVSRV
                     CEDIAKALRDKAGFTAIIIRSTVLPGTIRNLVVPTLESGSGKRAGQDFGVGFFPEFLR
                     ESTAVFDFNHPPKIVIAATDARTRAMLESLNAPLDAPVINADFEIAEMVKYADNTWHA
                     LKVIFANEIGSISKALGIDGGAVMDIFCQDRKLNISSKYLRPGFAFGGSCLPKDVRAL
                     TYKGRSLDLELPLINAIMPSNRSHIDRALQMVLDLKERNVGVLGLSFKAGTDDLRESP
                     VVELVERLLGKGHEVRIFDRNVNLSRLVGANRAYVYQHLPHIAKLMVDKVEEAIHHAG
                     TIVIGNSDRYFGEVVGRLNSSQRVVDLVRIDVEGAYNGICW"
BASE COUNT          661 a         1149 c         1152 g          631 t
ORIGIN      
        1 tcgttcaggg aacgtggcca ggcaacatcc tcaagggaag ctggtctggc agcggtttca
       61 tcaggggatc gatgattacc gacggtcgca gcctcgacgc gggggtggta aatccgctgg
      121 tcagtccgga agaccgggcc aacggcaaac agatctttct cgtccactgc gcggcctgtc
      181 atggcaagga tggcaagggc gccggacatg cgccgtcgtt agccaaaccg aatttcagca
      241 tcggctcgag cgatctcgca ctctacaaga cgctgcgcga cggaatcccc ggcacggcga
      301 tggccgcgat caatctctca atgcccgaac gttggcaggt catcgcgttc ttgcgcacgc
      361 ttggcgaccg tgccaggacc gagatgagcg cgagcagcca cgcgccgtcc gtcaacgtcg
      421 gtcaggaggc gctgttgcac gctcggagcc gaaccgatga atggctcacc tactctggtg
      481 ctctcgacgg ctggcgccac tctcctcttc gcgagatcac cccggccaac gtcttgcgat
      541 tgaaattgct ttgggcccat cagttcgccg ccgaagaagg cacgatccag gcgacgccga
      601 tcgtcgtcaa taagacgatt ttcatcacag aaccgccgaa taacgtcgtc gccctggagg
      661 ccggaagcgg tcgcgaaatc tggcgatacg tgcgccgcct gccgcaagag ctgccggtct
      721 gttgcgggcg tgtcaacaga gggctcgcgg tattcggcga caagctcttc atgggcacgc
      781 tggatgccaa gctggtcgcg ctcgatgcgc gcacgggcga agtcaaatgg gagatccagg
      841 ttgccgaccc gggcgaaggc ttcacgatca ccggagcgcc gctcatcgcg aacgatgcgg
      901 tgatcgtcgg catctccggc ggcgagtttg gcattcgcgg cttcctggcc gcatacgacc
      961 cgcggaccgg ccagccgcgc tggcgtttca acaccattcc cggcccgggg gaaaccgggc
     1021 acgacagctg ggcgaacagc gcttgggaga cgggcggcgg tccgacctgg atcaccggca
     1081 gcttcgaccc cgacctcgat ctggtctact ggggcgtcgg caatccgtcg ccggtctatg
     1141 ccggcgaggc gcggccgggc gacaatctgt tcacgaacag cgtgatcgcg ctcgatgcca
     1201 ggaccggcaa gctcgtctgg cactttcagt tcacgccgca cgaccagcac gactgggact
     1261 ccaatcagac gccggtgctc gccgacctga tgatcgacgg cgccatgcgc aaggtcatct
     1321 gctgggcgaa ccgcaacggc ttttactacg tcctcgatcg caccactggc aggttcctgc
     1381 gcggcgttcc gtttgtcgag gttaactggg cgagcggtct cgatcagagg ggacggccga
     1441 ttctgaccga tgctgccgaa gtcacgacga ccggcaagct gaccagaccc tgggtcgcag
     1501 gtggaacgaa ctggcttccg ccgtcctatg atcccgcgac tgggatcttt ctcgtccatg
     1561 cgaccgaagg cgcctcggtc tataccaact cgccgataga acaggttggg cgtggccaga
     1621 gcggccttgt tgtcgggagc ggctcgacag tcgcggatcc acccgtgaat ctcgtaaagg
     1681 ccctcgatgc cgcaaccggt gcactgaaat gggcgtacgt cgcccccaaa cagcagaacg
     1741 atctcgattg gacctacggc ggagtattgt cgacggccgg cggcatcacg ttctcgacat
     1801 cggctggcgt gatcttcgca ttggataccg cgactggcaa agaattatgg cgggcgggtc
     1861 ttggagggcg gacccaggca acgccgatct cgttctcgat cgatggccgt caggtcgtgg
     1921 tggtggcggc cggtcgcaca ctattcgtat tcggtctgta gctgatcccg ccggtccgcg
     1981 gcgccacggg ctgaccatcg gcgcgctgtc tcttgacgag gccgtatcta ccccggaagc
     2041 ggtacggcag gtaactatgc ccggcggtaa tgcgattggc gagcctgttt cgcggtgggt
     2101 ttgctgattt aatgaggcgc tgcagcagag gaagacatga atatcgcagt cttcggtctg
     2161 ggatacgtcg gggcggtttc tgctgcctgc ctttcgcagg ccgggcacgc cgtggtcggt
     2221 gtcgatacga actccgccaa ggcggacccg atcaacgctg gccggagccc ggtcgtcgag
     2281 ccaggcgtcg acgagatgat cgcggtcgcg gtcgcggcgg gccggctgcg cgcgaccacg
     2341 gatgcccgcg ccgccatccg cgagagcgag ttggcgatca tctgcgtcgg gaccccgagc
     2401 cgcgccaacg gcgatctcga cctcacgtac gtgagtcgtg tgtgcgaaga catcgcgaag
     2461 gccctgcgcg acaaggcggg cttcaccgca atcatcatcc gcagcaccgt cctgccaggg
     2521 accattcgca acctcgttgt cccgaccctg gaaagcggat ccggcaagcg ggccggccag
     2581 gacttcggcg tcggcttctt ccccgagttc ctgcgcgaga gcacggccgt cttcgatttc
     2641 aaccatccgc caaagatcgt aatcgcggcc acggatgccc gcacgcgcgc catgctcgag
     2701 tccctgaacg cgcccctcga tgcgccggtc atcaacgccg acttcgagat cgccgagatg
     2761 gtcaaatacg ccgacaacac ctggcacgcc ctgaaagtga tcttcgccaa cgagatcggc
     2821 agcatcagca aggccctcgg gatcgacggt ggcgcggtca tggacatctt ctgtcaggac
     2881 cggaagctca acatctcgtc aaaatacctt cgaccgggct tcgccttcgg cggttcctgc
     2941 ctgccgaagg acgtgcgcgc gctcacctac aagggccgga gcctcgacct ggaactgccg
     3001 ctgatcaacg cgatcatgcc gagcaaccgg agccatatcg accgcgcgct gcagatggtc
     3061 ctcgacctga aggagcgcaa tgtcggcgtg ctcgggctga gcttcaaggc cggcaccgac
     3121 gacctccggg aaagcccggt cgtcgagctc gtcgagcgac tgctcggcaa agggcacgag
     3181 gtccggatct ttgatcggaa cgtcaaccta agccggctgg tcggcgccaa ccgcgcctac
     3241 gtgtatcagc acctgccgca tatcgcgaag ctcatggtcg acaaggttga ggaggcaata
     3301 caccacgccg gcacgatcgt gatcggcaac agcgaccgct acttcggcga agtcgtcggc
     3361 cggcttaaca gctcgcaacg ggtggtcgac ctggtccgga tcgacgtcga gggtgcctac
     3421 aatggcatct gctggtgacc ggcgccgggt cctgatcatc gtccagaacc tgccggtgcc
     3481 gttcgaccgg cgggtctggc tcgaggcaac ctcgctgacc cgggcgggct acgcggtcag
     3541 cgtgatctgc ccgaaggcca agggctatga tgcatccttc gagcggctcg aag
//
LOCUS       DATAOZ010000566         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_226036, whole genome shotgun sequence.
ACCESSION   DATAOZ010000566 DATAOZ010000000
VERSION     DATAOZ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226036"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..204)
                     /locus_tag="VHR64_14295"
     tRNA            complement(118..204)
                     /locus_tag="VHR64_14295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Leu,seq:cag)
     gene            292..1248
                     /locus_tag="VHR64_14300"
     CDS             292..1248
                     /locus_tag="VHR64_14300"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX3304054.1"
                     /translation="MIDRKEELVTVFGGDGFIGRYVCELLLKAGARVRAATRNPKRGY
                     FIQPLAQVGQYGFVRADVADADSVRHAVRGASAVVNLVGVFGRSMQSVHVEGAGNVAE
                     AARRAGAGALVHVSAMGADPHSESNYGRTKGEGEAAVRKAYPKATIVRPSLVFGPEDQ
                     LTNRFAAMAGLPVLPVIAARTNFQPVYVRDLAQAIAQAALDPGKHGGKIYEIGGPARM
                     NMVELHRAIIAITGQTPDIVPLPDVVGAALSYLGFLPGAPLTRDQWMMLKRDNVTSGR
                     LPGLEAFGIEPTPLAAVGGEWLARFRGNRFAGGRRAHQTAAS"
     gene            1250..>1812
                     /locus_tag="VHR64_14305"
     CDS             1250..>1812
                     /locus_tag="VHR64_14305"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX3304055.1"
                     /translation="MPILLIAIILGIVEGVTEFLPVSSTGHLVLAGALLGYNDTQWNA
                     FNVIIQLGAILAIVVLYWRTFWAVLEGLFKGQAMSWRFVRNILIGFLPSAILGFLLID
                     RIEELLLKPVVVAWAFILGGIAILVIERVVKEHKIVGVAEMPLKTVIGVGLIQCLSMI
                     PGVSRSGATILGGLSLGVERRTAAEFS"
BASE COUNT          273 a          606 c          625 g          308 t
ORIGIN      
        1 cgcggatggc agcaattcgc aaagcggacc cggccccaga atcggcattg tctagccgga
       61 ggtgtttttg cgccgagacc cagacgctcg tcctctcggg agagtgcgtc gtgacggtgg
      121 tgcccaggag aggactcgaa cctccacgcc cttgcgagcg ccagcacctg aagctggtgc
      181 gtctaccaat tccgccacct gggcacggtc gtcattttgg cagggggcgg cgcttaaagg
      241 ggccgcgccc gccttgtcaa tctaggaagc gaggggctag tgcgccagcc catgatcgat
      301 cgcaaggaag agctcgtcac cgtctttggc ggcgacggat tcatcggccg ctacgtctgc
      361 gaactgctgt tgaaggcggg cgcccgggtc cgggccgcga cgcgcaatcc caagcgcggc
      421 tatttcatcc agccgctcgc ccaggtcggc cagtacggct tcgtccgggc cgacgtcgcc
      481 gatgccgaca gcgtccgcca cgccgtccgc ggggcgagcg cggtggtcaa tctggtcggc
      541 gtgttcggcc ggtcgatgca atcggtccac gtcgaaggcg cgggcaacgt cgccgaggcc
      601 gccaggcgcg ccggggccgg cgcactggtc catgtctcgg caatgggggc ggacccgcac
      661 tccgaatcga actacggtcg gaccaagggc gagggcgagg ccgccgtgcg caaggcctat
      721 cccaaggcga ccatcgtccg cccgtcgctg gtattcggcc cggaggacca gctcaccaac
      781 cggttcgcgg cgatggccgg cctgcccgtc ctgccggtga tcgcggcccg gacgaatttc
      841 cagccggtct atgtccgcga cctcgcccag gcgatcgccc aggccgcgct cgatccgggc
      901 aagcatggcg gcaagatcta cgagatcggc gggccggcgc ggatgaacat ggtcgaactt
      961 caccgggcga tcatcgccat caccgggcag acgcccgaca tcgtgccgtt gccggacgtg
     1021 gtcggggcgg ccttgtccta cctcggtttc ctgcccggag cgccgctgac ccgcgaccaa
     1081 tggatgatgc tgaagcgcga caacgtcacc tcgggcaggc tgccggggct cgaggcattc
     1141 gggatcgagc cgacgccgct ggcggcggtc ggcggcgaat ggctcgcccg cttccgcggc
     1201 aaccgcttcg ccggcggccg gcgggctcac cagacggccg ccagctgacg tgccgatcct
     1261 gctgatcgcg atcatcctcg gcatcgtcga gggggtcacc gagttcctgc cggtctcttc
     1321 gaccgggcat ctggtcctcg ccggcgcgct gctcggctac aacgacacgc agtggaacgc
     1381 gttcaacgtc atcatccagc tgggtgcgat cctcgccatc gtcgtgctct actggcggac
     1441 cttctgggcg gtgctggaag gcctgttcaa gggccaggcg atgtcctggc ggttcgtccg
     1501 caacatcctg atcggcttcc tgccgtcggc gatcctcggc ttcctgctga tcgaccggat
     1561 cgaggagctg ttgctcaaac cggtcgtcgt cgcctgggcc ttcatcctcg gcggaatcgc
     1621 gatcctggtg atcgagcggg tggtgaagga gcataagatc gtcggcgtcg ccgaaatgcc
     1681 gctgaagacc gtcatcggcg tcgggttgat ccagtgcctg tcgatgatcc cgggcgtcag
     1741 ccggtccgga gcgacgatcc tgggcggcct gtcattgggc gtcgaacggc ggacggcggc
     1801 ggagttcagc tt
//
LOCUS       DATAOZ010000567         5279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9166060, whole genome shotgun sequence.
ACCESSION   DATAOZ010000567 DATAOZ010000000
VERSION     DATAOZ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9166060"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VHR64_14310"
     CDS             complement(<1..1107)
                     /locus_tag="VHR64_14310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX3304056.1"
                     /translation="MNINAGDTAWVLTSAALVLLMTPALGFFYGGLVRKKNVLSTIMH
                     SFFILCLISVVWTLWGFSLAFGSDKGGLIGGLDFLGLRNITGDPLGNAPTIPAPAFVI
                     FQGAFAVITPALITGAFAERKRFKAFVLFSLLWSTFIYAPLAHWVWSPGGWIYEMGAL
                     DFAGGTVVHISSGISALVAALVIGKRVGFGQEEMEPHDLTMTVLGAGLLWFGWFGFNA
                     GSALAANGLAAMAFLVTNVSAAMGALTWLTVSWLKHGRPSVLGAAAGAVAGLVGITPA
                     AGYVSPASAIVIGFGAGFFCFFALELLKERLGIDDALDVFAVHGIGGIWGALATGLFA
                     EFAVNGTGADGLIFGNPAQLGKQIVAVGATLAFAG"
     gene            1438..3864
                     /locus_tag="VHR64_14315"
     CDS             1438..3864
                     /locus_tag="VHR64_14315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HEX3304057.1"
                     /translation="MASRSAAIDLAGLPSAPSAVEHAKSRAEGVLSSITVETLAWGGL
                     VVLAILTRFWELGAKALHHDESLHAYYSWIWAAGKGDYAHDPLMHGPLLFHLNALVYL
                     LFGASDATSRYAPATAGVLVVWLPYLLRGPKFLGRWGALATGALLLISPTIMYQSRYI
                     RHDIYTVAGALLMFICIVRYVAKPERNWIVTFGITTALMLANHEIIFAIIALFASFLY
                     GAVMFDRLRHWRYEHRDLVTSIIGLHVLAAFAAAALILFMPHTYVDRFLAIPWDSRGV
                     DSLPPTRQNQINYYQDMMSNWLIVGLLLVAVLFIVGLVYLLRSYGRITGTDEGWLDDA
                     HEGSPGAALHAVAADHRGLMIGGALFLLCFVGLFTTLFTHWYGIFSSTIATDGTLLYW
                     LGQHDVRRGEQPWFYFLLLLPQYEFLPITIGAALAALTWWRAIKTWITGETQDQRLFF
                     RLFLAWWFFGILAILSWAGEKMPWLIVHIALPGTILAGAAIGSLIERGIAMANERRIS
                     LLDAGIFAGLITAVIGWFFLAARLTYGTFNGTCANGDENCRRVTDSDLNNWWVLAIPP
                     AICLGLLAIAGVRRGWRQSALIAACAAVVVLGLLEVRVGWRLSYANPDIPTEMMVYTQ
                     TSTDVKRMVLESNQLSRELEAQGDGKILFDTGADGLSWPLYWYFRDNPNASPFGGTLN
                     ANTDAAVIFILANRSGTPQNSAVLSKYTGVTYAFRWHFPEELYRNFAIAPELPPGRSA
                     MASADSPHGPIDVIRSVVQSSASIFEAHGQQELFRMVMYRDIDDVLGYYEFQVYVRTD
                     LLPEFNQIRY"
     gene            3892..>5279
                     /locus_tag="VHR64_14320"
     CDS             3892..>5279
                     /locus_tag="VHR64_14320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3304058.1"
                     /translation="MAELSAEQRPDLVPRRGLSATINLAQVTWMSIAWVAVLTIGAGL
                     RLFQIGAASLSPTEGQKAFDAWSLVYGATEGPYREPSHVAPAGLLMRAFSFFLFGDSD
                     TTVRLISALIGVALIAMIWSIRDLLGDLRALGGATLVALSPTVVFASRTVNDEIVSLF
                     FGFLLFITVFRLGDADRTPGIGRAICLGGALAALIGSGPAGITVLLTLGIMFAVSPVI
                     SIDHGAVVQRAVRRLLDSRQLLLGALISLVIALVTLFTRFFSDPGAISGLGEVFADWG
                     RLIATESGTTPTQFFILSMLLYEIVAIVFAISAAVTEPRTEDGATGIDWSYPAVWFLA
                     SLVIFSFSSGRQPEHAVLVAFPLLLLGGFGLGDTVETMLATGDLRRRFGLLMLAVIGL
                     IIALISTLVLIGRVDTAINRNDALTQVLAAAIVALGPMVVLTYTMGDQLGRLTGWTPV
                     RAAAIAGLAIVLS"
BASE COUNT          898 a         1774 c         1591 g         1016 t
ORIGIN      
        1 gccggcgaac gccagcgtgg cgccgacagc cacgatctgc ttgccgagct gggccgggtt
       61 gccgaagatc agcccgtccg ctcccgtccc attgaccgcg aattcggcga acagaccggt
      121 ggcgagcgcc ccccagatgc cgccgatacc atggacggca aagacatcga gcgcgtcatc
      181 gatgccgagt cgctccttga gcagctcgag cgcgaagaag cagaagaagc cggccccgaa
      241 tccgatcacg atcgccgatg ccgggctgac ataccccgcg gccggcgtga tcccgaccag
      301 accggctacg gcccctgccg ccgcaccaag cacgctcggg cgaccatgct tgagccaact
      361 cacggtcagc cacgtcagcg cgcccatcgc ggccgacacg ttcgtgacca ggaaggccat
      421 cgcagcgagc ccgttcgctg ccaatgcgct gccggcattg aatccgaacc agccgaacca
      481 aagcagcccg gcgccgagca cggtcatggt cagatcgtgc ggctccatct cctcctggcc
      541 gaagccgacc cgcttcccga tcacgagcgc cgccaccaaa gccgagatgc cggacgagat
      601 gtggacgacg gtgccgccgg cgaagtcgag cgcgcccatc tcatagatcc aaccgccggg
      661 cgaccaaacc caatgtgcaa gaggcgcata gatgaaggtg gaccagagca gcgagaagag
      721 gacgaacgcc ttgaaccgct tccgctcggc aaaggcgccc gtgatcagcg ccggcgtgat
      781 caccgcgaat gcgccctgga agatcacgaa cgcgggcgcc gggatcgtcg gcgcgttccc
      841 aagcggatcg cccgtgatat tgcgcaggcc gaggaaatcg agcccgccga tcaggccgcc
      901 tttatccgac ccaaacgcga gggaaaagcc ccacagtgtc cagaccacgc tgatcaggca
      961 aaggatgaag aagctgtgca tgatggtcga gagcacattt ttcttgcgca ccagaccacc
     1021 atagaagaac cccagcgctg gagtcatcag cagcaccagt gctgccgagg ttagaaccca
     1081 agctgtgtcg ccagcgttga tgttcaatcg caccttcctc cataagaacg gaatgaccaa
     1141 cgagcgggtc gccgttcccg cccggttccc aatctatgaa ggcgcggttc gagtgaacac
     1201 gtgcagacgg gccgaattcg agtcgcattc ttgtgcaacg tgcacgaacc ggtagcagaa
     1261 atgtggcgcg cgcaatgaaa ctgccgcggc attgattacg ttcagtcgaa agaggctgtt
     1321 agaacggaca gggcccgatg gcagcgtcta tactcgcccg aacatccctg cgattcacgg
     1381 catgtgaggt tgatgcagcc gtgacgcgcg ggcattttgc agttttggga gtcgccgttg
     1441 gcatctcgta gcgcagcaat cgatctggcc ggcctgccat cagcaccgtc ggcggtcgag
     1501 cacgccaaat cgcgtgcgga aggcgtcctt tcatcgatca ccgtcgagac gctcgcctgg
     1561 ggcgggctgg tcgtgctggc gatcctgacc cggttctggg agctgggtgc gaaggccctc
     1621 caccacgatg agagcctgca cgcctattac tcgtggatct gggcagcggg caagggcgat
     1681 tacgcgcatg acccgctcat gcacggtccg ctcctgttcc acctgaatgc gctggtctac
     1741 ctgctcttcg gcgcgtcgga cgcaaccagc cggtacgctc ctgcgaccgc gggagtgctc
     1801 gtcgtctggc ttccatacct tctgcgcgga ccgaagttcc tggggcgctg gggcgcactg
     1861 gccaccggag cgctcttgct gatctcgccg acgatcatgt accagagccg gtatatccgc
     1921 cacgatatct acaccgtggc cggcgccctg ctgatgttca tctgcatcgt ccggtacgtc
     1981 gccaagcccg agcgcaattg gattgtcact ttcggcatca cgaccgcgct gatgctggcc
     2041 aatcacgaga tcattttcgc gatcatcgcc ctcttcgcat cgttcctgta cggcgcggtg
     2101 atgttcgatc gcctccgcca ctggcgatac gagcatcgcg acctcgtcac gtcgatcatc
     2161 gggttgcacg tcctggcggc atttgcggcg gcggcactta tcctcttcat gccgcatacc
     2221 tacgtggatc gattcctcgc gatcccatgg gacagccggg gcgtcgattc gctgccacca
     2281 acccggcaga accagatcaa ctactaccag gacatgatga gcaactggct gatcgtcggg
     2341 ctcctgctcg tcgccgtgct cttcattgtc ggcctcgttt acctgctccg ctcctacggc
     2401 cgcattactg gtactgatga aggctggctc gacgacgcgc acgaaggatc gcccggcgcc
     2461 gcacttcatg cggtcgcggc cgatcaccgc ggcctgatga ttggcggagc gctctttctg
     2521 ctctgcttcg tcgggctctt cacgacactc ttcacgcact ggtacggcat cttctcgtcg
     2581 acgatcgcga ccgatggcac gctcctctac tggctcggcc agcacgacgt ccgtcgcggc
     2641 gagcaaccgt ggttctattt cctcttgctc ctgccgcagt acgagttcct gccgatcacg
     2701 atcggcgctg ccctcgcggc gctgacctgg tggcgggcga tcaagacctg gatcaccggt
     2761 gaaacgcagg accagcggct cttcttccgt ctgttcctgg cgtggtggtt cttcgggatc
     2821 ctcgccatcc tgtcgtgggc aggcgaaaag atgccgtggc tgatcgtcca cattgcgctg
     2881 ccgggcacga tcctggccgg cgccgcgatc ggcagcttga tcgagcgcgg gatcgccatg
     2941 gccaacgagc gccggatcag cctgctcgat gcaggcatct tcgccgggtt gatcacggcc
     3001 gtgatcggat ggtttttcct ggctgccagg ctcacctacg gcacattcaa tggaacatgc
     3061 gcgaatggcg acgagaactg ccgccgcgtg accgatagcg atctcaacaa ctggtgggtg
     3121 ctcgcgatcc cgccggccat ctgcctcggc ttactcgcga tcgccggagt gcggcgaggc
     3181 tggcgacagt ctgcgttgat cgccgcctgc gcggccgtcg tggtgctggg gctgctcgaa
     3241 gtgcgggttg gctggcggct cagctatgcc aatcccgata taccgaccga gatgatggtc
     3301 tacacccaga cgtcgaccga cgtgaagcgc atggtgctcg aatcgaacca gctcagccgc
     3361 gagctcgagg cgcagggcga cggcaagatt ctcttcgaca ctggagccga cggcttatcc
     3421 tggcccctct actggtattt ccgtgacaac ccgaatgcct cgccgttcgg cggcacgctc
     3481 aatgccaaca ccgacgcggc agtcatcttc atcctggcca acaggtccgg aacgccgcag
     3541 aactctgcag ttctctcgaa gtacaccggc gtgacctacg ccttccggtg gcacttcccc
     3601 gaggagctct accgcaactt cgcgatcgcg cccgagctcc cccccggacg ctcggccatg
     3661 gcaagtgcgg atagcccgca cggcccgatc gatgtgatca ggtcggttgt ccagagctcg
     3721 gcttcgatct tcgaggcgca tgggcagcaa gaactgttcc gcatggtcat gtatcgcgat
     3781 atcgatgatg tcctgggcta ctacgagttc caggtttacg tccgcacgga tctcctgccg
     3841 gagttcaacc agatccggta ctagccggcc acaaggagtt cgatcccacc aatggcagag
     3901 ttatcggcag agcagcggcc cgacctggtt ccacgtcgcg gcctcagcgc cacgatcaac
     3961 cttgcgcaag ttacctggat gtcgatcgca tgggttgccg ttctgacgat cggcgcgggg
     4021 ctccgcctct ttcagatcgg cgccgcatcg ctctcgccga ccgaaggaca aaaggccttc
     4081 gatgcctggt cgctcgttta cggcgccacg gaaggaccct accgcgagcc gtcgcatgtc
     4141 gcgccggcgg gcctcctgat gcgggccttc tccttcttcc tgttcggtga ctcagacacc
     4201 actgttcggc tcatctcggc gctgatcggg gttgccctga tcgcgatgat ctggtccatt
     4261 cgcgacttgc tgggcgatct tcgcgccctc ggcggagcca cgctcgtcgc gttgtcgccg
     4321 acggtcgtct tcgcgagccg gaccgtcaac gacgagatcg tcagcctctt tttcgggttc
     4381 ctgctcttca tcacagtgtt ccgactcgga gacgctgacc gcacccccgg catcggccgc
     4441 gcgatctgcc tcggcggcgc actcgccgcc ctgatcggtt ccggaccggc cggtatcacg
     4501 gtgctcctga cgctcgggat catgtttgcg gtctcgccgg tgatcagcat cgaccacggc
     4561 gcggttgtcc agcgtgctgt ccggcgcctc ctggactccc gacaactcct tctcggcgcg
     4621 ctcatcagtc tggtaatcgc gctggtgacg ctcttcaccc gcttcttcag cgatcccggc
     4681 gcgatctccg gcctcggcga agtctttgcc gactggggac ggctgatcgc gaccgaatcc
     4741 ggcacgacac cgacgcagtt cttcatcctc tcgatgctgc tctatgaaat cgtcgccatc
     4801 gtcttcgcga tctcggctgc cgtcacggag cctcgcacgg aagacggcgc gaccggtatc
     4861 gactggtcgt atccggccgt ctggttcctc gcgtcgctgg tcatcttcag cttcagctcg
     4921 ggccgccagc ccgagcatgc ggtgctggtc gccttcccgc tgttgctctt gggcggcttc
     4981 ggactcggtg acacggtcga gaccatgctg gccaccggcg accttcggcg ccgttttggg
     5041 ctgctgatgc tggcggtcat cggcttgatc atcgcgctga tttcgacgct ggtcctgatc
     5101 ggccgggtcg atacggctat caatcgcaac gacgcgctga cccaggtcct ggctgccgcg
     5161 atcgtcgcgc tcggcccgat ggtcgtgctg acctacacga tgggcgacca gctcgggcgg
     5221 ctgaccggct ggacacctgt ccgtgccgcc gccattgccg ggctcgcgat cgtgctctc
//
LOCUS       DATAOZ010000568         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6270185, whole genome shotgun sequence.
ACCESSION   DATAOZ010000568 DATAOZ010000000
VERSION     DATAOZ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6270185"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VHR64_14325"
     CDS             <1..517
                     /locus_tag="VHR64_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304059.1"
                     /translation="HNEPGTAVALHPGTGLSGVAFLFRSRARIDHRNPDAGTELLSRV
                     YRDVGWRAQELLAAYRAAEDIYFDSVTRIRMSSWSRGRVTLIGDAASCVSLFGDGSSS
                     AMEGAATLAASLGTSAHDIHAALARYESVHRAIIRPRQRLVWIASHLLIPRSGVGITL
                     RDQALKIWGGR"
     gene            568..1221
                     /locus_tag="VHR64_14330"
     CDS             568..1221
                     /locus_tag="VHR64_14330"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX3304060.1"
                     /translation="MTDPAGAAPVVTVRGDAQLEGPPDLATLTLTLHASGDSAEQTRR
                     QLAEGSVTVAELLQGYEAAIERSSTSGVHVAPVFNRRTPTKIIGYQGTFTTQIVVSDF
                     DFLSPLVLALTTLSNSQLDGPWWSLRVDNPMYREVRLAAIADARRRADDYAAAFGTAV
                     AGLVEVSDLNTGFNGVPEMRAFSMAKGADDTPFEFKPATQTVSGQVTVRFSLKTVSL"
     gene            1311..>2886
                     /locus_tag="VHR64_14335"
     CDS             1311..>2886
                     /locus_tag="VHR64_14335"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX3304061.1"
                     /translation="MFAKVLVANRGEIAVRAFRAAYELGVRTVAVFPHEDRNAIHRIK
                     ADEAYRIGERGHPVRAYLDINEIMRAAKESDADAIYPGYGFLSENPDLARACAEAGIT
                     FIGPPANVLELAGNKVRALAAARAAGLPTLRSTPPSADLDELMAGAEAIGFPIFVKAV
                     AGGGGRGMRRVADPTQLRESVAAAMREAEGAFGDPTVFIEQAVGRPRHIEVQILADTH
                     GNTIHLFERDCSVQRRHQKVVEIAPAPHISEELREALCGDAVRFAESINYSCAGTVEF
                     LVETQGERAGQHVFIEMNPRIQVEHTVTEEITDVDLVQAQMRIAAGESLADLGLSQEV
                     IRINGAALQCRITTEDPANNFRPDTGTITAYRSAGGAGVRLDGGTVDIGVEISAYFDS
                     MLVKLICRGRDFESAVTRARRALAEFRIRGVSTNIPFLQAVLEDPDFMAGDVSTSFIE
                     ERPDLLTTHAPADRGTKLLRWLAEVTVNQPHGTAPTQLDPGLKRPVGIDLNVPSPQGS
                     RQRLLDLGPEAFAADLR"
BASE COUNT          504 a          941 c          899 g          542 t
ORIGIN      
        1 gcacaacgag cctggaacag cggtcgccct gcaccccggc actggattat cgggcgttgc
       61 cttcctgttc cggtcccgag cccggattga tcaccgcaac cctgatgcgg gcactgaact
      121 gctgagccgc gtctatcggg atgtcggctg gcgggcgcag gaactcctcg cggcctatcg
      181 tgcggctgaa gacatctatt tcgacagcgt gacacggatc cggatgtctt cctggtcgag
      241 aggccgcgtg accttgatcg gtgacgcggc ctcctgtgtc tccctattcg gcgatggctc
      301 cagctcagcg atggagggcg ccgcaacgct ggccgcatcg ctcggcacct ctgcgcacga
      361 catccatgcg gctctggccc gctacgagtc ggttcatcga gcgatcatcc gtccgcgaca
      421 gcgccttgtg tggatcgcgt cccacctcct gattccgaga tcaggagtgg gcatcacatt
      481 gcgtgatcag gccctcaaga tctggggcgg gcgataggtg tgcggctgct ccctctgtga
      541 cacctctgcg ggacgtaagg tcttgccatg accgatcccg ctggcgctgc tccggtggtg
      601 accgtacgcg gcgatgccca gctggaaggg ccgccggatc tcgcaacgct cacgctgaca
      661 ctgcacgctt caggtgactc agccgagcaa acgaggagac agctcgcaga gggttcggtc
      721 acagttgccg agctcctgca gggatatgaa gccgcaatcg aacgctccag caccagtggc
      781 gtccatgtcg ccccggtctt caatcggcgt acccctacca aaatcattgg ttaccagggc
      841 accttcacta cccagatcgt ggtgtctgac ttcgattttc tgtcaccgct ggtcctcgct
      901 ctcaccacgt tgagcaacag ccagctcgat ggcccctggt ggtcgttgcg tgttgataat
      961 ccgatgtatc gcgaggtgcg gctagccgcg atcgcggacg ctcggcgccg cgccgacgac
     1021 tatgcggcgg ccttcggcac agccgtggcg ggtctggtcg aggtgtcaga tttgaatacc
     1081 ggattcaacg gcgtacccga gatgcgcgcg ttttcgatgg ctaagggagc cgacgacacc
     1141 cccttcgagt tcaagccggc gacacagacg gtgtcgggcc aagtgaccgt gcgattcagc
     1201 ctgaagaccg tttcgctgtg actaagcctc gtcgtcaccg cttggcgcag ccgctcaggc
     1261 gctgggaagt tcgtctcgct gcggtaacaa acagtcgata gattcgatcc atgttcgcca
     1321 aggtgttggt tgccaatcgc ggcgagattg cggtccgcgc cttccgagcc gcctatgagc
     1381 tcggcgtgcg gaccgttgcc gtgttccccc acgaggaccg caacgcgatc caccggatca
     1441 aggcggacga ggcgtaccgg atcggcgaac gcggtcaccc cgtacgcgcc tacctggaca
     1501 tcaacgaaat catgcgtgcc gctaaggaat ccgatgcgga cgcgatctat cccggctacg
     1561 ggttcctgag tgagaatccc gatctcgccc gggcctgtgc ggaggcgggg attaccttca
     1621 tcggcccacc ggcgaacgtg ctcgagctcg ccggcaataa ggtccgcgca ctcgctgctg
     1681 cgcgagccgc aggcttgcca accctcagat cgacgccccc atcggcagac ctggacgagc
     1741 tgatggcggg ggcggaggcc attggcttcc cgatattcgt caaggccgtc gctggtggcg
     1801 gtggtcgggg catgagacgc gtcgcggatc cgacgcagct cagggaatcg gttgccgcgg
     1861 cgatgcgaga ggcagagggt gcgttcggcg atccaaccgt cttcatcgag caggccgtgg
     1921 gccggccgcg ccacatcgag gtgcagatcc tcgccgacac ccacggcaac accatccacc
     1981 tcttcgagcg cgactgctcc gtgcagcgcc gccaccaaaa agtcgtggag atcgctccgg
     2041 cgccgcacat ctccgaggag ttgcgcgagg cactctgcgg tgatgcggtg cgattcgccg
     2101 agtcgatcaa ctactcctgc gcgggcaccg ttgagttctt ggtcgagacc cagggcgagc
     2161 gcgccggcca gcatgtcttc atcgaaatga atccgcgcat ccaggtcgag cacaccgtca
     2221 ccgaagagat caccgatgtt gatcttgtgc aggcccagat gcgcattgcc gcgggcgaga
     2281 gtcttgctga tcttggtctg tcgcaggaag tgatcagaat caacggtgcc gcactgcagt
     2341 gccgtatcac cacagaagat ccggcgaaca acttccggcc cgataccggc accattaccg
     2401 cgtaccgttc ggcaggcgga gctggcgtac gcctggacgg cggcaccgtc gacattggtg
     2461 tggagatcag cgcctacttc gactcgatgc tggtcaagct gatctgccgc gggcgggatt
     2521 tcgaatctgc ggtcacccgg gctcggcgcg ccctggccga gttccggatt cgcggcgtca
     2581 gtaccaatat tccctttctc caggcggttc tggaggatcc cgacttcatg gccggcgacg
     2641 tctcaacctc tttcatcgag gagcgtcctg atctgctgac cacccacgcg ccggccgacc
     2701 gcgggaccaa gctgctgcgc tggctggcag aggtgacagt caaccagccg cacggcacgg
     2761 ctccgactca actcgatccc gggctcaaac gacccgttgg catcgatctc aatgtcccct
     2821 cgccccaggg ctcgcgacaa cgcctccttg atcttggtcc ggaagctttc gccgcggatc
     2881 tgcggc
//
LOCUS       DATAOZ010000569         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7590714, whole genome shotgun sequence.
ACCESSION   DATAOZ010000569 DATAOZ010000000
VERSION     DATAOZ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7590714"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VHR64_14340"
     CDS             complement(<1..1470)
                     /locus_tag="VHR64_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX3304062.1"
                     /translation="MSTTAPTAIATEPKTETRIASYLLTDYLERLGVEVVFGLCGHTV
                     IAFLDALGKSRIRFVSTRHEQVAAHAADGYARASGKPGVVLTHLGPGLTNACTGVANA
                     ALDSIPMVVIAGDVPSYYHGRHPHQEVNLHQDADQFQIYRPFCKRVYRVDRVTDLPRV
                     MERAFHLCQSGRPGPVLVDVPMDLFSEDLPVDAFQQIPAVMAPPTIDESVARHIVDAL
                     ASAERPLLYAGGGVMSARATTELAALAEALEVPVAHTLMGKGCLHADHPLLLGQSGFW
                     GTPISNDKCRSADLIVAIGTRLAEANSSSWDSRFTFSIPPTRLIHIDADEAEIGRNYP
                     TELGVVADAKFALAALAAAARGRTHRDRGRLREEIAGGRKAFAANWDHQWSSDQFPLR
                     PERILAELRKAVPEDGFIVTDVGWNKNGVGQQFPITVPGTFITPSGLATMGFGPSAVM
                     GVKMAHPERAAVALVGDGGFSTNPSVIATAMEAELPVVWL"
     gene            complement(1485..>1787)
                     /locus_tag="VHR64_14345"
     CDS             complement(1485..>1787)
                     /locus_tag="VHR64_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304063.1"
                     /translation="GHSCGIYSFDDDHIHRLALVAPVSRIMVRQVQSSSNAGTFTNGM
                     PMTSSMGCGTWGGNITNENISLKHYMNVTWVSRPTPEDRPSEQELFGEFYNSETFS"
BASE COUNT          332 a          568 c          560 g          327 t
ORIGIN      
        1 cagccacacg accggcagtt ccgcctccat tgcggtggcg atgaccgacg ggttcgtgct
       61 gaagccgccg tcgccgacca gtgccaccgc cgcccgctct ggatgcgcca tcttgacgcc
      121 catcaccgca gacggtccga agcccatcgt cgccagcccg ctgggtgtga tgaacgtgcc
      181 gggcaccgta atcgggaact gttggccgac gccgttcttg ttccaaccga catccgtcac
      241 gatgaagccg tcctccggca ctgccttgcg cagttccgca aggatccgtt ccggccgcaa
      301 gggaaactgg tcggagctcc actggtgatc ccagttcgcg gcgaaggcct tgcggcctcc
      361 ggcgatctcc tccctgagac gtccacgatc gcgatgcgtc ctcccccggg ccgcggcggc
      421 cagcgccgcg agcgcgaact tcgcgtccgc gacgaccccc agctctgtcg gatagttccg
      481 gccgatttcg gcttcatcgg cgtcgatgtg aatcaaacgc gtcggtggaa tcgagaacgt
      541 gaaacgtgaa tcccacgagc tcgagttggc ctcggccagg cgcgtgccga tcgcgacgat
      601 caaatcggcg ctgcggcact tatcgttcga aatcggcgtg ccccagaatc cggactgtcc
      661 gaggagcagc ggatggtcgg catgcaggca gcccttgccc atcaacgtgt gcgcaaccgg
      721 cacttcaagc gcctccgcga gagcggcaag ttccgtggtg gcgcgcgccg acatcacacc
      781 gcctccggca tagaggagcg gtcgctccgc cgacgcgagc gcgtcaacga tgtgcctggc
      841 gaccgactcg tcgatggtgg gcggtgccat cacggcagga atctgttgaa aggcgtcgac
      901 cggcaagtcc tcggagaaga gatccattgg cacatcgacg aggaccggac caggacgtcc
      961 gctctgacac agatgaaacg cgcgctccat cacgcgaggc aggtctgtga ctcgatcgac
     1021 gcggtagacg cgcttgcaga acggtcggta aatctggaac tgatcggcat cctgatgaag
     1081 attcacttcc tggtgtggat ggcggccgtg gtaatacgac ggtacgtcgc ccgcaatgac
     1141 caccatgggg attgaatcga gcgccgcatt ggccacgccg gtgcaagcgt tggtcaggcc
     1201 tggtccaagg tgcgtgagga caacgcctgg cttaccggat gctcgcgcgt acccgtcggc
     1261 cgcgtgcgcc gccacctgtt cgtgccgtgt cgacacaaac cgaatccggc tctttccgag
     1321 ggcgtcgagg aatgcgatga ccgtgtgccc gcagagaccg aagacgacct cgacgccaag
     1381 gcgctcgaga taatccgtca acaagtacga tgcaatccgc gtctcggtct tgggttcggt
     1441 cgcgattgca gtcggcgcag ttgtgctcat agggctacct gctgtcatga gaaggtctcg
     1501 ctgttgtaga actcgccgaa cagttcctgt tcggaaggcc gatcctctgg cgtcggccgg
     1561 ctgacccacg tgacgttcat gtagtgcttg agggaaatat tttcgttggt gatattaccg
     1621 ccccaggtgc cacagcccat gctggaggtc attggcatac cgttcgtaaa cgtccccgcg
     1681 ttcgagctcg attgcacctg gcgaaccatg atccggctca cgggcgcgac cagtgcgagc
     1741 cggtgaatgt ggtcatcgtc gaacgaatag atgccgcacg aatggcc
//
LOCUS       DATAOZ010000570         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3905738, whole genome shotgun sequence.
ACCESSION   DATAOZ010000570 DATAOZ010000000
VERSION     DATAOZ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3905738"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VHR64_14350"
     CDS             <1..1174
                     /locus_tag="VHR64_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304064.1"
                     /translation="EAAVLDAEDLLASVEFDATAGGSKNDGRDTTNTKLKPGVNGNVY
                     LDPTNGWAITWDDSVLTAEEWDPNDSGDVQGVQLATEGGNFMTVYVAEAKSIRACVTD
                     QVDQFDGSAFSDFEKVTDVDLPETGKGVRAGLYQGVFTSQSGDTSDIYLYSECRLLVV
                     DGEEVDGQFLIVNLISDVATYEDDLEAWSGALTSVEFDAASAKSDTSSKDDSGTKPGK
                     KKTPTADTTGSGISGSTYASAIGYQVSWDDSVFTGALLDDSNPDLGLSLSSDASIIQF
                     SAAGDPDADSCVEAETGVVENLSGMSTLKRSREDSPQGARDSASQLYAGTLTFDSGNE
                     ADVVVYIECRPMGELDNATLFLVIRMVGVKDAYADQLPLWQDILDSIQFFDPSGQG"
     gene            1286..>2153
                     /locus_tag="VHR64_14355"
     CDS             1286..>2153
                     /locus_tag="VHR64_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3304065.1"
                     /translation="MTKPLSIDTLAVRGGHYPKLGEGISPPIDLSTTFYLPADGAEGI
                     LAYGRSDVPAYRLFEQAVADVERAKFSIVFNSGTSGMIALADELKSGDRVVFTQDAYH
                     GFLVYAREELPKRGVTVDFIDFTDLTAVERAIPGAALAWVETPTNPHLHVTDLRAVGA
                     ICAQHGVRWIVDNTFSTPVLTRPIEYGAWAVLESVTKYLGGHTDVLLGAVATNDADLR
                     ERMEHQRARIGTQPDGFSCWLARRGMQTLPLRVRRQSETALRIATELQAHTKVERVYY
                     PGLPDDPGHEVAC"
BASE COUNT          399 a          711 c          677 g          366 t
ORIGIN      
        1 cgaggctgcg gtcctggacg cggaagatct cctcgcctcg gtcgaattcg atgcgaccgc
       61 cggcggcagc aagaacgacg gccgggatac caccaacacg aagctcaagc cgggcgtcaa
      121 cggcaatgtc tacctcgatc cgaccaacgg ctgggccatc acatgggatg actccgtgct
      181 caccgccgag gagtgggacc cgaacgactc gggcgatgtc cagggcgtcc agcttgcgac
      241 ggagggcggc aacttcatga ccgtctacgt cgccgaggcg aagagcatcc gcgcctgcgt
      301 gaccgatcag gtcgatcagt tcgatggctc cgccttcagc gacttcgaga aggtgaccga
      361 tgtcgatctg ccggagacgg gcaagggtgt ccgcgccggc ctctaccagg gcgtcttcac
      421 cagccagtcc ggcgacacca gcgatatcta cctctatagc gaatgccgcc tgctcgtagt
      481 cgatggtgag gaagtcgacg gccaattcct gatcgtcaac ctgatcagcg atgtcgcgac
      541 ctatgaggac gacctcgaag cgtggtccgg cgcactgaca tcggtcgagt tcgacgccgc
      601 gtcggccaag tccgacacaa gctcgaagga cgattccgga accaagccgg gcaagaagaa
      661 gaccccgacc gcagacacga cggggtccgg gatcagcggc agcacgtatg cctcggcaat
      721 cgggtaccag gtctcatggg atgactcggt cttcacgggt gcgctgctcg acgattccaa
      781 tcccgatctg ggcctgagcc tcagctcgga cgcgtcgatt atccagttct cggccgccgg
      841 cgatccggac gccgactcct gcgtcgaggc tgagaccggc gtggtcgaga acctgagcgg
      901 catgagcacg ctcaagcgca gccgggagga cagcccgcaa ggcgctcgcg attccgcgtc
      961 gcaactctac gcgggcacac tcacattcga cagcggcaac gaggcggatg tcgtggtcta
     1021 catcgagtgc cggccgatgg gcgagctcga caacgccacg ctcttcctcg tgatccggat
     1081 ggtcggagtc aaggatgcct acgcggatca actgcccctc tggcaggaca tcctcgattc
     1141 gatccagttc ttcgatccgt ccgggcaggg ctgaacaaaa cacgcaatgc ggggccggtc
     1201 tatcgaccgg ccccgttttc tttgccgcag cgaccgtata ctcccccgta cagtgcgacc
     1261 acccaccgca agccagggga aatccatgac caagccgttg tcgatcgata cgctcgccgt
     1321 gcgtggcggg cattacccca agctcggcga ggggatatcg ccgccgatcg acctctccac
     1381 gaccttctat ctccctgccg acggcgcaga ggggatcctc gcctacgggc gctccgatgt
     1441 gccggcctac cgcctcttcg agcaggcggt ggccgatgtc gaacgcgcga aattctcgat
     1501 cgtcttcaac tctggcacct cgggcatgat cgccctggcg gatgagttga agtcgggtga
     1561 tcgcgtcgtc ttcacgcagg acgcctatca cggttttctc gtctacgccc gtgaggagct
     1621 cccgaagcgc ggcgtgacgg tcgacttcat cgatttcacc gatctgacag ccgtcgagcg
     1681 agcgattccc ggcgcggccc tggcctgggt cgagacgccg accaatccgc acctgcacgt
     1741 gaccgatctg cgggcggtgg gcgcgatctg cgcgcagcat ggcgtgcgct ggatcgtcga
     1801 caacaccttt tcgacacccg tcctgacccg accaatcgag tacggcgcgt gggcagtcct
     1861 ggagagcgtg accaagtacc tcggcgggca caccgatgtc ctgctcggcg cggtcgcgac
     1921 caatgatgct gatctgcgcg agcggatgga gcaccagcgg gcgcgaatcg gcacacagcc
     1981 cgatggcttt tcatgttggc ttgcccggcg cggcatgcag acattgccgt tgcgagtccg
     2041 ccgccagtcg gagacggccc tgcgcattgc gaccgagttg caggcgcaca ccaaggtcga
     2101 gcgggtctat tatccgggcc tgccggacga tcccggccac gaggtcgcct gcc
//
LOCUS       DATAOZ010000571        15902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4166357, whole genome shotgun sequence.
ACCESSION   DATAOZ010000571 DATAOZ010000000
VERSION     DATAOZ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15902
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4166357"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VHR64_14360"
     CDS             <1..1512
                     /locus_tag="VHR64_14360"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX3304066.1"
                     /translation="ILLTATESRTLKATADQVRVAITLESNDKLRAQLASMFGFAHEP
                     HLSEDVHEVVDEHPSWPTPDSRLVPSRVSIPMGDLTTSKPWRDEPVDASSGISVPPKP
                     VPRPEFALEPRTGSIAKQAPEARAKTKPATIVGITVGILVALLVAAVLSVVLRTAEVT
                     VRTPRQPVSTQLVVGYSTNGSDVPGATITLPATSEQFTVPYSAKVPATGTLDNQGGKA
                     GGTVQLRNISGKAVTFPAGTRLQMADGVVYLTSADVNVPKGDTDKPGQAEVSIQAEKP
                     GTVGNREAGTFTGQIAEAPGVYFGNPGAAITGGTDVLIKVVTDQDLANARDAAIAAMS
                     QSAQSYQLPDGRIVIPSTVQSAGEPSVQTDHATGEQVDAFTVTGQATYQAQTISPADL
                     PAQMQADVRNQLVTQVPSGYALTDDPIAFASPVEQAAGSGLMTVTVTVDSAQQLSPDL
                     IDQIKNAASGKSQSDAQQAIGEIEGVEVIGISVSPALFDKTLPGAGKIDVKAE"
     gene            1530..1949
                     /gene="ruvX"
                     /locus_tag="VHR64_14365"
     CDS             1530..1949
                     /gene="ruvX"
                     /locus_tag="VHR64_14365"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX3304067.1"
                     /translation="MGLDIGSRRIGVAVGDELGIVATPVGFVQPGPSDRTAFADLIRR
                     HRVTRLVVGIPRSMSGKEGPQAKEVRSYATALAADLDVPLTFWDERLTTVIAERSLIS
                     GGRTRQQRKGEIDAAAAAVMLQGYLDHQAMQRRRESP"
     gene            2008..2301
                     /locus_tag="VHR64_14370"
     CDS             2008..2301
                     /locus_tag="VHR64_14370"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX3304068.1"
                     /translation="MKKLVIAVVQNDDADNVVDALLDQDFRATRLASTGGFLRRGNTT
                     LLVGADEDQVDRVLGLIRANAHAAGLPADAQPGLTPPAAATVFVLDLDEHQRI"
     gene            complement(2298..3089)
                     /locus_tag="VHR64_14375"
     CDS             complement(2298..3089)
                     /locus_tag="VHR64_14375"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX3304069.1"
                     /translation="MSQIPTTRPNLHDRIELAVSLIRGASSVVALTGAGISTESGIPD
                     YRGPGGVWETQKPPTIGDYMENEETRKAFWRRRLTDYPLLAARKSNAGHRALVELERR
                     GTLLAVITQNIDGLHQKAGTSPERVIELHGSAHRIRCVNCGRLWDANQIQQRLIAGEE
                     DPRCQVCGGILRSGTVLFGEPLPREELDKSVAVSNACDVMLVVGSSLVVNPAARLPEI
                     ARRRGASVIMLNRTETPLDQIADVQILGEAGPTLSDLIGRLDSGR"
     gene            3283..3516
                     /locus_tag="VHR64_14380"
     CDS             3283..3516
                     /locus_tag="VHR64_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304070.1"
                     /translation="MIMTSYTRLVVQSHQDDCVELFAGFSRRLTRRSPKLPKSEAYRE
                     HDGTVVRLGPPVPISAEVTELVHVTSRQIHGAA"
     gene            3482..3829
                     /locus_tag="VHR64_14385"
     CDS             3482..3829
                     /locus_tag="VHR64_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304071.1"
                     /translation="MSHRDKSTAPLEQDPLTPSEERARIYAVKGYLRARDGQYDQAQQ
                     HFAQAAGLDPELDLTTVPRFWDLPRAGHDCAVAALEQVGRNREASRLLAIINRRFRPK
                     LVNQRPRPIEPIT"
     gene            3956..5476
                     /locus_tag="VHR64_14390"
     CDS             3956..5476
                     /locus_tag="VHR64_14390"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304072.1"
                     /translation="MPAHRLTVRPLRFALPFLVVLGLLVPAFATRTFGQSSPARGTAQ
                     VIAQGLTAPPADKVAWRVIEQPIPKRLDARPSNRMQASTGFLLADETPIFVADQRTKL
                     RSRLAPGEAQFVPTGANQTWASLGEDAGTAYSLELVDREVAKVSPEAKILYRSGSFSM
                     KPGDYDLDLIRDDVKSGERAKIEDGSFPVLVLVTSGQVEVTSTNAKEPVRLKEGQAIA
                     LRGELTIRARGKDSTYVAAIMGDAVSGGEAVPTVEPTSTPKPKKTSTPEPEATKKPKK
                     TKTPKAEPTETPGVGDGASVRIAVRLCREGMTYFAINPQACERADGDYQLTLVAPNGR
                     RLHLSDASRVEPNFVRWSGLKPGDYELVVGKLPDGYKSYSLDGFLCCSTNEGYSITVR
                     KNQLIDGTLYLFTEDWGVGAPPAPTPIPPAPQPGNPAPGADSDGDGLSDELETDVFGT
                     SPFLVDSDGDTIPDGVEAFGSNGYLTAPALPDTDFDGVMDNVEIQKGTDPLDPNSR"
     gene            5960..6823
                     /locus_tag="VHR64_14395"
     CDS             5960..6823
                     /locus_tag="VHR64_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304073.1"
                     /translation="MQVAALVCRAVGEACQTKRDCCPEMAASCVDGVCACPHRRALDP
                     QVMVCNGPCAIPGEQCDEQTPCCDGAGMCIRGVCTCAKDELVCGAQCEPAEGNFQRNN
                     RNCGGRGLICEADQVCCEGLCIDRTALLDDPDHCGSCCTACRAGQICCNGRCEEPFSD
                     SGDPEICCDTGSICVGADGSEICCGPGDACTSDGCCPQDRVCGEECCGDGTFCYCGAC
                     VTDLATCESDADCCAGSCTGGFCCPSESICHDFAGNPVCCGGLSLCVEDTNVCVAREY
                     GSLDRPVRWVA"
     gene            complement(6824..7804)
                     /locus_tag="VHR64_14400"
     CDS             complement(6824..7804)
                     /locus_tag="VHR64_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX3304074.1"
                     /translation="MVDFRTAHPWVAERRKSVRFAVQAFPLPDDTNPTESVIEAGLLA
                     EQVGLDGFFIGDHPGYHIEPWLHLANLAARTSNVQLGSVVNCVFHRQPSMLARMAADL
                     DRISNGRVMLGLGIGWNEPEFAQLGVPFRPVPERQRGLEEALQIIDGMFGPEPITFTG
                     ESWHVLNAHITSPPVQRPRPPILVAGAGEKTLRQVVRWADIANFGASKNTGNVTSDEA
                     TRKKLELIDQLCDEEGRDPATLLKSHFTSWLMLAETEESAKAKLDRYYPDGLTEEQHR
                     TRIFGSPQKVIDYFQGLIGIGFEYFVIQIQDSRDLETIRLLGETVAPSFA"
     gene            7942..8607
                     /locus_tag="VHR64_14405"
     CDS             7942..8607
                     /locus_tag="VHR64_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX3304075.1"
                     /translation="MQIKALIFDMDGLLVDTEPLAYQAMDAFLAKHEVERRQDIHDQM
                     LGRRLPEAIAIVKEGYGLTHPLDDLIADYTIMRRDILVGNVKPMPGAMEVIRFGHDAG
                     LKTGLASSGLRDQVTLSLNEAGMRGMFEVEVTGDDVTRGKPAPELFLKAAEGLGIDPV
                     NCVVFEDAPAGVAAAVNAGMRAVAVPNQHSREMEFAVKPDAVLTSLHDAIPWLKRHGV
                     GRK"
     gene            8613..9896
                     /locus_tag="VHR64_14410"
     CDS             8613..9896
                     /locus_tag="VHR64_14410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3304076.1"
                     /translation="MERHVYDLAIVGAGIAGLAAARRAIDLGLSVVVLEATDQIGGRI
                     RTVSRPDGSIWDAGAHWLLQPAVNPLVREAERLGVRFRRQWRAHDGYWLDGQWLSELE
                     SDAVWLDIDDAEDLARELSAADEDVAFAELIDPESGALAMIETILTQQYGEHPGRISA
                     IDRSRYGVFEGDWPVIGGFGRFVEQLFADVPVRPGSPVSVIDWGDEPVRLDVFGDLVE
                     ARKVLVTVSTGVLLSGQVLFVPELPADKVAAISSLPMAAQDKVAFRIDPTLLDCTADS
                     IQYTRAGDSIVIFNVLPGGQPLVVAYISGYLCRDPERQGKDALIGAVIEQFEEVYGPE
                     VAATVSDPEAAIWGTHPYVGGAWAYPEPGALTARAVLTEPVDGRLWFAGEATSQNAAG
                     TVHGAYESGLNVAELIAESLGLKVETPLGGDNRLI"
     gene            9932..11179
                     /locus_tag="VHR64_14415"
     CDS             9932..11179
                     /locus_tag="VHR64_14415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3304077.1"
                     /translation="MSSQRAVDLVIIGAGAAGLGAARRAGELGLSYNVCEAMDRIGGR
                     AHTDSKTFGTPWDRGCHWLHSGSVNPFAKLADQYGFHYESTPPVRRSFDGSRWISGDE
                     QAELEKHVYGDLWGAIERAGKAGKDISAAEVVDMHDPWIAILRTALAGEWSVDIPDVS
                     TGDDVAYRDTHENWPVREGYGALVARHAEGIPVELSTPVTKITWDGPGVEVETGAGTI
                     SARAVVITVSTRIIQDDVIQFSPRLPDWKREAYGAITLGNANKVSFKIDKNLLGDAHN
                     TAWIRATPDQGMWFQMRAFDRDMANGYIAGALGEAAEAEGEAALIACGREALKSAYGS
                     DIVKAIQVEACTTWQSEPWIRGAYGAAQPGKAHLRKDLATPIDDRLYFAGESTSLDFF
                     STCHGAHLTGIAAVESAAKAVGR"
     gene            11185..11820
                     /locus_tag="VHR64_14420"
     CDS             11185..11820
                     /locus_tag="VHR64_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX3304078.1"
                     /translation="MEIILATTNRAKVRALAERVGAGIVVAPLPSETGERHIPLKLED
                     GVDATAIARTKASWYSRHIPKMFTAASDGGILIPALGRDWNPARTDRISGADPREATR
                     VEALLARARHLVGPDRQIGWTEALAVARDGEILAEWVAHSPPGELAVDLPDDFDPEAG
                     FWVPYVWRCPEYGGRRLSELTEHERLARRDHWAILGENLTEWALDQTGSTS"
     gene            complement(11828..12850)
                     /locus_tag="VHR64_14425"
     CDS             complement(11828..12850)
                     /locus_tag="VHR64_14425"
                     /inference="COORDINATES: protein motif:HMM:NF023354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent oxidative N-demethylase subunit
                     alpha family protein"
                     /protein_id="HEX3304079.1"
                     /translation="MPPLSAPYQPFLVRDDFIVRADLFPLPGTYNGRRETRHFELDNQ
                     FDLFVTEKLAALARDPARYRCLDPDESDALAEVYWRIFRILAAEHPEYVQIDGETIHM
                     RLPGLTIDAGDRQHVGVTVRSDASEIGRAVARWIDAQSGLDRFGDALALACQEDIVIM
                     RLLPDGRHRAESLHVLLPSAWNPREKYQQSFGTIHEPVADSARLVASADNVMKAIVTK
                     GPYIRFGLSLTTLPSLDNHSDLPKPWDPAWIADPDLLASKVSVRIERQTTYPMPDLGR
                     ALFTVRIYSTSLPELAGNRPDLISRLATILRSASPAVLAYKGIAEYALPVAAWCEARR
                     DTSRQT"
     gene            complement(12866..13729)
                     /locus_tag="VHR64_14430"
     CDS             complement(12866..13729)
                     /locus_tag="VHR64_14430"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX3304080.1"
                     /translation="MKLLTYKADGELKSGIATDKGVIDVASAGLALGIDAPTTVAGLA
                     DGLDKLQSLVDKIEGSEAWLHDESKLTLGPCVADPGKIICVGLNYAKHAAESNMPIPT
                     NPVLFSKFNNSLAGHNEDVPLIDDVASQFDYEVELCVVIGKTVRNVSEADALSYVAGY
                     CTANDLSVRDLQMRTSQWLLGKTSDKFFPIGPYLVTADEIGDPQTLRLTCKVNGETRQ
                     DSNTSDMVFTVAQLVSYCSKYLTLEAGDLITTGTPEGVAMGMPNQPWLKKGDVVEVEV
                     EKLGKLTNTMV"
     gene            complement(13800..14960)
                     /locus_tag="VHR64_14435"
     CDS             complement(13800..14960)
                     /locus_tag="VHR64_14435"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX3304081.1"
                     /translation="MRSISHLAGSIERSGIRDLMDAAREMGGEIIHLEVGEPSFTTPA
                     HIIEQAFEATAAGATRYTQSGGILPLRQAIADRYSAKWGKEVTPNMVMASHGGVNAIN
                     STIFALIDRGDEVLVPDPGWPNYRQILNLVGGVSVLYPMSVENGYLPDPEALEKLITP
                     KTKMIITCNPGNPTGAVWPEETVKAVVELAAKHDLFVLSDEIYEELVFDGEHVPAAAF
                     DDAGRVITVSGFSKTYAMTGWRLGWAIADVSLLQAVNKMLEPLISCPSSVSQQAGIAA
                     LTGTQQPVEVMCNAYRKRRDLAAGILEPAGLLPVRPKGAFYALVDLRETGMNGRDLAM
                     ALLKEKRVATAPGDTFGKVLADGFVRISLASSDEDVAEGCRRLISFRDEHRK"
     gene            15111..15701
                     /locus_tag="VHR64_14440"
     CDS             15111..15701
                     /locus_tag="VHR64_14440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3304082.1"
                     /translation="MVEYQPHAIADYRPVLSAESDEILAARAATDAAAFAEIYNRYVA
                     RIDGYCRQRLHNADLAEDATHQIFLRALESLRKHPVDNVAAWLFTIAHNECIDRYRRH
                     RAHVPIEMAAEMEGFDRSPEDQAVARSMANELRDLLPLLSEDQRRVLELRLAGLSSGE
                     IEIVLGKSRSWVGVIQHRAVLRLRTLMVVQAEPEER"
     gene            15702..>15902
                     /locus_tag="VHR64_14445"
     CDS             15702..>15902
                     /locus_tag="VHR64_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304083.1"
                     /translation="MRWLRGMNHQNESDALNEFWDEVVQRSPATPLTRDPVPTELGAI
                     VRQLHAEQDARQQRPAYADRQLA"
BASE COUNT         2910 a         4980 c         5182 g         2830 t
ORIGIN      
        1 attctgctca cggcgactga gtcccgcacg ctcaaggcga ctgccgacca ggttcgcgta
       61 gccatcacgc tcgagtcgaa cgacaagctc cgcgcccagc tcgcgagcat gttcggtttc
      121 gcccacgagc cgcacctcag cgaagacgtg catgaggtcg tcgacgagca tccctcctgg
      181 ccgacgccgg acagccgcct ggttccctcc cgcgtctcga ttccaatggg cgacctgacg
      241 acatcaaagc cgtggcgaga tgagcccgtg gacgcatcga gcggcatctc cgtccctcct
      301 aagccggtgc cacgaccgga gttcgcgctc gagccacgga ccgggtcgat cgcgaagcag
      361 gcgccggaag ctcgagccaa gaccaagccg gcaacgatcg tcggaatcac ggtcgggatc
      421 ctggtagcac ttctggttgc ggcggtgctg tccgtggtgc tgcggactgc agaggtcacg
      481 gtgcggacgc cgcggcagcc agtctcgacg cagctcgtcg tgggctactc gaccaatggc
      541 tcggacgtcc ccggggcgac gatcaccctg ccggccacgt cggagcaatt cacggtgccc
      601 tattcggcca aggtcccagc cactggtacg ctcgataacc agggcgggaa ggccggcggg
      661 acggttcaat tgcgcaatat cagcggcaaa gcggtcacat tcccggccgg aacacgcctc
      721 cagatggctg acggcgtcgt ctatctgaca agcgctgacg tcaacgtgcc gaagggagac
      781 acggacaagc cgggccaagc cgaggtcagc atccaggccg agaagcccgg caccgtcgga
      841 aatcgcgaag cgggcacgtt caccggccag atcgcggagg ctcccggcgt ttacttcggc
      901 aacccgggcg ccgcgatcac gggcggaacc gatgtcctga tcaaggtcgt gactgatcag
      961 gatcttgcaa acgcgcgtga tgccgcgatc gccgccatga gccagtcggc gcagagttat
     1021 cagctcccgg acggccggat cgtgatccca tcgaccgttc aatcagccgg cgagccatct
     1081 gtccagactg atcacgcgac cggtgagcaa gtcgatgcgt tcaccgtcac cggtcaagcg
     1141 acataccagg cgcagaccat cagcccagcc gatctgcccg cgcagatgca ggcggacgtg
     1201 cggaaccaac tggtgactca ggttccgtcc ggctacgcgc ttacggacga cccgatcgcg
     1261 tttgccagtc cggtcgagca ggcagctggt tcgggactga tgaccgtgac cgttacagtt
     1321 gactctgccc agcagctcag ccccgatctg atcgaccaga tcaagaacgc agccagcggc
     1381 aagtcgcaat ctgacgcaca gcaggcgatc ggcgaaatcg agggtgtcga ggtcatcggc
     1441 atctcggtca gtccggcgct cttcgacaag acgctcccgg gcgcgggcaa gatcgatgta
     1501 aaggctgaat gatagtgact gagcggacaa tgggattgga catcggatcg cgccggattg
     1561 gcgtcgcggt tggcgacgag ctcggtatcg tggcaactcc ggtcggattc gtgcagcccg
     1621 gtccgagcga ccgcaccgcc ttcgccgacc tgatccggcg gcatcgggtc actcgcctgg
     1681 tcgtgggcat cccgcgctcg atgtccggca aggagggccc gcaggcaaaa gaggtgcggt
     1741 cgtatgccac cgctcttgcc gccgacctgg acgtaccgct gaccttttgg gacgagcggc
     1801 tgacgacggt gatcgcggag cgaagcctga tctcgggcgg tcgcacgcgc cagcagcgca
     1861 agggcgagat tgatgccgcc gcagctgccg tcatgttgca gggatacctc gatcaccagg
     1921 cgatgcagcg ccgtcgggaa agcccgtagc tctcggctat actcggtcga attgcagaac
     1981 ccggagttgg ggtggggagt agcacgcatg aagaaactcg tcatagccgt ggtccagaac
     2041 gacgatgccg ataacgtggt cgatgccctg ctcgatcagg atttccgggc cacgcggctc
     2101 gccagtactg gcggcttcct gcgccgtggc aacaccacac tgctggtcgg cgcggacgag
     2161 gatcaggtcg atcgcgtgct cggattgatc cgggccaatg cccacgccgc aggcctgccc
     2221 gcagatgccc aacccgggct cacccctcct gccgccgcga ccgtcttcgt cctcgatctc
     2281 gacgaacacc agcgcatcta gcgaccgcta tccagcctgc cgatcaggtc gctcagggtt
     2341 ggcccagcct caccgagaat ctgaacatcg gcaatctggt cgagcggtgt ctcggtccgg
     2401 ttgagcatga tcacgctcgc gccgcgccgc cgggcgattt ccggcaatcg ggcggccggg
     2461 ttgacaacca gtgacgagcc cacgaccagc atcacgtcgc aggcattgct gaccgcgacc
     2521 gacttgtcga gctcctcccg gggcaatggt tctccaaaga gcactgtgcc ggatctcagg
     2581 attccgccgc agacctggca ccggggatcc tcctcgccgg cgatcaaccg ctgctggatc
     2641 tgattggcat cccacaggcg cccgcaattg acgcagcgaa tccgatgcgc gctgccatgc
     2701 agctcgatca cgcgctcggg cgaggtgcct gccttctggt gcaggccgtc aatgttttgg
     2761 gtgatcacag caagcagggt gccgcgccgc tcaagctcca ccagcgcgcg atgaccggca
     2821 ttcgacttac gcgcggccag caatgggtaa tcggtaaggc ggcggcgcca gaacgccttg
     2881 cgcgtctcct cgttctccat gtagtcaccg atggtcggcg gcttctgcgt ctcccagaca
     2941 ccacccgggc cgcggtagtc cggaataccc gattcagtgc tgatccccgc gccggtcaac
     3001 gccacgaccg acgatgcgcc gcgaatcagg cttacggcga gctcgatgcg atcgtgcaaa
     3061 ttcggccggg tagtggggat ttggctcatt cgtatcctct atccggcacg atcagtacca
     3121 ttttgccggc gctccgggta ttgtgtacgt acaccagcga tgctacgatt tgtgccccgt
     3181 agtcaaatac tctcatgcac caggggcgtc cattcccgct tgccattcca cccctgatgc
     3241 gtatacggtg cgcgtcaatt gagacgcgcg gggagcgcgg gcatgatcat gacctcttat
     3301 acgcgcctgg tcgttcagtc gcatcaggac gattgcgtag agctgtttgc tggtttctcg
     3361 cgccggctga cccgccgatc gcctaagctc cccaaatccg aagcctaccg cgaacacgac
     3421 ggtacagtgg tccgcctggg cccgccggtg ccgatcagcg cggaggtgac tgagttggtg
     3481 catgtcacat cgcgacaaat ccacggcgcc gcttgagcaa gatccgttga ctccgtccga
     3541 ggaacgcgcg cgcatctatg cggtgaaggg atacctgcgg gcgcgggacg gccagtatga
     3601 tcaggctcag cagcacttcg cccaagcggc cggtcttgac cccgagctcg acttgacgac
     3661 tgtgccgcga ttctgggatc tgccacgggc cgggcatgac tgcgcggtcg ccgcgctcga
     3721 gcaggtaggt cgcaatcggg aggcttcgcg ccttctggcg atcatcaatc gccgatttcg
     3781 gccgaagctc gtcaaccaac ggccgcgccc aatcgaaccc atcacctagc agattgctgc
     3841 cgccaaaggc atcagaaccg tcgaatggga tacgcttggt cgttgcattg atccgacgcg
     3901 cccggttgtc ggccctggct ggacttcacc gtgcgcgtga cctgatgagg tgaccatgcc
     3961 cgcacaccgc ttgacggtga ggccactccg cttcgcactt ccattccttg tcgtgctggg
     4021 attgctggtc ccggccttcg cgactcgcac cttcggacaa tcgtcgccgg cgcgtggcac
     4081 cgcgcaggtg atcgcccagg gcctgaccgc accaccggcc gacaaggtcg cctggcgcgt
     4141 gatcgagcag ccgatcccga aacggctcga cgcgcgacca tcaaaccgga tgcaggccag
     4201 caccggtttc ctgctcgccg acgaaacgcc gatcttcgtc gccgatcagc gaaccaagct
     4261 gcgctcccgc cttgctcctg gcgaggctca attcgttccg accggagcca accagacctg
     4321 ggccagcctc ggagaggacg ccgggaccgc ctattcgctg gaactggtgg atcgggaagt
     4381 cgcgaaggtc agcccggagg caaagatcct ctatcgcagc ggcagcttct cgatgaagcc
     4441 gggcgattac gacctggatc tgattcgcga cgacgtcaaa tccggcgagc gggcgaagat
     4501 cgaggatggc agcttcccgg tcctggtcct ggtcacgtcg ggacaggtcg aggtgacgtc
     4561 gaccaacgcg aaggaaccgg tccggctcaa ggagggccag gcaatcgccc tccgcggtga
     4621 gttgaccatc cgggcgcgcg gcaaggactc gacatatgtc gccgcgatca tgggcgatgc
     4681 cgtgtccgga ggcgaagccg tgccgaccgt ggagccgacc agcacgccga agccgaagaa
     4741 gaccagtacc cctgagccag aggcgaccaa gaagccgaag aagaccaaga ctccgaaggc
     4801 cgagccgacg gagactcccg gcgtcggcga cggtgcgtca gtccggattg ccgttcggct
     4861 ctgccgcgag ggcatgacct actttgctat caatccccag gcatgcgagc gcgccgacgg
     4921 tgactatcaa ctcacgctcg tggcgccgaa cggcaggcga ctgcacctca gcgacgccag
     4981 ccgggtcgaa ccgaacttcg tgcgttggag cgggctcaag cccggtgact acgagttggt
     5041 cgtcggcaag ctgcccgatg gctacaagag ctattcgctg gacggcttcc tctgctgcag
     5101 cacgaacgag gggtactcga tcaccgtccg aaagaatcag ctgatcgatg gaaccctcta
     5161 tctgttcacc gaggactggg gcgtaggcgc gccgccggcg ccgactccga tcccgccagc
     5221 tccgcaaccc ggcaatccgg ctcccggtgc cgattcggac ggcgatgggc tgtcggacga
     5281 gctggagacg gacgtcttcg gcaccagtcc attcctcgtc gactcggacg gagacacgat
     5341 tccggacggc gtcgaggcgt tcggcagcaa cgggtacctg accgcgcccg ccttgccgga
     5401 caccgacttc gacggcgtca tggacaacgt cgaaatccag aagggcaccg atccgctcga
     5461 cccgaatagc cgctagcgcc gaccggtcag catggtgcac gagcagcggt tcgaccagtt
     5521 tgcgcgcgcg ctggccggcg ttgccaatcg tcgctccgtc atggccggcc tcgtcgctgg
     5581 tatcggcgcg ctgctgtccg gtcgatcggc tcgcgccggc tgcgttgcac ccggcctgcc
     5641 ttgcaccaca gaggagggct gctgtctcgg ccactcgtgc accgaaggga catgtgtctg
     5701 cccgagcgga cagtgggacg cggtgggcaa ctgcattccg cttgccccgc cgtgtctcgc
     5761 accgggaacg ccgtgccaaa tcacggagag ctgctgctcg ggctattcat gcttcgaagg
     5821 gatctgtgac tgcccgagcg gcatgatcga ggcaaccggc ttctgcggat caaactccgc
     5881 gccctgcatg ggcgatagct tcccgtgtgg atcggacgcg ccatgctgtg acggtttcga
     5941 atgccaggaa ggctcgtgcg tgcaggtcgc cgcactggtc tgccgggcag tcggcgaggc
     6001 gtgccagacc aaacgtgact gctgtccgga gatggccgcg tcgtgcgtcg atggcgtttg
     6061 cgcctgccca caccggcgcg cgctcgaccc gcaagtcatg gtctgcaatg gcccctgcgc
     6121 catcccgggc gagcaatgcg acgagcagac gccctgctgc gacggcgcgg gaatgtgcat
     6181 tcgtggcgtc tgcacttgcg cgaaggacga gctcgtttgt ggcgcacaat gtgagccagc
     6241 cgaaggcaac ttccagcgca acaaccgcaa ttgcggcggg cgcggcctga tttgcgaggc
     6301 cgatcaagtc tgctgcgaag gactctgcat cgaccggacc gcgttgctcg acgatccgga
     6361 ccattgcggt tcgtgttgca ctgcctgtcg agccggccag atctgttgca acggccggtg
     6421 cgaggagccg ttcagtgatt ccggcgatcc ggagatctgc tgcgacactg gcagtatctg
     6481 cgtcggtgcg gacgggtcgg agatctgctg cgggccaggc gacgcctgca catcggatgg
     6541 ctgttgcccg caggatcgag tgtgtggaga agagtgctgc ggcgacggca ccttctgcta
     6601 ctgcggtgcg tgcgtcactg acctggcgac gtgcgagagc gatgccgact gctgcgccgg
     6661 atcatgcacc ggcggcttct gttgcccgag cgaatcgatt tgtcacgatt tcgccggcaa
     6721 tccggtttgc tgcggcggcc tcagcctctg cgttgaagat acgaacgttt gcgtcgcgcg
     6781 cgagtatggg agcctggatc ggccggtgcg ctgggttgcc tgatcaggcg aagctcggtg
     6841 cgaccgtctc gccgagcaag cgaatcgtct ccagatcgcg cgaatcctga atttgaatga
     6901 cgaagtactc gaagccgatc ccgatcagtc cctggaaata gtcgatgacc ttctgtgggg
     6961 agccgaagat ccgggttcga tgctgctctt cagtcaatcc gtccgggtag tagcgatcga
     7021 gcttcgcctt ggctgattct tccgtctccg caagcatcag ccaggacgtg aaatgactct
     7081 tgagcaaggt cgcgggatcg cggccctctt cgtcgcagag ctggtcgatc agctccagtt
     7141 tcttgcgcgt cgcctcgtcg ctggtcacgt ttcccgtgtt cttgctggcg ccgaagttgg
     7201 cgatgtcggc ccagcggacc acctgacgaa gcgtcttctc gcccgcaccg gcaaccagga
     7261 tcggcgggcg tggtcgctgc accggcggag aggtgatgtg ggcgttgagc acgtgccagc
     7321 tctcgccggt gaaggtgatt ggctccgggc cgaacatccc gtcgatgatc tggagcgcct
     7381 cttctagtcc tcgttggcgt tcaggaaccg ggcgaaacgg aacgccgagc tgggcaaact
     7441 ccggctcgtt ccagccgatg cccaggccga gcatgacccg cccgttgcta attcggtcga
     7501 gatcggccgc catccgggcc agcattgacg gctgccggtg gaagacgcaa ttgacaaccg
     7561 agccgagctg gacattggac gtgcgcgcgg ccaggttcgc caaatgcagc cagggctcga
     7621 tgtggtaacc gggatgatcg ccgatgaaga agccgtcgag tccgacctgc tccgcgagga
     7681 gcccggcttc gataacgctc tcggtcgggt tcgtatcgtc gggtaacgga aatgcctgaa
     7741 ccgcaaaccg gactgatttc cgacgctcag ctacccaggg atgtgccgtg cggaagtcga
     7801 ccatcgctcg cgtacctcct gccctggatc atcgcacgat tcggcattcg gggctgtatc
     7861 gcaccatctg gttggcgaaa atcgtggcac catttcgcgg gcgcattgcc ggttgcgcga
     7921 acggggtcgg aagggaagcg agtgcagatc aaggcgttga tattcgatat ggacggcttg
     7981 ctggtcgata ccgaaccgct tgcctaccag gcgatggacg cattcctcgc caagcatgag
     8041 gtagagcggc gccaggatat ccacgaccag atgctcggcc gccggctgcc cgaggcgatc
     8101 gcgatcgtca aggaggggta cggtctgacc catccgctcg acgacctaat cgccgactac
     8161 accattatgc gccgcgacat cctggtcgga aacgtgaagc cgatgccggg cgcgatggag
     8221 gtgatccggt tcgggcacga tgccgggctg aagaccgggc tggcgtcctc cggcctgcgc
     8281 gatcaggtca cgctctcgct gaatgaggcc gggatgcgtg gcatgttcga ggtcgaagtg
     8341 acgggcgacg acgtgacgcg aggcaaacct gcgccggagc tgttcctgaa ggccgcggaa
     8401 ggactcggca tcgatcccgt aaattgcgtc gtgttcgagg atgcgccagc tggcgttgcc
     8461 gcggccgtca acgccggcat gcgtgccgtg gcggtgccga atcagcacag tcgcgagatg
     8521 gaattcgcgg tcaagccgga cgctgtcctg acctcgctgc acgatgcgat cccgtggctc
     8581 aagcgccacg gagttggccg gaagtagggg cagtggagcg ccacgtctac gatctggcga
     8641 ttgtcggggc cggaattgcc ggcctggccg cggcgcgtcg cgcgatcgat ctcggtttga
     8701 gcgtcgtggt gctcgaggcc accgaccaga tcggcggacg gattcggacc gttagccggc
     8761 ctgacggctc gatctgggac gcaggcgcgc attggctgct ccagccagcc gtcaatccgc
     8821 tcgtgcgtga ggcggagcgg ctgggggtgc ggttccggcg gcaatggcgc gcgcacgacg
     8881 gctattggct ggacggtcaa tggctgagcg aactcgaaag tgacgcagtc tggctcgaca
     8941 tcgacgacgc cgaggacttg gcgagggagc tgtcggctgc cgacgaggat gtcgcattcg
     9001 ccgaactgat cgatcctgaa tcgggcgccc tggcaatgat cgagacgatc ctgactcagc
     9061 aatatggcga gcatccgggt cgaatctcgg caatcgaccg ctcccgctac ggcgtgttcg
     9121 aaggcgactg gccggtgatc ggcgggttcg gccggttcgt cgagcaactc tttgccgatg
     9181 tgccggttcg gcctgggtcg ccggtatccg tgatcgactg gggcgatgaa cctgttcgcc
     9241 ttgatgtctt cggagatttg gtcgaggcgc gaaaggtgtt ggtcaccgtg tcgaccggcg
     9301 ttcttctatc gggtcaggtg ctgttcgtgc cggagctgcc tgccgacaag gtggccgcaa
     9361 tatcaagtct cccgatggcg gcacaggaca aggtggcgtt ccgaatcgat cccactttgc
     9421 tcgactgcac ggccgactcg attcagtaca cgcgcgccgg ggattcgatc gtcattttca
     9481 acgtcctccc cggcgggcag ccgttagtgg tggcgtacat ttctggctat ctctgccgcg
     9541 atcccgagcg gcaggggaag gacgcgctga ttggtgctgt catcgagcaa ttcgaagaag
     9601 tctacgggcc agaagtagcg gcaacagtct cggatccgga agcggcgatt tgggggacgc
     9661 acccatacgt aggcggcgcg tgggcgtacc ccgaacccgg cgcgctgacg gcccgagccg
     9721 ttttgaccga accggtcgac ggtcggctct ggttcgcagg cgaggcgacg agccagaatg
     9781 ccgccggcac ggttcatggg gcatacgaga gcggtctcaa tgtcgctgag ctgatcgctg
     9841 agtcgctggg actcaaggtc gaaacgccgc ttggcgggga taatcgcctg atctgattga
     9901 tggcggattt cacggaaggt aatgcaaacg tatgagctct cagcgagcag tagatctcgt
     9961 tatcatcggc gcgggtgcgg ctggactcgg agcagcgcgc cgtgcgggcg agctcgggct
    10021 gtcgtacaac gtctgcgaag cgatggaccg gatcggcggt cgcgcccaca cggattcgaa
    10081 gacattcggt acgccgtggg atcgtggctg ccactggctg cactcgggca gcgtcaaccc
    10141 gttcgcgaaa cttgcggatc agtacggctt ccactacgag tcgaccccgc cggtgcggcg
    10201 cagctttgat ggctcccgct ggatttccgg cgacgagcag gctgagctcg agaagcacgt
    10261 ctacggagac ctctggggcg cgatcgagcg cgccgggaag gccggcaagg acatctccgc
    10321 cgccgaggtc gtcgatatgc acgacccgtg gatagcgatc ttgcggaccg cgttggccgg
    10381 cgagtggagc gtcgatattc cggacgtttc caccggcgac gatgttgcct atcgcgatac
    10441 gcacgagaac tggccggtcc gcgaaggata cggcgcactc gtcgcgcgcc acgccgaggg
    10501 tatcccggtc gagctgagca cgccggtgac gaagatcacc tgggatggtc ccggggtcga
    10561 ggtcgaaacc ggtgcgggaa cgatctcggc gcgggcagtc gtgatcaccg tcagcacgcg
    10621 catcatccag gacgacgtaa tccagttttc accccggctg ccggattgga aacgcgaagc
    10681 gtatggcgca attacgcttg gaaacgccaa caaggtctcg ttcaagatcg acaagaacct
    10741 gctcggcgat gcccacaata ctgcctggat ccgcgccacg ccggatcagg ggatgtggtt
    10801 ccagatgcgg gccttcgacc gggacatggc gaacggctac atcgccggcg cgctcggcga
    10861 ggcagccgag gccgagggag aggcggcact gatcgcgtgc ggccgcgagg cgctcaagtc
    10921 ggcctatggc agcgacatcg tcaaggcaat ccaggtcgag gcgtgcacga catggcagag
    10981 cgagccatgg atccgcgggg catacggagc ggcccaaccc ggcaaggcgc acttgcgcaa
    11041 ggatctggcg acgccaatcg acgaccggct ttacttcgcg ggagaatcga cctcactcga
    11101 cttcttctcg acctgccacg gagcgcacct gacgggcatc gccgctgttg agtcggcagc
    11161 caaggcggta ggtcgctgag cagcgtggag attatcctcg cgaccacgaa ccgcgcaaag
    11221 gtccgggcgc tggcggagag agtgggggcc gggatagtcg ttgccccgct gccgagcgag
    11281 acaggtgagc gacatatccc gctgaagctt gaagatggcg tggacgcgac cgcaattgcg
    11341 cggacaaagg ccagctggta ctcacgacat atcccgaaaa tgttcacagc agcgagcgac
    11401 ggcggcatcc tcattcccgc ccttggacgt gactggaatc cggccagaac tgatcgcatt
    11461 tcaggtgccg atccccggga agccacccgg gtggaggcac tcctggcgcg tgctcgacat
    11521 ctcgtaggtc ctgaccgaca aatcgggtgg accgaggctc ttgccgtcgc ccgggatggc
    11581 gagattctgg cggaatgggt ggcgcacagt ccaccgggcg agttggctgt cgatctgcca
    11641 gatgatttcg accctgaggc cggcttctgg gtgccgtatg tctggcgatg ccccgaatac
    11701 ggtgggcggc gcctctcgga actgaccgaa catgaacgtc tggcccgccg tgatcactgg
    11761 gcaattcttg gcgagaacct gaccgagtgg gcgctcgacc aaaccggatc aacgtcgtag
    11821 ccccgcgcta cgtttgcctg gaggtatctc ggcgtgcctc gcaccatgcg gcaacgggga
    11881 gggcgtactc ggcaatgcct ttgtaggcga gcactgccgg cgatgctgac cggagtatcg
    11941 tggccaggcg ggagatcagg tccggtcgat tcccggccaa ctccggcaac gacgtcgagt
    12001 agatccggac cgtgaaaagc gcacggccca gatcgggcat cggataagtc gtctgccgct
    12061 caatccggac cgagaccttg cttgccagca ggtccggatc ggcgatccag gcggggtccc
    12121 atggtttcgg cagatccgag tggttgtcga gcgatggtaa cgtcgtcagc gagagaccga
    12181 atcggatgta tggccccttg gtcacgatcg ccttcatgac gttgtcggcc gaggccacga
    12241 ggcgagcgct atccgcgacc ggctcgtgaa tcgtcccgaa cgattgctgg tacttctcgc
    12301 gcggattcca ggcgctcggc aggaggacat gcagcgattc tgccctatgt cggccgtcgg
    12361 gcaggagccg catgatcacg atgtcctcct ggcaggcaag ggccagggca tctccgaagc
    12421 gatcgagccc ggactgcgca tcgatccatc gcgcaaccgc ccgcccgatc tccgaagcat
    12481 cggatcggac cgtgacgccg acatgctgcc ggtcaccggc gtcgatcgtc agaccaggca
    12541 accgcatgtg gatggtctcg ccatcgatct gcacgtattc cggatgctcg gccgcaagga
    12601 ttcggaatat gcgccagtag acctccgcca gcgcatcgga ttcgtccggg tcgaggcacc
    12661 ggtacctggc aggatcgcgc gcgagtgccg ccaacttctc cgtcacgaac aggtcgaact
    12721 ggttgtccag ttcgaaatga cgagtctcac gccgcccgtt gtaggtgccg ggcaacggaa
    12781 aaaggtccgc gcgaacaatg aagtcgtcgc ggaccaggaa cggctgatat ggagcagata
    12841 gcggtggcaa tacccgcacc gggcgtcaga ccatcgtgtt ggtcagcttg cccaactttt
    12901 cgacttcgac ttcgacgaca tcacccttct ttagccacgg ctgattgggc atgcccatcg
    12961 cgacgccctc cggcgtgccg gtcgtgatca gatcgcctgc ctcgagcgtg aggtacttgc
    13021 tgcagtagct gacgagctgc gcgacggtga agaccatgtc ggacgtgttc gagtcctgcc
    13081 gggtctcgcc gttgactttg caggtgaggc gcagggtttg cggatcgccg atctcgtcgg
    13141 cagtcactag gtacggaccg atcgggaaga acttgtcgga ggtcttgccg aggagccact
    13201 ggctagtccg catctgcaga tcgcgcacac tcaagtcatt ggcggtgcag taaccggcaa
    13261 cgtacgagag ggcatcagcc tcggagacgt tgcggacggt cttgccgatc acgacgcaga
    13321 gctcaacttc gtaatcgaac tgcgacgcga catcgtcgat cagcgggaca tcctcgttgt
    13381 gcccggcgag cgagttgttg aacttggaga acagcaccgg attggtcggg atcggcatgt
    13441 tcgattcggc ggcgtgcttg gcgtagttca gtccgacgca gatgatcttg ccgggatccg
    13501 caacgcacgg cccgagcgtc agcttcgact cgtcatgcag ccacgcttcg ctgccctcga
    13561 tcttgtcaac gagcgactgc agcttgtcga ggccatcggc cagtcccgcg acagtcgtcg
    13621 gcgcgtcgat tccgagagca agcccggccg aggcaacatc gatcacgccc ttatcggtcg
    13681 caattccgga tttgagctcg ccgtcagcct tgtacgtcag caacttcatg agatgcgcgt
    13741 gtcctctcca tgaacatcga atgttggcac cgactggtag ggtaccaaag cggcccgcac
    13801 tatttgcggt gctcgtcccg gaacgagatc agacggcggc atccctcggc cacgtcctca
    13861 tccgaggagg ccagcgagat gcgcacgaag ccgtccgcca gcaccttgcc gaaggtatcg
    13921 cccggcgcgg tcgccacgcg cttttccttg agcaacgcca tcgccagatc ccgaccgttc
    13981 atcccggtct cgcggaggtc gaccagagca tagaaggcgc ccttcggtcg aaccggaagc
    14041 agaccggccg gctcgaggat cccagccgcc aaatcccggc gcttgcgata ggcgttgcac
    14101 atcacctcaa ccggctgttg ggtgccggtc agcgcggcga tcccggcctg ctgcgaaacc
    14161 gaggacgggc aagaaatcag cggctcgagc atcttgttga cagcctggag gagcgagacg
    14221 tcggcaatcg cccagccgag tcgccagccg gtcatcgcgt acgtcttgga gaagcccgag
    14281 acggtaatca cccgtcctgc gtcgtcgaat gccgctgctg gcacgtgctc gccgtcgaag
    14341 accaactcct cgtaaatctc gtccgacagg acgaagagat catgtttcgc cgccagctcg
    14401 acgactgcct tgaccgtttc ctccggccag accgcaccgg tcgggttgcc ggggttgcag
    14461 gtgataatca tcttcgtctt gggcgtgatc agcttctcga gcgcctcggg atcgggcagg
    14521 tagccgttct cgaccgacat cgggtacagg accgagacgc caccgaccag gttcaggatc
    14581 tggcgatagt tcggccagcc cggatccggg accagcacct catcgccgcg atctatgagg
    14641 gcgaagatcg ttgagttgat cgcgttcacg ccgccatggc ttgccatcac catgttcggc
    14701 gtgacctcct tgccccactt ggcggagtat cgatcggcga tcgcctggcg aaggggcagg
    14761 attccgccgc tctgggtata gcgcgtcgcg cctgcggcgg tcgcctcgaa tgcctgctcg
    14821 atgatgtggg ccggcgtcgt gaaactcggc tcgccgactt cgaggtggat gatctcgccg
    14881 cccatttcgc gcgcggcatc catcaggtcg cgaatgccgg accgctcgat cgagcctgcc
    14941 aggtgtgaaa tcgaacgcac gtgtgcctcc aggaatgatc cacggttacc ggagggattg
    15001 tatgagacag cacccaccga gtcagttccc gtgcttgatg gtgtaaacat ttccgtggcg
    15061 caacgtcttg ataagtgcat gggtaaatcg gcgtatcggg gcgcaggggc atggtggaat
    15121 accaaccaca cgccatcgcg gactaccggc ccgtcctctc cgcagaatct gacgagattc
    15181 tggcagcgcg cgctgctacg gatgcggccg cattcgccga gatctacaac cgatacgtcg
    15241 cccgaataga cgggtactgc cgccagcgcc tgcacaacgc ggacctggcg gaggacgcaa
    15301 cgcaccagat cttcctgcga gcactcgaga gcctgcggaa gcatccggtc gacaacgtgg
    15361 cagcctggct gttcaccatc gcgcacaacg aatgtattga tcggtaccgc cggcaccgcg
    15421 cgcacgtgcc gatagagatg gcggctgaaa tggaaggttt cgaccggtcg cccgaggatc
    15481 aggctgttgc tcgaagcatg gccaacgagc ttcgggatct cctcccgttg cttagtgagg
    15541 atcagcggcg ggtgctcgag cttcgcctcg ctggactctc gagcggggag atcgaaatcg
    15601 tccttggcaa gagtcgatcc tgggtcggcg ttattcagca ccgggcagtt cttcggttgc
    15661 ggacattgat ggtcgttcag gctgagccag aggagcgata gatgcgctgg cttcgaggca
    15721 tgaaccacca gaatgagagt gacgcgctga acgaattctg ggacgaagtc gtccagcgga
    15781 gccccgcgac gccgctgacc cgcgacccgg tccccactga gctcggcgca atcgttagac
    15841 aacttcatgc ggagcaggac gcgcggcagc agcgaccggc ctacgcggat cggcagctcg
    15901 ct
//
LOCUS       DATAOZ010000572         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8640986, whole genome shotgun sequence.
ACCESSION   DATAOZ010000572 DATAOZ010000000
VERSION     DATAOZ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8640986"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VHR64_14450"
     CDS             <1..1279
                     /locus_tag="VHR64_14450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX3304084.1"
                     /translation="VQQSYATPEAQTTPVAASSSYGDTRRSIATFIYTGDGSIRESKP
                     SGYTDDPDSPPKLPAFGSKEFQALVGKLVTLPSEDGSIKYRVLIQPGRIPGDMVVVAE
                     SLSEVDSSVRSLLIRLIAIGLAGLLIAAAASWWVIQRQFRPVDKMIDTAAAIAGGDLA
                     SRVPEGDPRTELGKLGGALNEMLGQIEESTRQREANEQRLRRFVADAAHELRTPLTSV
                     RGYAELYRQGAYQDDVALNNAMARIESEGGRMARLVDDLLLLARLDQQRGLEQRPLDL
                     VSLAGEAAADFETVSNDHQLNWRPDGEILVRGDRIRLRQVIDNLLNNARMHTPAGTSI
                     DMSVTRNGTAAELVITDNGPGIPADDQHHIFERFWRADRSRTRNTGGTGLGLAIVQSL
                     VQAHGGTVLLESNPGQGASFTIHLPLLKTQPEP"
     gene            complement(1581..>1911)
                     /locus_tag="VHR64_14455"
     CDS             complement(1581..>1911)
                     /locus_tag="VHR64_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304085.1"
                     /translation="FLEWKTIYDEGGIFGTTDGNACDLWFEGEANGARNDHDGAGLNH
                     LAVITESQEDVDTAVAYLIGKDVAMLFGTPCNRPEHAESENHLYYSAMFESPDRILIE
                     IVYTGPK"
BASE COUNT          342 a          684 c          563 g          322 t
ORIGIN      
        1 agtccagcag tcgtacgcga ctcccgaagc tcaaacgaca ccggttgccg cgtccagcag
       61 ctatggcgat acccgtcgca gcatcgccac cttcatctat accggcgacg gctcgatccg
      121 cgagagcaag ccctccgggt ataccgacga tcccgactcc ccgccgaagc tgcccgcctt
      181 cggcagcaag gagttccaag cgctggtcgg caagctcgtg accctcccgt ccgaggacgg
      241 ctcgatcaaa taccgcgtcc tgatccagcc aggccgcatc ccgggcgaca tggtggtcgt
      301 cgccgaatcg ctttcggagg tcgactcctc cgtccgcagc ctgctcatcc gcctgatcgc
      361 gatcggcttg gccggcttgt tgatcgctgc cgccgcgagt tggtgggtca tccagcgcca
      421 gttccggccg gtcgacaaga tgatcgacac cgcggccgcg atcgccggcg gggatctcgc
      481 gagccgcgtg cccgaaggcg atccccgcac cgagctcggc aagctcggcg gcgcgctcaa
      541 tgagatgctt gggcagatcg aggagagcac ccgccagcgg gaagccaacg agcaacgcct
      601 gcgccggttc gtggccgacg ccgcgcacga gcttcgcacg cctctgacct ccgtccgggg
      661 ctacgccgag ctctaccggc agggcgccta ccaggatgat gtcgcgctca acaacgccat
      721 ggcccggatc gaatccgagg gcggcaggat ggccagactg gtcgacgatc tcctgctgct
      781 cgcccgactg gatcagcagc gcggtctcga gcagcgtccg ctcgacctcg tctccctggc
      841 cggcgaagca gccgccgact tcgagaccgt cagtaatgac caccagctca actggcggcc
      901 ggacggtgag atcctggtcc ggggcgaccg catccgcctc cgccaggtca tcgacaacct
      961 gctcaacaac gcccgcatgc acaccccggc cggcaccagc attgatatgt cagtcacgcg
     1021 gaacggaacc gccgccgagt tggtcatcac cgacaatggt cccggcatcc cggccgacga
     1081 tcagcaccac atcttcgagc gcttctggcg agccgatcgc tcccgcaccc gcaacaccgg
     1141 cggcaccggc ctcggcctcg ccatcgtcca gtccctcgtc caggcccacg gcggcaccgt
     1201 cctcctcgaa agcaaccccg gtcagggcgc cagcttcacc atccacctgc ccctcctcaa
     1261 gacccaaccc gaaccatagc gccggccccc cgtggccggc tttgtgtccg tagtggcgtg
     1321 gctccgtaca cgccttgact cgcctgtggg tcagcttcgt ggtgctggtg agcggtcggt
     1381 cggcggcgcc cggggataaa tcccggggct gaatccaata agccagctga agcaggctga
     1441 acggcacgcg tcacgtagcg cggtgcatac cctctccggc tctccctgtc agggagagag
     1501 catccccgcg atgacgcgaa gcggcatgac ggcctcacaa atagcaagcc cggtgaatgg
     1561 ggctgctcga cctgttaccc ctatttcggg cctgtataga cgatctcgat caggatccgg
     1621 tcgggcgact cgaacatggc tgagtagtag aggtggtttt cgctttcggc gtgttccggt
     1681 cgattgcagg gcgtcccgaa aagcatcgca acgtccttac caatcaaata ggcaactgcg
     1741 gtatcgacat cctcctggct ctcggtgatc actgccaggt ggttcagccc cgcgccatca
     1801 tggtcattcc gggcgccatt ggcctcgcct tcgaaccaga gatcgcacgc attgccatcc
     1861 gtggttccga aaatgccacc ctcgtcgtag atggtcttcc actcaaggaa t
//
LOCUS       DATAOZ010000573         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6271401, whole genome shotgun sequence.
ACCESSION   DATAOZ010000573 DATAOZ010000000
VERSION     DATAOZ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6271401"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VHR64_14460"
     CDS             <1..321
                     /locus_tag="VHR64_14460"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX3304086.1"
                     /translation="EYEDLKEDLVMTDRRIQELEYLLATAEPIEPSKDGSVHLGSVVT
                     IVDSDGEEATWTLVDPAEADTRAGSISTDSPVGRALMGRHKGDSATVETPGGPIVYTV
                     VNVA"
     gene            413..>1721
                     /gene="lysS"
                     /locus_tag="VHR64_14465"
     CDS             413..>1721
                     /gene="lysS"
                     /locus_tag="VHR64_14465"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX3304087.1"
                     /translation="MELSDLQEIRRQKAASLRDRGLNPYSPRSERTHTTADAISRFEA
                     IESAADNAAAEDQDPITVAGRVISFRDMGKTLFSHIRDGHGDLQLYVRLNDVGEQAHA
                     DFSTLVDLGDFVEATGTLFRTRTGEVSLKVREFSILTKSLNAPPEKRKGLRDREIRYR
                     QRYADLIANADVRETFRFRSALVAAIREFLQAEDFIDVETPILQPLYGGAAARPFTTH
                     YNALGNEFYLRIADELYLKRLIVGGFERVFEISKDFRNEGMDRTHSPEFTMLEWYEAY
                     SDYLIVLERLERLFQHIATTVLGGKTFVNNGHEIDLSTPWPRLTLREAIRQFSGVDYV
                     EHPDQSSLLAAARAAGADIEDGTVWPRIVDELHKQFVRPSLIQPTVLMDYPVELSPLA
                     KRKQDDPTHVERFQLIMAATEITNAFTELNDPLDQLQRFLEQQR"
BASE COUNT          335 a          569 c          515 g          302 t
ORIGIN      
        1 gagtacgagg acctcaagga ggacctcgtg atgactgatc ggcgcatcca ggagctcgaa
       61 taccttctgg caactgccga gccgatcgag ccgagcaagg acggatcggt ccacctcggt
      121 tcggtcgtga cgatcgtcga ctcggacggc gaggaggcaa cctggaccct ggtcgatccg
      181 gccgaggccg atacgcgcgc cggctcgatc tcgaccgatt caccggtcgg ccgggcgctg
      241 atgggtcgcc acaagggtga ttcggccacg gtcgaaaccc cgggaggccc gatcgtctac
      301 actgtggtca acgtcgccta gccacgcaac cagcgatcga aaccgatacc gccgggtgca
      361 aacgcccggc ggttcttttt tgcctcaact cacaagccct ggagcgatcc ccatggagct
      421 gtctgacctt caggaaatcc ggcgtcaaaa ggcggcgtcg cttcgcgacc gaggcctcaa
      481 tccctattcg ccgcgctcgg agcgcaccca caccacagcg gacgcaatct cccggttcga
      541 agcaatcgaa tcggccgcgg ataacgcggc tgccgaggat caggacccca tcacggtcgc
      601 gggtcgagtc atctcattcc gggatatggg caagacgctt ttcagccaca tcagggatgg
      661 ccacggcgat ctccagcttt acgtccgcct caatgatgtc ggggagcaag cccacgccga
      721 tttcagcacg ctcgtcgacc tcggcgactt cgtcgaagcg accggcaccc tcttccgtac
      781 ccgaaccggt gaggtctcgc tcaaggtgcg ggagttctcg atcctgacca aatcgctgaa
      841 tgcgccgccc gagaagcgca aggggcttcg cgaccgcgag atccggtacc ggcagcgcta
      901 cgccgacctg atcgccaatg ccgacgtacg ggagaccttc cggttccgct ccgcgctggt
      961 cgcggcgatc cgggagttcc tgcaggcgga ggatttcatc gatgttgaga cgccaatcct
     1021 gcagccgctg tacggcggcg cggcggcccg gcccttcacg acgcactaca acgcgctcgg
     1081 caacgagttc tacctgcgca tcgccgatga gctttacctg aagcggctga tcgtcggcgg
     1141 attcgagcgc gtcttcgaga tcagcaagga ctttcgcaat gagggtatgg accggacgca
     1201 ctcgcccgag ttcacaatgc tcgagtggta tgaggcatat tccgattacc tgatcgtcct
     1261 cgagcggctc gagcggctct tccagcatat cgcgaccacc gtcctcggcg gcaaaacctt
     1321 cgtgaacaac gggcacgaga tcgacctctc gacgccatgg cctcgcctga ccttgcgcga
     1381 ggcaatccgc caattctcgg gcgtcgacta tgtcgagcat ccggaccagt caagccttct
     1441 tgccgcggct cgtgcggccg gcgccgatat cgaggacggc acagtttggc cgcgcatcgt
     1501 cgatgagctc cacaagcaat tcgtccgccc gagtctgatc cagccgacgg tgttgatgga
     1561 ttacccggtc gagctctcgc cgctggccaa gcgcaagcag gacgatccga cgcatgtcga
     1621 gcggttccag ctgatcatgg cagcgaccga gattacgaac gcattcaccg agctgaacga
     1681 tccgctggat caactccagc gtttcctcga gcaacagcgc g
//
LOCUS       DATAOZ010000574         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4429726, whole genome shotgun sequence.
ACCESSION   DATAOZ010000574 DATAOZ010000000
VERSION     DATAOZ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4429726"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="VHR64_14470"
     CDS             <1..1770
                     /locus_tag="VHR64_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX3304088.1"
                     /translation="ESLGSATVICTDKTGTLTSGAMTCTVIRFADRQVEISGAGYAPE
                     GQFTTGGAAVNPLTDTQLETALRIAALASRGDVVKDEAGWKPQGDPTEVALIIAARKG
                     GLDRTRLQLHWPEVAEIPFSSERMLTATYHRTPQGIVAYVKGAPARILELATSVLTVD
                     GVRAIRNGERDELLEQNRTLARQGMRVIALATMRADGQPVGELAGLVWIGLAGLMDPP
                     ADGVAETIVTLRRAGIRTIMLTGDQRLTALAVARQLHVITPGEHAVDGRELDQLSDDA
                     LLKLVATTSVFSRVSPRAKLRIVDAYQKQGEIVAMLGDGVNDAAALRKADVGVAMGLR
                     GTDLAKEAADVILEDDRFTTIAAAVEEGRVIFDNIRKFVFYLFSCNLAEILVMFGAGV
                     AGLPAPLRPIQILWLNLLTDTLPALALALEPGERGIMRQPPRDPRRGIVSPRMLRAIA
                     GYGLFIAACALGVFAWALAEDGNIGRAATMTFMTLALAQILHLGNARSEGSVTTLRRI
                     VANWFALGAVALAILLQLISLTGFGRVMGWERLGWEDWLTIGIIASLPAVAGQTIKTV
                     RAAINTPPARQQAVRSPEPGSQG"
     gene            complement(1709..>3603)
                     /gene="ppsA"
                     /locus_tag="VHR64_14475"
     CDS             complement(1709..>3603)
                     /gene="ppsA"
                     /locus_tag="VHR64_14475"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX3304089.1"
                     /translation="AIVYRAQHKIDHRSVALSVGVQKMVRSDLDSAGVIFTLDTESGF
                     RDVVLITGSWGLGETVVQGRVRPDEFWVHKPTLRQGHRSIVRREISEKSVKLVYAEES
                     GKTVREVRVPRRSRRLPVLSDDDVLTLAQWSLIIEDHYSERAGRPTPMDIEWARDGRN
                     GELFILQARPETVHSQAQRATLEMFHRKGAGEVLLTGQAVGSKVATGKVRVIHSVDSL
                     ADFQAGEVLVAPMTDPDWEPVLKGAAAVVTDEGGRTCHAAIVARELGIPCVVGALRGT
                     QILKDGQEVTISCAEGEEGKVYAGRVPFDREEIDPTTLPTPAVPLMLNLANPERAFYL
                     AQLPSDGVGLVRIEFVVSNWIGIHPMALLHPERITDDAAGIEILKRIGDTRRPAEFFV
                     SRLAMGVAQIAAAFYPRPVIVRFSDFKTNEYAGLLGGKDFEPVESNPMIGFRGASRYY
                     DERYREAFALECAAIWRVREEMGLTNVKVMIPFCRTLEEGRRVLREMAQNGLIRGQNG
                     LEVWVMCEIPNNVILASDFSDLFDGFSIGSNDLTQLTLGIDRDSELLRDLFDERDPGV
                     ERLIQMVIAAAHQKGRRVGICGQAPSDYPEFAEYLVRIGIDSISLNPDSLARVTARLA
                     AGPEEC"
BASE COUNT          679 a         1183 c         1093 g          648 t
ORIGIN      
        1 gaaagtctcg gctcggccac agtgatctgc accgacaaga ccggcactct gacctctgga
       61 gcgatgacct gcaccgtcat ccgattcgcc gatcgccagg tcgagatctc gggcgccggg
      121 tacgccccgg agggtcaatt cacaactggc ggtgcggcgg tgaatcccct caccgatacc
      181 cagctcgaaa ctgcgcttcg gatcgcggcc ctggccagcc gtggcgacgt cgtcaaggac
      241 gaggccgggt ggaagccgca aggcgatccg accgaggtcg cattaatcat tgctgcgcgg
      301 aaaggcggtc tcgatcgaac acggctgcaa cttcactggc cggaagttgc cgagattccg
      361 ttctccagcg aacgaatgct gacggctaca tatcaccgga ccccgcaggg aattgttgcc
      421 tatgtaaaag gcgcgccggc aagaattctg gagctcgcga ccagcgtgct taccgttgac
      481 ggggttcgag ccatccgcaa cggtgagcgc gacgaactgc tggagcagaa ccgcaccctc
      541 gcgcgacagg ggatgcgggt gatcgcctta gcgaccatgc gggccgacgg acagccggtc
      601 ggcgagcttg cgggactggt atggataggt ctggcaggat tgatggatcc accggccgac
      661 ggtgtggccg aaacaatcgt caccctccgg cgggccggta tccgcacgat catgcttacg
      721 ggcgaccagc gcctcaccgc gctcgccgtg gcccgccagc tccacgtcat aactccgggc
      781 gagcacgcgg tcgatgggcg cgagctggac cagctctccg acgacgcgct actgaagctg
      841 gtggcgacga cttcggtctt cagccgggtc agtcctcgag ccaagctccg gatcgttgat
      901 gcctatcaaa agcagggcga gatcgtcgcg atgctgggag atggagtgaa cgacgccgca
      961 gcgctccgca aggccgatgt tggcgtggcc atgggcctcc ggggaaccga cctggcaaag
     1021 gaagcggccg acgtgatcct ggaggacgac cgcttcacca ccattgccgc tgcggtcgag
     1081 gaaggccggg tgatcttcga caacatccgc aaattcgtct tctacctctt cagctgcaat
     1141 ctcgccgaaa tcctggtcat gttcggggcg ggggtggcgg gactgccggc gccactccgc
     1201 ccgattcaga ttctctggct caacctcctg actgacacgc tgccggccct ggcgctggcg
     1261 ttggaacctg gagagcgcgg tatcatgcgc cagccgccgc gcgacccaag aagggggatc
     1321 gtatcgccgc ggatgctgcg tgccatcgcg gggtatggcc tcttcattgc cgcatgcgcg
     1381 ttgggcgtat tcgcctgggc actagcagag gatgggaaca taggccgagc cgccaccatg
     1441 acgttcatga cgcttgcgct ggctcagatt ctccacttgg gaaatgcccg aagcgaagga
     1501 tcagtcacca ccctccgccg gatcgttgcc aactggtttg ccctcggagc tgtagcgctg
     1561 gccatcctcc tccaattgat ctccctcacc ggatttggcc gggtcatggg ctgggaacgg
     1621 ctgggctggg aggattggct gacgatcggg ataatcgcca gcctcccggc cgtggcgggt
     1681 cagacgatca agacagtgag agcggcgatc aacactcctc cggcccggca gcaagccgtg
     1741 cggtcacccg agccagggag tcagggttaa gcgaaatgga gtcgatcccg attcgcacca
     1801 gatattcggc aaactcaggg tagtcactcg gtgcctggcc gcaaatcccg acccgccggc
     1861 ctttttgatg ggccgcagca atcaccatct ggatcagccg ctccacgccc gggtcccgct
     1921 catcaaacaa atcgcggagc agctcggaat cacgatcgat acccagcgtg agctgggtaa
     1981 gatcgttgga gccgatggag aagccgtcga acaggtcact gaagtccgat gcaaggatca
     2041 cgttgttggg gatttcacac atgacccaca cctcgagccc gttctgaccc cggatcaagc
     2101 cattctgggc catctcccgg agcacccgac ggccttcctc gagggtccgg cagaatggga
     2161 tcatgacctt gacgttggtc agtcccatct cttcgcgaac ccgccagatc gccgcacact
     2221 ccagcgcaaa cgcttcgcgg tatcgctcgt cgtagtagcg ggatgcgccg cggaaaccga
     2281 tcatcgggtt cgactccacc ggctcgaagt cctttccacc gagcaacccg gcatattcgt
     2341 tggtcttgaa atcggagaac cgcacgatca ccggacgggg atagaacgcg gcggcgatct
     2401 gggccacgcc catggccagc cggctgacga agaactcggc gggccggcga gtgtctccta
     2461 tccgcttcag gatctcgatt cctgcagcat cgtcggtgat gcgctcgggg tgaagcagcg
     2521 ccatcgggtg aattccgatc cagttcgata ccacgaactc gatccgcacc aatcccacac
     2581 catcgctcgg cagttgcgcc agatagaagg cacgctccgg attggccagg ttcagcatca
     2641 acggcacggc cggcgtgggc aacgtcgtgg gatcgatctc ctcccggtcg aacggcaccc
     2701 gtccagcata cactttgccc tcttctccct cggcgcagga gatggtgacc tcctgcccat
     2761 ccttgaggat ttgcgtgcct ctcagggcgc ccacgacaca agggattccg agctcccggg
     2821 cgacgatcgc cgcatgacag gtccggccgc cctcatcagt gaccacagcg gcagctcctt
     2881 tgagcaccgg ctcccaatcc ggatcggtca tgggtgccac cagcacctct cccgcctgaa
     2941 agtccgccag cgagtcaacg gaatgaatca cccggacctt tcccgtcgcc accttgctgc
     3001 cgaccgcctg accggtgagc aggacctctc cggcgccctt gcggtggaac atctccaggg
     3061 tggcccgctg agcttgggag tgcaccgtct ccggtctggc ttgcaggatg aacagttctc
     3121 cgttgcgacc atctcgggcc cactcgatgt ccattggcgt gggacgtccc gcccgctcgg
     3181 agtagtggtc ctcgatgatc agcgaccatt gagccagggt caggacgtca tcgtcgctca
     3241 ggaccggtaa gcgccgcgaa cgtcgaggta ccctcacctc acgaacggtc tttccgcttt
     3301 cctcggcgta caccagcttg actgacttct cggagatttc gcgccgtacg atcgatcggt
     3361 gcccctgccg gagcgtcggc ttgtgcaccc agaactcgtc aggccgcacc cgtccctgca
     3421 ccacggtctc ccccaggccc cacgatccgg tgatgagaac cacatcccgg aagccgctct
     3481 ccgtatccag cgtgaagatc acccccgcag aatccagatc gctccggacc atcttctgta
     3541 ctccgacgga gagagccacg cttcgatggt cgatcttatg ttgggctcgg tagacgatgg
     3601 ccc
//
LOCUS       DATAOZ010000575         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7592515, whole genome shotgun sequence.
ACCESSION   DATAOZ010000575 DATAOZ010000000
VERSION     DATAOZ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7592515"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VHR64_14480"
     CDS             complement(<1..570)
                     /locus_tag="VHR64_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3304090.1"
                     /translation="MSDALFDRMLQAARRGSLSRRQVLERGLKLGMSTAAITTLMAAA
                     PEVGASPRPASLRSLSRSQGQATGTFTIIGDESSPDLDPHSAYSNSAARILVGTHETL
                     IMLKGESTTEFDPVLAESWEASADNSTFTFKIPAGVTFQDGDPCTAESVRASFQRFLE
                     MGMGPVNVISRFMPDASMMEVVDDQTITFN"
     gene            complement(645..1610)
                     /locus_tag="VHR64_14485"
     CDS             complement(645..1610)
                     /locus_tag="VHR64_14485"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX3304091.1"
                     /translation="MARHQILLDTDVGIDDALMIVAIAAEPDAEIVAIGSSHGNCPAA
                     QAARNAIRVLDVLGLDEVPVAVGLESPLAVPVSAPHVHGSDGLGDACVQEPSRTPSAE
                     SAVDQILRLSRERPDELDLVIVGSLTNIAAAMERDPEVLARFRSVWILGSYSRRPGAP
                     EQPSSDFNTLCSPDAAARVYGSPVELNVVPIDTTFRVIFSDEHLARIRESTSPIARFV
                     DRILQCYLDFYQERMGRRTVPVHDPTVAAVLLHPDLIRTTERRRMIVEPWLGSFRAVG
                     LDHGDAGEDDDRAEMRLVTDVDAERLLDNLVDAITRPQAGFGNAG"
     gene            complement(1623..2438)
                     /locus_tag="VHR64_14490"
     CDS             complement(1623..2438)
                     /locus_tag="VHR64_14490"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304092.1"
                     /translation="MIFNSADRIRTLTPQYEGERLPDGRPFVPDDILERMKLVTNGEA
                     WGVIERGHNYHFNFEGDWINMHPDRVLVGRAVTVRYVPTRPDLNGAVQSIGESEKRSG
                     GQNTWIIDTLREGDVLVVDLFGKIEDGTFIGDNLGTAISTRAKTGLVVDGGIRDLARV
                     RELENFNCFYRGAHPSAILDVTLVEVNGPVRIGGATVMPGDVVLGTQEGVSFIPPHLA
                     ESVVTRSEDVRFRDVFGKMRIADGTYSSGDIDVSDWAEHIEADFQEWSKAAAR"
     gene            complement(2542..>2907)
                     /locus_tag="VHR64_14495"
     CDS             complement(2542..>2907)
                     /locus_tag="VHR64_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304093.1"
                     /translation="GVPTGVLMAPVLPGITDSEESIFGVAEAVADSGATTFAVLPLRL
                     DPFVREHYYSWVAQAYPELMGRYAKSFHNRHTSTAYQDRIKTISEEARERFSLNERRM
                     RGTIERPRDAGMVQLALVS"
BASE COUNT          501 a          945 c          971 g          490 t
ORIGIN      
        1 gttgaacgtg atcgtctgat cgtcgacgac ctccatcatc gatgcgtctg gcatgaagcg
       61 ggagatgaca ttgacgggcc ccatgcccat ctccaggaat cgctggaacg aggcccgcac
      121 cgactcggcg gtgcagggat cgccatcctg aaaggtgacg ccggccggaa tcttgaacgt
      181 gaacgtcgag ttgtcggcgc tcgcctccca cgactcagcc agcaccggat cgaattcggt
      241 tgtgctctcg cccttgagca tgatcaacgt ctcgtgggtg ccgaccagga tccgggcggc
      301 ggagttgctg taagcggaat gcgggtcgag gtcgggcgac gactcgtcgc caatgatcgt
      361 gaaggtgccg gttgcctggc cttgagaccg ggagagcgag cggaggctcg ccgggcgggg
      421 cgaggcgccc acttccggcg cggccgccat cagggtcgtg atcgcggcgg tcgacatgcc
      481 gagcttgaga ccgcgctcga gaacctggcg gcgggaaagg ctgccgcgcc gcgcggcctg
      541 cagcatgcgg tcgaacaatg cgtcactcat cgggggtcct ccattcagga tcgtctgcga
      601 acgaatgaaa ggtacgacgc tggaatcgta ttgaccgcgt catcttatcc cgcgttgcca
      661 aaccctgcct gcggacgcgt aatcgcgtca acgaggttgt cgagcagtcg ttccgcgtcg
      721 acatcggtga cgaggcgcat ctcggcccga tcatcatcct cgccagcatc gccgtgatcg
      781 agaccaaccg cacggaagct gcccagccac ggctcgacga tcattcgccg ccgctctgtg
      841 gtccggatca ggtcggggtg caagagcacg gccgccaccg tcgggtcgtg gaccgggacc
      901 gtgcgccggc ccatgcgctc ctggtagaag tcgaggtagc actgcaggat gcggtcgacg
      961 aaccgggcga tcggcgatgt gctctcgcgg atccgggcca ggtgctcgtc ggagaagatg
     1021 acccggaacg tcgtgtcgat cgggacgacg ttcagctcga ctgggctacc gtagacacgc
     1081 gcagctgcgt ccggactgca cagcgtgttg aagtcgctcg acggctgctc gggcgcgccg
     1141 ggccgccgcg aatagctgcc aaggatccag accgaccgga atcgcgccag cacctccgga
     1201 tcgcgctcca tcgcggccgc gatattggtc aggctaccga ctatgaccag gtcaagctcg
     1261 tccggccgtt cccgggatag ccgaaggatc tgatcgaccg cgctctcggc cgatggagtt
     1321 cgggatggct cctggacgca ggcatcgccc aggccgtccg acccgtggac atgcggcgct
     1381 gagacaggga ccgccagcgg actctccagc ccgaccgcga ccggcacttc gtccagcccg
     1441 agcacatcaa ggacccggat tgcgttgcga gcggcctgcg cggccgggca gttgccgtga
     1501 gagctgccaa tagcgacgat ctcggcatcc ggctcggccg cgattgcgac tatcatcagc
     1561 gcgtcgtcga tcccgacatc cgtatcgagg aggatctgat gacgcgccat gacgtcgatc
     1621 ccttaccggg cggccgcttt ggaccattcc tggaagtcgg cctcgatgtg ctcggcccag
     1681 tcgctgacat cgatgtcgcc cgaggagtag gtgccgtcgg cgatccgcat cttgccgaag
     1741 acatcccgga aacggacgtc ctccgaccgc gtaacgaccg actcggcgag gtgcggcggg
     1801 atgaaggaga cgccttcctg cgtgccgagc acgacatcgc cgggcatgac ggtcgcgccg
     1861 ccgatccgga ccggcccgtt gacctcgacc agcgtcacgt cgaggatggc cgagggatgc
     1921 gcgccgcggt agaagcagtt gaagttctcc agctcgcgca cgcgggcgag atcgcgaatg
     1981 ccgccatcga ccaccagtcc ggtcttggcc cgggtcgaga ttgcggtgcc gagattgtcg
     2041 ccgatgaagg taccgtcctc gatcttgccg aacaggtcca cgaccagcac gtcgccttcg
     2101 cggagcgtat cgatgatcca ggtgttctgg ccgccggacc gcttctccga ctcgccgatc
     2161 gactggacgg ccccgttcag atcgggtcgg gtcgggacat accggacggt cacggcgcgg
     2221 ccgaccagaa cgcggtcggg gtgcatgttg atccagtccc cctcgaagtt gaagtggtag
     2281 ttgtgaccgc gctcgatcac gccccacgcc tcgccgttgg tgacgagctt catccgctcg
     2341 aggatgtcat ccggcacaaa cggccgaccg tcgggcagcc gctcgccctc gtattgcggg
     2401 gtcagcgtcc gaatccgatc tgccgagttg aaaatcatgc ctgcggtgct ccctgaacct
     2461 ctcccgtgac ggcagccgtt gccgtcgcgg tagcctactc gaacccccga gaaaccgccc
     2521 ggccgcgatg cacgactggt ttcaggagac cagcgcgagc tggaccatcc cggcgtcgcg
     2581 gggccgttcg atcgtgccgc gcatgcgccg ctcgttcagg ctgaaccgct cgcgcgcctc
     2641 ctccgagatc gtcttgatgc ggtcttggta tgcggtggaa gtgtggcgat tgtggaagga
     2701 cttcgcgtac cggcccatta gctcgggata ggcctgcgcg acccacgagt agtagtgctc
     2761 gcggacgaag gggtcgaggc ggaggggcag gaccgcaaac gttgtcgcgc cggaatcggc
     2821 cacggcctcg gcgacaccga agatcgattc ctcgctgtcg gtaatcccgg gcaggactgg
     2881 agccatcagc acgccagtcg ggacccc
//
LOCUS       DATAOZ010000576        11512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5220927, whole genome shotgun sequence.
ACCESSION   DATAOZ010000576 DATAOZ010000000
VERSION     DATAOZ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11512
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5220927"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VHR64_14500"
     CDS             <1..1615
                     /locus_tag="VHR64_14500"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX3304094.1"
                     /translation="TSWRPVRAATLAGLAIVLSLLMIRTTVELSFYRIDTGEELLAQE
                     MAAADLRDIAARIANLSRDVNGTERSPSNPPGGKEMSIALDRTVQWPFRWYFRDFPNM
                     VITAPGDATTKEAQLVIAPDPTGMTEAGYQPRTVNAVTTVPAEYLNPSLATVFKYTFV
                     PANWDQGLRFMLYRDLIDTTAPRTVIFGYSGQVVSKMTGERPTYKLSDRAGAGSNPGQ
                     FNQPRGVAVSPDDARVYVLDTQNGRIEVFSTDTGELLGIWGDGEGDQVTLSLTDNGLG
                     PYGIATGPEGNVYVADTWNHRIVVVNPDGQVVRTFGDFANNEDSTDPQQNPGKFYGPR
                     GLVVYENELYVTDTGNERVEVFGLDGAFHRAWGGTGSAPNQFIEPVGIAVSPAGQVFV
                     ADSGNARISIFDTQGKPVAQWAVPQWQGQQFYEPYLAFDPNGLLYASSAPTSSVIVFG
                     SDGEQQDVLTDAEGVALQMPAGMAVSSTNELYIADRGSSEVYSADLGPVTGAGEVTSL
                     PVASPEGSPQASPIASPQASPEASPSASG"
     gene            1621..3072
                     /locus_tag="VHR64_14505"
     CDS             1621..3072
                     /locus_tag="VHR64_14505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3304095.1"
                     /translation="MVHLQPVNTPRTKSLWLDEALAVEPCAEQAEPLQGSHICDICIV
                     GGGYTGLWTALRIKELDPSVSVTLLEAKMCGSGASGRNGGMIGCWWDKLGTLIKVCGT
                     DDGLFMAQAAYDAIGEIEQFLTDNQIEAHFVRKGRLQAATSPFQLDSWEPALQLAEKL
                     GFGWYYTRLTRDEIQHRGGSPHYIGGCYRDGDATVQPALLARGLRKVAIERGIQIFEN
                     TPVTQIHEGDPVVLRIPNGAVMARKVVLATNAWTAAIPELRRHMIVVSSDIVTTAPIP
                     DRLPEIGWTGGESLADCRLMVHYMHVTHDKRISIGRGSGALAYLGRVTPAFDGNQAKA
                     KVVEQGLRKFYPMLDDVPVTHAWGGPIDRTRSGTFAFGRLTGSPNIAYGIGYSGSGVG
                     QSVVGGKILASTALDRVDEWSTSRLNQGPVILYPPDPIKFFAGIAVRGVLTHREEGEE
                     DGRAPHPLVKSISKLAYPTLPRHLNRAKTAQTT"
     gene            complement(3253..4473)
                     /locus_tag="VHR64_14510"
     CDS             complement(3253..4473)
                     /locus_tag="VHR64_14510"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3304096.1"
                     /translation="MRPRSRAIETVINRRALLGGGLTLTLAGCGGGDNGSETSVPTVE
                     PTSTTVPVPTQEPIGSPVAGYSQPNKWADRTLNVAAWGGNIQDAQATAFFDPFEEATA
                     ASIQIKTADLGRLKSQVESEDVSWDVLTLPAADALALARTNYLEPIDFAVVDKSPLVP
                     EIVLQYAVGEAFYSTVIVFRADRPTPPRDWTEFWTFTPRESPDDPIPPEDLRTLARNP
                     VGTLEFALLADGVAKDALYPLDLERAFASLDKVRDYIVAWYEDGKQPIELLVADQVAI
                     GSAWNTRAWQLNVTPDIGTQWNGGMLSADMWVVPKGAPNKDVAMDFINFATRAIPSAN
                     FSRLVPFGPVNLEAPPLLDPERLKEMPSAPENFDVQFVQNWVWWADNIEAVTQRFEDW
                     LLTKPESSPEAESH"
     gene            4638..6155
                     /locus_tag="VHR64_14515"
     CDS             4638..6155
                     /locus_tag="VHR64_14515"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX3304097.1"
                     /translation="MTIRIAVGGIVHETNTFAHPPTDLAEFKANGLYEGEEMRSLVGT
                     NTVVGGAIEAIDADPGLEMIPTAFSHAIPGGTVTRAAFDYIVDQITSGIAASKPDAVV
                     LDLHGALVVDGFPGGDGEVARRVREIVGPDVPIVAPLDLHGNIDDVLVANADILLPYH
                     TYPHVDTAERGREAVELAVRMARGEIKPTMRAVKLTISPPGPQQYSKFEPTVSLQQIA
                     RDWETKPGVIDTSILFAFPYSDVPHNGMAVVATTDNDPALADQICEELGREIIRRHRE
                     FWPEMLTVEEAVHAAMSEPKGPVVLADYGDNPGGGSSCDGTAILWGLLDLGAQGAAVG
                     HICDPAVAAIAHEAGGGAEIEVELGGKRDNLHGAPIPVKATVLRLSNGDYVYEGPMNR
                     GVHNTMGPTAVLACRGRHGNVVEVIVSSRRVQALDTAIFRSQGIEPTERKILVVKSSV
                     HFRGAFTPIAARIILVNTPGLLQLDVSAFPFEHITRPLWPHDQDEPRMIKMNTPG"
     gene            6205..6471
                     /locus_tag="VHR64_14520"
     CDS             6205..6471
                     /locus_tag="VHR64_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304098.1"
                     /translation="MDTYNVYMDEASADGLNGESEVDVNFRIVAASTDTEDAEENAVI
                     VGLDLIDLMNLRDALQNEIDSFTLLQFEANALASTDELEDEDLP"
     gene            complement(6741..7481)
                     /locus_tag="VHR64_14525"
     CDS             complement(6741..7481)
                     /locus_tag="VHR64_14525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX3304099.1"
                     /translation="MQSSANDVWKNDWVVRRFLEGVRGGIPYANDQLEVMLRLLDAAE
                     RPINSFLDLGSGSGLLAIAILTRHPEARATLVDFSEPMMEAARDLLGEDTNLPSFVLA
                     DLNSPDWVKAVEGEGPYDAIVSGFTIHHLQHDRKAALYQELLPLLAPGALFINVEHVA
                     SSSTWLEAVWNEAMIDSLTEYHEKQRTGQSREMIAREYGSRPDQEANVLASAETQLRW
                     LREAGFADVDCVFKYFELAVLTGRRPVD"
     gene            complement(7510..8136)
                     /locus_tag="VHR64_14530"
     CDS             complement(7510..8136)
                     /locus_tag="VHR64_14530"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX3304100.1"
                     /translation="MDWVVLPKGVLLGLSVAAPVGPIGVLTIRRTIGDGFRMGFATGL
                     GAATADATYGLVAAFGLTAIMAVLTEHGDAIRLVGGVMLLAIGLRGIWQSRVAGSVQA
                     ADVGRVLTPAGSYAQTVGLTLTNPMTIIAFIGMFAGVGISSGGADVPSALALVIGVAV
                     GSAAWWLFLAFTVSRIRHRLSPSAVQLINFASSVIIVAFGMAAVIAAR"
     gene            complement(8161..10296)
                     /gene="ppk1"
                     /locus_tag="VHR64_14535"
     CDS             complement(8161..10296)
                     /gene="ppk1"
                     /locus_tag="VHR64_14535"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX3304101.1"
                     /translation="MTETIDQHVEPLEEPTVRVPPELYLNRELSWLAFNERVLEEAED
                     PANPLLERVKFLSIFSSNLDEFFMIRVANLKRKLTAGIIDPGPDGLPPQALAPRLRDE
                     TQRLLTRQHEVFAQTILPELCICGIDILSVNDLEPEDRAALSSLFVRDLFPILTPQAI
                     DRGRRFPHVSNQSLNLLVQLHGEDPEPRYARVKIPAVLDRLVRVPSSEYVGTGGRFVW
                     LEELVAAEIVKLFPGNAVIAVYPFHVNRDSDIEFDDTDDDAIDLLHLVERQLSQRTFG
                     VVTQIVVDVSMPEAVRSWLVSQLHATERDLYVVDGPLALEGLVCLLSLERPDLLDPPL
                     VPASVHVMDGTTSEPIDWNEADVFAAIRHQDVMVQRPYQGFSTLTQFLGTAARDPDVV
                     AIKQTLYRLGKNSPIIPLLMEARDDDTQVAVLMEVKARFDEENNIALAHELETAGVHV
                     NYGLAGLKTHCKALLVVRREADGLRRYVHLSTGNYNAATSRIYEDIGIITANEDIAAD
                     VTELFNALTGYSRQDTYRTLIVAPPNMRGAIIERIDREIEQHKAQGGGRIIFKMNSLV
                     DREVIRALYAASTAGVEIDLIIRGICCLRPSVPGWSQNIRVRSLVGRFLEHSRIYYFR
                     NGGDDQIFLGSADMMERNLDRRVEVLFPILDPAHKSRIRDEILPAYLRDTANSRVLKA
                     DGTYQRVCQQPGATGEFDVQRWLIDFYRR"
     gene            10591..>11512
                     /locus_tag="VHR64_14540"
     CDS             10591..>11512
                     /locus_tag="VHR64_14540"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3304102.1"
                     /translation="MTDGNGYLAPGYDVEKVRDAIKDNVWLHYTNMKAVRESPEKPLV
                     LVKGDGSTVWDSDGNAYLDCLAGIYSVNAGYGRREIAEAMMAQLEQIPFINPFGFTSV
                     PTAVFADRLADLVPLGGDARIFFTSGGGEAVETALKIAKKYQTVKGFPHRYKTISRRT
                     AWHGTTFGALSVNGLTDARNGFGPLMPGARHAPMSHRYRCPYCQHDSACNSTCYDEVE
                     RLVEFEGPETVACIIMEPVQNAGGCIPPGSQDYFKKIRKLCDETGILMIMDEVICGFG
                     RLGELFASTYYGVMPDIITTAKGITSSYAPL"
BASE COUNT         2184 a         3808 c         3531 g         1989 t
ORIGIN      
        1 gacaagctgg cgaccggtcc gcgccgccac cctcgccggg ctcgcaattg tgctctcgct
       61 cctgatgatc cgaacgacgg tcgagctcag cttctaccgc atcgacaccg gcgaggagct
      121 gctggcgcag gagatggccg cagccgacct gcgcgacatc gccgcccgga tcgccaacct
      181 ctcgcgcgac gtcaacggca ccgagcgctc gccgtccaac ccgccaggcg gcaaggagat
      241 gtcgatcgcg ctcgaccgga ccgtgcagtg gcccttccgc tggtacttcc gcgacttccc
      301 gaacatggta atcaccgcgc cgggtgacgc gacgaccaag gaagcccagc tcgtgatcgc
      361 gcccgatccg accggtatga ccgaggctgg ctaccagcca cgcacggtca acgcggtcac
      421 aaccgtgccg gccgagtatc tgaacccatc gctcgctacc gtgttcaagt acaccttcgt
      481 gccggccaac tgggaccagg gcctgcgctt catgctctat cgcgatctga tcgacacgac
      541 cgccccccgc accgtcatat tcggctattc cggccaggtc gtcagcaaga tgaccggcga
      601 gcgtccgacc tacaagctct ccgaccgggc cggcgccggc tccaatccag gccagttcaa
      661 ccagccacgt ggcgtcgcgg tgtcgccgga tgatgcccgc gtctacgtgc tcgataccca
      721 gaatggccgg atcgaggtct tctctaccga caccggcgag ctgctcggaa tctggggcga
      781 cggcgaaggc gaccaggtca ccctctcact gaccgataac ggcctcggtc catacggcat
      841 cgcgaccggt ccggaaggga atgtctacgt cgccgatacc tggaaccacc ggatcgtcgt
      901 tgtcaaccct gacggccagg tcgtccgcac cttcggcgac tttgccaata acgaggactc
      961 gaccgatccg cagcagaacc cgggcaagtt ctatggcccg cgcggacttg tggtctacga
     1021 gaacgagctc tacgtgaccg acacgggcaa cgagcgcgtc gaagtcttcg gacttgatgg
     1081 cgcgttccat cgtgcctggg gcggcaccgg ctccgctccg aaccagttca tcgaaccggt
     1141 cggaatcgcg gtcagccccg ccggccaggt tttcgtggcc gactcgggca acgcgcggat
     1201 cagcattttc gacacccagg gcaaaccggt cgcgcagtgg gcagttcccc agtggcaggg
     1261 acagcagttc tacgagccgt acctcgcctt cgatccgaac ggcctgctct acgcatcttc
     1321 tgcgccgacc tcgtctgtca tcgtcttcgg atcggacggc gagcagcagg atgtcctgac
     1381 ggatgcggag ggtgtcgccc ttcagatgcc ggcgggcatg gcggtgtcgt caaccaacga
     1441 gctctatatt gccgaccgtg gcagcagcga ggtctactcg gctgaccttg ggccggtgac
     1501 cggcgccggt gaggtaacct cgcttccggt cgccagccca gagggcagcc cgcaggcgtc
     1561 gccaatcgcg tcaccgcagg cttcgcccga ggcctctcca tcagcgagcg gatagcacca
     1621 gtggtccact tgcaaccagt caacacgcct cgcacgaaat cactctggct cgacgaagcg
     1681 ctggcggtcg agccatgtgc tgagcaagcg gagccgctgc agggctcgca catctgcgat
     1741 atctgcatcg tcggcggcgg ctacaccggg ctctggactg cgctccgcat caaggagctc
     1801 gatccctcgg tctccgttac gctgctcgag gcgaagatgt gtggtagcgg cgccagcggc
     1861 cgcaacggcg gcatgatcgg ctgctggtgg gacaagctcg gcacgctgat caaggtctgc
     1921 ggtaccgacg acggcctctt catggctcag gccgcctatg acgcgatcgg cgaaatcgag
     1981 caattcctga ccgacaacca gatcgaggcg cacttcgtcc gcaagggccg gctccaggcc
     2041 gcgacgtcac cattccagct cgactcgtgg gaaccggcgc tgcagttggc cgagaagctg
     2101 ggtttcggct ggtactacac tcgcctgacg cgcgacgaga tccagcaccg tggcggatcg
     2161 ccgcactaca tcggcgggtg ctaccgggac ggcgacgcca ccgttcagcc ggcactgctc
     2221 gcgcgcgggt tgcgcaaagt cgcgatcgag cgtggcatcc agatcttcga gaacacgccg
     2281 gtcacgcaga tccacgaggg cgatccggtc gtcctgcgca tccccaatgg cgccgtcatg
     2341 gcccgcaagg tggtgctcgc gaccaacgcc tggaccgcgg ctattccgga gctgcggcgg
     2401 cacatgatcg tcgtctcgtc agacatcgtc accaccgcgc cgattccgga ccgtctcccg
     2461 gagattggct ggaccggcgg ggagtcgctc gccgactgcc ggctcatggt ccactacatg
     2521 cacgtcacgc acgacaaacg gatcagcatc ggtcgcggca gcggcgcgct ggcctacctc
     2581 ggtcgagtga cgcctgcctt cgacggtaat caggccaagg ccaaggtcgt cgagcagggg
     2641 ctgcgcaagt tctacccaat gctggatgac gttccggtca cccacgcttg gggcggtccg
     2701 atcgaccgca cccggtcggg cacgttcgcg ttcggtcggc tgaccggctc gccgaatatt
     2761 gcctatggca tcgggtactc gggatcgggc gtcggccaaa gcgtggttgg cggcaagatc
     2821 ctggcttcga ccgcgctcga tcgcgtcgac gaatggtcga ctagccgcct caaccaggga
     2881 ccggtgatcc tctacccacc tgacccgatc aagttcttcg ccggaatcgc ggtccgtggc
     2941 gttctgaccc accgcgagga aggcgaggag gacggccgcg ccccgcaccc gctcgtcaag
     3001 tccatctcga aactcgccta cccaaccctg ccccgacacc tcaatcgcgc aaaaaccgca
     3061 cagacgacgt agcaccggcc cccgtggccg gcttcccccc aacgggcagc gaactcagtt
     3121 aagccgatca gacacagagg aatgtctcag aaaacgtagt tccgggcggc tcgcccggca
     3181 tcttgcgacc ggaggaccgg gcgagccgcc cggaactaca tccctggacc tgcacctcga
     3241 ctgaggcgtg gtctaatggc tctcggcttc aggcgacgac tccggcttgg tcagaagcca
     3301 atcctcgaag cgctgagtca ctgcctcgat gttgtccgcc caccagaccc agttctggac
     3361 gaactggacg tcgaagttct cgggggcgct cggcatctcc ttcagtcgct cggggtcgag
     3421 caacggtggc gcctcgagat tgaccggccc gaacggaacg agccgggaga agttggccga
     3481 tgggatcgca cgggtcgcga agttgatgaa gtccatcgcg acatccttgt tcggcgcgcc
     3541 cttgggaacc acccacatgt cggctgagag catgccgccg ttccattggg ttccgatgtc
     3601 gggcgtgacg ttcaactgcc aggcgcgcgt gttccacgcg ctgccaatcg ccacctggtc
     3661 tgccacgagc aactcgatcg gttgcttccc gtcctcgtac caggccacga tgtaatcgcg
     3721 aaccttgtcg agtgacgcga aggcccgctc caggtcgagc ggatagagcg cgtccttcgc
     3781 cacgccgtcg gccaggagcg cgaactccag cgtgccgacc gggtttcggg ccaacgtgcg
     3841 caggtcttcc ggcggaatcg gatcatcggg cgactcgcgc ggggtgaagg tccagaactc
     3901 ggtccaatcg cgtggcggag tcggacgatc ggcgcggaag acgatcacgg tcgaatagaa
     3961 cgcctcgccg accgcgtact ggagcacgat ttcggggacg agcggcgact tgtcaacgac
     4021 cgcaaaatcg atcggctcga ggtaattggt tcgggccagg gcaagagcgt ccgcggctgg
     4081 cagcgtcagg acgtcccatg acacatcttc ggattccacc tgggatttga gccgcccaag
     4141 atcggctgtc ttgatctgga tagacgccgc ggttgcttcc tcgaacggat cgaagaacgc
     4201 ggtcgcctgc gcgtcctgga tattgccgcc ccaggccgcg acgtttagtg tccggtcggc
     4261 ccacttgttg ggctggctgt aacccgcgac cggcgatccg atcggctcct gggtcgggac
     4321 cggcaccgtg gtgctggtcg gctcgactgt cgggaccgac gtctcggacc cgttgtcgcc
     4381 tccaccacac ccggccagcg tcaacgtcag cccgccgccg agcagtgcgc gccggttgat
     4441 caccgtctcg attgcacgac tacgaggtct cacggttccc ccgactcgcc ggtcacggcc
     4501 aggacacatc ggcccattcg agcgcacatt atcgtcgatt ggcgacgtga tgatggattt
     4561 ctcagtcatc aaccgaaata cccgtcgtcc ctgataggat gacgcattcg acaagcagta
     4621 tttcgaccgg ggggacaatg acaattcgca tcgctgtcgg cggcatcgtc cacgagacca
     4681 acaccttcgc acacccgcct accgacctcg ccgagttcaa ggcgaacggg ctctacgagg
     4741 gcgaggaaat gcgctcgctg gtgggtacca ataccgtcgt cggaggcgca attgaggcaa
     4801 tagatgccga tcccggtctc gagatgatcc cgacggcgtt cagccacgcg attcccggag
     4861 gaaccgtcac ccgggcagcc ttcgactaca tcgtcgacca gatcacgtcc ggaatcgctg
     4921 catcgaagcc ggatgcggtt gtgctcgacc tgcacggcgc gctggtggtc gacggattcc
     4981 cgggcggcga cggcgaggtt gcacggcgcg tgcgggaaat cgtcggaccg gacgtgccga
     5041 tcgtcgcgcc cctggacctg catggaaaca tcgacgacgt cttggtcgcg aacgctgaca
     5101 tcctgctgcc gtaccacacc tacccgcacg tcgacaccgc cgagcgtggc cgggaagcgg
     5161 tcgagttggc agtgcgaatg gcgcgcggcg aaatcaaacc gacgatgcgc gcggtcaagc
     5221 tcacaatctc gccacccgga ccgcagcaat acagcaagtt cgagcccacg gtctcgctcc
     5281 agcagattgc ccgcgactgg gagacgaagc ccggcgtgat cgatacctcg atcctgttcg
     5341 ccttccccta ctccgacgtg ccgcacaacg ggatggccgt cgtcgccacc accgacaacg
     5401 acccggcgct cgccgatcaa atttgcgagg agctcggccg cgaaattatc cgccgccacc
     5461 gagaattctg gccggagatg ttgaccgtcg aagaagcggt ccatgccgcc atgtcggagc
     5521 cgaagggacc ggtcgtcctc gccgactacg gcgacaatcc gggcggcggc tcgtcctgtg
     5581 acggcacggc catcctctgg gggctgctcg atctcggcgc gcagggtgcg gcagtcgggc
     5641 acatctgcga tccggcagtg gcggcgatcg cgcacgaggc cggcggaggc gcggagatcg
     5701 aggtcgagct cggcggcaag cgggacaacc tccacggcgc gcccatcccg gtcaaggcaa
     5761 ccgtcctgcg cctctcgaac ggcgactacg tctacgaagg accgatgaat cgcggtgtcc
     5821 acaacacgat ggggccgacg gccgtcctcg cctgccgcgg gcggcatggc aacgtcgtcg
     5881 aagtgatcgt tagctcgcgc cgggtgcagg cgctggacac agccatcttc cgctcccaag
     5941 gcatcgagcc aaccgagcgc aagatcctcg tcgtcaagtc gagtgtccat tttcggggcg
     6001 cattcacgcc gatcgccgcg cgcattatcc tggtcaacac cccgggtctg ctccagttgg
     6061 atgtgtcggc attcccgttc gagcacatca cacgaccgtt gtggccgcat gatcaggacg
     6121 aaccgcgtat gatcaaaatg aacacgcccg gatagccggc gccgaccgat cgcgcgaact
     6181 tgctgaaccc gaaggagagc tgcgttggac acgtacaacg tctatatgga tgaggcttcg
     6241 gcggacggct tgaacggcga atccgaggtc gatgtcaatt ttcggatcgt tgcggccagc
     6301 acggataccg aggacgccga ggagaacgcg gtcatcgtcg ggctcgatct gatcgacctg
     6361 atgaacctgc gcgacgcgct tcagaacgag atcgacagct tcacgctgct ccagttcgaa
     6421 gcgaacgcac tagccagcac cgacgagctc gaagacgaag acctcccgta acggccgtgg
     6481 aaattgaacc ctgaacgtag cgccgacccc ccgtggccgg ctttccgttg tcaggtgtac
     6541 gacctaacgc ggtttccgaa tcacatgacc gttgaaccaa cggagcgcgg ggttacaacc
     6601 ccccgcttct ctcattcgcc gcgagggtgc cggtggaatg cggttgctcg ggatggttcc
     6661 cgcgccgaac ggaccgaagc gggtcctggc ggggcgtggt aaccccgcgc tacgtaatga
     6721 cgaataagcc cgaagtcgtt ttaatccacc gggcggcggc cggtcagaac ggccagctcg
     6781 aaatacttga aaacgcagtc aacgtcggcg aaaccggctt cgcgcagcca gcgcagctgg
     6841 gtctcggccg aggccagcac gttcgcttcc tggtcgggtc gactaccgta ctcgcgggcg
     6901 atcatctcgc ggctctgccc ggtccgctgc ttctcgtggt actcggtcaa cgagtcgatc
     6961 atcgcctcgt tccagaccgc ttcgagccat gtcgaggacg aggcgacgtg ctcgacattg
     7021 atgaagagcg cgcccggcgc gagcaatggc agcagctcct ggtagagcgc cgccttgcga
     7081 tcgtgctgga ggtgatggat ggtgaagcct gagacgattg cgtcgtatgg accctcgccc
     7141 tcgacggcct tgacccaatc cgggctgttg agatccgcca gcacgaacga tggcaaattg
     7201 gtgtcttcgc cgaggagatc ccgcgcggcc tccatcatcg gctccgagaa atcgaccagc
     7261 gttgcccgcg cctcggggtg acgggtcagg atcgcaatcg cgagcagtcc cgagccgctc
     7321 ccgagatcga ggaagctgtt gatcggtcgc tcagccgcat ccagcagccg cagcatgacc
     7381 tccagctgat cattggcgta ggggatgcct ccccgcacgc cctcgaggaa gcgccggacc
     7441 acccaatcgt tcttccagac atcgttcgcg ctcgactgca tcgcaccttt ccatcacgca
     7501 cgagcaagct taccgagccg cgatcactgc cgccatcccg aaagcaacga ttatcacgct
     7561 cgaggcgaaa ttgatcagtt gaaccgcgct cggcgagagt cgatgccgga ttcgggaaac
     7621 ggtgaaggcg agaaagagcc accatgcggc ggagccgacc gcgactccga tcacgagcgc
     7681 cagcgcactc ggaacatccg cgccgccgga gctaatcccg actcccgcga acatcccgat
     7741 gaaagcgatg atggtcatcg gatttgtcag cgtcaggccg acagtctgcg catacgaccc
     7801 cgccggagtc agcacgcgcc caacgtcggc tgcctggacc gaaccggcaa cccggctctg
     7861 ccagattccg cgaagcccga tcgccagcag catcacgcca cccacgagcc ggatggcgtc
     7921 gccgtgctcg gtcaacaccg ccatgattgc cgtcagcccg aacgccgcaa ccagcccgta
     7981 ggtcgcatct gcggtcgctg cgccgaggcc ggtcgcgaaa cccatcctga acccgtcgcc
     8041 gatggttcgg cgaatcgtaa ggacgccgat cggtccaacc ggcgccgcga ctgacaatcc
     8101 gaggagcacg cccttcggca agacaaccca gtccatctat tttcctgcta atgaatcccg
     8161 ttaccgacga tagaagtcga tcagccaccg ttgaacatcg aattccccgg tggcgccggg
     8221 ttgctggcag acacgctggt acgttccgtc ggccttgagc acgcgcgagt tggcggtatc
     8281 gcgcaggtag gcaggcagaa tctcgtccct gatacgggac ttgtgcgccg gatcgaggat
     8341 tgggaagaga acctcgacac gtcggtccag gtttcgttcc atcatgtccg cgctgccaag
     8401 gaagatctga tcatcaccgc cgttcctgaa atagtagatc cggctgtgct cgaggaaccg
     8461 gcccacgagg ctgcgcacgc ggatgttctg gctccagccc ggcacactcg gtcgcaggca
     8521 gcaaatgccg cggatgatca aatcgatctc gacacccgcg gtcgaggcgg cataaagcgc
     8581 ccttatcact tcacgatcga ccagcgagtt catcttgaag atgatccgac cgccgccctg
     8641 tgccttgtgc tgctcgatct cgcggtcaat tcgctcgatg atcgccccgc gcatgttcgg
     8701 cggagcaacg atcagggtcc ggtaggtgtc ctgccgggaa tacccggtca gcgcgttgaa
     8761 cagctcggtg acatcggccg cgatatcctc gttcgccgtg atgatcccga tatcctcgta
     8821 aatcctggac gtcgccgcgt tgtagttccc ggtcgagaga tgcacgtacc gccggagccc
     8881 atccgcttct ctccgcacga ccagcaacgc cttgcagtgc gtcttgagcc cggccaaacc
     8941 gtaattgacg tggacgccgg ccgtctcgag ctcatgcgcc agcgcgatgt tgttctcctc
     9001 gtcgaagcga gccttaacct ccatcagcac ggcaacctgc gtgtcgtcgt cccgcgcttc
     9061 catgagcagc gggatgatcg gcgagttctt gccgagtcga tagagcgtct gcttgatcgc
     9121 tacgacatcc ggatcccgcg ccgcggtgcc caggaactga gtgagcgtgg aaaacccctg
     9181 gtatggccgc tgcaccatca cgtcctgatg tcgaatcgcc gcgaagacat ccgcctcgtt
     9241 ccagtcaata ggctccgatg tggtcccatc cattacatga actgacgccg gcacgagagg
     9301 cggatcgagc agatccggcc gttccagcga caacaggcag accaggcctt cgagcgcgag
     9361 cggtccatcg accacgtaga ggtcccgctc cgtggcatgg agctggctca cgagccagga
     9421 gcgaacggcc tctggcatcg acacatcgac gacgatctgg gtaacgacgc caaaggtgcg
     9481 ctgcgaaagt tggcgctcga cgagatgcaa aaggtcgatc gcgtcatcgt cggtatcgtc
     9541 gaactcgatg tcggagtcgc ggttgacatg aaacgggtat accgcgatga ctgcgttgcc
     9601 cgggaacaac ttgacgatct cggccgcgac gagttcttcc agccagacaa atcgcccgcc
     9661 cgtcccgaca tattcggagg acggaacgcg aaccagtcga tcgagcaccg ccgggatctt
     9721 cacccgggcg tacctgggct cgggatcttc gccatggagc tgcacaagca gattcaggct
     9781 ctggttcgag acatgcggaa atcgccgtcc ccggtcgatc gcctggggag tcagaatggg
     9841 gaaaaggtcc cgcacaaaga gtgagctgag cgcggcgcga tcctccggtt caaggtcgtt
     9901 gacagacagg atatcgatgc cgcaaatgca cagttccggc aggatcgtct gggcgaatac
     9961 ctcatgctgc cgggtgagca accgctgcgt ctcgtcccgg agccggggag cgagcgcctg
    10021 cggcggaagc ccgtccgggc cgggatcgat gatgcccgcg gtgagcttgc gcttcaggtt
    10081 ggcgacccgg atcatgaaga attcgtcgag gtttgaagag aagatcgaca ggaacttgac
    10141 ccgctccagc agcggattcg ccggatcctc tgcctcctcc agcacacgct cgttgaacgc
    10201 gagccaactc agctcccggt tcagatagag ctctggcgga acacggacgg tgggctcctc
    10261 taatggctca acgtgttgat ctattgtctc ggtcatatat cggccgactc cagaaccgga
    10321 aacccggcgc cgcgctcacc agacttcctc cttcgatcat acacgcgtat cgggcgattg
    10381 gaaccggtct ggtttccggt gtcgacaggc ttgccgcgac ttcggtatgc tgcccgcgga
    10441 ccttgaatat tcgtaccgat aaccatcgag cgcatgccca tgccactcct gcttcacgac
    10501 tactggaaat ccaccggggc atctccgcca gacctccgct gaaggtctcc ctcattctcc
    10561 tgccaatagt tcacatgaaa ggccgctcac atgaccgacg gtaacggtta cctggccccc
    10621 ggctacgacg tcgaaaaggt ccgggatgcc atcaaggaca atgtctggct ccattacacg
    10681 aacatgaagg cggtgcgcga gtcaccggag aagccgcttg tcctggtcaa gggcgacggc
    10741 agcacggtct gggactcgga cggcaacgcc tatctcgatt gcctggccgg gatctactca
    10801 gtcaacgccg ggtacgggcg ccgcgagatc gctgaggcga tgatggcgca gctcgagcag
    10861 atcccgttca tcaacccgtt cggcttcacc agtgtgccta ccgcggtctt tgccgatcgc
    10921 ctggccgatc tcgttccgct cggcggggac gcgcgaatct tcttcacctc gggcggcggc
    10981 gaggcggtcg agacggcgct caagatcgcg aagaagtacc agacggtcaa gggtttccca
    11041 caccggtaca agacaatctc acgccgcact gcctggcacg gcaccacctt tggcgcgctc
    11101 tcggtcaatg gcctgaccga cgcacgcaac ggcttcggcc cgttgatgcc gggcgcgcgg
    11161 cacgcgccga tgtcgcaccg gtatcgctgc ccctactgcc agcacgattc ggcctgcaac
    11221 tcgacctgct atgacgaggt cgagcggttg gtcgagttcg agggcccgga aacagtcgcc
    11281 tgcatcatca tggagccggt ccagaacgcc ggcggatgca tcccgccggg atcgcaggac
    11341 tacttcaaga agatccgcaa actctgcgac gagaccggca tcctgatgat catggacgag
    11401 gtcatctgcg gtttcggacg gctcggtgag ctgttcgcct cgacctacta cggggtcatg
    11461 ccggacatca tcacgaccgc aaagggcatc acgtcgtcgt acgcgccgct tg
//
LOCUS       DATAOZ010000577         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3908549, whole genome shotgun sequence.
ACCESSION   DATAOZ010000577 DATAOZ010000000
VERSION     DATAOZ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3908549"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VHR64_14545"
     CDS             complement(<1..844)
                     /locus_tag="VHR64_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3304103.1"
                     /translation="MASVGAAHGEIGLEHGGRSSTRVSLAGLGYTMLLCVVAFLIIVP
                     IALLIIHSFEVSAFSPSPTWGIDNWRTALAEPRILDALWTTVSLAITRQAIAVVLGVV
                     LAWLIARTDLPGRDWLELGFWITVFLPTLTVVLGWIMVFDNFKGLANKLLELLPFVEK
                     GPFDIFSWWGIVWVHLVTATLPIKVMLLTPAFRNLDATLEEASRTSGAGPLGTLARVG
                     LPLMAPTILVVTILGIIRSMEAFEIELILGYPAKIDVYSTLIYRQVMQSPPEYGRATA
                     LSMVI"
     gene            complement(1010..1765)
                     /locus_tag="VHR64_14550"
     CDS             complement(1010..1765)
                     /locus_tag="VHR64_14550"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX3304104.1"
                     /translation="MTYGWRAKVGIIVPSGNSVSEPQFRMMAPPGVEFFAARLRLERE
                     GADRYLKMAEGIEDAAGLLADLRPSFILFHCTAVSVAGGVGFDDQIAARIERSTGYPG
                     GATVSGVVQALHALDARRIALITPYAASTNALEVAFLEGHGFEVTTNVALDTLRERRQ
                     QEPGEWYRIVREHYTAEADAVFLSCTAVRTIEVIDWLEQDLGVPVVTSNQGALWYVLR
                     RLGIRDRVEGYGRLLREYPELPASAQLVPSLTA"
     gene            complement(2015..>3010)
                     /locus_tag="VHR64_14555"
     CDS             complement(2015..>3010)
                     /locus_tag="VHR64_14555"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3304105.1"
                     /translation="AAKKEGVLSINHQPSSLLQNDVLGAFKEKYPWLKVEATSAPRGE
                     FANRVLAEQRNGQYLWDVEILGGFGGSNVQFVNADALQDMRPLLARLPKEITDDSKWA
                     GGFEMWAGPEHTHSLITQIQLQTGVSVNRSKIPTSELSSIDQILDPKFKGKIVTYSPE
                     RIGGATYTLAAVSMVKGEDFIKKLFFDQAVVALADQRQLAERVATGQYPIGLGASETD
                     YDEYKAKGIGLEVEEIREVATTYLRASGITYFKNAPHPNAANVFMHWFLSQEGQDAWA
                     SKGGPIAVTRRLDVPVYHPKAMFDPSQLKDYKAILGTVESQEWLDRTSQIINTKP"
BASE COUNT          550 a          964 c          930 g          566 t
ORIGIN      
        1 ggatcaccat gctaagcgcg gtcgcgcgcc cgtactccgg cggggattgc atgacctgcc
       61 gatagatgag cgtgctgtac acgtcgatct tcgcgggata gccgaggatc agctcgatct
      121 cgaacgcttc catggatcgg atgatcccga gaatcgtgac gacgaggatg gtcggcgcca
      181 tcagggggag cccgacgcgt gcgagcgtgc ccaatgggcc ggcgccggac gtccgtgacg
      241 cttcttccaa cgtggcgtcc aggttccgga atgccggggt gaggagcatg accttgatcg
      301 gcagcgtcgc cgtcaccagg tggacccaga cgatccccca ccaggagaag atatcgaacg
      361 gtcccttctc gacgaatggc agcagctcga gcagcttgtt cgccagcccc ttgaaattgt
      421 cgaaaaccat gatccagccc agcaccacgg tcagtgtcgg caggaagaca gtgatccaga
      481 aaccgagctc cagccaatca cggcccggca gatcggtgcg ggcgatgagc caggcgagga
      541 caactcccag gacgaccgcg atggcctgac gcgtgatcgc gagactgacc gtcgtccaca
      601 acgcatcgag aatcctcggt tcggcgaggg cggtgcgcca gttgtcgatt ccccaggtcg
      661 gcgacgggct aaatgcactg acctcgaagc tgtggatgat caggagcgcg atcgggacga
      721 tgatcaggaa cgcgaccacg cagagcagca tggtgtatcc gagaccggcg agcgataccc
      781 gggtcgacga tctgccccca tgctcgaggc caatttcccc gtgcgcggct cctacgctcg
      841 ccatgccagc caatcgcccg cgggtggatc tggcttcact gtcgctcctc gggtccgacg
      901 gtcatccctg accgatcacc tagggccggc tgcctttgta catctccgga tcgtcgcttt
      961 cgaccgcgcc gtgcgtgcat cggatgcgat cgatcgtcgt ggcggcgcgt taggccgtga
     1021 ggctcgggac gagttgcgcg gaggctggga gctccgggta ttcgcgcagt aagcgcccat
     1081 atccttccac gcgatcgcga atgcccaacc gtcgcaggac ataccagagt gcaccctggt
     1141 tacttgtgac gaccggcacg ccgagatcct gctcgagcca gtcgatcact tcgatggtgc
     1201 gaacggctgt acagctcagg aacaccgcgt cggcttcggc ggtgtaatgc tcgcgcacga
     1261 tgcggtacca ctcgcccggt tcctgttggc gtcgttcgcg gagcgtgtcc agcgccacat
     1321 tggtcgtcac ttcgaagccg tgaccctcga gaaacgccac ctcgagcgca ttcgtcgatg
     1381 cggcgtacgg ggtaatgagc gcaatgcgtc gcgcgtcgag cgcgtgcaat gcctggacga
     1441 caccggacac ggtcgcacca cccgggtagc ccgtcgaccg ctcgatccgc gcggcgatct
     1501 gatcgtcaaa accgacaccg cccgcgacgc tcacggcggt gcagtgaaag aggatgaacg
     1561 atggacggag atcggccaga aggcccgcgg catcttcgat cccttcggcc atcttcaggt
     1621 agcggtccgc gccttcccgc tcgaggcgga gccgcgcggc aaagaactca accccgggcg
     1681 gtgccatcat ccgaaactgg ggctcggaca cggagttgcc cgatggcacg atgataccga
     1741 cctttgcgcg ccagccatat gtcatgatct gctcccttcg ggcgacctga atcgcggtac
     1801 gttcagcgga tggcacgcgt gccgcgaccg gcgccgcctg acaactggta cgggtcattc
     1861 cggacacgtc cgtccgtatg acgtggcgtg tgtccagcgg gaaggacgat gtggctcgtg
     1921 aacgccggtc cggatccgac taccatcctc gcctacaagt tggatcctca cgtcccgtcc
     1981 accattgcta catcgcatca tgccgacggt cacactacgg tttcgtattg atgatctggg
     2041 atgtacggtc cagccattcc tgcgattcca ccgtgcccag gatcgccttg tagtccttga
     2101 gctgcgacgg gtcgaacatg gcctttgggt ggtagaccgg cacgtcgagc cggcgcgtga
     2161 ccgcgatcgg gccgcctttc gacgcccacg catcctggcc ttcctggctc aggaaccagt
     2221 gcatgaacac gttcgcggcg ttcggatgcg gcgcgttttt gaaataagtg atgccggacg
     2281 ctcgtaagta ggtggtggcg acctcgcgga tctcctccac ttccaggccg atgcccttgg
     2341 ccttgtactc gtcgtaatcc gtctcgctcg cgcccagacc gatcggatac tggccggtgg
     2401 cgacccgctc ggcgagctgc cgctggtccg ccaatgcgac gaccgcctga tcgaagaaga
     2461 gcttcttgat gaagtcttcc cccttcacca tgctgaccgc agcgagggtg taggtggcgc
     2521 cgccgatacg ttccggggaa tacgtcacaa tcttgccctt gaacttcgga tcaaggatct
     2581 ggtcgatcga ggacagctcg gacgtcggga tcttcgatcg attgaccgag acgcccgttt
     2641 gcagctggat ctgcgtgatc aatgagtggg tatgctccgg accggcccac atctcgaatc
     2701 cgcccgccca cttgctatcg tcggtgatct ccttcggcag tcgggcgagc agcgggcgca
     2761 tatcctgcag cgcgtcagca ttaacgaact gcacgttcga gccgccgaat ccacccagga
     2821 tttcgacgtc ccagagatac tggccgttcc gctgctcggc caggacacgg ttggcgaact
     2881 ccccgcgcgg cgccgatgtg gcttccacct tcaaccacgg gtacttctct ttgaatgctc
     2941 cgagcacatc attctggagc agggacgaag gctgatggtt gatgctcaga acgccttcct
     3001 tcttcgcggc
//
LOCUS       DATAOZ010000578         6044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1539286, whole genome shotgun sequence.
ACCESSION   DATAOZ010000578 DATAOZ010000000
VERSION     DATAOZ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539286"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1286)
                     /locus_tag="VHR64_14560"
     CDS             complement(120..1286)
                     /locus_tag="VHR64_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX3304106.1"
                     /translation="MTQDTAEQAHPLTVLSEDEAILRDSVREFADAEVRPLVREMDEH
                     AKIPRTLLDRLFQLGVMAIEIPESYGGSGGTFFHAVLAVEELSRVDPSIGVLVDVQNT
                     LVINALLRWGSDDVKRRYLPRLASNVVGAYALSEAGSGSDAFALTTAARKDREGFVLN
                     GRKLWITNANEADLFIVFATIDPSAGYRGITAFLVERGFDGFVVGKKEDKLGIRASST
                     CELIFEDCYVHEGNILGEPGKGYKTAIETLNEGRIGIGAQMVGLAKGALQHAISYTKE
                     RKQFGKAIADFQSVQHQLARAAVDVEAATLAVYNAARLREAGRPFLTEAAICKILASE
                     VAERVASLAVNLLGGNGFVKEYPVEKLYRDAKIGQIYEGTSNLQLQTIAKQILG"
     gene            1391..3484
                     /locus_tag="VHR64_14565"
     CDS             1391..3484
                     /locus_tag="VHR64_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3304107.1"
                     /translation="MNTPARAISALFGLTLLCAAVALQGAASGQQSNGQSTPTQSGAG
                     QQNTTQQNSDQQPTVIRRGINYVSVDVIVTDKDGKPVLDLTQDDFSLAEDGKPQTIDN
                     FSVIKLDPVETAMTSPPREIRDDNDEQREAARPDVRLFVILLDDYHVQRGNDLAVRKP
                     LIDFVQNQLAPSDMVAIMYPLTSIDDLRFSRNRDATASAIDHFLGRKYDYVPRNEFEE
                     KYANYPAQVVERVRNQVTMDALKGAAMRMGALREGRKSIIFVSEGFTGILPAQMSDPI
                     ASMPGFNNPYRGSSSAPQATARDQMISDQDLLFDEQQVFTAMARMNTSIYPVDPRGLA
                     VFEYGVEKGIGITQDATSLRQMQDTLHVLADNTDGRAIVNRNDLANGMKQIIRDSSGY
                     YLLGYNSSQAPTDGKFHNIKVNVKRKGVEIRARKGYWAYTADDVARVNSAKTTEAPTA
                     VTTALNDLAAPTRDKSAHFWIGTSKGENGMTHVTFSWEPAPPIPGRPVEEPPARVALT
                     AVAQDGRPVFRGKVPDDAGSGSSAPSKGAAVSFDVPPGQLDFRMVVENTRGQVIDTLG
                     RQVTAPDFTNVSMSFGTPRIYRVRTIPELQALKNNPNAVPTTEREFSRTDRLFIRVDA
                     YAPGGVTPPVTARLLNRTGQSMADLPVKQSPGHPAEIELTLSAFAAGEYLVELNARSE
                     GGSSAQELVAFRIGR"
     gene            complement(3542..4792)
                     /locus_tag="VHR64_14570"
     CDS             complement(3542..4792)
                     /locus_tag="VHR64_14570"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX3304108.1"
                     /translation="MEAGGNIFNAMLQEFDGAARLLGLDPGIWKILTHPKRQIMVSLP
                     VQMDNGEIEVFTGYRVQYNITLGPAKGGIRYHPDVTLDEVTALAAWMTWKCAVAHVPF
                     GGGKGGVVCDPTRMSRRELEALTRRYVAEIVDAIGPEKDVPAPDVNTNDQIMAWVMDT
                     YSMHVGHTSTAVVTGKPIEMGGSLGRREATGRGVMIVTREAAKHLGFDIKGARVAVQG
                     FGNVGSVSADLLSRIGAKIVAVTDWKGGVYNADGLDIAKMLDYSKQHKTIDGFPGGEP
                     LENDQLFALDVEVLVPAALENQITMDNAPTIRARVVAEGANGPTTPEAHKHLHDRGIF
                     VIPDILANAGGVTTSYFEWVQDRHGYFWEEDEVNRRLEAKMVEAFDDVLKTSMKYKTD
                     LRTAAYIVAISRVATVTKMRGMYA"
BASE COUNT         1154 a         1884 c         1856 g         1150 t
ORIGIN      
        1 gtcgtagtcg aatccgtagt tcaacgtcgg gctatacagg agaaatgtcg agatcaatgc
       61 gaccagcacc agacatggtg cgagtgcgac ggggcgcacc tcaacgtcct gctgacgttt
      121 caaccaagga tctgcttggc aatggtttgc agctggagat tcgaggtgcc ttcgtagatt
      181 tgcccgatct tcgcgtcgcg gtagagtttt tcgaccggat attccttgac gaatccgttg
      241 ccgcctaaga ggttgaccgc cagcgatgcg acccgttcgg cgacttccga tgcgaggatc
      301 ttgcagatcg ccgcttcggt gaggaacggc cggccagctt ctcggagccg ggccgcgtta
      361 tagacggcca gcgtcgccgc ttcgacatcg accgccgcgc gcgccagctg atgctggacc
      421 gactgaaaat cggcgatcgc ctttccgaac tgtttgcgtt ccttcgtata gctaatcgcg
      481 tgctgaagcg cgcccttcgc cagtccgacc atttgcgcac cgatcccgat tcggccttcg
      541 ttcagcgtct cgatcgccgt cttgtagccc ttgcccggtt cgccgaggat gttcccttcg
      601 tggacatagc aatcctcgaa gatcagctcg caggtgctgc tggcccggat cccaagcttg
      661 tcttcctttt tgccgacgac gaacccgtcg aacccgcgct cgaccagaaa tgctgtgatc
      721 cctcgatagc cggctgacgg gtcgatcgtc gcaaacacaa tgaagaggtc ggcttcattc
      781 gcgttggtaa tccagagctt ccgcccgttg agaacgaatc cttcgcggtc tttccgtgcg
      841 gcagtcgtca gggcgaaggc atcgctgccc gaacccgcct ccgacagcgc ataggccccg
      901 accacgttcg acgccaaccg cggaagatag cggcgcttga cgtcgtcgct gccccatcgc
      961 aacagcgcgt tgatcaccag cgtgttctga acatcgacca gcacgccgat cgacggatcg
     1021 acacgcgaga gctcttcgac tgccagcacg gcgtgaaaaa acgttccgcc ggaaccgccg
     1081 tagctctcgg gaatctcgat ggccatgacg ccgagctgga acagccgatc gagcagcgtg
     1141 cgtgggatct tcgcgtgctc atccatctcc cgcacgagcg gacgaacttc cgcgtcggcg
     1201 aactcgcgaa cgctgtcgcg caggatcgct tcgtcctcgg acaagactgt caatggatgc
     1261 gcctgctcgg cggtgtcttg cgtcacgttc tctcctcgag cgcgggtcgt cggcagaagg
     1321 tcaatggtat cattgggtca cgagcccgcg cgtctcgaac cgcctgcccg aggctggagt
     1381 ctaaaccgtt atgaacaccc ctgcgcgtgc catttccgcc ctttttggcc tgacgctcct
     1441 ttgcgctgcc gtggctcttc agggtgccgc ctccggccag caatcgaacg gacaatcaac
     1501 cccgacccag tctggcgccg gccaacagaa caccactcag cagaattccg atcagcagcc
     1561 aacggtcatc cgccggggga tcaattacgt cagcgtcgat gtcatcgtca ctgataaaga
     1621 cggcaagccc gtcctggatt tgacccagga cgatttctcg ctcgcagaag acggcaagcc
     1681 gcaaacgatc gacaactttt ccgtgatcaa gctcgatccg gtcgagaccg cgatgacatc
     1741 gccgccgcgc gagattcgcg acgacaacga cgagcagcgc gaggcggcgc gcccggatgt
     1801 gcgcctgttc gtcattctgc tcgacgatta tcacgttcag cgtggtaacg accttgcggt
     1861 caggaaaccg ctgatcgact tcgtgcagaa ccagctcgcg ccgtccgaca tggtcgcgat
     1921 catgtatccg ctcacctcaa tcgacgatct gcgcttcagc cggaaccgcg atgcgaccgc
     1981 ctcggcaatc gatcacttcc tcggacgcaa gtacgactac gtgccgcgca acgaattcga
     2041 ggagaagtac gcgaactatc cggcacaggt ggtcgaacgc gtgcgcaatc aggtgacgat
     2101 ggacgccctg aagggggcgg cgatgcgaat gggggcgctc cgcgaggggc gcaagtcgat
     2161 cattttcgtc agcgaggggt tcaccgggat tctgccggcg cagatgtccg atccgatcgc
     2221 gtcgatgccc gggttcaaca atccgtatcg cggcagctcg agcgcgccgc aagcgacggc
     2281 ccgggatcag atgatctccg accaggattt gctgttcgat gagcagcagg tcttcaccgc
     2341 aatggcccgc atgaacacgt cgatctatcc ggtcgatccg cgcgggttgg cagtgttcga
     2401 atatggcgtc gaaaagggca tcgggatcac gcaagacgcg accagccttc gccagatgca
     2461 ggacacactc cacgtgctcg ccgacaatac cgacgggcgc gcaatcgtga accgtaatga
     2521 tcttgcgaac gggatgaagc aaatcatccg cgattcgagc ggttactacc tcctcggcta
     2581 taactcgtcg caggcgccta ccgacggcaa gttccacaac atcaaggtca acgtcaagcg
     2641 caagggcgtg gagattcggg cccgcaaagg gtattgggcg tacaccgccg acgatgtggc
     2701 gcgcgtcaat tctgccaaga cgaccgaggc gcccaccgcc gtcacaaccg cgctcaacga
     2761 tctcgccgcg ccgacgcgcg acaagtccgc ccacttctgg atcggaacct cgaaaggcga
     2821 gaacggcatg acccacgtga ccttctcgtg ggagccggcg ccgcccattc caggccgtcc
     2881 ggtcgaggag ccgccagctc gagtagcgtt gaccgcggtc gcgcaagacg gtcgtccggt
     2941 tttccggggc aaggttccag acgatgcggg ctcgggttcc agcgcgccgt cgaagggagc
     3001 ggccgtatcg ttcgatgtgc cgcccggcca gctcgatttc aggatggtgg tcgagaacac
     3061 tcgtggacag gtgatcgaca cgctgggacg acaggtcacg gcgccggatt tcaccaatgt
     3121 gtcgatgtcg ttcggaacgc cgcgcatcta ccgcgttcgc accataccgg aacttcaagc
     3181 gctgaaaaac aacccgaacg ccgtcccgac gaccgagcgc gagttcagcc gcaccgaccg
     3241 gctctttatc cgagtcgatg cctacgcacc gggcggcgtc acccctccgg tcaccgctcg
     3301 cttgttgaac cgcaccggcc agtccatggc cgacttgccg gtcaagcagt cacctggcca
     3361 cccggccgag atcgagctca cgctgtcggc ctttgccgct ggtgagtatc tggttgaatt
     3421 gaacgcgcga agcgaaggcg gcagctcggc gcaggaactg gtcgctttca ggataggacg
     3481 ctaaaaaggt aaaaggtaaa aggccttttt cttttacctt tttcctttta cctttttcct
     3541 ttcatgcata catgcccctc atcttcgtca cagtggctac ccggctgatg gcgacgatgt
     3601 aggcggcggt gcgcaagtcg gtcttgtatt tcatcgacgt cttcaacaca tcatcgaacg
     3661 cttcgaccat ctttgcttcg aggcggcgat tgacctcgtc ctcctcccag aagtatccgt
     3721 ggcgatcctg gacccattcg aagtatgagg tggtgacgcc gccggcattg gccaggatgt
     3781 cgggaatgac gaagatgccg cgatcgtgca ggtgcttgtg cgcctccggg gtggtcgggc
     3841 cgttggcgcc ttcagccacg acgcgcgcac ggattgtcgg cgcgttgtcc atcgtgattt
     3901 ggttttcgag cgcggctggc accaggactt cgacatcgag agcaaacaac tgatcgtttt
     3961 cgagcggctc gccgccgggg aatccatcga tggtcttgtg ctgcttcgag tagtcgagca
     4021 tcttggcgat gtcgagcccg tcagcgttgt agacgccgcc tttccagtcg gtgacggcca
     4081 cgatcttcgc gccgattctt gagagcagat cggcggagac cgatccgacg ttgccgaagc
     4141 cctgcacggc aacgcgcgcg cccttgatat cgaagccaag atgcttggcc gcttcccggg
     4201 tgacgatcat taccccgcgc ccggtggctt ctcggcgacc gagggaccca cccatctcga
     4261 tcggcttgcc ggtgacgacc gccgtcgagg tgtggccgac gtgcatgctg taggtgtcca
     4321 tcacccacgc catgatctga tcgttggtgt tgacgtcggg tgcgggaaca tccttttccg
     4381 gcccgatcgc gtcgacgatc tcggcaacgt agcgccgcgt gagggcttcg agctcgcgcc
     4441 gcgacatgcg tgtcggatcg cagacgacgc cccctttgcc gccgccaaac gggacgtgcg
     4501 ccacggcgca cttccacgtc atccatgcgg ccagcgcggt gacttcgtcg agcgttacgt
     4561 ccgggtgata gcggatgccc cccttggccg gaccgagtgt gatgttgtac tgaacacggt
     4621 agccggtgaa gacttcgatc tcgccgttgt ccatctgcac cggcagcgag accatgatct
     4681 gccgtttggg atgcgtgagg atcttccaga tccccggatc cagacctaac agccgcgcgg
     4741 ctccatcaaa ttcctggagc atcgcattga agatgttgcc acctgcttcc atgtgactcc
     4801 tgccacggct ccgggaaaac gactccacgc gatcccggtc cgctccaacg attgtcagtg
     4861 caaacaggtc aagctacccg tgcagacctc ggcaagtcaa ggaaacggcg ggtatgtctt
     4921 aattgtcata taaagtactt caattgacca tcaaaaccgt cgaccagcgg ttcaaaacgg
     4981 aacaataatc gggcaattac caatctggtt tgatgcagta aatcattgca gataaagtag
     5041 ttgatgttta ttgcaggggt ggtctccggc ttgctcaaaa catagcggtc cggagggact
     5101 ttatgcgctt agccacccgt acgttcgtgg tgtcatttct actggtgatg ctggggacgg
     5161 aattcgcgta cgcccagaaa aaagatacgg tcgttgccac cagctcaaat cagctcacga
     5221 tcctgagcac ggcggtcgat cgggtcaacc agacgctgac cgttcagggc atgggcttcg
     5281 gctcgcaagc accccaggtc tggtgcgaga catggccaat gacggtgatc agcgcaaccg
     5341 ataccgagtt ggtgattttt ctccccgcgg cagttccgga tggaagccac ctcctgacgg
     5401 tcgcccgcgg taacggcgac aaggatcggg gcgcgttcat catgtcagta ggcacggcgg
     5461 gacaagggac tgtcggcccg caggggccag ccggacccgt cggacctgct ggtcccgcgg
     5521 gaccgaaggg agatacaggc gcaaccggcg ccgccggccc agccgggccg aagggagatg
     5581 tgggtccggt cggacccgcc ggcccgaaag gggacacggg cgcggtggga ccggctggac
     5641 ccgctggtcc gcagggcgcg ataggtcctg ttggtcccgc cggacctgtg ggcgcaactg
     5701 gtgccgcagg accgcagggg gatgtgggtc cggtaggccc cgcaggacct gcaggagcga
     5761 agggtgatac cggtcctgcc ggcccggctg gaccggtcgg gcccaaggga gatgtcggcc
     5821 cggtcggacc gcagggtgaa gttggtccag ccggggcggc gggacccaag ggtgatgtcg
     5881 gtccggtcgg ccctcagggt gaggttgggc ctgccggagc tgccggacct cagggacctg
     5941 ccggcccgca aggtctgact ggcgcgacgg gagcgacagg tgcgcaagga ccgcaagggc
     6001 ctgtcggtcc gcaaggcccg ccgggactca gcgctcagca ggtg
//
LOCUS       DATAOZ010000579         4809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7061918, whole genome shotgun sequence.
ACCESSION   DATAOZ010000579 DATAOZ010000000
VERSION     DATAOZ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7061918"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            387..3053
                     /gene="alaS"
                     /locus_tag="VHR64_14575"
     CDS             387..3053
                     /gene="alaS"
                     /locus_tag="VHR64_14575"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX3304109.1"
                     /translation="MSSDAQSRPSHGHRYMTGAEVRAAFINFFVSKGHREVLSSSLVP
                     INDPTVLLTTAGMQQMTPYFLGLEAPPQNRLCSVQKCFRTVDIEEVGDESHLTFFFML
                     GNFSIGDYFKRESLAWSWEFLTEVMGLPAERFYPTVHPDDADAYAIWRDEIGVPEERI
                     GKLSDNWWGPVGNSGPNGPDSEIYFDLGPEFGDTGAGPGDGSRYLEVWNNVFMEFLQA
                     PDGSRTKLPKQHVDTGMGLERLAMIMQGAKSVYDIDLYQTIIQKVAAQVGVTYGVDDE
                     TDRSLRVIADHIRGSVFLIADGVLPGNEGRSYVLRRILRRAIKNGRQLGLTKPFLADL
                     AKVVIQQFSDNYPELRERRSQIEKVLTHEEQTFGRTLTTGISRFQALAESIAANGGSV
                     VPGDEVFRLYDTYGFPADLTRDLAEDAGLSIDTVGFEAAMQAQREASRAGGGFKDTAR
                     HRAEMYVQAAGRKTEFLGYDALTAEATVLALIGPDGPVEEADAGQAVEVILDRTPFYG
                     ESGGQIGDTGRIRTETGLIDIDGTVKPAPDLFVHRGVVAEGFIKTGESVTATVDGSRR
                     RAIQRNHTATHLLHKALRVVLGDETHQAGSLVAPDRLRFDFTSLESMSPQQVDRVAEI
                     VNAEILEDHPVTWQQRSMKEAIADGAMALFGEKYGDVVRVVSIESFSKELCGGTHVRH
                     TGEIGPFMIVSEGSVAAGVRRIEALTGSAATNRMLSQQRLLDRVSRDLRTTWHELPEA
                     IGQLQERTRSAERETATMRGKLAGSLVDDLLQSAEEVSGVPVIAERVAIQERGDLRQL
                     ADRLRDRQQSGVVVLGAVVDEKPALLAMVSADLVGRGLKAGDVIREIAQHVDGRGGGR
                     PDLAEAGGKNPAGLDKALAAVRGTVESALNGN"
     gene            complement(3059..3343)
                     /locus_tag="VHR64_14580"
     CDS             complement(3059..3343)
                     /locus_tag="VHR64_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304110.1"
                     /translation="MQRWEYLLVACVPHSMVMRPKYENGGLIRDWVEGNVADYCNDRG
                     SEGWELVSAVNDPLSSRGEVIDLIFKRPVERGAPTGAVRPDFSPLSTQTD"
     gene            3704..4537
                     /locus_tag="VHR64_14585"
     CDS             3704..4537
                     /locus_tag="VHR64_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304111.1"
                     /translation="MALAQDATPAAGSYPTVEITARNYAFNIPANIAGGWTQLTFHNE
                     GPADHHAMFIKLNDGTTFDDLKKASEAPDLGALFALGVSIGGAPTISAGQTASAILNF
                     DPGNYAVICAIPDENGKPHYQMGMLNSVEVTTDTTELAQPQSDASVELTEFMFTGLPA
                     TVTTGAHTWEVKDTGRQLHEFVVYQIVAPGMTFDQVKGILMASAATPTASPAAESSPP
                     AGGPPFIAVGGTGPMSPGYTNYAVLDLKAAEYFAICFVPDVATGAPHFALGMMMGFTV
                     S"
BASE COUNT          870 a         1532 c         1538 g          869 t
ORIGIN      
        1 tgggtaatct cggtcaccga gttcgtgtcg gtgtctacgg gaagatgcgg accatgctgc
       61 tcgatcgcgc cgatcggctg cagaatgact ggatcgtacg ctgccaactc ggcgatgtgg
      121 tctcgccgga gcttctgcca acgtatttca ctggtgtccg aagacgggag atccgccaca
      181 acctctcctc tcaacctgcg tgcgaacgct ggtgtgccac tcgctcaacg atctaccgcc
      241 acgaccattg tccccgactt ttcgccttct cgccgcgaaa ctgatcgcct ctctatactt
      301 aatgttcgcg acgcggcgga tgaaccagcc gcgttttgcg tatgtccgcg caggccgaga
      361 tcggcccaac cgaaggatcg tccgagatga gctccgacgc gcagtcccgt ccgtcccatg
      421 gtcaccgcta tatgaccggg gcagaggtgc gcgccgcttt catcaacttc ttcgtatcga
      481 aggggcatcg cgaggtgctc tcctcttccc tggtcccaat caacgaccca accgtgctgc
      541 tgacgaccgc cggcatgcag cagatgacgc cgtactttct cgggctggag gcgccgccac
      601 agaaccggct ctgctcggtc cagaagtgct tccgcacggt cgatatcgag gaggtcggcg
      661 acgagtcgca tctcaccttc ttcttcatgc tcggcaactt ctcgatcggc gactacttca
      721 agcgcgagtc gctggcctgg tcgtgggagt tcctgaccga ggtaatgggg ctgccggccg
      781 agcgcttcta cccgacggtc cacccggatg atgccgatgc ctacgctatc tggcgggacg
      841 agatcggcgt gcccgaggag cggatcggca agctctccga caactggtgg ggcccagtcg
      901 gtaactccgg tccgaatgga cccgactccg agatctactt tgacctgggg cccgagtttg
      961 gcgacaccgg cgccggtccg ggcgacggca gccgctacct cgaggtctgg aacaacgtct
     1021 tcatggagtt cctgcaagcg ccggacggct cccgcacgaa gctgccgaag cagcacgtcg
     1081 acaccggtat ggggctcgag cgccttgcca tgatcatgca gggcgcgaag tcggtctatg
     1141 acatcgacct gtatcagacg atcatccaaa aggttgcggc gcaggtcggc gtgacctacg
     1201 gcgtcgacga cgaaaccgat cggtcgttgc gggtcatcgc cgaccacatc cggggcagcg
     1261 tcttcctgat cgccgatggc gtcctgcccg ggaacgaggg ccgctcgtat gtcctgcgcc
     1321 ggatcctgcg ccgcgcgatc aagaacggtc gccagctcgg tctcaccaaa ccgtttctgg
     1381 ccgacctggc gaaagtagtc atccagcagt tctcggacaa ctacccggag ctacgggagc
     1441 ggcgctcgca gatcgaaaag gtcctcacac acgaggagca gacctttggg cgaacgctga
     1501 ccaccggcat cagtcgcttc caggctctgg ctgagtcgat cgcggcgaac ggcggatcgg
     1561 tcgtgccggg cgacgaggtc ttccgcctgt acgacacgta cggattcccg gctgatctga
     1621 ctcgcgacct cgcggaggac gccgggctga gcatcgatac ggtcgggttc gaggcggcga
     1681 tgcaggccca gcgcgaggcc agccgcgcgg gtggcggttt caaggacacg gcgcgacacc
     1741 gggctgagat gtatgtccag gcggccggcc ggaagaccga gttccttgga tacgacgcgc
     1801 tcacagccga ggcgacggtg ctggcactga tcggtccgga tggcccggtc gaggaggccg
     1861 atgccggtca ggccgtcgag gtgatcctgg atcgcacgcc attctatgga gaatccggcg
     1921 gccagatcgg cgatacgggc cggatccgca ccgaaaccgg gctgattgat atcgacggca
     1981 cggtcaagcc cgcgccggat ctcttcgtgc atcgcggcgt agttgcggaa ggattcatca
     2041 agaccggcga gtcagtgacg gcgacggtgg atggctcgcg gcgccgcgcg attcagcgta
     2101 atcacacggc cacgcaccta ctgcacaagg cgctgcgggt cgtactcggc gacgagaccc
     2161 accaggccgg ctcactggtc gcgccggatc ggttgcgctt cgacttcacc agcctcgagt
     2221 cgatgtcgcc gcagcaggtc gaccgcgtcg ccgagatcgt caatgccgag atcctcgagg
     2281 accaccccgt cacctggcag cagaggtcga tgaaggaagc gatcgcagac ggagcgatgg
     2341 cgctgttcgg cgagaagtac ggtgacgtcg tccgcgtcgt cagcattgag agcttctcga
     2401 aggagctctg cggcggcacg catgtccggc acaccggcga gatcggaccg ttcatgatcg
     2461 tgtccgaggg cagcgtcgcg gccggcgtgc gccggatcga ggcgctgacc ggctcggcgg
     2521 cgaccaatcg catgctctcg cagcaacgac tgctcgatcg cgtctcgcgc gacctgagaa
     2581 cgacctggca cgagctgcct gaggcgatcg ggcagcttca ggagcggacc cgttcggcgg
     2641 agcgggagac ggcgaccatg cgcggcaaac tggccggatc gctggttgat gacctgctgc
     2701 aatcggccga ggaggtttcc ggggtgccgg tgatcgccga gcgcgtcgcg atccaggagc
     2761 ggggcgacct gcgccagctc gccgatcgcc tacgggaccg ccagcagagc ggcgtagtcg
     2821 tgcttggtgc cgtggtcgac gaaaaaccgg cccttctggc gatggtgtcg gccgacctgg
     2881 tcgggcgcgg cctgaaggcg ggcgatgtga tccgcgagat agctcagcac gtcgatggac
     2941 gcggcggcgg gagacccgac cttgctgaag cgggcggcaa gaacccggcc ggtctggaca
     3001 aggcgctggc ggccgttcgc ggcaccgtgg aatccgccct caacggcaac tgagcgcgtt
     3061 agtccgtctg cgtcgacagg ggcgagaaat cagggcggac tgccccggtg ggtgctccgc
     3121 gttcgactgg tcgcttgaag atcaggtcga tgacctcccc tcgactggac aggggatcat
     3181 tgaccgcgct gacgagttcc cagccttcgc tgccgcgatc attgcagtaa tcggcgacgt
     3241 tgccttcgac ccaatcgcgg atgagccccc cattctcata tttcggacgc atgaccatcg
     3301 agtggggtac acaggccacc agcagatatt cccaacgctg catctgacct cctccctacc
     3361 aaccgggcca aaagtgtacg ttgcaaaccg gtgaactccc ggatacgact tctttgtcag
     3421 caatagatcc gaaattacgc acaattcgta tcggaatacg ataggaccgc ttgcgttgac
     3481 aaatcctgag tggatgccga cggttgcggg cggttctgcg agcgccgcgg gcggtccgcg
     3541 cgcttttcgg gattccctta atggaagcag gcaggtcgtc atgttcggaa ttcgttatcc
     3601 aggcgctggt cgggttggat cgacggcggt atcgcggcgt acgttgctgc acgcaggtgg
     3661 ggcactggca cttaccggtc tggtgggcca ttcgagtttg ggcgtggcgc tggcgcagga
     3721 tgccaccccg gcagcgggca gctatccgac cgtcgagatc actgccagga actacgcctt
     3781 caacattcca gccaatatcg ccgggggctg gacccaattg accttccaca acgaaggtcc
     3841 ggccgatcac cacgccatgt tcatcaagct caacgatggc acgacattcg acgatctcaa
     3901 gaaagcgtca gaagcacctg acctgggtgc gctcttcgct ctcggcgtca gcattggcgg
     3961 cgccccgacc atctcggccg ggcaaaccgc gtccgccatt ctcaatttcg atcctggcaa
     4021 ttacgcagtg atctgcgcaa ttccggatga gaacggcaag ccgcactacc agatgggtat
     4081 gttgaactcg gtggaggtga ccacggacac caccgagctt gcgcagccgc aatcggacgc
     4141 gtcggtcgag ttgaccgagt tcatgttcac gggactgcct gcaaccgtga cgaccggcgc
     4201 gcacacgtgg gaagtcaagg acaccggcag gcaactccat gagttcgtcg tgtaccagat
     4261 cgttgcgccg ggcatgacgt tcgaccaggt gaaggggatc ctgatggcct ccgccgcaac
     4321 tccgaccgcc tcgccggctg ccgagagctc cccgccggct ggtggtcctc cgttcatcgc
     4381 tgtcggcggc accggaccga tgagcccggg atacaccaac tacgcggtac tcgacctcaa
     4441 ggctgccgag tacttcgcga tctgcttcgt gccggatgta gcgaccggcg ctccgcactt
     4501 tgcgctcggc atgatgatgg gattcaccgt ctcctgacca atggaatatc cggcgagtca
     4561 tcggccataa ttggcccttt cgatgactcg ccggcacccg gttcgcccgc ctcccctgct
     4621 ctccgcctac aattccccct gcgccgttgc gggcgtctct ttttgtgacc tcgattggaa
     4681 tcaccaggca gcatgagtca acgtggatcg gggcggatcg aggtggggcc ggacgacgcg
     4741 ctccccgatc ttctcctgcg agtcaaggcc acccgcgggg atgaagccgt catcgtcatc
     4801 cccgacgac
//
LOCUS       DATAOZ010000580         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_750113, whole genome shotgun sequence.
ACCESSION   DATAOZ010000580 DATAOZ010000000
VERSION     DATAOZ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750113"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VHR64_14590"
     CDS             complement(<1..703)
                     /locus_tag="VHR64_14590"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX3304112.1"
                     /translation="MRAADYAYFDVPFAAYAHRGGATYPPNLHRENSLHAFQEAVALG
                     YRYLETDVHATRDGVLLAFHDGVLDRVTDRAGAIAQLSYAQVAEARIHGIDPIPRLSE
                     LLAEFPNARFNIDAKSPTAVALLASTIDEFEAHDRVCVSSFGVRRIHELRRRLGSRVP
                     SAASALGIAANRFLPWLTWVLNSPAPVLQMPISVPVAGRRLVVLTPALVEAVHRAGKR
                     VQIWTVDDSETMERLI"
     gene            complement(932..1423)
                     /locus_tag="VHR64_14595"
     CDS             complement(932..1423)
                     /locus_tag="VHR64_14595"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX3304113.1"
                     /translation="MVTLRRASETPHHPSSGPAGHQHDHHHHLLIEPEEDRWRWRRKI
                     RQDKRKLAVYRFVVGLFGLILIALGLVSGPLPGPGGIPLVLLGLAVWSSEFEWAHQLM
                     LWFKAQLYRYGSWSTPAKVLFWVVFFSCCGLLGYCYLLTLGIPGWLPTSVDVVLQRLP
                     GLS"
BASE COUNT          360 a          534 c          575 g          267 t
ORIGIN      
        1 cgataagcct ttccatggtt tcggagtcat cgacggtcca gatctgcacc cgcttgccgg
       61 ctcgatgaac tgcctcaacc aacgccggtg tcaaaacgac gaggcggcgt ccggccaccg
      121 gtacggagat tggcatctgc agcacgggcg ccggactgtt caaaacccag gtcagccagg
      181 gcagaaaccg attggcggca atacccaagg cacttgccgc ggacggcacc cgcgagccga
      241 ggcggcggcg cagttcgtgg atgcggcgga ccccgaacga gctcacgcat acccggtcgt
      301 gggcctcgaa ctcgtcgatg gtcgaagcga ggagcgccac ggccgttggt gatttggcgt
      361 caatgttgaa ccgcgcgttg ggaaactctg cgagcagctc ggacagtcgg ggtatcgggt
      421 caatgccgtg aatccgggct tcggcgacct gggcgtaact cagttgggca attgcgccgg
      481 cgcgatcggt cacccgatcg agtacgccat cgtggaaggc cagcagcacc ccgtcacggg
      541 tggcatgaac gtcggtctcc aagtagcggt aaccaagggc aaccgcctct tgaaacgcgt
      601 gcaggctgtt ctcgcgatgc aggttgggcg gataggttgc gccgccccga tgcgcgtagg
      661 cggcgaacgg gacgtcgaag tacgcgtagt cggcggccct catggggctc attgtgcctg
      721 cggatcgcag caacgaagcg cgatcccccg gccgcgatgt tcgcgccgcc gatctgctgg
      781 aggagccagc gaaacgtctc gagcagagcc gaatctcgag atgctcgttt cgtcggaccc
      841 gacgaagacc gcggcttccg agcgacatcg cagcgagccg cgcgagcaat ccaacacatc
      901 gagccttggc gaggggagca gtcaaacgcg gctacgacaa acccggcagg cgttgcagca
      961 ccacatcgac cgatgtgggc agccacccgg gaattcctaa cgtgagcagg tagcaatatc
     1021 cgaggagccc gcagcaggag aagaaaacca cccagaacag caccttcgcc ggcgtggacc
     1081 agctgccata gcgatacaac tgtgccttga accacagcat cagctggtga gcccattcga
     1141 actcacttga ccacaccgcc agtccgagca agaccagagg gatgccgccg ggtcccggga
     1201 ggggaccact gacgagacca agggcgatca ggataagccc gaagagtccg acgacgaacc
     1261 ggtacacggc cagcttgcgc ttgtcttgtc tgatcttgcg acgccaccgc cagcggtcct
     1321 cttcaggctc gatcaggagg tggtgatggt gatcgtgttg atgtccagcc ggtccagaac
     1381 tgggatggtg cggcgtctcg ctggcacgcc gcagcgtcac catgggaacc tcggcgtcgg
     1441 gggagtcttc gtcctcggcg ccgcgtgaca tggccctaac tctaagatga cgcgccaaaa
     1501 tgcgaggaat tggccggaga gcgatccgcg cccgcgatgt tcgcgccgcc gatcggcagg
     1561 aggagttggc ggcacacgct ttttcgttcc gtcggatccg acgaagacgg cggcagccca
     1621 gcgaacatcg cagcgagcgg gagcgagcaa tcgaacacag cgagcttggc gagcgaagca
     1681 atcagacacc ggatcgcgcg ccgctgtgct gaggaggagg gagcggacac gctctt
//
LOCUS       DATAOZ010000581         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7857460, whole genome shotgun sequence.
ACCESSION   DATAOZ010000581 DATAOZ010000000
VERSION     DATAOZ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7857460"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="VHR64_14600"
     CDS             <1..1326
                     /locus_tag="VHR64_14600"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX3304114.1"
                     /translation="NGINLIIGLPGSGKTILSQQFIFACATEKRPAIYLSTVSEPFEK
                     ILRYAQTLSFFDRGAIGRSVFYEDLGPTVAGEGGLTAVTEHVAALIKERRPGIIAIDS
                     FKALAAFADDAQGFRRFLHELAALLTAFPATSFWIGEYSEDETRAAPEFAVADGIIWL
                     ATERASERTLRLIQVSKLRGSDFRSGRHTYRLSQDGITVFPRLADPLSEADYRLSEER
                     ISSGIAPLDTMLAEGYAPGSSTLVAGPSGAGKTLMGLHFIFSGAANGEPGVIASLQEN
                     PVQLQRVARGFGWSLDDEHVAVMYRSPNDVYIDEWVYQLLDLIKTTGATRVMIDSLSD
                     LQYAAPDPVRFREFIYSLTQRLSRAGISPIMTSELPDLFHTGRLAEFGISHLSDNVIL
                     LQYLRADSRLLRTVTILKSRTSAHDPEIRKFDITSDGIVLGDPIGADHT"
BASE COUNT          275 a          556 c          445 g          252 t
ORIGIN      
        1 aacggcatca acctgatcat tgggctgccg gggtcaggaa agacgatcct gtcccagcag
       61 ttcatcttcg cttgcgcgac cgagaagcgc ccggccatct acctgtccac agtctccgag
      121 ccgtttgaga agatcctccg ctacgcgcag acgctcagct tcttcgaccg cggcgcgatc
      181 ggccggtcgg tcttctacga ggacctcggc cccacggtgg ccggcgaggg tgggctcacc
      241 gccgttaccg agcacgtcgc cgcgctgatc aaggagcgcc ggccgggaat catcgcgatt
      301 gacagcttca aggcgctggc cgcgttcgcc gatgacgcgc agggtttccg acgcttcctg
      361 catgagctcg ccgcactgct cactgccttc cccgccacca gcttctggat cggggaatac
      421 agcgaggacg agacgcgcgc ggctccggaa ttcgcggtgg ccgacggaat catctggctc
      481 gcaacagagc gcgcgagcga gcgcacgcta cgcctgatcc aggtcagcaa gttgcgtggc
      541 agcgactttc gctcaggacg ccacacgtat cggctctccc aggacggcat cacggtcttc
      601 ccccggctcg ccgatcctct cagcgaggct gactaccgac tcagcgaaga gcggatctcc
      661 tctggcatcg cgccgctcga cacgatgctc gccgagggct acgcaccggg tagctccacc
      721 ctcgtggccg ggccatcggg cgccggcaag accttgatgg gactccactt catcttcagc
      781 ggcgccgcga acggcgagcc cggggtgatc gcgagcctgc aagagaaccc ggtccaactg
      841 cagagggtcg cccgcggctt cggctggtcg ctggatgacg agcacgtggc ggtcatgtac
      901 cggtcgccca acgacgtcta catcgacgaa tgggtgtacc aacttctcga cctcatcaag
      961 accaccggcg ccacgcgggt catgatcgac agcctcagcg acctgcagta cgcggcccca
     1021 gatcccgtgc gcttccgcga gttcatctac tcgctcacgc aacgcctttc gcgtgccggc
     1081 atcagcccaa tcatgaccag cgaactccca gacctcttcc acaccggccg cctcgccgaa
     1141 ttcggaatct cgcacctctc cgacaacgtc atcctcctgc agtacctccg tgccgactca
     1201 cgcctcctgc gcaccgtgac gatcctcaag tcacgcacca gcgcccacga ccccgagatc
     1261 cgcaagttcg acatcacctc cgacggaata gtcctcggag atccgatcgg cgccgaccac
     1321 acctagcccg ggcgcacgag ctctcgctga gaatccagcc atctgccctg tccatgagtt
     1381 cttggccaat cgcgcgtagg gccggatggg ctcgggaagt gcgcccgcaa cggcaatgac
     1441 ttctacctcg agaagccggc gcgagcgcct gcgccgggcc tcgacggaac acgttccggc
     1501 ccgcgcgggc tttgaccgcg acgagtac
//
LOCUS       DATAOZ010000582         3204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_751222, whole genome shotgun sequence.
ACCESSION   DATAOZ010000582 DATAOZ010000000
VERSION     DATAOZ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751222"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VHR64_14605"
     CDS             <1..796
                     /locus_tag="VHR64_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3304115.1"
                     /translation="QICVENGRATGVVTTDGKEFHASVVLSNADPQRTYLKLVERDHL
                     PAEFLADIEAIQITSPVMKINLALDELPRYTAAGDNQSRAHTGGVFIGPSIDYMQRAF
                     EDARAGRPSDYPFFSVHTQSALDPSVAPEGKATLSIFTQYFPYELAEGTWGQRREEIA
                     DHAMRRYAEYAPNVPDAVVARQVLAPPDIEDRFGLTGGHIFQGELVPEQAFDMRPVPG
                     SKEYEGPIRGLYLCGSGAWPGGCVMGAPGHNAAHEAIGKLRAGRYR"
     gene            complement(804..2135)
                     /locus_tag="VHR64_14610"
     CDS             complement(804..2135)
                     /locus_tag="VHR64_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304116.1"
                     /translation="MFIRSRLIAVFAVLFLILTASGIAVAQTGQPDPNSPVKDRTPTP
                     EATESRSAPPKATAEATVRAVTPTPASSPEEGTPGAAATPLNGAEEIPLAAMTLDDTK
                     IPSDFQLIAETYTTPEELAAGMAGQISAEQIAATGMIGMYTSYYINRDRNTVRTYIIA
                     YKTVDGVQKGFDLLENEQLLVPNGSFTDKPGPTGVGEKPSEITSGFSENGDGTQTKSY
                     DISFRIDRYEVGAAMETTDESEPDTKVVDQMARDVADRVEAVLAGEQVSGVDLSLPGK
                     LLQLEQPTSLEGFETAAEAFAISNPNDAPAGYVAGYFKGVSYSTSVTTSLPVVTIGLL
                     TFESDNDVADALDNPESIMPSYTDLQELQRFAVNGADDAIAFSYSAPSGQGQPDSVRV
                     FAQVGDQMIVVDVQGMDTLKDATAAASDLTEAEIGCAVDGDCADPGAVSPQ"
     gene            complement(2209..>3204)
                     /locus_tag="VHR64_14615"
     CDS             complement(2209..>3204)
                     /locus_tag="VHR64_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX3304117.1"
                     /translation="MLDAERIVHLKPGAIVINAARGDVVDQDALAEAVASGHLRGAGV
                     DVFPFEPCTESPLFGLPNVVITPHTGGSSAEALANVGEMISTTTLAALAGQAVPNAVN
                     LPPASLLAPDLQRLTSVAAAAGHLLSVVQPELPSAFRVTIRGIVPADVVEHVTSNALA
                     EAINRWTGRWTTAVNARLVAEELGITLATLTGANDPDVEPSFSFEVLGETSHHVRVMW
                     DRRTAGIVEVDRFALDRALAGEILITHHRDVPGIVGRVGTIMGLYRVNIAGMQVGRHH
                     RGGEALMVLNVDDEIPQAALDEIKTIPGVETAYRVSLPPAQSRMATMTASASVAK"
BASE COUNT          543 a         1048 c         1033 g          580 t
ORIGIN      
        1 gcagatctgc gtcgagaacg gccgcgccac cggcgtcgtg acgacggacg ggaaggaatt
       61 ccatgcctcg gtcgtgctct cgaacgccga tccgcaacgg acctacctga aactagtcga
      121 gcgcgatcat cttccggctg agttcttggc cgacatcgaa gccatccaga tcacgtctcc
      181 agtgatgaag atcaacctgg cactcgacga gctgccccgg tacaccgcgg ccggcgacaa
      241 tcaatcgcgt gcccacaccg gcggcgtgtt catcgggccg agcatcgatt acatgcagcg
      301 cgcattcgag gacgccagag ccggacggcc gtctgattac cccttcttct cggtccacac
      361 ccagtcggcg ctcgatccct cggtcgcgcc ggaaggcaag gcgacgctct cgatcttcac
      421 gcagtacttc ccctacgagt tggcagaagg gacgtggggc cagcggcgcg aggagattgc
      481 cgatcatgcg atgcggcggt acgccgaata cgcgccgaac gtgccggacg ccgtcgtcgc
      541 ccggcaggtg ctggcgccgc cggatatcga ggatcgcttt gggttgaccg gcggccacat
      601 cttccagggc gagctcgtcc cggagcaggc tttcgacatg cgcccggtac cgggctcgaa
      661 ggagtacgag ggcccgattc gtgggctcta tctctgcggc tcaggcgcct ggccgggtgg
      721 ctgcgtcatg ggcgcacccg gtcacaacgc cgcgcacgag gcaatcggca agcttcgcgc
      781 cggccgctat cgataatccg gacctactga gggctcaccg ccccgggatc ggcgcagtcg
      841 ccgtccaccg cgcagccaat ctctgcctcg gtgaggtcag acgcggctgc agtcgcatcc
      901 ttcagagtgt ccatgccctg aacgtcgacc acgatcatct gatcgcctac ctgcgcaaac
      961 acgcggacgc tgtcgggctg gccctgccca ctcggcgccg agtagctgaa ggcgattgcg
     1021 tcgtccgcgc cgttgaccgc gaagcgctgg agttcctgga ggtcggtgta cgacggcatg
     1081 atcgactccg ggttatccag cgcatcagcc acgtcgttgt ccgactcaaa ggtcagcagg
     1141 ccaatcgtga caaccggcaa tgacgtcgtc acgctcgtgg aatacgagac gcccttgaag
     1201 taaccggcga cgtatccggc cggcgcgtca ttcggattgg agatcgcgaa cgcttccgca
     1261 gcggtctcga acccctcgag gctggtcggc tgctcgagct gcagcaattt gcccggcagg
     1321 ctgagatcga cgccggaaac ctgctcgcct gccagcaccg cctcgacccg atcggcgaca
     1381 tcgcgtgcca tctgatcaac aaccttcgtg tccggctcgg actcgtcggt cgtctccatc
     1441 gccgcgccaa cctcgtagcg gtcaatgcgg aaggagatgt cgtagctctt ggtctgcgtg
     1501 ccgtcgccat tctcggaaaa gccggatgtg atctcggacg gcttctcccc gactccggtc
     1561 ggaccgggtt tgtcggtgaa ggatccattc ggaaccagca gttgctcgtt ctcgaggagg
     1621 tcgaagccct tctgcacgcc gtcgacggtc ttgtacgcga tgatgtaggt gcgaactgtg
     1681 ttccgatccc ggttgatgta gtacgaggtg tacatgccaa tcatgccggt cgctgcgatc
     1741 tgctcggccg agatttggcc ggccatacct gcggccaact cctcgggagt cgtgtaggtc
     1801 tcggcgatca actggaaatc acttgggatc ttggtgtcgt cgagcgtcat tgccgcaagc
     1861 ggaatttcct ccgcgccatt cagcggagtg gcggctgcgc ccggcgttcc ctcctcgggc
     1921 gatgaggctg gcgtcggggt gacagcgcga acggtcgcct cggcggtcgc tttcggcgga
     1981 gcactgcgcg actccgtcgc ttcgggcgtc ggggtgcggt ccttgaccgg cgagttggga
     2041 tcaggctgac ccgtttgggc gacggcaatg ccggaggctg tgaggataag gaacaacacg
     2101 gcaaacacgg cgatcagccg ggaccggatg aacatgagca gcctcctgcg aaaccacgtg
     2161 tcgcctgtac cttactccat tgtcagccgc gcggattcac tgcgcccgtt atttggcgac
     2221 gctggccgat gcggtcatgg tcgccatccg gctctgtgcc ggcggcaatg aaacgcgata
     2281 ggcagtctcg acgcccggga ttgtcttgat ctcatcgagt gcggcctgcg ggatctcgtc
     2341 atcgacgttg agtaccatta gcgcctcgcc gccgcgatga tggcggccga cctgcatgcc
     2401 cgcgatattg acccggtaca accccatgat cgtgccaaca cggccgacga tgcccgggac
     2461 gtcgcggtgg tgcgtgatca ggatctcgcc ggccagcgcg cggtcgagcg cgaagcggtc
     2521 gacctcgacg attccggccg tgcgccgatc ccacattacc cggacgtggt gcgaggtctc
     2581 gcccagcacc tcgaacgaga acgacggctc gacatccgga tcgtttgctc ctgtcagcgt
     2641 cgccagcgtg atcccaagct cctcggcgac gagtcgggca ttgaccgccg tggtccagcg
     2701 gccggtccag cggttaatcg cttcggccag cgcgttgctg gtcacgtgct cgacgacatc
     2761 ggcagggacg atcccgcgga ttgtgacccg gaaggccgat ggcaactccg gctgcacgac
     2821 cgaaagcaga tgaccggccg ccgccgcgac cgatgtgagg cgctgcagat ccggcgcgag
     2881 cagcgatgcg ggcggcaagt tgaccgcgtt cggcaccgcc tgccctgcca gcgccgccag
     2941 cgtggtcgtg ctgatcattt cgccgacatt cgccagtgcc tcggcgctcg atccgccggt
     3001 gtgcggtgtg atcacgacat tcggcagtcc gaagagcgga ctctcggtgc agggctcgaa
     3061 tggaaagaca tcgacgcccg cgccgcgcag gtggccggat gccactgcct cggccagcgc
     3121 atcctgatcg accacatcgc cccgggccgc gttgatcacg attgcgcccg gcttgaggtg
     3181 gacgatccgt tcggcatcga gcat
//
LOCUS       DATAOZ010000583         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4434640, whole genome shotgun sequence.
ACCESSION   DATAOZ010000583 DATAOZ010000000
VERSION     DATAOZ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4434640"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..1416)
                     /locus_tag="VHR64_14620"
     CDS             complement(421..1416)
                     /locus_tag="VHR64_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HEX3304118.1"
                     /translation="MRALSRSDLRQVVPMHDAVELMKVAFRELSAGRALSPVRAQVPI
                     GDPNSLMLSMPGYVASEQAAGIKIVTFFGGNAGTDIPVIHALVYMIDADTGQPLGIME
                     GGYVTALRTGAVSGAATDLLARPDSSTLAVIGTGVQGVTQAAAVCCVRPITKIIGVDL
                     RPEAQQRFVDNVRRDWSELADCEITTTANADEAVASADVICCSTTSRQPVFSDSAVRP
                     GTHINAVGAFTPEMQEIPPATIARATVVVDNVDACLEEAGDLIKAMAEGAIDRDHWSR
                     ELGQIVSGDAPGRTTADEITFFKSVGNSVQDVVVGKRALSRADELKIGTELDLFA"
     gene            1683..>2100
                     /locus_tag="VHR64_14625"
     CDS             1683..>2100
                     /locus_tag="VHR64_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304119.1"
                     /translation="MTDPGLTRRLRQQGHRSGLAIGVSMALAIAILIGGFTWIYVQLD
                     PFVRDFAGAEPAPTQTPVEQAASSGKKATKTPTPEESASDEPTQEPDATETPKPDSTK
                     ITSVDENNGDFKPDYQVVSLEPVRLRSGPGVTFDIVV"
BASE COUNT          389 a          693 c          669 g          349 t
ORIGIN      
        1 cgaccgctcc ggcactcggt gcgcgcagtc caatggcgcc cagggccagt acgaacatgg
       61 caaggaaggt gagcgcgggg atgcgggatt gtcgcttcat catgtcctcc agaccgtctg
      121 aagctgcgta gcgggatgac acaatcaata tacgccgcaa ccctgcacat cggccctgtg
      181 cctgcccata tcgcgcggcc ggtaagtgac gtagcgcggg gttaccacgc tccgcatcga
      241 tgatcctcac gcgccgttga cggagccgaa ccgtgaccgg ggttggttcc cttcaacgaa
      301 tgagccgtcc tcgggtcccg ggggcatgtt cctctctccg gctctctcct caaggaaagg
      361 tggcatcccc gcgctacgtt gcaggtcggg aatccgcccc caagccgcga tgattgccgg
      421 tcaggcgaag aggtcgagct ccgtgccgat tttgagctca tcggctcggg agagggcccg
      481 cttgccgacg acaacgtcct ggaccgagtt gccgacgctt ttgaagaagg tgatctcgtc
      541 ggccgttgtc cggcccggcg catcgccgct gacgatttgc ccgagctcgc gcgaccagtg
      601 atcgcggtcg atcgcgccct cggccatagc cttgatcagg tcgccggcct cctccaggca
      661 ggcatcgacg ttgtcgacca cgaccgtcgc ccgcgcgatc gtggcgggcg gtatctcctg
      721 catctccggc gtgaaggcgc cgaccgcatt gatatgtgtt cccggacgga cggccgagtc
      781 gctgaagacc ggctgccggg aggtcgtcga acagcagatg acatctgccg atgcgaccgc
      841 ctcatcggca ttggcggtgg ttgtgatctc gcagtcggcc agctccgacc agtcgcggcg
      901 gacgttgtcg acgaaccgtt gctgggcctc tggccgcaga tcgacgccga tgatcttcgt
      961 gatcggccgc acgcagcaga ccgccgccgc ctgggtcacg ccctgcactc cggtcccgat
     1021 cacggcaagc gtcgatgagt ccggccgagc gagcagatcg gtggccgctc ccgagaccgc
     1081 gccggtccgc agcgcggtca cgtagccgcc ctccatgatc ccgagcggtt gcccggtgtc
     1141 ggcgtcgatc atgtagacca gcgcgtggat gacgggaata tcggtgccgg cgtttccgcc
     1201 gaagaaggtg acgatcttga tcccggccgc ctgctctgac gcgacataac ccggcatcga
     1261 gagcatcagc gagttcgggt cgccgatcgg cacctgcgct cgcaccggcg agagtgcccg
     1321 gccggccgag agctcccgga aggcaacctt catcagctcg accgcatcgt gcatcggaac
     1381 aacctggcgc agatcgctac gggacaaggc acgcatcgtc gggaaacctt tcgactggac
     1441 cggcagttca agtgagcaga ttctaccgaa ggcgcggagt cgcggaggcg cgaaggcgcg
     1501 gagttggaag gcgagctggc gcgcagtcgg aagaaggaaa acgaacgacg aaagacgaaa
     1561 gacgagaaac ccaagaccta agacgaaaga ccaacttcgc gcctccgcgc cttcccgtct
     1621 ccgcgactct gctacacttc cgccgctccg cgatgcaccc ttcattgctt acatgaggcc
     1681 caatgacaga ccccggactt actcgaagac tcaggcagca gggccatcgt tccggcctcg
     1741 cgatcggtgt ttcgatggcg ctcgcgatcg cgatcctgat cggcggtttc acctggatct
     1801 acgtccagct tgatcccttc gtgcgcgatt ttgccggggc tgagccggcg ccgacgcaga
     1861 ctcccgtcga gcaggcagcg agctccggca agaaggcaac caaaactccg acgcctgagg
     1921 aatcggccag cgatgagccg acgcaggagc cggacgcgac cgagacgccc aagcccgaca
     1981 gcacgaagat cacgtcggtt gacgaaaaca acggcgattt caagccggac taccaggtgg
     2041 tctcgctcga gccggttcgc ctgcgcagcg ggcccggcgt caccttcgat atcgtggtcg
//
LOCUS       DATAOZ010000584         3400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2859545, whole genome shotgun sequence.
ACCESSION   DATAOZ010000584 DATAOZ010000000
VERSION     DATAOZ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2859545"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..607)
                     /locus_tag="VHR64_14630"
     CDS             complement(8..607)
                     /locus_tag="VHR64_14630"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX3304120.1"
                     /translation="MSQSKLDRDDLHEIWATDRATWRAWLEEHHDREPGVWLFYWKKA
                     SGKAAMDWSDAVDEALCFGWIDSTRKTVDAHSFKQYFAPRKPKSNWSKINKEKIERLT
                     AEGRMAPAGFAAIELAKANGSWSNLDEVEAMVVPDDLIAAIDATAGARDYFDSLSRMK
                     LWEILYWINGAKREVTRADRIRQIAEAAAQGKRPDRFRP"
     gene            complement(604..1338)
                     /locus_tag="VHR64_14635"
     CDS             complement(604..1338)
                     /locus_tag="VHR64_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3304121.1"
                     /translation="MDDLSTLERVALRACPAAVECWLGQWLLRFGGGYTKRANSAYVM
                     GIEPDLDSSVRIEAAAAMMREQDLPLIIRESSLAVDPRISEALRQRGFHRIDETIVMT
                     AALPDPSGPEPEQVDLDTWLALYQRFEGGTRGSTAQYRTLLTRIEQPTCYGVLRSAGE
                     PVTIGQAVADGDWVGLYAIATDPARRRQGYGRSVVQRLLAWGSAQGATRSYLQVIATN
                     TPAVSLYEQLGYVESYRYWYWVQKSR"
     gene            complement(1346..2626)
                     /locus_tag="VHR64_14640"
     CDS             complement(1346..2626)
                     /locus_tag="VHR64_14640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3304122.1"
                     /translation="MRESSSEAPSQQAVSWRRNLYAITIAQWLAIVGFTLREPILPFY
                     LKHLGDLSTESATRWSGLFAAGGGLTMAITAPIWGLVGDKFGRKPMLLRSMLAASITV
                     GLMGMATAPWHIVGLRLVEGAFTGTVTASTALVATSAPRERLGFNLGMVQTAVFAGAA
                     FGPTIGGFSAAHFGYRATFAISAGLLISAVIMVIFFVREVFTKPEQPVGQAESFHAKW
                     AWMLGSVMLTMLAALFAVRFVQMGIRPVMPLYLQELGHYTDTHAATVSGWMFGVLGVS
                     SAASAIIFGRHGDKVGHQKILFWCVLGAGLLYLPMAIAQSAWHMIALQGLFGIAAGGM
                     IPSANAIIADVTPPDRRGFTYGITATAGAVGAAIGPLLLSTLIAPAFGFETAFVTVGI
                     LLIGLAAVLIVVTRTQRQPRHITESVFIAPGRGD"
     gene            2804..>3400
                     /locus_tag="VHR64_14645"
     CDS             2804..>3400
                     /locus_tag="VHR64_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304123.1"
                     /translation="MDGTRFDNFARSFASRRSRRGVLASAATVVAGAIGFHGRTEAQA
                     TGPAAGTVPCVNFLFGSGSATGGHHVTFRVRLSAPAPAGGANVTMGSSDAAITLPAAV
                     TVPAGATEYTFTATTHPVAADTVVVVSASTGACTVSRSVTIKAPVLRALHVQSVIRGG
                     GQGKITVCLNGATAAGGATVALSSSQPGVLPVPSSIVIP"
BASE COUNT          598 a         1114 c         1126 g          562 t
ORIGIN      
        1 agcgtggcta cggccgaaaa cggtccgggc gcttgccctg ggcggcggcc tcggcgatct
       61 ggcggatgcg gtcggcgcgg gtcacttcac gcttggcgcc gttgatccag tagaggattt
      121 cccagagctt catccggctc agcgagtcga aatagtcacg cgcgccggca gtagcgtcga
      181 tcgccgcaat caggtcatcc ggcacaacca tcgcctcgac ctcatcgaga ttcgaccagg
      241 agccattggc ctttgccagc tcgatcgcgg cgaacccagc cggcgccatc cgaccttcgg
      301 cggtcaaccg ctcgatcttc tccttgttga tcttcgacca gttgcttttc ggcttgcgcg
      361 gcgcgaagta ctgcttgaac gagtgcgcgt cgacggtctt gcgcgtgctg tcgatccagc
      421 cgaagcaaag agcctcatcg accgcgtcgc tccagtccat ggccgccttg ccgctggcct
      481 tcttccaata gaagagccag acacccggct cccggtcgtg atgctcctcg agccaggcgc
      541 gccaggtggc ccggtctgtc gcccatattt cgtggaggtc gtcgcgatcc agcttcgatt
      601 gactcatcgt gatttctgca cccaatacca gtaacgatat gactcgacat aaccgagctg
      661 ctcatagagt gaaacggccg gcgtgttcgt cgcgatgact tgcagatagg agcgggtcgc
      721 tccctgcgcg ctgccccagg cgagcagtcg ctggacgacc gaccgaccgt atccctgccg
      781 gcgacgggcg ggatcggtcg cgatagcgta aagcccgacc cagtcgccat cggcaacagc
      841 ctggccgatc gtcaccggct cgccggcaga ccgaagcaca ccgtagcagg tcggctgctc
      901 gatccgggtc aggagcgtcc ggtactgcgc agtgctgccc cgcgtcccgc cctcgaagcg
      961 ctggtagaga gcgagccacg tgtcgagatc gacctgctcc ggctccggac cgctgggatc
     1021 cggcagtgct gcggtcatga caatcgtctc gtcgatccga tggaagccac gctgccgcag
     1081 cgcctcactg attcgcggat cgaccgccag tgacgactcg cggatgatca ggggcaaatc
     1141 ctgctcgcgc atcatcgctg cggcggcctc gatccgcaca ctggaatcga ggtccggctc
     1201 aattcccatc acgtacgccg agttggcccg cttcgtatac ccgccaccga agcgcaggag
     1261 ccattgccca agccagcact ctacagcggc cgggcaggcg cgcaatgcga ctcgttccag
     1321 cgtcgagaga tcatccatca cctgactagt ctccccggcc aggtgcgatg aagaccgact
     1381 ccgtgatgtg ccgcggttga cgctgcgtgc gcgtcacgac gatgaggacc gcggcaagcc
     1441 cgatcaagag gatgccgacg gtcacgaagg cggtctcgaa gccgaacgcc ggcgcgatca
     1501 gggtcgagag gaggagtggg ccgatcgccg ccccgactgc tcccgcggtc gccgtgatgc
     1561 cgtaggtaaa gccgcgccgg tcgggcggcg tgacatcggc gatgatcgcg ttggcggagg
     1621 ggatcatccc gccggcggcg atcccgaaca gaccctggag cgcgatcatg tgccacgcgc
     1681 tctgggcgat cgccatcggc aggtacaaca ggccggcgcc gagcacgcac cagaagagga
     1741 tcttctggtg cccgaccttg tcgccgtggc ggccgaagat gatcgcggag gccgcgctcg
     1801 agacgccgag cacgccgaac atccagccgg agacggtcgc ggcgtgcgtg tcggtgtaat
     1861 ggccgagctc ttggaggtag agcggcatga ccgggcgaat gcccatctgc acgaagcgaa
     1921 ccgcaaagag cgcggccagc atcgtcagca tcaccgagcc gagcatccag gcccatttcg
     1981 cgtggaagct ctcggcctgg ccgaccggtt gctcgggctt ggtgaagacc tcgcgcacga
     2041 agaagatgac catgatcacc gccgaaatca gcagcccggc cgagatcgcg aaggtcgccc
     2101 ggtaaccgaa atgcgcggcc gagaagccgc cgattgtcgg tccgaaggcc gcgccggcaa
     2161 agacggcggt ctggaccatc cccaggttga agccaagccg ctcccgcggc gcgctggtcg
     2221 cgaccaaggc ggtcgaggcg gtgaccgtgc cggtgaaggc accctcgacc aggcgcaaac
     2281 cgacaatatg ccagggcgcg gtggccatgc ccatcaaccc gaccgtgatc gaggcggcga
     2341 gcatcgagcg gagcagcatc ggcttacggc cgaacttgtc gccgaccaga ccccagatcg
     2401 gcgcggtgat cgccatggtc aggccaccac cggccgcgaa gagtccggac cagcgcgtgg
     2461 cggattcagt cgagagatcg ccgaggtgct tcaggtagaa gggaaggatc ggctcgcgca
     2521 gggtaaagcc gacgatcgca agccactggg cgatcgtgat cgcatacagg ttgcgtctcc
     2581 aggaaactgc ttgctggctt ggggcttcgg aactcgattc acgcatagtg ctgcgacagt
     2641 ctaccgccga tccgaaaccg cgtgcctgca ctgggtgctt gtcgggagtg cgcccacgag
     2701 cgccattgac cgcagggtat cgcgttgata tggttcgggg gttttccggc ggaaaacagg
     2761 taacgaatgg cggttcggtg tcgagcagtg aggatttcac cggatggatg ggacgcgttt
     2821 tgacaacttc gcccgctcgt tcgcgtcccg tcggtcccgg cggggcgttt tagcgtccgc
     2881 ggcgacggtt gtcgcggggg cgatcggttt tcacggccgc accgaggccc aggcgaccgg
     2941 accggccgcc ggaacagtgc catgcgtcaa tttcctgttt ggctcgggca gcgcgaccgg
     3001 cgggcatcac gttaccttcc gcgtgcggct ctcggcccct gccccagccg gcggcgccaa
     3061 tgtgaccatg ggctcctcgg atgccgccat caccttgccg gcggcggtga cggtaccggc
     3121 gggcgcgacc gagtacacct ttacggcgac gacccatccg gtggctgctg atacggtggt
     3181 cgtcgtgtcc gcctcaaccg gggcatgcac ggtcagccga tccgtgacca tcaaggcgcc
     3241 ggtcctgcga gcgttgcacg tccagtctgt gatccggggc ggcggtcagg gcaagatcac
     3301 cgtctgcctg aacggggcga ccgcagccgg tggcgcgact gtcgcgctca gcagcagcca
     3361 accgggcgta ttgccggttc cgtcgtcgat cgtgatcccg
//
LOCUS       DATAOZ010000585         6248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6017413, whole genome shotgun sequence.
ACCESSION   DATAOZ010000585 DATAOZ010000000
VERSION     DATAOZ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6017413"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1060
                     /locus_tag="VHR64_14650"
     CDS             119..1060
                     /locus_tag="VHR64_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3304124.1"
                     /translation="MATVRAAQADSVPVVISRRKARWRRFPYLPYLLALPILAYEALF
                     ILYPIYQGVQSSFTKQTIGKPATWVGTANYRRLIHDDNFWHSVQQTTIYMVAVIVVAL
                     AFGLGSALLLNRPFHGRTIARATMTLPWAFPDVPTVLVFIWVLNPNFGVANRLTGTLP
                     FVSEHQKWLLDPNLAMPLVVLITAWKGFPFYSLVILAALQTVPQELTEAARVDGANKF
                     QAFWAVTLPCISPTLLLLTVLAAIFSFKQFTLVYLLTGGGPAGATDTLVVRIYQTAFR
                     FHNFPYAATIGVASFVMVITIALLFLAVQRRQEMAVS"
     gene            1132..1953
                     /locus_tag="VHR64_14655"
     CDS             1132..1953
                     /locus_tag="VHR64_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3304125.1"
                     /translation="MLYYLAVIIVVAIILFPVYWMVVSTLQPTKYSTYYPPPLFPKGA
                     NLTVFREVFDKYGVGGWILHSTILAFISTVIVLLLSIVGAYTLSSLRWRGRTVFAIFL
                     LMTQMLPEALIVVPIFKLFTNFPIIGVDLRNRLFGLSMLDAAFILPIGIWVLKNLYDT
                     IPREVQEAARVDGASQIRVLFQIIMPLTLPGLAAVGVIAFFYAWNEYLFAQLMISDND
                     LYPASVGLGFMKTMLDTPIELQLAAGLIFAIPPVFFYIVMQRYIVAGITAGAVKG"
     gene            1982..2965
                     /locus_tag="VHR64_14660"
     CDS             1982..2965
                     /locus_tag="VHR64_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX3304126.1"
                     /translation="MQPKVLIVYAGHKEVNVEREVLEAYGATVVAAVSPDTPEAEANY
                     TDCDALMVSIQPVTAEMMDRMPKCKIICRVGTGLDAIDIPAATERGIWVTNVPDYSID
                     EVSSHAMAFLLALARNLFRHRAMSQTGTWRYETERPIARIKGQTLGVLGLGRIGSASA
                     AKGQGLGLNVIACDPYLAEERFAELGVRRVDFETLLSESDFLTLHVPLTPETRHIINA
                     TALGKMKSTAFLINTARGEVVDIDALVDAVRAGRLAGAGIDVLPIEPPPPDHPILHED
                     SILVTPHIGWASTESGYDVRERGAQDVVLVLQGQRPKYPANEIAPVAATAD"
     gene            2977..4131
                     /locus_tag="VHR64_14665"
     CDS             2977..4131
                     /locus_tag="VHR64_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX3304127.1"
                     /translation="MSLEELQRAQSAIRDQGVDVALISSIANLTYVTGVEVALPVGAG
                     FEMAYAPWLAVVSGSDAGLVSGGEAGGMGVAGNGFELFTIEGFDSNQPTDPGGTYAMQ
                     LKAALSAAGGTGGTGTIGVESRSLPFVAGEIIRAALPNWTIVDIEPALIKARWNKTER
                     EIGLLRRASAVSDIAQRTLAELAHSAGQNEIEMFTEISRRAFEAAGGAIPLTGELVTG
                     PRTTVVAYPAGPKNRVTEPGDGALMDLSGRVNGYWFDCTNTHVIGGVEPTGEQRRYAR
                     ASQAACEAAMAALKPGNKASDASAAAAAAFASFGLPNAHYTGHQIGVTVNELPRLVPY
                     DHTVIEKGMVFSVEPGAYQGPGGTFGSRSEKMVLVREDGPEILSQFDWGI"
     gene            4133..5134
                     /locus_tag="VHR64_14670"
     CDS             4133..5134
                     /locus_tag="VHR64_14670"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX3304128.1"
                     /translation="MASTLDQTDTLSKEQILHLYREMVNIRRTEEQLAKSYQAGNIPG
                     ACHTYIGQEAVAVGVCTHLNHDDVVFSTHRGHGHALAKGVPPREVIAELFGKATGCSR
                     GRGGSMHLFSPEVGLMGTTGIVGPNILQATGAGYSFRLLKTDRVSVGFFGDGAVSNGA
                     FHEGINMAAIWDLPVIYVCENNMYATEVPFAYATKNQDVAERARSYGIPGIQVDGNDV
                     IAIYEAARAAVERARSGRGPTLIEARTYRTRPHAEGMRDAGYRTADEIQEWKQRDPIT
                     LLREGATARGVAADGDFDAIDEEIREVVADALQFATNSPFPEPSTATLFIYSQSQEA"
     gene            5143..6126
                     /locus_tag="VHR64_14675"
     CDS             5143..6126
                     /locus_tag="VHR64_14675"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX3304129.1"
                     /translation="MRELTFTAAAREGLAEEMARDETIFVVGEGIGARGGNFMTTAGL
                     YDVYGPERLRDTPIVERGFVGMCTGAAMTGTRPVVDFMFFDFVTDAFGEMINQTAKMQ
                     YMSSGRLKMPIVMRGAIGIGGSNATHHSGNYYPFFAHIPGWRVVVPTTPRCAKGLLKS
                     ALRSDDPVLFLEHKLLLNVKGEVPDPEADELIPFGKADIRRVGSDVTVVAIGAMVYKA
                     VEAAEQLTAEGISAEVIDLRSIAPLDLETVLASVHKTGRLVIVDEDFQPAGMGAEISA
                     LVTEHAFDDLDAPIRRVNGLHSPVPYSPSLEAAVIPNTSSIADAVRKLAAE"
BASE COUNT         1155 a         1943 c         1925 g         1225 t
ORIGIN      
        1 gctggtgcga tcaggcgatc gcaccagcga tctcatccgg tatgcgcccg tgctcgggtg
       61 cttggtttgt ttgccggggc ggtcggggat gaccggcccg gggtaaggtt tctcacccgt
      121 ggccacagta agggcagctc aggccgattc ggttccagtt gtgatatcgc gccggaaggc
      181 gcgctggcga cggtttcctt atctcccgta tctactcgcg ttgccgatcc tggcctacga
      241 agcgcttttc atcctctatc cgatctacca gggagtccag tcgagcttta ccaagcagac
      301 gatcggcaag ccggccacct gggtcggaac ggcgaactat cggcgcttga ttcatgacga
      361 caacttctgg cactcggtcc agcagacgac catttacatg gtcgccgtca tcgttgtggc
      421 gcttgccttc ggtctcggct cggcgctgct gctcaaccgc cctttccatg gccgcaccat
      481 tgcccgggcc acaatgacct taccctgggc gttccccgat gtgcccaccg ttttggtttt
      541 catttgggtt ctcaacccga acttcggcgt ggcgaatcga ctgaccggca ccttgccatt
      601 tgtcagcgag catcagaagt ggctgctcga tcccaatctg gcgatgccac tggtggttct
      661 cattacagcc tggaaggggt ttccgttcta cagcctggtc atcctggccg cgctccagac
      721 ggtgccgcaa gagctgacag aggccgcccg cgtcgacggc gcgaacaaat ttcaggcctt
      781 ttgggcggtc acgttgccat gcatttcgcc gacgctcttg ttgctgacgg tacttgccgc
      841 gatcttctct ttcaagcagt tcacactcgt gtacctgtta accggcggcg gacctgcggg
      901 cgcgaccgat accctcgtgg tccggatcta tcagactgca tttcgcttcc ataacttccc
      961 atacgccgcc accatcgggg tcgccagctt cgtcatggtc ataacgatcg ctctgctctt
     1021 cctcgcagtg caacggcgtc aagaaatggc ggtctcgtga tggctgtcgc ttcgccaccg
     1081 gccacgaaat ctgtaccgtc ccgcgtcgct ctgcggaagc ggaccggtac agtgctttac
     1141 tacctggccg tcatcatcgt cgtagcgatc atcctgttcc ccgtctactg gatggtcgtg
     1201 agcacgctcc agccgacgaa gtacagcacc tattacccgc cgccgctttt cccaaaggga
     1261 gccaacctca ccgttttccg tgaggtgttc gacaaatatg gcgttggagg ctggatcctg
     1321 cattcgacga ttctcgcctt catctcgacg gttatcgtcc tcttgctttc gatcgtgggc
     1381 gcctacacgc tctcgtcgct ccgctggagg ggccgaacgg tttttgcgat cttcctgctg
     1441 atgacgcaga tgctaccgga ggcgctgatc gtcgttccga ttttcaagct ctttaccaac
     1501 ttcccgatca tcggggttga cttgcgcaac cgtctcttcg gcctgtcgat gctcgatgcc
     1561 gcattcatcc tgccgatcgg catctgggtg ctcaagaatc tctatgacac gatcccccgc
     1621 gaagtgcagg aggctgcgcg cgttgacggc gcaagtcaaa tccgggtgct cttccaaatc
     1681 atcatgcccc tgacgcttcc ggggttggct gccgttggcg tcattgcatt tttctatgcc
     1741 tggaacgagt acctcttcgc gcagctcatg atttcggata acgacctcta tcctgcttcg
     1801 gtcggtctcg gatttatgaa gaccatgctg gacacgccga tcgagctgca actcgccgcg
     1861 ggtttgatct tcgccattcc gccagtcttc ttctatatcg tcatgcagcg atacatcgtc
     1921 gccggaatca ctgccggcgc cgtcaagggt taatcagtcg aggaaaggct ggggagtctc
     1981 ggtgcaaccg aaagtgctga tcgtgtatgc ggggcacaag gaagtcaatg tcgagcgtga
     2041 ggttctcgaa gcgtacggcg cgacggtcgt cgcggccgtc agccccgaca cgcctgaggc
     2101 tgaggcgaat tacacagact gtgacgcgct gatggtctcc attcaaccgg tcacggccga
     2161 gatgatggac cgcatgccaa agtgcaagat catctgccgg gtcggcaccg gtctcgacgc
     2221 gatcgacatc ccagcagcga ctgagcgggg aatctgggtc acgaacgtgc ccgactactc
     2281 gatcgacgag gtctcgagcc acgccatggc cttcctgctt gcgctagcgc gcaacctgtt
     2341 ccgccaccgc gcgatgtctc agaccggcac gtggcggtat gaaaccgagc gaccgatagc
     2401 gcggatcaag ggccagacac tcggcgtcct cggactgggt cggatcggca gcgcctcggc
     2461 cgccaaagga cagggactcg gtctgaacgt gatcgcgtgc gacccgtacc tggcggaaga
     2521 gcgatttgct gagctcggcg tccgccgcgt cgacttcgaa acactgcttt cggagtcgga
     2581 tttcctgacg cttcacgtcc cgctgacgcc cgagaccagg cacatcatca atgccaccgc
     2641 gctcggcaag atgaagtcga ccgccttcct gatcaatacc gcgcgcggcg aggttgtgga
     2701 tatcgatgcg ctggtggacg cggtccgcgc cgggcgactg gccggtgctg ggatcgatgt
     2761 ccttccgatt gagcctccgc cgcctgatca cccgatcctg cacgaggact cgatcctcgt
     2821 cacaccgcac atcggctggg cctcgaccga atccggctac gacgtgcggg aacgcggggc
     2881 gcaggacgtc gtgctggtgc tgcagggtca acgcccgaaa tacccggcca acgagatcgc
     2941 gccggttgcg gcaacggcgg attagggaca ccaaccatgt cgctggagga actgcaacga
     3001 gcgcaatcag cgatccgtga tcagggtgtg gatgtcgccc tgatctcctc gattgcgaac
     3061 ctgacctacg tgaccggagt cgaggtcgcg ctcccggtcg gcgcgggctt cgagatggcc
     3121 tatgcgccat ggctggctgt cgtatccggc tccgacgccg ggctcgtctc gggtggcgag
     3181 gcgggcggga tgggcgtagc cggcaacggg ttcgagctct tcacgattga aggcttcgac
     3241 agcaaccagc cgacggaccc gggcggcaca tacgccatgc aactgaaagc ggccctgtcc
     3301 gcagcgggcg gcaccggcgg cacgggcaca attggtgtcg agtcgcggtc actccccttc
     3361 gtcgccggcg agatcatccg ggcggccctg ccgaactgga cgatcgtcga catcgagccg
     3421 gcactgatca aggcgcgctg gaacaagacc gagcgcgaga tcgggttgct tcgaagggcg
     3481 tcggccgtct ccgacattgc ccagcgaacc cttgctgagt tagcgcacag cgcaggtcag
     3541 aacgagattg agatgtttac cgagatcagc cgtcgagcgt tcgaagcggc tggcggcgcg
     3601 attccgctca ccggcgagct cgtgacgggg ccgcgcacga ccgttgtagc gtatcccgcc
     3661 ggcccaaaga accgcgtcac cgagccgggc gacggcgccc taatggatct gagcggccga
     3721 gtcaatggct actggttcga ttgcacgaac acgcatgtga tcggcggagt tgagccgacc
     3781 ggcgaacagc gccgctatgc gagggccagc caagctgctt gcgaagccgc gatggccgcg
     3841 ctcaaacctg gcaacaaggc gagtgacgcc tctgctgccg cagcagccgc gttcgcctca
     3901 ttcggcttgc cgaacgccca ttacaccggt caccagatcg gtgtcacggt caacgagctg
     3961 ccgcgccttg tgccctatga ccacacggtg atcgagaagg gaatggtttt cagcgtcgag
     4021 ccgggtgcgt atcaaggacc aggcggcacg tttggatcgc gttcggaaaa gatggtcctc
     4081 gttcgcgagg acgggccgga gattttgtcg caatttgatt ggggtatcta gcatggcgtc
     4141 cacgctggac cagactgaca cgctttcgaa agagcagatc ctccatctct atcgggagat
     4201 ggtcaacatc cgccggactg aggagcagct cgcgaagagc taccaggcag gcaatattcc
     4261 cggcgcgtgc cacacctaca ttggccagga agcggtcgca gttggcgtat gtacacatct
     4321 gaaccatgac gatgtcgtct tcagcacgca ccgcggccac ggtcacgcgc tggccaaagg
     4381 cgtcccgccg cgagaagtga tcgctgagct tttcggcaag gcgaccggct gctcacgggg
     4441 gcgaggtggc tcgatgcacc tcttctcacc tgaggtcgga ctaatgggta cgaccgggat
     4501 cgtggggccg aacatcctcc aggcaactgg agccggctat agcttccgcc tgctcaagac
     4561 cgaccgggtc agcgttggct tcttcggcga tggagcggtc agcaacggcg cgttccacga
     4621 aggcatcaac atggcggcga tttgggatct gccggtcatc tatgtttgcg aaaacaacat
     4681 gtacgcgacc gaggtcccat tcgcctacgc gacgaagaac caggatgtcg ctgagcgcgc
     4741 tcgcagttat ggcatcccgg ggatccaagt cgatggcaac gacgtgatcg cgatctacga
     4801 agcagcgcga gcggcggtcg agcgggcgcg ctccggtcgc ggtccgactc tgatcgaggc
     4861 tcgcacctat cggactcggc cgcacgccga gggaatgcgc gacgcgggtt atcgaacggc
     4921 cgacgaaatc caggagtgga agcaacgcga cccgatcacg ctgcttcgtg aaggcgcaac
     4981 tgcacgcgga gtagccgctg acggagactt cgacgccatc gacgaggaga tccgggaagt
     5041 cgtggctgat gccctccagt tcgcgaccaa tagccccttc cctgaaccat cgaccgcgac
     5101 gttgttcatt tacagccagt cccaggaggc gtgaggcacc cgatgcgtga attgaccttt
     5161 accgccgccg cccgtgaggg cctggccgaa gagatggccc gcgacgagac aatcttcgtc
     5221 gtcggcgaag ggatcggtgc ccgtggtggc aacttcatga cgactgctgg tctctatgac
     5281 gtgtacggtc ccgagcggct gcgtgacacg ccgatcgtcg aacgcggctt cgtcggaatg
     5341 tgcaccggcg cggcaatgac cgggacgcgc ccggtggtcg acttcatgtt cttcgacttc
     5401 gtgacagatg cgttcggcga gatgatcaac cagactgcga agatgcagta catgagcagt
     5461 ggccggctca aaatgccgat cgtgatgcgc ggcgcgattg ggatcggtgg gtccaacgcg
     5521 actcaccact ctggcaacta ctaccccttc ttcgcccaca tccccggctg gcgcgtcgtt
     5581 gtcccaacga cgccccgctg cgccaaagga ctgttgaaga gcgccctgcg aagcgacgac
     5641 ccggtcctct tcctcgagca caaattgctg ctcaacgtga agggcgaggt cccggatccc
     5701 gaggcagacg agctcatccc gttcggaaag gccgacatcc gccgcgtagg cagcgatgtt
     5761 accgtcgttg cgatcggggc gatggtctac aaggcggttg aagctgccga gcaattgact
     5821 gcagagggaa tctctgccga ggtaattgac cttcgctcta tcgcgccact cgaccttgag
     5881 acggtcctgg cctcagttca caagaccgga cgcctggtca tcgtcgacga ggatttccag
     5941 cccgcgggaa tgggtgcaga gatctcggcg ctggtcaccg agcacgcctt cgacgacctg
     6001 gatgcgccga tccgccgtgt caacggactg cacagtcccg tgccgtacag cccgtcactg
     6061 gaggctgccg tcatcccgaa cacgagctcg atcgccgacg cggttcgcaa gctggcggcg
     6121 gagtaggcag tatggcgacc gaagttgtga tgcccagact cggctggacc atggaggtcg
     6181 gccgggtcgt cgaatggctc aagaatgatg gcgacacgat cgaagagggc gatctgatcc
     6241 tcgcgatc
//
LOCUS       DATAOZ010000586         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2597870, whole genome shotgun sequence.
ACCESSION   DATAOZ010000586 DATAOZ010000000
VERSION     DATAOZ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2597870"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VHR64_14680"
     CDS             complement(<1..188)
                     /locus_tag="VHR64_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304130.1"
                     /translation="MRADMVGGSEWRRRMLPGQLVDAECVPEVDRRDEPGMRIDPMEL
                     TSLDREEDDRGAILRAAD"
     gene            complement(198..1517)
                     /locus_tag="VHR64_14685"
     CDS             complement(198..1517)
                     /locus_tag="VHR64_14685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEX3304131.1"
                     /translation="MHSFPTIAHTWKAPQQKFLDHNWQDKNAITRIDQLEAKLAGWVR
                     PNVIKNIQEGLTKVGMSIRLTPYVMDLIDWDNAEADPIRRQFLPMLSELEADHPCLTV
                     DSLEERATSPAPNLVHRYPDKVLFLVTAMCPVYCQYCTRSYAVGQDTSILKKDSVAAS
                     GWIEALDYIRANPTVEDVVVSGGDIARLKAKNIALLGNALIDIEHVKRIRLATKAISV
                     QPMKFLSDDEWFKAITDVAARARERFKSVFLHTHFNHPREVTPLVEAAMRRVFSEGIY
                     VRNQSVLLRGVNDNSCTLVQLVKDLGRVNIQSYYVYACDMVVGCEHFRVPVQEMQHLE
                     REVRGVTAGFNTPLFVVDAPGGGGKRDVHSYEYRDDKYGIIGFRAPAVNPNRMYFYFD
                     PLRSLGEVAQTEWRMGGRDAILARLPHFGDRGRSLGQRGAMSAHESN"
     gene            1784..2383
                     /locus_tag="VHR64_14690"
     CDS             1784..2383
                     /locus_tag="VHR64_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304132.1"
                     /translation="MDLTDAQSERLFQAYEIAKGLLRELKIFPGEGRERDILDIDEFD
                     RSWPTMKLDDLRYLVSAIISIGEKSGEGEPPFRESQFYGKWNTARRRILPHFNSGDSD
                     AEAKKTKINSPIGWKALMAKLSRLQRLRIFDRGRDRAIEYDDEGAVAVAELLLAHGAD
                     ASVRNTAGLTPLKSPRRAVSRRRPTCSIPVRGREDGRTT"
     gene            complement(2672..>3571)
                     /locus_tag="VHR64_14695"
     CDS             complement(2672..>3571)
                     /locus_tag="VHR64_14695"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX3304133.1"
                     /translation="TALDEQGIAERYDIVVDFSIFPVNSRIKLVNLLKMRDDGRGPQG
                     ALSLAEALAGLLSDPVVGPMMEFRVVNSVPSVDQPGTTLTVANSCGPNDRSQVPAVLT
                     EQIPIVAPVRTRTVAFGRSGPGDSRDPVTGQCTPDCPEGMPFPWTIEVNGGPAHSFNA
                     NRISELIPKPGEIEHWTLVNKGGGWDHPIHLHFEEGITMNRGADPIPATELLVRKDVW
                     RLRDSGQVTFQVQFGEYGGSYVNHCHNTVHEDFALLMRIQLLAAAGPQVAVTPTPNPT
                     PDGVFFTTPEILPEGDPRGGATG"
BASE COUNT          669 a         1094 c         1107 g          701 t
ORIGIN      
        1 tcgtcggcgg ctcggaggat ggcgccccta tcatcttctt cccggtcgag gcttgtgagc
       61 tccatcgggt caatcctcat gcccggctca tcgcgccgat cgacctcggg tacgcattca
      121 gcatccacga gttgccctgg cagcatccgc cggcgccact ccgaccctcc gaccatgtcg
      181 gcgcgcatcg gttgtcctca gttggactcg tgcgcactca tcgctcctcg ctgacccagg
      241 gagcggccgc gatcaccaaa gtggggcagg cgggctagaa ttgcgtcgcg acctcccatg
      301 cgccattctg tctgggcgac ttcgccaagc gagcgcaaag gatcgaagta gaagtacatc
      361 cggttcggat ttactgccgg tgcgcgaaaa cctattattc catatttgtc gtcacggtac
      421 tcgtagctgt gcacatcgcg ctttccgccc cctccgggcg catcgacgac aaagagcgga
      481 gtattgaagc ctgccgtgac tccccgtacc tctcgctcga gatgctgcat ctcctggacc
      541 ggtactctga aatgctcgca accgaccacc atatcgcagg catacacgta ataggattgg
      601 atgtttacgc gaccaaggtc tttcacaagc tgcacgagtg tacaggagtt atcgttgacg
      661 ccccgcagaa ggacactctg gtttctgacg tatatgcctt ctgagaatac tcgacgcatt
      721 gcggcttcga ccaacggagt cacctcgcgc ggatggttga aatgggtatg taagaagacg
      781 ctcttgaacc gctctcgggc acgtgcggcg acatcggtaa tagctttgaa ccattcatca
      841 tctgaaagaa acttcatcgg ctgcacagaa atagctttcg tagcgagtcg gatccgctta
      901 acgtgctcta tatcgatcaa agcgttgccc aggagcgcaa tatttttcgc tttgagcctg
      961 gcgatgtctc cacccgagac aaccacatcc tctactgtag gatttgcgcg gatgtaatcc
     1021 agcgcttcta tccaacccga tgctgccacg ctatcttttt ttagtattga cgtgtcctga
     1081 cccactgcgt agctgcgtgt gcaatactga cagtaaacgg ggcacatcgc ggtcacgaga
     1141 aatagcacct tgtccggata tcgatggacg agattgggtg ctggcgaggt cgctcgctcc
     1201 tccagggaat ccaccgtaag gcaaggatga tcggcttcca gctcggaaag catcggtagg
     1261 aattggcgcc gaatggggtc tgcctcagca ttgtcccaat cgatcaagtc cattacatat
     1321 ggagtgagcc tgatcgacat gccgactttg gttaagcctt cttgaatatt tttgatcacg
     1381 ttgggcctta cccaaccagc caacttggct tccagctgat cgatgcgagt tatggcgttt
     1441 ttatcttgcc agttatggtc aagaaatttc tgctgagggg ctttccaggt gtgggcgatg
     1501 gttggaaacg agtgcatgag gcgtgctccg gccagacgga gagtctagac aaagaaacat
     1561 acattcactg cagagcgtgg ctacattccc ataggttttg aattaacgaa taacgttatg
     1621 actttattgc gctgctatgg tcaccaccgc ccctcggcgt ccgttccaac gcgcgtgacg
     1681 cattgagcga ggagcggact gattcgatct ccggatgtgt tgggcccact cacgaccagc
     1741 gaccgatcaa accgggcgtg gcccggtcgc cttcggcgag atcatggacc tgaccgacgc
     1801 ccagagcgag cgcctgttcc aggcttacga gatcgccaag ggcctgttgc gcgagctgaa
     1861 aatctttccc ggcgagggcc gcgagcgcga catcctcgac atcgacgagt tcgaccgcag
     1921 ctggcccacg atgaagctcg atgacctgcg ctaccttgtc tcggcgatca tctccatcgg
     1981 ggagaagtcc ggcgagggag agccgccctt ccgcgagtcc caattctacg gcaagtggaa
     2041 taccgcgcgg cggcgcatcc tcccacactt caacagcggg gattccgatg ccgaggccaa
     2101 gaaaaccaag atcaacagcc cgatcggctg gaaggccctg atggcgaagc tgagccgcct
     2161 gcaacggctg cgcatcttcg atcgtgggcg cgaccgcgcc atcgaatacg acgacgaggg
     2221 cgcggtcgcg gtcgcagagc tgctcctcgc tcacggcgcc gatgcatcgg tgcggaacac
     2281 cgcggggctc accccgctca agtcgcctcg ccgcgcggtc tcgaggaggc ggccgacttg
     2341 ctcaatccca gtcagaggcc gtgaagacgg caggaccaca taagccgaac tcggattatg
     2401 cgagaccgag agccagcaga tctgcagccg tgcagctcct gctcggtgat tcgaagatcg
     2461 acagcttccg ggtgttacgc aagcttggca tgctgcggtc tcccgcgccg gttctcttga
     2521 cggcgaagac gccgccacat gtgaaggttt cggacgccgg ccattgcgag ggtgaggcaa
     2581 gcgccggcct gcggcaggcc gaaggggcgc cctgggcgcg ccttcggtgg tcgtgcaacg
     2641 ccgcccggcc cctgagcgac ctgagcgggg gctagccggt ggcgccgcct ctggggtcgc
     2701 cctcgggcag gatttccggg gtggtgaaga ataccccgtc cggggtcggg ttgggggtgg
     2761 gcgtcaccgc cacctgcggc ccggccgcgg cgagcagctg gatgcgcatc aggagcgcga
     2821 agtcctcgtg cacggtgttg tggcagtggt tgacgtagga gccgccgtac tcgccgaact
     2881 gcacctggaa cgtcacctgc ccgctgtcgc gcagccgcca cacgtccttg cgcaccaaca
     2941 gctcggtggc ggggatgggg tcggcgccgc ggttcatggt gatgccttcc tcgaagtgca
     3001 ggtggatcgg gtggtcccag ccgccgccct tgttgacgag cgtccagtgc tcgatctcgc
     3061 cgggcttggg gatcagctcg gagatgcggt tggcgttgaa tgagtgggcc ggcccgccgt
     3121 tgacctcgat ggtccacggg aacggcatgc cctccgggca gtcgggcgtg cactgacccg
     3181 tgacgggatc gcgcgagtcg ccgggaccgg agcggccgaa cgcgaccgtg cgggtgcgca
     3241 ccggcgccac gatcgggatc tgctcggtca gcaccgccgg cacctggctc ctgtcgttgg
     3301 gaccgcagct gttggccacg gtgagcgtgg tgcccggctg atcgacgctc ggcaccgagt
     3361 tgacgacccg gaactccatc atcggcccga ccaccggatc gctcagcagg cccgccagcg
     3421 cctcggccag cgacagcgcc ccctgcggcc cgcggccgtc gtcgcgcatc ttgaggagat
     3481 tgacgagctt gatgcgggaa ttgaccggga agatcgagaa gtcgacgacg atgtcgtagc
     3541 gctcggcgat gccctgctcg tcgagcgcgg t
//
LOCUS       DATAOZ010000587         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6018084, whole genome shotgun sequence.
ACCESSION   DATAOZ010000587 DATAOZ010000000
VERSION     DATAOZ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6018084"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..426
                     /locus_tag="VHR64_14700"
                     /pseudo
     CDS             1..426
                     /locus_tag="VHR64_14700"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
     gene            430..870
                     /locus_tag="VHR64_14705"
     CDS             430..870
                     /locus_tag="VHR64_14705"
                     /inference="COORDINATES: protein motif:HMM:NF013399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HEX3304134.1"
                     /translation="MYGKATGNTPDGRRRGEPFAPGANPMHGRDSHGIHASAASVAKI
                     PYRDAADGISLTTTLVPSGLGRTAEDRITNLTGILDAFFSATGYHMNINVLNRDTLVD
                     AMEHPEKYPSLTIRVSGYAVNFVRLTREQQMDVINRTFHGEASV"
     gene            863..1699
                     /gene="pflA"
                     /locus_tag="VHR64_14710"
     CDS             863..1699
                     /gene="pflA"
                     /locus_tag="VHR64_14710"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HEX3304135.1"
                     /translation="MSEPAAVPLEAKSPYELRVHLGEGVPEQSVRDALATGDMGFLHS
                     YTTGSAVDGPGLRVVAWTTGCMWRCQYCHNPDTWTLTNGLPVSVSNAAAELLKYRQGL
                     KVMKGGFTLSGGDPLMQHRFAAKLLAAVKQMGIHTAIETNGFYGDRLSDAELDTIDLV
                     MLGIKSWDPARHLALTGKPIEPTLEFARRLAARKRPIWVRFVLVPGLTDDLDDLARIA
                     EFASGLGNVERVEVLPFHQLGRFKWEKLGLDYALASVQPPTAQATELAIDVFRKFGLT
                     AH"
     gene            1787..>3291
                     /locus_tag="VHR64_14715"
     CDS             1787..>3291
                     /locus_tag="VHR64_14715"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX3304136.1"
                     /translation="MLALFAAVGIGYALGRLSVAGFALDVGAVLFAGLALGAIAPRSA
                     PPALVSSIGLVMFLYGIGIQYGRQFFAGLGGTGLLWNALGAVAVFVALAVSVAGGAAF
                     GVSPAHTLGMFAGALTSTPTLQAAIDAAGNRAPAIGYSVAYPVGIIIPILCIFIFSKL
                     VNARLASAPAPPAITEILFAAHRESATVADVTAALPPGVHLVAIRHGRQNRLPDPLIR
                     LDAGDGLLLFGEPAALEKAREQIGPAPFPSETLTTDRSALDIARFYVSRAALAGVAIE
                     RMTFPDGVDAKIVEVRRGDASLLADQSLTLEYGDRVSVIASPKAMPALRRHFGDSIKS
                     TTEVSYVSIGLGMSFGILLGLVTVPIPGLGQFSLGLAGGPLVVALVLGRLGRTGPFAW
                     HMPLPANLTLRTFGLTLFLAAVGLSSGAPFVETLAREGFLLLFIGAAIILSAILLGLF
                     VGQWVMRLPTDDLLGVIAGITGNPAILTYANKLQPSDRVDAAYAMLFPTMT"
BASE COUNT          585 a         1092 c          999 g          615 t
ORIGIN      
        1 gagtatctcg atttcgacga cgtgctcggc aagttcgaca agatgatgga ctggctggcg
       61 ggcgtctatg tcaacgccat gaacgtcatc cactacatgc acgacaagta cgcgtacgaa
      121 cgcgtcgaga tggcgctgca cgactacgcg ccgctccgaa ccatggcgtt cggcattgcc
      181 ggcttatcgg tcgtggccga cagcctgtcg gccatcaagt acgcgaaggt gagggtcatt
      241 cgagataaga cgggcctcat caccgattac cagatcgagg gcgagttccc gatgttcggc
      301 aacaacgaca accgcgtgga ccagatcgcc agctacctcg tgagcctctt catgacgaag
      361 ctgcgcaagc atccaaccta caggaatgcg ctgcacacgc agtcgatcct caccatcact
      421 tcgaatgtcg tgtacggaaa ggcaaccggc aatacgccgg acggccggcg ccgcggcgag
      481 ccgtttgcgc ccggcgccaa tccgatgcac ggacgcgaca gccacggcat ccacgcctcc
      541 gccgcatcag tggccaagat cccgtaccgt gacgctgccg acggtatctc tctgaccacc
      601 acgctggtgc cgagcgggct gggccgcacc gcagaggatc ggattacaaa ccttaccggc
      661 atcctcgacg ccttcttcag cgcgaccggc taccacatga acatcaacgt gctgaaccgg
      721 gacacgctgg tggacgcgat ggaacacccc gagaagtacc ccagcctgac gatccgcgtc
      781 tcgggctacg ccgtgaactt cgtgcgcctg acgcgcgagc agcagatgga tgtgatcaat
      841 cgcacgttcc atggagaggc cagtgtctga gccggccgcc gttccgctcg aagccaagag
      901 cccgtacgaa ctgcgggttc acctcggaga aggcgttccc gaacagagtg tccgtgacgc
      961 gctggccacg ggcgacatgg gattcctcca ttcgtacacg acaggatctg ccgtcgacgg
     1021 gcctggactg cgggtcgtgg cgtggacaac cggctgcatg tggcgatgcc agtactgcca
     1081 caaccctgac acgtggacgc tgacaaacgg tctgcccgta tcagtgtcga atgcggcagc
     1141 cgaactgctc aagtaccgtc aaggcctcaa ggtgatgaag ggcgggttca cgctgagcgg
     1201 cggcgatccg ctgatgcagc accgcttcgc ggccaagctg ctcgcggccg tcaagcagat
     1261 gggcattcac accgcgatcg agaccaacgg tttctacggc gatcgattgt ccgatgccga
     1321 actcgacacg atcgatctcg tcatgctcgg aatcaagtcc tgggatccgg cgcgtcacct
     1381 ggcgttgacc gggaagccga tcgaaccgac gctcgaattc gcaagaaggc tcgccgcgcg
     1441 gaaacgaccg atatgggtcc gattcgtcct ggtacctggg ctcaccgacg atctcgacga
     1501 cctcgcgagg atcgcggagt tcgcatcggg actcgggaac gtcgaacggg tcgaggtgct
     1561 gccgttccac cagttgggcc gattcaagtg ggaaaagctc ggcctcgatt atgcgctcgc
     1621 cagcgtgcag ccgccgacgg ctcaagccac cgaactggcg atcgacgtct ttcgaaagtt
     1681 cggcctcaca gcacactagc cagcggctgg ctgcgcgaac gctgcaccat tctcggtcgg
     1741 aggcaccctg ctggacgccc tcaggtctct actcgagcac tctccgatgc tggcgctgtt
     1801 tgccgcggtc ggcatcggct acgcgctcgg acgcctctcg gtcgctgggt ttgccctcga
     1861 tgtcggtgcc gtgctgtttg ccggcctcgc actcggtgcc atcgcaccgc gatcggcacc
     1921 gccggcactc gtcagttcga tcggcctcgt gatgtttctc tacggcatcg gcatccagta
     1981 cggacggcag ttcttcgccg ggctgggcgg cacgggactg ctgtggaatg cgctgggcgc
     2041 ggtggcggtc ttcgtcgctc tggcggtgtc agtggccggc ggagccgcgt tcggcgtatc
     2101 tcccgcccac acgctgggca tgtttgccgg cgcactgacc agcacgccga cccttcaggc
     2161 cgccatcgac gccgccggaa accgcgcgcc cgcgatcgga tactcagtcg cctatcccgt
     2221 cggaatcatc atcccgatcc tgtgcatctt catcttctcg aagctggtga acgcgagact
     2281 cgcatctgcc cctgccccac cggcaattac cgagattctt ttcgccgccc accgagagag
     2341 cgcgacagtt gcggatgtca ccgctgctct gccgcctggt gtgcacctcg tcgccatccg
     2401 ccatggacgg cagaaccgcc tccccgatcc gctgatccgc ctcgatgccg gcgatggcct
     2461 gctgctcttt ggtgaaccgg ccgcgctcga gaaggcccgc gagcagattg gaccggcgcc
     2521 ttttccatca gaaacgctga caaccgaccg cagtgctctg gacatcgctc gcttctacgt
     2581 gtcccgggcc gcattggccg gcgtcgctat tgagcggatg acattccccg acggtgtcga
     2641 cgcgaagatt gtcgaagtcc ggagaggcga cgcgtcgctg ctcgccgacc agtcgctcac
     2701 gctcgaatac ggagatcggg tgtcggtaat cgcctcgcca aaggccatgc ccgcactgcg
     2761 gcggcacttc ggcgactcga tcaaatcgac cacggaagtg tcgtacgtgt ccatcggact
     2821 gggcatgtca ttcgggatcc ttctcggcct agtgacggtt ccgatccctg gactgggcca
     2881 gttttctctt ggcctggcgg gaggtcccct cgtagtggcg ctggttctcg gacgcctcgg
     2941 ccgcaccggt cccttcgcct ggcacatgcc gttgcccgca aatctgacgc tccggacctt
     3001 cggcctgacg ctgttcctgg ccgctgtcgg cctgagttca ggagcgccgt tcgtggagac
     3061 gctggccagg gaaggatttc tgctcctgtt cattggcgcg gccatcatcc tgtcggccat
     3121 cctgctaggg ctgttcgttg ggcaatgggt aatgagactg ccgaccgacg acctgctggg
     3181 tgtcatcgcc ggcatcaccg gtaaccctgc catcctgact tacgcgaaca agctccagcc
     3241 gagcgaccgc gtcgatgccg catacgccat gctgttcccc acgatgacga t
//
LOCUS       DATAOZ010000588         5118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3913708, whole genome shotgun sequence.
ACCESSION   DATAOZ010000588 DATAOZ010000000
VERSION     DATAOZ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3913708"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VHR64_14720"
     CDS             <1..285
                     /locus_tag="VHR64_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304137.1"
                     /translation="GEATIWLAEPATAAEHRRLASSMTERVDEEYRAIASEARAVAGL
                     PPLQRQRSLARLRRELRRVASREYFPSAEARSARDAVEALAREIANEAAA"
     gene            282..692
                     /locus_tag="VHR64_14725"
     CDS             282..692
                     /locus_tag="VHR64_14725"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX3304138.1"
                     /translation="MIWATRRHVHIDRTACAWLIQRFVDSDAEFVFVDDPDEVPAEAT
                     PFDMRAVELGHRDGRCSFESVLVRYQLDDPALERIGQLVHEADIGDERYDAPEAAGID
                     TLLRGLALSARDDQAMIDQTAPLYDALYDLWSHS"
     gene            740..1090
                     /locus_tag="VHR64_14730"
     CDS             740..1090
                     /locus_tag="VHR64_14730"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX3304139.1"
                     /translation="MVRPSEREAFWFWLKLGCISFGGPAGQIAIMHDELVERRHRISE
                     RRFLHALNLCMALPGPEAQELASYVGYSLYGVRGAITSGGLFVLPSFVLLCVMSGIYV
                     EFGDVTIVEGVQAV"
     gene            1285..1410
                     /locus_tag="VHR64_14735"
     CDS             1285..1410
                     /locus_tag="VHR64_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304140.1"
                     /translation="MCLTAADDRLEQVVIEGGKCLGGQASFYGHLVNGFKQSFDC"
     gene            complement(1599..2012)
                     /locus_tag="VHR64_14740"
     CDS             complement(1599..2012)
                     /locus_tag="VHR64_14740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3304141.1"
                     /translation="MTDELPHTQLFIPCTDTIDVLVVDDYPAVADMVCRTLEAVGCDV
                     WLADSGETALELIPEHSWDVLVTDLNMPGLSGLELLRRRDAHLPAILMSAEAITDIRF
                     ELKALNAAWLKKPFSAAQLIELVMSRAQREHDLAG"
     gene            complement(2324..3088)
                     /locus_tag="VHR64_14745"
     CDS             complement(2324..3088)
                     /locus_tag="VHR64_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3304142.1"
                     /translation="MTTTDMTQNGHQADQAHTEPFVYELERVSKIYGSGNSRVVAIED
                     IDLTIRPGEFVAVVGASGSGKTTLLQLLGALDRPTSGSITCLGRPLGDMSERELTLLR
                     RRSIGFVFQQFNLIPTLTASQNVEAKLAVASDGASEQVVELLAAVGLKGRANHLPSQL
                     SGGEQQRVAIARALSTNPSVLLADEPTGNLDTTTGQEILQLLRALSNEHAQTVILITH
                     DRELAASAPRMIELRDGRIISDERNRLEGERQEVAP"
     gene            complement(3105..4598)
                     /locus_tag="VHR64_14750"
     CDS             complement(3105..4598)
                     /locus_tag="VHR64_14750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3304143.1"
                     /translation="MFFVSYVFAELRRRRGRTILTALGLAVGVALVIAVNALSTGLDR
                     AQAKVLEPLTGVGTDMSVTRPIKITQGGGGPFGNLSRSEQRRLRNEQGGGGPLNFRDL
                     KPGSKVDQDSFQASQLSFSQSQVAKTEQLPGVAGAAGALTLNDVHIHGKAPKIQLDQG
                     SGQSGQGSQGPPAGGSFGNSSLHFDSRSVTGIDQTKSDLAAVTPSQIVKGHFFASGAA
                     GATQAILSSSYANTNKISVGDTITLDNHDFTVVGIASSPLGGTASDIYVQLAKLQKIS
                     GYKNQINTLQVRATSTSTVTSVAREIKQSLKSSEVTTAQSLAKRVSGSLTDTKNLSSK
                     LGTGLEVVGLLAAVLIASLLALSSVAKRTRELGTLKAIGWSSFQVVRQIASESVLQGL
                     LGGAIGIVLGLVGVYAINAIDWTLKATVAGSNANQAGPGTGGGPFGFGQTAVTSGSTL
                     VKITTSADAKLILVAVCLAVLGGLVAGVAGGLRAARLRPAAALRTVE"
     gene            complement(4803..>5118)
                     /locus_tag="VHR64_14755"
     CDS             complement(4803..>5118)
                     /locus_tag="VHR64_14755"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX3304144.1"
                     /translation="LLPSLRAVWVGLSGGSMVMTPRIGEDFFGWKPPAGDDSTLGIVD
                     FSIFPHLDHELLPENTMADAQKWAAGIPGPAYAIDDETAIKVADGTVEVISEGHWRLF
                     PR"
BASE COUNT          870 a         1606 c         1711 g          931 t
ORIGIN      
        1 ggcgaggcga cgatctggct ggccgagccg gccaccgccg ccgagcaccg ccgactcgcc
       61 agctcgatga ccgaacgcgt cgacgaggag taccgcgcga tcgccagcga ggcccgcgcg
      121 gtggcgggct tgccaccgct gcagcggcag cgatctctgg cacggcttcg cagggagctg
      181 cggcgagtcg cctcgcgcga atacttcccg tctgccgagg cccgctccgc gcgtgatgcg
      241 gtggaggcgc ttgcccgcga gatcgccaac gaggcggcgg cgtgatctgg gcgacccggc
      301 gacatgtcca catcgaccgc accgcctgtg cctggttgat ccagcgcttc gtcgactcgg
      361 acgccgagtt cgtgttcgtt gatgaccctg acgaggtgcc ggctgaggcg acgccatttg
      421 acatgcgtgc cgtcgaactc ggccaccgcg acggacgctg ctcgttcgag agcgtcctcg
      481 tccgatacca gctcgacgac ccagcgcttg agcggatcgg gcaactcgtc cacgaggccg
      541 acatcggcga tgagcgatac gacgcccccg aggcggccgg catcgacacg ctgctgcgcg
      601 gcctcgcgct gagcgcgcgc gacgaccaag cgatgatcga ccagaccgcg ccgctctacg
      661 acgcgctcta cgacctgtgg agccacagct gacgaccacc cagtcacctc gtcagccaga
      721 gcaggctgac ctgtcacagg tggtgcggcc gagtgagcgg gaggcgttct ggttctggct
      781 gaagctgggg tgcatcagct tcggcggccc agccggccag atcgcgatca tgcatgacga
      841 gctggtcgag cgccgccacc ggatctcgga acggcgcttc ctgcacgccc tcaacctctg
      901 catggccctg cccggccccg aagcccagga gctcgcttcc tacgttggtt actccctcta
      961 cggcgtgcgc ggcgcgatca ccagcggtgg gctgttcgtg ctgcccagct tcgtactgct
     1021 ctgtgtaatg agcggcatct atgtggagtt cggcgacgtc accatcgtcg agggcgtgca
     1081 ggccgtctga tggtatcgag ccggtcctgc gcttcgagtg ttagctggcg cgttctccgc
     1141 agcctcgctg aacaggatct cgatctgacg aatcagtgcc gggcgatcct ggtgactttg
     1201 tattccgatc gtgtggaggc cggcgatcaa tgaccgaagc gcgagcgcga gccgcgcgga
     1261 cggggcggcc cgggaccaaa ttccttgtgt ttgaccgcgg cggatgatcg gctcgagcaa
     1321 gtcgtcatcg aaggcggcaa gtgcctcggc ggtcaggctt cgttctacgg ccacctggtc
     1381 aacggcttca agcagagctt cgattgttga gtgcagcgcc tggtcggccg aaccgtgctc
     1441 gacggcgtgg cgcacgaacg agtggccaaa ctggcagtac aagcctgacg gcgtttcggc
     1501 acgagaccga cggtgagcca cctgtgcttt cgtggaaagg ttgctcccgg taggcgcccg
     1561 acggcggcgc gacggtgcaa cggtggtggc tgtcattgct aacccgccaa gtcgtgctcg
     1621 cgctgcgctc ggctcatgac aagttcaatc agctgcgcgg ccgagaacgg ctttttcaac
     1681 caagctgcgt ttagcgcttt gagttcgaaa cggatatcgg tgattgcttc ggcgctcatc
     1741 aggatcgcgg gcaggtgcgc gtcgcggcgg cggagcagtt cgaggccgga aaggccgggc
     1801 atgttgaggt cggtaacgag cacgtcccag ctgtgctcag ggatcagctc aagggccgtc
     1861 tcgccagagt cagcgagcca gacgtcgcat cccacagcct cgagtgttcg acataccatg
     1921 tcggcgacgg ccgggtagtc gtcgacgacc aggacgtcga tagtatcggt gcacggaatg
     1981 aaaagttgcg tgtgtgggag ctcgtcggtc atcattgact ccttgtcgcc gggtcagaac
     2041 gaattcggtt ggtgtgcctc ttctgcctag aggctaggag tgacggttga gtctccgatt
     2101 ccgggaagct gtgagttacc tgatacctca ttcgcggtcg gggatagctg caagacatcg
     2161 ggttatcgcg tgcggacggc tgctcgaaat gtctgcggtc gtctgctaac tcagccgccg
     2221 ttcgaggagc cgcctggtgg gcccaaggcg ccctgcctgg accgtggtgc caagacgagg
     2281 gcggcaacgg gcaggacaac gagtccgata gcgaggtgtc gtctcatggg gccacctcct
     2341 gccgctcacc ctctaggcgg ttacgttcat ccgaaatgat ccggccgtcg cgaagctcga
     2401 tcatccgtgg tgccgaggcc gccagttcgc ggtcgtgagt gatcaggata actgtctgcg
     2461 cgtgctcgtt cgagagcgct cgcaggagtt ggaggatctc ttggccggtc gttgtgtcga
     2521 ggttgccggt cggctcgtca gcaagcagca cgctcgggtt tgtcgagagc gctcgcgcga
     2581 tcgctacgcg ctgctgctcg ccgccggaga gctgtgaggg caggtgattg gcgcggcctt
     2641 tgaggccgac cgcggccagt agttcgacca cctgctcgct ggccccgtca ctcgcgaccg
     2701 cgagtttggc ctcaacgttc tggctggccg tcaaggtcgg gatcaggttg aactgctgaa
     2761 acacgaaacc gatgctgcgc cggcgcagga gggtgagctc gcgctcgctc atatccccca
     2821 gcggccggcc cagacaggtg atcgagccgc tagtcgggcg atcgagcgcg cccagtagct
     2881 gcaggagcgt cgtcttgccc gaaccgcttg ctccgacaac ggcgacgaac tcgccgggac
     2941 gaatcgtcag gtcgatgtct tcgatggcaa ctacgcggga gtttcctgag ccgtagatct
     3001 tcgagacgcg ttcgagttcg tagacgaacg gctcggtatg tgcctggtcg gcttggtggc
     3061 cgttttgggt catatcggtg gtagtcaacg tgatctcctg tgatctattc gacggttcga
     3121 agggcagctg ccgggcgaag ccgtgcggca cggaggccgc cagcgacacc ggcgacaagg
     3181 ccgccgagca cggctagaca gacagcgacg aggatcagct tcgcgtcggc ggatgtcgtg
     3241 atcttgacca gcgtcgatcc cgacgtgact gccgtctgcc cgaaaccaaa gggccctccg
     3301 ccggtaccag gtcccgcctg attggcgttt gagccggcga cggtcgcctt gagcgtccag
     3361 tcgatcgcgt ttatcgcata gacaccgact aggccgagca cgattccgat tgctccgccc
     3421 aggaggcctt gcaggactga ttcgctggcg atctggcgga cgacctggaa gctcgaccag
     3481 ccgatcgcct tcagcgtgcc cagttcgcgg gtgcgcttgg ccacagacga gagtgccagc
     3541 aagcttgcga tcagcaccgc cgcgagcaac cctacgacct cgaggccggt gccgagcttc
     3601 gacgacaggt tcttcgtatc ggtcagcgac ccagagacgc gtttggccag gctctgggcg
     3661 gtggtcacct ctgagctctt caggctctgc ttgatctcac gtgcgacaga ggtaacggtg
     3721 ctcgtcgagg ttgcgcgcac ctgaagagtg ttgatctggt tcttgtagcc ggagatcttc
     3781 tgcagcttgg cgagctggac gtagatgtca gaggcggtgc ctccgagcgg gctggaggcg
     3841 atgccgacga ccgtgaagtc gtggttgtcg agggtgatcg tgtcgccgac ggagatcttg
     3901 ttggtgttgg cataggagct tgaaaggatc gcctgcgtcg cgccggctgc gccggaagcg
     3961 aagaagtgac ccttgacgat ctggctcggc gtcacggccg caagatcgga cttggtctgg
     4021 tcgatgccgg tcaccgagcg ggagtcgaag tgcagcgagc tattgccaaa gctgccaccc
     4081 gccggcggcc cctgagagcc ctggccgctc tgtccactgc cctgatcaag ctggatcttc
     4141 ggcgccttgc catgaatgtg cacgtcgtta agcgtcagag cgccggccgc cccagcgacg
     4201 cccggcagtt gctccgtctt tgccacctgg ctctgcgaga aggacagctg cgacgcctgg
     4261 aacgagtcct ggtcgacctt cgagccgggc ttcagatccc gaaagttgag tggcccaccg
     4321 ccaccctgct cgttccgcag ccgccgctgc tccgaccggg agagattccc gaacgggcca
     4381 ccgccgccct gggtgatctt gatcggacgc gtcaccgaca tgtcggtgcc gactcccgtc
     4441 aacggctcaa gcaccttcgc ctgagcgcga tcaaggccgg tcgagagcgc gttaacggcg
     4501 atcaccagcg ccacaccgac agccaacccc aacgcggtca agatcgtgcg gccacggcgg
     4561 cggcgaagct cggcgaacac ataggacaca aaaaacacgg cgactccttc gatagtggac
     4621 ggcttcctga gtaaagccgc ccaaaatcgg aaagccgcaa gaacatgctg agaatcggct
     4681 gagcgccatg ccattccgtg ccggtcatcg acccggagcg ctgagaccgg ttgttgaggc
     4741 cggtggcggt tgggccggtt ggtgccgccg gtgccgctca ggccaccaac gcacggcacg
     4801 tctcagcgag gaaacagcct ccagtgcccc tccgatatga cctcgacggt gccgtcggcc
     4861 actttgatcg cggtctcgtc gtcgatcgca tacgccggac cagggatgcc ggcggcccac
     4921 ttttgtgcgt cggccatggt gttctccggc aggagttcgt gatccaggtg cgggaagatc
     4981 gagaagtcga cgatgcccag cgtgctgtcg tcaccggcgg gcggcttcca gccgaagaag
     5041 tcctccccga tgcggggggt catcaccatg cttccgccgc tcagccccac ccagaccgcg
     5101 cgcagcgacg gcaagagg
//
LOCUS       DATAOZ010000589         6106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5754487, whole genome shotgun sequence.
ACCESSION   DATAOZ010000589 DATAOZ010000000
VERSION     DATAOZ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5754487"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VHR64_14760"
     CDS             complement(<1..488)
                     /locus_tag="VHR64_14760"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX3304145.1"
                     /translation="MDTPTSQAQANGPSISLEECLSQLAAGTFAFGQLASFSDLSREK
                     ARTVEAAWPRIDAATRQRLIAEAIDLADENVQFRFDRLCRLALHDEDAEIRRLAVTGL
                     WEDETRSLLDEMIALARDDASIDVRAAAIALLGDALVRLSDDEPEPDLVERIGNLVLA
                     CAS"
     gene            complement(457..2142)
                     /locus_tag="VHR64_14765"
     CDS             complement(457..2142)
                     /locus_tag="VHR64_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304146.1"
                     /translation="MLSVEVAYKGSAADKELAGKMFQALNITGRFMAFDAPITMTLPD
                     LVELLGKLGTQAAEGEIQTAAKKNDAIFRIETNDEGVVSIATTRAGRAPAATSIDTSH
                     SFAKRFMTPLPKPEKPAYVARPQTVIEPIWVSDDATTAAVEEAVAAAEEALQTAAAEA
                     VVESEEVPAEAPAPAARTITVQPLRITPLDEIDDIDVAKAINERLSLDPRVATFADQW
                     MLEDRVQRFGRNDLRRIREYIQEQEQPLTDDVIAQDVLGGRPGTQEFDQLRFAVNFRL
                     SKEHRDFDFVGTANQRFWSVSSLAQLGTTRKKPNEIGTDYRHLNDELSEQPKFRTRES
                     VERVLTFYEYQLGLLPYDLEMQELLPAPLLPNQRAAVLTFECPQSYTTYLVELRYPSP
                     NRGGFIVGLDDFYNENLVPGAMISITRTENDGHYIVKYVASNSQSAKLLELEDRRQRY
                     VFRDDTYACGVLDDYLLTEERVPGIAGEKPMDEKARRRLENVIAVGFERIERKLESGT
                     GFAATFDELFAVTNIERPVTESQLRTILDTDETGAFSRDPEVADGYTYVPSAS"
     gene            complement(2185..2337)
                     /locus_tag="VHR64_14770"
     CDS             complement(2185..2337)
                     /locus_tag="VHR64_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304147.1"
                     /translation="MSTENEQQAKPVQLTEDDVAFLLSILRDPSQTQPITTQQLIDAL
                     RGRIVQ"
     gene            2530..2799
                     /gene="tatA"
                     /locus_tag="VHR64_14775"
     CDS             2530..2799
                     /gene="tatA"
                     /locus_tag="VHR64_14775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX3304148.1"
                     /translation="MLGGLGWQELVIVLIIVVIIFGAGRLPEIGGAVGKSIKEFRTQS
                     AEDEDEAGELTSTTTSTTTATGERVNTTIVEPKPTGQRDVRADEI"
     gene            complement(2900..3220)
                     /locus_tag="VHR64_14780"
     CDS             complement(2900..3220)
                     /locus_tag="VHR64_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304149.1"
                     /translation="MGIPAIVHILGEDAIYGELDQMPSPTDQFVFLRNMRQKDGKPLN
                     YLMDGAQAFLYAWHKITFIELMTDVAMPATNGTGPSTSNIGATGNLKPAGTTVLGFFR
                     DDEL"
     gene            complement(3234..5300)
                     /locus_tag="VHR64_14785"
     CDS             complement(3234..5300)
                     /locus_tag="VHR64_14785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX3304150.1"
                     /translation="MPVQYAKTEAPLIEPGKDDGRGRRLHIPGRNNLLKVGTVLQNRY
                     RVLDIRGVGGMSTVYKARDLRFTSVDRLCAIKEMFNSAEDAKLRQVRLANFHREASLL
                     ATLTHSAIPRIYDQFEQQGTIYLVLELIHGEDLETLLSQNGGPFDQEKVVDWGIALCS
                     VIAYLHNQRPEPIIFRDLKPSNVMIRAEDDSLMLVDFGIARTFAPQQKGTMIGTEGYA
                     PPEQYKGIADARGDIYALGATLHHLVTGSDPRSETPFTFNQRPPRKYNQAISQNLEEL
                     ILRCVSYSPTDRPQSAEDVKHILEQIKYSLHAPQAQVDTPSPTPTRAIQAVAAPERER
                     SAGSQLLGDGPARADDSPIKEVLPAEPSGGNSIAEKKLDWSLKTGDEVRGSAAFAGGS
                     IYLGSYDGHLYALDDADGSVRWRFKCQRGIVSRPAVFAEMVVFGSEDKNVYAVSRSSG
                     RAIWSHRTDAPIRSSPFVDDKSCIIGSDDGFLYRIDRVKGVVTWRYKTWGPIRSSPTV
                     ADDKVFFGSDDGYMYTVNRETGGLIWRYQVGSPVMSSPVVVGGQVIVGASDGAIRAFS
                     ARDGKVQWTVQTGKAVLASPAVNDDVIYIGSADGHMYAIKVATGVVVWKQRICRQITS
                     TATIDGDRLYVGGYEAIFYCIKLEDGSVEWSFGTGGPIVYHALLTDDHIVFGSFDGTI
                     YALNRE"
     gene            5557..>6106
                     /gene="lepB"
                     /locus_tag="VHR64_14790"
     CDS             5557..>6106
                     /gene="lepB"
                     /locus_tag="VHR64_14790"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX3304151.1"
                     /translation="MDELPSQSRRNLDHLGQRDDLGSVPDGYTAGPDPLRSAWDSDAP
                     ALADDQQVNPGKSAARAVREILETVLLAAVIFFGVRMLVLNFKVDGLSMMPNLQNHEL
                     LLVNRNAYEDIDLNHWLNKLPFVDREGTWVVYSFDPPHRGDIIVFNPPNGDTKPYIKR
                     VIGTAGETIEIHDGNVYVNGIKL"
BASE COUNT         1075 a         1942 c         1928 g         1161 t
ORIGIN      
        1 gacgcacagg cgagaaccag attgccgatg cgctcgacca ggtccggctc cggctcgtcg
       61 tcactgagcc gcacgagcgc gtcgccgagc aaggcaatgg ccgccgcgcg cacgtcgatc
      121 gaagcgtcgt cgcgcgcgag cgcgatcatc tcgtcgagga gggatcgcgt ctcgtcttcc
      181 cagagtccag tgacggccag ccgtcgaatc tcggcatcct cgtcgtgcaa cgcaagacgg
      241 cagagccgat cgaaccggaa ctggacgttc tcgtccgcaa gatcgatcgc ctcagcgatc
      301 aaccgttggc gcgtggctgc atcgatccta ggccacgctg cctcgaccgt gcgcgccttc
      361 tcacgcgaca ggtcggagaa cgacgcgagc tgaccgaagg caaaggtgcc tgccgctagt
      421 tgcgacaggc actcttcaag actgatcgat ggcccgttag cttgcgcttg ggacgtaggt
      481 gtatccatca gcaacctcgg ggtcccgcga gaacgcgccg gtctcgtctg tgtcgaggat
      541 cgtgcgcaac tgggactccg tcacggggcg ctcaatgttc gtgacagcga acagctcgtc
      601 gaaggtcgcg gcgaatccgg tgccggattc gagcttgcgc tcgattcgct cgaagccaac
      661 cgcaatcacg ttctcgagcc gacgccgagc cttctcgtcc atcggcttct cgccggcgat
      721 gcccggaacg cgctcctcgg tcagcaggta gtcatcgagc acgccgcacg cataggtgtc
      781 gtcccggaag acatagcgct gccgccgatc ttcgagctcg agcagcttgg cgctctggga
      841 attggacgcg acgtacttga cgatgtagtg gccgtcgttc tcggtccgcg tgatcgagat
      901 catcgcgccg ggcaccaggt tctcgttgta gaagtcgtcc aggccgacga tgaatccacc
      961 gcggttcggc gacggatagc ggagctcgac caggtaggtc gtgtatgact gcgggcactc
     1021 gaaggtcagg acagccgccc gctggttcgg aagcagcggc gccggcagga gctcctgcat
     1081 ctcgaggtca tacgggagca gcccgagctg gtactcgtag aacgtcagga cacgctcgac
     1141 gctctcgcga gtgcggaatt tcggctgctc ggagagctcg tcattcaggt gccggtaatc
     1201 ggtgccgatc tcattcggct tcttgcgggt cgtgccgagc tgggccaggc tgctgacgct
     1261 ccagaagcgc tggttggccg tgccgacaaa atcgaagtcg cgatgctcct tggagaggcg
     1321 gaagttgacc gcgaagcgaa gctgatcgaa ctcctgcgtg cccgggcgac cgcccagcac
     1381 atcctgtgcg atcacgtcgt cggtcagcgg ctgctcctgc tcctggatgt actcgcggat
     1441 gcggcgcaga tcgttccgac cgaagcgctg aacccgatcc tcaagcatcc actggtcggc
     1501 gaaggtggca acgcgcggat cgagcgacag acgctcattg atcgccttgg cgacgtcgat
     1561 gtcatcgatc tcgtcgagcg gtgtgatgcg cagcggctgg accgtgatcg tgcgcgcggc
     1621 gggcgcgggc gcctcggccg gaacctcttc gctttcgacg accgcctcgg cggcagccgt
     1681 ctgcagcgcc tcctcggcgg cggcaacggc ctcttcgacc gcggcggtgg tcgcatcgtc
     1741 cgaaacccag atcggctcga tgaccgtctg cggccgtgcc acgtaggccg gcttctcggg
     1801 cttcggcagc ggcgtcatga accgcttcgc gaacgagtgg ctggtgtcga tcgacgtcgc
     1861 cgcgggcgct cggccggcac gcgtggttgc gatcgagacc acgccctcgt cgttcgtctc
     1921 gatccggaag atcgcgtcgt tcttcttcgc ggcggtctgg atctcgccct cggccgcttg
     1981 cgtgccaagc ttgcccagca gctcgaccag atcggggagc gtcatcgtga tcggcgcatc
     2041 gaacgccatg aatcgcccgg tgatgttcag ggcctgaaac atcttgccgg cgagttcctt
     2101 atcggcggca gaccccttgt acgccacctc gacagaaagc atgggttgcc ccttctcctt
     2161 gcgtgaaaat gcggaatccg acgcctattg cacgatccgg ccgcgcaacg cgtctattaa
     2221 ctgctgggtt gtgattggct gggtttgcga tggatcgcgc agaattgaaa gcaggaatgc
     2281 aacgtcgtct tccgtcaatt gcactggctt ggcttgctgc tcgttttcgg tgctcatgcg
     2341 tccctatgcg gtgattctcg cgggctgcga gcggttgatc aacctgcgaa ctatagcggt
     2401 tccgggccgg atttcccacc gcccgcgacc acgattggca ggggctggac acggttcgta
     2461 tactgcccgc gtatcggatt tgcatctacg cgatctggct tcagatcacc tcggtaagga
     2521 cggtttctca tgctcggcgg tctcggatgg caagaacttg tcatcgtact catcatcgta
     2581 gtcatcatct tcggcgccgg ccgccttccc gaaatcggcg gagcagtcgg caagagcatc
     2641 aaggagttcc ggacgcaatc cgctgaggat gaggatgagg ccggcgagct gacctcgacc
     2701 accaccagca ccacgacggc gactggcgag cgcgtcaaca cgaccatcgt cgagccgaag
     2761 ccgacgggcc agcgggacgt ccgcgcggac gagatctagg cacgcaacac cgatcaaatg
     2821 caaaagcgcc gggcaaatcg cccggcgctt ttctgttgac gtcgatccgg gcggctcgcc
     2881 cggaattaca cgttcggagc tacagctcgt cgtcccggaa gaagccgaga accgtcgtgc
     2941 cggccggctt caggttgccg gtcgcgccga tattcgacgt cgacgggccc gttccgttgg
     3001 tggccggcat cgcgacatcg gtcatgagct cgatgaacgt gatcttgtgc cacgcgtaga
     3061 gaaatgcctg cgcgccgtcc atcaggtagt tgagcggctt gccgtccttc tgccgcatat
     3121 tgcgcaggaa gacaaactgg tcggtcgggc tcggcatctg atcgagctcg ccatagatcg
     3181 catcttcgcc caggatgtgg acgatcgccg gaattcccaa agattctccc tgcctattcc
     3241 ctgttcagtg cgtaaatcgt tccatcgaag ctgccaaaca cgatgtggtc gtcagtcaga
     3301 agtgcgtgat agacgatcgg gccaccagtt ccgaacgacc attcgacgga gccatcctcg
     3361 agcttgatgc aatagaaaat ggcctcgtag ccgccgacgt acaaccgatc gccgtcgatc
     3421 gtggctgtcg acgtgatctg ccggcaaatc cgctgcttcc agaccaccac accggtcgcc
     3481 accttgatgg cgtacatgtg accgtcggcc gatccgatgt agatgacgtc atcgttcacc
     3541 gcgggcgaag ccaggacagc cttgccggtc tggacagtcc actggacctt gccgtcccgg
     3601 gcgctgaatg cgcggatggc gccgtccgac gcgccgacga ttacctggcc accgaccacg
     3661 acgggcgacg acatgaccgg cgaaccaacc tggtaccgcc agatcagtcc gcccgtctcc
     3721 cggttgaccg tgtacatgta gccatcgtcg gatccgaaga agaccttgtc gtccgcgacg
     3781 gtcggcgacg agcggattgg tccccacgtc ttgtatcgcc acgtgacaac gcccttcacc
     3841 cggtcgatcc ggtaaaggaa tccatcgtcc gagccgatga tgcagctctt gtcgtcgaca
     3901 aagggagatg agcggatcgg ggcatcggtg cgatgcgacc agatcgcccg cccgcttgag
     3961 cgcgagacgg cgtagacgtt cttgtcctcg gaaccgaata cgaccatctc cgcgaagacg
     4021 gcggggcggg agacgattcc gcgctggcac ttgaagcgcc accgcacgga cccatcggcg
     4081 tcgtcgagcg cgtacaggtg accgtcgtat gagccgaggt agatcgaccc gccggcaaac
     4141 gcggccgagc cgcgcacttc gtcgccagtc ttcagcgacc agtcgagctt cttttcggca
     4201 atgctgttgc cgccgctcgg ctcagctggc aggacctcct tgatcggcga gtcgtccgcg
     4261 cgagccggcc catcgcctag cagctgggag ccggcggacc gctcgcgttc cggggcggcc
     4321 acggcctgga tcgcccgggt cggcgtcggc gagggcgtgt ccacctgtgc ctggggcgcg
     4381 tgcaagctgt acttgatctg ttcgaggatg tgcttgacgt cctcggccga ttgcgggcga
     4441 tccgtcgggc tgtatgagac acagcggagg atcagctctt ccaggttctg cgatatcgcc
     4501 tggttgtact tccggggcgg ccgctggttg aacgtgaacg gcgtctcgga gcgaggatcg
     4561 ctgcccgtga cgagatgatg cagggtcgcg ccgagcgcat agatgtcgcc gcgcgcgtct
     4621 gctatgccct tgtactgctc gggcggcgca tagccctcgg tcccgatcat cgttcccttc
     4681 tgctggggcg cgaaggtccg ggcgataccg aagtcgacca gcatcagaga gtcgtcctcg
     4741 gcccggatca tgacattgct cggcttgagg tcgcggaaga tgatcggctc gggccgctgg
     4801 ttgtggaggt acgcgatcac cgaacagagg gcgatgcccc agtcgacaac cttctcctgg
     4861 tcgaacggac caccgttctg gctgagcagc gtctccaggt cctcgccgtg gatcaactcg
     4921 agaacgaggt agatcgtgcc ctgctgctcg aattggtcgt agatgcgcgg gatcgccgaa
     4981 tgggtcaatg tggccagcaa cgaggcctcg cgatggaagt tcgcgagccg cacctgccgc
     5041 aacttggcgt cctcggccga gttgaacatc tccttgatcg cgcagagacg atcgacgctg
     5101 gtgaagcgaa ggtcccgcgc cttgtagact gtgctcatgc cgccgacgcc gcggatgtca
     5161 agcacgcggt aacggttctg cagcacggtg ccgaccttga gcaggttgtt ccggccggga
     5221 atgtgcagcc gccggccgcg gccatcgtcc ttcccgggct cgataagcgg tgcttcggtt
     5281 ttcgcgtact ggactggcac agcgctcctc ttgcccggtc catacccggc gtcgagatcc
     5341 ctaacctgat tcgtcgacgc cattgcatgc cggctagaca cgtcccattc tagggagcgg
     5401 ggaaggttcg taatagtcat acgcatggcc cattgggccg acttgtggct ttagtaccgt
     5461 gcggtgagcc gcgctggtgg cgctgggggt ccgagtggga tagtatccac cggcgtaccc
     5521 tgttgcggtc aatcgcgaga aacgtgacgt atcttcatgg atgaacttcc ctcccagtct
     5581 cgccggaacc ttgatcacct gggccagcga gacgatctcg gcagcgtgcc cgatggatac
     5641 actgccggcc ctgatcccct ccgttccgcc tgggatagcg acgctccggc tcttgccgac
     5701 gaccagcagg tcaatccagg caagtccgcc gcccgggctg tccgcgagat cctcgagacg
     5761 gtcctgctgg ccgcggtcat cttcttcggc gttcggatgc tcgtgctcaa cttcaaggtc
     5821 gacggcctga gcatgatgcc gaacctgcag aaccacgagc tgctgctcgt caaccgcaac
     5881 gcctacgagg acatcgacct caaccactgg ctcaacaagc tgccgttcgt cgatcgcgag
     5941 ggcacctggg tcgtctacag tttcgaccca ccccaccgcg gcgacatcat cgtcttcaat
     6001 ccccccaacg gcgacaccaa gccgtacatc aagcgcgtga tcggcacggc cggcgagacg
     6061 atcgagattc acgatggcaa cgtctacgtg aacgggatca agctcg
//
LOCUS       DATAOZ010000590        11242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8650219, whole genome shotgun sequence.
ACCESSION   DATAOZ010000590 DATAOZ010000000
VERSION     DATAOZ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8650219"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..623)
                     /locus_tag="VHR64_14795"
     CDS             complement(54..623)
                     /locus_tag="VHR64_14795"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX3304152.1"
                     /translation="MTTATSSIVTIGYGARSLDEFLDVLGQHGVSYLIDVRRWARSRY
                     KPEFSRPSLEPALAERGITYVHMGETLGGIPDDAGSYVEGKVDYERVAESESFRSGLA
                     RLRTIHEKGLPAALMCSEGKPELCHRSKLIGEALAREGIVISHIDPDGTLVSQPDVML
                     RITGPQLSMGADLAPPLRSRKKYVPSGSE"
     gene            752..979
                     /locus_tag="VHR64_14800"
     CDS             752..979
                     /locus_tag="VHR64_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304153.1"
                     /translation="MSRKPLVPGEFGAWLLNVRTNYLRWTPPQLAYYADVPLWLIEGL
                     ESGQIQDTDSSNKRKIVKAVEIAYQGPGMRS"
     gene            1099..1437
                     /locus_tag="VHR64_14805"
     CDS             1099..1437
                     /locus_tag="VHR64_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304154.1"
                     /translation="MTTHRADPHWIPDNPDGSGWSEVDWVSTIRGTRYHYRHSEPRAE
                     AERRRPGWYCVVTRLDTPQLHAPHENRYYVPAAALADFLSELILAGGDEEIWHIEPCP
                     EPPAEAHPQP"
     gene            complement(1438..3159)
                     /locus_tag="VHR64_14810"
     CDS             complement(1438..3159)
                     /locus_tag="VHR64_14810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3304155.1"
                     /translation="MDPEISHAAPESTDPDPLPLAPTFALLYVAAVALLAWRIGSRPG
                     FSYNWETYTLRDLFTFVAHPSRDVLRMNDGLMTDSGRTATVVGPAWLGFKIFGQSWFG
                     LRFPIVLISALAVPLTWVFGRRLYSDAVGITAALLVLTSQVFLLYGRTGTVVGLSIGP
                     ALIGYLLLWMCVRENDQHWLRWLLVFQIALILNSYVYSPIRFLWLIAAVLFAVECVLR
                     GGQRGRFLVSLIVTLAVLPLALSVLRPGPIETPVAAVEAYYNGRGEQIFRMSGTQSGV
                     APFLRNLTPEQQEQIAEESTDQQIRRLVRQNANDLVDLLIDRNTRPAVTDFWNPHGRL
                     YPRVLVPFFLVGMLSLLILFFFDVRARLMLALFWGYSLPLLLTTQVHIGRLVFIVPLL
                     AIICALPIGLIVRWLAARQPPQYRPAFHRWAAVAVGLLIAVAGAAPSMADWDTPFPMQ
                     RMPLVADRIVSLTNTPPTQQLVYVFGDMGGYEIETLRIAELRMELPGYLRFEDMTTGE
                     ISGTGPIPLIYGAIIPLLNQPDAIPGYCANLYLVEPDVLDTFHQASDATAQQACGQPL
                     RFDVLDV"
     gene            3284..4057
                     /locus_tag="VHR64_14815"
     CDS             3284..4057
                     /locus_tag="VHR64_14815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3304156.1"
                     /translation="MGNALAGKWALVTGSTRGLGRTTAEWLAREGAAIIVSGRDEAPV
                     AESVAAIKSIGPDAIGITADLAVHAEAHRLAKAAMAAVPQIDILVNNAGMSIRGNFWD
                     VTDEEWDYQVNVNVRSPYILAQHVAKQMIGREIRGRIVNISTIGVHACHKDAAVYNLA
                     KAGVEAMTRNMAFELGPYGISVNCVAPGNMAIRPGQAEQPWFADAARAIPYGRLGHAE
                     DIAAAVKFFCLPESGFTTGQTLLVDGAHDSYLNERIPAK"
     gene            complement(4453..5061)
                     /locus_tag="VHR64_14820"
     CDS             complement(4453..5061)
                     /locus_tag="VHR64_14820"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX3304157.1"
                     /translation="MTSTTDTFPSAIAFSRLRQGIHGSRIIIRLWADSDAEALFAAID
                     NARSHLKPWMDWVDRHQEPADTRDSITRWLIEFTRRENIALGIFDRADNRTVLGATGF
                     HNIDWTVPAVEVGYWVIPEVQGQGYVTEAVALLTDFAFAEFDANRISIQCDPHNERSK
                     RVAERLGYQLEGRLRNKARTPDGGLRDTLVYSLVPGDPRIQR"
     gene            complement(5061..6212)
                     /locus_tag="VHR64_14825"
     CDS             complement(5061..6212)
                     /locus_tag="VHR64_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX3304158.1"
                     /translation="MITHDEILDRQRRAREAADAAGYDALLVIGRSFYDRPGDLAYLT
                     NHFPPFPTTVFTGEHRGLGHALFLLPVLGAPVLITDPRRYRADLVPVEDVRASSNLMA
                     ATIGVIRERGLESAKIGLIGDDILPAPMYRELRDELPNVQIEPEPTLLSRMRMIKSPA
                     EQEAMRLAARAADAALQASIATIQRGRATERNTCAAGIYAAMSAGADFVRYMRVHSGP
                     WSALGSRWPQATEREIQRGDLVILDAIGAVHGYQFDVNRSLPAGPTDADRLQLLETVY
                     RALEASIDACRAGNRICDVVDAAKAVIDRSPFANAFGGMMGHGIGLETVEEPLLTGGN
                     ETELEAGMVLCVEPGLFVPNWGGALIEQEVIIRDAGPPEVITQTPAWLW"
     gene            complement(6255..7064)
                     /locus_tag="VHR64_14830"
     CDS             complement(6255..7064)
                     /locus_tag="VHR64_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX3304159.1"
                     /translation="MSPTLDRRRAIVSLAGIGAAGFATSRTAFAQSTPAAEATPADGA
                     AIVVTVTGSASAPATHAIGQIVMRAQYNPMPPDDPTAASDVAAPEVGAADVQAVVDAL
                     VAQGIDQAQILTNINSAGVGGGMFGPGTAVVVFQLDGEQIKTLPDAYEAATAVVTERG
                     LSFDPPGAMLLADTCQDLRGEAFRDAVVQGTDEAMLMADALGVGLGELKLARKQSVSF
                     GPAAYGYTSSDACEDLVNLGTAVRSFLPAWDATLPNEFTVYAMVELTFATA"
     gene            7252..7704
                     /locus_tag="VHR64_14835"
     CDS             7252..7704
                     /locus_tag="VHR64_14835"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX3304160.1"
                     /translation="MSTEIRTEIEIAAPADQVWDVLQDLAAYPEWNPFIREIAGPIEE
                     GARLNVRLQPPDGHGMRFRPRVVEVAPGRALRWLGHLGIPGLFDGEHRFLIEPVAPDR
                     VRFVQEERFSGLLVAVILRFAGKGTRQGFESMNAALKSRVEQHEAQAA"
     gene            7701..8258
                     /locus_tag="VHR64_14840"
     CDS             7701..8258
                     /locus_tag="VHR64_14840"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX3304161.1"
                     /translation="MTAPRSESTDETRRSPRATAIVVAARRILEQEGRDALTMRRLGA
                     EVGIRAPSLYKHFPDKSAVEVALIEIGFEEMARALESAAGHPGTLSALAAAYRTWALA
                     HPHLYRLLTSGPLPRERLRPGVEARAAAPIQAVTGDPDRARACWAFAHGMVILELDGR
                     FPPGADLDAAWAAGPSALEGDRPSG"
     gene            8318..9145
                     /locus_tag="VHR64_14845"
     CDS             8318..9145
                     /locus_tag="VHR64_14845"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX3304162.1"
                     /translation="MTERRPGQPHWIDRLAGTLAQGDKPRVSRRLLGTAGALAVAGLT
                     SAVHRKEPARAMLAAETDSILSDAATAEGLLVTMLGVARTKQSQLALDEATVRLVRAA
                     QCEEEAHFNNLVAAGAVPSTSKYTIADRVFENPTTFLTTWLDLERIMVGMYMIAARRL
                     AENGEPNLVEMAYQIGAVEAQHQALLRQLMGERLPADRAFAAWQFRQTTQGLAEIKRM
                     GLIDGDGTTYDYPGPGDRFCRGVTGLVAETTADQTPPDVTPAPRQEATPQASPVSTS"
     gene            9179..9904
                     /locus_tag="VHR64_14850"
     CDS             9179..9904
                     /locus_tag="VHR64_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304163.1"
                     /translation="MSTTPPTNEPDDEQHSIVEEEYPVDRLRTRLNESLAGRPLTVYL
                     VLFAGAATLLLLLAVVWISAQGGGDKDELICTEIATVDARAAVFSGQVQRINILVDKD
                     DPLNSLTGLQLRFNDGTCRQTPQGAALRNDLLGIIGAVDIYNKYSDASIRIHYQTQNI
                     ELELLITPTPTTVPTETPIPVSPTATPTEIPPTATATHTPLPATATATATERATPSPV
                     APTVTAEPTQAGGQRVPVGNPGT"
     gene            9987..10232
                     /locus_tag="VHR64_14855"
     CDS             9987..10232
                     /locus_tag="VHR64_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304164.1"
                     /translation="MWKRIRIADMPFWGKVLFGAGIAALLLLILITLVTGTFFATALA
                     AALLGLYLISAPFVITGAWRAPKPQPTRRPVVRRRRS"
     gene            complement(10250..10717)
                     /locus_tag="VHR64_14860"
     CDS             complement(10250..10717)
                     /locus_tag="VHR64_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX3304165.1"
                     /translation="MAISRSAHGHDETQLGLGTLLRISYQVMMAEAVEPTLAAAGYDD
                     IRSAHLPVIQALSSNPAGLRSTELATHARITKQSMGYLVDHLDNGGYVERVPDPTDQR
                     AKVVCLTQRGWELSRNIRSAVLDVEADWAGRVGAERMKQLRATLRDLVAGFER"
     gene            10856..11047
                     /locus_tag="VHR64_14865"
     CDS             10856..11047
                     /locus_tag="VHR64_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304166.1"
                     /translation="MAVTMILELQNIREPQSAITPRFLIADLPLEHLVRATPPIESAA
                     SNCLVVEDGEPTWEDAAWQ"
BASE COUNT         2057 a         3830 c         3534 g         1821 t
ORIGIN      
        1 gcggtaaagc cgtaggctcc aatggtcaca actcgttcca gtgccacgtc cgcctactcg
       61 ctcccgcttg gcacgtactt cttgcgggac cgcagcggcg gagcgagatc tgcacccatc
      121 gacagctgcg gaccggtaat ccgcagcatc acgtccggct ggctgaccag cgtcccgtcg
      181 ggatcgatgt ggctgatcac gatgccctct ctggccagcg cctcgccgat cagcttgctg
      241 cgatggcaca gctccggctt gccctcgctg cacatcaacg ccgccggcag ccccttctcg
      301 tgaatcgtgc ggagcctggc aagtccagac cgaaacgatt cagactcggc cacgcgctcg
      361 tagtcgacct tgccctcgac gtagctcccg gcgtcgtccg ggataccgcc cagcgtctcg
      421 cccatatgga cataggtgat gccgcgctcg gccagcgccg gctcgaggct cggccgcgag
      481 aattcgggct tgtaccggga tcgcgcccac cggcgaacgt cgatcaggta ggagacgccg
      541 tgctggccca acacgtcgag gaactcgtcc agcgaccgcg ccccgtaccc gattgtcacg
      601 attgatgagg ttgccgtcgt cacaatcaga aactgtaaac gccgccagcg accaacgacc
      661 aacgaccaac gaccggctat catcaccata tgggcttgag catgattgat ttgggccgcg
      721 tcaccaagac gcggccaaag ggatgtgtct gatgtcgcgg aaaccactgg tgcccggcga
      781 gttcggagcc tggctcctca atgtgcgaac caactacctg cgctggacgc cgccgcagct
      841 cgcctactac gccgatgtcc ccctttggct gatcgaggga ctcgagtccg gccagatcca
      901 ggacaccgac agcagcaaca agcgcaaaat cgtcaaagcc gtcgaaatcg cctaccaggg
      961 cccgggaatg aggtcttagg ttttgggttt caggttttgg gttttctggg ttcggttttc
     1021 ggggtacggg ccggaatcta ccggctaccg gttagtttcc cattccctca accctcaacc
     1081 ctccaccctc aactcactat gactacccac cgcgccgatc cccactggat ccccgacaat
     1141 ccggacggct ccggctggtc cgaggtcgat tgggttagca cgatccgcgg cacccgctat
     1201 cactaccggc acagcgagcc ccgcgccgag gccgagcggc gccgccccgg ctggtattgc
     1261 gtcgtcaccc ggctcgatac gccgcaactc cacgccccgc acgagaaccg gtattacgtc
     1321 ccggcggcgg cactggccga tttcctgtcc gagctgattt tggccggcgg cgatgaggag
     1381 atctggcaca tcgaaccatg cccggagcca ccggccgagg cccatccaca gccctgatca
     1441 gacgtccagc acgtcgaacc ggagtggctg cccgcacgct tgctgggcgg tagcatcgct
     1501 ggcctggtga aacgtgtcca gcacatccgg ctcgaccagg tagagattcg cacagtaccc
     1561 ggggatcgca tccggctgat tcaggagcgg gatgatcgcg ccgtagatca gcggaatcgg
     1621 acccgtcccg ctgatctccc cggtcgtcat gtcctcgaac cgcagatacc ccggcagctc
     1681 catccgcagc tcggctatcc gcaacgtctc gatctcatac ccgcccatgt cgccgaacac
     1741 gtagaccaat tgttgcgtcg gcggcgtgtt ggtcagcgag acaatccggt ccgcaaccag
     1801 cggcatccgc tgcatcggga agggcgtgtc ccagtcggcc atgctcggcg cggcgccggc
     1861 caccgcgatc aggagaccaa ccgcaaccgc ggcccagcgg tggaacgccg gccggtactg
     1921 cggtggctgt cgcgccgcca gccaccgcac gatcaatccg atcggcaagg cgcagatgat
     1981 cgccagcagc ggcacgatga agacgagccg cccgatatgg acctgcgtcg tcaacagcag
     2041 cggcaggctg tacccccaga acaaagccag catcagccgc gcccgcacat cgaagaagaa
     2101 caggatcagc agcgagagca tcccgaccag gaagaacggc accagcaccc gcggatacag
     2161 ccgaccgtgc ggattccaga agtcggtaac cgccggccgc gtattccggt cgatcagcag
     2221 atcgacgagg tcgttcgcgt tctggcggac cagccggcgg atctgctgat cggtcgattc
     2281 ttcggcgatc tgctcctgct gctcgggcgt cagatttcgc aggaacggcg cgacaccgct
     2341 ctgcgtcccg ctcatccgaa agatctgctc gccccggccg ttgtaatacg cctcgaccgc
     2401 cgccacgggc gtctcgatcg ggccgggccg gagcacgctc agcgccagcg gcaggaccgc
     2461 cagcgtcacg atcaacgaga ccaggaaccg gccccgctgc ccgccgcgca gcacgcactc
     2521 gaccgcgaag agcaccgctg cgatcagcca gaggaagcgg atcgggctgt agacataact
     2581 gttgagaatt agcgctatct ggaacaccag cagccagcgc agccagtgct ggtcgttctc
     2641 ccgcacgcac atccagagca gcaggtaccc gatcagcgct ggtccgatcg acagcccgac
     2701 caccgtaccg gttcgtccgt agagcaggaa aacctgcgag gtcagcacca gcagggcggc
     2761 ggtgattccg accgcatcgg agtagaggcg ccggccgaat acccaggtca gcggcacggc
     2821 cagcgcgctg atcaggacga tcgggaaccg cagcccgaac cagctctgcc cgaagatctt
     2881 gaaccccagc caggccggtc cgacgacggt cgcggttcgg ccgctatccg tcatcaggcc
     2941 gtcgttcatc cgcaacacat cgcgcgacgg atgtgcgacg aacgtgaaca agtcgcgcag
     3001 cgtgtaggtc tcccagttgt aggagaagcc cggccggctg ccgatccgcc atgccagcaa
     3061 cgcgaccgcc gccacgtaga gcagcgcaaa ggtcggtgcc agaggtaggg gatccgggtc
     3121 ggtcgactcc ggggcggcgt gggatatctc ggggtccatc agcgtcggtc actcgaatct
     3181 cggcaatgtg caacgcgcat cctagcaact cactgccgga tcgcacaggg cagccgtgcc
     3241 gtaggatgac gctctatcgg aataggtctc aagggagcag atcatgggca acgcgttggc
     3301 agggaagtgg gcactggtga ccgggagcac ccgcggcctg ggccggacca cggccgagtg
     3361 gctcgcgcgg gagggcgccg cgatcatcgt cagcggccgg gacgaggcgc cggtcgccga
     3421 gagcgtcgcg gcgatcaagt cgatcggccc ggatgcgatc ggcatcaccg cggatttggc
     3481 ggttcacgcg gaggcgcacc gcctcgcgaa ggcagcgatg gccgccgtcc cgcagatcga
     3541 catcctggtc aataacgccg gcatgagcat ccgtggcaac ttctgggacg tgaccgacga
     3601 ggagtgggac taccaggtca acgtcaatgt ccgctcgccc tacatcctcg cccagcacgt
     3661 ggcgaagcag atgatcggcc gggagatcag gggccgaatc gtcaatatca gcacgatcgg
     3721 cgtgcatgcc tgccacaagg acgcggccgt ctacaacctg gctaaggcgg gcgtcgaggc
     3781 gatgacccgg aacatggcct tcgagctcgg cccatacggt atcagcgtca actgcgtcgc
     3841 tcccggcaac atggcgatcc ggccgggtca ggccgagcag ccgtggttcg ccgacgctgc
     3901 ccgggcgatt ccctacggcc gtctgggcca cgccgaggac atcgccgctg ccgtcaagtt
     3961 cttctgcctg cccgaatcgg gtttcacgac cgggcaaacg ctcctcgtcg acggcgccca
     4021 tgacagctac ctcaacgagc gaatacccgc aaaatgaggc gcgcagtcgg aaggcgagca
     4081 ggcgagcagt taggttgatg gtggatggtg aatggatctc ggaaaacgga aaacgagaga
     4141 ccaaagacca aaggcgagag accagtaaaa cacggtaacg catgttacat tcgatccgtt
     4201 acgccggtct gggagtccga tcgtaccgta ccaggtatcc gactggtaca tcctttcttc
     4261 acataggtgt cctactatga attcaacgaa gtgcggcgag gaccagccac ctcagtcgca
     4321 tgggtacagg attccccgct tggaaggacc agccaccaac caagcgaccc taccccttca
     4381 ggatttgcgc cgcgcgggac cagccacctg cgcggccgca tccgtttatg ggcacctttt
     4441 ttcgccagtc tcctaccgct gaatgcgcgg atccccagga accagcgaat agaccagcgt
     4501 gtcgcgcagt ccaccgtccg gcgtgcgtgc cttgttccgc agtcggccct cgagctgata
     4561 tcccagccgc tcggccaccc gtttgctccg ctcgttgtgc gggtcgcact ggatcgagat
     4621 ccggttcgca tcgaactctg cgaaggcgaa atcggtcagc aacgcgaccg cctcggtcac
     4681 gtacccctga ccctgcacct ccgggatgac ccagtacccg acctcgaccg ccggcacggt
     4741 ccagtcgatg ttgtggaaac cagtcgcgcc gagcaccgtg cggttgtcgg cccggtcgaa
     4801 gatgccgagc gcgatattct cgcggcgggt gaattcgatg agccaacgcg tgatcgaatc
     4861 ccgcgtgtcg gccggctcct ggtgccggtc gacccagtcc atccagggct tcaggtgact
     4921 ccgcgcgttg tcgatcgcgg cgaacagcgc ttcagcatcg gaatcggccc agagccgaat
     4981 gatgatccgg ctgccatgaa tgccctggcg cagccggctg aatgcgatcg cggacgggaa
     5041 ggtatcggtc gtgctcgtca tcaccacaac caggccggtg tctgcgtaat cacctcgggt
     5101 ggtccggcat cccggatgat cacttcctgc tcaatcagcg cgccacccca gttcggcacg
     5161 aacagccctg gctccacgca gaggaccatc cctgcctcca gttcggtctc gttgcctccg
     5221 gtcagcaacg gctcttcgac cgtctccagc ccgatcccgt gacccatcat cccgccgaag
     5281 gcgttggcga atgggctccg gtcgatcacc gctttggccg catcgacgac gtcgcagatt
     5341 cggttcccgg cccggcacgc atctattgat gcctcgagcg cccggtagac cgtctcgagc
     5401 agctgcagtc ggtccgcgtc ggtggggccg gccggaagcg atcgattgac gtcgaactgg
     5461 tacccatgga ccgcacctat tgcgtcgagg ataacgaggt cgccgcgctg aatctcgcgc
     5521 tcggtcgcct gtggccagcg cgaccccagt gccgaccacg gcccggagtg gacgcgcatg
     5581 taccggacga agtcggcgcc ggcggacatc gcggcgtaga tcccggcggc gcaggtattc
     5641 cgctcggtgg cccggccgcg ttggatcgtc gcgatcgagg cctgcagcgc cgcatccgcc
     5701 gcgcgcgcag ccaggcgcat cgcctcctgc tcggccgggc tcttgatcat tcgcatccgg
     5761 ctcaacaggg tcggctcggg ctcgatctgg acattcggca gctcatcccg cagctcgcgg
     5821 tacatcggcg ccggcagaat gtcgtcaccg atcaggccga tcttcgctga ttccagaccg
     5881 cgctcccgga tcacgccgat cgttgctgcc atcaggttgg aggatgcccg gacatcctcg
     5941 accggcacca gatcggcccg gtaccggcgc gggtcggtga tcagtaccgg cgcgccaagc
     6001 acgggcagca ggaacagcgc atgtcccagc ccgcgatgct caccggtgaa gacggtggtc
     6061 ggaaacgggg ggaagtggtt ggtcaggtac gcgaggtcac ccggacgatc gtaaaaggag
     6121 cgaccgatga ccaggagcgc gtcatatcca gcggcgtccg cagcctcccg ggctcgtctc
     6181 tgccggtcga gaatctcgtc gtgcgtgatc attcctgcag tgtaccgaac gaacacgcac
     6241 gacgtggagc agatctaggc ggtggcgaag gtcagctcca ccatcgcgta gaccgtgaac
     6301 tcatttggca gcgtcgcatc ccacgccggt aggaagctgc gcaccgccgt gccgaggttg
     6361 accagatcct cacaggcgtc gctcgacgtg tagccgtatg ccgccgggcc gaacgagacc
     6421 gactgtttgc gggccagctt caactccccg agtccgacgc cgagggcatc cgccatcagc
     6481 atggcctcgt ccgtaccttg cacgaccgcg tcccggaagg cctcgccgcg caggtcctgg
     6541 caggtatcgg ccagcagcat cgcgccgggc ggatcgaagg acagtccacg ctcggtcacg
     6601 accgcggtgg ctgcttcgta cgcatccggc agggtcttga tctgctcgcc atcgagctgg
     6661 aagacgacga cagccgtgcc cggcccgaac atgccgccac cgaccccggc gctattgatg
     6721 ttggtcagga tctgcgcctg atcaatcccc tgcgcgacga gcgcatcgac gactgcctgc
     6781 acgtccgcgg caccgacctc gggtgccgcc acatcggacg cggcggtcgg atcatcgggc
     6841 ggcatcgggt tgtattgggc gcgcatgaca atctggccga tggcgtgggt tgccggcgcg
     6901 gaggccgatc cagtcacggt cacgacgatc gccgcgccgt cggccggagt cgcctcagcc
     6961 gccggggtgc tctgggcgaa ggcagtgcgg gatgtggcga aaccggccgc gccgatgccg
     7021 gccagtgaga cgatggcgcg gcgacgatca agggtagggg acatggtgga agactcctgt
     7081 gtgttgcgtt ctcggtggga ccgccaattg tgcgatccgg aatcaaaacg aagcgctgac
     7141 cgaatttgtt tccgcgaaca gggcaccagt accggggact tgacaggcta acactgttcg
     7201 cctacgttag agctaacgtt gttagcatgc tgtgcaggaa tggagaagcc agtgtctacc
     7261 gagatccgaa ccgaaatcga aatcgccgcc cctgccgatc aagtctggga tgtcctgcaa
     7321 gacctcgccg cctatcccga atggaatccc ttcatccgcg agattgcagg tccgatcgag
     7381 gaaggcgctc gcctcaacgt ccgcctgcag ccacccgacg gccacggcat gcgcttccgg
     7441 ccacgggtgg tggaggtcgc gccaggtcga gccctgcgtt ggctcggcca cctcggcatc
     7501 ccgggcctgt tcgacggcga gcaccgcttc ctgatcgagc cggtcgcgcc ggatcgcgtc
     7561 cgattcgtcc aggaggagcg cttttccgga ctgctcgtgg ccgtgatcct ccgcttcgcc
     7621 gggaagggaa cccgccaggg ctttgagtcg atgaatgccg ccctcaagag ccgggttgaa
     7681 caacacgagg cccaggccgc atgaccgccc cgcggtcgga atcgactgac gagacccggc
     7741 ggagcccgag agcaacggcg atcgtggtcg cggcgcgccg gatcctcgag caggaggggc
     7801 gcgacgcgct cacgatgcgc cgactgggcg cggaggtcgg gatccgtgcc ccctcgctct
     7861 acaagcactt cccggacaag tcggccgtgg aggtcgccct gatcgagatc gggttcgagg
     7921 agatggcccg ggcgctcgaa tccgccgccg ggcaccccgg cacgctatcg gccctcgcgg
     7981 cggcatatcg cacctgggcg ctcgctcatc cgcacctgta tcgcctgctt acctccggcc
     8041 cgttgccgcg cgaacggctc cggccggggg tggaggcacg ggccgccgct ccgattcagg
     8101 cggtgaccgg cgatccagac cgggcgcgag cctgctgggc attcgcccat ggcatggtca
     8161 tcctcgagct cgacggtcgc ttcccgcccg gcgccgacct cgatgcggcc tgggctgccg
     8221 ggccttctgc cctggaagga gaccgaccca gcggttagcg atatccggcg caacaggtat
     8281 gatgtgtacg gacacttcct tcaggttgag ggacgcgatg acggagcgca gaccagggca
     8341 accgcattgg atcgaccggc tggccggcac cctcgcccag ggcgacaagc cccgggtcag
     8401 tcgccgcctg ctcggcacgg ccggcgcgct cgcggtcgcc gggctgacgt cggcggtgca
     8461 ccggaaggaa ccggcacggg caatgctcgc cgcggaaacc gattcgatcc tgagcgatgc
     8521 cgcgacggcc gagggactcc tcgtcaccat gctcggcgtc gcgcgcacca aacagtcgca
     8581 gctcgccctc gacgaggcca ccgtccgcct ggttcgagcc gcccagtgcg aggaagaggc
     8641 gcacttcaac aacctcgtcg cagccggagc ggtcccatcg acgagcaaat acaccatcgc
     8701 ggaccgggtt ttcgagaatc ccactacgtt cctaacgacc tggctcgatc tggaacggat
     8761 catggtcggc atgtacatga tcgccgcgcg gcggctggcc gagaacggtg agccgaacct
     8821 cgtcgagatg gcctaccaga tcggcgcggt cgaggcccag caccaggccc tactgcgaca
     8881 gctcatgggc gagcgcctgc cggcggaccg ggcattcgcc gcctggcagt ttcggcagac
     8941 cacgcaaggg cttgcggaga tcaagcgcat gggtctcatc gacggcgacg gaaccacata
     9001 tgattacccc gggcccgggg atcgattttg tcgcggcgtg accgggctcg tcgccgagac
     9061 aaccgccgac cagactccgc ctgacgtgac ccccgcaccg cgtcaggagg caacgccgca
     9121 agcgtcgccc gtctcaacgt cgtagccact cgcgggattt cgccagggac agtcaggttt
     9181 gagcacgacg ccaccaacga acgagccaga cgacgagcaa cactcgatcg tcgaggagga
     9241 gtacccggtt gatcggctcc ggacgcggct gaacgagtcg ctggccggac gtccgctgac
     9301 cgtctatctc gtcctcttcg ccggtgcggc gacgctcctg ctcctgctcg cggtggtatg
     9361 gatctcggcc cagggtggcg gcgacaagga cgagttgatc tgtaccgaga tcgccactgt
     9421 cgatgcccgg gctgccgtct tcagcgggca ggtgcagcgc atcaacatcc tcgtcgacaa
     9481 ggacgacccg ctcaacagcc tgaccggcct ccagctccgc ttcaacgatg gcacctgtcg
     9541 gcagactccg cagggcgccg ccttgcgcaa tgatctgctc gggatcatcg gagcggtcga
     9601 tatctataac aagtacagcg acgccagcat ccgcatccac taccagaccc agaacatcga
     9661 gctggagctg ctgattacgc cgacgccgac gacggtcccg accgagacac ccatccctgt
     9721 gtcgccaacc gcaacgccaa ccgagatccc gccgaccgct accgccacgc atacaccgct
     9781 gccggcaacc gcaaccgcga cggccaccga gcgggcgacg ccatcacccg tggcgccgac
     9841 ggtaactgcc gagccgaccc aggctggggg tcagcgggtt ccggtcggca atccgggcac
     9901 ctgacatttg ccccatatgt cccccgcaca cgaatagcgc aggcatcgcg gggtcattac
     9961 tgtggtgcgg gagaggagac aggccgatgt ggaaacggat ccgaatcgct gacatgccgt
    10021 tttggggcaa ggtgctcttt ggagccggaa tcgccgcgct cctgctcctg atcctgatca
    10081 cactcgtgac gggcacattc ttcgcgacgg cgctggccgc cgcgctcctt gggctgtacc
    10141 tgatctcggc gccgttcgtg atcaccgggg cctggcgggc gccgaaaccg caaccgaccc
    10201 gtcgtcccgt tgtccgtcgt cgtcgctcgt agcgccgcct tcgccccgat caccgctcga
    10261 agccggcaac cagatctcgt agagtcgccc gtagctgctt cattcgctcg gctccgacgc
    10321 gcccggccca atcagcctcg acgtcgagga ccgcggaccg gatgttccgg ctcagctccc
    10381 agccgcgctg cgtcaggcaa acgaccttcg cccgctggtc ggtcgggtcc ggcacccgct
    10441 cgacgtagcc gccattatcc aggtggtcga cgagatagcc catcgactgc ttggtgatcc
    10501 gcgcatgcgt cgccagctcc gtggaccgca ggccggccgg gttgctactc agggcctgga
    10561 tcaccgggag atgcgcgctc cggatatcgt cgtagcctgc cgcggcgaga gtcggctcga
    10621 ccgcctcggc catcatcacc tggtacgaaa tgcggagcag cgtgcccagc ccgagttggg
    10681 tctcgtcgtg tccatgggca gacctggaaa tcgccatctc tgtacaccct cttgacaaaa
    10741 tggtcagata gtcttaccgt atcgtcagat aggcttacta catgctacca cacggcgtga
    10801 ccagtgacag tgcggatcga ccggctgtcg gtgcatgacg aacggagatc acgagatggc
    10861 cgttaccatg atccttgaat tgcagaacat ccgcgagccg caatcggcga tcactccgcg
    10921 gttcttgatc gccgacttgc cgctcgagca tctggtccgg gccaccccgc ccatcgaatc
    10981 ggccgcatcg aattgcctgg ttgtcgagga cggcgagccc acctgggaag acgccgcgtg
    11041 gcagtagcga aaaggagaca atcatgaccc agtccgcaga cctcgtagcc attaatccgg
    11101 ccgaagagac gatccgcctc gggccgctcg ggatccgttt cctcgtcacg ggggcggact
    11161 cccgcggaag cgctgccgtg ttcgagtgct cggttcccgc cggcgagcaa ctccgggcac
    11221 ccgcgcatag ccacgacgcc ta
//
LOCUS       DATAOZ010000591         3433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_493615, whole genome shotgun sequence.
ACCESSION   DATAOZ010000591 DATAOZ010000000
VERSION     DATAOZ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493615"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..2822
                     /gene="ppdK"
                     /locus_tag="VHR64_14870"
     CDS             195..2822
                     /gene="ppdK"
                     /locus_tag="VHR64_14870"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX3304167.1"
                     /translation="MGRWIRLFTEGDAGQRALLGGKGANLAEMTQLGLPIPPGFTVTT
                     EACLEYLRNNGRIPEGLWEEVEYGIRYIEGRMGRRFGDPDKPLLLSVRSGAAASMPGM
                     MDTILNIGLNDTTTDSLGAVMGERFALDAYRRLIQMFGKVVLGAPSEKFDDALSGARL
                     ALGVKSDHELTPVALRRLIIHFREILHAQEAGLPDDPVEQVREAILAVFRSFNTERAI
                     SYRQMHGLSENMGTAANVQAMVFGNMGSDSATGVAFTRDPNTGAPGLFGEFLQNAQGE
                     DIVAGVRTPRPVSEMFSDAAFRAAGAQLQAIADQLEAHFTDMQDIEFTVEQGRLWILQ
                     TRTGKRTAQAAVRIAVDLAEAGSIDRREAIHRVSPTQIELLLHPHIDSTRHIEVVATG
                     LPASPGAATGTVVLNTAEARRRGEAGEAILLVRPETAADDFPGMRYAQGILTARGGMT
                     SHAAVVARGMGTPAVTGCSALEIDERAGVIRVGSTVIRAGDDLTIDGSTGHVILGKAA
                     MIQAHLDERAERLLSWADETRRMKVRANADTPADAQRARGLGAEGIGLCRTEHMFFTN
                     GRLPVIRTLILSEDRHERERALAKLERFQTDDFASIFRAMDGLPVTIRTLDPPLHEFL
                     PTRPEEIAKIADRLNMPIEEIEDRIDGLHEANPMLGHRGCRLGMTVPEITEMQARAIF
                     RAALRCAAEGVVVLPEIMIPLVSDIEELRRQRRLIDGVAGQLFEQAGRSVEYHVGTMI
                     ELPRAALRGADLAQEADFFSYGTNDLTQTTYGLSRDDAGTFLPQYLVDGVYPKDPFQV
                     LDRDGVGELIQIASERGRSANPTLELGVCGEHGGDPESIQFCNEIGLDYVSCSPFRVP
                     IARLAAAHAALGSEAAVIV"
     gene            complement(2893..>3433)
                     /locus_tag="VHR64_14875"
     CDS             complement(2893..>3433)
                     /locus_tag="VHR64_14875"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX3304168.1"
                     /translation="TVSIQSAFDAGEEVASLIMIAAGGLLTVFAMWWLYFDQPTDGSL
                     RRFWRGEAGLRESPFVFGYGHLFIFASAAAVGGGLEIAVDFATHHTEISGPQAAAMIA
                     GPVALYLACIWVLRFTLHQIPILKTIMYASAICLVLCTIVLVQPVLPIGLVSAGLVTA
                     LVLVRWRIESRASVPQPAD"
BASE COUNT          628 a         1085 c         1166 g          554 t
ORIGIN      
        1 gttatccacc ggtgtttgca ccgcgataga aatgcaaaga ttcgctgtaa gacgttaaca
       61 ccgaggcggt tgccatgaaa gtttgtgaga aagtattcac aagtccccat gtaagttgct
      121 atcatgccgg cctgaggcac tcggagcggc gtggctcgga tcgtccgacc ggattggtgc
      181 aaggagacaa gaccgtgggc cgatggattc gattgttcac cgaaggcgat gccggccagc
      241 gcgctctgct cggcggcaag ggtgcgaacc tggcggagat gactcagctc gggttgccaa
      301 ttccgcccgg ctttaccgtc acaaccgaag cctgcctcga gtacctgcgc aacaacggcc
      361 gcattcccga ggggttatgg gaagaggtcg agtacggcat acgctacatc gagggccgca
      421 tgggtcgacg gttcggcgat ccggacaagc cgctcctgct ctcggtccgc tccggcgcgg
      481 cggcctcgat gccgggcatg atggacacga tcctcaatat cggcctcaac gacaccacga
      541 ccgacagtct gggggcggtg atgggcgagc ggttcgcgct cgatgcctat cgccgactga
      601 ttcagatgtt cggcaaggtg gtcctgggag cgccgtccga gaagttcgac gacgcgcttt
      661 ctggcgctcg tctcgccctt ggagtcaaga gtgaccacga gctgacccca gtggcactgc
      721 ggcggttgat catccatttc cgtgagatcc tgcacgccca ggaggccggc ctgccagatg
      781 atccagtcga gcaggtccgg gaggcgatcc ttgctgtctt ccgctccttc aacaccgagc
      841 gggcgatctc ctaccggcag atgcacggtc tcagcgagaa tatgggcacc gcggccaacg
      901 ttcaggcgat ggtcttcggc aacatgggtt cggatagtgc caccggcgtc gcgttcacac
      961 gcgatcccaa cactggggct cccggtctct tcggcgagtt tctccagaac gcacagggcg
     1021 aggacatcgt ggccggcgtt cgcacgccac ggccggtcag cgaaatgttc agtgacgctg
     1081 cattccgtgc tgccggcgcg caattgcagg cgattgccga tcagctcgag gcgcacttca
     1141 ccgacatgca ggacattgag ttcacggtcg agcagggtcg gctctggatc ctccagaccc
     1201 gaaccggcaa acggaccgcg caggcggcgg tgcggattgc ggtcgatttg gccgaggccg
     1261 gctcgatcga ccgccgcgag gcgatccacc gggtcagccc gacccagatc gagctcctgc
     1321 tgcaccccca catcgattcg acgcgtcata tcgaggttgt cgcgaccgga ttgccggcaa
     1381 gtcccggcgc agccaccggc acggtggtcc tgaacaccgc cgaggccagg cggcgcggag
     1441 aggccggcga agcgatcctg ctggtccgac ccgagaccgc ggctgacgat ttccccggca
     1501 tgcgctatgc ccagggaatc ctgaccgcgc gcggcggcat gacctcgcac gcggcggtgg
     1561 tcgcgcgtgg gatgggcacg ccggcggtga ccggttgcag cgcgctcgag atcgatgaac
     1621 gagccggtgt gatccgggtc ggcagcacgg tcatccgggc cggcgacgat ctgacaatcg
     1681 acggcagcac ggggcacgtc atcctcggca aggcggcgat gatccaggcg catctcgacg
     1741 agcgggccga gcgattgctc tcgtgggcgg atgagacacg gcgcatgaag gtccgcgcca
     1801 atgccgatac gccggccgac gcgcagcgag cccgagggct cggcgccgag gggatcgggc
     1861 tctgccgcac ggagcacatg ttcttcacca acggacgctt gcccgtgatc cggacgctga
     1921 tcctgagcga agaccggcac gagcgcgagc gggcgctggc caagctcgag cggttccaga
     1981 ccgacgactt cgccagcatc ttccgggcga tggacggcct gccagtcacg atccggacgc
     2041 tcgacccgcc gctccacgag ttcctgccga cgcggccgga ggagatcgcc aagatcgcgg
     2101 accggctgaa catgccgatc gaggagatcg aagatcggat cgacggactg cacgaggcca
     2161 atccaatgct cgggcatcgg ggatgccggc tcggcatgac ggtgccggag attaccgaga
     2221 tgcaggcgcg ggccattttc cgggcagctc tgcgctgtgc ggcagaaggc gtcgtagtcc
     2281 tgccggaaat catgatcccg ctcgtgtcgg acatcgagga gctgcgtcgg cagcgtcgcc
     2341 tgatcgacgg cgtggccggc cagctgttcg agcaggcggg gcgatcggtc gagtatcacg
     2401 tgggcacgat gatcgagcta ccgcgggcgg cactgcgggg cgcggatctt gcgcaagagg
     2461 ccgacttctt cagctacggc acgaacgacc tgacccagac aacgtatggc ttgagccgag
     2521 atgatgccgg cacgttcctg ccgcagtacc tggtcgacgg cgtctacccg aaggacccgt
     2581 tccaggtcct cgatcgcgac ggcgtcggcg aattgatcca gatcgcatcg gagcgcggcc
     2641 ggtcggccaa cccgacgttg gagctgggcg tctgcggcga gcacggcggc gatccggagt
     2701 cgatccagtt ctgcaacgag atcggtctcg actacgtgag ctgctcgcca ttccgggtgc
     2761 caatcgctcg cctggcggcg gcgcacgcgg cgctggggtc cgaggcggca gtcatcgtct
     2821 aatctcgccc gtccttcaac cacgccccgg gtcctgatca atggcgatca agacccgggg
     2881 cgtttccatt cgtcagtcag ccggctgcgg gaccgaggcg cgcgattcga tgcgccagcg
     2941 gacgagaacg agggcggtga cgaggccggc cgagacgagc ccgatcggca ggaccggctg
     3001 cacgagcacg atcgtgcaga ggacgaggca gatcgcggat gcatacatga tcgtcttcag
     3061 gatcggtatc tggtgcaggg taaaccgcag cacccagatg caggcgaggt agagcgcaac
     3121 cggtccggcg atcattgcgg ctgcctgtgg cccgctgatc tccgtatggt gggtcgcgaa
     3181 atcgaccgcg atctccagtc caccgccgac tgccgctgcc gaggcgaaga tgaagaggtg
     3241 gccgtagccg aagacgaacg ggctctcgcg caggccggcc tcgcctcgcc agaaccggcg
     3301 cagggagccg tcggttggct ggtcgaaata gagccaccac atcgcgaaga cggtcaacag
     3361 accaccggcc gcgatcatga tcaggctggc cacctcctcc ccggcatcga atgcggactg
     3421 gatcgagacg gtt
//
LOCUS       DATAOZ010000592         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4705429, whole genome shotgun sequence.
ACCESSION   DATAOZ010000592 DATAOZ010000000
VERSION     DATAOZ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4705429"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VHR64_14880"
     CDS             <1..395
                     /locus_tag="VHR64_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="protein CapI"
                     /protein_id="HEX3304169.1"
                     /translation="DVYKFCRHRQDFTYIDDIVKGITRVLDRPARPNLEWRGDTPNPA
                     SSTAPYRLYNIGNHQPVELLYLVELLEKALRRKARMNLLPMQPGDVEDTVADVAALER
                     EVGFAPSTPIEEGVARFVAWYRDYHGVR"
     gene            745..>1690
                     /locus_tag="VHR64_14885"
     CDS             745..>1690
                     /locus_tag="VHR64_14885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX3304170.1"
                     /translation="MVTMLARPTAETVAGARLQERLARREAVVGLIGLGYAGLPLAVA
                     FAEAGFPVIGVDLRRDRVDSLTAGQSYVGDVSAERLNPLITSGNLRASAEYASLADAD
                     IITICVPTPLSQTKTPDTSAITGAMTALGPWLRPGQLIMLESTSYPGTTEEIVRPVLE
                     ARGFTIGQDIFLAFAPERVNPGDTHFGTRNTTKLIGGMTPACRDLATSLYATIVDKVV
                     LVSSPVVAEMAKLLENTFRQINIAFANEMALMCERLGVDVWEVIDAAATKPFGFMPFY
                     PGPGLGGPCIPVVPHYLAWKLKTVGYDARFIALAEEING"
BASE COUNT          319 a          514 c          540 g          317 t
ORIGIN      
        1 tcgacgtcta caaattctgt cggcaccggc aggatttcac ctacatcgat gatatcgtca
       61 aggggatcac ccgcgttctg gatcgtcccg cgcggccaaa ccttgagtgg cgaggcgaca
      121 cgcctaatcc cgccagcagc acggcgccat accggctgta caacatcgga aaccatcagc
      181 cggtggagct gctctacttg gtcgagctgc tggagaaggc cctccggcgc aaggcccgta
      241 tgaatctgct gccgatgcag ccgggcgatg tcgaagacac tgtcgccgac gtcgcggcac
      301 tggagcgcga ggttggcttc gcgccgtcga ccccgatcga agaaggggtc gcgcgcttcg
      361 tcgcctggta tcgcgattat cacggtgtga gatgatcttc tcgtgccctc gccatttctt
      421 aggtgtcaaa ggtgttgaag agtttaaagg gtttaaagag tttgaacttc tgaggagccg
      481 ggcgggagca ccatcttcac cgatgtcgca aaaacgaaat tatgaccttt gacgatttgg
      541 cggatcgggg atagaatcta cttcgctcac gaaggggagg gtgcattccg ccttggcagc
      601 ggacgcggcc gagcaatcac caggattccc gctgatcttg tccaatgttt catcggtgcg
      661 gcacggctgg agcgtcgtac gcgggttgcc gtattgacca atgggacgga gtgattcggg
      721 gatcatgcgg cggggaaaag cagaatggtg acgatgttgg cgcgaccaac tgcagagact
      781 gtggcgggag cacggctgca ggagagactc gcgcgacgcg aagccgtggt gggtctgatc
      841 ggactcggct acgcgggcct gccgctggca gtcgcgttcg ccgaagcggg attcccggtg
      901 atcggcgtcg atctccgccg cgatcgcgtc gattccctga ccgccggaca gtcctacgtc
      961 ggagacgtat ccgccgagcg cctgaacccc ttgatcactt ccggcaacct gcgagccagc
     1021 gccgagtatg cgtccctggc ggatgccgac atcatcacca tctgcgtgcc cactccgctg
     1081 tctcagacga agacgccgga cacgagcgcc atcaccggag ccatgaccgc gctgggacca
     1141 tggttgcggc ccgggcagct gatcatgctg gagagcacct cctacccagg gaccaccgag
     1201 gagatcgtgc gcccggtgct cgaggcgcga ggcttcacca ttggtcagga catcttcctc
     1261 gccttcgccc cggaacgggt caaccccggc gatacgcact tcggcactcg caacacgacg
     1321 aagctgatcg gggggatgac gcccgcctgt cgcgatctgg ccacttcgct ctacgcgact
     1381 atcgtcgaca aagtggtgtt ggtctcctcg cccgtcgtgg ccgagatggc gaaactgctg
     1441 gagaacacgt tccgccagat caacatcgca ttcgccaacg agatggcgct gatgtgtgag
     1501 cgcctcgggg tcgacgtctg ggaagtgatc gacgcggcgg cgaccaagcc gttcggcttc
     1561 atgcccttct accccggtcc cggactgggc ggtccctgca tcccggtggt gccgcactat
     1621 ctggcatgga agctgaagac ggtcgggtac gacgcccgct tcatcgcgct ggccgaagag
     1681 atcaacgggg
//
LOCUS       DATAOZ010000593        13707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1018491, whole genome shotgun sequence.
ACCESSION   DATAOZ010000593 DATAOZ010000000
VERSION     DATAOZ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13707
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018491"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VHR64_14890"
     CDS             complement(<1..549)
                     /locus_tag="VHR64_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304171.1"
                     /translation="MDLRLSTPNGPPALLVVDADDEARENVASALIRRFGADYQVMTA
                     DSAASGLRSLDQLAAEGVDLGLVAADYRLPGTDGIEFLAQAHIRHPAASRALLIDMDT
                     RGTRIPFGELDALQRATALGRIDFWLVNGWQSPEELLYPRVQEALSEWTRINRPQHEV
                     IRVVGELWSPRCHDLRESLSRNT"
     gene            complement(581..1681)
                     /locus_tag="VHR64_14895"
     CDS             complement(581..1681)
                     /locus_tag="VHR64_14895"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3304172.1"
                     /translation="MATNVLDVLVIGAGQAGLALGYQLKQTGQTFLLVDRYDRIGESW
                     RRRFDSLTLFTPRSYSALPGLPVSGDPNGYPTKDEIAAYLETYARHFDLPVALGTGIA
                     RLEQTGDLFRATTNAGTVLTARSVVLATGAFQQPAIPALGSRFAPDVVQLTPETYRNP
                     RQTPSGTVLVVGDGATGRQIARELSPTHRVFLSTGRSRKVSPHRVLGQSIFWWMDKLG
                     ILTKSPDSRIGRKLKEKDPFPGKDLALDKLQRGGVIVAGRLTDVDGRQVSFADGQSAG
                     IDVVIWATGYRDESDWVAIPGVKDARGGFVHDQGLSAVPGLAFIGRSWQRSRGSALLT
                     GVGADAAFIVDQLNRVAEPGADALLLPAAVGI"
     gene            complement(1800..3530)
                     /locus_tag="VHR64_14900"
     CDS             complement(1800..3530)
                     /locus_tag="VHR64_14900"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX3304173.1"
                     /translation="MDSHNMHEMHMTANDESFSTDITGLAPTTATDIVDLADGATFDL
                     VAAPVRKQIGDAELRMLAYNGSVPGPTLRVQQGSTITINFTNQTDLESTVHWHGLRLD
                     NRFDGTHETQAPVVVGGAFAYQITFPDPGVYWYHPHIREDYGQEMGLYGNILVVPAQA
                     DYWPPVHRELLLTLDDILIEDGAMASFSRTETTFSAMGRYGNVLLINGEIEPEFTAQT
                     GEVVRLYLTNTANARVFNVAVPGARIKLVGGDSGRVEQETFVESVIISPSERAVIDVL
                     FEQAGPVTIEHETPEKRSTLATVNVSAERVSEQLAGPFDVLRRNPEWTAERERFAPFF
                     DAEPDKTLSIVAEMQMDDEGATSYACPMHPEVTSDSPGRCPKCGMTLLPVAAINEASG
                     QDMHAQHGHMDHGSHMDHSAHMDPGQHGHMDHGEHAGHGQMHHGHHTDAADGIEWEDD
                     MVDVNRITTPANTRWKFIDTATGAANHAIDWRFRVGDLVKIRLINEMDSDHPMHHPFH
                     IHGAGRFVVLARDGADEQNLVWKDTVLVRTGQTVDILLEVTNPGTWMAHCHIAEHHES
                     GMMFSFTVDS"
     gene            3841..4266
                     /locus_tag="VHR64_14905"
     CDS             3841..4266
                     /locus_tag="VHR64_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3304174.1"
                     /translation="MIKALLVDDQPQILEGLEMLLALEPDLQIVGTAQTAEAAITLAH
                     ELRPDIIVMDVRLPGIDGIAATRTLGSELPDGKVVILTLYCDSETRRQAREAGAQAFV
                     AKHDMTQSLLATIRRIAAQTKQSLDEVAPQCAAHGERET"
     gene            4266..5003
                     /locus_tag="VHR64_14910"
     CDS             4266..5003
                     /locus_tag="VHR64_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304175.1"
                     /translation="MALATWWRGDERPEIAPLPGLSIDVGRDIGQIAGLTALESSEVE
                     ARIGAGHRIYIASLDGAPAAYGWVATLSASIGELSLAFSLPASDRYLWDFVTLPEWRG
                     RGIYPRLLQGIVEAEGLQAERFWIIHAPENAASGSGITKAGFEPVSELSFLPESGAGV
                     GTVVLASADRARVGADLLGVQFFEAIQSGRIVSPCWRCVIEQHAIGADGATCWPGDGH
                     GGAPSCSCVPGGAHSDAGSGCALGLAS"
     gene            5105..7192
                     /locus_tag="VHR64_14915"
     CDS             5105..7192
                     /locus_tag="VHR64_14915"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX3304176.1"
                     /translation="MHTSNPLSIPTHRALLETLHARLPHGVAILDCDLRLVYSNPRWS
                     DFMAQAGAGDPLPGASLSDLIPTAEDVQGTARLSLQSGEPCNLPGLSFPADTGESFWD
                     LTLAPLSDGDMIVGLKCVVYEATERVLAERAAAAKARLTAFRAEVSQAMAGSDDIDTV
                     LQCCAEAMVRHAPAAFARIWVLDEADDAYRLRASAGLYTRLDGTYSRVPAEWIRQKTF
                     GGHSAEVAIDILKTHHVREPEWAAANGLVAWANHPLVVTGRIIGFMSMFARQNLDPDT
                     LDELAAVADAIAQFLERKHAQIALREQLEQRVAKRTRELSLLLDISRNVISTLELKPL
                     LELILDQLRTVVPYTGTAVLTIEGDDLIIAGQRGPLSEEAARLIRYPVEKMAPVWARL
                     SRGGAIIIPDVRGDSMEADVFRAVVGADLDDRLTFIGSCLWAPLVVKDQLIGCMSITN
                     QETGAYTADHAVMAGAIARQAAVAIENARLFEQARGIAALEERQRLARELHDSVSQAL
                     FGIGLGAETAKTMLERDPAKAAGPIDYVLQLARGGMAEMRALIFELRPESLEQEGLVA
                     ALEKQATATGVRHGIEVELALAAEPDIPIEMKEALYRIAQEAMHNTAKHAEASKVTLC
                     LAIEPDALTMAVIDDGSGFDTSASFPGHLGLVSMQERITRFGGRVEIKSAPGAGTTVQ
                     ISVPLPSKSHVHA"
     gene            7207..7851
                     /locus_tag="VHR64_14920"
     CDS             7207..7851
                     /locus_tag="VHR64_14920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX3304177.1"
                     /translation="MPIRVLIADDHKVVRQGLKMFLATDPELEVIGEAPNGAEAVKLA
                     RALQPDVILMDLMMPVMDGISAIEAIRSEGIDTEILAVTSVLEDDAVVRAIRAGATGY
                     LLKDTEAAELCRAIHAAADGQVQLAPQAAARLMREVAAPKNPETLTERETEVLRLLAK
                     GLANKEIAQELSIGNRTVKSHISSILAKLDVQSRTQAALHAARIGLVDIAEVGT"
     gene            complement(7910..9490)
                     /locus_tag="VHR64_14925"
     CDS             complement(7910..9490)
                     /locus_tag="VHR64_14925"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3304178.1"
                     /translation="MQIETKYAKSGDASVAYQVIGQGPIDIVLVPCWLTNIAHNWREP
                     RFAAFLERLGSFARLITFDKRGTGLSDPAPSSEPFTLEQRMDDLTAVLDAAGSKRAVL
                     FGVAIGGRMAALYAATYPERVQGLILMETSARGSWAPDYPWGPTSEQLQWRNDQIFET
                     WGGPVWIESYAPSIQHDSRFRAWWAECLRSSAGPAMAASIFRLSAESDIRSVLPSIQA
                     PTLVMHRRGDVVAGLEEGRYLAGHIHGARFVELDGEDHLAYVGDQEPIFREISRFLAA
                     ELNTAPVRRSLGTVVSVNATGIAEFVKRAGAERWAATRASVRNDIETALRQYRGLASM
                     QQGHGLVAAFDGPTRAINFACEVLETIRRHGLHAQIGLHAGMIEYAEDRIGGVTAELA
                     TRIAMAAVRDEILTSSTLVGLVTGSCLCFAPVEDREIVGLPAGLRLFAVTDGPASTEQ
                     TIMPRTDEAVAELSPREREVAFNVAQGMSNRAVGEALSISISTVERHVANILMKLGFR
                     SRTQLSLWAAEQRLAVGD"
     gene            complement(9524..11161)
                     /locus_tag="VHR64_14930"
     CDS             complement(9524..11161)
                     /locus_tag="VHR64_14930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3304179.1"
                     /translation="MSGVIGAPGIGLTGHDVLHGFSPAHRHGKHALVRETRYARNGTA
                     SIAYQTFGSGQRDIVLGLGWLSNLDAAWEEPRYARFLSGLANFAHVVAYDARGTGLSD
                     AIPLSENLSLDERANDLKAVIDSAKLSAPTLVGFGASGYAFAQFAAKYPERVGALILI
                     NSASNGLARLTSDATGTVQVDLQTVAPSAAQEPAFTAWWEAFLRRCASPGSALSLARM
                     NAGIDIRPSLPAINAPTLVLHRVGDRAVPIAHGRELAALIPRAILRELPGEDHLPFIG
                     DSDSLIDEIEQFMTGARTRREPDVDLATILVIDVADSAEVAVAMGDRRWANVWSQVEA
                     IISDEILRDGGNQLMSLPSGVMASFPAPVPALQCAIRLNEAIRQLRLTARAGLHAGEL
                     PAGRSGRTGVVGQLALRVARLARPGEVLATDSVVGLAAGADLAFEPAGDHPMPGYPGG
                     WRLHRVGRAGAPSVPGTAPLGDGRPLAMLSPREREIAALIALGLSNRQISDELAISVG
                     TVERHVANMLGKLGYRSRTQIAAWAVEHALLSPPPAS"
     gene            complement(11231..11437)
                     /locus_tag="VHR64_14935"
     CDS             complement(11231..11437)
                     /locus_tag="VHR64_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304180.1"
                     /translation="MDDFMTLANFEIGQRHRRAERDRMIRDWRAHRAEMRRTADARTD
                     NASDSNDGIGGFAGLMQRLTRATS"
     gene            complement(11853..13157)
                     /locus_tag="VHR64_14940"
     CDS             complement(11853..13157)
                     /locus_tag="VHR64_14940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEX3304181.1"
                     /translation="MITEATMTGFETLALHGGQEPDGTTNSRAVPIYQTTSYVFNDTD
                     HAARLFGLKEFGNIYTRIMNPTTDVLEKRIAALEGGVGALAFASGQAAETMAILNLAG
                     VGDEIVSTTSLYGGTYNLFHHTLSKLGITVRFVEPTAGAVAGAITARTKAVFSETIGN
                     PDLNTLDIAAVADVAHAAGVPLILDNTLPTPYLVRPIDYGADIVVHSATKFIGGHGTS
                     IGGLVVDGGTFDWAASGRFPEFTTPDPSYHGLVYSDVFTAENFGANIAYIIKLRVQLL
                     RDVGPSMSPFNAFMFLQGLETLALRMERHSQNAMAVARFLTEHPRVTWVNYPGMPTHP
                     TYQTASKYFRGGYGAILGFGIQGGSEAGRRFIDNLSLFSHLANVGDAKSLAIHPATTT
                     HSQLTEAEQATTGVTDDYVRLSVGIESIGDIITDLDRALAAI"
     gene            complement(13267..>13707)
                     /locus_tag="VHR64_14945"
     CDS             complement(13267..>13707)
                     /locus_tag="VHR64_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304182.1"
                     /translation="GREPAARPSPYPPFGGTYDIEGYLHHQGELLVRRFDANSYLYLT
                     RAMDLYDVGRGQGDAYWLRRIAAPLLLVGIRSDWLYPPEEVRALFAQVEAAGRDATYL
                     ELDSPYGHDAFLKEWAALGGMLQEFLDRVRPGEASERGMIAQAQ"
BASE COUNT         2427 a         4497 c         4371 g         2412 t
ORIGIN      
        1 cgtgttccgc gacaggctct ctcgcaagtc gtggcagcgt ggcgaccaga gctcgccgac
       61 gacgcggatg acctcatgtt gcggacggtt gatgcgcgtc cattcgctga gcgcttcctg
      121 gacccgcgga taaagcagct cctccggtga ttgccagcca ttgaccagcc agaagtcgat
      181 gcggcccagg gcagtcgcgc gttgaagggc atcgagttcg ccgaaaggaa tccgcgtgcc
      241 gcgcgtgtcc atgtcgatca acagcgcgcg gcttgcggcc ggatgccgga tgtgtgcctg
      301 ggcgaggaac tcgatgccgt cggtaccggg caagcgataa tcggccgcga cgaggccgag
      361 gtcgacgccc tcggcagcaa gttgatcgag cgacctcagc ccggatgcag ccgagtcggc
      421 cgtcatcacc tggtaatcgg ccccgaaccg ccggatcaga gccgacgcca cgttctccct
      481 tgcctcatca tcggcatcga cgacgagcag ggcgggcggt ccgttcggcg tactgagacg
      541 gagatccatt gtccactctc tccctgcctg gatcggcgtc ctagattcca accgcggccg
      601 gcagcaacaa agcgtcagcg cccggctccg caacgcgatt caactggtcg acgatgaagg
      661 ccgcgtcggc gccgacacca gtcaacagcg ccgacccgcg gctccgttgc cagctccggc
      721 cgatgaaggc cagccccggg accgccgaca ggccctgatc atggacgaac ccgcctcggg
      781 cgtctttcac ccccgggatc gcaacccaat cgctctcgtc gcggtacccg gttgcccaga
      841 tcacgacgtc gattccggcc gactgcccgt ccgcaaacga gacctgtcgt ccgtcgacgt
      901 cggtcaaccg accggcgacg atgactccgc cgcgctgcaa cttgtcgagc gccaggtcct
      961 tgcccggaaa cgggtccttc tccttcagct tccgaccgat gcgcgaatcg ggcgacttgg
     1021 tcaggatacc gagcttgtcc atccaccaga agatgctctg gccgagtact cggtgtggac
     1081 tgaccttccg cgaccgtccg gtcgaaagga agacgcgatg cgtcgggctc agctcgcggg
     1141 cgatctggcg gccggtggcg ccatctccca ccaccagcac ggttccggac ggcgtctgcc
     1201 gcgggttgcg gtacgtctcc ggcgtgagct ggacgacgtc cggagcaaat cgactcccga
     1261 gtgccggaat ggccggctgc tggaacgcgc cggtcgcgag gacgaccgag cgagcggtca
     1321 ggacggtacc ggcattggtc gtcgcacgga acaggtcgcc tgtctgctcg agccgggcga
     1381 tcccggttcc cagggcgacc ggcaagtcga agtgcctggc gtaggtttcg aggtaggcgg
     1441 cgatttcatc cttggttggg tacccattgg gatcgccgga caccggcagg ccgggcaatg
     1501 cgctatacga gcggggcgtg aacagggtca gcgaatcgaa tcgccgtcgc cagctctcgc
     1561 cgatgcggtc gtacctgtcg accagcagga acgtctggcc ggtttgcttg agctgatacc
     1621 cgagggccag acctgcctgc ccggcgccga tcaccagtac gtcgagaaca ttggtcgcca
     1681 tttgagatct cctcgtttgc gatgcggggc gtttgcctct ctcctctagg aacgagagca
     1741 tccctgcgct acgtttggaa cagggcgggg cacggcggcc ccgcgctacc tttgcaaggt
     1801 caggagtcca cggtgaacga gaacatcatg ccgctctcgt ggtgctcggc aatgtggcag
     1861 tgcgccatcc aggtgcccgg attcgtcacc tcgagcagga tgtcgaccgt ctgaccagtc
     1921 cgcaccagca ccgtgtcctt ccagaccagg ttctgctcgt cggcgccatc ccttgccagc
     1981 acgacgaagc ggccggcgcc atggatgtgg aacgggtggt gcatcggatg gtcggaatcc
     2041 atctcgttga tgagccggat cttgaccagg tcaccgacgc ggaacctcca gtcgatcgcg
     2101 tggttggccg cgccggtcgc cgtgtcaatg aacttccagc gggtgttggc cggcgtggtt
     2161 atccggttga cgtcgaccat gtcgtcttcc cactcgatac catctgctgc gtcggtgtga
     2221 tggccatggt gcatctggcc gtgcccagca tgttcgccgt gatccatgtg tccgtgctgg
     2281 ccggggtcca tgtgggcgct gtgatccata tgcgagccat ggtccatgtg gccgtgttgt
     2341 gcgtgcatgt cttggcctga tgcctcattg atcgccgcga ccgggagaag cgtcatgccg
     2401 cacttcgggc agcgaccggg cgaatcgctc gtcacttccg gatgcatcgg gcaggcgtaa
     2461 gaggtcgcgc cctcatcgtc catctgcatc tcggcaacga tcgagagcgt cttgtccggt
     2521 tcggcgtcga aaaacggcgc gaaccgctcg cgctcggccg tccactccgg gttccgtcgc
     2581 agcacatcga acggcccggc aagctgctcc gatacccgct cggcgctgac attgaccgta
     2641 gcgagggtcg agcgcttctc cggcgtctca tgctcgatcg tcactggtcc ggcctgctca
     2701 aacagcacgt cgatgacggc ccgctccgac ggcgagatga tgacggactc gacaaacgtc
     2761 tcctgctcga cccgaccgct gtcgccaccg acgagcttga tccgggcgcc gggcaccgcg
     2821 acgttgaaga cgcgggcatt ggcggtgttg gtcaggtaaa ggcggacaac ttcgccagtc
     2881 tgagcggtaa attccggctc gatctcgccg ttgatcagca ggacgttgcc gtaccggccc
     2941 atcgccgaga aggtggtctc cgttcggctg aacgacgcca tcgcgccgtc ctcgatcagg
     3001 atgtcgtcga gcgtcagcag cagttcgcgg tgcacgggcg gccagtagtc ggcctgggcc
     3061 ggcacgacga ggatgttgcc gtaaagaccc atctcctgac catagtcctc gcggatgtgc
     3121 gggtgatacc agtagacgcc cgggtccggg aacgtgatct ggtaggcgaa ggccccgccg
     3181 accacgaccg gcgcctgggt ctcgtgcgtg ccgtcgaacc ggttgtcgag ccgcaacccg
     3241 tgccagtgaa ccgtgctctc gaggtcggtc tggttcgtga aattgatcgt gatcgtggaa
     3301 ccctgttgaa cgcggagcgt cggtccgggt accgatccgt tgtaggccag catcctgagc
     3361 tcggcatctc cgatctgctt ccggaccggc gcggcgacga gatcgaaggt cgcgccatcg
     3421 gccagatcga cgatatcggt ggcagtcgtc ggcgcgaggc cggtgatgtc ggtgctgaac
     3481 gactcgtcgt tggccgtcat gtgcatctcg tgcatgttgt gtgagtccat ctggcccgtc
     3541 tctcctctgt ggtctctcgg cggccggctg cctcgtcgtc ccggccctga ctccactatc
     3601 gggcgcagcg caaccccaaa catcgccact ctggacgcac gaacgtctgc cttttgggca
     3661 gcgatcatct ccgcccaatg acggagatcc cgccgcccag agtggcgatg ctcggcgccg
     3721 tggccgcgcc ctacgctgaa ggcattgatt cgactgatcg acggcgacag ctgcggcgcg
     3781 ccgccagaca gcacaggaat ggagggttcg gacaatgcgg ttcattgatc cgaacggaag
     3841 atgatcaagg cactgttggt cgacgaccag ccgcagattc tggaggggtt ggagatgctg
     3901 ctcgcactcg agcccgacct ccagattgtc ggcacggcac aaacggccga ggcggcgatc
     3961 accctggcgc acgagctgcg gccggacatc attgtcatgg atgtgcggct gccggggatc
     4021 gacggcatcg ccgcgacccg cacgctgggc agtgagctgc cggacggcaa ggtcgtcatc
     4081 ctcacgctct attgcgattc cgaaacgcgg aggcaagccc gcgaggcggg agcccaggcg
     4141 ttcgtcgcca agcacgacat gacgcagtcg ctcctcgcga cgatccgccg gatcgcagcc
     4201 cagacgaagc aatcgctgga cgaagtagcg ccgcaatgcg cggcacatgg tgaaagggag
     4261 acgtaatggc attggcaact tggtggcgcg gcgacgagcg gccggagatc gcacccctcc
     4321 ccggtttgag catcgacgtc ggtcgcgaca tcgggcagat cgcaggcctg accgcactcg
     4381 aatcgagcga ggtcgaagcc cgaatcggag ccggacaccg gatctacatc gcttctctcg
     4441 atggcgcgcc ggccgcgtac ggctgggtcg cgacgctctc agcgtcgatc ggcgagttat
     4501 cgctcgcgtt ttcgttgccg gcgagcgacc gctacctctg ggatttcgtg actctgcccg
     4561 agtggcgcgg ccgaggcatc tatccccgcc tgttgcaggg gatcgtcgag gcggaaggat
     4621 tgcaagcaga gcggttctgg atcatccacg cgccggagaa tgccgcgtcc ggctcgggca
     4681 tcaccaaagc cgggttcgaa ccggtcagcg agctgtcgtt cctgcccgaa tccggtgcag
     4741 gcgtcgggac cgtagtgctc gccagtgcgg atcgtgcccg ggtcggcgct gacctgctcg
     4801 gcgtgcagtt cttcgaggcg atccagagcg gccggatcgt ctcgccctgc tggcgttgcg
     4861 tgatcgagca gcacgcaatc ggcgcggatg gagcgacctg ctggcccggc gacgggcacg
     4921 gcggggcccc ctcctgctca tgcgttcccg gtggagcgca cagcgacgca ggcagtggct
     4981 gcgcccttgg acttgcgagt tgatcgcctg ataaaattcg gcatgtgaaa ttatgtaatg
     5041 cctttgatca gccgccgtcg gtcgatcgac cgacggtggc tgcgaccacg tgaacagatt
     5101 cctgatgcac accagcaatc ccttatcaat tcccactcac cgagccctgc tcgagaccct
     5161 tcacgcgcga cttccgcacg gcgtggcgat actggactgc gacttgcggt tggtctactc
     5221 caacccgcgt tggtcggact ttatggctca ggcaggagcc ggtgacccgc tccccggtgc
     5281 atccctctcc gacttgatcc ccaccgcgga agatgtgcag ggcacggccc ggctcagcct
     5341 gcaaagcggt gagccgtgca atctgccggg attgtcattc ccagcggata ccggcgagtc
     5401 gttttgggac ctcacgcttg cccccctctc cgatggggac atgatcgtcg gtctcaaatg
     5461 cgtcgtgtac gaggcgaccg agcgcgtgct ggctgagcgc gcggccgcgg ccaaggcgcg
     5521 attgaccgcg ttccgggccg aggtcagcca ggcaatggcc ggctccgacg acatcgacac
     5581 agtcctgcaa tgctgcgccg aggcaatggt ccggcacgcg ccggccgcat ttgcccggat
     5641 ttgggtcctc gacgaagcag acgatgccta ccggctgcgg gccagcgccg ggctctacac
     5701 ccggttggac gggacgtatt cgcgcgttcc ggccgagtgg atccggcaaa aaacgttcgg
     5761 tggccactcg gcggaagtcg caattgacat cctgaagaca caccacgtgc gggagccgga
     5821 gtgggctgcc gccaacggcc tggtggcatg ggcgaaccat cccctcgtcg tcacgggccg
     5881 gatcatcgga ttcatgtcca tgttcgcgcg ccagaatctc gatcccgaca ccctcgacga
     5941 gctggcggcc gtggccgatg ccatcgccca gttcctcgag cgaaaacatg cgcagatcgc
     6001 cctgcgcgag cagcttgagc agcgagtcgc caaacggacg cgcgagcttt cgctcctgct
     6061 cgacatctca cgcaatgtga tctcgacgct ggagctgaaa ccgctcctgg agctgattct
     6121 cgatcagctc cggacggtcg ttccctacac gggcacagcg gtcctgacga tcgagggcga
     6181 tgacctgatc atcgccggcc aacgtggccc gctctccgaa gaggccgcgc gcctgatccg
     6241 ctatccggtc gagaagatgg cgccggtctg ggcccggttg agccgcggcg gggcgatcat
     6301 cattccggat gtgcgaggcg actcgatgga ggccgacgtc ttccgcgcgg tcgtgggtgc
     6361 tgatctggac gatcgactga ccttcatcgg ctcatgcctc tgggcgccgc ttgtagtcaa
     6421 ggaccagcta atcggctgca tgagcatcac gaatcaggaa accggcgcat acaccgcgga
     6481 ccacgcggtc atggccgggg cgattgccag gcaagcggcg gttgcgatcg agaacgcgcg
     6541 actcttcgaa caggcgcggg gtatagccgc tctcgaggag cggcaacgtc tcgcccgcga
     6601 gctgcacgac tcggtctcgc aggcgctgtt cgggatcggc ctgggcgccg agacggccaa
     6661 aaccatgctc gagcgggatc cggccaaggc cgccggaccg atcgactacg tacttcagct
     6721 cgctcgtggc ggcatggccg agatgcgcgc gctgatcttc gagctgcggc ctgaatcgct
     6781 tgagcaagag ggactggtag cggcgctcga gaagcaggcg acggccactg gtgtccgtca
     6841 tggcatcgag gtcgagctgg ctctcgcggc cgagccggat atcccgatcg agatgaagga
     6901 agcgctctac cggatcgccc aggaagcgat gcacaacacg gccaagcatg ccgaggcgtc
     6961 gaaggtcacg ctctgcctgg caatcgagcc cgacgctctg acgatggccg taatcgacga
     7021 tgggtccggg tttgacacca gcgcatcctt cccgggtcac ctcggtctcg tctccatgca
     7081 ggagcggatc acccgctttg gcggacgggt cgagatcaag agcgcgcccg gcgcaggcac
     7141 tacggtgcag atttccgtgc cgcttccatc gaagtcgcat gttcatgcat aagtatggag
     7201 aagatgatgc cgattcgcgt cctgatcgct gatgaccaca aggttgtccg tcaggggctc
     7261 aagatgttcc tcgccaccga ccctgagctt gaggtcatcg gcgaggcgcc caacggggca
     7321 gaggccgtca agctggcccg ggcgctgcag cccgacgtga tcttgatgga cctgatgatg
     7381 ccggtcatgg acggcatctc cgcgatcgag gcgatccgct cggaagggat tgacaccgag
     7441 atcctggccg tgaccagtgt gctcgaggat gacgcggtcg tccgggctat ccgcgcgggc
     7501 gccaccggct acctgctgaa ggacaccgaa gcggccgagc tttgccgggc gatccacgcc
     7561 gccgccgatg gccaggtcca gcttgccccg caggctgccg cgcgattgat gcgggaagtg
     7621 gccgcgccga agaaccccga aacgctcacg gagcgcgaga cggaggtgct tcgtctgctc
     7681 gcgaagggtc tcgccaataa ggagatcgcg caggagctgt cgatcggcaa ccggacagtc
     7741 aagagccaca tcagcagcat tctggccaag ctcgacgtcc agtcccgtac ccaggccgct
     7801 ctccacgccg cccggatcgg cctcgtggat attgctgaag tcggaacctg agtggacggt
     7861 attggtagat ggtcgttgga aattgaatcc atctaccatc taccaactac taatcaccca
     7921 ctgccaggcg ctgctccgct gcccaaagcg acagttgcgt gcggctgcgg aagcccagct
     7981 tcatcaggat gttcgcgacg tgccgctcga cggtcgagat cgagatcgac aacgcctcgc
     8041 cgactgcccg gttcgacatc ccctgcgcga cgttgaacgc gacctcgcgc tctcgcgggc
     8101 tcagctccgc caccgcctcg tcggttcgcg gcatgatcgt ctgctcggtc gacgcgggac
     8161 cgtcggtcac cgcgaatagc cgcaaccctg ccggtagtcc aacaatctcg cggtcttcaa
     8221 ccggcgcgaa gcagaggcac gagccggtta ccagcccgac gagcgtgctc gacgtcagta
     8281 tttcgtcccg gaccgccgcc atcgcgatcc gcgttgccag ctccgccgtg accccgccaa
     8341 tacgatcttc cgcgtactcg atcatcccgg cgtgaagccc gatctgggca tgaagcccgt
     8401 ggcggcggat cgtctcgagc acctcacacg caaagttgat cgcgcgggtc ggtccatcga
     8461 acgcggcgac caatccatgt ccctgctgca ttgaagccaa accccgatat tgccgcagcg
     8521 cagtttcgat gtcgttgcgt accgatgccc tggtcgcggc ccatctctcc gcgcccgctc
     8581 gcttcacaaa ctcggcaatc ccggtcgcat tcaccgacac gaccgtcccc agcgatcgcc
     8641 gcacgggcgc ggtgttgagc tcagcggcga ggaaccggct gatctcgcgg aagatcggct
     8701 cctgatcgcc gacgtaggcg agatgatcct cgccatccag ctcgacgaag cgtgccccgt
     8761 gaatatgtcc ggccaggtat cggccctctt caaggcccgc gaccacatct ccgcggcggt
     8821 gcatgacgag cgtcggcgcc tggatcgacg gcagcaccga gcggatatcg ctctcggcgg
     8881 aaagacggaa gatcgaggcg gccatcgccg ggcccgcgct gctgcgcagg cactcggccc
     8941 accacgcgcg gaaccgcgag tcgtgctgga tgctcggcgc ataggactca atccagaccg
     9001 gaccacccca agtctcgaag atctggtcgt tacgccactg caactgttcg gacgtcggtc
     9061 cccacgggta atccggcgcc catgagccac gggccgaggt ctccatcagg atcagcccct
     9121 gtacccgctc cgggtaggtc gccgcataga gcgccgccat ccggccgccg atcgccacgc
     9181 cgaagagcac ggcccgcttc gaaccggcgg catcgaggac ggccgtcagg tcgtccatcc
     9241 gctgctcgag cgtgaacggt tccgaggagg gggcaggatc agacaagccc gtgccgcgtt
     9301 tgtcgaacgt aatgaggcga gcgaacgatc ccagccgctc gaggaaggcc gcaaatctgg
     9361 gctcccgcca gttatgagcg atattcgtca gccaacaggg cacgagcacg atgtctatcg
     9421 ggccctggcc gatcacctgg taggcaacac tcgcgtcgcc tgatttcgcg tacttcgtct
     9481 caatctgcac ggtgcactct cctgtgaact gtcacccctg ccgtcacgac gctggcggcg
     9541 gggagagaag cgcgtgttcg actgcccacg ccgcaatctg cgtacgcgag cggtatccga
     9601 gcttgcccag catgttggcc acgtgccgct cgaccgtgcc gaccgagatc gcgagctcgt
     9661 cgctgatctg acggttcgac agtccgagcg caatcagggc cgcaatctcg cgttcccgcg
     9721 ggctgagcat cgccaaaggc ctaccgtcac ctaacggtgc agtgcccgga acggatggtg
     9781 cgccggcgcg gccaacgcga tgtaatcgcc atcctcccgg atatcccggc atcgggtggt
     9841 ccccggccgg ctcgaacgcc agatcggctc cagcggccaa accgacaacc gagtcggtgg
     9901 ccagcacttc tcccggccgg gccaggcgag caacccgcaa tgccagttgc ccgaccaccc
     9961 cggtgcggcc cgatcgtcct gccggcagct cgccggcatg cagacccgcc cgagccgtga
    10021 ggcgaagctg gcggatggcc tcattcagcc ggatggcgca ctggagcgcg gggacgggcg
    10081 cggggaatga agccataacc ccgctgggca ggctcattaa ctgattgcca ccatcacgca
    10141 agatttcatc ggaaataatc gcctcgacct gggaccagac atttgcccac cgccgatccc
    10201 ccatcgcaac cgcgacctcg gcgctatcgg ccacatcgat gaccaggatg gtcgccaggt
    10261 cgacgtccgg ttcgcgccgg gtccgcgcgc cggtcatgaa ctgctcgatc tcgtcgatca
    10321 agctgtcgga gtcgccgatg aacggcaaat ggtcctctcc cggcagctcc cgcaggattg
    10381 ctctcgggat cagcgccgcg agctcgcgac catgcgcaat cggtacggca cgatctccaa
    10441 cgcggtgaag caccagcgta ggcgcgttga ttgccggcag gctgggccga atgtcgatcc
    10501 ccgcgttcat ccgagcgagg gacagcgccg aaccggggct cgcgcagcgg cggagaaatg
    10561 cctcccacca ggctgtgaat gccggctcct gtgcggcact cggcgcgacc gtctgcaagt
    10621 cgacctgcac cgttcctgtc gcgtccgatg tcaaccgggc caacccgttg cttgcgctgt
    10681 tgatcagaat cagcgccccg acacgttccg ggtacttcgc cgcaaattgc gcaaaggcat
    10741 agccgctcgc gccgaaacca accaaggtcg gggccgagag cttcgcggag tcgataactg
    10801 ctttgaggtc attggcccgc tcgtcgagac tcaggttctc ggacaacggg atcgcgtcgg
    10861 aaagtccggt acctctggcg tcgtacgcaa caacatgagc gaagttcgct aatccgctaa
    10921 ggaagcgtgc atagcgcggc tcttcccacg ccgcatcgag gttcgagagc cagccgagcc
    10981 caagcacgat gtcgcgctgg ccgctgccga aggtctggta cgcgatgctc gccgttccgt
    11041 tgcgcgcgta cctggtctcc cgcaccagcg cgtgcttccc gtggcgatgc gctgggctaa
    11101 atccatggag cacgtcgtgg ccggtcaaac caatgcccgg cgcgccgatt actccggaca
    11161 tgccaaaccc cgctccggac ggaccagcag gtgagccttt gtccaggacg gggtgtgaca
    11221 tgtcgttttc tcaggaagtc gcgcgggtca accgttgcat caagccggca aacccaccga
    11281 tcccgtcatt cgaatcggac gcattgtcag ttcgggcatc ggcggtccgg cgcatctcgg
    11341 cccggtgcgc gcgccagtcg cggatcatcc gatcccgctc ggctcgcctg tgccgctgac
    11401 cgatttcgaa gttggccagg gtcatgaaat catccatgtc gatttccatt tctgctgtct
    11461 gccgatagat ttccgatacc ggggatgtcg cccgattcgc cgacgacgcg cgcccgaacg
    11521 gcgcgctcgc gcgccgcgcg aaccgcgctg cgataccggg ggagatccat cccctcgagt
    11581 ccagcggagc caacaagctc cgtcctcgct ccgcccgaaa gtccatatcc aatcaacgca
    11641 ataaccatct gttgcacatc cttccaatgc tcacttagcg ccggaacctc atgcccggct
    11701 tgctcatcct gccagtcgcc aaaatcgact tcatccaagg aaccatgtat atgaccatgt
    11761 aaatcccgga ccccgaaaat cacatccggc aaaagacgga ggggacctcg gcatttggtc
    11821 ccctccacaa agcacatcga tttagcccgg cgctagatcg cggccaatgc tcgatccaga
    11881 tcggtgatga tgtcaccgat ggactcgatg ccgaccgaca agcgcacgta gtcgtcggtg
    11941 acaccggtcg tcgcctgttc ggcttcggtg agttgcgagt gggtcgtcgt cgctggatga
    12001 atcgcaagcg acttggcgtc gccgacgttg gccaggtgcg agaagagcga gaggttgtcg
    12061 atgaagcgtc gacccgcctc gctaccacct tggatgccga agcccaggat tgccccgtag
    12121 ccgccgcgga aatacttgct cgcggtctga tacgtcggat gagtcggcat gcccgggtaa
    12181 ttcacccagg tcaccctcgg gtgctcggtc aggaaccggg cgaccgccat cgcgttctgg
    12241 ctgtgccgct ccatgcgcag ggccagcgtc tccagcccct gcaggaacat gaatgcattg
    12301 aacgggctca tcgacgggcc aacatcgcgc aggagctgaa cacggagctt gatgatgtag
    12361 gcgatgttcg cgccgaagtt ctcggccgtg aagacatcgc tgtaaaccag cccgtggtag
    12421 ctcggatccg gcgtggtgaa ctccgggaat cgcccggacg ccgcccagtc gaacgtcccg
    12481 ccgtcgacga cgaggccgcc gatcgacgtc ccgtggccgc cgatgaactt ggtcgcggag
    12541 tggacgacga tgtccgcccc gtaatcgatt ggccgcacga ggtacggcgt cggcagcgtg
    12601 ttgtcgagga tcagcggcac cccggccgcg tgcgccacat cggctacggc ggcgatatcg
    12661 agggtgttca gatcaggatt gccgatcgtc tccgaaaaga cggctttcgt cctggcggtg
    12721 attgccccgg ccacggcccc ggcagtcggc tcgacgaacc ggacagtgat acccaacttg
    12781 gacagcgtgt ggtggaaaag gttgtaggtt ccgccataga ggctggtagt cgaaacgatc
    12841 tcatcgccca cgccggcgag gttgaggatg gccatggttt cggccgcctg accggaggca
    12901 aaggcaagcg ctccgactcc accctccagc gccgcgatcc gcttctcgag cacatcggtc
    12961 gtcgggttca tgatccgggt gtagatgttg ccgaactcct tgagtccgaa cagccgggct
    13021 gcgtgatcgg tgtcgttgaa cacgtacgag gtcgtctgat agatcggcac cgcgcgggag
    13081 ttggtcgtac cgtctggttc ctgcccgcca tggagggcaa gggtttcaaa gccggtcatc
    13141 gttgcttcag taatcacagc attctcctta gtccaggtag tcacattcat tcgccgcgtg
    13201 acatttgcag cgcggccaca cgtcgcaatt caacccatcg cgcgggcagt gatcgctgct
    13261 cgcgcgtcac tgtgcctggg cgatcatccc cctttcgctc gcctcgcccg ggcgaactcg
    13321 atcgagaaat tcctggagca tgccgccgag tgccgcccat tccttgagga aggcgtcgtg
    13381 cccgtacggc gagtcgagtt cgagatacgt ggcatcccgg ccggccgcct ccacctgggc
    13441 gaaaagtgcc cggacctcct ccggcgggta aagccagtcg gatcggatgc cgacgagcag
    13501 aaggggagcg gcgatccgcc gcagccaata cgcatcgccc tgcccgcggc cgacgtcgta
    13561 gagatccatg gcccgggtca ggtagaggta gctgttggca tcgaagcggc ggacgagcag
    13621 ctcgccctga tgatggaggt agccctcgat gtcgtaggtg ccaccgaacg gtggataggg
    13681 cgatggccgg gctgcgggtt cgcggcc
//
LOCUS       DATAOZ010000594         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5229844, whole genome shotgun sequence.
ACCESSION   DATAOZ010000594 DATAOZ010000000
VERSION     DATAOZ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5229844"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VHR64_14950"
     CDS             <1..553
                     /locus_tag="VHR64_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HEX3304183.1"
                     /translation="IIPGETACLRCVFPDAPDAGSAPTCDTAGVFGPSVHVVSSLEAS
                     EAIKLALGRTDAINRSLVSLDIWSMELQRIPVGGPRPDCPTCQQRQFDFLDRKSLNLE
                     TTLCGHDAVQVIIQPPVKLDLAALGKRLEPAGDVLVNRFLVRFTDGVTGHELTIFPDG
                     RAIVKGTTEPREARAIYDRYVGS"
     gene            560..1711
                     /locus_tag="VHR64_14955"
     CDS             560..1711
                     /locus_tag="VHR64_14955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3304184.1"
                     /translation="MIYLDNAATSWPKPESVRQAIVEALDQYGANPGRSSHQMAIQTA
                     EMIYRTRAGLARLVNGDSPERIVFCTNATDALNLAIFGLVRPGDKVVTSSMEHNSVRR
                     PLRRMQDLGAEVITVQTERSGVVDPAAIRAVADGAKLIAITHGSNVNGAVQPVAEIGR
                     IAREVGALLLIDAAQTIGAMPIDVRAMQIDLLAFPGHKSLLGPSGTGGLYIGERVNVA
                     ELEPLHAGGTGLRSEDDIQPLELPYRYESGTVNTLGIAGLAAGLKHVMTLGIEQIQAH
                     HTESRARLAAGIAESGATMFEPPMGAADVVSFDIPGWQPADVGSVLDQAFGIACRTGL
                     HCAPGACATIGAGPNGTIRFSPGLTTTGEEIDAAIEAVAAIASEAMVRS"
     gene            1708..2127
                     /locus_tag="VHR64_14960"
     CDS             1708..2127
                     /locus_tag="VHR64_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304185.1"
                     /translation="MNDPNGNPRRSRLEDEVLEILAKTDRKPPLTAKARAWGRTARAQ
                     ILKWRAHIGWLDTAWGWFGIALALFIIGGWVAKDSGLLQQVIQIAGIVAIAVAVLRLV
                     RPGSGQGRKMWRGRPMDLKKPGVELGDNKLDDWRKRR"
BASE COUNT          399 a          723 c          783 g          373 t
ORIGIN      
        1 gatcatcccg ggcgagacgg cctgcctgcg ctgcgtcttc cccgatgcgc cggatgccgg
       61 gtcggcgccg acgtgcgata cggcgggagt cttcggcccg tcggtccatg tcgtctcttc
      121 gctcgaagcg agcgaggcaa tcaagctcgc actcggccgg accgatgcga tcaaccggtc
      181 gctggtctcg ctcgatatct ggtcgatgga gctgcagcgg atcccggtcg gcggaccacg
      241 gccggattgc ccgacctgcc agcagcgcca gttcgacttc ctcgaccgga aatcgctcaa
      301 cctcgagacg acgctctgcg ggcacgatgc cgtgcaggtg atcatccagc cgccggtcaa
      361 actcgacctg gcggccctcg gcaagcggct cgaaccggcc ggcgatgtgc tggtcaaccg
      421 cttcctggtc cgcttcaccg acggggtcac cgggcacgag ttgaccatct tcccggacgg
      481 ccgcgcgatc gtgaagggga cgactgagcc tcgcgaagcg cgggcgattt atgatcgata
      541 tgtggggtcc tgaccaacga tgatctacct cgataatgcc gccacaagct ggccgaagcc
      601 ggaatccgta cgccaggcga tcgtcgaggc gctggatcaa tatggcgcga atccgggtcg
      661 atccagccac cagatggcga tccagaccgc cgagatgatc tatcgcacaa gagccgggct
      721 ggcccggctg gtcaatggcg actcgccgga gcggattgtc ttctgcacta acgcgaccga
      781 cgccctgaac ctggcgatct ttgggctggt ccggcccggc gacaaggtcg tgaccagctc
      841 gatggagcac aactcggtgc ggcggccgct gaggcggatg caggatctcg gcgccgaggt
      901 gatcacggtc cagacagagc gttccggcgt cgtcgatccg gctgcgatcc gcgcggtcgc
      961 tgatggggcg aagttgatcg cgatcacgca cggctcgaac gtgaacggcg cggtgcagcc
     1021 ggttgctgag atcggccgta ttgcacgcga ggtcggcgcc ctgctgctca tcgacgccgc
     1081 ccagacgatc ggggcgatgc cgatcgatgt ccgcgcgatg cagatcgatc tcctcgcctt
     1141 cccgggtcac aagtcgctgc tcggaccgag cggaaccggt ggcctctata ttggagagcg
     1201 ggttaacgtg gccgagttgg agccgctcca tgccggcggc acgggcttgc ggtcggaaga
     1261 tgacattcag ccgctcgagc tgccctatcg gtacgagtcg ggcacggtca atacgctcgg
     1321 gattgcgggg cttgcggccg ggctcaagca cgtcatgacc cttggcatcg aacagattca
     1381 ggcgcatcat acggagagca gagcgcggct cgcggcgggg atcgccgaat ccggcgcgac
     1441 gatgttcgag ccgccgatgg gcgccgccga tgtcgtctcg ttcgatatcc cgggttggca
     1501 gcctgccgat gtcggcagcg tgctcgacca ggcattcggg atcgcgtgtc gaacggggtt
     1561 gcactgcgct cctggagcgt gcgcaacgat cggcgcagga ccgaatggga caatacggtt
     1621 cagccccggc ctgacgacga cgggcgagga gatcgatgcc gcgatcgaag cggtcgcggc
     1681 aatcgcgagc gaagcgatgg tgcggtcatg aacgacccga acggcaatcc caggcggtcg
     1741 cgactcgaag acgaagtgct cgagatcctg gccaagacgg accgaaagcc gccgctgacg
     1801 gcgaaggcac gcgcctgggg ccgaactgcc cgagcgcaga tcctgaaatg gcgcgcccac
     1861 atcggttggc tcgacaccgc ctggggatgg ttcggcatcg cactcgccct cttcatcatc
     1921 ggcggctggg tcgcgaagga cagtgggctc ttgcagcaag tgatccagat tgccgggatc
     1981 gtggcgattg ccgtcgcggt gctccgactg gtccggccgg gttcggggca gggccgcaag
     2041 atgtggcgcg gacgcccgat ggacctcaag aagccgggcg tggagctggg cgacaacaag
     2101 ctcgacgatt ggcggaagcg aaggtaacca ggcgagcact cgagcagttc aaaacgtagc
     2161 gccggccccc tgtggccggc cgtttccttc gtagtggcgg ggctccgtac ccgcctcgac
     2221 tcgcccgaac acccagtctt ggtttcgcga ccggtcatca ccggagcgcc acatgcca
//
LOCUS       DATAOZ010000595         4324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2340879, whole genome shotgun sequence.
ACCESSION   DATAOZ010000595 DATAOZ010000000
VERSION     DATAOZ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340879"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..3240)
                     /locus_tag="VHR64_14965"
     CDS             complement(88..3240)
                     /locus_tag="VHR64_14965"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX3304186.1"
                     /translation="MTTDSSLDGLLRWRCIGPFRGGRVVAVAGSYSDRNTFYFGGCAG
                     GVWKTVDAGTYWRNVSDGFFTTSSVGAIAVAPSDSNVIYAGTGETTIRIDVSHGDGVY
                     KSSDEGRTWTNVGLRDTRHIGKIRVHPHDPDTVWVAALGHAFGPNKERGVFKSTDGGA
                     TWRQVLFVSEKAGAVDLTVDPANPRVLYAGVWEAYRNFWQISSGGPESGLWQSLDGGE
                     TWQNISGRFGLPKPIWGKVGVAASGATPGRAWALIEHKTEGGLYRTDDLGQTWEKVSE
                     DQNLLSRAWYYTHLTADPQDGNTVYVNNLDFHKSSDGGRTFEKIPTPHGDNHDLWIDP
                     LDNKRMIQGNDGGANVSLNGGYTFSSIYNQPTAQFYHLGTDNRDPYHIYGTQQDNSSL
                     AVPSAVNHGAITWGDCFVAGSGESGFITVRPDNPDVVYVGAIGSSPGGGNSLQRYDRK
                     RDQIRLITTWPEGDRGNGSKALKYRFAWTYPIVISPHDPDTLYIGGNFVFKSTDEGQS
                     WQQISPDLTRADPETLEPSGGPVNLDAVGAEVYATVFAFVESPHEAGELWAGSDDGLI
                     HISRNGGKDWADITPNQLPERSLISCIELSPFEPGVAYVAATRYKLDDYEPYLFVTRN
                     YGKTWRRINKGIPADDFTRVIRADPVQKGLLYAGTETGLYTSYDDGANWQRLQLNLPV
                     TPIHEILVKVSDLIAGTHGRSIWILDDLTPLRATAAGEGQATHLFAPRPTTRVLPGVD
                     WTDNVPGWTNYLGSVGSGFLTEVTPDGENRRTFLDAGENPPRGAIIAYSLAESPAEPI
                     SLVIRNAKGEEVRTFTSRKEDDPPKAKELRVPANAGGNRFVWDLRHAPITKIEGTDPM
                     AEEPIPGPFVPPGDYTVTLKIGDNELSQPFKVTKPATVDASESDLEAQYDLSLRVSRQ
                     LDRTVKTINRMRDVRSQLDGLAKRTKERDGAGEIPSAAEEIRERVLELEKLLAVPDLR
                     PGWADGINAGARLLEKLSGLPDVVHMGDYRPTDAAEEAYVDMTRRIEAVVADFDKLVQ
                     GELADLNAAAAAASIPVTIV"
     gene            complement(3324..>4324)
                     /locus_tag="VHR64_14970"
     CDS             complement(3324..>4324)
                     /locus_tag="VHR64_14970"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEX3304187.1"
                     /translation="LTNITREFPVNPLFIATGPGPYPTHREMHPAFYGCIDWHSCVEM
                     HWVIVRLLKLFPDAVPADKARATLSQLLSPENIAAEIRFFSDPNHRTIELPYGWGWLF
                     TLQHELETWDDSDGRNWAATIQPLPQHLIETMLEWLPKLTYPVRTGLHSNTAFGLARS
                     FNYATLLADRGDRRLLDAIHQHARRWFLADTDFPVRYEPSGSDFLSAGLSEAELMSLV
                     LEQAEFSEWLTAFLPGLADSEPHSLFHPAEVSDPTYGGIAHLHGLNLSRAAAFTKLAE
                     RLPLGDPRIKSMLAAAERHAMPSLPQVAGSHYNVEHWLAAYAVLLLSPPSDNRATT"
BASE COUNT          729 a         1295 c         1482 g          818 t
ORIGIN      
        1 cggccgcgat cgccacacct gtttcgatcc tcgcctgcaa cttcagccga tcgctatcga
       61 tcattccccg tttcctttcc tgctccatca cacaattgtg accggtattg atgctgcggc
      121 ggcagccgcg ttcagatcgg ccagctcgcc ctggaccagc ttgtcgaaat cggcgaccac
      181 ggcctcgatt cgacgagtca tatccacgta ggcctcttcc gctgcgtccg tcggtcggta
      241 gtctcccata tggacgacat ccggcaggcc cgacaacttt tcgagcagcc gggcgccggc
      301 gttgatgccg tccgcccagc cgggccgcag atcaggaaca gccagcagct tctcgagctc
      361 cagcacgcgc tcgcggatct cttcggcggc ggatggtatc tcgcccgctc cgtcgcgctc
      421 tttggttcgc ttggccagtc catccagttg cgagcgaaca tcccgcatgc ggttgatggt
      481 tttcacggtg cgatcgagct gtcggctgac gcggagcgag agatcgtact gggcctcgag
      541 atcggactcg gaggcgtcca ccgtcgccgg cttcgtcacc ttgaacggct ggctcagctc
      601 gttgtcccca atcttcagcg tgaccgtgta gtcgcccggt ggcacgaatg gtcccggaat
      661 gggctcttcc gccatcgggt cggttccctc gatcttcgtg atcggcgcgt gccggagatc
      721 ccagacgaac cggttgccgc cggcgtttgc gggtacgcga agctccttgg ccttgggcgg
      781 atcatcctcc ttgcgactgg tgaaggtgcg aacttcctcg cctttggcgt tcctgatcac
      841 gagcgagatc ggctcggccg gagattcagc gagcgagtac gcgatgatcg cgccacgggg
      901 cgggttctcg ccggcatcca ggaaggtccg gcggttttcg ccgtcgggcg tgacttcagt
      961 caagaagccg gatccgaccg agccgaggta attcgtccaa ccgggcacgt tgtcggtcca
     1021 gtcgacgccc ggaagcaccc gggtcgtggg acgcggggcg aagaggtgcg tcgcctggcc
     1081 ctcgccggcg gccgtcgcgc ggagcggggt gagatcgtcc aggatccaga ttgagcggcc
     1141 gtgcgtgccc gcgatcagat cgctcacctt gaccaggatc tcgtgaatcg gcgtgacggg
     1201 caggttgagc tggaggcgct gccagttcgc cccgtcatcg tacgaggtgt agagcccggt
     1261 ctcggtgccg gcataaagca ggcccttctg aaccggatcg gcgcggatca cccgagtgaa
     1321 atcgtcggcg gggatgccct tgttgatccg gcgccaggtc ttaccgtagt tgcgcgtgac
     1381 aaagagatac ggttcgtaat cgtcgagctt gtaccgggtc gcggcgacgt aggcgacgcc
     1441 cggctcgaac ggcgagagct cgatgcagct gatcagcgac cgctcgggca actgattggg
     1501 cgtgatgtct gcccagtctt tgccgccgtt tcggctgatg tggatcagac cgtcgtcgga
     1561 tcccgcccag agctcgccgg cctcgtgcgg cgattcgaca aaggcgaaga cggtcgcgta
     1621 gacctcggct cccacggcgt cgagattgac cggaccgccg ctcggctcca atgtctcggg
     1681 atcggcgcgg gtcagatccg gactgatctg ctgccaactc tgcccctcgt cggtcgactt
     1741 gaagacgaag ttgccgccga tgtagagcgt gtcaggatcg tgcggggaga tcacgatcgg
     1801 gtaggtccag gcgaagcggt atttgagggc cttggagccg ttcccgcgat cgccttcagg
     1861 ccaggtcgta atcaggcgaa tctggtcccg cttccggtcg tagcgctgca gcgagttgcc
     1921 gccgccgggc gaggagccga tcgcgccgac gtagacgacg tcgggattgt cgggccgcac
     1981 ggtgatgaag ccgctttcgc cgctcccggc cacgaagcag tcgccccagg taatcgcgcc
     2041 gtggttcacg gcactcggca cggcgagact cgagttgtct tgctgcgtcc cgtagatgtg
     2101 gtacggatcg cggttgtcgg tcccgaggtg atagaactgc gcagtcggct ggttgtagat
     2161 gctgctgaag gtatagccgc cgttcaggga gacgttcgcg ccgccgtcgt taccctggat
     2221 catgcgcttg ttatcgagcg gatcgatcca gagatcgtga ttgtcgccgt ggggcgtggg
     2281 aatcttctcg aacgtgcggc cgccgtcact gctcttgtgg aaatcgaggt tgttaacgta
     2341 aaccgtgttg ccgtcctggg gatcagcggt gagatgggtg taataccagg cgcgggagag
     2401 caggttttgg tcctctgaga ccttctccca cgtctggccg agatcgtcgg tccggtacag
     2461 cccgccctcg gtcttgtgct cgatcagagc ccaggcgcgg ccgggtgtgg cgccggacgc
     2521 agccacgccg accttacccc agatcggctt gggcaatccg aaccggccgg agatgttctg
     2581 ccaggtctcg ccgccatcca ggctctgcca tagcccgctc tccggtccac cgctgctgat
     2641 ttgccagaaa ttgcggtacg cctcccagac accggcatag agcacgcggg gattggcggg
     2701 atcgaccgtg agatcgaccg cgcccgcctt ctcactgacg aacagcacct gccgccaggt
     2761 cgcgccgcca tcggtcgact tgaagacgcc gcgctccttg ttgggaccga aggcgtgtcc
     2821 gagcgctgcc acccaaacgg tgtccggatc gtgcggatgg acacgaatct tgccgatgtg
     2881 ccgcgtatcg cgcaggccga cattcgtcca ggtccgtccc tcgtcgctgc tcttgtagac
     2941 gccgtcgccg tgggagacat cgatgcggat cgtcgtctca ccagtgcctg catagatgac
     3001 attcgaatcc gagggcgcca ccgcgatcgc cccgaccgac gaggtggtga agaagccatc
     3061 ggagacgttg cgccaatagg tgccggcgtc gacggttttc cagacaccgc cggcgcatcc
     3121 gccgaagtag aacgtgttcc ggtcggagta gctcccggct accgccacga cgcgtccgcc
     3181 gcggaatggg ccgatacagc gccatcgcaa aaggccgtcg agcgaggaat ccgtcgtcat
     3241 acaccaatcc ttgtccaaac gtgcgaacgc attgcgcgca acggtatcgc aatttgcgat
     3301 tgtgcgggaa ccatgaaatc ggctcaggtt gtagcgcggt tatcactcgg ggggctgagc
     3361 agcagcaccg catacgccgc cagccagtgt tcgacgttgt agtgggagcc ggcgacctgc
     3421 gggagggagg gcatcgcgtg gcgctcggcg gcggccagca tcgacttgat gcgcgggtcg
     3481 cccagtggca ggcgctcagc cagtttggtg aaggcggcgg cgcggctgag gttgaggccg
     3541 tggagatgag cgatgccgcc gtaggtggga tcggagacct cggccgggtg gaagagggag
     3601 tgcggctcgg agtcggcgag gcctggcagg aacgcagtaa gccactcgct gaattcagcc
     3661 tgttcgagga cgagcgacat gagctccgct tcgctgagac cagccgaaag gaaatcggat
     3721 ccggagggct cgtagcggac cggaaagtcg gtgtcggcca ggaaccagcg gcgggcgtgc
     3781 tgatggatcg cgtcgagcag gcggcgatct ccgcgatcgg ccagcagcgt ggcgtagttg
     3841 aatgagcggg cgaggccgaa ggcggtgttg gagtggagac ccgtgcggac gggataggtc
     3901 agcttgggca gccattcgag catggtttcg atcaagtgtt ggggcagagg ttggatcgtg
     3961 gcggcccaat tacgaccgtc cgagtcgtcc cacgtttcga gctcgtgctg gagcgtgaag
     4021 agccagcccc agccgtatgg cagctcgatc gtgcgatggt ttggatcgga gaagaagcgg
     4081 atctcggcgg cgatattctc cggactcagg agctgcgaga gggttgcgcg cgccttgtcg
     4141 gccggcactg catccgggaa gagcttaagg aggcggacta tgacccagtg catctcgaca
     4201 caggagtgcc agtcgatgca gccgtagaag gcggggtgca tctcgcgatg ggtagggtac
     4261 gggccggggc cggttgcgat gaagagggga ttgactggaa attcgcgggt gatgttagtc
     4321 aggg
//
LOCUS       DATAOZ010000596         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8392019, whole genome shotgun sequence.
ACCESSION   DATAOZ010000596 DATAOZ010000000
VERSION     DATAOZ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8392019"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1751)
                     /locus_tag="VHR64_14975"
     CDS             complement(<1..1751)
                     /locus_tag="VHR64_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3304188.1"
                     /translation="MVRLIPEFRGAIDPKSLNDINELIQARIDGGISRRQLVKRAAQI
                     GIAAPVVGVMLHATSDMAYGQPSHGRDRTIARMQGAATKEVTGPTQPAGTPQEGGTLV
                     TTSIDEPDTLNPWVTALVTTSDICCGVFNGLMRYDSNQQIIPDLAEEFEISTDGLIYT
                     FKLRQGVTFHDGAAFTAKDVDATWKAIMNPDFTAYSQLGWDHIKTIDMPDDFTVVLNT
                     TEVYAPFMAYISDPVSAAIVSGPVLDKGPDYFRGDFGRKPIGTGPFKVDEWKAKEQVS
                     LSRYDGYWGTKPKLEKIIYRVNPDDNTQLVQLQTGEAQMAASDGAIGSARLDEVLGFG
                     TVDVYERPNMAWSHLDLKHVDFLRITKVRQALDYATPTQQIIDSIMKGRVVRAFADQA
                     PITTVYATDITPRPYDLEKAKQLLTEAGLTQNGDGVWEGPTPTPSKDIDPNTDRGGPT
                     KEFKMEFWHLTGDSTTQQIAQVISQSWNSIGIKTETKSEDVSTIWGPDGYQFDPDTMT
                     ACMYSWFNSNDPDNMYYWHSSQIPPDPTGAGGNAIAYFFPLNFQAKVDELTVAGTKET
                     DPDKRKDIYIQLQHLIA"
     gene            complement(1956..>3485)
                     /locus_tag="VHR64_14980"
     CDS             complement(1956..>3485)
                     /locus_tag="VHR64_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3304189.1"
                     /translation="GVVGSIDTLNPYTTNLYAQGFDVLSGVMDGLVGYDSRQRLKPVL
                     AEGYTISDDGLTYTFTMRPNVLFQSGDKLTPQDVVASWQMIMNKELPVWSRLGWDKIA
                     SIDVPDDKTAVVKTAQLFAPFLSSISAGQFTTCAICPKSELDKGVDAFRERFDTKPIG
                     TGPFRIKSVRQKEIVLERYGKHWAGNPRLERITIRIYADYAAQLAALAAGEIQVANRT
                     GTPGSQNVDDSLTVAGASAFQFPGLTWGHLDLKNVSYLMDTRVRQALDYATPKADIIK
                     TVLNGYAQIGAADQAPGSWAYSQSLRPRPLDYEKASALMERAGFRLNSRNQLVKDGER
                     FEIDLWGEKGDSQAESILQMIAESWARIGLSCRVNLAPAAELWGPTGYQFTDRMTAGY
                     YRWANVNDPDDMFYWHSSQIPTHPGGAGGNVPAFFNEYSFQSEIDDLTSRAAAETDVV
                     LRKSLYLQIQDLLHEQVPAIFLFWDYNYSAAATTIGNYLPSAYTYMLWNAREWYLAQS
                     Q"
BASE COUNT          586 a         1062 c         1100 g          737 t
ORIGIN      
        1 tcggcaatca ggtgctggag ttggatgtaa atgtccttgc gcttgtcagg atctgtctcc
       61 tttgttcccg ccacagtcag ttcatcgacc ttggcctgga agttgagcgg gaagaagtag
      121 gcgatcgcgt tgccaccggc gccggtcgga tccggcggga tctggctgct atgccagtag
      181 tacatgttgt ccgggtcatt gctattgaac caggagtaca tgcaggcagt catcgtgtct
      241 gggtcgaact gatagccgtc cggaccccag atcgtcgaga cgtcttcgct cttggtctca
      301 gtcttgatgc cgatcgagtt ccacgactgc gagatgacct gggcgatctg ctgggtggtc
      361 gagtcacctg tcaggtgcca gaactccatc ttgaattcct tggtcgggcc gccccggtcc
      421 gtgttcggat cgatgtcctt cgacggcgtc ggcgtcggac cttcccagac tccgtcgccg
      481 ttttgggtca ggccggcttc ggtcaggagt tgcttggctt tttcgaggtc atacgggcgt
      541 ggagtaatgt ccgtcgcata gaccgtcgtg atcggtgcct gatcagcgaa ggcgcggacg
      601 acgcggccct tcatgatgct gtcgatgatc tgctgggtgg gcgtcgcata gtcgagcgcc
      661 tgccggacct tggtgatacg cagaaagtcg acgtgcttga gatcaaggtg actccaggcc
      721 atgttcggtc gctcgtagac gtcgaccgtg ccaaagccga gcacctcatc cagccgcgcc
      781 gatccaatcg cgccatcgct cgctgccatc tgggcctcgc ccgtttgcag ctggacgagc
      841 tgcgtattgt cgtcggggtt gacccggtaa atgatcttct caagcttcgg cttggtgccc
      901 cagtagccgt cgtatcgcga gagcgagact tgctccttgg ccttccactc gtcgaccttg
      961 aatgggccag tcccgattgg ctttcgccca aaatcgccgc ggaaataatc cggccccttg
     1021 tcgagaaccg gccccgagac gattgcagcc gaaaccgggt ccgagatgta cgccatgaac
     1081 ggggcgtaca cctcggtggt gttcagaacg acggtgaagt catccggcat gtcgatcgtc
     1141 ttgatgtggt cccatccaag ttgggaatat gcggtgaagt cgggattcat gatcgccttc
     1201 caggtggcgt cgacgtcttt ggcggtgaag gcggcaccgt cgtggaaggt cacgccctgg
     1261 cgcagcttga aggtgtagat caggccgtcg gtgctgatct cgaactcctc ggccaggtca
     1321 ggaatgattt gctggttgct gtcatatctc atcagaccgt tgaaaacgcc gcagcagatg
     1381 tccgatgttg tcaccagtgc ggttacccat ggattaagcg tgtccggctc atcgatcgag
     1441 gtcgtcacca gcgtgccgcc ctcttgcggc gtgcccgcgg gttgggtcgg tccggtcact
     1501 tccttggttg ccgcaccctg cattctggcg atggtgcggt cgcgcccgtg cgatggctgc
     1561 ccgtaagcca tatcggaggt cgcgtggagc atcacgccga cgaccggcgc ggcgatgccg
     1621 atctgcgccg cccgcttgac cagttgccgg cgcgagatgc cgccgtcgat ccgggcctgg
     1681 atcagctcat tgatgtcgtt gagcgatttc ggatcgatcg cgccacgaaa ctcgggaatc
     1741 aaacggacca tactgtttcc gctccttggg ttgccctccg gccgcccctc cgcgcccggg
     1801 taacgtcgct cccgcgcacg ctgcgcgggg aaaggtgctg agataggcgg tagcctacag
     1861 tgcaaatcca ttccgcgtcg aatggccctc ctcgcgtacg tatgctcgtt gcgcaagacc
     1921 gctcattgcc cgccattggt ttgattggcg cgtccctatt gcgactgcgc caaataccac
     1981 tcgcgggcgt tccacagcat gtaggtatag gcgctcggca ggtagttgcc gatggtggtg
     2041 gccgcggccg agtagttgta gtcccagaag aggaaaatgg ccggcacctg ctcgtgcagc
     2101 aggtcctgaa tctggagata gaggctcttg cgcagcacga cgtcggtctc ggccgccgcg
     2161 cgcgaggtca gatcatcaat ctcgctctgg aagctgtatt cattgaagaa agccggcaca
     2221 ttgccacccg ccccgcccgg gtgggtcggg atctgcgagc tgtgccagta gaacatgtcg
     2281 tccgggtcgt tcacattcgc ccagcggtag tagccggcgg tcatccgatc cgtgaactga
     2341 tacccggtcg gaccccagag ctcggccgcc ggtgcgagat tgacccggca ggagaggccg
     2401 atcctggccc agctctcagc gatcatctgc aggatcgact cggcctggga atcccccttc
     2461 tcaccccaga gatcgatctc gaatcgctcg ccgtccttga ccagctggtt gcgactgttc
     2521 agcctgaacc cggcccgctc catcagcgcg ctcgccttct cgtaatcgag tgggcgcggc
     2581 cggagcgatt ggctataggc ccagctcccc ggcgcctgat cagccgcgcc gatctgcgcg
     2641 tagccgttca gcacggtctt gatgatgtcc gccttgggcg tggcgtagtc cagcgcctgg
     2701 cgaacgcggg tatccattaa ataggaaacg ttcttgagat cgagatggcc ccaggtcaat
     2761 cccgggaact ggaaggcact cgcgcccgcg accgtcaggc tgtcatcgac gttctgcgac
     2821 ccgggcgtgc cggtccggtt cgcaacctgg atctcgccgg cggccagcgc cgcgagctgc
     2881 gccgcgtagt cggcatagat ccggatcgtg atccgctcca accgcgggtt gccggcccag
     2941 tgcttgccgt accgctcgag cacgatctcc ttctggcgaa cgctcttgat ccggaatggg
     3001 ccggtcccga tcggtttggt gtcgaagcgc tcgcggaagg catcgacccc cttgtccagc
     3061 tcactcttcg ggcagatcgc gcaggtcgtg aactggccgg cggagatgct ggagaggaac
     3121 ggcgcgaaga gctgggcggt cttgaccacg gcggtcttgt catccggaac gtcgatcgag
     3181 gcgatcttgt cccagccaag acgcgaccag accggcagct ccttgttcat gatcatctgc
     3241 catgaggcga cgacatcctg cggggtcagt ttgtcgccgc tctggaagag gacattgggc
     3301 cgcatggtga aggtgtaggt caggccgtcg tcggagatcg tgtacccctc ggccaggacc
     3361 ggcttcagtc gctggcggct gtcgtagccg accaacccgt ccatcacgcc cgacaacacg
     3421 tcgaatccct gtgcgtagag gttggtggta tacggattga gcgtatcgat cgaaccgacc
     3481 acgcc
//
LOCUS       DATAOZ010000597         6215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9178327, whole genome shotgun sequence.
ACCESSION   DATAOZ010000597 DATAOZ010000000
VERSION     DATAOZ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9178327"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VHR64_14985"
     CDS             <1..1033
                     /locus_tag="VHR64_14985"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX3304190.1"
                     /translation="YGNPVSEPYGAKSGYYSQAFEGAIFQFRPEFLWTETPSMTLEPI
                     GQEVLQSRTGQIRRDGKRQGGGGDRRASAWRTYGADSGVAQRAAANGGTFNADSGHSV
                     TGNFNDWYASHEGAWYLGAPLSQPVNERGMVVQYFEGAVLMRTGNDRVLVAPVVREQA
                     KTLRVQTAPVAQNDLPDYSETLLWTSENPNPLGDPNASGRKWIEISISQQTLWAYQGG
                     TLISSTLVSTGLDPNTTEEGLFHVRYKLPTQDMAGTTNADGEVVALGQEAADNAGVGT
                     EVGETPYVVQDVPNVMYFNLDAEALHGAYWHNNFGHKMSHGCVNLPLYFAAWLYGWAP
                     LGTEVWVHQ"
     gene            1208..2128
                     /locus_tag="VHR64_14990"
     CDS             1208..2128
                     /locus_tag="VHR64_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3304191.1"
                     /translation="MNETAESPLITTDAHLEPRIIAIANQKGGVGKTTTTVNLAALLA
                     DRGYRVLVVDADPQGNATSSLGVDKGELGETLYDAMLDPDGVQTLPLQEVRHGVDLYP
                     ATPDLAAVEVELVAQHSRERALARVLAATDAAYDICLIDCPPSLGLLTVNALTAADEV
                     LVPLQCEFLALEGLGQLLATIDLIKRGLNPELTVLGVLMTMYDARTRLSAHVVEEVRR
                     HMPNDLLSTIVPRAIRLAEAPSYGQVISEYDPDSRGAIAYEHLADELLARMGWPERRP
                     KNDTDLAGDGFVAAFDTGDALHDGHGFGAD"
     gene            2100..3659
                     /gene="guaB"
                     /locus_tag="VHR64_14995"
     CDS             2100..3659
                     /gene="guaB"
                     /locus_tag="VHR64_14995"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX3304192.1"
                     /translation="MTDTASAPTRRGANGKFSIGDQARAGKTMRQDKFAGLGLTFDDV
                     LLLPAASSILPAEVDTTTRIAADIVLRVPIMSAAMDTVTEGRMAIALARAGGIGVIHR
                     NLSIEDQVAEVDKVKRSESGMIVEPVTLTPDRAVRDAVAVMDHYHISGVPITEPDGKL
                     VGILTNRDLRFIDNIDQPIGGVMTKENLITAPVGTTLDEAAAILHRHRVEKLPVVDEK
                     GYLTGLITVKDIQKKIEFPNATKDSQGRLRVAAAVGVGSDAEERAAALVEDDVDLIVV
                     DTAHGHHESVLNMVRTLKSRFDVPIMAGNIATGDAAEALIEAGADAVKVGVGPGSICT
                     TRVVAGTGVPQITAVYDCVQAAAKHGIPVISDGGVQYSGDIAKAIAAGADAVMLGSLL
                     AGVDESPGEVVLYQGERFKEYRGMGSIGAMRARSFSKDRYFQEAVRGQEKLVPEGIEG
                     RVAYKGPLGNLVFQLVGGLRSAMGYTGSVDIASLKRDAQLIQITAAGLRESHPHDVFV
                     TKEAPNYRVSN"
     gene            complement(3792..5135)
                     /locus_tag="VHR64_15000"
     CDS             complement(3792..5135)
                     /locus_tag="VHR64_15000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3304193.1"
                     /translation="MATRSAGRRRGFPEDVTTPISTARTRGRARAYLPALAESWQLPA
                     AVFVVHWLLVQIPATLAWKYGELNGNSPPYGHEPPPVGGWVGNIVNPMRMWDGLWYRL
                     IAVEGYEGHTAKAAFWPLYPWTMRYAHEWFGISYEAAGYLIANICFAVALVLLYRLLS
                     LDFDRRIAAASIWALALFPTAFFFTAVYTESPFLVLLVGSLFAARLQRWWLAGVLGAL
                     AALTRSYGMFLVLPFAVLIWQQYGDSLRRQPAKLLAVGMPLLGPAFFSWRLNEIQGNW
                     QAWRDVQKEWNRYSAWPWETLRWAFQEHSPQNIALGFREGDGANWDWLHQLVDHPSWS
                     LITDKAWRLSTANSDTLELVCTLLFIVLAIVGLKVLPLYQTVYLWPGILVPLFQPSSV
                     HTLMSMPRFGLTLFPLFAVIGVLVAGRRIAPVLAVGSAVLLVLLTVQFSQWYWVS"
     gene            5253..6047
                     /locus_tag="VHR64_15005"
     CDS             5253..6047
                     /locus_tag="VHR64_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3304194.1"
                     /translation="MDLGLKGKVAVVAASSKGLGKATARTLAREGALVTVNGREAGTL
                     KATADEIRSETGADVIEIVGDMTNPADIDRLISETVEKRGGLDILVCNAGGPPSGAFS
                     NFPEDQPWLDAVNLNLMSTLRLMRAAIPHLEARGGGSITNIVSTSVKQPIGHLILSNT
                     ARTAVIGLAKSVSLEYAEKNIRVNNVCPGSTLTDRITSLAKTRAEKSGRSQEEILAED
                     AAAIPMGRLGTPQEFANVVVFMASPAAAYVTGTTLQVDGGNVRSLL"
BASE COUNT         1187 a         1980 c         2043 g         1005 t
ORIGIN      
        1 ctacggcaat ccggtatccg agccgtatgg cgccaagagc gggtattact cgcaggcatt
       61 cgagggcgcg atcttccagt tccggcccga atttctctgg accgagacgc cctcgatgac
      121 gctcgagccg attggtcaag aagtgctgca atcgcgcacc ggccaaatcc ggcgtgatgg
      181 caagcgccag ggcggtggtg gggaccggcg cgcctcggcg tggcgcacct acggagccga
      241 tagcggcgtc gcccagcgcg cggcggcgaa cggcggcacg ttcaatgccg actccggcca
      301 cagcgtcacg ggcaacttca acgactggta cgcctcgcat gagggtgcct ggtacctcgg
      361 tgcaccgctc agccagccgg tcaatgagcg cggcatggtc gtccagtact tcgaaggtgc
      421 cgtactgatg cgaaccggta atgaccgggt actcgtcgcg ccggtcgtgc gcgagcaggc
      481 caagaccctg cgggtgcaaa ccgctccggt ggcgcagaac gatctgccgg attactccga
      541 gacgctgctc tggacgtctg aaaacccgaa tccccttggc gatccgaatg cgtccggccg
      601 caagtggatc gagatcagta ttagccagca aaccctctgg gcataccagg gcggcacttt
      661 gatcagctcg acgttggtca gcaccggact tgatcccaac acgaccgaag agggcctgtt
      721 ccacgtcaga tacaagttac cgacgcagga catggctgga acgaccaacg cggatggcga
      781 ggtggtcgcg ctcggccagg aggccgcgga caatgccgga gtcggcaccg aggtcggcga
      841 gacgccatat gtggtgcagg acgtgccgaa cgtcatgtac ttcaacctcg atgccgaggc
      901 attgcatggc gcctattggc acaacaactt cgggcacaag atgagccacg gttgtgtcaa
      961 cctgccgctg tacttcgccg cctggctcta cggttgggcc ccactcggca ccgaagtctg
     1021 ggtccaccag taacgccctg cgattcggac taacccccga aacaccaaaa cctaaaaccc
     1081 aagacccaag accttccgtt ctggtgtcca cctcgacgag ggtgatagca tttggggatc
     1141 atgaacgcgc ctttgggggg tcgttcgaac gcggagtttc cccgatcgcc aacggtcgaa
     1201 gaagggcttg aacgagacag ccgagtcgcc gctgatcacc actgacgctc acctcgagcc
     1261 acgcatcatc gcgatcgcca accaaaaggg tggcgtgggc aagaccacga ccaccgtcaa
     1321 tctggccgcg ctccttgccg atcgcggcta tcgcgtgctg gtcgtcgatg cggacccgca
     1381 gggcaacgcc acgtcttcac ttggggtcga taaaggcgag cttggggaga cgctctatga
     1441 cgccatgctc gatccggacg gtgttcagac gctgccgctc caggaggtcc gccacggggt
     1501 tgatctctac ccggcgacac ccgatcttgc cgccgtcgag gtcgagctgg tcgcacagca
     1561 ttcgcgcgag cgagccctgg cgcgggttct ggcagcgacc gatgctgcgt acgacatctg
     1621 cctgattgat tgcccaccgt cgctgggtct actgacggtc aatgcgctga ccgccgccga
     1681 cgaggtcctg gtcccgctcc agtgcgagtt cctggcgctt gaggggctcg gtcagcttct
     1741 ggcgaccatc gacctgatca agcgcggcct caacccggag ctgactgtcc tcggcgtctt
     1801 gatgacgatg tacgacgccc ggacccgtct ctccgcgcat gtggtcgaag aggttcgccg
     1861 tcacatgccg aatgacctgc tgagcacaat cgtgccgcgc gcgatccgct tggccgaggc
     1921 cccgagctat gggcaggtca tctccgagta cgaccccgat tcccggggcg caatcgccta
     1981 cgagcatttg gccgacgagc tgttggcccg catgggctgg cccgagcgcc ggcccaaaaa
     2041 cgatacagat ctcgccggcg acggcttcgt cgccgctttc gacacaggag atgcactcca
     2101 tgacggacac ggcttcggcg ccgactaggc gcggcgccaa cggcaaattc tcgatcggcg
     2161 atcaggcccg cgccggcaag accatgcggc aggacaagtt cgccggcctt ggactgacct
     2221 tcgacgacgt cctgctcctc ccggccgcct cgtcgatcct gccggccgag gtcgacacca
     2281 cgaccaggat cgccgccgac atcgtgctgc gggtgccgat catgtccgcc gctatggaca
     2341 ccgtaactga gggtcgaatg gcgatcgcgc tggcccgggc cggtgggatc ggcgtcattc
     2401 accgcaacct ctcgatcgag gaccaggtcg ctgaggtcga caaggtcaag cgctccgagt
     2461 cgggcatgat tgtcgagccc gtcacgctga caccagatcg ggccgtgcgg gatgcggtcg
     2521 cggtcatgga ccactaccac atctccggcg tgccaatcac cgaaccggac ggcaagctgg
     2581 tcgggatcct gactaaccgc gatctccgct ttatcgacaa catcgatcag ccgatcggcg
     2641 gcgtgatgac aaaggagaac ctgatcaccg cgccggtcgg gaccacgctc gacgaggccg
     2701 ctgccatcct ccaccgccac cgggtcgaga agctaccggt ggtggacgag aagggctacc
     2761 tgaccggtct gatcactgtc aaggacatcc agaagaagat cgaattcccg aacgcgacca
     2821 aggattcgca gggtcgcctc cgggtggccg cggcggttgg tgtcggctcc gatgccgagg
     2881 agcgtgccgc ggcgctagtc gaggacgatg tcgatctgat cgtggtcgat accgcccacg
     2941 gccaccatga gtcagtcctg aacatggtgc ggacgctcaa gagccgcttc gacgtgccga
     3001 tcatggccgg caacatcgcc accggagacg ccgccgaagc attgatcgag gccggcgctg
     3061 acgcggtcaa ggtcggggtc gggccgggct cgatctgcac gacccgggtc gtggccggca
     3121 ccggcgtgcc gcagatcacc gctgtctacg actgcgtgca ggcggccgct aagcacggca
     3181 tcccggtcat cagcgacggc ggcgtccagt actccggcga catcgccaag gcgatcgcgg
     3241 ccggggcgga cgccgtcatg ctgggcagcc tgctggcggg tgtcgacgag agcccgggcg
     3301 aggtcgtcct ctaccagggc gagcgcttca aggagtaccg cggcatgggc tcgatcggcg
     3361 cgatgcgggc gcgatcattc tcgaaggacc gctacttcca ggaggccgtc cggggccagg
     3421 agaagctggt gccggagggg atcgaaggtc gggtcgccta caagggaccg ctcggcaacc
     3481 tggtcttcca gcttgtcggt ggcttgcggt cggccatggg ctacaccggc tcggtcgaca
     3541 tcgcgagtct taagcgcgac gcccagctga tccagatcac cgccgccggc ctccgcgaaa
     3601 gccacccgca cgacgtcttc gtaaccaaag aagccccgaa ctaccgcgtc agcaactagc
     3661 gggacttcgg acttcagtcg taagctgagt tgactgctgg ggttcgacgg caattcgtcg
     3721 aattgggccg acgaccagtt cgtcctcatc cgataactga cgtcggatgt tcgacgtctg
     3781 aagtctggcg tctacgatac ccagtaccac tggctgaact ggacggtcag caggacgagc
     3841 aggacggctg agccgactgc gaggaccggc gcaatccggc gtccggcgac cagcacccca
     3901 atgaccgcga agagcggaaa gagcgtcagg ccgaagcggg gcatgctcat cagggtgtgc
     3961 accgaagatg gttggaagag tgggaccagg atcccgggcc agaggtaaac cgtctgatac
     4021 agcggcagca cttttaagcc gacaatcgcc agaacaatga acagcagcgt gcagacgagc
     4081 tcgagcgtgt cgctgttggc ggtgctcaat cgccacgcct tgtcggtgat caggctccag
     4141 ctcggatggt cgaccaactg atggagccag tcccagttcg caccgtcacc ctcccggaat
     4201 ccaagggcta tgttctgcgg gctgtgctcc tggaacgccc agcgcaacgt ctcccagggc
     4261 caggcagagt agcggttcca ttccttctgg acatcgcgcc aggcttgcca gttgccctgg
     4321 atttcgttca gccgccagga aaagaaggct ggtccgagta gcggcatccc gacggcaaga
     4381 agcttggccg gttggcggcg gagcgagtcg ccgtattgct gccagatcag gacggcaaac
     4441 ggcagcacca ggaacatgcc gtaggagcgg gtcagggccg ccagcgcgcc gagtacgccg
     4501 gccagccacc agcgttggag ccgagctgcg aagagcgagc cgaccagcag caccaggaag
     4561 ggcgactcgg tgtagaccgc ggtgaagaag aatgcagtcg gaaagagcgc caacgcccag
     4621 atcgaggcgg cggcgatcct gcggtcgaaa tcgagcgaga gcaggcgata gagcagaacc
     4681 agcgcgaccg cgaagcagat gttcgcgatc aggtacccgg cggcctcgta cgagatgccg
     4741 aaccattcgt gcgcgtaccg catcgtccac gggtagagcg gccagaatgc ggccttggcg
     4801 gtgtgaccct catacccctc gaccgcgatc aggcggtacc agagaccgtc ccacatccgc
     4861 attgggttga cgatgttgcc gacccagccg ccgaccggcg gcggctcgtg tccatacggc
     4921 ggcgagtttc cgttgagctc gccgtacttc caggcgagtg tggccgggat ctggaccagc
     4981 agccagtgga cgacgaagac cgccgccggc agttgccagg attcggccag cgccggcagg
     5041 tatgcccgcg cgcgaccccg cgtccgggcg gtcgagatcg gagtcgtgac atcttcggga
     5101 aacccgcgtc gccgtccggc ggatcgtgtg gccatgtgca aagcataggg gggatacggg
     5161 caggatcgcc actggctgta acgagcgggg cacacacttc gggtacgcta ccggcaatat
     5221 tgcggttccg ataggcagga aggacagggc gaatggatct cggtctgaag ggaaaggtcg
     5281 cggtcgtggc cgcctcgagc aaggggctgg ggaaggcaac ggcgcggacg ctggcgcgcg
     5341 agggcgcgct ggtgacggtc aacggccgcg aggccggcac gctcaaggcg acagccgacg
     5401 agatccgctc cgagactggc gccgacgtga tcgagattgt cggcgacatg accaatccgg
     5461 ccgacattga tcgcctcatc agcgagaccg tcgagaagcg gggcgggctc gacattctcg
     5521 tctgcaacgc cggcggaccg ccgagtggcg ccttctcgaa cttccctgag gatcagccat
     5581 ggctggatgc ggtcaacctc aacctgatga gcaccctccg gttgatgcgt gccgcgatcc
     5641 cgcacctcga agcgcgcggt gggggcagca tcaccaacat cgtctcgacg tcggtcaagc
     5701 agccgatcgg acatttgatc ctctcgaaca cggcgcgaac ggccgtgatc ggcctcgcca
     5761 agtcggtctc gctcgagtac gcggagaaga acatccgggt caacaacgtc tgtcccggct
     5821 cgaccctgac cgatcggatc acgtcgctgg cgaagacgcg ggcggagaag tcgggcaggt
     5881 cgcaggagga gatcctggcc gaggatgcgg cggcgatccc gatgggccgt ctcggtacgc
     5941 cgcaggagtt tgcgaacgtc gtcgtcttca tggcctcgcc ggccgcagcc tacgtgaccg
     6001 gcacgacgct tcaggtcgac ggcggcaacg tccgcagcct gctgtaatcc cacaagacgg
     6061 gattgacctt gccggccaca gtgggccggg gctacgaatt gtccgaagtc cgaagtcccg
     6121 ccttacaggt cttcggctcc ttgagtcagg ttgaggtttt cgatgcggct ggagccgtcg
     6181 tcttcctcgg ccacgcgcca gacctcgttg aaggc
//
LOCUS       DATAOZ010000598         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7864481, whole genome shotgun sequence.
ACCESSION   DATAOZ010000598 DATAOZ010000000
VERSION     DATAOZ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7864481"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VHR64_15010"
     CDS             <1..472
                     /locus_tag="VHR64_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304195.1"
                     /translation="NGSGQTGTGETSGPSQPSQGTTGGQSNNAQTGAGAGSGSEGAGK
                     NQTSSGGDQKPGGSSGTSPDPNVGNGNGGGSGSTGQDNSTTQTHSSITLSRSPDATGM
                     QSGGSASSSSGSGSGAAAGPGSVSQGDVGTAGPDSNRVPKQYREIVEDYFSDKP"
     gene            472..1461
                     /locus_tag="VHR64_15015"
     CDS             472..1461
                     /locus_tag="VHR64_15015"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX3304196.1"
                     /translation="MATRFLTRLRPTPRTRSDRPIVTGDEPLLDQELMTSLQRLALSA
                     PRVQRGTFAGEHRSRRRGAAPEFADFKSYSPGDDIRRVDWNLYARFDELFVRLSEITT
                     DLTVHLLLDASASMDWRATPYTPTKYRFAQRVAAALAYVGLWHFDRIRVSPIAQGEWP
                     TFGPVQGRTQVPGLIAYLEDCRAGGDRTVAERLSAFAHREPTTGLLFILSDLLTDDLE
                     LLMTQCKTLRSKGWEIALLHVVDPAELLPEVMFADEDRSQPTTLIDSESGERVMVLPS
                     PASMQRYSGAVQAWLDDLTARCRAHGITYVRLATDQPVESAVLRLLNEQGLLK"
     gene            1458..3425
                     /locus_tag="VHR64_15020"
     CDS             1458..3425
                     /locus_tag="VHR64_15020"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX3304197.1"
                     /translation="MSGLSLLAPLGLAALVAVPVIVLLYMRTTTPTERRVPSTRFWLG
                     AQTTPTENRRFRLPPITPLFILHLLIAALIAFALAEPASAHVLSRFGSRTQPKHLILI
                     VDGSTSMQAAVDKLGVAGRTRFDLARNDVHDRLSSLGNGDVVSVLILGTHTTTYEASG
                     AVEIDQLRGQLDRLKAPGGRADFNAALRLCRDLLLPGLHDEIVVITDGAISVDPALVA
                     EIGAPIDLDLIKGNVSSNNLAITEINARGSTTIPGRQDLFIRVANFGDQTMTTTVDIS
                     VDNVSIDQREITLTAGTAQNLVETLPAGAETATATLSGSDALAADNRASVTLAGSGAT
                     GLRMLLVSDAPGALLRGLSALPGSQVQTLSLNQYLASTSLPAIDLIVFEGAAPSGSLP
                     SVPALVVNPGPGANANAGVMPAPVPTRLLAQDPVLNGVDLAGVSFGQVPKVSLSPDDV
                     EIAGADGGPLIYRTHLSNGEQADVLTFDIEASNLPVRVAFPILVSNLVSDLVAQQIPS
                     SLSVGDPITIAPHAGAASLEITDPKGTAHTMAVVTDETTGTTPLQSYEETGMSGPYWL
                     RELDASGNPITMATIMVNAGHPQESNLAPNPLLQDALTSNATEADQSRLRNQFEMWPL
                     VVAAVLGLLLIEWLLTLRQERRRPRAAGSRA"
     gene            3422..>4023
                     /locus_tag="VHR64_15025"
     CDS             3422..>4023
                     /locus_tag="VHR64_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX3304198.1"
                     /translation="MMPLAAIQMTFGRERALLLLVLIPLIGFISWRAVWRPKRLRRSV
                     LLLRLAMLALVIAALAEPMAARTGNASTTIMLVDQSTSVTSNSESGVNAWVKDALASA
                     GAGDNAAIVAFGGSADLAVPSSPASAISPDWTDSVDTTAIDPSFTNIESAVALARSLP
                     VGGNRRIVLVSDGSENLGSVQNQVAQAARDGVPIDVVKVAG"
BASE COUNT          643 a         1348 c         1356 g          676 t
ORIGIN      
        1 caacggatcc ggtcaaacgg gcacgggcga gacgtcgggc ccatcccagc cgtcgcaggg
       61 caccaccggc ggccaatcga ataacgcgca aaccggcgcc ggcgctgggt cgggatccga
      121 gggagccggc aagaaccaga cctccagtgg cggcgatcag aagcccggcg gcagctccgg
      181 cacttcgccc gatccgaacg tcggcaacgg caacggcggt gggtcaggct cgaccggcca
      241 ggacaactcg acgactcaga cgcactcctc gatcaccctc tcccgatcgc cggatgccac
      301 cgggatgcaa tcgggcggat ctgcctcgtc cagctccgga tcgggttctg gcgcggcagc
      361 cggcccgggc tcggtctcgc agggtgatgt cggcacggcg ggacctgact ccaaccgcgt
      421 tccgaagcag taccgcgaga tcgtcgaaga ctatttctcg gacaagccgt aatggctacc
      481 cggttcctga cccgcctccg cccgactccg cggacccgga gcgatcggcc gatcgtgacc
      541 ggcgatgagc cgctgctcga ccaggagctg atgacatcgc tgcaacggct ggcgttatcg
      601 gcgccgcgcg tccagcgcgg cacattcgcc ggcgagcatc gctcccggcg gcggggcgcg
      661 gcacccgagt tcgccgattt caagagctat agcccgggcg acgacatccg gcgcgtcgac
      721 tggaacctct acgcgcgctt cgacgagctt ttcgtccgat tgagcgagat tacgaccgat
      781 ctgaccgtcc atttgctgct cgacgcgagc gcatcgatgg attggcgcgc gactccgtac
      841 acgccgacca agtaccggtt tgcacagcga gtcgcggccg ctttggcgta cgtcggcctc
      901 tggcatttcg accggatccg ggtcagcccg attgcgcagg gtgagtggcc gacattcggt
      961 ccggtgcagg gccgcacgca ggtgccgggc ctgatcgcct acctcgagga ctgccgcgcg
     1021 ggtggggatc ggacggtggc cgagcgactg agcgcgtttg cgcatcgcga gccgacaacg
     1081 ggactgctgt tcatcctctc cgatctgctg accgacgacc tcgagctcct gatgacccag
     1141 tgcaagacgc tgcgaagcaa gggttgggag atcgcgctgc ttcatgtcgt ggacccggcc
     1201 gagctgttgc cggaggtgat gttcgccgac gaggatcgga gccagccgac gacgctgatc
     1261 gacagcgaat ccggcgagcg cgtgatggtg ctgccctccc ctgcctccat gcagcggtac
     1321 agcggcgcgg tccaggcgtg gctggatgat ctgacggccc ggtgtcgcgc gcacgggatc
     1381 acctacgtgc gactcgcgac cgatcagccg gtggaatcag cggtcctgcg gctgctcaac
     1441 gaacaggggc tcctgaaatg agcggcctgt cgttgctcgc cccgctgggt ttggccgcgc
     1501 tggtggcggt gccggtcatc gtgctgctct acatgcgcac gacgaccccg accgagcgac
     1561 gcgtgccgtc gacccggttc tggctcggcg cgcagacgac gccgaccgag aaccggcgct
     1621 tccggctacc gccgatcact cccctgttca tcctgcacct gttgatcgcg gcattgatcg
     1681 cgttcgccct ggccgagccg gcctcggccc acgtcctctc ccgcttcggc tcgcgcaccc
     1741 agccgaagca tctgatcctg atcgtcgacg gatccaccag catgcaggcg gccgtcgaca
     1801 agctcggcgt ggccgggcgg acgcgattcg acctggcccg gaatgacgtg cacgaccggc
     1861 tctcgagcct cgggaacggt gacgtggtca gcgtgctgat cctcggtacc cacacgacga
     1921 cctatgaggc gagtggggcc gtcgagatcg accagttgcg cggtcaactg gatcgcctca
     1981 aggcgccagg cgggcgggcc gacttcaacg ccgctctgcg attatgtcga gacctgctcc
     2041 tgcccgggtt gcatgacgaa atcgtggtca ttaccgatgg ggccatctcg gtcgatcccg
     2101 cgctcgtggc tgagatcggc gcgccgatcg acctggatct gatcaagggc aacgtctcgt
     2161 ccaacaatct ggccatcacc gagatcaatg cgcgcggctc gacgacgatc ccgggtcggc
     2221 aggatctgtt catccgggtc gccaatttcg gcgatcagac gatgacgacc acggtcgaca
     2281 tcagcgtgga caacgtctcg atcgatcagc gcgagatcac gctcacggcg gggaccgctc
     2341 agaacctcgt cgaaaccctg ccggccggtg cggagacggc aacggcgaca ctgagcggaa
     2401 gcgacgcgct ggccgctgat aaccgggctt ccgtcaccct tgccggctcc ggcgcaaccg
     2461 ggttgcggat gctgctcgtt tccgacgccc cgggcgctct tttgcggggg ctttcggctc
     2521 tgcccggctc gcaggtgcag acgctctcgc tgaaccagta cctggccagt acctcgctgc
     2581 cggcgattga cctgattgtc ttcgagggcg cggcgccatc cggctccttg ccgagtgtgc
     2641 cggcgctggt ggtcaatccc ggacccggcg cgaatgccaa cgccggggtg atgccggccc
     2701 cggtgccgac ccggttgctg gcccaggatc cggtcctgaa cggggtcgat ctggccggcg
     2761 tgtcgttcgg gcaggtgccg aaggtcagcc tgagtccgga cgatgtcgag atcgccgggg
     2821 cggacggcgg gccgttgatc taccggacgc acctttccaa cggcgagcaa gcagatgtgc
     2881 tgacgttcga tatcgaggcg tccaatctgc cggttcgggt ggccttcccg atcctggtct
     2941 cgaaccttgt ctcggatctc gtcgcgcagc agattccgtc cagcctctcg gtcggcgatc
     3001 cgatcacgat cgcgccgcac gctggagcgg cttcgctcga gatcaccgat ccgaaaggca
     3061 ctgcgcacac gatggcggtc gtgaccgacg agactaccgg gaccacgccg ctgcagtcgt
     3121 acgaggaaac ggggatgtcg ggtccgtact ggctgcggga gctggacgcg agcggcaacc
     3181 cgatcacgat ggcgacgatc atggtcaacg ccgggcatcc gcaggaatcg aacctcgcgc
     3241 ccaatcccct cctgcaggat gcgctgacgt ccaatgcaac cgaggcggac caatcccgat
     3301 tgcgcaacca gttcgaaatg tggccgctag tcgtggcggc ggtgcttggg ctgctcctga
     3361 tcgagtggct gctgacgctt cgccaggagc ggcgccgacc gcgggcggcg gggtcgcgcg
     3421 catgatgccg ctcgccgcca tccagatgac gttcggccgg gagcgcgcgc tgctgttgct
     3481 ggtgctcatt ccgctgatcg gcttcatctc ctggcgagcg gtttggcgtc cgaagcgatt
     3541 gcggcgctcg gtgctactgc tccggctggc gatgctggcg ctcgtgatcg cggcgctggc
     3601 cgagccgatg gccgcgcgca cggggaacgc atcgaccacg atcatgctcg tcgaccaatc
     3661 gaccagtgtg acgtcgaaca gcgagagcgg cgtcaatgcc tgggtcaagg atgcgctggc
     3721 cagcgcgggc gccggggaca acgcggccat cgtcgcgttc ggcgggtcgg ctgatctggc
     3781 cgtgccatcc agcccggcct ccgctatcag cccggactgg actgatagcg tcgacaccac
     3841 tgccatcgat ccgtccttca ccaatatcga gtcggcggtc gcgctggccc gatcgcttcc
     3901 agtgggcggg aatcggcgca tcgtgctcgt ctccgacgga tccgagaacc tgggttcggt
     3961 ccagaaccag gtggcgcagg cggcgcgcga cggtgtgccg atcgacgtgg tcaaggtggc
     4021 cgg
//
LOCUS       DATAOZ010000599         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5758335, whole genome shotgun sequence.
ACCESSION   DATAOZ010000599 DATAOZ010000000
VERSION     DATAOZ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5758335"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            573..1517
                     /locus_tag="VHR64_15030"
     CDS             573..1517
                     /locus_tag="VHR64_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3304199.1"
                     /translation="MLVIYFQWGSSGAFLSSQELSVVLRDTGRFGMIAVAEVMLMITG
                     EIDVSVGSTFSLAPYVMVLLSITLGVPVAVSAIVAVLLGVLIGVFNGIITVAFRVPSL
                     ITTIGTLFLLQGIVVSLYNSQPIIAPVEEPFNAVFGENLYGPADPVWSWHGLTAFTPF
                     LWTVVAVAVMALVLNRTTFGLHTIATGSNIVGAREIGVRTDRIKVYNFMIVGGLAAAA
                     GVINTIQFASADPLAGGPTLTLQAIAAAVIGGTSLLGGSGTAIGSLVGAFVVATLNNG
                     LVMLGAQATVSDIYLGAAIILAMMLNVQVDRIRARRRI"
     gene            1514..2341
                     /locus_tag="VHR64_15035"
     CDS             1514..2341
                     /locus_tag="VHR64_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX3304200.1"
                     /translation="MSHEIPPLDPSAAVILEMRHISKSFGAVTALVDVSIELRQGEVL
                     ALVGDNGAGKSTLIKILSGFHDPDGGTIVVHGKEVHFGSPRDARAHGIETVYQDLALI
                     KDLSIYHNMFLGREYHRRVLGLKLLDNRKMRLLARGYLDKLGITIPSVDSTVELLSGG
                     QRQSVAVARSIYSSPTILVLDEPLAALGVRESAHVLGLIQNLRRQRSVSIILIVHNYN
                     QVFEVCDRINFLHSGEVVLDASTSETSEEELIRIVKSGLGPQAIEAAANKVGDAVTS"
     gene            2380..2943
                     /locus_tag="VHR64_15040"
     CDS             2380..2943
                     /locus_tag="VHR64_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304201.1"
                     /translation="MPWNRPAGAVIVPLHFGTAMAPHTDGAAARHEPLRLTGGCHCGN
                     LELTFETRRQPGEMAVRACGCSFCRRHGVRTVSDPDGRVEFLVRDPLRLSRYRFALAT
                     AEFLICRTCGVYVGALMADAGSAYAIVNINALAMPGLFTQSALAVSYDQESTAERRGR
                     RRTRWTPAVMIEQSDRGRAAELGGPTR"
BASE COUNT          509 a         1084 c         1037 g          589 t
ORIGIN      
        1 cagcctggcc tacgtgacca aggacaacgt cgacctctac ctcggcaaga gccgcttcga
       61 gggcagcacc gacgccgagc cgagctaggt tcgggttgga gcgggccgct tcggtcaccc
      121 gttccgctcg cccggcgcga cggaaatgtg gcgtctgtcg cctctgtcag cttggggcgt
      181 gtcgccagag tgctggatcc tcagccaccg ctcatcccca ttcggatgaa tggggttgag
      241 cggcgcgtgc gcggcaatca tcaccgaacc ggctggtccc accccattct acgtgccgtc
      301 gcgtgaggcg ccgtggccaa gccattccga gcacatcgtg acaaagggct ttcggggcag
      361 ggccatcgag ctccggtgac caccgaaggc ccggatcgcg ctcgcggctc gcctcggtcc
      421 aggggccggt cgtaaggccc atggcacaaa cgacccgaac cggcgccgca gcgacacgca
      481 aggttggcat aagcgagccc gcaaccgcct tggagaggat tctggtcatc ctctcccgct
      541 accgggaagc cagcatcgcc gtcgtcgcgg tcgtgctcgt catctacttc cagtggggca
      601 gtagcggggc attcctgtcc tcgcaggaac tcagcgtcgt gctccgcgac accggccgct
      661 tcggcatgat cgccgtggcc gaagtcatgc tgatgatcac gggcgagatc gacgtctctg
      721 tcggcagcac cttctcgctc gcgccctacg tcatggtgct tctgtccatc actttgggcg
      781 tgccggtggc ggtgagcgcg atcgtcgccg tcctgctcgg ggtgctgatt ggcgtcttca
      841 acggcatcat caccgtggcg ttccgcgtgc cgtccctgat cactacgatc gggacgctgt
      901 tcctgctgca ggggatcgtc gtctcgctct acaacagcca gcccatcatc gccccggtgg
      961 aagagccctt caacgcggtc ttcggcgaaa atctctacgg cccggccgat cccgtctggt
     1021 cctggcacgg gctgaccgcg ttcacgccgt tcctgtggac ggtcgtggcg gtcgcggtga
     1081 tggctctggt cctgaacagg acgacctttg gcctgcacac catcgcgacc ggcagcaata
     1141 tcgtcggagc gcgcgagatc ggcgtgcgta ccgatcggat caaggtatat aatttcatga
     1201 ttgtcggcgg actcgccgcc gccgccggcg tcatcaacac catccagttc gcctctgctg
     1261 atccgctggc cggcggcccc actctcacgt tgcaagcgat cgccgcggcg gtgatcggag
     1321 gcacgtcgct gcttggcggc tccggcacgg cgatcggctc gctggtcggc gcgttcgtgg
     1381 tggcgaccct caacaacggc ctggtcatgc tcggcgccca ggcgaccgtg tccgacatct
     1441 acctgggcgc cgcgatcatc cttgccatga tgctgaacgt tcaggtggac aggatccgcg
     1501 cccggagacg gatatgagcc acgagatacc ccctctcgat ccgagcgcgg ccgtcattct
     1561 cgagatgcgt cacatcagca agagctttgg cgccgtcacc gcgctcgtcg acgtcagcat
     1621 cgagctccgt cagggcgaag tgctggcgct ggtcggcgac aatggtgcgg gcaagtccac
     1681 gctgatcaag attctctcgg gctttcacga tcccgacggc ggcacgatcg tggtccacgg
     1741 caaggaggtg cacttcggct cgccgcgcga tgcgcgcgcc cacggcatcg agacggtcta
     1801 tcaggacctg gccctgatca aggatctgag catctatcac aacatgttcc tcggccgcga
     1861 atatcaccgg cgcgtccttg gcttgaagct cctcgacaat cgcaagatgc gcctgctggc
     1921 gcgcggctac ctcgacaagc tcggaatcac gatcccgagc gtggacagca cggtcgagct
     1981 cctgtccggc ggccagcgcc agtcggttgc cgtggcccga tcgatctatt cgtcgccgac
     2041 catcctggtc ctcgacgagc cgctggcggc actcggggtg cgcgagagcg cgcacgtgct
     2101 cgggctgatc cagaaccttc gccgccagcg cagcgtctcg atcatcctga tcgtccacaa
     2161 ctacaaccag gtctttgagg tgtgcgatcg catcaacttc ctgcactccg gcgaggtcgt
     2221 gctcgatgcc tccacgtcgg agacgagcga ggaggaactg atccgcatcg tcaaatcggg
     2281 tctcggcccg caggcgatcg aggccgcggc gaacaaggtc ggggatgccg tcacgagttg
     2341 atacgaaagc ctgtcgtaaa cgaccgccca tggagggaga tgccatggaa tcgaccggcc
     2401 ggcgctgtta tcgtgccgct gcatttcggg accgcaatgg cgcctcatac tgacggggcg
     2461 gctgctcgac acgagccgct gcggctgacc ggcggctgcc attgcggcaa tctggagctg
     2521 accttcgaga cccgacggca gccgggcgaa atggccgtgc gggcctgtgg ctgctcgttc
     2581 tgccgccgcc acggcgtgcg cactgtttcc gacccggacg ggcgggtcga gttcttggtc
     2641 cgcgacccgc tgcggctgag ccgctatcgt ttcgctctcg ccaccgccga gtttctgatc
     2701 tgccggacct gcggcgtcta cgtcggcgcc ctgatggccg atgccggatc cgcctacgcg
     2761 atcgtcaaca tcaacgccct ggcgatgccc gggctgttca cgcagagcgc tctcgcggtc
     2821 agctacgacc aggaatcgac cgccgaacga cgggggaggc gacggacccg ctggacgccg
     2881 gcggtaatga tcgagcagtc tgatcgcggc cgcgccgccg agctcggcgg acctacccgt
     2941 tgaccaggcc cgggtttctg gcggagctcc ggatctacgg cggaaggtcg ctgggcgctc
     3001 atctgagagg tcctgcgacc gtgctctttc taacctgagc gacgaggagc tctaccagcg
     3061 cctgctcgac gccaagacag gcctggtctg cgccggcgcc atggcgccga gcgggtctga
     3121 ccctgcttgc cggcgtcttc ggcggagcct tgccggtctt ggggctgctg ccagcgggtc
     3181 ctgaccggtt gtagcgtcgt tccgcggtca atcccataa
//
LOCUS       DATAOZ010000600         4340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1284112, whole genome shotgun sequence.
ACCESSION   DATAOZ010000600 DATAOZ010000000
VERSION     DATAOZ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284112"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VHR64_15045"
     CDS             complement(<1..892)
                     /locus_tag="VHR64_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX3304202.1"
                     /translation="MLEPALAKAPERSVPFTTISGRPIERLYGPEDISHLDYQRDLNN
                     PGQFPYTRGIHPTGYRGKLWTMRQFAGFGTPEETNERYKTLLAAGGTGLSVAFDLPTL
                     MGRDPDHELSLGEVGKCGVSVVSLADMERLFDGISLRDITTSMTINSPAPMIFAMYLA
                     VAEKQGADWQALSGTIQNDILKEFIAQKEYIFPPRESMRLITDIFAFCSKEVPRWNTV
                     SVSGYHIREAGSTALQELAFTLRDGIEYIQYGIDAGLDADDFAPRISFFFNSHSDFFE
                     EIAKFRAARRLWASVMRDRFG"
     gene            2611..3861
                     /locus_tag="VHR64_15050"
     CDS             2611..3861
                     /locus_tag="VHR64_15050"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX3304203.1"
                     /translation="MEAGGSIFNAMLQEFDGAARLLGLDPGIWKILTHPKRQIIVSCP
                     IQMDNGEIEVFTGYRVQYNITLGPAKGGIRYHPDVSLDEVTALAAWMTWKCAVAHVPF
                     GGGKGGVVCDPTRLSRRELESLTRRYVAEIIDAIGPEKDVPAPDVNTNDQIMAWVMDT
                     YSMHVGHTSTAVVTGKPVEMGGSLGRREATGRGVMIVVREAAKHLGFDIKGARVAVQG
                     FGNVGSVSADLLSKIGAKIVAVTDWKGGVHNENGLDIEKMLDYARQHKTIDGFPGGDT
                     IENDQLFALDVEVLVPAALENQITMDNAPTISARVVAEGANGPTTPEAHKHLHERGIF
                     VIPDILANAGGVTTSYFEWVQDRHGYFWEEDEVNKRLETKMVEAFDDVLKTSMKYKTD
                     LRTAAYIVAISRVATVTKMRGMYA"
     gene            complement(4015..>4340)
                     /locus_tag="VHR64_15055"
     CDS             complement(4015..>4340)
                     /locus_tag="VHR64_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304204.1"
                     /translation="TIPELNAIKGNPDAIPTTEREFSRTDRLFVRADAYAPGGITPPV
                     TARLLNRGGQQMSDLKVQQAPGKPAEIELPLSPLAAGEYLIELNARSEGGSSAQELIA
                     FRVGR"
BASE COUNT          851 a         1350 c         1344 g          795 t
ORIGIN      
        1 cgccgaaccg gtcgcgcatc acgctggccc acagtctccg ggcggctcgg aacttggcaa
       61 tctcctcgaa aaagtcgctg tgcgagttga agaagaagga gatgcgcggg gcgaagtcgt
      121 cggcgtcgag accagcatcg atgccgtact gaatgtactc gatgccgtcc cgcagcgtga
      181 acgccagttc ctggagggcg gtcgatcccg cttcgcggat gtgatagccg ctcaccgaca
      241 cggtgttcca gcgcgggacc tccttggaac agaacgcaaa aatgtcggtg atcaggcgca
      301 tcgattcgcg cggcggaaag atgtactcct tctgggcgat gaattccttg aggatgtcgt
      361 tctgaatcgt ccccgaaagc gcctgccagt ccgcgccctg cttctcagcg accgccaggt
      421 acatcgcaaa gatcatcggg gcgggcgagt tgatggtcat cgatgtggtg atgtcgcgaa
      481 gcgagatgcc gtcgaacagc cgctccatat ccgcgagcga gacgacactg acgccacact
      541 tgccgacttc accgagcgaa agctcgtggt cgggatcgcg ccccatcaag gtcggcaggt
      601 cgaatgccac gctcaggccc gtaccgccgg ccgcgagcag cgtcttgtac cgctcgttcg
      661 tttcttcagg ggtcccgaac ccggcaaact gcctcatcgt ccagagcttg ccgcgataac
      721 cggtcgggtg gattccacgc gtgtacggga attgcccggg attgttcaga tcgcgctggt
      781 aatcgagatg cgaaatgtct tccgggccgt agagccgttc gattgggcgc ccggaaatcg
      841 tcgtaaaggg cacgctgcgc tcaggcgcct tcgccagcgc tggctcgagc acctcccgct
      901 cccatcgctg tcgcgccgtt gcagccgtcg cccctttcat aggtgattag attatagagg
      961 ctacggcgct ttcgcggttc gcgattcgcc ttttcgcgcc cggggctccg gtagccagac
     1021 ttcgcgtgaa acattcgcga agtctgactc tgaactcgat gggaactggg aactgagctg
     1081 gggagctgga acccaaacgg acggcaggca tgaacgcctg ccgtccgtta agaatcaatt
     1141 tgagaacgca cagacagcgt aaacacggaa cgtgaatgtc gcggcggtgt cctggctcag
     1201 tcgaaggacc acgcgccatg acgtttgggt cggaggaaac gacgcgagcg tatgcagcac
     1261 gagattggcg ttcgtttcgt atccaccacc caagacccgc ttgcctgtcg ggcaggcgac
     1321 ctcgagagtc gtttgagcgt tcgcgagcag gttcaccgtc accaacgccg tcgagacgac
     1381 ctgctgaccg ctgagtcccg gaggcccctg cggtccgatc ggaccgatgg ggccggttgc
     1441 accggtcgca ccggttgcac ccgtcgcacc cgtggcgccc tgaggtcccg caggaccttg
     1501 cggtccggcc gcaccctgcg gcccggcaga ccccgtcggt ccaacttcgc cctggggtcc
     1561 gactgccccc gtgtctccct tcggtcctgc aggtcccaca ggtcccgcag gtccagcagg
     1621 tccagcagcg ccggcgggac cagcaggtcc catatcgcct tgggcaccag caggtcctgt
     1681 cgcaccggca ggcccggcgg ggccgaccgg tccagcggga ccgacggatc cagctgggcc
     1741 aactggccca accgcaccag cggggccggc gggtccggcg ggaccagtag ctccggcggg
     1801 gccagccgcg cccgtcgcac cggtctctcc cttcggtcca gccgggcccg caggaccagc
     1861 tgcgcccgtc gcgcccgtat ctcctttcgg cccggcaggc cccgcagggc ccgtaggccc
     1921 ggtcggaccg gcaggtccga caggtccctg acccgccgtt ccgatataca tattgaaaaa
     1981 gccgatatcc ttgtcgccat ttccgcggct gaccgtcagg agatgcgtcc cgtcgggcat
     2041 cccgcctggc aggtaaagga cgacttgggt atcagtcgcg ctgatcaccg tcatcaggta
     2101 catctcgcac cagacttgag gcggctgcga cccgaagttc agcccatcaa ccgtgagcgt
     2161 ctggttgacg cgatcaacgg tcgtccccag gacggtcagc tgcttcgagc tgttgccatt
     2221 cccatttccg gtgttcttat tctgggcgta cgccaggtcc gttcccagaa tcacgagcaa
     2281 aagagccgtc aacaatgttc gactggaaag acgcataaag cctccagaca cgacggccaa
     2341 aagagcaagg gggaggccac ccctgcaata tcaattaact tgttcaatta caatgacttg
     2401 tcagaaatgt ttcagacttg aaactacggg tagtatgttc actttcgagc ccttgagcac
     2461 ggatttgatg gttattagaa gtactttata ggacaaattg gtcatcgcgg atcgatgcct
     2521 tgacttagtg caatcggcac agtagcttgg cctgtttggt caactgaata gcgccacgcg
     2581 aggtggtcgc tcggaagcgc agggagtcac atggaagcag gtggcagcat cttcaacgcg
     2641 atgctccagg agttcgatgg agccgcgcga ttactcggtc tcgaccccgg catctggaaa
     2701 atcctcaccc atcccaaacg ccaaattatc gtgtcctgcc cgattcagat ggacaacggc
     2761 gaaatcgaag tcttcaccgg ataccgcgtg cagtacaaca tcacgctcgg tccggcgaaa
     2821 ggcgggatcc gttatcaccc cgacgtgtcg ctcgacgagg tcacggcgct cgccgcgtgg
     2881 atgacctgga aatgtgcggt cgcacacgtg ccgttcggcg gtggtaaggg gggagtcgtc
     2941 tgcgatccca cgcggctgtc gaggcgcgag ctcgaatcgt tgacgcgccg gtacgtcgcg
     3001 gagatcatcg acgccatcgg ccccgagaaa gacgtccccg ctcccgacgt caacacgaac
     3061 gaccagatca tggcgtgggt gatggacacc tacagcatgc acgtcgggca cacctccacc
     3121 gcggtcgtca ccggcaaacc ggtggagatg gggggatcgc tggggcggcg agaagcgacc
     3181 ggccgcggcg tcatgattgt cgtccgcgaa gccgcgaagc acctggggtt cgatatcaag
     3241 ggcgcgcggg tcgccgtcca ggggttcggc aacgtcggtt cggtttcggc agatctgcta
     3301 tcgaagatcg gcgcgaagat cgtcgcggtc accgactgga aggggggcgt ccacaacgag
     3361 aacggtctcg acatcgagaa gatgctcgac tacgcccgcc agcacaagac gatcgacggg
     3421 ttccccggcg gcgacacgat cgaaaacgat cagctgttcg cgctcgatgt cgaagtgctc
     3481 gtacccgccg cgctcgagaa ccagatcacc atggacaacg cgccaacgat cagcgcgcgc
     3541 gtggtcgccg aaggggcaaa tggtccgaca acgccagagg cgcacaagca tctgcacgag
     3601 cgcggcatct tcgtcatccc ggacatcctc gccaacgccg gcggtgtcac gacgtcatac
     3661 ttcgaatggg tccaggatcg ccacggttac ttctgggagg aagacgaggt gaacaagcgt
     3721 ctcgaaacca agatggtcga ggcgttcgat gacgtattga agacgtcgat gaagtacaag
     3781 accgacctcc gcaccgccgc ctacatcgtg gcgatcagcc gcgtcgcgac ggtgacgaag
     3841 atgagaggca tgtatgcctg agtgtctgaa tgcggattgc ggatggcgga ttctttggtt
     3901 gccgattgtg gattaagact gccgattgtg gatttggcgc ctgtcactca atcgacaatc
     3961 tcaatcgtca atccgcaatc gcaaaatcgg cactccccaa tccgaaatcc gcattcatcg
     4021 accgactctg aacgcgatca gctcctgggc gctgcttcca ccttcactcc gtgcgttcag
     4081 ctcgatgagg tactcaccgg ccgcaagcgg ggacaggggc agttcgattt ccgccggctt
     4141 gccgggagcc tgctggacct tcaggtcgga catctgctga ccgccgcggt tgaggagccg
     4201 cgcggtcaca ggcggagtga tgccgcccgg cgcgtacgcg tcggcgcgga cgaacaagcg
     4261 atcggttcga ctgaactcac gctcggtggt tggaatcgcg tccgggttcc ctttgatcgc
     4321 gttgagttcc ggaatcgtcc
//
LOCUS       DATAOZ010000601         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6811608, whole genome shotgun sequence.
ACCESSION   DATAOZ010000601 DATAOZ010000000
VERSION     DATAOZ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6811608"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..290
                     /locus_tag="VHR64_15060"
     CDS             39..290
                     /locus_tag="VHR64_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304205.1"
                     /translation="MLYSVSATQGRITAIVSEAEREFFVVWTALDRIRAITPPEDLLH
                     LEADRAADTLLRLIREADCIDPSLMLDGTGTTQSLGPNP"
     gene            511..>2008
                     /locus_tag="VHR64_15065"
     CDS             511..>2008
                     /locus_tag="VHR64_15065"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX3304206.1"
                     /translation="MPWWHSLLFVAAVLFIALTVVVHPRSGASNPSTKTITYSVRDAG
                     SGASLQGATVTVGGQSLTTGVDGTVKMPVAPVEQDVIIQMIGYQTVYSSASSTTPKQQ
                     SVAMTMAPTPTATDVPPTPVPPTPTTVPLEKGDTFSGTVVDADGLSIAGAVVLLGSKW
                     VETDSDGAFSMPYQGGSDQARISASGYADRIVTAGKDLEISLERFMVKGIYLNGTAAG
                     EEAIVDTIIRLIDETELNAVVIDTKDSIVFYDSQVKFFRDAGAVDPTYDAKELVQKFH
                     DHGIYVIARQVAFKDPLVAEAHPELSIKDEKTGKPWRGWAGEAWVNPLKKGLYQPNVD
                     LAVETAGMGFDEIQYDYIRFPDGDLTGADFGKDYDNVDKRIGALTTLLTMTHDALRPL
                     GVKLSADVFGWMLLVDDDQGIGQRFPDIAAVVDYISPMVYPSHFPEGSIAVPGHPNDF
                     PYDVISISLDMGMAKIPGLERRMRPWIQDFTLPGMSEYGPEQIRDEINA"
BASE COUNT          359 a          694 c          583 g          372 t
ORIGIN      
        1 cccttttgtc ctatgaccac acaccgtagg ctgagtccat gttgtactca gtctcggcga
       61 cgcaaggtcg catcaccgcc atcgtctcgg aggccgagcg cgagttcttc gtggtctgga
      121 cggctctgga ccggattcgc gcgatcaccc cgccggaaga tctgctccac ctggaggctg
      181 atcgggccgc cgataccctg ctccggttga tccgtgaagc ggattgcatc gatccgtcgc
      241 tgatgctgga cggcactggc acgacccaat ccctcggccc taacccctga tcccgccccg
      301 atcgcccgat ccccgctcgt gacaaggtcg taatcggccg cgctttcgcc tattctttgc
      361 gcattcagca catgccgagc attggtctcc ggtgctgctc gcgatcgttg gtcgcccgct
      421 taacccggtg tcgtctgaat gcagcgtttt cgtccgaatt tgcgtgcgtc gtctcgccgt
      481 gcacgatcac gagaccgggc ctgggggcat gtgccgtggt ggcacagcct cctcttcgtc
      541 gctgccgtgc tcttcatcgc gctgacggtc gtcgttcacc cccgctccgg cgcatcgaac
      601 cccagcacca aaacgatcac ctactccgtg cgcgatgccg gttccggcgc gtcacttcag
      661 ggtgcgacgg tcacggtcgg cggccaatcc ctgaccaccg gagttgacgg gacggtcaag
      721 atgcctgtcg cgccggtcga gcaagatgtc atcatccaga tgattggcta ccagaccgtc
      781 tacagctcgg ctagctccac caccccaaag cagcaaagcg tcgccatgac catggcgcca
      841 accccgaccg cgaccgatgt cccgcccaca cccgtcccgc cgaccccaac caccgtccca
      901 ctcgagaagg gcgatacctt ctctggcact gtcgtcgacg ccgacggact gtcgattgct
      961 ggcgccgtcg tcctgcttgg ctcgaaatgg gtggaaactg attccgacgg cgccttctcg
     1021 atgccgtacc agggcggatc cgaccaggcc cgaatctcag cctcgggcta tgccgaccgg
     1081 atcgtgaccg ccggcaagga cctcgagatc tcgctcgagc ggttcatggt caagggcatc
     1141 tacctgaacg gcaccgccgc cggcgaggag gccattgtcg acacgatcat ccggctgatc
     1201 gacgagaccg agctcaacgc cgtcgtgatc gataccaagg acagcatcgt cttctacgac
     1261 tcgcaggtga agttcttccg cgacgccggc gcggtcgacc cgacctacga cgcgaaggag
     1321 ctagtccaga agttccacga ccacggcatc tacgtgatcg ctcgccaagt cgccttcaag
     1381 gacccgctgg tcgccgaggc gcatcccgag ctctcgatca aggacgagaa gaccggcaag
     1441 ccatggcgcg gctgggccgg cgaggcgtgg gtcaatccgc tcaagaaggg gctctaccag
     1501 ccgaacgtcg atctcgccgt cgagacagcc gggatgggct tcgacgagat ccagtacgac
     1561 tacatccgct tccctgatgg tgatctcacc ggagccgact tcgggaagga ttacgacaac
     1621 gtcgacaagc ggatcggcgc cctgaccacg ctgttgacca tgacccacga cgccttgcgt
     1681 ccgctcggtg tcaagctctc ggccgacgtc ttcggctgga tgctgcttgt cgacgacgat
     1741 caggggattg gccagcgctt cccggatatc gcggccgtgg tcgattacat cagcccgatg
     1801 gtctacccgt cgcacttccc ggagggatcg atcgccgttc ccggccaccc gaacgacttt
     1861 ccgtacgacg tgatctcgat cagcctcgat atgggcatgg ccaagatccc tggcctcgag
     1921 cggcggatgc gcccctggat ccaggacttc acgctccccg gcatgagtga gtacgggccg
     1981 gagcaaatcc gcgacgagat caacgcct
//
LOCUS       DATAOZ010000602         4208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2076135, whole genome shotgun sequence.
ACCESSION   DATAOZ010000602 DATAOZ010000000
VERSION     DATAOZ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076135"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="VHR64_15070"
     CDS             <1..1600
                     /locus_tag="VHR64_15070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX3304207.1"
                     /translation="SVVWDGIALLNAFAVATELLTQNRAAIDTLNVYPVPDGDTGTNM
                     GLTMQAALDEARSSSGPNPTVATVAQRIAYGSLLGARGNSGVILSQIYRGFAQGLAGL
                     DKVDGRDIAHALRSASDTAYQAVMKPVEGTMLTVIRVAAEHAEIAAQRDSSLIPVLDA
                     AFEGARVALANTPNQLETLRQAGVVDSGGQGVVVVLQGLLASAQGSSVEPGLVEVTSA
                     PIVVDGADELEAFGYCTNFLISGAGIPFEQVRADMAAMGQSAVIVGDDTHVKVHLHTE
                     NPGRALDFAIRYGSLSQIKIDNMDRQVSARSQQAATTPDGIAVLTVASGAGIAEAFRS
                     VGVAKVIDGGQTMNPSIEELLRAVNAAPGTEVILLPNNSNLLMAAQQIPTLAGKTVRV
                     VPTRSIAQGLAAVSAFRAGRPIDEVARAMDRAAKRIHSVEVSRADKDAAVNGVRVRRG
                     QYIGIVDDRLDFASDDLLETAIASIGRAITPESELVTIFAGDAVEAAIGEQLQRRLTS
                     ELADVDVELVEGGQPHYQFVIAVE"
     gene            1605..2459
                     /locus_tag="VHR64_15075"
     CDS             1605..2459
                     /locus_tag="VHR64_15075"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX3304208.1"
                     /translation="MPKIALVCDSTADLQDVDLVNLGVDMVPLNVHFGTELFRDYVDI
                     TPSEFLTRLATTPVMPRTSQPSPAAFQAVYESVAADCDAILVLTLSSKLSGTYQSAVL
                     GAKSANLSTPVRVLDSLSASIGLGLQVRRARSLIDSGLPIDEIVTTLEAERSQYHLIF
                     FADTLEYLQRGGRIGKASQLVGSILKIKPLLIVVDGEVEPYERTRSRSRAIEGLIEYA
                     RQFSTFDQLAILHDGTTPEDVTTIEQGLADLIAGSNVIVSQYGPIIATHVGPKALGLC
                     VFTGPPGH"
     gene            2565..>4208
                     /locus_tag="VHR64_15080"
     CDS             2565..>4208
                     /locus_tag="VHR64_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX3304209.1"
                     /translation="MRSEWRAGTSGRYLDKIRKAIEGLESQAPAQQTVEVIRSINGEL
                     NTYRASTVLGRQEQLKRTAERLKAVAPLLRPDEPPFGPIGTNPASLRAPKRSSRPVST
                     AVRRPDEPVTVLGGVGTAVAARLAQLRIETIEDLLRHTPRHHIDFSRPQAIGQVGRMF
                     SDESVTLAGRITELKSIPGKQRNRVEVRITDGTGWARITWFNPFVAKQLAVGDEIVVH
                     GPLDEFRGGLSLTGPEWERRDSATLARSRLIPVYALTQGLGQKQLRRLTRQAIDETRQ
                     TLTDPVPSSVLSELELVGLPFAIDQRHYPANRELLADAMRRLAFDDLFFLQLGLIQRK
                     RASDNLRGNSLASGEGGVPELIDRLPFQLTNAQHRAISDVSLDLGGSRVMQRLIQGDV
                     GSGKTVVAAAAALQATRAGFQTAVMAPTEILAGQLRTTLGRLYEDLGNDAPSVELLTG
                     STKKRDRTALLERLVNGEIDILVGTHAVIQDGVIFDRLGLTIVDEQHRFGVRQRGELP
                     SKGAVGPAHVLTMSATPIPRSLNLVLLGDIDVSVIDEMPP"
BASE COUNT          764 a         1388 c         1326 g          730 t
ORIGIN      
        1 tagcgtcgtc tgggacggga tcgctctgtt aaacgccttc gccgtggcca ccgagctgct
       61 cacgcagaat cgtgccgcga tcgacaccct gaacgtctat cccgttcccg acggcgatac
      121 ggggacgaat atgggcctga cgatgcaagc cgcgctcgac gaggcacgga gcagcagtgg
      181 gccaaacccc acggtagcca ccgttgcgca gcgcatcgct tacggttcct tgctcggtgc
      241 ccgcggcaat tccggcgtga tcctttcgca gatctaccgc ggcttcgctc agggtctggc
      301 cggtctcgac aaggtcgacg gccgggacat cgcccacgcc ttgcgtagcg cgtccgatac
      361 cgcgtaccag gcggtcatga agccggtcga aggaacgatg ctgaccgtaa tccgggttgc
      421 ggccgaacac gccgagatcg ctgcccagcg cgattcgtct ctgatccccg tgctcgacgc
      481 cgcgttcgaa ggtgctcgcg tagccctggc caacacgccg aaccagttgg agacacttcg
      541 tcaggccggc gtggtcgact ccggtggcca gggcgtcgtg gtcgtcttgc agggactgct
      601 cgcctccgcg cagggctcca gcgtggaacc aggtttggtc gaggtaactt cggcgccgat
      661 cgtcgtggac ggcgcagacg aacttgaggc gttcggttat tgtacgaact tcctaatctc
      721 cggcgccgga atccccttcg agcaggtgcg cgcagacatg gccgccatgg gccagtcggc
      781 cgttatcgtc ggagacgata cgcacgtcaa agttcacttg cacaccgaga acccgggccg
      841 ggcgctggat ttcgcgatca gatacggctc attgagccag atcaagatcg acaacatgga
      901 ccgccaggtg tcagcgcgat cgcagcaagc cgcgacaaca ccggacggca tcgcggtcct
      961 gacggtggcg agcggagccg ggatcgccga ggcgtttcga tcggtgggcg tcgccaaggt
     1021 catcgacggc ggccaaacca tgaatccgag catcgaagag ctcctgcgtg cggtcaacgc
     1081 cgcgcccggc accgaggtca tcctgctgcc aaacaacagc aacctcctaa tggcagccca
     1141 gcagatcccg acgttggcgg gcaagacggt gcgcgttgtg ccgacgcgat cgatcgccca
     1201 aggactggca gcagtctcgg cattccgggc cggccggccg atcgacgagg tggcgagggc
     1261 aatggaccgc gccgccaagc ggatccacag cgtcgaagtc tcccgcgccg acaaggacgc
     1321 cgcggtcaac ggcgtccgtg ttcgccgtgg ccaatacatc gggatcgtcg atgatcgcct
     1381 cgatttcgcc tccgacgacc tcctcgaaac cgcgatcgcg tcgatcggac gcgcgattac
     1441 acctgagagc gagctggtca caatctttgc gggcgacgcg gtcgaagcag cgatcggcga
     1501 gcagcttcag cggcggctca catcagaact ggccgatgtc gacgtcgaac tggtggaggg
     1561 cgggcaaccg cactaccagt tcgtgattgc ggtggagtag cgacgtgccg aagatcgcgt
     1621 tggtttgtga cagcaccgcc gacctccagg acgtcgacct cgtcaatctc ggggtcgata
     1681 tggtgccgct caacgtccat tttggtaccg agttgttccg cgactatgtc gacatcacgc
     1741 cgtcggagtt cctgacccgc ctggcaacga ccccagtgat gccgcgaacc tcgcagccct
     1801 caccggcggc attccaggct gtctacgagt cggtcgcggc ggactgcgac gcgatcctgg
     1861 tgctgaccct ctcgtcgaag ttgagcggca cataccaatc ggcggtgctc ggcgcgaaat
     1921 cggccaatct ttcgactcca gtccgcgtcc tggattcgct gagcgcatcg atcgggctcg
     1981 ggctgcaggt tcggcgcgcg cgttcgctga tcgattctgg actgccaatc gatgagatcg
     2041 tgaccacgct cgaggccgaa cgcagccagt accacctgat cttcttcgcc gacacgctcg
     2101 agtacctgca gcgcggcggt cggatcggca aggccagcca actcgtcggc tcgatcctca
     2161 agatcaagcc actactgatc gtcgtcgacg gcgaggtcga accctatgag cgtacccgta
     2221 gtcgatcacg tgccatcgag ggcctcattg aatacgcccg gcagttttcg actttcgatc
     2281 aattggcgat cctgcacgac ggcacgacac cagaagacgt cacgacgatc gagcagggac
     2341 tcgccgatct catcgccggg tcgaatgtga tcgtcagtca gtatggcccg atcattgcaa
     2401 ctcacgttgg gccgaaggcg ctcgggttgt gcgttttcac cgggccgccg ggtcattgat
     2461 tcaattcgcc gcgcattcag tacacttgat ctatggccac gcgcccacgc acaccaactg
     2521 aacgatccgg gacggagtca cctgtcgagc aggtgctcgt tttgatgcgc tccgaatggc
     2581 gtgccgggac atccggccgc tatctcgaca agattcgcaa ggcgatcgaa gggctcgagt
     2641 cccaggctcc ggcgcagcag acggtcgagg tgatccggtc gatcaacggc gagctcaaca
     2701 cgtatcgggc cagcacggtg ctcggcagac aagagcaact caaacggacg gctgagcggc
     2761 tcaaggcagt cgcgccattg ctgaggcccg acgagccgcc attcggcccg atcggaacca
     2821 acccggcctc gctccgcgcg cccaaacgat cctctcgacc tgtatcgacc gccgtgcgcc
     2881 gtcccgacga gccggttacg gtcctgggcg gtgtcggtac tgcggtcgcg gcgcgcctgg
     2941 cccagctccg gatcgagacg atcgaggatc tgctccggca tacgccgcgc catcacatcg
     3001 acttctcgcg cccccaagcg atcgggcaag ttggccgcat gttcagtgac gaaagtgtca
     3061 cgctggccgg ccggatcacc gagctcaagt cgatacccgg caagcagcgc aaccgggtcg
     3121 aggtgcggat taccgacggc accggctggg cccggatcac ctggttcaac ccgttcgtcg
     3181 ccaagcaact cgccgtcggc gacgagatcg tcgtgcacgg gccgctcgac gagttccggg
     3241 gcggcctctc gctaaccggt cccgagtggg agcgacgaga ctcggcgacg cttgctcgtt
     3301 cgcgcctgat cccggtctat gccctgaccc agggcctggg ccagaaacag ctccgccggc
     3361 tgactcgcca ggcaatcgac gagactcgcc agaccctgac cgatcccgtg ccttcgtcgg
     3421 tgctctctga gctagagctg gtcggactgc cgttcgcgat agaccagcgc cactacccgg
     3481 ccaaccggga actgctggcg gatgcaatgc ggcggcttgc gttcgacgac ctgttcttcc
     3541 tgcagctcgg cctgatccag cgcaagcggg cgtcggacaa cttgcgcggc aattcactcg
     3601 cctccggaga gggcggggtt cccgagctca tcgaccggct tccgttccaa ttgacgaacg
     3661 cgcaacaccg ggcgatttcg gacgtcagcc tggatctcgg cggctcgcgg gtgatgcagc
     3721 ggttgatcca gggcgatgtc ggcagcggca agacggttgt tgcggctgct gcggcattgc
     3781 aagcgacccg ggctggtttc cagaccgcgg tgatggctcc gaccgaaatc ctggcaggac
     3841 agctccgcac cacgctcggt cgcctctacg aagaccttgg caacgatgct ccaagtgtcg
     3901 agctcctgac tggcagcacg aagaagcgcg accggacagc cctgcttgag cggctcgtca
     3961 atggcgagat cgacatcctc gtcggcacgc acgcggtcat ccaggacggc gtcatcttcg
     4021 atcgactcgg cctgaccatc gtcgacgagc agcaccgctt cggcgtccgc cagcgcgggg
     4081 aactgcctag caagggagcg gtcggtccgg cccacgtgct gacgatgagc gcgacgccga
     4141 tcccgcgctc cctcaacctc gttttgctcg gcgatatcga cgtctcggtg atcgacgaga
     4201 tgccgccg
//
LOCUS       DATAOZ010000603         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3391495, whole genome shotgun sequence.
ACCESSION   DATAOZ010000603 DATAOZ010000000
VERSION     DATAOZ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3391495"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VHR64_15085"
     CDS             complement(<1..1298)
                     /locus_tag="VHR64_15085"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3304210.1"
                     /translation="MVSPSVGRTASALGIIMLCAASVHSQAAPAPTAPPKRIIIRAGR
                     LIDGAAENVRADQGILVEGDRIVAVGPYSDVSGRASGADRIDLSGMTVLPGLIDNHTH
                     VLLQGDITAADYDEQLLKESIPYRAIRATAAARTALLNGFTTIRDLETEGAMYADVDL
                     KTAIARSVVPGPRMFVATRAFSATGMYPLLGYSWELKMPEGVQVVDGPDEIRKAVREQ
                     VKYGADWIKVYADRKYYIASDGRLRSWANFTDEEFQAFVAEAHRLGRKVAVHAVGWDG
                     IDAALRAGVNTIEHGDGLTPDLIDRMVKQKVYWCPTIYVGVYVASGRGGPWPRMVELE
                     RQAFGTALKRGLLPYISYGTDAGGYAWTENQAKEFGYMVKYGMTPMQAIQSATVLGAE
                     LLEQSANFGSIEAGKFADLIVLDANPLVDLKNSAKIKYVM"
BASE COUNT          315 a          528 c          520 g          309 t
ORIGIN      
        1 ttcatgacgt acttgatctt cgctgagttt ttgagatcaa cgagcggatt cgcatcgaga
       61 acgatcagat cggcgaactt cccggcctcg atcgagccga aattcgcact ctgctccaac
      121 agctccgcac cgagcacggt agcggactga atcgcctgca tgggcgtcat cccgtacttc
      181 accatgtagc cgaactcttt tgcctggttc tcggtccacg cgtagccgcc cgcatcggtt
      241 ccgtacgaga tatagggcag cagtccgcgc ttgagggccg tgccgaaagc ctggcgctcg
      301 agctcgacca ttctaggcca cggaccaccc ctgccgctcg cgacatacac gcctacgtag
      361 atggtaggac accagtagac cttctgcttc accatgcggt cgatgagatc gggcgtgagg
      421 ccatcgccgt gctcgattgt attcaccccc gcccgcaacg ccgcatcgat gccatcccag
      481 ccaacggcat gaacggcaac tttccggccg aggcgatgag cctccgccac gaatgcctga
      541 aactcctcgt cggtgaaatt cgcccagctg cgtaacctgc cgtcggaggc gatgtaatac
      601 ttccggtccg cataaacctt gatccagtcg gcaccgtact tgacctgctc ccggactgcc
      661 ttgcggattt cgtccggtcc gtccacgacc tggacgccct ccggcatttt gagctcccac
      721 gaatatccca acaacgggta catcccggtg gcgctgaatg cccgggtggc cacgaacatg
      781 cgcggcccgg gcacgactga gcgcgcaatc gcggtcttga gatcgacatc ggcgtacatt
      841 gcgccctcgg tctcgagatc gcgaatggtg gtaaagccgt tgagtaatgc cgtgcgagcg
      901 gctgccgtag cgcggatagc ccggtatgga atggattcct tgagtagctg ctcgtcgtag
      961 tctgcggcgg taatgtcgcc ctgaagaagc acgtgggtgt ggttgtcgat cagaccgggc
     1021 aagacggtca tcccgctgag atcgatacgg tccgcgcccg atgcacggcc gctgacatca
     1081 gaatacggac cgacggcgac gatgcggtcg ccttcaacca ggatgccctg atctgcgcgg
     1141 acgttttcag cggcgccatc gatcaggcgc ccggcgcgga tgatgatgcg cttgggcgga
     1201 gcagtgggtg cgggagcggc ctgggagtgt accgacgccg cacacagcat gatgattccg
     1261 agggcactgg ctgttctgcc gaccgagggt gagaccatat gaactccggg ggaaggtacg
     1321 tcgccggacg tgcggtccgg gcagacggca gcaaattaca aactcgccat cgaatgggga
     1381 gcggatggga ccaccacggc ggctgctgca cactgtaatc gccggggtcg tagcggtgag
     1441 ctctcgccct gacaccctgg ccgaccccgc tttgcgatac ggatccggcc agctagactg
     1501 tgcccgcttc caggagacca gtcgaggcaa cgtggagacc tacaccgcgg gacgtgcgcg
     1561 ccgcgagtcg gtcgggcttg acggcgagtg gcgcttccga gcccagcccg gccgagccga
     1621 cagcgttcaa gtagaggcgt ggttcgacac actctcggtc tggcggcgca ct
//
LOCUS       DATAOZ010000604         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6023914, whole genome shotgun sequence.
ACCESSION   DATAOZ010000604 DATAOZ010000000
VERSION     DATAOZ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6023914"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VHR64_15090"
     CDS             <1..901
                     /locus_tag="VHR64_15090"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX3304211.1"
                     /translation="GSGEAMVVEADEYDYSFLWLRPAYAIITNIDYDHPDLFPDQSTY
                     DRAFVRFAQGMRPDGTLVVNGDDPGVQRVIDRFRSMPARVLTYGAGSGNDWRFDGRAV
                     TGPSGAEINLDLAVPGVHNRSNATAALVVMREMGIELPEAASALSSYRGVGRRFELKG
                     EADGVTVIDDYAHHPQEIAATIAAARERYPGRRIVAAFQPHTYSRTRALLDPFAQALG
                     AADRAIVLDIYPARETETLGVSSEDIVRRMNPERTVAGGTLAQAVDRLFRETRTGDVV
                     LTLGAGDVTLVGPKLLALLEDRS"
     gene            898..>1777
                     /gene="murB"
                     /locus_tag="VHR64_15095"
     CDS             898..>1777
                     /gene="murB"
                     /locus_tag="VHR64_15095"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX3304212.1"
                     /translation="MSSRTSNAPAIQVPGAPHLRVEQDSPLSRSTTWRIGGPADYLVR
                     VGDAADMVAAVRWGQDESLPITVIGGGSNLLVSDSGIRGLVIISKVSGEKATNLVDWQ
                     DLGDEVLLTVGAQAPLSWVGRFASEHGWAGMDWGVGLPGTIGGATVNNAGAHGTEQKD
                     HLESLRVLRLDGTIETHDRAWLDPTYRNTTIKAMSRPRDCVVLDVTMRLPKGDTAELV
                     RLADEHAAYRKATQPTGACGGSTFANPDGDFAGRLLEVAGLKGYAIGPVSFSGKHSNF
                     IINAGGGTAEQVRELIA"
BASE COUNT          301 a          543 c          619 g          314 t
ORIGIN      
        1 gggctcgggt gaggcgatgg tggtcgaggc cgatgaatat gactactcgt tcctctggct
       61 caggccggcg tacgcgatca tcaccaacat cgattacgac cacccggatc tgttcccgga
      121 tcagtcgacc tacgaccgcg ccttcgtccg gtttgcgcag gggatgcgtc cagacggcac
      181 gctcgtcgtg aatggtgacg accccggcgt gcagcgggtg atcgaccggt ttcgctcgat
      241 gccggcccgt gtgttgacgt acggcgcggg gtctggcaac gattggcgct tcgacgggag
      301 ggctgtaacc ggaccgtcgg gagccgaaat taatctggac ctcgcggtac ccggcgtcca
      361 caaccgttcg aacgccacgg cggctcttgt cgttatgcgc gaaatgggaa ttgagttacc
      421 ggaagcagct tcggcgttgt cgtcgtaccg cggagtcggt cgcaggttcg agctgaaggg
      481 cgaggcggac ggcgtgaccg tcatcgacga ttacgctcat catccccagg aaatcgccgc
      541 gaccatcgcc gccgcgcggg agcggtatcc tggccggcga atcgtcgccg ccttccagcc
      601 gcatacctac agccgcacga gggccctgct ggatccgttc gcacaggcac tgggtgcggc
      661 cgatcgagca atcgtcctgg acatttaccc ggcgcgcgag accgagaccc tcggtgtctc
      721 atccgaagac attgttcgcc ggatgaatcc ggaacgcacc gttgcgggcg gcacgctcgc
      781 gcaggcggtc gatcgcctct tccgggaaac tcggaccggc gacgtggtgc tgacgctcgg
      841 agcgggggat gttacgctgg tcggtcccaa gctgctggcg cttttggagg atcgatcttg
      901 agcagccgca cgagcaatgc accggcaatc caggtccccg gcgcgccgca tttgcgagtg
      961 gagcaggatt caccgctctc gcggagtacg acctggcgga tcggcggacc ggccgattac
     1021 ctggttcggg tcggagacgc tgctgacatg gtcgcggccg tgcgttgggg acaggacgag
     1081 agtctgccga ttacggtgat cggtggcggg tcgaacctgc tcgtgtctga cagcggtatt
     1141 cgcgggcttg tcatcatttc caaggtctca ggcgagaagg cgacgaacct ggtcgactgg
     1201 caggatctcg gagacgaggt tttgctcacg gtcggcgctc aggctccgtt gagctgggtc
     1261 ggccggttcg cgagcgaaca cggctgggcc gggatggact ggggtgtcgg tctgccgggg
     1321 acaatcggcg gtgcgacggt caacaacgcc ggcgcgcacg ggaccgagca gaaggaccac
     1381 ctcgaatcgt tgcgtgtcct ccgcctcgac ggaacgatcg agacgcacga tcgcgcctgg
     1441 ttggacccga cctaccgcaa caccacgatc aaggcgatgt ctcggccgcg cgattgcgtc
     1501 gtgctcgacg tcacgatgcg gctgccaaag ggcgatacgg ctgagctggt ccgactggcg
     1561 gacgagcacg ccgcctaccg caaggcgacg caaccgacgg gagcctgcgg cggctcgacg
     1621 ttcgctaatc cggacggcga ttttgccggt cgcctgctcg aggttgcggg cctgaaggga
     1681 tacgcgatcg gtccggtctc cttctccggg aagcacagca acttcatcat caacgcgggc
     1741 ggcggcacgg ccgagcaggt gcgggagttg atcgcgc
//
LOCUS       DATAOZ010000605         3448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5760394, whole genome shotgun sequence.
ACCESSION   DATAOZ010000605 DATAOZ010000000
VERSION     DATAOZ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5760394"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VHR64_15100"
     CDS             <1..1133
                     /locus_tag="VHR64_15100"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX3304213.1"
                     /translation="AAFAVLGFAVVVVASESMRVLVTGLVLAAVSVGATSYALRLTDV
                     ASAAELGLNARHPVLLMNLKSGGGKAEQFRLVELCRQGGIEPIVLHPGDNLRQLAEDA
                     VARGADLLGMAGGDGSQALVASVASRHGIPLVVVPAGTRNHFALDLGLDRDDVPGALD
                     AYQDGVDTVVDLAEVNGRVFVNNASMGVYARIVGSADYRDAKMQTAAAMLPDLLGPTA
                     KSLDLRFRLPSGEEAPTAQLLLVSNNPYQLARLRGGGMRERLDGGVLGIAFVRVDTAA
                     EAEKLAALDAIGRVRQFASWNDWTATEFEVRSAVPVEIGVDGEALTLEPPLHFVIRPR
                     ALTVRLPRAAARRSSAGLSHIMATPTLGALWQTVLGRPIGIR"
     gene            complement(1833..2387)
                     /gene="hemG"
                     /locus_tag="VHR64_15105"
     CDS             complement(1833..2387)
                     /gene="hemG"
                     /locus_tag="VHR64_15105"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008316.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HEX3304214.1"
                     /translation="MTKILIAYGTTEGQTARIADHLRELMRSRGLDADALNIKLAQGV
                     SVGGYDAVVVGGSIHMGKHQEDVVEFVRKNRADLEHLPSAFFSVSLAANGDLANAEAY
                     VENFVQQTGWRPSKVGLFSGALLYRRYGFLRRYMMKRIVRDKPGMSTDTSRDYIYTDW
                     DQVKRFADDFVKRFVSEDFAKIKT"
     gene            2967..3176
                     /locus_tag="VHR64_15110"
     CDS             2967..3176
                     /locus_tag="VHR64_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304215.1"
                     /translation="MPFDKCYHQLCDDLSNNNDSNINDQGLSEHSDAAVHAILTFAET
                     QSSVNGTGQGASKKAKEFKGHHKVR"
     gene            complement(3266..>3448)
                     /locus_tag="VHR64_15115"
     CDS             complement(3266..>3448)
                     /locus_tag="VHR64_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304216.1"
                     /translation="VPLAPLVAGVRLSVTALSYDGQFAVSLLIDENLSDLPVLAAGVR
                     AAFEGYLTPATRSADA"
BASE COUNT          632 a         1120 c         1072 g          624 t
ORIGIN      
        1 tcgcggcatt cgcagtgttg gggtttgccg tggtcgttgt ggccagcgag agcatgcgcg
       61 tgcttgtgac tgggctggtg ctcgcggcag tgtcggttgg agcgacgagc tacgcgctgc
      121 gcctgaccga cgtagccagc gccgccgagc tggggctaaa cgcccgccat ccggtcctgc
      181 ttatgaactt aaagtccggt ggcggcaagg ccgagcaatt ccgactggtc gaattgtgcc
      241 gacagggagg catcgagccg atagtgctgc acccaggcga caacctgcgc cagttggccg
      301 aggacgcggt cgcacgcggt gccgacctac tcggcatggc aggtggcgac ggctcgcagg
      361 cactcgtcgc gtcggtcgca agccggcacg gtatcccgct tgttgtcgtg cccgcaggaa
      421 cccgcaacca cttcgcgctt gatctgggac tcgaccgcga cgacgtacca ggtgcgctgg
      481 acgcgtacca ggacggcgtc gatacagtcg tcgatctcgc ggaggtcaac gggcgcgtct
      541 ttgtcaacaa cgcctcgatg ggtgtgtacg cgaggatcgt cgggtcggcg gactaccgag
      601 acgccaagat gcagactgct gccgctatgc tgccggacct gctcgggcca acagcaaaat
      661 cactcgatct acgctttagg ctgccgtctg gcgaggaagc gccaaccgcc cagctgctgc
      721 tggtctccaa caacccttac cagttggcac gcctgcgtgg cggaggtatg cgcgagcgtc
      781 tcgacggcgg cgtgctgggg atcgcctttg tccgggtcga cacggctgct gaggccgaga
      841 agctcgccgc gctcgacgcg atcggccgag tccggcaatt tgccagttgg aatgattgga
      901 ccgcgaccga gttcgaagtg cgctcggccg tgcctgtgga gatcggcgtt gacggcgagg
      961 ccctgacact ggagccgcca ctgcatttcg tcataaggcc tcgcgcgctg accgtccggc
     1021 tgccgcgcgc cgccgcgagg cggtcgtccg ccggcctctc acacattatg gcgacgccaa
     1081 cgctcggggc cctttggcaa acggtcctgg gccgaccgat cggtatccga tgaccagccc
     1141 caaaaactcc gagccggtcg aggagcaact tgctgaccgg ctcagtgcca ggcacctacg
     1201 gggccaatcg tcacgggtcg tacaggtcct ccgccagctc ggtcgcatgg accgagccgc
     1261 atatcggact gtggccgaca tgcacacacg gccgcttgac cgtccgcttc gtcggatatc
     1321 ggactttgcc gacttctcca agccctggtt tttgattgca ggagttctcg ctctcttcgg
     1381 cggagcgcgg ggacgacggg ccgccgtgac cggcgtggcc gcgttagggc tctcatcgtt
     1441 ccttgttaac caatcaatca agctgatcag tgagcgtcgg cgcccggacc gaaccgggca
     1501 cagcgtcccc cggagccgct gggttcggat gccacggtcg aactctttcc catccggcca
     1561 ttcagcatca gcggcagcat tcgctgtctc ggtgggggac ctgctgcccc cattcagggg
     1621 gccgctgcgg gcggccgcgg cgatcgtcgc attctcacgc gtctacaccg gcgtgcacta
     1681 tccggccgac atcgttgcgg gcgcggcgat cggcgccctg gtcggcagag ttacctctac
     1741 agcggcgcta cggcttccgg cttcatctcg acaacgcctg gaggatcggc ccgaggtcca
     1801 gacatgagcg agatcgtcgt cgaccctctc gactaggtct tgatcttggc gaagtcttcg
     1861 ctaacaaacc gcttcacgaa gtcgtcggcg aaccgcttta cctggtccca atcggtgtag
     1921 atgtaatccc gcgacgtgtc ggtgctcatg cccggcttgt cgcggacgat tctcttcatc
     1981 atgtagcgcc tgagaaatcc gtatcgccga tacagcagcg caccgctgaa gagaccaacc
     2041 ttgctgggac gccaaccggt ctgttgcaca aagttttcga cgtacgcctc ggcgttcgcg
     2101 aggtcgccgt tggcggccag acttaccgag aaaaaggctg aaggaagatg ctccaggtcg
     2161 gctcggttct tgcgaacgaa ttcgacgacg tcttcctggt gcttgcccat atgaatcgag
     2221 ccgccgacaa ccaccgcgtc gtagccaccc acagagacac cctgggcaag cttgatattc
     2281 aaggcatctg cgtccagacc ccggctgcgc atcagctcac gtagatgatc agcgatccgg
     2341 gcggtctgtc cttcggttgt cccgtacgca atcaagatct tggtcatttc gctccacctt
     2401 ccctaatcac tatccaatgt gccgcccttg ctgcggcggc cgcagagtcg aaggtcccga
     2461 ggtctacggg cgggcgacac acccgtcatt cgccgaccat tgcgcaactc gcggaatcgc
     2521 tgttagtgcg cccgggacag gtggcgccga agccctgaac gtcgtcggcg tcgctcgaga
     2581 tcaaaaggat cagcgaccgc tcggggaagt ttttatgcgc aagcgcgcct cattggcctc
     2641 tgcgctggcc ggcgccagtt cactattgat cacgtatccg gcggccgccg cctcgacgat
     2701 cgacaccgga gagctccgga atgccgtcac cgtggaaggc atcaagcagc atctcaccgc
     2761 acgtaacgcc attgcggatg cgaacccctt ccagggcata ccgacgcgcg acgggcacgc
     2821 ctggacacaa ggcgtctgtc gccttcgacg ggcgctcaga ctacgacgcc tttacggctg
     2881 ccggaatccc ggcaggcggc atcttcgccg gcacagaaga cgtcaagacg gcgcaatagg
     2941 tcgcgctcta cggcggggaa gccagcgtgc cgttcgataa gtgctaccac cagctctgcg
     3001 acgacctgag caacaacaat gacagcaaca tcaacgacca gggattgtct gagcactccg
     3061 atgccgccgt acacgcgatc ttgacctttg ccgagactca atcgtcggtc aatgggaccg
     3121 gtcagggagc gtccaagaag gctaaggagt ttaagggcca ccacaaggtg cgctaacgga
     3181 gctccgcaag caccatttga gctcggagct gcacccagcg cggcgttacc ggacagtgcg
     3241 ctgtcggcaa acaccgcgca gtgtgtcagg cgtccgctga tctggtggcc ggcgtgagat
     3301 aaccctcaaa cgccgcccgt acgccggccg ccagaaccgg cagatctgac aagttttcgt
     3361 cgataagcag ggacaccgca aactggccgt cgtatgacag cgccgtgaca ctcagccgca
     3421 caccggcaac cagcggcgcg agcggcac
//
LOCUS       DATAOZ010000606         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8917752, whole genome shotgun sequence.
ACCESSION   DATAOZ010000606 DATAOZ010000000
VERSION     DATAOZ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8917752"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VHR64_15120"
     CDS             complement(<1..652)
                     /locus_tag="VHR64_15120"
                     /inference="COORDINATES: protein motif:HMM:NF017073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HEX3304217.1"
                     /translation="MPYRARVLVRVNAVIGTVAVALAAVLAVVFLVIGPGEDKEKDRV
                     TAAATAFINGWVAGDFARAGAATNAPADAEAALNEAVDRLSVTATHIGRPAVSLGEDK
                     RTATARFRVQDTLAGLGAWDYETAAQLEKAADGGWKVIWSPRVIHPQLTAQTRLGRER
                     KVSERAPILDRDGKPLVTERPVITFGVWPSRLTDPDRAYEAIGKNLDVDLAALRSRV"
     gene            complement(733..1887)
                     /gene="rsgA"
                     /locus_tag="VHR64_15125"
     CDS             complement(733..1887)
                     /gene="rsgA"
                     /locus_tag="VHR64_15125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX3304218.1"
                     /translation="MIDLLSLGWDDGFAASYGPYDAPHNAPARVTRVDRGQVEAVRSA
                     GPVRAVLPKRLLAAAADDPTQYPCTGDWVVLAGAPAGGSRPNGAGPVRATEMIPDVDR
                     TSRPDPVRLTVEAVLPRRTAFVRAGVAKGVALGQVLAANVDVALIVEGLWPEPDFGRI
                     ERLLALAWESGAQPMVVLTKADLAADADQLRDDVAATAPGVDVYAISATTGAGMTALA
                     PYVVSGRSIALLGRSGAGKSTLVNALAGADVMATRAARSDHKGRHTTVHRELVMLPSG
                     GMIIDTPGLRSIGLWETEAGLDLVFADIDELAQRCRFGDCGHVTEPDCAVLAAIEDGT
                     LPVRRLESWRKLQREAAWIAARSDARLRQQRVAAWKRIHRDIRRSGQVRR"
BASE COUNT          389 a          794 c          735 g          353 t
ORIGIN      
        1 caacacgtga gcgcagcgca gccaggtcga catcgaggtt cttaccgatc gcctcgtagg
       61 cgcggtccgg atcggtgagc ctgctgggcc agacgccgaa tgtgatcact ggcctttcgg
      121 tgacgagcgg cttgccgtcc cggtcgagga tcggcgcccg ctcagaaact ttccgttccc
      181 tgcccagccg ggtttgcgcg gttagctgcg gatggatgac ccgaggggac cagatcacct
      241 tccagccgcc gtcggctgcc ttttccagct gcgctgccgt ctcgtagtcc caggctccaa
      301 ggccggccag ggtgtcctgt acgcggaaac gtgcagtcgc tgtccgcttg tcctcgccca
      361 gcgagacagc tggccggcca atgtgcgtcg cggtgacaga tagccggtcc acggcctcat
      421 tcagggctgc ctcggcatcc gccggcgcgt tggtcgcggc gcccgcacgg gcgaagtctc
      481 ccgcaaccca gccattgatg aaggcggtgg ctgcagccgt cactcgatcc ttctctttgt
      541 cctctcccgg cccgatgacc aggaagacga ccgcgaggac agcagccagt gcgaccgcga
      601 cggtcccgat gacggcgttg acccggacga gcacccgagc acggtacggc atgctccgct
      661 ttccggtcgt gatcgctgac agccggaccg cctgcaccct ggcgtccagc ggtcaggccg
      721 ttggcggcag ccctagcgcc gtacctgccc acttcggcga atatcgcggt ggatcctctt
      781 ccacgcggcg acccgctgct gcctcagtcg ggcatccgac cgcgcggcga tccacgctgc
      841 ctcccgctga agcttgcgcc agctctccag tcgccggact ggcagcgttc cgtcctcgat
      901 cgctgccaga accgcgcaat cgggctcggt gacatgtccg cagtcaccga atcggcacct
      961 ttgtgccagc tcgtcgatat cagcgaagac caaatcgaga ccggcttcgg tctcccacag
     1021 accaatcgat cgcagcccgg gagtatcgat gatcatgccg cctgacggca gcatgacgag
     1081 ctctcggtgg accgtcgtgt gcctgccctt gtggtcgctg cgcgccgccc gggtagccat
     1141 gacgtccgca cccgccaacg cattgaccag ggtggacttt ccagcgcctg aacggccaag
     1201 cagcgcgatc gagcgcccgg acacgacgta cggcgcgagc gccgtcatgc cagcgccggt
     1261 cgtggcggag atcgcataga catcgactcc cggagcggtg gcagccacgt cgtctcgcag
     1321 ctgatccgcg tcggccgcca aatccgcctt ggtgaggacg accatcggct gagcgccgct
     1381 ttcccacgcg agcgcaagca gccgctcgat gcggccgaag tcgggctcgg gccagagacc
     1441 ttcaacgatc agcgcgacgt cgacgttcgc ggcgagcacc tggccgaggg caacaccctt
     1501 cgccaccccc gcacggacga acgccgtacg ccgcggcaac accgcctcca cggtgagccg
     1561 gaccgggtcc ggacggctgg ttcgatcgac gtcggggatc atctcggtcg cccggactgg
     1621 accagcgcca ttcggacgac tgccaccggc tggcgcaccg gcaaggacga cccaatctcc
     1681 cgtgcacgga tactgcgtcg gatcatcggc ggcagcggcg agcagacgtt tggggaggac
     1741 ggcgcggacc ggacctgccg accggaccgc ctcgacctga ccacggtcga cgcgagtgac
     1801 ccgcgccggg gcgttgtgtg gcgcatcgta cgggccgtac gacgcggcaa agccgtcgtc
     1861 ccagccaagg gacaacagat caatcatgga agacaaggtt ggagtgaacc cttcggatca
     1921 gcggctgtcc gccgcgatcg cgcggatccg gattgacgcg ccgagtgcgc gtccggacag
     1981 gtccagcggg atcaggcggt tgcagcccag gaagcaagac gggtgggcga agataggcag
     2041 aagacaacga tggccatgag acccacccct tccgagatcg cgagggtcgc ggacgccgac
     2101 aagtgtacgc atcgccgacc cacagccata gcggttttgc ggtccgcgta agtcctcaac
     2161 ccagccatcc ctcggcgatc aagcctcgaa ccggtactcc ttgctctgga accggtcggt
     2221 tgcgtgacct cagcgaggag gcgccggcgt cggaaagcgc cgctcgcgat c
//
LOCUS       DATAOZ010000607         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7868047, whole genome shotgun sequence.
ACCESSION   DATAOZ010000607 DATAOZ010000000
VERSION     DATAOZ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7868047"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VHR64_15130"
     CDS             <1..240
                     /locus_tag="VHR64_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304219.1"
                     /translation="SVAAASSPTAATALASPTAFTTEDPTNGNPLSPQSDFDSRALGI
                     SVGAGLIAMAAIVGFWFAIRPRNPRFGPRSGRNRE"
     gene            244..>1999
                     /locus_tag="VHR64_15135"
     CDS             244..>1999
                     /locus_tag="VHR64_15135"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX3304220.1"
                     /translation="MESADRRALICLLGLLLGLIIVGGRPDQAAAHATLGSSDPPANE
                     IISKSPGRVDLRFTEPVEETYTKIILVDQNGAEIPGTSTVVDQSDPSIARMVIPDDLP
                     RGTYSVVWRTLSAADGHRFSGYFAFTIGSSSDVQTVIPPTFSDSGGPPFWVEVLSRWF
                     AFLTMSLLTGLWLTWVAVIRPALSPVWQVGPTVVTRVRRFALIAGLLYLAGSVGALLV
                     QAGGQADGDLLGAINDTLVDTRWGRIWTLRLIFGALTTVLFLAAAWWWPRRRLLTFGG
                     LFALSAFIPLPHALISHASAQDAGRSEAIFADYIHLLAMGLWIGGLGVIAATLTASGD
                     LGPDGRKAFLVRLMPRFSAIALTCWVALGLTGLYAGYLQIGSWAGLRDTQYGRALIYK
                     LAILVLVLIVASINLLVVTRKIRTASGESAPGWWHRFAGLIALELIGVLAVLIFVGRM
                     TSMEPSRPVLAERENQQSIAFTIGERSATLSMAPGTAGPNHIRLDISGDPLPAKTEAS
                     ILLAPPVDMAGQKSVDLERTTGNTFEAHSAEMSVAGDWGMTLTVSEVGAFQWSAELTH
                     TAAQRKPGARSISIPTWTL"
BASE COUNT          292 a          676 c          654 g          377 t
ORIGIN      
        1 agcgtggcag ccgcatcgtc gccgaccgcg gcaacggcgc tggcgagtcc gactgcgttc
       61 acgaccgagg atccgaccaa cgggaacccg cttagtccgc agagcgactt cgacagccga
      121 gccctcggga tcagtgtcgg cgccgggctg atcgcgatgg cggcgatcgt cggattctgg
      181 ttcgcgatcc ggccccggaa cccgagattc ggaccccgct cgggccgaaa tcgggaatag
      241 cccatggaga gtgccgaccg acgcgcgctg atctgcctgc tggggcttct cctcggcctg
      301 atcatcgtcg gcggtcgacc ggatcaagcc gcggcacacg caacgctcgg cagctctgat
      361 cctccggcca acgagatcat ctcgaaatcg cccggccggg tcgatctccg cttcacggag
      421 ccggtcgagg agacctacac gaagatcatc ctcgtcgacc agaacggcgc cgagattcca
      481 ggcacgtcga cggttgtcga ccagagtgat ccgtccatcg cccggatggt gatcccggac
      541 gacctcccgc gcggcaccta ctcggtcgtc tggcggacgc tctccgctgc cgacgggcac
      601 cgcttctccg gctacttcgc tttcacgatc ggctcttcgt ccgatgtcca gaccgtcatc
      661 ccgccgacct tcagcgactc cggcggccca cccttctggg tcgaggtcct gtcgcgatgg
      721 ttcgctttcc tgacgatgag cctgctgacc ggtctctggc tgacctgggt ggcggtgatc
      781 cggcccgcgt tgtcgccggt ttggcaggtc gggccgacgg tcgtcacccg tgtccgtcga
      841 ttcgccctga tcgccggctt gctgtacctg gccggatcgg tgggtgcact gttggtccag
      901 gctggtggtc aggccgatgg cgacctgctc ggcgcgatca acgacaccct ggtcgatacc
      961 cgttggggcc ggatctggac gctgcggctc atcttcggtg cattgacgac ggtcctcttc
     1021 cttgcggctg cgtggtggtg gccgcgccgg cggctgctga ccttcggtgg acttttcgcc
     1081 ttgagcgcgt tcattccgct gccgcacgcg ctgatctcgc acgcttcggc ccaggacgcc
     1141 ggtcgcagcg aggcgatctt cgccgactac attcacctgc tggcgatggg actctggatc
     1201 ggcgggctcg gcgtgatcgc cgcgaccttg accgcttccg gcgatctcgg tccggatgga
     1261 cgcaaagcct tcctcgtccg ccttatgcct cgcttctcgg ccattgcact gacctgctgg
     1321 gtggcccttg gtctgaccgg tctctacgcc ggatatctcc agatcggctc gtgggctggt
     1381 ttgcgcgaca cgcaatacgg ccgcgcgctg atctacaagc tggcgatcct ggtgctcgtg
     1441 ctgatcgtgg cgtcgatcaa cctgctggtc gtcacgcgga agattcgcac cgcctctggc
     1501 gagagtgcgc ccggatggtg gcaccggttc gcgggactga ttgcactgga gctgatcggc
     1561 gtcctggcgg tcctgatctt cgtcgggcgg atgaccagca tggagccgtc ccgaccagtc
     1621 ctggccgagc gcgagaacca gcagagcatc gcgttcacga tcggcgagcg atcggcgacg
     1681 ctctcgatgg cgccgggcac ggccggtccg aatcacatcc ggctcgatat ctcgggcgat
     1741 ccgctgccgg cgaagaccga agccagtatc ctgctggctc cgccggtcga catggcgggt
     1801 cagaagtccg tcgacctgga acggaccacc ggcaacacct tcgaggcgca ttcggccgag
     1861 atgagtgtcg ccggcgattg ggggatgacg ctgaccgtca gcgaggtcgg cgcattccaa
     1921 tggtccgccg agctgacgca tacggccgca cagcgaaagc cgggcgcgcg ctcgatcagc
     1981 atcccgacct ggacgctga
//
LOCUS       DATAOZ010000608        10865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7868082, whole genome shotgun sequence.
ACCESSION   DATAOZ010000608 DATAOZ010000000
VERSION     DATAOZ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7868082"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VHR64_15140"
     CDS             complement(<1..442)
                     /locus_tag="VHR64_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304221.1"
                     /translation="MTDREQATLPENDAESDERDVETAETAPAADDLTAQPVDTATEA
                     ADETDGPAPEPDVEPESLQRAEATGALVLGVLAPDDAAAAAQEIARSATLQAEVATLL
                     PVADILLSLYQQAQPAPSATATTAAIAAPPAEPARRAERPRAEPI"
     gene            complement(439..735)
                     /locus_tag="VHR64_15145"
     CDS             complement(439..735)
                     /locus_tag="VHR64_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304222.1"
                     /translation="MSSLPSIAVAPGVEISPAADDPLVARRAEITRELLREGIRQLSP
                     LERDALRLATRERLSVPDAATQLEVDPHVVETSLRSGLLSLRQSLIEQLGESER"
     gene            921..1088
                     /gene="rpmH"
                     /locus_tag="VHR64_15150"
     CDS             921..1088
                     /gene="rpmH"
                     /locus_tag="VHR64_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX3304223.1"
                     /translation="MPKRTYQPKRIPRLREHGFLSRMATRNGRAILTARRRKGRHKLS
                     VSDEGKFTQNK"
     gene            1152..1505
                     /gene="rnpA"
                     /locus_tag="VHR64_15155"
     CDS             1152..1505
                     /gene="rnpA"
                     /locus_tag="VHR64_15155"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX3304224.1"
                     /translation="MDRSLRLHESYDVKQTRSRGKSGASGPLVARVLPNALDPARNRY
                     TVVAGKKVGKSHERNRCKRVTREALRLMHPTLAQGYDVVVIVRGGVEELTGRDVAERS
                     LGEIFRKTGLLARER"
     gene            1509..1730
                     /gene="yidD"
                     /locus_tag="VHR64_15160"
     CDS             1509..1730
                     /gene="yidD"
                     /locus_tag="VHR64_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX3304225.1"
                     /translation="MRNPIRWLMLKLIRGYQLIISPHMPPTCRYVPSCSEYGYEAIER
                     YGALKGGWMATKRVARCNPFAKGGHDPVP"
     gene            1936..3075
                     /locus_tag="VHR64_15165"
     CDS             1936..3075
                     /locus_tag="VHR64_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX3304226.1"
                     /translation="MPFDGALPFPAAIPIWTQYVDFLKWVLDGIAQAVGNGGLAIVIF
                     TIIVRTLILPITVRSIKSMKAMQDIQPKIKELQKKYKGDRMKIQQETMALYQTYGVNP
                     VAGCLPALLQLPIFFGVYRAILALSKSDTGVWHESFLWLHSLKDPDPWHILPIMAGIF
                     QLMQTFMSRPSGQGKVTDPQQAMMNTMMNIMPITVVLFGWGFAAGAVVYWVTQSLYGI
                     IQQWFITGWGKLNDYIPNLPELPEHRRLGYHPPKDLEELANLPPKKKGFFGTWWEKQM
                     HAAQQIQEERKTATAGAGAGGAPGAPAQSTSSARRPAAPKSERPYSRNSPKGRMLADQ
                     AKRAEAEDAASALEADIVAEDVQSADISANGAAKPTPKRNRRAKK"
     gene            3166..4011
                     /gene="jag"
                     /locus_tag="VHR64_15170"
     CDS             3166..4011
                     /gene="jag"
                     /locus_tag="VHR64_15170"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX3304227.1"
                     /translation="MRSNERLTSVEIQAVTVEDAIRLALDQLQLGREDVDIEILSDAG
                     TEEDEEALVRVTAKGMASQPVSASSGGRSATAGARSEYRDRGGQRRSPGPRRVPGDRP
                     SSRGAPPARVEREIDPNRVDAEDETQVKEIVRELLGHMGVDADVMAIDNPSILPMTEE
                     DAPTIFIDVVGTDLGMLIGRRGEHLAQFQYLVNLICSKQMKDARRVVIDVEGYRTRRE
                     ESLIGLAERVARQVSRSHRPMLMEPMPANERRIIHLKLREHPEVYTESTGEGPQRRVE
                     VRPRD"
     gene            4133..4990
                     /locus_tag="VHR64_15175"
     CDS             4133..4990
                     /locus_tag="VHR64_15175"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX3304228.1"
                     /translation="MIGHICADILPDGTAVLGGTALYSALAAATFGWRTGILTRGRYG
                     FNLGGLEIPPLTIGSERIQVVVQDAEWPTCFVNEYAPSGRRTQQITRWAGPIDLRGLP
                     PSWHTARVIHLGPIAQEIDVRQASGLNPQFLGTTPQGWMRDWPRGTGGRVQPTHLRLP
                     ADLISQLDGIVVNDEEFGYSRDMVEAVGKHGYGVVTLGENGARVFTPKGQITEPAFHV
                     PVVDLTGAGDVFSAAFFYRIADPSNTPNQALRFAHAAAGLSLGGVGSSHIPTRPEIRK
                     LMQRVADSV"
     gene            4995..6410
                     /gene="mnmE"
                     /locus_tag="VHR64_15180"
     CDS             4995..6410
                     /gene="mnmE"
                     /locus_tag="VHR64_15180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX3304229.1"
                     /translation="MTIDFGTIAAVATAPGEAGIAVIRVSGPEVREIVARVVRLGRSG
                     PPLMLDSGNSHQLFYGRVVDPTTDHVLDEVMVAWMAAPRSFTTEDIVEISCHGGAVAS
                     QQVLRAVLAAGARPANPGEFTMRAFLNGRIALTEAEAVLNVVSAQTSEGLERALDDLR
                     GDLTRRLEPARAAVLAALAYLDASADFPDDEIPVSDVHADLSHAIEALEDVLAGAKSG
                     RLLSEGATVALVGRPNVGKSSLLNALLRSDRAIVTSIAGTTRDVVSERAVIDGIPVTL
                     LDTAGIAESADVIEQAGIERSRQAMARAAAIVHVVDGSVAPIGEDLAITRDLAARFET
                     GALGDVPLVLAVNKADLEPRCDQSAVIGELPVGVLAVPVSAKTGEGLTDLEAAIANVL
                     RGDLGGAVQPSLLNARQHAELDRALLHLRAAFDALEVGFPTDVLATDVRVAARALANV
                     TGEDIDEALLVEIFSRFCIGK"
     gene            6719..6958
                     /gene="rpmB"
                     /locus_tag="VHR64_15185"
     CDS             6719..6958
                     /gene="rpmB"
                     /locus_tag="VHR64_15185"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX3304230.1"
                     /translation="MSRRCAISGKTTTFGRNVSFSKKRTNRRFEANIHYKNLYVPELG
                     VTIRCKLSTHALRTIDKKGLAAYLKDEGKTYRDLL"
     gene            7102..7365
                     /locus_tag="VHR64_15190"
     CDS             7102..7365
                     /locus_tag="VHR64_15190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX3304231.1"
                     /translation="MPTYDFRCPSCATLFEERRSFARADDPAVCPSCGGDRAAKVISV
                     AEFYSPGSAARSLLDPGARQTATAAPHAAGCPCCSPKRSVTPA"
     gene            7362..7892
                     /locus_tag="VHR64_15195"
     CDS             7362..7892
                     /locus_tag="VHR64_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX3304232.1"
                     /translation="MSNPDVKLSPDLHRAIAAEKIGQVQIAIVTVSDSRTPETDTNMH
                     YLRPQIEGFGHKVAGYELIKDEPEQVEAALNRFVESDARLILFNGGTGIAPRDTTYDV
                     LARKIEKPMPGFGELFRMLSYEQVGAAAMLSRATAGVYRGKLVISTPGSNAAVRLAWE
                     KLIAPELQHLAWEVVR"
     gene            7889..8368
                     /locus_tag="VHR64_15200"
     CDS             7889..8368
                     /locus_tag="VHR64_15200"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX3304233.1"
                     /translation="MSGIACQSTNPHAHHGVPGPAWIDATLNQLRHDKNRITQPRMAV
                     LHWIAGRDAPFTAEEAVAEIDRTHGSGSRATVYRFLLWLREQGWLARVHRTDRDHALV
                     RQLPGHHQVVCVSCGDTLVIGGCDLTPLIGQSLTGTGFAVEGHQLEIYGRCRSCAEA"
     gene            8383..9369
                     /locus_tag="VHR64_15205"
     CDS             8383..9369
                     /locus_tag="VHR64_15205"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX3304234.1"
                     /translation="MTSGSDMTRCWRSLGLALILILAIGAVPVRHASAQDSGRLKVVA
                     TFSILGDVVQNVAGDNVDLTVIVGPDGDAHTFEPKPDQIASLANANLIFENGIGFETW
                     LDDMYSASGSSAKRIAVTDGLPLLEFNGQADQPATHTSDDGHDHGEHDPHVWQDVSNV
                     MAEVGVIRDALITADPAHADGYTANAAAYTTQLQELDAKIKQMVGTLLAEKRVLVTSH
                     DSLGYFAHAYGFTIAGTALGSLSTEGADPSAGDIAKLIDQIKATGVPAIFAENVENQD
                     LMNQIARDAGVTLAPPLYTDALSKSDGPAATYIALMTYNATTIVTALGGQAG"
     gene            9374..10141
                     /locus_tag="VHR64_15210"
     CDS             9374..10141
                     /locus_tag="VHR64_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3304235.1"
                     /translation="MITLPYRREPCHGQTVPGEAAVAVDDLSISMPGNEQLALDGVSL
                     TVPIGSRTALVGPNGAGKSTLLKAIVGILKPRSGSIRVYGMPVASCLHRVAYLPQRGE
                     IDWRFPMTVRELIQTGRYVHLGWLRRPGSADRALVDQEITRLRLEELADRQIGQLSGG
                     QQQRTLLARALVQGADLLLLDEPMNAVDAETRMILIGVLDDLRAEGKTAIIATHDIGR
                     LESDFDDVLFLADGHQVTAEVAFAHEHVHAMPAGHAR"
     gene            10313..>10865
                     /locus_tag="VHR64_15215"
     CDS             10313..>10865
                     /locus_tag="VHR64_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX3304236.1"
                     /translation="MIDWLTEPFRYQFMLNGLFAAVMVGITCATLGSYVVLRRMAFIG
                     DALAHTTLPGLVVAYLQGWSLFGGAVMAGVVTALGIGWLSKRGDVREDTAIGIVFTGM
                     FAFGILLISTAQSFRDLTHMLFGNILGVTDSELRLIGLITLIVVVMLGLFHKELELTS
                     VDPVYADVIGLRTGLVRYGLLVLL"
BASE COUNT         1873 a         3467 c         3657 g         1868 t
ORIGIN      
        1 tgatgggctc tgcgcgcggc cgttccgcac ggcgagcagg ctcagccggc ggcgcggcga
       61 tcgctgcggt ggttgcggtc gcgctcggcg ccggctgagc ctgctggtaa aggctgagca
      121 ggatatcggc caccggcaga agcgttgcga cctccgcttg cagcgtcgcc gagcgcgcga
      181 tttcctgcgc ggccgctgcg gcgtcgtccg gcgcgaggac gccaagcacg agggcgccgg
      241 tcgcctcggc ccgttggagc gactcgggct ccacatcggg ttccggcgcc ggaccgtccg
      301 tctcgtcagc agcctccgtc gcggtatcga cgggctgcgc tgtcagatcg tccgccgccg
      361 gtgctgtctc ggcggtttcg acgtctcgct cgtcggattc ggcgtcgttt tcgggaaggg
      421 tggcctgttc gcggtcggtc aacgctcgga ttctcccaat tgctcgatca gggactgacg
      481 caggctgagg agtccggacc gcaaactagt ctcgaccacg tgcggatcga cctcgagctg
      541 ggtggccgcg tcgggcacgg acagtcgctc ccgcgtagcc aaccgcaggg cgtcgcgctc
      601 gagcggagag agctgtcgaa tcccttctcg gaggagctcc cgtgtgatct cggcgcggcg
      661 agcgaccagt ggatcgtcgg cggcggggga gatctccacg ccgggcgcaa ccgcgattga
      721 tggcagcgaa ctcacgaacc caatccttcc cgtacggcgc aattgtaggg gagacggcga
      781 gatttcagta gaatttcgtt tgacgcgctg gcgcaatgtc cgtatacttt gcgcttggcc
      841 gttgcggagg atactgcctg caacggcggt tttctgttcg gaatagacgc gtcgttttcc
      901 ggcgcggagc gtggattacg atgccgaagc gaacgtatca gccaaagcgc attccgcgtc
      961 ttcgggaaca tgggttcctc tcgcggatgg ccacccggaa cggtcgggcg attttgaccg
     1021 cgcgccgccg gaagggccga cacaagttgt ctgtgtcgga cgagggcaag ttcacacaga
     1081 acaagtagcg cgcaccggcc gtcagcaatg gcggccggtt ttgttttcac cccgcccgga
     1141 acgaatcccc tatggaccga tcactgcggc tccacgaatc atatgatgtg aagcagacgc
     1201 gctcccgcgg aaaatcgggc gcgagcggtc cgctggtggc ccgtgtcctg ccgaatgcgc
     1261 tcgatccggc ccgcaaccgg tacacggtcg tggccggcaa gaaggtcggc aagtcgcatg
     1321 agcgaaaccg ctgcaagcgc gtcacccggg aggcgctgcg tctgatgcac ccgacgctgg
     1381 cgcagggata cgacgtggtc gtgatcgtcc ggggcggagt cgaagagctg accgggcgcg
     1441 acgtggcgga gcgatcgctc ggcgagatct tccgcaagac gggtctgttg gcccgagagc
     1501 gataggcaat gcgcaacccg atccggtggc taatgctgaa gctaatccgg ggctaccagt
     1561 tgatcatctc gccgcacatg ccgcccacct gccggtacgt gccgagttgt tccgagtacg
     1621 ggtacgaggc gatcgagcgc tatggggcgc tcaagggtgg ctggatggca acgaagcggg
     1681 tcgcccgttg caacccgttc gcaaaaggcg gccatgaccc cgtcccataa cgatcggtgg
     1741 tcaacggaga gagcgatccg ggacgatttg ccagggcaaa gggtggccgt gaagcccctc
     1801 gttccgtaga cttctgcagg cagatctccg gatcgccctt ctcaagattt gtttccgggc
     1861 gcggccgttt ccccggttcc ccgtggccgc gccaatctga aagcgtcccg ctctcggcgg
     1921 gggaggccta caccgttgcc gttcgatgga gctttgccct ttccagccgc catcccgatc
     1981 tggacccagt acgtcgactt cctgaaatgg gtgctcgacg gcatcgccca ggctgtcggc
     2041 aacggcggcc tggcgatcgt gatcttcacg atcatcgtcc gcaccttgat cctgcccatc
     2101 accgttcgat cgatcaaatc gatgaaggcg atgcaggaca tccagcccaa gatcaaggaa
     2161 cttcaaaaga agtacaaggg cgatcggatg aagatccagc aggagacgat ggcgctgtac
     2221 cagacgtacg gtgtgaatcc ggtcgccggc tgtctgcccg cgctcctgca gctcccgatc
     2281 ttctttggtg tctaccgcgc catcctggct ctttcgaaga gcgatacagg cgtctggcac
     2341 gaaagcttcc tctggctgca cagcctgaag gacccggacc cgtggcacat cctgccgatc
     2401 atggccggca tcttccagct catgcagaca ttcatgtccc gcccgtccgg gcagggcaag
     2461 gtgaccgacc cgcaacaggc catgatgaac acaatgatga acatcatgcc gatcacagtc
     2521 gtcctcttcg gatggggctt cgccgcgggc gcagtcgtct actgggtgac gcagagcctc
     2581 tacggcatca tccagcagtg gttcatcacc ggctggggca agctgaacga ctacatcccg
     2641 aacctgcccg agctgccgga gcatcgccgg ctcggctacc acccgccgaa ggacctcgag
     2701 gaactcgcca atctcccgcc caagaagaag ggcttcttcg gcacctggtg ggaaaagcag
     2761 atgcacgccg cgcaacagat tcaggaggaa cgcaagactg ctaccgccgg cgcgggagcc
     2821 ggtggagcgc cgggggcccc agcgcagtcg acatccagcg cgcgccgccc cgccgcgccg
     2881 aaatctgagc gtccctactc acgcaactcg cccaagggcc ggatgctggc cgatcaggca
     2941 aaacgggccg aagcggagga cgccgcaagc gccctcgagg ctgatatcgt ggccgaagac
     3001 gtgcagtcgg ccgacatctc ggcgaacggg gccgccaagc ccacaccgaa gcggaatcgg
     3061 cgcgcgaaga agtagcgcgc tcagccttcg tgccaaacgc ggtactatcc gggggagatc
     3121 gacgatgatc cgcgccccgc aatcgggaga taccagacga gcaacatgag aagtaacgag
     3181 cggctcacca gtgttgagat tcaggcagta accgtcgagg acgccatccg gctcgcgctc
     3241 gatcagcttc agctcggccg tgaggatgtc gacatcgaga ttctgtccga tgccggcact
     3301 gaagaggatg aagaggccct ggtgcgggta accgccaaag gcatggcatc tcagccggtc
     3361 tcggcaagtt cgggcggtcg ctcggcgacc gccggcgcgc ggtcggagta ccgggatcgg
     3421 ggcggccagc gccgctcgcc gggtccacgc cgggttccgg gtgatcggcc gtccagccgg
     3481 ggcgcaccgc cagcccgggt cgagcgcgag atcgatccca accgggtcga cgccgaggac
     3541 gagacgcagg tcaaggagat cgtgcgcgag cttctcgggc acatgggcgt cgatgccgac
     3601 gtgatggcga tcgacaaccc atcgatcctg ccgatgaccg aagaggacgc gccgacgatc
     3661 tttatcgatg tggtcggcac cgacctcggc atgctgatcg gcaggcgtgg cgagcacctc
     3721 gcccagttcc agtacctcgt caacctgatt tgctcgaagc agatgaagga tgcccgccgc
     3781 gtcgtgatcg atgtcgaggg gtatcgcacg cgccgtgagg agtcgctgat cggtttagcc
     3841 gagcgggtgg cccggcaggt atcgcgtagc caccggccga tgctgatgga gccgatgccg
     3901 gccaatgagc gccggatcat ccacctcaaa ctgcgtgagc acccggaggt ctacaccgag
     3961 agcaccggtg agggcccgca gcgccgcgtg gaggttcgcc cgcgtgactg atcgccccgc
     4021 gcgaccgtcc cccgacggtt cggaggcgaa acggacttca tccgccgcgt cccgatccag
     4081 acaaagggga tcggaacgcg acacccgcca gcaacgcccg cccgactatg tcgtgatcgg
     4141 gcatatctgt gccgacatcc tgccggatgg gacggcggtg cttggcggta cggcgctcta
     4201 ctcagcgctg gcagccgcga cattcggctg gcgtaccggc atcctgactc gcgggcgcta
     4261 cgggttcaat ctcggcgggc tcgagattcc tcctctgacg atcgggagcg agcgaatcca
     4321 ggtcgtcgtc caggacgccg agtggccgac ctgtttcgtc aacgaatatg cgccatcggg
     4381 ccgtcgtacc cagcagatca cgcgctgggc cggtccgatc gacctgcggg gactgccgcc
     4441 atcgtggcat accgcgcgtg tgatccatct cgggccgatc gcgcaggaga tcgatgtccg
     4501 ccaggcgagc gggctcaacc cgcagttcct tggcacgacg ccgcagggct ggatgcgcga
     4561 ttggccgcgc gggaccggcg gacgggtcca gccgacgcac ctccgcctgc cagcggatct
     4621 gatcagccag ctcgacggaa ttgtggtcaa cgacgaggag ttcggctact cgcgggacat
     4681 ggtcgaggcg gtcggcaagc acgggtacgg tgtagtcacg ctcggcgaga acggggcgcg
     4741 ggtgtttacg ccgaagggac agatcaccga gccggcgttt cacgtgccgg tggtggatct
     4801 gaccggcgcc ggcgatgtct tctcggcggc cttcttctac cggatcgccg atccatcgaa
     4861 cacgcccaac caggccctac gatttgccca tgcggccgcc gggttgtcgc tcggcggcgt
     4921 cggctcgagc cacattccga cccgcccgga aatccgcaaa ctgatgcagc gggtcgccga
     4981 cagcgtctga cgccatgacg atcgatttcg ggacgattgc cgccgtcgcg accgcgccgg
     5041 gcgaagcggg cattgccgtg atccgggtca gtgggccgga ggtgcgcgag atcgtcgcgc
     5101 gggtcgtgcg gctgggccga agcggaccgc ccctaatgct cgactccggc aactcccacc
     5161 agctcttcta cggtcgcgtg gtcgatccga cgacggacca tgtgctcgac gaggtgatgg
     5221 tcgcctggat ggccgcgccg cgctccttta cgaccgagga catcgtcgag atttcgtgtc
     5281 atggcggtgc ggtcgcatcg cagcaggtgt tgcgggcggt gctagcagcc ggggcgcggc
     5341 cggctaaccc gggcgagttc acgatgcgcg ccttcctgaa tggccgaatt gcgctaaccg
     5401 aagccgaggc ggtgctgaac gtggtctcgg cgcaaacctc cgaggggctg gagcgtgccc
     5461 tcgatgacct gcgtggggat ctgacccggc ggctggagcc ggcgcgggcg gcggtgctgg
     5521 cggcgctcgc ctatctcgac gcctcggccg atttcccgga cgacgagatc ccggtcagcg
     5581 atgtccatgc cgatctttcg cacgccattg aggcgctcga ggatgtgctg gccggcgcga
     5641 agagcggtcg attgttgagc gagggcgcga ccgtggcgct ggtcgggcgg ccaaatgtcg
     5701 gcaagagcag cctgctcaac gcgctgctgc gatcggaccg ggcgattgtg acgtcgatcg
     5761 ccgggacgac gcgggacgtg gtcagcgagc gcgccgtgat cgacgggatt ccggtcacgc
     5821 tgctggacac ggccggaatt gccgagagcg cggacgtgat cgagcaagcg ggcatcgagc
     5881 ggtcgcggca ggcgatggcg cgggcggcgg cgatcgtgca cgtcgtagac gggagcgtcg
     5941 cgccgatcgg cgaggatctg gcgataacgc gggatcttgc ggcgcgattc gaaaccggcg
     6001 cgctgggcga tgttccgctg gtgttggcgg tcaacaaggc cgatctggag ccgcgttgcg
     6061 atcagtcggc agtgattggt gagttgccag tcggagttct ggcggtgccc gtctcggcga
     6121 agaccggaga ggggctgacc gacctggagg cggcgatcgc gaacgtgctt cgtggcgatc
     6181 tgggcggtgc ggtccagcca tctctgctca acgctcgcca gcacgccgag ctcgaccgcg
     6241 ccctgcttca cctccgggcg gcgttcgacg cgctcgaggt cggcttcccg acggatgtac
     6301 tggccaccga cgtcagggtc gcggcccgcg ccctggccaa cgtcaccggc gaggacatcg
     6361 atgaggcgtt gctggtcgag atcttctcgc ggttttgtat cgggaagtga ggttatggct
     6421 tcctggggtt cggggttcgg ggttcgggga cgagcgtaat cgttggtccg cccgaggttg
     6481 aaaccccggg ctgagtacac gaagaccctt tcagggactg atagcggagg ggtgggacca
     6541 aggggcaggg aacctgcccg gcgtaacttc gcgcctttgc tcctccgccc cccaactgct
     6601 cgcctgctcg cctctcgact gctcgcctca tttatggcaa aataccccct tgtgcggtga
     6661 ttccaccgct tttttgtgcg tgccttcatt tcgattgacc gatcaaagga ccgtagccat
     6721 gtctcgacga tgcgcaatca gcggcaagac gaccaccttt ggtcggaacg tctccttctc
     6781 gaagaagcgg accaaccgcc gctttgaggc caacatccac tacaagaacc tctatgtgcc
     6841 ggagctcggc gtgacgatcc gctgcaagct cagcacacac gcgctccgca cgatcgacaa
     6901 gaagggtctc gccgcctacc tcaaggacga aggcaagacc tatcgcgacc tgctgtaacg
     6961 caagccgtca caacctacga acctgaacga cccggagcaa ttgctccggg tttgtccttt
     7021 tactgacaca tcatcgcatt gacgttggat atcgcgactg ggtatcatct gggcgagtga
     7081 ccgtctggag gtctttgccc gatgcctacc tacgattttc gctgtccgag ctgtgcgacg
     7141 ttgttcgagg agcgccggtc cttcgcccgc gccgatgatc cggctgtttg cccgagctgc
     7201 gggggtgatc gcgcggccaa ggtgatctcg gttgccgagt tctattcgcc cggatcggcc
     7261 gccagatcgt tgctcgatcc cggcgcacgc cagacggcaa ccgccgcgcc gcatgccgcc
     7321 ggctgcccgt gctgctcgcc caagcgatcg gtgacgccgg catgagcaac cccgacgtca
     7381 agctgagccc ggatttgcac cgcgcgattg ccgccgagaa gatcggccag gtccagatcg
     7441 cgatcgtgac ggtcagcgac agccgcacgc ccgaaaccga caccaacatg cactacctgc
     7501 ggccgcagat tgagggattc ggtcacaagg tcgccggata cgaactgatc aaggacgagc
     7561 cggagcaggt cgaggcggct ctgaatcgtt tcgtggagtc cgatgcgcgg ttgatcctgt
     7621 ttaacggcgg caccggaatc gccccccgcg acacgaccta tgacgtcctc gcccgcaaga
     7681 tcgagaagcc gatgccgggc ttcggcgagc tcttccggat gctcagctac gagcaggtcg
     7741 gcgcggcagc aatgctctcc cgggcgacgg ccggggtcta ccgcggcaag ctggtcatct
     7801 cgacgcccgg ctcgaatgcg gcggtccggc tggcctggga gaagttgatc gcacccgagc
     7861 tccaacacct ggcctgggag gtcgtgcggt gagcggcatt gcctgccagt cgaccaatcc
     7921 gcacgcgcat catggtgtgc cgggcccggc ctggatcgac gcgaccctga accagctccg
     7981 gcacgacaag aaccggatca cgcagccccg gatggccgtc ctgcactgga tcgccgggcg
     8041 ggatgcgccc ttcacggccg aggaggcggt ggcggagatc gatcggaccc acggctcggg
     8101 gagcagggcg acggtctacc gcttcctgct ctggctgcgc gagcagggct ggctggcgcg
     8161 ggtgcaccgg acagaccgcg accatgcgct ggtgcggcag ctgcccgggc atcatcaggt
     8221 cgtctgcgtc tcgtgcggcg acacgctggt gatcggtggg tgcgatctga ccccgctgat
     8281 cggccaatcg ctgaccggca ccggatttgc ggttgagggc catcaactcg agatctacgg
     8341 ccgctgtcga agctgcgccg aggcctagcg gcggctcatc gcatgacgtc tggatctgac
     8401 atgacacgct gctggcgctc tctcgggctc gcgctcatcc tgattctcgc aattggcgcc
     8461 gtaccggtgc ggcacgcgtc ggcgcaggat tccggccggc tgaaggtggt cgccacgttc
     8521 agcatcctcg gcgacgtggt ccagaacgtg gccggcgaca atgtcgacct gaccgtgatc
     8581 gtcgggccgg acggcgacgc gcacaccttc gagccgaagc cggaccagat cgcctcgctg
     8641 gccaacgcca atctaatctt cgagaacggg atcggcttcg agacgtggct ggacgacatg
     8701 tattcggcgt ccggatcctc ggcgaagcga atcgccgtga cggatggcct gccgctgctc
     8761 gaattcaatg gacaggcaga ccaaccggcc acgcacacga gcgatgacgg gcacgaccac
     8821 ggcgagcacg atccgcacgt ctggcaggac gtcagcaacg tcatggcgga ggtcggcgtg
     8881 atccgcgatg cgctgatcac ggccgatccc gcccatgcag atggctacac agccaacgcg
     8941 gcggcctaca cgacgcagct ccaggagctg gacgcgaaaa tcaagcagat ggtcgggacg
     9001 ctgcttgccg aaaagcgcgt cctcgtcacc tcgcatgact cgctcggcta ttttgctcac
     9061 gcgtatggct tcacgatcgc cggaaccgcg cttggctcac tgtcgacaga gggagcggat
     9121 ccatcggccg gagacatcgc gaagctgatc gaccagatca aggcgaccgg cgtgccggcg
     9181 atctttgccg agaacgtcga gaaccaggat ctgatgaacc agatcgcgcg ggatgccggc
     9241 gtgaccctcg cgccgccgct ctacaccgat gccctcagca agagcgacgg tccggccgca
     9301 acctacatcg ccctgatgac ctacaacgcg accacgatcg tgaccgcgct cggcggacag
     9361 gcgggatagg gcgatgatca cactccccta ccgccgcgag ccctgccatg ggcaaaccgt
     9421 ccccggcgag gcggccgtcg ccgtcgacga tctctcgatc tcgatgccgg gcaatgagca
     9481 actcgctctc gacggcgtca gcctgaccgt gccgatcggg tcgcggacgg cactggtcgg
     9541 accgaacggg gccggcaagt ccaccctgct caaggcgatc gtcggcatcc tcaaaccgcg
     9601 ctccggctcg atccgggtct atgggatgcc ggtcgcgagc tgcctgcacc gagtggcgta
     9661 tttgccgcag cggggcgaaa tcgactggcg cttccctatg accgtgcgcg agctgatcca
     9721 gaccggtcgt tatgtccacc tcggctggtt gcggcggcca gggtccgcgg atcgcgcgct
     9781 cgtcgatcag gagatcacgc ggctgcggct cgaggagctg gcggaccggc agatcggcca
     9841 actttcgggc ggccagcagc agcggaccct cctggcgcgg gcgctggtcc agggcgccga
     9901 tctgctcctg ctcgacgagc cgatgaacgc ggtcgatgcc gagacgcgga tgatcctgat
     9961 cggcgtgctc gacgatctgc gggccgaggg caagacggcg atcatcgcca cgcacgacat
    10021 cggccggctg gagtcggatt tcgacgacgt cctcttcctc gctgacggtc atcaggtgac
    10081 cgcggaagtg gcgtttgcgc atgagcatgt gcacgcgatg ccggccgggc acgcgaggtg
    10141 aggatgaaaa ccctgcgtag ctcggggtta ccacgccccg catggtgcat cctcacgctc
    10201 cgtgaatggt gcctgtgcgt agatggcact ggatccccaa ttggacggcc gaaatcggga
    10261 tcgggcaggg cacggcggcc ccgcgctacg tttccaaccc ggattcttca atatgattga
    10321 ttggttgacg gagccgtttc ggtatcagtt catgctcaat gggctgtttg cggcggtgat
    10381 ggtcgggatc acctgcgcga cgctcggctc atatgtcgtg ctgcggcgga tggccttcat
    10441 cggcgatgcg ctggcccata cgacgctgcc cgggttggtg gtcgcttacc tgcagggctg
    10501 gagtctgttc ggcggcgcgg tgatggccgg cgtggtgacc gcgctcggga tcggctggct
    10561 ctcgaaacgg ggcgatgtgc gggaggatac ggcgatcggg atcgtcttca ccggcatgtt
    10621 cgccttcggc atcctgctga tcagcaccgc gcagtcgttt cgcgacctga ctcatatgct
    10681 cttcggcaat atcctcggcg tgaccgacag cgagctgagg cttatcggcc tgatcacgct
    10741 gatcgtggtc gtgatgctcg gcctgtttca caaggaactg gagctgacat cggtcgatcc
    10801 agtctatgcc gacgtgatcg gcctgcggac cgggctggtc cgctacgggt tgctggtgct
    10861 gctgg
//
LOCUS       DATAOZ010000609         4158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8656652, whole genome shotgun sequence.
ACCESSION   DATAOZ010000609 DATAOZ010000000
VERSION     DATAOZ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8656652"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..920
                     /locus_tag="VHR64_15220"
     CDS             192..920
                     /locus_tag="VHR64_15220"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX3304237.1"
                     /translation="MTIADQTRPLTTPETVTTAQPATAFTVYWLFQADPNRRLATDPE
                     REQAAQSMLDAITRHGESVQVRGAYSTTGLSAGVDLILWLVADDCDAFQSLASEIHRS
                     PAGRALTLRHVYLGIASMSQYDPAHSPAFLRGLAPKKYLSVYPFTKTTDWYLLPYEQR
                     RDLMVEHGRMGQEYPSIQTNTVNSFGIADQEFIVALEDDDPATLVKMVQRLRAAEVRK
                     YTSIDTPIFLGRLTDTKTAIQNAL"
     gene            1059..2909
                     /gene="uvrC"
                     /locus_tag="VHR64_15225"
     CDS             1059..2909
                     /gene="uvrC"
                     /locus_tag="VHR64_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX3304238.1"
                     /translation="MSAKRRPASRPDFSSRLSALPTQPGVYIMKNAADEVLYVGKAQS
                     LRNRVRSYFQSQIRHDPKTRELVSQIADFEVIRTDTPTEALILENELIKRHWPRFNIR
                     LKDNSTYPYLKITNEEWPRVISTRKILKDGARYYGPYTSAGKAYETLNLLNRLFPYRK
                     CEQKITGHEQVCLYYHMKQCSAPCIAAVTNEGYMASIDAAALFLSGRGDEILAPLEEE
                     MQQASDGWNFERAAELRDRISAVRHVLERQKVVNVNGATADIVAVAQGPGGDAGVQVS
                     FIRNGKTLGSEFFEMQASIADAPASILSTFLTQFYGEAAMVPATIYIQHALPDEDRQV
                     VVDFLAERRGGKVELVVPQRGERRALVQMVAKSATDNLEQARIKHLSDEMKVTAALTE
                     LAEALELPRMPRRIECYDISNIQGTNPVASMVVFVDGRPARKEYRKFAIKTVEGANDF
                     AMMAEVIRRRFRRAKDMEGDEAGKWTDLPDLVIVDGGKGQLNAALGALEEVDMQVSIC
                     GLAKENEELFLPGRSESILLPRDSQALFLVQRIRDEAHRFALTFHRAKRSKATFQSSL
                     DSVPGVGPSRKKALIRKFGSVKAIREASITELAAVDGISTKLAEQIKASL"
     gene            3093..>4158
                     /gene="obgE"
                     /locus_tag="VHR64_15230"
     CDS             3093..>4158
                     /gene="obgE"
                     /locus_tag="VHR64_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX3304239.1"
                     /translation="MLVDQARIYVKAGDGGNGAMSFRREKFVPKGGPDGGDGGHGGDV
                     RLRVKQNLTSLLPFKFKAHFKAGSGQQGGGRNMHGKNGKTLHVDVPPGTIVYDDATGE
                     PLADLTDPGEEYVVAKGGKGGLGNQHFKSSTRQAPRIAELGEPGDDRWVRLELRIIAD
                     VGLVGLPNAGKSTLLAASSAAKPKIADYPFTTLQPNLGVVQLGGPGGQTYVMADVPGL
                     IEGAAGGSGLGHEFLRHITRTKALVHVLDASGGLEERDPIADFETINTELGDYDPELI
                     ERPMVVVLNKIDIPEAQANLPRLRSYLSERKYEVFEISAVTGEGVPALLNRVGAILRE
                     IAERPPKLVEPEIRTYSLADL"
BASE COUNT          802 a         1362 c         1281 g          713 t
ORIGIN      
        1 ccggatcgcg cggatcgagg cggcattgcc cgacggcgtc gcactgaccg gcagcccata
       61 ccggggcatc ggaattccgg acatcgtcca ccagtcgtgg cagctcgccg gccagctcgg
      121 gaagacagtg gctaaggcac cagttttggc cgaattgaac gcgtttcgtt gatagatacg
      181 gagacaccgt tatgacgatt gcagaccaga ctcgacccct gaccacgccc gagacggtga
      241 ccacggccca gccggcaacc gcgttcaccg tttactggct cttccaggcc gatccaaacc
      301 ggcgactcgc aacagaccca gagcgcgaac aagccgcgca gtcgatgctt gatgcgatca
      361 ctcgtcatgg cgagtcggtc caggtacgcg gcgcctactc gacaaccggg ctctccgccg
      421 gcgtcgacct gatcctctgg ttggttgccg acgactgcga cgccttccag agcctggcca
      481 gcgagattca ccgctcaccg gcaggtcgag cgctcaccct ccggcacgtt tacctcggca
      541 tcgcctcgat gtcgcagtat gaccccgccc actcgccggc cttcttgcgc ggtctggcgc
      601 cgaagaaata cctcagcgtc tatcccttca ccaagacgac cgactggtac ctgctgccgt
      661 acgagcagcg gcgcgatctg atggtcgagc acggccggat gggccaggaa tacccgtcga
      721 tccagaccaa cacggtcaac tccttcggga tcgccgatca ggagttcatc gtcgcgctcg
      781 aggacgacga tccggccacg ctggtcaaga tggtccagcg cctgcgcgcg gcagaggtcc
      841 gcaagtacac cagcatcgac accccgatct tcctcggccg cctgaccgac acgaagaccg
      901 cgattcagaa cgcgctgtag gcggttttcg gttttcggtt tttgggtttt cggacgaagg
      961 ctaaccgcag gggccggcat ctgcatggtc cctgcttcgc gtccatgcga ctctgcgtcc
     1021 tcctctcgac tcctcgactt ctcgactaaa atacgaacat gagtgcgaag cgacgccccg
     1081 cgtcccgtcc tgatttcagc tcccggctct ctgccctccc gactcagccc ggcgtctaca
     1141 tcatgaagaa cgccgcggat gaggtgctct acgtcgggaa ggcgcagtcg ctgcgcaatc
     1201 gcgtccgctc ctacttccag agccagatcc ggcacgaccc gaaaacgcgc gagctggtca
     1261 gccagatcgc cgatttcgag gtgatccgaa ccgacacgcc gaccgaagcg ctgatcctcg
     1321 agaacgagct gatcaagcgg cattggcccc ggttcaacat ccggctcaag gacaattcga
     1381 cctacccgta cctcaaaatc acgaacgagg agtggccccg ggtcatctca acccgcaaga
     1441 tcctcaagga cggggcgcgc tattacgggc cctacacctc ggccggcaag gcatacgaga
     1501 cgctcaacct gctcaaccgc ctcttcccct accgcaagtg cgagcagaag atcaccggcc
     1561 acgagcaggt ctgcctctac taccacatga agcagtgctc ggcgccgtgc atcgcggccg
     1621 tgacgaatga gggctacatg gcctcgatcg atgccgccgc tctcttcctg agtggccggg
     1681 gcgacgagat tcttgccccg ctcgaggagg agatgcagca ggcctcggac ggctggaact
     1741 tcgagcgcgc ggccgagttg cgggatcgga tctcggccgt ccggcacgtc ctcgaacggc
     1801 agaaggtggt caacgtcaac ggcgcgaccg ccgatatagt ggcggtcgca caaggtcccg
     1861 gcggcgatgc cggcgtgcag gtttcgttta tccgcaatgg caagacgctc ggctccgagt
     1921 tcttcgagat gcaggcctcg atcgccgacg cccccgcctc catcctctcg accttcctga
     1981 cccagttcta tggtgaggcg gcgatggtcc cggccacgat ctacatccag cacgcgctgc
     2041 cggatgagga tcggcaggtc gttgtcgact tcctggccga gcggcgcggc ggcaaggtcg
     2101 agctggtcgt accgcagcga ggcgagcgcc gggcactggt ccagatggtc gccaagagcg
     2161 cgaccgacaa cctcgagcaa gcgcggatca agcatctctc agacgagatg aaggtcaccg
     2221 ccgccctgac cgagctggcc gaggcgctgg agctgccgcg catgcctcgc cggatcgagt
     2281 gctatgacat ctcgaatatc cagggcacga atccggtcgc aagtatggtt gtcttcgtgg
     2341 acggccgacc ggctcgcaag gagtaccgca agtttgcgat caagacagtc gaaggcgcga
     2401 acgacttcgc gatgatggcc gaggtgatcc gtcgccgctt ccgccgcgcg aaggacatgg
     2461 agggcgacga ggccgggaag tggaccgatt tgcccgacct ggtgatcgtc gatggtggca
     2521 agggccaact caatgccgct ctcggcgcgc ttgaggaggt tgacatgcag gtctcgatct
     2581 gcggtctggc caaggagaac gaggagctct tcctccctgg gaggtccgag tcgatcctgc
     2641 tcccgcgcga ctcgcaggcg ctcttcctgg tccagcgcat ccgggacgag gcccaccgat
     2701 tcgccctgac cttccatcgg gccaagcgca gcaaagcgac gttccagtcc agcctcgact
     2761 ccgtcccggg cgtcggcccg agtcgcaaga aggcgctgat ccgcaaattc ggctcggtca
     2821 aggccatccg cgaagcttcg attaccgagc tggccgccgt cgacggcatc tcaacgaagc
     2881 tggccgagca gatcaaagcg tctctatagg cgagaagtcg agcaggcgag cagatgcggt
     2941 cagggttcgg gaatcaggta gccgaacatc caaaacctgg gacccaaaac ctaaaaccta
     3001 agccttcgcc tcgacgttca ctcgttgatc acggatactt ttctgtcgcg gggccgatgg
     3061 ttcggcctct tttcgtgttt taggcttgcc ccatgttggt cgatcaggcg cgtatttatg
     3121 tgaaagcagg cgatggcggg aacggggcaa tgtccttccg ccgcgagaag ttcgtgccca
     3181 agggcggtcc cgatggcggc gatggcggtc atggcggtga tgtccgcctc cgcgtcaagc
     3241 agaacctgac cagcctcctc ccgttcaaat tcaaggccca cttcaaggcc gggagcggtc
     3301 agcaaggcgg tggccgcaac atgcacggca agaacggcaa gacactccac gtcgatgtcc
     3361 cgccaggaac catcgtctac gacgacgcga ccggcgagcc cctggccgac ctgaccgacc
     3421 ccggcgagga atacgtcgtc gccaagggtg gcaagggcgg gctcggcaac cagcatttca
     3481 aaagctcgac ccgccaggcg ccgcgcattg ccgagctagg cgagccgggc gacgatcgct
     3541 gggttcgcct cgagctgcgg atcatcgccg acgtcggttt ggtcgggctg ccgaacgcgg
     3601 gcaagagcac gttgctcgcc gcatcttcgg ccgccaagcc aaagatcgcc gattacccgt
     3661 tcacgacact ccagccgaac cttggcgtag ttcagctcgg tggtccgggc ggccagacct
     3721 acgtgatggc cgatgtgccg ggtctgatcg agggggcggc cggcggatct ggactcgggc
     3781 atgagttcct gcgccacatc acccggacca aggcactcgt ccacgtgctg gatgcctcgg
     3841 gtggacttga ggaacgagac ccaatcgccg acttcgagac gatcaacacc gagctcgggg
     3901 actacgatcc ggaattgatc gagcggccaa tggtcgtcgt cctgaacaag atcgacatcc
     3961 cggaggcgca ggcaaacctt ccccgcctgc ggagctacct gagcgagcgt aaatacgagg
     4021 tgttcgagat ctcggccgtg actggtgaag gggttccggc ccttctcaat cgggtcggcg
     4081 cgatcctgcg tgagattgcc gagcgaccgc cgaagctggt cgagccggaa attcgcacgt
     4141 actcgctggc cgatctag
//
LOCUS       DATAOZ010000610         4722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1551009, whole genome shotgun sequence.
ACCESSION   DATAOZ010000610 DATAOZ010000000
VERSION     DATAOZ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551009"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2653
                     /locus_tag="VHR64_15235"
     CDS             <1..2653
                     /locus_tag="VHR64_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEX3304240.1"
                     /translation="DTSISRYVLGFDNGLGSGVAAVPWDWVPEAIDAERPTMPTWSLP
                     APPRSLEPLPEDAVKAVKQAVKTLNERLERIKQDYPPVGRLCLTGHAHIDLGWLWPVP
                     ETRRKTQRTFSTVLDLMDRYPDFTFNQSSAQAYAWMEEDDPGVFARIKERVAEGRWEP
                     SGGMWLESDCNVTGGEALVRQVVYGQRYFERTFGKRNRVAWLPDVFGFSGGMPQILRG
                     AGIDSFFTIKMYWNESNLFPYDLFQWEGIDGSRVLAHLFLNPGHGYNGNIVPLDTLGT
                     WRNFRAKTKHPESLLAFGWGDGAGGPTEKMLENYARIKDFPALPRLRMGSIEQFYASV
                     DREVLPSYVGELYFELHRGTLTSQARTKWLNRASEHRLVEAEAFAAIATLGGYPYPGE
                     ELETAWKTLLLNQFHDILPGSSINEVYKDTIPELEGVVATAIRIREEALAQISGVEGD
                     DTTAGHVLVANAGLTPRPLRGLLQGRSAASATVAADGTPLITQATPEGLLISDPDRMI
                     PGLGWTVVKMGGAESGRAAEGAGHVRAEASGGGAVLENDEIRVEIGADGTITRLFDKA
                     NNREAMADRANQLWAYVDKPRSWDAWDVDETYERDGEEVGGVEKIEILASGPVLGAVR
                     VARTFRESRIVQTYHLWAGFRRLEIETEIDWHERQVLLKARFPVAVRTHEATYETMYG
                     VVRRSTRRNTSWERAQFEGSAHRFIDMSEPGYGVAILNDGKYGHNARENVMSISLLRS
                     PLYPDPLADEGEHRFTYAVFPHAGDWTEAGVAAEAYALNSRLITVAAGPDTADGAGFV
                     RVEGTALGLGTLKQAFDGDGLVLRVYEPNGARGPVTLRFAEPVKAVERVNLLEEPEDG
                     AIEPGDGGRSASFDVRPFEVISLRIRR"
     gene            2658..>4722
                     /locus_tag="VHR64_15240"
     CDS             2658..>4722
                     /locus_tag="VHR64_15240"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX3304241.1"
                     /translation="MIIHMIGNAHIDPVWMWSWQHGADESLATIRSAADRCDEYPEFI
                     FTRGEVWVYQLVERIDPDLFGRIQPLIERGQWSVVGDQFLQPDCNLPTEKAWREQFAH
                     GRRYFSDRFGTRSTVAYNVDSFGHPATLPDILAGEGYTGYVIHRPNEAQVSLPAAAFR
                     WRGSGGGEVMGFRIAPAYVTRTDDLYGQLMLAIETADPTLGHTMCFYGVGNHGGGPTK
                     GNIEWIMANRDFEGHELRFSSPQIFFDTIAAQREQLPLVEVELQHVFPGCYSVMHDIK
                     QQQRHGELLLERAEQAANVFAEGAERDALLQRVDQAWPDLLFTEFHDILSGTSIPSAW
                     GGVRAMQGRAWITGEEVIYDASRRWARRTLPGAEFQQIAVVNPGPQDWSGIVEAEPWL
                     EFDIWDTRWLSTPDGQPVDFQQAQPEAQQGVNRVLFPADVPAGGAVQVLVRDDPAPDY
                     TPETDLDVSAIYLANSRIRVDLERGCIGDITVDGRSLLGKNGIRLHLRQDSSDTWGFL
                     SDRFDEPVSAIFDDAGWVIEEIGPLRARVRTEGWLGHSWIRWTLSLHRNDPRLQMQVE
                     VNFAERYSVLQMPIHLAAPPSRWTDGLAGGMVERTPNPAEWPVNGWSRIEVGETPLAM
                     VTHDAWSLSLADDVWQWTLLRSPRMSWGGRDPEIYAGRDWHTDQGPHSFAFALIPGMP
                     LDVPAL"
BASE COUNT          776 a         1417 c         1752 g          777 t
ORIGIN      
        1 cgacacctcg atttcccggt atgtgctggg cttcgacaat ggcctgggca gcggtgtggc
       61 ggcggtgccg tgggactggg tgccggaggc gatcgatgcc gagcgcccga cgatgccgac
      121 ctggagcctg ccggcgccgc cgcgctcgct cgagccattg ccagaggacg cggtcaaggc
      181 cgtcaagcag gcggtcaaga cgctgaacga gcggctggag cggatcaagc aggactatcc
      241 gccggttggt cgcctgtgcc tgacgggcca tgcccatatc gacctgggct ggctctggcc
      301 ggtgccggag acgcggcgga agacgcagcg gaccttctcg accgtgctcg acctgatgga
      361 ccgctacccc gatttcacct tcaaccagtc ctcggcgcag gcctatgcct ggatggagga
      421 ggacgatccg ggcgtctttg cgcggatcaa ggagcgggtg gccgaggggc ggtgggagcc
      481 gagcggcggg atgtggctcg agtccgactg caatgtgacc ggtggcgagg cgctggtgcg
      541 gcaggtggtc tacgggcagc gctacttcga gcggacattc ggcaagcgca accgggtggc
      601 gtggctgccg gatgtgttcg gcttctcggg cgggatgcca caaatcctgc gcggggcggg
      661 gatcgacagc tttttcacga tcaagatgta ctggaatgaa tcgaacctgt tcccatatga
      721 cctgttccag tgggagggga tcgacggcag ccgcgtcctg gcgcacctgt tcctcaaccc
      781 cgggcacggc tataacggca acatcgtgcc gctcgacacg ctcggcacct ggcgcaactt
      841 ccgggccaag acgaagcatc ccgagagctt gcttgccttc ggctggggcg atggggcggg
      901 cggaccgacc gagaagatgc tcgagaacta cgcgcggatc aaggatttcc cggcgttgcc
      961 gcgtctgcgg atgggcagca tcgagcagtt ttacgcgtcg gtcgatcgcg aggtattacc
     1021 gagctatgtc ggtgagctct acttcgagct gcatcgcggc accctgacca gccaggcccg
     1081 gaccaagtgg ctcaaccgcg cgagcgagca ccggttggtc gaggcggagg cgttcgccgc
     1141 gatcgccacg cttggcggct atccgtatcc gggtgaggag ctggagacgg cctggaaaac
     1201 gctgctgctc aaccagtttc acgacatcct gcccggctcc tcgatcaacg aggtctacaa
     1261 ggacacgatc ccggagctcg aaggggtcgt cgcgacggcg atccggatcc gggaggaggc
     1321 gctggcccag atctcgggcg tcgagggtga cgacacgacg gccgggcacg tgctggtcgc
     1381 gaatgccggg ttgacgccgc ggccgttgcg cgggttgctc caggggcgga gcgcagcgtc
     1441 ggccacggtc gcggccgatg ggactcccct gatcacgcag gccacgcctg aaggattgtt
     1501 gatctcggat ccggaccgga tgattccggg gttgggctgg accgtggtca agatgggcgg
     1561 cgcggagtcg ggccgggcgg cggagggcgc gggtcatgtc cgggccgagg cgtcgggcgg
     1621 cggtgcggtg ctcgagaatg acgagatccg ggtcgagatc ggcgccgacg gcacgatcac
     1681 gcggctcttc gacaaggcca acaaccgcga ggcaatggcc gaccgggcga accaactctg
     1741 ggcatacgtc gacaagccgc gctcgtggga tgcgtgggat gtcgacgaga cgtacgagcg
     1801 ggatggcgag gaggtcggcg gggtcgagaa gatcgagatc ctcgccagcg ggccggtgct
     1861 cggagcggtg cgggtcgcgc ggacattccg ggagtcgcgg attgtccaga cttaccacct
     1921 ttgggcggga ttccggcggc tcgagatcga gaccgagatc gactggcacg agcggcaggt
     1981 gctgctcaag gcccgcttcc cggtggcggt gcggacgcat gaggcgacct acgagacgat
     2041 gtacggcgtg gtgcggcggt ctacccggcg caacacatca tgggagcggg cccagttcga
     2101 gggctcggcg catcgcttca tcgacatgtc agagccgggc tacggcgtcg cgattctgaa
     2161 cgatggcaag tacggccata acgcgcgcga gaacgtgatg agcatcagcc tgctgcggag
     2221 cccgctctac ccggatccgc tggccgacga gggcgagcat cgcttcacct acgcggtctt
     2281 cccgcacgcc ggggactgga ccgaggcggg tgtcgcggcc gaggcgtatg cgctgaacag
     2341 ccggttgatc accgttgcgg ctggtccgga cacggcggat ggggcggggt tcgtgcgggt
     2401 cgagggcacg gcgcttgggc tgggcacgct caagcaggca ttcgacggcg acgggctggt
     2461 cctgcgggtc tacgagccga acggcgcgcg cggaccggtc acgctgcggt ttgccgagcc
     2521 ggtcaaggcc gtcgagcggg tcaatctgct cgaggagcct gaggacgggg caatcgagcc
     2581 tggcgatggc ggacggtcgg ccagcttcga cgtgcggccg ttcgaagtaa tctcgctccg
     2641 catccggagg tagcgccgtg atcatccaca tgatcggcaa tgcccacatc gacccggtct
     2701 ggatgtggag ctggcagcac ggggcagatg agtcgctggc gacgatccgc tctgccgccg
     2761 atcgctgcga cgagtacccg gagttcatct tcacgcgggg cgaggtctgg gtctatcagc
     2821 tggtcgagcg gatcgatccc gacctgttcg ggcggatcca gccgctgatc gagcgcgggc
     2881 agtggtcggt ggtcggcgat cagttcctcc agccggattg caacctgccg accgagaagg
     2941 cgtggcggga gcaattcgcg cacggccggc gctacttcag cgatcgattc ggcacgcgct
     3001 cgacggtggc gtacaacgtc gactcgttcg gccatcccgc gacgttgccg gacatcctgg
     3061 ccggtgaggg gtacacggga tacgtgatcc accggccgaa cgaggcgcaa gtctcgctgc
     3121 ccgctgcggc tttccggtgg cgaggatcgg gcggcggcga ggtgatgggg ttccggatcg
     3181 cgccggccta tgtcacccgg actgacgacc tctacggcca gctcatgctc gcgatcgaga
     3241 cggcggaccc gacgctgggc cacaccatgt gcttctacgg cgtcggtaat cacgggggcg
     3301 gtccaaccaa gggcaacatc gagtggatca tggccaaccg cgatttcgaa gggcacgagc
     3361 tgcgattcag ctcaccgcag atcttcttcg acacgatcgc ggcgcagcgc gagcagctgc
     3421 cgctggtcga ggtcgagctg cagcatgtct tccccggctg ctacagcgtg atgcacgaca
     3481 tcaagcaaca gcagcgtcat ggcgagttgc tgctggaacg ggccgagcaa gcggctaacg
     3541 tttttgccga gggtgcagag cgtgatgcgc tgctgcagcg ggtcgatcag gcgtggccgg
     3601 atcttctctt caccgagttc catgacatcc tctccgggac ctcgatcccg tcggcgtggg
     3661 gcggcgtacg ggcgatgcag gggcgggcgt ggattaccgg cgaagaggtg atctacgacg
     3721 cgagccggcg gtgggcgcgg cggacgctgc cgggcgccga attccagcag atcgcggtgg
     3781 tcaacccggg cccgcaggac tggagcggga ttgtcgaggc cgagccgtgg ctggagttcg
     3841 acatctggga cacgcgctgg ctctcgacgc cggacgggca accggtcgat ttccagcagg
     3901 cccagcccga ggcgcagcag ggcgtgaacc gggtgctctt cccggccgat gtgccggcag
     3961 gcggagcggt gcaggtgctg gtccgggatg acccggctcc ggattacacg ccggagaccg
     4021 atctcgatgt ctcggcgatc tatttggcga acagccggat ccgggtcgac ctggagcgtg
     4081 gatgcatcgg agatatcacc gtcgacgggc ggtctctgct cgggaagaac ggcatccggc
     4141 tgcatctgcg ccaggattcg tccgacacct ggggtttcct gagcgatcga ttcgacgaac
     4201 cggtctcggc catcttcgat gatgccggct gggtgatcga agagatcggc ccactgcggg
     4261 cgcgggtacg gacggagggc tggctgggcc actcctggat ccggtggacg ctgtcgctgc
     4321 accggaacga cccgcgcctg cagatgcagg tcgaggtcaa cttcgccgag cggtattcgg
     4381 tgctgcagat gccgattcac ctggccgcgc cgccgtcgcg ttggaccgat gggttggcgg
     4441 gcggaatggt cgagcggacg ccgaacccgg cggaatggcc ggtgaatggg tggtcgcgga
     4501 tcgaggttgg cgagacgccg ctggcgatgg tgacgcacga tgcctggagc ctgagcctgg
     4561 ctgatgacgt ctggcaatgg acattgctgc gcagtccgcg gatgtcgtgg ggcgggcgcg
     4621 atcccgagat ctatgccggg cgggattggc acaccgacca gggaccgcac agcttcgcgt
     4681 tcgcgctgat tccgggcatg ccactcgatg tgccggcact cg
//
LOCUS       DATAOZ010000611         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2346071, whole genome shotgun sequence.
ACCESSION   DATAOZ010000611 DATAOZ010000000
VERSION     DATAOZ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2346071"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /gene="rpiB"
                     /locus_tag="VHR64_15245"
     CDS             complement(<1..683)
                     /gene="rpiB"
                     /locus_tag="VHR64_15245"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX3304242.1"
                     /translation="MRIAIGSDHAGFALKEAVKAFLTAEHREVLDLGTHSTDPVDYSD
                     YAEAVGRALRENRAERGILLCGSGVGASMAANRIPGVRAGLCHDTYSAHQGVEHDDMN
                     VLVLGGRIVGVELARELIGAFLNARFTGEARHRRRLAKMIALENPLRALQVFGQSVWL
                     DYIRRSLITGGELSRLIDEDGLRGMTSNPAIFEKAITGSSDYRAVLEQFDILTLDAKS
                     IYERLAVQD"
     gene            complement(687..2804)
                     /gene="tkt"
                     /locus_tag="VHR64_15250"
     CDS             complement(687..2804)
                     /gene="tkt"
                     /locus_tag="VHR64_15250"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX3304243.1"
                     /translation="MSLTDAAAHAASDPIKPMSEPMDQLCVNTIRTLAMDAVQAANSG
                     HPGTPMALAPVVYCLWQRVLRFDPSHPIWPNRDRFVLSVGHASMLLYAMLHLSGVKAV
                     NPKYETLGQLSVTLDDIKLFRQLDSKCPGHPEYRWTSGVETTTGPLGQGLATSVGMAI
                     AERWLASYFNRPGFKLFNYDIYALCGDGCMMEGISGEAASLAGHLKLDNLCWIYDNNH
                     ITIEGNTALAFSDDVATRFIGHGWNVTRVGDANDLEMLERAFTTFRSTSDRPTLIIVD
                     SHIAYGAPNKQDTSAAHGEPLGEEEIRLAKRHYGWPPDAKFLVPDEVLAHFQDGIGAR
                     GLALHRAWWAQFEEYRRQYPELADEGYRMLRRELPDSWDKGFPAFSSGGKAVATRDAS
                     GQALNALAINVPWLLGGSADLGPSCKTRLTFEGAGDFSAENPAGRNLHFGIREHAMGA
                     ILNGLSLSKIRPFGSGFLIFSDYGRAAIRLSALMEIPVIHIFTHDSIGVGEDGPTHQP
                     VEQLASLRAIPGLITLRPADANEAVEAWRVIAGFRHEPVALILTRQAVPTLDRTKYAM
                     ADGVRRGAYVLADADDGRPHVLLLATGSEVALCVEAYETLKAEGIKARVVSMPSWEMF
                     EHYCRDHPEYREQVLPESVTARVSVEKGSTLGWARYVGTSGHSIGMETFGASAPLKEL
                     QKKFGFTPENIVALAKHQIAKAR"
     gene            complement(3010..>3582)
                     /locus_tag="VHR64_15255"
     CDS             complement(3010..>3582)
                     /locus_tag="VHR64_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304244.1"
                     /translation="AADWTYDVVSIGYPGPVLGGRPSAEPVNLGRGWIGFDFAQAFGR
                     PVKVINDAAMQALGSYQGGKMLFLGLGTGLGSALIANGMVEPMELGHLPYKKGTYEDY
                     VGRAGLERGGKKKWRRHVADVVERLMAALRPDDTVIGGGNVKKLDALPWRCRAGENAN
                     AFRGGFRLWVGTDVLPSNLPLGRARSKQKP"
BASE COUNT          654 a         1178 c         1065 g          685 t
ORIGIN      
        1 atatcctgaa ccgcgagtcg ctcgtaaatt gactttgcat ccaacgtcag gatgtcgaac
       61 tgctcgagaa ccgcgcggta gtcggagctt ccggtgatgg ccttctcgaa gattgccgga
      121 ttcgaagtca tcccccgcaa accgtcctcg tcgatcagac ggctcaactc accgccggtg
      181 atcaagctgc ggcggatata atccagccac accgattggc cgaagacctg gagcgcgcgc
      241 agcgggtttt ccagcgcgat catcttggcg aggcggcgcc gatgccgcgc ctcgccggtg
      301 aagcgagcgt tcaggaacgc gccaatcagc tcgcgggcga gttcgacacc gacgatacga
      361 ccgccgagca ccagcacgtt catatcatcg tgttcaacgc cctggtgtgc cgaataggtg
      421 tcgtggcaca gcccggcgcg gaccccggga atcctgttgg cggccatcga cgcgccaacg
      481 ccgctcccgc agagcaggat gccgcgctcg gcccggttct cgcgcaaggc tcgacccacg
      541 gcctccgcat aatcggaata gtccacggga tcggtgctgt gagtacccag gtcgaggacc
      601 tcgcgatgct ccgcggtcaa gaaggctttc accgcctcct taagtgcaaa tccggcatga
      661 tccgaaccta tcgcgatgcg catcgctcat ctcgctttcg caatctggtg cttcgccaat
      721 gcgacgatgt tctcgggagt gaagccgaac ttcttctgca gttccttcag cggcgccgag
      781 gcgccgaacg tctccatgcc gatgctgtga ccgctcgtgc cgacataccg cgcccagccg
      841 agggttgatc ctttttccac cgagacgcgt gcggtgaccg actccggcag cacctgctcg
      901 cgatattcgg gatgatcgcg gcagtagtgc tcgaacatct cccatgatgg catgctcacc
      961 acacgggcct tgatcccttc ggccttcagc gtctcgtacg cctctacaca cagcgcaact
     1021 tcactgccgg tcgccagcag caacacgtga ggccggccgt cgtcggcatc ggcgagaaca
     1081 taggcgccgc gccgcacgcc atccgccatc gcgtatttcg ttcggtccag cgtcggcact
     1141 gcctgccgcg ttaggatcag cgcgactggt tcgtggcgga agcccgcaat gacacgccag
     1201 gcctcgaccg cttcattggc atcggccggt cgcagggtga tcagcccggg gatggcgcgc
     1261 agggacgcca attgctccac cggttggtgt gttggcccat cttccccgac tccgattgaa
     1321 tcgtgggtaa agatgtggat caccgggatt tccatcaggg cgctcaagcg gatggccgca
     1381 cgtccgtagt cgctgaagat caggaacccg gatccaaacg gtcggatctt cgacagggac
     1441 aggccattga ggatagcgcc catcgcgtgc tcgcgaatcc cgaagtggag attccgcccg
     1501 gccggattct ccgcactgaa atctcctgcg ccttcaaaag tcagccgcgt tttgcatgac
     1561 ggcccgaggt ccgcggaacc gccgagcagc caagggacgt ttatggcaag tgcgttcagc
     1621 gcctggcccg acgcgtctcg ggtggcgacg gctttgccgc ctgaggaaaa cgccggaaat
     1681 cccttgtccc aactgtccgg cagctcgcgg cgcagcatgc gatacccctc atcggcgagc
     1741 tcgggatatt ggcgtcgata ctcctcaaac tgagcccacc aggctcggtg cagcgccaag
     1801 ccgcgcgccc cgatgccgtc ctggaaatgc gcgagtacct cgtcagggac caggaacttc
     1861 gcatccggcg gccagccgta atgacgcttc gccagcctga tctcctcctc gcccagcggt
     1921 tcgccgtgcg ccgcgctcgt atcctgcttg ttcggcgcac cgtaggcgat gtggctgtcc
     1981 acgatgatga gggttggtcg atcgctcgtg ctcctgaacg tcgtgaatgc acgctcgagc
     2041 atctctaggt cgttggcgtc gccgacgcga gtgacgttcc agccgtgacc gatgaagcgt
     2101 gtggccacgt catcgctgaa cgccagcgcc gtattgccct cgatggtgat gtggttgttg
     2161 tcgtagatcc aacagaggtt atcgagtttg aggtgtcctg ccagcgaggc ggcctcgccc
     2221 gagatccctt ccatcatgca gccgtcgccg cacagggcgt agatgtcata gttgaatagt
     2281 ttgaaccccg ggcgattgaa gtagctggcc agccagcgct cggcgatggc catgccgacg
     2341 cttgtcgcca gtccctgtcc cagtgggccg gtcgtcgttt cgacgcccga ggtccaacgg
     2401 tattccggat ggccggggca tttgctgtca agttgccgga aaagcttgat gtcgtccagc
     2461 gtcaccgaca attgtccgag cgtttcatat ttcggattga cggccttcac tccgctcaag
     2521 tgcagcatcg cgtaaagcaa catcgacgca tggccgaccg acaacacaaa gcggtcgcgg
     2581 ttcggccaga tcggatggct gggatcgaag cgcagcacgc gttgccacag acaatagacc
     2641 accggcgcca gcgccatcgg tgtgccgggg tgaccggagt tggcggcctg taccgcatcc
     2701 atcgccagcg tgcggatggt gttgacgcac aactggtcca tcggctcgct catcggcttg
     2761 ataggatcgg atgctgcgtg tgcagccgca tcagttaggc tcatcgttcc ttacctcccg
     2821 cacatgccga cggcgctgag gagaggacac ccgccggtgt gctctgaacg actttaggtc
     2881 aaaccggccc gcagcaactc agtaccacct gataagcgcc gccgagtcgg tcgcgtgctc
     2941 gcgcgcgcgg agattgccag tgacggttcg cgcgtcatca atcccgtcgg cgagctcatg
     3001 ttccgagcct cacggttttt gtttggagcg agcccgtccg agcggcagat tggacggcag
     3061 aacatcggtt cccacccaca agcgaaatcc gccgcgaaaa gcgttggcat tttcgcccgc
     3121 acgacaccgc caaggtagtg cgtcgagctt cttgacgttg cctcctccga tcaccgtgtc
     3181 atccggccgc agggcggcca tgagccgctc gacgacgtcg gccacatgac gccgccactt
     3241 cttcttaccg ccgcgctcca ggccggcacg tccgacgtaa tcctcgtagg tgcctttctt
     3301 gtacggcaag tgtccgagct ccatcggctc taccatgccg ttggcgatca aggcgctgcc
     3361 gagtcccgtt cccagaccca gaaacagcat cttaccacct tggtagctcc ccaaggcctg
     3421 catggcggcg tcgtttatca ccttgaccgg acgaccgaac gcctgcgcga aatcaaaccc
     3481 gatccagcca cgccccaggt tcacgggctc cgcagacggt cggccgccca ataccgggcc
     3541 tgggtatccg atggagacca catcataggt ccaatccgcg gc
//
LOCUS       DATAOZ010000612         8686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7869615, whole genome shotgun sequence.
ACCESSION   DATAOZ010000612 DATAOZ010000000
VERSION     DATAOZ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8686
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7869615"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VHR64_15260"
     CDS             complement(<1..944)
                     /locus_tag="VHR64_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX3304245.1"
                     /translation="MTADQFILAIDQGTTSSRALLFDQTGMAVALAQRPIDVTYPHPG
                     WVNQDANQIWTVTEAVCRDAMASADVDWSRIAAIGITNQRETTILWDRKTGEPVAPAI
                     VWQSRQSADVIRAMERRGVADRYREITGLVLDPYFSASKIRWLLDQDRDLQERAQRGE
                     IYFGTVDSWLIWKLTGGEHLTDITNASRTLLFDIRSCAWSDELLADLGIPSSILPAMV
                     PNSGEIASTSAEFGSILITGCAGDQHAALFGQACFAAGQAKNTYGTGSFALLNTGSDV
                     VVSNNGLLTTPAWAYRDQATYALEGSVLVSGSAVQWLR"
     gene            1008..2657
                     /gene="aroB"
                     /locus_tag="VHR64_15265"
     CDS             1008..2657
                     /gene="aroB"
                     /locus_tag="VHR64_15265"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX3304246.1"
                     /translation="MDIQRIVLIGFSGTGKSSIGRQLALRLGWTAIDTDRAIEIAAFK
                     TIPEIFASDGETAFRDIERQVLLRSLDCATAVIATGGGAVIGEEVWTSDTLLASGTLT
                     IALDSSPEVILNRLRSQQAMSNETIERPLLATTDPLRRITDLKRTRQPTYDRADLTLV
                     TDSMSESDVVNQIASLVIKSSEPDVVLDAPGGRSKIYVRPGIVEQTGALVSAQWPRAR
                     QAFVITDANVGPLHGDRVRVSLELAGLAVQTITVPAGEGSKGWESAGSVIGTMLEGGI
                     QRNDVVVALGGGVIGDLAGFAAAVVLRGVGIVQIPTSLLAMVDSSVGGKTGVNHSTGK
                     NLIGAFYQPPLVLADPEILRTLPERELTQGWAEVVKHAVIQPSTPDQFRSDIFGFLQR
                     NRDHLVGLREPALTYAIRRNIALKASVVEADEREANLRAILNYGHTIGHAIEAAEYRY
                     LHGEAISVGIHAANRIAALEGRIDVDREREIGNLLSAFGLPGRASFDPDVVRERMKSD
                     KKSTAGRQTWVLPTGPNGVELTTGVGNESIDSALDFVRSSE"
     gene            2834..4603
                     /locus_tag="VHR64_15270"
     CDS             2834..4603
                     /locus_tag="VHR64_15270"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3304247.1"
                     /translation="MSDTRISDLVGRLWSGELSRRDFIKRATAAGMGTSVISAALSQG
                     AMAAPGTRSSGSWSRYQADASTLIIADSLIGNQWLTLDPAWFYEINSTAAMNLVYEPL
                     YHIPDGTKPTEIVPLLATDFPSFSEDSLVATIKLRQGVKFHTSGNEMTSADVIFSWAR
                     LKNIGFQGSFFATDYWTDVKAVDDYTIQITLPAPNAALAAILTSMPLAITDSKRVQEM
                     GGTDATPAAQPTPAQGQEAPKSPEVQANEDARDQINGDTVGTGPFKGTGWDKSSEVTL
                     EAHADYWGEAPKLSQVIWSNTAEANAQVQRVQIGEVDIAYSLPVDQVDSVKSDSNLQV
                     LTANSLALCYMALNMVEAKGGPLAKKEVRQAIGYALDYQGYVGGIMGGAAVEPATAVP
                     LPLTGSEAVKDKGYTLDLAKAQQLWDSAGVGEQEITVSYDTDTVAPGGASYETIAVKV
                     KSDLEQIKGLKIKLAPAPGDERLAAYRAGDFQATLSPWTPDYPDVDSYCGPFARSNTA
                     AAKRVGYSDPEVDKLLDQGLSELDPTKREAIYVQIQEKMIDAAAFHVLYQPVDQKAAR
                     AVVQGAACHPVYQLQLRFASKTG"
     gene            4698..5720
                     /locus_tag="VHR64_15275"
     CDS             4698..5720
                     /locus_tag="VHR64_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3304248.1"
                     /translation="MGLWQFIARRIIQSVVVLFFVSVATFLLMHAVPGDPLVAVIGER
                     QADRPEIRAALEKRYSLDKPLPVQYISYVRNIIVNGDMGSTITTRRPVADELAKFIPF
                     TIELSIAAMIFALSVGVPLGIISALRQNRWQDHIARTLSLIGTAMPVFWLGLLLSYVI
                     SYKLRWLPRSGFKETGMKIPEHHTGFMTIDSIIAGDWAFLHSYLLHMILPAIVLGSFA
                     MGIVARMLRSSLVSALEDDYVRTARAKGLGEFSVVRGHALRNALIPTITVIGLTFASL
                     LAGAVLTETIFSWPGVGQFAVQMALKLDYPGLLGVTLFVAVAYIFVNLIVDILYGFLD
                     PRIRTG"
     gene            5731..6642
                     /locus_tag="VHR64_15280"
     CDS             5731..6642
                     /locus_tag="VHR64_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3304249.1"
                     /translation="MSTTSAIPITGVADPLSTVKRRTPAGDAIRTLRQRPVALFGLGV
                     LVAWILLGLLAPVLPLEAQNGQIKIENKLESPSREHPFGTDDAGRDVMARVIYAARTS
                     VPAGFLVVFCACAIGAMVGAASGYIGGFIDTVIMRACDGVLAFPSIILAMAIAVIRGE
                     PSLQNAMFAVVLVLWPEYARLMRGQVLSIRHNDYVMAAESLGATRRRVLFRHIMPATD
                     APIVVKATLDVGAAIVLTAGLSFIGLGAVPPTAEWGSMVSQASKTGVQYWWYHTFPGL
                     AIVSVVMALNFFGDGLRDALDPKRRAR"
     gene            6645..7136
                     /locus_tag="VHR64_15285"
     CDS             6645..7136
                     /locus_tag="VHR64_15285"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX3304250.1"
                     /translation="MIDPNLTGRPRVEAALEILGIEAAIVSPGTPMPTVPLAAEAVGC
                     TVDQIIKTVVFVTKDRHPVVAVANGTNRIDRKRLAAAIGVSTLKLADPEFVLDVTGYP
                     AGGVSPVGIRTLDAPIIIDGAVLEQDSVYGGAGTEDDLLHVATRDLVAVTGGRVCAIT
                     RGD"
     gene            7216..8325
                     /gene="ychF"
                     /locus_tag="VHR64_15290"
     CDS             7216..8325
                     /gene="ychF"
                     /locus_tag="VHR64_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX3304251.1"
                     /translation="MTTKLVILGLPSSGKTTVFNALTRSDAPTGGFSSATDEPNLATV
                     KVPDPRLDLLTEMFKPQRKVQADVQYLDVAGVAKGMAEKGMSGQLLGHLAQADALVHV
                     VRAFDDPNLPHPEGSVDPARDIETINLELAFSDLAIIEKRLQRIAAQLPKMRGAEREA
                     YEREQAAMETLKQALENETPLRDVVDALDPEDYKLLRGFGLLTAKPLLVLVNIGEDQI
                     GEAEQIEQRLSEQFSRSGVQVAALAGKIESEIAALDDDDAATFMEDLGITESSRDRVI
                     RLSFSLLGLMPFFTVGPDECRAWTVHRGDSAVEAAGAIHTDIQRGFIRAEVVSYDDLI
                     SAGSMAETKKHGTFRREGKTYVVQDGDIINFLFNV"
     gene            8333..>8686
                     /locus_tag="VHR64_15295"
     CDS             8333..>8686
                     /locus_tag="VHR64_15295"
                     /inference="COORDINATES: protein motif:HMM:NF025460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4272 domain-containing protein"
                     /protein_id="HEX3304252.1"
                     /translation="MSDYEDDALDIYLREPDDIGARIVLLTALSIWPELGASEEQRTW
                     RRWLDAQGVLQMATRDEEEALAGPVQSERIADLCERAVDALPALAWATALIDEIPLIE
                     PEDERAELIERIPVPD"
BASE COUNT         1647 a         2768 c         2668 g         1603 t
ORIGIN      
        1 tcgcgcagcc actggaccgc cgagcccgac accagcaccg agccttcgag ggcgtaggtc
       61 gcctgatcgc gatatgccca tgccggagtc gtcagtagtc cgttgttgct cacgacaacg
      121 tcgctcccgg tatttaagag agcgaatgag cccgtaccat aggtgttctt cgcctggccc
      181 gcagcaaagc aggcctgtcc aaagagcgcg gcatgctggt cgccggcaca tccggtaatg
      241 agtatcgacc cgaactccgc gctggtcgaa gcgatttcgc cggagttcgg caccatggcc
      301 ggcagaatcg atgacggaat accgagatcg gccaggagct cgtctgacca ggcgcacgac
      361 cggatatcga acaacagagt ccgcgaggcg ttcgtgatgt ccgtcaagtg ctcgccgccg
      421 gtcagcttcc agatcagcca ggagtcgaca gtcccgaaat agatctcgcc acgttgcgct
      481 cgctcttgca ggtctcgatc ctggtcgagc agccagcgga tcttgctggc cgagaaatat
      541 ggatcgagga cgagtcccgt gatctcgcga taccggtcgg cgacaccacg ccgctccatc
      601 gcgcggatca cgtccgcgct ctgccggctc tgccagacga ttgcgggcgc gaccggctcg
      661 ccggtcttac gatcccagag aatcgtcgtc tcgcgctgat tggtgatgcc gatggcggca
      721 atccgcgacc aatcgacgtc cgcacttgcc atcgcgtccc ggcaaactgc ctctgtcacc
      781 gtccagatct ggttcgcgtc ctggttgacc cagcccggat gcggataggt cacatcgatc
      841 ggtcgttggg caagcgccac agccatgccg gtctgatcga acagcaatgc tcgcgaggac
      901 gtcgtgccct ggtcgatcgc aaggatgaat tgatccgccg tcattcccat gcctccggtt
      961 agtatcctcc cataggatta tttggcgcgc agcggcggaa ctgggcagtg gatattcaac
     1021 gcatcgtcct gattggattc agcggcaccg gcaaaagctc gatcggccgg caattggcct
     1081 tgcgactcgg ctggacggcc atcgataccg accgtgccat tgagatcgcc gcgttcaaga
     1141 cgatccccga gatctttgcg tcggatggag aaacggcctt ccgggatatc gaacgccagg
     1201 ttcttcttcg ctctttggat tgcgcaactg ccgtaattgc gaccggcgga ggcgcggtca
     1261 tcggcgaaga agtgtggacc agcgacacgc ttcttgcttc cggaactctg accatcgccc
     1321 ttgattcatc gcccgaggtc atcctgaatc gcctcagaag tcagcaagcc atgagcaacg
     1381 agacgatcga gcgaccactc ctggccacga ccgacccgct gcggcgcatt actgacctca
     1441 agcgaaccag gcagcctacc tacgatcgcg ccgatctgac gcttgtgact gactccatgt
     1501 ccgaatccga cgtggtcaat caaatcgcct cactggtcat taagtcatct gagccggacg
     1561 tcgtgttgga tgcgccaggc ggtcgatcca aaatctacgt gcggcccggg attgtcgaac
     1621 aaaccggcgc gcttgtcagt gcacagtggc ccagggcgag gcaggcattc gtcataactg
     1681 atgcaaacgt cgggccgttg catggcgatc gcgtgcgcgt ctcactcgaa ctggccggcc
     1741 ttgctgtcca gacgatcacg gttcccgccg gggaaggcag caaagggtgg gagtccgccg
     1801 gatctgtcat cgggacgatg ctcgagggcg gaattcagcg caacgacgtg gtggtcgcgc
     1861 tgggcggcgg ggttatcggc gatcttgccg gctttgcggc cgcagtcgtc ctgcgcggcg
     1921 tcggcatcgt gcagatcccg acgagcctgc tggcaatggt cgatagctcg gtcggcggca
     1981 agaccggagt caatcattcc acgggcaaga acctgatcgg cgccttctat cagccgccgc
     2041 tggtccttgc cgatcccgaa attctgcgaa cgctgccgga gcgcgagctc acgcagggtt
     2101 gggctgaggt cgtcaagcac gcggtcatcc agccctcgac tccggaccaa ttccggtcgg
     2161 atatcttcgg cttcctgcag cgcaatcgcg accatctcgt tggtttgcgg gaaccggcgc
     2221 tgacgtacgc gattcgccgc aacattgcgc tcaaggcgtc ggtcgtcgaa gccgacgagc
     2281 gcgaggccaa cctccgcgcg atcctcaact atggccacac gatcggccat gcgatcgagg
     2341 cggccgaata ccgctatctg cacggcgagg caatctcagt gggtatccac gcggccaatc
     2401 gcatcgccgc gctcgaaggt cgaatcgatg tggaccggga gcgcgagatc ggcaatcttc
     2461 tctcggcttt cggccttccg ggcagggcgt cattcgatcc agacgtcgtc cgggagcgta
     2521 tgaaaagcga caagaagagc acagccggcc gccaaacatg ggtgctcccg accgggccga
     2581 acggagtcga gctcacgaca ggagtcggca acgaatcgat cgattccgcg ctcgactttg
     2641 ttcgtagctc tgagtaacgg actcccacgc ataccccctt ggcggaaggc cgcgcgtcgt
     2701 gaatacttcg ggcagactca ggaaccgaac acctgctcgt gcccttcgcg gcagcgccgg
     2761 tttcggcatc cctgcaggtt atgtcgtgtc gagtcgcact gccgcgctcg ccaattgacc
     2821 gtgaggagaa gagatgtcag acacgcgcat ttcggatctt gtcggtaggc tctggtccgg
     2881 ggaactctcc cgccgcgact tcatcaagcg ggcgaccgct gccgggatgg gcacctcggt
     2941 tatctctgcc gccctcagcc agggagcaat ggcggcgccc ggaacgagat cgagtggatc
     3001 ctggagccgg taccaggccg atgcctcgac actgatcatc gccgactccc tgatcggcaa
     3061 tcagtggcta acgctcgacc cggcctggtt ttacgagatc aactcgaccg ctgccatgaa
     3121 cctcgtctac gagccgctct atcacattcc cgacggaacg aagccgactg agatcgttcc
     3181 gctcctggcc acggacttcc cgtccttctc ggaggacagc ctcgtcgcga cgatcaagct
     3241 gcggcagggc gtcaagttcc acacgtccgg caacgagatg acgtcggccg acgtcatctt
     3301 ctcctgggcc cggctcaaga atatcggctt ccagggttcg ttcttcgcca ccgattactg
     3361 gacagatgtc aaggcggtcg atgactacac gattcagatc acattgccgg cgcccaatgc
     3421 cgcgctggcc gcgatcctga cctccatgcc actcgccatc accgacagca agcgggtcca
     3481 agagatgggt ggaacggacg caactccggc cgctcagccg acgcccgctc agggccagga
     3541 ggcaccgaag tcgccggagg ttcaggccaa cgaggacgcc cgcgaccaaa tcaacggcga
     3601 caccgtcggc accgggccgt tcaagggaac cggttgggac aagagcagcg aagtcacgct
     3661 cgaagcccac gccgattact ggggcgaggc gccgaagctc agccaggtca tctggtccaa
     3721 cacggccgag gcgaacgccc aggtccagcg cgttcagatt ggcgaagtcg atatcgccta
     3781 ttccctgccg gtcgatcagg tcgactccgt caagagcgat tccaatctcc aggtcctgac
     3841 cgccaatagc cttgcccttt gctacatggc gctaaacatg gtcgaagcca agggcggacc
     3901 gctggcgaag aaggaagtcc ggcaggcgat cgggtatgcg ctcgattacc agggctacgt
     3961 cggcggcatc atgggcggcg ccgcggtcga gccggccacg gcagtgccgc tgcccctgac
     4021 cggcagcgag gcggtcaagg acaagggata caccctcgat ctggccaagg cacagcagct
     4081 ctgggattcg gccggtgtcg gcgagcagga aatcaccgtc tcgtatgaca ctgacaccgt
     4141 ggctccgggc ggcgcaagct acgagacgat cgcggtcaag gtcaagtcgg acctcgaaca
     4201 gatcaagggg ctcaagatca agctggctcc cgcgcccggt gacgaacgat tggccgccta
     4261 ccgagcaggc gacttccagg cgacactctc gccctggaca ccggactatc cggatgtcga
     4321 ctcctattgc ggaccgtttg cgcgaagcaa cacggctgcc gccaagcgcg ttgggtactc
     4381 ggatcccgag gtcgacaagt tgcttgatca gggcctgtcc gagctcgacc cgaccaagcg
     4441 cgaggcaatc tacgtacaga ttcaggagaa gatgatcgac gcggccgcgt tccatgtgct
     4501 gtaccagccg gtcgatcaga aagccgcgcg agcagttgta cagggagcgg catgccatcc
     4561 cgtgtaccag ctccagctgc gattcgcgag caagaccggt taatccgcga tcttcgatta
     4621 aggtgagtca ggagaccgac acaaatgggg tctcctgatc tcccctttca cacgattatc
     4681 tgtaaggagc gcccgcagtg ggtctctggc aattcatcgc ccggcgcata atccagtcgg
     4741 tcgtggtgct gttcttcgtg agcgttgcca cgttcctgct gatgcatgcc gtgcccggtg
     4801 atcccctggt cgcggtcatc ggggagcgtc aggcggaccg gccggaaatt cgcgcggcgc
     4861 tcgaaaagcg gtactcgctc gacaaaccac tgcccgttca gtacatcagc tatgtccgca
     4921 acatcatcgt caacggggac atgggctcga ccatcacgac ccggcgaccg gtggccgatg
     4981 agctcgccaa gttcattccc ttcaccatcg agctcagcat tgcggcgatg atctttgcgc
     5041 taagcgtcgg cgtccccctg ggcatcattt ccgcgctccg gcagaacagg tggcaagacc
     5101 atatcgcgcg gaccctgtcg ctcattggta cggccatgcc ggtgttttgg ctcgggctcc
     5161 ttctgtcata tgtcatctcg tacaagctgc ggtggctgcc acgttcgggg ttcaaggaaa
     5221 ccgggatgaa gatcccggaa caccataccg gtttcatgac gattgactcg atcatcgccg
     5281 gggactgggc gtttctccat agctatctcc tccacatgat cctgcccgcg attgtgctcg
     5341 gctcattcgc gatgggaatc gtcgccagga tgctcagatc gtcgctcgtc agtgcgctag
     5401 aggacgacta cgttcgcacg gcgcgcgcca agggtctcgg cgagttcagc gtggtgcggg
     5461 gccacgcgct ccgtaatgcg ttgatcccga ctatcacggt cattggcttg acctttgcca
     5521 gtctgctcgc gggcgcggtg ctcaccgaaa cgatcttctc gtggcctggt gtcggtcaat
     5581 tcgccgtcca gatggcgttg aaactggact acccgggcct gcttggagtc acgctgttcg
     5641 ttgccgtcgc atatatcttc gtcaacctga tcgtcgacat tttgtacggc ttccttgacc
     5701 cgcgaatccg gaccggctga ggatatggcc gtgtcgacca ccagcgcgat tccaatcact
     5761 ggagttgccg acccactgtc cacagtcaag cggcgcacac cggcgggtga tgcgatcagg
     5821 accttgcggc agaggccggt tgccttgttc gggctcggag tcctggtggc ctggatcctg
     5881 ctcggtttgc tcgcgccggt tctgccgctc gaagcgcaaa atgggcagat caagatcgag
     5941 aacaagctcg aatcgccgag ccgagaacac cccttcggca cggatgatgc tggccgggat
     6001 gtcatggcga gagtgatcta tgcggctcgg acttccgtcc cggcaggctt ccttgtcgtg
     6061 ttctgtgcct gcgcaatcgg tgccatggtc ggcgccgcat ccgggtacat cggcgggttt
     6121 atcgacacgg tcatcatgcg tgcatgtgac ggtgtgctgg cgttcccgtc gatcatcctg
     6181 gcgatggcga tcgcggtcat ccggggcgaa ccgagtctcc aaaacgcgat gttcgcggtc
     6241 gtgctcgtgc tttggcctga atacgcccgg ctgatgcgtg gccaagtcct cagcatcagg
     6301 cataacgact acgtcatggc cgccgagtca ttaggcgcca ctcggcgtcg cgtgctcttc
     6361 cggcacatca tgccggctac ggacgcaccg atcgtggtaa aggcgaccct cgatgtcggc
     6421 gcggcaatcg tgctgacagc cggtctcagt ttcatcggcc tcggcgccgt gccgccaacc
     6481 gccgaatggg gctcgatggt cagccaggca tccaagaccg gcgttcagta ctggtggtat
     6541 cacaccttcc caggcctcgc gatcgtcagc gtcgtcatgg ccctcaattt cttcggcgac
     6601 ggtcttcgcg acgccctcga tcccaagcgt cgagcccggt gagcatgatc gatccgaatc
     6661 tgaccgggcg gccccgagtc gaggctgcgc tcgaaattct gggaatcgaa gccgcgatcg
     6721 tctcgcccgg cacgcccatg ccgacggtgc cactcgccgc cgaagctgtc ggctgcacgg
     6781 tcgaccagat catcaagacc gtcgtcttcg tcacgaaaga ccggcatcca gtcgtggcgg
     6841 tcgccaacgg gacgaatcgg atcgatcgaa aacggttggc ggcggcgatc ggcgtctcca
     6901 cgctgaagct cgccgatccc gagttcgtgc tcgatgtgac tggctacccg gcgggcggcg
     6961 tctccccggt cggaatccgc acactagatg caccgatcat catcgacggt gccgtgctcg
     7021 agcaggactc ggtttacggc ggagcaggaa ccgaggacga cctgctccac gtcgcaaccc
     7081 gcgatttggt cgcggtgacc ggcggtcgtg tctgcgccat aacgcgcggt gattagccga
     7141 tttctgccgg tctggccgat aattggccgg cccgcatgat gacgggcacc cgaatttcca
     7201 cggaaagtac gagtcgtgac aacgaaactg gtgattctcg gcctgccatc gtccggcaag
     7261 acgaccgtgt tcaacgcgct gacccggtcc gacgcgccga ccggcggctt ctcgtcggcg
     7321 acggatgagc ccaatctggc aacagtcaag gtgccggatc cgcgcctgga tctcttgacc
     7381 gagatgttca agccgcagcg caaggtccag gccgacgtcc agtacctcga cgtcgcggga
     7441 gtcgccaagg gcatggccga gaagggaatg agcgggcagt tgctcggtca cctcgcgcag
     7501 gccgacgcgc tggtccacgt cgtccgcgcc ttcgacgatc cgaacctgcc gcaccccgag
     7561 ggctcagtcg atccagcccg cgacatcgag accatcaacc tcgagttggc cttcagcgac
     7621 ctggcaatca tcgagaagcg gctgcagcgg atcgccgcgc agcttccgaa gatgcgtggg
     7681 gctgaacggg aggcgtacga gcgcgagcag gctgcgatgg agacactcaa gcaagcgctc
     7741 gaaaacgaga cgccgctccg cgacgtggtc gatgcgctcg atccggaaga ttacaagctt
     7801 ctgcgagggt tcggtctatt gacagccaag ccgttgctcg tcctcgtgaa catcggtgag
     7861 gaccagatcg gtgaggctga gcaaatcgag cagcggctgt ccgagcagtt ctcccggtcc
     7921 ggagtccagg tcgccgccct ggccggcaag atcgagtccg agatcgccgc actcgacgac
     7981 gatgatgccg cgacctttat ggaggatctc ggcatcacgg aatcgagccg cgaccgcgtg
     8041 atccggctct ccttctcgct gctcggcctg atgccgttct tcaccgtcgg gcccgacgaa
     8101 tgccgcgcat ggaccgtcca ccgcggcgat tcggccgtcg aggcggccgg cgcaatccac
     8161 accgacatcc agcgcggctt catccgggcc gaagtggtca gctacgacga cctgatctca
     8221 gcgggctcga tggccgagac caaaaagcac ggcacgttcc gccgcgaggg gaagacgtat
     8281 gtcgttcagg acggcgacat catcaacttc ctcttcaacg tctaggacag ccatgagcga
     8341 ctacgaggac gatgcgctcg acatctacct gcgggaaccg gacgacatcg gcgcccgcat
     8401 cgtcctgctg accgcgctct cgatctggcc cgagctaggc gccagcgagg aacagcgaac
     8461 atggcgacgt tggctggatg cacaaggtgt cctgcaaatg gccacgcgag acgaggaaga
     8521 agcgctcgcc ggtccagtgc aaagtgagcg aatcgctgac ctgtgcgagc gcgccgtcga
     8581 cgcactaccc gcgctcgcct gggctacggc cctgatcgac gagattccgc tcatcgaacc
     8641 cgaagacgag cgggccgagt tgatcgagcg cattcctgtt ccggat
//
LOCUS       DATAOZ010000613        12975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8920492, whole genome shotgun sequence.
ACCESSION   DATAOZ010000613 DATAOZ010000000
VERSION     DATAOZ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12975
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8920492"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VHR64_15300"
     CDS             <1..311
                     /locus_tag="VHR64_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304253.1"
                     /translation="RLDINKGVFVINDVRLQGIRTAAFLGDDITDLDGFRALRVLTES
                     GELLASASVGVRAPESPARLLVESDVLVDGVTGMVAELQLFAAELSKWSPTWETDSSR
                     "
     gene            287..787
                     /locus_tag="VHR64_15305"
     CDS             287..787
                     /locus_tag="VHR64_15305"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX3304254.1"
                     /translation="MGNRLVTLILAALLLAPVAVSAKPAVPSWRDHHPWSTEVATVTV
                     GTDELQAEIADTPQLQGRGLGYRDGLDPGTAMLFVFDNASMRTFWMKGMRFCLDIVWI
                     EGGMIVGAAEAVCPMPGTPDAELPTYSSPVGVRYVLEVPAGWLATHRYGAGTPVEINL
                     SDPPAD"
     gene            complement(759..1433)
                     /locus_tag="VHR64_15310"
     CDS             complement(759..1433)
                     /locus_tag="VHR64_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEX3304255.1"
                     /translation="MATDLEQVAASIIERLTTISEVRDRALGDCRQVVRWSANSVRAS
                     HRGDFDEAARELDEARSLAQSVFAYARDVPSVYWAGYVQDAMKEYAEAAITNAIIRNR
                     DIPTPSDLGVEDAAYLNALAEAASELRRDTLDLLRGDELDRAVELLATMDEIYATLVS
                     IDFPDAITGGLRRTTDSLRAVLERTRGDLTVTINQRRLERAVRSMQGSLGLPQTELSQ
                     PEDPKG"
     gene            1549..1624
                     /locus_tag="VHR64_15315"
     tRNA            1549..1624
                     /locus_tag="VHR64_15315"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1582..1584,aa:Ala,seq:ggc)
     gene            1726..4209
                     /gene="leuS"
                     /locus_tag="VHR64_15320"
     CDS             1726..4209
                     /gene="leuS"
                     /locus_tag="VHR64_15320"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX3304256.1"
                     /translation="MVSPTNREQDTGRFDATSIDTKWQAKWAADRLYKTPDESDKPNW
                     YSLTMYPYPSGILHVGHWYAFVAPDAFARFKRRQGFNVLFPMGFDSFGLPAENAAIKG
                     NVHPSISTNRNIEHMRKQYVHMGASIDWDRQISTCDPDYYRWNQWIFLRMLEKGLAYR
                     KMGAVWWCPNDQTVLANEQVLEGNVCERCGSEVYKRDLEQWYFRITEYVDELLAGLDT
                     VDWPERVKTMQRNWIGRSEGARLIFRTESAEPLEVFTTRPDTVWGATFMVLAPEHPLV
                     DVVTTDEHRADVDAYIEQARRKSEIERLSTDESRPKSGVFTGGYAINPVNDERIPIWI
                     ADYVLMGYGTGAIMAVPYGDQRDFDFARAFDLPIRAIVEPEDGSPVDPADRTEAYHDP
                     GTMINSGPFTGKHTPEIIPDVISWLERENRGRGEVTYRLRDWLISRQRYWGTPIPVVY
                     CDDCGIVPVPDSDLPVLLPLDAEFKPTGQSPLVSHQEFLNVACPKCGGPGRRETDTMD
                     TFVDSSWYWYRYLDPHNDALPFDPERIKGWTPVDLYTGGIEHAILHLLYARFFTRVLR
                     DIGQLDQDEPFARLRNQGMILSEEGVKMSKSRGTQVDPDPIVAEHGADTMRLHLMFLG
                     PWDQGGPWNDRGLNGMERFLRRVYDLVRLTTDIGARETTTQDADAIVRMTHRTIKRVT
                     TDLTEFHFNTQIAAMIELTNELMRLRETDLVGTKEWRFAVTTLVALLAPSAPHVAEEM
                     WEMLGQEYSVHTSEWPAWDEALTVDATVEIAIQVNGKPRDRISVPSDATEDAVRELAL
                     ASERVQAHLGGRSPKKVIYVPGRLINIVG"
     gene            complement(4263..5732)
                     /gene="gcvPB"
                     /locus_tag="VHR64_15325"
     CDS             complement(4263..5732)
                     /gene="gcvPB"
                     /locus_tag="VHR64_15325"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX3304257.1"
                     /translation="MAVEPLIFELSRPGRRGVQFPPADVPTTEVPATLRRDHLDWPEV
                     SEIDVVRHFTRVSQKNHAIDINFYPLGSCTMKYNPKINEAVARYSGFASAHPLQDEAT
                     VQGSLELMYDLQEMLANISGFPHVTIQPAAGAQGEFTGVLVMRAYHHSRGDDARTTIL
                     VPDSAHGTNPATAAMVGFKVVTVPSDKRGNVDVDKLRELVGPETAGLMITNPNTLGLW
                     DENIREAIQIVHEAGGLVYNDGANFNAILGMVRPGDLDIDIMHFNLHKTFSTPHGGGG
                     PGSGPVGVRKGLEQFLPGPLVEKRDDGYGWVEPAESVGRLHGFHGNHGMHVRAYTYIR
                     MHGEDGLRQISLDAVLAANYLRVNLTDRYQIWYDRTCMHEFVVSANRQKALGVKTLDI
                     AKRLLDFGVHPPTTYFPLIVPEALMIEPTETESKETLDYFIQAMHKIADEAETNPEIV
                     TTAPHNTEYKRLDDVAANRALNLRWEPERTGELVGAAAD"
     gene            5894..6493
                     /locus_tag="VHR64_15330"
     CDS             5894..6493
                     /locus_tag="VHR64_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304258.1"
                     /translation="MSPYIIFGLAMLGIVIFSLVVTGNLAASFNERAKRDMRASLEPL
                     AELLDGTVDVDEAKVEGRYHGQITTGQVVPGPGGMGRLFQTTYIEPAGGTAWKAVVRR
                     PKTEGEEWERTFEGSGEPISPPQDDIFQLLDGLLPYPGWFELKYDPAAGMLTLTRAMQ
                     TRRDLPTPERFALYLDTLERAGGINRATQAEAGVATTPS"
     gene            complement(6457..7449)
                     /locus_tag="VHR64_15335"
     CDS             complement(6457..7449)
                     /locus_tag="VHR64_15335"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3304259.1"
                     /translation="MSRSESFINYIRRKNPWTKRGLVIRGSLALLIILGALLTNRSFL
                     GWGLFTVFAILIVPTGRIRSFLFSFGPYAAVWFIFTALRSISDETPLARTLNLYAPDF
                     ERELFRGQLPTVTLQDRFFDPNHLHWYDYFCTGIHWSYFIIPHALAIYLWYKRPELFR
                     HFLGAMILLLGMGLAIYFLIPSNPPWLAPEPINSPSAPVIYRVMESIGKQIGGGLYTA
                     SYKVIGESNPRAAMPSIHMAITFLLVFPSYRLSRKFGTLMLLYAACMGYSLIYLGEHY
                     FIDVTVGCGIAAYGWYGFPRLSELIQIARAPGAKPILTPAPPPHEGVVATPASA"
     gene            complement(7498..8004)
                     /locus_tag="VHR64_15340"
     CDS             complement(7498..8004)
                     /locus_tag="VHR64_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX3304260.1"
                     /translation="MYWTNRHVLICNASHCASKGANDVAGRMRLEVIRRKLDAQILIN
                     NCGTIDLCDIGPNIVVYPDNVILSGVKLKDVGRVVDYLAGGDVPEDLVLNRTTPTEKA
                     RRTFYAEAVAMDSPDEAAFAAAADEHGFDGNWIAEQARRGFIARKADPEGILRISVTS
                     KARTRYGV"
     gene            complement(8015..8305)
                     /locus_tag="VHR64_15345"
     CDS             complement(8015..8305)
                     /locus_tag="VHR64_15345"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX3304261.1"
                     /translation="MRKEDRRAYVLHPETADLLHRHMDAGSLVPALNDRDICLHCRRH
                     QCIGTCPTDALETRSDGRLEIHESRCVSCGACVIACFEFVNITWRPRVGTES"
     gene            complement(8302..9435)
                     /locus_tag="VHR64_15350"
     CDS             complement(8302..9435)
                     /locus_tag="VHR64_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HEX3304262.1"
                     /translation="MRISRARWVRLSLILLANALIFPFWIASGAAQSGRLVSVESSTA
                     TSNFPDDMTFELSASVQGKIRKVDLVYQESSLATLELLPGDFQQDYNQLTATAKADLA
                     TYYVPAGIDLTYHWVVTFDDDAVQETDASVVTWIDNRFEWDKREGAGVEVYSYDRSDK
                     FMDFVMETADKAVQDMIALYNPPKVLPIRIWLYESGKDFAGTQAANSQEWAAGAAYPS
                     LQVILGVIPKNSKSEVNRILPHEISHQILAQATINPYNAPATWIDEGLAVVAQIGGKD
                     HYEGDVRDAYHKGELISLRSLISSFPYDPSQASLAYGESYLVMQFVLDQYGPESIQAI
                     IAAYRDGNSQDEVIEKALGMSIEELEQAWLDQFGQSSLDGLAA"
     gene            complement(9383..10496)
                     /gene="prfB"
                     /locus_tag="VHR64_15355"
                     /pseudo
     CDS             complement(9383..10496)
                     /gene="prfB"
                     /locus_tag="VHR64_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922581.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            10577..11785
                     /locus_tag="VHR64_15360"
     CDS             10577..11785
                     /locus_tag="VHR64_15360"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3304263.1"
                     /translation="MLTMLRNRALSAFMMGHFTNDLFMGVLTALLPILKSEYGLSNAE
                     IGLIALVQSGTGSILQPFFGIVADKIRLRWFVPAIILWDSIFVSLFGFARNEQQLLIL
                     AALLGIGSAAFHPYGASGAAHAAEARALNSSLSIYTVAGTSGWAIGPLIATALIALFG
                     PHGTIGFVVIGIGMSLVIASRMTAPVRHEHASRSGTSVDADPVQWGRLSAIIGIVMLR
                     SWVFLAFLSLLPIHYKELGYRLGFYNLLPTIMILSGAVGTLIGGRLADQVPGRRIIIG
                     SQLLSIIPALVAAQVDSAWVMPAAALFGMLSDSSLSITLLAAQRLLPGRTGLASGVIL
                     GLGFVSGSLGVPITGALADRTSIPTALTLSLVLAVAAAIVTAVLPASLFDLARREPRS
                     MARQPSEATA"
     gene            11782..12120
                     /locus_tag="VHR64_15365"
     CDS             11782..12120
                     /locus_tag="VHR64_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304264.1"
                     /translation="MRFGRVQGHGRLVTDGGEPMVSLAVMLDSSSSVTVVRDEAVPWL
                     AFGPDGDVVWQIDQLTQETIGTTLAEEGWEPFSEDESARGETGDGLSHSSVYVLRNMS
                     SSFGYGGEVG"
     gene            12117..12797
                     /locus_tag="VHR64_15370"
     CDS             12117..12797
                     /locus_tag="VHR64_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304265.1"
                     /translation="MRRVGLIICVAIILIQLGLLGYAATRASSQSLVQAPIRPTTQPP
                     VPLDLEEIWPFASSVATNWRSDASLVRASMQMDWPGEAGSPPAGQLPIGGWIFMTFLS
                     SDGLMTMRIDRGSGVIVDTEVLDIDAKFRDAFSTTPIDFSAARTSSGTAASAAEVAYG
                     STFRSACPDLRHTSWIAVQVDPATGATSWHIEYESRGDQPQPSMAMDVDWQTADIRNV
                     ENATEPCP"
     gene            complement(12794..>12975)
                     /locus_tag="VHR64_15375"
     CDS             complement(12794..>12975)
                     /locus_tag="VHR64_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304266.1"
                     /translation="PRSDDRLSWQHADRLQRALQTKVAVKRDASTGQGSITIHFFDDE
                     QLSSLLERLAGEEHF"
BASE COUNT         2329 a         3928 c         4165 g         2553 t
ORIGIN      
        1 cacgcctgga catcaacaag ggcgtgtttg ttatcaacga tgtccgcctg caaggaatcc
       61 ggaccgcagc ctttctcggg gatgacatca cggacctgga cggctttcgc gcacttcgtg
      121 tcctgaccga gtccggcgaa ttgttggcca gtgcgtcggt tggcgtacgg gcccctgaaa
      181 gtccggcacg gctattggtc gaatccgacg tcctggttga cggagtaacg ggcatggtcg
      241 cggagttgca gctgttcgcc gcggagttgt cgaaatggag tccgacgtgg gaaaccgact
      301 cgtcacgctg atcctggcag ctttgctgct cgctccggtc gccgtatccg cgaaaccggc
      361 cgtgccgtcg tggcgggacc accatccgtg gtcgaccgag gtggcgacgg tgaccgtcgg
      421 gaccgatgag cttcaggccg aaattgccga tacaccgcag ctgcaagggc gcggacttgg
      481 ttatcgcgat ggtctcgatc cggggaccgc catgctcttc gtgtttgata acgcctcgat
      541 gcggacgttc tggatgaagg gaatgcgctt ctgcctcgac atcgtctgga tcgagggcgg
      601 aatgatcgtc ggcgcggccg aagcggtctg ccccatgccc ggcacgcctg atgcagaatt
      661 gccgacttac tcgtcgccgg tcggagtgcg gtatgtcctt gaggtcccgg ctggatggct
      721 ggcaacccat agatatggcg caggcacgcc ggtcgagatc aacctttcgg atcctccggc
      781 tgactgagct cggtctgcgg cagcccgagc gatccctgca tcgaccgcac cgcccgctcc
      841 aggcggcgct ggtttatcgt gaccgtcagg tcgccacgag tccgctccag caccgcccgc
      901 agactgtcgg ttgtccggcg caacccgccc gtgatggcgt ccgggaagtc gatcgagacc
      961 agggtcgcgt agatctcgtc catggtcgca agtaactcga cggcgcggtc cagttcgtct
     1021 ccccggagga gatccagcgt atcgcggcgg agctcgctcg cggcttcggc gagcgcgttc
     1081 aggtaggccg catcctcgac gccaagatcg gacggtgtcg gaatatcgcg attccggatg
     1141 atcgcgttgg tgatcgccgc ctccgcatac tccttcatgg cgtcctggac gtagccagcc
     1201 caatagacgc tcgggacatc ccgggcgtat gcgaagaccg attgcgcgag cgaccgggcc
     1261 tcatcgagct cgcgggcggc ttcatcgaaa tcaccccgat gcgatgctct gaccgagttg
     1321 gcggaccagc ggacgacctg ccggcaatcg cccagggcgc gatcgcgcac ctcgcttatg
     1381 gtcgtcagtc gttcgatgat gctggcggca acctgttcga gatccgtggc caatcgtcct
     1441 ctccctgcgc aatggcgagc ccaatttcat tgacatgata cgagcgcaat tggtaggctt
     1501 cttcgactcg agcgctggtc acagcggatc gaaccatgca ttgatgtcgg ggctgtagct
     1561 cagctgggag agcgcaacac tggcagtgtt gaggtcaggg gttcgagccc cctcagctcc
     1621 accacgacag gatgtctcga cagccttccg cggccagcgc cgggaaggct gttttgtatt
     1681 gtcatatcag tagcgttcat gagcgccagt aagggacagc gaacgatggt ctcgccaacg
     1741 aatcgggaac aggacaccgg ccgcttcgat gccacctcaa tagacaccaa gtggcaggca
     1801 aaatgggccg cggaccggct ctacaagacg ccagacgagt cggataagcc gaactggtat
     1861 tcgctgacca tgtacccgta tccctccggc atccttcatg tcggtcactg gtacgcgttc
     1921 gtcgcgcccg acgcctttgc ccgcttcaag cgacggcagg gcttcaacgt gctcttcccg
     1981 atgggcttcg attctttcgg gctaccggcc gaaaatgcgg cgatcaaggg caatgttcat
     2041 ccgtccatct cgacgaatcg aaacatcgag catatgcgga agcagtatgt gcacatgggg
     2101 gcgagcatcg attgggatcg gcagattagc acctgtgatc ccgattacta ccgctggaac
     2161 cagtggattt tcctgcgcat gctggagaag ggactggcgt atcggaaaat gggtgcggtc
     2221 tggtggtgcc cgaacgatca gacggtgctg gcgaacgagc aggtgcttga aggcaacgtc
     2281 tgcgagcggt gcggttccga ggtctacaag cgtgacctgg agcagtggta cttccggatc
     2341 acggagtacg tggacgagct gctggccggc ctcgatacgg tcgattggcc agagcgcgtc
     2401 aagaccatgc agcgcaactg gatcggtcgc tcggaaggag cccgcctgat ctttcggacg
     2461 gagtcggctg agccgctcga agtcttcacg acgcgtcctg ataccgtctg gggtgcgaca
     2521 ttcatggtcc tggcgcctga gcacccgctc gtcgatgtcg tgacgaccga tgagcaccgg
     2581 gcagacgttg acgcctatat cgagcaggcc cgacgcaaga gcgagatcga gcggctctcg
     2641 accgacgaat cccggccgaa gagtggcgtc tttaccggcg gctacgcgat caatcctgtg
     2701 aacgatgagc ggatcccgat ctggatcgcc gactacgtcc tcatgggata cgggaccggc
     2761 gcgatcatgg cggtgccgta tggcgaccag cgcgacttcg atttcgcgcg ggcgttcgat
     2821 ctgccgatcc gcgcgatcgt tgagcccgag gatggctcgc cggtcgatcc ggccgaccgc
     2881 accgaggcgt atcacgaccc aggcacgatg atcaactccg gtccgttcac tggcaaacat
     2941 acgccggaaa tcatcccgga tgtgatctcg tggctggagc gggagaatcg cggtcggggt
     3001 gaggtcacct accgtctgcg tgactggctg atctctcgcc agcgttactg gggtacgccg
     3061 attccggtgg tctactgcga tgactgcggg atcgtcccgg tgccggattc agatcttccg
     3121 gttctgctgc cgcttgacgc cgagttcaag ccgactggcc aaagcccgct tgtctcccac
     3181 caggaattcc tgaacgttgc ctgcccgaag tgcggcggtc cgggtcggcg cgagacggac
     3241 acgatggaca cgttcgtgga ttcatcctgg tactggtatc gctacctgga tccccacaac
     3301 gatgcgcttc cgttcgatcc cgagcggatc aagggatgga ctccggtcga tctttacacc
     3361 ggcgggatcg agcacgcgat cctgcacttg ctgtacgcgc gcttcttcac ccgcgtgctg
     3421 cgggacatcg gccagcttga ccaggacgag ccgttcgcca ggctgcgcaa ccagggcatg
     3481 atcctctcgg aagagggggt caagatgagc aagagccggg gcacccaggt cgatcccgat
     3541 ccgattgtgg ccgagcatgg tgccgacacg atgcggctgc acctgatgtt cctcggtccg
     3601 tgggatcagg gcgggccgtg gaatgatcgc ggcctgaacg gtatggagcg gttcctgcgg
     3661 cgcgtctacg acttggtgcg cctgacgact gatatcggcg cccgggaaac cacgacccag
     3721 gatgcggatg caatcgtccg tatgactcac cgcacgatca agcgcgtgac gaccgatctg
     3781 accgagtttc atttcaacac gcagatcgcc gccatgatcg agctgacgaa cgagttgatg
     3841 cggcttcggg aaaccgacct cgtcggcacg aaggagtggc ggttcgcggt cacgaccctg
     3901 gtcgcactgc tggcgccgag tgcgccccac gtcgcggaag agatgtggga gatgctgggc
     3961 caggaatatt cggtccacac gtcggagtgg ccggcatggg acgaggcgct gacggtcgat
     4021 gcgacggtcg agatcgcgat ccaggtcaac ggcaagccgc gcgatcgcat cagcgtgcca
     4081 agcgacgcaa ccgaggacgc ggtgcgcgag cttgcgctgg cctcagagcg cgtccaggcg
     4141 catctcggcg gtcgatcacc aaagaaagtg atctacgtgc cgggacgatt gatcaacatc
     4201 gtcggatagc agaacgggct gggacgaatc gtcccagccc gtttcagtcg acgtgaattg
     4261 aattaatcag ccgccgcgcc gaccagctcg cccgtgcgct ccggttccca ccgcaggttc
     4321 agcgcccggt tcgcggcaac gtcatcgaga cgcttgtact cggtgttgtg tggcgccgtg
     4381 gtgacgatct ccgggttggt ctcggcctca tccgcgatct tgtgcatcgc ctggatgaag
     4441 tagtcgagcg tctcctttga ctcagtctcg gtcggctcga tcatcagggc ttccggcacg
     4501 atcagcggga agtaggtggt tggcgggtga acgccgaagt cgaggagccg cttggcgata
     4561 tccagcgttt tgacgccgag ggccttctgc cggttggccg agaccacaaa ctcgtgcatg
     4621 caggtgcggt cgtaccagat ctggtagcgg tcggtcaggt tcacgcgcag gtaattcgct
     4681 gccagcaccg cgtcgaggct gatttggcgc aggccatcct cgccgtgcat gcgaatgtag
     4741 gtgtaggcgc ggacatgcat gccgtggttg ccgtggaatc cgtgcaggcg cccgaccgat
     4801 tccgccggct caacccaccc ataaccgtca tcccgcttct cgacaagcgg cccgggcagg
     4861 aactgctcga gccccttgcg gacgccgacc ggcccggagc ccggaccgcc gccaccgtgc
     4921 ggagtggaga aggtcttgtg caggttgaag tgcatgatgt cgatatcgag gtcgcccggc
     4981 cggaccatgc ctaggatcgc attgaaattc gcgccgtcgt tgtagacgag gccgcccgct
     5041 tcgtggacga tctgaatcgc ctcgcggatg ttctcgtccc agaggccgag tgtgttcggg
     5101 ttggtgatca tcaggccggc cgtttccgga ccgactaact cgcgcagctt gtcgacatcc
     5161 acattgccgc gcttgtccga cgggacggtc acgaccttga agccgaccat cgcggcggtc
     5221 gccgggttgg tgccgtgcgc ggagtccggc acgaggatcg tggttcgcgc gtcatcgcca
     5281 cgcgagtggt gataggcgcg catgaccagc acgccggtga actcgccctg tgccccggcc
     5341 gccggctgga tcgtgacgtg cggaaagccc gagatgttcg cgagcatctc ctgcaggtca
     5401 tacatgagct cgagcgagcc ctgcacagtt gcctcgtcct ggagcgggtg cgcgctcgcg
     5461 aatccagaat agcgcgcgac cgcctcgttg atcttcgggt tgtacttcat cgtgcacgac
     5521 ccgagaggat agaagttgat atcgatcgcg tggttcttct ggctgacgcg ggtaaaatgg
     5581 cgaacgacgt cgatctcgct cacttccggc cagtccagat gatcgcgccg caaagtggca
     5641 ggaacctcgg tggtcggaac atcggccggc ggaaactgga cgccacgccg accggggcgg
     5701 ctcaactcga aaatcagcgg ctcaaccgcc atgcgtgtgg tccccttgtc gaatgaatga
     5761 cggatcaatc gcgtctgggc atcgacgcac cggaattgta cccggattac aagggcggat
     5821 acgatgtacc gacatgccga gccggctggt ttcggcgctg gcgattcctg tttgcaatcg
     5881 ggatctggag tagatgtcac cgtacataat atttggcctg gccatgctgg gcatcgtcat
     5941 tttctcgctc gtcgtcaccg ggaacctcgc ggcgagcttc aacgagcgcg ccaagcggga
     6001 catgcgggca tcgctcgagc cgctcgccga actactggat ggcacggtcg acgtcgacga
     6061 ggcgaaggtc gaagggcggt atcacggtca gataacgacc ggccaggtcg tgcccggtcc
     6121 aggcggcatg ggccgcctgt tccagacgac ctacatcgag cctgccggtg gaacggcctg
     6181 gaaggcggtc gtgcgccggc cgaagactga aggcgaggag tgggaacgga catttgaagg
     6241 ctccggggag cctatttcgc cgccgcaaga cgacatcttc caactcctgg atgggctgtt
     6301 gccgtatccg ggctggttcg agctcaagta cgatccggcc gctggaatgc tcacattgac
     6361 gcgggcgatg caaacgcgcc gcgatctgcc gactcccgag cggttcgccc tttacctgga
     6421 tacgctcgag cgtgccggcg gaatcaatcg cgcgactcaa gccgaggcgg gtgtcgcgac
     6481 cactccttcg tgaggcggag gggcgggggt caggattggt ttcgcgccgg gagcgcgcgc
     6541 aatctggatc aactcggaca ggcgtgggaa tccgtaccag ccatacgcag cgatgccgca
     6601 gccgaccgtc acatcgatga agtagtgctc gccaagatag attagcgagt accccatgca
     6661 ggccgcgtac aagagcatca atgtcccgaa tttgcggctc aaccggtacg aggggaagac
     6721 cagcaggaag gtgattgcca tgtggatcga aggcattgcc gcgcgcgggt tcgattcgcc
     6781 gatcaccttg taactggccg tgtagagacc gccgccgatc tgcttgccga tcgactccat
     6841 gacccggtaa atcacgggcg cggacgggct attgatcggc tcgggcgcca gccagggtgg
     6901 gtttgacggg atcaggaagt agatcgcgag ccccatgcca agcagcagga tcattgcacc
     6961 gaggaaatgc cggaaaagct cgggtcgctt gtaccagaga tagatcgcca gggcgtgcgg
     7021 aatgatgaaa tacgaccagt ggatcccggt gcagaagtag tcataccagt gcagatggtt
     7081 cggatcgaag aatcggtcct ggagcgtgac ggtcggtaac tggccccgga agagttcccg
     7141 ctcaaagtcg ggagcgtaca ggttgagggt gcgggcgaga ggagtctcgt ccgagatcga
     7201 gcgcagcgcc gtgaagatga accagacggc ggcataggga ccgaacgaga acaggaatga
     7261 ccggattcga ccggtcggca cgatcagaat cgcaaagacg gtgaagaggc cccaaccgag
     7321 gaacgaccgg ttggtcagca acgcaccgag gatgatcagg agggcgagtg agcctcggat
     7381 tacgaggcca cgcttggtcc acgggttctt tcgcctgatg tagttgatga acgattcgga
     7441 gcggctcaag gtatttgccc gtattcgcgg ttagtctgtg atcgctcggg cacaacatta
     7501 caccccgtag cgagtccgtg ccttgcttgt gacactaatt cgcaggatgc cctctggatc
     7561 cgctttgcga gcgatgaagc cgcgccgcgc ctgctcggca atccaattcc cgtcgaatcc
     7621 atgctcgtca gcggcagcag cgaatgccgc ctcgtccgga ctgtccatgg cgaccgcttc
     7681 cgcatagaac gtccgccgag ccttctcggt cggcgtagtg cggttcaaca ccagatcttc
     7741 gggaacatcg ccaccggcga ggtaatcgac aacccggccg acgtccttga gtttgacgcc
     7801 gctgaggatc acgttgtcgg ggtagacgac gatgttcggt ccgatgtcgc acaggtcgat
     7861 cgtgccgcag ttgttgatca ggatttgcgc gtcgagtttc cggcggatga cctcgagacg
     7921 catgcggccc gcaacatcat tcgcgccctt cgacgcgcaa tgcgatgcgt tgcagatcag
     7981 gacgtggcgg ttggtccagt acatcgcgct cccctcagga ttcggtgccg acacgaggcc
     8041 gccaggtgat gttgacgaac tcgaaacagg cgatgacgca cgcgccgcag gaaacgcagc
     8101 ggctctcgtg gatttccagg cgaccgtccg accgcgtctc cagcgcgtca gtgggacagg
     8161 tgccgatgca ttgatgccga cgacagtgca ggcagatgtc gcgatcgttc agggccggca
     8221 ccagcgagcc cgcatccata tgccggtgga ggaggtccgc cgtttcgggg tgcagcacgt
     8281 aggcgcggcg atcttccttc ctcatgcggc cagcccgtca agcgacgact gtccgaactg
     8341 atccagccag gcctgctcca gctcctcgat gctcatgccc agcgccttct cgatcacctc
     8401 gtcctggctg tttccatcac ggtacgcggc aatgattgcc tgaatggact ccggaccgta
     8461 ttgatcgagg acgaactgca tgaccaggta gctctcgccg taggccaggc tggcctgcga
     8521 gggatcgtac gggaacgagg agatcaagct ccgcagcgag atcagttcgc ccttgtggta
     8581 ggcgtcccgg acatctcctt catagtggtc cttgccgccg atctgggcca cgaccgccaa
     8641 cccctcatcg atccaggttg ccggcgcgtt gtatgggttg atggtcgcct gcgccaggat
     8701 ctggtgactg atttcgtgcg gcaggatgcg gttcacttcc gacttgctgt tctttggtat
     8761 gacccccagg atgacttgca ggctcggata ggcggcgccc gcggcccatt cctgactatt
     8821 cgctgcctgc gtgccggcaa agtccttgcc ggattcatag agccagatcc gaatcggcag
     8881 aaccttcggc gggttataga gcgcgatcat gtcctgaacc gccttgtcag ccgtttccat
     8941 gacgaagtcc atgaacttgt cggagcgatc gtaggaatag acctcgacac ccgcgccttc
     9001 gcgcttgtcc cattcgaagc ggttgtcgat ccaggtcacg acggatgcgt cggtctcctg
     9061 cacggcgtca tcgtcgaagg tgacgaccca gtggtaggtc aggtcgatcc ctgccggcac
     9121 gtagtacgtc gccaggtcgg ccttggcggt ggcggtcaat tgattgtagt cctgctggaa
     9181 atcgccgggc agaagttcga gtgtcgcgag gctcgattcc tggtagacca gatcgacttt
     9241 cctgatcttc ccctgaactg aggccgaaag ctcgaacgtc atgtcgtccg ggaagttcga
     9301 cgtcgccgtc gacgactcga cagagaccaa tctgcccgat tgtgccgcac cgctcgcgat
     9361 ccagaacggg aagatcaggg cgttagccaa taggatcagg ctgagcctga cccatctggc
     9421 tcttgagata cgcatcaata aataccccga tctcaccatt cagaacagcc tgagtatcgc
     9481 caacgctgac atctgtgcgg tgatccgtaa ccatggtgta gggatgaaga acgtaggagc
     9541 ggatacggtt gccgaagccg gccgccgtgt gctcgccctt gagtctcgag cgctcctcgg
     9601 cttctgcgct catcttgcgc tcgaggaggc gagcccgcag gatcttccac gccgtctcgc
     9661 ggttctgaat ctgactccgc tcgttctggc aggtgacgac gatgccggtc ggctcgtggg
     9721 tcaggcggac tgctgacgat gtcttgttga cgtgctgacc accggcgcca ctggcccggt
     9781 aggtgtcgac gcggacgtcg tccggattga gatcgatttc ggtgtcgctc tcgatctctg
     9841 gcagcacctc aaccagtgcg aaggaggtat gccggcgatg ggctgagtcg aagggggaca
     9901 aacgcacgag ccggtgcgac ccggcctcgg ccttggcgta accatacgcg ttcgacccgg
     9961 tgatttcgag cgtgccgttc ttgatgccgg cttcctcgcc ctcggtcgta tcgagcactt
    10021 cgctcttgta cccggcttga ccggcccact tgacatacat gcgcagcagc atctcggccc
    10081 aatcctgggc gtcgatcccg ccttcgccgg agtggactac cagtatggcg ttggactggt
    10141 cgtgcgggcc ggagagcgtc gcggccagct ccatttcatc agtcagcgca gtcaatcggt
    10201 tgagctcggc ccggaaatcc tcctcgaggc tctcgtcgtc ggcgaggtcg atcagctcac
    10261 cgagatctgt gagcgaccga tcgagttctt gccactgctc gatgcgggac cggagctcgc
    10321 cgatccggcg catcacgcgc tgcgcgttat cggtgtcatt ccagaagccg tcgatcccgc
    10381 tctggtgttc gagatcggcg agctcgcgct cattattggc gaagtcaaag tcgcaccccg
    10441 agcagatcag cccgctcgcg gagtgccgcg acctctcttc ttaacgtgtc cagcatgggg
    10501 tctcccgtca gcaacaaata tgtgtgagta tacccgggcc aaatcgggcg atcggccatt
    10561 ttcctgctgg attccaatgc tgaccatgct tcgaaatcgc gcgctgagcg ccttcatgat
    10621 gggtcatttc accaatgacc tcttcatggg cgtgctaacc gcgcttttgc cgattctcaa
    10681 gtccgaatac ggcttgagca atgccgagat cgggctgatc gcgctggtcc agagcggcac
    10741 gggctcgatt ctgcagccgt tcttcgggat cgtcgccgac aagatccggc tccgctggtt
    10801 cgtcccggcg attatcctct gggactcgat cttcgtctcg ctttttggat ttgcccggaa
    10861 cgagcagcag ttgctgatcc tggctgccct gctcggcatc gggtcggcgg cgtttcatcc
    10921 gtacggggca tcgggcgctg cgcatgcagc cgaggcacgc gcgctcaact cgtcgctctc
    10981 gatctacacc gttgccggta cgagcggctg ggccattggt ccattgatcg cgactgccct
    11041 gatcgccctg ttcggtccgc atggcacgat cgggttcgtg gtgatcggta tcggcatgtc
    11101 gctcgtgatc gcgagccgga tgaccgcacc ggtccgtcac gagcacgctt cgcgtagtgg
    11161 gacgtcagtg gatgcggatc ccgtccagtg gggccgattg tccgccatca tcgggattgt
    11221 gatgttgcgg tcgtgggtct tcctggcctt tctcagcctg ctgccgattc actacaagga
    11281 gctgggctac cgcctcgggt tctacaatct cctgccgacg atcatgatcc tgtccggcgc
    11341 cgtcggaacg ctgatcggcg gtcgactcgc cgatcaggtg cccggtcggc ggatcatcat
    11401 cggctcgcag ctcctctcga tcatcccggc actggtggcc gctcaggtcg atagcgcgtg
    11461 ggtaatgccg gcggccgccc tcttcggcat gctgtccgac tcgagcctca gcatcacgct
    11521 ccttgccgcg caacggctgc tgcctggtcg aacgggcctc gcatcgggcg tgatcctcgg
    11581 gcttggcttc gtatctggtt cgctgggcgt gccgatcacc ggtgcgctgg ccgaccggac
    11641 cagcatcccg accgcattga ctctctcgct cgtcctggcc gttgcggccg cgatcgtcac
    11701 ggccgtgctg ccagcatcgc tgttcgatct tgctcggcgc gagccccgct ctatggcccg
    11761 gcaaccctcg gaggcgacgg catgagattc gggcgcgttc agggacacgg ccgactcgtt
    11821 accgatggcg gcgagccgat ggtgtcgctc gccgtgatgc tcgattccag ctcatctgta
    11881 acggtggtgc gggacgaagc ggttccatgg ctcgcattcg ggccggatgg ggatgtcgtg
    11941 tggcagattg accagttgac gcaggagacg attggcacca cgctggcaga agagggatgg
    12001 gaaccgttct ctgaggatga atcggctcgc ggcgagaccg gcgatggtct cagccattcc
    12061 tcggtttatg tgctgcggaa catgagcagc tcgttcgggt atggaggcga ggtcggatga
    12121 ggcgggtcgg actgatcatc tgcgtcgcga tcatcctgat ccagcttggg ctactcggtt
    12181 atgccgccac ccgcgcgagc tcgcaaagtc tcgttcaggc cccgattcgg ccaacgaccc
    12241 agccgccagt gccgctggac ctggaagaga tttggccgtt tgccagcagt gtggcgacca
    12301 actggcgatc agacgcatcg ctcgtccggg ccagcatgca aatggactgg ccgggcgagg
    12361 cggggtctcc accggccggt cagttgccga tcggcggctg gatcttcatg acattcctga
    12421 gcagcgacgg cctgatgacg atgcggatcg accggggatc gggcgtgatc gtcgacaccg
    12481 aggtgctcga cattgatgca aagttccgcg acgcgttctc taccacgcca atcgactttt
    12541 ctgccgccag gacatcgtcg ggcacagctg ccagcgccgc ggaggtcgcg tatggctcta
    12601 cgtttcgttc tgcgtgtccc gatctgaggc acacgtcctg gatcgccgtc caggttgatc
    12661 ccgcgaccgg tgccacgtcc tggcacatcg agtacgagtc ccggggagac cagccgcaac
    12721 cgtcgatggc tatggacgtc gactggcaga cggccgatat ccgaaatgtc gagaacgcga
    12781 cggagccctg cccctagaag tgctcttccc cggccagacg ctcgagcagg ctcgagagct
    12841 gctcatcgtc gaagaagtgg atggtgatcg atccctggcc cgttgacgca tcgcgcttga
    12901 ccgcaacctt ggtctgaagc gcgcgctgga gccgatcggc gtgctgccag ctcaggcgat
    12961 cgtcgcttcg agggt
//
LOCUS       DATAOZ010000614         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3658425, whole genome shotgun sequence.
ACCESSION   DATAOZ010000614 DATAOZ010000000
VERSION     DATAOZ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3658425"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VHR64_15380"
     CDS             <1..214
                     /locus_tag="VHR64_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304267.1"
                     /translation="RVLIANFGSASNVLVAEIAGAASGEADMPRLFFCSTDERYGGIG
                     YPPELTEQNGEPAICIPARSGVIYAV"
     gene            complement(404..1354)
                     /locus_tag="VHR64_15385"
     CDS             complement(404..1354)
                     /locus_tag="VHR64_15385"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX3304268.1"
                     /translation="MAVKTSMLDYAYFEGEIVPFGEANISIGTHAIQYGTGAFAGIRG
                     YLDQDGETINIFRLVDHTTRLLNSGKLLRAELPYTPESLAEVIASLVAKNTPTTDIYI
                     RPFIYKPAVQLTPRLRGLGDEVAVYMMGMGDYLDLDRGQKAAVSSWVRVPDNAIPSRG
                     KLTGAYINSAFIKDEAEEKGADEAIVLNTSGKVSEGSGCNLFMVRNGVLTTPPLTADI
                     LEGITRRSILQFAQEAGIPTEQREIDRTELYLAEEAFFCGTGVQVAWIESIDGRPVGN
                     GKLGPISAQIRDMFFDTVRGKRAEHKDWLYQVKKAVPATV"
     gene            complement(1454..2215)
                     /locus_tag="VHR64_15390"
     CDS             complement(1454..2215)
                     /locus_tag="VHR64_15390"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX3304269.1"
                     /translation="MSDKKWDWRTDSVLAKGPARVAVIAAHPDDPDHGCAGACAAWSD
                     MGHHVTYVIVTNGSKGSDDVHLSEADLIATREREQRAAAEILGLKGGVVFLGKEDGMV
                     VPDLELRRDIVRVIRKLKPDVILCENPVSWFYGESYINHPDHRAVAQATLEAIFPASQ
                     NHRYFPELLAEGHEPHRVREVWLDSFGDTEDAVVDVTPVVDRKVAALRAHKSQMLDHD
                     PDEWIREWMRENGKKHNPPVEYAESFKVMRINGEE"
BASE COUNT          423 a          809 c          812 g          483 t
ORIGIN      
        1 gcgggtcctg atcgccaact tcggcagcgc gtcgaacgtc ctggtcgcgg agatcgccgg
       61 cgcagcgagc ggtgaggccg acatgccccg gttgtttttc tgctcgaccg acgagcgcta
      121 cggcgggatc ggctatcccc ccgagctcac cgaacaaaac ggcgagcctg ccatctgcat
      181 ccccgcccgc agcggtgtga tttacgctgt gtaacccgat ccgaacggag aaaaggacga
      241 aaaaacgtag cgcggcccgc ctctgggccg cgccggtaat ggatgtgatg cttaatggtg
      301 cccgtttggc ttcgtcaacc gcgcgtcccg ccgaccccaa gccggccaca gggggccggc
      361 gctacgggag gtgtgcgacg accgcggctt gccgcgcgtg tgtttagacg gtcgcgggaa
      421 cggccttctt gacctggtag agccaatcct tgtgttcggc gcgcttgccc cggaccgtgt
      481 cgaagaacat gtcgcggatc tgggccgaga tcgggcctag cttgccgttg ccgaccgggc
      541 ggccgtcgat cgattcgatc caggcgacct ggacgccggt accgcagaag aacgcttcct
      601 cggccaggta gagctcggtc cgatcgatct cgcgctgctc ggtcgggatt ccggcctcct
      661 gcgcgaactg gaggatggac cgccgggtga tcccctcgag gatgtcggca gtcagcggcg
      721 gggtcgtcag tacgccgttg cggaccatga acaggttgca gccggagccc tcagagacct
      781 tgccggaggt gttgagaacg atcgcctcgt ccgcgccctt ctcctctgcc tcgtccttga
      841 tgaaagccga gttgatgtaa gcgccggtca acttgccgcg gctcgggatt gcgttgtccg
      901 gcacgcgcac ccacgacgag acggctgcct tctggccgcg atcgaggtcg aggtagtcgc
      961 ccatgcccat catgtagacc gcgacctcgt cgcccaggcc gcgcaggcgc ggggtgagct
     1021 ggacagcagg cttgtagatg aacgggcgga tgtagatatc ggtggtcggc gtgttcttcg
     1081 ccaccagcga ggcgatcact tcggccagcg actccggcgt atagggcagc tcagcacgga
     1141 gcagcttgcc ggagttgagc agccgggtcg tatggtcgac caggcggaag atgttgatcg
     1201 tctcgccgtc ctgatcgaga tagccgcgaa tccccgcgaa tgcgccggtg ccgtactgga
     1261 tggcgtgggt gccaatgctg atattggcct ctccgaacgg gacgatctcg ccctcgaagt
     1321 acgcgtaatc gagcatcgat gtcttgacgg ccatctccgg cttcctttgc ttggcggtga
     1381 tcccggcgct taccgccggt gttttgcctc gaaatccatg tttcggatta ttgcacagcg
     1441 cccctgggcc aggtcactcc tcgccgttga tacgcatcac cttgaacgac tcggcgtact
     1501 cgaccggcgg gttgtgcttc ttgccgttct cgcgcatcca ctcgcggatc cattcgtccg
     1561 ggtcgtggtc cagcatctgg ctcttgtgcg cccgcagcgc cgcgacctta cgatcgacga
     1621 ctggggtgac atcgacgacg gcgtcctcag tgtcgccaaa cgagtccagc cagacttcac
     1681 gcactcgatg cggctcgtgg ccctccgcga gcagctccgg gaagtagcgg tgattctgcg
     1741 aggccgggaa gatcgcttcg agtgtggcct gcgcaacggc gcgatggtca gggtgattga
     1801 tgtagctctc gccgtagaac caggagaccg ggttctcgca gaggatgaca tccggcttga
     1861 gcttgcggat cacacggacg atgtcccgtc gaagctcgag atccggcacg accatgccgt
     1921 cttccttgcc caggaagacg accccgccct tgagtccgag gatctcggcg gcagcccgct
     1981 gctcccgctc gcgcgtggca atgaggtcgg cctcgctgag gtggacatcg tcactgccct
     2041 tgctcccgtt ggtaacgatc acgtaggtga cgtgatgtcc catgtcggac caggccgcgc
     2101 atgccccggc gcagccgtga tcggggtcgt cgggatgcgc ggctatgacc gcaacccgcg
     2161 ccggaccctt cgccaggaca gaatctgttc gccaatccca cttcttgtcg ctcataccct
     2221 caccttgcca acggggccct tgcagcccac cccggttgat tgcaccgatt ccgaattatg
     2281 ggacatggcg cgccaaacca agtcggccgg gggcgcaggg ttgacgtatg cataagatgg
     2341 agttcgtcac ccgacacgga ccgaagacat gagcggagag gaaccacatg acccgcactg
     2401 tcctgttcat tcacggcgcc tggctgaccc ccgccagttg ggacgacttc cgggccgagt
     2461 tcgaaaagcg cggctatcag accgtcgccc cggcgtggcc ctatatggac cgctccgtta
     2521 tggagtt
//
LOCUS       DATAOZ010000615         4629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9446029, whole genome shotgun sequence.
ACCESSION   DATAOZ010000615 DATAOZ010000000
VERSION     DATAOZ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9446029"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VHR64_15395"
     CDS             complement(<1..423)
                     /locus_tag="VHR64_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304270.1"
                     /translation="MWRLKYLIYGMIPSAVLGIALYFLAPEITDAFRDAVRDSASRSV
                     QTAFEREAPSTVAPGRLVITEDELLQAVEDADARERSWNISGMDVVIDGGRVAFVEND
                     HNSSTNDATIASSVPKVVDGRIVLTDRQGVLSIFKPARD"
     gene            694..2028
                     /locus_tag="VHR64_15400"
     CDS             694..2028
                     /locus_tag="VHR64_15400"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX3304271.1"
                     /translation="MKSEAMNQFLADVMSGRQNRRDLFRRAAALGLSATAATSLINLA
                     GAQDASPSPSNLTGDLEVFSWWTSPGEHPALEALFAAYTKSNPNVKIIDAAVAGGGGG
                     PAQAVLQTRLQGNNPPDSWQSHPGHELVDQYVDAGYTETLSDLYASTGLDKAVPQGLT
                     DLCSKDGQQYLVPVGVHRGNGLWYNKDVVSAAGVTVGETMTQDEFMAALDKLKAAGVT
                     GLAIGDKDSFVDAQTFENHLLGALGPDKFVALFSGEAKWDDQEVKDVMTSFTKLLDYV
                     NSDHSALSWSDATAMIIEGKAAFNTMGDWAYGEFVAKKVVDKMGWLSYPGSDGSFVLV
                     VDGFTKPKGAPHMDNAIAWLETCASVDAQAAFNPLKGSIPARTDVPTDGFSEYHKWSI
                     ASFAKDHLVGSAAHGLTTSPAFKQAFYDAAIAFVTDKDVDTFATTLVDAASA"
     gene            2120..3163
                     /locus_tag="VHR64_15405"
     CDS             2120..3163
                     /locus_tag="VHR64_15405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3304272.1"
                     /translation="MATLTTAASVERAAVGVRKRRRRIGERWLSPALLSPSIVAMFIF
                     VYGFIAFTFWVSISNWKTNRRDLSISDPTFKTYNTLFNQTRFQIDLRNTLVFTVLFLI
                     LAVFAGLALALLLDRHVMGSNLFRSIFLFPYAISFITTGVVWRWIFNPETGVNALFDQ
                     TGINDALGKMGVGPLKPGWITDPAVVWQVNGALEKIFPWADVIKANLGIPVAMIPVVI
                     AATWQLSGFAMAMYLAGLGTIPGEIKEAALVDGASDWRAYKDIIIPLLKPITISTMII
                     LGHVSLKIFDLIYAMSGKGPGFATDVPGIFVYDKLFGAQQYNLGSAASIVMLVLVSLV
                     IVPYLWRQLKELT"
     gene            3163..4026
                     /locus_tag="VHR64_15410"
     CDS             3163..4026
                     /locus_tag="VHR64_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3304273.1"
                     /translation="MAADLSGIHAAHPRQKRSFGNIVVFVVLVLAALFYVAPVYVMII
                     NGLKDKSYMTLSDMWKLPRDLSGGGFGYAWDALRPYMLNSLQMAIPATIGSSLLGAIN
                     GYLLSKWKFRGSDILFSLVLFGMFIPYQSILVPLIETLQRFGLYGKWYGLTFVHIIYG
                     IPITTLIFRNYFSNIPSELIEAARIDGAGLIKIFWQIMLPLGLPAFVVVGIFQFTNIW
                     NDFLFGVTVVFSPSAQPVTVALNNLQGTQSVDWTVVMAGAAIAAAPTMIIYVLLGKFF
                     IRGLLAGSMKG"
     gene            4023..>4629
                     /locus_tag="VHR64_15415"
     CDS             4023..>4629
                     /locus_tag="VHR64_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304274.1"
                     /translation="MSRSYDIVIIGTGMGGGTLARALKDSGLKILLLERGPMLPVEPE
                     NQSAAALFIENRYKAKELWIDAKTGAPYKPGVHYWVGGNTKVFGAAFPRLRPQDFDDL
                     EHEGGTSPAWPISYDDLEPYYAIVERWYWVHGEAGTDPTEGWRSSPYPHPPVPADPYM
                     EDLADRMRQNGLHPFPLPIGIDLQTGGKCIRCGTCDAFPCMY"
BASE COUNT          847 a         1443 c         1408 g          931 t
ORIGIN      
        1 atcccgggcc ggcttgaaga tgctcagtac gccctgccgg tcagtcagca caatccggcc
       61 gtcgacgacc ttggggacgg acgacgcgat cgttgcgtcg ttggtcgaac tgttgtggtc
      121 gttctccacg aaggcaactc ggccgccatc gatgacgacg tccatgccgc tgatattcca
      181 gcttcgctcg cgggcatcgg cgtcctcgac cgcctggagc agctcatcct cggtgatcac
      241 gaggcgaccg ggcgcgaccg ttgatggcgc ctcccgctcg aatgcggtct gcacggagcg
      301 agatgcagaa tcgcggaccg cgtcgcgaaa cgcgtcggtg atctcgggcg cgaggaagta
      361 gagagcgatg ccgagcaccg ccgaggggat catgccgtag atcaggtatt tcagtcgcca
      421 cacggtgcgg tctcctccag aagttcaatg tgctggcgct attgtgcaat atggttcgcc
      481 cgcctccaca aacggtgatc gcatcggccg attgcccgtc tgcgggagcg ctcccacgtt
      541 gacgtaaagg tacccacggg ctataactcg gacgaacgcg tgcagtctca tattcgtggc
      601 cggcggggcc attcctgcgg tcaatgtcgc gagattttgc gcgtcgccag tcggtgtcga
      661 tcggcgcgaa cacacaattc aggaggagag atcgtgaagt cagaggcgat gaaccagttc
      721 ctggctgacg tgatgagcgg ccgccaaaat cggcgcgatc tgtttcgacg agccgccgcg
      781 cttggcctca gcgcgactgc cgccactagc ctgatcaacc tcgccggcgc ccaggacgcg
      841 agcccgagcc ccagtaattt gacgggcgac ttagaggtct tctcctggtg gacctccccc
      901 ggtgagcatc cggcgctgga ggcgctcttc gccgcataca caaagtccaa cccgaacgtc
      961 aagatcattg acgcggcagt tgccggaggc ggtggcgggc cggcccaggc cgtgctccaa
     1021 actcgcctgc agggcaacaa cccgccagac tcgtggcagt cgcatcccgg tcacgaattg
     1081 gtcgaccagt acgtcgatgc cgggtacacc gagaccctaa gcgacctcta tgcgtccacc
     1141 gggcttgaca aggcagtgcc gcagggtctg accgacctgt gctccaagga cggccagcag
     1201 tacctcgtcc cggtcggcgt ccatcgcggc aatggcctct ggtacaacaa ggatgtcgtt
     1261 tccgctgcgg gcgtgactgt tggcgaaacc atgacccagg acgagttcat ggcggcgctc
     1321 gacaagctca aggcagcagg agtgaccggg cttgccatcg gcgacaagga ttccttcgtc
     1381 gatgcccaga cgttcgagaa ccacctcctc ggcgcgctcg ggccggacaa gttcgtcgcg
     1441 ctcttcagcg gtgaagccaa gtgggacgat caggaggtca aggacgttat gacctccttc
     1501 accaagctgc tcgactacgt caattcggat cactcagcgc tcagctggtc cgacgcgacg
     1561 gcgatgatca tcgagggcaa ggccgccttc aacacgatgg gcgactgggc atacggtgag
     1621 ttcgtggcca agaaggttgt cgacaagatg gggtggttga gctatccggg ctcggacgga
     1681 tcattcgtcc tcgtcgtgga cggcttcacc aagccgaagg gcgccccgca catggacaac
     1741 gcgatcgcct ggctcgagac ctgtgcttcg gtcgatgccc aggccgcgtt caacccgctc
     1801 aagggctcga tcccggcccg gaccgatgtc ccgaccgatg gcttctcgga gtaccacaag
     1861 tggtcgattg cctcgttcgc caaggatcac ctggtgggga gcgcggcgca tggtctgacg
     1921 acaagcccgg ccttcaagca ggctttctac gacgctgcga tcgccttcgt gaccgacaag
     1981 gatgtcgaca cgttcgcaac cacgttggtc gatgctgcct cggcgtagtt ctgccctcag
     2041 ggttttctga cgttgacaat cggggcgcac gctcgtgcgc cccgattgca tacattcggc
     2101 gtggagcgga tagtggatca tggcaacact gaccaccgca gcatcggttg aacgggccgc
     2161 cgtcggagtg cgcaagcgcc gccgcagaat cggcgagcgc tggctttcgc ccgccttact
     2221 ctctccgtcg atcgtggcaa tgtttatctt cgtctatggg tttattgcat ttacgttctg
     2281 ggtctcgatc tcgaattgga agacaaatcg gcgcgacctt tcgatttctg atccgacatt
     2341 caaaacctac aacacgctct tcaaccagac ccgattccag atcgatctcc gcaacacgct
     2401 tgtcttcacg gtgctgttcc tgatcctggc cgtctttgcc gggctcgcgc tcgcgttgct
     2461 gctggaccgt catgtgatgg gcagcaacct gttccggagc atcttcctct tcccgtatgc
     2521 catctcattc atcacgactg gcgtcgtctg gcgctggatc ttcaaccctg aaacgggcgt
     2581 caacgcgctc ttcgaccaga ctggaatcaa cgatgccctc ggcaagatgg gagtcggacc
     2641 gctcaagccg ggatggatca ccgatccggc ggtcgtctgg caggtcaacg gtgccctcga
     2701 gaagatcttc ccgtgggccg acgtcatcaa ggcaaatctt ggaatcccgg ttgcgatgat
     2761 cccggttgtg atcgcggcga cctggcagct ttctggcttc gcgatggcga tgtacctggc
     2821 cggtctgggc acgatccccg gcgagatcaa ggaggcagcg ctggtcgacg gtgcatcgga
     2881 ctggcgggcg tacaaggaca tcatcattcc actgctcaag ccaatcacga tcagcacgat
     2941 gatcatcctc gggcacgtct cgcttaagat cttcgacctg atctacgcga tgtccggcaa
     3001 aggacccggc ttcgcgaccg atgtgccggg catctttgtc tacgacaagt tgttcggtgc
     3061 gcagcagtac aacctggggt cggcggcatc gatcgtgatg ctggtcctgg tatcgctcgt
     3121 gatcgtgccc tacctgtggc gccagttgaa ggagctgacg taatggctgc tgatctgagc
     3181 ggcatccacg ccgcgcatcc gcgtcagaag cgcagcttcg gcaacatcgt cgtcttcgtg
     3241 gtgcttgtgc tcgcggcact gttctacgta gctccggtct acgtgatgat catcaacggg
     3301 ctcaaggaca agagctacat gaccctctcg gacatgtgga agttaccgcg cgatctctcc
     3361 ggcggcggct tcgggtatgc ctgggatgcg cttcgcccgt atatgctgaa cagcctgcag
     3421 atggcgatcc cggccacgat cggctcgtcg ctgctcggcg cgatcaacgg ctacctgctc
     3481 tcgaagtgga agttccgcgg gtccgacatc ctcttctcgc tggtgctctt cggcatgttc
     3541 attccctacc aaagcattct ggtgccattg atcgagacat tgcagaggtt cggcctgtac
     3601 ggcaagtggt atggattgac gttcgtccac attatctacg gtattccgat cacaacgctg
     3661 atcttccgta attacttctc caatatcccg agcgaattga tcgaggcggc tcggatcgat
     3721 ggggccgggt tgatcaagat cttctggcag atcatgctgc cgcttggctt gcccgccttc
     3781 gtggtggtcg gcatcttcca gttcacgaac atctggaacg acttcctctt cggcgtgacc
     3841 gtcgtcttct cgccctccgc gcagcctgtc actgtcgccc tgaacaacct gcagggcacg
     3901 cagtcggtcg actggactgt cgtaatggcg ggtgcggcga tcgcggcggc gccgacaatg
     3961 atcatctacg tgttgctcgg caagttcttc atccggggcc tgctggcggg atcgatgaag
     4021 ggatgagcag gtcgtacgac atcgtcatca tcggcaccgg gatgggcggc gggacgttgg
     4081 cccgcgccct gaaggacagc gggctgaaga tcctgctgct cgagcgtggg cccatgctgc
     4141 cggtcgagcc ggagaaccag agcgcggcag cactcttcat cgagaaccgg tacaaggcaa
     4201 aagagctctg gatcgacgcc aaaaccggcg cgccctacaa gccgggcgtg cattactggg
     4261 tcggcggcaa caccaaggtc ttcggcgcgg ccttcccgcg tctgcggccg caggatttcg
     4321 acgatctcga gcatgagggc ggcacctcac cggcctggcc gatctcgtac gacgatctcg
     4381 agccgtacta cgcgatcgtc gagcgctggt actgggtgca cggcgaggcc gggaccgacc
     4441 cgaccgaagg ctggcgctcg tcgccgtatc cacatccacc cgtgccggca gacccctaca
     4501 tggaggacct cgccgatcgc atgcgccaga acggcctgca ccctttcccg ctgccaatcg
     4561 gaatcgatct ccagaccggt gggaaatgca tccgctgcgg cacctgcgac gccttcccgt
     4621 gcatgtacg
//
LOCUS       DATAOZ010000616         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6555476, whole genome shotgun sequence.
ACCESSION   DATAOZ010000616 DATAOZ010000000
VERSION     DATAOZ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6555476"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="VHR64_15420"
     CDS             <1..1523
                     /locus_tag="VHR64_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX3304275.1"
                     /translation="DLTGMIRDRRMRTRLPRMLGIYGPGIAHAVDRLLEDPRLATRSG
                     RAIDYTIVIRVSAKLAPGLLVGVVRSLVNPAAARRRAFREADRVEAATLPQPETVGDR
                     IQAAATAQDVVLGGPMTRMLPPLYAGLLSSPIAAALLGPVARPGEVDATQRGMPYNVT
                     TQMDLDLWRVGIAAATYRSVFLQSSPEELAERFHRGDLPEIGLREFLARYGHRGAAEI
                     DVGVPRWADDPTPVFAALAGYLRVEDPQQAAEDRFTRAASEAEETLDRLIGRAMRTRP
                     LRAALAGFLLRRSRELAGLRELPKFVWLYPLREVRRQLLVAGAELTDLGRLHQADDMM
                     FLTLDEAKAAVEGSDQRELAASRRAEHERETKRTRVPGLLLSDGTMPENLPDDSARHD
                     ENTLVGMPAAAGVATGNARIVHDPATARIELGDILVAATTDPGWTRLFLSAGGLVTET
                     GSPIPHGPTVAREYRIPAVICVPTPPTDCTTARKSPSTAAPRPSNSRPLTSRRPGE"
     gene            complement(1564..>1845)
                     /locus_tag="VHR64_15425"
     CDS             complement(1564..>1845)
                     /locus_tag="VHR64_15425"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEX3304276.1"
                     /translation="AGMLALTSAKSGALVGVLISVTTIPAAANAAVALAYWVPSEAIG
                     SAVQLVINLSAIVVAGTLTLLVLRARAEQLARRETSNPQPNEGLVTPSR"
BASE COUNT          321 a          619 c          611 g          294 t
ORIGIN      
        1 tcgacctcac cggcatgatc cgtgatcggc gaatgcggac gcgactgcca aggatgcttg
       61 ggatctacgg acctggcatc gcccacgccg tcgaccggct cctggaggat cctcggcttg
      121 cgacgcggtc gggccgagcg atcgattaca cgattgttat ccgggtcagc gccaagctgg
      181 cacctggact tttggtgggc gtggtgcggt ccttggtcaa tccggccgcg gcccgacgtc
      241 gagcgtttcg ggaagcagac cgcgtcgaag ccgcgacgct gccgcagccg gagacagttg
      301 gagatcggat ccaggcggcc gcgacggccc aggatgtcgt gctcggcggc ccgatgacgc
      361 gaatgctgcc accactctat gccgggctgt tgtcgagccc gattgcggcc gcacttctgg
      421 gtccggttgc ccggcccggc gaggtagacg ccacccagcg cggcatgcca tacaacgtga
      481 ctacccagat ggacctggat ctatggcggg ttgggatcgc cgccgcgaca tatcgctcgg
      541 tcttcctgca aagctcaccg gaggaactgg cggagcgatt ccatcgcggc gatctgccag
      601 agatcgggct gcgcgaattc ctcgcccggt acggtcaccg cggcgcagcc gagatcgatg
      661 ttggagtgcc gcgctgggca gacgacccaa caccggtctt cgccgccctg gccggatacc
      721 tgcgagtcga ggatccccag caggcagcgg aagacaggtt cacacgggct gccagtgagg
      781 cggaggagac actcgatcgg ctgattggcc gagccatgcg tacccggccg ctccgcgccg
      841 cgctagcggg gttcctgctg cgccgctccc gggaactggc cggtctgcgg gaattgccca
      901 agttcgtctg gctgtatccg ttgcgagagg tccgtcggca gcttctggtc gccggcgcgg
      961 aactcaccga ccttggacgg ctgcaccaag ccgacgacat gatgttcctg accttggacg
     1021 aagccaaggc cgccgtcgaa ggatcggacc aacgcgagtt ggcggccagc cgtcgagccg
     1081 agcacgaacg ggagacgaaa cggacccggg tccccggcct gctgctctcc gacggaacca
     1141 tgccggaaaa ccttccagac gactccgcgc gccacgacga gaacactctc gtcggcatgc
     1201 cggccgccgc cggcgtcgcg accggcaatg cccggatcgt ccacgacccg gcgacagcgc
     1261 gcatcgagct gggggacatc ctcgtcgccg ctaccaccga tccaggatgg actcggctgt
     1321 tcctcagcgc aggcgggctg gtcaccgaga ccggttcgcc tatcccgcac ggaccgaccg
     1381 tcgcccgcga ataccggatt cccgccgtga tctgcgtacc gacgccacca accgactgca
     1441 cgacggccag aaaatcaccg tcgacggcag ctccgcgacc gtccaactcg cgacctctga
     1501 cgagccggcg accaggtgaa tgaactgcct tcgggcgttc cgggttcgtg gggaccttga
     1561 agatcaccga ctgggcgtaa ccaaaccctc attaggctgt gggttgctgg tctctcgtcg
     1621 ggcgagttgc tcggcccgtg ccctgagcac gagcaaggtg agcgttcctg caacgacgat
     1681 ggcggacagg ttgatcacca gctggaccgc ggagcctatc gcctcgctcg gtacccaata
     1741 ggcgagtgca accgccgcgt tggctgctgc ggggatcgtc gtcactgaga tgagaacgcc
     1801 caccagagcg ccggacttgg cggaggtaag cgcgagcatg cctgc
//
LOCUS       DATAOZ010000617         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_245674, whole genome shotgun sequence.
ACCESSION   DATAOZ010000617 DATAOZ010000000
VERSION     DATAOZ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245674"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VHR64_15430"
     CDS             <1..456
                     /locus_tag="VHR64_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3304277.1"
                     /translation="GAAGMHGWAPEVISAVGAPQTTPEGYLSVFFASSDTSREAGRQA
                     AGRIFGGRTTDRDERTTWQTRQAQYDAVCAWGIPNHALLQRVAAIELPVLVANGDRDP
                     MVLPRYSYLLAGLLPDARLTIYPDSAHGFLFQYHEKFAAEVHAFLDESN"
     gene            771..1622
                     /locus_tag="VHR64_15435"
     CDS             771..1622
                     /locus_tag="VHR64_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX3304278.1"
                     /translation="MSSEAVWAVPNYFRVCDGVRVRFADNRADSDITVLLLAPWPETL
                     WAFRRIWDRVSTVARVVAIDMPGFGHSDGRPELIAPDSSGAFLARLIDEWALGSPHLV
                     GPDVGTAAALFLAARTPERVTSLTIGGGAVRFPIEAGGALKDIIEAPSLDIVRRLDAR
                     VNIGRSVDAVAGSDSEPDVHEDYVSAYDLGRFAESARFVRHYPEQNPVLRDLLSTITT
                     PTQILAGRHDDLVPWSNNQYLHDLLPNSEIHGLDAGHFAWEQAPEEYGGLVAEWVSGR
                     YRRVGAS"
     gene            complement(2025..2513)
                     /locus_tag="VHR64_15440"
     CDS             complement(2025..2513)
                     /locus_tag="VHR64_15440"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX3304279.1"
                     /translation="MTSTDQSNEPARQTKSDLGVDMRLEVVTLPVSDVDRAKSFYQGL
                     GWRLDADFVFGDDIRGVQLTPPHSACSISFGKGLTTAEPGSAQRMELVVSNIDAARED
                     LISRGVEVSDVFHRDGARLLPGPDPERRSYLTYASFSDPDGNSWLLQEVTTRLPGREW
                     DD"
BASE COUNT          471 a          875 c          873 g          476 t
ORIGIN      
        1 ggtgctgcag ggatgcacgg ttgggccccg gaggtgatta gcgcggtcgg tgctccgcag
       61 acgaccccgg agggatacct ctccgtcttt ttcgcctcca gcgacacgag tcgcgaggcg
      121 ggtcggcagg ccgctggccg catcttcgga ggcaggacaa ccgaccgcga cgagcggacg
      181 acgtggcaga ctcgccaggc gcaatacgac gctgtctgcg cctggggcat ccccaaccat
      241 gcgctgcttc agcgggtcgc cgcgatcgag cttccggtgc tcgtcgccaa cggcgaccgc
      301 gaccccatgg tcctcccgcg ctattcgtac ctgctcgccg ggttactgcc ggacgcgcgg
      361 ctcacgatct acccggactc ggcccacggc ttcctcttcc agtaccacga gaagttcgct
      421 gccgaggtgc acgcgttcct cgatgagtcc aactgattcg gatggttgcc ggtgacgccg
      481 ccgccgcggg cttcgcgcgc cccatcggct tgctctaggc atggcggcgg ccttgatgcg
      541 ctgggaggag gtgcccgacc tgtcgctcgc catcaccccg cgatcatcca cgaatggacg
      601 cccgagctcc aacgtcgggc gagcgagtga tatctgcggg tcccggagcg gcgtagacgc
      661 agaccggcag ggacagtggc tgtagcagat gtccctgggg catgcacacc aatgggcgga
      721 tcatcgatgg atgacaccgc cagaaggcag taaaagagga ggctcatcag atgagcagcg
      781 aggcggtttg ggcggttcct aattacttcc gagtctgcga cggggtgcgg gtgaggttcg
      841 ccgacaacag ggcagactcg gatatcacgg ttctgctgct ggcgccgtgg cccgaaaccc
      901 tgtgggcctt ccgtcgcatc tgggaccgcg tctcaacagt cgcgcgcgta gttgccatcg
      961 acatgccggg attcggccat tccgatggcc gccccgaact gatcgcgccg gactcttcgg
     1021 gagcattcct ggcccgtctg atcgacgaat gggccctcgg gtctccgcac ctcgtcggcc
     1081 cagacgtagg cacggccgcg gcgctcttcc tcgcggcaag aacgccggag cgcgtcacga
     1141 gcttgacgat cgggggcggc gcggtccgct tcccgatcga agcgggcggc gcactcaagg
     1201 acatcatcga agcgccgagt cttgacatcg tgcgcaggct ggatgccaga gtcaacatcg
     1261 gccggtcggt cgatgcggtc gccggaagtg acagcgaacc cgacgtccac gaggactatg
     1321 tcagcgccta cgaccttggg cgcttcgcgg agtcggcccg attcgttagg cattaccccg
     1381 aacagaaccc ggtgttgcgt gacctgctct cgacgatcac cacgccgacc cagatcctgg
     1441 ccggccgcca tgatgacctg gtgccgtggt ctaacaacca atacctccac gacctcctcc
     1501 ctaacagcga gattcatggt ctcgacgccg gtcatttcgc atgggagcaa gcgcccgagg
     1561 agtacggcgg cctggtcgcc gagtgggtca gtggccgtta tcgacgcgtc ggagcgagct
     1621 agctgcgaga tctcgtcgag cgctgcgtgc gcacaccgcc gaggcggccg acgggcaagc
     1681 ggcttttctg gccacgacct gcctactgga gcgtcaacaa tttgcggccg gtcggctgat
     1741 gcgcatgctg ccgatctcgg gctgaaggtg gaccgattca tcgagtatct gcgccgccat
     1801 tcgtcgattg gatggccgaa gatgtcgact ccaacggagc tgcgcgcact ccgacgctct
     1861 tcatcaacga ccgccgccac cacgggccta cgacatcgaa gtcagggagc ctctcgaatg
     1921 cctgccgcct ccgtcaaccg ggtgaacgcc tagagccagc agacagccca gtctgacgga
     1981 atggcatcgg cgtggaaatc gtggcaggca aaggagaaac gacgtcagtc gtcccactcc
     2041 cggcctggta gccgcgtggt gacttcctgt aggagccagc tgttgccgtc cggatcgctg
     2101 aaagaggcgt atgtgaggta ggagcggcgt tccggatccg ggcctggaag aagacgcgcc
     2161 ccgtcgcggt ggaatacgtc actgacctcg acgccgcggc tgatcagatc ctcgcgcgcc
     2221 gcgtcgatgt tggagacgac tagttccata cgctgagcgg agccgggttc tgccgtggtg
     2281 aggcccttcc cgaatgagat cgagcacgcc gagtgagggg gcgtcaactg gaccccgcgg
     2341 atgtcgtcgc cgaagacgaa atcggcgtct agccgccatc ccaagccctg atagaagctc
     2401 ttggcccgat cgacatcgga gacgggcaac gtcacgacct cgagcctcat gtccacgccc
     2461 agatcgctct tcgtctgcct cgccggttca ttactttgat ccgtgctggt catatgacct
     2521 ccattgattg ctgtggcgag caaagtagcg cgcgccggcg tcgatcgtgg tgaagctgct
     2581 ccgttcgtcc aggtgtggcc gaagtccatg gtgccgaggc agatccggct gaccgtcaga
     2641 cctgagctgc ccaagtgcgc gtacgtcatt tcctcgctcg acgacacgcc cacgc
//
LOCUS       DATAOZ010000618         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4976535, whole genome shotgun sequence.
ACCESSION   DATAOZ010000618 DATAOZ010000000
VERSION     DATAOZ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4976535"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1133
                     /locus_tag="VHR64_15445"
     CDS             78..1133
                     /locus_tag="VHR64_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304280.1"
                     /translation="MIESSWRSALAGIAAGAAILIRPNLAPIAGVVALAVFCAGRVPP
                     RAVVRTWLAFAGGVAPFVILVALLQNAMYGSPFRSGYGSLGALFKLEHIWPNLQRYPL
                     WLLQTETPIVLLAFLAPWLMRDPPARRRAFWLLAFAGAVFACYIPYEVFDAWWYLRFV
                     LPAYPPLLVLTAAAVMALFARAGEAWRSAGVVAVALLSVFLVRGAVERHAFGLWEFER
                     RFRLAGEYVAARLPQNALIVTAQESGSVRFYSGRPTLTWRALPPDGLDRALAFVRAHG
                     YRPYFLIETGEQAEFVQQFEGKSPLGNLGWPPGVDINHMLRIYDPDDYANYRAGVPIR
                     TDRIWITKKRNWLGFRF"
     gene            complement(1218..2486)
                     /locus_tag="VHR64_15450"
     CDS             complement(1218..2486)
                     /locus_tag="VHR64_15450"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX3304281.1"
                     /translation="MQAHRYLAFVALVLAAVVTAQPIDAQSSLKSRVYATGLSSPVAF
                     VQDPTRSSRQFVVEQGGRIRVIESGAVQSTDFLNLSSVIASGGERGLLGMAMAPDYAS
                     SGRFYVNFTDPAGNTVIARFLRSGNPAVADPSSRFDLVWPSGLKYIFQPYSNHNAGNL
                     AFGPDGFLYIALGDGGSGNDPENRAQDPAQLLGKCLRIDVNVALNHATGYVVPSTNPF
                     VSGGPAGVLKEIWSFGLRNPWRYSFDMPSLGGTGALIIADVGQGAWEEVDYEPAGRSG
                     RNYGWRVREGAHPNVTSPPPVYVPATGLTEPVWEYSHSVGQSITGGFVYRGSALPSFR
                     GRYFFADYVQGRVWSLALTINPSTGEATVSDLVDHTASLGGSSVIGNISSFGLDAAGE
                     LYVVNHTGGAIVQIVPAVPPTPPTNLRIIR"
BASE COUNT          494 a          856 c          871 g          495 t
ORIGIN      
        1 aggtcgtgca gccaatgagc gacgttcccg caacggcgat gtgggcagcg tcgctggtca
       61 cggtcactca cccgcggatg atcgaatcga gctggcgaag cgcgctcgcc ggcatcgccg
      121 cgggcgcggc cattctgata agacccaatc tggcgccgat cgccggcgtg gtcgcactcg
      181 cggtcttctg cgccgggcgc gtaccgccgc gcgccgttgt ccgaacgtgg ctcgcgtttg
      241 cgggcggcgt ggcgccgttc gtgatcctgg tcgcgctgct gcagaacgca atgtacgggt
      301 cgcccttcag atcgggctat ggcagcctcg gggcgctctt caagctcgaa cacatttggc
      361 cgaatctgca gcgctatccg ctgtggcttc tccagaccga aacaccgatc gtgctgctcg
      421 cgtttctcgc tccgtggctg atgcgcgatc cgcccgcgcg gcgccgcgca ttctggctgc
      481 tggcgttcgc tggcgcggtc ttcgcctgct acattccgta tgaagtcttc gacgcatggt
      541 ggtacctgcg cttcgtgctg ccggcttacc ctccgcttct cgtgctgacc gcggcggcag
      601 tgatggcgct tttcgcgcgg gccggtgagg cctggcggtc ggccggggtg gtggccgtcg
      661 cgctgctgtc ggtctttctc gtccgcggag cagtcgaacg ccacgcattc gggctgtggg
      721 aattcgaacg gcggtttcgc ctcgctggcg agtacgtcgc cgcgcgtctc cctcagaacg
      781 cgttgatcgt gaccgcccag gagagcggca gcgttcgctt ctattcaggc cgaccgacgc
      841 tgacctggcg cgcgttgccg cccgacggcc tcgatcgggc gctggccttc gtccgcgcgc
      901 acggctatcg cccctacttc ctcattgaaa caggagaaca agcggaattc gttcaacagt
      961 tcgaaggcaa gagtccgctc ggcaaccttg gctggccgcc cggcgtcgac atcaaccaca
     1021 tgctgcggat ctacgacccc gacgactacg cgaactacag agccggcgtc ccaatcagga
     1081 cggatcggat ctggatcacg aagaagagaa actggctcgg tttcaggttt tagtgtcccg
     1141 agcgtatctc acaaaccgta tgttcgttgc caaggtgcga gtgagcatgg cgagcgagcc
     1201 acgcgatcgt ccctcgacta tctaatgatc cggagattcg tgggcggggt cggcgggacc
     1261 gcaggaacga tctgcacgat cgctcctccc gtgtgattga cgacgtacag ctcccctgcg
     1321 gcgtcaagtc cgaaagagct gatgttcccg atcaccgaac tgccgccaag gctcgctgtg
     1381 tgatcgacga gatccgagac ggttgcctcg ccggtggacg gattgatggt aagagccagc
     1441 gaccacaccc ggccctggac gtagtcggca aagaagtaac gcccgcggaa cgacggcagc
     1501 gcgctacccc gatacacaaa gccgcccgta atcgactgcc cgacggagtg gctgtactcc
     1561 cagaccggct ccgtcaatcc ggtagccggg acatacaccg gtggagggga cgtgacattc
     1621 gggtgggcgc cttcacgcac ccgccaaccg tagttgcggc cgctgcggcc ggccggctcg
     1681 tagtcaacct cttcccaggc gccctggccg acatcggcga tgatcagagc gccggtacct
     1741 cccagcgacg gcatgtcgaa cgagtaacgc cagggattgc ggagaccaaa gctccagatt
     1801 tccttcaaca cgccggcggg gccgccgctg acgaacggat tggtcgaggg gactacgtac
     1861 ccggtcgcgt gattgagcgc gacgttcacg tcgatgcgta ggcacttgcc cagcagctgc
     1921 gccggatcct gcgcgcggtt ttcgggatcg ttgcctgacc cgccatctcc cagcgcgatg
     1981 tagagaaagc catccggacc aaacgcgaga ttgccggcgt tgtgattgga gtacggttgg
     2041 aaaatgtact tcagccctga aggccaaacc agatcgaagc gcgatgacgg gtcagctact
     2101 gccggattgc cagaccgaag gaatcgcgcg atgacggtgt tccctgcagg gtcggtaaag
     2161 ttgacataga accggccgct agaggcgtag tcgggagcca tcgccatccc cagcagtccg
     2221 cgttcgccgc cagaggcaat gacagaactc aagttgagaa agtcggtcga ttggacagcg
     2281 ccgctttcaa tcacccggat ccggccaccc tgctcgacga cgaattgtct cgaactccgg
     2341 gttggatcct ggacgaatgc gacgggggag ctgagaccgg tcgcatacac ccggctcttg
     2401 agggatgact gcgcgtcgat cggttgtgcc gtgacgacgg ctgccaagac aagggccacg
     2461 aacgccaaat atcggtgggc ttgcatggtg gcggccgcca atgcacgaat cgagccgaag
     2521 gatcccaata gccgcagtca gactatcatt gctccaatgc gccgttcttg gattgggatt
     2581 acggtttttg cggccgcctg cggctcgagc acgcctgcac cgccttctac ggggcccggg
     2641 aacggcacga cggagaccat caccggacgt gaacgaatcg gatgggatca accggcagcg
     2701 gacagtgcag aactgg
//
LOCUS       DATAOZ010000619         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2609944, whole genome shotgun sequence.
ACCESSION   DATAOZ010000619 DATAOZ010000000
VERSION     DATAOZ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609944"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1656
                     /locus_tag="VHR64_15455"
     CDS             <1..1656
                     /locus_tag="VHR64_15455"
                     /inference="COORDINATES: protein motif:HMM:NF036501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304282.1"
                     /translation="DEMSNEEYQALIRIRNGAELRTRLTDVADAQYRRDLIATVKDTP
                     DEEMQALVSNLGGHDLPALLDVLNKADQVTDAPSVIFAYTIKGYGLPMAGDPLNHSLL
                     LTQDQMDQVQATYGIDPDDPWAPLDPASEAGKLCTAATERLATSTLRDDGLDIAFPDS
                     LDTRNPQTTSTQEALGRSLTRLADFEEARSRIVTLSPDVATSTHLSGWINKVGVFSQE
                     QSHDYEDPGQKMLKWLPSPSGQHIELGISEMNLFMALSQFGLSDELTGQHLIPIGTVY
                     DPFVCRGLDALIYGLYVGAKMIFAGTPAGVSLSPEGGAHQSTVTPSLGIELPGVRFYE
                     PAFAHEVEWMLLDAIARCADRENGSSTYLRLSTKPVDQSLAKQITGRVEPAELRESVL
                     AGGYRLIEWQTDAADLDPRDCVQIAVAGIMVPEAVEAARRLHAEGVAANVLVITSAER
                     LFADVRTARRTQLRNAGAPLDLGHLESLIPAEERRAPIVTVQDGASHSMAFLGSVWGV
                     PVVPLGVDEFGQSGSRADLYRATGIDADQIFNAAMLSLDLITT"
BASE COUNT          296 a          579 c          547 g          284 t
ORIGIN      
        1 gacgagatga gcaacgagga gtaccaggcg ctgatccgga tcaggaacgg cgccgagttg
       61 cggacccggt tgaccgacgt cgccgacgcg caataccgcc gcgacctgat cgccaccgtc
      121 aaggacacgc cggacgagga gatgcaggca ctggtcagca acctcggcgg gcacgacctg
      181 ccggccctgc tcgacgtcct gaacaaggcg gaccaggtga ccgacgcgcc gtcggtgatc
      241 ttcgcgtaca cgatcaaggg ctacggtctg ccaatggccg gtgacccgct caaccattcg
      301 ctgctgctta cgcaggatca gatggaccag gtgcaggcga cgtacgggat cgacccggac
      361 gacccatggg ccccgctcga tcctgcatct gaggcgggca agctctgcac ggccgcgact
      421 gaacggcttg ctaccagcac cctccgcgac gacgggctcg atatcgcgtt tccggattcg
      481 ctcgacacgc gcaatcccca gacgacatcg acccaggaag ccctcggccg ctccctgacc
      541 cggctggccg atttcgagga ggcgcggtcg cgcatcgtca cgctctcgcc ggatgtggca
      601 acgtcgacgc atctctccgg ctggatcaac aaggtcggcg tcttctcgca agagcagtct
      661 cacgactacg aggatccggg ccagaagatg ctcaagtggc tgccgagccc gagcgggcag
      721 cacatcgagc tcggcatttc ggaaatgaac ctgttcatgg cgctcagcca gttcgggctc
      781 tcggacgaat tgaccggcca gcacctgatt ccgatcggta cggtctacga cccgtttgtc
      841 tgccgtgggc tcgacgcgct gatctacggg ctctacgtcg gcgcgaagat gatctttgcc
      901 ggtacgccgg ccggcgtttc gctcagcccg gagggcggcg cgcaccagag caccgtcacg
      961 ccgtcgctcg gaatcgagct gccgggtgtc cgattctacg agccggcctt cgcgcatgag
     1021 gtcgaatgga tgctgctgga tgcgatcgcc cgctgtgccg atcgtgaaaa cggctcatcg
     1081 acctatctgc ggctgtccac gaaaccggtc gaccagtcgc tggcgaagca gatcaccggc
     1141 cgcgtcgaac cggctgagct ccgcgaatcg gtcctcgccg ggggatatcg cctgatcgag
     1201 tggcagaccg acgcggccga tctcgatccg cgcgattgcg tgcagatcgc cgtcgccggg
     1261 atcatggtgc cggaggcggt cgaggcagct cgccggctcc acgccgaggg cgtcgcggcc
     1321 aacgtgctgg tcatcaccag cgccgagcgg ctcttcgcgg acgtccgcac tgcccgccgg
     1381 acgcagctgc gcaatgccgg agcgccactt gatctcggtc atctcgaatc gctgatcccg
     1441 gccgaggaac ggcgcgcgcc gatcgtcacg gttcaggacg gcgcatcgca ctcgatggcg
     1501 ttcctcggct cggtctgggg cgtcccggtc gtgccgctcg gcgtcgacga gttcggccag
     1561 tctggatcgc gcgcggatct ctaccgcgcg accgggatcg acgccgacca gatcttcaac
     1621 gcggcaatgc tctcactcga cctgatcacc acttagtctg gttgactcga ttgcacaacg
     1681 tagcgcaggg ttaccacgcc ccgctt
//
LOCUS       DATAOZ010000620         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6029353, whole genome shotgun sequence.
ACCESSION   DATAOZ010000620 DATAOZ010000000
VERSION     DATAOZ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6029353"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /gene="pgk"
                     /locus_tag="VHR64_15460"
     CDS             <1..493
                     /gene="pgk"
                     /locus_tag="VHR64_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX3304283.1"
                     /translation="NEIGTSLAEPDALDDARRVVRLAGQYGTAVLLPSDVVIAPSIDS
                     DQTSTISADAVPSDQSIFDIGPDTIAGFSKRIGAAKTIFWNGPMGVFEKPQFATGTKE
                     IARAVAESRAYSVIGGGDSVAAVEEAGVAGQISHISTGGGASLEFVEGRALPGIEALR
                     GER"
     gene            490..1233
                     /gene="tpiA"
                     /locus_tag="VHR64_15465"
     CDS             490..1233
                     /gene="tpiA"
                     /locus_tag="VHR64_15465"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX3304284.1"
                     /translation="MTQRRLVLGNWKMNTTLEEAVEIADHLRDEPIGEGVTVGIAPPF
                     PWLQPVKQALGKSGVLLGAQTFAAASNGAFTGEVSAAMVAELCDFALIGHSERRALHG
                     ETDDVVREKLLRALEVGLMPVVCVGETLAERMAGDQERVVLRQIHAVLEGIENEELHR
                     LVIAYEPVWAIGTGQTASPQDASAMCGLISVALTGRKLMGIPVLYGGSVTAANAASLI
                     AEENISGFLVGGASLKPDDFLTIVRAAQV"
     gene            1299..1523
                     /gene="secG"
                     /locus_tag="VHR64_15470"
     CDS             1299..1523
                     /gene="secG"
                     /locus_tag="VHR64_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX3304285.1"
                     /translation="MDTALNLVMIIISAVLILVVLLQTKGSSFSGAFGGDTGSINRTR
                     RGLEKTLFQFTIIIAALFGIVAFLSSIITG"
     gene            complement(1520..1744)
                     /locus_tag="VHR64_15475"
     CDS             complement(1520..1744)
                     /locus_tag="VHR64_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304286.1"
                     /translation="MGFIWLFTASVLIAAGTGLGVLGVGGHLLVRFRPAASSNISYDP
                     STIASFAPIPLGLGCLLIVAFVRRSRRLGN"
     gene            1842..3068
                     /gene="coaBC"
                     /locus_tag="VHR64_15480"
     CDS             1842..3068
                     /gene="coaBC"
                     /locus_tag="VHR64_15480"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX3304287.1"
                     /translation="MITDPHLLPYQPLLGKLVLLGVTGGIAAYKAADLASNLVQLGAD
                     VHVLMTHSATEFVGPTTFAALTQNPVHDTAIERWEAGYTGHISLGQSAAAFVVAPATA
                     NTIAKLALGLADDMIGTVALTTQAPLVIAPAMEHEMFHHPATQAHVATLRDRGALIVG
                     PDTGRLASGEYGEGRLTTAAIITGAVRQAIGLGGPLTGKTIVVTAGGTFEALDPVRYI
                     GNRSSGRMGYSLAQSAIDAGARVILITGPSNLPTPYGACVVRVESAEQMNTAVQTHVK
                     AADAILMTAAVADFRPSQPSLEKIKKQEGQTGLTVELDRNPDIIGGLRDAPLLRVGFA
                     AETSNHVLNATDKLTRKNLDMVIVNDAVDTIGSTTIQATLVSRSNEPEILPRMVKERM
                     AATIIERLPGLLENHS"
     gene            3065..3400
                     /locus_tag="VHR64_15485"
     CDS             3065..3400
                     /locus_tag="VHR64_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304288.1"
                     /translation="MSSSSSRLPSLAPPSDGSQTSNLPVLLMLNCYAAAGAVFMSRAI
                     LKALEISDRYWVGRQIFRVTDPFARILELFPGASRTFVGSLSLADVTLVVGVILLPLG
                     ILAFGGRRR"
BASE COUNT          675 a         1112 c         1137 g          661 t
ORIGIN      
        1 gaacgaaatc gggacctcgc tggctgagcc ggatgcgctg gatgacgcgc gccgggtcgt
       61 tcggctcgcc ggtcagtacg ggaccgccgt gctactcccg tccgacgtcg tcatcgcccc
      121 atcgatcgac tcggaccaga cgtcaaccat cagtgccgac gccgtgcctt ccgatcagtc
      181 gatcttcgat attggcccgg ataccattgc gggtttttcg aagcggatcg gcgcagcgaa
      241 gacgatcttc tggaacgggc cgatgggcgt cttcgagaag ccgcagtttg cgacgggaac
      301 aaaggagatc gcccgggcag tcgccgagtc gcgcgcctac tctgtcatcg gcggcggcga
      361 ctcggtcgcg gcagtcgagg aggcaggcgt agccggtcag atatcgcata tctcgaccgg
      421 cggcggcgcg tcgctcgagt tcgttgaggg ccgtgccctg cccggcatcg aggcactgcg
      481 aggagaacga tgacccagcg acgcctagtc ctcggcaact ggaagatgaa cacgacgctc
      541 gaggaggcag tcgagatcgc tgaccacctc cgggacgagc caatcggcga gggcgtaacc
      601 gtagggatcg cgccgccgtt tccatggctc caaccggtca agcaggcgct cggcaagtcc
      661 ggcgtgttgc ttggtgccca gacgtttgcc gcggcttcga acggtgcttt caccggcgag
      721 gtctcggcgg cgatggtggc cgagttgtgc gatttcgcgc tgatcggcca ctccgagcgg
      781 cgagcgttgc acggcgagac cgacgatgtg gtccgcgaga agctgttgcg agccctcgag
      841 gtcggcctga tgcccgtggt ctgcgttggt gaaacattgg ccgagcgcat ggctggcgac
      901 caggagcggg tcgtcctgcg ccagattcac gccgtgctcg aagggatcga aaatgaggaa
      961 ctccaccgcc tggttattgc atatgagcca gtctgggcga ttggcaccgg tcagaccgcc
     1021 tcgccgcagg acgcgtcggc tatgtgtggc ctgatcagcg ttgcgctgac cggtcgtaaa
     1081 ctgatgggga tcccggtgct ttacggtggc agcgtcaccg cggccaatgc cgcgtcactg
     1141 atcgccgagg agaacatcag cggcttcctg gtcggcggcg ccagtctgaa gccggacgac
     1201 ttcctgacga tcgtccgggc ggcccaggtt tgaagatcgc gggggccaat ggtaggctgt
     1261 gttggccccg aattcacggt gcccaaagga gcgtctgagt ggatacagca ctcaatctcg
     1321 tcatgatcat catttccgcg gtcttgattc tggtcgtctt gcttcagacg aagggctcga
     1381 gcttcagcgg cgcattcggc ggcgacaccg gttcgatcaa ccgcacccgc cgcggcctcg
     1441 agaagacgct gttccagttc acgatcatca ttgccgcgct cttcggcatc gtcgccttct
     1501 tgagcagcat cattaccggc tagttcccca gcctgcgact ccgccgcacg aacgcaacga
     1561 tcaacaggca cccgagtccg agtggaatcg gtgcgaatga cgcgatcgtg ctcgggtcgt
     1621 agctgatgtt gctggacgcg gccggccgaa agcgcacgag cagatgcccg ccgactccga
     1681 gcacgccgag cccggtccct gccgcgatca acaccgacgc ggtgaagagc cagataaacc
     1741 ccaacacgcg cagccatata gaatgcgaaa tgcgtcgcat ggatcgacgt gaaaatactg
     1801 ggctgcccat cgcacgaagc atagcgggtt gacgaggaac aatgatcact gatccacatt
     1861 tgctgccgta tcaaccgctc ctgggcaagc tggtgctgct cggggtgacc ggcgggatcg
     1921 cggcgtacaa ggcagcggat ctggccagca acctcgtgca gctcggcgcc gacgtccatg
     1981 tgctgatgac gcactcagcg acggagttcg tcggtccgac gacgtttgcg gcccttaccc
     2041 agaacccggt ccacgacacc gccatcgagc gatgggaagc cggatacaca gggcacattt
     2101 cgctcggcca atctgccgcg gccttcgtcg tcgcgccggc caccgccaat accatcgcaa
     2161 agctcgcact tggcttggcc gacgacatga tcggcaccgt tgccctgacg acccaggcac
     2221 cgcttgtgat cgcgccggcc atggagcacg agatgttcca tcatccggcg acccaggccc
     2281 atgtggcgac gctgcgcgat cgcggcgcgc tgattgtggg acctgatacc ggtcggctcg
     2341 cctcgggcga gtacggtgag gggcgattga ctacggcagc gatcatcacc ggcgccgtcc
     2401 gccaggcgat cggactcggc gggccactga ccggcaaaac gatcgtcgtc acggctggcg
     2461 ggacgtttga agcgctggac ccggttcgct acatcggcaa ccgctcgagc ggccggatgg
     2521 gctactcgct ggcacagtcc gcaatagacg ccggtgcacg ggtcatcctg atcactggac
     2581 ccagcaactt gccgactccg tacggcgcct gcgtagtgcg ggttgaaagt gcagagcaaa
     2641 tgaataccgc ggtccaaacg cacgtcaagg ccgccgatgc gattctgatg acggcggctg
     2701 tggccgattt ccgaccctct cagccgagtc tcgaaaagat caagaagcag gaggggcaga
     2761 ctggcttgac cgttgagctg gaccgcaatc cggacatcat cggcggcctg cgcgatgctc
     2821 cacttttacg cgtcggattc gccgcggaga caagcaacca tgtcctgaac gcgactgaca
     2881 agttgacccg caagaacctg gatatggtca tcgtcaatga cgccgtcgat acgattggat
     2941 cgacaacgat tcaggcaaca ctggtttcgc ggtccaacga accagaaatc ttgccgcgga
     3001 tggtcaagga gcgaatggcg gcaacgatca tcgagcggct gcccggcctg ttggagaatc
     3061 actcatgagc agcagctcgt ctcgtctgcc gtcgctggct cctccatcgg acggcagcca
     3121 gacatcgaac ttgcctgtcc tgctcatgct caattgctat gccgcggcag gcgccgtatt
     3181 catgagccgc gcgatcctga aggcgctcga aatctcggac cgatactggg tcgggcggca
     3241 gatcttccgc gtgaccgatc cgttcgcccg catcctcgag ctctttccgg gcgcatcgcg
     3301 gacatttgtc ggctcgctct cgctcgccga tgtcacgctc gtcgtaggcg tcatcctgct
     3361 tccgcttgga atacttgcgt tcggtggtcg ccgccgataa gactgattcg cgacgctaca
     3421 atcacattgg atcgatggtc agtccgcggt ggactgcacg actttagggg catggatcgc
     3481 atatgactcg caacctggtg cagggaatta tcggattggt catggcagcg attgccactt
     3541 gggctacgaa caagctggtc gagcaactct tcgggccgga tactg
//
LOCUS       DATAOZ010000621         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4185087, whole genome shotgun sequence.
ACCESSION   DATAOZ010000621 DATAOZ010000000
VERSION     DATAOZ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4185087"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VHR64_15490"
     CDS             complement(<1..1254)
                     /locus_tag="VHR64_15490"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX3304289.1"
                     /translation="MPHEVDPTFLTLPVRSLADAALQRAQDLGAAHADFRLERIRGQS
                     VRLRDARVEGVSDDEDSGFAVRVVHEGTWGFAAAADLTAEAAAKAAELAIQVAKISSA
                     INSEPVELAEEPVYADVTWVSPYDVDPFDVPDPEKIGLLADRSARLLAHSAVDHVDAS
                     VYAVKEHKFYADLAGTITTQQRVRIHPEMTATAVDKESGRFETMRTLAHPAGRGWEWV
                     VGSTTEAGQLEELWGWDGELEQIPELLTEKMAAPSVEPGRYDLVIDPTNLWLTIHESI
                     GHATELDRALGYEAAYAGTSFATFDQLGNLRYGSELMDVIGDRTVDHGLSTIGYDDEG
                     VATQQWDIVRDGVLVGYQLDRRMARLKRLGRSNGCAFADSPSHVPIQRMANVSLQPDP
                     AGPSTAELISGVERGIYILGDKSWSI"
     gene            1427..2131
                     /locus_tag="VHR64_15495"
     CDS             1427..2131
                     /locus_tag="VHR64_15495"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HEX3304290.1"
                     /translation="MTRSVLVTGGNRGIGLAIARAFSAAGDKVAITYRSGEPPEGLFG
                     VRCDVTDQAAVEAAFDSVESQQGPVEVLVANAGITRDKLLLRMAEDDFTVVLDTNLTG
                     SYRVAKRATRSMLRARRGRLIFISSVVGLLGSAGQANYAASKAGLVGLARSLARELGS
                     RNITANVVAPGFVSTDMTEALHEERRSEILAQVPLGRYATADEVAAVVRFLAGEDAAY
                     ITGAVIPVDGGLGMGH"
     gene            2174..>2705
                     /locus_tag="VHR64_15500"
     CDS             2174..>2705
                     /locus_tag="VHR64_15500"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX3304291.1"
                     /translation="MGILDGKRVLVTGVITDASIGFHIARVAQQEGARVVLTGYGRLS
                     LVERIAKRLPAEAPVVELDVTDADHLASLAERLRPYVDRLDGVVHSIGFAPKSALGGN
                     FLQTEWPDVSTALHVSAYSLKALATACAPLMKDGGSIVAMDFDATVAWPKYDWMGVAK
                     AALESCGRYLARDLGPS"
BASE COUNT          428 a          906 c          908 g          463 t
ORIGIN      
        1 gatggaccag gacttgtcgc cgaggatgta gatgccgcgc tcgacgccac tgatcaactc
       61 cgccgtcgat ggtccggccg ggtccggctg caacgagaca ttggccatcc gctggatcgg
      121 cacgtggctc ggcgagtcgg cgaacgcgca tccgttggag cggccgagtc gcttcaggcg
      181 cgccattcgc cggtcgagct gatagccgac gagcactcca tcgcgcacga tgtcccactg
      241 ctgagtcgcg acgccttcgt cgtcatagcc gatggtggac aggccatggt cgacggtccg
      301 gtcaccgatg acgtccatca gctccgagcc gtatcgcaaa ttcccgagct gatcgaaggt
      361 ggcgaacgat gtacccgcat acgctgcctc gtagccaagg gcgcgatcca gctcggtggc
      421 atgcccgatc gactcgtgga tcgtcagcca caaattcgtc ggatcgatca ccaggtcgta
      481 gcgccccggc tccacgctcg gcgccgccat cttctccgtc agcagctcag ggatctgctc
      541 gagctcgccg tcccaccccc acagctcttc gagctggccc gcctcggtcg tcgatccgac
      601 gacccactcc cagcctcgac cggccgggtg ggcgagggtg cgcatggtct cgaaccggcc
      661 ggactccttg tccacggcgg tcgccgtcat ctccggatgg attcgcaccc gctgttgcgt
      721 cgtgatcgtc ccggcaagat cggcgtagaa cttgtgctcc ttcaccgcat aaaccgacgc
      781 gtcgacgtgg tcgacggcgc tgtgcgcgag cagccgggcg ctgcggtccg cgagcaggcc
      841 gatcttttcc gggtcgggca cgtcgaacgg gtcgacgtca tagggcgaaa cccaggtgac
      901 gtcggcgtag acaggctcct cggccagctc gaccggctcc gagttgatcg cagagctgat
      961 cttggcgacc tggatggcaa gctcggcggc cttcgccgca gcctcggccg tcagatcggc
     1021 cgcagcggcg aatccccagg tgccttcgtg gacgacgcgc accgcgaacc cgctgtcctc
     1081 gtcgtcgctt accccttcga cacgcgcatc gcgcagccgc acgctttgcc ctcgaatccg
     1141 ctcgagccta aagtccgcat gggcggcgcc gagatcctga gcccgttgca gagccgcgtc
     1201 ggcaagcgat cggaccggca gagtgaggaa ggtcgggtcg acctcgtggg gcatctgcgc
     1261 ctccagcgct tgctgtgcat gtcgatcgga ccgaaccgat cctgtcacgc cgccgacagc
     1321 cgttgctgat cgccgtagag cgtcggggcc gaacagaccg cgagggctgg ctcggtgagc
     1381 ggggccgatt cgcggtaccg acaggtagcg ggtagcgtcc ctggccgtga ctcgatcagt
     1441 actcgtcacc ggcggtaacc gcggcatcgg cctggccatc gcccgggcgt tctccgctgc
     1501 gggggacaag gtcgcgatta cctaccgcag cggcgagccg ccagaaggac tcttcggcgt
     1561 ccgctgcgac gtcacggacc aggcggcggt tgaggcggcg ttcgattccg tcgaatcgca
     1621 gcagggaccg gtggaagttc tggtggcgaa cgccggcatc acaagagaca agctgctgct
     1681 gcggatggcc gaggatgact tcaccgtggt ccttgacacc aacctcaccg ggtcctaccg
     1741 cgtcgcgaag cgagccacga gatcgatgtt gcgtgccagg cgcggccggc tgatcttcat
     1801 ctcgtcggtc gtcggactgc tcggctcggc cggccaggcg aactacgcgg cctccaaagc
     1861 cgggctggtc gggctcgcca ggtcgttggc gcgcgagctc ggttctcgca acatcaccgc
     1921 caacgtcgtg gcacccggct tcgtcagtac cgacatgacc gaggcgttgc atgaggagcg
     1981 gcgctcggag atcctcgcgc aggtaccgct gggccggtat gccaccgccg acgaggtcgc
     2041 cgcggtggtc cggttccttg ccggagagga cgccgcctac atcactggag cggtgatccc
     2101 cgtcgacggc ggtctcggta tgggtcactg accaaggtcg gggcaccgac cagatcggag
     2161 aggtagggca agcgtgggca tcctcgatgg caagcgggtc ctggtgaccg gggtcattac
     2221 cgacgcgtcg atcggcttcc atatcgcccg ggtcgcgcaa caggagggag cgcgggtcgt
     2281 cctcaccgga tacggccggc tcagcctcgt cgagcggatc gccaagcggt tgccggccga
     2341 ggctccggtc gtcgagctcg atgtcactga cgcggatcac ctcgctagcc tcgccgagcg
     2401 gctgcgaccg tatgtggatc ggctcgacgg cgttgtgcat tcgatcggct tcgcgccgaa
     2461 gagcgcattg ggcggcaact tcctgcagac cgagtggcct gacgtgtcga ccgcgctgca
     2521 cgtatcggcg tactccctca aggccctcgc caccgcgtgc gcgcccctga tgaaggacgg
     2581 cggctcgatt gtggccatgg acttcgatgc cacagtggct tggccgaaat acgactggat
     2641 gggcgtggca aaggcggctc tcgagtcctg tggccgctac ctggctcgcg atctcggacc
     2701 gtcag
//
LOCUS       DATAOZ010000622         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7077055, whole genome shotgun sequence.
ACCESSION   DATAOZ010000622 DATAOZ010000000
VERSION     DATAOZ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7077055"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..823
                     /locus_tag="VHR64_15505"
     CDS             272..823
                     /locus_tag="VHR64_15505"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX3304292.1"
                     /translation="MVRDLVVQAQNGDRDAFAALVHMTSDRMYALAARILRDNDLAED
                     ALQSALITVWRQLPTLRDPDRFEAWVRRLLVHACYAEARRRRSWSTHVRVLPVDGPAA
                     PDTLLSIDDRDALDRAFRRLSVEQRAVFVLHHHIGLPLTEIAETVGIPAGTARSRLHY
                     ATRVLRAAVKADAAPVAPEGRMA"
     gene            820..>1772
                     /locus_tag="VHR64_15510"
     CDS             820..>1772
                     /locus_tag="VHR64_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304293.1"
                     /translation="MTSERDFDRLARAWLDLGPDEAPDRVIAAVLQAADTTPQVRRRG
                     PRPFRRSNTMTRYVAAAGLAAALILAIGGGMLLTGSSKTPAVVAPSTVPTPSATTGAS
                     IPRELQARWMGGHKQPFGADAGSTILIDANAIALSQSNSNDQAILSADASSAGEGRMR
                     LEQADPSTILCHEGDAGDYSWSLSAGGRILTIQSVSDACAARPGLLTGTWWRMDCADK
                     NDNCLGNLEAGTYASQFITPRLAPGAAWQPVFGALSYTVPAGWANSADWPSSFTLLPS
                     RDYDGTTADTTAEIYHGIYVLTDPAISAQDLACSATDQAGVG"
BASE COUNT          293 a          616 c          583 g          280 t
ORIGIN      
        1 gacgacgacc gcgaccagga agacctgcga ctgggtcgtt ccagcgaaca ggatgaggac
       61 cacatacgtc gcgatggcgc cgagcccggc accgatgatc cgttgcatcc gatcctccgg
      121 cagggcggtc ggtcggggtg aaacgccgct ccggcgagcc tagggggcgt tgccgcacag
      181 cgtcaatccg cctaccgtgc tgaacgttcc ggactcgcaa accactcatt gggtgtgacg
      241 gcgtcgcttg gggccgtcga cagagggagt gatggtgcgg gatctcgtcg tccaggcaca
      301 gaacggcgat cgcgatgcgt tcgcggcgct ggtccacatg accagcgacc ggatgtacgc
      361 gctggccgct cggatcctgc gcgacaacga tcttgccgag gacgccctgc agagcgctct
      421 tatcaccgtc tggcgccagc tcccgacctt gcgtgacccg gatcgcttcg aagcctgggt
      481 caggcgcctg ctcgtccatg cgtgctacgc cgaggccagg cgtcggcgct cgtggtcgac
      541 gcacgttcgt gtgctgccgg tcgacgggcc agcagctccg gataccctgc tcagcatcga
      601 cgaccgcgat gcgctcgacc gggcgttccg acgcctgtcg gtcgagcaac gcgcggtctt
      661 cgtcctgcac caccacatcg ggttgccatt gaccgagatc gccgaaaccg tcggcatccc
      721 ggccgggacg gcccgttcgc gactccacta cgcgacgcgt gtgctgcgtg cggccgtcaa
      781 ggccgacgcc gcgcccgtcg cccccgaagg acggatggca tgacaagcga acgcgacttc
      841 gaccgtctcg cacgagcctg gctcgacctg ggacctgacg aagcgcccga tcgcgtcatc
      901 gctgccgtcc tccaggccgc ggacacgacg ccgcaggtgc ggcgccgggg accacggccc
      961 ttccggaggt caaacacgat gactcgctac gttgctgccg ccggactggc ggcagcgctg
     1021 atcctggcga tcgggggcgg gatgctcctg accggatcga gcaagacacc tgcagtggtc
     1081 gcgccatcga cggtgccgac cccctcggca acgacgggcg catcgatccc cagggagttg
     1141 caggcacgct ggatgggtgg ccacaagcag ccattcgggg cagacgcggg ctcgacgatc
     1201 ctcatcgatg cgaatgccat cgcgctgtca cagtccaact cgaacgatca ggccatcctc
     1261 agtgccgatg cctcctccgc gggagaaggc cggatgcggc tggagcaggc ggatcccagc
     1321 acgatcctct gccacgaagg cgatgccggt gactactcct ggtcgctctc ggcaggcggt
     1381 cggatcctga cgatccagtc ggtcagtgat gcctgcgccg cgcgtccggg tttgctgacc
     1441 ggaacctggt ggcgaatgga ctgcgccgac aagaacgaca actgcctcgg gaacctcgag
     1501 gccgggacat atgcatcgca gttcatcacg ccgcgactgg cacccggcgc tgcgtggcag
     1561 cccgtcttcg gtgccctcag ctacaccgtg ccggcgggct gggcgaactc cgcggattgg
     1621 ccgtcgtcat tcaccctgct gccctcccgc gactacgacg gcacgacggc cgacaccacc
     1681 gctgagatct atcacggcat ctacgtcttg accgaccctg ccatcagtgc ccaggatctg
     1741 gcctgctcgg cgaccgatca ggctggggtc gg
//
LOCUS       DATAOZ010000623         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2872080, whole genome shotgun sequence.
ACCESSION   DATAOZ010000623 DATAOZ010000000
VERSION     DATAOZ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2872080"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VHR64_15515"
     CDS             complement(<1..271)
                     /locus_tag="VHR64_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304294.1"
                     /translation="MFAIGMAGLGILSLIYRDFALNWQPVPTWVPWRIPLALLSGAML
                     AAGGIGMLFRKTSQGAAALLTINVLVWLVLLEIPRVVASPAVEAMW"
     gene            complement(535..921)
                     /locus_tag="VHR64_15520"
     CDS             complement(535..921)
                     /locus_tag="VHR64_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304295.1"
                     /translation="MAAGGIRWLRVLVGSLLAHATVIGGGALCSLLPNPTTALLYALV
                     PLCLVATFVFGFWAARRAEDLFVVHGLLVGTLTALASAILSWRTTLPTVYVVANCLKL
                     AGGAAGGWLAAFHDGRVRRFQTTTPK"
     gene            complement(971..1258)
                     /locus_tag="VHR64_15525"
     CDS             complement(971..1258)
                     /locus_tag="VHR64_15525"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX3304296.1"
                     /translation="MCRNIRTLFNFEPPATEEEIRAASLQFVRKLSGFNTPSKANQAA
                     FDRAIEEVAAAAGTLIASLVTTAEPRDREVEAARARAKAALRFAPYKDSQS"
     gene            complement(1316..>1675)
                     /locus_tag="VHR64_15530"
     CDS             complement(1316..>1675)
                     /locus_tag="VHR64_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304297.1"
                     /translation="SARNRPSTMADTGFKPDPKMFADMPKAMREMRGPPPGMDALEAG
                     QRVLKGMRDNDLYILTTPEFEAEFRARGEAIVASLPTDVKPTEARVMFGRMILGKTTY
                     ATERDRKACERSRTRKA"
BASE COUNT          315 a          547 c          530 g          283 t
ORIGIN      
        1 gccacatcgc ctcgaccgcg gggcttgcca ccacgcgcgg gatctcgagc agcaccagcc
       61 acaccaggac gttgatggtc agaagagcgg ccgctccctg ggacgtcttg cgaaacaaca
      121 tcccgattcc ccccgcagcc agcatggcgc cggacagcag cgcgagcgga atgcgccacg
      181 gcacccatgt tggaaccggc tgccagttga gggcgaaatc ccggtagatg aggctgagaa
      241 tacccagtcc cgccatgccg atggcgaaca acagcggacc tgccccgggg gatcgcatga
      301 tgggcgggga tgttaacgct accatccggc gctgtaaatc cggtaccgcc ggagcagatt
      361 ccgggagttt tgcaataccc cctaatgaat gcggccggtc tgccgggcga gagtgatgtc
      421 aaacggggcc gttgcgtaga ctgcgcggcg ttgagtgact gaatggcaaa gttgagtaaa
      481 ggctatggaa atcgttctgt tcgagattgt tgcaaccgct gccctgctgc tggctcactt
      541 cggcgtcgtg gtctggaatc gcctcacgcg gccgtcatga aaggccgcga gccacccgcc
      601 cgccgcgccc ccggccagtt tcaggcagtt cgccaccaca tagaccgtcg gtaacgtcgt
      661 tctccaactg aggatcgcgg acgccagcgc ggtcagggtg ccgaccagca ggccgtggac
      721 cacgaacagg tcctcagccc gacgcgcagc ccagaatccg aacacgaagg tcgcaaccag
      781 acacagcggc accagtgcgt agagaagggc cgtggttggg ttcggcaaca gggagcacaa
      841 ggcgccaccg ccaatcacgg tggcgtgagc caatagggag ccgacgagga cgcgcagcca
      901 acgaatccca ccggcagcca tcgaactctc ctggcggtcc gggactggat ggggccgcgc
      961 ggcgagctta tcagctctga ctatccttgt atggagcgaa tctcaacgcg gctttcgcac
     1021 gtgcgcgcgc agcctcgacc tcacggtcgc gcggctcggc ggtcgtgaca agagaagcaa
     1081 tcagggtccc cgcagccgcc gccacctctt cgatggcgcg atcgaacgcg gcctgattgg
     1141 ctttcgacgg agtgttgaag ccgctgagct tcctgacgaa ctgcagggag gcggcgcgga
     1201 tttcctcttc cgttgccggc ggctcgaagt tgaacagcgt tctgatgttg cgacacatgt
     1261 tggcaccctg cggcacttgc cggacgcagc ccgcgccgcg tccggcacgt actggttacg
     1321 ctttcctggt ccgggaccgc tcacaagcct tgcggtcacg ctccgtggcg taagtggtct
     1381 tgccgagaat catccggcca aacatcaccc gcgcctcagt gggcttcaca tccgtcggca
     1441 gcgaagcgac gatggcctcg ccccgagccc ggaattccgc ctcgaactcg ggcgtggtga
     1501 ggatgtacag gtcgttatcg cgcataccct tcaggactcg ctgccctgct tccaacgcat
     1561 ccatgccagg cggcggcccg cgcatctccc gcattgcctt gggcatgtcc gcgaacatct
     1621 tcggatccgg cttgaaaccg gtgtcggcca tcgtgctcgg acggttgcgc gcgga
//
LOCUS       DATAOZ010000624         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5239408, whole genome shotgun sequence.
ACCESSION   DATAOZ010000624 DATAOZ010000000
VERSION     DATAOZ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5239408"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..492)
                     /locus_tag="VHR64_15535"
     CDS             complement(259..492)
                     /locus_tag="VHR64_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304298.1"
                     /translation="MVEQFERDDPERSASYRAATLVAARHQIGLTAWREGRQWAVRAA
                     YHDGVREAVTVTGRTAADAAWHALDQILRGMGG"
     gene            complement(497..721)
                     /locus_tag="VHR64_15540"
     CDS             complement(497..721)
                     /locus_tag="VHR64_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304299.1"
                     /translation="MSSVDLPLIRRRDHKPVGTLTIIRTNHAAFIDEHGTRRPSPVVL
                     DHLLISVKDAKGEEVTYLAVPMYQLPPESA"
     gene            complement(915..990)
                     /locus_tag="VHR64_15545"
     tRNA            complement(915..990)
                     /locus_tag="VHR64_15545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(955..957),aa:Gly,seq:tcc)
     gene            complement(1030..1102)
                     /locus_tag="VHR64_15550"
     tRNA            complement(1030..1102)
                     /locus_tag="VHR64_15550"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1067..1069),aa:Pro,seq:tgg)
     gene            complement(1238..>3324)
                     /locus_tag="VHR64_15555"
     CDS             complement(1238..>3324)
                     /locus_tag="VHR64_15555"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX3304300.1"
                     /translation="TRRRVAQSVATGLLGANLLVLIGNLDTFFHVLKTWPDQINPNFD
                     WTWGPSRAISNAITEFPYFTGLYADLHAHGINVPISMVVLAICLSLAQDPNLTTITTL
                     RPRANRETFGLLVRLGMLGLGVGSVATTNSWDLAEYVLFTFVALFMTTLGIRPFLLRA
                     GLTVFATGVVGAIAAVLFSPFYAKYVALFTTVGETRDKTSVIQFADHLGGLLAIVGVG
                     GSALLLARLRGRISWFVIDPLLPLGIVAVVLIVSIATNLGDTDSNSLTVRLATIVACA
                     VLAIPSLLVISDRQLEWLDDLCHGAVIAALVAEAVLVVDGRGVLALAVAFFVLGATMW
                     LFLEDRGERLVGAMVACAAGVFGAIEIVYLEDNLSGGDAYRMNTIFKFYNQVWVLLAI
                     AGAVVLGKALAGSGLLGWITGSQELGIVAGPFRPSGDGHQPTVDDGTVAGELIEDAAA
                     DDEQAELGPGQELDVWDEDEWTREDEPVDLEEHALMRRRWSRAAVIVGIVAIAMSLFY
                     PMLATRPRLDQRFDGHPGPGTLNAYDWMQYGTIIGGNGDTIHFAGDHDAIYWFINNVT
                     GSPVIMEAAIGPYRGNGSRFSINTGLPAVIGWGNHETQQRYNDDIGPREQEVREFYGT
                     TDLNRKREILAKYDVQYVIVGDVERYTAFNGEYWADPAGIAAIEQMVGTDLEIAFQSN
                     GTTVYRVVSRGT"
BASE COUNT          616 a         1086 c         1077 g          545 t
ORIGIN      
        1 gcaatatcgt ggcacccacg caaaaagaag actcgctggc tggggcgagt cttctctcac
       61 ggcacgcacc tggttgggag cggtgccggg agggggcgtg tgagcgggga accgttctga
      121 cccacctcct atgatgaggc cgaagcgaga tcgacatcac tgacgtccag cctaccgagc
      181 gcgcccagcc ctgtcatgaa gttttcttca tgaccgctgc tcgcggcgat gattcggcgg
      241 cgtcgcgagg agcagcggct agccgcccat cccgcgcagg atctgatcga gcgcatgcca
      301 ggcagcatcg gccgccgtgc gaccagtcac ggtgaccgct tcccggacgc cgtcgtgata
      361 ggcggcgcgc acggcccact ggcgcccctc gcgccaggcg gtcaggccga tctggtgccg
      421 ggcggcgacc agggtggccg cgcggtagct ggcactgcgt tcgggatcat cccgctcgaa
      481 ttgctcgacc atgaggctat gccgactccg gcggcaattg atacatcggg acggctaggt
      541 aggtcacttc ctcgcccttg gcatccttca cgctgatcaa gagatgatcc agcacgaccg
      601 ggctcggccg gcgcgtgcca tgttcgtcga taaacgcggc gtggttggtg cgaatgatcg
      661 tcagcgtgcc gaccggctta tggtcacggc gccggatgag gggaaggtca acgctggaca
      721 tctcagccga cccgcctgcg tgcaattgtg gggcgctgag aggcgcgcaa acgctgaatc
      781 tgactctggc tgccttgggc cgttttgcgc cgctctcgcg gacccaagga cacaaaaagc
      841 acacattgcc acatgggccc tgccatcatc tctgcgggga atccccaaaa gtggcgtaac
      901 tatcggcatt tcattggagc gggagaccgg actcgaaccg gcgacattct gcttggaagg
      961 cagacactct accaactgag ttactcccgc aacggagtat cgcgaccgct caccctcggg
     1021 catcccgggt cggggtggag ggactcgaac ccccgacctc agcgtcccaa acgccgcgcg
     1081 ctaccaactg cgctacaccc cgagagcgcg aaccgcacat acgagtataa aggcgagtag
     1141 gcgagaaggc gagaggcagg tagatggttg ttggtggtta gtggttggac ccttgttcaa
     1201 tcaaccaacc accatccact atcaacctgc tcacgactca cgtcccacga cttacgaccc
     1261 gatacaccgt cgtcccgttg ctctggaagg cgatttcgag gtccgttcca accatttgct
     1321 cgatggcggc gataccggcc ggatcggccc agtattcgcc gttgaacgcc gtgtagcgct
     1381 cgacgtcgcc gacgatcacg tactggacgt cgtacttggc caggatctcg cgcttgcggt
     1441 tcaggtcggt cgtgccgtag aactcgcgga cctcttgctc ccgcgggccg atgtcgtcgt
     1501 tgtagcgctg ctgggtctcg tggttgcccc agccgatcac ggccggcagt ccagtgttga
     1561 tcgagaagcg cgacccgttg ccgcgatacg ggccgattgc cgcctccatg atcaccggcg
     1621 agcccgtgac gttgttgatg aaccagtaga tggcatcatg gtcgccggcg aagtggatcg
     1681 tgtcgccgtt gccgccgatg atcgtgccgt actgcatcca gtcgtaggcg ttcaacgtcc
     1741 ccggcccggg gtgtccatcg aagcgctgat cgaggcgcgg ccgggttgcc agcatcgggt
     1801 agaagagcga catcgcgatc gcgacgatgc cgacgatcac cgccgcgcgc gaccaccgcc
     1861 gccgcatcaa cgcatgctct tcgagatcga ccggctcgtc ttcgcgcgtc cattcgtcct
     1921 catcccagac gtcgagctcc tgcccgggtc cgagctcggc ctgctcgtcg tccgcggccg
     1981 catcctcgat cagctcgccg gcgaccgtgc catcgtcgac ggtcggctga tgtccatcac
     2041 cggagggacg gaacggtccc gccacgatgc cgagctcctg cgagccggtg atccagccga
     2101 gcaggccgga gccggccagc gccttgccga gaacgaccgc cccggcgatg gcgagcagga
     2161 cccaaacctg gttatagaac ttgaagatcg tattcatgcg gtaggcatcg ccgccggaga
     2221 ggttgtcctc gaggtagacg atctcgatcg cgccgaacac gcccgcggcg caggccacca
     2281 ttgccccgac caaccgctcg ccccgatcct ccaggaagag ccacatcgtc gcgcccagca
     2341 cgaagaaggc gaccgccagc gccaggacgc cgcgaccatc gacgaccagc accgcttccg
     2401 cgacgagcgc cgcaatcacc gcgccatggc agagatcgtc gagccactcc agttgccggt
     2461 ctgagatgac cagcaggctc gggatggcca gcacggcgca ggcgacgatc gtggcgagcc
     2521 ggaccgtcag cgagttgctg tcggtgtcgc cgaggttggt cgcgatcgac acgatcagga
     2581 cgaccgcgac gatgcccagc ggcaggagcg ggtcgattac gaaccacgag atgcggccgc
     2641 ggagcctggc cagcagcagc gcgctgccac cgacaccgac gatcgccagc agcccgccca
     2701 ggtgatcggc gaactggatc acgctggtct tgtcccgcgt ctcgccgaca gtggtgaaca
     2761 gggcgacgta cttggcgtag aagggcgaga agaggacggc cgcgatcgcg ccgacgactc
     2821 cggtcgcaaa tacggtcagg ccggctcgga gcaggaacgg ccggattccc agcgtcgtca
     2881 tgaacagcgc gacgaaggta aagaggacgt actcggccag gtcccacgag ttcgtggtcg
     2941 ccacggaccc gacgcccagg cccagcatcc cgagccgcac gagcaggccg aaggtctcgc
     3001 gattcgcgcg cggccgcagc gtcgtgatcg tagtcagatt cgggtcctgc gcgagcgaca
     3061 ggcagatcgc cagcacgacc atcgagatcg gcacattgat cccatgggcg tgcaggtcgg
     3121 catagaggcc ggtgaagtag gggaactcgg tgatggcgtt gctgatcgcc cggctcggac
     3181 cccaggtcca gtcgaagttg ggattgatct ggtccggcca tgtcttcagc acgtggaaga
     3241 aggtgtcgag gttgccgatc agtaccagca agttcgcgcc gaggagacct gtcgcgacac
     3301 tctgggcgac gcgccgccgg gtcg
//
LOCUS       DATAOZ010000625         5935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8401337, whole genome shotgun sequence.
ACCESSION   DATAOZ010000625 DATAOZ010000000
VERSION     DATAOZ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8401337"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VHR64_15560"
     CDS             <1..308
                     /locus_tag="VHR64_15560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3304301.1"
                     /translation="IEVLLLQSRATLYHTIETWLDYPESRPQIGWQFAATKHAVTNNA
                     VEITDLAMRIAGSAGQFRRYPLERYFRDVRSGLGNPPIDDIALTIVGKAALGIGRRK"
     gene            322..849
                     /locus_tag="VHR64_15565"
     CDS             322..849
                     /locus_tag="VHR64_15565"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX3304302.1"
                     /translation="MSTLTSYLERKTVALKAARASASPKQDERMQHLTAQCTAEGGSG
                     IRRVRIRDFQIITDAGPAMAGYDLGPRSPEVLLGALGSCISHTVLIQAAVEGIPIESL
                     VVDVSADIDSLAGNTDVRTPLKNIAFEIQVESSASPDVFTRINELLPEICPVLNVITC
                     PQTITSAVVHNGIKV"
     gene            858..1355
                     /locus_tag="VHR64_15570"
     CDS             858..1355
                     /locus_tag="VHR64_15570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain protein"
                     /protein_id="HEX3304303.1"
                     /translation="MPDDVINHFAGIRPGSPLSGLRAQRPETLQHAQGSFQALLEPDD
                     PGGVSQEEREAIALRVATLERFPDIAAFHRERLRELDADDEVVSAVEQFPDGGDLPAR
                     LAAILRHTDMLTTSSRSGSPEAIAALREAGLSAREIVTISQLIAFMSYEIRMIALLRA
                     MGGAQ"
     gene            1352..1942
                     /locus_tag="VHR64_15575"
     CDS             1352..1942
                     /locus_tag="VHR64_15575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX3304304.1"
                     /translation="MSPASRRATQFTIERLDWAPWIETVTEIGLSDEELAAVYEQAPN
                     AKGSPYFATLALDLPALVERTGLFNNTMRSPGGGSNAERELAATAVSRINGCVYCASV
                     HAQRYVQYAKRPEVMDRLLEEGVDAPLNDAREQAIVDFSAKLTRNPAGLTSDDIKPLR
                     VAGLTDLEIYDIAQSAAMFAWANRLMQTLGDAVYPQ"
     gene            2185..2385
                     /gene="infA"
                     /locus_tag="VHR64_15580"
     CDS             2185..2385
                     /gene="infA"
                     /locus_tag="VHR64_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX3304305.1"
                     /translation="MTVDGKVVDALPNAMFRVELENGHTVLSHLAGKMRTNYIRVLPG
                     DRVTVELSPYDLTRGRITYRHR"
     gene            2539..3612
                     /locus_tag="VHR64_15585"
     CDS             2539..3612
                     /locus_tag="VHR64_15585"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX3304306.1"
                     /translation="MRSWRFLVGLLISAAFLFYAFRGQDYGAIVDSLKGVNYWLLIPA
                     LILYFLGVVARSFRWSVLLRPVKHIHTWDLLPITVIGFMANNVLPLRTGEVVRSYVLS
                     RTQGVRKTAALATIAVERIFDGLTMVGFMLVAALFVDFTSDLRHVMTVAVILFLIAIA
                     VLGVMTFAAGMRTRFIRFGVQLLPNTFAARIEHMAESFFSGLAALRSPRAVGLVALSS
                     VVAWTFEASMYLVIARAFGGSIKAVMDVPETLLTTGVANLATLVPSSPGYIGPFEAGI
                     KTVLEGALNASGSQALSYAILVHAALWFPVTVWGAVEWSRLHLSLKQVRAEEEAAASV
                     VATSGAHSEASGLKETVTRTNAA"
     gene            3622..4488
                     /locus_tag="VHR64_15590"
     CDS             3622..4488
                     /locus_tag="VHR64_15590"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX3304307.1"
                     /translation="MDTFEAVILGLVQGLTEFLPVSSSGHLLIVPWLFNWEQPPLAFD
                     AALHFGTLVAVVVYFRVELLRMLRAIPLAVRNLGPLLRGDAIDHPLAPDARLGLLIVI
                     GCIPGGIAGVIGNDAIDRFFHSEAHQDRAMLVVAFFLATFGILLWVAERVSKRSRPLS
                     NLKMADTIVIGLAQAIALMPGVSRSGVTLTAGLFRDLRRPDGARYSFLLGLPLILAAS
                     LTGIKDLTDSGSGEIGTTQIVIGISVSAVSGLAAIWGLLRLLQHAPTTMFTIYRLGLA
                     ALIVILIATGVR"
     gene            complement(4724..>5935)
                     /locus_tag="VHR64_15595"
     CDS             complement(4724..>5935)
                     /locus_tag="VHR64_15595"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX3304308.1"
                     /translation="AGVINAGFIFPNLDTLTTTDYGKVLIGKVIIVAVVLGLATYHHL
                     SLRGSLRALPSLMRNSVRVELGLVAVAIAFASTLALLAPPQVSHGELARIDLAMPTTP
                     ELTTDQVYVRLTIDPAKTGQNTLTTYATQGPPLTVETDASGAPQAVNHPPLTDVQLMR
                     IDLTSIDLAIAPRSVDLAPLGDGRFQTKGVNFSANGWWRAVVTVRRAGVAEDVSSEFI
                     VRVPDPNVVGFDQTAGKTSDPDAEALYTKAVLQFTQQTWSVLSQDLSGGNGGVEISNQ
                     VWSNGGLQIQTPQLQMIRLNGKRYLLDQSGQWRVTDDSDPGGPSTWVQEWDGATDFRL
                     GNQEQVAGQAAQVVHFYVPQTAGLAPAFYTLWIGTESGQILQEAMISRSHYMIERYDW
                     SKPPAQLVPPV"
BASE COUNT         1013 a         1930 c         1866 g         1126 t
ORIGIN      
        1 ggatcgaggt attgctcttg cagtcccgcg ccacgctcta ccacacgatc gagacctggc
       61 tcgactatcc cgaatcccgc ccgcagatcg gctggcaatt cgccgcgaca aagcatgcgg
      121 tgaccaacaa tgccgtcgag atcaccgatc tggcgatgcg gattgcgggc tcggccgggc
      181 agttccggcg ctacccgctg gagcggtatt tccgcgatgt ccgttcagga ctcggcaacc
      241 cgccgatcga tgacatcgcg ctcaccatcg tcggcaaggc agcactcggc atcggcagac
      301 gcaagtaaca ggagagacgg catgagcacc ctgacgtcgt atctggaacg aaagaccgtg
      361 gcactgaagg cggcgcgggc gagcgcatcg ccgaagcagg atgagcgcat gcagcacctg
      421 accgctcagt gcacggccga gggcggcagc ggcatccggc gggtccggat ccgcgacttt
      481 cagatcatca ccgatgccgg accggcgatg gccgggtacg acctcggccc gcgttcgccc
      541 gaggtcttgc tcggcgcgct gggcagctgc atctcgcaca ccgtcctgat ccaggcggcg
      601 gtcgagggaa tccccatcga gtcgctggta gtcgacgtct ctgccgacat cgattcgctc
      661 gccggaaaca ccgacgtgcg aacgccgttg aagaacatcg ccttcgagat ccaggtcgaa
      721 tccagcgcct caccggacgt tttcacgcga atcaacgagc tgctgccgga gatctgcccg
      781 gtcctgaacg tgatcacctg tccacagacc ataacctcgg ctgtcgtcca caacggcatc
      841 aaggtttgag cggacagatg ccggatgacg tgatcaacca tttcgccggg atcaggccgg
      901 ggagtccgct ctcaggactg cgcgcgcagc ggccagagac gctgcagcac gcgcagggaa
      961 gcttccaggc gttgcttgag ccggatgatc cgggcggcgt ctctcaagaa gagcgggagg
     1021 caatcgccct gcgcgtcgcg acgctcgagc ggttcccgga tattgccgcc ttccaccggg
     1081 agcgattgcg agagctcgat gcagacgacg aggtcgtttc cgccgtcgag cagttcccgg
     1141 atggcggcga tctgcccgcg cgactggcgg caatcctgcg ccataccgac atgctgacga
     1201 cctcgtcgcg cagtggctcg cccgaggcga tcgccgcact cagggaggcc gggctgtcgg
     1261 cgcgggagat cgtcacgatc agccaattga tcgccttcat gagttacgag atccggatga
     1321 tcgccctcct tcgggcgatg ggaggagcgc agtgagccca gccagtcgcc gtgccacgca
     1381 attcacgatc gagcggctcg actgggcgcc atggatcgag actgtgaccg agattggcct
     1441 ctctgacgag gagctagccg cggtctacga gcaagcaccg aacgccaagg gcagcccgta
     1501 tttcgcgacg cttgcgctgg atttgccggc attggtggag cgaaccggtc tcttcaacaa
     1561 caccatgcgc agtcccggcg gcggctccaa cgccgagcgg gaactggccg caacggcggt
     1621 ctcccggatc aacgggtgcg tctactgtgc atcggtccat gcccagcgct acgtgcagta
     1681 cgcgaagcgg ccagaggtca tggaccggct gctcgaagag ggcgtcgatg ctccgctgaa
     1741 cgatgcgcgg gagcaggcga tcgtcgattt ctcggccaag cttacccgga atcctgcggg
     1801 cttgacttca gacgatatca agccgcttcg ggtcgccggg ctaaccgacc tcgagatcta
     1861 cgacatcgcc caatcggccg cgatgttcgc ctgggccaac cgcttgatgc agacgctggg
     1921 agatgccgtc tacccccagt aggcagtggt cagtagccag ctctggagca cgctctgccg
     1981 ccctccaatc actaaccact agtcactatt cactcgatgc tagaattgac atatctgggg
     2041 ccgcacgggc ttgtgcggcg tgccatgaaa acagggagaa atgctttgcc gaagaagcca
     2101 gcctatcgta agccagtgcg caacacggcg cctgcgcctg gcacaaccac cgaggaacga
     2161 ccccgccgcc aggatcccga tgcgatcacg gtcgacggca aggtcgtcga tgcgctgccg
     2221 aacgcgatgt tccgcgtcga gctcgaaaac ggtcacacgg tgctcagcca cctggccgga
     2281 aagatgcgca ccaactacat ccgcgtgctg ccgggcgatc gtgtcacggt cgagttgtcg
     2341 ccgtacgacc tcacccgcgg ccgcatcacc tatcgacacc gctaacccgc ccgttgcggc
     2401 tttcgtgacc tccgggatat cgacccatcg ccttcatatc gacctatact cgatctgccg
     2461 cgcgacgata gcggcgtaat gcaacgcgcg gtctgggtgc gacgtgtccg tggctaagac
     2521 cggaaagctg tagagcccgt gcgctcctgg cgttttctcg ttggacttct gatcagcgcc
     2581 gccttcctgt tctacgcctt tcgcggtcag gattacgggg cgatcgttga ctccctgaag
     2641 ggagtcaatt actggctgct gatcccggcc ctgatccttt acttcctcgg ggtcgtggcg
     2701 cgctcgtttc gctggtcggt cctgctccgc cccgtcaagc acatccacac ctgggatctg
     2761 ctcccaatca ccgtgatcgg cttcatggcc aacaacgtcc tgccgctccg aacgggcgag
     2821 gtcgtccgct cctacgtcct gtcacgcacc cagggtgtcc gcaaaaccgc cgccttggcg
     2881 acgattgccg ttgagcgcat cttcgacggt ctgacgatgg tcggctttat gctggtggcc
     2941 gcgctctttg tcgacttcac gtccgacttg cggcacgtca tgacggttgc cgtgatcctg
     3001 ttcctgatcg cgatcgccgt gctcggagtc atgaccttcg ccgccggaat gcgcacccgc
     3061 ttcatccgat tcggtgtgca gctcctgccg aacacatttg ctgcccgcat cgagcacatg
     3121 gcggagtcgt tcttctccgg gctggccgcg ctgcgcagcc cacgagcggt cggtctggtc
     3181 gccctctcgt cagttgtcgc ctggacgttc gaggcatcga tgtacctggt catcgcgcga
     3241 gccttcggcg ggtcgatcaa ggcagtaatg gacgttcccg agacgctgct gaccaccggc
     3301 gtggccaacc tcgcgacgct ggtcccgtcg tcgcccggct atatcggtcc gttcgaggcg
     3361 ggcatcaaga ccgtgctcga aggcgcgttg aacgcatcgg gctcgcaggc gctgtcctac
     3421 gcgattctgg tccacgcggc gctctggttc ccggtgacgg tttggggtgc ggttgagtgg
     3481 tcgcgcctgc acctctcgct gaaacaggtc cgggccgaag aagaggctgc ggcgtccgtc
     3541 gtggcgacat cgggcgctca ttccgaagcg tccggtctca aggagacagt gacgcgtacg
     3601 aacgcggcat aattcgcgtt cgtggataca tttgaagccg tgatcctggg cttggtgcag
     3661 ggtctgacag aattcctgcc cgtctcgtca tccggtcacc tgctgatcgt tccctggctc
     3721 ttcaactggg aacagcctcc ccttgctttc gatgcggcgt tgcacttcgg cacgctggtc
     3781 gcggtggtcg tctatttccg ggtggaactg ctgcgcatgc tccgcgccat tcccctggcc
     3841 gtgcgcaacc tcggtccgct gctgcgcggc gacgcaatcg atcatccgct cgcgccggat
     3901 gcccgcctcg gtctgctgat cgtgatcggg tgcattcccg gtggcatcgc gggggtgatc
     3961 ggaaacgacg cgatcgatcg cttcttccac tccgaagcgc accaggaccg ggcgatgctc
     4021 gtagtggcct tcttcctggc cacgtttgga atcctgctct gggtggcgga gcgcgtctcg
     4081 aagcgcagcc ggccactgtc caacctgaag atggcggaca cgatcgtgat cggtctggcg
     4141 caggccatcg ccttgatgcc gggcgtgtcc cggtcgggag taaccttgac cgccggcctg
     4201 ttccgggact tgcggcggcc cgatggcgcg cggtactcgt tcctgctcgg tctgccactg
     4261 atcctggccg cgagcctgac cggcatcaag gacctgacgg attccggctc tggcgaaatc
     4321 ggcacgaccc agattgtgat cggaatctcc gtcagcgcgg tctcgggcct ggccgcgatc
     4381 tgggggctcc tcagactcct ccagcacgcg ccgaccacca tgttcacgat ctaccgcctc
     4441 ggtctggcgg ccctgatcgt gatcctgatc gcaacgggcg tccgctagac cggcacttgg
     4501 tttcgactgc ttgcacctga cgtagcgcgg agttaccacg ccccgcccaa gccccccggc
     4561 agccgaatcg tgtcggaaac cagccccaca gccggactag ctccgttatt cgtgcgtggg
     4621 gatacaccaa gcggggcatg ttcctctctc cggctctctc ctcaaggaaa gagggcatcc
     4681 ccgcgctagg tttgccggac tgtcagcctt tgacagcgtc gcgtcaaacc ggtgggacga
     4741 gttgagctgg cggtttggac cagtcgtacc gctcgatcat gtagtgactg cgggagatca
     4801 tcgcctcctg caggatctgg ccactttccg tgccgatcca gagggtgtag aaggcaggcg
     4861 ctaaccccgc tgtttgcggc acgtagaagt ggacaacttg tgcggcctgg ccagcgactt
     4921 gctcctggtt gccgagacgg aagtcggtcg ctccgtccca ctcctggacc catgtcgacg
     4981 gtccgcccgg atcggagtcg tcggtgacgc gccattggcc gctctgatcg agcagatacc
     5041 gtttcccatt gagccggatc atctgaagct gcggtgtctg gatctggagc ccgccgttcg
     5101 accagacctg gttcgagatt tcgactccac cattgccgcc ggagagatcc tggctcagga
     5161 ccgaccaggt ctgctgcgtg aactgcagca ccgccttggt ataaagcgcc tcggcgtccg
     5221 gatcgctcgt ctttcccgcg gtttgatcga agccgaccac attcggatcc gggacgcgga
     5281 cgatgaactc ggacgacaca tcctcggcca cgcctgctcg ccggaccgtg acgaccgcgc
     5341 gccaccagcc gtttgcgctg aagttgacac ccttcgtctg gaaccggccg tcgccgagcg
     5401 gcgcgaggtc aaccgagcgc ggcgcgatcg ccaggtcgat cgaggtcagg tcgatccgca
     5461 tcaactggac atcggtcagc ggcggatgat tgaccgcctg cggggcgccc gatgcgtcag
     5521 tctcgacggt caacggtggc ccctgggtcg catatgtcgt gagcgtgttc tggccggtct
     5581 tggccgggtc gattgtcagg cgaacgtaga cctggtcggt tgtcagctcc ggcgtagtgg
     5641 gcatggcgag gtcgatcctg gcgagctcgc catgggagac ctggggaggc gcaagcaggg
     5701 ccagggtcga agcgaaggcg atcgcgaccg cgacaagtcc caactcgacc cggaccgagt
     5761 tgcgcatcag cgacgggagc gccctgagcg agccgcgcag gctgagatgg tgatatgtgg
     5821 cgaggccgag caccaccgcc acgatgatca ccttgccgat cagcaccttg ccatagtcgg
     5881 ttgtggtcag cgtatccagg tttggaaaga tgaatccggc attgattacc ccggc
//
LOCUS       DATAOZ010000626         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4716449, whole genome shotgun sequence.
ACCESSION   DATAOZ010000626 DATAOZ010000000
VERSION     DATAOZ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4716449"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VHR64_15600"
     CDS             <1..1372
                     /locus_tag="VHR64_15600"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX3304309.1"
                     /translation="HRIWYHRTTGIWQPVWLEQVADVHVEELRWSFDLTRSALDMSVR
                     LNRKPADSFRLRIRLSLHDQLLADDTYFVEGDRLIRAIAIDSGQAMMRPEVTLWSPEC
                     PNLIDAELTLLDGDTVVDRVESYTGLRSCGAANGRFLLNGRPYYTRMVLEQGYWPESH
                     LAAPSGDALRREVELIRALGFNGVRIHQKVEDPRFLYWCDRIGLLVWGEMANAYDYSP
                     DGVARFTREWLEVIRRDVSHPCIVTWVPVNESWGVLNLEGDPAQREYVKALAHLTRAL
                     DPTRPVISNDGWEHLASDILTIHDYTHDAERIRERYGSRDALARTIREVQPAHRRISM
                     TGANLADEPVLLTEFGGINFATDQDRPHWGYSSAHDAGSFLAAYGELVSAVLDSTDLA
                     GYCYTQLTDTMQETNGLLTENREPKVDIAAIHALNTQPSRSMPAEAVRAAQKASESAT
                     TSKTQA"
     gene            1393..2592
                     /locus_tag="VHR64_15605"
     CDS             1393..2592
                     /locus_tag="VHR64_15605"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX3304310.1"
                     /translation="MRITGATTYIVGNPWKNWVFVRLDTDQPGLYGVGEGSLNGFAKS
                     VDAAIHELAPRYEGMDPFQVETIYQRMARDLYSDGGQIHMNAAAAIEIACWDIIGKET
                     GRPVYDLLGGQYHERLPVYANGWYQGERKPEMFAEKAKDVVARGYKALKFDPFGSAWR
                     TMTQEDRHLSLKIVEAVRDAVGPDIQLMIEGHRRFSVAEAIKIGHELTQYDPTWFEEP
                     TDHAKIDATAQVAASLPIPVSAGESFTSTHQFAELLAHNTVHILQPDPSNLGGILKTR
                     MVCGMVDAWYGVVAPHQAQGPISTAVCVQIDACTPNLLVQELFDEFNVDWERDIVTWH
                     PTLAEDGTIDVPTAPGLGCDLVFSELEKHPYQSSNFLPLFAPGWEKREGERPSVPAGD
                     QDAEPVR"
     gene            2589..>3403
                     /locus_tag="VHR64_15610"
     CDS             2589..>3403
                     /locus_tag="VHR64_15610"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX3304311.1"
                     /translation="MTDLHGCIPILCTPFFDDFSVDFESLERQIDWVIAEGASGVATL
                     ALASEGYKLTEGERDEITRVVVAKTAGRVPVVVSGDGGSPDVAIDRALRAQRAGATAL
                     MVLPASFVKPGPAALRDYYMRIGRAVEIPLIIQDAPQLTGVAMSPALWADLAREVETI
                     QYVKAEGVPQGQTLTATLEQSEGRLRVFCGWGGLSMIDAMERGAAGSMPAANFTRFFA
                     EIQRRFEDGDVAGAEALFQSELPYVLWAMQSIDFSVRATKTELLARAVFRTRT"
BASE COUNT          621 a         1119 c         1093 g          570 t
ORIGIN      
        1 gcaccggatc tggtaccacc gcacgaccgg catctggcag cccgtctggc tcgagcaggt
       61 ggcagacgtg catgtcgagg agctgcgctg gtcgttcgac ctgacgcggt cggcgctcga
      121 tatgtccgtc cgtctcaatc ggaagcctgc cgactcgttt cggctccgga tccgcctgtc
      181 cctgcacgac caactgctcg cggacgatac gtacttcgtc gaaggcgatc ggctgatccg
      241 tgccatcgcg atcgattccg gccaggcgat gatgcgcccg gaagtgacgc tctggtcccc
      301 ggagtgcccg aacctgatcg atgctgagtt gacgttgctg gatggcgaca cagtcgttga
      361 ccgtgtcgag agttacaccg gtctgcgctc ctgcggcgcg gcaaacggtc gattcctgct
      421 caatggccgc ccgtattaca caaggatggt gctggagcag ggctattggc cggagtcgca
      481 cctcgctgcg ccgagcggag acgcgctgcg gcgtgaagtc gagctaatca gggcgctcgg
      541 cttcaacgga gtccgcatcc atcagaaggt cgaggacccg cgcttcctct attggtgcga
      601 tcgaatcgga ctgctcgtct ggggtgaaat ggccaatgcc tacgactact ctcccgacgg
      661 cgtcgcgcgg ttcacccgcg agtggctgga ggtgatccgg cgcgacgtca gccatccctg
      721 catcgtgacc tgggtgccgg tgaacgagag ttggggcgtg ctcaacctcg aaggcgatcc
      781 cgcccagcga gagtatgtga aggcgctcgc ccacctcacc cgcgcgctgg atccgacccg
      841 gccggttatc agcaacgacg gctgggagca tctggccagc gacatcttga ccatccacga
      901 ctacacgcac gacgccgagc ggatccgaga gcgatacggc tcccgtgacg cgctggcgcg
      961 gacgatccgc gaggtgcagc ccgcgcatcg ccgcatctcg atgaccggtg cgaatctggc
     1021 cgatgagccg gtcttgctca cggagttcgg cggtatcaat ttcgcgaccg atcaggaccg
     1081 acctcactgg ggatacagct cggcgcacga cgccgggtcg ttcctcgcgg catacggcga
     1141 gctggtctcg gcggtcctcg actcgactga ccttgccggc tactgctaca cgcagctaac
     1201 cgacaccatg caggagacga acggcctgct gaccgagaac cgcgagccga aggtcgatat
     1261 cgcggcgatc cacgcactca acacccaacc cagccgctca atgcccgccg aggcggtgcg
     1321 cgccgcccag aaagcgtccg agtcggctac gaccagcaaa acgcaagcct gaatcagggg
     1381 aaggagcagt ccgtgcggat caccggcgcg acgacttata tcgtcggcaa cccgtggaag
     1441 aactgggtct ttgtccggct cgataccgat cagccgggtc tctacggcgt cggcgagggg
     1501 tcgctgaacg gcttcgcgaa atcagtcgat gcggcgatcc acgagctggc gccgcgctac
     1561 gaggggatgg acccgttcca ggtcgagacg atctaccagc ggatggcacg tgacctctat
     1621 tcggatggcg gccagatcca catgaacgcg gccgccgcga tcgagatcgc ctgctgggac
     1681 atcatcggca aggagaccgg gcggccggtt tacgacctgc tcggcgggca gtaccacgaa
     1741 cgactgccgg tctatgccaa cggctggtac cagggcgagc gcaagccgga gatgttcgcc
     1801 gaaaaggcga aggatgttgt cgcacgcggc tacaaggcac tgaagttcga tccgttcggc
     1861 tctgcctggc gcacgatgac gcaggaagac cggcacctct ccctcaagat cgtcgaggcg
     1921 gtgcgggacg cggtcgggcc ggatatccag ctcatgatcg agggccatcg ccgcttctcg
     1981 gtagccgagg cgatcaagat cggccatgag ctaacccaat acgacccgac ctggttcgag
     2041 gagccgaccg accacgccaa gattgatgcg acggcgcaag tcgcggccag tctcccgatc
     2101 cccgtctcgg ccggcgagag cttcaccagc acgcaccaat tcgcggagct gctggcccac
     2161 aacacggtcc acatcctgca gccagaccca tccaacctcg gcgggatcct caagacccgg
     2221 atggtatgcg ggatggtcga tgcctggtat ggcgtcgttg cgccgcacca ggcgcagggg
     2281 ccgatctcta ccgccgtctg cgtccagatc gatgcctgca caccgaatct gctcgtgcag
     2341 gagctattcg acgaattcaa cgtcgactgg gagcgggata tcgtcacctg gcacccgacc
     2401 ctggccgagg acggcacgat cgatgtcccg actgctccag gtctcggttg cgacctcgtc
     2461 ttcagcgaac ttgagaagca tccgtaccag tccagcaatt tcctgccgct cttcgcaccc
     2521 ggctgggaaa agcgcgaggg cgagcggcca tcggtccccg ccggcgacca ggatgcggag
     2581 ccggtccgat gactgatctg cacggctgca tcccgatcct ctgcaccccc ttcttcgacg
     2641 acttctcggt cgacttcgag agcctcgagc ggcagatcga ctgggtgatc gccgagggcg
     2701 catccggcgt cgcgaccctg gcgctcgcat ccgagggata caagctgacc gagggcgagc
     2761 gtgacgagat cacccgggtc gtggtcgcca agacggctgg gcgcgtaccg gtggtggtct
     2821 caggtgatgg cggcagtccc gacgtcgcga tcgaccgcgc gctccgggcc caacgagctg
     2881 gtgccactgc cctgatggtg ctgccggcgt cgttcgtcaa accgggaccg gcggcattgc
     2941 gcgactacta catgcggatc ggccgggcgg tcgagattcc actcatcatc caggatgcac
     3001 cgcaactgac cggcgttgcg atgtcccccg cactctgggc cgatctcgcc cgcgaggtcg
     3061 agacgatcca gtacgtcaag gccgagggcg tgccgcaggg ccagacgctg accgccacgc
     3121 tcgagcaatc cgagggccgg ctgagggtct tctgcgggtg gggcggactc tcgatgatcg
     3181 acgcgatgga gcgcggtgcg gccggatcga tgcctgccgc caacttcacc cgcttcttcg
     3241 ccgagatcca gcgccgcttc gaagacggtg atgtcgccgg agccgaagcg ctcttccagt
     3301 cggagctgcc ctacgtcctc tgggcaatgc agtcgatcga cttctcggtc cgtgcgacca
     3361 aaaccgaact actggcccgg gccgtattcc gaacaagaac cat
//
LOCUS       DATAOZ010000627         4366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5507057, whole genome shotgun sequence.
ACCESSION   DATAOZ010000627 DATAOZ010000000
VERSION     DATAOZ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5507057"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VHR64_15615"
     CDS             <1..901
                     /locus_tag="VHR64_15615"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX3304312.1"
                     /translation="GILAATSNRAVSRFVQQYGMRLGASRFVAGETFDEAIPVLRGLN
                     QKGLRTNTTLLGEGVKDPATTRSVVAEYKSDLDRIATEKLQTNIALKLTHLGLDLDPE
                     LAYRNVSEIASHAASHGNFIRIDMEESARVDDTLRIYRRLRGDGHDNVGTVLQSYLYR
                     TPDDLESILPLGPNLRLVKGAYLEPANIAYPRKADVDRAYLQLAERMLMDGSFTAIAT
                     HDERIIDHVIDFTRRHGIGNDRFEFQMLYGVRPQYQVELARAGHRLLVATPYGPHWYL
                     YLMRRLAERPANLAWFATNMLRR"
     gene            1109..2011
                     /locus_tag="VHR64_15620"
     CDS             1109..2011
                     /locus_tag="VHR64_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3304313.1"
                     /translation="MASVMAASPATAQPGHDPRDGTERRPTLAPAVAFANQKGGVGKT
                     TTAVNASVSLAQRGYRVLLIDADPQGNATSSLGIDKLGVGSSIYDVLIEDLPLAEVAT
                     ATDRPGLDVVPSTPMLAGAEIELVDAPGRETRLRQALTDLLSHYDVVLIDCPPSLGLL
                     TVNALTAARSVVVPIQCEFLALEGLGQLMTTVDRVKRGLNPSLDVTGVLMTMFDVRTR
                     LSAHVVEEVRRYFPHRMFSTVVPRSVRLAEAPSYGQAIVEYDPTSRGAEAYESFVGEL
                     GVRLNLPPRPRPQSISTHAEGTET"
     gene            2008..3576
                     /gene="guaB"
                     /locus_tag="VHR64_15625"
     CDS             2008..3576
                     /gene="guaB"
                     /locus_tag="VHR64_15625"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX3304314.1"
                     /translation="MTITQPHRIAITGIGDTAHWDDDFHQLEESSETDKFVGLGLTFD
                     DVLLVPAASNVLPANVDTSTHLTKKISLAIPLLSAAMDTVTEGRMAIALAREGGLGVI
                     HRNLSIEDQVTEVDKVKRSESGMIVEPVTLPPDAPVREALAVMERYHISGVPITEECG
                     KLVGILTNRDLRFVEDIEQPVSGVMTSSDLITAPAGTTLEEARSILHRHRVEKLPVVD
                     ERGYLTGLITVKDIQKKIQFPNATKDEKGRLRVAAAVGVGADADERAAALVAGGVDMI
                     VVDTAHGHAASVIQTVTRLKRRFDVAVMAGNVATAAATEALIDAGADAVKVGVGPGSI
                     CTTRVVAGVGVPQVTAVLNCVAAAARHDVPVVSDGGIQYSGDIAKAIAAGADAVMLGS
                     LLAGVDESPGEVVLFQGERFKEYRGMGSIGAMKSRSYSKDRYFQEGIGNIQKLVPEGI
                     EGRVAYKGPLGNLVYQLVGGLRSGMGYVGAPNIEALKRDSRFVRITAAGLRESHPHDI
                     VITREAPNYGSSGF"
     gene            4046..>4366
                     /locus_tag="VHR64_15630"
     CDS             4046..>4366
                     /locus_tag="VHR64_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304315.1"
                     /translation="MVSPAAAHSIGAVGADFEPPTYVQQRSLSCEYASLVIAFSTYGV
                     SVSEYAFDDLVGWSENPHLGFRGDINGNWGNTTDYGVYAEALVRPVTKFGFRGVAFYG
                     QGDAT"
BASE COUNT          858 a         1328 c         1320 g          860 t
ORIGIN      
        1 tggcatcctt gcggcaacca gcaaccgtgc cgtctctcgc ttcgtgcaac agtacggcat
       61 gcggctcggt gcttctcgtt tcgtcgccgg tgaaaccttc gacgaggcca ttcccgtgct
      121 ccgcggactc aatcagaaag ggctgcgcac gaatactacg ctcctcggtg agggagtcaa
      181 agatcccgct accaccagat cggtcgtcgc cgagtacaag agtgatctgg accgcatcgc
      241 gactgagaaa ctgcaaacga acattgcgct gaaattgacg caccttgggc tcgacctcga
      301 tccagagctt gcctatcgca acgtctcaga gattgccagc cacgccgcga gccatggcaa
      361 cttcattcgc atcgacatgg aagagtcagc ccgcgtcgac gacacactac gcatctaccg
      421 tcgtttgcga ggggatggtc acgacaacgt cggcaccgtc ctccaatcgt atctctatcg
      481 cactccagac gacctcgaat cgattctgcc attgggccca aatctacggc tggtcaaagg
      541 ggcctacctg gagccagcga acattgccta tccgcgcaag gctgacgtcg atcgcgccta
      601 ccttcaactg gccgaacgca tgctgatgga cggcagcttc acggctatcg ccactcacga
      661 cgagcggatc atcgaccacg tgattgactt tacacggcgc catggcatcg gcaacgatcg
      721 cttcgagttt cagatgctct acggggttcg cccgcaatat caggttgagc tcgcccgagc
      781 cggccaccgg ctactcgtgg caacgcccta cgggccacat tggtacctct acctgatgcg
      841 gcgcctcgcc gaacgccccg ctaacctcgc ctggttcgca acgaatatgc tgcggaggtg
      901 aggagtcgtg agtcgtgagc gagtcgagaa gtcgagtagt agaagaagac ggcaagacgg
      961 ctagaagacc taacaccctg tcacgctata accggccgaa ggccaacagc tgatagctaa
     1021 ttgccaatag ccaatagccg atagctcgca acggacaggc agtgctacca tcgccgcgag
     1081 gagcaagcgc actgcggacg agacgtgcat ggcgagcgta atggcagcga gtccggcgac
     1141 ggctcaaccc gggcatgacc cgcgggatgg caccgagcgc cggccgacac tcgcgccggc
     1201 cgttgccttc gccaatcaaa aaggtggcgt cggcaagacg accaccgccg tcaacgcctc
     1261 ggtctcgctg gcccagcgtg ggtatcgcgt cttgctcatc gacgccgatc ctcaaggtaa
     1321 cgcgacgtct tcacttggca tcgacaagct tggcgttgga tcctcgatct atgacgtcct
     1381 gatcgaggat cttcccctgg ctgaggtggc cacggccacc gatcgaccgg ggctcgatgt
     1441 cgttccctca acaccgatgc tcgctggcgc cgagatcgag ctcgtcgatg cccccggcag
     1501 agaaactcgc ttacggcagg ctctgacaga tttgctgtcc cattacgacg tcgtgttgat
     1561 cgactgtccg ccttcgctcg gtttgctaac ggtcaacgcc ctcacggcgg cgcgttcggt
     1621 ggtagtgccc atccagtgcg agtttttggc cctggagggc ctgggtcagc tcatgaccac
     1681 tgtcgatcgc gtgaagcgag gtctcaaccc ttcgctcgac gtaaccgggg tgttgatgac
     1741 catgtttgac gttcgcaccc gcctttccgc ccacgttgtc gaggaagtgc ggcgatattt
     1801 cccacaccgc atgttcagta cagtggtacc gcgatcggtc cgattggccg aggcgccatc
     1861 gtatggtcag gccatcgtcg agtatgaccc tacctcccgt ggcgctgaag cctacgagtc
     1921 attcgtaggc gagctcggtg tgcggctcaa tttgccccct cgaccccgcc cacagtccat
     1981 ttccacgcat gcggagggaa ccgaaacatg accatcaccc aaccacatcg cattgcaatc
     2041 acgggcattg gcgatactgc ccactgggat gacgactttc atcaactcga agagtcaagc
     2101 gaaaccgaca aattcgtcgg actgggactg accttcgacg acgtcctgct ggtgccggca
     2161 gcatcgaatg tgttgccggc aaacgttgac acgtcgactc acttgacgaa gaaaataagt
     2221 ctggccatcc cgctcctctc ggccgccatg gacaccgtga ccgaagggcg catggccatt
     2281 gccctggcac gagagggtgg cctgggcgtc attcatcgca atctttcgat cgaggatcag
     2341 gtgactgagg tcgacaaggt gaagcgctcg gaatcgggca tgatcgtgga gccggtcacg
     2401 ctaccacccg acgccccggt tcgtgaggcg ctggcggtca tggagcgcta tcacatttcc
     2461 ggcgtcccca tcacagaaga atgcgggaag ttggtcggca tcctgacgaa ccgggatttg
     2521 cggtttgtcg aggacatcga acagcctgtc agcggggtga tgacgagcag tgatctgatt
     2581 acggcccccg ccggtacgac cctggaggaa gcgcggtcga tcttgcatcg gcatagagtc
     2641 gaaaagttac ccgtggtcga cgagcgtggg tatctgactg gacttattac ggtcaaagac
     2701 atccagaaga agattcagtt cccgaatgcc accaaggacg agaagggacg cctgcgtgtc
     2761 gcggccgcgg tcggtgtcgg cgccgatgcc gacgagcgtg ctgctgccct ggtggccggt
     2821 ggcgtagaca tgatcgttgt cgataccgct catggtcacg ccgcctccgt catccaaacg
     2881 gttacccggc tcaaacggcg ctttgacgtc gccgtgatgg cgggcaacgt cgccaccgct
     2941 gccgcaactg aagccttgat cgatgcgggt gccgacgcgg tcaaagttgg cgtgggtcca
     3001 ggctcgattt gcaccacccg ggtcgtagca ggcgtcggcg tccctcaagt gaccgcggtg
     3061 ctcaattgcg tcgcggccgc cgccaggcat gacgtgccag tcgtctcgga cggcggtatc
     3121 caatattctg gagacattgc caaagcgatt gccgcgggag ccgacgccgt gatgctgggg
     3181 tcgctcctcg ccggagtcga cgagagtccg ggcgaggtgg tgctctttca gggggagcga
     3241 ttcaaggaat accgcggcat gggctcaatt ggggccatga agtcgcgctc ctattctaag
     3301 gaccgctatt ttcaagaagg catcggcaat atccagaagc tggtccccga agggatcgag
     3361 ggacgagtag cctacaaagg acccctgggc aacctcgttt accagctcgt cggcggtctg
     3421 cgctccggca tgggttacgt cggggcgccg aacatcgagg cgctgaaacg tgactcgcgc
     3481 ttcgtacgca ttaccgccgc cggtttgcgc gagagccatc cccatgacat cgtgatcacc
     3541 cgcgaggcgc ccaactacgg gtcgagcggc ttctagcccc ttcacacaga gctactgcta
     3601 acagcggtcc gtcgagatct cgattgaggc atgaccgcca ggcggtcttc cagcgtcgaa
     3661 ggcccggcgg agcattcgcc ggccctggag ccggcattgg aagcctgatc gttggcattt
     3721 gccgaaaaaa ggtacgaaca gctccctgtg acgtattccg acgcccgggc cgcgctgtct
     3781 agcatgcttg cgtaaccatc aggccacatc gttcgtttca ctttacgacg aagatgtggt
     3841 gcgttgcacg ggacgcggct cgcgtgaaat gttgaacgtc ttgcggcgtc acgagaggcg
     3901 tcggtttgac ttcttgaaca gagtggtgct tcggcgggag gggaagccgg cgatgcacgg
     3961 agtcatgaga agggcagcga tacgggtcag atcttcgctg gcggtagtgc tttgcgccct
     4021 tgttgtcgtt tcaatgactg gcatcgtggt gtcgcccgct gccgcgcatt cgattggggc
     4081 agttggggcg gatttcgagc cgccgacgta cgttcagcag cgcagcctct cctgtgagta
     4141 cgcctcgctc gttatcgcct tcagcacgta cggtgtctcc gtctcagaat acgcgtttga
     4201 tgacctcgtc ggctggtccg agaatccaca cctcggtttt cgcggtgaca tcaacggcaa
     4261 ctggggtaat acgaccgact acggtgtcta cgccgaggcg ctggtgcggc ctgtcaccaa
     4321 gttcgggttc cgaggggtgg ccttttacgg tcaaggcgat gccacg
//
LOCUS       DATAOZ010000628         5503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5507524, whole genome shotgun sequence.
ACCESSION   DATAOZ010000628 DATAOZ010000000
VERSION     DATAOZ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5507524"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1956
                     /locus_tag="VHR64_15635"
     CDS             196..1956
                     /locus_tag="VHR64_15635"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX3304316.1"
                     /translation="MNEVGRAERVQEPEHGRRDRVKDAVARARSRPLPRVEKSDLAAG
                     LTSGIANIPDGMASAVLAGVNPVFGIYTVIVGTPVASLAISTQLMMFNTTSAMTLVAV
                     DGLGSRKGADRIDALIAVALVAGLIQIALGLLGLGMLTKFVPNSVMVGFLTGIASLVI
                     LGQLWDLTGYHDNLGGNKLEKTWRLIAHFDQVDLWTTALGLGCLVALVALSRGRLANF
                     NLLIALALVTLVAWIFAPDSVVLVSSLGEIPRDLPSFDLPKFSLVPSMLLAGIAVGIV
                     GVLQAAGVAQGYPNPDGSETSDSRDFVGQGIANTVGSCFRAMAGGGSLSGTALFVSAG
                     ARTRIAGVIQAVVVVVLVLAFSDLLGFIPMAGLAALLIYAAALSIRFPAIATIQRTSA
                     TSVVTMLATLLATLVIPLQQAVIVGVVFAAILFVYRASVDVRVSELTIHGERLSFGPP
                     PTQLRSNDVTILDIQGNLFYAGARTLGRLLPPATGVERPVVVLRIRGQRDLGSTFFKV
                     AGAYAQRIADCDGRLILAGVEPVVLKRLERTGMTATIGAGNVFAADTILGDSVLAAYT
                     AGRAWLDGQQLVSTTVAAPE"
     gene            complement(1985..3736)
                     /gene="sulP"
                     /locus_tag="VHR64_15640"
     CDS             complement(1985..3736)
                     /gene="sulP"
                     /locus_tag="VHR64_15640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEX3304317.1"
                     /translation="MVLDVHADVNESARATRGNRVGRLLPIIGWARQYDRSWLRSDVI
                     AGVTVTALVVPKNLGYAEIARVPIENGLYAAAAGAILYGVFGTSRQISTGPSSALAAV
                     AGSAVLATGISSEPDAAAIVAAIAVGAGLLFILLSVLQMGWIAQFISRAVITGFLFGA
                     AIDVVTGELPKITGSDGSGDNAWEKFWSWLKGLDGIDGTTLLVGSLSLAALFGLRAVS
                     PKFPGALVVVIGGLLASALFDLGDNGVALVGDVPRGLPSLVIPDLGLVADHALLVLVA
                     SVAIVMIGFSQSAGDARYFAAKNAYRVDINQESLAQGAANVGAGLFQGIPVSTSLSAS
                     SLNDSAGAKSQVATLTTGAAVVLTLIIFAPLFSDLPKAVLGAVIIEAVTTGMMDFPEL
                     RRLFVVKRSDFWIAMAAITGVVLAGVLAGVVIGVVLSLIWLLRVATSPAMPHLGRVKG
                     THVFREIDEHPDDERIPGVVALRLEGALFFVTADSLEDRIEELVGAAESPASVVVLDF
                     QSVYIIDSQGAGKLGEIADNLRAKGLSFQFARVKPAVREVLERDGLVEQIGPENFFLD
                     VNQAVEAYLAQAPKSFQ"
     gene            complement(4014..4895)
                     /gene="ppk2"
                     /locus_tag="VHR64_15645"
     CDS             complement(4014..4895)
                     /gene="ppk2"
                     /locus_tag="VHR64_15645"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX3304318.1"
                     /translation="MATVKHKKDKTHKHQHQDDGLVMASSAGAENPESEQPRMSAREF
                     EKALKPLQIELVKLQAWVKATGAKVCIVFEGRDAAGKGGTIKRIRERVSPRVFPVVAL
                     PAPTEREKSQMYVQRYVPHFPAAGEVVIFDRSWYNRAGVERVMGFATEQQVDEFLSHV
                     IPFERAMIASGIILIKYWLEVSQEEQLRRFERRISDGRKTWKLSPMDLESCARWYDYS
                     RARDAMFAATDTADSPWHVVDSNEKRRGRLNCISHLLSLVPYEEIPVLDVTLPERRPA
                     EGYVEPDYPYRYVPMRY"
BASE COUNT         1047 a         1780 c         1659 g         1017 t
ORIGIN      
        1 ctctacgaca cctctcatcc aacggttctc cccaccaagg accaataacg aaagactcgc
       61 ggcggcgcgc tggtcgttcg tcgcccaccg atcgcgctgc aacacatcag ccactgattg
      121 acaagcgagg agtactctca gagtatcgtc gtctcaagta cgctcgttac tccttcctaa
      181 aggagtgtcg ccacggtgaa cgaggtcggc cgagccgagc gtgtgcagga gccggagcac
      241 gggcgcaggg accgtgtgaa agacgcggtc gcgcgcgccc gctcccgacc gctgccacgc
      301 gtcgaaaaga gcgatctggc ggccgggctg acgtcgggca tcgccaatat ccccgacggc
      361 atggcctccg cggtactcgc cggtgttaat ccggttttcg gcatctatac ggtgatcgtg
      421 ggaacaccgg tcgcatcact cgcgatctcc acccagctca tgatgttcaa caccaccagc
      481 gcaatgacgc tcgtggcggt cgacggattg ggatcacgga agggggccga tcggattgac
      541 gccctgattg ccgtggccct ggtcgccggt ctgattcaga ttgcgcttgg cctgctcggg
      601 ctcggcatgt tgaccaagtt cgttcccaac tcggtcatgg tcgggttcct caccggcatc
      661 gcatcgttgg tcatcctggg gcagctttgg gatctgacgg gctaccatga caatctcggg
      721 ggaaacaagc tcgagaaaac gtggagactt attgcccatt tcgatcaggt cgatctgtgg
      781 acaaccgcgc ttggcctggg ctgcctggtg gcattggttg cgttgtctcg cgggaggctc
      841 gcgaacttca atctcctgat cgccctcgcg cttgtgacgc ttgttgcctg gatcttcgcg
      901 cctgactccg ttgtcctggt gagcagcctg ggggagattc cacgcgattt accatcgttt
      961 gacctgccga agttctcgct tgtccccagc atgctgctgg cgggcattgc ggtcgggatc
     1021 gtcggagtct tgcaggcggc aggcgtggcc cagggttacc caaaccccga cggcagcgaa
     1081 acgagcgact cccgggattt cgtcgggcag gggatcgcca acacggtcgg ctcctgcttt
     1141 cgtgcgatgg ccggtggcgg atcgctctcc ggtaccgcgc ttttcgtgag cgccggtgca
     1201 aggacacgga tcgcgggagt catccaggcc gtggtcgtcg tcgttctcgt cctggcattc
     1261 agcgatctgc tgggcttcat tccgatggcc ggactggctg cgctgctcat ctatgccgcc
     1321 gcgctttcca tcagatttcc tgcgatcgcg accatccagc gcacctcggc aacatccgtc
     1381 gtcacgatgt tggcaacctt gctcgcaacg ctggtgattc cattgcagca agcggtgatc
     1441 gtcggtgtgg tctttgccgc catcctgttc gtgtaccgcg ccagtgtgga cgtccgggtg
     1501 agcgagttga cgatccacgg cgagcggctc agcttcggtc caccaccgac ccagctgcgt
     1561 tcaaacgacg tcactatcct cgacattcag ggtaacctct tctacgccgg tgcccgcacc
     1621 ctcgggcgcc ttctgcctcc ggcaaccggc gtggagcgac cggtcgtcgt actccggatc
     1681 cgtggtcagc gggatctcgg cagtacgttc ttcaaggttg ccggcgccta tgcccaaaga
     1741 atcgccgact gcgacggtcg cctcatcctg gccggagtcg agcccgtcgt cctcaaacgg
     1801 ctggaacgga ccgggatgac cgcgacgatc ggcgccggca acgtcttcgc agccgacacc
     1861 atcctgggtg actcagtgct cgccgcctac actgccggac gagcctggtt ggacggccag
     1921 caattggtga gcacaaccgt ggcggcacca gagtagaagc gtgctggcac ggaacgcccg
     1981 cggttcattg gaatgatttc ggcgcctgcg ccagatacgc ctcgactgcc tggttgacat
     2041 cgaggaagaa gttttccggt ccgatctgct cgacgagccc gtcgcgctca agaacctccc
     2101 tcaccgcagg cttcaccctg gcaaattgga acgacaggcc cttcgcgcgc aggttgtcgg
     2161 caatctcgcc gagcttgcca gccccttggg aatcgatgat gtacacgctc tggaagtcaa
     2221 gaacgacaac gctggccggg gactctgccg cgccgaccag ttcctcgatg cgatcttcga
     2281 gggaatcggc cgtcacgaag aacagcgctc cttcgagtcg aagggcgacc actccgggaa
     2341 tccgctcgtc gtcgggatgc tcatcgattt cgcggaagac atgggtaccc ttgacgcgac
     2401 ccagatgggg cattgccggc gaggttgcca cacgcagtag ccaaatcagc gagagcacca
     2461 cgccgatgac gacgccggca agcactccgg ccagcaccac cccggtgatc gcggccatcg
     2521 cgatccagaa atcggaccgc ttgacgacga agaggcggcg aagctcaggg aaatccatca
     2581 tcccggtcgt aaccgcttcg atgatcacag caccgaggac ggccttggga agatcggaga
     2641 agagcggcgc aaagatgatc aacgtcagaa caaccgccgc gccggtcgtt agtgtggcaa
     2701 cctgcgactt tgctcccgca ctgtcattca gcgaactggc ggagaggctc gtcgacactg
     2761 ggatgccctg gaatagcccg gcgcccacat tcgctgctcc ctgggcgagc gactcctggt
     2821 tgatgtcgac ccggtacgcg ttcttggcgg cgaaataccg ggcgtcgccg gcgctctgcg
     2881 agaagccgat catgacgatc gctaccgagg caaccagcac cagtaatgcg tgatccgcta
     2941 ccaggccgag atccggaatc accagcgaag gcagcccccg cggcacgtcg ccgaccagcg
     3001 ccacgccgtt gtcgccgagg tcgaacagcg cggacgccag gagtccgccg atcaccacca
     3061 cgagtgcgcc cgggaacttt ggagacactg cgcgcagccc aaacagggct gccagcgaaa
     3121 gcgacccgac gagcagcgtg gttccgtcga tcccatcaag tcccttgagc caggaccaga
     3181 atttctccca ggcgttatct ccactaccgt cactgcccgt gatctttggc agctctccgg
     3241 tgacgacgtc gatcgccgct ccgaagagga agccggtaat gacggcgcgg gagatgaact
     3301 gggctatcca ccccatctgc aggacagaca gcaagataaa gagcagcccg gcgcccacgg
     3361 cgatcgcggc cacgatcgcg gctgcgtccg gttcgctcga gatgccggtt gcgaggacgg
     3421 cgctaccggc aacggccgcc agagcggagc tgggtccggt tgaaatctgc cgcgaggtgc
     3481 cgaagacccc gtacaggatt gccccggcgg ccgcggcgta taacccgttt tcaatcggaa
     3541 cgcgcgcgat ttcggcgtag ccgaggttct tgggaacgac gagcgcggtg accgtcacac
     3601 cggcgatgac gtccgaccgc agccaggacc ggtcgtactg acgcgcccag ccaatgatcg
     3661 gcaacaggcg gccgacacga ttgccccggg ttgctcgagc agattcattc acatccgcat
     3721 ggacatcgag gaccattcac gcactccccc ggcccaacct gcttactcaa agcgggttga
     3781 ggaggtaacc gaccgctgac cggcctgata caacgtccgg aaacaacgcc ccgcggacat
     3841 ggagaaaccg atggcaatgc gccaagcgag caagtccatg agtagcacga tgccaatagc
     3901 ttgtctagac aaatgtgctg tgcctggtat cggccacaga ccgcatcgcg ccgcccgtgg
     3961 gcaatcgttc gcttggcgta accaggcccg gcgcatgcgc gcgaaagtcc gcttcagtac
     4021 cgcatgggga cgtagcggta cgggtagtcc ggctcgacgt agccctcggc gggtcgtcgc
     4081 tcgggcaatg tgacgtccaa caccgggatc tcttcatacg gcaccagact cagcaggtgg
     4141 gaaatgcagt tgagtcgccc gcgccgcttt tcattggagt cgaccacgtg ccagggcgag
     4201 tcggccgtat cggtcgccgc gaacatcgca tcgcgggcgc gcgagtagtc gtaccaccgg
     4261 gcacaggact ccagatccat cgggctgagc ttccaggtct tgcgcccgtc cgagatgcgg
     4321 cgttcaaagc gccggagttg ctcctcctgg ctgacttcga gccagtattt gatcaggatg
     4381 atgccggaag cgatcatcgc ccgctcgaac gggatgacgt ggctcaggaa ttcatccacc
     4441 tgctgttcag tcgcaaaacc cattacgcgc tcaacgccgg cacggttgta ccaactgcgg
     4501 tcgaagatca cgacctcacc agcggctggg aagtgtggca catagcgctg aacgtacatt
     4561 tgcgacttct cacgctcagt gggtgcgggc agcgcgacga cggggaagac gcgcgggctg
     4621 actcgttccc gaatccgctt gatcgttccg cccttaccgg cggcatcgcg cccttcgaag
     4681 acgatgcaca ccttggcgcc ggtcgccttc acccacgcct ggagcttgac cagttctatt
     4741 tgaagcggct tgagcgcctt ttcgaattct cgagccgaca tccgtggctg ttccgactcc
     4801 ggattctccg ccccggcgct tgatgccatg accagcccat cgtcttggtg ctgatgcttg
     4861 tgcgtcttgt ctttcttgtg cttcacggtc gccatggtgc gggcctcctt caggaacctc
     4921 gtctctcgat gggatgacga gcggtatccc gttcggtcgg tggagaactc aaggtcagca
     4981 cttccgctgg aattgcggat gggtgggtct aatggggaga ctggatcgaa tacgcgccaa
     5041 tgcggcggcg tcctttccca agactccaga tggcttcgta tcgttcagta gttgagcata
     5101 cgaaccatgc gaaagatgtc aagcaaggcc gagcggccgg agcacgtcag cccccgctcg
     5161 gcatgcggaa gcaccgagac ctgaatccca ctcggccagc gagcaacagg tgaaggaaga
     5221 cccaatcgat ccgacccgaa cgagcacgtt cgctcgccag cgttctacga cgaaacagat
     5281 cgatttctac tgagtgttgg attcagtcac tagcccagaa ccaaccacca tccaccaacc
     5341 actaaccact cccattcgtc cactatcctt cccccatgac cgacatccgc accgtgaccg
     5401 ccacccgata cgtcgccccg cttcgggagg gcggctccct gcccggcctg atggaggccg
     5461 acgacctcgg cacctacgtc gtcaaatacc gcggcgccgc gca
//
LOCUS       DATAOZ010000629         5198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2876411, whole genome shotgun sequence.
ACCESSION   DATAOZ010000629 DATAOZ010000000
VERSION     DATAOZ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2876411"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VHR64_15650"
     CDS             complement(<1..435)
                     /locus_tag="VHR64_15650"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX3304319.1"
                     /translation="MTLVPWSSQGEPDPTPLDWLGKPDLSAPVRPTYWQIASIPRRGI
                     ACLIDMLILPAMMVAATYLIGERTTETIVRDGQLYFRIVAALSSWQARAVLLCWFGYL
                     IVTERLFGATLGKRITRITTIRGDGTPLTVKSAVLRWLPLLIP"
     gene            1031..1234
                     /locus_tag="VHR64_15655"
     CDS             1031..1234
                     /locus_tag="VHR64_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304320.1"
                     /translation="MSGCVLILVWIGCVLLGAAAGYAVGYLLWKLGFELIGSAVALVG
                     AGAGGILVFFGFLNWQEKRNPDM"
     gene            complement(1346..1759)
                     /locus_tag="VHR64_15660"
     CDS             complement(1346..1759)
                     /locus_tag="VHR64_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304321.1"
                     /translation="MIDLLLKILELLFADICGANGAAVASEIVQDSIRRVGLTQDKQG
                     RASRLQVIADLLAELVEIVARSGLEQLTHIASEGGAGGNPKHRNRKDQTRESSDQGTA
                     DGAVAHGMALAMVLDLSFLVLGHKGRIQDIEVARI"
     gene            complement(1818..3881)
                     /locus_tag="VHR64_15665"
     CDS             complement(1818..3881)
                     /locus_tag="VHR64_15665"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX3304322.1"
                     /translation="MRRFIRWFVGLAIVQALVLLLLAWLLPGFSLGEIQRAIPSALLI
                     SGLLALAWPFIYLIAGRFHPALFPVLTFLLTGFVIYLVGTVQIEGFRVDSIWTGIAVS
                     VGLTIADVMVGSLFSIDDSLAYDWFVVRPLKRKFDSTPSQTTPGILFLEIDGLAEPIL
                     RQAIDSGYMPTLKRWIDEGSHRLMSWEPDLSSQTSASQAGILLGDNTGIPAFRWYDKE
                     AGKLMVSSNMEAAKALERRLSTNKGLLTDGGASRWNVFSGDASDCLCTFSAFNDRERS
                     TSRSYAAYFSNPFTFARTVGLLVGDVARERYQAWRQVHNDVRPRIHRRFKYAFVRAGT
                     TTVMQEASLFMLITDMFRGVPAVYNTFFAYDEVAHHSGIDRPDAFKILRTLDQVFAKL
                     ERAARVARRPYRFVVLSDHGQSMGATFRQRYGQTLSELVSSLISNEHKIASAEAAVED
                     WGNLNLAVNAAIQQDTRTARLIHRVVRGQMDDGVVTLGPDDEPSDSDRQEQVDAANVI
                     VLASGNLGLISFPDWKSRMTLEQISSSFAGLVNGLASHDGIGFVMVHSESDGAVVIGR
                     SGIHYLDDDHIVGDDPLSGFGRNAARHLKREDSFVDAPDILVMSLHNELTGEVAAFEE
                     LVGCHGGLGGPQTQPFVLFPAEFDAPEEPIIGAAALHDVLKSWRIRAAEGAAPTAAQV
                     ATVVS"
     gene            4242..5039
                     /locus_tag="VHR64_15670"
     CDS             4242..5039
                     /locus_tag="VHR64_15670"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX3304323.1"
                     /translation="MSEQRATPTTFDVFVLALSAFSLINIIWILILQNGHVRNVVLIV
                     DPICSVVFLADFFLQLHRAPTKSSYFVKHWGWLDLVGSFPFPLFRIARIIRVFRIYQP
                     LKRTGGKGVIRQANADRAGSALLIAIFLTIIVIQYASMSILWAEGKNAHGNIHTASDA
                     VWWSYVTITTVGYGDRFPVTNLGRLVGVALLSSGVGLFAVITGFLANSFLSRRGPREP
                     TVTPEQLDARLRRLEALGDAIRVASVDGTTPAAVEPSATESPEGMAE"
BASE COUNT         1067 a         1564 c         1544 g         1023 t
ORIGIN      
        1 tgggatcaaa agcggcagcc accggagtac cgcggacttc actgtcaggg gcgtcccgtc
       61 gcccctgatg gtggtaatcc gggttattcg cttgccgaga gttgcgccga agagtcgctc
      121 ggtgacgatg agatagccga accagcacag aagcacggcc cgagcttgcc acgagctgag
      181 cgccgcaacg atccggaaat acagttgccc atccctgacg atcgtctcgg ttgtgcgctc
      241 tccgatcagg tacgtcgctg cgaccatcat ggccggcaga atgagcatgt cgatcaggca
      301 cgctatgccc cgccggggaa tactggcgat ctgccaatac gttggtctga ccggggcgga
      361 gagatccggc ttcccaagcc agtcaagcgg agtaggatct ggttcccctt gggaagacca
      421 tggaacgagg gtcatggaca cgacgaaacc tccccggaag gactccatcc ccaccaatca
      481 ggacgccgag ttcagccgac ctaccctgcc gacggccagg caacgccacg gttaccgacc
      541 attcagcatg accgactgtt agcaacgtga gcttttcggc tcgtaggaca tcgaatctct
      601 caggtcgtcg tcgacctgtc ccggttgacc tgctgttggt atcactcgcc gccttcagcg
      661 ccaacgaact cacagctgag ctgtcaaaag cgaggacgca cctacggcac agcaacgtcc
      721 ggaacatgcc cagtcgcgca ggtcaaggaa ccgcattgcg aatacgccaa agggatgatg
      781 cgagtcctcc caatcgcttg gcaagatggc cgagttctag caatggcctg cccggcgctg
      841 acgactgcgc gcatcgcgac gtccagagga tcagggtcga ctcatttcct gtcgcgatct
      901 ctgcgcgctt gatcgtctct ggcagccatt cgatgcaggt gacgtgcgca atcagttgga
      961 atgagatcga cgttgccagt atgttgcccg gttggattcc ggctgcggcc gacgacggac
     1021 ggaggtgagc atgagtggct gcgtgctgat attggtctgg atcggctgcg tccttctggg
     1081 cgcggcagcc ggctacgcgg tcggctatct cctatggaag ctcggcttcg agctgatcgg
     1141 cagcgccgtt gcgctcgtcg gcgcgggagc cgggggaatc ctggtcttct tcggattcct
     1201 caattggcag gagaaacgca atcccgacat gtgactcact gcgacgcgag tcgattgaga
     1261 ggagttgatt atggcgacgc tgtacgcaat cgtttatccc gatgcggcca ccgccgagca
     1321 agctgagcag accatgcggg gcctctcaga tgcgggctac ctcgatatcc tggattcgtc
     1381 ccttgtgacc aagaaccagg aaggaaagat cgagcaccat ggcgagcgcc attccgtgcg
     1441 ccacggcgcc gtcggcggtg ccctgatcgg aggattcacg ggtctgatct ttgcggttcc
     1501 ggtgcttggg attgccgccg gcaccgcctt cggaagctat gtgggtaagc tgctcaagtc
     1561 cggagcgagc aacgatttca acaagttccg cgagcaggtc agcaatgact tgcagccggg
     1621 aggcgcggcc ctgcttgtcc tgggtcagac cgacgcgccg gatcgaatct tgcacgatct
     1681 cggacgccac ggcggcaccg ttcgctccac agatatcagc gaaaagcaac tcaaggatct
     1741 tcagcagcag gtcgatcacc tgtcctcaac atctgcaccg ccagaatctg cacagtcaga
     1801 catgtcgacc cactgagtca cgagacgaca gtcgcgacct gggctgccgt gggagctgct
     1861 ccctcggcgg cccgaattcg ccatgacttg agcacgtcgt gcagtgcggc ggctccaatg
     1921 attggttcct ccggagcgtc aaactcggcc gggaagagca cgaagggctg tgtttgcggt
     1981 ccgcccaatc cgccgtggca tccaaccagc tcctcgaacg cggccacttc accagttaat
     2041 tcattgtgaa ggctcataac gaggatatct ggagcgtcga caaagctgtc ctcccgtttc
     2101 aggtgtcgcg ccgcgttcct cccgaaaccg gacaaggggt catccccgac gatatggtcg
     2161 tcatcgagat aatggatgcc gctccttccg atgaccaccg caccatcgga ctcggaatgc
     2221 accatcacga agccgatccc atcatggctc gccaatccat tcaccagccc ggcgaaactg
     2281 gacgagatct gctccagcgt catccgcgat ttccagtcgg gaaatgagat gagcccgaga
     2341 ttgccggagg caagaacgat gacgttcgcg gcatcgactt gctcctgtcg atcgctgtcg
     2401 gatggctcgt cgtcaggacc gagggtgacg acgccatcgt ccatctggcc gcgcacgacc
     2461 cggtgaatca atcgggcagt tcgcgtgtcc tgttgaattg ccgcgttcac ggcaagattg
     2521 agattgcccc agtcctcgac cgcggcctcg gcgctcgcaa tcttgtgctc gttcgagatc
     2581 agcgagctga ccaactcaga aagggtctgc ccgtatcgct gccggaacgt cgcgcccatg
     2641 ctctggccat ggtcggaaag cacaacgaac cggtatggcc ggcgtgcgac cctggcggcg
     2701 cgttccagct tggcgaatac ctgatccaac gtgcgcagga tcttgaatgc gtcgggccga
     2761 tcaattccgg agtggtgggc aacctcgtca tacgcaaaga acgtgttgta gacggctggg
     2821 accccccgaa acatgtccgt gatcaacatg aacaggctgg cttcctgcat gacggtcgtg
     2881 gtgcccgctc gcacgaacgc gtacttgaat cggcggtgga tccgcggtcg gacgtcgttg
     2941 tgaacctggc gccaggcctg gtaccgctcg cgagcaacgt cgccaaccag cagtccaacc
     3001 gtgcgcgcga aggtgaacgg gttgctgaaa tatgcggcgt agcttcgcga tgttgatcgt
     3061 tcgcggtcgt tgaatgcgct gaatgtgcaa agacaatcgg atgcgtcacc ggaaaagaca
     3121 ttccaccggc tggcgccacc atctgtgagc agacctttat tcgtagaaag acgccgctcc
     3181 agcgccttgg ccgcttccat gttgctggac accatcaact tcccggcctc cttgtcgtac
     3241 cagcgaaacg ccggaatccc ggtgttgtct ccaagcagga ttccggcctg gctggccgac
     3301 gtctgtgacg acaaatccgg ctcccaggac atcagacggt gcgatccctc atcgatccag
     3361 cgtttgagcg tcggcatgta cccgctgtcg attgcctggc gcaggatcgg ctcggccagg
     3421 ccgtcaatct ccaggaacag aattccgggg gtcgtctggc tcggggtcga gtcgaacttc
     3481 cgtttcagcg gacgcacgac aaaccagtcg taggcgagtg aatcgtcgat tgagaagagc
     3541 gaccccacca tcacgtccgc gatcgtcagg ccgacgctga cggcaattcc ggtccagatg
     3601 ctatcgacgc ggaacccctc gatctgaacg gtgccgacga ggtagatcac gaatcctgtg
     3661 agcaggaatg tcaggaccgg gaagagcgcg ggatggaagc gaccggcaat caaataaata
     3721 aacggccaag caagcgcgag cagaccggaa atgaggagcg cgctcggaat tgcccgctgg
     3781 atttcaccaa gtgaaaaccc gggcaagagc caggcaagta ggagcaggac caatgcctgt
     3841 acgattgcca ggcccacgaa ccagcgaatg aaccgtcgca tcgacgcacc tcgttgagcg
     3901 aatcgtcgat ttctgtgttc ggtcgcacgt aaagactggc gcaagccggg tggtcggaaa
     3961 aactccgtgc ccacagtgat caaacgatct cagccgcgct cagccgtcgt cacatcatgg
     4021 ggcaaacagg gcctaatcgc atcggtcgaa ggactcatat aggggcaatt catgagtcaa
     4081 cggcagaatt caggagcggg aatgatccct tcgatggatg cgactacgac cacaccgggc
     4141 atacgctgct cccgcctgga tgtcccgaac tgcgggcaca cgcgcgtggt catgcccggt
     4201 aaccgatccg gggtgagcga tcacatcgtg agatgtttga gatgtcggaa cagcgcgcga
     4261 cgccgacgac attcgatgtg tttgttttgg cgttgtctgc attctcgcta atcaacatca
     4321 tctggattct gatcctccag aacggtcatg tccgcaatgt cgtcctgatc gtcgatccga
     4381 tctgctcggt tgttttcctt gccgatttct ttctgcagtt acatcgggca ccgacgaagt
     4441 cgtcctactt cgtcaagcat tggggatggc tggatttggt cggcagcttt ccgtttcccc
     4501 tttttcgcat cgcgcgaatc attcgggtgt tccgcatata tcaaccgctc aagcggaccg
     4561 gcggtaaagg cgtcattcgc caggccaacg ccgatcgagc tggcagcgcc ctgctgatcg
     4621 cgatcttcct cacgatcatc gtgatccagt acgcgagcat gtcaattctc tgggcggaag
     4681 gtaagaacgc gcacgggaac atccacaccg cgtcggacgc cgtctggtgg tcatacgtga
     4741 cgatcacgac ggttggctat ggcgaccgat tccccgtcac gaatctgggc agactggtcg
     4801 gcgttgcgct gttgtccagt ggagttggtt tgtttgcggt catcaccggc tttctcgcca
     4861 attcgtttct gtcgcgccgc ggaccgcgtg aaccgaccgt cacgccggag cagctggacg
     4921 cccggctgcg gcgactcgaa gccctgggag acgcgattcg cgttgcttcg gtagacggaa
     4981 cgactcccgc ggcggttgag ccttccgcca cggaatcacc ggagggtatg gccgaatagc
     5041 caagactcgt tgatgtcgcc ggcaggcccc ttgtcggtgg aacctgacta atttgcgcga
     5101 atactccatt cgattgacga cgctacgacc acaccttcct agtatgaagt tgcgtccaga
     5161 cgacagacac cagcctcaga accgttgcac ataccctg
//
LOCUS       DATAOZ010000630         7695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8926370, whole genome shotgun sequence.
ACCESSION   DATAOZ010000630 DATAOZ010000000
VERSION     DATAOZ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8926370"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..2405)
                     /locus_tag="VHR64_15675"
     CDS             complement(147..2405)
                     /locus_tag="VHR64_15675"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX3304324.1"
                     /translation="MSFLSPARLARASARHPWRVLGAWLVVLVLAVMSASSLGDALTT
                     TSNFSGKPDSAVGEQLLKDRMRGEKPATETIIVRSETLTVDDPGFKAIVEKTSSDVLA
                     IPGAITSAPTYYQAVAAGDTGAANMVSADRHATIIPVTFAGKFNDADKYADDYLASVK
                     QHATADVQVLTVGDVSVNDAFNTISSHDLEKAEMFSLPLTILILVVVFGALVAVGVPL
                     VLAITSIILALGLTAVVGRFTDLSFYVVNMITMIGLAVGIDYTLFVVSRYREERQRGL
                     AKLDAIETAGGTATKAVVFSGGTVVLALMGMFLVPLTVFHSLGAGAVLAAVSAVAGTL
                     TLVPAMLGLLGDKIDWPRKRRYDDAAVRAANQAHANETYHAGFWGRVTRVVMARPAVF
                     TVLSIGLLVTLALPYFDIHRGQAGVETLPASDVKTAYEVLGKDFSVGLASPVEIVVDG
                     DTSKLGVQASIAALQTALDEDQNFGPSTVQINQSGDLALISAPLNLDAESEAAVAAIK
                     NLRDNTIPQAFSSSADTSVYVSGGPAFNTDYFHIVDSSTPTVFGFVLGLSFVLLLLAF
                     RSIVIAAKAIVMNLLSVGAAYGTLVLVFQKGYGNELFGFQQSPTIEAWIPIFLFCVLF
                     GLSMDYHVFLLSRIREHFDLTRRNTESVAVGLQSTARIITGAALIMVIVFGGFATGEL
                     VAFQQMGFGMAVAVFLDATVVRTILVPAAMALLGDRNWYLPKWLSWLPNVGVEGAPVL
                     APAPALTTSAAD"
     gene            3024..4481
                     /gene="ngcE"
                     /locus_tag="VHR64_15680"
     CDS             3024..4481
                     /gene="ngcE"
                     /locus_tag="VHR64_15680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_function: GO:0048030 - disaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0015764 - N-acetylglucosamine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine/diacetylchitobiose ABC
                     transporter substrate-binding protein"
                     /protein_id="HEX3304325.1"
                     /translation="MSTQDQIQKLLVDAQSGQHSRRDLLRRAAAMGLSFPAAVALFTG
                     AMPLSALAQDASPAAGATNPLGVDANAPLDVVIFKGGFGDNYAINVNQNLYNKLYPGA
                     QITYAGIQGLGAQLQPRFVDGSPPDVIDNSGAGNLDTAALVAEGQLADLADLMAAPSY
                     DTEGKTFAETLVPGSQVDGVFDGKQLYLNWALTVVGVWYSSTWMESKGYTYPKTWSEF
                     VALCEEIKGAGVAPFGTTGVHPQYIKNFMFDQMLYKHDPDAAIAIDNLEPDAWKSQAV
                     TDVLTALYQLAEKDLFLPGWEALDHTQSQAEWLQGKAAFLPCGTWLQNEMGDLVPPDF
                     NMVVAPTPSLDGDKLPFEAIIATAGEPFIVPAQGKNVAGGKEWLRLLFSKEGGRFFAE
                     DTKSLSVVLGSGEGLDLGTAFQSTQEAIAAAGSNGFPTARYQSWYKDLQDEARFQLLA
                     LMQKQISIEEYQSTVQDMADQVKDDDSVVKYTREK"
     gene            4686..5579
                     /locus_tag="VHR64_15685"
     CDS             4686..5579
                     /locus_tag="VHR64_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3304326.1"
                     /translation="MHHHKYRLIIPFLLPALLLYGVFVLWPYAQAIYVSFTSWRGVSP
                     DKPWVGLDNYQRAWNDTRLIDALSRNAQILIVLPLVTITIALTFAALFTQGSNAIRGA
                     GFYRVVFFFPQVISAVIVGMLWSYVYHPNVGLLNGVLGSVGLGSLERAWLGNPTTILW
                     AIVAVAVWSSVGFYMVIYIASMQSIPTSFYEAAVLDGANRWTSFRDITFPLIWETLRT
                     TLIYIGIIALDLFVLVQVMTGGGVGSGRRAEVAALYLYNQAFDRSRWGYASAIGVILL
                     VLTLLLSVVIMRLTRRETYEF"
     gene            5593..6504
                     /locus_tag="VHR64_15690"
     CDS             5593..6504
                     /locus_tag="VHR64_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3304327.1"
                     /translation="MIDDILQPIAEPVPARGKLHRIAGKVGLGNIAFNGISQVLLGFW
                     ALMVIFPFLWMIMTSFKTDPEILFSPWKFPDTLQWNNFSRAWNEAHIGRYFLNSLIVI
                     IPSLTGTLLVSAMTAYVLARFEFPGRRILYYLFMMGLMFPVFLALVPLFFLVQDLHMT
                     ATYQGLILVYIAYSLPFTVFFLTGFFRTLPSEVGEAALVDGASHYRIFFNIMLPMAAP
                     GLIAMGIFNFLGQWNQFLLPLVLMPDQNKYVLSQGLAFLAIQQGYANDYSALFAAMTI
                     TMIPTLIVYVLFQRRLESGLTAGALKG"
     gene            6647..7501
                     /locus_tag="VHR64_15695"
     CDS             6647..7501
                     /locus_tag="VHR64_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase subunit LigB"
                     /protein_id="HEX3304328.1"
                     /translation="MAPHGWTLIPELADEPDDCHVTRAAMEELGRRIEAAKPDVVVLA
                     TPHGFRVRDAIAFADVARMAGTLHHNGKTVEMNVPVDRQLAAEIAKTANSRGIPVAMA
                     GFAGNSDSESAIPLDWGTFVPLWFMGHGRNMTGYGHVLADKPEQEQGPPVVVMNPARA
                     LPWETNIRLGEAIADTAMRDGRRIAFVASCDWAHAHEGSRYGASPAAAEVDGAVEAAL
                     RDGDPGRLIDLDPEKVSKAAVDGLWQALILAGVMNRVPMQGEVLSYEVVRAYSVGMLV
                     ATYEPTTA"
BASE COUNT         1384 a         2377 c         2531 g         1403 t
ORIGIN      
        1 cgatcaacac cctgtgagcg cccgtctcga ccgttccgat ccgcgcttcg ttcgtcgatt
       61 gggtggagca agtcgtcagg gccaaaccaa agcgccggac gggatgtccc gtccagcgct
      121 ttggtgagag aggagtggat ggcgtgttag tcggcggctg aggttgtcag ggccggagcg
      181 ggggcgagca ccggggcacc ctccacgccc acgttcggca gccagctcag ccacttcggc
      241 aggtaccagt tgcgatcacc gagcagggcc atcgccgccg gcaccaggat cgtgcggacc
      301 accgttgcgt ccaggaagac cgcgaccgcc atgccgaagc ccatctgctg gaaggcgacc
      361 agctcgcccg tcgcgaagcc accgaacacg atcaccatga tcagggcggc gccggtgatg
      421 atccgcgcgg tcgactggag gcccaccgcg accgactcgg tgttgcgccg ggtcaggtcg
      481 aagtgctccc ggatccggct cagcaggaag acgtggtagt ccatcgagag gccgaacagg
      541 acacagaaga ggaagatcgg gatccaggct tcgatcgtcg gcgactgctg gaagccgaac
      601 agctcgttgc cgtagccctt ctggaagacc aggaccagcg tgccgtaggc cgcgcccacc
      661 gagagcaggt tcatcacgat cgccttggcg gcaatcacga tcgagcggaa ggccaggagc
      721 aggagcacga agctcaaccc gagcacgaat ccgaacaccg tcggcgtcga gctgtccacg
      781 atgtggaagt aatcggtgtt gaaggccgga ccgccgctga cgtagacgga ggtatcggca
      841 ctgctcgaaa aggcctgcgg aatcgtgttg tcgcgcaggt tcttgatcgc ggccaccgcg
      901 gcctcggact cggcgtcgag gttgagtggc gccgagatca gggccaggtc gccggattga
      961 ttgatctgca ccgtgctcgg accgaagttc tggtcctcgt caagcgccgt ctgcagggcc
     1021 gcgatcgagg cctggacacc cagcttgctg gtgtcgccgt cgaccacgat ctcgaccggc
     1081 gaggcgaggc cgacggagaa gtccttgccc aggacctcgt aggccgtctt gacgtcgctg
     1141 gccggcagcg tctcgacgcc ggcctgtccg cgatggatat cgaagtaagg cagggcgagg
     1201 gtgacgagca ggccgattga cagcacggtg aagacagccg ggcgagccat cacgacgcga
     1261 gtgacccgac cccagaaacc ggcgtggtag gtctcgttcg catgcgcctg gttggcggcg
     1321 cggacggcgg catcgtcata gcggcgcttg cgcggccagt cgatcttgtc gccgaggagg
     1381 ccgagcatag ccggaaccag cgtcagcgta ccggcgaccg ccgagacagc tgccagcacc
     1441 gcaccggcgc cgaggctgtg gaagacggtc aacgggacca ggaacatgcc catcagcgcg
     1501 aggacgaccg tgccgccgga gaagacgact gccttcgtcg cggtgccgcc ggcggtctcg
     1561 atcgcatcga gcttggccag gccgcgctgc cgctcctcgc gataccgcga gacgacgaag
     1621 agggtgtagt cgataccgac cgcgaggccg atcatcgtga tcatgttgac gacgtagaag
     1681 gacagatcgg tgaaccggcc gaccacggcg gtcagaccga gcgccaggat gatcgaggtg
     1741 atcgcaagca ccagcggcac gccgaccgcg accagggcgc cgaagacgac gaccaggatc
     1801 aggatcgtca gcggcaggct gaacatctcg gccttctcga ggtcgtgcga tgagatcgtg
     1861 ttgaacgcat cgttgacgct cacgtcaccg accgtcagga cctggacatc ggcggtggca
     1921 tgctgcttga ccgacgcaag gtagtcatcg gcgtacttgt cggcatcatt gaatttgccc
     1981 gcgaaggtga ccgggatgat cgtcgcgtgg cgatcggccg agaccatatt ggcggcgccc
     2041 gtgtcaccgg cggcgacggc ctggtagtaa gtcggagcgg aggtgatcgc gcccgggatc
     2101 gcgaggacgt cggaggacgt cttctcgacg atcgccttga agcccggatc gtcgaccgtc
     2161 agcgtttccg agcggacgat gatcgtctcg gtggcgggct tttcgccgcg catgcggtcc
     2221 ttgagcagct gctcgccgac ggccgaatcc ggcttgccgg agaagttgct ggtcgtggtg
     2281 agcgcgtctc caaggctgga ggcggacatc accgcaagga cgagaacgac gagccaggcg
     2341 ccgagaacgc gccatggatg gcgagcgctt gcccgggcta gccgggccgg cgatagaaac
     2401 gacatggtgc tctctccttt ggtgtctgcg tttccgatca gatcaactga ctcgattctg
     2461 ggtttcgggc gaatcggtgt cgtcggacaa gtagacgaat cagggcgcgg acctgtgacg
     2521 gacagcacgc gtggaccgat ggccgacggc gcatctccgc caaatgacgg agggcggaag
     2581 caggtacggt tttgagccag agtgattgag aacggggatc tttgccgggc ccgactgccg
     2641 cccaaggctg ggcctgaatg cgcgccacaa acgatgttgt tgcccgctac aactggtccg
     2701 catttggtat acgaattcgc atattcgtat cggcgtgcgc tagagttacc gccacgtcgg
     2761 tttgctgccc tcattgggcg cggttacgtt gcatattcgg ttcgtcccgc gatgcgttca
     2821 gcgtggagtc ccttccggga cgactggacc gtcgcgtcgg gatcttcaag aggaggatgg
     2881 ctaccggaga cgcggacaac tgaatatgtc cttgtgatgc ccacaacgat tgtcaacgat
     2941 gcacctggtg gggccgtcac gcttgtttgt tggcgcctga ccgaatcaga gagtgtggtc
     3001 agcgcccaat ttggagggga tcagtgtcga cccaggatca gattcagaag cttcttgtcg
     3061 atgcccagag tggccagcac tcccgccgcg atcttctgcg ccgggccgct gcgatgggtc
     3121 tttcgttccc ggcagccgtc gcgctgttca ccggcgccat gccgctgagc gcgctggccc
     3181 aggatgcctc cccggccgcc ggcgcgacca acccgctcgg ggttgatgcg aacgcgccgc
     3241 tcgatgtcgt catcttcaag ggtggcttcg gcgacaacta cgcgatcaac gttaaccaga
     3301 acctgtacaa caagctctac ccgggcgccc agatcaccta tgcgggcatc cagggcctcg
     3361 gcgcgcagct tcaaccgcgc ttcgtcgacg gcagcccgcc ggacgtgatc gacaactcgg
     3421 gcgccggcaa cctcgatacg gccgcgctgg tggctgaggg tcagctggcc gatctggccg
     3481 acctgatggc ggcgccatcc tacgacaccg agggcaagac ctttgccgag accctggtgc
     3541 cgggctcgca ggtcgacggc gtattcgatg gcaagcagct ctatctgaac tgggcgctga
     3601 cggtcgtcgg tgtctggtac agcagcacct ggatggagtc caagggctac acctacccga
     3661 agacctggtc cgagttcgtc gcgctctgcg aggagatcaa gggcgcgggc gtggcgccgt
     3721 tcgggacgac cggcgtacac ccgcagtaca tcaagaactt catgttcgat cagatgctgt
     3781 acaagcacga tccggatgcc gcgatcgcga tcgacaacct cgagccggat gcctggaagt
     3841 cgcaggccgt gaccgacgtg ctgacggcgc tctatcaact ggcggaaaag gatctcttcc
     3901 tgccgggttg ggaagcgctc gatcacaccc agtcgcaggc tgagtggctc cagggcaagg
     3961 cggcattcct gccgtgcggc acctggctgc agaacgagat gggcgatctg gttccaccgg
     4021 atttcaacat ggtcgtcgca ccgacgccgt cgctcgatgg cgacaagctg ccgttcgagg
     4081 cgatcatcgc cacggccggc gagccgttca ttgtcccggc ccagggcaag aacgtcgcgg
     4141 gcggcaagga gtggttgcgc ctcctcttct cgaaggaggg cggtcgcttc ttcgccgaag
     4201 acaccaagtc cctctcggtc gtacttggct ccggcgaagg actcgatctg ggcactgcct
     4261 tccagtcaac acaggaagcc attgccgccg ccggctcgaa tggcttcccc acggcgcggt
     4321 accagtcctg gtacaaggac ctccaggatg aggctcggtt ccagctgctg gcgctgatgc
     4381 agaagcagat ctcgatcgag gagtaccaga gcaccgtcca ggacatggcc gaccaggtca
     4441 aggacgacga ctcggtcgtg aagtacaccc gggagaagta actcccgttg tgtctggccc
     4501 gatctgccgg ggatccctcc ggcagatcgg gcattcccga attcggcaat accgctccgt
     4561 ttgcgctcgt cacgagcttg tcgagcgccg tatcattttc gccacacgaa cacgcaatca
     4621 ccaaaacagg acataagaca agaaccggtt cacggtgccg tggctggggg aatcgaagac
     4681 gttccatgca ccatcacaag tacaggctga tcattccctt cctgctgccg gcgctgctgc
     4741 tgtacggcgt ctttgtccta tggccctacg cccaggccat ctacgtgtcg ttcacgagct
     4801 ggcgcggcgt ctcccccgac aagccgtggg ttggactcga caactaccag cgggcctgga
     4861 atgacacccg actgatcgac gcgctctccc gtaacgccca gatcctgatc gtgctgccgt
     4921 tggtcacgat cacgatcgcg ctgaccttcg cggcactttt cacgcagggc agcaatgcca
     4981 tccgcggcgc cggcttctac cgcgtcgtct tcttctttcc gcaggtcatc tcggcggtca
     5041 tcgtcggcat gctctggagc tacgtctacc acccgaatgt cggcctgctg aacggtgtgc
     5101 tcggctccgt cggactaggc agcctggagc gcgcctggtt gggcaaccca accacgatcc
     5161 tgtgggcgat cgtggcggtg gcggtctggt ccagcgtcgg cttttacatg gtgatctaca
     5221 tcgcgagcat gcagtcgatt ccgacgtcgt tttacgaggc ggcggtgctc gacggcgcga
     5281 accggtggac ctcgttcagg gacatcacct tccccctgat ctgggaaacg ctgcgcacga
     5341 ccctgatcta catcggcatc atcgcgctgg acttgttcgt actggtccag gtgatgaccg
     5401 gcggtggcgt cggctccggg cggcgagccg aggtggccgc gctctatctg tacaaccagg
     5461 cattcgaccg gagccggtgg ggctacgcct cggcgatcgg tgtgatcctg ctcgtcctta
     5521 cactgctgct ctcggtcgtc atcatgcgat taacgcgtcg cgagacctac gagttctaga
     5581 ggagcgaccc gcatgatcga cgacatcctt caacccatcg ccgagccggt accagccagg
     5641 ggcaagttgc atcgcatcgc cgggaaggtc ggcctcggca acatcgcgtt caacggcatc
     5701 tcccaggtgc tgctcggctt ctgggcgctg atggtgatct tcccgttcct gtggatgatt
     5761 atgacctcgt tcaagacgga cccggagatc ctgttcagcc cctggaaatt ccccgatacg
     5821 ctccagtgga acaacttctc ccgtgcctgg aatgaggccc acatcggccg ctacttcctg
     5881 aactcgctta tcgtgatcat cccgtcgctg accggtacgc ttctggtctc ggccatgact
     5941 gcctatgtgt tggcccggtt cgagttcccg ggtcggcgca tcctctacta cctgttcatg
     6001 atggggctga tgttcccggt tttcctggcg ctggtgccgc tcttcttcct ggtccaggac
     6061 ctgcatatga ctgcgaccta ccaggggttg atcctggtct acatcgccta ttcgctcccc
     6121 ttcactgtct tcttcctgac cggtttcttc cggacgctgc cgagtgaagt cggagaggcg
     6181 gcgctggtcg acggagcgag ccattaccgg atctttttca acatcatgct gccgatggcc
     6241 gcgccgggcc tgatcgcgat gggcatcttc aacttcctcg gccagtggaa ccagttcctg
     6301 ttgccactgg tgttaatgcc ggaccagaac aagtacgtgc tgtcgcaggg gctcgccttc
     6361 ctggcgatcc agcagggcta cgccaacgac tacagcgcgc tcttcgcggc gatgacgatc
     6421 acgatgatcc cgacgctgat tgtctacgtc ctgttccagc gccgcctcga gtcgggcctg
     6481 acggccggag cgctgaaggg gtagagaggt tttaggttct gggtcttggg ttttaggcgc
     6541 ttgatagcgc atggtaggta gagaaatgaa agatcccgaa aaccgatcat cccaaaaccc
     6601 aaaacctgaa acccaaaccc caaacggtgg gatcgtcttt gcgtgcgtgg cgccgcatgg
     6661 gtggacgctg attccggagc tggctgatga gccggatgac tgccacgtca cccgggcggc
     6721 catggaggaa cttgggcgcc ggatcgaggc ggcgaagccg gatgtggtcg tgctggccac
     6781 tccgcacggg ttccgggtcc gcgacgcgat cgcatttgcc gatgtcgccc ggatggccgg
     6841 cacgctccat cacaacggca aaaccgttga gatgaatgtg ccggtcgacc ggcaactggc
     6901 tgccgagatc gccaagaccg ccaactcccg ggggatcccg gtcgcgatgg ccggttttgc
     6961 cgggaacagc gactccgaga gcgcgattcc cctcgactgg ggaacgttcg tgccgctctg
     7021 gttcatgggc cacggccgga acatgaccgg ctacgggcac gtcctggccg ataagccgga
     7081 gcaggagcag ggtccgccgg tggtggtgat gaacccggcg cgtgcgctgc cctgggagac
     7141 gaacatccgg ctgggcgagg cgatcgccga taccgcgatg cgcgacggtc gccggatcgc
     7201 cttcgtcgcc tcgtgcgact gggcgcatgc gcacgagggg agccgctacg gcgcgagccc
     7261 ggccgcggct gaggtcgatg gggctgtcga agccgcactg cgggacggcg accccggtcg
     7321 actgatcgat ctcgatccgg aaaaggtctc gaaggccgcg gtcgacggac tctggcaggc
     7381 gttgatcctg gccggggtga tgaaccgggt accgatgcag ggcgaggtgc ttagctacga
     7441 ggtcgtccgc gcctattcgg tcgggatgct cgttgcgacc tacgagccga caacagcctg
     7501 atattaagaa ggaacagcac acgtgggtga tttgcagggg aagattgcgc ttgtcaccgg
     7561 gagcacccgg ggcctggggc ggaccgccgc cgagtggctg gcgaaggacg gcgcgtcggt
     7621 aatcgtctcg gggcgcgagg tcgaggcggt cgaggagtcg gtccggtcga tcgaggcgct
     7681 cggcgtgcag gcgtg
//
LOCUS       DATAOZ010000631         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6561102, whole genome shotgun sequence.
ACCESSION   DATAOZ010000631 DATAOZ010000000
VERSION     DATAOZ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6561102"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VHR64_15700"
     CDS             <1..412
                     /locus_tag="VHR64_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304329.1"
                     /translation="KIIDIDLERRRISLSLKQANEGVDATADDFDPTLYGMAASYDDQ
                     GNYLYPDGFDPETGEWLEGYEEQRAAWEQQYAEAHARWEAHKKQVEDADGAAAPASSS
                     YSSDAGSSPTPPAEGSLASDEALQALRQKLTGGS"
     gene            586..1185
                     /gene="coaE"
                     /locus_tag="VHR64_15705"
     CDS             586..1185
                     /gene="coaE"
                     /locus_tag="VHR64_15705"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX3304330.1"
                     /translation="MLRVALTGGIASGKSLVAAELGARGAVIIDADVLAREVVEPGTP
                     ALAAIIDKFGPQIMRDGELDRSQLGAIVFADPDARRDLERIVHPAVRARAAELERAAD
                     PDAVVVHVIPLLVETGQQEGFDVVVTVDVDHETQIQRLIARNGFGRAEAAARVDAQAD
                     SEERRTAADVVVDNHGNLEDLREQIAALWDVLTSAATRE"
     gene            complement(1348..1488)
                     /locus_tag="VHR64_15710"
     CDS             complement(1348..1488)
                     /locus_tag="VHR64_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304331.1"
                     /translation="MVKAIRYVCGRWPAPRGMIDEINANEATGSSGRAAKDFRRAKSA
                     ER"
     gene            1481..>2207
                     /locus_tag="VHR64_15715"
     CDS             1481..>2207
                     /locus_tag="VHR64_15715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX3304332.1"
                     /translation="MTIALIVALIAAIFGAMPIAVLVAAFAVPVVYIVYIYDVNLWED
                     EPLIVTGLAFLLTGALTVGFTILWTYLRGPVPYGTTTYEGSLSAAPTVGAFLLVALAV
                     PIVGEVIRQIGPVLLASRPEFDDLMDGLTFGVISGLAYSCFDTLTRHWDLLTGGLQGP
                     EPGLWVSLIFLEGFVKPMIIGTASGIAVAEFSGLGRGYDGFTPRYFRGVGEAILANAA
                     YQAGTYLFSFVGNATLGVVLSILW"
BASE COUNT          369 a          686 c          732 g          420 t
ORIGIN      
        1 caagatcatc gacattgatt tggagcgccg ccggatctcg ctctcgctga agcaggcgaa
       61 tgaaggcgtc gacgcgactg ccgacgactt tgatccgacg ttgtacggca tggccgcctc
      121 ctacgacgac cagggcaatt acctgtatcc ggacggattc gatccggaaa ctggcgagtg
      181 gctggaaggt tacgaagagc agcgcgcggc ctgggagcag cagtatgccg aggcgcatgc
      241 tcgttgggag gcgcacaaga agcaggtcga ggacgccgat ggcgccgccg cacctgcctc
      301 gtcgagctac tcctccgacg ccggatcctc tccgacgccc ccagccgaag gctcactagc
      361 ctccgacgag gcgcttcagg cgttgcgtca gaagctcacc ggcggtagct gatcaggatg
      421 aaatgcgggc gagcgtcggg tagggagggc gacgggtcct gtctggacgg ccaagcgagc
      481 acgacctcat tgcgaccgat ctaggttgcg cttggcgttg tcgtgcgagc gcgggaggaa
      541 gacaggatct gtggagcccg accgggcgcg agcccgcaac aaaaaatgct gcgggtagcc
      601 ctgacgggcg gcatcgcgtc gggcaaaagc ctggttgccg ctgagttggg ggcgcgtgga
      661 gcggtgatca tcgacgccga cgtgctcgcc cgcgaggtgg tggagccggg tacgccggcg
      721 cttgccgcaa ttatcgacaa gttcggtccg cagataatgc gcgacggaga actcgaccgg
      781 tcacaactcg gcgccatcgt cttcgccgac ccggatgccc gccgcgacct cgagcggatc
      841 gttcatcctg ccgtacgagc ccgagcggca gagctggagc gcgccgccga tcccgacgca
      901 gttgtagtcc atgtcattcc gctgctcgtc gagaccggcc agcaggaggg tttcgacgta
      961 gtggtcacgg tcgatgttga tcatgaaacc cagatccagc ggctaatcgc gcgcaacgga
     1021 ttcggccggg cagaggcggc ggcgagggtt gacgcccagg cggactccga ggagcgcagg
     1081 acggcggccg atgtcgtcgt ggacaaccac gggaacctgg aagatctccg ggagcagatc
     1141 gccgcactct gggacgtact gacttccgcc gccacccgag aatgacatac tcgcaatgct
     1201 cgggggcgtg acggtccgcg agccgagaga cttgtcgacg ccaggtttag ctgtctggcc
     1261 gggtcaagga gggtcacagt tggaatgccc acggtgtcgg gctgagcttc ccgcagtcgc
     1321 acacttctgc cacgtgtgtg gcctggatca gcgctcggcg gactttgctc gccggaagtc
     1381 ctttgctgcc cggcccgatg agccggtcgc ctcatttgcg ttgatctcat cgatcatgcc
     1441 gcgcggtgcg ggccagcgtc cgcagacgta tcggattgcc ttgaccatcg cgctcattgt
     1501 ggccttgatt gccgcgatct tcggcgcgat gcccatcgct gtgctggtgg cggcttttgc
     1561 cgttcccgtc gtctacatcg tctacatcta cgacgtgaac ctttgggagg acgagccact
     1621 catcgtgacc ggtctcgcct tcttattgac tggcgcactc acggtcggct tcaccatctt
     1681 gtggacctat ctccgcgggc cggttcccta cggcaccacc acgtatgagg ggtccctgag
     1741 cgcggcgcca acggtcggcg cttttctgct cgtcgcactt gcggtgccga tcgtcggcga
     1801 ggtaatccgc cagatcggtc cggtgctgtt ggcaagtcgt cctgagttcg acgacctgat
     1861 ggacggcctg acattcggcg taataagcgg cctcgcctat tcctgcttcg acacgctgac
     1921 ccggcactgg gatctgctga ccggaggcct gcagggcccg gagccgggtc tgtgggtatc
     1981 gctgatcttc ctcgagggct tcgtcaaacc gatgatcatc ggcacggcca gcggcatcgc
     2041 cgtcgcggag ttctccggtc tgggtcgtgg ctacgacggc ttcacaccgc gctatttccg
     2101 tggcgtcggg gaagccattc tggccaacgc cgcgtaccag gccggcacct accttttctc
     2161 ctttgtcggc aacgcaacgc tgggtgtcgt gctgagcatt ctctggg
//
LOCUS       DATAOZ010000632        24764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7615193, whole genome shotgun sequence.
ACCESSION   DATAOZ010000632 DATAOZ010000000
VERSION     DATAOZ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 24764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24764
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7615193"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VHR64_15720"
     CDS             complement(<1..980)
                     /locus_tag="VHR64_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304333.1"
                     /translation="MVAGKDGSSVCERLLSRRTLIQAGGGTALAAVALGDQFFARPHF
                     TTAQAISTIAVDLSQQGSEFAKGDMPAGGGFSTASADRGLTVQAGLRGAQYVSPVIKT
                     DFAMTHVGPNWSADYTGGRFDVEVRLSQDGKRWTEWTPVSIETFELDPDRPGKPGIYG
                     SLLNGYGATRMQYRIRFDTQNGPVSVRRMTMTAMNVNDGPRAKVLVPGDGGQALAGPY
                     IAKPRPITRAGWGCDESLRFDRSGNEIWGKEYATWRVVVPHHTATTNNYWDAAAEVRA
                     IYYYHAVTHGWGDIGYHALVGNNKQTYEGRKSPDGQVLAHDLMAGHVLQC"
     gene            complement(1267..2115)
                     /locus_tag="VHR64_15725"
     CDS             complement(1267..2115)
                     /locus_tag="VHR64_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX3304334.1"
                     /translation="MYPGQFEYQRPSSVQEAVAILAGDPEAKVLAGGHSLVPAMKLRL
                     AMPGTLVDIGRIAELKGIKVNGGATIGAMTTYDELQDSAELTNAFPVIKETVNIVGDP
                     AVRARGTLGGSLAHSDPAADFTAVMLALGASVKATGSNGEREISSDDLFVDLLTTSLE
                     PDEILTSISVPALNGAGAAYEKHAHPASGYAVVGVAAVVKVEGGNFTSARIAVTGATS
                     KATRSTAAEEALIGKPANADTIAAAAAKAADGLELNGDVYASAEYRGQLIKVLAKRAI
                     TRAAGL"
     gene            complement(2155..4536)
                     /locus_tag="VHR64_15730"
     CDS             complement(2155..4536)
                     /locus_tag="VHR64_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX3304335.1"
                     /translation="MVASVDAPERMFGKAIKRREDPRLITGAAAFLDDIRLPGMSHAA
                     LLRSPHGHARIRSIDTSAAAAAPGVIGVFTGEDFSDLNPLPCAWQAGGVQNNVNTPRV
                     LAIGEVHQVGDPIAVVVAEDAGAAADAVDLISVDYEVLPTVVDARAATEDGAPQLHEN
                     APNNIVMHWTCGKGADEVRAAIDGAEITLSQRIVNQRLIPNAMETRGSIGRYDPGTGE
                     YTLWATSQAPHVMRLLITAFVMGIPEQKLRVISPDMGGGFGSKIFLYFDMPLTLALAK
                     RVGRPVKYVEDRSENYMSTTHGRDHITDLEVGATRDGKILAMNVTTYANMGGYLSTIA
                     PGIPTTLYGRMLSGCYKVPNIHCDVYGVYTNTAMVDAYRGAGRPEAAFVIERTCDLVA
                     GAIGLDPAEVRRRNFIQPEDFPYDNGVGMLPYDSGNYEPALDRALEAVGYRELREEQK
                     RRIDNGASKLLGIGLSSYVEVCGVAPSKWIGLQGEGWGAGLWESANVKVHLTGKVVVT
                     TGSTPQGQGHETTFAQLVSDELGIPYDDIDVEHSDTLGAPFGYGTYGSRSLSVGGTAI
                     FKSVGKIKEKARRITAHLLEANFEDVVYENGKCFVKGSPESGKTIQEIALAASVGYDL
                     PREIEPFLDETTYYDPPNCTFPFGTHICVVEIDRDTGHAVPITYVAVDDVGNVLNPLI
                     VDGQVHGGIAQGLAQALYESAYYNEDGQLVTGTMSDYAVPKASMVPSYQMDKTCTPTP
                     VNPMGVKGAGEAGTIAATPAVANAVIDGLKHLGVKHIDMPMTPERVWRAMNPS"
     gene            complement(4603..5103)
                     /locus_tag="VHR64_15735"
     CDS             complement(4603..5103)
                     /locus_tag="VHR64_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX3304336.1"
                     /translation="MADTMVAPNMVTVTMTVNGKEYTQAVEPRTLLVHFIRENLGLTG
                     THIGCDTSSCGACTVHMNGSSVKSCTVLAVQADGAEITTIEGLGTADNLHPVQEGFWE
                     MHGLQCGYCTPGMIMAGVSLLKRNPNPSEAEIRHALEGNICRCTGYQNIVKAIQWAAD
                     KMPQNA"
     gene            5325..6605
                     /locus_tag="VHR64_15740"
     CDS             5325..6605
                     /locus_tag="VHR64_15740"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3304337.1"
                     /translation="MAVRVRPASKPTRRLRVGQIRRPAIGAGGTFSALQTNPLFRRLW
                     LASTGYSLSQWMQNIALGWLAYELTDSERFVGLVAFAAGFPFVIVSIPGGALLDRFDR
                     RSVLLISQALAAIIATGVAIDVLSGHATPWHLIVAGFLNGSLQAIITPSQQSIVPRLV
                     QPRDLQNALGLMSAGGNMTRVFGPALAGTLIAALGTGYPFLLQAIALSVAFAVIFTSD
                     FPRVPPSTSRLGLRVVSEGSRIIAARPDLRELFMLSALPALLVFPYLSFVNVYAEDIM
                     KIGSEGLGILLAASGIGAVIGGLMIAATRSNVGMGRRLYILTFVYCLLILSFAIHPVI
                     WVAIPSLLLAGLVGSYAFSGNNALIQQRITDEVRGRVMGTYLLTWGLMPLGALWMGEV
                     GEIWNIRIATGAGAVICAIATVILRVRSQELHTI"
     gene            complement(6602..7681)
                     /locus_tag="VHR64_15745"
     CDS             complement(6602..7681)
                     /locus_tag="VHR64_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304338.1"
                     /translation="MGLADRGGSSGYIGSAWGDYCRAWLTQQVGTSLAVGPDVHGTVE
                     SVVDIDAIPGLPAFASSRALQNPDFVIALGTSDGSILVAADAKFSIETAKPRQVSAEI
                     LTALLESPGTPVRAVVPEHGVERDGFFLTPDYELTHLVMNGTTGILRTAVSFDQVLLL
                     DVEPALLFPEAHLRSLIDALALVDEAGVNWESDLVAALYYGRCAFACIGCRIDETKPL
                     LGRTEVREIDDPGLLPELQKRGRVARSAWQLVQRWDQDAEEVRALRIKVHQAADIGIA
                     NRDLRSLVEREADRLGRPAPSMNRVRRELALWTNAELASQFGTVFYPVPDIARFLNDL
                     RRAVAELQPRVPEQVAEVVARSAPA"
     gene            7862..8131
                     /locus_tag="VHR64_15750"
     CDS             7862..8131
                     /locus_tag="VHR64_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304339.1"
                     /translation="MHDIPESPFDPYVSQLMSLIANNDQISSSTLAQVCVRELHWLPG
                     FCDVIIHMLRANGLIQVFEWEPGKLHISERGRRWVRASQETVTIR"
     gene            8145..9758
                     /locus_tag="VHR64_15755"
     CDS             8145..9758
                     /locus_tag="VHR64_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX3304340.1"
                     /translation="MTAQTRSGAELVVRALETAGVDTVFGIPGVHTLALYDALPKSGI
                     RHVLARHEQGIGFMADGYARATGRTGVAFVITGPGVTNIATALGQAYTDSSPVMVIAS
                     NNPRATVDGMRGSLHDLKDQLGVLRAVTKSAMRVNTARDAANAVLGSLAIAQTDRPLP
                     VAVEFPLDVLDEQTDVEPGRLIPAACRREPDQTAVDEAAKRLASAKRTVIYCGGGAVS
                     SAAQNAIVSIAEALNAPVITSIQGKGAIPADHPNYAGSAWGDGNAVDDLIRQADCMLI
                     FGSRMGAQATYDFTMTFPREVIRVDIDPVELTLNVNPTLAIQADSAAAATAIAAQLKG
                     HEHVEGGYSRQQIAEARASSEAGSYHAERREWPDAIRAALPRDGILVTDMTQMAYVAT
                     ATYPVYEPRTFLFPNGYGTLGFSLPAAIGAKIGQPDKAVVSVVGDGGFQYSMGELAVA
                     IQERIGLPIVIFNDNTYSAVKEAQKWEREARYNAVDLVGNPDFQKLAAAYGIPAVKVT
                     APGDLTNEIRTALGRDLPTIIEVPTEQWV"
     gene            complement(9727..11688)
                     /locus_tag="VHR64_15760"
     CDS             complement(9727..11688)
                     /locus_tag="VHR64_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304341.1"
                     /translation="MTRSRFKSDWILPVLALVALAVWAWQRLDFFETSRWVMTADGRT
                     VRMPNALATVDHPFHSARVGMFLDALGNGHMPHWVFSHQGGYPAEFYPFGSSLVDLAV
                     WCATLGQMSIPMIHTWAVAIVFVLPALGFLGIARLAGLPLWIAPVGLACHLCIRGWWW
                     SGGSYELIEWGLVTNVLAAALTFLAAIAVAAGLSRRNPRWLIVGAGLIAWAEFTNPRS
                     LIAIGTIAAAALIVWLVDRREAGLGFAWLIVPFALGLAGSAPLLFSLIRYDGLYYFVH
                     YSGYANLHEWLDSSIQAVSGPIFIAALVGLAITLVGQRSPVESMIGWTTVIYCALTAY
                     LVVIDWPSTWAEQLETTRLMPFQRLLMIALAAIAVGRISLWAAGRWADIPCAAIAALI
                     PILYVISPPSIIPESDHGLVRVPTMAAPGIVDLHAAVEQADDQAPPGTAILILGTTVS
                     WHDQMWATLWSDRLFFYDDWLWYWQREHVGDYDPETEHAYRVDSSTIDQSYLATHGIG
                     AIIVTGEARQSAAEASFLTPIRTGIYDAYLVNGGTTLATIDGSNLASEIDDETIRVSG
                     IQAGGTVTIRENWFPRWQASVDGKAVPVIHRSDGYMDITVPAGGARLTVTYAATALDW
                     IARAVALVSFVVGVAILVRATPTARSELR"
     gene            complement(11685..12035)
                     /locus_tag="VHR64_15765"
     CDS             complement(11685..12035)
                     /locus_tag="VHR64_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304342.1"
                     /translation="MIDEQPIEHRFDRRWIWCGLIAMVGLIAALIRVPTRLGVQVPET
                     RLPGFDPSLVDLSSHEHVWRVWWRARHEFISLHPQWLLTAGLAVLLVVFAAGVLCLVW
                     FALAPGPEPAETME"
     gene            complement(12032..13570)
                     /locus_tag="VHR64_15770"
     CDS             complement(12032..13570)
                     /locus_tag="VHR64_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304343.1"
                     /translation="MLEQATLAPTTGPSVHPDKRGRLAGFAAWPPHRKYIAVLLILFF
                     AKQVFNVVVFPPFTGHDEVAHFAYIRTVADEHRIPVIPDLAEWRAAWNARKSTYPGDY
                     LPLDLYKYQRYVLDWCCSAVDRPEFASQPPNAMSFAGETYPNGWQYAANHPPLYYAVM
                     APLYKATDSMSLERQQYVLRAAAIPIGLMVVWLTYLMAGLLFPGDRLMMMVAPTFVAF
                     QTQFSYESAMVNNDIMLIGVFTVLVYLMIRSVRQGFTLKSSALIGLVFGLGLLAKASM
                     LSAAPLIALAIVLGVGIRHVRRWVTLGAVSVVVAALVCWPWYAFLYRTYGNFSGLRQI
                     KALQYLWTYQFESPPSIWNLLFNKGFAEMRWRETWGEFGWRLIHLDTQLLMVIGIPLL
                     ILAIAAIGGLIAGSVQRLTGRTTGHSILVREQVIGLWVLLSVCVVAYGAMLQFGTDFR
                     LTQARYYFNAVSAVAILLAFGLRQIVPVRYHSAAIVVFLLFMISINVLIYTQYVIPYW
                     YLAS"
     gene            complement(13625..15115)
                     /locus_tag="VHR64_15775"
     CDS             complement(13625..15115)
                     /locus_tag="VHR64_15775"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX3304344.1"
                     /translation="MPAHSNGSGPEASDRSTLVMGAGPGGLCSAYVLSKAGVPTTVVE
                     KAPFVGGLARTIRHQTEHGEFKFDIGGHRWFTKNDELNDIFKEVVGEELLWVDRISRI
                     YFDGKFVDYPLKISNALKAVGPVMAAKAMADYGKVRVEQKLHPTPVVSMEDAYIDQFG
                     RTLYELFFQRYSEKVWGLPCSQMSGDWVAQRSKGMSLVTAVKDAIVPSKGKVVSLIDN
                     FMYPKYGFGRFSERMAGAITAMGNKIDLGSGVEVVHRDGNRVTGVTLSTPDGPKRVEA
                     ANYISSIPLTVLAKIVEPAAPPDVIEAANQLTFRNVITVNVMLKKRQVTPDTWLYVHD
                     RNILFGRFHEPKNWSAAMVPSDEYTSLVVEYFCSFGDSIWNMTEEQLVEQTVNHLVND
                     LKFITPDEVIGGFTIRAPRAYPSYVIGYEAHLEKVKNFVYSLENIQIIGRYGTFRYNN
                     TDHSIETGLLAAKNILGEHHDLDEVNADQEYHEIKRVEPALAGSRR"
     gene            complement(15324..15749)
                     /locus_tag="VHR64_15780"
     CDS             complement(15324..15749)
                     /locus_tag="VHR64_15780"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX3304345.1"
                     /translation="MISAEALPARAVQALSVAKRFQKFLIVGSFGLLVNQLMLVILHD
                     VAGIRLAAASPLAILASMAVTFVLNEAWTWHDRGSGPIVHRLGTYVPINSGGLLINWV
                     ILLYLAEHQGVHHLVANLIGAGLAAVWNFSLNNAITWRE"
     gene            complement(15842..17134)
                     /locus_tag="VHR64_15785"
     CDS             complement(15842..17134)
                     /locus_tag="VHR64_15785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX3304346.1"
                     /translation="MKRRQDRPDSASPGLLGGHLTRVVIAAMLTLSFCLGMGVDRFTA
                     DNADAGSSLQDAQGYDVLQQTWDLIQNDYVALDQVNEADLMYGAASGMVNALGDTGHS
                     RFLNPKEAERFNASSEGEFVGIGIQLDFTTGQPVVAFPLDGSPAEKAGIRAKDIIIEV
                     DGTSTEGLTQEQVQDLLLGKEGDPVSLKILRPSTGETLDFTIVRQKIEVNAVTYAMLP
                     DNVALIRLSQFSTGVTNEMKAAIRAVKRAGATSIILDLRDNGGGLVFEAIGVASQFIP
                     EGKTIYMYQEKGQDPRPVKTVPGGLATDMPMVTLINHGSASAAEITAGALSDNGRSPL
                     IGETTFGTGTVLTPYNLDDGSIVLLGTALWLEPDGEQIWKHGVAPDDEVSLPADADQI
                     RPSVGGEISSSELQASTDSQLKAAYQKITGKDFSADVR"
     gene            complement(17405..18355)
                     /locus_tag="VHR64_15790"
     CDS             complement(17405..18355)
                     /locus_tag="VHR64_15790"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX3304347.1"
                     /translation="MAVQHPTYLWLDGEQARWEDATVHVSELGWSTVGGVFEGIKAYW
                     SEADGELFVFRLREHLERLHRSMRLVRLPLPYSVQELTDAITRLLRDNEAREDTYIFP
                     MGYLKDSHNDRWNLLEQQSSLLIHTRPTPSHLFSGKALSAGVSSWRRISEDVMPPRVK
                     NLSNYRNGQLARMDALQSGYDTSIMLNQQGKVAEGPGACVVIVRNNKVITPDLTQSVL
                     ESITRDAIITLAREDLGLEVEERAVDRTELYVADEAFMCGTHAEITPVTSVDRFVVGD
                     GEIGPVTKQLEKALDNAFRGHDGRYAEWRTPVGVASTAAV"
     gene            complement(18482..20302)
                     /locus_tag="VHR64_15795"
     CDS             complement(18482..20302)
                     /locus_tag="VHR64_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX3304348.1"
                     /translation="MSDKPSLQDQLEIMKQRLETGGMNRRDVLKVAAAATGVAAVTGA
                     INYGDAAASPLTGRRRLRYAKPQAAEEQIFYHTGIYTDPTSFDWNQNLYCNAEEETFA
                     SVLQFNENLEAVADWAETFEPNADASVWTFHIRPDNKGWSDGTPVTAGDFVYSWRRQL
                     DPETKAAYAGFLFDIKYAEQFNTGATVDDANDPLNGKVPTGDDLGAKALDDWTLEVTM
                     AGPRAYFPQVVAYTAAVPAPKWKVEELGDKWALNENGEPIVSNGPFKVDEWNKGQSIK
                     MSKNEGYWDAENIKLTNVIDPIYPAANEVLLYESGSGDQRLDWAVLDASNFERFMADP
                     ELSKQVSPYVYPGIWMMLPQVTIAPFDKIEVRTALGHAIDRDRLATITNGLVTPAYCM
                     VPQGVFGFLDDPSLAEINNFDPQAAMDALKGTDFEGGKNWPEITMYMRADEEQYNANI
                     MAEDIVAQLKTNIGMDVKIQAVPQSNFSEQLYQNKWELVFIRWWSDYPDPNNSYGDMF
                     YSRKTSGKRQAYSSKTFDDLVLKGKAEPDPEKRLAIYLECEKTIQTDRGYIPLVYRVD
                     NYVFKPWVKGVQVNKFGQAVPDGNIYVSMLRHVSIEGRQG"
     gene            complement(20713..21696)
                     /locus_tag="VHR64_15800"
     CDS             complement(20713..21696)
                     /locus_tag="VHR64_15800"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX3304349.1"
                     /translation="MTTTNGGSGPILKVENLKKYFQVGGGLLSGKGLTIRAVDDVSFS
                     VTAGETFGLVGESGCGKTTLGQTVIRLYDPTEGSIIFNGTDISRLKPRAMRPVRRDIQ
                     MIFQDPSASLDPRMTIGSAIAEPLNINAKLSKNEKRDRVQELLRVVGLNSYFANRYPH
                     EFSGGQRQRIGIARALALNPKVVICDEPVSALDVSIQAQVLNLLKSLQEQFHLTYLFI
                     AHNLAVVAHISDRIGVMYLGKLVEVGRSREITESPRHPYTKALISAIPVPDPTRARNR
                     IILEGDVPSPARPPSGCRFHPRCPIAQPNCAVDEPELLEKSPGHWVACHYA"
     gene            complement(21712..22767)
                     /locus_tag="VHR64_15805"
     CDS             complement(21712..22767)
                     /locus_tag="VHR64_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3304350.1"
                     /translation="MVTEDEGNISNPVLDVRNLKTQFFTRAGVVYSVDDVSFQVGQGE
                     TLGIVGESGCGKSVTALSIMRLIPQPPGKIVGGEVLLNNGKETKDLVKLSNAAIRRVR
                     GNEIAMIFQDPMTSLNPVYTVGNQLMEPLQLHLGLDHKAARERAIDLLKRVGIPAAED
                     RIDSYPHQFSGGMRQRVMIAMALACNPKVLIADEPTTALDVTIQAQILDLMVGLNRDF
                     GTAIILITHDLGVVAEVCKRVNVMYAGKIVESGPSSKLFANPQHPYTMGLLKSVPSIG
                     DNVKEPLHPIGGLPPDLLAPPQGCRFRPRCPRAQPKCLEAPPLMETAPGQRARCWFPG
                     GEFTDVPGAESIQSAAD"
     gene            complement(22777..23697)
                     /locus_tag="VHR64_15810"
     CDS             complement(22777..23697)
                     /locus_tag="VHR64_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3304351.1"
                     /translation="MAVSVPPQSLADLGASDEVIVRNQSSLWGDAWRRLIRNKLAVLG
                     LIVVVTFILVAVFAPLIAPYGESEVVDPRLVRYHPTWTWPMGLDQNGRDIFSRIVWGS
                     RVSLFVGVVAYLIVLLIAIPVGSIAGYYGGWVDNVIMRAVDVVFTIPQVLLVLMFVNW
                     RGPGVTNIFLAIGIIGWVTEARLIRGQFLTLREQDYIRAARVAGANPLYIIGRHLLPN
                     SLTPIIVAATFGVPAAIFTEAAVSFAGVGIRPPQASWGQMVGIASQPGYIRTDPHMLL
                     FPVLAIGLTMLGFTFLGDGLRDALDPKGND"
     gene            complement(23716..24654)
                     /locus_tag="VHR64_15815"
     CDS             complement(23716..24654)
                     /locus_tag="VHR64_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3304352.1"
                     /translation="MLGFIIKRVLWLIPTLLVMTLLTYFIMELTPGSPFDLANANGIT
                     PEMIARLEHQYGLDKPWYERYVIYVKNALQGDFGESYSYRPQQVSDIIARTMPTSLWL
                     GTMATMFAVTFGMTLGVLAAVNQNGIIDYITVTISILFYSMPNFVMGFVLILLFAVWL
                     PSHGIDLGFRPSGWESPKDWVLPTIALGAAPLATIARYTRSSMIDVIRSDYVRTARAK
                     GLAQQKVVVKHVLKNALIPVVTLIGPIFAIVATGSFFVEKIFNVPGMGKFYVESMQTK
                     DQTMILAVVLIFGVFLAVMNILVDIVYALIDPRIRY"
BASE COUNT         4712 a         7789 c         7580 g         4683 t
ORIGIN      
        1 ttgcactgca gaacgtgacc ggccatcaag tcgtgcgcga gcacctgacc gtccgggctc
       61 ttgcggccct cataggtctg tttgttgttg ccgacgagtg cgtggtaccc aatgtcgccc
      121 cagccgtgcg tgaccgcgtg gtagtagtag atcgcccgga cttcagcggc cgcatcccag
      181 tagttgttgg tggttgcggt gtggtgcggc acgacgaccc gccaggtggc gtactccttg
      241 ccccagatct cgttgccgct tcggtcaaag cggaggctct cgtcgcatcc ccagccggca
      301 cgagtaatcg gacgcggttt tgcgatgtac gggccggcca gtgcctgacc gccgtcgccc
      361 ggcaccagca ccttggcacg cggtccgtcg ttgacattca tcgcggtcat cgtcatccgg
      421 cgcaccgaga ctggaccgtt ctgcgtatcg aagcgaatcc ggtattgcat gcgcgttgcg
      481 ccgtaaccgt tgaggagcga gccgtagatg ccgggcttgc cgggacgatc cgggtccaac
      541 tcgaaggtct cgatcgagac cggcgtccat tcggtccagc gcttgccatc ctgcgagagg
      601 cgcacctcga catcgaaccg tccgcccgtg taatcggcgc tccagttcgg cccgacatgt
      661 gtcatcgcga aatcggtctt gatcaccggg gaaacgtact gggcgccgcg caatccggcc
      721 tgcaccgtca gcccgcgatc agccgaggcg gtgctgaagc caccacccgc cggcatatcg
      781 cccttggcaa actcgctgcc ctgttgactg agatcgaccg cgatcgtcga gatcgcctgt
      841 gcggtcgtga agtgcggacg cgcgaagaac tggtctccga gcgcaactgc tgccagcgcg
      901 gtcccaccac cggcttgaat caacgtacgg cgactgagca gtcgctcgca aactgatgaa
      961 ccgtccttgc cagccaccat ggcggctccc ctcccgaccg attcaccgcg cacgcccagc
     1021 ccggcgcgct aaccgcggtg tgtcaaattc cgtttgcaat tggggacaga gatcgatttt
     1081 atgcccgcgt gatgatccct gcccgtcaaa acggacaatg tgcggtgagg acagaatatg
     1141 acaggcgctc gctgtttggc taccgtccga acaggaaatc cggatgggag gatcggtcgg
     1201 gagatggcac agtgagggct cgccattccg acgagcccca ccaattcgac tggttagctg
     1261 ctgaacctag agaccggccg cccgggtgat tgcgcgcttg gcgagcacct tgatgagctg
     1321 cccgcggtac tcggcggagg cgtaaacgtc accgttgagc tcgagtccat cggcggcctt
     1381 cgcggcagcc gcggcgatcg tgtcggcgtt agccggcttg ccgatcagcg cctcctcggc
     1441 cgcagtcgac cgggtcgcct tgctggtcgc gccggtcacc gcgatccgcg ccgatgtgaa
     1501 gttgccaccc tcgactttca cgaccgcggc cacgccgacc accgcatagc cggaagccgg
     1561 atgcgcgtgc ttctcgtagg cggcgccggc tccgttcagg gctggaacac tgatcgaggt
     1621 cagtatctcg tccggctcca atgacgttgt cagcaagtcg acgaacaggt catcgctgct
     1681 gatctcgcgc tcaccgttcg agccggttgc cttcaccgat gcgcccaagg cgagcatgac
     1741 cgccgtgaaa tcggcagccg gatccgagtg agccaacgag ccgccgagcg ttccgcgcgc
     1801 ccgtacggcc ggatcaccga cgatgttgac ggtctccttg atgaccggga atgcgttggt
     1861 caattcagcc gagtcctgca gctcgtcgta ggtggtcatg gcgccgatcg tcgcgccgcc
     1921 gttgaccttg atccccttga gctccgcgat acggccgatg tcgacgagcg tgccgggcat
     1981 cgccagccgc agcttcatgg ccgggaccag cgagtgacca cccgcaagaa cctttgcttc
     2041 aggatcgccg gcgaggatgg cgactgcctc ctgcaccgac gacggtcgct ggtattcgaa
     2101 ctgtcctgga tacacgttgt ctccttccgg gatgccccaa ctgatacgcg gattctacga
     2161 cgggttcatg gcacgccaga cccgttctgg cgtcatcggc atgtcaatgt gcttgacgcc
     2221 gaggtgcttg agaccatcga tgacagcatt tgcgaccgcc ggcgtcgcgg caatcgtccc
     2281 cgcctcgccg gcacccttga cgcccattgg attcacagga gtcggcgtgc aggtcttgtc
     2341 catctgatag ctgggaacca tgctggcctt cggaacggca tagtcgctca tcgtgccggt
     2401 cacaagctgg ccatcctcgt tgtagtaggc gctctcatag agcgcctggg ccagtccctg
     2461 cgcgatgccg ccatggacct gcccgtcaac gatcaacggg ttcaggacat tgccgacatc
     2521 gtcgaccgcg acgtacgtga tcggtacggc atgaccggtg tcgcgatcga tctcgacgac
     2581 gcagatgtgc gtgccgaacg gaaacgtgca gttcggcgga tcatagtagg tcgtctcatc
     2641 gaggaatggc tcgatctcgc gcggaaggtc gtaaccgacg ctggccgcca gcgcgatctc
     2701 ctgaatcgtc ttcccgctct ccggcgaccc cttcacgaag catttgccgt tctcgtagac
     2761 gacgtcctca aagttggcct cgagaagatg cgcggtgatc ctgcgcgcct tctccttaat
     2821 cttgccgacg ctcttgaaga tggccgtgcc gccgaccgat agcgatcggc tgccgtatgt
     2881 gccatatccg aagggcgcgc cgagtgtgtc ggagtgctcg acgtcgatgt cgtcgtacgg
     2941 gattccgagc tcgtccgaga caagctgcgc aaacgtcgtc tcatggccct gaccctgcgg
     3001 tgttgaccca gtagtcacga ccacctttcc ggtcaggtgc accttgacgt tggcgctctc
     3061 ccagagtccc gcgccccatc cttcgccctg gaggccaatc cacttcgagg gtgcaacgcc
     3121 gcagacctcg acatacgaag acagaccgat gccgagcaac ttgctcgcgc cgttgtcgat
     3181 ccggcgcttc tgctcctctc gcaactcgcg atacccgacc gcctcgagcg ctcgatcaag
     3241 tgccggctcg taattgccgc tgtcgtaggg gagcatccca acgccgttgt cgtacgggaa
     3301 atcctccggc tggatgaagt tgcgacgtcg gacctcggcg ggatcgagac cgatcgctcc
     3361 ggccaccaga tcacaggtcc gctcgatcac gaatgccgcc tcaggccgac cggcaccgcg
     3421 ataggcatcg accatggcgg tattcgtgta gacaccgtag acgtcgcagt ggatgttggg
     3481 caccttgtaa caacccgaca gcatccgacc gtagagcgtc gtcgggatgc ccggcgcaat
     3541 tgtcgaaaga tagccgccca tgttggcgta cgtcgtcacg ttcatggcca ggatcttgcc
     3601 gtctcgggtc gcgccgacct cgaggtcggt aatgtggtcg cgaccgtggg tcgtcgacat
     3661 gtagttctcg gaacggtctt cgacgtactt gactggccgg ccgacgcgct tggcgagcgc
     3721 cagggtcagc ggcatgtcga aatagaggaa gatcttgctg ccgaagccgc cacccatgtc
     3781 cggcgagatg acgcgcagct tctgctcggg aattcccatc acgaacgccg tgatcagcag
     3841 gcgcatgacg tgcggcgcct gcgatgttgc ccagagcgtg tactcgccgg tgccgggatc
     3901 gtaccgaccg atcgagccgc gcgtctccat cgcgttggga atcaggcgct gattgacgat
     3961 ccgctgcgaa agcgtgatct cagccccgtc gatcgctgcc cggacctcgt ccgcaccctt
     4021 cccacaggtc cagtgcatga cgatgttgtt cggcgcattc tcgtggagct gcggcgcgcc
     4081 gtcttcggtg gccgcgcggg catcgacgac tgtcggcagc acctcgtaat cgaccgagat
     4141 caggtcgaca gcatctgccg cggcccctgc gtcttccgcc acgacaaccg caatcggatc
     4201 gccgacctgg tggacctcgc cgattgcgag cacccgtggc gtgttgacgt tgttctgaac
     4261 tccgccggcc tgccaggcgc agggcagcgg attaaggtca gagaagtcct cgccggtaaa
     4321 gacgccgatc acgccgggcg ccgcagcagc ggccgaggtg tcgatcgaac ggatccgcgc
     4381 gtgaccatga ggagaacgaa gcaacgcggc atgcgacatt ccgggaagcc tgatgtcgtc
     4441 caggaatgcg gccgcgccgg tgatcaggcg cggatcttcc cgccgcttga tcgccttgcc
     4501 gaacatgcgc tcaggagcat cgactgacgc aaccatctcg ttatcccatc actggaacgg
     4561 tccgcgccgc accagcgcga cgcggattct caacgatcct ggctaggcgt tctgcggcat
     4621 cttgtcggcc gcccactgga tggccttgac gatgttctgg tatcccgtgc agcggcagat
     4681 gttgccctca agggcgtgcc ggatctcagc ctcggatggg ttcgggttgc gcttcaggag
     4741 cgagacaccg gccatgatca tgccaggtgt gcagtagccg cactggaggc cgtgcatctc
     4801 ccagaagcct tcctggaccg ggtgaagatt gtctgcggtg ccgaggccct cgatcgtggt
     4861 gatctccgcg ccgtcggcct ggaccgccag aacggtgcac gatttcacgc tcgagccgtt
     4921 catatggacg gtgcaggcgc cgcacgagct ggtgtcgcat ccgatatggg taccggtcag
     4981 cccgaggttc tcgcggatga agtgaaccag cagggtgcgg ggttcgaccg cctgcgtgta
     5041 ttccttcccg ttcaccgtca tcgtcaccgt gaccatgttc ggggcaacca tggtgtccgc
     5101 catttgctgc gcgctccctt cttcatccag cctcgagccg cgccttcgcg gcacgagaag
     5161 ttcgccgatg tctgccgatt gtggcgtgat tatatagccg tgcctgcgcg atttgtgcat
     5221 cgtctccctg aggccgtccg gtccgcggag cttcgttcgg caaaacttcg tgcgaatgtg
     5281 taggctatgc ggaacgcatt tgatctggat ggagctcgtc ccgcgtggcc gtcagggtcc
     5341 gacccgcatc aaaaccgacc aggcggcttc gagtcgggca gatcagacgc ccagccatcg
     5401 gcgcgggcgg aacattcagc gcgctccaaa ccaatccgct tttccgccgc ctctggctcg
     5461 cctcgaccgg ttactccctc agccaatgga tgcagaacat cgccctcggc tggcttgcgt
     5521 atgagctgac cgactccgag cgcttcgtcg gcctggtcgc gttcgcggca ggcttcccgt
     5581 tcgtgatcgt atcgattccc ggcggagcgc tgctcgacag gttcgaccgc agatcggtgc
     5641 tgctgatcag ccaggcgttg gcggcgatca tcgcgaccgg cgtggccatc gacgtactct
     5701 ccggccacgc caccccgtgg cacctgatcg tcgccggatt cctgaacggg tcgctccagg
     5761 ccatcatcac gccgtcccaa caatcgatcg tgccccggct ggttcagccg cgcgacttgc
     5821 agaacgcgct gggcctaatg agcgcgggcg gcaacatgac ccgcgtcttc ggtccggccc
     5881 tcgcgggcac tctgatcgcc gcgctcggca ctggttaccc attccttctc caggcaatcg
     5941 cgctctccgt ggcgttcgcc gtcatcttca cgtccgattt tccccgcgtg ccgccttcta
     6001 cgtcgcgcct gggactgcgc gtggtatcgg agggatcgcg gatcatcgcg gcgcgcccgg
     6061 acctgcggga gctcttcatg ctcagcgcgc tgccggcact ccttgtcttc ccctatctca
     6121 gcttcgtgaa tgtctatgcc gaagatatta tgaaaatcgg ctccgaggga ttgggcatcc
     6181 tcctcgcggc gtcgggtatc ggcgcggtga tcggcggcct catgatcgcg gccacccgga
     6241 gcaatgtggg catgggccgc cgcctctaca tcctcacctt cgtctactgc ctgctgatcc
     6301 tctcgttcgc gatccacccc gtaatctggg ttgcgatccc cagcctgctc ctggctgggc
     6361 ttgttggctc ctacgcgttc agcggcaaca acgccctgat acagcagcgg ataactgatg
     6421 aggtccgggg ccgcgtcatg ggcacctacc tgctgacctg gggcctgatg ccgttgggtg
     6481 cactctggat gggcgaggtc ggcgagatct ggaacatccg gatcgcgacc ggtgccggag
     6541 ccgttatctg cgcaatcgcg accgtgatcc tgcgcgtgcg gagccaggag ctccacacga
     6601 tctaggccgg cgcgctcctg gccacaacct cggcaacctg ctcaggaaca cgcggttgaa
     6661 gctcggcaac cgctcggcgc aggtcattca ggaaccgtgc tatgtccgga actggataga
     6721 agacggtccc aaactggctc gcaagctcgg catttgtcca gagcgccaac tcacgccgga
     6781 cccgattcat tgacggcgcc ggccggccca accgatcggc ctcgcgctca acaagactcc
     6841 gaaggtcccg gttcgcgatc ccgatatccg ctgcctggtg aaccttgatc cggagcgcgc
     6901 gcacctcctc ggcatcctgg tcccagcgct gcacgagctg ccaggccgat cgcgccaccc
     6961 ggccccgctt ctgcagttct ggcaacaagc cgggatcgtc gatctcgcgg acctcggttc
     7021 ggccgagaag tggcttggtt tcatcgatgc ggcagccgat gcaggcaaac gcgcaccgcc
     7081 cgtaatagag tgccgcaacc agatcgctct cccagttcac gccggcctca tcgaccagcg
     7141 caagcgcatc gatcaacgag cggaggtggg cttccggaaa gagaagcgcc ggctcgacgt
     7201 cgagcagcag aacctgatcg aagctgaccg ccgtgcgcag gatcccggtg gtcccgttca
     7261 tgaccagatg cgtcagctcg taatccgggg tcaggaagaa gccgtcgcgc tcgactccat
     7321 gttccggcac cacggcgcga accggcgtgc caggcgactc gagtagagcg gtcaggatct
     7381 ctgcgctcac ttggcgcggc tttgccgtct caatcgagaa tttggcgtcc gccgcgacca
     7441 gaattgaccc gtctgaggta ccgagcgcaa taacgaagtc cggattctgg agcgcgcggc
     7501 tggaggcaaa cgcgggtagc cctggaatgg cgtcaatatc gaccaccgac tcgaccgttc
     7561 cgtgaacgtc tggaccgacg gccaggctcg tgccgacctg ttgcgtcagc caggcacggc
     7621 aataatcgcc ccacgcgctg ccgatgtatc cgctcgaccc gccccggtcg gcaagcccca
     7681 tcagacgccg atcgagcaag gagcgaacaa gggacgattg cgggaactga tgccgcgcct
     7741 gcgcgcgcct gcttcgctgg ggtcctgacg agtctacgtt ccgtttgcca ctcacatatc
     7801 aagcatagcc gttcatcggc cgctcaggtg gtaccatccc gccatggcaa gcgacgcacc
     7861 agtgcacgac attccagaat ccccgttcga tccgtacgtc agtcagctga tgtcgctgat
     7921 tgcgaacaat gatcaaatct ccagctcgac gctggcgcag gtctgcgtcc gcgagctgca
     7981 ctggctgccc ggcttctgcg acgtgatcat tcacatgttg cgcgcgaacg ggttgatcca
     8041 ggtttttgaa tgggaaccgg gtaagttgca catctccgag cgcggccggc gctgggtgcg
     8101 ggccagccag gaaacagtca ccattaggta gaaggaatcg caggatgaca gcacagacgc
     8161 gatctggcgc cgagttggtc gtgcgcgcgc tcgagacggc cggagtcgac accgtcttcg
     8221 gtattccggg cgtgcatacg ctcgctcttt acgacgcact gcccaaaagc ggtatccgtc
     8281 acgtgctcgc gcggcacgaa cagggaatcg gattcatggc agatggctac gctcgcgcca
     8341 ccggcaggac cggcgtcgca ttcgtgatca cgggtcccgg cgtgaccaat atcgcgaccg
     8401 cgctcggcca ggcgtacacg gactcctcgc cggtaatggt catcgcgtcg aacaacccgc
     8461 gcgcgacagt ggatggcatg cgcggctcgc tccacgacct caaggaccag ctcggagtgc
     8521 tccgcgccgt taccaagtcc gcgatgcggg tcaacaccgc tcgtgacgcc gccaacgccg
     8581 tgctaggttc actggcgatc gcccagaccg accggccgct gccggtggcg gtcgagttcc
     8641 cgctcgatgt cctcgacgag cagacagatg ttgagccagg tcgactgatc ccggccgcat
     8701 gccggcgcga gccagatcag accgcggtcg acgaggcggc caagcggctg gcctctgcca
     8761 agcggacagt catctattgc ggaggcggcg cggtctcatc ggcggctcag aacgcgatcg
     8821 tgtcgattgc cgaagcgctc aatgcaccag tcattacctc gatccagggc aagggcgcaa
     8881 ttccggccga tcatccgaac tacgccggca gcgcctgggg cgacggcaat gcggtagacg
     8941 atctgatcag gcaggccgat tgcatgttga tcttcggctc ccgcatgggc gcccaagcga
     9001 cgtacgactt cacgatgacc tttccgcggg aggtcattcg cgtcgatatc gatccggtcg
     9061 agctgacgct gaacgtcaac ccgaccctcg ccatccaggc cgactccgcg gcagccgcca
     9121 ccgcgatcgc tgcccaactc aagggacacg aacacgtcga gggcgggtac agtcgccagc
     9181 agatcgccga agcgcgcgcc agctccgaag ccggctcata ccacgcagag cgtcgcgaat
     9241 ggccggacgc aatccgcgcg gctctgccgc gcgacggcat cctcgtcacc gacatgacgc
     9301 agatggccta cgtggccacc gcaacctacc cggtctacga gccgcgcacc ttcctctttc
     9361 cgaatggtta cgggaccctc ggattcagcc tgcccgccgc gatcggggcc aagatcggtc
     9421 aaccggacaa ggcggtcgtc tcggttgtcg gcgacggcgg cttccagtat tcgatgggtg
     9481 agttggcggt cgccattcag gagcgaatcg gcctgccgat agtgatcttc aacgacaaca
     9541 cgtactcggc cgtcaaggaa gcacaaaagt gggagcgcga ggccaggtac aacgccgtgg
     9601 acctcgtcgg taacccggac ttccagaagc tggcagcggc ctacggcatc ccggcggtca
     9661 aggttaccgc gcctggcgat ctgaccaacg agattcggac cgcgctcggc cgcgacctgc
     9721 cgacgatcat cgaagttccg accgagcagt gggtgtagcg cgaacaagaa tcgcgacgcc
     9781 gacaacgaac gacacgagcg cgacagcgcg ggcaatccag tccagcgcgg tcgccgcgta
     9841 agtgacggtc agcctggcgc ctccagccgg taccgttatg tccatatagc catctgatcg
     9901 atggatgacc ggcacagctt tgccgtcgac actggcctgc cagcgcggaa accagttctc
     9961 gcggatggtg accgttccgc ctgcctgaat gccggagacg cggatcgtct cgtcgtctat
    10021 ctcggatgca aggttgcttc catcgatcgt agccagtgtc gtgccgccgt tgaccaggta
    10081 ggcgtcgtaa atcccggttc ggattggggt cagaaacgac gcctctgccg ccgattgacg
    10141 cgcctcgccg gtcacgatga tcgcgccgat gccatgcgtg gccaggtagc tctggtcgat
    10201 cgttgagctg tcaacgcgat aggcgtgctc ggtttccggg tcgtagtcgc cgacgtgctc
    10261 acgctgccag taccagagcc aatcgtcgta gaagaagagc cgatcgctcc agagtgtcgc
    10321 ccacatctga tcgtgccagg agacggtcgt gccgaggatc aggattgcgg tccctggcgg
    10381 cgcctgatcg tccgcctgct caacggctgc gtgaagatct acgatgcccg gcgcggccat
    10441 cgtagggaca cgcaccaggc cgtgatcgga ttccgggatg atcgagggcg gcgagatcac
    10501 atacaggatc ggaataagcg ccgcaatcgc cgcgcatggg atatccgccc atcgaccggc
    10561 agcccacagg ctgattcgcc cgaccgcgat tgcggccagc gcgatcatca gcaaccgctg
    10621 gaacggcatc agccgggtcg tctcaagttg ctctgcccag gtagacggcc agtcgatcac
    10681 gacgagatat gcagtcagcg cgcaatagat gacggtcgtc cagccaatca ttgattcgac
    10741 gggcgagcgc tgcccgacca gcgtaatcgc caaaccaacc agcgcggcaa tgaagatcgg
    10801 cccggaaacc gcctggatcg aggaatccag ccactcgtgc aggttcgcgt atccgctgta
    10861 atggacgaag tagtaaaggc cgtcgtaccg aatcagcgag aacagcagcg gagcggaacc
    10921 agccaagccg agagcgaacg gtacgatcag ccacgcgaat ccaagcccag cctcgcgacg
    10981 atcgacgagc cagacgatca gtgccgcggc ggcgatcgtc ccgatagcga tcagggagcg
    11041 cgggttcgtg aactccgccc aggcgatcaa gcccgcgccg acgatgagcc atcgcggatt
    11101 ccggcggctg agtcccgcgg caaccgcgat ggccgccagg aacgtcagcg cggcggccag
    11161 cacatttgtg accagacccc attcgatcag ctcatacgat cctccggacc accaccaccc
    11221 ccggatgcac aggtgacagg caaggccaac cggcgcgatc cagagcggca gaccggccag
    11281 gcgcgcgatt cccaggaaac cgagcgccgg cagcacgaag acgatcgcga ctgcccaggt
    11341 gtgaatcatg gggatggaca tctgtccaag cgtggcacac cagacggcaa gatcgaccag
    11401 cgaactccca aacgggtaaa actcggccgg atagccaccc tggtggctga atacccagtg
    11461 cggcatatgg ccgttaccga gcgcgtcgag gaacatgccg acgcgtgctg agtgaaatgg
    11521 gtgatccaca gttgccagcg cgttcggcat gcgtacagtc cggccatcgg cagtcatgac
    11581 ccagcgggac gtctcgaaga agtcgagtcg ttgccacgcc cataccgcaa gcgcgaccag
    11641 cgccaggacc ggcagaatcc agtcgctctt gaatcgtgat cgcgtcattc catggtttcg
    11701 gctggctcag gtcccggcgc gagtgcgaac caaacgaggc aaagcactcc ggcagcaaag
    11761 acgacgagca acaccgcgag cccggcggtc agcaaccact ggggatgaag gctgatgaat
    11821 tcgtgtcgcg cacgccacca gacacgccag acatgctcgt ggctggaaag gtcgacgagc
    11881 gagggatcga agcctggaag tctggtctct ggtacctgga cgccgagccg cgtcggcacc
    11941 cggatcagcg ccgcaataag tccaaccatc gcgatcagac cgcaccagat ccaccgtcga
    12001 tcaaatcgat gctcgattgg ctgctcgtcg atcatgacgc caggtaccag tacggaatca
    12061 cgtactgggt gtagatcagt acgttgatcg agatcatgaa gagcaggaag acgacgatgg
    12121 cggccgagtg ataccgcacc ggcacaatct gccgtagtcc gaaggcgagc aagatcgcta
    12181 ccgcgctgac cgcgttgaag tagtaccgcg cctgggtcaa cctgaaatcg gtgccgaact
    12241 ggagcatcgc gccgtacgca acgacgcaga ctgagagcag cacccacagc ccaatcacct
    12301 gctcccgaac caggatcgaa tgtcccgtcg tccgccctgt gagtcgctgg acgctacctg
    12361 cgatcaatcc tccgatcgcg gcaattgcta ggatcaggag cgggataccg atcaccatca
    12421 ggagttgggt atcgaggtga atcaggcgcc agccaaactc tccccaagtc tccctccatc
    12481 gcatctcggc gaaacccttg ttgaacagca agttccagat cgatggcgga ctttcaaatt
    12541 ggtacgtcca gagatattga agcgccttaa tctgccgcaa tccgctgaaa ttgccgtagg
    12601 tgcggtatag gaacgcatac cagggccagc acacaagcgc cgcgaccaca accgacaccg
    12661 cacctaacgt gacccagcgc cggacatgcc tgattccgac gccaagcacg atggcaagtg
    12721 cgatcagcgg ggccgcgctc agcatcgacg ctttcgccag caggcccagt ccgaagacaa
    12781 ggccaatcag cgcgctcgat ttgagcgtga atccctgccg cacgcttcgg atcatcaggt
    12841 aaaccaggac cgtgaagacg ccgatcagca tgatgtcgtt gttgaccatc gccgactcat
    12901 acgagaactg cgtctggaat gcgacgaatg tcggcgcgac catcatcatc aggcgatctc
    12961 cggggaacag gagccccgcc atgagatacg tgagccagac gaccatcagc ccgatcggaa
    13021 tcgccgcggc gcgcaacacg tactgctgcc gctcgagcga catggagtcg gtcgccttgt
    13081 agagcggcgc catcaccgcg taatagagcg gcgggtgatt cgccgcgtat tgccagccat
    13141 tcgggtaggt ctcgccggcg aaggacatgg cattcggcgg ctgggaggcg aattcgggcc
    13201 ggtcaactgc gctgcagcac caatcgagca cgtacctctg gtacttgtag agatcgagcg
    13261 gcaggtagtc gccggggtag gtagatttac gcgcgttcca ggccgcgcgc cattccgcga
    13321 ggtctggaat gaccggaatc cggtgctcgt cggcaaccgt gcggatgtag gcgaaatggg
    13381 cgacttcgtc gtgtccggtg aacggcggga aaacgacgac gttgaagacc tgtttcgcga
    13441 agaacaggat tagcagaacc gcgatgtact tgcgatgtgg cggccaggct gcgaatcccg
    13501 ccaggcgacc gcgcttgtcc ggatgtacgg aagggcccgt tgtcggtgct aacgtcgctt
    13561 gctcaagcac ttcggcacgc tccggcaaca ggccatttcc gactcgacca gaaccaaccc
    13621 tggactagcg tcggctgcca gccagcgcag gttcgacgcg cttgatctcg tggtactcct
    13681 gatcggcatt gacctcgtcg agatcgtggt gctcgcccag gatgttcttg gcggccagca
    13741 gaccggtctc gatcgagtga tcggtgttgt tgtaccggaa ggttccatat cggccgatga
    13801 tctggatgtt ctccaggcta taaacgaagt tcttgacctt ctcgagatgc gcctcgtagc
    13861 cgatcacgta cgaggggtag gcccgcggcg cccgaatggt aaagccgccg atcacctcgt
    13921 ccggcgtgat gaacttcagg tcgttgacca ggtgatttac cgtctgctcg accagctgct
    13981 cctcggtcat gttccagatc gagtcgccga atgagcagaa gtactcgacc acaagcgagg
    14041 tgtactcgtc gctcggcacc atagccgcgc tccagttctt cggctcgtgg aagcgtccga
    14101 agaggatatt gcggtcgtgg acgtagagcc acgtgtccgg cgtgacctgc cgcttcttga
    14161 gcatcacgtt tacggtgatc acgttgcgga aggtgagctg gttcgccgcc tcgatcacgt
    14221 ccggcggcgc ggccggctcg acgatcttgg ccaggactgt cagcgggatc gacgagatgt
    14281 agttcgccgc ctcgacccgc ttcgggccgt ccggcgtgct cagggtgacg ccggtcaccc
    14341 gattgccgtc gcggtgcacg acctcgacgc ccgagccaag gtcaatcttg tttcccatcg
    14401 cggtgatcgc gccggccatc cgctccgaga agcggccgaa gccgtatttc gggtacatga
    14461 agttgtcgat cagtgagaca accttgccct tggacggcac gatcgcatcc ttgacggccg
    14521 tcacgagcga catccccttg gaccgctgcg caacccagtc accgctcatc tggctgcagg
    14581 gaaggcccca aaccttctcc gagtagcgct ggaagaagag ctcgtagagg gtccgtccga
    14641 actggtcgat ataggcatct tccatcgaga cgactggggt cgggtgcagc ttctgctcaa
    14701 cccggacctt gccgtagtcg gccatggctt tggcggccat gacaggccca actgccttga
    14761 gcgcgttgct gatcttgagc gggtagtcga cgaacttgcc gtcgaagtag atccggctga
    14821 tgcggtcgac ccagagcaac tcttcgccga cgacctcctt gaagatgtcg ttcagctcat
    14881 cgttcttggt gaaccagcgg tgcccgccga tgtcgaactt gaattcgccg tgctcggtct
    14941 gatgccggat cgtccgcgcc agaccgccca caaacggcgc cttctcgacg acggtggtcg
    15001 ggacgccggc cttgctcagc acgtaagcgg agcagaggcc accgggccca gctcccatta
    15061 cgagcgtcga gcggtcggac gcttccggtc cggaaccgtt ggaatgtgcg ggcatgcagc
    15121 taatgccttt ctcaacagaa gatatacaac acgggacaga tgcccgattc tcgacggttc
    15181 agagtgcgat ccctgtgctc gttatttcgc tgctcatgca gcgtacgtcg gcggatcgca
    15241 acaattctgt ataccactgg ccttttgtgc cgtgccaatg ccaccttgcg tgatcgccgc
    15301 aagctgaccg cctcgctcgg gacctactcc cgccaggtaa tcgcgttgtt caggctgaaa
    15361 ttccagactg ccgcgagacc agcgccgatc aggtttgcca ccagatgatg gacgccctgg
    15421 tgttcagcca ggtagagcag aatcacccag ttgatcaaca acccaccgga gttaatcgga
    15481 acgtaggttc ccaatcgatg tacgatgggt ccgctcccgc ggtcgtgcca cgtccaggcc
    15541 tcgttgagca cgaaggtcac ggccatggag gcgaggattg ccagcggcga ggcggcggcg
    15601 agccggatcc cggcgacgtc gtgcaggatc accagcatga gctgattgac gagcaagccg
    15661 aatgagccga cgatcaggaa cttctggaac cgcttggcga cggagagggc ctgtacggct
    15721 cgtgcgggaa gcgcctcggc tgaaatcatt gcgggtgacc ctgctccttg cgattgtgca
    15781 gaagatcgcc gcccgagcgg tacccgggcg gcgaagctat ttcgtaccct accacgacac
    15841 tctaccgaac gtccgccgaa aagtctttcc cggtgatctt ctggtaggcc gctttaagct
    15901 gggaatccgt cgacgcttgt agctcagacg acgaaatctc gccgccgacg gatggccgaa
    15961 tttggtcggc atcggcagga agcgacactt catcgtccgg agccacgccg tgcttccaga
    16021 tctgctcgcc gtccggctcg agccagagcg cggtgccgag caggacgatc gagccgtcat
    16081 cgaggttgta cggcgtcagg acggtgcctg ttccgaaggt cgtctcgccg atcagcggcg
    16141 agcggccatt gtcgctcagg gcgcctgccg tgatctcggc cgccgaggcc gagccgtgat
    16201 tgatcagggt caccatcggc atgtcagttg ccaggccacc cggaaccgtc ttgactgggc
    16261 gcggatcctg ccccttctcc tgatacatgt aaatggtctt gccctcgggg atgaactgcg
    16321 atgcaacgcc gatcgcctcg aagaccagac cgccgccatt gtcgcgcagg tcaaggatga
    16381 tcgaggtggc gccggcccgc ttgactgcac ggatcgccgc cttcatctca ttcgtgacgc
    16441 ccgtcgagaa ttgcgaaagg cggatcaggg caacattatc cggcaacatg gcataggtaa
    16501 cggcgttaac ctcaatcttc tgccggacaa tggtgaaatc aagtgtttcg ccggtcgatg
    16561 ggcgaagaat cttgagcgag acgggatccc cttccttgcc cagcagaaga tcctgaacct
    16621 gctcctgcgt caagccctcg gtcgaggtgc catcaacttc gatgatgatg tccttggccc
    16681 ggatgccggc cttctccgcc ggcgagccat cgagcgggaa cgcgacaacc ggctggccgg
    16741 tcgtgaaatc aagctggatg ccgatgccga cgaactcacc ttcggacgat gcattgaatc
    16801 gctccgcctc tttcggattg aggaatcgcg agtgtccggt atcaccgagc gcattcacca
    16861 tcccactggc cgcgccgtac atcagatcgg cctcgttgac ctgatcaagc gccacgtagt
    16921 cgttctggat aaggtcccag gtttgctgca ggacatcgta tccctgggcg tcctggagcg
    16981 atgagcctgc atcggcgttg tcagccgtga agcggtcgac tcccattccg aggcagaagc
    17041 tgagggtaag catggccgcg atcacgactc gcgtcaggtg gccgccgagt agcccggggc
    17101 tggccgagtc tggtcgatcc tgtcgccgtt tcattcacgt gctccttggt ccagaagacc
    17161 gactcgcctg cccatacaac gtgacggcga ttgactcggt tccggtctag attgtacgcg
    17221 ccgccggcta tggtccattc cggagagtaa ccgcgtaccg ggcatgtgag atatacgcga
    17281 atggcggccg atgagttcgc gcgaaccgga ccgcaacggt agcgcaggac cgttctgctt
    17341 cgcacggcgg ggaaacaaac acgcggggca gatccgcccc gcgtgtgacg aactcgaccg
    17401 aaccctagac ggcagcggtg ctggcaacgc cgaccggagt gcgccactcg gcgtaccggc
    17461 cgtcgtgtcc gcgaaatgca ttgtcgagcg ccttctcgag ctgcttcgtg accggcccga
    17521 tctcgccgtc gccgactaca aagcggtcga ccgatgtgac cggcgtgatc tcggcatgcg
    17581 tgccgcacat gaaggcctcg tcggccacat aaagctcggt ccgatcgact gcccgctctt
    17641 cgacctcaag tccgaggtcc tcgcgggcca gcgtgataat ggcgtcccgc gtgatgctct
    17701 cgagcacact ctgggtcaaa tccggggtaa tgaccttgtt gttgcgtacg ataacaacgc
    17761 atgcacccgg tccctcagcg accttgccct gttggttgag catgatcgag gtgtcgtagc
    17821 cagactggag cgcgtccatc cgggcgagct ggccgttgcg atagttcgac aggttcttga
    17881 cccgcggcgg catgacgtct tccgaaatcc gtcgccaact gctcacgccc gcgctgagtg
    17941 ccttgccgct aaacaggtgc gacggcgtcg gccgggtatg gatcagcagg ctcgactgct
    18001 gttcgagcaa gttccagcgg tcgttgtgcg agtccttgag gtagcccatg gggaagatgt
    18061 aggtgtcctc ccgagcctcg ttgtcgcgaa gcagccgcgt gatcgcatcg gtcagctcct
    18121 ggaccgaata ggggagtggc aggcggacca gccgcatcga gcggtgcagc cgctccaggt
    18181 gctcgcgcag gcggaagacg aacaactcgc cgtcggcctc gctccagtag gccttgatac
    18241 cttcgaagac gccaccgacg gtcgaccagc caagctcgga gacatgaacc gtcgcgtcct
    18301 cccaccgagc ctgctcgcca tcaagccaaa gatatgtcgg atgctgaacc gccacgccga
    18361 aatgctccca tgctgatatc gaggtttacg ttaccggcca ttatcgcact ggcgaccaaa
    18421 agaaatcggg agtggcgcca gtgccactcc cggtcgtcag tctcgattga gtgtgtcggg
    18481 actagccctg gcgaccctcg atcgagacat gccgcagcat gctgacgtag atgtttccgt
    18541 cgggcacggc ctggccgaac ttgttcacct gaacgccctt aacccacggc ttgaacacgt
    18601 agttgtccac gcggtagacc agcggtatgt agccgcgatc cgtttggatg gtcttctcgc
    18661 attccagata gatcgcgaga cgcttctccg gatctggctc cgccttaccc ttcagaacca
    18721 ggtcgtcgaa cgttttactc gagtaggcct ggcgcttgcc cgaggtcttg cggctgtaga
    18781 acatgtcgcc atagctgttg ttcgggtccg gatagtcact ccaccagcgg atgaagacga
    18841 gctcccactt gttctggtag agctgctccg agaagttcga ctgtggcacg gcctggattt
    18901 tgacatccat gccgatgttg gtcttgagct gcgcgacgat gtcctctgcc atgatgttcg
    18961 cgttgtactg ctcttcgtca gcgcgcatgt acatcgtaat ctcgggccag ttcttgccgc
    19021 cctcgaagtc cgtacccttg agcgcatcca tcgctgcctg tggatcaaag ttgttgatct
    19081 ctgcaagcga cggatcatca aggaaaccga acactccttg tggaaccatg caataagccg
    19141 gcgtgaccaa gccgttggta atcgtcgcga ggcgatcccg gtcgatcgcg tggccgagcg
    19201 ccgtgcgcac ttcgatcttg tcgaatggcg cgatcgtcac ctgcggcaac atcatccaga
    19261 ttcccggata gacgtacggc gatacctgct tggacaactc agggtccgcc atgaagcgtt
    19321 caaagttgga agcatccagg acagcccagt caagacgctg atcgcctgat ccggactcgt
    19381 acagtaggac ttcgttggcg gccgggtaga tggggtcgat gacattggtc agcttgatgt
    19441 tctcagcgtc ccagtatcct tcgttcttgg acattttgat cgactggccc ttgttccatt
    19501 cgtccacctt gaaaggaccg ttggatacaa tcggctcgcc gttctcgttg agcgcccact
    19561 tgtcgccgag ttcctcgacc ttccacttcg gcgccgggac ggcggcagtg tacgccacga
    19621 cctgcgggaa gtacgcacgt ggaccggcca tggtgacttc gagggtccag tcgtccagcg
    19681 ccttggcacc caggtcgtcg ccggtcggca ccttgccgtt caacggatcg ttggcgtcgt
    19741 cgacagtcgc accggtgttg aactgctcgg cgtatttaat gtcgaacaga aagcccgcat
    19801 atgcggcctt ggtctccgga tcaagctgtc ggcgccagga atagacgaag tcgccggcag
    19861 tgaccggggt cccgtcactc cagcctttgt tgtcgggacg gatgtggaag gtccaaaccg
    19921 atgcgtccgc gttcggctca aaggtctcgg cccagtcagc gacggcctcg aggttctcgt
    19981 taaactggag aacgctcgcg aaggtctctt cctcggcgtt gcagtacagg ttttggttcc
    20041 agtcgaagct tgtcgggtcg gtgtaaatgc ccgtgtggta gaaaatctgc tcttcagcgg
    20101 cttgaggctt cgcgtagcga agtcggcgac gaccagtcag cggagaagca gcagcatcgc
    20161 cgtaattgat ggcgccggtt accgcggcga ctccggtggc ggcggcggcc accttgagga
    20221 cgtcacggcg attcatgccg cccgtctcaa ggcgctgctt catgatttcc aactggtctt
    20281 gcaaagacgg cttgtctgac acaggtgctc ctccgtgtac aacaacgcac gcgtgccggg
    20341 tgctctcgat ctgcgcccgg atcgagccgg gcaacgtata ggttggctcg acgcgcgcaa
    20401 tgtagcatgt ttgcgtagaa acgagtaggg tcagttgccg cgggtgcatt cgtacctcgg
    20461 acgggacatt ggcaacgacg attagccttg caaacgcgct attggtagac gtacatcagc
    20521 gccggcgcca cacctctgac atcttccggg caaaatcaat cgcgtagcgc cagccccccg
    20581 tggccggcat ccggttgacg agatacacgg tcgatggagg cgatcgcgga gtcaaattgc
    20641 gcggtcgtca cccgggcagc ccggaggcgc gccgcgctac ggatttttgg gttggtctgc
    20701 ggcatacgga agctacgcgt agtgacaggc gacccaatgg ccgggcgact tctcgagcaa
    20761 ctccggctca tcgaccgcgc aattcggctg cgcgatcggg cagcgcgggt ggaatcgaca
    20821 accgctcggc ggccgggccg gactcggcac gtcaccctca aggatgatcc ggttccgcgc
    20881 ccgcgtcggg tccggcaccg ggattgctga gatcagcgcc ttcgtatatg gatgacgcgg
    20941 cgactccgtt atctcccgcg accttccgac ttcgaccagc ttgccgaggt acatgacgcc
    21001 gatccggtcg ctgatgtgcg cgacgacggc caggttgtgt gcaatgaaca ggtaggtgag
    21061 gtggaactgc tcctgcaggc tcttgagcag gtttagcact tgtgcctgga tcgaaacgtc
    21121 gagcgctgag actggctcgt cgcaaattac gactttggga ttgagcgcca gggcccgggc
    21181 aataccgatg cgctggcgct ggccaccaga gaactcatgt gggtagcggt tcgcgaagta
    21241 actgttcaag ccgacgaccc tcagcagctc ctgcactcgg tcgcgcttct cgttcttgct
    21301 cagcttggca ttgatgttga gcggctccgc tatggcgctg ccgatggtca tgcgcggatc
    21361 aagcgacgcc gaggggtcct gaaagatcat ctggatgtcg cgtcggacgg gccgcatcgc
    21421 ccgcggcttc agcctcgaga tgtccgttcc attgaatatg atggagcctt cggtcggatc
    21481 atagagccgg atcacggtct gaccgagtgt cgtcttgccg cagccggact cgccgaccag
    21541 accgaacgtc tcgcccgccg tgaccgaaaa gctgacatcg tcaaccgccc ggatggtaag
    21601 gcccttacct gacaagaggc cgccgccgac ctggaagtac ttcttcaggt tctccacctt
    21661 gagaatcggc ccggagccgc cgtttgtggt tgtcactgat tcactcatcg tctagtctgc
    21721 cgcgctctgg atggattcgg cgccgggtac gtcggtaaat tcgccgccgg ggaaccaaca
    21781 cctggctctc tggccgggcg cggtctccat cagcggcggt gcctcgaggc acttcggctg
    21841 cgcgcgtggg caccgaggcc gaaatcggca accctgcggc ggcgcaagca aatctggcgg
    21901 aagacctccg atcggatgca ggggctcctt gacgttgtca cctatcgacg ggacagattt
    21961 cagtaggccc atcgtgtagg gatgttgggg attggcaaac aacttcgatg atggcccaga
    22021 ctcgacgatc ttgccggcat acataacatt aacgcgcttg cagacttcgg ccaccacgcc
    22081 cagatcgtgc gtgatcaaga tgatggccgt tccgaagtcg cggttgagtc caaccattag
    22141 gtccaggatc tgggcctgga ttgtcacgtc gagcgcggtc gtcggctcat cggcgatcag
    22201 cacctttgga ttgcaggcga gcgccatggc gatcatcacg cgctggcgca tcccgccgga
    22261 gaactgatgc ggataagaat cgatccgatc ctcggcggcc ggaatgccaa cccgcttcag
    22321 aaggtcaatc gcgcgctccc tcgctgcctt gtggtcgagc ccaaggtgga gctgtaacgg
    22381 ttccatgagc tggttgccga ctgtatacac cggattcaat gaagtcattg gatcctggaa
    22441 gatcatcgcg atctcgttgc cccggacccg gcgaattgcc gcattggaga gcttgacgag
    22501 atctttggtc tccttgccat tgttgagcag gacctcgccg ccgacaatct tgcccggcgg
    22561 ctgtggaatc agtcgcatga ttgagagcgc cgtgacgctc ttgccgcagc cggactcgcc
    22621 cacgatcccc agtgtctcgc cctggccaac ctggaacgag acgtcgtcga ccgagtaaac
    22681 cacaccagca cgggtgaaga attgggtttt caggttccgg acatcgagta ccggatttga
    22741 gatgttgcct tcgtcctcgg tcaccattct tccgatctag tcattgccct tcgggtcgag
    22801 cgcatcgcga aggccgtcgc ccagaaacgt gaacccgagc atcgtcaagc caatcgcgag
    22861 gaccgggaaa aggagcatgt gcgggtctgt tcggatgtat cccggctggc tggcaatacc
    22921 aaccatctgg ccccaactcg cctgcggtgg gcgaatgccg acgccggcga agctgacggc
    22981 cgcttccgta aagattgccg ccggcacgcc gaacgtggcg gcgacgatga ttggcgtgag
    23041 cgaattgggt aacaagtgcc gcccgatgat gtaaagcgga tttgccccgg ccacgcgcgc
    23101 ggctctaatg taatcctgct cccgtaatgt caggaattgc ccgcgtatca gtctcgcttc
    23161 ggtgacccag ccgattatgc cgatcgcgag gaagatgttc gtaacacccg gaccgcgcca
    23221 gttgacgaac atcagcacca agagcacctg gggaatcgtg aacaccacgt ctacggcccg
    23281 catgatcacg ttgtcgaccc aaccgccgta gtagccggcg atggagccga ccgggatcgc
    23341 gatcagcagc acgatcaggt aggcaaccac acccacaaac agcgaaacgc gcgaacccca
    23401 aacgatacgg ctgaagatgt cgcgaccgtt ctggtccagg cccatgggcc aggtccaggt
    23461 cgggtggtag cgtacgagtc gcgggtcgac tacttcggac tcgccgtacg gcgcgatcag
    23521 tggagcgaag acggcaacga ggataaaggt aacgacgaca atcaacccga ggacggcaag
    23581 cttgttgcgg attagccgtc gccaggcatc gccccaaagg ctggattgat ttcgaacgat
    23641 gacctcatct gaggctccga gatcagcgag cgattgcgga ggaacactga ctgccattgt
    23701 ctcgttcagc cctgtctagt accggatccg gggatcgatg agcgcataca cgatatcaac
    23761 gaggatgttc atcacggcga ggaaaacgcc gaagatcaac accactgcca ggatcatcgt
    23821 ctgatccttg gtctgcatgc tttcaacata aaacttgccc atgcccggca cgttgaagat
    23881 cttctcgacg aagaacgagc cagtcgcgac gatcgcgaag atcgggccga tcagcgtgac
    23941 cacggggatt aacgcattct tgagcacgtg cttgacgacg accttctgct gtgccagacc
    24001 ttttgcccgc gccgtacgaa cgtaatcgga ccggatgacg tcgatcatgc tcgatcttgt
    24061 gtatcgcgcg atggtggcca atggcgctgc gccgagcgca atggtgggca ggacccagtc
    24121 cttgggactt tcccaccccg aaggtctgaa cccgaggtct atcccgtgac ttggtaacca
    24181 gaccgcaaat agcaggatca gcacgaaccc catcacgaag ttgggcatgc tgtagaaaag
    24241 gatcgagatc gtgaccgtga tgtaatcgat gatcccgttc tggttcacgg cggccagaac
    24301 gcctaaggtc atcccgaacg ttacggcgaa catcgtcgcc atggtgccga gccagagcga
    24361 cgtcggcatg gtccgagcaa tgatgtcgct cacctgttgg ggtcggtatg agtaggactc
    24421 gccgaaatca ccctgaagtg cgttcttgac atagatgaca tagcgctcgt accagggctt
    24481 gtcgagcccg tactggtgct ccagtcgcgc aatcatctca ggcgtgatgc cgttggcatt
    24541 cgcaaggtcg aagggactcc ccggcgtcag ctccatgatg aagtacgtta gcaacgtcat
    24601 cacaagcagg gtcgggatca accagaggac tcgtttgatg atgaaaccga gcattcgtct
    24661 taccccggtg ggcgggggcc tggaacctcg cccgattgct atgttcgctc ttgcttaggc
    24721 gggggcaata tagacgtgcg caatctgggg tgtcaacacg cgct
//
LOCUS       DATAOZ010000633         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4189701, whole genome shotgun sequence.
ACCESSION   DATAOZ010000633 DATAOZ010000000
VERSION     DATAOZ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4189701"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VHR64_15820"
     CDS             <1..399
                     /locus_tag="VHR64_15820"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX3304353.1"
                     /translation="TRKTVDAHSFKQYFAPRKPKSNWSKINKEKIERLTAEGRMAPAG
                     FAAIELAKANGSWSNLDEVEAMVVPDDLIVAIDATPGAREYFDSLSRMKLWEVLYWIN
                     GAKREVTRADRIRQIAEAAAQGKRPDRFRP"
     gene            526..1230
                     /locus_tag="VHR64_15825"
     CDS             526..1230
                     /locus_tag="VHR64_15825"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3304354.1"
                     /translation="MRSRLLMSFALLAAIFAAAFSATAPARALSCDPCPAVTTSALNL
                     RTAPSLNAEILLVIPNGAEVEYDNSVSRQNGYLAVSYNGVEGYAHSLYLLLFPTTAST
                     SDWLNLRSGPSITSSVILVMPPGSLVQVRSKAQNGYYAVAFDQRVRGYAHGDYRTFEH
                     TGAFSKGEGVFVQTDALNLRSSASASSSVRAVLLSGHRMIITGGPVERDGYSWYRVNA
                     GTSGSGWVAGEFLARR"
     gene            complement(1247..2542)
                     /locus_tag="VHR64_15830"
     CDS             complement(1247..2542)
                     /locus_tag="VHR64_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304355.1"
                     /translation="MYASGKSVTRRNNGVIRMLTLVALLACMWLAPLAPTRSTAAQES
                     PYAWIQQEMIEIRGLPLLHEVKERYITREEFRAELDNTEIADEVRTQTEGAERTMIAL
                     GLIPAGTDLFGATLEEQATGVVGYYDPDVKEMVMISDSGGTDPLAQITYAHEFVHALQ
                     DQHFDLNALQDQVEALDDSEAATAFRALVEGDATVAQFQFMIRHPELVSALDFSDTES
                     ASSPPVITEAIMFPYLAGSQFVTRLLREGIWEEVNIAYGHLPVSTEQILHFDRYVDGD
                     TPQDVSVPDIGSTLGRGWSIADQDIVGELVVMLMFAEEQPGQQSGFTAGFPDVATIAA
                     GGWDGDRYTTWVKGEQTVIVWQSAWDSRADAREFAGTMADYDAGRSGATAQGTASITA
                     IDGNEWSSRIVRSGDQVTYVLAPTAELADDVLAGVRDAA"
     gene            complement(2681..3064)
                     /locus_tag="VHR64_15835"
     CDS             complement(2681..3064)
                     /locus_tag="VHR64_15835"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX3304356.1"
                     /translation="MTRQAENKRNAMAFYDLMFNQSKPREAIERYVGQSYTQHNPGVG
                     DGTTAFIAYFERMAREYPGKRVQFRRAIAEDEYVVLHCHQTWPGDHDYAGIDIFRFDE
                     NGKIVEHWDVLQIIPETAANPNTMF"
     gene            complement(3048..>3289)
                     /locus_tag="VHR64_15840"
     CDS             complement(3048..>3289)
                     /locus_tag="VHR64_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX3304357.1"
                     /translation="WTFWLVIAAVVLALIASFVTQNYETVEVRLLFWSINVRLAWAVI
                     LAWLIGVLMGWLLPRRLRKPARLRGEGEVIHDPAG"
BASE COUNT          573 a         1073 c         1021 g          622 t
ORIGIN      
        1 acgcgcaaga ccgtcgacgc gcactcgttc aagcagtact tcgcgccgcg caagccgaaa
       61 agcaactggt cgaagatcaa caaggagaag atcgagcggt tgaccgccga ggggcggatg
      121 gcgccggctg ggttcgccgc gatcgagctg gcaaaagcca atggctcctg gtcgaatctc
      181 gatgaggtcg aggcgatggt cgtgccggac gacctgatcg tggcgatcga cgctactccc
      241 ggcgcccgtg aatacttcga ctcgctgagc cggatgaagc tctgggaggt cctctactgg
      301 atcaacggcg ccaagcgtga agtgacccgc gccgaccgca tccgccagat cgccgaggcc
      361 gccgcccagg gcaagcgccc ggaccgtttt cggccgtagc cacgctcgtc gtcaggttgg
      421 gtgcgaaacc ggttctttta tcgcttgtta cgatatgcag ggtggcaccc ttcaattcgc
      481 aatcagaggt ggctggactc tcgatgatcc ggggaggatg aatccatgcg cagccgactc
      541 ctgatgtcat tcgcgctgct cgcggccatt ttcgcggctg ctttctcggc gacggctcca
      601 gcgcgtgccc tgtcgtgcga tccctgtcct gccgttacca caagtgcgct caatcttcgc
      661 accgcgccga gtctcaacgc ggagattttg cttgtgattc cgaacggcgc tgaggtcgaa
      721 tatgacaaca gcgtctcccg gcaaaacggg taccttgccg tgtcctacaa cggcgtcgag
      781 ggatacgcgc acagcctgta cctgctgctg ttcccgacca cggcatcaac ttccgattgg
      841 ctcaacctgc gctccggtcc gagcatcacg tcgtctgtca tcctagtcat gcctccgggc
      901 agcctggtcc aggtccggtc caaggcgcag aacgggtact acgcggtcgc gttcgatcag
      961 cgcgtacgcg ggtacgcgca cggcgactat cgaacgttcg agcacaccgg cgccttttcg
     1021 aaaggcgaag gcgtgtttgt gcagaccgac gcactcaacc tgcgctcgag cgcgagtgcg
     1081 agcagttcag tccgggcagt gctcctcagc ggacaccgga tgatcatcac cggcggcccg
     1141 gtcgagcgcg atggctactc ctggtaccgc gtgaacgccg gcacgtccgg cagcggctgg
     1201 gtcgcgggcg aatttctggc tcgccgctga cgcgagatct gtagactcag gcagcgtccc
     1261 ggacgccggc cagaacatcg tcggcgagct cggcggtcgg ggccagcacg taggtcacct
     1321 ggtcaccact ccgcacgatc cggctcgacc actcgttgcc gtcgatcgcc gtgatcgagg
     1381 cggtgccctg cgcggtggct cccgatcggc cggcatcgta gtcggccatc gtcccggcga
     1441 attccctggc gtcggcccgg ctgtcccagg ctgactgcca gacgatgacc gtctgctcgc
     1501 ccttgaccca ggttgtgtat cgatcgccat cccagccgcc ggccgcgatc gtcgccacgt
     1561 ccgggaagcc ggctgtgaag cccgattgct ggcccggctg ctcctccgcg aacatcagca
     1621 tgacgacgag ctcgccgacg atatcctggt cggcgatgct ccatcctcgt ccaagcgtgc
     1681 tcccgatgtc cgggactgac acatcctgcg gggtatcgcc atcgacgtag cgatcgaagt
     1741 gcaggatctg ctcggtgctg accggcagat ggccgtaggc gatgttgacc tcttcccaga
     1801 tgccctcgcg cagcaacctg gtcacgaatt gggatccagc caggtacgga aacatgatcg
     1861 cctcggtgat caccggaggg gatgacgccg actctgtgtc gctgaaatcc agcgccgaaa
     1921 cgagctccgg gtgccggatc atgaactgga attgcgccac ggtggcgtcg ccctcgacga
     1981 gcgcccggaa cgcggtcgcg gcctcgctgt cgtccagtgc ttcgacttga tcctggagcg
     2041 cattgagatc gaagtgttgg tcctgcagcg cgtggacgaa ctcgtgggcg taggtgatct
     2101 gcgccagggg gtcggtcccg ccggagtcgc tgatcatcac catttccttg acgtccggat
     2161 cgtagtatcc gacgaccccg gtggcctgct cttcgagcgt cgcgccgaag aggtcggtcc
     2221 ccgcagggat cagaccgagc gcgatcatcg tccgctcggc gccctcggtt tgcgtccgga
     2281 cctcatcggc gatctcggtg ttatccagtt cagcccggaa ctcctcgcgg gtgatgtacc
     2341 gctccttgac ttcatgaagg agtggcaggc cccggatttc gatcatctcc tgctggatcc
     2401 aggcgtatgg gctctcctgc gcagcggtgg atcgggttgg cgcaagcgga gccagccaca
     2461 tgcacgcaag cagcgcgacc agcgtcagca tccggatcac cccgttgttc cgccgggtca
     2521 cgctctttcc actcgcatac attggctatc ctcaacgttt cgatgacagg cagggcggcg
     2581 cacgcccatc ccccgattat ccccgcatgg ggaagccctg cgcagcggac gttcggcaca
     2641 gtctgagcgt cgactatcgt cggattgccg cccgaggaga tcagaacatg gtgttggggt
     2701 tggcagcagt ctcggggatg atctggagga cgtcccaatg ctcgacgatc ttgccgttct
     2761 cgtcgaagcg gaagatatcg atcccggcgt agtcgtggtc gcccggccag gtctgatggc
     2821 agtgcaggac aacgtactcg tcttcggcga tcgcccgcct gaactggacg cgcttgccgg
     2881 ggtactcgcg cgccatccgt tcgaagtagg cgatgaaggc cgtcgtgcca tccccgactc
     2941 cggggttgtg ttgcgtatag gactggccga cgtaccgctc gatcgcctcg cgcggcttcg
     3001 actggttgaa catcaggtcg tagaaggcca tcgcattccg cttgttctca gcctgccggg
     3061 tcatggatca cctcaccttc accgcgcaac cgcgcaggct tgcgcaagcg acgcggcagc
     3121 aaccagccca tgagcacgcc aatcagccat gccagtatga ccgcccaggc caggcgcaca
     3181 ttgatgctcc agaacaggag tcgcacctcg accgtttcat agttctgggt gacgaagctc
     3241 gcgatcagcg ccagcactac cgccgcaatc accagccaaa atgtccatc
//
LOCUS       DATAOZ010000634         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7351248, whole genome shotgun sequence.
ACCESSION   DATAOZ010000634 DATAOZ010000000
VERSION     DATAOZ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7351248"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VHR64_15845"
     CDS             <1..271
                     /locus_tag="VHR64_15845"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX3304358.1"
                     /translation="DAALADVNAGRIGLVPRHLRDAHYPGAGRLGHGQGYAYAHDEPH
                     AVAAQQYLPDELAGARYYLPTEYGAEAPLVARLVRIEELLGRSDD"
     gene            complement(284..616)
                     /locus_tag="VHR64_15850"
     CDS             complement(284..616)
                     /locus_tag="VHR64_15850"
                     /inference="COORDINATES: protein motif:HMM:NF023257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3349 domain-containing protein"
                     /protein_id="HEX3304359.1"
                     /translation="MSQLSLPPFLSTVVDWLRAGYPQGVPEQDYIPLLALLARRLTSE
                     EVAAVAAELRREGVLPVTDTDIGELIMTITDELPRNEDVARVRAQLASGGWPLADPHS
                     ASEFPDGL"
     gene            complement(682..954)
                     /locus_tag="VHR64_15855"
     CDS             complement(682..954)
                     /locus_tag="VHR64_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304360.1"
                     /translation="MEILTKALDALWQVLLVGLLLGVGLPALFALGVRSLNTSRQLVS
                     VGSAGEEVTKPSPAGLAGGVVCFGLCILAVLFGIVVIVYGKQLFGV"
     gene            complement(963..2156)
                     /locus_tag="VHR64_15860"
     CDS             complement(963..2156)
                     /locus_tag="VHR64_15860"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX3304361.1"
                     /translation="MNEALFILCIVVVTALAFDFTNGFHDTANAMATSVATGALKPKA
                     AVALSAILNLVGAFLSIEVALTVSNKIIAIQGPNGAPVPGLMGVPMLVIILCGLVGGI
                     LWNLLTWLFGLPSSSSHALFGGLIGATIAALGLSGVKWEGVITSIVLPAVFAPVVAGL
                     VAGLGTWLVFKIVTGVPERRRDEGFRWGQVGSASLVSLAHGTNDAQKTMGVITLALVA
                     YGSWTNLHAIPLWVKIACAIAIAAGTWVGGWRIIRTLGKGLVEIKSPQGMAAETSSAV
                     IILSSSHLGMALSTTQVASGSILGSGVGRRVPVRWGVFGRMVVAWILTLPSAGIVGAL
                     CWFMANGIGGVFGVIVVFGILVLGAAYMFLRSRRSSITPDNVNAEWEGGLAPVPEREA
                     VKTAN"
BASE COUNT          516 a          809 c          746 g          394 t
ORIGIN      
        1 cgatgccgct ctcgccgatg tgaacgccgg caggatcggc ctcgtgcctc gccatctccg
       61 cgatgcccac tatcccggcg ctgggcgact cggacatggc caggggtacg cgtacgcaca
      121 cgacgagccg catgctgtcg ctgctcagca gtatcttccg gatgagctgg ctggcgctcg
      181 ctactacctt ccgaccgagt acggcgccga ggcgcctcta gtggccaggc tggtgcgcat
      241 cgaggagctc ttgggccgct ctgatgactg acggatagga gggctacagg ccgtccggaa
      301 actccgacgc ggagtgtgga tcagctaggg gccagccgcc tgatgccaac tgggcgcgga
      361 cccgcgcgac gtcctcgttg cgcggcaact catcggtaat agtcatgatg agttcgccga
      421 tatcagtgtc cgtgacaggc agcacacctt cgcgtcgcaa ttctgcggcg accgcagcga
      481 cttcttcgga cgtgagccgg cgggcgagca gcgcgagcaa ggggatgtag tcctgctcag
      541 gcaccccttg cgggtagccg gcccgcagcc agtcaacgac tgtgctgagg aacgggggca
      601 atgagagctg tgacaacgct agatcccttc tattgccgcc ggatcaaaga tcgcggcggt
      661 tcgtagcagc tggctgcaag gctagacgcc gaacagctgc tttccgtaga cgatgacgac
      721 gatgccaaac aggaccgcca gtatgcagag cccgaagcag acaacccctc ctgcgaggcc
      781 ggctggcgaa ggtttggtga cctcctcgcc agcggatccg acgctcacaa gctggcggct
      841 ggtattgaga gagcgaacgc cgagggcgaa cagggcaggt aatccgaccc ccaacagcag
      901 gccaactaga agcacctgcc acagcgcatc caaggccttg gtcaaaattt ccacaattct
      961 cctcagttcg cagtcttgac cgcctcgcgc tctgggaccg gcgccaatcc gccctcccac
     1021 tcggcattga cgttgtccgg ggtgatggac gagcgacggg accggaggaa catgtacgcg
     1081 gcaccaagca ccaggatgcc gaacaccacg atcacgccga agacaccgcc aatgccattg
     1141 gccatgaacc agcagagcgc tccgactatc ccggcactcg gcagagtgag gatccaggcc
     1201 acgaccatcc ggccgaagac gccccaccgg accgggaccc gacggccgac cccggatccc
     1261 aggatggagc cactggcgac ctgcgtagtg gacaaggcca tgccgaggtg actcgaagaa
     1321 agaatgatca ccgccgagga agtctcggca gccatcccct gcggcgactt gatctccacc
     1381 agccctttgc cgagggtccg gatgatccgc caaccaccta cccaggtgcc ggccgcgatg
     1441 gctatggcgc aggcgatctt tacccacagc gggatggcat gcaggttggt ccaactgccg
     1501 tacgcgacca acgcaagggt aatcacgccc atggttttct gagcatcgtt ggtgccgtga
     1561 gccagcgaca ccaaggaggc ggatccgacc tgcccccaac ggaatccctc gtctcgccgc
     1621 cgttccggca cgccggtcac gatcttgaac accaaccagg tgcccaggcc ggcgactaat
     1681 ccagcgacca ccggagcgaa caccgccggc aacacgattg acgtgatgac cccttcccac
     1741 ttgacaccgc tcaacccgag tgcggcaatc gtggcaccga tcaggccacc gaacagcgca
     1801 tgcgaggagg acgacggcag accaaatagc caggtcagca ggttccacaa gatgccaccc
     1861 accagcccgc acagaataat caccagcatc ggcacgccca tcaggcctgg cacaggcgcc
     1921 ccgttagggc cctggatggc gatgatcttg ttactcacag tcagagcgac ttcgatcgac
     1981 aagaaggcgc cgaccagatt caagatggcg gataacgcca ctgccgcctt gggcttcagg
     2041 gctcccgtgg caaccgaggt cgccatcgcg ttcgccgtgt catgaaagcc gttggtgaag
     2101 tcgaatgcga gcgccgtcac gacgacgatg cacaagatga acagggcttc gttcacaggc
     2161 acatcgtgct aggccacgga cctggcaatc cactccctac gtgccgccgg cagcatcgtt
     2221 catcgagaat tcacccctgc agtaactgtc gttcgcggac ggggttgcta cccaattacc
     2281 gggggtggtt cgaacccccg gaatcccagc ctcgccgcgg gaccgatcag ggtaaggtca
     2341 catagtcgcg ctgcgtggcc gctcagcgct gtccacaatg aagaaggaaa tcgatgtcgg
     2401 taggggagat cgccggtctg atcgctgcca ttgcgtttgt tgccttggtg gcattcgccg
     2461 ccgtg
//
LOCUS       DATAOZ010000635         5444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_508476, whole genome shotgun sequence.
ACCESSION   DATAOZ010000635 DATAOZ010000000
VERSION     DATAOZ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508476"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VHR64_15865"
     CDS             <1..862
                     /locus_tag="VHR64_15865"
                     /inference="COORDINATES: protein motif:HMM:NF025839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEX3304362.1"
                     /translation="YKGPWFAEQTDLGWLRTWGGGLLTTCGLDHALFFAEDTAEQYHY
                     EPKARENFGLHGRASNTPAKLTGYGHRWDGDTCILYAEAEVLQASMFGEHLILRRRIE
                     AVVGESKFTIHDEVENIGWDPTPHMLLYHVNIGFPVVDEGAEIVVPTRSINPRGDYPA
                     EGYRTLDAPEPAHFERVFEHDPIAESGGTVPVGIVNRERGIGAYELFNLNQLPFHFVW
                     RMLGEGSYVVGIEPSTNRTGGRLDARERGELIIMKPGDVRTYDLELGALVGTDEIDQF
                     TRRVTALGGV"
     gene            886..2286
                     /locus_tag="VHR64_15870"
     CDS             886..2286
                     /locus_tag="VHR64_15870"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX3304363.1"
                     /translation="MRRRDELSAIGAAEFRLNRRRLLRTGAAASGMAALGLIPSTIRP
                     GRFVRADTTINVLAPQWPQGPKEQALAGEWSKQSGIAVTFDAINYANLEQRVKLLVDG
                     ESADYDIYDYDSQWIGGFVQAGALERLDTPDYLGASGVTIRMGDFFPEITSRLCQYPT
                     SEPNAQPGPPAPVYGLPWSLNCEVLWYRTDLMDAPPATWEDLRNQAKAVTDKDVYGYA
                     TEGGRDADWIISEFAPIAWSNGGQLWDPANYTADGILNSAQNAASLKFLADMVNIDKS
                     IDPASANWTITERLGALLTGKAAMCLNWSPLFGGVADDETESQVAGKVGYAVSPKGSN
                     GQAALFGCQGTGINAWSQHKAEAWQYLQFWQSKETQAALMDDAPSGFVSARNDLREKV
                     THPWQKVFLDSIPYLRDIWNIPEYAQLLQILQTELNLAYVGQKEPQKALDDAAQAQQQ
                     VLDSSPGNPKNATPTA"
     gene            2362..3327
                     /locus_tag="VHR64_15875"
     CDS             2362..3327
                     /locus_tag="VHR64_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX3304364.1"
                     /translation="MEFQVGSTRRTRLGRELRAFPWLLIGPTVLLLTFLTVFPLLYSL
                     YLSLHSYQLGGSSDLVWFQNYDTLLTEGIFWRKLWFTLEITFFAVAIELVLGLLCAIV
                     LSKRLPGMGIFAMIVYIPMMISPLVVAYFWRIMLDGSFGVLTWFADSLGFGKPQWTIN
                     LTLAKVSLIATDVWQWTPFVTLLMLAGLQSVPEQLYEAASLDRASAWMVFTRITLPFL
                     RIPILLALLFRTIDTFKFFEAPYIITQGGPGDLTESLSLMAYNTGFEQSLLGLGAAIS
                     WLMLIVVYVIAWFLVKALTHQRRRAEEAIIDPNAPVIGASGSVLR"
     gene            3324..4160
                     /locus_tag="VHR64_15880"
     CDS             3324..4160
                     /locus_tag="VHR64_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX3304365.1"
                     /translation="MNRAPAGRSAFQRFIWYLPALAITAIFAFPLYWILLTSVKPRAL
                     IQDYPPRFRFSPVWDNYRLVFQSPYLDSIRNSVIIAVGSAILSVALATLTAYGLSRFS
                     FRSKDNLLFWILSLRMLPAIAVIIPYSRMVRWVNLEDTHAAMIMIYAVFNISFSVWLL
                     RGFFDEIPRELEEAAIMDGYGPWAVFGKVALPLVIPGVATALVFSMILSLNEFLLAFV
                     LTRTSAITAPVALTNVFITTIGVNYGGISAAAMVLVAPVALFAILVRRHLIRGMSFGQ
                     LG"
     gene            4162..5241
                     /locus_tag="VHR64_15885"
     CDS             4162..5241
                     /locus_tag="VHR64_15885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3304366.1"
                     /translation="MATLSLENLSLTYAQGIRAVQDLDLVVGAGEFCVVLGPSGCGKS
                     SLLRLIAGLQKPTTGLVRLDDQVINDVYPGERDIAMVFQHYTLYPNRTVREHLEFPLR
                     ARKLSASERYRRVADIAAMLHMSDLLDARPHQLSGGQAQRTAIGRALIRQPRLFLLDE
                     PLTSLDASLRFEMRTALKRIQSELGVTTIYVTHDQEEAFTMADRIVIMNEGRILQDAG
                     PEELYLRPKNLFVAGFVGAPAMNLLRGAVYGGKVIAGGFGLAIGDSPVVDGAVVVAGV
                     RPEDVQIGHGTSGGTGRIVVVEPQGDERIVSVVLDDVPEPVIWKVRAPKPNGSGEYQP
                     GQPVAMTVRHGGLHLFNGATGSRIV"
     gene            5255..>5444
                     /locus_tag="VHR64_15890"
     CDS             5255..>5444
                     /locus_tag="VHR64_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3304367.1"
                     /translation="MSTVEVRNLSIAFPERVVIRDLSFTVPSGELAVLIGPSGTGKTT
                     ILRAITGQVPLSSGQILID"
BASE COUNT          904 a         1850 c         1696 g          994 t
ORIGIN      
        1 ctacaagggc ccatggttcg ccgagcagac cgatctcggc tggctgcgga cctggggcgg
       61 tggcctgctg accacctgcg ggctcgatca cgccctcttc ttcgccgagg acactgccga
      121 gcagtaccac tacgagccaa aggcgcgcga gaacttcgga ctgcacggtc gcgcctcgaa
      181 cacgccggcg aagctgactg gctacggtca tcgctgggac ggcgacacct gcatcctcta
      241 cgcggaggcc gaggtgctgc aagcctcgat gtttggcgag cacctgatcc tccgccggcg
      301 gatcgaggcg gtcgtcggtg agtcgaagtt cacgatccac gacgaggtcg agaacatcgg
      361 ctgggatccc accccgcaca tgctcctcta ccacgtcaac attggcttcc cggtcgtcga
      421 tgagggtgcc gagatcgtgg tcccgactcg ctcgatcaac ccgcgcggcg attacccggc
      481 cgagggctac cggacgctcg acgcgcccga gccggcccat ttcgaacgcg ttttcgagca
      541 cgacccgatc gccgaatcgg gcggcacagt cccggtcggg atcgtcaacc gcgagcgcgg
      601 catcggcgcc tacgagcttt tcaacctgaa ccagcttccc ttccacttcg tctggcggat
      661 gctcggcgaa ggctcctacg tcgttggcat cgagccatcg accaaccgca cgggtggtcg
      721 cctcgatgcc cgcgagcggg gtgagctgat catcatgaag ccgggcgacg tccggaccta
      781 cgacctcgag ctcggcgcgt tggtcgggac cgatgagatc gaccagttca cgcgccgggt
      841 caccgccctc ggcggtgtct aggagcgcgg aaaggacggc tggcaatgcg gcgacgggat
      901 gagctgagcg cgatcggagc ggccgaattt cggctgaacc gtcgccgcct gctccgtact
      961 ggcgctgcgg cgagcggcat ggccgcgctc ggccttatcc cgtcgaccat ccggccgggt
     1021 cgcttcgtcc gcgccgacac gacgatcaac gtgctcgccc cgcaatggcc gcaaggcccg
     1081 aaggagcaag ccctggccgg ggagtggtcg aagcaatcgg gcatcgccgt caccttcgac
     1141 gcgatcaact acgccaacct cgagcagcgg gtcaagctgc ttgtcgatgg cgaaagcgcc
     1201 gactacgaca tctacgacta cgacagccaa tggatcggcg gcttcgtcca ggccggagcc
     1261 cttgagcgac tcgacacgcc cgattacctc ggggcgtccg gcgtcacgat caggatgggc
     1321 gactttttcc cggagatcac cagccgcctc tgtcaatacc cgaccagcga gccgaacgcc
     1381 cagcccggtc cgcccgcgcc ggtctacggg ctgccctggt cgctcaactg cgaggtcctc
     1441 tggtatcgca cagacctgat ggacgcgccg cccgcgacct gggaggacct ccggaaccag
     1501 gcgaaggccg tgaccgacaa ggacgtctac ggctacgcga ccgagggcgg ccgcgacgcc
     1561 gactggatca tctccgagtt cgcgcccatc gcgtggtcga acggcggtca actctgggac
     1621 ccggccaatt acacggccga tggcatcctc aacagcgcgc aaaacgcggc ctcgctcaag
     1681 ttccttgccg atatggtcaa catcgacaag tcgatcgacc cggccagcgc caactggaca
     1741 atcaccgagc ggctcggcgc actcctgacc ggcaaggccg cgatgtgcct gaactggtcg
     1801 ccgcttttcg gcggcgtcgc cgacgacgag accgaatcgc aggtcgccgg aaaggttggg
     1861 tacgccgtct cgcccaaggg aagcaacggt caggccgcgc tctttggctg ccaggggacg
     1921 ggcatcaacg cctggtcgca gcacaaggcc gaagcctggc aatacctcca gttctggcag
     1981 agcaaggaga cccaggccgc attgatggac gacgcgccgt cgggattcgt ctcggcccgg
     2041 aacgatctgc gggagaaggt gactcacccc tggcagaagg tcttcctgga ctcgatcccg
     2101 tacctcaggg acatctggaa catccccgag tacgcgcagc tactccagat cctgcagacc
     2161 gagttgaacc tggcctacgt cggtcagaaa gaaccgcaga aggcgctcga cgacgccgcg
     2221 caggcccagc agcaggtcct cgacagtagc ccgggtaacc cgaagaacgc gacaccgact
     2281 gcataaaagg atcgccagct caggcacgcg ggagtgactc gatacggtca ggatcggcga
     2341 atagtggctt cggcatcgtc cgtggaattt caggtaggca gcacgcggcg gacccgtctt
     2401 gggcgcgagc tgcgtgcctt cccctggctc ctgatcgggc ccaccgtcct gctcctgacc
     2461 ttcctgaccg tcttcccgtt gctctactcg ctctacctga gcctgcacag ctaccagctc
     2521 ggtgggagca gcgatctggt ctggttccag aactacgaca ccctgctgac cgaggggatc
     2581 ttctggcgca agctctggtt cacgctcgag atcaccttct tcgccgtcgc gatcgagctc
     2641 gtgctcggcc tgctctgcgc gatcgtcctc agcaagcggc tgcccggcat gggcatcttc
     2701 gcgatgatcg tctacatccc gatgatgatc tcgccactgg tcgtcgccta tttctggcgg
     2761 atcatgctcg acggctcgtt cggcgtcctg acctggttcg ccgattcgct cggcttcggg
     2821 aagccgcagt ggacgatcaa cctgaccctg gccaaggtct cgctgatcgc caccgacgtc
     2881 tggcaatgga ccccgttcgt gacgctgctg atgctggccg ggctgcaatc tgtcccggag
     2941 cagctctatg aggcagcctc gctcgaccgc gcgtcggcct ggatggtctt cacccgaatc
     3001 acgctgccct tcctgcgcat ccccatcctg ctcgcgctgc tcttccgcac gatcgatacg
     3061 ttcaagttct tcgaggcgcc ctacatcatc acccagggcg gacccggcga cctgaccgaa
     3121 tcgctctccc tgatggccta caacaccggc ttcgagcaat cgctgctcgg tctgggcgcg
     3181 gcaatctcgt ggctgatgct gatcgtcgtc tacgtgattg cctggttcct ggtcaaggcg
     3241 ctcacccatc agcggcgacg cgccgaggag gcgatcatcg atccgaacgc gccggtgatc
     3301 ggagcctccg ggagcgtttt gcgatgaatc gcgctccggc cggacgatcc gccttccagc
     3361 gcttcatctg gtatctgccg gcgctggcga tcacggcgat cttcgccttc ccgctctact
     3421 ggatcctgct gacctcggtg aagccgcgcg cgttgatcca ggactacccg cctcgcttcc
     3481 ggttctcgcc ggtctgggac aactaccggc tggtctttca gtcgccctat ctcgattcga
     3541 tccggaacag cgtcatcatc gcggtcggca gcgcgatcct ctcggtggcg ctggccacgc
     3601 tgaccgcgta tggtctctcg cgcttttcgt tccgatcgaa ggacaacctg ctcttctgga
     3661 ttctctcgct gcggatgctg ccggccatcg cggtgatcat cccgtactcc cgcatggtcc
     3721 gctgggtgaa cctggaggac acccacgccg ccatgatcat gatctatgcg gtcttcaaca
     3781 tctcgttctc ggtctggctc ctcaggggct tcttcgatga gatcccgcgc gagctggaag
     3841 aagcggcgat catggacggc tatggtccat gggcggtctt cggcaaggtc gcgctgccac
     3901 tcgtcatccc cggcgtcgcg accgcgctgg tcttcagcat gatcctgtcg ctgaacgagt
     3961 ttctgcttgc cttcgtcctg acccggacca gcgccattac cgcgccggtc gctctgacaa
     4021 atgtcttcat caccacgatc ggggtgaact acggtggcat cagcgccgcg gcgatggtgc
     4081 tggtcgcgcc ggtcgccctc tttgcgatcc ttgtccgccg ccatctgatt cgcggcatga
     4141 gcttcgggca gctgggctga tatggcaacg ctgagcctcg aaaacctgag cctgacctac
     4201 gcccagggga tccgcgccgt ccaggatctc gatctcgtcg tcggcgcggg ggagttctgc
     4261 gtcgtgctcg gaccgtccgg ctgcggcaag tcatcgctgc tccgcctgat tgccgggctt
     4321 caaaagccga ctacggggct cgtccggctc gatgatcagg tgatcaacga cgtctatccc
     4381 ggcgagcggg acatcgcgat ggtcttccag cactacacgc tttacccgaa ccggaccgtg
     4441 cgcgagcatc tcgagttccc actccgcgcc cgcaagctgt ccgcctcgga gcgatatcgc
     4501 agggtggctg acatcgccgc gatgctgcac atgtccgact tgctcgatgc gcgcccgcac
     4561 cagctctcgg gcgggcaggc ccagcgcacc gcgatcggtc gcgctctgat ccgccagccg
     4621 cgcctcttcc tgctcgacga gccgctgacc agcctcgacg cctccttacg gttcgagatg
     4681 cggacagccc tcaaacggat ccagtcagag ctcggcgtga ccacgatcta cgtgacccac
     4741 gatcaggagg aggccttcac catggccgac cggatcgtga tcatgaacga gggtcggatc
     4801 ctgcaggatg ccggcccgga ggagttgtac ctccggccga agaacctgtt cgtggccggg
     4861 ttcgtcggcg cccccgccat gaaccttctg cgcggcgcgg tctacggagg caaggtcatc
     4921 gccggtggat tcgggctggc gatcggggat tcgccggtgg tcgatggcgc ggtcgttgtg
     4981 gccggggttc gccctgagga tgtgcagatc ggccatggca cgtccggagg tacgggtcgg
     5041 atcgtcgtgg tggagccgca gggcgacgag cggatcgtct cggtggtgct cgacgacgtc
     5101 cccgagccag tgatctggaa agtgcgcgcc ccgaagccga acggcagtgg cgagtaccag
     5161 cccgggcagc cggtcgcgat gaccgtgcgt cacggtggcc tgcacctctt caacggcgcg
     5221 accggctccc ggatcgtatg aggatcggta cgtggtgagc acggtcgagg tccgcaatct
     5281 cagtatcgcc ttccctgagc gggtcgtgat ccgtgacctg agtttcaccg tgccctcagg
     5341 cgagctcgcg gtcctgatcg gcccttccgg caccggcaag acgacgatcc tgcgcgcgat
     5401 caccgggcag gtccccctct cgagtggcca aatcctgatc gacg
//
LOCUS       DATAOZ010000636         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9191923, whole genome shotgun sequence.
ACCESSION   DATAOZ010000636 DATAOZ010000000
VERSION     DATAOZ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9191923"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..>1669
                     /locus_tag="VHR64_15895"
     CDS             372..>1669
                     /locus_tag="VHR64_15895"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX3304368.1"
                     /translation="MQSAHRRFGDASRHPVLVLPALLGSLLLIAAGPLAESAGAGTTS
                     RPNILIFITDDQRAAGSMVEMPAVRRVFAQGGTQFPNGYVTTPMCCPSRSSIFSGQYA
                     HNTGIVVNDGSGFNSSDTWERYLHDNGYYTGLIGKYMNNVPTPGAPYFDFTDVGRNAT
                     DPEQVTIARAVSRFFQAESTHPAKPWALEVATYSPHEPWTSMPTTPTAVPPFVPPYRP
                     PSFQEADRTDKDISVQKRSYPDQLFEDQYKGQQLETQTANEEFANLWNTIQNRNQGSN
                     LLSFFLSDNGYAWGDHGLWGKGEPYVENSRVPFYVRWPGHFTAGAVDSRIAANIDIAP
                     TIYQATGINPGYTVDGRSLLDSWQRKWLLLEFQDPDIPQIPPWYSYLAPGDRQYIHWS
                     DGFIEDYNLSSDPAEMNASNAPDPSIEAKLAAAETCRGNS"
BASE COUNT          315 a          562 c          500 g          292 t
ORIGIN      
        1 ggatcggcga ccaggcggcc cgagcgacat ttgaccagcg gataactgcc ccggctctcg
       61 cgcgattggt gaggacggcc agcgggtaag ggctctcacc aactagtgcc cgacagggaa
      121 ggaggagcgc agtgtcgatc atcgcgattc tgctcatcat taccttggcc ttgctcgcta
      181 tctatctcgt gcggcgggtg atctaggcgg tcagagcgac cgctgcgatc tttgcgagct
      241 gaccctctga tgctcccgaa ctgggacgag ctagcccgcc tactggtgat ggcggtctgc
      301 ggctggggcc tgatgatcgg tgcgatctgg gcgatcgccc agatcgtcta gccgtgcatt
      361 caagcggtga gatgcagtca gcgcatcgaa ggttcgggga tgcctcgcgg cacccggtac
      421 tcgtcctccc tgcactcctc ggctctcttc tcctgatcgc cgccgggccg ctcgccgaga
      481 gcgctggcgc cggcacgacc agtcggccga acatcctcat cttcatcacc gacgaccagc
      541 gggccgccgg gtcgatggtc gaaatgcccg cggtgcgtcg ggtctttgcg cagggcggga
      601 cgcagtttcc gaacggctac gtgacgacgc ccatgtgttg cccgtcccgc tcctcgatct
      661 tctccgggca gtacgcacat aacaccggca tcgtcgtcaa cgatgggagc ggcttcaatt
      721 cgagcgacac ctgggagcga tacctccacg acaacggcta ttacacgggg ctgatcggca
      781 agtacatgaa caacgtgccg acgccgggtg ccccctactt cgatttcacc gatgtcggtc
      841 gcaacgccac cgaccccgaa caggtgacca tcgcccgggc ggtctcgcgc ttctttcagg
      901 ccgagagcac gcacccggca aagccgtggg cgctcgaggt ggcgacctac tcgccgcatg
      961 agccatggac gtcgatgcca accactccaa ccgccgtgcc cccattcgtc cctccgtacc
     1021 ggccgccctc cttccaggag gcggatcgca ccgacaaaga catctccgtt cagaagcgca
     1081 gctatcccga ccagctgttc gaggaccaat acaagggtca gcagctcgag acgcaaaccg
     1141 ccaacgagga gttcgcgaac ctctggaaca cgattcagaa ccgcaatcag gggagcaatc
     1201 ttttgtcgtt cttcctgtcc gacaacggtt acgcgtgggg agaccatggc ctgtggggca
     1261 agggggagcc ctacgtcgag aacagtcggg tcccgttcta cgtccgctgg cccggccact
     1321 tcaccgccgg tgcggtggac tcgcggatcg ccgccaacat cgatattgcg ccaaccatct
     1381 accaagccac ggggatcaat cccggctaca cggtcgacgg gcgctcgctg ctcgacagtt
     1441 ggcagcgaaa gtggcttttg ctcgagttcc aggaccccga tatcccgcag atcccgccct
     1501 ggtactcata tctggcgccg ggcgaccgcc agtacatcca ctggtccgac ggcttcatcg
     1561 aggactacaa cctaagcagc gatccggcgg agatgaacgc cagcaacgcc ccggacccct
     1621 ccatcgaggc gaagctggct gcggcagaaa cctgccgcgg aaacagctg
//
LOCUS       DATAOZ010000637         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6035122, whole genome shotgun sequence.
ACCESSION   DATAOZ010000637 DATAOZ010000000
VERSION     DATAOZ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6035122"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VHR64_15900"
     CDS             <1..332
                     /locus_tag="VHR64_15900"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEX3304369.1"
                     /translation="QTGWQLGFLAGPLFADRRYHEYFYAVAPQYATAQRPAYEPKGGY
                     AGTQFLTSLTKRFPGYWVGSYVRYDTLSGAAFAASPLVKENSYWSAGVAVAWMIKQST
                     RLVEANE"
     gene            350..1144
                     /locus_tag="VHR64_15905"
     CDS             350..1144
                     /locus_tag="VHR64_15905"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX3304370.1"
                     /translation="MLRLFKTATDYVLLYFSLVLLGLICLSWSVIALALYPLLPRRQG
                     IAAGRFGIMFGFRIYSWYLQLTGCYRLDLKAIDTLRGGPPVILAPNHPALIDALLILT
                     RHPNIACVMKGELLRNVFLGAGSRLARYIPSNNPRQMIKEAVADLREGGTVLLFPEGT
                     RTTRYPINPLTASVGVIAKRANVPVQTLIIETDSPYLSKGWPLFRRPQLPITYRVRLG
                     RRFDPPGDVAAFMVTLEQEYTQQLTSSVVPERLNEANAPHAGGVAR"
     gene            1165..>1611
                     /locus_tag="VHR64_15910"
     CDS             1165..>1611
                     /locus_tag="VHR64_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX3304371.1"
                     /translation="MAAAPASPTHLVLIPSYNPGPKVFDTVRAARERWNPVWVVVDGS
                     TDGTGAQLEALARQDAGLRVIIRPHNGGKGAAVLDGMVAARSAGFTHVLTMDSDGQHP
                     ADCIPAFMAASNASPAALVLGDPVFDASAPSVRVKGRKISNWWANLE"
BASE COUNT          277 a          519 c          516 g          299 t
ORIGIN      
        1 gacaaacggg ttggcagctt ggcttcctgg cgggaccgtt gtttgcggac cggcgctatc
       61 acgagtattt ctatgcggtc gcgccccaat acgcgaccgc gcaacggcca gcgtacgaac
      121 ccaagggagg ttatgctggc acgcagtttc ttacgtcgct gacgaagcgc ttccctggct
      181 attgggtggg ctcttatgtc cgctacgata cgctttcggg cgcggcgttt gccgcaagcc
      241 cgctcgtgaa ggaaaacagc tactggtcag caggggtggc ggttgcatgg atgatcaagc
      301 agtccacgcg cctggtcgag gccaacgagt aagcggcaag ccatacacca tgctgcgcct
      361 gttcaagacc gccaccgact acgtcctgct gtacttcagc ctcgtgctgc tcggcctgat
      421 ctgcctgagc tggtccgtga tcgccctggc gctgtatccg ctgctgccgc gccggcaggg
      481 catcgccgcg ggccgcttcg gaatcatgtt cggcttccgc atctactcgt ggtatctgca
      541 gctgacgggc tgttatcggc tcgacctgaa ggcgatcgac accctgcgcg gcggtccgcc
      601 cgtcatcctc gcgcccaacc atccggcgtt gatcgatgcg ctgctgattc tcacacgcca
      661 tcccaacatc gcgtgcgtga tgaagggcga gctgctgagg aatgtcttcc tgggagccgg
      721 ctcgcgcctc gcccgctaca tccccagcaa taatccgcga cagatgatca aggaggcggt
      781 cgcggatttg cgcgaaggcg gcacagtgct gctgtttccc gagggcacac gcacgacgcg
      841 gtacccgatc aatcccctga ccgcgagcgt cggggtcatc gcaaagcgtg cgaacgtccc
      901 ggtccagacg ctcatcatcg agacggattc gccctacctc agcaagggct ggccgttgtt
      961 ccgccgtccg caattgccta tcacctatcg cgtgcgtctg gggcgccgct tcgatcctcc
     1021 tggcgatgtg gccgccttca tggtgactct cgaacaggag tacacgcagc agttgacgag
     1081 cagtgtggtc cccgagcgcc tcaacgaagc gaacgccccg catgccggcg gcgtcgctcg
     1141 atgagtgaac gatcgcagtt accggtggct gcggccccgg cctcacccac gcatctggtc
     1201 ctgattccca gttacaaccc cggtccgaag gtgttcgata cggtcagggc ggcgcgggag
     1261 cgctggaatc ccgtgtgggt ggtcgtggac ggcagcacgg acgggaccgg agcacaactg
     1321 gaagccctgg cgagacagga tgcggggctg cgagtgatca tccggcctca caacggcggc
     1381 aaaggcgcgg cggtactgga tggaatggtg gccgcccggt cagccggatt cacgcatgtc
     1441 ctgacgatgg actcggacgg ccaacatcca gccgactgca tcccggcttt catggccgcc
     1501 tcgaacgcct caccggccgc gttggtgctg ggcgacccgg tgttcgacgc gagcgctccc
     1561 agcgtgcggg tgaagggacg caagatctcc aactggtggg cgaatctcga g
//
LOCUS       DATAOZ010000638         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1560351, whole genome shotgun sequence.
ACCESSION   DATAOZ010000638 DATAOZ010000000
VERSION     DATAOZ010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560351"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VHR64_15915"
     CDS             complement(<1..204)
                     /locus_tag="VHR64_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304372.1"
                     /translation="MLPTERLDYSAISERRPLALPDGARLAVWVIVNVEEWDINETMP
                     RTVLTPPAGGAPMPDIPNWAWHEY"
     gene            complement(350..760)
                     /locus_tag="VHR64_15920"
     CDS             complement(350..760)
                     /locus_tag="VHR64_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304373.1"
                     /translation="MDRDATVLAYLQVERGGADMCDCSGCRNFRLARAQAFPRDFLAL
                     LDELGIDALKDAEVSHVARLASGHHIYEGWYHFVGALEHTGDFPPVHFGDAFRVWMCH
                     ASAPRLPSLKGKPVVQLEFDARAVPWLLDEEELP"
     gene            complement(805..>1606)
                     /locus_tag="VHR64_15925"
     CDS             complement(805..>1606)
                     /locus_tag="VHR64_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX3304374.1"
                     /translation="LVVWTIVNLEFWDIARPMARQVLPAPTGQVLLPDVPNWSWHEYG
                     MRVGVWRFFELFRRLQIRPTLSINARVCEDYERVARAAHEAQWEFMGHAYEQGPVHLI
                     EDQKAMIDRSVAVIERFTGSKPVGWLGPGLTQTYDTPELLVGAGIKYIGDWVYDDEPT
                     EIATANGPLVTLPYTVELNDIPMMLVQHHESEYFTGRCIDTFDRLYHEGKDRAKIMAI
                     AIHPYISGQPHRIKYLEQVYEHLNRHSGVLHCNGVEILDWYLSTKRLA"
BASE COUNT          301 a          492 c          506 g          307 t
ORIGIN      
        1 gtattcgtgc caggcccagt tcgggatgtc cggcatcggc gcgccgccgg cgggcggggt
       61 caagacggtg cgcggcatcg tctcgttgat gtcccattcc tcgacattga cgatgaccca
      121 gaccgcgagc cgcgcgccgt caggaagcgc cagcgggcgg cgctcgctga tcgcggaata
      181 atccagccgt tcggtcggga gcattcaggg attcctctta agatcgtgat cccggcgaag
      241 gccgggatcc atttgtcaga ggcaagagcg actgaaccgt gggtcccggc cttcgccggg
      301 actttcggtt ttgagtgcgt gcgtcgcagg ctgtcgggaa agctggacct cagggtagtt
      361 cttcctcgtc taggagccag ggcactgctc tggcgtcgaa ttccagctgc acaacgggtt
      421 ttcccttcag gcttggcaac ctaggtgccg atgcgtgaca catccaaact ctgaaggcat
      481 cgccgaaatg aaccggcgga aaatccccgg tgtgctccag agcgcccacg aagtgatacc
      541 agccctcata aatatgatgg ccggaagcaa gccgggcgac gtgagacact tccgcatctt
      601 tgagagcgtc gatccctaat tcgtccagca gtgcgagaaa atcgcgtgga aatgcctgcg
      661 cccgcgcgag cctgaaattt ctgcagcccg agcagtcgca catgtcggca cctccgcgct
      721 cgacttgcag gtaagcaaga accgtcgcgt cccgatccac gctgtacttc catcggccaa
      781 agctaggcat aaacctggac ccgtttaggc gagccgcttt gtcgagagat accagtccag
      841 gatctcgacg ccgttgcagt gcaacacgcc ggaatggcgg ttgaggtgct cgtagacctg
      901 ttcgaggtat ttgatgcggt gcggctggcc cgagatgtag ggatggatcg cgatcgccat
      961 gatcttggcg cgatctttcc cctcgtgata cagccggtcg aaggtgtcga tgcagcgccc
     1021 cgtgaaatat tcggattcgt ggtgctgcac gagcatcatc gggatgtcgt tcaattcgac
     1081 cgtgtagggc agggttacga gcggcccgtt cgcggttgcg atctcggtcg gctcgtcgtc
     1141 atagacccaa tcgccgatgt atttgatgcc ggcgccgacg aggagctcgg gcgtgtcgta
     1201 ggtctgcgtc aggccggggc cgagccagcc aaccggcttc gacccggtga agcgctcgat
     1261 gacagcgacc gaacggtcga tcatcgcctt ctgatcctcg atcagatgca ccgggccctg
     1321 ctcataggcg tggcccataa actcccattg ggcctcgtgt gcggcgcgcg cgacccgctc
     1381 gtaatcctcg cagacgcgcg cgttgatcga gagcgtcggc cggatctgca gccggcggaa
     1441 cagctcgaag aagcgccaca ccccgacccg catgccgtat tcgtgccacg accagttcgg
     1501 cacgtccggc aacaagacct ggccggtcgg cgccggcagc acttgccgcg ccatcggccg
     1561 cgcgatgtcc cagaattcga ggttgacgat cgtccagacg acgagt
//
LOCUS       DATAOZ010000639         5251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7352621, whole genome shotgun sequence.
ACCESSION   DATAOZ010000639 DATAOZ010000000
VERSION     DATAOZ010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7352621"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..870)
                     /locus_tag="VHR64_15930"
     CDS             complement(160..870)
                     /locus_tag="VHR64_15930"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX3304375.1"
                     /translation="MDLTGFQALSFDCYGTLIDWESGIWEALQPWVERTGLEIDREEL
                     LAQFGGVETIIETRHPDLRYPLVLAETLRTMATNLNVDATDEECAAFGASVGNWPAFP
                     DSADALKRLKERFKLIILSNVDRGSFAKSNTKLGVDFDLIITAEDVGAYKPAQRNFDR
                     LFAEIGSIGISKNDLVHVAESLFHDHGPAQRNHLTSVWIHRRHEKGGSGATHPPEGVH
                     EPEWRFTSMATFADAALA"
     gene            1128..1868
                     /locus_tag="VHR64_15935"
     CDS             1128..1868
                     /locus_tag="VHR64_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304376.1"
                     /translation="MKRIALRSALASLSLCALLVLLAAGRLSAAAQDATPAALTVLPP
                     DASVGGQNLSQWHAQFWQWTFSLPSSVDPLADDNGERCVFGQHGPVFFLTISSTDVER
                     TCTVPDGATLFVPVMVVECSNVEKDPFYGANEADLTTCANAHIDVGLSEDLAKVSLTI
                     DGQAVGDLTPYRTATSLIQVPRPSNPETGEPGGVALSVGAGFAVLVGPLSDGEHTIEI
                     VTRDGVHITYHLTVSSGEATPTAGTPVG"
     gene            1929..2285
                     /locus_tag="VHR64_15940"
     CDS             1929..2285
                     /locus_tag="VHR64_15940"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX3304377.1"
                     /translation="MKEIIAPDGCDIREINERPAGERQEIVALFKALSDPTRFEIFRL
                     IAAQPDSICVCHITDRFDVSQPTISHHLKVLREAGLITVSRRGVWAYYAADPRGLAQL
                     RDSLELFAPPLLAAAD"
     gene            2349..2594
                     /locus_tag="VHR64_15945"
     CDS             2349..2594
                     /locus_tag="VHR64_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304378.1"
                     /translation="MVHADNALMNEFFRNAHCPDPRCAARFRPFAPAPAPVQHMDPPT
                     HRDRRLDARYKYGLVGMLTQPLRRLLPIYRVRLHHCC"
     gene            2661..3581
                     /gene="arsM"
                     /locus_tag="VHR64_15950"
     CDS             2661..3581
                     /gene="arsM"
                     /locus_tag="VHR64_15950"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX3304379.1"
                     /translation="MSTEQKPRSDAEVKELVRDRYASAAVRVIESSGCGPGCCGGSSI
                     DPVSVDLYDAGEEAELPTAAIQASLGCGNPTALAKLNPGEVVLDLGSGGGIDVLLSAR
                     RVGPTGFAYGLDMTDEMLALAEKNKAEAGATNVEFLKGNIEDIPLPDNTVDVVISNCV
                     INLATDKGQVLQEAYRVLKPGGRFAVSDVVSQGELPADLRQDMEAWVGCIAGALEENE
                     YRRLLSEAGFRNIEIQVTRVYDPRELAESLASGASCCGGDSPTWDESAYARFDASGGR
                     VVSAFVRAEKSRGVEKSRSREAGGLETSDL"
     gene            3581..3994
                     /locus_tag="VHR64_15955"
     CDS             3581..3994
                     /locus_tag="VHR64_15955"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX3304380.1"
                     /translation="MRRVLFLCTHNSARSQMAEALLRALGETAFESFSAGTVATSVRP
                     EAIAVMREVGIDISGQQSKTLGRYLDEPFDEVITVCDDANESCPIFPGARSRRHWSIP
                     DPSRVTGSDEERLAAFRSARDELQARIQEEILAPL"
     gene            complement(4069..4626)
                     /locus_tag="VHR64_15960"
     CDS             complement(4069..4626)
                     /locus_tag="VHR64_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304381.1"
                     /translation="MSDPRSNSGDDVSRFEPLPNTMGGADVGDVGDTSARPGAERSDN
                     SGTSAPGSGAKDKAKDTVYKAKDTIVGGVQQKASAVAGQAGAKASQIGDQATQKADMS
                     KDKAASGLDSLAHTLRDKSQTMGDGQVGSMATKAADKLEFGAEKLRSKDTDQFITDLE
                     ALIRKRPIESVVVAAALGFMFARAL"
     gene            4831..>5251
                     /locus_tag="VHR64_15965"
     CDS             4831..>5251
                     /locus_tag="VHR64_15965"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3304382.1"
                     /translation="MARANLIVVNGLSGTGKSALARHIARDLGYPFFGNDQLKEVLFD
                     TLGWSDLEWSKRLGTSASALLWSVARSMLEAGQSLVVEGAFHAGEDGPRLVGLREQFR
                     PRIAEIHCFASRDVLARRVKERAESGDRHPGHGDQDME"
BASE COUNT          910 a         1597 c         1713 g         1031 t
ORIGIN      
        1 ccgacaggcg tgcgcataat catcatcccg gtaatcgaga taatggtgcc ctcggtagtg
       61 gtaatggtgg cgatgcgagt cgtcaacatg gccaaatgat agcgacggat ccctgattcc
      121 gcaatccgcc tttcggcgga aggggacgaa tgggcgggat caagccaggg cggcgtcggc
      181 gaacgttgcc atggaggtga agcgccactc cggttcatgg acaccttccg gcgggtgcgt
      241 cgcgcccgat ccccccttct catggcggcg gtgaatccag acactggtca ggtggttacg
      301 ctgggccggg ccgtgatcgt ggaaaagcga ctcggcgacg tggaccaggt cgttcttgct
      361 gatcccgatc gagccgatct cggcaaagag gcgatcgaag ttacgctggg ctggcttgta
      421 ggcaccgacg tcctcagcgg tgatgatcag gtcgaagtcg acgccaagct tcgtgttgct
      481 cttggcgaag gatccgcgat cgacatttga gaggatgatc agcttgaacc gctccttgag
      541 ccgcttgagg gcgtcggccg agtcagggaa ggctggccag ttgccgaccg atgctccgaa
      601 ggccgcgcac tcctcgtcgg tggcgtcgac attgagattg gtggccatcg ttcgcagcgt
      661 ttcggcgagg acgagcggat agcggagatc cggatggcgc gtctcgatga tcgtctcgac
      721 gccgccgaac tgggccagca attcctcgcg gtcgatctcc aggccggtcc gctcgaccca
      781 tggctgaaga gcctcccaga tcccgctctc ccaatcgatc agggtgccgt agcaatcgaa
      841 gctgagcgcc tgaaacccgg tcaggtccat gacgtgcctc cttcatgacg cgcgaatcga
      901 ccaatggctg gatatcgtaa accgccgcga tatcggaaag gagttgccgc cgcacggcgt
      961 actccgtcga tcgtacgccg gtttcattcc ggaaccattt agggtgctgg tcgattgacc
     1021 agggcggata tcggcataga ttgagggagt acgtccgatt tttctccctt cttccgtcgc
     1081 atggcgctgt aatccgccaa gcttggatga atccaggaag gtccgatatg aaacgaatcg
     1141 cgctccgttc ggcactcgca tccctctccc tctgcgcgtt gctcgtcttg ctggccgcgg
     1201 gcaggctcag cgcggccgcc caggacgcga cgccggccgc cctgacggtg ctgcctcccg
     1261 atgcgagcgt gggcgggcag aacctcagcc agtggcatgc ccagttctgg cagtggacat
     1321 tcagcctgcc gagctctgtc gatccattgg ccgacgacaa cggcgagcgc tgcgtctttg
     1381 gccagcacgg gccggtcttt ttcctgacga tctcgtcgac tgacgtggag cggacctgca
     1441 ccgtgccgga cggtgccacc ctgttcgtgc cggtgatggt cgtcgaatgc tcaaacgtcg
     1501 agaaggaccc gttctacggc gcgaacgagg ccgatctcac gacctgcgcg aacgctcata
     1561 tcgacgtcgg gttgtctgag gatttggcga aggtctcgct gacgattgat ggccaggctg
     1621 tcggtgatct cacgccgtat cgcacggcga cgtccctcat ccaggtgccg cggccatcca
     1681 atccggagac cggcgagccg ggcggagttg cgctctcggt tggcgccggg ttcgcggtac
     1741 tggttgggcc gctcagcgat ggcgagcaca cgatcgagat cgtcactcgc gatggcgtgc
     1801 acatcaccta ccacctgacc gtgtcttcgg gcgaggcgac gccgacggcg ggaacaccgg
     1861 tcggctagga gcggcaccca catcggatca tattgacaat catcgatatc agatatagat
     1921 tggtatctat gaaagagatc atcgctccag acggctgcga cattcgggag atcaacgagc
     1981 gccccgcggg cgagcggcag gagatcgtag cgctcttcaa ggcgctctcg gatccaacac
     2041 ggtttgagat cttccgactg attgcggccc agcccgattc gatctgcgtt tgccacatca
     2101 ccgatcgctt cgacgtcagc cagccgacca tctcgcacca cctcaaggtg ctgcgcgagg
     2161 ccggtctgat aactgtctcg cgacgtggcg tctgggcgta ctacgctgcc gatcctcgcg
     2221 gtctggcgca gcttcgcgac tcgcttgagc tattcgcgcc gccactgcta gcggcggctg
     2281 actaacaagc tttttgtggc gcttatatat agatgatcgt cgatgtcatg gagaaaagga
     2341 gagttccgat ggtccacgcc gacaacgccc tgatgaacga gtttttccgc aacgcccact
     2401 gcccggatcc gcgctgcgcg gctcgttttc ggccgttcgc gccggcacca gcgccagtgc
     2461 agcacatgga cccgccaacg cacagggatc gccggctcga tgccaggtac aagtatggat
     2521 tggttggcat gctgacccag ccactccgtc gcctgctgcc aatctaccgg gtcaggctgc
     2581 atcactgctg ctaattgacg gcaccacgcg acgcgagcgg ttcgcgtcga aggcaaactc
     2641 actgaaggga gttctccatc atgagcactg aacagaagcc ccgatccgat gccgaagtca
     2701 aggagctggt ccgggaccgg tacgcctcgg cggccgtccg ggtgatcgaa tccagcggct
     2761 gcggccccgg ctgttgcggt ggatccagca tcgacccggt gtcggtcgat ctctatgatg
     2821 cgggcgagga ggccgagctt ccgaccgcgg caatccaggc gtcgctcggc tgcggtaatc
     2881 cgaccgcgct ggccaagctc aaccccggcg aggtggtgct cgatctcggt tccggcggcg
     2941 gcatcgatgt cctgctttcg gcgcgccggg tcggaccaac cggattcgcg tacggtctag
     3001 atatgaccga cgagatgctg gccctggcgg agaagaacaa ggctgaggca ggtgccacca
     3061 atgttgagtt cctgaagggc aacatcgagg acatccccct gccggataac acggtcgatg
     3121 tggtcatctc gaactgtgtc atcaacctgg cgaccgacaa gggacaggtt ctgcaggagg
     3181 cgtaccgcgt cctcaagccg ggtgggcgat ttgcggtctc ggatgtcgtc tcgcagggcg
     3241 agctgccggc cgacctcagg caggacatgg aggcgtgggt cggctgcatt gccggagcgc
     3301 tggaggagaa cgagtatcgt cgcctactga gcgaggctgg gttcaggaat atcgagatcc
     3361 aggtcacgcg cgtctacgat ccgcgcgagc tggccgaaag cctggccagc ggcgcgagct
     3421 gctgcggtgg cgattcccca acctgggacg agtccgcata cgctcgcttc gatgccagcg
     3481 gcggcagggt cgtcagcgcc ttcgtgcgag cggagaagag tcgaggagtc gagaagtcga
     3541 gaagtcgaga agccggagga ctggagacca gcgatttgtg atgcgccggg tgctctttct
     3601 ctgcacgcac aatagcgccc ggtcgcagat ggcggaggcg ttgctccggg cactcggcga
     3661 gactgcgttc gaatctttca gcgcggggac cgtcgcgacc tccgtccggc cggaagcgat
     3721 cgcggtcatg cgcgaggtcg ggatcgatat cagcggccag cagtcgaaga cgctcgggcg
     3781 gtatctcgat gagccgttcg acgaggttat taccgtctgc gacgacgcca acgagtcctg
     3841 cccgatcttc ccgggcgcgc gcagccgccg ccactggtcg attccggatc cgtcgagggt
     3901 cacgggtagt gatgaggagc ggcttgcggc gttccgatcg gcgcgggacg aactgcaggc
     3961 gcgtattcaa gaggagattc tcgctcctct atagctatga gatcagccgc actccgggcg
     4021 cctcgaggcg cccggtgctt atcgccctcg atgagtcaag ctcgacgctc agagtgcccg
     4081 ggcgaacatg aatccgagcg ccgcggcgac tacgacggac tcgatcggtc gcttgcgtat
     4141 cagcgcttcg aggtcggtga tgaactgatc ggtatctttc gatcgcagct tctcggctcc
     4201 gaactcgagc ttgtcggcag ccttggttgc catgctcccg acctggccat cgcccatggt
     4261 ctggctcttg tcccgcaagg tgtgcgccag ggaatcgagc ccgctggccg ccttgtcctt
     4321 gctcatgtca gccttctggg tcgcttgatc gccgatctgg ctcgccttcg ccccggcctg
     4381 accggccact gcgctggcct tttgctggac tccgccaacg atcgtgtcct tggccttgta
     4441 gaccgtgtcc ttggccttgt ctttggctcc agacccgggc gcggaagtgc cgctgttgtc
     4501 gctgcgttct gcgccgggcc ttgcggacgt atcgccgaca tctccgacgt ccgcgccgcc
     4561 catcgtgttg ggcagtggtt cgaaccggct gacgtcatcg ccggagtttg accgcgggtc
     4621 actcatcttt cgactccttc gcatgcacca cggagcaagg ctcgccgttt atttgcgata
     4681 agactcaatt ttaggcacag atagtgttgc ctgtgcaact gtcggctaca gcgtcgcgcg
     4741 cgccgctgac cgcccggcac gcgtctggta tttcattgct ggaccgcggt caagcgggat
     4801 cagttcgttg atattgggga catgacctgc atggcgcgcg ccaatctgat cgtggtgaat
     4861 ggcctttcag ggaccggcaa gagtgccctg gcccggcaca ttgccaggga tctcggatac
     4921 cccttcttcg ggaacgacca gctcaaggag gtgctcttcg acacgctcgg ttggagtgat
     4981 ctggagtggt cgaagcggtt gggtaccagc gcttccgcgc tgctatggtc cgttgcccgg
     5041 agcatgctcg aggcggggca atcgctggtg gtcgagggcg catttcacgc gggcgaggac
     5101 ggaccgcggt tggttgggct gcgcgagcag ttccggccgc ggattgccga gatccactgt
     5161 ttcgccagcc gggatgtgct ggcccgacgg gtcaaggagc gcgcggagtc gggcgatcga
     5221 catcccggtc atggcgacca ggacatggag g
//
LOCUS       DATAOZ010000640        25117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4721448, whole genome shotgun sequence.
ACCESSION   DATAOZ010000640 DATAOZ010000000
VERSION     DATAOZ010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 25117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25117
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4721448"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VHR64_15970"
     CDS             <1..453
                     /locus_tag="VHR64_15970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX3304383.1"
                     /translation="HAGTIMVERVRLGEIFAVARLQRRSFRRSLAYRPTTLLMLYLWP
                     RASFLVARAGREIVGCVIGDVHGGQSRVISICVDPDWRRRAIGTRLLSEIERRLPEGN
                     MILMVEAGNTGAQQMYRDCGYLPVGESRDYYGRGRNGIWMQKTRPLRT"
     gene            complement(823..1095)
                     /locus_tag="VHR64_15975"
     CDS             complement(823..1095)
                     /locus_tag="VHR64_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304384.1"
                     /translation="MHWIPPEMNPERLLTIILAIAFLAAPPIALLYGPGPGLFVLAAA
                     LLATIMLAWSVRAHVPPHVQQRLQAMLVLNGVLLVAAVVALVLVAL"
     gene            1171..1971
                     /locus_tag="VHR64_15980"
     CDS             1171..1971
                     /locus_tag="VHR64_15980"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3304385.1"
                     /translation="MTQPVEWNAEHYHVVSRPHEVWGARVLDRVPREGIGVAIDAGCG
                     TGKITRELLDRLPEATVYALDVSSSMLAVAERELAPVYGDRVRFQQADLTTVTGSEIG
                     TTADLIFSTATFHWVKDHDQLFPRLFDLLNPGGSLIAQCGGGPNIATLCDRMAELMAS
                     PQFAEYFDGWTDPWYFASAEETANRLKATGFTDVWTAVEPHPAPMADADEYRSFITTV
                     VIRDQLARLPDQARKDTFVDELTRRAATDDPPYELDYWRLNMTAKRSA"
     gene            complement(2203..3498)
                     /locus_tag="VHR64_15985"
     CDS             complement(2203..3498)
                     /locus_tag="VHR64_15985"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX3304386.1"
                     /translation="MAFMGETSDVGVAELLSVLARRGHTGRLHINSQGEEVQVILNGG
                     KVTQVSSSHHSLRIGRVLVRMGALKEEELNEAVKQQVSQQGNRPLGQILTASGYVTQS
                     DLARAAEEQATDALSRVFGAISGTFYFTGLEAGKTRPGLMALNAEGIVLEASRRADEI
                     EALRRMMPAEDAVVVLDRERMPIGATVPEHEQRVVRLLGLGPLTIGELIAELGDDERA
                     VLRAIVGLQERGIVSSRLPEHAGEIPALHEAAVTPRKVAELRDLIGSHLSLDPTGGVP
                     GIDQIRAAIPAGTQTVARATRVIRDVVGAFNAGLPLLAFAHFTDDYFRRLAPIADDEL
                     ALLDRIGEDQVVDEHQTFIELRDVRTLADGRISGIAVVSLPDDMPTQRVVIFADVRDR
                     VQIDAIVEPGHQRERTTQTTLLRPAALLGADHTTLRRAL"
     gene            3771..4532
                     /locus_tag="VHR64_15990"
     CDS             3771..4532
                     /locus_tag="VHR64_15990"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3304387.1"
                     /translation="MTVRSDAEHDDRPARLADINSSEPSGDGKWLSIHEACRLLGVDQ
                     STLRRWSDSGKVPVFRTPGGHRRYAESELLALVGNGSRRFERQRVMARQLADQSRAGF
                     VDEFWRTARTRRWYRAYSPGQLEDLRRLGRRLIDLAVRYASTAAGPERQSLIDEGRSI
                     GHQYARVGAMAGLSGAEAVEAFLYFRAPVVHSLTSLTEGARMGAPAMLRVSAEVNSFL
                     DQVLLAMVHEHEEQSRLTAGATLQPSLDGQILDDA"
     gene            4793..5320
                     /locus_tag="VHR64_15995"
     CDS             4793..5320
                     /locus_tag="VHR64_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304388.1"
                     /translation="MRRLIVALILLGSVLALVPAAGTTAAPAGTYYVMPTLAEITTIC
                     TGVDCDTFTARYDYTGAASCTGCNPTDPFRVGDFSLTLTGTRFLPSDPYRAKSGTGTL
                     TVVWADSTTTQTSYTFKARDSKTYALTGAVTGGTSSRFATGTALDGLVGVPPNPVVPP
                     NPVEPGITSGTVRFG"
     gene            complement(6558..8894)
                     /locus_tag="VHR64_16000"
     CDS             complement(6558..8894)
                     /locus_tag="VHR64_16000"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3304389.1"
                     /translation="MSSAPEARTTLEPTPIRPSTGRQHLTALPRPLTSFIGREHETEA
                     VVALLADPRIRLVTLTGPGGVGKTRLSIHLAERVASGYPSGVWFVSLASIQDSELVAG
                     AIAQVVAPPNAGQQTAEEQITAALQAGRALLILDNFEHLLGAAPLVGRLLTACPMLTI
                     LATSRAHLQVSGEHAFAVPPLDVPDAGSGSLGEHDAVRLFAERATNTSLVPDSKRFDV
                     IAAICRRLDGLPLAIELAAAWVGVLPLTQLLDRLNARLPMLTGGRCDSPTRHQTMRDS
                     IAWSYDLLSPDEQSVFRRASVFVGGFCLEAIEEVAADAVDRAEVLGHVRSLVEKSLIR
                     PVPVADVPRYLMLETIREFAMEQLDQAGEVEETRAAHGEWCAGFAERFSIEVTGPDLA
                     KWTAWGEAELDNVRLALDHADARGDAGSAVRLAGGIGWLWSPGRLQEGRARLERVLSM
                     PGTDPSPRCFATGLLCAGGIEHWLNEYDAATAWYIRAEEVCRASGDGTQLAIALRGIG
                     SVAIDTGDPETATRLLTESRKRSREIGSAWDVAYTTHLLGITAFVSGDIETAFDCHGE
                     ALAEFRGLSDHGYVLTSLEPYGRTALLLGKRKVARNAFLEMLELSGGDSWNTARAIRG
                     LGVVAAAEGDARRAARLMGAATRVLDAIGTPERPSIGDYYAQLRDEVRRSLGEEAFGL
                     EWEAGSALTLEMAIEDARRLRAELSGTSVSDGEAELGLTRREIEVLRLVREGLTDREI
                     SERLYISRRTVSKHVESILSKLNVDSRRAAALTVLANT"
     gene            9028..9897
                     /locus_tag="VHR64_16005"
     CDS             9028..9897
                     /locus_tag="VHR64_16005"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX3304390.1"
                     /translation="MEQSLAHSHRDVLRGYLVLPHAVPVIVVLAATAAFGLIAASGWP
                     GLGPMLRLLGAMLGAQLAIGAVNELADADLDAIAKPDKPIPGGLVSRRGAGIVAGCGI
                     GMMAALSLTFGVASFLLCTIGTLVGIAYSLWFKRSIWSWVPYLIALPLLPIWVWTALD
                     TLPTALLAIYPIGAAAIIAVQIAQSLPDVTVDQETNVRTLATVLGPNLARNVCWIALL
                     VAVGLAAICAPWLTDRPGFVWLAGLIAVGLIGVNVAIWSRDARRGVLAAFPCVACAAV
                     ILGLGWAAALAAG"
     gene            complement(10037..11482)
                     /locus_tag="VHR64_16010"
     CDS             complement(10037..11482)
                     /locus_tag="VHR64_16010"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX3304391.1"
                     /translation="MAELELFSPSARTALQFARAAAERMNHPSIGTEHLLLGVVEERE
                     DLAGYLLGLLGVRLKDVTRAIERMVDRSDGPLLRSLDLTAHARLALETAIQERRRLGH
                     DQIQTVHLLLGILGMPTSAAAGIFKQLAVDVDELRSSLYSMYASDGDRRPIGPVLERI
                     RDERRAHDDLERAQAYANLSEDDVGGRARTILRFARNIAHSLGHETVGTDHLLLGFVA
                     DRDCEAARALSGLGLSEKRLTLAVRFARGVSNQPQSDIVRPIPLSPRAWNAIELALIS
                     ARRRHARAKVAGQVRYKWWPHEPCPVDSADLLLGVIEAGTGSAVTILTGFGLDLALVR
                     QRVLALRGSTFGSVRRDGPAPGTFWEWLNQQLIWRQWSLADLAREARVDRRRLRAWVR
                     GDQRPSAASCDVLAAACMADPNLVRRLAGRPLKPAAVAKILPPEPPADRTEAVEAMRI
                     LFERVRWDEGRINRMTTLLRLMAENDEDAAD"
     gene            11673..11861
                     /locus_tag="VHR64_16015"
     CDS             11673..11861
                     /locus_tag="VHR64_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304392.1"
                     /translation="MSGSEILLVALAILVPLAIAIAVTLWTLQPAVLRNERAKRARKR
                     FAQAPPRKAAEEPGSHPE"
     gene            12047..15163
                     /locus_tag="VHR64_16020"
     CDS             12047..15163
                     /locus_tag="VHR64_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3304393.1"
                     /translation="MELKDLITGAQEAMDQGDFRLAIAAGEHALVGYDACLSAHRMLG
                     EAYLERGDQQTAIGHFERTLAIDPLNVVARLGLGVASEETRRYEDAYGHYLCAWETNP
                     ALDQVRDELVRLRGVLNVEGRLHPTRAGLAGIHARCGQFGRAAGEWRAVLSLEPDSSR
                     ARTALAELLWRQGDDAGASAAAREALRGCPENARALLILADVEKRRNGPTAADLIQRY
                     SQVDPAGEIVETMRHWRSDVDLDDLLAGAALIDTFDFESNRSRQTSGLSRKGITSGLA
                     TSQMAAPDLWDTLVQDLANGVPSKPAPTLATGVEPFSWADGAPPAELASPAAEPPAQP
                     VVATKPADSDIFPELASMDAFVVPDVEETAAPEVDQPVEPEDDLLSIFSRTDAAIPDV
                     SIPSADAAAPSDDFDFDLVPFSLDELTGVVAPSHEVDDAALAATFGVAADPLPEPPAA
                     PVAPVPPVIPEEPVVAAAAPEPYVDPFVSADGRIDLTAGWDTLDRVLEAATPGASGEG
                     GFDALIAELGVDGVVPFDGAVESADEDAWSPFSDVDFDVPVAGAPVRNEPFDIVIDPS
                     VPDATGETLDLSDVEALDINTFVAPEPEPVAEQRQEPALGSDILAGIPLPEPSGYTQL
                     LRNVDEETLPDHAGDDEIDPFANPDATGEPLAFDELIGVTSSDGTGPLAPIAAEPAEV
                     DLAAELDFSRTDLSSAVPQVDAEPFDFGIDGVEPFDTGGFDLAATIESESAYGQVRPF
                     SEPEPEPAVSDLPLAAELADIVPFAFDDGSLNGVDGSPVDFSELDVAPEPMLFAPPAV
                     AEPEPILFEEAEPAQFEYEAPAAELEVESPPADTWFDGEEPVEVAAELDEASVDDEPE
                     DLLVAEVDMAEPIEPILAPVGIRSVSWPQFVGQTSELIDRPVEGGSLFARIAAQKEAL
                     VEMGVVATGRRLIPIPQVTEPAVAVLEPVIAIAEPVVKPPEPPVAAAAEAEEQIRIDL
                     MGMRVRLIEDESSAREIADTLEEAIAGGLQSPLALRVLGEAYLKLGMVERAAAQFRQA
                     MLTRRRGG"
     gene            15175..15564
                     /locus_tag="VHR64_16025"
     CDS             15175..15564
                     /locus_tag="VHR64_16025"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX3304394.1"
                     /translation="MSAVLERTDQRTTSVDVILRTLLAYDEVVGAVAVNTEGLVMGSA
                     GMTETDVDVVSLLGASLTGVAERTTRRLGTGSAVGISLVTTDGMITIGNGGDFAVMVF
                     SARCDSVALLEALADPISRIRAIVDPT"
     gene            15693..16151
                     /gene="ndk"
                     /locus_tag="VHR64_16030"
     CDS             15693..16151
                     /gene="ndk"
                     /locus_tag="VHR64_16030"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX3304395.1"
                     /translation="MADTEQTLIIVKPDGVQRGLVGEIVGRLERRGLKIVAMQMQEVS
                     RETAERHYAEHEGKPFFAGLVDYITSAPVVVMVLEGPQAVAAARSTIGSTKPVEATPG
                     TIRGDLGMMVGRNLVHGSDSPESGKREIGIFFGDSATYGYARDMDRWILE"
     gene            complement(16233..16619)
                     /locus_tag="VHR64_16035"
     CDS             complement(16233..16619)
                     /locus_tag="VHR64_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304396.1"
                     /translation="MPVDQLSAIRDRFLPMLTFAQQEYGYRVPAGYPVIHDEVKRGVV
                     GIEIDPAHSVYVTTDGERLYADIAYRSTRYDTRSSASREKYAGQMVVDRRELPGDVSD
                     QELRNLIGEVMSRFNQQQTILYITDD"
     gene            complement(16702..17289)
                     /gene="dcd"
                     /locus_tag="VHR64_16040"
     CDS             complement(16702..17289)
                     /gene="dcd"
                     /locus_tag="VHR64_16040"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX3304397.1"
                     /translation="MILSDVDIRKALADGRIRITPEPDLDAQLGSVSVDFRLGTTFMV
                     FEHSRHSFIDPRSPQSIGDAMRTIEVAPEEPFIMQPGDFALASTVESLELPDNLLGRL
                     EGRSSIARLGITVHSTAAVFEPGWVGTATMELSNLGRMAVALYPGMRICAFSFEEVSS
                     PVSVPYRMKRNNKYAGQLTPRASQLSDEAPVGVER"
     gene            17471..19285
                     /locus_tag="VHR64_16045"
     CDS             17471..19285
                     /locus_tag="VHR64_16045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX3304398.1"
                     /translation="MADSATPSYFQRVHRRAQHRATTVEAARISASRQPNVGQRRYKQ
                     IKQSAPQADTPAPPTPPRQSTLVKTTPIASLGSELAQLKARQEKHHGPKWWSGIASHP
                     VRSAFLAVIILSLSGVLAIAGPIAYRSYRAYQDVFVTQVPDQESAFHAEINPEGTPVL
                     VANQAQESASIPEWDGTSRLTLLLLGVDKRENDYARSDTIILVNIDPVAKTARMLSIP
                     RDLKVIIPGFGAHKINAAYAFGDANKNVPGGGPGLMMRTIETNFGINIDHFAEVDFNG
                     FVKIVDTVGGVTLDVPYPIRDNEYPAPNNQYMRIFFPSGWQHMDGQRVLQYARTRHDD
                     GDGRRSARQQQVLLALRQQALDLNLLKQAPTLISEVGDTVRTDLSSGQALQLARLATE
                     INQSEIQQLTLDPALITWQTDEFYFQGDWAMIGDIMTEFTGQKIVPPMSALANPSYET
                     TISILDGTLIPGLGPRVLDVVKSNGFADSTTVDLPDAGNYPETKIIVSPDNIATAYLL
                     AGILGVPETAITASDDIQTPTIVATNTATATSAAAPVGTPTAGTPTADGKLDVVPLFP
                     TAVPESGKASPAPAGEIIIILGDDLPDPAWYNVDPNAQ"
     gene            complement(19453..20538)
                     /locus_tag="VHR64_16050"
     CDS             complement(19453..20538)
                     /locus_tag="VHR64_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304399.1"
                     /translation="MSVKTSRRAVLASALAAALISRGVSAQKLGKIADDLALAHFPAL
                     PDDQGAWPTSIVAWADIAGYCEAAGIEKPNSFGEETVKVWSQIGQRIPMNDDTLLLFM
                     NAPWGEIVGFEPWDIDQLAVAFDPPDQLRIYSGAFDPERVEQAFAQWSYSASTNGDFR
                     VLVNPEDGLDPSRDLDRYALGRFNHVGVTESLVIASRSAEVLAAAIDVAANEAQSIAD
                     GELFADVAKVIEGMHGYVLLNGALLGSPTVNPGGLVDGETIPPAHLFLGGIEAKKDGQ
                     LVSLIADLGDVADAKSAVTVVQDRIDTLTSPISKRPYTEDLEGYEVDVVEGTGLLRLT
                     VPNDTFAMRWMRYIFAGDLLFLATKAS"
     gene            complement(20593..21582)
                     /locus_tag="VHR64_16055"
     CDS             complement(20593..21582)
                     /locus_tag="VHR64_16055"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX3304400.1"
                     /translation="MARVYDELTIFGGNSNPALTDQVCAYLGLPRGRAEVFKFSNDNT
                     FVRIGESVRENDVFVIQSFAEPVNDSIMELLIMIDTLRRASAGRITAVIPYFGYGRTD
                     KKDQPRVPITACLVAKLVGVSGADRVLAIDLHAGQIQGFFDIPVDEMSAMFLTANYFV
                     DKRLENPVVVSPDLGNTKRARNFAQILDAPLAIIEKRRIGNQDRSQVLNLIGSVAGCQ
                     VVIVDDEIDTAGSITQAAQVCMEEGATEVYATCVHPVLSGPAVERLQKSVIREIVVTD
                     TIQVPQHKRIDKLTMLSVAPLLGETIQRIHTGTSVGAAYKAASGAMTLRMQLY"
     gene            complement(21575..22417)
                     /gene="rnc"
                     /locus_tag="VHR64_16060"
     CDS             complement(21575..22417)
                     /gene="rnc"
                     /locus_tag="VHR64_16060"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX3304401.1"
                     /translation="MSRRAKPQTIESTAGEAEDNATRFAASLGLAFHDLDVLRLALTH
                     RSVAHELRSISPELELPPSQRSNERLEFVGDAVIGYIVAADLYRRFPEAPEGDLTARR
                     VALVRSERLVAWARSLKLGNYVYLATGERVSDRDRDRILSGAFEAVVGAITLDQGIEA
                     ARAFMEPFLDADAFAALEDQIAANPKGQLQEYVQDHYRQPPIYRIIGEEGPDHARTFT
                     AEVLIGDSVLGSGTGESKRLAEQAAATEALERVGELHKRRATRSVRKRRKPSAPKGGT
                     ELDG"
     regulatory      22561..22667
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22719..23726
                     /locus_tag="VHR64_16065"
     CDS             22719..23726
                     /locus_tag="VHR64_16065"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3304402.1"
                     /translation="MPLTRRSFLAGSLGAAAAFGTASPGRAQGPAKASIALDWYPNAN
                     HAGLFWAQAKNLFSDAGIDITIQTPADPTTVLQTVAAGRDTFGISYQPDILLARAQDV
                     PVVAVCAIVPRPLQGVMSLKSAGIARPADLRGKTVGYPGIPSQEAFLKTMLAADGVAM
                     DQITLNNVEFNLLPTLISGQAAAVMGAFWTHETIVAELEGYPVEMMKVEDWGVPIYDE
                     LVLVTSEETLANKRELVTGVLEVVRAGYLAAAADQKQAVDILVAAYPETERPVEEQGI
                     ALLADLWTQPVPGFGIMDPDVWAKFADWMIEHELLPAGFELNNTIGANLPAQPLATPT
                     R"
     gene            23989..24576
                     /locus_tag="VHR64_16070"
     CDS             23989..24576
                     /locus_tag="VHR64_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304403.1"
                     /translation="MSDDSPFPSRSWLPTLNAGDRFTTATETKTATVEPVAPVDEAAP
                     EPAERNRNHLLAQDWSSALESQFGLTAPVVKVEPEESIAALKERLAYYEHFDDLIRDN
                     IARSSALFEAVFAERDKARKVQSDAESTMSAVAAEADRRVAAERQHVQHILMSLMDEA
                     TFLHQRSDALIQRLAEALTEMAPTSNEADEPVSGA"
     gene            complement(24828..>25117)
                     /locus_tag="VHR64_16075"
     CDS             complement(24828..>25117)
                     /locus_tag="VHR64_16075"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304404.1"
                     /translation="GPVTACGMASAGDRGVQRVEVSIDDGATWADAQLEPALNMPFTW
                     VRWAFPFTAQSGQLKMKIRVTDGQGEVMKEDEQAPLPDGATGWPSRTINVK"
BASE COUNT         4391 a         8416 c         7991 g         4319 t
ORIGIN      
        1 catgcgggca cgatcatggt cgaacgggtc aggctcggtg agatcttcgc cgtcgccagg
       61 ctccagcgcc gcagtttccg aagatcgctc gcctaccggc cgacgacgct tctcatgctc
      121 tacctctggc cccgggccag cttcctggtc gcccgggccg gacgggaaat cgtcggctgt
      181 gtgatcggtg acgtccatgg cggccagtcc cgcgtgatca gcatctgtgt cgatccggac
      241 tggcgtcgac gcgcgatcgg gacccgactg ctgagcgaaa tcgagcgccg cctgcccgag
      301 ggcaacatga tcctgatggt cgaggcgggc aacaccggcg cgcagcagat gtaccgggac
      361 tgcgggtacc tgccagtcgg cgagagccgg gattattacg gccgcggccg caacggcatc
      421 tggatgcaaa aaacccgccc gctccgaacc taagatcgcc acagagtaat gtggcgcgga
      481 tgcctctcca cgctctccta ctctccctga tggggcgaga gcatcccgcg atagccggaa
      541 cggccatgtg ggttgggccg gagatctggc gtgtgtcctc gaacgggctc ccggcgccgc
      601 ccggtgcgaa aggcccgggc taaatcacga taagtccact aaagggactg actaaaagag
      661 tcggcttcag ccgccttatt caggatcagc cccggccttc agtccggggc gatgccgagc
      721 gagtattgcc gaactccaac caccccgtcg gccacccctt catcgccctg cgggccaccg
      781 ccgggcacag aggcgccggc gccacgttga agttgacgcc gtctatagcg ccacgagcac
      841 cagtgcgacg acggctgcca caagcaggac gccgttcagc acgagcatgg cctgcaaccg
      901 ctgctggacg tgtggcggca catgcgcgcg gacggaccag gccagcataa tcgtcgccag
      961 cagcgccgcg gccaggacga acaggcctgg tcccggacca tagagcaggg caatcggcgg
     1021 cgcggccagg aatgcgatcg cgaggatgat cgtcaacagg cgctccgggt tcatttcggg
     1081 cgggatccag tgcatcggcg gctcatccgg gtaggatacc gaagcgccgg cacgagacgc
     1141 ggcgcgattg ccgcctgcac gaggatcgcc atgacgcaac cggtcgaatg gaacgctgag
     1201 cactaccacg tcgtctcccg accacacgag gtctggggag ctcgcgtgct cgatcgcgtc
     1261 ccgcgcgagg gcatcggcgt cgcgattgac gccggctgcg gcaccggtaa gatcacccgg
     1321 gagctgctcg atcgcctgcc ggaggcgacc gtctacgcgc tcgatgtctc gtcctcgatg
     1381 ctcgccgtcg ccgagcggga gctggcgcct gtctatggtg atcgggtgcg attccaacag
     1441 gcggatctca ccacggttac cgggtccgag atcgggacga cggccgacct gatcttcagc
     1501 accgcgacct tccactgggt gaaggatcac gatcagctct ttccccggtt gttcgatctg
     1561 ctcaacccgg gtggctcgtt gatcgcgcag tgcggcggcg gtccgaacat cgccaccctg
     1621 tgcgaccgta tggccgagct gatggcctcg ccgcagttcg ctgagtattt cgatggctgg
     1681 acggacccgt ggtatttcgc gtcagccgag gagacggcca accgactgaa ggcgaccggc
     1741 ttcacggacg tctggaccgc cgtcgagccg cacccggcgc cgatggcgga tgccgacgag
     1801 taccgctcgt tcatcacgac ggtcgtgatc cgggatcagc tcgcccgcct gcccgatcag
     1861 gcgcgcaagg acaccttcgt cgacgagctc acccgccggg ccgcaaccga cgacccgccc
     1921 tacgagctcg attactggcg cctcaacatg accgccaaac gctcggcatg acctaaccct
     1981 gcgttggccg cgatccctcc tcgtccgtag tggcggggct ccgtacccgc ctggacctga
     2041 tcagtgcgca cgttcgcgtg taccacaagt cgcacggatg tataccggtg aggtggacga
     2101 tgcatggtcg ggagcggctg ggcgagacaa gacgggtacg gagccccgcc actacggaat
     2161 agacaagggg ccggcgcctc gtggagaaac ttggtccgcc ggttacaggg cgcggcggag
     2221 ggtggtgtgg tcggcgccga ggagggcggc cgggcgcagg agcgtcgtct gcgtcgtgcg
     2281 ctcgcgctgg tggcccggtt cgacgatcgc atcgatctgc acccggtccc ggacatcggc
     2341 aaagatgacg acgcgctgcg tcggcatgtc gtccggcagg ctgacgaccg cgattccgct
     2401 gattcgcccg tcggccagcg tccgcacgtc gcgcagctct atgaaggttt ggtgctcgtc
     2461 gacgacctga tcctcgccta tccggtcgag cagcgcgagc tcatcgtccg ctatcggcgc
     2521 aagccggcgg aagtagtcgt ccgtgaaatg cgcgaaggca agcagcggca atccagcatt
     2581 gaacgcgccg acgacatccc ggatgacccg ggtcgccctg gccacggtct gggtgccggc
     2641 cggaatggcc gcgcggatct ggtcgatgcc cggcacgccg ccggtcgggt cgagactgag
     2701 atgcgacccg atcaagtctc gaagctccgc aaccttgcgt ggcgtgactg cggcttcgtg
     2761 cagcgccgga atctcgcccg catgctctgg aagtcgtgat gagacgatgc cgcgctcctg
     2821 aagccccaca atcgcgcgca ggactgctcg ctcatcgtcg cccagctccg caatcagctc
     2881 gccaatcgtg agcgggccca ggccgagcaa ccggaccact cgttgctcgt gctccggcac
     2941 cgtggcgcca atcggcatcc gctcgcgatc gagcacgacg acggcatcct cggccggcat
     3001 catccggcgc agcgcctcga tctcgtccgc ccgccgcgac gcctcgagaa caatcccttc
     3061 ggcattcagc gccatcaatc cgggccgcgt ttttcctgcc tccagaccgg tgaagtagaa
     3121 ggtgccggaa atcgcgccga agacgcggga gagcgcgtcg gtcgcctgct cttcggccgc
     3181 gcgggcgaga tccgactgcg tgacgtaccc gctcgcggtc aggatctggc cgagcggtcg
     3241 atttccctgc tggctgacct gctgcttgac cgcctcgttc agctcctctt ccttgagcgc
     3301 gcccatccgg accagtactc ggccaatccg caggctgtga tgcgaggagg agacctgggt
     3361 gaccttgccg ccgttcagga tgacctgcac ctcctcgccc tgcgagttga tatgcagtcg
     3421 accggtgtgg ccgcggcgcg ccagtacgga aaggagctcc gccacaccga cgtccgatgt
     3481 ttcacccata aacgccatgc aaacagacac ctcatcgttg acaatggatc acggcagccg
     3541 tggcgacagg ttccgctctc cataacatga agcgacgctg gtcctgtgcg ccatgccccg
     3601 ggagtcctaa ccggtagatt taggcacatt tgtgcgcgcc gggctgtcgc gcggttggcg
     3661 ttgacaggtt gtcccggccg ggtagacttt ggttctcaac ggtgcgctga tgtcggcctc
     3721 gcattccgcg gctgcgccca ctgtgcccga cacaccttgg gggagaaaga atgaccgtgc
     3781 gttccgatgc tgagcacgac gaccggcccg cccgcctcgc ggacataaac agttccgagc
     3841 catcgggcga cggcaaatgg ctttccatcc atgaagcctg tcgactgctc ggcgtggacc
     3901 agtccacgct gcgtcggtgg agcgattcgg gcaaggtgcc ggtgttccgg acgccgggcg
     3961 ggcaccgtcg ctatgctgaa tccgaattgc tggcgctggt cggcaacgga tcgcgccggt
     4021 tcgagcggca gcgggtgatg gcgcggcaac tggccgatca gtcccgcgcc ggttttgtcg
     4081 acgagttctg gcggacagcg cggacgcgtc gctggtatcg cgcctactcc ccgggccagc
     4141 tcgaagatct ccgccgcctt ggtcgccgcc tgatcgatct tgccgtccgc tatgcatcaa
     4201 cggcagccgg accggagcgc cagtcgttga tcgatgaggg ccgctcgatc ggtcaccagt
     4261 acgcccgcgt cggcgcgatg gccggcctga gcggggccga ggccgtcgaa gcgttcctct
     4321 atttccgggc gccggtcgtc cactccctga cgtcgctgac cgagggagcg cggatgggcg
     4381 cgccggccat gctgcgggtc tcggccgagg tcaacagctt cctcgaccag gtcctgttgg
     4441 cgatggtcca cgagcacgag gagcaaagcc gcctcaccgc cggcgccacg ctccagcctt
     4501 cgctcgacgg ccaaatcctg gacgacgcgt aggcagactt cagacttcag aaatgagcat
     4561 ggcatatagc atgccgtgct gctcctctat tgtctgaccc ctgatcactg cccctgatcc
     4621 gtcaactgac gtctgaagtc cgacgtctga cgactcaaaa atacgtatct cccctcgcaa
     4681 aactacgtac atcctcccca tgcgcctgga actcgcgctc ggcatgatcg gcggcaatcg
     4741 cgccacatca aacgagtatc gagtctgccg agggtgcgga ggaggatgcg tcatgcgacg
     4801 actgatcgtt gccctgattc ttctgggaag cgtgttggca ctggttcctg cggccggcac
     4861 caccgccgca ccggccggga cctactacgt gatgccgacg ctggccgaga ttacgaccat
     4921 ttgcaccggc gtcgactgcg ataccttcac cgcccggtat gactacactg gcgcagcgag
     4981 ctgcaccggc tgtaacccga ccgacccctt ccgcgtcggc gatttcagcc tgaccctcac
     5041 tggcacccgg ttcctcccca gtgatcccta ccgggccaag agcggcaccg gcacgctgac
     5101 cgtggtctgg gccgatagca cgaccaccca gaccagctac accttcaagg cccgcgactc
     5161 caagacctac gccctgaccg gcgcggtcac cggcgggacc agctcccgct ttgccaccgg
     5221 cacggcgctg gatgggctgg tcggcgtgcc gccgaatccg gtcgtgccac cgaatcccgt
     5281 cgagcccggc atcacgtccg gaactgtccg cttcggctag tcgactctcc ccggccggca
     5341 tcgcacggtc gtcgccggtc gggaccattc ctgcacgacc tgaacggcgc caccctcggc
     5401 gccggccggg tctctcgctt ccagatcgac cgttgttggt tcggggagca catctgctcc
     5461 acgagaaagg accgaacgcg tggacgagtc caagttcgac gatctcaccc gcttcatggc
     5521 cgaaacgccg tcccggcgca cggtcgtcaa ggcgctggta ggcgccattg ccgggagcct
     5581 cgcttcggtc gggaccgcgc tcggcgcgcc acgctgcaag cgggtcaacc aggcctgcca
     5641 gaccacggcc gattgctgcc ccggcccgac tgccaacggc aatgtctact gcaagcaggt
     5701 caagaaaagc acaaaaatct gcgcgacttg cccacccgag acgccgaccg cctgcaacgg
     5761 aggctgcgtc gataccagca ccgacagcaa gaactgcggc acctgcggca acgtctgcct
     5821 tggcggaacg acctgcgtca acggctcctg ccggtgccca gccggccagc acctctgcgc
     5881 cgacggctcc tgcaaggaat gttgcgcgga cagtcattgt gatggcggag ccgaacgatg
     5941 ctgcaacgga atctgccgaa acctggatgg cagcaacgag aactgtggtg catgcggaca
     6001 tgtctgcccc gcaggcacag actgcatgaa cgggtcgtgt gtctgcctga atggccagca
     6061 tctctgcgcc gatggcagct gccgcgagtg ctgcacgttt gagcattgtg tccccatcat
     6121 cgggccaggc gaaggagcga tctgctgcga cggcacgtgc aaggcgagcc gcttcaacca
     6181 gaacaattgc ggtgggtgcg ggattgtctg cgccgctgac caagtctgcg ttgatggcga
     6241 ctgcacgtgc cccaacggtc aggcgccacg gccggacggc acatgcacgt gcgggggggc
     6301 cgaatgttca gcggccgtcc cttgctgcga cggctattac tgcaactttg gggtatgcct
     6361 ggcttgcgaa ccaattggag gcagctgcga gaactcaggc gatcccggcc ggccacagaa
     6421 cgtggcgtgt tgcagcggca tgtgcgatcc cagcacgctg ctctgtatct gagcgatcgc
     6481 cgaggaactg gatgtcgagc aagggggagg gactggcgtt cctcccccta gctttactcg
     6541 tgatcttgca ggaccggcta cgtattcgct aataccgtca gggcggcggc gcggcgggag
     6601 tcgacattga gcttcgacag gatcgactcg acgtgctttg agaccgtgcg gcgggagatg
     6661 tagaggcgtt cggagatttc cctgtctgtc agcccctcgc ggaccaagcg cagaacctcg
     6721 atttctcgcc gggttaagcc gagttccgct tcgccgtcgc tcactgaggt tcctgagagc
     6781 tcagccctta acctcctcgc atcttcgatt gccatttcga gggtaagcgc gctcccagcc
     6841 tcccactcca ggccgaacgc ctcctctccg agcgaacgcc gaacctcatc ccgcagctgc
     6901 gcgtaatagt ccccgatcga cgggcgctcc ggtgtgccga tcgcatcgag cacacgcgtc
     6961 gccgcgccca tcagacgggc cgcgcggcgc gcatccccct cggccgccgc aacgacgccc
     7021 agcccccgga tcgcccgggc ggtattccag gaatcaccgc cgctcagctc caacatttcg
     7081 aggaaggcat tccgggcgac tttccgtttg ccgagcagca gcgccgttcg tccgtacggc
     7141 tccaggctgg tcaggacata accgtgatcg ctcagccctc gaaactcagc aagcgcctcc
     7201 ccatggcaat cgaatgcggt ctcgatatcg cctgacacga atgcggtgat accgagcaga
     7261 tgagtcgtgt acgccacatc ccacgcactg ccgatctcgc gggatcgctt gcgagattcg
     7321 gtcaggagcc tggtcgccgt ttccggatcg cccgtgtcga tggcgacact cccgataccg
     7381 cggagggcaa ttgccagttg cgtgccgtct cccgaggcac ggcagacctc ctcggcccgg
     7441 atgtaccatg ccgtggccgc gtcatactca ttcagccagt gttcgatccc gcccgcgcac
     7501 aagagtccgg tggcgaagca acgaggcgaa ggatctgtgc ccggcatcga cagcacgcgc
     7561 tccagccttg ctcttccttc ctgcaatcgg ccgggactcc agagccagcc gatgccgccg
     7621 gccagccgca cggcagagcc cgcatctccc cgggcgtcgg cgtggtcaag cgccaaccgg
     7681 acattatcca gttcggcctc gccccacgct gtccatttcg ccagatccgg acccgtcact
     7741 tcgatcgaga accgctcggc gaagccggcg caccactccc cgtgggcagc ccgggtttct
     7801 tcgacctcac ctgcctggtc gagctgctcc atcgcgaact cgcgaatcgt ctcgagcatc
     7861 aggtagcgcg gcacatcggc caccgggacc gggcggatga ggctcttctc aaccagagag
     7921 cgtacgtgac cgagaacctc cgcccgatcg accgcatccg ccgcgacctc ctcgatcgcc
     7981 tccaggcaga aaccgcccac aaagacactg gctcgccgga acacagattg ttcgtccggt
     8041 gacagaaggt cgtaactcca tgcgatcgag tcgcgcattg tctgatgtcg ggtcgggcta
     8101 tcgcacctgc cgccggtcag cattggcaag cgcgcgttga gccgatcaag tagctgggtg
     8161 aggggcaata cgcccaccca agctgctgcc agctcgatcg cgagtggcaa cccatcgagc
     8221 cggcgacaaa tcgcggcgat aacgtcgaac cttttcgagt ccgggaccag ggacgtgttg
     8281 gtcgcgcgct cggcaaagag gcgaaccgcg tcatgctctc cgagggagcc gctgcccgcg
     8341 tctggaacgt cgagcggagg cacggcgaag gcatgctcgc ccgaaacctg cagatgtgcc
     8401 cggctcgtcg ccaggattgt caacattgga cacgccgtca gcagccggcc gaccagcggg
     8461 gccgcgccaa gcaggtgctc gaagttatcg aggatgagga gcgcgcgacc agcctgcagc
     8521 gcggccgtga tctgctcctc ggccgtctgc tgccctgcat tcggcggcgc gacgacctgc
     8581 gcaattgcgc cagcgacgag ctccgaatcc tgaatcgacg ctagggacac gaaccagacg
     8641 ccactcgggt acccactcgc tacacgctcc gccaggtgga tcgagaggcg agtcttgccg
     8701 acgccgcccg gcccggtgag tgtgaccagc cggatgcggg gatcagccag gagcgcgacc
     8761 acggcctcgg tctcgtgctc ccggccaatg aaggaagtca gcggccgggg cagcgcggtc
     8821 agatgctgcc ggccggtcga gggtcgtatt ggcgtcggct ccagcgtggt gcgggcttcg
     8881 ggggctgacg acatggcgga tcccgatatt caacatgttt ggtcgatcaa tcttggggaa
     8941 cgagccgcgt tctgtcaatt gaacggtcag cgcctcatcc ccccttcgcc gattgacggg
     9001 cgtcgcacgt tcggggatgc tgcgacgatg gaacagtcgc tcgctcattc gcatcgcgat
     9061 gtgctgcgcg gatatctggt tctgccgcat gccgtgccgg tcatcgtggt gctggcggcg
     9121 acggcggcgt ttgggctgat cgcggcaagt ggctggccgg ggctcggtcc gatgctgcgg
     9181 ttgctcgggg cgatgctcgg cgcgcagctt gcgatcggcg cggtcaacga gctggccgat
     9241 gccgacctcg atgcgatcgc caagccagat aagccgatcc cgggcgggtt agtctcgcgg
     9301 cgcggggcgg ggatcgtcgc cggctgcggg atcggcatga tggccgcgct cagcctgacc
     9361 ttcggggtcg cctcgttctt gctctgcacg atcgggacgc tggtcgggat cgcctacagt
     9421 ctctggttca aacgatcgat ctggtcgtgg gtgccctacc tgatcgcgtt gccgctgctc
     9481 ccgatctggg tctggaccgc gctcgacacg ctcccgaccg cactgctggc gatctacccg
     9541 atcggcgccg cggcaatcat tgccgtgcaa atcgcccagt ctttgccgga tgttacggtc
     9601 gatcaggaaa cgaatgtgcg gacgctggcc accgtgctgg gaccgaatct cgctcgcaac
     9661 gtctgctgga tcgcgctcct ggtcgcggtg ggactcgcgg cgatctgcgc cccatggttg
     9721 accgatcgtc cgggcttcgt ctggctggct ggtctgatcg cggtcgggct aatcggcgtc
     9781 aacgttgcga tctggtcgcg ggatgcgcgg cgcggcgttc tggccgcctt cccctgcgtc
     9841 gcatgcgccg cggtcatcct cggtttggga tgggctgccg ccctcgccgc tggttgacac
     9901 gtggcgcggc ccgcctctgg gccgcttcga tgtcacgcgg gtcggttgag gaaccagttc
     9961 tgctccatca ttggtacgcc ttgtcagcgc caagccgccc ggggctagcg ctaggactag
    10021 cgttatgtca tttcggtcag tctgcggcat cctcatcgtt ctcggccatc agccgcaaca
    10081 gagtcgtcat gcgattgatc cgcccttcgt cccagcgaac cctctcgaag aggatgcgca
    10141 tcgcctcgac cgcctcggtt cgatcggccg gcggttcggg cggcaggatc ttcgccacgg
    10201 ctgcaggctt cagcggacga cccgcgaggc gccgaaccag gttgggatcg gccatgcacg
    10261 ccgcggcaag gacatcgcac gaggccgcgc tcggacgctg atcgcctcgc acccatgcac
    10321 gtaagcgtct ccggtcaact cgcgcctcgc gtgccagatc ggccagcgac cattgccgcc
    10381 agatcaactg ttggttcagc cactcccaga atgtcccggg agccggacca tcccgcctga
    10441 ccgatccgaa cgtcgagcca cgcagcgcca gtacgcgctg cctgacgaga gcgagatcca
    10501 gcccgaagcc ggtgaggatc gtcacggcgg atccggtccc cgcctcgatg acgcccagga
    10561 gcaagtcggc agaatcgacc gggcacggtt catgtggcca ccacttgtat cgaacctggc
    10621 cggcgacctt cgctcgcgcg tgccggcgcc gcgccgaaat gagcgccagc tcaatagcat
    10681 tccaggctcg aggcgacaac ggaattggcc ggactatgtc gctctggggc tgattgctca
    10741 ccccgcgggc aaagcggacc gccagtgtga gccgcttctc ggaaagtccc aatccactca
    10801 gggcgcgcgc ggcttcgcaa tcgcgatccg caacgaatcc gagtaacagg tggtcagtcc
    10861 cgacagtttc atgacccagc gagtgtgcga tgtttcgggc aaaccgcagg attgtgcgag
    10921 cccggccgcc gacatcatcc tcgctcaggt tcgcatacgc ctgagcgcgc tcgagatcgt
    10981 cgtgcgctct tcgctcatcg cgaatgcgct ccagcaccgg gccgatcggc cgccgatcac
    11041 catcggacgc atacatcgag tagaggctcg accggagctc gtcgacatcg accgctagtt
    11101 gcttgaagat ccctgcggca gcggacgtcg gcatacccag tatgcccagg agcaggtgga
    11161 ccgtctggat ctggtcgtga ccaagacgcc ggcgttcctg gattgcggtt tcgagcgcta
    11221 atcgggcatg cgccgtcagg tcgagcgacc ggaggagggg cccgtcgctc ctgtccacca
    11281 tgcgctcgat cgccctcgtc acatccttga gccgcacgcc gagcaggccc agcagatagc
    11341 ccgcgagatc ctcgcgctcc tccacgacgc ccagcaagag gtgctccgtg ccgatactgg
    11401 gatgattcat tcgctcggca gcggcacggg caaactgaag cgcggtccgc gccgacggac
    11461 tgaatagttc gagttcggcc atcacgccac gtccgccgcc gtctccgccg cgagcgcaga
    11521 ggctcgggcc ggacccggcc atatgtcatt gaaacccgag gatgctggcg ggaatgtacg
    11581 cgttggtgac tttacctgtc aaagtacgtg ccaggggtca taattgcgcc gatcgcggca
    11641 ggtgcccggt cccctatccc gaggagtcgt ttgtgtctgg ttcggaaatc ctgctcgtcg
    11701 cgctcgcgat cctggttccg ctggcgatcg cgatcgcggt caccctctgg accctgcagc
    11761 cggcggtcct gcgcaatgag cgggccaagc gcgctcgcaa acgcttcgcc caggcacctc
    11821 cccggaaggc tgccgaagag cccggctcgc acccggagta acccctctct ccacaccggt
    11881 tctgggtcaa tttggctaac cagccgattg agccgctctc cctggccttc ctatgatgcg
    11941 agaacgtacg gaaacgcctg ccaacccggc cccggcgtta tgtgctgtcg aaccgcgtcg
    12001 gcgcgctcgg cagtctcttt gcgctacgtt ccgcaagggg gtctcagtgg agctgaagga
    12061 tcttattacc ggcgcccagg aggcgatgga ccagggcgat ttccgcctgg cgatcgctgc
    12121 cggcgagcac gcgctggtcg ggtacgatgc ctgcctttcc gcgcatcgca tgctcggcga
    12181 ggcttatctc gagcgtggcg atcagcagac ggcgatcggc cacttcgaac ggacgctggc
    12241 aatcgatccg ctcaatgtgg tcgcccgtct gggtctcggc gtcgcctccg aggagacccg
    12301 gcgctacgaa gatgcctacg gccactacct ctgcgcgtgg gagaccaacc cagcgctgga
    12361 tcaggttcgc gatgagctgg ttcgcctgcg cggagtgctc aacgtcgagg ggcggctcca
    12421 tccgacccgc gccggcctgg ccgggatcca cgcgcgctgc ggccaattcg gccgggcagc
    12481 gggagaatgg cgcgcggtgc tgtcgctcga gcccgatagc tcccgggccc gaaccgcact
    12541 ggccgagctg ttgtggcgac agggggacga tgccggcgcg tccgcggccg cgcgcgaggc
    12601 cttgcgcggt tgtccggaaa acgcccgcgc gctgctgatc ctggccgatg tcgaaaagcg
    12661 ccgcaacggg ccgaccgcgg ccgatctgat ccaacgctac agtcaggtgg acccagccgg
    12721 cgagatcgtc gagacgatgc gtcactggcg atccgatgtc gacctcgatg acctcctggc
    12781 cggcgcggcg ctgatcgata ccttcgattt cgagagcaac cgctccaggc agaccagcgg
    12841 gctgagccgg aagggcatca ccagcggcct ggcaaccagt cagatggccg cgccggacct
    12901 ctgggacacg ctcgtccagg atctggcgaa cggtgtcccg agcaagcccg caccgaccct
    12961 cgcgaccggt gttgagccat tcagctgggc cgatggcgca ccgccggccg agctggcatc
    13021 acctgcagcg gagccaccgg cgcagccggt cgtggcaacc aagcctgccg acagcgacat
    13081 cttcccggaa ctggcttcca tggacgcgtt tgttgtccct gatgtcgagg aaactgccgc
    13141 tcccgaagtt gatcaacccg tcgagcctga ggacgatctg ctttcgattt tcagccgtac
    13201 agacgctgct ataccggatg tcagtattcc atctgccgac gcagcagcgc cgtcggatga
    13261 cttcgacttc gatctcgtgc cgttctcgct ggacgaactc accggagtcg tcgcgcccag
    13321 ccatgaggtc gacgacgcgg cccttgccgc cacattcggc gtcgcagccg atcccctgcc
    13381 tgagcctccc gccgcaccgg ttgcgccggt cccgccggtg attccggagg agccggttgt
    13441 cgcggccgcc gcgcccgagc cctacgtcga cccgttcgtg tcggccgatg gccggatcga
    13501 tctgaccgcc ggatgggaca cgctcgatcg cgtgctggag gcggccactc cgggcgccag
    13561 tggcgagggc gggtttgacg ccctgatcgc cgaacttggc gtcgatgggg tcgtgccgtt
    13621 tgatggcgcg gtcgagagtg cggatgagga tgcgtggtcg cccttctcgg atgtcgactt
    13681 cgacgtcccg gtcgccgggg caccggtcag gaatgagccg ttcgacatcg tgatcgatcc
    13741 ctcggtgccg gacgccactg gcgagacgct ggacctgtcg gatgtcgagg cgctcgatat
    13801 caacaccttc gtcgcacccg agccagagcc cgttgcggag cagcggcagg agccggcgct
    13861 cggcagcgat atcctggccg gaattccgct cccggagcca agtggctata cgcagctcct
    13921 gcgcaatgtc gacgaggaga cgctgccgga tcacgcgggc gatgatgaga tcgatccctt
    13981 cgcgaacccg gatgccacgg gagagccgct ggcctttgat gagctgatcg gggtcacgag
    14041 ctccgacggg accggcccgc tcgcgccgat cgcggccgaa ccagccgagg tcgatctcgc
    14101 cgccgagctt gacttctcga ggaccgatct gtcgagtgcg gtgccgcagg tcgacgccga
    14161 gccgttcgac tttggcatcg atggcgtcga gccgttcgac accggcggat tcgatctggc
    14221 ggctacgatc gagtccgaat cggcatatgg ccaggtgcgt cccttctctg aaccggaacc
    14281 ggagcccgca gtcagtgacc tgccgctcgc tgccgaactg gccgacatcg tgccgttcgc
    14341 gttcgacgac ggcagcctga acggggtcga cggctcgccg gtcgacttct ctgagctgga
    14401 tgtcgcgcct gagccgatgc tcttcgcgcc gccggcggtc gctgagccgg agccgattct
    14461 cttcgaggag gcggagcccg cgcagttcga gtacgaagct ccggccgctg agctcgaagt
    14521 cgagtcgcca cccgccgaca cctggttcga tggcgaggag ccggtcgagg tggccgcgga
    14581 gcttgatgag gcatcggtcg atgacgaacc tgaagatctc ctcgtcgccg aagtggatat
    14641 ggcggagccg attgagccga tcctcgcacc ggtaggcatt cgatccgtga gctggccgca
    14701 gttcgtgggc cagacctcgg agttgattga ccggccggtg gagggcggca gcctcttcgc
    14761 tcgaatcgcc gcgcagaagg aggcgctggt cgagatgggt gtcgtggcta ccggcagacg
    14821 cctgataccg atcccgcagg tgacagagcc ggcggtggcg gtgctcgagc cggtcatcgc
    14881 gatcgccgag ccggtggtta agccgccgga gccgccagtt gcagccgctg ccgaagcaga
    14941 ggagcagatc cgcatcgatc tgatgggcat gcgggtccgg ctgattgagg acgaatcgtc
    15001 ggcgcgcgag atcgcggata cgttggaaga ggcaatcgcc ggaggtctgc aatcgccgct
    15061 ggcgttgcgg gtcctgggcg aggcatatct gaagttgggc atggtcgagc gggcggcggc
    15121 ccagttccgg caggcgatgt tgacccgccg ccggggcgga taaggagacg caggatgagc
    15181 gcggtactgg aacggacgga tcagcgaacc acgtcggtgg atgtgatcct gcgcacgctg
    15241 ctcgcctacg acgaagtcgt cggggcggtc gcggtcaata ctgagggcct ggtgatgggc
    15301 tcggccggga tgaccgagac cgacgtcgat gtcgtcagcc tgctgggtgc gtccctgacg
    15361 ggcgtggcgg agcggaccac ccgtcgactt ggaacgggat cggcggtcgg aatctcgctg
    15421 gtcacgaccg acggcatgat cacgatcggc aatggcggcg acttcgcagt catggtcttc
    15481 agcgcgcggt gcgactccgt ggcgctactc gaggcgctcg cggatccaat cagccggatt
    15541 cgtgcgatcg tcgatcccac ctgatctcag tcgattcaaa ctcggcaatt ggcatacggt
    15601 cggccctcgg gccggccgtt gtcgtatgct aggacccgtt ttgccgggcg cgacgcgcgt
    15661 atcggatggg tcctgacaga ggggaatgcc tggtggcaga tactgagcag acattgatca
    15721 tcgtcaagcc ggatggggtc cagcgcggcc ttgtcggcga gatcgttggc cggttggagc
    15781 ggcgcggcct gaagatcgtg gcgatgcaga tgcaggaagt ctcgcgggag acagccgagc
    15841 ggcactatgc cgagcacgag ggcaagccct tcttcgccgg gcttgtggat tacatcacgt
    15901 cggcgccggt ggtcgtgatg gtgctggagg gcccgcaggc ggtcgcagcg gcgcgatcga
    15961 cgatcggctc gacgaagccg gttgaggcga cgccgggcac aatccggggc gatctcggca
    16021 tgatggtggg ccggaatctg gtccatggct cggacagtcc ggagagcggc aagcgcgaga
    16081 tcgggatctt cttcggcgat tcggcgacct acggctacgc gcgcgacatg gaccgctgga
    16141 tcctcgaata agagcgtttc acgtgaaaca tgaaaagagc cgggtggcag acgccacccg
    16201 gctctttgtc aactgtcggc gctcagcctg ggttaatcgt ccgtgatgta gaggatcgtc
    16261 tgctgctggt tgaaccggga catcacctcg ccgatcagat tccgcaactc ctgatcgctg
    16321 acgtctcccg gtagttcccg ccggtcgacg accatctggc cggcgtactt ttcgcggctg
    16381 gccgaggagc gtgtgtcgta gcgcgtcgag cggtacgcaa tatcggcgta gaggcgctcg
    16441 ccgtcggtgg ttacgtagac gctgtgggcg gggtcgatct cgatcccgac cacgccgcgc
    16501 ttcacctcgt cgtgaatgac cgggtaaccg gcgggaaccc ggtatccata ttcctgctgc
    16561 gcaaatgtga gcatcggcaa aaaccgatcc cgaatcgcac tcaactgatc aaccggcacg
    16621 tctgctcctc gccgaagaca catcaacccc gcagattatc ccggacagcc cgaaccaatg
    16681 tcgagcatgt ttcacgtgaa actaccgctc gaccccgacc ggcgcctcgt ccgagagctg
    16741 gctcgcccgt ggcgtgagct gaccggcgta tttgttgttc cgcttcatcc ggtatggaac
    16801 cgagaccggt gagctgacct cctcgaacga gaaggcgcaa attcgcatcc ccggataaag
    16861 ggcaactgcc attcggccga gattgctcag ctccatagtc gccgtgccca cccagcccgg
    16921 ctcgaagacg gctgcggtcg aatggaccgt gattcccagc cgcgcgatac tggagcgacc
    16981 ctccagccgt ccaagcaggt tgtccggcaa ctcgagcgac tcgacggtcg aggccagcgc
    17041 gaaatcccca ggctgcatga tgaatggctc ctcgggcgca acctcgatcg tacgcatcgc
    17101 atcaccgatc gattgcggac tgcgcggatc gatgaagctg tggcggctgt gctcgaagac
    17161 catgaaggtg gtcccgagcc ggaaatcgac cgagaccgag ccgagttgcg catcgagatc
    17221 tggctcaggc gtgatcctga ttcgaccgtc ggccagggcc ttccgtatat ccacatccga
    17281 caagatcatc aggaaacgcc ccttcgcccg cacgcgttca acgaatcaac gacaccaatt
    17341 gtgccagcgt tccgatccgc atgccgcctg agactcccag gcacactcgc cctcggaacg
    17401 cgtcgacaca gatggtagac tctcgtccga catgtcttcc agacacgcct cctgcctcat
    17461 ggactgttga gtggctgaca gcgcgacccc gtcctatttc caacgtgtcc accggcgcgc
    17521 gcagcaccgc gcgaccacgg tcgaagctgc ccgcatctct gcgtcgcgcc aaccgaacgt
    17581 cggccagcgg cgctataagc aaatcaagca atccgccccg caggcggaca caccggcgcc
    17641 tcccacccca ccgcgccagt cgacactcgt caaaacgaca ccgattgcgt cactcggcag
    17701 cgagctggcc cagctcaagg cgcggcagga aaagcaccac gggccgaagt ggtggtccgg
    17761 catcgcgtcg cacccggtcc gatcggcctt cctggccgtc atcatcctct ccctctccgg
    17821 cgttctggct atcgccggtc cgatcgccta ccgctcctac cgggcctacc aggatgtctt
    17881 cgtgacccag gtgccagacc aggagagtgc cttccacgcc gagatcaatc ccgaaggcac
    17941 gcctgtcctc gtcgccaacc aggcccagga gtcggcctcc atccccgaat gggatggcac
    18001 cagccgcctg acgctcctcc tgctcggcgt ggacaagcgc gagaacgact atgcccgctc
    18061 cgacacgatc atcctggtca atatcgatcc ggtcgcgaag acggcacgga tgctgtcgat
    18121 cccccgcgac ctgaaagtca tcatccccgg cttcggcgcg cacaagatca acgctgccta
    18181 tgccttcggc gacgccaaca agaacgtgcc gggcggcggc ccgggactga tgatgcggac
    18241 gatcgagacc aacttcggca tcaatatcga ccacttcgcc gaggtcgact tcaacggctt
    18301 tgtcaagatc gtcgataccg tcggcggcgt gacgctcgac gtcccgtacc cgatccgcga
    18361 caacgagtat ccggcgccaa acaaccagta catgcgcatc ttcttcccat ccggttggca
    18421 gcatatggac ggccagcgcg tgctccaata cgcccgaacc cgccacgacg acggcgacgg
    18481 ccgccggtcc gcccggcagc agcaggtgct gctcgccctg cggcagcagg cactcgatct
    18541 caacctgctc aagcaggcgc cgactctgat ctccgaagtg ggggatacgg tgcgcaccga
    18601 tctcagctcc ggccaggcgc tacagctggc gcgtctggcg acggagatca atcagtcgga
    18661 gatccagcaa ctcaccctcg acccggcctt gattacgtgg cagaccgacg agttctattt
    18721 ccagggcgac tgggcgatga tcggcgatat catgaccgaa ttcacgggcc agaagatcgt
    18781 cccgccgatg tccgcgctgg ccaatccaag ctacgaaacg acaatctcga tcctcgacgg
    18841 gacgttgatc ccgggactcg gaccgcgcgt gctcgatgtc gtcaagagca acggcttcgc
    18901 ggattcgacg accgtggacc taccggacgc cggaaattac ccggagacga agatcatcgt
    18961 ctcgccggac aacatcgcca ccgcctacct gctcgccggt atcctcggcg tgccggagac
    19021 cgcgatcacc gcaagcgacg atatccagac cccgaccatc gtcgccacga acaccgccac
    19081 cgcgacctct gccgcggcgc cggtcggcac cccgaccgcc gggacgccga ctgccgatgg
    19141 caagctcgat gtagtcccgc tgttcccgac ggccgtcccc gagtccggca aggccagccc
    19201 agcgcccgcc ggggagatca tcatcatcct cggcgacgat ctgccggatc ccgcctggta
    19261 caacgtcgac cccaacgcgc agtaacccct catccgttgt ggcggggctc catacccgac
    19321 tctaatttca aggaaagcct ggtcggacgt gcaacgtaga cgaagcgcgg ggttaccacg
    19381 ccccgcgcta catctggtgg gcggtgcata tgaaggtggc gaccgggagc ccagtcgggt
    19441 acggagccgc cactacgagg ctttcgttgc caggaagagg aggtcgccgg cgaagatgta
    19501 cctcatccag cgcatcgcaa aggtatcgtt cggcacggtg aggcggagga ggccggtccc
    19561 ctcgacgaca tcgacctcgt agccctcgag atcctccgtg tagggccgct tcgagatcgg
    19621 agaggtcagg gtatcgatgc ggtcctgaac caccgtcacg gcggatttgg catcggccac
    19681 gtcgcccagg tcggcgatca gagacacaag ctggccatcc ttcttcgctt cgatgccgcc
    19741 caggaagagg tgcgccggcg gaatcgtctc gccgtccacc aagccgcccg gattgaccgt
    19801 cggcgaaccc agcagcgcac cgttcagcag cacatagccg tgcatccctt cgatcacttt
    19861 cgccacatcc gcgaacagct cgccgtccgc gatcgactgg gcctcattcg ctgccacgtc
    19921 gattgccgcc gccaggacct cggcggatcg ggaagcgatc acgagcgact ccgtcacacc
    19981 gacatggttg aatcgcccga gtgcataccg gtccagatcg cgggaagggt ccaagccatc
    20041 ttcgggattg accagtacgc ggaagtctcc gttcgtgctc gcggagtagc tccactgcgc
    20101 aaatgcctgc tcgacgcgct ccgggtcgaa cgcgcccgaa tagatgcgga gctgatccgg
    20161 tggatcgaat gcgaccgcga gttggtcgat gtcccacggc tcgaagccga cgatctcgcc
    20221 ccatggcgca ttcatgaaca ggagcaacgt gtcgtcgttc atcgggatcc gctggccaat
    20281 ctggctccag acctttaccg tctcctcgcc gaaggaattc ggcttctcga ttccggccgc
    20341 ctcgcagtag ccggctatat ccgcccaggc gacgatcgag gtcggccatg cgccctgatc
    20401 atccggcagg gcggggaaat gggcgagcgc caagtcatcc gcgatcttgc ccagcttctg
    20461 ggcgctcact ccccgcgaga tcagtgccgc ggccaaggcg gaggcaagga cagcacgacg
    20521 cgacgttttc actgacacag ttaaagactc ctcaagtgga aaccagacaa cgaactctgt
    20581 ttgtgcggtg gattagtaga gctgcatccg gagcgtcatc gcgccggagg cggccttgta
    20641 cgcggcgccg acgctcgtgc cggtatgaat gcgctggatc gtctcgccca gcaaaggagc
    20701 gaccgacagc atcgtcagct tgtcgatccg cttatgctgc ggcacctgaa tcgtgtccgt
    20761 aaccacgatt tcgcgaatca cactcttctg cagccgctca actgccggcc cggagaggac
    20821 cggatgcacg caggtcgcgt agacctccgt cgcgccttcc tccatgcaga cctgcgcggc
    20881 ctgcgtgatc gaaccggccg tatcgatctc gtcgtcgacg atcacgacct ggcagccggc
    20941 caccgagccg atcaggttca gtacctgcga gcgatcctgg ttgccgattc gccgcttttc
    21001 gatgatcgcg agcggagcgt cgaggatttg ggcgaagttc cgggcccgct tggtgttgcc
    21061 gaggtccggc gagacaacga caggattctc cagccgcttg tcgacgaagt agttggccgt
    21121 caggaacatc gcgctcatct catcgaccgg gatgtcgaag aagccctgga tctgcccggc
    21181 atggagatcg atcgccagca cgcggtccgc acctgagacg ccgaccagct tggcaaccag
    21241 gcaggccgtg atcggcaccc gcggctggtc cttcttgtcc gtgcggccgt agccgaagta
    21301 ggggatcacg gcggtgatcc gaccagcgga agcgcggcgg agcgtatcga tcatgatcag
    21361 cagctccatg atcgaatcgt tgaccggctc ggcgaaggac tggattacga aaacatcgtt
    21421 ttcccgcacg ctttcaccga tccgcacgaa ggtattgtcg ttgctgaact tgaagacctc
    21481 cgcgcgaccc cgcggtaagc caaggtaggc gcagacctgg tcggtcaacg ccgggttgga
    21541 gttgccgccg aagatggtca attcgtcgta caccctagcc atcgagttct gttccacctt
    21601 tcggagcgct gggcttccgt cgtttgcgca ccgatcgggt tgcgcgtcgt ttgtgcagct
    21661 cgccgacccg ttccagagcc tcagtcgcgg cggcctgctc ggccagtcgc ttgctctcgc
    21721 cggtcccgga gccgagcacg gaatcgccga tcagcacctc agccgtgaag gtgcgggcgt
    21781 gatccgggcc ctcctcgcca ataatgcggt agatcggcgg ctgccggtag tggtcctgca
    21841 cgtattcctg gagttggccc ttcgggttgg ccgcgatctg atcctcgagc gcggcgaagg
    21901 catcggcgtc gagaaacggc tccatgaacg cccgggctgc ttcaataccc tgatcaagcg
    21961 tgatcgcgcc caccactgcc tcgaacgccc cggagagaat ccggtcgcga tcccggtcgg
    22021 aaacccgttc tccggttgcc agatagacat aatttccgag tttgagtgac cgcgcccagg
    22081 ctaccagccg ctcgctccgg accagcgcca cccgccgggc ggtcagatcc ccttcgggcg
    22141 cctccgggaa tcgccggtag agatcggccg cgacgatgta tccgatcaca gcgtccccga
    22201 cgaactcgag ccgctcgttt gatcgctggg acggcggcaa ctccagctcc ggcgagattg
    22261 agcgcagttc gtgggccacg gagcgatgag tcagcgccaa ccgcagcaca tcaagatcgt
    22321 ggaaggcgag cccgagcgac gcggcaaagc gggtggcgtt gtcttccgct tcgccagcag
    22381 tcgattcgat ggtctggggc ttggcgcggc gactcatgaa cattcccggg gtaaggcccg
    22441 gcaacgagcg ccgggtccgt gccgattata cggactgcgc cgcccgctca accgccggtc
    22501 agcaccgtcg attgcccacg ccgcggcaac ggggtaggct atccgcaacg gccgacgtgg
    22561 ccgttgtcgg acgggagccc gggaacgggc tgagagggcg agccgtctcg ccgaccgttg
    22621 cacctgatca gggtcatgcc tgcgaaggga ccgcaccgcg cgacaagcgc ttgtccccca
    22681 atccgacaga ccaccatcgc gagtgaggag cgcctgcaat gccgctgacc cgtcgttcgt
    22741 tcctggccgg tagcctcggt gcggccgctg ccttcggcac tgccagtccc ggccgcgcgc
    22801 agggaccggc aaaggcttcg atcgcgctcg actggtaccc caacgccaac cacgccggtc
    22861 tgttctgggc gcaggcgaag aacctcttct cggacgccgg catcgacata acgatccaaa
    22921 caccggccga tccgaccacg gtgcttcaga cggtcgccgc cggtcgcgac accttcggaa
    22981 tctcctacca gcccgatatt ctgctcgccc gcgcgcagga tgtgccggtc gtcgccgtct
    23041 gcgcgatcgt accgcgaccg ctgcagggcg tgatgtcgct caagagcgcg ggcatcgcac
    23101 gtccggccga tctgcgcggc aagaccgtcg gataccccgg tattccgagc caggaagcgt
    23161 tcctcaagac catgctggcc gccgacggtg tggcaatgga tcagatcacg ctcaacaacg
    23221 tcgagttcaa cttgctgccg accctgatct ccgggcaagc agcggcagta atgggcgcat
    23281 tctggaccca cgagacgatc gtcgccgaac tcgagggcta cccggtcgag atgatgaagg
    23341 tcgaggactg gggcgtgccg atctacgacg agctggtgct ggtcacgagc gaggagacgc
    23401 tggccaacaa gcgggagttg gtgaccggcg tgctcgaggt cgtccgcgcc ggatatctgg
    23461 ccgcagcggc cgaccagaag caggcagtcg acatcctcgt cgccgcgtat ccggagactg
    23521 agcgaccggt cgaggagcag ggcatcgcgc tgctggccga cctctggacc cagcccgttc
    23581 ccggcttcgg catcatggac ccggatgtct gggcgaagtt cgccgattgg atgatcgagc
    23641 acgagctgct gccggccggc ttcgaactga ataacaccat cggcgccaat ctgcccgcgc
    23701 aaccgttagc gactccgacc aggtaactca ctccgcggac actccgcggg cgcccaaagc
    23761 cgaaccggcc gacttgggcg gcaactcgcc gctcacgcca gaagggacga gtacgattgt
    23821 gcctgtccct ttttcctgca caggtaatat ttgctggttc cgcccatcgg tacttccgat
    23881 ggatactcgc gccgcgctcg ccgccatagc ctctagacac gcgcacgcga gtgtccggcc
    23941 cggcgcgaga ctggagcgga ccacgattcc ccggaggctg ttaccaccgt gtctgacgat
    24001 tcgccctttc cgtcccgaag ctggctgccc actttgaatg ccggggaccg cttcactacg
    24061 gcgaccgaaa cgaagaccgc caccgtcgaa ccggtcgccc ctgtggacga ggctgcaccg
    24121 gagccggccg agcgcaaccg caaccacctg ctcgctcagg attggtcgag cgcgctcgaa
    24181 tcccagttcg ggctgaccgc gccggtcgtc aaggtagagc cagaggagtc gatcgcggca
    24241 ctcaaggagc ggctcgcata ctacgagcac ttcgacgatc tgatccgcga caacattgcg
    24301 cgctcgtcgg cgttattcga agccgtattt gccgagcggg acaaggcgcg caaggtccag
    24361 tccgatgccg aatcgaccat gtcggcggtc gcggcggagg ccgatcggcg agttgcggcc
    24421 gagcgccagc acgttcagca catcctgatg tcgctgatgg acgaggcgac cttcctgcac
    24481 cagcgctccg acgccctgat ccagcgcctg gctgaggccc tgaccgagat ggcccctacc
    24541 agcaacgagg ccgacgaacc cgtctccggc gcctgaacgc caactccaca tgaacatcaa
    24601 acagtgcgcg aggccaatat gcctcgcgca ctgttcgtag tggcggggct ccgtacccgc
    24661 ctggagtccc cgaccgcatc actcaattgt atcgaacgtg catctgcctg gtcttgtcgg
    24721 acacgacgct catatgtatt gccccgatgt cagcggactg gctccatgga ccggattttc
    24781 gggcgagccg aggcgggtac ggagccccgc cactacgaat ccgagcccta tttcacgttg
    24841 atcgtgcgcg agggccagcc ggtggcgccg tcaggaagcg gggcttgctc gtcctccttc
    24901 atcacctcgc cctgaccatc ggtgacgcga atcttcatct tgagctggcc tgattgcgcc
    24961 gtgaacggga aggcccagcg cacccacgtg aagggcatat tgagcgccgg ctcgagctgc
    25021 gcatcggccc aggtcgcgcc gtcgtcgatg ctgacctcga cccgctgcac gccacgatcg
    25081 cctgcggacg ccatgccaca ggcagtgacc ggtccgg
//
LOCUS       DATAOZ010000641         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3404549, whole genome shotgun sequence.
ACCESSION   DATAOZ010000641 DATAOZ010000000
VERSION     DATAOZ010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3404549"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VHR64_16080"
     CDS             <1..340
                     /locus_tag="VHR64_16080"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX3304405.1"
                     /translation="ARHLLRGLGTSLLMSVVLAAAALLIHAATLTLNNANSAPSEPAG
                     GTNITARPSASAQAPRLYVIRPGDTLRTLAQRFYGDQALWPVLFEANRRRLQQAEDIQ
                     VGTQIIVPDR"
     gene            446..>1590
                     /locus_tag="VHR64_16085"
     CDS             446..>1590
                     /locus_tag="VHR64_16085"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX3304406.1"
                     /translation="MHRPRHCMIVNNTYPDVRVEREARALVDHGYAVDVMCMQGGNRP
                     IREMTGDIRVFRLPVWRRRGQGIRVQLTEYVSFLAWAAAMTALQHRRHHYTSVQVHNV
                     PDFLVFAAAFPKLAGIPLILDLHDLMPEFFASRFGREMDAPAVRAVLWEERVSTAFAD
                     KVLTVTDLWRERLVARGLSPDKVEVVMNLPDPALFPVQQPSVKPVGVPLTFLYHGSFT
                     YRYGIDVLLRAFAIASKDHHIRMIVHGRGEYQEDIRCLVSELDLGDRVELSTKWIPSD
                     LLPDLIRSADVGVVPYRRDLFTDGILPTKLMEYAALGVPAIASRTSAVGAYFTDEMVR
                     YVEPEDVSGLARAIAALAADPNERLAIATNAQRFTREHQWHDEALKYV"
BASE COUNT          286 a          511 c          501 g          292 t
ORIGIN      
        1 agcacggcat ctcctgcggg ggttgggaac cagcctcttg atgagcgtcg tactcgcggc
       61 ggcagccttg ctgatccatg ccgccaccct gaccctcaac aacgcgaatt ccgcgccgag
      121 cgagcctgca ggcggtacga atatcaccgc gcggccttcg gcgagtgccc aggcgccgcg
      181 gctgtatgtc atccgacccg gcgatacgct gcgcaccctg gcccaacgat tctatgggga
      241 ccaggccctg tggccggtgc tgttcgaggc aaaccgaagg cgcctacagc aggcggagga
      301 cattcaggtc ggtacacaga tcatcgtccc cgaccgctag cgacacgagc cgccatgacg
      361 gtggcacgac agttgcggat aatcgatggc cacctggcct tccgttccca acttcccacc
      421 tccgcgaccg cccgcaggag ccgcgatgca ccgtccgcgc cactgcatga tcgtcaacaa
      481 cacctacccg gacgttcgcg tggagcgcga ggcccgagcg ctcgttgacc acggctatgc
      541 ggtggatgtc atgtgcatgc agggcgggaa tcggccgatc cgggagatga cgggagacat
      601 ccgcgtcttc aggctgccag tctggcgacg ccgagggcag gggatccgcg tgcagctcac
      661 ggaatacgtc agcttcctgg cgtgggcagc ggcgatgacg gccttgcagc atcgccgcca
      721 ccattacacg tcggtccagg tgcataacgt gccggacttt ctcgtttttg ctgccgcatt
      781 cccgaagctc gctgggattc cgctgatcct cgatctgcac gatctgatgc cagagttctt
      841 cgccagccgg tttggtcgcg agatggatgc tccggcagta agagctgtcc tctgggagga
      901 gcgagtcagc acggcgttcg cagacaaggt cctgacagtg accgacctgt ggcgagaacg
      961 actggtcgcg cgcgggcttt cgccggacaa ggtcgaggtg gtcatgaacc tgcccgatcc
     1021 ggccctcttc ccggtgcagc agccgtcagt caagcctgtt ggcgtgccgt tgacatttct
     1081 ctatcacggc agcttcacgt atcgctacgg tatcgacgtc cttctcaggg ccttcgccat
     1141 cgcctcgaag gatcaccaca tccggatgat cgttcacggc agaggcgagt accaggagga
     1201 catccggtgc ctggtatccg aactggacct tggcgatcgc gtggagctga gcacgaagtg
     1261 gattccgtcg gatctgcttc ctgacctgat ccgcagcgcc gacgtggggg ttgttcccta
     1321 tcgtcgggac ctgttcaccg acgggattct gccaaccaag ctcatggaat atgcggcgct
     1381 cggggtgccg gcgattgcgt caaggacgag cgccgttggc gcctacttca ccgacgagat
     1441 ggtccgctac gtcgagccgg aagatgtcag cgggctggct cgcgcgatcg cggcattggc
     1501 ggcggacccc aacgaacggc tggccatcgc gacgaacgcg caacgcttca cccgtgaaca
     1561 ccagtggcat gacgaagccc tcaagtatgt
//
LOCUS       DATAOZ010000642         8332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_3404898, whole genome shotgun sequence.
ACCESSION   DATAOZ010000642 DATAOZ010000000
VERSION     DATAOZ010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3404898"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VHR64_16090"
     CDS             complement(<1..678)
                     /locus_tag="VHR64_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX3304407.1"
                     /translation="MGELVAGRPALKLQYQMSAPLDLVSGMALLYRAVPGSGLDPWLI
                     KARRALAPETQRDLDLLHGFSGRMLLYMEEPIWAFQPLLPERRDATIDELLAFLRELP
                     ADAYLDMVERALSRVNRELKNHAHAPARGDVDGWRAYLEPALTTALADDVLPLLANAE
                     ELKERTIGLIDGVWTQVYGPEYEAHVPELQEAVNRASRTGSAGFAPTFVELTENRLPS
                     ALVAGVHD"
     gene            947..2851
                     /gene="dxs"
                     /locus_tag="VHR64_16095"
     CDS             947..2851
                     /gene="dxs"
                     /locus_tag="VHR64_16095"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX3304408.1"
                     /translation="MTILSQISGPDDLKGRSFADLTQLAAEIRSELINTVTRTGGHLG
                     ANLGTVELSIALHTVFDSPRDLLVWDVGHQAYVHKLLTGRTDRFDTIRQEGGISGFLS
                     RDESPHDSFGAGHASTSISAALGMAVAQQYKPTAKRNHVVAVIGDGALTGGMAFEGLN
                     NAGSLQVPLIVVLNDNEMSIAPNVGALSKYLDRVRTEPKYKRAKSEISAMMERLPQGD
                     FLVELSKRWKDSFKEFVYHSMIWEELGFTYVGPVDGHDIRATVEALRSACRVNGPVFV
                     HVVTQKGRGYDPADQDRERAHAVSAVAAPTVTGAPPPPPKYQDVFAETLIELGRTNEK
                     IIGITAAMPSGTSLGKFQQAFPERFFDVGIAEQHAVTFAAGLATQGMKPVCAIYSTFL
                     QRAYDQIIHDVCIQRLPVIFAMDRAGFAGDDGRTHHGVFDISYLRCLPNMVIMAPKNE
                     NELRHMIKTAVDYQAGPIAVRYPRGAGVACDISEPLHSLPIGRSELLREGGRVALVGI
                     GSMVYPALGAADLLMNDGIEATVVNARFVKPLDETLLLDLADTHEAVITIEENVIDGG
                     FGSAVLELYAAHDKRIPVHTLGVPDRFFEQASQGRLREMAGLSPQQIAAAATELLTTG
                     AKPTPAVVTA"
     gene            complement(2940..3170)
                     /locus_tag="VHR64_16100"
     CDS             complement(2940..3170)
                     /locus_tag="VHR64_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304409.1"
                     /translation="MAARTNAHEERLATLRSVMNVIAILDQSASRRAAQNNGFDLRAL
                     IPSNWATDADESWDDFRLPAPVSQLGTSNLQS"
     gene            3385..4119
                     /gene="rsmG"
                     /locus_tag="VHR64_16105"
     CDS             3385..4119
                     /gene="rsmG"
                     /locus_tag="VHR64_16105"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX3304410.1"
                     /translation="MTELAIRPELLRDLPEDKQRQLRDYRDLLFRWNQRFNLTAIREP
                     EAIDGRLIADALRLLPTIDEELARQDGKLRLIDLGTGAGLPGLVLKIARPEIAIVLAD
                     ATNKKIQFVQYVVTELGLTSARTVHGRAEDIGHHLDYRGQFDLVTARGVASLPTLLEL
                     TVPLLVVGGAFIFPKGQDLDDELEMGQRAAKALGARIESADLLPGDTSEPVTRLVRGV
                     KIASTPGRYPRRAGIPNKEPLGRDGR"
     gene            4116..4682
                     /locus_tag="VHR64_16110"
     CDS             4116..4682
                     /locus_tag="VHR64_16110"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEX3304411.1"
                     /translation="MTKSRIFYRKEKLRGACWEYQIDGGWLTVLGDEERHILHHRFSF
                     YRPREVEVLRADGPVTLVRWGAEQWFFPDRWFSLLRFVTESDDTVGYYVNFSRPLTEL
                     KANYYQDLDLELDLWLHPNGVATELDRDEFNQEIECARLRPDWAAQVIEALGQVSSAV
                     SSSLAEFGPNLDAHRDPANGLPIFILRA"
     gene            complement(4679..5230)
                     /locus_tag="VHR64_16115"
     CDS             complement(4679..5230)
                     /locus_tag="VHR64_16115"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX3304412.1"
                     /translation="MFLGSREDIAHLGYVQGVCPKCGKTGSFTVYLAKRRMTISMFAA
                     LPMGEQHVLECRHCQARFAIPPEMKDQLQARLITADRLADLVEQLPDQSEREARPVRT
                     LYQTLQVDQDADPDVIEAAFKRLAMKYHPDRSKDPEAAAKMREVLQAKDVLADPKRRL
                     AYDRSIGIKREPPKPVALRPEDV"
     gene            complement(5251..6675)
                     /locus_tag="VHR64_16120"
     CDS             complement(5251..6675)
                     /locus_tag="VHR64_16120"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX3304413.1"
                     /translation="MSEHQSPAATRTERDSMGEMTVPADALYGATTARAVENFPISGI
                     AFSRSFIRALGLIKQAAAEANRDLGLLPEDRASLIVQAAQEVVDGRWDAEFPIDIFQT
                     GSGTSTNMNANEVIATRAVQIAGGGRPVHPNDDVNMCQSSNDVIPATIHVAAFLLIRE
                     SLIPALDHLELVLRRRAEETHDVIKTGRTHLMDAMPIRLGQQIGGWATQVAQARERIE
                     STLPRLAELALGGTAVGTGINAHPEYGAKVAAALAQRTGLPFVEGRDHFALQATQDTA
                     AELSGQLKAYAVGLMKIANDLRHMNSGPQAGLGEIVLPVLQPGSSIMPGKINPVISEA
                     VMMVCAQVIGNDLTVTIGAQMGNFELNVMLPVIAHNLLQSIELLASASNVLADKAVAG
                     FTINRQHIADLVDKNPIMVTALNSIIGYDLGAKIAKRAYAENRRVKDVAAEMTDLSPD
                     ELDRLLDPADLADGGIKGSAGGGG"
     gene            7016..7312
                     /locus_tag="VHR64_16125"
     CDS             7016..7312
                     /locus_tag="VHR64_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304414.1"
                     /translation="MQNPACYSCRFFQAGSTNAFGSCNHPNHQQSGVPITIRAQEVNC
                     YRGFGTSDWMPALIGDSAACEDIVLSERPAPYRAPWHGLTPIEQTETPAYGLRD"
     gene            complement(7313..>8332)
                     /locus_tag="VHR64_16130"
     CDS             complement(7313..>8332)
                     /locus_tag="VHR64_16130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3304415.1"
                     /translation="SLHFTVMAVCGMLASSTVAPAAARFGRRATCLASVAVVSFGLLA
                     LCLAPSVALTLPAAAVIGLAGPFAIILSQTEVLDRHPAHQATAMAELNLVVSVALMAT
                     TLGVGPIVALTDSWQIALLIPVLAAISAFIPLRGLEFSDASRPRTKGAARKMTGLAWL
                     FCLLIVCQAGFEWSYGYQAAEFLNKAGGFSKGTAATLMTVFYAGMVVCRVGLMWAVRR
                     YAASRLLLASFAMALAGFLLLAGGPTAVFKVVGLLLSGFGIAITFPMIITLAARAFPD
                     ATDWIVAKLYMAGGVAIAAAPFAIGALGDEIGIARSFWVLGILAALGLIVSPFLRRAL
                     ADTGG"
BASE COUNT         1512 a         2775 c         2545 g         1500 t
ORIGIN      
        1 atcgtgcaca ccggcgacca gcgccgaagg caaccggttc tcggtcagtt cgacgaaggt
       61 cggcgcgaac ccggccgaac cggtgcggga tgcgcggttg accgcctcct gcagctcagg
      121 cacgtgcgcc tcgtactccg gcccgtagac ctgggtccag acgccgtcga tcaggccgat
      181 cgtccgctcc ttcaactcct cggcatttgc caggagcggg agcacatcgt ccgccagggc
      241 cgtcgtcaat gccggctcaa gatacgcgcg ccagccgtcg acatcgccac gagccggcgc
      301 gtgcgcatga ttcttcaatt cccgattgac ccggctcagc gcccgctcga ccatgtcgag
      361 gtaggcatcg gccggcagct cccgcagaaa ggcaagcagc tcatcgatcg ttgcatcgcg
      421 gcgctccggc agcaacggct ggaaggccca gatcggctcc tccatataaa ggagcatccg
      481 gccggagaat ccgtgcagca gatcgagatc ccgctgcgtc tccggggcca gcgcccgccg
      541 cgccttgatc agccacgggt cgagtccgct gccagggact gcccggtaaa gcaacgccat
      601 gcccgacacc agatccagcg gtgcggacat ttgatattgc agtttcaggg ccggtcggcc
      661 ggcgactaac tcacccatga cattccacga atcttggcta cgcgatgatg gtatgactat
      721 atcaacatat gcaatcggat gcaagggtct gtcggcgact ttgtgtgcga attcacaaac
      781 cacccgccat ggaccggcgt agtactctgg tgcagtccgc ggttgactgc cgttgcatgc
      841 cgtgtggtag tgtccacggg acgcggacct cacctgctaa cgctctctat gattcgcgcg
      901 cgtatttcta tctcactcag ccgccggccc caggagtact tcggcaatga ccatcctctc
      961 gcagatttcg ggtccggacg accttaaagg tcgctcgttc gcggacctga cccagctcgc
     1021 tgccgagatc cgctccgaat tgatcaacac cgtaacgcgt accgggggtc atctcggcgc
     1081 caatctcggc acggtcgagc tcagcatcgc actccacacg gtgttcgact cgccgaggga
     1141 cttgctcgtc tgggatgtcg gccaccaggc ctacgttcac aagctgctga ccggccgcac
     1201 cgatcgcttt gacacgatcc ggcaggaggg cgggatttcc ggcttcctct cgcgggacga
     1261 gagtccgcac gactcattcg gcgccggaca tgcctcgacg tcgatctcag cggctctggg
     1321 catggcggtc gcgcagcagt acaagccgac ggccaagcgc aaccatgtcg tcgcggtcat
     1381 cggcgatggc gcgctgaccg gtgggatggc cttcgagggt ctcaacaacg ccggcagcct
     1441 tcaggtcccg ctgatcgtcg ttctcaatga caacgagatg tcgatcgccc cgaatgtcgg
     1501 cgcgctcagc aagtacctgg accgcgtccg gactgagccg aagtacaagc gagcgaagtc
     1561 cgagatcagc gcgatgatgg agcgtttgcc ccaaggcgat ttcctggtcg agctcagcaa
     1621 acgctggaag gattccttca aggagttcgt ctaccactcg atgatctggg aagagcttgg
     1681 cttcacctat gtgggaccag tcgatggtca cgatatccgc gccactgtcg aggcgttgcg
     1741 cagcgcctgc agggtcaatg gcccggtctt cgtccacgtc gtgacccaga agggccgcgg
     1801 ctacgacccg gccgaccagg accgggagcg ggcccatgcg gtctcggccg tagccgcgcc
     1861 gacagtgaca ggcgcgccgc ctccgccgcc gaaataccag gatgtcttcg cggagaccct
     1921 gatcgagctc ggccgcacga atgagaagat catcggcatt acggcggcga tgccatccgg
     1981 cacctcactc ggcaagttcc agcaggcctt tccggagcgc ttcttcgacg tcggtatcgc
     2041 cgagcagcac gccgtgacat tcgcggccgg tctggccacg cagggcatga agccagtctg
     2101 cgcgatctac tcaaccttcc tgcagcgcgc ctacgatcag atcatccacg atgtctgcat
     2161 ccagcgcctg ccggtcattt tcgccatgga tcgcgccgga tttgccggag acgacgggcg
     2221 aacccaccac ggcgtgttcg acatctcgta tctgcgctgc ctgccgaata tggtcatcat
     2281 ggcgccgaaa aacgagaacg agctgcgcca catgatcaag acggccgtcg actaccaggc
     2341 cgggccgatc gccgtccgct atccgcgggg cgctggggtc gcctgcgaca tctcggagcc
     2401 gctccactcc ctgccgatcg gccgctcgga gctcttgcgg gagggtggtc gtgtcgccct
     2461 ggtcgggatc ggctcgatgg tctacccggc gcttggggca gccgacctcc tgatgaacga
     2521 cgggatcgag gcaacggtgg tcaacgcccg cttcgtgaag ccgctcgacg agacattgct
     2581 gctcgacctc gctgacacgc acgaagcggt gatcacgatc gaagagaacg tgatcgacgg
     2641 tggattcggc tcagcagtgc ttgagctcta tgccgcccat gacaagcgca tcccggtgca
     2701 caccctcggg gtgccggacc ggttcttcga gcaggcctcg cagggacgcc ttcgcgagat
     2761 ggccggtctc tcgccacagc agatcgcggc agccgcgact gagttgctga cgaccggtgc
     2821 gaagccaaca cctgccgtcg tcacggcctg acggaatacg aatacgggcg gcaggattaa
     2881 cctgccgccg gtttcagttt tggcacgtag tttgcccgtc aacgctcggg cgtcggctgc
     2941 taggactgga gattgctggt gcccaactgg ctaacaggtg ccggcaggcg gaaatcatcc
     3001 cagctctcat cggcatcggt tgcccagttg gacgggatca gcgcgcgcag gtcgaagccg
     3061 ttgttctgcg ctgctcggcg cgaggctgat tgatcgagga tcgcgatcac gttcatgacc
     3121 gaccgaagtg tcgccagtcg ctcttcgtgc gcattggttc gtgcagccat tcccgccgtc
     3181 ctttcccgga taaagcattg cgccgcgggt gtttgcattc tgtcaactcc ggcgagtttg
     3241 gccactgtcc gtagtggtac tttcgttatg ttcaccgcag gtgttttcgt ttgcgggcgg
     3301 aaacgtccgc gcccactctc ccgttgaaga tcacacgtgc ctcagatcgg gtcaccgggc
     3361 gactgagtgc ggtaggatgc gttcatgacg gaacttgcaa tacgcccgga gttgctccgc
     3421 gaccttccgg aagacaaaca gcgccaactg cgcgactacc gggacctgct ctttcgctgg
     3481 aatcagcggt tcaacctgac ggccatccgc gaaccggaag cgatcgacgg gcgactgatc
     3541 gccgatgccc tgcgccttct gcccacgatc gacgaggagc tcgcgcgaca ggacggaaaa
     3601 cttcgcctga tcgatctcgg caccggcgcc gggctgccgg gcctggtcct gaaaatcgcc
     3661 cgccccgaga ttgcgatcgt gctcgccgat gcgaccaaca agaagatcca gttcgtccag
     3721 tacgtcgtca ccgagctcgg cctgacctcc gcccggaccg tccatggacg cgctgaagac
     3781 atcggtcacc acctcgatta ccgcggccag ttcgatctcg tgaccgcgcg cggcgtggca
     3841 tcattgccga ccctgctcga gttgacggtg ccactgctcg tagtcggcgg cgcctttatc
     3901 ttccccaagg gccaggatct ggacgacgag ttggaaatgg ggcaacgggc agccaaagcc
     3961 ctcggcgcgc gcatcgagtc ggccgatttg ttgcccggtg acacaagcga acctgttacc
     4021 cggttggtga ggggcgttaa gattgcctca actcctggac gctacccacg acgggcgggc
     4081 atccccaata aggaaccgct tgggagggac ggccgatgac caagagccgt attttttacc
     4141 gtaaagagaa gctccgcgga gcctgctggg aataccagat cgacggcggc tggctgacgg
     4201 tgctcggcga cgaagagcgg cacattctcc accaccgctt cagcttctac cgcccgcgcg
     4261 aagtcgaggt cctgcgggcg gacggaccgg tcacgctcgt ccgctggggc gccgagcaat
     4321 ggttcttccc tgaccggtgg ttttccctcc tccggttcgt caccgaatca gacgacacgg
     4381 tcggctatta cgtcaacttc tcccggccgc tcaccgagct gaaggccaat tactaccagg
     4441 atctcgatct cgagctcgac ctctggcttc accccaatgg ggttgccacc gagctggacc
     4501 gcgacgagtt caaccaggag atcgagtgtg cccgcctccg gccggattgg gccgcccagg
     4561 tgatcgaggc gctcgggcag gtctcctccg cggtctcgtc gtccctcgcc gagttcggtc
     4621 ccaatctcga cgcgcaccgg gatccggcaa acggcctccc gatcttcatc ctccgcgcct
     4681 agacgtcctc cgggcggagc gcgaccggtt tcggcggctc ccgcttgatg ccgatgctgc
     4741 ggtcgtaggc aagccgtcgt ttcggatccg ccaacacatc cttcgcctgc agcacctcgc
     4801 gcattttggc ggccgcttca ggatccttgc tgcgatcggg gtggtacttc attgccagtc
     4861 gcttgaacgc tgcctcgatc acgtcaggat cggcatcctg atcgacctgc aacgtctgat
     4921 agagcgtccg gaccggccgt gcttcccgct cgctttgatc cggcagctgc tcgaccagat
     4981 cagccaaccg atcggccgta atcagccgcg cctgcaactg gtccttcatc tctgggggaa
     5041 tcgcgaaccg cgcctggcaa tgccggcatt cgagcacgtg ctgctcgccc atcggcaggg
     5101 ctgcaaacat cgagatcgtc atccgccgct tggcgagata gaccgtgaac gagcccgtct
     5161 tgccgcactt cggacacacg ccctggacgt agccgaggtg cgcgatatcc tcgcggctac
     5221 ccagaaacat gaccgcgcct cgtcaccgga tcaaccacca ccgcccgcac tgcccttgat
     5281 gccgccgtcc gccagatcgg ccggatcgag caacctgtcg agctcgtcgg gcgagagatc
     5341 ggtcatctct gccgcgacat ccttgacccg gcggttctcg gcataggcac gcttggcgat
     5401 cttcgcgccc aggtcataac cgatgatcga gttcaacgcc gtgaccatga tcgggttctt
     5461 gtcgacgagg tcggcgatgt gctgccggtt gatcgtgaat ccggccaccg ccttgtccgc
     5521 gagcacgttc gaggccgacg cgaggagctc gatcgactgc agcaggttgt gcgcgatgac
     5581 cggaagcatc acgttgagct cgaagttgcc catctgcgcg ccgatcgtca cggtcagatc
     5641 attcccgatc acctgcgcgc agaccatcat cacggcctcg ctgatcaccg ggttgatctt
     5701 gcccggcatg atcgacgatc cgggttgaag cacaggcagc acgatctccc ccagacccgc
     5761 ctgcggtccc gagttcatgt gccgcaggtc gttggcgatc ttcatcaggc caaccgcgta
     5821 cgctttgagc tgaccactca gctcggcggc cgtgtcctgc gttgcctgca gggcgaaatg
     5881 gtcccgtccc tcgacgaacg gcagaccggt tcgctgggca agtgccgccg caaccttcgc
     5941 cccatactcc ggatgcgcat tgatcccggt cccgaccgcg gtgccgccga gcgccaactc
     6001 ggcaagtcgc ggcagggtgg actcaatccg ctcccgggcc tgcgccacct gcgtcgccca
     6061 accaccaatc tgctgcccga gccggatcgg catcgcatcc atgaggtgcg tgcgaccggt
     6121 cttgatcaca tcgtgggttt cctcggcccg gcgccgcagc acgagctcga ggtggtccag
     6181 tgccgggatc agtgactcgc gaatgagcag gaacgccgcg acatggatcg tggccgggat
     6241 gacgtcattg ctgctttggc acatgttgac gtcgtcgttc ggatgtaccg gccggccacc
     6301 gcccgcgatc tggactgccc gggtcgcgat cacctcgttg gcattcatgt tggtcgaggt
     6361 accggaaccg gtctggaaga tgtcgatcgg gaattccgcg tcccagcgac cgtcgacgac
     6421 ctcctgggcg gcctggacga tcagcgacgc gcggtcttcg ggcaacaacc cgagatcgcg
     6481 attggcctcg gcagccgcct gcttgatcag ccccaacgcc cgaatgaacg agcgcgagaa
     6541 tgcgatgccg ctgatcggga agttctcgac cgcccgagcc gtcgtggcgc cgtagagggc
     6601 atcggcgggg acggtcatct cgcccatgct gtcacgctcg gtgcgggtgg cagcgggact
     6661 ctggtgctcg ctcatcgtcg cggtcgttcg ctttccgttg ggttcctgtt cagaaatccg
     6721 aatctcaacg cgttgattgt accgcccacc cgggacggct ccgcgggcat gagtggggag
     6781 ggatatgttg accgtcaaat ctgaaggtaa ccgtattcag cggcgagact ggttgcgtca
     6841 tccgccaaca acagactacc gatgatcgaa tcttcttacc ttggcaaatc tgtgttgaca
     6901 aagagtccat ggcgggcgta ggatgcgaag acgttgggga tataacgaaa tgtcgccgcc
     6961 gagggtgccg tgaggtacct cgtggtcctc ttgcgcgcga catggagggt gtcaggtgca
     7021 gaacccggcc tgctattcgt gtcgtttctt tcaagccggg tcgacgaacg cgttcgggtc
     7081 ctgcaatcat ccaaaccacc agcaatccgg tgtcccgatc accattcgcg cccaggaagt
     7141 gaactgctac cgtggcttcg gcacatccga ctggatgccg gccctgattg gcgattccgc
     7201 cgcctgcgag gacatcgtcc tcagcgagcg tccggccccg taccgcgcac cgtggcacgg
     7261 cctgaccccg atcgagcaga ccgagacacc cgcgtacggc ctgcgcgact gatcacccgc
     7321 cggtatccgc caacgcgcgc ctcaggaacg ggctgacgat cagccccagc gcggccagga
     7381 taccgagcac ccagaatgac cgcgcgatgc cgatctcgtc gccgagcgcg ccgatcgcga
     7441 acggtgctgc cgcgatcgcc acgccgccgg ccatgtagag cttcgccaca atccagtcgg
     7501 tcgcgtccgg gaatgcccgt gcggccaggg tgatgatcat cgggaacgtg atcgcgatcc
     7561 cgaaacccga gagcagcagt ccgaccacct tgaataccgc cgtcggtccg cccgcaagca
     7621 acaggaagcc ggccagcgcc atcgcgaacg aggccagcag caacctggac gcggcatagc
     7681 ggcgcaccgc ccacatcagc ccgacccggc agacaaccat gccggcatag aagacggtca
     7741 tcaatgtcgc cgccgtgccc ttgctgaagc cgcctgcctt gttcaggaac tcggccgctt
     7801 gatatccata ggaccactcg aatcccgcct ggcaaacgat cagcaggcag aacagccatg
     7861 ccagacccgt catcttccgc gcggcgccct tcgttcgcgg tctcgacgca tccgaaaact
     7921 cgagaccgcg gagcggaatg aatgccgaga tcgcggcaag cacagggatc aggagcgcga
     7981 tctgccacga atccgtcagc gccacgatcg gtccgactcc gagcgtcgtt gccatcaagg
     8041 cgacgctgac gacgaggttc agttccgcca ttgctgtggc ctgatgcgcc gggtgtcgat
     8101 ccagcacctc ggtctggctc aggatgatcg cgaacggccc ggcaaggcca atcaccgccg
     8161 cggccggcaa ggtcagcgct acactcggcg ccaggcagag cgcaagcaac ccaaacgaaa
     8221 cgacggcgac tgatgcaagg caggtcgcgc ggcgcccaaa ccgggcggct gccggcgcca
     8281 cggtgctcga ggcgagcatt ccgcagaccg ccatcaccgt gaaatggagc ga
//
LOCUS       DATAOZ010000643         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8407714, whole genome shotgun sequence.
ACCESSION   DATAOZ010000643 DATAOZ010000000
VERSION     DATAOZ010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8407714"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1013
                     /locus_tag="VHR64_16135"
     CDS             276..1013
                     /locus_tag="VHR64_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304416.1"
                     /translation="MVSREQIQINGAPTRIHGSFDREVQDESGQTVGEMEVVAIIRGR
                     LQNKQFMQLISRETVQLDYQDGQTSVTMVTRVVNHSAVASGEGEGTVYRHDITFREQP
                     ESYQRRAAALEAAAALRAKTAPRPSPRIAVQEREPVGDISDVTAATDPSSWGDAIRQL
                     RGSMPKPQAPEEPLTMPELAGIETVLVSLRLEALIHVLESTGCIPRGAAEIQFRELLE
                     ERFFEDASQIVGERVAQRALREMHTSH"
     gene            1233..3188
                     /locus_tag="VHR64_16140"
     CDS             1233..3188
                     /locus_tag="VHR64_16140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX3304417.1"
                     /translation="MAQSLQASLRLPVTKPAEPARSLRAFLSEYILVILAASTWLLLI
                     SAAALDHLTSTPQSVVSALYVGAYVTGGTLAVKAAITDLMHGIVNVDLLMVTAAAGAA
                     VINAWGEGGVLLALFASSNALEHFAMDRTRNAVRALMALAPEQATVLRDGHEQVLRIE
                     DVLVGDQVVIRPGERVPVDGIVHTGASDIDQSTITGESVPVGKTIGDTVFAGTMNGYG
                     MLQVEVTKHATESTLARIVRVVSDAREQQGRTQRFAEAFEGKYAVGVILFSALYFLFT
                     WQILDYVAHDAFYRAMTILVVMSPCALVISTPASTLSALANAARHGILFKGGAYLEAA
                     GSIPIIAFDKTGTLTLGKPTLTAAISLDPALTDDELVQLAASVERFSEHHIAKAILTE
                     TKRRGLPLLTAENFVAKPGHGVSAEVDGHLYWVGNATLMREIGIQTTIDHTDGRKLLE
                     SGKTVVFVTDECRVLGLIEISDKIRPEARSIIERLKRVGIEKVVMITGDNERVAAEVA
                     REVGITDIRADVLPEAKMDVVAELKREGAVAMVGDGVNDAPALATAELGIAMGGAGTD
                     VALETADMVLMTDDLNGVAYAIELSRRTHRTIIQNIAFSLSVIVVLAIAALTIGIPLP
                     LGVVGHEGSTVIVVLNGLRLLTWGRES"
BASE COUNT          643 a         1054 c         1201 g          605 t
ORIGIN      
        1 tcgaacggcg tgtattcgga ttcgaggttg agcggatcgg cagcgttgag gcgagggggc
       61 gttggcatgc tcacgagcgc ttccctgatt cagcagatgg acgtgcggca agtgtagggg
      121 aggtcgccga agcggtcctg aatgcccggc cggcgtgacc agaacagccg gagcagccgg
      181 tacagatggt ggactgtccg gacgcggccc gatccatagg atgaatggac ggggtccgcc
      241 gggcggcggc ggtgcgtatc gcaagagggg caggcgtggt ttcgcgggag cagattcaga
      301 tcaacggtgc gccaacccgc atccatggaa gtttcgatcg cgaggttcag gacgagagcg
      361 gccagaccgt cggcgagatg gaggtggttg cgatcatccg cggacggctc cagaacaagc
      421 agttcatgca gctgatcagc cgggagacgg tccagctcga ttaccaggac ggccagacat
      481 cggtgacgat ggtcacccgg gtcgtcaacc actcagcggt ggcgagcggc gagggcgaag
      541 gcacggtcta ccggcacgac atcacgttcc gggagcagcc ggagtcgtat cagcggcggg
      601 cggctgcgct cgaggcggcg gcggcgctca gggccaagac agcgccgcgt ccatccccac
      661 ggatcgcggt ccaggaacgg gagccggttg gtgatatttc ggatgttacg gcggcgacgg
      721 acccgtcgag ctggggcgat gcgatcaggc agctcagggg cagcatgccg aagccgcagg
      781 cgccggagga accactgacg atgccggagc tggccgggat cgaaacggtg ctggtcagct
      841 tgcgcctgga ggcgctgatc catgtgctcg agtcgaccgg gtgcatcccg cgcggggcgg
      901 cggagatcca gttccgggag ttgctggaag agcgcttctt tgaagacgca agccagattg
      961 tcggcgagcg ggtcgcccaa cgcgccctcc gggagatgca cacgagtcat taggcttgag
     1021 acgtgagccg gtggatggtg gttggttgtt ggtggatgga tatagacaac tatttccaac
     1081 caccatccac taaccaccag agaccgaagt ctgacgacgg aagtcagttt tgagaatgaa
     1141 tctcattatc gggtataatt gagcagcggt tcggcggggc cggatcgctt cgttctcgct
     1201 cgcaactcgc accccgctcg atcggatcat tcatggcgca atcgctccag gcctccttgc
     1261 ggctcccggt gaccaaaccg gccgaacccg cacgctcgct ccgggcgttc ctttccgaat
     1321 acattctcgt catcctcgct gcctcgacct ggctcctgct gattagcgcg gcggcgctcg
     1381 atcacctgac ctccactccc caatcggtcg tcagcgcgct ctatgtcggc gcgtacgtga
     1441 ccggcgggac cctggccgtc aaggcggcga tcaccgatct gatgcacggc atcgtcaatg
     1501 tcgatctgct gatggtcacg gcggcggccg gcgctgcggt catcaatgcg tggggcgagg
     1561 gcggcgtgct gctggccctg ttcgcctcat caaacgcgct cgagcacttc gcgatggacc
     1621 gcacccgcaa cgccgtccgc gccctgatgg ccctggcccc ggagcaagcg actgtcctgc
     1681 gcgatggtca tgagcaggtg ctccggatcg aggatgtgct ggtcggcgat caggtggtga
     1741 tccggccggg cgagcgcgta ccggtcgacg ggatcgtcca caccggcgcg tcggacatcg
     1801 accagtcgac gatcaccggc gaatcggtcc ccgtcggcaa gaccatcggc gacaccgtct
     1861 ttgccggcac gatgaacgga tacggcatgc tgcaggtcga agtgaccaag catgcgaccg
     1921 agagcacgct ggcccggatc gtccgcgtcg tcagtgatgc gcgcgagcag cagggtcgga
     1981 cccagcggtt cgcggaggcg ttcgagggca agtacgcggt cggcgtgatc ctcttctcgg
     2041 cgctctactt cctgttcacc tggcagatcc ttgattacgt cgcacacgac gccttctacc
     2101 gggcgatgac gatcctggtg gtgatgtcgc cctgcgcgct ggtcatctcg acgccggcct
     2161 cgaccctctc cgcgctggcc aatgcggccc ggcacggcat cctgttcaag ggcggcgcgt
     2221 atctcgaagc ggccgggtcg atcccgatca tcgccttcga caagaccggc accctgacgc
     2281 tcggcaagcc gacgctgacc gccgcgatct cgctcgatcc ggctctgacc gacgacgagt
     2341 tggttcagct tgcggccagc gtggagcgct tctccgaaca ccacatcgcg aaggcgatcc
     2401 tgacggagac caaacggcgc gggctgccgc tgctgacggc cgagaacttc gtcgccaagc
     2461 cgggtcacgg cgtctcggcc gaggtcgatg gacacctcta ctgggttggg aatgccacgc
     2521 tgatgcggga aatcggcatc cagaccacga tcgaccatac cgatggccgc aagttgctcg
     2581 agagcggcaa gacggtcgtc ttcgtgaccg atgagtgccg cgtgctgggg ctaattgaga
     2641 tctcggacaa gatccggccc gaggcgcgct caatcatcga gcggctgaag cgagtcggga
     2701 tcgagaaggt cgtgatgatc accggcgaca acgagcgtgt cgcggccgag gtcgcgcgcg
     2761 aggtcgggat caccgatatc cgggccgacg tgttgcccga ggcgaagatg gacgtcgttg
     2821 ccgagctcaa gcgcgaaggc gcggtcgcga tggtcgggga tggcgtgaat gatgcgccgg
     2881 ccctggcgac ggccgagctg ggcatcgcga tgggcggcgc gggcacggat gtcgcgctgg
     2941 agacggccga catggtgctg atgacggacg acctgaacgg cgtggcctac gcgatcgagc
     3001 tgagcagacg gacgcatcgg acgattatcc agaacatcgc cttctcgctg agcgtgattg
     3061 tggtgctggc gatcgcggcc ttgacgatcg ggatcccgct gccgctgggc gtggtcggcc
     3121 acgagggaag taccgtcatc gtcgtcctga atggcctgcg cctgctgacc tggggccggg
     3181 aaagctaatc gaattgctct ggaacagcag cacgctcatg catgacgcga aggatctcga
     3241 ctgaatctgg gaagacccgg cagtaaacaa catgtttgcc aacgaagtgt cggcgcaggc
     3301 ttgaaacagc ggggtccaca agcgtcccga gctccggaaa ccgccccagc gaatcgaatg
     3361 ttgctggaat agaccgcaca tagcggcttg cctgtgttga gccccagacg tcggctgaat
     3421 attgtcggat cagttgaaga tcgttggttg cgctagcagt aagccgaatc gatcgttttg
     3481 ccacccgatc ctagaactcc ccg
//
LOCUS       DATAOZ010000644         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7354555, whole genome shotgun sequence.
ACCESSION   DATAOZ010000644 DATAOZ010000000
VERSION     DATAOZ010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7354555"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VHR64_16145"
     CDS             <1..361
                     /locus_tag="VHR64_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX3304418.1"
                     /translation="RAWVGARELALRPKEYGLLLALANDAGKLFSRQDLLDAVWGADI
                     FVDERTVDVHVSWLRGKLRRAGMTHDLIQTVYGAGYRLNAETGDHLQESARHDGGGIQ
                     ASRAARFDNAEYKEKSA"
     gene            358..1380
                     /locus_tag="VHR64_16150"
     CDS             358..1380
                     /locus_tag="VHR64_16150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3304419.1"
                     /translation="MNALSSIRTLALTVAVAAAMVLPTLASAQDATPMPYSPGADLGS
                     LSGKISADGSSTVFPIMEALAEEFGKQASGVDITVDFSGTGGGFKRFCAGETDISDAS
                     RPIKDEEMATCAENGVSYYTFEIAYDGIALVVNPENDWVTCLTVDQLHQMWAKDSTVK
                     KWSDIDPSWPSEDIALYGPGTDSGTYDYFTQQINGEEGVSREDYTPSEDDNVLVEGVA
                     GDKNALGFFGLAYYEQNQDRLKLVQVDGGNGCVAPSAETVQNLTYAPLSRPLFLYVSA
                     NSLTRPEVQEFMRFSVAEANVYTEEVGYVDSPLKVYTDDQAKLEAAIAGSAQPDGPAA
                     QGTPTS"
     gene            complement(1651..1992)
                     /locus_tag="VHR64_16155"
     CDS             complement(1651..1992)
                     /locus_tag="VHR64_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304420.1"
                     /translation="METRTMDAHNPFTGRTLRVLRPAQDQATYCGCSRAEVGAAQAQW
                     DDAERIRIEELSRITMLEREVQAEEPASKAHENRLRVAFLSIRGNTMAMWATQRDTIL
                     AGGDPNLPTLA"
     gene            complement(2179..2415)
                     /locus_tag="VHR64_16160"
     CDS             complement(2179..2415)
                     /locus_tag="VHR64_16160"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX3304421.1"
                     /translation="MSTGRITAIRADKGFGFIKDGPDASNSNDIFFHRSAVVGVDFED
                     LREGQEVSYRTEPDPRDASRFRAADVRVVSAESN"
     gene            2815..3030
                     /locus_tag="VHR64_16165"
     CDS             2815..3030
                     /locus_tag="VHR64_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304422.1"
                     /translation="MGNSRYSAEDRKANQDAHEARMAQFEKSEAGARARREQTGEPLG
                     KPESKSEAAEQQAHEQDAAAQPDQRAT"
     gene            complement(3123..3623)
                     /locus_tag="VHR64_16170"
     CDS             complement(3123..3623)
                     /locus_tag="VHR64_16170"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3304423.1"
                     /translation="MTELIDFDGDRHHDELQRLLTAYLEEAVPALMEVGIKEDIPGIV
                     SDDLAHPEKFSPPNGRRLLVVDDGAVLGCGAMRVIGPGVAEIKRMYLQPEARGRGPGR
                     MMIDGLLDSARDMGCHEARLDSGWFMTDAHRLYRAAGFTACDPYPESEIPPDFDARWV
                     YMRRDL"
     gene            complement(3660..4280)
                     /locus_tag="VHR64_16175"
     CDS             complement(3660..4280)
                     /locus_tag="VHR64_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304424.1"
                     /translation="MVRAQLVLSLVIAFALSMFAVPVRAQSEGAETLQSVGQSASAGF
                     NRTDGCITTSVIVLAGQQISGPGRTTFAVVLIDSRNTCTDTLLVFGGGTASGVNFRMD
                     PQLKKAAVHASVPFDDIVSNSTFNVQVDMVWTGTGALERGQDQFTFEDEGFTIIAHSV
                     GAARDATASGAVASASTTFVPAGSTAPGFLFWFNQGQVIISPHPSQ"
     gene            complement(4401..>4919)
                     /locus_tag="VHR64_16180"
     CDS             complement(4401..>4919)
                     /locus_tag="VHR64_16180"
                     /inference="COORDINATES: protein motif:HMM:NF027856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304425.1"
                     /translation="LRIAPFHLLASEGQVHTNRDHGWHMTTLARLCAADPELLMATAH
                     RVIELADEQSVAAATDWWLELTRQGGEGIVVKPWDFIARGEKGLVQPAIKVRGREYLR
                     IIYGPEYTLPEHMERLRSRGLSAKRSLALREFALGLEALNRFVNRESLRRVHECVFGV
                     LALESEPVDPRL"
BASE COUNT          943 a         1573 c         1576 g          827 t
ORIGIN      
        1 ccgcgcctgg gtaggcgcgc gcgagctggc gcttcgcccc aaggaatacg gtctgctgct
       61 cgcgctggcg aacgatgccg gcaagctctt ctcccgccag gacctgctcg atgcggtctg
      121 gggcgcggac atcttcgtgg acgagcggac ggtcgatgtc catgtctcct ggcttcgcgg
      181 caagctgcgc cgggccggaa tgacacacga cctgatccag acggtctacg gcgccgggta
      241 ccgcctgaac gcggagaccg gagatcacct tcaggaatca gcccgccatg acggcggcgg
      301 cattcaggcg agccgggcgg ctcgcttcga taacgcggag tacaaggaga aatctgcgtg
      361 aacgcattga gctcgattcg cacgctcgcc ctgaccgttg cggtcgcggc ggcaatggtc
      421 ctgccgaccc tggcatcggc ccaggatgcg acccccatgc cctactcgcc gggcgccgac
      481 ctcggctcgc tgagcggcaa gatctcggcg gacggttcgt cgaccgtctt cccgatcatg
      541 gaggcgctgg ccgaggagtt cggcaagcag gctagtggcg tcgacatcac ggtcgacttc
      601 tcgggcaccg gcggcggctt caagcgcttc tgcgccggcg agactgacat ctcggacgcg
      661 tcccgcccga tcaaggacga ggagatggcc acctgcgccg agaacggcgt gagctactac
      721 acgttcgaga tcgcctacga cggtatcgcc cttgtggtca acccggagaa cgactgggtc
      781 acctgcctga ccgtggatca gctccaccag atgtgggcca aggactcgac cgtcaagaag
      841 tggagcgaca tcgacccgtc atggccgagc gaggatatcg cgctctacgg ccccggcacc
      901 gattccggca cctatgacta cttcacgcag cagatcaacg gcgaagaggg cgtctcccgc
      961 gaggattaca cgccgagcga ggatgacaac gtgctggtcg aaggcgtcgc cggcgacaag
     1021 aacgcactcg gcttcttcgg tctcgcctac tacgagcaga atcaggatcg cctgaagctg
     1081 gtccaggtcg acggcggcaa cggctgcgtc gcgccctcgg ccgagacggt tcagaacctg
     1141 acatacgcgc cgctctcccg cccgctcttc ctctacgtca gcgccaacag cctgacccgg
     1201 ccggaagtgc aggaattcat gcggttctcg gtcgccgagg ccaatgtcta caccgaggaa
     1261 gtcggctatg tggactcgcc gctcaaggtc tacaccgacg accaggccaa gctcgaggcg
     1321 gcgatcgccg gatcggccca gccggacggc ccggcagccc agggcacgcc gaccagctaa
     1381 ccaaccacgc aagactcccg tattccaaga cacccggacc gcgaggcttg tcgcggtccg
     1441 ggtgtcttat ctggaattgg gcttagtgat cgtgaggtcg gccccacgtg gcccaggaaa
     1501 aaccgatgcg cgtcgcggac ggcgtcagta cccttcgttt tacgctgagt ggggaaaaga
     1561 atctgcggta aagcggcgcg cctgacgctg caatcgatag ggcgaccgac actgctccat
     1621 tagcgctggg gaagacgtct gaaccccaca ctaggccagg gtcggcaaat tggggtcgcc
     1681 accggcaaga atcgtatccc gctgcgtcgc ccacatggcc atcgtgttcc cgcggataga
     1741 cagaaaggca acccgcagcc ggttctcgtg cgccttgctc gccggctcct ctgcctgcac
     1801 ctcacgttcc agcatcgtta tccgggagag ctcttcaatt cggatccgct cggcgtcgtc
     1861 ccactgcgcc tgggccgcgc caacctcggc ccgagaacat ccgcaatatg ttgcctggtc
     1921 ctgagcaggg cgcaagaccc gcagcgtgcg gccggtgaag gggttgtggg catccatcgt
     1981 gcgcgtctcc aatgtcgatg tcgttgcgat caggcaaaac agccctgctg cgagcggggg
     2041 atcagagcca ggcggggtcg agcggagtca tggtctggac ggagcgatgt gcacctgttc
     2101 gccacccaca cccgttttca tacaaacgta ggcaccggtc ggcgattcag gcgtcgccga
     2161 ccggagaatc aaggtgcgct agttcgattc ggcactcacg acgcggacgt cagccgcccg
     2221 gaagcgcgag gcatcgcggg gatccggctc agtgcggtag ctgacttcct ggccctcgcg
     2281 cagatcttcg aaatcaacgc cgacgaccgc tgagcggtgg aaaaagatgt cattgctatt
     2341 cgaggcatct ggcccatcct tgatgaaccc gaatcccttg tcggcgcgaa tagcagtgat
     2401 gcggcccgtt gacacggtac tactccttgg tgatgaaatc gagaaacggc ggcccaagtg
     2461 agtcactgtg tcccggtggg gaatcccgca tggactataa caataattat ggtattgtgc
     2521 aagtattaca gatgttatag tcgacctatc acataagggg tgccagcatg aagacgattc
     2581 gggccctgcg acaagaacgc ggatggacgc agtttcaact ggcgctggag gtcggagtgc
     2641 agccccagac aatctatttt tgggagagcg ggcgtcgcac gccgttggtg ccgcagcttc
     2701 gcaaattggg gcaagtcttc ggcatgtgct ccgatgacat cgatcttgag ccggggccgg
     2761 aacgagtgga ctcgtctcgc gatgggtccg tcaggaccgt ggaggcaatc tgacatggga
     2821 aacagcaggt acagcgccga ggatcgcaag gccaaccagg atgcccatga ggctcgtatg
     2881 gcccaattcg aaaagtcgga ggccggcgcc agagcgcgtc gcgaacaaac cggggagcca
     2941 ctgggcaagc cggaaagcaa gtcggaagct gccgagcaac aagcgcacga gcaggatgcc
     3001 gcagcccagc cggaccagcg agccacgtag cctaatcccc acatcgatcg gcaatggctc
     3061 ggccggggaa gatcctcccc ggtactggct cgctcgcgcg ggcgctcagt gcgcgttgac
     3121 agtcagaggt cccggcgcat gtagacccag cgcgcatcga agtcgggcgg gatctcgctc
     3181 tccgggtagg gatcgcatgc ggtgaagccg gccgcgcggt aaaggcgatg ggcgtcggtc
     3241 atgaaccagc ccgaatccag ccgagcttcg tgacagccca tgtcgcgagc ggagtccagg
     3301 agcccatcga tcatcatccg gccggggccg cggcctcgcg cctccggttg caggtacatc
     3361 cgcttgatct ccgcgacgcc gggtccgatc acacgcatgg cgccgcaccc gagcacggcg
     3421 ccatcgtcga ccacgagcag gcggcgacca ttgggcgggc tgaacttctc cggatgcgcg
     3481 agatcgtccg agacgatgcc ggggatgtcc tccttgatgc cgacttccat gagcgcgggc
     3541 acggcttctt cgaggtaggc cgtgagcaga cgctgcaact cgtcgtgatg gcgatctccg
     3601 tcaaagtcga tcagttcggt cattcagttg tctccgtcca atgacagcgc tcgcccggcc
     3661 tactgcgaag ggtgcgggga gatgattacc tggccctggt tgaaccagaa caggaagcct
     3721 ggcgcagtac tgccggccgg cacgaaggtg gtgctcgcgc tggccaccgc gccggatgcg
     3781 gtggcatcgc gggcggcgcc aacgctgtgg gcgatgatcg tgaacccctc gtcctcgaac
     3841 gtgaactggt cctggccgcg ctcgagggcg cctgttccgg tccagaccat gtcgacctgg
     3901 acgttgaacg tcgagttcga cacgatgtca tcgaacggga cagacgcgtg taccgcagcc
     3961 ttcttcaact gcggatccat tctgaagttc acgccggatg cggtcccgcc accgaagacc
     4021 aggagggtgt cggtgcaggt attgcgggaa tcgatcagga ccacagcgaa cgtcgtgcgg
     4081 ccgggcccgg atatctgttg gccggcgaga acgatcaccg aggtggtgat gcagccatcg
     4141 gtgcgattga agccggccga ggcgctctgg ccaacgcttt ggagggtctc ggctccctcc
     4201 gattgcgctc gtaccgggac agcaaacatg ctgagcgcga acgcgataac cagactgagt
     4261 acgagctgag cgcgcaccat ggcgggcctc ccttcgggtt gctcgacgat cacgttatcc
     4321 gcaaatgaag ctgacgcgcc caccctagcg gcaatatttc gccatgtcaa cagacaactc
     4381 gaccgagcga ggccgtcgca tcacaaccgg gggtcgaccg gctcgctttc gagcgcgagg
     4441 acgccgaaaa cgcactcatg cacccggcgc aggctctcgc gattcacgaa ccggttcaac
     4501 gcttcgagcc cgagcgcaaa ctcgcgcagc gcgagcgatc gcttggccga cagcccacgc
     4561 gatctcagtc gttccatatg ctcgggcagt gtgtactcgg gtccgtagat gatgcgcagg
     4621 tattcgcgac cgcgcacctt gatcgccggc tgcacgagac ccttctcgcc acgcgcgatg
     4681 aagtcccacg gcttgacgac gatcccctcg ccgccctgcc gggtcagctc aagccaccag
     4741 tccgtggccg ccgcgacgct ctgctcgtcc gccagctcga tcacccggtg cgccgtagcc
     4801 atcaggagct ccggatcggc cgcacacagt cgcgccagcg tggtcatgtg ccagccatgg
     4861 tcacgattcg tgtgcacctg gccctcgctg gcgagcagat ggaacggcgc gatgcggag
//
LOCUS       DATAOZ010000645         6186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1036228, whole genome shotgun sequence.
ACCESSION   DATAOZ010000645 DATAOZ010000000
VERSION     DATAOZ010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036228"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VHR64_16185"
     CDS             complement(<1..770)
                     /locus_tag="VHR64_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX3304426.1"
                     /translation="MTTKTVSPVTKSLEIAGRTLTMEHGLIAEQASGAVTMRYGDTML
                     LVTVVGAREPKENAAFFPLSVDFEERMYAAGKIPGGFIKREGRPTENAILSARLTDRP
                     IRPLFPKGYMSEVQVVSTVLSADQENDPGILSIIGASAALSISPIPWEGPVGAARIGL
                     VDGNLVINPTFSDLENSTLDMVIAGTADAIVMVEGEAGEISEEQLLEGMMLAHDEIKR
                     ICAFQQELAAEVGQDKWEFTAPARDETLMDDVRGFLGNR"
     gene            complement(1100..1369)
                     /gene="rpsO"
                     /locus_tag="VHR64_16190"
     CDS             complement(1100..1369)
                     /gene="rpsO"
                     /locus_tag="VHR64_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX3304427.1"
                     /translation="MALVKEQKSEIISQFRVHDADTGSPEVQVAILTERINQLTDHLR
                     SNKHDHHSRRGLLQMVGKRRRLLAYMNKKDPERYRTAIARLGLRK"
     gene            1481..2344
                     /gene="mtnP"
                     /locus_tag="VHR64_16195"
     CDS             1481..2344
                     /gene="mtnP"
                     /locus_tag="VHR64_16195"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX3304428.1"
                     /translation="MTKLGVIGGSGLYDIEQMTDVRRERVQTPFGDPSDNLIFGRIGE
                     QEIVFLPRHGVGHYLNPSEVPYRANIHAMKQLGVTHLLSISAVGSLTERYPPLTLVCP
                     DQIIDRTITRDRTFFDCGVVAHVGIAHPFCADFTRHVIERAAGAEVRVEQGGTYVAIE
                     GPQFSTIAESELFRTWGASIIGMTAMPEARLAREAELHYVQLAMVTDFDVWHESEGHV
                     SVEKVVGYLRRNAENAKQVVLAVLNGGPVEPASCGCATALQNAIITAPDRIPAEQRDR
                     LSIIAGKYLQS"
     gene            2372..3670
                     /locus_tag="VHR64_16200"
     CDS             2372..3670
                     /locus_tag="VHR64_16200"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX3304429.1"
                     /translation="MSLTLFAAALLICGYAASWANERSIATVSTGQLQSAALLTGALL
                     LVPIVWSVARMAGDDIIFPVCALLLAAGAVMMHRLQPDIGGPDSNLGNLSGRHTIYVL
                     LSIVVMVITGRWFPFWSFLRRYKYLTVVGSIGLLFVTLVAGRERYGARLWLGVGSFEI
                     QTSELIKIGLILFLAAYLHEKHEMMHGRWQLWRLSLPPIPYLIPLIVLLVVALLMVVA
                     MNDLGTALLLFSTALAMLYVALRKISYVVISLAAFFAGSAFAYVTFDRVGVRIQNWLD
                     PWRDPFVSGYQQIQADYAMSSGGMFGTGLGRGEPWRIPAVHTDYIFAAIVEEWGAIGG
                     LAIIVLFGVLLFRGLRIAARADSLYDRYLAVGLSASITIQAIVIVGGVLRMLPLTGVT
                     LPFVSAGGTSLLLNALAIGMLLNISHRQQVGRDWSDHVNT"
     gene            3657..5237
                     /locus_tag="VHR64_16205"
     CDS             3657..5237
                     /locus_tag="VHR64_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX3304430.1"
                     /translation="MSTPEATALVATLVLILGTIWVAARDEGWRRGQGRAADPQRRAL
                     AKLGLVLSIGLIGLAGRLTYVSMIRAKAVRTRTGADANGDVLSNPRVIAAALNAGRGS
                     VMDKSGGVMAETSGEDRRARREFHAPEAAHLLGYFSPLRYGFDGIERSRNEALSGTDP
                     LTVEEAIKSIVRTGAGDGHSVRLTIDPGLQQLAANLLSQTTGSATLIDPRSGRILAMA
                     SSPAYDPNALTTNVQSDIASADAAWTELLANDNRPLLVRPTSGLYPPGSTFKVFTAAA
                     AIDAGAVQPDTVFEDNGSLTVNGRVIPEFNRPDESRTDWTVAEALAWSLNVVYAQIGL
                     TLGSDRFRDAAHRFGIGEAIPFEFPVESGQLAGKNSLDDEVALADTAFGQGELLVTPL
                     HMALVMCAVANGGRIPKPILVDAVLNPDGSTSTRSEPSIWRRPISAQTADTVLSMLYE
                     SVAYGYASRAAIAGLNVAGKTGTAESGRDAPHGWFIGSAGPDAPSVVVSVCLDYGGEG
                     GGLALEIGRELLAASATL"
     gene            5351..5683
                     /locus_tag="VHR64_16210"
     CDS             5351..5683
                     /locus_tag="VHR64_16210"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX3304431.1"
                     /translation="MRFEKLVDRILAQLDPAVRQALDNIVIVVEDSPPDGDPEQFGVY
                     DGVPKSERGGGYTFAVPDRIVIYQKPLEDSFPDPRELEEQIRITLLHEIGHHLGLDEE
                     QLDELGLS"
     gene            5740..>6186
                     /locus_tag="VHR64_16215"
     CDS             5740..>6186
                     /locus_tag="VHR64_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304432.1"
                     /translation="MSEGMSLVLAFPQLIAIPVLLDFYLLVGARISPESMTNRLGNWF
                     IGRSGANSTDAGEWVRGLGDWDMSRLLAFLMPSVIDGMKQSAIERPFDRVSWEPTMPL
                     AIIAAIALLLVGSFIFTAYLVVLASRAEMIRTGPVPFNRLMLNRWGK"
BASE COUNT         1067 a         1977 c         1920 g         1222 t
ORIGIN      
        1 cggttgccaa gaaagccacg gacatcgtcc atgagcgtct cgtccctggc cggcgcggtg
       61 aactcccact tgtcctggcc gacttcggcc gcgagctcct gctggaacgc gcagattcgc
      121 ttgatctcat cgtgcgcgag catcatgcct tcgagcagct gctcctcgga gatctcgccc
      181 gcctcacctt cgaccatcac gatcgcgtca gcggtaccgg cgatgaccat gtcgagcgtg
      241 ctgttctcga ggtcactgaa ggtcgggttg atgaccaggt tgccatcaac caggccgatc
      301 cgggccgcgc cgaccggacc ctcccacgga atcggtgaga tcgaaagggc agccgatgcg
      361 ccgatgatcg aaaggatgcc ggggtcgttc tcctggtcgg ccgagaggac ggttgacaca
      421 acctgtacct ctgacatgta tcccttcggg aacaatggcc ggatcggacg gtcggtcaag
      481 cgcgctgaca ggatcgcgtt ctcggtcggg cgcccctcgc gtttgatgaa accgcccgga
      541 atcttgccgg cagcgtacat tcgctcctcg aagtcgacgg agagcgggaa aaacgcggca
      601 ttctccttcg gctcgcgggc gccgacgacg gtcacgagca acatcgtgtc gccataccgc
      661 atcgttaccg cgccgctggc ttgctcggcg atcaggccgt gctccatggt cagggtacga
      721 ccggcgattt ccagtgattt cgtcactggg ctcacagtct tggtagtcat tgtgctggtc
      781 ttctccacca atgttccgct gccgccgaat tgcggacgag cggcgataga atcgaatggt
      841 ggcgccgggg agaaaggaac tggagtgcgg aggccggaat gacgtgcgcc ctccgcgctc
      901 caatgcctct ctcgccgcat gcgccgggtg cttgtgcaag ttttacgtca gcgggccagc
      961 ctcctttgtc tcatgcgcga cacaacaaaa cccggcgaag gtcgaactcc gccgggaatc
     1021 gcgtggtagt cacgggagtg ttgccgaatc acgccggtca tgtcgcggcg tgatttgagt
     1081 gcttgccggg taaggccgct tatttgcgga gccccagccg agcgatcgcg gtgcggtagc
     1141 gctccggatc cttcttgttc atgtaggcga gcaaccgccg gcgcttgcca accatctgca
     1201 gcagcccacg gcggctgtga tgatcgtgct tgttgctccg gaggtgatcg gtcaactggt
     1261 tgatccgctc tgtcaggatc gcgacctgaa cctcgggcga gcccgtgtca gcgtcgtgta
     1321 cccggaactg gctgataatc tcgctctttt gctctttaac taacgccatt ccggcggagc
     1381 tccctttgcc tcaaaacacg cgtgaatctg ctgcacgctc gaattgtcac agcgaaccag
     1441 tataccatca cgccggtaat ctggatagtg ggggtacctc gtgaccaaac tcggcgtgat
     1501 cggcggcagc ggactctacg acatcgagca gatgacggac gtccgcaggg agcgcgtgca
     1561 gactccattt ggcgatccga gcgacaacct gatcttcggt cgaatcggcg agcaggagat
     1621 tgtattcctg ccgcgacacg gggtcggcca ctatctcaac ccgagcgagg tgccgtaccg
     1681 tgccaatatt cacgccatga agcagctcgg cgtcacgcac ctgctcagta tcagcgcagt
     1741 cggcagcctg accgagcgct accctcccct gacgctcgtc tgtccagacc agatcatcga
     1801 ccggaccatc acccgcgacc gcaccttctt cgattgcggc gtcgttgccc acgtcgggat
     1861 cgcgcacccc ttttgcgctg acttcacccg ccatgtcatc gaacgggcag ccggtgccga
     1921 agtccgcgtc gagcaaggcg gaacgtacgt cgccatcgaa ggtccacagt tctcgacgat
     1981 tgcggaatcg gagctgttcc gcacctgggg agcgtcaatc atcggcatga ccgcgatgcc
     2041 ggaggcccgc ctggcacggg aggcggagct gcattatgtc cagcttgcca tggtgaccga
     2101 cttcgatgtc tggcacgaga gcgaaggtca cgtctcggtc gagaaggtcg tcggatattt
     2161 gcgccggaac gcggagaacg ccaagcaggt cgtgctcgcc gtcctgaacg gcggcccggt
     2221 ggagccagcc tcgtgcggct gcgcgaccgc ccttcagaac gcgatcatca ccgcgccgga
     2281 tcgtatcccg gccgaacagc gggatcgcct ctccattatt gccggcaagt acctgcagag
     2341 ctaaacggtg ttctggcggc tgcgacgaga attgagcctc accctgtttg ccgccgcgct
     2401 ccttatctgc ggctacgcgg cgagctgggc gaacgagcgc tccatcgcga ccgtctcaac
     2461 cggacagcta caatccgccg ctttgctgac cggtgcgctt ctgctggtgc cgatcgtctg
     2521 gagcgtcgcc aggatggcgg gcgacgacat catcttcccg gtatgcgcgc tcctgctcgc
     2581 agccggagcg gtgatgatgc atcggcttca gcctgacatc ggcggccccg attcgaacct
     2641 cggcaacctc tcgggacggc acacgatcta cgtcttgctc tcgatcgtcg tcatggtgat
     2701 cacgggccgc tggtttccgt tctggtcgtt cctgcgccgg tacaagtacc tgacggtggt
     2761 gggctcgatc ggcttgctgt tcgtgacact ggtggcgggc cgggaacgat atggcgcgcg
     2821 gctctggctc ggcgtcgggt cattcgaaat tcagacctcc gaattgatca agatcggctt
     2881 gatcctgttc ctggccgcct acctgcacga gaagcacgag atgatgcacg gccgctggca
     2941 gctctggcgg ctctcgcttc cgcccatccc ctacctgatc ccgctcatcg tgttgctggt
     3001 cgtcgcattg ctgatggtcg tggccatgaa cgacctcggc acggcgctgc tgctcttttc
     3061 gaccgcgctg gcgatgctct atgtcgcgct gcgcaaaatc agctacgtcg taatcagcct
     3121 cgccgctttc ttcgccggat cggccttcgc ctacgttacg ttcgaccggg tcggcgttcg
     3181 catccagaac tggctcgacc cctggcgcga tcccttcgtc tccggctacc agcaaattca
     3241 ggccgactat gcaatgagct ctggcggcat gttcgggacc ggcctcggtc gcggcgagcc
     3301 gtggcgaatc ccggccgtcc acaccgatta catcttcgcg gcgatcgtcg aggaatgggg
     3361 ggcgatcggc gggttggcga tcatcgtcct gttcggggta ctgctgttcc gcggactccg
     3421 gatcgcagcg cgggccgatt cgctctacga tcggtacctt gcggtcggcc tttcggcctc
     3481 gatcacgatc caggcaatcg tgatcgtcgg cggcgtgctg cggatgttgc cgttaaccgg
     3541 cgtgacgctg ccgttcgtct cagccggcgg cacgtcgttg ctgctcaacg cgctcgcgat
     3601 cggcatgctg ctcaacattt cgcaccgcca gcaagttggc cgcgactgga gcgaccatgt
     3661 caacacctga ggcaacagcg ctcgtcgcca ctttggtgct gattctcggc acgatctggg
     3721 tcgccgcacg tgatgaagga tggcgccgcg gccaaggacg tgccgcggat ccgcaacgac
     3781 gcgccctcgc gaaactcggc ctggtgctgt cgatcggcct catcggattg gccggccgcc
     3841 tgacgtacgt gtcgatgatc cgggccaagg ctgtccggac ccggaccggt gcggacgcga
     3901 atggcgatgt cctctcgaat ccgcgcgtga ttgcggctgc gctgaatgcc ggccggggca
     3961 gcgtgatgga caagtccggc ggcgtcatgg ctgaaacgtc gggagaagac cgccgtgcgc
     4021 gccgcgaatt ccacgcgcct gaggccgccc acctgctcgg ctacttctcg ccactccgct
     4081 acgggttcga cgggattgag cgcagccgga acgaggcact ttccggtact gatccgctga
     4141 ccgtggaaga ggcgatcaag agcatcgtcc gcaccggggc cggggatggc cactcggtcc
     4201 gtttgacgat cgacccgggt ctgcagcagc ttgcggctaa tctgctgtcc cagacaaccg
     4261 ggtccgcaac cttgatcgat ccgcgctcgg ggcggatcct ggcaatggct tcaagtcccg
     4321 cctacgaccc gaatgcgctg accacgaatg tccagagcga tatcgcaagc gccgacgccg
     4381 cctggacgga gctactcgcc aacgacaacc gtcccttgct cgtgcggccg acatccggcc
     4441 tctatccacc cggctcgaca ttcaaggtct tcactgcggc ggcggcgatc gatgccggcg
     4501 cggtccagcc ggacacggtc ttcgaggaca acggatcgct gaccgtcaac ggtcgcgtca
     4561 ttcccgaatt caaccggccg gacgaatccc gtacggactg gacggtcgcc gaagcgcttg
     4621 cctggtcgct gaacgtggtc tacgcccaga ttggcctgac gctcggctcg gatcgtttca
     4681 gggacgccgc acaccggttc ggcatcggcg aggcgatacc gttcgaattc ccggtcgagt
     4741 caggtcagtt ggctggtaag aactcgctcg atgacgaagt cgcgctcgcc gataccgcgt
     4801 tcggccaggg cgagttgctg gttacgccac tccacatggc acttgtcatg tgcgcggtcg
     4861 cgaatggcgg tcggatcccg aagccgatcc ttgtcgacgc ggtgttgaat ccagacggca
     4921 gcacgagcac ccgtagcgag ccgtcgattt ggcggcgtcc gatcagcgcg caaacggctg
     4981 acaccgtgct ctcgatgctc tatgagagcg tcgcctatgg ctacgcatca cgcgccgcca
     5041 tcgcgggtct gaacgtggcc ggcaagaccg ggaccgccga gtccggacgt gacgccccgc
     5101 acggttggtt catcggctcg gccgggccag acgcaccctc tgtcgtggtc tccgtatgcc
     5161 tcgattacgg gggcgagggg ggcggtctcg cactcgagat cggacgggag ctactggccg
     5221 cgtcggcgac cctctgaagc atcgtacgta cgcaccaaat ccggcttgtt agcgtgggca
     5281 aaatatccgc aaaagtggta caatttagcc tatgcggaca gccgcctcag cgctcaagcg
     5341 atcccgtcga ttgcgattcg aaaagctcgt cgacaggatc ctggctcagc ttgatccagc
     5401 ggttcgccag gcactcgaca acatcgtcat cgttgtcgaa gattcgccgc cggatggaga
     5461 tccggaacag ttcggcgtgt acgacggcgt gccgaagtca gagcgtggcg gtggctacac
     5521 cttcgcagtt cctgaccgaa ttgtgatcta ccagaaaccg ctcgaagatt ctttcccgga
     5581 cccgcgggaa ctcgaagagc aaatacggat cacgctcctc cacgagatcg gccaccacct
     5641 ggggctcgat gaggagcaac tcgatgagct tggattgtcc tgatgctgga acgaaccaca
     5701 ataacccagc cgaatcgacc tcccgggata ctcgaacact tgtccgaggg catgtcgctt
     5761 gtactggcgt tcccgcagct gatcgcgatc ccggtcctgc tcgattttta tctcctggtt
     5821 ggcgcgcgaa tctcgcctga atcgatgacc aaccgcctcg gcaactggtt catcggacgt
     5881 agcggcgcga actcgaccga tgccggtgaa tgggtgcgcg gcctgggcga ctgggacatg
     5941 tctcgccttc tcgcctttct gatgccgagc gtcatcgatg gcatgaagca gagtgcgatc
     6001 gagcgaccgt tcgaccgcgt ctcgtgggag ccgacgatgc cgctggcgat cattgctgct
     6061 atcgcattgc tcctggtcgg ctcgtttatc ttcaccgcgt atctggtggt gctggcgtcc
     6121 cgcgcggaga tgatccggac cgggccagtg ccgttcaatc ggttgatgct caatcgctgg
     6181 ggcaaa
//
LOCUS       DATAOZ010000646         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8143285, whole genome shotgun sequence.
ACCESSION   DATAOZ010000646 DATAOZ010000000
VERSION     DATAOZ010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8143285"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..746
                     /gene="pdxH"
                     /locus_tag="VHR64_16220"
     CDS             111..746
                     /gene="pdxH"
                     /locus_tag="VHR64_16220"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX3304433.1"
                     /translation="MSLQDLRKDYTLAGLLESDLDPDPFAQFRVWFAEAQEAKGIEPN
                     AMTVATADASGNPGARTLLLKGMDERGFIFYTNYESAKGRNLTDNPRVELLFYWPELE
                     RQVRIHGAVEQLERSASEAYFRSRPVGSQLGALASAQSTVVPSREYLEATYAELEQRY
                     ANEPVPLPDYWGGYRVVPEWIEFWQGRQSRMHDRLRYRRNGEGWTVERLAP"
     gene            complement(816..1373)
                     /locus_tag="VHR64_16225"
     CDS             complement(816..1373)
                     /locus_tag="VHR64_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304434.1"
                     /translation="MATFAELRDSTIAELGEVLGRIDPDAYETFCATLAGANRVVCFG
                     LGREGLALRGFCMRLAHMGFDAHMAGDVTAMPVGTGDVVLITSGPGDLTLTRSMIELA
                     HRAGASAVVVTAQPDAPDPQSADFALTIPAQTMANDRGSASVLAMGTAFELAMAILFD
                     LAVIRIQALRGESLESMRERHFNLE"
     gene            1507..>2656
                     /locus_tag="VHR64_16230"
     CDS             1507..>2656
                     /locus_tag="VHR64_16230"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX3304435.1"
                     /translation="MPDPIETEAQESGAAAVEETTSPAPKRQRKPKAQKRTRSERPNG
                     LMLPTLIVDDMMVLPNLTVPFPVEDEEAAYVFERACRASKRLVFLVCERPIQLPEQHV
                     DGVADPSRIKEFFASFEQDVAEAEVNGWVGEPMNGDSSDYEICPVGIIAEVGQFGPPG
                     RVPQVMLQGISRGIVLQVAQEEPMMVCEVIAHEDPIPAKSLSEAPMAALIEQIENYVS
                     MHPGAPEDVLTMLEGIEEPGHLADLVAISPEFTPLQRQQIVEMLDPLDRLRAVSVIVE
                     KRLMVLSLRNAIVADAQSGLDKQQREYFLREQIRSIQKELGEGTAEETLAKDLRQRVE
                     EAGMPEEIKAKTLDQVDRLEQQHPFSPEIGVIRSYVEWLIELPWSTERS"
BASE COUNT          494 a          844 c          868 g          450 t
ORIGIN      
        1 cggggtacct gacgctcgac gatatcgacg tcgcggccgt ctctgccttc ggggatactt
       61 ccggcaggcc gacgaacgcg ccagccattg ctgcttcagg gtgagtcacg atgtcgcttc
      121 aggatcttcg caaggattac acgctggccg gtttgctcga atccgatctc gatccggacc
      181 ctttcgccca gttccgtgtc tggttcgcag aggcgcagga ggccaagggg atcgagccga
      241 acgcgatgac ggtcgcgact gccgacgcaa gcggcaatcc cggtgcccgc accttgctcc
      301 tgaagggcat ggatgagcgc gggttcatct tctacacgaa ttacgagagc gcaaagggcc
      361 ggaacctgac cgacaacccg cgcgtggagc tgctcttcta ctggccggaa ctggagcggc
      421 aggtccgcat ccacggcgcg gtcgagcaac tggagcgatc cgcctcggag gcatattttc
      481 ggagtcgccc ggtcggcagc cagcttggcg cattggcgtc ggcccagagc acggtcgttc
      541 cgagccgcga atatctcgag gcgacctacg ccgagctcga gcagcggtac gccaatgagc
      601 cggtgccgtt gccggattac tggggtgggt accgggtcgt gccggagtgg atcgagttct
      661 ggcagggacg tcaaagccgg atgcacgatc gcctgcgcta ccggcggaat ggcgagggct
      721 ggaccgtcga gcggctcgcc ccataacctc gcccagtgtc acttccatcc catgaagtag
      781 gacggccccg cgatacgtct agtaatacgt tcgggtcact cgaggttgaa gtggcgctcg
      841 cgcatcgatt cgagtgattc cccgcgcagc gcctgaatcc ggatgacggc cagatcgaag
      901 aggattgcca tcgccagctc gaatgccgta cccatcgcca gcacgctagc gctgccccga
      961 tcgttggcca tggtctgggc cggaatcgtg agcgcgaaat cggccgattg cggatccggc
     1021 gcgtcgggct gggcggtgac cacgacggcc gacgccccgg cgcggtgcgc cagctcgatc
     1081 atgctgcggg tcaaggtcag gtcgcccggt cctgacgtga tgagcacgac atcgcccgtt
     1141 ccaacaggca tcgccgttac atcgcctgcc atgtgggcgt cgaatcccat gtgggcgagg
     1201 cgcatgcaaa agccgcgcag ggccagtccc tcgcgaccca ggccgaagca gacgacccgg
     1261 ttcgccccgg cgagcgtcgc gcagaacgtc tcgtaggcat ccgggtcgat tcggcccagg
     1321 acctcgccga gctcagcgat cgtcgagtcc cgaagctccg caaatgtcgc catctcgtta
     1381 cgctcctccg cgccctgccc ggactggcgg gttgcgagaa gttatgctgg ggtatgccca
     1441 gcccttacaa tagagcgata cacgcgcgcg tgcgccccgg ccgcgcgtta tcccgaggat
     1501 tatctcttgc cagacccgat tgaaactgaa gcacaagagt ccggcgcggc agcggttgag
     1561 gaaacgacct ccccggcgcc aaaacgacaa cgcaagccaa aagcccagaa gcggacccga
     1621 agcgagcgcc cgaacggctt gatgctgccg acgttgatcg tcgatgacat gatggtcctg
     1681 ccgaatctga ccgtcccgtt cccggttgag gacgaggaag cagcttacgt cttcgagcgg
     1741 gcgtgccgcg ccagcaagcg gctggtcttc ctcgtctgcg agcgaccgat ccagttgccc
     1801 gaacagcacg tcgatggggt agctgaccca tcgcggatca aggaattctt cgcctcgttc
     1861 gagcaggatg tggcggaagc ggaagttaac gggtgggtcg gcgagccgat gaacggcgat
     1921 tcgtccgatt acgagatttg cccggtcggg atcattgccg aggttggcca gttcggacca
     1981 ccgggccgcg ttccgcaggt catgctccag ggcatctcgc gcggcatcgt gctccaggtc
     2041 gcccaggaag agccgatgat ggtctgcgag gtcatcgctc acgaggaccc gatcccggcc
     2101 aagtcgctca gcgaagcgcc gatggccgcg ttgatcgagc agatcgagaa ctacgtcagc
     2161 atgcacccgg gcgcgcccga ggacgtcctg accatgctcg aggggatcga ggagcccggc
     2221 catctggccg acctggtcgc catctcgcct gagttcacgc cgctccagcg ccagcagatc
     2281 gttgagatgc tcgacccgct cgatcgcctt cgcgcggtca gcgtgatcgt cgagaagcgg
     2341 ctgatggtgc tgtcactgcg caacgcgatc gtcgccgacg cgcagtccgg tctcgacaag
     2401 cagcagcgcg agtatttcct gcgcgagcag attcgctcga tccagaagga gctgggcgaa
     2461 ggcacggccg aggagacgct tgccaaggac ctgcggcagc gcgtcgaaga ggcggggatg
     2521 cccgaggaga tcaaggccaa aacgctcgat caggtcgatc gtctcgagca acaacatccc
     2581 ttctcgcccg aaattggcgt catccgcagc tacgtcgagt ggctgatcga gctgccctgg
     2641 tcgactgaga gatcgg
//
LOCUS       DATAOZ010000647         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8931279, whole genome shotgun sequence.
ACCESSION   DATAOZ010000647 DATAOZ010000000
VERSION     DATAOZ010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8931279"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VHR64_16235"
     CDS             complement(<1..309)
                     /locus_tag="VHR64_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304436.1"
                     /translation="MTTDEQLLELFERSGALLTGHFLLSSGKHSDRYVEKFNLLRRPR
                     DTEAVCRGFVEKFQVAGIDVVAGPTTGGILLAFEVARQLGVDAAYAERASDGSLQREF
                     R"
     gene            complement(321..1373)
                     /gene="cax"
                     /locus_tag="VHR64_16240"
     CDS             complement(321..1373)
                     /gene="cax"
                     /locus_tag="VHR64_16240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX3304437.1"
                     /translation="MMKVMYGLLILVPIVIVLDVADIGSHSVIFTTSALAMIPLAALL
                     GQATEQVAFYTGEKVGGLLNATLGNAAELIITIIALREGLTEVVKASIAGSILGNILF
                     VLGASLLIGGVKNGEQSFDDKTAGVNASMLSLAVAVLIIPAVLALAPPESRPSHHDIE
                     RLSDGMAVIMIILYALYLLYTLRNGSSGTEIAHGGGPTMKLPIAIGILAGATIAIVIM
                     SEILVGALEPTAASWGLSEFFIGIMLVPLIGNIAEHLVAVQMAIQNKMDLSLGIAVGS
                     GLQIATFVTPVLVLAGWAMGTPMTLVFNRYELMGLLAAVIIAVLISVDGRSNWLEGAM
                     LLGLYAIVAFAFYYVP"
     gene            complement(1453..>1902)
                     /locus_tag="VHR64_16245"
     CDS             complement(1453..>1902)
                     /locus_tag="VHR64_16245"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX3304438.1"
                     /translation="KALISAGLAIKPKGSILISLADEDKADSLGVVQELVRLGYRIYA
                     TEGTAAWIQRAGMPVQLVTKRIGKGKPDVLDVILGQVVDGVINTPGPADKEVLDGLEI
                     RRAAVERGIPCVTSIDTARAMVSAMSQATSAYSVQTLADYRRVGRGY"
BASE COUNT          397 a          612 c          570 g          323 t
ORIGIN      
        1 ccggaactcg cgctggagcg agccatccga cgcgcgctct gcataggcag catcgactcc
       61 cagttggcgc gcgacctcga aagccagcag gatgccaccg gtcgtcggac cggctacgac
      121 atcgatcccg gcaacctgaa acttctcgac gaatccccgg cagacggctt cagtgtcccg
      181 tggtcgcctc aacaagttga acttctctac ataacgatcg ctgtgctttc cggacgagag
      241 caggaagtgc ccggtcagca gggcgccgct cctctcgaaa agctccagca attgctcgtc
      301 tgtggtcacg catgactcct ctagggaacg taatagaacg cgaaggcaac gatggcataa
      361 agcccaagca gcatcgcacc ctcgagccag ttcgaacggc catcaaccga gatcaggacg
      421 gcgatgatga ccgccgcaag caagcccatc aactcatagc gattgaagac aagggtcatc
      481 ggtgtgccca tcgcccagcc ggccaggacg aggaccggcg ttacgaaggt tgcgatctgc
      541 aggccggacc caaccgcaat gccaaggctg agatccatct tgttctggat ggccatctgc
      601 acggcgacca gatgctccgc gatattgccg atcaacggaa cgagcatgat gccgatgaag
      661 aactcgctca gaccccacga tgccgcggtc ggctcaagcg caccgaccag gatctccgac
      721 atgatcacga ttgcgatcgt tgcgccggcc aggattccga tcgcgatggg gagcttcatc
      781 gtcggcccac cgccgtgcgc gatctcggtc ccgctcgagc cattccgcag cgtgtagagc
      841 aggtacagcg catagaggat gatcatgatg acggccatac cgtcgcttaa tcgctcgatg
      901 tcgtggtgcg aaggacgtga ttccggcggc gccagggcga gtacggccgg gatgatcagg
      961 acggcaacgg ccagagacag catcgacgca ttcacgccgg cggtcttatc gtcaaagctc
     1021 tgctcgccat tcttcacacc gccgatcagc agcgaagcgc cgaggacgaa gaggatattg
     1081 ccgaggatcg acccggcgat gctggccttg acgacctcgg tcaagccctc gcgaagggcg
     1141 atgatcgtga tgatcagctc ggcggcgttc ccgagcgtgg cgttgagcaa accaccgact
     1201 ttctcacccg tgtagaaggc gacctgctcg gttgcctgac ccaggagggc ggccagcggg
     1261 atcattgcca acgccgaagt ggtgaagatg acggagtggc ttccgatgtc cgcaacgtcg
     1321 agcacgatta cgatcggcac gaggatcagg agcccgtaca tcaccttcat catgcggcga
     1381 aacccctccc caaacgtcgc acagccattg aattatgcga gtagctcagc gcaagcccgt
     1441 gagggccgcg acctaatatc cgcgcccgac ccggcggtaa tccgccagcg tctgcacgct
     1501 gtacgccgac gttgcctgac tcatggccga gaccatggcc cgcgcggtgt cgatcgacgt
     1561 cacgcacggg atgccgcgct caactgccgc tcggcggatc tcgaggccat ccaggacctc
     1621 cttgtcggcc ggtccgggcg tgttgatgac gccatcgacc acctggccca ggatgacgtc
     1681 gagtacgtcc ggcttgccct tgccgatgcg cttcgtgaca agctggacgg gcatcccggc
     1741 gcgctggatc caggcggcgg tgccttcggt tgcataaatc ctgtagccca ggcgaaccag
     1801 ctcctggacg acaccgagcg aatcggcctt gtcctcgtca gcgagcgaga tcaggatcga
     1861 gcccttcggc ttgatcgcga gtccagccga aatcagcgcc tt
//
LOCUS       DATAOZ010000648         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1563505, whole genome shotgun sequence.
ACCESSION   DATAOZ010000648 DATAOZ010000000
VERSION     DATAOZ010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563505"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VHR64_16250"
     CDS             complement(<1..455)
                     /locus_tag="VHR64_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3304439.1"
                     /translation="MHPDLEQAISFHEAIVAEGGKALVGYDTAKALANVVLMSEIPTS
                     YDKKEQRQVNAGNLLLRGVPGVGKTFFGVILAAISNAKFVRLQGRADLQPTEVVGFQM
                     INPATGELMTEFGPMAQAEVILLDEINRIPLKSQSAFLEALQDRTVTVG"
     gene            496..1188
                     /locus_tag="VHR64_16255"
     CDS             496..1188
                     /locus_tag="VHR64_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX3304440.1"
                     /translation="MNKVLATAADAVATIAEGATIMMGGFGLCGIPENLIAALHARGT
                     RGLTLISNNAGVDDFGIGLLLRAGQVRKMISTYVGENKEFERQYLAGELDVELVPQGT
                     FAERIRAGGAGVGGFFTPTGYGTLAAEGKETRLIDGKPFVLERPLTADFAFVKAWRGD
                     TAGNLVYRRTARNFNPVMATAAAVTIAEVEHLVEPGEIDPDHVITPGIFVRYVLQGEK
                     YEKRIEKRTVRP"
     gene            1268..>1831
                     /locus_tag="VHR64_16260"
     CDS             1268..>1831
                     /locus_tag="VHR64_16260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX3304441.1"
                     /translation="MDVRDRIVLRGARELKDGAYVNLGIGMPTLVANHIPAGVHITLH
                     SENGLLGIGPYPTEAEVDPDLINAGKETVTETPGCSYFNSADSFAMVRGGHIDLTVLG
                     ALQVDEHGNLANWMIPGKMVKGMGGAMDLVASARRVVVAMEHTTKDGKPKILKKCTLP
                     ITGLKVVDTIVTEMAYMRVTPQGLVLEE"
BASE COUNT          352 a          579 c          584 g          316 t
ORIGIN      
        1 ttgccgaccg tcaccgtccg atcctgcagc gcctcgagga atgcgctctg cgacttcagc
       61 gggatgcggt tgatctcgtc gagcaggatg acctcggcct gcgccatcgg tccgaactcg
      121 gtcatcagct cgccggtcgc cggattgatc atctggaagc cgacgacttc ggtcggctgc
      181 agatcggcgc gaccctgcag gcggacgaac ttcgcgttgc tgatcgcggc gaggatgacg
      241 ccgaagaacg tcttgcccac cccggggacg ccccgcagca gcaggttgcc ggcgttgacc
      301 tggcgctgtt ctttcttgtc gtacgaagtc ggaatctccg acatcaggac gacgttggcg
      361 agcgctttcg cggtgtcgta cccgacgagc gcttttccgc cttcagcgac gatcgcttcg
      421 tggaacgaga tggcttgttc gaggtcagga tgcatcgaat tgggacagac atcttacttg
      481 atagaatgga attcgatgaa caaggtgctc gccacggccg ccgacgccgt cgcgacgatc
      541 gccgaaggcg ccacgatcat gatgggcggc ttcggactgt gcggcatccc cgagaatctc
      601 atcgccgcgc tgcacgcgcg cggaacccgc gggctcaccc tgatcagcaa caacgccggc
      661 gtcgacgatt tcgggatcgg cctgctgctc cgggccggcc aggtccgcaa gatgatctcg
      721 acctatgtcg gcgagaacaa ggagttcgag cgccagtacc tcgccggcga gctcgacgtc
      781 gagctggtgc cgcagggcac gtttgccgaa cggatccgcg ccggcggcgc cggggttggc
      841 ggtttcttta cgccgaccgg atatgggacg ctcgccgcag agggcaagga aacgcgcctc
      901 atcgacggca agccgttcgt cctcgagcgg cccctcactg ccgatttcgc gttcgtcaag
      961 gcctggcggg gcgacaccgc cggcaacctg gtctatcgcc gaaccgcgcg caacttcaac
     1021 ccggtgatgg cgaccgccgc cgccgtgacc attgccgagg tcgagcacct ggtcgagccc
     1081 ggcgaaatcg atcccgatca cgtgatcacg ccggggatct tcgtgcgcta cgttctgcaa
     1141 ggcgagaaat acgagaagcg gatcgagaaa cgcacggtgc ggccatgact tgcgagagca
     1201 gcaatgagta tcacggaatt cgagatcgtc gcgtgtcttc gtgccttcgt ggtgcaccgt
     1261 gaagccgatg gatgtccgtg accggatcgt cttgcgaggg gcgcgcgagc tgaaggacgg
     1321 cgcctacgtg aacctcggca tcggcatgcc gacgctcgtc gccaatcaca ttccggccgg
     1381 cgtgcacatc acgctccatt ccgagaacgg cctgctcggc atcggtccgt atccgaccga
     1441 ggccgaagtc gatcccgatt tgatcaacgc cggcaaggaa acggtcaccg aaacgcccgg
     1501 ctgctcctac ttcaacagcg ccgactcgtt cgcgatggtg cgcggcggcc acatcgacct
     1561 caccgtcctg ggagcactgc aggtggacga gcacggcaac ctcgcgaact ggatgattcc
     1621 gggcaaaatg gtgaaaggga tgggcggcgc catggatctc gtcgcaagtg cccgccgcgt
     1681 ggtcgtggcg atggagcaca caacaaaaga cggaaaaccc aagatcctga aaaaatgcac
     1741 gctacccatc accgggctca aggtcgtaga cacgatcgtg accgagatgg cttacatgcg
     1801 cgtcaccccg cagggcctgg tgctggaaga a
//
LOCUS       DATAOZ010000649         6683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2091422, whole genome shotgun sequence.
ACCESSION   DATAOZ010000649 DATAOZ010000000
VERSION     DATAOZ010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2091422"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1854)
                     /locus_tag="VHR64_16265"
     CDS             complement(<1..1854)
                     /locus_tag="VHR64_16265"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX3304442.1"
                     /translation="MTKQAAPVSMEAPRTHPAPQMRVASQFGWIGVHPPLSKYYSPYA
                     TRNKPRRWPAVLAVLLALLVGAGVGIAATGGVDWFSSSDKNPTETPRAVAANDETSTP
                     GQQDEAAPTATDEPQDEQPAATNTAVPEQSDPTETPKPTATTTPENTPTPDAGPPDEA
                     AKRFLTTWSTGDYHALYKMISAASKATISEQDFVDRFDAINTEAGITEVKVESTSAPD
                     LDLNMPIKLSYKTGTVGDFSQTNTIHLVREGPEWHVDWSPSLIFTQLGDGCVDFMVQT
                     VRRGSILDRNGNPLAYDGTASVIGVIPGQFQNEDAELKALSKIVGIDTKDIKSMYADA
                     DPGWFVPIKSYANQVDDATKTKLSAIPGAALRSQTMRIYPLGKQAAHITGYVTRVTAE
                     DIDSDPTGELAGIDWIGRAGIEAGANDLLTGRPGGRLMVVDCNTRAERTEIASRRPQE
                     PKDIVLTVDKDFQVQVDNALGDVKGSAIVIDPRTGGILALASHPAYDPNWFVSGMTGK
                     DRDFINNEDERPLLDRAAEAAYPTGSIFKVITMAAAMHDLKYTGDTQIDCPQEFSLPG
                     TDQVWRDWTYEEGLGAQGMLTLHTGLVSSCNTVFYQIGAALDEQNEEWLPDM"
     gene            1983..3194
                     /locus_tag="VHR64_16270"
     CDS             1983..3194
                     /locus_tag="VHR64_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX3304443.1"
                     /translation="MTSPQSDHRPLAGIRVVDLTRVMTGPYCTMMLGDLGADVIKVEL
                     PGKGDDTRAWGPPFINGEAAYYLSVNRNKRSIALNLKSDAGKAALWKLIESADVVVEN
                     FSPGTIARLGFGWEVIHARNPKAIMASISGFGQTGPGSQRTAYDLVVQGMGGIMSVTG
                     QVGGPPTKYGIPIGDIGAGMFAAYAIAAALFRRSVTGEGQYIDVSMLGGQIALLTYQA
                     GAYFATGVAPKAAGNAHSIIVPYDAFPTADGYVIIAVGNDSLFQRFCGAAGLDETGRD
                     PRFATNALRVENKSALYEAINGALGKLTTDEVIERLDKVAVPCGPIQTIDRVFENEQV
                     LHSGLKRTVQHPTAGEITLTGFPYHFSEGELCVEEAPPLLGQQTREILAEAGYSEHEI
                     DDMINSGAAAE"
     gene            complement(3456..4067)
                     /locus_tag="VHR64_16275"
     CDS             complement(3456..4067)
                     /locus_tag="VHR64_16275"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEX3304444.1"
                     /translation="MQQTADVRHDGPVRRADSASTRMIIALDRVIYRFAQRWLFGFNL
                     VVGAYALAVILAPILRALGYGGAAKPIYGFFGLFCHQDPARSFHLFGQQFACCERCAA
                     IYGSIALFGLLFAFGRDQIRRPTPVAMVLLVSPVVLDGMAVGSGLYGGNAVMRVITGA
                     LFGLAMIWFLYPRFETGFRVMRNRIETLFDRLVAQGRAKPLSA"
     gene            4352..4969
                     /locus_tag="VHR64_16280"
     CDS             4352..4969
                     /locus_tag="VHR64_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304445.1"
                     /translation="MDRNTQTHSRRHVLKLVAGAALAAVPALRVVEEAAAARGWCRLD
                     PTFLVDGFVGNVYVSGELDTTYDTTGPIQMLFRVPAGCQVDLISTDPGFGHGYDINYD
                     YSPDLKTDHKKIDIEIQVYVPSVTDKLPIRVEFVPNTTVEVEDRKDGNTNKWIKVKTR
                     LRKPEETKEQKEAREADEKAAKEAEKAAKKAAKDAEKADDSAGAP"
     gene            5257..5685
                     /locus_tag="VHR64_16285"
     CDS             5257..5685
                     /locus_tag="VHR64_16285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX3304446.1"
                     /translation="MAFEIDTSTTFGAAVIDRLEAQKLAWLTTVDSTGTPQPNPVWFI
                     WDDGMIYIFSKPNQAKLNNIARSGRVAVNLEATADEEHVTIFTGQAEIIDRADLPADI
                     LDRYAERYKEGMKGIQMTRAEYEAAYTIPIRFTPEKLRGW"
     gene            6013..>6683
                     /locus_tag="VHR64_16290"
     CDS             6013..>6683
                     /locus_tag="VHR64_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3304447.1"
                     /translation="MAPSADAVGFSDLLPRPVTSLVGRAADVGRIRARLLEDDCRLLT
                     LLGPGGVGKTRLAIAVADQIGTADGIPFIDLAHVRDGSAVLPAIAAHLAVTDSDRAPL
                     DAVAERLRDGYTVLVLDNLEQIVGVAAPVARLIEACPGLTILATSRTPVGLHGEQRWP
                     VAPLPVPTSSNLTDIECSPAVDLFVRRARLARPDLVLTKEIAADVAAICAHLDGLPLA
                     LELAAA"
BASE COUNT         1193 a         2153 c         2143 g         1194 t
ORIGIN      
        1 catgtcgggc agccactcct cgttctgctc atcgagggcg gcgccgatct gatagaagac
       61 ggtgttgcag gagctgacca aaccggtgtg gagcgtcagc atgccctgcg cgcccagccc
      121 ttcctcatac gtccagtccc gccagacctg atcagtccct ggcaggctga actcctgcgg
      181 gcagtcaatc tgggtgtcgc cggtgtactt gaggtcgtgc atggccgcgg ccatcgtgat
      241 gaccttgaag atcgagccgg tcgggtatgc cgcctccgcc gcgcggtcca gcagcggtcg
      301 ctcgtcctcg ttattgatga agtcgcggtc cttaccggtc attccgctca cgaaccagtt
      361 cgggtcgtag gccggatggc tggccagcgc gaggattccg ccggtgcgcg ggtcgatcac
      421 gatcgcactg cccttgacat cgccgagcgc gttgtcgacc tggacctgga agtccttgtc
      481 gacggtcagg acgatgtcct tcggctcctg cgggcgacgc gaggcgatct cggttcgctc
      541 ggcgcgggtg ttgcaatcga ccaccatcaa ccgtccgccg ggccgcccgg tcagtagatc
      601 gtttgcgccg gcttcgatcc cggcccggcc gatccagtcg atcccggcca gctcgccggt
      661 cggatcgctg tcgatgtcct cggcggtgac acgagtgaca tagccggtga tatgcgcggc
      721 ctgcttgccg agcgggtaga tccgcatggt ctgcgagcgc agggccgcgc cggggatcgc
      781 cgagagcttg gtcttggtcg catcgtcgac ctggttcgca tagctcttga tcggcacgaa
      841 ccagcccgga tcggcatccg catacatcga cttgatgtct ttggtatcga tcccgacgat
      901 cttgctgagc gccttcagct cggcgtcctc gttctggaac tggccgggga tgacgccgat
      961 cacgctggcc gtaccgtcgt acgccagcgg gttgccgttg cggtcaagga tgctgccgcg
     1021 ccggacggtc tggaccatga aatcgacaca gccatcgccg agttgggtga agatcagcga
     1081 cggcgaccag tcgacgtgcc actccggacc ctcgcggacg aggtggatcg tgttggtctg
     1141 ggagaagtcg ccgacggtgc cggtcttgta cgagagtttg atcggcatgt tgaggtcgag
     1201 gtcgggcgcg ctggtcgact cgaccttgac ctcggtgatg ccggcctcgg tgttgatcgc
     1261 gtcgaaacgg tcgacgaagt cctgttcgct gatggtcgcc tttgaggccg cgctgatcat
     1321 cttgtagagc gcgtggtagt cgccggtgct ccaggtcgtc aggaagcgct tggccgcctc
     1381 atccggcgga ccggcatcgg gcgtgggcgt gttctcgggc gtggtggtcg cggtcggctt
     1441 gggtgtctcg gttgggtcgg actgttccgg gacggcagtg ttggttgcgg ccggctgctc
     1501 gtcctgcggc tcgtcggtgg cggtcggcgc ggcctcgtcc tgctgcccgg gcgtgctggt
     1561 ctcgtcgttg gcggcgacgg cgcggggtgt ctcggtcggg ttcttgtcgc tgctcgagaa
     1621 ccagtccacg ccacccgtcg ccgcaatgcc gacgcccgca cccacaagaa gcgcgaggag
     1681 aacggcgagg acggctggcc accgtcgggg tttgttcctg gtggcgtacg gagagtagta
     1741 cttcgacaac ggcggatgaa ctccgatcca accaaactgg ctggccacac gcatttgcgg
     1801 agccgggtgc gtgcggggcg cttccatgct gacaggggca gcctgtttcg tcaagcaatg
     1861 caccctgctc tgcataacgt aacggatagc gggcgaatcg ggtttccttg ccggtcgcgt
     1921 gccctccact tgctacaatt cgagctcgca atggccgtat ctcgcgtgca gaaggatccc
     1981 tcgtgaccag ccctcaatcc gatcatcgtc cgcttgccgg tatcagggtc gtcgacctga
     2041 cccgcgtcat gaccggcccc tactgcacga tgatgctcgg cgatcttggc gcggacgtga
     2101 tcaaggtcga gctgcccggc aaaggtgacg atacccgtgc ctgggggcct ccctttatta
     2161 atggagaggc ggcctactac ctttccgtaa atcgcaacaa gcgctcgatc gccctcaacc
     2221 tcaagagcga cgccggcaag gcagcgctct ggaagctgat cgagagcgcg gatgtcgtcg
     2281 tcgagaactt ctcgcccggc acgatcgccc ggctcggatt cggctgggag gtgatccacg
     2341 cgcgcaaccc gaaggcgatc atggcctcca tctccggctt cggccagacc ggccctggca
     2401 gccagcgcac cgcctatgac ctcgtcgtgc agggcatggg cgggatcatg agcgtgaccg
     2461 gacaggtcgg tgggccgccg accaagtacg ggatcccgat cggcgacatc ggcgccggca
     2521 tgttcgcggc ctatgcgatc gccgccgcac tcttccggcg gagcgtgacg ggcgagggcc
     2581 agtacatcga tgtctcaatg ctcggcggtc agatcgcgct gctgacctac caggctggcg
     2641 cctacttcgc gaccggtgtc gcaccgaagg ctgccggcaa tgcccactcg atcatcgtcc
     2701 cgtacgacgc cttcccgacc gccgatggct acgtgatcat cgcggtcggc aacgacagcc
     2761 tgttccagcg cttctgcgga gcggccggcc tcgacgagac cggccgggat ccgcgcttcg
     2821 ccaccaacgc gctccgtgtc gagaacaagt cggctctcta tgaagcgatc aacggtgcgc
     2881 tcggcaagct gacgaccgac gaggtgatcg agcggttgga taaagtggca gtgccgtgcg
     2941 ggccgatcca gacgatcgat cgcgtcttcg agaatgagca ggtgctgcac agcggcctga
     3001 agcggaccgt ccagcatccg accgccgggg agattacgct taccggattc ccgtaccact
     3061 tctcggaagg cgagctgtgc gttgaggaag ctccgcccct cctcggccaa caaacccgcg
     3121 agatcctggc cgaagccggc tactccgaac atgagatcga cgacatgatc aactcaggcg
     3181 ccgccgccga ataagcctcc ccgctccaac tttgtcgaca tcctgttccc gtgcctgaag
     3241 acgcgcactg aaatctgatc ccgactgaag ccggctggat gatcgccgtg gcttgtaggg
     3301 gcccgcggca gcgggccagc gccttcccgc caagcctgcc acgtgccagg aaacccgacg
     3361 atcacccggc cgtgtaacac gccagcagcc aatagaaggc gctcgggttt tcgacagcaa
     3421 acccctacat tcgtccatgc atgttgcggt gatgttcagg cagaaagcgg tttggcgcgg
     3481 ccctgggcga cgaggcggtc gaagagggtc tcgatccggt tgcgcatcac cctgaagccg
     3541 gtctcgaagc gcgggtaaag gaaccagatc atggccagcc cgaatagcgc gcccgtaatc
     3601 actcgcatca ccgcgttgcc gccgtacaac cccgagccga ccgccatgcc atcaagcacg
     3661 accggagaga ccagcagcac catggcgaca ggcgtcgggc ggcggatttg gtcgcggccg
     3721 aacgcgaaga gcaggccgaa gagcgcgatc gatccgtaga tcgccgcgca gcgctcgcag
     3781 caggcgaatt gctggccgaa gaggtggaag ctgcgagcgg gatcctggtg gcagaagagt
     3841 ccgaagaagc cgtagatcgg tttcgccgcg ccgccatagc cgagcgcgcg caggatcggg
     3901 gccagaatca ccgccagcgc atacgcgccg accaccaggt tgaacccgaa cagccagcgc
     3961 tgcgcgaacc ggtagatcac ccggtcgagc gcgatgatca tcctggttga ggcagagtcg
     4021 gcgcgccgga ccggaccatc atgtcgaacg tctgccgttt gctgcatggg aaaggttgcc
     4081 tcgtgcgagc ggggtactgc gctcagggta ggccatcgac caatcgcccg ggtatggccc
     4141 atcggtcgcg actttcgcct gtaaggcagg cgcctcgtcg acgcgagtag gcaaatccct
     4201 gtctgatgta gtccgaacgt cccgactgag cggacaagtg gcatggctgt ttacccacct
     4261 gccggtcatg ggccgaccgg ggtaaccatc ctaggatgat ggtcatgtcg acgccggctg
     4321 cccccgtcgc atcacgttgg gaggattctc gatggatcga aacacgcaga cccacagtcg
     4381 gcgtcacgtg ctcaagctgg ttgccggagc ggcgctcgcg gcagtgccgg cgctccgggt
     4441 ggtggaggag gctgccgcgg ctcgcggttg gtgccggctc gacccgactt tcctcgtcga
     4501 tggcttcgtc ggcaatgtct acgtctccgg tgagctcgac acgacctacg acaccacggg
     4561 cccaatccag atgctcttcc gggtccctgc gggctgccag gtagacctca tttcaacgga
     4621 tcccggcttc ggccacggct atgacatcaa ctacgactac tctccggatc tgaagaccga
     4681 tcacaagaag atcgacatcg agatccaggt ctacgtgccc tcggtcaccg acaagttgcc
     4741 gatccgcgtc gagttcgtgc ccaacacgac ggtcgaggtc gaggatcgca aggacggcaa
     4801 caccaacaag tggatcaagg tgaagacccg gctccgcaag cctgaagaga ccaaagagca
     4861 gaaagaagcc cgcgaagccg atgagaaggc ggccaaggaa gcggaaaaag ctgccaaaaa
     4921 ggccgccaag gacgcggaga aggcggatga ttccgccggg gcgccgtaga tcggatccga
     4981 actattcccg ctgcccgcac ataccgatct gacacaggag catggcaatg acgccacgct
     5041 cctgtgtcgt ttctcgatga tgtttcaatg aatgccacgt ggctgatccg ggctcagcgc
     5101 cgaattcatc agccgattcg cgtcgctccg tgattgacga catgggattg caatcggtag
     5161 gttcgattgt cgagatggta ttgcgctgct tgccgaacgt gccgcgaacc cggatgatgt
     5221 gcccactgag cgacgacaga atccaaggag gcgcatgtgg cattcgagat cgatacgtcg
     5281 acgacgtttg gggctgcggt gatcgaccgg ctcgaggcgc agaagctggc ctggctgacg
     5341 accgtggaca gcacgggcac gccgcagcca aacccggtct ggttcatctg ggatgacggc
     5401 atgatctata tcttcagcaa gccgaaccag gccaagctga acaacatcgc ccgctccggc
     5461 cgcgtcgcgg tcaacctcga ggcgaccgcc gacgaggagc acgtcacgat cttcaccggc
     5521 caggccgaga tcatcgatcg cgccgatctg cctgcggaca tcctcgatcg ctacgccgag
     5581 cggtacaagg agggcatgaa ggggatccag atgacccggg ccgagtacga ggcggcctac
     5641 accatcccga tccgcttcac cccggagaag ctccgtggct ggtagggaca atcctgacat
     5701 acggcgccct gagccctcgc cctggacccc gcagagagtt caaattagcc ggccacactg
     5761 tccgctgctc gttatggtgt cggcggcgca ccgccatcga cgggacgatc gagcgatagg
     5821 tgtcttgcca gtacctgaat cgcaattctg tttgccccgg cgaacctaaa tggacacctc
     5881 gtccagccgc tcccatgaag cgcattccgg gttgctccgt gttgtgacag cacgaatttg
     5941 ccaccactac aatgctgtca cgcatctgat cagcggacgc atgatcgaaa ggcacactga
     6001 gtggcggaac gagtggctcc atcagcggac gccgtcggct tctctgacct gttacctcgt
     6061 cccgtcacat cgctggtcgg ccgcgcggcc gacgtcggac ggattcgcgc ccggttgctc
     6121 gaggacgatt gccgcctgct gaccttgctc ggaccaggtg gcgtcggcaa aacccgcctc
     6181 gcgatcgcgg ttgcggacca gatcggcacc gcggacggca tcccgttcat cgacctggcc
     6241 catgtgcgag acggatcagc cgtcttgccg gcaattgccg cgcacctcgc ggttacggac
     6301 agcgatcggg ccccgctgga cgcggtcgcc gaacggctcc gcgacgggta caccgtgctg
     6361 gtcctggaca atctcgagca gatcgttggg gtggccgctc cggtcgcccg cctcatcgaa
     6421 gcttgtccgg gcttgaccat cctggctacg agtcgcacgc cggtgggtct gcacggcgaa
     6481 caacgctggc cggtcgcccc gctccccgtg ccaacctcat ccaacctcac cgacatcgaa
     6541 tgcagcccgg cggtcgatct gttcgtccgg cgtgcccgcc tggcgcgacc tgaccttgtg
     6601 ctcaccaaag agatcgcggc cgatgtcgcc gcgatttgcg cccacctcga cgggctcccc
     6661 ctggccctgg aactggccgc cgc
//
LOCUS       DATAOZ010000650         3431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9195565, whole genome shotgun sequence.
ACCESSION   DATAOZ010000650 DATAOZ010000000
VERSION     DATAOZ010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9195565"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VHR64_16295"
     CDS             complement(<1..947)
                     /locus_tag="VHR64_16295"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX3304448.1"
                     /translation="MTGTQDLTAAVPQVPQSRLRASLHVVRWVIGEALLQGAVLIVLA
                     WLIPGFVIGGPASVVLAAVGFVLVQAAAWPFIYQLSARLHPLVFPIMSLALNALLLVL
                     FTDLISDLEIGHVHVSSPWIAGLIVVALTVGSTLLGALFSLRDDDAYDWFVTRPLRRT
                     YSDAETSDVPGILFLEIDGLSEPILRQAIHGGWMPTVARWVSSGSHTITGWEPDLSSQ
                     TSSSQAGILLGDNTDIPAYRWFDKGEGRLMVSSSMSTARVLEEQLSRGIGLLRGGGSR
                     WNVFSGDAADSVCTYSTFGDRSRPGSGSYLAYFAKGIDRP"
     gene            complement(949..2364)
                     /locus_tag="VHR64_16300"
     CDS             complement(949..2364)
                     /locus_tag="VHR64_16300"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3304449.1"
                     /translation="MRRMRRGRHKARAKEWRPGYRDVLRNPAVEVVLFSHVLSKAGSQ
                     ILGYGSMVHLARIGGSQIEISLLSASGSLAALAFGVRGGAVADSMPKRVALGLSYSAQ
                     AALCFVVPRYLGTGVGALLLLIFAVSILTQITSPALKVAVAVVATAAELANVAVLLGF
                     AGGIGTAIGAAVLAPVLIKQWGITIAMYAAGALFLFASIRAVRIPEDPKRAARAEAER
                     PRIGGFREAGAWMLAHQAVSSMILVGAAVTVLSRIFDSLQPIYVRSVLGADPANAIYI
                     FAPGAIGALIGQLASPILIRWPGERWLAVIALAVFSGAMVLFGLITVVADTLARFSPL
                     RVLSLLDIHLSDQILAAGMIAILVEFAQAGAATSVQTYVNRRVPLDVQGTTFGVQSFL
                     TNGMGFAGTLLVGALATVFGTRAIFLLVPPMLAAALIWVIRTAYRRRDEPVPTRREAL
                     AALWSEPPDPDASDASAGPVR"
     gene            2618..>3431
                     /locus_tag="VHR64_16305"
     CDS             2618..>3431
                     /locus_tag="VHR64_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304450.1"
                     /translation="MNQRASAIATIDPSRFQRAGSVDDRFQSYNVEMVEVIGGRFWKP
                     YAAKSDDQSPAAQSSGVPVGMDPDLYQYRSPIDLGNPRLRTLASALGPAYVRVSGTWA
                     NSVYFHDSDTPPPDRPPDQFGGMLTRQQWQGVIEFADAVDARIVTSVATGAGVRDADG
                     VWSPDQAVALLNYTRALGGMIAATEFMNEPTFALMGGAPAGYDAAAFGRDISVFRSFL
                     KQTSPETIFLGPGSVGEGGTAFELGPEMLRTEQLLEATGPAFDAFSYHFYGGA"
BASE COUNT          639 a         1127 c         1162 g          503 t
ORIGIN      
        1 ggacggtcga tccccttggc gaagtaggcg agatagctgc cggagccggg tcgggagcga
       61 tcgccgaagg tgctgtaggt gcagacgctg tcggccgcgt cgcccgagaa gacgttccag
      121 cgactcccgc cgccgcgcag cagtccgatc ccgcgcgaga gctgctcctc gaggacgcgg
      181 gcagtgctca tgctgctcga gaccattagc ctgccctcgc ccttgtcgaa ccagcggtag
      241 gcggggatgt cggtgttgtc gccgaggagg atcccggcct ggctggacga ggtctgcgag
      301 gagagatccg gttcccagcc ggtgatcgtg tgcgatccgc tcgataccca gcgggcgacg
      361 gtcggcatcc agccaccgtg aatcgcctgg cgcagaatcg gctcggacag cccgtcgatc
      421 tcgaggaaga ggatgcccgg cacgtcggac gtttccgcat cggagtacgt gcgacgcagc
      481 gggcgagtta cgaaccaatc gtaggcatcg tcgtctcgca gggagaagag tgcgcccaga
      541 agcgtgctgc ccacggtcag ggcgacgacg atgaggccgg cgatccaggg actggacaca
      601 tggacgtgac cgatttcgag atcgctgatc aggtcggtga acagcacgag gagaagggcg
      661 ttcagcgcga ggctcatgat cgggaagacc agcgggtgca ggcgtgctga gagctggtag
      721 atgaaaggcc aggccgcggc ctggacaagc acgaatccga cggcggcaag gaccaccgag
      781 gccggcccgc cgatgacgaa cccgggaatc aaccaggcta gcacgatcag caccgcgccc
      841 tggagcagcg cctcgccgat cacccaccgc acgacatgga ggctggcgcg caaccgggat
      901 tgcggcactt gcgggacggc tgcggtcaag tcctgtgtgc cagtcacctc aacgcaccgg
      961 cccggcggac gcatcggacg catccgggtc cggcggttcg ctccagagcg cggccaacgc
     1021 ctcccgccgg gtcgggaccg gctcatcccg ccgccggtag gccgtgcgaa tcacccagat
     1081 cagcgcggcg gccagcatcg gcggcacgag caggaagatc gcgcgcgtac cgaacaccgt
     1141 cgccaatgct ccgaccaata gcgtgccggc aaaacccatt ccattggtca ggaacgactg
     1201 caccccgaaa gtggtgccct ggacgtcgag cgggacacgg cggttgacat aggtctggac
     1261 cgaggttgcc gcgccggcct gggcgaactc gacgaggatc gcgatcatgc cggccgccag
     1321 gatctggtca ctcagatgga tgtcgagcag cgacagcacc cgcagcgggc tgaatcgggc
     1381 cagcgtgtcc gcgacaacgg tgatcaggcc gaagaggacc atcgcgccgc tgaacacggc
     1441 cagcgcgata acggccagcc agcgctctcc cggccatcgg atcaggatcg gggacgcgag
     1501 ctgcccgatc agcgcgccga tcgctccggg cgcgaagatg tagatcgcat tggccggatc
     1561 ggccccgagc acggagcgca catagatcgg ctgcagggaa tcaaagatac gcgagaggac
     1621 ggtgaccgcc gcgccgacca ggatcatcga ggagaccgcc tggtgcgcga gcatccatgc
     1681 gccggcctcg cgaaatccgc cgatccgcgg acgctcagcc tcagcccggg cggctcgttt
     1741 ggggtcttcg gggatacgga ccgcgcggat cgaggcgaag aggaagagcg cgccggccgc
     1801 atacatcgcg atcgtgatgc cccactgctt gatcagcacc ggcgccagga cggcggctcc
     1861 gatcgcagta ccgatccccc cggcgaagcc gagcagcacc gccacattcg ccagctccgc
     1921 ggccgtggcg acgacggcga cggcgacctt gagcgcgggc gacgtgatct gggtcaggat
     1981 gctgaccgcg aagatgagca gaagaagcgc cccgaccccg gttccgaggt agcggggcac
     2041 gacgaagcaa agcgccgcct gcgccgaata gctcaggccg agcgcgaccc gcttcggcat
     2101 cgaatcggcg acggccccgc ctcgcacgcc gaaggcgagc gcggccaggg agccactcgc
     2161 gctcagcagg gagatctcga tctgcgagcc accgatccgg gcaaggtgga ccatcgagcc
     2221 gtagcccagg atctgcgacc ctgccttgga cagaacgtgc gagaagagca cgacctcgac
     2281 cgccgggttc cgcaggacat cgcggtatcc cggccgccac tcctttgccc gcgccttgtg
     2341 ccgaccgcgt ctcatgcgtc gcattgtgcg tcattggtca tatcggagcc gagtcaggtc
     2401 agaggaggtt gcgcccgctc ctgcagcgca cgcttaatgt cgccaagaat caggaatcac
     2461 ggggaaaccg gcctatcctc gggctacgct gagctgttga cgcacatctc cgaaaggcgc
     2521 ccactgtgac cgacctgacc gccaacatct ccctggcgcg atggccccgc cggcggacaa
     2581 tcaacgtgac agctctgccc agaactgagc gcgggatatg aaccagcgtg cctcagcgat
     2641 tgccaccatc gatccatcgc ggtttcagcg tgccggctca gtggacgacc gcttccagtc
     2701 ctacaacgtg gagatggtcg aggtcatcgg aggccggttc tggaagccct acgccgcgaa
     2761 atccgatgac cagtcaccgg cagcgcagtc aagcggggtt ccggtcggca tggacccgga
     2821 cctctatcaa taccggtcgc cgatcgatct cggcaacccg cggctacgga cgctggcttc
     2881 cgcacttggt cctgcttacg tgcgggtcag cggaacctgg gcgaacagcg tctatttcca
     2941 tgactcggac acgcctccgc cggaccggcc gccggatcag ttcggcggca tgctgacccg
     3001 ccagcaatgg cagggggtca tcgagtttgc cgacgcagtc gacgcgcgga tcgtcacgtc
     3061 ggtcgcgacc ggagccggag tccgggatgc tgacggcgta tggtcgccgg atcaggctgt
     3121 ggcgttgctc aactacaccc gggcgctcgg gggcatgatc gccgcgactg agttcatgaa
     3181 cgagccgacc ttcgccttga tgggcggcgc gccggctggg tacgacgcgg cagccttcgg
     3241 gcgggatatt tccgtgttcc gctcgttcct caagcagacc tcgccggaaa cgatcttcct
     3301 tggcccgggc tctgtcggag agggaggaac tgccttcgag ctgggtccgg agatgcttcg
     3361 gaccgaacag ctcctcgaag cgaccgggcc ggcgttcgat gccttctcgt atcacttcta
     3421 tggtggcgcc t
//
LOCUS       DATAOZ010000651         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8670862, whole genome shotgun sequence.
ACCESSION   DATAOZ010000651 DATAOZ010000000
VERSION     DATAOZ010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8670862"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VHR64_16310"
     CDS             <1..466
                     /locus_tag="VHR64_16310"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX3304451.1"
                     /translation="NLASLIKEVRRALDDTAETPRFIRTVHRFGYWFIGELREDSASQ
                     TPTPQSVRYWLVWLSRQIPVQAGEHILGRAPDASVWIDAAGVSRQHARLVVDDHGATV
                     EDLGSKNGTFVGDQPVTAARPLLDGDQIRLGPVVITFRIPPPVGATDTVQSD"
     gene            complement(479..3301)
                     /locus_tag="VHR64_16315"
     CDS             complement(479..3301)
                     /locus_tag="VHR64_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX3304452.1"
                     /translation="MNVDAAPRPPAGPYRILDLIGQGGGGEVYRAWDPRLEREVALKI
                     LHRRLAIDAGRLDRFVAEARAAGTLNHPNIVTVFDVSVEGDQPFIASELIDGKPLRDE
                     IRRGHLPLKRALDLATQIADGLSAAHDAGIVHRDLKPENIMVTRNGRAKILDFGLTRI
                     GIGAPAGPAPNGAVTETEAGLRSGTVPYMSPEQALGSTSDFHGDQFSFGLILYEMLTG
                     RSAFRRDTPAATLHAIINDELPDMSALGARTPVQLRWIVERCLAKQPADRYASTADLH
                     RELRMLRERLTEIADRGASRSWPFTMWWRVALTAVASAAVVGTLIGLLVPLVGGEVVD
                     VSAVRFTPLATEAAYEGFASWSPTDEIIVYTADVGGVLQLFTRRLSSSQTAQVTFGNF
                     DATSPFWSHDGKRIYYISQAENSQGIWSVGAAGGTPEIVVRDALRGAISPDGTTIAFL
                     RDEGQGDIVGDSRLWMSTPGTIEPWSSDSVESHAVKSDALGNLPFVEGSLAFSPDGSR
                     LGICAVARSIELQPEARGWQFWIVPLKRGAPYRRLEGWADLAPRATNFSWLPDGHHVI
                     LSVISLARPGADLWIADLNRDRWWPLTRSADNEYYPSASPSGDQIVFTREDSDYDLVS
                     VALEPQATESRAAAIAPLRPLIVTARSESEPAWSADGSLLAYVTDRSGQDEIWLHSRQ
                     GERWVDRPVVTQKEFGDDLTIMLGAPTFSPDGHRIAFLRNAQKPMIWPLRIWTRFTDG
                     GAPVPLLPASHEGYQSAPTWSPDGQWIAYTEWKSRQWVLAKIRVGSGAEPIVLRSDGV
                     PGAAPHWSPLGDWITWETERGLILVSPDGTTERTLQGDHATDRWLFHEWAARGTRLAG
                     IKESENLQLVIVEVDPATGREKVLAHLGPSPPVNNPVKGFSLSADGRTAVIATVHLRG
                     DLWVMDGVKLRRRTLADWFAPALNFQ"
BASE COUNT          616 a         1102 c         1041 g          591 t
ORIGIN      
        1 gaacctggcg agcctgatca aggaagtccg gcgcgcactg gacgacaccg cagagactcc
       61 gcgtttcatc aggacggtcc atcgcttcgg gtattggttc atcggcgagc tacgcgagga
      121 ttccgcctcg cagacgccga cgcctcagtc ggtgaggtac tggctggttt ggctgtcgcg
      181 tcaaattccg gtccaggccg gcgaacacat cctcggccgg gcgcccgatg cttctgtgtg
      241 gattgacgca gcgggcgtgt cccgacaaca cgcccgactc gttgtcgacg atcacggcgc
      301 cacggtcgaa gatctcggca gcaagaatgg cacgttcgtc ggcgaccagc cggtcacggc
      361 cgcccggccg ctccttgacg gcgatcagat tcgtctcgga ccggttgtca tcacattccg
      421 catcccaccg ccggttgggg caacggacac tgtacagagt gactagattg gccgagcatc
      481 actgaaaatt gagcgcgggc gcgaaccagt cggccagagt tcttcggcgc aacttgactc
      541 catccatcac ccagagatcg ccgcggaggt gtacggtggc gatcacggca gtccggccgt
      601 cggcactgag cgaaaatccc ttgaccggat tgtttacggg cggcgacggc ccaagatgcg
      661 cgagcacttt ctctcgtccg gtcgcaggat cgacctccac gatgaccagc tggaggttct
      721 ctgattcctt gatgcccgcc agccgagtcc ctcgagcggc ccattcgtga aacagccagc
      781 ggtcggtcgc gtgatcgccc tgcagcgttc gctcggttgt tccatcggga gagacgagga
      841 tgagccctcg ttccgtttcc cacgtaatcc agtcgccgag cggcgaccaa tgcggggcgg
      901 cgccgggcac cccgtcgctt cgaagaacaa tcggctcagc gccgcttccg acccggatct
      961 tggccagcac ccactgccgc gatttccatt cggtgtaggc aatccactgg ccgtccggcg
     1021 accaggtcgg cgcgctctgg tagccttcgt gcgaagccgg cagcagcggc acgggcgcac
     1081 ccccgtcggt gaaccgagtc cagatccgca gcggccagat catcggcttc tgcgcattac
     1141 ggagaaaggc gatccggtgt ccatcaggcg agaacgttgg cgcgcccagc atgatcgtca
     1201 gatcgtcgcc gaattccttc tgtgtgacga ccggtcgatc gacccatctc tcaccctgtc
     1261 ggctgtgcaa ccagatttcg tcctgcccac tgcggtcggt cacgtacgca agcaggctgc
     1321 cgtcggccga ccacgccggc tctgattcgc tgcgagcggt cacgatcagc ggtcgcagtg
     1381 gcgcaattgc cgctgctcgg gactcagtcg cctgcggttc gagtgcgacc gagaccaggt
     1441 cgtagtcaga gtcctcgcgc gtgaacacga tctgatcgcc agacggcgag gccgatgggt
     1501 aatactcgtt atccgcgctt cgcgtcaacg gccaccaccg gtcacgattc aagtcggcaa
     1561 tccagagatc agccccaggt ctggccagag agatcactga caggatcaca tgatgcccat
     1621 cgggcaacca gctgaagttg gtcgccctcg gggcgaggtc ggcccagccc tcgagccgtc
     1681 ggtacggagc gcctcgcttc agcggtacga tccagaactg ccagcctcgc gcctcgggct
     1741 gcagttcgat cgagcgcgct actgcgcaga ttccgagccg acttccgtct ggcgagaagg
     1801 cgagagaccc ttcgacgaac ggcaggtttc cgagggcgtc ggatttgacg gcatgactct
     1861 cgacactgtc gctgctccac ggctcgatgg tgccaggcgt cgacatccac aatctggagt
     1921 cgcctacgat gtccccctgc ccctcgtcgc gcaagaacgc gatcgttgtg ccatcgggtg
     1981 agatggcgcc gcgaagcgcg tcgcggacaa cgatctctgg cgtaccacca gcggctccga
     2041 ccgaccagat gccttggctg ttctctgctt gcgagatgta atagatccgt ttgccatcgt
     2101 gcgaccagaa tggactggtc gcgtcgaagt tgccgaaggt cacctgcgcg gtctgcgacg
     2161 acgagagccg ccgtgtgaag agctgaagca cgccgccgac atcggccgtg tagacgatga
     2221 tttcgtcggt cggtgaccac gaggcaaatc cctcatacgc cgcctcagtg gcaagtggcg
     2281 tgaatcggac cgctgaaacg tccaccacct caccgccaac cagcggcacg agcaagccga
     2341 tcaaggtccc cactaccgcg gccgacgcga ccgccgtcaa tgccacccgc caccacattg
     2401 tgaacggcca tgaccgggaa gcgccgcgat cggcgatttc cgtcaagcgc tcgcgaagca
     2461 tgcggagttc gcgatggaga tcggccgttg acgcgtagcg atctgccggc tgcttggcga
     2521 gacaccgctc gacgatccac cgcagctgaa ctggcgtgcg cgcgccaagc gccgacatgt
     2581 ccgggagttc gtcgttgata atcgcatgca acgtcgccgc tggcgtgtcc cggcggaatg
     2641 ccgacctgcc ggtcagcatt tcgtacagga tgagtccgaa cgaaaactga tcgccgtgaa
     2701 agtcggacgt ggagccgagc gcctgttccg ggctcatgta gggaaccgtg ccggatcgaa
     2761 gaccggcttc ggtctcagtc accgcaccgt tcggcgccgg tcctgccggc gcgccgatgc
     2821 cgatgcgagt caatccgaaa tcaaggatct tcgcgcgccc gttgcgcgtc accatgatgt
     2881 tctcgggctt gagatcgcga tggacgatgc cggcatcgtg tgccgccgac agaccgtcag
     2941 cgatttgcgt ggccagatcg agcgcgcgtt tgagtggcag atggccacgg cggatttcat
     3001 cgcgcagcgg cttgccgtcg atcaactcgg acgcgatgaa cggctggtcg ccctcgaccg
     3061 acacgtcgaa caccgtgacg atattgggat ggttgagcgt gccagcggcc cgcgcctcgg
     3121 cgacgaaccg gtcgagccga ccggcatcga tcgccaatcg acggtgcaga atcttgagcg
     3181 cgacctcgcg ctcgagccgc ggatcccagg cccgatacac ttctcccccg cctccctgcc
     3241 cgatcaaatc gagaatccgg tagggccccg ccggcggccg aggagcagca tcaacgttca
     3301 tgaagcgctg atgatcttaa cgctgaccga cccgatgtga atcagcgccc
//
LOCUS       DATAOZ010000652         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2882872, whole genome shotgun sequence.
ACCESSION   DATAOZ010000652 DATAOZ010000000
VERSION     DATAOZ010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2882872"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VHR64_16320"
     CDS             complement(<1..920)
                     /locus_tag="VHR64_16320"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX3304453.1"
                     /translation="MTTATPRLSAADPLNLEAEYTDFERDLRDRTRAFAREHIAPHVQ
                     AWWDAESIPRDLIIEFGKLGIVGLMHQPGGDKHAIAYGLVNAELDAVDSGVRTLFSVQ
                     GGLAMTAIYRFGSDQQRAHYLPKMRTGEILGAFALTEPESGSDPGSMICTARRDGSDW
                     VLNGHKRWNTNCLIADIVITWVRTDEGIRGFIVPTDSPGLERRAVTGKHSLRAAVATE
                     FEMHDVRLPADAVLPGVTGLAGPLACLSEARYGIIWGMLGVMRSCLETALEHSTTRIQ
                     FGKPIGAYQLTQAKLTDMLLTYSQAQLLAH"
     gene            1115..1852
                     /locus_tag="VHR64_16325"
     CDS             1115..1852
                     /locus_tag="VHR64_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304454.1"
                     /translation="MVSREQILINGVPTRIHGSFERTVQDEAGRTVAEMEAVIIVRGR
                     LPNKQFQQQISRETIQLDYQDGQTPVTMVTRVVNHTAVASGEGEGTVYRHDITFRELP
                     ESYQQRMAKQASEAAAKALAAPRPSPRVAAQEPEPVGDISDIKTATDPSNWGDAIRQL
                     RGSMPKPQAPEEPLTMPELTGIETVLVNLRLEALIHVLESTGCIPHGAAEIQFRELLE
                     ERFFEDASQIVGEGVAQRALREMHTSH"
BASE COUNT          397 a          636 c          771 g          352 t
ORIGIN      
        1 tggtgggcga ggagctgggc ctggctgtac gtgagcagca tgtcggtcag cttggcctgc
       61 gtgagttgat aggcgccgat cggcttgccg aactggatgc gcgtcgtgct gtgctcgagt
      121 gccgtctcca gacagctccg catcacgccg agcatgcccc agatgatccc gtagcgggcc
      181 tcgctcaggc aggcgagcgg tccggcgaga ccggtcacgc cggggagcac ggcatcggcc
      241 gggaggcgga cgtcgtgcat ctcgaactcg gtcgcgacgg ccgcgcgcag cgaatgcttg
      301 ccggtgaccg cgcggcgctc gagcccgggt gagtcggtcg ggacgatgaa gccgcggatg
      361 ccctcatcgg tccggaccca cgtaatgacg atgtcggcga tcaggcagtt agtattccag
      421 cgcttgtggc cgttcaggac ccagtcggag ccgtcgcggc gggcggtaca gatcatgctg
      481 ccaggatcgg agccggattc gggctcggtc agggcgaagg cgccgaggat ctcgccggtg
      541 cgcatcttcg gcaggtaatg ggcgcgctgc tggtccgagc cgaagcggta gatcgcggtc
      601 atggccagac caccctggac cgagaagagg gtgcgcacgc cgctgtcgac cgcgtcgagc
      661 tcggcgttga cgaggccgta ggcaatggcg tgcttgtcgc caccgggctg gtgcatcagg
      721 ccgacgatgc cgagcttgcc gaattcgatg atcaggtcgc gcggaatcga ttcagcgtcc
      781 caccatgcct gcacgtgcgg agcgatgtgc tcccgggcaa aagcgcgggt cctgtcgcgc
      841 aggtcgcgct cgaagtcggt gtactcagcc tcgaggttga gcggatcggc ggcggagagg
      901 cgaggggtgg cggtcgtcac gggactccct tcctatatat ggtggcggat ataccggaaa
      961 gtgtagagat cgccgggccg cagtgcccgg tcccgattac ccgaacagcg ggaacggtcg
     1021 gtacaagtgg tggactgtcc gttcagtggt cgccccgtag gataaagcca cggattccgc
     1081 cgggtggcgg tgcgcaggcc tccggaggga cggcgtggtt tcgcgggagc agattctgat
     1141 caacggggtg ccgacccgca tccatggcag cttcgagcgc acggtgcagg acgaggcggg
     1201 ccggaccgtg gccgagatgg aggcggtcat catcgtccgc ggtcgtctcc cgaacaagca
     1261 gttccagcag cagatcagcc gcgagacgat ccagctcgac taccaggacg gccagacgcc
     1321 ggtcacgatg gtcacgcggg tggtcaacca cacggcggtc gcgagcggag aaggtgaggg
     1381 gacggtctac cgccacgaca tcaccttccg ggagctgccc gagtcgtacc agcagcggat
     1441 ggcgaagcag gcgtcggaag cggccgccaa agcattggcc gcgccccggc cctcgccacg
     1501 ggtagctgct caggagccgg agccagtcgg cgatatctcg gatatcaaga ccgcgacgga
     1561 cccgtcgaac tggggcgatg cgatcagaca gctcaggggc agcatgccga agccgcaggc
     1621 gccggaagaa ccgctgacga tgccggagct gaccgggatc gaaacggtgc tggtcaactt
     1681 gcgtctggag gcgctgatcc atgtgctcga gtcgaccggg tgcatcccgc acggggcggc
     1741 ggagatccag ttccgggagt tgctggaaga gcgcttcttc gaagacgcaa gccagattgt
     1801 cggcgagggg gtcgcccaac gcgccctccg ggagatgcac acgagtcatt agttttgaga
     1861 cgtacgtcag ttgatggtcg ttggtcgttg gtagatggac acggaatacg gaaaaccgaa
     1921 aacggagaac cagataccaa agtcgagaga cctcgagcgg aagtctgacg tgcgcactca
     1981 gttttgagaa tgaatctcat tatcgggtat aattgaacag cggttcggtg gggccggatc
     2041 gcttcgtttt cgctcgcgac acgcaccccg tttgatcgga tcacagatgg cgcaatcgct
     2101 ccaggcctcc atgcggctcc cggtgacgaa accggcccag cccgcacgct cgctcc
//
LOCUS       DATAOZ010000653         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2360664, whole genome shotgun sequence.
ACCESSION   DATAOZ010000653 DATAOZ010000000
VERSION     DATAOZ010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360664"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1032)
                     /locus_tag="VHR64_16330"
     CDS             complement(43..1032)
                     /locus_tag="VHR64_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304455.1"
                     /translation="MDRVTNILTSIPGYDGYRDKENRRETDKRVRDRISTRLGDLARG
                     IELVATDLANKRDIQAVGPVDSAAKSVRHLQNLVSTATYGYGGLLGDRNVDEVALEQL
                     NQFDADLLSRVETLAPLVEQLGAATDAAGREAALGATRTAVSELQTRFEARSSVVETG
                     RPTMPAASTSPLTVLEQESTKPLKPAVLTLEQGHALSIGGTNYIVDAVIDIGGTQPMR
                     LYRIDVAPERWLLANERFAAMLSPGQFSDQAQEVEVDGQSLTSHGSGSAPSTVTGLSG
                     KSGQQTVTYRVYGGATESGPLALTLTWPAAEMRLVGRGMALDDIEVFGKPDDR"
     gene            complement(1050..1571)
                     /locus_tag="VHR64_16335"
     CDS             complement(1050..1571)
                     /locus_tag="VHR64_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304456.1"
                     /translation="MRSSAKTVGSLMAGGGAILLIAVLAWGLLGKSNGDLETTGFVLL
                     LILALIVLLPLILGGLFMIFRGLAETKELAEVDKQRKLLNLVQTRGQVSISDLVLDMQ
                     STRDAVQHSLTELVGRGLFSGYVDWQKGMLYSVEASQLQGRQTCPNCGGQLQLAGKGI
                     IKCPYCGAEIFLS"
     gene            complement(1613..>2249)
                     /locus_tag="VHR64_16340"
     CDS             complement(1613..>2249)
                     /locus_tag="VHR64_16340"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX3304457.1"
                     /translation="VIHHGISADFAPATEPEIARVRATWSLPDRFVLAVGTIQPRKNL
                     ARLAQAVAALLGEIPDLALVVAGKRGWMADTVLDEISAALPHEYFRELGYVPLTDIPA
                     LYSTAAVTALVSTYEGFGLPVIEAMASGSPVVVSDTPALTEIAGKAALVAEARSVTGI
                     ADALRAVLSDQSKATRLTTSGRAHAASFSWDKAARETVDLLHRMLENRAPR"
BASE COUNT          415 a          704 c          709 g          421 t
ORIGIN      
        1 gcgtgagagt cgatcgagaa tacccatggt ctttcgctcc ttctaccgat cgtcaggttt
       61 gccaaacacc tcaatatcgt cgagggccat tccacggcca accaggcgca tttccgcggc
      121 tggccatgtg agtgtaaggg ccagcgggcc cgattctgtt gccccaccat acacgcgata
      181 ggtaacggtc tgctggccgg acttgccgct gagcccggtc acggtcgacg gagccgagcc
      241 actgccgtgc gatgtcagcg actgcccatc gacctcgacc tcttgtgcct gatcggagaa
      301 ttgcccgggc gagagcatgg ccgcgaagcg ctcgttggcc agaagccagc gctccggcgc
      361 gacatcgatt cgatagagcc gcattggttg cgtaccgccg atgtcgatga cggcgtcgac
      421 gatgtagttt gtgccgccga tcgagagtgc atgcccctgc tcgagtgtga ggacggccgg
      481 cttaagcggc ttcgtcgact cctgctcgag gaccgtgagc ggcgaagttg aggcagccgg
      541 cattgtgggc cggcccgtct cgaccacgct ggatcgtgct tcgaacctgg tctggagctc
      601 agaaaccgcg gtgcgagtcg ccccaagtgc cgcttctctc ccggcggcat cggtcgcggc
      661 gcccaactgc tcgaccagtg gcgccagcgt ctcgacgcgg gacaggagat ccgcgtcaaa
      721 ctggttgagc tgctcgagtg cgacctcgtc gacattgcga tcgccgagga ggccgccgta
      781 gccataggtc gccgtcgaga cgaggttctg gaggtgacgg acagacttgg cggcgctgtc
      841 caccggtccg accgcctgga tgtcccgctt gttagcgaga tcggtcgcga ccagctcaat
      901 gccgcgggcg agatcgccga ggcgggtgct gatgcggtcg cggacgcgct tgtcggtctc
      961 gcggcggttt tccttgtccc ggtaaccgtc atatccgggt atcgaagtga ggatgttggt
     1021 gactcgatcc atgtcgattg cctcccgcgc tatgacagaa agatctcggc tccgcagtaa
     1081 ggacacttga tgatgccctt gccggcgagc tgcagttggc cgccgcaatt cgggcaagtc
     1141 tgacggccct gcaactggga cgcctctacc gagtagagca tgcccttttg ccagtccacg
     1201 tacccgctga acaatccgcg ccccacaagc tcggtcaagg aatgttgaac ggcgtcacgg
     1261 gtcgactgca tatccaggac aagatcgctg atcgaaacct ggccgcgagt ctggaccagg
     1321 ttcagcagtt tgcgctgctt gtcgacttcc gccagctcct tcgtctcagc caggccgcgg
     1381 aagatcatga acaggccacc caggatcagg ggcagcagca cgatcagcgc caggatcagg
     1441 agcagcacga agccggtcgt ttccagatct ccgttggatt tgccgagcag accccatgcc
     1501 agaaccgcga tgaggagaat tgcgcctcct ccggccatga gcgacccgac tgtctttgcc
     1561 gatgaccgca cgagattcct ccctgccaaa cgatccgaat agtacgcaat cgttaccgcg
     1621 gcgcgcgatt ctccagcata cggtgcagaa gatctacggt ctcccgggcg gccttgtccc
     1681 aggagaatga cgcggcatgt gctcgccctg acgtcgtaag tcgcgtcgcc ttgctttggt
     1741 cggacaacac tgccctcaac gcgtcggcga tgccggtcac tgatcgcgcc tcggcgacga
     1801 gtgcggcctt cccggcgatt tctgtcaggg ccggtgtatc ggagacgacg accggcgagc
     1861 cgctggccat cgcctcgatg accggcaatc cgaagccctc gtatgttgaa acaagcgccg
     1921 tgaccgcggc cgtggaatag agagccggaa tgtcggtcag gggaacatat cccaattcgc
     1981 ggaaatattc gtgtggcagg gcggcactaa tctcgtccag gaccgtatca gccatccaac
     2041 ctcgctttcc agctacgacg agggccagat cgggtatctc gcccagcagc gcggcgacag
     2101 cctgcgcaag tcgggcaagg ttcttgcgcg gctgtatggt cccgacggcg agcacgaacc
     2161 ggtcgggcag ggaccaggtc gcgcgaacgc gagcaatctc gggttcggtc gccggcgcga
     2221 aatctgctga gattccatga tggatcacg
//
LOCUS       DATAOZ010000654         4712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_258893, whole genome shotgun sequence.
ACCESSION   DATAOZ010000654 DATAOZ010000000
VERSION     DATAOZ010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258893"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VHR64_16345"
     CDS             <1..965
                     /locus_tag="VHR64_16345"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEX3304458.1"
                     /translation="FAVLANLKGTTIEDGAETPFYDADAQEIGTVTVDDIITDFQKSD
                     SEITPARGMTTIAVSLTIAATGETDLQPNSYSFSLVDDFGFLYYPQFYFRSEESTAQY
                     PDLPTDTLAAGSDASGVVLFEIPKDAQISYITYAPDYTQFYILAQPGPGSTVSGDTLT
                     PVANPTSSGDDTPEATEDTGSTGGTETGDCVGVSDWAKATEEVLAPISDVLNGSDSLN
                     DVNPDDLRDAANQMRDAADAQADVETPEIAQGANDAIVDMLNGFAGFYDDIADRLDKG
                     DSPEDIQADLENDPELENLFTPFVNELTTLMTSCPDSDLSGIFG"
     gene            complement(1110..1727)
                     /locus_tag="VHR64_16350"
     CDS             complement(1110..1727)
                     /locus_tag="VHR64_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304459.1"
                     /translation="MSSYYDAEVEPELPDEHQFTPFPDQEVTDAGAYMCHHSGEPPVE
                     HVEHPSTTDLRQEAVAAVLAARAGREDEVCIECERLVNEQVRLAWHAYVRAKVEFYRA
                     RRGRAARPVETVSLEESGLDFEIPEEDEDDEEIPVESLMVVRQIVRERVPGLRVQQAV
                     RIVTVMQEVQTIKRKKRREPVPDATRAELARLRKATGLPLDTGLL"
     gene            complement(1871..2803)
                     /locus_tag="VHR64_16355"
     CDS             complement(1871..2803)
                     /locus_tag="VHR64_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX3304460.1"
                     /translation="MEIRLITTESVAGTAGSPDPERAAALAGAAAAFLQAGIVERLAE
                     AGATVDQVVSVSAPKGSADDPIAALAGINARVGRAVAAALRQDVAPVLVGGNCSHTIG
                     MIAGIQAAYGPTTRLGLLWLDAHGDFNTPRTSHSGMLGGMPVAVAAGLCWPEWREGAG
                     QQVPLPTSRIVMVDVRNLDEAEEALIRSTDVEVVRFGEGFDVAPILEGIARLAAQCDQ
                     IYVHVDADILDATLQPNHPTVEPGGPGVEPVVKVLNAAFETGKVCAFAVVSVNPNGPD
                     GKISVESAGELLVRGARRWDAVRAAEPTWVMRSE"
     gene            3152..4213
                     /locus_tag="VHR64_16360"
     CDS             3152..4213
                     /locus_tag="VHR64_16360"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEX3304461.1"
                     /translation="MSLTRRSLLAGGAALSSAALLTGRISATGIELDGSPTPWASPVG
                     GEMVVYSGRNEALVGPLLEEMKSDIGVDISVRYGNTAELAATILEEGKNSPADVYFAQ
                     DAGALGALAKAGVLAPLPQELLDKVDQRFRSKDGLWIGTSARARVLAYNTENVDPATL
                     PNTVEALVDPKWSGKLGWAPPNASFQAFITAFRLLRGDDAARKWLEGMMANNMTAFDN
                     NADAVRAVARGELAGALVNHYYKYEITREEGHDIPVENHFFDPGDVGSLVNVAGVAIL
                     KTTKHAEQADAFTRFLLDVEAQEYFAKETAEYPVADGVKPYYDDLPELDKVHGPDIDL
                     NDLDDLEGTLEMLTDLGLL"
     gene            4514..>4712
                     /locus_tag="VHR64_16365"
     CDS             4514..>4712
                     /locus_tag="VHR64_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304462.1"
                     /translation="MVASTIEQQPQTTAISARPRGRSTRRAPLPLVIAALLVALFMLA
                     PAIYLGIRASDAGTEAFTILKR"
BASE COUNT          855 a         1750 c         1306 g          801 t
ORIGIN      
        1 agttcgccgt gctcgccaac ctcaagggca cgactatcga ggacggcgcc gaaacgccct
       61 tctatgacgc cgacgcccag gagatcggca ctgtcaccgt cgatgacatc atcaccgact
      121 tccagaagtc cgattccgag atcaccccgg cccgcggcat gaccaccatc gccgtctccc
      181 tcacgatcgc cgccaccggc gagacggacc tccagccgaa ctcctacagc ttctcactcg
      241 tcgacgactt cgggttcctc tactacccgc agttctactt ccgcagcgaa gagtcgaccg
      301 cgcagtaccc ggatctcccg acggacacac tggccgccgg tagcgatgcc tcgggcgttg
      361 tcctcttcga gatcccgaag gacgcccaga tctcctacat cacctacgcg cccgactaca
      421 cgcagttcta catcctcgcc cagccgggtc ccggctcgac cgtctccggt gacaccctga
      481 ccccggtcgc gaacccgacc agcagcggcg acgacacccc agaggccacc gaagacaccg
      541 gcagcaccgg cggcacggaa accggcgact gcgtcggcgt cagcgattgg gccaaggcca
      601 ccgaggaggt cctcgctccg atcagcgacg tcctcaacgg ctcagactcg ctcaacgacg
      661 tcaatccgga cgacctccgc gatgccgcta atcagatgcg cgatgcggca gatgcccagg
      721 ccgacgttga gacgcccgag atcgcccagg gcgccaacga cgcgatcgtc gacatgttga
      781 acggcttcgc cggcttctat gacgacatcg ccgatcgcct cgacaagggc gacagtccgg
      841 aggacatcca ggccgatctg gaaaacgatc cggagctcga gaacctcttc accccgttcg
      901 tcaatgagct gaccacgctc atgacctcct gcccggactc cgacctgagc ggcatcttcg
      961 gctaacccaa ccgggcaacc gaatcgtcac gaagaccggg tggagcaaat gctctacccg
     1021 gtctttggtc tttcgtcttt ggctttcggt ttttcgtttt ccgtatccat ctaccaacga
     1081 ccaaccacca tcaaccctcc accctcaact cacaaaagtc ccgtatccag cggcaacccg
     1141 gtcgccttcc gcaaccgcgc cagctccgcc cgggtcgcat ccggcaccgg ctcccgccgc
     1201 ttcttgcgct tgatcgtctg cacctcttgc atcaccgtca cgatccgcac cgcttgctga
     1261 acccgcagtc caggcacccg ctcgcggaca atctgccgca cgaccatgag cgattcgacc
     1321 gggatctcct catcgtcctc gtcctcttcg ggaatctcga aatcgagccc gctctcttcc
     1381 agcgagacgg tttcgaccgg acgcgccgcg cgaccgcggc gggcccggta gaactcgacc
     1441 ttggcccgta cataggcatg ccacgccagc cggacctgct cgttgaccag tcgctcgcac
     1501 tcaatgcaaa cctcgtcctc gcgaccggcc cgcgccgcca ggaccgcggc gaccgcctcc
     1561 tgccgcaaat cggttgtcga cgggtgctcg acatgctcga ccggcggctc gcccgagtga
     1621 tggcacatat atgccccggc atcggtgacc tcctggtcag gaaacggcgt gaattggtgc
     1681 tcgtcgggga gctccggctc gacctcggcg tcgtaatagc tggacatggg acggggtcct
     1741 ctcgcaatga gatcaagcgc ctggaggtgt gggaacacga cggggcgcaa atgatcggcc
     1801 tgtcactgac cccagaatag aacttatgtt ctatttccgc aagcaggaag caggtcccat
     1861 gcccctcggc ctactccgac cgcatcaccc acgtcggctc ggccgcccgc accgcatccc
     1921 agcgtcgcgc accccgcacc agcagctcgc ccgcactctc gaccgagatc ttcccatccg
     1981 ggccgttcgg attgaccgac acgactgcga atgcgcagac cttgcccgtc tcgaacgccg
     2041 cgttcagcac cttgaccacc ggctcgaccc cgggaccgcc cggctcaacg gtcggatggt
     2101 tcggctgcaa ggtcgcatcg aggatgtcgg catcaacatg gacgtagatc tggtcgcatt
     2161 gagccgccag ccgggcgatc ccctcgagga tcggcgcgac gtcgaatccc tcgccaaagc
     2221 gcacgacttc gacatccgtg gaccggatca gcgcctcctc ggcctcatcc aggttgcgca
     2281 catccaccat cacgatccgg ctcgtcggca gcgggacctg ctgccccgca ccctcccgcc
     2341 attccggcca gcaaagaccg gccgcaaccg ccaccggcat cccgcccagc attcccgagt
     2401 gcgaggtccg cggtgtgttg aaatcgccgt gcgcatccag ccagagcagt ccgagccggg
     2461 ttgtcggccc atatgccgcc tgaattcccg cgatcatccc gattgtgtgg ctgcaattgc
     2521 cgccgaccag caccggcgcc acatcctgcc gcaacgctgc cgccaccgcg cggccgaccc
     2581 gcgcgttgat acccgccagc gccgcgatcg gatcgtcggc cgaccctttc ggcgcggaga
     2641 ccgacaccac ctgatcgacc gtcgccccgg cctcagccag ccgctcgacg atgcccgcct
     2701 gcaggaaggc cgcggccgcc ccggccagcg cagccgcccg ctccggatcc ggactccccg
     2761 ccgtccccgc cacactctcc gtcgtaatca gccgaatctc catgtggtcg cttcgctcca
     2821 gacttcagac gtcagacttc ggacttcagt ttccagtttt tggtttttag tttccagcga
     2881 ggtctttggt ctttcgtctc tcggtttcgg gtttcgggtt tcgtctctcg gtttcgggtt
     2941 tcggaggaga gcgtaactgc tcgcctccct cacgtcgcgc ctccgcgcct tctcgtctcc
     3001 gcgccccagc tgctcgccta ctcgcctttc ccaattccga ccactctgct aggattaaac
     3061 ctgaccggct tggtccgaat tggatcagcc ttccgaaaga cggcgcatgg gcgccgtcgt
     3121 ttcatgtcac cccctcccaa ggaggtttcg catgtcgttg acccgtcgtt cgctgcttgc
     3181 cggcggtgca gcgctttcgt ccgctgcgct gctgaccggc cgcatctccg ccaccgggat
     3241 cgagctcgat ggcagcccaa ctccctgggc atcgccggtc ggcggtgaga tggtcgtcta
     3301 ctccggccgc aacgaagccc tggttggccc cttgctcgag gagatgaagt ccgatatcgg
     3361 cgtcgacatc agcgtccgct acggcaacac tgccgagctg gccgcgacga tcctcgagga
     3421 gggcaagaac agtccggccg acgtctactt cgcccaggat gccggcgcgc tcggcgcgct
     3481 ggccaaggcc ggcgtcctcg cgccactgcc gcaggagttg ctcgacaagg tcgaccagcg
     3541 cttccgctcg aaggacggac tctggatcgg cacctccgcc cgggcccgcg tcctcgccta
     3601 caacaccgaa aacgtcgatc cggccacgct gccgaacacg gttgaggccc tggtcgatcc
     3661 gaagtggtcc ggcaagctcg gctgggcgcc gccgaacgcc tccttccagg ccttcatcac
     3721 tgccttccgc ctcctcaggg gtgatgacgc cgcccgcaaa tggctcgagg gcatgatggc
     3781 caacaacatg accgccttcg acaataacgc cgacgccgtc cgcgcggtcg cccgcggcga
     3841 gctggccgga gcgctggtca accactacta caagtacgag atcacccgcg aggaaggcca
     3901 cgacatcccg gtcgagaacc acttcttcga ccccggcgat gtcggctcgc ttgtcaacgt
     3961 cgccggcgtc gcgatcctca agacgaccaa gcacgccgag caggccgatg ccttcacccg
     4021 cttcctgctc gatgtcgagg cgcaggagta cttcgccaag gagaccgccg aatacccggt
     4081 cgccgacggc gtcaaaccct actacgacga tctcccggag ttggacaagg tccacggccc
     4141 ggacatcgac ctcaacgatc tcgacgacct cgaaggcacc ctcgagatgc tgacagacct
     4201 cgggctcctg tgaggcgagc aggcgagcag taacggcacg gagacgggga ggcgcaaagt
     4261 tctcccctct cccgcttgaa aaggggcggg agaggggccg ggggtgaggg ttaccactct
     4321 ttcgtaacgg cccctcatcg cctcgcccgg gaatgaattc ccgctctgac aaacggatga
     4381 cggttgaaac cgacacaatc ggccgggatc attcccctgg ccgcacaatc acggccgact
     4441 cggaaaaccg acaacggaaa actcgctagg aactagaaac gagaaactgg aaactccgtc
     4501 actgaccaca gccatggtag ccagcacgat cgagcaacaa ccgcagacaa ccgcgatcag
     4561 cgcccgcccg cgcggtcgat cgacccgtcg tgccccgctt cccctcgtta tcgcagccct
     4621 tctggtcgcg ctttttatgc tcgccccggc gatctacctc ggcatccgcg catcggacgc
     4681 tggcaccgag gccttcacga tcctgaagcg gc
//
LOCUS       DATAOZ010000655         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4726835, whole genome shotgun sequence.
ACCESSION   DATAOZ010000655 DATAOZ010000000
VERSION     DATAOZ010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4726835"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..503
                     /locus_tag="VHR64_16370"
     CDS             66..503
                     /locus_tag="VHR64_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304463.1"
                     /translation="MQQSAGMAGSNQEAATVNPDAPRIARILIVARPALSRALSSTLS
                     VAGYEVHRTPDAASAVEAARRLRPEVAIVASEIPGSSGDVVAQLLRASRVNLPVILLG
                     ESPAPAELEAIVGVPRDVTPAELLAVVAQQLPSNPEDDRPNAG"
     gene            493..1812
                     /locus_tag="VHR64_16375"
     CDS             493..1812
                     /locus_tag="VHR64_16375"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX3304464.1"
                     /translation="MPDRASAHSRSIPVVTADEAMDHIRSGDRIYLHEAAMAPLELME
                     ALVRRASDLRDVETVSIHTEGPAPHTAPGLEGHIRHNALFVGSNVRQAVNEGRADYTP
                     VFLSDIPSLFAGHDLPLDVAFVQVSPPDHHGFCRLGVSVACARAAVDHARIVIGLINP
                     QVPLTLGNSAVHVSRFAALVETDRPLPTLTPGPISEISRRIGEHVASLIPNRATLQLG
                     IGAIPDAVLSCLDQHEDLGIHTEMFSDGLLALAERGVITNRYKRTWKGRVVTSFALGS
                     QRLYDFIDSNPFVEFHSSDIVNDTREIRRNDNMIAINSALEIDLTGQVVADSIGEYIY
                     SGIGGQMDFMRGARLAQGGKAIISLPSTAKSGTVSRIVPTVKSGSGIVTTRGHVEYVA
                     TEFGVVDLAGQSLRRRAELLISIAHPDFRPDLESAARARHYSVPTPA"
     gene            1944..2441
                     /locus_tag="VHR64_16380"
     CDS             1944..2441
                     /locus_tag="VHR64_16380"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX3304465.1"
                     /translation="MFSRIVVPLDGSPLAETALDQATELARLSGAEIRLVRVVDFTMT
                     EKHGAFGLALEYAPQQFFDAEQDDAVTYLRDTADRLRGSGISVSYEVTRGRVAQILAN
                     YCQPGDVVVMASHGRGGLSRWFLGSVAEDLVRNSTVPVMLVRAQNSVAPEPLAVPASS
                     NAVLV"
BASE COUNT          428 a          832 c          758 g          469 t
ORIGIN      
        1 acccatctgc ccgaccggca actgtgaata aggacgctcg cgcgactggt tatgcccagc
       61 agccagtgca acaatccgcc ggtatggcgg ggtcaaatca ggaagctgcg actgtgaatc
      121 cggatgcccc tcgtatcgcg cggatattga tcgtggcacg cccggcactg agccgcgccc
      181 tttcatcgac cctcagcgtc gccggttacg aggtccatcg gactccggac gctgccagcg
      241 cagtggaagc tgcaagacgc ctgcgacccg aagtggcgat cgtcgccagc gaaattcccg
      301 gctcgtcagg tgacgttgtt gcgcaactct tgcgcgcaag ccgcgtcaac cttcctgtta
      361 tcctcttggg cgaatctccg gcgccggctg aactcgaggc catcgtcgga gtaccgcgcg
      421 atgtgacccc agccgagctg ctggcggtcg tcgcgcaaca gttgccgagc aatcctgagg
      481 atgatagacc gaatgccgga tagggcgtcc gcacactcgc gatcgatccc ggtcgtcaca
      541 gccgacgagg caatggacca tatccgatcg ggcgatcgga tctatctcca cgaggccgcg
      601 atggcccccc tcgagctcat ggaagcgctc gtccgtcgcg catccgatct tcgcgatgtc
      661 gagacggtct cgatccacac cgagggtccg gcgccgcaca ccgcgcctgg tctcgaaggg
      721 catattcgcc ataacgccct cttcgtggga tcgaatgtcc ggcaggcggt caacgagggg
      781 cgggccgact acacgccggt gttcctgtcc gatatcccgt cgctcttcgc cggccacgac
      841 ctgccgcttg atgtcgcgtt cgtccaggtg tcgccccccg atcaccacgg gttctgccgc
      901 ctcggcgtct cggttgcctg cgcgcgagcc gcggtcgacc acgcccggat cgtgatcggc
      961 ctgatcaacc cgcaagtgcc gctgacactg ggcaactcgg cagtgcatgt cagtcgcttt
     1021 gccgcgctcg tcgaaacgga tcggccgctt ccgacgttga cgccggggcc aatcagcgag
     1081 atctcgcgac gcatcggtga gcacgtcgca tcgctgatcc ccaaccgcgc cacacttcag
     1141 ctcggcatcg gagcgattcc ggatgcggtg ctgtcatgcc tggaccagca tgaagacctc
     1201 ggcatccaca ccgagatgtt ctcggacggc ctgctcgcgc tcgcggagcg gggcgtgatc
     1261 acgaaccgtt acaagcggac ctggaagggc cgggtggtga cctcgtttgc cctcggcagc
     1321 cagcggctct acgacttcat cgacagcaac cccttcgtcg agttccactc gtcggatatc
     1381 gtcaacgata cgcgcgagat cagacgcaac gacaatatga tcgcgatcaa ctcggcgctc
     1441 gagatcgacc tgaccggcca ggtggtcgcc gactcgatcg gcgagtacat ctacagcggc
     1501 atcggcggtc agatggactt catgcgcggc gctcgcctcg ctcaaggtgg caaggcgatc
     1561 atcagcttgc cgtcgacggc gaaaagcggc accgtttcgc gcatcgtgcc gacggtcaag
     1621 tcgggctcgg gcatcgtcac cacccgcggc catgtcgaat atgtggccac agagttcggt
     1681 gtggtcgatc tggccggtca aagcctgcgc cggcgagcgg aactcctgat ctccatcgcc
     1741 catcccgact tccgaccgga cctcgaatcc gccgccaggg cgcgccatta ctcggtcccc
     1801 acgcccgcct gagcctccca tcgatcatct atcgcctgtt gcggatttgt gatacggcaa
     1861 tgctcttcct caatgcctgc tccatggccg acgcggcacg atagaggcac gttgaattgg
     1921 cctgcatcgc tgcggaggtt gagatgtttt cccgaatcgt tgttcccctg gacggctccc
     1981 cattggctga aactgccctt gaccaggcca ccgagttggc ccggctgtcg ggcgccgaga
     2041 tccgccttgt ccgcgtcgtc gacttcacca tgacggaaaa acacggcgcg ttcggcctgg
     2101 cactcgaata tgcgccccag cagttctttg acgctgagca ggacgacgcc gtgacgtacc
     2161 tgcgcgatac ggccgatcgc ctccgcggct caggcatcag tgtctcgtac gaggtcactc
     2221 gtggtcgggt cgcccagatt cttgccaact actgccaacc cggcgatgtg gtcgtgatgg
     2281 cgtcacatgg tcgcggtggc ttgtctcggt ggttcctggg cagcgtggct gaggacctgg
     2341 tgcgcaattc gacggtcccg gtgatgctcg tgcgagcgca aaactccgtg gcacctgaac
     2401 cgcttgcggt cccggcgtcg agcaacgcgg tcctggtcta aagccgcgcg aatggcgccc
     2461 gtagtggagg tgcgccgatg ttccaat
//
LOCUS       DATAOZ010000656         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_516474, whole genome shotgun sequence.
ACCESSION   DATAOZ010000656 DATAOZ010000000
VERSION     DATAOZ010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516474"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..746)
                     /locus_tag="VHR64_16385"
     CDS             complement(348..746)
                     /locus_tag="VHR64_16385"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEX3304466.1"
                     /translation="MLHEAYGFGADWAADAEPGMIVGMWEPRVGYTPDASIAWQLLVG
                     DETAVPAIGAILEALPAGARATVIIEVADRLDEIELPTCGDVDLRWLHRQPGTHGAPD
                     LLMNAVREQAFRGPGLRLGGRNRLHHPAEP"
     gene            complement(715..1071)
                     /locus_tag="VHR64_16390"
     CDS             complement(715..1071)
                     /locus_tag="VHR64_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304467.1"
                     /translation="MSRTRAGSENVVRCAFGDDLAILHRDQPVGVAGGEVGIVQDDHD
                     GPVLAPVQFMTLIVPLAGQERPMIEPVGFNWLDFQKIPEEVRPIARSYPVRRVYPERG
                     RTGDRLRAARGVWLRG"
BASE COUNT          273 a          523 c          499 g          264 t
ORIGIN      
        1 ggatgaacgc caccatccgc caaccggcgg gcgtgacctg gtagggagaa tcacacgcga
       61 ccgggctggc ttcagcccgg tcgcgtgttt gggatcgatt tgacggtcga ccgcgcacat
      121 ttgccgtcgt gttgaaccgc agcgaaacat ccccctgcga agccgctcgt tcgagaacct
      181 atagttcgtc tcccgacgaa gcgctttgtg cggggatcct tcccttcggt cgggatgatg
      241 gaagggaagg agacgcgagg gctgagaccc ggagccaggc gccgtcgcgg cgccaataac
      301 cgatggcgct gaccgagggg gtgttcaggc cgcgatcgcg gcggagctca aggttcagcc
      361 gggtggtgaa gccgatttcg cccgcccaga cgtagaccgg gccctcggaa ggcttgctcg
      421 cggaccgcgt tcatcaggag atcgggcgcg ccgtgcgtgc ccggctggcg atgaagccag
      481 cgcaggtcga cgtcgccgca cgtcggcagc tcgatctcgt ccaggcggtc ggcgacctcg
      541 atgatcaccg tcgcccgcgc gccggcggga agcgcctcca gaatcgcgcc gatcgccggc
      601 acggcggtct catcaccgac gagcaactgc caggcgattg acgcgtccgg ggtgtagccg
      661 acacgcggct cccacatgcc gacgatcatc cccggctcgg catcagccgc ccaatcagcc
      721 ccgaagccat acgcctcgtg cagcacgaag tcgatctcca gttcgccccc gctccggata
      781 gacgcgacgc accgggtagc tccgcgcaat cgggcgcacc tcctccggaa tcttctggaa
      841 atcgagccag ttgaagccga ctggctcgat cattggccgc tcctgcccgg cgagcgggac
      901 gatcagcgtc atgaactgaa ccggagccag gaccggacca tcgtgatcat cctgcacaat
      961 cccgacctcg ccgcccgcta cgccgacagg ctgatcgcga tgaaggatgg ccagatcgtc
     1021 gccgaaggca caccgaacga cattctcact ccccgccctg gtgcgcgaca tcttcgccgt
     1081 cgacgccgcc atgaccctcg acccggtcgt cggcacgccg ctggtgctgc cggaatcgac
     1141 ggtgcgcaag gaaatggttc tgctggcgag ctgagttgtg agttgtgaag aaagtgtagc
     1201 ggggctttag agagccggga gctgaagcac ccggctgaca gggtgaagta cgctgaagcg
     1261 cactgagcat ggttgattgc gatgccgccc ggatcagtct cgtagagact tcgtcttgtc
     1321 agcactggac ttcagtccgg tgcgacggct cccggcctcg tcgccgcact gtcggcctct
     1381 ggagcatccc gtctcggtcc ccggcactcg gccctcggta cttcgtcact gctcccgtat
     1441 gatgttcagg gtcactggcg accgttgatc gcatcgcaag ggagcgcgtc catgccgatt
     1501 ccgctgctcg atccgaacct gcgcgccggc tgggagccgg aaaccccatc ttccgattc
//
LOCUS       DATAOZ010000657         8665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1839343, whole genome shotgun sequence.
ACCESSION   DATAOZ010000657 DATAOZ010000000
VERSION     DATAOZ010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839343"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="VHR64_16395"
     CDS             <1..176
                     /locus_tag="VHR64_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HEX3304468.1"
                     /translation="NKIQVIKAVRELTSLGLKEAKDLVEAAPKPVKEGVSKDEANAAK
                     AKLEEAGAKVEVK"
     gene            299..375
                     /locus_tag="VHR64_16400"
     tRNA            299..375
                     /locus_tag="VHR64_16400"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Pro,seq:ggg)
     gene            409..1053
                     /locus_tag="VHR64_16405"
     CDS             409..1053
                     /locus_tag="VHR64_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3304469.1"
                     /translation="MQSNTQSRAPRRILIADDESLIRLDLREMLNHLGYEVVGEAGDG
                     RQAVELARKLKPDLVVMDIRMPEVDGIQAAGILTSERIAPVVLLTAFSDTELVEQARE
                     AGVVGYVVKPFREAELMPVIELSFARFEEFRALEKEIGNLNDALESRKVVERAKGVLM
                     DTYQLRESEAFHRIRKASMDSRKSMKEVAEAILLTHQMEMVSKGTEPGTENSST"
     gene            complement(1050..2324)
                     /locus_tag="VHR64_16410"
     CDS             complement(1050..2324)
                     /locus_tag="VHR64_16410"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX3304470.1"
                     /translation="MEERSRQPLRTEWGSRFLLSLVLAIGLWGWVTATGDPETTKRFP
                     SIPITVEGLRDGFVLVGEVGSADVSVTGPRSAIEDLVSTQVEALVDLDQITEPGTYPV
                     RVQVPKPDKTWSTTSRPRSINVIVEEESTVAFPVVPKPSGSLGSNQQVESVNPSTSEV
                     TVTGPNSLVQRVVQVELPIDIANRTTDFASVFTPVAVDKDGQPIGGVTLSPSSISATV
                     EITTRGKRVAVIAQIEGDPASGFEVVDRLVNPNTVLVDGPSDVLDQMITVNTDTVSIS
                     GAEGDVAQRVRIVGLPAGVTLLEPQSGMVDVVVQIRQRGVQQPLPEQPVSVINVEPGL
                     TATASPDTVILTVIGNEQELQNLTPSSLSVQVDARGLQPGTYQLQPTVLLPPNMEWKS
                     IEPTTITVTIIQEGAATPAATPQASPSASPTP"
     gene            complement(2337..3248)
                     /gene="cdaA"
                     /locus_tag="VHR64_16415"
     CDS             complement(2337..3248)
                     /gene="cdaA"
                     /locus_tag="VHR64_16415"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX3304471.1"
                     /translation="MSELTWLLSRLGEPRSLIDIAVVTGLLFWLLWVLQGTRATQLLR
                     GAAIFLISAILLSRLLDLRALGWLIDNMWPVVIVAVPIIFQPELRRALEQLGHTGTWL
                     RTSLPAGSDTGTMASTIDEVVRAAAQLSRLRYGALMVLERETGLQDYADRGVALDALV
                     SRQLLVTIFFPNSPLHDAAVLLRGDRIVAGSVVLPLTDNISPMSNLGTRHRAGIGVTE
                     DSDALAIIVSEETGQISVAHHGRLIRNLDQDRLRRVLRTLARIERVDREVEASGVRRA
                     IADRIAPFADRRAKQKRSETRPSASKS"
     gene            complement(3346..5799)
                     /locus_tag="VHR64_16420"
     CDS             complement(3346..5799)
                     /locus_tag="VHR64_16420"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX3304472.1"
                     /translation="MTVPLGNSHMVGGTNGFGVTRPIAEPQTVPFPQTSPEPPLATVR
                     LHASGQATAAISQHGAVTGAMTPQIDSTVPLGPNGATATMPLSDELQSLRAQIQQDPN
                     NADLVFELALALNEAGERNEANELLHRLIAIYEAQGDHEQATRIRSMVGGMGTAQIAD
                     NDAHTHVMGRNTTESLGRRTGTLSLRSNAAQRDGRVGFGKKNEERERPVFSVREVAFF
                     DHLPKTERLNSEAAGFYAKAEEERGRGRYRSALDQMQMAVAADPSVAALFLRLAELQL
                     KLGYRRQALDTVNALQRNEPVLRSDIPEWAFARVRLHAEPFDLAKVKRLVDGLVSDGH
                     GDIAAPYAARLVEHLSLDGRIDEAQSYSDRICALAPGDTRAALEAVVLALRYSGRGSA
                     IDRWEYALRNGADALVAKASMAAIIAADNEMDHWRALGDILPAYRKSGNHLIADAYRR
                     SAEALGWSPLQKSAASLFFLDGSDSGVRSALAIAAGDRQGSAIGRAAAAAALARILET
                     AGRGDEYLAAIRTTLGLFADQRIPTGVNWAGLLGYEPSIAEMSCELGQELTRAGDAAG
                     AVEVLKQGHAFDKRHMGLTQALAEAYARTNQLGSALTILDELAMSHRKGGRLDEMASV
                     LRQMSQLAPSNIKVKSRLVDAYLQRGFVAEARAELIQRADLEERAGKAREAIISLQRA
                     ADLSWNLGFPQETFNLYDRILAIDPEDVSNRSALVNLYLQVGRLSDAAEHQRAVVDLA
                     LKNGRKHEAIAALHQVIGLTPDDMTAYYQLGEALSSMGEYLQAEKVYRRIVLMNPDDA
                     VAQAKATAMAALKEQVLGQ"
     gene            complement(6170..6976)
                     /locus_tag="VHR64_16425"
     CDS             complement(6170..6976)
                     /locus_tag="VHR64_16425"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3304473.1"
                     /translation="MNEIQPNILRRASSDAEVYRPAETPVCPKCQGAGWLRLDVPLGH
                     PSFGRLFRCECQQRTEDRARVTEMRRLSQLDGLEGKSFAAFEADTADLTRALETARAY
                     AQSLSGWLVLSGRCGTGKTHLAAAIANETLEKGDQTVMFAVVPDLLDHLRATFDPARG
                     VDYDERFQAIRNTFLLVLDDLGTENATPWAREKLYQIVNHRYNERLPTVITTNQAPSA
                     IDERILSRMLDSTLSKRIQLDGEDFRRRGDPTYFRGSRGRPGTTTPRRRG"
     gene            complement(6985..7644)
                     /locus_tag="VHR64_16430"
     CDS             complement(6985..7644)
                     /locus_tag="VHR64_16430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEX3304474.1"
                     /translation="MPELTSSAPVDSTPLVSVPRALLDRLITTRASVEEHRIVLLLVL
                     LTRTGKADAAISEDAFATDSMVLDAGKVDGTPIAMPDWPFPALEQAVAHGTILRFVVE
                     SQAGSRNWLLLNTVENARLVARMAEDPGAVPEMFWIDESRPHVHVDKPTVFRLYEQNI
                     GPLTPMIADRLIKALETYPPDWIESALEEAVAYNRRNWRYIARILENWEADGPPGRTG
                     R"
     gene            complement(7659..>8665)
                     /gene="dnaB"
                     /locus_tag="VHR64_16435"
     CDS             complement(7659..>8665)
                     /gene="dnaB"
                     /locus_tag="VHR64_16435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX3304475.1"
                     /translation="RTATLRRLIDAGTKVVSIAFRDAVETQEALDEAEQAIFEVSQNR
                     VPRGFLPMREVLDGLFDQMSDDTLRAQGVPTGFVAVDRVLGGLQRSDLIVIAARPSVG
                     KTALALSMAYNAAVLHRKAVGFISLEMSAQQIVQRILASETGVNSHRLRMGQINDDEW
                     GAVGRAYGKLSEAPIFIDDGGSSTVNDVRTKARRLQAEYGLDMLVIDYLQLMQSRRSD
                     NRVQEISEISRGLKQVARELNIPVVALSQLSRAVESRAGHKPLLSDLRESGSIEQDAD
                     VVMLMYREDRYDPETARKGIADIDIAKHRNGSTATVHLRFFEATTRFADFEPYREEPL
                     "
BASE COUNT         1579 a         2667 c         2782 g         1637 t
ORIGIN      
        1 cgaacaagat ccaggtcatc aaggccgtcc gcgagctgac gagcctgggt ctcaaggagg
       61 ccaaggatct ggtcgaggcc gcaccgaagc cggtcaagga gggcgtctcg aaggacgagg
      121 ccaacgccgc caaggcgaag ctcgaagagg ccggcgccaa ggtcgaggtc aagtaactcg
      181 attctttgca ctgaatggcc cagcgtggtg gagagtcacc gcgccgggcc attttcattt
      241 cgcaccgtag tggttacttt tccgggcgac ccgagacacg gtggtatact ccgcacatcg
      301 gggcgtggcg cagcccggta gcgcaccggt ctggggtacc ggtggtcgga ggttcaaatc
      361 ctctcgcccc gaccaaccgg gtttggatct caagtgggaa gctgagtgat gcagagcaac
      421 acgcaatcgc gtgctccacg ccgcattctc attgcggacg atgaatcact gattcgactc
      481 gacctgcgcg agatgctgaa tcatctcggg tacgaggtcg tcggagaagc cggcgacggc
      541 aggcaggccg tcgagctcgc ccgcaagttg aagccagacc tggtcgtgat ggatatccgg
      601 atgccggagg tagatggcat ccaggcggcc ggaatcctga cctcggagcg gattgcgcca
      661 gtcgttctcc tgaccgcctt ctcggatacc gagctcgtcg agcaggcacg cgaggcgggc
      721 gtcgtcggct acgtcgtcaa gccgttccgc gaagccgagc tcatgccggt catcgaactc
      781 tcattcgctc gattcgaaga attcagggcg cttgagaagg aaatcggcaa tctcaatgac
      841 gcgctcgaat ctcgcaaggt cgtcgagcgc gccaagggcg ttctgatgga cacataccag
      901 ctccgcgagt cggaagcgtt ccatcgcatt cgcaaggcga gcatggattc ccgcaagtcc
      961 atgaaggaag tcgccgaggc cattctcctc acgcaccaga tggagatggt cagcaagggc
     1021 accgaacccg gcaccgaaaa cagctcaacc tagggagtcg ggctggccga cggcgacgcc
     1081 tgtggggtcg ctgctggtgt cgccgcgccc tcctgaatga ttgtgaccgt gatcgtcgtc
     1141 ggttcgatgc tcttccactc catgttcggt gggagtagga cagtcggctg taactggtaa
     1201 gtcccgggct gaagtccgcg ggcatcgacc tggaccgaga gcgacgacgg cgtcaggttc
     1261 tgcagctcct gctcgttgcc gatcacggtc aggatgaccg tatccggaga tgccgtggcg
     1321 gtcaggccag gttcaacgtt gatcacgctg accggttgct cgggcagggg ctgctggacg
     1381 ccgcgttgcc tgatctggac gacgacatcg accatcccgg actgcggctc aagcagcgtc
     1441 acaccggcgg gcagaccgac gatcctcacc cgctgggcca catccccttc agcaccgctg
     1501 atgctgacgg tatccgtgtt caccgtgatc atttggtcga gcacatcgct tggaccatcg
     1561 accagcacgg tattcggatt gacaagacga tcgactacct cgaatccgct cgccggatcg
     1621 ccttcgatct gggcgatgac cgcgacgcgt ttgccccgcg tggtgatctc gacggtcgcc
     1681 gagatcgacg acgggctaag ggtgacaccg ccgatcggtt gaccatcctt gtctacggca
     1741 acaggcgtga agacgctggc gaagtcggtc gtccggttcg cgatatcgat cggcagctcc
     1801 acctgcacca cccgctgtac gagcgaattt ggcccggtca ccgtcacttc ggacgtgctc
     1861 ggattgacag attcgacttg ctggttggag cccaggctgc ctgacggctt cggcacgacc
     1921 ggaaaggcaa cggtcgattc ttcttcgacg atgacgttga tggaccgggg gcgggacgtc
     1981 gttgaccagg tcttgtcagg tttcggcacc tgaacccgga ccggatacgt gcccggctcc
     2041 gtgatctgat cgagatcgac gagtgcttcg acttgagtcg ataccaggtc ctcaatcgcc
     2101 gagcgcggac cggtcacgct cacgtccgcg gaaccaactt cgccgaccag gacgaatcca
     2161 tccctgagtc cttcaaccgt gataggaata ctcgggaaac gcttggtcgt ttcgggatct
     2221 cccgtcgccg tgacccatcc ccacaaaccg atcgccagta cgagcgacaa caggaagcgc
     2281 gagccccatt ctgttcttag cggttgcctg gatcgctctt ccacacgtct gtccgtttaa
     2341 ctcttcgagg cggaggggcg agtctccgat cgcttctgct tggcgcggcg atcggcgaac
     2401 ggcgcaattc gatcggcgat cgcacgccgc acgcccgagg cttcgacctc acgatccacg
     2461 cgctcgatcc tggccagtgt ccgaagtacc ctgcgcaggc ggtcctgatc caggttgcgg
     2521 attagacggc cgtgatgggc gaccgagatt tggcccgtct cttccgaaac gatgatcgca
     2581 agggcatcgg agtcctctgt tacgccgata cctgcccggt gccgggtacc caggttgctc
     2641 atcggcgaaa tgttgtcggt aagcggcaac acaacgctgc cggcgacgat gcggtcaccg
     2701 cgaagcaata cagccgcgtc gtggagagga gaattcggga agaagatcgt gaccaacaat
     2761 tggcgcgaaa ccagtgcgtc gagcgcgacg ccgcgatcag catagtcctg gaggccggtc
     2821 tcgcgttcga gaaccattaa tgcgccatac ctgagacgcg agagctgcgc ggcggcccga
     2881 accacttcgt cgatcgtgct cgccatcgtt ccggtgtcgg agccggccgg cagcgacgtc
     2941 cgcagccagg ttccggtgtg gcccaattgt tccagcgcgc gccgcaactc cggctggaag
     3001 atgatcggca ccgcgacgat gacgaccggc cacatgttgt cgatcagcca gccgagggcc
     3061 cgcaggtcaa gcagcctgga gagcaggatc gcgctgatca aaaagatagc tgcgccgcgc
     3121 aggagttggg tcgcgcgcgt gccctgcagg acccagagca gccaaaacag caaccccgtg
     3181 acgacggcaa tgtcaataag agaccggggc tctccaagtc gtgacagcag ccaggtcaac
     3241 tctgacatcg gtgttttgat tcacattggg tggacgcaaa ggcgcgcgaa atcgacgaaa
     3301 cgatggtaac acggcgcccg catcgcaggg cgccgtgacc cgggactatt gccccagcac
     3361 ctgctccttg agagcagcca tcgcagtcgc cttcgcttgc gcgacggcat cgtctggatt
     3421 catgagaacg atgcgccggt agaccttctc ggcctgcagg tattcgccca tcgaggagag
     3481 cgcctcaccg agttggtaat acgccgtcat gtcgtccgga gtcaagccga tgacctggtg
     3541 cagtgcggcg attgcttcat gcttgcggcc gttcttgagc gccaggtcga ccacagcgcg
     3601 ctggtgctcc gctgcatcgc tcaggcgccc gacctgcaga tacaggttga cgagggcgct
     3661 gcggttcgag acgtcttccg gatcgatggc caggatgcga tcgtagaggt tgaacgtctc
     3721 ctgcgggaaa ccgaggttcc agctaaggtc ggcggcgcgc tggaggctga tgatcgcctc
     3781 cctggccttg cctgcccgct cttcgaggtc ggcccgctgg atcaattcgg ctcgggcttc
     3841 ggccacaaag ccacgctgca ggtatgcatc gaccaatcgt gacttgacct tgatgtttga
     3901 cggcgcgagc tgcgacatct gccggaggac agaggccatt tcgtcgaggc gaccgccctt
     3961 tcggtgcgac atcgcgagct catcgagaat tgtcagggcc gacccaagct ggttcgtacg
     4021 agcgtatgcc tcggcgagcg cctgggtaag gcccatgtgc ctcttgtcga aggcatggcc
     4081 ctgctttagg acttctaccg ctcccgcggc gtcgccggct cttgtcagct cctgcccgag
     4141 ctcgcaactc atctcagcga tgctcggctc atatccgagc aggcccgccc agttcacgcc
     4201 ggtcggaatc cgctgatcgg cgaacagacc gagcgtggtg cgaatggcgg ccaggtactc
     4261 gtcgccgcgg ccagccgtct cgaggatacg tgcgagcgct gctgccgccg ccgccctgcc
     4321 gatggccgaa ccctgtcgat cgcccgcggc tatcgcgagg gcagatcgga cgccggagtc
     4381 ggatccgtcg agaaagaaga ggctcgcggc cgacttctgc agcggggacc agcccagtgc
     4441 ctcggccgaa cgacgataag cgtccgcgat caggtgatta ccgctcttgc gatatgccgg
     4501 caggatgtcg ccgagtgcgc gccagtgatc catctcgttg tcagcagcga tgatcgcggc
     4561 catcgatgcc ttggcgacca gtgcatccgc tccattgcgg agggcgtact cccagcggtc
     4621 gatcgcgcta ccgcgccccg agtatcgcaa tgcgagcacg accgcttcga gggctgcgcg
     4681 ggtgtccccg ggagccaggg cacagatacg gtccgaatag gactgcgcct cgtcgatgcg
     4741 accgtcgagc gacaggtgct cgaccaggcg cgcggcatat ggtgcggcga tatcgccatg
     4801 accatccgaa accaggccat ccacgagccg cttcaccttg gcgagatcga acggctcggc
     4861 gtggaggcga acgcgggcga atgcccattc cgggatatcc gatcggagga ccggttcgtt
     4921 ccgttgcagc gcattgaccg tatcgagtgc ctggcgtcga tagcccagct ttagctggag
     4981 ctcggcaagg cgcaggaaca gcgccgcaac tgaaggatcc gcggcgacgg ccatctgcat
     5041 ctggtcaagc gcggagcgat atcggccgcg gccgcgctcc tcctcagcct tcgcgtaaaa
     5101 tccggccgcc tcggaattca accgctccgt tttcggcaag tgatcgaaga acgcgacctc
     5161 gcggacactg aagaccggtc gctctcgctc ctcgttcttc ttgccaaatc caactcgacc
     5221 atcacgctgg gcggcattgg aacgcaacga caacgtgccg gtgcgtcgcc ccagcgattc
     5281 ggtcgtgttg cgtcccatga cgtgggtatg agcatcgttg tcggcaatct gggcagttcc
     5341 catacctccg accattgaac gaatccgggt cgcctgttcg tggtcgccct gtgcctcgta
     5401 aatcgcgatc agacgatgga gcagctcgtt cgcttcgttc cgctctcccg cttcgttcag
     5461 cgccagggcg agctcgaaga cgagatcggc attgttcgga tcctgctgga tctgcgcgcg
     5521 caggctctgg agctcatcgg agagtggcat ggtcgcggtt gcgccgttcg gcccgagcgg
     5581 caccgtgctg tcgatctgcg gagtcattgc gccggtcacg gcaccatgct ggctgatggc
     5641 agcggtcgcc tggccgctgg cgtgcaatcg cacggtcgcc aacggtggct cgggacttgt
     5701 ctgcggaaac ggtacagtct gcggttccgc gataggtcga gtgacgccaa agccatttgt
     5761 cccgccgacc atgtggctgt tcccgagcgg aacggtcatc gatggattac tcgcgatggg
     5821 agcggtcatt tgtgaagttt ggaaggatgc agtcggcgcg ccaccgttga caggcgccag
     5881 gttcaccgtc gcatccggta ccgtggtatg tccagccata ggcgcggttt gggcggccat
     5941 tggcgcagtc caggctccga gacgacgctt gatgttatcc gattggtctg tctgggcccc
     6001 gactgtatgt tcgccgacgc gagacgtcgt gatgtcctga ctcaccgata ccctccgtgc
     6061 acagccttcc cattccgcac caggcggaaa ttcggctact tcgagtgtat ggggagggtc
     6121 atagccggag aataggactt aggcactgac cgtccggaaa cgacggttgt tagcctcgtc
     6181 gccgtggcgt ggtcgtcccg ggtcggccgc gactgccgcg aaagtaggtg ggatcaccgc
     6241 gccggcggaa atcttcgcca tcgagctgga ttcgcttgga gagcgtgctg tcgagcatcc
     6301 gcgaaaggat gcgctcgtca atcgcgctcg gggcctgatt cgtggtaatg acggttggca
     6361 gacgctcgtt gtagcggtga ttgacaatct ggtagagctt ctcccgtgcc catggcgttg
     6421 cgttctcggt gccgaggtcg tcgagtacga gcagaaacgt gttccggatc gcctggaacc
     6481 gctcgtcata atcaacgccg cgcgccgggt cgaacgtcgc ccgaagatga tccagcaagt
     6541 ccggcacgac agcgaacatg acagtctgat cgcccttctc gagcgtttcg ttggcaattg
     6601 ccgcagccag atgggttttc ccggtgccgc agcggcctga caggaccaac cagccgctga
     6661 gcgattgggc gtatgcgcgc gctgtctcca gtgctctcgt caaatcggcc gtgtcggctt
     6721 cgaatgcggc gaaggacttc ccctcaagtc catcgagctg cgacaggcgc cgcatctcgg
     6781 tcacccttgc tcgatcttcg gtgcgctgct ggcattcaca acgaaagagg cggccaaagc
     6841 tgggatggcc gagcggtaca tccagtcgca gccagcccgc gccctggcac ttggggcaga
     6901 ccggtgtttc agccggtcga tagacctcgg cgtcggatga cgcgcggcgg agaatattgg
     6961 gctggatttc attcacgggt cgcatcatct gcctgtcctg cccggcggac catccgcttc
     7021 ccaattctcg aggatgcggg cgatgtaacg ccagttgcga cgattgtacg caaccgcctc
     7081 ttcgagtgcc gattcgatcc agtcgggcgg gtaggtctcg agcgccttga tcagccgatc
     7141 cgcgatcatc ggcgtcagcg gaccgatgtt ctgctcgtag aggcggaata cggtcggttt
     7201 gtcgacgtgg acgtgtgggc gtgactcatc gatccagaac atctcaggaa ctgcccctgg
     7261 gtcctcggcc atgcgcgcaa cgagtcgcgc gttctcgacc gtgttgagca ggagccagtt
     7321 tcgggatcca gcctggctct cgacgacgaa tcgcaggatc gtaccgtggg cgaccgcttg
     7381 ctcaagcgcc ggaaagggcc aatcgggcat ggcgatcggc gtgccatcca ctttgccggc
     7441 atccagcacc atcgaatcgg ttgcgaaagc gtcttcggaa atcgcggcgt cggccttacc
     7501 ggtccgggtc agcagcacga gcagcagcac gatgcgatgt tcttcgaccg aggcgcgggt
     7561 cgtgatcaat cgatcgagca gggctcgggg gacagagacg aggggagtgg aatcaacagg
     7621 agcggacgac gtcagttcgg gcattcgagc aaccgtgcct acagcggctc ttcgcggtac
     7681 ggctcgaagt ccgcaaagcg ggtcgtggcc tcgaagaatc gcaagtgaac cgttgcggtg
     7741 ctgccgttac ggtgcttggc gatatcgatg tcggcaatgc ccttgcgcgc cgtttccggg
     7801 tcgtagcgat cctcccggta catcagcatg acgacatcgg cgtcctgctc gatcgatccc
     7861 gattcgcgca gatcggagag caatggcttg tgtccggcgc ggctctcgac ggcgcgggaa
     7921 agctgggaga gggcgacgac cgggatattc agctcccggg cgacctgctt cagcccgcgc
     7981 gaaatctcgg aaatctcctg tacgcggtta tctgagcggc gcgactgcat gagctgcagg
     8041 taatcgatga cgagcatgtc gaggccatat tcggcctgca gccgccgggc tttggtccgc
     8101 acgtcgttga cggtcgagct gccgccgtcg tcgatgaaga tcggtgcttc gctgagtttg
     8161 ccgtacgcgc gcccgaccgc gccccactcg tcgtcgttga tttggcccat gcgcagccgg
     8221 tgcgagttga cgccggtttc ggaggcgaga atgcgctgaa cgatctgctg ggccgacatc
     8281 tcgagcgaaa taaaaccaac cgccttcctg tgcaggactg cggcgttgta ggccattgaa
     8341 agcgccaggg cagtcttgcc gacgctgggc cgggccgcga ttacgatcag gtcggatcgc
     8401 tgcaggccgc cgagaacgcg atcgaccgcg acgaacccgg tcggcacgcc ctgggcgcga
     8461 agcgtgtcgt cgctcatctg gtcgaacaga ccgtcgagca cctcgcgcat tggcaggaaa
     8521 ccgcgcggga cccggttctg cgagacctcg aaaatggctt gctctgcctc gtccagcgct
     8581 tcctgggttt cgaccgcatc ccggaatgcg atgctgacga ctttggtgcc ggcgtcgatc
     8641 aggcggcgaa gcgtggcggt gcgct
//
LOCUS       DATAOZ010000658        12666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2624426, whole genome shotgun sequence.
ACCESSION   DATAOZ010000658 DATAOZ010000000
VERSION     DATAOZ010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12666
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2624426"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1744
                     /locus_tag="VHR64_16440"
     CDS             149..1744
                     /locus_tag="VHR64_16440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX3304476.1"
                     /translation="MPIDYDLLAERLATLKTNRRRFGIGAAGAVASLSGAAVLATKAL
                     GNESNHPEPTQPPLAAQDQPPTAVAGVSNAPNAAPTVIRSAPQAALPNGMALVSSPRL
                     PLFDLASQDVGALVSGQLPSWIEVGSALDLAVAPVAINGVTMDGLTAGQTFADYEELA
                     GFLNSPDGYGGVALVPADQVDARVNVLAVDGIDPVRNGQFDEPTMRIGFVGDIVPGRN
                     VGIKMREYNDYTHPFHRVAAILSSYDLTIANLEGDLSDDLPVPTEGATFDFVASTQMI
                     DGLAMAGIDAVSQANNHSTWNSAGWGTQGLTDTIDALDARSFGHFGGGRTLEEARAPY
                     TTTVAGKSVAILGIDGVTANVEAREPDATVNEAWLGGAKYAGATNDSPGTSPYDPDIF
                     LPDIETLVGQYDIVIPYFHFGREYVEVLPDWAVDGARSAIDAGATLVVTNHPHVIQGM
                     EFYAGKPILYSVGNFIFDQMFSVQTRQGLILELVLRGGKCVGLRTRGVEIEDFNQPRL
                     MNAGEQAAIMDRFWASTDRLANR"
     gene            complement(1784..2314)
                     /locus_tag="VHR64_16445"
     CDS             complement(1784..2314)
                     /locus_tag="VHR64_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX3304477.1"
                     /translation="MGDSTQTEVQIIARLPVERWQEYRELRLRALRTDPQAFGQSWAE
                     AVNHPEERWRQRLIDAEQGKSWLVFAERNGQLIGMSGAYQWPEDIEANRAMIIAVFVE
                     PEARGCGIGERLVQALMDVLTAAGLESAILAVNPVQSAAVRLYEKMGFRVTGSEINMM
                     GTGEECEETIMERPLS"
     gene            complement(2349..2915)
                     /locus_tag="VHR64_16450"
     CDS             complement(2349..2915)
                     /locus_tag="VHR64_16450"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX3304478.1"
                     /translation="MDFSLFHAVNHLAGHYDSIDDLVEFISSKGPIALVLMILAAWFW
                     PGSRVERDQRQWGGIAATIAASLALAINQGIIRVWDRPRPFEAHQVVLLLKPSADPSF
                     PSDHATFAFAVAVALFLAWRRLGIAALVVAVLISLSRVYVGEHYLGDVVAGAVIGSVM
                     AVAVFQLRTVAMPLIDPPMRLARRVRLA"
     gene            complement(3020..3982)
                     /locus_tag="VHR64_16455"
     CDS             complement(3020..3982)
                     /locus_tag="VHR64_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX3304479.1"
                     /translation="MDIVQFWPWALFLLFVGGMLAIDLGVLHRDAHEVSRKEALIWTG
                     FVVSSAILFNIGVFLFKGTDAGIEWTTGYLIEQSLSVDNVFVFLLLFGAFQVPAAYQH
                     RVLFWGILGALVMRAILIAFAGVLIHSLHFVIYLFGAFLIFTGIKFLRDKDSHVPDLE
                     GNRVVKLARRFFPVTEGYEGQRLFVKRNGVRFMTPLMLVLILIESTDLVFAVDSIPAI
                     YAVTDDPFIVFTSNIFAILGLRSLYFVLAGYLAGLKYLKPGLAGVLTFVGAKMLLVDV
                     YKIHPLVSLAVIIGILTVAFVASVRAKDRDQSVGIAGSVDANPA"
     gene            complement(4021..4455)
                     /locus_tag="VHR64_16460"
     CDS             complement(4021..4455)
                     /locus_tag="VHR64_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304480.1"
                     /translation="MLDEIFELFERDKNRRNGRPTGKRSLLDRVSGMLGDDDRRRDDD
                     RYDRRYDDDRSRYDERRRYEDDRRDDDVRRYEDDRYRDDRYARSRYDDDERPRYAEDR
                     YERRRDDDDFAERRGDDDDRYRSSEYRKPSKKRRLADLLDFD"
     gene            4749..6032
                     /locus_tag="VHR64_16465"
     CDS             4749..6032
                     /locus_tag="VHR64_16465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3304481.1"
                     /translation="MQVTTAPTLDSGTVGAIQRRIVLILAIAQVFGGVGVATGAAVGA
                     LLAADLSSDSFSGLASAASVTGAALIAIPVSRLMDQHGRRPGLMLAYAIGICGAFTTV
                     IGASLGWFPLALLGLIGIGGGTTATLQSRYAATDLATPERRGRALSTVVWATTLGSVL
                     GPNLASPMGRFAGHVGVPALAGPYLLTMCALLVSGVIIATFLRPDPLHVARDVRHIKE
                     GHSAMAPVERSMRTALETIAASPGARNGLAAIVLGHAVMVAVMSMTPLHLRHADEGLR
                     VIGIVISLHIAGMYAFSPLVGIAVDRIGRRPVITLGSLILLSAFLIAGTASGHQAPQL
                     SIGLCLLGLGWSCTMISGSTLLTDSVPIEQLPNVQGTADVMMGFGGASAGLLAGLVVG
                     IGSYAILAMVAACVILPLLFVTLLQPRVATVQSPA"
     gene            6043..7422
                     /locus_tag="VHR64_16470"
     CDS             6043..7422
                     /locus_tag="VHR64_16470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX3304482.1"
                     /translation="MSSQYDIYRAANRRLEALIDPNPAHDSSLREVQARAAQRLKAMA
                     AFLDYLGNPHRSAPVIHVGGTSGKGSTATAIAAILSASGRSTLLHTSPFLQVSTEKLQ
                     IDGRLIDAPLFAELTDEVIDAAGRYNQIHLTYGEAWVAIVALAMARLKPDVAVIEVGA
                     GGRFDLTNVVDSSVAVITTIGLDHTETLGPTIPEIAWHKAGIIKKANLVVAHGVEQPE
                     ARAEIDRQAAQVGVSSLIRIDPAALQIEQTDDGHPTWRDPVTGTRLKAGTPGLPQAQN
                     GALAVAAARAFVPDLDLAAIEQGLAQTRIAARFERMPGQGMVILDGAHNAQKVGAIIP
                     DLMRLPRPRVGVIGFLAAKRSDEMIDLLGPALDHVVVARPDVLGKPGREIAHAAESVR
                     ARVPVPVVAAQSPVDAVQIAEGIAGANGSVIVTGSLYLCGAVRERWYAGREIIEQKTQ
                     WPGTNPRPS"
     gene            complement(7426..7656)
                     /locus_tag="VHR64_16475"
     CDS             complement(7426..7656)
                     /locus_tag="VHR64_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304483.1"
                     /translation="MLAIILASVGSVAAATGFMLIGVSKSEFDENSLMTNVSWVLIVG
                     GVIMAVLGGLWYRSNEARLAEERSRSESLTRG"
     gene            7783..8634
                     /locus_tag="VHR64_16480"
     CDS             7783..8634
                     /locus_tag="VHR64_16480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX3304484.1"
                     /translation="MSDIVIVEELARHYGAVRAVDGISFAVAEGEIFGLLGHNGAGKT
                     TTIRMLTGRTRPTAGHASIAGYDVVEELDQIKPIINLVFEDANLYERLTGRDNLAVFA
                     RLYGVPVSRADELLEQLDLTDAAKRRVKTYSTGMKQRLIIARALINTPKVLFLDEPTR
                     GLDPASARELREMILRLAQQGTTVFLTTHYMNEADELCDRVAFLKEGRIVALDSPREL
                     KLKYGRRAARVLLDSREEREIDLTSPADAQALSDWMSHGRVMTLHSEEGTLEDVFINI
                     AGPAHIS"
     gene            8631..9725
                     /locus_tag="VHR64_16485"
     CDS             8631..9725
                     /locus_tag="VHR64_16485"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3304485.1"
                     /translation="MNRSLTRIFTLFTKDLKDAVRDARVLIALIVPLGIGVFYNYSFD
                     DSSIDEIKGTVAIAAADATQLPDLIQAAVPSNVHLTFETKPDQAAVEQAVSNEDADIG
                     LVVPAGFDQAVQQGAQPALTVIRSPKPSVPGDYVLSTLEPVLRQMAGQEFPVAINITD
                     AAEKPSETILDKIGLRTWSLTMAIVLMLALVSALAIPIVLAEEFEKKTIDALVLAMPY
                     REVIIAKALLGLFYIAVMVTVFVNLTQLDVYRWGLFLLGTGLTGVALLGFGLLLAGTL
                     KNANQLNTWSGVFLLPFLAPALIVGQPAPELLRQAAGLLPSGAGMKLLLNSVTEQQLF
                     AGNARFILILVLWAAAAYLLLLWQLKRRQA"
     gene            9722..10126
                     /locus_tag="VHR64_16490"
     CDS             9722..10126
                     /locus_tag="VHR64_16490"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX3304486.1"
                     /translation="MTIPHDPAPHPEYLRSTASIPTLIGRAFRRQCPVCGYHPIWKGW
                     MTLVDSCPNCGYVFRREGGYFLGAYALNLIVAEFIAMFVLVALLVWTAWEWWEIELVV
                     LPLAIGLPILFFPFSRGLWMALDLTFTPKNQR"
     gene            10350..12176
                     /locus_tag="VHR64_16495"
     CDS             10350..12176
                     /locus_tag="VHR64_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304487.1"
                     /translation="MRGVGEIREAALTGMQTERIRGLDSDVDNHGPGKREVDLYVRTY
                     TTLLQSSGAISVASLEPAHITAASSLHAGALEPEPDLNAFIYSVQRLPASIVDVRLIV
                     LGQQQKAFARAGFRNMSSWEIQSAPGRRRRWLFDGSETLAAYIASSSDLDDLIPTIVA
                     YQIEWNKMHRIIRDDDRLGDLLESAGEDGAILTIDDIAEIGERLLLSSVGWQRLQSVW
                     GSMLWRNLSLIAAGKKRHELMMVGGSFVGYTRSTRQWWAPVERLLGEMKLDQRPIYFV
                     SSNMHSFANVLTGTARRRERELKQFIRESTALGLADELEKIETGKLESSLDNLLYFAA
                     RPYFALPAQLEDRERRAEEEMERGVYHIDPRSAVDVGVQVIDLRKTDLSSIDPRLCQS
                     ANETLDLAASDAIIVNVNYPLGFAAYHLLSQVAMATEQIRGIYVLGKAATLNGRIGDV
                     MLSNVVFDEHSGNTYWFDNCFSYQDVAPFLLFGAALDNQKAVTVKGTYLQNQGYLDFY
                     YRENFTVVEMEAGPYLSAIYEDSRLERHPSEQAINLRGNVGHDIDLGIIHYASDTPYT
                     RAQTLGARGMSFYGMDSTYASTIAILRRIFQKTGTLKSREVE"
     gene            complement(12442..>12666)
                     /locus_tag="VHR64_16500"
     CDS             complement(12442..>12666)
                     /locus_tag="VHR64_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304488.1"
                     /translation="GFVTATFLLMTSLATGRNAPFRMIGGPELSSDPVRDVALKVMLI
                     SIGMNVLGRSLPRRQVMNYLREKVATSHAG"
BASE COUNT         2275 a         4460 c         3702 g         2229 t
ORIGIN      
        1 caacgtccaa cgtccaacgt ccaacgtcca acgtccaacg tccaacgtcc aacgtccaac
       61 gtcctccctc aaccctccac cctcaactct ccacgcggta cactcacggc catgatttcg
      121 ccgccaataa ccaggactgc tccgatccat gccgattgat tacgaccttc ttgcggagcg
      181 cctggcgacc ctgaagacca accgccgccg cttcggcatc ggcgcggccg gcgcggtcgc
      241 gtccctcagc ggcgcggcgg tcctcgccac caaggcactc ggcaacgaga gcaaccaccc
      301 tgagccgacc cagccgccgc ttgccgctca ggatcagcca ccgaccgcgg tcgccggcgt
      361 ctcgaacgct ccaaacgcgg ccccgacagt catccggtcc gcgccgcagg ccgctctgcc
      421 gaatgggatg gcgctcgtct cgtcgccccg ccttccgctc ttcgaccttg cctcgcagga
      481 tgtcggagcg ctcgttagcg gccagttgcc gtcctggatc gaggtcggct ccgcgctcga
      541 tctcgccgtc gcgccggtcg cgatcaacgg cgtgaccatg gatggcctca ccgccggcca
      601 gaccttcgcc gactacgagg aattggccgg cttcctgaac tcgcccgatg gctacggagg
      661 cgtcgcgctc gtcccggccg accaggtcga tgcacgcgtc aatgtcctcg cggtcgacgg
      721 catcgatccg gtccgcaacg gccagttcga tgagccgacg atgcggatcg gcttcgtggg
      781 cgacatcgtc cccggccgca acgtcggcat caagatgcgc gagtacaacg actacacgca
      841 ccccttccat agagtcgcgg cgatcctcag ctcgtacgac ctgaccatcg ccaacctcga
      901 gggcgacctc tcggacgacc tgccggtgcc gactgagggc gcgacgttcg atttcgtggc
      961 cagcacccag atgatcgacg gcctcgcgat ggccggaatc gacgcggtca gccaggccaa
     1021 caaccacagc acatggaaca gcgccggctg gggcacgcag ggcctgaccg acacgatcga
     1081 cgcgctcgac gcccgctcct tcggccattt cggcggcggc cgcacgctcg aggaggcgcg
     1141 cgctccctac accacgacag tggccggcaa atcggtcgcg atcctcggga tcgacggcgt
     1201 gaccgccaat gtcgaggcgc gcgagccgga cgcgaccgtc aacgaggcgt ggctcggcgg
     1261 cgcgaaatac gccggcgcga ccaacgattc gcccggaacc tccccctacg accccgacat
     1321 cttcctgccc gacatcgaga ccttggtcgg ccagtacgac atcgtcatcc cgtacttcca
     1381 cttcggccgg gagtacgtcg aggtgctgcc ggattgggcg gtcgacggcg cgcgctccgc
     1441 gatcgatgcc ggggcgacgc tggtcgtcac gaaccatccg cacgtcatcc agggcatgga
     1501 gttttacgcc ggcaagccga ttctctactc ggtcggcaac ttcatcttcg accagatgtt
     1561 cagtgtccag acccgccagg gcctgatcct cgagctggtc ctgcgcggcg gcaagtgcgt
     1621 cggcctccgc acccgtggtg tcgagatcga agacttcaat cagccccgcc tgatgaacgc
     1681 cggcgagcaa gccgcgatca tggaccgctt ctgggcctcc accgatcgcc tcgccaatcg
     1741 gtagagattc ttcgcacttc tggatctgct cgtagtttcc cgattatgag agaggccgct
     1801 ccatgatggt ctcttcgcat tcctcgccgg tgcccatcat gttgatttcg ctgccggtga
     1861 cgcggaagcc catcttctca tagaggcgca ccgctgcgga ttggaccggg ttgacggcaa
     1921 ggatcgcgct ctcgagcccg gccgctgtca gcacgtccat gagggcctgc accagccgct
     1981 caccgatgcc acacccgcgc gcttcaggct cgacgaagac cgcgatgatc atggcgcgat
     2041 tggcctcgat atcctccggc cactggtacg ccccggacat cccgatcaac tgcccgttcc
     2101 gctcggcgaa gaccagccag ctcttgccct gttcggcatc gatcagccgt tgtcgccagc
     2161 gctcctccgg atggttgacc gcctccgccc aggactgccc gaacgcctgc ggatccgtcc
     2221 tgagcgcgcg caatcgcagc tcgcgatact cctgccagcg ctcgactggc agccgcgcga
     2281 tgatctggac ttcagtttgg gtcgaatcgc ccataggacc ggcagaatac cagtccccag
     2341 cgactgggtc acgcgaggcg gacgcgccgc gccagccgca tcggcggatc gatcagcggc
     2401 atcgccaccg tccgcaactg gaacacggcc accgccataa ctgacccaat gaccgcgccg
     2461 gcgaccacat cgccgagata gtgctccccg acatagaccc gcgacaggct gatcaggacc
     2521 gcaacgacca gcgccgcgat cccaagccgc cgccacgcca ggaacagcgc aaccgccacg
     2581 gcgaaggcga acgtcgcgtg gtcgctcgga aatgacggat cggcagaagg cttgagcagg
     2641 agcacgacct ggtgcgcctc gaagggacgc ggccgatccc agacccggat gatcccctga
     2701 ttgatcgcca gagccagaga ggcggcgatc gtggccgcga tgccacccca ttgccgctga
     2761 tcccgctcga cccgggaacc gggccagaac cacgccgcca ggatcatcag gaccagcgcg
     2821 atcggcccct tgcttgaaat gaactcgacc agatcatcga ttgaatcgta atggccagcc
     2881 aggtgattga cggcatgaaa gagggagaag tccaaccagt cgatccttcc cattacgcag
     2941 gaaacgggca atcaaaacgc cacaccccat ggtcgagacg tggcgctttg attctacgtc
     3001 gtttgcccgc caaatggttc tatgccgggt tcgcgtcgac cgagccggcg atgcccaccg
     3061 attggtccct gtccttcgcg cgcaccgagg ccacgaaggc gacggtcagg atgccgatga
     3121 tcaccgccag gctgaccagc gggtgaatct tgtagacgtc caccagcagc atcttcgccc
     3181 cgacgaaggt cagcacgccc gccaggcccg gcttgaggta cttcagcccg gccaggtacc
     3241 cggccagtac gaagtagagc gaccgtaatc ccaagatcgc gaagatgttc gacgtgaaga
     3301 cgatgaacgg atcatccgtg accgcataga tcgccggaat cgagtcaacc gcgaagacga
     3361 ggtcggtgct ctcgatcagg atcaggacca gcatcagagg cgtcatgaac cgcacgccat
     3421 tccgcttcac gaacaggcgc tgcccctcgt acccctcggt caccgggaag aaccgccgcg
     3481 ccagtttcac gacgcgattg ccttcgaggt ccggcacatg gctgtccttg tcccgcagga
     3541 acttgatgcc cgtgaagatc aggaacgcgc cgaagaggta gatcacgaag tgcagggagt
     3601 gaatcagcac gcccgcaaag gcgatcagga tcgcccgcat taccagcgcg ccgaggatgc
     3661 cccagaagag cacccgatgc tgatatgccg ccggcacctg gaacgcgccg aagagcagca
     3721 ggaagacgaa gacgttgtcg acgctgagcg attgctcgat caggtagccg gtcgtccact
     3781 cgatccccgc gtcggtcccc ttgaacagga agacgccgat attgaacagg atcgccgatg
     3841 agacgacgaa accggtccag atcagcgcct ccttgcgcga cacctcatgg gcgtcgcgat
     3901 ggagcacgcc gaggtcgata gccagcatcc cgccgacgaa cagcagaaac agggcccacg
     3961 gccagaattg cacgatatcc acagcttgat ctcctcgttg actttctcag gttcaggtgc
     4021 ctagtcgaag tccagaagat cagccagccg ccgcttcttc gacggcttcc ggtactcgga
     4081 gctgcgatac cggtcatcgt cgtccccgcg tcgctcagcg aagtcatcat cgtcgcggcg
     4141 ccgctcatac cggtcttctg cgtaccgggg ccgctcgtca tcgtcatacc gcgaccgtgc
     4201 gtaccggtcg tctcgatatc ggtcgtcctc gtagcgccgg acatcgtcgt cccgtcgatc
     4261 gtcctcatat cgccgccgtt cgtcataccg ggagcgatcg tcgtcgtatc tgcggtcgta
     4321 tcggtcgtcg tcccggcggc gatcgtcatc accgagcatt ccggagaccc gatccagtag
     4381 cgatcgcttc ccggttggcc ggccatttcg cctgttcttg tcgcgttcga acagctcaaa
     4441 gatctcgtcg agcatcgatg tggctccttc tgatgatgtg ccggaaacaa aaagacccgg
     4501 ccatccgaaa tcggataagc cagggtctga ccggacgtca tcgaaggtat cgatgcccgc
     4561 gttcctgacc ggcgccacgg cgtaatgacg accaggaacc gcctcgcgac gcgagacgtg
     4621 ggttactccc cctgtttgac taaaccctac ggattcgacc cgccggtgtc aaggcacggt
     4681 gaagtaagat gcagcgccgc acggggccaa ccggcgtgcg agaatgaccg gcgcgtatga
     4741 gagaacccat gcaagtgact acggcaccga ccctcgattc tggcactgtc ggcgcgatcc
     4801 agcgccggat cgtgctcatt ctcgccatcg cgcaggtttt cggcggcgtt ggcgtcgcca
     4861 ccggcgccgc ggtcggcgcg ctgctggccg ccgacctatc gtcggactcg ttcagcggtc
     4921 tggcgtccgc ggcctccgtc accggcgcgg cgctgatcgc gatcccggtc tcgcgactca
     4981 tggaccagca cggccgtcgt cccggcctga tgctcgccta tgcgatcggc atctgcggcg
     5041 ccttcacgac cgtgatcggc gccagtctcg gttggttccc gctcgccctg ctcggcctga
     5101 tcgggatcgg cgggggcacg actgccaccc tccagtcccg ctacgccgca accgacctgg
     5161 caacgcccga gcgccgtggc cgtgcgctct ccacagttgt ctgggcgacc acgctcggtt
     5221 ccgtactcgg tcccaacctt gcctcgccga tgggtcggtt cgctggccac gtcggcgttc
     5281 ccgcgctggc cggaccctac ctgctgacca tgtgcgcgct acttgtctca ggggtgatca
     5341 tcgccacgtt ccttcgtccc gacccccttc acgtcgcgcg cgacgtgcgc catatcaagg
     5401 aggggcacag cgcgatggcg ccggtcgaac gctcgatgcg tacagcactc gagacgatcg
     5461 ctgcgtcgcc tggagcgcgc aatggtctgg ccgcgatcgt gctcggtcac gccgtgatgg
     5521 tcgccgtcat gtcgatgacc ccactccatc tgcgccatgc agatgaaggg ctccgcgtga
     5581 tcggcatcgt gatcagcctc catatcgccg gcatgtacgc cttctcgccc ctggtcggca
     5641 tcgcggtcga ccggattggc cgccggccgg tgatcacatt gggcagcctg atcctcctaa
     5701 gcgctttcct gatcgccgga accgccagcg ggcaccaggc gccccagctt tccatcggcc
     5761 tctgcctgct cggtcttggc tggtcctgca cgatgatctc cggctccacg ctcctgactg
     5821 attccgtgcc gatcgagcag ttgccgaacg tccagggaac ggccgacgtg atgatgggct
     5881 tcggcggagc gtcggccggg ttactggccg gtctcgtcgt cggaatcggc tcgtacgcga
     5941 tcctggcgat ggtcgccgcc tgcgtcatcc tgccgctgct cttcgtgacc ctgcttcagc
     6001 cgcgggttgc gaccgtccag agcccggcct gacgaacaga cgatgagcag ccagtacgac
     6061 atctaccgcg ctgccaaccg gcggctcgaa gccctgatcg accccaatcc ggctcacgac
     6121 tcatcgctcc gtgaagtcca ggcccgcgcc gcccagcggc tcaaggcgat ggcggccttc
     6181 ctggattacc tcggtaatcc ccaccgctcc gctccggtga ttcacgtcgg cggcacctcg
     6241 ggcaagggct cgaccgcgac tgccatcgcc gcgattctgt ccgcctcggg ccggtcgacg
     6301 ctcctccaca cttcgccttt cctgcaggtc tccaccgaga agctgcaaat cgacggccgg
     6361 ttgatcgatg cgcctctctt tgccgagttg acggacgagg tgatcgatgc cgccgggcgc
     6421 tacaatcaga tccacctcac ctacggtgag gcctgggtgg cgatcgtagc cctggccatg
     6481 gcccgtctca agcccgatgt cgcggtgatc gaggtcggcg ccggcggtcg cttcgacctc
     6541 accaacgtcg tcgactcctc ggtcgccgtc atcacaacga tcggtctcga tcacaccgaa
     6601 accctcggcc cgaccatccc ggaaatcgcc tggcacaagg ccggcatcat caagaaggcc
     6661 aacctggtgg tcgcacacgg cgtggagcaa cccgaggccc gggccgagat tgatcggcag
     6721 gcggcacagg tcggcgtctc ctcgcttatt cggattgacc cggccgcgct ccaaatcgag
     6781 cagaccgacg acggtcaccc gacatggcgc gatccggtca ccggaactcg actgaaagcg
     6841 ggcaccccgg gtctgccaca ggcccagaac ggcgcgctcg ccgtcgcagc cgcccgcgcc
     6901 ttcgtcccgg acctggatct cgccgcgatc gagcagggtc tggcccagac gcgcatcgcc
     6961 gcccgcttcg agcgcatgcc cgggcagggc atggtcatcc tcgacggcgc ccataacgcc
     7021 cagaaggtcg gcgcgatcat ccccgatctg atgcgcttgc cgcggccccg ggtcggcgtg
     7081 atcggctttc tcgccgccaa gcgctccgac gagatgatcg acctgctcgg cccggcgctc
     7141 gatcacgtgg tggtcgcgcg gcccgatgtt ctcggcaagc cgggacgcga aatcgcgcac
     7201 gcggccgaat ccgtgcgcgc gcgtgtcccc gtgccggttg tcgcggcgca aagcccggtt
     7261 gatgcggtcc agattgctga agggattgcc ggagcgaacg gctcggtgat tgtgaccggt
     7321 tcgctctacc tctgcggagc tgtgcgggag cgctggtacg ccggccggga gatcatcgag
     7381 cagaaaactc aatggccggg gacgaatccc cggccatcct gacgcctatc cgcgcgtgag
     7441 cgattcgctc ctgctgcgct cttccgcgag gcgcgcctca ttcgagcggt accagagacc
     7501 gccgagcacg gccatgatca cgccgccgac gatcagtacc cacgacacgt tcgtcatcag
     7561 gctgttctcg tcgaattccg acttcgagac gccgatcaac atgaagccgg tcgccgccgc
     7621 caccgagcca actgaggcca gaataattgc cagcatattc ccgcagctcc tcgggttgtg
     7681 atgcccgttt gtcccgacca atccctggcg ggtagtgtac tgtctcacgt ggcaggattt
     7741 gcgcacgcct gccacccacg tgcgacacga ccagcgagca gtatgtccga tatcgtcatt
     7801 gtggaagagc ttgccaggca ctacggcgcc gttcgcgccg tggacgggat ctcgttcgcc
     7861 gttgccgagg gtgagatctt cggcttgctc ggccacaacg gcgccggaaa gaccaccacg
     7921 atccggatgc tgaccggccg cacccggcct accgccggcc atgcctcgat cgccggttac
     7981 gacgtggtcg aagagctcga ccagatcaag ccgatcatca acctcgtctt cgaggacgcc
     8041 aatctctacg agcggctgac cgggagggat aacctcgccg tctttgcccg gctctacggt
     8101 gtgccggtct cccgggccga cgagctgctc gaacagctcg acctgaccga tgccgccaag
     8161 cgccgcgtca aaacctactc gaccggcatg aagcagcgcc tcatcatcgc ccgggccctg
     8221 atcaacacgc cgaaggtgct gttcctcgat gagccaacgc gcggtctcga ccccgcctcg
     8281 gcccgcgagc tgcgcgagat gatcctgagg ctcgcccagc aaggcacgac ggttttcctg
     8341 acgacccact acatgaacga ggccgacgag ctctgcgatc gcgtcgcctt cctcaaggag
     8401 ggccgcatcg tcgcgctcga cagtccgcgc gagctcaaac tgaagtacgg ccgccgcgct
     8461 gcccgtgtcc tgctcgacag ccgcgaggag cgcgagatcg acctcacctc gcccgccgac
     8521 gcccaggcat tgagcgactg gatgtcgcac ggccgcgtca tgaccctgca ctccgaggaa
     8581 ggcacgctcg aggatgtctt cattaatatc gcgggaccgg cccatatctc atgaaccgat
     8641 cactgacccg catcttcacg ctcttcacca aggacctcaa ggacgcggtt cgcgacgccc
     8701 gcgttttgat cgccctgatc gtgccgctcg gcatcggggt cttctacaac tactccttcg
     8761 acgattcctc gattgacgaa atcaagggca ccgtcgcgat cgccgccgcg gacgccacac
     8821 agcttcccga cctgatccag gccgcggttc cgtcaaatgt tcacctcacc ttcgagacga
     8881 aacccgatca ggccgcggtc gagcaagccg tcagtaacga ggacgctgat atcggtctcg
     8941 tcgtcccggc agggttcgat caggcggtcc agcagggagc gcaaccggcc ctgaccgtca
     9001 tccggtcccc caagccatcc gtgcccggcg actatgtcct gtctaccctg gaacccgtcc
     9061 tgcgtcagat ggcgggccag gagttcccgg tcgccatcaa catcaccgat gcagccgaga
     9121 aaccctccga aaccatcctc gataagatcg gcctgcggac ctggtcgctc accatggcga
     9181 tcgtcttgat gctggccctc gtctcggcac tcgcgattcc gatcgtgctt gcagaggagt
     9241 tcgagaagaa gacgatcgat gcgctagtcc tggcgatgcc atatcgggaa gtgatcatcg
     9301 ccaaggcgct tctcggtctc ttctacatcg ctgtcatggt cacggttttc gttaacctca
     9361 cccagctcga tgtatatcgc tggggtctgt tcctgctcgg gacgggactc accggcgtgg
     9421 ccctgctcgg cttcggcctc ctcctggccg gcacgctcaa gaacgcgaac cagctcaaca
     9481 cctggtccgg cgtcttcctg ttgccgttcc ttgctccggc cctgatcgtc ggacaaccgg
     9541 cgccggagct gcttcgccag gctgccggcc tgctcccaag cggcgcggga atgaagctcc
     9601 tcctcaattc ggtcaccgag caacagcttt tcgctggtaa tgcgcgcttc atcctgatac
     9661 ttgtgttatg ggccgcggcc gcttatctcc ttttgctctg gcaattgaaa cggaggcagg
     9721 catgacaatt ccgcacgacc cagcgccgca tccggaatat ctccgatcga ccgcctcgat
     9781 cccgaccctg atcggtcgcg cattcaggcg ccagtgcccg gtctgtggct accacccgat
     9841 ctggaagggc tggatgacac tggtggactc ttgcccgaat tgcgggtacg tcttccggcg
     9901 cgagggcggc tacttcctcg gcgcgtacgc gctcaacctg atcgtcgccg agttcatcgc
     9961 catgttcgtc ctggtggcac tcctggtctg gaccgcctgg gaatggtggg aaatcgagct
    10021 ggtcgtcttg ccgctcgcga tcggcttgcc gatcctcttc ttcccgttct cgcgcggcct
    10081 ctggatggcc ctcgatctca ccttcacccc caaaaaccag cgctaatgcg accgcgctgg
    10141 tcactgcccg ggcgtaaaca cccgggctga aacctgaagc cggcagaatt gagcgcgtgc
    10201 aacatgtggt gcgggtgtca cacaatgagg tcgcattgcc atgcgacgct gccgcgcttc
    10261 gctttccatc taccatccac catcaaccaa ccaccccttt tcgttccctc cacccttcac
    10321 caaacctata cttcgcggaa cctcacctgg tgcgaggagt cggagaaatt cgggaggcgg
    10381 cactgaccgg tatgcagact gagcggattc gcggcctcga tagcgacgtc gacaaccacg
    10441 gccccggcaa gcgcgaggtc gacctctatg tccgcaccta caccaccctg ctgcagagct
    10501 ccggcgcgat ctcggtcgcc agcctggagc ccgcccacat cacggccgcg tcatccctcc
    10561 acgccggtgc gctcgaacca gaaccggacc tcaacgcctt catctactcg gtgcagcggc
    10621 tcccggcctc gatcgtcgac gttcgcctga tcgtcctcgg ccagcagcag aaagcattcg
    10681 ctcgcgctgg cttccggaac atgtcgagct gggagatcca gagcgccccc ggccgtcgtc
    10741 ggcgctggct cttcgacggt tccgagacgc tggcggccta catcgcctcc tcgtccgatc
    10801 tcgacgacct gatcccgacg atcgtggcct accagatcga gtggaacaag atgcaccgca
    10861 tcatccggga cgacgaccgt ctcggcgatt tgctggagag cgccggcgaa gatggcgcga
    10921 ttctgacgat cgacgacatc gccgagatcg gcgagcgcct gctgctctcg agcgtcggct
    10981 ggcagcgcct gcagagcgtc tgggggtcca tgctctggcg caacctctcg ctgatcgccg
    11041 ccggcaagaa gcgccacgag ctgatgatgg tcggcggcag cttcgtcggc tacacgcgct
    11101 ccacccgcca gtggtgggcg ccggtcgagc ggctgctggg agagatgaaa ctcgatcagc
    11161 gcccgatcta cttcgtctcc tcgaatatgc acagcttcgc caacgtcctg accggtaccg
    11221 cccgccgccg cgagcgtgag ctgaagcagt tcatccgcga gtcgaccgca ctcggcctag
    11281 ccgatgagct cgagaagatc gagaccggca agctggagtc atcgctcgac aacctgctct
    11341 acttcgcggc ccgcccctat ttcgccctgc cggcccagct cgaggaccgc gagcggcgcg
    11401 ccgaggagga gatggagcgc ggcgtctacc acatcgatcc ccgctcggcc gtcgatgtcg
    11461 gcgtccaggt catcgacctc cgcaagaccg acctctcctc gatcgatccg cgcctctgcc
    11521 agtccgcaaa cgaaacgctg gacctggccg cttcggacgc gatcatcgtc aacgtcaatt
    11581 acccgctcgg cttcgccgcc taccatctgc tgtcccaggt cgcgatggcg accgagcaga
    11641 ttcgcggcat ctatgtcctc ggcaaggccg ccacgctcaa cggccggatc ggcgacgtca
    11701 tgctctcgaa cgtcgtcttc gacgagcact ccggcaacac ctactggttc gacaactgct
    11761 tctcgtacca ggatgtcgcc ccgttcctcc tcttcggcgc cgcgctcgac aaccagaagg
    11821 cggtcaccgt caagggcacc tacctccaga accagggcta cctcgatttc tattaccggg
    11881 agaacttcac cgtcgtcgag atggaagccg gtccatacct gagcgcgatc tacgaggaca
    11941 gccgcctcga acgccacccc agcgagcagg ccatcaacct gcgcggcaac gtcggccacg
    12001 acatcgacct cggcataatc cactatgcct cagacacgcc ctacacccgc gcccaaaccc
    12061 tcggcgcgcg cggcatgtcc ttctacggca tggactccac ctacgccagc accatagcca
    12121 tcctgcggag aatcttccag aagacaggca cgctgaagag tcgggaagtc gagtagtcga
    12181 gaagagggcg cgatcgcgac caccaatccc gccggccgca agtcagcgaa cgtaagctga
    12241 ttgccaatgg agtaggcgta cgggttatac cggtcgtccc tgcctcctcg atctggacgc
    12301 tatctcggct caggaagctc gccgtgcggg acgcgaggta ccagatccgt ccgggccaag
    12361 cggtgcaacg tcaagagcgg ccgcagcggt gcctatctgt gcgcctaatc cgccgatctt
    12421 ttccatgact atgaaccatt gttaacccgc gtggctcgtc gccactttct cgcggagata
    12481 attcattacc tgtctccggg gtagtgagcg accaagcacg ttcataccaa tcgaaatcag
    12541 cattactttc agcgccacat ctctgactgg atcggacgac aattcaggac cgccgatcat
    12601 ccggaatggt gcgttacggc cggtggccag cgaagtcatc agcaggaatg tagcggttac
    12661 gaaacc
//
LOCUS       DATAOZ010000659        10638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7886893, whole genome shotgun sequence.
ACCESSION   DATAOZ010000659 DATAOZ010000000
VERSION     DATAOZ010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7886893"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VHR64_16505"
     CDS             complement(<1..1078)
                     /locus_tag="VHR64_16505"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX3304489.1"
                     /translation="MPNRLANEVSPYLQQHKDNPVDWYPWGGEALAKAKAEDKPLLVS
                     IGYSACHWCHVMAHESFEDPTTAELMNQHYVCIKVDREERPDIDSLYMTAVQAMSGQG
                     GWPLNVFTTPDGIPFYGGTYWPPDDRMGMPSFRRVLDAVAETWATKRDEIVQQGDQIR
                     DMLAHRPGGSPDGGDVELASIERAAQHLGGQFDPLNGGFGGAPKFPQAPVIEFLLRAQ
                     HLLNDHQPLDMALETLEKMARGGIYDQVGGGFHRYAVDNIWLVPHFEKMLYDNAQLAR
                     MYLDAYRISGRDLFRRTAEETLDYVLREMAAESGGFFAAQDADSEGEEGKFYVWTLEE
                     IRSVLSEDEAPIAESYFGVREGGNF"
     gene            1205..2083
                     /gene="proC"
                     /locus_tag="VHR64_16510"
     CDS             1205..2083
                     /gene="proC"
                     /locus_tag="VHR64_16510"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX3304490.1"
                     /translation="MDDRQNATETQNAGITCRISFVGAGVMAESMIAGLLAKQVVRPD
                     QINASHPRADRLAKLGERFGISTTADNRQAAQAADVVFLTVKPQVLWEVMPQLAGSLT
                     PRQVVVSIVAGANIETLESGLLHNMIARVMPNTPAQVGAGMMVWAATPQVTEEQRQRV
                     RTVLSALGEELFVDEEKYVDMATALSGTGPTYVFLMMEALIDAGVHMGFPRRIAEQIV
                     LQTVGGSVEFARASHKHMAELRNMVTSPGGTSAEAIYQMEKGGLRTVFSRAVYAAYLR
                     TQSLAEEQSKHRPRNP"
     gene            2205..3509
                     /locus_tag="VHR64_16515"
     CDS             2205..3509
                     /locus_tag="VHR64_16515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX3304491.1"
                     /translation="MAVLAQTTRSTKARPNSRRRKLFAASLIVLIAVSAVGGVYAWRM
                     VNAIVNAERTAVYPLPPRDSEVALQVSPTSTPEVDPTTAPAGQGDAPAPTATVVNVSA
                     SNSQANPAPTASVAALPTGDDPSRISVVQDLIGASVGSGDPGKSQVWGGKSAITLMVL
                     GVDKRPDGGDQNADVIIIAHVDLVNRKVAAVSIPRDLLVDIPGVGQDKINSAYNYGVK
                     ADPTSKVAGVAMMRDTVESVFGVYIDGYIMVDFNGFTNVIDAMGGVVVDVPSQIVDDQ
                     YPTENYGTEVVRFMPGRQLMDGDRALKYVRTRHQDSDDGRRERQLQVLRALFEQAKSF
                     DSLTNGFEIVTALGGSVQTSFYLDQQLTLAQLGYSMDDGDIQLSSLTAPLIWGGYLDN
                     GAWVYFSDPVAVRDWVTKSLSTDFTSVATPTSGGAIPIATPS"
     gene            complement(3510..4406)
                     /locus_tag="VHR64_16520"
     CDS             complement(3510..4406)
                     /locus_tag="VHR64_16520"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX3304492.1"
                     /translation="MIDIAFLGTGAMMPTAQRWLSSALLRIDSQLILLDCGEGTQIPW
                     RSLGWGFKRLSLICFSHWHADHVAGLPGILHALAVAERTEPLAIIGPKGTRDIVTSLR
                     ELAPVLPYDVVCADLANGQQWQQGNVRVDVCAGNHQVPVLVYRLSIARRPAFQVDKAQ
                     SGGVPREAWSRLADGVDVDGWKAEDYQGPPRRGLAVGFMTDTRPTQAALSLLQGVDLL
                     ISEGTYGDDADEGNAIQNKHLTFREAATFASEAGVRRLVLTHFSPKMERPRDYLSNAQ
                     SAFAATELADPGQVITLNFPDQ"
     gene            4424..5608
                     /locus_tag="VHR64_16525"
     CDS             4424..5608
                     /locus_tag="VHR64_16525"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX3304493.1"
                     /translation="MAVRSSLDIEALRAETPGCENVIHFNHAGSSLMPQSVVDAVVSH
                     TVREGEIGGYEAEDEAAQRIGAVYDHTATLLGADRSEIAMIENATRAWDMVFYAIPFQ
                     PGDRILTAMAEYHSNVIAFYQMRKRGVQVEVVPNDEHGQIDVDALAGMMDDRVRLVAI
                     SHMPTNGGLVQPAEEIGAVVAKWPAFYLLDACQSAGHKPLDVRAIGCDMLSASARKYL
                     RGPRGQGFLYVNSAVIDQLEPPMLDGHAATWSSRDSYELMPDARRFENWERSFANILG
                     MGEAITLANSLGLDAIWDRIQQQATTLRTRLAEIPGVSVTDVGEVQSGIVTFTYDGID
                     PDEIQKRLHAQRINVTTSSVASTRFDMEARGLEKVVRSSVHYLTTDEEIDQLVMTLGA
                     MR"
     gene            complement(5605..6840)
                     /locus_tag="VHR64_16530"
     CDS             complement(5605..6840)
                     /locus_tag="VHR64_16530"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX3304494.1"
                     /translation="MNALFNRLSLGLSGNLRVLLFTIFLWELGFGLYFNNMLTIYMRG
                     VGLTEAKVGTILTIAGLFRIGLMLPAGSIMDHVGRKPVVVGAAFISIPGSLAYIFADG
                     WVLLLVASICMSVNALGFPAMSAIIADSNAEDPLDAFRKLYTVGPAIAFIIGPLIGGQ
                     IAELISQRAVFVACAIVFMGAFLLALRLTEPPLHNHGNRRGGYVDIVRHRPIRLMVIY
                     GFVMVFVLGFGTTFLPNLVTDKYGFDDQQRGLAFSFGAVGTLILSVAMSKTKRITHIR
                     GVALGVLSVSGICVAALVLSNPYLLIPAFMMRGGFMMAWSLLTPLASDITPKHLQERT
                     FAGMEFSTGVGNTLAPIAAGVAYELNRSAPFLIAAIIMPVLAAIAIWLERRVVEPEVE
                     RRKRVDRLNAPPEPVATAA"
     gene            6934..7317
                     /locus_tag="VHR64_16535"
     CDS             6934..7317
                     /locus_tag="VHR64_16535"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX3304495.1"
                     /translation="MPRLRDPQSKDCDKVRNRVEGRGEPDVLKPPTPEEREQLASAGM
                     AWGMGCSIVVSILISILGGLLIDQAADTTPLFTLIGVGLGLFLAGYQLWELAQLSSKA
                     EKVGPVARQVARVQNRGKNRRTRDR"
     gene            7336..8268
                     /gene="atpB"
                     /locus_tag="VHR64_16540"
     CDS             7336..8268
                     /gene="atpB"
                     /locus_tag="VHR64_16540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX3304496.1"
                     /translation="MNIHVEIAAETLFHIGPIPVTNSMLTMFLVMAGILLVGWLIARD
                     LKEVPGRGQGLIEIIVGFILGIVEGTAGRKAARGLMPLVGGMFIFILFANYSGLLPGV
                     GTIGLWREPEHETTTEAPADSTHEEGAVLYQVASVNPVSAPTTAAAAEEEHDKVLVPF
                     FRAPNADINMTLAMALVTFVTVQIYGIRAHGVRGRIKHMASPAFLLPIEVIGEFSRII
                     SLSARLFGNIFAGEVLMGVMVAMANAIKIALIPLGLPAIFLLLEVLFGSIQALVFALL
                     TAIYITLATAGHDDHEEEHVEGHAIPQVAGSHGD"
     gene            8475..8681
                     /gene="atpE"
                     /locus_tag="VHR64_16545"
     CDS             8475..8681
                     /gene="atpE"
                     /locus_tag="VHR64_16545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX3304497.1"
                     /translation="MGAALAIGLGAIGPGLGIGNAVRGAMEALGRNPEAAGEIRSTMI
                     IGAALAEAVAIYAFVIAVIIAFVI"
     gene            8771..9274
                     /gene="atpF"
                     /locus_tag="VHR64_16550"
     CDS             8771..9274
                     /gene="atpF"
                     /locus_tag="VHR64_16550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF017242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX3304498.1"
                     /translation="MGDLGIDGWKLTVQLVAFLAFIFLLWKYALGPIVKMIDSRTEKI
                     EESMKSAEKMQADLKATAARNEEALHEARREAQQIVVNAREASEAMLNKAREDAGKQA
                     DEYLARAQASLRSETEQARVQLRQELADLAVTAAGKIVRKELDPATQASLIEETLAEA
                     TASNGRG"
     gene            9297..9830
                     /locus_tag="VHR64_16555"
     CDS             9297..9830
                     /locus_tag="VHR64_16555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX3304499.1"
                     /translation="MASGAAKRYTEAVFSIAQDKGSFDQWQRDLDALAGLMSDNGAAL
                     VLNSPKISRAQKLSLIQEALRGAQPEALNLATLLLDRNRLGIAPEMANLFRDAALAEL
                     GIVVAKVTTAEPIDRQAEAAIRQRLSSLVGKQVELRSEVDPSIIGGVVARIGDQLIDG
                     SVSNQLRRLRDRLEAGA"
     gene            9892..>10638
                     /locus_tag="VHR64_16560"
     CDS             9892..>10638
                     /locus_tag="VHR64_16560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922182.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX3304500.1"
                     /translation="MVLRATEIGDILRAQIAGFEKELTVSNVGTVVEVGDGIARVYGL
                     SQVMASELVEFTKTGVLGLAFNLEEDSVGVIIMGEYTEIEEGDEVRATGRIASVPVGE
                     ALVGRVVNALGQPIDGKGPIQTDKTRPIERIAPGVILRQDVDTALQTGIKAIDSMIPI
                     GRGQRELIIGDRQTGKTAVVLDTIINQKGQDLICIYVAIGQKDSSVAKIVATLEEHGA
                     MEHSIVVVAGASSPATLQYLAPFAGCAMGEY"
BASE COUNT         2066 a         3304 c         3389 g         1879 t
ORIGIN      
        1 cgaagtttcc accttcgcga acaccgaagt acgactccgc gatcggggcc tcgtcctcgg
       61 aaagcacgga tcgaatttct tcgagagtcc agacgtagaa cttaccctct tcaccctcgc
      121 tatcggcatc ctgcgcggcg aagaacccgc cggactccgc cgccatctcg cgcagcacgt
      181 agtcgagcgt ctcctcggcc gtgcgccgga acaaatcgcg cccggagatc cgataggcgt
      241 cgagatacat gcgcgcgagc tgggcattgt catagagcat cttctcgaag tgcggcacga
      301 gccagatatt gtcgaccgcg taccggtgaa agccgccgcc gacctggtcg tagatcccgc
      361 cccgggccat cttctcgagc gtttcgagtg ccatatcaag cggctgatgg tcgttcaata
      421 agtgctgggc acggagcagg aactcgatca ccggtgcctg cgggaacttc ggcgcgccgc
      481 cgaatccgcc gttgagcgga tcgaactgcc cgccgaggtg ctgcgcggct cgctcgatcg
      541 atgcgagctc gacatcgcca ccgtcaggac tgccgcccgg tcgatgtgcc agcatgtccc
      601 ggatctgatc accctgctgg acaatctcgt cgcgcttcgt cgcccaggtt tcggcgaccg
      661 catcgagcac gcgccggaac gacggcatgc ccatccggtc gtccggcggc caatacgtcc
      721 cgccgtagaa gggaatgccg tctggtgtcg tgaagacgtt gagcggccat ccgccctgcc
      781 cgctcatggc ctggaccgcg gtcatgtaga gcgaatcgat gtcgggccgc tcttcgcggt
      841 cgaccttgat gcagacgtag tgctggttca tcagctcggc ggtcgtcgga tcctcgaacg
      901 actcatgagc catgacgtgg caccaatgac acgccgagta gccgatgctg accagcagcg
      961 gtttgtcctc cgctttcgcc ttcgccagtg cctccccgcc ccacggatac cagtcgaccg
     1021 ggttgtcctt gtgctgctgg aggtatgggc tgacttcgtt ggcgagtcga ttgggcacgg
     1081 ggcgtctcct ggaatcgggt cggagcgaag tatcagtcca gtctacaagc cggaatccct
     1141 gcacaacccc gacgatgctg cgactacaat gccctgcgct taatcgaggt gacgctaggg
     1201 catcatggac gaccgacaga acgcaactga gacgcagaac gccgggatta cctgccggat
     1261 cagtttcgtc ggcgccggtg tgatggccga gtcgatgatc gccggcctgc tcgcgaagca
     1321 ggttgtccgg ccggaccaga tcaacgccag tcacccgcgc gcggaccgcc tggcgaaact
     1381 gggggagcgg ttcggcattt caacgaccgc tgacaatcgc caggcagccc aggccgcgga
     1441 tgtcgttttc ctgacggtga agccgcaggt gctttgggaa gtcatgccgc agctcgccgg
     1501 atcgctcacg ccccggcagg tggtggtctc gatcgtcgcc ggagccaaca tcgaaacgct
     1561 cgaatccggc ttgttgcaca acatgattgc ccgggtcatg cccaatactc cggcgcaggt
     1621 cggagccggg atgatggtct gggccgcgac gccgcaggtc accgaggagc agcgccagag
     1681 ggtgcggaca gtcctgtcgg cactcggcga ggagctcttc gtcgacgagg agaagtacgt
     1741 cgatatggcg accgcactca gcggaaccgg cccgacctac gtctttttga tgatggaagc
     1801 gctcatcgac gccggagtcc acatgggatt cccgcgccgg atcgccgagc agatcgtgct
     1861 gcaaaccgtc ggaggttcag tcgagttcgc gcgagcgtcg cataagcaca tggcagagct
     1921 gcgcaatatg gttacatcgc caggtgggac gtccgccgaa gcgatctatc aaatggaaaa
     1981 aggtgggttg cggaccgtct tttcgcgggc cgtttatgcc gcgtacttgc gaacccaaag
     2041 cctggcggaa gagcagtcaa agcaccgtcc tcgtaatccg taactcgaaa ccagatgagg
     2101 cggcgtgccg ttagcacagt agactagtcc ggttgggtat gcccaggcat tctcgtactc
     2161 gacatttggc ccgtgacgtt attccttttt agccgtgaga gagcatggcc gtactagccc
     2221 agacgacgcg ctccaccaag gcgcgcccga attcgagacg ccggaagctg ttcgccgcgt
     2281 cgctcatcgt gctgattgct gtgagcgcgg tcggtggcgt ctatgcgtgg cgcatggtca
     2341 acgcgatcgt gaacgccgag cggaccgcgg tctatccgct tcctccgcgc gatagcgagg
     2401 ttgcacttca ggtcagcccg accagcactc cggaggttga cccgacgacc gctccagcag
     2461 gacaaggcga tgctccggct ccaaccgcga cggtcgtcaa tgtcagtgcg agcaattcgc
     2521 aggccaatcc cgctccgacg gcatccgttg ccgcgctgcc gaccggagat gatccatccc
     2581 ggatcagcgt cgtccaggac ctcatcggcg cgtctgtagg ctccggcgat ccgggcaaat
     2641 cccaagtctg gggtggaaag tcggcgatca ccctgatggt gcttggggtc gacaaacggc
     2701 ccgacggagg cgatcagaac gccgacgtga tcatcatcgc ccatgtcgat ctggtcaacc
     2761 gcaaagtcgc ggccgtcagc atcccgcgcg atctcctcgt cgatatcccc ggagttggcc
     2821 aggacaagat caacagtgcc tacaactacg gcgtcaaggc cgacccgacg agcaaggtcg
     2881 ccggcgttgc catgatgcgc gacaccgtcg agtccgtctt cggggtctac attgacggct
     2941 acatcatggt cgatttcaac ggatttacga atgttatcga tgccatgggc ggcgtggtgg
     3001 tcgacgtgcc ctcgcaaatc gtcgacgacc agtacccgac agaaaattac ggcactgaag
     3061 tcgtccggtt catgcccgga cgccagctca tggacggcga tcgcgcgctc aagtacgtgc
     3121 gaacccgtca tcaggacagc gacgacggtc ggcgagagcg gcagctccag gtgctgcggg
     3181 cgttattcga gcaggccaag tcgttcgact cgctcacgaa cgggttcgag atcgtcacgg
     3241 ccctgggcgg atcggtgcag accagcttct atctggatca gcagttaacg ctcgcccagc
     3301 tcggatattc aatggacgac ggggatatcc agctctccag cctgaccgcg cccctgatct
     3361 ggggcggata cttggacaac ggcgcgtggg tctacttctc cgatcccgtt gccgttcgcg
     3421 actgggtgac gaagtcgctt tcaaccgact tcaccagcgt tgccacgccg acctctggtg
     3481 gcgcgattcc aatcgcgacg ccctcctgat cactggtcgg gaaaattgag cgtgatgacc
     3541 tggcccggat cggccaactc tgttgcggca aaggccgact gcgcattgga aaggtaatcc
     3601 cgaggccgct ccatcttcgg gctgaagtgg gtcagcacca gccgccgcac acctgcttcg
     3661 ctcgcgaacg tggccgcctc cctgaatgtc agatgcttgt tctggatcgc attcccctcg
     3721 tcggcatcgt caccatacgt gccttcggag atcagcagat ccacgccttg caggagcgag
     3781 agcgcggctt gcgtaggccg cgtgtcagtc atgaatccga ctgccaaccc ccgccgcggc
     3841 ggaccctggt aatcctccgc cttccacccg tcgacgtcca cgccgtccgc cagccggctc
     3901 caggcctcgc gcggcacgcc accagactgt gccttatcga cctgaaaggc cggccgtcgc
     3961 gcgatcgaca gccggtagac cagaaccggt acctggtgat tgcccgcaca gacatcgacc
     4021 cgcacgttgc cctgttgcca ctgttggcca ttcgccagat cggcgcagac cacgtcatag
     4081 ggcaggaccg gcgcgagctc gcggaggctc gtcacgatat cccgcgtccc cttcggcccg
     4141 ataatggcga gcggctcggt ccgttcggcc acggccaggg cgtgcaagat tccggggaga
     4201 ccggcgacgt ggtccgcatg ccagtgcgag aagcaaatca ggctcagccg cttgaaaccc
     4261 cagccgagcg atcgccaggg aatctgtgtt ccctctccgc aatcgagcaa gatcaactgg
     4321 ctgtctatcc gtagcagggc gcttgacagc cagcgctggg ccgtgggcat catggcgccg
     4381 gtgccaagaa aggcaatgtc aatcatcagg tgtgaggtcc cacatggcgg ttcggtcgtc
     4441 cctcgacatt gaagcacttc gcgcggaaac gccgggttgc gagaacgtca ttcacttcaa
     4501 tcatgccggc tcatcgctga tgccgcagtc ggtcgttgat gccgttgtat cgcacaccgt
     4561 tcgcgagggc gagattggcg gctacgaggc ggaggacgaa gcggcccaga gaatcggtgc
     4621 cgtctacgac cataccgcaa cgctgctcgg cgcagaccgg agcgagatcg ccatgatcga
     4681 gaacgccacc cgcgcctggg acatggtctt ctacgcgatt cccttccagc ccggcgaccg
     4741 catcctgacc gccatggccg agtaccacag caacgtcatc gccttctatc agatgcgtaa
     4801 gcgcggcgtg caggtcgagg tcgtgcccaa cgacgagcac ggtcagatcg atgtcgacgc
     4861 gcttgccggg atgatggatg accgggtccg gctagttgcg atctcgcaca tgccgaccaa
     4921 cggcggcctc gttcagccgg ccgaagagat cggcgcggtc gtggcgaaat ggccggcatt
     4981 ctacctgctc gatgcctgcc agagcgcggg tcacaagccg ctcgacgtgc gcgcgattgg
     5041 ctgcgacatg ctctcggcca gcgcccgcaa gtacctccgc ggtccccgag gccagggatt
     5101 cctctatgtc aattcggcgg tcattgacca gctcgagccg ccgatgctgg acggccatgc
     5161 cgcgacctgg tcgagccggg acagctatga gctgatgccc gatgcccggc ggttcgagaa
     5221 ctgggagcgg tcattcgcca acatcctcgg catgggcgag gcgatcacgc tcgcgaattc
     5281 cctcggtctc gacgcgatct gggaccgtat ccagcagcag gcaacgacgt tgcgaacgcg
     5341 gttggcagag atcccgggcg tgtccgtcac cgacgtcggc gaggtccaga gcgggatcgt
     5401 cacgttcacc tacgacggca tcgatccaga cgagatccag aagcggcttc acgcccagcg
     5461 catcaacgtg acgacatcat cggtggcgag cacgcgcttc gacatggaag cgcgcgggct
     5521 ggagaaggtc gtgcgctcat cggtccacta cctgaccacc gacgaggaaa tcgatcagtt
     5581 ggtgatgacg ctcggcgcga tgcgctaggc cgcggtcgcg accggctccg gtggggcgtt
     5641 gagccgatcc acccgcttgc gacgctcgac ctccggctcg acgacgcgcc gctccagcca
     5701 gatcgcaatc gccgccagga ccggcatgat gatcgccgcg atcaggaacg gcgccgatcg
     5761 gttgagttcg taggcgacac cggcggcgat cggcgccagc gtgttgccga cgccggtcga
     5821 gaattccatg ccggcgaagg tccgctcttg caggtgcttg ggtgtgatgt cgcttgccag
     5881 tggcgtcagc agcgaccacg ccatcatgaa cccgccgcgc atcatgaagg ccgggatcag
     5941 caggtacgga ttactgagca ccagggcggc gacgcagatg ccgctgacgg aaagcacgcc
     6001 gagggcgacg ccacggatgt gcgtaatccg cttcgtcttc gacatagcga ccgagaggat
     6061 gagcgtgcca acagcgccga acgagaacgc cagtcctcgc tgctggtcgt cgaagccgta
     6121 cttgtcggtc acaaggttcg gcaggaatgt cgtgccgaaa ccgagcacga agaccatcac
     6181 gaatccgtag atgaccataa gcctgatcgg ccggtggcga acgatgtcca catagccccc
     6241 gcgccgattg ccgtggttgt gcaacggtgg ctcggtcaga cgcagcgcaa gcaggaacgc
     6301 ccccatgaag acgatcgcgc aggcgacgaa gacggcccgc tgcgatatca actcggcgat
     6361 ctggccaccg atcagcggcc caatgatgaa tgcaatcgcg gggccgaccg tgtagagctt
     6421 gcggaaggcg tcgagcggat cctcggcatt cgaatcggcg atgatggccg acatggccgg
     6481 aaatccgagc gcattgaccg acatgcagat cgacgccacc aggagcagca cccagccatc
     6541 ggcgaagatg taggcgagcg acccgggaat cgagatgaac gccgcgccga ccacgaccgg
     6601 cttacggccg acgtggtcca taatcgagcc ggccggaagc atcaggccga tccgaaaaag
     6661 gccggcgatg gtcaaaatcg tgccgacctt ggcctcggtc agaccgacac ccctcatata
     6721 aatggtcaac atgttgttga agtagagccc gaacccgagc tcccacagga aaatggtgaa
     6781 gagaaggacg cggagattgc cggacaggcc gagtgacagc cgattgaaga gcgcgttcat
     6841 gagcctgtca catcacgtgg ccgggagcgg tcgcattcga tatcgatttg gcaggatacc
     6901 acccgggcag gctcgccgcg aacacggagt agaatgcccc gattgcgcga cccgcagtcg
     6961 aaagattgtg ataaagttcg caatcgggtt gagggtcgtg gggaaccgga tgtgttgaag
     7021 ccaccgacgc ccgaagaacg agagcaactc gcgtcagccg gcatggcttg gggcatgggt
     7081 tgctcgattg ttgtctcaat ccttatcagt attttgggcg gtttgctcat cgaccaggct
     7141 gcggacacga ccccgctgtt cactctgatc ggtgtcgggc tcggcttgtt tctcgccggt
     7201 tatcaactgt gggagcttgc gcagttgagt tcgaaggcag aaaaggtcgg gcctgttgcg
     7261 cgccaggtgg cacgtgttca gaaccgcggc aagaaccgtc ggacgagaga tcggtaggag
     7321 agcaggaagc gtcggatgaa tattcacgtt gaaattgcgg ccgaaacgct cttccacatc
     7381 ggccccatcc ccgtgaccaa ctcaatgctc acaatgttcc tggtcatggc cggaatattg
     7441 ctcgttggct ggttgatcgc ccgcgatctc aaggaggtcc cgggccgcgg tcagggactc
     7501 atcgagatca tcgtcggctt tatcctgggc atcgtcgaag gcaccgccgg gcgcaaggca
     7561 gcgcgcggcc tgatgccgct cgtcggcggt atgttcatct tcatcctctt tgccaactac
     7621 tctggactgc ttccgggagt cggcaccatc ggcctctggc gagagcccga gcatgagacg
     7681 accaccgagg caccggccga ttcgacccac gaggagggcg cagtccttta ccaggttgca
     7741 tcggtcaatc cggtcagtgc gccgacaaca gccgccgcag ccgaagagga gcacgacaag
     7801 gtgctcgtgc cgttcttccg agcgccgaac gccgacatca acatgacgtt ggcgatggcc
     7861 ctggtcacgt tcgtgacggt ccagatctac ggtattcggg cacatggggt ccgcggccgc
     7921 atcaagcata tggccagccc cgcattcctg ttgccgatcg aggtgatcgg cgagttctcc
     7981 cgaatcatct cgctctccgc ccgtctcttc ggcaacatct ttgccggcga ggtgctgatg
     8041 ggcgtgatgg tggcgatggc caatgcgatc aagatcgcgc tgatcccgct tggtctgccg
     8101 gcaatcttcc tgctccttga agtgctcttt ggatcgatcc aggcgcttgt cttcgccttg
     8161 ctgaccgcga tctacatcac gcttgccacg gcgggtcacg acgatcacga agaggagcat
     8221 gtcgaggggc acgccattcc ccaggttgcg gggagccacg gcgactagat tttgggtgca
     8281 acccggcggc gcgaatcgcg agccggaact gatatgaagg atcgagcagt cgatccgcac
     8341 cagtgagtaa cggagggcga caagggtcgt ccgaggggaa ggaagcaatt ccggcgttcg
     8401 ctgtgagttg cacaaggagg aacgacgtgt ttggcggatt gactggcacg gacggcgtag
     8461 acgcagccaa ggcgattggc gctgcactcg cgatcggcct gggcgcaatc gggccgggcc
     8521 tcggtatcgg taatgcggtt cgcggcgcaa tggaagcgct cggtcgcaac ccggaggctg
     8581 ccggcgagat ccggtccacg atgatcatcg gcgccgcgct cgccgaggca gtcgcgattt
     8641 acgccttcgt gattgcggtc atcattgcgt tcgtcatcta gtcatcagaa cgagcggcca
     8701 gtaacggtcg ctcccgagtg tgccgggcac gggctaaatc ggcccacggc acaagcgagg
     8761 actcatcgca atgggtgatc tcggaattga tggctggaag ctgacagttc agcttgttgc
     8821 attcctcgcc ttcatttttc tcctctggaa gtacgcgctg ggaccaatcg tcaagatgat
     8881 tgattcccgc acggaaaaga ttgaagagag catgaagtcg gccgagaaga tgcaggctga
     8941 cctgaaggcg accgcggccc gcaatgagga ggcgctgcac gaggctcgtc gcgaggctca
     9001 gcagatcgtc gtcaatgcgc gggaggcgtc cgaggccatg ctcaacaaag cccgggaaga
     9061 tgccgggaag caggccgacg aatacctcgc ccgcgcgcag gcgtcgctcc ggtccgagac
     9121 cgagcaggcc cgggtccagc tccgacagga gctcgccgac ctggccgtga ccgctgcggg
     9181 caagatcgtc cgcaaggagc ttgatccggc cacgcaggca tcgttgatcg aagagacgct
     9241 cgccgaggca acggcctcca acgggcgcgg atagcgccac caggagaagc agtgtcatgg
     9301 cgagtggagc agccaagcga tataccgagg cggtcttcag catcgcccag gacaagggca
     9361 gcttcgatca atggcagcgc gatctcgatg cgctggctgg tctgatgtcg gacaacggcg
     9421 ccgctctggt gctgaacagc ccgaagatca gccgcgcgca gaagttgtcg ctgattcagg
     9481 aagcgcttcg cggggcgcag cccgaggcgc tcaatctggc aacgctgttg ctggatcgca
     9541 atcgcctcgg gatcgccccg gaaatggcca acctcttccg cgatgccgcg ctcgccgagc
     9601 ttgggattgt cgtcgccaag gtaacgaccg cggagccgat cgaccgacag gccgaggcgg
     9661 ccatccggca acgcctgagc tcgctggtcg ggaagcaggt cgagcttcga tccgaggtcg
     9721 acccctcgat catcggcggc gtcgtcgccc ggattggtga tcagttgatc gacggcagcg
     9781 tttccaacca gttgcgccgc ttgcgcgacc ggttggaagc cggagcatag cgtgacagtt
     9841 ttggtagcta cgagtcctct tgtcgagggg tgtagccagg cagggtgaac aatggttcta
     9901 cgtgcaactg agattggcga catcctgcga gcgcagatcg ccggtttcga gaaagaattg
     9961 acggtcagca atgtcggcac cgtcgtcgaa gtcggagacg gcatcgcgcg ggtctacggc
    10021 ctgtcccagg tgatggcgag cgagctcgtc gagttcacga agaccggcgt cctgggcctc
    10081 gccttcaacc tcgaagagga ctccgtcggt gtcatcatca tgggcgagta caccgaaatc
    10141 gaagaaggcg acgaggtccg cgcgacgggc cggatcgcct cggttccggt cggagaggcg
    10201 ctcgtcggcc gcgtggtaaa cgcgctcggc cagccgatcg acggcaaggg cccgattcag
    10261 accgacaaga cccgtccgat cgagcggatc gcgcccggcg tgatcctccg ccaggacgtc
    10321 gataccgcgc ttcagaccgg tatcaaagcg atcgactcca tgatcccgat cggtcggggt
    10381 caacgcgagc tgatcatcgg tgaccggcag actggtaaga ccgccgtcgt gctggacacc
    10441 atcatcaacc agaagggcca ggacctgatc tgcatctacg tcgcgatcgg acagaaggac
    10501 tcgtcggtcg cgaagatcgt cgcgacgctc gaggagcacg gcgcgatgga gcactcgatc
    10561 gtcgtcgtcg ccggcgcgtc gagcccggcg accctgcagt acctggcgcc attcgcgggc
    10621 tgcgcgatgg gcgagtac
//
LOCUS       DATAOZ010000660         7808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_9201095, whole genome shotgun sequence.
ACCESSION   DATAOZ010000660 DATAOZ010000000
VERSION     DATAOZ010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9201095"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="VHR64_16565"
     CDS             <1..1520
                     /locus_tag="VHR64_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3304501.1"
                     /translation="QGDPTTLVIGLNGSPTDLDPHSQYDYRSVIPIRGPYEGLIILKD
                     SETDQYDGVLAESWEANEDKSVWTFHLREGVTFQDGSPCDAEAVRASYERLLTMQKGA
                     YNVVARFVPDPSHITAPDAKTVVFDLGKPQPLFESAMGATYGTQVVNVKVAKEHEEDG
                     DFGNTWMMTFAEGTGTGPYKITDFQPGDTVTLEKYDGYWAGWEGDHFDTIIVRTVNES
                     QTIRQLLEAGEVDITDRIALLPETVKDLESNPDLKVDRKTTTEVVYMTMTEYGPLESA
                     EARQAMCYAFPYAEVIQGAYAGYAKQPRGAVAEATRGFDPATFQYTTDLDKAKELLAT
                     AGVAEGTSLSLMVAGSTTIAELFQANLAEIGIKLDIQQVDTNLFTGTFYGDSPVEERP
                     AFMIWSWWPDYNDAWNHLDPQVSCDPHGSANAGMYCNERVDELLKASHDAANPEDYQT
                     AMSEIQQILSQDDPSAIYYAEPEWTTTMRKDIQGFFLNPINLGTYNLYKLHRGTA"
     gene            complement(1623..2774)
                     /locus_tag="VHR64_16570"
     CDS             complement(1623..2774)
                     /locus_tag="VHR64_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304502.1"
                     /translation="MKPRQYAGLAILLIVAVIVAAAEFWPTPEYRGAGAPAPEPDPIT
                     VTGIIGGKIAFLQDPEVTTLLKQKYGLTVDFKRVGSIEMINECQSGLDYCWPSSQTSG
                     EIIKNRLGTAVLANEIIFNSPLVLYTWAPIADALVKQGIVEKSGDTYYIVDLPRLINM
                     ISGGTPWSDIELPQLYGPVRVLTSDPTQSNTGNSFAGLLANTLNDGVVVDQTSVQAVT
                     PELQAFFGRLGLLPDTTTLLFEQFLSLGIGACPIIAAYESNMIEYNLAHPSQDAQDYV
                     RQNVRTLYPKPTVWTSQPLLALTPGGQRLMEALRDPQIQRIGWENHGFRTAVPGVINN
                     LAALGVTGVPQSIDSVIPMPGPAAMDIIIQSLSTPAEATPVAIKPDDLM"
     gene            complement(2771..3415)
                     /locus_tag="VHR64_16575"
     CDS             complement(2771..3415)
                     /locus_tag="VHR64_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304503.1"
                     /translation="MARSTSTYQRAVILGLQQDLVRGLIAGLLLLLVLALGASLWLAL
                     PISMIAYGGMRLMAGSTNHTPSTDSVNHRPRTGRDALALCQTLRPSITMLSFGLDDGR
                     PAAQITRVLCRTDQLIAAIEEDKQFDAAPMLLDLMETMIELLTPYAKAIRRGLDGYDV
                     HESLLRDLAILERALDLLWEKVNQETLASLSAISEMIEFNLEGIATLRQDGDRR"
     gene            complement(3425..5083)
                     /locus_tag="VHR64_16580"
     CDS             complement(3425..5083)
                     /locus_tag="VHR64_16580"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3304504.1"
                     /translation="MTWPICWVPPDVDEDVRHPLRGMSFILGFLLVMIMLAACVDRPG
                     PAYDATFRVLSGAENQPLEPIIQSFARDQKAKIEVSYAGSVDIMRELANGTNSRYDAV
                     WPASSIWILLGDSTNAVQFSSSIMRSPVVLGVKQSVAERLGWAGADVTVADVLAAAEA
                     DDFTFMMANAAQSDSGAAAYLGFLYAFAGQPDVLTSADLRNPAVQEKVKRILGTVSRS
                     AGNSGSLKDLFLSEYDSYDGMINYESTIIETNQALTAGGREPLYALYLRDGTSIADFP
                     LGEIAKGDKKKDALFQKLQAHLQSDDIQHQLLGLGRRTGIGVNPDPVNVDAAVFNPDW
                     GIDTQRILTPIRVPGAAVVQEALSLYQSALRKPSFTVFALDFSGSMNENGGADQLIAA
                     MHLLLDRQSASQYLLDTGPQDITVVIPFDDKILDTWTVTGNDPAQLRQLDEQIRAVKG
                     GGGTDIYSPVIRGLEIMKEHGIDGYFASVVLLTDGVSANGKTYGDLHSVISRESVAAV
                     PVFAITFGDASMDQLQPIADLTHGIVFDGRANLAATFRTVRGFT"
     gene            complement(5403..6950)
                     /locus_tag="VHR64_16585"
     CDS             complement(5403..6950)
                     /locus_tag="VHR64_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304505.1"
                     /translation="MLTSFSGRTRTLQLLAAILIPLLLIVPGQFVHPAAAQVSGNSYT
                     SPQFGFSMSWDDTWFVADETSNEIFDRVIVTNGLTYCTLLGGPDSSTTPQAALAGFIG
                     AVRLSSGVSDMAPMHADSGELIRGGDDQHAFAAFTYTLTLDDGREILLATYIEARIIV
                     PGESVVAFIAETPASAFDTQQPEFAKLLSTVRPGIDSPRSDPPLVDGEPAPVFVSGPW
                     RVAVATTAVRPTFPDLGLRKKTDKAWLVAVLDVTNWSDQDAVLSAHDFTIGTDPLQKP
                     ARIARSSMPAIADKLGTTPFAEDLTLEIAAGQTARVAVAFVIPAGAESPLLTVGSESL
                     PLADVIASDLRADALPAASGPPNLTEGEIVSATDGNTLRVMLDGKERSSRIRLLGVNP
                     PTDGSCFANAAESTLDDLAGATVLIESDPAITDGSVPSRYVWLVNDDGTRTLLNQRLI
                     AEGSAYAAILPAGARFTAWFDATAKTADLAGTGLWAGCASTEATASIGIATTPAAIPA
                     NREKATK"
     gene            complement(7100..>7808)
                     /locus_tag="VHR64_16590"
     CDS             complement(7100..>7808)
                     /locus_tag="VHR64_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3304506.1"
                     /translation="TAREVRGAYDECRRLQARIATLCGTMSDIRTRELTQQIVIHIDQ
                     IMEVIAEDNHPEICLKLIEPLTVTDTFLTNYAKLLRRGHDPAEMKERVRANLIMLDHG
                     FGEIWQELIQETVVDLGKLSDVIEETIEQLPVPADVATLPPELPESVGQSHPPHVAAL
                     VASLTPRQLAILRILTTGRTDQEIADMLFISKRTVTTHLTEIYDKIRVRNRAEAVAFA
                     VRWGLAPPDEPELTPVA"
BASE COUNT         1336 a         2214 c         2875 g         1383 t
ORIGIN      
        1 cgcagggcga tccgacgacg ctggtgatcg ggctgaacgg ctcgccgacg gatcttgatc
       61 cccattcaca gtacgactat cgctcggtga ttccgatccg cgggccgtat gaggggctga
      121 tcatcctcaa ggacagcgag acggaccagt atgacggcgt gctggcggag tcgtgggagg
      181 cgaacgagga caagagcgtc tggactttcc acctgcgcga gggcgtgacc ttccaggacg
      241 gctcgccgtg cgatgcggag gcggtgcggg cctcgtatga gcggttgttg acgatgcaga
      301 agggcgccta caacgtggtg gcccggttcg tgccggatcc gtcccacatc acggcgccgg
      361 acgcaaagac cgtcgtgttc gatctcggca agccgcagcc gctgttcgag tcggcgatgg
      421 gcgccaccta tggcacgcaa gtggtcaacg tcaaggtggc caaggagcac gaggaggacg
      481 gcgacttcgg caacacctgg atgatgacct tcgccgaagg caccgggacc gggccgtaca
      541 agatcaccga tttccagccg ggcgatacgg tcacgctcga gaagtacgac gggtactggg
      601 ccggctggga aggcgaccac ttcgacacga tcatcgtccg cacggtcaac gagtcgcaga
      661 cgatccggca gctgctggag gcgggtgagg tcgacatcac ggaccggatc gcgctcctgc
      721 cggagacggt caaggacctg gagtcgaacc cggacctgaa ggtcgatcgc aagacgacga
      781 ccgaagtcgt ctacatgacg atgaccgaat acgggccgct ggaatcggcc gaggcgcggc
      841 aggcgatgtg ctacgcattc ccgtacgccg aggtgatcca gggcgcgtat gccgggtacg
      901 cgaagcagcc gcgcggcgcg gtggccgaag cgacgcgtgg gttcgacccg gcgacgttcc
      961 agtacacgac cgacctggac aaggccaagg agctgttagc gacggccggg gttgcggagg
     1021 ggacgtcgct cagcctgatg gtcgccggct cgacgacgat cgcggagctg ttccaggcca
     1081 acctggccga gatcgggatc aagctggata ttcagcaggt cgacacgaac ctcttcaccg
     1141 gcaccttcta cggggactcg ccggtggagg agcggccggc cttcatgatc tggagctggt
     1201 ggccggatta caacgatgcc tggaatcacc tcgatccgca ggtctcgtgc gatccgcatg
     1261 gctcggccaa tgccgggatg tactgcaacg agcgggtgga tgagctgctg aaggcgtcgc
     1321 acgacgcggc caatcccgag gattaccaga ccgcgatgag cgagatccag cagatcctct
     1381 cgcaggacga tccgtcggcg atctactacg cggagccgga gtggacgacg acgatgcgga
     1441 aagacatcca gggtttcttc ctgaacccga tcaacctggg cacgtacaac ttgtacaagc
     1501 tgcaccgggg gacggcgtga ttagtgatta gtggttagtg attagtggat ggggccggca
     1561 gaattctgct ggccccatct gtttgtggtt gcctgctgga gcgggcgcat tgcaatgcgc
     1621 ccctacatga ggtcgtctgg tttgattgct accggggtgg cttcggctgg agtggagagg
     1681 gactggatga tgatgtccat ggcggccggg ccgggcatgg ggatgacgga gtcgatcgac
     1741 tgcggcacgc cggtgacgcc gagcgcggcg aggttgttga tgacgccggg gacggcggtg
     1801 cggaagccgt gattctccca gccgatccgc tggatctggg ggtcgcggag ggcctccatc
     1861 aaccgctgtc cgccgggggt gagggcgagg agcggctggg aggtccagac ggtcggcttc
     1921 ggatagagcg tgcgcacgtt ctggcggacg tagtcctgcg cgtcctgcga ggggtgagcc
     1981 aggttgtatt cgatcatgtt gctctcgtag gcggcgatga tcgggcaggc gccgatgccg
     2041 agcgacagga actgctcgaa caggagcgtc gtcgtgtccg gcaggagacc gagccgtccg
     2101 aagaaggctt gcagctccgg ggtgaccgcc tggaccgagg tctggtcgac cacgacacca
     2161 tcgttgagcg tgttggccag cagaccggcg aacgaattgc cggtgttgct ctgggtcggg
     2221 tcgctggtca ggacccggac cggcccgtag agctggggca gctcaatgtc cgaccagggc
     2281 gtgcctccgc tgatcatgtt gatgaggcgg ggcaaatcga cgatgtagta ggtatcgccg
     2341 gacttttcga cgatcccctg cttgaccagg gcgtcggcga tcggcgccca ggtgtacagg
     2401 acgagcggcg agttgaagat gatctcgttg gcgagcacgg cggtgccgag cctgttcttg
     2461 atgatttcgc cggaggtctg gctcgagggc cagcagtagt cgaggcccga ctggcactcg
     2521 ttgatcatct cgatcgagcc gacgcgcttg aaatcgaccg tcaggccgta tttctgcttg
     2581 agcagcgtgg tcacctccgg atcttgcaag aaggcgatct tgccgccgat gatgccggtg
     2641 accgtgatcg gatccggttc gggcgcgggc gcgcccgctc ccctatattc gggcgtgggc
     2701 cagaactcgg cggccgcgac gatgacggcg acgatcagga gaatggccag acctgcgtat
     2761 tgtcgtggtt tcatcgtcgg tctccgtcct gacgcaatgt ggcgatgccc tcaaggttga
     2821 attcgatcat ctcgctgatg gcgctgagac tggcgagggt ttcctggttg accttctccc
     2881 agagcaagtc gagcgctcgc tcgaggatgg cgagatcacg gagcagactc tcgtggacgt
     2941 cgtacccatc gagaccgcgc cggatcgctt tcgcgtaggg cgtcagcagc tcgatcatgg
     3001 tttccatcag gtcgaggagc atcggcgcgg cgtcgaattg cttgtcttcc tcaattgcgg
     3061 cgatcaactg gtcggtgcgg cagaggaccc gcgttatctg ggcagccggg cggccgtcgt
     3121 cgagtccgaa gctcagcatg gtgatgcttg gccgcagggt ttggcagagg gccagcgcat
     3181 cgcggccggt gcggggtcgg tggttcactg agtcggtgct tggggtgtgg ttggtcgagc
     3241 cggccatgag ccgcatccct ccataggcaa tcatcgagat gggcagcgca agccagagcg
     3301 atgccccgag cgcgagcacg aggagcagga ggagccctgc gatcaggccg cgaaccaggt
     3361 cctgctggag accgaggatg accgcgcgct gatatgtgct ggtcgagcga gccatggcgg
     3421 tctcctaggt gaatccccgg acggtccgga atgtggcggc gagattcgcg cggccatcga
     3481 agacgatgcc gtgggtgagg tcggcaatgg gctggagctg atccatggac gcgtcgccaa
     3541 aggtgattgc gaagaccggc accgcggcga ccgattctcg ggatatcacg gagtgaagat
     3601 cgccgtacgt tttgccattt gcgctgacgc cgtcggtcag gaggacgaca gaggcgaagt
     3661 agccgtcgat gccgtgctcc ttcatgatct ccagaccccg gatgaccggg ctgtagatgt
     3721 cggttccgcc accgcccttg actgcgcgga tctgctcatc gagttggcgc aattgggccg
     3781 ggtcattgcc ggtgacggtc caggtgtcga gaatcttgtc gtcgaacggg atgacgacgg
     3841 tgatgtcctg cggtccggtg tcgaggaggt attgggaggc ggattgtcgg tcgagcaaca
     3901 gatgcatcgc cgcgatgagt tggtccgccc cgccgttctc attcatgctg cctgagaagt
     3961 cgagcgcgaa gacggtgaac gacggcttgc ggagcgccga ttggtagagg ctgagcgctt
     4021 cctggacgac ggcggccccc gggacccgga tcggggtcag gatgcgctgg gtgtcgatac
     4081 cccagtccgg gttgaagacg gccgcgtcca cattgaccgg gtcggggttg acgccgattc
     4141 cggtccggcg gccgaggccg aggagctggt gctggatgtc gtctgactgg aggtgcgcct
     4201 gcagcttctg gaagagggcg tccttcttct tgtcgccctt ggcgatctcg ccgagcggga
     4261 agtcggcgat cgaggtgccg tcgcgcagat agagggcgta aagcggttct cgtccgccag
     4321 cggtgagggc ctggttggtc tcgatgatcg tcgactcgta gttgatcatc ccgtcgtagc
     4381 tgtcgtattc ggagaggaag agatccttga gcgagccgga gttgccggca ctgcggctga
     4441 cggtgccgag gatgcgcttg accttctcct gcacggcagg gttccgcagg tcggccgagg
     4501 tgaggacgtc gggctgaccg gcgaaggcgt agaggaagcc gaggtaggcg gccgcgccgg
     4561 agtcggactg ggcggcgtta gccatcatga aggtgaagtc gtcggcttcg gccgcggcca
     4621 ggacatcggc cacggtcaca tcggcgccgg cccagccgag ccgctcggcg accgattgct
     4681 tgacgccgag gacgaccggc gagcgcatga tgctgctcga gaactggacg gcgttggtcg
     4741 agtcgccgag caggatccag atgctgctgg ccggccagac cgcgtcgtag cgggagttcg
     4801 tgccgttggc cagctcgcgc atgatgtcga ccgagccggc gtagctgacc tcgatcttcg
     4861 ccttctggtc cctggcgaag gactggatga tcggctcgag cggctggttc tccgcgccgg
     4921 acaggacgcg gaaggtcgca tcgtaggctg ggcccgggcg atccacgcag gccgccagca
     4981 tgatcatgac gagcaggaac ccgaggatga aagacattcc gcgcaacggg tgccgaacat
     5041 cttcgtcaac gtcgggcggg acccaacaaa tcggccaggt catgtcgccg cctcccgaaa
     5101 tgcgccgggt tcgcggacag agacggccgg cgattccgag cgcgtcgtcg agaaggagtg
     5161 gttcgccatg gcgacgcctt gtgcccagga tgcgtcttgg gccgaaacgc ttttgcctgt
     5221 tgtgaatgtg tgatcagtat cagcggcgcg ccccgaatcc acaaggtgtg cgggcatgag
     5281 tgcgagagcg accatcggag ccagggcaag gcgagtccat cggttccagt gacggatcgg
     5341 aagcgtgcaa tcagcggcgt tgaggatgcg gatagcgagg gatcggaccc aggccggaat
     5401 tctcatttcg tggccttctc ccggttggcc gggatcgcgg ccggggtggt cgcgatcccg
     5461 atgctggcgg ttgcttcggt cgaggcgcaa ccggcccaaa gtccggtgcc ggccagatcg
     5521 gcggtcttgg cggtggcatc aaaccaggcg gtgaagcggg ccccggccgg caggattgcg
     5581 gcgtaggcgc tgccctcggc gatcaggcgc tggttgagca gggtgcgggt gccgtcgtcg
     5641 ttgaccagcc agacgtagcg gctggggacc gagccgtctg taatggctgg atccgactca
     5701 atcaggactg ttgcgccggc gaggtcatcg agcgtcgatt cggcagcgtt ggcgaagcag
     5761 gagccgtcgg ttggcgggtt gacgccgagc aggcggatcc gggatgaccg ctccttgccg
     5821 tcgagcatga cccggagcgt gttgccgtcc gtcgcggaga cgatctcgcc ttcggtcagg
     5881 ttcggcggac cggacgcggc ggggagagcg tcggctcgga ggtcggacgc gatgacgtcg
     5941 gccagcggca ggctctccga accgacggtg agcagcggcg attcggcgcc ggccgggatc
     6001 acgaaggcga cggcgacgcg agcggtctgg ccggctgcga tctccagcgt caagtcctcg
     6061 gcgaagggcg tggtgccgag cttgtcagca atggccggca tcgagctgcg ggcgatcctg
     6121 gccggcttct gaagcggatc ggtcccgatg gtgaagtcgt gggcggagag gacggcgtcc
     6181 tggtcgctcc agttggtgac gtcgaggact gcgaccagcc acgccttgtc cgtcttcttc
     6241 ctgaggccga ggtccggaaa ggtcgggcgg acggcggtgg tggcgacggc gactcgccag
     6301 gggccggaaa cgaagaccgg ggccggctcg ccatcgacca gcggcggatc ggaccgcgga
     6361 ctgtcgatgc cggggcgaac cgtgctgagc agcttcgcga attcgggctg ctgcgtatcg
     6421 aaggcgctcg ccggggtctc ggcgatgaag gcaaccacgg actcgcccgg cacgatgatc
     6481 cgagcctcga tgtaggtcgc caggagtatc tctctaccgt cgtcgagggt gagggtgtaa
     6541 gtgaaggccg cgaaggcgtg ctggtcgtcg ccaccccgga tgagttcgcc gctgtcggca
     6601 tgcatgggcg ccatgtcgct gactcccgat gacaggcgga ccgcgccgat gaacccggcg
     6661 agcgcggcct gcggggtggt cgaggaatca gggccgccga ggagcgtgca gtaggtcagg
     6721 ccgttggtga cgatgacccg gtcgaagatc tcgttcgagg tctcgtcggc gacgaaccag
     6781 gtgtcgtccc acgacatcga aaagccgaac tggggactgg tgtaggagtt gcccgagacc
     6841 tgggctgcgg ccgggtggac gaactggccg gggacgatca gcagcagcgg gatcaggatc
     6901 gcggccagca attggagcgt ccgggttctg cccgagaacg aggtgagcat gatgcgcctc
     6961 ccctgtgtcg tgagttcccg ggtggtggtc cgggagacgg cgctgaccga cagcgaccgt
     7021 ggccacagga tgagcgggga gggagcccgg gaacagacag ggatttacgt attttggggc
     7081 tggcgttgtc ctacgtacat cacgcgacag gcgtcagttc tggctcatcc gggggcgcga
     7141 gaccccagcg cacggcgaag gcgaccgcct cggcgcggtt gcggacccgg atcttgtcgt
     7201 agatctcggt caggtgggtg gtgaccgtgc gcttggagat gaagagcatg tccgcgatct
     7261 cctgatcggt cctgccggtg gtcaggatgc ggagaattgc gagctggcgc ggcgtcaggg
     7321 aggcgaccag cgcggcgacg tgtggtggat gggactgccc gacgctctcc ggaagctcgg
     7381 gcgggagtgt ggcgacatcg gccggaaccg ggagctgctc gatcgtctcc tcgatcacgt
     7441 cgctgagctt gccgagatcg acgacggtct cctggatcag ctcctgccag atctcgccaa
     7501 agccgtgatc gagcatgatc agattggcac ggacgcgttc cttcatctcg gcgggatcat
     7561 gcccgcgccg gagcaacttg gcgtagttgg tgaggaaggt atcggtgacg gtcagcggct
     7621 cgatcagctt gaggcagatt tcggggtgat tgtcctcggc aatcacctcc atgatctggt
     7681 cgatgtggat cacgatctgc tgggtcagct cgcgggtccg gatgtcgctc atcgttccac
     7741 agagggtggc gatgcgggct tgcaggcggc ggcactcgtc gtacgcgccg cggacctcgc
     7801 gggctgtc
//
LOCUS       DATAOZ010000661         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5779942, whole genome shotgun sequence.
ACCESSION   DATAOZ010000661 DATAOZ010000000
VERSION     DATAOZ010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5779942"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VHR64_16595"
     CDS             <1..453
                     /locus_tag="VHR64_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX3304507.1"
                     /translation="NVLAGAIAVGVTVLPMIASLSEDALRAVPRSLREAAYGLGATKL
                     EVSTRVVTPAALSGIVSAVILAVSRAIGETMIVALATGSKAQLTADPREPMQAMTGYI
                     VQVLSGDVQRGSTVYYSLFAVGLLLFCMTLAMNILSQWFVARFREEYQ"
     gene            450..1358
                     /gene="pstA"
                     /locus_tag="VHR64_16600"
     CDS             450..1358
                     /gene="pstA"
                     /locus_tag="VHR64_16600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX3304508.1"
                     /translation="MSAEATRMTGAPILTTSSQLRRRQIRGQVMRVLFLAATLVGIMA
                     LVVLLWTIFNRGLPWLSWHLITDMPSRKPELAGLNSALWGTIWVISLTALIAFPIGVG
                     AAIFLEEYAPRNRWTRALQVNISNLAGVPSVVYGLLGLGIFVQFMNLGRVVLAGALTM
                     ALLSLPVIIISSQEAIRAVPLSLRQAAYGVGATRWQVTRHHVLPAAFPGILTGTILAV
                     SRAIGETAPVLVVGASAYIATRPSGFFDGYTVLPMQIYGWTQRSQDQFKNGLAPAGII
                     ILLAVLLIMNAAAIILRQKLSKRSQL"
     gene            1355..2200
                     /gene="pstB"
                     /locus_tag="VHR64_16605"
     CDS             1355..2200
                     /gene="pstB"
                     /locus_tag="VHR64_16605"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX3304509.1"
                     /translation="MSAVLTRPVVEARLAPTSGPARANEAPEPAIIDIEDLNVFYGDF
                     HAVADVSFSVPRNCVTALIGPSGCGKSTVLRSINRMNDLIESARVEGEIRYHGVDLND
                     RRVDPVEVRRRIGMVFQKANPFPKSIYDNVAWGVKINGMKVNLDEHVEKCLRQAALWD
                     EVKDKLKESGLALSGGQQQRLCIARALAINPDIILMDEPCSALDPISTLKIEDLMRQL
                     VADYSIVIVTHNMQQAARVSDQTVFFSLNEQRQGILVEASPTTELFTNPRDKRTEDYI
                     TGRFG"
     gene            2221..2886
                     /gene="phoU"
                     /locus_tag="VHR64_16610"
     CDS             2221..2886
                     /gene="phoU"
                     /locus_tag="VHR64_16610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX3304510.1"
                     /translation="MTRQNYEHQIQQLREAVIGMSAMVDKAISRSTDALRYQDLELAQ
                     LVIDEDIIINRERWRTEELALLIIATQAPMAGDLRVISSVIHIATELERMADHAAGNA
                     KIALQTAYEPLLKPLVDIPCMADVSREMLTDSITALIQRDTELARKVAARDDEVDALY
                     NQIYRELLTFMMEDHSTISRATHLLWAAHNLERIADRVTNICERVVFAVTGSIEEIGV
                     SIY"
     gene            complement(2883..3335)
                     /locus_tag="VHR64_16615"
     CDS             complement(2883..3335)
                     /locus_tag="VHR64_16615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX3304511.1"
                     /translation="MTDYHRPHVLIVSDDLDLSQFLQSGLTIAGFWTSIVASALQTLE
                     LFRLRTFDLMLIDALLEGLGAGELVQRLRTPVEPGGPPRTDIPILLIAGSEAETSAER
                     ANEMGASGVIYAPIEIDDLALDLFDRVKQWRELHPGRPWADELAQRTS"
     gene            3508..4125
                     /locus_tag="VHR64_16620"
     CDS             3508..4125
                     /locus_tag="VHR64_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX3304512.1"
                     /translation="MTVVLENVHDPHNIGAVLRSCDAVGIMAVHTIYSIEERPARAYA
                     RTTSGSASKWIDVHHHDSVEDCYAALRAQGFRILVTALTETSVDLYDTSFVEPTAIVF
                     GNEMRGATAEAIEQADGALLIPMMGMVESLNISVACAVTLFEALRQRRAAGLYDQPSL
                     SDAQLEQLQVDWLAREVSVQETALVCLFLSRVAVTAHAFRKPVSN"
     gene            complement(4039..4278)
                     /locus_tag="VHR64_16625"
     CDS             complement(4039..4278)
                     /locus_tag="VHR64_16625"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX3304513.1"
                     /translation="MIFTIGRHVKGLTTAAGVWVAAAVGLLIGSGFYIIGFGGVVMTV
                     LALRVFGSIAHRFPEGVRRNRDAGEEEAHERGLLD"
BASE COUNT          838 a         1404 c         1290 g          807 t
ORIGIN      
        1 aacgtcctgg ccggtgcgat tgcggtcgga gtgacggtct tgccgatgat cgcttccctt
       61 tccgaggatg cgctgcgagc cgtgccccgt tcgctacgcg aggcggccta tggactcggc
      121 gcaacgaagc tggaagtttc gacccgggtc gtgacccctg cagcgctctc cgggatcgtc
      181 tccgccgtca tcctcgctgt ttcgcgggcg attggcgaga cgatgatcgt cgcgctcgcc
      241 acaggctcaa aggcgcaatt gaccgccgac ccccgcgagc caatgcaggc tatgaccggc
      301 tacatcgtcc aggtcctgtc cggcgatgtc cagcgcggct cgacggtcta ctactccctc
      361 ttcgcggtcg gcctgttgct cttctgcatg acgctggcca tgaatatcct gagccagtgg
      421 tttgtcgccc gcttccggga ggaatatcaa tgagcgccga agcgacccga atgacgggtg
      481 cgccgattct caccacatcg tcccagctcc gtcgtcgcca aatccgcggg caggtcatgc
      541 gggtgctttt cttggccgct accctggttg ggatcatggc cctggtcgtg ctgctttgga
      601 cgatcttcaa tcgtggtctg ccgtggttga gttggcatct catcactgac atgccgtcgc
      661 gcaagccgga gctggcggga ctgaactcgg cgctttgggg cacgatctgg gtgatctcgt
      721 tgacggcctt gatcgcgttc ccgatcggcg tcggcgccgc tatcttcctc gaggagtacg
      781 cgccccggaa tcgctggacc agggcactcc aggtcaatat ctcgaacctg gccggtgtgc
      841 cttctgtcgt ctacggattg cttgggctcg gcatcttcgt ccagttcatg aatctcggcc
      901 gggtcgtcct ggccggcgcg ctgacaatgg cgctgctcag cctgccggtc atcatcatct
      961 ccagccagga ggcgatccgc gcggttccgc tctcactccg gcaagccgcc tacggagtcg
     1021 gagcgacgcg ctggcaggtg acacgtcacc acgtccttcc ggccgcgttt ccgggaatcc
     1081 tgaccggaac gatcctagcc gtctcccggg caatcggcga gaccgcgccg gtactcgtcg
     1141 tcggcgcgtc tgcttacatc gcgacccgcc catccggctt cttcgacggc tacaccgtgc
     1201 tgccgatgca gatttacgga tggacgcagc gctcccagga tcagttcaag aacggactcg
     1261 ccccagccgg gatcatcatc ctgctcgcag tcctgctcat catgaatgcc gccgcaatca
     1321 tcctccgcca gaaactcagc aaaaggagtc agttatgagt gcagtcctaa ctcgcccagt
     1381 ggtggaagcc aggctcgcgc cgacaagtgg tccagcccgg gcaaacgagg cgccggaacc
     1441 ggcaattatt gatatcgagg atctcaacgt cttctacggc gacttccatg ccgtcgcaga
     1501 tgtctcgttc tcggttccgc gcaattgcgt cacggccctg atcggcccgt ccggctgcgg
     1561 caagagcacc gttctgcgta gcatcaaccg gatgaacgac ctcatcgaga gcgcgcgagt
     1621 cgagggcgag atccgctacc acggcgtgga cctcaacgat cgccgggtcg atccggtcga
     1681 ggttcgccgg cggatcggga tggtcttcca gaaggccaac ccgttcccca agagcatcta
     1741 cgacaacgtg gcgtggggtg tgaagatcaa tgggatgaag gtgaatctcg acgagcatgt
     1801 tgagaaatgc ctgcgtcagg ctgcgctctg ggacgaagtc aaggacaagc tcaaggagag
     1861 cggtctcgcc ctttccggcg gccagcagca gcggctctgc atcgcccgcg cgctcgccat
     1921 caaccccgac atcatcctga tggacgagcc atgcagcgcc ctggacccga tctcgacgct
     1981 caagatcgag gatctcatgc ggcagttggt tgcggactat tcaatcgtga tcgtgacgca
     2041 taacatgcaa caagcggccc gcgtctcgga ccagacggtg ttcttctcgc tgaacgagca
     2101 acggcagggc atactggtcg aggcgagccc gaccactgag ctcttcacga acccgcgcga
     2161 caagcgcacc gaggattaca tcaccggtcg attcggctag tccggcccaa cggagcattc
     2221 atgacccggc agaactacga gcaccagatt cagcagctcc gtgaagcagt catcggcatg
     2281 tcggcaatgg tcgacaaggc gatcagccgg tcgaccgatg cgctccggta ccaggatctg
     2341 gagcttgccc agcttgtcat cgacgaggac atcatcatca atcgcgagcg gtggcgcacc
     2401 gaggagttgg cgctgttgat cattgccacg caggccccga tggccggcga cttgcgggtg
     2461 atctcgtcgg tcatccacat cgcgaccgaa ctcgagcgga tggcggatca cgcggccggg
     2521 aacgccaaga tcgcgctgca aaccgcctat gagccgcttc tcaaaccgct tgtcgatatc
     2581 ccgtgcatgg ctgacgtctc gcgggagatg ctgaccgaca gcatcactgc cttgatccag
     2641 cgcgacaccg agctcgctcg caaggtcgct gcccgggacg acgaagtcga cgcgctctac
     2701 aaccagattt accgcgagct gttgaccttc atgatggagg accactcgac gatcagccgg
     2761 gcgacgcacc tgctctgggc ggcgcataac ctggagcgaa tcgccgatcg cgtgaccaac
     2821 atctgtgagc gcgtggtctt cgcggttacg ggctcgatcg aggagatcgg cgtctcgatt
     2881 tattaggacg tccgctgggc caactcatcc gcccagggcc gacccgggtg caactcccgc
     2941 cactgcttga ccctgtcgaa aagatccagc gccaaatcgt cgatctcgat aggggcgtag
     3001 atcacgccgc tcgcacccat ctcgttggcc cgctccgcgc tggtctcagc ttcggacccg
     3061 gcgatcaata ggatcggtat gtcggtgcgc ggcggacccc cgggttcgac cggtgtacgc
     3121 aagcgttgca ccaactctcc ggcgccgaga ccttcgagaa gcgcatcgat cagcatcagg
     3181 tcgaacgtgc gcagccgaaa caactcgagc gtttgcagcg cgctggcgac gatcgaggtc
     3241 cagaacccgg caatggtcag gccagattgc agaaactggc tgagatccag gtcgtctgac
     3301 acaatcagaa cgtggggtcg gtggtaatca gtcatctgtt gcaggcccgc acctgaggaa
     3361 tcgtttcatg agggaatggt gccacgatcg atgcgagcgg caaaccggca agctcttccg
     3421 ccggatccga ccaagaccag caagttcccc cgtaccagcc gcagagccga gcgcattgcc
     3481 catgttctga gtcgacgaca gccggatttg accgtcgtcc ttgagaacgt tcacgacccc
     3541 cacaacatcg gcgcggtgct tcgctcatgc gacgcagtgg gaatcatggc agtgcacacg
     3601 atctactcaa tcgaggagcg tccagcccgg gcgtacgccc gcacgaccag cgggagcgcc
     3661 agcaaatgga ttgacgtcca ccaccatgac tcagtcgaag actgctacgc ggcactgcgt
     3721 gcgcagggat tccgcatcct cgtcactgcg ctgaccgaaa ccagcgttga cctctacgac
     3781 acgtcatttg tcgagccgac cgctattgtc ttcggaaacg agatgcgggg agccaccgcc
     3841 gaggccatcg agcaggcgga cggggcactt ctaattccga tgatgggcat ggtcgagagc
     3901 ctcaacattt cggtcgcctg cgcggtgacg ctcttcgagg cactgcgcca acggcgagcg
     3961 gccggtcttt acgatcagcc ctcactctca gacgcacagt tggagcagtt gcaggtcgat
     4021 tggttggccc gagaggtgtc agtccaggag accgcgctcg tgtgcctctt cctctcccgc
     4081 gtcgcggtta cggcgcacgc cttccggaaa ccggtgagca attgacccaa acacgcgcaa
     4141 ggcaagtaca gtcatcacga ctccaccaaa tccgatgatg taaaaaccgc tgccaatcag
     4201 caatccgacc gcggccgcaa cccaaactcc ggccgccgtg gtcaaccctt taacatggcg
     4261 gccgatcgtg aagatcacgc ctcctgccag aaacccgatg ccctggacaa cggtcgacgc
     4321 aatccgcgac ggatcgaca
//
LOCUS       DATAOZ010000662         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2887850, whole genome shotgun sequence.
ACCESSION   DATAOZ010000662 DATAOZ010000000
VERSION     DATAOZ010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2887850"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2065)
                     /locus_tag="VHR64_16630"
     CDS             complement(<1..2065)
                     /locus_tag="VHR64_16630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX3304514.1"
                     /translation="MSVDHLADQPADPSSDRPSSEPVLYNEILILLGILMVAGWLRFT
                     GIEWGKNLYLHPDERFQTMVVVAAKWPSSLSEYFDSQASPLNPYNNGFGSYIYGTFPL
                     FASKLIGTITGNVVYGDAHLPGRAFSAVCDLVTVLLVYLTGRRLFGRRTALLAAALCT
                     VTVLQIQSAHYFTSDSAVVVMCLAAFYLALRAESSGAWGWNVACGIAVGLAIASKINA
                     LPIALVMALPVFERWRRHGVRSIWRAGSDKPLAPVAGLVLAVLAALWTFRIAQPYAFA
                     GPSPFSFRLDPRWVKDVRYWRDVQNGVGDSPPSMQWADRTPILFSSRNLVLWGMGPLL
                     GLTAIAALVAGGFKLVAARRMPEGWQVILVGWPAFHLLYYGSGFVQTMRYLLPAYPFL
                     TLLAAAFLIRVLDLGTARGWGSVRIGRRISLPWAALPAVIVLAGTFLYAVGFVGIYTR
                     TTTREAASRWIYANVPPGSTIASEHWDDGIPVSLPGHDTNLYPGFQLELFFPDSAEKL
                     DTLVGQLDDADYLVVTSNRLYATIPRIPERYPMTSEYYRMLFSGDLGFDLIHTETSYP
                     TYLGIELNDDRAEEAFTVYDHPKVFIFRKNDSYDASAIHSRLDAAFGDGAYAIAPINA
                     GKHLLMMDDETRVQQQTSGTWRTMFNANSLANRYPTIIWYLVLQIMALASVPFCWRLF
                     DSLPDR"
     gene            complement(2062..>2717)
                     /locus_tag="VHR64_16635"
     CDS             complement(2062..>2717)
                     /locus_tag="VHR64_16635"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX3304515.1"
                     /translation="QDRLDQCRRQLGDRSRVSYVQGDAQALPFPAESFSRAVCSEVLE
                     HLDDDRAAMAELFRVLRPGGVLALTVPSADYPAAWDPLNYLLEGTVKRHLGGERPWSG
                     IWYGHKRLYTIVQLRALARDAGFVIEEERPLTHACPPFAHLILYGTLKPLLMRGLLPG
                     SLTSAGDRLSSGPKPPRELTALAMRLLERIDRGNDDPDIADRARTFVALSIKARKPG"
BASE COUNT          546 a          820 c          920 g          431 t
ORIGIN      
        1 cgcgatcggg gagcgaatcg aacaggcgcc agcagaacgg gaccgacgca agggccatga
       61 tttgcaggac cagataccag atgatcgtgg ggtagcgatt ggcaaggctg ttcgcgttga
      121 acatcgtccg ccaggtcccg cttgtctgct gctgaacgcg cgtctcgtca tccatcatca
      181 gcaagtgctt gccggcgttg atcggcgcga tcgcatatgc gccgtctccg aatgccgcat
      241 ccagcctgct gtggatagcc gaagcgtcgt acgagtcgtt cttgcggaag atgaagacct
      301 tgggatggtc gtagaccgtg aaggcttctt cggcgcggtc gtcgttgagc tcgatgccga
      361 ggtaggtcgg gtacgacgtc tccgtgtgga tgagatcgaa tccgagatcg cccgagaaca
      421 gcatccggta atactcggac gtcatcggat atcgctcggg gatgcgtggg atcgtcgcgt
      481 agagccggtt gctggtgacg acgaggtagt cggcatcgtc gagctgtccg acgagcgtat
      541 ccagtttctc ggctgagtcc gggaaaaaga gctcgagttg gaagcccgga tataggttcg
      601 tatcgtgacc cggaagcgag acgggaatcc catcgtccca gtgctcggat gcgatcgtcg
      661 agcctggcgg aacgttggcg taaatccagc gtgaggccgc ttcgcgagta gtggtccgcg
      721 tgtaaatccc gacaaagccg acggcgtaca ggaatgtccc ggccaacacg atcacggccg
      781 gaagcgcggc ccacggcaga gagatgcgtc gtccaattcg caccgatccc catcctcggg
      841 cggtgcccag gtcaagcacc cggatgagaa acgccgccgc gagcagggtg aggaacgggt
      901 atgccggcag cagataccgc atcgtctgaa cgaagccgct cccgtagtag aggagatgga
      961 aggccggcca acctacgaga attacctgcc aaccctcagg cattcgcctc gccgctacga
     1021 gcttgaatcc gccagcgacc aatgcggcga tcgcggtcag cccgagcaac ggtcccatgc
     1081 cccagagcac gagattccgc gacgaaaaga gaatcggcgt gcgatcagcc cactgcatcg
     1141 acggcggaga atcgccaacg ccattctgga catcgcgcca gtaccggaca tccttcaccc
     1201 agcggggatc gagccggaag ctgaacgggc ttgggccggc gaaggcgtag ggctgcgcga
     1261 tccgaaatgt ccagagcgcc gcgaggactg ccaggacgag gccggccacc ggcgcgagcg
     1321 gcttgtccga tccggcgcgc cagatcgagc gaacgccatg tctccgccac cgctcgaaga
     1381 ccggcaacgc catgaccagc gcgatcggca gcgcgttgat cttcgaggca atcgccagtc
     1441 cgaccgcgat cccgcaggcg acgttccagc cccacgcccc gctcgattcc gcccggagcg
     1501 cgaggtagaa cgcggccagg cacatgacca ccacggccga atccgacgtg aagtagtgag
     1561 ccgactgaat ttgcagaact gtgaccgtgc acagggcggc cgcaagcaag gcggtacgtc
     1621 ggccgaacag ccgccgtccc gtcaggtaga cgagcaggac cgtaaccagg tcgcaaacgg
     1681 ccgaaaaggc gcgacccggg agatgggcgt cgccgtagac cacgttcccg gtgatggtgc
     1741 cgatcagctt ggaggcgaag agcgggaatg tgccgtagat gtaggagccg aagccgttgt
     1801 tgtacgggtt gagcggagag gcctggctgt cgaagtactc gctgagactc gacggccatt
     1861 tcgccgcgac gacgaccatc gtctggaagc gctcatccgg gtgcaggtag aggttcttgc
     1921 cccactcgat gccggtgaag cggagccatc cggccaccat caggatgccg agcaggatca
     1981 ggatttcgtt gtagagaacc ggctcggacg acgggcgatc ggatgacggg tcggcgggtt
     2041 gatccgccag gtgatcgacg ctcatcccgg tttgcgtgcc ttgatcgaga gcgcgacgaa
     2101 ggtgcgggcg cgatcggcaa tgtcaggatc gtcgttgccg cgatcgatgc gctccaaaag
     2161 tcgcatcgcc agtgcggtca actcgcgcgg tggctttggc ccagagctca ggcgatcccc
     2221 cgcgctggtc aggctgcccg gtagaagacc gcgcatcagg agcggcttga gcgtaccgta
     2281 gaggatcagg tgcgcgaagg gcgggcaggc gtgcgtgagc gggcgctcct cctcgatcac
     2341 gaagccggca tccctggcaa gcgcgcggag ctggacaatc gtgtagagcc gcttgtgacc
     2401 gtaccagatg ccggaccacg gtcgctcgcc gccgagatgt cgcttgaccg tcccctcgag
     2461 caggtaattg agcgggtccc aggcagcggg gtagtcggcc gatggcacgg tcagggccag
     2521 cactccgcca ggccggagga cgcggaagag ctcggccatc gccgcgcggt cgtcatcaag
     2581 atgctcgagg acttccgaac agacggcgcg gctgaacgac tctgcgggaa acggcagcgc
     2641 ctgcgcatcc ccttggacat aggagacccg cgaccggtcg ccgagttggc ggcgacattg
     2701 atcgagcctg tcctgat
//
LOCUS       DATAOZ010000663         8387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_8677192, whole genome shotgun sequence.
ACCESSION   DATAOZ010000663 DATAOZ010000000
VERSION     DATAOZ010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8677192"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VHR64_16640"
     CDS             <1..1105
                     /locus_tag="VHR64_16640"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3304516.1"
                     /translation="EINEPIKEQLDRMIHSSTLFLVRAQIELAERIRSMTPPKLNKVF
                     FTNSGTEANEAAFLITTLARSSNELIALRHSYHGRSFATINASGQRTWRSSTLNPLSV
                     HYVGNPYCYRCPWEKTYPSCDLVCARDVESVIQTVTSGHPAAFIAEPIQGVGGFVTPP
                     PDYFVRVSEILQKYGIPFIADEVQAGWGRTGEADFGFQAYGVEPDVVVFAKGLANGVP
                     IGGLIATDELASSIKSLSLSTFGGNPIATTAALANLNYIATNRLRENAREVGAYLNQR
                     LRELQTKHAVIGDVRGMGLMQAIEFVVDPVTKEPATELTARLHDEAKRRGLLVGKAGL
                     NANVFRLSPPLIISKADVDEAIGILDKSLTAVA"
     gene            complement(1108..2136)
                     /locus_tag="VHR64_16645"
     CDS             complement(1108..2136)
                     /locus_tag="VHR64_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX3304517.1"
                     /translation="MIDLSSDTATKPSEGMRAAMARAEVGDEQRREDPTVNALQDLVA
                     DLTGKEAGLFLPSGTMGNVIALAVHCRRGDSVIVDKTCHIATAEGGGSAFIAQVMLKP
                     VVGQRGVFCPEQIRAELTVEGTHTSPTRLISVENTTNRGGGKVWPLDALAGVRKVADE
                     AGAIVHMDGARLMNAVIAGGYTARDAASYVDSVWVDLSKGLGAPVGAVLCGTSEFIEE
                     ARLLKHRLGGAMRQAGVIAAAGLYAFEHNVERLAEDHENAKLLEAGIAGIDGLEPVFG
                     PVETNIVFFDVSKSGKGAAEICAAMEREGVRMSQYPDPTKIRAVTHLDVSRADCERAV
                     EVLRSVVA"
     gene            2262..3632
                     /locus_tag="VHR64_16650"
     CDS             2262..3632
                     /locus_tag="VHR64_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX3304518.1"
                     /translation="MATEITQQTLAVNPATGAALVREYEARTPRSKELAARAQKVMPG
                     GETRTITFHPPYPLTGDHGSGCQFTDVDGNVYYDLINNYTSLLHGHAHPVINAAVQKQ
                     LARGTTFATALEAQTELGEIMAERVASVDMIRYTNSGTEATMNAVRAARAFTGRELIV
                     KMEGGYHGSFDDFEISVHPDLTLAGPPDEPTPVIDTRGVVHSTLREVLISPFNNVEAL
                     THLFTQYGKDIAAVIIEPVMGAAGCIPAERDFLHAAQDLARQHGAIFIADEVMTFRLE
                     YGGVQEHYGISPDMTTFAKIIGGGFPVGAFGGRTEVMSIFDARNPNAVYQSGTFNGNA
                     ITTVAGIAAMNLFPRDEVARLNALGDQLRDGLVRELGSHGLPGVATGYGSMIGLHLVD
                     GDVRNYRDGAVANATFKRALHLALLLEGIFAAPRLMFCMSTPMTPETVDDMIARFGRA
                     LDRTLD"
     gene            3678..4397
                     /locus_tag="VHR64_16655"
     CDS             3678..4397
                     /locus_tag="VHR64_16655"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX3304519.1"
                     /translation="MSDAGLTPNQINLLAVSDQVDPRIHSASLRQRMPDVDIVFGCGD
                     VPARYLEFLADALDRPVYFVLGNHHEELTRKGHFGARYEPMGCTDLGGKVIRDRSTGL
                     ILAGFPGSPKYSRETDQQYSDHEIRWMMVKMMPRLLWNRLRRGRYLDVLISHAPPRDI
                     NDRDDLPHRGFPPLRTFLRWFRPAVHLHGHIHIYDRNEVVETPFHDTMVINVYPYTRI
                     DLVTQPCARAERIAPPGEPCG"
     gene            4390..5301
                     /locus_tag="VHR64_16660"
     CDS             4390..5301
                     /locus_tag="VHR64_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304520.1"
                     /translation="MVEEQVRTDFDRARHKAFRNELLSYFSRRPNALLSFHEVRKRYK
                     PEQESYRGMQEVPVRQIIGSVDRFRDFDRAFLPRHARSAGRWQNVDRAYYQDVRLPPV
                     QLYKVGDVYFVKDGNHRVSVARERGVEFIDAEVIESHVRVPIDASMTPFQLLMQVEYA
                     EFLRLTNLDRLRPEHDIRPSALGRYDELWDHILLRQLEMSDAQGRDVPIGEAVASWYD
                     DIYQPIVQIARERGVLQRFPDRSETDIYLWVMAHRDVLMEREDQEVAPVDAVQDLANE
                     IAVADSGFRLGALRRRLARWIAGPHDG"
     gene            complement(5315..5851)
                     /locus_tag="VHR64_16665"
     CDS             complement(5315..5851)
                     /locus_tag="VHR64_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304521.1"
                     /translation="MIRARLNLGLAALLLPAVLLGACGGGGKKEPTATAVPGTSVAVD
                     TAVTEGTLASVGSSATDGVCQVTIPDTWVDDGTGRGVTAQSDKWLVFGNMIGSDEAWN
                     SARDLLKSQMSGRAGAEIKETDDSITVTLANGRGYVDRVRFKDRYCEFSVMSTRDQPD
                     TVTTTWHSVADTLAPYEE"
     gene            complement(5848..6900)
                     /locus_tag="VHR64_16670"
     CDS             complement(5848..6900)
                     /locus_tag="VHR64_16670"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX3304522.1"
                     /translation="MTGSIALGRPSMVSARMMARDILKTMPLRDRVAQMFFFEARGTY
                     MTSTYSSDLTRVMPGGILFVANNISTAPALTTYINGIHASNPVIPPLICVDQEGGPVT
                     RLAGDPAPGAVQMGALSNSDVRTLNKERAAFLAAYGFDVNFAPVADVAYDASSTMAAR
                     SFGNDPKTVAEKVSATVKGSRAGGVIGAAKHFPGHGRTQVDSHKLIPEIDVPWDEWLK
                     TDAIPFESAIKAQVDMVMLGHLRYDRIDYKPMSISKVAVRALRKDLGFNGVIVTDDLG
                     MGALDDWKPLDIISQAIDAGIDVMLYGSPPVGWDALIDHVMDMISNGLVDRQDINGRV
                     EKIVALKLRHFGDRIS"
     gene            7168..8202
                     /locus_tag="VHR64_16675"
     CDS             7168..8202
                     /locus_tag="VHR64_16675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3304523.1"
                     /translation="MASSRNVRVGLAGLGNFGRLHARILSQLGSVDLAAVCDPDSDAR
                     TWAAETLGATTTVGDFDDLLRVPELDALFIVTPEDLHYGHVLKALTLGIPIFMEKPLS
                     TNAADARRVVDAARAAGTFLQIGFVVRFDAQHAMLRNRIVAGELGSIVLFRAKRNCSR
                     SWFATYGDRAHTVYESIIHDIDLALWFTQCRCTKVYAVQRNISGLTYPDALAAMLQLE
                     NGSVVMLDTSWFVPDQAPQNVIAGDWTGTIDAEFELIGTEQTARYRLLDSGMSIATPD
                     QIIHPEVGLWPEVYGSIGGALRLEDEHFISCVRSGRPSTIASLDDALHGLEIAEAIVH
                     SAESGLEVTL"
BASE COUNT         1533 a         2850 c         2526 g         1478 t
ORIGIN      
        1 ggagatcaac gaaccgatca aggaacagct cgatcggatg attcactcct cgacgctgtt
       61 cctggtccgc gcccagatcg agctggccga gcgcattcgc tcgatgacgc cgccgaagct
      121 gaacaaggtc ttcttcacca actccggcac cgaagcgaac gaggcggcgt tcctgatcac
      181 gaccctggcc cgctccagca atgagctgat cgcccttcgc cactcctatc acggccgctc
      241 cttcgcgacg atcaacgcct ccggtcaacg aacctggcgc tcctcgaccc tgaacccgct
      301 cagcgtccat tacgtcggca atccctactg ctatcgctgc ccgtgggaga agacctaccc
      361 ctcctgcgac ctggtttgcg cgcgcgatgt cgaatcggtg atccagacgg tcaccagtgg
      421 ccatccggcg gcctttatcg ctgaaccaat tcagggcgtc ggcgggttcg tcactccgcc
      481 acccgattac tttgtccgcg tctcggagat cctgcagaaa tacgggatcc cgttcatcgc
      541 cgatgaagtg caggccggct ggggacggac cggcgaggcc gacttcggct ttcaggcgta
      601 cggcgttgag ccggacgtgg tcgtcttcgc caaggggctc gcaaatggcg tcccgatcgg
      661 tggcttgatc gcgaccgacg agctggcctc atcgatcaag tcgctcagcc tctcgacctt
      721 cggcggcaac ccgatcgcga cgacggccgc gctggccaac ctcaactaca tcgcgaccaa
      781 ccggctgcgg gaaaacgcgc gcgaggtcgg cgcatatctg aaccagcgcc tgcgcgagct
      841 gcagacgaag cacgcggtca tcggcgacgt gcggggcatg ggtctgatgc aggcaatcga
      901 gttcgtcgtc gacccggtca ccaaggaacc ggcgacggag ctgaccgcgc gcctgcacga
      961 tgaggcaaag cgacgcggtc ttctggtcgg caaggcgggc ctgaatgcca atgtcttccg
     1021 gctcagcccg ccgctcatca tctcgaaagc agatgtggac gaggcgatcg ggatcctcga
     1081 caagtccctg accgccgtcg cctgaactta cgcaacgact gagcgcaaca cctcgactgc
     1141 ccgttcgcaa tcggcgcggc tgacgtcgag gtgcgtgacg gcccggatct tggttgggtc
     1201 gggatattgc gacatccgca caccctctcg ctccatcgcc gcgcagatct cggccgcacc
     1261 cttgcccgac ttgctgacgt cgaagaagac gatgttcgtc tcgaccggac cgaagaccgg
     1321 ctccagccca tcgatcccgg caatgcccgc ctcaagtagc ttggcgttct cgtggtcctc
     1381 tgccagccgt tcgacgttgt gctcgaaggc gtaaaggccg gccgcggcga tcacaccggc
     1441 ctgccgcatc gccccaccca accggtgctt gagcagccgt gcctcttcga tgaactcgga
     1501 cgtgccgcag agcaccgccc caaccggcgc gccgagcccc ttggagaggt cgacccagac
     1561 ggaatcgacg tacgacgccg catcccgcgc ggtatacccg ccggcgatga ccgcgttcat
     1621 cagccgcgcg ccatccatgt ggacgatcgc gcccgcctca tcagcgacct tccgaacacc
     1681 ggccagtgca tcgagcggcc aaaccttgcc accaccccga ttggtcgtgt tctcgaccga
     1741 gatcaaccgg gtcggcgagg tgtgcgtgcc ctcgacggtc aactcggcgc ggatctgctc
     1801 cgggcagaag acgccccgct ggccgaccac cggcttgagc atcacctgcg cgatgaaggc
     1861 cgatccccca ccttccgctg tcgcgatgtg gcaggtcttg tcgacgatga ccgaatcacc
     1921 tcgccggcaa tgaaccgcca gcgcgatcac attgcccatc gtcccggacg gcagaaagag
     1981 gcccgcctcc ttgccggtca gatccgcgac gaggtcctgc agcgcgttga cggttgggtc
     2041 ctcgcgccgc tgctcatccc cgacttcggc gcgggccatg gcggcgcgca tgccctcaga
     2101 cggcttcgtt gcggtatcgc tcgaaagatc aatcactcgt actgtccaat ccattacatt
     2161 ccgatagatc tacgccgaat tctatgcgcc ccgatgcctt gctgggtgat aggatcacgc
     2221 cagacgcgaa ccaaacgatt tcgaccggag aatcaacgcc gatggccacc gagatcaccc
     2281 agcagacgct agcagtaaac ccggcgacgg gcgcggcgct ggtccgcgag tatgaggcgc
     2341 gcacgccacg ctcgaaggag ctggctgcgc gggcacagaa ggtcatgccg ggcggcgaga
     2401 cccgcaccat caccttccac ccgccgtacc cgttgaccgg cgatcacggc tccggctgcc
     2461 agttcaccga tgtcgacggc aatgtctact acgacctgat caacaactac acctcgctcc
     2521 tccatggtca tgcccacccg gtcatcaacg ccgcggtcca gaagcagctc gcccggggca
     2581 cgacgttcgc gaccgcgctg gaagctcaga ccgagctcgg cgagatcatg gccgagcggg
     2641 tcgcatcggt cgacatgatc cggtacacca actccgggac cgaagcgacc atgaacgcgg
     2701 tccgcgccgc ccgcgccttc accggtcgcg agttgatcgt caagatggag ggcggctacc
     2761 acggcagctt cgacgacttc gagatcagcg tccaccctga cctgacgctt gccggaccac
     2821 ccgatgagcc gacgccggtg atcgacacgc ggggcgtcgt tcatagcacg cttcgcgaag
     2881 tgctgatctc gccgttcaac aacgtcgagg cgctgaccca tctcttcacg cagtacggca
     2941 aggacatcgc cgccgtcatc atcgagccgg tgatgggcgc ggccggctgc atcccggccg
     3001 agcgcgactt cctgcacgcc gctcaagacc tcgcccggca gcatggcgca atcttcatcg
     3061 cggacgaggt gatgaccttc cgcctcgagt acggcggagt ccaggagcat tacggaatct
     3121 cgccggatat gacgacattc gccaagatca tcggcggcgg tttcccggtc ggcgcgttcg
     3181 gcggtcggac cgaggtgatg agcatcttcg acgcccgcaa cccgaacgcc gtctaccagt
     3241 ccggcacatt caacggcaac gcgatcacca cggtcgcggg aatcgcggcg atgaacctct
     3301 tcccgcgaga cgaggtcgcc cgcctgaacg cgctcggcga tcagttacgc gacgggctcg
     3361 tccgggagtt ggggagccac ggtctgcccg gcgttgccac gggttatggg tccatgatcg
     3421 gcctgcacct ggtcgacggc gacgtccgaa actatcgcga cggcgccgtc gcgaacgcca
     3481 cattcaagcg tgcgctccac ctggccctgc tactcgaagg catcttcgcg gcgccacgct
     3541 tgatgttctg catgtcgacg ccgatgacgc ccgagaccgt cgacgacatg atcgcccggt
     3601 tcggccgtgc cctcgatcgc acattggatt agaccagcgc tcctacagaa ctggacacgg
     3661 ggcggtatgg tttcggcatg tcggatgccg gcctcacgcc caaccagatc aacctgctcg
     3721 cggtttcgga ccaggtcgac ccgcgcatcc acagcgcatc actccgacag cgcatgccgg
     3781 acgtcgatat cgttttcggc tgcggtgacg tcccggcccg gtacctcgag ttccttgccg
     3841 acgcgctcga ccggccggtc tatttcgtgc ttggtaacca ccacgaggag ctgacgcgca
     3901 aaggacactt cggggcgcgg tatgagccga tgggctgcac cgatctcggc ggaaaggtca
     3961 tccgagatag gtcgaccggc ctgattctcg ccggcttccc cggatctccg aagtacagcc
     4021 gcgaaaccga tcagcagtac agcgaccatg aaatccggtg gatgatggtc aagatgatgc
     4081 cgcgcctcct ctggaaccga ctccgccggg gccgatacct cgatgtcctg atctcccatg
     4141 cgccgccccg cgacatcaac gaccgggacg atcttccgca ccgcggcttt cccccgcttc
     4201 ggaccttcct gcgctggttt cgaccggccg ttcatctgca cggccatatc catatttacg
     4261 atcgcaacga ggtggtcgag acaccatttc atgacacgat ggtgatcaac gtctatccct
     4321 atacgagaat tgacctcgtg acgcagccgt gcgcccgcgc cgagcgaatc gcgccgccag
     4381 gtgagccctg tggttgagga acaagtccgc accgattttg atcgcgcccg ccacaaggcg
     4441 tttcgcaatg agctgctctc ctatttcagc cggcggccca acgcccttct ctccttccat
     4501 gaggtccgca agcgatacaa gcccgagcag gaaagctacc ggggcatgca ggaggtgccg
     4561 gtccgccaga tcatcggcag cgtcgatcgc ttccgtgatt ttgaccgcgc ctttctgccc
     4621 cgccatgccc gctcggccgg gcgctggcaa aacgtcgatc gtgcctacta ccaggatgtg
     4681 cgcctgccgc cggtgcagct ttacaaggtg ggcgatgtct acttcgtcaa ggacggtaat
     4741 caccgcgttt cggtcgcgcg ggaacgtggc gtggagttca tcgacgccga ggtgatcgag
     4801 agccacgtcc gggtgccgat tgacgcctcc atgaccccgt ttcaactcct gatgcaggtc
     4861 gagtacgccg agtttctgcg gctgaccaat ctcgaccgcc tgcggccaga gcacgacatc
     4921 cggccgagcg cgctcggtcg ctacgacgag ctctgggatc acatcctgct ccgccagctc
     4981 gagatgagcg atgcgcaggg acgtgacgtg ccgatcggcg aagcagtcgc cagctggtat
     5041 gacgatatct accagccgat cgtccagatc gcccgcgaac gaggcgtgct gcagcgcttc
     5101 cctgaccgat ccgagacaga tatctacctc tgggtcatgg cccaccgtga tgttctgatg
     5161 gagcgggagg atcaggaagt cgcgccggtc gacgccgtcc aggacctggc caacgagatc
     5221 gcggtcgcgg attcaggctt ccgcctgggc gcgcttcgcc gcaggttggc gcggtggatc
     5281 gccggcccgc atgacggatg agctccgtaa cgggctattc ctcgtacggt gccagcgtgt
     5341 cggcgacact atgccaggtt gtcgtcaccg tatccggctg gtcccgggtg gacatgactg
     5401 agaactcgca gtaccgatcc ttgaagcgca cccggtcgac atagccgcgc ccgttcgcca
     5461 gcgttacggt gatgctgtcg tcggtctcct tgatctcggc gcccgcgcgg cctgacatct
     5521 gcgacttgag gaggtcccgc gccgagttcc aggcttcgtc cgacccgatc atgttgccaa
     5581 agaccaacca tttatcactc tgcgcggtga caccgcgacc ggtgccgtca tctacccacg
     5641 tgtccgggat cgtgacctga cagactccgt cggtcgcgga gctccccacc gacgcgagcg
     5701 tgccttcggt cacggcggta tccacggcca cgctggtccc cggcacggcc gtggcggtcg
     5761 gctccttctt cccaccaccg ccacacgcgc cgagaaggac ggcggggagc agcagggcag
     5821 ccaggcccag gttcagccgg gctcgaatca tgagatgcga tcgccaaagt gccgcaactt
     5881 gagtgcgacg atcttttcga cgcggccgtt gatatcctgg cgatcgacca ggccattgct
     5941 gatcatgtcc atgacgtggt cgatcagcgc gtcccagccg accggaggcg agccgtaaag
     6001 catgacatcg atcccggcat cgatcgcctg gctgatgatg tcgagcggtt tccagtcatc
     6061 gagcgcgccc atgccgaggt cgtcggtcac gatcacgccg ttgaagccga ggtccttgcg
     6121 taacgcccgg accgcgacct tggagatcga catcggcttg taatcgatcc ggtcatagcg
     6181 caagtggccg agcataacca tatcgacctg cgccttgatc gccgattcga agggaatcgc
     6241 atcggtcttg agccactcgt cccacggcac gtcgatctcg gggatcagct tgtgggagtc
     6301 gacctgcgtg cggccatgac cggggaagtg cttggccgcg ccgatcacgc caccagcacg
     6361 ggaacccttg accgtcgccg agaccttctc ggcaaccgtc ttcgggtcgt ttccgaaact
     6421 gcgtgctgcc atcgtgctgg atgcatcgta cgcaacatcg gccaccggcg cgaagttgac
     6481 gtcgaatccg tatgccgcga ggaaggccgc gcgctccttg ttcagcgtcc gcacgtccga
     6541 gttggagagc gcgcccatct ggaccgcgcc gggagcagga tcaccggcca ggcgtgtgac
     6601 cggcccgccc tcctggtcga cacagatcag cggcggaatg actgggttgg aggcgtggat
     6661 gccgttgatg tacgtggtca gagcgggcgc cgtgctgatg ttgttggcca cgaacaggat
     6721 gccgcccggc atcacccgcg tcaggtcgga cgagtaggtc gacgtcatgt aggtgccgcg
     6781 cgcctcgaaa aagaacatct gggccacgcg atctcgcagc ggcatggtct tgagaatatc
     6841 ccgcgccatc atccgggccg acaccatcga cggtcgccca agcgcaatcg aaccggtcat
     6901 tgccgccgcc gcagccagaa accggcgacg ggacacttct tcacgcactc tcaacgcaaa
     6961 aggtcctgca acgaacgcgt cccacgctca actcagcgca cctggccgca taatcacggg
     7021 ggcagcgcgt gcggggccag ccagatcacc agccatttcg gtgattgccg tacggacagt
     7081 atacccgcgt gcgacacgag ttttggcaac aaagtgcggg aatttcggcc gatttccggc
     7141 aaattcctcc gtatgtcagg agatgcaatg gcttcgtcac ggaacgtccg ggtcggcttg
     7201 gcaggtttgg gtaactttgg ccgcctccac gcccggatcc tctcccagct cggctccgtc
     7261 gacctcgcag ccgtctgcga tcctgactca gacgcgcgaa cctgggcggc tgaaacgctc
     7321 ggcgcgacaa cgacggtggg cgatttcgat gatctgctcc gtgtgcctga actggatgcc
     7381 ctgttcatcg tcacgccgga ggatctacat tatgggcacg tcctgaaagc actcacgctc
     7441 ggtatcccga tcttcatgga gaagccactg agcacgaacg ccgccgatgc gcgaagggtc
     7501 gtcgacgcgg ccagggcagc cggcacattc ctgcagatcg gcttcgtggt ccggttcgac
     7561 gcccagcacg cgatgctccg caaccggatc gtggccggcg agctgggatc gatcgtcctg
     7621 ttccgagcca agcgaaactg ctcgcggagc tggtttgcga cctacggaga tcgcgcccac
     7681 acggtttacg agtccatcat tcacgacatc gacctcgccc tctggttcac ccaatgccgc
     7741 tgcacgaagg tctatgcggt gcagcgaaac atctctggcc tcacttatcc cgatgccctg
     7801 gccgcgatgc ttcagctcga gaacggatcc gtggtaatgc tcgacaccag ctggttcgtg
     7861 cccgaccagg cgccgcaaaa cgtgatcgcc ggcgactgga ccggcacgat cgatgccgag
     7921 ttcgagctga tcggcaccga gcaaactgcc cgctatcgcc tgctcgattc cggcatgtcg
     7981 atcgcgacgc ctgatcagat catccatccc gaggtcgggc tctggccgga ggtctatggc
     8041 tcgattggcg gcgcgctccg actcgaggat gagcacttca tctcgtgcgt ccgctcgggc
     8101 cgaccgtcaa ccatcgcatc gctcgacgac gccctccacg gcctcgaaat cgccgaagca
     8161 atcgtccaca gcgccgaatc cggactcgaa gtcacgctgt aggaccccac gctacgtcga
     8221 caaaccggtc aatctggtcg cggggcgctt tacggctcgc ggagccaggc gaggaggagt
     8281 tgggcagtcg cctcgagtga atcccggtgg aggcgctcgt acgcgtggga cgagtcgacg
     8341 cccggcccaa ccaacgcacc ccggatgtca tatccagccc gcagggc
//
LOCUS       DATAOZ010000664         3646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_5254207, whole genome shotgun sequence.
ACCESSION   DATAOZ010000664 DATAOZ010000000
VERSION     DATAOZ010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5254207"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VHR64_16680"
     CDS             complement(<1..1219)
                     /locus_tag="VHR64_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX3304524.1"
                     /translation="MVLRDRGFQRRALARVGSPAVAETNQYDYGPAASTPFPGSTGPN
                     HQAGDVAAIADRPRTLMSREVVELKHKLHDRMVREIDPSRMTADLSPDDARRAVEDAV
                     LELLAQEDVSLSRTDELTLMHDIADEIVGFGPIEPLLQDESITEVMINRPDRVYYERE
                     GVLHLSDRSFRDEDHIVRLIDKIVSPLGRRIDESSPMVDARLPDGSRVNAIIPPLAIQ
                     GPSLTIRKFAKRAYSDQDLVRFGTMSQAMCELLRACVWSRLNVLVSGGTGTGKTTFLN
                     VLSSYISNRERIVSIEDPAELRLQQDNWVSLETRPPNIEGRGQVVQRDLVRNALRMRP
                     DRIIVGECRAGEAFDMLQAMNTGHDGSLTTVHANSPRDALARIENMVLMANLDLPVRA
                     IREQIASAINLVIQ"
     gene            complement(1225..2676)
                     /locus_tag="VHR64_16685"
     CDS             complement(1225..2676)
                     /locus_tag="VHR64_16685"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX3304525.1"
                     /translation="MTTPSTKVLIVDDSEKTRDTLIELLRFDDIEVVGESTFGAAAYT
                     WADRLDVDVVVMTIEEPVARSLRTVEAMMSGARTWPVVGVSSRGDRETMRKAMVSGVR
                     DYVVMPMPPEELRATIINVHHIERARRAAVQQGQVSRPLGTVITVAGFKGGIGKSTIS
                     SNVAVALAQQTRQHVALIDLDLQFGDAAVMLDVVPTTTIEHVAKEMERLDPQLLQGYL
                     ATHASRLKLLAAPTTPEASDAITDEGASRILEMLAETNDFVVVDTAPHLDGLSVAAMD
                     LSTFVLIVVVPEIPCLRRTKAALALMQSWGYSRDKVKLVVNRSHRRGSVTTQEIEQVL
                     NYPVYAEVPDDRSVGKSVAIGTPVAMSAPKSRAGRAANDLARTLTGVPRPTERISILR
                     RRPTNGTLPQVRPVLSAAPPVNGTAQHFGNGASDSAIPWEYWPSESLGSGHGTGTGLP
                     ESNGNGHRPDVPIREIVHAGESAGWSPLTAAEE"
     gene            complement(2711..>3646)
                     /locus_tag="VHR64_16690"
     CDS             complement(2711..>3646)
                     /locus_tag="VHR64_16690"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3304526.1"
                     /translation="TPTPVGTSEATDEAQPVARPDAKSVTLAVTPEEAQRLLLAAQTV
                     KNEKGSLRLAMRAPGDTTTVDLDAAQLGNIPLGELLGDVNQPMVPTDVVITQATFTQR
                     VLASGTVLEFKATVKNISDRTIKSGSDAPPEYEYTQGVAYDALGFFPKANTYRIGLSF
                     AGAYPNQYPYRWALGQDLEPGQSVDVVGSVRLTEPTAATKYWLGIIQEPNVVTQDGVN
                     VSDITVVPSESGTVSTKKAELRSDPSASAAVVVELEKGADLEIVQARGAWFQVRSGKS
                     EGWIEASSVTVPPLGATDSGTASSSDTSQSADSNT"
BASE COUNT          608 a         1214 c         1158 g          666 t
ORIGIN      
        1 gttggatgac gaggttgatt gccgaggcga tctgctcgcg aatggcgcga actggcaaat
       61 cgaggttggc catcagcacc atgttctcga tccgggccag agcgtcgcgc ggtgaattgg
      121 catggaccgt ggtcaacgac ccgtcgtgac cggtgttcat cgcctggagc atgtcgaatg
      181 cctcgccggc acgacactcg ccaacgatga tccggtccgg ccgcatgcga agggcgttcc
      241 tgaccaggtc ccgctggacg acctggccgc gaccctcgat attcggcggg cgcgtctcca
      301 ggctgaccca gttgtcctgc tgtaaccgca gctcggccgg gtcctcgatg ctgacaatcc
      361 gctcccggtt actgatatag gaagagagca cgttcaggaa ggtcgtcttg ccggtaccgg
      421 tgccacccga gaccagcacg ttcagccggc tccagacgca cgcccgcagc agctcgcaca
      481 tcgcctggct catcgtcccg aaccggacga gatcctgatc ggaataagcc cgcttggcga
      541 acttccggat cgtcagcgaa ggaccctgga tagccagtgg cgggatgatc gcattgacac
      601 gggaaccgtc gggcaggcga gcgtcgacca tcggcgacga ctcgtcaatg cgccgcccga
      661 gtggcgagac gatcttgtcg attagccgca cgatgtggtc ctcgtcccga aacgagcgat
      721 cggagagatg cagcacgcct tcccgctcgt aatagacccg gtcgggccga ttgatcatga
      781 cttcagtaat gctctcatcc tgaagcagag gctcgatcgg cccgaacccg acgatctcgt
      841 ctgcgatgtc gtgcatcagc gtcaactcgt cggtgcggga gaggctgacg tcctcctgcg
      901 cgagtagctc aagcacggca tcttcgacgg cccgcctggc atcgtccggg ctgagatccg
      961 ctgtcatgcg cgacggatcg atctcgcgaa ccatccggtc gtggagcttg tgcttgagct
     1021 cgacgacctc ccggctcatc agggtgcgtg gacgatccgc aatcgccgct acgtcgccgg
     1081 cctgatgatt gggccccgtc gatcctggaa acggcgtaga tgcggccggg ccgtagtcat
     1141 actgattcgt ctcagcaacg gccggactcc cgacccttgc cagcgcccgg cgctggaaac
     1201 cgcgatcacg caggaccatg gcccctactc ctcagccgcg gtcagcggcg accaaccggc
     1261 cgattcgccg gcgtggacga tctcacgaat cgggacatcc ggccggtgac cgtttccgtt
     1321 gctctccggg aggccagttc ctgttccgtg gccactgccg agcgattccg acggccagta
     1381 ctcccacggg atcgcggaat cgctcgcccc gttgccgaaa tgctgagcag tcccgttcac
     1441 cggcggcgcg gcggacagca ccgggcgaac ctgcggcaag gtgccgttcg tcggccggcg
     1501 gcgcagaatg ctgatacgct ccgtcggccg cggcacgccg gtcagcgtac gagccagatc
     1561 attggcggcc ctaccggctc gactcttggg cgccgacata gcgaccggtg tcccaattgc
     1621 gactgacttg ccgacgctcc ggtcgtccgg tacctcggca tagaccgggt aattgaggac
     1681 ttgttcgatc tcctgggttg tgacggaacc acggcggtgg ctccggttca cgaccagctt
     1741 caccttgtcc cgcgaatacc cccatgactg catcagcgcg agggccgcct tggtccggcg
     1801 caggcacggg atctccggca cgacgacgat caacacgaat gtcgagagat ccatcgccgc
     1861 gacggacaac ccgtcaagat gcggcgcagt gtcgaccacc acgaagtcgt tcgtctcggc
     1921 caacatctcg aggatccggc tggccccttc gtcggtaatt gcgtcagatg cttccggcgt
     1981 cgtcggcgcg gcgagcagct tgagccggga ggcatgtgtc gccaggtagc cttgcaggag
     2041 ctgcggatcg agccgctcca tctccttggc cacgtgctcg atcgtcgtgg tcggcaccac
     2101 atcgagcata accgcggcat caccgaactg cagatcgaga tcgatcagcg cgacatgctg
     2161 ccgggtttgc tgggcgagcg cgaccgcgac attgctggaa atggtcgact tgccgatgcc
     2221 acccttgaac ccggcgacgg tgatcaccgt gccgagcgga cgactgacct gtccctgctg
     2281 gacggcagcg cggcgggctc gttcgatgtg gtggacgttg atgatcgtgg cgcgcagctc
     2341 ttcaggcggc atcggcatca cgacgtagtc gcgcacaccg gagaccatcg ccttgcgcat
     2401 cgtctcgcga tcgccgcggg aggagacgcc gaccaccggc caggtccggg caccgctcat
     2461 catcgcctcg acggttcgca gcgagcgagc gaccggctcc tcgatcgtca tcacgacgac
     2521 gtcgacatcg agacgatcgg cccaggtgta ggccgcggcg ccgaaggtgc tctcgccgac
     2581 gacttcgata tcgtcgaatc gcagcagctc gatcagcgta tcgcgcgtct tctcgctgtc
     2641 gtcaacgatc aggactttgg tgctcggcgt agtcatcgga cgatccccca ggtacagcgc
     2701 gtttgcctgc tcatgtattg ctgtccgcgc tctgcgatgt gtcggacgag ctcgcggtgc
     2761 cgctgtccgt cgcgccgagc ggcgggacgg tcacggacga ggcctcgatc cagccctccg
     2821 atttgcccga gcggacctgg aaccaggcgc cgcgggcctg aacgatctcg agatccgcgc
     2881 ccttctccag ctcgaccacg actgctgccg atgcgctcgg atctgatcgc agttcggcct
     2941 tcttcgtgct gacggttccg gactcggatg gcacgaccgt gatgtcggag acgttgaccc
     3001 cgtcctgcgt gaccacgttc ggttcctgga tgatgccaag ccagtatttg gttgcggccg
     3061 tcggctccgt cagccggacc gagccgacca catcgaccga ctgccccggc tccaggtcct
     3121 gtccgagcgc ccaccggtac ggatactgat tcgggtaggc gccggcaaag ctcagcccaa
     3181 tccggtaagt gtttgccttc gggaagaagc caagcgcgtc gtacgccacg ccctgcgtgt
     3241 actcgtactc cggcggcgca tccgacccgc tcttgatcgt gcgatccgag atgttcttaa
     3301 ccgtcgcctt gaactcgagc accgtcccgg aggcgagcac ccgctgggta aacgtcgctt
     3361 gggtgatcac gacatcggtc ggcaccatcg gttggttgac atcgccgagg agctcgccga
     3421 gcgggatatt gccgagctgg gcggcatcga gatcgaccgt ggttgtatcg cccggcgccc
     3481 gcatcgcgag gcggaggctg cccttctcgt tcttgaccgt ctgcgcggcc agcaggaggc
     3541 gctgcgcctc ctcgggggtc acggccagcg tcacgctctt ggcatccggc cgtgcgaccg
     3601 gttgcgcctc gtcagtcgcc tccgacgtac cgaccggcgt cggtgt
//
LOCUS       DATAOZ010000665         6076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4993785, whole genome shotgun sequence.
ACCESSION   DATAOZ010000665 DATAOZ010000000
VERSION     DATAOZ010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4993785"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VHR64_16695"
     CDS             <1..1098
                     /locus_tag="VHR64_16695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX3304527.1"
                     /translation="RPTIQEIAPGARIINLDVGPAAPMDKNHLYELVPTFARACVLFS
                     MTEGIKYDVVHAHYWLSGMVAEELRSYWEAPVVLMFHTTADMKNAVLGGGDQESALRQ
                     ETEHTLALLADSLIAANPDERADLIWTMGATDERVCTIPPGVDLSLFRPLDYHESRRE
                     LGWSIKERIVLFVGRIDPIKGIDTLIDTMPMVMEPELHYVLIGGDLDADGKPIGPLAA
                     VKERVEALGMGRRCRYLGAQPQDLLPLYYSASDVVAVPSRYESFGLVAVEAMATGTPV
                     IASSVGGLTFTVEDGRNGLLVPYGNARALAGAIDRLLEDDDFREAMSFHAIEDSRRFS
                     WEAVAEQVLHVYQRLAEGYRADLCCAEEIYA"
     gene            complement(1158..2435)
                     /locus_tag="VHR64_16700"
     CDS             complement(1158..2435)
                     /locus_tag="VHR64_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX3304528.1"
                     /translation="MVEAALDPKQLIPRVVGRYSPVTVDHGEGIYVWDTNGERWTDFT
                     SGIAVTNTGHCHPRVVAAIQEQAGKIIHAQQNIFAHEPMMKAAAALTETLPARLNQVF
                     WANSGAEAVEGAIKLAKVATGRPAIIAFRGAFHGRTHAAMSVTSSRSKVRGHYEPLLA
                     GIYYAPYPYLFRNPYKGNPEDADLAYFSELEYLFETMVMPDDVAGIIVETIAGEGGYM
                     VPTERWFKKVRELCDKYGIMLIMDEIQSGVGRTGKMWGFEHFGIEPDIMTVAKGIASG
                     MPVSAIVSDKAIMDKWAPGAHGGTYGGNAVGTAAAYATLQVMKDEKLVENSAAMGAVL
                     TSGLEEIQARNPVIGDIRGKGLMVATEFVKPDGAPNPEAVAKVIQKCQDEKVLLLNCG
                     TFDQAIRIIPPLVVNETQINEFLDVYERAVTSL"
     gene            complement(2472..2816)
                     /locus_tag="VHR64_16705"
     CDS             complement(2472..2816)
                     /locus_tag="VHR64_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304529.1"
                     /translation="MNVIEGLRTIRQRLVDNGANDATLTHVDQIIKRASVPSVGSASA
                     QSLLQLTRMLMRHPTASSNSAVYNDLALLEEQLESAAVEGARRAAEDAEKPMPKSKKF
                     YKELKEKQKKTS"
     regulatory      2925..3032
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3023..3919
                     /gene="thiD"
                     /locus_tag="VHR64_16710"
     CDS             3023..3919
                     /gene="thiD"
                     /locus_tag="VHR64_16710"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX3304530.1"
                     /translation="MHYPDMTINQAGIGTAANNRVPRAMTIAGSDSGAGAGIQADLKT
                     FAAHGVYGTSVLTAVTAQNTTSVLAIAEVPEEVIVHQIDGVMEDIGADAIKTGMLSAA
                     SIIEVVADRIEAWGVKNLVVDPVMVAKSGDALLQPTAVRTMVKLLLPLALVVTPNIPE
                     AEVLSGKRIVNQASEEDAARIIAGLGPRYVVIKGGHRDSGDAMDLIYDGESFERITSP
                     RIETANTHGTGCTFSAAIAANLALGAEPSAAITRAKEYLSGALRESFPIGAGHSPVNH
                     FHAVPQIDPRTLAVGSGGTHGS"
     gene            3909..4691
                     /locus_tag="VHR64_16715"
     CDS             3909..4691
                     /locus_tag="VHR64_16715"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX3304531.1"
                     /translation="MARDDSPLVVAGTPLRSRLFVGTGKYRTSEEMNAAFAASGTELI
                     TVALRRIDFDDPKSRSVLEDVDWKRYRILPNTAGCQTAEDAIRIARMARAMELSDWIK
                     LEVIPDPKYLLPDPIGTLDAARVLIDEGFTVLPYIGADPILAKRLQEMGTATVMPLGS
                     PIGSGQGILNLEAIKIIIEQATVPVVVDAGIGTPSDAAIAMEAGADAVLINTAIALAE
                     DPALMAEAMAQAVDAGRKAYLAGRIPVKPYASASSPTTNLVA"
     gene            4684..5313
                     /gene="thiE"
                     /locus_tag="VHR64_16720"
     CDS             4684..5313
                     /gene="thiE"
                     /locus_tag="VHR64_16720"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX3304532.1"
                     /translation="MPELPPQLRLYLVADPDHTSGDFVAMIEAALAGGVTSVQLRCKG
                     RTDREVMNLARAIRAATHKHGALFLVNDRVDIALACGADGVHLGVDDLPIEDARQLGG
                     STFVVGFSPETDEQAQSARSRGADYLGVGPVFGTSTKSDAGAAIGLETISRRAGLAGV
                     PIIGIGGITAANARSVIDAGAVGVAVVSAISMHGDPKRAAQSLGRSIER"
     gene            5310..5918
                     /locus_tag="VHR64_16725"
     CDS             5310..5918
                     /locus_tag="VHR64_16725"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX3304533.1"
                     /translation="MTISAIPRLVVILDRAGARNDVVEVAVAAIRGGADVIQIREKAM
                     PEAEVSKIVARVTEMVGDPLRVAVNGFPEIAARLGTHLHLPEAMALDRDDVRHAPDAL
                     LSRSIHGPAPAVSADYAILGNLLETGSKPGKAGLGFERFEELARACPAPVLAIGGIEP
                     GNVRDVLRHGGHGVAVRSYVIGAAHPERAAREIREELDFWKR"
BASE COUNT         1083 a         1870 c         1992 g         1131 t
ORIGIN      
        1 cgaccgacga tccaggagat tgcgccgggc gcccgcatca tcaatcttga tgtcggtccg
       61 gccgcgccga tggacaagaa tcacctctac gagctcgtcc cgaccttcgc gcgtgcgtgc
      121 gtcctcttct cgatgactga aggcatcaag tacgacgtcg tccacgcgca ctactggctc
      181 tccgggatgg ttgcggagga actcagatct tactgggaag cgccggtcgt gctgatgttc
      241 cataccacgg ccgacatgaa gaacgcggtg ctcggcggcg gcgaccagga atcggcgctg
      301 cgccaggaga cggagcacac actcgcactc ctggccgata gcctgatcgc ggccaatccg
      361 gatgagcgag cggacctgat ctggaccatg ggcgcgaccg atgagcgggt ctgcacgatc
      421 ccgcccgggg tcgatctcag cctgttccgt ccgctcgact atcacgagag ccggcgcgag
      481 ctgggttggt cgatcaagga gcgaatcgtc ctctttgtcg gccggatcga tccgatcaag
      541 ggtatcgaca cgctgatcga cacgatgcca atggtgatgg agccggagct gcactacgtc
      601 ctgatcggcg gcgacctcga cgccgacggc aaaccgatcg gaccactcgc cgcagtcaag
      661 gaacgagtcg aggcgctggg gatgggccgg cgctgtcgct accttggcgc gcagccgcag
      721 gatttgctgc ctctctacta ctcggcgtcg gacgtggtcg ccgtgccgtc gcggtacgag
      781 tcgttcgggc tcgtcgcggt cgaggcgatg gcgaccggca cgccggtgat cgcatcgagt
      841 gtcggtggcc tgacgttcac ggtcgaggat gggcgcaacg ggcttctggt tccgtatggg
      901 aatgctcgcg cgctggccgg tgcgatcgac cgcctgctcg aggacgacga cttccgtgaa
      961 gcgatgagct ttcacgcaat cgaggactca cggcgcttct cctgggaggc cgtcgccgag
     1021 caggtcctgc acgtctatca acggctggca gagggatatc gcgccgatct ctgctgcgcc
     1081 gaagagattt acgcctgaaa tggcgagagg gcggcgaatg tgccgccctc tcctgcaatt
     1141 ccgggacggc ctgatgtcta cagcgacgtc accgcacgct cgtagacatc gaggaactca
     1201 ttgatctgcg tctcgttgac gaccagcggc ggaatgatcc ggattgcctg gtcgaaggtg
     1261 ccgcagttga gcagcagcac cttctcgtcc tgacatttct ggatgacctt cgccaccgct
     1321 tcggggttcg gcgcgccatc cggcttgacg aactcggtgg cgaccatcag ccccttgcca
     1381 cggatgtcgc cgatcaccgg gttgcgagcc tgaatctcct cgagcccgga tgtcagcacg
     1441 gcgcccatcg cagctgagtt ctcgaccagc ttctcgtcct tcatgacctg gagcgtggcg
     1501 taggccgcgg ccgtaccgac agcgttgccg ccgtaggtcc cgccgtgcgc gccgggcgcc
     1561 catttgtcca tgatcgcctt gtccgagacg atggcgctga ccggcatgcc cgaggcaatg
     1621 cccttggcaa ccgtcataat gtccggctcg atgccgaaat gctcgaagcc ccacatcttt
     1681 ccggtgcgac cgacgccgga ctggatctca tccatgatca gcatgatgcc gtacttgtcg
     1741 cagagctcgc ggaccttctt gaaccagcgc tcggtcggga ccatgtaccc gccctcaccg
     1801 gcgatcgtct cgacgatgat cccggcgacg tcatctggca tcaccatggt ctcgaagagg
     1861 tattcgagct cgctgaaata cgcgaggtcg gcatcttcag ggttgccctt gtacggattg
     1921 cggaagaggt acgggtacgg cgcgtaatag attccggcca ggagcggctc gtaatgcccg
     1981 cgcaccttcg accgggacga ggtcaccgac attgcggcat gggtgcggcc gtggaacgcg
     2041 ccgcgaaacg cgatgatcgc cggccggccc gtcgctacct tggccagctt gatcgcgccc
     2101 tcgacggcct cagcgccgct attcgcccag aagacctggt tgagccgtgc gggcagtgtc
     2161 tcggtcaggg ctgcggcggc cttcatcatc ggctcgtgcg caaagatgtt ctgctgcgca
     2221 tggatgatct tgccggcctg ctcctggatt gcggcgacga ctcgcgggtg gcagtgacca
     2281 gtgttggtga ccgcgatgcc agacgtgaag tcggtccacc gttcgccgtt tgtgtcccag
     2341 acgtagatgc cctcgccgtg gtcgacggtg accggtgaat accgaccgac gacgcgggga
     2401 atcagttgtt ttggatcgag cgctgcttca accacgatgt cctcctcagg aagcttggcg
     2461 tttgacaatg actacgatgt cttcttctgc ttttccttga gctctttgta gaacttctta
     2521 ctcttcggca tcggcttttc agcatcttcc gccgcccgac gcgcgccctc gaccgcggcc
     2581 gattcgagct gctcctctag cagggccagg tcgttataga cagccgagtt ggacgacgcc
     2641 gtggggtgcc gcatcagcat tctggtcaac tggagcaacg actgcgccga agcgctcccg
     2701 actgacggaa cagaggcgcg tttgatgatc tggtccacgt gggtgagcgt ggcgtcgttc
     2761 gccccgttgt cgacaaggcg ttggcggatc gtccgcaggc cctcgatcac gttcatggtg
     2821 gacatccttt atgagcggct cggtacgcgt tgggaattct agcctacgag accgcccaat
     2881 cgattcgatt ctccgcgccg attcccgaca tcgatggtaa aattgaggcg tgccaggggt
     2941 gcgttgtgga cgctgagagc gcggcgcaaa gccgccaacc ctcggaacct gatccggctc
     3001 gtaccggcgt aggaaggcgc tgttgcatta cccagatatg acaattaacc aggcaggaat
     3061 cgggaccgct gcgaacaatc gcgtcccgcg cgccatgacc atcgccgggt ccgattccgg
     3121 cgccggcgcg ggcatccagg cagatctcaa gacattcgcc gcgcacggcg tctacgggac
     3181 gtcggtgctg accgcagtca cggcgcagaa cacgacgtcg gtcctcgcga tcgccgaggt
     3241 gccggaggag gtcatcgtcc accagatcga cggcgtgatg gaagacatcg gcgccgatgc
     3301 gatcaagacc ggcatgctct cggccgcgtc gatcattgag gtcgtcgcgg accggatcga
     3361 ggcatggggc gtcaagaatc tcgtcgtcga tccggttatg gtcgcgaaga gtggtgacgc
     3421 cttgctgcag ccgacggctg tgcgaacgat ggtcaaactg ctcttgccgc tcgcgctggt
     3481 cgtaacgccc aatattccgg aggccgaggt gctgagcggc aaacggatcg tcaatcaggc
     3541 gtccgaagag gatgcagcgc gaattatcgc gggtctcggt cccaggtatg tggtgatcaa
     3601 gggcggacac cgggattcgg gcgacgcgat ggatctgatc tacgacggcg agtcattcga
     3661 gcggatcacc agcccacgca tcgagaccgc caacacgcac ggcaccggct gcaccttctc
     3721 ggccgcgatc gccgccaacc ttgcactcgg agctgaacca agcgcggcga tcacccgcgc
     3781 caaggaatac ctttcaggcg cgttgcggga gtcgttcccg atcggcgccg gtcattcacc
     3841 cgtcaaccat ttccacgccg tcccgcagat cgatccgcgc acgttggcgg tcggatcagg
     3901 aggaacccat ggctcgtgat gattccccac tcgttgttgc cggcacgccg ctgcgcagtc
     3961 gcctgttcgt tggcaccggc aaataccgga cctcggagga gatgaacgcc gccttcgcgg
     4021 catccggtac tgaattgata accgtggccc tgcgtcgaat cgacttcgac gacccgaaga
     4081 gccggtcggt gctcgaggat gtggactgga agcgatatcg gatcctgccg aataccgccg
     4141 gctgccagac ggccgaggac gcaatccgaa tcgcgcggat ggcacgagca atggagttgt
     4201 cggactggat caagctcgag gtaatcccgg atcccaagta tctgctgccg gatcctatcg
     4261 gtacgctcga cgcagcccgg gttttgatcg acgaagggtt cacagtcctg ccctatattg
     4321 gtgcggaccc catcttggcc aagcgattgc aggagatggg cacggcgacg gtcatgccgc
     4381 tcggctcgcc gatcggttcg gggcaaggaa tcctgaacct cgaggcaatc aagatcatca
     4441 tcgagcaggc gacggtgcca gtcgtggtcg acgccggaat tggcacgccg agcgatgccg
     4501 cgatcgctat ggaggccggg gcggacgctg ttctcatcaa cacggcgatt gcgcttgctg
     4561 aagatccggc cctgatggcc gaagccatgg cgcaggcagt ggacgccggg cgcaaggcgt
     4621 acctggcggg acgaatcccg gtcaagccgt atgcctcggc cagcagcccg accacgaacc
     4681 tcgttgcctg agcttccgcc gcaactccgg ctgtacctgg tcgccgatcc ggatcacacg
     4741 agcggcgatt tcgtcgcgat gatcgaggcg gcgctggccg gcggagtgac ctccgtccag
     4801 ctcaggtgca aaggtcgcac tgatcgcgaa gtcatgaatc tcgctcgggc gatacgcgca
     4861 gcgacccaca aacacggggc gctcttcctc gtgaacgatc gcgtcgacat cgcgctcgcc
     4921 tgtggggcag acggcgtgca cctcggcgtc gatgatctgc cgatcgaaga tgcgcgacag
     4981 ctcggcggat caacgttcgt cgtcggcttc tcgcccgaga ccgacgaaca agcgcagtcg
     5041 gcaaggtccc ggggtgcgga ctacctcggt gtgggaccgg tctttggcac atcgacgaag
     5101 tcggacgcgg gtgcggcgat tggcctggag acaatatcgc gacgcgcggg tctggccgga
     5161 gtgccgatca tcggcatcgg cggaatcact gcggccaatg cgcgctcggt gatcgatgcg
     5221 ggagcggtcg gcgtcgcggt cgtctcggcc atttcgatgc acggtgaccc gaagcgcgcc
     5281 gctcagtcac ttgggcgctc gatcgaacga tgacgatatc ggcgatcccg cggctcgtcg
     5341 tgatcctgga tcgggccggc gcgcgaaatg acgtcgttga ggttgccgtc gccgcgatcc
     5401 ggggcggagc cgacgtcatt cagattcgtg agaaggcgat gcccgaagct gaggtgtcga
     5461 agatcgtcgc tcgggtcacc gaaatggttg gtgatccgct gcgtgttgcc gtcaatggct
     5521 tcccggagat tgcggccagg ctgggcacgc acctgcacct tccggaggca atggcgcttg
     5581 accgggacga cgtccgtcac gctccggatg ccctgctgag ccggtcgata cacggtccgg
     5641 caccggcagt cagtgcggac tacgcgatcc ttggcaacct gctcgaaacg ggaagcaagc
     5701 cgggcaaggc gggcctggga ttcgagcgtt tcgaggagtt ggctcgcgca tgcccggcac
     5761 ccgtcctggc gatcggtgga atcgaaccgg gcaatgtcag ggatgtcctg cggcacgggg
     5821 gccatggcgt cgcggtccgc tcgtacgtga tcggcgcggc tcatccggaa cgggcagcac
     5881 gcgaaataag ggaggagttg gatttttgga agagatgatg gtgccggagc gcactgtgat
     5941 tagcgtcacg ttgaatggga aaccgcgaga ggtcgcgtcc gatcaaacgg tggctggcct
     6001 cctggaagag ctcgacttgc gggagcggct ggtcgtcgtt gagcgaaacg gctcgatcgt
     6061 gaaccgaacg gatttc
//
LOCUS       DATAOZ010000666        14071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7363225, whole genome shotgun sequence.
ACCESSION   DATAOZ010000666 DATAOZ010000000
VERSION     DATAOZ010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14071
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7363225"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2275
                     /locus_tag="VHR64_16730"
     CDS             <1..2275
                     /locus_tag="VHR64_16730"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX3304534.1"
                     /translation="ISPDWTDSVDTTAIDPSFTNIESAVALARSLPVGGNRRIVLVSD
                     GSENLGSVQNQVAQAARDGVPIDVVKVAGAGNDDLRVDSVTAPAAIWQGEKPNVLVSV
                     STVTAGKAHLELVVDNGVVATQDVDLSAGLSTFAFAVPELGPGFHSMSVRVSGDPTMD
                     QFAENNAAPLALIVRDAPHVLLVAVAGSDPSRLVNALKNRGAEVTNVAPEQVPVQLSV
                     LGTYDAFVLDNVPATALQVEQIAGLQEATRTLGKGLVVIGGTSSYGPGQYASTRLEDM
                     LPVTVKVTDGRERQHVALLLVFDHSGSMSYDPLHETSKIDMAKEAIRLAANALSDGDT
                     IGVLAFSDSQDWVFPLTQITGDSTRQQLNAAVSGVKASGGTEIYPALQVGLDAIRNVD
                     ADVRHVVLLSDGKSKSGTQDAYVKLVSDAGADRTTVSTIAIGNDADTGLLQAIAQAGG
                     GRYHFTNRAEEIPQLTLEEARSAGAQSVIRGAFQAVQTLPSPIMTGFDVKTLPPLDGY
                     DFTEARPGAQVILVSHRNDPVLAKWQYGLGRVVAWTPDDGADLASQWGSWQNFDQFWS
                     SVLRWTLPDPENRAITTDVARDGPDVVLSLSTTGDASGNDYVDLSAMTARITGPDGAV
                     TGSIPLTQSGSGQFQIRIRNADSGAYKLELLDGSGTAQGGDYGFTLPGSPELLPSPGG
                     DRLLASIAAATGGRELSMDEPGDLFSAPSSSGETLQTYRPIWSWAVLGALGLFLLELM
                     LRLNGYARLRSLRGRLG"
     gene            2334..3308
                     /gene="corA"
                     /locus_tag="VHR64_16735"
     CDS             2334..3308
                     /gene="corA"
                     /locus_tag="VHR64_16735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX3304535.1"
                     /translation="MIKVKVHAEGAVLDQDLPLDRISDTLQSDNSLLWVDVIAPNAKD
                     IELLEQEFGFHHLALEDAALVHERPKIDLYDDFLLIIFYALTHERRETPGLRLEQLSL
                     FAGKNFVVTLHQAELPVLDQTSDRWCASHAKIKNGAAGLLVYSILDALVDEYLPIADR
                     ISDRIDVIEDAVFGRFDANAQQEIFRIKRNLLQMRKVLTPERDVLNVLMRRDTPIFSD
                     ETVRYFQDIYDHLLRVLDSIDTYRDLLSSALDSYLSVQSNRMNKVMKTLTASSIILMT
                     VTLVASIYGMNFVHMPELKWHYGYALALGLMVTIAATLGALFRKIDWF"
     gene            complement(3485..4879)
                     /locus_tag="VHR64_16740"
     CDS             complement(3485..4879)
                     /locus_tag="VHR64_16740"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX3304536.1"
                     /translation="MNEHVRQIVMPEPRRWRLAPDAWRLAGRVTPGRLERYLRFRSAK
                     GMPRSMKDRFRAMRIPEQTISEVLSGIHGLGEWMDGWNLAAQRFLTEARGEERSGRWQ
                     EAAVARINAAMCFHIAHLVTDTDPRTLRTLRASSVTTFAQAVPRLMPEVRKISLRWRA
                     RTLPAYAAVPQSGQRPFPMLVLLNGATTCKEELLLWALPLLDMGIAVMTIDWPGTGES
                     ASFTSPIADCDDMTDGIFDFVEDHPDLEPDMVAIAGFSLGGPVAIRCAAYDRRLLGAI
                     AVTPPYDPLDWWSHVNPLVRLQLMTLAVENDTAEDVVAEFGLVDLIPRLRSPLLVFGA
                     GRDMVVPPEEAVALAAAAGDLATLVWYPEAGHGLYSELEDWISLSGEWLNELVGRDLP
                     AEQAPLPEPEIGSTAVRIEPHSEQVRPVPPEDVVPMSTRVDVRVPDRQPVAVRSEPDN
                     EVDEDEELPDLWDE"
     gene            complement(4932..5522)
                     /locus_tag="VHR64_16745"
     CDS             complement(4932..5522)
                     /locus_tag="VHR64_16745"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX3304537.1"
                     /translation="MTSQHLPREEGIRFGRTLISVAVGELVDQPVEAIVAAGNQRAMF
                     AAGPSSALWSAAGEAVERELRSHAPLEIGNAVATGSGRLADRGIQHILHVIIATELGE
                     PPKRLEIPHALREALDLATKLRVRSIALPMLGTTAGAPRDDRLDAARVVIETFVAQLR
                     TRPHRIERGILVSRFEDDRAPLQELLTRARERLWTE"
     gene            complement(5599..7821)
                     /locus_tag="VHR64_16750"
     CDS             complement(5599..7821)
                     /locus_tag="VHR64_16750"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX3304538.1"
                     /translation="MIGHSRGHGSAAHPLSHSEAEALISARLDAPLTPQQEQRLASHL
                     ATCASCRQFADQMYAMSAGIRSLPRLPASPIVSRQVRERIAQPQSIWERIGGLMGGRV
                     GFAPMAATAAMLALVIAGYALFQGNDDNGRLGPTITAGTVVAQNATQTSDASQVTQTS
                     SSAITPGVEMPTETPVNVPNVRASDETPTETPTTAVLAAEAPTSTATETDTPTTVPTE
                     TSVPTDTPTETSVPTDTPEPTATRTPKPEPTETPAPTETTVPTEVPTATETATTEPTV
                     TKTATAEPTATNTATAEPTATRTPTKEPTATRTPTEEPTATETREPTETATPEPTETP
                     KPKRPTRTPTAEPTATEEPAQQPTIGPSDNTAPTEAPPPSTEVPTETPVEEPTDSTQI
                     VPVDGTGAAGETPTSEATAETTAIGGPTEEPNGDQTSNTAALLDDTARIAGISSGGAP
                     LGPLRVNSPGTLMVLSSDAGGSDLQVVSMSDGSVVAHLGAGADPIWSPLGIVLLYQSY
                     ADGTPSVGLYDGDTGKFEPISDPSKDGAVQDIPAGWSGTSAYYLRETGDQDSTVILYG
                     YDVNSGETTEVWRSTGVALSGGRPIPTGDGFLIATTASWLLVGADGSESNLGPNDFGL
                     TGEGFLSPGANLVAYPAGDQLIVADASSPGIPMAQIPYVNGNGAGFTWSPDGSYLAVS
                     DGYSLQIYDTSGTFISAATSDNGVVIAAPQWLSDGIYYVETSPTPSLRRLLESKIPGI
                     "
     gene            complement(7818..8405)
                     /locus_tag="VHR64_16755"
     CDS             complement(7818..8405)
                     /locus_tag="VHR64_16755"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX3304539.1"
                     /translation="MTDEVELVARARSGDPVAFERLFERYHAPVLNYLHRMVGDRALA
                     EDLTQDAFVKAYRALPNTRPDLAFKAWLYRIATNTAISHLRRRKLIRWVPFLGTQDYA
                     SNESIERSVGRKHDVEQALRKLPEHYAAVLILRHYEGLSLAETAAALDITENAAKLRL
                     FRARKAFANVYTDPVQEELAADMETARIDNSEGAR"
     gene            8740..9144
                     /locus_tag="VHR64_16760"
     CDS             8740..9144
                     /locus_tag="VHR64_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304540.1"
                     /translation="MADADAPAGRSNYGDTFAEALERFLNYLADFGLSPDHCVESRMA
                     RFQSEATTLPELARNAFEAVVALAAEFELTVVRVSVDGHRPIEGGHRCWGTVELARYA
                     ESSGPVRIVDGPVVSRDPDDGQWRIDVWFVDD"
     gene            9242..9436
                     /locus_tag="VHR64_16765"
     CDS             9242..9436
                     /locus_tag="VHR64_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304541.1"
                     /translation="MNGQALVYFGISGATLLLALSLLRKRYVEQDHSAVTSRGGYTMF
                     AILMLIFSIGAFFAGYFSRS"
     gene            9445..10233
                     /locus_tag="VHR64_16770"
     CDS             9445..10233
                     /locus_tag="VHR64_16770"
                     /inference="COORDINATES: protein motif:HMM:NF015402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HEX3304542.1"
                     /translation="MPEPRDWPRIVGVLHLPPLPGSARGGAASSICGIIDAMRRDAEI
                     YREAGIDTVIVENYGDLPFARDRVDAATTSAMTRVVDAIQREFDLTIGVNVLRNDVLT
                     AVSIAAMTGARFVRANVYVGVMQTESGIIQGCAHAVQLEIKRLGAEVEVWADLDVKHA
                     APLVERSIEEQADDAIERAFASALILTGPATGAAADPDAVARLRDHTAAPIYVGSGAT
                     PETIPAMPGATGFIVGTAMKVDGIVTNPVDPARVRAVLAAAQRS"
     gene            10242..10727
                     /locus_tag="VHR64_16775"
     CDS             10242..10727
                     /locus_tag="VHR64_16775"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX3304543.1"
                     /translation="MSDPEDTLVRSSFVRIPSEVRQASNQRYTMADLERLTGFSSRTI
                     RYYIQEGLIRAAHGRGPSATYDRDHLLRLLRIAELKTEVSALDQIRERLDDASTADLE
                     AHFAVRSGPEEERWKRVRIHPRLELNVRESADRDYRFEQAVEQIIDHARFVLERYENE
                     R"
     gene            10873..12531
                     /locus_tag="VHR64_16780"
     CDS             10873..12531
                     /locus_tag="VHR64_16780"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX3304544.1"
                     /translation="MDVDLRQLAHSALRYWWLVIALPLALGALAFIYTSRQDPLYRAE
                     VSLEVRASSTNDNAYEILLGSERQAKTYQRLVKDPAVLSAASQQLTPPIDTEALALKV
                     SSRVDSGTSLLIIGVSDTDPSTAATIANLIAQELVQVVDARNEESVRQRTGELQQRIA
                     DLKTEIDTKRAQILELERSPDAGTDAVVNQIADLKVDIERAQDNIQLFQTQIGTVIAN
                     PGDAVRVFSNAVAPNEPYAPRKLMNIVLGILLGLMLAAGLVVAIDYLDNTVKATLDFP
                     QLVGGPLLATVRTIDRIKPGRSQLFMLDDPKGVPAESIRLLRANIEFASATRELVTIS
                     LTSPNPGEGKSTISANLAVALAQAGFVTMLIDADLRRPTQHRIFGVANDRGLSTLLAY
                     PEREWDWAARETMVPNLSVIPSGPLPPNPADLLSLDRLRQILKELRDSVDVIIVDSPP
                     VLAVSDPLIIAAHVDGTALVSLGGKTRLDALRRAVQILHRGAPRIIGVVLNQQGVKGD
                     GGYYYQEYSAVSEDTRSGLRKRRSRTTATAATTATASVEQTSAD"
     gene            complement(12848..>14071)
                     /locus_tag="VHR64_16785"
     CDS             complement(12848..>14071)
                     /locus_tag="VHR64_16785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX3304545.1"
                     /translation="TVLTAAWLVEIGSFVQSPDVPEHFRIAAALHGLAAVALIVVGIF
                     GIFMWRQLRAHPAVQTVMAGLAILAMADGLWLQRWIDRNEQFGVWADVVFCIAFTTVA
                     IGSLQARLRPPRSVDTVGAGQALPLRTRQRSTSLALLTLLTLAGGQAQWGNLTKDGIE
                     IAAAAGLFVVLFVMMWEDLVAERQSVLTEEIDLLSERIDGLISQVGRDPLTGLMNRRA
                     FQERLEHELGSGRRVGHSVAIALIDVDNFKQVNDNLGHGVGDQVLQAVASVLIGACRA
                     SDIAARYAGDEFVMILPGVDEETAAQVCRRIGESVRRVNDQLRPVTSIGVTLSIGVAV
                     TYRCKRNVAQLVAIADAAMYDAKEGGKDQVVAVNADTLVSTTVWGADPATSALVETRP
                     VVEDRRIAIEHRHVG"
BASE COUNT         2394 a         4571 c         4648 g         2458 t
ORIGIN      
        1 tatcagcccg gactggactg atagcgtcga caccaccgcc atcgatccgt ccttcactaa
       61 tatcgagtcg gcggtcgcgc tggccaggtc gctcccggtc ggcgggaacc ggcggatcgt
      121 actcgtctcc gatggatccg agaatctggg gtcggttcaa aaccaggtgg cgcaagcggc
      181 gcgtgacggc gtgccgatcg atgtggtcaa agtggcgggc gccggcaacg acgacttgcg
      241 ggtcgacagc gtgaccgcgc cggcggcaat ctggcagggt gagaagccga atgtgctggt
      301 cagcgtctcg accgtcacgg cgggcaaggc ccatctcgag ctcgtggtcg ataacggggt
      361 cgtggcgacc caggatgtag atctctccgc tggtctctcg accttcgcct ttgccgtccc
      421 cgagttagga cccgggttcc acagcatgtc ggtccgcgta tcgggcgatc cgacgatgga
      481 ccagttcgcg gagaacaacg cggctccgct ggccctgatc gttcgcgacg caccgcatgt
      541 cctgctagtc gcggtggcgg gatcggatcc gagccggctg gtcaacgcgc tcaagaatcg
      601 cggcgcggaa gtcacgaatg tggcgcccga gcaggtgccg gtccagcttt cagtgcttgg
      661 cacctatgac gccttcgtgc tcgacaacgt tcccgccacg gcgctgcaag tcgagcagat
      721 cgcgggattg caggaagcga cgcgtacctt gggcaagggc ttggtcgtga tcggcggcac
      781 gtcgagctac ggccccggcc aatatgcgag cacacgcctc gaggacatgc tgccagtaac
      841 cgtcaaggtg acggacgggc gcgagcgcca gcacgtcgcg ttgctcctcg tcttcgacca
      901 cagcggctcg atgtcgtatg acccgctcca tgagaccagc aagatcgata tggccaagga
      961 ggcgatccgt ctcgccgcga atgcgctctc cgacggtgac acaatcggcg tcctcgcctt
     1021 ctccgattcg caggattggg tctttccgtt gacccagatc accggcgatt cgacgcgcca
     1081 gcaactgaac gcggccgtca gcggggtcaa ggccagcggc gggaccgaga tctacccggc
     1141 cctgcaggtc ggtctcgacg cgattcgcaa cgtcgatgcc gatgtgcgcc atgtcgtgct
     1201 gctctcggac ggcaagtcga agtccggcac gcaggatgcc tacgtgaagc tggtctccga
     1261 cgccggcgcc gaccggacga cggtctcgac gatcgcgatc ggaaacgacg ccgataccgg
     1321 cttgctgcag gcaatcgcgc aggctggcgg gggtcgctat cacttcacca accgggccga
     1381 ggagattcca caactgacgc tggaggaggc acgcagcgcc ggcgcgcaat cggtcattcg
     1441 cggggcgttc caggcggtcc agaccctgcc ctccccgatc atgaccggct tcgacgtcaa
     1501 gacgttgccg ccgcttgacg ggtacgactt caccgaggca cgtcccggcg cccaggtcat
     1561 tttggtcagc catcgcaacg atccggttct cgcgaaatgg caatacggcc tggggcgggt
     1621 ggttgcctgg acaccggacg atggagcgga tctggcgagt caatggggat cgtggcagaa
     1681 cttcgaccaa ttctggtcgt cggtattgcg ctggaccctt cccgatccgg aaaaccgggc
     1741 gatcacgacc gatgtcgcgc gggacgggcc ggacgtcgtc ctgtcgttga gcacaaccgg
     1801 tgacgcttcc ggcaatgatt atgtcgatct ctcggctatg accgcccgga tcaccggtcc
     1861 ggatggcgcc gtgactggct ccatcccgct gacccaatcc ggatcgggac agtttcagat
     1921 ccggatccgc aatgccgatt ccggggcgta caagctcgag ctgctcgatg ggagcggcac
     1981 cgcgcagggc ggggattacg gcttcacgct gcctggatcg ccagaactcc ttccctcccc
     2041 cggcggagac cggttactgg catcgatagc ggcggcgacg ggcggccgtg agctgagcat
     2101 ggacgagccc ggcgacctgt tctctgcgcc atccagctcc ggagagacat tgcagaccta
     2161 ccggccgatt tggtcgtggg cggttctcgg cgcgctcggc ctgttcctgc tcgagctgat
     2221 gctccgactc aacggttatg cccgcctgcg gtcgttgcgc ggccggctcg gatagcgccg
     2281 gctgctagaa tcagccacaa gaggcggccg cggcgaaccg gagcagactt cgcgtgatca
     2341 aggtcaaagt ccatgccgaa ggcgcggtcc ttgaccagga tcttcccctc gatcgaatca
     2401 gcgacacgct ccaatcagac aactcgctgc tctgggtcga tgtcatcgcg ccgaacgcga
     2461 aggatatcga actacttgag caggagttcg ggtttcacca tcttgcgctt gaggatgccg
     2521 cgctcgtcca cgagcggccc aagatcgatc tctacgacga ttttctgttg atcattttct
     2581 acgcgctgac gcatgagcgt agggagacac ccgggctgcg tttggagcag ctctcgctct
     2641 ttgcgggcaa gaacttcgtt gtcaccctgc accaggccga gttgccggtg ctggaccaga
     2701 ccagcgatcg gtggtgcgcg agtcacgcaa agatcaagaa cggcgcggcg gggcttctcg
     2761 tctattcgat cctggacgcg ctcgtcgatg agtacctacc gatcgccgac cggatctcgg
     2821 accgtatcga cgtgatcgag gatgccgtgt tcgggcgatt tgacgcgaac gcgcagcagg
     2881 agatcttccg gatcaagcgg aacctgctgc aaatgcgcaa ggtgctgacg cccgaacgcg
     2941 acgtgctcaa tgtgctgatg cgacgcgata cgccgatctt ctccgacgag acggtgcgct
     3001 acttccagga catctacgac cacctgctcc gggtgctgga ttcgatcgac acctaccggg
     3061 atctgctctc gagcgcgctc gacagttatc tctcggtgca atcgaatcgc atgaacaagg
     3121 tgatgaagac gctgaccgca tcgtcgatca ttttgatgac ggtcacgctg gtcgcgagca
     3181 tctacggcat gaactttgtg cacatgccgg agctgaagtg gcattacggc tatgcgctgg
     3241 ccctgggctt gatggtgacg atcgcggcca cgctcggcgc gctgttccgc aagatcgact
     3301 ggttctgacg cggggccaga ttccgggagc gatggaggtt gaagcgcggg gttaccattc
     3361 ctcgcttcgt ggatccccat gcacgattga cggagccgaa ccgtgaccgg gaatcactcc
     3421 cccaccgcag cgacggccaa cgggtcgggg cgaggcgtgg taaccccgcg ctacgtttaa
     3481 acggctactc gtcccagaga tccgggagct cctcgtcctc gtcgacttcg ttgtccggct
     3541 cactgcggac tgccacgggc tgccgatccg gcactcgcac atccacccgg gtggacatcg
     3601 gtacgacgtc ttcaggaggc acgggccgca cctgttcgga gtgcggctcg atgcggaccg
     3661 ctgtcgagcc gatttcgggc tcgggcaacg gtgcctgctc ggcgggaaga tcgcggccga
     3721 ccagctcgtt gagccactcg ccgctgagcg agatccagtc ctccagctcg ctgtagagtc
     3781 catgccccgc ctcgggatac cagaccaacg tggccagatc gccggccgcg gcagcgaggg
     3841 cgacggcctc ctcgggcggc acgaccatgt cccggccggc gccgaagacc aacagcggtg
     3901 agcgcaggcg cgggattaga tcgaccaacc cgaactcggc gaccacgtcc tcggctgtat
     3961 cgttttcgac ggccagcgtc attaactgca ggcggaccag tggattgaca tgactccacc
     4021 agtcgagtgg gtcgtatggc ggcgtgaccg cgatggcgcc gagcagccgg cggtcgtagg
     4081 ctgcgcatcg gatcgcgacc ggtccgccca ggctgaagcc ggcgatggcg accatgtccg
     4141 gctccaggtc ggggtgatcc tcgacgaagt cgaagatgcc gtcggtcatg tcgtcgcagt
     4201 cggcgatcgg cgaggtgaac gaagccgatt caccggttcc cggccagtcg atcgtcatga
     4261 cggcaatccc catatcgagc agcggaagtg cccagagcag cagttcctcc ttgcaggtcg
     4321 tcgccccatt caggagcacg agcatcggaa aggggcgctg gccggattgg ggtactgccg
     4381 cataggctgg aagggtccgc gctcgccagc gcaggctgat cttgcgcacc tcgggcatca
     4441 gtcgcggcac tgcctgggca aaggtggtca cgctggacgc gcgcaaggtg cgcagggtgc
     4501 gcgggtcggt gtcggtcacc agatgggcga tgtggaagca catggcggcg ttgatccgcg
     4561 cgactgccgc ctcctgccag cggccggagc gctcctcacc gcgagcttcc gtcaggaatc
     4621 gctgggcggc caggttccag ccgtccatcc attcacccag gccgtgaatg ccgctcagca
     4681 cctcgctgat tgtctgctcg ggaattcgca tcgcccggaa gcgatccttc atcgagcggg
     4741 gcatcccctt ggccgatcgg aaccgcaggt agcgctcgag tcggcccggc gtgacccgac
     4801 cggccaggcg ccaggcgtcg ggagcgagcc tccatcggcg aggctcgggc atcacgatct
     4861 ggcggacatg ctcattcatc ggttctggac acagctctcg gattacgggc gatcggcgcg
     4921 gccccgactc gttactccgt ccagaggcgc tcgcgggcgc gcgtcagcaa ctcctggagc
     4981 ggggcacgat cgtcctcaaa gcgggaaacc agaataccac gctcgatgcg gtggggacgc
     5041 gtgcgcagct gggcgacgaa ggtctcgatc acgacgcggg cggcatcgag acggtcatcc
     5101 cggggcgcac cggcggtggt gccgagcatc ggcagagcga tcgagcgtac tcgtagcttc
     5161 gtggccaggt cgagcgcctc gcggagcgca tgcggaatct cgagccgctt cggcggttcg
     5221 ccgagctccg tggcaatgat cacatgcagg atgtgctgga tgccacggtc ggcgagccgc
     5281 cctgagccgg tggcgaccgc gttgccgatc tcgagcggcg catgcgagcg gagctcccgc
     5341 tcgacggcct cgccggctgc gctccagagc gcgctactcg gtccagcggc aaacatggcc
     5401 cgctgattgc cggccgcgac gatggcctcg accggctgat cgacgagctc gccgacggcc
     5461 actgagatca gtgttcggcc gaaccggatg ccttcttcac ggggcaagtg ctggctagtc
     5521 atcagttgcg gctgcgcgct ccctcaatcg cagagcgcgc agtctaccat ccggccaagc
     5581 gcgggcagtg acgccaggct atatcccggg aatcttggac tcgagcaggc ggcgcaagct
     5641 cggagtgggg ctcgtctcga cataatatat gccatccgag agccactgcg gggccgcaat
     5701 tacgacgcca ttgtcgctcg tggcagcgga aatgaaggtg cctgatgtgt cgtagatctg
     5761 aagcgaatag ccgtcagaga cggcgagata tgagccgtcc ggcgaccagg tgaacccggc
     5821 gccgtttccg ttcacgtacg ggatctgcgc catcggaatg ccgggactgc tggcatcggc
     5881 cacgatcaac tgatcaccgg cagggtaggc gaccaggttt gcgccggggc tcaggaagcc
     5941 ctcgccagtt aggccgaagt cgttgggacc gaggttcgac tcgctgccgt cggcgccgac
     6001 caggagccag gaggccgtcg tggcgatcag gaatccgtca ccggtcggga tcggccggcc
     6061 gcctgacagc gcgactccag tcgaccgcca gacctccgtc gtttcgcccg aattaacgtc
     6121 gtatccatag aggatgaccg tgctgtcctg atcgccggtc tcacgcagat aataggcgga
     6181 cgtgcccgac cagccagccg ggatgtcctg taccgcgccg tccttcgacg gatctgagat
     6241 cggctcgaac ttgccggtgt ccccgtcgta gagcccgacc gacggcgtgc cgtccgcgta
     6301 ggactggtag agcagcacga ttccgagtgg cgaccagatc ggatcggcgc cggcgccgag
     6361 gtgggcgacg accgagccat cgctcatcga gacaacctgc aggtcggaac cacctgcgtc
     6421 agacgaaagc accatcaggg tgccgggcga gttgacccgg agcgggccga gcggcgcgcc
     6481 accactgctg ataccggcga tacgagcggt gtcatcgagt agggccgcgg tgttcgaggt
     6541 ctgatcgccg ttcggttctt cggtcggccc gccgattgcg gtggtttcgg ccgtcgcttc
     6601 agatgtgggt gtttccccgg cagctcccgt gccatcgacc ggcacgatct gcgtcgaatc
     6661 ggtcggttcc tcgaccggcg tctcagtcgg cacttcggtc gacggtggtg gcgcctcggt
     6721 cggcgccgtg ttgtcggatg gcccgattgt cggctgctga gccggttcct cggtcgctgt
     6781 cggctcggcc gtaggcgtcc gggttggtcg cttcggcttc ggagtctcgg tcggctcggg
     6841 agtcgcggtc tccgtcggct cccgggtttc ggtcgcggtc ggttcttccg taggcgtcct
     6901 ggtggcggtc ggttccttgg tcggcgtgcg cgttgccgtc ggctcggccg tcgccgtatt
     6961 ggtggcggtc ggctccgctg tcgccgtctt cgtcacggtc ggctcggtcg tcgccgtctc
     7021 ggttgccgtg ggcacctcgg tgggcaccgt ggtctccgtt ggggcaggcg tttcggtcgg
     7081 ttccggcttc ggcgtccgtg tcgccgtcgg ctccggcgta tcagtcggca cgctcgtttc
     7141 agtcggcgtg tcggtcggca cgctcgtctc ggtcggaacc gtggtgggcg tatcggtttc
     7201 ggtcgcggtc gatgtcggcg cctcggcagc caatacggcc gtggtcggcg tctccgtcgg
     7261 cgtctcgtcc gacgcgcgga cattgggcac gttgaccggc gtttcggttg gcatttcgac
     7321 gcccggcgtg attgcgctgc tgctcgtctg cgtgacctgg ctggcgtcgc tggtctgggt
     7381 agcgttctgg gcgacaaccg tgccggcggt gatcgtcggg ccaaggcgac cgttgtcatc
     7441 gttgccctgg aagagcgcgt agccggcgat gacgagcgcg agcatggctg cggtcgccgc
     7501 catcggggcg aaaccgaccc ggccgcccat cagaccgccg atccgctccc agatcgactg
     7561 cggttgcgcg atccgctccc ggacctgccg cgagacgatc gggctggcgg gcaggcgggg
     7621 cagggagcgg atgccggccg acatcgcgta catctgatcg gcgaattgcc ggcaggaggc
     7681 gcacgtcgcc aggtgcgaag cgaggcgctg ttcctgctgc ggggtcaggg gagcatcgag
     7741 ccgggcggaa atgagcgctt ccgcctcgga atgactaagc gggtgcgcgg ccgaaccgtg
     7801 accgcgcgag tgaccgatca ccgcgcaccc tccgagttgt cgattctggc agtttccatg
     7861 tccgccgcaa gctcctcctg gaccggatcg gtatagacat ttgcgaatgc cttccgcgcc
     7921 cgaaagaggc gaagtttcgc ggcgttttcc gtgatgtcga gcgccgccgc cgtttcagcc
     7981 agcgacagac cctcgtagtg ccggaggatc aggacggcgg cgtaatgctc cggcaatttg
     8041 cgcagcgcct gctcgacatc gtgcttgcgg ccgaccgagc gttcaatcga ttcgttcgac
     8101 gcatagtcct gtgttcccag gaatgggacc cagcggatca gcttgcgccg gcggagatgg
     8161 gagatggcgg tgttggtggc gattcggtag agccaggcct tgaaggcgag gtcgggccgg
     8221 gtattcggca gcgcccggta cgccttaacg aaagcgtcct gggtcagatc ctcggccagg
     8281 gcgcgatcgc cgaccatgcg atgaaggtag ttgaggaccg gcgcgtggta ccgctcgaag
     8341 agccgctcaa atgcgaccgg gtcacccgag cgagctcggg caaccaattc aacctcatcg
     8401 gtcacgggat aactccgcgc cggtgctggc aacgccggag atgcggctcc cagcagaaat
     8461 ctggtgcttt gtatcggtgg tgctaacgaa tcggccatcg acgaatcggt ccctctcctt
     8521 cgcaggccaa aacgcgccac cggagagacc ggtttcgctg gctgaaaact gcgcatggaa
     8581 aggcgggatt tggtcccgtg acggaatgcc catggggaag gcaggatacc aaatgccgag
     8641 ccatctctgc ctgaaaatga gcagtccatg tttcacatga aacataatct tgagcaggac
     8701 gtggggctga agctacctga cccagggaag gacccttacg tggcggacgc ggacgcacca
     8761 gcgggaagga gcaactatgg cgacaccttc gcggaggcgc tcgagcgatt cttgaattac
     8821 ctggccgact tcgggctttc ccccgaccac tgcgtcgaga gccgcatggc cagattccag
     8881 agcgaagcga ccactctgcc ggagttggct cggaacgcat tcgaggccgt cgtggcactc
     8941 gcggccgagt tcgagctcac ggttgtccga gtgagtgtcg atgggcaccg accgatcgag
     9001 ggcggacatc ggtgttgggg tacggtcgag ctcgctcggt acgctgagtc gtctggtccg
     9061 gtccgaatcg tggacggacc ggtggtcagc cgggatccgg atgacggcca atggcggatc
     9121 gacgtctggt tcgttgacga ctgatctgtg actccaagtc cacccgggtg gaccactgag
     9181 cggtgaccgc attcccgcct acaatccgcg cagtgaactt ctgatactga gacagggatg
     9241 catgaacggt caggcgttag tctatttcgg catcagcgga gcaacgctcc tgctggcgtt
     9301 gtccctcctg cgcaaacggt acgtcgagca ggatcactcg gcggtcacct cccgcggcgg
     9361 ctatacaatg tttgcgatcc tgatgctgat attttcgatc ggcgccttct tcgccggcta
     9421 cttctcacgg agctgatgcc gcccatgcct gaaccgcgcg attggccccg gatcgtcggc
     9481 gttctccacc tgccgccctt gcccggttcg gcacgcggcg gcgcggcctc ctcgatctgc
     9541 gggatcattg acgcaatgcg gcgcgacgcc gagatctacc gggaggccgg catcgacacg
     9601 gtcatcgtcg agaactacgg cgacctcccc ttcgcacgag atcgggtcga tgccgcgacg
     9661 acgagcgcga tgacccgggt cgtcgacgct atccagcgcg agttcgacct gaccatcggc
     9721 gtcaacgtgc tccgcaacga tgtcctgacc gctgtctcga tcgcggccat gaccggcgcc
     9781 cgcttcgtcc gcgccaacgt ctacgtcggg gtcatgcaga ccgagtcggg catcatccag
     9841 ggttgcgcgc atgcggtcca gctggagatc aagcgactgg gtgccgaggt cgaggtctgg
     9901 gctgatctcg atgtcaagca tgccgcgccg ctggtcgagc gctcgatcga ggagcaggcc
     9961 gacgacgcga tcgagcgggc attcgcctcg gcgttgatcc tgaccggccc agccaccggg
    10021 gctgcggccg acccggatgc ggtcgcacgg ctgcgagatc acaccgccgc gccgatctac
    10081 gttggcagcg gcgccacccc cgagacaatc ccggcgatgc ccggcgcgac cggattcatc
    10141 gtcggcacgg cgatgaaggt cgacggcatc gtgaccaacc cggtcgaccc ggctcgcgtt
    10201 cgcgcggtgc tggccgccgc ccaacggtca tgagcgccgc gatgagtgat cccgaggata
    10261 cgctggtccg ttcgagtttc gtgcgcatcc cctccgaagt gcggcaggcc tccaaccagc
    10321 gctacacgat ggcggacctg gagcggctga ccggattctc gagccggacg atccgctact
    10381 acatccagga gggtctgatc cgcgccgcgc acggccgagg cccctcggcg acgtacgacc
    10441 gcgatcatct gctgcgcctg ctgcggatcg cggagctcaa gacagaggtc agcgcgctcg
    10501 atcaaatccg ggagcggctg gatgacgcct cgaccgccga tctcgaggct cacttcgcgg
    10561 tccggagcgg tccggaagag gagcgctgga agcgagtccg aatccacccc cgcctggagc
    10621 tcaacgtgcg cgagtcggcc gaccgcgact accggttcga gcaggcagtc gagcaaatca
    10681 tcgaccacgc ccgctttgtc cttgagcgtt acgagaacga gcgctgacgc agtgtcccga
    10741 ccaatccggg actttcgggc gtcaccaccc cgcacatttg gcttagccca tccctagact
    10801 ggattttgcg gagatcggca ttgcgccgtg ccgtgcggat gcctgaacaa cccatccaga
    10861 cggggaccag atttggacgt cgacctccga caactcgccc acagtgcgct ccggtactgg
    10921 tggctcgtca tcgcattgcc cctcgccctt ggtgccctgg ccttcatcta cacctcgcgg
    10981 caggacccgc tgtaccgggc cgaagtctcg ctcgaagtgc gggcctcatc gaccaacgac
    11041 aacgcctacg agatcctgct cggctccgag cggcaggcca agacctacca gcggctggtc
    11101 aaggatccgg cggtgctgag tgccgcctcc cagcagctca ctccaccgat cgacaccgag
    11161 gcactcgcgc tgaaggtcag cagccgggtg gattccggca cgtcgctcct gatcatcggc
    11221 gtctccgaca ctgatccgtc taccgcggcc accatcgcga acctgatcgc acaggagctt
    11281 gtccaggttg tcgatgcccg caacgaggag agcgtccgcc agcgcaccgg tgagctccag
    11341 cagagaatcg ccgacctcaa gacagagatc gataccaaac gggcccagat cctcgagctc
    11401 gagcgctccc cggatgccgg gaccgacgcg gtcgtgaacc agatcgccga tctcaaggtc
    11461 gatatcgagc gagcccagga caacatccag ctcttccaga cgcagatcgg cacggtgatc
    11521 gccaatccgg gcgatgcggt gcgcgtcttc agcaacgccg tggcgccaaa tgagccctat
    11581 gccccgcgga agctgatgaa catcgtgctc ggcatcctgc tcgggctgat gctggcagcc
    11641 ggcctggtcg tggccatcga ttacctcgac aacacggtca aggccacgct cgacttcccg
    11701 caactggtcg gcggtccgct gctggcgacc gtccgcacga tcgatcggat caagcccggc
    11761 cggtcccagc tcttcatgct cgacgacccg aagggggtgc cggcggagtc gatccggctc
    11821 ctgcgcgcca acatcgaatt cgcgtcggcc acgcgggagc tggttacgat cagcctgacc
    11881 agcccgaacc cgggcgaagg caagagcacg atctcggcca acctggcggt cgcgctggcc
    11941 caggccggct tcgtgacgat gctgatcgac gccgatctgc gccgaccgac ccagcatcgc
    12001 attttcgggg tcgcgaacga tcgcggcctg agcacgctcc tggcctaccc ggagcgcgaa
    12061 tgggactggg ccgcgcgcga gacgatggtg ccgaacctgt cggtgatccc ctccggaccg
    12121 ttaccgccaa acccggccga tctcctgagc ctcgatcgct tgcgtcagat cctgaaggag
    12181 ctgcgcgatt cggtcgacgt gatcatcgtc gactcgccgc cggtcctcgc cgtcagcgat
    12241 ccgctgatca tcgccgccca tgtcgatggc accgcgctgg tcagtctcgg cgggaagacc
    12301 cggctggatg cgctcaggcg ggccgtccag atcctgcacc ggggtgcgcc gcggatcatc
    12361 ggcgtcgtgc tcaaccagca gggcgtgaag ggcgatggcg ggtactacta ccaggagtac
    12421 agcgccgtct cggaggacac ccgcagcggg ctccgcaagc gccgatcacg aacgactgca
    12481 actgccgcga cgaccgcgac tgcctcagtc gaacagacct cggcggatta ggttcaacca
    12541 acgctggtcg tgtggcgggt agccgccgta ccacgcttcc gttcggatga gccggttccc
    12601 cactgtggcc acatcctgcg ccgcccgaag ctaaaggttc gggctgagta acgggaagcc
    12661 cactgaaggg gctaccaagg aaatcggcat cggttcgccg atctattagt ccctctagcg
    12721 gacttctcct ggttcagtcc gggcatttat ccccgggcgg cgccggacgg ccgatgccct
    12781 gttcgaagcg agctaatcca ctggatgtga ggtatggccc tgcggcgcgc tccgtagttg
    12841 agcagcgtca gccgacgtgg cggtgctcta tggcgatgcg ccggtcctcg accaccggcc
    12901 gggtctcgac cagcgccgag gtcgcgggat cggcgcccca gaccgtggtg gagaccagtg
    12961 tgtcggcatt gacggcgacg acctggtcct tgccgccttc cttggcgtcg tacatcgcgg
    13021 catcggcgat cgcgacgagc tgcgccacgt tccgcttgca gcgataggtg acggcgacgc
    13081 cgatcgagag cgtgaccccg atgctcgtga ctggtcggag ttgatcattg accctgcgca
    13141 cgctctcgcc gatcctccgg cacacctgtg cggccgtctc ctcatcgacg cccggcagga
    13201 tcatcacgaa ctcgtcgccg gcatagcgtg cagcaatgtc gctagcccgg caggctccga
    13261 tcaggaccga cgcgaccgcc tgcaggacct gatcgccgac gccgtggccg aggttgtcat
    13321 tgacctgctt gaagttgtcg acgtcgatca gcgcgatcgc aaccgaatga cccactcgcc
    13381 ggccggagcc gagctcatgc tcgagccgct cctggaacgc gcgccggttc atcaggccgg
    13441 tcagcgggtc ccggccgacc tgggagataa ggccatcgat ccgctcgctg agcaggtcga
    13501 tctcttcggt caggacagat tggcgctcgg caaccaggtc ctcccacatc atcacgaaga
    13561 ggaccacgaa gagccccgcc gctgcggcaa tctcaatgcc gtccttcgtc aggttgcccc
    13621 attgtgcctg cccgccggcg agcgtcagca aagtgagcag agcgagcgag gtcgaccgct
    13681 gcctggtccg gaggggaagc gcctgaccgg caccaacggt atcgacgctc ctcggcgggc
    13741 gcaaccgggc ctgcaagctg ccgatcgcga cggtggtgaa ggcgatgcag aagacgacgt
    13801 cggcccagac gccgaactgc tcgttccggt cgatccagcg ctgcagccag aggccgtcgg
    13861 ccatggccag aatcgccaga ccggccatca ccgtctgcac ggccgggtgc gcacgcagct
    13921 gccgccacat gaagatgcca aagatgccga ccacgatcag ggcgaccgcc gccaacccat
    13981 gcagggccgc ggcaatccgg aaatgctccg ggacgtccgg cgactgcacg aaggatccga
    14041 tctcgaccag ccaggcggcc gtcaggacgg t
//
LOCUS       DATAOZ010000667         3765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2890178, whole genome shotgun sequence.
ACCESSION   DATAOZ010000667 DATAOZ010000000
VERSION     DATAOZ010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2890178"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VHR64_16790"
     CDS             <1..343
                     /locus_tag="VHR64_16790"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX3304546.1"
                     /translation="KYVSHIPGMPDMDREGDKQLVGSMHAAFPDLRFEVGAQIAEGDR
                     VLHTLIGRGTHRAEFMNIPATGKTIEVTGMNINRIANGKIAESWGVIDVLGLLQQLGV
                     VPTPGGEPSAV"
     gene            492..1046
                     /locus_tag="VHR64_16795"
     CDS             492..1046
                     /locus_tag="VHR64_16795"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEX3304547.1"
                     /translation="MDSEPIGEDARTLLALANAERIRMLALIADQELTSAGIAAALGL
                     SSEAVTAHLSILGQAGLIATSKDRHERRVQVRMERLEEIEAACREQLRVGHEADDGPS
                     AIPPTISQFFQHGRLTTFPSKHSRYLEVLGVLITDFAPDSDYPEAEVNQILLRRNEDF
                     ATLRRDLVDLGFMTRANGIYRRIR"
     gene            1125..2489
                     /locus_tag="VHR64_16800"
     CDS             1125..2489
                     /locus_tag="VHR64_16800"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX3304548.1"
                     /translation="MGLDLVFGHIEQHRDAFIERLCAYLRMPSISAQGVGIAENADFL
                     VSFLSGMGLEARQIGTAGWPLVYARRDDVPGGPRILLYGHYDVQPPDPLDQWISPPFL
                     PEIRDGRIYARGAGDNKGQHFANLLAIESLLAVNGALPCNVIVLLEGEEEVGSPNIAE
                     FVRTHPDLIDLDLVVTSDGPVHDSGRQTIEFGVRGVADFELRARGANRDLHSGNFGGV
                     APNPIWTLVHALASMKNPAGEITIEGFYDSIVPPTELEWAAIAAMPDGTALVMADLGL
                     TRLDAPLDRPFYDRLMAWPTLTINGFHGGYGGPGSKTVLPSEAFVKCDVRLVPNQTVA
                     EIHEKIAAHLRTHASEVEFIPGGGMDPSKTPLDSPFTEPIRRAIEAATGEDPLLVPAM
                     GGSLPEYVWTKILGKPAFVVPYANHDERNHAPNENIEVARFIKGIKIGAALLTEVGSG
                     RLGT"
     gene            complement(2566..>3765)
                     /locus_tag="VHR64_16805"
     CDS             complement(2566..>3765)
                     /locus_tag="VHR64_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX3304549.1"
                     /translation="RNALAGRAGFDKKDFTSKETYEVMDLCLSCKACKTECPSSVDMA
                     KLKMEFLAHYHDAHGTPLRARLFGHIHTISRLTRPVAPIANFVLRTKLGRPMLSAMGV
                     HPDRRLSPFQFKTFSSWWRKHDANLPLDRKTRGKVVYFHDTFTEYNYPRIGIAAVQLL
                     EAAGFEVVIEHRHTCCGRPALSKGLLNDAKRAAARNVHALADYAKAGIPIVGTEPSCI
                     LTLRDEYVDLLPGDEDVEAVASHSYMIDEFLARLESTGRLGVGWKSDPQPVMFHGHCH
                     QKALIGMGPSMAILKAAGCAATESGAGCCGMAGSFGYEAEHYDVSRKIGEERLFPAVA
                     EQGEDTVIAVSGVSCRQQIEHFTERKTTHIVEVLAGRVARPWEPPAVADLPVEELLEE
                     TIEVAAD"
BASE COUNT          645 a         1228 c         1243 g          649 t
ORIGIN      
        1 caagtacgtc agccacattc ccggcatgcc ggatatggat cgcgagggcg ataagcagct
       61 ggtcggatcg atgcacgccg ccttcccgga cctgcgcttc gaggtcggcg cgcagattgc
      121 cgagggcgat cgcgtcctcc acaccctgat cggccgcggc acgcataggg cggagttcat
      181 gaacatcccg gccaccggca agacgatcga ggttacgggc atgaacatca accggatcgc
      241 aaacggcaag atcgccgaat cgtggggcgt gatcgacgtc ctcggcctgc tccaacagct
      301 cggcgtagtg cccacacccg gcggggagcc aagcgccgtc tagcgatgct cccgtcgtgg
      361 cgtagcgcgg cccgcccctg ggccgcttcg gtctcgggtg tgccggtcga tgaggtcggt
      421 tggctccgtc aatcgcgcgt gcagtcgacc cagccggcca cggggggccg gcgctacgct
      481 aaagttctgt catggactcc gaaccgatag gtgaagatgc gcggacgttg ctggcgctgg
      541 cgaatgccga gcgaatccgc atgctggcgc tgatcgccga ccaggagttg acctcggccg
      601 gcatcgcagc tgctctgggc ttgtcatccg aggccgtgac tgcgcatctc agcatcctcg
      661 ggcaggcggg cctgatcgca accagcaagg accggcacga gcggcgggtc caggtgcgta
      721 tggagcggct cgaagagatc gaggcggcgt gccgggagca gctccgggtc gggcacgagg
      781 cggatgacgg gccgagtgcc attccgccga ccatcagcca gttcttccag catggccgcc
      841 tgaccacatt tccgtcgaag cactcccggt atctcgaagt gctgggagtt ctgatcaccg
      901 acttcgcgcc ggattcagac tacccggagg cggaggtcaa ccagatcctg ctccgccgca
      961 acgaggactt cgccaccctc cgccgcgacc tcgtcgacct gggcttcatg acgcgcgcca
     1021 acggcatcta ccgccggatt cgataaccca cgccaaccca attgcaatgc ccccgtacaa
     1081 ttctgggttg gatctcgggt ggaacactgg cgggtgcagt tggcatgggt ttggacctcg
     1141 tcttcggtca catcgagcag catcgcgatg cgttcatcga gcggttgtgc gcctatctgc
     1201 ggatgccgtc gatctcggcg cagggagttg ggattgccga gaacgcagat ttcctggtct
     1261 cgttcctctc cgggatgggg ttagaggcgc gacagatcgg gacggctggg tggccgctgg
     1321 tctatgcccg gcgagatgat gtcccgggcg gtccgcgcat cctgctctac ggtcattacg
     1381 acgtgcagcc gccggacccg ctcgatcagt ggatctcgcc gccattcctg cccgagatcc
     1441 gggacggccg gatctatgca cgcggcgccg gcgacaacaa gggacagcat ttcgccaacc
     1501 tgctggcgat cgagtcgctg ctggcggtca acggtgcgct cccctgcaac gtgatcgtcc
     1561 tgctcgaggg cgaggaggag gtcggctcgc cgaatatcgc cgagttcgtg aggacgcacc
     1621 ccgatctgat cgatctcgac ctggtcgtga ccagcgacgg gccggtccac gacagcggcc
     1681 gccagacaat cgagttcggc gtgcggggcg tggccgattt cgagctgcga gccaggggag
     1741 ccaatcgcga tttgcactcc ggcaatttcg gcggggtcgc gccaaacccg atctggacgc
     1801 tggtccatgc gctagcgtcc atgaagaacc cggccggcga gatcacgatc gaaggcttct
     1861 acgactcgat cgtgccgccg accgagctcg aatgggccgc gatcgccgcg atgcccgacg
     1921 gcaccgcgct ggtgatggcg gacctcgggc taacccgcct cgatgcgccg ctcgaccggc
     1981 cgttctacga ccggctaatg gcgtggccga cgctgacgat caacggcttc cacggcgggt
     2041 acggcgggcc gggctccaag acggtgctgc catccgaggc gttcgtgaaa tgcgacgtcc
     2101 ggctggttcc gaaccagact gtggccgaga tccacgagaa aatcgccgcg cacctgcgga
     2161 cccatgcatc ggaggtcgag ttcattcctg gcggcgggat ggacccgtcg aagacgccgc
     2221 tcgactcccc cttcaccgaa ccgatccgcc gcgcgatcga ggccgcgacc ggcgaggatc
     2281 ccctgctggt cccagccatg ggcgggagcc tgcccgagta cgtctggacc aagatcctgg
     2341 gcaagccggc cttcgtcgtc ccctacgcca accacgacga gcgcaaccac gcccccaacg
     2401 agaacatcga ggtcgcccga ttcatcaagg gcatcaagat cggtgccgcc ctgctgacgg
     2461 aagttggtag cggacgactt ggaacgtagc gcggggttac cacgccccgc ccggacccga
     2521 tgacagccac ttcgctggag gaaccaagcc cgaaacccgt catgctcagt ccgcggcgac
     2581 ttcgatcgtc tcttcgagca gctcctcgac gggaagatcg gcgaccgccg ggggttccca
     2641 ggggcgggcg actctccctg cgaggacttc gacgatgtgg gttgtcttgc gctcggtgaa
     2701 gtgctcgatt tgctggcggc aggagacgcc tgagacggcg atcaccgtat cttcaccctg
     2761 ctccgccacc gccgggaaga ggcgctcctc gccgatcttg cgcgagacgt cgtagtgctc
     2821 ggcctcgtac ccgaacgatc cggccatgcc gcagcagccg gcgccgctct cggtcgccgc
     2881 acagccggct gccttcagga tcgccatcga cggccccatc ccgatcaacg ccttctggtg
     2941 gcaatggccg tggaacatga ccggctgcgg atcgctcttc cagccgaccc cgagccggcc
     3001 ggtcgattcc agccgggcca ggaattcgtc gatcatgtag gagtggctgg cgaccgcctc
     3061 gacatcctca tcgccgggca ggagatcgac gtactcgtcg cgcagggtca ggatgcagct
     3121 cggctcggtt ccgacgatcg ggatgccggc cttggcgtag tccgcgagcg cgtgaacgtt
     3181 gcgtgccgcc gcccgtttgg catcgttcag caaaccttta gatagcgccg gtcggccgca
     3241 gcaggtgtgg cggtgctcga tcacgacctc gaatccggct gcttcgagca actggacggc
     3301 ggcgatgccg atccgcgggt agttgtattc ggtgaaggtg tcgtggaaat agacgacctt
     3361 gccgcgagtc ttccgatcca gcggcaggtt agcgtcatgc tttctccacc agctcgaaaa
     3421 agtcttgaac tggaacggcg agagcctgcg atccgggtgg acgcccatcg cgctcaacat
     3481 tggccggccg agcttcgtgc gcaagacgaa gttggcgatc ggtgcgaccg gccgggtcag
     3541 ccgggagatc gtatggatgt ggccgaagag ccgggcccgc agcggcgtgc cgtgcgcgtc
     3601 gtggtaatgg gcgaggaact ccatcttgag cttggccatg tcgacgctcg aaggacactc
     3661 cgtcttgcag gccttgcagg agaggcagag atccatcacc tcgtaggttt ccttcgaggt
     3721 gaagtctttc ttgtcgaagc cggcccggcc cgccagcgcg ttgcg
//
LOCUS       DATAOZ010000668         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6308051, whole genome shotgun sequence.
ACCESSION   DATAOZ010000668 DATAOZ010000000
VERSION     DATAOZ010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6308051"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1007)
                     /locus_tag="VHR64_16810"
     CDS             complement(108..1007)
                     /locus_tag="VHR64_16810"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3304550.1"
                     /translation="MEKSKRSGANLRRRLIPYGLLAPGSIWLILFFVVPMYFMGEMSL
                     RSGVPNTPEGFTFSWQFSNYSDALAGRGEQIVRTFFYAGSATIIALLIAYPLAYAIAL
                     KVNPRWRLPLLFAVIAPFFTTYLIRTVAWETILADDSPIVNIMKSLQIIPEDGRLLAT
                     SGAVIAGLTYNFLPFMILPIYASLERVDTSLIEAGKDLYASARQAFLRVTLPLTTPGV
                     IAGVLLTFIPASGDYINAEFLGGANNTMIGNKIQSTYLEQSDYPKAAALSFLMMAVVL
                     TIVFIYIRLAGTEAFTGSEEEAR"
     gene            complement(1037..2185)
                     /locus_tag="VHR64_16815"
     CDS             complement(1037..2185)
                     /locus_tag="VHR64_16815"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX3304551.1"
                     /translation="MRSKTRFRALIAAGGALAAAVVLAACGGDGDNVGGGNAGEVTVA
                     KGGPASGSVLISNWPGYVDPKSTIADFDEQTGLNTTYKEDVNDNVVFFNKLKPQLDQG
                     SSGGRSLFVVTDWMAKRMYDLGYLQQINHADLPTVFQNILPQFEKSTTDPERKYSIPW
                     QGGQTGIWVDTAKAPEIDSVSDLFDPKYKGKVTMLTEMTDTVPLILQSEGINPDEATK
                     DDWLAAIDKLSQARDSGQIRKFTGNDYTEDLTSGNIVAALGWSGDGSLIGNENAEWRR
                     PTDGCDLFFDQAVIPVGAPNTPAALAFLNFAYTPANAAKITAYVLYVTPVNGVRPILQ
                     KEDPKVANDPKIFPTEAEIAPCSEDPLPPGSPEDIAEVEAAFQEVVSG"
BASE COUNT          376 a          711 c          726 g          442 t
ORIGIN      
        1 cccgtgtaga tcttgaaccc gttccgcctc acccacgacc aggcagagcg tggagccgcc
       61 gagagcgctg gtctcgctgg cgcggttgcg gtcgctgatt ccatgcccta ccgcgcctcc
      121 tcctcgctgc cggtgaaggc ctcggtccct gcgagccgga tgtagatgaa gacgatcgtc
      181 agcaccaccg ccatcatcag gaaggagagc gcggcggcct ttggatagtc gctctgctcc
      241 aggtaggtgg actggatctt gttgccgatc atggtgttgt tggcgccgcc gaggaattcc
      301 gcgttgatgt agtcgccgga ggccgggatg aaggtcagca gcacgcccgc gatgacgccc
      361 ggtgtcgtca gcggcaaagt gaccctgagg aaggcctgcc tcgcggatgc gtagagatcc
      421 ttgcccgctt cgatcagact ggtgtcgacc cgctcgagac tggcatagat cggcaggatc
      481 atgaagggca ggaagttgta ggtgagtcct gcgatgaccg ccccggaggt ggccagcagg
      541 cgaccgtctt ccgggatgat ctggagcgac ttcatgatgt tcacgatcgg gctgtcgtcg
      601 gcgagaatcg tttcccaggc gacggtcctg atcaggtagg tggtgaagaa gggtgcaatg
      661 actgcgaata gcaacggaag acgccaacgc ggattgacct tgagcgcgat tgcgtacgcc
      721 agcgggtagg cgatcaacag agcgatgatc gtcgccgatc ccgcgtagaa gaaggtgcgc
      781 acgatttgct ccccgcggcc agcaagtgca tccgagtagt tggagaactg ccagctgaac
      841 gtgaatccct caggggtgtt cggcaccccg gagcgcagcg acatctcgcc catgaagtac
      901 atggggacca cgaagaacag gatcagccag atcgagccgg gcgcgagcag cccgtagggg
      961 atcaggcgcc ggcggaggtt cgccccagag cgtttgctct tctccatctc cttgtcctcc
     1021 gccggcgccg tatccctcag ccggaaacca cctcctggaa ggcagcctcg acctcggcga
     1081 tgtcctccgg actacccggc gggagtgggt cctcggagca tggcgcgatc tccgcttccg
     1141 tcgggaagat cttcgggtca ttggccacct tcgggtcttc cttttgcagg atcggccgca
     1201 cgccgttgac cggcgtcacg tataggacgt aagccgtgat cttcgccgcg ttcgcgggtg
     1261 tgtaggcgaa attcaagaac gcgagcgccg cgggcgtgtt cggcgcaccg accggaatca
     1321 cggcctgatc gaagaacagg tcgcatccgt ccgtcggcct gcgccactcg gcgttctcat
     1381 tcccgatcag ggagccgtct ccggaccagc ccagcgcggc gacgatgttg ccgctggtca
     1441 gatcctccgt gtaatcgttg ccggtgaact tgcggatctg ccccgagtcg cgcgcctggc
     1501 tcagcttgtc gatcgcggcg agccagtcgt ccttcgtcgc ctcgtccggg ttgatcccct
     1561 cgctctggag gatgagcgga acggtgtccg tcatctcggt cagcatcgtc accttgccct
     1621 tgtacttggg gtcgaacagg tcggagaccg agtcgatctc gggcgccttg gccgtgtcga
     1681 cccagatgcc ggtctggcca ccctgccacg gaatcgagta cttgcgctcg ggatccgtgg
     1741 tggacttctc gaactgcggc aggatgttct ggaacaccgt cggcaggtcc gcatggttga
     1801 tctgctgcag gtagccgagg tcgtacatcc gcttcgccat ccagtcggtg acgacgaaga
     1861 ggctgcggcc gcccgagctg ccctgatcga gctgtggctt cagcttgttg aagaagacga
     1921 cgttgtcgtt gacgtcctcc ttgtacgtgg tgttgagacc ggtctgctca tcgaagtcgg
     1981 cgatggtgga cttcgggtcg acatagccgg gccagtttga gatcagaacc gagccgctcg
     2041 cgggcccgcc cttggcaacg gttacctcgc ccgcattgcc gccgccgaca ttgtcgccgt
     2101 cgcccccgca ggcggccagg acgactgcgg cagccaacgc cccgcccgcc gcgatcaacg
     2161 cccgaaatct cgtcttgctc ctcacgttcc cgccttgtct ggctcgttca agctgtttcc
     2221 gccggcctct cgctccggac cgcgccattc gtttc
//
LOCUS       DATAOZ010000669         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4732929, whole genome shotgun sequence.
ACCESSION   DATAOZ010000669 DATAOZ010000000
VERSION     DATAOZ010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4732929"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VHR64_16820"
     CDS             complement(<1..488)
                     /locus_tag="VHR64_16820"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX3304552.1"
                     /translation="MSQLQIAAAQIQHETNVFSSVRTDMAAFQRSGLKFGPEIEAERG
                     TNSAFGGFFAAAERHDIDLLPILSVWVTPSGIVSADAITELVTHLIEQLIASQPDGVA
                     LSLHGAMVSEIDRDGDGWILDQIREAVGWDIPVVAELDLHANISTRMIKAADILVGYD
                     TYP"
     gene            complement(485..1333)
                     /locus_tag="VHR64_16825"
     CDS             complement(485..1333)
                     /locus_tag="VHR64_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX3304553.1"
                     /translation="MSITPTDLSQPVIISAALTGSWPTKAENPAVPITEDEIAEAALA
                     AHNAGAAVVHLHVRNEHGKVTCDPARYAKVRDLIRAAGSDVIINMSTGGGAGQTTDEQ
                     RMEPVSLKPEIASFDCGSCNFGKTVFINSPSFLDELAQRMNDHGVLPEIECFEPGHVA
                     NAIRLIDEGKLKPPHWFQVVLNVRGGSPGTVRQLLHMVEMLPAGALWSVCAIGRAQLP
                     INLAAMAMGGHVRTGLEDNIYYHKGQLAESNAQLVARLARIAGEIGRPVATSTRAREM
                     LGLPPL"
     gene            complement(1465..1878)
                     /locus_tag="VHR64_16830"
     CDS             complement(1465..1878)
                     /locus_tag="VHR64_16830"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX3304554.1"
                     /translation="MNIRWRGLLALAVVLLVIAGGALRVSANSAGISKGTQPVSMVHA
                     VESGAHSFARLVGLEGDESQVAPGTLDDGKDLLPQAKISIEQAIAAAQSSASGDIGEI
                     DLEHYQGHLVFNVDVGDHDVKVDATTGQVLGSGTD"
     gene            complement(1980..>2209)
                     /locus_tag="VHR64_16835"
     CDS             complement(1980..>2209)
                     /locus_tag="VHR64_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015856026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX3304555.1"
                     /translation="VRIRVEDSGPGIPPDDRERIFDRFYRVDTGRDRSRGGAGLGLSI
                     CRAIVEAHGGTIAVGDSGLGGARFDVLLPIP"
BASE COUNT          379 a          727 c          702 g          401 t
ORIGIN      
        1 gggtacgtgt cgtacccgac caggatgtcc gccgccttga tcatgcgagt cgagatattg
       61 gcatggagat cgagctcggc cacgaccggg atgtcccagc cgaccgcctc ccggatctgg
      121 tcgaggatcc agccgtcgcc gtcacggtcg atctcgctaa ccatcgcgcc gtgaagggag
      181 agggcgacac cgtcaggctg cgacgcaatc agttgctcga tcagatgcgt gaccagctcg
      241 gtgatcgcat cggccgatac gatgccggac ggcgtcaccc agaccgacag gatcggcaga
      301 aggtcgatgt cgtgccgctc agccgccgcg aagaagccgc cgaaggcaga gttcgtgccg
      361 cgctcggctt cgatctccgg gccgaatttc agcccggatc gctggaacgc ggccatgtcg
      421 gtccggaccg agctgaagac attcgtctcg tgctgaatct gcgccgctgc gatctgcaat
      481 tgactcacag cggcggcagc ccgagcatct cgcgcgcccg ggtcgacgtt gcgaccggcc
      541 ggccgatctc gcctgcgatc cgagccagtc gggcgaccag ctgggcgttg ctctcggcga
      601 gctgcccctt gtggtagtag atattgtcct cgagcccggt ccgaacgtgc ccgcccatgg
      661 ccatcgccgc caggttaatc ggcaactgcg cccggccgat cgcgcacact gaccagagcg
      721 ctccagccgg cagcatctcg accatgtgca ggagctgcct gaccgtcccc gggctgccgc
      781 cgcgcacgtt cagcacgacc tggaaccagt gcggcggctt cagcttgcct tcgtcgatca
      841 ggcgaatggc attggcgacg tgaccgggct caaaacactc gatttcgggc agcacgccgt
      901 gatcgttcat gcgctgagca agctcatcga ggaaggaggg cgagttgatg aagacggtct
      961 tgccgaagtt gcaggagccg cagtcgaacg aggcgatctc cggcttgagt gagaccggct
     1021 ccatgcgctg ctcgtcggtc gtctgaccgg cgccgccacc ggtcgacata ttgatgatca
     1081 cgtccgatcc agccgctcga atcaggtcac gtacctttgc gtaccgtgct gggtcgcagg
     1141 tcactttgcc gtgctcgttg cggacgtgga ggtggacgac ggccgcgccg gcgttgtgcg
     1201 ccgcgagtgc cgcctcggcg atctcgtctt cggtgatcgg caccgcggga ttttcagctt
     1261 tggtcggcca cgagccggtc aacgcggcgg agatgatgac cggttggctg agatcggtag
     1321 gggtgatcga cacgcgacgg tcctcctgtc aggcgagatt ccggatgcgc gagtctagca
     1381 tcgcgatgcg ccgatccgga ccaggcacga aagcggcggg tccgcattag gcccgccgct
     1441 tgatttgata tcgcgagttg cttttcagtc ggtgcctgat ccgagcacct gacccgtcgt
     1501 cgcgtcgacc ttcacgtcat ggtcgccgac gtcgacgttg aacacgaggt gaccctggta
     1561 atgctcgagg tcgatctcgc cgatatcccc cgaggcgctc gactgtgccg cggcaatcgc
     1621 ttgctcgatg ctgatcttcg cctgcggcag cagatccttg ccatcatcga gcgtgcccgg
     1681 cgccacctgc gattcgtcgc cttcgaggcc gaccagcctg gcgaatgagt gcgcgcccga
     1741 ttcgacggca tgcaccatcg agacaggctg agtccccttg gatatcccgg ccgagttcgc
     1801 gctgacgcga agcgctccgc cggcaatgac cagaagcacc acggccagcg ccagcagtcc
     1861 tctccatcga atgttcatat gcaatcccct cgttccctgc aatgtccagt ccacggactc
     1921 ggcccgggcc gccttcgtgc ccggtgacaa gaatatggaa cagcatcgtt aagttgccgt
     1981 taaggaatcg gtagcagcac gtcgaagcgg gcgccgccga ggccggaatc gccgaccgcg
     2041 atcgtccctc catgcgcctc aacgattgcc cggcagatcg agaggccgag acctgccccg
     2101 cctctcgatc gatcgcgtcc cgtatccacg cggtagaagc ggtcgaagat tcgctcccgg
     2161 tcgtccggcg ggatgccggg cccggaatct tcgaccctga tgcggacac
//
LOCUS       DATAOZ010000670         5207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6309447, whole genome shotgun sequence.
ACCESSION   DATAOZ010000670 DATAOZ010000000
VERSION     DATAOZ010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6309447"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VHR64_16840"
     CDS             complement(<1..585)
                     /locus_tag="VHR64_16840"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX3304556.1"
                     /translation="MSHDVVIIGGGPAGLSAALLLGRGRRNVVLLDTGKGRNAPSDAS
                     HSFFTRDGTSPAELRRIGHEQLRPYETVAVRPDAAVAVSGTVGQFQVMLAGGDTLESR
                     AVILATGVRDILPNIPGMDRYWGKGVFQCPFCDGWEHRDQPMALLAPPEMVLESVHLY
                     RTWSKNLTVLTNGEWEPDRELSAQLADLGVDVIAD"
     gene            709..2394
                     /locus_tag="VHR64_16845"
     CDS             709..2394
                     /locus_tag="VHR64_16845"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX3304557.1"
                     /translation="MAESFDFVIVGGGSAGCVLANRLSARSGQTVALVEAGADTPPDH
                     DDQVLWDSYPIIAYFDRRHQWTNLRVRTRPAGPLRTYEQARVMGGGSSINGQMANRGQ
                     PFDYDEWAEEGAAGWSWQSVAPYFRKLERDLDFSGDHHGAEGPVPIRRVRKTDWPAFS
                     AAAAEAVTDSGLPWIEDQNTSEFLPACFPITINNEQDRRVSSAVAYLSRDVRQRPNLT
                     IMAQTRAIRLLMDGTRCTGVEVEGPDGRREMRAGQVILSAGAIHSPAMLMRAGIGPGE
                     HLTQRGIEVAADRAGVGQNLMDHPMISVSAFMKRSARLAKAQRRHIFLGLRWSSGLAD
                     CPPADMYAVAYNRGAWHPVGWRIGGFLTWINKSYSRGWVKLADASPEREPEVQFDLLG
                     DERDLGRMRDAVRRMIGYFDHPALKAVAGEPFPTSYTERYKRLAAVNRRNLAITTGLG
                     WTLDGPAQLRERMLRGVVTDGTSYDAILDDDTALGDYLRATATGIWHASGTCRMGDPR
                     DPQAVTDGNGRVIGIEGLRVCDASIMPTIPCANPNVPIMMMAERISDLILNEA"
     gene            2407..3477
                     /locus_tag="VHR64_16850"
     CDS             2407..3477
                     /locus_tag="VHR64_16850"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX3304558.1"
                     /translation="MRFSRMITAVDAHAGGEPGRVITGGVGPIPGDSVFAQMQFLAEE
                     QDEIRKLMLREPRGYPGLCCNLLVPPTNPMADMGFIIMEQMEYPAMSGSNTICVTTVL
                     LETGILPMTEPVTELTLESPAGLVKVRADCANGKVTRVTFENVPSFALHLNVPVEVPN
                     IGTIKVDVAWGGMMYVLFEGWQVGLHLTPDEGADCVKYGEMVKAATREQYPSVHPEIP
                     ELTGPTIAMITGNPMSEHAHGRNTVVISTGDFDWENPSTWTGALDRSACGTGTSARMA
                     VLNAKGKLGLNRDYVHESILGTTFIGRLIRKTKVGSYDAVVPTISGQAWITGIAQYLL
                     DPTDPFPEGYRVADIWGGISAV"
     gene            3570..4178
                     /locus_tag="VHR64_16855"
     CDS             3570..4178
                     /locus_tag="VHR64_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304559.1"
                     /translation="MQVPAAQPQIDDRVLREIHHHLRAEERLLWLGQPAKDTPMNAAQ
                     KVNVIGGSVALAGFIVIGLLTSYWGFFAEVAILICFGAGIAAGLIVFTVSNKKVAERY
                     STYVLTDQRAFSFIYDGELAVVSSLPLSTHTKISSRIKTATTGSLLFTRAHRKAPDLP
                     RLTKLLPVNKRSSRLTFHGVHQPEYVQQVALWAAAHHNDLGA"
     gene            complement(4195..4647)
                     /locus_tag="VHR64_16860"
     CDS             complement(4195..4647)
                     /locus_tag="VHR64_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304560.1"
                     /translation="MTKQEDTVRVRRLDDGSWVEELPDGTCVPMRTGGTDWARLRAMT
                     EEDIEYNALMDPDFEPFLTDEDREKIKKLPDPKRIRRKLKLTQKEFSRRYRIPLGTLR
                     DWERRARFPDSAARAYLTVISQEPERIAAILAAPDPTNEPARELAKAS"
BASE COUNT          984 a         1609 c         1633 g          981 t
ORIGIN      
        1 atcggcgatc acatcgacgc ccagatctgc gagctgtgca ctcagctcac ggtccggctc
       61 ccactcgccg ttggtcagga cagtcaggtt cttgctccac gtgcggtaca ggtggacaga
      121 ttcgaggacc atctccggcg gcgccagcag ggccatcggt tggtcgcggt gctcccaccc
      181 atcgcagaac ggacactgga agacgccctt cccccagtag cgatccatgc ctggtatgtt
      241 cggcaagatg tcccggacac cggtggcgag gatcacggcg cgtgattcca acgtgtcgcc
      301 gccggccagc atcacctgga actgcccaac cgtgcccgac accgcaactg ccgcatcagg
      361 ccgtacggca acggtttcat acggccgcaa ctgctcatgg ccgatgcggc gaagctcggc
      421 cggcgatgtg ccgtcccgcg tgaagaagct gtgcgatgca tcggagggcg cgtttcgacc
      481 cttcccggtg tcgagcaaca ccacgttgcg tcgtccgcga ccgagcagaa gcgccgcact
      541 cagccccgcc ggtcctcctc caatgattac gacgtcatgg ctcactgcct acctcccatt
      601 attgatacta cgtcgcatta ctctacagca ggacgagccc cgacgccaga tcattcccgg
      661 ggcatctaca atcccggcag ttcagtggac gaggatgggc tggtattggt ggcggaatca
      721 tttgactttg tgatcgttgg cggaggcagc gcgggctgtg tactcgccaa ccggctttcg
      781 gcgcgctccg ggcagacggt ggcgctggtt gaggccggcg ccgatacccc acccgatcac
      841 gacgaccagg tgctctggga cagctacccg atcatcgcct acttcgaccg tcgccatcag
      901 tggaccaacc tccgggttcg cacccggccg gccggaccgc ttcgcaccta cgagcaggcg
      961 cgggtcatgg gcggcggatc gagcatcaat gggcagatgg cgaaccgcgg ccagccattc
     1021 gattatgacg agtgggccga ggaaggtgcg gctggctgga gctggcagtc ggtcgcgccg
     1081 tatttccgca agctcgagcg ggacctggac ttctccggcg accaccacgg cgccgaaggt
     1141 ccggttccga ttcgccgcgt gcgcaagacc gactggccgg ccttttcggc tgcggccgcc
     1201 gaggcagtaa ccgattctgg cctgccgtgg atcgaggatc agaacacctc cgagttcctg
     1261 ccggcctgct tcccaatcac gatcaacaac gagcaggatc gccgggtctc gtcagcggtc
     1321 gcgtatctga gccgggatgt tcgccaacgc ccaaacctga cgatcatggc gcagacgcgg
     1381 gcgatccggt tgctgatgga cggaactcgc tgcacaggcg tcgaggtcga ggggccggat
     1441 ggacgtcggg agatgcgggc cggtcaagtg attctctcgg cgggcgcaat ccactccccg
     1501 gcaatgttga tgcgggccgg gatcgggcca ggcgagcatc tcacgcagcg cggaatcgag
     1561 gtcgccgccg atcgggcggg cgtcgggcag aacctgatgg atcacccgat gatctcggtc
     1621 tcggccttca tgaagcgatc ggcccggctc gcgaaggcgc agcggcggca cattttcctc
     1681 ggcctgcgct ggtcgtccgg cctcgccgat tgcccaccgg ccgatatgta cgccgttgcc
     1741 tacaaccggg gcgcctggca tccggtcgga tggcgaatcg gcggcttcct gacctggatc
     1801 aacaagtcgt attcgcgcgg ctgggtgaag ctggccgatg cctcgcccga gcgcgagccc
     1861 gaggtccagt tcgacctgct cggcgatgag cgggatctcg gccggatgcg ggatgcggtc
     1921 aggcggatga tcggctactt cgatcacccg gccctcaagg cggtcgcggg cgagccgttc
     1981 ccgactagct acaccgagcg atacaagcgg cttgcggcgg tgaaccggcg caacctggcg
     2041 atcacgaccg ggcttgggtg gacgctcgat ggtccagctc aattgcggga gcggatgctg
     2101 cgcggcgtag tgaccgacgg cacctcttac gacgcaatcc tcgacgacga cacggcactc
     2161 ggcgattatc tgcgcgcgac cgcaaccggg atctggcacg cgagcgggac ctgccggatg
     2221 ggcgatccgc gcgatccaca ggcggtgacc gacggcaatg gccgggtgat cggcatcgag
     2281 gggctgcggg tctgtgacgc ctcgattatg ccgacgattc cctgcgcaaa cccgaacgtg
     2341 ccgatcatga tgatggccga acgaatcagc gacctgatcc tgaacgaggc gtaaggaggc
     2401 accggcgtgc gcttctcacg gatgatcacc gcggtcgatg cccatgccgg gggagagccg
     2461 ggccgagtca tcaccggcgg ggtcgggccg atcccgggtg actccgtctt cgcgcagatg
     2521 caattcctgg ccgaagaaca ggacgagatc cgtaagctga tgctgcggga gccacgcggc
     2581 tatccgggcc tctgctgcaa cctgctcgtg ccgcccacca atccgatggc cgacatgggc
     2641 ttcatcatca tggagcagat ggaatacccg gccatgtccg gctcgaacac gatctgcgtc
     2701 acgaccgtgc tgctcgagac cgggatcctg ccaatgaccg agccggtcac cgagttgacg
     2761 ctggagtcgc cggccgggct ggtcaaggtc cgggccgact gcgccaatgg caaggtgacc
     2821 cgcgtgacat tcgagaacgt gccgtcgttc gcgctgcacc tgaacgttcc ggtcgaggtg
     2881 ccgaatatcg gcacgatcaa ggtcgatgtt gcgtggggcg ggatgatgta cgtcctgttc
     2941 gagggttggc aggtcgggct gcacctgacg ccagacgagg gcgccgattg cgtcaagtac
     3001 ggcgagatgg tcaaggcagc cactcgcgag cagtacccgt cggtgcatcc cgagatcccc
     3061 gagctgactg gtccgacgat tgcgatgatc accggcaacc cgatgagcga gcatgcgcac
     3121 ggccggaata cggtcgtgat ctcgacgggc gacttcgact gggagaaccc atccacgtgg
     3181 accggcgcgc tcgaccgatc agcatgcgga accggtacaa gcgcgcggat ggcggtcctg
     3241 aatgccaagg gcaagctcgg cctgaaccgg gactacgttc acgagtcgat tctggggacg
     3301 acgttcatcg gtcgcctgat ccgcaagacg aaggtcggat cgtacgacgc ggttgtgccg
     3361 acaatcagcg gccaagcatg gatcaccggc atcgcacagt acctcctcga cccgaccgac
     3421 cccttccccg agggctaccg cgtcgccgac atctggggcg ggatttcagc cgtctgactt
     3481 ttcaaccgat tctcctaaag acccataacg agactcatcg cagaattaga tttgcttgat
     3541 tgtgaatcat taaggagtta tcccaaggaa tgcaggttcc tgcggctcag cctcagattg
     3601 acgaccgcgt gttgcgggag attcaccatc acctgcgcgc ggaagaacgt ctcctctggt
     3661 taggacagcc agccaaagat acgcctatga acgcggcaca aaaggtcaat gtaattggcg
     3721 gaagcgttgc tcttgccggt ttcatcgtca tcggtctgct taccagctac tgggggtttt
     3781 tcgccgaagt tgcgatcttg atctgcttcg gagccgggat cgcagctggg ctgatcgtct
     3841 ttactgtatc taacaagaag gtggccgaac gttactccac gtacgtgctt acagaccaac
     3901 gtgcctttag cttcatatac gacggtgaac ttgccgtagt aagttcctta ccgctgtcca
     3961 cacacaccaa gataagttcc cgaatcaaga ctgccacaac gggtagcttg ctattcaccc
     4021 gggctcaccg aaaagcgcct gatcttccca ggctgaccaa actgctacct gtaaacaagc
     4081 gatcaagtcg attgacgttt catggagtcc accagcccga atacgtgcag caagtcgcac
     4141 tttgggctgc cgctcatcac aacgaccttg gagcctaaga acagacagga cggctcatga
     4201 tgccttggcc agctctcggg ctggttcgtt tgtggggtcg ggtgcggcca ggatggcggc
     4261 gatgcgctct ggttcttggc tgatcaccgt aaggtaggcg cgggcggcgg agtcggggaa
     4321 gcgggcgcgg cgctcccagt cgcgcagggt cccgagcggg atccggtatc gccgcgagaa
     4381 ctccttttgg gtcagcttca gcttcctgcg gatgcgcttc ggatccggca gcttcttgat
     4441 cttctcccgg tcctcatcag tgaggaacgg ctcgaagtca gggtccatga gcgcgttgta
     4501 ttcaatatcc tcttctgtca tcgccctcag gcgtgcccaa tccgtgccgc cagtccgcat
     4561 cggtacgcat gtgccgtctg gcagctcctc gacccaggag ccgtcatcaa gtcggcgaac
     4621 tcgcacggta tcttcttgct tcgtcattgc tcgcgttcct tgccgagatg attctgatgc
     4681 agtcaccacg attcatcgca acgaccgtca ctagttggtc acccatcaac ccaacgcaca
     4741 ggaaccgcat ctcgccgtag tcgggattgg gactaacctc aatccggatt gacggatcgt
     4801 cgaatacctt gaccgcttcg aagaatgaga tcgagtgctt ctcgatgttg atttcattct
     4861 tgaccggatc ccattcgaaa ccgggcggaa attcctgccc tggaaaatac tgcactgcag
     4921 tacctctcgc aactgcgagc ctatggcgaa atgacgccgc gcctcaatga acgcggctga
     4981 tacccaaggt ttcgcaccga cactggcgaa tattatcagc ccgcgccggg acgattgttc
     5041 agcagccggt tcggacggtt tggttgagag aggcccagaa ggtgggaagg gatttgtagt
     5101 aggacggggt gtcatcgtcc gtgatccaga cgtagccggc gttgcgctgg cgggagagct
     5161 cgattgcgtc ctgccatgag ccggacggcg tcgagtggac gatgtgc
//
LOCUS       DATAOZ010000671         4137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1310352, whole genome shotgun sequence.
ACCESSION   DATAOZ010000671 DATAOZ010000000
VERSION     DATAOZ010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310352"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VHR64_16865"
     CDS             complement(<1..1174)
                     /locus_tag="VHR64_16865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX3304561.1"
                     /translation="MRRICVVGGGGYVGLGYAVALANLGHQVVGLDIDADRVAYLNQG
                     LSPIYEAGLPEMLRHNMEAGRLRFTSDYAQAVPGSELVFLCTGTPSLSDGEADLRQVR
                     AAAAAIATHLQPGTSTVIVNKSTMPVGTAEHVAGIMAEHVAPGIDFAIVSNPEFLREG
                     QVLHDILNPDRIVLGGDDEAALELVAELYRPLEAPILRMDHRSAELVKYAANAFLATK
                     ISFINDIARLCERMGADVTVVAEGIGADARIGPRFLKAGIGFGGSCFPKDVGALSRMA
                     EGAGLHPGLLRSVLEINRESQRRFVTKAEQLLAGLDQRTIAVLGLAFKEDTDDLRESP
                     AVTVVGMLMERGAIVRAHDPAALKHAATRLPGVTLCDDPYEAATGADAVALCTPWAD"
     gene            complement(1171..1542)
                     /locus_tag="VHR64_16870"
     CDS             complement(1171..1542)
                     /locus_tag="VHR64_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304562.1"
                     /translation="MNLLEATLDAIDWSVFATADGSPLTDSAERDLRNYYRQKWSDIG
                     PLFLAELLSTEFMTEQRARGDITFSEALLRLGREEPELWQEIRQFFRRKEMVTALLAA
                     ADDPTAIRAETDELDEEDLWE"
     gene            complement(1695..2669)
                     /gene="prmA"
                     /locus_tag="VHR64_16875"
     CDS             complement(1695..2669)
                     /gene="prmA"
                     /locus_tag="VHR64_16875"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX3304563.1"
                     /translation="MKADAPSGEWIELAVTCEAEAVESVSELFARHGFNQGVVIEEPF
                     TQDPDGDNFAVDPSRPVLVRTYLSAIDVKPETIEDIRHGIWHLGQIRSITPLQVTRRA
                     EEDWANAWKAHFRVHRVGQHVLVKAPWHDYAPAEGEVVVELDPGMAFGTGTHPSTQLC
                     MVALEETLRPGDRVLDVGVGSGILSIAAALLGASTVDAVDIEPVAVRATRENAERNRV
                     ASIVHVESGSMGDDQPFQGEYDLVVANIIARILIDLAPALTRSVATDGTLILGGIIDV
                     KEQAVHDAFLATGLMLTRREQQEDWVALVLHKPAICPAADELAPILRA"
     gene            complement(2666..3640)
                     /locus_tag="VHR64_16880"
     CDS             complement(2666..3640)
                     /locus_tag="VHR64_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX3304564.1"
                     /translation="MDIILPVAGLGTRLRPQTWTKPKPLVSLAGKPMLAHALDQVLPV
                     GPEQIIFITGFLGDQIEIWARESLDVPVAFVTQPEMRGQTDAILRTRGLVERDALILF
                     PDMLFEAEFAGLEDVDADVVMFTKEVEDPSALGIAVVEQDRIVRLIEKPQEPISNLAV
                     IGIYYVRNMEDLYTAIEEQMHRGMALKNEYYIADAIQIMIDRGARVISRPVTTWEDCG
                     NADALLSSNRSHLERHNPTVGQRDGSVIIAPSYVAEDAIIVNSVIGPFASIAGGAVIR
                     GSIVRDSIIESGVEICGATLEQSIVGRRVSVHGFRGRLNVGDDAVVGL"
     gene            complement(3766..>4137)
                     /locus_tag="VHR64_16885"
     CDS             complement(3766..>4137)
                     /locus_tag="VHR64_16885"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX3304565.1"
                     /translation="VPSSLAASMGFVAAAVVSFAIIGFAARLLLSPLRVLGADTPVGW
                     VNGILGLLPGAVRGLAMAALLLLFLSAFPAETGVRTTLAGSRLARPILTTGQQALHDG
                     LTWAGIDPRSLGVLYQTVSPR"
BASE COUNT          833 a         1269 c         1277 g          758 t
ORIGIN      
        1 agtctgccca gggtgtgcac agggccaccg cgtccgcccc ggtagccgct tcgtacgggt
       61 catcgcaaag cgtcacgccc ggcaggcgcg ttgccgcatg cttgagcgcg gcgggatcgt
      121 gggctcggac gatggctcca cgctccatca acatgccgac gacggtgacc gcgggagatt
      181 cgcgcaggtc atcggtgtct tctttgaagg cgagacccaa gaccgcaata gtgcgctgat
      241 cgagtccagc gagaagctgt tcggccttcg tcacaaagcg gcgctgtgac tcgcggttga
      301 tctcgagcac tgaccgcaag agtcccggat ggagaccggc gccttcggcc atgcggctca
      361 gagcaccgac gtctttgggg aaacaggaac cgccgaaacc aattcctgct ttgagaaagc
      421 gaggtccgat ccgagcatct gcgccaatac cctccgcaac gaccgtgaca tcggcgccca
      481 tccgctcaca gagccgagcg atgtcattga tgaacgagat cttggttgcc aggaaggcat
      541 tggcggcgta tttgaccagt tcggccgaac ggtggtccat gcgcagaatc ggagcctcca
      601 ggggcctata gagctccgcc accaattcta gtgccgcctc gtcgtcgcca cccagcacga
      661 tccgatctgg gttcaggatg tcgtgcaaga cctggccctc acgcaagaat tcggggttgc
      721 tgacgatggc aaagtcaatc cctggggcaa cgtgctcggc catgatcccc gcgacgtgct
      781 cggccgtgcc gaccggcatg gtgctcttgt tgacaatcac cgtcgaggtc cccggctgaa
      841 gatgggtcgc gatcgccgcc gccgctgccc ttacctggcg taggtcagct tcgccgtcgg
      901 agagggaggg tgtgccggtg cagaggaaga cgagctcgct ccctggcacc gcctgggcgt
      961 aatcggaggt gaagcgaagg cgccccgcct ccatgttgtg ccgcaacatc tcggggagac
     1021 cggcttcgta gatcgggctc agaccctgat tgaggtaggc aacacggtcg gcatcaatat
     1081 cgaggccgac gacctgatgt cccaggttgg ccagagccac ggcgtagcca agtccgacgt
     1141 acccgccccc acccacgaca catatgcgcc tcattcccac agatcctcct cgtccaactc
     1201 gtcagtctcg gcacggattg cggttggatc gtccgcggcg gcgagtagcg ctgtcaccat
     1261 ctccttgcgg cgaaaaaact ggcggatctc ctgccagagc tccggctctt cgcggccgag
     1321 gcgaagcaac gcctcggaaa aggtgatgtc gccccttgcg cgctgctccg tcatgaactc
     1381 ggtcgaaagt agctcggcca gaaacaaggg accaatgtcg gaccactttt gccgatagta
     1441 gtttctcagg tcgcgttcgg cactgtccgt gaggggagag ccatcagccg tggcaaagac
     1501 cgaccagtcg atagcgtcga gtgtggcttc caggagattc acgcgctctc ctgaccggac
     1561 tcggtccaca agtgagtcaa tgacggcgcg atcaaggggt agctcatgag gtgtgtcggg
     1621 caacggcggg ggcgcgtccg gaaaggcccg ggccccgtgc gcgatcacat cgtccgagct
     1681 gtcaagcgag gcgctcatgc gcggagtata ggggccagtt cgtcagcggc cgggcagatg
     1741 gcgggcttgt gcagcacgag cgctacccaa tcttcttgct gctcgcgacg tgtcagcatc
     1801 aatccagtgg ctaggaatgc gtcatgaacg gcctgctctt tgacatcgat gatcccgccc
     1861 aggatgagcg ttccgtctgt ggccacggag cgggtcagag cgggcgcaag gtcgatcagg
     1921 atacgggcaa tgatgttggc aacgacgagg tcgtactcgc cttgaaatgg ctgatcgtcg
     1981 cccatgctgc cagattcgac gtgaacgatc gacgcgactc gattgcgttc cgcgttttcg
     2041 cgtgtagcac gcacggcaac cggctcgata tcgaccgcat cgacggtgct ggcgccgagc
     2101 agggcagcgg cgatactcaa gatgccggac cccacaccga cgtcgagtac acgatcaccg
     2161 ggtcgcagtg tctcttccag ggcgaccata cagagttggg tcgaggggtg ggtgccggta
     2221 ccaaaggcca taccgggatc gagctcgacg acgacttcgc cttccgcggg cgcgtaatcg
     2281 tgccagggcg ctttgacgag aacgtgttga ccgacgcgat gcacccggaa atgagccttc
     2341 caggcattgg cccagtcctc ttcggcacgc cgagtgacct ggagcggcgt aatggaacgg
     2401 atctggccaa ggtgccagat gccatgccgg atgtcttcga tcgtttcggg tttgacgtct
     2461 attgcactga gatacgtgcg aaccaagact ggtcgtgaag gatcgacggc gaagttatcg
     2521 ccgtcagggt cctgggtgaa cggttcctca atcaccacac cctgattgaa accgtggcgg
     2581 gcaaacaatt ccgagacaga ctcgacggcc tccgcctcgc acgtcaccgc aagctcgatc
     2641 cactcgccgc tcggcgcgtc tgctttcata aaccaacgac ggcgtcgtcg ccgacgttga
     2701 gccgccctcg gaaaccgtgc acgctgactc gacgcccaac gatcgattgc tcgagcgtgg
     2761 ccccacagat ctcgacacct gactcaatga tcgagtcgcg gacgatgctg ccccgaatca
     2821 ccgctccgcc tgcgatgctg gcaaatggcc cgatgacaga gttgacgatg atggcgtctt
     2881 cggcaacata cgacggggca atgatgactg acccgtcgcg ttgcccaacg gttggattgt
     2941 gccgttcaag gtgagatcga ttggagctca gcagagcgtc ggcattacca cagtcttccc
     3001 atgtcgtcac cggcctggaa atgactctcg cgccacggtc gatcatgatt tggatagcgt
     3061 ccgcgatgta atactcgttc ttgagcgcca tgccgcgatg catctgctct tcaatggcgg
     3121 tatagagatc ctccatgttc cgcacgtagt agatgccgat gaccgctaaa ttggagatgg
     3181 gttcttgcgg cttttcaatg agccgcacga tgcggtcttg ttcgacgacc gcaatcccta
     3241 aggccgacgg atcctcgacc tctttcgtga acatcacgac gtcggcgtcg acatcctcga
     3301 gaccagcgaa ctccgcttcg aagagcatgt cggggaagag gatcagtgca tcacgctcga
     3361 ccaaccctcg agtgcgaagg atcgcatccg tctggccgcg catttcgggt tgagtgacaa
     3421 acgcgacggg aacgtcgagt gactctcggg cccagatctc gatctggtcg ccgaggaaac
     3481 cggtgatgaa aatgatctgt tccggcccta cgggcaggac ctgatcgagg gcatgcgcca
     3541 gcatcggctt gccagcaaga ctgaccagcg gtttcggctt ggtccaagtt tgcgggcgca
     3601 accgggtgcc gagccccgcg accggcaaaa tgatgtccac actctcctcc ggcggtattc
     3661 gctaccaacg acggcttgcg gccgctccaa gccgacagtt ggtcgatttg cgggcactct
     3721 agcaaagcgc ggtccaacaa accgtgccct cgtggcacaa ggatctcatc tcggactgac
     3781 cgtctgatag agcacgccga gcgaacgcgg atcgatacca gcccatgtga ggccgtcatg
     3841 caacgcttgc tggccggtcg tcagtatggg gcgtgccagg cgggagccgg ccaacgtggt
     3901 gcgcacgccg gtctccgccg gaaatgcgga caggaaaagc agcaggagtg cagccattgc
     3961 caggcctcgc acggcaccgg gcagtagtcc caggatccca ttcacccatc ccaccggtgt
     4021 gtccgcgccg agaactctga gaggagagag aagcaatcgg gcggcaaaac cgataatcgc
     4081 gaagctcacc accgctgcgg caacaaaacc catactggcg gcaagcgatg atggtac
//
LOCUS       DATAOZ010000672         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_2629829, whole genome shotgun sequence.
ACCESSION   DATAOZ010000672 DATAOZ010000000
VERSION     DATAOZ010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2629829"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VHR64_16890"
     CDS             <1..472
                     /locus_tag="VHR64_16890"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX3304566.1"
                     /translation="APAESSPSPAIGQVNRGATVEVIARDRGWVRVRLEGWVREADLI
                     VADSSLRPLSAADIRSDPVSSQGKLVRWRVQAISMQTADALRTGLKSGEQYLLALGPG
                     AEKTLVYLVVPPALMPSARNLPAMADIVVIARVRNGRSEPAGVPILELQSLTRQ"
     gene            473..1162
                     /locus_tag="VHR64_16895"
     CDS             473..1162
                     /locus_tag="VHR64_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304567.1"
                     /translation="MAKLTIDSDKPYDPLEGPGSRRDPAKGREGDRRRAGWRDFRRAY
                     PGFIFTLLIALIAIISLDGYLIVKRRAYTAEVQRLRSAMTDAERSKTDAIVQAEQDKA
                     RIALELARRQAKLEKGLHLAVAVDSGRIYLEREGAILREMAAQFGPEKGVTPGTDSVP
                     VVVPRGQRTVAKLEENRIVLEGGSAIEAAHTDVVAADTTPIPPGDVRIGLTDLKAIMP
                     NLTAGMRVYFY"
     gene            1197..>1630
                     /locus_tag="VHR64_16900"
     CDS             1197..>1630
                     /locus_tag="VHR64_16900"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX3304568.1"
                     /translation="MMGFVNTMTHGGKWVWITLLGFLGATAASAMLVTKTADLRYQRD
                     VNRMVFNDNLGVLQEVKAKLGMTTDSLNRLVSTSPAAVAPQQPYIVVSIEERQLWYKK
                     GNEVLFHTQVATGSGKTLVSGAGGKQWKFETPRGRLTVQAKE"
BASE COUNT          336 a          474 c          532 g          288 t
ORIGIN      
        1 ggcaccggcg gagagctcgc catcgccggc gattgggcaa gtgaatcgtg gagccaccgt
       61 ggaggtgatc gcgcgcgacc gcggctgggt gcgagttcgt ctcgaaggtt gggtgcgtga
      121 ggccgatctc atcgtcgccg atagctcgct gcgacccctt agtgcagcgg acatcagaag
      181 cgatccggtc tcttcgcagg gcaagctcgt tcgctggcgc gtgcaggcga tttcaatgca
      241 gaccgcagac gctctgcgca ccggtctcaa gtccggcgag cagtacctgc tcgctcttgg
      301 tccgggcgcc gaaaaaactt tggtctatct tgttgtgccg cctgcactaa tgcccagcgc
      361 ccggaatctt ccggcgatgg ctgacatcgt tgttattgcg cgcgtacgca acggccgcag
      421 cgagccggcc ggagttccca ttctcgagct tcaaagtcta acccgacaat aaatggcaaa
      481 gctgacgatc gattctgaca agccttacga tccgctcgag ggccctggca gccgccgcga
      541 tcccgcaaaa ggacgggaag gcgaccggcg ccgggctgga tggcgtgatt ttcggagggc
      601 ctacccggga ttcattttca cgctgctgat tgcgctcatc gcgattattt ccctcgacgg
      661 gtacttgatc gtcaagagaa gggcgtacac cgccgaagtg cagcggctac ggagcgccat
      721 gaccgacgcc gagcgatcga aaactgacgc gatagtccag gcggagcagg acaaggctcg
      781 catcgcgctg gagcttgcgc gccggcaggc caaactcgag aaagggctcc atcttgctgt
      841 tgcagttgac agcgggcgca tctacctgga gcgggaaggc gcgattctaa gagagatggc
      901 cgcccagttc ggacccgaaa agggagtgac tccgggaacc gattcggtcc cggtggtcgt
      961 cccgcgcgga cagcggaccg tcgcgaagct ggaggagaac aggatcgtcc ttgaaggggg
     1021 gtcggcgatt gaggcagcgc acactgacgt ggtcgctgcc gacaccactc cgattcctcc
     1081 aggcgatgtg cgaatcggac ttactgacct gaaggcgatc atgccgaatc tgactgccgg
     1141 catgcgggta tacttttatt gactcgggca accagtccgg gtctcaccag aggtaaatga
     1201 tgggtttcgt caacacaatg acgcacggcg gcaagtgggt ctggataacg ctgctcggat
     1261 tcctcggagc aacggcggca agcgcgatgc tcgtcacgaa gacggccgac ctccgctatc
     1321 agcgcgatgt gaaccgcatg gtgttcaacg acaacctggg cgtgcttcag gaagtgaagg
     1381 cgaagctcgg catgacgacc gacagcctta accgactggt ctcgacgagt ccggcggcag
     1441 tggccccgca acagccgtac atcgtcgtca gcatcgagga gcgccagctc tggtacaaga
     1501 agggaaacga ggttctgttc cacacccagg ttgccaccgg aagcgggaag acgctggtct
     1561 ccggtgcggg tggcaagcaa tggaagttcg aaacgccgcg cggtcgcctg acggtccagg
     1621 caaaagagga
//
LOCUS       DATAOZ010000673         6451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4467773, whole genome shotgun sequence.
ACCESSION   DATAOZ010000673 DATAOZ010000000
VERSION     DATAOZ010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4467773"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VHR64_16905"
     CDS             complement(<1..911)
                     /locus_tag="VHR64_16905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3304569.1"
                     /translation="MRKIRTSSWSTLLVLLLVAIIVGACGGDSSPKEPTNTPAPMPTS
                     AATVTQSVVSPVSSSQASPVGSPNASPIASPVGSPVALTSGASTMTRAEFKSALLSNF
                     PMDAPASQGGTIVLGSSSDISTTNPLLASDSTTINLLGQVFEPLLGASPIDGRPIPAL
                     ADSWTVSDDGLTYTFTLNPDAKWHDGVDVTADDVAFSFDAVLNPNLNSQYRSQVREVV
                     KSYRVVDPDTFEITATDRFVTFLYNAPASVFIVPKHIWESVPVEQWSFDGGSTGEDVS
                     RIVGSGPFVFKSWEQGQRVTLARNPNH"
     gene            1201..2313
                     /gene="ald"
                     /locus_tag="VHR64_16910"
     CDS             1201..2313
                     /gene="ald"
                     /locus_tag="VHR64_16910"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX3304570.1"
                     /translation="MIVGIPREIKDRENRVSTTPAGVAEYVARGHSVLVECDAGTGSG
                     FANTEFEKAGAKLVNSHEAVFAESDMIVKVKEPIPSEYDLLKPNQLLFTYLHLAAEEK
                     LTRALMEHKVQAVAYETVQLANGSLPLLTPMSEIAGRMSIQVGAHYLERTYGGEGLLL
                     GGVAGVPGAEVVIVGGGVVGTNAAQMALGMGANVTIIDKNVERLRFLDQVLHGRSHTL
                     ASNSQNLHDWIGRADFVVGGVLLPGAKAPKLVSKEMIKSMKTGSVVVDVAIDQGGCFE
                     TSKPTSHSDPVYDVSGVIHYCVTNMPGAVARTSTLALSNVTLPYGLALADKGLADAAR
                     AFPELAKGINVLNGHVTYQAVADAFGLPYQPWDVAA"
     gene            2384..3292
                     /gene="xerA"
                     /locus_tag="VHR64_16915"
     CDS             2384..3292
                     /gene="xerA"
                     /locus_tag="VHR64_16915"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HEX3304571.1"
                     /translation="MRSAVQQFLTALEADRGFAANTIAAYRNDLEQFCTYVQNPPEED
                     HIPAISQWSELTEAHLSTYILHLRSREYAASTIARKTAALKSFSAYLVERGVTRIDAA
                     EKLSAPRVEKYTPRSISPSEIDQLLAQTARAAAENRPEGLRDQAMLETLYSTGMRVSE
                     LVALNLPDVDLERRVAKCTGKGGRQRSVPLRTSAINALRNYLEHGRPRIAPNEEQALF
                     VNHRGSRLTRQGFWLILKAYARQAGIQDITPHTLRHSFATHALRHGYELRDVQQMLGH
                     VSISTTQVYRRIATPAPAAEPAATGE"
     gene            3345..4727
                     /locus_tag="VHR64_16920"
     CDS             3345..4727
                     /locus_tag="VHR64_16920"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX3304572.1"
                     /translation="MDKIVALAKRRGFVFPGSDIYGGLANTWDYGPLGVELKNNIKQL
                     WWRTFIHRRRDMVGLDAGILMHPRVWEASGHVVNFNDPLVDCKTCKSRFRADHLIEEK
                     LGIHAEGMSPDKMSEILREANLPCPVCGNRTLTDARQFNMMFRTTIGPVSESGTEVYL
                     RPETAQAIFVQYKNILSTSRVKIPFGVGQIGKAFRNEITPGNFIFRDIEFEQMEIEYF
                     VRPDDGATKFEEWLEAMQAWVNQIGLNPDHVRLREHATDELSHYSSRTVDFEYLFPEP
                     MGWRELYGLANRTDFDLGRHQEYSGEDLRYFEQESGERYLPHVIEPTFGVDRTILVVL
                     LDAYDEEETVDVNGKPDIRTVLRLAPALAPYKAAILPLMKKPELTDVARSLFQEIQRD
                     TDFVVDYDETANIGKRYRRQDEIGTPYCFTVDYESLDDSCVTVRHRDSMEQERVAIAD
                     VRSFLQDAIR"
     gene            4748..5413
                     /locus_tag="VHR64_16925"
     CDS             4748..5413
                     /locus_tag="VHR64_16925"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX3304573.1"
                     /translation="MTEKQVQTRAARRQAMVKERRNVHRQRYDRNKREMMIIKGISIA
                     LALIVLGALGFAAFNWAQDRNLNRAPEGVKTFQYAQGNHIEGNIDYSAQAGYQGELPP
                     AGGAHNSTAQQCDVYTQPIRQENAIHSLEHGAVWITYQPDLAADQVTNLDDIVKGDPY
                     LMVSPYPGSPAPIVLTAWNHQLQLQSYDKDTIEQFIRSYKNKQGVTPEFGASCAGTTA
                     TAP"
     gene            complement(5417..6370)
                     /locus_tag="VHR64_16930"
     CDS             complement(5417..6370)
                     /locus_tag="VHR64_16930"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX3304574.1"
                     /translation="MNELSDARIEEIRAGLLAWYAANHRDLPWRHTRDPYRVLVSEVM
                     LQQTQVDRVIPYYGRWLTQFPTVEALADAPTADVITAWSGLGYNRRAVYLQKTARAVV
                     DEHGGVFPSTREELLNLPGVGPYTAGAIAAFAFEQDVAFMDTNIRRVVHRLIFGSDVP
                     ARQATERDILAAAERLVPEGAGWVWNQAMIEFGALQCTQRRPACVICPLQRACSAFPA
                     IQSDIAELPAGVRLKNEANFHGSNRQFRGRVVEELRRSSSATLAELGPKVKHDFAEQD
                     LPWLYGVVSGLARDGLVAIREPAAEYDPDSPAPGIVVALPD"
BASE COUNT         1287 a         2022 c         2032 g         1110 t
ORIGIN      
        1 taatggttcg gattcctggc gagcgtcacg cgctggccct gctcccagga tttgaagacg
       61 aacggcccgg agccgacgat ccgggagaca tcctcgccgg tactccctcc atcgaagctc
      121 cactgctcga ccggcaccga ctcccagatg tgcttcggca cgatgaaaac cgatgccggg
      181 gcgttgtaca ggaaggtgac gaagcggtcg gtcgcggtga tctcgaaggt gtccggatcg
      241 acgacgcggt acgacttgac gacctcgcgc acctgagacc ggtactggct gttgagattc
      301 gggttgagca ccgcatcgaa gctgaaggcc acgtcatcgg ccgtgacatc gactccgtcg
      361 tgccacttcg catccgggtt gagcgtgaag gtgtaggtca gaccgtcatc gctgaccgtc
      421 cagctatccg ccagcgccgg gatcggccgg ccatcgatcg gactcgcgcc gagcagcggc
      481 tcgaagacct ggccgagcag gttgatggtc gtcgagtcgc tggccagcag cggattcgtc
      541 gtgctgatgt cactgctcga gccgaggacg atggtgccgc cctggctggc tggcgcgtcc
      601 atcgggaagt tcgagagcag cgcgctcttg aactcggcgc gggtcatcgt cgatgcgccg
      661 gacgtcagcg caacgggcga tccgaccggt gaggcaatcg gcgacgcatt cggcgaaccg
      721 accggcgaag cctgagaact ggacacgggc gacacgacgg actgcgtcac cgtggccgcg
      781 ctggtcggca tcggcgccgg cgtgttggtt ggctccttgg gcgacgagtc gccaccgcac
      841 gcgccgacga tgatggcgac cagcagcaag acgagcagcg tgctccatga agaggttcga
      901 attttgcgca acgtcattcc ctgcccggtc ggctcaaccg catcaattca atctgtccgg
      961 ggaatcgtac ccaagagttg caaacgactg aaaaccgtgc ccgaaagggg ctccattttg
     1021 cccgattcga gacaagtaaa gagtgacttt atggtagagt tgggcactgc tggatcaacg
     1081 agtttcgggg tcgaaaacca ttcacgcagt tcacgttttg ttgccgcctg gaacgcagcg
     1141 aacctgcccc aggcatcgat ctatgtgccc gttcaccgtt gaacgcgagg tagacacggc
     1201 atgattgtcg ggattcccag ggaaatcaag gaccgcgaga accgtgtttc gacgacgccc
     1261 gccggtgttg ccgaatacgt tgcccgaggc cattccgtgc tggtcgagtg cgatgcgggg
     1321 accggcagtg gcttcgcgaa taccgaattc gagaaggccg gcgcaaagct ggtcaactcg
     1381 cacgaggcgg tcttcgcgga gtcggacatg atcgtcaagg tcaaggaacc gatcccgagc
     1441 gagtacgacc tgctcaagcc gaaccagctc ctcttcacct atctgcacct cgcggcagaa
     1501 gagaagctga cccgcgcgct gatggagcac aaggtccagg cggtagccta cgagacggtc
     1561 cagctggcca acgggtcgct gccactgttg accccgatga gtgagatcgc gggccggatg
     1621 tcgatacagg tcggcgcgca ctacctggag cgcacctatg gcggcgaagg gctgttgctt
     1681 ggcggcgtgg ccggggtgcc gggcgccgag gtcgtcatcg tcggcggcgg cgtggtcggc
     1741 acgaacgcgg cgcagatggc cctcggcatg ggcgccaatg tgacgatcat cgacaagaac
     1801 gtcgaacgcc tgcgctttct cgatcaggtg ctgcatggcc gcagtcacac gctggcatcc
     1861 aactcccaga atctccacga ctggatcggc cgggcggatt tcgtggtcgg cggcgtgctg
     1921 ctgccgggcg caaaggcccc gaaactggtc agcaaggaga tgatcaagag catgaagacc
     1981 ggctcggtcg tggtcgatgt tgcgatcgac cagggcggct gcttcgagac gtcgaagccg
     2041 acgtcgcaca gcgacccggt ctatgacgtc agcggcgtca ttcactattg cgttacgaat
     2101 atgccgggcg ccgtggcccg gacgagtaca ctcgcacttt cgaatgtgac cctgccgtat
     2161 gggctggcgc tggccgacaa gggcctcgcg gatgcggcac gggcatttcc ggagcttgcg
     2221 aaggggatca acgtcctcaa tggccatgtg acataccagg ccgtcgccga cgcgtttggt
     2281 ctgccatatc agccatggga cgtcgccgcc tgatcacgca agcaaccggc aacattcgcc
     2341 ttcgtaccgg gggtgaaggg gaaagggaca cgtggacgga gatttgaggt ccgcagttca
     2401 acagtttttg accgcgctgg aggctgaccg cggttttgcg gcgaacacga ttgccgccta
     2461 tcgcaatgat ctggagcagt tctgcaccta cgtgcagaat ccgccggagg aggaccatat
     2521 tcccgccatc agccagtgga gcgagctgac tgaggcgcat ctcagtacct acatcctcca
     2581 cctgcgcagc cgggaatatg ccgcctcgac gatcgcgcgg aagaccgcgg cgctcaagtc
     2641 gttcagcgcc taccttgtcg agcgcggcgt cacacgcatc gacgctgccg agaagctgag
     2701 cgcgccccgg gtcgaaaaat acacgccacg ctcgatcagc ccgtccgaga tcgatcaact
     2761 cctggcgcag acggcccggg ccgcggccga aaaccgtccc gagggcctgc gtgaccaggc
     2821 tatgctcgag acgctctatt cgaccggaat gcgcgtcagc gagctggtcg cgctcaacct
     2881 gccggacgtc gatctggaac ggcgcgtcgc caagtgcacc ggcaagggcg gtcgccagcg
     2941 gtcggtcccc ctgcggacca gcgcgatcaa cgcgttgcgc aactatctgg agcacggccg
     3001 gccccggatc gccccgaatg aggagcaggc gctgttcgtc aatcatcgtg gtagccgcct
     3061 gaccaggcag ggcttctggc tgatcctcaa ggcgtacgcg cgccaggcgg gcatccagga
     3121 catcaccccg cacacgctgc gccattcgtt cgcgacccac gccctgcgcc acggctatga
     3181 gctgcgcgac gttcagcaga tgctcggaca cgttagcatc tcgaccacgc aggtctatcg
     3241 ccgcatcgcc acgccggcgc ccgccgcgga gccggcggcc acgggcgaat aggccaaccc
     3301 ggagagtctc cctgcagatg agtcagtcgg tacgtgcttc gtccatggac aaaatcgtcg
     3361 cgctcgccaa gcgacgcgga ttcgtctttc ccggatccga tatctacggc ggcctcgcca
     3421 atacgtggga ttacgggccg ctcggcgtcg agctgaaaaa caacatcaag cagctctggt
     3481 ggcgaacctt catccaccgc cgccgagaca tggtcgggct cgatgccggg atcctgatgc
     3541 accctcgcgt ctgggaggca tccgggcacg tcgtcaactt caacgatccc ctggtcgact
     3601 gcaagacatg caagagccgt ttccgcgcgg atcacctgat cgaggagaag ctcggcatcc
     3661 acgccgaagg aatgtcgccc gacaagatgt cggagatcct gcgagaggcg aatttgccct
     3721 gcccggtttg cggcaaccgc acgctgactg atgcccgcca attcaacatg atgttccgaa
     3781 ccacgatcgg cccggtcagc gagagtggca ccgaggtcta cctgcgcccc gagaccgcgc
     3841 aggcgatctt cgtccagtac aagaacatcc tgtcgacctc gcgagtcaag atcccgttcg
     3901 gcgtcggcca aatcggcaag gcgttccgga acgagatcac gccgggcaac ttcatcttcc
     3961 gtgacatcga attcgagcag atggagatcg agtacttcgt ccggccggat gacggcgcga
     4021 caaagttcga ggagtggctc gaggcgatgc aggcctgggt caatcagatc ggactcaacc
     4081 cggatcatgt tcgcctccgc gagcacgcca cggacgagct gtcgcactat tcgagccgga
     4141 ccgtcgactt cgagtatctg ttcccggagc cgatgggctg gagggagctc tacgggctcg
     4201 ccaaccggac cgatttcgac ctcggccggc accaggagta cagcggcgaa gatctccgct
     4261 atttcgagca ggaaagcggc gagcggtatc tgccccacgt gatcgagccg accttcggtg
     4321 tcgaccgcac catccttgtc gtgctgctcg atgcctacga tgaggaagag acggtcgacg
     4381 tcaacggcaa gccggacata cgcacggtcc tccggctcgc accggcactg gcgccataca
     4441 aggcggcgat tctcccgctc atgaagaagc cggaactgac cgacgtggca cgctcgctct
     4501 ttcaggagat ccagcgggac actgatttcg ttgttgacta tgacgaaacc gcgaatatcg
     4561 gaaagcggta ccggcggcag gatgagattg gcacgcccta ttgtttcact gtggattacg
     4621 agtcacttga cgactcatgt gtaaccgttc gtcaccggga ctcaatggag caagagcggg
     4681 tagcgatcgc cgatgtgcgc tcgttcctgc aggatgcaat caggtagagg catagggaaa
     4741 ccagtaaatg actgagaaac aggtccagac tcgcgcggca aggcgccagg ccatggtcaa
     4801 ggaacgccga aacgtccatc ggcagcgata tgaccgcaac aagcgcgaaa tgatgattat
     4861 caagggcatc tcgatcgcgc ttgcgctgat cgtgctcggc gcactcgggt tcgccgcgtt
     4921 caactgggcg caggaccgga atctcaaccg ggcgccagag ggtgtaaaga ccttccagta
     4981 cgcgcagggg aaccatatcg agggcaacat cgactactcg gcgcaggccg gctaccaggg
     5041 cgagctccca ccggccggcg gcgcgcacaa cagcaccgcg cagcagtgcg atgtctacac
     5101 gcaaccgatc cggcaggaga atgcgatcca ctcgctcgaa cacggcgcgg tctggatcac
     5161 gtatcaaccc gatttggcgg ccgatcaggt cacgaatctg gacgatatcg tcaagggcga
     5221 tccgtacctg atggtcagtc cctatcctgg gtcacccgca ccgattgtgc tgaccgcctg
     5281 gaaccaccag cttcagttgc agtcctatga caaggacacg atcgagcagt tcattcgcag
     5341 ctacaagaac aagcaaggcg ttacacccga gttcggcgcg tcctgcgccg gcacgacggc
     5401 tactgcgccc taagcatcaa tcgggcagcg caacgacaat gccaggagcc ggactgtcgg
     5461 gatcgtactc ggcggccggt tccctgattg caaccagacc atcccgagcg aggccggaaa
     5521 ccacgccata gagccagggc aaatcctgct cggcgaagtc gtgtttgact ttcgggccca
     5581 gctccgccag cgtcgcgctg gatgatcggc gcaattcctc aacgacccga ccccgaaact
     5641 gccgatttga gccgtggaag tttgcttcgt tcttcaaccg cactccggcc ggcagctccg
     5701 cgatgtcgga ctgaattgcc gggaaggccg agcacgcgcg ttggagcggg cagatcacgc
     5761 aggccggtcg ccgttgggtg cattggagcg ccccgaactc gatcattgcc tggttccaga
     5821 cccagccagc tccctccggc accagtcgtt cggctgcagc cagaatgtct cgctcggtcg
     5881 cctggcgggc tggaacgtcg ctgccgaaga tgagccggtg caccacgcgc cggatgttcg
     5941 tgtccatgaa ggcgacatcc tgctcgaagg cgaaggccgc aatcgccccg gccgtatatg
     6001 gcccgacccc cggcagattc agcagctcct cgcgagtgct ggggaagact ccgccgtgct
     6061 cgtcaacgac cgcgcgggcg gtcttctgca ggtacacggc ccggcggtta tagccgagtc
     6121 cggaccaggc cgtgatcaca tcggccgtcg gggcatcggc gagcgcttcg accgtcggga
     6181 actgtgtcag ccaacgcccg tagtagggga taacgcgatc gacctgcgtc tgctgaagca
     6241 tgacctcgga gacgaggacg cggtacgggt cgcgggtgtg gcgccagggc agatcccggt
     6301 ggttggcggc ataccaggcc agcagcccgg cccggatttc ctcgatcctg gcgtcagaaa
     6361 gctcattcat ccacaaagca tagcccgacc tacccttgcc agaatgacaa ggtttcgcta
     6421 tgtttgtacg acctgttcgt acaccggtgc c
//
LOCUS       DATAOZ010000674         4894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_6837988, whole genome shotgun sequence.
ACCESSION   DATAOZ010000674 DATAOZ010000000
VERSION     DATAOZ010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6837988"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VHR64_16935"
     CDS             <1..245
                     /locus_tag="VHR64_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304575.1"
                     /translation="ESLGALAIHVRNERELRQALEQAKQADRTTLISIAVPIDVQVPG
                     FESWWDVPVAEVTDQPSVEAARDAYEEKRKKQRVFI"
     gene            439..1437
                     /gene="iolG"
                     /locus_tag="VHR64_16940"
     CDS             439..1437
                     /gene="iolG"
                     /locus_tag="VHR64_16940"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEX3304576.1"
                     /translation="MRADLTFGVIGLGRMGMLHATNLRGGIAGARLKVAAVSPEDRAR
                     LEAEGSAPAPLVDVDDLIDDPEIQAVAVVSPTASHREHIERCAAAGKAVFTEKPVAST
                     LDDTLATADAVRKSGIPFQIGFQRRYDPSYARARELIEQGAIGSVEMFRGISCDGIPP
                     VSYLRTSGGLFRDLGVHDIDAARFLTGEEITEVSATGAILIEPALADFDDVDYGIVTL
                     RFANGALGVIQNCWRAPYGYDIRAEVHGSKGKVIADLDAKEPTALYTDRAFSFRRHHL
                     FVERFADAYRLELQAFVDAVHADTAPTPGIEDGVRAFVVADAATRSRIENRWIGVS"
     gene            complement(1552..2199)
                     /locus_tag="VHR64_16945"
     CDS             complement(1552..2199)
                     /locus_tag="VHR64_16945"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX3304577.1"
                     /translation="MEDRAKSTMLQSAPWRRGIPWYIVGIEGGLLALIGIYALVQPDK
                     ARDIARQLIGAILLVTAAIEAYASFRNPTHPAAPYRLFRSGVGVAVGVIVILEPVSDY
                     LDADASRFILGLGLIGYGLIGLAATVLAREESGIRIGSLITGGLSIVLGILFLTGNTD
                     NSSTRVNIIGAVLIVFGVLLLAYAFYLYRLLSKQPSGTDTEEAPPPSFAGTASGA"
     gene            complement(2257..2805)
                     /locus_tag="VHR64_16950"
     CDS             complement(2257..2805)
                     /locus_tag="VHR64_16950"
                     /inference="COORDINATES: protein motif:HMM:NF016413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEX3304578.1"
                     /translation="MRLFRHKDEEEAGGIATAMTPGHKRYKMKERMFSFGDDYVIEDE
                     EGNRAFKVDGKMLRLRSTLVIRDSEGHEVAKIQEKWMRVKDSMEIEGPNGQRIAMVKK
                     ALYTPVRQRFAINVTDGPDLDADGNVVNHEYEIKRDGFTVAVVSKKWFRVRDIYGVEI
                     VPGLEPLVILAVVACIDQLAHD"
     gene            complement(2836..3087)
                     /locus_tag="VHR64_16955"
     CDS             complement(2836..3087)
                     /locus_tag="VHR64_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304579.1"
                     /translation="MPKEATYLTLFEPATYSIRVQGALDANWSGRLGGLRILVVRAGD
                     QAVTELSGQLADQAALVGVLTSLYDLGMPLLSVERLAAN"
     gene            complement(3191..3694)
                     /locus_tag="VHR64_16960"
     CDS             complement(3191..3694)
                     /locus_tag="VHR64_16960"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX3304580.1"
                     /translation="MATLFALLYPDLPTRDAALETVHGLEDAGYLHVIESALVTKDPS
                     GHVEYKGSDRSLRSDTTKGLALGALTGVIFAVPVAGIAVGTVTGLMIGRHQQHGDSRE
                     FPEFARDVEQALQPGGCAVLMLAESESPDRVIQVMAKHGGKLMSTELSADRLAEIQKR
                     IDQASSV"
     gene            complement(3808..>4894)
                     /locus_tag="VHR64_16965"
     CDS             complement(3808..>4894)
                     /locus_tag="VHR64_16965"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX3304581.1"
                     /translation="DTATIFGLLGLATSYRIQGNFDDAAFTANYAVELAAEHDLKSGL
                     ALGQLILGSIAYELNDLEGAITHFQAASACKRRTVETVGIEALEGLALAYQHQGKEQD
                     AANELEQLRQLILEVRSLELLPELHAFEAHLALAGGDRERAIHWLASSGTSLDLGSLS
                     AIEVPLLTRVKVLLTEASDASIDEAQRHIDAVLERAIAIRDVQRQVQGLNLRAIAHHA
                     QGRRADGNAALADALRLGGPLGMVRSYLDAGPRLVPLLRDAARANEVAAYAARVLSAF
                     GGMAAPQRIQPNRSRFQIFEMLTEREAQILLCLSRQLTNQEIADELYISPLTVKRHNS
                     NIYDKLGVANRRQALIKADEMGLLPMA"
BASE COUNT          878 a         1646 c         1521 g          849 t
ORIGIN      
        1 ccgaaagcct cggcgcgctg gcgatccacg tccggaacga gcgcgagctt cgacaggccc
       61 tcgagcaagc caagcaggcc gatcgcacga cgctgatctc gatcgcggtc ccgatcgacg
      121 tccaggtgcc gggattcgag agctggtggg atgtcccggt agccgaggtc acggaccagc
      181 catcggtcga agccgcccgc gacgcctacg aggaaaagcg caagaaacag cgcgtcttca
      241 tttaggttgg tcgccggtcg tagtggcggg gctccgtacc cgccctgaga caacgactgt
      301 cccgttgaca tgcaccgacc gtctcaccga aaaggattcg atgagcgggt tcatacatgc
      361 gactgtggcg cttggcaggt ccaggcgggt acggagcccc gccactacgg aacgcgcgaa
      421 atgggagggg gaacgtgcat gcgggctgat ttgacgtttg gcgtgatcgg gcttggccgg
      481 atgggtatgc tccatgcgac gaacctgcgc ggcggcattg ccggcgcgcg gctcaaggtc
      541 gcggctgtct cgccggagga tcgcgcgcga ctcgaggcgg aagggtccgc gccggctccg
      601 ctggtcgatg tcgacgacct gatcgacgac ccggagatcc aggccgtcgc ggtcgtttcc
      661 ccgacggcca gccatcgcga gcatatcgag cgctgcgccg cggcgggcaa ggccgtcttc
      721 accgagaaac ctgtcgcctc gaccctcgac gacacgctgg ccaccgccga cgcggtccgg
      781 aagagcggga tcccgttcca gatcggcttc cagcgccgct acgatccgag ctacgcccgt
      841 gcccgcgagc tgatcgagca gggcgcgatc ggctccgtcg agatgttccg cgggattagc
      901 tgtgatggca tcccgccggt cagctatctg cgcaccagcg ggggtctctt ccgcgatctc
      961 ggcgtccacg acatcgatgc cgcccggttc ctgaccggcg aggagatcac cgaggtcagc
     1021 gcgaccgggg cgatcctgat cgaaccggcg ctggccgact tcgacgacgt cgattacggc
     1081 atcgtcaccc tccgcttcgc aaacggcgcc ctcggcgtga tccagaactg ctggcgcgcg
     1141 ccgtacgggt acgacatccg cgccgaagtg cacggctcca aaggcaaggt gatcgccgat
     1201 ctcgacgcga aggagccgac cgcgctctac accgaccgcg ccttctcctt ccgccgccat
     1261 cacctcttcg tcgagcgctt cgccgatgcg tatcgcctcg agctccaggc cttcgtcgac
     1321 gcggtccacg ccgatactgc gccgacccca ggcatcgagg acggcgtcag ggcgttcgtg
     1381 gtcgccgacg ccgctacccg gagccggatc gagaaccggt ggatcggagt gagttgaggg
     1441 ttgagggtcg agggaatggt caagccgcag tgcggggctg cggcttgacc agccggtgtt
     1501 aaccgacctc cattattgag acgggggttt tcaacccccg gcgggatcag gttaggcgcc
     1561 gctggcggtg ccggcgaagc tcggaggcgg ggcctcctcc gtatcggtcc cggagggctg
     1621 cttgctcaac agccggtaga ggtagaaggc atacgccagg aggagcaccc caaatacgat
     1681 cagcacggct ccgatgatat tgacccgtgt gctggaattg tccgtgttcc cggtaagaaa
     1741 caagatgccg aggacaatgc ttaacccacc ggtaatcagc gatccaatcc tgatgccact
     1801 ctcttcgcgc gccaggaccg tcgccgcgag cccgatcaga ccatatccga tcagccccag
     1861 gccgaggatg aaccgcgagg cgtccgcatc caggtaatcg gacaccggct ccagtatcac
     1921 gatgacacca acggcgacgc ccacgccgga acggaagagc cggtacggcg cggccggatg
     1981 cgtgggattg cgaaagctgg cataggcctc gatcgcggcg gtgaccagca ggatggctcc
     2041 gatcaactgg cgggcaatgt cgcgcgcctt gtccggctgc accaatgcgt aaatcccgat
     2101 aagagccagc aggccgccct caatccccac gatgtaccac ggaatgccgc gacgccatgg
     2161 cgcgctctgc agcattgtcg acttcgcccg atcctccatc tcattcatct ccctcgatca
     2221 agctggtcga cggccgcacc gcgccggcca acggcatcag tcgtgggcta actggtcgat
     2281 gcaggcaacg accgcgagga tgaccagcgg ctcaaggccg ggcacgatct ccacgccgta
     2341 gatgtctcga acccggaacc acttcttcga gaccaccgcg accgtgaatc catcccgctt
     2401 gatctcgtat tcgtggttga cgacgtttcc gtcggcatcc agatccgggc cgtcggtgac
     2461 gttgatcgcg aatcgctggc gcaccggggt gtacagcgcc ttcttgacca tcgcgatgcg
     2521 ctggccgttc ggaccctcga tctccatcga gtccttgacc cgcatccact tttcctggat
     2581 cttggcgacc tcatgcccct cggaatcacg gatgacgagc gtactgcgca agcggagcat
     2641 tttgccgtcg accttgaagg cccggtttcc ttcttcatcc tcgatgacgt agtcgtcccc
     2701 gaacgagaac atccgctcct tcatcttgta ccgcttgtgc ccgggcgtca tcgccgtcgc
     2761 gattcccccg gcttcctctt catccttgtg acggaacagt cgcatcgtac ttgctccttc
     2821 tgatggcgag atgtgtcagt tcgcggcgag ccgctcgacg gacaagagcg gcatcccgag
     2881 gtcgtacagg ctggtcaaca cgccgaccag cgccgcctga tccgccagtt ggcccgagag
     2941 ctcagtcacc gcctgatcac ccgcccgcac caccagtatc cgcagtccac ccaatcgccc
     3001 ggaccagttc gcatccagag ctccctggac ccgaatggaa tacgtggctg gctcaaacag
     3061 ggtcaggtat gttgcctcct ttggcatggc ccgcttgccc tccgcgtatc ccggttcgag
     3121 gaaatcgaca gccccatccc ggacgggaaa ggcaccatcg tcgaccagca ggttcagagc
     3181 gcacgcgcga tcagacgctc gacgcctgat cgatccgctt ctgaatctcc gcaagccgat
     3241 ctgccgaaag ttcagtcgac atcagcttcc cgccgtgctt cgccatcacc tggatcaccc
     3301 gatcgggaga ttcggattca gcaagcatca gcacggcgca accccctggc tgcaacgcct
     3361 gctccacatc gcgcgcaaac tcggggaact ccctggaatc gccgtgttgt tgatggcgcc
     3421 cgatcatgag tccagtcacg gtcccgacgg cgataccggc gaccggtacc gcgaagatca
     3481 cgccggtcag cgcgccgagg gcgaggccct tggtcgtatc ggaccgcagc gaccgatccg
     3541 agcccttgta ctcgacgtga ccgctcgggt ctttcgtcac cagggcagat tcgatcacgt
     3601 gcaggtaccc ggcatcttcc agcccgtgaa cggtctcgag ggccgcatcc cgggtgggga
     3661 gatcggggta gagcaatgca aatagcgtcg ccatctcgat cctccgtgcc gctatcctga
     3721 gactgccggt cgcggggtac atcgcccacg gggaaccaga aagggtgcag gcagattgag
     3781 cgcgcccacg cagggtgagc gcgcgcttta tgccatcggc aggaggccca tttcgtcggc
     3841 cttgatcagt gcctggcggc ggttcgcgac acccagcttg tcgtagatat tgctgttgtg
     3901 ccgcttcacg gtcaggggcg agatgtagag ctcgtcggcg atctcctggt tggtcagctg
     3961 ccggctcagg cagagcagga tctgcgcctc gcgctcggtg agcatctcga agatctggaa
     4021 gcgcgaccgg tttggctgga tgcgctgtgg ggccgccatg ccaccgaagg ccgacaggac
     4081 gcgcgccgcg tacgccgcca cttcgttggc ccgcgcggca tcgcgcagca ggggcaccaa
     4141 ccgcggcccg gcatcgagat agctccggac catgccaagg ggaccgccga gccgaagcgc
     4201 gtccgcaaga gcagcattcc cgtccgctcg tcgcccttgg gcatggtggg cgatggcgcg
     4261 caggttcaga ccctgcacct gccgttgcac atcccggatc gcgattgccc gctccaaaac
     4321 ggcatcgata tggcgttgcg cctcatcaat gctcgcgtcg cttgcctcgg tcagcaggac
     4381 cttgacccgc gtcagcagcg gcacttcgat cgcactcagg ctacccaggt cgagcgacgt
     4441 cccggagctg gcgagccaat gaatcgcgcg ctcgcgatca ccgccggcca acgccagatg
     4501 cgcctcgaac gcatgcagct cgggcagaag ctcgagactc cggacctcca ggatgagctg
     4561 ccggagctgc tcgagctcgt tggcggcatc ctgttccttg ccctggtgct ggtaggccag
     4621 cgccaggcct tccagcgcct cgatgcccac cgtctcgacc gtccgccgct tgcaggcact
     4681 cgcggcctgg aagtgcgtga tcgcgccctc gagatcattc agctcatagg caatgctgcc
     4741 caggatcaac tgcccgaggg ccagtccgga tttcagatca tgctcggccg cgagctcgac
     4801 cgcataattg gcggtgaacg ccgcatcatc gaaattgccc tgaatccggt atgacgttgc
     4861 gagcccaagc agcccaaaga tggtggccgt gtcg
//
LOCUS       DATAOZ010000675         2186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_523699, whole genome shotgun sequence.
ACCESSION   DATAOZ010000675 DATAOZ010000000
VERSION     DATAOZ010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523699"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VHR64_16970"
     CDS             <1..681
                     /locus_tag="VHR64_16970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3304582.1"
                     /translation="WEQISFVLPYTIELTLVAILIGLVLGVPIGTITAVRRNQPTDYF
                     GRVFSLIGLSLPAFYLGVLLMYFFSVRVDLFPAVGEPGWDDPIRNLRYLVLPALTLGL
                     IETAYIARMTRSVMITVLSDDYVRTARAKGLAEWSVLLRHALRAALVPIVSLVGIFAI
                     SLIGSSVLVEEVFARPGLGKLMVGATKQKDYTLLQSIMVVYALIIVLINLVTDIIYTV
                     VDPRVRYS"
     gene            681..1598
                     /locus_tag="VHR64_16975"
     CDS             681..1598
                     /locus_tag="VHR64_16975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3304583.1"
                     /translation="MTARGAALLNPGEEAEFRPGLPEPKLVSQRRRQLRSFAKNRPAV
                     VGLALILLIIAIALAAPLIAPHDPLDQSVRNRLAPPSNDYPLGRDDKGRDILSRVIFG
                     TRIALKVGIFSVLLGGALGTAIGVVAGYFGGKIDTALMRLTDVLLAFPDLITGLLVLA
                     VLGPGLWKMVLAIGLTITPRFARIAYGPTLSIKGTEFVDAARSVGVGHGRILGRHILP
                     NVGGDLMVFGSLWTASAIRLEASLSFIGLGVPPPTPTWGQMIREGTIHLTAVPLYSLG
                     PGIALLITVFAFNLVGDGLRDALDPRAQT"
     gene            complement(1667..1888)
                     /locus_tag="VHR64_16980"
     CDS             complement(1667..1888)
                     /locus_tag="VHR64_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304584.1"
                     /translation="MRATKTIWNLRNIVSGALIALALATGCLIGGVETASAQPPTLRE
                     ERGNSPYVVEAATQDPDPAYHHLGIGFLP"
BASE COUNT          330 a          637 c          788 g          431 t
ORIGIN      
        1 tgggagcaga tcagctttgt cctgccctac acgatcgagc tcaccctggt cgcaatcctg
       61 atcggactcg tcctcggcgt gccgatcggg acgataacgg cggtgcgccg gaaccagccg
      121 accgactact tcggacgggt cttctccctg atcggcctgt cgctgccggc gttctacctc
      181 ggcgtgctct tgatgtactt cttctcggtc cgggtggatt tgttcccggc ggtcggcgag
      241 ccgggatggg acgacccgat ccggaacctg cgctatctcg tactgccggc tctgacgctg
      301 gggctgatcg agacggccta catcgcgcgg atgacccgct cggtgatgat caccgtgctg
      361 tccgacgact acgtgcggac ggcgcgggcg aaagggctgg ccgagtggtc ggtcctgctg
      421 cgccatgcct tgcgggcggc gctggtgccg atcgtatcgc tggtcgggat cttcgcgatc
      481 agcctgatcg gcagctcggt gctggtcgag gaggtctttg cgcggccggg gctgggcaag
      541 ctgatggtcg gcgcgaccaa gcaaaaggac tacacgttgc tgcaatcgat catggtcgtc
      601 tacgcgctga tcatcgtgct gatcaacctg gtgacggaca tcatctatac cgtggtagat
      661 ccgcgggtga ggtacagcta atgaccgcgc gtggagccgc actgctcaat ccgggagagg
      721 aagcggagtt ccggccgggt ctgccggagc cgaagctcgt ctcgcagcgg cggcggcaac
      781 tgcggtcatt tgcgaagaac aggccggcgg tcgtcgggtt ggcgctgatc ctgctgatca
      841 tcgcgatcgc gctggcggcg ccgctgattg cgccgcacga tccactcgac cagagcgtgc
      901 gcaaccggct ggcaccgccc agtaacgact atccgctcgg tcgcgacgac aagggccggg
      961 acatcctctc gcgggtcatc ttcgggacgc ggatcgccct caaggtcggc atcttctcgg
     1021 tgctgctggg cggagcgctg ggcacggcga tcggcgtcgt ggccgggtac ttcggtggca
     1081 agatcgacac cgctctgatg cggctgaccg acgtgctgct cgccttcccg gacctgatca
     1141 cgggtttgct ggtgctggcg gtgctggggc cggggctctg gaagatggtg ctggcgatcg
     1201 ggctgacgat cacgccgcgc tttgcccgga tcgcgtatgg gccgacgctc tcgatcaagg
     1261 ggaccgagtt cgtcgacgcg gcacggtcgg tcggggtcgg gcatggccga atcctggggc
     1321 ggcacattct gccgaatgtg ggcggggact tgatggtgtt cgggagtctg tggacggcgt
     1381 cggccatccg gctggaggcg agcctgagct tcatcggctt gggcgtgccg ccgccgacgc
     1441 cgacatgggg ccagatgatc cgggaaggga cgattcacct gacggcggtg ccgctgtact
     1501 cgctcggtcc gggcattgcg ctgttgatta ccgtgttcgc gttcaacctg gtcggtgacg
     1561 gactgcggga tgcgttggat ccgcgggcgc agacgtgatg aaccgccggg cttcgcgaca
     1621 agcgccggcg gttcagcaga tgaatggtgg atgagcagtg ctcgtcctac ggaagaaagc
     1681 cgattccgag atggtgatag gcgggatccg gatcttgtgt cgccgcctcc accacatacg
     1741 gggaatttcc ccgctcctct ctgagcgtcg gcggttgggc tgaggcggtt tccacacctc
     1801 caatcaggca cccggtggcg agcgcgaggg cgatcaacgc tccgctgacg atatttcgca
     1861 ggttccagat ggtctttgtc gcgcgcatgt gttgctcctt ttgccgaggg ctaatccggc
     1921 gtcgcgagcc ggtcgttcgg ttcctcgggt atgggagcca tgctggactt cgggggcgcc
     1981 cggcacttcg actgaaggtc gtatcgggat gggaggagcg gaaatacgac gaacgtcgta
     2041 gtcgtcacgc cagtccgttg cggatggcga cggcggtggc ggcggagcgg gagtcgacgc
     2101 cgagcttggt gaagatggcg gtgacgtgga ccttgacggt ggcctccgca atgaagagct
     2161 gatcggcaat ggcccgattg gagtgt
//
LOCUS       DATAOZ010000676         9652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_1312679, whole genome shotgun sequence.
ACCESSION   DATAOZ010000676 DATAOZ010000000
VERSION     DATAOZ010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312679"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..1625
                     /locus_tag="VHR64_16985"
     CDS             426..1625
                     /locus_tag="VHR64_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX3304585.1"
                     /translation="MPRFTRRVALSLLAAPFLAPSVHLARAQDEEVASDTLLKVDLEA
                     KALPAPPAFMRLVRITLEPGATSPEHTHPGPEFGRIESGVVTITVKGPARIKQRSAKE
                     SDPFDEAEQNKENQLDRGDQIYYPAGTPMTFTNKGKEKASILALVVLPATNDHPPLID
                     YTAATPTADTFEGVTSRILGDAILTTAPSGPAMVTINDVALKPAQSLPGSRNPVLYSV
                     VKGDCSFKVTGGSVQISRTEKPGPQNDTELNKDIELKPGDAMFFPNGLRTTSRGENSG
                     SLELLQVLVEPASSDEKLSEENRGQLKFNQPSSPPEETPSSSGTAGNWRDGDSVYVNS
                     SDVNLRDSPSTNGGQVTVLIYGQELIIDGDPTDADGFTWWPVHIADNPDISGYVVQDF
                     IQSDPVQ"
     gene            complement(2009..3121)
                     /gene="mltG"
                     /locus_tag="VHR64_16990"
     CDS             complement(2009..3121)
                     /gene="mltG"
                     /locus_tag="VHR64_16990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX3304586.1"
                     /translation="MIRFVVSSLKVAVIVISTLAVALGGFQAFDHYRSQAEESAGVGE
                     PVVFTIGKNDDASEVARNLHKRGLIRSEQVFELTVRYVDRDIKPATYTLTKGMNVSTI
                     VDLITAEKSKAVTKVKDYKITVVEGWRAEQIADELDKLKYPPGGEAFMRATEDYPHDS
                     YDFLKGTKKGTLEGFLYPATYEFTNETSPEELVTMMLNAFDQAFTPEMRDRAEAMNLS
                     IYDVVKIASLIEREAVVDDERPLIADVYLKRYNDGWNLEADPTVQYVIGKRDGKWWPV
                     PTAKDLEDTDSAYNLYKHSGLTPTPICSPRKLSMLAVLQPAQSPFMFFVARKDNSGRH
                     LFAATGEEQTANQELINSGADLSEYDTKYTEYLTSP"
     gene            3264..4211
                     /gene="miaA"
                     /locus_tag="VHR64_16995"
     CDS             3264..4211
                     /gene="miaA"
                     /locus_tag="VHR64_16995"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX3304587.1"
                     /translation="MPGPELPFVIAIFGPTGVGKTAAGIDLALLYNGEVVNADSRYLY
                     RHLEIGVAKPTHEERRGVRHHLIDVFEPTDFITIAQVQQLAYRAIDEITGRARLPIII
                     GGTPLYMNAIVDGWRIPEVAPDWALREELAQRIERDGLPTVVAELAAVDPVAAGRSGQ
                     NPRRVIRALEIHHVTGRPMSELEGKEPPPYRILRVALNRDRDELYRMLDQRVDDQIAA
                     GLLDEVRELLAKGMSGEEPAFSSIGYRQLLPVISGEQTLEQAAERIKHDTHRYVRHQM
                     TWLRKTSGLQWIDTGNEAWLTELETLVHGHLASTSTTES"
     gene            4301..5227
                     /locus_tag="VHR64_17000"
     CDS             4301..5227
                     /locus_tag="VHR64_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304588.1"
                     /translation="MNALIVDPMNTVEAVLSPVLTARGWKVDHARHVYEIFDLIIKHP
                     PDLIVVHGDGAGGEAAHICQRFKQNVLTAGIPIILVEDSPPPAWMIAGLPADAILQAP
                     WESEEFFHHVEVLLPGQSVTSSLDDLTNFPRRASVIDEMQRRVLARELFGVGVLNLRE
                     ADAYRQDFGRSGLDQFVVLVSVLLRRHAAASTPISVGHLDDGNFLVIGPNSAVQEVIA
                     QTTYEFDALVPAYYEMDTLFGADDSHDVGPSTWIALQGALCLVEPDGFENILQIGYRL
                     SDTLTAGSDAIHALSRDEIPDQTSALTGTIQA"
     gene            5335..6195
                     /locus_tag="VHR64_17005"
     CDS             5335..6195
                     /locus_tag="VHR64_17005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX3304589.1"
                     /translation="MASEYHDPSVLVSTDWVAKHLDDPKVRIVESDEDILLYDIGHIP
                     GAVKMDWHTDLQDRVRRDFIDRAEFEALMGSAGIANDTTVVFYGDRNNWYATYAFWLF
                     KYYGHKDCRIMNGGRAKWEAEGRPYDRNVPSYPQTTYTAQEPDQSIRAFRDDVMALLP
                     SVESGEASLVDVRSPQEYSGEVIHMMGYPQEGAQRGGHVKGARNIPWGKAANEDGTFK
                     SAEDLTKLYQSEGVTPDKDVIAYCRIGERSSHTWFVLTYLLGHKNVRNYDGSWTEWGS
                     MVNVPIEKNV"
     gene            6198..6614
                     /locus_tag="VHR64_17010"
     CDS             6198..6614
                     /locus_tag="VHR64_17010"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX3304590.1"
                     /translation="MDRSEYIEYLLDHYQNPRHKHRMEGADVQLGGGNPGCGDLITIY
                     LKVDENDRITEASFEGEGCTISQAGGSIIAEMLPGMSLSEIKELGTGTMIDEMGEDIV
                     KSRVRCATLALGTAQAAVDQLLKDRVRAELRQRTGE"
     gene            6620..7705
                     /locus_tag="VHR64_17015"
     CDS             6620..7705
                     /locus_tag="VHR64_17015"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX3304591.1"
                     /translation="MPARFPVIDDRPTPPWTRAYGIALIGCGGIVNYAHLPAYAQHGL
                     KVVGCYDLNRDAALSTATAHGIPRVYESVDELLIDPDVTIVDIAVHPAAQPDLAVRCI
                     EAGKHILCQKPLALNPIEARRIVDSASLMAAKAAVNQQMRWSGGIAAAKQIIERGFIG
                     ELTDLQINVSVKTPWHMWPWLRDSDQLDVMFHSIHYIDAIRFLAGDPELVTSRHTTFP
                     GQPERAETKTVTVLDYATRCQALIAVNHHDESGGVHAWFRVLGTEGVIEGDIGLLATY
                     PEGGVDTVRARRGDEPEGEWHEPILKTLWMPDAFIGPMASLINAIDTGGTPLTNVADN
                     LNTVRVVTAAYESMAQGRTIPLNTIPG"
     gene            7713..9044
                     /locus_tag="VHR64_17020"
     CDS             7713..9044
                     /locus_tag="VHR64_17020"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEX3304592.1"
                     /translation="MMGTPGPIAEIDLCRAWTRLPRSIPLTTTDGRSVDVIHLGSWTH
                     GFGPDFRDAIISIDNGPTEHGSIELHLRTRGWSDHRHHLDPRYNDVILHIVGQHDEVE
                     TRRADGKLVPTVVLDIREMGLPTHAVDWSLVGGAVCAQRLARDQPDLIRGIVGRLGDN
                     RITSRAARIESRLTETTPDAVLYAEILDALGYVSNREPMAAIVSRLPWTNLVVIAGQE
                     AEAFDRTLAALLGVGGFLPLSDAEIANTGLEPDRVHAMATIWAGIAWEWDVKPIMSTE
                     WTLARIRPTNHPIRRLVQAAALVAGTRFHLTANLLGPLRAATDPTPALIEIVDRAGVP
                     ALGNDRARAIATNVLVPFGFALASQSGDADLAESTARVWESLPGAESNERTRRAVRQI
                     TGDVGMKRLGARLQQGLIHLDQTLCAPRRCYECPIAAIVVRESAADGIALG"
     gene            9090..>9652
                     /locus_tag="VHR64_17025"
     CDS             9090..>9652
                     /locus_tag="VHR64_17025"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX3304593.1"
                     /translation="MPPVPIIDSHVHLWDPVKVEIPWLSNVPKINVKRDLANYAEATS
                     GLDVKGLVYLQVEVAPPYALLEARDLVNLARSDKRVMGIVPWAPLEYGDWVRYFLEEI
                     AAIGPEIKGIRRIVQDEPDPEFCLQPRYIEGTRALPDYGLSADLCCHFRQLGPTVELV
                     RQCPNTQFILDHIAKPNIRGGEMEPWA"
BASE COUNT         1746 a         3238 c         3009 g         1659 t
ORIGIN      
        1 gacctgggag ccggacctga tggtgcccat ggccgcggcc gcggtggggc gcccgggagt
       61 ggccggaccg ccggataggg gcgggggctg cgcgcagggc ggcccagcgc actggccgac
      121 cacggcaact ccgtcgccag caggcaaccc tcctggcgca gcgcgatgcg gtccggctcg
      181 ccccggcttc gtcgcgtcgc atgccacccc aatgggtgac ccatgttccc gaacaggggt
      241 tacccgtgaa gccggtcggc acaatggtaa ccccggggct ccgtcacgca ggtgaccaat
      301 catcgtgtgc gccgctttgg aatcgtctcg caggtagccc atatgcgaaa ctctacaggc
      361 cgacgccgga gcacggaggt ctgccgtcgt gcgtcgccac tcccgggggt tgtatgtctg
      421 ccgtcatgcc acgattcaca cgccgggttg ccctgtcatt gctggcagcc ccgttcctgg
      481 cgccgtcggt gcacctcgcg cgcgcccagg acgaagaagt cgcgtccgac acattgctca
      541 aggtcgatct ggaggccaag gccctgccgg cgccaccggc cttcatgcgg ctggttcgga
      601 tcacgctcga gccgggcgca accagccccg agcacacgca tcctggaccg gagttcgggc
      661 ggatcgagtc cggcgtggtg acgatcacgg tcaagggtcc ggccaggatc aagcagcgct
      721 ccgccaagga gagcgatccc ttcgatgagg ccgagcagaa caaggagaac cagctcgatc
      781 ggggcgacca gatctattac ccggccggca cgccgatgac cttcaccaac aagggcaagg
      841 agaaggcgtc gatcctggcc ctggtcgtcc tgccggcaac aaacgatcac ccgccgctga
      901 tcgattacac cgccgccacg ccgaccgccg acacgttcga gggtgtgacg tcccggatcc
      961 tcggcgacgc gatcctgact acggccccca gcggcccggc gatggttacg atcaacgatg
     1021 tcgcgctcaa gcctgcccag tcgcttcccg gcagccggaa tccggtgctc tactcggtcg
     1081 tgaaaggcga ttgctcgttc aaggtgaccg gcgggtcggt ccagatcagt cgcaccgaga
     1141 agccgggtcc gcaaaacgac accgagctga acaaggacat cgagctcaag ccgggcgacg
     1201 ccatgttctt cccgaacggc ctgcgcacga ccagccgggg cgaaaactcc ggcagtctcg
     1261 agctgctgca ggttctggtc gagccggcct cgtccgacga gaagctctcg gaagagaatc
     1321 gcggccagct caagttcaac cagccgagca gcccacccga ggagacgccg agcagctccg
     1381 gcacggccgg gaattggcga gacggcgatt cggtttacgt caactcgtcc gacgtcaacc
     1441 tgcgcgattc tccgagcacg aacggcggtc aggtcacggt cctgatctac ggccaggaat
     1501 tgatcataga cggcgacccg accgatgccg atggcttcac ctggtggccg gtccacattg
     1561 ccgacaaccc ggatatcagc ggctacgttg tccaggactt catccagtcc gatccggtgc
     1621 aatagcggtt ccaggaatcg ccccggactg aaggccgggg ctgagggact atgaggcggc
     1681 tgaagccgac tgactcagac gtagccccgg ccccctgtgg ccgtctgggg tgacggaatg
     1741 cgcgttattg gagccgatcc gcctccatca acctccacag ccgacaccga agcggtccag
     1801 aggcgggccg cgctacgcca ctaacgtcag ttcagtccct ttagtggact tttcgtgggt
     1861 cagcccgagc ctttagcctc gggcggctcg gaacgccgca ttcacccacg cgggaccggc
     1921 ctccgtccaa gcggggcatg gtagccccgc cctacgtttg ggttgcggtg cccgcaaacg
     1981 atcgtttcgt tagccacggg gcggcgccct aggggctggt cagatattcg gtgtacttgg
     2041 tgtcgtactc ggataggtcg gcgccgctgt tgatcagctc ctggtttgct gtttgctctt
     2101 cacccgtggc agcgaacagg tgccgccccg agttgtcctt gcgcgcgacg aagaacatga
     2161 acggcgattg agccggctgc agaaccgcca gcatcgagag cttgcgcgga ctacaaattg
     2221 gcgtcggcgt caatcctgag tgcttgtaga ggttgtaggc cgaatcggtg tcttccaggt
     2281 ccttcgcggt cggcactggc caccacttgc catcgcgctt gccgatcacg tactggaccg
     2341 tcgggtctgc ctccagattc cagccgtcgt tgtatcgctt caggtaaaca tcggcgatta
     2401 gcggtcgctc atcgtcgacg acggcctcgc gctcgattag cgatgcgatc ttgacgacgt
     2461 cgtagatcga gagattcatc gcctcggccc ggtcccgcat ctccggcgtg aaggcctggt
     2521 cgaaggcgtt gagcatcatc gtgaccaact cttccggcga tgtctcattg gtgaactcgt
     2581 aggtcgccgg gtagaggaat ccttccagcg tgcctttctt ggtgcccttc agaaagtcgt
     2641 aggagtcgtg cggatagtcc tcggtcgcgc gcatgaaggc ctcgccgccc ggcgggtatt
     2701 tgagcttgtc cagctcatcg gcgatctgct ctgcccgcca gccctcgacg acggtgatct
     2761 tataatcctt gaccttggtg accgccttgc tcttctcggc cgtgatcaga tcgacgatgg
     2821 tcgagacgtt catgcccttc gtcagcgtgt aggtggccgg tttgatgtcg cggtcgacgt
     2881 agcggacggt cagttcaaag acctgctccg aacgaatcag gccgcgcttg tgcaggttcc
     2941 tggcaacttc gctcgcgtca tcgttcttgc cgatcgtaaa gaccaccggc tcgccgacac
     3001 cggccgattc ctcggcctgg ctccggtaat gatcgaaggc ctggaaaccg ccgagcgcca
     3061 cagcgagcgt gctgatcacg atcaccgcga ccttcagact tgaaacgaca aaccggatca
     3121 tctatttcgt gctcccgaac ctagctcgga cgatgacacc ttcacattgg gagttcaggc
     3181 gggatactcg cggatgcggc gcatacttgt ccgcgcgcaa aagtgtaccg cccggatgcg
     3241 cgcgggacta gcagcgagcc gatatgcccg gacctgaact ccccttcgtg atcgccatat
     3301 tcggcccgac cggcgtcggc aagacggctg cgggcatcga cctggccctc ctatacaacg
     3361 gcgaggtcgt caacgcggat tcccgctatc tctaccgcca cctcgagatc ggtgtcgcta
     3421 aaccaacgca cgaggagcgc cgcggggttc gccatcacct gatcgatgtc ttcgagccga
     3481 ccgacttcat cacgatcgcc caggtccagc aactcgccta tagggccatc gacgagatca
     3541 ccgggcgtgc caggctcccg atcatcatcg gcggcactcc gctttatatg aacgcgatcg
     3601 tcgacggctg gcggattcca gaggtcgcgc cggactgggc attgcgggaa gagctggccc
     3661 agcgcatcga gcgggacggc ttgcccactg tcgtggccga gctggccgca gtcgatccgg
     3721 tggcagccgg gcgatcgggt cagaatccgc gccgggtcat ccgcgccctc gagatacacc
     3781 acgtgaccgg ccggccgatg tccgagctcg aaggcaagga gccgccaccg tatcgcatcc
     3841 tgcgggtcgc cctgaaccgg gaccgagacg agctctaccg catgctcgat cagcgggtcg
     3901 atgaccaaat cgccgccggg ctactcgatg aagtccgtga actgctcgca aaggggatgt
     3961 cgggcgagga gccggccttc tcctcgattg gctaccggca actgctgccc gtgatctccg
     4021 gcgagcagac acttgagcag gccgctgagc ggatcaagca cgatactcac cgctacgtcc
     4081 gccaccagat gacctggttg cggaaaacgt ccggcctgca atggatcgac actggcaacg
     4141 aagcgtggct gaccgagctg gaaacgctgg tgcacggcca cctcgcgtcc accagtacca
     4201 ctgaatcctg actttggggt gatacggacc atttcccgcg tcgccggcac cgcctacgat
     4261 gatgcgcgag gcgacgaatc tggaggattg cccgctgccc atgaatgcgt tgatcgtcga
     4321 tcccatgaac acggtcgagg ccgtgctgtc tcctgtcctg accgcacgcg gttggaaggt
     4381 cgaccacgcc cgccacgtct acgagatctt tgacctgatc atcaagcatc cgccggatct
     4441 gatcgttgtc catggcgacg gtgcgggcgg cgaggcggcg catatctgcc agcgcttcaa
     4501 gcagaacgtc ctgacggccg ggatcccgat catcctcgtc gaggattcgc cgccgccggc
     4561 ctggatgatc gccgggttgc cagccgatgc catcttgcag gccccgtggg aatctgagga
     4621 attcttccac cacgttgaag tcctgctgcc gggccagagc gtgaccagct cgctcgacga
     4681 cctgacgaat tttccgcgcc gggcatcggt gatcgacgag atgcagcgcc gcgtgctggc
     4741 gcgcgagctc ttcggcgtcg gcgtcctcaa cttgcgcgag gccgatgcct accggcaaga
     4801 cttcggacgg agtggcctcg atcagttcgt ggtgctcgtc agcgtactcc tgcgccgtca
     4861 tgcggccgcc tcgacgccaa tcagcgtcgg ccacctcgac gacggcaact tcctggtgat
     4921 cggcccgaac tccgcggtcc aggaagtcat tgcccagacc acctacgagt tcgacgcact
     4981 cgtgcccgcc tattacgaaa tggacaccct cttcggcgcg gacgactcgc atgatgtcgg
     5041 cccgtcgacc tggatcgcgc tccagggcgc gctctgcctg gtcgagccgg atggcttcga
     5101 gaacatcctg cagatcggct accggctctc cgacaccctg accgccggct ccgacgcgat
     5161 ccacgccctg tcgagggacg agatcccgga ccagacatcc gcgctgaccg gcacaattca
     5221 ggcctgatcc cggcccattc cgcctgaata cgctcgtata ctggtgtcac gaccggcgcg
     5281 cccgcttggg cccggtcaac ttctcgcatt cctcagacac tggagtccca aaatatggcg
     5341 agcgaatatc acgatccgtc ggtgctggta tcgacggact gggttgcaaa gcacctggat
     5401 gatccgaagg tccgcatcgt cgagtccgac gaggacattc tgctttacga catcggccac
     5461 atcccgggcg cggtcaagat ggactggcac accgatctgc aggaccgggt gcgccgcgac
     5521 ttcatcgaca gggccgagtt cgaggcgctg atgggctcgg ccggtatcgc caacgacacg
     5581 acggtcgtct tttacggcga ccgcaacaac tggtacgcca cgtacgcctt ctggctcttc
     5641 aagtactacg gccacaagga ttgccggatc atgaacggcg gtcgggccaa gtgggaagcg
     5701 gagggccggc catacgaccg caacgttccc tcctacccgc aaacgaccta caccgcccag
     5761 gaacccgacc agtcgatccg cgccttccgc gacgacgtga tggccctgct gccgagtgtc
     5821 gagtcggggg aggcgtcgct ggtcgacgtc cgctctccgc aggagtactc cggcgaggtg
     5881 atccacatga tgggctatcc gcaggagggt gcccagcgcg gcggtcatgt caagggtgcg
     5941 cgcaatatcc cgtggggcaa ggccgcgaat gaggacggca ccttcaagag tgccgaggac
     6001 ttgacgaagc tctaccagtc ggaaggcgtc acgccggaca aggatgtgat cgcatactgc
     6061 cggattggcg agcgctcgag ccacacctgg ttcgtgctga cctacttgct cggccacaag
     6121 aacgtccgca actatgacgg ctcgtggacc gaatggggca gcatggtcaa cgtcccgatc
     6181 gagaagaacg tctgactatg gatcgatccg aatacatcga gtacctgctc gaccactacc
     6241 agaatccgcg ccacaagcac cgcatggagg gcgctgacgt ccagctcggc ggcggcaacc
     6301 cgggctgtgg cgacctgatc acgatctacc tcaaggtcga cgagaacgac cgcatcactg
     6361 aggccagctt cgaaggagaa ggctgcacca tcagccaggc cggtggctcg atcatcgccg
     6421 agatgctgcc cggcatgtcg ctctccgaga tcaaggagct cggcaccggc acgatgatcg
     6481 acgagatggg cgaggacatc gtcaagagcc gcgtccgctg cgcgacgctc gctctcggca
     6541 ccgcacaggc ggccgtcgat cagcttctga aagacagggt gcgggccgaa ctacgccagc
     6601 gcaccggcga atagcaccga tgccggcccg ctttcctgtc atcgacgacc gcccgactcc
     6661 gccctggacg cgggcatatg gcatcgccct gattggctgc ggcggcatcg tcaactacgc
     6721 gcatctgccg gcatacgccc agcacggcct caaggtggtc ggctgctacg acctcaaccg
     6781 tgatgcggcc ctctcgaccg caaccgctca tggcatcccg cgcgtttacg agtcggtcga
     6841 cgagctgctg atcgatcccg acgtcacgat cgtcgacatc gccgtccatc ccgccgcgca
     6901 gccggatctg gcggtacgct gcatcgaggc cggcaagcac atcctctgcc agaaaccgct
     6961 cgcactcaat ccgatcgagg ccaggcgaat cgtcgattcg gcctcgctga tggcggcaaa
     7021 ggcggccgtc aaccagcaga tgcgctggtc gggcggaatc gcggcagcga agcagatcat
     7081 cgagcgcggc ttcatcggcg aactgaccga tctccagatc aacgtttcgg tcaagacgcc
     7141 ctggcacatg tggccatggc tgcgagattc cgatcagctc gacgtgatgt tccactcgat
     7201 ccactacatc gacgcgattc gcttcctggc cggcgatccg gagctggtta cgagccggca
     7261 caccacattc ccgggtcaac ccgagcgagc cgagaccaag accgtaaccg tgctcgatta
     7321 cgcaacgcgc tgccaggcat tgatcgcggt caatcaccac gacgagtcgg gcggcgttca
     7381 cgcctggttc cgcgtcctcg gcacggaggg cgtgatcgaa ggcgacatcg gcctgctcgc
     7441 gacctacccg gagggcggcg tcgacacggt ccgggccagg cggggcgatg agccggaggg
     7501 cgagtggcac gagccgatcc tgaagacgct ctggatgccg gatgccttca tcgggccgat
     7561 ggcctcgctg atcaatgcga tcgacacggg cggcacgccg ctcacaaacg tcgccgacaa
     7621 cctgaacacg gtccgtgtcg tcaccgccgc ctacgaatcg atggcgcagg gccggacgat
     7681 cccgctcaac acgataccgg gttgagctag cgatgatggg gacgcccggg ccgatcgctg
     7741 aaatcgacct ctgtcgcgcc tggacgcgcc tccctcgctc gatcccgctg acgaccaccg
     7801 acggccgctc ggtcgacgtc atccacctcg ggagctggac gcacggcttc ggcccggact
     7861 tccgcgatgc gatcatctcg atcgacaacg gcccgaccga gcatggcagc atcgagctgc
     7921 acctgcggac gcgaggctgg tccgatcacc gccatcacct cgatccccgc tacaacgacg
     7981 tgatcctgca catcgtcggc cagcatgacg aggtcgagac gcgcagagcg gacggcaagc
     8041 tcgtgccgac cgtcgtgctc gatatccggg agatggggct gccgacgcac gcggtcgact
     8101 ggtcgcttgt cggcggcgcg gtctgcgccc aacggctggc gcgtgatcag cccgacctga
     8161 ttcgcggcat cgtcgggcgc ctcggcgata atcgcatcac atcccgcgcg gcccggatcg
     8221 agtcgcgcct gaccgagacg acgccagacg cagtcctcta tgccgagatc ctcgatgccc
     8281 taggctacgt ctcaaaccgc gagccgatgg ccgctatcgt ctcgcgtctg ccgtggacga
     8341 acctcgtggt aattgcgggt caggaggcgg aagcgttcga tcgaactctt gccgccctgc
     8401 tcggcgtcgg aggattcctg ccgctttccg atgccgagat cgcaaacacc ggactggagc
     8461 ccgaccgcgt gcacgctatg gcgacgatct gggccggtat tgcctgggag tgggacgtca
     8521 agccgattat gtcgacggaa tggacgctgg cccggattcg gcccaccaat cacccgattc
     8581 gccgtctggt ccaggctgcc gcccttgttg ccggcaccag gtttcatctc acggcgaacc
     8641 ttctcggtcc gctgcgcgct gcgaccgacc cgaccccggc attgatcgag atcgtcgacc
     8701 gggccggtgt tcccgcgctc ggcaacgacc gtgcccgggc gattgccacc aacgtgctgg
     8761 tccccttcgg ctttgcgctc gccagccaaa gcggtgatgc cgatctggcc gagagcacgg
     8821 cccgcgtctg ggagtcgcta ccgggcgcgg aatccaacga gcgcacccgc cgcgcagtcc
     8881 gccagatcac cggcgacgtc ggcatgaagc ggctcggcgc tcgtctccag caaggactga
     8941 tccacctcga ccagaccctc tgcgcgccgc gccgctgcta cgaatgcccg atcgccgcga
     9001 tcgtggtccg ggaatcagcg gcggacggca tcgcgctcgg gtaaagtgtc gcggaggctt
     9061 ccgctgctgc gtccggagtg aattgcgcca tgccgccagt cccgatcatc gactcgcacg
     9121 tccacctctg ggatcccgtc aaggtcgaga ttccgtggct ctcgaacgtg ccgaagatca
     9181 acgtcaagcg tgacctcgcc aactacgccg aggcgacgtc cgggctcgac gtgaaggggc
     9241 tggtctatct ccaggtcgag gtcgcgccgc cctacgccct gctcgaggcg cgcgatctgg
     9301 tcaacctcgc ccgctcggac aagcgcgtga tgggcatcgt gccgtgggcg ccgctcgagt
     9361 acggcgactg ggtccgctac ttcctcgagg agatcgcggc gatcgggccg gagatcaagg
     9421 gcatccgccg gatcgtccag gatgagccgg atccggagtt ctgccttcag ccgcgctata
     9481 tcgaagggac acgcgcgctg cccgattacg gcctgagcgc cgatctctgc tgccacttcc
     9541 gccagctcgg cccgacggtc gagctggtcc ggcaatgccc gaacacgcaa ttcatcctcg
     9601 accacatcgc caagccgaac atccgcggcg gcgagatgga gccgtgggcc ag
//
LOCUS       DATAOZ010000677         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4207019, whole genome shotgun sequence.
ACCESSION   DATAOZ010000677 DATAOZ010000000
VERSION     DATAOZ010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4207019"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VHR64_17030"
     CDS             <1..1121
                     /locus_tag="VHR64_17030"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX3304594.1"
                     /translation="FPFEGGLSGESARMFCDILLPDGSPSWADPRHVLRRSLSRAAEK
                     GFTFYTHPEIEFFLVEDFPPDGGAPTPVDSGGYFEHTTHAIARDFRRQAVLALERIGI
                     SVEFSHHEVAPGQQEIDLRYADALTTADNVMTFRHVVKEVGLSHGVRATFMPKPFTDQ
                     PGSGMHTHLSLFEGERNAFHDPSDPMKLSKVARSFIAGLLTHAREYAAITNQWVNSYK
                     RLFPQSIEGRITESPAYVCWGHLNRSALVRVPAYGKPNSARVEVRSLDSACNPYLTYA
                     VLLGAGLKGIEEGYELPPGAEDDVWSLSDAERRAMGYETLPGNLSEAIDVMSRSEFVA
                     EVLGEHVFDFFLRNKRAEWDEYRRVVTPYERQRYLGML"
     gene            complement(1274..2470)
                     /gene="mycP"
                     /locus_tag="VHR64_17035"
     CDS             complement(1274..2470)
                     /gene="mycP"
                     /locus_tag="VHR64_17035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated serine protease
                     mycosin"
                     /protein_id="HEX3304595.1"
                     /translation="MTANTVVRRSTDRWLATNTVRITAVALVCYLLSALTAAAAAGDS
                     IRNQEWIVDSLNLSETHHYSEGKGVTVAVVDTGVDAKHIDLSGNVLAGSDFTTGSPRG
                     NGTRDMAGHGTEMAGLIAGHGHGRGHESGILGIAPSATILPIRVSASDLGNGASDAAA
                     VTWAATHGATVICMAFGGANSNAEIAAAMLEAYQANVVLVAAAGNVNQGDRIVQYPAA
                     YPGVVAVAGTDEHGNHSKVSVTGNLITIAAPSDNIPSTYNNGGYAIGSGTSSSTAIVA
                     GAAALVRSRFPNLTAGEVVNRLTATAIDKGPPGRDDEYGYGLINLVGALTAKVPPAGS
                     ATRTPPAAAAAPSTNRPTPSERHSTSLAPASRSTSTTIISIVGVVIVLFTVLGAWTLV
                     RRRRPG"
     gene            3307..3714
                     /locus_tag="VHR64_17040"
     CDS             3307..3714
                     /locus_tag="VHR64_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304596.1"
                     /translation="MFGDGGVGLAHNRMRLVVERLRPVVGRHHVTDSCLVLFPRLVQR
                     VHLGGGAVDGRHDSGQCAVCVDSAFGRGAHRLSHRIDLSEERSGCAAGGCGPRLGQRR
                     AGGLEPLPLVGQRLVRLLPRHRVRAGIGRLLGQ"
BASE COUNT          626 a         1237 c         1264 g          747 t
ORIGIN      
        1 tcttcccgtt cgagggtggc ctgtccggcg agagcgcccg gatgttctgc gacatcctgc
       61 tgcccgacgg gtcgccgagc tgggccgacc cgcggcatgt tctgcggcga tcgctgtcgc
      121 gcgcggcaga gaagggcttc acgttctaca cgcaccccga gatcgagttc ttcctcgtcg
      181 aggatttccc gcccgacggt ggcgcgccca cgccggtgga ctccggcggt tatttcgagc
      241 acacgacgca cgcgattgcg cgtgattttc ggcgtcaggc cgtcctcgcg ctcgaacgga
      301 tcgggatctc ggtcgagttc agccaccacg aggtcgcgcc cggccagcag gagatcgacc
      361 tgcgctacgc cgatgcgctg accacggccg acaacgtgat gacgttccgc cacgtggtca
      421 aggaggtcgg cctctcgcac ggcgtacggg cgacgttcat gccgaaaccc ttcaccgacc
      481 agcccggcag tgggatgcac acgcacctgt ccctcttcga gggggagcgc aacgcgttcc
      541 acgatccgtc cgacccgatg aagctgtcga aggtcgcgcg ctccttcatc gccgggctgc
      601 tgacccacgc ccgggagtac gcggcgatca caaaccaatg ggtgaactcg tacaagcggc
      661 tgttcccgca gtcgatcgag ggtcggatca ccgaatcgcc ggcctacgtc tgctggggtc
      721 acctgaaccg ctcggcgctc gtgcgcgtcc cggcctacgg caagccgaac tcggcccgcg
      781 tcgaggtgcg atcgctcgat tccgcctgca atccatactt gacctacgcg gtgctgctcg
      841 gcgccggcct caagggcata gaagaggggt acgagttgcc gcccggcgcc gaggacgacg
      901 tgtggtcgct atccgacgcg gaacgccggg cgatgggcta tgaaacgttg cccggcaacc
      961 tgtccgaggc gatcgacgtg atgagccggt ccgagtttgt cgccgaggtc ttgggcgagc
     1021 acgtcttcga ctttttcctg cgcaacaagc gcgccgagtg ggacgagtac cggcgcgtgg
     1081 tcaccccgta cgagcgccag cggtatctcg gaatgctctg acgttcggtg caagtggcgt
     1141 cgacgcactc tgttgccgcc gttgtggctg cgtgccttcg tgaagtagac ggcgggtatc
     1201 ttgtgcactc gggcggtttc gagcagtggc cgcgtacggt caatcgccgc ccagccgtgt
     1261 gggactcggt cacttaccca ggccgccgtc gtcgtacgag cgtccacgcc ccgaggacgg
     1321 tgaacagcac gatcacgacg cccacgatgg agatgatcgt ggtactcgtg ctgcgcgacg
     1381 ccggggcgag cgaggtggaa tggcgctcgg acggagtcgg tctgttcgtc gacggtgccg
     1441 ccgccgcggc cggtggggtc cgcgtcgccg atccggccgg cggcactttg gccgtgaggg
     1501 cgccgacgag gttgatgagg ccgtagccgt actcgtcgtc gcgtcccggc ggtcccttgt
     1561 cgattgccgt tgccgtaagg cggttgacga cttcgcctgc cgtcagattc ggaaatcgag
     1621 accgtacgag cgccgccgct ccagcgacga tcgccgttga tgaactcgtt ccgctcccga
     1681 tggcgtagcc accattgttg tacgtcgacg gaatgttgtc ggatggcgca gcgatggtga
     1741 tgagattgcc ggtcaccgac actttggaat gattgccgtg ttcgtcggtg cccgctacgg
     1801 caacgactcc cgggtacgcc gccggatatt gcacgattcg gtcgccttga ttgacgttac
     1861 cggctgccgc gaccaagacg acgttggctt gatatgcctc aagcatcgcg gcggcgatct
     1921 ctgcgttcga gttggctccc ccaaacgcca tgcagataac agtggcgcca tgtgtggccg
     1981 cccatgtaac tgcagccgcg tcggatgccc cattccccaa gtcgctggcc gacactcgta
     2041 tcggaaggat cgtggccgat ggtgcaatcc cgaggatgcc actctcgtgg ccacggccat
     2101 ggccgtggcc agcaatgagt ccggccattt cggtgccgtg tccggccatg tcccttgtgc
     2161 cgtttccacg cggagaaccc gtcgtgaaat cggaaccggc taagacgttt ccgctcaaat
     2221 ctatgtgctt ggcatcgacg ccagtatcga ccaccgcgac cgtaactcct tttccctccg
     2281 agtagtgatg tgtctcggag aggttcagcg agtcaacaat ccattcctga ttgcgaattg
     2341 agtcaccggc tgctgcggca gcagttagtg cagacaagag gtagcagaca agtgcgacgg
     2401 ctgttatgcg aactgtgttc gtcgctaacc accggtctgt cgatcttctc acgacggtgt
     2461 tggcagtcac cgatcgatac ctatgacgcc tggtcctgga tcgtgcagta tcggagcctc
     2521 ggccacaggc tcaagaatgg acgacacacc gacgggcatg ggccaatcga cgtgtgtatc
     2581 ccgacgtccc gatcgactgc ttccggtaga agtcgtcgat gcgccgccca tcatgccacc
     2641 tatgggtaag gcgtcggtgc ttgtggtaga tcccctgaaa agcgacgatg gggaaccttc
     2701 ttgaccggat ctgacggcat tcccatcgga gtgcacggtc aggtctggct tctgcgcatt
     2761 aggtcgcact gagtcgtccg ggcgacccga gatgggcgta ttgccagcag atccccgacg
     2821 tgggattgcg tgccctttcg gattgagtcg gtcgctcgcg cgaggaagcg cggacacatc
     2881 gcctatcgcc ggcgacaggt catttgtggg ttgaagcccg ccgacttgac cttgcctccc
     2941 gtctcgcgat ccattggatt ggtcggaact cactccggac tcagacccgg tggtcaggcc
     3001 gctcgtagga ttccgtgcac cgctccccgc cgcagtgagg gttgtggctc caacctgccg
     3061 atgcgcaacg gtggaaccag cgacctgtgg acttgtttgt acactttgag cggaaacgct
     3121 cccgatatcc gagctaccgc caacggcgga tagcagcggg ctacctgatg tcaacgacga
     3181 agcggcgccc ggtcgtgtcg atgaggacac gtgcccgccg gtactactgt gcgtcaacgg
     3241 cagcggcgtc gaagtctgat gactcggctg cggcgcgtac ggaacgggtg gggcgagctg
     3301 ggtcgtgtgt tcggcgatgg cggcgtcggc ctggcccata accgtatgcg cctggtcgtt
     3361 gagcggctgc gtccagtcgt cggtcggcac cacgtcaccg atagttgcct ggttctgttt
     3421 ccacgattgg tgcagcgcgt ccaccttggc ggtggcgccg tcgacggccg tcacgattcc
     3481 ggtcagtgcg ccgtgtgcgt tgacagcgct ttcggccgtg gcgcccatcg cctgagtcac
     3541 cgcatcgacc tcagcgaaga acgatccggc tgcgccgccg ggggttgcgg gccacgcctg
     3601 ggtcaacgcc gcgcgggcgg cctcgagccg ctgccgctgg tcggtcagcg tctcgtacgc
     3661 ctgctgccac gccatcgcgt gcgtgccgga atcggtcggc tgctcggcca gtagtccggc
     3721 caacccgggc acgtcgtacg gcttccagtc ggcggcgctc acgaatactg agcctgtccg
     3781 ggcacctggg gcaacggcgt atcgcggccg acaatcgtgc caatcgtcat ctcgatgtcc
     3841 gcgaccgagg ctccacgcaa cgcgtcgacg gtcc
//
LOCUS       DATAOZ010000678         6257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_7099747, whole genome shotgun sequence.
ACCESSION   DATAOZ010000678 DATAOZ010000000
VERSION     DATAOZ010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7099747"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VHR64_17045"
     CDS             <1..521
                     /locus_tag="VHR64_17045"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX3304597.1"
                     /translation="DELRREAAEGADLMITIGGASLGDRDVLTELASDMVALDCWNIA
                     MKPGKPLVYGQIDGAVVIGLPGNPAAAFISAVQFVRPVIATMLGRLDIDPPTVVARAT
                     ESVTNSGGRRNYLRVTLEVEESGYVARLAGPQNVASLATLSRAEGLLIVPEGIERVEA
                     GERFDVQIIRTL"
     gene            628..2286
                     /locus_tag="VHR64_17050"
     CDS             628..2286
                     /locus_tag="VHR64_17050"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX3304598.1"
                     /translation="MTNRPVLPSVSRRTLLQASAVGVAAVPVLAYAQNSTPIASPVSS
                     PVAPPPAGSPAASPAASPTPEVTVIEWPTYGQDLTGDKAIPDGVITSQNVAGLTELWT
                     VDVGGPISATPVISGGIAYIGSYDGTLYAINIASGNVIWTYDTGAAVEEPNLKINLGV
                     TGSAHVENGVVYVGDAAAMVHAIDTATGQAIWTRKVDDQEHASIWSSPVVWNGTVYVG
                     VASVAKQIGFRGSVVALNAESGEQVWKTFMVPEDADGAGVFAVPAIDEQRGMLFVGTQ
                     NAYSESDAPYGNPISIVALNASSGDIAWTYNAPPNDGNKAPTDDVGFSASPNLFTATI
                     DGVDRDLVGEGQKSGDFIVLDRETGEKVWTATVSPAGFLGGMEGTSAVFNGIIAVPAT
                     DWQDPSGQGTGMVTGLDATTGEQRWTAQQDAPAPAPVAISNDVVFHGGFDGVLHAYSL
                     TDGTELFTADLGASVSGGTAVADGVVIVGAATPQFADFIKTGNQIHAFGFIAEATPAA
                     SPTTEEQVASPTAVPPTEAPVTSPTAEAPTETPSPTEEASPVIA"
     gene            complement(2350..2952)
                     /locus_tag="VHR64_17055"
     CDS             complement(2350..2952)
                     /locus_tag="VHR64_17055"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX3304599.1"
                     /translation="MIDRSELEPDLDEYGRVGYGKLARYSPIVLAAIMVAAIVFIAIN
                     QRDKKTTTREIIGQPAPNLTFTGFDGGTPTDLASMRGDVVVLNFWASWCEPCRTEMPA
                     FEAVHQSAGGDVRIIGVNIKNDRVENATELLAETGVTYQIARDDGGANPLYGPIETAL
                     GTGGSYPVTVVIRPDGVIDKVTVGPMDEAQIRDAIEKARS"
     gene            complement(2949..3434)
                     /locus_tag="VHR64_17060"
     CDS             complement(2949..3434)
                     /locus_tag="VHR64_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304600.1"
                     /translation="MGRRAQSIISTVLLAISAALLVAVVVLYVRDRNQPDEPKPPTPV
                     PGHNQAIDVLDAFRTQNLSAEFGEQGTDVRSTMLERPGQMIKVGSARAYVFIYPDVGS
                     QEQATLDVQPEDVDLVDINGDDVQYTSIDLYAGSNVAVVLVDADQQTADRVKAAVEGL
                     T"
     gene            3550..4512
                     /locus_tag="VHR64_17065"
     CDS             3550..4512
                     /locus_tag="VHR64_17065"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304601.1"
                     /translation="MDRREFLMGFPLLVAGTPEAESGSWVRRADMPVARSETPAAVLD
                     GKIYVAGGFGAGARADRYDPATDQWQQLADLPVETNHPGIAVLDGHVVVAGGYAMDGA
                     SAHNGMWAYHPGADSWEVIGELPEAMGAFGLATVDGDLYLVGGAFGSLNGEPSAAVWR
                     WRPQDGTWETRAPLAQAREHLAVVAVAGKIYAVGGRAHGRDNDQLGGAVERYDPAADK
                     WDSLASLPHPRSGLNGAKVCETVVVVGGETSKRVFDDVQILSLDGASWASLPDLPLAV
                     HGVAVASVNNRLFAIGGSTLAGRIQNVTAVYQLDLGTTRGACAS"
     gene            4773..5144
                     /locus_tag="VHR64_17070"
     CDS             4773..5144
                     /locus_tag="VHR64_17070"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX3304602.1"
                     /translation="MRQLSALQFIDGTETTLGDVARNLNVTPAVVTGLIDRLERRGYV
                     RRVASVFDRRRVYIELTTAGEDVRDHAEDQLAKRLDDLLSKLSDDEVTALEDGLRVME
                     KVMASVEPRRNKPGIKSEFGS"
     gene            complement(5122..>6257)
                     /locus_tag="VHR64_17075"
     CDS             complement(5122..>6257)
                     /locus_tag="VHR64_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304603.1"
                     /translation="NLDPLAAPRSYLTEINRMLWNRGPDRPPIGYLELLVDFAVNLGL
                     IQEPEDGSTQFERTSAMRDWRTKSWADQTARIRSIWMSSPVWIEGQGRQDVEPWNVDW
                     RGFRVKLLSHLAALEKDKWYRVADVAQWISEYDPAIVGPDATVAVSHATAQPGQSPHQ
                     ESVAYLAAAVIQSILVWLGFVRLHEPGRNERLMLVTDELRRVTRAEVEDRAASRAAPS
                     IAVADDLTIHLTDPEPIHVWSVLAFADALSLGRESVFRITPDSLRTAQAAGFLPTHVE
                     QFFGKQKGAKAPPDLGDRLRSLLERAEGFELSSALVIDAPTDDKAQTVRAVLENDGYV
                     VSQLGRRLYVSIGTQRSASIDVERIHARLVATGLGSVTNRTRT"
BASE COUNT         1078 a         1974 c         2119 g         1086 t
ORIGIN      
        1 tcgatgagtt gcgccgggaa gcagccgagg gcgccgacct gatgatcaca atcggcgggg
       61 cgtcgctcgg cgatcgtgat gtgctgacgg agctggccag cgatatggtt gcgctcgatt
      121 gctggaatat cgcgatgaag cccggaaagc cgctggttta cggtcagatc gatggcgcgg
      181 tcgtgatcgg gctacccggg aatcccgccg ccgcattcat ctcggccgtg cagttcgtac
      241 ggccggtaat cgcgaccatg ctcggccgcc ttgacatcga tccgcccacg gtcgtcgccc
      301 gtgctacgga atcggtcaca aactccggtg gtcgccgcaa ttacctgcgg gtgacgctcg
      361 aggtcgaaga gtccgggtat gtcgcaaggc tggccggacc acaaaacgta gctagtctcg
      421 caacgctgag ccgcgctgag ggtctgctga tcgtcccgga agggatcgag cgggtcgagg
      481 cgggcgagcg atttgacgtg cagatcatcc ggacgctgta gcacgaagtg tgccggacgc
      541 acatcttcgg ttcatcttca atcgctatcc tcgatggttg ggccatctcc ggtcctgaaa
      601 tccaccgtca aatcgaggag gatgacgatg acgaatcgcc ccgttttgcc ctcagtttca
      661 cgacggacgc tcctgcaggc gtcggccgta ggtgtcgcgg cagtgcccgt cctggcctat
      721 gcgcagaact cgaccccaat tgccagtccc gtatcgagcc cggtcgcgcc gccgccggcc
      781 gggagtcccg ccgcgagtcc agcagccagc ccgacgccgg aagtgacggt catcgaatgg
      841 ccgacctacg ggcaggatct gaccggcgac aaggcgatcc ccgacggcgt gatcaccagc
      901 cagaacgtag ctggcctgac tgagctctgg accgtagatg tcggtggtcc gatcagcgcg
      961 acgccagtta tctcgggcgg catcgcctac atcggctcgt acgacggcac gctctacgcg
     1021 atcaacatcg cgtccggcaa tgtgatctgg acctacgaca ccggtgcggc cgttgaggag
     1081 ccgaacctga agatcaacct cggcgtgacc ggctcggccc acgtcgaaaa cggcgtcgtc
     1141 tatgtcggcg acgcggcggc gatggtgcac gcaatcgaca ctgcgaccgg gcaggcgatc
     1201 tggacccgga aggtcgatga ccaggagcac gccagcatct ggtcgtcgcc ggtggtctgg
     1261 aacgggacgg tctatgtcgg tgtggcgtcg gtcgcgaagc agattgggtt ccggggcagc
     1321 gtggtcgcgc tcaatgccga atcgggcgag caggtttgga agacgttcat ggtgcccgag
     1381 gatgccgatg gcgccggcgt gttcgcggtc ccggcgatcg acgagcaacg cggcatgctc
     1441 ttcgtcggca cgcagaacgc ctactccgag agcgatgcgc cgtacggcaa cccgatcagc
     1501 atagtcgcgc tgaacgcgag cagcggggac atcgcctgga cgtacaacgc gcctccgaat
     1561 gacggcaaca aagcgccgac ggacgatgtc ggcttcagcg cgtcgccgaa cctcttcacc
     1621 gccacgatcg acggtgtcga ccgtgatctc gtcggcgagg ggcaaaagag tggcgacttc
     1681 atcgtgctcg atcgcgagac cggcgagaag gtctggaccg cgacggtctc gccggccggc
     1741 ttcctcggcg gcatggaagg gacgagcgcg gtcttcaacg ggatcatcgc cgttcccgcg
     1801 accgactggc aggatccaag cggacagggc accggcatgg tgaccgggct cgacgcgaca
     1861 accggagagc agcgctggac tgcgcagcag gatgctccgg ccccggcccc ggtggcgatc
     1921 agcaacgatg tcgtcttcca cggcggcttc gacggcgtgc tgcatgccta ctcgctgact
     1981 gacggtacgg agctgttcac ggccgatctc ggcgcctcgg tcagcggtgg cacggctgtc
     2041 gccgacggcg tcgtgatcgt cggcgcggca actccgcagt tcgccgactt catcaagacg
     2101 ggcaaccaga tccatgcctt cgggttcatc gccgaagcca ctccggccgc ctctccaacg
     2161 acggaagagc aggtggcgtc gccgacggcc gttccgccga ccgaggcgcc ggtgacctct
     2221 ccaacggccg aggctccgac ggagactccc tcgccaacgg aggaagcctc gccggtcatc
     2281 gcgtagggat cgaagcggga cgcgggatga ctgcgtcccg ccggtccgtg atgccgtcga
     2341 ggggttcggc taggaccggg ccttttcgat cgcgtcgcgg atctgcgctt cgtccatggg
     2401 gccaaccgtg accttgtcga tgacgccgtc tggccgaatc acgacggtca ctggatacga
     2461 gccgccggtg ccgagcgcag tttcgatagg accgtaaagc gggttggcgc cgccgtcgtc
     2521 ccgcgcgatc tgataggtga cgccggtctc ggcgaggagc tcggttgcgt tctcgacccg
     2581 gtcgttcttg atattgacgc cgatgatccg cacgtcaccg ccggcgctct ggtggactgc
     2641 ctcgaaggcg ggcatctcgg tccggcacgg ctcgcaccac gacgcccaga aattcagcac
     2701 cacgacatcg cctcgcatcg aggccagatc ggtcggcgtg ccgccgtcga agccggtgaa
     2761 ggtcagattg ggcgcgggct gaccgatgat ttcccgcgtg gtcgtcttct tgtcgcgctg
     2821 gttgatggcg atgaacacga tcgcggcgac catgatcgcg gccagcacga tcggcgagta
     2881 ccttgccagt ttcccgtagc caacccggcc gtactcatcg aggtcgggtt ccagctcgga
     2941 gcggtcgatc atgtcagccc ttcgacagcg gccttgaccc ggtcggccgt ctgctggtcg
     3001 gcatcgacga gcactaccgc gacattgctc ccggcgtaca ggtcgatcga ggtgtactgg
     3061 acgtcgtcgc cgttgatgtc cacgaggtcg acatcctccg gctgcacgtc gagcgttgcc
     3121 tgctcctgcg agccaacgtc gggatagatg aagacgtagg cacgtgccga gccgaccttg
     3181 atcatctggc cgggccgctc gagcatggtc gagcgcacat cggtgccttg ctcgccgaac
     3241 tcggcgctca ggttctgggt gcggaaggca tcgagcacgt cgatcgcctg gttgtgaccc
     3301 ggcaccggag tcggcggctt cggctcgtcg ggctggttgc gatcgcgcac atagagcacg
     3361 acgacggcga ccaggagtgc tgcggatatc gccagcagca cggtcgagat gatcgattgt
     3421 gcgcggcggc ccatgcggtg tggtcgactc ccgacgttgc cctgcggtgc ggcaggataa
     3481 taggagagtg tacgtcgtcc ctaaacgccc ggacgcgcac gatcgcccga cgactcggca
     3541 ggaggccgga tggaccgacg cgaattcctg atgggatttc cgctccttgt cgccggcacg
     3601 ccggaggcag agagcggctc gtgggtccgg cgcgcggata tgccggtggc ccgctcggag
     3661 acgccggcag cggtgctgga tggcaagatc tacgtcgcgg ggggcttcgg ggccggagcc
     3721 cgggcagatc gatacgaccc cgccaccgat cagtggcagc agttggccga tctgccggtc
     3781 gagacgaatc accccgggat cgcggtgctc gacggtcacg tggttgtcgc cgggggttac
     3841 gcgatggacg gtgccagcgc gcacaatggg atgtgggcgt accaccccgg cgccgattcc
     3901 tgggaggtga tcggcgagtt gcccgaggcg atgggagcat tcggcctagc cacggttgat
     3961 ggcgatctct acctggtcgg cggcgcgttc ggatcgctca acggcgagcc gtcggcggcg
     4021 gtctggcgct ggcggccgca ggacgggacg tgggagacgc gcgctccgct tgcccaggcg
     4081 cgggaacacc ttgcggtcgt ggctgtggcc gggaagatct acgcggtcgg cgggcgcgcc
     4141 cacgggaggg acaacgatca gctcggcggt gccgtcgagc ggtacgatcc ggccgcagac
     4201 aaatgggact cgctggcgtc attgccgcat ccccgcagtg ggctcaatgg ggctaaggtg
     4261 tgtgaaaccg tggtcgtggt tggcggggag accagcaagc gcgtattcga cgacgtccag
     4321 atactcagtc tcgacggcgc aagttgggca tcgctgcccg atctgcccct cgcggttcat
     4381 ggcgtcgcgg tcgcttcggt caataatcgt ctgtttgcga ttggcggctc gacgctggcc
     4441 ggtcggatcc agaacgtcac tgccgtgtat caacttgacc tcggaaccac acgcggcgct
     4501 tgtgcgtcct aatgttcgaa gagtggcgga tttcgcaatc aattcctctt tgataaagag
     4561 agggactggc cgaattgcgt gaattctgct tatattgctc aaagtatggt cgacgtcaga
     4621 aacgccgccg cagcagggtg agagagtagg gaatggtcgt atcgttaacg gaaaataccc
     4681 gttcggcagc ggaactcgca atccgcgtaa ccccccaact cagccgttgg atacagctgc
     4741 aaatgacgaa tgatgaggaa catgacctca gcatgcgcca gttgagtgca ctgcaattta
     4801 ttgacggcac cgagacaacg cttggagatg tcgcccgcaa cctgaatgtg actcccgcgg
     4861 tcgtcaccgg cttgatcgac cggctcgagc ggcgcggcta tgtccgccgc gtggccagcg
     4921 tgtttgaccg ccgccgggtc tacatcgagc tgaccacggc cggtgaagac gtccgcgacc
     4981 acgccgaaga ccagcttgcc aagcggctcg atgatctgct ctcgaagctc tccgacgacg
     5041 aggtcaccgc gctcgaggat ggactgcggg tgatggagaa ggtcatggcg tcggtcgagc
     5101 cgcgccggaa caagcctggg atcaagtccg agttcggttc gtaaccgatc ccaatcccgt
     5161 cgcaaccagc cgcgcatgga tccgctcgac atcgatcgag gcggatcgct gcgtcccgat
     5221 actgacgtag agccggcgtc cgagttggct gaccacgtaa ccgtcgttct ccagcaccgc
     5281 gcggacggtc tgcgccttgt cgtccgtcgg cgcgtcgatc acgagcgcgc tcgacaactc
     5341 gaatccctcc gcccgctcga gcaatgaccg gaggcggtcg cccagatcgg gcggagcctt
     5401 tgcgcccttc tgcttgccga agaactgctc gacgtgcgtc ggcaggaagc cggcagcctg
     5461 ggccgtgcgg aggctgtccg gggtaatcct gaagacactc tcgcgaccga gcgagagggc
     5521 atccgcgaag gcgagcacgg accagacgtg gatcggctct ggatcggtca ggtggatcgt
     5581 cagatcatca gcgacggcga tggagggcgc tgctcgggac gcggctcgat cttcgacctc
     5641 cgcccgcgtc acccggcgca gctcatcggt gaccagcatc agccgctcgt tgcgacccgg
     5701 ctcatgcagc cgcacgaaac ccagccagac cagaatcgac tggatcacgg cggcggccag
     5761 gtaggcaacg ctttcctggt gcgggctttg gccgggctgg gcggtggcgt ggctgaccgc
     5821 taccgtggcg tctggcccga cgatggcggg gtcgtactcg ctgatccatt gcgctacgtc
     5881 ggcgacgcgg taccacttgt ctttttcgag cgccgcgagg tgactgagca gcttgacccg
     5941 gaagccgcgc caatccacat tccacggctc gacatcctgc cggccctgcc cctcgatcca
     6001 gaccggcgag ctcatccaga tgctccggat ccgcgcggtc tgatccgccc agctcttggt
     6061 ccgccaatcc cgcatcgccg atgtccgctc gaactgggtc gagccgtcct ccggctcctg
     6121 gatcaggccg aggttgaccg cgaagtccac cagcaactcg aggtagccga tcggcgggcg
     6181 atccgggccg cggttccaga gcatccggtt gatctcggtc aggtaggagc ggggcgcggc
     6241 cagcggatcg agattgg
//
LOCUS       DATAOZ010000679         4289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U7058
            k141_4470629, whole genome shotgun sequence.
ACCESSION   DATAOZ010000679 DATAOZ010000000
VERSION     DATAOZ010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955671
            Sequence Read Archive: SRR12927892
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 04:32:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,419
            CDSs (total)                      :: 3,385
            Genes (coding)                    :: 3,373
            CDSs (with protein)               :: 3,373
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 1 of 12
            Pseudo Genes (incomplete)         :: 9 of 12
            Pseudo Genes (internal stop)      :: 3 of 12
            Pseudo Genes (multiple problems)  :: 1 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4470629"
                     /isolate="SMAG_U7058"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.0633 N 88.2058 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="VHR64_17080"
     CDS             <1..1328
                     /locus_tag="VHR64_17080"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX3304604.1"
                     /translation="SAEIEPALAESWTNSEDGLSYVFTLRSGVTFHDGTPLDAKAVVT
                     NFNRQLDENDPLHDPGMVYAGVILQDVTKVEATGDLEVTLTLGRPIKLVPANLAVFAA
                     GIVSPTALETYKDDFSNHAAGTGPFKLDHWTKDVELVFVANDQYWGGRPKLDRVIWRT
                     IAEDTVKLQELTTGSIDVANQLDFKDIDTVKNDSNLQLVSGPFWNVQFLAVNQAVAPF
                     DSAQVRQALQYAINKQNIADVVFYGNYTLGAGPIAPGLLGYDEALQSTYTYDPEKAKS
                     LISESGVSDISFDLYNRTNSVWPLIGQLIQADLEAVGIKANIVALEDAEFFSQLGTGK
                     TAAFLNDWTWDNGDPDNVTYSLFSAPRAETRLGYKNDRVNELNTLAQEEADQNKRAEY
                     YAEMQQLILQDAINVFLGYPSRAIGAVAKVQGLVLSPIGNIVLRDVDVS"
     gene            1548..2783
                     /locus_tag="VHR64_17085"
     CDS             1548..2783
                     /locus_tag="VHR64_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX3304605.1"
                     /translation="MVDWYVGETRWDRVRDAIGRHGFDALLAVTPENAEYLAGQSNFI
                     ATHWRLPGIHAATIGSGHRAVVTGDFGVDPTAARAYTHIPYRSWTESIDIRGLLEGRL
                     SDRVVAARPGVPIERPAQFDLDAVFDAVAEAVRAVASIPRRVGVDLLEVDAASVGRLR
                     TRLPGVQLVDATAVFDDLRAIKDPDEIAHLRLAGELTEVGIRGALARLSPGMSETALN
                     AAYQVAVQERVIADERFAAFRQAEGMANIGIGADSPHAVVPGQTLKFDMQVDIAGYHS
                     DVGRTFAIKPTADQRAMYGALRDALAVLEDAIRPGVTFAELYATGSGAMHRAGFTNYS
                     RGHLGHSLGLTQRFEEPPFIAPGEERPVVPNMVLAIEMPYYVYGVGAFQLERMGVVTE
                     TGFELVDQLPFELELQLPG"
     gene            2802..3806
                     /locus_tag="VHR64_17090"
     CDS             2802..3806
                     /locus_tag="VHR64_17090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX3304606.1"
                     /translation="MARYILRRVLLAIPILVGISLIVFLMLHSAGGDPAEVKLGARGD
                     QASIAALRHEMGLDRPLAVQYLDFLSGAIHGDLGRSYRSNAPVADEVFSRFPATIELA
                     VAALLIGGTIGFMAGVLAATRRGSIFDFASTFGALIGVSIPTFWLGLILIIIFGVWLQ
                     WLPISGRVNPRLGADPNAHFLILSSLVHGDWVIFKDAVRHIILPALTLAGWPAAIIAR
                     MTRASLIEALDQDYIRTARAKGLTEQRVVRSHAFRNALIPIVTVVGLELGGLLGGAVV
                     TETVFAWPGVGKLTADAIAARDYQVTQGVVLLLGAVFVAINLLVDLFYAFIDPRIRYG
                     "
     gene            4062..>4289
                     /locus_tag="VHR64_17095"
     CDS             4062..>4289
                     /locus_tag="VHR64_17095"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX3304607.1"
                     /translation="MTARTGAQAIGAQGAANAMPEVRRGPSFWDRFKRNRLAVVGLAI
                     LVVLGVAAVIGPPLVDPPRKQNVVERFERPSI"
BASE COUNT          722 a         1280 c         1505 g          782 t
ORIGIN      
        1 gatcggccga gatcgagccg gctctggccg agagctggac gaactccgag gacggcctct
       61 cctacgtctt cacgctccgc tcgggcgtca ccttccacga cggcacgccc ctcgatgcca
      121 aggcggtcgt gaccaatttc aatcggcagc tcgatgagaa cgatccgctc cacgacccgg
      181 gcatggtcta cgccggcgtt atcctgcagg acgtgaccaa agtcgaggcg accggcgacc
      241 tcgaggtgac cctgacgctg ggacggccga tcaagctcgt cccggccaac ctggccgtct
      301 tcgcggccgg catcgtcagc ccgaccgcgc tcgagaccta caaggacgac ttcagcaacc
      361 acgccgcggg gaccggtccg ttcaagctcg accactggac caaggatgtc gagctcgtct
      421 tcgtggccaa cgaccagtac tggggcgggc ggccgaagct ggatcgcgta atctggcgga
      481 cgattgccga ggacacggtc aagctccagg agctgaccac cggcagcatc gacgtcgcca
      541 accagctcga tttcaaggac atcgatactg tcaagaacga ctccaatctg cagcttgtca
      601 gcggtccgtt ctggaatgtc cagttcctgg ccgtgaacca ggcggtcgcg ccgttcgaca
      661 gcgcgcaggt gcggcaggcg ctgcagtacg cgatcaacaa gcagaacatc gccgacgtcg
      721 tcttctacgg gaactacacg ctcggggcgg gtccgatcgc gccgggcctg cttgggtatg
      781 acgaggcgct ccaatcgacc tacacctacg accccgagaa ggcgaagtcg ctgatctcgg
      841 agtcgggagt cagcgatatc tcgttcgacc tctacaaccg gacgaattcg gtctggccgc
      901 tgatcgggca gctgatccag gcggacctgg aggcggttgg gatcaaggcg aacatcgttg
      961 ccctcgagga tgccgagttc ttctctcagc ttggcacggg aaagacggcg gccttcctga
     1021 acgactggac gtgggacaac ggcgatccgg acaacgtgac gtactcgctc ttctcggcgc
     1081 cgcgggcgga aacccggctg ggctacaaga acgatcgggt caacgagctg aacacgctgg
     1141 cgcaggaaga ggctgatcag aacaagcggg ccgagtacta cgccgagatg cagcagctga
     1201 tcctgcagga cgcgatcaac gtcttcctcg gctatccgag tcgggcgatc ggcgcggtgg
     1261 ccaaggtgca ggggctggtg ttgtcaccga tcggcaacat cgtgcttcgg gatgtagatg
     1321 tgagttaggc gcggagtttt agggcgagca ggcgagcggt tctgaacgta gtggccgggc
     1381 tccgtacccg cctgggctcc ccaccgaccg ctctgcatgc accggatctc tcgtaacggg
     1441 tcggacacca cgcagcaact ggttacgcca ctcaaggcgg gtacggagcc ccgccactac
     1501 gaatctcagc tcgggtcttc aggcttgggc cgagcgacgg aggctcaatg gttgactggt
     1561 acgtcgggga gacgcggtgg gatcgggttc gggatgcaat cggccggcat gggttcgatg
     1621 cgttgctggc ggtgacgccg gaaaacgctg agtacctggc tggtcaaagc aacttcatcg
     1681 cgactcactg gcgcctcccc ggcatccatg ccgccacgat cggctccggc catcgggcgg
     1741 ttgtaaccgg cgacttcggt gtggatccca cggcagcgag ggcatatacg cacatcccgt
     1801 accggtcgtg gactgaatca atcgatatcc ggggacttct cgagggtcgt ttgtctgatc
     1861 gcgtcgtagc ggcgcggcca ggcgtaccga ttgagcggcc ggcgcaattt gacctcgacg
     1921 ccgtgtttga tgcagtcgct gaggcggtac gggccgtcgc gtcgattccg agacgggtcg
     1981 gggtcgactt gctcgaggtt gatgcggcgt ctgtgggacg gttgaggacc cgcttgcccg
     2041 gagtccaact ggttgatgcg acggcagtgt tcgatgacct gcgcgcgatc aaggatccgg
     2101 acgaaattgc gcacctgcgg ctggccggcg agttgaccga ggtcggaatt cgcggcgcgc
     2161 tggcccgcct gtcgcctgga atgagtgaaa ccgcgctgaa cgccgcctac caggtggcgg
     2221 tgcaggaacg ggtgattgcc gatgagcggt tcgccgcgtt ccggcaggcc gaggggatgg
     2281 cgaatatcgg aatcggggcc gactcaccac acgccgtcgt gcctgggcag acgctcaagt
     2341 tcgacatgca ggtcgatatc gccggatatc acagcgatgt tggccggaca tttgcgatca
     2401 agccgacggc ggaccaacgc gcgatgtacg gagcgcttcg ggatgcgctg gcggtgctgg
     2461 aagacgcaat ccggccgggc gtgaccttcg ctgaactcta cgcgaccgga tccggggcga
     2521 tgcaccgggc tggcttcacc aactacagcc gtgggcacct tgggcacagc cttggcctga
     2581 cgcagcggtt cgaggagccg cctttcatcg cgccgggcga ggagcggcct gttgtcccga
     2641 atatggtgct ggcgatcgag atgccgtatt acgtgtacgg cgtcggcgcg ttccagctcg
     2701 agcggatggg cgtcgtgacc gagaccggct tcgagctggt cgatcagctc cccttcgagc
     2761 tcgagctgca gttgcccggc tgacgcggaa ggacccagcg cgtggctcgg tatatcctgc
     2821 ggcgggtact gctcgctatc ccgatcctgg tcggcatctc gctgatcgtt tttctgatgc
     2881 tgcattcggc cggtggcgat ccggccgagg tcaagctcgg cgcgcgcggc gaccaggcgt
     2941 cgattgcggc gctgcggcat gaaatggggc tggaccggcc gctggcggtg cagtacctcg
     3001 acttcctgtc cggcgcgatc catggcgatc tcgggcggtc gtaccggtcg aatgcgccgg
     3061 tggccgacga ggtgttttcg cgcttcccgg cgacgatcga gctggcggtg gccgcgctgc
     3121 tgatcggcgg cacgatcggc ttcatggccg gagtcctggc agcgacgcgg cggggctcaa
     3181 tcttcgactt tgcgagcacc ttcggggcgc tgatcggcgt ctcgatcccg acgttttggc
     3241 tcggcctgat cctgatcatc atcttcgggg tctggctgca gtggctgccg atctcggggc
     3301 gggtcaatcc gcgtctcggg gctgacccga acgcgcactt cctgatcctg tcgtcactgg
     3361 tgcatgggga ctgggtgatc ttcaaggacg cggtcaggca catcatcctg ccggcgctga
     3421 cgttggccgg gtggccggcg gcgatcatcg cgcggatgac ccgggcgtcg ctgatcgagg
     3481 cgctggatca ggactacatc cgcacggcgc gggcgaaggg tctgaccgaa cagcgggtgg
     3541 tgcgcagcca tgccttccgg aacgcgctga tcccgatcgt gaccgtggtc ggtctggagc
     3601 tcggcgggct gctcggtggg gcggtcgtga ccgagaccgt gttcgcctgg ccgggggtcg
     3661 ggaaattgac ggccgacgcg atcgcggcgc gggattacca ggtgacgcag ggggtggtgt
     3721 tgctgctggg ggcagtgttc gtagcgatca acttgctcgt ggacctgttc tacgcgttca
     3781 tcgacccgcg cattcgatac ggatgatggg cgtggaaatg cctatatacg ggatttcgga
     3841 aagggctaac cgggcgagcc gcccggcact acgtttaacg agtccgggcg gcgtttccgg
     3901 atgtagcgtg gggcggctcg ccccgcgatt gccggaactg ccatgatcgt tgtcgcgaaa
     3961 acggttgatc tcatggcatc tagtcgatcc gcgtcgcccg gggctaaagg cccgggctac
     4021 accacgataa gcccactaaa ggggctgaat ggggcgttga agtgacggcg cggacagggg
     4081 cgcaagcgat cggggcccag ggcgcggcta atgccatgcc ggaggtgcgg cggggaccgt
     4141 cgttctggga tcggttcaag cggaaccgat tggcggtggt tgggttggcg atcctggtgg
     4201 tgcttggggt ggcggcggtg atcggtccgc cgctggtcga tccgccgcgc aagcagaacg
     4261 tggtggagcg gttcgaacga ccgagcatc
//
